LOCUS       DASUMX010000001         7268 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1007282, whole genome shotgun sequence.
ACCESSION   DASUMX010000001 DASUMX010000000
VERSION     DASUMX010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 7268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7268
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1007282"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="VFZ86_00005"
     CDS             <1..295
                     /locus_tag="VFZ86_00005"
                     /inference="COORDINATES: protein motif:HMM:NF024639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEX5640704.1"
                     /translation="DTAEHKPSPVPLHLCLERFGASAQGALYVGDSPVDIEAGRAAGM
                     ATAAVAWGVFGPEALLAAGPDYWLDEPAELLGLCLRGEGRRVSAGQGPGTTSG"
     gene            334..2493
                     /gene="ligA"
                     /locus_tag="VFZ86_00010"
     CDS             334..2493
                     /gene="ligA"
                     /locus_tag="VFZ86_00010"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEX5640705.1"
                     /translation="MNQAQAAARAAELRATINRHAHLYYVLDRPEIDDAAYDVLYREL
                     QTLEEEHPELRTPDSPTRRVGAAPLEGFTQVRHLEPMLSLANARDEEELLAWDQRNRR
                     LLEARELGDAPLRYVVEPKIDGLAISLTYREGVFSVGATRGNGEVGEDVTANLRTIGS
                     VPLRLLLDDPPPVVEVRGEVYLPLAAFARLNEQRTADGLPAFVNPRNSAAGSIRQLDP
                     RLAAARPLDVWCYAVGYSEGLDLADHHSAIEWLRAAGFRVNPLIVTVDGIAEAAAACR
                     AWEERRGDLDYDIDGAVVKVDSYTLQRALGAVAHDPRWAIAFKFAPTTVTTRLHRIEV
                     NVGRTGVLTPFAVLEPVFVGGVTVERATLHNEDDIRRKDIRPGDDVIVQRAGDVIPQV
                     VGPVTAGEAEDEEGRREGREERHIALPEWTMPRTCPACGSETVRETGEVAVRCPNRSC
                     PAQLVESIKHFVSRGAMDIEGIGEETVELLHGKGLIENVADLYGLEREHFVRLEAGKV
                     IALPGFGGKVRKGEDGAPELVEARRADKVLTAIEQSKQRPFARVLYALGIRHVGAVTA
                     QALVERFPSMDALLAAGAADLAEIPGIGGVVAEAVEQFLGDEHNRETIEKLRAAGVRL
                     VEDSPARAEGPLTGNTFVLTGKLPTLTRGQAQAIIEAAGGKVSSSVSRATDYVVAGEE
                     AGSKLDKAAALGVPVIDEAGLLEMTGDDDDGDASLFD"
     gene            3207..4568
                     /gene="mtbB"
                     /locus_tag="VFZ86_00015"
                     /pseudo
     CDS             3207..4568
                     /gene="mtbB"
                     /locus_tag="VFZ86_00015"
                     /inference="COORDINATES: protein motif:HMM:NF021053.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="[dimethylamine--corrinoid protein]
                     Co-methyltransferase"
     gene            complement(4584..5528)
                     /locus_tag="VFZ86_00020"
     CDS             complement(4584..5528)
                     /locus_tag="VFZ86_00020"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEX5640706.1"
                     /translation="MSSRSSGGAALARSARTAALLAVLALAVAALVLGLAACGDEPSS
                     AEPAVTGTAPPEVKAQTGILLVRDSGEVLWSKHPDREMAPASCTKIMTALLTLEVVDD
                     LDAMATVPDIPLPQNVGVDLVPGDRISIREALQALMVKSANDAALTLASYVGGSEKGF
                     VKLMNERAAQLGLTHTHFLNCRGTPEDGHYSSARDLATLGRFAMQDATFRELVRTKTA
                     VIRYPPDAAVPVESHNRLLDYPWGDGIKTGATDISGKVLVGSGKPGAVALIVVTMHEP
                     TRDQEEKDAVALFTWGTAEYLRRSAASTSPASAASPSP"
     gene            complement(5525..6703)
                     /locus_tag="VFZ86_00025"
     CDS             complement(5525..6703)
                     /locus_tag="VFZ86_00025"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEX5640707.1"
                     /translation="MASRIRTQHRARLYIAAACLLLLAAGAFVAAVATRAPSQAAADA
                     QSVSAEGAATTAQAAPGVRARSGILIDRRTGRVLWSKSADLRLAPASCTKIMTALLVL
                     ERYSNLERMVSAPGSVRDFQQVAIGLRPGDRISVKQALRALMTKSANDACVTLATAVG
                     GSEAGFVKLMNRRARQLGLTHTRFVNSRGTPKPGHYSSARDLARLGRYAMRDARFRDL
                     VGTKTRVITWPPSHRVTVTSHNRLLDYPWGDGIKTGSTKQSLRVLVGSGKPGLVPLIV
                     VTMREPTRDQEERDAVALFTWGSALYESRQIVAAGEVVKQLQVDGDAPVDAVAGADLT
                     ATIRKAAAPTLVFDLPQEPLPARPADGDVLGTVTYRSDGLVLGRVDLLAATPAGPAAQ
                     "
     gene            6762..6837
                     /locus_tag="VFZ86_00030"
     tRNA            6762..6837
                     /locus_tag="VFZ86_00030"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6795..6797,aa:Thr,seq:tgt)
BASE COUNT         1008 a         2537 c         2694 g         1029 t
ORIGIN      
        1 cgacaccgcg gagcacaagc cctcgccggt gccgctccac ctctgcctgg agcgcttcgg
       61 ggcgtccgcc caaggcgcgc tctacgtggg ggacagcccc gtggacatcg aggccgggcg
      121 cgccgccggc atggccacgg cggccgtggc ctggggggtg ttcgggccgg aggcgctgct
      181 cgccgccggc ccggactact ggctcgacga gccggcggag ctgctcggcc tctgcctgcg
      241 cggcgagggg cgccgcgtga gcgccgggca agggccgggt accaccagcg ggtaagcggt
      301 cgcggcctcc tgcggccgcg gaagggggaa gcggtgaacc aggctcaggc ggcggcccgg
      361 gcggccgaac tgcgcgcgac gatcaaccgg cacgcgcacc tctactacgt cctcgacagg
      421 cccgagatcg acgacgccgc ctacgacgtc ctctaccgcg agctgcagac gctcgaggag
      481 gagcacccgg agctgcgcac gccggactcg ccgacccggc gcgtgggcgc cgcgccgctc
      541 gaggggttca cgcaggtccg ccacctcgag ccgatgctct ccctggccaa cgcgcgcgac
      601 gaggaggagc tgctggcgtg ggaccagcgc aaccggcgac tgctcgaggc gcgtgagctc
      661 ggcgacgcgc cgctgcgcta cgtcgtcgag cccaagatcg acggcctcgc catctcgctg
      721 acgtaccgcg agggggtgtt cagcgtcggc gccacgcgcg ggaacggtga ggtcggcgag
      781 gacgtcaccg ccaacctgcg caccatcggc tcggtgccgc tgcggctgct cctcgacgat
      841 cctccgccgg tggtggaggt gcgcggcgag gtctacctgc cgctcgccgc gttcgcccgc
      901 ctcaacgagc agcgcacggc cgacgggctc ccggcgttcg tcaacccacg caactcggcc
      961 gcggggtcga tccgccagct cgaccccagg ctcgcggcgg cgcggccgct cgacgtgtgg
     1021 tgctacgcgg tcggctactc cgaggggctg gacctggccg accaccacag cgccatagag
     1081 tggctgcggg cggcgggctt cagggtcaat ccgctcatcg tgaccgtcga cggcatcgcc
     1141 gaggccgcgg ccgcatgccg cgcctgggag gagcgccgcg gcgacctcga ctacgacatc
     1201 gacggcgcgg tggtcaaggt cgattcgtac accctccagc gcgccctcgg cgccgtcgcc
     1261 cacgacccac gctgggcgat cgccttcaag ttcgcgccga cgaccgtcac cacgcggctg
     1321 caccggatcg aggtcaacgt cgggaggacg ggcgtgctca ccccgttcgc cgtgctcgag
     1381 ccggtgttcg tcggcggcgt caccgtggag cgcgccaccc tgcacaacga ggacgacatc
     1441 cgccgcaagg acatccgtcc cggcgacgac gtgatcgtcc agcgcgccgg cgacgtgatc
     1501 ccccaggtcg tgggaccggt gacggcgggg gaggccgagg acgaggaggg gcggcgtgag
     1561 ggccgcgagg agcggcacat cgccctcccg gagtggacca tgccgcgcac ctgcccggcc
     1621 tgcggctcgg agaccgtgcg cgagacgggc gaggtggccg tgcgctgccc caaccgctcg
     1681 tgccccgccc agctcgtgga gagcatcaag cacttcgtga gcaggggtgc gatggacatc
     1741 gagggcatcg gcgaggagac ggtggagctc ctccacggca aggggctgat cgagaacgtc
     1801 gccgatctgt acggcctcga gcgcgagcac ttcgtgcggc tcgaggccgg caaggtcatc
     1861 gccctgccgg ggttcggcgg caaggtgcgc aagggcgagg acggcgcgcc cgagctcgtg
     1921 gaggccaggc gcgccgacaa ggtgctcacc gccatcgagc agtccaagca gcggccgttc
     1981 gcgcgcgtgc tctacgcgct gggcatccgt cacgtgggtg cggtgaccgc ccaggcgctg
     2041 gtggagcgct ttcccagcat ggacgcgctc ctcgccgcag gagccgccga cctcgccgag
     2101 atccccggca tcggcggggt ggtggccgag gcggtggagc agttcctcgg cgatgagcac
     2161 aaccgagaga ccatcgagaa gctgcgcgcc gccggtgtcc gccttgtgga ggacagcccg
     2221 gcacgtgcgg agggcccgct cacggggaac acgttcgtgc tcaccggaaa gttgccgacc
     2281 ctcacgcgcg gtcaggccca ggcgatcatc gaggcggcgg ggggcaaggt gagcagttcg
     2341 gtgagccggg cgaccgacta cgtcgtcgcc ggcgaggagg ccgggtccaa gctcgacaag
     2401 gccgccgcgc tcggcgtccc cgtgatcgac gaggccggcc tgctggagat gacgggcgac
     2461 gacgacgacg gggacgcatc cttgtttgat tgactttgtc cagtctgcgg ccggccgtgg
     2521 tcgtgaacct acctgaaacg gacaccgtag gaaggctgat aggactgcgc gcttcatatc
     2581 ggttaatatt tggttaacga ttgctcaata catcccccct agtgtccccc ttgcgacgct
     2641 tcccgtctcc gagaagcccg ctgccagcgc cagttgctgg tacctcctat cttcttggtt
     2701 cgccccggcc ccgggcgaac cacggcctga gggccggcgg ggcggccgct tccaaagcga
     2761 cccggctcgc cggccttcgg ccatttcagg aaacggatcc actacccacc cgcggtgcgc
     2821 gctgccgtgt aactgtccgg caaccttctc ttcaagcgtg ttcgcaccgc ttcacgccct
     2881 ctcaacattc cccgggcaga ctgtgggtgg ctcctttcgc tggcaagcga aaacccccca
     2941 cgagggtttg ccccccaagc ccttgtacga ggccccgttc cccgcggggc ctcgcccctt
     3001 taagcgcgcc gcaccctttc gatccgctgc gatacgcgcc cggtgccggc gtttggcctg
     3061 cagcgccgtc aactttgccg aagtatcgac cgctgacagc cggcagcccg gcctgtagcc
     3121 tcggtgtgcg gcagctgcgc tggacggagg cgcgcggtcg cgggcaccac gagtcacacg
     3181 gaggcacgat gaggatcccg acgcgcatgg gcgatgggtc gctggtgcgg atgacgcggg
     3241 cggagatcgc ggcggaggtg gccgccggag tcgaggccgg cgtgaagcgt gcgaaggtgg
     3301 cgccgctcgg cgacgacgcg gtcgcgcacc tggtcgacgt cttcgcgagc acggcccgct
     3361 tcagcgccgt ggacatcggc gacgaggtcg tcctgagctc ggacggggtc ggcagccagg
     3421 acgtggggtc gcgcgtcaac gacctgcaga ccttcgagca gacggtgtgc accgacaccg
     3481 tggagctcta ccaccacgac tactccttca aggcgatcaa gccggtgctg ccgttcgaac
     3541 aggcgaccat gcgccgggcc cagctcctcc tcaccgtccc ggtacagtac ggcgccatgc
     3601 ccgacctcgg ccggtacacg gtccccgacg gccccgtgcc caactggtcg cagctgttgc
     3661 cgctgggccg catcgacgag gcgcgggcgg cccaggagga ggccgtggag caggctgtgg
     3721 acgacatcgt ctccgtcgcc gagggcatgt acgaggcggg cgccgacggc atcaacctgg
     3781 acacggcggg ggcggccggc gacgccgact tcctggccgc cctcagggcg atcgagcgca
     3841 tccgctcgcg gcatcccgtg tggggcatcc agatcggcat ggcgagcgag ttcgtgctcg
     3901 gcatgcacgg cgagctcgag tacgaagggg tccggctcgc cgggctctgg cccgccggcc
     3961 agatgcgcct ggcggccatg gcgggcgcct cggtcttcgg gcccgccgtc aacgtcaaca
     4021 ccggcaagtc ggtcgcctgg aacgtggcgc gcacgtgcac catcgtcaag ccgtgcaccg
     4081 acgccgcctc tctccccatc cacctcaacg gagggatggg ggtgggcggc gtccccatgc
     4141 acgcgctgcc gcccgtggac gccgtctcgc gcgcctcgcg cgcgtgcgtc gacgtcctgc
     4201 ggctggacgg cttgtagatc ggcgccggcg acgaccacag ccaggggatc ggccacggtg
     4261 tcgcgtcggg catgggcgga ttgcggaccg ccggagacct ggtggcccgc atgcagatga
     4321 cccggcggat gcggctcccc gaggccaagg cctacgtggc ggagcggctc ggcgtgacgc
     4381 cggccgatct cagtgacccc gtggcgatgg ccgaggtgcg tcgcgcccgc ggtctcggca
     4441 cgctcccgac cgaggacacg gcgacggtgg acgacgcgct ggcgatcgag gccaagctga
     4501 acatcgcccg cgtgctggac gtgccggtgc gctcggtgga gcggctgcgg gcgctcgggc
     4561 gcggctgagg cgccggacgg gcgtcagggc gagggcgacg ccgccgacgc cggggatgtc
     4621 gatgcggccg agcgccgcag gtactcggcc gtcccccagg tgaacagcgc gaccgcgtcc
     4681 ttctcctcct ggtcgcgcgt gggctcgtgc atggtgacga cgatgagcgc gacggccccg
     4741 ggcttgcccg acccgacgag caccttgccg gagatgtcgg tcgcgcccgt cttgatgccg
     4801 tcgccccacg ggtagtcgag caggcggttg tggctctcca cggggacggc cgcgtccggc
     4861 gggtagcgga tgaccgccgt cttggtgcgc acgagctcgc ggaaggtcgc gtcctgcatg
     4921 gcgaaccgcc cgagggtcgc gaggtcgcgg gcggacgagt agtgaccgtc ctccggcgtg
     4981 ccgcggcagt tcaggaagtg ggtgtgcgtg aggcccagct gtgcggcgcg ctcgttcatg
     5041 agcttgacga accccttctc gctcccgccg acgtaggagg cgagggtgag cgccgcgtcg
     5101 ttggcgctct tgaccatcaa cgcctgcaga gcctcgcgga tgctgatgcg gtcgccgggc
     5161 acgaggtcca cgccgacgtt ctggggcagc gggatgtccg gcacggtcgc catcgcgtcg
     5221 aggtcgtcca cgacctccag ggtgagcagc gcggtcatga tcttggtgca ggaggcgggc
     5281 gccatctcgc ggtccgggtg cttgctccag agcacctcgc ccgagtcgcg cacgaggagg
     5341 atgccggtct gcgccttcac ctcgggcggc gcggtcccgg tcaccgcggg ctcggcgctg
     5401 gagggctcgt cgccgcaggc ggcgagcccc aggaccaggg cggcgacggc gagcgcgagg
     5461 acggcgagca gcgccgcggt gcgggcggat cgcgcgagtg ctgcgccgcc ggacgaacgg
     5521 gagctcactg tgcggcgggc cccgcgggtg tggcggcgag gaggtcgacg cggcccagga
     5581 cgagaccgtc cgagcggtac gtgaccgtgc ccaggacgtc gccgtcggcc ggcctcgcgg
     5641 gcagcggctc ctgcgggagg tcgaacacga gcgtcggcgc ggcggccttg cggatggtcg
     5701 ccgtcaagtc ggccccggcg acggcgtcga ccggggcgtc gccgtcgacc tgcagctgct
     5761 tgacgacctc acccgcggcg acgatctggc ggctctcgta cagcgccgag ccccacgtga
     5821 agagcgcgac cgcgtccctc tcctcctggt cgcgcgtggg ctcgcgcatg gtgacgacga
     5881 tcagggggac gagccccggc ttgcccgagc ccacgagcac tctcagcgac tgcttcgtgg
     5941 acccggtctt gatgccgtcg ccccaggggt agtcgagcag ccggttgtgc gaggtgacgg
     6001 tgacacggtg cgacggcggc caggtgatca cgcgcgtctt ggtgccgacg aggtcgcgga
     6061 accgtgcgtc tcgcatggcg tagcggccga gcctggcgag gtctcgcgcg ctggagtagt
     6121 ggccgggctt gggcgtgccg cgcgagttca cgaagcgcgt gtgggtgagg ccgagctgac
     6181 gggcgcgccg gttcatgagc ttgacgaagc ctgcctcgct gccgccgacg gccgtggcca
     6241 gcgtgacgca ggcgtcgttg gcgctcttgg tcatgagcgc gcgcagcgcc tgcttgacgc
     6301 tgatgcggtc gccgggacgc aggccgatgg cgacctgctg gaagtcccgc acgctgccgg
     6361 gcgcgctcac catgcgctcg aggttgctgt agcgctccag caccagcagc gcggtcatga
     6421 tcttggtgca cgaggccggc gccagccgca ggtccgcact cttggaccac agcacccggc
     6481 cggtgcgccg gtcgatgagg atcccgctgc gcgcccgcac gccgggcgcc gcctgggcgg
     6541 tcgtcgccgc gccctccgcc gagaccgact gcgcgtcggc cgctgcctgc gagggagcgc
     6601 gcgtcgcgac cgctgcgacg aaggcgccgg cagcgagcag gagcaggcac gccgccgcga
     6661 tgtacagtcg tgcgcggtgc tgtgtacgga tgcgtgacgc catgctccca gtatagcccg
     6721 gctcgttccg cgcggccgcg cctgcggtag actgccctcg ggccggcgta gctcagctgg
     6781 cagagcagcc gccttgtaag cggcaggtcg gcggttcgat tccgcccgcc ggctccatca
     6841 ggctcgacgt cgagcctcgc gaaggacagg taggccccgt ccgtattcca ggcgcgctcc
     6901 cggccacgtc gactacttcg cgagttcggc cgcccagcac cctgctcgag tcgacgcgcc
     6961 cctccgggcc ctcttcctgg tctcatcagc ggaggccgca ggcacgtcgc cgctgcccgt
     7021 cgagcggtag gggcgacacc tcggaagtcc gcggatgact gtccccgggg acagtccggc
     7081 agatgacgga ctgtcgctcc ggacattgtg caggcgcggg ccggatccta ggctctaggc
     7141 gtcagagcgg gcagagggat ggaggggaga gccatgcacg gcatgccgag gcacctgttc
     7201 gcggatgggt ggcgcttacg atcctttctg gtgatggcca tcgtcgtgat aggccttctg
     7261 ctgccggc
//
LOCUS       DASUMX010000002         4293 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1151674, whole genome shotgun sequence.
ACCESSION   DASUMX010000002 DASUMX010000000
VERSION     DASUMX010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4293
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1151674"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1862)
                     /locus_tag="VFZ86_00035"
     CDS             complement(<1..1862)
                     /locus_tag="VFZ86_00035"
                     /inference="COORDINATES: protein motif:HMM:NF019353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX5640708.1"
                     /translation="MSFVHLHTHTTFSFGDGACRIPEVVARAAGLGMPALAVTDHEGL
                     YGAVRFYQACKAAGIKPIVGVELTVEPAVGPGGRAWEPGADDGPPGDAAAIVADPAAA
                     AALAPDGRIAHLKASATVPPAAARASGAGGYHLVLLAKDYAGWSNLCRIISAAHLEHP
                     GEPPVARLATLAEHGGHLVALSGCRRGEVWSRLLAGDKAGARAAASVFHGIFGRDYFI
                     ELQHELLPDSGASLRALDLLAHELRLPTVATGDVHYTVKADAGLHDVLAAEAANQPLP
                     NPLGRKNAELYLKTPSQMRRLFQRYPEAYANAERIAARCNLDLGLGRLLFPAYPLPPG
                     ETAFSLLWKHCFEGAAERYRPLTAEVTARLERELKVIEELGFAEYFLAVRDIVEFALG
                     RGIYYSGRGSAGNSIVSYVLRITDADPIEHELLFERFLNPARREMPDVDIDFCSARRD
                     EVIQYIYERFGHDKVAMVATVNTVRAPSAVRIVSRAFGFTPDEINGLSRRVPWGSAAK
                     LGEMLAERPELAGHEFQQPHYHRLVDVAERLSGFPAHLGTHLGGFILSRDPLTDRVPL
                     QWAAKGVAVAQFDKDDVETLGLVKMDILALKMHSAIAEAVRRIETRTGEQVRAW"
     gene            complement(2155..2523)
                     /locus_tag="VFZ86_00040"
     CDS             complement(2155..2523)
                     /locus_tag="VFZ86_00040"
                     /inference="COORDINATES: protein motif:HMM:NF039800.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6504 family protein"
                     /protein_id="HEX5640709.1"
                     /translation="MPSGGATSGDRPEPPEAVAAGGRTERRTGAARARAGRPGPNGEA
                     VPIAVSWDERARRPVAFVWRRRRYQVERVLETWVLETGWWRDDGHVSRSYWRVRAGGR
                     VVDLRYDRLSKAWALEREIG"
     gene            2999..3985
                     /locus_tag="VFZ86_00045"
     CDS             2999..3985
                     /locus_tag="VFZ86_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017590279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEX5640710.1"
                     /translation="MYQRFATAIDELGRYGGLPTPIEAKQLWDDVWHLEAHHSTALEG
                     NTLVLREVEKLLEEGRAVGSKELKDYMEVLGYSEAARWVYEQGVAPKELVHEDLLSVT
                     EVRHVHELAMARVWDVAPHPQAIPDEVPGSFRRHDIHPFPGGMIPPTHTLVPAGVTSW
                     VDQVNRFGVDLADGTLSLTDAPEVLARIHVDFERLHPFIDGNGRTGRLLLNLVLTRLG
                     WPPIVIFREQRGRYLRALDRADHDDLGPLAELIVRAVVDNLHRLIPNIAGPAKYVPLE
                     ALADDEFSLAALKQAAGRGRLEAIIGNDGRYRSSRAALEAYKASRYKRGGQA"
BASE COUNT          641 a         1429 c         1564 g          659 t
ORIGIN      
        1 tcccaggcgc gcacctgctc gccggtgcgc gtctcgatgc gccgcaccgc ctcggcgatg
       61 gccgagtgca tcttgagcgc gaggatgtcc atcttgacga ggcccagcgt ctcgacgtcg
      121 tccttgtcga actgggccac cgcgacgccc ttggccgccc actgcagcgg cacgcggtcg
      181 gtgagcgggt cgcgcgagag gatgaacccg cccaggtgcg tgcccaggtg ggcggggaac
      241 cccgagagcc gctcggcgac gtcgaccagg cggtggtagt gcggctgctg gaactcgtgg
      301 ccggcgagct cggggcgctc ggcgagcatc tccccgagct tggccgcgct gccccagggg
      361 acgcgccggg agaggccgtt gatctcgtcc ggcgtgaacc cgaacgcacg gctgacgatg
      421 cgcaccgccg acggcgcgcg cacggtgttg acggtggcca ccatggcgac cttgtcgtgg
      481 ccgaagcgtt cgtagatgta ctggatgacc tcgtcgcggc gcgccgagca gaagtcgatg
      541 tccacgtccg gcatctcgcg gcgcgccggg ttgaggaagc gctcgaagag cagctcgtgc
      601 tcgatggggt cggcgtcggt gatgcgcagc acgtagctga cgatgctgtt gccggcggag
      661 ccgcggcccg agtagtagat accgcggccc agcgcgaact cgacgatgtc gcgcacggcg
      721 aggaagtact cggcgaaacc gagctcctcg atgaccttga gctcgcgctc gagccgcgcc
      781 gtgacctcgg cggtgagcgg ccggtagcgc tcggcggcgc cctcgaagca gtgcttccag
      841 agcagcgaga acgcggtctc gcccggcggc agcgggtagg cggggaagag gagcctgccc
      901 agccccaggt cgaggttgca gcgcgcggcg atgcgctccg cgttggcgta cgcctcgggg
      961 tagcgctgga acaggcggcg catctggctc ggggtcttga ggtagagctc cgcgttcttg
     1021 cgtcccagcg gattgggcag cggctggttg gcggcttcgg cggcgagcac gtcgtggagg
     1081 ccggcgtcgg ccttgaccgt gtagtggacg tcgccggtgg cgacggtggg gaggcgcagc
     1141 tcgtgggcga ggaggtcgag ggcgcgcagc gaggcgccgg agtccggcag cagctcgtgc
     1201 tgcagctcga tgaagtagtc gcggccgaag atgccgtgga acacagaggc cgcggctcgg
     1261 gcgccggcct tgtcgccggc gagcagccgc gaccagacct cgccgcgccg gcagccgctg
     1321 agcgcgacca ggtggccgcc gtgctcggcg agggtggcga gccgggccac cggaggctcg
     1381 cccggatgct cgaggtgggc ggcgctgatg atgcggcaga ggttgctcca gccggcgtag
     1441 tccttggcca ggagcaccag gtggtacccg ccggcgcctg aggcgcgggc ggcggcgggc
     1501 ggcaccgtgg ccgacgcctt gaggtgggcg atgcggccgt ccggcgcgag cgccgccgcg
     1561 gccgccgggt cggcgacgat cgccgcggcg tcgccgggcg gcccgtcgtc tgcccccggc
     1621 tcccaggccc ggccacccgg ccccaccgcc ggctcgaccg tgagctccac gccgacgatg
     1681 ggcttgatgc cggccgcctt gcacgcctgg tagaagcgca cggcgccgta cagcccctcg
     1741 tggtcggtga ccgccaaagc cggcatgccg agcccggcgg cgcgggcgac cacctcgggg
     1801 atgcggcagg cgccgtcgcc gaacgagaag gtggtgtgcg tgtgcaggtg gacgaagctc
     1861 atctcggcct ttcgggcgcg gtcatccgat ctcccgctcg agcgcccacg ccttggtaag
     1921 gcggtagtag cgcaggtcga cgacgtgacc gtcggcgctc ctgcacccgg gatcgcagac
     1981 ctcgagtgtg atacgaacat atgttcgtgt caacgtcgga cggaacgagc gaccggatcc
     2041 accttctctc ttcttccgag cgcggtgaag aaatgcgacc gttctccggt caatctgaag
     2101 tagcaggggt tgaggccggg gagcgcggcg gtcgcgcgcg tcggatgatc gcggtcagcc
     2161 gatctcccgc tcgagcgccc acgccttgct gaggcggtcg tagcgcaggt cgacgacgcg
     2221 gccgccggcg cgcacccgcc agtacgagcg gctgacgtgg ccgtcgtccc tccaccagcc
     2281 ggtctcgagg acccacgtct cgaggacgcg ctcgacctgg tagcggcggc ggcgccagac
     2341 gaaagcgacc gggcgccgcg ctcgctcgtc ccagctcaca gcgatcggca cggcctcgcc
     2401 gttggggccc gggcggccgg cgcgggcccg ggcggcaccg gtgcggcgct ccgtccggcc
     2461 gccggccgcc accgcttcgg ggggctcggg tctgtcgccg gaggtggctc cgccgctcgg
     2521 catgtggggc tcctgacgcg cggatggacg aggctgcggg ggcattgagc gtagcacgaa
     2581 catatgttcg tgtaaacgtc ggacggaaga gtggacttga gggcccggct tcattgtctc
     2641 cccgctcatc cttgccgctc cgccggcgcc cgcatagcct tggtcagctc tttggtgtct
     2701 gcgttgacct tgagagggga caccgccatg gtggctgctt ctcaaacgga tcgtctgctt
     2761 cttggcggcg tgagcccggc agtgaccgtt cccgggctgc ggcgcgccgg tttcggtgcg
     2821 cagcaaaatg gcctccgagc gaaaatggga atggccgcat aatcgaagtt gtcattgtgg
     2881 aattacctgg cgtctgcgac gacaatttgc taagttgtcg ttgttgattt actgagggga
     2941 tgcgatgaca acttctccga ccgggcgtgc tccccgaggg cggcccccac gaatcgcggt
     3001 gtaccagcgc ttcgccaccg cgatcgacga gctcgggcgc tacggcgggc tccccacgcc
     3061 gatcgaagcg aagcaactct gggatgacgt gtggcacctc gaggcccacc actcgacggc
     3121 gcttgagggc aacacgcttg tgcttcgcga ggtcgagaag ctcctcgagg agggccgcgc
     3181 cgtcggctcg aaggagctca aggactacat ggaggtcctc ggctattcgg aggccgcgcg
     3241 ctgggtctac gagcagggag tagccccaaa ggaactcgtg catgaggatc tcctcagcgt
     3301 gaccgaggtc cgccacgttc acgagctggc gatggcaagg gtgtgggacg tggcgccaca
     3361 tcctcaggca atcccggacg aggtgcccgg cagcttccgg cggcacgata tccatccgtt
     3421 tccgggagga atgattccgc cgactcacac actcgtcccg gcgggcgtca cctcctgggt
     3481 cgatcaggtg aatcggttcg gcgtcgatct cgccgacggc acgctcagtc tcactgatgc
     3541 gccggaggtg ctcgcccgca tccacgtcga cttcgagcgg ctgcacccat tcatcgacgg
     3601 caacggccga accggacggc tcctgctgaa ccttgtgctg actcgtctcg gatggccacc
     3661 gatcgtcatc ttcagagaac agcgcggacg ctacctcaga gcgctcgacc gagcggatca
     3721 cgatgacctt ggtccactgg cggagctcat cgtccgcgcc gtcgtcgaca accttcacag
     3781 gctcatcccc aacatcgcgg gaccggcgaa gtacgtgccg cttgaggccc tcgccgacga
     3841 cgagttcagc ctcgcggcgc tcaagcaggc ggccgggcgc ggccggctcg aagcgatcat
     3901 cggcaacgac gggcgatacc gatccagccg cgccgcactt gaggcgtaca aggcgtctcg
     3961 ctacaagcgc ggcggccagg cgtgagcgac atcccgagcc gcgttcgttg cgacccccat
     4021 cgtcgtgaac tcggagagca cggtcgcgcc gagtacctct gagaccgcgg acgaggcggc
     4081 gtaccaggcc gaggccgctc tggagcagac aagaacgtgc tgccgttccg catcgccttc
     4141 gcgaagaagc agcgcgtgct ggcgaagctc ggcgagttct tcgagcggtt cttcgggctc
     4201 ggcacgagct gaggcgcggc cgcggcgcct caggcgccgg cgttcgcgtc aggcggtgac
     4261 gctcacctcg gcgaggtcgt cgacgaccac gtc
//
LOCUS       DASUMX010000003         9962 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1296036, whole genome shotgun sequence.
ACCESSION   DASUMX010000003 DASUMX010000000
VERSION     DASUMX010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 9962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9962
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1296036"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1637)
                     /locus_tag="VFZ86_00050"
     CDS             complement(<1..1637)
                     /locus_tag="VFZ86_00050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEX5640711.1"
                     /translation="MTDATDRSTKLSGTTFSRVVRAGALAVVREQETLNRINVFPVRD
                     ADTGANLAATLRSAASRLGNEAPDSVGVAARVAADGALDGARGNSGAIFAQFLHGLAS
                     SMERLRSADGSQFADAAQAGTESAYSALQEPREGTILSVLRAWSHELGSRARDHEDDL
                     PELLRHGLVAAKDALAATPHQLEVLARSKVVDAGGQGFVFFLEGLLDSLRGGHEPAWE
                     ACEAPAHGLPPFSDPHDEIDERFRFCTEALLRARDDEQFSRDLVMARVTGLGDSLVVA
                     GGGSRLRVHLHTNEPQRFLASVAELGVIEHSKVDDMVLQQLAGREAAIAIVTDSTIDL
                     PEEQAFRLGVVTVPLTLSLGEAEFLDGVDISAEGFVHRILSGSDVPRSSQPAVADFVQ
                     TYRRLLEYREGVVSVHIAGAMSGTVQAARAAAREVDPTRVRVVDSCLVSIGAGLLTEA
                     IGEAIAAGAELDEVERVAERVKREIRIFGTVASLDFAVRGGRVSPTMARTLDRLHLAP
                     IIVFDEHGKPGKGGAALGFDRALNAIVKRAVSFADGAP"
     gene            complement(1675..2181)
                     /locus_tag="VFZ86_00055"
     CDS             complement(1675..2181)
                     /locus_tag="VFZ86_00055"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX5640712.1"
                     /translation="MALFGPPNVAKLDAQGKIDGLVKAARYKDPEVAAAARGALEGYL
                     DKLIDRLQTKNIVQLDTTRDALLIIGPPARDRLIFILREGHLHRRQDAAYVLGLMKDP
                     VAVKPLCLAMHNPDPLLRVIIVEALGRIGDPAATDTLRRALGDVGKAVAGAARKALKQ
                     IGALPSGT"
     gene            complement(2250..3137)
                     /locus_tag="VFZ86_00060"
     CDS             complement(2250..3137)
                     /locus_tag="VFZ86_00060"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HEX5640713.1"
                     /translation="MEVRGLPSCAPEPPAGDAPLAALATRYRVDQEDLDGWTIAVNLT
                     RSDCRLADAGLRYYAERLAARAVLQRAARLVGPLQAATQTVREPQREPWAGELDVEAT
                     LDNVLGTPHPEPGDWIAQRRVDRRHQVVLMVDTSLSMSGENMAIAAVAAAVLALKLHP
                     EDLSVVVFEDKATAVTHLDVPDAPHEVVRRMLDQPVRGYTNVEAALGLGAAELERGRN
                     PRRSGLLITDGVVTAGGDPRPLAHRFPQLFVLLTEDYKMNPELCRELADAGHGDVFPV
                     RAFHDLPARLLDVANRILR"
     gene            complement(3176..4105)
                     /locus_tag="VFZ86_00065"
     CDS             complement(3176..4105)
                     /locus_tag="VFZ86_00065"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX5640714.1"
                     /translation="MTKVEEMTPIEQIQQQHGIIGRERELATALAVLGAGRHLLLEGP
                     VGVGKTTVALAVCGHLGRDTVRVDGDDRYSESKLTGWFDPPLVLKQGYGEEAFFAGPL
                     VEAMREGKVLFINELNRMPESVQNVLLPALDERLLQVPHIGEVRASAGFQVVATQNPV
                     EYVATGHLSEALRDRFEHLALDYQDAAEEAGIVAAETGSDDAALVQTAVRLTRATRLH
                     PRFRKGASVRGAIATVAIAERLWADDREACGAVSREVLRRAAEAALTTRVDLRDDIDA
                     DFGAALDELLELVVERGEDPDVALAPPGSSPKA"
     gene            complement(4236..6083)
                     /locus_tag="VFZ86_00070"
     CDS             complement(4236..6083)
                     /locus_tag="VFZ86_00070"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5640715.1"
                     /translation="MDAVGEMQRRYGVEGRERELETALAALAGGRHLLLEGPVGAGKT
                     TVARAVCGHLRRETVRVDGDDRCTESRLAGWFDPPLVLRLGYRDEAFVAGPLVRALRR
                     GHVLFLNELNRLPEAAQNLLLPALDEGVLQVPHLGEVRAAEGFQVVATQNPAEYVAVG
                     HLSEAVRDRFEHLEIGYQTAAEEEAIVVAATGCRDAGLVRAAVRLVRATRLHPRVRRG
                     ASVRGAIAIVELTCALDDHGPSGAAAGGGLRRAAAAALANRVELRDGDGRLDALLDEL
                     LALIVGRGEDPEAGEAAPAEGGRADARGRREGARPPADPAEPESCTLEDRRALAAQLR
                     ADPEDLDGWRLAADLSSGVLTHAGERAYLCARQLAAAAVLRRAARLVGPLRASTATVR
                     EPQREPWAGELDVEATLDNVLGKPHPEPGDWIAQRRVDRRHQVVLMVDTSLSMAGEKM
                     ALAAVAGAVLALKLRPGDLAVVLFADGARVASRFGEELAPAELVRRMLSVPCGGGTDI
                     AAALESGHAELRRGRDPSRCGLLVSDGVCTSGADPLPAAARFGPLHVLLMEERVADRD
                     PTAPVTTWIAPRLRAGEAIARAAGGGLVRVDGFPALPRRMLDVADRVLR"
     gene            6124..6897
                     /locus_tag="VFZ86_00075"
     CDS             6124..6897
                     /locus_tag="VFZ86_00075"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEX5640716.1"
                     /translation="MALPPDIGLPSDAWYGLPLGAALERVAKYASLAEIASTEEHDLL
                     ATANRRAAVESGLRLTVHGPWDDLEPGSTRERDRRHALAVHRRHLEAAAEIGAETYVV
                     HPDYQGCAVPRDPRIVDALRRTFAELEEYQRELGVRIVVENLPGVGFSHFGAPGDLDL
                     GELGLILDTGHAAICGTLHDFLSAPHAELAHVHLHDNRGPADAGDPHLPLGRGVVDAR
                     AVLRAARASGATVILEVLDEAAIRESIAHLERKGLLDTG"
     gene            complement(6944..8044)
                     /locus_tag="VFZ86_00080"
     CDS             complement(6944..8044)
                     /locus_tag="VFZ86_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018257423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5640717.1"
                     /translation="MEASVSSDTMGATYARLAGLGLEIERYRTERHELDVSSGFRRVT
                     TEIVLVGGGLEGRGEDVTYAAEDHDGYPAELPLAGEWTLDAFSGRLGGLDLFPGRPPQ
                     EESFRDYRRWGFESAALDLALRQARQNLGEALGRPFAPVRFVLSTRLDIKPWLAVDPG
                     LEFKLDPTPEWDAGLMRDIAATERVRALDFKSFYEGSPVDNPPDAVQYRLVSETFPGT
                     VLEDPALEGPAREALRGQEARFSFDAPIHSWADVEMVTSRVATACGPECDTFTLRQLN
                     IKPSRFGSLRALLDCVGRAQAAGMVLYGGGQFELGIGRDQIQALASLLYPDGPNDVAP
                     REYHGAPHADVPPSPLVPLGHQGPGFGFSFEV"
     gene            8144..8908
                     /locus_tag="VFZ86_00085"
     CDS             8144..8908
                     /locus_tag="VFZ86_00085"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEX5640718.1"
                     /translation="MQRPHDIGISTSAYAEHLLPAALERISEHASFAEIRSFGLHTLR
                     STRNRAEARRAGLTYSVHGPFGYTGIWDLDEAERLKALDEHRTHLEASAEIGAFLYVV
                     HPDWRPGVEPRDPAVVAQLGRSFETLRDWQDELGVEIVVENMPGAGMSHFTHPGDLDL
                     RGLGLILDVGHASISGCLAEWLDDPRAPLRHLHLHDNHGEGDGDDPHLGLGAGVVDAA
                     AVMAAARAAGATAVLEHASDDAVRISLARLRELGLL"
     gene            complement(8852..9385)
                     /locus_tag="VFZ86_00090"
     CDS             complement(8852..9385)
                     /locus_tag="VFZ86_00090"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX5640719.1"
                     /translation="MTPPASDLTPEATLRRCDLLRPLSERQIAELAACATTRSLRAGE
                     PLFDQNDEANDFFVVAKGRVVVRMTAPGGGAIEVFDAGEFRLCGWSSLVAPHEYVAGA
                     TAAEDSTVLVVPAAAAEDIFLLEPFAGYEVMQRLAGDISVRLRNVRTELLELLNGAGS
                     EQAELAQAGEADPHGVV"
     gene            complement(9436..9909)
                     /locus_tag="VFZ86_00095"
     CDS             complement(9436..9909)
                     /locus_tag="VFZ86_00095"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX5640720.1"
                     /translation="MRPPTADLSVESTLAHCELLEPLSEAQIAALSDHCTMVVLREGE
                     ALFDQNTVAHSLFVVAGGRLAVRLATPAGRVIEVVEVGQYGLSGWSSIVAPHIYVADA
                     KALEDTTVIAISADEVEDILLREPAAGYEVMKKLAGLISTRLRDIKEELIEVLES"
BASE COUNT         1307 a         3757 c         3603 g         1295 t
ORIGIN      
        1 ggcgcgccgt ccgcgaagct cacggcgcgc ttcacgatcg cgttcagggc gcggtcgaac
       61 ccgagcgccg cgcccccttt gcccggcttg ccgtgctcgt cgaagacgat gatcggcgcg
      121 aggtgaagcc ggtcgagcgt cctcgccatc gtcgggctca cgcgcccgcc gcgcacggcg
      181 aagtcgagcg aggcgacggt gccgaagatc ctgatctccc gcttgacccg ctcggccacg
      241 cgctccacct catcgagctc ggcgccggcg gcgatggcct cgccgatggc ctccgtgagc
      301 aggccggcgc cgatggacac gaggcacgag tcgaccaccc gtacgcgcgt gggatcgacc
      361 tcgcgtgccg ccgcccgcgc cgcctggacc gtgccggaca tcgccccggc gatgtggacg
      421 ctcaccacac cctcgcggta ctcgagcagc cggcggtagg tctggacgaa gtcggccacc
      481 gccggctgtg acgagcgcgg cacgtccgac ccgctcagga tgcggtgcac gaacccctcg
      541 gccgagatgt cgaccccgtc gaggaactcg gcctcgccca gcgagagcgt gagcggcacg
      601 gtcaccaccc cgaggcggaa cgcctgctcc tcgggcaggt cgatggtcga gtcggtgacg
      661 atggcgatgg cggcctcgcg gccggcgagc tgctgcagca ccatgtcgtc gaccttgctg
      721 tgctcgatca cgccgagctc cgccaccgag gcgagaaacc gctgcggctc gttggtgtgc
      781 aggtggaccc gcagccgcga accgccgccc gcgaccacca gcgaatcccc gagcccggtg
      841 acccgcgcca tgaccagatc gcgggagaac tgctcgtcgt cgcgcgcccg gagcagcgcc
      901 tccgtgcaga agcggaagcg ctcgtcgatc tcgtcgtgcg ggtccgagaa cggcggcagc
      961 ccgtgcgccg gggcctcgca ggcctcccac gcgggctcgt ggccgccgcg cagcgagtcc
     1021 aggaggccct cgaggaagaa gacgaagccc tggccgccgg cgtcgacgac cttgctgcgc
     1081 gccaggacct cgagctggtg cggcgtcgcc gcgagcgcgt ccttggcggc cacgaggccg
     1141 tggcgcaaca gctcgggcag gtcgtcctcg tggtcgcggg cacggctgcc cagctcgtgg
     1201 ctccaggcgc ggagcacgct gaggatcgtg ccctcgcgcg gctcctgcag ggccgagtac
     1261 gcggactccg tgcccgcctg cgccgcgtcg gcgaactgcg acccgtcggc gctgcgcagg
     1321 cgctccatgg aggaggcgag cccgtgcagg aactgggcga agatcgcccc ggagttgccg
     1381 cgggccccgt cgagggcgcc gtcggcggcg acacgggcgg cgacacccac cgagtcggga
     1441 gcttcgttcc cgagcctgga agccgccgac ctgagcgtcg ccgccaggtt ggcgcccgta
     1501 tcggcatcgc gcaccgggaa gacgttgatg cggttcaggg tctcctgctc gcgcacgacg
     1561 gcgagcgcac cggcgcgcac gacgcgcgag aacgtcgttc cgctcagctt cgtcgatcgg
     1621 tcggtcgcgt cggtcatctc tgcagcatgc tacccgttcg agccggaggg tcggtcacgt
     1681 ccccgaggga agcgcgccga tctgtttgag cgccttgcgc gccgcgccgg cgaccgcctt
     1741 gcccacgtcg cccagggcgc ggcggagcgt gtccgtcgcc gcggggtcac cgatccttcc
     1801 cagcgcctcg acgatgatca cgcgcaggag cgggtcaggg ttgtgcatcg cgagacagag
     1861 aggcttgacc gcgaccgggt ccttcatcag accgagcacg taggccgcgt cctgccgccg
     1921 gtggaggtgg ccctccctga ggatgaagat caaccggtcg cgagcgggcg ggccgatgat
     1981 gagcagcgcg tcgcgggtgg tgtccagctg gacgatgttc ttggtctgca gccggtcgat
     2041 gagcttgtcc aggtagcctt cgagagctcc gcgcgcggcc gcggcgacct cggggtcctt
     2101 gtacctggcg gccttgacga ggccgtcgat cttgccttgc gcgtcgagct tggcgacgtt
     2161 gggcgggccg aacagggcca cggagcactc ctcagggtcg gaccttgcgg gcaggtcgcg
     2221 ggcgctcctg gggcgcccgg gaggcaagtc taccgcagga tgcggttggc gacgtcgaga
     2281 aggcgcgccg ggaggtcgtg gaaggcgcgc accgggaaga cgtcgccgtg gccggcgtcg
     2341 gccagctcgc ggcagagctc cgggttcatc ttgtagtcct cggtgagcag gacgaagagc
     2401 tgcgggaagc ggtgcgccag cggccgtggg tcgccgcctg ccgtcacgac gccgtcggtg
     2461 atcaggaggc cgctgcggcg tgggttgcgg ccgcgctcca gctcggcggc gccgagcccc
     2521 agcgcggcct cgacgttggt gtagccgcgc accggctggt cgagcatgcg ccgcacgacc
     2581 tcgtgcggcg cgtccggcac gtccaggtgc gtcacggccg tcgccttgtc ctcgaagacc
     2641 acgacggaga ggtcctcggg gtgcagcttg agcgccagga ccgccgcggc cacggcggcg
     2701 atcgccatgt tctcgccgct catgctgagc gaggtgtcga ccatgagcac cacctggtgg
     2761 cggcggtcga cgcgacgctg cgcgatccag tcgccgggct ccgggtgcgg cgtgccgagc
     2821 acgttgtcga gcgtcgcctc cacgtcgagc tcgccggccc agggctcgcg ctgaggctcg
     2881 cggacggtct gggtcgcggc ctgcagcggg ccgacgaggc gggcggcgcg ctggagcacg
     2941 gcccgcgccg ccagccgctc ggcgtagtac ctcaggcccg cgtcggcgag gcggcagtcg
     3001 ctgcgcgtca gattgacggc gatcgtccag ccgtcgaggt cctcctggtc gacgcggtag
     3061 cgcgtggcca gcgccgccag gggcgcgtcg ccggcaggcg gctccggggc gcacgacggc
     3121 aggccgcgga cttccacgcg gccctggtgc cggagcggcg ccccggcgga tggcgtcagg
     3181 cttttgggct tgaccccggc ggcgcgagcg cgacgtccgg gtcctcgccg cgctcgacga
     3241 cgagctcgag cagctcgtcg agcgcggcgc cgaagtccgc gtcgatgtcg tcgcgcaggt
     3301 cgacgcgggt ggtcagcgcg gcttcggccg cgcggcgcag cacctcgcgg ctcacggccc
     3361 cgcaggcttc gcggtcgtcg gcccacagcc gctcggcgat ggcgaccgtc gcgatcgccc
     3421 cgcgcaccga ggcgcccttg cggaagcgcg ggtgcagccg cgtcgcccgc gtgagccgca
     3481 cggccgtctg caccagcgcg gcgtcgtccg agccggtctc cgcggcgacg atccccgcct
     3541 cctccgccgc gtcttgatag tccagagcca ggtgctcgaa gcggtcgcga agcgcctccg
     3601 agaggtgccc cgtggcgacg tactccaccg ggttctgcgt cgccaccacc tggaaccctg
     3661 cgctcgcccg cacctccccg atgtgcggca cctgcagcag ccgctcgtcc agcgccggca
     3721 ggagcacgtt ctgcacgctc tcgggcatcc ggttcagctc gttgatgaac agcaccttgc
     3781 cctcgcgcat ggcctcgacc agcggccctg cgaagaacgc ctcctccccg tacccctgct
     3841 tgagcacgag cggcgggtcg aaccaccccg tgagcttgct ctccgagtag cggtcgtcgc
     3901 cgtccacgcg cacggtgtcg cgcccgaggt ggccgcagac ggccagggcg acggtggtct
     3961 tgcccacgcc caccgggccc tcgagcagca ggtggcggcc ggcgcccagc acggccagcg
     4021 ccgtggccag ctcgcgctcg cgtccgatga tgccgtgctg ttgctggatc tgctcgatgg
     4081 gagtcatctc ttcaaccttc gtcatcgcgc tgccgcagct ccctgccgca gactgggtat
     4141 ctgatctcct cgacgatatc aggcccggag gcgcttgggc gcgtccggcc ctggcggagt
     4201 acccgcgcct ccgcgccgct ccggcctcgg atcgctcagc gcagcacccg gtcggccaca
     4261 tccagcatgc gccgcgggag cgccgggaag ccgtcgacgc gcacgaggcc gccgccggcc
     4321 gcgcgggcga tggcctcgcc ggcgcggagc cggggcgcga tccaggtggt caccggcgcc
     4381 gtggggtcgc gatccgccac ccgttcctcc atcagcagca cgtgcagcgg cccgaaccgc
     4441 gccgccgccg gcagcgggtc ggcgccgctc gtgcagacgc cgtcgctcac gagcaggccg
     4501 cagcggctcg ggtcgcggcc gcgccggagc tccgcgtgcc cggactcgag cgcggcggcg
     4561 atgtccgtgc cgccgccgca cggcacggag agcatgcgac ggaccagttc cgccggcgcc
     4621 agttcctcgc cgaagcgcga ggccacgcgc gcgccgtcgg cgaacagcac gacggcgagg
     4681 tcgccgggcc gcagcttgag cgccagcacg gcgccggcca ccgcggcgag cgccatcttc
     4741 tcccccgcca tgctgagcga ggtgtcgacc atgagcacca cctggtggcg gcggtcgacg
     4801 cggcgctgcg cgatccagtc gcccggctcc gggtgcggct tgccgagcac gttgtcgagc
     4861 gtcgcctcca cgtcgagctc gccggcccag ggctcgcgct ggggctcgcg gacggtcgcc
     4921 gtcgacgccc tgagcgggcc gacgaggcgg gcggcgcggc gcagcacggc ggcggccgcg
     4981 agctgccggg cgcacaggta ggcgcgctcg ccggcgtgcg tgagcactcc cgagctgaga
     5041 tcggcggcga gccgccatcc atcgagatcc tcggggtccg cgcgcagctg cgccgccagc
     5101 gcgcggcgat cctcgagcgt gcagctctcc ggctcggccg gatccgccgg cggccgcgct
     5161 ccctcccgcc ggccgcgtgc gtcggcccgg ccgccctccg cgggcgcggc ctcgccggcc
     5221 tcggggtcct cccccctccc gacgatgagc gccagcagtt cgtcgagcag cgcgtccagc
     5281 cgcccgtccc cgtcgcgcag ctccacgcgg ttcgcgagcg ccgcggcggc ggcgcggcgc
     5341 aggccgccgc ctgcagctgc gccggacggc ccatggtcgt ccagcgcgca cgtcagttcc
     5401 acgatcgcga tggcgccgcg caccgacgcc ccgcggcgca cgcgcgggtg cagccgcgtc
     5461 gcccggacga gcctcacggc cgcgcgcacc agtccggcgt cccggcaacc ggtcgccgcc
     5521 acgacgatgg cctcctcctc cgcggcggtc tggtagccga tctcgaggtg ctcgaaccgg
     5581 tcgcggacgg cctcggacag gtggccgacg gccacgtact ccgccgggtt ctgcgtcgcc
     5641 accacctgga agccctcggc ggcgcgcacc tcgccgaggt gcggcacctg cagcacgccc
     5701 tcgtcgagcg ccggtagcag caggttctgc gccgcctccg gcaggcggtt cagctcgttg
     5761 aggaagagga cgtggccgcg gcgcagtgcc cggaccagcg gcccggcgac gaaggcctcg
     5821 tcgcggtagc cgagccgcag caccagcggg gggtcgaacc agcccgcgag gcgactctcc
     5881 gtgcagcggt cgtcgccgtc cacacggacg gtctcgcgcc gcaggtggcc gcagacggcg
     5941 cgggcgacgg tcgtcttccc cgcgcccacg ggtccctcga gcagcaggtg acgcccgccc
     6001 gcgagcgcgg cgagcgccgt ctcgagctca cgctcgcgtc cctcgacgcc gtaccgccgc
     6061 tgcatctccc ccaccgcgtc caccacccga tgctaccgca ccgcgatggg tagacttgcg
     6121 gccatggcgc tcccgcccga catcggcctc cccagcgacg cgtggtacgg cctccccctc
     6181 ggcgccgcgc tcgagcgggt ggcgaagtac gcatcgctcg ccgagatcgc ctccacggag
     6241 gagcacgacc tgctggcaac ggccaaccgg cgggcggccg ttgagagcgg gctccgcctc
     6301 accgtccacg gtccctggga cgatctggaa ccgggcagca cgcgcgagcg agaccggagg
     6361 cacgcgctcg ccgtgcaccg ccgtcacctg gaggcggcag cggagatcgg cgccgagacc
     6421 tacgtggtgc accccgacta ccagggctgc gcggtgccgc gcgacccgcg gatcgtggac
     6481 gcgctgcggc ggacgttcgc cgagctcgag gagtaccagc gcgagctcgg cgtccgcatc
     6541 gtcgtcgaga acctgccggg cgtcggcttc tcgcacttcg gcgcgcccgg cgacctcgac
     6601 ctcggcgagc tcggactgat cctcgacacg gggcacgcgg cgatctgcgg gacgctgcac
     6661 gatttcctga gcgcgccgca cgcagaactg gcgcacgtcc acctgcacga caaccgcggc
     6721 ccggcggacg ccggcgaccc gcaccttccg ctgggccggg gcgtcgtcga cgcgcgcgcg
     6781 gtgctgcgcg cggcacgcgc gagcggcgcc accgtcatcc tcgaggtgct ggacgaggcg
     6841 gcgatccgcg agagcatcgc gcacctggag cggaagggcc tgctggacac cggctgagcc
     6901 gggacgccgg gcgcgcgggc cggctgcgcc gccgcgcccg ccttcacacc tcgaacgaga
     6961 acccgaaccc gggcccctgg tgccccagcg gcacgagcgg gctcgggggc acgtccgcgt
     7021 gcggcgcgcc gtggtactcg cgcggggcga catcgttggg gccgtccggg tacaggagac
     7081 tggccagcgc ctggatctgg tcgcggccga tgccgagctc gaactggccg ccgccgtaca
     7141 ggaccatgcc ggcggcctgc gcgcgcccga cgcagtcgag cagcgcgcgc aggctcccga
     7201 agcgcgacgg cttgatgttg agctggcgaa gcgtgaacgt gtcgcactcc gggccgcagg
     7261 ccgtggcgac cctcgacgtg accatctcca cgtccgccca cgagtggatc ggcgcatcga
     7321 agctgaagcg cgcctcctgg ccccggagcg cctcacgcgc cgggccctcc agcgccggat
     7381 cctcgagcac cgtgccgggg aacgtctcgc tcacgagccg gtactgcacg gcgtccggcg
     7441 gattgtcgac cggcgagccc tcgtagaacg acttgaagtc gagggcgcgc acgcgctccg
     7501 tcgcggcgat gtcccgcatg aggccggcgt cccactccgg cgtggggtcg agcttgaact
     7561 cgagacccgg atcgacggcg agccagggct tgatgtcgag gcgcgtgctg agcacgaagc
     7621 gcacgggggc gaacggccgg ccgagcgcct cgcccaggtt ctgccgcgcc tgccgcaggg
     7681 ccaggtcgag ggcggcgctc tcgaagcccc agcgccggta gtcgcggaag gactcctcct
     7741 ggggcggcct gccggggaac aggtccaggc cgcccaggcg ccctgagaac gcgtcgagcg
     7801 tccactctcc ggcaagagga agctccgccg ggtagccgtc gtggtcctcg gccgcgtagg
     7861 tgacgtcctc gccgcggcct tccagcccgc cgccgacgag cacgatctcc gtggtcacgc
     7921 ggcggaagcc gctggagacg tccagctcgt gacgctcggt ccggtaccgc tcgatctcca
     7981 ggccgagccc ggcgagccgc gcgtaggtcg cgcccatggt gtcgctgctg accgaggcct
     8041 ccatgcgccg cttctacccg ccgggtccac aaggcaaccg ggcgccgccg gtggcaggcc
     8101 ggccgcacgc accgggcgcg cggctctggc atactgggac cccgtgcagc gcccccacga
     8161 catcggcatc tcgacgagcg cctacgcgga gcacctgctc ccggcggcgc tggagcgcat
     8221 ctccgagcac gcctccttcg ccgagatccg ctccttcggc ctgcacacgc tgcgcagcac
     8281 gcggaaccgc gccgaggcgc gccgcgcggg cctcacgtac tccgtgcacg gcccgttcgg
     8341 ctacacgggc atctgggacc tcgacgaggc cgagcgtctc aaggcgctcg acgagcaccg
     8401 cacgcacctg gaggcgagcg ccgagatcgg ggcgttcctc tacgtcgtgc accccgactg
     8461 gcgcccgggg gtcgagcctc gcgaccccgc cgtcgtcgcg cagctgggcc ggtccttcga
     8521 gacgctgcgc gactggcagg acgagctcgg cgtggagatc gtcgtcgaga acatgccggg
     8581 cgccgggatg tcccacttca cccaccccgg cgacctcgac ctgcgcggcc tggggctcat
     8641 cctcgacgtg ggacacgcca gcatcagcgg ctgcctcgcg gagtggctcg acgacccgcg
     8701 ggcgccgctg cgccacctgc acctgcacga caaccacggc gagggcgacg gggacgaccc
     8761 gcacttgggg ctcggcgccg gcgtcgtgga cgcagcggcc gtgatggccg cggcgcgagc
     8821 cgccggcgcc acggccgtcc tcgagcacgc ctcagacgac gccgtgcgga tcagcctcgc
     8881 ccgcctgcgc gagctcggcc tgctctgacc ctgccccgtt gagcagctcg agcagctccg
     8941 tcctcacgtt gcgcagacgg accgagatgt cgccggccag acgctgcatc acctcgtagc
     9001 cggcgaaggg ctcgaggagg aagatgtcct cggccgccgc cgcaggcacc accagcacgg
     9061 tcgaatcctc ggcggcggtc gccccggcca cgtactcgtg cggggcgacc agcgacgacc
     9121 acccgcagag gcggaactcc cccgcgtcga acacctcgat cgcaccgccg ccgggcgccg
     9181 tcatgcgcac gacgacccgc cccttcgcga ccacgaagaa gtcgttcgcc tcgtcgttct
     9241 ggtcgaagag cggctcgccc gcgcgcagcg agcgcgtggt cgcgcacgcg gccagctcgg
     9301 cgatctgccg ctcgctcagc ggccggagca ggtcgcagcg gcgcagcgtg gcctcgggcg
     9361 tgagatcgga agcgggcggt gtcatctggt ccccctcatg atcggtgagc gcgacggcgg
     9421 cacgacggga cggtctcagc tctccagcac ctcgatcagc tcctccttga tgtcgcgcag
     9481 gcgcgtcgag atgaggccgg cgagcttctt catcacctcg tagccggccg ccggctcgcg
     9541 cagcaggatg tcctccacct cgtctgcgga gatggcgatg acggtggtgt cctcgagcgc
     9601 cttcgcgtcc gccacgtaga tgtgcggcgc cacgatcgac gaccagccgg acaagccgta
     9661 ctggccgacc tcgaccacct cgatgacgcg gccggcagga gtcgccaggc ggaccgccag
     9721 acgcccgccg gccacgacga acaggctgtg cgccacggtg ttctggtcga acagggcttc
     9781 gccctcgcgc agcacgacca tggtgcagtg atctgagagc gcggcgatct gagcctcgct
     9841 caggggctcc agcagctcgc agtgcgcgag cgtgctctcc acggacaggt cggcggtcgg
     9901 tggcctcatg tcgctccccc ctctggtaga catggcacat cgtgtgtcta cccgttcggc
     9961 gg
//
LOCUS       DASUMX010000004         4267 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1583773, whole genome shotgun sequence.
ACCESSION   DASUMX010000004 DASUMX010000000
VERSION     DASUMX010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4267
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1583773"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /locus_tag="VFZ86_00100"
     CDS             <1..688
                     /locus_tag="VFZ86_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HEX5640721.1"
                     /translation="VAFRETVNVPLVIVDPRHPGGGRTDAVGSHLDLVPTILAYAGLP
                     EAERQQRYPFLKGHDLSGIVEDPASAGPRGASGEPGKGCLLTYDMIATVDAEWFSRNA
                     TRVFDAAAAQAGLEFHRGMDSFKGMLDEIGVPDLEKRELFRCVFDGRYKLVRYFGLGG
                     YNLPASVKQLLADNDAALYDLLLDPEEMDNLADPANPKYSEELLSAMNQKLNALIDEE
                     IGEDKALFTP"
     gene            complement(807..882)
                     /locus_tag="VFZ86_00105"
     tRNA            complement(807..882)
                     /locus_tag="VFZ86_00105"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(846..848),aa:Glu,seq:ttc)
     gene            complement(942..1409)
                     /locus_tag="VFZ86_00110"
     CDS             complement(942..1409)
                     /locus_tag="VFZ86_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5640722.1"
                     /translation="MKITAVRANNRKRTFEVTTRRGAWPFPYSKADPPPSTSDPVERL
                     FVDPELGGEGFTYELASGTDGCVLMDQVLDYNEEPGYMRDLLLHNLTCEALDRLEASP
                     LSKREIIRRLGTSPAQFYRLLDTTNYRKSVDKMLSLLQVLGCEVEVIVREKSA"
     gene            complement(1406..1600)
                     /locus_tag="VFZ86_00115"
     CDS             complement(1406..1600)
                     /locus_tag="VFZ86_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5640723.1"
                     /translation="MTRVRRGGYVFISWVGDHSPRHVHVLRDGRLVLKWNLDRNRPMA
                     GVPTARLVALIRELESEGLL"
     gene            1771..2196
                     /locus_tag="VFZ86_00120"
     CDS             1771..2196
                     /locus_tag="VFZ86_00120"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX5640724.1"
                     /translation="MSTTDQTRRRFTTPVRVRYADTDATGTLHPATYLMYFEVARVEA
                     LRGLGVPVGEFATRGVAMPVIEAGVNVVRPARIDDLLEVDVLLDRIGPASFSFEYEVA
                     RDGLLLATGFTRMAVIDPADGRALRLPDWVRDLLEGIGS"
     gene            2324..4036
                     /locus_tag="VFZ86_00125"
     CDS             2324..4036
                     /locus_tag="VFZ86_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HEX5640725.1"
                     /translation="MILESPERIAEYTARGWWGDKTLADLFLENVRRTPEAVAVVDPP
                     NRAALTPGEPQRLTYGELKTAADRLAKALLDAGVGRDDVVMVQLPNIVELVVVYLAAA
                     RIGAILTPLPVQFRTHELRQTIELTEPKVFITTSSFGGHDHVAMIRGLQPEFPAVEHV
                     LALGEELPQGVSSLTAILTTETDTSAVDDYAGVHPVSANDVYTICWTSGTVAEPKGVP
                     RSHNLWIAIAWATVDAAQIREGDTLLNPFPLVNMSAIGGMLVPWLLTRGKLVMHQPLE
                     LPVFLAQLGGEQVTYTVAPPVLLNLLLLRPGMLENADLSHIRNIGSGSSPLSPWMVAQ
                     WQERHGIPILNFFGANEGTALASDPVDIPDPEERALYFPRLGVPGYAWSNRVGEMIET
                     KLLDPETRAVVTEPGEVGELAIRGATVFPGYWKRPQITADSFDDEGYFLTGDLFAIAG
                     DGEDRYRFVGRLKNLIIRGGMKIAPEEVEFLLADHPGIQEVAVLGVPDGRKLGEEVIT
                     ATVVPKEGAEVKLSDLTDFLRQKDIAAYKLPRRLVLVESLPHNAVGKVLKREIKRQLA
                     GATA"
     gene            complement(4064..>4267)
                     /locus_tag="VFZ86_00130"
     CDS             complement(4064..>4267)
                     /locus_tag="VFZ86_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020888034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5640726.1"
                     /translation="DAYRQTARITPLRTNVTQDDVGRTALWLCSDWARAVTGEIVYVD
                     AGYHILGATTRPDDADTPAPPAE"
BASE COUNT          667 a         1463 c         1454 g          683 t
ORIGIN      
        1 cgtggccttc cgggagaccg tcaacgtgcc gttggtgatc gtggatccgc gccaccctgg
       61 cgggggcagg acggacgcgg tgggttccca tctcgacctg gttcccacca tcctggcgta
      121 tgccggcctg ccggaggctg agcggcaaca gcgctaccca ttcctgaaag gacatgacct
      181 ttcagggatc gtggaggatc ccgcctcggc cggaccgcgt ggcgccagcg gggaacccgg
      241 caagggctgt ctgctgacct acgacatgat cgccaccgtc gacgcggaat ggttcagccg
      301 caacgcgacc agggtgttcg acgcagccgc agcgcaggcc ggcctggagt tccatcgcgg
      361 tatggactcg ttcaagggca tgctggatga gatcggcgtg ccggacctcg agaaacgaga
      421 gctgttccgc tgtgtcttcg atggtcgcta caagctggtc cggtacttcg ggttgggtgg
      481 ctacaacctg ccggcatcgg tcaagcagtt gctggccgac aacgacgccg ctctctacga
      541 tctgctactg gatcctgagg aaatggacaa tctggccgac ccggccaatc ccaagtacag
      601 cgaagaactc ttgtcggcca tgaaccagaa actcaatgcg ctgatcgatg aggagatcgg
      661 ggaagacaaa gccctgttca ccccatagat gagcgcctca gcctgtcgtg ggaacgtgcc
      721 caactccttg ggcgtccacg tcgcttgagc gccgcccgtg tcgccgggtc gatgtgggcc
      781 cgccggtagt ccggtgggaa gagaagtggt gcccccaacg gaattcgaat ccgtgccgcc
      841 ggcttgaaag gccggtgtcc taggccgcta gacgatgggg gcacgagggt gccgccgggg
      901 gagtatatcc tgcccagctc cgggccgccg tcggctgagg ctcaggcgct cttctcgcgc
      961 acgatgacct cgacctcgca gcccagcacc tggagcaggg acagcatctt gtcgaccgac
     1021 ttgcggtagt tggtcgtgtc gagcagccgg tagaattgcg ccggcgaggt accgagcctg
     1081 cggatgatct cgcgcttcga cagcgggctc gcctcgaggc ggtcgagggc ctcgcaggtg
     1141 aggttgtgga ggagcaggtc gcgcatgtac ccgggctcct cgttgtagtc gagcacctgg
     1201 tccatgagga cgcagccgtc tgttccggat gcaagctcat aggtgaaccc ttcacctccg
     1261 agctccgggt cgacaaacag cctctcgacg gggtcggagg tcgagggtgg ggggtctgcc
     1321 ttgctgtagg ggaagggcca tgcgccgcgc cgggtcgtga cctcgaaggt ccgcttgcgg
     1381 ttgttggccc tgacggcggt gatcttcaca ggagtccctc actctcgagt tctctgatca
     1441 gcgcgacgag cctcgcggtc gggacgccag ccatgggcct gttgcggtcg aggttccact
     1501 tgagcaccag gcgtccgtcg cgcagcacat gaacgtggcg tggggagtgg tcgcccaccc
     1561 aactgatgaa gacgtagcct ccacgccgca ctctcgtcac ggtaaatgtt acctcacagg
     1621 gtaacgcttg agaagacagg cgtctacgat gcccgcaggt cgatggtgtg gcgccgcggc
     1681 gaaggcaaca agacccggac atcgcgcggc ggtggacgat gtgacgacgg atcgggttgc
     1741 gatcgcgggc cggatgctag actccgcggc gtgagcacca ccgaccagac tcgccggcgt
     1801 ttcaccacgc cggtgcgtgt gcgctacgcg gacaccgacg cgacgggcac gctgcacccg
     1861 gccacctacc tgatgtactt cgaggtggcc cgggtggagg ccctgcgcgg gctcggggtg
     1921 ccggtcggcg agttcgcgac gcgcggcgtg gcgatgccgg tgatcgaggc gggcgtcaac
     1981 gtggtccgcc cggcccgcat cgacgacctg ctcgaggtcg acgtcctgct cgaccgcatc
     2041 ggccccgcgt ccttctcgtt cgagtacgag gtggcgcgcg acggcctcct gctcgccacc
     2101 ggcttcacgc gcatggccgt gatcgacccg gcggacgggc gcgccctgcg cctgcccgac
     2161 tgggtgcgcg acctgctgga gggcatcggc tcctgatgcc gctctcgcag cggctcgctg
     2221 cgtgaggttt cgtggtgccc gccgctgcgt acaactcccg catagattct gtccagacgc
     2281 tcgaatcccg accggcgtgc cgccggccga cccaggaggc gtgatgatcc tcgagtcccc
     2341 ggagcgcatc gccgagtaca ccgcccgcgg ctggtggggg gacaagaccc tcgccgacct
     2401 cttccttgag aacgtgcgcc gcacgcccga ggcggtcgcc gtcgtcgacc cgccgaaccg
     2461 ggcggcgctg accccggggg agccgcagcg cctgacctac ggcgagctca agaccgccgc
     2521 cgaccggctc gccaaggcgc tgctcgacgc cggcgtcggc cgcgacgacg tcgtgatggt
     2581 gcagctgccc aacatcgtcg agctggtcgt cgtgtacctc gccgccgccc gcatcggcgc
     2641 gatcctcacg ccgctacccg tgcagttccg gacgcacgag ttgcggcaga ccatcgagct
     2701 caccgagccg aaggtcttca tcaccaccag ttccttcggc gggcacgacc acgtcgcgat
     2761 gatccgcgga ctgcagccgg agttcccggc ggtggagcac gtgctggcgc tcggcgaaga
     2821 gctcccgcag ggggtctctt cgctcaccgc gatcttgacc accgagaccg acacgagtgc
     2881 ggtggacgac tacgccggcg tacaccccgt ctccgccaac gacgtgtaca ccatctgctg
     2941 gacctccggg acggtcgccg agcccaaggg ggtcccgcgc agccacaacc tctggatcgc
     3001 catcgcctgg gcgaccgtcg acgccgccca gatccgggag ggcgacaccc tgctcaaccc
     3061 cttcccgctc gtgaacatgt cggcgatcgg cggcatgctg gtgccctggc tgctgacccg
     3121 cggcaagctc gtgatgcacc agccgctcga gctgcccgtc ttcctggcgc agctgggcgg
     3181 cgagcaggtg acctacacgg tcgcgccgcc ggtcctcctc aacctcctcc tgctccgccc
     3241 cggcatgctc gagaacgccg acctcagtca catccgcaac atcggctcgg gctcgtcgcc
     3301 gctctcgccc tggatggtcg cgcagtggca ggagcgtcac ggcatcccca tcctcaactt
     3361 cttcggcgcc aacgagggca cggcgctcgc gagcgacccc gtcgacatcc ccgatccgga
     3421 ggagcgcgcc ctgtacttcc cgcgcctggg cgtccccggc tacgcgtggt ccaaccgcgt
     3481 cggcgagatg atcgagacga agctgctcga cccggagacg cgcgcggtcg tcaccgagcc
     3541 gggcgaggtc ggagagctgg ccatccgcgg cgccaccgtc ttcccggggt actggaagcg
     3601 gccgcagatc acggccgact cgttcgacga cgaggggtac ttcctcaccg gcgacctgtt
     3661 cgccatcgcc ggcgacggcg aggaccgcta ccgctttgtc gggaggctca agaacctgat
     3721 catccgcgga ggcatgaaga tcgctccgga ggaggtcgag ttcctgctgg ccgaccaccc
     3781 cgggatccag gaggtggcgg tgctcggcgt gcccgacggg cgcaagctgg gcgaggaggt
     3841 catcaccgcg acggtcgtgc ccaaggaggg cgccgaggtc aagctgagcg acctcaccga
     3901 cttcctccgg cagaaggaca tcgccgccta caagctcccc cggcggctgg tgctcgtgga
     3961 gagcctgccg cacaacgccg tcggcaaggt cctcaagcgc gagatcaagc ggcagttggc
     4021 gggcgcgacg gcctagcctg gtcgcccggc gcgacggtct ggcctactcg gccggcggcg
     4081 ccggcgtgtc ggcgtcatcc ggccgggtcg tcgcgccgag gatgtggtag ccggcgtcca
     4141 cgtagacgat ctcgccggtc acggcgcgcg cccagtcgga acacagccag agcgccgtgc
     4201 ggccgacgtc gtcctgggtc acgttcgtgc gcagcggcgt gatcctcgcc gtctgccggt
     4261 aggcgtc
//
LOCUS       DASUMX010000005        13660 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1152600, whole genome shotgun sequence.
ACCESSION   DASUMX010000005 DASUMX010000000
VERSION     DASUMX010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 13660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13660
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1152600"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..851)
                     /locus_tag="VFZ86_00135"
     CDS             complement(<1..851)
                     /locus_tag="VFZ86_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HEX5640727.1"
                     /translation="MSEQWTSVDGNEAAARVAYALSEVLAIYPITPSSPMGEAADSWA
                     AAGKPNLWGTVPDVVEMQSEAGAAGALHGSLQRGALSSSFTASQGLLLMIPNMFKIAG
                     ELTPAVIHVSARTVATHALSIFGDHSDVMHARTTGWAMLASASVQEAQDFALIAHAAT
                     LRARVPFLHFFDGFRTSHEIASIVLVSDDDIRALVSEEAILAFRGRALSPDAPLVRGT
                     SQNPDAFFQGREAGNSFYLDTPGIVAESMAAFERQTGRGYGLVDYVGAPDAERVVVLM
                     GSGIGAA"
     gene            complement(848..1357)
                     /locus_tag="VFZ86_00140"
     CDS             complement(848..1357)
                     /locus_tag="VFZ86_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5640728.1"
                     /translation="MRIHPARLTSPGAQVPALTEDTDLLLARLSHAGGARWARTYDGP
                     DPLDDGEESVATLGGDICVAGFTTSPATARDILTLKYGGGGQFRWARSHGGVDMSNDF
                     AAGLLAVSGGVYTAGKRGAGTGNDGMTLPTGGAIQPLLPAVMRLLPWNEHRFGNQRPA
                     RQSHPGGTS"
     gene            complement(1563..3158)
                     /locus_tag="VFZ86_00145"
     CDS             complement(1563..3158)
                     /locus_tag="VFZ86_00145"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5640729.1"
                     /translation="MNEPDGGRTYTRREVLQTSLLAGAGLALAPTLLACGGDSSDSAT
                     SASPSASAGPKKGGSLRVGTVGGSAKETPDGQIGGETVPNVALTHCMYDSLLTWSNDY
                     ALENLMADEVTVNGDASVWTVRIKDGVEFHNGKTVTADDIIWSYKRIIDPKDPKTGSA
                     NLAQLKPSGLRKIDDLTVEFTLTEPNAVFDEALGYYINCIMPQDWSAADPIGTGPFKL
                     TEFKPGEGFKFVRNENYFGQIPWVDELAIMEFADTTARVNALLDGTADAISDLPSAQI
                     KVVEAGGMKALDAKTGAWQPIYMNLKAKPFTDVRVRQAFKLIPDRQAMIEQAYAGLGS
                     LGNDMYAPFDPGYPTDIPQRTQDLEQAKSLLKQAGYSDLTVTLVTSDAVGSGVVAAAQ
                     VYAEMAKGAGVTVKVDKVDSAIIYGDRYLSWPFSQDFWYTHNYLTQVTSASLPTSPYN
                     ATHWADDEWSKIVNEAFRTGDVTKRNELIAQAEQIQFDNDGLIIWSFNDQVDGYSSKV
                     GGVVPDKGGVPLSGWRLNTYYFV"
     gene            3325..4212
                     /locus_tag="VFZ86_00150"
     CDS             3325..4212
                     /locus_tag="VFZ86_00150"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEX5640730.1"
                     /translation="MTMEKEGAVVAESKYIYAQMTWPEIGRVAEEDRVVVIPIATMED
                     HGPHLPIDTDVKLIESMCAAACARIPDKVVLLPVVTHGYSPHHGDFPGSINIRWNVFV
                     EHLTDITNSLVGHGFRRFVLANGHGSNMPLVNMAARLTIVQHPGTIACDYMYLLTPRG
                     VDAIKSVRESAFPGGMSHACELETSMYLHLDPALVQMELAEKDISFPESDYFYFDWFD
                     GPGSMMEYWSTLSRTGTMGDPTLATAEKGKVFFEAAVDELLMVIGEMKKREIRQRVDH
                     HGPRSGRPGAGASEGAARA"
     gene            4209..5201
                     /locus_tag="VFZ86_00155"
     CDS             4209..5201
                     /locus_tag="VFZ86_00155"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX5640731.1"
                     /translation="MSIELDELDRHIVEALRRDGRRSSSSVARELGVSKQTVAKRLDR
                     LLKHDAIRITARVDPVAFGFPICCGIGVRVRPGAMERVREQLAAMDHVAWVGSSTGSY
                     DVLADAFLPDTEAVFEFLHEHLASMPEIVETREWLVLRSAKYVYLWDHQEQGAGAEAL
                     RGWTTKPGSTLEPARVDDLDRAIIGLLRQDGRRPFTDIARRVRVTEATVASRVDRLAR
                     TGAMQIIAHVNWPAIGFPVDVSLSIKAARGRVEEVGTRLRELPYVSFAGYTTGDYDII
                     AEAFLPDDASLLDFLNDHVGGIPGVESTDTCHILRVAKVNYMWEGERISHTARA"
     gene            complement(5335..5994)
                     /locus_tag="VFZ86_00160"
     CDS             complement(5335..5994)
                     /locus_tag="VFZ86_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5640732.1"
                     /translation="MRHRIAAPIVVVALVLLLALPSIASAWTATGFSTVTVKEDSFYN
                     YDCETTTASFDTVDWPVMFVFYGNATVAKVDAALSSRLPIWGIDEYMNLNDGAGWQWV
                     ANTGVKSLSLSRAVHLRLYAPGGQNFTNKTWGKYVIGTCHVDINELSSSPTSGYSEDA
                     ATEIAAMCQSIWGKRNVAANSYAMGNAEPLRTEERTKSNGDIEHHVWQSDGYATLVKV
                     R"
     gene            complement(6020..6829)
                     /locus_tag="VFZ86_00165"
     CDS             complement(6020..6829)
                     /locus_tag="VFZ86_00165"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016872561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HEX5640733.1"
                     /translation="MKVESGRRLRRSICIALLLVLAVVLISASAALAITYGVPDGDGH
                     PGVGALVAELDGQKVAVCSGALISPNVFLTAAHCTAALESWGVTDVWVTFDPVFDAED
                     GTFVPGEMRTNPGYSHRRSDTGDVAVILLDTPQDARPIAQLPTAGQFDLMAAQNGLRG
                     QTFRAVGYGAQELVFTKGKPYFGPNGTRMTAVSTFDALTNAWLHLSANPAKGDGGTGY
                     GDSGGPNFLGESDVIAGLTVTGDAVCRATNVIYRLDTPSARGFLAQYVTLP"
     gene            7036..7491
                     /locus_tag="VFZ86_00170"
     CDS             7036..7491
                     /locus_tag="VFZ86_00170"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX5640734.1"
                     /translation="MDINRTAPVTAKTQMQIAADRQTVWDALTDFETWPEWKRDVRSM
                     SLRGPVAPGSTFEWKAGPGTIRSTLEEVDAPGRIAWTGSTFGIKAVDVFRLEARDGGT
                     LVTEEESWEGLLARLFRSRMRRTLQSGIDQGIEALKAEAERRARQSAAA"
     gene            7729..8439
                     /locus_tag="VFZ86_00175"
     CDS             7729..8439
                     /locus_tag="VFZ86_00175"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEX5640735.1"
                     /translation="MTAALLAALTLALVALAGCGAAGTTTAAGATTTNTVTASGAGTA
                     QAVPDTAEMSFGVTTTSPNAKSALDDASRSAEQIASAVKKQGVADEDIQTRDVSVYPQ
                     TVDDNGKQVITGYQASLSVAVKVRDISKLGDVISAANAAGANNISGPAFSIGDPAPAR
                     AEAIDEAVADARKSAEAMAKAAGKSVGEVLSMSSSDVGLVPGPMYSQDMAAAARDVPI
                     EPGQLDISASVVVVFELK"
     gene            8622..12794
                     /locus_tag="VFZ86_00180"
     CDS             8622..12794
                     /locus_tag="VFZ86_00180"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEX5640736.1"
                     /translation="MALVTVLAVVDPALAANGTATGRVTEIGTGLPLADVRAYFNGLD
                     PENGGVAVNALTTADGTYSLSLPAGGYRVMFVRDFMGWWEVYYNGHDSVDLADRVTVV
                     AGKTTVGVNGQFAHIAAGWLRGTIRDAGTGTPLSNAVVAIHTWDGQWYSPDYYAYADS
                     TGSFAKQLPAGTYRLGFSYPGYTERYYVDHPVFADADPVVVGDGGTTTVDATLAVIPG
                     WVEGVTRDFNGNIVAGIVVDVLDASTGALVATGMSDQDGAYSLGPITGAPGLKLVVRF
                     TDPGSTFPVQYGGGVTQQAAATRYDAEPGYFRALVSPRLFTGTASIQGTLRDVDGRPI
                     SGVWVSARRKIDSATIVAVKTGADGRYLVAPLPVAGTTTYTLDFVSSDDGHFHEYWQN
                     ATSSDTATPIELSAGTALTIDEVLETMCSIKGTVSDHRGPLHRISVTLYDDQGEAVEL
                     AQTYSTGEYAFEGLRVGAYRVGFNDPTGRHVAEYHADAADLDAATPVVLSAAAPDATV
                     PATLLPTTISGTVRGVVVLQHIMVTLYDADGVALRTASTDGSGIYDFGPVAHGDYRIG
                     FEDPDGEWKPEYYDDAASLGAATVVRLTAATPYAVADAQLTANMSIRGSVGPDTHSAW
                     LPPWPDDIEVTLYRLDGSVVDTLVTGGGFQFGALVPGDYRLLFVDPSGQYVNEYYGDA
                     PGCALAAATIHVGDADPHVYAEERLQPAAGPPGAKPDGSAEVANEIIGPVNNGGLGSA
                     LDMAGDTLVVGAKWQWLGDSGHNPGVAYVYDRRGGAMIRTSALSSPDPTDYGCFGSDV
                     AIEGDRIAVAEFRDDGRGLDPGVVHVFERDAIGWELAATLRPGGSDGLLHRGFGASLS
                     MSGDTIIVGAPYAKLNGDTEAGVVYVFTRSGGVWKQQARLTAASPSDDDFFGRAVAVH
                     GDRLVVGADMDDTVAGVNAGSAFVFTRSGATWTRRATLRPSDADIGIAFGGKVAISAD
                     TIMVAAAGQLYDQGAVYVYEPSGDTWIERARLTAGDGQPNDSFGRSLALDGDTAVVGS
                     TAEGSDDASGQTTNGAAYRFTRIGGSWYQEARLAADKGALSRGVGTAVAVCGRVIAVS
                     DTAFRKDPELAHSGAVRLFEPHRTAVDSALVVAAEDGVLKNDQSPSSEPLQARLVDAD
                     GAVLGDRAATAHGAVDLSFDGAFSYVPSAGYTGADGFFYQAYTSEWQSEATPVTVTVN
                     DLTAPAITAADVARGWVNTPVTVTLSTGAATDVAAAEYRRRGPNLWQRYLEPFQVSRQ
                     GASTYDIRARDVYGNSATRAFMVRVDTKRPTPQAPTAATVTRGRTATLKYKIVDARPG
                     SPTATVTIRIRNARNAEKWRVILKNKPVNKLLGYAFRCSLPRGTYRFLVTATDAAGNR
                     ETRSASNKLVVR"
     gene            complement(13309..>13660)
                     /locus_tag="VFZ86_00185"
     CDS             complement(13309..>13660)
                     /locus_tag="VFZ86_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5640737.1"
                     /translation="RELGLTVTGPDGQTVPVKSYLLGTKVASIAVHDHYDEYAAQPRF
                     AVATFRADQTGGYEIATTSAAENGAEIAVGENLVRPVLFGLFGALAVVLAGVATGVVL
                     IIVTAVRRTAPRAA"
BASE COUNT         2185 a         4628 c         4715 g         2132 t
ORIGIN      
        1 tccgccgcgc cgatcccgga gcccatgagg acgacgacgc gctcggcatc gggcgcgccc
       61 acgtagtcca ccagcccgta gccgcggccg gtctgccgct cgaacgccgc catggactcg
      121 gcgacgatgc cgggcgtgtc caggtagaac gagttgccgg cctcgcgtcc ctggaagaac
      181 gcgtcgggat tctgggacgt cccgcgcacg aggggcgcgt ccgggctgag ggcgcggccg
      241 cgaaacgcga ggatcgcctc ctcggacacc agcgcccgga tgtcgtcgtc gctgacgagg
      301 acgatgctcg cgatctcgtg cgaagtgcga aagccgtcga agaagtgcag gaacgggacg
      361 cgcgcgcgca gcgtggcggc gtgggcgatg agggcgaagt cctgggcctc ctgcaccgac
      421 gcggacgcga gcatcgccca acccgtcgtg cgcgcgtgca tgacgtcgct gtgatccccg
      481 aagatcgaga gcgcgtgcgt ggccacggtg cgcgccgaga cgtggatcac cgccggcgtc
      541 agttcgccgg cgatcttgaa catgttgggg atcatcagca gcagcccctg cgaggccgtg
      601 aagctgcttg acagcgcgcc tctctgcagc gagccgtgca gcgcgccggc ggcgcccgcc
      661 tcggactgca tctcgaccac gtccgggacg gtgccccaga ggttcggctt cccggccgcg
      721 gcccaggagt ccgcggcctc gcccatgggc gaggacggcg tgatcgggta gatcgccagc
      781 acttcactga gagcgtaggc gacgcgcgcg gcggcctcgt tcccgtcgac agaggtccac
      841 tgctcgctca cgatgtgcct ccaggatgag actgccgggc aggacgctgg ttcccgaagc
      901 gatgctcatt ccatggtagc aggcgcatca cggcgggaag caagggttgg atcgcgcctc
      961 ccgtcggcaa cgtcatcccg tcgttgcccg tcccggcgcc gcgcttgcct gccgtgtaca
     1021 cgcccccgct cacggccaga agccctgcag cgaagtcgtt ggacatgtcg accccgccgt
     1081 ggctccgcgc ccagcggaac tgaccacccc cgccgtactt gagcgtgagg atgtcccgcg
     1141 cagtggcggg actggtggtg aaaccggcga cgcagatgtc gccgcccagg gtggccacgc
     1201 tctcctcgcc gtcgtccaga ggatcgggtc cgtcgtaggt ccgtgcccag cgcgcgccgc
     1261 ccgcgtgaga caggcgagcc agcagcaggt cggtgtcctc ggtcagtgcg gggacctgcg
     1321 caccgggcga ggtcagccgg gcagggtgta tgcgcaaccg gtgtcgcaca ggcggccgta
     1381 cgcccgcaag tacaacggta cgcctgaagg cacctccggg cgtgcgagtc tctgcgacgc
     1441 accagtctcg ccggcggacg ccgggtctcg aagcggggga ccggatggct ccgactgaag
     1501 aggggcgagg cgcccaggca ccccgccccc cgtttcgccc tcttagtctt tccgccggag
     1561 cgtcacacga agtagtacgt gttcagccgc cagcccgaaa gcggaacgcc gcccttgtcg
     1621 gggaccacgc cgccgacctt gctggagtac ccgtcgacct ggtcgttgaa cgaccagatg
     1681 atgaggccgt cgttgtcgaa ctggatctgc tctgcctgag cgatcagctc gttcctcttc
     1741 gtcacgtcac ctgtgcggaa cgcctcattg acgatcttgg accattcgtc gtcggcccag
     1801 tgggtagcgt tgtagggcga ggtgggcaga gaggccgacg tcacctgggt caggtagttg
     1861 tgcgtgtacc agaagtcctg cgagaacggc cagctgagat accggtcgcc gtagatgatc
     1921 gccgagtcga ccttgtcgac cttgactgtg acgcccgctc ccttggccat ctcggcgtag
     1981 acctgcgctg cggccaccac gccgctccct accgcgtctg acgtgacgag cgtcacggtg
     2041 agatcgctgt accccgcctg cttgagcagc gacttggcct gctccaggtc ctgcgtccgc
     2101 tgggggatgt cggtggggta gcccgggtcg aacggcgcgt acatgtcgtt gccgagcgac
     2161 ccgagacccg cgtaggcctg ctcgatcatg gcctgtctgt cggggatgag cttgaaggcc
     2221 tgccggacgc gtacgtcggt gaagggcttg gccttcaggt tcatgtagat cggctgccac
     2281 gcgcctgtct tggcgtccag cgccttcatg ccgccggcct cgacgacctt gatctgcgcg
     2341 ctcggcaggt cggagatcgc gtccgcggta ccgtcgagaa gcgcgttgac acgggcggtg
     2401 gtgtcggcga actccatgat ggcgagctcg tcgacccagg ggatctgacc gaagtagttc
     2461 tcgttgcgga cgaacttgaa cccctcgccg ggcttgaact cggtgagctt gaaaggcccg
     2521 gtgccgatcg gatccgcggc gctccagtcc tggggcatga tgcagttgat gtagtagccg
     2581 agagcctcgt cgaacaccgc gttgggttcg gtcagcgtga actcgaccgt caggtcgtcg
     2641 atcttgcgga ggccgctcgg cttgagctgc gcgaggttcg cggagcccgt cttggggtcc
     2701 ttgggatcga tgatgcgctt gtagctccag atgatgtcgt cggcggtgac cgtcttaccg
     2761 ttgtggaact cgacgccgtc cttgatgcgc acggtccaca ccgacgcgtc gccgttgacg
     2821 gtcacttcgt cggccatgag gttctcgagc gcgtagtcgt tgctccacgt gagcagcgag
     2881 tcgtacatgc agtgcgtgag agccacgttc gggacggtct cgccgccgat ctgcccgtcg
     2941 ggggtctctt tggcggaccc gccgacggtg ccgacgcgga gactgccccc cttcttcggc
     3001 ccggcgctgg ccgatgggct cgccgaagtg gcgctgtcgc tcgagtcgcc gccgcacgcc
     3061 aggagcgtgg gggccaaggc gagccccgcg ccggcgagga ggctcgtctg gagcacctcc
     3121 cgccgcgtgt acgtcctgcc gccatccggt tcattcacgg cttccccctt ggtctcgccg
     3181 atggacgctg ccccctggca ccagcagtcg gcgacacctt agcacccatt ccggtgttca
     3241 atcaaggaat agcgtgtatt ctggatggca gaaccggacc tttggtggac atcatgacac
     3301 caaaccggga tgaggagtct ggtgatgacc atggagaagg agggtgctgt ggtggccgag
     3361 agcaagtaca tctacgcgca gatgacgtgg cccgagatcg gcagggtggc cgaggaggac
     3421 cgggtggtcg tgatcccgat cgccacgatg gaggaccacg gcccccacct gcccatcgac
     3481 acggacgtga agctgatcga gtccatgtgc gccgcggcgt gcgcccggat ccccgacaag
     3541 gtggtgctgc tgcccgtggt cacgcacggc tactcgcccc accacggcga cttccccggg
     3601 tccatcaaca tccgctggaa cgtcttcgtc gagcacctga cggacatcac gaacagcctg
     3661 gtcggacacg ggttccgccg cttcgtcctc gccaacgggc acgggtccaa catgccgctg
     3721 gtgaacatgg cggccaggct gaccatcgtc cagcaccccg gcaccatcgc ctgcgattac
     3781 atgtacctgc tgaccccccg aggcgtcgac gccatcaaga gcgtgcgcga gagcgccttc
     3841 cccggcggca tgtcgcacgc gtgcgagctc gagacatcga tgtacctgca cctcgaccca
     3901 gccctcgtgc agatggagct cgcggagaag gacatcagct tcccggagtc cgactacttc
     3961 tacttcgact ggttcgacgg ccccggctcc atgatggagt actggtccac actctcgcgc
     4021 acgggtacga tgggcgaccc gactctcgcg acggcggaga agggcaaggt gttcttcgag
     4081 gcggcggtgg acgaactgct catggtgatc ggcgagatga agaagcggga gatccgccag
     4141 cgggtggacc atcacggccc tcgctccggc cggccaggcg ccggtgcgtc cgaaggcgcc
     4201 gcgagggcat gagcatcgag ctcgacgagc tggatcgtca catcgtggag gcgctgcggc
     4261 gcgacggacg ccgcagcagt tcgagcgtgg cgcgcgagct gggcgtctcc aagcagacgg
     4321 tggcgaagcg cctcgatcgt ctgctcaagc acgacgccat ccgcatcacc gcccgcgtcg
     4381 acccggtcgc cttcggcttc ccgatctgtt gcggcatcgg cgtccgcgtc cggccgggcg
     4441 cgatggagcg cgtgagggag cagctcgcgg ctatggacca tgtcgcctgg gtggggtcgt
     4501 cgacgggcag ctacgacgtg ctcgcagacg cattcctgcc ggacaccgag gccgtattcg
     4561 agttcctgca cgagcacctt gcgagcatgc ccgagatcgt ggagacgcgg gagtggctcg
     4621 tgctccgcag cgccaagtac gtgtacctgt gggaccacca ggagcagggg gccggagctg
     4681 aggcgctgcg cggctggacg acgaagcccg gatccaccct cgagccggcg cgggtcgacg
     4741 acctggaccg cgccatcatc ggcctgctca ggcaggacgg gcggcggccg ttcaccgaca
     4801 tcgctcggcg ggtccgcgtc accgaggcga ccgtcgcgag ccgcgtcgac cgtctggctc
     4861 gcaccggcgc catgcagatc atcgcgcacg tcaactggcc ggccatcggt ttcccggtgg
     4921 acgtgagtct cagcatcaag gcagccaggg ggcgggtgga agaggtcggc acccgcctgc
     4981 gggagctgcc gtacgtctcc ttcgcgggtt acacgaccgg ggactacgac atcatcgccg
     5041 aggccttcct ccccgacgac gccagcctgc tcgacttcct gaacgaccac gtgggcggca
     5101 tccccggcgt cgagtcgacc gacacgtgcc acatcctccg cgtggccaag gtcaattaca
     5161 tgtgggaagg ggagcggatc agtcacacgg cacgggcctg aacgaaccga gaccaccggc
     5221 gcgtggcggt ctcggtgttt ccctgcgctc gaagcaggtg acgatgagcg acgtggacga
     5281 tcgactggcg ttgttgcagg cgtgccgcgg acggcggtca gtgtccgcgg cctgctaccg
     5341 caccttcacc agggtggcgt acccatccga ctgccacacg tgatgctcga tgtcgccgtt
     5401 gctcttcgtc cgctcctcgg tgcgcaaggg ctcggcattg cccatcgcgt agctgttggc
     5461 cgccacgttg cgcttgcccc agatgctctg gcacatcgcc gcgatctcgg tggccgcgtc
     5521 ctcgctgtag ccggacgtgg gtgacgaact gagctcgttg atgtccacgt gacaggtccc
     5581 gatgacgtac ttgccccagg tcttgttggt gaagttctgg ccaccgggtg cgtagagcct
     5641 cagatgcacg gcgcgcgaca gactcagcga cttcacaccc gtgttcgcca cccactgcca
     5701 gccggcgccg tcgttgaggt tcatgtactc gtcgatgccc cagatcggca ggcgcgacga
     5761 cagagcggca tcgaccttcg cgacggtggc gttgccgtag aagacgaaca tcaccggcca
     5821 gtcgacggtg tcgaagctgg ccgtggtggt ctcgcagtcg tagttgtaga agctgtcctc
     5881 cttcaccgtg acggtgctga acccggtggc cgtccaggcg ctcgcgatcg aaggaagggc
     5941 cagcaacagg accagggcga cgacgacgat aggcgcggct atccggtgtc tcatggttgc
     6001 ctcctgactc gcggggtcgc tacggcagag tgacgtactg cgcgaggaat cccctcgcgg
     6061 agggcgtgtc cagcctgtag atgacgttcg tggctcggca cactgcgtcg ccggtcactg
     6121 tgagtcctgc gatcacgtct gactccccga ggaagttggg gccgccggag tcgccataac
     6181 cggtgccgcc atcgcccttc gccgggttgg ccgaaaggtg gagccaggcg ttggtgagcg
     6241 cgtcgaacgt ggagacggcc gtcatacgcg ttccattcgg gccgaagtag ggcttgccct
     6301 tcgtgaagac gagctcctgg gcgccgtagc cgaccgcccg gaaggtctga cctcgcagcc
     6361 cgttctgcgc cgccatgagg tcgaactggc cggcggtcgg cagttgcgcg atcggacggg
     6421 cgtcctgggg cgtgtcgagg aggatcactg cgacgtcccc ggtgtccgac cgcctgtggc
     6481 tgtatccggg gttggtgcgc atctctccgg ggacgaatgt gccgtcctcc gcgtcgaaca
     6541 ccggatcgaa ggtcacccag acgtcggtga cgccccagga ctccagggcc gccgtgcagt
     6601 gcgccgccgt aaggaagacg ttcggcgaga tgagcgcgcc cgaacagacg gccaccttct
     6661 ggccgtcgag ctctgcgacg agcgcgccga cccccggatg cccgtcgccg tcaggcaccc
     6721 cgtaggtgat cgcgagcgcc gctgacgcgg agatcagtac gaccgcgagg acgagcagca
     6781 acgcgataca gatggaccgg cgcagacgcc ggccgctctc cactttcatg ggtcctccct
     6841 ctctcgctcg gccttcggta gatcagctcc gtgaagggaa ctgtccaagc accatagtgg
     6901 tcacctgtcg ctgccggcat cacccgatga gggtgaagtc cgagcgccgg cctcgatgac
     6961 gccccatccc gttgcgtccg tctcgtcgcg ggtacagttc gcagcattgg caagcacgat
     7021 tcgaggcaga ggccgatgga catcaatcgc acagcaccag tcacggccaa gacccagatg
     7081 cagatcgcgg ccgatcggca gaccgtgtgg gacgcgctca ccgacttcga gacttggcca
     7141 gaatggaaac gcgacgtccg ttcgatgtcg ttgcgggggc ctgtcgcccc gggttcgacc
     7201 ttcgagtgga aggccggacc agggacgatc aggtccacgc tcgaggaggt ggacgctcca
     7261 ggcaggatcg cgtggacggg atcgaccttc ggcatcaagg ccgtcgacgt cttccgtctc
     7321 gaggcccgcg acggcggcac gctggtcaca gaggaggagt cgtgggaagg cctgctcgcc
     7381 cggctgttcc gctcgcggat gcgtcggacc ctgcagtcgg ggatcgacca gggcatcgaa
     7441 gcgctgaagg ccgaggccga gcggcgcgcg aggcagagtg ctgccgcctg actcgccggc
     7501 gccttggttg atcgcgcgcc tgcagccgtt ctgcagccct gatcccgtga ctcgatggat
     7561 caggcgggtc cgccgtgctc gcgcggcgtc acgtgcccct gagcgcgcat ggccgcgaac
     7621 ctctgcctgc acatggccgt cctatgagca gggggtacgg aaggcgcagc cgctcgcggc
     7681 gcgcgcacga tctcaagggg gaacacgatg gcactcggga acaggtatgt gaccgctgca
     7741 ctgctcgcgg ctctcacgct ggcgctcgtc gcgctcgccg gctgcggggc ggccggcacc
     7801 actaccgcgg caggcgccac caccaccaac accgtgaccg cctccggcgc cggcaccgcc
     7861 caggcggtcc cggacacggc cgagatgtcc ttcggcgtca ccacgacgtc gcccaacgcc
     7921 aagtcggctc tcgacgacgc ctccaggagc gccgagcaga tcgcctcggc ggtgaagaag
     7981 caaggcgtcg ccgacgagga catccagaca cgcgacgtca gcgtctaccc gcagacggtc
     8041 gacgacaacg gcaagcaggt catcaccggc taccaggcat cgctgagcgt cgccgtcaag
     8101 gtgcgtgaca tctccaagct cggcgatgtc atctcggctg caaatgcagc aggcgccaac
     8161 aacatcagcg ggcccgcgtt ctccatcggc gatccggcgc ccgcccgcgc ggaggcgatc
     8221 gacgaggccg tggccgacgc gcggaagagc gccgaggcga tggcgaaggc cgccggaaag
     8281 agcgtcggag aggtgctgag catgagctcg agcgatgtgg gcctggtccc cggcccgatg
     8341 tacagccagg acatggccgc ggcggccagg gacgtgccca tcgagccggg tcagctcgac
     8401 atcagcgcga gcgtggtggt ggtcttcgag ctcaagtagc gcccgcagga cggaagcggg
     8461 ccgttgtctc ggcggatgac tgtttgtcgc agcaaggtat ccggcctgcg ttatgatgtt
     8521 cgtacacgcc gcgccgaacg ggggggaaca tgcgcgctgt acgcattccg gatcgctccg
     8581 gtcacgcacg tctcggtctt ccgcttgtcc tgctcgttct cgtggcgctc gtcacggtgc
     8641 tggccgtcgt cgatccggcg ctcgcggcga acggtacggc cacaggacgg gtcacggaga
     8701 tcggcaccgg tcttcccctg gccgatgtgc gcgcgtactt caacggcctg gatcccgaga
     8761 acggcggcgt cgccgtcaac gcgctgacca ccgccgacgg cacctacagc ctctcgctgc
     8821 cggccggcgg ctatcgcgtc atgttcgtga gggacttcat gggctggtgg gaggtgtact
     8881 acaacggcca tgattccgtc gacctggcgg atcgcgtgac ggtcgtcgcc ggcaagacca
     8941 cggtgggcgt gaacggccag ttcgcacaca tcgcagcggg ttggctgcgc ggcaccatcc
     9001 gtgatgccgg caccgggacg ccgctgtcga acgcggtcgt ggcgatccac acgtgggacg
     9061 gccagtggta cagcccggac tactacgcct acgccgactc gaccggctcc ttcgccaagc
     9121 aactgcccgc cgggacgtac cgtctcgggt tctcgtaccc gggttacacg gagaggtact
     9181 acgtcgacca tccggtgttc gccgacgccg acccggtggt ggtcggcgat ggtgggacga
     9241 ccaccgtgga cgcgacgctg gccgtgattc cgggctgggt cgagggagtc acgcgcgact
     9301 tcaacggcaa catcgtggcg ggcatcgtcg tcgacgtcct cgatgcatcg actggcgcgc
     9361 tggtcgcgac cggcatgagc gatcaggacg gggcgtacag cctggggccg atcaccgggg
     9421 ccccggggct caagctcgtc gtccgcttca cggaccccgg ctcgaccttc cccgtgcagt
     9481 acggcggcgg cgtcacgcag caggccgccg ccacccgcta cgacgcggaa ccagggtact
     9541 tccgggcgct cgtcagtccg aggctcttca ccgggacggc ctcgatccag ggcacgctgc
     9601 gggatgtcga cggaaggccc atctccgggg tctgggtcag cgcccggcgc aagatcgatt
     9661 ctgccaccat cgtggcggtg aagaccggcg ccgacgggcg ctacctcgtg gcccccttgc
     9721 cggtagcagg aacgaccacg tacaccctcg acttcgtctc ctccgacgac gggcacttcc
     9781 acgagtactg gcagaacgcg acgagctccg acaccgccac accgatcgag ctgagcgcgg
     9841 gaaccgcgct gacgatcgat gaagtgctcg agacgatgtg ttcgatcaag gggacggtct
     9901 ccgaccacag gggtccgctg caccgcatct ccgtcacgct ctacgacgat cagggagagg
     9961 ccgtcgagct ggcgcagacg tactcgacgg gcgagtacgc gttcgagggc ctgcgggtcg
    10021 gtgcgtaccg ggtcggcttc aacgatccga ccgggcggca tgtggccgaa taccacgccg
    10081 atgccgccga cctggatgcc gcgacgcccg tcgtcctcag cgcggcggcg ccggacgcga
    10141 ccgtccctgc gacgctgctg ccgacgacga tcagcggcac cgtccgtggg gtcgtcgtgc
    10201 tccagcacat catggtcacc ttgtacgacg ccgacggtgt cgctctcagg accgcgtcga
    10261 cggacggcag cggcatctac gacttcggcc ccgtcgcgca cggcgactac cgcatcggct
    10321 tcgaggaccc ggatggagag tggaagccgg agtactacga cgatgccgcg tcgctgggcg
    10381 ccgcgaccgt cgtgcgtctc accgcggcca cgccctacgc ggtcgccgac gcgcagctga
    10441 cggcgaacat gagcatccgc ggctccgtcg gacccgacac acactccgct tggctgccgc
    10501 cgtggccgga cgacatcgag gtgacgctgt accgcctgga cggcagcgtg gtggacaccc
    10561 tcgtgactgg aggcggcttc cagttcggcg cgctcgtacc cggcgactac cgcctcctgt
    10621 tcgtcgaccc aagcgggcag tacgtgaacg agtactacgg cgacgctccg ggctgcgcgc
    10681 tcgccgcggc gacgatccac gtcggcgacg cggatcctca tgtctatgcc gaagaacgac
    10741 tccagccggc cgccggaccg cccggagcga agcccgacgg ctccgccgag gtcgccaacg
    10801 agatcatcgg gccggtcaac aacggcgggt tgggcagtgc cctggacatg gccggagaca
    10861 ccctcgtcgt cggcgccaag tggcagtggc tgggcgattc cgggcacaac ccgggcgtgg
    10921 cgtacgtcta tgaccgacgc ggcggcgcga tgatcaggac gtcggccctc tcttctccgg
    10981 acccgacgga ctacggctgc ttcggcagcg atgtggcgat cgagggcgac aggatcgcgg
    11041 tcgccgagtt ccgtgacgac ggccgggggc tcgacccggg cgtggtgcac gtgttcgagc
    11101 gagacgcgat cggctgggag cttgcggcga cgctccgtcc gggcggcagc gatgggctgc
    11161 tgcaccgcgg atttggcgcg tccctgtcca tgagcggcga cacgatcatc gtcggggcgc
    11221 cgtacgcgaa gctgaacggc gacaccgagg caggtgtcgt ctacgtcttc acgcggagcg
    11281 gcggtgtgtg gaagcagcaa gccaggctca ccgccgcgtc acccagcgac gacgacttct
    11341 tcggccgcgc cgtcgccgtt cacggcgacc ggctggtcgt cggcgccgac atggacgaca
    11401 ccgttgccgg cgtcaacgcc ggatccgcct tcgtcttcac ccgatccggc gccacgtgga
    11461 cgcggcgcgc gacgctgcgg ccgagcgacg ccgacatcgg gatagcgttc ggcggcaagg
    11521 tcgcgatctc ggccgacacc atcatggtcg cggccgccgg gcaactgtac gaccagggcg
    11581 ccgtgtacgt gtacgagccc tccggcgaca catggatcga gcgggcgcgc ctcaccgccg
    11641 gcgacggcca gccgaacgac agcttcggcc ggtcgctggc gctcgacggc gacaccgccg
    11701 tggtggggtc gaccgccgag gggtccgatg acgcgtccgg ccagacgacc aacggcgcgg
    11761 cgtacaggtt cacgcgcatc ggcgggagct ggtatcagga ggcccgcctg gccgccgaca
    11821 agggagcgct atcacgcggc gtcggaaccg ccgtggcggt ctgcgggcgg gtcatcgcgg
    11881 tcagcgacac cgccttccgc aaggatccgg agctcgcgca cagcggcgcg gtgcgcctgt
    11941 tcgagccgca ccggaccgcc gtggactcgg cgctcgtcgt cgccgcggag gacggggtgc
    12001 tgaagaacga ccagagcccg tcctccgaac ctctgcaggc gcggctggtg gacgcggacg
    12061 gcgccgtgct cggagatcgt gcggcgaccg cccacggggc ggtggacctg tcgttcgacg
    12121 gcgccttctc ctacgtgccg tcggccggct acaccggcgc cgacggcttc ttctatcagg
    12181 cgtacacgtc cgagtggcag tcggaagcca cgccggtcac ggtcaccgtc aacgatctca
    12241 ccgctccggc catcacggcg gcggacgtgg ccaggggctg ggtcaacacc cccgtcaccg
    12301 tcacgttgtc gaccggcgcc gccacggacg tcgccgctgc cgagtaccgg cgtcgcggac
    12361 ccaacctctg gcagcgctac ctggagccgt tccaggtctc tcgccagggc gccagcacct
    12421 acgacatccg cgcgcgggac gtgtacggga acagcgccac ccgcgctttc atggtccgcg
    12481 tcgacacgaa gcgtcccacc cctcaggcgc cgacagcggc gacggtgacg cgcggccgca
    12541 ccgccacgct caagtacaag atcgtggatg ccaggcccgg aagccccacc gccacggtga
    12601 cgatcaggat ccgcaacgca cgcaatgcgg agaagtggcg ggtcatcctg aagaacaagc
    12661 ccgtgaacaa gctgctcggc tacgcgttcc gctgctcgct gccccgcggg acctaccgct
    12721 tcctcgtgac ggccaccgac gctgccggca atcgcgagac caggtcggcg agcaacaagc
    12781 tggtggtgag gtaggggcgg gaccggcggg gaccctggtc gacgccgacg acagcttcac
    12841 ggtggcccgg tcggcgtgac acaggccatc gacctgcgcg aggtcgtgtg gaacgacgcg
    12901 cgcagaccca cgcgaccccg cggcaactgg gacatctacg cgtgcgcctt gagctcggcc
    12961 tcttacttga agcctcgcca cgcggccggg acgcgggccg tcgcgatgct ctgctatcag
    13021 ttgcagggca acgggacctg gaagtacgtc aagaccgtca ggctgaaggt gaaggactac
    13081 cgtacgtaca gcaggtacag cgggatcatc gccctgaagg ctggccgatg gaaggtggaa
    13141 gcagtgcaca acgatgccga cactggagca cggagaccgc gttcggcagg acgatcggga
    13201 cctcgatgta gcgcggcggc tcggctgccg gcatcctcgg cgaagcgacg gggccggctc
    13261 gctggagcga accggccccg tcgcgacttc tctgtgaccc gcccgggttc aggcggcacg
    13321 cggcgccgtg cggcgcaccg ccgtcacgat gatcaggacc acgccggtcg cgacgcccgc
    13381 caggacgacg gccagcgcac cgaacagccc gaacagcacc ggccgcacca ggttctcgcc
    13441 gactgcgatc tccgcaccgt tctcggcggc cgaggtcgtc gcgatctcgt acccgccggt
    13501 ctggtcggcc cggaaggtgg ccaccgcgaa ccggggttgc gccgcgtact cgtcgtagtg
    13561 gtcgtggacc gcgatcgacg cgaccttcgt gccgagcagg tagctcttga ccgggaccgt
    13621 ctggccgtca gggccggtca ccgtgagacc gagctcgcgc
//
LOCUS       DASUMX010000006         3410 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_721408, whole genome shotgun sequence.
ACCESSION   DASUMX010000006 DASUMX010000000
VERSION     DASUMX010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3410
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_721408"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..816)
                     /locus_tag="VFZ86_00190"
     CDS             complement(<1..816)
                     /locus_tag="VFZ86_00190"
                     /inference="COORDINATES: protein motif:HMM:NF016158.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cryptochrome/photolyase family protein"
                     /protein_id="HEX5640738.1"
                     /translation="MTRAALIYPHQLFAEHPALAGVSRAVLVEEPLLFTHYRFHRQKL
                     VLHRATMKRFAAGLRRRQVKVHYVEAGRLADTAAIAEVLHRLRITSVRYVDPCDDWLA
                     ARLGDALERRGIEAFVVDDPHSLTPPSLVRDIAESDDRLFFTSFYIAQRKRLLLMLEE
                     GGKPQGGRWSFDRANRRRLPAETSVPVVRRPEEDDIIREARQYVRAHFPDTIGPDEAF
                     GYPTGHAAAAAWLDDFVLTRLGSFGDYEDAISAGHDVLFHSVLSPMLNVGLLSP"
     gene            complement(847..999)
                     /locus_tag="VFZ86_00195"
     CDS             complement(847..999)
                     /locus_tag="VFZ86_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5640739.1"
                     /translation="MSDLQLFIAGVVVAIPAGASMIALVLAAIADGRQNDRFQARQAK
                     KRGDGE"
     gene            complement(992..1183)
                     /locus_tag="VFZ86_00200"
     CDS             complement(992..1183)
                     /locus_tag="VFZ86_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020413824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5640740.1"
                     /translation="MNRRKLRLRAALVILLAAFASFVLSVVLWFTGHENQGIFVGIWV
                     PSILAFGAVALQIVGDRDE"
     gene            complement(1341..2099)
                     /locus_tag="VFZ86_00205"
     CDS             complement(1341..2099)
                     /locus_tag="VFZ86_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008743860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX5640741.1"
                     /translation="MSAADFLIILLAGMGAGTINTVVGSGSLITFPTLVALGYPPLLA
                     NVSNNIGLVPGGVSGAYGYRRELADQGRRLRRLVPASVLGALAGATLLLVLPSSVFDR
                     VVIILIAVALVLVVLQPALSRRLAARRAARPPGHHDATPGVQVLVALAGVYGGYFGAA
                     QGIILISILGIMIDDHLQRLNACKNVLAGSVNATAAIVFVLTTAVEWKVVLAIAVGST
                     IGGQIGARYGRRLDPRALRAFIVVVGLVAIVRLL"
     gene            complement(2376..>3410)
                     /locus_tag="VFZ86_00210"
     CDS             complement(2376..>3410)
                     /locus_tag="VFZ86_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyneurosporene dehydrogenase"
                     /protein_id="HEX5640742.1"
                     /translation="WEDGIRIDTKAPNLEWWYCDADLDDGTGLCMIFATKDGTRPHQP
                     LGPQLEIDITKPDGTRMVRSWYPKIEDFSASKDGCDVRMGDCRFTGDLHEYRVTGHAE
                     ELSADIRLEGITEPWRPESGLLLFGPKDEHFFGWTPFVPFGKVTATYKIGDEVHEATG
                     RGYHDHNWTNTDLGHLIDHWWWARGEVGPYTFLAAHIVATEKYGYTPFHWYMLARDGK
                     VIADDGSKMSFTTHGAEINEKTGKPEPDAVSFDYKDGDTRYELMMTRQKTLVAYTWID
                     FAKGWEKELMKLIRYPGGYMRFLAATTLDCYKAGERVEHYEKSGAFEQIFYQRKIHEE
                     SLERDMAARK"
BASE COUNT          532 a         1129 c         1195 g          554 t
ORIGIN      
        1 gggcgagagc aggccgacgt tgagcatcgg tgacagcacg gagtggaaca ggacgtcgtg
       61 gccggcgctg atcgcgtcct cgtagtcgcc gaacgaaccc agtcgggtca ggacgaagtc
      121 gtcgagccac gcggcggcgg cggcatggcc ggtgggatag ccgaacgcct catccggtcc
      181 gatcgtgtcc gggaagtgcg cccgaacgta ctgcctggcc tcccggatga tgtcgtcctc
      241 ctcgggacgg cgcaccacgg ggacgctggt ctctgcgggc agcctcctgc ggttggcacg
      301 gtcgaagctc cagcgtccgc cctgcggctt gccgccctcc tccagcatga ggagaaggcg
      361 ctttcgctgt gcgatgtaga acgaagtgaa gaacaggcga tcgtcgcttt cggcgatgtc
      421 gcggacgagt gaaggcgggg tcagggagtg cgggtcgtcc acgacaaacg cttcgatccc
      481 ccggcgctcg agagcatctc ccaggcgggc tgcgagccag tcgtcgcagg ggtcgacgta
      541 ccgcaccgag gtgattcgca gccggtgcaa gacctccgca atggcggccg tgtcggccag
      601 ccggcctgcc tcgacgtagt ggaccttcac ctgccgcctg cggaggcccg cggcgaaccg
      661 cttcatcgtg gcgcggtgga ggaccagctt ctgccggtgg aaccgatagt gcgtgaacag
      721 cagtggctct tcgaccagca ccgcccgcga gacgccggcc agcgccggat gctcggcgaa
      781 cagctggtgt ggatagatga gggccgcgcg ggtcacgctt cccggctgtc cgtgacggcc
      841 cccgtgctac tcgccgtcgc cacgcttctt ggcctgccgg gcctggaagc gatcgttctg
      901 ccggccatcc gcgatcgcgg cgaggacgag ggcgatcatg ctggcgccgg cggggatcgc
      961 caccacgacg ccggcgatga agagctggag atcactcatc gcgatcaccc acgatctgca
     1021 gcgcgacggc gccgaacgcc aggatcgacg ggacccagat gcctacgaag atcccctggt
     1081 tctcgtggcc cgtgaaccac agcacgacgg agagcacgaa tgaggcgaac gcggcgagga
     1141 gaatgaccag cgctgcacgg aggcggagct tgcgacggtt cattgggcgg acctccagtc
     1201 agtgcaccac cgttcttgtc cgtcggaaga ggaaccgtct cggcagggcc tcggatcagc
     1261 gctcaagata cccgccgtcc cggagaccgt ccagagacct ccgtttcctc ggcgcgccca
     1321 catgccgttg ccgctgcgtg tcagagcaga cgcacgatcg cgacgaggcc gacgaccacg
     1381 atgaaggcgc gcagggcccg gggatcgaga cggcggccgt agcgggcgcc gatctggccg
     1441 ccgatggtcg agcccacggc gatcgccagc accaccttcc attcgaccgc cgtggtcagg
     1501 acgaagacga tcgcggccgt cgcgttgacc gagccggcga gcacgttctt gcaggcgttg
     1561 aggcgctgca ggtggtcgtc gatcatgatc ccgaggatcg agatgaggat gatgccctgc
     1621 gcggcgccga agtagccgcc gtacacgcct gccagagcca ccagcacctg cacgccgggc
     1681 gtcgcgtcgt gatggcctgg aggccgggcg gcccgccggg cggcgagacg ccgcgagagc
     1741 gccggctgca gcacgaccag cacgagcgcc acggcgatga ggatgatgac gaccctgtcg
     1801 aacacgctgc tcggcagcac cagcagcagg gtcgccccgg cgagcgcgcc gaggaccgac
     1861 gcgggcacca ggcgccgcag acgcctgccc tggtccgcca gttcgcgccg gtagccgtag
     1921 gcgcccgaca ccccgccggg gacgaggccg atgttgttcg agacgttggc cagcagtggc
     1981 gggtagccca gcgccacgag cgtcgggaac gtgatgagcg agccggagcc gacgaccgtg
     2041 ttgatggtcc cggcgcccat gccggcgagc aggatgatca ggaagtcggc agcggacatc
     2101 ggcgggcgcc gttcacgcgg gcgttgtggc tccgatgaac gcgctcaggt gtgtctcagc
     2161 cgtgtccgtc ttggcccttt cggtgtcgag agagacagcc ggcgcacgat ggcggtccca
     2221 cgagccgcgg cgctgccaga caccagttct tcaagcgcgg agggatgccc tgcacgcttt
     2281 gggtgcaggt ggtccgcgcg agcatccagg gtgagtacct tccgagggcg ctgcctatcg
     2341 ccggcgtcgg cgcgtcgtca cgccggtcgt cctcctcact tcctggctgc catgtcgcgc
     2401 tccagcgact cctcgtggat cttgcgctgg tagaagatct gctcgaacgc cccgctcttc
     2461 tcgtagtgct cgacgcgctc gccggccttg tagcagtcca gggtcgtggc cgccaggaag
     2521 cgcatgtagc cgcccgggta gcggatcagc ttcatcagtt ccttctccca gcccttggcg
     2581 aagtcgatcc acgtgtaggc cacgagcgtc ttctggcgcg tcatcatcag ctcgtagcgc
     2641 gtgtccccgt ccttgtagtc gaaggagacc gcgtccggct cgggcttgcc ggtcttctcg
     2701 ttgatctcgg cgccgtgcgt ggtgaagctc atcttggagc cgtcgtcggc gatgaccttg
     2761 ccgtcccggg ccagcatgta ccagtggaac ggcgtgtagc cgtacttctc cgtggccacg
     2821 atgtgcgcgg cgaggaacgt gtaggggccg acctcgccgc gcgcccacca ccagtggtcg
     2881 atgaggtgcc ccaggtcggt gttcgtccag ttgtggtcgt ggtagcccct gccggtggcc
     2941 tcgtggacct cgtcgccgat cttgtacgtg gccgtcacct tgccgaaggg cacgaagggc
     3001 gtccagccga agaagtgctc gtccttgggc ccgaagagca ggaggccgct ctccgggcgc
     3061 cacggctccg tgatcccctc gagccggatg tcggccgaga gctcctcggc gtgccccgtg
     3121 accctgtact cgtgcaggtc gccggtgaat cggcagtcgc ccatgcggac gtcgcagccg
     3181 tccttggagg cgctgaagtc ctcgatcttg ggataccagg atcgcaccat ccgcgtgccg
     3241 tcgggcttgg tgatgtcgat ctcgagctgc ggcccgagag gctggtgcgg gcgggtgccg
     3301 tccttcgtcg cgaagatcat gcacaagccg gtgccgtcgt ccaggtcagc gtcgcagtac
     3361 caccactcca ggttgggcgc tttggtgtcg atgcggatgc cgtcttccca
//
LOCUS       DASUMX010000007         4167 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_2015814, whole genome shotgun sequence.
ACCESSION   DASUMX010000007 DASUMX010000000
VERSION     DASUMX010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4167
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2015814"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="VFZ86_00215"
     CDS             complement(<1..738)
                     /locus_tag="VFZ86_00215"
                     /inference="COORDINATES: protein motif:HMM:NF025865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX5640743.1"
                     /translation="MDVRPEWYDAIGRRTSRRLFDGRSVPPDVRERLEVFCDGASAPA
                     GAPGSPEVQPGDVTAPARACLVDDPAHLLFTGLVGGYGKVTGTPVAAAFIGRAPSGGP
                     ISDDVQTAAGYLGEGLILEATGLGLGTCWIAGSFDRTNADELVDLAPGEQIVAVTPLG
                     YATPRPSNGERLLRTMVKASARLSVEKLAPGVLDGGWPQWAVTAVQAARLAPSGANRQ
                     PWRFRLDGDALVMGRADRLYWTAPIDFG"
     gene            825..1649
                     /locus_tag="VFZ86_00220"
     CDS             825..1649
                     /locus_tag="VFZ86_00220"
                     /inference="COORDINATES: protein motif:HMM:NF017959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HEX5640744.1"
                     /translation="MSAFAYLAAVLVVVWAVLVIVAQLLNPEQSPLAMGMSGLARGRA
                     PWVMKSAFVCRGASALVLLFALPSVLGTAALTLTGMAAFWVWGVGSALLALSDTDMPG
                     EPPSRTGSVHALLALVAYLAGVAGAIMLSVVLLRGDDTAGLGVWALPLALTAAAAMAV
                     QFVAFGAAAREAREAREAAALAAAGSGLAEAPAAAPTAAATAAAPAVAPQLPGVPPQL
                     TAGLAAAPGRPSPAGPGDGPAKWLNDLAGYAGLLQRIFIGLLMAWTLLVALGVAGL"
     gene            complement(2073..2258)
                     /locus_tag="VFZ86_00225"
     CDS             complement(2073..2258)
                     /locus_tag="VFZ86_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5640745.1"
                     /translation="MKKLTVVAVVAAFMAIMAVPALAAPGGTPAAHGVDGKTFGSAVS
                     GLAQSAPGAVAAHVSGK"
     gene            complement(2429..3421)
                     /locus_tag="VFZ86_00230"
     CDS             complement(2429..3421)
                     /locus_tag="VFZ86_00230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HEX5640746.1"
                     /translation="MGWLARAIDAGIEYRKPLADLEDDLFLDTGAHRAKSTKFWVQLA
                     LSSVIAAGGVIGDSTPAVIGAMIIAPLGTPIYGLALAAVVGDRRELRASLRLLLGGIA
                     VNILIGVLIGLVTVNRMPLDVNPQIVGRTAPTVLDLTVAVAVGIAGSFALSRRDVADI
                     LAGVAIAISLVPVLAVVGITLGSLHFDLALGAFILFLTNVAAILVAGALVFSAAGYQR
                     DARDRDERAGRRARRWIIALLVILLIPLGAASAQILRYELLKQDVERATEGWLAGTAW
                     QLDGVEQSGNEFVIKVMGPNSAPPLSELRAAIREQVPEWIPITVIEEAGRRSPL"
     gene            complement(3510..>4167)
                     /locus_tag="VFZ86_00235"
     CDS             complement(3510..>4167)
                     /locus_tag="VFZ86_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5640747.1"
                     /translation="PPAPRPLEAAPVDAGPGVQERARAGAGGRARAPRARIIIAGRPE
                     AQPATTPWRLTWQWVAAAAVTLGLAWLTYSGGGWIPLLSAFDLAVHEFGHLLTFWAPP
                     LLCSLAGSLLQVAAPLALGGYFWWRRDEFAAILMIAWSAENLHNVSVYIGDAEAMLLP
                     LFGDDGSGAGHDWHNILLELNALGATDQLALVVRTCSVMLFVVALGLAALGFSRARRA
                     "
BASE COUNT          561 a         1492 c         1530 g          584 t
ORIGIN      
        1 gccgaagtcg atgggcgcgg tccagtagag cctgtccgcg cgccccatga ccagcgcgtc
       61 gccgtccagg cggaagcgcc agggctggcg gttcgcgccg gagggcgcca gccgggcggc
      121 ctgcacggcc gtgaccgccc actgcggcca gccgccgtcg agcacgccgg gagcgagctt
      181 ctccacgctg agtcgcgccg acgccttgac catcgtgcgc agcaggcgct cgccgttgct
      241 cggccgcggg gtggcgtagc cgaggggcgt gacggcgacg atctgctcgc cgggcgccag
      301 gtccacgagc tcgtcggcgt tcgtcctgtc gaacgagccg gcgatccagc aggtgccgag
      361 gccgaggccg gtggcctcga ggatgagacc ctcaccgagg tagccggccg cggtctgcac
      421 gtcgtcggag atggggccgc cggagggagc gcgcccgatg aaggcggccg ccacgggcgt
      481 cccggtgacc ttgccgtacc ctccgacgag accggtgaag agcaggtgcg cggggtcgtc
      541 gacgaggcag gcgcgggcgg gcgccgtcac atcgcccggc tgcacctccg gtgagccggg
      601 cgcgccggcc ggcgcgctgg cgccgtcgca gaacacctcc agccgctcgc gcacgtccgg
      661 cggcacagac cggccgtcga acagccgccg cgaggtgcgc cggccgatcg cgtcgtacca
      721 ctctggcctg acgtccatgg tggcgatgat aggggggagg ggacggccgg tccagagacg
      781 ggcacgggcg gcgcgcgacc gggtcgttcc tgtagggtta ctccatgagc gccttcgcct
      841 atctggccgc ggtcctggtc gtggtctggg ccgtcctcgt gatcgtcgcg cagctcctca
      901 atccggagca gagcccgctg gccatgggga tgagcgggct cgctcgcggc cgtgctccgt
      961 gggtcatgaa gtccgccttc gtctgccgcg gcgcctcggc cctcgtcctc ctgttcgcgc
     1021 ttccctccgt actgggcacg gccgccctga ccctgacggg catggcggcg ttctgggtgt
     1081 ggggagtggg ctccgccctc ctcgcgctgt ccgacaccga catgccgggt gagccgccgt
     1141 cgcgtaccgg gtccgtccac gcactgctgg cgctggtggc ctacctcgcc ggcgtggccg
     1201 gcgcgatcat gctctcggtg gtgctgctcc gcggcgacga caccgcgggg ctgggcgtgt
     1261 gggccttgcc gctcgcgctc acggcggctg cggcgatggc ggtgcagttc gttgccttcg
     1321 gcgccgcagc gcgcgaggcg cgagaggcgc gagaggcggc cgctttggcc gcggccgggt
     1381 ccgggctcgc ggaggctcct gccgccgccc ccacggcagc cgcgacggca gccgcgccgg
     1441 cagttgcacc gcagctcccc ggcgtgccgc cgcagctcac cgccggcctc gcggccgcgc
     1501 caggacgtcc ttcgccggcg ggtccaggtg atggtcctgc caagtggctg aacgacctcg
     1561 ccggctacgc cggcctgctc cagcgcatct tcatcgggct cctcatggcc tggacgctgc
     1621 tcgtcgcgct cggcgtcgcc ggcctgtagg gatcctcgct ttcggcacgg cactgccgtt
     1681 ccacggacgg tcgcgcgcga ttgattcgtg cgtacggggg cacatcactc gtgcgccgat
     1741 ccgccatgag cagctctcag tccatacgca aggagttctc tcatggagcg tcgctagcag
     1801 cgcccacggg tatctcggac gcgcgcgacc gtgatcgccg cgcgggtgag cggctcatgg
     1861 cagtgaccgg cactcgtgcc ctttgaggtg ccgggccgcc gtggcggccc tcggggaccg
     1921 ggcggcgggc ccggcgccag acgcgttcgc cgcccggaca cacgccaagc gggcggggcg
     1981 tcctgcgtta ccgatcaccc cgcccgctct gtgccttgcg tgccttcgat cgccggccgt
     2041 gacttgtgcc tgacgatgct cggcgctacg tcttactttc cggaaacgtg cgcggcgaca
     2101 gcgccaggag cgctctgcgc caggccgctt acggctgagc cgaaggtctt gccgtcgacg
     2161 ccgtgagccg ccggcgtgcc gccgggagct gcaagcgctg gtaccgccat gatcgccatg
     2221 aacgcggcaa cgactgcgac tacggtgagc ttcttcatcc gggcactccc cactgtctcg
     2281 tttgcacgat cgccgctggg gcggccgtgc cctggaagcc ttctcgatgg agaagtaact
     2341 tcgctgcagt gatacccagc cgtggagata gcgaaccctc gtgaactgcg ctcgcgccag
     2401 tcacgcgatg tgggcgacgg ccggccgcct acagcggcga cctgcggccg gcctcctcga
     2461 tcacggtgat cgggatccac tccgggacct gctcgcggat cgccgctctg agctcggaga
     2521 gcggcggcgc cgagttcggc cccatcacct tgatcacgaa ctcgttgccg gactgctcga
     2581 ccccgtccag ctgccaggcg gtgccggcca gccatccctc ggtggctctc tccacgtcct
     2641 gcttcaggag ctcgtagcgc aggatctgcg ccgacgcggc gcccagcggg atcaggagga
     2701 tgacgaggag cgcgatgatc cacctcctcg cccggcgccc ggcacgctcg tcgcggtcgc
     2761 gggcgtcgcg ctggtagccg gcggccgaga acacgagggc gccggcgacg aggatcgcgg
     2821 cgacattggt gaggaacagg atgaaggcgc ccagcgccag atcgaagtgc agcgagccga
     2881 gggtgatgcc gacgaccgcg agcacgggca cgagcgagat ggcgatggcc acgccggcca
     2941 ggatgtcggc gacgtcgcgg cgggagaggg cgaacgagcc ggcgatgccg acggcgaccg
     3001 cgaccgtgag gtcgaggacc gtcggcgccg tgcgtcccac gatctgcggg ttcacgtcga
     3061 gcggcatgcg gttcacggtg accaggccga tgagcacgcc gatgaggatg ttcacggcga
     3121 tgccgcccag gagcaggcgc agcgacgcgc gcagttctcg ccggtcgccg acgacggccg
     3181 cgagcgcgag cccgtagatg ggcgtgccga gcggggcgat gatcatcgcc ccgatcaccg
     3241 ccggtgtgga atccccgatc acgccgccgg cggcgatcac gctcgagagc gccagctgca
     3301 cccagaactt ggtggacttg gcgcggtggg ctccggtgtc gaggaacagg tcgtcttcga
     3361 gatccgccag cggtttgcgg tactcgatcc ccgcgtcgat cgctctcgcc agccagccca
     3421 cgcacgcctc ctcgtaccgc cgtctcgtgt cgtgcgggag tatacgagag gtgaggctct
     3481 cagcgagagg ctcggctctc cgcgagaggt caggctcgcc gcgcccgcga gaagcccagg
     3541 gcggcgagcc ccagcgccac cacgaagagc atgaccgagc aggtcctgac cacgagcgcg
     3601 agctggtcgg tggccccgag cgcgttcagc tcgagcagga tgttgtgcca gtcgtggccc
     3661 gcgccggacc cgtcgtcgcc gaagagcggc agcagcatcg cctccgcgtc accgatgtac
     3721 acggacacgt tgtgcaggtt ctccgccgac cacgcgatca tgaggatcgc cgcgaactcg
     3781 tcgcggcgcc accagaagta gccgcctagg gccagcggcg ccgcgacctg cagcagcgag
     3841 ccggcgagcg agcagagcag cggcggcgcc cagaacgtga gcaggtggcc gaactcgtgc
     3901 accgcgagat cgaacgccga gagcagcggg atccagccgc cgccggagta ggtgagccag
     3961 gccaggccga gcgtgacggc cgccgccgcc acccactgcc acgtgaggcg ccagggggtg
     4021 gtcgccggct gcgcctccgg ccggccggcg atgatgatgc gcgcgcgggg agcgcgggcg
     4081 cgcccccccg cgccggctcg cgcgcgctcc tgcacgccgg gtccagcgtc caccggcgcc
     4141 gcttccagcg gtcgcggcgc cggcggc
//
LOCUS       DASUMX010000008        13131 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1440743, whole genome shotgun sequence.
ACCESSION   DASUMX010000008 DASUMX010000000
VERSION     DASUMX010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 13131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13131
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1440743"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            97..264
                     /locus_tag="VFZ86_00240"
     CDS             97..264
                     /locus_tag="VFZ86_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEX5640748.1"
                     /translation="MGGTADESKGRLKKAAGELTGNERLKREGDVDKASGKVKKATDK
                     VADKAKDAFRK"
     gene            296..439
                     /locus_tag="VFZ86_00245"
     CDS             296..439
                     /locus_tag="VFZ86_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5640749.1"
                     /translation="MTTETFPKISRFLESRRSPRRDSSDKTGGVPVNTMFDLDVGPRF
                     LRR"
     gene            complement(611..686)
                     /locus_tag="VFZ86_00250"
     tRNA            complement(611..686)
                     /locus_tag="VFZ86_00250"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(651..653),aa:Thr,seq:cgt)
     gene            complement(747..1115)
                     /locus_tag="VFZ86_00255"
     CDS             complement(747..1115)
                     /locus_tag="VFZ86_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5640750.1"
                     /translation="MDPLIRSLDFVAVPSTDAARTRAFYVDTLGLRPDDRAQFEFWVG
                     DTCFGIWEPKAMGMPFAPVTAGHIALHVDDVAVARAALEEKGVQFAGDTFDTGVCHMA
                     LFADPDGNALMLHHRYSPRV"
     gene            1363..1767
                     /locus_tag="VFZ86_00260"
     CDS             1363..1767
                     /locus_tag="VFZ86_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5640751.1"
                     /translation="MRRPLATLTIGIVAVAVAVMAFAGVTAPRSGATASLCHTTTLSI
                     KAPSSVKAGGRITVTGVEAKRPAHTVKATLQSRRATARRWINGASVNLSASGAYSLKW
                     RAPARKGKYKIRVRVTYSGTANTSVVRTVTVK"
     gene            2151..2819
                     /locus_tag="VFZ86_00265"
     CDS             2151..2819
                     /locus_tag="VFZ86_00265"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5640752.1"
                     /translation="MDGVATEIRVLLVDDHAIERQGVRSLLDAQPDIQVVAEAGDGLE
                     ALPLVGQCHPDVVITETIMPRMNGLELTGQLLRRYPDLKVLVLTRNDREDCVLRLVQA
                     GAGGYLLKTVDRIDLVAAVRAVMHGSRVLQPTALETVLDDYLQRVREPSARRYAVELT
                     AREREVLKLIAEGNTNQDIADLLCLSRKTVETHRSNIMDKLDMHKVTDLVKYAIREGL
                     VGLE"
     gene            2996..3127
                     /locus_tag="VFZ86_00270"
     CDS             2996..3127
                     /locus_tag="VFZ86_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5640753.1"
                     /translation="MPEEVIVFAIEGADVETFGEELSPPATAVAEQVLGLVRREVGA"
     gene            3124..3996
                     /locus_tag="VFZ86_00275"
     CDS             3124..3996
                     /locus_tag="VFZ86_00275"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HEX5640754.1"
                     /translation="MSVGVVAFVTHPLPELRTALRGIRETAPPDAQLAVVATDLGDQD
                     ATYLLRQYLRGRIHALDFERAGARGGHCGLDRAYQLVHGEYLARVDDTLEFQPGWLER
                     AVSILQADPSLGCLSLVPPADYHRGRGRPRTVHVEPIEVDHLELRCFIAPRELVERHE
                     CELMAVQPGGCGFLDYLRGDGRRLAFLPGLVSPLGVIEGPTGLDSCSVEAELPVHEGA
                     SGAMQRLEQAYALGDDILLTCLSCGGSELEVLAARIRFCERHQVATGFWYELRCPECG
                     ELHYKDDFQFRCPE"
     gene            3993..6572
                     /locus_tag="VFZ86_00280"
     CDS             3993..6572
                     /locus_tag="VFZ86_00280"
                     /inference="COORDINATES: protein motif:HMM:NF013468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HEX5640755.1"
                     /translation="MSGQALSAARISVRGVVQGVGFRPFVYSLAVRYGLRGWVLNTSA
                     GVEVLVEGPPDAVAAFCEALPGEAPPRSHIATYEVRAVPAGAAADGPEAGDAFVILES
                     QADPDAYQLVSPDIATCEDCRHELFDPADRRHEYPFTNCTNCGPRFTIIEGLPYDRER
                     TTMRRFPLCPACRREYEDPADRRFHAEPNACPVCGPRVRLLRLAGPGAGSGEEVELAA
                     GAEGDPAGPIRAAAELLGAGEILAVKGLGGFHLACDATDGDAVRRLKLRKRRPDKPLA
                     VMFAGVDELRRHCRVTSAEEALLASPEHPVVLLEWREIGPDGEPGPELGAGGRPQHDA
                     FTDEEAPVDPEVAVRQRYLGAMLPYTPLHVLLLRAAGRPLVMTSGNLAEEPIVKGWEE
                     IDRLTQVADAYLVHDREIAVRYDDSVALVRAERPRLIRRSRGYAPFPVTLPRSLPQTL
                     AAGAELKNTFCLTRDANAFLSQHIGDLENLETLEHYEASIEVYRKLFRLDPEVVAYDL
                     HPEYLATKYALALPQPEKVAVQHHHAHIAARLVEHGREEAVIGVSLDGLGYGDDGRLW
                     GGEVLVCDLLGYRRVAHLEELPLPGGAAAITHPWRTGLGWSYALLGEEGLARATRLLG
                     RRPDGPDEAERAAVVRQIERGVNVPLTTSSGRLFDAVAALAGVRHTISYEGQAAIELE
                     MMASGQMLRAAGAAPYAWSLDGDPGAAASAPRAGAAEWAAARAAVARAASGLHLVPDV
                     PLEPAPAVVRLAPLMAGVIADLEAGAAPGAVGARLHVTLAAVVLDLCRRVRAASGLTA
                     VALSGGVFQNRLLADLCSDKLAGDGFQVLGAGLVPVNDGGVSLGQAAVAGYTVLERRG
                     ELA"
     gene            6615..6881
                     /locus_tag="VFZ86_00285"
     CDS             6615..6881
                     /locus_tag="VFZ86_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014779942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HEX5640756.1"
                     /translation="MCLAVPMRITALDGVRATIAVEGLEQECSVMLVPDAGVGDYVLV
                     HAGFALSVLDEAEAQETYELLREIVALDEDEDEGAAAADSDAGG"
     gene            6868..7935
                     /gene="hypD"
                     /locus_tag="VFZ86_00290"
     CDS             6868..7935
                     /gene="hypD"
                     /locus_tag="VFZ86_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009440645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HEX5640757.1"
                     /translation="MPAAEDLGRLAAELRSEAPPGLTFMEVCGTHTMAIARYGLRQLL
                     PEGVRLISGPGCPVCVTAMADLDRVLALARLPEVTLVTFGDLIRVPASRGTLAGERAA
                     GADVRVVYSPRDAVRIAAEEPGRQVVFAGIGFETTAPTTAAALLEARAEGLANFSLLS
                     LHKTMPLPLRALLDLGETPISGFLLPGHVSVITGTACYGFLAREYGIAGVVAGFEARD
                     VLEALLMLVRLPRPEIAIQYTRAVRPEGNLVAQRLLDQVFAPSDADWRGLGVIPGSGL
                     ALREEFADVDAARRFAVDPGPTLEPPGCRCGEVLRGVIDPAACPLFGTRCTPEDPVGA
                     CMVSSEGACAARYRYRGLDEA"
     gene            7925..8971
                     /gene="hypE"
                     /locus_tag="VFZ86_00295"
     CDS             7925..8971
                     /gene="hypE"
                     /locus_tag="VFZ86_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004381982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HEX5640758.1"
                     /translation="MKPDHPGRTGDRVLLGHGSGGKLYRDLVKDVFVRAFANPVLERL
                     DDAAALSPSGRIAFTTDAHVVQPLTFPGGDIGRLSVAGTVNDLATAAARPLALAAAFI
                     IEEGFALDDLRAVCASMAATAREARVPIVTGDTKVVERAAADGLFITTTGVGEIVVGH
                     ELGSGAVRDGDAVILSGSIGDHAVAILAARGEFAFKADARSDCAPLWDLVDAAVAAAR
                     AGGGEEAIRFLRDPTRGGLTTVLAELAEESGLGIEVEEEAIPVTPAVRSVCDLLGYDP
                     LTLANEGKMVLVTAAEAADAVVAAVRATKYGGEARRIGFVTAAHPGRVALRTPFGTRR
                     VLDMPVGELLPRIC"
     gene            9111..9368
                     /locus_tag="VFZ86_00300"
     CDS             9111..9368
                     /locus_tag="VFZ86_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5640759.1"
                     /translation="MDLDAELAGMDQEQLKEALSQAELQLEDVQEERDFTLGQTGVHI
                     GANELERLRRAWETDERRLRDRMAAISARLAGLQAGPGPEA"
     gene            9394..10062
                     /locus_tag="VFZ86_00305"
     CDS             9394..10062
                     /locus_tag="VFZ86_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX5640760.1"
                     /translation="MKGREYKSAVYEQFALIGKALCSGPRLEILDVLQQAERTVEVLA
                     AEVGLSVANTSHHLQVLKRGRLVESERRGQHVVYRLADPAVGDALRAVRVLGERRLAE
                     IERVTAEFLGDREGMEPVEEDALVMRVANGDCVVLDVRPSEEYAAGHLAGAISLPLEQ
                     LERRLAELPRDRPIVAYCRGPYCVLAVNAVEALRRHGFDAVRAPEGVREWRELGLEIT
                     DTNG"
     gene            10076..10426
                     /locus_tag="VFZ86_00310"
     CDS             10076..10426
                     /locus_tag="VFZ86_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005966802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HEX5640761.1"
                     /translation="MKYLFILNDPAYGTERSYNGLRLAMTLLTQEPEAEVTVFLMGDA
                     VTCAKAGQKTPDGYYNLERMLKPIVRKGEVLLCGTCMDARAMTETEICEGTRRSSMSE
                     LTAKTADADKVLVF"
     gene            10431..11558
                     /locus_tag="VFZ86_00315"
     CDS             10431..11558
                     /locus_tag="VFZ86_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013504967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HEX5640762.1"
                     /translation="MIYLDHNASTPIAPQVREAMLPFLAEHHGNPSSAHWAGAPAAQA
                     VADAREQLAALLGCSPHEVVFTSGGTEANNTALKGVVEAAGAERPHIVTTAVEHPAIV
                     EPCRYLERHGVDVTVVGVDATGLVDPDDIAAAIRPETVLVSVMHANNEVGTVEPIAEI
                     SRITREAGVLLHSDAAQSVGKVPTRVDDLGVDLLSVAAHKFYGPKGVGALYVREGTRL
                     EPFMHGAGHEDGRRAGTENVLLDVALGAAAELAGDLAWTLGVRDLRDWLWLQLQDRWG
                     ERVVLNGHPTARLPNTLNVSFLGRDGRAVLAAMPEVAASTGSACHSGGTEPSPVLAAM
                     GVPREVGAGAIRLSLGRKTTRAELETLLERLAEVLSDGGER"
     gene            11555..12370
                     /locus_tag="VFZ86_00320"
     CDS             11555..12370
                     /locus_tag="VFZ86_00320"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HEX5640763.1"
                     /translation="MSATRRLDRLRFGTFLAPNIMPVYEAVTEEVGRRLGVETELFIE
                     TDYEACAKDDNEVCFVCSLPYVDYERRGLSPAVPVAAPVLVGDRYAGKPVYFSDVIVH
                     RDSPFRSFLDLRGHSWAYNEPLSHSGYGITRYHLLEVGETHGFFSEVIEAGFHETSMR
                     LVAAGEVDASAIDSQVLAVALRDDPSLARSLRVIDALGPSTIQPVAVSRRVPARQRDE
                     IREVLTTMHEDPPMRERLALGMVERFVPADASSYDDIRMMLEACEAAGFMEVR"
     gene            complement(12393..12468)
                     /locus_tag="VFZ86_00325"
     tRNA            complement(12393..12468)
                     /locus_tag="VFZ86_00325"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12433..12435),aa:Thr,seq:ggt)
     gene            complement(12470..12554)
                     /locus_tag="VFZ86_00330"
     tRNA            complement(12470..12554)
                     /locus_tag="VFZ86_00330"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12518..12520),aa:Tyr,seq:gta)
     gene            12966..13042
                     /locus_tag="VFZ86_00335"
     tRNA            12966..13042
                     /locus_tag="VFZ86_00335"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13000..13002,aa:Met,seq:cat)
BASE COUNT         1887 a         4682 c         4710 g         1852 t
ORIGIN      
        1 acgacgaggg tcccagggat ggcaacgggg atgccaccac ctctgggtct ctgcgggaat
       61 gtgtgtacca ggccgaatca cagaggaggt gcttccatgg gaggcacggc agacgagagc
      121 aagggtcgtc tcaagaaggc cgccggggag ttgaccggca atgagcggct caagcgagaa
      181 ggagacgtcg acaaggccag cggtaaggtc aagaaggcca ccgacaaggt cgcggacaag
      241 gcgaaggacg cattccgcaa gtgatccacc tgcgatacca cacactggaa gcgagatgac
      301 aaccgaaacg ttccccaaga tcagccggtt cctcgaaagc aggcgatcgc cgagaaggga
      361 ctcctccgac aagacagggg gcgtcccggt caacacgatg ttcgacctcg acgtgggccc
      421 acggttcctc cgccgctgac gagcaccgcg agcactccct tcggccgccg cgttcccaag
      481 aggcgcggcg gccttcgttc gccgtgaggg ggcgatcggg aggggagggt cggcgcgagt
      541 cgcgaggccc ttggacggcg ggtcacgagg atcgtcgatg gggctcggcg gactttgcag
      601 gaagatggag tggagccgac gcggggaatc gaaccccgga ccccatcatt acgagtgatg
      661 tgctctgcca gctgagctac gtcggcccaa atgaagccat ggcattgtag gtcacggcgc
      721 gggcgcgcgc aaagcgccgc cgtcgctcag acgcgcgggg agtaccggtg gtgcagcatc
      781 agcgcgttgc cgtccggatc ggcgaagagg gccatgtggc agacgccggt gtcgaaggtg
      841 tcgccggcga actggacgcc cttctcctcg agcgcggctc tggcgacggc cacgtcgtca
      901 acgtgcaggg cgatgtggcc ggccgtgacc ggagcgaacg gcatgcccat cgccttgggc
      961 tcccagatgc cgaagcacgt gtcgccgacc cagaactcga actgggcgcg atcgtcggga
     1021 cgcaggccaa gggtgtcgac gtagaacgcg cgtgtgcgcg ccgcgtcggt ggacgggacc
     1081 gccacgaagt cgagggagcg gatgagaggg tccacggcaa cctccgtcgt cgaaagcgaa
     1141 cgtacgttcg atgatgcttt gccgccgccg cgttgtcaag gctgggcgtt gtgatgccca
     1201 ccgtcttctt cacaccagct caacacccac gcaacgtttg gcctgtgccg aaacgctaaa
     1261 ctgccctcac tccgcctatc ggtggaggag cggcagcctc tgcgcgccgc gccgcggtcg
     1321 cccagggcgg ccggcgggtc cgcaggacga ccaaggagga ccatgaggag accactcgcg
     1381 acactcacga tcggtatcgt ggcggtggcg gtcgccgtga tggcgttcgc cggcgtcacc
     1441 gcgccgaggt cgggcgcgac cgcgtccctg tgccacacca ccacgctgtc gatcaaggct
     1501 ccgtcgagcg tgaaggcggg agggcggatc accgtgaccg gcgttgaggc caagcggccc
     1561 gcgcatacgg tgaaggcgac gctgcagagc cgtcgcgcga ccgccaggcg gtggatcaac
     1621 ggcgcctccg tcaacctttc cgcgagcggc gcctactcgc tcaagtggag ggctccggcc
     1681 aggaagggca agtacaagat ccgcgtgcgt gtcacgtatt ccggtaccgc caacacctcc
     1741 gtcgtgagga cggtgacggt gaagtaggcc cgggcggtgc gcaaggaccg gcggcagtct
     1801 cggccagctg tgtagggccg gcggcctcgc ccgagggcga cgccgccggc ccttcgcctg
     1861 tcccgcctgt cccgcggcag gctctggtgc gtcgatggtg agcagcggcc tcggagtccc
     1921 ccgccagatg gcatcctccc ttcgggggat gcccggaatg gtccggatgg atcatgtgaa
     1981 cgcgcgcaca tgtccactat gtcctgcgct tgcgctcctg tcttccgtcc cggagaggag
     2041 cgtccattgc ccccatccgg tgcagaccct agaatccaag gtatgaagca ccggaccgag
     2101 ggcggggtgg agcctgcgcc cgcggtcggg tggaagggtg tgtaggcaga gtggacgggg
     2161 tcgccaccga gatccgcgtc ctgctcgtgg acgatcacgc catcgagcgt cagggcgtgc
     2221 gttccctgct cgacgcgcaa ccggacatcc aggtcgtcgc cgaggccggc gacggcctcg
     2281 aggcgcttcc cctcgtcggc cagtgccatc ccgacgtggt catcaccgag acgatcatgc
     2341 cgcgcatgaa cggcctcgag ctcaccggcc agctcctgcg gcggtatccg gacctcaagg
     2401 tgcttgtcct gacgcgcaac gaccgcgagg actgcgtgct gcggctcgtg caggccggcg
     2461 ccggaggcta tctgctcaag acggtcgaca ggatcgacct ggtcgcggcc gttcgcgcgg
     2521 tcatgcacgg cagccgcgtc ctgcagccca cggcgctcga gacggtgctg gacgactacc
     2581 tgcagcgcgt ccgcgagccc tcggctcgcc ggtacgcggt cgagctcacg gcgcgcgagc
     2641 gcgaggtcct caagctgatc gccgagggca acaccaacca ggacatcgcc gacctgctgt
     2701 gcctcagccg caagacggtc gagacgcacc gcagcaacat catggacaag ctggacatgc
     2761 acaaggtcac cgacctggtc aagtacgcga tccgcgaggg tctggtcggc ctggagtgag
     2821 gttcgtgggg ggtgctcgtc gccgggcttc gtctgctcga acttgtgcgg cgattcaccc
     2881 gcggtatcat tatctgtgcg ctgaagagcc ccgccgaggc cgggctgagc cgggcgaggt
     2941 cgtccgccat gggctcgaca gtgcgctgga gctcggccgc cagctcggct gcgacatgcc
     3001 tgaggaggtc atcgtgttcg ccatcgaggg ggcagacgtg gagacgttcg gcgaggagct
     3061 ctcgccgccc gccaccgccg tcgccgaaca ggtcctcggc ctggtccgcc gggaggtggg
     3121 cgcgtgagcg tcggcgtcgt cgccttcgtc acgcacccgc tgccggagct gcgcacggcg
     3181 ctccgcggca tccgcgagac cgcgccgccg gacgcgcagc tcgccgtggt cgccaccgac
     3241 ctcggggacc aggacgccac ctaccttctg cgccagtacc tgcgcgggcg catccacgct
     3301 ttggacttcg agcgcgccgg cgcacgcggc ggccactgcg gtctggaccg ggcctatcag
     3361 ctcgtgcacg gcgagtacct tgcgcgtgtg gacgacacgc tcgagttcca gcccgggtgg
     3421 ctcgagcgcg ccgtttcgat cctgcaggcg gacccttcgc tcggctgtct ctcgctggtg
     3481 ccgccggccg actaccaccg cggccgcggc aggccgcgga cggtgcatgt cgagccgatc
     3541 gaggtcgacc atctcgagct gcgctgcttc atcgcgccgc gcgagctggt cgagcgccac
     3601 gagtgcgagc tcatggccgt gcagcccggc gggtgcggct tccttgacta cctgcgcggc
     3661 gacggccggc ggctggcgtt cctgcccggc ctcgtcagcc cgctcggggt catcgaaggg
     3721 cctaccggcc tcgactcctg cagcgtcgag gcggagctcc cggtgcacga gggagcctcg
     3781 ggcgccatgc agcgtctcga gcaggcctac gccctcggcg acgacatcct gctcacctgc
     3841 ctctcgtgtg gcgggtccga gctcgaggtg ctggcggccc gcatccgttt ctgcgagcgg
     3901 caccaggtcg ccaccggctt ctggtacgag ctgcgctgcc ccgagtgcgg cgagctccac
     3961 tacaaagacg acttccagtt ccgctgcccg gaatgagcgg gcaggcgctg tccgcggcgc
     4021 gcatcagcgt ccgtggcgtg gtccagggcg tcggcttccg cccgttcgtg tactccctcg
     4081 ccgtgcgcta cggcctgcgc gggtgggtgc tgaacacgtc cgccggcgtg gaggtgctcg
     4141 tggagggccc gccggacgcg gtcgccgcgt tctgcgaggc gctgccgggg gaggcgccgc
     4201 cccgcagcca catcgccacg tacgaggtgc gcgcggtgcc ggccggcgcg gccgcggacg
     4261 gcccggaggc cggcgacgcg ttcgtcatcc tcgagagcca ggccgacccg gacgcctacc
     4321 agctggtctc ccccgacatc gcgacctgcg aggactgccg ccacgagctg ttcgatccgg
     4381 cggaccgccg ccacgagtac cccttcacca actgcaccaa ctgcgggccc cggttcacga
     4441 tcatcgaggg gctgccctac gaccgcgagc gcacgaccat gcgacgcttc cccctctgtc
     4501 ccgcgtgccg ccgcgagtac gaggacccgg ccgaccggcg cttccacgcc gagcccaacg
     4561 cgtgcccggt ctgcgggccg cgggtacgcc tgctgcggct ggcggggccc ggcgcgggct
     4621 ccggcgagga ggtggagctc gccgccggcg ccgaaggcga cccggcgggc cccatccgcg
     4681 cggccgccga gctgctgggc gccggcgaga tcctcgccgt caagggcctc ggcggcttcc
     4741 acctcgcctg cgacgccacc gacggcgacg cggtgcgccg cctcaagctg cgcaagcggc
     4801 gcccggacaa gccgctcgcc gtcatgttcg ccggcgtcga cgagctccgc aggcactgcc
     4861 gtgtgacctc ggcggaggag gcgctgctcg cgtcgcccga gcaccccgtg gtgctgctcg
     4921 agtggcgcga gatcggcccg gacggtgaac cggggccgga gctcggcgcc ggcggccggc
     4981 cgcagcacga cgccttcacg gacgaggagg cgccggtcga ccccgaggtc gcggtccgcc
     5041 agcggtatct cggcgcgatg ctcccgtaca ccccgctgca cgtcctgctg ctgcgcgccg
     5101 ccgggcggcc gctggtgatg accagcggca acctcgccga ggagccgatc gtcaagggct
     5161 gggaagagat cgaccgcctg acgcaggtcg cggacgcgta cctggtccac gaccgtgaga
     5221 tcgccgttcg ctacgacgac tccgtggcgc tggtgcgcgc ggagcgcccg cggctgatcc
     5281 gccgctcgcg cggctacgcc ccgttcccgg tgacgctgcc acggtcgctt ccgcagacgc
     5341 tggccgcggg cgcggagctc aagaacacgt tctgcctgac gcgcgacgcc aacgccttcc
     5401 tgagccagca catcggcgac ctcgagaacc tggagacgct ggagcactac gaggcgagca
     5461 tcgaggtgta ccgcaagctg ttccgcctcg acccggaggt cgttgcctac gacctgcacc
     5521 cggagtacct ggccaccaag tacgcgctgg cgctgccgca gcccgagaag gtggccgtgc
     5581 agcaccacca cgcgcacatc gcggcgcggc tggtcgagca cggccgggag gaggccgtca
     5641 tcggggtgag cctggacggc ctcggctacg gcgacgacgg ccgcctgtgg ggcggcgagg
     5701 tcctggtgtg cgacctcctg gggtaccgca gggtcgcgca cctggaggag ctgccgctgc
     5761 ccggcggcgc cgcggcgatc acgcatccct ggcgcacggg tctcggctgg agctacgcgc
     5821 tcctcggcga ggagggtctc gcgcgggcga cccgcctgct cgggcggagg cccgacgggc
     5881 cggacgaggc ggagcgcgcc gccgtcgtgc ggcagatcga gcgtggcgtc aacgtcccgc
     5941 tgacgacgag cagcgggcgg ctgttcgacg ccgtcgccgc cctcgccggc gtgcgtcaca
     6001 cgatcagcta cgagggccag gcggcgatcg agctcgagat gatggcgtcc ggccagatgc
     6061 tgcgggcggc cggcgcggcc ccatacgcgt ggtcgctgga cggcgacccg ggcgccgccg
     6121 ccagcgcgcc gcgtgccggc gcggcggagt gggcggccgc tcgcgccgcc gtggcccgcg
     6181 ccgcctccgg cctccacctc gtcccggacg tgcccctcga gcctgcgccg gccgtggtgc
     6241 gcctcgcccc gctgatggcc ggcgtgatcg ccgacctgga ggcgggcgcc gcgcccggcg
     6301 ccgtcggggc gcggctgcac gtgaccctcg ccgccgttgt gctcgacctc tgtcgccgcg
     6361 tccgcgccgc gtcggggctc acggccgtgg cgctctccgg cggcgtgttc cagaaccggc
     6421 tgctggccga tctgtgctcg gacaagctgg ccggcgacgg cttccaggtg ctcggcgccg
     6481 gcctcgtccc ggtcaacgac ggcggcgtct ccctggggca ggccgcggtc gcgggctata
     6541 ctgtgctcga gcgccgcggc gagctcgcct gatcgcggcg cgccggccag cgtagagacc
     6601 aaaggacccc gcccatgtgc ctcgccgtcc cgatgcgcat caccgccctc gacggcgtgc
     6661 gcgcgaccat cgcggtcgag ggcctcgagc aggagtgcag cgtgatgctc gtcccggacg
     6721 cgggcgtggg cgactacgtg ctcgtgcacg ccggcttcgc cctcagcgtg ctcgacgagg
     6781 cggaggcgca ggagacctac gagctgctgc gcgagatcgt cgcgctggac gaggacgagg
     6841 acgagggcgc cgccgccgcc gacagtgatg ccggcggctg aggacctcgg ccggctcgcc
     6901 gccgagctgc gctcggaggc gccccccggc ctcaccttca tggaggtctg cggcacccac
     6961 accatggcga tcgcccgcta cgggctgcgc cagctgctgc cggagggcgt gcgcctgatc
     7021 agcggcccgg gctgcccggt ctgcgtcacc gccatggccg acctcgaccg cgtgctggcg
     7081 ctggcccgcc tgcccgaggt gacgctggtg acgttcggcg acctcatccg ggtgccggcg
     7141 tcgcgcggca cgctggccgg ggagcgcgcc gccggcgccg acgtccgcgt cgtctactca
     7201 ccgcgcgacg cggtgcgcat cgcggccgag gagcccggcc gtcaggtcgt cttcgccggc
     7261 atcggcttcg agaccaccgc cccgaccacg gccgccgccc tcctcgaggc gcgcgccgaa
     7321 ggcctcgcca acttcagtct cctcagcctg cacaagacca tgccgctgcc gctgcgcgcg
     7381 ctcctcgacc tgggggagac gcccatcagc gggttcctcc tgccgggcca cgtcagcgtc
     7441 atcacgggga cggcctgcta cgggttcctc gcgcgggagt acgggatcgc cggcgtggtg
     7501 gcggggttcg aggcgcgcga cgtgctggag gcgctgctca tgctggtgcg cctcccccgg
     7561 ccggagatcg ccatccagta cacgcgcgcg gtgcggccgg aggggaacct ggtggcgcag
     7621 cgcctgctgg accaggtgtt cgcgccgagc gacgccgact ggcgtggcct cggcgtgatc
     7681 cccgggtccg gcctcgcgct gcgggaggag ttcgccgacg tggacgccgc gcggcggttc
     7741 gccgtcgacc cgggacccac gctggaaccg cccgggtgcc gctgcggcga ggtcctgcgc
     7801 ggcgtcatcg acccggcggc ctgcccgctc ttcggcacgc gctgcacccc ggaggacccc
     7861 gtcggcgcgt gcatggtcag cagtgagggc gcctgtgcgg cccgctaccg ctaccggggg
     7921 ctcgatgaag cctgaccacc ccggccggac cggcgaccgc gtcctcctcg gccacggcag
     7981 cggcggcaag ctgtaccgcg acctcgtcaa agacgtcttc gtgcgcgcgt tcgccaaccc
     8041 ggtgctcgag cgcctggacg acgcggccgc gctctcgccc tccggccgca tcgccttcac
     8101 cacggatgcg cacgtggtgc agccgctcac gttcccgggc ggcgacatcg gccggctgtc
     8161 cgtggcgggc acggtgaacg atctggcgac ggcggcggcg cggccgctgg cgctcgccgc
     8221 cgccttcatc atcgaggagg gcttcgccct ggacgacctg cgcgccgtct gcgccagcat
     8281 ggcggccacg gcgcgcgagg cccgcgtccc catcgtcacc ggcgacacca aggtcgtgga
     8341 gcgcgccgcc gccgacggcc tcttcatcac gaccacgggc gtgggggaga tcgtcgtggg
     8401 gcacgagctc ggctccggtg ccgttcggga cggcgacgcc gtcatcctca gcggctccat
     8461 cggcgaccac gccgtcgcca tcctcgcggc gcgcggcgag ttcgccttca aggccgacgc
     8521 gcgcagcgac tgcgcgccgc tctgggacct cgtcgacgcc gccgtcgccg cggcgcgcgc
     8581 cggcggtggg gaagaggcca tccgctttct ccgcgacccg acccgcggcg gcctcaccac
     8641 cgtgctggcc gagctcgccg aggagtcggg cctgggcatc gaggtcgagg aggaggccat
     8701 cccggtgacg ccggcggtcc gctccgtctg cgacctgctc ggctacgacc cgctgacgct
     8761 ggccaacgag ggcaagatgg tgctggtgac ggcggcggag gcggcggacg ccgtggtggc
     8821 cgccgtccgt gcgacgaagt acggaggcga ggcgcggcgg atcggcttcg tcaccgccgc
     8881 ccaccccggc cgcgtcgccc tgcgcacgcc cttcggcacg cgccgcgtgc tggacatgcc
     8941 ggtgggtgag ctgctgccgc ggatctgctg acgtccgctc cgagcggctt cgccgtcatc
     9001 gcccgagccc ggcgcgtgcg aggtcgctcc accatcaacg aggttgacgg tggccatctc
     9061 gtgtcaaata ctcaagtaaa cgtttgaata ctcgcggagg atggcggcgt atggacctgg
     9121 acgcggaact ggcggggatg gatcaggagc agttgaagga ggccctgagt caggccgagc
     9181 tccagcttga ggacgtccag gaggagaggg acttcacact cggccagacc ggtgtgcaca
     9241 tcggcgccaa tgagctcgag cgcctgcgga gagcctggga gacggacgag cggcgtctgc
     9301 gggatcggat ggccgcgatc agcgcccgtc tcgccgggct gcaggcgggc cctggccccg
     9361 aggcctgatg agcgtcttcg gaaggtggtg cgcgtgaagg gtcgcgagta caagagcgcc
     9421 gtctacgagc agttcgccct gatcggcaag gcgctgtgca gcgggccgag gctcgagatc
     9481 ctggacgtcc tccagcaggc cgagcggacg gtcgaggtgc tcgccgccga ggtcggcctc
     9541 tcggtcgcca acacctcgca ccacctgcag gtgctcaagc gcggccgcct cgtggagagc
     9601 gagcgccgcg gccagcacgt cgtctaccgc ctcgccgacc cggcggtcgg cgacgccctg
     9661 cgcgccgtgc gcgtgctcgg cgagcgtcgc ctcgcggaga tcgagcgcgt gaccgccgag
     9721 ttcctgggcg accgcgaggg catggagccc gtggaggagg acgcgctcgt catgcgagtc
     9781 gcgaacggcg actgcgtggt gctcgacgtg cggccttcgg aggaatacgc cgctggtcac
     9841 ctcgccggcg cgatctcgct gccgctcgag cagctcgagc gacgcctcgc cgagctcccc
     9901 cgcgaccgcc ccatcgtcgc ctactgccgt ggtccctact gcgtgctcgc ggtgaacgcc
     9961 gtggaggcgc tgcgccgcca cggcttcgac gccgtgcgcg ctcccgaggg tgtgcgggag
    10021 tggcgcgagc tcggactcga gatcacagac acaaacggct gaaaggaacc accacgtgaa
    10081 gtacctgttc atcctcaacg accccgccta cggcaccgag cgcagctaca acggcctgcg
    10141 tctggccatg accctgctga cgcaggagcc cgaggccgag gtgaccgtct tcctcatggg
    10201 cgacgcggtc acgtgcgcga aggccggcca gaagaccccg gacggctact acaacctcga
    10261 gcgcatgctc aagccgatcg tgcgcaaggg cgaggtgctg ctctgcggca cctgcatgga
    10321 tgcccgcgcg atgaccgaga ccgagatctg tgagggtacg aggcgcagtt ccatgagcga
    10381 gctcaccgcg aagacggccg acgccgacaa ggtgctcgtc ttctgacgcg atgatctacc
    10441 tcgaccacaa cgccagcacg ccgatcgccc cacaggtgcg ggaggccatg ctgcccttcc
    10501 tcgccgagca ccacggcaac ccgtcgagcg ctcactgggc gggagcaccg gcggcgcagg
    10561 ccgtcgccga cgcgcgcgag cagctcgccg ccctcctcgg ctgctcgccg cacgaggtcg
    10621 tcttcacgag cggcggcacg gaggcgaaca acacggcgct caagggcgtc gtcgaggcag
    10681 ccggcgccga gcggccgcac atcgtgacca cggcggtcga gcatcccgcg atcgtcgagc
    10741 cctgccgcta tctggagcgg cacggagtgg acgtgaccgt cgtcggcgtc gacgccactg
    10801 gcctggtcga ccccgacgac atcgccgccg cgatccggcc ggagaccgtg ctcgtctccg
    10861 tgatgcacgc caacaacgag gtcggcaccg tcgagccgat cgccgagatc tcgcgcatca
    10921 cgcgggaggc gggcgtgctc ctgcacagcg acgcggcgca gtcggtgggc aaggtcccca
    10981 cccgcgtcga cgacctcggc gtcgacctgc tctccgtcgc cgcgcacaag ttctacgggc
    11041 ccaagggcgt gggcgccctc tacgtgcgcg aggggacgag actcgagccg ttcatgcacg
    11101 gcgccggcca tgaggacggg cgccgcgccg gcaccgagaa cgtgctcctc gacgtggccc
    11161 tgggggccgc cgccgagctt gccggcgacc tggcgtggac gctcggcgtg cgcgacctgc
    11221 gcgactggct ctggctccag ctgcaggacc gatggggcga gcgcgtggtc ctgaacgggc
    11281 acccgacggc gcgcctgccc aacaccctga acgtgagctt cctcggccgc gacggccggg
    11341 cggtcctcgc ggccatgccc gaggtcgccg catccaccgg ctccgcctgc cacagcggcg
    11401 gcaccgagcc gtcgcccgtc ctggcggcga tgggcgtacc ccgtgaggtc ggcgccggcg
    11461 cgatccggct cagcctgggg cgcaagacca cccgcgcgga gctcgagacg ctgctggagc
    11521 gattggccga agtcctctcc gacggcggcg aacggtgagc gctacccgga gactggaccg
    11581 gctgcgcttc ggcaccttcc tcgcgcccaa catcatgccg gtgtacgagg ccgtgaccga
    11641 agaggtcggg cgccgcctcg gggtcgagac cgagctgttc atcgagaccg actacgaggc
    11701 gtgcgcgaag gacgacaacg aggtctgctt cgtctgcagc ctcccctacg tcgactacga
    11761 gcgtcgcggg ctctctcccg ccgtcccggt cgccgcaccg gtgctcgtgg gagaccgcta
    11821 cgccggcaag ccggtctact tctcggatgt gatcgtccac cgggacagcc ccttccggtc
    11881 gttcctcgac ctgcgcggcc actcctgggc ctacaacgag ccgctctcgc actcgggcta
    11941 cgggatcacg cgctaccacc tgctcgaggt gggggagacc cacgggttct tcagcgaggt
    12001 catcgaggcc ggcttccacg agacgtcgat gcggctggtc gcggcaggcg aggtcgacgc
    12061 ctccgccatc gactcccagg tgctggccgt ggccctgcgc gacgatccct cgctggcccg
    12121 ctcgttgcgc gtcatcgacg ccctgggtcc ctcgaccatc cagcccgtgg ccgtgtcccg
    12181 gcgcgtgccg gccaggcagc gcgacgagat acgcgaggtc ctgacgacga tgcacgagga
    12241 cccgccgatg cgtgagcgtc tcgccctcgg catggtggag cggttcgtgc cggccgacgc
    12301 ctcgtcctac gacgacatcc gcatgatgct ggaagcctgt gaggcagcag ggttcatgga
    12361 agtcaggtag gcggggagga gcggaagggc actggagccc ggtcgcggat tcgaaccgcg
    12421 gaccccctcc ttaccatgga ggtgctctgc ctgctgagct aaccgggctt ggtgggggag
    12481 ccaggatttg aacctgggta ggctgcgcca acggatttac agtccgcccc ctttaaccac
    12541 tcgggcactc ccccgggagc cgtggaatcg tagccgaaag cggaggcgct ttcaaccggg
    12601 agcgaggcgg tcagggtggg tccggaaaga aggcagccgg ggtcgtcaag gggatgacga
    12661 ccccggcttg aaagggtggt cgcccggcca ggggaagcgg gcgagcacgg gactcgcagt
    12721 gtcatcgcgt cctgcggtgg gagggggatc ccggcccgcg gcgcttgtga tgacatgcgc
    12781 attctaaccg aaatacgggc gcacgcaacc gaagtgcgga caaaatcgtt ccggcggcgt
    12841 ttgtaccgat tgtccaagcg ccggtgccgc tgcggccgcc ggcgtgccag tgcagccact
    12901 gccgaacggc gcaagcggac agattgagcg cctccggagc ctgtggtacc gtaagcatcc
    12961 gccgtggcgg cgtagctcag ctggtcagag cagcggaatc ataatccgcg tgtcgtaggt
    13021 tcgagtccta ccgccgctac cacttctctt cagcagcctc ctcctcgcgg cacaaccccc
    13081 tccccgacgt gtcctgatcg gacaacggcc acctcggcaa acgctactct t
//
LOCUS       DASUMX010000009         9434 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_865640, whole genome shotgun sequence.
ACCESSION   DASUMX010000009 DASUMX010000000
VERSION     DASUMX010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 9434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9434
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_865640"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..965
                     /gene="accB"
                     /locus_tag="VFZ86_00340"
     CDS             <1..965
                     /gene="accB"
                     /locus_tag="VFZ86_00340"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEX5640764.1"
                     /translation="VYSHQVPGGMISNLESQLKEQSALDRLPEVLQEIPIVRAEVGFP
                     PLVTPLSQIVGTQAVLNVLSGRRWHIVPDEMKQYLRGKYGAAPGPVSREVLLRVLEGE
                     EPIGVRPADLVDETLEDYRAEIGELARSEEDLLSYALFPQTARVYLERHHIGPESDVF
                     GVHDHYLSSQAGADLLEQSADRVKDILGMIEESEVDEVVIEEGDLKITVRKAGVEPAV
                     APAAAVQAAAPAAAAGADEGTNGYHLVKSKWVATFYRSPTPQAPSFVEVGDQVEAGQT
                     LCILEMMKMMNELTADVAGVVREILVENGETVQYGQPLFAIEPA"
     gene            988..1428
                     /gene="nusB"
                     /locus_tag="VFZ86_00345"
     CDS             988..1428
                     /gene="nusB"
                     /locus_tag="VFZ86_00345"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEX5640765.1"
                     /translation="MPTAGPSRKQARRDAVFLLYQTEVTDLSMAELVEGLRLREGYAP
                     DEFTIKAVAGVLKTRDELDVVLQAHARDWPLDRIAPLERSILRLAVWEIRTEVTPPEV
                     AIDEAVRLTKRYSTDEAGAFVTGVLGGIVRAELDEAAQEGGGDD"
     gene            1421..1630
                     /locus_tag="VFZ86_00350"
     CDS             1421..1630
                     /locus_tag="VFZ86_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5640766.1"
                     /translation="MTDDAQSAAAPTRAQALARLGEILGQLEELEPRLAASSSAEVEM
                     TLLERATEQVEEAGRLLEDLGRVAG"
     gene            1641..2519
                     /locus_tag="VFZ86_00355"
     CDS             1641..2519
                     /locus_tag="VFZ86_00355"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HEX5640767.1"
                     /translation="MNAGGAAGYPADLLALVEDYLAALDFGADRRAARLTEAMRYSLL
                     AGGKRIRPVLTLATARGRGADPRALLPTAAAFELIHTYSLIHDDLPAIDDDTLRRGRP
                     TCHVAFGEDVAILAGDGLFAEAFDLVLRRQTGGCAAVLAALAEIAAATGVQGMVGGQY
                     MDVAGDAADDDDLRTLHALKTGRLIEAAVVCGALLSGAEDVTPYRAYAAELGLLFQIV
                     DDILDETGAEDELGKTLGKDRALAKVTYVSRFGMDRAVELAGAAERRAVGLLAALPGE
                     TSDLAAVTRFIHDRRR"
     gene            2611..4488
                     /gene="dxs"
                     /locus_tag="VFZ86_00360"
     CDS             2611..4488
                     /gene="dxs"
                     /locus_tag="VFZ86_00360"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012281638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEX5640768.1"
                     /translation="MRYLDTISDPADLRQLSERELTLLADEVRETILSCVSETGGHLA
                     ASLGTVELTVALHSVLESPRDKIVWDVGHQCYAHKLLTGRREGFGTIRQHGGLSGFPC
                     RTESPHDVAGTGHASTSISYGLGLVEAGRIAGGDGGNVVCVLGDGALTGGVAFEALNQ
                     AGHLHTPLVVVINDNEMSIRANVGALQLYLNRIRLDPTLTRLREDLEQGVAKIPAIGR
                     QAYRLGKDVKESMKAFLVPGMLFEELGFAYIGVVDGHDMHALRESIRQAIDTRRPVVV
                     HVKTVKGKGYEPAEARPDTYHGTGPFHIGNGARKAAAVGATYTDAFGEALVRLAERDE
                     RIVAITAAMTQGTGLEAFERRFPDRFYDVGIAEEHAAVFAAGLAIGGMRPVVALYSTF
                     LQRAFDMLVQDIGLQDLPVTFAVDRAGLVGDDGPTHHGAFDLSFLRVIPGMTLMAPSS
                     QEELQRMLATALTLDGPAALRYPRGLAAPLTALEEITPLEVGRALVLQEGADVALVGV
                     GTGVAIAREAAELLRVEGVTPTVVDARFVKPLDTGLLERLAAGHRRLVTVEENALPGG
                     FGSAVLEAVGSAVEVVRFGLPDAFVPHGDRARILADIGLTPQAVALAARAHSPALTRV
                     K"
     gene            4496..5293
                     /locus_tag="VFZ86_00365"
     CDS             4496..5293
                     /locus_tag="VFZ86_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013941204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEX5640769.1"
                     /translation="MRERLDVLLTDRGLFSSRAQARAAVLAGEITVDGRIVDKPGTPV
                     DDGATIAAAARRRYVSRGGDKLDTALTRLDVDVTGEDAIDLGSSTGGFVDRLLQGGAA
                     RVIAVDVGYGQLDWRLREDPRVTVLERTNARRLTRERLPYEPSFVTADLSFISLTVAL
                     GPVLECLREGFRGLVLVKPQFEAGRERVGKGGVVRDAAVHRDVLRRVAGWLQERGATV
                     QGVCDSGHPGPKGNVEYFILFSDARAAGAAPPPADGLVDDAVEAVHG"
     gene            5286..6155
                     /locus_tag="VFZ86_00370"
     CDS             5286..6155
                     /locus_tag="VFZ86_00370"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HEX5640770.1"
                     /translation="MGEPLQRVAVLTHLFTAATAAALAELAAAADRLGLELLMPADEV
                     RKHPTATGLGYRTVDEAELRHADLCLVLGGDGTILRSLSRLLGTRVPTLGVNYGNVGF
                     LASLPREGWMAGLETILGGDYREVELLTVDVLIGGEHHSAVNDVILSRVAPRHVLQLE
                     YEVAGVTVGEMFCDGLIIASPTGSTAYNLSCNGPIVEWNAGVLVLNFIAPHSLGFRPV
                     ILRPDHVITARNVSPRQECEVMADGDLVGRLCCGDEVRVTAGSEVARLVMQREGSFYQ
                     NVEEKLFDRHDAR"
     gene            6145..7794
                     /gene="recN"
                     /locus_tag="VFZ86_00375"
     CDS             6145..7794
                     /gene="recN"
                     /locus_tag="VFZ86_00375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEX5640771.1"
                     /translation="MLAELSIDDLVLIAAARLEFSPGLNVITGETGAGKSLLAQAIGL
                     LMGQKGGDELVRPGAGRALVQALFEAGDETLAVARELPRGGRSRARMDGLLSSAAAVE
                     DALRRRLAFYGQLEHTRLLQLERQLDLLDGAVADEVEPLKTAYVEAYARARALTRELD
                     DLRGAGRDRERELDMLRYQVHEIEAAGVEPGEDERLTVDRERARHAGKLLERTGGALA
                     LLAGESEAAALDGVRVAQRLVDEAALLDESVAPLAERLGALTVELDDLAAALRDYIDA
                     LDVDAAHRDALELRFDKLKGLMRKYGGSADEVLAYLEDARVRLRALEEFAADEESLIA
                     TVTAAQDAAVAAAARLGDARRRLAPEVAARIEEELRGLAMPHATFVIEVSARGGGWDG
                     LGPSGADDVEFVFGANPGVPPRPLRETASGGELSRTMLAIRGLVTLGDDVETLIFDEV
                     DTGIGGVTAAALGVRLRRLAERRQVLCITHLPQVAAFADRQFAIAKQSDPGAGVTETV
                     VRRVDGDERVEELCRMLGAGPGDEAARSHALSLLEKAGAAG"
     gene            8092..>9434
                     /locus_tag="VFZ86_00380"
     CDS             8092..>9434
                     /locus_tag="VFZ86_00380"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017815724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEX5640772.1"
                     /translation="MTKHIFVTGGVVSGLGKGITAASVGRLLKQRGLKVASQKFDPYI
                     NVDPGTMSPFQHGEVFVTEDGAETDLDLGHYERFLDEFVQRDSNVTTGGIYNSVITKE
                     RRGDYLGGTVQVIPHITNEIKSRVHRLAKLTAVDVLITEVGGTVGDIESLPFLEAIRQ
                     FRNDVGRANVLYIHVTLVPMIGVVGELKTKPTQHSVAELREIGIQPDIIVARSEELLS
                     QGIREKIALFCDVDPEAVISAADADDIYRVVLNMHREKLDDLVVKMLGLEVGPPDLAE
                     WEALVERIDACDKPVTIALVGKYVQLHEAYLSVWEALKHSAIHHGRKLDLQWIDSEEL
                     TPKTVAERLCGADGVIVPGGFGQRGIEGKIDAIRYCRENRVPFLGVCLGMQCAVAEFA
                     RDVCDMAGANSTEFDPESPFPVIDLLPEQKNVADMGGTMRLGASLVKVVPGTKAMA"
BASE COUNT         1351 a         3395 c         3401 g         1287 t
ORIGIN      
        1 aggtgtactc ccaccaggtc cccggcggca tgatcagcaa cctcgagagc cagctcaagg
       61 agcagagcgc gctggaccgg ctgcccgagg tcctgcagga gatccccatc gtccgcgccg
      121 aggtcggctt tccgccgctg gtgacgcctc tcagtcagat cgtcggcacg caggcggtgc
      181 tcaacgtcct gtccgggcgc cgctggcaca tcgtgcccga cgagatgaag cagtacctgc
      241 gcggcaagta cggagccgcg ccgggacccg tctcgcgtga ggtgcttctg cgcgtcctcg
      301 agggcgaaga gcccatcggc gtgcggcccg cggacctggt cgacgagacg ctcgaggact
      361 accgcgccga gatcggcgag ctggcgcgct ccgaggagga tctgctcagc tacgcgctct
      421 tcccgcagac cgcccgcgtg tacctcgaac gccaccacat cggccccgag tcggacgtgt
      481 tcggcgtgca cgaccactac ctcagctcgc aggcgggcgc cgacctgctc gagcagagcg
      541 ccgaccgcgt caaggacatc ctcgggatga tcgaggagag cgaggtcgac gaggtcgtga
      601 tcgaggaggg cgacctcaag atcacggtgc gcaaggccgg cgtcgagccc gcggtcgccc
      661 cagcggcggc cgtccaggct gccgcccctg ccgccgccgc gggcgcggac gaagggacga
      721 acggctatca cctggtcaag tccaagtggg tggccacgtt ctaccgctcg cccacgccgc
      781 aggcgccttc gttcgtcgag gtcggcgatc aggtcgaggc cgggcagaca ctctgcatcc
      841 tcgagatgat gaagatgatg aacgagctca ccgcggacgt tgccggcgtg gtccgcgaga
      901 tcctcgtcga gaacggcgag accgtccagt acggacaacc gctcttcgcc atcgaacccg
      961 cctgaggcgg gggagaggcg acgcagcgtg cccacggccg gtccgagtcg caagcaggcg
     1021 cgtcgtgacg ccgtgttcct tctgtatcag accgaggtca cagacctctc catggcggag
     1081 ctcgtcgagg gcctgcggct gcgcgagggc tatgcgccgg acgagttcac catcaaggcc
     1141 gtcgccggcg tgctcaagac gcgggacgag ctcgatgtcg tgctccaggc tcacgcacgg
     1201 gactggccgc tcgaccgcat cgctcccctg gagcgcagca tcctccggct cgccgtttgg
     1261 gagatccgca ccgaggtgac gccgcccgag gtggccatcg acgaggccgt gcggctcacc
     1321 aaacgctact ccaccgacga ggcgggggcg ttcgtcactg gtgtgctcgg tgggatcgtg
     1381 cgcgcggagc tggacgaggc ggcgcaggag gggggaggcg atgactgacg acgcgcagag
     1441 cgccgcggca ccgacgcgcg cgcaggcgct ggcccggctc ggggagatcc tgggccagct
     1501 cgaggagctc gagcctcggc tcgccgcgtc gtcctcggcc gaggtggaga tgacgctcct
     1561 cgagcgcgcc accgagcagg tcgaggaggc gggccggctc ctggaagacc tcggtcgcgt
     1621 cgccgggtga ggacgcgagc gtgaacgccg gcggggcagc cggctatccg gcggatctgc
     1681 tggccctcgt cgaggactac ctggccgcgc tggacttcgg cgccgaccgc cgcgcggcac
     1741 ggctcacgga ggccatgcgc tactcgctgc tcgccggcgg caaacgcatc aggccggtcc
     1801 tcacgctggc cacggcgcgg ggccgcggcg cggacccgcg cgcgctgctg cccaccgccg
     1861 ccgccttcga gctcatccac acgtactcgc tcatccacga cgacctcccg gcgatcgacg
     1921 acgacacgct gcgccgcggc cggcccacct gccacgtcgc cttcggcgag gacgtggcca
     1981 tcctggccgg cgacggtctc ttcgccgagg ccttcgacct ggtgctgcgg cgccagacgg
     2041 gcgggtgcgc cgcggtgctg gccgcgctcg ccgagatcgc cgcggccacc ggtgtgcagg
     2101 gcatggtcgg cggccagtac atggacgtcg ccggcgacgc ggccgacgac gacgacctgc
     2161 gcaccttgca cgcgctcaag acgggccgcc tgatcgaggc cgccgtggtg tgcggagcgc
     2221 tgctcagcgg cgccgaggac gtcacgccgt accgcgccta cgccgccgag ctcgggctgc
     2281 tcttccagat cgtcgacgac atcctcgacg agacgggcgc cgaggacgag ctgggcaaga
     2341 ccctgggcaa ggatcgcgcg ctcgccaagg tcacctacgt ctcgcgcttc ggcatggacc
     2401 gcgcggtgga gctcgccggc gcggcggagc gccgcgccgt ggggctcctc gcggcgctcc
     2461 cgggcgagac ctcggacctt gccgcggtca ccaggttcat ccacgaccgc cggcgctgag
     2521 gccgccgggg cggcgggcgc gcgcgcggcg ccgtcggccc gtggcggctc gaaaggggag
     2581 cccggtgccc ggtgctatac tccggtgacg atgcggtatc tggacacgat ctcagacccc
     2641 gccgacctcc gacaactctc cgagcgtgaa ctcacgctgc tcgccgacga ggtccgcgag
     2701 accatcctct cgtgcgtcag cgagaccggc ggccacctcg ccgccagtct cggcaccgtg
     2761 gagctcacag tcgcgctcca cagcgtgctc gagagtccgc gcgacaagat cgtctgggac
     2821 gtcgggcatc agtgctacgc gcacaagctg ctcaccggcc gccgcgaggg gttcggtacg
     2881 atcaggcagc acggggggct ctcggggttc ccttgccgca ccgagtcgcc gcacgacgtc
     2941 gccggcacgg gccacgcctc gacgtcgatc agctacgggc tggggctcgt cgaggcgggg
     3001 cgcatcgcgg gtggcgacgg cggcaacgtc gtctgcgtcc tgggcgacgg cgcgctcacc
     3061 ggcggcgtcg ccttcgaggc gctcaaccag gccgggcacc tgcacacgcc gctggtcgtc
     3121 gtcatcaacg acaacgagat gtccatccgc gccaacgtcg gcgccctgca gctctatctc
     3181 aaccgcatca ggctcgaccc gaccctgacc cgcctgcgcg aggacctcga gcagggcgtc
     3241 gccaagatcc ccgcgatcgg ccgccaggcg taccggctgg gcaaggacgt caaggagtcg
     3301 atgaaggcgt tcctcgtgcc cggcatgctg ttcgaggagc tgggcttcgc gtacatcggc
     3361 gtggtggacg ggcacgacat gcacgcgctg cgcgagagca tccgccaggc gatcgacacg
     3421 cgccggccgg tcgtcgtcca cgtcaagacg gtcaagggca agggctacga gcccgcggag
     3481 gcccggcccg acacctatca cgggaccggc ccgttccaca tcggcaacgg ggcgcgcaag
     3541 gccgcggcgg tcggcgcgac gtacacggac gccttcggcg aggcgctcgt gcgtctggcc
     3601 gaacgcgacg agcgcatcgt ggcgatcacg gcggcgatga cgcagggcac ggggctcgag
     3661 gcgttcgagc ggcggttccc cgaccgcttc tacgacgtcg gcatcgcgga ggagcacgcc
     3721 gccgtgttcg ccgccggcct tgccatcggc ggcatgcggc cggtcgtggc gctgtactcg
     3781 acgttcctgc agcgggcgtt cgacatgctc gtgcaggaca tcgggctgca ggatctgccg
     3841 gtgaccttcg ccgtcgacag ggcgggcctc gtgggcgacg acgggcccac gcaccacggc
     3901 gccttcgatc tctcgttcct gcgcgtcatc ccgggcatga ccctgatggc cccgtccagc
     3961 caggaggagc tccagcgcat gctggccacg gcgctcacgc tggacggacc ggcggcgctc
     4021 cgctacccgc gcggcctcgc ggcgcccttg acggcgctcg aggagatcac gccgctcgag
     4081 gtggggcggg cgctggtgct ccaggagggc gccgacgtgg cgctcgtcgg cgtgggcacc
     4141 ggcgtcgcca tcgctcgcga ggcggccgaa ctgctgcgcg ttgagggtgt cacacccacg
     4201 gtggtcgacg cccgcttcgt gaagcccctg gacacgggac tgctggagag gctggccgcc
     4261 ggccaccggc ggctcgtcac cgtcgaggag aacgcgctgc ccgggggctt cggcagcgcg
     4321 gtgctcgagg cggtcgggag cgcggtggag gtcgtgcgct tcgggctgcc ggacgcgttc
     4381 gtcccgcacg gggaccgcgc gcgcatcctc gccgacatcg gcctcacgcc gcaggcggtc
     4441 gcgctcgcgg cgcgagcgca cagcccggcg ctgacgcgcg tcaagtaggc caccgatgcg
     4501 cgagcggctg gacgtgctgc tcacggaccg cggcctcttc tcctcccgcg cccaggcgcg
     4561 agccgccgtc ctcgcggggg agatcaccgt ggacggccgc atcgtcgaca agcccggcac
     4621 gccggtcgac gacggcgcca cgatcgccgc cgccgcacgc cgccgctacg tctcgcgcgg
     4681 cggcgacaag ctcgacacgg cgctcacgcg cctggacgtc gacgtgaccg gcgaggacgc
     4741 gatcgacctg ggcagctcga ccggtggctt cgtcgaccgc ctgctgcagg gcggcgccgc
     4801 gcgcgtgatc gccgtcgacg tcggctacgg gcagctggac tggaggctgc gcgaggaccc
     4861 gcgcgtcacc gtgctcgagc gcaccaacgc ccggcggctg acccgcgaac gtctcccgta
     4921 cgagccctcg ttcgtcaccg ccgacctgtc gttcatctcc ctgacggtgg ctctggggcc
     4981 ggtgctagaa tgcctgcgcg agggcttccg cggcctcgtg ctggtcaagc cgcagttcga
     5041 ggccggccgg gagcgcgtcg gcaagggcgg ggtggtacgg gatgccgccg tccaccgcga
     5101 cgtgctccgg cgcgtggccg gctggctgca ggaaaggggc gccacggtgc agggcgtgtg
     5161 cgactcggga cacccgggac cgaagggcaa cgtggagtac ttcatcctct tcagcgacgc
     5221 tcgtgccgcc ggggcggctc cgccgccggc ggacggcctt gtcgacgacg cggtggaggc
     5281 ggtccatggg tgagcccctg cagcgcgtcg cggtgctcac gcacctgttc accgcggcta
     5341 cggcggccgc gctggcggag ctggccgcgg ccgccgaccg gctcggtctc gagctgctca
     5401 tgccggccga cgaggtgcgc aagcatccca cggccaccgg cctcggctac cgtaccgtgg
     5461 acgaggccga gttgcgccat gccgacctgt gcctcgtctt gggcggcgac ggcaccatcc
     5521 tccgtagcct gtcccgcctg ctcgggacgc gggtgccgac gctgggcgtc aactacggca
     5581 acgtcggctt cctcgcctca ctgccgcgcg agggctggat ggcgggtctc gagaccatcc
     5641 tcggcggcga ctaccgcgag gtggagctgc tcaccgtcga cgtcctcatc ggaggtgagc
     5701 accacagcgc cgtcaacgac gtcatcctca gccgcgtcgc cccgcgccac gtgctgcagc
     5761 tcgaatacga ggtcgccggc gtcaccgtcg gcgagatgtt ctgcgacggg ctcatcatcg
     5821 cgtcgcccac cggctcgacg gcgtacaacc tctcctgcaa cgggcccatc gtcgagtgga
     5881 acgccggcgt gctcgtgctc aacttcatcg ccccgcattc gctggggttc cgtccggtga
     5941 tcctgcgccc ggaccacgtg atcacggcgc gcaacgtgtc gcctcggcag gagtgcgagg
     6001 tgatggccga cggagacctc gtcgggcggc tgtgctgcgg cgacgaggtg cgcgtcacgg
     6061 cggggtcgga agtcgcgcgt ctcgtcatgc agagggaggg gtcgttctac cagaacgtcg
     6121 aggagaagct cttcgaccgc cacgatgctc gctgaactga gcatcgacga cctggtcctc
     6181 atcgccgcgg cccggctgga gttctcgccg ggcctcaacg tgatcacggg tgagaccggc
     6241 gcgggcaaga gcctgctggc gcaggcgatc gggctgctca tggggcagaa gggcggcgac
     6301 gagctcgtcc ggccgggcgc cggccgggcc ctcgtgcagg ctctcttcga agccggggac
     6361 gagacgctgg cggtggctcg cgagctgccg cgcggcggcc ggtcgcgagc ccggatggac
     6421 gggctgctga gcagcgccgc cgccgtcgag gacgccctgc ggcggcgcct cgccttctac
     6481 ggacagctgg agcacacgcg cctcctccag ctcgagcgcc agctcgatct gctcgacgga
     6541 gccgtcgccg acgaggtcga gccgctcaag accgcctacg tcgaggccta cgcccgcgcc
     6601 cgcgcgctca cgcgcgagct cgacgacctg cgcggcgccg ggcgcgaccg cgagcgcgag
     6661 ctcgacatgc tgcgctacca ggtgcacgag atcgaggcgg cgggcgtcga gccgggcgag
     6721 gacgagcgcc tgaccgtcga cagagagcgg gcgcgtcacg ccggcaagct gctcgagcgc
     6781 accggcggcg cgctcgccct gctggcgggc gagtccgagg cggccgcgct cgacggcgtc
     6841 cgcgtggcgc agcgcctggt cgacgaggcg gcgctgctcg acgagagcgt cgcgccgctg
     6901 gcggagcggc tcggcgcgct cacggtggag ctcgacgacc tcgcggccgc gctgcgcgac
     6961 tacatcgacg cgctcgatgt ggacgccgcg caccgcgacg cgctcgagct ccgcttcgac
     7021 aagctcaagg ggctcatgcg caagtacggc ggctccgccg acgaggtgct cgcctacctc
     7081 gaggacgccc gcgtccggct gcgcgcgctc gaggagttcg ccgccgacga ggagtcgctc
     7141 atcgcgacgg tgacggcggc gcaggacgcg gcggtcgccg ccgccgcgcg cctcggcgac
     7201 gcccggcggc gcctggcgcc ggaggtcgcc gcacgcatcg aggaggagct gcgcgggctg
     7261 gccatgccgc acgcgacctt cgtcatcgag gtgtccgcgc gcggcggcgg gtgggacggg
     7321 ctcggcccgt cgggcgccga cgacgtggag ttcgtcttcg gcgccaatcc gggcgtgccg
     7381 ccccgcccgc tgcgagagac cgcgtcgggg ggcgaactgt cgcggacgat gctcgccatc
     7441 cgcggactcg tgaccctcgg cgacgacgtc gagacgctca tcttcgacga ggtcgacacg
     7501 ggcatcggcg gcgtgacggc ggcggcgctg ggcgtgcggc tccgccgcct ggccgagcgc
     7561 cgccaggtgc tctgcatcac gcatctgccg caggtcgccg ccttcgccga ccggcagttc
     7621 gcgatcgcca agcagagcga cccgggcgcg ggcgtcacgg agacggtcgt ccgccgggtc
     7681 gacggcgacg agcgcgtgga ggagctctgc cgcatgctcg gcgccggccc cggcgacgag
     7741 gcggcgcgca gccacgccct gagcctcctc gagaaggccg gcgccgccgg ctgatcgacg
     7801 ccggagccgt cccctggggc gtcttccggg gggaggggag cgaccaccgg cgctccttgc
     7861 ggcggccggg cccgatgcta tacttgccgt cgtgatccga gggctcgccg ggcatatcat
     7921 cgtgccggcc cgtgcattca gaacgggcag gaccccggcc ccacaaaatc ccccgctcgt
     7981 cttgtgcagg tccgccgtgc cccgggtgcg ttccctggtg gctgcgaccg gagcgttcga
     8041 cgacggctgc gttcagaacg gcgggatcaa caccaggagg gcacaggggg tatgaccaag
     8101 cacatcttcg tcacgggtgg agtggtcagc gggctcggca aaggcatcac cgcggcgtcc
     8161 gtcggccggt tgctcaagca gcgcgggctc aaggtcgcga gccagaagtt cgacccgtac
     8221 atcaacgtcg acccgggcac catgagcccg ttccagcacg gcgaggtgtt cgtcaccgag
     8281 gacggcgccg agaccgacct ggacctcggc cactacgagc gcttcctcga cgagttcgtc
     8341 cagcgcgact ccaacgtcac cacgggcggc atctacaaca gcgtcatcac caaggagcgc
     8401 cgcggcgact acctgggcgg caccgtgcag gtcatcccgc acatcaccaa cgagatcaag
     8461 agccgcgtgc accggctggc caagctcacc gcggtcgacg tgctcatcac cgaggtcggc
     8521 ggcactgtcg gcgacatcga gagcctcccc tttctggagg ccatccgcca gttccgcaac
     8581 gacgtcggcc gcgccaacgt gctgtacatc cacgtcaccc tcgtgcccat gatcggcgtg
     8641 gtcggcgagc tcaagaccaa gccgacgcag cactccgtcg ccgagctgcg cgagatcggc
     8701 atccagcccg acatcatcgt cgcccgctcc gaggagctgc tctcgcaggg catccgcgag
     8761 aagatcgccc tgttctgcga cgtcgacccg gaggcggtca tctcggcggc ggacgccgac
     8821 gacatctatc gcgtcgtcct gaacatgcac cgcgagaagc tcgacgacct cgtcgtcaag
     8881 atgctcgggc tcgaggtcgg tcccccggac ctcgccgagt gggaagcgct cgtggagcgc
     8941 atcgacgcct gcgacaagcc cgtcaccatc gccttggtcg gcaagtacgt gcagctgcac
     9001 gaggcctacc tctccgtgtg ggaggcgctc aagcactcgg ccatccacca cggccgcaag
     9061 ctcgacctgc agtggatcga ctccgaggag ctcacgccca agacggtcgc cgaacggctc
     9121 tgcggcgcgg acggcgtcat cgtcccgggc gggttcggcc agcgcggcat cgagggcaag
     9181 atcgacgcca tccgctactg ccgcgagaac cgcgtgccgt tcctgggtgt gtgcctgggc
     9241 atgcagtgcg ccgtcgccga gttcgcgcgc gacgtgtgcg acatggccgg ggccaactcc
     9301 accgagttcg accccgagtc gccgttcccg gtgatcgacc tgctccccga gcagaagaac
     9361 gtcgccgaca tgggtgggac gatgcgtctc ggcgcctcgc tcgtgaaggt ggtcccgggg
     9421 acgaaggcca tggc
//
LOCUS       DASUMX010000010         9032 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_2016059, whole genome shotgun sequence.
ACCESSION   DASUMX010000010 DASUMX010000000
VERSION     DASUMX010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 9032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9032
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2016059"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..349)
                     /locus_tag="VFZ86_00385"
     CDS             complement(20..349)
                     /locus_tag="VFZ86_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012866249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEX5640773.1"
                     /translation="MPDTPYLVAASLTMFAVTFALRALPFAALRPLRDSALVGYLSRH
                     MPAGIMVILVVYTLRGISLGEAPHGLPEALAVAATVVLHLVRANAVASIIGGTAVYVI
                     LVNLVFT"
     gene            complement(342..1070)
                     /locus_tag="VFZ86_00390"
     CDS             complement(342..1070)
                     /locus_tag="VFZ86_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012866248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEX5640774.1"
                     /translation="MSTPSSDAAGKPRAQRSALAAAFVDTISVGVGMFPLGAAFGLLV
                     VQSGLDWWWALVLSVLVYAGSMEFLAVGLLVAVTPLPQLALTTLLVNFRHVFYALSFP
                     LQRVRGRAGKAYSMYALTDEAYALTAARPGVEMSSARILWLQGFCQGYWVAGSVAGAL
                     LGSWLAMDLAGLAFTLTALFVVLALDAVRVERDLPTPVLAFVCAAVALAVTPGQMLIT
                     GMGLFVATLVVRFLMRSTGDEADA"
     gene            1112..1561
                     /locus_tag="VFZ86_00395"
     CDS             1112..1561
                     /locus_tag="VFZ86_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009179683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5640775.1"
                     /translation="MKATYRHTSIVAADWRVLASFYEEVFGCTPVPPPRDLSGAWLAR
                     GTGVPDAHLTGIHLRLPGHGEGGPTLEIFGYAATESRPAVAANREGIAHIAFEVDDVA
                     AAARLVLEHGGSRVGQVTAKEVPGVGGLTFVYVADPEGNIVELQAWA"
     gene            1645..2025
                     /locus_tag="VFZ86_00400"
     CDS             1645..2025
                     /locus_tag="VFZ86_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006073476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3465 domain-containing protein"
                     /protein_id="HEX5640776.1"
                     /translation="MSDAGGPSPDAAAARAGDAALARAFDDRVSGLEVEGAGTVERLL
                     ADDEKGSRHQRFILRLATGQTLLVAHNIDVAPRIDDLRVGDVVAFRGVYEWSERGGTI
                     HWTHHDPSGDHAAGWLRHGGRAYQ"
     gene            2176..3180
                     /gene="ilvC"
                     /locus_tag="VFZ86_00405"
     CDS             2176..3180
                     /gene="ilvC"
                     /locus_tag="VFZ86_00405"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEX5640777.1"
                     /translation="MYYDKDADLSLIQGKTVAIIGFGSQGHAHAQNLRDSGVTVVVSE
                     LKGTPNGERAEKAGFEVLTAAEATQQADVIMMLVPDQTQAVVYRNDVAPNLKDGATLM
                     FAHGFNIHFGQIVPPASVDVSMVAPKGPGHLVRRQYEEGKGVPVLIAVYQDASGKARE
                     QAMAYASAIGGLRAGGLETTFEEETETDLFGEQAVLCGGATELVTAGFETLVEAGYQP
                     EIAYFECLHELKLIVDLMWEKGIAGMRYSISDTAEYGDLTRGPRVIDDNVRETMSEIL
                     AEIQSGEFAREWILENQANRPVFNALQRQGKEHLIETVGKELRGMMSWIDEGGLDIDV
                     "
     gene            3222..4796
                     /locus_tag="VFZ86_00410"
     CDS             3222..4796
                     /locus_tag="VFZ86_00410"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5640778.1"
                     /translation="MNRTMRVLAVGDLAPECIARLEEDGLRVDARPEWGEAELAAGAD
                     GYQALVAGPGAAVTAAVLAAARALVVVGAGDGVAAIDVAAATRRGVVVVHAPDAGLVS
                     EAEHALALVLACARDLGGADAALRGGRAGHVPGAGVEVRGKTLGLVGVCASSSLLVER
                     ARALGIHVLACEAQAPGEVATGVVDCVTPEQVLAQADVLVAQTPPGVPLFGAAELAQL
                     KQGARLVSPRASDAVDAEALAAALAGGRLAGAAVAVAPGVAVSPALLAAPRLLLTSPS
                     EAATADARRRAGVSVAEQVAAVLRGRLLHGAVNVPAASGDDADELMPYMGLCDRLGRL
                     LVQLAGEPVDAVEITYGGSVAYYDTHLLTLGVLGGVLAGRAESPVNFVNAEVVAAELG
                     VTAAETRESGIPDFPRLITVAVAGTGGAMSVSGTSLGSEHKPRLVRVFGEDVDLAPAP
                     HMAVLRYVDAPGIGGKVGTLLGEWGVNIGHMSVGRGKLGDEAVMVLTLDESLTREQTD
                     ALVARCGLVFARAVEL"
     gene            complement(4862..5269)
                     /locus_tag="VFZ86_00415"
     CDS             complement(4862..5269)
                     /locus_tag="VFZ86_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018771341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5640779.1"
                     /translation="MGLSISSVMPTIAVDDLDRAISFYSDKLGLTVRRLEGDTDSALV
                     EVGDSERLLLYRSTYKRGETTYASFFVEDLEGTVRELRDRGVTFEDYDFPGLKTVDGI
                     ATLGELKTSWFKDSEGNILAISNEAAEVMRKAA"
     gene            5800..6960
                     /locus_tag="VFZ86_00420"
     CDS             5800..6960
                     /locus_tag="VFZ86_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HEX5640780.1"
                     /translation="MQKRFLITPGPTPVPPEVLSALAEPVIHHRAPRFTEILKQVVAG
                     MKYIYQTENDIMVYAASGTGAMEAAVTNVVNPGDHVLVASIGNFGERWKKLNATWGAE
                     VTAIDYAWGTKAVPADIEKALAADPAIKAVYVQHSETSTGVVNDIEAIGEVVARTDAI
                     LVVDAISGLGASDLQTDAWHVDIALAGSQKALMVPPGLAYAAVSDKAWAVIEQCRTPR
                     FYFDFVAMRKKMTGDSAQTPYTPAVNLMVAQNAAVDLIKEEGLQNVFERHRVLGKAAR
                     EGVKALGLELFAVEDPEANSVTAVKVPEGVDGGKIGKIARDKYGVWLAGGQGAVKGKI
                     FRFGHCGYFGHSDIVVGLSTVEMVLAELGYDVKFGASVGAAEQVFMQSTVLL"
     gene            6975..8576
                     /gene="serA"
                     /locus_tag="VFZ86_00425"
     CDS             6975..8576
                     /gene="serA"
                     /locus_tag="VFZ86_00425"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEX5640781.1"
                     /translation="MTEQKKVLVKEKLAPEGVKYLEDLGFQVDIGTEWDADELLARIP
                     EYHGLIIRSATKVTADVIQAGENLQVVGRAGVGVDNVDVKEATKRGVIVVNAPQSNVL
                     SAAEQTIALLMACARNLAQAHADLKNGEWTKGRWGKGGVEVRGKTLGIIGLGRIGFLV
                     AEDARGLGMDVLAYDPFVPAEKFHELGLERADSPDKIYREADFITVHLPKNAETLGFI
                     GDDEFAKMKDGVRVINVARGGIIDEEAWARAIESGKVVASAVDVYPKEPTTESPLFKY
                     DSVVATPHLGASTVEAQLRAGMIIAEQVAAVLTGQFASNAVNIPLAPGEDADELMPFL
                     GLCEQLGKLIVQIADGPVDSFEIAYGGGIARYDTRILTLGVLQGILADKVDGPVNFVN
                     VQSIAEERGITAKESKQPAAVDFLNLITVTTHDARGELSVSGTTLGPKHRPRLVKVYR
                     QDVDIEPAPHMAFLRYADVPGMIGKIGTKMGDFGINIGQMGVGREVQDAKAVMGLTLD
                     EPISQEQLRELVAACGLADGKRVEL"
     gene            complement(8675..>9032)
                     /locus_tag="VFZ86_00430"
     CDS             complement(8675..>9032)
                     /locus_tag="VFZ86_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5640782.1"
                     /translation="NASWHAEAAGVSSASVVIQVRPRVTLALSHTQSGARLTEIFSGA
                     VAPAHAGKRVVVQRYTADGWRAVASGRLDRRSHYRIGWLMPYRSAVYRLRVLLPAHDD
                     HADGVSQTAFVRVRVG"
BASE COUNT         1395 a         3205 c         3249 g         1183 t
ORIGIN      
        1 gccgccggcg ccacttcagt cacgtgaaca cgaggttgac aaggatcacg tacacggcgg
       61 tgccgccgat gatgctggcc acggcgttgg ccctcacgag gtgcaggacc accgtggccg
      121 cgaccgccag cgcctcgggc agcccgtgcg gcgcctcgcc gagcgagatc ccgcgtagcg
      181 tgtacaccac gaggatgacc atgatgcccg ccggcatgtg gcggctcagg tagccgacca
      241 gtgcggagtc gcgcagcggc cgcagggcgg cgaacggcag ggcgcgcagg gcgaacgtga
      301 ccgcgaacat cgtcagcgac gcggccacga ggtagggcgt gtcaggcatc ggcctcgtcc
      361 cccgtgctcc gcatgaggaa gcggacgacg agcgtcgcca cgaacagccc catgccggtg
      421 atgagcatct gtccgggtgt caccgccagc gcgacggcgg cgcagacgaa tgcgaggacc
      481 ggggtcggca ggtcgcgctc cacgcggacc gcgtccaggg cgagcacgac gaagagcgcc
      541 gtgagggtga aggccaggcc ggcgagatcc atcgccagcc agctccccag gagcgctccg
      601 gcgacgctgc cggccaccca gtagccctgg cagaagccct ggagccagag gatgcgggca
      661 ctgctcatct ccacgccggg gcgcgccgcc gtgagggcgt aggcctcgtc ggtgagcgcg
      721 tacatgctgt aggccttgcc ggcgcggccg cgcacccgct gcagagggaa ggacagggcg
      781 tagaacacgt gccggaagtt gacgaggagg gtggtcaggg cgagctgggg cagcggtgtg
      841 accgcgacca ggaggccgac ggcgaggaac tccatggacc cggcgtagac gagcacggag
      901 aggacgagcg cccaccacca gtcgagaccc gactgcacga cgaggagccc gaaggcggcg
      961 cccagcggga acatgccgac gcccacggag atggtgtcga cgaaggcggc cgcgagggcc
     1021 gaccgctgcg cgcgcggctt gcccgcggcg tcggatgatg gtgtgctcac ggcgcgatga
     1081 taccggcccg gcgacgggag gcagtgacga catgaaggcc acgtacagac acaccagcat
     1141 cgtggccgcg gactggcggg tcctcgcgag cttctacgag gaggtcttcg ggtgcacgcc
     1201 cgtgccgccg ccgcgcgacc tctcgggagc gtggctcgcc cggggcacgg gcgtgccgga
     1261 cgcgcacctc accgggatcc acctgcggct gccggggcac ggcgagggag ggccgacact
     1321 cgagatcttc ggctacgcgg cgacagagtc ccggcccgcc gtagccgcca accgcgaggg
     1381 catcgcccac atcgccttcg aggtggacga cgtcgccgcg gccgcccggc tcgtgctcga
     1441 gcacggaggg agccgcgtgg gccaggtgac cgccaaggag gtcccggggg tcggcggtct
     1501 cacgttcgtg tacgtggccg accccgaagg caacatcgtc gagctgcagg cgtgggcgtg
     1561 aggcctcgcc gcagcgcggc gcggctcgtg gcgctcgtcg ccgcgctcct cgcgctggcg
     1621 ctacccgtca tgggatgcgg ctcgatgagc gacgccggcg ggccgtctcc ggacgccgcc
     1681 gctgcccgag ccggggatgc ggcactggct cgggccttcg acgaccgcgt ctccggcctc
     1741 gaggtcgagg gcgccggcac ggtcgagcgc ctgctcgccg acgacgagaa gggcagccgg
     1801 caccagcgct tcatcctccg gctggcgacg ggacagacac tgctcgtggc gcacaacatc
     1861 gacgtcgccc cgaggatcga cgacctgcgc gtgggagatg tggtcgcctt ccgcggcgtc
     1921 tacgagtgga gtgaacgggg cgggacgatc cactggacgc atcacgaccc gtccggcgat
     1981 cacgccgccg gctggttgcg gcacggcggg cgagcgtacc agtgagtgcg tccgcggtcg
     2041 ccgcgccggg cccgcggtga cgtcacgcca cccggcggcc ggcgcggccc acgttgccgc
     2101 ggcccctgcc agccagtaga gtcttagctt ccgagtacac cccggccctt cgggccgtcc
     2161 agaggaggac accgcatgta ctacgacaag gacgccgacc tctccctgat ccagggcaag
     2221 accgtcgcga tcatcggctt cggcagccag gggcacgccc acgcccagaa cctgcgcgac
     2281 agcggcgtca ccgtggtcgt cagcgagctc aagggcacgc ccaacggcga gcgcgccgag
     2341 aaggctgggt tcgaggtcct caccgcggcc gaggcgacgc agcaggccga cgtgatcatg
     2401 atgctggtcc ccgaccagac gcaggccgtc gtgtaccgca acgacgtcgc gcccaacctc
     2461 aaggacggcg cgacgctcat gttcgcccac ggcttcaaca tccacttcgg ccagatcgtg
     2521 ccgccggcgt cggtcgacgt gagcatggtc gcccccaagg gccccggcca cctcgtccgc
     2581 cgtcagtacg aggagggcaa gggcgtgccg gtgctgatcg ccgtgtacca ggacgccagc
     2641 ggcaaggcgc gcgagcaggc gatggcctac gcgagcgcca tcggcgggtt gcgcgccggc
     2701 ggcctcgaga cgacgttcga ggaagagacc gagaccgacc tgttcggcga gcaggccgtg
     2761 ctctgcggcg gcgccacgga gctcgtcacc gccgggttcg agaccttggt cgaagccggc
     2821 taccagccgg agatcgccta cttcgagtgc ctgcacgagc tcaagctcat cgtcgacctg
     2881 atgtgggaga agggcatcgc cggcatgcgc tactcgatca gcgacacggc ggagtacggc
     2941 gacctcaccc gcggcccgcg cgtcatcgac gacaacgtgc gcgagaccat gtccgagatc
     3001 ctcgccgaga tccagagcgg cgagttcgcg cgcgagtgga tcctcgagaa ccaggccaac
     3061 cggcccgtgt tcaacgccct ccagcgccag ggcaaggagc atctcatcga gaccgtgggc
     3121 aaggagctcc gcggcatgat gagctggatc gacgagggcg gcctggacat cgacgtctga
     3181 gccgcaccgc ggccggccac gagagcgaag gggcgtgtga catgaaccgc accatgcgcg
     3241 tgctggcggt gggcgacctc gcccccgagt gcatcgcccg gctcgaggag gacggcttgc
     3301 gcgtcgacgc ccgcccggag tggggcgagg cggagctcgc ggccggcgcg gacggctacc
     3361 aggcgctcgt cgccgggccg ggcgcggcgg tcacggccgc cgtgctggcg gcggcgcgtg
     3421 ccctcgtcgt ggtcggcgcc ggcgacggcg tggccgccat cgacgtggct gcggctaccc
     3481 gccgcggcgt cgtcgtcgtg cacgccccgg acgccggcct cgtctcggag gccgagcacg
     3541 ccctcgccct ggtgctcgcc tgcgcccgcg acctgggagg cgccgacgcc gccctccgcg
     3601 gcgggcgggc cggccacgtg ccgggcgccg gcgtcgaggt ccgcggcaag acgctcgggc
     3661 tcgtcggcgt ctgcgcgagc tcctcgctgc tggtcgagcg cgcccgtgcg ctgggcatcc
     3721 acgtgctcgc ctgcgaggcg caggccccgg gcgaggtggc caccggcgtg gtcgactgcg
     3781 tcacgccgga gcaggtgctc gcccaggccg acgtgctcgt ggcgcagacg ccaccgggtg
     3841 tgccgctgtt cggcgcggcc gagctggcgc agctcaagca gggcgcgcgg ctcgtcagcc
     3901 cgcgcgcgtc cgacgccgtg gacgccgagg cgctcgccgc ggcgctcgcc ggaggacgtc
     3961 tggccggcgc cgccgtcgcg gtggcgcccg gcgtggcggt ctcgccggca ctgctcgccg
     4021 cgccgcgtct gcttctgacg agcccttccg aggccgccac cgccgacgcg cgccggcgcg
     4081 ccggcgtctc cgtcgcggaa caggtggccg ccgtcctgcg cggccggctc ctgcacggcg
     4141 ccgtcaacgt gccggccgcg tccggcgacg acgccgacga gctcatgccg tacatgggcc
     4201 tctgcgaccg gttgggcagg ctgctggtgc agctcgccgg agagcccgtg gacgccgtcg
     4261 agatcacgta cggcggcagc gtggcctact acgacaccca cctgctcaca ctcggcgtcc
     4321 tcggcggcgt gctcgccggc cgggcggagt cgccggtgaa cttcgtcaac gccgaggtgg
     4381 tcgccgccga gctcggcgtc accgccgccg agacgcgaga gtcgggcatc cccgacttcc
     4441 cgcggctcat cacggtcgcc gtcgccggga ccggcggcgc gatgtcggtg tcgggcacct
     4501 cgctcggctc ggagcacaaa ccgcggctgg tgcgcgtgtt cggcgaggac gtcgacctgg
     4561 cgcccgcgcc gcacatggcg gtcctgcgct acgtggacgc gccgggcatc ggcggcaagg
     4621 tcggcacgct gctgggcgag tggggcgtca acatcggcca catgtcggtg gggcgcggca
     4681 agctgggcga cgaggcggtc atggtcctca cgctcgacga gtcactcacc cgggagcaga
     4741 cggacgcgct cgtcgcccgt tgcggcctcg tgttcgcgcg ggcggtcgag ctgtagaagc
     4801 gagaagaggc gggggcgccg gccggcgctc ccgcctcttc gcgtccggcg ccgcggccgc
     4861 gtcaggcggc cttgcgcatc acctcggcgg cctcgttgga gatcgccagg atgttgccct
     4921 cgctgtcctt gaaccagctg gtcttgagct cgcccaaggt ggcgatgccg tcgacggtct
     4981 tgaggcccgg gaagtcgtag tcctcgaagg tgacccccct gtcgcgcagc tcgcgcaccg
     5041 tgccctcgag gtcctccacg aagaagctcg cgtaggtggt ctcgccgcgc ttgtaggtac
     5101 tgcggtacag gagcagccgc tccgagtcgc ccacctcgac cagggcgctg tcagtgtctc
     5161 cctcgagccg gcgtaccgtc agaccgagct tgtcgctgta gaagctgatg gccctgtcca
     5221 gatcgtctac ggcgatcgtg ggcatgaccg atgagatcga aaggcccatc ttgcgtcact
     5281 ccttgtcgga tggatgggat aggcgacagg ttccccgtcc tgcgccgaac agtccgttcg
     5341 cggcgagcgg gccggggctt gtgcgtctgg acacaaacga cgcagataag gtatgctcca
     5401 cgtcatgcgg agtccctcac atcgcccgtc gtgacggctg gtgcggcgcg gtccacccgg
     5461 accgcgccgc accagccggc tccgctctct cctgacctca ccctgtgtgt cgccgcctgc
     5521 gcgcgccacg tgactgcgtg cgtgccggcg ggcaccgcga ccggggcccc tggcgtccat
     5581 tcgcgacgtc acggccgccg ccgcccgtgc ccgcggcgcc tgcggttcgt ccggctgcag
     5641 ccgcggctca cgatcgcccg atcgcctggt cctgactgag cattcaatgc ttgtgacgga
     5701 ccgcgtcgat tccgtagaat cgcggtttcc gatcctttgt ccaggtggtc cgccggcccc
     5761 tcccggcagc cgaagacatc cccccgaaag gacgacaaga tgcagaagcg cttcctcatc
     5821 acccccggcc ccacgcctgt gccgccggag gtgctctccg ccctcgccga gccggtcatc
     5881 caccaccgcg cgccgcgctt caccgagatc ctcaagcagg tcgtcgcggg gatgaagtac
     5941 atctaccaga ccgagaacga catcatggtg tacgcggcca gcggcaccgg cgccatggag
     6001 gcggccgtca ccaacgtcgt gaaccccggc gaccacgtgc tggtcgcgtc gatcggcaac
     6061 ttcggcgagc gctggaagaa gctcaacgcc acgtggggcg ccgaggtcac cgcgatcgac
     6121 tacgcgtggg gcaccaaggc cgtcccggcc gacatcgaga aggcgctggc cgccgacccc
     6181 gccatcaagg ccgtctacgt acagcacagc gagacgtcca cgggcgtcgt caacgacatc
     6241 gaggcgatcg gcgaggtcgt ggccaggacc gacgcgatcc tggtcgtgga cgcaatcagc
     6301 ggcctcggcg ccagcgacct gcagacggac gcctggcacg tcgacatcgc cctcgccggc
     6361 tcccagaagg cgctcatggt gccgccgggc ctcgcctacg cggccgtgag cgacaaggcc
     6421 tgggccgtga tcgagcagtg ccggacgccg cgcttctact tcgacttcgt cgccatgcgc
     6481 aagaagatga ccggcgactc cgcacagacg ccctacaccc cggccgtgaa cctcatggtc
     6541 gcgcagaacg ccgccgtcga cctgatcaag gaggaggggc tgcagaacgt gttcgagcgg
     6601 caccgcgtgc tcggcaaggc cgcgcgggag ggcgtcaagg cgctcggtct cgaactgttc
     6661 gccgtcgagg atccggaggc gaactccgtc accgccgtca aggtgccgga gggcgtcgac
     6721 ggcggcaaga tcggcaagat cgcccgcgac aagtacggcg tgtggctcgc cggcggccag
     6781 ggcgccgtca agggcaagat cttccgcttc ggccactgcg gctacttcgg ccacagcgac
     6841 atcgtcgtcg gcctcagcac ggtcgagatg gtgctggccg agctcgggta cgacgtcaag
     6901 ttcggcgcct ccgtcggcgc cgcggaacag gtgttcatgc agagcaccgt cctgctctga
     6961 gagggatgag ccacatgacc gaacagaaga aggtcctggt caaggagaag ctcgcccccg
     7021 agggcgtcaa gtacctcgag gacctcggct tccaggtcga catcggcacc gagtgggacg
     7081 ccgacgagct gctcgcgcgc atccccgagt atcacgggct catcatccgc tcggcgacca
     7141 aggtcacggc cgacgtgatc caggccggcg agaacctgca ggtggtcggc cgcgccggcg
     7201 tcggcgtcga caacgtcgac gtgaaggagg cgaccaagcg cggcgtcatc gtcgtcaacg
     7261 cgccgcagag caacgtgctc tccgccgccg agcagacgat cgcgctgctc atggcgtgcg
     7321 cccgcaacct ggcccaggcg cacgccgacc tcaagaacgg cgagtggacc aagggccgct
     7381 ggggcaaggg cggcgtcgag gtacgcggca agaccctcgg catcatcggc ctcggccgca
     7441 tcggcttcct cgtcgccgag gacgcccgcg gtctcggcat ggacgtgctc gcctacgacc
     7501 cgttcgtgcc ggccgagaag ttccacgagc tcgggctgga gcgcgccgac agcccggaca
     7561 agatctaccg cgaggcggac ttcatcaccg tccacctgcc caagaacgcc gagaccctcg
     7621 gattcatcgg cgacgacgag ttcgccaaga tgaaggacgg cgtgcgcgtg atcaacgtcg
     7681 cccgcggcgg gatcatcgac gaggaggcct gggcacgggc gatcgagagc ggcaaggtcg
     7741 tcgcctcggc cgtggacgtg taccccaagg agccgaccac cgagagcccg ctgttcaagt
     7801 acgacagcgt ggtcgcgacg ccgcacctcg gcgccagcac cgtggaggcg cagctgcgcg
     7861 ccgggatgat catcgcggag caggtcgccg cggtgctcac cggccagttc gccagcaacg
     7921 cggtgaacat cccgctggcg cccggcgagg acgccgacga gctcatgccg ttcctgggcc
     7981 tctgcgagca gctcggcaag ctcatcgtgc agatcgccga cggcccggtc gacagcttcg
     8041 agatcgccta cggcggcggc atcgcccgct acgacacgcg catcctcacg ctcggcgtgc
     8101 tgcagggcat cctcgccgac aaggtggacg gcccggtcaa cttcgtcaac gtgcagagca
     8161 tcgccgagga gcgcggcatc accgccaagg agagcaagca gccggcggcc gtggacttcc
     8221 tcaacctgat cacggtgacc acccacgacg cccgcgggga gctctccgtc tccggcacca
     8281 cgctgggccc caagcaccgc ccgcgcctgg tcaaggtcta ccgccaggac gtcgacatcg
     8341 agccggcgcc gcacatggcg ttcctgcggt acgccgacgt gccgggcatg atcggcaaga
     8401 tcggcaccaa gatgggcgat ttcggcatca acatcgggca gatgggcgtg ggccgcgagg
     8461 tccaggacgc caaggccgtg atggggttga cgctcgacga gccgatcagc caggagcagc
     8521 tcagggagct cgtcgcggcc tgcgggctcg cggacggcaa gcgggtcgag ctctagccgc
     8581 tcgagcgaag agtgtgcgaa cgccgacggg gcggcgggcc gggaggcccg ccgccccgtc
     8641 gcatcgaggc agccctggtc gctgcgccgc ggtcctagcc gactctgacg cgcacgaacg
     8701 ccgtctgcga cacgccgtcg gcatggtcgt cgtgcgccgg gagcaggacg cgcagcctgt
     8761 agacggcgct gcggtacggc atcagccagc cgatccggta gtgcgagcgc cggtcgaggc
     8821 gcccggacgc cacggcgcgc cagccgtcgg ccgtgtaccg ctggacgacc acgcgcttgc
     8881 ccgcgtgcgc gggcgcgacg gcgccgctga agatctcggt gaggcgggcg cccgactggg
     8941 tgtgcgagag cgccagcgtc accctcggcc tgacctggat gacgaccgaa gcgctggaga
     9001 cgccggcggc ctcggcgtgc cacgaggcgt tg
//
LOCUS       DASUMX010000011         4107 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1440873, whole genome shotgun sequence.
ACCESSION   DASUMX010000011 DASUMX010000000
VERSION     DASUMX010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4107
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1440873"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VFZ86_00435"
     CDS             <1..252
                     /locus_tag="VFZ86_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5640783.1"
                     /translation="RQSAVEALVYGNERWDGAGYPEGRRGAEIPRVARAFAVCRRWDP
                     ASHNGDSVADLRGRAAHELDPKMVQRFAAMLRAEQAVHN"
     gene            323..3238
                     /locus_tag="VFZ86_00440"
     CDS             323..3238
                     /locus_tag="VFZ86_00440"
                     /inference="COORDINATES: protein motif:HMM:NF015644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HEX5640784.1"
                     /translation="MDNVRPLRPKKKPAGKPRSGSPVRRRVITGIIVALVVLVFAFGS
                     RILGMYVDWLWFGEVGFREVFWTRIWAQVLVGLVAFAAFLVIIWANVELARRLAPDFR
                     ADVDGTLLEPRSPAVRRWVGIGGAVVCVIVAFIAGVAASGSWQRVLLYFNQVAWGEKD
                     AQFGRDISFYVFSSPFWQGVLSLVMAALIAALVLAAIVHLIMGGIELKQKPGRPAADA
                     ETGPSPFARAQQAAPQIPQVDIKLGGRAVAHLSALLAAIFVVVGIGQLFKAWDLLYST
                     AGAIYGAAYTDVVIRLPMTRVTMVIAFILAAVLVWNIWRRHQWWPLTIAVWVVALIVL
                     RGVVPAVYQSLIVNPNQLSKERDYIARNLEATREAFGLNGIEQTPITPRETLTPARLE
                     ANQATLRNIRLWDPRTLVTSYRQLQELRPYYVFLDADVDRYTVNGVYRQTMLSARELN
                     IEGLPVQAQTWVNQHITYTHGFGVAMSAVNQVTPDGSPDFLVQDVPPRSAPGLEIEQP
                     RIYYGERGTDYTLVNTTEAEFDYPASSGDVYTDGYSGSGGVPISPVLNRLAFSVRYGT
                     IKFFTTSALQSESRLIFRNNIRERVQAAAPFLALDPDPYMVIADGRLWWIQDAYTSTS
                     RYPYSTPQGDLNYLRNSVKIVIDAYNGSMAFYVWDEADPLLSAWRGIYPDLFTPRSEI
                     PEALMEHVRYPETLFDIQAETWTTYHVDGPEILYNKGDQWAIPEDVSTTEGGRMDSYY
                     VIMRLPGEDKEEFIQMLPFVPNGRKNMISWLAARSDEPQYGSQLNILFSRSTTVYGPN
                     QVESAINQDPEISAQRTLWGQQGSEVILGNLLVVPIDESLLYVQPLYLQSEQTQLPQL
                     KRVIVFYRAPEREGSDENAIQVVAMRPTLSEALAAAFGETFDSGSASGDTDADNGTTG
                     GTGGDALGAGARELIARANSEFQAAQEALRAGDFAEYGRRIEALEQALTELQRLQQQ"
     gene            3655..>4107
                     /locus_tag="VFZ86_00445"
     CDS             3655..>4107
                     /locus_tag="VFZ86_00445"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX5640785.1"
                     /translation="MSRHFWRAYGRERQSGLTLIELLVTIIIAGIAFVAIVPVVVQAV
                     QAGQGDRARALALSVAQDRVEKIRQLDYDLITAGNLGRTSNTTHWLYSAEFGPTATTA
                     GRDYAVAYAVEELIDDGGKKLYKRVTVAVTWGDPASQPDIDGAQGVTLR"
BASE COUNT          682 a         1435 c         1344 g          646 t
ORIGIN      
        1 cggcagagcg ccgtggaggc gctcgtgtac ggcaacgagc gctgggacgg cgcgggctac
       61 cccgaggggc ggcgcggggc ggagatccct cgcgtggccc gcgccttcgc cgtctgccgg
      121 cggtgggacc cggccagtca caacggagac agcgtcgccg acctgcgcgg ccgtgcggct
      181 cacgaactcg acccgaagat ggtccagcgc ttcgcggcga tgctccgcgc cgagcaggct
      241 gtgcacaact gacgccggcc cggccgcgcc ctctccggcg ccgccccgtt acgaagttcc
      301 tcgaatctgc catacttacg gcatggacaa cgtgcgccct ctccggccca agaagaagcc
      361 tgcaggcaaa ccgcgcagcg gcagcccagt ccgccggcgc gtcatcaccg gcatcatcgt
      421 cgccctcgtc gtgctcgtgt tcgcgttcgg cagccgcatc ctcggtatgt acgtcgactg
      481 gctgtggttc ggagaggtgg ggttccgcga ggtcttctgg acgcgcatct gggcgcaggt
      541 gctggtcggc ctcgtcgcct tcgcggcgtt cttggtcatc atctgggcga acgtggagct
      601 ggcgcggcgg ctggcgccgg acttccgcgc cgacgtggac ggcacgctcc tcgagccgcg
      661 cagccccgcc gtacgcaggt gggtcggcat cggcggggcc gtcgtctgcg tgatcgtcgc
      721 gttcatcgcc ggcgtggccg cgtccggctc ctggcagcgg gtcctcctgt acttcaacca
      781 ggtcgcctgg ggcgagaagg acgcgcagtt cggccgcgac atctctttct acgtcttctc
      841 gagcccgttc tggcagggcg tcctcagcct cgtcatggcc gccctcatcg ccgcgctggt
      901 gctggccgcc atcgtgcatc tgatcatggg cggcatcgag ctcaagcaga agcccggcag
      961 gccggcggca gacgccgaga cgggtccctc gccgttcgcc cgcgcccagc aggccgcccc
     1021 gcagatcccg caggtcgaca tcaagctcgg cggccgcgcg gtggcccacc tctccgccct
     1081 gctcgcggcg atcttcgtcg tcgtcggcat cggccagctg ttcaaggcct gggacctgtt
     1141 gtactccacg gccggcgcca tctacggagc cgcgtacacg gacgtcgtga tccggctgcc
     1201 catgacgcgt gtgacgatgg tgatcgcctt catcctcgcc gccgtgctcg tgtggaacat
     1261 ctggcgacgg caccagtggt ggccactcac catcgccgtc tgggtcgtcg ccctgatcgt
     1321 gctgcgcggc gtcgtcccgg ccgtgtacca gtcgctcatc gtcaacccga accagctgag
     1381 caaggagcgt gactacatcg cccgcaacct cgaggcgaca cgggaggcct tcgggctcaa
     1441 cggcatcgag cagacgccga tcaccccgcg cgagacgctc acgccggcgc gtctggaggc
     1501 gaaccaggcg acgctccgca acatccgcct ctgggacccg aggaccctgg tcaccagcta
     1561 tcggcagctc caggaactgc ggccgtacta cgtcttcctc gacgccgacg tggaccggta
     1621 cacggtcaac ggcgtctatc gccagacgat gctctcggcg cgcgagctca acatcgaggg
     1681 cttgccggta caggcgcaga cgtgggtgaa ccagcacatc acctacacgc acggtttcgg
     1741 cgtggcgatg agcgccgtca accaggtgac tccggacggc tctcccgact tcctcgtcca
     1801 ggacgtgccg ccgcggagcg ccccggggct ggagatcgag cagccgcgca tctactacgg
     1861 cgagcgcggc accgactaca ccctggtcaa cacgacagag gcggagttcg actatcccgc
     1921 ctccagcggc gacgtctaca ccgacggcta ttccggcagc ggcggggtgc cgatctcacc
     1981 cgtcctcaac cgcctggcgt tctccgtccg ctacgggacg atcaagttct tcacgacctc
     2041 ggcactgcag agcgagagcc gcctcatctt ccgcaacaac atccgcgagc gggtgcaagc
     2101 ggcggcgccg ttcctcgcgc tcgaccccga cccgtacatg gtgatcgccg acggtcgcct
     2161 ctggtggatc caggacgcct acacttcgac gagccggtat ccctactcaa cgccgcaggg
     2221 cgatctcaac tacctgcgca actcggtgaa gatcgtgatc gacgcctaca acggttccat
     2281 ggccttctac gtctgggacg aggcagatcc gctgctcagc gcgtggcgcg ggatctatcc
     2341 ggacctgttc accccgcgga gcgagatccc cgaggccttg atggagcacg tgcgctatcc
     2401 agagacgctc ttcgacatcc aggccgagac atggacgacg taccacgtcg acggaccgga
     2461 gatcctctac aacaagggcg atcagtgggc catccccgag gacgtgtcga ccaccgaggg
     2521 cggacgcatg gattcgtact acgtgatcat gcgccttccg ggcgaggaca aggaggagtt
     2581 catccagatg ctccccttcg tgcccaacgg caggaagaac atgatcagct ggctggccgc
     2641 gcgcagcgac gagcctcagt acggcagcca gctcaacatc ctcttctcgc gcagtacgac
     2701 ggtctacggg cccaaccagg tcgagtcggc catcaatcag gacccggaaa tctccgccca
     2761 gcgcaccctg tgggggcagc agggctctga ggtcatcctc ggcaacctgc tcgtggtccc
     2821 catcgacgag tcgctcctgt acgtgcagcc gctctacctg cagagcgagc agacgcagct
     2881 gccgcagctc aagcgcgtga tcgtcttcta ccgcgctccc gaacgtgagg gcagcgacga
     2941 gaacgccatc caggtcgtgg ccatgcggcc gacgctcagc gaggcgctcg ccgcggcctt
     3001 cggcgagacg ttcgactcgg gcagtgcctc gggcgacacc gatgccgaca acggaaccac
     3061 gggcggcacc ggcggcgacg cgcttggcgc cggcgcgcgc gagctcatcg cccgcgccaa
     3121 cagcgagttc caggcggcgc aggaagcgct cagggccggc gacttcgccg agtacggccg
     3181 ccggatcgag gcgctggagc aggccctcac ggaactccag cgcctgcagc agcagtagcg
     3241 gccggggccg gctgcggccg ccggccacca ggcgtagacg aacgttccgt tccctcgcgc
     3301 cccttgtagt ggcagacttg tggcgatgtg cacgagtgcg tctgacgcac acgggcgccc
     3361 gccgcaggcg gcgagtgcag cgagcaagtg tcgtcagcgc gggcaacatg aaggctgcag
     3421 gcgcttgaac gcgccgcccg cggctttcgt caacctgaca gaggtgggaa gatgacggtc
     3481 tggtcgtcgc gacgcacccg caggaggtgg gtgcgcaggt tcgcggccgg ggtgggcgaa
     3541 ccgttaagga cgccgcccgg cggggcaacg gttgagcggc gctgtactca cgtttcatcg
     3601 tcccccgccg atacagcgaa tgaagagact acccgctatc cggaggcagg gtacatgagc
     3661 aggcatttct ggcgggccta tggccgcgag cggcagtcgg gcctcacact catagaactg
     3721 ctcgtcacca tcattattgc cggcatcgct ttcgtcgcaa tcgtaccagt cgtcgttcaa
     3781 gcggttcagg ccggccaagg agatcgcgcc cgagccctcg ctctgagcgt cgcgcaggac
     3841 cgcgtcgaga agatacgaca gctcgactat gacctcatca ccgcgggcaa tctcggccgc
     3901 acatccaaca cgacgcactg gctgtattcg gcggagttcg gacccaccgc aaccactgcc
     3961 gggcgggact acgctgtcgc ctatgcggtc gaagagctga tcgacgacgg tggcaagaag
     4021 ctgtacaagc gggtcacggt tgctgtcacc tggggtgacc cggccagcca gccggacatt
     4081 gacggggctc agggcgtgac gctacgc
//
LOCUS       DASUMX010000012         9428 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1153270, whole genome shotgun sequence.
ACCESSION   DASUMX010000012 DASUMX010000000
VERSION     DASUMX010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 9428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9428
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1153270"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3009
                     /gene="nifJ"
                     /locus_tag="VFZ86_00450"
     CDS             <1..3009
                     /gene="nifJ"
                     /locus_tag="VFZ86_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HEX5640786.1"
                     /translation="ALSPDAPLVRGTSQNPDAFFQGREAGNSFYLDTPGIVAESMAAF
                     ERQTGRGYGLVDYVGAPDAERVVVLMGSGIGAAEQTMETLAAGGEPVGLLKMRLYRPF
                     PAKQFVAALPPTVRAIAVLDRTKEPGALGEPLYLDVLAALAENADSAEPRFERPPRVI
                     GGRYGLSSKEFNPGMAKAVYDELAGADPKRHFTVGITDDVTHLSLAPNTEFRYRRPAG
                     EKQAVFFGTGSDGTVGASKSSVKIIGENTDLYAQGYFVYDSRKAGSVTVSHLRVGPNP
                     IRSSYLIERADFVACHQFGLLGKMDVLGYAREGGTFLLNSPYPADEVWCELPSTVQEL
                     LVRRRLQFWVIDADRIAAEEGMGSRINTVMQPCFFALSGVLPADEAVAHIKESVEKTY
                     GKRGPVIVARNFAAIDRSLQELAQVQVPDAVECGQVAGIAVPDDAPDFVKRVTVPLLA
                     SEGDKLPVSAFPVDGSFITGTTKYEKRGIAREIPIWDPDLCIDCGKCSLVCPHAVIRM
                     KVYEPSHLEGAPEGFRSKDFRAKELPGHKLTIQVSPDDCTGCGVCVDVCPARSNHEVR
                     RKAIGMEPAAEHRAAERENWAFFDAIPTLARDLMPRASVRGSQVLEPLFEFSGACAGC
                     GETPYVKLVSQLFGDRIVVANATGCSSIYGGNLPTTPWTVDRNGRGPAWSNSLFEDAA
                     EFGMGMRLGSKARIRRARLLLERVAPRLDAELVREVLTAPQVREVQIRAQRERVADLV
                     KALEGLGADDPDVRHLLALADDLVGKKVWIMGGDGWAYDIGFGGLDHILASGSNVNIL
                     VLDTGVYSNTGGQASKATPRGAVAKFATSGKSTPKKDLGALAMAYGTAYVAQVAIGAN
                     DTQTVKALLEADAWPGPSLVIAYCTCTAHGIDMSKSMEHMKHAVRSGFWPLYRFQPTP
                     AESGQPFKLDSREPSMPVREFTASEARFAILGRTHPERFERLTELMQNDVDERWRYYE
                     QLAAISRTVASDGVEQADEGPGRSDEEGKR"
     gene            3006..4040
                     /locus_tag="VFZ86_00455"
     CDS             3006..4040
                     /locus_tag="VFZ86_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016329726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HEX5640787.1"
                     /translation="MSRLTTSYLGLELRTPLVASASPWNGDVESALRLQESGASAIVL
                     PSLFEEEVVREELEITRVLEQGTEGFAESLTYFPEVEAFESTADRYLSTIAAMKAALE
                     VPVIASLNATSSGGWVRFARLIEEAGADALELNLYRIAADAARAGAAMEASDLEVVRQ
                     VRASSGIPFAVKLSPYYSALANFAVGVVEAGANGLVLFNRFYQPDLDADSRKVVPRLE
                     LSEPWELRLPLRWLAILRAHTGGAASLAASSGIHHGRDAAKALLVGADVAMMTSAVLR
                     NGREHFATVERELVQWMDEQEYVSVEELRGSVSYAASDDPSAFERANYLKTLRSWSTP
                     VDRTSSPQMP"
     gene            complement(4048..4464)
                     /locus_tag="VFZ86_00460"
     CDS             complement(4048..4464)
                     /locus_tag="VFZ86_00460"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5640788.1"
                     /translation="MSSDDATRDVTAATSAVWDRHVPIPGTPEGFTARTTYPTNPDGV
                     EVMLERWQPGTEEPPHSHPGDDMTVVVEGRMSVQFYRREARRLFPDGERVFLDKGDVG
                     YIRAGRVHDARYIEDCRLVYVHDGAFAFQLADAEAG"
     gene            4749..5030
                     /locus_tag="VFZ86_00465"
     CDS             4749..5030
                     /locus_tag="VFZ86_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018761973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEX5640789.1"
                     /translation="MIGAIILGLIAGAIARFLIPNDALERMGGLVSWVLTLVLGLVGA
                     WLGWWIFTGLLGIGDEDKFDLGGIIGAIVGAVIVLLVATWILRRTGRSA"
     gene            complement(5127..5966)
                     /locus_tag="VFZ86_00470"
     CDS             complement(5127..5966)
                     /locus_tag="VFZ86_00470"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX5640790.1"
                     /translation="MLAIFLALAAGASWGGSCFLSGSQTRRTSLWTVIVFSQLAGLAL
                     MAAIVLARGRVLPEEVLLPALAAGVLSVVSIAASYQALAIGVMCIIGPIISLSVAVPV
                     VVGLAAGERPSALQMAGIAIALGGVVLASREKSAGSCHQSTSRASVGYAVLTAVVWGV
                     VMVLYAKGGETDASWTVLLSRSTSTVILGLGFLVVRPALKLTRAAAAPILAIGVFEVA
                     GNQLFAVAATLGYLSIASVLSSVYPIFVIALAYVFLHERLSRTQQAGVAVALAGVALI
                     AAG"
     gene            6136..7512
                     /locus_tag="VFZ86_00475"
     CDS             6136..7512
                     /locus_tag="VFZ86_00475"
                     /EC_number="4.1.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013067553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-bisphosphate carboxylase"
                     /protein_id="HEX5640791.1"
                     /translation="MDQSSRYADLALDEAELIRGGRHVLCAYRMRPKAGNGYLATAAH
                     FAAESSTGTNVEVSTTDDFTRSVDAIVYEADEAAGVMKIAYPLELFDRNITDGKAMIV
                     SFLTLTIGNNQGMGDVEYAKMHDFYVPPDYLRLFDGPRRNIADFWRVLGRPAEDGGMV
                     VGTIIKPKLGLRPELFADACYRFWLGGDFVKNDEPQGNQVFAPYRETMRLVADALRRA
                     QEETGAAKLFSANITADDPMEMVARGEAVLEAFAGNEDHVAFLVDGYVGGPAAVTTCR
                     RRFPDTFLHYHRAGHGAVTSPQSKRGYTAFVLAKMARLQGASGIHTGTMGFGKMEGDP
                     DDRAIAYMIERDAADGPFYHQEWLGMKATSSMISGGMNALRLPGFFENLGHANVVQTS
                     GGGAFGHLDGPVAGALSLRQAVEAWTAGVDLVVYAGEHRELARAFESFPADADALYPG
                     WRPALGVG"
     gene            7535..8428
                     /locus_tag="VFZ86_00480"
     CDS             7535..8428
                     /locus_tag="VFZ86_00480"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HEX5640792.1"
                     /translation="MSVKHPVIAVTGSSGAGTSTVKTSFEHIFHRENVRPAVVEGDSF
                     HRYGREEMKQAIADAEGKGERTVSHFGPRANLFAELEALFRTYGDSGTGKRRYYVHNE
                     EEAARLGHEPGTFTPWEDLPTDTDVLFYEGLHGGVRAEGVNAARYVDLLIGVVPIVNL
                     EWIQKIHRDTRARGYSVEAATDTILRRMHDYIHYITPQFSHTHVNFQRVPVVDTSNPF
                     VARDIPTLDESLVVVRFRDPTGVDFPYLLAMIHDSFMSRPNCIVVPGGKMEMAIQLVV
                     TPLLQSIMERRKSAKQADGHY"
     gene            8611..>9428
                     /locus_tag="VFZ86_00485"
     CDS             8611..>9428
                     /locus_tag="VFZ86_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5640793.1"
                     /translation="MTALPVAVTVRGYIVASLLVLLVAGAVYLVRRYWPWRAPPAPPE
                     TRPEIDEPPAERHVRSPSDVLRLIVGVLLVLIGLLFAGGASNTLVGFERDLISAFDAL
                     PEVVARAVNLGFQGFASIVFTAILVVALWRRRYRLFVMLVAASLVAQLLLAALDQGIV
                     QQFGEPELLRAIHRPDWVGSRAEIDSNWVASSVAVVILGSPWVSRAWRRAGWITVVLG
                     LVFRAVAGGDVPTDLVLAFGCGVASGAAVLLVFGAPNKRPHGPEAVDAMARAGVP"
BASE COUNT         1475 a         3322 c         3234 g         1397 t
ORIGIN      
        1 gccctcagcc cggatgcgcc gctcgtgcga gggacgtccc agaatcccga cgcgttcttc
       61 cagggacgcg aggccggcaa ctcgttctac ctggacacgc ccggcatcgt cgccgagtcc
      121 atggcggcgt tcgagcgaca gaccggccgc ggctacgggc tggtggacta cgtgggcgcg
      181 cccgatgccg agcgcgtcgt cgtcctcatg ggctccggga tcggcgcggc ggagcagacg
      241 atggagacgc tggccgccgg cggcgaaccc gtgggcctgc tcaagatgcg gctctatcgc
      301 ccgttcccgg cgaagcagtt cgtcgccgcc ctgccgccga cggtgcgggc catcgccgtg
      361 ctcgaccgca ccaaagagcc gggggcgctc ggggagccgc tctatctcga cgtcttggcg
      421 gctctcgccg agaacgccga ctcggcggag ccgcgcttcg agcgcccgcc gcgggtgatc
      481 ggcggccgct acggcctgtc gtcgaaggag ttcaacccgg ggatggccaa ggcggtctac
      541 gatgagctcg cgggggcgga cccgaagcgc cacttcaccg tcggcatcac cgacgacgtc
      601 acgcacctca gcctcgcgcc gaacacggag ttccgctacc ggcgcccggc cggagagaag
      661 caggcggtgt tcttcgggac gggctcggac gggaccgtcg gcgccagcaa gagctccgtc
      721 aagatcatcg gcgagaacac cgacctgtac gcgcaggggt acttcgtcta cgactcccgg
      781 aaggcgggct ccgtcaccgt ctcgcacctg cgcgtgggcc ccaatccgat ccgctccagc
      841 tacctcatcg agcgcgccga cttcgtcgcc tgtcaccagt tcggcctcct cgggaagatg
      901 gacgtgctcg ggtacgcgag ggagggcggg accttcctgc tcaacagccc ctacccggcc
      961 gacgaggtct ggtgcgagct ccccagcacg gtccaggagc tgctcgtgcg caggcgcctg
     1021 cagttctggg tgatcgacgc cgaccggatc gccgccgagg agggcatggg cagccgcatc
     1081 aacacggtga tgcagccctg cttcttcgcc ctgtccggcg tcctgccggc cgacgaggcg
     1141 gtggcgcaca tcaaggagtc ggtcgagaag acctacggga aacgtggccc ggtcatcgtg
     1201 gcgaggaact tcgcggcgat cgaccgctcg ctgcaggaac tcgcccaggt gcaggtgccc
     1261 gacgcggtcg aatgcggcca ggtcgcgggc atcgccgtgc cggacgacgc ccccgacttc
     1321 gtcaagcgcg tgaccgtccc gttgctggcc agcgagggag acaagctgcc cgtcagcgcc
     1381 ttcccggtgg acgggtcctt catcaccggc accaccaagt acgagaagcg tggcatcgcc
     1441 cgggagatcc ccatctggga cccggatctc tgcatcgact gcggcaagtg ctcgctcgtc
     1501 tgcccccacg ccgtcatccg catgaaggtg tacgagccct cgcacctcga gggcgcgccc
     1561 gagggcttcc gctccaagga cttccgggcc aaggagctgc ccggccacaa gctgacgatc
     1621 caggtgtcgc ccgacgactg caccgggtgc ggcgtctgcg tcgacgtctg cccggcgagg
     1681 agcaaccatg aggtgcggcg caaggcgatc ggcatggagc cggcggcgga acaccgtgcc
     1741 gcggagcgcg agaactgggc gttcttcgac gcgatcccga cgctcgcccg ggacctgatg
     1801 ccacgcgcct cggtccgggg ctcgcaggtg ctggagcccc tcttcgagtt ctccggggcc
     1861 tgcgccggat gcggcgagac accctacgtc aagctcgtct cgcagctgtt cggcgaccgg
     1921 atcgtggtcg ccaacgccac cggctgctcg tccatctacg gcggcaatct gccgacgacc
     1981 ccctggaccg tcgaccgcaa cgggcgtggg ccggcgtgga gcaactccct gttcgaggac
     2041 gccgcagagt tcgggatggg catgcgcctg ggctccaaag cacggatccg cagggcaagg
     2101 ctcctcctcg agcgcgtcgc cccacgcctc gacgcggaac tcgtgcggga agtcctgacg
     2161 gcgccgcagg tgagggaggt gcagatccgc gcgcaacgcg aacgagtggc cgatctcgtg
     2221 aaggcccttg aaggcctggg cgccgacgat cccgacgtgc gtcacttgct ggccctggcc
     2281 gacgatctcg tgggcaagaa ggtctggatc atgggcggcg acggctgggc gtacgacatc
     2341 ggcttcgggg gcctcgacca catcctcgcc tccggcagca acgtcaacat cctggtgctc
     2401 gacacggggg tgtactccaa caccggcggg caggcgtcca aggcgacgcc tcgcggggcg
     2461 gtggccaagt tcgcgacctc cggcaagagc acccccaaga aggatctcgg cgcgctggcg
     2521 atggcctacg gcaccgccta tgtcgcgcag gtcgccatcg gcgccaacga cacccagacg
     2581 gtcaaggccc tcctcgaggc cgacgcctgg cccgggccgt cgctcgtcat cgcctactgc
     2641 acctgcacgg ctcacggcat cgacatgtcg aagtcgatgg agcacatgaa gcacgcggtg
     2701 cgcagcggct tctggccgct gtaccggttc cagcccaccc cggccgagag cggccagccc
     2761 ttcaagctgg actcgcgcga gccctcgatg ccggtgcgcg agttcacggc ctcggaggcg
     2821 cggttcgcca tcctgggcag gacgcatccg gagcggttcg aacgcctcac ggagctcatg
     2881 cagaacgatg tggacgaacg ctggcgctat tacgaacaac tcgccgcgat ctcgcggacg
     2941 gtagcttcgg atggcgtcga gcaggcggac gaaggacccg gccgatcgga cgaggaggga
     3001 aaacggtgag ccgtctgacc acgagctacc tcgggctcga gctgcgcacc ccactggtgg
     3061 cgtcggcgag cccctggaac ggggacgtcg aaagcgcgct gcgcctgcag gagagcgggg
     3121 cgtcggccat cgtccttccc tcgctgttcg aggaggaggt cgtccgcgag gaactggaga
     3181 tcacccgggt gctggagcag ggcaccgagg gcttcgcgga gtccctcacc tacttccccg
     3241 aggtcgaggc cttcgagagc accgccgacc gctatctctc gaccatcgcg gcgatgaagg
     3301 cggcgctgga ggtgccggtc atcgccagcc tcaacgcgac ctcgagcgga ggatgggtcc
     3361 gcttcgcgcg gctgatcgaa gaggcggggg ccgacgcgct ggagctcaat ctctaccgca
     3421 tcgcggccga cgccgcccgc gccggagcgg ccatggaggc gagtgacctc gaggtggtcc
     3481 ggcaggtgag ggcgtcgagc ggcatcccgt tcgccgtcaa gctctccccc tactactcgg
     3541 cgctcgccaa cttcgccgtc ggcgtggtcg aggccggcgc gaacgggctg gtcctcttca
     3601 accgtttcta ccagcccgat ctcgacgcgg actcccgcaa ggtcgtgccg agactcgagc
     3661 tgagcgagcc ctgggagctg cggctgccgc tgcgctggct cgcgatcctc cgcgcgcaca
     3721 ccgggggagc cgcgtcactt gccgcgtcga gcggcatcca tcacggccgc gacgcggcca
     3781 aggccctgct cgtcggcgcg gacgtcgcga tgatgacctc ggccgtgctc cgcaacggcc
     3841 gggagcactt cgccacggtg gaacgggagc tcgtgcagtg gatggacgag caggagtacg
     3901 tctccgtgga agagctgcgc ggaagcgtca gctacgcggc cagcgacgac ccgtcggcgt
     3961 tcgagcgggc caactacctg aagacgctgc gctcgtggag cacacccgtg gaccggacgt
     4021 ccagccccca gatgccctga ggcctgctca gccggcctcg gcgtcggcga gctggaacgc
     4081 aaaggcgcca tcgtggacgt acacgagccg gcagtcctcg atgtacctgg cgtcgtggac
     4141 ccggccggcg cggatgtagc cgacgtcgcc cttgtcgagg aagacccgct ctccgtcggg
     4201 gaacaagcgg cgcgcctccc ggcgatagaa ctggacggac atccgtcctt ccacgacgac
     4261 cgtcatgtcg tcgccggggt gtgagtgggg cggttcttcg gtgcccggct gccagcgctc
     4321 gagcatcacc tcgaccccgt cggggttcgt cggataggtg gtgcgcgccg tgaatccctc
     4381 cggcgttccg gggatgggga cgtgcctgtc ccacaccgcg ctggtcgccg cggtgacgtc
     4441 tcgggtcgcg tcatcactgg acatcgtctg gctcctttct cgacctgctc ttctccgcca
     4501 gctcggcgag ccgtccgcta ccattccgcg gagcgccgcc attctacggc agggtggccg
     4561 gtacgcacga tcgttgagac gatgcgcgtc gccggaggtg tgtttcagca cgctggcgcc
     4621 cggacagggt gccaggtgaa gggacccggc ggtcggacgc aaggcgttcc tcgtagccat
     4681 cgccaaggtc gtcgagtacc gcgtcccctg aggggttcgt cgtcgagtcg gctccgggag
     4741 gtatgaacat gataggcgcg atcatccttg gtctcatcgc gggcgccatc gcccgcttcc
     4801 tcatccccaa cgatgcgctc gaacgcatgg gcgggctggt ctcgtgggtg cttaccctcg
     4861 tcctgggcct ggtcggcgcg tggctcggct ggtggatctt caccggcctg ctgggcatcg
     4921 gcgacgagga caagttcgac ctcggcggca tcatcggcgc gatcgtgggc gcggtcatcg
     4981 tgctgctggt cgccacctgg atcctgcggc gtacggggcg ttccgcgtag tctcaccggt
     5041 ggcgctgcag tgaggccctg agaccgcgaa ggcccggccc gaacggcgct tcgacgcagc
     5101 cgttcgggcc ggtccgcgcc gccaggtcag ccggcggcga tcagcgccac acccgcgagc
     5161 gccaccgcca ctcccgcctg ctgcgtgcgc gagaggcgct cgtgcagaaa gacgtaagcc
     5221 agagcgatca cgaagatggg atagaccgag ctcagcaccg aggcgatgct caggtagccg
     5281 agggtcgccg ccacggcgaa cagctgattg ccggccacct cgaacacgcc gatcgcgagg
     5341 atgggagccg cggccgccct cgtgagcttc agtgccggcc gcacgacgag gaaccccaga
     5401 cccaggatga ccgtgctcgt cgaccgggag agaagcacgg tccaggaggc gtccgtctcc
     5461 ccgcccttgg cgtagagcac catcaccacc ccccagacca cggcggtcag caccgcgtag
     5521 ccgacgctcg ctctgctcgt ggactggtgg cacgatccgg cgctcttctc tcgcgacgcc
     5581 aggacgacgc cgccgagcgc gatcgcgatg ccggccattt gcagtgctga ggggcgctcg
     5641 ccggccgcca gaccgacgac caccggcacg gcgacgctga ggctgatgat cggcccgatg
     5701 atgcacatca cgccgatcgc cagcgcctgg tacgacgcgg cgatagagac gacggagagc
     5761 acgccggcgg cgagcgccgg cagcagcact tcctccggaa gcacacggcc tcgagccagc
     5821 acgatcgccg ccatcagggc cagcccggct agctggctga agacgatcac cgtccagagg
     5881 gaggtccgcc gcgtctggct gccggagagg aaacaactgc ctccccacga cgccccggcg
     5941 gcgagggcga gaaagatggc cagcatgccc gcaggctatc agacaggccg gggtcactgc
     6001 ccggcggtga ggcgcgcgag cgccgcggca cgtttgcggc ctgcagcggc acgggcacat
     6061 gaggggaatc ccgcaagcgg ggaggtcgcc ggaggctccg gcccgcacgg cctccggccc
     6121 gaccaggagg cagacgtgga tcaatccagt cgctacgccg atctcgcgct cgacgaagcc
     6181 gagctcatca ggggtgggcg tcacgttctc tgcgcctacc gcatgaggcc aaaggccggc
     6241 aacggctatc tggccactgc ggcccacttc gccgcagagt cctccacggg caccaacgtg
     6301 gaggtctcga ccacggacga cttcacgcgc tcggtggacg ccatcgtcta cgaggcggac
     6361 gaggccgccg gggtcatgaa gatcgcctac ccgctcgagt tgttcgaccg caacatcacc
     6421 gacggcaagg cgatgatcgt ctcctttctc accctgacga tcggcaacaa ccagggcatg
     6481 ggcgacgtcg agtacgccaa gatgcacgac ttctacgtgc cgcccgacta cctgcggctc
     6541 ttcgacgggc cgcgccgcaa catcgccgac ttctggcgcg tcctcggccg gccggccgag
     6601 gacgggggca tggtggtcgg cacgatcatc aagcccaagc tcggtctgcg gccggagctc
     6661 ttcgccgacg cctgctaccg gttctggctg ggcggcgact tcgtcaagaa cgacgagcct
     6721 cagggcaacc aggtcttcgc gccgtaccgc gagaccatgc gcctcgtggc cgacgcgctg
     6781 cgccgcgcgc aggaggagac cggcgcggcg aagctcttct ccgccaacat caccgcggac
     6841 gaccccatgg agatggtcgc ccgcggcgag gccgtgctcg aggcgttcgc cgggaacgag
     6901 gaccacgtcg ccttcctcgt ggacggctac gtcggcggcc cggcggcggt caccacctgt
     6961 cgccgccgct ttccggacac gttcctgcac taccaccgcg ccggccacgg cgccgtgacc
     7021 tcgccgcaat cgaagcgcgg ctacaccgcg ttcgtgctcg ccaagatggc gcgcctgcag
     7081 ggcgcctcgg gcatccacac cggcaccatg ggcttcggca agatggaggg cgatccggac
     7141 gaccgcgcca tcgcctacat gatcgagcgc gacgcggccg acggcccctt ctaccaccag
     7201 gagtggctgg ggatgaaggc cacgagctcg atgatctcgg gcggcatgaa cgccctgcgc
     7261 ctgcccggct tcttcgagaa cctcggccac gccaacgtcg tgcagacctc cggcggcggc
     7321 gccttcggcc acctcgacgg gccggtggcc ggagcgcttt ccctgcgcca ggcggtcgag
     7381 gcgtggaccg cgggcgtgga cctggtcgtc tacgccgggg agcaccgcga gctggcgcgc
     7441 gccttcgagt ccttccccgc cgacgccgac gccctctacc cgggctggcg ccccgccttg
     7501 ggcgtcggct gagcgccggg caaggaggag ccgcatgtcc gtcaagcatc ccgtgatcgc
     7561 ggtgaccggg tccagcgggg ccgggacgag caccgtcaag acgtcgttcg aacacatctt
     7621 ccatcgcgag aacgtccgcc ccgcggtcgt cgagggagac agcttccaca ggtacgggcg
     7681 cgaggagatg aagcaggcca tcgcggatgc cgagggcaag ggcgagcgta ctgtcagcca
     7741 cttcgggcct cgcgccaatc tcttcgcaga gctggaagcg ctgtttcgga cctacggaga
     7801 ctcagggacc ggcaagcgcc gctactacgt gcacaacgag gaggaggcgg ccaggctcgg
     7861 ccacgaaccc ggcaccttca ccccgtggga ggatctgccg accgacaccg acgtgctctt
     7921 ctacgagggc ctccacggcg gcgtgcgcgc ggagggcgtc aacgccgccc ggtacgtcga
     7981 cctgctcatc ggcgtggtgc cgatcgtcaa tctcgagtgg atccagaaga tccaccgcga
     8041 cacacgcgcc agaggctatt cggtcgaggc ggccacggac accatcctgc ggcgcatgca
     8101 cgactacatc cactacatca cgccgcagtt ctcgcacacg cacgtcaact tccagcgggt
     8161 acccgtggtc gacacgtcca accccttcgt cgcccgcgac atccctaccc tggacgagtc
     8221 tctggtcgtc gtccgcttcc gcgatcccac gggcgtcgac ttcccgtacc tgctggccat
     8281 gatccacgac tcgttcatgt cgcgccccaa ctgcatcgtc gtgccgggcg gcaagatgga
     8341 gatggcgatc cagctggtcg tgacgccgct gctccagtcc atcatggagc gtcgcaagag
     8401 cgcgaagcag gccgacgggc actactgagc cggcagccgg cgccgcggca gccacgtcgc
     8461 gacgacgcga cctgagcacg gcggggtgcg aacggattat gtacgcggcc tgggggcacg
     8521 gtctgcgtaa ggcgaagggg ctttccgccc gaggcttggg cttcccgtcg cgagcattga
     8581 cgctcgcgcg cacacgagat gagcgagacc atgacagccc tccccgtcgc cgtgacggtg
     8641 cggggttaca tcgtcgcctc gctgctggtc ctgctggtcg cgggggcggt ctatctggtg
     8701 cgccgttact ggccatggcg agccccgcct gcgccgccgg agacgcggcc ggagatcgat
     8761 gagcctccgg ccgagcgcca cgtacgttcg ccatcagacg tcctgcgcct gatcgtcggc
     8821 gtgctgctcg tcctgatcgg actgctgttc gcagggggcg catcgaacac actcgtcggc
     8881 ttcgagcgcg acctgatcag cgccttcgat gcactgcccg aggtggtcgc gcgggccgtc
     8941 aacctgggct tccagggctt cgcctcgatc gtcttcaccg cgatcctcgt cgtcgcgctg
     9001 tggcgccgcc ggtacaggct gttcgtgatg ctcgtcgcag cgtcgctggt cgcacaactg
     9061 ctgctcgcgg cactcgacca aggtatcgtg caacagttcg gagagcccga gctgctccgg
     9121 gccatccatc gcccggactg ggtgggcagc cgggccgaga tcgactcgaa ctgggtcgcc
     9181 agcagcgtcg ccgtcgtcat cctgggctcg ccctgggtga gtcgcgcgtg gcggcgcgcc
     9241 ggctggatca ccgtggtcct cggcctggtc ttccgcgccg tggccggcgg cgacgtaccg
     9301 accgacctcg tcctggcctt cggctgcggc gtcgcaagcg gcgcggccgt gctcctcgtc
     9361 ttcggggcgc ccaacaagcg cccgcacggg ccggaggccg tcgacgccat ggcgcgagcc
     9421 ggagtgcc
//
LOCUS       DASUMX010000013        34319 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_147287, whole genome shotgun sequence.
ACCESSION   DASUMX010000013 DASUMX010000000
VERSION     DASUMX010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 34319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34319
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147287"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(441..569)
                     /locus_tag="VFZ86_00490"
     CDS             complement(441..569)
                     /locus_tag="VFZ86_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5640794.1"
                     /translation="MTVTASDGGAMRDDHILAERIFLAGIVGCFAILALVIQLIAH"
     gene            complement(679..1938)
                     /locus_tag="VFZ86_00495"
     CDS             complement(679..1938)
                     /locus_tag="VFZ86_00495"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5640795.1"
                     /translation="MLGSRDFRVLLAARLASQVAEGAFLAAVLDTVVFLPESQSTLRG
                     FALATALTLLPFSLVEPFAGVFVDRWARRPILVVLPLVRAAFALLLLPLVGVAALAYA
                     GTLVVFSANRLFQATATAVVPRIVGAGDEATATADARLFAANMVASVAGTVALFGGIF
                     AGGLITSASGTAVTIALACVAWLAASAIGARLSDRLPPREIHDEGVGRQLAAAAADLV
                     DGFRRIGRTPAALAPVLAVAVGQFLQVMVIATTLAVITGVLGGGLLTFSGLVGAGGVG
                     VFLGFLTSGLMRSRMPGPLLMAAAFVLAALALVPVLVTAAPATLIPGAVLLGASYGWT
                     RVPADTLAQRAVPDGYRGRVFTAVDLGFNTARVLGALAAVVVVPLLGPRTTAVFLLVL
                     VLAWAPVVPLWLRTATRAGDAGPPSGG"
     gene            2128..3318
                     /locus_tag="VFZ86_00500"
     CDS             2128..3318
                     /locus_tag="VFZ86_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014531931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent formate dehydrogenase"
                     /protein_id="HEX5640796.1"
                     /translation="MAKILCVLYPDPVDGYPDTYARTDLPVLDRYPNGQTLPTPTGVG
                     FTPGQLLGSVSGELGLREYLEQQGHTLVVTADKDGPDSVFERELADADVVISQPFWPA
                     YLTAERIAGAPNLRLAITAGIGSDHVDLDAAMEHGVTVAEVTYSNSISVAEHVVMMIL
                     ALVRNYIPSYEWVTKGGWNIADCVARSYDLEGMTVGTFGAGRIGLGVLRRLKPFDVDL
                     HYTDKYRLSDDVEQELGLTYHEDVRSLVQACDVLSIHTPLHPETEGLFDRELLATMKR
                     GAYLINTARGKICDRDAVVEALESGQLAGYAGDVWYPQPAPASHPWRTMPHHGMTPHI
                     SGSSLPAQARYASGTREILESFFAGTPIREEYLIVDGGRLAGTGAHTYTAGESASDDS
                     ERAA"
     gene            complement(3402..3818)
                     /locus_tag="VFZ86_00505"
     CDS             complement(3402..3818)
                     /locus_tag="VFZ86_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5640797.1"
                     /translation="MNDTLQRRFARLAGLTLAALLVCAIAAQMTAAYNVAGSGAGGGA
                     VALSGPEPGAAATTSQAAATLQGRGGIASAAADGRASSTPTDSNVWTAILFSLAALLV
                     GAVAWIAIDRRRGRLTGPALAAFCARNPADPLCGAT"
     gene            4036..4530
                     /locus_tag="VFZ86_00510"
     CDS             4036..4530
                     /locus_tag="VFZ86_00510"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5640798.1"
                     /translation="MTGPSDTLEPFRTAHARELAGWAATAAEARRWGGQDVVWPVAAS
                     VVSAWHADPGVRPFVLVRSGMLLGYGEVWIDEEEREVELARIIVRPERRGRGLGRLLV
                     RLLLEQAAQTGHQTAFVRVAPGNAAALACYRNAGLAPVSEEERQAFNEGQPLEYVWLR
                     RELR"
     gene            complement(4590..5048)
                     /locus_tag="VFZ86_00515"
     CDS             complement(4590..5048)
                     /locus_tag="VFZ86_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5640799.1"
                     /translation="MKSSSRRRTVRVAALLVLSLLVLALGAQAVAAASDTSGSASATP
                     APGTEGRGGVAAMSVATSPLAGTAGRGGAAGGGAATIPAPTAGVAAEAQPASSGSSGT
                     PTWIWVGAAVAVLAGSIAAWAVFRRRRSAAEPSAAYCAQHPDDALCRASA"
     gene            complement(5192..5923)
                     /locus_tag="VFZ86_00520"
     CDS             complement(5192..5923)
                     /locus_tag="VFZ86_00520"
                     /inference="COORDINATES: protein motif:HMM:NF014906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5640800.1"
                     /translation="MNDRSPSGLEKLWMREQLESKPRLGLSLERIVESGIALADGDGL
                     AAVSMKRVAESLGFTTMSLYRHVASKDELLLLMHDSAWRPPDGLDIPHGGWRDDLAWW
                     TREQYAIMERHPWLDEVRFIERAGTPSQIAWMDLGLRALGDVQLSEYHKVAVLLLLSG
                     YVSSTARLALTSREGAQRGLFAPDEATAAFGELLRTIVDPGRYPALFRAVEGGAFAPV
                     EGELYAPLDFGLQLMLDGIERLIGA"
     gene            6034..6957
                     /locus_tag="VFZ86_00525"
     CDS             6034..6957
                     /locus_tag="VFZ86_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013001890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5640801.1"
                     /translation="MTAAISVQGLDKSFGSKPALDGLDLTVAAGEVHGFLGPNGAGKT
                     TTLRILLGLLRKDSGAATVLGMDPWRDAAALHRRLAFVPGDVSLWPNLTGGEVIDLLG
                     ALRGGLDRSRRADLIERFQLDPTKKCRAYSKGNRQKVALVAAFASAVDLYLLDEPTSG
                     LDPLMMAVFQDVVREAHAGGGTILLSSHILSEVEALCDHLTIIRAGRTADTGAFEDLR
                     HLTRTTVVVETAEPIDSLAWLPGVHDATIERNRAEFSVGHADLNGVLEHLVGLGITTL
                     TTSPPTLEELFLRHYADTSVTQLEADLERVA"
     gene            6954..8564
                     /locus_tag="VFZ86_00530"
     CDS             6954..8564
                     /locus_tag="VFZ86_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC antibiotics transporter"
                     /protein_id="HEX5640802.1"
                     /translation="MNSFTGTTRLIRLALRRDRFVLAAWLLVLAGLLAMFTAMFVDSL
                     PAQADVLRETRLMAGNAGFRMLSLASGASVGAYAMSRTWVTFALLAAVMSVLCVVRHT
                     RQNEETGRAELLGAGVVGPAAGLTAAVVVALGANVALAPLLGLAMIVGGQPVAGSLAA
                     GASIAAVGAAFTGVAALTSQLSRSARGATGLATAALAVAFLVSGVGNMLGGVDAGGTV
                     AFSAWPSWLSPIGWGYEMRPFGGDHWWLLGLFAAFTGVLLATAGRLAVRRDLGAGALP
                     ERTGRAEASAALRGPFGLAWRLQRPAFLGWLVALAGFGLIFGSVSKSARTMGGSAREM
                     YERTLGTGQMLDAWFTMLIVMGGIAVSIYVVQALLRMREEEARGRLEPILSSAVGRTR
                     WVLSFVLTTALGGTVLLLVFATAVALTAGLALGDSSGLLLDLNAAALAELPAVLVIAG
                     LVVALFAVLPRRAVPVAWVVLGVAVLLSPLFDLDVSQWLADASPFAHQKAPAQEIGMV
                     AVVALLGAAATLVAAGLAWFRRRDLSAG"
     gene            8738..9223
                     /locus_tag="VFZ86_00535"
     CDS             8738..9223
                     /locus_tag="VFZ86_00535"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5640803.1"
                     /translation="MRAWSRVPGEAEGTNVECRQAQRSSDPQRRAPHLETAYPPQRAS
                     AARMRKTLRAYLAQQDVADGIVYQVVLAADEAFTNAVTHAGSDDVIRVSACVSEDAAL
                     VEVHDAGGGFSLAASDPEAVPDLRRPSGRGVFLIEHLMDEVSVESGRCGTTVRMVRRL
                     A"
     gene            complement(9265..10020)
                     /locus_tag="VFZ86_00540"
     CDS             complement(9265..10020)
                     /locus_tag="VFZ86_00540"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HEX5640804.1"
                     /translation="MSAPDPQAQGFDGTGELEAGGDTTEPVKRAILLRETSRTEAFSD
                     GVFAIAITLLALNLAQPVAEKYHDPVALREALLSQWPTYIAFLVSFINILIMWASHHN
                     VFNVIERVDHYFLLINGMVLMAATLTPFSTAILAAHLGTDSAGLAAGVYSIVALYGAL
                     AFNLLWWWAKRRDLIEPLVPPSAIKSVTRRFAIAPAVFTAALVLSFVNSWASVAMFLV
                     VIAHFAVPTVRKHTLDEAEDEIIEQVEEDGPPG"
     gene            complement(10060..11289)
                     /locus_tag="VFZ86_00545"
     CDS             complement(10060..11289)
                     /locus_tag="VFZ86_00545"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5640805.1"
                     /translation="MSLFAESEIVIIGGGAIGCGVAYSLAEAGKRDILLVEREGDVGQ
                     VTTSQGAGLCGQVRSSVERVRLAMHSVDTFRELQRDPEMKPDWHEVGSIRLAFSPSRV
                     QELRRLQAVCEQAGLEVALIGRAEAERLWPPLDLSAVQAVLWCPSDGYMTPLAVAKAY
                     EHRCRRMGVRFETGAAATGITCSDGRVAAVQTDRGEVRCQMAIDAAGAHAYHVARLAG
                     LELPIVPVRHEYYDTVPLRGLHPGLPCFRIPEMTLYGRATGERLRLGGWEAHALSADP
                     RSYRLEEDAPVVEADQRVLDGFEEQYARLLPEARGASRTRVGKGWPTFTPDGRFIIGE
                     SRRLKGFVMAGGCNAHGISGSAGIGRLLVESLFDPHPSDYVRSLSPDRFTERLWNWDT
                     ARRRAQHVYETYYALGR"
     gene            complement(11393..11770)
                     /locus_tag="VFZ86_00550"
     CDS             complement(11393..11770)
                     /locus_tag="VFZ86_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5640806.1"
                     /translation="MRKCIFCGRGVDVVLWVLDDVVDHPVAACTACAEEHGLSVVQSE
                     HDAGDLPEYDSCQCCGRELPSRGGWPLTVWVQDRRRLIAVCNACRAEYALRAVFTSAP
                     PSGKEAHREVEPPGDEPRASSPD"
     gene            complement(11905..12831)
                     /locus_tag="VFZ86_00555"
     CDS             complement(11905..12831)
                     /locus_tag="VFZ86_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5640807.1"
                     /translation="MLITIAGWLVCFAAGVALQRRLRNPQRASYLLFVLVLWVLAPLA
                     VVYGYTTVAVRPELAAAFACVVAAAWATLLAGLLWGRLGGRSQDEAGVLAYATAMGNT
                     AILGYPLATLVFGGPGLALAVVFTEFQFLLPSQGVILGLGRHYAGPDSLWSRAPGMRQ
                     VVRSWLLNPPVVAGVGAVALRLLGVDITTLVEPVGPVVGMLFGFVGFLQVGLAVSLEP
                     LRHDRGDLWRTAVTVALRSGLMPLVLLGLAWLAGVRIPGVFLLLAAMPVAFYTMVVAA
                     VFDLDRGLARLLVAVSTPAVIAGVLLWRLAAG"
     gene            complement(12966..14090)
                     /locus_tag="VFZ86_00560"
     CDS             complement(12966..14090)
                     /locus_tag="VFZ86_00560"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEX5640808.1"
                     /translation="MLVPPSSLPLLAIQGMQLALPIFQGGMGVGVSLAPLASAVAVAG
                     GLGLISSAGLDRIVSRRLGRKVGIYEAVRLEVEAAKARGGFAGVNIMVALQRDFASSV
                     RAALDAGADAIVSGAGLPLSLPSIQPARSTALIPIVSSARALAVICRRWERQHVRPDA
                     VVLEGPLAGGHLGFRADQVDREENTLERLLPPVKEVAAAHGDLPVIVAGGIYSHADIR
                     RFLALGADGVQMGTRFLATEESSASPAYKQAVLRARSEDVVVATAPGSPCGLPFRVLR
                     ESPMYQEALVRGRPARCAKGYVLLKDAKGRFTQCAAKYDNEHFFCICEGLFSAAGYDP
                     HREKPLYTAGATAARVDRILTTGGLMAELAGSPALLPMVA"
     gene            14166..14663
                     /locus_tag="VFZ86_00565"
     CDS             14166..14663
                     /locus_tag="VFZ86_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017364430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX5640809.1"
                     /translation="MPRDPWIAVIADTHGLLPQRVLDACAGADHVIHAGDVGSRDVLD
                     RLTQLAPVSVVRGNRDRDAFGLQLPGEALGEVAGLRFAVVHKRRDLKAAHPDPRAEGL
                     GLLVYGDSHRAEIRYRGHEPGGIPVLWLNPGTASAPLPHDPRPSMARVIVRDGLPEAR
                     IVFFD"
     gene            14971..18549
                     /locus_tag="VFZ86_00570"
     CDS             14971..18549
                     /locus_tag="VFZ86_00570"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HEX5640810.1"
                     /translation="MTRVSKITFLILVALVVVFGFAATALADGRVIQNDPDNPIKFSI
                     DGAIASGAVSTPDLYIGTVGSYLGAEADGSTPVFKEGFGKSPYDILIMNDKVGIVLAV
                     GTPDPWGYPGGSILDAGRVESLPAGATDLKGATFGPDTVLTVQFLFNTWDAWAPANTG
                     IVYCDLVKYDFAAKAIDNVDGVPAVQVKRKFAVPYNDGGVSVARDLDVISYYSIPADE
                     EYAYMFDSVVNNGVAFSAESANEVSLSNKGGDGIDTKTVAALAAANTYNWVADEAGEP
                     TRAFSTTLISPGDNPGSDGRLHPLASFTGARGYRELAFADPGYIVGESRTYESYLMID
                     DECSWQGVIDFYAAYKDLDTFRVAGDVTDQAGAAVPYPVIVIYRGTTLYGWVMGDEAG
                     HYTADLPDEAGAEPYHLQVEKSGTTAGPVSADFTAATVPAEGIDLQAGDYLVPVKFHF
                     RDQDDAPVWGRVSVGTTPTIAFTGQNFFFSDNATDGSVNKGEVTALVKPGDYSATCYG
                     EGFGFYSYTTATNTYSQVVTGNTATDTERTVTITKTLSAPVDWFGIDNHHHGVRSDAF
                     SPPEVVAKAQTTAGLEVLTLDDHEYVIDNIHVYNWARKMDAAGYMPSEEVTPSWAHFD
                     VMPLTEAAYARFLDRDQKNGIVNTNASLQGIMDDGHNAGVAIGANHPNSSYGLFLADD
                     NQTVPGGMTDDFDGIEAQFRVDTLGEAMSYWNAYITGGSHRGVAVKRPHYIYASTDIH
                     DSGGRTTTGARRSYVFVKDGAEQSASDFEAFSLKFARSQAAGHSYSSSGVFVTPTSGK
                     MYGDTYRADATGSFTATFKVSALNPITNIYVFGSTGTGTGTAPFAMTNLVSQTTYAGD
                     DLSTSKDFTLTVDDVSGKQWYAIAAVSSDGKQAFTNPIWVNGTDVTTPVTITDLDPII
                     TTPDAPVAGNVIEQPPTAILTTTPWCGFRLADWELINGDFGSVAQKGVEYVFTLTFYA
                     PKGYVFDPALSTDGNFGRKVSEDGARLIYRAIYTTEPPTTTVSGCPAGWVRGPVTLDF
                     AAIPAPDGAPVAYTEYSIDGGAWVKGTSVTLKRQGVTTVHYRSADTHDNVEQARTCTV
                     RIDRVAPVVFDYGRPVARQGGLLRCTYKVTDALSAKVTAKLVVKRVHTTKTKSYDLGL
                     RPTGRRLTTAVKCDLGVGTWAWRVQVRDHAGNMGHGPWRYLVVVRR"
     gene            complement(18694..18954)
                     /locus_tag="VFZ86_00575"
     CDS             complement(18694..18954)
                     /locus_tag="VFZ86_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5640811.1"
                     /translation="MIQAIAEALIYLATVVAIVGGIVFFACVLSLVLRERHERRAFLN
                     DCRRENAARRAGRTLESKPAPLGRVESPVPYPNAARALRRHA"
     gene            complement(19108..19818)
                     /locus_tag="VFZ86_00580"
     CDS             complement(19108..19818)
                     /locus_tag="VFZ86_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018885986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5640812.1"
                     /translation="MTISERPTRILVVDDEQQIRRALKSILSTRGYALEMAATAEEAL
                     LKAIDTPPDLVVLDLMLPDRSGIEVCRELRTWMTAPILILSVRSGEAEKIQALDEGAD
                     DYLTKPFSAGELLARIRALLRRAAAVTSPPPAVRAGDLEVDIARRRVRLAGSDVALTP
                     TEFDILAFLARNAGLVVTQRMILEQIWGPEWVEDAQTLRVHVSNLRKKIEPRSGGPRF
                     IITEPGVGFRFASAEDDD"
     gene            complement(19815..21350)
                     /locus_tag="VFZ86_00585"
     CDS             complement(19815..21350)
                     /locus_tag="VFZ86_00585"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HEX5640813.1"
                     /translation="MFSGIARAFSRHGYILAVTVIALATTLFLPGRDVFAKGQWALLY
                     LLVILFVASAAGAGPAILAAVLAFFTWNFFFLPPYHTLQIHESKDWLALGAFLVVGII
                     VGVQAGRMKEREARALARERETAVLNRLSAAVVTQTSTERMARSCVSEVAEAVHCASA
                     TLFLPQEGKLRGYCHVPADCPVDPSVAAHAERAFGTGGAGIAEAEVTDDGGLYVPVNS
                     PSGVSGVIAVQARADGRPYSAGDVRLVTSLGNLVGAFLERERLQAEATTAAAAREADR
                     LKSSLLSSVSHELKTPLAALTATVSNLLESDVDWDEDSVRDELRAIVDDVARLNNSIG
                     ALLELSRLEAHAWEPRLELYELSDIVAAAVDALPARMRGRVVQELPEDSPVVSVDFEQ
                     MKRAIQNLLENAIVHGRQDGDVSVGARVWHEGVRLWVEDQGPGVPPDEHEAVFEKFYR
                     GRPNEERVVSGTGLGLAIAREIVHAHGGAIVVEDVKPHGARFVIILSFERPEARSSAG
                     DEP"
     gene            complement(21527..21603)
                     /locus_tag="VFZ86_00590"
     tRNA            complement(21527..21603)
                     /locus_tag="VFZ86_00590"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21567..21569),aa:Ile,seq:gat)
     gene            21777..22079
                     /locus_tag="VFZ86_00595"
     CDS             21777..22079
                     /locus_tag="VFZ86_00595"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEX5640814.1"
                     /translation="MTVTELKAHLAEYLRRVREGERFHITSHGKEVADLVPPDPVRAA
                     LWDMVEAGEATWSGEDMVVPADLPLNTGRLLSDIVLEDRGPHWDEPDPAAADPGPE"
     gene            22076..22519
                     /locus_tag="VFZ86_00600"
     CDS             22076..22519
                     /locus_tag="VFZ86_00600"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX5640815.1"
                     /translation="MIVYLDTSAFVKLMVEEDGSAEVHSWFGEGWPAVSSVVTYPETC
                     SALGRRSHERGGHARLAPWLEELEMRWRRVLAIRVDATFAGRLAITHRLRGMDAVQLA
                     AAMITRERLRAADAAEELRFAVFDRELRAAAEREGFATLGGPLAT"
     gene            complement(22577..23668)
                     /locus_tag="VFZ86_00605"
     CDS             complement(22577..23668)
                     /locus_tag="VFZ86_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5640816.1"
                     /translation="MIPALTARGASAWHGIYEAQPTGTLVYTMTEAVPQMTLTATDAT
                     GLPVWSVETGDAWAAPGKLPAALVATFPDPDPNVLSDGELRAYDSRGMVQFRKKFENR
                     FVQPLCDTPKRLVWVEVSAKAVTRVFVRQGSTTHTVALPYQPRRAGFPMFAASSAAGS
                     RLIVGTSMPSTDRRRVMTCWLRVSRSGVPSIVSRGITDWVYVALSPRGDKAAVMTSEA
                     VGGVGNRWVSFGRFSGRLFEGEDSGEMDVSAHRIFVQGRYTFGDGQDGWSTHMVTVLD
                     HSPMMIYKRAWTFDDATSGIWFRCNGRIDRLAGVDDKGALTVVNVDSWAITTLPGPYA
                     DAVPIDGGRIATITGDGTLAFIADPVPGF"
     gene            24007..24663
                     /locus_tag="VFZ86_00610"
     CDS             24007..24663
                     /locus_tag="VFZ86_00610"
                     /inference="COORDINATES: protein motif:HMM:NF014618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HEX5640817.1"
                     /translation="MMDVQEMVIYGVSFDVIGKQPIVLLKTVEGNRFLPIWIGHPEAA
                     AILIKLQGTSLPRPMTHDLLTSVIGKFDAEVSRITVTELKESTFYARLTLKKDGEEVD
                     IDSRPSDALALAVRTDAPIFASTELIDENAIEFEREVDDTEEIVESFRDFLEGVSPDD
                     FRTAEVTADDEAFLIDHDADDEDDEDDDDDLDDEDAEDDDDDEEDDEFDDRAGGPPHN
                     "
     gene            24724..25014
                     /locus_tag="VFZ86_00615"
     CDS             24724..25014
                     /locus_tag="VFZ86_00615"
                     /inference="COORDINATES: protein motif:HMM:NF013939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEX5640818.1"
                     /translation="MSTPNDDPVIKQFRDQISDNDLKIIDLVNKRLSLVDKLWRYKAE
                     HGVDMYAPDREEWMLTFLSRANKGPLSQDALRDLYKTIVETTKSEAQRLGAG"
     gene            complement(25048..26481)
                     /locus_tag="VFZ86_00620"
     CDS             complement(25048..26481)
                     /locus_tag="VFZ86_00620"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006968637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HEX5640819.1"
                     /translation="MRIERTGEFVYEIPPEGQMRVPGRVYAHASLFDQAGHEEALQQV
                     ANVATLPGIVSASIAMPDIHWGYGFPIGGVAAMDEADGVVSPGGVGFDINCGVRLVRT
                     ALGLEDVRSRLEPLMHELMRRVPQGSGPHGALTLKEGELGRLAEEGAAFLARRGYATE
                     DDLLHTEEGGMMPGADSAAVSRRALERGRTQVGSLGSGNHFLEVQVVEEVLDERAASA
                     FGVGPGQVVVMIHSGSRGFGHQICTDHVGRMASAAARAGIELPDRQLACAPLGSPEAR
                     EYLAAMACACNFAFANRQMMLHEVRGAFERVFRASWNRLGVELVYDVCHNIAKREVHV
                     VDGVERAVRVHRKGATRAFGPGSPDVPPDYRDVGQPVIIPGDMGTESWLLAGTETAMR
                     ETFGSTCHGAGRLLSRHAARKVRSGAEVRRELEDAGIVVKAASVGGLAEEAPFAYKDV
                     ADVVAVVDAVGLSRKVARMRPLGVLKG"
     gene            complement(26511..26951)
                     /locus_tag="VFZ86_00625"
     CDS             complement(26511..26951)
                     /locus_tag="VFZ86_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HEX5640820.1"
                     /translation="MSAGYPSMSVGYELVDHTADIGVRVWGPTAEEVFEQAALATFSL
                     VCDPLDVGELTAVEVALEAESMDLLLAAWLNELLYVFEARRLVLTQFDIEDLSDTSLR
                     ARVSGEPLDPDRHIMCGGVKAATLHELSLERRDDGWEGFVLLDV"
     gene            complement(27024..27929)
                     /gene="thrB"
                     /locus_tag="VFZ86_00630"
     CDS             complement(27024..27929)
                     /gene="thrB"
                     /locus_tag="VFZ86_00630"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEX5640821.1"
                     /translation="MSCRVRVPATTANIGPGFDCLGLALDLWNEVRFSLEGDRVEVTV
                     EGPDPAGLPRDENNLVARAYLRLCEEAGKAPPAGLRIHCDIRVPMSSGLGSSSTAIVA
                     GLLGANALTGRPFDRDRILELAADIEGHPDNVAAALLGGLTIVVQKDDRLLTKKILVP
                     EVHVALAVPDLPFSTRAARAALPTEVSMADAVFNLQRTPLVVEALRSGDYELLSQVMD
                     DRLHQASRLKRIPGGRIAWLAALNAGAAAVAVSGSGPSLIAFVSLATDASRIARVMAE
                     AFAAEGVTAIPLGLAASAAGATVAV"
     gene            complement(27926..28576)
                     /locus_tag="VFZ86_00635"
     CDS             complement(27926..28576)
                     /locus_tag="VFZ86_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013074089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEX5640822.1"
                     /translation="MQSIIDILVQWATGLIGDWGLPAVFFLMLLESACIPVPSEAIMP
                     FAGFAVSEGDMTLLSITVAGVTGNVVGSWIAYAVGLYGGRPFIDKYGKYVLLRHHHVE
                     LAERWFAKYGAATVFFTRCLPIVRTFISLPAGIARMPFWKFTLYTLLGCIPWVFFLGW
                     LGVRLGEHWEDIRPYLHYADYAVVAALVAIVVWAFVRWRKSRDHGGPGPEEYGDEA"
     gene            28674..29771
                     /locus_tag="VFZ86_00640"
     CDS             28674..29771
                     /locus_tag="VFZ86_00640"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX5640823.1"
                     /translation="MAVGLMDIKGQYAELQDRIEDAVLSVLRSGRVILGPNVKGFEEE
                     AAAYMGTTGAVGVANGTDGLTIALRALGVGPGDEVITVPFTFYATPEAIAQAGAKPVF
                     VDILPDTLNMDPDAIKAAITPRTKAVMPVDLFGLPADADAIRKVAEAHGLSVVEDACQ
                     AWGATYDSRRAGSLGDLGVFSFFPTKNLGGFGDGGLVTGHSEVLVQRARELRFHGSKD
                     KKTFTDIGFNSRLDELQAAILRLELEVIDEWNGRRALVAEWYDEYLPDDVVRPAVPDG
                     LTHVYHLYMIRHPRRDDIAAACKERQVDCAAYYTTPMHLQPVFSDMGHKRGDLPNTDA
                     AAATNLALPMHPNLTEEQVKEVAAAVEAGLA"
     gene            29822..30916
                     /locus_tag="VFZ86_00645"
     CDS             29822..30916
                     /locus_tag="VFZ86_00645"
                     /inference="COORDINATES: protein motif:HMM:NF015940.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF354 domain-containing protein"
                     /protein_id="HEX5640824.1"
                     /translation="MRVWVDLTDAASVVFFAPIVRRLEDAGHTVTLTARRFAGADLVL
                     RRYGLGGVLTTRHRGGSLGTRAVGLVNRTAQLLGSASSGRFDVAAGSHASDFVLTAWT
                     LGIRQMTFLDPYRLRRGNLVNVRLVDEVAVPEAVPVAGLTSMGVPREKLFRYPGFKEE
                     YYLYDARPDPDALLRLGVERRRVVGVVRPAAPPARPPASEAAPADEAALVRVIAELAG
                     RRNVTLVVVARDQDQRRRLLALDLPAVLVPDGPVDGVGLLAAADFVVGGGGAMLREAA
                     ALGTPAYTLAPSAGPVEASLLGDGRLRRVQGPDDIALRKKDIRTALALPRDPWLFADR
                     LLELGSDHPRRQRLGRLLQDAADGQPPPLV"
     gene            31074..31976
                     /locus_tag="VFZ86_00650"
     CDS             31074..31976
                     /locus_tag="VFZ86_00650"
                     /inference="COORDINATES: protein motif:HMM:NF040786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism-associated LysR family
                     transcriptional regulator"
                     /protein_id="HEX5640825.1"
                     /translation="MDIRQLQLFCRIVDRKSFSLAADEMHITQPAASQQVRSLERELK
                     TVLLDRSRRTVVPTDAGQVLYRYGREILDLDEKARTEILDLGELVAGRIVVGASTGPG
                     EHVLPAMLTRFKGDFPGVTIALHVDDTHAVIERVLSREFEIGAVGAVTPRPELVAEPL
                     ARDEIVLVCAPTHPWASRDQVSLGDLLAEPQLVQQQGAGVRIVVEDHLRRAGVRPERL
                     NVVMEIGLMESAKQAAIAGGGVTFLSRWAIGPEVEHGTLAVVQIEGFRVLRDFYTVRS
                     KTRVLSRAAEALLAFFREQYAAAG"
     gene            32430..33452
                     /locus_tag="VFZ86_00655"
     CDS             32430..33452
                     /locus_tag="VFZ86_00655"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX5640826.1"
                     /translation="MTDRESTTAAMHAPDADLAMRSVATLDGEVFDPEIDDTIPPFRP
                     GPEPSGLSAGAWLKRWGLGLAGWGIFVLVVYLTAGEHLNGYLSMAGLLIGFLVGLTGM
                     GGGALMTPVLIFFFGFEPTMAIGTDVTYAAVTKIAGSWKHWRQGSVDIPLALWLALGS
                     IPASLIGVATIAYVKASYGGDLINGVLYKAIGSALMMVGVLLIVKVVMHVDAAHRREN
                     IRMTRRLKLMTVTLGLCTGFIIGFTSVGSGTLLAVFLILFFPLTTDRIIGTDIFHAMI
                     LLVITGLAQMSVGNVDLWMVASLLMGSIPGVLLGSQLTVKAPTRVLRSILAVVLFLSG
                     LALVAK"
     gene            33546..34298
                     /locus_tag="VFZ86_00660"
     CDS             33546..34298
                     /locus_tag="VFZ86_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020595596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphosulfolactate synthase"
                     /protein_id="HEX5640827.1"
                     /translation="MFEFLTLPERTAKPRESGLTHVLDKGYSLEQVRQFLEVSGEYVD
                     IVKLGWGTAVVTPNVKEKIALYQSFGVPVCFGGTFFEVCLRQGKFDEFLALVDECEME
                     CVEISDGTVAMDQEEKLAILRKLSKRYKVLSEVGSKDEAVVITPSTWIDSIKRELEAG
                     AWKVITEGRESGTVGIYRPTGDVKDGLLEEIRESFDITQLLFEAPIKKQQAWFITQFG
                     TNVNLGNIPPEEVISVETLRVGVRGDTLLTFH"
BASE COUNT         5380 a        12293 c        11842 g         4804 t
ORIGIN      
        1 cgcctgcctg gcagggcgct gctgccggcg cgcggctccc gggcttcggc cgcgcgtgcc
       61 ggtacgccgg ccggcggccg ttgccaccgg ggtggcagcc gcggcgggcg cgcccgtagg
      121 cgcgccgcaa tcgggacaga atctcgcgcc gtccgggacg tcggtcccgc attcctcaca
      181 ggccggcgcc ggcgccaggt ctctcaggtc ggagccgcac tcggggcaga agttggcatc
      241 ctcggcgtcg tggccgcact cggggcagta catgcatcac tccgtaggtg tcgaactgca
      301 gtgttgctac caagtatagg cagaatgacg cagggtgcat cccacggcag tgtgggatca
      361 gcgctggggt aagagacgag acggggatac agggtgccgc ggaacgcccg gcgaggagcc
      421 gggccgaacg caggctgctc tcagtgagcg atgagttgga tgacgagcgc gaggatcgcg
      481 aagcatccga cgatgcccgc gaggaagatg cgctccgcaa ggatgtggtc gtctcgcatg
      541 gctccaccgt ccgaggcggt tacggtcaca ctgcgactat accccataag catgagcttg
      601 tctccgtcaa aaggatgaga catcgcccca tctctaaggg ggagcacaga cctgagcccg
      661 ctcgaggacg gccgcgggtc accctcccga tggaggaccc gcatccccgg cgcgcgtcgc
      721 tgttcgcagc cagagaggca ccacgggggc ccaggccagg accaggacga gcagaaagac
      781 agccgtggtc cgcgggccga gcaggggcac gacgaccacg gccgccagcg cccccaggac
      841 gcgagcggtg ttgaaaccga ggtcgacggc cgtgaacacc cttcctctgt agccgtccgg
      901 cacggctcgt tgcgccagcg tgtcggcggg cacgcgcgtc cagccgtacg aggccccgag
      961 caggacggct cccgggatca gtgtggccgg cgccgccgtc acgaggacgg gcaccagggc
     1021 cagcgccgcg aggacgaagg cggccgccat gagcaggggg ccgggcatgc gcgagcgcat
     1081 gaggccggag gtgaggaacc cgaggaagac gcccacgcct ccggccccga ccagcccgga
     1141 gaacgtgagg aggccgccgc cgagcacgcc ggtgatcacc gccagggtcg tggcgatgac
     1201 catcacctgc aggaactggc cgaccgccac cgccaggacc ggcgccagcg ccgcgggcgt
     1261 gcggccgatg cgccggaacc cgtccaccag gtcggcggcg gcggcggcga gttgcctgcc
     1321 cacgccctcg tcgtggatct ccctgggagg gagcctgtcc gagaggcggg cgccgatcgc
     1381 ggacgcggcg agccaggcga cgcaagccaa ggcgatcgtc accgccgttc cgctcgccga
     1441 cgtgatgaga ccgccggcga agataccgcc gaacagcgct accgtgccgg ccacggacgc
     1501 caccatgttg gcggcgaaga gcctggcgtc cgccgtcgcc gtcgcctcgt ctccggcgcc
     1561 gacgatccga ggcacgaccg ccgtcgcggt cgcctggaag agccggttgg cggagaacac
     1621 cacgagcgtg ccggcgtagg ccagcgcggc gacgccgacg agcggcagca gaagaagcgc
     1681 gaacgccgcc ctgaccagcg gcagcacgac caggatgggg cggcgcgccc agcggtccac
     1741 gaacacgccg gcgaagggct cgaccagcga gaacgggagc agggtcagcg ccgtcgccag
     1801 cgcgaacccg cggagcgtgc tctgcgactc cggaaggaag acgacggtgt ccaggacggc
     1861 cgcgaggaac gcgccctccg cgacctggct cgcgaggcgg gccgcgagga ggacgcggaa
     1921 gtcccgggac ccgagcacgc gacgcacatg gtcgagcccg tcagccagcg atcctcccgc
     1981 ggtgtagccg atctcactcg gcctcgcggc aagtatccgt gagacgcgcc tcggagacca
     2041 gtgccgcggc gttgccgacg acggtgcggc cgcgcaccag accgccccgc gggaacaaga
     2101 ggacatcgcc acccaggagg tccgaccatg gccaagatcc tctgcgtcct gtatcccgat
     2161 cctgtggacg ggtacccgga cacgtacgcg cgcaccgacc tgcccgtcct cgaccgctac
     2221 ccgaacgggc agaccctccc cacccccaca ggcgtcgggt tcacgcccgg ccagttgctc
     2281 ggcagcgtct ccggcgagct cggcttgcgg gagtatctcg agcaacaagg tcacacgctg
     2341 gtcgtgaccg cggacaagga cggtcccgac tcggtcttcg agcgcgagct ggcggacgcg
     2401 gacgtcgtca tctcgcagcc gttctggccc gcctacctga ccgccgagcg catcgccggg
     2461 gcgcccaacc tgaggctcgc gatcacggcc ggtatcggct ccgaccacgt cgacctcgac
     2521 gcggccatgg aacacggcgt caccgtcgcc gaagtcacgt actccaacag catcagcgta
     2581 gccgagcacg tcgtgatgat gatcctcgcg ctggtgcgca actacatccc atcctatgag
     2641 tgggtgacca aaggcggctg gaacatcgcc gactgcgtgg ctcgctccta cgatctggag
     2701 ggcatgaccg tagggacgtt cggagccggc cgcatcggcc tcggcgtgct gcgccgcctc
     2761 aagcccttcg atgtggacct gcactacacc gacaagtacc ggctgagcga cgacgtcgag
     2821 caggagctcg gcctcacgta ccacgaggac gtgaggtcgc tggtccaggc ctgcgacgtg
     2881 ctctccatcc acacgccgct gcatcccgag acggaggggt tgttcgaccg tgagttgctc
     2941 gcgacgatga agcgcggcgc ctacctcatc aacaccgcgc gcggcaagat ctgcgaccgc
     3001 gacgccgtcg tggaagcgct cgagagcggg cagctggcag gctacgccgg cgacgtgtgg
     3061 tacccccagc cggcgccggc gtcccacccc tggcggacga tgccgcatca cggcatgacg
     3121 ccgcacatct cggggtcctc gctgccggcg caggcgcgct acgcatccgg cacccgcgag
     3181 atcctcgaga gcttcttcgc cggcacgccg atccgcgagg agtacctgat cgtcgacggc
     3241 ggccgcctgg ccggcaccgg cgcccacacg tacaccgccg gcgagtcggc ctcggacgac
     3301 tcggagaggg ccgcttgaac ggtggtggca cagaggtcag gcgcatcggc ggagggggcg
     3361 gctccacacc cgtggtgccg ccccctcgac gctcgctgtg ctcaggtggc gccgcagagc
     3421 gggtccgccg ggttccgcgc acagaacgcc gcgagagccg gcccggtgag ccgtccgcga
     3481 cggcggtcga tcgcgatcca cgcgacggcg ccgacgagca gcgccgcgag cgagaagagg
     3541 atcgcggtcc agacgttcga gtccgtcggt gtggaactgg cgcgaccgtc ggcggcggca
     3601 gacgcgatgc cgccgcgacc ctgcagggtc gcggcggcct gtgacgtcgt cgccgcggcg
     3661 ccgggttccg gaccggacag cgcgacggcc ccgccgccgg ctccgctgcc ggcgacgttg
     3721 tacgccgccg tcatctgtgc cgctatggcg cacacgagca gcgccgcaag cgtgagtcca
     3781 gcaagacggg cgaaccgtct ctgtagtgtg tcattcatcg tactcacctc cgggcgccgg
     3841 cagagcgatc agcgccggcc ccttcgctgg ctggcgggtc accccgcctc ctcacctcga
     3901 ggtaccctct gaggctgtcc gctggtacga gcgataaaca gtccggcagc catggatccg
     3961 gtcgcctggg aggccggccg gatcgcgcaa ggcccatcgc cccttgggcg gcctcggagg
     4021 gctaggatgg gggaggtgac cggaccgtct gacaccctcg agccgttccg cacggcgcac
     4081 gcccgggagc ttgccggctg ggccgcgacc gcggcggagg ctcgccggtg gggcggccag
     4141 gatgtggtct ggcccgtggc cgcctccgtc gtctccgcct ggcacgccga ccctggcgtg
     4201 cgaccgttcg tcctcgtccg cagcggcatg ctgctcggct acggcgaggt ctggatcgat
     4261 gaggaggagc gcgaggtcga gctcgcgcgg atcatcgtgc gacccgaacg ccggggacgg
     4321 gggctcggcc gcctgctcgt gaggctgctt ctcgagcagg cggcccagac cggccatcag
     4381 accgcgttcg tgcgcgtcgc accgggcaac gcggcggccc tggcctgcta ccgtaacgcc
     4441 ggcctcgccc cggtgagcga ggaggagcgg caggccttca acgagggaca gccgctcgag
     4501 tacgtgtggt tgcgccgcga gctgaggtga gatgcagtcg cggcgcaggc ggcctgaggg
     4561 ccacccgcgc cgcgactctg cctgatccgt caggccgacg ctctacagag cgcgtcgtcg
     4621 ggatgctgtg cgcagtacgc cgccgacggc tccgctgcgg agcggcggcg acggaagacc
     4681 gcccatgcgg cgatgcttcc ggccaggacg gccacggccg cgccgaccca gatccacgtc
     4741 ggcgtgcccg acgatccgga cgatgcgggc tgcgcctcgg ctgcgacgcc ggcggtcggc
     4801 gccgggatcg tagccgcgcc gcctccggca gcgccacctc tgcccgcggt gcccgcgagc
     4861 ggcgacgtcg cgacgctcat cgcagcgacg ccaccgcggc cctcggttcc cggcgccggc
     4921 gtcgcggaag cgctgccgga cgtgtcgctc gcggccgcta cagcttgcgc cccgagcgcg
     4981 agcacgagca gggacaggac caggagagct gcgacgcgca ccgtccggcg tcgtgatgat
     5041 gacttcatgg acacacctca ctcctcggcg ccggccgtca cggctccggc gctgctgctg
     5101 actacggtcg ccccgccagc ctcaccgcgg ggtcccattc agcgtctccc cggattcgag
     5161 aaccacgccg tgcggaatgt tgcccgagcc gtcacgctcc gatcaggcgc tcgatcccgt
     5221 cgagcatcag ctggaggccg aagtcgagcg gcgcgtagag ctcaccctcc acgggggcga
     5281 acgcccctcc ctccacggcg cggaacagcg ccggatagcg ccccgggtcg acgatcgtcc
     5341 gcagcagctc cccgaaggct gcggtcgcct cgtcgggagc gaacagcccc cgctgggcgc
     5401 cctcccggga ggtcagggcc agccgggccg tcgaggacac gtagccgctg agcagcagca
     5461 ggacggcgac cttgtggtac tcgctgagct ggacgtcccc gagtgcgcgc agcccgaggt
     5521 ccatccaggc gatctggctg ggagtcccgg ccctctcgat gaaccgcacc tcgtccagcc
     5581 acgggtgccg ctccatgatc gcgtactgct cacgcgtcca ccaggcgagg tcgtcgcgcc
     5641 agccgccgtg cgggatgtcg aggccgtcgg gcggccgcca cgccgagtcg tgcatgagga
     5701 ggagcagctc gtccttgctg gccacgtggc gatagagcga catcgtggtg aagccgaggc
     5761 tctcggcgac gcgcttcatg gacacggccg cgagaccgtc gccgtcggcg agggcgatac
     5821 cgctctcgac gatgcgctcg aggctcaggc cgagccgggg cttgctctcg agctgctcgc
     5881 gcatccagag cttctccaag ccagacggcg atctgtcgtt catgtcgtct cccacggttg
     5941 gcgtacctcg taaacagaag tctacttgac atgctgtgtg tatgccatat actcgtcgtg
     6001 tacatgatac acacaacgtg cgaggtgagc aagatgacag cggcgatctc cgtacaggga
     6061 ctggacaaga gcttcggctc caagccggcc ctcgatggac tcgacctcac ggtggcggcc
     6121 ggtgaggtcc acggctttct ggggccgaac ggcgccggca agacgaccac gctccgcatc
     6181 ctgctggggc ttctccgcaa ggactcaggg gccgcgactg tgctggggat ggacccctgg
     6241 cgcgacgcgg cggcgctgca ccgtcgcctc gcgttcgtgc ccggtgacgt gagcctctgg
     6301 cccaatctca ccggcggcga ggtgatcgac ctgctgggcg ccctgcgcgg cggcctggac
     6361 cggtcgcggc gcgccgacct gatcgagcgc ttccagctgg accctaccaa gaagtgccgc
     6421 gcgtactcca agggcaaccg gcagaaggtg gcgctcgtgg cggcgttcgc gtccgccgtc
     6481 gacctctacc tcctggacga acccacgtcc gggctggacc ccctgatgat ggccgtcttc
     6541 caggacgtgg tccgcgaggc ccacgccggc ggcggcacca tcctcctgtc cagccacatc
     6601 ctgtccgagg tggaggcgct gtgcgaccac ctcaccatca tccgcgccgg ccgcacggcg
     6661 gacaccggcg cgttcgagga cctgcgtcac ctcacgcgga ccacggtcgt cgtggagacc
     6721 gccgagccga tcgactcgct tgcgtggctg ccgggcgtgc acgacgccac catcgagcgc
     6781 aaccgcgccg agttctccgt cggccacgcc gatctgaacg gggtgctcga gcatctcgtc
     6841 gggctcggca tcaccaccct caccacctca ccgccgaccc tcgaggaact cttcctccgg
     6901 cactacgcag acacgtccgt cacccagctg gaagccgacc tggagcgtgt ggcatgaact
     6961 ccttcaccgg gaccacgaga ctcatccgtc tggcgctgcg ccgcgaccgc ttcgtgctcg
     7021 cggcctggct gctggtgctc gccggcctgc tggcgatgtt cacggcgatg ttcgtcgaca
     7081 gcctgccggc ccaggccgat gtgcttcggg agacgcggct gatggccggc aacgcggggt
     7141 tccgcatgct cagcctcgcc tcaggggcga gcgtcggcgc ctacgcgatg agccggacgt
     7201 gggtgacgtt cgcgctcctc gccgccgtca tgagcgtgct ctgcgtggtg cggcacacgc
     7261 ggcagaacga agagactggc cgcgccgagc ttctcggcgc cggggtcgtc ggcccggccg
     7321 ccggcctcac ggcggcggtg gtggtggcgc tgggcgccaa cgtcgcgctg gcgccgctgc
     7381 tcgggctggc gatgatcgtg ggcggccagc ccgtggcagg atccctcgcg gccggagcct
     7441 cgatcgccgc ggtgggcgcc gccttcaccg gtgtcgccgc gctgacgtcg cagctctcgc
     7501 ggtcggcgcg cggagccacc gggctggcga ccgccgcgct cgccgtcgcc ttcctcgtca
     7561 gcggcgtcgg gaacatgctc ggcggcgtgg acgccggcgg gaccgtcgcc ttcagcgcct
     7621 ggccgagctg gctctcgccg atcggctggg gctacgagat gcggccgttc ggcggcgacc
     7681 actggtggct gctcggcctg ttcgccgcct tcaccggcgt actgctcgct accgccggcc
     7741 ggctcgccgt ccggcgcgac ctcggcgccg gcgcgctgcc cgagcggacg ggccgcgccg
     7801 aggcctccgc cgcgctccgc ggcccgttcg ggctcgcctg gcggctgcaa cgcccggcct
     7861 tcctcggctg gctcgtcgcg ctggccggct tcgggctgat cttcggctcc gtgagcaaga
     7921 gcgcgcggac catgggcggg agcgcgcggg agatgtacga gcgcacgctc ggcaccggcc
     7981 agatgctcga cgcctggttc accatgctga tcgtgatggg cggcatcgcc gtctcgatct
     8041 acgttgtgca ggcgctgctg cgcatgcgcg aggaggaggc ccgcggccgg ctcgagccga
     8101 tcctctcgag cgcggtgggc aggacccgct gggtgctgag cttcgtgctc acgaccgcgc
     8161 tcggggggac cgtcctcctc cttgtcttcg cgaccgccgt cgcgctgacc gcagggctgg
     8221 cgctgggaga cagctccggt ctgctcctcg acctgaacgc cgctgcactg gccgagttgc
     8281 ccgccgtcct cgtcatcgcc gggctggtcg tcgcgctctt cgccgtgctg ccgcgccggg
     8341 ccgtgccggt ggcgtgggtg gtgctcgggg tggccgtcct cctgagcccg ctcttcgacc
     8401 tcgacgtgtc gcaatggctg gcggacgcct cgcccttcgc tcaccagaag gcgcccgcgc
     8461 aggagatcgg catggtcgcc gtcgtggctc tgctcggcgc ggcggcaacg ctggtcgcgg
     8521 cgggactggc ctggttccgg cgccgcgacc tctcggcggg gtgacaccgc cgcgtcttcc
     8581 ccgcacggcg acggggctcg ggtgccttgt acggcgcccg atccccgtcg ccgtccggac
     8641 cctccgcggc cgtcagctcc tcggcgtccg ccgtgacgcg ggggagacat agacattcgt
     8701 cccacgttct ccggcgccca cgcggccgat aggcatgatg agagcctgga gtcgcgttcc
     8761 gggcgaggcg gaagggacga acgtggagtg ccggcaagcg cagcgatcga gtgacccaca
     8821 gaggcgcgct cctcatctgg agacggccta tccgccgcag cgtgcgtcgg ccgcccggat
     8881 gcgcaagacg ctccgcgcct atctggccca gcaggatgtc gcggacggca tcgtctacca
     8941 ggtcgtgctc gccgccgatg aggcgttcac caacgccgtc acccacgccg gcagcgacga
     9001 cgtgatccgc gtctcggcgt gcgtgtctga ggacgcggcc ctcgtcgagg tgcacgatgc
     9061 cggcggcggc ttctcgcttg ccgcgtcgga cccggaggcg gtccccgact tgcggcggcc
     9121 gagcggccgg ggggtgttcc tgatcgagca cctgatggat gaggtcagcg tcgagtcggg
     9181 acgctgcggg acgaccgtgc ggatggtgcg acgcctcgcc tgagtgggcc ctctctcggc
     9241 catagcgcgg cgggcgcgtc tacctcagcc gggcgggccg tcctcctcca cctgctcgat
     9301 gatctcgtcc tcggcctcgt cgagcgtgtg cttgcggacc gtgggcacag cgaagtgcgc
     9361 gatgacgacg aggaacatgg cgacgctcgc ccacgagttg acgaacgaca agacgagcgc
     9421 ggcggtgaag acggccgggg cgatggcgaa gcgccgggtc accgatttga tggcggaggg
     9481 cggcacgagc ggctcgatca gatcgcggcg cttcgcccac caccacagga ggttgaaggc
     9541 gagggcgccg tagagggcca cgatgctgta gacgccggcc gcgagacctg cgctgtccgt
     9601 gcccaggtga gcggcgagga tcgccgtcga gaacggggtc agcgtggccg ccatgaggac
     9661 catgccgttg atcagcagga agtagtggtc gacacgctcg atgacgttga agacgttgtg
     9721 gtggctggcc cacatgatca ggatgttgat gaagctcacg agaaaggcga tgtaggtggg
     9781 ccactggctc aggagcgcct cgcgcagcgc cacggggtcg tgatacttct ccgccaccgg
     9841 ttgcgcgagg ttgagcgcga ggagggtgat ggcgatcgcg aagacgccgt cgctgaaggc
     9901 ctccgtgcgg ctggtctcgc ggaggaggat cgcccgcttg accggctcgg tcgtgtcgcc
     9961 gccggcctcc agttcaccgg tgccgtcgaa gccctgggcc tgcggatcgg gcgcgctcat
    10021 gccgggcttc taccccgaga acggcgcgcg gccaccgtgt cagcgtccca gcgcgtagta
    10081 cgtctcatac acgtgctgtg cccggcggcg ggcggtgtcc cagttccaga ggcgctccgt
    10141 gaaccggtcg ggactcaggc tccgcacgta gtcactcggg tggggatcga agagtgactc
    10201 cacgaggagc cggccgatgc cggcggagcc ggagatgccg tgagcgttgc agccgccggc
    10261 catcacgaac cccttgaggc ggcgactctc gccgatgatg aaccggccgt cgggcgtgaa
    10321 cgtcggccag cccttgccga cgcgcgtccg ggacgcgccc cgggcctcgg gcaggaggcg
    10381 cgcgtactgc tcctcgaagc cgtcgagcac gcgctggtcc gcctcgacga cgggcgcgtc
    10441 ttcctccagc ctgtagctcc gcgggtcggc gctcagggcg tgcgcctccc agccgccgag
    10501 ccgcaggcgc tccccggtcg cgcgaccgta cagggtcatc tcggggatgc ggaagcacgg
    10561 caggcccgga tgcagtccgc gcagcgggac ggtgtcgtag tactcgtgcc gcacgggcac
    10621 gatggggagc tcgagacccg cgaggcgggc gacatgatac gcgtgcgcgc ccgcggcgtc
    10681 gatggccatt tggcagcgga cctcaccacg gtcggtctgc accgcggcca cgcggccgtc
    10741 gctgcaagtg atccccgtgg ccgccgcccc cgtctcgaag cgcacgccca tgcgccggca
    10801 acggtgctcg tacgccttcg ccaccgcgag gggcgtcatg tacccgtcgg acgggcacca
    10861 gagcaccgcc tggacggcgc tgagatcgag cggcggccag aggcgctcgg cctcggctcg
    10921 gccgatcagg gcgacctcca ggccggcctg ctcgcagacg gcctgcagcc gccgcagttc
    10981 ctggacgcgc gagggagaga acgcgaggcg gatcgagccg acctcgtgcc agtcgggctt
    11041 catctcgggg tcccgctgca gctcgcggaa cgtgtcgacc gagtgcatcg cgagccgcac
    11101 gcgctccacg ctcgagcgca cctgcccgca gagcccggcg ccctgcgagg tggtgacctg
    11161 gccgacgtcg ccctcacgct cgacgagcag gatgtcgcgc ttccccgctt ccgccaggct
    11221 gtatgcgacg ccgcagccga tagcgccgcc tccgatgatg acgatctcgc tctcggcgaa
    11281 caggctcacg cctcgccccc tgctcctcgc cggccgggta ccgcgggtgc cccccaagaa
    11341 gtggttgaga gtataccggc gtgcgtgggg acaggggccg ggggcgaagg ggtcagtcag
    11401 gcgatgatgc cctgggctcg tcgcccggag gctcgacctc gcggtgcgcc tccttgccgg
    11461 acggcggagc cgaggtgaac acggcccgga gggcgtactc ggcgcggcag gcgttgcaca
    11521 ccgcgatcag ccggcgcctg tcctgcaccc acacggtcag gggccagccc ccgcggctcg
    11581 gcagctcgcg gccgcaacac tggcagctgt cgtactccgg caggtccccg gcgtcgtgct
    11641 cgctctggac gacgctgagc ccgtgctctt cggcgcaggc ggtgcaggcg gccacggggt
    11701 ggtcgacgac gtcgtcgagg acccacagga cgacgtcgac cccacgaccg cagaatatgc
    11761 acttccgcac agagtacccc cctcagagtg cccgctcgga ggtctcctag tatccgcatg
    11821 caccgccgtc gagagaagcc ctgaaggcaa ggttccgtcg cctcgcttgg ctaccccgga
    11881 gtcccatgcc gatgccgacc ggcctcagcc ggcagccagc cgccagagca ggaccccggc
    11941 gatgaccgcg ggcgtggaga cggcgaccag cagccgcgcc agcccgcggt cgaggtcgaa
    12001 gaccgccgcg acgaccatcg tgtagaaggc gacgggcatg gccgcgagca ggaggaagac
    12061 gccggggatg cggacgccgg caagccatgc cagcccaagc aggacgagcg gcatgaggcc
    12121 ggagcgcagg gcgacggtca cggcggtccg ccagaggtcg ccgcggtcgt ggcggagcgg
    12181 ctcgagcgag acggcaaggc cgacctgcag gaacccgacg aacccgaaca gcatcccgac
    12241 caccggcccg accggctcga ccagggtggt gatgtcgacg cccagcagac gcagggcgac
    12301 cgctcccact ccggcgacca ccggcgggtt gagcagccag ctgcgcacca cctgcctcat
    12361 gcccggggcc cggctccaga gcgaatcggg gcccgcgtag tgccgtccga ggcccaggat
    12421 gacgccctgg ctggggagga ggaactggaa ctcggtgaag accacggcca gcgccaggcc
    12481 cggaccgccg aagaccagcg tggccagggg gtagccgagg atcgccgtgt tgcccatggc
    12541 cgtggcgtac gcgagcacgc cggcctcgtc ctggctgcgc cctcccagcc ggccccagag
    12601 caggcccgcg aggagcgtgg cccatgcggc cgcgacgacg cacgcgaacg cggccgcaag
    12661 ctccggccgc accgcgacgg tggtgtagcc gtagaccacg gccagcggag cgagcaccca
    12721 gagcacgagc acgaacaaca ggtacgaggc gcgctgcggg ttgcgcagcc ggcgctgcag
    12781 cgcgactccc gctgcgaagc acaccagcca gcccgcgatc gtgatcagca ccgctggtcc
    12841 cggggtcggt gggctctgtc gcggcggggt cgcatgggcc gcgagtctct cacacgcgtg
    12901 ccggcgacgc agcgaacacc cggtccgcgg acggagacgc gatcggcggc tgaccgctgg
    12961 ccgggctagg cgaccatagg cagcagcgcc ggcgagccgg cgagctcagc catgagtccg
    13021 cccgtggtga ggatgcgatc caccctcgcc gcggtggcgc ctgccgtgta cagcggcttc
    13081 tcccggtggg ggtcgtagcc ggcggcgctg aacaggccct cgcagatgca gaagaagtgc
    13141 tcgttgtcgt acttggcggc gcactgggtg aaccgcccct tcgcgtcctt gagcagcacg
    13201 tagcctttgg cgcacctcgc cgggcgtccc cggacgagcg cctcctggta catcggagac
    13261 tcacgcagca cgcggaacgg caagccacag ggcgagccgg gcgcggtggc caccaccacg
    13321 tcctcggagc gcgcgcgcag gacggcctgc ttgtaggcgg gggatgcgct gctctcctcg
    13381 gtggcaagga agcgcgtccc catctggacg ccgtcggcgc cgagggcgag aaagcgacgg
    13441 atgtccgcgt gcgagtagat cccaccggcc acgatcaccg gcagatcgcc gtgagccgcc
    13501 gcgacctctt tgaccggcgg caggagccgc tcgagggtgt tctcctcgcg gtcgacctgg
    13561 tcggcgcgaa agccgaggtg gccgccggcc agcgggccct cgaggacgac cgcgtcgggg
    13621 cggacgtgct ggcgctccca gcgcctgcag atgaccgcga gcgcccgcgc cgacgagacg
    13681 atcgggatga gcgcggtact gcgcgccggc tgtatcgacg gcaacgagag cggcaagccg
    13741 gcgcccgaga cgatcgcgtc ggcgccggcg tcgagagcgg cgcgcaccga ggatgcgaag
    13801 tcacgctgga gcgcgaccat gatgttgacg cccgcgaagc caccgcgcgc cttggccgcc
    13861 tccacctcca gacgcacggc ctcatagatg cccaccttgc gcccgaggcg tctggagacg
    13921 atccggtcaa ggcctgcgct ggagatcaac cccaggccgc ccgcgaccgc caccgccgat
    13981 gccaagggag ccagtgacac gccgacgccc attccgccct ggaagatcgg cagagcgagc
    14041 tgcatgccct ggatggcgag gagcgggagc gaggagggag ggaccaacaa gaccgaaatc
    14101 atacgatatc gtgagcgaaa cggccacccg tccggtgtcg acccaccgca gaggaggcga
    14161 tcacagtgcc gagagacccg tggatcgcgg tcatcgccga cacgcacggg ctgctgccgc
    14221 agcgcgtgct cgacgcatgt gccggcgccg accacgtcat ccacgccggc gacgtcggct
    14281 cccgtgacgt cctcgaccgg ctgacgcagc tggcgccggt cagcgtcgtc cgcggcaacc
    14341 gcgaccgcga cgcatttggt ctccagttgc ccggcgaggc cctcggcgag gttgccggtc
    14401 tgcgcttcgc cgtcgtccac aagcgtcgcg acctcaaggc ggcgcacccg gacccacgcg
    14461 ccgagggcct cgggctgctc gtctacggcg actcgcaccg cgccgagatc cgctaccgcg
    14521 gccacgagcc gggcgggatc cccgtcctct ggctgaaccc gggcaccgcc agcgccccct
    14581 tgccgcacga ccctcgcccg agcatggcga gggtgatcgt gcgcgacggg cttcccgagg
    14641 cgcgcatcgt cttcttcgac tgagtcgtgg ccgaccaccg agaccgcgcc tcggcgatcc
    14701 tcccgatgcc ggtccactgg acacgagggg ctctttcgga aggtaccctt gacaggctcg
    14761 aagacctccc catagcctgc cttccacagc gcggacgcac gggggagtga cgatgcctgt
    14821 tctcagacgc accacctcgc tacccggcgc ccccgggcca ccggcccacg cacccccgcc
    14881 tcgtctctgc gccgcactcg accacagagg taggcacgcc ttgcctctga accgaacgtc
    14941 gctcacacag cacaacaagg gagcacgcag gtgacacgag tcagcaagat cacgttcctc
    15001 atcctcgtcg cgctcgtcgt ggtgttcggc ttcgccgcca ccgcgctcgc cgacggccgg
    15061 gtcatccaga acgacccgga caatccgatc aagttctcca tcgacggggc catcgccagt
    15121 ggcgccgtct ccacgccgga cctgtacatc ggcacagtgg ggtcctacct gggcgccgaa
    15181 gccgacggga gcacacccgt gttcaaggag ggattcggca agagccccta cgacatcctg
    15241 atcatgaacg acaaggtggg catcgtcctc gcggtcggga cgccggaccc gtggggctac
    15301 cccggcggat cgatcctcga cgccggccgc gtggagtccc tgccggccgg ggctacggac
    15361 ctgaagggcg ccaccttcgg tcccgacacc gtgctgaccg tccagttcct gttcaacacc
    15421 tgggacgcct gggcgccggc gaacaccggc atcgtctact gcgacctcgt gaagtacgac
    15481 ttcgccgcca aggccatcga caacgtcgat ggcgtccccg cggtgcaggt caagcgcaag
    15541 ttcgccgtgc cgtacaacga cggcggcgtc tcggtggcga gggatctgga cgtcatctcg
    15601 tactacagca tcccggccga cgaggaatac gcctacatgt tcgactcggt ggtcaacaac
    15661 ggcgtcgcat tctcggcgga gagcgccaac gaggtcagcc tctccaacaa gggcggcgac
    15721 ggcatcgaca ccaagacggt cgccgcgctc gctgcagcca acacgtacaa ctgggtcgcc
    15781 gacgaggccg gcgagccgac ccgggcgttc tccacgaccc tgatctcgcc cggcgacaac
    15841 ccgggctccg atgggcggct ccatccgctg gcgagcttca ccggcgccag gggatatcgc
    15901 gagctcgcct tcgcggaccc cggctacatc gtcggtgaga gccgcaccta cgagtcctac
    15961 ctcatgatcg acgacgagtg ctcgtggcag ggcgtcatcg acttctacgc cgcgtacaag
    16021 gatctcgaca ccttccgggt ggccggggac gtgaccgatc aggccggcgc tgccgtgccc
    16081 tacccggtca tcgtcatcta ccgtggcacg acgctgtacg gctgggtgat gggcgacgag
    16141 gccggtcact acaccgccga cctgccggac gaggccggcg cagaaccgta ccacctgcag
    16201 gtcgagaagt cgggcacgac ggcgggtcct gtgagcgccg acttcaccgc cgccacggtc
    16261 cccgcggagg ggatcgacct gcaggccggg gactacctcg ttccggtgaa gttccacttc
    16321 cgcgaccagg acgacgcgcc ggtctggggc agggtgtcgg tgggcacgac gccgaccatc
    16381 gccttcacgg gccagaactt cttcttctcc gacaatgcca cggatgggtc ggtgaacaag
    16441 ggtgaggtga cggccctcgt caagccgggc gactactccg cgacctgcta cggggaaggc
    16501 ttcggcttct actcgtacac gacggcgacg aacacctaca gccaggtcgt caccggcaac
    16561 acggcgacgg ataccgagcg caccgtcacg atcaccaaga cgctgagcgc gccggtcgac
    16621 tggttcggca tcgacaacca ccaccacggc gtgaggagcg acgccttctc gccgcccgag
    16681 gtcgtcgcaa aggcgcagac cacagctggg ctcgaggtgc tgacgctgga tgaccacgag
    16741 tacgtgatcg acaacatcca cgtctacaac tgggccagga agatggatgc ggcgggctat
    16801 atgccttcgg aagaggtgac gccgagctgg gcgcacttcg acgtcatgcc gctgaccgag
    16861 gcggcctatg cccgcttcct ggaccgcgac cagaagaacg gcatcgtcaa caccaacgcc
    16921 tcgctgcagg gcatcatgga cgacggccac aacgccggcg tcgccatcgg cgccaaccac
    16981 ccgaacagct cgtacgggct gttcctcgcc gacgacaacc agacggtccc cggcgggatg
    17041 accgacgact tcgacggcat cgaggcgcag ttccgcgtcg acacgctggg cgaagcgatg
    17101 agctactgga acgcctacat cacgggcggg agccatcgcg gcgtcgccgt caagcgcccg
    17161 cactacatct acgcgagcac cgacatccac gactccggcg gcaggacgac cacgggcgcc
    17221 aggcgctcct acgtcttcgt caaggacggc gccgagcaga gcgcgtccga cttcgaagcg
    17281 ttcagcctga agttcgcgag gagtcaggct gccggccaca gctacagctc gagcggcgtc
    17341 ttcgtcacgc cgacgagcgg caagatgtac ggcgacacgt accgggcgga cgctaccggc
    17401 agcttcaccg cgaccttcaa ggtctcggca ctcaacccca tcaccaacat ctacgtgttc
    17461 gggagcacgg gcaccggcac cgggaccgct ccgttcgcga tgacgaacct cgtctcgcag
    17521 accacgtacg ccggggacga tctgagcacg tcgaaggact tcacgctgac cgtggacgac
    17581 gtgagcggca agcagtggta cgcgatcgcc gccgtgtcga gcgacggcaa gcaggcgttc
    17641 accaatccca tctgggtgaa cggcacggac gtgacgacgc cggtgaccat caccgacctg
    17701 gacccgatca tcaccacccc ggacgcgcct gtggccggga acgtgatcga gcagccgccg
    17761 acggccatcc tgaccaccac cccgtggtgc gggttccgtc tggccgattg ggaactgatc
    17821 aacggcgact tcggctcggt ggcgcagaag ggcgtcgagt acgtcttcac gctgacgttc
    17881 tacgcgccga agggctacgt cttcgatccg gcactcagca cggacgggaa cttcggccgg
    17941 aaggtctccg aggacggcgc acggctgatc tacagagcga tctacacgac ggaaccaccg
    18001 acgaccacgg tctccgggtg ccctgcaggc tgggtccgtg gccccgtcac actggacttc
    18061 gcggcgatac cggcgccgga cggcgcgccg gtggcgtaca cggagtacag catcgacggc
    18121 ggcgcgtggg tcaagggcac gagcgtgacg ctcaagcgtc agggcgtgac cacggtccac
    18181 taccgctccg ccgacactca cgacaacgta gaacaggcca ggacgtgcac ggtccgcatc
    18241 gaccgcgtgg cccctgtggt gttcgactac gggcgcccgg tcgctcggca gggcgggctc
    18301 ctgcgctgca cgtacaaggt gaccgacgcg ctgtccgcca aggtcaccgc caagctcgtg
    18361 gtcaagcggg tccacactac gaagaccaag agctacgacc tgggcctccg gcccacgggg
    18421 cgccgtctga cgaccgcggt caagtgcgac ctcggcgtcg gtacctgggc ctggcgggtg
    18481 caggtgcgcg accacgccgg caacatgggt cacggcccgt ggcgctacct ggtggtcgtt
    18541 cgccgctagc ctgatcatct ccaaccgctg acgaagaagg gggcggcctc caggccgccc
    18601 ccttcttcgt tcgatacgcg gatcatgccg accgggcgac tccgccggcg cggtcgggcc
    18661 acgccgcaag aacggggcgc agcccgcagg ctgtcaggcg tgtcgacgga gcgcgcgcgc
    18721 agcgttcggg tacggcaccg gactctcgac cctgccaagg ggagcaggct tcgactcgag
    18781 ggtgcgtcct gcccggcggg cggcgttctc gcgacgacag tcattcagga acgcgcgccg
    18841 ttcatgccgc tcccgcagga cgagggagag gacgcaggcg aagaagacga tcccccccac
    18901 gatggcgact acagtcgcga ggtagatcag ggcctctgcg atcgcctgga tcatgatgtg
    18961 cctccggcat tgcgcgcggc ccgcaggaca gcgagcctgg tcagaggatg cgcgcaggcc
    19021 catcaagaac gcgtgaatcc gagaggcggc gggctaaaga gtccgcaaac acggcgcggc
    19081 cggccttcgc caggagcgtc gcgaccgtca gtcgtcgtct tcggcggagg cgaagcggaa
    19141 gccgacgccg ggctcggtga tgatgaaccg cgggccgccc gagcggggct cgatcttctt
    19201 acgcaggttg ctgacgtgga cacgaagggt ctgggcgtcc tccacccact cgggtcccca
    19261 gatctgctcg aggatcatcc gctgcgtcac gacgaggccg gcgttgcgcg ccaggaacgc
    19321 gaggatgtcg aactcggtcg gcgtcagggc gacgtcggag ccggcgagcc ggacgcggcg
    19381 gcgggcgatg tccacctcga ggtcgcccgc cctgacggcc ggcggcggag acgtgactgc
    19441 agctgctcgg cgcagcaggg cgcggatgcg cgccagcagc tcgcccgccg agaacggctt
    19501 cgtcagatag tcgtcggcgc cctcatcgag ggcctggatc ttctcagcct cgccggagcg
    19561 caccgagagg atcaggatgg gcgccgtcat ccaggtgcgg agctcccggc agacctcgat
    19621 gccgctgcgg tccggaagca tcaggtcgag gaccacgagg tccggcggtg tatcgatggc
    19681 cttcaagagc gcctcttcgg cggtggcggc catctccagc gcgtagcctc gcgtcgagag
    19741 gatcgacttc agggcgcgtc tgatctgctg ctcgtcgtcg acgacgagga tgcgtgtcgg
    19801 ccgttctgag atcgtcatgg ctcatcccct gctgatgatc gggcttctgg ccgctcgaag
    19861 gacaggatga tcacgaaccg ggcgccgtgg ggcttcacgt cctctacgac gatggcgccg
    19921 ccgtgagcgt gcacgatctc gcgagcgatc gcgagaccga ggccggtccc ggagaccact
    19981 cgctcctcat tcgggcgacc gcggtagaac ttctcaaaga ccgcctcgtg ttcgtcgggc
    20041 ggcacccccg gtccttggtc ttccacccag agacgcacac cctcgtgcca cacccgggcg
    20101 ccgaccgaca cgtcgccgtc ctggcggccg tggacgatcg cgttctcgag cagattctgg
    20161 atggcccgct tcatctgctc gaagtccaca gagacgaccg gcgagtcttc gggcagctcc
    20221 tgcacgaccc gtcccctcat ccgcgcgggc agcgcgtcga cagcggcggc gacgatgtcg
    20281 ctgagctcgt agagctccag cctcggctcc cacgcgtgcg cctccaggcg tgagagctcc
    20341 agcaaagcgc cgatgctgtt gttgaggcgc gcgacatcgt cgacgatcgc gcgcaactcg
    20401 tcgcgcacgc tgtcctcgtc ccagtcgacg tcgctctcga gcaggttgct caccgtggcg
    20461 gtgagcgccg ccagcggcgt cttgagctca tgggagacgg aagagagcag actggacttg
    20521 agccggtcgg cctcccgggc agcggcggcc gtcgtggcct ccgcctgcag gcgttcgcgc
    20581 tccaaaaagg cgccgacgag attgccgagc gaggtcacca ggcggacgtc gccggccgag
    20641 tacggacggc cgtccgcccg cgcctgtacg gcgatgacgc ccgagactcc cgatgggctg
    20701 ttcaccggca cgtacaggcc gccgtcgtcc gtcacctccg cttcggcgat ccccgctcca
    20761 cccgtcccga aggcgcgctc ggcgtgtgcc gcgacggacg ggtccaccgg gcagtcggcg
    20821 ggcacgtggc agtagccccg aagcttgccc tcctgcggca ggaagagcgt cgccgacgcg
    20881 cagtgcacag cctcggccac ctcagagacg cagctcctcg ccatccgctc cgtggacgtc
    20941 tgggtgacga cggccgcgct gaggcgattg agcaccgccg tctcccgctc ccgggccagg
    21001 gcccgcgcct ccctttcctt catccgtccg gcctggacgc ccacgatgat cccgaccacc
    21061 aggaatgccc cgagcgccag ccagtccttc gactcgtgga tctgcagcgt gtggtacgga
    21121 ggcagaaaga agaagttcca ggtgaagaac gcgaggaccg ccgccaggat ggccggaccg
    21181 gccccggcgg cgctcgccac gaacaggatc accagcaggt agaggagcgc ccattggccc
    21241 ttggcaaaga cgtccctgcc gggaaggaac agcgtcgtgg caagggcgat gaccgtgacg
    21301 gcaaggatgt acccgtgacg cgagaaagcc cgcgcgatac ctgagaacat gccccgccct
    21361 cgccgccgat ggacggtgtc gattgtagcg gtcccgccgt gcgtcatcca cgacctctcg
    21421 atcaacggac ggagcgacac cccactgtca actgaagtgg tgagtgtctc ggtgaccgcc
    21481 ggactgccgg caggagaagc gcgaagagga ttccgcctgg gatcgctggt gggcgcacgt
    21541 ggattcgaac cacggacctc ttccttatca gagaagcgct ctaaccgact gagctatgcg
    21601 cccacagggg tcttggagtc tacgagcgcg ggcgcgtagc gtcaaccgcc cgcgaccagg
    21661 cacacgacca agcccacggg tgcgacgaaa aggtcaccct tgtgcttctg ttctgaccgg
    21721 tttacattcc atgtgatgac ttgttaagcc gtgggaaacg gaccatcatg gcgtctgtga
    21781 ccgtcactga actcaaagcg catctcgccg agtacctccg ccgcgtcagg gagggagaac
    21841 gcttccacat cacgtcgcac ggcaaggagg tggcagacct cgtgcccccc gatcccgtgc
    21901 gagccgcgct ctgggacatg gtggaagccg gcgaggccac ctggtcggga gaggacatgg
    21961 tcgtccccgc agatctgccc ctcaacacag gccgcctgct ctccgacatc gtgctggagg
    22021 accgtgggcc gcactgggac gagccggacc ctgctgctgc cgaccctggc cccgaatgat
    22081 cgtctacctc gacacaagtg cgtttgtgaa gctcatggtc gaggaggatg gatccgccga
    22141 ggttcactcc tggttcggcg agggctggcc ggccgtctcg agtgtcgtca cctatccaga
    22201 gacttgctcc gcactgggac ggcggtcgca cgaacgcggt gggcacgcgc ggctcgcccc
    22261 ctggctggaa gagctcgaga tgcggtggcg gcgcgtcctc gccatccggg tcgacgcgac
    22321 ctttgccggc aggctggcga tcacgcaccg cctgcgcggc atggacgcgg tccagctcgc
    22381 agcggcgatg atcacccgcg agcgcctgcg agccgcggac gcggcagaag agcttcgctt
    22441 cgcggtcttc gaccgtgagc tgcgcgctgc cgccgagcgc gagggcttcg ccacgctcgg
    22501 cggcccgctt gccacctgac gcggatggcc cgggaagggc ggcggcgaga agtctaccgc
    22561 caccctctcg ggcgtatcag aagccgggaa cggggtcggc gatgaaggcc agggtgccgt
    22621 ctccggtgat cgtcgcgatc cggccgccgt cgatcggcac ggcgtccgcg tacggccccg
    22681 gcagggtcgt gatcgcccag ctgtccacgt tgacgacggt cagggcgccc ttgtcgtcga
    22741 cgccggccaa ccggtcgatg cgaccgttgc accggaacca gatgccgctc gtggcgtcgt
    22801 cgaacgtcca ggcgcgcttg tagatcatca tcggcgagtg gtccagcacc gtcaccatgt
    22861 gggtgctcca cccgtcttgg ccgtcaccga aggtgtatcg accctggacg aagatccggt
    22921 gggcgctcac gtccatctcg ccgctgtcct caccctcgaa cagccgaccc gagaaccggc
    22981 cgaagctcac ccagcggtta ccgacgccgc cgacggcctc ggaggtcatg acagcggcct
    23041 tgtcgccgcg cgggctgagc gccacgtaca cccagtcggt gatgccgcgg gaaacgatgc
    23101 tgggcacgcc cgagcggctg acgcgcagcc agcacgtcat gacgcgccgc cggtcggtgc
    23161 tgggcatgct cgtgccgacg atcagccgtg aacccgcggc tgacgacgcc gcgaacattg
    23221 ggaagcccgc tctgcgcggc tggtagggca gggccaccgt gtgcgtggtg gagccctgcc
    23281 gcacgaacac gcgggtgacc gccttcgccg agacctctac ccacacgagg cgcttcggcg
    23341 tgtcgcacag gggctggacg aacctgttct cgaacttctt gcggaactgc accatgccgc
    23401 gcgagtcgta ggcccggagc tctccgtcgc taaggacgtt gggatctgga tccgggaatg
    23461 tggcgacgag ggcggccggc agcttgccgg gcgccgccca ggcatcaccg gtctcgaccg
    23521 accagaccgg caggccggtg gcgtcggtcg cggtgagcgt catctgcggg acggcttcgg
    23581 tcatcgtgta gacgagcgtg cccgtgggct gcgcctcgta gatgccgtgc cacgcggagg
    23641 cgccgcgcgc ggtgagggcg gggatcatgc ccggctcccc cgccgtcagg acttccggac
    23701 ttacccggcc gctgcgtgcc gacgcagccg cagcgagaac gagcgtgagg acgacgacgg
    23761 caaggagcag gacggacgtg aggacagccc tcctgtggtg tgacgatcca gatgcgcgcg
    23821 agctcgtcat ggcccctccc caggtctcgc gcgggccgcg gcccggcggg ctccgcaccg
    23881 ccagccggcg cgaaggtctc cacctccgat actactcccg acccgagggc gggcgtagcc
    23941 tgtcccgcgc acgccgggcg gggttacacg gtgtgagcct cctgaccctt gccgtatact
    24001 cagaccatga tggatgtaca ggagatggtc atctacggcg tcagcttcga cgtcatcggc
    24061 aagcagccca tcgtgctgct caagacggtg gagggcaacc gctttctccc catctggatc
    24121 gggcacccgg aggcggcggc gatcctcatc aagctgcagg gcacgagcct gccgcggccg
    24181 atgacccacg acctgctcac cagcgtcatc ggcaagttcg acgccgaggt ctcgcgcatc
    24241 accgtgaccg agctcaagga gagtacgttc tacgcccgcc tcaccctcaa gaaggacggc
    24301 gaagaggtgg acatcgactc gcggcccagc gacgcgctgg cgctcgcggt gcgcaccgac
    24361 gcgcccatct tcgccagcac cgagctcatc gacgagaacg ccatcgagtt cgagcgcgag
    24421 gtcgacgaca ccgaggagat cgtcgagagc ttccgcgact tcctcgaggg cgtctccccg
    24481 gacgacttcc gcaccgccga ggtcacggcc gacgacgagg ccttcctgat cgaccacgac
    24541 gcggacgacg aagacgacga ggacgatgac gacgacctcg acgacgaaga cgccgaggac
    24601 gacgacgatg acgaagaaga cgatgagttc gacgaccggg cgggcggccc gccgcacaac
    24661 taggcgcccc ccttctctgc cccccgttct cacaagaccc tccagcgacg aaaggcccac
    24721 accgtgagca cgcccaacga cgaccccgtc atcaagcagt tccgcgacca gatcagcgac
    24781 aacgacctca agatcatcga cctggtcaac aagaggctct cgctggtcga caagctctgg
    24841 cgctacaagg ccgagcacgg ggtcgacatg tacgctccgg accgcgagga gtggatgctc
    24901 acgttcctct cgcgcgccaa caagggcccg ctctcgcagg acgccctgcg cgacctctac
    24961 aagacgatcg tcgagacgac caagagcgaa gcgcagcggc tgggcgccgg ctgagcgtcg
    25021 ccgcgtggct gccgcgcccg gcgcccgtca ccccttgagc acgccgagcg gccgcatgcg
    25081 cgcgaccttg cgcgagaggc cgacggcgtc cacgacggcg acgacgtcgg ccacgtcctt
    25141 gtaggcgaac ggcgcctcct cggcgagccc gcccacggac gccgccttca ccacgatgcc
    25201 ggcgtcctcc agctcgcgcc gcacctcggc gccggagcgg accttgcggg ccgcgtgccg
    25261 ggagagcagg cggccggctc cgtggcaggt cgagccgaag gtctcgcgca tcgccgtctc
    25321 cgtgccggcg agcagccagc tctcggtgcc catgtcgccg gggatgatca ccggctggcc
    25381 gacgtcccgg tagtccggcg ggacgtcggg gctccccggc ccgaacgcgc gcgtggcgcc
    25441 cttgcggtgc acgcgcaccg cgcgctccac gccgtccacc acgtgcacct cgcgcttggc
    25501 gatgttgtgg cacacgtcgt agacgagctc cacgccgagc cgattccagc tcgcgcggaa
    25561 gacccgctcg aacgcgccgc ggacctcgtg cagcatcatc tgccggttgg cgaaggcgaa
    25621 gttgcaggcg caggccatgg cggcgaggta ctcgcgggcc tcgggcgagc cgagcggcgc
    25681 gcaggcgagc tgcctgtcgg gcagctcgat gccggcgcgc gccgccgcac tcgccatccg
    25741 gccgacgtgg tcggtgcaga tctggtggcc gaacccgcgc gagcccgagt ggatcatcac
    25801 gacgacctgg ccggggccga cgccgaaggc gctcgcggcc cgctcgtcga gcacctcctc
    25861 cacgacctgc acctcgagga agtggttgcc cgagcccagg ctcccgacct gcgtgcgccc
    25921 acgctccagc gcgcgccggc tcacggccgc cgaatccgcg ccgggcatca tcccgccctc
    25981 ctccgtgtgc agcaggtcgt cctccgtcgc gtacccgcgc cgggcaagga acgcggctcc
    26041 ctcttcggcg agccgcccca gctccccctc cttgagcgtc agcgcgccgt gaggacccga
    26101 gccctgcggc acccggcgca tcagctcgtg catcagcggc tcgagccgcg accgcacgtc
    26161 ctcgagccca agcgcggtcc gcaccaggcg cacgccgcag ttgatgtcga agccgacgcc
    26221 gcccggcgag accactccgt cggcctcgtc catcgccgcg acgccgccga tggggaagcc
    26281 gtagccccag tggatgtccg gcatcgcgat gctggcgctc acgatgccgg gcagcgtggc
    26341 cacgttggcc acctgctgga gcgcctcctc gtgcccggcc tggtcgaaga ggctcgcgtg
    26401 cgcgtacacg cgcccgggca cgcgcatctg gccttcgggc gggatctcgt agacgaactc
    26461 gccggtccgc tcgatgcgca tggccgcggc gccgccccgc cggccgcggc ctacacgtcc
    26521 aggaggacga agccctccca gccgtcgtcg cggcgttcca gcgacagctc gtgcagcgtc
    26581 gccgccttca cgcccccgca catgatgtga cggtccgggt cgagcggctc accgctcacc
    26641 ctggcgcgca gcgacgtgtc ggagaggtcc tcgatgtcga actgggtgag cacgagccgg
    26701 cgtgcctcga agacgtacag cagctcgttg agccacgccg cgagcagaag gtccatgctc
    26761 tcggcctcga gcgccacctc gaccgccgtg agctcaccga cgtcgagcgg gtcgcagacc
    26821 agcgagaacg tcgcgagggc ggcctgctcg aagacctcct cggccgtcgg accccagacg
    26881 cgcacgccga tgtccgcagt gtgatcgacc agctcgtagc cgacgctcat ggagggatag
    26941 cccgcgctca tggacggact gtacccgaag tggagccgga gccctccgat gacgtcccgc
    27001 agccgtcaga cggcgcgcag gcgtcagacg gccacggtgg cgccggcggc cgaggcggcg
    27061 aggccgaggg ggatcgccgt aacgccctcg gcggcgaagg cctcggccat cacgcgggcg
    27121 atgcgcgagg cgtcggtcgc gaggctgacg aaggcgatga ggctcggccc ggaccccgag
    27181 acggcgaccg cggcggcgcc ggcgttcagc gccgcgagcc aggcgatgcg gcctcccggg
    27241 atgcgcttga gccgggaggc ctggtgcagc cggtcgtcca tcacctggct gagcagctcg
    27301 tagtcgccgc tgcgcagcgc ctccaccacg agcggcgtgc gctgcaggtt gaagacggcg
    27361 tcggccatcg agacctcggt cggcagcgcg gccctggcgg cgcgcgtgct gaagggcagg
    27421 tcgggcaccg ccagcgccac gtggacctcg gggacgagga tcttcttggt gagcagacgg
    27481 tcgtccttct ggacgacgat ggtgaggccg ccgagcaggg ccgccgcgac gttgtcgggg
    27541 tggccctcga tgtcggcggc cagctcgagg atccggtcgc ggtcgaaggg ccggcccgtc
    27601 agcgcgttcg cgcccagcag gccggccacg atggcggtgg agctcgagcc gaggcccgac
    27661 gacatgggca cgcggatgtc gcagtggatg cgcaggccgg ccggcggagc cttgccggcc
    27721 tcctcgcaca agcgcaggta cgcccgcgcg accaggttgt tctcgtcgcg tggcagcccc
    27781 gccgggtccg gtccctccac cgtcacctcg acccggtcac cctccagcga gaaccggacc
    27841 tcgttccaca ggtcgagcgc gagccccagg cagtcgaagc cggggccgat gttggcggtg
    27901 gtcgcgggca cccggacgcg gcagctcatg cctcgtcccc gtactcctcg ggacccgggc
    27961 ccccatggtc gcggctcttg cgccacctga cgaacgccca caccacgatg gccacgagcg
    28021 ccgcgaccac cgcgtagtcc gcgtagtgca gataggggcg gatgtcctcc cagtgctcgc
    28081 cgaggcgcac ccccagccag ccgaggaaga agacccacgg gatacagccg agcagtgtgt
    28141 agagcgtgaa cttccagaac ggcatccggg cgatgccggc aggcagcgag atgaaggtgc
    28201 gcacgatcgg caggcagcgc gtgaagaaca ccgtcgccgc cccgtacttc gcgaaccagc
    28261 gctcggccag ttccacgtgg tggtgccgga ggagcacata cttgccgtac ttgtcgatga
    28321 acggccggcc accgtacaag ccgaccgcat aggcgatcca cgatcccacg acgtttccgg
    28381 tcacgccggc caccgtgatg gagagcagcg tcatgtcgcc ctcgctgacg gcgaagccgg
    28441 cgaaaggcat gatcgcctcg ctcggcacgg ggatgcaggc gctctccagc agcatcagga
    28501 agaagaccgc cggcagcccc cagtctccga tgagcccggt ggcccactgc acgaggatgt
    28561 cgatgatcga ctgcatgggc gaaagcgtac aggatgcgcg ctccggtagc ccccctccac
    28621 ggcgctgtgg taggttccgg gctcgacttc acccgccgca aggaggcgtc cgcatggccg
    28681 tcggactcat ggacatcaaa ggacagtacg ccgagctcca ggaccgcatc gaggacgccg
    28741 tgctctcggt gctgcgcagc gggcgggtga tcctggggcc gaacgtcaag gggttcgagg
    28801 aagaggccgc ggcctacatg ggcaccaccg gagccgtcgg cgtcgccaac ggcaccgacg
    28861 gcctcacgat cgccctccgc gccctgggcg tcgggccggg cgacgaggtc atcaccgtcc
    28921 cgttcacctt ctacgccacg cccgaggcca tcgcccaggc cggcgccaag cccgtgttcg
    28981 tcgacatcct gccggacacc ctcaacatgg atccggacgc gatcaaggcg gccatcaccc
    29041 cgcgcaccaa ggcggtcatg ccggtcgacc tcttcggcct gccggccgac gccgacgcca
    29101 tccgcaaggt cgcggaggcg cacggcctct ccgtcgtcga ggacgcctgc caggcgtggg
    29161 gcgcgaccta cgacagccgt cgcgccggca gcctcggcga cctcggcgtc ttcagcttct
    29221 tccccaccaa gaacctcggc gggttcggcg acggcggcct ggtcaccggg cacagcgagg
    29281 tcctggtgca gcgcgcccgc gagctccgct tccacggcag caaggacaag aagacgttca
    29341 ccgacatcgg cttcaactcg cgcctcgacg agctgcaggc ggcgatcctc cgcctcgaac
    29401 tcgaggtcat cgacgagtgg aacgggcgcc gggcgctcgt ggccgagtgg tacgacgagt
    29461 acctgccgga cgacgtcgtg cggcccgccg tccccgacgg gctcacgcac gtctaccacc
    29521 tctacatgat ccggcacccg cggcgcgacg acatcgccgc cgcctgcaag gagcgccagg
    29581 tggactgtgc ggcgtactac accacgccca tgcacctgca gccggtgttc tccgacatgg
    29641 ggcacaagcg cggcgacctc cccaacaccg acgccgccgc ggcgaccaac ctcgccttgc
    29701 cgatgcaccc caacctcacc gaggagcagg tcaaggaagt ggccgcggcg gtggaggccg
    29761 ggctggcctg acgaggatcc gcgccggacg tccgggccga cccctctcca cctccgccgg
    29821 catgcgcgtc tgggtcgacc tcaccgacgc cgcgtcggtc gtcttcttcg caccgatcgt
    29881 ccgccgcctc gaggacgccg ggcacaccgt cacgctcacg gcgcgccgct tcgccggcgc
    29941 cgacctcgtg ctgcggcgct acggcctcgg cggcgtgctc accacccggc accgcggcgg
    30001 cagtctcggc acgcgcgccg tcggcctggt caaccggacc gcccagctcc tcggctccgc
    30061 ctcgagcggc cgcttcgacg tggcggccgg cagccacgcc agcgacttcg tgctcaccgc
    30121 ctggacgctc gggatccggc agatgacgtt cctcgacccg taccgcctca ggcgcggcaa
    30181 cctcgtcaac gtccgcctcg tcgacgaggt cgccgttccc gaggcggtgc ccgtcgccgg
    30241 cctgacctcc atgggcgtgc cccgggagaa gctcttccgc taccccgggt tcaaggagga
    30301 gtactacctg tacgacgccc ggccggaccc tgacgcactg ctccggctgg gcgtcgagcg
    30361 ccggcgcgtc gtcggcgtgg tgcgcccagc cgcgccgccc gcgcgtccgc cggccagcga
    30421 agccgcgccg gcggacgagg cggcgctcgt ccgggtgatc gcggagctcg ccggccggcg
    30481 caacgtcacc ttggtcgtgg tggcgcgcga ccaggaccag cgccggcggc tgctcgcgct
    30541 cgacctcccg gcggtactgg tgcccgacgg cccggtcgac ggcgtcggcc tgctggccgc
    30601 cgccgacttc gtcgtcggag gcggcggcgc gatgctgcgc gaggccgccg ccctcgggac
    30661 tcccgcctac acactcgcgc cgtccgccgg ccccgtggag gcgtcgctcc tcggcgacgg
    30721 ccggttgcgg cgcgtgcagg ggccggacga catcgcgctg cgcaagaagg acatacgcac
    30781 cgccctcgcc ctgccccgcg acccctggct gttcgccgac cggctcctcg agctggggag
    30841 tgaccatccc cggcgccagc gtctcgggcg cctcctccag gacgctgccg acgggcagcc
    30901 gccaccgctc gtctgagccg tgctccagaa gtaccgttgt acgccggcgc gtacgctcaa
    30961 cggtgctgcc ccggaatcga caccagtgcg ccctcggccc gaactcggcg cgacgtgcat
    31021 ctccctctag gcccgcgggt gcctgcgtcg actggatggg ataagctcag cttatggaca
    31081 tccgccagct ccagctgttc tgccgcatcg tcgaccgcaa gagcttctcg ctcgcggccg
    31141 acgagatgca catcacccag ccggccgcca gccagcaggt ccgcagcctg gagcgcgagc
    31201 tcaagaccgt cctgctggac cgctcgcgcc gcaccgtcgt ccccaccgac gcggggcagg
    31261 tgctgtaccg ctacggccgc gagatcctcg acctggacga gaaggcccgc accgagatcc
    31321 tcgacctggg ggagctcgtc gccggccgga tcgtggtcgg cgcgtcgacc ggcccgggcg
    31381 agcacgtgct cccggcgatg ctgacccggt tcaaaggcga cttcccgggc gtgaccatcg
    31441 cgctgcacgt cgacgacacc cacgcggtca tcgagcgcgt cctctcacgc gagttcgaga
    31501 tcggggcggt cggcgccgtg acaccgcgcc ccgaactcgt cgccgagccc ctcgcccgcg
    31561 acgagatcgt gctggtctgc gcgccgacgc acccgtgggc gtcgcgcgat caggtgagcc
    31621 tcggcgacct gctcgccgag ccgcagctgg tgcagcagca gggcgccggc gtgcgcatcg
    31681 tcgtcgagga ccacctccgt cgcgccggcg tccgcccgga acggctcaac gtcgtcatgg
    31741 agataggcct catggagtcg gccaagcagg cggccatcgc cggcggcggt gtgaccttcc
    31801 tcagccggtg ggcgatcggc cccgaggtcg agcacggcac gctcgccgtg gtgcagatcg
    31861 agggcttccg cgttctgcgc gacttctaca cggtgcgttc caagaccagg gtgctgagcc
    31921 gggccgccga ggcgctgctc gcgttcttcc gcgagcagta cgcggcggcc ggctgacact
    31981 cttcgcggcg gccggctgac actcttcgcg accgccgcct gacactcctc gcggccgcat
    32041 cctccccggc tgccgacggg gtgctctctt tccgtgtcta caacgtagcg acatctcggg
    32101 cgcctttggg gcagcagctc cgccgcgtat ctacaaagta gagacgccga gagaggaccc
    32161 tgaagtgagg acgggggcgc cgcggagtcg aggtcggcgc tcggcgccgg gtggcggggg
    32221 ccaaggcact ctgggacccg cacccttcat tgccatttcg tatgctccgg tcgtgccgca
    32281 taaggtctgc ttctgctcgc gagcgtgcgc cggggtgacg ccgggtgagg ccgctgcacg
    32341 attcgtggcc cttatttgac agataaggga gaggctccca tactcgcgcc caatctgacg
    32401 ccggaccgct ccggctgtgc tgaggacgga tgaccgacag agagagcacc acggcagcga
    32461 tgcacgcacc ggatgcggac ctcgccatgc gcagcgtcgc gacgctggac ggtgaggtct
    32521 tcgacccgga gatcgacgac acgatcccgc cgttccgccc cggcccggag ccctccgggc
    32581 tgtcggccgg ggcatggctc aagcgctggg gcctcggtct ggccggctgg ggcatcttcg
    32641 tgctcgtcgt ctacctgacc gccggcgagc acctcaacgg ctacctcagc atggccggcc
    32701 tgctgatcgg cttcctcgtc ggactgacgg ggatgggcgg cggcgcgctc atgacgccgg
    32761 tgctgatctt cttcttcggc ttcgagccga ccatggccat cggcaccgac gtcacctacg
    32821 ccgccgtcac caagatcgcc ggctcctgga agcactggcg ccagggctcg gtggacatcc
    32881 ccctggcgct ctggctcgcg ctcggcagca tccccgcctc gctcatcggc gtcgccacga
    32941 tcgcctacgt caaggccagc tacggcggcg acctcatcaa cggcgtcctc tacaaggcga
    33001 tcggctcggc cctgatgatg gtcggcgtgc tgctcatcgt gaaggtggtc atgcacgtgg
    33061 acgccgccca ccgccgcgag aacatccgca tgacgcggcg gctcaagctc atgaccgtca
    33121 cgctcggcct ctgcaccggc ttcatcatcg gcttcacgtc ggtcggcagc ggcacgctgc
    33181 tcgccgtgtt cctgatcctg ttctttccgc tgaccacgga caggatcatc ggcaccgaca
    33241 tcttccacgc catgatcctg ctggtcatca ccggcctcgc gcagatgagc gtgggcaacg
    33301 tcgacctctg gatggtcgcc tcgctgctca tgggctccat cccgggcgtg ctgctcgggt
    33361 cgcagctcac ggtcaaggcg ccgacgcgcg tgctgcgcag catcctcgcc gtcgtgctgt
    33421 tcctgagcgg tctggcgctg gtcgcgaagt agctgcgcgg cgcggcgccg gtcctcgccg
    33481 gggacggtct ggtaccatct agcgagcgcc gcgagaggcg gcgcgcgatc acgggaggat
    33541 gaccgatgtt cgagttcctg acgctccccg agcgcacggc caagccgcgc gagagcggtc
    33601 tcacgcacgt gctcgacaag gggtacagcc tcgagcaggt gcgccagttc ctcgaggtct
    33661 ccggcgagta cgtggacatc gtcaagctcg gctggggcac cgccgtggtc acgccgaacg
    33721 tcaaggagaa gatcgctctc taccagagct tcggcgtccc cgtgtgcttc ggcggcacgt
    33781 tcttcgaggt ctgtctgcgc cagggcaagt tcgacgagtt cctggcgctg gtcgacgagt
    33841 gcgagatgga gtgcgtcgag atctcggacg gcaccgtcgc catggaccag gaagagaaac
    33901 tcgccatcct gcgcaagctc agcaagcgct acaaggtcct cagcgaggtc ggcagcaagg
    33961 acgaggccgt ggtcatcacg ccgtccacct ggatcgactc catcaagcgc gagctcgagg
    34021 cgggcgcctg gaaggtcatc accgagggac gcgagagcgg gaccgtgggc atctaccgcc
    34081 cgaccggcga cgtcaaggac gggctgctcg aggagatccg cgagtcgttc gacatcacgc
    34141 aactcctctt cgaggcgccg atcaagaagc agcaggcctg gttcatcacg cagttcggca
    34201 ccaacgtcaa cctcggcaac atcccgcccg aggaagtcat ctcggtggag acgctgcgcg
    34261 tcggcgtgcg cggcgacacg ctgctcacct tccactgaac gccgccggac gcggcctcg
//
LOCUS       DASUMX010000014         8011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_579282, whole genome shotgun sequence.
ACCESSION   DASUMX010000014 DASUMX010000000
VERSION     DASUMX010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 8011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8011
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_579282"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1490)
                     /locus_tag="VFZ86_00665"
     CDS             complement(<1..1490)
                     /locus_tag="VFZ86_00665"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HEX5640828.1"
                     /translation="MNDPLARPRTPASGGFAVAAPLFVGADLGKVTTSLAVGELGTDG
                     ELRVVETRAERHLGDPLRPFLALYRDLDAGRLAGVAATGVYGERLGTPVAGGVPEEIA
                     QEQAVAWLFPDGPLNVVRVGGGGYSILTRDAGGHVRYEANERCSAGTGETVEGLCARL
                     GRSLEDAVALAEASPDGVTVTSRCAVFAKSELTHFANQGESHGRIFRGLFEGVARNVH
                     SLYDKNKIDGPVVLIGHGALIAPVAAGVAALSEAPVTVADQAGVFEALGALRFSASRA
                     AGSAGHGDGAAAAEDGPLAAPPAGFPDDPRTLVRPQRSRIGRLDPARLGPGSVQRLED
                     PRRADDAGGPGARAAVLGLDLGSTGAKAALVDAAGGAVLASVYRRTEGNPVEAAQALV
                     AEVTEMDPTPVVAVGLTGSGRDAAATVFRAVFPELDGRLVVQNEIVAHATAAAALDPD
                     GGRSLSIVEIGGQDAKFINVENGRVVDADMNRVCSAGTGSFLEEQAV"
     gene            complement(1569..2015)
                     /locus_tag="VFZ86_00670"
     CDS             complement(1569..2015)
                     /locus_tag="VFZ86_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5640829.1"
                     /translation="MAYNYQPKDPRGKLREVTTRWTASMRVAGRSPNRGVVFGDEPAR
                     REPQAKGWLVDDRRPGAAGDRYLIMEDGDVWCARAGEAGSPALSAAVAAWLEEPNDDL
                     VMLLARSLGDARLGGSGWLEADDDVECVADRRLERRGHGGPERRDG"
     gene            2157..2984
                     /locus_tag="VFZ86_00675"
     CDS             2157..2984
                     /locus_tag="VFZ86_00675"
                     /inference="COORDINATES: protein motif:HMM:NF018021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HEX5640830.1"
                     /translation="MTDDHELDPELERLLDDAWEALEDGDLRTVKRALRAARATAADD
                     PAVIELEASLAREQDEPERALEAYRRWSEIDPDDPEPWLGSAEIYVDFGEPGEAARLL
                     RELLAGPRLDPLDEADARHLLGIACEEKGDAKGMVKEWLATLRLDEANDGPTPRLSRT
                     EFERLAGAALDELPEEILARLGNVPVFVEDRPSEALVLEGLDPRTLGLFHGIAMPDQS
                     VLGPGPDVGVIHLFQRNLEREVPDDEELGEEIRVTVLHETAHYFGAGEQDMHRFGLN"
     gene            complement(3014..3616)
                     /locus_tag="VFZ86_00680"
     CDS             complement(3014..3616)
                     /locus_tag="VFZ86_00680"
                     /inference="COORDINATES: protein motif:HMM:NF037876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX5640831.1"
                     /translation="MRREERARLRRREMLAAYYQVLKDEGLQGASIAKIARRIDAPPS
                     LLIHYFGTKEQMTIELVDYLLEEYRQGYGDRLAAIADPRERLVAILDTFFGAEYHQLL
                     DDSVFYACFYISLRHPAVRKSYAALYETSLELVETTLGECMAAGYVAADDAHELAVTV
                     KALEEGYAFLIGGGADEAAKAEIGGALKKRALQLLGLSAA"
     gene            3805..5196
                     /locus_tag="VFZ86_00685"
     CDS             3805..5196
                     /locus_tag="VFZ86_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018559939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaminase"
                     /protein_id="HEX5640832.1"
                     /translation="MATIPSAARRARSCDRDHLHELIARERAAYVEAHPRSRELFGQA
                     AGSLLAGVPMSWMSMLTGAHPMYFAAARGNRITDVDGNTFIDFCLGDTGAMTGHSPQV
                     VLDAIDARLRGRGGITTMLPTEDAAHVGTELQRRFGLPFWQFSLTATDANRWVLRMAR
                     MVQKRPHVLVFSECYHGTVDETIVVVGPDGRAMAKPGNVGPQVDPTLTTRVVEFNDPE
                     ALRAALAPRDVAAVLMEPWMTNIGIVPPRPGFLEVVRELCDETGTLLINDETHTFSAG
                     VGGCTRALGLRPDIVTIGKAIASGVPMGAYGVTEELADRVLGDPDADLVDQGGVGGTL
                     AGNALASAAARATLEHVLTEENFTHMTALATRYTEGVQDVLDTYRVPWVIIQLGARAE
                     YRFTPAVPRTGNESRAVHDAELEEYMHLYTLNRGIFMTPFHNMALMCPETSRADVDRH
                     TEVFGEAIAELYG"
     gene            complement(5215..5652)
                     /locus_tag="VFZ86_00690"
     CDS             complement(5215..5652)
                     /locus_tag="VFZ86_00690"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEX5640833.1"
                     /translation="MAIISLTAEELQGRQLLDETGRAIGAISDLYVDDDGRPHWLLVD
                     TSFFPGFFSVIPVTDVLVTDKGLEVHYSMEKLTGAPNNMKDKDYAKHREGLLYDYYRL
                     EAPEGVEDATHHGEADKVLQPPSALCRFSTLSCENQPVDFNAE"
     gene            5921..7375
                     /locus_tag="VFZ86_00695"
     CDS             5921..7375
                     /locus_tag="VFZ86_00695"
                     /inference="COORDINATES: protein motif:HMM:NF012340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin like domain-containing protein"
                     /protein_id="HEX5640834.1"
                     /translation="MTTARRFAPALLAIALTALAALAAAPAPAAAAKPEQGPLNPAFV
                     EALHDPLVGLGLGRLPNPVEVHVGAAAQARAARFAAPPSYDLRAEGRLSAVKDQHPWN
                     TCWAFANTAALESKLMPAAAEPDFSEDNVVRRSGYFSTMDQRYDWGGWDFMAVAYFAR
                     WAGPVDESADPYDHAAGANGTRKHVQGVVMVPGRSHFTDNDLLKRLVLENGALSAGMY
                     FDPDRSPYSEYTDDTGLHATYYSPQRENENHGVTIVGWDDAYPRERFQGDETPDETPP
                     GDGAFLVRNTWGDDFGEGGYFWVSYYDGSFALDRGTGTWGGCTSYSDVQDVSNYSRIY
                     QYDKLGVNSQWGYGISRVWGANRFTAARTQYIAAAGFYTLSSSTRYEVWAGRRFSSLS
                     LRASGTAELPGYLTVPFAKKLRVYARRSFVVAVKLVSPNETYPMATERRISGWAPLAS
                     ARRGQSFTSRNGRAWTDVTRVRADMNVCIKAFAK"
     gene            7398..>8011
                     /locus_tag="VFZ86_00700"
     CDS             7398..>8011
                     /locus_tag="VFZ86_00700"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEX5640835.1"
                     /translation="MSAARPFRLRAVLFDFDGTLTHPGALDFAAIKRAVGCPPDQFVL
                     EWILALPPGAARDTATLALERFELEAADASAPNEGAEDLVRWLRGHGVAVGVLTRNGL
                     GAVERALRRFAALTVDDFDVVVTRDDEHVAPKPAPDGVLHAAAAMGVDPEETLVVGDF
                     LLDMRAGRAAGAVTAFLVNHGEPGAGEAAVPEAADCDFVVGRLG"
BASE COUNT         1149 a         2954 c         2827 g         1081 t
ORIGIN      
        1 accgcctgct cctcgaggaa cgaaccggtg ccggcgctgc agacgcggtt catgtcggcg
       61 tcgacgacgc ggccgttctc gacgttgatg aacttggcgt cctggccgcc gatctcgacg
      121 atcgagaggc tgcggccccc gtccggatcg agcgcggcgg cagccgtggc gtgggcgacg
      181 atctcgttct ggacgacgag ccgcccatcc agctccggga agacggcgcg gaacacggtc
      241 gccgcggcgt cacggcccga cccggttagg ccgaccgcca ccaccggggt cggatccatc
      301 tccgtgacct cggcgaccag cgcctgcgcc gcctcgacgg ggttgccctc cgtgcgccgg
      361 tagacgctcg ccagcacggc gccgccggcg gcgtcgacca gcgcggcctt ggcgccggtg
      421 gagccgaggt ccagaccgag caccgccgca cgcgcacccg gtccgccggc gtcgtccgca
      481 cgccgggggt cctcgaggcg ctgtaccgaa ccggggccca ggcgcgccgg atcgaggcgg
      541 ccgatgcggc tgcgctgcgg ccggacgagc gtccgcgggt cgtcggggaa gcccgcgggc
      601 ggcgccgcga gcgggccgtc ctcggctgcg gccgcaccat cgccgtggcc ggcgctcccg
      661 gcggcgcgcg acgcggagaa ccgcagcgcg ccgagcgcct cgaagacgcc cgcctggtcc
      721 gcgacggtga cgggggcctc gctcagggcg gcgaccccgg cggcgacggg cgcgatgaga
      781 gccccgtgcc cgatcaggac cacggggccg tcgatcttgt tcttgtcgta gaggctgtgg
      841 acgttgcggg cgacgccctc gaagagcccg cggaagatgc gcccgtggga ctcgccctgg
      901 ttggcgaagt gcgtgagctc gctcttggcg aacacggcgc agcggctggt gacggtgacg
      961 ccgtccgggc tggcctccgc caaggccaca gcgtcttcca gagaccggcc gagcctggcg
     1021 cacagaccct cgacggtctc gccggtcccg gccgaacaac gctcgttggc ctcgtagcgc
     1081 acgtgaccgc cggcgtcgcg cgtgaggatc gagtagccgc cgccgccgac gcggaccacg
     1141 ttgagcgggc cgtcggggaa gagccaggcg accgcctgct cctgcgcgat ctcctccggg
     1201 acaccacccg cgacgggcgt gccgagacgt tcgccgtaga cgcctgtcgc ggcgaccccg
     1261 gccagccggc cggcgtcgag gtcccggtag agcgcgagga acgggcgcag cggatcgccg
     1321 agatggcgct ccgcccgcgt ctccaccacg cgcagttcgc cgtccgtgcc gagctcgccc
     1381 acggcgagcg atgtggtgac cttgccgagg tcggcgccga cgaacagagg cgcggccacg
     1441 gcgaagccac cggacgcggg cgtgcgggga cgtgcgaggg ggtcgttcac gcctctgctt
     1501 ataccagaag ggggccgcgc acgctccgac ggccggccgt tcacggccgg cccggagctg
     1561 cccgccgctc agccgtcgcg ccgctcgggt cctccgtgcc cgcggcgctc cagccggcga
     1621 tcggcgacgc actcgacgtc gtcgtcggcc tcgagccagc cgctgccgcc gagccgcgcg
     1681 tcgccgagcg aacgggcgag cagcatcacc aggtcgtcgt tcggctcctc gagccacgcc
     1741 gccacggcgg cggacagggc gggcgatccc gcctcacccg cgcgggcgca ccagacgtct
     1801 ccgtcctcca tgatgaggta gcggtcgccg gcggcgccgg gccggcggtc gtcgaccagc
     1861 catcccttgg cctgcggttc ccgtcgcgcg ggctcgtcgc cgaacaccac gccgcggttg
     1921 ggggagcgtc cggcgacgcg catcgacgcc gtccacctgg tcgtcacctc gcgcagcttg
     1981 ccgcgcgggt cctttggctg gtagttgtac gccacgactc acctcctcga caggctgtcc
     2041 cgattgtgcc gacagaaggg tgacgattca aggtcttgtc gccgtgcaag gcgacacctg
     2101 tccggccacc tcgagggagg ctgtgccggg ggcgtccgcc ggggtacagt agcggcatga
     2161 ccgacgacca cgaactcgac ccggaactgg agcggctgct cgacgacgcc tgggaggcgc
     2221 tcgaggacgg cgacctgcgc accgtgaagc gggcgctgcg cgcggcgcgg gcgaccgccg
     2281 cggacgaccc ggcggtgatc gagctcgagg catcgctcgc acgggagcag gacgagcccg
     2341 agcgcgcgct ggaggcctac cgtcgctgga gcgagatcga ccccgacgac cccgagccgt
     2401 ggctggggtc ggccgagatc tacgtcgact tcggcgaacc cggcgaagcc gcgcgcctgc
     2461 tgcgcgagct gctcgccggc ccgcgactgg acccgctgga cgaagccgac gcgcgacacc
     2521 tcctcggcat cgcctgcgag gagaagggcg acgccaaggg catggtcaag gagtggctgg
     2581 ccacactgcg tctcgacgag gccaacgacg gcccgacgcc gcgtctctcg cggaccgagt
     2641 tcgagcgtct cgccggcgcc gccctcgacg aactgccgga ggagatcctc gcgcggctcg
     2701 gcaacgtccc cgtcttcgtc gaggaccgtc cgtccgaggc cctcgtcctc gagggtctcg
     2761 acccgcgcac gctgggcctg ttccacggca tcgcgatgcc ggaccagtcg gtgctcggtc
     2821 ccggtccgga cgtcggcgtc atccacctct tccagcgcaa cctggagcgg gaggtgcccg
     2881 acgatgagga gctgggcgag gagatccgcg tcaccgtcct gcacgagacg gcgcactact
     2941 tcggcgccgg cgagcaggac atgcaccgct tcgggctcaa ctagcgccgg cggccgcgac
     3001 cggccggcgg cggtcaggcg gcggagaggc cgagcagctg cagggcgcgt ttcttgagcg
     3061 cgccgccgat ctcggccttg gcggcctcat cggcgccgcc gccgatgagg aacgcgtagc
     3121 cctcctcgag agccttgacg gtcacggcga gctcgtgcgc gtcgtcggcg gcgacgtacc
     3181 cggcggccat gcactcgccg agcgtcgtct ccacgagctc gagtgacgtc tcgtacaggg
     3241 cggcgtagct cttgcgcacc gccgggtgcc gcaggctgat gtagaagcag gcgtagaaca
     3301 cgctgtcgtc gagcaactga tggtactcgg cgccgaagaa cgtatcgagg atggcgacca
     3361 gccgctcacg cgggtcggcg atcgcggcca gccggtcccc gtagccctgc cggtactcct
     3421 cgagcaggta gtccaccagc tcgatggtca tctgctcctt ggtgccgaag tagtggatga
     3481 gcaggctcgg cggcgcgtcg atgcgtctgg cgatcttggc gatcgacgcg ccctgcaggc
     3541 cctcgtcctt gagcacctgg tagtaggcgg ccagcatctc gcggcggcgc agccgcgccc
     3601 gctcctcgcg tctcaccggg ggatgcccac tctcgtatcg ccgcccatgc ggcgactcta
     3661 ccgtctcggc gcgatgcggc caaggagccg ttcaagcatt gaccgttcgt tcaactattg
     3721 actgaatact cgttcaagat tacgctccac taccgacgac ccgccacctc cgcacgcgga
     3781 gaggagccga cagaggaggg cctgatggcc accatcccga gcgcagcgcg gcgcgcacga
     3841 tcgtgcgacc gtgaccacct gcacgagctg atcgcccgcg aacgcgccgc gtacgtggag
     3901 gcgcaccccc gctcgcgtga gctgttcggg caggcggccg gcagcctgct cgccggcgtg
     3961 cccatgagct ggatgagcat gctcaccggc gcccacccca tgtacttcgc ggccgcccgc
     4021 ggcaaccgga tcaccgacgt ggacggcaac accttcatcg acttctgcct cggcgacacc
     4081 ggcgccatga ccggccactc cccacaggtc gtgctcgacg cgatcgacgc gcgcctgcgc
     4141 ggcaggggcg gcatcaccac catgctcccc acggaggacg ccgcccacgt ggggaccgag
     4201 ctgcagcgcc gcttcggcct gcccttctgg cagttctcgc tgacggccac cgacgccaac
     4261 cgctgggtgc tgcgcatggc gcgcatggtc cagaagcgcc cgcacgtcct cgtcttcagc
     4321 gagtgctacc acggcaccgt ggacgagacc atcgtggtcg tcggcccgga cggcagggcg
     4381 atggccaagc cgggcaacgt gggcccgcag gtcgatccca cgctgaccac cagggtcgtc
     4441 gagttcaatg acccggaggc gctgcgcgcg gcgctcgcgc cgcgcgacgt cgccgccgtg
     4501 ctcatggagc cgtggatgac caacatcggc atcgtccctc cccggcccgg cttcctcgag
     4561 gtcgtgcgcg agctctgcga cgagaccggc acgctcctga tcaacgacga gacgcacacg
     4621 ttcagcgccg gcgtcggcgg atgcacccgc gcgctcggcc tgcggccgga catcgtgacc
     4681 atcggcaagg ccatcgccag cggcgtgccg atgggcgcct acggcgtcac ggaggagctc
     4741 gccgaccgcg tcctcggcga ccccgacgcc gacctggtgg accagggcgg cgtcggcggc
     4801 accctggccg gcaacgcgct cgccagcgca gccgctcgcg ccacgctcga gcacgtgctc
     4861 accgaagaga acttcaccca catgaccgcc ctcgccacgc gctacaccga gggcgtacag
     4921 gacgtgctcg acacgtaccg cgtgccgtgg gtcatcatcc agctcggcgc ccgggcggag
     4981 taccgcttca cgcccgccgt gccccgcacc ggcaacgaga gccgcgccgt ccacgacgcg
     5041 gagctcgagg agtacatgca cctgtacacg ctcaaccgtg gcatcttcat gacgccgttc
     5101 cacaacatgg cgctcatgtg cccggagacg agcagggccg acgtcgaccg ccacacggag
     5161 gtcttcggcg aggcgatcgc cgagctgtac ggctgaggga ctcccgtcga gagcctactc
     5221 ggcgttgaag tcgaccggct ggttctcgca ggagagcgtc gagaagcggc agagcgcgct
     5281 cggcggctgc agtaccttgt ccgcctcgcc gtggtgcgtg gcatcctcca cgccctcggg
     5341 ggcttccagg cggtagtagt cgtacagcag gccctcgcgg tgcttggcgt agtccttgtc
     5401 cttcatgttg ttgggcgcgc cggtgagctt ctccatggag tagtgcacct cgaggccctt
     5461 gtcggtgacg agcacatcgg tcaccgggat gacgctgaag aagcccggga agaagctcgt
     5521 gtccacgagc agccagtgcg gacggccgtc gtcgtcgacg tagagatcgc tgatcgcgcc
     5581 gatggcgcgg cctgtctcgt ccagcagctg ccgtccctgc agctcctcgg cggtgagtga
     5641 gatgatcgcc atcgtccccc cctgcgatag tgcccgccgc gttgccgggc gaggtggtca
     5701 ccggatgggt gcgtcctacc cgcccggggg ccgcccggaa cgtagcgaag ggcgcgccgt
     5761 cacggcaacc cgcggtgccg gacccacccc ccggcccggc gccgggacgc cctgcgacgg
     5821 ccggggggtg cggagacgcg gcggcgcggc taggattcga tctgggccgc gcattgccga
     5881 caccggaagc gcccgcacgt cacaccgaga aaggcgccac atgaccaccg cccgccgctt
     5941 cgcccctgcg ctcctcgcca tcgcgctgac cgcactggcg gcgctcgccg cggcgccggc
     6001 gccggctgcg gcggccaagc cggagcaggg gccgctcaac ccggcgttcg tcgaggcgct
     6061 gcacgacccg ctggtcggcc tcggcctggg tcgcctgccc aacccggtgg aggtgcacgt
     6121 gggcgcggcc gcgcaggcca gagccgcgcg cttcgccgcc ccgccgtcgt acgacctgcg
     6181 cgccgagggc cgcctcagcg ccgtcaagga tcagcacccc tggaacacct gctgggcgtt
     6241 cgccaacacg gccgcgctcg agtcgaagct gatgcccgcc gctgcggagc cggacttcag
     6301 cgaggacaac gtcgtccggc gcagcggcta cttctcgacg atggaccagc gctacgactg
     6361 gggcggatgg gacttcatgg cggtggcgta cttcgcgcgc tgggccggcc ccgtcgacga
     6421 gagcgccgac ccctacgacc acgcggccgg cgccaacggg acgcgcaagc atgtccaggg
     6481 cgtggtcatg gtccccggcc gctcgcactt caccgacaac gacctcctca agcggctggt
     6541 gctggagaac ggggccctga gcgcggggat gtacttcgac ccggatcgga gcccatacag
     6601 cgagtacacg gacgacacgg gcctgcacgc cacctactac tcgccgcagc gggagaacga
     6661 gaaccacggc gtcaccatcg tggggtggga cgacgcctac cccagggagc gcttccaggg
     6721 cgacgagacg cccgacgaga cgccgcccgg agacggcgcc tttctcgtgc gcaacacctg
     6781 gggcgacgac ttcggcgagg gcggctactt ctgggtctcc tactacgacg gctcgttcgc
     6841 cctcgaccgc ggcaccggga cctggggcgg ctgcacctcc tacagcgacg tccaggacgt
     6901 gtccaactac tcccgcatct accagtacga caagctcggc gtgaactcgc agtggggcta
     6961 cggcatctcg cgcgtctggg gagccaaccg gttcaccgcc gccaggacgc agtacatcgc
     7021 cgccgccggc ttctacacgc tgtcctcgtc gacgagatac gaggtgtggg ccgggaggcg
     7081 cttctcgagc ctgagcctgc gggcctcggg gaccgccgag ctcccgggct acctgaccgt
     7141 ccccttcgcc aagaagctgc gcgtgtacgc gcggcgatcg ttcgtggtcg ccgtcaagct
     7201 cgtctctccg aacgagacct acccgatggc caccgagcgc cgcatcagcg gctgggcgcc
     7261 gctggcgtcg gcccggcggg gccagagctt cacgagccgc aacgggcgcg cctggaccga
     7321 cgtcacgcgc gtgcgtgcgg acatgaatgt ctgtatcaag gccttcgcca agtgaccgcg
     7381 agaacggccg cgccggcgtg agcgccgcgc ggccgttccg gctgcgtgcc gtcctcttcg
     7441 acttcgacgg cacgctcacg caccccggag cgctggattt cgccgccatc aagcgcgccg
     7501 tcggctgccc gcccgaccag ttcgtcctcg agtggatcct cgcgctgccg cccggcgcgg
     7561 cgcgcgacac ggcgacgctc gcgctcgagc ggttcgaact ggaggccgcc gacgcgtcgg
     7621 ccccgaacga aggcgccgag gacctggtgc ggtggctgcg cggacacggc gtggccgtcg
     7681 gcgtgctcac gcgcaacggg ctcggcgcgg tcgagcgcgc cctgcgccgg ttcgccgcgc
     7741 tcaccgtgga cgacttcgac gtggtggtga cgcgcgacga cgagcacgtc gcgcccaagc
     7801 ccgcgccgga cggcgtgctg cacgcggccg cggccatggg cgtggatccc gaggagacgc
     7861 tcgtcgtcgg cgacttcctt ctcgacatgc gcgccggcag ggcggcgggg gccgtgaccg
     7921 ccttcctcgt caatcacggc gagccgggcg ccggcgaagc ggccgtgccc gaggcggccg
     7981 actgcgactt cgtcgtcggc cgcctcggag a
//
LOCUS       DASUMX010000015        16076 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1010567, whole genome shotgun sequence.
ACCESSION   DASUMX010000015 DASUMX010000000
VERSION     DASUMX010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 16076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16076
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1010567"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="VFZ86_00705"
     CDS             complement(<1..594)
                     /locus_tag="VFZ86_00705"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018162198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX5640836.1"
                     /translation="MGHGSTDGDRPAAPATVVLDASGQWSADEGRIVERGLRSRTGVL
                     AAAANPVAQTVTVSYDPAVTSPRELRDWILRCGYHCAGESVPRHICAPLPDDSASAGA
                     PDTHEAAGAPETRHAGRHPDEAAGHAGHGGMSMAAMARDLRDRFIVAAVLAVPIMFWS
                     PMGTALLGRELPAPFGVSSEVFQFLLSLPVVFYSSAIF"
     gene            778..1197
                     /locus_tag="VFZ86_00710"
     CDS             778..1197
                     /locus_tag="VFZ86_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020522968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5640837.1"
                     /translation="MTDGRSHGTGTRDDPWILTTPPGKSEFSAYRDETADPPALVVTV
                     GKTELRYQLRCIEDLHAMLMRAGDWVPLGSADEQKPAAAGTVEAWGRSPDNPVGGWYG
                     LKKGLRGRFANYVPPVLEALGLAEVEHAARGNRMRAA"
     gene            1234..2595
                     /locus_tag="VFZ86_00715"
     CDS             1234..2595
                     /locus_tag="VFZ86_00715"
                     /inference="COORDINATES: protein motif:HMM:NF005453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaminase"
                     /protein_id="HEX5640838.1"
                     /translation="MDERAGVDPGRVDELWQRESARFAREHPRCLALRERARGLMPHG
                     VPMSWMASYFAHPPVWVDEGHGAHFTCADGRRFLDTNVGDKSTFCGIDPAPVVRAVQR
                     RVAAGAQFMLPTEDAIAVAEELARRWRLPSWQFTLSASQANTEALRLARHATGRRKVL
                     TFTGNYGGHGDEMLTAFAGEGRLSYLGLAPTADRDIDVIRFNDAGALERALAGGDYAC
                     VFTEPALTNEGVVLPEPGFHDALRALTRAAGTLLLLDETHTLICAPGGLTERWVLQPD
                     VVVVGKAAGGGLPLGAYGMTAELAAAFERGESLDGEPGELATGGTLFANALSMAAARA
                     VLTEVLTPAAYEHAAALGAELADGLEAVVDEARLPWKVQRLGPRSGISFSGRLARDAA
                     EADADEQPELNALLRLHLANRGVWEAISTAGPAMSVDATRDDVASYLAAFRTFVADVT
                     GSG"
     gene            complement(2657..3847)
                     /locus_tag="VFZ86_00720"
     CDS             complement(2657..3847)
                     /locus_tag="VFZ86_00720"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5640839.1"
                     /translation="MAAGATTDRAEARRRRRRAARRLALRYALLYALIAGAWIVLSDT
                     LLAWLGLPDDVELVLGSLKGIGFVVVTASLLMVFGLRYFRGIEASQERYTRLFENAVE
                     GLTVFKALRDRDGELTDLELVDVNPTQLARAGMAREEVVGKRLSRPDGLDQRTRSYLE
                     VVASAARSGSSARSELHVESADAYEFLEAYNVGRDRWALSALDITGVRHAERALRRQD
                     EDIRRAYVDVLGAVTGGKLILLTEEELAAQLGVPLMEQELVAAPRELAEARGAIARTA
                     SERFPDWAPRTDVRTPVGEALVNALRHAGGGSYQLFARDDRLQVLVRDDGPGIDFRTL
                     PKATLQRGFSTASSLGMGFTIMLQSCERVLLSTRPGRTSVVLEFADRPPLAESLESMA
                     VEAT"
     gene            3998..4495
                     /locus_tag="VFZ86_00725"
     CDS             3998..4495
                     /locus_tag="VFZ86_00725"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX5640840.1"
                     /translation="MTGAPHISAPDLEGLDDLSARTFRAFHRALRLHHRALMRAFARH
                     GIHHGQAMCLRLLASAPDVTQRDLARALHVSSPTVTRMVGSMEKAGLVRRRPDAADAR
                     LVRVELTDAGHAQERAMRAAAGEYVEHTFGALTEKERLELARLLEKLGDSIAQVAGEA
                     DEAAP"
     gene            4492..6225
                     /locus_tag="VFZ86_00730"
     CDS             4492..6225
                     /locus_tag="VFZ86_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018089807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5640841.1"
                     /translation="MIRLLRTGLRPYLWSIVLIVVLVFVQVLTNLYLPTLNADIINNG
                     VVKGDTAYIIEVGGIMLAVTLVSALAAIAAVYFGSRTSMALGRDTRGALFRRVQGFSQ
                     AEVNRFGAPSLITRTTNDVQQVQMVLLMMLNVVLMAPMMAIGGVVMALRLNVTLSTVI
                     LVAIPVMGVFIGLVISRATPLFMSMQKKIDRINQVTREFLSGVRVIRAFDRTAYEERR
                     FDVANEDLTDTSLKVGRLFALVFPAVTLIMNFTTIAIMWFGGKQIADGSMPIGDLTAF
                     LQYVFMILFSVLMATFMFVMVPRAAVSAQRIMEVLDTEPGIADPERPLPDVPEAGVVE
                     FRDVEFRYPGAEEPVLSGISFTARPGETTAIVGSTGSGKTTLISLIPRFYEVTSGAVL
                     VDGADVRELSQQDLWRRIGLVPQKSFLFNGTVAENLRVGDEQAADERLWHYLGIAQAR
                     DFVEEMPEQLETHIAQGGASVSGGQRQRLAVARALAKQAKIQIYDDSFSALDSSTDAR
                     LRAALHRHTAEATVIIVAQRVGTIMHADQIIVLDDDGTIAGIGTHGELLEQSETYREI
                     VTSQLGEEGVA"
     gene            6222..8228
                     /locus_tag="VFZ86_00735"
     CDS             6222..8228
                     /locus_tag="VFZ86_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007455540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5640842.1"
                     /translation="MSTPRDRSSPPSGPQAGVPKRPRGPQGPGPGGPGGHALVQPTER
                     AKDFKGTMRRLAGELRPERTLLILVVLLAVASVAFAVVGPKILGTATNILFEGVVSRQ
                     IPPGTTKEEAVAGLRAQGQDRIADMIGSMTLDPGEGIDFDALARILALLAGVYLVSAL
                     FQWLQSYLMAGIAQRAIYRLRRQADEKLARLPLKYFDDHPRGDVLSRMTNDIDNISQS
                     LQQSLTQIITSALTIIGVLIMMLTISWLLALISVLVVPVSVLITVTIAKRSQKQFARQ
                     WETTGDLNGHVEEMFTGHNVVKVFGRQEKAIATFEEQNEQLYQASFKAQFISGIIMPV
                     MNSVMNLNYVAIAVVGGLMVATGRISLGDVQAFIQYSRQFTMPITQVAGIMNVLQSTA
                     ASSERVFELLDEPEETPDPAECVVFEHTRGHIALEGVSFRYVPDTPLITDLTLDVAPG
                     ETVAIVGPTGAGKTTLVNLLLRFYDIDEGRILVDGVETRDISRSDLRRLFGMVLQDTW
                     LFSGTIRDNIAYGREGATEEEIRAAAKAARVDHFVRALPDGYDTVLDDDATNISQGER
                     QLLTIARAFLADPDILILDEATSSVDTRTEVLIQQAMAELMQDRTSFVIAHRLSTIRG
                     ADIILVMDRGAIIEQGSHAELMAARGFYHDLYASQFEEALDEAS"
     gene            complement(8275..9630)
                     /locus_tag="VFZ86_00740"
     CDS             complement(8275..9630)
                     /locus_tag="VFZ86_00740"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEX5640843.1"
                     /translation="MSTTGPDAPIAEAAAAEPAGGNGAALHGSPATPARRPGPQERRV
                     FVACVIAVLVYLVLVYFVFVRPGVDRADHVLAVVIPAAILGLSAELYPRLPAGLRASL
                     MLVFGVIGLVTGGVHVRRLGVEGVSASALCGLLPLAAGAVLVVLGVWLAWTSRKRGGP
                     LWWTVLRRALIAVAALFVVYWIVLPVSLAIVATERPNVPVPAADLGRPYEDVTLVTRD
                     GLELGAWYVPSQNRAAIVTFPRAWSTEQARMLVKHGYGVLLVDPRGYGESEGDPNAYG
                     WGSTPDIDAAVAWLRRRPDVQRRRIGGLGLSVGGEQMLEAAAGNSGLKAVVSEGAGIR
                     SVRESLLRRGPNALELALQYPQDLVQTVSVWVLSGEEVPMSLKNASLLISPRAVHFIY
                     GEEGQEVEKAVNPVYYDAAFPPKAIWEVPGSGHTRGIQTQPEEYERLVTGFFDRTLLE
                     Q"
     gene            complement(9667..10980)
                     /locus_tag="VFZ86_00745"
     CDS             complement(9667..10980)
                     /locus_tag="VFZ86_00745"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5640844.1"
                     /translation="MSAMGGEPAGDAGAATAGAAYRVYGYRWVVLGAFMFANLAIQIL
                     WISYAPITTSAAAYYGVSESTIGWFSMVFMIAFVPLSLPAAWLIDARGLRVAVGLGVV
                     LMAVFGVLRGLAGADFTLAMLATIGIAVAQPFLLNAWTTMGSHWFPARQRALAVGLIT
                     LANLVGTGIGVALTPLLIESRSVASVQLLFGVVGAVSAAAFLALARERPATPPCPPGM
                     EERALMLDGLRHALTVTPFLVYLGLVFVGMGVFNGVTTWVEQIVHPRGFSEADAGVIG
                     AAMLVGGLLGAVVIPALSDRHGRRVPYLVACFAFAIPGLLGVALFSSLWPLIAASLVL
                     GFFLVPALPVGMQYAAEVTWPTPEGTSNGLIQLFGQVAVVFVFVMSALRSEDGSFTAS
                     LLLASGLLLIAAVAASRLREPPPASERTAADSTAPDAPRPAAPVG"
     gene            complement(10977..12581)
                     /locus_tag="VFZ86_00750"
     CDS             complement(10977..12581)
                     /locus_tag="VFZ86_00750"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020487746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HEX5640845.1"
                     /translation="MVGTSSGSRHVLAVDLGTSGAKVALIGIDGRVVAWAFEPVELIV
                     LPGGGAEQRPEDWWRAVVAATRRLLARHDGPAARIAAVCCSAQGEGTVPVDETGAPLT
                     NCILWMDMRGAPHLRRQFGGPLSMHGLSLPRIRRWVKLTGGMPSPTGKDPAAHMLYVR
                     DELPEVYARTHKFLGVPDYLNLRLTGRMAASYDSIMTSWVTDNRDAGGVRYDAALVRD
                     CGVDAGKLPEIVKCTEVLGPLTPDAAEELGLAPETQVVAGAIDTTAAAVGAGATGDFA
                     VHLYLGTSSWLAAHVPFKKTDVIAGLASVPCALPGRWLLTALQATAGGNLTWLRDNVL
                     YHEDELLAEAEQPDVFKVFDQIAARVPPGSNGVLYTPWIWGERAPVDDRTLRAGLVNL
                     SLENTREDIVRAFFEGVALNTRWLLKPVRKFLGRPVDVVNLVGGGGQSDVWCQIVADV
                     LDVTVRRVRDPIQANARGAAWIAAAGLGEIAFSDVPRLVEFDGEFEPSPAARGVYDAR
                     YRTFLEVHRRMRPLYRRINRGGRQAR"
     gene            complement(12623..13645)
                     /locus_tag="VFZ86_00755"
     CDS             complement(12623..13645)
                     /locus_tag="VFZ86_00755"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HEX5640846.1"
                     /translation="MTGTQASGRGAQDRGRNTTTKQAKGLRAFLYDAKARDKEVELSE
                     GIAGRLTKDQLLWVDVDLASPAAVEQLRTLVEIDEGEFEEEDRLPVRERGGSFSFTIP
                     TLPSQEDDAELTPLTCIAAARWLVTAHQEEIEFLDAFYEHVHSDSSLGELDAPNFLAS
                     LLEWELNEFFLAMEREQRATDSIEEHILGDELARRSLDELVARRRRVSYLRSGLSPHR
                     YVYGTLAHASSDVVAGSDAAGDFALLGERVEQAVQAAQSARELIVGAFDLYMTRTAQR
                     TNDVMKVLTVISALLLPASLIAGIFGMNMLPDLFMNQWFFSVAIVVMVAVSGGLLTVL
                     RRRGYL"
     gene            complement(13712..14134)
                     /locus_tag="VFZ86_00760"
     CDS             complement(13712..14134)
                     /locus_tag="VFZ86_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX5640847.1"
                     /translation="MFLPDLNVLISAHRADSPMHPRCRDWLLAVYAGDESFGICPPVL
                     IGLVRILTHPRVFRPPDTHDQAFAFVNSLMTHPNALLLNPGRDHLALFEGLCREADAH
                     GDLVTDAYLAALALETGAVLVTADRDFARFPGLRRREP"
     gene            complement(14142..14408)
                     /locus_tag="VFZ86_00765"
     CDS             complement(14142..14408)
                     /locus_tag="VFZ86_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005000646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HEX5640848.1"
                     /translation="METIMRTTLTIDSQVLAEFKRRSAETHLTLSRLIEDALREHLSR
                     GRDVAPTKPVEFPIVGGRGLAPGVDLTSPSGMAEYLYGPDAGHR"
     gene            complement(14486..14842)
                     /locus_tag="VFZ86_00770"
     CDS             complement(14486..14842)
                     /locus_tag="VFZ86_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5640849.1"
                     /translation="MSTATAADAVIRAAGCRGPISVRPPHGKKLLSLPWELRARDRNT
                     VMWDLEPDSLDGIRDDAQAMTRYVVDNVRPASIVLLHPWAEGNAATCRALPAILAALQ
                     ARGFSFVTVSGLLALR"
     gene            complement(15072..>16076)
                     /locus_tag="VFZ86_00775"
     CDS             complement(15072..>16076)
                     /locus_tag="VFZ86_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007076461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5640850.1"
                     /translation="CEMFRMLGSGTESVMAAVRAARAFTGKKWVVKVGGAYHGWSDQM
                     VYGLHVPGTWRFEAKGIPFGATARTREVFPNDLDALRRKLRQNQSMGGTAAVILEPVG
                     PESGTRPVPFGFNAAVRELCDEFGALLIFDEVVTGFRLGLGGAQGYFGVRPDLTIFGK
                     CITGGYPMAGGVGGRRDVMASFAGGIGGKTGERAYVGGTLSANPLSCAAGYFAITEME
                     RTGAPVLAGMAGDRLTAGLRLLIEKYSLPFVAYNQGSIVHLETSGVMLLDLKHPLRFA
                     KQMKPRKHMMEEMGAAFTANGLVTLAGSRMYTSMADSDAVIDEALERFERVLASVEGV
                     "
BASE COUNT         2446 a         5804 c         5684 g         2142 t
ORIGIN      
        1 gaagatcgcc gacgagtaga agaccaccgg caggctgagg aggaactgga acacctcgga
       61 cgacacgccg aagggcgccg ggagttcgcg gccgagcagg gccgtgccca tgggcgacca
      121 gaacatgatg ggcacggcca ggacggccgc gacgatgaac cggtcgcgca ggtcgcgggc
      181 catggccgcc atggacatgc cgccgtggcc cgcgtgcccc gcggcctcgt ccggatgccg
      241 gccggcgtgg cgcgtctcgg gcgcgccggc ggcctcgtgc gtgtccggcg cgccggcgga
      301 tgccgagtcg tccggcagcg gcgcgcagat gtgccgcggc acggactcgc cggcgcagtg
      361 atagccgcac cggaggatcc agtcacgaag ctcacgcggg gaggtgaccg ccgggtcgta
      421 gctcaccgtc acggtctgcg ccacggggtt ggcggcggcg gcgagcactc ccgtccggct
      481 gcgcaggccg cgctcgacga tgcggccctc gtcggcggac cactgcccgc tcgcgtcgag
      541 gaccaccgtc gccggcgcgg ccggccggtc cccgtccgtc gacccgtgac ccatgctcag
      601 gtgcttcccc ttccgcaggc gccgcattcg ctgcgccgtg aggccgacca ggcgacacct
      661 cgcaggtcca ttcgacgcgg ccccgactcg gccgaagcag catggcgccg cgggacgccg
      721 ggaagaccgg ggacacggcc ggcagaacgc tgcggccgcg accggatcgg agcactcatg
      781 acagatggac gatcacacgg taccgggacc cgggacgatc cctggatcct caccacaccc
      841 ccgggcaagt cggagttcag tgcctaccgc gacgagaccg ccgacccgcc ggcgctcgtc
      901 gtgacggtcg gcaagacgga gcttcgctac cagctgcgct gcatcgagga cctgcacgcg
      961 atgctcatgc gcgcaggcga ctgggtcccc ctcggcagcg ccgacgagca gaagccggcg
     1021 gcggctggca cggtggaggc gtggggcagg tcaccggaca acccggtcgg cggctggtac
     1081 gggctcaaga agggtctccg cggccggttc gccaactacg tgccgccggt gctcgaggcg
     1141 ctcggcctcg ccgaggtcga gcacgcagcc cgcggcaacc ggatgcgcgc cgcctgaagc
     1201 gccgccgccg ggcgacctgc tagagtccac gccatggacg agcgggcggg cgtcgatccc
     1261 gggcgcgtgg acgagttgtg gcagcgcgag agcgcgcgct tcgcgcgcga gcacccgcgc
     1321 tgcctcgcgt tgcgcgaacg cgcgcgcggc ctgatgcccc acggcgtccc catgagctgg
     1381 atggccagct acttcgcgca cccgccggtg tgggtggacg aagggcacgg cgcccacttc
     1441 acctgcgccg acgggcgccg cttcctcgac accaacgtcg gcgacaagag caccttctgc
     1501 ggcatcgacc cggcgccggt cgtgcgcgcc gtgcagcggc gcgtcgccgc cggcgcccag
     1561 ttcatgctgc ccacggagga tgcgatcgcg gtggcggagg agctggcgcg ccgctggcgc
     1621 ctcccctcct ggcagttcac gctgtccgcc tcgcaggcca acaccgaggc cctgcgtctg
     1681 gcacggcacg cgaccggccg gcgcaaggtc ctcaccttca ccggcaacta cggcgggcac
     1741 ggcgacgaga tgctgacggc gttcgccggc gagggccggc tctcgtacct cgggctcgcc
     1801 cccaccgccg accgtgacat cgacgtgatc cgcttcaacg acgccggcgc gctcgagcgc
     1861 gccctcgccg gcggcgacta cgcttgcgtg ttcaccgagc cggcgctgac caatgagggc
     1921 gtcgtgctgc ccgaaccggg attccacgac gccctgcgcg cgctcacgcg cgcggccggc
     1981 accctgctcc tcctcgacga gacgcacacg ctgatctgcg cccccggcgg cctcaccgaa
     2041 cgctgggtcc tgcagcccga cgtcgtcgtg gtcggcaagg cggccggcgg gggactgccg
     2101 ctcggcgcgt acgggatgac cgccgagctc gccgcggcct tcgagcgcgg cgagagcctc
     2161 gacggtgagc cgggagagct cgccaccggc gggacgctgt tcgccaacgc gctcagcatg
     2221 gcggcggcgc gggcggtgct caccgaggtg ctgacgccgg cggcctacga gcacgccgcc
     2281 gccctcggcg ccgagctggc cgacggcctg gaggccgtcg tcgacgaggc tcgcctgccg
     2341 tggaaggtcc agcgcctcgg gccgcgctcg ggcatcagct tcagcggacg cctcgctcgc
     2401 gacgccgccg aggcggacgc ggacgagcag ccggagctca acgcgctgct gcggctgcac
     2461 ctcgccaacc gcggcgtgtg ggaggcgatc agcaccgcgg ggccggcgat gtcggtggac
     2521 gcgacccgcg acgacgtcgc gtcctacctc gcggcgttcc gcacgttcgt cgccgacgtc
     2581 accggatccg gctgagagac ccggcgcgcg ccgcgccgac ggcgcgcccg gccgcagcga
     2641 ggttgaccga ggcggcctac gtcgcctcga ccgccatgct ctccaggctc tccgcgagag
     2701 gcggccggtc ggcgaactcg aggaccacgg acgtgcgccc cggccgcgtg ctcagcagca
     2761 cgcgctcgca gctctgcagc atgatggtga agcccatgcc caggctggag gcggtggaga
     2821 agccgcgctg cagcgtggcc ttggggaggg tgcggaagtc gatccccggg ccatcgtcgc
     2881 gcacgagcac ctgcagccgg tcgtcgcgcg cgaacagctg gtagctgccg ccgccggcgt
     2941 gccgcagggc gttgacgagc gcctccccga ccggcgtgcg cacgtccgtc cgcggcgccc
     3001 agtccgggaa gcgctcggag gccgtgcgcg cgatcgcgcc gcgcgcctcg gcgagctctc
     3061 gcggcgctgc gacgagctcc tgctccatga gcggtacgcc gagctgcgcc gcgagctcct
     3121 cctcggtgag caggatcagc ttgccgccgg tgaccgcgcc gagcacgtcg acgtaggcgc
     3181 ggcggatgtc ctcgtcctgc cgccggagcg cccgctcggc gtggcgcacg ccggtgatgt
     3241 ccagcgcgga cagcgcccag cggtcgcgcc cgacgttgta cgcctccagg aactcgtagg
     3301 cgtcggcgct ctcgacgtgg agctcggagc gggcggacga gccggagcgg gccgcgctcg
     3361 ccaccacctc gaggtacgag cgcgtgcgct ggtcgagacc gtcgggccgg ctcagccgct
     3421 tgccgacgac ctcctccctg gccatgccgg cgcgtgccag ctgcgtcggg ttcacgtcca
     3481 cgagctcgag gtcggtgagc tccccgtcgc ggtcgcgcag ggccttgaac acggtgagtc
     3541 cctccacggc gttctcgaag aggcgcgtgt agcgctcctg agacgcctcg atgccgcgga
     3601 agtagcgcaa cccgaagacc atcagcaggg acgctgtgac gacgacgaag ccgatgccct
     3661 tgagcgaccc cagcacgagc tcgacgtcgt ccggcaggcc cagccacgcc aggagcgtgt
     3721 ccgagaggac gatccaggcg cccgcgatga gcgcgtagag cagggcgtac cggagcgcca
     3781 gccttcgcgc ggcacggcgc cgccgtctgc gcgcctcggc gcggtctgtg gtcgctcctg
     3841 cagccatcat ctcgtcgact cggggattcc ccggcgacac gccggaccct gcaccacgga
     3901 tggttccccc gggcggcgcc tggaaccaca cacgcggact gtttgcctgc taacctttag
     3961 cctgctaccc ttctccctcg cacgacgcgg gaagaacatg accggcgcac cacacatctc
     4021 ggcaccagac ctcgagggcc tcgacgacct gtcggcgcgg acgttccgcg cctttcaccg
     4081 ggcgctgcgc ctgcaccacc gcgcgctgat gcgggcgttc gcgcgccacg gcatccacca
     4141 cggccaggcg atgtgcctgc gtctcctcgc ctccgcgccg gacgtgaccc agcgcgacct
     4201 ggcgcgcgcg ctgcacgtct cgtccccgac ggtcaccagg atggtcggct cgatggagaa
     4261 ggccgggctg gtgcggcggc gccccgacgc cgcggacgcc cggctggtcc gcgtcgagct
     4321 gaccgatgcc ggccacgcgc aggagcgcgc gatgcgcgcg gccgccggcg agtacgtcga
     4381 gcacacgttc ggggcgctga ccgagaagga gcggctggag ctcgcgcgcc tcctcgagaa
     4441 actcggcgac agcatcgcgc aggtcgccgg cgaggcggac gaggcggcgc cgtgatccgc
     4501 ctcctccgca ccggcctccg cccctacctc tggtccatcg tcctcatcgt cgtcctcgtg
     4561 ttcgtccagg tcctcaccaa cctgtacctg ccgacgctca acgccgacat catcaacaac
     4621 ggggtcgtca agggcgacac cgcctacatc atcgaggtgg gcggcatcat gctcgcggtg
     4681 acgctggtct ccgcgctcgc cgccatcgcc gccgtctact tcgggtcgag gacctcgatg
     4741 gcgctcggcc gcgacacccg cggcgccctg ttccgccgcg tgcagggttt ctcgcaggcg
     4801 gaggtgaacc gcttcggcgc gccctcgctc atcacccgca cgaccaacga cgtgcagcag
     4861 gtgcagatgg tgctgctgat gatgctcaac gtggtgctga tggccccgat gatggcgatc
     4921 ggcggagtcg tcatggcgct ccgcctcaac gtcacgctct ccaccgtcat cctggtcgcc
     4981 atacccgtca tgggtgtgtt catcggcctg gtgatctcgc gcgcgacgcc gctgttcatg
     5041 tccatgcaga agaagatcga ccgcatcaac caggtcacgc gcgagttcct gagcggcgtg
     5101 cgcgtcatcc gcgcgttcga ccggacggcc tacgaggagc gccgcttcga cgtcgccaac
     5161 gaggacctga ccgacacctc gctcaaggtc gggcggctgt tcgcgctcgt cttcccggcc
     5221 gtcacgctga tcatgaactt cacgaccatc gccatcatgt ggttcggcgg caagcagatc
     5281 gccgacggca gcatgcccat cggcgacctc accgccttcc tgcagtacgt gttcatgatc
     5341 ctgttctcgg tgctcatggc cacgttcatg ttcgtgatgg tgccgcgcgc cgcggtctcg
     5401 gcgcagcgca tcatggaggt gctcgacacc gagccgggca tcgccgaccc ggagaggccg
     5461 ctgccggacg tcccggaggc cggggtggtc gagttccggg acgtggagtt ccgctacccc
     5521 ggcgccgagg agccggtgct gagcggcatc agcttcacgg cgcggcccgg ggagacgacg
     5581 gcgatcgtgg gcagcaccgg cagcggcaag accacgctga tcagcctcat cccgcgcttc
     5641 tacgaggtca cgagcggcgc ggtgctggtg gacggcgcgg acgtgcgcga actctcgcag
     5701 caggacctgt ggcgcaggat cggcctggtg ccgcagaagt cgttcctctt caacggcacg
     5761 gtggcggaga acctgcgcgt cggggacgaa caggctgccg acgagcgcct ctggcactac
     5821 ctcgggatcg cccaggcgcg cgacttcgtc gaggagatgc cggagcagct cgagacgcac
     5881 atcgcccagg gcggcgccag cgtctccggc ggccagcgcc agcgcctcgc cgtcgcccgg
     5941 gcgctggcca agcaggcgaa gatccagatc tacgacgaca gcttctccgc gctcgactcc
     6001 agcaccgacg ccaggctgcg cgcggctctg caccggcaca ccgcggaggc caccgtcatc
     6061 atcgtcgccc agcgcgtcgg gaccatcatg cacgccgacc agatcatcgt gctcgacgac
     6121 gacggcacca tcgccggcat cggcacgcac ggggagctgc tcgagcagag cgagacctat
     6181 cgcgagatcg tgacctccca gctgggcgag gagggggtcg catgagcacg ccacgcgaca
     6241 ggtcgtcgcc gccttccggg ccgcaggccg gcgtccccaa gcggccccgc ggcccgcagg
     6301 gtcccggccc cggcggaccg ggcggccacg ccctcgtgca gccgaccgag agggccaagg
     6361 acttcaaggg caccatgcgc cgcctggccg gagagctgcg gccggagcgc acgctgctca
     6421 ttctcgtcgt cctgctcgcc gtcgcgagcg tggcgttcgc ggtggtcggg cccaagatcc
     6481 tcggcaccgc gaccaacatc ctcttcgagg gcgtcgtcag ccggcagatc ccgcccggca
     6541 ccaccaagga ggaggccgtc gccggcctgc gcgcccaggg ccaggaccgc atcgccgaca
     6601 tgatcggcag catgacgctg gaccccggcg agggcatcga cttcgacgcc ctcgcccgca
     6661 tcctggccct gctcgccggc gtgtacctgg tgagcgcgct gttccagtgg ctgcagtcgt
     6721 acctgatggc gggcatcgcc cagcgcgcca tctaccggct gcggcggcag gcggacgaga
     6781 agctggcgcg cctgccgctc aagtacttcg acgaccatcc ccgcggcgac gtgctcagcc
     6841 gcatgaccaa cgacatcgac aacatctcgc agtcgctgca gcagagcctg acgcagatca
     6901 tcacctcggc cctgaccatc atcggcgtgc tcatcatgat gctcaccatc agctggctgc
     6961 tcgcgctgat ctccgtgctg gtggtgccgg tctcggtgct gatcacggtg accatcgcca
     7021 agcgctcgca gaagcagttc gccaggcagt gggagaccac cggcgacctc aacggccacg
     7081 tggaggagat gttcaccggc cacaacgtgg tcaaggtgtt cggccgccag gagaaggcga
     7141 tcgccacgtt cgaggagcag aacgagcagc tctaccaggc cagcttcaag gcgcagttca
     7201 tctccgggat catcatgccg gtgatgaact ccgtgatgaa cctcaactac gtggccatcg
     7261 ccgtcgtcgg cggcctgatg gtggccaccg gccgcatctc gctcggcgac gtgcaggcgt
     7321 tcatccagta ctcgcgtcag ttcaccatgc cgatcacgca ggtggccggc atcatgaacg
     7381 tgctgcagag cacggccgcg tcgtcggagc gcgtcttcga gctgctcgac gagccggagg
     7441 agacgcccga cccggccgag tgcgtcgtct tcgagcacac ccgcggccac atcgcgctcg
     7501 agggcgtgtc gttccgctac gtgcccgaca cgcccctcat cacggacctc acgctcgacg
     7561 tcgcgccggg tgagaccgtg gccatcgtgg gtcccaccgg cgccggcaag accacgctcg
     7621 tcaacctgct gctgcggttc tacgacatcg acgagggccg catcctcgtg gacggcgtgg
     7681 agacgcgcga catcagccgc agcgacctgc gccgcctgtt cggcatggtg ctgcaggaca
     7741 cctggctgtt cagcggcacc atccgtgaca acatcgccta cggccgggag ggggccaccg
     7801 aggaggagat ccgcgcggcg gccaaggccg cccgcgtgga ccacttcgtc cgcgccctgc
     7861 cggacggcta cgacaccgtg ctcgacgacg acgccaccaa catctcgcag ggcgagcggc
     7921 agttgctcac catcgcccgt gcgttcctcg ccgacccgga catcctcatc ctcgacgagg
     7981 cgaccagctc cgtggacacg cgcaccgagg tcctcatcca gcaggcgatg gcggagctga
     8041 tgcaggaccg caccagcttc gtcatcgccc accgcctctc caccatccgc ggcgccgaca
     8101 tcatcctggt gatggacagg ggcgcgatca tcgagcaggg cagccacgcg gagctcatgg
     8161 ccgcgcgcgg cttctaccac gacctctacg ccagccagtt cgaggaggcg ctggacgagg
     8221 cgtcctgagt ggacggggca tccggcccgg tcgccgggcg cggcgacgcg gtgactactg
     8281 ctcgagcagc gtccggtcga agaagccggt gaccagccgc tcgtactctt ccggctgggt
     8341 ctggatgccc cgcgtgtgcc ccgagccggg cacctcccag atggccttgg gggggaaggc
     8401 ggcgtcgtag tacaccgggt tcaccgcttt ctcgacctcc tggccttcct cgccgtagat
     8461 gaagtgcacg gcccgcggcg agatgagcaa cgacgcgttc ttgagcgaca tcgggacctc
     8521 ctcgccgctg agcacccaga ccgagacggt ctggacgagg tcctgcgggt actgcagggc
     8581 gagctcgagg gcgttcggtc cgcgacgcag cagggactcg cggaccgagc ggatgccggc
     8641 gccctcactc accacggcct tgaggccgga gttgccggcg gcggcctcga gcatctgctc
     8701 gccgcccacc gagaggccga gaccgccgat gcgccgccgc tgcacgtccg ggcggcggcg
     8761 cagccacgcc acggcggcgt cgatgtccgg ggtcgacccc cagccgtacg cgttggggtc
     8821 gccctcgctc tcaccgtagc cgcgcgggtc gaccaacagg acgccgtagc cgtgcttcac
     8881 gagcatgcgc gcctgctcgg tggaccaggc gcgggggaag gtgacgatcg cggcgcggtt
     8941 ctgcgacggc acgtaccagg cgccgagctc gaggccgtcg cgcgtcacca gcgtgacgtc
     9001 ctcgtacggc ctgcccaggt cggcggccgg cacgggcacg ttgggccgct cggtggcgac
     9061 gatcgccagg ctcaccggga ggacgatcca gtacaccacg aacagcgccg cgacggcgat
     9121 gagcgcgcgg cgcagcaccg tccaccacag cggtccgccg cgcttgcgcg acgtccacgc
     9181 cagccacacc cccaggacga ccagcaccgc gccggcggcg agcgggagca ggccgcagag
     9241 cgcggaggcg ctgactccct cgacgcccag ccggcggacg tgcacgcccc cggtcaccag
     9301 gccgatcacg ccgaagacga gcatcagtga cgcgcgcagg cccgccggca gacgagggta
     9361 gagctcggcg ctgagcccga ggatggccgc cgggatcacc accgcgagga cgtggtctgc
     9421 gcggtccacg cccgggcgca cgaagacgaa gtacacgagc acgaggtaga cgagcacggc
     9481 gatcacgcag gccacgaaca cccggcgctc ctgagggccg ggccggcgcg cgggcgtcgc
     9541 ggggctgccg tgcagagccg cgccgttgcc gccggccggc tcggccgcgg ccgcctccgc
     9601 gatcggcgcg tctgggccgg tcgtgctcac gtggacccct ccggggtgct cgccagaatg
     9661 actcccctag cctaccggag cggccgggcg cggcgcatcc ggcgcggtgg agtcggcggc
     9721 ggtccgctcg ctcgcaggcg gcggctcgcg caggcgcgag gcggcgacgg cggcgatcag
     9781 cagcagcccc gaggcgagca gcagcgacgc cgtgaacgag ccgtcctccg agcgcagggc
     9841 cgacatcacg aagacgaaca ccacggcgac ctgaccgaac agctggatga gcccattgga
     9901 cgtcccctcg ggggtcggcc aggtgacctc cgccgcgtac tgcatgccga cggggagcgc
     9961 cggcacgagg aagaacccca gcacgagcga cgccgcgatg agcggccaga ggctcgagaa
    10021 gagcgcgacg ccgaggagcc cggggatggc gaaggcgaag cacgccacga ggtagggcac
    10081 gcgccggccg tggcggtcgg acagcgccgg gatcaccacg gcgcccagca ggccgccgac
    10141 gagcatggcc gcgccgatga cgccggcgtc ggcctcactg aagccgcgcg ggtgcacgat
    10201 ctgctcgacc caggtcgtca cgccgttgaa cacccccatc ccgacgaaga cgagaccgag
    10261 gtagacgagg aagggcgtga ccgtcagcgc gtgccgcagg ccgtcgagca tcagcgcccg
    10321 ctcctccatc ccgggcgggc acggcggcgt cgcggggcgc tcgcgcgcga gcgccaggaa
    10381 cgccgccgcc gagacagccc cgacgacccc gaagagcagc tggaccgagg cgaccgaccg
    10441 gctctcgatg agcagcggcg tgagcgcgac gccgatgccc gtccccacca ggttcgccag
    10501 ggtgatgagc ccgacggcca gggcgcgctg ccgggccggg aaccagtgcg atcccatcgt
    10561 cgtccaggcg ttgaggagga agggctgcgc gacggcgatg ccgatggtcg ccagcatggc
    10621 gagcgtgaag tcggcgcctg cgaggccgcg caggacgccg aagaccgcca tgagcacgac
    10681 gcccagcccg acggccacgc gcaggccgcg ggcgtcgatg agccaggccg ccggcagcga
    10741 cagaggcacg aaggcgatca tgaacaccat cgagaaccag ccgatggtgg actcggacac
    10801 gccgtagtaa gcggccgcgc tggtcgtgat gggagcgtag gagatccaga ggatctggat
    10861 cgccaggttg gcgaacatga acgcgccgag gaccacccag cggtagccgt acacgcggta
    10921 ggccgcgccg gcagtcgccg cgcccgcgtc cccagccggc tccccgccca tcgcgctcat
    10981 ctcgcctgcc gccccccgcg gttgatgcgc cggtagagcg ggcgcatgcg cctgtggacc
    11041 tcgagaaagg tccggtagcg cgcatcgtac accccgcgcg ccgcgggcga cggctcgaac
    11101 tctccgtcga actcgaccag ccgcggcacg tccgagaagg cgatctcgcc gagcccggcg
    11161 gcggcgatcc acgccgctcc ccgggcgttc gcctggatgg ggtcgcggac ccggcggacg
    11221 gtgacatcga ggacgtccgc gacgatctgg caccacacgt ccgactggcc gccgccgccg
    11281 accaggttga cgacatcgac ggggcggccg aggaacttgc gcaccggctt cagcagccag
    11341 cgtgtgttca gggccacacc ctcgaagaag gcgcgcacga tgtcctcgcg cgtgttctcg
    11401 agcgagaggt tgacgagacc ggcgcgcagc gtacggtcgt cgaccggggc gcgctcgccc
    11461 cagatccacg gcgtgtagag cacgccgtta ctgccgggcg gcacgcgcgc cgcgatctgg
    11521 tcgaagacct tgaacacgtc cggctgctcc gcctcggcga gcagttcgtc ctcgtggtag
    11581 agcacgttgt cacgcaacca ggtgaggttg ccccccgcgg tggcctgcag cgccgtgagc
    11641 agccagcggc cgggcagcgc gcacggcacc gacgcgaggc cggcgatcac atcggtcttc
    11701 ttgaacggca cgtgtgcggc gagccaggac gacgtgccca ggtacaggtg cacggcgaag
    11761 tcgccggtgg cgccggcgcc gaccgccgcc gccgtggtgt cgatggcgcc ggcgacgacc
    11821 tgcgtctccg gcgccagacc cagctcctcc gccgcgtccg gagtgagcgg accgagcacc
    11881 tcggtgcact tgacgatctc gggcagcttg cctgcgtcga cgccgcagtc acgcacgagc
    11941 gcggcgtcgt acctcacgcc gccggcgtcg cggttgtcgg tcacccagct ggtcatgatc
    12001 gagtcgtagc tcgccgccat gcggccggtg agcctgaggt tcaggtagtc cggcacgccg
    12061 aggaacttgt gcgtgcgcgc atagacttcc ggcagctcgt cgcggacgta gagcatgtgc
    12121 gccgccgggt ccttgccggt cggcgagggc atgccgccgg tgagcttcac ccagcggcgg
    12181 atgcgtggca ggctgaggcc gtgcatgctg agcggcccgc cgaactgccg ccgcaggtgc
    12241 ggcgcgccgc gcatgtccat ccagaggatg cagttcgtga gcggtgcgcc ggtctcgtcc
    12301 accggcaccg tgccctcgcc ctgggcgctg cagcagacgg cggcgatccg ggcggccgga
    12361 ccgtcgtggc gggcgagcag tcgccgcgtc gcggcgacca cggcccgcca ccagtcctcc
    12421 ggccgctgct ccgcgccgcc gcccggcagc acgatgagct cgacgggctc gaacgcccac
    12481 gcgaccacgc gcccgtcgat cccgatcagc gccaccttgg cgccggacgt gccgagatcg
    12541 acggcgagga cgtggcggga gccgctcgag gtgccgacca ttgcggcagc atagcaccgg
    12601 cacccggcct cggagacgcc tgctacaggt acccgcgccg gcggagcacc gtgagcaggc
    12661 cgccgctcac ggcgaccatc acgacgatcg cgacgctgaa gaaccactgg ttcatgaaga
    12721 ggtcggggag catgttcatg ccgaagatgc ccgcgatcag cgaggccggc agcagcagcg
    12781 ccgagatcac cgtcaggacc ttcatcacgt cgttggtgcg ctgcgccgtg cgcgtcatat
    12841 acaggtcgaa cgcgccgacg atgagctccc gggcgctctg cgcggcctgc acggcctgct
    12901 cgacacgctc gccgagcagg gcgaagtcgc cggccgcgtc gctgcccgcg acgacgtccg
    12961 agctggcgtg cgcgagggtg ccgtagacgt agcgatgcgg gctgaggccg ctgcgcagat
    13021 agctcacccg gcgacggcgg gcgacgagct catcgagcga acgcctcgcc agctcatcgc
    13081 ccaggatgtg ttcctcgatg ctgtcggtcg cgcgctgctc gcgttccatg gccaggaaga
    13141 actcgttgag ctcccactcg agcagtgagg cgaggaagtt gggcgcgtcc agctccccca
    13201 gggagctgtc cgagtggacg tgctcgtaga acgcgtcgag gaactcgatc tcctcctggt
    13261 gcgcggtcac cagccagcga gcagcggcga tgcaggtgag gggagtgagc tcggcgtcgt
    13321 cctcctgaga cggcagcgtg gggatggtga acgagaagga gccgccgcgc tccctgaccg
    13381 gcaggcgatc ctcctcttcg aactcaccct cgtcgatctc gacgagggtg cggagttgct
    13441 ccaccgcggc cggcgaggcg aggtcgacgt cgacccacag gagttgatcc ttggtgagcc
    13501 ggccggcgat cccctcgctg agctcgacct ccttgtctct ggccttggcg tcgtagagaa
    13561 aggcgcgcaa gcccttggcc tgcttcgtcg tggtgttccg gccgcgatcc tgcgcgccgc
    13621 gtccggaggc ttgcgtcccg gtcatgctca cctcccgccc tcgtggagcg ttcgttccca
    13681 gcgggagggc ggctcaagcc gtctgccgtg gtcaaggctc gcgccggcgc agcccgggga
    13741 accgcgcgaa gtcgcggtcg gcggtcacga ggaccgcgcc ggtctcgagg gcaagggccg
    13801 cgagataggc gtccgtcacg agatccccgt gagcgtccgc ctcgcggcac aggccctcga
    13861 acagcgcgag gtggtcgcgg cccgggttga gcagcagggc gttgggatgc gtcatcaggg
    13921 agttgacgaa agcgaaggcc tgatcgtgcg tgtcgggcgg gcggaacacg cgcggatggg
    13981 tgaggatgcg taccagcccg atcagtaccg gaggacagat cccgaacgat tcgtctccgg
    14041 cgtacaccgc gagtagccag tcgcggcagc gcgggtgcat cgggctgtcc gcgcgatgcg
    14101 cgctgatgag gacattgaga tcgggcagaa acaccgcggc gtcagcggtg gcccgcgtcc
    14161 ggaccgtaca ggtactcggc catgccggat ggactcgtca ggtcgacgcc gggggcgagt
    14221 ccccggccac cgacgatggg gaactccacc ggcttggttg gcgcgacgtc gcgcccgcgg
    14281 ctcaggtgtt cgcgcagtgc gtcctcgatg agccggctga gtgtcagatg ggtctccgct
    14341 gagcggcgct tgaactccgc gagcacctgc gaatcgatgg tcaacgtcgt gcgcatgatg
    14401 gtctccatag ccggagtatg cagccaagac atcatagcat caatacatca accaggtgac
    14461 gttggaccgc ctccttccgc cccgctcagc gcaacgcgag cagcccggac acggtcacga
    14521 agctgaagcc gcgtgcctgg agcgccgcga ggatcgccgg cagcgcccgg cacgtcgcgg
    14581 cgttgccctc cgcccacgga tgcaacagga cgatggacgc gggccgcacg ttgtccacca
    14641 cgtaccgcgt catggcttgc gcgtcgtccc ggatgccgtc cagagagtcc ggctccaggt
    14701 cccacatcac ggtgttgcgg tcgcgggccc gcagctccca cggcagcgag agcagcttct
    14761 tgccgtgggg cggccgcacg ctgatgggcc ccctgcatcc ggcggcgcgg atcaccgcgt
    14821 cggcggccgt ggcggtggac acgaacacga gcctgcggtg gttgaaggtg tggttgccga
    14881 tctcctggcg cggtgacgag gctgcgcagc gcgacggggt gatcggacgc ggccgcgccg
    14941 atcacgtaga acgtggcgcg ggcttggcag cgccggaggt ccgtgaggtc atcaggcggt
    15001 acgatcccgc accgacgacc aggagcgcga cgaccgcagc ggcggcgatg gcgtcgaggc
    15061 tcaccccgcc gtcagacgcc ctcgacgctc gcgagcacgc gctcgaagcg ctcgagcgcc
    15121 tcgtcgatca ccgcgtcgct gtcggccatc gacgtgtaca tgcgggagcc ggcgagagtg
    15181 accaggccgt tcgcggtgaa ggccgcgccc atctcctcca tcatgtgctt gcgcggcttc
    15241 atctgcttcg cgaagcgcag cgggtgcttg aggtcgagga gcatcacgcc cgaggtctcg
    15301 aggtggacga tcgagccctg gttgtaggcg acgaacggca gggagtactt ctcgatgagg
    15361 aggcgcaggc cggccgtgag ccggtcgccg gccattcctg ccagcaccgg cgcgccggtc
    15421 cgctccatct cggtgatggc gaagtaccca gcggcgcagc tgagcgggtt ggcgctcagg
    15481 gtgccgccga cgtaggcgcg ctcgccggtc ttgccgccga tgccgccggc gaagctcgcc
    15541 atcacgtcgc ggcggccgcc gacgccgccg gccatggggt agccgcccgt gatgcacttg
    15601 ccgaagatgg tgaggtcggg gcgcacgccg aagtacccct gcgccccgcc cagcccgagg
    15661 cggaagccgg tgacgacctc gtcgaagatg agcagcgcgc cgaactcgtc gcacagctcg
    15721 cgcacggcgg cgttgaagcc gaacggcacc ggccgcgtgc cgctctccgg gccgaccggc
    15781 tcgaggatga ccgccgccgt gccgcccatc gactggttct gccgcagctt cctgcgcagc
    15841 gcatcgaggt cgttggggaa cacctcgcgc gtgcgcgccg tcgccccgaa cgggatgccc
    15901 ttggcctcga accgccaggt gcctggcacg tgcaacccgt acaccatctg gtcgctccag
    15961 ccgtggtacg cgcccccgac cttgaccacc cacttcttgc cggtgaaggc gcgggcggcg
    16021 cggacggccg ccatgacgct ctccgtgccg gagccgagca tgcggaacat ctcgca
//
LOCUS       DASUMX010000016        11179 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_292271, whole genome shotgun sequence.
ACCESSION   DASUMX010000016 DASUMX010000000
VERSION     DASUMX010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 11179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11179
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292271"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1173)
                     /locus_tag="VFZ86_00780"
     CDS             complement(<1..1173)
                     /locus_tag="VFZ86_00780"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX5640851.1"
                     /translation="MGFAAAFALLVSFVDRTSVLQVAAVLLALFAVVAVTFDLWVYRP
                     VNDLIRRSRTRLGGNYARNDPFYRDEVRELGHLVGTLIAVFLAAEDKEWVSQSIKDDL
                     IRVQALNRQLMDVGVLGREMNAALPYLETVERVLSRSRTFLHADFGALLLLDAEARSF
                     TLEGAQGVLTPTLSAECCAYTPDCPVRQAISSGRITRAEGHVCTLFPQTMTHQLVIPV
                     AIENVGDMALLTTSTSGEYTQLLTGDILDALQNHVQSALTNAHKYDAIRRQVVTDHLT
                     GLYNRRFFMNRAGEEIERSLRHQAPLSVLMIDIDHFKQFNDTYGHATGDRVLQTVARA
                     MKDALRKPDICSRHGGEEFAVLLPNTPGDSAYHVAERVRKTLSGTRYTGLGLPPDVN"
     gene            complement(1390..1575)
                     /locus_tag="VFZ86_00785"
     CDS             complement(1390..1575)
                     /locus_tag="VFZ86_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5640852.1"
                     /translation="MKDPGTAPQPPLICVADVDTRLSCSCYCAVCPYYLRDRRIGERR
                     TVHRPTRDRRILNRFKG"
     gene            complement(1665..2033)
                     /locus_tag="VFZ86_00790"
     CDS             complement(1665..2033)
                     /locus_tag="VFZ86_00790"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5640853.1"
                     /translation="MANAILVADDDPDILSIVSMSLETQGYTVHKATNGREAVDLAHQ
                     HHPDLILMDMMMPVVSGYEAVAELKADADTRDIVIVGLSAKAMATDMERATDVGIDGY
                     ITKPFRIAQVLSVVESYLDA"
     gene            complement(2049..3887)
                     /locus_tag="VFZ86_00795"
     CDS             complement(2049..3887)
                     /locus_tag="VFZ86_00795"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5640854.1"
                     /translation="MDLRKQLHDAMWMRVLFTIALLTGVLWVQIDDHGVAAAELLIGL
                     AIVALVNLPMYIVERRLPTRTTAAIIVGSDLVLVTAGVIFAGGALSPEGVFYVWPIVF
                     SAVFLAAWAPYAAAAVASVAYVTVWELQHVGWLDVPAVVAQVQVPANWMLITVSLHVA
                     AFMLVALLSGRLARALIGSSTQLSRAKADTEAQLRRMRATNEQLRVMSESSRLFLRHQ
                     DAAGLVPEALDQIASATGVRSGFALILNHNSHDFEERALAGDVTADAVRGYKELGIVT
                     VAQAGAERYETVTDPKVRQLLKAMEKDGFRGFLVAPLQAKHETLGVICLLHREDEAVP
                     DAAVATLRALCDQVALVLRNIQFNEELARKNRDLTHLDELKSDFMATMSHELRTPLTS
                     IIGYSDMLLSGMTGELNEKQSAFVDSILKGGEALLGLINDILDLTKIEAGRLELNREA
                     VDLRAALLGVLPVVKPRAQDKRIRISTFLPTDLPLVWADPAKLNQILLNLITNGIKYT
                     HENGSVSIEARPLEGLVEIWVTDTGIGVAKEDQDKVFQRFTQIDSSATRSQGGTGLGL
                     AIVRELVELHGGTIRLQSKLAKGSSFIFTMPISTTPADPLAAGKIS"
     gene            4032..5390
                     /locus_tag="VFZ86_00800"
     CDS             4032..5390
                     /locus_tag="VFZ86_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5640855.1"
                     /translation="MCGLEPGPAVALASGALLAAFADERAVRPVLLGADLPLWRLLYA
                     ASARAPRILDPRLVGDAVAGELYDLWASGSDLTTVVAVEPALDRWEGVKGSRAVDVAA
                     ALDAPLVLVVDARERGSTAAAAACGARLLAGGVEFAGAIVVGGDDRGAAAELRGILEE
                     TAKLPVLGWIPPQLSEQFARQYGGGQSGLRQIGPQPVKGGETALCREAGGFLDREGLA
                     AAAARRGYLPATARRLLSPLPAATGLTLAVAWGPPLEPFALDAVDLFKALGLTLEPLR
                     IGSDKALPEGAAGLLLAGLLDEEQLPAFAGNEALKSGLVAAVAGGLPVLALGGGALLL
                     LRRLADSRGRTHELAGILPAEAELLEWYERPRYVRAAAARENPYAEGEVTLYELFDLE
                     FLTLQQEAFAWRVGDAGAEGQVEGFVHGRCLATTLVPSLPATPGLAESFVAAMRLAAP
                     AG"
     gene            5387..7066
                     /locus_tag="VFZ86_00805"
     CDS             5387..7066
                     /locus_tag="VFZ86_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5640856.1"
                     /translation="MSLPALESWLRTRRGLLTLWLVTTAAVALVTLLMELGGVPGWDD
                     AAHAYKVHLLRDGDSVFWDTYWYGGGYGAITYGFVFYWLAQYVSGALIVILVAGSIPV
                     SFYLYVRDLWGIDDVWPAWGFALVMTLYLAHGQDPFVLALALTLAGLALLARRRPLWA
                     ALPVALGIFANPMGLVVVAPFVLTDLIVRPAARRRYALFAAALAPAVLVRFAIGLGFS
                     EPGAYLNETSQLMVFLGFALVGVALAGVNAVHPRRPFIVLFLVYAGLCVGSFITPGSP
                     IGNNSGRFFMVFGLPLLLLLRHTRLRRPFRYGDLAIIPIVLFALLQFGTATSHFLNTV
                     ERPQTTRAFFAPALDAAGDLYDPNYRFHVVALRRHWEALYFPEAGYPITRGWYRQADA
                     IHNGLFYTPYDAAEYAAWLRSMGVEYIFSPVVGPLDPWSRREARLLESSRAFVFVEQA
                     GAWRIYRLLDAEPILVPDPAVPAAGGAPGEGTISFFGHERIMFSVTRPGTYWLKVTSS
                     PYWVLEGGPGTVVPRTDRFMDLRLRRAGAYTLRFVVTPARTLDVIGGRFGL"
     gene            7110..8207
                     /locus_tag="VFZ86_00810"
     CDS             7110..8207
                     /locus_tag="VFZ86_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate phosphoribosyl transferase"
                     /protein_id="HEX5640857.1"
                     /translation="MPRSRLAPEVFALPVDRLREGYYTDAYFNGAKRVLETKDLHPRV
                     TMQVFQKTDGAVLGGMDEALAILRLCTGRYVAEGGERVWVDGWDHLDVRALHDGDELS
                     GWETVLTVEGDYALFAHLETVYLGVLSRRTLISTNVRRVVDAARGKPILFFPARFDHY
                     RVQTGDGYAAHIAGAIGVSTDAQASWWGGKGIGTVPHALIAAYGGDTVAAAAAFADVH
                     YPEINVTALVDWDNDCVATSLACARALGERLWGVRLDTSETMVDRSLWDDMGAFRPNG
                     VAPELVRAVRRALDDEGFQHVNIVVSGGFDAERIRRFEAAGVPADAYGVGSSLLRGNT
                     DFTADVVLLEGRPCAKVGRRHSPNPRLERVT"
     gene            8234..8785
                     /locus_tag="VFZ86_00815"
     CDS             8234..8785
                     /locus_tag="VFZ86_00815"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HEX5640858.1"
                     /translation="MAGDTHKRRAFVVVDMLVCFCRRGALYSPRYDRMVPELVRRLAA
                     EEAEGTALVFLIDTHVRDDPEFAMFPEHCVEGSGEDEVIPELRAFAERGTVVRKRTFS
                     GFHETELEAVLERLAPDVVEVAGVCTDICVLHTVAGLRARGYEVVVRKDLVDTYDAPG
                     HDAEEFNRFALAHVRDVLGARVE"
     gene            complement(8798..9454)
                     /locus_tag="VFZ86_00820"
     CDS             complement(8798..9454)
                     /locus_tag="VFZ86_00820"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5640859.1"
                     /translation="MRRSLALIAAAIAVAAAAAVIVVGMSSGDAEPTGAPEPAAGGGL
                     PSGHPTVAVDQDEPGMQGADDDAGEDGDAQTGEDIAHLEDRRRQDPGDVAVLLELGNA
                     YFMAQRLQQAERAYTQALALAPDDATAQVGLAMVRHAQGDSKQAESMLRAVIEEHPDD
                     QDAHYSLAIVCFSSGRVDEAKEQWQTAAGIDPRSTTGRRSQSFVDLLEDQQSGAPAAN
                     "
     gene            9693..10115
                     /locus_tag="VFZ86_00825"
     CDS             9693..10115
                     /locus_tag="VFZ86_00825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX5640860.1"
                     /translation="MGLQLTRGAEYAIRAMSYLARFPEGHVASLHEIGEAQDIPESFL
                     AKILQSLVRAGLTVSQRGAHGGFALAHPASEITMQQVIEAVDGPISVNQCVLSPEDCA
                     RSASCTVHEAWLRAQGQLMDVLGSITLESLAPRPAAVG"
     gene            complement(10159..11133)
                     /gene="galE"
                     /locus_tag="VFZ86_00830"
     CDS             complement(10159..11133)
                     /gene="galE"
                     /locus_tag="VFZ86_00830"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019006222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEX5640861.1"
                     /translation="MRVLVTGGAGYIGSVIVARLAARGHDVVVYDDLSRGHAAAVPEG
                     VPLVRGDIRDTAAVRAALAEGGCQAVVHMAALAEVAESVAEPERYFSVNRDGTAAVIA
                     AAAAAGAGRLVFSSTAAVYGAPERTPIGEGDTLAPANPYGETKLAGERLLEQARSGGE
                     FAATALRYFNACGADGACGEDHDPESHLIPLALRAARDGSAIRVFGDDYPTPDGTCVR
                     DYVHVADLADAHIAALEALPGVQGSFNLGTGTGDSVQAVLDAVETVTGLTLARETAPR
                     RAGDPPVLVASNRRAAEHLGWRPRRSLTDAVADAWAWMREHPDGYAER"
BASE COUNT         1416 a         3986 c         4069 g         1708 t
ORIGIN      
        1 gttcacgtcg ggcgggagcc cgagcccggt gtagcgcgtg ccgctgaggg tcttgcgcac
       61 gcgctccgcc acgtggtacg cgctgtcgcc cggcgtgttg ggcagcagca cggcgaactc
      121 ctcgccgccg tgacgcgagc agatgtccgg cttgcgcagt gcgtccttca tcgcccgcgc
      181 gaccgtctgc agcacgcggt cgccggtcgc gtgcccgtag gtgtcgttga actgcttgaa
      241 gtggtcgatg tcgatcatga gcacgctgag gggcgcctgg tgccgcaggc tgcgctcgat
      301 ctcctcgccg gcgcggttca tgaagaagcg ccggttgtag aggccggtga ggtggtcggt
      361 gaccacctgg cggcggatgg cgtcgtactt gtgggcgttg gtcagcgcgc tctggacgtg
      421 gttctggagc gcgtccagga tgtccccggt cagcagctgc gtgtactccc cgctcgtgga
      481 ggtggtcagg agcgccatgt ccccgacgtt ctcgatcgcg accgggatca ccagctggtg
      541 ggtcatggtc tgcgggaaca gcgtgcagac gtgaccctcg gctctcgtga tgcgccccga
      601 ggagatcgcc tggcgcacgg ggcagtcggg cgtgtacgcg cagcactcgg cggagagcgt
      661 cggcgtcagc acgccctggg cgccctcgag cgtgaaggag cgtgcctcgg cgtcgagcag
      721 gagcagcgcg ccgaagtcgg cgtgcaggaa cgtgcggctg cgggagagca cgcgctccac
      781 cgtctcgagg tacgggagcg cggcgttcat ctcgcgcccc agcacgccga cgtccatgag
      841 ctgccggttg agcgcctgca cgcggatcag gtcgtccttg atgctctgcg agacccactc
      901 cttgtcctcc gcggcgagga acaccgcgat gagcgtgccg acgaggtggc cgagctcgcg
      961 gacctcgtcg cggtagaagg ggtcgttgcg cgcgtagttg ccgccgagcc gcgtgcgcga
     1021 ccgacggatc aggtcgttga cgggtcggta cacccagagg tcgaaggtca cggcgacgac
     1081 ggcgaacagc gccagcagca cggcggcgac ctgaaggacc gatgtgcggt cgacgaagga
     1141 gacgaggagg gcgaacgcgg ccgcgaaccc cacggcgacg accgccgtgg tcttcagctc
     1201 aagactgatg cctcgcttgt ggccgaggct tccgcctcgt tcctgctccg gtgaccggcc
     1261 cgtgactggc ctcgattcgg gggcttctcg tgcctggata cggatatcgg cagaagcccc
     1321 cgcggccatg agagagggcg ctctcgcacg cgagccggcc gccgggacgg ccggccgcgg
     1381 ccggccgggt cagcccttga agcggttgag gatgcgccgg tcgcgggtcg ggcggtggac
     1441 cgtccggcgc tcgccgatgc ggcggtcacg caggtagtac gggcagacgg cgcagtagca
     1501 gctgcagctc aggcgggtgt cgacgtcggc gacgcagatg agcgggggct ggggggctgt
     1561 gcctggatcc ttcacgggtg catcacgtga agtgtacggc gtggacgggc gcgctcctgc
     1621 ccggcgtcgt cagccctgac gacgggcctg ccggcgcgcc ctgctcatgc gtccaggtag
     1681 ctctccacca cggagaggac ctgggcgatg cggaacggct tggtgatgta gccgtcgatg
     1741 ccgacgtcgg tggcgcgctc catgtcggtc gccatcgcct tggcggagag gccgacgatg
     1801 acgatgtccc gtgtgtccgc gtcggccttg agctcggcga ccgcctcgta gccgctcacg
     1861 accggcatca tcatgtccat gaggatgagg tcggggtggt gctggtgggc gaggtcgacg
     1921 gcttcgcgcc cgttcgtggc cttgtggacc gtgtagccct gggtctccag agacatcgag
     1981 acgatgctga ggatgtctgg gtcgtcgtcg gcgaccagga tggcgttcgc cacgctgcgc
     2041 tcctcccctt agctgatctt gcccgccgcc agcgggtccg ccggcgtggt agatatcggc
     2101 atggtgaaga tgaagctgga gccctttgcg agcttgctct gcagccggat ggtgccgccg
     2161 tgcagttcca cgagctcacg cacgatcgcc agccccagcc cggtgcctcc ctgggagcgg
     2221 gtcgccgacg agtcgatctg cgtgaagcgc tggaagacct tgtcctggtc ctccttggcg
     2281 acgccgatac cggtgtcggt gacccagatc tcgaccagcc cctccagcgg gcgcgcctcg
     2341 atgctcacgc tgccgttctc gtgcgtgtac ttgatgccgt tggtgatgag gttgaggagg
     2401 atctggttga gcttggccgg gtcggcccac acgagtggca ggtcggtggg caggaacgtg
     2461 gagatgcgga tgcgcttgtc ctgggcgcgc ggcttgacca cggggagcac gccgaggagg
     2521 gccgcccgca gatcgaccgc ctcgcggttg agctccaggc ggcccgcctc gatcttcgtg
     2581 aggtcgagga tgtcgttgat cagcccgagc agcgcctcgc cacccttgag gatgctgtcg
     2641 acgaacgccg attgcttctc gttgagctcg ccggtcatgc cgctgagcag catgtcgctg
     2701 tagccgatga tggacgtgag cggggtgcgc agttcgtggc tcatggtcgc catgaagtcg
     2761 ctcttgagct cgtcgaggtg ggtgagatcc cggttcttgc gcgccagctc ctcgttgaac
     2821 tggatgttgc gcagcaccag cgccacctgg tcgcagaggg cgcgcagggt cgccacggcc
     2881 gcgtcgggca cggcctcgtc ctcgcggtgg agcaggcaga tcacgccgag cgtctcgtgc
     2941 ttggcctgga gcggcgcgac gagaaagccg cgaaaaccgt ccttctccat cgccttgagc
     3001 agctgccgta ccttggggtc cgtgaccgtc tcgtagcgct cggcgccggc ctgcgcgacc
     3061 gtgacgatgc cgagctcctt gtagccgcgc acggcgtccg ccgtgacgtc gccggcgagc
     3121 gcgcgctcct cgaagtcatg ggagttgtgg ttgaggatga gcgcgaaccc gctgcgcacg
     3181 cccgtcgcgc tcgcgatctg gtccagagct tcggggacga ggccggcggc gtcctggtgg
     3241 cggaggaaca ggcggctgct ctcgctcatc acgcgcagct gttcgttggt cgcccgcatg
     3301 cggcgcagct gcgcctcggt gtcggccttg gcgcgggaga gctgggtgga gctgccgatg
     3361 agcgcccgcg ccagccgccc gctgaggagc gcgaccagca tgaacgcggc gacgtggagg
     3421 ctgacggtga tcagcatcca gttggcgggg acctggacct gggcgacgac cgccggcacg
     3481 tcgagccagc cgacgtgctg gagctcccag acggtgacgt aggcgacgct ggcgacggcg
     3541 gccgcggcgt acggcgccca cgccgcgagg aacaccgcgg agaagacgat cggccagacg
     3601 tagaagacgc cctccgggct cagggcgccg ccggcgaaga tcacgccggc ggtgaccagc
     3661 acgaggtcgc tccccacgat gatcgcggcg gtcgtgcgcg tgggcagacg ccgctcgacg
     3721 atgtacatcg ggaggttgac gagcgcgacg atcgcgagtc cgatgagcag ctccgccgcc
     3781 gccacgccgt gatcgtcgat ctgcacccag aggacgccgg tgaggagcgc gatggtgaag
     3841 agcacgcgca tccacatggc gtcgtgaagc tgcttgcgca ggtccataag gtcggctcgg
     3901 tcctcctgaa ggctggtaga gtattctatc ccggcccccc ggaatccgcc ctgccggtac
     3961 gccggtccgg ggccgctacg gggagcccgg ccgaaaagga ggacaccatc acgtccgccc
     4021 ctcgcctcgt cgtgtgcggg ctggagcccg gtcccgccgt cgcgctggcc tccggcgcgc
     4081 tgctcgccgc gttcgcggac gagcgcgccg tccgccccgt cctcctcggc gccgacctgc
     4141 cgctctggcg gctgctgtac gccgcctccg cccgcgcccc ccggatcctc gacccgcggc
     4201 tcgtcggcga tgccgtcgcc ggagagctgt acgacctctg ggcctccggc agcgacctca
     4261 ccaccgtcgt cgccgtggag ccggcgctcg accgctggga gggcgtcaag gggtcgcgcg
     4321 cggtggacgt ggccgccgcg ctcgacgccc cgctcgtgct cgtcgtcgac gcccgcgagc
     4381 ggggctccac cgcggccgcc gccgcctgcg gcgcgcgcct gctcgccggc ggcgtcgagt
     4441 tcgcgggggc gatcgtcgtc ggcggcgacg acaggggtgc cgccgccgag ctgcgcggca
     4501 tcctcgagga gaccgccaag ctccccgtgc tcgggtggat accgccgcag ctgagcgagc
     4561 agttcgcgcg ccagtacggc ggcggccaga gcgggctgcg ccagatcggg ccgcagcccg
     4621 tcaagggagg cgagacggcg ctctgccggg aggccggcgg cttcctcgac cgcgaagggc
     4681 tcgccgccgc ggccgcgcgc cgcggctacc tgccggcgac cgcccgccgg ctgctctccc
     4741 cgctgcccgc ggccaccggg ctgacgctcg ccgtggcctg ggggccgccg ctcgagccgt
     4801 tcgccctcga cgccgtcgat ctgttcaagg ccctggggct cacgctcgag cccctgcgca
     4861 tcggcagcga caaggccctg ccggagggcg cggccggcct cctgctggcg gggctgctcg
     4921 acgaggaaca gctgccggcg ttcgccggca acgaggcgct caagtcgggg ctcgtcgcgg
     4981 ccgtcgccgg ggggctgccg gtgctcgcgc tcggcggcgg cgccctcctg ctcctccgcc
     5041 gcctggccga cagccgtggc cgcacccacg agctcgccgg catcctgccc gccgaggcgg
     5101 agcttctcga gtggtacgag cggccgcggt acgtgcgcgc cgccgcggcg cgcgagaacc
     5161 cctacgccga gggggaggtc accctgtacg agctgttcga cctcgagttc ctcaccctcc
     5221 agcaggaggc cttcgcctgg cgcgtcggcg atgccggcgc cgaggggcag gtcgagggct
     5281 tcgtgcacgg gcgctgcctc gcgaccacgc tcgttccctc cctgccggcg acgcccggcc
     5341 tggccgagag cttcgtcgcg gcgatgcggc tggcggcgcc tgccgggtga gcctcccggc
     5401 gctcgagtcc tggctgcgca ccaggcgcgg cctgctcacg ctctggctcg tgaccaccgc
     5461 cgcggtcgcg ctggtcacgc tgctcatgga gctcgggggc gtgcccggat gggacgacgc
     5521 cgcccacgcc tacaaggtcc acctgctccg cgacggcgac tccgtcttct gggacacgta
     5581 ctggtacggc ggcgggtacg gcgccatcac ctacggcttc gtcttctact ggctcgcgca
     5641 gtacgtctcc ggtgcgctca tcgtcatcct cgtggccggc tcgatcccgg tctcgttcta
     5701 cctctacgtg cgcgacctgt ggggcatcga cgacgtgtgg ccggcgtggg gcttcgcgct
     5761 cgtcatgacg ctctacctcg cgcacggcca ggaccccttc gtactggcgc tggcgctgac
     5821 gctggccggc ctcgcgctgc tcgcgcgccg ccggcccctg tgggcggcgc tgccggtcgc
     5881 cctcggcatc ttcgcgaacc cgatgggtct ggtggtcgtg gcgccgttcg tgctcacgga
     5941 cctcatcgtc cggccggcgg cgcggcggcg ctacgcgctg ttcgccgccg ccctcgcccc
     6001 ggccgtcctc gtgcgcttcg ccatcggcct cgggttctcg gagccgggcg cgtacctcaa
     6061 cgagacgtcg cagctcatgg tcttcctcgg cttcgccctc gtcggcgtgg cgctggccgg
     6121 cgtgaacgcc gtccacccgc gccggccctt catcgtgctc ttcctcgtgt acgcgggcct
     6181 ctgcgtcgga tcgttcatca cccccggcag cccgatcggc aacaacagcg gccgcttctt
     6241 catggtcttc gggctgccgc tccttctcct gctccgccac accaggctgc gccggccgtt
     6301 ccgctacggc gacctcgcga tcatccccat cgtgctcttc gccctcctgc agttcggcac
     6361 cgccaccagt cacttcctca acaccgtcga gcgcccgcag accacgcgcg cgttcttcgc
     6421 ccctgccctc gacgccgccg gggacctgta cgacccgaac taccgcttcc acgtggtggc
     6481 gctgcgccgc cactgggagg cgctctactt ccccgaggcc ggctacccca tcacgcgcgg
     6541 gtggtaccgc caggccgacg ccatccacaa cggcctcttc tacacgccct acgacgcagc
     6601 cgagtacgcc gcctggctgc gcagcatggg cgtggagtac atcttctccc cggtcgtggg
     6661 accgctggac ccctggagca ggcgagaggc ccggctgctc gagagctccc gcgccttcgt
     6721 cttcgtcgag caggccggcg cctggcgcat ctaccggctg ctcgacgccg agcccatcct
     6781 ggtcccggat cccgccgtgc cggcggcagg gggcgcgccg ggagagggca cgatcagctt
     6841 cttcgggcac gagcgcatca tgttcagcgt gacccggccc gggacgtact ggctcaaggt
     6901 cacctcgtcg ccgtactggg tgctcgaggg tgggcccggg acggtggtgc cgcgcaccga
     6961 ccggttcatg gacctccggc tgcgtcgcgc gggcgcttac acgctgcgct tcgtggtgac
     7021 cccggccagg acgctcgacg tcatcggcgg caggttcggc ctctagcgcc ggagcccgcc
     7081 gcggccggcc gtgggggtac aatccggcca tgccgcgctc acgtctggca ccagaggtct
     7141 tcgccctccc cgtcgacagg ctgcgggagg gctactacac ggacgcctac ttcaacggcg
     7201 ccaagcgcgt gctcgagacc aaggacctgc acccccgcgt gacgatgcag gtcttccaga
     7261 agaccgacgg cgcggtcctc ggggggatgg acgaggcgct ggccatcctg aggctgtgca
     7321 cggggcgcta cgtcgcggaa ggtggcgagc gggtctgggt ggacggctgg gaccacctcg
     7381 acgtgcgcgc gctccacgac ggcgacgagc tgtcggggtg ggagacggtg ctgacggtcg
     7441 agggcgacta cgccctcttc gcccacctgg agaccgtgta cctcggcgtg ctctcgcggc
     7501 gcacgctcat cagcaccaac gtgcgccgcg tcgtcgacgc cgcgcgcggc aagccgatcc
     7561 tcttcttccc ggcgcgcttc gaccactacc gggtgcagac cggcgacggg tacgccgccc
     7621 acatcgccgg cgccatcgga gtgagcaccg acgcgcaggc gtcctggtgg ggcggcaagg
     7681 gtatcggcac ggtgccgcac gcgctcatcg ccgcgtacgg cggcgacacc gtcgcggcgg
     7741 cggcggcgtt cgccgacgtc cactaccccg agatcaacgt cacggcgctg gtcgactggg
     7801 acaacgactg cgtcgccacc agcctcgcct gcgcgcgggc gctcggcgag cgcctctggg
     7861 gcgtccgtct cgacacgagc gagaccatgg tggaccgctc gctctgggac gacatgggcg
     7921 cgttccgccc caacggggtg gcgccggagc tcgtccgcgc cgtgcgccgc gccctcgacg
     7981 atgaggggtt ccagcacgtc aacatcgtcg tcagcggagg cttcgacgcc gagcgcatcc
     8041 ggcgcttcga ggcggccggg gtaccggcgg acgcctacgg cgtcggcagc agcctgctgc
     8101 gcggcaacac cgacttcacc gccgacgtcg tgctgctgga gggccggccc tgcgccaagg
     8161 tcggccggcg ccacagcccg aacccgcgcc tcgagcgcgt gacctagagc ccctcgccgg
     8221 gtatcgcacc ggtatggccg gggacacgca caagcggcgg gcgttcgtcg tcgtcgacat
     8281 gctggtctgc ttctgtcggc gcggcgctct ctacagcccg cggtacgaca ggatggtgcc
     8341 ggagctggtc cgccgcctgg ccgccgagga ggcggagggc acggcgctcg tcttcctcat
     8401 cgacacgcac gtgcgcgacg accccgagtt cgccatgttc ccggagcact gtgtggaggg
     8461 gtcgggggag gacgaggtga tccccgagct cagggcgttc gccgagcgcg gcaccgtggt
     8521 gcgcaagcgc accttctcgg ggttccacga gacggagctc gaggcggtgc tcgagcgcct
     8581 ggcgcccgac gtcgtcgagg tcgccggcgt ctgcaccgac atctgcgtgc tgcacacggt
     8641 cgccgggctg cgcgcgcgcg gctacgaggt ggtcgtgcgc aaggacctcg tcgataccta
     8701 cgacgccccg gggcacgacg cggaggagtt caaccgcttc gcgctcgccc acgtccgcga
     8761 cgtgctgggc gcgcgcgtcg agtagcgggc cggccggtca gttcgcggcg ggcgcgccgg
     8821 actgctggtc ctcgagcagg tcgacgaagc tctgcgagcg cctgcccgtg gtcgatcgcg
     8881 ggtcgatgcc ggcggcggtc tgccactgct ccttggcctc gtcgacgcgc ccgctggaga
     8941 agcagacgat ggcgagcgag tagtgggcgt cctggtcgtc cgggtgttcc tcgatcaccg
     9001 cacgcagcat ggactcggcc tgcttggagt cgccctgcgc gtgccggacc atggccaggc
     9061 ccacctgcgc cgtggcgtcg tccggcgcca gcgcgagcgc ctgtgtgtac gcccgctcgg
     9121 cctgctgcag gcgctgggcc atgaagtagg cgtttccgag ctcgagcagg acggcgacgt
     9181 cgcccggatc ctgccggcgg cggtcctcga ggtgggcgat gtcttcgccg gtctgcgcat
     9241 ccccgtcctc gccggcgtcg tcgtccgcgc cctgcatccc cggctcgtcc tggtcgacgg
     9301 ccacggtcgg gtgtccggag gggaggccgc cgcctgccgc cggctcgggc gcgccagtgg
     9361 gctcggcgtc acctgagctc atgccgacga cgatcaccgc ggccgcggcg gccacggcga
     9421 ttgcggcggc gatgagggcc agggatcgtc tcacacttgt ccttccgtgg gggacgcgct
     9481 cgcaggcgca caagcaggat agcatcggcc tgttccgcct gcgaccgggg gttttccccg
     9541 caaaccggca ctaatcccgg tagcggcgcc cgccttcgcg aaagcttaac cgccgctaaa
     9601 catcgcgcgc ggcacggttg accggtgaag gcctccgagg atacagtgcc aatcaggccg
     9661 tttctgtccg gtctctacgg aaggcaacga tcatgggact tcagctcaca cgcggcgccg
     9721 agtacgccat ccgggcgatg tcgtacctcg cgcggttccc cgaggggcac gtcgcctcgc
     9781 tgcacgagat cggcgaggcg caggacatcc ccgagagctt cctggcgaag atcctccaga
     9841 gcctggtgcg cgcgggcctc acggtgtcgc agcggggcgc ccacggcggg ttcgcgctcg
     9901 cgcacccggc ctcggagatc accatgcagc aggtgatcga ggcggtcgac gggcccatct
     9961 ccgtcaacca gtgcgtcctc agccccgagg actgcgcacg cagcgcgtcc tgcaccgtgc
    10021 acgaggcctg gctgcgcgcc cagggtcagc tcatggacgt gctcggctcc atcacgctcg
    10081 agtcgctggc cccgcggccg gcggccgtcg gctgacggcg cggcgccggc actccgccgg
    10141 agcccgcacg cccgaccctc aacgctccgc gtacccgtcc gggtgctcgc gcatccacgc
    10201 ccacgcgtcg gcgacggcgt ccgtgaggct gcgccgcggc cgccagccca ggtgctccgc
    10261 cgcccgccgg ttgctcgcca cgaggaccgg cgggtcgccg gctcgccgcg gcgccgtctc
    10321 gcgcgcgagc gtgagcccgg tgaccgtctc gaccgcgtcc agcaccgcct gcaccgagtc
    10381 gcccgtcccc gtgccgaggt tgaacgagcc ctggacgccg gggagcgcct cgagcgcggc
    10441 gatgtgggcg tcggcgaggt cggcgacgtg cacgtagtcg cgcacgcagg tgccgtctgg
    10501 ggtcgggtag tcgtcaccga acacgcggat cgccgagccg tcgcgggcgg cgcgcagcgc
    10561 cagcgggatg aggtgcgact ccgggtcgtg gtcctcgccg caggcgccgt cggcgccgca
    10621 ggcgttgaag tagcggaggg cggtggcggc gaactcgccg cctgagcgcg cctgctcgag
    10681 caggcgctcc ccggcgagct tggtctcgcc gtacgggttg gcgggcgcca gcgtgtcgcc
    10741 ctctccgatc ggcgtgcgct cgggcgcccc gtacaccgcc gccgtggagc tgaagaccag
    10801 gcggccggcg cctgcggccg cggccgccgc gatcaccgcc gcggtgccgt cgcggttgac
    10861 gctgaagtag cgctccggct cggcgacgga ctcggcgacc tccgccagcg ccgccatgtg
    10921 cacgacggcc tggcagccgc cttcggccag ggcggcgcgc accgcggccg tgtcgcggat
    10981 gtcgccgcgc acgaggggca cgccctcggg gaccgcggcg gcgtgcccgc gcgagaggtc
    11041 gtcgtagaca accacgtcgt ggccgcgcgc cgcgaggcgc gcgacgatga cgctgccgat
    11101 gtagccggcg ccgccggtga cgaggacgcg catgagcgga tgctatcagt gcgggacgag
    11161 aagaggggcg gggggggcc
//
LOCUS       DASUMX010000017         4455 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_148361, whole genome shotgun sequence.
ACCESSION   DASUMX010000017 DASUMX010000000
VERSION     DASUMX010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4455
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148361"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1407
                     /gene="thrC"
                     /locus_tag="VFZ86_00835"
     CDS             <1..1407
                     /gene="thrC"
                     /locus_tag="VFZ86_00835"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEX5640862.1"
                     /translation="VEQIVALADVPYHARAAAVYEAFELDVVAGRTREIAARAYGPNF
                     DDPEVAPVRDVAPGLHVLELWHGPTLAFKDMALQCMPLYFGEALEQAHVKGATDLDFL
                     ILVATSGDTGVAALNGFADRAHTRICVYYPDAGVSALQELQMVTQPGDNLTVFRLGGD
                     FDDCQSSVKAVFDDGDFATELAERHRLSLSSANSINWGRLLPQIVYYLSGYADLAARG
                     AVASGDPIDVCVPTGNFGNILAAWYARDIGVPIRRLLCASNSNNVLYDFLSSGVYDIA
                     ARSLVKTPSPSMDILISSNLERLLYDLTGDPGRIAGWMAELKASGRFEVDAQTRQKLR
                     TSFVGEWVDNDTCLEVIGRVQRERGYLLDPHTAVAWQAAERLAGEAPVLIASTAHWSK
                     FAADVARGLTGVRAGDPVPGAAGDLELLDRVVDLAPGASVPPQLRAVRARPRRFDARV
                     EAGRLPVEESLRAWLAGA"
     gene            complement(1487..2731)
                     /locus_tag="VFZ86_00840"
     CDS             complement(1487..2731)
                     /locus_tag="VFZ86_00840"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX5640863.1"
                     /translation="MKLFRRGDAHAAAAAPATPATAEPKPSGRGFIPRWFARAGVGSW
                     YLIGIVIVLAGLAYALHLLATLVVPTLFAVLLGATFMPAVDWLERHHLGRTWGALIMT
                     VLIVVGAFALLALMTVGVVNQWSTIENQVQAAADWINESLQKLNIDPALIDSAKETLT
                     GSVGSWAQGIFGGAIQGISTLASFAFGAFIGLNILIYVLVGGRRIARWASAHMGPVPP
                     PVGYSILANSARFLRGYIWGSTIIGVFNGAVVALGALVIGVPLAFTIFVVQWLTNYIP
                     MFGAFIGGAFAVLIALGTGGVRDAIWMLIFVLIANGPLQTVVSQFALGASLKLSGLVV
                     LFATTGGAILAGALGGIFAAPFVKIGVDAYHQLEAAGLFDDGPGAAGDAGATAPGEGG
                     PPGAGEGGPPGAVTEAAPPATD"
     gene            2819..4063
                     /locus_tag="VFZ86_00845"
     CDS             2819..4063
                     /locus_tag="VFZ86_00845"
                     /inference="COORDINATES: protein motif:HMM:NF014803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEX5640864.1"
                     /translation="METTIGRFPWEHEGVRVEGGRLLVAGRDAEALAREHGTPLYVFD
                     VPRVAEKARALRDALARAGLTPRVRLALKAQRAPEILAALRALGAPGSPAAVGIDACS
                     PGEVLHALEHGFTAAEISVTGTNLSERDLDVIVPAGVHVNLDLFSQIRRYGRRAPGRA
                     VGLRVNPRGGVMRGHAESLYSGARPTKFGIYEEDLGAAIDAARRHDLTIDTLHVHLAN
                     GMLDDELAAYDSALEPVTRMAEALLAAGCPLVEVNAGGGLGTPLHAGEQPLDLDAWAA
                     VLARRFGHLGVAVGCEPGEFLTNESGIMLAEVVSVEDRLGTTFVGLDAGWNVMNDPYI
                     YDRRPHALVAARALAPRDRETTLAGHINEGDDVFATGVPFPEVSEGEVVAFVSTGGYS
                     PGMWTDHCLRPRAGSVYFADRA"
     gene            complement(4103..>4455)
                     /locus_tag="VFZ86_00850"
     CDS             complement(4103..>4455)
                     /locus_tag="VFZ86_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5640865.1"
                     /translation="TLVQSGSVVIVLLGPAVGPECAALPGAPQPRTLVAAARRLQTGA
                     GRLYGAASREEARMRYGGDLVVLFMLGGGVMFTLLSVAAWVTGRRAGAVFLAVVAGAN
                     LAFALGLLDRASGG"
BASE COUNT          636 a         1661 c         1595 g          563 t
ORIGIN      
        1 gtggagcaga tcgtggccct ggcggatgtg ccctatcacg cgcgtgcggc ggcggtgtac
       61 gaggccttcg aactcgacgt cgtcgccggc aggacgcgcg agatcgcggc gcgcgcctac
      121 ggccccaact tcgacgatcc cgaggtcgcc ccggtccgcg acgtggcgcc ggggctgcac
      181 gtgctcgagc tctggcacgg ccccacgctc gcgttcaagg acatggcgct gcagtgcatg
      241 ccgctctact tcggcgaggc gctggagcag gcgcacgtca agggcgcgac cgacctcgac
      301 ttcctcatcc tggtcgcgac cagcggcgac accggcgttg cggcgctcaa cggcttcgcc
      361 gatcgcgccc acacgcggat ctgcgtctac tatccggacg ccggcgtctc ggccctgcag
      421 gagttgcaga tggtcacgca gccgggcgac aacctgaccg tgttccgcct cggcggcgac
      481 ttcgacgact gccagagctc ggtcaaggcc gtgttcgacg acggcgactt cgccacggag
      541 ctggccgagc ggcaccggct ctccctgagc tcggccaact cgatcaactg gggccgcctg
      601 ctcccgcaga tcgtctacta cctcagcggg tacgccgacc tcgccgcgcg cggcgccgtc
      661 gcgagcggcg accccatcga cgtctgcgtc cccaccggca acttcggcaa catcctggcc
      721 gcctggtacg cgcgcgacat cggcgtgccc atccggcgtc tcctctgcgc gagcaacagc
      781 aacaacgtcc tctacgactt cctctcgagc ggggtctacg acatcgccgc ccgctcgctg
      841 gtcaagacgc cgtcgccgtc catggacatc ctcatctcgt ccaacctcga gcggctgctg
      901 tacgacctca ccggcgaccc cgggcgcatc gccgggtgga tggcggagct caaggcgagc
      961 ggccggttcg aggtggacgc gcagacccgc cagaagctgc gcacgtcctt cgtgggcgag
     1021 tgggtggaca acgacacctg cctcgaggtg atcggccgcg tgcagcgcga gcggggctac
     1081 ctgctcgacc cccacacggc cgtcgcgtgg caggccgcgg agcggctcgc cggcgaggcg
     1141 ccggtgctca tcgcgagcac ggcgcactgg agcaagttcg cggccgacgt ggcgcgcggc
     1201 ctcaccggcg tgcgcgccgg cgatccggtg ccgggagcag ccggcgacct cgagctcctc
     1261 gaccgcgtcg tcgacctcgc gcccggagcg agcgtgccgc cgcagctgcg cgccgtccgc
     1321 gcccggccgc ggcgcttcga cgcccgcgtc gaggccggcc gtctgccggt cgaggagagc
     1381 ctgcgggcgt ggctcgccgg cgcctgaacg ccgccggacg ccccgcacct cagtccggac
     1441 gccttggccc ttcgggccgg gggccgctca ccagcgcttc cgtgcatcag tccgtcgcgg
     1501 gcggcgccgc ctccgtcacg gcccccggcg gcccaccttc accggccccc ggcggccctc
     1561 cttcgcccgg cgccgtcgcg cccgcgtcac cggcggcccc cggtccgtcg tcgaagagac
     1621 cggccgcctc gagctggtgg tacgcgtcca cgccgatctt gacgaacggc gcggcgaaga
     1681 tgccgccgag cgcgccggcc aggatcgcgc cgccggtggt cgcgaagagc accaccaggc
     1741 cgctcagctt gagcgacgct cccagggcga actgcgagac cacggtctgc agcggtccgt
     1801 tggcgatcag cacgaagatc agcatccaga tcgcgtcccg gaccccaccc gtacccagcg
     1861 cgatgagcac cgcgaacgcg cccccgatga aggcgccgaa catcgggatg tagttggtga
     1921 gccactggac cacgaagatc gtgaacgcca gcggcacgcc gatgacgagc gcgccgaggg
     1981 cgaccacggc cccgttgaag acgccgatga tcgtgctgcc ccagatgtag ccgcgcagga
     2041 accgcgcgct gttggcgagg atcgagtagc ccaccggcgg cggcaccggg cccatgtgcg
     2101 cggaggccca gcgagcgata cgccgccctc cgacgagcac gtagatgagg atgttgaggc
     2161 cgatgaaggc gccgaaggcg aagctcgcca gcgtgctgat gccctggatc gcgccgccga
     2221 agatgccctg tgcccagctg ccgacgctgc cggtgagcgt ctccttggcg gagtcgatga
     2281 gggcgggatc gatgttgagc ttctgcagcg actcgttgat ccagtccgcg gccgcctgca
     2341 cctggttctc gatcgtcgac cactggttga cgacgccgac ggtcatgagc gcgagcaggg
     2401 cgaacgcccc gaccacgatg agcaccgtca tgatgagcgc gccccaggtg cgccccaggt
     2461 ggtgccgctc gagccagtcg accgcgggca tgaacgtcgc acccagcaga acggcgaaca
     2521 gggtaggcac gaccagggtc gccagcaggt gcagcgcgta ggcgagcccg gcgaggacga
     2581 tgacgatgcc gatgaggtac cagctgccga cgccggcgcg cgcgaaccac cgggggatga
     2641 agccgcgacc ggatggcttc ggctcagccg tcgcgggcgt cgcgggcgcg gcggcggcag
     2701 cgtgggcgtc gcccctcctg aacagcttca cggcacctcc cgtcggcggc cggccctccg
     2761 gcgtggcgtc atcttacacg ctcgccgctg ggccggtgtc tgcgataggc tcgtttccat
     2821 ggagaccacg atcgggcgct tcccctggga gcacgaaggc gtgcgcgtgg agggcgggcg
     2881 cctgctcgtc gcgggccgcg atgcggaggc gctcgcgcgc gagcacggga cgccgctgta
     2941 cgtcttcgac gtgccgcggg tcgccgagaa ggcgcgcgcg ctgcgggatg cgctcgcgcg
     3001 cgccggcctc acgccccggg tgcgcctggc gctcaaggcg cagcgcgcgc ccgagatcct
     3061 ggccgcgctg cgtgcgctcg gggcaccggg ctcgcccgct gccgtcggca tcgacgcctg
     3121 ctcgcccggt gaggtcctgc acgcgctcga gcacggcttc acggccgcgg agatcagcgt
     3181 caccggcacc aacctgtcgg agcgcgacct ggacgtgatc gtcccggccg gcgtgcacgt
     3241 gaacctcgac ctgttcagcc agatccggcg ctacggccgc cgcgcccccg gccgcgcggt
     3301 cgggctgcgc gtcaacccgc ggggcggcgt catgcgcggg cacgccgaga gcctgtacag
     3361 cggcgcgcgg ccgaccaagt tcggcatcta cgaggaggac ctgggcgccg ccatcgacgc
     3421 cgcccggcgc cacgacctga cgatcgacac gctgcacgtg cacctcgcca acggcatgct
     3481 ggacgacgag ctcgcggcgt acgactccgc cctggagccg gtgacccgga tggcggaagc
     3541 cctgctggcc gcggggtgcc cgctggtgga ggtcaacgcc gggggcggcc tcggcacgcc
     3601 gctgcacgca ggcgagcagc cgctcgacct ggacgcctgg gccgcggtcc tggcgcgccg
     3661 cttcggccac ctcggcgtgg ccgtggggtg cgaacccggc gagttcctga ccaacgagag
     3721 cgggatcatg ctcgccgagg tggtgagcgt cgaggaccgc ctggggacca ccttcgtcgg
     3781 cctcgacgcc ggctggaacg tgatgaacga cccttacatc tacgaccgcc gcccgcacgc
     3841 ccttgtcgcc gcccgcgccc tcgcgccacg cgaccgcgag acgacgctgg ccggccacat
     3901 caacgagggc gacgacgtct tcgccaccgg ggtccccttc ccggaggtga gtgagggcga
     3961 ggtggtcgcc ttcgtctcga ccgggggcta cagcccgggc atgtggacgg accactgcct
     4021 gcggccccgc gccgggagcg tgtacttcgc ggacagggcc tgacgcggtc gccgcggagc
     4081 ggcggcggcc ggaagcgccg gatcagccgc ccgaggcccg gtcgagcagc ccgagggcga
     4141 aggcgaggtt ggcgccggcg acgaccgcca gaaagacggc gccggcgcgg cggcccgtga
     4201 cccaggcggc gacggagagc agcgtgaaca tcacgccgcc gccgagcatg aacagcacga
     4261 ccagatcgcc gccgtaccgc atgcgcgcct cctctctcga tgcggccccg tacagccgcc
     4321 cggccccagt ctgcagccgc cgcgcggccg cgacaagcgt gcgcggttgc ggcgcgcccg
     4381 gcagggccgc acactcgggg ccgaccgccg gcccgaggag gacgatgacg acgctgcccg
     4441 actggacgag cgtgc
//
LOCUS       DASUMX010000018        14227 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1011366, whole genome shotgun sequence.
ACCESSION   DASUMX010000018 DASUMX010000000
VERSION     DASUMX010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 14227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14227
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1011366"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="VFZ86_00855"
     CDS             <1..410
                     /locus_tag="VFZ86_00855"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX5640866.1"
                     /translation="LPQRLVLDIFEEIKGTGGCTLHPPPRVSRLVADAPGLLDLGGLR
                     EAVVREAQREGLEAVAQLEQYEDRFATAAQVVEARLAARLTQRQLAAAAGVSQYEISL
                     LENGSANPTLTTLARLSRALGIELRIGNGGAGG"
     gene            complement(420..1874)
                     /locus_tag="VFZ86_00860"
     CDS             complement(420..1874)
                     /locus_tag="VFZ86_00860"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5640867.1"
                     /translation="MADGSILIVDDEYGVRSGIRTILEMEGYEVGEAGDGAAALRALD
                     ERDYDVALLDYRLPDTDGLSLLQTMKQRGCDAMVCMITAYANIDTAIAATRQGVDFFL
                     PKPFSPDDLEGVVETLLRHKLARTEAERLRAEHEASLLALAEEKTQTHSLVSSLRDAV
                     LVVNRAGDVVLANPPMLQLLGLGEDEVLMRPAQELLGGGALAPLYEQLAAPVKDRTIR
                     QLELDDQTYMASIVTFRDESGEERGRILTLSDISEVRRVAMEKQKFIRMMVHELKSPL
                     GSIKGLLEVVTDKSLGEDLGPYLPMIARADHRIDNLVELIRDLLTLARSEQAPEGEEP
                     ELVPVAPVLDEVVELARERGAQRGVTVTADLAAGLPPALIRPDDLRLVLSNLVGNAVK
                     YNRDGGAVTVSGAVEEGWLRLDVTDTGIGIKEENLARLWDEFFREKRDETRELEGNGL
                     GLSIVKRLVERAGGRLEATSVVGQGSVFTVLLPA"
     gene            complement(1885..2289)
                     /locus_tag="VFZ86_00865"
     CDS             complement(1885..2289)
                     /locus_tag="VFZ86_00865"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5640868.1"
                     /translation="MTTQATVLFVDDDRDFLESKRVYLEARDYRVLTAESPEAALALL
                     ETETPAIIFLDLMMEHVDDGFRLGYRIGKDERLGHIPLVMLSGVAAETGTRFDGEAGG
                     LREWSRLARFLDKPVTGAQVLAVIEELAEQAA"
     gene            complement(2286..4331)
                     /locus_tag="VFZ86_00870"
     CDS             complement(2286..4331)
                     /locus_tag="VFZ86_00870"
                     /inference="COORDINATES: protein motif:HMM:NF014904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HEX5640869.1"
                     /translation="MSGTIITTIKQNCRRCYTCVRDCPAEAIRIEDGQAFVVDERCIA
                     CGNCTLVCSQNAKAYLSGLERASWLLEEDAPVAALLAPSFPAGFSAPAGQVVGAFKAA
                     GFDYVVEVAQGADLVSRAYADYLEANPTGVHIATACPAVAEYVRKYHPEMVDRLVPIV
                     SPMVATALAIKELYGDDVRCVFIGPCVAKKAEARDPQLEPVIDEVLTLQEAYRLLAAR
                     GVDPAKAAEATWDEPAAGRARVFPLPGGLLESAGMDRGMLDPDVLVVSGHDETIEVLD
                     SLSEADTGQTLLVEALMCRGCYCGPGISSSEPGLLRKRRVGEYVAECRRREAPAERAG
                     DDRLAAAFPGLKLSRSYTPDDQRLPEPTEEDIREILARTNKFFPEDELNCGACGYPTC
                     RAKAVAVHCGMAEDAMCLPFMIDQAERVCAELHVPWSEMRDVHRHIINTEKLASMGQM
                     AAGVAHELNNPLSTILLYTHILGKKLQDRDDLDHDLKLVAEESERCKKIVGNLLDFAR
                     QSRVHVERVDVREVVQQAVDSAAATVQPTGHTVELVIDVAPGLRADLDRDQMTQVLVN
                     LVKNGVEAMEGRDGTVCVSANEVEGGGRIHIAVSDEGAGIPGSARDKVFQPFFTTKSI
                     GKGTGLGLPISYGIVKMHNGTIWFDTETDRGTTFHVELPSTHAGGQAALIDDKGATA"
     gene            complement(4342..6060)
                     /locus_tag="VFZ86_00875"
     CDS             complement(4342..6060)
                     /locus_tag="VFZ86_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016695 - oxidoreductase activity, acting
                     on hydrogen as donor [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase, group A"
                     /protein_id="HEX5640870.1"
                     /translation="MKLTINGKEIEAARGETVLKAARRAGVDIPHLCSLDWAPSPAAS
                     CRLCVVEVEGSPRLLTSCTLEVTDGMAVRTHTPRVLKARRAIMELLIASHPQDCLSCV
                     RGGDCELAALAADVGARRRRYVGAVKQHPVDISSPALWRDPDKCVLCGRCVTVCHDVQ
                     GVGAIDFTGRGFTTKVAPGFAVGLNVSECVYCGQCARVCPTGAIVERDQVDDVVAALA
                     DPDAVVVAQVAPAVPATLLEGHSKSEGVRVMLERLAAALKRVGFDAVFDTGFAADLTI
                     MEEAAELLDRVQNGGVLPMFTSCSPAWVNYVETHRPDLIPHLSTCKSPQQMAGALIRE
                     VYPRHADLAGKRLVVVSVMPCTAKKSEAQELSDVDHVLTTRELERLWGRFGIVFDRFT
                     EQAPLDAPFAEATGAGRLFAGSGGVMEAAVRTAAVMAAGQELEGGPKVAEARGAEGVR
                     RFTVTAGDARLNLAVVNGLGRVNESVDGLLAPEAAVHFVEVMSCPGGCVGGGGQPYDT
                     DAQAVRERLERLYEVDRRSRTRRSHENADVQALYEDLLGRPLGEVSHRLLHRTYTDRS
                     PHRAAV"
     gene            complement(6072..8066)
                     /locus_tag="VFZ86_00880"
     CDS             complement(6072..8066)
                     /locus_tag="VFZ86_00880"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEX5640871.1"
                     /translation="MNHSELLTPCCPRCRHTTATPCVDAIACLDHGPICHDEEACRRE
                     RATRLERARRGGDGMTLYVGAGTCGRANGALTVIERMNAYFDRQGLRVPIVETGCVGY
                     CQREVFVDLVTADGVRLSYCDLGPETVDEFLQAVFGDGELHNRFLLGRYDDPDDLYRG
                     VKPIDETPFFARQTKVVLANCGVVDPASLDAALAAGGFRAAARALVTMTPAEVCAEVA
                     ASGLRGRGGAGFPTGQKWKVALDQPRSQKYLICNADEGDPGAFMDRAVLESDPFRVVE
                     GMLIAAYAIGATRGYVYCRAEYPLAIERLGEAIRQCRAAGLLGKDILGSLVDFDITIK
                     MGAGAFVCGEETAILASIEGGRGMPRPRPPYPAVSGLHGCPTVLNNVETLANVPSVVD
                     NGAAWFRDLGYGDAAGTKVFALSGRVRNTGLVEVPVGIPLREVVYDIGGGAPAGHHIK
                     AVQIGGPSGGCIPDEYLDVATDYGTLQELGAMMGSGGMVVMDERSCMVDVAKFFMEFI
                     RNESCGKCTPCREGTTRMHEILSELTERPVGDDLRRLERFRGILHLEELAETVKETSL
                     CGLGQSAANPVLSTLRFFRDEYEAHVLESACPAGACTGLRVFDIDPERCTGCALCAKN
                     CPSGAILGKRKFAHSIIVDRCTGCGACADSCPKQAVLAVA"
     gene            complement(8063..8548)
                     /locus_tag="VFZ86_00885"
     CDS             complement(8063..8548)
                     /locus_tag="VFZ86_00885"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEX5640872.1"
                     /translation="MIVVAATAEAIIAAREGSLTRDQMIPLLQEIQAAEGFISAAAMV
                     QVAEALETPVSRVYGVATFYNQFRFAPLGEHVIEVCRGTACHVKQSLALAQHLKRRLK
                     LRDDGNSADGLYTVVSVACLGACSIAPVIRLDGEFYGHLTTEKLDALLDGVEAGAEEA
                     A"
     gene            complement(8545..8967)
                     /locus_tag="VFZ86_00890"
     CDS             complement(8545..8967)
                     /locus_tag="VFZ86_00890"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5640873.1"
                     /translation="MKAKRILMVDDDRDLVAGVKAFLEARGYDVDTAHSGIEARERLA
                     ARRPDLVVLDIMMDYDTDGFNVAYKLRDEPETESIPVIIMSGFTKELETKTHIFEPMM
                     YREWPAAKFFEKPVKLADLAAAIAELLAGDDAAVAKGA"
     gene            complement(9093..9827)
                     /locus_tag="VFZ86_00895"
     CDS             complement(9093..9827)
                     /locus_tag="VFZ86_00895"
                     /inference="COORDINATES: protein motif:HMM:NF003995.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEX5640874.1"
                     /translation="MSASEQKGFDATTPPQRAAKRVSLQTVERLSVYRKVLEELDRDG
                     VENIHSHQLAELVGVTPAQLRRDLASFGTFGNIARGYNVYQMGRTISRILGTDESQSV
                     ALFGVGDLGRALLSYRGFEERGFHIAVAFDVDSEKVGRVFAGRRCYHVDHLEEVLREF
                     DVHMAILASRPEGLQALVDRVCGAGVHAILNFVPKRVSAPGGCFIEETDIASKLEMLS
                     FLARSEAGSHVASDAPGEPAAEGMTA"
     gene            complement(9934..10818)
                     /locus_tag="VFZ86_00900"
     CDS             complement(9934..10818)
                     /locus_tag="VFZ86_00900"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEX5640875.1"
                     /translation="MDLFALIDARPLLLDGGLGRALMERGFAAGECPEEWNVSHPAEV
                     EDIHRSFYAAGADVVNTNTFGGTAMRLEAHGLSARAVELNEAGARLAVGVRDREFPGR
                     WVAGDVGPCGQMVKPMGNAEPDALRATFAEQARALIAGGVDLLNIETMFDLTEATVAV
                     EAAVAAAAGRPVLASMAFKPAAKGFRTMMGVSPEAAVRALKDAGADLVGCNCEITAEP
                     MAELVRIMADLGGGVTYAQPNAGQPRLEAGETIYGETPEHFAAVVATYPALGAGIVGG
                     CCGTPPASIAALAAALGR"
     gene            complement(10846..11703)
                     /gene="rsmI"
                     /locus_tag="VFZ86_00905"
     CDS             complement(10846..11703)
                     /gene="rsmI"
                     /locus_tag="VFZ86_00905"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEX5640876.1"
                     /translation="MLYVCATPIGNLGDVSPRVLEKLNAAQLVAAEDTRRTRKLLSHF
                     DIHTPLTSFFQHNELQKTDEILRRLRAGETVALVTDAGMPGVQDPGMLLVQKVVKEGL
                     EMTVLPGPSAVLTALVAAGLGGDGFRFLGYLPRRARELEAALKAWQRTGGLVVAFDTG
                     QRLARSLACLAAHLPDARAAVCRELTKVHEEVVRGTLLELSERYPVPGPDVGPAAAVR
                     GEITLVIDLGRPIDEGAARRPGAESAAAALLARGLSRRDTAAALHVCLGMARREADRV
                     AREVAARGG"
     gene            complement(11706..12224)
                     /locus_tag="VFZ86_00910"
     CDS             complement(11706..12224)
                     /locus_tag="VFZ86_00910"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5640877.1"
                     /translation="MSAAPVRIRKAGPDDAAGVAAVLNGVIRGGSPTLLDTTFSVEEE
                     RAYIESLPARSFVHVAETPAGDIVGFQTVIPWNTFVTTAFDHVATMGTYVDAAHRRRG
                     VGAALARRSFAAARALGYDKVFTDLRADNLDSLAYHLALGFAVVGSARGHARVRGRDV
                     DVLFIERSLKEG"
     gene            complement(12221..12964)
                     /locus_tag="VFZ86_00915"
     CDS             complement(12221..12964)
                     /locus_tag="VFZ86_00915"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5640878.1"
                     /translation="MTDDVWREFFDSYAPQYMNEVFTADSLREAAFLVDVLGLEPGAS
                     VLDIGCGTGRHAVELARAGLVVTGLDISSGMLREARRAADAAGVDLELVQGDATTFDL
                     GRAFDAAVCLCEGSFGLLGSDDPFDHDAAVLARAFAALRPGAPLVLTVLNGLRMIRDA
                     TPEQVERGEFDPATLTQAYAMEWQAEDGPRSLPVRERGYVPSELVLLCRSAGFVVEHV
                     WGGTAGDWGRRPVELDEMEVMVVLRKPAT"
     gene            13063..13503
                     /locus_tag="VFZ86_00920"
     CDS             13063..13503
                     /locus_tag="VFZ86_00920"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5640879.1"
                     /translation="MDEPPPRIRPGRAADAAAMAALCTELGYPSSEAQVRARLLRLED
                     VDHGLFVAEGAGGDLAGVIAVHTRLLLEDDPFAELTVLVVTQDARGEGIGSALVAEAA
                     GWARTRGLRRVWVRVNVAREATHRFYESLGFRLYKQQRIYELAL"
     gene            13500..>14227
                     /locus_tag="VFZ86_00925"
     CDS             13500..>14227
                     /locus_tag="VFZ86_00925"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013013777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEX5640880.1"
                     /translation="MKRIVLGDCLDVLAGLDDGCARLVYLDPPFNTGKVQERTRLRTV
                     RDAGGDRTGFKGERYRTEVLGSSAWRDAYEDYLGFLAPRLAEARRILAPDGSLFFHID
                     PREAHYCKVLLDELFGRRSFINEIVWAYDYGARPRSRWPAKHDVIFWYARDPARYVFR
                     YDDIDRVPYLAPGLVGPEKAARGKTPTDVWWQTIVSPTGAEKTGYPTQKPLKILERIV
                     RVHSDPGDLVVDPFAGSGTAGEAAA"
BASE COUNT         1940 a         5110 c         5088 g         2089 t
ORIGIN      
        1 tcctgcccca gagactggtc cttgatatct tcgaagagat caaaggcaca ggtggctgca
       61 cgctccaccc tcctccgcgc gtctcacggc tcgtcgcgga cgccccgggg ctgctggacc
      121 tcggcgggct ccgcgaggcg gtcgtccgcg aagcacagag ggagggtctg gaggccgtgg
      181 cacagctcga gcagtacgag gaccggttcg ccacggccgc acaggtcgtg gaggcgcgcc
      241 tcgcggcgcg gctcacgcaa cggcagctgg ccgcggcggc cggcgtgagc cagtacgaga
      301 tcagcctgct ggagaacggg tccgcgaacc cgaccctgac gacgctggcc cgtctgtcac
      361 gagcactggg gatcgagctc aggatcggca acggcggcgc gggcggctga tcggcggcgt
      421 cacgccggca gcagcacggt gaacacggag ccctggccga cgacgctggt cgcctcgaga
      481 cggccaccgg cgcgctcgac cagtcgcttc acgatcgaga gcccgagccc gttgccctcg
      541 agctctcgtg tctcgtcacg cttctcgcgg aagaactcgt cccagagccg cgccaggttc
      601 tcctctttga tgccgatgcc ggtgtcggtc acgtccagcc gcagccagcc ctcctccacc
      661 gcgccgctca ccgtgacggc gccgccgtcg cggttgtact tgacggcgtt gccgacgagg
      721 ttgctgagca cgaggcggag gtcgtcgggc cggatgaggg cgggcggcag gccggcggcg
      781 agatcggcgg tcaccgtgac gccgcgctgc gcgccccgtt cgcgcgcgag ctccacgacc
      841 tcgtcgagga cgggtgcgac gggcacgagc tccggctcct cgccctccgg cgcctgctcg
      901 ctgcgcgcca gggtgagcag gtcgcggatg agctccacga ggttgtcgat gcggtggtcg
      961 gcgcgggcga tcatcggcag gtacggtccc aggtcctcgc cgaggctctt gtcggtgacc
     1021 acctcgagca ggcccttgat ggacccgagc gggctcttga gctcgtgcac catcatgcgg
     1081 atgaacttct gtttctccat ggcgacgcgc cgcacctcgc tgatgtcgct gagggtgagg
     1141 atgcggccgc gctcctcgcc gctctcgtcg cggaaggtga cgatgctcgc catgtacgtc
     1201 tggtcgtcga gctcgagctg gcggatggtg cggtccttga ccggcgcggc cagctgctcg
     1261 tacagcggcg ccagggcgcc gccgccgagc agctcctgcg ccggacgcat gagcacctcg
     1321 tcctcgccca gtccgagcag ctggagcatc ggcgggttgg cgagcacgac gtcgccggcc
     1381 cggttcacca cgagcacggc gtcccgcagg gagctcacca gcgagtgcgt ctgggtcttc
     1441 tcctcggcga gcgccagcag gctggcctcg tgctcggcgc gcaggcgctc cgcctccgtg
     1501 cgcgccagct tgtggcgcag cagcgtctcc accacgccct cgaggtcgtc ggggctgaac
     1561 ggcttgggca ggaagaagtc cacgccctgg cgcgtggcgg cgatggcggt gtcgatgttg
     1621 gcgtaggccg tgatcatgca gaccatggcg tcgcagccgc gctgcttcat ggtctgcagc
     1681 aggctgaggc catcggtgtc gggcagccgg tagtcgagca gagcgacgtc gtagtcgcgc
     1741 tcgtccaggg cccggagcgc ggcggcgccg tcgccggcct cgcccacctc gtagccttcc
     1801 atctcgagga tcgtgcggat gccgctgcgc acgccgtact cgtcgtcgac gatgaggatg
     1861 ctgccgtcgg ccatggtgga ctccttacgc cgcctgttcg gcgagctctt cgatgacggc
     1921 cagcacctgc gcgccggtga cgggcttgtc gaggaaccgg gcgagccggg accactcgcg
     1981 cagcccgccg gcctcgccgt cgaaccgcgt gccggtctcg gcggcgacgc cgctgagcat
     2041 gacgagcggg atgtggccga gccgctcgtc cttgccgatc cggtacccga gccggaagcc
     2101 gtcgtcgacg tgctccatca tcaggtcgag gaagatgatc gccggggtct ccgtctccag
     2161 cagggcgagc gccgcttccg gggactcggc ggtgagcacg cggtagtcgc gcgcctccag
     2221 gtacacccgc ttgctctcga ggaagtccct gtcgtcgtcc acgaacagga ccgtcgcctg
     2281 ggtcgtcatg ctgtcgctcc cttgtcgtcg atcagcgcgg cctgcccgcc ggcgtgggtg
     2341 ctggggagct ccacgtggaa ggtcgtgccg cgatcggtct cggtgtcgaa ccagatggtg
     2401 ccgttgtgca tcttgacgat gccgtagctg atgggcaggc cgaggcccgt gcccttgccg
     2461 atgctcttgg tggtgaagaa gggctggaag accttgtcgc gggcgctgcc cgggatgccg
     2521 gcgccctcgt cgctgacggc gatgtggatg cgccctcccc cctcgacctc gttcgcgctg
     2581 acgcacacgg tgccgtcccg gccctccatg gcctccaccc cgttcttgac cagattgacg
     2641 agcacctgcg tcatctggtc acggtcgagg tcggcgcgca ggccgggcgc cacgtcgatc
     2701 acgagctcga cggtgtgccc ggtaggctga accgtcgccg cggcggagtc caccgcctgc
     2761 tggaccacct cgcgcacgtc cacgcgctcg acgtgcaccc ggctctgccg cgcgaagtcg
     2821 agcaggttgc cgacgatctt cttgcagcgt tcgctctcct ccgcgacgag cttgaggtcg
     2881 tggtcgaggt cgtcgcggtc ctggagcttc ttcccgagga tgtgggtgta gagcaggatc
     2941 gtgctcagcg ggttgttgag ctcgtgcgcc acgcccgcgg ccatctggcc catggaggcc
     3001 agcttctcgg tgttgatgat gtggcgatgg acgtcgcgca tctcgctcca gggcacgtgg
     3061 agctcggcgc agacgcgctc ggcctggtcg atcatgaaag gcaggcacat ggcgtcctcg
     3121 gccatgccgc agtggacggc gacggccttg gcgcggcagg tcgggtagcc gcaggcgccg
     3181 cagttgagct cgtcctcggg gaagaacttg ttggtccgcg cgaggatctc gcggatgtcc
     3241 tcctcggtgg gctcggggag ccgctggtcg tcgggggtgt agctccggct gagcttcagg
     3301 ccggggaagg cggcggcgag gcggtcgtcc ccggcccgct cggccggcgc ctcgcggcgc
     3361 cggcactccg cgacgtactc gcccacccgg cgcttacgca ggaggccggg ctcggaggag
     3421 ctgatgcccg gcccgcagta gcagccgcgg cacatgaggg cctccacgag cagggtctgg
     3481 ccggtgtcgg cctcactcag gctgtccagc acctcgatgg tctcgtcgtg accgctcacg
     3541 accaggacgt ctgggtcgag catcccgcga tccatgccgg cgctctcgag caggccgccg
     3601 ggaagcggga acacccgcgc ccgcccggcg gccggctcgt cccacgtggc ctccgcggcc
     3661 ttcgccgggt ccacgccgcg agccgccagc aggcggtacg cctcctgcag cgtgagcacc
     3721 tcgtcgatca ccggctcgag ctgcgggtcg cgcgcctcgg cctttttggc cacgcacggg
     3781 ccgatgaaga cacagcgcac gtcgtcgccg tacagttcct tgatggcgag cgccgtggcg
     3841 accatcgggg agacgatcgg cacgaggcgg tccaccatct ccgggtggta cttgcgcacg
     3901 tactcggcca cggccgggca cgcggtggcg atgtggaccc ccgtcgggtt cgcctcaaga
     3961 tagtccgcgt aggcgcggct caccaggtcg gccccctgcg ccacctccac cacgtagtcg
     4021 aacccggccg ccttgaacgc gccgacgacc tgcccggcgg gcgcgctgaa gcccgccggg
     4081 aaggacggcg ccagcagcgc ggccaccgga gcgtcctcct cgagcagcca ggacgcccgc
     4141 tccagcccgc tcaggtaggc cttggcgttc tgggagcaga ccagcgtgca gttgccgcag
     4201 gcgatgcagc gctcgtcgac cacgaacgcc tgcccgtcct cgatgcggat ggcctccgcg
     4261 ggacagtcgc gcacgcaggt gtagcagcgg cggcagttct gcttgatcgt cgtgatgatg
     4321 gtgccgctca tgggactccc gtcagaccgc ggccctgtgc gggctgcggt cggtgtaggt
     4381 gcggtgcagc aggcggtggg agacctcgcc cagcggacgg ccgaggaggt cctcgtagag
     4441 cgcctgcacg tccgcgttct cgtgagaacg gcgcgtgcgg gagcgcctgt ccacttcgta
     4501 gaggcgctcg agccgctccc tgaccgcctg cgcgtcggtg tcgtagggct gcccgccgcc
     4561 gccgacgcag ccgccggggc agctcatgac ctcgacgaag tgcacggcgg cctcgggggc
     4621 caggagcccg tccacggact cattgacacg cccgagcccg ttgaccacgg ccaggttgag
     4681 ccgcgcgtcg ccggcggtga cggtgaagcg gcgcacgccc tcggcgccgc gggcctcggc
     4741 gaccttgggg ccgccctcga gctcctggcc ggcggccatc acggccgcgg tcctgaccgc
     4801 ggcctccatc acgccgccgc tgccggcgaa gagccggccg gcgccggtcg cctccgcgaa
     4861 cggcgcgtcc agcggcgcct gttcggtgaa cctgtcgaac acgatgccga accggcccca
     4921 cagacgttcg agctctcgcg tcgtgagcac gtggtcgacg tcgctcagct cctgcgcctc
     4981 ggacttcttg gccgtgcacg gcatgacgga gacgaccacc aggcgcttgc cggcgaggtc
     5041 cgcgtgccgc ggatagacct cgcggatcag cgcgccggcc atctgctgcg ggctcttgca
     5101 ggtggagagg tgcgggatca ggtccgggcg gtgcgtctcg acgtagttca cccaggcggg
     5161 ggagcaactg gtgaacatcg gcagcacgcc gccgttctga acgcggtcga gcagttcggc
     5221 cgcctcctcc atgatcgtca ggtcggcggc gaagcccgtg tcgaacacgg cgtcgaagcc
     5281 tacacgcttg agcgccgccg ccagccgctc gagcatcacg cgcacgccct cgctcttgct
     5341 gtggccctcg agcagggtgg ccggcacggc cggggcgacc tgcgcgacca ccacggcgtc
     5401 ggggtcggcc agcgccgcga ccacgtcgtc cacctggtcg cgctcgacga tcgcgcccgt
     5461 cgggcacacg cgcgcgcact ggccgcagta cacgcactcg ctgacgttga ggccgacggc
     5521 gaagccgggc gccaccttcg tcgtgaaccc gcgcccggtg aagtcgatgg cgcccacgcc
     5581 ctgcacgtcg tggcagacgg tgacgcagcg gccgcagagc acgcacttgt cggggtcgcg
     5641 ccagagcgcc ggggagctga tgtccaccgg gtgctgcttg acggcgccca cgtagcggcg
     5701 ccggcgagcg ccgacgtccg cggccagcgc cgccagctcg cagtcgccgc cgcgcacgca
     5761 ggacaggcag tcctgcgggt ggctcgcgat gagcagctcc atgatcgcgc gccgcgcctt
     5821 gagcacccgc ggcgtgtgcg tgcgcacggc catgccgtcg gtcacctcga gcgtgcagct
     5881 ggtgagcagc cgcgggctgc cctcgacctc gacgacgcac aagcggcagg aggccgcggg
     5941 gctcggagcc cagtccaggg aacacaggtg cgggatgtcg acgccggccc ggcgcgcggc
     6001 cttgagcacg gtctctccgc gcgccgcctc gatctctttg ccgttgatgg tgagcttcat
     6061 ggtcgttcct ctcaggccac ggccagcacg gcctgcttgg ggcaggagtc ggcgcaggcg
     6121 ccgcagccgg tgcagcggtc gacgatgatg gagtgggcga acttgcgctt gccgaggatc
     6181 gcgccgctcg ggcagttctt ggcgcacagg gcgcagccgg tgcagcgctc cgggtcgatg
     6241 tcgaacacgc ggagcccggt gcaggcgccg gccgggcagg cgctctccag gacgtgcgcc
     6301 tcgtactcgt cgcggaagaa ccgcagcgtg ctgagcaccg ggttcgccgc ggactggccg
     6361 agaccgcaca gcgacgtctc cttgacggtc tcggccagct cctccaggtg caggatgccg
     6421 cggaaccgct cgagccgccg caggtcgtcg ccgaccggcc gctcggtgag ctcgctcagg
     6481 atctcgtgca tgcgcgtcgt gccctcccgg catggcgtgc acttgccgca gctctcgttg
     6541 cggatgaact ccatgaagaa cttggcgacg tcgaccatgc aggagcgctc gtccatgacg
     6601 accatgccgc cgctgcccat catcgcgccc agctcctgga gcgtgccgta gtcggtggcc
     6661 acgtcgaggt actcgtccgg gatgcagccg ccgctggggc cgccgatctg caccgccttg
     6721 atgtggtggc cggccggggc gccgccgccg atgtcgtaga cgacctcgcg cagcgggatg
     6781 ccgaccggga cctcgacgag gccggtgttg cgcacgcggc cggagagggc gaagaccttg
     6841 gtgccggccg cgtctccgta tccgaggtcc cggaaccagg cggcgccgtt gtcgacgacc
     6901 gagggcacgt tggccagggt ctcgacgttg ttgagcaccg tggggcagcc gtgaagaccg
     6961 gagaccgcgg gatagggcgg gcggggacga ggcatgccgc gcccgccctc gatgctcgcc
     7021 agtatcgccg tctcctcgcc gcacacgaag gcgccggcgc ccatcttgat ggtgatgtcg
     7081 aagtcgacga ggctgccgag gatgtctttg ccgagcaacc ccgccgcgcg acactggcgg
     7141 atcgcctcgc ccagccgctc gatcgccagc gggtactcgg cgcggcagta cacgtagccc
     7201 ctggtcgcgc cgatcgcgta ggcggcgatc agcatgccct cgacgacacg gaaggggtcg
     7261 ctctcgagca cggcgcggtc catgaacgcg cccgggtcgc cctcgtcggc attgcagatg
     7321 aggtacttct ggctgcgcgg ctgatcgagg gcgaccttcc acttctgccc cgtcgggaag
     7381 ccggcgccgc cgcgaccgcg caggccggag gcggcgacct cggcgcagac ctcggccggg
     7441 gtcatcgtca ccagcgcgcg ggcggcggcg cggaagccgc cggccgccag cgcggcgtcg
     7501 aggctggcgg ggtcgacgac gccgcagttc gcgagcacga ccttggtctg ccgcgcgaag
     7561 aagggcgtct cgtcgatggg cttgacgccg cggtacaggt cgtcggggtc gtcgtaacgg
     7621 ccgagcagga agcggttgtg caactccccg tcgccgaaga cggcctggag gaactcgtcg
     7681 accgtctccg ggccgaggtc gcagtagctg aggcgcacgc cgtccgccgt caccaggtcg
     7741 acgaacacct cgcgctggca gtagccgacg cagcccgtct cgacgatcgg cacgcgcagc
     7801 ccttggcggt cgaagtaggc gttcatcctc tcgatcacgg tgagcgcgcc gttcgcgcgc
     7861 ccgcaggtcc cggcgccgac gtacagggtc atcccgtcgc cgccgcggcg ggcgcgctcg
     7921 aggcgcgtcg cacgctcgcg ccggcaggcc tcttcgtcgt ggcagatggg gccgtggtcc
     7981 agacaggcga tcgcgtccac gcagggcgtc gccgtggtgt gccggcagcg cgggcagcac
     8041 ggggtcagga gctcgctgtg gttcatgcgg cctcctcggc gccggcctcg accccgtcca
     8101 ggagggcgtc cagcttctcg gtggtgaggt ggccgtagaa ctcgccgtcc agcctgatca
     8161 ccggggcgat cgagcaggcg ccgaggcagg cgacgctgac gacggtgtag aggccgtctg
     8221 ccgagttgcc gtcgtcccgc agcttcagcc gccgcttgag gtgctgggcg agcgcgaggc
     8281 tctgcttgac gtggcacgcg gtgccccggc agacctcgat cacgtgctcg cccagcggtg
     8341 cgaagcggaa ctggttgtag aacgtggcga cgccgtagac gcggctgacc ggcgtctcca
     8401 gcgcctcggc cacctgcacc atcgccgcgg cggagatgaa tccctcggcc gcctggatct
     8461 cctgcagcag cgggatcatc tggtcgcgcg tgagactgcc ctcgcgcgcg gcgatgatgg
     8521 cctcagcggt cgcggccacc acgatcacgc gcccttcgcg accgcggcgt cgtcgccggc
     8581 gagcagctcg gcgatggcgg cggccaggtc ggccagcttg accggcttct cgaagaactt
     8641 ggcggccggc cactcgcggt acatcatcgg ctcgaagatg tgcgtcttgg tctcgagctc
     8701 cttcgtgaac ccgctcatga tgatgaccgg gatgctctcg gtctcgggct cgtcgcgcag
     8761 cttgtaggcc acgttgaagc cgtcggtgtc gtaatccatc atgatgtcga gcacgacgag
     8821 gtcgggccgc cgcgcggcga ggcgctcgcg cgcctcgatg ccgctgtgcg cggtgtccac
     8881 gtcgtagccg cgggcctcca ggaacgcctt gaccccggcc accaggtcgc ggtcgtcgtc
     8941 caccatcagg atgcgcttgg ccttcatgac tctcctcctc gctgtggtac tcgcctcatc
     9001 cggtacggac cggttagtta ccttcttcac agtgaaatcg ttcacagtag gcttgaaaaa
     9061 aacacactcc ccggtcttgg cggcgctcgt cctcacgccg tcatcccctc cgccgccggc
     9121 tcacccggcg cgtccgacgc cacgtggctc ccggcctcgc tgcgcgccag gaacgagagc
     9181 atctcgagct tggacgcgat atcggtctcc tcgatgaagc agccaccggg ggcgctcacg
     9241 cgcttgggca cgaagttgag gatcgcgtgc acgccggcgc cgcacacccg gtcgaccagc
     9301 gcctgcaggc cttccggccg ggacgcgagg atggccatgt ggacgtcgaa ctcgcgcagt
     9361 acctcctcca ggtgatcgac gtggtagcag cggcggccgg cgaagacgcg gccgaccttc
     9421 tcgctgtcga cgtcgaacgc gaccgcgatg tggaagccgc gctcctcgaa gccccgatag
     9481 gagaggagcg cgcggcccag gtcgccgacg ccgaacaggg cgacgctctg gctctcgtcg
     9541 gtgccgagga tgcggctgat ggtgcggccc atctggtaca cgttgtaacc gcgggcgatg
     9601 ttgccgaagg tgccgaagct ggcgagatcg cggcggagct gggcaggcgt gacgccgacc
     9661 agctcggcga gctggtggga gtggatgttc tcgacgccgt cgcggtcgag ctcttccagc
     9721 accttgcgat agacactcag ccgctccacg gtctgcaggg agacccgctt cgcggcccgc
     9781 tgcggcggcg tcgttgcgtc gaagcccttc tgttcgctgg cgctcaaggt tcctcccacg
     9841 gtccctacgg ctgtgattct attcacagta gatggcggct gtcaaccgga tcgtgagcga
     9901 ggggtgaggg agcggcagcg cggccgcgcc aggctagcgg ccgagggccg ccgcgagcgc
     9961 ggcgatggag gcgggcggcg tgccgcagca cccgccgacg atccccgcgc cgagcgccgg
    10021 atacgttgcg acgacggcgg cgaagtgctc cggcgtctcc ccgtagatgg tctccccggc
    10081 ctcgaggcgc ggctgcccgg cgttgggctg cgcgtacgtc accccgcccc ccaggtccgc
    10141 catgatccgg accagctcgg ccatcggctc ggcggtgatc tcgcagttgc agccgaccag
    10201 gtcggcgccg gcgtccttga gcgcgcgcac ggcggcctcg gggctcacgc ccatcatcgt
    10261 gcggaacccc ttggccgcgg gcttgaaggc catgctggcg agcaccggcc gccccgccgc
    10321 agcggcgacg gccgcctcga ccgcgacggt ggcctcggtg aggtcgaaca tggtctcgat
    10381 gttgaggagg tcgaccccgc cggcgatcag cgcacgcgcc tgctcggcga acgtcgcgcg
    10441 cagcgcgtcc ggctccgcgt tgcccatggg cttgaccatc tggccgcaag gcccgacgtc
    10501 cccggccacc cagcggccgg ggaactcgcg gtcgcggacg cccacggcga ggcgggcgcc
    10561 ggcctcgttg agctcgacgg cgcgcgccga cagcccatgc gcctcgaggc gcatggccgt
    10621 cccgccgaag gtgttggtgt tcacgacgtc cgcgccggcg gcgtagaagc tgcggtggat
    10681 gtcctccacc tcggccgggt gcgagacgtt ccactcctcc gggcactcgc cggcggcgaa
    10741 cccgcgctcc atgagggcgc ggcccagccc gccgtccagc agcaacggtc gcgcgtcgat
    10801 gagggcgaag aggtccattg cttcgagtct accagacgag gctcgtcagc ccccgcgggc
    10861 cgccacctcc cgcgcgaccc ggtccgcctc gcggcgcgcc atgcccaggc acacgtggag
    10921 cgccgcggcc gtgtcgcgcc gcgaaagacc gcgcgcgagc agcgccgcgg cggccgactc
    10981 cgcgccgggc cgccgcgcgg ccccttcgtc gatgggccgt ccgaggtcga tcaccagggt
    11041 gatctccccg cgcacggcgg ccgccggccc gacgtcgggt cccggcacgg gatagcgctc
    11101 gctgagctcg agcagcgtgc cgcgcacgac ctcctcgtgc accttggtga gctcgcggca
    11161 cacggcggcg cgggcgtcgg gcaggtgcgc cgccaggcag gcgaggctgc gcgccagccg
    11221 ctggccggtg tcgaaggcga cgaccaggcc acccgtgcgc tgccacgcct tcagcgccgc
    11281 ctcgagctcc cgcgcgcgcc gcggcaggta gccgaggaag cggaagccgt ccccgcccag
    11341 cccggccgcc acgagcgccg tgaggacggc cgaggggccg ggaaggacgg tcatctcgag
    11401 cccctccttc acgaccttct gcaccagcag catgccggga tcctgcactc cgggcatccc
    11461 ggcgtcggtc accagcgcca ccgtctcccc agcgcgcagg cgtcgcagga tctcgtcggt
    11521 cttctgcagc tcgttgtgct ggaagaagct ggtcagcggc gtgtggatgt cgaagtggct
    11581 gagcagcttg cgcgtgcggc gcgtgtcctc cgccgccacc aactgggccg cgttcagctt
    11641 ctcgaggacc cgaggcgaca cgtccccgag gttgccgatg ggcgttgcgc agacgtacag
    11701 cacggtcacc cctccttcag agaacgctcg atgaacagga cgtcgacatc ccggccgcgg
    11761 acgcgggcgt gcccgcgagc ggagccgacc acggcgaacc cgagggcgag gtggtacgcc
    11821 agcgagtcca gattgtcggc gcgcaggtcg gtgaagacct tgtcgtagcc gagggcgcgc
    11881 gcggcggcga agctcctccg cgcgagcgcc gcccccaccc cccggcgccg gtgcgcggcg
    11941 tccacgtacg tgcccatggt cgccacgtgg tcgaacgcgg tggtgacgaa cgtgttccag
    12001 gggatcaccg tctggaagcc gacgatgtcg ccggccggcg tctcggcgac gtgcacgaag
    12061 gagcgcgccg gcaggctctc gatgtaggcg cgctcctcct cgaccgagaa cgtggtgtcg
    12121 agcagcgtcg ggctgccgcc gcggatcacg ccgttcagca cggcggcgac gccggcggcg
    12181 tcgtccggcc cggccttgcg gatgcggacg ggcgcagcgc tcacgtcgcc ggcttccgca
    12241 gcacgaccat gacctccatc tcgtcgagct ccaccggccg gcggccccag tcgcccgcgg
    12301 tgccgcccca cacgtgctcc acgacgaacc cggccgaccg gcacagcagg acgagctcgc
    12361 tgggcacgta gccgcgctcg cgcaccggca ggctgcgcgg cccgtcctcg gcttgccact
    12421 ccatcgcgta cgcctgcgtg agcgtcgcgg ggtcgaactc gccgcgctcg acctgctcgg
    12481 gcgtcgcgtc gcggatcatg cgcaggccgt tgagcaccgt gagcacgagc ggcgcaccgg
    12541 ggcgcagggc ggcgaaggcg cgcgccagca cggcggcgtc gtggtcgaag gggtcgtcgc
    12601 tgccgagcag accgaacgac ccctcgcaga gacacaccgc cgcgtcgaac gcgcgcccga
    12661 ggtcgaacgt ggtggcgtcg ccctgcacga gctcgagatc gacgcccgca gcgtccgcgg
    12721 ccctgcgcgc ctcgcgcagc atgccggacg agatgtcgag accggtgacg acgagaccgg
    12781 cgcgtgccag ctccaccgcg tgccggcccg tcccgcaccc gatgtcgagc acggatgcgc
    12841 ccggctccag cccgagcacg tccacgagga acgccgcctc gcgaaggctg tcggcggtga
    12901 acacctcgtt catgtactgc ggcgcataac tgtcgaagaa ctcgcgccag acgtcgtcgg
    12961 tcatggcccc ctcctcgttc gaccggcccc attctctcag gagcgggccc aaggagcgag
    13021 ggcacccggc caccggctcc gacaggcggt atgctagccg ccatggacga gccgccgccc
    13081 cgcatccgcc ccggccgcgc cgccgacgcc gcggccatgg cggccctctg cacggagctg
    13141 ggctacccgt cgtccgaggc gcaggtccgc gcgcggctgc tgcgcctcga ggacgtcgac
    13201 cacggtctct tcgtcgccga gggcgcgggc ggcgatctgg ccggcgtcat cgccgtccac
    13261 acccgcctgc tcctcgagga cgatccgttc gccgagctca cggtgctcgt cgtcacccag
    13321 gacgcgcgcg gcgagggcat cgggtcggcg ctcgtcgcgg aggccgcggg ctgggcgcgt
    13381 acccgcggcc tgaggcgcgt ctgggtgcgg gtgaacgtcg cgcgcgaggc cacgcaccgg
    13441 ttctacgaga gcctgggctt ccgcctctac aagcagcagc ggatctacga gctcgcgctg
    13501 tgaagcgcat cgtcctgggc gattgcctcg acgtcctcgc cggcctggac gacgggtgcg
    13561 cccggctcgt ctacctggac ccgcccttca acaccggcaa ggtccaggag cgcacgcgac
    13621 tgcgcacggt gcgggacgcc ggcggcgacc gcaccgggtt caagggcgag cgctaccgca
    13681 cggaggtgct cggcagctcg gcctggcgcg acgcctacga ggactacctc gggttcctcg
    13741 cgccgcggct cgccgaggca cggcgcatcc tcgcccccga cgggtcgctc ttcttccaca
    13801 tcgacccgcg cgaggcgcac tactgcaagg tgctgctcga cgagctgttc ggccggcgtt
    13861 ccttcatcaa cgagatcgtg tgggcctacg actacggcgc ccgcccgcgc agccgctggc
    13921 cggccaagca cgacgtcatc ttctggtacg cccgcgaccc ggcccggtac gtgttccgct
    13981 acgacgacat cgaccgggtg ccgtacctgg cgcccggcct cgtgggtccg gagaaggccg
    14041 ctcgcggcaa gacgccgacg gacgtgtggt ggcagaccat cgtcagcccc acgggagcgg
    14101 agaagaccgg ctacccgacg cagaagccgc tcaagatcct cgagcgcatc gtgcgcgtgc
    14161 actccgaccc gggcgacctg gtcgtggacc cgttcgccgg cagcggcacg gccggagagg
    14221 cggcggc
//
LOCUS       DASUMX010000019        12432 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1442762, whole genome shotgun sequence.
ACCESSION   DASUMX010000019 DASUMX010000000
VERSION     DASUMX010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 12432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12432
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1442762"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1280
                     /locus_tag="VFZ86_00930"
     CDS             <1..1280
                     /locus_tag="VFZ86_00930"
                     /inference="COORDINATES: protein motif:HMM:NF025930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEX5640881.1"
                     /translation="MTHERSMEEMDAIGVDFVEKAMARGATQEVADEVFRQLRGFAAY
                     GFNKAHAACFAIVCNASAWLKAHYPAEFYAGILNNQPMGFYSPRVVLNDARRFGLAVL
                     PLDANLSGEWFEVERDGTALRVGLAYVKEMSAVARRAIVAARRERPYTSLSDFVARTR
                     VSREIAENLARLGAFDALGVRREELVAQVPLLYAGARPAGRAAPGGRTSAPPAADAGL
                     DQPRLALDEQLGPLAFLPDWSARRRVGVELELLGLNVSAHPLRFVRDEVEALRVTPMG
                     RLPEVRHGSRVRVAGVLERAQMPWIRSGHRTMFLTLEDETELGQVVVFNDVYLKYGRV
                     LKDAVYLLVDGELQNDEEHGLAVVAHRITDLLRAIGRDEEGGRRASEAVGSTATAGPG
                     AGGASVPGGLTYQRPDAWELPAVEPRTRPGRTG"
     gene            complement(1288..3138)
                     /locus_tag="VFZ86_00935"
     CDS             complement(1288..3138)
                     /locus_tag="VFZ86_00935"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HEX5640882.1"
                     /translation="MNDHDRDPDPAPLPTLLAGRYRIVSPLGSGGMADVYLAEDERLG
                     RPVAVKVLKARLAADDEFLERFRIEARAAASLSHPGIVAVYDRGSDDGATYIAMEYVC
                     GESLKQRLRRDGALPPDEAVSIALAVLSALAAAHARAIVHRDVTSYNVMLEEGGRVVV
                     TDFGIARMGDSALTRTGAMMGTSSYLSPEQAQGRQADERSDLYSLGVVLYEMLAGRVP
                     FRGETDVAVAMQHVSSAPPNPRTLSPGVSEALAGVVMRALSKDPADRYQSAEEFAAAL
                     RTARRPVRPAGGDGVSAAGALAVPGGAVTAGAAGAGLLATGAGVAAATPAPVSFTPAV
                     PSPQAVAPRPPAPVAPSPAAATPAPVPATVVAAEAPTRYRSGGELVTVRPHRRGRTRR
                     VVLAAVLLAGAAAAAWAAYVYLLAPGATVPSVVGEGRADATSAVRGEGLKPALHFVWS
                     DRYGSGEVARQAPRGGAKVDDGVKVDLWVSRGPLHIPSPDLAGLAVEVARDRLEAQSL
                     NSKSRRAASETAPKGEVFRQEPGAGETVRRGDTVTFWVSSGPPRVYVPDVVGYSQGDA
                     SALLEEAGFVVSTDYVAGWGEIPGTVVGQDPLADVKGRVGDEVVIQVAIF"
     gene            complement(3276..4868)
                     /locus_tag="VFZ86_00940"
     CDS             complement(3276..4868)
                     /locus_tag="VFZ86_00940"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEX5640883.1"
                     /translation="MTGTEPSGVPPDAAARRTLFEVEREKRWRVWLLFALLLALAFVT
                     AWVACLLVLGVAYLVFPPVGPPAWLFSLAGIGVVLAAALAFSLLYWGLVQIGARERLL
                     KAMHCRPLDAGDRYHDRLANIVEEMRIATGGPRIECVTVRTVGFNAFAFSDLHGGGVI
                     GVTEGALARLSRQQLQGVVAHELAHILSGSHVTVTVSCLLFGIYSALVEQLDEAADAG
                     ADTDAASAALAMVPLRGWLWAMQVASSVVSAAISRERERQADLAAVRYTRDPLALAEA
                     LRIIGRHPGGAGYIPEGLAPLCIRATGTLGWPAVSWRDTHPPLEQRIAALLGVAGVSG
                     GDFERQAGRAGEAFARREHWAPVPDASGGGLSGAIAAAVAAPATTAVAAAGPAAGRGT
                     PAPPLAGTTCPACGAALAEAPYEGIAIALCERCGGRLVSTPQIGKLLARREAAFTDEQ
                     RRVADLVFSSGDRLRRAAVLARGRSGTGLIACPRCAAPMLRRHYSYEHAVEVDRCVRC
                     DLTWFEKDELEALQILVERQAG"
     gene            complement(5134..5838)
                     /locus_tag="VFZ86_00945"
     CDS             complement(5134..5838)
                     /locus_tag="VFZ86_00945"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEX5640884.1"
                     /translation="MKPLTIVQHEPDDPPGSIAVALGELGVPYEVCRVDEDEALPEWP
                     GGTAGLISLGGAMHATQTKEYPFLAAEIKLMRRIVHEGGPVWGICLGAQLLALADGGD
                     VYRRKHPEIGWTTIEKVADDPLLHGIFSPFVAFNWHAYSCSLPPTAHLIATCGNGVQV
                     FRTGGRSWGTQFHPEVDADMAPHWVRDAVKEQKHLGEAFAEQLRAETEQRLPAYPAFC
                     RRITENFVVNAGLLPA"
     gene            5925..6794
                     /locus_tag="VFZ86_00950"
     CDS             5925..6794
                     /locus_tag="VFZ86_00950"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HEX5640885.1"
                     /translation="MSGSIPTHGPADPQTGAPIVEILSAGNEVLIGDVLDTNTGWLCT
                     RVTELGGLVRRTVMLRDDVDAIGAELRACLARRPALVFTVGGLGPTSDDRTLEGVALG
                     LGARLQLHARAERMVAAKYEEFYAAGHVPFPELNENRRKMARLPAGAEPLVNPIGGAP
                     GVLCRSGDTRVVSLPGVPGELKAIVGESLGPLVAEVFGEARYEERSLEVELQDESAIA
                     DILRAAEEQHPSVYVKSRAKVLGSTRVLRITLSGRGADAAAVSALLAPAAEQLLAQIA
                     AAGFSVRPEAPRP"
     gene            complement(6845..8185)
                     /gene="accC"
                     /locus_tag="VFZ86_00955"
     CDS             complement(6845..8185)
                     /gene="accC"
                     /locus_tag="VFZ86_00955"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015144054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEX5640886.1"
                     /translation="MFSKVLIANRGEIAIRVIRACRELGVGSVAVYSEADADCLHVRY
                     ADEAVCIGPPPAAQSYLVMPALIQAALQSGAEAIHPGYGFLAERAEFSRLCREQGIKF
                     IGPSPESIELMGDKAAARAQMTAAGVPVTPGSEGIIADALEAEVVANDIGLPVMVKAS
                     AGGGGKGIRIVKEASELAAAVRQAQAEAEAAFGNGAVYVEKYIGAPRHIEIQVLADGK
                     GHGIHLGERDCSIQRRHQKLIEEAPSPAVSPELRRQMGDAAVAAAVAAHYEGAGTVEF
                     LLDSDGSFYFMEMNTRVQVEHCVTEMVSGVDIVKTGIRIAAGEGLPLTQDDIELEGHA
                     IEFRINAEDPAEDFMPTPGIVTQWIPPGGPWVRLDSHVYQGYMVPPFYDSLLGKLIVW
                     GRDREECLARSRWALDQFLVDGVKTVIPFHRLVLDHPLFKAGDVNTHFIEDHLG"
     gene            8663..10705
                     /locus_tag="VFZ86_00960"
     CDS             8663..10705
                     /locus_tag="VFZ86_00960"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX5640887.1"
                     /translation="MPSTPTKTLAIIDDDTELLDEVSRALGSAYRVTTSTGDSGTLRM
                     LRDDPPAAVVLDLDLVASDPFELLRLMAGDSALRGVPVLVLSSASDFATFEHAHRAGA
                     SEYVTKPFSPEDLRPKLDGLVNGQKRRTNGRLKLGDLLVASGLVTREQIKQALARQND
                     HGGRLGEVLVADGLVSEQDIITAVAGQMHIGVVDLTEATPQPAALGLLPRDFIVRRRL
                     MPLSLDENGSLVVAMTNPLDVLSIDEIGMRTKKRVVPAICTEAAFDEAVAIYFSSRGK
                     LKDAREEADEPAAGSGEIDADIIEIVDALLTDAASMDASDIHLEPAEDSLNVRCRIDG
                     VLHEMREFPKELQAGIISRLKIMGNLDIAERRLPQDGRTSFELSSGEAVDLRLASIPS
                     MYGENLTIRILEVSPLPPTLASLGLVGENLSRFEAALKAPEGGIVIVGPTGSGKSTTL
                     YTTLELIKTPARKVYTVEDPIERKIEGIIQTEVKPAIGLTFAQALRSLVRADPDVIMV
                     GEIRDLETAKMAADSAITGHQVFSTLHTNDASSAVNRLVEMGVPRYLVAAAWRCFVAQ
                     RLVRRLCPHCRAETTMTDERWSELGLGPAPRKRFRIWEPVGCSKCFGTGYRGRLGLYE
                     VLVVDDALRDLVATGGSVLEIQRMAREKGVSSLREDGIAKVLEGSTSYLELLRVTV"
     gene            complement(10731..11246)
                     /locus_tag="VFZ86_00965"
     CDS             complement(10731..11246)
                     /locus_tag="VFZ86_00965"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX5640888.1"
                     /translation="MSGRRSYLRDVLLLVVVVAVAVPVIVLGARALTGDGDMKMVASP
                     AGSGSPVAAASPAGEPQSCTSCWVDKGAPSPAFAGETAVQDGVQILNVGLVGGYYRPN
                     RFTVQAGVPVKVVFTGYAQDCLGQPEFPELGLKGDMSSGQAVFPLGELEPGAYTFTCS
                     MGVNEGLITVE"
     gene            complement(11274..11681)
                     /locus_tag="VFZ86_00970"
     CDS             complement(11274..11681)
                     /locus_tag="VFZ86_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5640889.1"
                     /translation="MRTYLRAILMVSLVVALLACTASVASARITYFTSKPVTSNAPKK
                     DRPFVTSGYIKPRSTASNRATVKIILWMKYGTGWGVMDTYWTKLSRRPAGGTRYAVTI
                     TIPMKGDHGVQAVQYRKGKKVSKSATRYFYVQP"
     gene            complement(11692..>12432)
                     /locus_tag="VFZ86_00975"
     CDS             complement(11692..>12432)
                     /locus_tag="VFZ86_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5640890.1"
                     /translation="ADTPLDIVFTRPADWASEDPTKHYGAAHFIWGDFEKVDADGLIW
                     HTDGLQPGRYDWMRDFMPKYGTGSLVVAQRIDVDKISHADTHALPYLPADISFGDVSS
                     AGMGCDRSIAFPNPLHRVFIQTSPDPLVWHTDGLVPGRYDWKCWMDDCHYGTLVADGP
                     AQQVPIDSDPLDSVTAVPADTPVDMVFTGARMMCWRTIFFTTAGDFTKTQGYPEPLTW
                     YSGGLAPGSYEWECWMGPQCHHGTIVVQ"
BASE COUNT         1732 a         4573 c         4419 g         1708 t
ORIGIN      
        1 ccatgacgca cgagcgctcc atggaggaga tggacgccat cggcgtcgac ttcgtcgaga
       61 aggcgatggc gcgcggcgcc acgcaggagg tcgccgacga ggtcttccgc cagctccgcg
      121 ggttcgccgc ctacggcttc aacaaggcgc acgccgcctg cttcgccatc gtctgcaacg
      181 cctcggcgtg gctcaaggcg cactacccgg ccgagttcta cgccggcatc ctcaacaacc
      241 agcccatggg gttctactcg ccgcgtgtgg tgctcaacga cgcgcggcgc ttcgggctgg
      301 cggtgctgcc gctggacgcc aacctgagcg gcgagtggtt cgaggtggag cgggacggca
      361 cggcgctgcg cgtcggcctc gcgtacgtga aggagatgag cgccgtcgcg cggcgcgcga
      421 tcgtcgcggc gcgccgcgag cggccctaca cgtcgctcag cgacttcgtc gcgcgcacgc
      481 gggtcagccg cgagatcgcc gagaacctcg cgcgcctcgg cgcgttcgac gcgctcggcg
      541 tacgccgcga ggagctggtc gcgcaggtgc cgctgctgta cgccggcgcg cggccggcgg
      601 ggcgggcggc gccgggcggg cggacgtcag ctccccccgc cgccgacgcc ggcctcgacc
      661 agccgcgcct cgccctcgac gagcagctgg ggccgctcgc cttcctgccc gactggtcgg
      721 cgcggcggcg cgtcggtgtc gagctcgagc tgctcggcct caacgtcagc gcgcatccgc
      781 tccgcttcgt gcgcgacgaa gtcgaggcgc tccgcgtcac gcccatgggg cggctgccgg
      841 aggtgcgcca cggcagccgc gtgcgcgtcg ccggcgtgct cgagcgcgcc cagatgccgt
      901 ggatccgcag cggccaccgc accatgttcc tcaccctcga ggacgagacc gagctggggc
      961 aggtggtcgt cttcaacgac gtctacctca agtacgggcg cgtgctcaag gacgcggtct
     1021 acctgctggt cgacggcgag ctgcagaacg acgaggagca cggcctcgcg gtggtggcgc
     1081 accgcatcac cgacctgctg cgcgccatcg gccgcgacga ggagggcggg cgccgcgcga
     1141 gcgaagcggt cggcagcacg gccaccgcgg gtccgggcgc cggcggggcc tccgtccccg
     1201 gcggcctcac ctaccagcgg ccggacgcct gggagctccc ggccgtggag ccgcgcacgc
     1261 ggccgggccg cacaggctga ccgacgctca gaagatggcg acctggatca cgacctcgtc
     1321 gccgaccctg cccttcacgt cggcgagggg gtcctgcccg accaccgtgc cggggatctc
     1381 tccccacccg gcgacgtagt cggtgctgac cacgaagccg gcctcctcga gcagcgccga
     1441 ggcgtcgccc tgcgagtagc cgacgacgtc gggtacgtag acccttggcg gcccgctgct
     1501 cacccagaac gtcacggtgt cgccgcgcct caccgtctca ccggcgccgg gctcctgcct
     1561 gaacacctcg cccttgggcg cggtctccga ggcggctctg cgactcttgc tgttcagcga
     1621 ctgcgcctcg agcctgtcgc gtgcgacctc gacggccaaa ccggcgaggt ccggagacgg
     1681 gatgtgcagc ggcccgcggc tcacccacag gtcgaccttg accccgtcgt cgaccttggc
     1741 cccgccgcgg ggcgcctgcc gcgcgacctc tcccgacccg tacctgtccg accagacgaa
     1801 gtgcagcgcg ggcttgaggc cctcgccgcg cacggcgctc gtcgcgtccg cgcgcccttc
     1861 gccgaccacg gacgggacgg tcgcgccggg ggcgagcagg tagacgtagg cggcccacgc
     1921 cgcggcggcc gccccggcga gcaggaccgc ggcgagcacg acgcggcgcg tccgcccgcg
     1981 ccggtgcggg cggacggtca cgagctcccc gcccgagcgg taccgggtgg gcgcctccgc
     2041 ggcgaccacg gtcgccggga ccggcgccgg cgtcgccgcc gccggggacg gcgcgaccgg
     2101 ggcgggcggc cggggcgcga ctgcttgcgg cgaggggacg gccggcgtga agctgacggg
     2161 cgccggagtc gcggcggcga ccccggcgcc ggtcgcgagc aggccggctc cggccgcccc
     2221 ggccgtcacc gcccccccgg ggacggccaa cgcgccggcg gcggacacgc cgtcaccgcc
     2281 ggcgggacgc accgggcgcc gcgccgtgcg cagcgcggcg gcgaactcct cggcgctctg
     2341 gtaccggtcc gccggatcct tgctgagcgc ccgcatgacg acgccggcga gcgcctcgga
     2401 cacgcccggc gagagcgtgc gtgggttggg cggcgccgag gagacgtgct gcatcgccac
     2461 ggccacgtcg gtctcaccgc ggaacggtac gcgaccggcg agcatctcgt agagcaccac
     2521 gccgaggctg tagaggtccg agcgctcgtc cgcctgccgt ccctgcgcct gctccggcga
     2581 caggtacgag gacgtcccca tcatcgcgcc ggtgcgcgtg agcgccgagt cccccatgcg
     2641 ggcgatgccg aagtcggtga ccacgacgcg gccgccctcc tcgagcatga cgttgtacga
     2701 ggtcacgtcg cggtgcacga tcgcgcgcgc gtgcgccgcc gccagcgccg acagcacggc
     2761 gagggcgatg ctcaccgcct cgtccggcgg cagcgcgccg tcgcgccgca ggcgctgctt
     2821 gagcgactca ccgcacacgt actccatggc gatgtaggtg gccccgtcgt cgctgccgcg
     2881 gtcgtagacc gccacgatgc cggggtggct gaggctggcc gccgcccgcg cctcgatgcg
     2941 gaagcgctcg aggaactcgt cgtcggcggc cagacgcgcc ttgagcacct tgacggccac
     3001 gggcctgccg aggcgctcgt cctcggcgag atacacgtcc gccatccctc cgctgccgag
     3061 cggcgagacg atgcggtacc gccccgcgag cagggtcgga agcggggcgg gatctgggtc
     3121 gcggtcgtga tcgttcatca ggtcatggcc gtagcgggca gggtcacgca ggcggcgcgc
     3181 tgccgacgtt ggagacgcac caccccgccg ttcggtgcga tggtccagtg tagaggacgg
     3241 tgcggcggct gtcttccgcg cacgggcccg gctgctcagc cggcctggcg ctccacgagg
     3301 atctgcagcg cctcgagttc gtccttctcg aaccaggtga ggtcgcagcg cacgcagcgg
     3361 tcgacctcga ccgcgtgctc gtagctgtag tgccggcgca gcatgggggc ggcgcaccgc
     3421 gggcaggcga tgagacccgt gccggaacga ccgcgagcca ggacggccgc gcggcgcagg
     3481 cggtcgccgc tgctgaagac caggtcggcg acccgccgct gctcgtccgt gaacgccgcc
     3541 tcgcggcgcg ccagcagctt gccgatctgc ggcgtgctga ccagccgtcc gccgcagcgc
     3601 tcgcagagcg cgatcgcgat cccctcgtag ggagcctccg cgagcgcggc gccgcaggcc
     3661 gggcacgtcg tgccggcgag cggcggcgcc ggggtcccgc gtccggccgc cggccctgct
     3721 gccgcgaccg ccgtggtcgc aggggccgct accgcggcgg cgatcgcccc ggagagcccg
     3781 ccgccggacg cgtccgggac cggcgcccag tgctcccggc gcgcgaaggc ctcgccggcc
     3841 cgcccggcct gccgctcgaa gtcgcccccg gagacgccgg cgacgccgag cagcgcggcg
     3901 atgcgctgct ccaggggcgg gtgcgtgtcg cgccacgaga cggccggcca gcccagcgtc
     3961 cccgtggcgc ggatgcacag gggcgccagc ccctcgggga tgtagccggc gccgccgggg
     4021 tgccggccga tgatgcgcag cgcctcggcg agcgccaggg gatcgcgcgt gtagcgcacg
     4081 gcggcgaggt cggcctgccg ctcgcgctcg cggctgatcg ccgcgctcac cacggacgag
     4141 gccacctgca tggcccagag ccacccacgc aacggcacca tcgcgagcgc cgccgacgcc
     4201 gcgtccgtgt ccgcgccggc gtcggcggcc tcgtccagct gctccacgag cgcggagtag
     4261 ataccgaaga gcaggcagga cacggtcacg gtgacgtggc tcccggagag gatgtgcgcg
     4321 agctcgtggg cgacgacccc ctgcagctgc tggcgggaga gtcgcgcgag cgcgccctcg
     4381 gtgacgccga tgacccctcc cccgtgcagg tcgctgaacg cgaaggcgtt gaacccgacg
     4441 gtgcggacgg tgacgcactc gatgcgcggc ccgccggtgg cgatgcgcat ctcctcgacg
     4501 atgttggcga ggcggtcgtg gtaccggtcc ccggcgtcga gcggccggca gtgcatggcc
     4561 ttgagcagcc gttcgcgggc gcctatctgc acgaggcccc agtacaggag cgagaaggcg
     4621 agcgccgccg cgaggaccac tccgatgccg gcgagggaga acagccacgc cggcggcccc
     4681 accggcggga acacgaggta cgcgacgccg aggacgagga ggcacgcgac ccacgccgtc
     4741 acgaaggcga gggcgagcag cagcgcgaac agcagccaga cgcgccagcg cttctcacgc
     4801 tcgacctcga acagcgtccg ccgcgcggcg gcgtccggcg ggacccccga gggttccgtc
     4861 cctgtcatgc ctgccccttc acattgctta acactacggg ggtggtttca actgcgtggg
     4921 tggtggtaat ataccgtctg cgtggtggag tccccctcca cacacccctc ctccacgcag
     4981 ggtacctcca ggtaccaccc catggctcct ttcacaagcc cctctccccg aggggctgcc
     5041 cggaaacggg tgcacgaggc gacgcgcaag cgtcgccttc gtgcgtacgg ggtcaatacc
     5101 tgcgccgggt ctggcgcctc cgcagagcgg ggctcacgcg ggcagcagcc ccgcgttgac
     5161 cacgaagttc tcggtgatgc gccggcagaa cgcggggtac gccggcaggc gctgttcggt
     5221 ctcggcgcgc agctgctcgg cgaacgcctc gcccaggtgc ttctgctcct tgacggcgtc
     5281 gcgcacccag tgcggcgcca tgtccgcgtc cacctcggga tggaactgcg tcccccacga
     5341 tctgccgccg gtgcggaaca cctggacccc gttgccgcag gtcgcgatga ggtgggcggt
     5401 gggcggcagc gagcacgagt aggcgtgcca gttgaaggcg acgaacggcg agaagatgcc
     5461 gtgcagcagc gggtcgtcgg cgaccttctc gatcgtcgtc cagccgatct ccggatgctt
     5521 gcgccggtac acgtcgccgc cgtcggcgag ggcgagcagc tgggcgccga ggcagatgcc
     5581 ccacaccggg ccgccctcgt gcacgatgcg gcgcatgagc ttgatctcgg cggcgaggaa
     5641 cgggtactcc ttggtctgcg tcgcgtgcat ggcgccgccg aggctgatca ggccggcggt
     5701 tccgcccggc cactccggca gcgcctcgtc ctcgtcgacg cggcacacct catacggcac
     5761 gccgagctcg ccgagggcca cggcgatgct gccgggcggg tcgtcgggct cgtgctggac
     5821 gatggtcagc ggcttcatcg cggctcctct ccgttacgta cgggagtata cccagcgcgc
     5881 cgcccgcctt gcccgccccg gccggggtct gcgagactgg cgacatgagc ggcagcatcc
     5941 ccacgcacgg accggccgac ccccagaccg gcgcgccgat cgtcgagatc ctctcggccg
     6001 gcaacgaggt cctcatcggc gacgtgctcg acaccaacac cggctggctg tgcacgcgcg
     6061 tgacggagct cggcgggctg gtgcgacgga cggtgatgct gcgtgacgac gtggacgcca
     6121 tcggcgccga gctgcgcgcc tgtctggccc ggcgcccggc cctggtgttc acggtcggcg
     6181 ggctcgggcc tacgagcgac gaccgaacgc tcgagggcgt ggcgctcggc ctcggggccc
     6241 ggctgcagct gcacgcccgg gccgagcgga tggtggccgc gaagtacgag gagttctacg
     6301 cggccggcca tgtgccgttc ccggagctca acgagaaccg gcgcaagatg gcgcgcctgc
     6361 cggccggcgc cgagccgctg gtcaatccga tcggcggcgc tcccggcgtc ctgtgccgca
     6421 gcggcgacac gcgcgtggtc tcgctccccg gcgtgcccgg cgagctcaag gcgatcgtcg
     6481 gcgagtcgct ggggccgctg gtcgccgagg tcttcggtga ggcgcgctac gaggagcgct
     6541 cgctggaggt ggagctgcag gacgagtcgg ccatcgccga catcctgcgc gccgccgagg
     6601 agcagcaccc gtcggtgtac gtcaagtcgc gcgccaaggt gctcggcagc acgcgcgtcc
     6661 tcaggatcac gctgagcggg cgcggtgcgg acgccgccgc cgtcagcgcg ctgctggcgc
     6721 cggcggcgga gcagttgctg gcgcagatcg ccgccgccgg cttcagcgtc cgcccggagg
     6781 cgccgcgacc gtgacgcccg gcacagagcg ccgcgatggg tgagggtgac gcggcgccga
     6841 ccggtcagcc caggtggtcc tcgatgaagt gcgtgttcac gtcgcccgcc ttgaagagcg
     6901 ggtggtccag cacgaggcgg tggaacggga tcaccgtctt gacgccgtcc acgaggaact
     6961 ggtcgagcgc ccagcgcgag cgcgcgaggc actcctcgcg gtcgcgcccc cagacgatca
     7021 gcttgccgag caggctgtcg tagaacggcg gcaccatgta gccctgatag acgtggctgt
     7081 cgaggcgcac ccacggcccg cccggcggga tccactgcgt gacgatgccg ggtgtcggca
     7141 tgaagtcctc ggccgggtcc tcggcgttga tgcggaactc gatggcgtgg ccctcgagct
     7201 cgatgtcgtc ctgcgtcagc ggcagtccct cgcccgcggc gatgcggatg ccggtcttga
     7261 cgatgtccac gccgctgacc atctcggtga cgcagtgctc cacctggacg cgcgtgttca
     7321 tctccatgaa gtagaagctg ccgtcgctgt cgaggaggaa ctcgacggtg ccggcgccct
     7381 catagtgcgc cgcgacggcg gcggcgacgg cggcgtcgcc catctgccgc cgcagctcgg
     7441 ggctcacggc cggcgacggc gcctcctcga tgagcttctg gtggcggcgc tggatgctgc
     7501 agtcgcgctc gccgaggtgg atgccgtggc ccttgccgtc ggcgagcacc tggatctcga
     7561 tgtggcgcgg cgcgccgatg tacttctcca cgtagacggc gccgttgccg aaggccgcct
     7621 cggcctcggc ctgagcctga cggaccgcgg cggccagctc ggaggcctcc ttgacgatgc
     7681 ggatgccctt gccgccgccg ccggccgacg ccttgaccat caccggcagg ccgatgtcgt
     7741 tggcgacgac ctcggcctcc agcgcgtcgg cgatgatgcc ctcggagccg ggcgtgacgg
     7801 gcacgccggc ggcggtcatc tgcgcccgcg ccgccgcctt gtcgcccatc agctcgatgc
     7861 tctcgggcga cgggccgatg aacttgatgc cctgctcgcg gcagagacgg ctgaactcgg
     7921 cgcgctcggc gaggaacccg tagcccgggt ggatcgcctc ggccccgctc tgcagcgccg
     7981 cctggatgag cgccggcatg accaggtagc tctgcgcggc aggcggcggc ccgatgcaca
     8041 cggcttcgtc ggcgtacctc acgtgcaggc agtcggcgtc ggcttcgctg tacacggcga
     8101 ccgagcccac gccgagctcc cggcaggcgc ggatgacacg gatggcgatc tcgccgcggt
     8161 tggcgatcag gaccttgctg aacacgagcg ctccttggtg ttttgtcgcc ggagtatagc
     8221 gggtgtgcct gcggtgcacc cgcagttgac acgcctacgt aggtcaatag cgggtgtgcc
     8281 tgcggtgcac ccgcagtcga cacgcctacg gcggtcaata gccgaccgat cgagggccga
     8341 ccgatgccgg acctgacaac gctttgggga cggcgaccct caaggccgct gccggggtgc
     8401 cgataggcag gaacgagacc gggtaaggat tactcaccgg gctacggctc ggcatctcat
     8461 cctctctctc agccgggccg gtctccttcg taagcatacg gacagcccgc atccgtgaag
     8521 gcccccgccg gcccggcact ttttctcctc cactccacga cgtccggcgc cgtgcgcgga
     8581 tgtccacccg ccgacgcctc ttcaagcctc ccctccccgc gccgatacgc tagactgacg
     8641 cacgacccag cgggaggtcc gagtgccaag cacgcccacc aagactctgg cgatcatcga
     8701 cgacgacacc gagctcctcg acgaggtctc ccgggcgctc ggctcggcct atcgggtgac
     8761 gacgagcacg ggcgactccg gcaccctccg catgctgcgg gacgaccccc cggcggcggt
     8821 cgtcctcgac cttgatctcg tcgcgtccga ccccttcgag ctgctgcggc tcatggcggg
     8881 cgactccgcg ctgcgcggcg tccccgtgct ggtgctctcc tcggccagcg acttcgccac
     8941 cttcgagcac gcgcaccgcg ccggcgcctc cgagtacgtc accaagccgt tctctccgga
     9001 ggacctgcgg cccaagctgg acgggctcgt gaacgggcag aagcgccgca ccaacggccg
     9061 gctcaagctg ggcgacctcc tggtcgccag cgggctggtg acgcgggagc agatcaagca
     9121 ggcgctcgcc cggcagaacg accacggcgg gcgcctcggg gaggtcctgg tcgccgacgg
     9181 cctggtgagc gagcaggaca tcatcacggc ggtcgccggc cagatgcaca tcggcgtggt
     9241 cgacctcacg gaggccacgc cccagcccgc cgccctcggg ctgctgccgc gggacttcat
     9301 cgtgcggcgc cggctcatgc ccctcagcct cgacgagaac ggcagcctcg tggtcgccat
     9361 gaccaacccg ctggacgtcc tctccatcga cgagatcggc atgcgcacca agaagcgcgt
     9421 ggtgccggcc atctgcaccg aggccgcctt cgacgaggcc gtcgccatct acttcagctc
     9481 gcgcggcaag ctcaaggacg cgcgcgagga ggcggacgag ccggcggccg gcagcggcga
     9541 gatcgacgcc gacatcatcg agatcgtgga cgcgctgctc accgacgccg ccagcatgga
     9601 cgcgtcggac atccacctgg agccggccga ggactccctc aacgtccgct gccgcatcga
     9661 cggcgtgctc cacgagatgc gggagttccc caaggagctg caggccggca tcatcagccg
     9721 cctcaagatc atgggcaacc tcgacatcgc cgagcggcgg ctgccgcagg acggccgcac
     9781 gagcttcgag ctctccagcg gcgaggccgt cgacctgcgc ctcgcctcca tccccagcat
     9841 gtacggcgag aacctcacca tccgcatcct cgaggtctcg ccgctgccgc ccaccctcgc
     9901 gtcgctcggc ctcgtcggcg agaacctctc ccggttcgag gccgccctca aggcgccgga
     9961 gggcggcatc gtcatcgtcg gccccaccgg ctcgggcaag tccacgacct tgtacaccac
    10021 cctcgagctg atcaagacgc cggcgcgcaa ggtctacacg gtcgaggatc ccatcgagcg
    10081 caagatcgag ggcatcatcc agaccgaggt caagccggcc atcggcctca cgttcgcgca
    10141 ggcgctgcgc tcgctcgtgc gcgccgaccc cgacgtgatc atggtcggcg agatccgcga
    10201 cctcgagacg gccaagatgg ccgccgactc cgccatcacc ggccaccagg tgttcagcac
    10261 gctacacacc aacgatgcct cgtcggcggt caaccgcctc gtcgagatgg gcgtgccccg
    10321 ctacctggtg gcggccgcat ggcgctgctt cgtcgcccag cggctcgtgc gccggctctg
    10381 cccgcactgc cgcgccgaga cgaccatgac cgacgagcgc tggagcgagc tcgggctcgg
    10441 cccggcgccg cgcaaacgct tccgcatctg ggagccggtg ggctgctcca aatgcttcgg
    10501 caccggctac cgcggccgcc tcggcctcta cgaggtgctg gtggtcgacg acgccctgcg
    10561 cgacctcgtg gccaccggcg gcagcgtgct cgagatccag cgcatggcgc gcgagaaggg
    10621 ggtgtcgtcg ctgcgcgagg acggcatcgc caaggtgctc gagggctcca ccagctacct
    10681 cgagctgctg cgcgtcacgg tctgaggcgc cgccgcggcg gcggcgccgg tcactccacc
    10741 gtgatcaggc cctcgttcac gcccatgctg caggtgaacg tgtacgctcc gggctcgagc
    10801 tcgcccaggg ggaacaccgc ctgaccgctg ctcatgtcgc ccttcaggcc gagctccggg
    10861 aactcgggct ggccgaggca gtcctgggcg tagcccgtga acacgacctt gaccgggacg
    10921 ccggcctgca ccgtgaagcg gttcggccgg tagtacccgc cgacgagacc cacgttcagg
    10981 atctggacgc cgtcctgcac ggcggtctcg ccggcgaacg cgggcgaggg cgcgcccttg
    11041 tccacccagc agctcgtgca gctctggggc tcgcccgccg ggctcgcggc ggccaccggg
    11101 ctgccgcttc ccgccgggct cgcgaccatc ttcatgtcgc cgtcgcccgt cagggccctg
    11161 gcgcccagca cgatcaccgg cacggccacc gcgaccacga ccacgagcag gagcacgtcg
    11221 cggaggtagg accgccgccc gctcaccagc cccgcctcac tcccgcagcc ggctcacggc
    11281 tggacgtaga agtacctggt cgccgacttg gacaccttct tgcccttgcg gtactgcacc
    11341 gcctgcaccc cgtggtcgcc cttcatgggg atggtgatgg tgaccgcgta cctggtgccg
    11401 ccggccgggc gccgcgacag cttcgtccag taggtgtcca tgacgcccca gccggtgccg
    11461 tacttcatcc acaggatgat cttgacggtc gcgcggttgg acgccgtcga cctgggcttg
    11521 atgtatccgg aggtgacgaa cggcctgtcc ttcttgggcg cgttggacgt caccggcttg
    11581 ctcgtgaagt aggtgatacg ggcgctggcc accgacgccg tgcacgccag cagcgccacc
    11641 accagactca ccatcagaat ggccctcaga tacgtgcgca tgatgtccct cctactggac
    11701 gacgatggtc ccgtggtggc actgcggccc catccagcac tcccactcgt agctgccggg
    11761 ggcgagcccg ccggagtacc acgtcagcgg ctcagggtaa ccttgggtct tcgtgaagtc
    11821 gccagccgtc gtgaagaaga tcgtccgcca gcacatcatc ctcgcgccgg tgaagaccat
    11881 gtcgaccggc gtgtcggccg gcaccgcggt caccgagtcc aggggatccg agtcgatggg
    11941 cacctgctgg gcaggaccgt cggccaccag cgtcccgtag tggcagtcgt ccatccagca
    12001 cttccagtcg tagcgaccgg ggaccaagcc gtcggtgtgc cacacgagcg ggtcggggct
    12061 cgtctggatg aacacacgat gcagggggtt ggggaacgcg atgctgcggt cgcagcccat
    12121 gcccgcggag ctgacgtcgc cgaaggagat gtccgccggc aggtacggca gcgcgtgcgt
    12181 gtcggcgtgt gagatcttgt cgacgtcgat ccgctgcgcg acgaccaggg agccggtgcc
    12241 gtacttcggc atgaaatcgc gcatccagtc gtagcggccg ggctgcagtc catcggtgtg
    12301 ccagatgagc ccgtcggcgt cgaccttctc gaagtccccc cagatgaagt gcgccgcgcc
    12361 gtagtgcttc gtcgggtcct cggaggccca gtcggcgggc ctggtgaaga cgatgtccag
    12421 cggcgtgtcc gc
//
LOCUS       DASUMX010000020         2590 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1874600, whole genome shotgun sequence.
ACCESSION   DASUMX010000020 DASUMX010000000
VERSION     DASUMX010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1874600"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..695
                     /locus_tag="VFZ86_00980"
     CDS             48..695
                     /locus_tag="VFZ86_00980"
                     /inference="COORDINATES: protein motif:HMM:NF024067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3786 domain-containing protein"
                     /protein_id="HEX5640891.1"
                     /translation="MTDPPERRFADALAKARGEWAAADATACAARAGAEVSASGVLVP
                     LFGRPHLVTHPHGAVTAARAGEAAAGPGEPAHVAVAVLLLHYLLTADGAAPTGEWKAY
                     RELPDGLFYATSFAGRAEKPLAGAFAGSPAGLDVFGSAASSSGGEALTLGDASFRFAA
                     LPRVDVAVLVWAGDDEEPGEARVLFDASVSHYLPAEDLAGLGGQLARRLLAAARG"
     gene            803..1144
                     /locus_tag="VFZ86_00985"
     CDS             803..1144
                     /locus_tag="VFZ86_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5640892.1"
                     /translation="MFVRIARFEGGTTAQIEEEGARIRRDLEAARRGESSPEVPQELA
                     LLATRIEMLVDRKRGTVAMLVYAETEEQIREIDRIMREMSPSSDEWGKRVSADLYEVF
                     LDEAPGLAQAA"
     gene            complement(1190..2092)
                     /locus_tag="VFZ86_00990"
     CDS             complement(1190..2092)
                     /locus_tag="VFZ86_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX5640893.1"
                     /translation="MDRRGRDLGAFALALLALIWGYNWVVMKIGLEYAEPFAFSALRT
                     FLGALSLFALLLVLRRPLRPPPLGWTIAIGLLQTTGFVGLLMWALESGGAGKTSVLTY
                     TMPFWLLLLAWAFLGERLHGVQWLAVALALCGLVLVLEPWELEGTFSSALAVAGGFCW
                     AASAVVVKRLQARHEVDILSLTTWQMLFGSLPLVLIAALTWQGGPEWSTDFVLALAYN
                     VILGNALAWFLWLFGLRRLSAGAAGIATLATPVIGVAAAWVQLGERPSAGEAAGMGLI
                     IAALAVLTARGLAAGRRAAPATPD"
     gene            complement(2094..2357)
                     /locus_tag="VFZ86_00995"
     CDS             complement(2094..2357)
                     /locus_tag="VFZ86_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5640894.1"
                     /translation="MRRLSFIVMVGLWGAFIALAFTSPETLDEIWRWTGDLWWPLQIA
                     VWILFLPWMIGLLVWQADWSFAARMAVIVVLALGWSAASFPRR"
BASE COUNT          390 a          943 c          963 g          294 t
ORIGIN      
        1 ggcgaaggcg ccggggctcg ctccggcccg cgatcaggag ggatgaattg acggatccgc
       61 cggaacgcag gttcgccgac gcgctggcga aggcgcgcgg ggagtgggcc gccgcggatg
      121 cgacggcctg tgccgcgcgc gccggcgccg aggtcagcgc ctccggcgtg ctcgtgccgc
      181 tgttcggccg gccccacctc gtcacgcatc cgcacggcgc cgtcacggcg gcgcgggcgg
      241 gcgaagccgc cgccggtccc ggcgagcccg cgcacgtcgc cgtcgccgtc ctgctgctcc
      301 actacctgct gaccgcggac ggggccgctc ccaccggcga gtggaaggcc taccgcgagc
      361 ttccggacgg cctcttctac gcgacgtcgt tcgccggacg ggcggagaag ccgctggccg
      421 gcgcgttcgc agggtcgccc gccggcctcg acgtcttcgg ctccgctgcg agcagctccg
      481 gcggtgaagc tctcacgctg ggcgacgcgt cgttccgctt cgcggcgctg ccgcgggtgg
      541 acgtcgccgt gctcgtgtgg gccggcgacg acgaggagcc cggagaggcc cgcgtgctct
      601 tcgacgccag cgtgagccac tacctcccgg ccgaggacct cgccggcctc ggcggccagc
      661 tcgcccggcg gctgctcgcc gccgcgcgcg gctgagcgcc acgccggcgg gcgtccccgc
      721 ctcgtggttc atctcctgac gctcccgatc cccgtcggcg ggtaccgttc aagtgttcca
      781 tcacctgacg aggaggcgtt ccatgttcgt tcggatcgct cggttcgagg gcggcacgac
      841 cgcccagatc gaggaggagg gcgcgcgcat ccgccgcgac ctcgaggcgg cgaggcgggg
      901 cgagtccagc cccgaggtgc cgcaggagct cgcattactg gccaccagga tcgagatgct
      961 ggtcgaccgg aagagaggca cggtcgccat gctcgtgtac gcggagacgg aggagcagat
     1021 ccgcgagatc gaccggatca tgcgggagat gtcgccctcc agcgacgagt ggggcaagcg
     1081 cgtctcggcc gacctgtacg aggtcttcct cgacgaggcg cccgggctcg ctcaggcggc
     1141 ctgagtcgcg gacggacggg gcgggcgcgg cgagcgcccg ccccgtccgc tagtccggcg
     1201 tcgccggcgc ggctcgcctg ccggctgcga gtccgcgcgc ggtgagtacc gccagcgcgg
     1261 cgatgatgag gcccatgccg gcggcctcgc ccgcggaggg ccgctccccg agctggaccc
     1321 acgcggccgc gacgccgatc accggcgtcg ccagggtggc gatgccggcg gcgcccgcgg
     1381 agaggcggcg caggccgaac agccacagga accaggccag cgcgttgccg aggatgacgt
     1441 tgtaggcgag cgccagcacg aagtccgtgc tccactccgg gccgccctgc cacgtgagcg
     1501 cggcgatgag gaccagcggc agcgagccga agagcatctg ccacgtggtg agcgagagga
     1561 tgtcgacctc gtggcgcgcc tgcaggcgtt tgacgacgac ggcgctggcg gcccagcaga
     1621 acccgccggc caccgccagc gcactgctga acgtgccctc cagctcccac ggctcgagca
     1681 ccaggacgag gccgcacagg gcgagggcga cggcgagcca ctgcaccccg tgaagccgct
     1741 cgcccaggaa cgcccacgcg agcagcagca gccagaacgg catcgtgtac gtgagcacgg
     1801 acgtcttgcc cgcgccgccg ctctcgagcg cccacatcag cagaccgacg aagccggtgg
     1861 tctgcagcag cccgatagcg atggtccagc cgagcggcgg tggccgcagc ggccgccgca
     1921 gcacgaggag gagggcgaag aggctcagtg cgccgagaaa ggtcctgagg gcggagaagg
     1981 cgaacggctc ggcgtactcc aggccgatct tcatgaccac ccagttgtag ccccagatca
     2041 gggcgagcag cgcgagggcg aacgccccga ggtcgcggcc gcggcggtcc acgctaccgg
     2101 cgcgggaacg acgcggccga ccagcccagg gcgagcacca cgatgaccgc catgcgcgcg
     2161 gcgaacgacc agtccgcctg ccagacgagc aggccgatca tccacgggag gaagaggatc
     2221 cagacggcga tttgcagcgg ccaccagaga tcgcccgtcc agcgccagat ctcgtcgagc
     2281 gtctccggag aggtgaaggc gagcgcgatg aacgcgcccc acaagccgac catgacgatg
     2341 aaggacagcc gccgcatgcg gccagcatag cgcgagcggg gaccccggcg cccggcgtgc
     2401 tggtccggct ctcgcgcagc tcctcggccc cggtcagtcg gtgacgggtc gcacgtcggg
     2461 ctcgccggcc gcggccgtac cgcggcggcc ggcgagcagc ccgtgcgcgg cgagcaccgc
     2521 gagcgcggcg atgatgagca ccatgccgat gccctcgacg agcgtcggca cctctcccag
     2581 ctggatccac
//
LOCUS       DASUMX010000021         8481 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_868086, whole genome shotgun sequence.
ACCESSION   DASUMX010000021 DASUMX010000000
VERSION     DASUMX010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 8481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8481
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_868086"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /locus_tag="VFZ86_01000"
     CDS             <1..653
                     /locus_tag="VFZ86_01000"
                     /inference="COORDINATES: protein motif:HMM:NF012379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HEX5640895.1"
                     /translation="VMTMVEEMTAHAAEAVGSLKVTFREHDIDLTPPWPRLTMRAAIE
                     DETGIDIGAEDARTVADLQARIREKGLEDVVPLAPAWGAQVDELFSECVEPRLIQPVF
                     ITEHPLETSPFAKKTPHDPRFVERFEGFIAGMELSNAFSELNDPDDQRERFMQQMQDL
                     ADGDETAHRLDEDFLEAMEHGMPPTGGLGMGIDRLVMVLTGAPSIRDVILFPALRG"
     gene            complement(671..2824)
                     /locus_tag="VFZ86_01005"
     CDS             complement(671..2824)
                     /locus_tag="VFZ86_01005"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX5640896.1"
                     /translation="MRRIITWSVRGRHPWIVIAAWIVVAGLLSLGPRLSSVTSNDASR
                     SLAATVESKRADALQQASFPDAKGTPVIVVFSSDEPLTAADKAAIGAGEEWLMSGAEP
                     VNSARIEYSPDGKGALVFASLDGNPGDESFRDSVQAIRDHFGETVEGMQVRVTGPGGL
                     ITDVYQIFLNADVKLLVGTVILVLVLLLLIYRSPVLPFVPLVAVGFGYFVAGGVLALA
                     ANALDVTLSGQATSLMVILLFGAGTDYGLLLISRYREDLRRESDARVALTTALGETWE
                     AIAASGLTVTLAVLTLLFAQYGDYRSFAPVLGIGVFVTLIAGLTLMPALLALLGRRAF
                     WPRVPKQGEATTHRTWERVAEWVAAGPRRAASLVVLILVVLALGCLFYSPRFSFTEDF
                     LTSMPSSEGYTLLEKHFPKGALAPTTVLFVVPEEPPEFVTGMITGALSESPGVAAAFP
                     TGTGTHSGSTVLGFQVIFEGDPYSSETLEQVRELRVTAREAAATGGGEALVGGPTATQ
                     ADTWAQSNRDTLVVAAVALVVVGAILVILLRALIAPLYLLATNVLSYLAALGATIVIT
                     EKILGWDRISYRIPLYMFIFLVALGSDYNIFITTRIRGEAMARGLRDGTVKALSATGG
                     VLTSAGIILAGTFLILLSQPVKDLAEISIGVALGVLIDTFLVRTALVPGLTLWVGPKA
                     GWPGPLWTRRQTTEEEPADEPRPFAVSGEPVRDEA"
     gene            3051..5444
                     /locus_tag="VFZ86_01010"
                     /pseudo
     CDS             3051..5444
                     /locus_tag="VFZ86_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565137.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
     gene            5670..7088
                     /gene="radA"
                     /locus_tag="VFZ86_01015"
     CDS             5670..7088
                     /gene="radA"
                     /locus_tag="VFZ86_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006862809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEX5640897.1"
                     /translation="MAKATTVFTCGECGRREAKWLGHCPDCGAWDSFVEEVVAAAPKG
                     RGRGKRTGDGAGGGTAAGPVRLSEVVSSAAERFSSGIDELDRVLGGGIVPGSLVLVGG
                     EPGIGKSTLLLQVLDNVAAGRPALLVCGEESAAQVKMRAERICRGAANIDVLAETELE
                     TVLEAVAAARPALVVVDSVQTLYSDAFTSAPGSVSQVREAAGRFLRLAKDTGAAVFLV
                     GHVTKEGAIAGPRVLEHMVDTVLQFEGDRYRFYRTLRAAKNRFGSTNELGIFEMTGSG
                     LKTVPDPSALFLSEAAPAPGAAVHVAVEGTRCFLVEVQALVNQTELAMPRRVAVGFDR
                     NRLAMLVAVLGRHAGLKLSSSDLFVTIAGGIRIDEPAADLAVALAVASAQRDVPLPTG
                     AAMFGEISLTGGLRSCSQSERRIAEAGRAGFDCIVVPAADARRAGDRVAGAADVRGAF
                     AALLGQSADRPASGATNDEWGV"
     gene            7088..7624
                     /locus_tag="VFZ86_01020"
     CDS             7088..7624
                     /locus_tag="VFZ86_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HEX5640898.1"
                     /translation="MYSVGDKVVYPHHGAGKIMKIEQKEVLGQQRDYLTIQILHNDMT
                     VMVPVENADRAGLRKVIESDVVERVLEVLRGDPTKMPKNWSRRYKHNRDKLKTGDIFE
                     VAEVVRNLAIRHADKGLSTGEKQMFSKAKKILASELMYARDFDEDEANAFLETVLEDI
                     HVDGPALADDSREPAPVV"
     gene            7587..>8481
                     /locus_tag="VFZ86_01025"
     CDS             7587..>8481
                     /locus_tag="VFZ86_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5640899.1"
                     /translation="MPTTPESPLPSSETGAAGVPRRPRGVLQPAIRGVLALLGALAGY
                     QLADRIRASSFAPALDQNGKVIWLVAFTASGLVLGYLLGVVLSRLLRRGLDRVDEVMA
                     RATGAEVVVAVGGAVVGLFIATLISIALVRIPIVGPYLTVPIYLLSGYFTAYVAAKKH
                     IEVLRLLGVRGAFEVIPGLTPAKLLDSSAIIDGRILEIIDTGFVEGELLVPRFVVEEL
                     QRLADSSDAEKRVRGRRGLDFVKRLQNSSGAVRIDGADYPDIEAVDAKLVRLGHDTGA
                     MIVSTDYTLKKVAEIHGVKMLN"
BASE COUNT         1383 a         2959 c         2975 g         1164 t
ORIGIN      
        1 acgtcatgac gatggtcgag gagatgacgg cgcacgcggc cgaggccgtc ggatcgctca
       61 aggtcacgtt ccgcgagcac gacatcgacc tcacgccccc gtggccgcgc ctcacgatgc
      121 gcgcggccat cgaggacgag accggcatcg acatcggcgc ggaagacgcg cgcacggtcg
      181 ccgacctgca ggcgcggatc cgcgagaagg ggctcgagga tgtcgtgccg ctcgcccccg
      241 cctggggcgc ccaggtcgac gagctgttca gcgagtgcgt cgagcccagg ctcatccagc
      301 ccgtgttcat caccgagcac ccgctcgaga ccagcccgtt cgccaagaag acgccgcacg
      361 acccgcgctt cgtcgagcgg ttcgagggct tcatcgccgg catggagctg agcaacgcgt
      421 tcagcgagct caacgacccc gacgaccagc gcgagcgctt catgcagcag atgcaggacc
      481 tcgccgacgg cgacgagacc gcgcaccggc tcgacgagga cttcctcgag gccatggagc
      541 acggcatgcc gcccaccggc ggcctcggca tgggcatcga ccgcctggtg atggtgctca
      601 ccggcgcgcc cagcatccgc gacgtcatcc tcttcccggc gctgcgcggc tgacgccggc
      661 gccgccggcg ctaggcttcg tccctcaccg gctcgccgct caccgcgaac ggtcgtggct
      721 cgtccgccgg ctcttcctcc gtcgtctgcc gcctggtcca cagcggcccc ggccagccgg
      781 ccttgggccc cacccacagc gtgagccccg gcaccagcgc cgtgcgcacc aggaacgtgt
      841 cgatgagcac gccgagcgcc acgccgatgc tgatctcggc gaggtccttg accggctgtg
      901 agagaaggat caggaacgtg ccggcgagga tgatcccggc gctggtgagc acgccgccgg
      961 tggccgagag cgccttcacc gtgccgtcgc gcaggccgcg cgccatcgcc tcgccgcgga
     1021 tgcgcgtcgt gatgaagatg ttgtagtcgc tgccgagcgc gaccaggaag atgaacatgt
     1081 acagcgggat gcggtagctg atgcggtccc agccgaggat cttctcggtg atgacgatgg
     1141 tcgctcccag cgccgcgagg taggagagca cgttcgtcgc caggaggtag agcggcgcga
     1201 tgagggcgcg cagcaggatc acgaggatcg cgcccaccac cacgagggcc accgccgcga
     1261 ccacgagtgt gtcgcggttg ctctgcgccc aggtgtcggc ctgggtggcg gtggggccgc
     1321 cgacgagggc ttcgccgccg ccggtcgccg ccgcctcgcg cgccgtcacg cgcagctcgc
     1381 gcacctgctc gagggtctcg ctggagtacg ggtcgccctc gaagatgacc tggaacccga
     1441 gcaccgtgct gcctgagtgc gtcccggtgc cggtggggaa ggcggcggcc acgcccgggc
     1501 tctcgctgag cgcgccggtg atcatgccgg tgacgaactc cggcggctcc tccggcacca
     1561 cgaagaggac cgtggtcggc gccagcgcac ccttgggaaa gtgcttctcc agcagcgtgt
     1621 agccctcact cgagggcatg ctggtgagga agtcctcggt gaagctgaag cgcgggctgt
     1681 agaagaggca gccgagggcc agcacgacga ggatgagcac gaccaggctc gcggcccgcc
     1741 tcggccccgc ggccacccac tccgcgacgc gctcccaggt gcgatgcgtc gtggcttcgc
     1801 cctgcttggg cacgcgcggc cagaaggcgc ggcggccgag cagggcgagc agcgccggca
     1861 tgagcgtgag cccggcgatg agcgtcacga acacgccgat cccgagcacc ggcgcgaacg
     1921 agcggtagtc gccgtactgg gcgaagagca gggtgaggac cgccagcgtc acggtcaggc
     1981 cgctggcggc gatggcctcc caggtctcgc ccagcgccgt ggtcagggcg acacgcgcgt
     2041 cgctctcgcg gcgcaggtcc tcgcggtagc gcgagatgag cagcaggccg tagtcggtgc
     2101 cggcgccgaa cagcaggatc accatgagcg aggtcgcctg gccggagagg gtgacgtcga
     2161 gcgcgttcgc ggcgagcgcg aggacaccgc cggcgacgaa gtagccgaag ccgacggcga
     2221 cgagcggcac gaagggcagc accggcgagc ggtagatgag gagaagcagc accagcacca
     2281 ggatgaccgt gcccacgagg agtttgacgt ccgcgttcag gaagatctgg tagacgtcgg
     2341 tgatcaggcc gccggggccg gtgacgcgca cctgcatgcc ctccacggtc tcgccgaagt
     2401 ggtcgcggat ggcctgcacg gaatcccgga aggactcgtc gccggggttg ccgtcgaggc
     2461 tcgcgaagac gagcgcgccc ttgccgtccg gcgagtactc gatgcgggcg ctgttgacgg
     2521 gctcggcgcc gctcatgagc cactcctcac cggcgccgat cgccgccttg tcggcggcgg
     2581 tgagcggctc gtccgagctg aagacgacga tcaccggcgt gcccttggcg tcggggaacg
     2641 acgcctgctg cagcgcgtcc gcacgcttgc tctccacggt cgccgcgagg gacctggagg
     2701 cgtcgttgct ggtcacgctg gagagcctgg ggcccaggga gaggaggccg gcgaccacga
     2761 tccaggcggc gatcacgatc caggggtgcc tgccgcgtac cgaccaggtg atgatgcgac
     2821 gcatgtgcag gctcctctcc gcgtgggacg gcctctccgt ggcccacatc atactggccg
     2881 ggcgagcgtc ccgcggggtc gcgtcgcgca gggaatcggg taccatagac ccacgtcgtg
     2941 tctccggtgg ggcgagagcg ggtatcccgg agactgagag caagcaccgg aagccgtccc
     3001 tgtccccccc gacgaccccg acggttcccg tatcgtgaag gagtggccgc atgttcgaac
     3061 gcttcaccga acgcgccaga caggtcgtgg tcctcgccca ggaagaggcc cggtccctca
     3121 agcacaacta catcggcacc gagcacctgc tgctcggcct gctccgcgag gaggagggcg
     3181 tcgccgcgcg cgtgctcgac ggcctcgaag tgtcggtcga ggaggtccgc gcggcggtcg
     3241 tccgcatcgt cggctcgggc gaggagtcgc cccagggcca gatccccttc accccgcgcg
     3301 ccaagaaggt cctcgagctg gcgctgcgtg aggctctgag cctcggccac aactacatcg
     3361 gcaccgagca catcctgctc ggcctcgtgc gtgaggacga gggcgtcgcc gcgcgcatcc
     3421 tgctcgacct ggacgccgag cccgagaaga tccgcggcga ggtcatgcgc gcgctcagcg
     3481 gccccggccg ccgcagcgcg cagggcggcg gcgcgtccga gggcaagcgc gcggccaagg
     3541 tcctcgacca gttcggccgc aacctcacca agctcgccga ggagggcaag ctcgacccgt
     3601 gcatcggccg ccagaccgag atcgagcgcg tcatgcagat cctcagccgg cgcaccaaga
     3661 acaaccccgt gctcatcggc gagccgggcg tcggcaagac ggccatcgtc gagggcttcg
     3721 cccagcgcat cgccaaccac gaggtccccg agctgctcaa gggcaagcag gtgtggacgc
     3781 tcgacctggc agcgctcgtc gccggcagca agtaccgcgg cgagttcgag gagcgtctca
     3841 agaaggtcat gaaggagatc cgcgagacgg gcgacatcat cctgttcatc gacgagctcc
     3901 acaacctcgt cggcgcgggg gccgccgagg gcgccatcga cgccgccagc atcctcaagc
     3961 cggcgctggc gcgcggcgag ctccagaccg tcggcgccac gaccctggac gagtaccgca
     4021 agtacctcga gcgcgacgcc gccctggaac ggcgcttcat gcagatcaag gtggccgagc
     4081 cgtcggtcga ggagaccgtc gagatcctca agggcctccg cgaccgctac gaggctcacc
     4141 accgcatccg catcaccgac gaggccctca cctcggcggc cgagctcgcc gaccgctaca
     4201 tcagcgaccg gttcctgccg gacaaggcga tcgacctcat cgacgaggcc gccagccgca
     4261 tgcgcatccg cacgatgacg gcgccgccga gctatcgcga gctcgaggag gagatctcct
     4321 cggtgcgcaa ggagaaggag gcggcgatcg aggcgcagga gttcgagaag gcggccaacc
     4381 tgcgcgacaa ggagcgcaag ctgagccaca ggaagctcga gctcgaggac gcctggaaga
     4441 cgagcgatga gggcatgcgc gccagcatcg gcgaggacga gatcgccgag atcgtgagca
     4501 tgtggaccgg catcccggtg gtcaagatca ccgaggccga gaccaagaag ctgctgcgga
     4561 tggaggacga gctgcacgcg cggctcatcg gccaggaccc ggcggtgcgc gccgtcagca
     4621 aggcgatccg ccgggcgaga gccgggctca aggaccgtcg ccgtcccacc ggctccttca
     4681 tcttcctcgg cccctcgggc gtggggaaga cggagctggc ccgcacgctg gcgaacttcc
     4741 tcttcggcga cgaggaccac ctcgtgcaga tcgacatgag cgagtacatg gagaagcacg
     4801 ccgtgtcgcg tctggtcggg tcgcctcccg gctacgtcgg gcacgacgag ggcggccagc
     4861 tcaccgagat cgtgcgccgc aagccgtaca gcgtgatcct cttcgacgag atcgagaagg
     4921 cgcacccgga cgtgttcaac atcctcctgc agatcctcga ggacgggcgc ctcaccgacg
     4981 cgcagggtcg caccgtcgac ttccgcaacg cgatcctcat catgaccagc aacatcggcg
     5041 cggagatgat caccaaggac acgccgctgg gcttcacgca gacggccaac aagggcatga
     5101 cctacgacga catgaagggc cgcatcaccg gcgagctcaa gcgcgtcttc aagcccgagt
     5161 tcctcaaccg cgtcgacgag gtcatcgtgt tccacaagct ggagcgcgcg gagatcgcca
     5221 agatcgtcga cctgatgatc tcgcggctgc ggcagcagct cgccgtgagc ggtgtggaca
     5281 tccagctcac cgacgccggc cgcgacgtgc tcgtcgagaa ggggtacgac cccatgctcg
     5341 gcgcgcgccc gctgcgacgc gccatccagc ggtacatcga ggacaagctg agtgacgcga
     5401 tgctcgagcg ggcgttcccc gagggcaccg tggtgatcgt cgacggccag aacgaagaca
     5461 ccgtgctcac cgtgaacggc gaggtcgtcg gcggcagcgt cgagctgccg atggtcggcc
     5521 tcgacgaacc ggtgtcgcag ggcgtcggcc cggtcgggac gccgtccggc ggggagagcg
     5581 acggacccga cgccggcggc gcgtccgcgg gcgcctgaga ccgcggtgac ggcgcccgcg
     5641 cctgcgcacg gcgggagccg ctgagagcga tggcgaaggc gacgaccgtc ttcacctgcg
     5701 gcgagtgcgg tcgccgggag gccaagtggc tgggccactg ccccgactgc ggcgcgtggg
     5761 actcgttcgt cgaagaagtg gtcgcggcgg cgcccaaggg gcgtggtcgc ggcaagagga
     5821 ccggagacgg ggccggcggc ggtaccgccg ccggccccgt ccgtctgtcc gaggtcgtct
     5881 cgtcggccgc cgagcgcttc tcgagcggga tcgacgagct cgaccgcgtg ctcggcggag
     5941 gcatcgtccc gggctctctc gtgctggtcg gcggcgagcc cggcatcggc aagtcgacgc
     6001 tgctgctgca ggtgctcgac aacgtcgccg ccggccgccc ggcgctgctc gtctgcggcg
     6061 aggaatcggc ggcgcaggtc aagatgcgcg ccgagcgcat ctgcaggggc gccgccaaca
     6121 tcgacgtcct cgccgagacc gagctcgaga cggtcctcga ggcggtcgcc gcggcgcggc
     6181 cggcgctggt ggtcgtcgac tccgtgcaga cgctctacag cgacgcgttc acgtcggcgc
     6241 cgggcagcgt gagccaggtg agggaggcgg cgggccgctt cctccgcctg gccaaggaca
     6301 ccggcgccgc cgtcttcctg gtcgggcacg tcaccaagga gggcgcgatc gccggccccc
     6361 gggtgctgga gcacatggtc gacaccgtgc tccagttcga gggcgaccgc taccgcttct
     6421 accgcacgct gcgcgccgcc aagaaccggt tcggctccac caacgagctg gggatcttcg
     6481 agatgaccgg gtcgggcctc aagacggtgc ccgacccctc cgcgctcttc ctcagcgagg
     6541 cggcgccggc acccggcgcc gccgtccacg tcgccgtcga gggcacgcgc tgctttctcg
     6601 tcgaggtcca ggcgctcgtc aaccagaccg aactggccat gccgcgacgg gtcgcggtcg
     6661 gcttcgaccg caaccgtctc gccatgctcg tcgccgtgct cgggaggcat gccgggctga
     6721 agttgtctag ttccgacctt tttgttacaa ttgcaggcgg aattcgtatc gacgagcctg
     6781 ctgcggacct cgcggtcgca ctcgctgtcg cctcagcgca gcgcgatgtt ccgctcccca
     6841 cgggggcggc gatgttcggg gagatcagcc ttacgggcgg acttcgatcc tgcagccaga
     6901 gcgagcgacg tatcgccgaa gccggccgcg ccggcttcga ctgtatcgtc gttccggccg
     6961 ccgatgcacg ccgggccggc gaccgtgtcg ccggcgcggc cgatgtgcgt ggggcgttcg
     7021 ccgctctgct cggccagagc gcggacaggc cggcctccgg ggccacgaac gacgagtggg
     7081 gggtttgatg tattcagtcg gagacaaggt ggtctacccg caccacggcg ctggcaagat
     7141 catgaagatc gagcagaagg aagtgctcgg ccagcagcgc gactacctca ccatccagat
     7201 cctgcacaac gacatgaccg tcatggtccc cgtcgagaac gccgaccgcg ccggcctgcg
     7261 caaggtcatc gagtccgacg ttgtcgaaag ggtcctcgag gtcctgcgcg gcgaccccac
     7321 caagatgccc aagaactgga gccgtcgcta caagcacaac cgcgacaagc tcaagaccgg
     7381 cgacatcttc gaggtcgccg aagtggtccg caacctggcc atccgccacg ccgacaaggg
     7441 gctgtcgacc ggcgagaagc agatgttctc caaagccaag aagatcctcg ccagcgagct
     7501 catgtacgcg cgggacttcg acgaggacga ggccaacgcg ttcctggaga ccgtgctcga
     7561 ggacatccac gtcgacggac ccgcacttgc cgacgactcc cgagagcccg ctcccgtcgt
     7621 ctgagaccgg cgccgcgggc gtcccgcgca ggccgcgcgg cgtcctgcag ccggctatcc
     7681 gcggggtcct cgccctgctc ggcgcgctcg ccggctacca gctcgccgac cgtatccgtg
     7741 cgtcgtcgtt cgcgcccgcg ctcgaccaga acggcaaggt catctggctg gtcgccttca
     7801 cggcctccgg tctcgtgctc gggtaccttc tcggcgtggt cctctcgcgc ctgctgcgtc
     7861 gcggcctcga ccgcgtggac gaggtcatgg cgcgcgccac gggcgccgag gtcgtggtcg
     7921 ccgtcggcgg cgcggtcgtc gggctcttca tcgccacgct catcagtatc gcgctcgtcc
     7981 gcatccccat cgtggggccg tacctcacgg tgcccatcta cctgctgtcg gggtacttca
     8041 ccgcctacgt cgccgccaag aagcacatcg aggtcctgcg gctgctcggc gtgcgcggcg
     8101 cgttcgaggt catcccgggg ctcacgccgg ccaagctgct cgactcgagc gcgatcatcg
     8161 acggccgcat cctcgagatc atcgacaccg ggttcgtgga gggggagctc ctcgtgccgc
     8221 ggttcgtcgt cgaggagctc cagcggctcg ccgattcgtc ggacgcggag aagcgcgtgc
     8281 gtgggcgtcg cggcctcgac ttcgtcaagc gtcttcagaa cagctccggc gcggtgcgca
     8341 tcgacggcgc cgactatccg gacatcgagg cggtggacgc caagctggtc cggctcgggc
     8401 acgacaccgg ggcgatgatc gtcagcaccg actacacgct caagaaggtc gccgagatcc
     8461 acggcgtcaa gatgctcaac g
//
LOCUS       DASUMX010000022         8528 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1731438, whole genome shotgun sequence.
ACCESSION   DASUMX010000022 DASUMX010000000
VERSION     DASUMX010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 8528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8528
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1731438"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2056
                     /locus_tag="VFZ86_01030"
     CDS             <1..2056
                     /locus_tag="VFZ86_01030"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010585735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEX5640900.1"
                     /translation="GGLLMLVELNAEQLAAAQHGDGPLLIVAGAGTGKTAALVHRVAH
                     LIERGADPRRILLLTFTRRAAAEMLRRVEAVLAARGHGSGGASERVWGGTFHAGAARL
                     LRIHARDIGMQPDFTIMDRGDSEDLMHVARTRLGLGRGGSRFPQKSTCLDVYSRCVNA
                     QLPLGEVLHTAFPWCLPAEEGLAQLFAAYTDAKEEQQVLDYDDLLLFWRGLLADPGGG
                     ARVRERFDHVLVDEYQDTNALQADIVSLLRPDGTGVTCVGDDAQAIYGFRAATVRNIL
                     DFEQRFPGAAVVTLTQNYRSTAPILAATNALIAEAAERRDKELRTDRAGGGRPVLATC
                     RDEDEQTQWVCEHILAHREQGTQLKEQAVLYRAQHHSMALEMELSRRNIPFHKYGGLR
                     FVEMAHVKDLVSFLRLAENPKDAMAGLRVLGLVPGIGPKTAAALLEALGAAAGDFAAA
                     WTGARVPEPARAAWPAVVELFATLAAAGPSEVPAQVHAVRSVYGPLLERRYDNVQARL
                     RDLEQIEGLAARSQTRSQFLAELVLDPPAYTQELAGPPLLDEDYLILSTMHSAKGLEF
                     DVVYVIHAADGNIPSDMATGSAAEIEEERRLLYVACTRAREQLYVSHPLRYYTQPWAK
                     ADTHGYAQRSRFLTHAVTAHFDEVQTGPEHAPSEEGPLGRVTTASIRAGVRSLWEAPA
                     AG"
     gene            2139..3758
                     /locus_tag="VFZ86_01035"
     CDS             2139..3758
                     /locus_tag="VFZ86_01035"
                     /inference="COORDINATES: protein motif:HMM:TIGR04275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5640901.1"
                     /translation="MPRLFATFVCSSLLIIVMLASPAGASPPFAQETPIAAGPGAQQS
                     PDLGPSLWNWPLTWETDASGVWHAGWRDSTQTETVSTAGGAQRHPAVFQDRLVYEDDR
                     NGTVDLYRTWLALAGYPTPTEQDVPFAAGPGDQLDPAVGTDSVAYESNAAGNWDICVR
                     SISSGLERRLTTNVADQVDPAIEGSIVVWADHRNGNWDIYCRDLDSDRTSRLTTSAAD
                     QTAPQIGNGRVVYQDDRSGNWDIYAYTLASGKERRLTTDGHDQTAPQIGLGRTVVYQD
                     DRAGAADIFLCDLVTGANRAVTDDPAAQTEPCVAGAFVAWTDSRSGDPDIYGCTLAYP
                     TLTLQAPWGAPRYGSTVTFSGSLAFGAAAPAAAGISVRARAVTRTVAVSGLAGGSGDY
                     GFSLRNVVRREVVTARYAGGTAQLPSAPVSVTVVPTALLSRPVPRRIAPTSTRIWLPS
                     LDVSGTLRPKHRADSRAVRLEIWWSNLGLGWERLKTVTVRVRSVGGASVYSLKILPRM
                     NPTARYKIRAVHADSDHARTYSPFSKVVAGS"
     gene            4023..6119
                     /locus_tag="VFZ86_01040"
     CDS             4023..6119
                     /locus_tag="VFZ86_01040"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX5640902.1"
                     /translation="MVERLCRSGAPLVLVNAPAGYGKTTLLAQWVERDGRPFAWLQLD
                     RIHDDPVAFLRYLASALGNVVAVDDRIVDLLAEREPPIEEVILPGLGSAVEAAAPFLL
                     VLDDAHLVSNPACWRFADAALDQLPDGGRLALASRGEPPLPLPRRRANGDLIEILPDD
                     LRLTSEEARSVLGLHGCEAGDAELDDLLRVTEGWPTGIYLALLAGDGCPARDVLAHLR
                     GDRREIAGYLTTEVLDRQPAAVQEFLLRTSILDQLSSGLCHAVTGQANAAGLLERLAR
                     DSLFVTPLDDHDAWYRYHSLFAELLRAQLERRTPETVPDLHLRAAAWYEAHGDGERAV
                     RHAVAAGDADAVADLAALTCDVFIQAGQNERARQLLEAFSDAQLGAHPALAITAGALA
                     NYLPDARVQRWAGLSARLTLDDGPTPVGAASLRSWRAVWQANLARDGVTRMLEDATLA
                     CELEAEGRDPSWLCGAQELQCMALYLLGRVHHAQRALEATARTAAGHADEESWVLGLQ
                     TLIAADQGRWDDAVELDRQIREKTTQPKNLPPILAHALVLAHRDDPDLVAYLDKAERD
                     ARESFSPTEWRMILAATVFAGIALRRDDMAAAERWTAEAEAILRRYPDAGILRGRTRQ
                     LRKALEERRMAEPFTAAERRILDLLPTQLTAGQIAARLFLTRNTVKSHTSHIYRKLGV
                     TSRTDAVETARRLGLL"
     gene            6239..7300
                     /locus_tag="VFZ86_01045"
     CDS             6239..7300
                     /locus_tag="VFZ86_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5640903.1"
                     /translation="MRRFVIVLMLAALVLALLATAAAAGPGRACAPAPTTIATFSPGD
                     WGSFAEGMAADSHGNLYVSLTVWGYYDEEIAESNFGEVWKVVPGHDPVRLARADLSPY
                     GMLLGVAVDRCDRVYVAAYDMESGLTPNGVYRLQGDGSLTQVAALPEGVWPNGMAFHD
                     GRLYITDTALGAVWRVRLGCGVASPTAPWLQDDLLAPGDPTQDESVHGLGADGLAFRG
                     DDLFVGVYDFGRIVRVHVRDNGSPGRPTAVCERPELRTADGMAFDLAGGLWITTNGGT
                     TGASPSGALYRLAPGGRLTTVADDPGWLNYPTTPVFGRSPSARCTLFIENGAYYNWAD
                     GTSPEIQALHVGIPGLPLR"
     gene            7329..7763
                     /locus_tag="VFZ86_01050"
     CDS             7329..7763
                     /locus_tag="VFZ86_01050"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX5640904.1"
                     /translation="MELSDCTSFATEHPICFLASEDDGRPRVRPLLLWFADKRGFHFM
                     TMSPKELSEQLHRDPHVEVCFYNGAMELPEAKSMRVTGAVEFLDDAELVHQVSEERAA
                     LEGLIGRPLEPIAEVFRIASGEARFWTLGDILKERELETVRF"
     gene            7982..>8528
                     /locus_tag="VFZ86_01055"
     CDS             7982..>8528
                     /locus_tag="VFZ86_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HEX5640905.1"
                     /translation="MASRHGLVAGATGTGKTVTLQVLAEAFSRAGVPVFAADIKGDLS
                     GITQPGGGNAKVDDRVAQLGLSPWAFEGSPAMFWDVFGEQGHPVRTTASEVGPLLLAR
                     MLHLNDIQEAVLTVAFRVADDNGLLLLDLDDLRAILKFVADNAAEIGTTYGNVSAASV
                     GAIQRSLLTLEEQGGQKFFGEP"
BASE COUNT         1248 a         3204 c         2880 g         1196 t
ORIGIN      
        1 aggaggactc ctcatgctcg tcgagctcaa cgccgagcag ctggccgctg cccagcacgg
       61 cgacggcccg ctgctcatcg tcgccggcgc cggcaccggc aagacggccg cgctcgtgca
      121 ccgcgtcgcc cacctcatcg agcgcggcgc cgacccgcgg cgcatcctgc tgctcacgtt
      181 cacgcgccgc gccgccgccg agatgctgcg cagggtcgag gcggtgctcg cggcgcgcgg
      241 ccacggctcc ggcggcgcgt ccgagcgcgt ctggggcggc accttccacg ccggcgccgc
      301 ccgcctgctc cgcatccacg cgcgcgacat cggcatgcag ccggacttca cgatcatgga
      361 ccgcggcgac tccgaggacc tcatgcacgt ggcgcgcacg cgcctcggcc tgggccgcgg
      421 cggctcgcgg ttcccgcaga agtccacctg cctcgacgtg tactcgcgct gcgtcaacgc
      481 gcagctgccg ctgggcgagg tgttgcacac ggcgttcccg tggtgcctgc cggcggagga
      541 gggcctcgcc cagctcttcg ccgcgtacac ggacgccaag gaggagcagc aggtcctgga
      601 ctacgacgac ctgctgctgt tctggcgcgg cctgctcgcc gatccgggcg gcggcgcccg
      661 cgtgcgcgag cgcttcgacc acgtgctcgt cgacgagtac caggacacca acgcgctgca
      721 ggccgacatc gtgagcctgc tgcgaccgga cggcacgggc gtcacctgcg tcggcgacga
      781 cgcgcaggcc atctacgggt tccgcgccgc cacggtgcgc aacatcctcg acttcgagca
      841 gcgcttcccc ggcgccgcgg tcgtcacgct cacgcagaac taccgcagca cggcgcccat
      901 cctcgccgcc accaacgcgc tcatcgccga ggcggccgag cggcgcgaca aggagctccg
      961 caccgaccgg gccggcggcg ggcgcccggt cctcgccacc tgccgcgacg aggacgagca
     1021 gacgcagtgg gtctgcgagc acatcctcgc gcaccgcgag cagggcacgc agctcaagga
     1081 gcaggccgtg ctgtaccgcg cgcagcacca cagcatggcg ctcgagatgg agctcagccg
     1141 ccgcaacatc cccttccaca agtacggcgg gctgcgcttc gtcgagatgg cccacgtcaa
     1201 ggacctcgtg agcttcctgc gcctggccga gaaccccaaa gacgcgatgg ccggcctgcg
     1261 cgtgctcggc ctcgtgccgg gcatcggccc gaagacggcg gcggcgctgc tcgaggcgct
     1321 gggcgctgcc gccggcgact tcgcggcggc ctggaccggc gcgcgcgtcc ccgagccggc
     1381 gcgcgccgcc tggccggccg tcgtcgagct cttcgcgacg ctcgccgccg ccggcccgtc
     1441 ggaggtcccc gcgcaggtcc acgccgtgcg cagcgtgtac ggaccgctgc tcgagcgtcg
     1501 ctacgacaac gtccaggcgc ggctgcgcga cctcgagcag atcgagggac tcgcggcccg
     1561 ctcgcagacg cgctcgcagt tcctcgccga gctggtgctc gacccgccgg cgtacacgca
     1621 ggagctggcc ggcccgccgc tgctcgacga ggactacctc atcctcagca ccatgcactc
     1681 cgccaagggc ctcgagttcg acgtcgtcta cgtcatccac gccgccgacg gcaacatccc
     1741 gtcggacatg gccacgggca gcgccgccga gatcgaggag gagcggcgcc tgctctacgt
     1801 cgcctgcacg cgcgcccgcg agcagctcta cgtgagccac ccgctgcgct actacacgca
     1861 gccgtgggcc aaggccgaca cgcacgggta cgcgcagcgc tcgcgcttcc tcacgcacgc
     1921 cgtgacggcc cacttcgacg aggtccagac ggggccggaa cacgcgccgt cggaagaagg
     1981 cccgctcggc cgggtcacga ccgcgtccat ccgcgccggc gtgcgctcgc tgtgggaggc
     2041 gccggccgca ggctgacaga gctgcagtca gcccgattcc cgcagaccaa cgaatcaggt
     2101 aggatgcggc ggaccggggc aagtactgga ggggtccaat gccgcgcctg ttcgccacgt
     2161 tcgtctgctc gtctctcctc atcatcgtca tgctcgcgtc gcctgcggga gcctcacccc
     2221 ccttcgccca ggagacaccc atcgccgcgg gcccgggcgc gcaacaatca ccggacctcg
     2281 gcccctccct gtggaactgg ccgctgacct gggagaccga tgcatccggc gtgtggcacg
     2341 caggatggcg ggactccacg cagaccgaga ccgtctcaac cgccggcggg gcgcagcgtc
     2401 acccggcggt cttccaggac cgcctggtct acgaggacga ccgcaacggc accgtcgatc
     2461 tctaccgcac gtggctcgcg ctggccggct accccacccc caccgagcag gacgtgccgt
     2521 tcgccgcggg gccgggcgac caactggacc ccgccgtggg cactgactcg gtggcctacg
     2581 agagcaatgc ggccggcaac tgggacatct gcgtacgctc catctcgagc ggactggagc
     2641 gcaggctcac caccaacgtc gcggaccaag tggacccagc catagagggc tccatcgtcg
     2701 tgtgggcgga ccaccgcaac ggcaactggg acatctactg ccgcgacctg gacagcgacc
     2761 ggacctcgcg cctgaccacc agcgccgcgg atcagacggc tccgcagatc ggcaacggcc
     2821 gggtcgtcta tcaggacgat cgcagcggga actgggacat ctatgcgtac acgctcgcca
     2881 gcgggaagga acggcgattg acgactgacg ggcacgatca gaccgctccg cagatcggcc
     2941 tcggacggac cgtggtgtat caggacgatc gggcgggcgc agcggacatc ttcctgtgcg
     3001 acctggtcac cggcgcgaac agggcggtca ccgacgaccc tgcggctcag actgagccgt
     3061 gcgtcgccgg cgccttcgtc gcgtggaccg attcccgctc gggcgacccg gacatctatg
     3121 gctgcacgct ggcctacccc acgctcacgc tccaagcccc ctggggcgcg ccgcggtatg
     3181 gctccacggt gaccttttcg ggatctcttg ccttcggcgc cgcggcaccc gccgcagccg
     3241 gcatctccgt ccgtgcccgc gccgtaacgc gcaccgtcgc ggtgtcgggg ctcgcaggcg
     3301 ggtcgggcga ctacgggttc tcgctccgga acgtcgtcag aagagaggtc gtgaccgcgc
     3361 gctacgccgg ggggaccgcg cagctcccgt ccgccccggt cagtgtgacc gtcgttccga
     3421 cggccctgtt gagccgcccg gtccccagga ggatcgcgcc gacctcgaca cgcatctggt
     3481 tgccctctct cgatgtatca ggaacgctca gacccaagca cagggcggat tcgagggcgg
     3541 tccgcctcga gatctggtgg agcaacctgg ggctgggctg ggagcgcctc aagaccgtca
     3601 cggtccgggt gcgcagcgtc ggcggcgcca gtgtctactc gctcaagatc ctgccgagga
     3661 tgaacccgac cgctcgatac aagatccgcg ccgttcacgc ggactcggac cacgcgcgca
     3721 cgtactcccc attcagcaag gtcgtcgccg ggtcctgacg acggacgccc ctgcccagaa
     3781 gcatgcggcg cgcggattct cctcaggttg cgacgatgcg gcaacggacc ggtgatcgcc
     3841 aacactgctc cacccatcat cgcggtgcta cgatgcacgc cggagggaga gagggggcat
     3901 caccggtggg gcgtgacgcg cgtgcaaccg gggacaacgg gccgcagccg gagcgattcc
     3961 ggccgctcgg gtctcgtctg cgccctccgc gcgcccacct caagctggtg gtgagagagg
     4021 ccttggtaga gcggctctgc cgctccggcg ccccactcgt gctcgtcaac gcccccgccg
     4081 ggtacggcaa gaccaccctg ctcgcacagt gggtcgagcg cgacggccgg ccgttcgcct
     4141 ggctccagct cgacaggatc cacgatgacc cggtcgcgtt tctccgctac ctggcgtccg
     4201 cgctcggcaa cgtcgtggca gtcgacgacc ggatcgtcga cttgctggcc gagcgagaac
     4261 ccccgatcga agaggtcatc ctccccgggc tcggatctgc cgtggaggca gccgccccct
     4321 tcctgctcgt tctcgacgac gcccacctgg tcagcaaccc cgcgtgctgg cgcttcgccg
     4381 acgcggcgct cgaccagctc cccgacgggg ggcgcctggc gctggcctcc cgcggcgagc
     4441 cgccgctgcc gttgccacgc cggcgcgcga acggagacct gatcgagatc ctccccgacg
     4501 acctgcgttt gacctcggag gaagctcgct ccgtcctcgg cctccacggc tgtgaagccg
     4561 gtgacgccga actggacgat ctgcttcgcg tcaccgaggg atggccgacc gggatctacc
     4621 tcgccctcct ggccggcgac ggctgcccgg ccagggacgt cctcgcgcac ctgcgcggcg
     4681 accggcgtga gatcgccggg tacctcacca cggaggtgct cgaccgccag cccgccgccg
     4741 tgcaggagtt cctgctccgt acctcgatcc tcgaccagct ctcgtcgggc ctgtgtcacg
     4801 cggtgaccgg ccaggccaat gccgccggcc tgctcgagcg gctggctcgt gacagcctct
     4861 tcgtgacccc actcgacgac cacgacgcct ggtatcgcta tcactccctg ttcgccgagc
     4921 tgctgcgcgc tcagctcgag cgacggacgc cggagaccgt gccggacctc cacctgcgcg
     4981 ccgccgcctg gtacgaggca cacggcgacg gcgagcgcgc ggtgcgccac gccgtcgccg
     5041 ccggtgacgc cgatgccgtc gccgacctgg ctgcgctgac ctgcgacgtc ttcatccagg
     5101 ccgggcagaa cgaacgcgcc cgtcagctgc tcgaagcctt ctccgacgcg caactcggcg
     5161 cccacccggc cctggcgatc accgccgggg cgctcgcgaa ctacctgccg gacgcccgcg
     5221 tgcagcgctg ggctggcctg tccgcgcggc tcacactgga cgacggcccc acacccgtgg
     5281 gcgccgcttc actgcgctcg tggcgagccg tgtggcaggc gaatctcgca cgcgacggcg
     5341 tcactcgcat gctcgaggac gcgacgctgg cgtgcgagct cgaggcggaa ggccgagatc
     5401 cgtcgtggct ctgcggcgct caggagctcc agtgtatggc gctgtacctc ctggggcgcg
     5461 tgcaccacgc tcagcgcgcc ctcgaggcga cggcgcggac cgcggcgggc cacgcggacg
     5521 aagaatcctg ggtcctcggc ctgcagacgc tcatcgccgc cgaccaggga cgttgggacg
     5581 acgccgtcga gctcgaccgc cagatccgcg agaagacgac gcaacccaag aacctgccgc
     5641 cgatcctcgc gcacgcgctc gtcctggctc atcgcgacga ccccgatctg gtcgcctacc
     5701 tggacaaggc ggagcgcgac gcgcgcgagt ccttctcgcc caccgagtgg aggatgatcc
     5761 ttgccgccac cgtcttcgcc gggatcgcgc tgcggcgcga cgacatggcg gccgcggaac
     5821 gctggaccgc ggaggccgag gcgatcctcc ggcgctatcc ggacgcggga atactgcggg
     5881 gccgcacgag gcaactccgc aaggcgctcg aggagcgccg catggcggag ccgttcaccg
     5941 ccgcggagcg ccgcatcctc gacctgctgc cgacccagct gaccgccgga caaatcgccg
     6001 cccgtctctt cctgaccagg aacacggtca agtcgcacac gagccacatc taccgcaagc
     6061 tcggtgtcac gtcccgcacg gacgcggtcg agacggcgcg ccggctcggg ctgctctgac
     6121 ggtcccggtc gctcgaagca cggttcctcc ccaggtgact tcacaccttt ggggtgacga
     6181 cgcagtcgag gcctcctgcg acgatgcgca cgacatcgtc gtaaatgaag gaggactcat
     6241 gagacgcttc gtcatcgttc tgatgctggc ggcgctcgtg ctcgcgctgc tcgcgacggc
     6301 cgccgcggcc ggccccggcc gcgcctgcgc gccggcgccg accacgatcg ccaccttctc
     6361 gccgggcgac tggggttcgt tcgccgaggg catggccgcg gacagtcacg gcaacctgta
     6421 tgtctcgctg accgtctggg gctactacga cgaggagatc gccgagtcga acttcggtga
     6481 ggtgtggaag gtggtgcccg gtcatgatcc ggtccgtctg gcccgggcgg acctctcgcc
     6541 ctacgggatg ctgctcggcg tcgccgtcga ccgctgcgat cgcgtgtacg tcgccgccta
     6601 cgacatggag tcgggactca cccccaacgg cgtctatcgc ctgcagggcg acgggagcct
     6661 gacgcaggtc gcggcgctcc cggaaggcgt gtggcccaac gggatggcct tccacgacgg
     6721 gcggctctac atcacggaca cggcactcgg cgcagtatgg cgggtgcgtc tcgggtgcgg
     6781 cgtcgcttcg ccgaccgcgc cgtggctgca ggacgacctg ctggctccgg gtgatcccac
     6841 ccaggacgag agcgtgcatg ggctcggcgc ggacggcctc gccttccgcg gcgacgacct
     6901 gttcgtgggc gtctacgact tcggtcggat cgtgcgcgtc cacgtccgcg acaacggttc
     6961 gccgggccgg cccaccgccg tctgcgagcg tcccgagctc cggacggccg acggcatggc
     7021 cttcgacctc gccggcggtc tgtggatcac gaccaacggc ggcaccaccg gcgcgagccc
     7081 cagcggtgcg ctgtaccgtc ttgcgcccgg cggccggctc accacggtcg ccgacgaccc
     7141 cggctggctg aactacccga cgacgccggt cttcgggagg tccccgtcag cccggtgcac
     7201 gctgttcatc gagaacggcg cctactacaa ctgggcggac ggcacgtcgc cggaaatcca
     7261 ggccctgcac gtcggcatcc ccggcctgcc gctgcggtga cgcggcccgg cgacgtgaag
     7321 gaggggtgat ggaactcagc gactgcacca gcttcgcgac cgagcatccg atctgtttcc
     7381 tcgcgagcga ggacgacggc cgaccgcggg tccgtccgct gctcctctgg ttcgcggaca
     7441 agcgcggctt ccacttcatg accatgagcc cgaaggagct ctcggagcag ctgcaccggg
     7501 acccgcacgt cgaggtctgc ttctacaacg gagccatgga gctgcccgag gcgaagtcga
     7561 tgcgcgtgac cggcgcggtc gagttcctcg acgacgccga gctcgtgcac caggtgagcg
     7621 aggagcgggc ggcgctcgaa gggctcatcg gccgcccgct cgagccgatc gccgaggtgt
     7681 tccgtatcgc ctccggcgag gcccggttct ggaccctcgg cgacatcctg aaggagcggg
     7741 agctcgagac ggtccgtttc tgaggccgga agaacgcggt ggccgggtgg ccggaggcgg
     7801 cgaagcgccg cctccggcca ccgtcgcgtc tgcctcgcca cttctccgac gggtgaaccc
     7861 ggccgccggg ggtatgctcc tgcgcgagat ctctcccccg gggaaggagc gtgacgtgtc
     7921 cgactcatcc gcaggcctgc tgatcgccaa gaacgccgcc gagctgcagt tgctgccgca
     7981 gatggccagc cggcacggcc tcgtggccgg cgccaccggc accggcaaga cggtgaccct
     8041 gcaggtgctg gccgaggcgt tcagccgcgc cggcgtgccc gtcttcgccg ccgacatcaa
     8101 gggcgacctc tcgggcatca cccagccggg cggcggcaac gccaaggtcg acgaccgcgt
     8161 cgcgcagctc ggcctctccc cctgggcgtt cgaaggctcg ccggcgatgt tctgggacgt
     8221 gttcggcgag cagggccacc ccgtgcgcac caccgcgtcc gaggtcgggc cgctgctgct
     8281 cgcgcgcatg ctccacctca acgacatcca ggaggccgtg ctcacggtcg ccttccgcgt
     8341 ggccgacgac aacggcctcc tgctgctcga cctcgacgac ctgcgcgcca tcctcaagtt
     8401 cgtcgccgac aacgcggccg agatcggcac cacctacggc aacgtctccg ccgcctccgt
     8461 cggcgcgatc cagcgttcgc tgctcacgct ggaggagcag ggcgggcaga agttcttcgg
     8521 cgagcccg
//
LOCUS       DASUMX010000023        18072 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_149585, whole genome shotgun sequence.
ACCESSION   DASUMX010000023 DASUMX010000000
VERSION     DASUMX010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 18072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18072
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149585"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1086
                     /locus_tag="VFZ86_01060"
     CDS             <1..1086
                     /locus_tag="VFZ86_01060"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HEX5640906.1"
                     /translation="ATAQHTTVVAPEVDRRVGPHRISVKTALIVLLGVLAVMLVAVPA
                     VSATGSVTKAKARLRAVEARLDDVATQSEIAVEKYNQASTQLETVQAKIDENQRQLEV
                     ARQNLAQASEQLVLRAQNIYKARDVGVIDVIFQADSFDDLISQMDMMERLGNSDVDTV
                     HAIAAYKRDIKDRRVRLEADEKAAARLVAEREQHKDELLALEADLQDLTGKIERQIRK
                     LRAEARRRARIALTGFSGPVPVVDPNSPGHPEIVAIAQRYFGVPYVWGGASPSGFDCS
                     GLTMYCYASVGIGLYHGATMQQRASTPVALTDLRPGDLIFFGNASFSHHVAIFVGGTT
                     CIEAPRTGDVVRYGTWTGRDAWIGGRF"
     gene            1209..2300
                     /locus_tag="VFZ86_01065"
     CDS             1209..2300
                     /locus_tag="VFZ86_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013006797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEX5640907.1"
                     /translation="MKPIDDLLSEMMARNASDLHIKAGSPPVIRVDGELALLDEPALT
                     PEDTKDVAASIMTDKQIRRFSEHNEIDFAYSAATLGRFRVNVFRQRGSISVAMRQVTT
                     KIPSFEELHLPDIIKRLALEPRGLVLVTGTTGSGKTTTLAAMIDHINDTMRRHIVTVE
                     DPIEVLHKDKKSIINQREIGLDTDSYATALKYVLRQDPDDILIGEMRDPETVSAALTA
                     AQTGHFVMSTLHTIDATETINRIIDFFPLHQQKQIRIMLAGTLKGIVSQRLLVRADGE
                     GRVPAIEIMVCTNRIRDFILDPDQAGLIGDAIKEGDFYGMQTFDQALLTLYEDGLITL
                     ADAAQVASNPHDFKLLVQAQGHDVSLMTF"
     gene            complement(2405..3082)
                     /gene="sigH"
                     /locus_tag="VFZ86_01070"
     CDS             complement(2405..3082)
                     /gene="sigH"
                     /locus_tag="VFZ86_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HEX5640908.1"
                     /translation="MARQADHRRGSGSSHNPQVFNESSDFALVQAYRRGNQAALDALM
                     IRYHQFVRLKASSYFLAGGDAEDLIQEGMLGLYKAVRDYRDDRLASFRSFAELCITRQ
                     IITAIKTATRQKHVPLNGYLSFSQSPSDSSDDGDCSLADVLPGPTTHDPISQVISSEE
                     VDSLTNCLTSMLSELEGKVLRFYLQGLSYERIAGELGCDCKTVDNALQRIKRKVDLHL
                     EGRRVPT"
     gene            complement(3147..3662)
                     /locus_tag="VFZ86_01075"
     CDS             complement(3147..3662)
                     /locus_tag="VFZ86_01075"
                     /inference="COORDINATES: protein motif:HMM:NF017774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEX5640909.1"
                     /translation="MSGTLYIVDGYNVLHALFRGAEKEEIFARRDWLADSLASFVALR
                     GTRAVLVFDGTGPRSTSSEPIRGAAVEVVFAGGRFTADTLIARRIAGQPADVQVVVVS
                     ADQEVQRTASRAGVSRMTPRELGAELRGGGESGQALDSAPDSTRMPSRLEDKVDPETL
                     RRLEELRHKPQ"
     gene            complement(3659..4390)
                     /gene="rlmB"
                     /locus_tag="VFZ86_01080"
     CDS             complement(3659..4390)
                     /gene="rlmB"
                     /locus_tag="VFZ86_01080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEX5640910.1"
                     /translation="MEWLYGRNVVKAALGAGARRRAHKLAATPPALDAVAGLVPEDLP
                     VDTVSAHQLDELLGTREHQGLALQVDGYPYADPDRLLKADVLVVLDEVTDPHNLGAVA
                     RTALAAGAGGLVVPRHRSAHVTPAAVKASAGTLELLAVAQVTNVVAFLHRARDAGFWV
                     YGAAGEAAASYLDTDLTGRIVLVAGAEGRGLRPLVARTCDALLSIPMEPPVESLNVSV
                     AAALLLYEARRQREAAGKGPAGGGR"
     gene            4451..4840
                     /locus_tag="VFZ86_01085"
     CDS             4451..4840
                     /locus_tag="VFZ86_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5640911.1"
                     /translation="MPIDFSPGSFAATAIAVLFWWTVLSPAALLAIAALCGRRPSLRG
                     VLTLVVLVPPVVTLLPVAVMHGGLAAAAAAAGSLVLGAAIVLTIVGLSRRSDPARAER
                     YASAALLAGVAGGMIGAGALLGARLFG"
     gene            complement(4885..6432)
                     /gene="cysS"
                     /locus_tag="VFZ86_01090"
     CDS             complement(4885..6432)
                     /gene="cysS"
                     /locus_tag="VFZ86_01090"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009306414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEX5640912.1"
                     /translation="MRLFNSLTQQKEPFEPRDPGKAGIYCCGPTVYNLVHVGNARPYV
                     TFAVLRSWLAYRGLEVTLVTNITDVDDKIITKANAEGRDSAAVAQEYTAAYVADTDRL
                     GIPRPDVEPKATETIPEIVDLVCQLISKGHAYEAGGSVYFKVRSFEQYGKLSKQRIDE
                     LEEGVRVDAEPGKADPLDFAVWKAAKPGEPAWESPWGLGRPGWHIECSAMGLRYLGAG
                     FDVHGGGRDLIFPHHENEIAQSEAAGLPFAKVWMHNGMIRSEGEKMAKSVGNIFLLRE
                     VLDRYEPAVVLTYFLTTHYRSPLEFSAEKLDEAKAAYGRFASALADIDFRVVNASRAA
                     ARGEWPDLTLRAEAARVAFGERMDDDLNTAGAIGELFGLVGEMHRYLTLVDRGEAPLD
                     TGALTAVGDVLRASLTVLLIPVAGAGAGLTAVGSVAGDDAAACATGGVALLPAARLAE
                     EGRWDDVALVYADRLGCDDASYACVLRDHYRAGKQWADADRLRDEIQAAGFEVRDTPQ
                     GTQVVKR"
     gene            complement(6432..7886)
                     /gene="gltX"
                     /locus_tag="VFZ86_01095"
     CDS             complement(6432..7886)
                     /gene="gltX"
                     /locus_tag="VFZ86_01095"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEX5640913.1"
                     /translation="MRVRFAPSPTGTLHIGSARTALYNFLFARHNGGSFVLRVDDTDA
                     ARSDAALEESIMADLRWLGLAWDEGPDLGGPAGPYRQSERLEGHLEAAARLLDAGLAY
                     RCFCPEERLEELRREALAAGRTPRYDRRCLGLPAAEVRRRLDAGEPAAVRFRVPAGDV
                     VIDDRIRGQVVIGADALSDPIIVRSDGVAGYNFATVVDDLAMGITHVIRGDDHLTNTA
                     RQQLLFDALGGPVPRFAHHSMVLGPDGGKLSKRHGATAVGDYRELGYLPEAIVNYLAL
                     LGWSHGEDEVLDLERLIGEFELDSLSASPAVFDLAKLDWLDHERIMALAPEEHERRFA
                     ERLPAGTPPQAAAALAAAFQPSLVRYGEAPGAAAEVLAPPPPPDELRAVALAAAPQLA
                     QVRALRAEAPEWLTPAGARDLLAGYRAWGKQRSIGARDLLMPLRIALTSAEHGPELPF
                     VLAALSRGTALARLDLILAGNVAGESTPTPGEAP"
     gene            8028..9500
                     /locus_tag="VFZ86_01100"
     CDS             8028..9500
                     /locus_tag="VFZ86_01100"
                     /inference="COORDINATES: protein motif:HMM:NF013444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX5640914.1"
                     /translation="MPHDQRETPYLDAALRYKATGYTPFHTPGHKLGKGAPAGLREFV
                     GDACLSADLAMAGAVEDTRESTGLIRIAEGYAAEAWGSDRTWFLVNGSSSGVHALLLT
                     LCGPGDTVIVPRNAHKSMLAGLIFTGATPLYMEPAIDQLWGIPLQVRAADAQRALERA
                     PDARALFVTSPTYNGFGADLQGIGALAHAAGIPFVTDQAWGPHLRFCPELPVDAMTAG
                     ADAAVVSTHKLISGITQSAVLMARGERVNLARLDAMVHLTQSTSPQVLMYASIDAARA
                     QMAVQGAELWRGAIELAQWARAEISRMRGLRCLGAEMLGREGVSEFDPTRLTVSACDL
                     GLSGYQLETLLRDDYRIAVEAADPLNIVLNVTFGDSRADLETLVAALADLAARYAGAA
                     AGAAAACSTLLAHTPPFTRQVLSPRDAVFAPAVALPLGDCAGQVSAEMVTPYPPGIPV
                     LGPGEEISPEIVAYLREAGDAGLKVHGPEDRTLRSLRVVA"
     gene            complement(9526..10059)
                     /gene="pyrE"
                     /locus_tag="VFZ86_01105"
     CDS             complement(9526..10059)
                     /gene="pyrE"
                     /locus_tag="VFZ86_01105"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014438339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEX5640915.1"
                     /translation="MTTDLGQRLLDASYLEGDFLLRSGRRSKFYLDKYLFSTRPDLLR
                     DIAAGIAGKLPAYEPFDLLAGPELGAVAIVAAVSLASGKPFVIVRKGAKGYGTDKAME
                     GRAEPGQRVVMVEDVVTSGGAALMAADKLREAGLEVSTLICVVDREEGGRAQIEEKGL
                     TFDPLFTATSLGIKKPE"
     gene            complement(10178..11518)
                     /locus_tag="VFZ86_01110"
     CDS             complement(10178..11518)
                     /locus_tag="VFZ86_01110"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEX5640916.1"
                     /translation="MFEILDAVLERAQARGAADVEVFGERSTSHRIKVYRQEVEQLTA
                     AQRRGVGVRVFSGGAVGHAYTSDLSDGALDDVVQRAVDNAAVSDPDEFAAVPQPGGEP
                     ADVHPYDPRLAAATDEQRIAVALEVEATALAADPRVKTVEDTMYVDGEGEVFIASTAG
                     VRGSFRAGQCYAFAYVLAEEQGQVETGYSYSVGRALEDLDAAAVGTEAAGRATRLLGA
                     RKCPSMKAPVILDPFVSAAFFGVLSSALTADAVQKGRSLFAGREGERVAGELLELVDD
                     GTVEDGLDSAPFDGEGVPCGRTPLITGGVLQGFLYDTYTARKAGRESTGNGMRGGYSS
                     LPGARPSNLLVTGPATPLDDIVAGMQKGVLVTDAVGIHSGANPISGEFSVGISGVLIE
                     DGAFTTPVREVTLAGDIVGMLTSIRALGDDARWVPGGSIFTPSVVIDGMAIGGT"
     gene            complement(11518..12918)
                     /locus_tag="VFZ86_01115"
     CDS             complement(11518..12918)
                     /locus_tag="VFZ86_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEX5640917.1"
                     /translation="MDVASFDTALLRATMERALRRGGDFAEVFVEDRETLGLRLEDGR
                     IEQTSGGRETGAAVRLLSGERTYYAYSDAVDEAGLTAAADAVAAAVSAGARGPEIVDL
                     GAVLADPGKNPVLTPPGEVAIARKAVLVRAGDEAARAASSEIVQVIVGYGDTRQKVLI
                     ANSLGDLVFDDRTRTRFSAQVVARRGEVIQTGHETVGGSAGFELVDEEIARGVAQTAA
                     DKARIMLDSKPAPVGAMPVVLANGFGGTLFHEACGHGLEADAIAKGASIYEGKLGEVV
                     AASIVSAYDEGSITNGWGSQAYDDEGVPTQKTLVIEEGRLTGYLYDRLRAREAGAAPT
                     GNGRRQSFRHVPIPRMTTTYIAPGDATAEEIIAATPRGFYAKSLAGGQVEPASGNFVF
                     GVAEGYLIEDGKVTTPLRGATLVGNGIDILMKIDLIAGDFDVKTGICGKDGQGVPVGT
                     GQATLRIAEMTVGGTG"
     gene            complement(12918..13133)
                     /locus_tag="VFZ86_01120"
     CDS             complement(12918..13133)
                     /locus_tag="VFZ86_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5640918.1"
                     /translation="MRARTVALAALGAVTVAGLVWWRRRTPAPADLLVQFGARDGAVR
                     TLDLFDPSTAELETLAAGVRDSLTGGA"
     gene            complement(13130..13450)
                     /locus_tag="VFZ86_01125"
     CDS             complement(13130..13450)
                     /locus_tag="VFZ86_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5640919.1"
                     /translation="MSTDITVVAVIDASGSVVACAPDAAGAGFAEAAEALWSAASRRA
                     AAAGTAGLEHVVVPAGDAVVAVVEAHGHRAVAVTGPRPAVGLLLFDLRTCLGDAFAAA
                     EGAR"
     gene            complement(13539..14246)
                     /locus_tag="VFZ86_01130"
     CDS             complement(13539..14246)
                     /locus_tag="VFZ86_01130"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5640920.1"
                     /translation="MDRDHELSAVLADDTRYHIYRWIAERPGTDVTVADVSAEFGLHP
                     NVARMHLAKLEQAGFLATGFRRAAGGGRPAKLYRLSDKVVTFGFPPRRYELLSRLALD
                     ALAAGGTRDDALRVCREAGAAEGRRALAGDAEPPRDRAAVAGLVATIAEEQGLLPEVD
                     WLDGALFIVVNNCSFRELSGADPDLVCAMHRAFLEGILQVSTAGLGPLRIEPGDCRIS
                     CGGERCELLCSFASGQE"
     gene            complement(14333..14749)
                     /locus_tag="VFZ86_01135"
     CDS             complement(14333..14749)
                     /locus_tag="VFZ86_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005490366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX5640921.1"
                     /translation="MARSTPVSVDWKREGLHFEATTPHGTIALASALDEPGNGVTPME
                     TLATALGACTAMDVASILVKMRQPLEGFRVEVSGERAEEHPKKFLSLDVVYHLKGDLD
                     ERKVERAIELSEERYCSVAATLKPALPITSSYVIER"
     gene            14869..15717
                     /gene="uppP"
                     /locus_tag="VFZ86_01140"
     CDS             14869..15717
                     /gene="uppP"
                     /locus_tag="VFZ86_01140"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006715669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphatase UppP"
                     /protein_id="HEX5640922.1"
                     /translation="MTILQAIILGIVQGLTEFLPISSSGHLLLVPWLFNWHFLLENPE
                     LNKTFDVALHLGTFVAVVLYFWREIVRLVGAWVRSLSRRSLAEPEAKLAWLLIVSTVP
                     AALVGVLFEDFITNTLGKPWMIGVAMIVFAGFMYLVDHIARLDRDLEALTWLDALLIG
                     IAQSLALLPGVSRSGITMMTGLLVRLDRESAARYSFLMSIPVIGGAAAFKGLEVARDG
                     LPAGTATPFAVGMVAAALSGIAAIWFTLAYLKRHNFNLFVVYRIVIGVGVLVLIVAGV
                     RSGMAV"
     gene            15823..16911
                     /locus_tag="VFZ86_01145"
     CDS             15823..16911
                     /locus_tag="VFZ86_01145"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX5640923.1"
                     /translation="MRDLSRGDRGKEVLDVQTRLRGQGFELGREGVDGFFGPHTEAAV
                     LSFQQERGLLADGRVGANTWRELVESGYALGDRLLYLREPPFRGDDVLRLQVKLNLLG
                     FNAGPERGVHDDEVERAVLDFQRNAGLPVDGIVGESTLRKLEAVRKAESGREGKKIPE
                     RDRGYVAARGLTGQSVTIDPGHGGADSGGVSARGLAEKDVTLALGLRLAELLRAEGCR
                     VQLTREGDERVPLYARADAANGAASAFFLSLHCNGDSSPAARGAAVYYFQRSHYYSEH
                     GRRLAGSIGARMRAAGVPWIGRFGRNYALLREARGIALLVEPLFLTSPEDETLARRPE
                     HVERLARALAGGLADYLARVPLEGEEAS"
     gene            16908..17768
                     /gene="pylSc"
                     /locus_tag="VFZ86_01150"
     CDS             16908..17768
                     /gene="pylSc"
                     /locus_tag="VFZ86_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014184084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043767 - pyrrolysyl-tRNA synthetase
                     activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrrolysine--tRNA(Pyl) ligase large subunit"
                     /protein_id="HEX5640924.1"
                     /translation="MTPDEADVLRIRLTATQSQRLRELGADPLVLDELFADASARDAA
                     FKALEGELVRDGRRRLRELREGRRAPQLVELERDLSDALTGAGFVRVVTPLIIASDAL
                     RKMGIEHGHPLREQVFWLEDGRCLRPMLAPNLYTLLRRLDRHWSKPFGIFEIGPCFRR
                     DSKGASHLNEFTMLNLVELGRPESEGRARLAELASAVMRAAGVSHYEFEVTESEVYGE
                     MVDVLVNGAEVCSAGLGPHPLDGNWGIVDPWVGLGFGLERLIMAREGYPNIERAGRSL
                     SYVDGVRLNL"
BASE COUNT         2463 a         6642 c         6395 g         2572 t
ORIGIN      
        1 gcgaccgcgc agcacaccac agtcgtggcg ccggaggtag accgccgagt gggaccacac
       61 cgcatcagtg tcaagaccgc gctcatcgtc ctcctcggcg tgctcgcggt catgctcgtc
      121 gccgtgcccg cggtgtcggc aacgggctcc gtcaccaagg ccaaggcgcg cctgcgcgcc
      181 gtggaggcgc gcctcgacga cgtcgcgacg cagtccgaga tcgccgtgga gaagtacaac
      241 caggcctcca cccagctcga gacggtgcag gccaagatcg acgagaacca gcgccagctc
      301 gaggtcgcgc gccagaacct cgcgcaggcg agcgagcagc tcgtcctgcg cgcccagaac
      361 atctacaagg cgcgcgacgt cggcgtcatc gacgtgatct tccaggccga cagcttcgac
      421 gatctcatca gccagatgga catgatggag cggctcggca acagcgacgt ggacaccgtc
      481 cacgccatcg ccgcctacaa gcgcgacatc aaggaccgcc gcgtgaggct cgaggcagac
      541 gagaaggcag ccgcccgtct cgtcgccgag cgcgagcagc acaaggacga actgctcgca
      601 ctggaggcgg acctccagga cctcaccggc aagatcgaga gacagatcag gaagctcagg
      661 gcggaggccc ggcggcgtgc ccggatcgcg ctcaccggct tctccggccc cgtccccgtg
      721 gtcgacccca acagccccgg ccacccggag atcgtcgcca tcgcgcagcg ctacttcggc
      781 gtgccgtacg tgtggggcgg cgccagcccg agcggcttcg actgcagcgg cctcaccatg
      841 tactgctacg cgagcgtcgg gatcggcctg taccacggcg cgaccatgca gcaacgcgcc
      901 agcaccccgg tcgcgctcac cgacctgcgc cccggcgacc tcatcttctt cggcaacgcc
      961 agcttcagcc accacgtcgc gatcttcgtc ggcggcacca cctgcatcga ggcgccgcgc
     1021 accggcgacg tggtgcgcta cggcacctgg acgggtcgcg acgcgtggat cggcggccgc
     1081 ttctagccac ccggctcgcc gccgctccac gctcggcacg ggacatgtgc ggggtccgga
     1141 ggtaagcttt tccgcggttc tgccgaacaa ggacacggag agagaacccg agcgactggg
     1201 gctgcgcgat gaaaccgatc gatgatctgc tcagcgagat gatggcgcgc aacgcgtcgg
     1261 acctgcacat caaggcgggg agcccgcccg tgatccgcgt cgacggcgaa ctggcgctgc
     1321 tcgacgagcc ggccctcacc cccgaggaca ccaaggacgt cgcggcgtcc atcatgaccg
     1381 acaagcagat ccgccgcttc agcgagcaca acgagatcga cttcgcctac tcggcggcga
     1441 ccctcggccg cttccgcgtc aatgtgttcc gccagcgcgg cagcatcagc gtggccatgc
     1501 gccaggtcac caccaagatc ccttccttcg aggagctcca cctcccggac atcatcaagc
     1561 gcctcgcgct cgagccgcgc ggcctggtgc tcgtcaccgg cacgaccggc tccggcaaga
     1621 ccaccaccct cgcggcgatg atcgaccaca tcaacgacac gatgcgccgg cacatcgtca
     1681 ccgtcgagga ccccatcgag gtgctgcaca aggacaagaa gtcgatcatc aaccagcgcg
     1741 agatcggcct cgacaccgac agctacgcaa cggcgctcaa gtacgtgctg cgccaggacc
     1801 ccgacgacat cctcatcggc gagatgcgcg acccggagac cgtgtccgcc gcgctcacgg
     1861 ccgcgcagac cggccacttc gtgatgagca cgctgcacac catcgacgcc accgagacga
     1921 tcaaccgcat catcgacttc ttcccgctgc accagcagaa gcagatccgc atcatgctgg
     1981 ccggcacgct caagggcatc gtcagccagc gtctgctcgt gcgcgccgac ggcgagggcc
     2041 gcgtgcccgc catcgagatc atggtgtgca ccaaccgcat ccgtgacttc atcctcgacc
     2101 cggaccaggc gggcctcatc ggcgacgcga tcaaggaggg tgacttctac gggatgcaga
     2161 cgttcgacca ggccctgctc accctgtacg aggacgggct catcacgctg gccgacgcgg
     2221 cgcaggtggc cagcaacccg cacgacttca aactcctggt gcaggcgcag gggcacgacg
     2281 tgagcctcat gacgttctga ggcgcggcgg gtggacgcga aacgggcggg gcggcctcat
     2341 ccggccgccc cgcccgtttc gtctccgcgc gcgccggctc cgctgaagcg gagcggccga
     2401 cgcgtcaggt cgggacgcgt ctcccctcga ggtgcaggtc gaccttgcgc ttgatgcgct
     2461 gcagcgcgtt gtcgaccgtc ttgcagtcac agccgagctc gccggcgatg cgctcgtagc
     2521 tcagcccctg caggtagaag cgcagcacct tgccctcgag ctcggagagc atgctcgtga
     2581 ggcagttcgt gaggctgtcg acctcctcgg agctgatcac ctggctgatc ggatcgtgcg
     2641 tggtggggcc gggcagcacg tcggccagtg agcagtcgcc gtcgtccgac gagtcgctgg
     2701 gcgactggct gaacgagagg tagccgttga gcggcacgtg cttctgcctc gtggccgtct
     2761 tgatggcggt gatgatctgg cgcgtgatgc acagctcggc gaagctgcgg aagctggcca
     2821 ggcgatcgtc gcggtagtcg cggacggcct tgtagaggcc gagcatgccc tcctggatga
     2881 ggtcctccgc gtccccgccg gccaggaagt acgaactggc cttgaggcgc acgaactggt
     2941 ggtagcggat catgagcgcg tcgagcgccg cctgattgcc gcgccgatac gcctggacga
     3001 gcgcgaagtc agaactctcg ttgaagactt gagggttgtg agacgagccc gagccacgtc
     3061 tgtggtccgc ttggcgagcc atgcgtgatc cccacctccg actcgtttgc ccctgcacat
     3121 cccggataat cccccctccg gaacgtctac tgcggcttgt ggcgcagctc ctcgagacga
     3181 cgcaaagtct cagggtctac tttatcctca agtcgtgagg gcattctagt ggagtctggg
     3241 gcagagtcaa gagcctgccc gctttcgccg ccgccgcgca gctcggcgcc cagttcccgg
     3301 ggggtcatgc ggctgacgcc cgcccgcgag gccgtgcgct gcacctcctg gtcggccgag
     3361 acgacgacca cctgcacgtc ggcgggctgg ccggcgatac ggcgagcgat gagggtgtcg
     3421 gcggtgaagc gcccgcccgc gaacacgacc tccaccgccg ctcccctgat gggctcgctg
     3481 ctggtgctgc gcgggccggt gccgtcgaag accagcacgg cccgcgtgcc gcgcagcgcg
     3541 acgaagctcg cgaggctgtc ggccagccag tcgcggcgcg cgaagatctc ctccttctcc
     3601 gcgccgcgga acagcgcgtg cagcacgttg tagccgtcga cgatgtagag cgtgccgctc
     3661 accggccgcc gcccgccggg cccttcccgg cggcctcccg ctgccgccgc gcctcgtaga
     3721 gcaggagcgc ggcggccacg ctcacgttga ggctctccac gggcggctcc atcgggatgg
     3781 agagcagcgc gtcgcaggtg cgcgccacga gcggccggag gccgcggccc tccgccccgg
     3841 cgacgagcac gatgcgcccg gtgaggtcgg tgtcgaggta ggacgccgcc gcctcgccgg
     3901 cggcgccgta gacccagaag ccggcgtcgc gcgcccggtg caggaacgcg accacgttgg
     3961 tcacctgggc gaccgccagg agctcgagcg tgccggcgga cgccttgacg gccgcgggcg
     4021 tgacgtgcgc cgagcggtgc ctgggcacca cgagccctcc cgccccggcg gcgagcgcgg
     4081 tccgcgccac cgcgccgagg ttgtgcgggt cggtcacctc gtccaggacc acgagcacgt
     4141 ccgccttcag caggcggtcg gggtccgcgt agggatagcc gtcgacctgc agcgccaggc
     4201 cctggtgctc ccgcgtgccg agcagttcgt cgagctggtg cgcgcttacg gtgtccaccg
     4261 gcaggtcctc gggaacgagg ccggcgacgg cgtcgagggc cggcggggtc gcggcgagct
     4321 tgtgggctcg ccgccgggcg ccggccccga gcgcggcctt gaccacgttg cggccgtaca
     4381 gccactccac ggcggtcacc cctgcccggc cgggccgcgc gcggccgcga gccgcctgta
     4441 gacttgcgac atgcccatcg acttctctcc cggctcgttc gcggcgaccg cgatcgccgt
     4501 gctgttctgg tggaccgtgc tctcgccggc cgccctgctc gcgatcgccg cgctgtgcgg
     4561 gcgccgcccg agcctgcgcg gcgtcctcac tctcgtggtg ctcgtgccgc ccgtggtgac
     4621 gctgctgccc gtggcggtca tgcacggcgg cctcgcggcc gccgccgcgg cggcgggctc
     4681 cctcgtcctc ggcgccgcca tcgtcttgac gatcgtcggc ctctcccggc gcagcgatcc
     4741 ggcgcgcgcc gagcggtacg cctccgccgc cctgctcgcc ggagtcgccg gcggcatgat
     4801 cggcgcgggg gcgctcctgg gggcgcggct cttcggctga cgcgcccggc gcatgccgtt
     4861 cacccgtcct cctcgccccc gctcctacct cttcacgacc tgcgtgccct gcggcgtgtc
     4921 gcgcacctcg aagcccgcgg cctggatctc gtcgcgcagc cggtcggcgt cggcccactg
     4981 cttgccggcg cggtagtggt cgcggaggac gcacgcgtag gaggcgtcgt cacagccgag
     5041 ccggtccgcg tagaccagcg ccacgtcgtc ccagcggccc tcctccgcca gccgggcggc
     5101 cggcagaagg gcgacgccgc cggtggcgca ggcggcggcg tcgtcgccgg cgacgctgcc
     5161 gacggcagtg aggccggcgc cggcccccgc cacggggatc agcagcacgg tgagcgacgc
     5221 gcgcagcacg tcccccaccg cggtcagcgc ccccgtgtcg agcggcgcct ccccgcggtc
     5281 gaccagcgtc aggtagcggt gcatctcgcc cacgagcccg aacagctcac cgatggcccc
     5341 ggcggtgttg aggtcgtcgt ccatccgctc gccgaacgcc acccgcgcgg cctcggcgcg
     5401 cagtgtgagg tccggccact cgccgcgcgc cgccgccctg ctggcgttga cgacgcggaa
     5461 gtcgatgtcg gcgagggcgg aggcgaagcg gccgtaggcg gccttcgcct catccagctt
     5521 ctcggcgctg aactcgagtg ggctgcggta gtgcgtggtg aggaagtacg tgagcaccac
     5581 ggccggctcg tagcggtcga gcacctcccg cagcaggaag atgttgccga cgctcttggc
     5641 catcttctcg ccctcgctgc ggatcatgcc gttgtgcatc cagaccttgg cgaacggcag
     5701 gccggccgcc tcgctctggg cgatctcgtt ctcgtggtgc gggaagatga ggtcgcggcc
     5761 gccgccgtgc acgtcgaacc cggcgccgag gtagcgcagc cccatcgccg agcactcgat
     5821 gtgccagccg ggccggccga gaccccaggg gctctcccag gccggctccc ccggcttggc
     5881 cgccttccac acggcgaagt ccagcgggtc ggccttgccc ggctcggcgt cgacgcgcac
     5941 gccctcctcg agctcgtcga tgcgctgctt gctgagcttg ccgtactgct cgaagctgcg
     6001 caccttgaag tagacgctgc cgccggcctc gtacgcgtgg cccttcgaga tgagctggca
     6061 cacgaggtcg acgatctccg ggatcgtctc cgtggccttg ggctcgacgt cgggccgcgg
     6121 gatgccgagc ctgtcggtgt cggcgacgta ggcggcggtg tactcctgcg cgaccgccgc
     6181 cgagtcgcgt ccctcggcgt tggccttggt gatgatcttg tcgtccacgt cggtgatgtt
     6241 ggtcaccagg gtcacctcca gccctcggta ggcgagccag gagcgcagca cggcgaaggt
     6301 gacgtagggg cgcgcgttgc cgacatgcac caggttgtag accgtgggac cgcagcagta
     6361 gatgccggcc ttgccggggt cgcgaggctc gaacggctcc ttctgctggg tgagggagtt
     6421 gaagaggcgc atcacggtgc ttctcctggt gtcggcgtgg actcaccggc aacgttcccg
     6481 gcgaggatga ggtcgagtcg cgcgagggcc gtcccacggg agagcgcggc gagcacgaac
     6541 ggcaactcgg gcccgtgctc ggccgaggtg agcgcgatgc gcagcggcat gagcaggtct
     6601 cgggcgccga tggagcgctg cttgccccat gcccggtagc cggcgaggag gtcgcgggcg
     6661 ccggcgggcg tgagccactc cggcgcttcg gcgcgcagcg cgcggacctg cgcgagctgg
     6721 ggcgccgccg cgagcgcgac ggcgcggagc tcgtccggcg gcggcggggg cgccagcacc
     6781 tcggccgccg cgcccggcgc ctctccgtag cgcaccagcg acggctggaa cgcggccgcg
     6841 agcgcggccg ccgcctgcgg cggcgtcccg gccggcagcc gctccgcgaa gcgacgctcg
     6901 tgctcctccg gcgcgagcgc catgatgcgc tcgtgatcca gccagtcgag cttggcgagg
     6961 tcgaacacgg cgggactcgc cgagaggctg tcgagctcga actccccgat cagccgttcg
     7021 aggtcgagca cctcgtcctc gccgtgcgac cagccgagca gcgccagata gttcacgatc
     7081 gcctcgggga gatagcccag ctcgcggtag tcgcccaccg cggtcgcgcc gtggcgcttg
     7141 ctgagcttgc cgccgtcggg ccccaggacc atggaatggt gggcgaagcg gggcacgggg
     7201 ccgccgagcg cgtcgaacag cagttgctgg cgcgccgtgt tggtgaggtg gtcgtcgccg
     7261 cggatcacgt gggtgatccc catcgcgaga tcgtcgacca cggtggcgaa gttgtagccg
     7321 gccacgccgt ccgagcgcac gatgatgggg tcgctgagcg cgtcggcgcc gatgacgacc
     7381 tgcccgcgga tccggtcgtc gatgacgacg tcccccgcgg ggaccctgaa gcgcacggcc
     7441 gccggctccc cggcgtccag ccggcgccgc acctcggcgg cgggcagccc gaggcagcgg
     7501 cggtcgtagc gcggcgtgcg gccggcggcg agcgcctcgc ggcgcaattc ctcgaggcgt
     7561 tcctccgggc agaagcagcg gtaggcgagt ccggcgtcca gcaggcgcgc cgcggcctcg
     7621 agatgcccct ccaggcgctc gctctgacga tacgggccgg cgggaccccc gaggtccggc
     7681 ccctcgtccc acgccagccc cagccagcgc aggtccgcca tgatgctctc ctcgagcgcg
     7741 gcgtcggagc gcgccgcgtc ggtgtcgtcc acgcgcagca cgaagctgcc gccgttgtgg
     7801 cgggcgaaga ggaagttgta gagcgcggtg cgcgccgaac cgatgtgcag cgtgcccgtc
     7861 gggctgggtg cgaagcggac tctcactgtg gggtcgaggg ttatgatcgc tctccgaacg
     7921 tcgtcgtttc ccctgcaaac aggtcgagtc tatcaccctg ccttggtatg cttgcggcca
     7981 cgacccggcg agccggcccc ggcccgcccg agccacggga ggaccgagtg ccgcacgacc
     8041 agcgagagac cccctacctc gacgccgcgc tgcgatacaa ggccaccggc tacaccccgt
     8101 tccacacgcc ggggcacaag ctgggcaagg gcgcgccggc cggcctgcgc gagttcgtag
     8161 gcgacgcctg tctctcggcc gacctcgcca tggccggcgc cgtggaggac acgcgcgagt
     8221 ccaccggcct catccgcatc gcggagggat acgccgcgga ggcctggggc tcggaccgca
     8281 cctggttcct ggtcaacggc tccagcagcg gcgtccacgc cctgctgctc acgctgtgcg
     8341 gtcccggaga caccgtgatc gtgccccgca acgcgcacaa gtccatgctc gcggggctca
     8401 tcttcacggg cgccaccccg ctctacatgg agccggcgat cgaccagctc tggggcatcc
     8461 ccctgcaggt acgcgccgcc gacgcgcagc gggctctcga acgggcgccc gacgcccggg
     8521 ccctgttcgt cacctcaccg acctacaacg ggttcggcgc cgacctgcag ggcatcgggg
     8581 ccctggcgca cgccgcgggg atccccttcg tgaccgacca ggcctggggc ccgcacctgc
     8641 gcttctgccc cgagctcccc gtggacgcca tgaccgccgg cgcagacgcc gccgtggtca
     8701 gcacgcacaa gctcatcagc ggcatcacgc agtcggcggt gctcatggcg cgcggcgagc
     8761 gggtcaacct cgcgcgcctc gacgccatgg tccacctgac gcagagcacg agcccgcagg
     8821 tgctcatgta cgccagcatc gatgcggcgc gggcgcagat ggcggtgcag ggcgccgagc
     8881 tctggcgcgg cgcgatcgag ctggcgcagt gggcgcgcgc ggagatctcc cggatgcgcg
     8941 gcctgcgctg cctcggcgcc gagatgctcg ggcgcgaggg cgtcagcgag ttcgacccca
     9001 cccgcctcac cgtctccgcc tgcgacctgg ggctcagcgg gtaccagctc gagacgctgc
     9061 tgcgcgacga ctaccgcatc gccgtggagg cggccgatcc cctcaacatc gtgctcaacg
     9121 tcacgttcgg cgacagtcgc gcggacctgg agacgctcgt cgcggcgctg gccgacctgg
     9181 cggcgcgcta cgcgggcgcc gcggccggcg ccgcggccgc ctgctccacg ctgctcgccc
     9241 acacgccgcc gttcacgcgg caggtcctct ccccgcgcga cgccgtcttc gccccggccg
     9301 tcgcgctgcc gctcggcgac tgcgccgggc aggtgagcgc ggagatggtg acgccgtacc
     9361 cgccgggcat cccggtcctg gggcccggcg aggagatctc gccggagatc gtcgcctacc
     9421 tgcgagaggc cggcgacgca ggcctcaagg tgcacggccc cgaggaccgt acgctgcgct
     9481 ccctgcgcgt cgtcgcctga acccgggggc gcggcgcggc cgccgtcact ccggtttctt
     9541 gatgccgagc gacgtggccg tgaacagcgg gtcgaaggtc agacccttct cctcgatctg
     9601 cgcgcggccg ccctcttcgc ggtcgacgac gcagatgagc gtggagacct ccaggcccgc
     9661 ctcgcgcagc ttgtcggcgg ccatgagcgc ggcgccgccg gaggtcacga catcctcgac
     9721 catcacgacc ctctggcccg gctcggccct gccctccatc gccttgtcgg tgccgtagcc
     9781 cttggccccc ttgcgcacga tcacgaacgg cttgccgctg gcgagcgaga cggcggcgac
     9841 gatggccacg gcgcccagct cggggccggc gagcaggtcg aagggctcgt aggccggcag
     9901 cttgcccgcg atgccggcgg cgatgtcgcg cagcaggtcc ggccgcgtgg agaacaggta
     9961 cttgtcgagg tagaacttgc tgcgccggcc ggaccggagg aggaagtccc cctcgagata
    10021 gctggcgtcg agcaggcgct ggccgaggtc ggtggtcacg gggctccctt cgctggtctc
    10081 tcgggtctgc gggcgcgccg cgtgcgccgc gcccgcagag actgtacagg cggtgaacct
    10141 gcggcgcacc cccagtcgac ccgcctgcgc cggtcgatca ggtgccgccg atggccatcc
    10201 cgtcgatcac gacggacggc gtgaagatgc tgccgccggg gacccagcgg gcgtcgtcgc
    10261 cgagcgcgcg gatgctggtg agcatgccga cgatgtcgcc cgcgagggtg acctcgcgga
    10321 ccggcgtcgt gaacgcgccg tcctcgatga gcacgccgct gatgccgacg ctgaactcgc
    10381 ccgagatggg gttggcgccc gagtggatgc ccacggcgtc ggtcacgagc acgcccttct
    10441 gcatgccggc gacgatgtcg tcgagcggcg tcgccggccc ggtgaccagc aggttgctgg
    10501 ggcgcgcgcc gggcaggctc gagtagccgc cgcgcatgcc gttgccggtg ctctcgcgac
    10561 cggccttgcg cgccgtgtag gtgtcgtaga ggaagccctg gagcacgccc cccgtgatca
    10621 gcggcgtgcg cccgcagggc acgccctcgc cgtcgaacgg cgcgctgtcg agcccgtcct
    10681 cgacggtacc gtcgtcgacc agctcgagga gctcgccggc gacgcgctcg ccctcgcgcc
    10741 cggcgaacag cgagcgcccc ttctgcacgg cgtcggccgt gagcgcggag ctgagcacgc
    10801 cgaagaacgc cgcggagacg aaggggtcga ggatgaccgg cgccttcatc gaagggcact
    10861 tgcgcgcgcc gaggagccgg gtcgcgcggc cggcggcctc ggtgccgacg gcggccgcgt
    10921 ccaggtcctc cagggcgcgg ccgacgctgt aggagtagcc ggtctcgacc tgcccttgtt
    10981 cctcggccag gacgtaggcg aacgcgtagc actggccggc gcggaagctg ccgcgcacgc
    11041 cggcggtgct ggcgatgaag acctcgccct cgccgtcgac gtacatggtg tcttcgaccg
    11101 tcttgacgcg gggatcggcg gcgagagcgg tcgcctcgac ctcgagggcg acggcgatgc
    11161 gctgctcgtc cgtcgcagcc gccagccgcg ggtcgtacgg gtgcacgtcg gcgggctcgc
    11221 cgccgggctg cggcaccgcc gcgaactcgt ccgggtcgct cacggcggcg ttgtcgacgg
    11281 cgcgctgcac gacgtcgtcg agcgcgccgt cggagaggtc ggacgtgtac gcgtggccca
    11341 cggctccgcc ggagaacacg cgcacgccca cgccacggcg ctgcgccgcc gtgagctgct
    11401 ccacttcctg gcggtacacc ttgatgcggt gggacgtgga acgttctccg aacacttcca
    11461 cgtctgccgc gccgcgcgcc tgggcgcgct ccagcacagc gtcgaggatc tcgaacatca
    11521 gcccgtccct cccaccgtca tctcagcgat gcgcagcgtg gcctggccgg tcccgaccgg
    11581 cacgccctga ccgtccttgc cgcagatgcc ggtcttgacg tcgaagtcgc cggcgatcag
    11641 gtcgatcttc atgaggatgt cgatgccgtt gccgacgagg gtggccccgc gcagcggcgt
    11701 cgtgaccttg ccgtcttcga tgagatagcc ctcggcgacg ccgaagacga agttgccgct
    11761 ggccggctcg acctggccgc cggccaggct cttggcgtag aagcccctgg gcgtcgccgc
    11821 gatgatctcc tccgcggtgg cgtcgccggg agcgatgtac gtggtcgtca tgcgcgggat
    11881 cggtacgtgg cggaacgact ggcgccgccc gttgcccgtg ggcgcggcgc ccgcctcgcg
    11941 ggcgcgcagg cggtcgtaga ggtagccggt gagccggccc tcctcgatga ccagcgtctt
    12001 ctgcgtgggc acgccctcgt cgtcgtacgc ctgcgagccc cagccgttgg tgatggaccc
    12061 ctcgtcgtag gcgctcacga tggacgccgc caccacctcg ccgagcttgc cctcgtagat
    12121 gctcgcgccc ttggcgatgg cgtcggcctc gagcccgtga ccgcaggcct cgtggaagag
    12181 cgtgccgccg aacccgttgg ccaggacgac cggcatcgcg ccgaccggag ccggcttgct
    12241 gtcgagcatg atgcgcgcct tgtcggccgc ggtctgcgcg accccgcggg cgatctcctc
    12301 gtcgacgagc tcgaagccgg cgctgccgcc gaccgtctcg tggcccgtct ggatgacctc
    12361 gccgcggcgg gccaccacct gggcgctgaa gcgcgtgcgc gtgcggtcgt cgaacaccag
    12421 gtcgccgagc gagttggcga tgagcacctt ctggcgcgtg tcgccgtacc cgacgatcac
    12481 ctggacgatc tcggacgagg ccgcgcgcgc ggcctcgtcg ccggcgcgca cgagcacggc
    12541 cttgcgcgcg atggcgacct cgccgggggg cgtgagcacg gggttcttgc ccgggtcggc
    12601 caggacggcg ccgagatcga cgatctcggg gcctcgggcg cccgcgctga ccgcagcggc
    12661 gacggcgtcg gccgcggccg tgaggccggc ctcgtcgacg gcgtcgctgt aggcgtagta
    12721 ggtgcgctcg cccgagagca ggcggacggc ggcgccggtc tcccggccgc cgctggtctg
    12781 ctcgatgcgt ccgtcctcga gccgcagccc gagggtctcg cggtcttcga cgaacacttc
    12841 ggcgaagtca ccgccccggc gcagcgctct ctccatggtc gcgcgcagca gggcggtgtc
    12901 gaaggatgcg acgtccatca ggcgcctccg gtgagcgaat cgcggacgcc ggcggccagc
    12961 gtctcgagtt cggcggtaga aggatcgaag aggtccagcg tgcggacggc gccgtcccgc
    13021 gcgccgaact gcacgagcag gtctgccgga gcgggggtcc gccggcgcca ccacacgaga
    13081 ccggcgacgg tgacggcccc cagggccgcg agggcgaccg tgcgggccct catcgcgccc
    13141 cctccgcggc ggcgaacgcg tcgccgagac aggtgcgcag gtcgaagagc agcagcccga
    13201 cggcgggccg gggaccggtg acggccaccg cccggtgacc gtgcgcctcg acgaccgcga
    13261 cgacggcgtc gccggcgggc acgacgacgt gctcgaggcc ggcggttccg gcggcggccg
    13321 cgcgccggct cgccgcgctc cagagcgcct cggcggcctc cgcgaagccg gcgccggcgg
    13381 cgtccggcgc gcacgccacc acgctgccgg acgcgtcgat gacggcgacc accgtgatgt
    13441 cggtcgacac gtcgagcaga tcgcggatcg cttcgtcgat ggtcagggtg tcctccgggt
    13501 gcgcatgcgc ccgtacagta cccgcctggg cggttctctc actcctggcc cgaggcgaag
    13561 gagcagagca gctcgcagcg ctcgccgccg cagctgatgc gacagtcgcc cggctcgatc
    13621 ctcaggggac cgagcccggc cgtggacacc tggaggatgc cctcgaggaa cgcgcggtgc
    13681 atggcgcaga cgaggtcagg gtcggcgccc gacagctccc ggaagctgca gttgttgacc
    13741 acgatgaaga gggcgccgtc cagccagtcc acctcgggga gcaggccctg ctcctcggcg
    13801 atggtggcca ccagaccggc gacggcggcc cggtcgcgtg gcggctccgc gtcgccggcg
    13861 agcgcgcgcc ggccctccgc ggcgccggcc tcgcggcaca cgcggagcgc gtcgtcgcgg
    13921 gtgccgccgg cggcgagcgc gtcgagcgcg agccgcgaga gcagctcgta gcggcgcggc
    13981 gggaacccga aagtgacgac cttgtcgctc agccggtaga gcttcgccgg ccggccgccc
    14041 cctgccgccc ggcggaaccc ggtggcgagg aagcccgcct gctcgagctt ggcgaggtgc
    14101 atgcgggcga cgttggggtg aaggccgaac tcggcggaga cgtcggcgac ggtgacgtca
    14161 gtccccggcc gttcggcgat ccagcggtag atgtggtagc gggtgtcgtc ggccagcacg
    14221 gccgacaact cgtgatcgcg gtccacgcac cttcccctca cctcgagtgg tcccggcgat
    14281 actatcctcg gccacttctc ctgtaaacac ggtgcgtgaa gtgcaaaccg gctcagcgtt
    14341 cgatgacgta gctcgacgtg atcggcagcg ccggcttgag cgtcgccgcc acggagcagt
    14401 agcgctcctc ggacagttcg atggcgcgct ccaccttgcg ttcgtccagg tcgcccttga
    14461 ggtggtagac cacgtcgagc gagaggaact tcttgggatg ctcctccgcc cgctccccgc
    14521 tgacctcgac gcggaagccc tccaggggct ggcgcatctt gaccaggatg gaggcgacgt
    14581 ccatggcggt acaggcgccc agggccgtcg ccagcgtctc catgggcgtc accccgttcc
    14641 ccggctcgtc gagcgccgac gccagggcga tcgtcccgtg cggcgtcgtc gcttcgaagt
    14701 gcaggccctc gcgcttccag tccacgctca ccggtgtgct gcgtgccatc cgcccctcct
    14761 cgctcgctgg accctccgcc tccgcgcggc cgctccagtc accacacagg tagaccgtgc
    14821 gctagactcc tctgcggcag caggcccacg acgcggagag aacacctcat gacgattcta
    14881 caggcgatca tcctcggcat cgtccagggc ctcaccgagt tcctgcccat ctccagctcc
    14941 ggccacctgc tgctcgtccc ctggctgttc aactggcact tcctgctcga gaaccccgag
    15001 ctcaacaaga cgttcgacgt cgccctgcac ctgggcacgt tcgtcgccgt cgtgctgtac
    15061 ttctggcgcg agatcgtgcg gctggtcggc gcctgggtgc gcagcctctc caggcgcagc
    15121 ctggccgagc cggaggccaa gctggcgtgg ctgctgatcg tcagcacggt cccggcggcg
    15181 ctcgtgggcg tgctgttcga ggacttcatc accaacaccc tgggcaagcc gtggatgatc
    15241 ggcgtcgcga tgatcgtctt cgccggcttc atgtacctgg tggaccacat cgccaggctc
    15301 gaccgcgacc tcgaggctct cacctggctc gacgccctgc tcatcggcat cgcccagtcg
    15361 ctggccctcc tccccggggt ctcgcgctcc gggatcacca tgatgaccgg cctgctcgtg
    15421 cgcctcgacc gtgagtcggc cgcgcgctac tcctttctca tgagcatccc ggtcatcggc
    15481 ggcgcggccg cgttcaaggg gctggaggtg gcccgggacg gcctgcccgc cggcaccgcg
    15541 acgccgttcg ccgtcggcat ggtcgcggcg gcgctcagcg gcatcgccgc catctggttc
    15601 acgctcgcct atctcaagcg ccacaacttc aacctgttcg tcgtctatcg catcgtcatc
    15661 ggcgtcggcg tgctcgtcct catcgtcgcc ggcgtccgct cgggcatggc cgtctgagga
    15721 gctggtgccc ctgcggcgca ccgccagatg acgggccttc ggcggtcatt cgtatagaat
    15781 cgggagcccg acgggcccca cagaagcgac caggagtcac cggtgcgcga cctctcccgc
    15841 ggcgaccgcg gcaaagaagt cctcgacgtc cagacccgcc tgcgcggaca gggcttcgag
    15901 ctgggcagag agggtgtgga cggcttcttc gggccgcaca ccgaggctgc ggtcctctcg
    15961 ttccagcagg agcgcggcct gctcgccgac gggcgcgtcg gcgcgaacac ctggcgcgag
    16021 ctcgtggagt ccggctacgc gctcggcgac cggctgctgt acctcaggga gccacccttc
    16081 cgcggcgacg acgtcctccg gctccaggtc aagctcaacc tgctggggtt caacgccggg
    16141 ccggaacgcg gcgtccacga cgacgaggtc gagcgcgccg tgctggactt ccagcgcaac
    16201 gccgggctcc ccgtggacgg catcgtcggc gagagcacgc tccgtaagct cgaggccgtg
    16261 cgcaaggccg agtcgggacg cgagggcaag aagatcccgg agcgcgaccg cggctacgtc
    16321 gccgcccgcg gcctgacggg ccagtccgtg accatcgatc ccgggcacgg cggcgccgac
    16381 agcggcggcg tctcggcgcg gggcctggcc gagaaggacg tgacgctcgc cctgggcctt
    16441 cgcctggccg agctgctgcg cgcggagggc tgccgcgtgc agctgacgcg ggagggcgac
    16501 gagcgcgtcc cgctctacgc gcgcgccgac gccgccaacg gcgcggcctc cgccttcttc
    16561 ctctccctgc actgcaacgg cgactcctcg ccggcggccc gcggcgccgc ggtctactac
    16621 ttccagcgca gtcactacta ctcggagcac gggcgccggc tcgcggggtc catcggcgcc
    16681 cgcatgcggg cggccggcgt gccctggatc ggccgcttcg gccgcaacta cgcgctgctg
    16741 cgggaggcgc gcggcatcgc cctgctggtc gagccgctct tcctcaccag ccccgaggac
    16801 gagacgctcg cccggcggcc ggagcacgtc gagaggctcg cgcgcgcgct ggccggcggc
    16861 ctggcggact acctcgcgcg cgtcccgctc gagggcgagg aggcgtcgtg acgcccgacg
    16921 aggccgacgt cctccgcatc cgcctcacgg cgacgcagtc gcagcgcctg cgtgagctcg
    16981 gcgccgaccc cctggtgctc gacgagctct tcgccgacgc ctcggcgcgc gacgccgcct
    17041 tcaaggccct cgagggcgag ctggtgcgcg acggccgccg gcgcctgcgg gagctgcggg
    17101 agggcaggcg cgcgccgcag ctcgtcgagc tcgagcgtga cctgagcgac gcgctcaccg
    17161 gcgcggggtt cgtgcgcgtc gtcaccccgc tcatcatcgc ctccgacgcg ctgcgcaaga
    17221 tgggcatcga gcacggccat ccactgcgcg agcaggtgtt ctggctcgag gacgggcgct
    17281 gcctgcggcc gatgctggcg ccgaacctct acaccctgct gcggcgcctc gaccgccact
    17341 ggagcaagcc gttcggcatc ttcgagatcg gcccgtgctt ccgccgcgac agcaagggcg
    17401 cgagccacct caacgagttc acgatgctca acctcgtgga gctcggccgc ccggagagcg
    17461 agggccgcgc gcgactcgcc gagctcgcct cggccgtcat gcgcgcggcc ggggtgagcc
    17521 actacgagtt cgaggtgacc gagtccgaag tgtacggcga gatggtcgac gtgctcgtga
    17581 acggcgccga ggtgtgctcc gccggcctcg gcccccaccc gctcgacggg aactggggca
    17641 tcgtcgaccc gtgggtcggg ctcgggttcg gcctcgagcg gctcatcatg gcccgcgaag
    17701 gctacccgaa catcgagcgc gccggccgca gcctgagcta cgtcgacggc gtgcgcctca
    17761 acctctgacc tcggccacca cgctccgcac gtcttccggg ccgagaggta cgcgcccggg
    17821 cgcagtcgtg cgccggcgcg accgcagccg ccgggccctc cggcgccgta cgcgcgaggg
    17881 gcgggccgac cggcccgccc ctcggacgtt cagctgctgc gtgtgcgtcc tgcttacttc
    17941 cccagcagcg cgtcgatctt cttgacgccg tcgttggcgt cctcggcgta ggcgtcggcg
    18001 ccgatctggt cggcccagcg ctgcgtgcag ggggcgccgc cgacgacggt cttgaagggc
    18061 aggccctcgt cc
//
LOCUS       DASUMX010000024         9206 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_581334, whole genome shotgun sequence.
ACCESSION   DASUMX010000024 DASUMX010000000
VERSION     DASUMX010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 9206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9206
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581334"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(205..1335)
                     /locus_tag="VFZ86_01155"
     CDS             complement(205..1335)
                     /locus_tag="VFZ86_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5640925.1"
                     /translation="MRDVQETGGRRSFGRGFRQRSPLVLVAAALMTVAVLLGGQALLF
                     DGGLTAVDEGRLLRITHDDYVHVSVRVQELRKDPPATRTVYLFGGSGTMECFISEASL
                     ASAISREAGREVRVVSLAAHQESMAMSLALIDNLPRGPATLALGLAPIRVTGAPARDA
                     EMLSGHTLLVRSPRLEALAPRHLGAKAPATGAIPGLFDYVGSYLKERAAASALWGTPI
                     VYETHYYPWGAKGHLPLGKRRNVLYVLEKDVELYRKYADYNFAMLEEILKLADERGFD
                     VVLYDQPLNASAAGPDWNGVVPAYRKRARELAARYDVPYVHIERGVKLTDDDFADLFH
                     LLAPARLKWQPEMARQLAAALDGTSGPVAAAPSAPHPAAALR"
     gene            complement(1403..2650)
                     /locus_tag="VFZ86_01160"
     CDS             complement(1403..2650)
                     /locus_tag="VFZ86_01160"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HEX5640926.1"
                     /translation="MKIYFTLYLIPVVIIALLVFWLALRKDAHRLIFILLLSVAVLAL
                     LHPVFALVAVGLVLVTHQLVELQRRERMRGARVVLIAIVVALVTLGIGKYGQRGAIAV
                     WGEGDPIVTHLIMPLGISYFVFRMLQYVFDCMRGVIKENSLLRLAAFMMFIPTFPAGP
                     LETYQGFYGKRTPGFDRQEFYYGLRRIALGYFKKVFVVDFLFSIFFGKIITTIGRTDF
                     DPGDVGTWWPLAYVIIVFVRAYFDLSAYTDLAIGFSRLFGFRIMENFHYPFWQKNLSD
                     FWKSWHISLSTWVRNNVYFPVFGLTRKVWVGLYASMLVMGLWHAVDLNWTMWGLYHGT
                     GLVIVARWERWKKKRKKARKKAGLPPAARWERFTRWLGYPATFLYVAIGYAFVSTHSF
                     EQALKILFNLFRLPLAWLAGVVL"
     gene            complement(2647..3717)
                     /locus_tag="VFZ86_01165"
     CDS             complement(2647..3717)
                     /locus_tag="VFZ86_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5640927.1"
                     /translation="MGAFRRGFHNRSPLVLVAAAALLVPALLGFQWLFTSGAWTAVPL
                     DAALRNPEDSFTYISWVVGKARQDDPRVPGLYLLGGSSARESIESGDALAAEIADLGG
                     PRVAAHNLGSINQNFAQSLAVVDSVPDTPAVLLVGVNLGRFTATREQNAAQAEGRELL
                     LESGSVREFVSSAWGLKEQSYTILPGIWAYLTDWAREHGGRLLEGDPPSTEYELHRYS
                     AKTRRSDDQKKRLVTKWNATRRPVFEKNLGANLEMLEALLAQARERGVRVVLLELPLN
                     EEIVGDAFAASQRRYREPVAALARDYDADYVDLNQTLDIPSRDFQDLSHLLAPGRAVW
                     QHALARELTRLISTEDSGGSSG"
     gene            complement(3717..4787)
                     /locus_tag="VFZ86_01170"
     CDS             complement(3717..4787)
                     /locus_tag="VFZ86_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5640928.1"
                     /translation="MNSFRRGLRNRNPLVLVAALALLVPALLGIQSWFTSGAWSAVPV
                     QRWVRNATDDYMVVSWTVGRLKRHPPEVPLAVLLGGSSGREAIISGPSLAREVEAEGG
                     PEIDAYNLSSRMQRYAQSWAIVDNLPDTPTTVLVGVNLGRFTTSPRSNYNQVIGRELL
                     LKSDSLRRWVNDYSGEHAHSYTILPGIFSALASYLQQRENDLLYKGRLHPRPYRPHPF
                     DKRKALPDAEKQELVQKWLARYYPAFKKVHRFDAAMLDGLIAEGERRGLDMVIVELPW
                     NQEVVGESFDAALAAYRAPTTAIAAKHGVPYLDFNEELAIPSAYFLDLSHLRPKGRAI
                     WQAELARRLASMYRDGTLRTEL"
     gene            complement(4804..6321)
                     /locus_tag="VFZ86_01175"
     CDS             complement(4804..6321)
                     /locus_tag="VFZ86_01175"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HEX5640929.1"
                     /translation="MTDAVARLRRWAAERADATLLRTRDRTYTYADMEARATRVAAAL
                     RAAAGRRGARVALVLEEYDQFFVCMLGAWLAGQVVVPLNTSLPQHDIDWLLGKARPDV
                     IVLPDDTACPTAEDGPVRLVVGADAGGLVDGVRAAGGVAAPAVPAEGLEAVREDEQAM
                     IMFTSGTTGLPKGVCQTLRAVSGNAGHVALVLGLEADDRIFINTPPYFTSGICHFLTL
                     LAHGGGVAGQLGFYFGAGLLDAMQAEGCTGFGGAPAHLVRVVEPLEGPREHAFRFWVS
                     SGDHLPLPVIEKFRRVLPGVRLFNMYGLTEVSGRLCVLPPAELDVKVGSVGRPIGDMR
                     VVARRGDLTEAAPGEQGELYVTGSLLMQGYLDEAEITAQTLTAHGLKTGDFGKVDESG
                     AVWIAGRKDDIIKRGGEKVSIIHVQEALQGLGRFADVAVVAAPDEILGHVPVAFVVPN
                     EPVGFKASKLLRELRGVLPSSSLPSRVIAVAQIPRTGSGKAIRAELRRMLAEESG"
     gene            complement(6358..6555)
                     /locus_tag="VFZ86_01180"
     CDS             complement(6358..6555)
                     /locus_tag="VFZ86_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5640930.1"
                     /translation="MPEQGAEHLPAEDAESPEPARSRGHWFYYGLGLSLQTFLWFLLF
                     LAITVAVLVGGKLTEFRYVGF"
     gene            complement(6548..6796)
                     /locus_tag="VFZ86_01185"
     CDS             complement(6548..6796)
                     /locus_tag="VFZ86_01185"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX5640931.1"
                     /translation="MSDADTILEFIRDEADLEQDEVNADTSLFRDQLLDSMNLTALIA
                     FLEEEFAIKVRPMDIVYENLDTVNHMLAYVARKKAGDA"
     gene            complement(6828..7511)
                     /locus_tag="VFZ86_01190"
     CDS             complement(6828..7511)
                     /locus_tag="VFZ86_01190"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HEX5640932.1"
                     /translation="MTVRLLGLNCSPRDNSNSSIMLETAFEKLDATYPGECEHSVISL
                     RDLHVEPCKACNVCGKKKDGAFIPCVREKEDDVQGVLDAMVAADGIVVATPVYFGLPS
                     DLFSKFIMRTRPLRHQDFKLANKPVGVLATAGRRSGGAETAIIATWLPFVRNGCLVVG
                     NGDGSCQFGAYGWAGGRGHILSDEWGLEQAFQTTQRVFTLAGLLKAGAEATGYVNYMK
                     FSYSQGTRT"
     gene            complement(7644..7731)
                     /locus_tag="VFZ86_01195"
     tRNA            complement(7644..7731)
                     /locus_tag="VFZ86_01195"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7695..7697),aa:Leu,seq:caa)
     gene            complement(7813..8622)
                     /locus_tag="VFZ86_01200"
     CDS             complement(7813..8622)
                     /locus_tag="VFZ86_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5640933.1"
                     /translation="MSRSKSWIAVVAITVAVAALAAVALPQKAAATSLKSRLISAKRV
                     LRAHNHRLTAAEAALAAAAAGTSLALVDPVVTPAPTALPTPDASPTPDITPTPDITPT
                     PEATPTPEATPTADMTPAPVATPVTVAATPTVEELRARVAKARRAVRIWKLRVHRLSK
                     QYRFRLRMAEWERRGQWMPIIRVAAARYHVSAGGMYRMMMRESGGRRFAGSSSAFKGL
                     FQYYTGTWAAGWNPYRHDSIYDGSSQIFATAYAISRGMGRQMWTTTFASQY"
BASE COUNT         1386 a         3153 c         3320 g         1347 t
ORIGIN      
        1 agtcgcgcac ggtccaggag cgccagccga cgacgccggc cacggccaag ccggcgacca
       61 cgacgccacc gccgacgagg agctggcgac gggtcatccc ggcctccggg ccggagacag
      121 ggtcctgcgg cgcgcctccg gccggcgcgc ctccggcctg ccggccggca cgaggatgcg
      181 gcggcgctcc ggacgcgccc ggcgtcatcg cagcgctgcc gccggatgcg gcgcggacgg
      241 cgccgccgcg accggcccgg acgtcccgtc cagcgcggcg gccagctgcc gcgccatctc
      301 cggctgccac ttgagccgcg cgggcgcgag caggtggaag aggtcggcga agtcgtcgtc
      361 ggtgagcttc acgccgcgct cgatgtgcac gtagggcacg tcgtagcgcg cggcgagctc
      421 gcgggcgcgc ttgcggtagg ccggcaccac gccgttccag tcggggccgg ccgccgacgc
      481 gttgagcggc tggtcgtaga gcaccacgtc gaagccgcgc tcgtccgcca gcttgaggat
      541 ctcctccagc atggcgaagt tgtagtccgc gtacttgcgg tacagctcaa cgtccttctc
      601 gagcacgtag agcacgttgc ggcgtttgcc cagcggcagg tgccccttgg cgccccaggg
      661 gtagtagtgc gtctcgtaga cgatcggcgt gccccagagc gcgctcgcgg cggcccgctc
      721 cttgaggtac gagcccacgt agtcgaagag gccggggatc gcgcccgtgg ccggcgcctt
      781 cgcgcccagg tggcgcggcg ccagggcctc gaggcgcggg ctgcgcacga gcagcgtgtg
      841 gccgctcagc atctcggcgt cgcgcgcggg cgcgccggtg acacggatgg gcgcgaggcc
      901 gagcgcgagc gtagccgggc cgcgcggcag gttgtcgatc agcgccaggg acatcgccat
      961 gctctcctgg tgcgcggcga ggctcacgac ccggacctcc cggcccgcct cgcggctgat
     1021 ggcggaggcc agtgaggcct cgctgatgaa gcactccatg gtgcccgacc cgccgaagag
     1081 atagaccgtg cgcgtcgccg gcgggtcctt gcgcagttcc tgcacgcgca ccgacacgtg
     1141 cacgtagtcg tcgtgcgtga tgcgcaggag gcggccctcg tcgacggcgg tgagaccgcc
     1201 gtcgaacagc agcgcctggc cgccgagcag gacggcgacc gtcatcaggg cggccgcgac
     1261 cagcaccagc gggctccgct ggcggaaccc gcggccgaac gaccgccggc cgccggtctc
     1321 ctgtacgtca cgcacgttca ctctccaccc tcaccgatat gtccatggtc tcacagtaca
     1381 acgctcgcgg ttcgcgccgg gttcacagca cgacgccggc gagccacgcc agcggcaggc
     1441 ggaagaggtt gaacaggatc ttgagcgcct gttcgaagct gtgcgtggag acgaacgcgt
     1501 agccgatggc cacgtagagg aaggtcgccg gatagcccag ccaccgggtg aacctctccc
     1561 accgggccgc cggcggcagg cccgccttct tgcgcgcctt cttgcgcttc ttcttccagc
     1621 gctcccagcg ggcgacgatc acgaggccgg tgccgtgata cagaccccac atggtccagt
     1681 tgaggtccac ggcgtgccag aggcccatga cgagcatgct ggcgtacaga ccgacccaca
     1741 ccttgcgcgt gaggccgaac accgggaagt acacgttgtt gcgcacccag gtgctgaggg
     1801 agatgtgcca gctcttccag aagtcgctca ggttcttctg ccagaagggg tagtggaagt
     1861 tctccatgat ccggaatccg aagaggcgcg agaagccgat cgcgaggtcc gtgtaggcgg
     1921 agaggtcgaa gtaggcgcgc acgaacacga tgatcacgta ggcgagcggc caccacgtgc
     1981 ccacgtcccc ggggtcgaag tccgtgcgcc cgatggtggt gatgatcttg ccgaagaaga
     2041 tcgagaagag gaagtcgacg acgaagacct tcttgaagta gcccagcgcg atgcgcctga
     2101 ggccgtagta gaactcctgg cggtcgaacc cgggggtgcg cttcccgtag aagccctgat
     2161 acgtctccag cgggccggcg gggaacgtcg ggatgaacat catgaacgcg gccagccgca
     2221 gcaggctgtt ctccttgatc acgccgcgca tgcagtcgaa cacgtactgg agcatgcgga
     2281 acacgaagta gctgatgccg agcggcatga tgaggtgcgt gacgatgggg tcgccctcgc
     2341 cccagacggc gatggcgccg cgctggccgt acttgccgat gccgagcgtg accagcgcga
     2401 cgacgatggc gatgagcacg acgcgcgcgc cgcgcatcct ctcgcgccgc tggagctcga
     2461 cgagctggtg cgtcacgagc accaggccca cggccacgag ggcgaagacc gggtgcagca
     2521 acgccagcac ggcgacgctg agcagcagga tgaagatcag gcggtgggcg tccttgcgca
     2581 gcgcgagcca gaacacgagc agcgcgatga tgaccacggg gatcaggtac agcgtgaagt
     2641 agatcttcac ccgctactcc ctccagagtc ctcggtgctg atgaggcgcg tcagctccct
     2701 tgccagcgca tgttgccaga cggcacgccc gggcgcgagg agatgcgaga gatcctggaa
     2761 gtcccggctg gggatgtcga gcgtctggtt gaggtcgacg tagtccgcgt cgtagtcgcg
     2821 ggcgagcgcc gcgacgggct ccctgtaccg ccgctgcgac gctgcgaagg cgtcgccgac
     2881 gatctcctcg ttgagcggca gctcgaggag cacgacgcgc acgccgcgct cgcgtgcctg
     2941 cgccagcagc gcctcgagca tctccaggtt ggcgccgagg ttcttctcga agacgggtct
     3001 gcgggtcgcg ttccacttcg tcacgagccg cttcttctgg tcgtccgagc gacgcgtctt
     3061 cgccgagtac cggtgcagct cgtactcggt gctcggcggg tcgccctcga gcagcctgcc
     3121 gccgtgctcg cgcgcccagt cggtcaggta ggcccagatg ccggggagga tcgtgtacga
     3181 ctgctccttg aggccccacg cggaagagac gaactcccgg accgagccgc tctcgagcag
     3241 gagctcccgt ccctcggcct gcgccgcgtt ctgctccctg gtcgcggtga agcggcccag
     3301 attgacgccg acgagcagca cggccggcgt gtccgggaca ctgtccacga cggccaggct
     3361 ctgggcgaag ttctggttga tcgagccgag gttgtgggcg gcgacgcggg gcccgccgag
     3421 atcggcgatc tccgcggcga gcgcgtcgcc gctctcgatc gactcacgcg cgctcgatcc
     3481 gccgagcagg tacaggcccg gcacccgggg gtcgtcctgc cgggccttgc ccaccaccca
     3541 cgagatgtac gtgaacgagt cctcggggtt gcgcagtgcg gcgtcgagcg ggaccgccgt
     3601 ccaggccccg ctcgtgaaca gccactggaa gccgagcagc gccggcacga gcaaggccgc
     3661 ggccgcgacc agcacgagcg ggctgcggtt gtggaacccg cgccggaacg cgcccatcag
     3721 agctccgtcc gcagggtgcc gtcgcggtac atcgacgcca ggcggcgggc gagctcggcc
     3781 tgccagatcg cccgcccctt ggggcgcagg tgcgagaggt cgaggaagta cgcgctgggg
     3841 atcgccagct cctcgttgaa gtccaggtac ggcacgccgt gcttcgcggc gatggccgtg
     3901 gtgggcgccc ggtaggccgc cagcgcggcg tcgaacgact cgccgacgac ctcctggttc
     3961 cacggcagct ccacgatcac catgtcgagc ccgcgtcgct caccctcggc gatgaggccg
     4021 tcgagcatgg ccgcgtcgaa gcgatggacc ttcttgaacg ccgggtagta ccgcgcgagc
     4081 cacttctgca ccagctcctg cttctccgcg tccggtaagg ccttgcgctt gtcgaagggg
     4141 tgcggcctgt acggcctggg gtgcaggcgg cccttgtaca ggaggtcgtt ctcgcgctgc
     4201 tgcaggtacg acgcgagggc ggagaagatg cccggcagga tggtgtagga gtgcgcgtgc
     4261 tcgcccgagt agtcgttgac ccagcggcgc aacgagtcgc tcttgagcag cagctcgcgg
     4321 ccgatcacct ggttgtagtt gctcctgggg ctggtcgtga agcggccgag gttgacgccg
     4381 acgagcaccg tcgtcggcgt gtcgggcagg ttgtcgacga tcgcccagct ctgcgcgtag
     4441 cgctgcatgc gggagctcag gttgtaggcg tcgatctccg gcccgccttc cgcctccacc
     4501 tcgcgggcca gcgaggggcc gctgatgatg gcctcgcggc cgctcgaccc gccgaggagg
     4561 acggcgagcg ggacctccgg cgggtgccgc ttgaggcgac cgacggtcca ggagaccacc
     4621 atgtagtcgt cggtggcgtt gcgcacccag cgctgcacgg gcacggcgct ccaggcgccg
     4681 ctcgtgaacc agctctgaat gccgagaagc gccggcacga ggagcgccag cgccgcgacg
     4741 agcaccagtg ggttcctgtt cctcaggccg cgcctgaagc tgttcacgcc ggcccctcga
     4801 ggttcacccc gactcctcgg cgagcatcct gcgcagctcg gcgcggatgg cctttcccga
     4861 tccggtgcgc gggatctgcg cgacggcgat cacgcggctc ggcagggacg aggacggcag
     4921 cacgccgcgc agctcgcgca ggagcttcga cgccttgaag cccacgggct cgttggggac
     4981 cacgaaggcg accggcacgt ggccgaggat ctcgtcgggc gcggccacga cggccacgtc
     5041 ggcgaagcgc ccgagcccct gcagcgcctc ctgcacgtgg atgatgctga ccttctcgcc
     5101 gccgcgcttg atgatgtcgt ccttgcgccc ggcgatccac acggcgccgc tctcgtcgac
     5161 cttgccgaag tcgccggtct tgaggccgtg cgccgtgagc gtctgcgccg tgatctccgc
     5221 ttcgtcgagg tagccctgca tgagcagcga cccggtgacg tacagctccc cctgctcgcc
     5281 gggcgcggcc tccgtgaggt cgccgcgccg cgccaccacg cgcatgtcgc cgatgggccg
     5341 gccgacggaa ccgaccttga cgtcgagctc ggccggcggc agcacgcaca gccggccgct
     5401 gacctcggtg agcccataca tgttgaacag gcgcacgccc ggcagcacgc ggcggaactt
     5461 ctcgatcacc ggcagcggca ggtggtcgcc gctgctgacc cagaagcgga aggcgtgctc
     5521 gcgcggaccc tcgagcggct ccacgacgcg caccaggtgc gcgggggcgc cgccgaaccc
     5581 cgtgcagccc tccgcctgca tcgcgtcgag cagcccggcg ccgaagtaga agccgagctg
     5641 gccggccacg ccgccgccgt gcgccagcag cgtcaggaag tggcagatgc cgctggtgaa
     5701 gtacggcggc gtgttgatga agatgcggtc gtccgcctcg agcccgagca cgagcgccac
     5761 gtggccggcg ttgccggaga cggcgcgcag cgtctggcag acgcccttgg gcaggccggt
     5821 cgtcccggac gtgaacatga tcatcgcctg ctcgtcctcg cgcaccgcct cgagtccttc
     5881 cgccgggacg gcgggagcgg ctacgccgcc cgccgcccgc acgccgtcga cgaggccgcc
     5941 ggcgtcggcg ccgaccacga gacgcaccgg gccgtcctcc gcggtcgggc aggccgtgtc
     6001 gtcggggagc acgatcacgt ccggccgcgc cttgccgagc agccagtcga tgtcgtgctg
     6061 cgggaggctc gtgttgagcg gcacgaccac ctgtccggcc agccatgcgc cgagcatgca
     6121 cacgaagaac tggtcgtact cctcgagcac cagcgcgacg cgggcgccgc ggcggccggc
     6181 ggctgcgcgc agcgccgccg cgacgcgcgt cgcgcgcgcc tccatgtccg cgtacgtgta
     6241 cgtgcggtcg cgcgtccgca gcaacgtggc gtccgcgcgc tcggccgccc agcggcgcaa
     6301 acgcgcgacg gcgtcggtca tcaggggttc aggcgacatg agcggccggg agaggggtca
     6361 gaagccgacg tagcggaact cggtgagctt gccgcccacg agcacggcga cggtgatcgc
     6421 caggaacagc aggaaccaga ggaacgtctg caggctcagc ccgaggccgt agtagaacca
     6481 gtggccgcgg gacctggccg gctccgggct ctccgcgtcc tccgccggca ggtgctccgc
     6541 gccctgctca ggcatcgccc gccttcttgc gcgcgacgta ggcaagcatg tggttcacgg
     6601 tgtcgaggtt ctcgtagacg atgtccatgg gcctcacctt gatggcgaac tcctcctcga
     6661 ggaacgcgat gagcgcggtg aggttcatgg agtcgagcaa ctggtcgcgg aagagcgacg
     6721 tgtcggcgtt gacctcgtcc tgctcgaggt cggcctcgtc gcggatgaac tcgaggatcg
     6781 tgtcggcgtc ggacatggcg gttccttccg gggagaggcg aggggcctca ggtgcgggtg
     6841 ccctgcgagt acgagaactt catgtagttc acatagcctg tggcctctgc gccggccttg
     6901 agcaggccgg cgagcgtgaa cacccgctgc gtggtctgga aggcctgctc cagcccccac
     6961 tcgtcgctga ggatgtggcc acggccgccc gcccatccat aggcgccgaa ctggcaactg
     7021 ccgtcaccgt tgcccacgac caggcagccg ttgcgcacga agggcagcca ggtggcgatg
     7081 atggcggtct cggcgccgcc ggagcggcgg ccggcggtgg ccagcacgcc gacgggcttg
     7141 ttggccagct tgaagtcctg gtggcgcagc gggcgcgtgc gcatgatgaa cttgctgaag
     7201 aggtcggacg gcaggccgaa gtagacgggc gtggccacca cgatgccgtc ggcggcgacc
     7261 atggcgtcga ggacgccctg gacgtcgtcc tccttctcgc ggacgcaggg gatgaaggcg
     7321 ccgtccttct tcttgccgca cacgttgcag gccttgcacg gctcgacgtg caggtcgcgc
     7381 aggctgatca cgctgtgctc gcactcgccc ggataggtgg cgtcgagctt ctcgaacgcc
     7441 gtctcgagca tgatgctgct gttgctgttg tcgcgggggc tgcagttgag ccccaagagc
     7501 cgcaccgtca tgcgtcgcgc tcctctccgt gcacgccgcc cgacgagaaa cgcagggcgg
     7561 agggaatcgg tcatggcccg cagcctcagt acgggaatga aatcctacca gacggctgtc
     7621 tcacgcgccc gcgaggcggg ggttggtgcc ggaggtggga ctcgaaccca cacgcccttt
     7681 cggggcaccc gattttgagt cgagcgcgtc taccagttcc gccacaccgg cacgggaggc
     7741 ttgcagacac ccattctgac gcatccggcg tgccgcgcgc acctttggcg gcggcgcccc
     7801 ggcttcacgc cgtcagtact gcgaggcgaa ggtggtcgtc cacatctgcc ggcccatgcc
     7861 cctgctgatg gcgtaggcgg tggcgaatat ctgcgagctg ccgtcgtaga tgctgtcgtg
     7921 gcggtaaggg ttccagccgg ccgcccaggt gccggtgtag tactggaaca ggcccttgaa
     7981 cgcgctgctc gaaccggcga agcgccggcc gccggactcg cgcatcatca tgcggtacat
     8041 gccgccggca ctgacgtgat acctcgcagc ggcgaccctg atgatcggca tccactgccc
     8101 gcggcgctcc cactcggcca tcctcaaccg gaagcgatac tgcttggaga gacggtgcac
     8161 gcgcagcttc cagatgcgca cggcgcggcg ggccttcgcg acccgcgccc tgagctcctc
     8221 gaccgtgggg gtcgcggcaa cggtgacggg tgtggcgacc ggtgcggggg tcatgtccgc
     8281 cgtcggcgtg gcctccggcg tcggcgtggc ctccggcgtc ggcgtgatgt ccggcgtcgg
     8341 cgtgatgtca ggcgtcggcg aggcgtcagg cgtcggcagc gccgtcggcg ccggggtgac
     8401 gacggggtcc acgagggcga ggctggtgcc tgctgcggcg gcggccaggg cggcctcggc
     8461 ggccgtgagc cgatggttgt gcgcgcgcag gacgcgcttc gcggagatca gcctgctctt
     8521 gaggctggtg gccgcggcct tctgagggag ggctacggcg gccagggcgg cgacggcaac
     8581 ggtgatcgcg acgacggcga tccagctctt gctgcgactc acaacgtcct ttcgacgtgg
     8641 tctcccccgg cgcagcggct tcccggcctg tgcggagggg cgttcggcga tgggacgcgc
     8701 aaggcccgca tgcgaacgcc gcagaagggg gtgtgagtga agaaggggcg aacgggatgt
     8761 gggcggcgcg tcggacgcct cgttgttcgt tcgggaccga actcaaccaa atgcaacggc
     8821 gttctgcaac ccatagcgcc tgccagagac ctctcccaga aggcgcgccg gcgcgcccgg
     8881 cgagccccct cacgggcgcg atgcgccacc ctgcaggctc ggggatcggg cgacactcat
     8941 gttctggtcg aaggcctgct catgcagtgc gcgaggcgcg tccacccagt gcatgagacg
     9001 acgttcagcg gctgcttgag ggtcgcctca agcgccggtc gagggagcgc gaagactggc
     9061 ctcgaccacc cagccggcga cgtgcgtacg cgcgctcacg acgacgaacc ccgcctcgcg
     9121 tgcggagcgt agcgtgtccc ggtcgagacg gcagccgccg gcgaccgacg cccaggccgg
     9181 ggccgccctg ctctggaggg cccgcc
//
LOCUS       DASUMX010000025         2113 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1588437, whole genome shotgun sequence.
ACCESSION   DASUMX010000025 DASUMX010000000
VERSION     DASUMX010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1588437"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..458)
                     /locus_tag="VFZ86_01205"
     CDS             complement(15..458)
                     /locus_tag="VFZ86_01205"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX5640934.1"
                     /translation="MRRAKGVVVDASVALTAVLLEPLHSQSLAVLDQSAAPGSRGLCP
                     DVFDTECAAGIVKAVRQGRLDADLIERTWSSIAGLPLDRHPTLEIGADALQVALDHGI
                     SAYDAMYVALSLSTGLPLVTADNRLVRMLSGTGCEVIPLDEITVT"
     gene            complement(455..706)
                     /locus_tag="VFZ86_01210"
     CDS             complement(455..706)
                     /locus_tag="VFZ86_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5640935.1"
                     /translation="MATLHVRNVPDELYAKLKALAEEDHRSLSSEVVWLLGEAAVAGS
                     RRRRMRTALDALHQIALENGPQPAGTAARLIREVRDEES"
     gene            complement(915..1472)
                     /locus_tag="VFZ86_01215"
     CDS             complement(915..1472)
                     /locus_tag="VFZ86_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015902285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HEX5640936.1"
                     /translation="MAAKKITTPLADEVVKDLRAGDAVEITGVVYQARDAAHKRLVAL
                     IEAGEELPFDLAGAVVYYMGPSPAKPGNVIGSAGPTTSGRMDAYAPLLMRHGLKGMIG
                     KGLRTAEVKQAMQDEGAVYFAATGGAGALLAKRIVGNEVIAYPELGAEAIARLEVVDF
                     PAIVVMDCHGGDLYAEGRAEYAREA"
     gene            complement(1485..>2113)
                     /locus_tag="VFZ86_01220"
     CDS             complement(1485..>2113)
                     /locus_tag="VFZ86_01220"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HEX5640937.1"
                     /translation="IFAEVGQDVHLAGGDFEEAVHEGVRQGYGDGYLRKSVAEEPGHA
                     RRNTRDNTPAIIHTSIVPGDWVTLTMMAKGGGAENMSSLNMLKPARGWTGIVDAVVET
                     VSRAGSNPCPPVIIGVGMGGTIEKVTLLAKKALLRDVGSTHPDPRIAAMEDELLEKIN
                     ALGIGPQGLGGRTTALDVFIEEMPCHIASMPMAVNVQCHAQRHKTVTL"
BASE COUNT          304 a          744 c          711 g          354 t
ORIGIN      
        1 ttccggaggc ggaatcacgt cacggtgatc tcatccagag gaatgacctc gcagcccgtg
       61 ccggagagca tccgcacaag ccggttgtcg gccgtcacca gaggcagacc cgtcgacaga
      121 gacagcgcta cgtacatggc atcgtaggcc gagatgccgt gatcgagcgc gacttgcagc
      181 gcgtcggcgc cgatctccag agtgggatgt cgatccaagg gcaagcctgc aatgctcgac
      241 caagtgcgct cgatgaggtc cgcgtcaagg cgtccctggc ggacggcctt gacgatccct
      301 gccgcgcact ccgtgtcgaa cacgtccggg cagagacccc ttgatccggg cgcagccgac
      361 tggtccagga ccgccagaga ctgtgagtgc agcggctcaa gcaggacggc cgtcagcgcc
      421 acgctggcat cgacgacgac gcctttggca cgcctcacga ctcctcgtcc ctgacctctc
      481 gaatcagccg cgcagccgtc cctgcgggtt gcggcccgtt ctcgagcgcg atctggtgga
      541 gggcatccag cgccgtcctc atacgccgtc gtcgagagcc ggctaccgct gcctctccga
      601 ggagccagac gacctcggac gagagcgacc tgtgatcttc ctcagccagc gccttcagct
      661 tcgcgtacag ctcgtccggc acgttgcgca cgtggagtgt ggccattccg gacctccctt
      721 ccctcccaac gtacccggcg tggttgcatt ctgcaacacg ctggggtgag ccgggaaatc
      781 gggtcgggcc ggcgatatga ggttctgagg aggagcgagt catgggatga gtctgactgc
      841 gccccggcgg ctgagccggc gcgcgagcac cctcgcgccc gaggccccga gctccgcggc
      901 gcgcgcgcgt cgcctcaggc ctcgcgcgcg tactccgcgc gaccctccgc gtacaggtcg
      961 ccgccgtggc agtccatgac gacgatcgcc gggaaatcca cgacctcgag ccgggcgatc
     1021 gcctcggcgc ccagctcggg gtaggcgatc acctcgttgc cgacgatgcg cttggcgagc
     1081 agggcgccgg cgccgccggt cgcggcgaag tagacggcgc cctcgtcctg catggcctgc
     1141 ttgacttcgg ccgtgcgcag ccccttgccg atcatgccct tgaggccgtg ccgcatgagc
     1201 agcggcgcgt aggcgtccat gcggccgctg gtggtaggcc cggccgagcc gatcacgttg
     1261 ccgggcttgg ccgggctcgg ccccatgtag tagacgacgg cgccggcgag gtcaaagggg
     1321 agctcctcgc cggcctcgat gagcgcgacg agccgcttgt gcgccgcgtc gcgcgcctgg
     1381 tagaccacgc cggtgatctc cacggcgtca ccggcgcgca gatccttcac gacctcgtcg
     1441 gcgaggggag tcgtgatctt cttcgcagcc atggtcccct cccttcagag cgtgacggtc
     1501 ttgtgccgct gggcgtgaca ctggacgttg acggccatcg gcatgctggc gatgtggcac
     1561 ggcatctcct cgatgaacac atcgagcgcg gtggtgcggc cgcctagtcc ctgcgggccg
     1621 atgccgagcg cgttgatctt ctcgagcagc tcgtcctcca tcgccgcgat gcgcgggtcg
     1681 ggatgtgtgc tcccgacgtc acgcagcagc gccttcttgg cgagcagcgt gaccttctcg
     1741 atggtgccgc ccatgccgac gccgatgatc acgggcgggc acgggttgga gccggcgcgg
     1801 ctcaccgtct cgacgacggc gtcgactatg cccgtccagc cccgcgccgg cttgagcatg
     1861 ttcagcgagc tcatgttctc ggcgccgccg cccttggcca tcatcgtcag ggtgacccag
     1921 tcgcccggga cgatgctggt gtggatgatg gccggcgtgt tgtctctggt gttccgccgc
     1981 gcgtgccccg gctcctccgc gacggacttg cgcaggtagc cgtcgccgta cccctgccgc
     2041 acgccctcgt ggacggcctc ctcgaagtcg ccgccggcga ggtgcacgtc ctgccctacc
     2101 tcggcgaaga tca
//
LOCUS       DASUMX010000026         3693 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_7164, whole genome shotgun sequence.
ACCESSION   DASUMX010000026 DASUMX010000000
VERSION     DASUMX010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3693
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7164"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="VFZ86_01225"
     CDS             complement(<1..387)
                     /locus_tag="VFZ86_01225"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5640938.1"
                     /translation="MDAGSETPSELRGALVTLRPVAPADLPALLDILDEPEVARWWRR
                     AEWERVDEVGAVTLTILLPDATVAGCIQYSEETDPDYFSAAVDIFVGTAAQGRGVGPD
                     AMRTLAAWLVDARGHHRLTVDPALENA"
     gene            complement(398..1559)
                     /gene="prfB"
                     /locus_tag="VFZ86_01230"
     CDS             complement(join(398..1468,1470..1559))
                     /gene="prfB"
                     /locus_tag="VFZ86_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564598.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEX5640939.1"
                     /translation="MSPTASEALVHLDELRERLKEASGRLGWLRAIFDPPRLTEQIEA
                     LEERMRAPDFWDDQEHASKVSAEYSRAKRRLEDFAALEARFADLETTEELVREELSGD
                     AVDEELLQELVSGVAALEADLQRGEELRFFSGKYDEGGAILTIHPGAGGTESQDWAEM
                     LLRMYLRWFERRGFKVEVNDLQDGDEAGLKSATLTVEGENVYGLLQAERGVHRLVRLS
                     PFDSANRRHTSFVSVDASPLVSDDVDVEIDEDDLRIDTYRSSGAGGQHVNKTSSAIRI
                     THIPTNIVVQCQNERSQLQNKTVAMHMLKSKLVELEERKRAEELARERGEVLNVDFGS
                     QIRSYVLHPYQMVKDTRTGHEVGNAQSVLDGDLDGFVHAYLVWRAGGSEARK"
     gene            complement(1626..>3693)
                     /locus_tag="VFZ86_01235"
     CDS             complement(1626..>3693)
                     /locus_tag="VFZ86_01235"
                     /inference="COORDINATES: protein motif:HMM:NF019155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HEX5640940.1"
                     /translation="VQRGHHYCIVDEVDSILIDEARTPLIISGAPETAADTYRQFARV
                     VPRLIPGEDYEVDEKQRTVAVTESGVAKVERALDIDNLYKDSNGALVNHFIQALRAQA
                     LYQKDVEYVVQNGEVLIVDEFTGRILEGRRYSEGLHQAIEAKEKVPIREENQTLATIT
                     LQNYFRMYDVLAGMTGTAKTEEDEFRQIYQLEVVQIPTNSPMVRLDENDYIFTTEKEK
                     YAAVVEDVVQRYEKGQPVLVGTVSVEVSEMLSRLLERHGVEHNVLNAKQHEREAHIIE
                     NAGQKGAITIATNMAGRGVDIKLGEGVIEAGGLYVLGTERHESRRIDNQLRGRSGRQG
                     DPGESRFYLSAEDELIRLFAGDRMFKILSRLGPSEGQPIEAKMLSNVVEKAQVKVEEL
                     NFMRRKNVLKYDEVMNEQRRVIYDQRQRILMGEDFGEQVREMVAELVEATVRGAIEGE
                     QYSEDWDLDSLIIGLKQLYNPVLKKTDIDLDGATVEEVVDLAVDDALGQYDERERLIG
                     ADPMRSVERAVMLQVIDGRWKDHLLDMDYLQEGIHLRALGQRDPLVEYKGEGFDLFND
                     MLESVKMSTVTTLMKNSPEDLAMFTAITLDEPVRALNYTSGDDLINQTSFTGAAMAAG
                     DLPEADDGLADRPLPNVAAGGGGRGSATATQPVQVQQRVVYDKVGRNDPCPCGSGKKY
                     KKCHGA"
BASE COUNT          483 a         1247 c         1288 g          675 t
ORIGIN      
        1 cgcgttctcg agcgccgggt cgaccgtgag gcggtggtgc ccgcgcgcgt ccacgagcca
       61 agccgcgagc gtgcgcatgg cgtccggacc gaccccgcgg ccctgggcgg ccgtgccgac
      121 gaagatgtcc acggccgccg agaagtagtc ggggtcggtc tcctccgagt actggatgca
      181 gccggccacc gtggcgtccg gcagcaggat ggtgagcgtg accgcgccga cctcgtcgac
      241 gcgctcccac tcggcccgcc gccaccagcg cgcgacctcg ggctcgtcga ggatgtccag
      301 cagcgccggc aggtcggcgg gcgcgacggg ccgcagggtc acgagggcgc cgcgcagctc
      361 ggagggagtt tcggagccgg cgtccacctc gagggagcta cttgcgcgct tcgctgccgc
      421 cggcgcgcca gaccaggtac gcgtgcacga agccgtcgag atcgccgtcg agcacgctct
      481 gcgcgttacc gacctcgtgg ccggtgcgcg tgtccttcac catctggtag gggtgcagca
      541 cgtagctgcg gatctggctg ccgaagtcca cgttgagcac ctcgccgcgc tcgcgcgcca
      601 gctcctccgc ccgcttgcgc tcctcgagct cgaccagctt cgacttgagc atgtgcatgg
      661 ccacggtctt gttctgcagc tgggagcgct cgttctggca ctgcacgacg atgttggtcg
      721 ggatgtgcgt gatgcggatg gcggagctcg tcttgttgac gtgctggccg ccggcgccgc
      781 tcgaccggta ggtgtcgata cggaggtcgt cctcgtcgat ctcgacgtcg acgtcgtcgc
      841 tcacgagcgg cgaggcgtcg acggagacga agctggtgtg gcggcggttg gcggagtcga
      901 agggactcag gcgcacgagc cgatggacgc cgcgctcggc ctgcaggagc ccgtacacgt
      961 tctcgccctc gacggtgagc gtggcgctct tgagccccgc ctcgtcaccg tcctgcaggt
     1021 cgttgacctc gaccttgaag ccgcggcgct cgaaccagcg caggtacatg cgcagcagca
     1081 tctcggccca gtcctgcgac tcggtgccgc cggcgcccgg gtggatggtg aggatcgcgc
     1141 cgccctcgtc gtacttgccg ctgaagaagc ggagctcctc gccgcgctgc agatcggcct
     1201 cgagggcggc gacgccgctc acgagctcct gcaggagctc ctcgtcgacg gcgtcgccgg
     1261 acagctcctc acgcacgagc tcttcggtgg tctcgaggtc ggcgaaccgg gcttcgagcg
     1321 cggcgaaatc ctcgagccgg cgcttggcgc ggctgtactc ggcggagacc ttggaggcgt
     1381 gctcctggtc gtcccagaag tcgggggcgc gcatgcgctc ctcgagcgcc tcgatctgct
     1441 ctgtcagcct gggggggtca aagatagctc cgcagccagc cgaggcgccc tgaggcttcc
     1501 ttcagccgct cgcggagctc atccaggtga acgagtgctt ctgaggcggt gggactcatg
     1561 accgaggtag tgtacccgaa tcgccgcggc gcttcagccc gacgccgcgg gaacggcggg
     1621 cgcactcagg cgccatggca cttcttgtac ttcttgccgc tcccgcacgg gcacgggtcg
     1681 ttgcgcccga ccttgtcgta cacgacccgc tgctgcacct gcacgggctg cgtggccgtg
     1741 gcggatccgc ggccgccgcc gccggcggcc acgttgggca gcggccggtc ggccaggccg
     1801 tcgtccgcct cagggagatc gccggcggcc atcgccgcgc cggtgaacga cgtctggttg
     1861 atgaggtcgt cgccgctcgt gtagttgagc gcccgcaccg gctcgtcgag tgtgatggcg
     1921 gtgaacatcg cgaggtcctc gggcgagttc ttcatgagcg tcgtgaccgt gctcatcttc
     1981 acgctctcga gcatgtcgtt gaagaggtcg aagccctcgc ccttgtactc gacgagcggg
     2041 tccctctgcc ccagcgcccg caggtggatg ccctcctgca ggtagtccat gtcgaggagg
     2101 tggtccttcc agcggccgtc gatgacctgg agcatgacgg cgcgctccac gctgcgcatc
     2161 gggtcggcgc cgatgaggcg ctcgcgctcg tcgtactggc cgagcgcgtc gtccaccgcg
     2221 agatcgacga cctcctcgac ggtcgcgccg tccaggtcga tgtcggtctt cttgagcacg
     2281 gggttgtaga gctgcttgag cccgatgatc agggaatcga ggtcccagtc ctcggagtac
     2341 tgctcgccct cgatggcgcc gcgcaccgtc gcctcgacga gttccgccac catctcgcgc
     2401 acctgctcgc cgaagtcctc gcccatgagg atgcgctggc gctggtcgta gatgacgcgc
     2461 cgctgctcgt tcatgacctc gtcgtacttg aggacgttct tgcggcgcat gaagttgagc
     2521 tcctcgacct tgacctgcgc cttctcgacc acgttgctca gcatcttcgc ctcgatcggc
     2581 tggccttccg acggcccgag ccggctgagg atcttgaaca tgcggtcgcc ggcgaacagg
     2641 cggatgagct cgtcctcggc ggagagatag aaccggctct cgccggggtc gccctggcgg
     2701 ccggaccggc cgcggagctg gttgtcgatg cggcggctct cgtggcgctc ggtgccgagc
     2761 acgtacaggc cgccggcctc gatcacgccc tcgccgagct tgatgtcgac gccgcggccg
     2821 gccatgttgg tggcgatcgt gatcgcgccc ttctggccgg cgttctcgat gatgtgcgcc
     2881 tcgcgctcgt gctgcttggc gttgagcacg ttgtgctcga cgccgtggcg ctcgagcagg
     2941 cgcgagagca tctcgctgac ctcgacggac acggtgccga cgagcaccgg ctggcccttc
     3001 tcgtagcgct gcacgacgtc ctcgacgacg gccgcgtact tctccttctc ggtcgtgaag
     3061 atgtagtcgt tctcgtccag gcgcaccatc gggctgttgg tcgggatctg cacgacctcg
     3121 agctggtaga tctggcggaa ctcgtcttcc tcggtcttgg cggtaccggt catgcccgcc
     3181 agcacgtcgt acatgcggaa gtagttctgc agggtgatcg tcgcgagcgt ctggttctcc
     3241 tcgcggatcg gcaccttctc cttggcctcg atggcctggt gcaggccctc tgagtaccgc
     3301 ctgccctcga ggatgcggcc cgtgaactcg tcgacgatga gcacctcgcc gttctggacg
     3361 acgtactcga cgtccttctg gtagagggcc tgcgcccgca gcgcctggat gaagtggttg
     3421 accagggcgc cgttggagtc cttgtagagg ttgtcgatgt cgagggcgcg ctcgaccttg
     3481 gcgacgccgc tctcggtgac ggcaacggtg cgctgcttct cgtccacctc gtagtcctcg
     3541 ccggggatga gccgcgggac gacgcgcgcg aactggcggt acgtgtcggc ggcggtctcc
     3601 ggcgcgccgc tgatgatgag cggggtgcgc gcctcgtcga tgaggatcga gtcgacctcg
     3661 tccacgatgc agtagtggtg gccgcgctgc acc
//
LOCUS       DASUMX010000027         2636 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_150940, whole genome shotgun sequence.
ACCESSION   DASUMX010000027 DASUMX010000000
VERSION     DASUMX010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150940"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            140..301
                     /locus_tag="VFZ86_01240"
     CDS             140..301
                     /locus_tag="VFZ86_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5640941.1"
                     /translation="MLRTRQIILVVTAVLGVLLIVRGAWGGVWPLSIQLIAGVLLLVY
                     AGLRWWTMR"
     gene            321..2522
                     /locus_tag="VFZ86_01245"
     CDS             321..2522
                     /locus_tag="VFZ86_01245"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5640942.1"
                     /translation="MARRKHREGESLQKVLGVPALFSTAYGNVGSSIYYALGLVALWA
                     LGVTPVVFLLTGLLFCTTAWSYAEATAMVPEAGGSSSFGRRAFNEFVSFGAGWALMLD
                     YIVTVAISAYFVPNYLAVFWPALKTQPYNSIGGIVTIIALVGINVVGIKEAARLNIVL
                     ALLDLGTQILLMVMGVVLLLEPKLLIEQVQLGVAPTWSHLIYGISIGTIAYTGIETVS
                     NMSEEAANPDRDVPRAINLVLIAVLVVYLGISMTALSAMPVKPNVLPVDATTGQTVPV
                     EVVAKSADEPNGPFVFKGAPPPGNAGNDIYVPAEEEGGRWVIPAQDPAEETFTQGGQT
                     YTRVYGTILGSVYKEDPVVGIVRFLPDDLGWLKAILMPWVGILAATILLIATNAGLIG
                     VSRLAYSLGQHRQVPPILGRVHPKRLTPYIAIIAFGTVACVLLLLPGETISLLADLYA
                     FGAMISFTVAHISVIWLRHTEPDTPRPFRTPLNIDIGRMSIPVLAVIGGLGTFTVWCV
                     VVATHPFGRTIGFIWMGVGLLMYVAYRKAKGYSLTKTLDKVVVPVSMQADIDYHQILV
                     PVTGSRISDEMMVLACQLATEKKSAIDGLYVIEVPLNLPLDASLVSERAKADKVMKAA
                     ALIASQFKVKFTPHVVTARQAGRAIVEEADMRRSEVVVLGTTRKRRIGNLTFGRTTDY
                     VLDHAPCEVLLNLVPRDYPTEGSSVMDGLEQRGPTGPVASGDLKAGPSTKE"
     gene            2533..>2636
                     /locus_tag="VFZ86_01250"
     CDS             2533..>2636
                     /locus_tag="VFZ86_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012921371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEX5640943.1"
                     /translation="MHVVIAGCGRVGSGLARDLVTQGFDVSIIDESPDA"
BASE COUNT          400 a          941 c          849 g          446 t
ORIGIN      
        1 cgcccgccgg cgctctcacc cgctcggtct ccgccttaag acggagtgac cgactcctgc
       61 gtttgtcgga aggtgcgggc gggtgtacat tgagtttcca gaagccggct cgcctacggc
      121 gggccggctt cctgctgaca tgctgagaac cagacagatc atcctcgtcg tcaccgccgt
      181 cctcggcgtc ctgctgatcg tccgcggtgc ctggggcggc gtctggccgc tctccatcca
      241 gctcatcgcc ggcgtgctgc tgctcgtcta cgccgggctg cgctggtgga cgatgaggta
      301 gcggaagagg gcgggggagc gtggcgcgca gaaagcatcg cgagggcgag agcctgcaga
      361 aggtcctcgg cgttcccgcg ctcttcagca ccgcctacgg caacgtaggc tcctccatct
      421 actacgcgct cgggctcgtc gcgctctggg cgctgggcgt cacccctgtc gtcttcctgc
      481 tgaccggcct gctgttctgc accacggcct ggtcctacgc cgaggcgacg gccatggtgc
      541 ccgaggccgg cggctcgtcc agcttcggcc gccgcgcctt caacgagttc gtcagcttcg
      601 gcgccggctg ggcgctgatg ctggactaca tcgtcaccgt cgccatcagc gcctacttcg
      661 tgcccaacta cctcgccgtc ttctggccgg cgctcaagac gcagccgtac aactccatcg
      721 gcggcatcgt caccatcatc gccctggtgg gcatcaacgt ggtgggcatc aaggaggccg
      781 cgcgcctcaa catcgtcctc gcgctgctcg atctgggcac gcagatcctc ctcatggtca
      841 tgggcgtggt gctgctgctc gagccgaagc tgttgatcga gcaggtgcag ctcggcgtcg
      901 cgcccacctg gtcgcacctc atctacggca tctccatcgg caccatcgcc tacaccggca
      961 tcgagaccgt ctcgaacatg tccgaggagg ccgccaaccc ggaccgcgac gtgccccgcg
     1021 ccatcaacct cgtgctgata gccgtgctcg tggtgtacct cggcatctcc atgactgcgc
     1081 tctcggccat gccggtcaaa cccaacgtgc ttcccgtgga cgccacgacc ggtcagacgg
     1141 tgccggtcga ggtcgtggcc aagagcgcgg acgagcccaa cggtcccttc gtcttcaagg
     1201 gcgccccccc gccgggcaac gccggcaacg acatctacgt gccggccgag gaggagggcg
     1261 gcaggtgggt catcccggcg caggaccccg ccgaggagac gttcacccag ggcggacaga
     1321 cgtacaccag ggtgtacggc accatcctgg gcagcgtgta caaggaggac ccggtcgtcg
     1381 gcatcgtgcg ctttctgccc gacgacctcg gctggctcaa ggcgatcctc atgccgtggg
     1441 tcggcatcct cgccgccacg atcctcctga tcgccaccaa cgcggggctc atcggcgtct
     1501 cgcggctggc gtactcgctc gggcagcacc gccaggtgcc gccgatcctc ggtcgcgtgc
     1561 accccaagcg gctcaccccg tacatcgcca tcatcgcctt cgggaccgtg gcgtgcgtcc
     1621 ttctgctgct gccgggcgag accatcagcc tcctcgccga cctgtacgcg ttcggcgcca
     1681 tgatctcgtt cacggtggcg cacatctccg tcatctggct gcggcacacc gagccggaca
     1741 ccccgcgtcc gttccgcacg ccgctcaaca tcgacatcgg caggatgtcg atcccggtgc
     1801 tcgcggtcat cggcgggctc ggcacgttca cggtctggtg cgtcgtcgtg gcgacgcatc
     1861 ccttcggccg caccatcggc ttcatctgga tgggcgtcgg cctcctcatg tacgtggcct
     1921 accgcaaggc caagggctac tcgctcacca agacgctcga caaggtcgtc gtgcccgtga
     1981 gcatgcaggc cgacatcgac taccaccaga tcctcgtgcc cgtcacgggc tcgcgcatca
     2041 gcgacgagat gatggtgctc gcctgccagc tggcgacgga gaagaagtcg gccatcgacg
     2101 gcctgtacgt gatcgaggtg ccgctcaacc tgccgctcga cgccagcctc gtcagcgagc
     2161 gcgccaaggc cgacaaggtc atgaaggcgg cggcgctcat cgcctcgcag ttcaaggtca
     2221 agttcacccc gcacgtggtc acggcgcgcc aggccgggcg ggcgatcgtc gaggaggcgg
     2281 acatgcgccg cagcgaggtg gtcgtgctcg gcacgacgcg caagcggcgc atcggcaacc
     2341 tgaccttcgg gaggaccacg gactacgtgc tcgaccacgc gccgtgcgag gtgctcctca
     2401 atctcgtccc gagggactat cctacggagg gctcgagcgt catggacggg ctcgagcagc
     2461 gggggccgac cggtcctgtc gcctccggcg acctcaaggc cggcccaagc accaaggagt
     2521 gaggagactg caatgcatgt cgtgatcgcc ggctgcggcc gtgtggggtc gggcctggcc
     2581 cgtgacctgg tgacccaggg gttcgacgtc tcgatcatcg acgagagccc ggacgc
//
LOCUS       DASUMX010000028         2033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_151309, whole genome shotgun sequence.
ACCESSION   DASUMX010000028 DASUMX010000000
VERSION     DASUMX010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151309"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(264..1610)
                     /locus_tag="VFZ86_01255"
     CDS             complement(264..1610)
                     /locus_tag="VFZ86_01255"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HEX5640944.1"
                     /translation="MAFAENWDKLTPEERFEKRFEVWQNPDIEFASDAVKAEYQERVQ
                     MFKDAVQLKKPSRPPIAPWIALFPGLDAGLTAKETYYDFDKLHDAWTRFHEIYRPDVL
                     AFTINIAPVKVYDALDYKLYDWPGHGVDEDSGYQYNEAEYMAADEYDKLIADPSNYWQ
                     RFYLPRVIGAMEPWATLDPFTDLVEGPMVGPFFIPFGTPPVQDMLKKMMAAGNAALEW
                     VQAMTKMDQDSMARFGIPGFAGGATKAPYDILGDTMRGTRGLMLDKFRQPDKVLEACE
                     RLVPLAIDWGVRPCDINKHPFVFIPLHKGADGFLSDEDFRTFYWPSFKKVLLGLIDQG
                     IVPLCFAEGGYNDRLEAIHDPEIPAGKMIWMFDATNMQEARKHLGGYQCIGGNVPGAL
                     LTTGTVQDMDDYVRQLMEDVAGDGGFILGSGIVIDAAKPECVKAMIDAGRKYGVQA"
     gene            complement(1610..>2033)
                     /locus_tag="VFZ86_01260"
     CDS             complement(1610..>2033)
                     /locus_tag="VFZ86_01260"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HEX5640945.1"
                     /translation="AKLKPHLVGSAPEEKLGVIVIGTVQGDIHDIGKDIVATMLDIAG
                     FDVVDLGVDVKVARFVATAREKGARIIAMSCLLTSAIDAMKSVVAAAGEAGIRGDVKL
                     MIGGAPITGQVVAYTGADGFGKDAVEAVELAKQWIGGA"
BASE COUNT          290 a          690 c          694 g          359 t
ORIGIN      
        1 ccggagacgc cgtcctcacc gtcaacgtca tcgtcttcgg cgtggacgaa gtggcgctgg
       61 cgacgatcgt gaccggcgcc tggtcgcgcg ccggcgacgc cgagaccgtc tggcgcggct
      121 gagcctcctc cccactcccc gaaaacgaac ggcggcgggg gcctgggcgg gcccccgccg
      181 ccgtcttcgc ttctgcggtc tgagcgcgct cagactcgct gcgcgatcgt cacgggcgcc
      241 cgcccgtcac cgccgcgcgg atgtcacgcc tgcacgccgt acttcctgcc ggcgtcgatc
      301 atggccttga cgcactcggg cttcgccgcg tcgatgacga tgcccgagcc gaggatgaac
      361 ccgccgtcgc cggcgacgtc ctccatgagc tggcgcacgt agtcgtccat gtcctgcacc
      421 gtgccggtgg tgagcagggc gcccggcacg ttgccgccga tgcactggta gccgccgagg
      481 tgcttcctgg cctcctgcat gttggtggcg tcgaacatcc agatcatctt gccggcgggg
      541 atctccgggt cgtggatggc ctcgagacgg tcgttgtagc cgccttcggc gaagcagagc
      601 ggcacgatgc cctgatcgat gaggccgagc aggaccttct tgaagctcgg ccagtagaac
      661 gtgcggaagt cctcgtcgct gaggaacccg tcggcgccct tgtgcagcgg gatgaacacg
      721 aaggggtgct tgttgatgtc gcacgggcgt acgccccagt cgatcgccag cggcacgagc
      781 cgctcacagg cctcgagcac cttgtccggc tggcggaact tgtcgagcat cagcccgcga
      841 gtgccgcgca tggtgtcgcc gaggatgtcg tagggggcct tggtggcgcc gccggcgaag
      901 ccggggatgc cgaacctggc catggagtcc tggtccatct tcgtcatcgc ctggacccac
      961 tccagcgccg cgttgccggc cgccatcatc ttcttcagca tgtcctgcac gggcggcgtg
     1021 ccgaagggga tgaagaacgg gccgaccatc ggaccctcga ccaggtcggt gaaggggtcg
     1081 agcgtggccc acggctccat cgcgccgatc acgcgcggca ggtagaagcg ctgccagtag
     1141 ttggaggggt cggcgatgag cttgtcgtac tcgtcggcgg ccatgtactc ggcctcgttg
     1201 tactggtagc ccgagtcctc gtcgacgccg tggccgggcc agtcgtagag cttgtagtcg
     1261 agggcgtcgt acaccttgac cggggcgatg ttgatggtga aggcgagcac gtccggccgg
     1321 tagatctcgt ggaaacgcgt ccaggcgtcg tgcagcttgt cgaagtcgta gtacgtctcc
     1381 ttggcggtga gacccgcgtc gaggcccggg aacagggcga tccagggggc gatgggcggg
     1441 cgcgacggct tcttgagctg caccgcgtcc ttgaacatct gcacgcgctc ctggtactcc
     1501 gccttgacgg cgtcggaggc gaactcgatg tcggggttct gccacacctc gaaccgcttc
     1561 tcgaagcgct cctcgggggt cagcttgtcc cagttctcgg cgaacgccat cacgcacccc
     1621 cgatccactg cttggcgagc tcgacggctt ccacggcgtc cttgccgaag ccgtccgcgc
     1681 cggtgtaggc gacgacctgc ccggtgatgg gcgcgccgcc gatcatcagc ttgacgtcgc
     1741 cgcggatgcc ggcttcgccc gccgccgcga ccacgctctt catcgcgtcg atggcgctgg
     1801 tgagcaggca gctcatggcg atgatccgcg cgcccttctc ccgcgccgtg gcgacgaacc
     1861 tggcgacctt gacgtccacg ccgaggtcga ccacgtcgaa cccggcgatg tcgagcatcg
     1921 tcgcgacgat gtccttgccg atgtcgtgga tgtcgccctg gacggtcccg atcacgatga
     1981 cgccgagctt ctcctccggc gccgagccga ccagatgcgg cttcagcttc gcg
//
LOCUS       DASUMX010000029         9632 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_2021191, whole genome shotgun sequence.
ACCESSION   DASUMX010000029 DASUMX010000000
VERSION     DASUMX010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 9632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9632
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2021191"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1506)
                     /gene="glyS"
                     /locus_tag="VFZ86_01265"
     CDS             complement(<1..1506)
                     /gene="glyS"
                     /locus_tag="VFZ86_01265"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEX5640946.1"
                     /translation="MNSEHSQAVEPGDEGTPLSTLLVEIGTEELPYKVCESVIRQLEG
                     TPEQPGLALSLLGEQRLLGDGAAARVLVSPRRIAVLVDGVPGEQLAQVQKFRGPKAAI
                     AFADDGTLTKAGAGFARSKGAAAEDVRREVSDGTEFAVVSVEAERRAARDVVPELVAR
                     LVTGLQIPRGMRWGARPADASEYLRFSRPIRWLVAKLGDETLSGSFYGLPLGDLSQGH
                     RVLGAPVAVARAEEYESLLEAEKVVVSQAERRRRITEGLDAAAEELGGAWSDPGGVLA
                     EAVFLAEWPSVARGAFDERHLRLPDEVLVTAMQSHQRYFPVRDRAGDLLPAFLYVSNG
                     DPAAAPLVTRGNERVLDGRLADAEFAYDRDLAEGLPAMAGRLGAVVFHEKLGSLADKA
                     NRIGGLAAALSAGAGAGPGGNGSGDSLAEALRTAARLAKADLVSQTVIEFPVLQGVMG
                     GLYAEAGGLGDAVARAVGEHYRPLSATAPLPGTLAGALLAVADKLDNIAGAW"
     gene            complement(1503..2453)
                     /locus_tag="VFZ86_01270"
     CDS             complement(1503..2453)
                     /locus_tag="VFZ86_01270"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009078520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEX5640947.1"
                     /translation="MTDLAPTAAASSVTTPTFQQIIGRLQAFWEGQGCVVVQPYHTEV
                     GAGTFNPATFLRCLDARPWRAAYVEPSMRPADGRYGENPYRLGHYYQYQVVIKPSPAD
                     IQDVYLRSLEALGIVLADHDVRFVEDNWEGPTLGAWGQGWEVWVDGMEATQFTYFQQL
                     GGFDLDPITIEITYGLERLAMYLQGVDSVYDLVWSRWEEDGATHVVTYGDVYQENERE
                     FSRYSFEVADTEMLYRHFREHEAEALRAIEARLPIPAYDHVLKCSHAFNMLDARGVIS
                     VTDRTAHIARVRDLARRVAGLYLEVVGDGREAPSDGEADG"
     gene            complement(2516..3250)
                     /gene="recO"
                     /locus_tag="VFZ86_01275"
     CDS             complement(2516..3250)
                     /gene="recO"
                     /locus_tag="VFZ86_01275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEX5640948.1"
                     /translation="MRTEKARSVVLSSRPLGEADRIVRLFTRELGRVDAVVKGVRKTT
                     SRWGGRLEPFNVCDLVLHPGRSLYTVTQAQLVDVFARLRADREGLTGAAIVCEAAAGL
                     TPEHEPEERVFALLRNTLRELDRGISGRAVDSPLVLGALLKLLYEAGYLPVLEHCAAC
                     GSGGRALGFSAARGGLVCGDCSGEAVPITPEAIDALAEALARPLAELREQPASAATGE
                     ALRDVHQLYAYHTGSRLRALRIARGV"
     gene            complement(3252..4145)
                     /gene="prmC"
                     /locus_tag="VFZ86_01280"
     CDS             complement(3252..4145)
                     /gene="prmC"
                     /locus_tag="VFZ86_01280"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEX5640949.1"
                     /translation="MTAADALREAGRRLRDADVTRSPRLDAELLLAKVLGVTRAELFR
                     EPGRVLAADEAAEFEALLSRRLVREPVAYILGARAFRTIDLEVTPDVLIPRPETETLV
                     DVALEALAATPIAGPDPEDEPLALDVGTGSGCVALALVAEDPFVRLVATDVDPGALAV
                     ARRNAARLGLARRVEFVLSDLFADVGERPFDLIVSNPPYIPADEYVALEPNVRDYEPR
                     LALYGGVDGLDVYRRLVPGAALLLRPGGTLALEVGAGQADEVAGIVEASGAYAAPERR
                     SDLSGVPRVVFARRRGAKGGG"
     gene            complement(4159..4572)
                     /locus_tag="VFZ86_01285"
     CDS             complement(4159..4572)
                     /locus_tag="VFZ86_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018283650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX5640950.1"
                     /translation="MGLLVLDCSVTMSWLFREEFTPFAERILDEVTASRAVVPSLWPL
                     EVGSVLLQAERRGRVSEADGSRFLALLGGLQIDVDALRGLDLLHDVVPLARATGLTAY
                     DASYLELAARFGVPLATLDRRLAAAASRVGVALCE"
     gene            complement(4573..4812)
                     /locus_tag="VFZ86_01290"
     CDS             complement(4573..4812)
                     /locus_tag="VFZ86_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEX5640951.1"
                     /translation="MTTVGAYEAKTHLAELLKRVENGEHITIARHGTPVAVLVPAGDY
                     PRRDPAAAAAALKEFARGRSRGDFDIRAAIEEGRA"
     gene            complement(4894..5979)
                     /gene="prfA"
                     /locus_tag="VFZ86_01295"
     CDS             complement(4894..5979)
                     /gene="prfA"
                     /locus_tag="VFZ86_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEX5640952.1"
                     /translation="MATIHDLISEIERSYDELNAQLADPEVLGDRARYADAARAHAEL
                     QQVHELTAQYREAERTVADAEGLLGDDSSDAEMREFAQDELVDGRRRLDELAEEIRVR
                     MLTRDPNDGKDVIIEIRAGTGGNEACIFAGELARMYTRYAQAHGFEVEMLAANENDDG
                     GYKEVTLEVKGQGAYSTLKYEGGVHRVQRVPVTEAAGRIHTSTATVAVLPEAEDIEVD
                     VQAGDLRVDIFRARGPGGQSVNTTDSAVRITHVPTGVSVSCQDEKSQLQNKEKAMRIL
                     RARLFELEQARQDRERGAARRSMVGSGDRSQKIRTYNFQQNRVSDHRIGLTSHRLTEV
                     MEGDLDEFTEALAAEDRRKQLAAAGGE"
     gene            complement(5989..6942)
                     /locus_tag="VFZ86_01300"
     CDS             complement(5989..6942)
                     /locus_tag="VFZ86_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005383586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HEX5640953.1"
                     /translation="MIRRLALIALLLPALAAKRAQVGGQAVIEGVMMRGVDWWSLAVR
                     RPDDSIGVHHYPLVSLMKRYPVLKLPVLRGMIALFESMVIGIRALMQSANESLGEAEE
                     ELGKKEIAVTLTIAFAFAIGLFFIAPLFLTGLMERWLGEGFLFWMVEGFVRVGIFLVY
                     LAIVTQIKDLRRVFEYHGAEHMSIHALEHGEELTVDNVVKYETLHLRCGTSFLLIVLV
                     VSIFVFAAVGRPPWYLLILSRVVFVPLIAGISYEIIRFAGRREGSKAVRIIMAPGLAL
                     QWMTTKKPDRAQVEVAIAALEKIIELEPQDRPPQKGVEVMA"
     gene            complement(6972..7760)
                     /gene="thyX"
                     /locus_tag="VFZ86_01305"
     CDS             complement(6972..7760)
                     /gene="thyX"
                     /locus_tag="VFZ86_01305"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009304741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HEX5640954.1"
                     /translation="MNSPRSDAIERGPGGSPLRATLRVGLLQHTPDPDRAVAIAGRLC
                     YAPVSAADLKQEMSGEDVAKLVRILVRSGHHSALEHATFSFAVDGVSRACTHQLVRHR
                     VASYNQQSQRYVNFGAADSFVVPPSISANAEAEAVFLRAMEDARVAYDTLVELGLAEG
                     RTRESVQEDARFVLPNAAETKIVVTMNARELRHFFQVRCCNRAQWEIRDLAWQMRAMV
                     VELAPNLFARSGPGCLYGTCPEGKMTCGKPYKADEVDGPEAARR"
     gene            complement(7768..8091)
                     /gene="rpmE"
                     /locus_tag="VFZ86_01310"
     CDS             complement(7768..8091)
                     /gene="rpmE"
                     /locus_tag="VFZ86_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEX5640955.1"
                     /translation="MKKDIHPQYEEATVHCSCGNTFTTRSTKKDLHVELCSQCHPFYT
                     GKQKLVDSGGRVDRFKRRLEKKADEPVKPKAPRARAPKVELPKPEPVAVAAESASEAE
                     KTDES"
     gene            8333..9328
                     /locus_tag="VFZ86_01315"
     CDS             8333..9328
                     /locus_tag="VFZ86_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HEX5640956.1"
                     /translation="MPDMFAHYLVAEAAAQRADAGPLLAGSFDAYKVGAQGPDVFFYS
                     RLVRGRRGRPNLAHLTHQYQMAAAFRSMLTRAAASPAEERGAAFAFVAGYAAHLCLDA
                     GAHPWVLYWTGDITDGADPPARTEAFRRHGILEASIDVILTRERSDDTAWLRRQRLLR
                     LPPAQAMTAARLLSGMLSDVHGVTFTPGEGRSAFRAMEWVYTTMSDPRSLPTRLLGMV
                     APAVDKGGVVRTQIYPETPAPAAAGLYAERSPWYYPSLPEEPRTATFAEILETATAET
                     ARCLRAIVAVADRDAAVDEAVAVIGDRSMFTGVACGDPRPLVAFAPGRKELWGEA"
     gene            complement(9338..>9632)
                     /locus_tag="VFZ86_01320"
     CDS             complement(9338..>9632)
                     /locus_tag="VFZ86_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018544503.1"
                     /note="An RNA-DNA helicase that actively releases nascent
                     mRNAs from paused transcription complexes; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEX5640957.1"
                     /translation="SRMDEVIFEEFKGTGNMEIRLDRKLADKRIFPAIDIDATGTRKE
                     ELLMEPAEAAAVWKLRGVLHSLDPAAAIELLIKKIRETKNNAEFLSALIKNSR"
BASE COUNT         1338 a         3434 c         3467 g         1393 t
ORIGIN      
        1 ccaggcgccg gcgatgttgt cgagcttgtc ggcgacggcg agcagcgcgc cggcgagggt
       61 gccgggcagc ggggccgtcg cggagagcgg ccggtagtgc tcgccgacgg cgcgggccac
      121 agcgtcgccg aggccgccgg cctcggcgta gagcccgccc atgacgccct gcagcacggg
      181 gaactcgatg accgtctggc tgaccaggtc ggccttcgcc aggcgcgcgg ccgtcctcag
      241 ggcctccgcg agggagtcgc cggagccgtt gcctccgggg ccggcgccgg cgccggcgga
      301 cagcgccgcg gccaggccgc cgatccggtt tgccttgtcg gcgagcgacc cgagcttctc
      361 gtggaacacg acggcgccga ggcggccggc catggccggc agcccctcgg cgaggtcgcg
      421 gtcgtaggcg aactcggcgt ccgcgagacg gccgtcgagc acgcgctcgt tgccgcgcgt
      481 gaccagcggc gccgccgccg ggtcgccgtt ggagacgtag aggaaggccg gcaacagatc
      541 gccggcgcga tcgcgcaccg ggaagtaccg ctggtggctc tgcatggccg tcaccagcac
      601 ctcgtcgggc agccgcaggt ggcgctcgtc gaaggcgccg cgggcgacgc tcggccactc
      661 ggccaggaag accgcctcgg cgagcacgcc gccggggtct gaccacgcgc cgccgagctc
      721 ctcggcggcg gcgtcgagcc cctccgtgat gcggcggcgg cgctcggcct gtgagacgac
      781 gaccttctcg gcctcgagca ggctttcgta ctcttcggcg cgcgcgaccg ccaccggcgc
      841 gccgagcacg cggtgaccct gcgagaggtc gccgagcggg aggccgtaga aggacccgct
      901 cagcgtctcg tcgccgagct tggccaccag ccagcggatg ggacgcgaga agcgcaggta
      961 ctcgctcgcg tccgccggcc tcgcgcccca gcgcatgccg cgcgggatct gcaggccggt
     1021 gacgaggcgc gcgaccagct cgggcacgac gtcgcgcgcg gcgcggcgct cggcctccac
     1081 gctgacgacg gcgaactcgg tcccgtcact cacctcacgg cgcacatcct ccgccgccgc
     1141 gcccttggag cgtgcgaacc ccgcgccggc cttggtgagc gtgccgtcgt cggcgaacgc
     1201 gatcgcggcc ttggggccgc ggaacttctg cacctgggcg agctgctctc ccggcacgcc
     1261 gtcgaccagg acggcgatgc gccgcggcga caccaggacc ctggccgccg cgccgtcccc
     1321 cagcagccgc tgctcgccga gcagcgacag cgcgaggccg ggctgctccg gcgtgccctc
     1381 gagctgccgg atcacggact cgcagacctt gtacggcagc tcttcggtgc cgatctcgac
     1441 gagcagcgtg ctgagcggcg tcccctcgtc gcccggctcg acagcctggc tgtgctcgct
     1501 gttcatccgt ccgcctcgcc gtcggaaggc gcctcgcgcc cgtcgccgac gacctccagg
     1561 tacaggccgg cgacccggcg cgcgaggtcg cgcacgcggg cgatgtgcgc cgtgcggtcg
     1621 gtgacgctga tcacgccgcg cgcgtcgagc atgttgaacg cgtggctgca cttgagcacg
     1681 tggtcgtagg cggggatggg caggcgcgcc tcgatggcgc gcagcgcctc ggcctcgtgc
     1741 tcgcggaagt ggcggtacag catctcggtg tccgccacct cgaagctgta gcggctgaac
     1801 tcgcgctcgt tctcctggta cacgtcgccg taggtgacga cgtgcgtggc gccgtcctcc
     1861 tcccagcgcg accacacgag gtcgtagacg ctgtccactc cctgcaggta catggcgagg
     1921 cgttcgaggc cgtaggtgat ctcgatggtg atggggtcga ggtcgaagcc gccgagctgc
     1981 tggaagtacg tgaactgggt ggcctccatg ccgtcgaccc agacctccca gccctggccc
     2041 caggcgccga gcgtgggacc ctcccagttg tcctcgacga agcgcacgtc gtgatcggcg
     2101 agcacgatac cgagcgcctc gagagagcgc aggtagacgt cctggatgtc cgccggcgac
     2161 ggcttgatga ccacctggta ctggtagtag tggccgagcc ggtaggggtt ctcgccgtag
     2221 cgcccgtcgg cggggcgcat ggaaggttcc acgtaggccg cgcgccaggg acgggcgtcc
     2281 aggcagcgca ggaacgtcgc gggattgaag gtgccggcgc cgacctcggt gtgatacggc
     2341 tgcacgacca cgcagccctg cccctcccag aaggcctgca ggcggccgat gatctgctgg
     2401 aacgtgggcg tggtcacgga cgatgcggct gcagtcgggg cgaggtcggt cacggatcct
     2461 ttcggtcgga gcggcgattc taccagtagc tcgcagaagc gaacaaacag gcccttcaga
     2521 cgccgcgcgc gatgcgcagc gcgcgcagcc gtgaccccgt gtggtacgcg tacagctggt
     2581 gcacgtcgcg cagcgcctcg cccgtcgcgg cggaggcggg ctgctcgcga agttcggcca
     2641 gcgggcgggc gagcgcctcc gcgagcgcgt cgatcgcctc cggcgtgatc ggcactgcct
     2701 cgccggagca gtcgccgcac acgaggccgc cccgggcggc cgagaagccc agcgcgcggc
     2761 cgcccgagcc gcaagccgcg cagtgctcga gcaccggcag gtagccggcc tcgtacagga
     2821 gcttgaggag cgcgccgagc acgagcggcg agtcgacggc gcggccggag atgccccggt
     2881 cgagctcgcg cagcgtgttg cgcagcagcg cgaacacgcg ctcctcgggc tcgtgctccg
     2941 gggtgagccc ggcggcggcc tcgcagacga tcgccgcccc cgtgagcccc tcgcggtcgg
     3001 cgcgcagccg cgcgaacacg tccaccagtt gcgcctgcgt gaccgtgtac agcgacctcc
     3061 ccgggtgcag caccaggtcg cagacgttga acggctccag gcggccgccc cagcgggacg
     3121 tggtcttgcg cacgcccttg accaccgcgt cgacgcggcc gagctctcgc gtgaacaggc
     3181 ggacgatacg gtcggcctcg cccagaggac gcgagctcag gacgacggag cgggctttct
     3241 cggtgcgcac ctcagccgcc gcccttggcg ccgcggcggc gcgcgaagac cacccggggc
     3301 acgccggaga ggtcgctgcg ccgctccggc gccgcgtacg ccccggaggc ctcgacgatg
     3361 ccggccacct cgtccgcctg cccggcgccg acctccaggg ccagggtccc gcccggccgc
     3421 agcaggagcg ccgcgccggg gacgagccgg cggtacacgt ccagcccgtc cacgccgccg
     3481 tacagcgcga ggcgcggctc gtagtcgcgg acgttgggct ccagcgccac gtactcgtcg
     3541 gcggggatgt acggcgggtt ggagacgatg aggtcgaacg gccgctcgcc gacgtccgcg
     3601 aacaggtcgg agagcacgaa ctcgacgcgg cgcgccaggc cgaggcgcgc cgcgttgcgc
     3661 cgcgccaccg cgagcgcgcc ggggtccacg tccgttgcca ccaggcgcac gaacgggtct
     3721 tcggcgacga gcgcgagcgc gacgcagccc gagccggtgc ccacgtcgag cgccagcggc
     3781 tcgtcctcgg ggtccggccc ggcgatcggc gtcgccgcga gcgcctcgag cgcgacgtcc
     3841 acgagggtct ccgtctccgg ccgcgggatg agcacgtccg gcgtgacctc gaggtcgatg
     3901 gtgcggaacg cgcgcgcacc gaggatgtag gccaccggtt cgcgcacgag gcggcggctg
     3961 aggagcgcct cgaactcagc cgcctcgtcc gcggcgagca cgcgccccgg ctcgcggaac
     4021 agctcggccc gcgtcacccc cagcaccttg gcgagcagca gctcggcgtc cagacgcggc
     4081 gagcgcgtga cgtctgcgtc ccgcagccgc cggcccgcct ccctcagagc gtctgccgcg
     4141 gtcaaactga gctcactctc actcacagag cgccacgccc acgcgcgagg cggccgcagc
     4201 cagacgcctg tccagcgtcg ccagcggcac gccgaaccgc gcggcaagct ccaggtagct
     4261 cgcgtcgtag gccgtgaggc cggtcgcccg cgccagcggt accacgtcgt gcagcaagtc
     4321 gagaccgcgc agggcatcga cgtcgatctg aagcccgccc aagagggcaa ggaaacgtga
     4381 gccgtccgcc tcgctcaccc ggccgcgcct ctccgcctgc agcaggacgc tgcccacttc
     4441 gagcggccag agcgagggca cgaccgcccg gctcgcggtg acctcgtcca ggatccgctc
     4501 ggcgaacggc gtgaactcct cgcggaacag ccaggacatg gtgaccgagc agtcgaggac
     4561 gagcaggccc actcaggccc ttccttcctc gatcgcggca cggatgtcga agtctcccct
     4621 gctgcgaccg cgcgcgaact ccttgagcgc cgccgcggcc gccgcaggat ccctgcgggg
     4681 atagtcgcct gcgggcacaa gcacggcgac gggcgtaccg tgccgcgcga tggtgatgtg
     4741 ctctccgttc tccacacgct tgaggagttc agccagatgg gtcttcgcct cgtaggcacc
     4801 gacggtcgtc atgatcgtca cctctcagac tggtctgcga ctggtctgag tatacccgag
     4861 gcacgccgcc acgtcacgcg ttcccgggcc gcgtcactcc ccgccggcgg ccgccagctg
     4921 cttgcgccgg tcctccgcgg cgagcgcctc ggtgaactcg tcgagatcgc cctccatcac
     4981 ctcggtgaga cggtggctgg tgagcccgat gcggtggtcg ctgacgcggt tctgctggaa
     5041 gttgtaggtg cggatcttct gtgaacggtc gccgctgccg accatgctcc gccgggcggc
     5101 cccgcgctcc cggtcctgcc tggcctgctc gagctcgaac agccgcgccc gcaggatccg
     5161 catcgccttc tccttgttct ggagctggct cttctcgtcc tggcagctca cggagacgcc
     5221 ggtcggcacg tgcgtgatgc gcacggcgga gtcggtggtg ttcacgctct ggccgccggg
     5281 accgcgagcg cggaagatgt cgacgcgcag atcgccggcc tgcacgtcga cctcgatgtc
     5341 ctcggcctcg ggcagcaccg ccacggtggc ggtggaggtg tggatgcggc ccgcggcctc
     5401 ggtgaccggc acgcgctgca cgcggtgcac gccgccctcg tacttgagcg tcgagtaggc
     5461 gccctggccc ttgacctcga gcgtgacctc cttgtacccg ccgtcgtcgt tctcgttggc
     5521 ggcgagcatc tccacctcga acccgtgggc ctgggcgtag cgcgtgtaca tgcgcgcgag
     5581 ctcgccggcg aagatgcagg cctcgttgcc gccggtgccg gcgcggatct cgatgatcac
     5641 gtccttgccg tcgttggggt cgcgcgtgag catgcgcacg cggatctcct cggcgagctc
     5701 gtcgagccgg cggcggccgt cgacgagctc gtcctgggcg aactcgcgca tctcggcgtc
     5761 ggaagagtcg tcaccgagga ggccttcggc gtcggcgacc gtccgctccg cctcgcggta
     5821 ctgcgccgtg agctcgtgga cctgctggag ctcggcgtga gcgcgcgcgg cgtccgcgta
     5881 gcgcgcgcgg tcgccgagca cctcggggtc cgcgagctgc gcgttgagct cgtcgtacga
     5941 gcgttcgatc tcgctgatca ggtcgtggat ggtggccatg gccccagctt acgccatgac
     6001 ctccacgccc ttctgcggcg gtcggtcctg cggctccagc tcgatgatct tctcgagggc
     6061 ggcgatggcc acctcgacct gggcgcggtc gggcttcttg gtggtcatcc actgcagcgc
     6121 gaggccgggc gccatgatga tgcggacggc cttgctgcct tcgcgtcgtc cggcgaaccg
     6181 gatgatctcg tagctgatgc ccgcgatgag cgggacgaag accacgcggc tgaggatgag
     6241 caggtaccac ggcgggcggc cgaccgccgc gaagacgaag atcgagacca cgagcacgat
     6301 gagcaggaac gacgtgccgc agcgcaggtg cagggtctcg tacttgacga cgttgtcgac
     6361 cgtgagctcc tcgccgtgct cgagcgcgtg gatgctcatg tgctcggcgc cgtggtactc
     6421 gaacacgcgg cgcaggtcct tgatctgggt cacgatggcc aggtagacga ggaagatccc
     6481 gacgcgtacg aagccctcga ccatccagaa gaggaatccc tctccgagcc agcgctccat
     6541 cagcccggtg aggaagagcg gcgcgatgaa gaagaggccg atggcgaagg cgaaggcgat
     6601 cgtgagcgtg acggcgatct ccttcttgcc gagctcctcc tccgcttcgc ccagtgactc
     6661 gttggcggac tgcatgagag cgcggatgcc gatgaccatc gactcgaaca gggcgatcat
     6721 gccgcgcagc acgggaagct tgagcaccgg gtagcgcttc atgaggctga ccagcggata
     6781 gtgatggacg ccgatggaat cgtcggggcg gcgcaccgcc agcgaccacc agtcgacgcc
     6841 ccgcatcatg acgccctcga tgacggcctg cccgcccacc tgggcgcgct tggcggccag
     6901 ggcgggcagg aggagcgcga tgagagcgag acggcggatc atgacagttg cgggccgcgc
     6961 gggtgcggca ctcagcgccg cgcggcctcc gggccgtcga cctcgtcggc cttgtacggc
     7021 ttgccgcacg tcatcttgcc ctccgggcag gtgccgtaca gacagccggg gccgctccgc
     7081 gcgaagaggt tgggcgccag ctcgacgacc atcgcgcgca tctgccaggc gaggtcgcgg
     7141 atctcccact gcgcgcggtt gcagcagcgc acctggaaga agtggcgcag ctcgcgcgcg
     7201 ttcatggtga cgacgatctt ggtctcggcg gcgttgggca gcacgaagcg cgcgtcctcc
     7261 tggacgctct cccgcgtgcg gccctcggcg aggccgagct ccacgagcgt gtcgtaggcg
     7321 acgcgcgcgt cctccatggc ccggaggaag acagcctccg cctctgcgtt ggcggagatg
     7381 ctgggcggca ccacgaagct gtcggccgcc ccgaagttga cgtaccgctg cgactgctgg
     7441 ttgtaacttg cgactcgatg tctgacaagc tggtgcgtgc aggcgcggct cacgccgtcc
     7501 acggcgaagc tgaacgtggc gtgctcgagc gcggagtggt gaccgctgcg gaccaggatg
     7561 cgcacgagct tggcgacgtc ctcgccgctc atctcctgct tgaggtccgc cgccgacacg
     7621 ggcgcataac agagccgacc cgcgatcgcg acggcgcgat cggggtcggg cgtgtgctgg
     7681 aggaggccga cgcggagcgt ggcgcggagc ggtgaccctc cggggccccg ctcgatagcg
     7741 tcgctgcggg ggctgttcat cccggcgtca ggactcgtcg gtcttctcgg cctcggaggc
     7801 ggactcggcg gccacggcca ccggctccgg cttcggcagc tcgaccttgg gcgcgcgtgc
     7861 tctcggcgcc ttcggcttga ccggctcgtc ggccttcttc tcgagacggc gcttgaagcg
     7921 gtccacgcgg ccgcccgagt ccacgagctt ctgcttgccc gtgtagaacg ggtggcactg
     7981 cgagcacagc tcgacgtgca ggtccttctt ggtgctgcgc gtggtgaacg tgttgccgca
     8041 gctgcagtgg acggtcgcct cttcgtactg cgggtggatg tctttcttca tctcaacgga
     8101 tcccttctga gcaaacccgg tagtgacgcg gctggaggcc ccgaagggcc gaagcgggat
     8161 gataccacga accccgcggc cacatcgaat cgttcccgta gccaaacgcg ggcccgggag
     8221 ccgcacgcag cggcgcggat gcagaggcgg cgcattcctc cctatactcc gtgccgacca
     8281 ggccgccggc gcgcactggc gcgcctgcga gacagctcga gaagaggacc ggatgccgga
     8341 catgttcgcc cactatctcg tcgcggaggc cgcggcgcaa cgcgccgacg ccggcccgct
     8401 cctcgccggc agcttcgacg cctacaaggt gggcgcgcag ggcccggacg tgttcttcta
     8461 ctcgcgtctg gtgcgcggtc gccgcgggcg ccccaacctc gcccacctca cgcatcagta
     8521 ccagatggcc gcggcgttcc gcagcatgct cacccgcgcc gcagcctcgc cggcggagga
     8581 gcgcggggct gccttcgcct tcgtcgcggg gtacgccgcg cacctgtgtc tggacgcggg
     8641 ggcccacccg tgggtgctct actggacggg ggacatcacc gacggagctg atccgccggc
     8701 caggaccgag gccttccgcc ggcacgggat cctcgaggcg tccatcgacg tcatcctcac
     8761 ccgcgaacgg tccgacgaca ccgcctggct gcgccggcag cggctcctgc gcctgccgcc
     8821 ggcgcaggcg atgacggccg cgaggctgtt gagcggcatg ctgagcgacg tgcacggcgt
     8881 gacgttcacc cccggggagg gacgctcggc cttccgcgcg atggagtggg tctacacgac
     8941 gatgagcgat ccgcgctccc tgccgacgcg gcttctcggg atggtcgccc ccgcggtcga
     9001 caagggcggc gtcgtgcgca cccagatcta cccggagacc ccggcgcctg ccgccgccgg
     9061 cctctacgcc gagcggagcc cctggtacta cccctcgctg ccggaggaac cgcgcaccgc
     9121 gacgttcgcg gagatcctcg agaccgcgac ggccgagacc gcgcgctgcc tgcgggcgat
     9181 cgtcgccgtg gccgatcgcg acgcggccgt ggacgaggcg gtggccgtca tcggcgaccg
     9241 gagcatgttc accggtgtgg cgtgcggcga tccgcggccg ttggtggcct tcgcccccgg
     9301 gagaaaagag ctctggggcg aggcctgagc gccggcgtca gcgcgagttc ttgatcagcg
     9361 cgctgaggaa ctcggcgttg ttcttggtct cgcggatctt cttgatgagc agctcgatgg
     9421 ccgcggccgg atcgaggctg tgcagcacgc cgcggagctt ccagacggcg gcggcctcgg
     9481 ccggctccat gagcagctcc tccttgcgcg tcccggtggc atcgatgtcg atggccggga
     9541 agatgcgctt gtcggcgagc ttgcggtcga gccggatctc catgttgccg gtgcccttga
     9601 actcctcgaa gatcacttcg tccatgcgcg ag
//
LOCUS       DASUMX010000030         6599 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_2021570, whole genome shotgun sequence.
ACCESSION   DASUMX010000030 DASUMX010000000
VERSION     DASUMX010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 6599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6599
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2021570"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="VFZ86_01325"
     CDS             <1..759
                     /locus_tag="VFZ86_01325"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX5640958.1"
                     /translation="AGARTQVANLVAWVVTIVTLLFLTPLFAPLPEAVLAALIIHALW
                     HLVAARKLVKLRRAAPVEVWFGVITLWGVLLFDVLGGMIIGLLASMLFVVYRSSRARL
                     SSLGRVPGVPGAYSDVARHPDDTPVPGVLIVRFDAPVYYANALTVRDAVRDMIADQEP
                     PPRAVVLDAGTQDELDVTSTEMVKGLVKQLREGGLQVYFADVHAPILDRGRHTGLLET
                     IGEDHVFPTVDAAVRHIEAETEPVKLNAGGRRDD"
     gene            752..1915
                     /gene="gntH"
                     /locus_tag="VFZ86_01330"
     CDS             752..1915
                     /gene="gntH"
                     /locus_tag="VFZ86_01330"
                     /inference="COORDINATES: protein motif:HMM:NF041257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanitoxin biosynthesis MBL fold
                     metallo-hydrolase GntH"
                     /protein_id="HEX5640959.1"
                     /translation="MTDSDLTSLPAPTFAGGTRAITLTPVVGTPRREYAETFVPGEEG
                     LEDGELRVTVLGSGNPWTTRAQASASILVEVGNPEHDLLVFDLGGGSLANYASLKLPV
                     NLLDKVFFSHLHADHTADLITLSGSYSKVGRADGPVRVWGPSGTEPSLGTRHFVEGVR
                     EALAWDTAAGNGAINPDSMRIEVTEFDFSETGVVYERNGVTVTSFPVVHALSGSVGYR
                     LDFAGLSFVYSGDTCAAWPLVRASEGCDLLIHEVFPPAAVLAAASGLSIQRATIALNA
                     AHTSPTAAAKVFSLVKPRMAGLWHTLLNPQIIPMIFTELRAGYDGPVVQTQDLTVFNV
                     TREAVVARQAQVPDQLPPTPGKQRAPFAPAAVAPPEWWAEARIPLDGAGTPGS"
     gene            1912..3123
                     /locus_tag="VFZ86_01335"
     CDS             1912..3123
                     /locus_tag="VFZ86_01335"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX5640960.1"
                     /translation="MRGADENATGAESTEAAGPAGEPSAFAAPRWARDVGLWSWIVVG
                     ALLVLLALFWAAASIQMVVLASLFAVLFGGTFLPVVDWLHKHRLPRWLGALLVLVFLI
                     ALGIFIALVILYGLVKQLPVIQENLQAAFDSISEALASTSVDQSTLDSMKTAIGGVLE
                     FAATGAAGTVASIASGLASLIFGVFISINILVWVLIQGRKIASWASRHVPPVPQSVAY
                     TIFANSARFFRGYIFGSTLVGLFNAAVIFVGALIIGVPMPGTLAIVAWMMNYIPFFGA
                     IVSGAFAVLIAYGAGGLSMAIPMLIIVIIANGFLQTLVSQFALGSTLDLHPLVVLFAT
                     TAGGILFGAVGGVFAAPFVKIGLDAYARVKAAGLFGSGKQSPNAAGASHSPAAEAVDL
                     ESLVVAEGTGS"
     gene            3137..4456
                     /locus_tag="VFZ86_01340"
     CDS             3137..4456
                     /locus_tag="VFZ86_01340"
                     /inference="COORDINATES: protein motif:HMM:NF021524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 family protein"
                     /protein_id="HEX5640961.1"
                     /translation="MGVETGGDAFEGVPAARRLGRTLRRHRRLRITLIQLAYVAGGIA
                     LGLALPRIPVGFTVPRAETTQMLFAVGAGLLGFLAIAFSLAFLVVQFGSTTYTPRLNL
                     FYTSPRIWHGFGFITGVLVFAFAAAYSETFASASGGASDERMSGLVPIVLIALLMGAI
                     VVYRNLQMRAFGSVELGSVLAEVTERGREVLDGMYADEPPREADGPPQQDGEQRGLRA
                     LPEGSREVAWPGRSGIIQDVDVPRIIAAARSADAAVEIVVPTGEMVHHRATVAVIHGS
                     VDPSLDAAVVKAVRTGAGRTFEQDPTMAFRVLVDIALRALSPAINDPTTAVQVLDCEE
                     DLLRMLADRDLDAGEITGPRGRTRVLLPLPSWDDYVALAVDEIVEAGAAQVRIRRRVE
                     CLLRELTALAPESRREPLQARLDELASQWQPAGSGTATTLSGEHPGA"
     gene            4535..5476
                     /locus_tag="VFZ86_01345"
     CDS             4535..5476
                     /locus_tag="VFZ86_01345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX5640962.1"
                     /translation="MATSRTKWWSRARAAVAAAGGPIKEHDLPRHAAALTYYLVLALF
                     PILIVVTALLGVIGLTPEALQSLLDAVAQLGSPWAVEFVRGVLDSVLASSGTPLLLSV
                     GVLMALWTASGYVAAFMWAAAGIGGERDTRRWFSGLAVRLGLALLLAVLLSLAATVVV
                     LATPFAQWLGDLLGIGETAVQVWTTVDWPFFFLVAVAVFLLLYRAAPHSHRRRVLHDL
                     IGACCGVTIWLIASAAFSFYLANFGSYDRVYGVLGTGIAFLVWAWILDLTLLGGLEVS
                     VGLGTWRERPKAKGDAAVPPEPSPLEPRTPHAGGAGR"
     gene            5583..6281
                     /locus_tag="VFZ86_01350"
     CDS             5583..6281
                     /locus_tag="VFZ86_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009476678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5640963.1"
                     /translation="MTELSSSEVTSRKVARRAATPIAGAIAGIIFAVLYTVSMTALRQ
                     TVGDAASDTGAWLTEDTGLVKFALALVPFAGLAFLWFIAVARERLGRFEDQFFSTVFI
                     GSGLLFLAMVFVAAATAGAIVAAAANDPEGFAASTTYTYARQAVTQILGVYAVRMAAI
                     FQLSQASLWLRTHVMPRWMALITIVVALVLLFVFTQSWWVVLVFPAWVLLVSIYILIA
                     QPAQEERAAGERRP"
     gene            complement(6333..>6599)
                     /locus_tag="VFZ86_01355"
     CDS             complement(6333..>6599)
                     /locus_tag="VFZ86_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5640964.1"
                     /translation="AVEFKVVADADLDRVVHVAVEAARATAGASAEVEESFPPTCDVV
                     SLAGAGATCVTRLGIKHYRRRRLAQDAYLRELQKQFLSEGIALG"
BASE COUNT          894 a         2336 c         2279 g         1090 t
ORIGIN      
        1 gcaggcgccc gcacccaggt cgcgaacctg gtcgcctggg tcgtgacgat cgtgaccctg
       61 ctgtttctca cgccgctgtt cgcgccgctc cccgaagcgg tgctggcggc gctgatcatc
      121 cacgcgctct ggcacctcgt cgccgcgcga aagctggtga agctccggcg cgcggcgccg
      181 gtcgaggtct ggttcggggt catcacgctg tggggcgtgc tgctgttcga cgtccttgga
      241 ggcatgatca tcggcctgct cgcctcgatg ctcttcgtgg tctaccggtc cagccgcgcg
      301 cgtctctcct cgctcgggcg cgtgcccggc gtgcccggcg cgtactctga cgtggcccgg
      361 catcccgacg acactccggt gccgggagtc ctgatcgtgc gtttcgatgc gccggtctac
      421 tacgcaaacg ccctgacggt cagagacgca gtgcgcgaca tgatcgcgga ccaggagccg
      481 ccgccccggg ccgtcgtcct tgacgccggc acccaggacg agctcgacgt gaccagcacg
      541 gagatggtca aggggctcgt gaagcagctg cgcgaaggcg gtctgcaggt gtacttcgcc
      601 gacgtgcacg cgcccatcct cgatcgtggc cgccacaccg gtctgctgga gaccatcggc
      661 gaagaccatg tgttcccgac ggtggacgcc gccgtgcgtc acatcgaagc agagacagaa
      721 cccgtgaagc tgaatgcagg aggaagacga gatgactgac agcgacctca catccctgcc
      781 ggcgccgacg ttcgccggcg gcaccagggc gatcaccctg acgccggtcg tcggcacgcc
      841 gcggcgcgag tacgccgaga cgttcgtgcc gggcgaggag gggctcgagg acggtgagct
      901 gcgcgtgacc gtgctcggca gcggcaaccc ctggacgacg cgcgcgcagg cctcggccag
      961 catcctggtc gaggtcggca accccgagca cgacctgctc gtcttcgacc tcggcggggg
     1021 atcgctggcg aactacgcga gcctcaagct gccggtcaac ctgctcgaca aggtgttctt
     1081 cagccacctg cacgccgacc acactgcgga cctcatcacc ctctcgggca gctactcgaa
     1141 ggtggggcga gccgacgggc cggtcagggt ctggggcccc agcggcacgg agcccagcct
     1201 gggcacccgg cacttcgtcg agggggtccg ggaggcgctc gcctgggaca ccgcggccgg
     1261 gaacggcgcc atcaacccgg acagcatgcg catcgaggtc accgagttcg acttcagcga
     1321 gacgggggtc gtctacgagc ggaacggcgt cacggtcacg tcgttccccg tcgtccacgc
     1381 gctcagcggc agcgtcggct accgcctcga cttcgccggc ctctcgttcg tctactcggg
     1441 cgacacctgc gccgcctggc cgctggtgcg cgccagtgaa ggctgcgacc tgctcatcca
     1501 cgaggtcttc ccgccggccg cggtgctggc ggccgcgtcc ggcctctcca tccagcgcgc
     1561 gaccatcgcg ctgaacgcgg cgcacacctc gccgacggcc gccgccaagg tgttcagcct
     1621 ggtcaagccg cgcatggccg gcctgtggca cacgctgctc aacccgcaga tcatcccgat
     1681 gatcttcacg gagctccgcg ccggctacga cggccccgtg gtccagaccc aggacctcac
     1741 ggtgttcaac gtgacccggg aggccgtggt cgcgcgccag gcgcaggtgc cggaccagtt
     1801 gccgccgacc ccgggcaagc agcgcgcgcc gttcgcgccg gcggcggtgg ctcctccgga
     1861 gtggtgggcc gaggcgcgca tcccgctgga cggcgccggg acgcccgggt catgagaggc
     1921 gcggacgaga acgcgaccgg cgcggaatcc acggaggccg ccggcccggc cggcgagccg
     1981 agtgcgttcg ccgcgccgcg gtgggcccgg gacgtcgggc tctggagctg gatcgtcgtc
     2041 ggcgcgctcc tcgtgctgct cgcgctcttc tgggcggcgg cgtcgatcca gatggtcgtg
     2101 ctggcgtcgc tcttcgcggt gctcttcggg ggcacgttcc tgcccgtggt ggactggctc
     2161 cacaagcacc gtctgccgcg ctggctcggc gcgctgctcg tgctcgtctt cctcatcgcc
     2221 ctcggaatct tcatcgccct ggtcatcctc tacggcctgg tgaagcagct gcccgtgatc
     2281 caggagaacc tgcaggccgc gttcgactcc atcagcgagg cgctcgcgtc cacgagcgtc
     2341 gaccagtcga ccctggactc gatgaagacg gcgatcgggg gcgtgctcga gttcgcggcg
     2401 accggcgccg ccggcacggt cgccagcatc gccagcggcc tggcgtcgtt gatcttcggc
     2461 gtcttcatca gcatcaacat cctcgtgtgg gtgctgatcc aggggaggaa gatcgcctcc
     2521 tgggcctcgc ggcacgtccc gcctgtgccg cagtccgtgg cctacacgat cttcgccaac
     2581 agcgcacgct tcttccgcgg ctacatcttc gggagcacgc tcgtcggcct gttcaacgca
     2641 gcggtcatct tcgtgggggc gctcatcatc ggcgtgccga tgcccgggac cctggccatc
     2701 gtcgcctgga tgatgaacta catcccgttc ttcggcgcga tcgtcagcgg cgcgttcgcc
     2761 gtgctcatcg cctacggggc cggagggctg tcgatggcga tcccgatgct gatcatcgtc
     2821 atcatcgcca acggcttcct gcagacgctg gtgtcgcagt tcgccctggg ctctaccctc
     2881 gatctgcacc cgctggtggt actgttcgcg accaccgccg gcggcatcct gttcggcgcc
     2941 gtgggcggcg tgttcgcggc gccgttcgtg aagatcggcc tcgacgccta cgcgcgggtc
     3001 aaggccgcag gcctgttcgg cagtgggaag cagtccccca acgccgcggg tgcgtcgcat
     3061 tctccggccg cagaggcggt cgatctcgaa tcgctcgtcg tcgccgaagg caccggcagc
     3121 taggagggga ggaaccgtgg gcgtggagac gggcggagac gccttcgagg gagtgccggc
     3181 ggcgcggcgg ctggggcgga cgctgcgccg gcaccggcgc ctgcgcatta ccctcatcca
     3241 gctggcctac gtggccggcg ggatcgcctt ggggctcgcc ctgccgcgca tccccgtggg
     3301 gttcacggtg ccgcgcgccg agaccactca gatgctgttc gcggtgggcg ccggccttct
     3361 cggcttcctc gcgatcgcct tctcactggc gttcctcgtc gtgcagttcg gctcgaccac
     3421 gtacaccccg cgcctgaacc tcttctacac ctcgccgagg atctggcacg gtttcggctt
     3481 catcaccggc gtgctcgtct tcgccttcgc cgccgcctac agcgagacgt tcgcgtctgc
     3541 ctccggcggg gcgagcgacg agcgcatgag cggcctcgtc ccgatcgtcc tgatagccct
     3601 gctcatgggg gcgatcgtcg tgtaccgcaa cctgcagatg cgcgccttcg gctccgtcga
     3661 gctcggctcc gtactcgcgg aagtgaccga gcgagggcgc gaggtcctcg acggcatgta
     3721 cgcggacgag ccgccgcggg aagccgacgg accaccgcag caagacggag agcagcgcgg
     3781 tctccgcgcc cttcccgagg ggagccgcga ggtcgcatgg ccggggcgct ccggcatcat
     3841 ccaggacgtc gacgtcccgc gcatcatcgc ggcggcccgc agcgccgacg cggcggtcga
     3901 gatcgtcgtc cccaccggcg agatggtgca ccaccgggcg acggtcgccg tcatccacgg
     3961 ctccgtggac ccctcgctgg acgcggcggt ggtgaaggcc gtccggacgg gggcaggacg
     4021 caccttcgag caggacccga cgatggcgtt ccgcgtcctc gtcgacatcg ccctgcgggc
     4081 gctgtcaccg gcgatcaacg accccacgac tgccgtgcag gtcctggact gcgaggagga
     4141 cctgctccgg atgctcgcgg accgcgacct cgacgccggc gagatcaccg ggccccgcgg
     4201 caggacgcgc gtcctcctcc ccctgccgag ctgggacgac tacgtggccc tcgccgtcga
     4261 cgagatcgtc gaggcgggtg ccgctcaagt gcgcatccgc cggcgcgtcg aatgcctcct
     4321 gcgcgagctg acagctctgg cgcccgagag tcgccgagag ccactccagg cgcggctgga
     4381 cgagctggca tcgcaatggc agccggccgg ctcgggcaca gcaacgactc tgtcgggtga
     4441 gcacccggga gcgtgacaag acctcgtgag tcctcagaca ggaggagcgc cagcaccggc
     4501 ggactcaccg tcaggagctg cgcaaggaga gtcgatggcg acgtcacgca cgaagtggtg
     4561 gtcgcgcgcg cgagcggcag tcgccgctgc aggagggccg atcaaggagc acgatctccc
     4621 gcggcacgcc gcggcgctca cgtattacct cgtcttggcc ctcttcccga tcctcatcgt
     4681 ggtaacggcg ctcctgggag tgatcgggct cacgcccgag gcgctgcagt cgctgctcga
     4741 cgccgtcgcg cagcttggct cgccgtgggc cgtcgagttc gtgcgcggcg tactggacag
     4801 tgtccttgcg agcagcggaa cgccactgct gctcagcgtc ggcgtcctga tggcgctgtg
     4861 gactgcctcc ggctacgtgg cggcgttcat gtgggccgcg gccgggatcg gcggcgagcg
     4921 cgatacgcgg cgctggttca gcggcctcgc cgtgcggctc gggctcgcgc tgctgctcgc
     4981 cgtcctgctc tccctcgccg cgacggtggt tgtcctggcg acccccttcg cccagtggct
     5041 gggggacctg ctgggcatcg gagaaacggc ggtccaggtc tggacgacgg tcgactggcc
     5101 gttcttcttc ctggtcgccg tggccgtctt cctgctgctc taccgcgccg caccgcacag
     5161 ccacaggcgg cgcgtcctcc acgatctgat cggtgcctgc tgcggtgtga ccatctggct
     5221 catcgcctct gcggccttca gcttctacct tgcgaacttc ggctcgtatg accgcgtgta
     5281 cggcgtgctc ggcaccggca tcgccttcct ggtgtgggca tggatcctgg acctgactct
     5341 actcggaggg ctcgaagtga gcgtgggcct gggcacgtgg cgcgagcgtc ccaaggcgaa
     5401 gggcgacgcc gctgtgccac ccgagccgtc acctcttgag ccaagaacgc cccacgctgg
     5461 tggagcggga agatgagcgt tcgaatcgag gcgccccgcg atggcctctc agacgactga
     5521 gcattccgcc cacagcgtgc cgacatcctg tcgcgtgcgc ggtcaagccg ctggaggttg
     5581 agatgaccga gctttcatca tctgaggtca catctaggaa agtggcgcgc cgggccgcga
     5641 cgccgatcgc cggcgcgatc gccggcatca tcttcgcggt gctgtacacc gtgagcatga
     5701 ccgcccttcg tcagaccgtg ggcgacgccg ccagcgacac gggtgcctgg ctgaccgaag
     5761 acacgggact cgtcaagttc gccctcgccc tcgttccgtt cgctgggctc gcgttcctct
     5821 ggttcatcgc ggtggcgaga gagcgcttgg gcaggttcga ggatcagttc ttctcgacgg
     5881 tgttcatcgg cagcggtctg ctgttcctgg ccatggtctt cgtcgccgcg gcgaccgcgg
     5941 gcgcgatcgt cgccgcggcg gcgaacgacc ccgaagggtt cgcggccagc accacgtaca
     6001 cctacgcccg tcaggcggtc actcagatcc tgggcgtcta cgcggtgcgc atggcggcca
     6061 tcttccagct ctcgcaggcg agcctgtggc tgcgcaccca cgtgatgccg cgctggatgg
     6121 cgctgatcac catcgtggtc gcgctggtcc tgctcttcgt cttcacccag tcctggtggg
     6181 tcgtcctcgt cttcccggcc tgggtgctgc tggtcagcat ctacattctg atcgcgcagc
     6241 cggcgcagga agagagggcg gctggggagc gccggccgta gccgaggctc cgtccgcgac
     6301 taccgggcgg tcgctcgtat cgcgaactgc cctcagccca gcgcgatgcc ctcggacagg
     6361 aactgcttct gcagctcgcg gagataggcg tcctgggcga ggcgccgccg ccggtagtgc
     6421 ttgatgccga ggcgggtcac gcacgtggcg ccggcccccg cgagcgagac cacgtcgcac
     6481 gtcgggggga agctctcctc gacctctgcc gacgccccgg cggtggcgcg ggcggcctcg
     6541 accgcgacgt gcacgacgcg gtcgagatcg gcgtcggcga cgaccttgaa ctcgacggc
//
LOCUS       DASUMX010000031         4545 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_584497, whole genome shotgun sequence.
ACCESSION   DASUMX010000031 DASUMX010000000
VERSION     DASUMX010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4545
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584497"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..1318)
                     /locus_tag="VFZ86_01360"
     CDS             complement(110..1318)
                     /locus_tag="VFZ86_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5640965.1"
                     /translation="MSRVLRGLVLLTFVLALAAGLAAVAQARAVATASKPAFSQAPVA
                     GVPFTASGVVRPRATAVSQTVVKVRLYTRAGGHWRVKGTYRAKLAAGTSGTKYARRLT
                     VAAEGRYAVRAFHYRAGKLVATSALASFQVARRVTIDANVNGWLAPGLRDTTAPAGTP
                     LDIVFTKPADWATPDPAKLNGAAHFVWGGFEKVDADGLIWHTDGLRPGFYDWMRDGMP
                     KWGTGSLVVTQRIDVDKNAHGDTHALPYVPADISFGDVSSAGMGCDRSIAFLTRIFTQ
                     ASPDPLMWHSEGLVPGRYDWKCWMPDCHYGTLVADGAAQAVAIDTDPDNSVTAVPSGT
                     ALDLVFSSFSGTGMMCWRTIFFTTAGELTKTRAYPDPLTWYSGGLAPGNYEWECWMGP
                     GCHHGTIAVQ"
     gene            complement(1679..2980)
                     /locus_tag="VFZ86_01365"
     CDS             complement(1679..2980)
                     /locus_tag="VFZ86_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HEX5640966.1"
                     /translation="MAIWDPQHETIDRERLEELQLARLRDMLAKVHERVPFSRRRLDE
                     AGFEPGDLKSLGDLAGLPFTEKTDFRDNYPYGLFAVPLSEVVEIHSSSGTTGKAVVGG
                     YTRADLETWAELVCRLAVAVGANEGDVAQIAFGYGMFTGGFGLHYGLQKAGVGVLPIS
                     AGNTARQIQFMKDFGTTILIATPSYALYIGEVLQRRGVPLEQLRLRLGMFGAETCSEG
                     MRAQIESRLPIRATDNYGLTELTGPGVAGECECRSGMHVAEDHFIAECLDPATGEPVA
                     DGEVGELVVTAMTRECSPVLRYRTRDLTSLTRERCACGRTTARMGKVVGRTDDMFIIS
                     GVNIFPSAIESVLFQIEGVEPFYQIVLGRSNGLDTFEVHVEVTDTLFDGWMDDLRAFE
                     RRVTEELRSALLVRPRVKLVEPGKLERSEGKARRVIDNRPD"
     gene            3169..>4545
                     /locus_tag="VFZ86_01370"
     CDS             3169..>4545
                     /locus_tag="VFZ86_01370"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX5640967.1"
                     /translation="MSDHETTIELPVTGMTCASCVARVERAIGKADGVDKASVNLATE
                     KATVTFDPGEVSTGDLVGVIEGAGYGVVTAQETLPIIGMTCASCVSRVEKALRNPPGV
                     LKADVNLATEKATVSYIPGQASYRDLVEAVRGAGYDVVEPAPGTGAEGIEAAADAEEA
                     ARAAAYRKLKLKVTVGFVLSIIIFAGTMQMEWFTFLPAWMHNGYFLWALASVVQFWVG
                     WQFYTTAWSALKHGTTTMNTLVALGSSAAYLYSVLGVLFPAFFEHQGLGEPMYFDSSA
                     FIITLILLGRLLEARAKGQTGAAIKALIGLQPRTARVLRDGVETDVPIAEVVVDDAVL
                     VRPGEKVPVDGVVTSGSSPVDESMLTGEPLPVTKRADDEVIGATMNTTGSFTFRATKV
                     GADTALAQIIRLVEQAQGSKPPIARLADVIAAWFVPAVIVIATVTLIVWLILGPSPAL
                     NYALLNFVA"
BASE COUNT          667 a         1602 c         1596 g          680 t
ORIGIN      
        1 cgtgaagtag gtgatgcggg cgctggccac cgacgccgtg cacgccagca gcgtcaccac
       61 caggcccacc atcaggatgg ctctcagata cgtgcgcagg atgtccctcc tactggacgg
      121 cgatggtccc gtggtggcag cccggtccca tccagcactc ccactcgtag ttgccggggg
      181 cgagcccgcc ggagtaccag gtcagcgggt cggggtaggc cctggtcttg gtgagctcgc
      241 ccgccgtcgt gaagaagatg gtccgccagc acatcatccc ggtaccgctg aacgagctga
      301 acaccaggtc cagcgccgta ccggacggga ctgccgtcac ggagttgtcg gggtcggtgt
      361 cgatggccac cgcctgggcg gctccgtcgg ccaccagcgt cccgtagtgg cagtcaggca
      421 tccagcactt ccagtcgtag cgcccgggga ccaggccctc gctgtgccac atgagggggt
      481 cggggctcgc ctgcgtgaag atgcgcgtga ggaaggcgat gctgcggtcg cagcccatgc
      541 cggccgagct gacgtcgccg aaggagatgt ccgccggcac gtaggggagc gcgtgcgtgt
      601 cgccgtgtgc attcttgtcg acgtcgatcc gctgcgtgac gaccagggag ccggtgcccc
      661 acttgggcat cccgtcgcgc atccagtcgt agaagccggg gcgcaggccg tcggtgtgcc
      721 agatgagccc gtcggcgtcg accttctcga agccgcccca gacgaaatgc gcggcgccgt
      781 tcagcttcgc cggatcgggt gtggcccagt cggcgggctt ggtgaagacg atgtcgagcg
      841 gcgttccggc gggagccgtc gtgtctctga gccccggcgc cagccagccg ttgacgtttg
      901 cgtcgatggt gacccgccgg gccacctgga acgaggcgag cgcggacgtc gcgacgagct
      961 tgcccgcgcg gtagtggaac gccctgacgg cgtacctgcc ctccgcggcg acggtgagcc
     1021 gccgggcgta cttcgtgccg gaggtgccgg cggccagctt ggcgcggtag gtccccttca
     1081 ccctccagtg gccgcccgcg cgcgtgtaca gcctgacctt gacgacggtc tgggacaccg
     1141 ccgtcgccct gggccggacg acgcccgagg cggtgaacgg cacgccggcg accggcgcct
     1201 gcgagaacgc gggcttgctc gccgtggcga ccgctcgcgc ctgggcgacg gccgccaggc
     1261 ccgcggccag cgcgaggacg aacgtcagca ggaccagccc tctcagaaca cgtgacatgt
     1321 gacccctccc ccatcgtggc cgccccagcg gcgaagacca gatcttcgcg cttcatggta
     1381 aacgaggcac aggccggtcg ctacaggcgc cgttgcccgc gccgaggctc agcgacaacc
     1441 ggcgtcgtct gcggtgacaa cacgcgcgcg agcctcctag acgcgtgttc tatgcgcccg
     1501 cggacggcgg cgcccgatgt catggccagg aggccggaag actctgagag aggaacttcc
     1561 aagtcgacct tgaccttggc cggcggcgca aagcgggggc ttgcgccgtc ggcctgcacg
     1621 tacgtgcgga ccggcggcgc acccgccgcg gccgtggccg gcgccgccgg cgcgaggctc
     1681 agtccggccg gttgtcgatg acgcgccgag ccttgccctc gctgcgctcc agcttgcccg
     1741 gctcgaccag cttgaccctg ggccggacga ggagcgccga gcgcagctct tcggtgaccc
     1801 ggcgctcgaa cgcgcgcagg tcgtccatcc agccgtcgaa cagggtgtcc gtgacttcca
     1861 cgtggacctc gaacgtgtcc agcccgttcg agcgtcccag cacgatctgg tagaagggct
     1921 cgacgccctc gatctggaag agcacgctct cgatcgccga ggggaagatg ttgacgccgc
     1981 tgatgatgaa catgtcgtcg gtgcggccca cgaccttgcc catgcgcgcc gtggtgcggc
     2041 cgcaggcgca gcgctcgcgc gtgagcgacg tcaggtcgcg cgtgcggtag cgcagcaccg
     2101 gcgagcactc ccgcgtcatg gcggtgacca cgagctcgcc gacctctccg tcggcgaccg
     2161 gctcgccggt cgcggggtcg aggcactcgg cgatgaagtg gtcctcggcg acgtgcatgc
     2221 cgctgcggca ctcacactcg ccggcgacgc cgggcccggt gagctccgtg agcccgtagt
     2281 tgtcggtcgc cctgatcggc agccggctct cgatctgggc gcgcatcccc tcgctgcagg
     2341 tctccgcgcc gaacatgccc agccgcaggc gcagctgctc gagcggcacg ccgcgccgct
     2401 gcagcacctc gccgatgtag agcgcgtagg acggcgtggc gatgaggatc gtggtcccga
     2461 agtccttcat gaactggatc tggcgggcgg tgttgccggc gctgatgggc agcacgccga
     2521 cgccggcctt ctgcaggccg tagtgcaagc cgaacccgcc ggtgaacatg ccgtacccga
     2581 aggcgatctg cgccacgtcg ccctcgttgg cgccgacggc gacggcgaga cggcagacga
     2641 gctcggccca ggtctcgagg tcggctctcg tgtagccgcc gaccaccgcc ttgcccgtgg
     2701 tgcccgacga ggagtggatc tcgacgacct cgctcagcgg gaccgcgaac aggccgtagg
     2761 gatagttgtc gcgaaagtcg gtcttctcgg tgaagggcag cccggcgagg tcgcccaggc
     2821 tcttgaggtc gccgggctcg aacccggcct cgtccagccg gcgccgggag aacgggacgc
     2881 gctcgtgcac cttggcgagc atgtcgcgca gacgggcgag ctgcagctcc tcgagccgct
     2941 cgcggtcgat ggtctcgtgc tgcggatccc agatggccat ggtgtctcgc ttcctcgctg
     3001 ccggtgccgc gggcaatcat accggggcgc ggaccgtcgg ccgcagcata ccccccgcgg
     3061 gtaccatcgg ccgcgtccgg gaacgtatcg tttgagcccg ggaacactta ccggggcgag
     3121 tggacctgcc gctcgtcgcg tccactggaa caccggtcgg gagcacgtgt gagcgaccac
     3181 gagaccacca tcgagttgcc ggtcaccggc atgacctgcg cgagctgcgt cgcgagagtg
     3241 gagcgcgcca tcggaaaggc ggacggcgtc gacaaggcgt ccgtcaacct cgccaccgag
     3301 aaggcgaccg tgacgttcga cccgggcgag gtctcgaccg gcgacctggt cggggtcatc
     3361 gagggcgccg gctacggcgt cgtgaccgcg caggagacgc tgccgatcat cggcatgacc
     3421 tgcgcgagct gcgtgagccg ggtggagaag gccctgcgca acccgcccgg cgtcctcaag
     3481 gcggacgtca acctggccac ggagaaggcg accgtgtcgt acatcccggg ccaggcgagc
     3541 taccgggacc tggtcgaggc cgtgcgcggc gccggctacg acgtcgtgga gccggcgccg
     3601 ggcacgggtg cagagggcat cgaagcggcg gcggacgccg aggaggcggc ccgcgccgcc
     3661 gcctaccgca agctcaagct caaggtcacc gtcggcttcg tgctcagcat catcatcttc
     3721 gccggcacca tgcagatgga gtggttcacg ttcctgccgg cgtggatgca caacggctac
     3781 ttcctctggg cgctcgccag cgtcgtgcag ttctgggtgg gctggcagtt ctacaccacc
     3841 gcctggtcgg cgctgaagca cggcaccacg accatgaaca cgctcgtcgc cctgggctcc
     3901 tcggcggcgt acctctacag cgtcctcggc gtgctcttcc cggcgttctt cgagcaccag
     3961 ggcctgggcg agcccatgta cttcgactcc tcggccttca tcatcacgct catcctcctc
     4021 gggcggctgc tcgaggcgcg cgccaagggg cagacgggcg cggccatcaa ggcgctcatc
     4081 ggcctgcagc cgcgcacggc gcgggtgctg cgcgacggcg tcgagaccga cgtgcccatc
     4141 gcagaggtcg tcgtggacga cgccgtgctg gtgcgtcccg gcgagaaggt gccggtggac
     4201 ggcgtcgtca cgtccggctc ctcgccggtg gacgagtcca tgctgaccgg cgagccgctc
     4261 ccggtcacca agcgcgccga cgacgaggtc atcggcgcca ccatgaacac caccggctcc
     4321 ttcaccttcc gcgccaccaa ggtcggcgcc gacaccgccc tcgcgcagat catccgcctc
     4381 gtcgagcagg cacagggctc caagccgccc atcgcccggc tggccgacgt gatcgccgcc
     4441 tggttcgtgc cggccgtgat cgtcatcgcg accgtcacgc tgatcgtctg gctcatcctc
     4501 ggcccgtctc cggcgctcaa ctacgcgctg ctcaacttcg tcgcg
//
LOCUS       DASUMX010000032         8524 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_440614, whole genome shotgun sequence.
ACCESSION   DASUMX010000032 DASUMX010000000
VERSION     DASUMX010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 8524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8524
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440614"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..258)
                     /locus_tag="VFZ86_01375"
     CDS             complement(31..258)
                     /locus_tag="VFZ86_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HEX5640968.1"
                     /translation="MKEQVEKALGDIRPALQADGGDIELVDVLDDGVVQVRLQGACAG
                     CPMSQLTLTNGVERHLKNVVPGVERVENMGF"
     gene            complement(276..722)
                     /locus_tag="VFZ86_01380"
     CDS             complement(276..722)
                     /locus_tag="VFZ86_01380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX5640969.1"
                     /translation="MKLSTQADYAVRAVYELARHDPGVVLHTGDIAAAQRIPGPRLAK
                     VIHDLARADLVRTQRGHQGGVMLARPAADITVRDVYEAVEGPILLCRCRQRVEPCGDD
                     VCDTHDFWHGVETLLTEELQTMTFAALTDARRRACVSSGRPQATGR"
     gene            complement(802..1596)
                     /locus_tag="VFZ86_01385"
     CDS             complement(802..1596)
                     /locus_tag="VFZ86_01385"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5640970.1"
                     /translation="MKQLAPDVWAYTIDAEGFPTTSAVVLTPRVAIVVDTLTGPAEVA
                     PVAEFVASEAGVRRVVVVNTHHHWDHVFGNAAFAGEDIVAQRGCPRLIQAELQGGDES
                     LRLPPSEGVPLPNVTFGDRLTYSDDGGAVHLIHTPGHSEDSIVVFLSGSRLLLGGDTV
                     EWPLPNFGQRDGRDDWVRTLRRLKQLPADLVVPAHGPAMGKQIIDANELYVTGVYEAV
                     AEAKAAGAARGELDLPAGRFLAAGVAVDDVYAAAHRDNLLWAWDEV"
     gene            complement(1691..2914)
                     /locus_tag="VFZ86_01390"
     CDS             complement(1691..2914)
                     /locus_tag="VFZ86_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEX5640971.1"
                     /translation="MTADGPQLVQPGAALVPAAELTARIAGLQATLAAQELDAALLQQ
                     NADLYYFAGTVQQSMLYVPAAGEATLFVRKVAERARMESALGGIVALASPKDLPALLA
                     ARYGRLPARLGLELDVLPVTHFRRLEKLLPEAAFEDVGRAIVRQRAVKSAWEIERIAA
                     AAAVADEVAGLIPGLLTDGLTEVEFAGRVEAEARRLGHEGYIRMRGFNQEMFYGQLLT
                     GVSGCVPSYLDTPLAGTGLSPSVAQGAGFRRIGRGEPVVFDFVPVRDGYMADFTRMYA
                     LGPVDDELLRVHDAALRVQDAATAAARPGVPCRAVWEAAKGAAADAGLAGGFMGRGAG
                     QVRYVGHGIGVELDELPVLTGSDLELEAGMVFALEPKFVLPSVGAVGIENTWAVTGDG
                     VERLTHAPDVFTVVE"
     gene            complement(2916..3893)
                     /locus_tag="VFZ86_01395"
     CDS             complement(2916..3893)
                     /locus_tag="VFZ86_01395"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX5640972.1"
                     /translation="MGDVYRFPGGDMPPDFETKLPQDLPVRYCYRHQDRETGVSCSNC
                     GRPICHDCMIPAPVGFRCPECVKEQSVRGSRAKVVTRGQIRSGWSAGAMGGAAGLSAT
                     KVLIGLNLVMFVIELLVGATGIMGGGSAQAMLNLGGLWPRAVLQEHEYWRMITSMFLQ
                     DGIFHILFNMWALWAIGGFMEAALGRVKFVLLYFISGFAGSVLVLVAAPTDSLVVGAS
                     GAIFGIFGALAVHAFLNRGRDFQSRALLGNVIFLLVINLVFTFSAGRVSWQAHIGGLL
                     AGAAVMAAMMLGGRKDPRRPFGMADGLAVAGIVFFLLLVTAWQVVTFVA"
     gene            complement(3896..4336)
                     /locus_tag="VFZ86_01400"
     CDS             complement(3896..4336)
                     /locus_tag="VFZ86_01400"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019987417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX5640973.1"
                     /translation="MSTSPSPTSSFETRVRVRYADTDADGVVYYANYLAFFEVARVEW
                     LRALGYPITRILAEGVILPVVEARVRYLRPARVDDLLTVRATLASVGPASFAFDYEVA
                     REDGLLLVTGSTRLAVCERETSRACAMPAWLRDLFADLPVTVQD"
     gene            complement(4416..5690)
                     /locus_tag="VFZ86_01405"
     CDS             complement(4416..5690)
                     /locus_tag="VFZ86_01405"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5640974.1"
                     /translation="MPDKPTYMQHMPHFLGPVAPITIDHAEGSWVFDTDGGKWLDFVV
                     GIAVVNTGHCHPKVVKAVQEQAAKVPHAQMNIYRHGPMLELAEKLTGIMPEGMDEVMF
                     ANSGAEAVENAVKLAKQVTRRPGIIAFQGAFHGRTHLTMALTDSAVHYRGHHEPLVGS
                     IYHARYCYPFRTPASEDPTAYAIEDVRRVLRAEIYGDDVAAIIVEPIQGEGGFIVPTA
                     EFMQELRAIADEIGACLIIDEIQAGFGRTGKWLCQEHHDVKADIITVAKGIASGYPLG
                     AIAANHDLWAKCLAGSMGGTYGGNAVACAAGVATMDAIVEDGMLANATKQGEKLLKFW
                     KEKEAQYAVIGEARGKGLMLAIECVQPGTKEPNAAAAKAFIAECQKHKVILMGACSQG
                     NGVRFLPPLNISDADMDLAIGIFDEAAKVAFA"
     gene            6006..7046
                     /locus_tag="VFZ86_01410"
     CDS             6006..7046
                     /locus_tag="VFZ86_01410"
                     /inference="COORDINATES: protein motif:HMM:NF003995.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEX5640975.1"
                     /translation="MVSRKTVSRMSRYRRLLQSLRETGVESIYSHQLARHAVVSAAQV
                     RRDLMVIGYSGSPNKGYDVTACIDSISHFLDGPVRQEVALVGAGNLGHAVLSHFADKS
                     PTVAIVAAFDVDPALTGTLIHGVRCLDARLMESLVRDLRLQIAVLTVPGTVAQEVAET
                     LVRAGVKSIISFAPTPLALPNDIFVEYMDITAALESAAFFARLGGRDEPPANGDGDIE
                     PIVRKLESLLARSNMKLEELAARIGADVVTPGKAGGTSVARIYAGDRVSDLLNEASDK
                     TLLVSNLASVQMLRVAELMDVPGICFVNGIRPDEEMVQLARDNDTLLMVSPQGVFETC
                     GRIYQTLDGEHA"
     gene            7050..>8524
                     /locus_tag="VFZ86_01415"
     CDS             7050..>8524
                     /locus_tag="VFZ86_01415"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HEX5640976.1"
                     /translation="MEHLAYDIRCGDFTSAGAASRDLKQHLKRIGAESDAVRRAMIAA
                     YEAEMNVVIHAEGDGHLEAAVSDGQLDVDVVDHGPGIPDVESAMREGWSTASSEARAL
                     GFGAGMGLPNILRNSDRLSVTSVAGEGTRVSFSVALRPEATDQGARPSSLGVVAELCK
                     ECRHCLVACPTAAMRVREARPDVLYHLCIDCTACIGACAPRALTMSDAPGALTGGDVL
                     VVPPALLAGFGEHPVSAVVEELRGLGYDEVVSVHHYEDALRRAVIELTATGDAPAPLI
                     SPVCPAVVNLVEVKFPSLLEHLVPLASPWEAARQDLDGRAATFAVSCPSQRTALLAQR
                     PTAQRDAVTAAAVREAVLPRLAARARRTPGATASPPAAGGADDLLVVTGVAHVLAVLE
                     AVEDDRLPGVSAIEPYICDGGCFGSPLLGEDASVAAWRWSAVGGDAPGDGGTHERSRP
                     FRARPGIRLDADMAAAIRKLAQLDAETRALPGRDCGVCGAPT"
BASE COUNT         1237 a         3057 c         3027 g         1203 t
ORIGIN      
        1 ccgccgcccg ccgctcgcgt cttcgtcgtc tcagaagccc atgttctcga cccgctccac
       61 gccgggcacc acgttcttga ggtggcgctc cacgccgttg gtcagggtga gctgggacat
      121 cgggcacccc gcacaggctc cctggaggcg tacctggacg acgccgtcgt ccagcacgtc
      181 gaccagctcg atgtcgccac cgtcggcctg cagagccggg cggatgtcgc ccagggcctt
      241 ctcgacctgc tccttgatct ctgccatatg aattgtcacc tccccgtcgc ctgcgggcgg
      301 ccactggaga cgcaggcgcg tctccgcgcg tcggtcaggg ccgcgaacgt catcgtctgg
      361 agctcctccg tgaggagcgt ctcgacacca tgccagaagt cgtgggtgtc gcacacgtcg
      421 tcgccgcagg gttctacccg ctgacgacac cggcaaagca ggatggggcc ctccaccgcc
      481 tcgtacacgt cgcgcacggt gatgtcggcc gccgggcgcg ccagcatgac cccgccctgg
      541 tggccgcgct gggtgcgcac caggtcggca cgcgccaggt cgtggatgac cttggcgagc
      601 cgcggccccg ggatgcgctg cgccgcggcg atgtcgccgg tgtgcagcac cacgccgggg
      661 tcgtgccgcg ccagttcgta gacggcgcgc accgcgtaat cggcctgtgt ggagagcttc
      721 atgatgtctt tttacaccaa ttagaagaca atgtccaatt cctcgccccg ccgagcgccc
      781 ggacgtgccg gcggcggccc ctcagacctc gtcccaggcc cagagcaggt tgtcgcggtg
      841 ggcggcggcg tacacgtcgt cgacggcgac gccggcggcg aggaagcgcc ccgcgggcag
      901 gtccagctcg ccgcgcgcgg cgcccgccgc cttggcctcg gccacggcct cgtacacgcc
      961 cgtgacgtag agctcgttgg cgtcgatgat ctgcttgccc atcgccggcc cgtgcgccgg
     1021 gacgaccagg tcggccggca actgcttgag ccggcgcagc gtgcgcaccc agtcgtcgcg
     1081 gccgtcgcgc tggccgaagt tgggcagcgg ccactccacc gtgtcgccgc cgagcagcag
     1141 ccggctgccg ctgaggaaca ccacgatgga gtcctcgctg tggccgggcg tgtggatcag
     1201 gtggaccgcc cccccgtcgt cgctgtaggt gagccggtcc ccgaaggtga cgttgggcag
     1261 cgggacgccc tcggacggcg gcagccgcag cgactcgtcg cctccctgca gctcagcctg
     1321 gatgaggcgc ggacaccccc gctgcgcgac gatgtcctcg ccggcgaagg cggcgttgcc
     1381 gaacacgtgg tcccagtggt ggtgcgtgtt cacgacgacc acccgccgca ccccggcctc
     1441 gctcgcgacg aactcggcca caggagcgac ctcggccgga cctgtcagcg tgtccacgac
     1501 gatggcgacg cgcggcgtga gcaccaccgc cgacgtcgtc gggaacccct cggcgtcgat
     1561 ggtgtacgcc cagacgtccg gcgccagctg cttcatcgcc tgtcctccat gctcgggtcc
     1621 ggatgtgcta cgcccactct tccccaccgc gcggagtcca gccgccgggc gcccacgccc
     1681 ggcagcggct tcactccacg acggtgaaga cgtccggcgc gtgcgtgagc cgctccacgc
     1741 cgtcgccggt gaccgcccag gtgttctcga taccgacggc gcccacgctc ggcaggacga
     1801 acttgggctc gagcgcgaag accatgccgg cctcgagctc gaggtcgctg ccggtgagca
     1861 ccggcagctc gtcgagctcg acgccgatgc cgtggcccac gtaccgcacc tggccggcgc
     1921 cgcgtcccat gaagcctccg gccaggccgg cgtccgcggc ggctcccttc gccgcctccc
     1981 agacggcacg gcacggcacg ccggggcggg ccgcggccgt cgccgcatcc tgcacgcgca
     2041 gggcggcgtc gtggacccgg agcagctcgt cgtccaccgg ccccagcgcg tacatgcgcg
     2101 tgaagtcggc catgtagccg tcgcgcaccg gcacgaagtc gaacacgacc ggctcgccgc
     2161 ggccgatgcg gcggaagccg gcgccctggg cgaccgacgg gctgaggccg gtgccggcga
     2221 gtggcgtgtc caggtagctg ggcacgcagc cgctgacgcc ggtgagcagc tgcccgtaga
     2281 acatctcctg gttgaacccg cgcatgcgga tgtagccctc gtgccccaga cgccgcgcct
     2341 ccgcctccac gcgaccggcg aactcgacct cggtgagacc atccgtgagg aggccgggga
     2401 tcagtccggc gacctcgtcg gcgacggcgg cggcggccgc gatgcgctcg atctcccacg
     2461 ccgacttgac cgcccgctgc cgcacgatgg cccggccgac gtcctcgaac gccgcctcgg
     2521 gcaagagctt ctcgaggcgg cggaagtgcg tcaccggcag cacgtccagc tcgaggccga
     2581 gacgcgcggg gaggcggccg tagcgcgcgg ccagcagcgc cggcaggtcc ttgggcgagg
     2641 cgagcgcgac gatcccgccg agcgccgact ccatgcgggc gcgctcggcg accttgcgca
     2701 cgaacagcgt cgcctcaccc gcggccggca cgtacagcat cgactgctgg acggtgccgg
     2761 cgaagtagta caggtcggcg ttctgctgca gcagcgcggc gtcgagctcc tgggcggcaa
     2821 gggtggcctg caggccggcg atgcgggccg tcagctccgc cgccggcacg agcgccgcgc
     2881 ccggctgcac cagttgcggc ccgtccgcgg tcacgtcagg cgacgaaggt cacgacctgc
     2941 caggcggtga cgaggaggag gaagaagacg atgccggcga cggcgagccc gtcggccatg
     3001 ccgaacggcc ggcgcgggtc cttgcggccg cccagcatca tcgcggccat caccgccgcc
     3061 cccgccagca gcccgccgat gtgcgcctgc cacgacaccc ggccggcgga gaacgtgaac
     3121 accaggttga tcacgagcag gaagatgacg ttgccgagca gcgcccgcga ctggaagtcg
     3181 cggccgcggt tgaggaacgc gtgcacggcg agggcgccga agatgccgaa gatcgccccg
     3241 gaggcgccca ccacgagcga gtccgtgggc gccgcgacca ggaccagcac cgatccggcg
     3301 aaccccgaga tgaagtagag cagcacgaac ttgacccgcc ccagcgccgc ctccatgaag
     3361 ccgccgatgg cccacagcgc ccacatgttg aacaggatgt ggaagatgcc gtcctggagg
     3421 aacatggagg tgatcatgcg ccagtactcg tgctcctgga gcacggcgcg cggccagagg
     3481 ccgccgaggt tgagcatcgc ctgcgccgac cctccgccca tgatgcccgt cgccccgacg
     3541 agcagctcga tcacgaacat gacgaggttg aggccgatga ggaccttggt cgcggagaga
     3601 cccgcggcgc cgcccatcgc cccggcgctc caccccgagc ggatctggcc gcgggtgacg
     3661 accttggcgc gcgagccgcg gacgctctgc tccttgacgc actcggggca gcggaagccg
     3721 acgggcgccg ggatcatgca gtcgtggcag atcggacgcc cgcagttgga gcacgagacc
     3781 ccggtctcgc ggtcctggtg acgatagcag tagcggaccg ggaggtcctg cggcagcttc
     3841 gtctcgaaat ccggcggcat gtcgccgccg gggaacctgt acacgtcgcc catggtcagt
     3901 cctgtaccgt gacgggcagg tcggcaaaca ggtcgcgcag ccaggcgggc atggcgcagg
     3961 cgcgcgaggt ctcgcgctcg cacacggcga ggcgggtcga gccggtgacc agcagcagcc
     4021 cgtcctcgcg cgcgacctcg tagtcgaagg cgaacgacgc cgggccgacg gaggcgaggg
     4081 tcgcgcggac cgtgagcagg tcgtcgacgc gcgccgggcg caggtagcgc acgcgcgcct
     4141 cgacgaccgg caggatgacg ccctcggcga ggatgcgcgt gatggggtag ccgagggctc
     4201 gcagccactc gacgcgcgcg acctcgaaga aggcgaggta gttggcgtag tagacgacgc
     4261 cgtcggcgtc ggtgtccgcg tagcgcacgc gcacgcgcgt ctcgaagctg gaggttggcg
     4321 acggggacgt gctcacgagc ctgagtctag catgacggcg agggcggggc gccgcggcgc
     4381 cccgccctcg cggacgttcg ggtgtcgcgc gccggtcagg cgaaggcgac cttggcggcc
     4441 tcgtcgaaga tgccgatcgc gaggtccatg tcggcgtcgc tgatgttgag cggcggcagg
     4501 aagcggacgc cgttgccctg cgagcaggcg cccatgagga tgaccttgtg cttctggcac
     4561 tcggcgatga atgccttggc ggcggcggcg ttgggctcct tggtgcccgg ctgcacgcac
     4621 tcgatggcga gcatgaggcc cttgccgcga gcctcgccga tgaccgcgta ctgcgcttcc
     4681 ttctccttcc agaacttgag cagcttctcg ccctgcttgg tggcgttggc gagcatgccg
     4741 tcctccacga tggcgtccat ggtggcgaca ccggcggcgc aggccacggc gttgccgcca
     4801 taggtgccgc ccattgagcc ggccaggcac ttggcccaca ggtcgtggtt ggcggcgatg
     4861 gcgccgagcg gatagccgga ggcgatgccc ttggcgacgg tgatgatgtc ggccttgacg
     4921 tcgtggtgct cctggcacag ccacttgccg gtacggccga agccggcctg gatctcgtcg
     4981 atgatcaggc aggcgccgat ctcgtcggcg atggcgcgca gctcctgcat gaactcggca
     5041 gtaggcacga tgaagccacc ctcgccctgg atgggctcga cgatgatggc ggcgacgtcg
     5101 tcgccataga tctcggcgcg cagtacgcgg cgcacgtcct cgatcgcgta ggcggtcggg
     5161 tcctcgctgg ccggcgtgcg gaacgggtag cagtagcgcg cgtggtagat ggagcccacg
     5221 agcggctcgt ggtgaccgcg gtagtgcaca gcggagtcgg tgagcgccat ggtgaggtgg
     5281 gtgcggccgt ggaaggcgcc ctggaaggcg atgatgccgg ggcgacgcgt gacctgcttg
     5341 gcgagcttga cggcgttctc gacggcctcg gcgccggagt tggcgaacat gacctcgtcc
     5401 atgccctcgg gcatgatgcc ggtgagcttc tcggccagtt cgagcatcgg gccgtgacgg
     5461 tagatgttca tctgcgcgtg cggcaccttg gcggcctgct cctgcacggc cttgacgacc
     5521 ttcgggtggc agtggcctgt gttgaccacg gcgataccga ccacgaagtc cagccacttg
     5581 ccgccgtcgg tgtcgaagac ccagctgccc tcggcgtggt cgatggtgat cggcgccacc
     5641 ggcccgagga agtggggcat gtgctgcatg tacgtgggtt tgtccggcat gtggtgacct
     5701 ccagctgcga cggttctgtg cgtggtctgt gcgtgaactg acgagtccgg cggcgcgccg
     5761 gtcccccacg gctcagtgcc cggactctac cagagccact tcgtcccaga tgtgccaggt
     5821 caacgtacgc acgacacgac agacggggtc ttattggcca acttggacaa tgcgcggcgg
     5881 tgttcggttc tgcgcgagag tgttgacagg aggatgagca agagagagaa tcgtgacaga
     5941 attcacagcg ttcgttctgt aacgaacaag taacgagacc acacccaccg cccgggagcc
     6001 gtttcgtggt atcgaggaag accgtgtcac gcatgagccg ctaccggcgg ctgctgcagt
     6061 ccctgcgcga gaccggtgtg gagagcatct actcccacca gctcgcgcgt cacgccgtcg
     6121 tcagcgccgc ccaggtgcgc cgcgacctca tggtcatcgg ctactccggc agccccaaca
     6181 agggctacga cgtgaccgcc tgcatcgaca gcatctcgca cttcctggac ggcccggtgc
     6241 gccaggaggt cgcgctcgtc ggcgccggca acctcggtca cgccgtcctc tcgcacttcg
     6301 ccgacaagag ccccaccgtg gcgatcgtgg cggcgttcga cgtcgacccc gcgctcacgg
     6361 gcacgctcat ccacggggtg cgctgcctcg acgctcgcct catggagagc ctggtgcgcg
     6421 acctgcgcct gcagatcgcc gtgctgacgg tgccggggac ggtcgcgcag gaggtggccg
     6481 agacgctcgt gcgcgccggc gtgaagagca tcatcagctt cgcgccgacg ccgctcgccc
     6541 tccccaacga catcttcgtc gagtacatgg acatcacggc ggcgctcgag tccgccgcct
     6601 tcttcgcccg cctcggtggg cgcgacgaac cgcccgccaa cggcgacggc gacatcgagc
     6661 cgatcgtcag gaaactggaa tccctgctcg cgaggtccaa catgaagctc gaagaacttg
     6721 ccgcccgcat cggcgccgac gtggtgacgc cgggcaaagc gggcggtacc agcgtcgcca
     6781 ggatctacgc cggcgaccgc gtcagcgacc tgctcaacga ggcgtcggac aagacgctcc
     6841 tggtcagcaa cctggcgagc gtgcagatgc tgcgtgtcgc cgagctcatg gacgtgcccg
     6901 gcatctgctt cgtcaacggc atccggccgg acgaggagat ggtccagctc gcccgcgaca
     6961 acgacaccct gctcatggtc tcgccgcagg gcgtgttcga gacctgcggg cgcatctacc
     7021 agaccttgga cggagagcac gcctgagcca tggagcacct ggcctacgac atccgctgcg
     7081 gggacttcac gagcgccggc gcggcgtcgc gcgacctcaa gcagcacctc aagcggatcg
     7141 gcgccgagag cgacgccgtg cgccgggcga tgatcgccgc ctacgaggcc gagatgaacg
     7201 tcgtgatcca cgccgagggc gacggccacc tcgaggccgc cgtgagcgac gggcagctcg
     7261 acgtggacgt cgtcgaccac ggcccgggca tccccgacgt cgagtcggcg atgcgcgagg
     7321 gctggtccac ggcgtcctcc gaagcccgcg cgctggggtt cggcgccggc atgggcctgc
     7381 cgaacatcct gcgcaacagc gaccgcctga gcgtcacgtc cgtggccggc gagggcacgc
     7441 gcgtcagctt ctccgtggcg ctgcggccgg aggcgaccga ccagggggcg cggccctcct
     7501 cgctcggcgt cgtcgccgag ctctgcaagg agtgccgcca ctgcctggtc gcctgcccga
     7561 cggccgcaat gcgcgtgcgc gaggcgcgcc cggacgtgct ctaccacctc tgcatcgact
     7621 gcaccgcctg catcggcgcc tgtgccccgc gcgcgctgac gatgagcgac gcgcccggcg
     7681 cgctcaccgg cggcgacgtg ctggtcgtgc cgccggcgct cctcgccggg ttcggcgagc
     7741 accccgtgag cgccgtcgtc gaggagctcc gcggcctcgg ctacgacgag gtcgtctccg
     7801 tgcaccacta cgaggacgcg ctgcggcggg ccgtcatcga gctcacggcg acgggcgacg
     7861 ctcccgcgcc gctcatctcg cccgtctgcc ccgccgtcgt gaacctcgtc gaggtcaagt
     7921 tcccgtcgct cctcgagcac ctggtgccgc tcgcctcgcc gtgggaggcc gcccgccagg
     7981 acctcgacgg ccgcgccgcg acgttcgccg tctcgtgccc cagccagcgc accgcgctgc
     8041 tcgcgcagcg ccccaccgcg cagcgggacg cggtcaccgc ggcggccgtg cgcgaggccg
     8101 tgctgccgcg cctcgcggcg cgcgcgcggc ggacgcccgg cgccacggcg tcgccgccgg
     8161 cggcgggcgg cgccgacgac ctgctcgtgg tcaccggtgt cgcgcacgtg ctcgccgtgc
     8221 tcgaggccgt cgaggacgac cgcctgcccg gcgtgtcggc gatcgagccg tacatctgcg
     8281 acggcggctg tttcggctcg ccgctgctcg gcgaggacgc cagcgtggcg gcctggcgct
     8341 ggtcggcggt gggcggcgac gccccgggcg acggtggtac ccacgagcgc tcgcgcccgt
     8401 tccgcgcgcg cccgggcatc cgcctggacg ccgacatggc ggccgccatc cgcaagctgg
     8461 cccagctcga cgccgagacc cgggccctgc ccggcagaga ctgcggcgtg tgcggcgccc
     8521 ccac
//
LOCUS       DASUMX010000033         3214 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_153260, whole genome shotgun sequence.
ACCESSION   DASUMX010000033 DASUMX010000000
VERSION     DASUMX010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3214
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153260"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /gene="hemG"
                     /locus_tag="VFZ86_01420"
     CDS             <1..540
                     /gene="hemG"
                     /locus_tag="VFZ86_01420"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HEX5640977.1"
                     /translation="ATASVAFRAEQVGHDLKGFGFVVPRSEDRPVMATTWSSSKFDGR
                     APDGHVLLRSFLGRAGREAAAQLDDDEMAAVVRSELRDIMGISAEPEFVEIFRWPRGM
                     PQYRVGHVELVAHIEAAVARVPGIELAGGAYHGIGIGDCLREGSAAAERALEHVRGLP
                     EDEIPPQPPDLPAADGLED"
     gene            856..1062
                     /locus_tag="VFZ86_01425"
     CDS             856..1062
                     /locus_tag="VFZ86_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5640978.1"
                     /translation="MTTAQTIAVTIGVVVWLVCLWLVWRMVDRKDLNRALWIILCIFF
                     PVIILIVVALLPAKQRPEPPEFIS"
     gene            complement(1207..>3214)
                     /locus_tag="VFZ86_01430"
     CDS             complement(1207..>3214)
                     /locus_tag="VFZ86_01430"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HEX5640979.1"
                     /translation="TTGVDAGLMLIRKLAFSNDAVTRRILNNSVVMINACQNPDGRIT
                     DLRQNANGFDLNRDWVVQTQPEVRAVAQQIVKWHPTLFLDTHGYYNPMIVDPTTNPHN
                     PNYEWDLSLKWALPTALAMEKAIEDNSIVEVDIPYRDWINPDTGKSEGFEDYSPYYTP
                     QFAMYYGLVGSTMETSFKSEDGVDAHYYGTLEGAKFAASNRVGMLKTQLARFIRGEKG
                     ISQPATDDIPSPLDYPHAYVIPVADGLQADPLQARRAVRHLIRNGIKVYEAKAAFTVP
                     APVGAAAKAAGDTAYPAGTFIVPLQQPLRGIANALLWAGEDVSDRANEMYDAAAWQLP
                     ESWGFTRQAVEEPFSAAMVRVRTATDAAGAVVGAGPTYMLASTTNNAVKAANALLAQG
                     VFVKRVVAESATVPLGAYIVTASQARVRDVLVANAKRYKVDFLTADLAGVTTEQLYTA
                     DRRGAMTGVLKVGVYSDDPTWYALKQLGFDAERMAATADLSAYDVIVVEDPSGLDIDA
                     LKAWIADGGAYIANGPYGILDGVLPVTPMGGYNAAFYWANNTLGLTAHADGSLVTAGH
                     DPYGYTFAFPITWFQVDDPAAVAVDAAYATDYFLAGYWQIGDPTGWVQADAAGQPIVV
                     HGFYGSGRVTFFGPMTAFRAGTEATFRLLSNAIYTASYDDRTDR"
BASE COUNT          437 a         1077 c         1165 g          535 t
ORIGIN      
        1 gcgaccgcct ccgtcgcctt ccgcgcggag caggtggggc acgacctcaa gggcttcggg
       61 ttcgtcgtgc ctcgctccga ggaccggccg gtgatggcga ccacctggag ttccagcaag
      121 ttcgacgggc gcgcgcccga cggtcacgtg ctgctgcgca gcttcctcgg gcgcgccggg
      181 cgcgaggccg cggcccagct ggacgacgac gagatggccg cggtcgtccg ctccgagctc
      241 cgcgacatca tgggcatcag cgcggagccc gagttcgtcg agatcttccg ctggccgcgc
      301 ggcatgccgc agtaccgcgt cggccacgtc gagctggtcg cgcacatcga ggccgccgtg
      361 gcgcgcgtgc ccggcatcga gctcgccggc ggcgcctacc acggcatcgg catcggcgat
      421 tgcctgcgcg agggctcggc cgcggccgaa cgtgctctcg agcacgtccg cggcctcccc
      481 gaggacgaga tccctccgca gccgccggac ctccccgccg ccgacggcct cgaagactag
      541 cgtcaggccc tcgcgcgccc tcgcgctgcc ctggtcgccg tgccgcgccc cgcgctggcc
      601 gagttgcccg acgcgtgatc accggctggc aatccgtcgg cccatggtcg ctcgggactc
      661 gcaccgttgg tcgtacgcca cggagcacaa cgttacatct gcgcgtacgt ccggacgtgc
      721 ctgtccgttt cgtgcaatgg tcctggacgg cggtcgcaag ccgggtgcag cgcaaagggt
      781 tcccggccct catgaggtac acctacgctg agcgatcatg ccgccgccgg cggcccgacg
      841 ccgaggaggc gatccatgac cacagcgcag accatcgcgg taacgatcgg ggtcgtggtc
      901 tggctggtct gcctgtggct ggtgtggcgc atggtcgacc gcaaggacct caaccgcgcc
      961 ctctggatca tcctctgcat cttcttcccg gtgatcatcc tgatcgtcgt ggcgctgctg
     1021 ccggcaaagc agcggccgga gccgccggag ttcatctcct gacggcggcg tgatctgcac
     1081 gcggaagggg gcgggaggcg ctgtgcgcct cccgccccct tcgtcaccca ccgtccgtcg
     1141 gcgctctgcg cccgggcgag tgagcgtcgc gcggcggccc agggggaccc taagccgccg
     1201 ccgcgcctac cggtcagtcc tgtcgtcgta gctggccgtg tagatggcgt tggagagcag
     1261 ccggaaggtc gcctcggtgc ccgcccggaa ggcggtcatg ggtccgaaga acgtgacgcg
     1321 gccgctcccg tagaagccgt ggacgacgat cggctgaccg gcggcgtcgg cctggaccca
     1381 gccggtcggg tcgccgatct gccagtagcc ggccaggaag tagtccgtcg cgtaagcagc
     1441 atcgacggcg accgcggccg ggtcgtcgac ctggaaccac gtgatgggga aggcgaaggt
     1501 gtagccgtac ggatcgtggc cggcggtcac caggctgcca tcagcgtgcg cggtcaggcc
     1561 gagggtgttg ttggcccagt agaaggcggc gttgtagcca cccataggcg tcacggggag
     1621 gacgccgtca aggatgccgt agggaccgtt ggcgatgtag gcgccgccgt ccgcgatcca
     1681 ggccttgagt gcgtcgatgt cgaggccgct cgggtcttcg acgacgatga cgtcgtaggc
     1741 cgacagatcg gcagtggccg ccatgcgctc ggcgtcgaat ccgagctgct tgagggcgta
     1801 ccaggtcgga tcgtcgctgt agacgccgac cttgagcacg ccggtcatgg cgccacggcg
     1861 gtctgcggtg tagagctgct cggtggtgac gcccgcgagg tcggcggtca ggaagtcgac
     1921 cttgtagcgc ttcgcgttgg ccacgagcac gtcgcgcacg cgcgcctgcg acgccgtcac
     1981 gatgtacgcg ccgagcggga cggtcgcgct ctccgcgacg acgcgcttga cgaacacgcc
     2041 ctgggcgagc agtgcgttgg ctgccttgac cgcgttgttg gtcgtactgg cgagcatgta
     2101 cgtggggcct gcgccgacca ccgcacccgc ggcgtcggtc gccgtgcgca cgcggaccat
     2161 ggcggccgag aacggctcct ccaccgcctg gcgcgtgaat ccccaactct cggggagctg
     2221 ccaggcggcg gcgtcgtaca tctcgttggc ccggtcggag acgtcctcgc ccgcccagag
     2281 cagcgcgttg gcgatgccgc gcagcggctg ctggagcggc acgatgaacg tgccggccgg
     2341 gtaggccgtg tcgccggcgg ccttggctgc ggcgccgacc ggagccggca cggtgaaggc
     2401 ggccttcgcc tcgtacacct tgatgccgtt cctgatgagg tggcgcacgg cgcggcgcgc
     2461 ctgcagcgga tcggcctgca ggccgtccgc caccgggatc acgtaggcgt gcgggtagtc
     2521 cagcggcgag gggatgtcgt cggtggccgg ctgcgagatg cccttctccc cgcggatgaa
     2581 gcgggccagc tgggtcttga gcatgccgac gcggttgctc gccgcgaact tggccccctc
     2641 cagggtgccg tagtagtggg cgtcgacccc gtcctcactc ttgaaggagg tctccatggt
     2701 ggagcccacg agaccgtagt acatcgcgaa ctgcggcgtg tagtacgggc tgtagtcctc
     2761 gaagccctcg ctcttgccgg tgtcggggtt gatccagtcg cggtagggga tgtcgacttc
     2821 gacgatggag ttgtcctcga tggccttctc catggccagc gcggtcggca gcgcccactt
     2881 gagcgacaga tcccactcgt agttggggtt gtgggggttg gtcgtcggat cgacgatcat
     2941 cgggttgtag tacccgtgcg tgtcgaggaa cagcgtcggg tgccacttga cgatctgctg
     3001 cgccacggcc cggacctccg gctgtgtctg cacgacccag tcgcggttga ggtcgaagcc
     3061 gttggcgttc tgacgcaggt cggtgatgcg cccgtccggg ttctggcagg cgttgatcat
     3121 gacgacgctg ttgttgagga tccgccgcgt cacggcgtcg ttggagaacg ccagcttcct
     3181 gatcagcatg aggccggcgt cgacgcccgt ggtc
//
LOCUS       DASUMX010000034         5609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1016699, whole genome shotgun sequence.
ACCESSION   DASUMX010000034 DASUMX010000000
VERSION     DASUMX010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 5609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5609
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1016699"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="VFZ86_01435"
     CDS             <1..238
                     /locus_tag="VFZ86_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HEX5640980.1"
                     /translation="EGSLDGVPMTPHPVFGVDVPSSCPGIPDQLLDARSTWADPAEYD
                     LRAATLARQFAANFEAFSGRVSPEIAAAGPRTAG"
     gene            319..897
                     /locus_tag="VFZ86_01440"
     CDS             319..897
                     /locus_tag="VFZ86_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5640981.1"
                     /translation="MAAAALLAACGGQTAAPIDQESHGPSPAGPSPAASAAFRPAVII
                     SDRAGPSWKGRLGETVQIDWYDQRRDEMHSEPITVLSVKRLPNPDDDGGPDEFGDGYG
                     PYEWKYGIKVRLTSLDEHSARRPAAYQFIQLSDGRDSENGVSGLGRPGGPDPSRPGRS
                     SVGWLSQMAEQGFTPTQVTLSVGAWSATWSLD"
     gene            1192..2388
                     /locus_tag="VFZ86_01445"
     CDS             1192..2388
                     /locus_tag="VFZ86_01445"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5640982.1"
                     /translation="MRGRTFGLGAAMLAVVVVATLAIAIAGCGGDENDTGDGGSQEPI
                     KIGAILSLTGTYAGLGEPEKNLIEMEVKKINDAGGINGRPVEVIIEDDATDEAQAVAA
                     ASRLIEQENVVAIIGATGTGQTMAIRGDVQRAGIPQVSMAGGTVITNPVDPQVFATPW
                     SNTIVVPFTLDYLKSQGITKIGLITDSGGFGKDGQAVFATEAPKAGAEIVADETFNPG
                     DTDMTAQLTKIKNSDAQAIVMWTAGSEAAIIAKNAQDLGIDLPVYGSHGIARREFIDG
                     AGEAANGVKFAAGKILIPEAYGEDTEAYAVATAFIDEYTQAYGEAPNTFAGHAYDALY
                     LVVEAAKRIEGDITPEALRDELENTAGFVGIGGTFNLSPDDHNGMTQDDLSMYEISGG
                     DWTLAQ"
     gene            2404..3276
                     /locus_tag="VFZ86_01450"
     CDS             2404..3276
                     /locus_tag="VFZ86_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009493049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX5640983.1"
                     /translation="MDQLLQVLVAGLKNGSIYALVALGFTIVYAATGAINFAQGEFFM
                     LGGMLCVSFTELGLPLPAAAVAAIATTAAIGAVFELLAVRPVAGDDPLRIIMVTIGGA
                     VVLRQLALHIFGPDERALAPFTEGASLKALGVAVELQTLWVWGLTAIAVVALALLYRK
                     TSFGRAMRATAIRTDAARLVGVNTGMMVTVAFALAAGLGALAGVAVAPLTQTAFFVGA
                     AIGVKGFAAAILGGIGKPISAVAGGLLLGLLESFAAGYLDPAYKDGVALVVLLVVLFV
                     APQGLLGRARRVKA"
     gene            3318..4256
                     /locus_tag="VFZ86_01455"
     CDS             3318..4256
                     /locus_tag="VFZ86_01455"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX5640984.1"
                     /translation="MAVALVVPLVVSDRFLLKVFTFCGVNALVVVGLALLFGYAGQIS
                     LGNAAFVGLGAYTSAFCTVELGWPWLLGFAAAGAVAALGGLALAIPSLRLKGHYLAMA
                     TLGFGLLMSLAFLEAEPITGGVDGFTGIPFPSIGPVEFRSASSLYWLVWGCVGVAVLV
                     AHNITSLRPGRAMRALHGSELGAQACGVDIVGVKVRTFVVSALMAGLAGALYAGVVGF
                     ISPSLFTLTASVTFIAMTIVGGTSSLAGPIVAAVALTLVQYVDALIPGLPRETAQTIQ
                     SYQEDIYGIAIVLIVIFAPRGLAGLWKRRNARNEAS"
     gene            4253..5026
                     /locus_tag="VFZ86_01460"
     CDS             4253..5026
                     /locus_tag="VFZ86_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5640985.1"
                     /translation="MTLLQVRNVTRRFGGLTAVEDVSFDVFEGTIKALIGPNGAGKST
                     LFNALTGFDRPDEGSVLFEGEELVGRRPRDTVRAGVARTFQNTQLFEEMTAGENVMVG
                     SQARQRRGFTIAMLRLPSAIAEDRDAREEASRLLRLIGIEEWVDVPAADLPAGIRRLV
                     EIARALATRPRLLLLDEPAAGLNATETRELVQTLFRVRDSGITVGIVEHDMGLVMEVS
                     DEIVVLDRGAKIAEGPPRMIQKDPAVIAAYLGGEVDDDV"
     gene            5019..>5609
                     /locus_tag="VFZ86_01465"
     CDS             5019..>5609
                     /locus_tag="VFZ86_01465"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5640986.1"
                     /translation="MSEAATLLELTSIRAGYGHAEVLHDIDLTVGEGELIAVIGANGA
                     GKSTLLKAIVGLVPIRSGTIAYRGARIAAPRPERLVRKGLALVPEGRLLFGPMTVREN
                     LELGAYGVGRDRRAVAERALARVNALFPVLEERAGQPAETLSGGEQQMLAVGRALMSE
                     PRLLLLDEPSLGLSPLLTTEIFRAIVRINEEQGTTIL"
BASE COUNT          807 a         2015 c         1939 g          848 t
ORIGIN      
        1 cgaggggtcc ctggacggcg tcccgatgac cccgcatccc gtcttcggcg tggacgtgcc
       61 gtcctcgtgc ccgggcatcc cggaccagct cctcgacgcc cggtccacct gggccgaccc
      121 ggcggagtac gacctgcggg cggcgacgct ggcgaggcag ttcgccgcca acttcgaggc
      181 gttctccggc cgcgtgtccc ctgagatagc ggcggctggc ccacggacgg ccggctgacc
      241 tcggcggcgt cgcccacggc tcgcctgacc gcgctcgctc gcgggacggc ggcatccgga
      301 cgcacgctcg cgctcgcagt ggcggcggcc gcccttctgg cggcctgcgg cggccagacc
      361 gcggcgccca tcgatcagga atcccacggc ccgtcgcccg ccggcccgtc gcccgccgcc
      421 agcgccgcct ttcgccccgc agtgatcatc tcggaccgcg ccgggccgtc gtggaagggc
      481 cggcttggcg aaacggtcca gatcgactgg tacgaccagc gcagagacga gatgcacagc
      541 gaaccgatca ccgtcctctc cgtgaagcgc ctcccgaatc cggatgacga cggtggaccg
      601 gacgagttcg gtgacggcta cggcccctac gagtggaagt acggcatcaa ggtgcggctc
      661 accagcctcg acgagcactc ggcccggcga ccggccgcgt accagttcat ccagctcagc
      721 gacgggcgcg actcggagaa cggggtgtcg ggactcggcc ggcccggcgg cccggaccct
      781 tcgcggccgg gaaggagctc cgtcggctgg ctctcccaga tggcggagca gggcttcacg
      841 ccgacgcagg tgacgctctc ggtcggcgcc tggagcgcca cctggtcgct ggactgatcg
      901 ccgccgcggg agcagccggt ctttcacccc catcgcgccc gtgccgtgct atcatgcgcg
      961 aaccttttgc gccgggtctc acagcccggc gagggggggt cgcgcgagcg ccgaccgttc
     1021 tcgcttctct ggcagccgcg cgcgcgacgt gcgcgtgccg taacggcgcc ccgaggcgca
     1081 cggacgcgct ctgcgacacc caagctcgcg gcagggcttt cgcgaccagg gtctcagcgg
     1141 accgcgccca ccgctccgcg gcgcacatgc gaacatgatg ggggtagtgt gatgcgcgga
     1201 cgcacattcg gactcggggc ggccatgctg gccgtcgtag tggtcgcgac gctggcgata
     1261 gccatcgccg gctgcggggg ggacgagaac gacaccggcg acgggggatc ccaggaaccg
     1321 atcaagatcg gcgcgatcct ctctctgacg ggcacgtacg ccggtctcgg cgagcccgag
     1381 aagaacctca tcgagatgga ggtcaagaag atcaacgacg cgggcggcat caacggccgg
     1441 cccgtcgagg tgatcatcga ggatgacgcc accgacgagg cccaggcggt cgctgcggcc
     1501 agccggctca tcgagcagga gaacgtcgtc gccatcatcg gcgccaccgg caccggccag
     1561 accatggcca tccgcggcga cgtgcagcgc gccggcatcc cgcaggtctc catggccggc
     1621 ggcaccgtca tcaccaaccc ggtggacccg caggtgttcg ccacgccgtg gtccaacacc
     1681 atcgtcgtcc cgttcacgct ggactacctg aagagccagg gcatcaccaa gatcggcctg
     1741 atcaccgaca gcggcgggtt cggcaaggac ggccaggctg tcttcgcgac tgaggccccc
     1801 aaggccggcg ccgagatcgt cgccgacgag accttcaacc ccggcgacac cgacatgacg
     1861 gcgcagctca ccaagatcaa gaactccgac gcccaggcga tcgtcatgtg gacggccggc
     1921 tcggaggccg cgatcatcgc caagaacgcc caagacctcg gcatcgacct gccggtctac
     1981 ggcagccacg gcatcgcccg gcgggagttc atcgacggcg ccggcgaagc cgcgaacggc
     2041 gtcaagttcg cggccggcaa gatcctgatc ccggaggcgt acggcgagga caccgaggcc
     2101 tacgcggtgg ccaccgcctt catcgacgag tacacgcagg cctacggtga ggcgcccaac
     2161 acgttcgccg gccacgcgta tgacgcgctc tacctggtcg tggaggccgc gaagcgcatc
     2221 gaaggcgaca tcacgcccga ggcgctgcgc gatgagcttg agaacaccgc cggcttcgtg
     2281 ggcatcgggg ggacgttcaa cctctcgccc gacgaccaca acgggatgac acaggacgac
     2341 ctctcgatgt acgagatctc gggcggcgac tggacgctgg cgcagtaagc cgcggcgcgc
     2401 tccgtggacc aactcctgca ggtcctcgtc gcgggcctca agaacggctc gatctacgcg
     2461 ctggtcgcgc tcggcttcac catcgtctac gcggccaccg gggcgatcaa cttcgcccag
     2521 ggggagttct tcatgctcgg cggcatgctg tgcgtgtcgt tcaccgagct cggcctgccg
     2581 ctgcccgcgg ccgcggtggc ggccatcgcc accaccgcgg ccatcggcgc agtcttcgaa
     2641 ctgctcgccg tgcgccccgt tgccggcgac gatccgctgc gcatcatcat ggtcaccatc
     2701 ggcggcgcgg tcgtcctgag gcagctagca ctgcacatct tcgggcccga cgagcgggcg
     2761 ctcgcgccgt tcacggaggg tgcctcgctc aaggcgctcg gggtagcggt ggagctgcag
     2821 acgctgtggg tctgggggct cacggccatc gccgtcgtcg cgctggcgct gctctaccgc
     2881 aagacctcct tcgggcgagc gatgagggcc acggcgatcc ggaccgacgc cgcgaggctc
     2941 gtcggcgtca acaccggcat gatggtcact gtggcgttcg cgttggccgc gggactcggc
     3001 gccttggccg gcgtggccgt ggcgccgctc acgcagacgg cgttcttcgt aggcgcggcg
     3061 atcggcgtca agggcttcgc cgcggccatt ctcggcggca taggcaagcc catctcggcc
     3121 gtggccggtg gcctgctgct cggcctgctc gagagcttcg cggccgggta tcttgacccg
     3181 gcctacaagg acggcgtggc gctcgtcgtg ctcctcgtcg tcctcttcgt cgccccgcag
     3241 ggtctgttgg gcagggccag acgagtcaaa gcctgatgtc cggacgaacg ctcagggcgc
     3301 ttgctcccct cgtcgcggtg gcggtcgcgt tggtggtgcc gctggtggtg agcgaccggt
     3361 tcctgctcaa ggtgttcacc ttctgcggcg tcaacgccct cgtcgtggtc ggcctcgcgt
     3421 tgctgttcgg ctacgcgggt cagatctcgc tggggaacgc cgccttcgtg ggcctgggcg
     3481 cctacaccag cgccttctgc accgtggagc tcggctggcc gtggctgctg ggcttcgcgg
     3541 ccgccggcgc cgtggcggct ctcggcggcc tcgctctggc gatcccgagc cttcgcttga
     3601 agggccacta cctggcgatg gccacactcg gcttcggcct gctcatgtcc cttgcgttcc
     3661 tcgaggcaga gcccatcacc ggaggcgtgg acggattcac cggcatcccg ttcccgtcca
     3721 tcggcccggt tgagttccgc tcggcctcgt cgctgtactg gctggtgtgg ggctgcgtcg
     3781 gcgtcgccgt gctggtggct cacaacatca cctcgctgcg accaggcagg gccatgcgcg
     3841 cccttcacgg tagcgagctg ggcgcgcagg cctgcggagt ggacatcgtg ggtgtcaagg
     3901 tccgcacgtt cgtcgtgagc gcgctcatgg cgggcctcgc cggcgcgctc tacgcaggcg
     3961 tggtcgggtt catctcgccg agcctcttca ctctgacggc gtcggtcacg ttcatcgcca
     4021 tgaccatcgt cggagggacc agttcgctcg ccgggccgat cgtcgccgcc gtcgctctca
     4081 ccttggtgca gtacgtggat gcgctcatcc ccggactgcc gcgcgagacg gcgcagacca
     4141 tccagtcata ccaggaagac atctacggca tcgccatcgt gcttatcgtg atcttcgcgc
     4201 cgcgcggcct cgccggactc tggaagcggc gcaacgccag gaacgaggcg tcatgacgct
     4261 gctgcaggtg cgcaacgtga cgcgccgctt cggggggctc accgccgtgg aggacgtctc
     4321 cttcgacgtg ttcgagggca ccatcaaggc gctcatcggc cccaacggcg cgggcaagtc
     4381 caccctgttc aacgcgctca cggggttcga ccggcctgac gaaggctcgg tgctgttcga
     4441 gggcgaggaa ctggtcggcc ggcgcccacg cgacaccgtg cgcgccggcg tggcgcgcac
     4501 cttccagaac acgcagctct tcgaggagat gaccgccggc gagaacgtga tggtcggcag
     4561 ccaggcgcgt cagcgccgcg gcttcacgat cgcgatgctg cggctcccct cggcgatagc
     4621 cgaggaccgc gacgcgcgtg aagaggccag ccggctgctg cggctcatcg gcatcgagga
     4681 gtgggtggac gtgccggccg ccgacctccc cgccggcatc cgccgtctcg tcgagatcgc
     4741 ccgggcgctg gcgacacgcc cgcggctgct gctgctggac gagccggccg cgggcctcaa
     4801 cgcgaccgag acgcgcgagc tcgtccagac gctcttccgc gtccgggact cgggcatcac
     4861 cgtcggcatc gtcgagcacg acatgggcct cgtcatggag gtctccgacg agatcgtggt
     4921 gctcgaccgc ggcgccaaga tcgcggaggg tccgccgcgg atgatccaga aggacccggc
     4981 cgtgatcgcc gcctacctgg gcggcgaggt agacgacgat gtctgaggcc gccacgctgc
     5041 tggagctcac gtcgatcagg gccggctacg gccacgccga ggtgctgcac gacatcgacc
     5101 tcaccgtcgg agagggcgag ctcatcgccg tgatcggcgc gaacggcgcc ggcaagtcga
     5161 ccttgctcaa agcgatcgtc ggcctcgtcc ccatccgctc ggggacgatc gcctaccgcg
     5221 gcgcccgcat cgccgccccg cggcccgagc gcctcgtgcg caagggcctc gcgctggtgc
     5281 ctgagggaag gctcctcttc ggccccatga ccgtccgtga gaacctcgag ctcggcgcct
     5341 acggagtcgg ccgcgaccgg cgcgccgtcg ccgagcgggc cctggcccgg gtcaacgcgc
     5401 tcttcccggt gctggaggag cgcgcgggcc agccggccga gacgctctcc ggcggcgagc
     5461 agcagatgct cgccgtcggt cgcgcgctca tgtcggagcc acggctgctg ctgctcgacg
     5521 aaccgtcgct cggcctctcg cccctcctca ccaccgagat cttccgggcg atcgtgcgca
     5581 tcaacgagga gcagggcacc acgatcctc
//
LOCUS       DASUMX010000035        25884 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1879489, whole genome shotgun sequence.
ACCESSION   DASUMX010000035 DASUMX010000000
VERSION     DASUMX010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 25884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25884
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1879489"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="VFZ86_01470"
     CDS             complement(<1..605)
                     /locus_tag="VFZ86_01470"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5640987.1"
                     /translation="MSRRKWMAVALVALIVMVAAAVALGGCGDDAETTTDDASSPAAA
                     GGTIKVGFVNSATGVSAAPNQAIVGATMGAIEETNAKGGIDGKWQIEVVTKDDGSDVA
                     KASAAVTQLIQQEKVDFILQPWVDFIAPVARGIEEKAGMPGVLSYHPAKGDTGTYKWT
                     FSNAQGAEDNADALVKIFAQQGWKTVVGAGDVLTIQQETLD"
     gene            complement(653..3421)
                     /locus_tag="VFZ86_01475"
     CDS             complement(653..3421)
                     /locus_tag="VFZ86_01475"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5640988.1"
                     /translation="MSNGAEPQVAAGQDATAQQPAAQAAPARRAGFFSAATVAKLVII
                     VILAVWPFFYQDLYTLSWMTFAGLSLMVVVSVYLIIAQAGQLSFGHAAFYGVGAYTAA
                     ILSVRYNVPTLLALLAGAALAGVIALVIGRPVLRLRYFYLALATIGLGIIFSVIVIQA
                     AGITGGTLGLAPVPPLDLFGFKIDTYFRQYYLVWIVALLILLFTERALGMRLGRALRA
                     IATSEIAAETLGVRTANWKLIAFVASAIFCAIAGALYAFVLAAITPSAFAFSAAILPI
                     IMMLIGGGGSIWGGLVGAVLMTWISNSLSGTQQWSGVIYSVIMILLLLFLPMGITGLF
                     TKQRLVGVKNVFRRQSRAETIACAAEVESEGSIGACETQVALPTYVPEHGHDATVAET
                     DSGTLMAELKRGAGEMGEELLKVEGVSVDFGGLRAVNQVTMTVRTGQIMALIGPNGAG
                     KTTLFNVISRLQRASEGRVILAGTEITHKSPADSARLGMARTFQNLRIFENMDVLENV
                     LVGCHRHEKAGFISGGLGLPAQRREEKASRLRAMDALRLVGLDGAAHVPAASLPYGQQ
                     RLVEIARALASEPRLLLLDEPAAGMNAAERTYLVDRIRRIRDAGVTVLLVEHDIELVM
                     GLCDWVTVLDYGKRIADGEPDVVQKDEAVIEAYLGVGHEGAREECGDLSGVDVAAMEA
                     EPAEKLLEVRGLSTRYGAIHALRDVSFGVPKGEIVAVLGANGAGKTTLLHTISGVLRP
                     SAGTITYAGADITKLAPARIVSRGVCQVPEGRRLFPSLSVEDNLLLGASGRSSRDSET
                     DDLASIYELFPALAERRLQPAGTLSGGEQQMVAIGRALMGKPDLLLLDEPSMGLAPLA
                     VERIFEALRELNKSGLTMLMVEQNAEMAFSVADRAVVLQTGAVALTGPAAGLRQDERV
                     RECYLGQA"
     gene            complement(3418..4305)
                     /locus_tag="VFZ86_01480"
     CDS             complement(3418..4305)
                     /locus_tag="VFZ86_01480"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX5640989.1"
                     /translation="MSVSQWLQLLISGIVTGAIYALVGLGYVTIYRTSRVVNFAQGSF
                     VMLGALITYSLLNEAGLPFWLSGIVSVVAVVAVAVAMYFLVIAPIIKVSLVSMILVTI
                     GVSFLLEGLALVKWGGYGLGIPSFPGSRTLHFWRLTIEVQNIWVIVIATLLFVGLWVL
                     SNRTRVGKQMTATASDPLSASLSGISTTRMVVLAFAISAAIGAIGGICVGSISPMSYA
                     SGGMLGLAGFIAAIVGGWGSSSGAIVGGLTVGIAESLATGFLPGGYRSAVAYVLLIII
                     LYFRPSGILGKGGAAEAGQ"
     gene            4452..5429
                     /locus_tag="VFZ86_01485"
     CDS             4452..5429
                     /locus_tag="VFZ86_01485"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX5640990.1"
                     /translation="MTTMYDDTTSTGDVRPLSISELESLTDTPRSTIYYYVRDGLLPA
                     AQKAAASRAVYSEVHVEALREIRTLKGDGVPLDEIRERIRPLVERRQAAEPDLVARRT
                     QQTREAILQAAALQFARRGYRRTRVGDIIKEVGITPPVFYAHFTTKRQLFIEAFNVFV
                     HWMGDLIEPPLADEPDPAVRLLMRFYAYWGLQRLSPDLLGLARAEALQEDGETRAAVQ
                     EAQRWITGGPARDLAGLRRGPDDPPVSDELMAYSLYGAGEEIFMRASWDDAYSPREIL
                     RAHLFLYLAVDAAYTGRCDTVDRLRSYEPLIDRLVEQGPPIPQPRDASI"
     gene            complement(5441..6301)
                     /locus_tag="VFZ86_01490"
     CDS             complement(5441..6301)
                     /locus_tag="VFZ86_01490"
                     /inference="COORDINATES: protein motif:HMM:NF018777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EthD domain-containing protein"
                     /protein_id="HEX5640991.1"
                     /translation="MIKFQLISRRKPGDSQEKYFYEWGTIHVALMITTPSVMRTFKRY
                     AQHFTVNGLDGSRLLYPFSDMAWDNFADHWLERPEDNLVPFRSDDYMQRMHPHDFGDS
                     SFVIEYLTETLRDEAPGFRPGGVKLIHVVKSRPDLSESEFAAAWKDGHAAALLTAGGV
                     RPGRLVQNLHAEVDKKTFEGTLFALAGVGGYAGIEELWFEDLDALLAFTQDSGVRAAV
                     LSEDAPVDPSGSFSMVTTERVVYDYTLGEASSPVAAVLDPASLEARIDAQKYSGWNLP
                     GRERRAKEPA"
     gene            complement(6318..8015)
                     /locus_tag="VFZ86_01495"
     CDS             complement(6318..8015)
                     /locus_tag="VFZ86_01495"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX5640992.1"
                     /translation="MTSLENLGTVLKADLLIVGGGIAGLACAISAKEADPEVDVLIVD
                     KVVTGWGGKANKGGGNIVFVDPAELGNVLDFHVQNVGDFLEDQDLLMDFARETRGSLE
                     KLESWGVHIYRDDDGEPRYVRWLPQHPWRLAVLDQDLTLNMARHARKLGVRIQDRVEI
                     VDLLKDGDRVCGAIGFGMIDGGCVIAEAGAVVLANGNQCYKLMPRWSSAGGEGIAAAY
                     RAGAAMRGAEFGNFINWVFADTKEVCQGAEDCMYNAKGENISKRVRPELESDVHSKEV
                     VEWWREMKAGNGPIVANMIENYVNNEIVPAFHADALAVRPVSMKFWGLTIGKAMAAST
                     VHGPMQPVMPGLNAELAPVKVDHEMATTVPGLFAIGDTCYAGTCLAGAVPAPPGRMRG
                     SGLGFATFSGVRCAPVAVAEARAASGRPDAAQAEALKARVFAPLGRSGMAAPEMVRAV
                     QDVMAPVGYSIYKHAERMNEALGKVLALKEQVPTLAAADPHYLAACHVAESMVLGAEM
                     FYRASLAREESRGWHLREDFPERDDASFLKWIELKDAGGEMTLSYEDVPVADYPLQP"
     gene            complement(8017..8238)
                     /locus_tag="VFZ86_01500"
     CDS             complement(8017..8238)
                     /locus_tag="VFZ86_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005034076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HEX5640993.1"
                     /translation="MNRIEIDRDACTACQTCYEACFADVYRWDEESDRPVVAYAEDCV
                     GCNKCELECPAECIKVVVDFPGIYWPEVV"
     gene            complement(8235..9191)
                     /locus_tag="VFZ86_01505"
     CDS             complement(8235..9191)
                     /locus_tag="VFZ86_01505"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX5640994.1"
                     /translation="MRALTIGELETESGVSRSTIYFYVRAGLLPPAQKSSPTRALYTD
                     DHVELLDEIRRLKQDGLDIKAIREQVRHRVHSASQSGEDLVARQGEQTRRAILRTAAR
                     EFARNGYRQTRIADIIAELGITPQVLYSHFPTKRDLFAAAYRESLGISMSIIEPRLDG
                     EDDLAVHLLWRMVGDYALQGLNPDLLYLSREAAYDDPETTRELRRAHEHVLSGQMEEL
                     AALRQSPDDPPFSDELVNYALYGAFQTMRLRASWDDRYSRSDVMWTNILLNIAVRKLY
                     DGSIDVAAFEEKYAALVDELAAIQSPDISSLESSLPPREGSE"
     gene            complement(9341..9964)
                     /locus_tag="VFZ86_01510"
     CDS             complement(9341..9964)
                     /locus_tag="VFZ86_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5640995.1"
                     /translation="MKSKTWQDDNPWTHIDWELNDVTSYQLNWFWCNMEKADYLWHPN
                     QHKGFEWWISLEEAGGPLGSVHIAPQTWSDGVKIRPYIRMEKLEDVADEIRDLIKYDH
                     VVIVGAISILGDDVQRDDPVLGYRVHQWQKSDNGCVGMSSAVEARENAADDGLIWAAH
                     ANEEVGNWEVFLPDLVRLYRVITDPEIAPYHSFKVDGVGRKARYAEL"
     gene            complement(10022..10615)
                     /locus_tag="VFZ86_01515"
     CDS             complement(10022..10615)
                     /locus_tag="VFZ86_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HEX5640996.1"
                     /translation="MAERPIFNILISGVGGQGVVLASYVLSRVALAEGYDVKQSEVHG
                     MAQRGGCVTSHLRFGDKVHSALITPGTADVLLSFEQVEALRYVHWLKPGGLLVYNAAR
                     VNPSTVSSGAVEYPEGIDERIATAWPDARRVDASELAARAGTVKAANVVMLGAMASAL
                     PFTPGMLESFIRKSVPPKTVDVNLAAFQLGLEVAVPA"
     gene            complement(10615..12468)
                     /locus_tag="VFZ86_01520"
     CDS             complement(10615..12468)
                     /locus_tag="VFZ86_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006522700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX5640997.1"
                     /translation="MTPPDVAAAEAEPRASELVILSGNEAIARGAWEAGVRFASAYPG
                     TPSTEIMEALARYEDVYAEWAPNEKVAIEAATGAALAGARALATMKHVGLNVGADPFF
                     SSSMIRMAGGLVIVSADDPAMHSSQDEQDNRTYAKFAKLAMVEPSDADEALQFIKAAY
                     EMSERFGTPVLFRTTTRTSHAQGVAKFGERATPAGPLLELERDPSQQLMMPHNAIPRH
                     AAVEQRLLDLAEFAETTALNRVEMGDARAGIVASGAAYGYAKEAFPQASFLKLGMSWP
                     LPRRLIAGFRAQVDKLYVVEELDPFLEENIRLQGVTVDAGKDILPLCGEFTPGLVART
                     LSAAGVPGVDESLLLDVPAADEDLPDRPPTLCPGCSHRGVFVALRRLRAFVSGDIGCY
                     SLGALAPFNAMHCSTCMGAGISMAHGMARVLDENADTKNRPVAVIGDSTFFHSGITSL
                     LDVAYNRSNIVTVILDNRTTAMTGGQENPSMGKTLQGQDARLVDIPALVRSLGITRVR
                     EIDPLNVAETQAALKEEIASDEPSVVIAVSPCVLEYRIKRPAYRVDADLCTGCKVCLR
                     AGCTALSLYRNADGEPRVEIDPAVCSGCGVCSQLCRYEAIRPPLTDDGKDA"
     gene            complement(12465..14657)
                     /locus_tag="VFZ86_01525"
     CDS             complement(12465..14657)
                     /locus_tag="VFZ86_01525"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEX5640998.1"
                     /translation="MPPEVSDALAQARELVETALAAGRPALDEAAAKQVLAAYGVPIP
                     AGGLVHSATEAAGMASSLGVPVAMKATGALIQHKTEKQLVVLDVRTPDEAAETYAALA
                     KRAGDALEAVLVEEMVSGSREFMVGMKRDAAFGPVVAFGLGGTMTEVFRDIALAVTPG
                     AAGDVAGLPDLINGRALLGPFRGQPAVDRAGLVAVIEAVARIAADHPQISEIDVNPLL
                     VRGAQPVAADALMVLSAATPAAPASRAFTPDLEAVLAPRSVAIVGASGDVARWGGSAL
                     QNILAGGFEGAIHPVNPKGGEFFGLPVSPSLQDVPAPPDLAVLAVGARQLPEVIAQCG
                     KAGVRAAVAIAAGFSETGDEGAQAEQALAQTAAEAGVTLIGPNCMGLIANATHLHATG
                     FIALHPRPGNVAIVSQSGNLGVQLSALADRRDLGVRCFIGVGNEAQVTAVDVVEYLAD
                     DEGTSCILAYLEGVDDGRRLFDVATATSLKKPVVVLRGGLTDSGGKAAASHTGAMAGS
                     AAVYEAAARQAGLVTCTSVQEALDLATALANLPLPSGHRIAIVTNGGGAGVLAADEMA
                     RRQLTLVDPPSELLAEIDEILPPFWSRRNPFDMVAMAGGDVASRVLAAVARCPEVDAV
                     VALSVLGVPNAGDEVRGQAATGDYEDFSPWERRYLDGVAALMAETGKPIVNVPDLPVR
                     RAVYTGDHRYAPVVVPTPRAAALVLEQMARYGAWRSRHSHHSRPKDDA"
     gene            14769..15500
                     /locus_tag="VFZ86_01530"
     CDS             14769..15500
                     /locus_tag="VFZ86_01530"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HEX5640999.1"
                     /translation="MVHRGFDDMSLTPGTASESAERYVRALIFWGELGPGDRLPPATE
                     LAARLGISRVTLRIALRSLESTGYLITTRGAHGGSRVSDLQTLQKCWDQWMGEHRDEL
                     DDIFEFRFTVETRLAELAARRRTADELAAMERALAGEHDNRRRASLFQADADIHRSIA
                     AGAHSPRLALAMNAVRADLFLPFDEELLDSTEDDVFATHEDIVTAIREQDPERASAAA
                     TKHVQRVRELVARALEASGIGGGRH"
     gene            complement(15540..17744)
                     /locus_tag="VFZ86_01535"
     CDS             complement(15540..17744)
                     /locus_tag="VFZ86_01535"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX5641000.1"
                     /translation="MADRKQIVTTCKSCHGGCGVIVTVQDGAIVHIQGNPDSLTKGTM
                     CSKGLSSIQHIDNPYRIKYPMKQVGRKGDGKWERITWDEALDTIAGKMKAAIADPGPH
                     TIAISQGTGRGYNRYTMKMARSLGTGNAITPGYVCHSPRLGLYGLVTGYGRLYCDYHG
                     WGGEFPKTQIMWAKQLEISSADSEMAYWFMNSLDYCKNLIIIDPRATAYASRASLWIQ
                     PRVGTDCALAMGMMRHIIEQGLWDREFVEDWTFGFDELRERVRDFTPEKVSDICWIPK
                     EHVTTAAEMWAVDTPGCIQVGSSLERQANCGHTLRAITCLMGLTGNIERPGSMITWVL
                     PETGLIEDFFLELPITDEMKAGIIGGDTYKMGAARTCNPDTMVKAINAGKSPIKVWFS
                     VGGQQIVHLANTNEVVSAIEKVEFMSHADLFMGPMAEAADIVLPAAHWLELDDVYDMH
                     PRFMIEAHNKVIDPPGEAKADAWIFNEIGKRVVPDKWFEDVEALLDYQVRKGKNGTMT
                     WKDFSEQLVSGCYGPDQVYYKYKTDYWHEGGGFPTPSRKFEFHSNHLESLGYDPLPVF
                     REPGESPVSTPELYEEYPLVMSSGFRQPFYFLGQYRNIPWLRSYMEFPQCQMHPETCE
                     KYGVEDGDIVWIESPRGRIRQKLRAFPGIKKDMIMCTANWFYPEEPAKGYHGVFISNP
                     NVLTSNDHLDPMYGSPDLTCLLCKVYKARDEELQEEVFHTEAYGTVPTYGKS"
     gene            complement(17780..18754)
                     /gene="nrfD"
                     /locus_tag="VFZ86_01540"
     CDS             complement(17780..18754)
                     /gene="nrfD"
                     /locus_tag="VFZ86_01540"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEX5641001.1"
                     /translation="MKAQTRRQWITTHEWMVKPMRQKEWISGKGILVWLAEVFSALGM
                     GTYLVGLLAGISEITTWAWWATVLGWVLIVLFKLPFHLAYLGRPLRFWRAIPPFTKAW
                     RTSWLARGMFFSIVFTGAGFLQIVFWYFLRWGDLSASASDALTVLSWIVGIVAGVFIL
                     LTGVYCGFAMSYCKSLPFWNTGLLPIVFVIMGVADGLALTMGVNLITGADEAAISDLE
                     FWSRILLVVNALLIAGYLARAYQRHSAAELAAQDLIKGSTAWVFWVLLVILGIATPLV
                     ISVATAFMEGDTEPLLIVAIVCHTIGAFALKYSVLKVGIYRSVLPGVG"
     gene            complement(18751..19539)
                     /locus_tag="VFZ86_01545"
     CDS             complement(18751..19539)
                     /locus_tag="VFZ86_01545"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX5641002.1"
                     /translation="MRWGMVINLAKCMRCHACVAACRIEHFLPLDITWVKLIAHEVDE
                     DGAVEVSTYPVRCNQCADAPCVHACPTEASHQREDGIVVIDSDKCIGCRYCVIACPYQ
                     NRTFYSKDKDPGYFPGYERTRFEKMGQKLYPHQRGTTEKCNFCVERIDDGMSRGLTPG
                     VDRDASPACVNTCQARALTFGDLDDPDSEVNRLIRRYGGFQLRAEYGTEPSVWYIDYR
                     LGGNDNKAPREAQTGSHIQHLSSVDRDAFRRIYGREPADAEAKP"
     gene            complement(19678..20493)
                     /locus_tag="VFZ86_01550"
     CDS             complement(19678..20493)
                     /locus_tag="VFZ86_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005809159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX5641003.1"
                     /translation="MKAFVIDVARCNGCYGCQFVCKDEHVGNDWAPIAKPQPDAGQFW
                     MRVDEFIRGTVPKVRMHYVPNPCMHCDAAACMEACQVEGAICKRDDGMVVIDTDKCTG
                     CKACVSACPYGSIYFSDDLNLAQKCTGCAHLLDDDEWEYPRCVDSCPTKAITFAEESE
                     LAGLIARAEVMKPEAGTSPRVHYLNLPKKFIAGTVYDPVEKEVVIGATCTLTAADGAV
                     LHVSTDEFGDFWFTDIVAGTYALSIEADGFAAKAYEAVDAAEDVNLGDIALSS"
     gene            complement(20496..23123)
                     /locus_tag="VFZ86_01555"
     CDS             complement(20496..23123)
                     /locus_tag="VFZ86_01555"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX5641004.1"
                     /translation="MSEDARKKVDVKPDKTVIKALSQGGLLGGTGEVMVDVKDGKILR
                     IRPFHFDWKYDGSTFRPWKIERNGAVLEPGLKSRPAPYATAYKKRTYSPNRILYPLKR
                     VDWDPDGERNPQNRGVSKYERISWDEATDIIAKEIVRIQKQYGPNAILVQGDGHGESK
                     TIHTPHGHSGRLLDAMGGFTLQVRQPDSWEGWYWGSKHVWGHGFQGMMAPQLNVGKDI
                     SEHCDMVLKWGCDDETTPWGFTGQNATNISYFWTEAGIKQVYICPDLNYGAAVHADKW
                     IPVLPNSDAALQLAIIYTWLKEDSWDKEYVATHAVGMDKVAAYVLGDEDGIPKSPEWA
                     APKCGVPEWTIKALAREFAAKCTSIVHYFGGSMIRGPYSHEPGRLECILLGMQGLGGP
                     GVHQWQMSYFGMPRAESLDNFRYFNPTLSEHLRVPVQSSVDAWATQNLPKTLIHEAIL
                     HGPLKFTGTGAQNAETEDQFIEYEFPLPKEEGGSELHMIWTDTPCRITCWNHGNWIIE
                     SHRHESIEFMVAQHPWLENDCLYADIILPANTTMEVDDCVTNVRQGPPFQTCTLQDKA
                     IEPVGESRSDYEIVLAVADKLGLKEKITQGKSVRDLQKEVFECMYGSRYMTWEEFEEK
                     KYVVYPTAEDWESDPPGLREFYDDPKASPLKTPSGLLEFYSERLATHFPHDEERPPIP
                     KWVERGVTHDERISSERADLFPLVLMSNHPRWRTHAQGDDCTWARETPTGKVLGPDNY
                     NYEPVWLHPTDAAERGIEHGEIVKIFNERGIVLAGACVWERLMPGVAYIDHGARHDPI
                     IPGKVDRGGAINTIAPNGLTSRQCCGQATSGYLVDVQKIRPEEWETWRRDYPEAFERE
                     YDPGAGLRFDGWVVEGGSR"
     gene            complement(23120..24022)
                     /locus_tag="VFZ86_01560"
     CDS             complement(23120..24022)
                     /locus_tag="VFZ86_01560"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX5641005.1"
                     /translation="MTTTSARATDGQASGRGERSGDVDPLTVRALARGLSILSLYDVD
                     HREWTIDEIAARTGLLRMTAYRMVRTLEAAQYLVRDHATNQYHLGPAAITMAYVAEDT
                     SEFVEHARPYLVRLLDETGESVTLAVPVDGVPVCVSILNSARPFQRRIAPGRIIGDLA
                     SVHGKIFTAFAPPERRAAVLAQQRRRHTPRTVTDPGALERELERVAADDVAFDDEGLY
                     LSVCSVGSPVRDQLGNVVAAISVVMPAGRYGPGERDLCVRAVKDAAASLSAYLGWNPS
                     RPSLESQPDPATGASTKAKGQRLR"
     gene            24215..>25884
                     /locus_tag="VFZ86_01565"
     CDS             24215..>25884
                     /locus_tag="VFZ86_01565"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX5641006.1"
                     /translation="MSEQVFTNLTNCGPVSVYVNDGVVTRIRPLVADEAEFRPWVIEA
                     GGKSYSPPKKFNLAPMVHAERRRLYSDDRIKYPMKRVDFDPDGERNPQNRGKSAYERI
                     SWDEALDIVSSEIARVKKKYGSSAISGMTSSHHNWGIVGYKMGPFMRFMNCLEATPVF
                     DNPDSWEGWHWGATHTYGFYWRLGMPEPFDNLQDVMRNSEMIVFWSNDPDTTRGTYSG
                     QESAIWRTWLKEKGVQAVFIDPFHNYTNAIFADAKWIAPRMGTDTALAMAIAHVWITE
                     DTYDKQYVADRTIGFDEFKPYVLGETDGVPKTPEWAAEESGVAARVIRTLAREWAARP
                     TALAAGARGGEGSACRTAYATEWARMMVLLQAMQGLGKPGVTIGGVSMGAPAEFDTWF
                     PAYAEPMGRMSHSTKAAAYIPQNSTKQRLYRLTVPDAILDGHIEWDGAGFCGQSLEQQ
                     FEHFVYPAEGCSEIKLWYRYGGSFMGTMSDTTKWVRMYQSPKLEFVVNQDIWFNSETR
                     MADVILPACTNFERDDLGEFAACNGYTTNSHSGNNYRVIVREQKCIEPL"
BASE COUNT         3992 a         8667 c         9012 g         4213 t
ORIGIN      
        1 tagtcgagcg tctcctgctg gatcgtcagg acgtcaccgg cgccgacgac cgtcttccag
       61 ccctgctgcg cgaagatctt gaccagcgcg tcggcgttgt cctcagcgcc ctgcgcgttg
      121 ctgaacgtcc acttgtacgt accggtgtcg cccttggcgg ggtggtacga gagcacgccc
      181 ggcatgccgg ccttctcctc gatcccgcgg gccaccgggg cgatgaagtc cacccacggc
      241 tgcaggatga agtcgacctt ctcctgctgg atgagctgcg tgacggccgc cgaagccttg
      301 gcgacgtcgg agccgtcgtc cttggtgacg acctcgatct gccacttgcc gtcgatgccg
      361 cccttggcgt tggtctcctc gatcgcgccc atggtcgcgc cgacgatcgc ctggttcgga
      421 gcggcgctga cgccggtcgc cgagttgacg aacccgacct tgatggtgcc gccggcagcg
      481 gccggcgatg aagcatcgtc cgtggtcgtc tccgcatcgt cgccgcagcc gcccaaggcg
      541 acggccgccg cgaccatgac gatcagcgcc accagcgcta ccgccatcca cttgcgtcta
      601 ctcatcactc ccccttgtct cgtttgctgc cccatcggca atgtctggag ggtcaggctt
      661 gacccaggta acactccctc acgcgctcgt cctgacgcag cccggcggcg ggacccgtga
      721 gggccacggc tccggtctgc aggacgacgg cgcggtcggc gaccgagaac gccatctcgg
      781 cgttctgttc caccatcagc atggtcagtc cggacttgtt cagttctctg agggcctcga
      841 agatgcgctc gaccgcgagc ggggcgagcc ccatggacgg ctcgtccagg agcagcaggt
      901 ccggcttgcc catgagggca cggccgatgg cgaccatctg ctgctcgcct cccgagagcg
      961 tgccggccgg ctgcaggcgc cgttcggcga gcgccgggaa gagctcgtag atggaggcga
     1021 ggtcgtcggt ctcgctgtcc cgtgaactcc ggcccgacgc gcccagcagc aggttgtcct
     1081 cgacggagag cgacgggaac agccgccggc cctcgggcac ctggcagacg ccgcggctca
     1141 cgatcctggc cggcgccagc ttcgtgatgt ccgcgccggc gtacgtgatg gtcccggcac
     1201 tcgggcggag cacacccgag atggtgtgca gcagcgtggt cttgccggcg ccgttggcgc
     1261 cgagcacagc gacgatctcg cccttgggga cgccgaacga gacgtcgcgc agggcgtgga
     1321 tggcgccgta gcgcgtggag aggccacgca cctcgaggag cttctcggcg ggctcggcct
     1381 ccatggcggc cacgtccacc ccgctcaagt cgccgcattc ctctctggcg ccctcgtgac
     1441 caacgccgag gtacgcctcg atgaccgcct cgtccttctg cacgacatcc ggctcgccgt
     1501 ccgcgatgcg cttgccgtaa tcgagaaccg tcacccagtc gcagagaccc atgacgagct
     1561 cgatatcgtg ttcgacgagg aggacggtga ccccggcgtc gcggatgcgg cggatacggt
     1621 ccacgaggta ggtacgctcg gcggcgttca tgccggcggc cggttcgtcg agcaggagca
     1681 gtcgcggctc ggaggcgagg gcgcgggcga tctccacgag ccgctgctgc ccgtagggca
     1741 gactggcggc cggcacgtgc gccgcgccgt cgagcccgac gagccgcagc gcgtccatgg
     1801 cgcgcagccg ggaggccttc tcctcacggc gctgggcagg cagcccgagg cccccggaga
     1861 tgaacccggc cttctcgtgc ctgtggcagc ccacgaggac gttctcgagc acgtccatgt
     1921 tctcgaagat gcgcaggttc tggaacgtgc gcgccatgcc caagcgggcg ctgtcggccg
     1981 gcgacttgtg cgtgatctcc gtcccggcga ggatcacgcg cccctcagac gcgcgctgca
     2041 gccggctgat gacgttgaac agcgtcgtct tgccggcgcc gttcggaccg atgagcgcca
     2101 tgatctgtcc ggtgcggacc gtcatggtca cctggttgac ggcgcggagg ccgccgaagt
     2161 ccaccgagac gccctcgacc ttgagcagct cctcacccat ctccccggcg ccgcgcttga
     2221 gctcggccat gagcgtcccg gagtccgtct ccgccacggt ggcgtcgtgg ccgtgctcgg
     2281 gcacgtaggt cggcagcgcg acctgggtct cgcaggcgcc gatggagccc tcgctctcca
     2341 cctccgccgc gcaggcgatc gtctcggcac ggctctgccg gcggaagacg ttcttgacgc
     2401 cgacgagcct ctgcttcgtg aacaggccgg tgatgcccat gggcaggaag agcaggagga
     2461 ggatcatgat gaccgagtag atgacgccgc tccactgctg ggtgcccgac aggctgttgg
     2521 agatccacgt catcagcacg gcgcccacga gcccgcccca gatggatccg ccgccgccga
     2581 tgagcatcat gatgatcggc aggatggcgg cgctgaacgc gaaggccgag ggcgtgatgg
     2641 ccgccagcac gaaggcgtag agcgcgccgg cgatggcgca gaagatggcg ctggcgacga
     2701 aggcgatgag cttccagttg gcggtgcgca cgccgagcgt ctcggcggcg atctcgctgg
     2761 tcgcgatggc gcgcagggcg cggccgaggc gcatgccgag ggcgcgctcc gtgaacagca
     2821 ggatgagcag ggcgacgatc cacaccaggt agtactggcg gaagtaggtg tcgatcttga
     2881 acccgaagag gtcgagcggc gggaccgggg cgagcccgag ggtgccaccg gtgatgccgg
     2941 cggcctggat cacgatgacg ctgaagatga tgcccaggcc gatggtggcg agcgcgaggt
     3001 agaagtagcg caggcgcagt accggccggc cgatgaccag ggcgatcacg ccggcgaggg
     3061 cggctcccgc cagcagcgcc aggagcgtcg gcacgttgta gcggacagag aggatggccg
     3121 ccgtgtaggc gccgaccccg taaaaggcgg cgtggccgaa cgagagctgg cccgcctgcg
     3181 cgatgatcag gtagacgctc acgacgacca tcagcgacag gccggcgaag gtcatccagc
     3241 tgagcgtgta caggtcctgg tagaagaagg gccagacggc caggatgacg atgatgacca
     3301 gtttggcgac cgtcgctgcg gagaagaacc ctgcgcggcg agccggagca gcctgcgccg
     3361 ccggctgctg cgccgtcgcg tcctggccgg cggccacctg cggctctgcc ccgttgctca
     3421 ctgtccggcc tccgccgcgc cgcccttgcc gaggatcccc gaaggccgga agtacaggat
     3481 gatgatcagc agcacgtagg ccacggcgct gcggtacccg ccgggcagga acccggtggc
     3541 cagcgactcg gcgatgccga ccgtcagacc gccgacgatg gcgccgctgc tcgagcccca
     3601 gccgccgacg atggcggcga tgaacccggc gaggccgagc atgccgccgg aggcatagct
     3661 catcggactg atggacccca cgcagatgcc gccgatggcg ccgatggctg cgctgatcgc
     3721 gaacgccagc accaccatgc gagtggtcga gatgccggag aggctcgcgg agagcgggtc
     3781 gctcgcggtc gcggtcatct gcttgccgac cctggtccgg ttggaaagca cccacagccc
     3841 gacgaagagc agcgtggcga tgacgatgac ccagatgttc tgcacctcga tggtcagcct
     3901 ccagaagtgc agggtacggc tgccggggaa gctggggatg ccgagcccgt agccgcccca
     3961 cttcaccagc gcgagaccct ccagcaggaa gctcacaccg atggtgacca ggatcatcga
     4021 gaccagcgag accttgatga tgggagcgat gacgaggaag tacatggcga ccgccacggc
     4081 gaccacggcc acgacggaga cgatgccgga gagccagaac ggcagaccgg cctcgttgag
     4141 caatgagtac gtgatgagcg cgcccagcat gacgaacgag ccctgggcga agttcaccac
     4201 gcgtgacgtg cggtagatgg tcacgtagcc gagcccgacc agcgcataga tcgcgccggt
     4261 gacgatcccg gagatcaaca actgcagcca ctggctgacg ctcatcctgc cccccagcaa
     4321 gaggtgagag tgacccgccg gctcggtccc agcaggcaac tataggactc gaaatcgtga
     4381 agcaagatgg tagagtgggc cgtcgagagt gtcaacgcag gacttgacga cgacctcctc
     4441 gacagggacc gatgacgacc atgtacgacg acactacgag caccggcgac gtgaggccgc
     4501 tcagcatctc cgaactcgag agcctcacgg acacaccgcg cagcaccatc tactactacg
     4561 tccgcgacgg gctgctgccg gcggcgcaga aggcggcggc gagccgcgcc gtgtacagcg
     4621 aggtccacgt cgaggctctt cgcgagatcc gcaccctcaa aggggatggg gtgccgctcg
     4681 acgagatccg ggagcgcatc cgcccgctcg tcgagcgtcg tcaggctgcc gagcccgacc
     4741 tcgtggcgcg acgcacgcag cagacccgtg aggccatcct gcaggccgcc gcgctgcagt
     4801 tcgcccgccg cggctaccgg cgcacccgcg tcggcgacat catcaaggag gtcggcatca
     4861 cgccgccggt cttctacgcc cacttcacca ccaaacggca gttgttcatc gaggcgttca
     4921 acgtgttcgt gcactggatg ggcgacctga tcgagccgcc gctcgccgac gagcccgacc
     4981 ctgcggtccg cctgctgatg aggttctacg cgtactgggg tctccagcga ctgagccccg
     5041 atctgctcgg cctggcgcgc gcggaggcgc tgcaggagga cggcgagacg cgcgccgccg
     5101 tgcaggaagc gcagcgctgg atcaccggcg gaccggcccg cgacctcgcc ggcctgcgcc
     5161 gcggcccgga cgacccgccc gtctccgacg agctcatggc ctacagcctg tacggcgccg
     5221 gcgaggagat cttcatgcgg gcctcgtggg acgacgccta ctcgccgcgc gagatcctcc
     5281 gcgcgcacct gttcctgtac ctggccgtcg acgccgccta caccggccgg tgcgacacgg
     5341 tggacaggct gaggagctac gagccgctga tcgaccgtct ggtcgagcaa ggaccgccga
     5401 taccgcagcc gcgagacgcg tccatctaga gccgctcgcg tcacgccggc tccttcgcgc
     5461 gccgctcgcg gcccggcagg ttccacccgc tgtacttctg cgcgtcgatg cgcgcctcca
     5521 gactggcggg gtcgagcacc gccgcaacag ggctgctcgc ctcaccgagc gtgtagtcgt
     5581 agaccacgcg ctcggtcgtg accatcgaga agctcccgga gggatccacc ggcgcgtcct
     5641 cggataggac ggctgcgcgc acgccgctgt cctgcgtgaa cgcgagcagg gcatcgaggt
     5701 cctcgaacca cagttcctcg atgcccgcgt agccgccgac gccggcgagc gcgaacagcg
     5761 tgccctcgaa ggtcttcttg tcgacctcgg cgtggaggtt ctgcacgagg cggccaggac
     5821 gcacgccgcc tgccgtgaga agcgccgcgg cgtgcccgtc cttccaggcc gcggcgaact
     5881 cgctctcgct caggtccggg cggctcttga ccacgtggat cagtttgacg cctcccggcc
     5941 ggaagccggg cgcctcgtcg cgcagcgtct ccgtgaggta ctcgatcacg aagctcgaat
     6001 ccccgaagtc gtgcgggtgc atgcgctgca tgtagtcgtc cgagcggaag ggcacgaggt
     6061 tgtcctcggg cctctccagc cagtggtcgg cgaagttgtc ccaggccatg tcggagaacg
     6121 ggtacagcag gcggcttccg tcgagaccgt tcacggtgaa gtgctgcgcg tagcgcttga
     6181 acgtgcgcat caccgacggc gtcgtgatca tgagagcgac gtggatggtc ccccactcgt
     6241 agaagtactt ctcctgcgag tcacccggct tgcgccgcga gatgagctgg aacttgatca
     6301 cgtgacgacc tcggccctca gggctgcagc gggtagtcgg cgaccggcac gtcctcgtac
     6361 gagagcgtca tctcgccgcc ggcatccttg agttcgatcc acttgaggaa gctcgcgtcg
     6421 tcacgctcgg ggaagtcctc gcgcaggtgc cagccacgag actcctcgcg cgccagcgag
     6481 gcgcgataga acatctcggc gccgagcacc atggactcgg cgacgtggca ggcggccagg
     6541 tagtgcgggt cggccgccgc gagcgtcggc acctgctcct tgagcgccag caccttgccg
     6601 agcgcctcgt tcatgcgctc ggcgtgcttg tagatgctgt agccgaccgg ggccatgacg
     6661 tcctgcaccg cgcgcaccat ctcgggggcc gccatgccgc tgcggcccag cggcgcgaag
     6721 acccgcgcct tgagcgcctc cgcctgggcg gcgtccggac gaccggaggc ggcccgggcc
     6781 tcggccaccg ccaccggcgc gcagcggaca ccggagaagg tggcgaagcc gaggcccgag
     6841 ccgcgcatgc ggccgggggg agccggcacg gcgccggcga ggcaagttcc tgcgtaacac
     6901 gtgtcgccga tggcgaacaa cccaggcacg gtggttgcca tctcgtggtc gaccttgacc
     6961 ggggcgagct cggcgttgag gccgggcatg accggctgca tcgggccgtg gaccgtagag
     7021 gcggccatgg ccttcccgat ggtcaagccc cagaacttca tcgagaccgg ccggaccgcg
     7081 agcgcgtcgg cgtggaaggc cggcacgatc tcgttgttca cgtagttctc gatcatgttg
     7141 gcgacgatcg gaccgttgcc ggccttcatc tcgcgccacc actccacgac ctccttggag
     7201 tggacgtccg actcgagctc cggccgtacc cgcttggaga tgttctcgcc cttggcgttg
     7261 tacatgcagt cctcagcgcc ctggcagact tctttggtgt cggcgaagac ccagttgatg
     7321 aagttgccga actcggcgcc gcgcatcgcc gctcccgccc tgtaggcggc ggcgatgccc
     7381 tcgccgccgg cgctcgacca gcgcggcatg agcttgtagc actggttgcc gttggcgagc
     7441 acgacggcgc cggcctcggc gatcacgcag ccgccgtcga tcatgccgaa gccgatcgcg
     7501 ccgcagaccc tgtcgccgtc cttgagcagg tcgacgatct ccacgcggtc ctggatgcgc
     7561 acgcccagct tgcgcgcgtg ccgagccatg ttgagcgtga ggtcctgatc gagcaccgcc
     7621 aggcgccagg gatgctgcgg cagccagcgt acgtacctgg gctcgccgtc gtcgtcacgg
     7681 tagatgtgca cgccccagct ctcgagcttc tcgaggctgc cgcgtgtctc ccgggcgaag
     7741 tccatgagga ggtcctggtc ctcgaggaag tcgcccacgt tctgcacgtg gaagtcgagg
     7801 acattgccca gctccgccgg gtcgacgaac acgatgttgc cgccgccctt gttggccttg
     7861 ccgccccagc cggtgacgac cttgtcgacg atgagcacgt cgacctcggg gtcggcctcc
     7921 ttggcgctga tggcgcaggc gaggccggcg atgccgccgc cgacgatgag gaggtcggcc
     7981 ttcaggacgg tgccgaggtt ctcgagggag gtcatgtcac accacctccg gccagtagat
     8041 gccggggaag tcgacgacca ccttgatgca ctcggccggg cactcgagct cgcacttgtt
     8101 gcagccgacg cagtcctcgg cgtaggcgac caccggccgg tcgctctcct cgtcccagcg
     8161 gtacacgtcg gcgaagcagg cctcgtagca ggtctggcac gcggtgcacg cgtcgcggtc
     8221 aatctcgatg cggttcattc gctcccctct ctcggaggca gggatgattc cagggaagag
     8281 atgtcgggag actggatggc ggcaagctcg tcgacgagcg cggcgtactt ctcctcgaat
     8341 gccgccacgt cgatgctccc gtcgtagagc ttgcggacgg cgatgttcaa caggatattg
     8401 gtccacatga cgtcgctgcg ggagtagcgg tcatcccagg aggcgcgcag gcgcatcgtc
     8461 tggaacgccc cgtacagcgc gtagttcacg agctcgtcgg agaaaggcgg gtcgtctggg
     8521 ctctgccgca gggccgccag ctcctccatc tggccactca gcacgtgctc gtgcgcgcgg
     8581 cgcaactccc gggtcgtctc cgggtcgtcg taggcggcct cgcgtgagag gtagagcagg
     8641 tcggggttga gcccctgcag ggcgtagtcg cccaccatgc gccacaggag gtggaccgcc
     8701 aggtcatcct cgccgtcgag tcgcggctcg atgatgctca tcgagatgcc cagagactcg
     8761 cggtacgcgg cggcgaagag gtcacgcttg gtcgggaagt ggctgtacag gacctgcggg
     8821 gtgataccga gctcggcgat gatgtcggcg atgcgcgtct gccggtagcc gttgcgcgcg
     8881 aactcccgcg cggcggtgcg caggatcgcc cggcgcgtct gctcgccctg ccgggcgacg
     8941 aggtcctcgc cgctctggct cgcggagtgc acgcggtgcc ggacctgctc acggatggcc
     9001 ttgatgtcca gcccatcctg cttcaggcgc cggatctcat cgagcagttc gacatggtcg
     9061 tcggtgtaga gagcgcgcgt tggactcgac ttctgggccg gcggcaggag acccgcgcgc
     9121 acgtagaagt agatcgtgct gcgggacaca cccgactcgg tctcgagctc cccgatggtc
     9181 agcgctcgca tccgcccccc tgtaccagcg gcatgactag actgtattac tttgcagctt
     9241 tagttggcgt gtccgtaact atagccgggg cgcggtgaaa tgcaagggtc gcccgtccac
     9301 gacccggaca ggtgggcgct gtgcggagtg aagcggtcgc tcagagctcg gcgtacctgg
     9361 ccttccggcc caccccatcg accttgaacg agtggtacgg cgcgatctcc gggtcggtga
     9421 tgacgcggta gagccggacc aggtcgggca ggaagacctc ccagttcccg acctcctcgt
     9481 tggcgtgcgc cgcccagatg agaccatcgt cggcggcgtt ctcccttgcc tccacggccg
     9541 aggacatccc gacgcagccg ttgtcgctct tctgccactg atgcacgcgg tagccgagca
     9601 ccggatcgtc acgctgcacg tcgtcgccga ggatgctgat ggcgccgacg atcaccacgt
     9661 ggtcgtactt gatgagatcc ctgatctcgt ccgccacatc ctcgagcttc tccatgcgga
     9721 tgtacggccg gatctttacg ccgtcgctcc aggtctgtgg cgcgatgtgg acgctgccga
     9781 gcggcccgcc cgcctcctcg aggctgatcc accactcgaa gcccttgtgc tggttggggt
     9841 gccagagata gtcggccttc tccatgttgc accagaacca gttcagctgg tacgacgtga
     9901 cgtcgttcag ctcccagtcg atgtgcgtcc aggggttgtc gtcctgccag gtcttgctct
     9961 tcatcacggt gtcctctcat gccacgcttc gtggcttcgc ttgcggagcg gcatcccggc
    10021 ctcaggccgg cacggccacc tcgagcccga gctggaaggc ggcgaggttc acgtccacgg
    10081 tcttcggcgg caccgacttg cggatgaaac tctccagcat ccccggggtg aagggcaagg
    10141 cgctggccat cgcaccgagc atcacgacgt tggccgcttt gacggtcccc gcccgtgcgg
    10201 cgagctcgct ggcgtcgacc ctgcgcgcat ccggccacgc cgtggcgatg cgctcgtcga
    10261 tgccctccgg gtactccacg gcgcccgacg agaccgtgct cgggttcacg cgggcggcgt
    10321 tgtagaccag caggccgccc ggcttgagcc agtgcacgta gcgcagcgcc tcgacctgct
    10381 cgaacgagag caggacgtcg gcggtccccg gcgtgatgag cgcggagtgg accttgtcgc
    10441 cgaagcgcag gtgactggtg acgcagccgc cgcgctgggc catgccgtgg acctcactct
    10501 gcttgacgtc gtagccctcg gcgagggcga cgcggctgag cacgtagctc gcgagcacca
    10561 ctccctggcc gccgacgccc gagatgagga tgttgaagat gggccgctcg gccatcacgc
    10621 gtcctttccg tcgtcggtga gcgggggcct gatcgcctcg taccggcaga gttgcgagca
    10681 cacgccgcag ccgctgcaca cggcagggtc gatctcgacg cgcggctctc cgtcagcgtt
    10741 cctgtacagg ctcagggccg tgcacccggc gcgcaggcac accttgcagc cggtgcagag
    10801 atccgcgtcc acgcggtacg ccggccgctt gatccggtac tcgagcacgc acggcgagac
    10861 ggcgatcacg accgatggct cgtcggaggc gatctcctcc ttgagcgccg cctgcgtctc
    10921 cgcgacgttc agcgggtcga tctcgcgaac gcgggtgatg cccagcgagc gcacgagcgc
    10981 ggggatgtcg acgaggcggg cgtcttgtcc ctgcagggtc ttgcccatgc tcgggttctc
    11041 ctggccccct gtcatggccg tggttcggtt gtcgaggatc acggtgacga tgttgctgcg
    11101 gttataggcg acgtccagaa gggacgtgat gccggagtgg aagaaggtgc tgtcgccgat
    11161 caccgcgacg gggcggttct tcgtgtcggc gttctcgtcc aggactctcg ccatgccgtg
    11221 cgccatgctg atgccggcgc ccatgcaggt actgcagtgc atggcgttga acggcgccag
    11281 cgccccgagg ctgtagcagc cgatgtcgcc gctgacgaac gcgcgcaggc ggcgcagcgc
    11341 gacgaagacg ccgcgatgcg agcagccggg gcagagcgtc ggcggcctgt cgggcaagtc
    11401 ctcgtcggcc gccggcacgt ccagcagcag gctctcgtcc acgccgggga ccccggccgc
    11461 ggagagcgtc ctggccacga ggccgggggt gaactcgccg cacagcggca ggatgtcctt
    11521 gccggcgtcg accgtcaccc cctgcaggcg gatgttctcc tcgaggaacg ggtcgagctc
    11581 ctccaccacg tacagcttgt cgacctgggc ccggaagccg gcgatcagcc gcctcggcag
    11641 gggccagctc atgcccagct tgaggaagct cgcctgcggg aacgcctcct tggcgtagcc
    11701 gtaggccgcg cccgaggcga cgatgccggc gcgcgcgtcg cccatctcga cccggttgag
    11761 cgccgtggtc tcggcgaact cggcgaggtc gagcaggcgc tgctcgacgg ccgcgtggcg
    11821 agggatggcg ttgtgcggca tcatcagctg ctgcgacggg tctcgctcca gctcgagcag
    11881 cggcccggcg ggagtcgccc gctcgccgaa cttcgccacg ccctgcgcgt gcgatgtgcg
    11941 ggtggtcgtc cggaacagca ccggcgtgcc gaagcgctcg ctcatctcgt aggccgcctt
    12001 gatgaactgc aacgcctcgt cggcgtccga cggctcgacc atcgccagct tcgcgaactt
    12061 cgcgtaggtg cgattgtcct gctcgtcctg tgagctgtgc atggcggggt cgtcggcgga
    12121 cacgatgacg aggccgcccg ccatgcggat catcgacgac gagaagaacg ggtcggcgcc
    12181 gacgttgaga cccacgtgct tcatcgtggc gagcgcccgc gcgccggcga gagcggcgcc
    12241 ggtggccgcc tcgatcgcga ccttctcgtt gggcgcccac tcggcgtaga cgtcctcgta
    12301 cctggcgagc gcctccatga tctcggtgct gggcgtgccg gggtacgcgg aggcgaagcg
    12361 cacgccggcc tcccaggcgc cgcgggcgat ggcctcgttg ccggacagga tcacgagctc
    12421 ggaggcgcgg ggctcggcct cggccgcggc cacatcgggt ggggtcatgc gtcgtccttc
    12481 ggtcgggagt ggtgtgagtg cctggaccgc catgcgccgt accgcgccat ctgctcgagc
    12541 accagcgccg ccgcccgtgg agtggggacg acgacgggcg cgtagcgatg gtcgcccgtg
    12601 tacacggcgc gacggaccgg aaggtcgggg acgttgacga tcggcttgcc ggtctccgcc
    12661 atgagcgcgg cgacgccgtc gaggtagcgc cgttcccacg ggctgaagtc ctcgtagtcg
    12721 cccgtggcgg cctgcccacg gacctcgtcg ccggcgttgg ggacgccgag cacggagagg
    12781 gcgacgacgg catccacctc cggacagcgg gcgaccgctg cgagcacgcg cgaggcgacg
    12841 tcgcccccgg ccatggcgac catgtcgaac gggttccggc ggctccagaa cggcggcagg
    12901 atctcatcga tctccgcaag gagctccgac ggcgggtcga cgagcgtcag ctgccgtcgc
    12961 gccatctcgt cggcggcgag gacgcctgcg ccgccgccgt tggtgacgat ggcgatgcgg
    13021 tggccgctcg gcagcgggag gttcgcgagg gccgtcgcca ggtcgagcgc ctcctggacg
    13081 gaggtgcagg tgacgaggcc ggcctggcga gccgcagcct cgtagacggc cgcggagccc
    13141 gccatggcgc cggtgtgcga ggcggcggcc ttgccgccgc tgtcggtcag cccgccgcgc
    13201 agcaccacca cgggcttctt gaggctcgtc gcggtggcga cgtcgaagag acggcggccg
    13261 tcgtcgacgc cctcgagata cgcgaggatg cacgaggtac cctcgtcgtc cgcgaggtac
    13321 tcgaccacgt cgacggcggt cacctgcgcc tcgttgccga caccgatgaa gcagcgcacg
    13381 ccgaggtcgc ggcggtcggc gagcgcggag agctggacgc ccagattgcc ggactgcgac
    13441 acgatggcga cgttgcccgg ccgcgggtgc agcgcgatga atcccgtcgc gtgcaggtgg
    13501 gtcgcgttgg cgatgaggcc catgcagttg gggccgatca gcgtgacgcc ggcctcggcc
    13561 gccgtctgcg cgagcgcctg ttcggcctgt gcgccttcgt cgccggtctc cgagaagccg
    13621 gcggcgatcg ccacggccgc gcggacgccg gccttaccgc actgtgcgat cacctcgggc
    13681 agctggcgcg cgccgaccgc gaggaccgcg aggtccggcg gggcgggcac gtcctggagg
    13741 ctcggcgaca cgggcaggcc gaagaactct ccgcccttgg ggttgactgg gtggatcgct
    13801 ccctcgaacc cgccggcgag gatgttctgc agcgccgacc cgccccagcg ggcgacgtcg
    13861 ccggacgcgc cgacgatggc caccgatctc ggcgccagga cggcctcgag gtccggggtg
    13921 aaggcgcggg aggccggcgc ggccggggtc gcggcggaca ggaccatgag ggcgtcggcc
    13981 gcgaccggct gggcgccgcg gaccagcagc gggttgacgt cgatctcgga gatctgcggg
    14041 tggtcggcgg cgatccgcgc gaccgcctcg atgacggcga cgagccctgc gcggtccacg
    14101 gccggctggc cccggaacgg gccgagcagc gccctgccgt tgatgaggtc ggggagtccc
    14161 gcgacatcgc ccgcggcgcc gggggtgacg gcgagggcga tgtcgcggaa cacctcggtc
    14221 atggtgccgc cgaggccgaa cgcgacgacg gggccgaacg cggcatcgcg cttcatgccg
    14281 accatgaact cgcggctgcc ggacaccatc tcctcgacca gcacggcctc gagggcgtcg
    14341 ccggcgcgct tcgccagcgc ggcgtacgtc tcggccgcct cgtccggcgt gcgcacgtcc
    14401 agcacgacca gctgcttctc ggtcttgtgc tggatcagcg cgccggtcgc cttcatggcg
    14461 acggggactc ccagagacga cgccatgccg gcggcctccg tcgcagaatg cacgaggccg
    14521 ccggccggga tgggcacgcc gtaggcggcc aggacctgct tggcggcggc ctcgtcgagc
    14581 gcgggacggc cggccgcgag cgccgtttcg acgagctcgc gcgcttgcgc gagcgcgtcg
    14641 ctgacctctg gcggcatcac ggcctcctct cctcagtgct cggaaagaca gtaattgcat
    14701 agatggtagc catttagcta ttattgtcaa gactgctata gtccagccaa catcggccag
    14761 ggaacgccat ggtccaccga gggttcgacg acatgtctct cacgccgggc acggcgagcg
    14821 agagcgcgga gcggtacgta cgcgccctca tcttctgggg cgagctgggg ccgggcgacc
    14881 ggctgccacc ggccacggag ctcgccgcgc ggctgggcat ctcccgggtc acgctgcgca
    14941 tcgccctgcg ttccctcgag agcacgggct acctgatcac cacgcgcggc gcgcacggcg
    15001 gctcccgggt gagcgacctc cagacgctgc agaagtgctg ggaccagtgg atgggggagc
    15061 accgcgacga gctcgacgac atcttcgagt tccgcttcac cgtcgagacg cggctggccg
    15121 agcttgcggc ccggcgccgc acggccgacg agctcgcggc catggagcgc gccctggccg
    15181 gggagcacga caaccgccgc cgggcttcac tcttccaggc ggacgccgac atccaccgca
    15241 gcatcgccgc gggagcccac agcccgcgac tggcgctcgc catgaacgcg gtccgcgccg
    15301 acctcttcct gcccttcgac gaagaactgc tggacagcac ggaggacgac gtcttcgcga
    15361 cgcacgaaga catcgtcacg gcgatccgcg aacaggaccc cgagcgcgcg tcggccgccg
    15421 ccacgaagca cgtgcagcgc gtccgcgagc tcgtcgcgcg cgcgctcgag gcgtcgggca
    15481 tcggcggcgg ccggcactga cgaccgatcg cgcctcgggc gcgggagacg gccccggggc
    15541 tacgacttgc cgtacgtcgg cacggtgccg taggcctcgg tgtggaacac ctcctcctgc
    15601 agctcctcat ccctggcctt gtagaccttg cagagcagac acgtgaggtc cggggagccg
    15661 tacatcgggt cgaggtggtc gttgctcgtg agcacgttgg ggttggagat gaacacgccg
    15721 tggtacccct tggccggttc ctcgggatag aaccagttgg cggtgcacat gatcatgtcc
    15781 ttcttgatgc ccgggaaggc gcgcagcttc tggcggatgc ggccccgcgg cgactcgatc
    15841 cagacgatgt cgccgtcctc gacgccgtac ttctcgcagg tctccggatg catctggcac
    15901 tgagggaact ccatgtagct ccgcagccag gggatgttgc ggtactggcc gaggaagtag
    15961 aagggctggc ggaacccgct gctcatgacg agcgggtact cctcgtagag ctccggcgtg
    16021 gagaccgggc tctctcccgg ctcacggaac accggcaacg ggtcgtaccc gagcgactcg
    16081 agatggttcg agtggaactc gaacttcctg ctcggcgtcg ggaagccgcc gccctcgtgc
    16141 cagtagtccg tcttgtactt gtagtacacc tggtcgggcc cgtagcagcc gctgaccagc
    16201 tgctcgctga agtccttcca ggtcatggtg ccgttcttgc ccttgcgcac ctggtaatcg
    16261 agcagtgcct cgacgtcctc gaaccacttg tcggggacga cccgcttgcc gatctcgttg
    16321 aagatccacg cgtcggcctt cgcctcgccc ggggggtcga tgaccttgtt gtgcgcctcg
    16381 atcatgaacc gcgggtgcat gtcgtacacg tcgtcgagct cgagccagtg ggcggccggc
    16441 agcacgatgt cggccgcctc ggccatcggc cccatgaaca ggtcggcgtg cgacatgaac
    16501 tcgaccttct cgatggccga cacgacttcg ttggtgttgg ccaggtgcac gatctgctgc
    16561 ccgccgacgc tgaaccacac tttgatgggg ctcttgccgg cgttgatggc cttgaccatc
    16621 gtgtccgggt tgcaggtgcg ggcggcgccc atcttgtacg tgtcgccgcc gatgatcccg
    16681 gccttcatct cgtcggtgat cggcagctcg aggaagaagt cctcgatcag gccggtctcg
    16741 gggagcaccc acgtgatcat gctgcccggg cgctcgatgt tgccggtgag acccatgagg
    16801 caggtgatgg cgcgcagcgt gtggccgcag ttggcctggc gctcgagaga gcttcccacc
    16861 tggatgcagc ccggcgtgtc gacggcccac atctcggccg ccgtcgtcac gtgctccttg
    16921 gggatccagc agatgtcgga gaccttctcc ggggtgaagt ccctgacgcg ctcgcggagc
    16981 tcgtcgaacc cgaaggtcca gtcctcgacg aactccctgt cccagagccc ctgctcgatg
    17041 atgtggcgca tcatgcccat cgccagggcg cagtcggtgc cgacccgcgg ctggatccag
    17101 agcgaggccc gcgaggcgta cgccgtggct cgcgggtcga tgatgatgag gttcttgcag
    17161 tagtcgagcg agttcatgaa ccagtaggcc atctcggagt cggcgctgct gatctccagc
    17221 tgcttggccc acatgatctg cgtcttgggg aactccccgc cccagccgtg gtaatcacag
    17281 tagagacggc cgtagcccgt gaccaggccg tacaggccga gccgggggct gtggcacacg
    17341 tagccggggg tgatggcgtt gcccgtgccg agcgagcgcg ccatcttcat cgtgtagcgg
    17401 ttgtagccgc ggccggtccc ctgcgagatg gcgatggtgt gcgggccggg atcggcgatc
    17461 gcggccttca tcttgccggc gatggtgtcg agcgcctcgt cccatgtgat gcgctcccac
    17521 ttgccgtcac cttttctgcc cacctgcttc atcgggtact tgatgcggta cgggttgtcg
    17581 atgtgctgga tgctggagag ccccttggag cacatcgtgc ccttggtgag cgagtccggg
    17641 ttgccctgga tgtggacgat cgcgccgtcc tggacagtga cgatcacgcc gcagccgccg
    17701 tggcagctct tgcacgtcgt cacgatctgc ttgcggtcag ccattgtgtc cctttcagcc
    17761 gcgccagcgt gcggccgctt catcccactc ccggaaggac ggatctgtag atgccgacct
    17821 tcaggacgga gtacttgagc gcgaaggcgc cgatcgtgtg gcagacgatc gccacgatga
    17881 gcaggggctc ggtgtcgccc tccatgaacg ccgtggcgac ggagatgacc agcggggtgg
    17941 cgatgccgag gatcaccagc aggacccaga agacccaggc cgtgctgccc ttgatgaggt
    18001 cctgcgcggc gagctccgcg gccgagtgcc gctgataggc gcgggccaga tagccggcga
    18061 tcagcagggc gttgaccacg agcaggatgc ggctccagaa ctcgaggtcg ctgatggcgg
    18121 cctcgtccgc acccgtgatc aggttgacgc ccatcgtcag cgccagaccg tcggcgacgc
    18181 ccatgatgac gaacacgatg ggcagcagcc ccgtgttcca gaacggcagg ctcttgcagt
    18241 agctcatggc gaagccgcag tagacgccgg tgagcaggat gaacacgccg gcgacgatcc
    18301 cgacgatcca ggagagcacc gtcagggcgt ccgaggcaga ggcgctgagg tcgccccagc
    18361 gcaggaagta ccagaagacg atctgcagga acccggcgcc ggtgaagacg atcgagaaga
    18421 acatgccgcg cgccagccag gacgtcctcc aggctttggt gaacggcggg atggcgcgcc
    18481 agaagcggag tggccgcccc aggtacgcga ggtggaacgg gagcttgaag agcacgatga
    18541 ggacccagcc gagcacggtc gcccaccagg cccaggtggt gatctcactg atcccggcga
    18601 gcaggccgac gaggtacgtg cccatgccga gcgcggagaa gacctcggcg agccacacga
    18661 ggatgccctt gccggagatc cactccttct ggcgcatggg cttgaccatc cactcgtgcg
    18721 tggtgatcca ctggcggcgg gtctgcgcct tcacggcttc gcctccgcat ccgccggctc
    18781 tctgccgtag atgcgccgga aggcgtcgcg gtccacggag ctgaggtgct ggatgtgact
    18841 gcccgtctgg gcctcacgcg gcgccttgtt gtcgttgccg ccgagcctgt agtcgatgta
    18901 ccagacggaa ggctcggtgc cgtactcggc gcgcagctgg aacccgccgt acctgcggat
    18961 gagccggttc acctcgctgt ccgggtcgtc gaggtcgccg aacgtgagcg ccctggcttg
    19021 acacgtgttg acgcaggccg gcgacgcgtc gcggtcgacg cccggcgtga ggccgcggga
    19081 catcccatcg tcgatgcgct cgacgcagaa gttgcacttc tccgtcgtgc cccgctgatg
    19141 cgggtagagc ttctggccca tcttctcgaa ccgcgtgcgc tcgtacccgg ggaagtagcc
    19201 cggatccttg tccttggaat agaaggtgcg gttctggtac gggcaggcga tgacgcagta
    19261 ccggcagccg atgcacttgt ccgagtcgat gacgacgatg ccgtcctcac gctggtgaga
    19321 ggcctcggtg gggcaggcgt gcacgcaggg ggcgtcggcg cactggttgc agcgcaccgg
    19381 gtacgtggac acctcgaccg cgccgtcctc atccacctcg tgggcgatga gcttgaccca
    19441 cgtgatgtcc aggggaagga agtgctcgat cctgcaggcg gcgacgcagg cgtggcagcg
    19501 catgcacttc gccagattga tgaccattcc ccatctcatg ggcggactcc tgtcatcggg
    19561 gactggactt ggccggccgg cggcgggagt gtcaccgagg gccggatgtg ttccgttgta
    19621 tggaacgggc gggccggtcc gcggaccggc ccgcccgaat cctatctcac cgcgccctca
    19681 gctcgacagg gcgatgtctc cgaggttgac gtcctcggcg gcgtcgaccg cctcgtatgc
    19741 cttcgcggcg aagccgtccg cctcgatgct cagggcatag gtaccggcca cgatgtccgt
    19801 gaaccagaag tcgccgaact cgtccgtgga gacgtgcagg accgcgccgt cggccgccgt
    19861 gagcgtgcac gtcgcgccga tgacgacctc cttctcgacc gggtcgtaga ccgtgccggc
    19921 gatgaacttc ttcgggaggt tcaggtagtg gacgcgcggg ctggtgccgg cctccggctt
    19981 catgacctcg gcccgcgcga tcaggccggc gagctcgctc tcctcggcga acgtgatggc
    20041 cttggtcggg cacgagtcga cgcagcgggg gtactcccac tcgtcgtcgt cgagcaggtg
    20101 ggcgcagccg gtgcacttct gggccaggtt gaggtcgtcg ctgaagtaga tcgacccgta
    20161 aggacacgcg gacacgcacg ccttgcagcc ggtgcacttg tcggtgtcga tcacgaccat
    20221 gccgtcgtcg cgcttgcaga tggcgccctc gacctggcac gcctccatgc aggcggcggc
    20281 gtcacagtgc atgcaggggt tgggcacgta gtgcatgcgc accttgggca cggtgccgcg
    20341 gatgaactcg tccacgcgca tccagaactg cccggcgtcg ggctggggct tggcgatggg
    20401 ggcccagtcg ttgccgacgt gctcgtcctt gcagacgaac tggcagccgt agcagccgtt
    20461 gcagcgggcg acgtcgatga cgaatgcttt catcgttacc tgctccctcc ctcgaccacc
    20521 cagccgtcga agcgcagtcc ggccccgggg tcgtattctc gctcgaaggc ctcggggtag
    20581 tcccgacgcc acgtctccca ctcctccggc ctgatcttct gcacgtcgac gagatagccg
    20641 ctggtggcct ggccgcagca ctggcgggag gtgaggccgt tcggggcgat ggtgttgatg
    20701 gcgccgccgc ggtcgacctt gcccgggatg atggggtcgt gacgggcgcc gtggtcgatg
    20761 taggcgacgc ccggcatcag gcgctcccac acgcaggcgc cggcgagcac gatgccgcgc
    20821 tcgttgaaga tcttgacgat ctcaccgtgc tcgatgccgc gctccgccgc gtccgtgggg
    20881 tgcagccaca cgggttcgta gttgtagttg tcgggaccga ggaccttgcc cgtcggcgtc
    20941 tcgcgcgccc aggtgcagtc gtcgccctgg gcgtgcgtgc gccagcgcgg gtggttgctc
    21001 atgagcacga gcgggaagag gtcggcgcgc tcgctcgaga tgcgctcgtc atgcgtgacg
    21061 ccgcgctcga cccacttggg gatcggcggc cgctcctcgt cgtgcgggaa gtgcgtggcg
    21121 aggcgctccg agtagaactc gagcagaccc gagggggtct tgagcggcga cgccttgggg
    21181 tcgtcgtaga actcgcgcag accgggcggg tcgctctccc agtcctccgc cgtgggatac
    21241 acgacgtact tcttctcttc gaactcctcc caggtcatgt acctggagcc gtacatgcac
    21301 tcgaagacct ccttctggag atcccgcaca ctcttgccct gggtgatctt ctccttgagg
    21361 ccgagcttgt cggcgacggc gaggacgatc tcgtagtcgc tcctggactc gccgaccggc
    21421 tcgatggcct tgtcctgcag ggtgcacgtc tggaaggggg gaccctggcg gacgttggtg
    21481 acgcagtcgt cgacctccat cgtggtgttg gccggcagga tgatgtcggc gtagaggcag
    21541 tcgttctcca gccacgggtg ctgcgcgacc atgaactcga tgctctcgtg ccggtgcgac
    21601 tcgatgatcc agttgccgtg gttccagcag gtgatgcggc agggggtgtc cgtccagatc
    21661 atgtgcagct cgctgccgcc ctcttccttg ggcagcggga actcgtactc gatgaactgg
    21721 tcctcggtct ccgcgttctg ggcgccggtg ccggtgaact tgagcggccc gtgcaggatc
    21781 gcctcgtgga tcagcgtctt gggcaggttc tgggtcgccc aggcgtcgac gctgctctgg
    21841 acgggcacgc gcaagtgctc gctgagcgtg ggattgaagt agcggaagtt gtcgagagac
    21901 tcggcgcgag gcatgccgaa gtacgacatc tgccactggt gcacgccggg cccgccgagg
    21961 ccctgcatgc cgagcaggat gcactcgagg cggcccggct cgtgcgaata cgggccgcgg
    22021 atcatcgagc cgccgaagta gtgcacgatc gacgtgcact tggccgcgaa ctcgcgggcg
    22081 agcgccttga tggtccactc gggcacgccg cacttgggcg cggcccattc gggcgacttc
    22141 gggatgccgt cttcgtcgcc gagcacgtag gccgcgacct tgtccatgcc gaccgcgtgg
    22201 gtcgcgacgt actccttgtc ccaggaatct tccttgagcc acgtatagat gatcgcgagc
    22261 tgcagggcgg cgtcgctgtt ggggaggacc gggatccact tgtcggcgtg gacggccgcg
    22321 ccgtagttga ggtcggggca gatgtagacc tgcttgatgc ccgcctcggt ccagaagtag
    22381 gagatgttgg tggcgttctg gccggtgaag ccccacggcg tggtctcgtc gtcgcagccc
    22441 cacttgagga ccatgtcgca gtgctcggag atgtccttgc cgacattgag ctgcggggcc
    22501 atcatgccct ggaacccgtg gccccagacg tgcttggagc cccagtacca gccctcccag
    22561 ctgtccggct ggcggacctg cagggtgaag ccgcccatcg cgtcgagcag gcggccgctg
    22621 tgcccgtgcg gcgtgtggat ggtcttgctc tcgccgtgac cgtcaccctg gaccaggatg
    22681 gcgttgggac cgtactgctt ctggatgcgg acgatctcct tggcgatgat gtcggtggcc
    22741 tcatcccacg agatgcgctc gtacttgctc acgccgcggt tctgcgggtt gcgctcgccg
    22801 tcggggtccc agtcgactct cttgagcgga tacaggatgc ggttggggga gtaggtgcgc
    22861 ttcttgtagg cggtcgcata tggcgcgggg cgcgacttga ggccgggttc gaggacggca
    22921 ccgttgcgct cgatcttcca gggcctgaag gtggagccgt cgtacttcca gtcgaagtgg
    22981 aacgggcgga tgcgcaggat cttgccgtcc ttcacgtcga ccatgacctc gccggtgcca
    23041 ccgaggagac cgccctgcga caaggccttg atgacggtct tgtcgggctt cacgtcgact
    23101 ttcttgcgtg cgtcctcact catctcaatc gctgtccttt cgccttggtt gacgctcccg
    23161 tggctggatc gggctgtgac tcgagagacg gccgggacgg attccagccg agataggcgg
    23221 acaacgaggc cgccgcatcc ttgacggcac ggacgcagag atctcgctct ccggggccgt
    23281 agcggcccgc gggcatgacg acggagatcg cggcgaccac gttgccgagc tggtcgcgga
    23341 cgggcgagcc gacggagcag acgctgagat acaacccctc gtcgtcgaat gcgacgtcgt
    23401 ccgcggcgac gcgctcgagc tcacgctcga gcgcgccggg atcggtgacg gtgcgcgggg
    23461 tgtgccggcg tcgttgctgc gccagaacgg cggcgcggcg ctccggtggc gcgaacgcgg
    23521 tgaagatctt gccgtgcacc gacgcgagat cgccgatgat gcgccccggg gcgatccggc
    23581 gctggaacgg cctggcggaa ttcaggatgc tgacgcagac cgggacgccg tccacgggga
    23641 ccgcgagcgt gacggactca cccgtctcgt ccaggagccg cacgaggtac ggcctcgcgt
    23701 gctccacgaa ctccgaggtg tcctcggcta cgtaggccat cgtgatggcg gcgggcccga
    23761 ggtgatactg gttggtcgcg tggtcgcgca cgaggtactg ggcggcttcg agcgtgcgca
    23821 ccatgcgata ggccgtcatg cgcagcaggc ccgtgcgcgc cgcgatctcg tcgatcgtcc
    23881 actcacggtg gtcgacgtca tacagggaga ggatggagag gccgcgcgcg agcgcgcgca
    23941 cggtgagcgg gtcgacgtcc cccgagcgct ctcccctgcc gctggcctga ccgtccgtgg
    24001 cgcgagccga cgtggtcgtc atgctgtccc cttcatcttg gccccttgcc tgccgccctc
    24061 ccgcgggagt acgatggtcc agccgtatcc ggtatttgcg ttatcaccag ccgatattcg
    24121 catattcacg agatgtacga gtgtggtcca aatcgcctcc ggatgcaagt gattccttgc
    24181 gagtcaccac gggagctggt ggggaggtcg ccgcatgagt gagcaggtct tcaccaacct
    24241 gacgaactgc gggcccgtgt ccgtgtacgt caatgacggg gtggtcacgc ggatccgtcc
    24301 gctggtggcc gacgaggccg agttccggcc ttgggtgatc gaagccggcg gcaagagcta
    24361 ctcgccgccg aagaagttca atctcgctcc gatggtgcac gccgagcgcc ggcgtctcta
    24421 ctcggacgac cgcatcaagt acccgatgaa gcgcgtcgac ttcgaccccg acggcgagcg
    24481 caacccgcag aatcgcggca agtcggccta tgagcggatc tcctgggacg aggcgctcga
    24541 catcgtctcg agcgagatcg cgcgcgtgaa gaagaagtac ggcagctcgg cgatcagcgg
    24601 catgacgtcg tcgcaccaca actggggcat cgtcggctac aagatgggcc cgttcatgcg
    24661 cttcatgaac tgcctcgagg ccacccccgt cttcgacaac ccggacagct gggaggggtg
    24721 gcactggggc gccacgcaca cgtacggctt ctactggcgc ctcggcatgc ccgagccgtt
    24781 cgacaacctg caggacgtca tgcgcaactc cgagatgatc gtcttctggt cgaacgaccc
    24841 cgacacgacg cgcgggacgt actcgggaca ggagtcggcg atctggcgga cgtggctcaa
    24901 ggagaagggc gtccaggcgg tcttcatcga tcccttccac aactacacca acgcgatctt
    24961 cgccgacgcc aagtggatcg cgccgcgcat gggcacggac acggcgctgg cgatggcgat
    25021 cgcccacgtg tggatcaccg aggacaccta cgacaagcag tacgtcgccg accgcaccat
    25081 cggcttcgac gagttcaagc cctacgtgct cggcgagacc gacggcgtgc ccaagacgcc
    25141 ggagtgggcg gccgaggaga gcggcgtcgc ggcgcgcgtc atccgcacgc tggcgcggga
    25201 gtgggccgcc aggcccaccg ccctcgcggc cggcgcccgc ggcggcgaag ggagcgcctg
    25261 ccgtaccgcc tacgccacgg agtgggcgcg gatgatggtg ctcctccagg cgatgcaggg
    25321 gctgggcaag ccgggcgtca cgatcggcgg cgtctctatg ggcgccccgg cagagttcga
    25381 cacctggttc ccggcctacg ccgagccgat gggccgcatg tcccactcga cgaaggcggc
    25441 cgcgtacatc ccgcagaact cgaccaagca gcgtctctac cgcctcaccg tgccggacgc
    25501 catcctcgac ggccacatcg agtgggacgg cgcagggttc tgcggccagt cgctcgagca
    25561 gcagttcgag cacttcgtgt acccggcgga gggctgctcg gagatcaagc tctggtaccg
    25621 ctacggcggg tccttcatgg gcaccatgag cgacaccacg aagtgggtgc gcatgtacca
    25681 gagccccaag ctcgagttcg tcgtcaacca ggacatctgg ttcaacagcg agacccgcat
    25741 ggccgacgtc atcctgccgg cgtgcaccaa cttcgagcgt gacgacctcg gcgagttcgc
    25801 cgcctgcaac ggctacacca cgaactcgca ctcgggcaac aactaccggg tcatcgtgcg
    25861 tgagcagaag tgcatcgagc ccct
//
LOCUS       DASUMX010000036        15487 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1017124, whole genome shotgun sequence.
ACCESSION   DASUMX010000036 DASUMX010000000
VERSION     DASUMX010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 15487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15487
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1017124"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..1100)
                     /gene="gap"
                     /locus_tag="VFZ86_01570"
     CDS             complement(105..1100)
                     /gene="gap"
                     /locus_tag="VFZ86_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009307178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEX5641007.1"
                     /translation="MTVRVGINGFGRIGRLVIRAAQRHGADVEFVGINDLTDSKTLGH
                     LLKYDSVHGLYPGTVAVEEDALVIDGKRVRVTAESDPAKLPWRELGADVVIESTGRFT
                     ERAKAAAHLEAGASKVIISAPGKGADATIVLGVNEETYDKTTHDVISNASCTTNCLAP
                     MAKVLVDNFGVEKGFMTTIHAYTGDQKVLDFPHRDLRRARAAALSIIPTTTGAARAVS
                     LVIPELEGKLDGFALRVPTPDGSATDLVAELKTEVTAEEVNAAMKAAAEGPMQGILEY
                     QEDPIVSIDIVGNSHSSIFDPALTMAKGKLVKVISWYDNEWGYSCRLVDLVQRVL"
     gene            complement(1254..5423)
                     /locus_tag="VFZ86_01575"
     CDS             complement(1254..5423)
                     /locus_tag="VFZ86_01575"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEX5641008.1"
                     /translation="MKTAPVPFDSEALRANLATTAQEVVIPERYLPLLEAVDGLHGVR
                     AALADTMGEYFHTFRNADLLVEGFQTTLLRNWSYFERAPNRAELFGLLAELVVGLLES
                     PLTGDQFSLLLRPLLTWATDVLTGPNGDQYDEPLAGLAGALARVLPAREAEFLERDTL
                     LRNLAQRAQERPSLAPGYHALFRALLAAGYRRVRDSLDVPAWALSQGEHLTDPGAVAG
                     KFAFLTGRRIGTLLDALAAAPDEKLLTPNFPVFSTILAEAIDSLFRVENLEDRFAVCL
                     FFLKDETLGYRQKEVMADLLGVVKQMMRPDRHTDAVRILARLTEFFRDRDNEFLLTRF
                     ACYEAIGVAIGEAGNVRAADHLIEDILYWKFQYPDIHGATDEWETVVNPYHLPKVRCW
                     MRIIESNPALFERLAAALNVQLKLGGVFIADTDLFQRDVTRFLDADIRPIYFVAKQLL
                     RTLPVYFNEVGAEGELRAVSTQIDEICGRRDTLMHFLRKQVHAESSNRMVPFSREVLR
                     YWLTLDPSGLEPYVSANTLAAVRAERQWAEGPHEVLVALAADAGRAADEGLLEELAAL
                     PPAVLEERLAPDGDDGRAPSAVARRRVALLVRTHQLLARKYSLAAADVGSAVSHHLNL
                     DGRTRHRFEVALARWQARPGRRTRDALLDAALTVLERLKAVILAPAAVAATENIYQKR
                     HIAAGIPSIYGNYSEPRFDALGLSFRVETLIGRLLDDVVEEGVERYVTRASLRRMATA
                     LRRFERALAVDGVDSRALDANLDMLEASFSLRNFTFRQYQNVFQFLSNSVTELSSTSV
                     LSHEQVLHAVLVNDPRQCEARGLSVDAVAELVLREVLVSGLGLQALDRYLAMALRQIS
                     MLNGRLDGRGLTRMMNYDPERLVSPIHKPKRGTDDQTTLGFKGLGLKQMAAYGHRVPE
                     GFVLSTELFGAVPAMSYPPLYDDTIRRVQEAIHVLERRAGLRLGDPRRLLLLSIRSGA
                     AISMPGLMTTFVNVGLNDELVEAFARRPETAWAAWDSYRRFLQSWAMSYGIDRDFFDT
                     IMTEYKARYGVGQKLDFTAGQMREIAFAYKSRARDHGVVFVDEPFGQVVACMRKVLES
                     WDSAPARVYRQYIGVAEEWGTAVVMQRMVFGNLSRESGSGVTFTNNPLEPHSRQVRLF
                     GDFAVRSQGEDLVGGLVFPWPVSEAQRLGSLTYRGTEHSLERDFPDIYQQLLAVARDL
                     VGEREFDPQEIEFTFESPAASDLYILQKRSAVQEQTRDAAYFDTTSPNYGPPVAVGMG
                     VAGGAYSGRAAVTAEQIDRLLAEAPDENIVLLRPDTVPEDIGMITRVSGILTARGGAT
                     SHAAVTAKRLGKTAVVECVDLEVFEKAGTVRLASREVDVGDWVSIDGRTGNIFFGRIP
                     TTPQPPLPPAR"
     gene            5547..6782
                     /locus_tag="VFZ86_01580"
     CDS             5547..6782
                     /locus_tag="VFZ86_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HEX5641009.1"
                     /translation="MAAQRTLGINGIGRIGKLTLWHHLGRDDFDRFVVNTGRPVGTSL
                     DSVVQYLSKDSTYGPLHRFLFGRSGTRDMSVLDEERGLISAYGKEILILREARNPKDI
                     PWRDNGVALVVDATGTFRDPHADADAPRGALRGHLAAGAAVVVQSSPFKSRQKGLPLP
                     QDAAILVHGINDYEFDPARHTLVSAASCTTTALAHMIRPLLDRDLTKDMITAGMSTIH
                     AVTNSQPVLDSVPKAGATDLRKTRGAIGNLVLTSTNAAAALEQVMPEIGTIGFMGDSV
                     RVPTQSVSLIILNVTFQSETLADGSVSVERSAINAIYEDAAAGEANGLLKYTDEQNVS
                     ADLLGEDAAVVIEGAETHTRTGFMNLRVPPCEPGGAARPDTVRVPLTHVKVFGWYDNE
                     MGSYTYRLGELTIRAARSM"
     gene            complement(6796..7722)
                     /gene="whiA"
                     /locus_tag="VFZ86_01585"
     CDS             complement(6796..7722)
                     /gene="whiA"
                     /locus_tag="VFZ86_01585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEX5641010.1"
                     /translation="MGFTRDVKLELVTVMPPAVHCRSAQLSGLLFGAGVFEISAGGHF
                     GVRVAVTLPAVARHVLALLKPLRVDASLRTVDSAPIGLRYEILLGDEARDLQVLNELG
                     VLSDEFAVQMTVPRRLVERHCCLVAFLRGLFLGCGSLSGPGAPVHAEFTVEDESFAEQ
                     VQRLLARLELPFSLAIRERNVACYTKRGQTAADLLAVLGAHDSCLRWEEHAVLGTVRE
                     SANRLANCDAANARRAAAAGRRQAALLRALLDSPEGDDLPAPLREVAELRIEYPYLSL
                     TELAAAADPPLSRSAVNHRLRRLVALAVEAAS"
     gene            complement(7726..8976)
                     /locus_tag="VFZ86_01590"
     CDS             complement(7726..8976)
                     /locus_tag="VFZ86_01590"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEX5641011.1"
                     /translation="MRRSLEWLAPGLGLKRWVLLAFAGGVLLVYSVIFGIGVLWSPQL
                     MRGLGMGWLENRHWAALLLSVDGVAVGLFALLYGARRVWLFQRTTPESVSEIRAAARL
                     ARGARVVAVGGGNGLAALLRGLKSQTSNLTAVVTMADDGGSSGLLRRDMGMPPPGDLR
                     NCLVALADDESMMSRLFQYRFPEGGGLQGHSFGNLFMAALSEVTGDFEHAVRESTRVL
                     KVRGRVLPSTLEDVVLHAQLEDGGHVSGESTITAAERLPRRVWLTPDAPRGLPQAFAA
                     IARADLVVLGPGSLYTSVIPNVLVPEVRQAIADTRAWVVYVCNVMTEPGETDGYTAAD
                     HVAALHRHGLVGMIDAVLVNDAPVSEEMAASYERFGARPVPVDDERLKALGVKVIRAP
                     VAAESGVVRHDSERLSRALLRLVR"
     gene            complement(8990..9859)
                     /gene="rapZ"
                     /locus_tag="VFZ86_01595"
     CDS             complement(8990..9859)
                     /gene="rapZ"
                     /locus_tag="VFZ86_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEX5641012.1"
                     /translation="MTEALQFVIITGLSGAGKSQAMDSFEDAGWFCVDNLPPELVPTL
                     VDLFRREGSKVDRVAVVSDVRGGEYFGRLEQLLDELHERGVEYTLVFLEASNEALVRR
                     FKETRRRHPLAGGGSVIDGIGRERDRLAGLRERAHLIIDTSDISIHQLKARLNEDLIR
                     HTRRTNIVFAVVSFGYKYGIPIDADLLFDVRFLPNPHYDLRLRPLTGMDEEVSSFVLE
                     SPGTSEYLERLYPLLDFLFPRYAAEGKAHLTIGVGCTGGRHRSVTIAQVIGQRYEKQG
                     YYTVVRHRDLTRD"
     gene            complement(9856..10389)
                     /locus_tag="VFZ86_01600"
     CDS             complement(9856..10389)
                     /locus_tag="VFZ86_01600"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEX5641013.1"
                     /translation="MRTSIRPDEVAGLGRLTEEAFVCALGVGYVLLAGGLPIALELPR
                     AAASLRPGRRGTSHWTAELERYFAGERVTFTLDVDAYAEVHGFTDFERDVYRALARVP
                     YGRVASYRDLATAAGRPNAYRAVGSAMARNALPVILPCHRVIKNDGRLGSYGDDPAWK
                     ERLLALEGVRVVEGRVT"
     gene            complement(10364..12226)
                     /gene="uvrC"
                     /locus_tag="VFZ86_01605"
     CDS             complement(10364..12226)
                     /gene="uvrC"
                     /locus_tag="VFZ86_01605"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEX5641014.1"
                     /translation="MTPAARQQSGLGRLRAVLKRIPNEPGVYRFLDAGGAVLYVGKAK
                     ALRKRVANYLRPSAQPHGRTGEMLAQAADIDWVVTASESEALLLEDNFIKEERPPYNL
                     RLRDDKSYPSIEVTLRDEWPRVRFFRGRRVPGNLYFGPFSSARKVRETLELIGRIFPY
                     RKCRGAEPGRPSGSPCLQYFIKRSLGPCDERCTREEYLATVHQAIAFLRGRLGEVEKD
                     IAREMAAAAAAQEFEKAALLRDRLAAVRHVHERQSARVDEREALDVVGLSLAEPGANV
                     QMFLVRDGAVVDRQAFYLENAAGREPAEVLEEFLLEYYWEGTVIPPEVVAPLEDGAVL
                     GAVLGERRGSGVSVRAAKRGPKRRLLELAQHNAELAAQAEVERIAQKSASRIEALERL
                     RDGLALERLPLRIECYDISNLGEKHPVGSMVVFEGGRPKKAHYRKFAVRDAAGQDDFA
                     MMNQVLRRRFERRSAEAGDYDESFATLPDLVVVDGGKGQLSAAVAALADTGVDVDVVS
                     LAKQREEVYVPGRRDPLPLPATDPGSLLLQRVRDEAHRFAVTYHRQRRDADLRRSSVF
                     DELPQVGPVRRRRILEHFGSPERFLSASREEIERVPGLPRKVAREIYAHLHKTG"
     gene            12326..13207
                     /locus_tag="VFZ86_01610"
     CDS             12326..13207
                     /locus_tag="VFZ86_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020508448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEX5641015.1"
                     /translation="MTSSSLPPQKQMTGMLGHPVAENPIDLMFDAVYAHYGLPWTFWK
                     SDVAAADLGAAIAGTRALGFRGYGITVPYKIDAIPYLDEVDDDARTIGAANYVTVEDG
                     RLTGHNNDGKGVVKAITKVTPIAGKRVAMLGAGGAGRAMAVELGWAGAAHLTVVTRRE
                     SQGREVAELVHRAAGIPTAWQRWEGRVTVPDGTGLLMNATQLGSAPGMEPVPIVWDSL
                     DPSATAVDVITNPRATPFLTTAKERGCAVVDGVEMLVQLAMQLFESWTGVSPEEQVFQ
                     RAVSEALGEEKPRRPGA"
     gene            complement(13240..14016)
                     /locus_tag="VFZ86_01615"
     CDS             complement(13240..14016)
                     /locus_tag="VFZ86_01615"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5641016.1"
                     /translation="MNGITAFAYDTFWLGLRTSRRFIRVPANLISIIFFPLIQLLVFS
                     QLFKDIIQLPAFATDSYLGYLAPGQVVFASFLAVGWAGYGMLVEYRTGYLDKLRATPI
                     ARTSILAGEMVPLFFEAAFIGGVILGVSVLLGATLVTGVGGLLLILFLSGVFGIAFAG
                     LNFIAALLTKSEQAVSALSLLMFPVVFMSTAFVPVELMPGWMQSFNAWNPVTFQIEAV
                     RTLMTEGYDWSAIGSALLADAIVGVVLLAVTLWAFRRLAD"
     gene            complement(14019..15029)
                     /locus_tag="VFZ86_01620"
     CDS             complement(14019..15029)
                     /locus_tag="VFZ86_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018141771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5641017.1"
                     /translation="MASSAGTVIEVRDLVKIYGGETRAVDQVDFEVDRGELFGFLGPN
                     GAGKTTTIRILATLLTPTSGAARVAGFDVVEDSAEVRRRLGLALQTPTLDAFSTGRET
                     LELAGRLHRMPRSDIRGRTDELLQLMGLEAAAKKLTGTYSGGMKRRLDLAAALMHRPE
                     VLILDEPTEGLDPQSRTALWEELERINAEGTTMLLTTHYMEEADRLCTRLAIIDNGHV
                     VVEGRPEALKAGVGADTVTLQVGQDGDGAGDDARRAEFCRLLEGMVPCEAVAAHPEGV
                     TLSVADASAAIPGLLRRLDGNGLDIAGLRMSRPSLDDVFLKYTGRHIREEAADQPIIL
                     GF"
     gene            complement(15178..>15487)
                     /locus_tag="VFZ86_01625"
     CDS             complement(15178..>15487)
                     /locus_tag="VFZ86_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019885357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HEX5641018.1"
                     /translation="LHFQDIDKLLEMLQRLVDQGNTVVVIEHNLDVIKSADWVVDLGP
                     EGGDEGGLVVATGTPEELAGLPDVSHTGEFLARALAGAAAERQPAAPAEKPARKARVG
                     "
BASE COUNT         2303 a         5420 c         5596 g         2168 t
ORIGIN      
        1 acgttgaagt ccacgcgcac gagcgcgcgc ttgcccgcga gcgggtagtc ggtgagcgac
       61 cgcttcatgg cgtcttcagc ctccccggcg tgacgtgacg agtatcagag gaccctctgc
      121 acgaggtcga cgaggcggca cgagtagccc cactcgttgt cgtaccagct gatgaccttg
      181 accagcttgc ccttggccat ggtgagcgcc gggtcgaaga tggacgagtg gctgttgccg
      241 acgatgtcga tggacacgat ggggtcctcc tggtactcca ggatgccctg catcggaccc
      301 tcggcggcgg ccttcatggc ggcgttgacc tcttccgcgg tgacctcggt cttgagctcg
      361 gcgacgaggt cggtggccga gccgtccggc gtcggcacgc gcagggcgaa gccgtcgagc
      421 ttgccctcga gctccgggat gacgagcgag acggcgcgcg ctgcgccggt ggtcgtcggg
      481 atgatgctga gcgcggcggc gcgggcgcgg cgcaggtccc tgtgcgggaa gtcgagcacc
      541 ttctggtcgc cggtgtaggc gtggatggtg gtcatgaaac ccttctccac gccgaagttg
      601 tccaccagca ccttggccat cggcgccagg cagttggtgg tgcagctggc gttgctgatc
      661 acgtcgtggg tggtcttgtc gtaggtctcc tcgttgacgc cgaggacgat ggtggcgtcg
      721 gcgcccttgc ccggcgcgga gatgatcacc ttgctggcgc cggcctcgag gtgagcggcg
      781 gccttggcgc gctcggtgaa gcgccccgtg gactcgatga ccacgtcggc gccgagctcg
      841 cgccagggca gcttggccgg gtcggactcg gcggtgacgc ggacgcgctt gccgtcgatg
      901 acgagcgcgt cctcctcgac cgcgaccgtg cccgggtaca ggccgtgcac cgagtcgtac
      961 ttgagcaggt ggccgagggt cttggagtcg gtcaggtcgt tgatgccgac gaactcgacg
     1021 tcggcgccgt gccgctgcgc ggcgcggatg acgaggcggc cgatgcggcc gaagccgttg
     1081 atgcctacac gcacggtcat gaagtcctcc cgatcgtttt gacggacagc ttaccagagg
     1141 cgactgtccg atacaggcgt gaattgttca cttttgcgcg actagtgcgg cgtgcgcatg
     1201 aagtggctga aggctcggcc ttccggcgcc gtggactcct cgccacccgc ccctcagcgg
     1261 gctggtggaa gcggcggctg cggcgtcgtg gggatgcgcc cgaagaagat gttgcccgtc
     1321 cggccgtcga tggacaccca gtcgccgacg tcgacctccc ggctggcgag acgcacggtc
     1381 ccggccttct cgaagacctc gaggtcgacg cactcgacca cggccgtctt gcccaggcgc
     1441 ttcgcggtca cggcggcgtg cgaggtcgcg ccgccgcggg cggtgaggat gccgctcacg
     1501 cgggtgatca tgccgatgtc ctcgggcacc gtgtccgggc gcaggaggac gatgttctcg
     1561 tccggagcct ccgccagcag ccggtcgatc tgctccgcgg tgacggcggc gcggccggag
     1621 taggcgccgc cggccacccc catgccgacc gcgaccggcg gcccgtagtt gggcgacgtg
     1681 gtgtcgaagt aggcggcatc gcgcgtctgc tcctgcacgg cggagcgctt ctgcaggatg
     1741 tagaggtccg acgccgccgg cgactcgaag gtgaactcga tctcctgagg gtcgaactcg
     1801 cgctcgccga cgaggtcccg ggcgacggcc aggagctgct ggtagatgtc ggggaagtcg
     1861 cgctccagcg agtgctccgt cccccggtac gtgaggctgc cgagacgctg tgcctcggag
     1921 acgggccacg ggaagacgag gccgcccacg aggtcctccc cctggctgcg cacggcgaag
     1981 tccccgaaca ggcggacctg gcggctgtgc ggctcgagcg ggttgttggt gaaggtgacg
     2041 cccgagcccg actcgcggct gaggttgccg aagaccatgc gctgcatcac gacggcggtg
     2101 ccccactcct cggcgacgcc gatgtactgg cggtagaccc gcgccggagc cgagtcccac
     2161 gactcgagca ccttgcgcat gcaggcgacc acctggccga aaggctcgtc gacgaacacg
     2221 acgccgtggt cgcgggcgcg ggacttgtag gcgaaggcga tctcgcgcat ctgccccgcg
     2281 gtgaagtcga gcttctgccc cacgccgtac cgcgccttgt actcggtcat gatggtgtcg
     2341 aagaagtcgc ggtcgatgcc gtaggacatc gcccaggact ggaggaagcg gcggtagctg
     2401 tcccacgcgg cccaggccgt ctccggccgc cgcgcgaacg cctcgacgag ctcgtcgttg
     2461 agccccacat tgacgaacgt cgtcatgagg ccgggcatgg agatcgccgc tccggacctg
     2521 atggacagca gcagcagccg gcgcgggtct cccagccgca ggcccgcccg gcgctcgagc
     2581 acgtggatgg cctcctggac gcgacggatg gtgtcgtcgt agaggggcgg atacgacatc
     2641 gccggcaccg cgccgaagag ctcggtgctc agcacgaagc cctcggggac cctgtggccg
     2701 taggcggcca tctgcttgag gccgagcccc ttgaacccca gggtcgtctg gtcgtcggtg
     2761 ccgcgcttgg gcttgtggat cggcgagacc aggcgctccg ggtcgtagtt catcatgcgg
     2821 gtgaggccgc ggccgtcgag ccggccgttg agcatggaga tctggcggag cgccatcgcc
     2881 aggtagcggt cgagcgcctg caggccgagg cccgagacga ggacctcgcg caggacgagc
     2941 tcggccaccg cgtccaccga caggccgcgc gcctcgcact ggcgcgggtc gttgaccagc
     3001 acggcgtgca gcacctgctc atggctgagc acggaggtgg acgagagctc cgtcacgctg
     3061 ttggacagga actggaacac gttctggtac tggcggaagg tgaagttgcg cagtgagaag
     3121 ctcgcctcga gcatgtccag gttggcgtcg agcgcgcgcg agtcgacgcc gtcgacggcg
     3181 agcgcgcgct cgaagcgccg cagggccgtc gccatgcggc gcagggaggc gcgcgtgacg
     3241 tagcgttcga cgccctcctc caccacgtcg tcgagcagcc ggccgatgag ggtctccacg
     3301 cggaaggaga gccccagggc gtcgaagcgc ggctcgctgt agttgccgta gatcgatggg
     3361 atgccggcgg cgatgtgccg cttctggtag atgttctcgg tcgcggccac ggccgccggc
     3421 gcgaggatga cggccttgag acgctcgagc accgtgagcg cggcgtcgag cagcgcgtcc
     3481 ctggtgcgcc ggcctggacg agcctgccag cgcgccagcg cgacctcgaa gcggtgacgc
     3541 gtgcggccgt ccaggttgag gtggtgcgag acggccgagc ccacgtccgc ggcggccagt
     3601 gagtacttgc gcgcgagcag ctggtgggtg cgcaccagca gggcgacgcg gcgccgggcc
     3661 acggccgacg gcgcacggcc gtcgtcgccg tccggcgcca ggcgctcctc gagaacggcg
     3721 ggagggagcg ccgcgagctc ctcgagcagg ccctcgtccg cggcgcgtcc ggcatcggcg
     3781 gcaagcgcga ccagcacctc gtgcgggcct tccgcccact gccgctccgc gcggaccgcc
     3841 gcgagcgtgt tggccgacac gtacggctcg agacccgagg gatcgagggt gagccagtag
     3901 cgcagcacct cgcggctgaa cgggaccatg cggttggagg actccgcgtg gacctgcttg
     3961 cgcaggaagt gcatgagcgt gtcgcgccgc ccgcagatct cgtcgatctg cgtcgagacg
     4021 gcgcgcagct cgccctccgc ccccacctcg ttgaagtaga cgggcagtgt gcgcagcagt
     4081 tgcttggcga cgaagtagat gggtcggatg tccgcgtcga ggaagcgcgt gacgtcgcgc
     4141 tggaagagat cggtgtcggc gatgaacacg ccgcccagct tgagctgcac gttgagggcg
     4201 gcggccagcc gctcgaagag cgccgggttg gactcgatga tgcgcatcca gcagcggacc
     4261 ttgggaagat ggtagggatt gaccaccgtc tcccactcgt cggtggcgcc gtggatgtcc
     4321 gggtactgga acttccagta caggatgtcc tcgatgaggt ggtcggccgc cctgacgttg
     4381 ccggcctcgc cgatggcgac tccgatcgcc tcgtagcagg cgaagcgcgt gagcaggaac
     4441 tcgttgtcgc ggtcgcggaa gaactctgtg aggcgcgcga ggatgcggac ggcgtcggtg
     4501 tgcctgtccg gtcgcatcat ctgcttgacc acgccgagga ggtcggccat gacctccttc
     4561 tgccggtagc ccagcgtctc gtccttgagg aagaagaggc agacggcgaa ccggtcctcc
     4621 aggttctcca cccggaacag ggagtcgatg gcttccgcca ggatcgtcga gaacaccggg
     4681 aagttgggtg tgagcagctt ctcgtccggc gccgcggcca gcgcgtcgag cagggtcccg
     4741 atccggcggc cggtgaggaa ggcgaacttg ccggcgacgg ccccggggtc ggtgaggtgc
     4801 tcgccctgcg agagcgccca cgccgggacg tcgaggctgt cgcgcacgcg gcggtacccg
     4861 gccgcgagca gcgcccggaa cagcgcgtgg tagccgggcg cgagcgaagg acgctcctgc
     4921 gcccgctgcg cgaggttgcg caggagcgtg tcgcgctcga ggaactcggc ctcgcgcgcc
     4981 ggcagcaccc tggcgagcgc gccggcgagc cccgcgagcg gctcgtcgta ctggtccccg
     5041 ttgggcccgg tgagcacgtc cgtggcccag gtgagcagcg gccggaggag gagcgagaac
     5101 tggtcgccgg tcagcggcga ctccagaagg cccacgacca gttccgccag cagcccgaac
     5161 agctccgccc tgttcggggc gcgctcgaag tacgaccagt tgcgcagcag tgtggtctgg
     5221 aagccctcca cgagcaggtc ggcgttgcgg aacgtgtgga agtactcgcc catcgtgtcc
     5281 gcgagcgccg cgcgcacgcc gtgcaggccg tccacggcct ccagcagggg caggtagcgc
     5341 tcggggatca ccacctcctg cgccgtggtg gcgaggttgg cgcgaagggc ttcggagtcg
     5401 aacggtacgg gcgcggtctt cacgcaccca tgatacctcg ccgctcgcgg gggttccggg
     5461 taggatgacc gcgacgggac aggcgagcgg cgcggagagg cccgtgcgac ggggaggtcc
     5521 gcgtcgcgac aggaggtacg gcagcgatgg ccgcacagcg caccctcggc atcaacggca
     5581 tcggcaggat cggcaagctg acgctgtggc accacctcgg ccgcgacgac ttcgaccgct
     5641 tcgtcgtcaa caccggccgg ccggtgggca cctccctcga ctccgtcgtg cagtacctct
     5701 ccaaggacag cacgtacggc ccgctccacc gcttcctctt cggccggtca ggcacccgcg
     5761 acatgagcgt gctggacgag gagcgcggcc tcatcagcgc ctacggcaag gagatcctca
     5821 tcctgcgcga ggcgcgcaac cccaaggaca tcccgtggcg cgacaacggt gtggccctgg
     5881 tcgtggacgc caccggcacg ttccgcgacc cgcacgccga cgcggacgcg ccgcgcggcg
     5941 ccctgcgcgg ccacctcgcc gcgggcgccg ccgtggtggt ccagagctcg cccttcaaga
     6001 gcaggcagaa gggactgccg ctcccgcagg acgcggcgat cctcgtccac ggcatcaacg
     6061 actacgagtt cgacccggcg cggcacacgc tcgtgtcggc cgcgtcctgc acgacgaccg
     6121 cgctggcgca catgatacgg ccgctgctcg accgcgacct caccaaggac atgatcaccg
     6181 ccggcatgag caccatccac gcggtgacca actcgcagcc ggtcctcgac tcggtcccca
     6241 aggccggggc cacggacctg cggaagacgc gcggggcgat cggcaacctg gtgctcacgt
     6301 cgaccaacgc cgccgccgcg ctggagcagg tgatgcccga gatcggcacc atcggcttca
     6361 tgggcgattc ggtgcgcgtg cccacgcaga gcgtgagcct catcatcctc aacgtgacct
     6421 tccagagcga gaccctggcc gacggctcgg tgtccgtcga acgcagcgcc atcaacgcca
     6481 tctacgagga tgccgccgcg ggcgaggcca acggactgct caagtacacg gacgagcaga
     6541 acgtctccgc cgacctgctc ggcgaggacg cggcggtggt catcgagggc gcggagacgc
     6601 acacgcgcac ggggttcatg aacctgcgcg tgccgccctg cgagccgggc ggcgcggccc
     6661 ggccggacac cgtccgcgtg ccgctcacgc acgtcaaggt gttcggctgg tacgacaacg
     6721 agatgggcag ctacacgtac cggctcggcg agctcacgat ccgcgccgcg cgcagcatgt
     6781 agccggcggc gccgatcagc tggccgcctc gacggccagg gcgacgagcc ggcgcaggcg
     6841 atggttgacc gccgagcggc tgagcggggg gtcggccgcg gccgccagct ccgtgaggct
     6901 caggtacggg tactcgatgc gcagctccgc gacctcgcgc agcggcgccg gcaggtcgtc
     6961 tccctccggg gagtccagca gcgcacgcag gagggccgcc tggcgcctcc cggccgcggc
     7021 cgcccggcgc gcgttggcgg cgtcgcagtt ggccagccgg ttggcgctct ccctcaccgt
     7081 gccgagcacc gcgtgctcct cccagcgcag acaggagtcg tgcgccccga gcaccgcgag
     7141 caggtcggcg gcggtctgcc cgcgcttggt gtagcaggcc acgttgcgct cgcggatcgc
     7201 cagcgagaag ggcagctcca gccgcgccag caggcgctgg acctgctcgg cgaagctctc
     7261 gtcctccacg gtgaactcgg cgtgcaccgg cgcgcccggc cccgataggg agccgcaacc
     7321 gaggaagagg ccgcgcagga acgcgaccag gcagcagtga cgctccacca ggcgccgcgg
     7381 caccgtcatc tgcacggcga actcgtcgga cagcacgccg agctcgttga gcacctgcag
     7441 gtcgcgtgcc tcgtcgccga gcaggatctc gtagcggagg ccgatcgggg cgctgtccac
     7501 ggtccgcagc gaggcgtcga cccgcagcgg cttgagcagc gcgagcacgt gccgggcgac
     7561 cgccggcagc gtcaccgcga cacgtacgcc gaagtggccg ccggcagaga tctcgaacac
     7621 gccggcgccg aacagcaggc ccgagagctg ggcgctccgg cagtggacgg ccgggggcat
     7681 cacggtgacg agctcgagct tgacgtcgcg ggtgaagccc atcgcctacc gcacgaggcg
     7741 aagaagggcc cgggacaggc gctccgagtc gtgccgcacg accccgctct cggcggcgac
     7801 gggcgcgcgg atgaccttga ccccgagcgc cttgagcctc tcgtcgtcca cgggcaccgg
     7861 acgcgcgccg aagcgctcgt agctggccgc catctcctcg gacacgggcg cgtcgttgac
     7921 gaggacggcg tcgatcatcc ccaccagccc gtggcggtgg agcgcggcca cgtggtccgc
     7981 ggcggtgtac ccgtcggtct cccccggctc ggtcatgacg ttgcagacgt agacgaccca
     8041 cgcgcgcgtg tcggcgatcg cctgacgcac ctcggggacg agcacgttgg ggatcacgct
     8101 ggtgtagagg ctgcccgggc cgagcacgac caggtcggcc cgggcgatcg cggcgaacgc
     8161 ctgcggcagg ccgcgcggcg cgtccggcgt gagccacacg cgccgcggca ggcgctcggc
     8221 ggcggtgatc gtgctctcgc cgctgacgtg gccgccgtcc tcgagctgcg cgtggagcac
     8281 cacgtcctcg agcgtcgagg gcagcacgcg gccgcgcacc ttgagcacgc gcgtggattc
     8341 gcggacggcg tgctcgaagt cgccggtcac ctccgagagc gcggccatga agagattgcc
     8401 gaagctgtgg ccctgcaggc cgccgccctc cgggaagcgg tactggaaga gccggctcat
     8461 catcgactcg tcgtcggcca gcgcgaccag gcagttgcgc aggtcgccgg gcggaggcat
     8521 gcccatgtcc cggcgcagca ggcccgagct cccgccgtcg tcggccatcg tcacgacggc
     8581 ggtcaggttg ctcgtctgcg acttgagccc gcgcagcagg gcggccaggc cgttgccccc
     8641 gccgaccgcc accacgcgcg cgccgcgcgc caggcgggcg gcggcgcgga tctcgctcac
     8701 cgactcgggc gtcgtgcgct ggaagagcca gacgcgacgc gcgccgtaca gcagcgcgaa
     8761 caggccgacg gcgacgccgt ccacgctcag gagcagcgcc gcccagtgcc ggttctccag
     8821 ccaccccatc cccagcccgc gcatgagctg cggcgaccac agcacgccga tgccgaagat
     8881 gaccgagtag accaggagca cgccgccggc gaacgccagc aggacccagc gcttgagccc
     8941 gaggcccggg gcgagccact ccagcgacct gcgcatcagc cggatctgct cagtctcggg
     9001 tgaggtcgcg gtgccggacc accgtgtagt agccctgctt ctcgtaccgc tggccgatca
     9061 cctgggcgat ggtcaccgag cggtgccgcc ccccggtgca ccccacgccg atggtgaggt
     9121 gggccttgcc ctccgccgcg tagcgcggga agaggaagtc gagcagcggg tacaggcgct
     9181 cgaggtactc gctcgtgccg ggcgactcga ggacgaagct gctcacctcc tcgtccatgc
     9241 cggtgagcgg ccgcaagcgc aggtcgtagt gggggttggg caggaagcgg acgtcgaaca
     9301 gcaggtccgc gtcgatcggg atgccgtact tgtagccgaa cgacaccacc gcgaagacga
     9361 tgttggtgcg ccgggtgtgc cggatgaggt cctcgttgag gcgcgccttg agctggtgga
     9421 tggagatgtc gctggtgtcg atgatgaggt gcgcgcgctc gcgcagcccg gcgaggcggt
     9481 cgcgctccct gccgatgccg tcgatgacgc tgccgccgcc cgccagcggg tgccggcggc
     9541 gggtctcctt gaagcggcgg acgagcgcct cgttcgaagc ctcgaggaac accagcgtgt
     9601 actcgacgcc gcgctcgtgc agctcgtcga gcagctgctc gagccggccg aagtactcgc
     9661 cgccgcggac gtcgctgacc acggcgacgc ggtccacctt gctgccctcg cggcggaaca
     9721 ggtcgacgag cgtgggcacg agctcgggcg gcaggttgtc gacgcagaac cagccggcgt
     9781 cttcgaacga gtccatggcc tgggacttcc cggcccccga gaggcccgtg atgatcacga
     9841 actgcagcgc ctcggtcacg tcaccctccc ctccacaacg cgcacgccct cgagcgccag
     9901 caggcgctcc ttccaggccg ggtcgtcgcc gtacgagccg agccggccgt cgttcttgat
     9961 gacccggtga cacggcagga tcaccggcag ggcgttgcgc gccatggcgg agccgacggc
    10021 gcggtaggcg ttgggccggc cggccgcggt cgcgaggtcg cggtagctcg ccacccgccc
    10081 gtagggcacg cgggcgagcg cccggtagac gtcgcgctcg aagtcggtga acccgtggac
    10141 ctcggcgtag gcatcgacgt ccagcgtgaa cgtcacgcgc tcgcccgcga agtagcgctc
    10201 gagctcggcg gtccagtggc tcgtgccccg gcgccccggg cgcaacgacg ccgccgccct
    10261 gggcagctcg agcgcgatcg gcagtccccc cgcgagcagc acgtagccga cacccagcgc
    10321 gcacacgaag gcctcttctg tcagccggcc caggccggcc acctcatccg gtcttatgga
    10381 ggtgcgcata gatctccctc gcgaccttgc gtggcagccc cggcacgcgc tcgatctcct
    10441 cccggctggc gctcaggaac cgctccgggc tgccgaagtg ctcgaggatg cgccgccggc
    10501 gcacggggcc gacctgcggc agctcgtcga agaccgacga gcggcgcagg tcggcgtcgc
    10561 gccgctgcct atggtacgtc accgcgaacc ggtgcgcctc gtcgcggacg cgctgcagca
    10621 gcagcgaccc ggggtcggtg gccggcagcg gcagcgggtc gcggcgcccg gggacgtaca
    10681 cctcctcgcg ctgcttggcg aggctgacca cgtcgacgtc gacgccggtg tccgcgagcg
    10741 ccgccacggc cgcggacagc tgccccttgc ccccgtccac gaccaccaga tcgggcagcg
    10801 tggcgaagct ctcgtcgtag tccccggcct ccgccgagcg ccgctcgaag cgccgccgca
    10861 gcacctggtt catcatcgcg aagtcgtcct ggccggcggc gtcgcgcacc gcgaacttgc
    10921 ggtagtgggc cttcttcggc cggccacctt cgaagaccac catggacccc accgggtgct
    10981 tctcgccgag gttggagatg tcgtaacact cgatgcgcag cggcaggcgc tcgagcgcga
    11041 gcccgtcgcg caggcgctcg agcgcctcga tgcgcgacgc cgacttctgc gcgatgcgct
    11101 cgacttcggc ctgcgcggcg agctcggcgt tgtgctgggc cagctcgagc aggcgccgct
    11161 tgggaccgcg tttggcggcg cgcacgctga ccccggagcc gcgccgctcg ccgaggacgg
    11221 cgccgagcac ggccccgtcc tcgagcggcg ccaccacctc gggcgggatc accgtcccct
    11281 cccagtagta ctcgagcagg aactcctcga gcacctcggc gggctcgcga ccggccgcgt
    11341 tctccagata gaaggcctgc ctgtcgacca ccgccccgtc gcgcaccagg aacatctgca
    11401 cgttggcgcc cggctcggcc agcgagagcc cgacgacgtc gagcgcctcg cgctcgtcca
    11461 cgcgcgccga ctggcgctcg tgcacgtggc gcacggcggc gagcctgtcc ctgagcagcg
    11521 ccgccttctc gaactcctgc gccgccgcgg ccgccgccat ctcgcgcgcg atgtccttct
    11581 ccacctcacc gagccggccg cgcaggaagg cgatggcctg gtggacggtc gccaggtact
    11641 cctcacgcgt acagcgctcg tcgcacgggc cgagcgagcg cttgatgaag tactgcaggc
    11701 agggcgagcc gctggggcgc cccggctcgg cgccgcggca cttgcgatag gggaagatcc
    11761 ggccgatgag ctcgagcgtc tcgcggacct tgcgcgcgct cgagaacggg ccgaagtaca
    11821 ggttgccggg cacgcggcgg ccgcggaaga agcgcacgcg cggccactcg tccctgagcg
    11881 tgacctcgat ggacgggtag ctcttgtcgt cgcgcaggcg gaggttgtac ggcgggcgct
    11941 cctccttgat gaagttgtcc tcgaggagga gcgcctccga ctcggacgcc gtcaccaccc
    12001 agtcgatgtc ggccgcctgc gcgagcatct cgccggtgcg cccgtggggc tgcgccgacg
    12061 ggcgcaggta gttcgccacg cgcttgcgca gcgccttcgc cttgcccacg tagagcaccg
    12121 ccccgccggc gtcgaggaac cggtacacgc cgggttcgtt ggggatgcgc ttgaggacgg
    12181 cacgaaggcg cccgagtccg ctctgctggc gcgccgctgg agtcatcgcc tcgattgtac
    12241 ccgccgcgaa gcgggcgccg gagcgccggg agcgccgttc gggcttcggt acgatgcggg
    12301 cagacagccg accaaggagc cagcgatgac atcctcctcc ctgccgccgc agaagcagat
    12361 gaccgggatg ctcggtcacc ctgtggcgga gaatcccatc gacctcatgt tcgacgccgt
    12421 ctacgcgcac tacggcctgc cgtggacgtt ctggaagagc gacgtcgcgg cggcagacct
    12481 cggcgccgcg atcgcgggca ccagagcgct cggttttcgc ggctacggca tcacggtgcc
    12541 gtacaagatc gacgcgatcc cgtacctgga cgaggtggac gacgacgcgc ggaccatcgg
    12601 ggcggccaac tacgtgaccg tcgaggacgg ccggctcacc ggccacaaca acgacggcaa
    12661 gggcgtcgtc aaggcgatca ccaaggtcac gccgatcgcc ggcaagcgcg tggccatgct
    12721 cggcgccggg ggcgccggcc gggccatggc cgtcgagctg ggctgggccg gcgccgcgca
    12781 cctcacggtc gtcacgcgtc gcgagagcca ggggcgcgag gtcgccgagc tcgtgcacag
    12841 ggccgccggc atcccgaccg cgtggcagcg atgggagggc cgcgtgaccg tcccggacgg
    12901 caccggactc ctcatgaacg ccacgcagct ggggtccgcc cccggaatgg agccggtccc
    12961 catcgtctgg gacagcctcg atccgtcggc cacggcggtc gacgtcatca cgaatccccg
    13021 ggcgacgccg ttcctgacga cggcgaagga gcgcggctgc gccgtcgtcg acggcgtcga
    13081 gatgctggtg cagctcgcca tgcagctgtt cgagtcgtgg accggcgtct cgcccgaaga
    13141 gcaggtgttc cagcgcgccg tgtcggaggc gctgggcgag gagaagccgc ggcggcccgg
    13201 ggcgtgacgc ttcgcccgcc ccgggccgcc ggaacgccct cagtccgcca gccggcggaa
    13261 cgcccagagc gtcaccgcca ggaggacgac cccgacgatg gcgtcggcca gcagggccga
    13321 gccgatggcg ctccagtcgt agccctcggt catcagcgtg cggaccgctt cgatctggaa
    13381 cgtgaccggg ttccaggcgt tgaagctctg catccacccc ggcatcagct cgacgggcac
    13441 gaacgccgtc gacatgaaga ccaccgggaa catcagcagc gagagcgcgc tcaccgcctg
    13501 ctcgctcttg gtgagcagtg cggcgatgaa gttcaggccg gcgaaggcga tgccgaacac
    13561 gcccgagagg aagaggatca gcagcagccc gcccacgccg gtcacgagcg tggcgccgag
    13621 cagcacgctc acacccagga tcacgccgcc gatgaacgcg gcctcgaaga acagcggcac
    13681 catctcaccc gcgaggatgg acgtgcgcgc gatgggcgtg gcgcgcagct tgtcgaggta
    13741 gccggtgcgg tactccacca gcatcccgta ccccgcccag cccacggcga ggaacgatgc
    13801 gaagacgacc tgccccgggg cgaggtagcc gaggtagctg tcggtggcga aggccggcag
    13861 ctggatgatg tccttgaaga gctggctgaa gaccagcagc tggatgagcg ggaagaagat
    13921 gatgctgatc aggttcgcgg gcacgcggat gaagcgccgc gacgtgcgca ggcccagcca
    13981 gaaggtgtcg taggcgaatg cggtgatgcc gttcatcgtc agaaccccag gatgatgggt
    14041 tggtcggccg cctcctcgcg gatgtggcgc ccggtgtact tcaggaacac gtcgtcgaga
    14101 ctcggccggc tcatccgcag gccggcgatg tccagcccgt tgccgtcgag ccgccgcagc
    14161 agcccgggga tcgccgcgct cgcgtcggcg acggacagcg tcaccccttc cggatgagcc
    14221 gccacggcct cgcagggcac catgccctcg agcagccggc agaactcggc cctgcgcgcg
    14281 tcgtcgccgg cgccgtcgcc gtcctggccg acctggaggg tcacggtgtc ggcgccgacc
    14341 cccgccttga gcgcttcggg gcggccctcc accaccacgt ggccgttgtc gatgatcgcg
    14401 agccgcgtgc agaggcggtc cgcctcctcc atgtagtgcg tcgtgagcag catggtggtg
    14461 ccctctgcgt tgatgcgctc gagttcctcc cacagcgccg tgcggctctg cgggtcgagg
    14521 ccctcggtgg gctcatcgag gatgaggacc tcggggcgat gcatgagcgc cgccgcgagg
    14581 tcgagacggc gcttcatccc gcccgagtag gtcccggtca gcttcttcgc ggccgcctcg
    14641 aggcccatca gctggagcag ctcgtcggtg cggccgcgga tgtcgctcct gggcatgcgg
    14701 tgcagccggc ccgcgagctc gagcgtctcg cggccggtcg agaaggcgtc gagggtcggg
    14761 gtctgcagcg cgaggcccag gcgcctgcgc acttcggccg agtcttccac cacgtcgaac
    14821 ccggcgacgc gggccgcgcc ggacgtcggc gtcagcagcg tggcgaggat gcggatcgtc
    14881 gtcgtcttgc cggcgccgtt gggcccgagg aagccgaaca gctcgcccct gtccacctcg
    14941 aagtccacct ggtcgacggc gcgggtctcg ccgccgtaga tcttcacgag gtcacggacc
    15001 tcgatgactg tccctgcact gctggccatg gcgtcactcc tctcctctgc cgtcgccgtc
    15061 gtgctcggtt aacggtcccc ggcgacttcg aagagaaaca caccatcatc ggtcgtggtc
    15121 atcccgcagc ggagcatcgc cgcgggaaca gggccggcgt gcggagcacg cctcccgtca
    15181 cccgacgcgg gccttgcgtg ccggcttctc cgccggagcc gcaggctgcc gctccgcggc
    15241 ggcgccggcc agcgcccgtg cgaggaactc gccggtgtgt gagacatcgg gcagaccggc
    15301 gagctcctcc ggcgtgccgg tcgcgaccac cagcccgccc tcgtcgccgc cctccgggcc
    15361 gaggtcgacg acccagtcgg cgctcttgat gacgtcgagg ttgtgctcga tgacgaccac
    15421 ggtgttgccc tggtcgacca gccgctggag catctcgagc agcttgtcga tgtcctggaa
    15481 gtgcagg
//
LOCUS       DASUMX010000037        18531 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1304707, whole genome shotgun sequence.
ACCESSION   DASUMX010000037 DASUMX010000000
VERSION     DASUMX010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 18531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18531
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1304707"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..826)
                     /locus_tag="VFZ86_01630"
     CDS             complement(<1..826)
                     /locus_tag="VFZ86_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper oxidase"
                     /protein_id="HEX5641019.1"
                     /translation="MKTAFGDSRGGAAGVVATLSLAVLTLVLAITSAPAGAVPLPGGS
                     LDPMSIPKYREPLVIPPAMPRSGVLAEPYGRQIDYYEIVVKQFQQYILPEEWAEENDI
                     DPTTVWSYASTRDPRPVADGGTLNYPALTVEGKTGRPVRVKWINGLVDADGDYLPHLF
                     AVDQTLHWANPAGGPGGTDTHGMDAMPYTGPVPIVTHVHGAHTTEDSDGYPEAWYLPA
                     ANDIPAGYATGGSLFDQFKQEAEARQGQTWEPGAAVFQYPNDQRPTTLWYHDHTLGM"
     gene            1175..2242
                     /locus_tag="VFZ86_01635"
     CDS             1175..2242
                     /locus_tag="VFZ86_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641020.1"
                     /translation="MVDHESIPRTVHTTPPRRRSAAGAALLAVLLLTLAAVAAVTAPH
                     AAAAPSLSALQKEARRAKAEMASLQTDLQAVGRRLAEAQQRLDEVNQRLVRSRIDLRR
                     AENELALQREVLAERAAAMYKQGDLDWLDLLSTIQSFADIDTLRSLQRALADQDRRTE
                     DDAQRLAREARRLERRVESDHEDAVAAEQEVQNQRIELDQRLAERSAILQDVTRRIKK
                     ILASGGLGPALAMARNGQFTQLTWARALLVAMRLPVTADNVAAVAAWEMAEGGHWYNT
                     AYYNPLNTTQSMPGATVFNSVGVKAYTSWRQGLEATVKTLRNGYYGDIIAALRRGNDS
                     TAVAAAVGASPWGTGDFSRLL"
     gene            2323..2514
                     /locus_tag="VFZ86_01640"
     CDS             2323..2514
                     /locus_tag="VFZ86_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641021.1"
                     /translation="MTDRQGRVVGVRTPPPPAPEESRATRVWRRRENLAATGELRHQA
                     ELRNQYAAFHVDPGPSVRR"
     gene            2519..3370
                     /locus_tag="VFZ86_01645"
     CDS             2519..3370
                     /locus_tag="VFZ86_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641022.1"
                     /translation="MSAGSWILLAAVVAVAVLWGVPRLLGRRRDRRRRELIRRLEQAR
                     GTRVITLIHRQETMSLLGLPFGRYIDIDDSEQLLRAIRLTPPEMPIDLLLHTPGGLVL
                     ASDQIAHALKRHDGRVTVIVPHYAMSGGTLVALAADEILMDPDAVLGPVDPQLGSLPR
                     GSWPAASILKALEQDNPNRDDETLILGDVARKAIDQVRATVRELLEGRVEDAEGERLA
                     ALLSEGRWTHDYPIRLEEARELGLPVSDAVPDLVYELMTLYPRRSQRRPSVEFIPTPY
                     RTRGGDG"
     gene            3385..4116
                     /locus_tag="VFZ86_01650"
     CDS             3385..4116
                     /locus_tag="VFZ86_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010479675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HEX5641023.1"
                     /translation="MDDDRGQAYLRETRHRRSRLVGGRMDWAHQPDWFKTYPDAPRVK
                     LPDLALDDGGIFDALARRRSVRAYSAEPLALGELSALLWAAAGVTARQEGFAFRTAPS
                     AGGLYPIEHYVVASDVEGLEQGLYHYDVLGRALERLHAGDLRLPIARAALDQRVAADA
                     PAVFVWTAVLERSRWKYGERFARYVLLDAGHIAENVALAATALGLGTCQIAAFFDEEA
                     ADLIGVDPDREPVVYMSTAGRPAGE"
     gene            complement(4169..5071)
                     /locus_tag="VFZ86_01655"
     CDS             complement(4169..5071)
                     /locus_tag="VFZ86_01655"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5641024.1"
                     /translation="MGAEATSGAGGAAHSCRHASTQFLGAGGVRLLLQAWLPAGPDPM
                     ELPEPSAVIAVVHGYGDHGGRYVRFGEAMAARGWAVYVYDLRGHGQSSGRRGQVKRFD
                     DYLDDTEMYLDEVRRLQSGKPLLLLGHSLGGLICARLAEERPPAVRALILSSPFLQLA
                     EALPPARVAAAKGLAVVWPHKDIGNTLQAEQLSHDKTVVDAYVTDRLVHHVAPARWAA
                     ETLAAQDAAMAAAERIALPLYVLYGKDDQVVDVAFIEALFARAESGDKTIRSYEGLYH
                     ECFNEVGRERVYEDLAAWIAPRVR"
     gene            complement(5076..6197)
                     /locus_tag="VFZ86_01660"
     CDS             complement(5076..6197)
                     /locus_tag="VFZ86_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5641025.1"
                     /translation="MKYRRMPIEEESPEQFGYDRIRYNLTESSVRDRSLSDLGVTLED
                     GLLFYGDHAGNERLRDLIAEQSGGPDGVTGDDVLVTAGAAQALFIVATSLLDRGDHLV
                     VIRPNYATNIETPRAIEADVSFLDLTFEDGFRIDTEKLMALVTPRTRYVSITLPHNPT
                     GVMMGEADLRSLVGEVERAGCRLLVDETYRELTFGEPLPVAATLSERVISVSSLSKTY
                     GVPGIRTGWLVCRDGELMRTFLAAKEQIGICGSVVDEEIAARALEQRAAWLAELHPFI
                     ARQLQVVRHWIAGEELVEWVEPRGGVVCFPRIRPEVPVDVDEYYRVLGEVHGTYVGPG
                     HWFEQDRRHMRIGYAWPLPEELEGGLAAVSASIRAALTS"
     gene            6355..7335
                     /locus_tag="VFZ86_01665"
     CDS             6355..7335
                     /locus_tag="VFZ86_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641026.1"
                     /translation="MARSHRRVSVTIALVAALLLAAAAAAACSARATTGSASLATGAA
                     PTLGAGEDDADGSGAGARGASGSDWSRVLAALAWMQADTPTRPVVVLLGGSAARESTI
                     SDQSWRDQIVAEGGPETLAWNMGSRNRTMAQNVALVKALPRGAEAIVYIGVNLGSFTS
                     AQKTASITLPSPAPASSPSLQQPHQYSTSTGILPTTKKKALVRAWRADRYPVFTRNFR
                     TSAAVLQTLIRVCLARGYRPVLFELPRNTAVIGSSLSAPTARYRDKCRVLAAQYKIPW
                     VSFVKAAGLPNGDFYDLWHLVEPGRTVWQGLLSAKTAALLERYGYDGGGS"
     gene            7319..8407
                     /locus_tag="VFZ86_01670"
     CDS             7319..8407
                     /locus_tag="VFZ86_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641027.1"
                     /translation="MAAARKALSRRPVRVAARLTLILLLAAGLAALTLVAAPARAGDG
                     ASGAPTTALLGAAPAPAPSFAPALVPPVGEEARGTDYGKDWQHCVQVVANLRAAPPTT
                     PLVVLLGGSSARESTVLDDHWERQITRRSGYVVDAYNLGSKHRSYAQDLAFVKLLPAG
                     VPTIVYVGVNLGRFCLPVRPASITLPSPRTLPPWGQHVYSRDRIQTRAQKRYYVSYWL
                     ARRYPEFRANYKSELVMLERIIRACKRRHLRVALVDLPRDLPVIGSAFDAPVSRYHAG
                     CARLARAWDIPWLHFNSQARFADRDFFDIFHLVEPGRVKFQSILSDRTIGLLKRYRMP
                     RPEPTPSPSPSPSPSASTSAPASPAPAF"
     gene            8420..8983
                     /locus_tag="VFZ86_01675"
     CDS             8420..8983
                     /locus_tag="VFZ86_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007523958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HEX5641028.1"
                     /translation="MSTLTLLGIALGLAMDAFAVAIGAGLQLCNVTKRQTFRLAWHFG
                     LFQAFMPILGWLAGLTLVDYIAPVDHWIAFGLLAIIGGKMVYEALEHEEEEADRCDPT
                     KGWRLVMLSIATSIDALAVGLSLAVLGVSIWWPALVIGVVAGALTMVGLELGTRFGAL
                     LGRRMEIVGGLILIGIGVKILVEHLTG"
     gene            8993..10231
                     /locus_tag="VFZ86_01680"
     CDS             8993..10231
                     /locus_tag="VFZ86_01680"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX5641029.1"
                     /translation="MSFPQLPAYRISGPLSAKLRLVTLLAVTAVLWVHANNMSSRFVV
                     GENAAEASGAPGVVGFVEYLVSQALTRWPAAVLFAISGFLFFHNLQPLWSAYRRKYAR
                     RVRTVVVPFLLWSGISLAVYLVLQAVPQSAAYFSSDFFSGLSLGHIADKLLLHPVAYP
                     LWFLQTLIVCFALAPLLYWPARALRWLAPLPFAALWILGAPAANWNDWKGLAFFTLGA
                     VIALERRRGVRLTPAPWVGRWLFPLWVLACVVFTAYLRGESAFWAYSLHKALMCMAVA
                     AVWFGYETYLRPLEGRRLVTFLLPFSFFIFVAQEPLLTTLKRLGLRELGSSDAAMLFV
                     YFAAPLLTLVAVVAVAAALRRYLPTPYAWLTGGRGGRSARRSHGRGRAEDPAPAVAGL
                     EGEPALAETGGAPKPVRRGS"
     gene            complement(10215..10685)
                     /gene="msrA"
                     /locus_tag="VFZ86_01685"
     CDS             complement(10215..10685)
                     /gene="msrA"
                     /locus_tag="VFZ86_01685"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013194952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEX5641030.1"
                     /translation="MATATFAAGCFWGVEQRFAALRGVTSTEVGYTGGSTENPTYEDV
                     CSHGTGHAEAVRLEFDPDLISYQELLAHFFSSHDPTQWNRQGPDVGDQYRSAVFFHDR
                     DQEKAALAAIAQLTESHVFTRPVVTQVVPAGRWWRAEEYHQRYFEKHAGFSCRV"
     gene            complement(10834..12321)
                     /locus_tag="VFZ86_01690"
     CDS             complement(10834..12321)
                     /locus_tag="VFZ86_01690"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX5641031.1"
                     /translation="MKIRLIEPISPSRHLWSKSYFVRLGLPMIGAALVEAGHDVRIFN
                     PQLAPIDWADVYSSDLVGLSSTTSTIPEAYRIADELRRRGIPVIIGGSHVTFMADEAL
                     EHADYVARGEAGEMIMVELAETLAGDRELGSITGISYRGDDGKIVHAPLHERCEDLDT
                     LPFPDLTLVDGHERISNTPIMTSWGCPFDCTFCSVTAMFGKKYRFRSAESVVEEIKQK
                     KPEKIFFYDDNMAANRKRLKRLLQLMIDEGITVPWGAQVRTDVVRDPELMELMRASQC
                     DFVALGLESVNQATLDSFEKSQTVGDIEHAIKVLHDYGIRAHGMFVLGADHDTPDSVS
                     DTVKFAKKNKIDTVMLNILTPLPGTQQFEELDAEGRILERDWSFYDAQHVVFHPKQMT
                     PYHLLQETIKANKRFYATWRPVWAFHKWLWASKKQAAKKQAIWDRMLEYGWLWYYSRT
                     WWTAAKNRAQRRRLRELSRQPQQPPQRQPRPAAPVRAVEPEKQRV"
     gene            12568..12921
                     /locus_tag="VFZ86_01695"
     CDS             12568..12921
                     /locus_tag="VFZ86_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641032.1"
                     /translation="MDVDSRGSLEPLTPGRRSDRIRFYGQGRVCAQPGCTTLLSMYNP
                     SSRCAVHQRRSDVIPLRRLESQEVERGCSQCGARFLTTNPRRKYCSARCRSHAFAERE
                     RAARLSVALRERAAA"
     gene            12966..14102
                     /locus_tag="VFZ86_01700"
     CDS             12966..14102
                     /locus_tag="VFZ86_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HEX5641033.1"
                     /translation="MDAAFFDTPIDRRGTASFKWDMYDGDVLPLWVADMDFASPPPVV
                     EALEAVARRGVLGYALAPDSLAEAVAGHLERRYGWRIDPGWIAWLPGIVPGLNLACDA
                     FAAPGEAILTVTPVYPPFLEAPGHRGRRLITVPAGLRDGRWQLPLDAMEAAVTPDTRV
                     LFFCHPHNPLGRVWRGVDVETVLDFCRRHDLVLVSDEIHCDLVLDDLVHIPSALAGPD
                     DQDRIVTFMSPSKTFNLPGLNLAFAVVADEELRRRLLQPGEGLLPFPGCFAVAAAEAA
                     YRHGGDWHAGLLDYLRGNRDALEAFVAAELPRVRMTHVEATYLAWLDVRALGLEDPAA
                     ACRSAGVALSAGAAFGDPACLRLNFGCRRETLDEALRRLVTVLR"
     gene            14113..15396
                     /locus_tag="VFZ86_01705"
     CDS             14113..15396
                     /locus_tag="VFZ86_01705"
                     /inference="COORDINATES: protein motif:HMM:NF044870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEX5641034.1"
                     /translation="MSPQSRSPRPAGRRPRHAGRARVYRRRRLFALVAVVALVAVLAG
                     VVWAAVGGCGSDPASGGSAASSGGSASGSAEGGEAGLAPSPSPTAWTGPPSSSLKLKL
                     RKTIVDADISPKSVVSSGSGYVFAQNMMYKHTMTVYDSTTLRLVETIPDSIDAARFRI
                     KGHGDTIQGAPVECAFLPDGRFAYVSQYSMYGDGFVEGHDVCSPGGASRSYVYRVSLA
                     DLEVDKVIKVGEVPKYVAVTPDQKYVLVSNWCSYSLSVIDVHTSKVVKDISLGPYPRG
                     IAVSADSKTAYVAVMGTSDIAAVDLEDFSVEWIRGAGSGPRHLCMAGDGKYLYATLNN
                     EGSVAKIDPGTGTVVDKVSTGSQPRSMAIAPDGKSLFVVNYDSSTMSHIRTSDMKVVD
                     TLGTDSLPIGITYDAPTRSVWVCCYSGSIMVFKQV"
     gene            complement(15405..16088)
                     /gene="rpiA"
                     /locus_tag="VFZ86_01710"
     CDS             complement(15405..16088)
                     /gene="rpiA"
                     /locus_tag="VFZ86_01710"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006748007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEX5641035.1"
                     /translation="MTRDEQKRAAAVAAMAYVQEGRAVGVGTGSTAAFFIEELAARPK
                     LVAAAVPSSEASAAALRAAGIRLVGLDETPSLPLYVDGADAVDPRLRLVKGAGGALAR
                     EKVVASAAELFVCIVDESKLVDRLGDPARGAAGVPVPLAVLPMAAAFVAERVRALGGT
                     SAVRRGYRSDDGLAILDVTGLDLGDPERLEAELGVIPGVLESGVFARRAADVVLAGGD
                     DGVRTIVGR"
     gene            16182..16661
                     /locus_tag="VFZ86_01715"
     CDS             16182..16661
                     /locus_tag="VFZ86_01715"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5641036.1"
                     /translation="MKPVNVFTIATETDPEDPPGYRAGAVKLAPRIAAAQIGGSVYDL
                     PPGESVCPYHFEFGAEEWLIVLSGRPTLRRASGQSELEEALEPGDTVCFPPGPSGAHK
                     VTNATEAPVRILMLSTMPEVDVSYYPDSEKFGVWPGGGVKGFLVRRRSEVDYYDGER"
     gene            16678..18030
                     /locus_tag="VFZ86_01720"
     CDS             16678..18030
                     /locus_tag="VFZ86_01720"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5641037.1"
                     /translation="MSMHEPTPEDAAVAHELVDWALARLADRRGVLPARPASGPLPPL
                     APEGIGARAALATLRDAVIPTAIPPDHPRYFAFVPGTATVAATFADMALSAAMIYGGS
                     RLEAGAAVEAEDAVLRWLADAAGFPESAGGTFVSGGSIANLSALVAARGGGEDAHGRR
                     VIVAGASAHSSMAAAAHIMGCELVTAPAADAHGRLDGRVLEMALTGRDRADIVAVVAT
                     AGATNTGAFDDLAGVATICAGHGLWLHVDGAYGGAALLSPRTRPLLDGIEGADSFIVD
                     PHKMLYTPFDCAAVVYRDCTAARRSLTQTADYLDAICDVERGDPSDLAVHLTRRVRGV
                     PLWASVLAYGTGAYAAAVDHCVEVAAYAATRIARTPELELVIAPAFTVLLVRRTGWSA
                     DDYAAWCADALARGVAMLMPTRHAGETVLRFCFVNPLTTREDVDVVLSDLAASSPSPP
                     "
     gene            complement(17993..>18531)
                     /locus_tag="VFZ86_01725"
     CDS             complement(17993..>18531)
                     /locus_tag="VFZ86_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019052855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX5641038.1"
                     /translation="RAADGVAVGDPLDQATFMGPVAGRRPFDTVRDYIDVGAAEGARL
                     VAGGPARPEGLDRGFFVRPTVFADVAPDMRIFREEIFGPVLCMTPFRDEEDAIELANA
                     TDFGLSGYVHSADLERARRVAGRMRTGMVHLNGAPTDARAPFGGYKQSGNGREWGRFG
                     LEDFLEVKAVMGWKPPGR"
BASE COUNT         2537 a         6813 c         6470 g         2711 t
ORIGIN      
        1 tcatgccgag ggtgtggtcg tggtaccaca gcgtcgtcgg ccgctggtcg ttggggtact
       61 ggaagacggc cgcgccgggc tcccaggtct ggccctggcg cgcctcggcc tcctgcttga
      121 actggtcgaa gaggctgcct ccggtggcgt agccggccgg gatgtcgttc gcggccggca
      181 ggtaccaggc ctccgggtac ccgtcgctgt cctcggtggt gtgcgcgccg tggacgtggg
      241 tgacgatcgg gacagggccc gtgtacggca tggcgtccat gccgtgcgtg tcggtcccgc
      301 cgggtccgcc tgccggattg gcccagtgca gggtctggtc cacggcgaac agatgcggca
      361 ggtagtcgcc gtcggcgtcc accagcccgt tgatccactt gacccgcacc ggccggcccg
      421 tcttgccctc gacggtgagc gccgggtagt tgagcgtgcc tccgtcggcg acgggacgcg
      481 ggtcgcgcgt ggacgcgtag ctccacacgg tggtcggatc gatgtcgttc tcctccgccc
      541 actcctcggg caggatgtac tgctggaact gcttgacgac gatctcgtag tagtcgatct
      601 gccgcccgta tggctcagca agcacgccgc tccgcggcat ggccgggggt atcaccagcg
      661 gctcccggta cttggggatg ctcatgggat cgagcgaacc accgggcagc ggcacagcgc
      721 cggccggggc ggacgtgatg gcgaggacga gggtgaggac ggcgagcgaa agcgtcgcca
      781 cgactcccgc cgcccctccc cggctgtcgc cgaaggcggt cttcatgctg ctcctcctgc
      841 tcatcgcccg cgggccctcc gcggacggat gtggtgcggg ggagcagaca gtcgctcacc
      901 cgctggcaag cgtatgacgg tggatacccg ccgaggaccc agaaaacacc gtatccgcag
      961 tgcaaacctc gctgcgggca gggctcggcc gcggtgaggc gcggccggat ggccggtggc
     1021 gccggccgcg cctcgccgct accctcacgc gagaggtatg tgcgccgccg gcgcgaagct
     1081 ctcgcgcagg cccgcgccgt ggcgcgggcc tgttcggcgg tcaagctacg cccccgccgg
     1141 gccgataccc gcaagagagc atcagggcag ccacatggtc gaccacgaat ccatcccccg
     1201 cacggtccac acgacgccgc cgcgccgccg ttcggcggcg ggcgcggcgc tgctcgccgt
     1261 cctgctcctg acgctggcgg ccgtggccgc cgtgacggcc ccgcacgcgg cggccgcccc
     1321 gtcgctgtcc gcgttgcaga aagaggccag gcgcgccaag gccgagatgg cgagcctgca
     1381 gacggacctg caggccgtcg gccggcggct cgccgaggcg cagcagcggc tcgacgaggt
     1441 caaccagcgt ctggtgcgct cgaggatcga cctgaggcgg gccgagaacg agctcgcgct
     1501 ccagcgagag gtcctggccg agcgggcggc ggcgatgtac aagcagggcg acctggactg
     1561 gctcgacctc ctctccacga tccagagctt cgcggacatc gacacgctcc gctcgctgca
     1621 gcgggccctc gccgatcagg accggcgcac cgaggacgat gcccagcggc tcgcccgcga
     1681 ggcgcgccgc ctcgagcgtc gggtggagag cgaccatgag gacgccgtag cggccgaaca
     1741 ggaggtccag aaccagagga tcgagctcga ccagcggctc gcggagcgct cggcgatcct
     1801 ccaggacgtc acccggcgca tcaagaagat cctcgccagc ggcggtctgg gcccggcgct
     1861 ggccatggcc aggaacgggc agttcactca gctcacctgg gcccgggcgc tgctcgtggc
     1921 catgcgcctt ccggtgaccg cggacaacgt cgcggcggtc gcggcgtggg agatggccga
     1981 aggcggtcac tggtacaaca cggcctacta caatcccctc aacaccacgc agagcatgcc
     2041 gggcgccacc gtgttcaact cggtcggggt caaggcctac accagctgga ggcagggact
     2101 cgaggcgacc gtcaagacgc tgcgcaacgg ctactacggc gacatcatcg cggcgctccg
     2161 ccgcggcaac gactccaccg cggtcgcagc cgccgtcggc gcctcgccat ggggcaccgg
     2221 cgacttcagc cgcctgctct gaggtgcatc tcccgcggcg gcgggaagag tacgagcatg
     2281 ttccgccgcc gcagacccag gccaccacag gtggacccta tcgtgaccga ccgccaaggc
     2341 cgcgtcgtcg gcgttcgcac gccgccgccg ccggcgcccg aagagagccg ggccacccgc
     2401 gtgtggcgcc gccgcgagaa cctggcggcc accggcgaac tgcgtcacca ggctgagctg
     2461 cgcaaccagt acgcggcctt ccacgtcgac ccgggcccgt cggtcaggcg atgaacggat
     2521 gagcgccggc tcctggatcc tcctcgccgc cgtcgtcgcc gtcgcagtgc tctggggcgt
     2581 gccgcggttg ctcggccggc gccgggaccg gcgccggcgc gagctcatcc ggcgtctcga
     2641 gcaggcgcgc ggcacgcgcg tcatcacgct gatccaccgc caggagacca tgagccttct
     2701 cggtctcccg ttcggccgct acatcgacat cgacgactcg gagcagctgc tgcgcgccat
     2761 ccggctcacg ccgcccgaga tgccgatcga cctgctcctg cacacgccgg gcgggctggt
     2821 gctcgccagc gaccagatcg cccacgcgct caagcggcac gacggcaggg tgacggtcat
     2881 cgtgccgcac tacgccatgt ccggtggcac gctcgtggcg ctcgccgccg acgagatcct
     2941 catggacccg gacgccgtgc tcggacccgt cgacccgcag ctcggcagcc tgccccgcgg
     3001 gtcgtggccg gcggcatcga tcctcaaggc gctggagcag gacaacccca atcgcgacga
     3061 cgagacgctg atcctcggcg acgtcgcgcg caaggccatc gaccaggtgc gcgcgaccgt
     3121 ccgcgagctg ctggagggcc gcgtcgagga tgccgaagga gagcggctgg cggcgctgct
     3181 gagcgagggt cgctggacgc acgactaccc gatccgcctt gaggaggcgc gggagctcgg
     3241 gctgcccgtc tccgacgccg tgcccgacct cgtctacgag ctgatgacgc tgtacccgcg
     3301 ccgcagccag cggcggccgt ccgtcgagtt catccccacg ccgtaccgca cgcgcggcgg
     3361 cgacggctga ggcgaccgcc acgcatggac gacgatcgcg gccaggccta cctgcgtgag
     3421 acgcggcatc gccgctcgcg tctcgtcggc gggcgcatgg actgggcgca ccagccggac
     3481 tggttcaaga cgtatcccga tgcgccgcgg gtcaagctgc ccgacctggc gctcgacgac
     3541 ggcggcatct tcgacgcgct cgcgcgccgc aggagcgtcc gggcgtactc ggcggagccg
     3601 ctcgcgctcg gcgagctgtc ggcgctgctc tgggcggccg ccggcgtcac ggcgcgccag
     3661 gaaggcttcg ccttccgcac ggcgccgtcg gccggcggcc tctaccccat cgagcactac
     3721 gtggtcgcca gtgacgtcga agggctcgag cagggcctct atcactacga cgtgctcgga
     3781 cgcgcgctcg agcgcctgca cgcgggcgac ctgcgcctgc ccatcgcgcg cgcggcgctg
     3841 gaccagcgcg tcgcggccga cgcgccggcg gtcttcgtct ggacggccgt cctggagcgc
     3901 tcgcgctgga agtacggcga gcgcttcgcc cgctacgtcc tgctcgacgc cggccacatc
     3961 gccgagaacg tcgccctggc ggcgacggcg ctcggcctcg gcacctgcca gatcgccgcc
     4021 ttcttcgacg aggaggcggc ggacctcatc ggcgtcgacc ccgacaggga gccggtcgtc
     4081 tacatgagca cggcaggacg accggccggc gagtagggga gcgccggcct cagccggccg
     4141 gcgccgacgg gacgagccgg ccgcgcggtc agcgcacccg cggggcgatc caggccgcga
     4201 ggtcctcgta gacccgctcg cggccgacct cgttgaagca ctcgtggtag agaccctcgt
     4261 agctgcggat cgtcttgtcc ccagactcgg cacgcgcgaa gagggcctcg atgaaagcga
     4321 cgtccaccac ctggtcgtcc ttgccgtaca gcacgtacag cggcagggcg atgcgttccg
     4381 ccgcggccat ggccgcatcc tgcgcggcca gagtctccgc ggcccagcgg gccggggcga
     4441 cgtggtgcac cagccggtcg gtgacgtagg cgtcgacgac cgtcttgtcg tgcgagagct
     4501 gctccgcctg caacgtgttg ccgatgtcct tgtggggcca gaccaccgcc agtcccttgg
     4561 cggcggccac cctcgccggg ggtaaagcct cggcgagctg gaggaacggc gaggagagga
     4621 tgagcgcgcg cacggcgggc gggcgctcct cggcgaggcg cgcgcagatg agccctccca
     4681 ggctgtgccc gaggaggagg agcggcttcc ccgactggag ccggcgcacc tcgtcgaggt
     4741 acatctcggt gtcgtcgagg tagtcgtcga accgtttgac ctgcccgcgc cggcccgacg
     4801 actggccgtg gccacgcagg tcgtacacgt acaccgccca gccgcgcgcg gccatcgcct
     4861 cgccgaaccg gacatagcgg ccgccgtggt cgccgtaccc gtggaccacg gcgatcacgg
     4921 ccgagggctc gggcagctcc atcggatccg ggccggccgg gagccaggcc tgcaggagca
     4981 ggcgtacgcc gccggccccc aggaactggg tggacgcgtg ccggcaggaa tgcgccgcgc
     5041 cgccggcgcc ggatgtcgcc tccgcgccca tcacgtcagc tcgtgagcgc ggcgcggatg
     5101 gacgcggaga cggcggccag gccgccctcc agctcctccg gcagcggcca cgcgtagccg
     5161 atgcgcatgt ggcgccggtc ctgctcgaac cagtgccccg gccccacgta ggtcccgtgc
     5221 acctcgccga gcactctgta gtactcgtcc acgtcgaccg ggacctccgg cctgatccgc
     5281 gggaagcaca ccacacctcc gcgcggctcg acccactcca ccagctcctc gccggcgatc
     5341 cagtgcctga ccacctgcaa ctgccgggcg atgaacgggt ggagctcggc gagccacgcc
     5401 gcgcgctgct ccagcgcacg cgcggcgatc tcctcgtcca cgacgctgcc gcagatgccg
     5461 atctgctcct tggcggcgag gaacgtcctc atgagctcgc cgtcgcggca gaccagccag
     5521 ccggtgcgga tgcccgggac gccgtacgtc ttgctgagcg agctgacgct gatcacccgc
     5581 tcgctcagcg tcgccgccac cgggagcggc tcgccgaacg tcaactcgcg gtaggtctcg
     5641 tccaccagca gacggcagcc ggcgcgttcg acctcgccga ccaggctccg caggtcggcc
     5701 tctcccatca tcacgccggt cgggttgtgc gggagcgtga tgctcacgta cctcgtgcgc
     5761 ggcgtgacca gcgccatgag cttctcggtg tcgatgcgga agccgtcctc gaaggtcagg
     5821 tcgaggaagc tcacgtctgc ctcgatggcg cgcggcgtct cgatgttggt cgcgtagttg
     5881 ggccggatga cgaccaggtg gtcgcccctg tcgagcagcg aggtggcgac gatgaagagg
     5941 gcctgggcgg cgcctgccgt gacgagcacg tcgtccccgg tcacgccgtc cggcccgccg
     6001 ctctgctccg cgatcaggtc gcggaggcgc tcgttgccgg cgtggtcgcc gtagaagagc
     6061 agcccgtcct cgagggtgac gccgagatcc gagagggagc ggtcccgcac ggagctctcg
     6121 gtgaggttgt agcggatgcg gtcgtagccg aactgctcgg gagactcctc ctcgatgggc
     6181 atgcggcggt acttcacggg cacagacctc ctgacgacgc gcaagactgc cggccaaccc
     6241 tacatccccc ccgggcggcg cgcacggcga cacggcgcgc cggcggagcc ttcgcgcgca
     6301 cgcgcctcgc gcgtcgtcca tgctagagtc aaggccggtc cacgggagga gtcgatggcc
     6361 cgcagccacc gccgtgtcag tgtcaccatc gcgctcgtcg ccgcgctcct gctggcagcc
     6421 gcggcggctg cggcgtgctc ggccagggcg accacggggt cggcctcgct cgccacgggc
     6481 gccgccccga cgctgggcgc cggcgaggac gacgccgacg gcagcggggc cggcgccagg
     6541 ggcgcctcgg gctccgactg gagccgcgtc ctcgccgcac tcgcctggat gcaggccgac
     6601 acgcccacca ggcccgtggt cgtcctgctc ggcggctcgg ccgcccggga gtccaccatc
     6661 agcgaccaga gctggcgcga ccagatcgtc gccgagggcg gtcccgagac gctcgcctgg
     6721 aacatgggct cgcgcaaccg gacgatggcg cagaacgtgg cgctcgtgaa ggccctgccg
     6781 agaggcgccg aggccatcgt ctacatcggc gtcaacctgg gctcgttcac ctcggcgcag
     6841 aagacggcgt ccatcacgct gccctcgccg gcccccgcct cgtccccttc gctgcagcag
     6901 ccgcaccagt acagcacctc gaccggcatc ctcccgacca cgaagaagaa ggccctggtg
     6961 cgggcctggc gcgccgaccg gtacccggtc ttcacgcgca acttcaggac cagcgccgcc
     7021 gtgctccaga cgctcatcag ggtctgcctg gcgcgcggct acaggccggt gctgttcgag
     7081 ctgccgagga acaccgcggt catcggcagc tcgctgagcg cgccgacggc ccggtaccgc
     7141 gacaagtgca gagtgctggc ggcgcagtac aagatcccgt gggtgagctt cgtgaaggcg
     7201 gcggggctgc cgaacgggga cttctacgac ctctggcacc tggtcgaacc gggccgcacg
     7261 gtgtggcagg gcctcctgag cgcgaagacg gcggccctgc tcgagcggta cggctacgat
     7321 ggcggcggct cgtaaggccc tcagccgccg cccggtgcgg gtggcggcca ggctgacgct
     7381 gatcctcctc ctggccgccg ggctggcggc gctcaccctg gtggccgcgc cggcccgcgc
     7441 gggcgacggc gcctccggcg cacccaccac cgcgctcctc ggcgcggccc ccgctccggc
     7501 tcccagcttc gcgcccgcgc tggtcccccc cgtcggcgag gaggcgcgcg gcaccgacta
     7561 cggcaaggac tggcagcact gtgtgcaggt ggtcgccaac ctgcgcgccg cgccccccac
     7621 gactccgctg gtggtgctcc tcggcggctc gtcggcgcgc gagagcacgg tgctcgacga
     7681 ccactgggag cgtcagatca cccgtcgcag cgggtacgtc gtcgacgcct acaacctggg
     7741 ctccaagcac cgctcctacg cccaggacct cgccttcgtg aagctcctgc ccgccggcgt
     7801 gccgaccatc gtctacgtcg gcgtcaacct cggccgtttc tgcctgccgg tgagaccggc
     7861 gagcatcacc ctgcccagcc cccgtacgct gccgccctgg gggcagcacg tgtactccag
     7921 ggaccgcatc cagacccggg ctcagaagcg ctactacgtc tcgtactggc tggcgcgccg
     7981 ctaccccgag ttccgcgcca actacaagtc cgagctcgtg atgctcgaga ggatcatccg
     8041 cgcctgcaag cggcggcacc ttcgggtcgc gctggtcgac ctgccgcgcg acctccccgt
     8101 catcggctcg gctttcgacg cgcccgtgtc ccgctatcac gccggctgcg cgcggctcgc
     8161 ccgcgcctgg gacatcccgt ggctccactt caactcccag gcgcgcttcg ccgaccggga
     8221 tttcttcgac atcttccacc tggtcgagcc cggccgcgtc aagttccaga gcatcctctc
     8281 cgacaggacc atcgggctgc tgaagcggta ccgcatgccc aggcccgagc ccacgccgtc
     8341 tccgtcccct tcgccgagcc cgtcggcgtc gacctccgcg ccggcttcgc cggcgcctgc
     8401 cttctgagcc ggccggcccg tgtccaccct gacactcctc gggatcgccc tcggtctcgc
     8461 catggacgcc ttcgcggtgg ccatcggcgc cggtctccag ctctgcaacg tcaccaaacg
     8521 ccagacgttc cgccttgcct ggcacttcgg cttgttccag gcgttcatgc cgatcctcgg
     8581 ctggctggcc ggcctgaccc tggtcgacta catcgctccg gtcgaccact ggatcgcgtt
     8641 cggcctgctc gcgatcatcg gcggcaagat ggtctacgag gcgctcgagc acgaggagga
     8701 agaggcggac cgctgcgacc cgaccaaggg ctggaggctc gtcatgctct cgatcgccac
     8761 gagcatcgat gctctggcgg tgggcctctc gctggcggtt ctcggcgtga gcatctggtg
     8821 gccggcgctg gtgatcggcg tcgtcgccgg agcgctgacc atggtcggcc tcgagctcgg
     8881 cacccgcttc ggcgccctgc tgggtcgccg catggagatc gtcggcggcc tcatcctcat
     8941 cgggatcggc gtcaagatcc tcgtcgagca cctgaccggg tagtctcgga ggatgtcctt
     9001 cccgcagctc ccggcctatc gcatctccgg cccgctgagc gcgaagctgc gcctggtcac
     9061 gctgctcgcg gtcacggccg tgctctgggt gcacgccaac aacatgagca gccgcttcgt
     9121 cgtcggcgag aacgccgccg aggcgagcgg agcgccgggc gtcgtgggct tcgtcgagta
     9181 cctcgtctcc caggcgctga cgcggtggcc ggcggcagtg ctcttcgcca tctccggctt
     9241 cctcttcttc cacaacctgc agcccctctg gagcgcgtac cggcgcaagt acgcccggcg
     9301 cgtccggacc gtcgtcgtgc cgttcctgct ctggagcggc atcagcctgg ccgtctacct
     9361 ggtcctccag gctgtgccgc agtcggcggc ctacttctcg agcgacttct tcagcggact
     9421 gagcctcggc cacatcgccg acaagctcct cctccacccg gtcgcgtacc cgctctggtt
     9481 cctgcagacg ctgatcgtct gcttcgcgct cgcgccgctg ctgtactggc cggcgcgggc
     9541 gctgcgctgg ctcgcgccgc tgccgttcgc cgcgctctgg atcctgggtg cgccggcggc
     9601 caactggaac gactggaaag gcctcgcctt cttcacgctg ggcgccgtga tcgccctgga
     9661 gcgccgccgc ggcgtgaggc tcacgccggc gccgtgggtc ggccgatggc tgttcccgct
     9721 gtgggtactg gcgtgcgtgg tgttcaccgc ctacctgcgc ggcgagtccg ccttctgggc
     9781 gtacagcctg cacaaggcgc tcatgtgcat ggcggtggcc gccgtgtggt tcgggtacga
     9841 gacgtacctg cggccgctcg agggcagacg cctggtcacg ttcctcctcc ccttcagctt
     9901 cttcatcttc gtggcgcagg agccgctgct gacgacgctc aagcggctcg ggctgcgcga
     9961 gctggggtcg agcgacgcgg cgatgctgtt cgtctacttc gccgcgccgc tgctcacgct
    10021 cgtcgcggtc gtcgcggtcg ctgcggccct gcgcaggtac ctgccgacgc cctacgcgtg
    10081 gctgaccggc gggcgcggag gacggagcgc ccgccgctca cacggtcgcg ggcgagccga
    10141 agaccctgcg ccggcggtcg ccggcctcga gggcgagccc gccctcgccg agaccggcgg
    10201 agcgccgaag ccggtcagac gcggcagctg aagccggcgt gcttctcgaa gtacctctgg
    10261 tggtactcct cggcgcgcca ccacctgccg gcagggacga cctgcgtcac cacgggccgg
    10321 gtgaacacgt ggctctcggt gagctgtgcg atcgcggcga gcgcggcctt ctcctggtcg
    10381 cggtcgtgga agaagaccgc ggaacggtac tggtcgccga cgtccgggcc ctgccggttc
    10441 cactgggtcg ggtcgtgcga gctgaagaag tgggcgagga gctcctgata gctgatcagg
    10501 tccgggtcga actcgaggcg taccgcctcg gcgtgacctg tcccgtgcga gcagacgtcc
    10561 tcgtaggtgg ggttctccgt gctgccgccg gtgtagccga cttcggtgct ggtgacgccc
    10621 cgcagcgcgg cgaagcgctg ttccacaccc cagaagcaac ccgcggcgaa ggtggcggtg
    10681 gccatgatgc gcccgtcctg tggtcggtcg tggcgcgcgc cgccccggcg gcgcgccgtg
    10741 cacccccact gttccccggg cgcgccggag gcacgccccg ggatcccgcg acagctcggc
    10801 ggcgcggccg gcaggcctgc gcgccgggaa gcgtcagacc cgctgcttct cgggttcgac
    10861 ggcccttacc ggcgcggccg ggcgcggctg ccgttgaggc ggctgctgcg gctgccggct
    10921 gagttcgcga agccgccggc gctgagcgcg gttcttcgcc gcggtccacc aggtgcgcga
    10981 gtagtaccag agccagccgt actcgagcat gcggtcccag atcgcctgct tcttggccgc
    11041 ctgcttcttg ctcgcccaca gccacttgtg gaacgcccag acgggccgcc aggtggcgta
    11101 gaagcgcttg ttcgccttga tcgtctcctg gaggaggtgg tacggcgtca tctgcttggg
    11161 gtggaagacg acgtgctgcg cgtcgtagaa cgaccagtcg cgctcgagga tgcggccctc
    11221 cgcgtcgagt tcctcgaact gctgcgtacc ggggagcggc gtgaggatgt tgagcatcac
    11281 cgtgtcgatc ttgttcttct tggcgaactt gacggtgtcg ctcacggagt ccggcgtgtc
    11341 gtgatcggcg cccagcacga acatgccgtg ggcgcggatg ccgtagtcgt ggagcacctt
    11401 gatggcgtgc tcgatgtcgc ccacggtctg cgacttctcg aagctgtcga gtgtggcctg
    11461 gttgaccgac tcgaggccga gagccacgaa gtcacactgc gaggcccgca tgagctccat
    11521 cagttccggg tcacgaacga cgtcggtgcg cacctgggcg ccccacggca cagtgatgcc
    11581 ttcatcgatc atgagctgga gaaggcgctt gagccgtttc ctgttggcgg ccatgttgtc
    11641 gtcgtagaag aagatcttct cgggcttctt ctgcttgatc tcctccacaa cgctctcggc
    11701 actgcggaag cggtacttct tgccgaacat cgccgtgacc gagcagaagg tgcagtcgaa
    11761 ggggcagccc cagctggtca tgatcggcgt gttgctgatg cgctcgtgcc cgtcgaccag
    11821 ggtgaggtcc gggaacggca gcgtgtcgag atcctcgcag cgctcgtgca ggggcgcgtg
    11881 cacgatcttg ccgtcgtcgc cccggtacga gatgccggtg atggagccga gctcgcggtc
    11941 gccggccagc gtttcggcga gctcgaccat gatcatctcg cccgcctcgc cgcgggcgac
    12001 gtagtcggcg tgctccaggg cctcgtcggc catgaaggtc acgtgggagc cgccgatgat
    12061 gacggggatg ccgcggcggc gcagctcgtc ggcgatgcga tacgcctcgg ggatcgtcga
    12121 ggtggtgctg gagaggccga cgaggtcgga cgagtagacg tcggcccagt cgatcggcgc
    12181 cagctgcggg ttgaagatgc gcacgtcgtg ccccgcctcc acgagcgccg cgccgatcat
    12241 cggcaggccg aggcgcacga agtacgactt ggaccagagg tgacggctgg gagagatggg
    12301 ctcgatcagg cggatcttca ttctgggcgt gtccttacgc ggagggtcct tcaggggtcg
    12361 tatgagtctg gagcttctca caaaacgggc gatcgagcca gcatacagga caaaaacggc
    12421 ctccgttgtc acggaccgac attgcacggg aagaaccccg agaagacctt gaagatctcg
    12481 acgaggcatc tggcggcctc cgccctcgcg gctccccgtc cccgatgccc cggctgaggc
    12541 gaacggcgac cagggcagtg tgatctgatg gatgtcgaca gcagaggcag cctcgagccg
    12601 ctcaccccgg gccggcggag cgaccgtatc cgcttctacg gccaggggcg cgtgtgcgcg
    12661 cagcccggct gcaccaccct cctctccatg tacaacccat cgtcgcgatg cgccgtccac
    12721 cagcggcgca gcgacgtgat acccctgcgg cgactcgaat cgcaggaggt cgagcgcggc
    12781 tgctcgcagt gtggcgcccg cttcctgacc accaacccgc ggcgcaagta ctgcagcgcc
    12841 cgctgccgct ctcacgcgtt cgcggagcga gagcgggcgg cgcggctgag cgtcgccctg
    12901 cgcgagcgcg cggcagcctg agccggcaga ccgccccggg cggcagcgtc tgctagccta
    12961 gctgtatgga cgcggccttc ttcgacacgc ccatcgaccg ccggggcacc gccagcttca
    13021 agtgggacat gtacgacggc gacgtgctgc cgctgtgggt cgccgacatg gacttcgcct
    13081 cgccgccgcc ggtggtggag gcgctcgagg ccgtcgcgcg ccgcggcgtc ctggggtacg
    13141 cgctggcgcc ggactcgctg gccgaggccg tcgcgggaca tctcgagcgg cgctacggct
    13201 ggcgcatcga ccccggctgg atcgcctggc tgccgggcat cgtgcccggg ctcaacctcg
    13261 cctgcgacgc gttcgccgcg ccgggggagg cgatcctcac cgtcacccca gtctacccgc
    13321 cgttcctgga ggcgcccggc caccgcggcc ggcgtctgat caccgtgccg gccggccttc
    13381 gggacgggcg ctggcagctg cccctcgacg cgatggaggc ggcggtcacg ccggacaccc
    13441 gcgtcctctt cttctgccac ccccacaacc cgctggggcg cgtgtggcgc ggtgtcgatg
    13501 tggagaccgt gctggacttc tgccgccgcc acgacctcgt cctcgtcagc gacgagatcc
    13561 actgcgacct ggtcctcgac gacctcgtgc acatcccctc ggcgctcgcc ggacctgacg
    13621 accaggaccg catcgtcacg ttcatgtcgc ccagcaagac cttcaacctg cccggcctga
    13681 acctcgcctt cgccgtcgtc gccgacgagg agctgcgccg gcggctgctg cagccgggtg
    13741 agggactgct gccgtttccc ggctgcttcg ccgtcgccgc cgccgaggcg gcctaccggc
    13801 acggcggcga ctggcacgcc gggctgctgg actacctgcg cggcaaccgc gacgcgctcg
    13861 aggcgttcgt cgccgcagag ctcccgcggg tgcgcatgac gcacgtggag gcgacgtatc
    13921 tcgcgtggct cgacgtgcgc gccctcgggc tggaggaccc ggcggccgcg tgccgttccg
    13981 ccggggtggc tctgtcggcc ggggccgcct tcggcgaccc ggcctgcctc cgcctcaact
    14041 tcggctgccg cagggagacg ctggacgagg cgctgcgccg cctcgtcacc gtcctgcggt
    14101 agcggcgcga tcgtgagccc ccagagccgc agcccgagac cggccggccg gcgcccgcgg
    14161 cacgccggca gggcgcgcgt gtaccggcgg cgcaggctct tcgccctcgt cgccgtcgtc
    14221 gcgctcgtcg ccgtgctcgc cggcgtcgtc tgggcggcgg tcggcggctg tggctcggac
    14281 ccggcctccg gcggcagcgc ggcgtcctcc ggcggctcgg cttccgggtc cgcagaaggc
    14341 ggtgaggccg ggctcgcacc ctccccaagc ccgaccgcct ggactggccc gccctcctcg
    14401 tcgctcaagc tcaagctccg caagacgatc gtcgacgcgg acatctcgcc caagtcggtg
    14461 gtgagctccg gcagcggcta cgtgttcgcg cagaacatga tgtacaagca cacgatgacc
    14521 gtgtacgact ccacgacact caggctcgtg gagacgatcc cggattcgat cgacgccgcg
    14581 cggttccgca tcaagggcca cggcgacacg atccaagggg cgcccgtcga gtgcgccttc
    14641 ctccccgacg ggcgcttcgc ctacgtctcc cagtactcca tgtacggcga cggcttcgtg
    14701 gaggggcacg acgtctgctc gccgggcggc gcgtccagga gctacgtgta ccgggtgtcg
    14761 ctcgccgacc tcgaggtcga caaggtcatc aaggtcggcg aggtccccaa gtacgtcgcc
    14821 gtcacgcccg accagaagta cgtcctggtg agcaactggt gctcgtacag cctgagcgtc
    14881 atcgacgtcc acaccagcaa agtcgtgaag gacatctccc tcggtccgta tccccgcggc
    14941 atcgccgtct cggcggactc aaagacggcg tatgtcgccg tcatgggcac ctccgacatc
    15001 gccgcggtcg acctcgagga cttctcggtg gagtggatcc gcggcgccgg cagcgggccg
    15061 cggcacctgt gcatggccgg cgacggcaag tacctgtacg cgacgctcaa caacgagggc
    15121 agcgtcgcca agatcgatcc cgggaccggc acggtcgtcg acaaggtctc caccggctca
    15181 cagccgcgca gcatggcgat cgcgccggac ggcaagtcgc tgttcgtcgt caactacgac
    15241 tcgagcacga tgagccacat ccgcaccagc gacatgaagg tcgtcgacac cctcggcacc
    15301 gactcgctgc ccatcgggat cacctacgac gcccccacca ggagcgtctg ggtctgctgc
    15361 tactccggca gcatcatggt cttcaagcag gtctgaggcg gcggtcagcg cccgacgatg
    15421 gtgcgcaccc cgtcgtcccc gccggccagg accacgtctg ccgcccggcg cgcgaagaca
    15481 ccgctctcga ggacaccggg gatgacgccg agctcggctt cgagccgttc cgggtcgccc
    15541 aggtccaggc ccgtgacgtc gaggatcgcc aggccgtcgt cgctgcggta gccccggcgg
    15601 acggcggagg tcccgccgag cgcgcgcacg cgctcggcca cgaacgccgc cgccatcggc
    15661 agcacggcga gcgggaccgg cacgccggcc gcaccgcgcg cgggatcgcc gagccgatcg
    15721 accaacttgc tctcgtcgac gatgcagacg aagagctcgg cggcgctcgc caccactttc
    15781 tcccgggcga gcgccccccc ggcgcccttg accagccgga gccgggggtc caccgcgtcc
    15841 gccccgtcga cgtacagcgg cagcgacggg gtctcgtcca gcccgaccag ccggatgcct
    15901 gccgcgcgca gcgcggccgc ggatgcctcg gagctcggga cggcggcggc gaccagcttc
    15961 ggccgcgccg ccagttcctc gatgaagaac gccgccgtcg accccgtccc gacgccgacc
    16021 gccctcccct cctgcacgta cgccatcgcc gcgacggccg cggcgcgctt ctgttcgtcc
    16081 cgggtcatac actggtcatg ccccggccgg tctgcggtca ccccgtgacg gccggtggat
    16141 cgatgccgga gccaccccgg cggcggccgc gaggaggagc catgaagccc gtgaacgtgt
    16201 tcaccatcgc caccgagacc gacccggagg acccgccggg ctaccgggcg ggggcggtca
    16261 agctcgcgcc gcgcatcgcc gccgcgcaga tcggcggcag tgtctacgac ctcccgccgg
    16321 gcgagagcgt gtgcccgtac cacttcgagt tcggcgccga ggagtggctg atcgtgttga
    16381 gtggacggcc gacgctgcgc cgcgcctccg gccagagtga gctcgaagag gcgctcgagc
    16441 cgggtgacac cgtgtgcttc ccgcccggcc cttccggcgc ccacaaggtc accaacgcga
    16501 cggaggcccc ggtgcgcatc ctcatgctct ccacgatgcc ggaggtggac gtcagctact
    16561 accccgacag cgagaagttc ggcgtgtggc cgggcggcgg cgtcaagggg ttcctggtcc
    16621 gccgcaggag cgaggtcgac tactacgacg gcgagcgctg accgccgtgg cgcggtcatg
    16681 agcatgcacg agccgacgcc cgaagacgcg gcggtggcgc acgagctggt ggattgggcg
    16741 ctcgcgcggc tcgccgaccg tcgcggcgtc ctcccggcca ggcccgcgtc cggaccgctg
    16801 ccgccgctcg cgccggaagg catcggggcc agagctgcgc tggcgacgct ccgcgatgcc
    16861 gtgatcccca cggcgatccc gccggaccac ccgcggtact tcgccttcgt gccgggcact
    16921 gccacggtag ccgcgacgtt cgccgacatg gcgctgtccg cagcgatgat ctacggcggc
    16981 agccgcctcg aggccggggc cgccgtcgag gcggaggacg ccgtgctccg ctggctggcg
    17041 gacgccgccg gcttccccga atcagccggc ggcaccttcg tcagcggcgg ctccatcgcc
    17101 aacctgagcg ccctcgtggc ggcgcgcggc ggcggagagg acgcccatgg ccgccgcgtc
    17161 atcgtcgccg gcgcgtcggc gcactcgtcc atggcggccg ccgcgcacat catgggttgc
    17221 gagctggtca cggcgcccgc ggccgacgcg cacgggcgcc tggacggccg tgtgctcgag
    17281 atggcgctga ccgggcgcga ccgggctgac atcgtcgccg tggtggcgac ggcgggcgcc
    17341 accaacaccg gcgccttcga cgacctggcc ggcgtcgcga ccatctgcgc cggccacggc
    17401 ctgtggctgc acgtggacgg cgcctacggc ggcgccgcgc tgctctcccc gcgcacgcgc
    17461 ccgctgctcg acggcatcga gggggcggac tcgttcatcg tcgacccgca caagatgctc
    17521 tacacgccgt tcgactgcgc cgccgtcgtc taccgcgact gcacggcggc gcgccgctcg
    17581 ctcacgcaga cggcggacta cctggacgcc atctgcgacg tcgagcgagg agacccatcc
    17641 gacctcgccg tgcacctcac gcggcgggtg cgcggcgtgc cgctctgggc gtcggtcctg
    17701 gcctacggca ccggcgccta cgccgccgcc gtggaccact gcgtggaggt ggcggcgtac
    17761 gccgcgacgc ggatcgccag gacgcccgag ctcgagctcg tgatcgcgcc ggcgttcacg
    17821 gtgctcctgg tgcgccgaac cggctggtcg gcggacgact acgccgcgtg gtgcgcggat
    17881 gcgctggcgc gcggcgtcgc catgctcatg ccgaccaggc acgccggcga gaccgtcctg
    17941 cgcttctgct tcgtcaatcc gctgacgacc cgcgaggacg tggacgtggt gctcagcgac
    18001 ctggcggctt ccagcccatc accgccttga cctcgaggaa gtcctcgagc ccgaaccggc
    18061 cccactcacg gccgttgccg gactgcttgt agccgccgaa gggcgcgcgg gcgtcggtcg
    18121 gcgcgccgtt gaggtgcacc atcccggtgc gcatgcggcc ggccacgcgc cgcgcccgct
    18181 ccagatccgc cgagtgcacg tagccggaga gaccgaagtc cgtggcgttg gccagctcga
    18241 tcgcgtcctc ttcgtcacgg aacggcgtca tgcagagcac ggggccgaag atctcctcgc
    18301 ggaagatgcg catgtcgggc gcgacgtcgg cgaacaccgt gggccgcacg aagaaccctc
    18361 tgtcgaggcc ctcgggacgc gcggggccgc cggcgaccag ccgtgccccc tcggcggcgc
    18421 cgacgtcgat gtagtcacgc accgtgtcga acggcctgcg acccgccacc gggcccatga
    18481 aggtcgcctg atcgagagga tcgccgacgg cgacgccgtc ggcggcgcgc g
//
LOCUS       DASUMX010000038         2545 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_873700, whole genome shotgun sequence.
ACCESSION   DASUMX010000038 DASUMX010000000
VERSION     DASUMX010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_873700"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="VFZ86_01730"
     CDS             complement(<1..333)
                     /locus_tag="VFZ86_01730"
                     /inference="COORDINATES: protein motif:HMM:NF015164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HEX5641039.1"
                     /translation="MFFVPHDWRLALATVLLAGLIDVTLGEPPNALHPVVWMGRLIGA
                     LERRRPRSRPGAEFAYGIMIVLVTAGAAFAAGLALTLVLDRMPWWIALPVGACALKTT
                     FSLRGLVRA"
     gene            complement(361..1242)
                     /locus_tag="VFZ86_01735"
     CDS             complement(361..1242)
                     /locus_tag="VFZ86_01735"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX5641040.1"
                     /translation="MKLYLVRHGQSTGNIGGTLMGQSDHPLTPLGEDQARAAAERLAA
                     FGPMPVYSSDLPRARATAEHIAAAWTADDHGPGAPPEIVLDPRLREISLGDYEGRPWQ
                     EFEADTELTAAFAEDPYRTPLPNGESLEHLEARVHAAVIDVLEPFGVDDTGAACGHAV
                     SQGDASATDRNTEAMRAGAAAAALDGAAAAVAADDEMADDAGLPDTMDASVVATGLVS
                     VEGGGSACLVAHDGPIRAILNHYLGVPPEKWWTLSTTHGGVSLVEFAGGCVTIRFINA
                     TGHLAGLEPDKYVPSDD"
     gene            complement(1271..2140)
                     /gene="cobS"
                     /locus_tag="VFZ86_01740"
     CDS             complement(1271..2140)
                     /gene="cobS"
                     /locus_tag="VFZ86_01740"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HEX5641041.1"
                     /translation="MTDPSDTRVGETTGRPEAPLPAGGGGTGSAGRRATLAITFLTGI
                     PLKVEGEVSPGELWGSMGWYPLVGLVMGAAAWAAYAGLLSFLPALVAATLVVILLELL
                     TRGLHLDGVMDTADGLLSGAPRERALEIMKDHNVGAMGVAAAVLLLVLKVAALGALAR
                     ADAGAPLLAGWCAARTLPALNVYWWPYARPAGTGEAFTREHTPGPLQLAGGFLLAGVV
                     VAGLAGLWAGAAGSWYAGLVVAVVAMAVALGVQAAVAKRLGGLTGDVYGMGIELAEAA
                     ALVTGCAVVGLAT"
     gene            complement(2150..>2545)
                     /locus_tag="VFZ86_01745"
     CDS             complement(2150..>2545)
                     /locus_tag="VFZ86_01745"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019912879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HEX5641042.1"
                     /translation="SDPIDVLAKVGGLDVAGMTGVFLGGAAHRVPVVMDGFISAAAAL
                     AAVRLCHECVDYILPSHVSIEIGHQVVLDELGLVPLFDLQMRLGEGTGAALSMSIIEA
                     SARILSEMATFESAGVAGDPSEADAELTR"
BASE COUNT          351 a          946 c          931 g          317 t
ORIGIN      
        1 ggcgcgcacc agcccgcgca gcgagaaggt cgtcttgagc gcgcaggcgc cgacgggcag
       61 cgcgatccac cagggcatcc ggtccaggac gagcgtgagg gcgaggccgg cggcgaacgc
      121 cgctccggcc gtcaccagga cgatcatgat gccgtaggcg aactcggcgc cgggccggct
      181 acgcggccgg cgccgctcca gcgcgccgat cagccggccc atccacacta cagggtgcag
      241 cgcgttgggc ggctccccca gggtcacatc gatgagcccc gcgagcagca ccgtcgccag
      301 cgcgaggcgc cagtcatgag ggacgaagaa cactgcgggc agaagcgggc gcggagcgcg
      361 tcagtcgtcg gaggggacgt acttgtccgg ctcgagtccc gcgaggtgac cggtcgcatt
      421 gatgaagcgg atggtgacgc agccgccggc gaactcgacc agtgacacgc cgccgtgggt
      481 ggtggacagc gtccaccact tctcgggcgg cacgcccagg tagtggttga ggatggccct
      541 gatcggcccg tcgtgggcga cgaggcaggc gctgccgccg ccctcgacgg acaccaggcc
      601 ggtcgcgacc acgctcgcgt ccatcgtgtc cggcagccct gcgtcgtccg ccatctcgtc
      661 gtccgcagcg accgcggcgg cggcgccgtc gagggcggcc gcggctgcgc ccgcccgcat
      721 cgcctcggtg ttgcggtccg tggcgctcgc gtcgccctgg ctcaccgcgt ggccgcaggc
      781 cgcgccggtg tcgtcgaccc cgaacggctc gaggacgtcg atcaccgcgg cgtggacgcg
      841 cgcctcgagg tgctcgagcg actcgccgtt gggcaggggt gtgcggtacg gatcttcagc
      901 gaacgccgcc gtgagctccg tgtcggcctc gaactcctgc cacgggcgac cctcgtagtc
      961 gccgagcgag atctcgcgca ggcgcgggtc gaggacgatc tccggcggcg cgccggggcc
     1021 gtggtcgtcg gccgtccagg cggcggcgat gtgttcggcc gtcgcccggg cacgcggcag
     1081 gtcgctcgag tagaccggca tgggtccgaa cgctgcgagt cgctccgcgg ccgctcgcgc
     1141 ctggtcctcc ccgagcggcg tcagggggtg gtcgctctga cccatcagcg tgccgccgat
     1201 gttgcccgtg gactgtccgt gccgcaccag atacagcttc atgtgtgatc ctcggtcgct
     1261 cttgccctga tcatgtcgcg agaccgacga cggcgcaccc cgtgaccagg gcggccgcct
     1321 cggccagctc gatgcccatg ccgtagacgt cgccggtgag gccgccgagc cgcttcgcca
     1381 cggcggcctg tacgccgagc gcgacagcca tcgccacaac cgcgacgacg agcccggcgt
     1441 accaggagcc ggccgcgccg gcccagaggc cggccagccc agcgacgacc acgccggcca
     1501 gcaggaagcc gccggccagc tgcagcggcc ccggcgtgtg ctcccgcgtg aacgcctcgc
     1561 cggtgccggc ggggcgcgcg tacggccacc agtacacgtt gagcgccggc agcgtgcgtg
     1621 ccgcgcacca gccggcgagc agcggcgcgc ccgcgtcggc gcgcgcgagc gcgcccagag
     1681 ccgcgacctt gagcaccagc agcagtaccg ccgccgccac gcccatcgcg ccgacgttgt
     1741 ggtccttcat gatctcgagc gcgcgttcgc gcggcgcgcc gctcaggagg ccgtcggcgg
     1801 tgtccatcac gccgtcgagg tgcagaccgc gcgtgagcag ctccagcagg atgaccacga
     1861 gcgtcgccgc gaccagagcg ggcaggaacg agagcagccc cgcgtatgcg gcccaggccg
     1921 cggcgcccat gacgagcccg accagcgggt accaccccat gctcccccag agctcgcccg
     1981 ggctgacctc gccctcgacc ttgagcggga tgccggtgag gaaggtgatg gccagcgtcg
     2041 cgcgacggcc ggcggagccg gtgcctccgc cgccggccgg cagcggcgcc tccggccgtc
     2101 cggtcgtctc gccgacgcgc gtgtcgctgg gatccgtcac gctccgacct taccgcgtca
     2161 gctcggcgtc tgcctcggag gggtcgccgg ccacgccggc gctctcgaag gtggccatct
     2221 cgctgaggat gcgggcggag gcctcgatga tgctcatcga cagcgctgcc cccgtcccct
     2281 cgcccagccg catctgcagg tcgaacagcg gcaccaggcc gagctcgtcc agcaccacct
     2341 ggtgaccgat ctcgatgctc acgtggctgg gcaggatgta gtcgacgcac tcgtggcaga
     2401 ggcggacggc cgcgagcgcc gcggcggcgc tgatgaaccc gtccatgacg acggggaccc
     2461 ggtgcgcggc gccgccgagg aacacgccgg tcatgccggc gacgtcgagc ccgccgacct
     2521 tggcgagcac gtcgatggga tcgga
//
LOCUS       DASUMX010000039         7605 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_729956, whole genome shotgun sequence.
ACCESSION   DASUMX010000039 DASUMX010000000
VERSION     DASUMX010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 7605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7605
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_729956"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VFZ86_01750"
     CDS             complement(<1..228)
                     /locus_tag="VFZ86_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HEX5641043.1"
                     /translation="MTTPATPQDAATAALADVAERLQRRLLPAAPPEMAGLDIAFTYR
                     SASAGVLSGGDFVDYYTRPPSGSLAFAIGDVA"
     gene            complement(225..767)
                     /locus_tag="VFZ86_01755"
     CDS             complement(225..767)
                     /locus_tag="VFZ86_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641044.1"
                     /translation="MSVMERPDVENIPGDLDRFLGNFFSRRGWSSEIKYDPAEDRLYL
                     EVRLADARLSADDRFFSLVEYFARAQDSVLRRHSGLPLQARVFADDGRDLTGTLHARG
                     SSYLDDNARGNGMRRRLLLLSFRRRLLARVLPGVLLWALVFTVLVGVVGLRLEVVLAV
                     AVGALVIQAIVMWSAAARRR"
     gene            934..2199
                     /locus_tag="VFZ86_01760"
     CDS             934..2199
                     /locus_tag="VFZ86_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011552757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEX5641045.1"
                     /translation="MKIAVIGLGYVGLPLAMVFAEAGIEVVGVEAVASRCDEINAGRS
                     YIQDVSSDALKVVVDKGLIRATTDYAATEDCDADIICLPTPLNANREPDLTLVKDATE
                     KLAGHLRRGQLVTLESTTYPGTTRDELAPILEAGSGLTAGVDFHLAFSPERVDPGRTD
                     YTTRSTPKVVGGLTPACTARALEVYGPALDHVVPVSTPEVAEMTKLLENIFRSVNIAL
                     MNELAMLCDRMDIDVWEVIDAAATKPFGFMKFQPGPGLGGHCIPIDPFYLSWKAREFD
                     FWTEFIELAGKVNENMPYFCVEKLHRALNTRGKSLHGAKVLVLGVAYKADIDDLRESP
                     ALKVIKLLRARGARVDYHDSYCPELPEFGLSSVPIDDHRSLAAYDVVTIVTAHSDIDY
                     RGVVHDADLVIDFRNATAGIPSAGNVWKL"
     gene            2216..3241
                     /locus_tag="VFZ86_01765"
     CDS             2216..3241
                     /locus_tag="VFZ86_01765"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX5641046.1"
                     /translation="MSDKVRVGVVGLGYWGPNLARNFDRLPGAELAYCCDLDEANLAK
                     AHSLYPAATVTDDYAQLLGDDALDAIVVATSVPTHYALGKKALEAGKHTFIEKPIALK
                     AADAQDLLATADDYGVKLMVGHLLEYHPAVAKMKELIDSGELGKVFYAYANRLNLGKV
                     RTDENALWSFGPHDISVLNYLTGDEPVEVSARGECYLQDAVEDVVFGYIKYKSGIVGH
                     LHVSWLDPHKSRKITVVGEKKMVVFDDMESERKITVYDKGATTTRTKFETYGEFVTLH
                     FGDIHIPKIGNDEPLRLETQHFIDCIVNDETPRSDGRDALNVVKVLDAMEISLREGGR
                     PVKTTEL"
     gene            3250..3960
                     /locus_tag="VFZ86_01770"
     CDS             3250..3960
                     /locus_tag="VFZ86_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HEX5641047.1"
                     /translation="MYPSPLGHNLLLGEGVTIIGADVTFGANVVVHADTVIGAGCYIQ
                     DGAVLGKVPSLSPTSTAKRGELPPLVLGPGCVVSTGAVVYRGTQLGAGCIIGDYAGVR
                     ERCTLGEKVVVGRGSVVENDTTIGDGTKIQTNVYITAYMTIEDRCFIAPCVQTTNDNY
                     MGRTEKRHELIKGATIRRGARVGGGVVLCPGIEIGEEAFIAAGAVVTKDAPARKVLMG
                     VPARVVRDVYEDELLENQ"
     gene            complement(3986..5305)
                     /locus_tag="VFZ86_01775"
     CDS             complement(3986..5305)
                     /locus_tag="VFZ86_01775"
                     /inference="COORDINATES: protein motif:HMM:NF020036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX5641048.1"
                     /translation="MPSFRIPPPLRHVLPFREQDVSLSSPGFAGSQPQPPITAKDKRL
                     QAFLSVSFGVLYDWNIETGAIAFTDEVDAMLGLPPGGFPRGIEGWLESIHPDDHEATM
                     EALSAAILDGVPFSCEYRLRHADGSWATVSDQGVLLTNRSGRATNMIGAMRDVTRERA
                     SQAARREADELRSVLFRLPSPAMQVDERGVYVDADSHALAFFERSRDEMLAATVRDDF
                     PEAVAATIVSAEPGAAVRELEVACRVGGSTKHMLLSIVPTRIGGRRGCFLLGADITGQ
                     KGMQEALARSERALRHQATILDERNAALKVLLEQREQDHKELEERIVSNLDQIIEPTL
                     ERLSRALRHRPERLDVDALRGNLREIVGPFGQRLAQRSTSEEPLTRREKEVAALVRQG
                     RTSAEIAEALHVSRAAVAFHRGNIRRKLGVPKGGPQLATHLDALSHD"
     gene            5543..5890
                     /locus_tag="VFZ86_01780"
     CDS             5543..5890
                     /locus_tag="VFZ86_01780"
                     /inference="COORDINATES: protein motif:HMM:NF018008.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5641049.1"
                     /translation="MEFRHVAASDIATTPLDVGEESKLSLVDVFDRTQGAPFCAGICE
                     VFPGAPVDFDYDDDAAACYMIEGEITLTENGATRSFLPGDVVYIPQKQGLVVFWSTTT
                     YGRFFYVTYPHWR"
     gene            5894..7573
                     /locus_tag="VFZ86_01785"
     CDS             5894..7573
                     /locus_tag="VFZ86_01785"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX5641050.1"
                     /translation="MSPEPEHHHGLFHDEMDAQHKKDMEQLAKFGYKQDLRRSLGFFS
                     TFAIAFSFISATNGFYALFYYGLDIGGPAGLIWQWPIIVFGQFMVALVFAEAASHYPL
                     AGGIYQWAKRFMGGDYGWWVAWVFATALVVTVAAVAFGVAPIVCALMGWDASNTTTLL
                     IIAVCYAVAPMILNVLSVKVTAFFNNLGTVTEIIGLVVISIALYAAVLFGKGDHQSLS
                     VLFDTTPGAGSGALFGYGSIFLASMLTGAWVLYGFDTAGGLAEETINPTREVPRAMIF
                     AIAITAIISAFWLVAMVLAIPDVAETQAQGTGAIAFIFDAHFPTWVTDTFLVTVCIAI
                     FVCCLAIQAATSRLLFAFGRDKMIPASKFFGYVHPSTKTPLASAIFVAAAAIVVLLYV
                     NLGGSDPFIAIARVTAWATAGTYIAYQMVVFGGLIARGRGWPKDRAYFNLGKWGWPVN
                     ILALVYGVFMIINLVWPRTPDAVWYDNYLVIVSLVVVVIAGAIIYAIQKARGVDLSAT
                     IKEIDESPAEGAAMEAMAAGEAGKILPNADPPAGAVTPMTPDDQGKGGLTL"
BASE COUNT         1227 a         2655 c         2550 g         1173 t
ORIGIN      
        1 ggcgacgtcg ccgatggcga acgcgaggct gccggacggc ggccgcgtgt agtagtcgac
       61 gaagtcgccg ccgctgagca cgccggcgct ggcggagcgg taggtgaagg cgatgtcgag
      121 gccggccatc tcgggcggcg ctgccggcag gagacgccgc tgcaggcgct cggccacgtc
      181 ggccagcgcc gcagtggcgg cgtcttgtgg ggtggcgggg gtcgtcaccg tcgtcgcgcc
      241 gccgcggacc acatcacgat cgcctggatg acgagcgcgc cgacggcgac cgcgaggacg
      301 acctcgagac gcaggcccac gacaccgacg agcacggtga agaccagggc ccagagaagc
      361 actcccggca ggactcgtgc gaggagccgc cgccggaagc tcagcagcag cagccggcgg
      421 cgcatgccgt tgccgcgcgc gttgtcgtcg aggtagctgg agccgcgcgc gtgcagcgtt
      481 cccgtgaggt cgcgcccgtc gtcggcgaac acgcgtgcct gcagggggag gccggagtgc
      541 cggcgcagca ccgagtcctg cgcgcgcgcg aagtactcga cgagtgagaa gaaacggtcg
      601 tcggcggaga gcctcgcgtc cgccagccgg acctcgaggt acaggcgatc ctcggcgggg
      661 tcgtacttga tctcgctgct ccagccgcgg cggctgaaga agttgcccag gaaccggtcg
      721 aggtcgcccg ggatgttctc gacgtccggt cgctccatca cgctcatgct ccgattctac
      781 ccatcccctc ctccatcggc agccggacgc gatcggtgga gtcgaaggag gaggccgggc
      841 gccgtggttc gaagcggcgc aggtgaatac gatagaatgc ccgacttggt ggggcggccg
      901 tccgccgcct cgggcgaatc tctaggagca cacgtgaaga tcgcagtcat cggcctcggc
      961 tacgtcggcc tgccgctcgc gatggtgttc gccgaggccg gcatcgaggt cgtcggtgtc
     1021 gaggccgtgg cctcgcgctg cgacgagatc aacgccggcc gcagctacat ccaggacgtc
     1081 tccagcgacg cgctcaaggt cgtggtcgac aagggcctca tccgcgccac gaccgactac
     1141 gccgccaccg aggactgcga cgccgacatc atctgcctgc cgaccccgct caacgccaac
     1201 cgcgagccgg acctcacgct ggtgaaggac gcgaccgaga agctggccgg gcacctgcgg
     1261 cgcggccagc tcgtcaccct cgagagcacc acgtatccgg gtacgacccg cgacgagctg
     1321 gcgccgatcc tcgaggccgg ctccggactc acggccgggg tcgacttcca cctcgccttc
     1381 tcgcccgagc gggtcgaccc cgggcgcacc gactacacca ccaggagcac gcccaaggtc
     1441 gtgggcggtc tcacgcccgc ctgcacggcg cgcgcgctcg aggtctacgg ccccgcgctc
     1501 gaccacgtcg tgccggtgag cacgcccgag gtcgcggaga tgaccaagct gctcgagaac
     1561 atcttccgca gcgtcaacat cgcgctgatg aacgagctcg cgatgctctg cgaccgcatg
     1621 gacatagacg tgtgggaggt catcgacgcc gccgcgacca agcccttcgg gttcatgaag
     1681 ttccagcccg gcccgggcct cggcggccac tgcatcccga tcgacccgtt ctacctgagc
     1741 tggaaggcgc gcgagttcga cttctggacg gagttcatcg agctggccgg caaggtcaac
     1801 gagaacatgc cgtacttctg cgtcgagaag ctccaccgcg cgctcaacac gcgcggcaag
     1861 agcctgcacg gggccaaggt gctcgtcctc ggagtggcct acaaggccga catcgacgac
     1921 ctccgcgaga gccccgcgct caaggtgatc aagctgctcc gcgcgcgcgg cgcccgagtg
     1981 gactaccacg actcgtactg ccccgagctg cccgagttcg gcctcagctc ggtgcccatc
     2041 gacgaccacc gctcgctcgc cgcctacgac gtggtgacga tcgtgaccgc gcactccgac
     2101 atcgactatc gcggggtcgt gcacgacgcc gatctcgtga tcgacttccg caacgccacg
     2161 gccggcattc ccagcgccgg caacgtctgg aaactgtaag aggggagacc cactcgtgag
     2221 tgacaaggtc cgcgtcggcg tggtcgggct cggctattgg ggcccgaatc tcgcccgcaa
     2281 cttcgaccgc ctgcccggcg ccgagctcgc gtactgctgc gatctcgacg aggccaatct
     2341 ggccaaggcg cacagcctgt acccggcggc gacggtcacc gacgactacg cccagctgct
     2401 cggcgacgac gcgctcgacg cgatcgtcgt cgccacctcg gtgcccacgc actacgcgct
     2461 cggcaagaag gcgctcgagg ccggcaagca caccttcatc gagaagccca tcgcgctcaa
     2521 ggccgccgac gcgcaggacc tgctggccac cgccgacgac tacggtgtca agctcatggt
     2581 cggccacctg ctcgagtacc accccgcggt cgccaagatg aaggaactca tcgactcggg
     2641 cgagctcggc aaggtcttct acgcctacgc caaccggctc aacctcggca aggtgcgcac
     2701 cgacgagaac gcgctctgga gcttcggccc gcacgacatc tcggtgctca actacctcac
     2761 cggcgacgag ccggtggagg tcagcgcccg cggcgagtgt tacctgcagg acgcggtcga
     2821 ggacgtcgtc ttcggctaca tcaagtacaa gagcggcatc gtcggccacc tgcacgtcag
     2881 ctggctcgac ccgcacaaga gccgcaagat caccgtcgtc ggcgagaaga agatggtcgt
     2941 cttcgacgac atggagtccg agcgcaagat cacggtgtac gacaagggcg cgaccacgac
     3001 gcgcaccaag ttcgagacgt acggcgagtt cgtcaccctg cacttcgggg acatccacat
     3061 ccccaagatc ggcaacgacg agccgctgcg cctcgagacc cagcacttca tcgactgcat
     3121 cgtgaacgac gagacgccgc gcagcgacgg gcgcgacgcg ctcaacgtgg tcaaggtcct
     3181 cgacgccatg gagatcagcc tgcgcgaggg cggccgtccc gtgaagacca cggagctgtg
     3241 acggtgacga tgtatccgag cccgctcggg cacaatctgc tcctcggcga gggcgtcacc
     3301 atcatcggcg ccgacgtgac cttcggagcc aacgtggtcg tccacgccga cacggtcatc
     3361 ggcgccggct gctacatcca ggacggcgcc gtgctcggca aggtgccgag cctctcgccg
     3421 accagcaccg ccaagcgcgg cgagctgccg ccgctcgtgc tgggtccggg ctgcgtcgtc
     3481 agcaccggcg ccgtcgtcta tcgcggcacc cagctcggcg caggctgcat catcggcgac
     3541 tacgcggggg tccgcgagcg ctgcacgctg ggcgagaagg tcgtcgtcgg gcgcggcagc
     3601 gtcgtcgaga acgacacgac gatcggcgac ggcaccaaga tccagaccaa cgtgtacatc
     3661 accgcgtaca tgaccatcga ggaccgctgc ttcatcgcgc cctgcgtgca gaccaccaac
     3721 gacaactaca tgggccgcac ggagaagcgc catgagctga tcaaaggcgc gaccatccgc
     3781 cgcggcgccc gcgtcggcgg cggcgtggtg ctctgccccg gcatcgagat cggcgaagag
     3841 gcgttcatcg ccgccggcgc ggtcgtcacc aaggacgcgc cggcccgcaa ggtcctgatg
     3901 ggcgtgccgg cgcgcgtcgt gcgcgacgtc tacgaggacg agctgctcga gaaccagtag
     3961 ggcggcgctg ccgggctccg gcgcctcaat cgtgggagag cgcgtccagg tgcgtcgcga
     4021 gttgtggccc gcccttgggc acgccgagct tacgccggat gttgccgcgg tggaaggcga
     4081 ccgccgctcg gctcacatgc agcgcctcgg cgatctctgc gctcgtgcga ccctgccgca
     4141 ccagggccgc gacctccttc tcgcgtctgg tgagcggctc ctccgacgtc gaccgctgcg
     4201 cgagccgctg gccgaacggg ccgacgatct cgcgcaggtt gccgcgcagc gcgtcgacgt
     4261 cgagccgttc gggacggtgc cgcagggcgc ggctcagccg ctcgagcgtc ggctcgatga
     4321 tctggtcgag gttggagacg atgcgctcct cgagctcttt gtggtcctgc tcccgctgct
     4381 cgagcaggac cttgagggcg gcgttgcgct cgtcgaggat cgtcgcctgg tgacgcagcg
     4441 cgcgctccga gcgcgccagc gcctcctgca tccccttctg accagtgatg tcggcgccga
     4501 ggaggaagca gccgcggcgc ccgccgatcc gcgtgggcac gatgctgagc agcatgtgct
     4561 tggtgctccc tcccacccgg caggcgacct cgagttcgcg cacggcggcg cccggctccg
     4621 ccgagacgat cgtcgccgcg accgcctcgg ggaagtcgtc tcgaacggtc gccgcgagca
     4681 tctcgtcccg tgaccgctcg aagaaggcca gcgcgtgcga gtcggcatcg acgtagacgc
     4741 cacgctcgtc cacctgcatc gccgggctgg ggagcctgaa cagcacgctc cgcagctcgt
     4801 ccgcctcgcg ccgcgcggcc tgcgacgcgc gctcgcgggt cacgtcgcgc atcgccccga
     4861 tcatgttggt ggcgcgacca gaccggttgg tgaggagcac gccctggtcg ctgacggtgg
     4921 cccacgaccc atcggcgtgt cgcagccggt attcgcagct gaacgggacc ccgtcgagga
     4981 tggccgcgga gagcgcctcc atcgtcgcct cgtgatcgtc cggatggatg ctctcgagcc
     5041 acccctcgat gcctcgcggg aacccgccgg gggggagccc cagcatggcg tcgacctcat
     5101 cggtgaaggc gatggcgccg gtctcgatgt tccagtcgta gaggacgccg aagctgacgc
     5161 tcaggaaggc ctgcagccgc ttgtccttgg cggtgatggg gggttgtggc tggctgccgg
     5221 cgaacccggg tgagctcagg gacacgtcct gctcgcggaa gggcagcacg tggcgcagcg
     5281 gcggcggtat gcgaaagcta ggcattacct cacactctcc tcgtacttta ctggtcttgc
     5341 tcccgcgcat tgtccggcta gagtcgtcgt gagtcaacgc ccatcacagg gggtcggccg
     5401 ctgccgagcg cggcgcggtg gatcgggtcg agacgaaagg ggggcacggg cagatcactc
     5461 cgccggcgtc tgccggcact acctcaccgt cgggtgcgca ggtgcaccgc agcgtgacgt
     5521 ctacgccagc aaggaggtag agatggagtt ccgccatgtg gcggcgtccg acatcgccac
     5581 gacgccactg gacgtcggcg aggagagcaa gctgagcctg gtcgacgtct tcgaccggac
     5641 gcagggcgca ccgttctgcg ccgggatctg cgaggtcttc cccggcgccc ccgtcgactt
     5701 cgactacgac gacgacgctg cggcctgcta catgatcgag ggggagatca cgctcaccga
     5761 gaacggcgcg acgcgctcgt tcctgcccgg cgacgtcgtc tacatccctc agaagcaggg
     5821 tttggtggtc ttctggagca ccaccacgta cggccgcttc ttctacgtca cctacccgca
     5881 ctggaggtga gccgtgtcac cggaacctga gcatcaccac ggcctgttcc acgacgagat
     5941 ggacgcgcag cacaagaagg acatggagca gctggccaag ttcggctaca agcaggacct
     6001 gcggcgctcg ctgggcttct tctccacctt cgccatcgcc ttcagcttca tctcggcgac
     6061 caacggcttc tacgcgctct tctactacgg cctggacatc ggcgggccgg ccggcctcat
     6121 ctggcagtgg ccgatcatcg tgttcggcca gttcatggtc gcgctggtgt tcgccgaggc
     6181 ggcctcgcac tatccgctgg ccggcggcat ctaccagtgg gccaaacgct tcatgggcgg
     6241 cgactacggc tggtgggtcg cctgggtctt cgccaccgcg ctcgtggtca cggtcgcggc
     6301 cgtcgccttc ggcgtggcgc cgatcgtctg tgctctcatg ggctgggacg cgagcaacac
     6361 gacgacgctg ctcatcatcg cggtctgcta cgccgtggcg ccgatgatcc tgaacgtcct
     6421 cagcgtcaag gtcacggcct tcttcaacaa cctcgggacc gttaccgaga tcatcgggct
     6481 ggtggtgatc tccatcgcgc tgtacgccgc ggtcctcttc ggaaagggtg atcaccagag
     6541 cctgagcgtg ctgttcgaca cgacgccggg cgccggatcg ggcgcgctct tcggctacgg
     6601 cagcatcttc ctcgcctcga tgctcaccgg cgcctgggtg ctgtacggct tcgacaccgc
     6661 cgggggactc gcggaagaga cgatcaatcc gacccgtgaa gtgccgcgcg ccatgatctt
     6721 cgcgatcgcc atcaccgcga tcatcagcgc gttctggctg gtggcgatgg tcctcgccat
     6781 cccggacgtc gccgagacgc aggcgcaggg gacgggggcg atcgcgttca tcttcgacgc
     6841 gcacttcccg acctgggtca cggatacctt cctggtcacc gtgtgcatcg ccatcttcgt
     6901 ctgctgcctg gcgatccagg cggcgacgtc gcggctgctg ttcgccttcg gccgcgacaa
     6961 gatgatcccc gcctcgaagt tcttcggcta cgtgcatccg agtaccaaga ccccgctggc
     7021 gtcggcgatc ttcgtcgccg cggcggcgat cgtcgtgctg ctgtacgtga acctcggcgg
     7081 cagcgacccg ttcatcgcca tcgcccgtgt caccgcctgg gccaccgccg gtacctacat
     7141 cgcctaccag atggtggtgt tcggtggcct catcgcgcgg ggccggggct ggccgaagga
     7201 cagggcctac ttcaacctgg gcaagtgggg ctggccggtc aacatcctcg ctctcgtgta
     7261 cggcgtcttc atgatcatca acctggtgtg gccgcggacg ccggacgccg tgtggtacga
     7321 caactacctc gtcatcgtct ccctggtggt cgtcgtcatc gccggcgcca tcatctatgc
     7381 gatccaaaag gcccgcggcg tcgatctcag cgcgaccatc aaggagatcg acgagtcgcc
     7441 ggccgagggt gcggcgatgg aggccatggc ggccggcgaa gcgggcaaga tccttcccaa
     7501 cgcggaccct cccgcgggcg ccgtgacccc gatgactccg gacgaccagg gaaagggggg
     7561 cctcacgctc tgacggcctg acagcgcgac cagccgcccg tgagg
//
LOCUS       DASUMX010000040        10616 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_2023934, whole genome shotgun sequence.
ACCESSION   DASUMX010000040 DASUMX010000000
VERSION     DASUMX010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 10616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10616
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2023934"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2851)
                     /locus_tag="VFZ86_01790"
     CDS             complement(<1..2851)
                     /locus_tag="VFZ86_01790"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005261657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HEX5641051.1"
                     /translation="MAGYQAIDAARHDFDGLVQLIKDKQVKSEGAILVEHDESGDVRV
                     SETGDHLGRKGMGWGGGVGVLVGLAAPPLLAATAVGAAAGGIVGKFAKHKVESGLESG
                     LGEKLKPGTAAVIVIVAEADRLTAEKALAGSPAKSVAPMEKHGLKGLKEALAEAAGKF
                     TPDRTVLPIPDKKFGGACGRTIAESAADWSFIPGVSAPESAPNVLLVLIDDAGFGGPD
                     TFGGGIATPNLTRVQDMGLTYNQFHVTAVCSPTRAALLTGRNHHRVGMGAIAEFPGPF
                     PGYTGTRPRSCTALPRILKENGYVTGGFGKWHMTPGRELGAAGTFDHWPQGWGFDHWW
                     GFLTGAAGQYDPIITQDNSTLGVPEGEDGKLYYFPDDITDKSVEWLHAVRAHDPQKPW
                     FVYYSTGCSHAPHHVAKEWADKYKGKFDTGWDVYREQTLERQKKLGIVPPDTELSERP
                     DLFPAWDSLTATQKKLYARQMEVFAGFSENADWNVGRLLDAVEEMGELDNTLVLYIWG
                     DNGASMEGTLTGSFNETTFFNGVVLEADEQLEIIEKYGGVEELGGFHTAPHYAAAWAH
                     ANNTPFQWGKQMASHRGGTVDPMVVAWPGRITPDKAVRSQFTHCIDVVPTILELAGIP
                     EPTTVDGIDQEPMDGTSFAYTLDDALAPEQHTVQYFEMYGSRAIYKDGWWACAKLDKL
                     PWDFKPETLRRFAPGGTWDPEKDTWELYYLPDDFSQAHDVAAENPEKLAELKELFWQE
                     AERNRALPLLGGYAVFFGMLPPMPTVTRMEFAGDVQNIQTTMIPRIIGRSYAIEGELH
                     VPEGGAEGVVCAFADFIGGFSLWVDENGLLNHTYQFLGVETYKQTSTEPIPTGDVKVK
                     MLFEADEPKPGAGGKVTLWADDKQIGEGRLDKTISLIFTTYAGMDMGRDNGGVVDLAY
                     EDRAPYAFTGTVKKVTFDLQPAHHEAEKALHEHAVT"
     gene            3067..3702
                     /locus_tag="VFZ86_01795"
     CDS             3067..3702
                     /locus_tag="VFZ86_01795"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5641052.1"
                     /translation="MDKGKRHRPRGGRERTTEAILDAAEKLFSQRGFAAVSVRDIAAE
                     AGVSHALVHRYLGSKAQVYRTMLLRRETVIRDAAPDVDDLIEATKLMLREAVLHQRGY
                     VRMLASSALDGLSFERTVGRFAATERLVEIAEKVVAGEGEARGPDARDPRFVIASIVA
                     MLLGWSAAREWILKAVDLEPMSDEEFCDEFESIVLDLERLYFPTLGGPAAE"
     gene            3836..4336
                     /locus_tag="VFZ86_01800"
     CDS             3836..4336
                     /locus_tag="VFZ86_01800"
                     /inference="COORDINATES: protein motif:HMM:NF018590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HEX5641053.1"
                     /translation="MSELMVLGFENEAAAEEFGVTLAQMQKDMIVQLQDASMVVRDPD
                     GKPHVKHGNKLVGAGALGGAFWGMLFGLLFFVPFLGLAIGAGMGALFGKLGKTGIDRQ
                     VLEQMGDAVPPGKAGWFLLIGQMTEDKFLAAVEGTQATLVRSNLSHDQEEELKQAFGT
                     EKHAKD"
     gene            4441..6471
                     /locus_tag="VFZ86_01805"
     CDS             4441..6471
                     /locus_tag="VFZ86_01805"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEX5641054.1"
                     /translation="MAETEAKAYGAIPQWSPARPRIRPHRVLLQWVTSALALYIAAMI
                     VPGVKIETLLGAFIAATLIAVLNAVLPPLIAALRLPYMLAVGFLLVLLLDAWMVKVAA
                     DLSDHTFTVDRFFWALVVALVAAAVTVALEVLLGTNDEDVYSLRVTQRIARRSRDRVV
                     TSVPGIIYLEIDGLARPVLQRAVRDGHAPEMGRWLASGTHRLSEWETDLSSQTGASQA
                     GLLLGSNDDIPAFRWVEKESGRIMTCSAPADCALIEERHSTGRGLLADGGASRSNLLS
                     GDADHVILTVSRMEAEKKANPGYRAYLADGYNVTRTFVLFFYEVVLELAGAARQRRRD
                     VQPRGHRSLSYAFMRAGLSVFVRDFTVHSIISDMMMGVPAVYACFAGYDEVAHHSGLE
                     RADTMDVLRKLDQQFGRVARARRYAARPYEIVVLSDHGQTQGATFKQRNGYGLDQLVE
                     RNLREAGVENLGSGDENDSAVSKAVREATGREQKDEDKHQVGDRKVIVMGSGNLGLIY
                     LMDEPRRLTMEEIDERHPDLLPALRAHPHVGWLLVRSAEHGAVALGARGANYLNEGRV
                     EGEDPLAPFSPTAAAHLLRTDGFAHVADIMVNSFYDEQVDEGCAFEELISFHGGMGGP
                     QTRPFLLHPEHLPAPDEPIIGAEAAHRVLAAWRRELQGGDAAAAHASGGAAA"
     gene            complement(6487..6999)
                     /locus_tag="VFZ86_01810"
     CDS             complement(6487..6999)
                     /locus_tag="VFZ86_01810"
                     /inference="COORDINATES: protein motif:HMM:NF018590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HEX5641055.1"
                     /translation="MNDVPLDLYIAGYTDPDAAKADFDALKKAQRQGIVFIDGMVLVS
                     RDDDGRLHVKESGDHDVFKGATVGVAGGLVLGLFAPPLLAAAVVGGVVGGLIGKLAKH
                     HDEKGIAKDVDEAVPPGTSGIIAVFEEIWVDEVERQLAKAEKKALKEADKQTARELKK
                     ALAEEKKQEA"
     gene            complement(7129..7317)
                     /locus_tag="VFZ86_01815"
     CDS             complement(7129..7317)
                     /locus_tag="VFZ86_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012004089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEX5641056.1"
                     /translation="MRPVVDEVQCIGCGLCEETCPAVFEVGDDGVSHVIAPDKCVEAG
                     CCEDAADGCPVDAISLVD"
     gene            complement(7367..8041)
                     /locus_tag="VFZ86_01820"
     CDS             complement(7367..8041)
                     /locus_tag="VFZ86_01820"
                     /inference="COORDINATES: protein motif:HMM:NF022589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAP family protein"
                     /protein_id="HEX5641057.1"
                     /translation="MGSAVGEMLPLAIGIAISPLAIVAVILILTTSRARTNGPAFLGG
                     WLLGLAVVGGVALVVTDAAESADTSGPRTVVALLKIALGVALLVLAWRGFRGRPRPGE
                     EAPLPKWMAALDRFTPARSVAVGALFGGLKPKNLILTAAAAAGIAQSGLGGVQQLVVL
                     LLLVLVASIGIIAPVAIYFLKGEEKAARVLDAWKTWLQANDTTVMIVLFVVFGVVLIG
                     KGIGGL"
     gene            8275..9144
                     /locus_tag="VFZ86_01825"
     CDS             8275..9144
                     /locus_tag="VFZ86_01825"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX5641058.1"
                     /translation="MKKFVSTMVAGFLGAALLLGGAYATGIVGGSTTTQTVVKGEPAS
                     FTTSGLTPQQVYEQNVGSVVEIVATFSGGYDMWGQPTGDQQGIGTGFVVSEDGDILTN
                     AHVVSQSGVTASSVIVVFKGEGGDGVQVPATIVGSDESTDVALLRIDPSQAPELTPVK
                     LGDSSQVAVGEQVVAIGNPLGLDFSLSSGVVSATDRELQSPNGAVITGGIQTDAAINS
                     GNSGGPLFNARGEVIGINEQIASQGGGNEGIGFAVPINTAVSVMQQMQDGAFAPQTQQ
                     AAPQDDSQYYWPY"
     gene            complement(9242..10561)
                     /locus_tag="VFZ86_01830"
     CDS             complement(9242..10561)
                     /locus_tag="VFZ86_01830"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEX5641059.1"
                     /translation="MNAAPPSPAPDPQAEPGPAPATDSYPWSLYVHVIVVSVIVSFAV
                     LGWFVAYEVLNKLIWDNGYVSDHAWMFPVVCLPFSLLVGLLVKYAHAPSNLDGSILDS
                     LTGDVGQLKWKDLPATVAASLASLFSGAVLGPEGAIGNIASKVAALYCDLLRIPTDRQ
                     PKLVFASVASGYNGLLENPIFAAVLGTEVAETKRQGFSTLPAALIGGAVGYGVFLVLH
                     EAGFLGFLNLPPVQSYTLWYALLMVPLALAGLVLAMLTAAFMRIATRFFGRLEERVVL
                     RALVAGAIFSVVGVLAPVMMFSGETQVQTVIKDAADYGIALLLVMAVGKLALLSVGFR
                     SGFLGGPTFPLIFASTSVAMALNLAFPGVPVAIFVAGIMAGAVYALFRTPLMVVLLTG
                     FMLDEGATMVALIVLAVATVMIVAPPLQRLMAARQAGKKPGGTPASS"
BASE COUNT         1635 a         3659 c         3717 g         1605 t
ORIGIN      
        1 gcgtcacggc gtgctcgtgc aacgccttct cggcctcgtg gtgcgccggc tggaggtcga
       61 acgtcacctt cttgaccgtc cccgtgaacg cgtagggcgc gcggtcctcg taggcgaggt
      121 cgaccacgcc gccgttgtcg cggcccatgt ccatgccggc gtaggtcgtg aagatcagcg
      181 agatggtctt gtccagcctg ccctcgccga tctgcttgtc gtcggcccag agcgtcacct
      241 tgccgcccgc gcccggcttg ggctcgtcgg cttcgaagag catcttgacc ttcacatcgc
      301 ccgtcgggat cggctcggtc gaggtctgct tgtacgtctc cacgccgagg aactggtagg
      361 tgtggttgag cagcccattc tcgtccaccc agagcgagaa cccgccgatg aagtcggcga
      421 aggcgcagac cacgccttcg gctccgccct ccggcacgtg cagctcgccc tcgatggcgt
      481 acgagcgccc gatgatgcgc gggatcatcg tggtctggat gttctggacg tcgccggcga
      541 actccatgcg cgtgaccgtc ggcatcggtg gaagcatgcc gaagaagacc gcgtagccgc
      601 ccaggagcgg cagggcgcgg ttgcgctcgg cctcctgcca gaagagctcc ttgagctccg
      661 cgagcttctc cggattctcc gccgctacgt cgtgcgcctg gctgaagtcg tccggcaggt
      721 agtagagctc ccacgtgtcc ttttccggat cccaggtccc gcccggagcg aaccgtcgca
      781 gcgtctcggg cttgaagtcc cacggcagct tgtccagctt ggcgcaggcc caccagccgt
      841 ccttgtagat agcgcggctg ccgtacatct cgaagtactg cacggtgtgc tgctcgggcg
      901 ccagcgcgtc gtccagtgtg taggcgaagc tggtaccgtc catgggctcc tggtcgatgc
      961 catcgaccgt cgtgggctcg gggatgccgg ccagctcgag gatcgtgggc acgacgtcga
     1021 tgcagtgcgt gaactgagaa cggacggcct tgtccggcgt gatgcggccg ggccaggcca
     1081 cgaccatcgg gtcgacggtg ccgccgcggt ggctcgccat ctgcttgccc cactggaagg
     1141 gcgtgttgtt ggcgtgggcc caggcggcgg cgtagtgcgg ggcggtgtgg aagccgccca
     1201 gctcctccac gccgccgtac ttctcgatga tctccagctg ctcgtccgcc tcgagcacga
     1261 ccccgttgaa gaacgtggtc tcgttgaacg agccggtgag ggtgccctcc atgctggcgc
     1321 cgttgtcgcc ccagatgtag agaacgagcg tgttgtcgag ctcgcccatc tcctcgacgg
     1381 cgtcgagcag gcggccgacg ttccagtcgg cgttctcgga gaagcccgcg aaaacctcca
     1441 tctgtcgcgc gtacagcttc ttctgcgtcg cggtgaggga gtcccaggcg gggaagaggt
     1501 cggggcgttc agagagctcg gtgtccggag ggacgatgcc gagcttcttc tgacgctcga
     1561 gcgtctgctc gcggtagacg tcccagccgg tgtcgaactt gcccttgtac ttgtcggccc
     1621 actccttggc gacgtggtgc ggcgcgtggc tgcagccggt ggagtagtag acgaaccacg
     1681 gcttctgagg gtcgtgggcg cggaccgcat gcagccactc gaccgacttg tcggtgatgt
     1741 cgtccgggaa gtagtagagc ttgccgtcct cgccctccgg gacgccgagc gtggagttgt
     1801 cctgcgtgat gatcggatcg tactggccgg ccgcccccgt gaggaagccc caccagtggt
     1861 cgaagcccca gccctgcggc cagtggtcga acgtgcccgc cgcccccagc tcgcggcccg
     1921 gggtcatgtg ccacttgccg aacccgccgg tgacgtagcc gttctccttg aggatgcgcg
     1981 ggagggcggt acaactgcgc gggcgcgtcc ccgtatagcc ggggaacggg ccggggaact
     2041 cggcgatggc gcccatgccg acgcggtggt ggttgcggcc ggtgagcagc gcggcccggg
     2101 tcggcgagca gacggcggtg acgtggaact ggttgtaggt gagccccatg tcctgcaccc
     2161 gggtgaggtt cggcgtcgcg atgccgccgc cgaaggtgtc ggggccgccg aacccggcgt
     2221 cgtcgatgag gacgagcagg acgttcgggg cgctctcggg ggccgagaca ccggggatga
     2281 aggaccagtc ggcggccgac tcggcgatgg tgcggccaca ggccccgccg aacttcttgt
     2341 ccgggatcgg cagcacggtg cggtccgggg tgaacttgcc ggccgcctcc gcgagcgcct
     2401 ccttgaggcc tttgaggccg tgcttctcca tgggggcgac ggattttgcc ggcgagcccg
     2461 cgagcgcctt ctcggcagtc agccggtcgg cctccgcgac gatgacgatg accgccgccg
     2521 tacccggctt gagcttctcg ccgaggccgg actcgaggcc gctctccacc ttgtgcttgg
     2581 cgaacttgcc gacgatgccg ccggcggccg cgcccacggc ggtcgcggcc agaagcggcg
     2641 gcgccgccag gccgaccagg acgcccacgc cgccgcccca gcccatgccc ttgcggccga
     2701 ggtggtcgcc ggtctcggag acgcggacgt ctccgctctc gtcgtgctcg acgaggatgg
     2761 cgccctcgct cttgacctgc ttgtccttga tgagctgcac cagcccatcg aagtcgtgcc
     2821 tggccgcgtc tatggcctgg tagccggcca tcacgaccgc gaacgtctcg ctcactggtc
     2881 cctccttggc tctctcggca ggtcgcgccg gcgcaccggc ggataggcgt cttgtagggc
     2941 cgccggctca gtgagccgtg gtccagccga cctaagttac catatggtaa tggcggcgcg
     3001 cacgggatac ggttggctac gtgcgaccta gtgcgagcat gttctcacga atccggagac
     3061 acggacgtgg acaaaggcaa gcgccacagg cctagaggcg gccgggagcg gaccacggag
     3121 gccatcctcg acgccgccga gaagcttttt tcgcagcgcg gcttcgccgc cgtcagcgtg
     3181 cgggacatcg ccgccgaggc aggcgtgagc cacgcgctcg tgcaccggta cctcggctcc
     3241 aaggcgcagg tctaccggac gatgctgttg cgtcgcgaga ccgtcatccg tgacgccgcg
     3301 ccggatgtgg acgacctgat cgaggccacg aagctcatgc tgcgagaggc cgtgctccac
     3361 cagcgcgggt acgtgcgcat gctcgcctcc tcggccctcg acggcctctc gttcgagcgg
     3421 accgtcggga ggttcgcggc gaccgagcga ctcgtggaga tcgccgagaa ggtcgtggcc
     3481 ggcgaaggcg aggcccgcgg cccagacgcc cgcgaccccc gtttcgtcat cgcctccatc
     3541 gtcgccatgc tgctcggatg gtcggccgca cgggagtgga tcctgaaggc ggtcgatctc
     3601 gagccgatga gcgatgagga gttctgtgat gagttcgaga gcatcgtcct cgacctcgag
     3661 cgactgtact tccccaccct cgggggcccc gccgcggaat gacggcgggt ggcgcccgcg
     3721 tccgcggcgc gcgacagggt gaggacatgc cgcccgtggg tacaaccccg ccagacagga
     3781 gccgcgccgg ccggcgcgcg ccgggcggtc tcgatcacgg caacgaggag gatgtatgag
     3841 cgaattgatg gtgctgggct tcgagaacga ggcggccgcc gaggagttcg gcgtcacact
     3901 ggcgcagatg cagaaggaca tgatcgtgca gctgcaggac gcctccatgg tcgtgcgcga
     3961 cccggacggc aagccgcacg tcaagcacgg caacaagctc gtcggcgcgg gcgcgctggg
     4021 cggtgcgttc tggggcatgc tcttcggcct gctcttcttc gtgccgttcc tggggctggc
     4081 catcggcgcc ggcatgggcg ccctgttcgg caagctcggc aagacgggca tcgacaggca
     4141 ggtgctcgag cagatgggcg acgcggtgcc gccgggcaag gccggctggt tcctgctcat
     4201 cgggcagatg accgaggaca agttcctcgc cgccgtggag ggcacgcagg ccacgctcgt
     4261 gcgctccaac ctctcgcacg accaggaaga ggagctcaag caggccttcg gcaccgagaa
     4321 gcacgccaag gactgagcgc cggcgagacg tgagcaccac cgcgaggggc ggcccgccgg
     4381 gccgcccctc ttgcgcttcg gactacactt gtcggatccg taggacggaa ggggcctgag
     4441 gtggcagaga ccgaggcgaa ggcgtacggc gcgatccccc agtggtcgcc ggcgcgaccc
     4501 aggatccgtc cgcaccgggt cctgctccag tgggtgacga gcgccctcgc gctgtacatc
     4561 gccgcgatga tcgtgcccgg cgtcaagata gagacactcc tcggggcgtt catcgccgcg
     4621 acgttgatcg ccgtcttgaa cgccgtgctg ccgccgctca tcgccgccct ccgcctgccg
     4681 tacatgctgg ccgtcggttt tctcctggtc ctgctgctcg acgcgtggat ggtcaaggtc
     4741 gcggccgacc tgtcggacca cacgttcacc gtcgaccggt tcttctgggc tctggtggtg
     4801 gcgctggtcg cggcggccgt gacggtggcg ctcgaggtgc tgctcggcac caacgacgag
     4861 gacgtctact cgctgcgcgt cacccagcgc atcgcccgcc gctccaggga ccgcgtcgtc
     4921 accagtgtgc cgggcatcat ctacctcgag atcgacggcc tcgcccggcc cgtgctgcag
     4981 cgcgccgtcc gcgacggcca cgcgcccgag atggggcgct ggctcgcgtc cggcacccac
     5041 cggctctccg aatgggagac cgacctctcg tcgcagaccg gcgcctccca ggcggggctg
     5101 ctgctcgggt ccaacgacga catccccgcc ttccgctggg tggagaagga gtcggggcgc
     5161 atcatgacct gctcggcgcc cgcggactgc gccctcatcg aggagcggca ctcaaccggc
     5221 cgcgggctgc tggcggacgg cggcgccagc cgcagcaacc tgctctccgg cgacgccgac
     5281 cacgtgatcc tcaccgtcag caggatggag gcggagaaga aggcgaatcc cggctaccgc
     5341 gcctacctcg ccgacggcta caacgtcacg cgcacgttcg tgctcttctt ctacgaggtc
     5401 gtcctcgagc tcgccggcgc ggcgcggcag cggcgccggg acgtgcagcc gcgcggtcac
     5461 cggtctctca gctacgcgtt catgcgcgcc ggcctctccg tgttcgtgcg cgacttcacc
     5521 gtgcacagca tcatcagcga catgatgatg ggcgtgccgg cggtctacgc gtgcttcgcc
     5581 ggctacgacg aggtcgcgca ccactccggc ctcgagcgcg cggacacgat ggatgtgctg
     5641 cgcaagctcg accagcagtt cggcagggtc gcgcgcgccc gccggtacgc ggcgcgcccc
     5701 tacgagatcg tggtgctctc cgaccacggg cagacgcagg gcgccacctt caagcagcgg
     5761 aacgggtacg gcctcgacca gttggtcgaa cgcaacctgc gtgaggccgg cgtcgagaac
     5821 ctggggtccg gcgacgagaa cgacagcgcc gtgtccaagg ccgtgcgcga ggcgaccggc
     5881 cgtgagcaga aggacgagga caagcaccag gtcggcgacc gcaaggtcat cgtcatgggc
     5941 tcgggcaacc tcgggctcat ctatctgatg gacgagccga ggcgcctgac gatggaggag
     6001 atcgacgagc ggcaccccga cctgctgccg gcgctgcgcg cccacccgca cgtcggctgg
     6061 ctgctggttc gatcggccga gcacggcgcc gtggctctgg gcgcccgcgg cgccaactac
     6121 ctgaacgagg gccgcgtgga gggcgaggac ccgctggcgc cgttctcgcc cacggccgcg
     6181 gcccatctgc tgcgcaccga cggcttcgcc cacgtcgccg acatcatggt gaacagcttc
     6241 tacgatgagc aggtcgacga gggctgcgcg ttcgaggagc tcatctcctt ccacgggggc
     6301 atgggcgggc cgcagacgcg gccgttcctg ctgcaccccg agcacctgcc ggcgccggac
     6361 gagcccatca tcggtgcgga ggcggcgcac cgcgtgctgg cggcgtggcg ccgcgaactg
     6421 cagggcgggg acgcagcggc cgcgcacgcc tccggcggcg cggccgcctg agcggtcaga
     6481 gaggcctcag gcctcctgct tcttctcctc cgcgagggcc ttcttcagct cgcgggcggt
     6541 ctgcttgtcg gcctccttga gggccttctt ctcggccttg gcgagctggc gctcgacctc
     6601 gtcgacccag atctcctcga agacggcgat gatacccgag gtgcccggtg ggacggcctc
     6661 gtccacgtcc ttggcgatgc ccttctcgtc gtggtgcttg gcgagcttgc cgatgaggcc
     6721 gccgaccacg ccgccgacca ccgcggcggc caggagcgga ggggcgaaca gaccgagcac
     6781 caggccgccg gcgacgccga cggtggcgcc cttgaagacg tcgtggtcgc cggactcctt
     6841 gacgtgcagt ctgccgtcgt cgtcccggct caccaagacc atgccgtcga tgaagacgat
     6901 gccctggcgc tgagccttct tgagggcgtc gaagtcggcc ttcgcggcgt ccgggtccgt
     6961 gtagccggcg atgtagaggt cgagcggtac gtcgttcatg aggtccctcc tctgcgcgcg
     7021 tgcggcagag cacgcgtcgc cactgtggtg tcgcagctgg tcatgcccgg cgtggcgaga
     7081 agcagaaccc ccggggggag aggcgtcggc cgggggaggc gccccgccct agtcgaccag
     7141 cgagatagcg tccactgggc acccgtcggc cgcatcctcg cagcacccgg cctccacgca
     7201 cttgtcgggg gcgatcacgt gggagacgcc gtcgtccccg acttcgaaca ccgccgggca
     7261 ggtctcctca cacaggccac agccgatgca ctggacttcg tcgacgacag gtctcatggg
     7321 gcgcctcgag tggtcgcttg caggactacc cgggggtgtt cccgcgtcag agcccgccga
     7381 tgcctttgcc gatcaacacg acgccgaaga cgacgaagag cacgatcatc accgtggtgt
     7441 cgttcgcctg cagccacgtc ttccaggcgt ccaggacccg cgccgccttc tcctcgccct
     7501 tgaggaagta gatcgcgacc ggggcgatga tcccgatgct ggcgacgagg acgaggagga
     7561 gcaggacgac gagttgctgc acgccgccga ggccagactg cgcgatgccc gcggcagccg
     7621 ccgcggtgag gatgaggttc ttgggcttga gaccgccgaa cagcgcccct accgcgaccg
     7681 aacgggcggg ggtgaaacgg tccagcgccg ccatccactt cggcagcggc gcctcctcgc
     7741 ccggccgcgg ccggccgcgg aacccgcgcc acgccaggac cagcagcgcc acgccgagcg
     7801 cgatcttcag gagtgcgacc accgtgcgcg gaccgctcgt gtcggcggac tcggcggcgt
     7861 cggtcacgac gagcgcgacg ccgcccacca ccgccaggcc gagcaaccag ccgcccagga
     7921 aggccggacc gttggtcctc gcccgcgatg tggtcaggat gaggatgacg gcgacgatcg
     7981 ccagcgggct gatggcgatg ccgatcgcca gcgggagcat ctcgccgacc gcgctaccca
     8041 tgttctcctc ccggtcgccg ccggacgccg aagagcggcg actgcccgaa ccctatcgca
     8101 tgcgccggcg tcaccgtcgg gcgcgcgcac gccttccccg tgagagtctc agacgtcacc
     8161 cagacggctc tcagcggatt cccagattcg agcggtacaa ccgtggatga cacgagacgc
     8221 catccgtgag ctccgtccgg tccgacgggc accagtcatg aaaggcagcg gagaatgaag
     8281 aagttcgtca gcaccatggt cgccggcttc ctcggggcgg cccttctgtt gggcggcgct
     8341 tacgccaccg gcatcgtcgg cggctcgacc acgacccaga ccgtcgtcaa aggcgagccg
     8401 gcatcgttca ccacgagcgg gctgaccccc cagcaggtct acgagcagaa cgtcggcagc
     8461 gtcgtcgaga tcgtcgcgac gttctcgggc ggctacgaca tgtggggcca gcccaccggt
     8521 gaccagcagg gcatcggcac cggcttcgtc gtctccgagg acggtgacat cctcaccaac
     8581 gcgcacgtcg tgagccagag cggcgtgacc gccagttccg tgatcgtcgt cttcaagggc
     8641 gagggaggcg acggcgtcca ggtcccggcc accatcgtcg ggtccgacga gtccaccgac
     8701 gtcgccctgc tccgcatcga cccgagccag gcgccggagc tcacgccggt caagctcggc
     8761 gactcgtcgc aggtcgccgt gggcgagcag gtcgtcgcca tcggcaaccc gctcggcctc
     8821 gacttctcgc tctcgagcgg cgtcgtctcg gccaccgacc gcgagctgca gtcgcccaac
     8881 ggcgccgtca tcaccggcgg catccagacc gacgcggcca tcaactccgg caactccggc
     8941 ggcccgctgt tcaacgcccg cggcgaagtg atcggcatca acgagcagat cgcctcgcag
     9001 ggcggcggca acgagggcat cggcttcgcc gtcccgatca acaccgccgt cagcgtgatg
     9061 cagcagatgc aggacggcgc gttcgcgccg cagacgcagc aagcggcgcc gcaggacgac
     9121 tcgcagtact actggccgta ctgagcagcc agacaccgtc aagcacccga gggacggggc
     9181 ggccaagccg ccccgtccct ctttcgtatg ccgtcgccga ccggccggcc cgagccggcg
     9241 cctaggaaga cgctggggtc ccgccgggct tcttcccggc ctgtcgcgcc gccatgagcc
     9301 tctgcagcgg cggcgcgacg atcatcaccg tggccacggc cagcacgatc agcgcgacca
     9361 tcgtggcgcc ctcgtccagc atgaagccgg tgaggaggac caccatgagc ggggtgcgga
     9421 acagggcata gacagcccct gccatgatgc cggccacgaa gatcgccacc ggcacgccgg
     9481 ggaaggcgag gttgagcgcc atcgccaccg aggtcgaggc gaagatgagc gggaacgtgg
     9541 gcccgccgag gaacccgctg cggaacccca cgctgagcag cgcgagcttg cccacggcca
     9601 tcacgaggag cagcgcgata ccgtagtcgg cggcatcctt gatgacagtc tgcacctgcg
     9661 tctcgcccga gaacatcatg acgggcgcga ggacgccgac gacgctgaag atcgcgccgg
     9721 cgacgagcgc ccgcagcacc acgcgctcct cgaggcggcc gaagaacctg gtcgcgatgc
     9781 gcatgaaggc ggcggtcagc atggccagga cgagaccggc gagcgccagc ggcaccatca
     9841 ggagggcgta ccacagggtg tagctctgca ccggcggcag gttcaggaag cccaggaacc
     9901 cggcctcgtg caggaccagg aagacgccgt acccgacagc gccgccgatc aaggcggcgg
     9961 gcagggtgga gaacccctgc ctcttggtct cggccacctc cgtcccgagc acggcggcga
    10021 agatggggtt ctcgaggagg ccgttgtagc ccgaggcgac gctggcgaac acgagcttgg
    10081 gctgcctgtc ggtcgggatg cggagcaggt cgcagtagag ggcggccacc ttgctcgcga
    10141 tgttgccgat cgctccctcg gggccgagca ccgcgccgga gaagagtgag gcgagcgaag
    10201 cggccacggt ggccggcagg tctttccact tgagctgtcc gacgtcgccg gtcaggctgt
    10261 cgaggatcga cccgtccaga ttgctgggcg catgcgcgta cttcacgagc aggccgacga
    10321 gcagcgagaa gggcaggcag acgaccggga acatccaggc gtggtcgctc acgtagccgt
    10381 tgtcccagat cagcttgttc agcacctcgt aggccacgaa ccagccgagg acggcgaagc
    10441 tgacgatgac ggagacgacg atgacgtgga cgtacaggct ccacgggtag gagtcggtgg
    10501 cgggcgctgg tcccggctcc gcctgcgggt ccggcgcggg ggaggggggc gcggcgttca
    10561 tgagccggat agtaccctcg gctgcgtcat cctgcgcacg aaaaggggac gacgcc
//
LOCUS       DASUMX010000041         5071 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_730441, whole genome shotgun sequence.
ACCESSION   DASUMX010000041 DASUMX010000000
VERSION     DASUMX010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 5071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5071
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_730441"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="VFZ86_01835"
     CDS             <1..511
                     /locus_tag="VFZ86_01835"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEX5641060.1"
                     /translation="ATAAALARHGLACHLVPDEYVAEGLADSLAGTDAAAPGARVLIP
                     CARDARDVLPETLRERGAVVDVLPIYDTLAAEELAEPAARVEAADFITFTSASTARRL
                     AGLLQAEAAERGDPRPLAERLAGVRLCSIGPITSGALRELGLEVAVEAREYTAAGLVA
                     AIAASARSG"
     gene            complement(579..1007)
                     /locus_tag="VFZ86_01840"
     CDS             complement(579..1007)
                     /locus_tag="VFZ86_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641061.1"
                     /translation="MFGNRPEARRGRRAAGALLALLCVLVLIAAAPAVAIDHHPPTPP
                     AATVHHHPPTRSTVAFNGEATATAPRLAAVSGYARGHVPASALTTRAVVAVASFAALV
                     AAVLVALAAIAGRRTGGRTLRQIRETPTLHTSVERDRLAA"
     gene            1143..1949
                     /locus_tag="VFZ86_01845"
     CDS             1143..1949
                     /locus_tag="VFZ86_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018722030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HEX5641062.1"
                     /translation="MDRPICFFSDYGYTDDFAGVCRAAIARLAPDVRVIDVTHGLPER
                     DVLAGAVVLRNTLPYMPDKAVILAVVDPGVGGPRRPVALRSHGDRLFVGPDNGLLMHA
                     VDLDGGVDVAHELSNQELWLTPLSATFHGRDIFAPVAARLASGLPLESVGAVVAGDQL
                     DRIDLPDPVRHRDGLTAIAVLVDRFGNVALNVRARDLEKARLTETVELITGGERYHAR
                     VARTFASVRVSDIVVLIDSYGQASVAVSNGSAAEVLGLDAGAEIRIRRLG"
     gene            2103..2537
                     /gene="rplM"
                     /locus_tag="VFZ86_01850"
     CDS             2103..2537
                     /gene="rplM"
                     /locus_tag="VFZ86_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEX5641063.1"
                     /translation="MKTYVAKPSTIEKKWYVVDAKGHTLGRLATVVADCLRGKRKAIY
                     TPNIDTGDFVVIINAEQIVVTGKKPEQKMYYHHSGYPGGIKSESYAKLQARRPEEVVR
                     HAVRGMLPHNKLGRAQLRKLKIYAGPEHPHEAQNPDVLEIKE"
     gene            2541..2936
                     /gene="rpsI"
                     /locus_tag="VFZ86_01855"
     CDS             2541..2936
                     /gene="rpsI"
                     /locus_tag="VFZ86_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008538697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEX5641064.1"
                     /translation="MADAVTYRATGKRKTSVARVILTPGAGTTTINGRAIDQYFGRKV
                     LETIALMPFETTRTAGKFDVSANVYGGGISAQAGAVRHGIARALCEADESLRTPLKRA
                     GFLTRDDRQVERKKAGFHKARKKPQFSKR"
     gene            3086..3319
                     /locus_tag="VFZ86_01860"
     CDS             3086..3319
                     /locus_tag="VFZ86_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641065.1"
                     /translation="MARFKDPNRPPRNTAPLWKKVVVVLSAPVAVLVVMRLFQINTWN
                     FFLFLVVVIAIAAVAVWGMAKLLGVHLSLSSWD"
     gene            3333..4670
                     /gene="glmM"
                     /locus_tag="VFZ86_01865"
     CDS             3333..4670
                     /gene="glmM"
                     /locus_tag="VFZ86_01865"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006524003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEX5641066.1"
                     /translation="MGAYFGTDGIRGVAGELLTPEFAVQVGRAAAVVLAQKAGPHPLF
                     VIGRDTRLSGPMLEAALTAGLTSGGGRVEIAGVIPTPGLAALVQQRGADAGIVISASH
                     NPFADNGIKFFSGAGVKLSDEEEAEIEAHIEHHDLQSLAGADFGSAEQLEGAVEGYVR
                     DVVERIPLDLSGMRIALDCAHGAMHLAAPLALEESGAAVTVLFDRPDGTNINAGCGST
                     HIGALQKVVADGGFDLGLAFDGDGDRVLAVDAAGDLVDGDFLIAILAKHLKAQGRLRS
                     DTVVTTVMTNLGFHLAMKREGIEVLVTAVGDRYVVAEMLKGDYLLGGEQSGHIINREI
                     STTGDGLATSLLLLQALRQMDVPLAEAARVMERLPQKLVNVRVRDLAELEGATAVWAA
                     VEQETRRLEGSGRVLVRPSGTEPLVRVMAEAPTQDEVDATCGRIVAVVEHYLT"
     gene            4685..>5071
                     /locus_tag="VFZ86_01870"
     CDS             4685..>5071
                     /locus_tag="VFZ86_01870"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX5641067.1"
                     /translation="MSDEVKYYPFRIGGVEQVHPNVHPGTPKYAAGIVVGNIAFLSGM
                     TPQSTEDGSCLTVGVEAQVFACLDKVRAVLEEMGSTMENIVKTVILLKDLEYYQQMRA
                     AELKYYQQYAPRLVSEPPASTYVQPAI"
BASE COUNT          741 a         1873 c         1755 g          702 t
ORIGIN      
        1 ggccaccgcc gcggcgctgg cgcgccacgg cctcgcctgc cacctcgtcc ccgacgagta
       61 cgtcgccgag ggcctggcgg actcgctggc ggggacggac gccgccgcgc cgggcgcccg
      121 cgtgctcatc ccctgcgccc gcgacgcccg cgacgtgctc cccgagacgt tgcgcgagcg
      181 cggcgccgtg gtcgacgtac tgcccatcta cgacacgctc gccgccgagg agctcgccga
      241 gccggcggcg cgggtggagg cggcggactt catcacgttc acctccgcca gcaccgcccg
      301 gcggctggcc gggctgctgc aggcggaggc ggccgagcgc ggcgacccgc ggcctctcgc
      361 cgagcggctc gccggcgtcc gcctctgctc catcggcccg atcacgagcg gggctctgcg
      421 tgagctcggc ctcgaggtcg cggtcgaggc ccgggagtac acggccgcgg gcctcgtcgc
      481 ggcgatcgcc gcctctgcgc ggagcggctg agccgcaggc tctcgcccgg gcgccggctg
      541 cgatgccggc gcggctgtcg cactcccgag ggcagtcgtc aggccgcgag ccgatcccgc
      601 tcgacgctcg tgtgcagagt gggcgtctcg cgaatctggc gcagcgtccg gccacccgtc
      661 cgcctcccgg cgatggcggc cagcgccacc aggaccgcgg caacgagggc cgcgaacgac
      721 gcgacggcca ccacggcgcg ggtcgtgagc gcagacgccg gaacgtggcc gcgtgcatag
      781 ccgctgacgg cagccagcct cggcgccgtt gcggtcgcct ccccgttgaa cgccactgtg
      841 gaccgtgtcg gggggtgatg gtggactgtc gccgccggcg gcgtcggcgg atgatggtcg
      901 atcgccactg ccggagcggc cgcgatcagc accaggacac acagaagtgc gagcagcgcc
      961 ccggccgccc ggcgccccct ccttgcttct ggacgattgc cgaacatgtc agctcccttc
     1021 tgcgtagtgg tgtcagggcg gactctacgc aggtcagccc cggcgggcgt cacccttccg
     1081 gggtgatatc gaccgcacgc tcaactgttg cgccgggcca ggcttcgtca taggttcgcg
     1141 gcatggaccg accgatctgc ttcttcagcg actacgggta cacggacgac ttcgccggcg
     1201 tgtgccgtgc cgccatcgcc cggctggcgc ccgacgtgcg ggtcatcgac gtcacccacg
     1261 gcctgcccga acgcgacgtg ctggccggcg ccgtggtgct ccgcaacacg ttgccctaca
     1321 tgccggacaa ggccgtgatc ctcgcggtcg tcgacccggg cgtgggcggg ccgcggcggc
     1381 cggtcgccct gcgcagccac ggcgaccggc tcttcgtggg cccggacaac gggctgctca
     1441 tgcacgccgt cgacctcgac ggcggcgtcg acgtcgccca cgagctgagc aaccaggagc
     1501 tgtggctgac cccgctctcg gccaccttcc acggccgcga catcttcgct ccggtcgccg
     1561 cgcggctcgc ctccgggctg ccgctcgagt cggtcggggc ggtggtcgcc ggcgaccagc
     1621 tcgaccgcat cgacctgccc gaccccgtgc ggcaccgcga cggcctcacc gccatcgcgg
     1681 tgctcgtcga ccgcttcggc aacgtcgccc tcaacgtgcg cgcccgcgac ctcgagaagg
     1741 cgcgtctcac cgagaccgtc gagctcatca ccggcggtga gcgctaccac gcgagggtgg
     1801 cgcgcacctt cgcgtcggtg cgcgtcagcg acatcgtcgt gctcatcgac agctacggcc
     1861 aggcgtcggt ggccgtgagc aacggctcag ccgccgaggt gctcggcctc gacgccggcg
     1921 ccgagatccg catccggcgc ctcggctgac ctcgggcacg cccggccgga cggtacgggc
     1981 gccgtcctgc cgccacggcg cggcctcacg gcgcccgtgt tgcgccgccc ctccggattc
     2041 gctatagtcc cctttcgcgc gcccgagccg ggcgcgctcc ctgctaccga acgagtgagc
     2101 gcatgaagac ttacgtcgcc aagccttcca ccatcgagaa gaagtggtac gtcgtcgacg
     2161 ccaagggcca caccctgggc cgcctcgcga ccgtcgtcgc cgactgcctg cgcggcaagc
     2221 gcaaggccat ctacaccccc aacatcgaca ccggcgactt cgtcgtcatc atcaacgccg
     2281 agcagatcgt cgtgaccggc aagaagcccg agcagaagat gtactaccac cactcgggct
     2341 atcccggcgg catcaagagc gagtcgtacg ccaagctcca ggctcgccgc cccgaggagg
     2401 tcgtccgtca cgccgtgcgc ggcatgctgc cccacaacaa gctcgggcgc gcccagctcc
     2461 gcaagctcaa gatctacgcc gggccggagc atccgcacga ggcgcagaat cccgacgtgc
     2521 tcgagatcaa ggagtaaggc gtggctgatg cagtgaccta tcgcgcgacc ggcaagcgca
     2581 agaccagcgt cgcgcgcgtc atcctcacgc ccggcgccgg cacgacgacc atcaacggcc
     2641 gtgcgatcga ccagtacttc ggccgcaagg tgctcgagac catcgccctc atgccgttcg
     2701 agaccacgcg cacggccggc aagttcgacg tctcggccaa cgtgtacggc ggcggcatca
     2761 gcgcccaggc cggcgctgtg cgccacggca tcgcgcgcgc gctctgcgag gccgacgaga
     2821 gcctgcgcac gccgctcaag cgcgccgggt tcctcacccg cgacgaccgt caggtcgagc
     2881 gcaagaaggc cggcttccac aaggcgcgca agaagccgca gttcagcaag cgctgaccct
     2941 gcgcccagat ccgcgtttcc cgacgggccg ccgcgaggcg gcccgtcgtc gttccggacg
     3001 gcggcgctgc gccgtcctgc agccgcctcc ccgcctcctc cgcgggtgct aggatggccc
     3061 gagtcaaatc gcgaggagtc ctctgatggc ccgcttcaaa gacccgaacc gcccgccgcg
     3121 gaacaccgcc cccttgtgga agaaggtcgt cgtcgtgctc tccgcgccgg tggccgtgct
     3181 cgtcgtcatg aggctgttcc agatcaacac gtggaacttc ttcctgttcc tcgtcgtcgt
     3241 catcgccatc gcggccgtgg ccgtgtgggg gatggccaag ctgctcggcg tgcacctcag
     3301 cctcagcagc tgggattaag gatctccgcg gcatgggcgc gtatttcggc acggacggca
     3361 tccggggggt cgcgggagag ctgctgaccc ccgagttcgc cgtgcaggtc gggcgcgccg
     3421 cggccgtggt gctggcgcag aaggccggcc cgcacccgct gttcgtcatc ggccgtgaca
     3481 cgcgcctcag cggcccgatg ctcgaggcgg cgctcacggc cggcctcacg agcggcggcg
     3541 gccgtgtgga gatcgccggc gtgatcccga cgccggggct cgccgcgctg gtccagcagc
     3601 gcggcgccga cgccggcatc gtcatcagcg cctcgcacaa tcccttcgcc gacaacggca
     3661 tcaagttctt ctcgggcgcc ggcgtgaagc tgagcgacga ggaggaggcc gagatcgagg
     3721 cgcacatcga gcaccacgac ctgcagtcgc tggcgggcgc cgacttcggc agcgccgagc
     3781 agctcgaggg cgccgtggag ggctacgtcc gggacgtcgt cgagcggatc cccctggatc
     3841 tcagcggcat gaggatcgcg ctcgactgcg cgcacggcgc gatgcacctg gccgcgccgc
     3901 tggcgctgga ggagtccggc gcggcggtca ccgtgctctt cgaccggccg gacggcacca
     3961 acatcaacgc cggctgcggg agcacgcaca tcggcgcgct gcagaaggtc gtggccgatg
     4021 gcggcttcga cctcgggctc gcgttcgacg gcgacggcga ccgcgtgctc gccgtcgacg
     4081 ccgccggcga cctcgtggac ggcgacttcc tcatcgccat cctcgcgaag cacctgaagg
     4141 cccagggcag gctccgcagc gacaccgtgg tgaccacggt catgaccaac ctcggcttcc
     4201 acctcgccat gaagcgggag ggcatcgagg tgctcgtgac cgccgtgggc gaccgctacg
     4261 tcgtcgcgga gatgctcaag ggcgactacc tgctcggcgg ggagcagtcc gggcacatca
     4321 tcaaccgcga gatcagcacc accggcgacg gcctcgccac gtcgctcctg ctcctgcagg
     4381 cgctgcggca gatggatgtg ccgctggccg aggcggcgag ggtgatggag cgcctgccgc
     4441 agaagctcgt caacgtgcgc gtgcgcgacc tggccgagct cgagggagcc accgcggtgt
     4501 gggcggcggt ggagcaggag actcgccgcc tcgagggcag cggccgcgtg ctcgtgcggc
     4561 cgtcgggtac cgagccgctc gtgcgcgtca tggccgaggc gcccacccag gacgaggtcg
     4621 acgcgacctg cggccgcatc gtggcggtcg tcgagcacta tctgacctga cgaaggggat
     4681 cgcagtgagc gatgaagtga agtactaccc gttcaggatc ggcggtgtcg agcaggtcca
     4741 tcccaacgtg catccgggta cgcccaagta cgcggcaggc atcgtggtgg gcaacatcgc
     4801 cttcctctcg ggcatgactc cccagagcac cgaggacggg tcgtgtctca ccgtcggggt
     4861 ggaggcccag gtgttcgcct gcctcgacaa ggtgcgcgcc gtgctcgagg agatgggcag
     4921 cacgatggag aacatcgtga agaccgtcat cctgctcaag gacctcgagt actaccagca
     4981 gatgcgcgcc gccgagctca agtactacca gcagtacgcg ccgcgcctcg tctcggagcc
     5041 gccggccagc acctacgtgc agccggcgat c
//
LOCUS       DASUMX010000042        11715 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_299391, whole genome shotgun sequence.
ACCESSION   DASUMX010000042 DASUMX010000000
VERSION     DASUMX010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 11715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11715
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299391"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="VFZ86_01875"
     CDS             complement(<1..469)
                     /locus_tag="VFZ86_01875"
                     /inference="COORDINATES: protein motif:HMM:NF012998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HEX5641068.1"
                     /translation="MTSGSDRVAFLGPEGTFTEEALLANMPEGGLHPFPYPSIRDVLR
                     AVQGGEVPLGIIAIENALEGSVPVTLDSFASGFDDLQIVREVTHPVHQMLITRREMPL
                     DRITKVISIPHAYGQCRDFIREHLGNVEHEATDSTAEAVRRVSRVDRPWAAVGT"
     gene            complement(545..1321)
                     /locus_tag="VFZ86_01880"
     CDS             complement(545..1321)
                     /locus_tag="VFZ86_01880"
                     /inference="COORDINATES: protein motif:HMM:NF025935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4446 family protein"
                     /protein_id="HEX5641069.1"
                     /translation="METLSDIAPYVALGAAALALVLLVLVIVLLARVGKLRRAQTVVL
                     GHHEQRDIVAHTEDLDAQVRNLREAVEILTDQLDDQKRHLDRALTNRAIIRYDAFRDA
                     GGEQSASFALLDQYRSGVVFSAIAARDFARIYVKHITEGVADRDLSPEEAKVMEAAVP
                     RPLKSGEEGPRASRGPRPESLPPAGLREPAPERQPAEDAAPSAEATAAFEALGEAVGP
                     SGPAEAAGAPPGDEGVAPDGADDFTWDPPPGRPRGGDSRR"
     gene            complement(1325..2428)
                     /locus_tag="VFZ86_01885"
     CDS             complement(1325..2428)
                     /locus_tag="VFZ86_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641070.1"
                     /translation="MDFFDDESSRAPADAEGSPPAPPSRRRPDRRRTRLQRIAILALV
                     LFAIVFAAAWWARSCQHNRKVDSYRTYFEGVGVAIKDSEALGKQVSAIMQDPTSLSRK
                     ELIDKLEQLTAQQEEIAVRAGRLEPPGTLEDEQTVFSTGMKVRASGFRLLRTAMLGAL
                     DNKKVGVKQITALDGYFSGPDAYYQELVYLPARETMAEEGVSDVAVPTSTYYLAWNAL
                     DPVTVLVALESVGKSSKLSGRHGVALIDVTAQGSSGDLKLVPGRSNDVPASPDLSFVI
                     QVQNQGSVIEKNVRVTATLTLPGGAANTIKQQATIASIESGKTQDVTITGFAIPTDAL
                     SKEVTLKVVAGPVKNEFDPSNNSATYKLTLQLK"
     gene            complement(2456..2542)
                     /locus_tag="VFZ86_01890"
     tRNA            complement(2456..2542)
                     /locus_tag="VFZ86_01890"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2506..2508),aa:Leu,seq:cag)
     gene            2614..4329
                     /locus_tag="VFZ86_01895"
     CDS             2614..4329
                     /locus_tag="VFZ86_01895"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5641071.1"
                     /translation="MSVAAQSPAPYYENWRDRYQLVGKIGSGGFAEVFEAYDLTLEEP
                     VALKIVPDGRALSARIVREVEAAASLDHPNIVALHDWFADDEGSILVWELVRGDSLDK
                     LGHQLDDGDVVAIGVELLDALAYAHSQGIVHRDVKPQNVMLDDGGHVKVMDFGIAHLM
                     DSDTLTGDGDVIGTIAYMSPEQAAGRRVQPPSDVYSAGMVLYELLAGAHPLRGDTPAE
                     TLSNVAAARLPSLATLRPDLPGELVTLIDAACAPRPAERPTPSYLSEALDDLLRCGTL
                     QTRRLRKAQSLLRPLGRAAEVAERAGGAALAAVTSAVTLGALPAYPQSWTLPLVALTA
                     AVWAVVPQAGLAFLLGSLAFPLFNVSLSLGGAYLAFAVAVFLVARTRPITALWPAFAL
                     VLMPAYLTLLAPAAAALLGRVRGPLTAAWAGAGTVVFLLLVRAPRGPFTLEQPRWHVA
                     DGLAAAGDPFTVAGRVLVHALAAPTLLQAAVWAGLAAALGHALTRRRLETRLWIWSLS
                     FAAVFAVYRIAPVAVWDRPASLWPLVWSVAVPAAVILLPLVLTTGQAPEEACDGDLQE
                     GEQEA"
     gene            4295..5098
                     /locus_tag="VFZ86_01900"
     CDS             4295..5098
                     /locus_tag="VFZ86_01900"
                     /inference="COORDINATES: protein motif:HMM:NF023818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3662 and FHA domain-containing protein"
                     /protein_id="HEX5641072.1"
                     /translation="MGIFKKANKKLEGFVEGGFGRAFKSSVQPVELAHKLAKEMGDHK
                     TVGVSNVYVPNEFDVYLGKDDYDHLVSFEDSLYTELSNYVVAFARREGWTLVAPPRIE
                     LHADDDLRVGEFGIATRTVSAPAEAPGAGAFAAPAAAVPPAAAIAPGPVIDQTVLYQE
                     PPAAAAAAAAPRASSARGVLRGRQGDYQLADAVTVIGRSRRCDIVLTDANVSRQHAEI
                     RRQDSGFMLLDLGSTNGTRVNRRDVKQVVLQHGDRIELGTTEFLFERQP"
     gene            5162..5707
                     /locus_tag="VFZ86_01905"
     CDS             5162..5707
                     /locus_tag="VFZ86_01905"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX5641073.1"
                     /translation="MIDIVLLVLKIGILVLLYLFIWQVVKTAVQNVRGGGGAAAPAAD
                     VVLPAGDRLAPVFTPAEREQRREDRLVERTQDGQKMDFSAHINPRLIVEESPIIPPGV
                     IFPLEGWITVGRAPASDIVLDEQFVSTTHARLLPRGQFYYVEDLGSTNGTFVNEKQVT
                     EAQLKLDSRVRIGETTFRYEE"
     gene            5715..6938
                     /locus_tag="VFZ86_01910"
     CDS             5715..6938
                     /locus_tag="VFZ86_01910"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HEX5641074.1"
                     /translation="MTLVARHGALTDIGLHRKTNEDTFVVRPPLYAVCDGMGGASAGE
                     VASGLAAETLAAEVAGGTPLHAAAEAANAAVFRRAHDNLEQTGMGTTLTAFVLEGGTA
                     RFAHIGDSRAYLLRDGELRQVSDDHSLVGEMMRDGRLTEEEAAVHPHRSILSRALGTE
                     PQARIDEFTEDLLPGDVLLLCSDGLSGPVSADAMRVALTHSDPQAAAERLILEARRAG
                     GPDNITAVVVRLVEGPHGAEDDETTLVQAVDPEADVATGELPFVVAAPGGGEPAGDAA
                     DALAAEAEALRAEEAGSTVAADVAAAAARRRRRSVRRLGIVAAALGLLTAALAAGAFF
                     LSTMFFIGVDDGTLTVYSGAPVKIGPVPLHAVYRRSHVAYESLSPSARSLVDEQQLRD
                     RQDALDLGGLLGMWR"
     gene            6935..8257
                     /locus_tag="VFZ86_01915"
     CDS             6935..8257
                     /locus_tag="VFZ86_01915"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEX5641075.1"
                     /translation="MSRRNLELVFLVLAGAVSTMAYVSVYAGRFREINRISIIYGLVF
                     VAIFVLLHLVERIFLPQADSFLLPITALLAAIGLTEIFRIKPALALTQGQWLLVGAGL
                     FVLTVVIVRDHVKLDRYRYLIGAVGLALLVITILAGTTINGAKLWIHAFGFSIQPSEF
                     AKLAIVIFLAGYLDDKKVMLSVPQRHVLGVPVPAFKYFGPLLVMWVLSLAMLVFMKDF
                     GMALLFMSIFVGLMYMATARVVYVVAGIGLFAAAGAIALAIVPHVQDRFTVWLDPWPY
                     AATTGYQLLQSLFTIADGGVVGAGFGRGYLLFADRQPVVPDLQTDFIFTAIANEMGLL
                     GAAGVVLVYLLFVWRGFRIAVFAPDGFSKLLAAGLATAFALQTFIIIGGVTRLIPLTG
                     ITLPFVSYGGSSITANLMLVALLLMVSNRSNIIRGGTELERRRLAERG"
     gene            8262..9701
                     /locus_tag="VFZ86_01920"
     CDS             8262..9701
                     /locus_tag="VFZ86_01920"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX5641076.1"
                     /translation="MNRAMSKAFVITVVLFVALIANLTWIMVVRAQWFQDRPENKRSI
                     AKEMKIKRGDILGYDGSVIAGTERRSGYYHRRYPSGTLAPQLLGYDSVRYGRSGIEAQ
                     LNDELTGQSSDLGVQNWVDKLLGRRPEGADVRLTLVPAVQKVAQSALKGQKGAIVVLD
                     PTTGALIASASAPTYDPANLEDRWARLRKDASAPLLNRPTQGLYVPGSAFKVVTASGG
                     LDTGRVTPDTEFVDTGTYVVFGGKVTNYGGAVYGPNDFTTALTLSINTTFAKVGNLLG
                     RKRLIAAAERYGFYQKPPLELPAGEVVASGRYGKKGLLDPDAFMDPLDVAWAACGQER
                     LLATPLQMALVAGGVANGGRVMKPYYLQEVVTPDGRVVRTTQPEQWLAATKALTAGEL
                     NLMMQRVVNSGTGTAAALEGIQVAGKTGTAEAGGGSNLAWFIGFAPADDPQVAIAVVI
                     EDTQSTGGEAAAPLAAAVIKAALAQPALP"
     gene            9763..11643
                     /gene="pknB"
                     /locus_tag="VFZ86_01925"
     CDS             9763..11643
                     /gene="pknB"
                     /locus_tag="VFZ86_01925"
                     /inference="COORDINATES: protein motif:HMM:NF033483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEX5641077.1"
                     /translation="MSDVFQPEQVIGGRYRVVRKLGGGGMADVYLCEDLTLGRHVAIK
                     VLLQRYLNDATFVERFRREAKAAAGLNQQNLVAIYDWGEVDGTYYIAMEYVEGETLKD
                     LIRRRGRLSGNESVAVAMQLLAAVEFAHRSGIVHRDIKPQNVMLDRGGTVKVMDFGIA
                     RAGDSGMTEAGSILGTAQYLAPEQAMGHPVDERSDLYSVGVVLYEMLTGTVPFKGDSA
                     VTVALKHVNEVPREPSELVPGMPYALNQIVLKAMAKDPADRYQSAAEFARDLRAAREG
                     GPVQAAAFDAGGERTRVMTGAAGMTAAEATSVLDQPLPPRRKKSRWPLILIVLLLAII
                     AAVAVALWWTMSGNKAEVPGVVGLSRAAAVRALEQDGFKAAVQQEFSERVAEGFVSRQ
                     APTAGTKLREGATVDIWVSKGSQKAEPLPDFSGWTPSMVDDWLARNDLQGDPRTGRSN
                     AVSEGQVFRQDPAPGTQLNRGDTVSYWVSRGKPQATVPDLTNLTQADAEAALAEAGLR
                     LGSVTPETSETVPAGAVVRQDPPAGEKVDKDSVVNIVVSSGPPTPSPSPTVSPSGVAV
                     PNVYGMQSSIAEQELVALGLAAAFREKPNTGQQPGTVVSVKPDAGTVVPAGSTVTLVI
                     AS"
BASE COUNT         1620 a         4220 c         4152 g         1723 t
ORIGIN      
        1 gcgtgcccac ggccgcccag ggccggtcca cgcggctcac ccggcgcacg gcctccgcgg
       61 tcgagtcggt cgcctcgtgc tccacgttgc cgaggtgctc gcggatgaag tcgcggcact
      121 ggccgtaggc gtgcgggatg ctgatcacct tggtgatccg gtcgagcggc atctcccgtc
      181 ttgtgatgag catctggtgc acggggtgcg tgacctcgcg cacgatctgc aggtcgtcga
      241 agcccgaggc gaagctgtcc agcgtgacgg ggacggatcc ctcgagcgcg ttctcgatgg
      301 cgatgatgcc gaggggcacc tcgccgccct ggacggccct gagcacgtcg cggatggacg
      361 gatagggaaa cgggtgcaga ccgccctccg gcatgttggc gagcagcgcc tcttcggtga
      421 acgtgccctc cgggcccaga aaggccactc tgtcgctgcc gctcgtcacg ccggcgtctc
      481 ctccgtccgc tgctgcgctc ctacccgcta ggctatcaga ccacgcgggc ccgtttgcgg
      541 aggctcagcg gcgggagtcg ccgccgcgcg ggcggccggg cggcgggtcc caggtgaagt
      601 cgtcggcgcc gtccggagcc acgccctcgt ccccgggcgg tgcgcctgcg gcctcggccg
      661 gcccggaggg gccgacggcc tcgcccagcg cctcgaacgc cgccgtcgcc tccgccgagg
      721 gcgcggcgtc ctccgccggc tggcgctcgg gcgccggctc gcggagcccc gccggcggca
      781 ggctctcggg ccgcggcccg cggctggccc gcggcccttc ttcgccgctc ttcagcggcc
      841 gcggcaccgc ggcctccatc accttggcct cttccggcga gaggtcgcgg tcggccacgc
      901 cctcggtgat gtgcttgacg tagatgcgcg cgaagtcccg cgccgcgatg gcgctgaaga
      961 ccacgcccga ccggtactgg tcgagcaggg cgaacgaggc gctctgctcg ccgccggcgt
     1021 ccctgaaggc gtcgtagcgg atgatcgcgc ggttggtgag cgcgcggtcg aggtggcgtt
     1081 tctggtcgtc gagctggtcg gtgaggatct ccaccgcctc gcgcaggttg cgcacctgcg
     1141 cgtcgagatc ctcggtgtgc gcgacgatgt cgcgctgctc gtggtgcccg agcacgaccg
     1201 tctgcgcgcg ccgcagcttg cccacgcgcg cgaggagcac gatcacgagg acgaggagga
     1261 cgagcgcaag agccgcggcc cccagcgcta cgtacggggc gatgtcgctc agggtctcca
     1321 tcgcctactt gagctgcagg gtgagcttgt aggtggcgga gttgttgctc gggtcgaact
     1381 cgttcttgac cggcccggcg accaccttga gcgtgacctc cttgctcaag gcgtcggtgg
     1441 ggatggcgaa cccggtgatg gtgacgtcct gcgtcttgcc ggactcgatg ctggcgatgg
     1501 tcgcctgctg cttgatggtg ttggcggcgc cgccgggcag ggtgagcgtg gccgtgacgc
     1561 gcacgttctt ctctatgacg ctgccctggt tctggacctg gatgacgaag gagaggtcgg
     1621 gcgaggccgg cacgtcgttg ctgcgcccgg ggacgagctt gaggtcgccc gagctgccct
     1681 gggcggtgac gtcgatgagg gcgacgccgt ggcggccgga gagcttcgac gacttgccca
     1741 cgctctcgag cgccacaagc acggtgaccg ggtcgagggc gttccacgcg aggtaatagg
     1801 tggaggtggg cacggcgacg tcgctgacgc cctcctccgc cattgtctcc ctcgcgggca
     1861 ggtagacgag ctcctggtag taggcgtccg ggccgctgaa gtagccgtcg agagcggtga
     1921 tctgcttgac gccgaccttc ttgttgtcga gtgccccgag catggccgtg cgcagcaggc
     1981 ggaagccgct ggcgcggacc ttcatgccgg tcgagaacac ggtctgctcg tcctcgagcg
     2041 tgcccggcgg ctcgagccgg ccggcgcgca cggcgatctc ctcctgctgg gcggtgagct
     2101 gctcgagctt gtcgatgagc tccttgcgcg agaggctcgt ggggtcttgc atgatggccg
     2161 agacctgctt gcccagggcc tcggagtcct tgatcgccac gccgacgccc tcgaagtagg
     2221 tgcggtagct gtcgaccttg cggttgtgct ggcagctgcg cgcccaccag gcggcggcga
     2281 acacgatcgc gaacaggacc agcgccagga tcgcgatgcg ctggaggcgg gtgcggcggc
     2341 ggtcggggcg gcggcgggac gggggagcgg gtggcgagcc ctcggcatcg gcgggcgcgc
     2401 ggctgctctc gtcgtcgaag aaatccaccc cgcggaaccc ccgtatctag gtggatggtg
     2461 ggcgaggggg gacttgaacc cccacgagcg taagctcact agaccctgaa cctagcgcgt
     2521 ctgccaattc cgccactcgc ccatcgggcc tgacagggtt cggattgtac cacaccgaat
     2581 atcgaggaaa ccgaggccga aacgagtccc cgaatgtccg tcgccgcgca gagccccgcc
     2641 ccctattacg agaactggcg cgaccgctac cagctggtcg gcaagatcgg cagcggcggg
     2701 ttcgccgagg tcttcgaggc ctacgacctg acgctggagg agcccgtcgc gctcaagatc
     2761 gtccccgacg ggcgggcgct cagcgcgcgc atcgtgcgcg aggtcgaggc ggccgcgtcg
     2821 ctcgaccacc ccaacatcgt cgcgctccac gactggttcg cggacgatga gggcagcatc
     2881 ctcgtctggg agctcgtccg cggcgactcg ctggacaagc tgggccacca gctggacgac
     2941 ggggatgtgg tcgcgatcgg cgttgagctc ctcgacgcgc tcgcgtacgc gcacagccag
     3001 ggcatcgtcc accgcgacgt caagccccag aacgtcatgc tcgacgacgg ggggcacgtc
     3061 aaggtgatgg acttcggcat cgcccacctc atggactcgg acacgctcac cggcgacggc
     3121 gacgtgatcg gcaccatcgc ctacatgtcg ccggagcagg cggccgggcg ccgcgtccag
     3181 ccgcccagcg acgtctactc cgccggcatg gtgctgtacg agctgctggc cggggctcat
     3241 ccgctgcgcg gggacacccc ggcggagacc ctctccaacg tcgccgccgc gcgcctgccg
     3301 tcgctggcga cgctgcgccc ggacctgccc ggcgagctcg tcacgctgat cgacgcggcc
     3361 tgcgcgccgc ggccggccga gcgcccgacg ccgtcctacc tgagcgaggc gctggacgac
     3421 ctcctgcgct gcgggacctt gcagacgcgg cgcctccgga aggcccagag cctcctccgg
     3481 ccgctcggcc gcgccgccga ggtcgcggag cgcgccggcg gcgcggcgct ggccgccgtc
     3541 accagcgccg tgacgctcgg cgcgctgccg gcgtacccgc aaagctggac gctccccctc
     3601 gtcgcgctga cggccgccgt ctgggccgtg gtgccccagg caggtctcgc gttcctgctc
     3661 ggctcgctgg ccttcccgct gttcaacgtc tcgctgagcc tggggggcgc gtacctcgcg
     3721 ttcgccgtgg cggtgttcct ggtcgcgcgt acgaggccca tcacggcgct gtggccggcg
     3781 ttcgccctgg tgctcatgcc ggcctacctc acgctgctgg cgccggcggc cgcggcgctg
     3841 ctgggccgcg tccgcggccc gctcaccgcc gcgtgggccg gcgccgggac ggtcgtcttc
     3901 ctgctgctgg tgcgcgcgcc tcgcggcccg ttcacgctcg agcagccgcg gtggcatgtc
     3961 gccgacggcc tcgcggcggc cggcgacccg ttcaccgtcg ccggccgcgt gctggtgcac
     4021 gcgctcgccg cgccgacact gctgcaggcc gccgtctggg ccggtctggc ggccgcgctc
     4081 ggtcacgcgc tgacgcgccg ccgcctcgag acccgcctgt ggatctggtc gctttccttc
     4141 gccgccgtgt tcgccgtgta ccgcatcgcg ccggtcgccg tctgggaccg ccctgcgagc
     4201 ctgtggccgc tcgtctggag tgtggccgtg cccgccgccg tgatactctt gccgctggtc
     4261 ctgaccaccg gacaagcgcc ggaggaagcc tgcgatgggg atcttcaaga aggcgaacaa
     4321 gaagcttgag ggcttcgtcg agggcgggtt cgggcgcgcg ttcaagtcga gcgtgcagcc
     4381 ggtcgagctc gcgcacaagc tggccaagga gatgggcgac cacaagacgg tcggcgtctc
     4441 caacgtctac gtgcccaacg agttcgacgt gtacctgggc aaggacgact acgaccacct
     4501 ggtctccttc gaggactccc tctacacgga gctgtccaac tacgtggtcg cgttcgcgcg
     4561 gcgcgagggc tggacgctcg tcgcgccgcc gcgcatcgag ctgcacgcgg acgacgacct
     4621 gcgcgtcggc gagttcggca tcgcgacgcg caccgtgagc gcgccggccg aggcgcccgg
     4681 cgccggcgcg ttcgccgctc ccgctgcggc cgtgcctccc gccgcggcca tcgcgcccgg
     4741 cccggtgatc gaccagacgg tgctctacca ggagcctccg gccgcggccg cggctgcagc
     4801 cgctccgcgt gcctcttcgg cgcgcggcgt cctgcgcggc cggcagggcg actaccagct
     4861 cgccgacgcc gtcaccgtca tcggccgcag ccgccgctgc gacatcgtcc tcacggacgc
     4921 caacgtctcg cggcagcacg cggagatccg ccgccaggac agcggcttca tgctgctcga
     4981 cctcggctcc acgaacggca cacgcgtcaa ccggcgcgac gtcaagcagg tcgtgctgca
     5041 gcacggcgac cgcatcgagc tcggcaccac cgagttcctg ttcgagagac agccctgacc
     5101 gggaggcccc ggtgaccacg gcgccggccg gcccgacggg cgggcggcgc gggagagtcg
     5161 catgatcgac atcgtcctgc tcgtcctcaa gatcggcatc ctcgtcctgc tctacctgtt
     5221 catctggcag gtcgtgaaga ccgccgtgca gaacgtgcgc ggcggcggtg gcgccgccgc
     5281 tcccgccgcc gacgtggtcc tgccggccgg cgaccgtctg gcgcccgtgt tcacgccggc
     5341 cgagcgcgag cagcgccgcg aggaccgcct ggtcgagcgt acccaggacg gccagaagat
     5401 ggacttctcc gcgcacatca acccgcgtct catcgtcgag gagagcccca tcatcccgcc
     5461 aggggtgatc ttcccgctcg agggctggat cacggtcggc cgcgcccccg ccagcgacat
     5521 cgtgctcgac gagcagttcg tctccaccac gcacgcgcgc ctgctgcccc gcggccagtt
     5581 ctactacgtc gaggacctcg gcagcaccaa cggcaccttc gtcaacgaga agcaggtgac
     5641 cgaggcgcag ctcaagctgg acagccgggt acgcatcgga gagaccacgt tccgctacga
     5701 ggagtgagcg ccgggtgacg ctcgtcgcca gacacggcgc cctcaccgac atcgggctcc
     5761 accgcaagac gaacgaggac acgttcgtcg tgcggccgcc gctgtacgcc gtctgcgacg
     5821 gcatgggcgg cgccagcgcc ggagaagtcg ccagcggtct ggccgcggag acgctggccg
     5881 ccgaggtcgc cgggggcacg ccgctgcacg cggccgccga agcggccaac gcggcggtgt
     5941 tccggcgcgc ccacgacaac ctcgagcaga cggggatggg caccacgctc acggcgttcg
     6001 tgctcgaggg cggcacggcg cgcttcgccc acatcggcga cagccgcgcc tacctgctcc
     6061 gcgacggcga gctgcgccag gtcagcgacg atcactcgct ggtcggcgag atgatgcgcg
     6121 acggccgcct gaccgaagaa gaggcggccg tccatccgca ccgcagcatc ctgagtcgcg
     6181 cgctcggcac cgagccgcag gcgcgcatcg acgagttcac cgaggacctg ctgccgggcg
     6241 acgtgctgct gctctgcagc gacgggctga gcggaccggt gtctgcagac gccatgcgcg
     6301 tcgcactcac gcacagcgac ccccaggccg ctgccgagcg cctcatcctc gaggctcgtc
     6361 gcgccggcgg cccggacaac atcaccgccg tcgtggtccg gctcgtcgaa ggcccgcacg
     6421 gcgccgagga cgacgagacg acgctcgtgc aggccgtcga tcccgaggcg gacgtcgcca
     6481 ccggagagct gccgttcgtc gtcgccgctc ccgggggcgg ggagcccgcg ggcgacgccg
     6541 ccgacgcgct cgccgccgag gcggaggcgt tgcgtgcgga agaggcgggc agcacggtgg
     6601 ccgccgacgt ggccgccgcc gcggcccgcc gccgccgccg ctccgtgcgc cgcctgggca
     6661 tcgtcgcggc ggcgctcggc ctgctcaccg ccgcgctcgc cgccggcgcg ttcttcctca
     6721 gcacgatgtt cttcatcggc gtcgacgacg gcacgctgac cgtctacagc ggcgcgccgg
     6781 tcaagatcgg gcccgtcccg ctgcacgccg tctaccgccg cagccacgtg gcgtacgagt
     6841 cgctctcgcc ctcggcgcgc agcctcgtgg atgagcagca gctccgcgac cgccaggacg
     6901 cgctcgacct cggcgggctg ctggggatgt ggcgatgagc cggcgcaacc tcgagctcgt
     6961 ctttctcgtg ctcgccggcg ccgtgagcac catggcctac gtgtccgtct acgccgggcg
     7021 gttccgcgag atcaaccgca tctccatcat ctacgggctg gtgttcgtcg cgatcttcgt
     7081 gctgctgcac ctggtcgagc gcatcttcct gccgcaggcc gactcgttcc tgctcccgat
     7141 caccgcgctg ctcgcggcca tcggcctcac ggagatcttc cgcatcaagc cggcgctggc
     7201 gctcacccag gggcagtggc tgctggtcgg cgccggtctc ttcgtcctga cggtcgtgat
     7261 cgtgcgcgac cacgtgaagc tcgaccggta ccggtacctc atcggcgccg taggcctcgc
     7321 gctgctcgtg atcaccatcc tcgccggcac gaccatcaac ggcgccaagc tctggatcca
     7381 cgcgttcggg ttcagcatcc agccgtcgga gttcgccaag ctggcgatcg tgatcttcct
     7441 cgccggctac ctggacgaca agaaggtgat gctctcggtg ccgcagcgcc acgtgctcgg
     7501 cgtcccggtg ccggcgttca agtacttcgg accgctgctg gtgatgtggg tgctgtccct
     7561 cgccatgctg gtcttcatga aggacttcgg catggcgttg ctgttcatgt ccatcttcgt
     7621 ggggctgatg tacatggcca cggcgcgcgt cgtgtacgtg gtcgccggca tcgggctctt
     7681 cgccgccgcc ggcgccatcg cgctggccat cgtcccgcac gtgcaggacc gcttcaccgt
     7741 ttggctggac ccatggccgt acgccgccac gaccggctat cagttgctgc agtcgttgtt
     7801 caccatcgcc gacggcggcg tcgtcggcgc cggcttcggg cgcgggtacc tgctcttcgc
     7861 cgaccgccag cccgtggtgc ccgacctgca gaccgacttc atcttcacgg ccatcgccaa
     7921 cgagatgggc ctgctgggcg ccgccggggt cgtcctcgtg tacctgctct tcgtgtggcg
     7981 cggcttccgc atcgccgtct tcgcgccgga cgggttctcc aagctgctcg cagccggcct
     8041 ggcgacggcg ttcgccctgc agaccttcat catcatcggc ggcgtgacgc ggctcatccc
     8101 gctgaccggc atcacgctcc cgttcgtgag ctacggcggc agctcgatca ccgccaacct
     8161 gatgctcgtg gcgctcctgc tcatggtgtc caaccgctcc aacatcatcc gtggcggcac
     8221 cgagctcgag cggcgccgtc tggcggagag ggggtgacgc cgtgaaccgg gcgatgtcca
     8281 aggccttcgt catcaccgtg gtgttgttcg tggcgctcat cgccaacctc acctggatca
     8341 tggtcgtgcg cgcgcagtgg ttccaggacc ggccggagaa caagcgcagc atcgccaagg
     8401 agatgaagat caagcgcggc gacatcctcg ggtacgacgg gtcggtcatc gccggcacgg
     8461 agcgccgctc gggctactac caccgccgct atccgagcgg gacgctggcg ccgcagctgc
     8521 tgggctacga ctcggtgcgc tacgggcgga gcggcatcga ggcgcagctc aacgacgagc
     8581 tgacgggcca gtcgagcgat ctcggggtgc agaactgggt ggacaagctg ctggggcgcc
     8641 gacccgaggg cgcggacgtc aggctcaccc tcgtgccggc ggtccagaag gtcgcccagt
     8701 ccgcgctcaa ggggcagaag ggcgccatcg tcgtgctcga tccgacgacc ggcgcgctga
     8761 tcgcctcggc ctcggcgccc acctacgacc cggccaatct cgaggacagg tgggccaggc
     8821 tcaggaagga cgcctccgca ccgctgctca accggcccac ccagggcctg tacgtgcccg
     8881 gctcggcgtt caaggtcgtc accgcgtcgg gcgggctcga caccggccgg gtcacgcccg
     8941 acacggagtt cgtcgacacc ggcacctacg tggtgttcgg cggcaaggtg accaactacg
     9001 gcggcgccgt gtacggcccc aacgacttca ccacggcgct cacgctctcg atcaacacga
     9061 ccttcgccaa ggtcggcaac ctgctcgggc gcaagcgcct catcgccgcc gccgagcgct
     9121 acggcttcta ccagaagcct ccgctcgagc tgcccgccgg cgaggtcgtg gccagcggcc
     9181 gctacggcaa gaaggggctg ctcgatcccg acgcgttcat ggacccgctc gacgtcgcct
     9241 gggcggcctg cgggcaggag aggctgctgg ccacaccgct gcagatggcg ctcgtggccg
     9301 gcggtgtggc caacggcggc cgcgtgatga agccgtacta cctgcaggag gtcgtgacgc
     9361 ccgacggccg cgtcgtccgc acgacgcagc cggagcagtg gctcgcggcg accaaggcgc
     9421 tcacggccgg cgagctcaac ctgatgatgc agcgggtggt gaactcgggc accggcaccg
     9481 ctgcggcgct cgagggcatc caggtggccg gcaagaccgg gacggcggag gcgggcggcg
     9541 gctccaacct cgcctggttc atcggcttcg ccccggcgga cgatccgcag gtggccatcg
     9601 ccgtcgtcat cgaggacacg cagtcgacgg gcggcgaggc ggccgcgccg ctcgccgccg
     9661 cggtgatcaa ggcggcgctg gcgcagcccg ccctgccctg acgggcaggg accaggcgcc
     9721 cgtggtcgta agcttgacgg agaaggagag aggcaggcgg acgtgagtga cgtgttccag
     9781 ccagaacagg tgatcggcgg ccgctaccgg gtggtgcgca agctcggcgg cggcggcatg
     9841 gccgacgtgt atctgtgcga ggacctcacc ctcggccggc acgtcgccat caaggtgctg
     9901 ctccagcggt acctcaacga cgcgaccttc gtggagcgct tccgccgtga ggccaaggca
     9961 gcggcggggc tcaaccagca gaacctcgtc gccatctacg actggggcga ggtcgacggc
    10021 acgtactaca tcgccatgga gtacgtggag ggcgagaccc tcaaggacct catccgtcgt
    10081 cgcggcaggc tcagcggcaa cgaatcggtg gccgtggcga tgcagttgct ggccgcggtc
    10141 gagttcgccc accgcagcgg catcgtgcac cgggacatca agccgcagaa cgtgatgctc
    10201 gaccgcggcg gcacggtcaa ggtcatggac ttcggcatcg cccgcgccgg cgactccggg
    10261 atgacggagg ccggctccat cctcggcacc gcccagtacc tggcaccgga gcaggccatg
    10321 ggccacccgg tggacgagcg ctccgacctg tactccgtcg gcgtcgtcct gtacgagatg
    10381 ctcaccggca ccgtgccctt caagggcgac agcgccgtca cggtcgcgct caagcacgtc
    10441 aacgaggtgc cgcgagagcc gtcggagctg gtgccgggca tgccctacgc gctcaaccag
    10501 atcgtgctca aggcgatggc caaggacccg gccgacaggt accagtcggc ggccgagttc
    10561 gcgcgcgacc tgcgcgccgc cagggagggc ggccccgtcc aggcggccgc cttcgacgcc
    10621 ggcggcgagc gcacgcgcgt gatgaccggc gccgccggca tgacggcggc cgaggcgacc
    10681 tcggtgctcg accagccgct gccgccgcgc cgcaagaagt ccaggtggcc gctcatcctg
    10741 atcgtcctcc tgctggccat catcgccgcc gtcgccgtcg cgctgtggtg gacgatgagc
    10801 ggcaacaagg cagaggtccc cggcgtcgtc ggcctcagca gggccgccgc cgtcagggct
    10861 ctggagcagg acggcttcaa ggcggcggtc cagcaggagt tctccgagcg ggtggccgag
    10921 ggcttcgtca gccgccaggc gcccaccgcg gggaccaagc tgcgcgaggg cgccacggtc
    10981 gacatctggg tgagcaaggg cagccagaag gcggagcccc tgcccgactt ctccggctgg
    11041 acgccgtcca tggtggacga ctggctcgcc cggaacgatc tgcagggcga cccgcgcacg
    11101 ggcaggtcca acgccgtgag cgaagggcag gtgttccggc aggacccggc cccgggtacc
    11161 cagctcaacc gcggcgacac cgtctcctac tgggtcagca ggggcaagcc gcaggcgacc
    11221 gtgccggacc tgaccaacct cacgcaggcc gacgcggagg cggcgctggc cgaggccggg
    11281 ctcaggctcg gcagcgtcac gccggagacc agcgagacgg tgccggcggg cgcggtcgtc
    11341 aggcaggatc cgccggccgg cgagaaggtc gacaaggact ccgtggtgaa catcgtcgtc
    11401 tcctcggggc cacctacgcc gagtccctcc ccgacggtga gcccgtcggg agtggcggtg
    11461 cccaacgtct acggcatgca gtcctcgatc gcggagcagg agctcgtcgc gctcggcctc
    11521 gccgcggcgt tccgcgagaa gcccaacacc ggccagcagc ccggcaccgt ggtcagcgtg
    11581 aagccggacg ccggcacggt cgtgcccgcg gggtcgaccg tgacgctggt catcgcctcg
    11641 tagccgggga ggccggagcc ggaccccggg catcccgctc gcagacgaga aaggggcggg
    11701 ccgccgttcg gcggc
//
LOCUS       DASUMX010000043         9195 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_154971, whole genome shotgun sequence.
ACCESSION   DASUMX010000043 DASUMX010000000
VERSION     DASUMX010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 9195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9195
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154971"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            250..1938
                     /locus_tag="VFZ86_01930"
     CDS             250..1938
                     /locus_tag="VFZ86_01930"
                     /inference="COORDINATES: protein motif:HMM:NF037653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5641078.1"
                     /translation="MHTISDAGALTVRELLEHSGLGLTAATGEVGLGRVVKGIHFSDA
                     DDPVRYLTSESVLVATGRTWYQDVSAGLYQLDRLASIDTAALVVALGHFVDEIPAEMV
                     ARARELRLPVLLLPAGSLTRTVLSYVYHALASADLHRLRRTVAMQNDLLDLLIAEAGV
                     DELLAKVSLLIGMPMLLLDGTGTLVTSMGEVDPHRRAREVWEHWAELTDASALGIVEV
                     GDSRFFCRDVLLYGKLERLIVAAASQTSSSEFVDMALSFLQRLITLDLLKRRDEIVAT
                     RRLRRRLLRDLLTGSGALDELTALVAEHGVDLQKPWRVALCDFGGWPRGGRRADELED
                     ALVEAVDTFCGDRLIALLSRPKDTSLAVLLPDGYPSVGDGGARDLARGIKTFAAGEPY
                     RLQLAVGLSGAHRGPVAGPQALQEAIDALGAAQRGIDAQGLVLFEELSGRFRLLQGQS
                     GGALADIARRSVAPLLEYDARRHTHLLDTLRTLLDNHFAIQPTAEALFIHRNTLQKRM
                     RRIETLLGVDLADIDDLMELYLGLRALQLVGEAKVLERGQPLRLRPRPALTPPA"
     gene            complement(1922..2401)
                     /locus_tag="VFZ86_01935"
     CDS             complement(1922..2401)
                     /locus_tag="VFZ86_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00725 family protein"
                     /protein_id="HEX5641079.1"
                     /translation="MSAPRYVSVIGASRAAPELAAKAEELGELLAERGLVVVCGGRDG
                     VMAAVARGVKRAGGVSIGILPEADRQRAAPDLTYTVCSAMGYARNLSVVASGDVVVAL
                     GGAWGTLSEIALARNVGKPVILLDTWTIAPPGGGELDGVRRAATPAEAVELALTLEA"
     gene            complement(2398..2979)
                     /locus_tag="VFZ86_01940"
     CDS             complement(2398..2979)
                     /locus_tag="VFZ86_01940"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5641080.1"
                     /translation="MRDDITIEPLDAADVELVAPLWKQLMDHVASLPEGLVPVRPSDE
                     SWAMERAEMLRELAGEAFVLVARKDEAIVGYAFVCIEGPDPVWYTGDTHAVLASLCVD
                     ATERGDGIGGALLDAVDAELERRGVEDVEIGVDTGNEAAARLYESRGYRADFRIFYGS
                     PGAKPWACLRREAEDRAAGRGRFAPPGPTEGTA"
     gene            complement(3007..5325)
                     /locus_tag="VFZ86_01945"
     CDS             complement(3007..5325)
                     /locus_tag="VFZ86_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX5641081.1"
                     /translation="MDHDAGMTPKAARALQAFFTGGGHDQVVAVAHEAARRGRAADLP
                     ADLPAALREALSAAGVQSLHGHQAVARDLLAAGENVVVSTGTASGKSLCYQLAVLETF
                     VADPQARALFLFPTKALAQDQARKLVRFRVPGVVPAIYDGDTPQDQRPLLRRSATILL
                     TNPDMLHVGILPGHERWAEYLHQLRYVVLDEAHVYRGVFGSHVAQVVRRLRRLCGQYG
                     SDPRFVLTSATIGNPKEFAERLVGAPFSVVDEDQAPRPERTVVLWNPPLEDPASGVRR
                     SALAEASYVTAECVLAGSRVIAFAPTRKAAELVYGHVRRRLEDRRGRGAAERVQPYRA
                     GYTPEQRRDIERRLFSHELDAVVATQALELGIDVGGLDVSLVTGFPGTITSLRQRWGR
                     AGRSGHGYAVLVAGQDALDQYFMREPDRLLGRSVEEAIIDLHNPHISGPHLEAAAYER
                     PLTAGDEAYFGETGLLQAERLAQAGRLRRRKDGLVWSKPYSPASAVGLRTASNEQFAI
                     VEGRQGEVIGTVERERVFRFCHPGAVYLHLGRSYLVQHLDLETRTVLLDDFDGSYYTQ
                     AKTDKNVLIAGQADARELPGAVLFAGDLEVTEQVIAYQRRDMTDGHILDTQTLDLPEQ
                     TFTTQAFWLAVPQTVIDQALAQLGETDDLAAPGSLHAAEHALIALLPLYAMCDRWDIG
                     GLSTPWHWQTDTPSIFVYDGYPGGIGLSRRGYDAFESLAAAARALVAECPCESGCPSC
                     VQSPKCGNLNEPLSKAGAVSLLSAVLEAAPVR"
     gene            complement(5400..5927)
                     /locus_tag="VFZ86_01950"
     CDS             complement(5400..5927)
                     /locus_tag="VFZ86_01950"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEX5641082.1"
                     /translation="MKLHKSMIIVLIAIVCTLLLAAPALAATPLSSYEKQVAALVNKQ
                     RAKFGLPQLRIHSRLVEAARVHSADMGQRKYFQHDSPSGETWSSRIVRCGYTRKGYRY
                     WKAGENIYYGSGLYSSPLAVVRAWMKSPPHRAVILTRVFRDMGVGAVKTTDGFGGING
                     TVWLFTLDLGRRIAQ"
     gene            6201..6914
                     /locus_tag="VFZ86_01955"
     CDS             6201..6914
                     /locus_tag="VFZ86_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641083.1"
                     /translation="MVALVVFVTLITVAVWVAYAIPGQTPARVPAGAAEPTLGRTAPP
                     DEAVVVARVEDVDVLLPVSRAVTTAVAFHPVDNSDAVAFSPVGKRLGGGDLGQKLAGI
                     FAGDGELQYYLMDGDGGEASPSTAGLDVGAVPGSPVVSPVDGRVTAIKKYSVLGRYND
                     VEIDIRLTADPSLLLIVTHIAQPKVQIGDVVQRGTTALGVTRGFPAALQQGLSQYTSD
                     NGDHVQLVVVRVTPELSDL"
     gene            6977..7786
                     /locus_tag="VFZ86_01960"
     CDS             6977..7786
                     /locus_tag="VFZ86_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylase"
                     /protein_id="HEX5641084.1"
                     /translation="MPAATRAVIGGSGSHAAGFPGDLHPGAQVLDEGLVFETPWGESP
                     PFTLFELGGQRTLHVRMHGWRKGVSRGRASRQVFSVLHRAGVRRILSDAGVGSLNHLL
                     DPGDLVVVDDFVDMTTDRDRYGAVAGGHLLIMRDPVCATGREALAGAARRQAPERRLF
                     TRGTYVVTEGPRFESPAEVRHLQTLGDVVGQSFSPEVWLARDIGACYAGIYLIVNYAE
                     GVVREWEHEVLAQIFREDAGLMGRILLDALAALPPGDDCHCRELRKPTLLG"
     gene            complement(7835..8767)
                     /locus_tag="VFZ86_01965"
     CDS             complement(7835..8767)
                     /locus_tag="VFZ86_01965"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEX5641085.1"
                     /translation="MQAGAEPPSGAAGERHVPPPEVPGREVAAGPAWRPAAEPGPAAP
                     VPAAPPIAPSTDASGPAAPGVAPSPGGGPPLRAAAPGQPLYLPQASWRWPQALAGLAV
                     GAAPEALLSLASLLGDAGSSSRGAVSAGSAALLAVSALVLYGWQGLAAWLFSLRTAGR
                     ALALWGFRRPNRAILWTVPVGLVAVYAVSLAHDLVVHPEQQPIVNEFPRSGAGAALFV
                     LVAVVMAPLFEEIVFRGFLFRGFANSWGWVWGAIASAAVFGLAHLQLDVFVPLAALGF
                     ALAWAYKRTGSLWSCIAMHGIFNAIAVLAWALTG"
     gene            9086..>9195
                     /gene="rpmG"
                     /locus_tag="VFZ86_01970"
     CDS             9086..>9195
                     /gene="rpmG"
                     /locus_tag="VFZ86_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008538901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEX5641086.1"
                     /translation="MRQLVTLACQECKRRNYHTNKNKKNDPDRIELKKYCR"
BASE COUNT         1242 a         3307 c         3359 g         1287 t
ORIGIN      
        1 tgggcatcga ccggctgggc gccgtgacgg ctgtgggtgg ggcggaactt ccagagcctg
       61 ccgcggtcgt cgaaggtgag gctgaggtcg ttctcgatga gccagtcctg tcgcatggtc
      121 tctcctcttg acgctgccgg cggcgggcgc cggggtacat gtgtgtcgtt gtccgcgatg
      181 gtactctagc cgtgttcgca gcgtgatgac ggcacccgga cgcacactct tccgcgtgga
      241 ggtagtcact tgcacaccat cagcgacgcc ggcgccctca ccgttcgcga gcttctggag
      301 cactccggcc tcggcctcac ggcggccacc ggcgaggtgg ggctcgggcg ggtcgtcaag
      361 ggcatccact tctccgacgc cgatgacccg gtgcggtacc tgacgagcga gagcgtgctc
      421 gtcgccacgg gacgcacctg gtaccaggac gtcagcgccg gcctctacca gctcgaccga
      481 ctcgccagta tcgacaccgc ggcgctcgtg gtcgccctgg ggcacttcgt cgacgagatc
      541 cccgcggaga tggtcgctcg ggcgcgggag ctcaggctgc cggtactcct gttgccggca
      601 ggctcgctca cgcgcaccgt gctctcctac gtctaccacg cgctcgcgtc cgcggacctg
      661 caccgcctgc gccgcacggt ggccatgcag aacgacctcc tcgacctgct catcgcggag
      721 gcgggcgtgg acgagctgct cgccaaggtc tcgctgctga tcggcatgcc gatgctgctg
      781 ctcgacggca cgggcacgct ggtcacgagt atgggcgagg tcgacccgca ccggcgcgcg
      841 cgggaggtgt gggagcactg ggccgagctc accgacgcga gcgccctggg gatcgtcgag
      901 gtgggcgact cgcggttctt ctgccgcgac gtcctgctct acggcaagct ggagaggctc
      961 atcgtggcgg cggcgtcaca gacctcgagc tccgagttcg tggacatggc gctctccttc
     1021 ctgcagcggc tcatcacgct tgacctgctc aagcgccgcg acgagatcgt cgccacgcgg
     1081 cgcctccgcc gccgtctgct gcgcgacctc ctcaccgggt caggcgccct cgacgagctg
     1141 accgcgctgg tcgccgagca cggggtggac ctgcagaagc cttggcgagt ggccctctgc
     1201 gacttcggcg gctggccacg cggggggcgg cgcgccgacg agctcgaaga cgcgctggtg
     1261 gaggcggtgg acacgttctg cggggaccgc ctcatcgcgc tgctcagcag gcccaaggac
     1321 acatcgctcg ccgtgctgct tccggacggc tacccgtcgg tcggcgacgg cggggcgcgc
     1381 gatctggcgc gcggcatcaa gacgttcgcc gccggcgagc cgtaccggct gcagctcgcc
     1441 gtcggcctct ccggcgctca tcgtggtccc gtcgccggcc cccaggctct gcaggaggcg
     1501 atcgatgccc tgggcgccgc gcagcggggc atcgacgcgc agggcctcgt cctcttcgag
     1561 gagctgagcg gcaggttccg gctgctgcag ggtcagagcg ggggagcgct ggccgacatc
     1621 gcgaggcgca gcgtcgcgcc gctgctcgag tacgacgcca ggcggcacac ccacctgctg
     1681 gacacgctgc gcacgctgct ggacaaccac ttcgccatcc agcccaccgc cgaggcgctg
     1741 ttcatccatc gcaacaccct gcagaagcgc atgcggcgca tcgagacgct gctcggcgtc
     1801 gacctcgccg acatcgacga cctcatggag ctgtacctgg ggctgcgcgc gctgcagctc
     1861 gtgggagagg ccaaggtgct ggagcgcggc cagccgctcc ggctcagacc gcggccggcg
     1921 ctcacgcctc cagcgtgagg gcgagctcga cggcttcggc gggcgtggcc gcacgccgca
     1981 ctccgtcgag ctctccgcct ccgggcggcg cgatggtcca cgtgtccagc aggatcaccg
     2041 gcttgccgac gttgcgcgcc agcgcgatct cgctgagcgt gccccaggcg ccgccgagcg
     2101 cgacgaccac gtcgccgctg gcgacgacgc tgaggttgcg cgcatagccc atggcgctgc
     2161 agacggtgta cgtgaggtcg ggggcggcgc gctgacggtc ggcctccggg aggatgccga
     2221 tcgagacgcc gcccgcccgc ttcacgccgc gcgccaccgc cgccatcacg ccgtcgcgcc
     2281 cgccgcacac gacgacgagg ccgcgctccg cgaggagctc tccgagctcc tccgccttcg
     2341 cggccagctc cggcgcggcg cggctggcgc cgatgacgct gacgtacctc ggcgcgctca
     2401 tgccgtcccc tcggtggggc cgggcggcgc gaagcggccg cgcccggccg ctcggtcctc
     2461 ggcctcgcgg cgcagacagg cccacggctt cgctcccggc gagccgtaga agatgcggaa
     2521 gtcggcgcgg tagccgcggc tctcgtagag gcgcgcggcg gcctcgttgc ccgtgtccac
     2581 gccgatctcg acgtcctcga cgccgcgccg ctccagctcg gcgtcgactg cgtccaggag
     2641 cgcgccgccg atcccgtcgc cgcgctcggt cgcgtcgacg cagagcgacg cgagcaccgc
     2701 gtgcgtgtcg cccgtgtacc agacgggatc cggcccctcg atgcacacga acgcgtagcc
     2761 gacgatcgcc tcgtccttgc gggcgacgag cacgaacgcc tcgcccgcga gctcgcggag
     2821 catctccgct cgctccatcg cccaggactc gtccgagggg cgcaccggga cgaggccctc
     2881 cggcagggag gccacgtggt ccatgagctg cttccacagc ggggccacga gctcgacgtc
     2941 ggcggcgtcc agcggctcga tggtgatgtc gtcgcgcatg cgtcctccgg gcgggtgggg
     3001 tcgagcctat cgcaccggag cggcctcgag caccgcactc aacagcgaga cggcgccggc
     3061 cttgctcagc ggctcgttga ggttgccgca cttggggctc tgcacgcagc tcgggcagcc
     3121 gctctcgcag gggcactcgg cgacgagcgc ccgcgcggcg gcggcgaggc tctcgaaggc
     3181 gtcgtagccg cggcgcgaca ggccgatgcc gccgggatag ccgtcgtaga cgaagatgct
     3241 cggcgtatcg gtctgccagt gccacggggt gctgaggccg ccgatgtccc agcggtcgca
     3301 catcgcgtac agcggcagga gggcgatgag cgcgtgctcg gcggcgtgga gtgaaccggg
     3361 cgccgcgagg tcgtcggtct cgccgagctg agccagcgcc tggtcgatca ccgtctgggg
     3421 gaccgccagc cagaaggcct gcgtcgtgaa ggtctgttcg gggaggtcca gcgtctgcgt
     3481 gtcgaggatg tgcccgtcgg tcatgtcgcg gcgctggtag gcgatcacct gctcggtgac
     3541 ctcgaggtcg ccggcgaaga ggacggcgcc gggcagctcg cgcgcgtccg cctgcccggc
     3601 gatcagcacg ttcttgtccg tcttggcctg cgtgtagtag ctgccgtcga agtcgtccag
     3661 caggacggtc cgcgtctcga ggtcgaggtg ctgcacgagg tagctgcgcc cgaggtggag
     3721 gtagacggcg ccggggtggc agaagcggaa gacgcgctcg cgctcgacgg tgccgatgac
     3781 ctccccctgt cgcccctcga cgatggcgaa ctgctcgttg ctcgcggtcc gcaggccgac
     3841 ggcggaggcg ggcgagtagg gcttggacca gacgaggcca tccttgcgcc gccgcaggcg
     3901 gccggcctgc gccagccgct cggcctgcag caggccggtc tcgccgaagt aggcctcgtc
     3961 gccggcggtc agcggccgct cgtaggccgc ggcctcgagg tgcggcccgc tgatgtgcgg
     4021 gttgtggagg tcgatgatcg cctcctcgac gctgcgcccc agcaggcggt cgggctcgcg
     4081 catgaagtac tggtcgagcg cgtcctggcc ggccaccagc acggcgtagc cgtgcccgga
     4141 ccggccggcc cttccccagc gctggcgcag gctggtgatc gtgccgggaa agccggtgac
     4201 gagcgagacg tcgaggccgc cgacgtcgat ccccagctcg agcgcctgcg tcgccacgac
     4261 ggcgtcgagc tcgtgcgaga agaggcgccg ctcgatgtcg cgccgctgct ccggcgtgta
     4321 gccggcgcgg tacggctgca cgcgctcagc ggcgccgcgg ccgcgccggt cctcgaggcg
     4381 gcggcggacg tgcccgtaca ccagctccgc cgccttgcgc gtcggcgcga aggcgatcac
     4441 gcgcgacccg gcgagcacgc actccgcggt gacgtagctc gcctccgcga gcgcgctgcg
     4501 gcgcacgccg gaggcggggt cctccagcgg cgggttccag agcacgaccg tgcgctcggg
     4561 gcgcggcgcc tggtcctcgt cgacgacgga gaagggcgcc ccgacgaggc gctccgcgaa
     4621 ctccttcggg ttgccgatcg tcgccgaggt gagcacgaag cgcgggtcag aaccgtactg
     4681 gccgcagagc cgccgcaagc ggcgcacgac ctgggcgacg tggctgccga agacgccgcg
     4741 gtagacatgc gcctcatcga ggaccacgta ccgcaactgg tgcaggtact cggcccagcg
     4801 ctcgtgcccg ggcaggatgc cgacgtgcag catgtcgggg ttggtgagca ggatcgtcgc
     4861 gctgcgtcgc agcagcggac gctggtcctg gggcgtgtcc ccgtcgtaga tggccggcac
     4921 cacgccgggc acacggaacc ggacgagctt gcgcgcctgg tcctgggcga gcgccttggt
     4981 ggggaagagg aagagggccc gcgcctgggg gtcggcgacg aacgtctcga gcacggcgag
     5041 ctggtagcag aggctcttgc cgctcgcggt cccggtgctc acgaccacgt tctcgccggc
     5101 cgccaggagg tcccgggcga cggcctggtg accgtggagc gactggacgc cggccgcgga
     5161 gagcgcctcg cgcagcgcgg ccggcaggtc cgcggggagg tcggcggcgc ggccgcgccg
     5221 cgccgcctcg tgcgccacgg cgacgacctg gtcgtgcccg ccgcccgtga agaacgcctg
     5281 cagcgcacgg gcggccttgg gggtcatgcc ggcgtcgtgg tccacgccgc catggtactc
     5341 cacgcgaaga ggggcgctcg cgcgcccctc cgctcgactc tcctggtctg ccgccggggc
     5401 tactgcgcga tccggcgccc caggtcgagc gtgaagagcc acacggtgcc gttgatgccg
     5461 ccgaacccgt ccgtggtctt gaccgcgccc acgcccatgt cgcggaagac cctggtgagg
     5521 atgaccgccc ggtgcggcgg ggacttcatc caggccctga cgacggcgag cgggctcgag
     5581 tacaggccgc tgccgtagta gatgttctcg ccggccttcc agtagcggta gcccttgcgc
     5641 gtgtagccgc agcggacgat gcgcgacgac cacgtctcgc cggacggcga gtcgtgctgg
     5701 aagtacttgc gctggcccat gtccgccgag tggacgcgcg cggcctcgac cagcctgctg
     5761 tggatccgga gctgtgggag gccgaacttg gcgcgctgct tgttgaccag cgccgcgacc
     5821 tgcttctcgt agctgctgag tggagtcgca gcgagcgccg gcgccgcgag cagcagcgtg
     5881 cacacgatcg cgatgaggac gatgatcatg ctcttgtgca gcttcatttg ctcacccccg
     5941 aggccggccc ggccatcacg aacggatctg ggccgttgcc cgcgacccct gttcgactct
     6001 ggtatcggtg gagtccgcca ggagttgagt catcggccgg aagaaagcat cgtctgggtt
     6061 gacgacaccg gccgctgcac ggcggcggct tgcggccgtg cggggagcgt tgtagcttca
     6121 ggagcgacat gcacacaagc ccggccgatc agcggcggcg acatggtgct cgccgccgcc
     6181 accagattcg ccgccggcgt gtggtcgcgc tggtcgtctt cgtgaccctc atcaccgttg
     6241 ccgtgtgggt cgcatacgcc atccccggcc agacgccggc gcgcgtaccc gcgggcgccg
     6301 ccgagcccac cctcggacgc accgccccgc ccgacgaggc ggtggtcgtc gcccgggtcg
     6361 aggacgtcga cgtgctgctc ccggtctccc gcgcggtcac caccgcggtg gccttccatc
     6421 ctgtggacaa cagcgacgcc gtcgccttct ctcccgtcgg caagcggctc ggcggcggcg
     6481 acctcggtca gaagctcgcc ggcatcttcg cgggggacgg cgagctgcag tactacctca
     6541 tggacggcga cggcggagag gcgtcgccct cgacggccgg cctcgacgtg ggcgcggtcc
     6601 ccgggtcgcc ggtcgtctcg ccggtggacg gcagggtgac ggcgatcaag aagtacagcg
     6661 tcctgggccg ctacaacgac gtcgagatcg acatccggct gaccgcggac ccgtccctgc
     6721 tgctcatcgt cacgcacatc gcccagccca aggtgcagat cggcgacgtg gtacagcgag
     6781 gcacgaccgc tctgggcgtc acgcgcggct tccccgcggc gctccagcag gggctcagcc
     6841 agtacacctc ggacaacggc gatcacgtgc agttggtcgt ggtgcgggtg acgccggagc
     6901 tgagcgacct gtgaatggcc gcgagcaagc tgtcgagccg ggcgccgaag gggcgccgct
     6961 ccgcgccgcc tccgctgtgc ccgccgcgac gcgcgcagtc atcggcgggt cgggctccca
     7021 cgccgccggg tttcccggcg acctccaccc cggggcgcag gtgctggacg aaggtctcgt
     7081 cttcgagacg ccgtggggcg agagcccgcc gttcacgctc ttcgagctcg gcgggcagcg
     7141 gacgctgcac gtcaggatgc acggctggcg caagggcgtc tcgcgcggcc gcgcctccag
     7201 gcaggtcttc agcgtgctgc accgcgccgg cgtcaggcgg atcctctcgg acgccggcgt
     7261 cggcagcctc aaccacctgc tggaccccgg cgacctggtg gtggtcgacg acttcgtcga
     7321 catgacgacc gaccgcgacc gctacggcgc ggtcgccggc ggccacctgc tcatcatgcg
     7381 cgaccccgtg tgcgcgaccg ggcgcgaggc gctggccggc gccgcgcggc gtcaggcgcc
     7441 tgagcgccgg ctgttcacgc gcggcacgta tgtggtcacc gagggtccgc gcttcgagtc
     7501 gccggccgag gtccgccatc tccagacgct cggcgacgtg gtgggccaga gcttcagccc
     7561 ggaggtctgg ctggcccgcg acatcggcgc ctgctacgcg ggcatctacc tcatcgtgaa
     7621 ctacgcggag ggcgtcgtgc gcgagtggga gcacgaggtg ctcgcgcaga tcttccgcga
     7681 ggacgccggc ctcatgggcc gcatcctgct ggacgcgctc gcggccctgc cgcccggcga
     7741 cgactgccat tgccgggagc tgcgcaagcc cacgcttctc ggctgacggc ggcggcgcgg
     7801 cgatccggtc gttctgcgcc gccggcggcc gccctcagcc ggtcagcgcc caggcgagca
     7861 ccgcgatcgc gttgaagatc ccgtgcatcg cgatgcacga ccacagcgac cccgtgcgct
     7921 tgtaggccca cgcgagggcg aatcccagcg ccgccagcgg cacgaagacg tcgagctgca
     7981 ggtgcgccag cccgaagacc gcagcagagg cgatggcgcc ccacacccat ccccaggagt
     8041 tcgcgaagcc gcggaagagg aacccgcgga agacgatctc ctcgaacagg ggggccatga
     8101 ccacggcgac gaggacgaac aacgccgctc ctgcgccact gcgcgggaac tcgttcacga
     8161 tcggttgctg ctccggatgc acgacgaggt cgtgcgcgag gctcacggca tagacggcga
     8221 cgagacccac ggggaccgtc cagaggatcg cgcgattggg acgccggaaa ccccagagcg
     8281 ccagcgcgcg gccggcggtc cggagcgaga agagccaggc cgcaaggccc tgccagccgt
     8341 agagcacgag ggcgctcacg gcgagcagcg ccgcgctgcc ggcgctcacc gctcctcgcg
     8401 agctcgagcc ggcgtctccg agcagcgacg ccagcgagag cagcgcctcc ggggcggcgc
     8461 cgaccgcgag gccggccagc gcctgcggcc agcgccacga cgcctgcggc aggtacaggg
     8521 gctggccggg agcggcggcg cggagcggcg gcccgccgcc gggagaaggc gcgacgcccg
     8581 gcgccgcggg tccggacgcg tccgtgctgg gcgcgatggg gggtgcagca ggtacgggcg
     8641 ccgcggggcc gggctcggcg gcgggccgcc atgccgggcc ggcagcgacc tcgcgccccg
     8701 gcacctccgg cggcggcacg tgccgctcgc cggcggcgcc ggacggcggt tccgcgccgg
     8761 cctgcacccg gcgttcgtct ccctcggact gtgcgtgtcc gtcgatcgcg tctcctcccg
     8821 tttcgctctc accctatcgg gaagcactcg agaatgggac tcccgacgcg tccccggcgt
     8881 acgcgctgag ccgcgtgcct ggcctgacgc tcatcgtgac gcccgacggt gacgccgttt
     8941 tgcccggctc cctccggcct gctatactcc gccgccgtgg ccgtcgcggg ccttgcagcc
     9001 gcgtgcgggg gccggagggc cgcgcgttga ccctcccgct gagcgcttgg tatccttacg
     9061 tgcttctgct tgaaaggaag acgacgtgcg tcaactcgtc acgctcgctt gccaggaatg
     9121 caagcgtcgc aactatcaca cgaacaagaa caagaagaac gacccggacc gcatcgagct
     9181 caagaagtac tgccg
//
LOCUS       DASUMX010000044         3047 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_730911, whole genome shotgun sequence.
ACCESSION   DASUMX010000044 DASUMX010000000
VERSION     DASUMX010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3047
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_730911"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1024
                     /gene="arsB"
                     /locus_tag="VFZ86_01975"
     CDS             <1..1024
                     /gene="arsB"
                     /locus_tag="VFZ86_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013645434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HEX5641087.1"
                     /translation="FIDRWSVGTTNVPIAIGLIVMMYPPLARVRYEELGDVFRDWKIL
                     GLSLVQNWVIGPFVMFFLAIIFLHDYPEYMVGLIMMGLARCIAMVIVWNQLAKGDTEY
                     AAGLVAFNSVFQVLFYSVFAWVFVTKLPPLFGLEGVAVDITIGEIARSVFIYLGIPFI
                     AGMVSRYIGLKTKGRDWYEHKFIPRISPLTLIALLFTIFVMFSLKGDLIVELPLDVLL
                     IAIPLSLYFVIMFFVSFFMGRKIGADYSKTATLSFTAASNNFELAIAVSIAVFGLNSG
                     QAFASVIGPLVEVPVLIGLVSVALWLGRRYWGMTDEPLPAAIEAGCDEVGCDPLDGHS
                     PTREAT"
     gene            1021..1395
                     /locus_tag="VFZ86_01980"
     CDS             1021..1395
                     /locus_tag="VFZ86_01980"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX5641088.1"
                     /translation="MTTPNEHVTIEELTPFFKAFCNPTRAQIVEFLLGGERCVCEMTG
                     PLDVSQPLVSHHLALLREAGLLSTRDEGARTYYSINRERFDADLAGFLAFVDRARNAP
                     RPVDAATSACATKQKRGVRAAS"
     gene            1419..1673
                     /locus_tag="VFZ86_01985"
     CDS             1419..1673
                     /locus_tag="VFZ86_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006715230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX5641089.1"
                     /translation="MKLEILGSGCANCQKLMAVTEAAVRDLGIDDAELVKVEDFPRIM
                     AYGVMSTPALVIDGQVVLSGKVPSQAEVSSLITTALAAQG"
     gene            1697..2107
                     /locus_tag="VFZ86_01990"
     CDS             1697..2107
                     /locus_tag="VFZ86_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX5641090.1"
                     /translation="MAATLAGAVAVALLVVACGGAAGDDASSTLPAATSSSGAAAKVT
                     FLELGADKCVPCKEMRPVMEGIEQAFGDQVEVIFYDVWDDPAPANEYGIQMIPTQIFL
                     DENGEEFHRHTGFYPQADIEALLVERGLEKIATP"
     gene            2114..2809
                     /locus_tag="VFZ86_01995"
     CDS             2114..2809
                     /locus_tag="VFZ86_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020676718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEX5641091.1"
                     /translation="MLEQILTSLSNGLGQSLGWALLAAFAWGVASIVFSPCHLASVPL
                     IVGFISTREDASTGRAFRLSLVFALGVLASIALIGVITWSLGRMVGDLGTIGNLAVAA
                     VFFVFGLYLMDLLPLRWAKVLPASTKRHGMPAALSLGLVFGIGLGPCSFAFLAPLLVM
                     VFAEASTGYVLAAGLLAAFALGHCGVIVLAGTAAQKAQTVVSWGDRSGVARWVRRGCG
                     ALVLLAGVYLLVG"
BASE COUNT          433 a         1084 c          984 g          546 t
ORIGIN      
        1 cttcatcgac cgctggtcgg tcggcaccac caacgtcccc atcgccatcg gtctcatcgt
       61 gatgatgtat ccgcccctcg ccagggtccg ctacgaggag ctcggcgacg tgtttcgcga
      121 ctggaagatc ctcggcctct ccctcgtcca gaactgggtg atcggcccgt tcgtgatgtt
      181 cttcctcgcc atcatcttcc tgcacgacta ccccgagtac atggtcggcc tcatcatgat
      241 gggcctcgcg cgctgcatcg ccatggtcat cgtctggaac cagctcgcca agggcgacac
      301 cgagtacgcc gccggcctcg tggccttcaa cagcgtcttc caggtgctct tctactcggt
      361 gttcgcctgg gtcttcgtga ccaagctgcc gccgctcttc ggcctcgagg gcgtcgccgt
      421 cgacatcacc atcggcgaga tcgccaggag cgtgttcatc tacctgggca ttccgttcat
      481 cgcggggatg gtcagccgct acatcggctt gaagaccaag ggccgcgatt ggtacgagca
      541 caaattcatc ccgaggatca gcccgctcac gctcatcgcc ctgctgttca cgatcttcgt
      601 catgttcagc ctcaagggcg acctgatcgt cgaactacct ctggacgtgc tcctgatcgc
      661 gatcccgctg tccctgtact tcgtgatcat gttcttcgtg agcttcttca tgggacgcaa
      721 gatcggcgcc gactactcca agaccgcgac gctgagcttc accgcggcga gcaacaactt
      781 cgaacttgcc atcgccgtga gcatcgccgt cttcggcctc aactccggcc aggcgttcgc
      841 cagcgtcatc ggcccgctcg tcgaggtgcc ggtgctcatc ggcctcgtga gcgtggcgct
      901 gtggctcggg cgcaggtact ggggcatgac cgacgagcct ctgccggccg cgatcgaggc
      961 cggctgcgac gaggtcggct gcgaccccct ggatggacac tcaccgacac gggaggcgac
     1021 atgacgacgc cgaacgagca cgtcacgatc gaggagctca ccccgttctt caaggcgttc
     1081 tgcaatccca cgcgcgccca gatcgtcgag ttcctgctcg gcggcgagcg ttgcgtctgc
     1141 gagatgacgg gaccgctcga cgtcagccag ccgctggtct cgcatcacct cgcgctgctg
     1201 cgtgaggccg gcctgctctc cacgagagac gagggggccc ggacctacta ctccatcaac
     1261 cgggagcggt tcgacgccga cctggccggc ttcctcgcct tcgtcgacag ggcacgcaac
     1321 gccccgcgcc ccgtcgacgc ggcaacgtcg gcgtgcgcca cgaaacagaa gcgcggcgtg
     1381 cgcgccgcct cctagtaccg acagcgaagg agagaccctt gaaactcgag atccttggtt
     1441 ccggctgcgc caactgccag aagctgatgg cggtcaccga agccgccgtg cgcgacctcg
     1501 gcatcgacga cgccgagctc gtcaaggtcg aggacttccc caggatcatg gcctacgggg
     1561 tcatgtcgac gcccgccctc gtcatcgacg gccaggtcgt gctgagcggc aaggtgccgt
     1621 cgcaggccga ggtcagctcc ctgatcacca cggcgctggc cgcccagggg tgatcgcccc
     1681 tttgcgaccg tggcggatgg cggcgacgct ggccggcgcg gtcgccgtcg ccctgctcgt
     1741 cgtcgcgtgc ggtggggccg ccggcgatga cgcgtcatcc accctgcccg ctgccacgag
     1801 cagctccggc gccgcggcca aggtcacctt cctggagctc ggcgccgaca agtgcgtgcc
     1861 ctgcaaggag atgcgccctg tcatggaggg catcgagcag gccttcggcg accaggtcga
     1921 ggtgatcttc tacgacgtct gggacgatcc ggcgccggcc aacgaatacg gcatccagat
     1981 gatccccacg cagatcttcc tcgacgagaa cggtgaggag ttccaccggc acaccggctt
     2041 ctatccgcag gcggacatcg aggcgctgct cgtcgagcgc gggctcgaga agatcgcgac
     2101 gccgtagccc gcagtgctcg agcagatcct caccagcctg agcaacggcc tcggccagtc
     2161 gctcggctgg gcgctgctgg cggcgttcgc gtggggcgtc gcgagcatcg tcttcagccc
     2221 ctgccacctg gccagcgtgc cgctgatcgt cggcttcatc tccacccggg aggacgcctc
     2281 gaccgggcgc gcgtttcggc tctctctcgt cttcgcgctc ggcgtcctcg cgtccatcgc
     2341 cctgatcggc gtcatcacct ggtcgctcgg gaggatggtc ggcgacctcg gcacgatcgg
     2401 caacttggcc gtggccgcgg tcttcttcgt cttcggcctg tacctcatgg acctgctgcc
     2461 gctgcggtgg gcgaaggtgc tccccgcgtc gacgaagcgt cacggtatgc ccgcggcgct
     2521 gagcctcggc ctggtcttcg gcatcggcct cggcccctgc tccttcgcct ttctcgcccc
     2581 tctgctcgtg atggtgttcg ccgaggcgag cacgggctac gtgctcgccg ccggcctgct
     2641 cgctgccttc gccctggggc actgcggcgt catcgtcctc gcagggacgg ccgcgcagaa
     2701 agcccagaca gtcgtctcgt ggggcgatcg ctcgggcgtc gcgcgctggg tgcggcgcgg
     2761 ctgtggagca ctggtcctgc tggccggcgt ctacctgctc gtcggatagc ggaggccggt
     2821 ccatggctcc gatcgtggtt gccgctcaga gtgcgccgcc gctaggctgg tggccatgac
     2881 ggagaagacc tgttgcggcg ccggcgcgcc gccttcccca ccggcgagac gcgccggatg
     2941 ctgcgagccg gcggagccgg cgtgctgcgc ggcgccggcg ccagaaccgc ctcagagctc
     3001 gtgctgcggg gcgccggaga gcgcgccggc ggactatgcc tacggcc
//
LOCUS       DASUMX010000045         3851 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_587355, whole genome shotgun sequence.
ACCESSION   DASUMX010000045 DASUMX010000000
VERSION     DASUMX010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3851
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587355"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..775
                     /locus_tag="VFZ86_02000"
     CDS             <1..775
                     /locus_tag="VFZ86_02000"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5641092.1"
                     /translation="TYKWTFSNAQGAEDNADALVKIFAQQGWKTVVGAGDVLTIQQET
                     LDYCAEMAPDQGITFTKLRDQWPLDQTDFSGLASKVVAACDEANADALALMITAPQAD
                     AMMKALKGAGLDIPVIGGPPLAQAIPLFAAGTENVDGMYLISTAVTAGPQLPDDYPAK
                     GMVVGFYEAVMAETNAPPDNFGAWAFDAVMLMNEAVTKAGSIDKEAVRSAFESITGFY
                     GVSGQYNYSADDHIGIHGGMWLFQIENGEYKLIGDAAIN"
     gene            995..1249
                     /locus_tag="VFZ86_02005"
     CDS             995..1249
                     /locus_tag="VFZ86_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005034076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HEX5641093.1"
                     /translation="MNNVVVDFDACTGCKTCYLACFADVIRWDAEAKRPIVAYPEDCV
                     RCNYCEISCKDEAIKVVADWDMDFPPVIERGFPYTVGGTR"
     gene            1246..2952
                     /locus_tag="VFZ86_02010"
     CDS             1246..2952
                     /locus_tag="VFZ86_02010"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5641094.1"
                     /translation="MTTLEQLGRVETADILVIGGGVGGLAAAAAAKEKNPDADVLVVE
                     KATTGWAGQANKGAGIWWYLAPEDDVDAFVKFHVEHIGHYIEDQDLLADFGRESLGIL
                     ERMDGWTGRLQRRDGEIIHKRFKPFLPWSLTGMDVDFMRPLRRTARKLGVRFIDKTSL
                     TDLLTDGGRVAGAVGFSLLDGSPIVVKAPAAIIATGGQSYRIMGMWSCQRGDGLGMAW
                     RAGAQMRNAEWGPFAQLAGKRGKEPLIGAEDALYNAWHEKLSPTFRGDQEADVCGMTG
                     VVWYRQMKAGNGPLLTFHPENWMLANTTEDVEGGTVWDRPHAVKFWQTLIGRVRSADA
                     PGAFLEVFPAVLGELSPIRVDHQMATTVPGLYACGNACYNGSGLPGAVPASPGRMRGA
                     GLYGATFMGIRAGEAAASAAASAEVDVAQAEALVRDALAPLERGAGVPAMELVRGIQN
                     AVNPAGYSIYKSEERMTEALGMVLDVKERLPQVTAQDPHYLVAAHDARNMALSAELFY
                     RTALTRQESRGWFIREDYPERNDAEWLKWVCAEPKGDGIRIWTEDVPVADYQYQPASE
                     RS"
     gene            2955..3800
                     /locus_tag="VFZ86_02015"
     CDS             2955..3800
                     /locus_tag="VFZ86_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005035957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEX5641095.1"
                     /translation="MAATMERQPAPLTPEEQAKPYAKFYTMEFPGPDPETVALMSPER
                     PIPQDQMLPAEEMNRLLDPGNFETEIGWGMMDNGAGYIAMRHEMPGVTVDMVDWWFAW
                     HPIEGLRYRIWYPPYHLGVHVDDWAQPRLLDPGVPVRDKCEKVVHHVTEDTGMGTEHI
                     DIHFLTPGQMGFDMSRWHKPNVGTFVGGFGWSVNEHQPPEIPAAPAIMVHFIREMDRG
                     VEWRTRFWMGYTILNGRPVPLLPPGIRVPEPVVFGLANHNIHEYTRLKSLLPLVYEEF
                     GGTFA"
BASE COUNT          645 a         1333 c         1315 g          558 t
ORIGIN      
        1 tacgtacaag tggacgttca gcaacgcgca gggcgctgag gacaacgccg acgcgctggt
       61 caagatcttc gcgcagcagg gctggaagac ggtcgtcggc gccggtgacg tcctgacgat
      121 ccagcaggag acgctcgact attgcgccga gatggcgccg gaccagggca tcaccttcac
      181 caagctcagg gaccagtggc cgctcgacca gaccgacttc agcggcctcg cctccaaggt
      241 ggtggccgcg tgtgacgagg ccaacgcgga cgcgctcgcc ctcatgatca ccgctccgca
      301 ggcagacgcg atgatgaagg ccctcaaggg cgccgggctc gacatcccgg tcatcggcgg
      361 cccgccgctc gcgcaggcca tcccgctgtt cgcggccggg accgagaacg tcgacggcat
      421 gtacctcatc tccaccgccg tcaccgccgg tccgcagctg ccggacgact atcccgccaa
      481 gggcatggtc gtcgggttct acgaggcggt catggcggag accaacgccc caccggacaa
      541 cttcggtgcc tgggcgttcg acgccgtcat gctgatgaac gaggccgtga ccaaggccgg
      601 cagcatcgac aaggaggccg tccgctccgc gttcgagtcg atcaccggct tctacggcgt
      661 ctccggtcag tacaactact ccgccgacga ccacatcggg atccacggcg gcatgtggct
      721 gttccagatc gagaacggtg aatacaagct catcggggac gcggccatca actgacgcgg
      781 ccgcgtcttc tcggctccgg gagggcaaac cacgccctcc cggagccttc tccgtctctg
      841 ctctcgcacc agccattccg tagcacgcgt tgacagccga ggcggctcac tctagcattc
      901 gtagtagtta ttctcagtcc tatagctgtt gaacaccgtc agctcgcggg ggccctgcca
      961 aggagccccc agggcgaggg aggtcgggcc agggatgaac aacgtcgtcg tcgacttcga
     1021 cgcctgcacc gggtgcaaga cctgctacct ggcgtgcttc gcggacgtga tccgctggga
     1081 cgcggaggcc aagcgtccca tcgtcgcgta tccggaggac tgcgtgcgct gcaactactg
     1141 cgagatcagc tgcaaggacg aggccatcaa ggtcgtcgcc gactgggaca tggacttccc
     1201 gccggtgatc gagcgcggct tcccgtacac ggtgggaggc acgcgatgac caccctcgag
     1261 cagctcggcc gcgtcgagac cgccgacatc ctggtgatcg gcggcggcgt cggcggcctc
     1321 gccgccgctg cggcggccaa ggagaagaac ccggacgccg acgtcctggt cgtcgagaag
     1381 gcgacgaccg gctgggccgg ccaggccaac aagggggccg gcatctggtg gtacctcgcg
     1441 cccgaggacg acgtggacgc cttcgtcaag ttccacgtcg agcacatcgg ccactacatc
     1501 gaggaccagg acctgctcgc agatttcggc cgcgagagcc tggggatcct ggagcgcatg
     1561 gacggctgga ccggccggct ccagcgccgc gacggcgaga tcatccacaa gcgcttcaag
     1621 ccgttcctgc cctggagtct caccggcatg gatgtggact tcatgcgccc gctcaggcgc
     1681 acggcgcgca agctgggcgt gcgcttcatc gacaagacgt cactcacgga ccttctcacc
     1741 gacggcggcc gcgtcgccgg cgccgtcggt ttcagccttc tcgacggctc accgatcgtc
     1801 gtcaaggcgc cggcggcgat catcgccacg ggcggccaga gctaccgcat catggggatg
     1861 tggagctgcc agcgcggcga cggcctgggc atggcctggc gcgcgggcgc gcagatgcgc
     1921 aacgccgagt gggggccgtt cgcccagctc gccggcaagc gcggcaagga gccgctcatc
     1981 ggcgccgagg acgcgctgta caacgcctgg cacgagaagc tctcccccac gttccgtggt
     2041 gatcaggagg ccgacgtctg cgggatgacc ggcgtggtct ggtaccggca gatgaaggcg
     2101 gggaacgggc cgctgctgac gttccacccg gagaactgga tgctcgccaa caccacggag
     2161 gacgtggagg gcggcaccgt gtgggaccgc cctcacgcgg tgaagttctg gcagacgctg
     2221 atcggccggg tgcgctccgc cgacgcgccg ggtgcgttcc tcgaggtctt cccggccgtg
     2281 ctgggcgagc tctcgcccat ccgcgtcgac caccagatgg cgaccacggt gcccggcctc
     2341 tacgcatgcg gcaacgcctg ctacaacggc tccggcctgc cgggcgccgt gccggcctcg
     2401 cccgggcgca tgcgcggggc aggtctctac ggcgccacct tcatgggcat ccgcgccggc
     2461 gaggcggcgg cgtccgccgc cgcgagcgcc gaggtcgacg tcgcgcaggc cgaggcgctc
     2521 gtgcgcgacg ccctcgcgcc gctcgagcgc ggcgccggcg tgccggcgat ggagctggtc
     2581 cgtggcatcc agaacgccgt gaatccggcg gggtactcca tctacaagag cgaagagcgg
     2641 atgaccgagg cgctcggcat ggtgctcgac gtcaaggagc gcctgccgca ggtgacggcg
     2701 caggatccgc actacctcgt ggccgcccac gacgcgcgca acatggcgct gagcgccgag
     2761 ctcttctacc gcacggcgct cacgcgccag gagtcgcgcg gctggttcat ccgcgaggac
     2821 tacccggagc ggaacgacgc cgagtggctc aagtgggtct gcgccgagcc gaaaggggac
     2881 ggaatccgca tctggaccga ggatgtcccg gtcgccgact accagtacca gccggcctct
     2941 gagaggagct gaccatggcc gccacgatgg agcgccagcc tgccccgctg actcccgagg
     3001 agcaggccaa gccttacgcc aagttctaca cgatggagtt ccccggtccc gacccggaga
     3061 ccgtcgccct gatgtccccg gaacgcccga ttccccagga ccagatgctg ccggcggagg
     3121 agatgaaccg gctgctcgac cccggcaact tcgagaccga gatcggctgg ggcatgatgg
     3181 acaacggcgc cggctacatc gccatgcgcc acgagatgcc cggcgtgacg gtggacatgg
     3241 tcgactggtg gttcgcctgg caccccatcg agggcctgcg ctaccgcatc tggtacccgc
     3301 cgtaccacct cggcgtgcac gtcgacgatt gggcgcagcc gcgcttgctg gatcccggcg
     3361 tgcccgtgcg cgacaagtgc gagaaggtcg tccaccacgt cacggaagac acagggatgg
     3421 gcaccgagca catcgacatc cacttcctca cgccggggca gatgggcttc gacatgagcc
     3481 gctggcacaa gcccaacgtc ggcaccttcg tgggcggttt cgggtggagc gtcaacgagc
     3541 accagcctcc ggagatcccg gccgcgccgg cgatcatggt ccacttcatc cgcgagatgg
     3601 accgcggcgt cgagtggcgc acgcggttct ggatggggta cacgatcctg aacggccggc
     3661 cggtgccgtt gctgccgccg ggcatccgcg tgccggagcc cgtggtgttc gggctcgcga
     3721 accacaacat ccacgagtac acgcggctca agtcgctgtt gccgctcgtg tacgaggaat
     3781 tcggcggcac gttcgcctga cggccgacgg agaaacgcgt gccggcgtgg gtccgcaggg
     3841 cccccgccgg c
//
LOCUS       DASUMX010000046         4617 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_874840, whole genome shotgun sequence.
ACCESSION   DASUMX010000046 DASUMX010000000
VERSION     DASUMX010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4617
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_874840"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..279)
                     /locus_tag="VFZ86_02020"
     CDS             complement(64..279)
                     /locus_tag="VFZ86_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009279561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641096.1"
                     /translation="MAARKQYLLRVDPALWAEVERWAADELRSVNAQVEYILRDAVRR
                     RRGGRAAGDAAASGGRGGGPSGRTRTS"
     gene            complement(309..1346)
                     /locus_tag="VFZ86_02025"
     CDS             complement(309..1346)
                     /locus_tag="VFZ86_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009279562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX5641097.1"
                     /translation="MAADQKTPTKEREARVMSGWPMLVLAIALYVLGPVLIAYAFING
                     TSDGAGGTDPVWSLFVGGCVALGVAILLSPGFFTIQPNEARVLILFGAYHGTCKQVGF
                     RWGNPFYANGPAGQGSSAQPQWSWGKKDSGEGQGQGGPKKLKRYKVSLRARTLNGDRL
                     KVNDKRGNPVDIAAVVVWRVDDTAKAVFDVDDFETYVATQSETAVRHLASAYPYDSGE
                     TAADAEDEITLRGNVDEVSEALRVELTERLGQAGVVVEQARLSHLAYSPEIAQAMLRR
                     QQAEAVIAARRKIVTGAVSMVEMALDEFKEHQTIEFDEERKAAMVSNLMVVLCGESQV
                     TPVVNTGTLYT"
     gene            1626..2975
                     /locus_tag="VFZ86_02030"
     CDS             1626..2975
                     /locus_tag="VFZ86_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641098.1"
                     /translation="MLSTCAVALTVLAAVPAVAGAVPPQYPSGWTFAMNGLRQVRASV
                     DTRAFCKGPSDTVYEVALGQTTAYYASIARIRVSDGAVVKSWTYPANPGIGVIPRAAA
                     TDASGNLIVAAETLTGTRDWKVVKFSKSGTRIWKRTYDSGRGSDTPYGIVVDHHGGVI
                     VTGTSERTGGYDGAVVKWSSSGRLVWKRAISGPGLDLIGAVAVDANDNVYAAGDRSAA
                     GTWGTAILRSWTPGGRQRWTATSVNPLGTPAWRHLVVKGTSVYVAGQSTSVPSTSSLT
                     AMRYTTSGKRSWSGLRLFTFASGAWAEGLAVDRTGAPVIAGVAYDIGMSGEDQGVVWK
                     LTAAGDTAWHSEFEDIDFPHDGSFQAVGVDSKNRIYAAGGKYYSAQTANLLMVRYSPA
                     GSAQAMWRSDGQQSGYCSFTDLLVLSDTQVLAAGQVQGVLGSVGADACVYRAKTTSP"
     gene            3096..4412
                     /locus_tag="VFZ86_02035"
     CDS             3096..4412
                     /locus_tag="VFZ86_02035"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5641099.1"
                     /translation="MEPPVVAVPGAAGAAAPRLPRYRFVTLGVVWSAYLVVFLSRLCV
                     GPLAPFLKEAFDLSNAQIGGLTSATAVAYAPTLIFAGWLVDRIGVRRALLTGTVVTGL
                     CIGAVALAPSYGTMLLLLGASGLGCGFIYPTAVKAIMLWFPADERATAVGINQSAVNV
                     SGMLGAAIMPWLAVTFSWRAGFVFAAGLAFVVCVVAVLFYRDPRPGESPGAAQAPVPV
                     SEIDQGLPAVTGDAEWDQAVVKTGFAAVLLSRDIWMMGLAALFLGVVEFAALAHLVLF
                     LNSDWGYSVAVAAGMLAFCQFAGAIGKPLSGLVSDRALRRRRVPALLAMGALAGLSCV
                     AFALLQPGHTWLLWGALFLLGFGAVGWGGLMGTLAGETGGQRAAGAAAGVSAAFFNVG
                     IFLGPPIFGAIVDRTGFYAPAWWTLAASAAVALVLLLFVREGRGLV"
BASE COUNT          583 a         1620 c         1689 g          725 t
ORIGIN      
        1 gaggaggcgg ggaggaggcg gcggcgggcg tcgggccccg ccaccgcctc gtttccccgc
       61 cggtcagctc gtccgcgtgc ggccggatgg gccgccgccg cgcccgccgc tcgcggcggc
      121 gtccccggcc gcccggccgc cgcgccgccg ccgcaccgcg tcgcgcagga tgtactccac
      181 ctgcgcgttc acgctgcgga gctcgtcggc ggcccacctc tcgacctcgg cccacagggc
      241 ggggtcgacg cgcagcaggt actgtttgcg cgcagccatc tgctagcgcc gcctcacgga
      301 ccctgcgatc acgtgtagag ggtgccggtg ttgacgaccg gggtgacctg gctctccccg
      361 cagagcacga ccatcaggtt gctgaccatg gccgccttgc gctcctcgtc gaactcgatg
      421 gtctggtgct ccttgaactc atcgagcgcc atctcgacca tgctcaccgc gccggtgacg
      481 atcttgcggc gcgcggcgat cacggcctcg gcctgctggc ggcggagcat cgcctgggcg
      541 atctcggggg agtaggcgag gtggctgagg cgggcctgct cgaccacgac gcccgcctgc
      601 ccgagccgct cggtgagctc cacgcgcagg gcttcggaga cctcgtccac gttgccgcgc
      661 agggtgatct cgtcctcggc gtccgcggcg gtctcgcccg agtcgtaggg ataggcgctg
      721 gcgaggtgcc tgaccgccgt ctcgctctgg gtggccacgt acgtctcgaa gtcgtccacg
      781 tcgaacaccg ccttggccgt gtcgtcgacg cgccacacca cgacggcggc gatgtccacg
      841 ggattgccgc gcttgtcgtt gaccttgagc cggtcgccgt tgagggtgcg ggcgcgcagg
      901 ctcaccttgt agcgcttgag cttcttgggg ccgccctgcc cctgtccttc tcccgagtcc
      961 ttcttgcccc acgaccactg cggctgcgcg ctgctgccct gccccgcggg cccgttcgcg
     1021 tagaaggggt ttccccaacg gaagccgacc tgcttgcagg tgccgtgata ggcgccgaag
     1081 aggatcagca cgcgcgcctc gttgggctgg atggtgaaga acccggggct cagcaggatc
     1141 gccacgccga gtgcgacgca gccgccgacg aacagactcc acaccgggtc ggtgccgccg
     1201 gcgccgtcgg acgtgccgtt gatgaacgcg tacgcgatga ggaccggtcc gaggacgtac
     1261 agcgcgatgg cgaggacgag catcggccat ccgctcatca cgcgagcctc gcgctccttc
     1321 gtcggggtct tctgatccgc tgccatggct cccacctccg ggaggccgcg ccggcgggcc
     1381 ttgtcccgcc ccgagtgtcg cgcggccgct tgtgcgatac cgatgtgata tcacataggt
     1441 atcgctctgt cgagcggcgg cgccgcgacg ccgtcgcctg gcgcgacgac ggtcgtacgt
     1501 ggaaaagcat tgcctgcgtg cacgctccgc gacatactca gggcgatggg caacgcggcg
     1561 tgagcgccgg agtgggaccg cgcgtgcagc gcggccgaga gggggagacg atgagacttg
     1621 gccacatgct gtcaacgtgc gcggtcgcgc tgacggtcct cgccgccgtg cctgcggtcg
     1681 ccggggcggt gccgccacag tatcccagcg gctggacgtt cgcgatgaac ggcctgcggc
     1741 aggttcgggc ctcggtcgac acacgcgcgt tctgcaaggg gccgtcggac acggtgtacg
     1801 aagtggccct tggccagacg accgcctact acgcgtccat cgcacgaatc cgtgtgtcgg
     1861 acggcgccgt cgtcaagtcg tggacctacc ccgcgaaccc ggggatcggc gtcatcccgc
     1921 gagcggccgc gaccgacgcg tcggggaacc tcatcgtcgc tgcggagacc ttgaccggga
     1981 ccagggactg gaaggtggtc aagttctcga agtccgggac gcggatctgg aagaggacgt
     2041 acgacagcgg cagaggatcg gacacaccct acggcatcgt cgtcgaccac cacggcggcg
     2101 tgatcgtcac cgggaccagc gagcggaccg gggggtacga cggcgccgtc gtgaagtgga
     2161 gttcctccgg caggctggtc tggaagcgcg cgatctccgg gccggggctt gacctgatcg
     2221 gggccgtggc cgtggacgcg aacgacaacg tctacgccgc cggggacagg tcggcagcgg
     2281 gcacctgggg cacggcgatc ctgcgctcgt ggacgccggg cgggcgccag cgctggacgg
     2341 cgacgtccgt caatccgttg ggcaccccgg cctggcgtca cctcgtcgtc aagggcacgt
     2401 cggtgtacgt cgccggtcag tcgaccagcg tgccctcgac cagcagcctg acggcgatga
     2461 ggtacacgac gtcgggcaag cgctcgtgga gcggcctcag actgttcacc ttcgccagcg
     2521 gcgcgtgggc cgagggcctc gccgtggaca ggacgggtgc accggtgatc gccggcgtcg
     2581 cgtacgacat agggatgtcc ggcgaggacc agggcgtcgt ctggaagctc acggccgccg
     2641 gcgacaccgc gtggcacagc gagttcgaag acatcgactt cccgcacgat ggttcgttcc
     2701 aggccgtcgg ggtggacagc aagaaccgca tctatgccgc cggcggcaag tactacagcg
     2761 ctcagacggc gaacctcctg atggtgcgct actcgcctgc agggtcggcg caggcgatgt
     2821 ggcgctcgga cggccaacag agcggctact gctccttcac cgacctgctg gtgctgagcg
     2881 acacacaggt gctcgccgcc ggtcaggtcc agggcgtgct cggcagcgtc ggagccgacg
     2941 cgtgcgtgta ccgggcgaag acgaccagcc cctgacgtga gtcgctgagg gcggcggccc
     3001 ctgatcggcg gctgccgcgc cgcgtgaacc aggccgaggc gaagcggcgg tccggggcga
     3061 cgccggccgg ccggcgcacc agctaggatc gtggcatgga accacccgtc gtagccgtcc
     3121 cgggcgcggc cggcgcagcc gcgccgcgcc tcccgcgcta ccgcttcgtc acgctgggcg
     3181 tggtgtggtc cgcgtacctc gtcgtcttcc tctcgcgcct gtgcgtgggg ccgctggcgc
     3241 cgttcctcaa ggaggcgttc gacctcagca acgcccagat cggcggcctg accagcgcga
     3301 ccgccgtcgc gtacgccccc actctgatct tcgccgggtg gctcgtggac cgcatcggcg
     3361 tgcgccgcgc gctgctcacc ggcaccgtcg tcaccggctt gtgcatcggg gcggtggcgc
     3421 tggcgccgag ctacgggacg atgctcctgc tgctcggcgc ctccggcctc ggctgcgggt
     3481 tcatctaccc gacggccgtc aaggcgatca tgctgtggtt cccggccgac gagcgggcta
     3541 cggccgtggg gatcaaccag tcggccgtca acgtcagcgg catgctgggc gcggcgatca
     3601 tgccgtggct ggccgtgacg ttcagttggc gggccgggtt cgtcttcgcc gccggcctcg
     3661 cgttcgtcgt gtgcgtggtc gccgtgctct tctaccgcga cccgcggccg ggggagtcgc
     3721 cgggggcggc gcaggcgccc gtcccggtgt ccgagatcga ccaggggctg ccggcggtga
     3781 ccggcgatgc ggaatgggac caggcggtgg tcaagaccgg gttcgccgcc gtcctcctct
     3841 cgcgcgacat ctggatgatg gggctcgcgg cgctcttcct cggggtcgtc gagttcgccg
     3901 cgctcgccca cctcgtgctg ttcctgaaca gcgactgggg ctacagcgtg gccgtggccg
     3961 ccggcatgct ggccttctgc cagttcgccg gcgccatcgg caagccgctc agcggcctcg
     4021 tgagcgatcg cgcgctgcgc cgccgcaggg tgccggcgct gctggccatg ggtgcgctcg
     4081 ccggcctctc gtgcgtcgcg ttcgcgctgc tccagcccgg ccacacgtgg ctgctgtggg
     4141 gcgcgctgtt cttgctcggg ttcggcgccg tcggctgggg aggcctgatg ggcacgctcg
     4201 ccggagagac cggcggacag cgcgccgccg gcgccgcggc cggcgtctcg gcggcgttct
     4261 tcaatgtcgg catcttcctg gggccgccga tcttcggcgc catcgtggac cgcacagggt
     4321 tctacgcgcc ggcctggtgg acgctggcgg cgtcggcggc cgtggcgctc gtgctgctgc
     4381 tcttcgtccg cgagggccgc ggcctggtct gacacgcgtc agggggcgca gggtggccga
     4441 ccggcagcct taccgcgcgg ccgggtccgc aagcgaagag gggcgcctct gcgaggcgcc
     4501 cctcttcgcg tcgagtggcc ggcgccggcg gctcgcgccg ccggccgagc cggcggttca
     4561 catactggtc aggctctcga tctgaggcgt caccacctcg agcgtgtccg ccatgcc
//
LOCUS       DASUMX010000047         7311 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_731278, whole genome shotgun sequence.
ACCESSION   DASUMX010000047 DASUMX010000000
VERSION     DASUMX010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 7311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7311
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_731278"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /gene="tsaB"
                     /locus_tag="VFZ86_02040"
     CDS             complement(<1..490)
                     /gene="tsaB"
                     /locus_tag="VFZ86_02040"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEX5641100.1"
                     /translation="MILALETATTACSAALCAADGSVVAERLALDGPAHSRLLLPFVH
                     EILGETGAGWDDIETVAVGLGPGAFTGLRIGIATARALAQADGRAGLAGVPTLTALAL
                     ALAGAPEAAPGRLLVPLVDGRRREVFAAVFEITGVGLRQIEDVTVVAADRLAAWLAAR
                     GEA"
     gene            complement(487..981)
                     /gene="tsaE"
                     /locus_tag="VFZ86_02045"
     CDS             complement(487..981)
                     /gene="tsaE"
                     /locus_tag="VFZ86_02045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEX5641101.1"
                     /translation="MIALTLASPAQTRKLARALAVMLRPPALITVEGDLGTGKTTLVR
                     EVLRARGVRGAITSPSFTLAQSYHGRGGELLHHLDLYRLSRGVDAELFAWDDYLGPDA
                     ITFVEWPAAGGDALPPADLRIELEHRTPNSRTARLSGEPALEADVAAAMTGAGVRVDA
                     RSAP"
     gene            complement(982..1920)
                     /locus_tag="VFZ86_02050"
     CDS             complement(982..1920)
                     /locus_tag="VFZ86_02050"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX5641102.1"
                     /translation="MAPATDEVIAALDALREEVERCSSCVLAESRTRAVFGEGDPGAD
                     LMFVGEAPGYHEDQQGRPFVGQAGKLLEQLLASIGMTREQVFIANVLKSRPPNNRDPR
                     PEEIDACRPYLWRQIEIIKPKVICTLGNFATKLLSGDQTGITKVHGRPRPTEIAGHRL
                     YLYPIFHPAAALYTPAMLTTLKEDFSRLPQLLAHAAPQPGEEAAQPPAAERPAWTPPK
                     HLAGGRDDGEDLRDGSLSLDDVAAVAGASVAAVADPPPVSAGPATPAPVVHELAATDA
                     APPIAPEPATPEPATPEPATPPEAGPPAEPEQLGLF"
     gene            complement(1904..2122)
                     /locus_tag="VFZ86_02055"
     CDS             complement(1904..2122)
                     /locus_tag="VFZ86_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX5641103.1"
                     /translation="MPIYEFCCNQCGTAFEELVRNGTRPECPECHSGDVCRLLSTFAV
                     HSSSSNLGGKGATKNCSSCASSSCGTCH"
     gene            2371..3333
                     /locus_tag="VFZ86_02060"
     CDS             2371..3333
                     /locus_tag="VFZ86_02060"
                     /inference="COORDINATES: protein motif:HMM:NF022188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rossmann-like and DUF2520 domain-containing
                     protein"
                     /protein_id="HEX5641104.1"
                     /translation="MTLDAYRFCVIGPGRLGSAVLAGLQEAELTVSAVGVHGDDDTDL
                     FGAVPHVRVRDAALEADAWWLAVPDDEIYAVAGRLAEALPAIRRAGAPQPLALHSSGL
                     GSVHLLKPLYEQGVRVLCLHPLQTFAGEPSAELLRGVPCAVTALEDRDLQLGEELAGR
                     LGMRPFRLADEQKTLYHLAAVVGCNLLVALESEAKRLMDEATGETRGLDHLSVLLETT
                     LRNLLQSGEPAFALTGPVARGDIGTVRAHLRLLDRESPRLARTYRALSLEALALAAPR
                     LDDEQVETLRELLGAGVAGGGAAGPAGPEGAAPATGSHWAGGQP"
     gene            3330..4241
                     /gene="panC"
                     /locus_tag="VFZ86_02065"
     CDS             3330..4241
                     /gene="panC"
                     /locus_tag="VFZ86_02065"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEX5641105.1"
                     /translation="MIVARTIAEARAALRMLPRPLGFVPTMGSLHAGHLSLVDAAKGR
                     CSSVAASVFVNPTQFGVGEDFTAYPRDEARDLHLLEKAGVAVVFAPDADEMYPDGFAT
                     VVHVGGRLTETFEGASRPSHFDGVAVVVTKLLGVVQPDVLLLGEKDAQQLAVVRRFVR
                     DLDLQVEVVGVPTLRETDGLAMSSRNLFLTAEQRAVAPDLYRALLAGVAAAGAPGADT
                     KDVVAAATSALLLPDGALDPDGIDEALARPRFELDYLAVVDEDTFVPEGTLGPRSRLV
                     VAARLGSTRLIDTIKLDSAYPAKDATA"
     gene            4238..4729
                     /locus_tag="VFZ86_02070"
     CDS             4238..4729
                     /locus_tag="VFZ86_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014405997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HEX5641106.1"
                     /translation="MRERTTMATVFVNGKQQDHVREGLVRVITGSIEFPSNGDADMIK
                     ITDEVKEAVRRAGLTDGTVTLFVPGATGALTTLEYEPGVVEDVQRALDVVAPADRFYQ
                     HNANLGDGNGHSHVRAGLVGPSLVVPFVDGRLTLGIYQNIVFCDFDARPRERSIVVQV
                     MGV"
     gene            4726..6045
                     /gene="hxlA"
                     /locus_tag="VFZ86_02075"
     CDS             4726..6045
                     /gene="hxlA"
                     /locus_tag="VFZ86_02075"
                     /EC_number="4.1.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010878362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043801 - hexulose-6-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019647 - formaldehyde assimilation via
                     ribulose monophosphate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hexulose-6-phosphate synthase"
                     /protein_id="HEX5641107.1"
                     /translation="MNGADCDPSRERPLVQVALDFVDLPRALEVAREAVAGGADWIEA
                     GTPLIKAEGLAAVRALKAEFPERTIVADMKTMDAGRTEVEYAAKAGAGVVGVLGAASD
                     STIKECAEAARNYGCKLIVDMIEVADPVARARRAQELGADYIGIHTAIDQQMRGEAPF
                     ETLKAVAEAVVIPVSVAGGINSETAAAAVDAGAGIVVVGGAIIKSADAAAATAEIRRA
                     VDERVCIETTLYKRAGAADIRAALEQVSTANVSDAWHRQPSLPHIRPLLPGAHMCGPA
                     VTVRTYAGDWAKPVEAIDVCKPGDVLVVDACDATPAMWGELATHSAMVKGVAGLVVWG
                     AIRDTPEIARLGFPAFSSKVCANAGEPKGFGEINVPVTIAGQTVRPGDWIVGDDDGVM
                     VLPKEQAVELANRAMDVLEKENRLRSEIDAGKTLAEVAYLQKWEKAR"
     gene            6546..>7311
                     /locus_tag="VFZ86_02080"
     CDS             6546..>7311
                     /locus_tag="VFZ86_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641108.1"
                     /translation="MSAAAASDGAAPKRLSGLEQRAENLHRKIDRLAQRSGKVKRSPR
                     LRGKVLRTNALVKRMNRGLIRLGEVVLTASEVDDAFVAEVLACNAAAAHLDRDAIRLS
                     RRTLKKARSRAVSSLSQARTEIIALSAKLGRPKKPSTPAPTPTATVTPTPTAAPAPTA
                     PAPTTGNPISTINNATSGQTVVCPAGTFTGDVRVPSGVTVIGQGMDRTRIVGSLTWGS
                     NTTVRDLAVGGKGHSHEPATYTTSNTRFERVRFFGGG"
BASE COUNT         1054 a         2666 c         2610 g          981 t
ORIGIN      
        1 gcgcctcgcc gcgcgcggcg agccacgcgg ccagccggtc ggccgcgacg acagtgacgt
       61 cctcgatctg ccgcaggccg acgccggtga tctcgaagac ggccgcgaac acctcgcggc
      121 ggcggccgtc gaccagcggc accagcagtc gtccgggcgc cgcctcgggc gcgccggcca
      181 gcgcgagcgc cagggccgtg agcgtgggca ccccagccag gccggcccgg ccgtctgcct
      241 gcgccagcgc ccgcgcggtc gcgatgccga tgcgcagccc ggtgaacgcg cccggtccaa
      301 gaccgacggc gacggtctcg atgtcgtccc agccggcgcc ggtctcgccg aggatctcgt
      361 gcacgaaggg cagcagcagc cgtgaatgcg cggggccgtc gagcgccagg cgctcggcga
      421 cgacggagcc gtcggcggcg cacagcgccg ccgaacacgc cgtcgtcgcc gtctccagcg
      481 cgaggatcac ggcgcggacc tcgcgtccac cctgacgccg gcgccggtca tcgccgccgc
      541 gacgtccgcc tcgagcgccg gctcccccga cagccgcgcc gtgcggctgt tgggcgtccg
      601 gtgctccagt tcgatacgga ggtcggccgg cggcagcgcg tcgccgccgg ccgccggcca
      661 ctcgacgaac gtgatcgcgt cgggcccgag gtagtcgtcc cacgcgaaca gttccgcgtc
      721 gacgccgcgg ctgagccgat acaggtcgag gtggtgcagg agctccccgc ctcgcccgtg
      781 gtagctctga gcgagcgtga acgagggact ggtgatcgca ccgcgcacgc cgcgggcgcg
      841 cagcacctcg cggacgagcg tcgtcttgcc ggtgccgagg tcgccctcca ccgtgatcag
      901 cgccggcggg cgcaacatca cggccagcgc acgcgcgagc ttccgcgtct gggccggcga
      961 ggcgagagtg agcgcgatca tctagaagag gccgagctgc tccggttcgg ccggcgggcc
     1021 ggcttcggga ggcgtcgccg gctcgggcgt cgccggctcg ggcgtcgccg gctcgggcgc
     1081 gatcggcggg gcagcgtccg ttgccgccag ctcgtgcacg accggcgccg gtgtcgccgg
     1141 ccccgcggag acgggtggcg ggtcggccac ggccgcaacg gaggcgccgg ccaccgctgc
     1201 gacgtcgtcg agcgagagcg agccgtcgcg cagatcttcg ccgtcgtcac gcccgcctgc
     1261 gaggtgcttg ggcggcgtcc aggccggccg ctccgcggcc ggcggctgcg ccgcctcctc
     1321 gcccggctgc ggcgccgcgt gcgccagcag ctgcgggagc cgggagaagt cctctttgag
     1381 cgtcgtcagc atcgccggcg tgtagagcgc cgcggcggga tggaagatcg gatacaggta
     1441 cagccggtgc ccggcgatct cggtcgggcg cggacgcccg tgcaccttgg tgatgccggt
     1501 ctggtccccg ctgaggagct tggtcgcgaa gttgcccagc gtgcagatga ccttgggctt
     1561 gatgatctcg atctgccgcc acaggtacgg gcggcaggcg tcgatctcct ccggtcgcgg
     1621 atcgcggttg ttgggcgggc ggctcttgag gacgttggcg atgaacacct gctcgcgggt
     1681 catgccgatg gacgcgagca gctgctcgag gagcttgccg gcctggccga cgaacggccg
     1741 gccctgctgg tcctcgtggt agccgggcgc ctctccgacg aacatgaggt cggcgccggg
     1801 gtcgccctcg ccgaacaccg ccctggtgcg cgactccgcg agcacgcagc tggaacagcg
     1861 ctctacctcc tcgcgaaggg cgtcgagcgc ggcgatgacc tcgtcagtgg caggtgccac
     1921 aggaggagct ggcgcaggag gagcagttct tggtcgctcc tttgccgccg aggttggacg
     1981 acgatgagtg gacggcgaac gtggacagca gccggcagac gtcgccggag tggcactcgg
     2041 ggcactcggg cctggtgccg ttgcggacca gctcctcgaa ggcggttccg cactggttgc
     2101 agcagaattc atagatcggc atggcggtag tgtaccaccg gaggccggca ggcagacgct
     2161 gggcggtgcg gcgcggaggg gcagtggcgt cacggtccgc accgtcacaa cgaccgtccg
     2221 attgacacgc gcacgcgcga gaccctacgc tttgagatgg cccggcgccg agcctcggca
     2281 ggccaccggg acaccgcctg tgctgcacag gcctacggca ggcgcggtac ctcccccacc
     2341 gggcggaccg cagccggaga ggtcggaggc ttgaccctcg acgcgtatcg cttctgcgtg
     2401 atcggtcccg gacgcctggg atcggccgtc cttgccggcc tgcaggaggc cgagctcacc
     2461 gtgagcgcag tcggcgtcca cggcgacgac gacaccgacc tcttcggcgc cgtaccccac
     2521 gtgcgcgtgc gcgacgccgc gctggaggcg gacgcctggt ggctggcggt gccggacgac
     2581 gagatctatg cggtggccgg ccgcctggcg gaggcgcttc cggccatccg ccgcgccggc
     2641 gcgccccagc cgctcgccct gcactccagc ggcctcggtt ccgtgcacct gctcaagccc
     2701 ctgtacgagc agggcgtgcg cgtgctctgc ctgcacccgc tgcagacgtt cgccggcgag
     2761 ccgagtgcgg agctgctgcg aggcgtcccc tgcgccgtca cggcgctcga ggatcgtgac
     2821 ctgcagctcg gggaggagct cgccggccgt ctcggcatgc gcccgttccg tctggccgac
     2881 gagcagaaga cgctgtatca cctcgccgcc gtcgtcggct gcaacctgct cgtggccctc
     2941 gagagcgagg ccaagcggct catggacgag gccaccggcg agacacgcgg cctcgaccac
     3001 ctctccgtgc tcctcgagac gaccttgcgc aacctgctcc agagcggtga gcccgcgttc
     3061 gcgctcacag ggccggtcgc gcgcggtgac atcggcaccg tgcgcgcgca cctgcgcctg
     3121 ctcgatcgcg agagcccgcg gctggcgcgg acgtaccgcg ctctcagcct ggaggcgctg
     3181 gcgctggccg caccgcggct cgacgacgag caggtcgaga ctctgcgaga gctgctcggg
     3241 gccggggtag ccggcggcgg agccgccggc cccgccggcc cggagggcgc cgcgccggcg
     3301 accgggagcc actgggcggg aggccagccg tgatcgtcgc ccgcaccatc gccgaggcgc
     3361 gggcggcgct gcgcatgctg ccgcggcccc tcggattcgt gcccacgatg ggttccctgc
     3421 acgcggggca cctgtccctg gtggacgccg cgaaaggacg ctgctcgagc gtcgccgcct
     3481 cagtcttcgt caaccccacg caattcggcg tcggcgagga cttcacggcg tacccgcggg
     3541 acgaagcccg cgacctgcac ctgctcgaga aggcgggggt cgcggtcgtc ttcgcgccgg
     3601 acgccgacga gatgtacccg gacggcttcg ccaccgtggt ccacgtcggc ggaaggctca
     3661 ccgagacctt cgagggcgcc tcacgcccga gccacttcga cggcgtcgcc gtcgtcgtca
     3721 ccaagctgct cggcgtggtg cagccggacg tgctgctcct cggcgagaag gacgcgcagc
     3781 aactcgccgt cgtgcgccgc ttcgtccgcg acctcgacct gcaggtcgag gtcgtcggcg
     3841 tgccgacgct gcgcgagacg gatggcctcg ccatgagctc gcgcaatctc tttctcactg
     3901 cggagcagcg cgcggtggcg cccgacctct accgcgcgct cctcgccggc gtggccgccg
     3961 caggcgcgcc cggcgcggac accaaggacg tcgtcgccgc ggcgacgagc gccctgctgc
     4021 tcccggacgg agccctcgac cccgacggga tcgacgaggc tcttgcccgt ccgcgcttcg
     4081 aactcgacta cctcgccgtc gtggacgagg acacgttcgt ccccgagggc acgctggggc
     4141 cgcgatccag gctcgtcgtc gcggcccggc tcggctccac ccgcctcatc gacaccatca
     4201 agctggacag cgcgtacccc gccaaggacg cgaccgcgtg agagaaagga ccaccatggc
     4261 gaccgtcttc gtcaacggca agcagcagga ccacgtgcgc gagggcctcg tgcgggtcat
     4321 caccggctcc atcgagttcc ccagcaatgg cgacgccgac atgatcaaga tcaccgacga
     4381 ggtcaaggag gccgtgcgcc gggctggcct gaccgacggc accgtgaccc tgttcgtccc
     4441 cggcgccacc ggcgcgctca cgacgctcga gtacgagccc ggcgtggtgg aggacgtgca
     4501 gcgggcgctc gatgtggtgg cccccgccga ccgcttctac cagcacaacg cgaacctcgg
     4561 cgacggcaac ggccactcgc acgtgcgcgc cgggctcgtg gggccgtcgc tggtcgttcc
     4621 cttcgtcgac gggcgcctca ccctcgggat ctaccagaac atcgtcttct gcgacttcga
     4681 cgcgcgcccg cgcgagcgct cgatcgtggt gcaggtgatg ggcgtatgaa cggcgccgac
     4741 tgcgacccga gcagagagcg cccgctcgtc caggtcgcgc tcgacttcgt cgaccttccg
     4801 cgggcgctcg aggtcgcacg cgaggccgtc gccggcggcg ccgactggat cgaggccggc
     4861 acgccgctca tcaaggccga gggcctggcc gccgtgaggg cgctgaaggc cgagttcccg
     4921 gagaggacca tcgtcgctga catgaagacg atggacgccg gccgcaccga ggtggagtac
     4981 gccgccaagg ccggagccgg ggtggtcggc gtgctgggcg ccgcgtccga cagcacgatc
     5041 aaggagtgcg ccgaggcggc gcgcaactac ggctgcaagc tgatcgtcga catgatcgag
     5101 gtcgccgacc cggtggcgcg cgcgcggcgc gcgcaggaac tcggcgccga ctacatcggc
     5161 atccacaccg ccatcgacca gcagatgcgc ggcgaggcgc cgttcgagac gctcaaggcc
     5221 gtcgccgagg cggtcgtcat ccccgtctcg gtggccggcg ggatcaacag cgagacggcg
     5281 gctgcggccg tcgacgccgg cgccggcatc gtcgtcgtcg gcggcgccat catcaagtcc
     5341 gccgacgcgg cggcggccac cgccgagatc cgccgcgccg tggacgagcg cgtctgcatc
     5401 gagaccacgc tgtacaaacg cgccggcgcg gcggatatcc gcgcggcgct ggagcaggtc
     5461 tcgacggcca acgtcagcga cgcctggcac cggcagccga gtttgccgca catccggcct
     5521 ctgctgccgg gtgcgcacat gtgcggcccc gcggtcaccg tccgtaccta tgccggcgac
     5581 tgggcgaagc ctgtggaggc gatcgacgtc tgcaagccgg gcgacgtcct cgtcgtcgac
     5641 gcctgcgacg cgacccccgc catgtggggc gagctcgcca cgcacagcgc gatggtcaag
     5701 ggtgtcgcag ggctggtcgt gtggggcgcg atccgggaca cgccggagat cgcgcgcctc
     5761 ggcttccccg ccttcagcag caaggtctgc gccaacgccg gcgagcccaa gggcttcggc
     5821 gagatcaacg taccggtcac catcgccggg cagacggtgc gtcccggcga ctggatcgtc
     5881 ggcgacgacg acggcgtgat ggtcctcccc aaggagcagg ccgtggagct cgccaaccgt
     5941 gccatggacg tgctcgagaa ggagaaccgc ctgcgcagcg agatcgacgc gggcaagacg
     6001 ctcgccgagg tcgcctacct gcagaagtgg gagaaggcgc gctaggcacg ccttgccgag
     6061 tgatctggcg gctgaccgac gcaggcggga gagggtcgtc atcggcgtcg ccaggcctgg
     6121 ggaacagcac cgcgaagcgg taggtccccg tctccttggg acgcaggttg ccgagcctgg
     6181 cgaggggcac ctgctgcagt aggctggagg gggtgagctc cggccgcctc gtcgacacca
     6241 gaccgcaccg cgtaggcaga ggctcttcga ctgccacctt gaccgtacac aattccggag
     6301 acacttgtcg gcggcctgcc ctcagttccc gctggatcgt gccgaccctt caagtgtcac
     6361 aggcctcggc cggggcctaa tcccaggaat cgatcccagc gtgatcgcac ccgtgcgccc
     6421 gggggctttg ctgtgcgccg atcggccccc tctcaccttg ggatcagcag gaggcacgag
     6481 tgtccaggtt gaagacatcc cccgcggtca tcaccggcgt cctcatcatc agtctgctcg
     6541 tccccatgtc cgcagcggcc gcgtctgatg gcgcggcgcc caagcgcctc tccgggctcg
     6601 aacaacgcgc agagaacctg cacaggaaga tcgacaggct ggcgcagagg tccggcaagg
     6661 tgaagcgttc gcctcgcctc cgcggcaagg tcctcaggac caacgccctc gtgaagcgca
     6721 tgaaccgcgg cctcattcgg ctcggcgaag tcgtgctgac ggcttctgag gtggacgacg
     6781 cgttcgtggc cgaggtgctc gcctgcaacg ccgccgcagc ccacctcgac agggatgcga
     6841 tccgcctctc ccggcgcaca ctcaagaagg cccgcagccg tgcggtcagc tccctctcgc
     6901 aagcacgcac ggaaatcatc gcgctctcgg ccaagcttgg ccggccaaag aagccgtcga
     6961 cacctgcccc cacgccgacc gcgaccgtga ccccgacccc gacggccgcg ccggcgccga
     7021 ccgcccccgc cccgacgacg ggcaacccca tctcgaccat caacaacgcg accagcggcc
     7081 aaacggtggt ctgcccggca ggcacgttca cgggtgacgt ccgggtgccc agcggtgtga
     7141 ccgtcatcgg acagggcatg gaccggaccc gcatcgtcgg ctccctcacc tggggctcga
     7201 acaccaccgt ccgcgacctc gccgtgggcg ggaaggggca ctcgcacgag cccgcgacct
     7261 acacgacctc caacacccgt ttcgagcgtg tccgcttctt cggcggtggc g
//
LOCUS       DASUMX010000048         4017 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1162826, whole genome shotgun sequence.
ACCESSION   DASUMX010000048 DASUMX010000000
VERSION     DASUMX010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4017
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1162826"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            217..654
                     /gene="dtd"
                     /locus_tag="VFZ86_02085"
     CDS             217..654
                     /gene="dtd"
                     /locus_tag="VFZ86_02085"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015234307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HEX5641109.1"
                     /translation="MRVLLQRVSRAAVAVDGAETGAVGRGFLALVGVTHEDDATAARR
                     LAAKTARLRVFADDAGLMNLALGDVGGAVLAVSQFTLYADVRRGNRPSFTDAAPPDRG
                     EAVYEAYVEALRAEGVPVETGVFGAHMQVELVNDGPVTILLEA"
     gene            complement(736..1173)
                     /locus_tag="VFZ86_02090"
     CDS             complement(736..1173)
                     /locus_tag="VFZ86_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641110.1"
                     /translation="MKGQRYANLTGVTGIIAGAFAIGGGLYARSIAPDFGIPQFFGIY
                     SDYLSGVHVLVGFGIVMVIGGLLSFKWPSVGGVIVCLAAMIGLIYTYNRGQYRWTPYL
                     YYWWGPWLFAWICGIFAGYAAYKNVPQSGEKLTDARHAEGYEV"
     gene            complement(1170..2594)
                     /locus_tag="VFZ86_02095"
     CDS             complement(1170..2594)
                     /locus_tag="VFZ86_02095"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX5641111.1"
                     /translation="MTELLRNEGLKRVRMRLITAVIFVFTLTCSGSFGMEDVVYSSGP
                     GLTLLMILILPIVWSVPMAFVASELGSMVPEAGGLYRWIRRGMGEYWSFQAGWWWTLS
                     LYVDSAVYVALALDYMQNQWGFGATERALIGVGIVAVFTFINIRGLELTGWSLTIIQV
                     GVMVPLLLFTVWGIIEGSGNPFSPMMYEGESLLTSLNLGLAVMMWMYSGWESMSTLAG
                     EIQNPQRIIPKALMIGTPLVIATYFITVYAALRVANLGGPDNWQNMWTGGGGQDFVQL
                     AGVVGGTFLTYLVLMSAILSNIGLYAGYLATGSRPQFQMSRDRLLPGFIGITHKSWGT
                     PWVAILMMGVVNAVLINFEFDALITIDVFLLMFPYVLIFLTVMIMRVREPNTPRSFRV
                     PLPTWALGVWVAFPIAIAIIALFVNGMDWMIGGLAGILTGPIAYLVFKNIYKGTTDEA
                     LEGSTITPTGELTEFGAAVEGGQA"
     gene            complement(2626..>4017)
                     /locus_tag="VFZ86_02100"
     CDS             complement(2626..>4017)
                     /locus_tag="VFZ86_02100"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF006187.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HEX5641112.1"
                     /translation="TLDHAPLVALTGQTGLAGMHKETTHQFIDTVEMLHPMTKWNTRI
                     HHPRMVPEAVRKAFSIAAAEKPGATHLELPEDVMEMAVTGSPLPRGPEPMVEADPVSL
                     LRAAEVIQGAKRPVLLVGNGVVRQHAAAALRTLCEQTGLHAVTTFMGKGVLDAADPHF
                     LFTAGLRRQDYPQGLLGRADLVVSVGYDMIEWPPSAWNPDGGQRIICIDTVPPEIDAH
                     FIPEVELIGDLSRIIFQLTELLEGRKVASMEVRPYRRAIRRVLDAGTDDDFPVKPQRV
                     LRDLRELLAPEDVLVSDVGAHKLWVARFWEAREPNTVLISNGFAAMGFGLPATIAAAL
                     ARRGRGKVVCISGDGGFLMNVQELETARRLGLPFVILVWTDGGYGLIEMHQRRKYGRI
                     AGTRFDNPDLVALARSFGVEGLRAEKAGDVKPLLARALRHPGPVVIDVPIDYGENDKL
                     AVDLWQLAPEALS"
BASE COUNT          665 a         1320 c         1428 g          604 t
ORIGIN      
        1 ttgagagatg tcaaaccctg cggtgcctga ggagcaagcg ccctggtgac ggcgctggac
       61 atgggtgttt tgcaggcctt ttaccgtccg ccggcgacga tggcacgcct gtcagtccac
      121 ccacacgagt gccgccgggt cttcgccgct gtcgtatctg gcctccgccg gcccgcctcg
      181 ttaggattgc ggcacgatgg acgagcgcgt cgccgtatga gagtgctgct gcaacgggtg
      241 agccgtgccg cggtggccgt ggacggcgcg gagaccggtg cggtcggccg gggcttcctc
      301 gccctcgtgg gcgtcaccca cgaggacgac gcgaccgctg cccgcaggct cgcggccaag
      361 acggcgcggc tgcgcgtctt cgccgacgac gccgggctca tgaacctggc gctcggggac
      421 gtcggcggcg ccgtgctcgc cgtctcgcag ttcaccctgt acgcggacgt gcggcgcggc
      481 aaccggccga gcttcacgga cgccgcgccg ccggatcgcg gcgaggcggt ctacgaggcc
      541 tacgtcgagg cgctgcgcgc cgaaggcgtg ccggtcgaga ccggcgtgtt cggcgcgcac
      601 atgcaggtcg agctggtcaa cgacggcccc gtgacgatcc tcctggaggc ctgaccggga
      661 gccaagcccg gagacgacgg cgcccgggcc gcctgcaggc ggcccgggcg catgagcaca
      721 cggcgtgagc gcgcctcaga cctcgtagcc ctcggcgtgg cgcgcgtcgg tcagcttctc
      781 gccgctctgc ggcacgttct tgtaggccgc atagccggcg aagatgccgc agatccaggc
      841 gaacagccac gggccccacc agtagtacag gtacggggtc cagcggtact ggccgcggtt
      901 gtaggtgtag atgaggccga tcatcgccgc gagacagacg atgacgccgc cgacgctcgg
      961 ccacttgaag ctgagcaggc caccgatgac catcacgatc ccgaagccga ccagcacgtg
     1021 cacgccggag agatagtcgg agtagatgcc gaagaactgg gggatgccga agtccggcgc
     1081 gatggagcgg gcgtagagac cgccgccgat cgcgaaggct ccggcgatga tgccggtgac
     1141 ccccgtcagg ttggcgtacc gctgaccctt catgcctggc ctccctcaac ggcggcgccg
     1201 aactcggtga gctcgccggt cggcgtgatc gtcgaaccct cgagcgcctc gtcggtggtg
     1261 cccttgtaga tgttcttgaa gacgaggtag gcgatgggcc cggtgaggat gcccgccaga
     1321 ccaccgatca tccagtccat gccgttgacg aacagggcga tgatggcgat ggcgatgggg
     1381 aaggcgaccc acacgccgag cgcccacgtc gggagcggca cgcggaacga gcgtggcgtg
     1441 ttgggctctc tgacgcgcat gatcatgacc gtcaggaaga tgagcacgta cgggaacatg
     1501 agcaggaaca cgtcgatggt gatgagcgcg tcgaactcga agttgatgag cacggcgttg
     1561 acgacgccca tcatgaggat ggccacccac ggcgtgcccc agctcttgtg ggtgatgccg
     1621 atgaaccccg gcagcaggcg gtcgcgcgac atctggaact gcggccgcga gcccgtggcg
     1681 aggtagcccg cgtacaggcc gatgttggag aggatggccg acatcagcac caggtaggtg
     1741 aggaaggtgc cgccgacgac cccggcaagc tgcacgaagt cctggccgcc gcctccggtc
     1801 cacatgttct gccagttgtc gggtccgccc agattggcca cgcggagcgc cgcgtacacg
     1861 gtgatgaaat aggtggcgat caccagcggt gtgccgatca tgagggcctt ggggatgatc
     1921 ctctgcgggt tctggatctc cccggcgagc gtggacatcg actcccagcc ggagtacatc
     1981 cacatcatca ccgccaagcc caggttgagg gaggtgagca ggctctcacc ctcgtacatc
     2041 atcgggctga acgggttgcc gctgccctcg atgatgcccc agacggtgaa cagcagcagg
     2101 gggaccatga cgccgacctg gatgatcgtg agcgaccacc cggtcagctc gaggccccgg
     2161 atgttgatga acgtgaacac cgccacgatg ccgacgccga tgagcgctct ctcggtggcg
     2221 ccgaaccccc actggttctg catgtagtcg agggcgaggg ccacgtagac ggccgagtcg
     2281 acgtacagcg agagcgtcca ccaccagccc gcctggaagc tccagtactc gcccatgccc
     2341 cggcggatcc agcggtacag gccgccggcc tcggggacca tggacccgag ctccgaggcg
     2401 acgaacgcca tgggcacgct ccacacgatc ggcaggatga ggatcatgag gagcgtcagt
     2461 ccggggcccg acgagtagac gacgtcctcc atgccgaacg agccggagca agtgagcgtg
     2521 aagacgaaga tgaccgcggt gatcagccgc atccgtacgc gcttgagccc ctcgttacgc
     2581 agcagttccg tcatccggac cccccagagt acgtttgtac aagttctagg acagtgcctc
     2641 gggagcaagt tgccagaggt cgaccgccag cttgtcgttc tcaccgtaat ctatcggaac
     2701 gtcgatgaca acgggtccgg ggtgcctgag ggcgcgggcg aggagaggct tgacgtcgcc
     2761 ggccttctcg gcgcgaagcc cttccacgcc gaacgagcgc gcgagcgcca cgaggtcggg
     2821 gttgtcgaag cgggtgccgg cgatgcggcc gtacttgcgc cgctggtgca tctcgatcag
     2881 gccgtagccg ccgtcggtcc acacgaggat cacgaacggg aggccgaggc ggcgggccgt
     2941 ctccagctcc tggacgttca tgaggaagcc gccgtcgccg ctgatgcaca cgaccttgcc
     3001 gcggccgcgg cgcgccagcg ccgcggcgat cgtcgccgga aggccgaagc ccatggcggc
     3061 gaagccgttg ctgatgagca cggtgttggg ctcgcgcgcc tcccagaagc gcgccaccca
     3121 gagcttgtgc gcgccgacgt cgctgacgag cacgtcctcc ggcgcgagca gctcgcgcag
     3181 gtcgcgcagc acgcgctgcg gcttgaccgg gaagtcgtcg tcggtgcccg cgtcgagcac
     3241 gcggcggatg gcgcgccggt agggccggac ctccatgctc gccaccttcc tcccctccag
     3301 aagttccgtg agctggaaga tgatgcgcga gaggtcgccg atgagctcga cctcggggat
     3361 gaagtgggcg tcgatctcgg gcggcaccgt gtcgatgcag atgatgcgct gccctccgtc
     3421 ggggttccag gccgagggcg gccactcgat catgtcgtac ccgacgctga ccacgagatc
     3481 cgcccggccg aggaggcctt gaggatagtc ctgccggcgc agccccgcgg tgaagaggaa
     3541 gtgtgggtcg gcggcgtcga ggacgccctt gcccatgaac gtggtgaccg cgtgcaggcc
     3601 cgtctgctcg cacaaggtgc gcagggccgc ggccgcgtgc tggcgcacga caccgttgcc
     3661 cacgagcagc accggccgct tcgccccctg gatgacctcg gcggcgcgca gcaggctgac
     3721 ggggtcggcc tcgaccatcg gctccgggcc gcgcggcagc ggcgagccgg tcaccgccat
     3781 ctccatcacg tcctcgggca gctcgagatg cgtggcgccc ggcttctcgg cggcggcgat
     3841 gctgaacgcc ttgcggaccg cctcgggcac catgcgcggg tggtggatgc gggtgttcca
     3901 cttggtcatg ggatgcagca tctcgacggt gtcgatgaac tggtgggtgg tctccttgtg
     3961 catccccgcg agcccggtct ggcccgtgag cgccaccagg ggcgcgtggt cgagcgt
//
LOCUS       DASUMX010000049         3512 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_13631, whole genome shotgun sequence.
ACCESSION   DASUMX010000049 DASUMX010000000
VERSION     DASUMX010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3512
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13631"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..856
                     /locus_tag="VFZ86_02105"
     CDS             104..856
                     /locus_tag="VFZ86_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005562166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641113.1"
                     /translation="MTQGARPSQHGFALTRTAEHHARTLTAGSIAGMIFGVLFGVCSA
                     IVRYEVGESDVTGAWLRAGPSEVEWALGLLPFAGIFFLWFIGVSRQRLGRWEDRFIST
                     IILGSGALFLAMVFAAAALAGALIALYNRDPVGVPGSETYAFVQVAVSRIFGVYALRM
                     AAVFLICQATAWLRHGLMPRSLGLATYAVAFVLLFVVSEEAWAVLVFPAWVFVVSAYL
                     FAVSRREPEVAESAQAGPASGGAGETGEASPA"
     gene            complement(892..2325)
                     /gene="purF"
                     /locus_tag="VFZ86_02110"
     CDS             complement(892..2325)
                     /gene="purF"
                     /locus_tag="VFZ86_02110"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEX5641114.1"
                     /translation="MYDDRLHDSCGVFGIHAPGLDVARLTFFGLYALQHRGQESAGIA
                     VADDGQVTVIKDLGLVSQVFNEQMLQSLTGQHAIGHVRYSTTGSSHWSNSQPLVRSRN
                     GDVIALGHNGNLVNTTELRDELVAQGVRFSGTTDTEVITALIAHEAEGDLLEGVRTAV
                     SKIRGAFSAAVMSGDAVVGFRDPYGVRPLCLGDYEGHPVFSSETSGLDIIGADFVREI
                     QPGEIVIADSEHGLRSERVELEGARPALCIFEFIYFARPDSVMNGKTLHVVRQNMGRH
                     LSMEAPVEADLIVPVPDTGMPAAIGYAAASGIPYTEGLIKNRYVHRTFIQPDDNLRQV
                     GIRMKLNPLTSVIKGKRLVVVDDSIVRGNTTRRLVQMLFDAGAAEVHLRISSPPIQWP
                     CFYGIDMATRAELIAANKSVDEIRAHVGATSLHYLTLDGLQASTGLPRSQFCRACFDG
                     DYPIAVPEEYAMCKMRFETDATALCDP"
     gene            complement(2340..>3512)
                     /locus_tag="VFZ86_02115"
     CDS             complement(2340..>3512)
                     /locus_tag="VFZ86_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HEX5641115.1"
                     /translation="IRTPSVKPDYIVDEPVAVDAAAYPPAKDAGAALVQLMAAPNIAS
                     KRWAWEQYDYIVQANTVQIPGSDAAVLRIKGTDRGFAFSNDCNGRHCYLDPYRGAKAA
                     TAEAARNLACSGALPVAVTDCLNFGNPEKGEIYWQFEQAIEGMAEACEALDTPVVSGN
                     VSFYNESFGQAIYPTPMVGMLGIFDDVSVAIDAAFKDEGDVVLLLGDDAAWMDGSEYQ
                     KTAYGKVEGRIPDVDLELEVKLQARLRAAIEARLLKSAHDCAEGGLAVALAECCVFSG
                     SNGGAQTGEPEAVRPGRSGRWLGADVDFGSAEAITGAAGRPDLALFGEAPTRVVVTCA
                     PDKVAELEALLGDLPHRTVGRVTGADLRCTMDAKELFAVPVSELHSAYESLPERLA"
BASE COUNT          472 a         1143 c         1316 g          581 t
ORIGIN      
        1 tcgacgattg cgtgagcccg cgttcgccac ggcgtagact gtgcggggga cgtcaggacg
       61 ccgggcgtgt tcgggcgctc ggccacgagg ggaggcgagg atcgtgaccc agggggcgcg
      121 gcccagtcaa cacggcttcg cgctcacgcg cacggccgag caccacgcgc gcacgctgac
      181 cgcgggctcc attgcgggga tgatcttcgg tgtgctgttc ggcgtgtgct ccgccatcgt
      241 gcggtacgag gtcggcgagt cggacgtcac cggggcgtgg ctcagggctg gtcccagcga
      301 ggtggagtgg gcgcttggcc tgctgccgtt cgccggcatc ttcttcctgt ggttcatcgg
      361 cgtgtcccgg caacgcctag gacgctggga ggacaggttc atctcaacga tcatcctcgg
      421 cagcggcgcg ttgttcctcg ccatggtctt cgctgccgcg gctctcgcag gcgctctcat
      481 cgccctctac aacagggacc ctgtgggcgt ccctggcagc gagacctacg ccttcgtcca
      541 ggtcgcggtg tcgaggatct tcggcgtcta cgcgctgcgc atggcggcgg tgttcctcat
      601 ctgccaggcg acggcgtggc tgcggcatgg gctgatgccc agatcgctgg ggctggccac
      661 ctacgcggtc gcgttcgtgc tgctgttcgt ggtttctgag gaggcctggg ccgtgctcgt
      721 cttcccggcg tgggtgttcg tcgtcagcgc gtacctgttc gcggtgagca gacgggagcc
      781 ggaggtggcc gagagcgcgc aggccggtcc ggccagcggc ggcgcaggcg agaccggcga
      841 ggcgtcgccg gcctgacggc ggtcaggcgg agccggcgaa gggcgacacg ctcacgggtc
      901 gcagagcgcg gtggcgtccg tctcgaagcg catcttgcac atcgcgtact cctcgggcac
      961 ggcgatgggg tagtccccgt cgaagcaggc gcggcagaac tggctgcgcg gcaggccggt
     1021 gctcgcctgc aggccatcga gcgtgaggta gtgcaggctg gtggcgccga cgtgggcgcg
     1081 gatctcgtcc acgctcttgt tggcggcgat gagctcggcg cgggtggcca tgtcgatgcc
     1141 gtagaagcag ggccactgga tgggcggcga gctgatgcgc aggtggacct cggcggcgcc
     1201 ggcgtcgaac agcatctgca ccagccggcg ggtcgtattg ccgcggacga tggagtcgtc
     1261 cacgacgacg aggcgcttgc ccttgatcac cgaggtcagc gggttgagct tcatgcggat
     1321 gccgacctgg cgcaggttgt cgtcgggctg gatgaaggtg cggtgcacgt agcggttctt
     1381 gatgaggccc tcggtgtagg ggatgccgga ggcggcggcg tagccgatcg ccgccggcat
     1441 gcccgtgtcg gggacgggca cgatgaggtc ggcctccaca ggcgcttcca tcgagaggtg
     1501 ccggcccatg ttctggcgca cgacgtggag cgtcttgccg ttcatcacgc tgtccggccg
     1561 ggcgaagtag atgaactcga agatgcagag cgccgggcgc gcgccctcga gctcgacgcg
     1621 ctcgctgcgc aggccgtgct cgctgtcggc gatgacgatc tcgcccggct ggatctcgcg
     1681 cacgaagtcg gcgccgatga tgtcgagccc ggaggtctcg ctgctgaaca ccgggtggcc
     1741 ctcgtagtcg ccgaggcaga gcgggcgtac gccgtaagga tcgcggaagc cgaccacggc
     1801 gtcgccgctc atcaccgcgg cggagaaggc gccgcggatc ttgctgacgg cggtacgcac
     1861 cccctcgagc aggtcaccct cggcctcgtg cgcgatgagc gccgtgatga cctcggtgtc
     1921 ggtggtgccc gagaacctga ccccttgggc gacgagctcg tcgcgcagct ccgtggtgtt
     1981 cacgaggttg ccgttgtggc ccagggcgat gacgtcgccg ttgcgactgc gtaccagcgg
     2041 ctgtgagttc gaccagtggc tcgatcccgt cgtgctgtaa cgcacgtggc cgatggcgtg
     2101 ctggccggtg agactctgga gcatctgctc gttgaacacc tggctgacca ggcccaggtc
     2161 cttgatgacc gtgacctggc cgtcgtcggc cacggcgatg ccggcgctct cctggccgcg
     2221 atgctgcagg gcgtagagcc cgaagaaggt caggcgcgcg acgtcgagtc ccggcgcatg
     2281 gatgccgaag acgccacagc tgtcgtggag acgatcgtcg tacacaacgc tcggttcggt
     2341 caggcgagcc gctccgggag cgactcataa gcagaatgga gctcgctcac cgggacggcg
     2401 aagagctcct tcgcatccat ggtacacctg agatcggcgc cggtcacccg cccgaccgtg
     2461 cggtgcggca ggtcgccgag gagggcctcg agctcggcga ccttgtcggg cgcgcaggtc
     2521 acgaccaccc gcgtcggcgc ctcgccgaag agcgcgaggt ccggccggcc ggcggcgccg
     2581 gtgatggcct cggcggagcc gaagtcgacg tcggcgccga gccagcggcc ggagcggccg
     2641 ggccgcaccg cctcgggctc gcccgtctgg gcgccgccgt tggagccgct gaacacgcag
     2701 cactcggcca gcgccacggc gagcccgccc tcggcgcagt cgtgggcgct cttgagaagg
     2761 cgcgcctcga tggctgcgcg caggcgcgcc tggagcttca cctcgagctc caggtcgacg
     2821 tccgggatcc gcccctcgac cttcccgtag gcggtcttct ggtactcgga gccgtccatc
     2881 caggcggcgt cgtcgccgag cagcaggacg acgtcgccct cgtccttgaa ggcggcgtcg
     2941 atggcgacgc tcacgtcgtc gaagatgccg agcatgccga ccatcggcgt cgggtagatc
     3001 gcctggccga agctctcgtt gtagaagctc acgttgccgc tgacgacggg cgtgtccaga
     3061 gcctcgcagg cctcggccat gccctcgatg gcctgctcga actgccagta gatctcgccc
     3121 ttctccgggt tgccgaagtt gaggcagtcg gtgacggcca cgggcagcgc gccggagcac
     3181 gccaggttgc gcgccgcctc ggcggtcgcc gccttggcgc cgcggtacgg gtcgaggtag
     3241 cagtggcggc cgttgcagtc gttgctgaag gcgaagccgc ggtcggtgcc cttgatgcgc
     3301 agcaccgcgg cgtccgagcc ggggatctgc acggtgttgg cctggacgat gtagtcgtac
     3361 tgctcccagg cccagcgctt gctggcgatg ttgggcgcgg ccatgagctg caccagggcc
     3421 gcgccggcgt ccttcgccgg cgggtacgcg gcggcgtcca cggccaccgg ctcgtcgacg
     3481 atgtagtccg gcttgacgga gggcgtgcgg at
//
LOCUS       DASUMX010000050        10376 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1738929, whole genome shotgun sequence.
ACCESSION   DASUMX010000050 DASUMX010000000
VERSION     DASUMX010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 10376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10376
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1738929"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            182..514
                     /locus_tag="VFZ86_02120"
     CDS             182..514
                     /locus_tag="VFZ86_02120"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX5641116.1"
                     /translation="MEERLEREVNQLHASICQALADPKRILILYELSEGPKTVTELTE
                     ALKVPQPTVSRHLKMLREQRLVHSERDGVSIRYSLADVRVITALDTMRAVLLGGLAQQ
                     AELAEGLS"
     gene            516..1040
                     /locus_tag="VFZ86_02125"
     CDS             516..1040
                     /locus_tag="VFZ86_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009886307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEX5641117.1"
                     /translation="MTVESFEGRAPRLGAGCWVHPSADVIGDVTLGARCWIGPGARLR
                     GDYGTIVLGDCCAVEDNAVVHARPGETCAIGAWVTLGHGCVVHNVKLLGDYAVIGMNA
                     VVSDWAEVGEWAVVAEGAVGRQRAVVPAARIAAGVPARLLEREVDEAYRADWRRFKQI
                     YVDLCARYAEGYGR"
     gene            1096..1716
                     /locus_tag="VFZ86_02130"
     CDS             1096..1716
                     /locus_tag="VFZ86_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019062747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HEX5641118.1"
                     /translation="MSYVALLRAVNVGGTTLPMADLRALLEGLGLTDVRTYLQSGNAV
                     FDAGDGAEPAAALATAIETRIERDIGPRVGVLVLPGEAMLDVAAANPFLASEGGLASA
                     ENILHATFLFGAHGEADFGEASDQAYCAVYKAAFGKLELPAVDGEEAAFIGAPPLATP
                     VIYLKLPHGYGRTRLNNAWFERKLGTAATTRNWRTVLALAEMVGEA"
     gene            complement(1718..2224)
                     /locus_tag="VFZ86_02135"
     CDS             complement(1718..2224)
                     /locus_tag="VFZ86_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX5641119.1"
                     /translation="MADVQISAPPGVPQIVTAVEFDAPRYLVFRAFMEPDLLAQWLGP
                     RRVTMTIEEYDPRDGGRYRYTHHDPDGNTYSFRGVFHGEPSLEGAVQTFEYEGAPGHV
                     SLDTLTLEEQGGRTTVRTNSVFQAVEDRDAMVQAGMEKGVTEGMQRLEELLEKLKKQG
                     EGGASRAT"
     gene            2356..3255
                     /gene="zupT"
                     /locus_tag="VFZ86_02140"
     CDS             2356..3255
                     /gene="zupT"
                     /locus_tag="VFZ86_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HEX5641120.1"
                     /translation="MSGVWFALGLTLVAGMATGIGSAIAFTAKRTNYRFLSVATGFSA
                     GVMLYVSFVEIFFKGAAALTERYGEVTGYWVNTLAFFGGMAIIFVIDTLIPAGENPHE
                     THSEAETAPLHDPSAPVPLSSVTPGAADRGPGLHDHTADHHKLMRMGLFTALAIGIHN
                     FPEGLATFLAALEDPGLGIAIAIAIALHNIPEGISVSVPIFYATGDRKKAFTYSLLSG
                     LSEPVGAGIAYLVLILLLGGTGADVPQELMGILFAGVAGIMVYISLDELLPTSRAYGK
                     GHDSLYGLVAGMLVMALSLALMK"
     gene            3263..4204
                     /locus_tag="VFZ86_02145"
     CDS             3263..4204
                     /locus_tag="VFZ86_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HEX5641121.1"
                     /translation="MLLNAVFVIIGLLLLAAGAEGLVRGAGAVALRFGVAPLVVGLTI
                     VALGTGSPEFVLSVQAAASGSGGLALGNVLGSNISNIALVLGLAALVRPLAVQARLIR
                     REVPVMIGATFLLAVMMWDGGLGRLDGAVLLAGAVLYVAFAYYGARRSDVPAVAAEFD
                     AALERERSLRVEALLVVVGLGALLLGATVLLRGAVELASALGVSEVVVGLTVIAVGTS
                     LPELATSVSAARRGQADVAFGNAIGSNVINILAVLGAAALVRPLATSGLRPLDVAVFL
                     VSAVLVLPLMARGSVLNRWEGGALVAGYAVYIVSLAA"
     gene            4369..4920
                     /locus_tag="VFZ86_02150"
     CDS             4369..4920
                     /locus_tag="VFZ86_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641122.1"
                     /translation="MDDTPIMNATGKALGDWFAILDGRDARDLPHKDIARLLQDEYGV
                     SGWWSQNVTVEYEKHIGRRETGQTQAGDYEATVTRTLPGGMDAALDRWVAGLPADGAA
                     PGFDGVPFAGEPGISRTEKWRYWRVALADGTRVTVTISGKPGGAAKAGGPAALLAVTS
                     SKLPTRDDCDRWKAFWRRYLAAL"
     gene            4951..5412
                     /locus_tag="VFZ86_02155"
     CDS             4951..5412
                     /locus_tag="VFZ86_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003104636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX5641123.1"
                     /translation="MTVDVMTETLIRRPSDVVAEYAADPDNAPEWYANIESVEWKTPP
                     PLAVGSRISFVAQFLGRRLAYTYEVAEYVPGERLVMRTAQGPFPMETSYLWEATADSH
                     TRMTLRNRGTPAGFSSLAAPFMTASMRRANKKDLARLKTLLEARGQTAAAR"
     gene            complement(5454..5642)
                     /locus_tag="VFZ86_02160"
     CDS             complement(5454..5642)
                     /locus_tag="VFZ86_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006335471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641124.1"
                     /translation="MSDNSGAGAAGAGAVYGLGFIGALVYYIGGANSFWDGVWGVLQA
                     MVWPAFFVYGVMDKLNVP"
     gene            5778..6329
                     /locus_tag="VFZ86_02165"
     CDS             5778..6329
                     /locus_tag="VFZ86_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641125.1"
                     /translation="METFTEPRALVRDRNYAAERQAALEALDLSAVEPPIVDIVLAFR
                     TLPHCFTLQCCHGHFLTATGQDEHSLAPIPEGHEGAVRYRIAYLAFCVDAGDRGRAFL
                     ERLSRVPEIEPAYVQFGSADWFWDQWPNSYTLQVEPATHRYKDQAVLTAGEAMQTQRA
                     RDRFFEEIRRLLAEEVARYPFDG"
     gene            6418..7008
                     /locus_tag="VFZ86_02170"
     CDS             6418..7008
                     /locus_tag="VFZ86_02170"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5641126.1"
                     /translation="MPATRDEIALTFMGLALRYGFRRTAVEDVAKALRISKKTIYEYF
                     PSKEALLDHALELGAMEQRRRVESLLTEATALGRALQIVRIALSDARAGFAQNPGVEL
                     VEPPEIQTQVNDRVYGPMIRDLLEQGIAAGEFEVPDVDLTSRFAQAIGMEAVRRVHDD
                     PSSRPEEATVEAIRRLIAGGGQPVAKKIKKKKTTKR"
     gene            7019..7573
                     /locus_tag="VFZ86_02175"
     CDS             7019..7573
                     /locus_tag="VFZ86_02175"
                     /inference="COORDINATES: protein motif:HMM:NF024135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HEX5641127.1"
                     /translation="MMTQVLVAYGTRYGSTREVAETVAATLGEQGIETDVKRAREVRS
                     LDGCDAVVLGTPLYMGALHKDVRALLEKNRQALAELPFAVFALGPIKSADGLDGSREQ
                     LFTALAKVAAPTPMATGVFVGAYDPARLALRDRMIAALPASPLHGETAHDDRDWEAIQ
                     VWTRDLAGLLGLSAPGTGPSRPSA"
     gene            complement(7581..8561)
                     /locus_tag="VFZ86_02180"
     CDS             complement(7581..8561)
                     /locus_tag="VFZ86_02180"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX5641128.1"
                     /translation="MASPDDQQKLRGRRPGTVLVPQGALTRGAAFFIDTLLVSLVVGL
                     LLGDKAPLEMVVAATLVADFLYFALSEGITGTTLGKRLFGLRVVRAADGRSCGPLAAI
                     VRTALRLVDNLLFSLPGIAAIVTSPRRQRLGDRAARTLVVTEVPEQLLAAFDRISGRA
                     TPDEVLRRINAATLGHAQPDLTPGLAHVNHSPYPAAAAHGETLPCPFCDVPMPVDEVV
                     CRYCGEYVNQVTANGDAENMAPVPMLHSPDRRYRFDALWRLVFADDEASLAAVREAVP
                     AWPRADRLLAVNAFAEVADPRPAAFLESMTSDPDEAVCALAREVRGRRAP"
     gene            8708..9331
                     /locus_tag="VFZ86_02185"
     CDS             8708..9331
                     /locus_tag="VFZ86_02185"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5641129.1"
                     /translation="MKATTRSTKEDRRREIVEAATREFAVGGLHGTPVDAIAKRVGVS
                     QPYLFQLFGTKKELFIATVHRTFERTVGAFRTAAAEAGDDASQTDILMAMGVAYGNLL
                     DDRSLLQMQMQAYAACGDEEIRDVVRDEMLRLVSFVQSASGAPEAAIREWLAYGMLMN
                     VVAVMDLTRVDADWARIMCPVHGTEVVALAIDAVGKTERTGVRARGK"
     gene            9456..>10376
                     /locus_tag="VFZ86_02190"
     CDS             9456..>10376
                     /locus_tag="VFZ86_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009739666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEX5641130.1"
                     /translation="MSARAMTFWTFVVTSVAVFMVSLDNLVVTTALPVIKADLNASLS
                     GLEWTVNAYTLTFAVLLLTGAALGDRFGRKRMFLVGLVVFTAGSAAAALAPSIDALVA
                     ARALQGVGGAIVTPLTLTILSAAVPPARRGMALGIWGGVSGLAVALGPVVGGAIVEGL
                     SWQWIFWLNVPIGLVLLPVALTRLTESKGPNRSLDLPGLGLASAGLLGIVWGLVRGNE
                     HGWTSLGVAGPIIAGVVLLGAFVLWERRTPEPMVPLRFFRNRAFSAANVTSLLMSFGM
                     FGSIFLLAQFFQVVQGYSPLEAGLRTLPWTA"
BASE COUNT         1530 a         3555 c         3704 g         1587 t
ORIGIN      
        1 gccgccccgg agtcggggga gggcgtctgt gcgagcttgt gaatttgcat acttgcttat
       61 atacgcctat ccggatggac gagtcaaccg agacagaggt ggtccggttt gtcgggccgc
      121 gagggcggca ggcgccggcc ctgattgacg gcgcaggggc ccgcgccgga caatcggact
      181 catggaagag cgcctcgaac gcgaagtgaa ccagctgcac gccagcatct gccaggcgct
      241 ggccgacccc aagcgcatcc tcatcctgta cgagctgagc gaggggccga agacggtcac
      301 cgagctcacg gaggcgctca aggtgccgca gccgacggtg tcgcggcacc tcaagatgct
      361 gcgcgagcag cgcctcgtcc acagcgagcg cgacggggtg agcatccgtt actcgctcgc
      421 cgacgtccgc gtcatcacgg cgctcgacac gatgcgcgcc gtgctcctcg ggggcctcgc
      481 ccagcaagcg gagctggccg agggcctctc ctgacgtgac cgtcgagagc ttcgaaggca
      541 gggcgccgcg actcggcgcc ggctgctggg tccatccctc ggccgacgtc atcggcgacg
      601 tcacactggg cgcccgctgc tggatcggcc cgggcgcccg cctgcgcggc gactacggga
      661 ccatcgtcct cggcgactgt tgcgcggtcg aggacaacgc cgtcgttcac gcccgccccg
      721 gcgagacctg cgccatcggc gcctgggtga cgctcggcca cggctgcgtg gtccacaatg
      781 tgaaactcct cggcgactac gccgtcatcg gcatgaacgc cgtggtgagc gactgggccg
      841 aggtcggcga gtgggcggtc gtggctgagg gcgcggtggg gcggcagcgc gccgtggtgc
      901 cggcggcgag gatcgccgcc ggggtcccgg cgcgcctgct cgagcgcgag gtcgacgagg
      961 cctaccgcgc cgactggcgc cgcttcaagc agatctacgt ggacctctgc gcccgctacg
     1021 ctgagggcta cgggcgctga tccccggcgg acacgctgcg cgagcgtgtc accccatgca
     1081 gggtatgaag cgggtatgag ttacgtcgcc ctcctgagag ccgtgaacgt cggtggcacc
     1141 acactgccga tggcggacct gcgcgctctg ctcgaagggc tcggactgac ggacgtgcgc
     1201 acctacctgc agagcggcaa cgccgtcttc gacgccggcg acggcgccga gccggcggcc
     1261 gcgctcgcga ccgcgatcga gacacgcatc gagcgggaca tcgggcctcg ggtgggcgtc
     1321 ctcgtccttc ccggcgaggc gatgctcgac gtggctgcag ccaatccgtt cctcgcgagc
     1381 gaaggcggcc tggcttcggc tgagaacatc ctgcacgcca ccttcctctt cggagcgcac
     1441 ggcgaggccg acttcgggga ggcctccgac caggcgtact gcgccgtgta caaggccgcg
     1501 ttcggcaagc tggagctgcc ggcggtcgat ggagaggagg cggcgttcat cggcgcgccg
     1561 ccgttggcca ctccggtcat ctatctcaag ctgccgcacg ggtacgggcg caccaggctc
     1621 aacaacgcct ggttcgagcg caagctcggt actgccgcga ccacccgcaa ctggcggacg
     1681 gtgctggccc tcgcggagat ggttggcgag gcctgagtca cgtcgcccgg gacgctccgc
     1741 cctcgccctg cttcttcagc ttctcgagca gttcctcgag ccgctgcatc ccctcggtga
     1801 cgcccttctc catgccggcc tggaccatcg cgtcgcggtc ctccaccgcc tggaagaccg
     1861 agttggtgcg cacggtcgtg cggccgccct gctcttcgag ggtcagcgtg tccagcgaga
     1921 cgtgtccggg cgcgccctcg tactcgaagg tctggacggc gccctccaga ctgggctcac
     1981 cgtggaagac gccgcggaac gagtacgtgt tgccgtcggg gtcgtggtgg gtgtagcgat
     2041 accggccgcc gtcgcgcggg tcgtactcct cgatggtcat cgtgacccgg cgtggcccga
     2101 gccactgggc gagcaggtcg ggctccatga aggcacggaa cacgaggtac cgaggggcgt
     2161 cgaactcgac ggcggtgacg atctgcggca cgccgggtgg ggcggagatc tgcacgtcgg
     2221 ccatagtgga tcctcacctt ctctgtcgga gactgtccgg ggtcactcct ctggtaccca
     2281 cgcagtctcg cgatatgctc ggcggcgatg agtcggaacg gaggcgcgcg tgcgggatca
     2341 cggagctgag gcgggatgag cggcgtctgg ttcgcccttg gcctcaccct ggtggccggc
     2401 atggcgaccg gcatcggcag cgccatcgcc ttcaccgcca agcgcaccaa ctaccggttc
     2461 ctgtcggtag cgacggggtt ctctgccggc gtcatgctct acgtgtcctt cgtggagatc
     2521 ttcttcaagg gagccgctgc gctgacggag cggtacggag aagtgaccgg gtactgggtc
     2581 aacacgctcg ccttcttcgg cggcatggcg atcatcttcg tgatcgacac gctcatcccg
     2641 gcgggcgaga acccgcacga gacccactcc gaggccgaga cggcgccgct gcacgacccg
     2701 tcggcgccgg tgcccttgag tagcgtgacg cccggggctg cggaccgggg acccgggctc
     2761 cacgaccaca ccgcggacca ccacaagctc atgcgtatgg gtttgttcac cgcgctcgcg
     2821 atcggcatcc acaacttccc ggagggcctc gccaccttcc tcgcggcgct ggaggatccc
     2881 ggactcggca tcgccatcgc gatcgccatc gcgctgcaca acatccccga gggcatcagc
     2941 gtgtcggtcc cgatcttcta tgccaccggc gaccgcaaga aggcgttcac ctactctctg
     3001 ctcagcggac tttccgagcc ggtaggcgcc ggcatcgcct acctcgtcct catcctcctg
     3061 ctcggcggaa cgggcgccga cgtcccccag gagctcatgg gcatcctgtt cgccggcgtc
     3121 gccggcatca tggtgtacat cagcctcgac gagctgctcc ccaccagccg cgcatacggc
     3181 aaggggcacg acagcctgta cgggctggtc gccgggatgc tggtgatggc gctgagcctg
     3241 gcgttgatga agtaggacgc tgatgcttct caacgcggtg ttcgtcatca tcggcctcct
     3301 gctgctcgcc gccggcgccg agggtctcgt ccgcggcgcc ggcgccgtgg cgctgcgctt
     3361 cggcgtggcg ccgctcgtgg tcggtctcac catcgtcgct ctcggcacgg ggagccccga
     3421 gttcgtgctg agcgtgcagg cggcggcgag cggcagcggc ggcctcgcgc tcggcaacgt
     3481 gctcggctcc aacatcagca acatcgcgct ggtgctgggg ctggccgcgc tggtcaggcc
     3541 tctggccgtg caggcgcggc tgatccgtcg cgaggtcccg gtgatgatcg gcgcgacctt
     3601 cctgctcgcc gtgatgatgt gggacggcgg cctcggcagg ctcgacggcg ccgtcctgct
     3661 tgcgggcgcc gtgctctacg tcgccttcgc ctactacggc gcccgacgca gcgacgtgcc
     3721 ggccgtggcg gcggagttcg atgcagccct cgaacgcgag cgctcgctgc gggtggaagc
     3781 gctgctcgtc gtcgtggggc tgggggctct gctcctcggc gccaccgtgc tcctgcgcgg
     3841 cgccgtggag ctggcgtcgg ccctgggggt gagcgaggtc gtggtgggtc tcaccgtgat
     3901 cgccgtcggg accagcctcc cggagctcgc gacctcggtg tcggccgcgc gccgcggcca
     3961 ggcggatgtg gcgttcggca atgccatcgg ctcgaacgtc atcaacatcc tggccgtgct
     4021 cggcgcggcg gcgctggtgc ggccgctggc gacgagcgga ttgcggcccc tggacgtcgc
     4081 cgtgttcctg gtgagcgcag tgctcgtgct gccgctcatg gcgcgcggct cggtgctgaa
     4141 tcgatgggag ggcggcgcgc tcgtcgcggg ctacgccgtc tacatcgtct ctctggcggc
     4201 ctgacgccgg gcgcccgctg ggccggctca gcgacagacc gggatgcgct gatagcagcc
     4261 ctcgcgcgtg cggaacccga acccgagccc ctgtcccacg agaggtgcgc ggcccaggcg
     4321 agaccggacg cgcgcgtaga cttctcacgg acggggcagg cagggaccat ggacgacacc
     4381 ccgatcatga acgcgaccgg caaggccctg ggcgactggt tcgccattct cgacgggcgt
     4441 gatgcccgcg acctgccgca caaggacatc gcccggctgc tccaggacga gtacggcgtg
     4501 tccgggtggt ggagccagaa tgtgacggtg gagtacgaga agcacatcgg tcgccgcgag
     4561 accggtcaga cgcaggccgg cgactacgag gcgaccgtga ccaggacgct gccgggaggc
     4621 atggatgcgg cgctcgatcg ctgggtcgcg ggcctgcctg cagacggcgc ggcgccgggc
     4681 ttcgacggcg tccctttcgc gggtgagccc ggcatctcca ggacggagaa gtggcggtac
     4741 tggcgggtcg cgctggccga cggcacccgg gtgacggtga cgatctcagg caagccggga
     4801 ggagcggcca aggccggcgg cccggcggcg ctcctcgccg tcaccagcag caagcttccg
     4861 acgcgggacg actgcgaccg ctggaaggcg ttctggcggc ggtatctcgc cgcactctga
     4921 tggcggatca gcagacgagg gaggcccgag atgaccgtgg acgtgatgac ggagacgctc
     4981 atccgtcgcc ccagcgatgt ggtggcggag tacgccgccg accctgacaa cgcgcccgag
     5041 tggtacgcca acatcgagtc ggtggagtgg aagacgccgc cgccgctcgc ggtgggctcg
     5101 cggatctcct tcgtggcgca gttcctgggc cggcgcctcg cttacaccta tgaggtcgcg
     5161 gagtacgtgc ccggcgagcg cctcgtgatg cgcaccgctc agggtccctt ccccatggag
     5221 accagctacc tgtgggaggc gacggcagac agccacacca ggatgaccct gcgcaaccgt
     5281 ggcacgccgg ccggcttctc gtccctggcc gcaccgttca tgaccgcctc gatgcggcgc
     5341 gccaacaaga aggatctggc gcgcctgaag acgctgctgg aggcgcgagg tcagacggcg
     5401 gcggcgcgct gagactaccg gagcggcccg agcagaaggg ctccgatcgt gcgtcaggga
     5461 acgttcagct tgtccatcac gccgtagacg aagaacgccg gccagaccat cgcctggagc
     5521 acgccccaga ccccgtccca gaatgagttc gcgccgccga tgtagtagac gagcgcgccg
     5581 atgaacccga gcccgtacac cgctccggcc ccggctgctc ccgcgccact gttgtcggac
     5641 atgtcgcctt ccttccgcaa cggatgccgc tcgtcggcat cgcggtacgg tggagtctat
     5701 cgcggcgggc gcccgccggc gtgccgtcgc gcgcggcaac gacgcgggtc gcgccgggtg
     5761 ggatactctg agaagcgatg gagaccttca ccgaacccag agcgctcgtg cgcgacagga
     5821 actacgccgc cgagcgacag gcggcgctcg aggccctcga tctcagcgcc gtcgagccgc
     5881 cgatcgtcga catcgtcctg gccttccgga cgctgcccca ttgcttcacg ctccagtgct
     5941 gtcacgggca cttcctcacc gcgacggggc aggacgagca cagcctggcg ccgatcccgg
     6001 agggtcacga gggagccgtg cgctaccgca tcgcctatct ggccttctgc gtcgacgccg
     6061 gcgaccgtgg ccgggccttc ctggagaggc tctcacgggt gccggagatc gagcccgcgt
     6121 acgtgcagtt cgggtccgcc gactggttct gggaccagtg gccgaactcg tacaccctcc
     6181 aggtggaacc cgcgacccac cgctacaagg accaggcggt cctcaccgcg ggcgaggcca
     6241 tgcagacgca gcgcgcccgg gaccggttct tcgaggagat ccggcgactc ctggccgaag
     6301 aggtcgcccg ctatccgttc gacggctgaa gctgcggcac gtttgacgcg ccgtccctac
     6361 gggcgcatga tgtacgcaag aacggtaatc gtttcctagt acatcgagag caatcgcatg
     6421 ccggcgacca gggacgagat cgcgctgacc ttcatgggtc tggctctgcg ctacggcttc
     6481 cgccgcaccg ccgtggagga cgtcgccaag gcgctgcgca tatcgaagaa gaccatctac
     6541 gagtacttcc ccagcaagga ggcgctgctc gatcacgcgc tcgagctcgg ggcgatggag
     6601 cagcgcagac gggtggagtc gctgctcacg gaagcgactg cactgggccg cgcgctgcag
     6661 atcgttcgta tcgcgctctc cgacgcgcgc gccggcttcg cccagaaccc cggcgtcgag
     6721 ctcgtcgagc cgccggagat ccagacccag gtcaacgacc gggtgtacgg ccccatgatc
     6781 cgcgacctgc tggagcaggg gatcgcggcg ggggagttcg aggtccccga cgtcgatctg
     6841 acgagccgct tcgcgcaggc gatcggcatg gaggcggtac ggcgggtcca cgacgacccg
     6901 tcgagccgac cggaggaggc gacagtcgag gcgatccggc ggctgatcgc cggcggggga
     6961 cagccggtcg cgaagaagat caagaagaag aagacgacga aaaggtgatg gggtgaagat
     7021 gatgacgcag gtcctggtcg catacggaac gcgctacggt tccacgcgcg aggtcgccga
     7081 gacggtggcg gcgacgctcg gggagcaggg catcgagacg gatgtgaagc gggcgaggga
     7141 ggtgcgctcg ctggacggct gcgacgccgt cgtgctcggt acaccgctct acatgggcgc
     7201 gttgcacaag gacgtccgcg ccctgctcga gaagaaccgg caggccctcg cggagctgcc
     7261 gttcgcggtc ttcgcgctcg gcccgatcaa gtccgccgac ggtctggacg gctcccgcga
     7321 gcagctcttc accgcgctcg ccaaggtggc ggcgccgacg ccgatggcca caggggtgtt
     7381 cgtcggcgcc tacgacccgg cgcgactcgc cctcagggac cggatgatcg ccgctctccc
     7441 ggcgagtcca ctgcacggcg agaccgccca cgacgaccgc gactgggagg ccatccaggt
     7501 gtggacgcgg gatcttgccg gcctcctcgg cctcagcgcg cccgggaccg gtccctcgcg
     7561 gccgtccgcg tagccgccgc ctacggcgcg cgccgcccgc gcacctcgcg cgccagcgcg
     7621 cacacggcct cgtccgggtc ggacgtcatg gactcgagga aggccgcggg acgcgggtcg
     7681 gccacctctg cgaaggcatt cacggccagc agccggtccg cccgcggcca ggccggtaca
     7741 gcctcgcgta ccgccgccag cgatgcctcg tcgtcggcga agaccagccg ccacagagcg
     7801 tcgaaccggt agcgccggtc gggggagtgg agcatcggca cgggggccat gttctccgcg
     7861 tcgccgttcg ccgtcacctg gttcacgtac tcaccgcagt agcggcagac gacctcgtcc
     7921 acgggcatgg gcacgtcgca gaaggggcac ggcagcgtct ctccgtgagc tgccgccgcc
     7981 gggtacggtg aatggttcac gtgcgccagc cccggggtga ggtcgggctg tgcgtggccc
     8041 agcgtcgccg cgttgatccg ccgcaggacc tcatccgggg tcgcgcgccc agagatccgg
     8101 tcgaacgccg cgaggagttg ctcggggacc tccgtcacga cgagcgtcct cgccgcgcgg
     8161 tcgcccagcc gctggcggcg cggggaggtc acgatcgcgg cgatccccgg caaggagaac
     8221 agcaggttgt ccacgagacg gagggccgtc cgcacgatgg ccgccagcgg gccgcaggag
     8281 cggccgtccg cggcccgcac cacgcgcagg ccgaagaggc gcttgccgag cgtggttccg
     8341 gtgatgccct cggagagggc gaagtagagg aagtcggcga cgagggtggc cgcgaccacc
     8401 atctccagcg gcgccttgtc tcccaagagg aggcccacga ccaggctgac cagcaacgtg
     8461 tcgatgaaga aggcggcgcc ccgcgtcagc gccccctggg ggaccagcac ggtcccgggg
     8521 cgtctgccgc gtagcttctg ctgatcgtcg gggctcgcca tcctcaccat cctcgccacc
     8581 acgcattcga gtatgcgggc ggtgcagcgc gcccgccacc cgggtcgccg tcatcgacgg
     8641 aggtcggact tgacaggtgc cgtcgaatca tgtttagtga ctactaagta agggaagcag
     8701 ggacaccatg aaggcaacga cgcgcagcac caaggaagac cgccgacggg agatcgtcga
     8761 ggccgccacg cgtgagttcg ccgtcggcgg cctccacggc acccccgtcg acgccatcgc
     8821 gaagcgggtc ggggtgtcgc agccgtatct cttccagctc ttcggcacca agaaggagct
     8881 gttcatcgcg acggtgcacc ggacgttcga gcgcaccgtc ggcgcgtttc gcaccgccgc
     8941 ggccgaggcc ggcgacgacg cgagccagac cgacatcctg atggccatgg gcgtcgcgta
     9001 tgggaacctg ctcgacgacc gcagcctgct gcagatgcag atgcaggctt acgcggcgtg
     9061 cggggacgag gagatccgcg acgtcgtgcg cgacgagatg ctgcgcctgg tcagcttcgt
     9121 gcagtcggcc tcgggcgcgc cggaggcggc gatccgtgag tggcttgcct acggcatgtt
     9181 gatgaacgtg gtcgcggtca tggacctcac ccgggtcgac gcggactggg cgcgcatcat
     9241 gtgcccggtg cacggcaccg aggtcgtggc cctggccatc gacgcggtcg ggaagacgga
     9301 gcgcacgggg gtgagggcgc gcggcaagta gacgacgggc gggctgccgg cttctggccg
     9361 gacccgcccc ttttcaggcc tcgacaacta agtgattgct aagttacaag gtccggcggg
     9421 gcagggactc ccgggccgca aagggagatg aagagatgag tgcacgagca atgaccttct
     9481 ggaccttcgt ggtcacgagc gtcgccgtct tcatggtgtc gctcgacaac ctggtggtga
     9541 ccaccgcgtt gccggtgatc aaggctgact tgaacgcctc gctgtccggt ttggagtgga
     9601 cggtcaacgc gtacacgctg acgttcgccg tgctgctcct gaccggcgcc gcgctgggcg
     9661 accggttcgg gcgcaagcgc atgttcctgg tgggcctcgt ggtcttcacc gccggctcgg
     9721 cggcggctgc tctggcgccg agcatcgacg cgctggtcgc cgcgcgggcc ctgcagggcg
     9781 tcggcggcgc gatcgtcacg ccgctgaccc tgacgatcct ctccgcggcc gtacctccgg
     9841 ctcgccgcgg catggcgctc ggcatctggg gcggcgtcag cggcctggcg gtcgcgctcg
     9901 gcccggtcgt cggcggcgcg atcgtcgagg gcctctcctg gcagtggatc ttctggctca
     9961 acgtcccgat cggccttgtg ctgctgccgg tcgccctgac gcgcctgacc gagtccaagg
    10021 gtccgaaccg ttcactcgac ctgcccggcc tgggtctcgc cagcgcaggc ctgctcggga
    10081 tcgtctgggg tctggtgcgc ggcaacgagc acggctggac cagcctcggc gtggccggtc
    10141 ccatcatcgc cggcgtcgtc ctgctgggcg cgttcgtgct ctgggagcgt cgcacgccgg
    10201 agccgatggt gccgctgcgc ttcttccgca accgcgcctt ctcggcggcc aacgtcacct
    10261 ctctgctgat gagcttcggc atgttcggct cgatcttcct gctggcgcag ttcttccagg
    10321 tggtgcaggg gtactcgccg ctcgaggcgg ggctgcgcac cctgccgtgg acggcg
//
LOCUS       DASUMX010000051         6137 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1163713, whole genome shotgun sequence.
ACCESSION   DASUMX010000051 DASUMX010000000
VERSION     DASUMX010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 6137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6137
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1163713"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="VFZ86_02195"
     CDS             complement(<1..405)
                     /locus_tag="VFZ86_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013979865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641131.1"
                     /translation="MKPWKLPPRAKVFEAFTAVADGRVRLAGPGAATVASSRGDKTYD
                     VGWSDDGRVVTSNDNASYWQGYLGYPVLAVLLARGVLHADAAAVDAMAGVRWHDLNTR
                     FKRDYEAAVAHVLGELSAHGGDPALVEREVAAV"
     gene            634..1842
                     /locus_tag="VFZ86_02200"
     CDS             634..1842
                     /locus_tag="VFZ86_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020888022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase family protein"
                     /protein_id="HEX5641132.1"
                     /translation="MTDEARRQDGEDRELSPATLAVRAGEDAARAHRGTQVPIVYSAA
                     FGYEDVDTWVDAALGRREGHIYSRNTNPTVAAFEEKVRLLEGAEAATSFSTGMAAVSN
                     TLFALVAPGERVVSVRDTYGGTNQMFADFLPRIGVEVELCDTSDFDQIEGAIAKGCRL
                     VYLESPTNPTLKVMDIARLSKAAHEAGAIVVADNTFATPINQNPLALGTDLVLHSATK
                     FLGGHADALGGVLCGPPDLIKKVYHYREITGASLDPMAAYLLVRSLKTLALRVRQQNE
                     NAMIVARWLAGRPEVEAVNYPGLETHLHHDVAARQMRGYGGMLSFSLRGGFDAVKRVL
                     PRLRLAHLAANLGAVETLAGVPATTSHVECTADERAALGIPEGLVRYSVGIEDAGDLI
                     ADLEQAFTVA"
     gene            1911..3350
                     /locus_tag="VFZ86_02205"
     CDS             1911..3350
                     /locus_tag="VFZ86_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641133.1"
                     /translation="MADKQHENGETGSPEPGEVTEVTQASAPAEAEPSAAPAAKTQSA
                     VSDEDDEVTAVAAAPARRPSRLRRLLVGLLIFLSCLAVVVTGLTLWTHYTVMNTDGYM
                     NLVGPIGKDPEAIQNLSEYVGGQVVAATDLQQRVTDALPDRVQLLAGPITSYVEDFIT
                     KGANKVLSSPRAYELWLEVNRRGHEKIVALLRGETTNAYIAGSDVKLNLLPLVSQVLL
                     WVDERLPGGLSDRLNPPVIEPGTDPEAGIQEVANWSGKPLPSDFGQITLLQADALGPA
                     QTAVKWFDRLTWVLPLVTVGLIALTIWLSRRRARTAIAIGIGAAIAIFVTRVIVKRAS
                     LYLTDKLQEGQGDTIVRDVVNASLGPLTTITIWICVIGVVAALLVWLLGRRDVRAGVV
                     AAGRRAVGQAAEISIPDSPVTSWIVNHVVGVRWGVLVVGLIVLALVASSWLGIILSIV
                     VVLLLEGFLSFMTGQWPFNEREGDESTAT"
     gene            3387..4769
                     /locus_tag="VFZ86_02210"
     CDS             3387..4769
                     /locus_tag="VFZ86_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012508602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEX5641134.1"
                     /translation="MTGHDDTRAEGAAGGAGWAQPDPAPTTVAVDLALEPPADLEEDS
                     IPRGAADFVRDLFSRELLRYLGPGFIVTIGFIDPGNWATNIAGGSEFGYTLLWVVSLS
                     TLMLIFLQHLSAKLGIVTGHSLAANIRTKLPRPVVWLAGVTIVLACMATDLAEYLGAA
                     LGFYLLFGLPLWIGAPLTVGIVFIAILGQQYHRLERMIVVFLAIIAGCYIVELFLVKP
                     DWAAAAPRWVIPSVDGASILVALGMLGAIVMPHNIYVHSNVILSRDWDLAPDRRRRLM
                     NFELIDTSMAMGVGWLVNSAMIIVAAAVFFTAGITVTSIEQASETLEPLVGSLSQFLF
                     GMALLVAGIASSITSSLAEANVVTGYLGKPEDPRTRAYRIGLVVTSLPAMLVIALGVD
                     SFKALIVSQVLLSIQLPFTIIPLLWLSRSRRVMGADRMNMIQLGVGVLLAAIIIGLNA
                     FLLYQIFFGG"
     gene            4773..5186
                     /locus_tag="VFZ86_02215"
     CDS             4773..5186
                     /locus_tag="VFZ86_02215"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5641135.1"
                     /translation="MGIYDHILVPLEGGEIDAAVLEHVGRLAEACGADVTLLRVAHFH
                     TRDQRACELEDAEGALARAAEQLRRRGLTVQTRLLSGEPQQVIVEQAEELHPDLIAMA
                     THGHGWAKRMVLGSVADHVRHNSDVPLLLLKGGGA"
     gene            5243..5944
                     /locus_tag="VFZ86_02220"
     CDS             5243..5944
                     /locus_tag="VFZ86_02220"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEX5641136.1"
                     /translation="MPDRLILFDIDCTLVDARGAGGRAILRAIKDVYGVEGELGDYSF
                     HGRTDPGIIGDLASLWGACEPGAAAGADGLVDACIARYVELLREEIARVEVETLPGIR
                     ELVTALVSDRRVLVGLLTGNVEEGARLKLEPTGLLPLFKVGAYGSDSGLRADLPAVAV
                     ARAEARDGRRYAGKDIVVIGDSPADIECGASLGVTAVGVATGRHTVGELARYEPDHLF
                     TDFTDWRAAYAAIIG"
BASE COUNT          880 a         2226 c         2097 g          934 t
ORIGIN      
        1 cacggcggcg acctcgcgct ccacgagcgc cgggtcgccg ccgtgcgccg acagctcgcc
       61 gagcacgtgc gcgacggccg cctcgtagtc gcgcttgaac cgcgtgttga ggtcgtgcca
      121 gcggacgccg gccatggcgt cgaccgcggc cgcgtcggca tggagcaccc cacgggcgag
      181 cagtacggcg aggaccgggt agccgaggta cccctgccag tacgaggcgt tgtcgttact
      241 cgtgaccacg cggccgtcgt ctgaccagcc gacgtcgtag gtcttgtcgc cgcgcgagga
      301 ggcgacggtc gcggcgccgg gcccggcaag ccggacccgg ccgtcggcga ctgccgtgaa
      361 cgcctcgaag accttggctc tgggcggcag cttccagggc ttcatgcccg cctcctcgtc
      421 ctgctcgtgt gctgccgggc gtcgccccaa ctgtacccgg cccgccccgg tcgcgagccc
      481 ccgcggcggc gacgcggact ccggcgggcc gggaacggag tcactgcaga cctccgcgga
      541 ccccgccggg ccgagagggc ctagaatgac gcggacgatc acaggaggtc cggcgccttg
      601 cgctccgggc ggcgagcacg gcgggagggt gtcatgaccg acgaggcgag acggcaggac
      661 ggcgaggaca gagagctctc gcccgccacg ctggcggtgc gggcgggcga ggacgcggcg
      721 cgggcccatc gcggcacgca ggtgccgatc gtgtacagcg ccgcgttcgg ctacgaggac
      781 gtggacacgt gggtcgacgc cgcccttggg cggcgtgaag gccacatcta ctcgcgcaac
      841 accaacccga cggtggcggc gttcgaggag aaggtccggc tgctcgaggg cgcggaggcg
      901 gccaccagct tctccaccgg catggccgcc gtcagcaaca cgctcttcgc gctggtcgcc
      961 ccgggcgagc gcgtcgtctc cgtcagggac acgtacggcg gcaccaacca gatgttcgcg
     1021 gacttcctgc cgcgcatcgg tgtcgaggtg gagctctgcg acaccagcga cttcgaccag
     1081 atcgaggggg ccatcgccaa gggctgccgc ctcgtctacc tcgagtcgcc gaccaacccg
     1141 acactgaagg tgatggacat cgcccgcctc tcgaaggccg cgcacgaggc gggcgccatc
     1201 gtcgtcgccg acaacacgtt cgcgacgccc atcaaccaga acccgctggc gctgggcacg
     1261 gacctcgtgc tgcacagcgc gaccaagttc ctcgggggcc atgccgacgc gctcggcggc
     1321 gtgctctgcg gtccccccga tctcatcaag aaggtctacc actaccgcga gatcaccggc
     1381 gcctcgctcg accccatggc cgcctacctg ctcgtgcgca gcctcaagac gctggcgctg
     1441 cgcgtgcgcc agcagaacga gaacgccatg atcgtcgcgc gctggctggc ggggcggccg
     1501 gaggtcgagg cggtcaacta ccccggcctg gagacccacc tgcaccacga cgtggcggca
     1561 cggcagatgc gcggctacgg cggcatgctg agcttctcgc tccgcggcgg cttcgacgcg
     1621 gtcaagcgcg tgctgccacg cctgcggctc gcgcacctcg ctgccaacct cggcgccgtt
     1681 gagacgctgg ccggcgtgcc ggcgaccacg agccatgtcg agtgcacggc cgatgagcgc
     1741 gccgcactgg gcattcccga ggggctcgtc cgctactcgg tgggcatcga ggacgccggc
     1801 gacctgatcg ccgacctcga acaggcgttc acggtcgcgt aggctttccg cctgcgacgg
     1861 ggaatgtgtg accattcggt aagcagcagt gatgcgaggg gggaccggcc gtggccgaca
     1921 agcagcacga gaacggtgag accggctctc ccgagccggg agaggtcacc gaggtgaccc
     1981 aggcctcggc cccggccgag gccgaaccat ccgcggcgcc ggcggcgaag acgcagagcg
     2041 ccgtcagcga cgaggacgac gaggtcaccg cggtcgcggc cgcgccggcc cggcgcccga
     2101 gccgcctacg ccggcttctc gtcggcttgc tgatcttcct ctcctgtctg gccgtggtgg
     2161 tcaccgggct cacgctctgg acccactaca cggtcatgaa caccgacggc tacatgaacc
     2221 tcgtcggtcc catcggcaag gacccggagg ccatccagaa cctcagcgag tacgtcggcg
     2281 gacaggtggt ggccgcgacc gacctgcagc agcgggtgac ggacgccctg cccgatcgcg
     2341 tccagttgct cgcggggccg atcacgagct acgtcgagga cttcatcacc aagggagcca
     2401 acaaggtcct cagctctccc agggcctacg agctctggct cgaggtcaac cgccgcggcc
     2461 acgagaagat cgtggccctg ctgcgcggcg agacgaccaa cgcctacatc gcgggcagcg
     2521 acgtgaagct gaacctgctg ccgctggtca gccaggtgct cctgtgggtc gacgagcggt
     2581 tgcccggcgg gctctccgac cggctcaacc cgcccgtcat cgagcccggc accgaccccg
     2641 aggccggcat ccaggaggtg gccaactggt cgggcaagcc gctgccctcc gacttcggcc
     2701 agatcacgct gctccaggcc gacgcgctcg gaccggcgca gacggccgtc aagtggttcg
     2761 accgtctcac ctgggtcctg ccgctggtga ccgtcggcct catcgccctg accatctggc
     2821 tgtcgcgccg ccgggcgcgg acggcgatcg ccatcggcat cggcgccgcc atcgccatct
     2881 tcgtcacgcg ggtgatcgtc aagcgcgcct cgctctacct gaccgacaag ctgcaggagg
     2941 gccagggaga caccatcgtc agggacgtcg tgaacgcgtc gctcgggccg ctcaccacga
     3001 tcaccatctg gatctgcgtg atcggcgtcg tcgcggccct gctcgtgtgg ctgctgggcc
     3061 gccgtgacgt gcgcgccggc gtggtcgcgg ccggcaggcg cgccgtcggc caggctgccg
     3121 agatcagcat ccctgactct cccgtcacct cctggatcgt caaccacgtc gtgggcgtgc
     3181 gttggggcgt cctcgtcgtc ggtctgatcg tcctcgcgct ggtggcgtct tcctggctcg
     3241 gcatcatcct gagtatcgtg gtcgtcctgc tgctggaggg cttcctcagc ttcatgaccg
     3301 ggcagtggcc gttcaacgag cgggagggcg acgagtccac ggccacctga ggagcccggc
     3361 cccgcaccac gggatagagt gtcgtcatga ccgggcacga cgacacgcgc gcggaagggg
     3421 ccgcgggagg ggcgggctgg gcgcagcctg atcccgcccc cacgacggtc gcggtcgacc
     3481 tggcgctcga gccgccggcc gacctcgagg aggattccat cccgagaggg gccgcggact
     3541 tcgtccgcga cctgttctcg cgcgagctgc tgcgctatct cggccccggg ttcatcgtca
     3601 cgatcggctt catcgatccg ggcaactggg ccaccaacat cgccggcggc tccgagttcg
     3661 gctacacgct gctctgggtg gtctcgctga gcacgctcat gctcatcttc ctgcagcacc
     3721 tctccgccaa gctcggcatc gtcaccggcc actcgctggc ggccaacatc cgcaccaagc
     3781 tgcccaggcc ggtcgtgtgg ctcgccgggg tgaccatcgt ccttgcgtgc atggcgacgg
     3841 acctggcgga gtacctcggc gccgcgctcg gcttctacct gctcttcggc ctgccgctgt
     3901 ggatcggcgc cccgctcacc gtcggcatcg tcttcatcgc gatcctcggg cagcagtacc
     3961 accggctgga gcggatgatc gtcgtcttcc tcgccatcat cgccggctgc tacatcgtcg
     4021 agctctttct cgtgaagccc gactgggcgg cggcggcgcc gcgatgggtg atcccctccg
     4081 tggacggcgc cagcatcctc gtcgcgcttg gcatgctcgg agccatcgtc atgccgcaca
     4141 acatctacgt gcactccaac gtcatcctgt cccgcgactg ggatctcgcc cccgaccgcc
     4201 gccgccgcct gatgaacttc gagctcatcg ataccagcat ggcgatgggc gtgggctggc
     4261 tcgtcaactc cgcgatgatc atcgtcgccg ccgccgtgtt cttcaccgcc ggcatcaccg
     4321 tcaccagcat cgagcaggcg tcagagacgc tcgagccgct ggtcgggtcg ctgtcgcagt
     4381 tcctgtttgg gatggcgctt ctggtggcgg ggatcgcttc gtcgatcacc tcctcgctcg
     4441 ccgaggctaa cgtcgtcacc ggctatctcg gcaagcccga ggatccgcgc actcgcgcat
     4501 acaggatcgg ccttgtcgtc acatcgctcc ccgcgatgct ggtcatcgcc ctgggggtcg
     4561 actccttcaa agccctgatc gtgagccagg tgctactgag catccagctg ccgttcacga
     4621 tcatccccct gctctggctc tcgcgcagcc gccgcgtcat gggcgccgac cgcatgaaca
     4681 tgatccagct cggggtcggc gtgctgctgg ccgcgatcat catcggactg aacgcgttcc
     4741 tcctctacca gatcttcttc ggaggctgac gaatgggcat ctacgaccac atcctggtgc
     4801 cgctcgaggg cggcgagatc gatgctgccg tgttggagca cgtcggcagg ctcgccgagg
     4861 cctgcggcgc cgacgtcacg ctcctgcgcg tggcccactt ccacacgcgc gaccagcgtg
     4921 cctgcgagct cgaggatgcg gagggtgcac tcgcgcgggc cgcggagcag ctccgccgcc
     4981 gcggcctcac cgtccagacc cggctgctct ccggggagcc gcagcaggtc atcgtggagc
     5041 aggccgagga gctccacccg gacctgatcg ccatggccac gcacgggcac ggatgggcga
     5101 agcgcatggt gctgggcagc gtggccgacc acgtgcgcca caacagcgac gtgccgctcc
     5161 tgctgctcaa gggcggcggg gcctgaggcg cccgccggcg ggtccgaatg cgctccggcc
     5221 cgcccgctat acttgcccgg cgatgcctga ccgcctgatc ctcttcgaca tcgactgcac
     5281 gctggtcgac gcccgcggcg ccggcgggcg cgccatcctg cgcgcgatca aggacgtcta
     5341 cggggtcgag ggcgagctcg gcgactactc gttccacggg cgcaccgacc ccggcatcat
     5401 cggcgacctc gcgtctctgt ggggcgcgtg cgagccgggc gcggcggccg gcgccgacgg
     5461 gcttgtcgac gcctgcatcg cacgctacgt cgagctgctg cgcgaggaga tcgcgcgcgt
     5521 ggaggtggag acgctgcccg gcatccgcga gctggtcacc gcgctcgtgt ccgaccgtcg
     5581 cgtcctcgtc ggcctgctca ccggcaacgt ggaggagggg gcccgtctca agctcgagcc
     5641 gacgggcctg ctccctctct tcaaggtcgg cgcctacggc tcggactcgg ggctgcgggc
     5701 cgacctgccg gccgtggccg tcgcccgcgc cgaggcgcgc gacggccggc gctacgccgg
     5761 caaggacatc gtggtcatcg gcgacagccc ggcagacatc gagtgcggcg cgtcgctcgg
     5821 ggtcacggcg gtcggcgtcg ccaccggccg gcacaccgtg ggcgagcttg cacggtacga
     5881 gccggaccac ctcttcaccg acttcacgga ctggcgcgcg gcctacgccg ccatcatcgg
     5941 ctgagcggcg cgcgcggcgc tctcatcacc gctcgcgccc ggctcacttc gcattaacgc
     6001 ccctcgcaca cttggcggcg atgctttcgc cgacagccgt gcagggccca tcgcctgaag
     6061 cggactgcgg cacctggagg cgttgagtgg ccagcgggcg gatcagagga tcttacgact
     6121 gggagtggcg agaagag
//
LOCUS       DASUMX010000052         2738 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_589604, whole genome shotgun sequence.
ACCESSION   DASUMX010000052 DASUMX010000000
VERSION     DASUMX010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_589604"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="VFZ86_02225"
     CDS             <1..227
                     /locus_tag="VFZ86_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641137.1"
                     /translation="GVLLVKAERHSRAEDAAELQAHRDSVWAEVSHLLDDMVEEPDAG
                     APEPDAAKPAPAAGLPAPAAGADRVPPPLL"
     gene            complement(341..1720)
                     /locus_tag="VFZ86_02230"
     CDS             complement(341..1720)
                     /locus_tag="VFZ86_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009396851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX5641138.1"
                     /translation="MAECVPNRKRIAELMEKEEKRLENETPKSFELYQRAIKHMPMGV
                     ASSYQARDPYPVYFTHGKGPHIWSVEGREIVDFHNGFGCMVQGHAHPVIVEAIQKRAP
                     LGTQFALPTEDSIIMAENLAGAFKLPKWRFVNSGSEATMDAIRVARAFTGREPIVKMF
                     GSYHGHHDYVMVSIGALDYGKIGPRDDYASLSYGAGIPQSSIDLTVAVPFNDIENITR
                     RIERMVEEGNPPACVIMEAAMMNLGVVLPEPGFLEGIRELTRKHGIVLIFDDVKVGIC
                     TARGGSVERFGVVPDMVTLGKALAGGTPAGAIGGSDEVMAMVNDGTVFQVGTYSGNPL
                     AMAAARASFEQVMTDDAYKHLNHLNDRIIAGCDAVCSKYDFPGYTVGISSKGCVNFAL
                     DKITDYESFIKTQDHELVALAWLYNINRGIIIAPGREEEWTLSVTHTDADIDLYVSAF
                     EDLVRDVTA"
     gene            complement(2213..>2738)
                     /locus_tag="VFZ86_02235"
     CDS             complement(2213..>2738)
                     /locus_tag="VFZ86_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641139.1"
                     /translation="VDNGYADAGAARDALVEQPSNTWTTTVRAFLSSFTNVEFVESEG
                     SFGLHNWDYSREIVNKAMMQAKIAESGVLVKLPYKVTFKISKSPVNAGAKVTFSGTVK
                     TAKGVAAAGTVKIMKRVGGVWKVWQRTTLKSDGSYKLTKKMTTKGNFYMRGLMPADSL
                     NLTGQSKQVRLIVR"
BASE COUNT          411 a          862 c          983 g          482 t
ORIGIN      
        1 cgggcgtgct gctggtcaag gcggagcgcc actcgcgcgc ggaggacgcc gcggagcttc
       61 aggcgcaccg cgacagcgtg tgggcggagg tcagccacct tctcgacgac atggtcgagg
      121 agccggatgc cggagcgccc gaaccggacg ccgcaaagcc cgcacctgcc gccggactgc
      181 ccgcacccgc cgccggagcg gaccgggtgc cgccgccgct gctctgacgg gtgcggcggg
      241 ggcagggacg acgaaagggg cgggcggccc gcaggccgcc cgcccctttt tcacgtctga
      301 gagggccggc gccgctcccg gcggccggcc gcacctctgc tcaggcggtg acatcgcgga
      361 cgaggtcctc gaaggcgctg acgtagagat cgatgtcggc gtcggtgtgg gtgacggaga
      421 gcgtccactc ctcttcgcgg cccggggcga tgatgatgcc gcggttgatg ttgtacagcc
      481 acgccagcgc gacgagctcg tggtcctggg tcttgatgaa cgactcgtag tcggtgatct
      541 tgtcgagggc gaagttgacg cagcccttgg acgagatgcc gacggtgtag ccggggaagt
      601 cgtacttact gcagaccgcg tcgcacccgg cgatgatcct gtcgttcagg tggttgaggt
      661 gcttgtaggc gtcgtccgtc atgacctgct cgaagctggc gcgcgctgcc gccatggcga
      721 gcgggttgcc cgagtaggtg ccgacctgga agacggtgcc gtcgttgacc atcgccatga
      781 cctcgtcgct gccgccgatg gcgccggccg gcgtgccgcc ggcgagggcc ttgccgagcg
      841 tgaccatgtc gggcacgaca ccgaagcgct cgaccgagcc gccgcgggcg gtgcagatgc
      901 cgaccttgac gtcgtcgaag atcaggacga tgccgtgctt cctggtgagc tcgcggatgc
      961 cctcgaggaa gcccggctcg ggcagcacga cgccgaggtt catcatcgcg gcttccatga
     1021 tgacgcaggc cggcgggttg ccctcttcga ccatgcgctc gatgcgcctg gtgatgttct
     1081 cgatgtcgtt gaagggcacc gcgacggtga ggtcgatcga gctctgcggg atgccggcgc
     1141 cgtagctgag ggacgcgtag tcgtcgcgcg ggccgatctt gccgtagtcg agggcgccga
     1201 tactgaccat gacgtagtcg tggtggccgt ggtaggagcc gaacatcttg acgatcggtt
     1261 cgcggccggt gaacgcgcgc gccacgcgga tggcgtccat cgtggcctcg gagcccgagt
     1321 tgacgaagcg ccacttgggg agcttgaacg cgccggcgag gttctcggcc atgatgatgg
     1381 agtcttcggt cggcagggcg aactgcgtgc ccaacggggc gcgcttctgg atggcctcga
     1441 cgatgacggg atgcgcgtgg ccctgcacca tgcagccgaa gccgttgtgg aagtcgacga
     1501 tctcgcggcc ctcgacgctc cagatgtgcg ggcccttgcc gtgagtgaag tagaccgggt
     1561 acgggtcgcg agcctgatac gacgacgcga cgcccatcgg catgtgcttg atggcgcgct
     1621 gatagagctc gaacgacttg ggcgtctcgt tctcgagccg cttctcttcc ttctccatga
     1681 gttccgcgat gcgcttgcga ttcggtacgc actcggccat gtgggctgct ccttcacagt
     1741 cgtaccgcac cggcttgggc cggtgcggcg atacctgatg ctgacggcgc cgcgaaggtg
     1801 gggcggggcg gcgcgagacg gtgctcggtc agaagacggg cgtgggtcac ctcggtctgc
     1861 gtatcacccc acagtgcgtg tctcgtcccc cctgagacgc gggagccgcg ctcccgaagg
     1921 cccggtgcgg gccggatgac gccggggcga gcgggaacgc tcgcgtgcgt cagtgtacag
     1981 cttcatgcgc gggaagtgta gggttcggca acgaattgac cgtttcggca gccgaatcgg
     2041 caacatcagg acccgagcgc gtcggggggc ggcgtccgat tggcggcctg ggtcgcccgg
     2101 gcgagcgcac cagacggaag gcgccggtcc cggaaaggcg aagggcggcc ccctcgcggg
     2161 ggccgcccat cgcggcgccg gatccgagct gtgaggctcg ggtcagacgg tcttacctga
     2221 cgatcaatcg gacctgcttg ctctgccccg tgaggttcag ggagtcagcg ggcatcaggc
     2281 cgcgcatgta gaagttgccc ttggtcgtca tcttcttggt cagcttgtag gagccgtctg
     2341 acttgagggt cgtcctctgc cagaccttcc acacgcctcc cacgcgcttc atgatcttga
     2401 ccgtaccagc ggccgccaca cccttcgcgg tcttcacagt accgctgaag gtgaccttgg
     2461 cgcccgcgtt gaccggtgac ttcgagatct tgaacgtcac cttgtacggc agcttgacga
     2521 ggacacccga ctcggcgatc ttggcctgca tcatggcctt gttgacgatc tccctcgagt
     2581 agtcccagtt gtggagaccg aagctgccct cgctctccac gaactcgacg ttcgtgaagc
     2641 tgctgaggaa ggcgcgcacg gtcgtggtcc aggtgttcga aggctgttcg acgagagcat
     2701 cgcgggcagc ccccgcatcg gcgtagccgt tgtcgacg
//
LOCUS       DASUMX010000053         5759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1164914, whole genome shotgun sequence.
ACCESSION   DASUMX010000053 DASUMX010000000
VERSION     DASUMX010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 5759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5759
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1164914"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            50..679
                     /locus_tag="VFZ86_02240"
     CDS             50..679
                     /locus_tag="VFZ86_02240"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEX5641140.1"
                     /translation="MGDADDRLLGLFPLGIVLLPGEVVPLHIFEERYRKLIGERLEEG
                     EFGIVLADDDTVRECGTAARVVQLIEELDDGRMNILVQGERRFRIIEVREPADPETDY
                     LTAEVDYYRDSEPEASPKLRDAVLTVFAKMLSLMDVEVPEEPAGEGPLSFRIAAAVDF
                     GAPLKQELLESLSEEHRLETLLTVMTSLLPRLELRKEREEAIRGNGKGY"
     gene            complement(680..1654)
                     /locus_tag="VFZ86_02245"
     CDS             complement(680..1654)
                     /locus_tag="VFZ86_02245"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone reductase"
                     /protein_id="HEX5641141.1"
                     /translation="MKAVRIHEYGGPDVLVYEEVPTPEPGPAQILVKVAAATVNPVDV
                     SVREDRFPTPKKPPKILGSDGAGVVERTGEEVTSVEPGDRVSFSGLGIGSEGSYAEYA
                     LIAETQVVRLPDGLSFVDAAAIGMTFPAAYYGLVTRGALREGETVLVQGASGGVGSAS
                     VQLARALGARVLATASGPGAADLVLGLGAEAVIDFRTEDVPARVLELTEGKGVDLVHE
                     LVLSVNLPADVKMVAKGGRIVGTGQGPGPDATAPIGEAISKDVSVLFMNLNNAGRAGV
                     AAIATEIAAMAAAGRVKPVIGAELPLSEARRAHEMLAEDHLGKIVLLP"
     gene            complement(1793..2821)
                     /locus_tag="VFZ86_02250"
     CDS             complement(1793..2821)
                     /locus_tag="VFZ86_02250"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HEX5641142.1"
                     /translation="MTRRVKIMIAVPLAIVAVVALFATVGLVARSGSSDSSLSDEART
                     AGQMDGGAAEPAMGEEAVAASGSTLDGEDFAAAVPPASAPAAHYLLRTGDLSLLVARG
                     DLLPTVDRIKGMTTAMGGYVMSSTVGSHAGGPIEPLPLDDVAVSPEGEGSASVTFTDP
                     YASLVVRVPEELFETAIRRFSRLGDVQSVSTSSEDVTSQYVDVRARLRHFRAVERRLV
                     GFLQETTTVNQMLAVQDRIDQVQLTIEELTAQLKSLTESTSFSTLSVYLSEKDATAAI
                     HAGNTFGGTFWNSVELLGHGARVTALALTALGPFIAVFGAVAVVVWLAVRRLRKGRRQ
                     AAPPTLPA"
     gene            complement(2914..>5759)
                     /locus_tag="VFZ86_02255"
     CDS             complement(2914..>5759)
                     /locus_tag="VFZ86_02255"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HEX5641143.1"
                     /translation="VDVFPTLRSRPEIARHLREYFAREGVAAPAPLRWGISLTGQRSP
                     VAPLASAVIRSQMKGFAQRFIVGRDARGAIPALRALRRDGIGFTLDVLGEATVSEAEG
                     LAYQRTYLGLLDGLATEAASWPRVPVIDEAAWGPLPRVNLSLKITSLYSQIDPADFGG
                     SVVAVKDLLRPIFRKAVATGAALTLDLEQFRHRDLTLAVFTSLLDEGEFRDYRDAGVV
                     LQAYLRDADQDLTRLVTWARERGRPFNLRLVKGAYWDYETVIAAQEGWPVPVFTHKPD
                     TDAMYERLTREMLEAADVVRPAFASHNVRSLAVAVETARGLGLSRDAFELQMLHGMGD
                     PIKAAVGGMGLRLREYAPVGELIPGMAYLVRRLLENTANDSFLRQTFVEGAAVDQLVR
                     PPQTSADFGAEARRLPAIQPTDPRAPGPFRNRPHADFSRHENREAYDAALAAARSVLG
                     AHYPLRIGGRDIETARTLESVNPAAPDEVIGTVAYGGRAEAEAAVAAARAALPAWRDA
                     APAERASVLFRAAELLRAETMELSALMTLEAGKPRREANADVDEAVDFLEYYGREMLR
                     LGTPRRMGHVPGEHNLYFYQPRGVALVIAPWNFPLAILAGMTSAALVAGDPVIVKPAG
                     PTPVIAARLVRALEAAGAPPGTVNFLPSPGSEVGDFLVRHPGVDLIAFTGSMDVGLRI
                     ISQAARHPAEGGVKKVIAEMGGKNAIIVDADADLDVAVSETVASAFHYAGQKCSAASR
                     VIVLEAAHDEFVRRLVEAARSLVVGPAEDPATRVGPVISADAREKIEGYVARGMREAT
                     LAAVVEPPAGGWPGGGFYVGPHVFTDVRPDAAIAQEEIFGPVVAVLKATDLDEALAIA
                     DGVRYALTGGLISRSPATIARVRREFRVGNLYVNRGITGALVERQPFGGFRMSGIGSK
                     AGGPDYLLQFLEPRVITENTLRRGFAPSDDLAD"
BASE COUNT          777 a         2006 c         2201 g          775 t
ORIGIN      
        1 tgagggtcgt ccggccgcgc gcgcggcgag gcgaaccggg aggcgcgacg tgggtgacgc
       61 tgacgaccgg ctgctggggc tcttcccgct cggcatcgtg ctgctgccgg gcgaggtcgt
      121 cccgctgcac atcttcgagg aacgctaccg caagctcatc ggcgagcgcc tggaggaggg
      181 cgagttcggc atcgtgctcg ccgacgacga caccgtgcgt gagtgcggga cggcggcccg
      241 ggtcgtccag ctcatcgagg agctggacga cggccgcatg aacatcctcg tccagggtga
      301 gcggcggttc cgcatcatcg aggtgcgaga gcccgccgac cccgagaccg actacctgac
      361 cgccgaggtc gactactatc gggactctga gcccgaggcg tcgccgaagc tgcgcgacgc
      421 ggtcctcacg gtgttcgcga agatgttgtc cctcatggac gtggaggtcc cggaagagcc
      481 cgccggtgag gggccgctct cgttccggat cgccgccgcc gtcgacttcg gcgccccgct
      541 caagcaggag ctgctcgagt cgctcagtga ggagcaccgg ctggagacgc tgctgacggt
      601 gatgacctcg ctgctgccgc ggctcgagct gcgcaaggag cgcgaagagg cgatccgcgg
      661 caacggcaag gggtattagt cagggcagca gcacgatctt gccgaggtgg tcctcggcca
      721 gcatctcgtg cgcgcggcgc gcctcggaga gcggcagctc ggcgccgatc accggcttca
      781 ccctgcccgc cgccgccatg gccgcgatct ctgtggcgat ggcggcaact ccggcgcgtc
      841 ccgcgttgtt caggttcatg aagaggacgg acacgtcctt ggagatggcc tcgccgatgg
      901 gggcggtcgc gtcagggccc ggcccctggc cggtgccgac gatgcggccg cccttcgcga
      961 ccatcttcac gtcggctggc aggttcacgc tgaggacgag ctcgtggacg aggtcgacgc
     1021 ccttgccctc ggtgagctcg aggacgcggg cggggacgtc ctcggtgcgg aagtcgatca
     1081 ccgcctcggc gcccaggccg aggacgaggt cggcggcgcc cggcccgctc gccgtggcga
     1141 gtacgcgggc gccgagcgcc cgcgccagct gcacggaggc cgagccgacg ccccccgacg
     1201 cgccctgcac gaggaccgtc tcgccctcgc ggagcgctcc gcgcgtcacg aggccgtagt
     1261 acgccgcggg gaaggtcatg ccgatcgccg cggcgtcgac gaacgacagt ccgtccggga
     1321 ggcgcacgac ctgcgtctcg gcgatgagcg cgtactccgc gtagctgccc tcgctgccga
     1381 tgccgagacc gctgaacgag acgcggtcgc ccggctcgac cgaggtgacc tcttcgcctg
     1441 tccgctcgac gacgcccgcc ccgtccgacc cgaggatctt gggcggcttc ttgggcgtcg
     1501 ggaagcggtc ctcacgcacg gacacgtcca ccgggttcac ggtcgccgcc gcgaccttga
     1561 cgaggatctg tgcaggaccc ggctcgggag tcgggacctc ctcgtacacg aggacgtcgg
     1621 gcccgccgta ctcgtggatg cgcaccgcct tcaccgctgc ctcctcggcc gtggtcgtct
     1681 ggtctggcag gaccataccc ggcgcgcggc cggggctcac cggcgacgcg gtgagccccg
     1741 gccgggacgg gtctcggagc cggcgggggt gagggtcccc ggccgggcgg ggtcaggccg
     1801 gcagggtcgg cggcgccgcc tgccgcctgc ccttgcgcag ccggcggacg gccagccaga
     1861 cgaccacggc gacggcgccg aagacggcga tgaagggccc gagcgcggtc agcgcgagcg
     1921 ccgtgacgcg ggcgccgtgg ccgagcagct cgaccgagtt ccagaaggtg ccgccgaagg
     1981 tgttgccggc gtggatggcc gcggtcgcat ccttctccga cagatacacc gagagcgtgc
     2041 tgaaggaggt ggactccgtg agggacttga gctgcgcggt gagctcctcg atcgtcagct
     2101 gcacctggtc gatgcggtcc tgtaccgcca gcatctggtt gaccgtggtc gtctcctgca
     2161 ggaagccgac gagccggcgc tcgaccgcgc ggaagtggcg caggcgggcc cggacgtcca
     2221 cgtactgcga ggtgacatcc tcgctggagg tcgagacgct ctggacgtcg ccgagcctgg
     2281 agaagcgcct gatcgcggtc tcgaagagct cctcgggcac gcgcacgacg agcgaggcgt
     2341 aggggtcggt gaaggtgacg gaggcggagc cctcgccctc gggcgacacc gcgacgtcgt
     2401 cgaggggcag cggctcgatg gggccgcccg catggctgcc cacggtgctg gacatcacgt
     2461 agccgcccat ggcggtcgtc atgcccttga tccggtcgac ggtcggcagc aggtcgcccc
     2521 gggcgacgag cagggagagg tcgccggtgc gcagcaggta gtgggcggcg ggcgcggagg
     2581 cgggcggcac ggcggccgcg aagtcctcgc cgtccagcgt gctcccggag gcggcgacgg
     2641 cctcttctcc catcgccggc tcggccgcgc cgccgtccat ctggccggcg gtccgcgcct
     2701 cgtcggacag ggaggagtcc gagcttccgc tgcgcgcgac gagcccgacg gtcgcgaaca
     2761 gcgcgacgac ggcgacgatc gcgagcggga cggcgatcat gatcttgacc ctgcgagtca
     2821 tggccttctc ctccttggcc gcgcccccgc ggccggatgg gtcattctgg cggtgagacg
     2881 ccgggaggag gtgcggggtt caggcgcggg ccgtcagtcg gcgaggtcgt cggagggggc
     2941 gaagccgcgg cgcagggtgt tctcggtgat cacgcgcggc tccaggaact ggaggaggta
     3001 gtccgggccg ccggccttgg agccgatgcc gctcatcctg aagccgccga acggctggcg
     3061 ctcgacgagc gccccggtga tgccgcggtt gacgtacagg ttgccgacgc ggaactcacg
     3121 gcgcacccgc gcgatggtcg cggggctgcg gctgatgagc ccgcccgtga gcgcgtagcg
     3181 gaccccgtcg gcgatggcca gcgcttcgtc caggtccgtc gccttgagga cggcgaccac
     3241 ggggccgaag atctcctcct gggcgatggc agcgtcggga cgcacgtccg tgaacacgtg
     3301 cgggccgacg tagaagccgc cgccgggcca gccgcccgcg ggcggctcga cgacggcggc
     3361 gagcgtcgcc tcccgcatcc cgcgggcgac gtagccctcg atcttctcgc gcgcgtcggc
     3421 gctgatgacg gggccgacgc gcgtcgcggg gtcctcagcc gggccgacga cgaggctgcg
     3481 cgccgcctcc accagccggc ggacgaactc gtcgtgggcc gcctcgagca cgatgacccg
     3541 cgaggcggcc gagcacttct gcccggcgta gtggaaggcc gaggcgacgg tctcgctcac
     3601 ggccacgtcg aggtccgcgt cggcgtcgac gatgatcgcg ttcttgccgc ccatctcggc
     3661 gatcaccttc ttgacccccc cttcggcggg gtgccgggcg gcctggctga tgatgcgcag
     3721 gcccacgtcc atggagccgg tgaaggcgat gaggtccacg ccgggatgcc ggaccaggaa
     3781 gtcgccgacc tcactgcccg ggctgggcag gaagttgacc gtgccgggcg gcgcgccggc
     3841 ggcctcgagg gcgcggacca ggcgcgcggc gatcaccggg gtcggtcccg ccggcttcac
     3901 gatcaccggg tcgccggcga cgagggccgc gctggtcatg ccggcgagga tggcgagcgg
     3961 gaagttccag ggcgcgatca cgagggcgac gccgcgcggc tgatagaagt acaggttgtg
     4021 ctcgccgggg acgtggccca tgcgccgggg cgtgcccagg cgcagcatct cgcgcccgta
     4081 gtactcgaga aaatcgaccg cctcgtcgac gtcggcgttg gcctcgcggc gcggcttgcc
     4141 ggcctcgagc gtcatgagcg ccgagagctc catcgtctcg gcccggagca gttcggcggc
     4201 gcggaacagc acggaggcgc gctcggccgg cgcggcgtcc cgccacgccg gcagcgccgc
     4261 gcgcgccgcc gcgacggcgg cttcggcctc ggcgcgcccg ccgtacgcga ccgtgccgat
     4321 cacctcgtcc ggcgcggcgg ggttcaccga ctccagcgtg cgcgccgtct cgatgtcgcg
     4381 gcctccgatg cgcagcgggt agtgggcgcc cagaacgctg cgcgcggccg ccagcgccgc
     4441 gtcgtacgcc tcgcggttct cgtggcgcga gaagtcggcg tgcggacggt tgcggaaggg
     4501 accgggcgcc ctcgggtcgg tcggctggat cgcgggcagc cgccgcgcct ccgcgccgaa
     4561 gtcggccgag gtctggggcg gccgcacgag ctggtcgacg gccgccccct cgacgaacgt
     4621 ctgccgcagg aacgagtcgt tggcagtgtt ctcgagcaga cgccgcacca ggtacgccat
     4681 gccggggatg agctcgccca ccggcgcgta ctcgcgcagg cgcagaccca tcccgcccac
     4741 ggccgccttg atcgggtcgc ccatgccgtg gagcatctgg agctcgaacg cgtcccgtga
     4801 cagccccagg ccccgggcgg tctccacggc gaccgccagt gagcggacgt tgtggctggc
     4861 gaacgccggc cggacgacgt ccgcggcctc cagcatctcc cgtgtgaggc gttcgtacat
     4921 ggcgtcggtg tcgggcttgt gcgtgaacac cggcaccggc cacccctcct gcgcggcgat
     4981 gaccgtctcg tagtcccagt aggcgccctt gaccagccgc aggttgaacg gccggccacg
     5041 ctcgcgcgcc cacgtgacga gccgcgtgag gtcctggtcg gcgtcgcgca ggtacgcctg
     5101 caggacgacg ccggcgtcgc ggtagtcgcg gaactcgccc tcgtcgagga gggacgtgaa
     5161 gaccgcgagc gtcaggtcgc ggtggcggaa ctgctcgagg tcgagcgtca gcgccgcccc
     5221 cgtcgcgacg gccttgcgga agatcggccg gagcaggtcc ttgacggcga cgacgctccc
     5281 accgaagtcc gcggggtcga tctgcgagta caaggaggtg atcttgaggc tgaggttgac
     5341 gcgcgggagc ggcccccagg cggcttcgtc gatcacgggg acgcggggcc acgaggcggc
     5401 ctcggtggcc aggccgtcga ggagtccgag gtaggtgcgc tggtaggcga ggccctcagc
     5461 ttcgctgacc gtggcctcgc cgagcacgtc gagggtgaac ccgatgccgt cacggcgcag
     5521 cgcgcgcagg gccgggatgg cgccgcgcgc gtcccggccg acgatgaagc gctgcgcgaa
     5581 ccccttcatc tggctgcgga tcacggcgct cgcgaggggc gccaccggcg agcgctgccc
     5641 ggtgaggctg atgccccagc gcagcggggc cggcgcggcg acaccctccc gggcgaagta
     5701 ctcgcgcagg tggcgggcga tctcgggccg gctgcgcagg gtggggaaga cgtccacga
//
LOCUS       DASUMX010000054         2073 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_2028322, whole genome shotgun sequence.
ACCESSION   DASUMX010000054 DASUMX010000000
VERSION     DASUMX010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2028322"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..1121)
                     /locus_tag="VFZ86_02260"
     CDS             complement(144..1121)
                     /locus_tag="VFZ86_02260"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEX5641144.1"
                     /translation="MPEHNGLDGKLMSVGEAVGRFVHDGDQVCVGGFTINRNPMAVVY
                     EIARRRVKDLHLVAHSNGQALDVLVGAGCVRRLDIAYGGNGRFAPTCIRFRKAVERGE
                     LQVEDYTNNMMSLRFLAGALNIPFIPSKSGLGTDVVERSGFPPELRSEDGVPCEKVIV
                     IDDPFGHEDDEVVLLPALTPDVSVIHAQQVSTDGTVRIKGLTFADLEQCKASTAVIVT
                     CEEIVPESYLRLDPDQNALPPFLVDAVVPVPYGAHPTACRYFYDYDPAHLNHYKKAAR
                     DDDAFAAWLQEWVYGVESWDDYLDKVGGAQLARIRANTVAGYAPGLDRR"
     gene            1372..1755
                     /locus_tag="VFZ86_02265"
     CDS             1372..1755
                     /locus_tag="VFZ86_02265"
                     /inference="COORDINATES: protein motif:HMM:NF024388.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3870 domain-containing protein"
                     /protein_id="HEX5641145.1"
                     /translation="MSAPPTTFLFSGYARLPQDVSHQAMYRRVGVVIEVDDKGLVVAC
                     STTLMMPVASDFLERLLVGRNVLTERQEIEALVRYRYRGHSQGALVSALHKAFEAVDQ
                     SPLVLGEAAGPLPSARPDGTAEERG"
BASE COUNT          287 a          727 c          745 g          314 t
ORIGIN      
        1 catcgccacg cctgtcccgg cgaagagcac gtcgccgtcc ttgatgaagc ggccggcggc
       61 gatcgccatc atctcgcgcg cggtgtaagc cggcgcggcg ggagccgaag cggcctcgtg
      121 ggctgtcgtg gactccatcg cgatcacctc cggtccaggc ctggcgcgta cccggcgacg
      181 gtgttggcgc ggatgcgtgc cagttgcgcc cccccgacct tgtccaggta gtcgtcccag
      241 ctctccacgc cgtacaccca ctcctggagc caggcggcga acgcgtcgtc gtcccgggcg
      301 gccttcttgt agtggttgag gtgcgcgggg tcgtagtcgt agaagtagcg acaggccgtc
      361 gggtgcgcgc cgtaggggac gggcaccacc gcgtcgacca ggaagggcgg cagcgcgttc
      421 tggtcggggt cgaggcgcag gtagctctcg gggacgatct cctcgcaggt gacgatgacc
      481 gccgtcgacg ccttgcactg ctccagatcg gcgaaggtga ggcccttgat gcgcacggtg
      541 ccgtcggtgc tgacctgctg ggcgtggatg acggagacgt cgggcgtgag cgcgggaagc
      601 aggacgacct cgtcgtcctc gtggccgaac gggtcgtcga tgacgatgac cttctcacaa
      661 gggacgccgt cctcggagcg aagctcgggc gggaagccgg agcgctccac gacgtccgtt
      721 cccagcccgg acttgctcgg gatgaagggg atgttcagcg ctccggccag gaaccgcagg
      781 ctcatcatgt tgttggtgta gtcctcgacc tgcagctcgc cccgctccac ggccttgcgg
      841 aagcggatgc aggtcggcgc gaagcggccg ttgccgccgt aggcgatgtc gaggcggcgc
      901 acgcagcccg ccccgacgag cacgtcgagc gcctggccgt tggagtgcgc caccaggtgc
      961 aggtccttga cccggcgccg ggcgatctcg tagacgactg ccatcgggtt gcggttgatg
     1021 gtgaagccgc cgacacagac ctgatcgccg tcgtggacga agcgtccgac ggcctcgccg
     1081 acgctcatca gcttgccgtc cagcccgttg tgctctggca tcgcgtcccc ctcgtcgagt
     1141 gcgccggggt ccccttaacg cgaacgaccc cagcgagcac ggtgcgtctc agctggggtg
     1201 cagggcctga cggaggggcc ggtgggaacg ccgccggccc gggccttgcg gcagcggcac
     1261 catgatacac gcgggcggcg gcggcgcaac tgccgccctc cgccgccggg cgcccgtgcg
     1321 gcggccgcgg ggcgaggcct cgcgcgcacg agcctgtgct aaagtgcgcg catgagcgcg
     1381 ccgcccacga ccttcctgtt ctccggctac gcccggctgc cgcaggacgt cagccaccag
     1441 gcgatgtaca ggcgcgtcgg cgtggtcatc gaggtggacg acaagggtct ggtcgtcgcc
     1501 tgcagcacga cgctcatgat gccggtcgcc tccgacttcc tcgagcgcct gctcgtcggc
     1561 cgcaacgtgc tcaccgaacg gcaggagatc gaggccttgg tgcggtaccg ctatcgcggc
     1621 cactcacagg gcgcgctggt ctccgcgctg cacaaggcct tcgaggccgt cgatcagagc
     1681 ccgctggtgc tcggcgaggc cgcgggtcct ctgccaagcg cgcgtccgga cggcacagcg
     1741 gaggagcgcg gctagcgcgc gccgctgtcg tggcggcccg atacgatccg tcgtagactc
     1801 tcggtcgacc ggtcggtcac caggcctggg aggagtgtga cacgcagggg gccatctggc
     1861 gacgcgacgc gcgcgtcccg cgcatcgggc accgccgcgc cccccggacg accggcacgc
     1921 tcccatcgcg ggccggcgaa gttccgtccg cccacgatgc gcgtggagct cgtcgagcgc
     1981 cgctccctca tcacgacgct ctctggtacc gccgagccct tggtcctcct ctgcgccccc
     2041 gcggggaccg gcaagacgac aacgctcgcg ttg
//
LOCUS       DASUMX010000055         4483 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1740679, whole genome shotgun sequence.
ACCESSION   DASUMX010000055 DASUMX010000000
VERSION     DASUMX010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4483
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1740679"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..908
                     /locus_tag="VFZ86_02270"
     CDS             <1..908
                     /locus_tag="VFZ86_02270"
                     /inference="COORDINATES: protein motif:HMM:NF016218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF459 domain-containing protein"
                     /protein_id="HEX5641146.1"
                     /translation="FFLAGLLDARAIEKDVQARPYGALRTLQLALLAPMTGLSGLVRA
                     DDLGAAVAGALGRGEEEHHTLAEVKKTKKPLWPRPITEERPLRLYVAGDSMDQVFGSS
                     LVNLAEETGLVKGKNDYHVSSGLSRPDFYDWPQRLVDQIVVFRPDAAVVLFGANDGQN
                     VLHQGEVLKVGTKAWQEVYAARVGEAMDILSTRGRRVYWVGNPIMKDFGYRERIAMMN
                     HIYEAEAKKRPGVTFVSTWRALANEKGSYTEYLKDENGDQVLMRAPDGIHLTRAGGDR
                     MAQVVLDAIVKEWGMAGAGSPSPAP"
     gene            complement(912..1865)
                     /locus_tag="VFZ86_02275"
     CDS             complement(912..1865)
                     /locus_tag="VFZ86_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein HesB"
                     /protein_id="HEX5641147.1"
                     /translation="MKSVVLIALRGAGGEPVDLRRTLGSHGVAELPPLQVDTDRRGAL
                     LTLATPEGARTVRLREAAGPRAGVPAVAVDIADAGPPPAPPAAAALAATLRRMLCLDD
                     DLSGFYAKVAGDPDLAWAARGAGRLLRSPTVFEDVVKTVCTTNCAWSATERMVGALVG
                     RLGEPAPGAPPDGVAGRSFPTPEAMAAAADGFYAAVARAGYRGRYLREIARRVVSGEL
                     DLEELLVAGRDELPDEEVERRLLGLPGVGPYAAAHVMLLLGRHSRLVLDSWTRPKYAR
                     LLGRPAGKLVADATIARRFRRYREHAGLAFWLVLTRDWMGD"
     gene            complement(1862..2839)
                     /locus_tag="VFZ86_02280"
     CDS             complement(1862..2839)
                     /locus_tag="VFZ86_02280"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX5641148.1"
                     /translation="MLGIALALAASVCWGSGDFLGGFSTRRASLWAVIIGSQAVGLAG
                     AALVTLGTGHGWPGLQAVWPVLLGGLASVVAISCFYKALAIGTMSIVAPISATNALIP
                     FAVGIAAGERPATVQLAGVALAAVGVILVAVEPARSGATIGAAGLPGEPAVDPAGAAA
                     LFRPGRASGEAALPATATRGHVARPARRDQRRAIALALTAAVCMGLVLVGYDATSRYD
                     PLWAMLGGRLSSAVLFAVVFAVLRPHVKVERSALPLIVAVGILDTGANGLFALATTEG
                     YLSLVGVLGSLYPVVTVLLAFGVLRERIAPHQMAGAAATLAGVALIAAG"
     gene            2934..3236
                     /locus_tag="VFZ86_02285"
                     /pseudo
     CDS             2934..3236
                     /locus_tag="VFZ86_02285"
                     /inference="COORDINATES: protein motif:HMM:NF012684.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
     gene            3246..4058
                     /locus_tag="VFZ86_02290"
     CDS             3246..4058
                     /locus_tag="VFZ86_02290"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX5641149.1"
                     /translation="MAVVIGVLLVAFVAGVFGSMLGVGGGIIMVPVLSLAFGVPIKTA
                     IATSIICVIATSSMAQTTFAARGMTNMRLGMFLEVATTVGALAGGITAVLIDGGILQA
                     CFAVLLFYVAWQMSRRRGDVVPVEGPVMPSSYYDPAQGRQVSYGVHGLGAGFALSVVA
                     GNVSGLLGVGGGAFKVPIMNLLMGVPLKATIATSNLMIGVTAATSAAIFYGRGYVDPR
                     WAVPAALGILVGARIGPRFAIHLPARILVLIFEVVLVVFGLLMLLKALGVGV"
     gene            4055..4411
                     /locus_tag="VFZ86_02295"
     CDS             4055..4411
                     /locus_tag="VFZ86_02295"
                     /inference="COORDINATES: protein motif:HMM:NF019459.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1634 domain-containing protein"
                     /protein_id="HEX5641150.1"
                     /translation="MNDRPAHVMDPIERLVSRVLGVGIAVAVALMLSGLVLGVAKGEG
                     VPAHVVPLADLLTGLTALDPAAWLSAGLLVLVATPFVRVGGSVVTFAVERDRRYVLVT
                     AVVLAVMCLGVLLGRV"
BASE COUNT          597 a         1674 c         1651 g          561 t
ORIGIN      
        1 gcttcttcct cgccgggctg ctggacgcgc gcgccatcga gaaggacgtc caggcgcgcc
       61 cctacggcgc gctgcgcacg ctgcagctcg ccctgctcgc gccgatgacg gggctgagcg
      121 ggctcgtgcg cgccgacgac ctcggcgccg ccgtggccgg cgcgctcggc cgcggcgagg
      181 aggagcacca caccctcgcc gaggtcaaga agacgaagaa gccgctctgg ccgcgcccga
      241 tcaccgagga gcggccgctg cgcctgtacg tggccggcga ctcgatggac caggtcttcg
      301 gctcgtcgct cgtcaacctc gcggaggaga cggggctggt caagggcaag aacgactacc
      361 acgtctcgag cggcctcagc cggccggact tctacgactg gccgcagcgc ctcgtcgacc
      421 agatcgtcgt cttccggccg gacgcggccg tcgtcctctt cggcgccaac gacggccaga
      481 acgtcctcca ccagggcgag gtcctcaagg tcgggaccaa ggcctggcag gaggtctacg
      541 ccgcccgcgt gggcgaggcg atggacatcc tgagcacccg cgggcgcagg gtgtactggg
      601 tgggcaaccc gatcatgaag gacttcggct accgcgagcg catcgcgatg atgaaccaca
      661 tctacgaggc ggaggcgaag aagcgcccgg gggtcacgtt cgtgagcacg tggcgcgcgc
      721 tggcgaacga gaagggctcc tacacggagt acctgaagga cgagaacggc gaccaggtcc
      781 tgatgcgcgc ccccgacggc atccacctca cgcgcgccgg aggcgaccgc atggctcagg
      841 tcgtgctcga cgcgatcgtg aaggagtggg ggatggccgg agccggctcg cccagtcctg
      901 ccccctgatc ctcagtcgcc catccagtcc cgggtcagca ccagccagaa ggcgagcccg
      961 gcgtgctcgc ggtagcggcg gaagcgccgg gcgatcgtcg cgtcggcgac cagcttcccg
     1021 gccggccgtc cgagcagacg cgcgtacttg ggccgcgtcc aggagtcgag gaccaggcgg
     1081 gagtggcggc ccagcagcag catgacgtgg gccgccgcgt acggcccgac gcccggcagg
     1141 ccgaggaggc ggcgctcgac ctcctcgtcg ggtagttcgt cgcgtccggc gacgagcagc
     1201 tcctcgaggt ccagctcgcc cgagacgacg cgccgcgcga tctcccggag gtaccggccg
     1261 cggtagccgg cgcgtgcgac ggcggcgtag aagccgtcgg cggcggcggc catggcctcc
     1321 ggcgtcggga aggaccgccc tgcgacgcca tcgggcggcg cccccggggc cggctctccg
     1381 agccggccga ccagggcgcc gaccatgcgc tccgtcgccg accaggcgca gttcgtcgtg
     1441 cagacggtct tgaccacgtc ctcgaagacc gtcgggctcc gcagcagccg tccggcgccg
     1501 cgcgccgccc aggccaggtc cggatcgccg gcgaccttgg cgtagaagcc gctcaggtcg
     1561 tcgtccaggc agagcatgcg ccgcagcgtc gcggccaggg ccgccgcggc cggcggcgcc
     1621 ggcggcggcc ctgcgtcggc gatgtccacc gcgacggcgg gcacgccggc gcgcggtccc
     1681 gccgcctccc tgagccgcac cgtccgcgcc ccctccggcg tcgccagggt gagcagcgcg
     1741 ccgcgccggt cggtgtcgac ctgcagcggc ggcagctcgg cgacgccgtg cgagcccagc
     1801 gtgcgccgca gatcgaccgg ttcgccgccg gcgccgcgca gcgcgatgag gaccacactc
     1861 ttcacccggc ggcgatgagg gcgacgccgg cgagggtcgc cgccgcgccg gccatctggt
     1921 ggggggcgat gcgctcgcgc agcacgccga acgcgagcag caccgtgacg accgggtaga
     1981 gcgagccgag cacgccgacc aggctgaggt agccctcggt cgtggcgagc gcgaacaggc
     2041 cgttggcgcc ggtgtcgagg atgccgacgg cgacgatgag cggcagcgcc gagcgctcga
     2101 ccttgacgtg cggccgcagc accgcgaaca cgaccgcgaa gagcacggcg gacgagagcc
     2161 tgccgccgag catggcccac aacgggtcgt agcgcgaggt ggcgtcgtag ccgacgagga
     2221 ccaggcccat gcagacggcg gccgtgagcg ccaaggcgat ggcgcgtcgc tggtcgcgcc
     2281 gcgccgggcg ggccacgtgg ccgcgtgtgg ccgtcgcggg cagggcggcc tcgccgctcg
     2341 cacgcccggg gcggaacagc gccgccgcgc cggccgggtc caccgccggc tcgccgggca
     2401 gccccgccgc cccgatcgtc gcgccgctgc gcgccggctc gaccgccacc aggatcacgc
     2461 cgacggcggc cagcgcgacg ccggcgagct ggaccgtggc cggccgctcg ccggcggcga
     2521 tgccgacggc gaaggggatc agggcgttgg tggcgctgat cggcgcgacg atgctcatcg
     2581 tgccgatggc gagcgccttg tagaagcagg agatggcgac cacgctggcg agcccgccga
     2641 gcagcaccgg ccacacggcc tgcagccccg gccagccgtg acccgtgccg agggtcacca
     2701 gcgccgctcc cgcgaggccc acggcctggc tgccgatgat caccgcccag aggctggcgc
     2761 gccgggtgct gaagccgccg aggaagtcgc cggagcccca gcacacggac gcggcgaggg
     2821 cgagggcgat gccgagcatg cccagaggct atcacgcggt ggtgtacgct ccctgcatgg
     2881 acgagccgat cgacatcccc gagcgcgacg acgcgctcct cgcgcagtgc gacgtgcaca
     2941 cgttccgcgc cggcggcccc ggcggccagc accagaacgt caccgacagc gccgtgcggc
     3001 tggtgcaccg gcccaccggc gtcaccgtga cctgcagagc ccagcgcagc cagtacctca
     3061 acaagatgga cgcgctgcgc cgcctgcgcc tcaagctgcg gcgactcaat gcgccgccgc
     3121 ctccgccgcg ccgccccacg cggccctcgc gggcggcgac ggagcggcgc ctcgccgaga
     3181 agtcgcggcg ggcggtcgcc aagcgcctcc gccgcccgcc ctcgggcgac gagtaggtcc
     3241 ggcacgtggc agtcgtcatc ggcgtcctgc tcgtggcctt cgtcgccggc gtcttcggga
     3301 gcatgctcgg cgtcggcggc ggcatcatca tggtccccgt cctcagcctc gccttcgggg
     3361 tgcccatcaa gacggcgatc gcgaccagca tcatctgcgt catcgccacg tcctcgatgg
     3421 cccagacgac gttcgccgcg cgggggatga ccaacatgcg cctgggcatg ttcctcgagg
     3481 tcgccaccac cgtcggcgcg ctggccgggg gcatcacggc ggtgctcatc gacggcggca
     3541 tcctgcaggc gtgcttcgcc gtcctgctct tctacgtggc ctggcagatg agccgccgcc
     3601 gcggcgacgt cgtgccggtg gagggcccgg tgatgccgtc gagctactac gacccggcgc
     3661 agggccggca ggtcagctac ggcgtgcacg gcctcggcgc cggcttcgcg ctgtccgtcg
     3721 tcgccggcaa cgtctccggg ctgctcggcg tcgggggcgg ggccttcaag gtgccgatca
     3781 tgaacctgct catgggcgtg cctctcaagg ccaccatcgc caccagcaac ctcatgatcg
     3841 gcgtcaccgc cgccaccagc gcggcgatct tctacggccg cggctacgtg gacccgcgct
     3901 gggccgtccc ggccgcgctc ggcatcctgg tcggggccag gatcgggccg cggttcgcca
     3961 tccacctgcc ggcgagaatc ctcgtcctga tcttcgaggt cgtcctcgtg gtcttcggcc
     4021 tgctcatgct cctcaaggcg ctgggggtgg gcgtgtgaac gaccggccgg cgcacgtcat
     4081 ggatcccatc gagcgcctcg tgagccgcgt gctcggcgtg ggcatcgccg tcgccgtggc
     4141 gctcatgctg tccggcctgg tcctgggcgt cgcgaagggt gagggcgtac cggcccacgt
     4201 cgtgcctctg gcggacctcc tgaccggact gacggcgctc gaccccgccg cctggctctc
     4261 cgcgggactg ctcgtgctcg tcgcgacgcc gttcgtgcgc gtgggcgggt ccgtcgtcac
     4321 cttcgccgtg gagcgggacc gccgctacgt cctcgtgacg gcggtcgtcc tcgcggtgat
     4381 gtgcctcggc gtcctgctcg gccgggtgtg acgcgccggc ccggctacgg ggcgcaggag
     4441 ggcgccacgt cgagtcccct gaccgccgcg gccgcccgcc ttc
//
LOCUS       DASUMX010000056         4183 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1021357, whole genome shotgun sequence.
ACCESSION   DASUMX010000056 DASUMX010000000
VERSION     DASUMX010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1021357"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..733
                     /gene="mtnA"
                     /locus_tag="VFZ86_02300"
     CDS             <1..733
                     /gene="mtnA"
                     /locus_tag="VFZ86_02300"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HEX5641151.1"
                     /translation="APGSTPAAVAGALLARAQAIHDDDVDRCLRIGEHGAALFKAGAR
                     ILTHCNAGALATAGYGTALGVIRAAAARYPDISVWVDETRPWLQGARLTAWELQVEGI
                     PYRLISDSMAGYFMRRGEVDGVVVGADRIAANGDTANKIGTYSVSVLAKEHGVPFYVA
                     APLSTVDMTIADGEAIPIEERDPAEVTAFRGEQVAPDGAVARHPAFDVTPAAGIAAIV
                     TEAGVLRAPVGAALASVCTAGGCRP"
     gene            730..1860
                     /locus_tag="VFZ86_02305"
     CDS             730..1860
                     /locus_tag="VFZ86_02305"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HEX5641152.1"
                     /translation="MTVRAYLMAAGLGTRLYPLTGLTSKPMVPILNRPVMEHLLRRLR
                     RHGVTEIAANLHYHPDKISTYFGDGREFGVDLRYNLEEQLMGTAGGASAFREFLGDST
                     FLVMSGDGLTDVDLSAFIAAHKRSGGVATLAVKEVEDPSLYGVVVHDEGMRVTGFQEK
                     PAREEARSHLCNCGIYAFEPRIFDYVTPGAFVDWAKDVFPALLDDGLPFHCWELQSYW
                     NDVGNIEQYRLSNFDALLGRVDVDIPGRRIASGVWVGQGTEIESGARLEPPVLLGAGC
                     LVEAGAELIGPLIIGDGCVVERGAVLEGVIHWDGAKAGRDSRLAGSILGRNVVVHHEA
                     VVHEDAVIGDRSEVAAHALVDAGARLEPHTLFGPGGRGGTGE"
     gene            1866..2690
                     /locus_tag="VFZ86_02310"
     CDS             1866..2690
                     /locus_tag="VFZ86_02310"
                     /inference="COORDINATES: protein motif:HMM:NF039185.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double zinc ribbon domain-containing protein"
                     /protein_id="HEX5641153.1"
                     /translation="MPRVHAGGALDGLLDLVFPRRCVVCRAAGDWLCATCAADLRPLP
                     DDRCRRCGAPLRRAGRPARPLRAGSSGHGLPACRECAGRGLAFSAASAAFCYEGPARA
                     LVTACKFRALRTLSDELAGRAAPAFAAAALRAGAEAVVTSVPAHRDHRLDRGFDLAES
                     LARRLAAGAGLTYAPLLRRVRHGARQSGLDRATRAANVHQAFALRQSGIGVRNRLKRV
                     IIIDDVYTTGETLNQCAEALATASHDDPHVFTFARTVRATPAQASRDHAVHKERCR"
     gene            2687..3307
                     /gene="raiA"
                     /locus_tag="VFZ86_02315"
     CDS             2687..3307
                     /gene="raiA"
                     /locus_tag="VFZ86_02315"
                     /inference="COORDINATES: protein motif:HMM:NF014534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEX5641154.1"
                     /translation="MKTQIKGRNVTVTSALQDYANEKIEHVHKLLQQRKIDEVTRVEL
                     ELKVEKNPSIPEPCIAEATVFTRGPVIRARESSTDMYAAIDLVTDKLVRRVKKYHDKI
                     HGKTKHGHEKIVAPVEPAPELAPVAAATALADELAGEAEHVSDNGRIVKTKQFALKPM
                     SINEATLQMELVGHDFFVFTNAESNRTNVVYRRNDGHYGLIEPAEN"
     gene            3551..>4183
                     /locus_tag="VFZ86_02320"
     CDS             3551..>4183
                     /locus_tag="VFZ86_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010949934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEX5641155.1"
                     /translation="MVDKLLHAGEGRRLKTLQDQTQRVTELEPTVAGLSDDQLRAKTA
                     EFRQRHENGEMLDDLLFEAFAVAREAAKRALGMRPFDVQVLGGIVLHDGDIAEMKTGE
                     GKTLVATMPMYLNGIAGNGAHLVTVNDYLAKRDADWMGQVYRFLGLEVGVIQANMTPE
                     ERRAQYNADVTYGTNSEFGFDYLRDNMTMRREHMVQRGHHYCIVDEVDSIL"
BASE COUNT          652 a         1542 c         1428 g          561 t
ORIGIN      
        1 cgcgcccggc tccacgccgg cggccgtcgc cggcgcgctg ctcgcacgcg cgcaggccat
       61 ccacgacgac gacgtcgacc gctgcctgcg catcggtgag cacggcgccg cgctcttcaa
      121 ggccggcgcc cgcatcctca cccactgcaa cgccggcgcc ctggccacgg cgggctacgg
      181 caccgccctc ggcgtgatcc gggcggcggc ggcgcggtac ccggacatct ccgtctgggt
      241 cgacgagacg cggccgtggc tccagggcgc caggctcacc gcctgggagc tccaggtcga
      301 gggcatcccg taccggctca tcagcgacag catggccggc tacttcatgc gccgcggcga
      361 ggtcgacggc gtcgtggtcg gcgccgaccg catcgccgcc aacggcgaca ccgccaacaa
      421 gatcggcacc tacagcgtca gcgtgctcgc caaggaacac ggcgtgccct tctacgttgc
      481 ggcgccgctc tccaccgtcg acatgacgat cgcggacggc gaggcgatcc ccatcgagga
      541 gcgcgacccg gcggaggtca ccgcgttccg cggcgagcag gtcgcgcccg acggcgccgt
      601 cgcgcgacac cccgccttcg acgtcacccc ggccgccggc atcgccgcca tcgtcaccga
      661 ggccggcgtg ctgcgcgcgc ctgtcggcgc ggcgctggcg tcggtgtgca cggcgggggg
      721 gtgccgcccg tgacggtgcg cgcctacctc atggccgcgg gcctcggcac ccggctgtac
      781 ccgctcaccg ggctcacgtc caagcccatg gtgccgatcc tcaaccggcc ggtcatggag
      841 cacctcctgc gccggctccg tcgtcacggg gtcaccgaga tcgccgccaa tctgcactac
      901 caccccgaca agatcagcac ctacttcggt gacggcaggg agttcggcgt cgacctccgc
      961 tacaacctcg aagagcagct catgggcacc gccggcggcg ccagcgcgtt ccgtgagttc
     1021 ctgggcgatt cgaccttcct cgtgatgagc ggcgatgggc tcaccgacgt cgacctcagc
     1081 gccttcatcg cggcgcacaa gcggtcgggc ggggtcgcca cgctggcggt caaggaggtg
     1141 gaggacccgt cgctgtacgg cgtggtcgtg cacgacgagg gcatgcgggt caccggcttt
     1201 caggagaagc ccgcgcgcga ggaggcgagg tcgcacctct gcaactgcgg catctacgcg
     1261 ttcgagccgc ggatcttcga ctacgtcacc ccgggcgcct tcgtggactg ggcgaaggac
     1321 gtcttcccgg cgctgctgga cgacgggctg ccgttccact gctgggagct ccagagctac
     1381 tggaacgacg tcggcaacat cgagcagtac cgcctgagca acttcgacgc cctgctcggc
     1441 cgggtggacg tggacattcc cggccgccgg atcgcctcgg gcgtgtgggt gggccagggc
     1501 accgagatcg agagcggcgc gcggctcgag ccgccggtcc tcctcggcgc cggctgcctc
     1561 gtcgaggccg gcgccgagct catcggcccg ctcatcatcg gcgacggctg cgtggtcgag
     1621 cggggcgcgg tgctcgaggg cgtcatccac tgggacggcg ccaaggccgg ccgggactct
     1681 cgtctcgccg gcagcatcct cggccgcaat gtcgtcgtgc accacgaggc cgtcgtccac
     1741 gaggacgccg tcatcggcga ccgctccgag gtggcggcgc acgcgctcgt cgacgccggc
     1801 gcgcggctcg agccgcacac tctcttcggc cccggcggcc gcggcggtac cggggagtga
     1861 gcgccgtgcc gcgggtgcac gccgggggcg cactcgacgg cctgctggac ctcgtcttcc
     1921 cgcggcgctg cgtggtgtgc cgcgcggccg gcgactggct ctgcgcgacc tgcgccgccg
     1981 acctgcggcc cctgcccgac gaccgctgcc ggcgctgcgg cgcgccgctg cgccgcgccg
     2041 gccggccggc gcggccgctg cgcgccggct cctccgggca cggcctgccg gcgtgccgcg
     2101 agtgcgccgg ccgcggtctc gcgttctcgg cggcctcggc ggcgttctgc tacgaggggc
     2161 cggcgcgagc gctcgtcacc gcctgcaagt tccgcgcatt gcgcacgctc agcgacgagc
     2221 tcgccgggcg cgcggcgccg gccttcgcgg ccgccgccct ccgggccggc gccgaggccg
     2281 tcgtcacctc cgtgccggcg caccgcgacc accggctcga ccgcggtttc gacctcgccg
     2341 agtcgctcgc ccgccggctt gcggccggcg ccggactcac ctatgctccg ctgctccgcc
     2401 gcgtgcgcca cggcgcccgc cagagcgggc tcgaccgggc gacgcgcgcg gccaacgtcc
     2461 atcaagcctt cgcgttgcgc cagagcggca tcggggtacg taatagactc aagcgagtga
     2521 tcatcattga cgacgtctac accacaggtg aaacgttgaa ccagtgtgcg gaagcgctcg
     2581 ctaccgccag tcacgacgac ccccacgtct tcacctttgc gcgtacagtt cgtgctacgc
     2641 ctgcccaagc atcgcgagac cacgccgtcc acaaggagcg atgtcgatga aaacccagat
     2701 caagggccgc aatgtcaccg ttacgtccgc cctgcaggac tacgccaacg agaagatcga
     2761 gcacgtccac aagctcctgc agcagcgcaa gatcgacgag gtcacgaggg tagagctcga
     2821 gctcaaggtc gagaagaacc cgagcatccc cgagccgtgc atcgccgagg cgacggtgtt
     2881 cacacgcgga cccgtgatca gggcgcgcga gtcgtccacc gacatgtatg ccgccatcga
     2941 cctggtgacc gacaagctcg tgcgccgcgt caagaagtac cacgacaaga tccacggcaa
     3001 gaccaagcat ggacacgaga agatcgtcgc gccggtcgag ccggcgcccg agctcgcgcc
     3061 ggtcgccgcc gccacggccc tcgccgacga gctcgccggc gaggcggagc acgtgagcga
     3121 caacgggcgc atcgtcaaga ccaagcagtt cgcgctcaag cccatgtcga tcaacgaggc
     3181 caccctccag atggagctcg tcggccacga cttcttcgtg ttcaccaacg ccgagtccaa
     3241 ccggacgaac gtcgtgtacc gccgcaacga cgggcactac ggcctcatcg agccggcgga
     3301 gaattgaaag acgggagagg actcaatcga gcggtgcgcc gcaagggcac cgctcaactg
     3361 aaggacggaa gcggactcaa tcgagcggtg cgccgcaggg acaccgctca ggactgaggc
     3421 gggagtcagg gctggtcgcg cgtccgcgcc ggcgcggtgg gcggcgcggg cttcgcgcga
     3481 gggcgcgctg tgcgacaatg ggcgtagacc tcatctccga cactccggga gtcgtgttcc
     3541 agtgatcaag atcgtcgaca agcttctgca tgccggtgaa ggccggcgcc tcaagacgct
     3601 ccaggatcag acccagcggg tcacggagct ggagcccacg gtcgccggtc tgagcgacga
     3661 ccagctgcgc gccaagaccg ccgagttccg ccagcggcac gagaacggcg agatgctcga
     3721 cgacctgctc ttcgaggcgt tcgcggtcgc gcgcgaggcg gcgaagcgcg cgctcggcat
     3781 gcgtccgttc gacgtccagg tgctgggcgg gatcgtgctc cacgacggcg acatcgccga
     3841 gatgaagacc ggcgagggca agaccctcgt ggccacgatg ccgatgtacc tcaacgggat
     3901 cgccggcaac ggcgcccacc tggtcaccgt caacgactac ctcgccaagc gcgacgccga
     3961 ctggatgggc caggtgtacc gcttcctcgg cctcgaggtg ggcgtcatcc aggccaacat
     4021 gacccccgag gagcgccgcg cgcagtacaa cgccgacgtc acctacggca ccaactccga
     4081 gttcggcttc gactacctgc gcgacaacat gaccatgcgc cgcgagcaca tggtgcagcg
     4141 cggccaccac tactgcatcg tggacgaggt cgactcgatc ctc
//
LOCUS       DASUMX010000057         4645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_447672, whole genome shotgun sequence.
ACCESSION   DASUMX010000057 DASUMX010000000
VERSION     DASUMX010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4645
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447672"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="VFZ86_02325"
     CDS             complement(<1..287)
                     /locus_tag="VFZ86_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX5641156.1"
                     /translation="MIKVAEWGTGMMGQGLLGYILDRPKDIDLVGVIVTNPAKEGKTV
                     GELLGRPCDVAMTTDFEAVLARKPDVVCINTQSNLDEVEPQIIPALKAGCNV"
     gene            complement(678..1325)
                     /locus_tag="VFZ86_02330"
     CDS             complement(678..1325)
                     /locus_tag="VFZ86_02330"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEX5641157.1"
                     /translation="MTSPELVAEPFAAWLRATCATLERDVPADVPCGSCTACCRTFHQ
                     LHLRPGEKRARKRLPKDYLSVARGLPPGYLLLGYTDEGACPVLVHGRCTIYEDRPLVC
                     RTYDCRMYAATGIAPDRPEIAEQVRRWRFSHPTPADRELHEAVLAAVRFIREHPQCLP
                     SEAARRQPIRVATLAVAAHGKFLADATTGVRRRPVSERDRIFAFAGANEELFGDG"
     gene            complement(1322..2005)
                     /locus_tag="VFZ86_02335"
     CDS             complement(1322..2005)
                     /locus_tag="VFZ86_02335"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEX5641158.1"
                     /translation="MSDDDHCTRDLRDDGNGTRDLPAGDFGAWLARMRAALRHEADAD
                     VPCGECCACCSTAHFVHIGPEETETLAHVPPELVFPAPGLPAGHVVLGYDESGRCPLL
                     DERGRCTIYEQRPRTCRTYDCRVFAAAGIAADRDAISARVRRWRFACPAPADVDELAA
                     VHAAARFVREHAAAFPGAAVPRDPSQLAVLAVRVADVFLPGGPGAQGGDPAAVAAAVV
                     AAAACEEVP"
     gene            complement(2038..>4645)
                     /locus_tag="VFZ86_02340"
     CDS             complement(2038..>4645)
                     /locus_tag="VFZ86_02340"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX5641159.1"
                     /translation="AVAEVLAAREAYAAFLTTLAVAVADAFSRWAAATQLALGRLDFT
                     DLLGCLRDLLKREPAARAALQARFRYLLVDEFQDTDPLQAEIVFFLCEASPDAQDWRD
                     VVLQPGKLFVVGDPKQSIYRFRRADIGMYDQVKSLVAGQPGGTGAVEAISQNFRTTPA
                     VVQWVNNVFADVFDQDQEQGRQPAYQWVEPYRPAAEGPRVAVLLGDDLGTAAGAADAA
                     RRAEAQALAALLLAMQGEERHRWTVQDRAPGGGGEDEAWRPPRWGDVALLFRATTGLE
                     TYEQAFREAGVPYRVDGGKAYFARREVDDALLCLRAVDDPSDGPAVYGALHSTFFGFS
                     DDELFLFWAAGGRFDLFAAQPETHAAVAAALGTLRRLHERRAVCEPHELAVELVRRAR
                     TAEFLAATGSGGAQAIANLEKLVDRARAFSGAGGRGLGAFLAWAAEAGDASGEQESQV
                     DDDGDVVHLLTIHKAKGLEYPIVILAGGALGGGGGGGEPIVDREERRLAIRLKAELPG
                     APARDLEPQRYTVLRQREKLMEASEGRRLLYVAATRARDRLVVSCFGHLRNKDGSPAA
                     VLLGPIADARPAAGVLTEEYEDGGLLVLPPRVPPAVDDGDGAPSERDLAAARAEWRRR
                     REALLERAGVPAPATSPSGLEHVDDAVKTGGPGAPAGRARALALGSAVHRVMELCDLS
                     DAASIGPAATAAAAELERPDLAGEAADLAGACWRAAPVRAAAEAAAATGAVYRELPIG
                     ALVDGVVVGGAIDLVYRDGDEWVVVDYKTDRAAEPEVLRERYEPQGAAYALAVEAATG
                     AVVREVCFVAARADGLVVSVPVDDALRARARQEVGAAAAAGRALRPDELAAGEAHDGG
                     EAGRRADAGDAA"
BASE COUNT          590 a         1754 c         1719 g          582 t
ORIGIN      
        1 acgttgcagc ccgccttgag cgccgggatg atctgcggct cgacctcgtc gagattgctc
       61 tgcgtgttga tgcagaccac gtccggcttc ctggcgagca cggcctcgaa gtcggtggtc
      121 atcgcaacgt cgcacgggcg gcccagcagc tcgccgacgg tcttcccctc tttggccggg
      181 ttggtgacga tgacgccgac gaggtcgatg tccttgggcc ggtcgaggat gtaccccaga
      241 aggccctgcc ccatcatgcc ggtgccccac tcagcgacct tgatcatgtc tcgctcctgt
      301 ccacagtagt tggcgcccgc gggcgctggt tctcaggtat gcatacggaa tatgcagcac
      361 ggggcgggac tctgccgtgc gcctggcgac ggtctgggac tcgagacgga agcgggctgg
      421 ccgcctgtga cggccgtgca cggatgggtc cgcccggact gcgggcgggg atgcgccggg
      481 gcggcgcgcc tcagggcgcg ctcgaccggc gctcagatgc cggtggctgg aggaggctgg
      541 tcgaccgtgt cgtgcgcgag gatggaaagc tccttgccca tcgcgccccc ttcggtcgac
      601 tccggccggc gggtgccggc ggccttgcct cagggtatgc gggccggccg cgggccgtca
      661 accgtcggct tcaccgctca gccgtcgccg aagagctcct cattggcccc ggcgaaggcg
      721 aagatccggt ccctctccga gaccggcctg cggcgcacgc cggtggtggc gtccgcgagg
      781 aacttcccgt gcgcggcgac ggcgagcgtg gcgacgcgga tgggctgccg ccgggcagcc
      841 tcgctgggga gacactgcgg gtgctcccgg atgaaccgca cggcggcgag gaccgcctcg
      901 tgcagctccc ggtcggccgg cgtggggtgg ctgaagcgcc agcgccgcac ctgttccgcg
      961 atctcggggc ggtccggcgc gatcccggtc gccgcgtaca tcctgcagtc gtaggtgcgg
     1021 cacaccagcg gccggtcctc gtaaatggtg caccggccgt ggacgagcac cgggcaggcg
     1081 ccctcgtcgg tgtagccgag gagcaggtag cccggcggca ggcccctcgc cacggagagg
     1141 tagtccttgg gcagccgttt gcgcgcccgc ttctcgccgg gccgcaggtg gagctggtgg
     1201 aacgtccggc agcaggcggt gcagctcccg cagggcacgt cggccggcac gtccctctcc
     1261 agcgtcgcgc acgtggcccg cagccaggcc gcgaacggct ccgcgacgag ctccgggctc
     1321 gtcacgggac ctcctcgcag gcggcggctg cgaccaccgc cgcggcgacg gccgccgggt
     1381 cgcccccctg cgcaccaggg ccgccgggca ggaagacgtc cgcgacccgc acggcgagca
     1441 cggccagctg ggagggatcg cggggtacgg ccgcgccggg gaacgccgcc gcgtgctcgc
     1501 gcacgaagcg cgcggcggcg tgcacggcgg ccagctcgtc cacgtcggcc ggcgcggggc
     1561 aggcgaagcg ccagcgccgc acccgcgcgg agatcgcgtc cctgtcggcc gcgatacccg
     1621 ccgcggcgaa cacccggcag tcgtacgtgc ggcaggtgcg cggccgctgc tcgtagatgg
     1681 tgcagcggcc gcgctcgtcg agcagcgggc agcggccgct ctcgtcgtac ccgagcacca
     1741 cgtggccggc gggcagtccc ggagccggga agaccagctc gggggggacg tgcgcgaggg
     1801 tctccgtctc ttccggaccg atgtggacga agtgcgccgt ggagcagcag gcgcagcact
     1861 cgccacacgg cacgtccgcg tcggcctcgt gccgcagcgc ggcgcgcatg cgcgcgagcc
     1921 acgcgccgaa gtcgccggcg ggcaggtcgc gcgtaccgtt gccgtcgtcg cgcaggtcgc
     1981 gcgtgcagtg gtcgtcgtcg ctcacgcgtc agacgttgcc gccggggagg ctgcccgtca
     2041 agccgcgtct cccgcgtctg cccgtcgacc cgcctctccc ccgtcgtgcg cctccccggc
     2101 ggcgagctcg tcgggccgca gggcgcggcc cgcggcggcc gccgcgccca cctcctgcct
     2161 cgcccgtgcg cgcagcgcgt cgtcgacggg caccgagacc accagcccgt ccgccctggc
     2221 ggcgacgaag cagacctcgc gcacgaccgc gccggtggcc gcctcgacgg cgagcgcgta
     2281 ggcggcccct tggggctcgt agcgctcgcg caggacctcc ggctccgcgg cgcggtcggt
     2341 cttgtagtcg acgacgaccc actcatcgcc gtcgcgatac acgaggtcga tcgcgccgcc
     2401 gacgaccaca ccgtccacga gggcgccgat cggaagctcc cggtagacgg cgccggtggc
     2461 ggccgccgcc tcggcggcgg cgcgaacggg cgcagcccgc cagcacgcgc cggccaggtc
     2521 agccgcctcg ccggcgagat ccgggcgctc cagctccgcc gcggcggccg tggcggccgg
     2581 cccgatggac gccgcgtcgg agaggtcgca cagctccatc acgcggtgga cggcagagcc
     2641 gagagcgagc gcccgcgccc ggccggccgg ggcgcccggc ccaccggtct tgaccgcatc
     2701 gtcgacgtgt tcgaggccgc tcgggctggt ggccggcgca ggcacgccgg cacgctcgag
     2761 cagcgcctcg cgccgccgcc gccactctgc acgcgcggcg gccaggtcac gctcgcttgg
     2821 cgctccgtcg ccgtcgtcca cggccggagg gacgcgcgga ggcaacacga gcaggccgcc
     2881 gtcctcgtac tcctcggtca gcacgccggc cgcgggccgc gcgtcggcga tgggacccag
     2941 cagcacagcc gcgggggacc cgtccttgtt gcgcaggtga ccgaagcagg agacgaccag
     3001 acggtcccgc gcgcgggtgg cggcgacgta gagcagccgg cgaccctcgc tcgcctccat
     3061 gagcttctcg cgctgccgga gcaccgtgta gcgctgcggc tcgaggtcgc gcgccggcgc
     3121 gcccggcagt tccgccttga gcctgatcgc caggcgccgt tcctcgcgat cgacgatggg
     3181 ctcgccaccc ccaccgccgc cgcccagggc gccgccggca aggatcacga tcgggtactc
     3241 gagccccttg gctttgtgga tggtgagcag gtggacgaca tcaccgtcgt cgtccacctg
     3301 ggactcctgc tcgccggacg cgtcgcccgc ctccgcggcc cacgccagga acgccccgag
     3361 gcctcggccc cctgcgcccg agaaggcgcg cgcacggtcg accagcttct ccaggttggc
     3421 gatggcctga gcgccgccgg agccggtcgc ggcgaggaac tcggccgtgc gggcgcggcg
     3481 cacgagctca acggccagct cgtgcggttc gcagacggcg cggcgctcgt gcagccggcg
     3541 cagcgtgccg agcgccgcgg cgaccgccgc gtgcgtctcc ggctgcgccg cgaacaggtc
     3601 gaaccggccg ccggccgccc agaacaggaa cagctcgtcg tcgctgaagc cgaagaacgt
     3661 cgagtgcagc gcgccgtaca cggccggccc gtcgctcgga tcgtcgacgg cgcgcaggca
     3721 cagtagggcg tcgtcgacct cgcggcgcgc gaagtaggcc ttgccgccgt ccacccggta
     3781 cggcacgccg gcctcgcgga acgcctgctc gtaggtctcg agaccggtgg tcgcccggaa
     3841 gagcagggcg acgtcgcccc agcgcggcgg gcgccacgcc tcatcctcac cgccgccgcc
     3901 cggtgcgcga tcctgcacgg tccagcggtg ccgttcctcg ccctgcatgg cgaggagcag
     3961 cgcggccagc gcctgcgcct cggcccgccg cgccgcgtcc gcggcccctg cggccgtgcc
     4021 gaggtcgtcg ccgaggagca ccgccacccg cggcccctcc gcagccggac ggtagggctc
     4081 cacccactgg taggccggct ggcggccctg ctcctggtcc tggtcgaaca cgtccgcgaa
     4141 cacgttgttg acccactgca ccaccgccgg cgtggtgcgg aagttctggc tgatcgcctc
     4201 gaccgccccc gtgccacccg gctgccccgc gaccagggac ttcacctggt cgtacatgcc
     4261 gatgtccgcg cggcggaagc gatagatgga ctgcttggga tcgccgacca cgaacagctt
     4321 gcccggctgg aggaccacgt cgcgccagtc ctgcgcgtcc ggtgaggcct cgcagagaaa
     4381 gaagacgatc tccgcctgca gcgggtcggt gtcctggaac tcgtcgacca gcaggtagcg
     4441 gaagcgcgcc tgcagcgccg cacgggcggc cggctcgcgc ttgaggaggt cgcgcaggca
     4501 cccgagcaga tcggtgaaat cgaggcggcc gagcgccagc tgcgtcgcgg ccgcccagcg
     4561 cgagaacgcg tcggcgacgg cgacggcgag cgtggtgagg aaggctgcgt atgcctcccg
     4621 ggctgccagc acctccgcga ccgcc
//
LOCUS       DASUMX010000058         2891 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1022287, whole genome shotgun sequence.
ACCESSION   DASUMX010000058 DASUMX010000000
VERSION     DASUMX010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1022287"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1782
                     /locus_tag="VFZ86_02345"
     CDS             <1..1782
                     /locus_tag="VFZ86_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEX5641160.1"
                     /translation="MGSGGLIVMNEESCMVDVARYFLDFVQDESCGKCVPCRVGTKRM
                     LEIVERICNGEGEEGDIERLIALGETISETALCGLGQTAPNPVLSTIRHFRHEYEAHI
                     KDHYCEAGVCSTMFKARCSNACPASVNIPGFVSLVGVERYDDALKLHRERNPLASICA
                     RVCFHPCESKCMRSTLDGALAIRHVKRFMVEQETEPQLPEILENAENAARKVAVVGSG
                     PAGLSAAYFLCRLGYKPVVFEAEPKAGGMLVQAIPAYRLPRPELEREIAMIAAMGTRF
                     EYGKALGRDFTLQSLKDEGYEAVFVGVGAPQGMGIGVTGEDGPGVYDGLSFLKQYNVH
                     GTGEVGKNVAVIGGGNAAIDAARTALRLGAESVKILYRRTRAQMPAWGEEIDAADLEG
                     IDIMTLVAPEEILRDASGKVTGVRCKEMALGDYDRSGRRRPVAGHNPDFVVEADTVIA
                     AIGQKLDAAALMDGTAIELNRWGYLQADPNTGQTSAGWVFAGGDASTGPSSVVEAIGA
                     GERAAAGMDQHLTGANHAFWRRDIEPPTFFDPDADPELVERHAVEELAVADRVTNFDE
                     VELGWAAQVALAEARRCLRCDYGKYGS"
     gene            1800..>2891
                     /locus_tag="VFZ86_02350"
     CDS             1800..>2891
                     /locus_tag="VFZ86_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HEX5641161.1"
                     /translation="MPTLTIDDIKVEVPEGTTVLQAARQAGVKIPTLCFLDDVQSIGA
                     CRVCLVEVEGARTLVASCSQPATDGMVVKTNTGKVRDGRKMVVELILSEHDGDCKVCA
                     RSADCELRTLADELGIGDLRYEGEKTFRMIDDSTPALIRDTGKCISCRRCVTVCNEVQ
                     EVGGLFAQGRGFATLIGPAFSQNLSDVVCVQCGQCAAVCPVGAIQERNQIDEVWAALE
                     DPTKTVIVQTAPAIRAALGECFDYPAGTLVTGKMVAALRRLGFDAVFDTDFTADLTIM
                     EEGTELLGRLKKALVDGEEVALPQFSSCSPGWINYMEHFNPDMLANLSTCKSPQQMFG
                     ALAKTYYAEKIGVQPEDMVVVSVMPCTAKK"
BASE COUNT          473 a         1033 c          982 g          403 t
ORIGIN      
        1 atgggctcgg gcggcctgat cgtgatgaac gaggagagct gcatggtgga cgtggcccgc
       61 tacttcctcg acttcgtgca ggacgagtcg tgcgggaagt gcgtgccgtg ccgcgtgggc
      121 accaagcgca tgctcgagat cgtcgagcgc atctgcaacg gcgagggtga agagggcgac
      181 atcgagcgcc tcatcgccct cggcgagacg atcagcgaga cggcgctgtg cggcctcggc
      241 cagacggcgc cgaaccccgt gctctcgacg atccgccact tccgccacga gtacgaggcc
      301 cacatcaagg accactactg cgaggccggc gtctgctcca cgatgttcaa ggcgcgctgc
      361 tccaacgcgt gccccgcgag cgtcaacatc cccggcttcg tgagcctggt cggcgtggag
      421 cgctacgacg acgcgctcaa gctgcaccgc gagcgcaacc cgctggccag catctgcgcc
      481 cgcgtctgct tccacccgtg cgagagcaag tgcatgcgct cgaccctcga cggcgcgctc
      541 gcgatccgtc acgtcaagcg cttcatggtc gagcaggaga ccgagccgca gctgccggag
      601 atcctggaga acgccgagaa cgcggcccgc aaggtcgccg tcgtcggctc cgggccggcc
      661 ggcctctccg cggcctactt cctctgccgg ctgggctaca agccggtcgt cttcgaggcc
      721 gagcccaagg ccggcggcat gctcgtgcag gccatcccgg cctaccgcct gccgcgtccc
      781 gagctcgagc gtgagatcgc gatgatcgcg gccatgggca cgcgcttcga gtatggcaag
      841 gccctcggcc gcgacttcac gctccagagc ctgaaggacg agggctacga ggccgtgttc
      901 gtcggcgtcg gcgcccctca gggcatgggc atcggcgtca ccggtgagga cggccccggc
      961 gtgtacgacg ggctctcctt cctcaagcag tacaacgtcc acggcaccgg cgaggtcggc
     1021 aagaacgtcg ccgtgatcgg cggcggcaac gcggccatcg acgccgcccg caccgccctg
     1081 cgtctcggcg ccgagtcggt caagatcctc taccgccgca cgcgggcgca gatgccggcg
     1141 tggggcgagg agatcgacgc cgccgacctc gagggcatcg acatcatgac gctcgtggcc
     1201 ccggaggaga tcctccgcga cgcgagcggc aaggtcaccg gcgtgcgctg caaggagatg
     1261 gcgctgggcg actacgaccg cagcggccgt cgccgcccgg tggccggcca caaccccgac
     1321 ttcgtggtcg aggccgacac cgtcatcgcc gccatcggcc agaagctcga cgccgccgcc
     1381 ctcatggacg gcaccgcgat cgagctgaac cgctggggct acctgcaggc ggacccgaac
     1441 accggccaga cctcggccgg ctgggtgttc gccggcggcg acgcctccac cggtccgtcc
     1501 tcggtcgtcg aggccatcgg cgccggcgag cgtgccgcgg ccggcatgga ccagcacctg
     1561 accggcgcca accacgcgtt ctggcgccgc gacatcgagc cgccgacgtt cttcgacccc
     1621 gacgccgacc ccgagctcgt ggagcgccac gcggtcgagg agctcgcggt cgcggaccgt
     1681 gtcaccaact tcgacgaggt cgagctgggc tgggccgcac aggtggccct cgctgaggcg
     1741 aggcgctgcc tgcgttgcga ctacggcaag tacggctcgt gagcaaggag agcaagagga
     1801 tgccaaccct gaccattgac gacatcaagg tcgaggtgcc ggagggcacc acggtccttc
     1861 aggcggcccg acaggccggc gtgaagatcc ccaccctctg cttcctcgac gacgtccagt
     1921 ccatcggcgc gtgccgcgtg tgcctcgtcg aggtggaggg cgcaaggacg ctggtcgcct
     1981 cgtgcagcca gccggcgacc gacggcatgg tcgtcaagac caacaccggc aaggtccgtg
     2041 acggccgcaa gatggtcgtc gagctgatcc tctcggagca cgacggcgac tgcaaggtct
     2101 gcgcccggtc cgccgactgc gagctgcgca cgctcgccga cgagctcggc atcggcgacc
     2161 tgcgctacga gggcgagaag accttccgga tgatcgacga cagcaccccg gcgctgatcc
     2221 gcgacaccgg caagtgcatc tcgtgccgcc gctgcgtgac cgtgtgcaac gaggtccagg
     2281 aggtcggcgg cctgttcgcc cagggccgcg gcttcgccac cctgatcggc ccggcgttct
     2341 cgcagaacct cagcgatgtg gtctgcgtgc agtgcggcca gtgcgccgcc gtgtgccccg
     2401 tgggcgccat ccaggaacgc aaccagatcg acgaggtctg ggcggcgctc gaggatccca
     2461 ccaagaccgt catcgtgcag acggcgccgg cgatccgcgc cgcgctgggc gagtgcttcg
     2521 actacccggc cggcaccctg gtgaccggca agatggtcgc cgccctccgc cgcctcggct
     2581 tcgacgccgt cttcgacacc gacttcaccg ccgacctcac catcatggaa gagggcacgg
     2641 agctgctcgg gcgcctcaag aaggcgctcg tcgacggcga agaggtcgct ctgccgcagt
     2701 tctccagctg ctcgccgggc tggatcaact acatggagca cttcaacccg gacatgctcg
     2761 ccaacctgag cacctgcaag agcccgcagc agatgttcgg cgcgctcgcc aagacgtact
     2821 acgccgagaa gatcggcgtg cagcccgagg acatggtcgt cgtctcggtg atgccgtgca
     2881 cggcgaagaa g
//
LOCUS       DASUMX010000059         2627 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1597722, whole genome shotgun sequence.
ACCESSION   DASUMX010000059 DASUMX010000000
VERSION     DASUMX010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1597722"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            65..>2627
                     /gene="ileS"
                     /locus_tag="VFZ86_02355"
     CDS             65..>2627
                     /gene="ileS"
                     /locus_tag="VFZ86_02355"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEX5641162.1"
                     /translation="MREDQPRFQDVSARVDFPALDARILKFWKDHGIFEKSLEGRGDA
                     PLYVFYEGPPTANGKPGSHHVVARIFKDLFPRYKTMCGYRVPRKAGWDTHGLPVELEV
                     ERRLGIDGKGQIEAYGVAEFNQLCKESVTAYLEEWERFTERIGFWVDMDDAYYTFTND
                     YIESVWWILRQIWDKDLLYQGHKVVPYCPRCGTAISSHEVAQGYKDVTEDSIYVRFPL
                     TAESAERVSGAAAGGGDAGAVGAAGAAGAAGADGEPKRVSLAVWTTTPWTLISNVAAA
                     VRADVTYALVESRGERFVLARDLVDKVLGKKAEVVREFPGAELLGLDYEAPFGFMTSD
                     KRAHFVIGDDYVTTTDGTGIVHIAPAFGEADMRVGVENDLPVVNAVDTEGKFVAEVTP
                     WAGVFVKDADPAITADLKARGLLLGVEPYAHSYPFCWRCDTALLYYAKATWYVRTTAI
                     KDQLIKSNDDVIWHPEHIKTGRFGEWLAGNVDWALSRDRYWGTPLPIWRCEQGHTHCV
                     GSIAELKEMAAGPVPEDLELHRPYVDDVVLTCPDCAGEMRRVPEVIDAWFDSGSMPFG
                     QWHYPFENQELFRERFPADYICEAIDQTRGWFYSLLAVATLVEGRSSFKRVVCLGHIL
                     DADGQKMSKSKGNIVQPDDILDHQGADAFRWFMFASQQPWSPRRFSADMVDEVVRKFL
                     LTLWNTYSFFTVYANIDRFDPAVEDVPVEQRPLLDRWLIGELNTLVQDATAGLEAYDA
                     TGTCRAIQQFVDDLSNWYVRRSRRRFWKSESDADKLAAYHTLYEALVTVTKLLAPFTP
                     FVAEELYQNLVRSVDGPAAGAATSAAAAPAPESVHLSSWPVADEQLIDAGVSFDMA"
BASE COUNT          433 a          944 c          869 g          381 t
ORIGIN      
        1 tcgggccccg gcgtgcgtct ggtagaatcg ggcgcccggc ccagaccagc agaaagcgag
       61 tcacgtgaga gaagatcagc cccggttcca ggacgtctcg gcgcgcgtcg acttcccggc
      121 gctggacgcg cgcatcctta agttctggaa ggaccacggc atcttcgaga agagcctcga
      181 gggccgcggg gacgcgcccc tctacgtctt ctacgagggt cccccgacgg ccaacggcaa
      241 gcccggctcg caccacgtcg tcgcgcgcat cttcaaggac ctcttcccgc gctacaagac
      301 gatgtgcggc taccgcgtgc cgcgcaaggc cggctgggac acgcacggtc tcccggtgga
      361 gctcgaggtc gagcgccgcc tcggcatcga cggcaagggg cagatcgagg cctacggcgt
      421 cgccgagttc aaccagctgt gcaaggagag cgtcaccgcc tacctcgagg agtgggagcg
      481 cttcaccgag cgcatcggct tctgggtgga catggacgac gcgtactaca ccttcaccaa
      541 cgactacatc gagtcggtgt ggtggatcct gcggcagatc tgggacaagg acctgctgta
      601 tcagggccac aaggtcgtcc cgtactgccc gcgctgcggc accgcgatca gcagccacga
      661 ggtggcgcag ggctacaagg acgtcaccga ggattccatc tacgtgcgct tcccgctcac
      721 ggcggagtcg gctgagcggg tgagcggcgc ggcggccggt ggcggcgacg ccggcgcggt
      781 cggcgcggcc ggagcggccg gagcggccgg agcggacggc gagcccaagc gcgtctcgct
      841 cgccgtctgg accaccacgc cgtggacgct catcagcaac gtcgcggccg cggtgcgtgc
      901 ggatgtgacc tacgcgctcg tggagagccg cggcgagcgc ttcgtgctcg cccgcgacct
      961 cgtggacaag gtcctcggca agaaggccga ggtcgtgcgc gagttcccgg gcgccgagct
     1021 gctcggcctg gactacgagg cgccgttcgg cttcatgacc tcggacaagc gtgctcactt
     1081 cgtcatcggc gacgactacg tcacgaccac ggacggcacc ggcatcgtgc acatcgcgcc
     1141 ggccttcggc gaggccgaca tgcgcgtcgg cgtggagaac gacctgccgg tggtcaacgc
     1201 cgtcgacacc gagggcaagt tcgtcgccga ggtcacgccg tgggccggcg tcttcgtcaa
     1261 ggacgcggac ccggccatca ccgccgacct caaggccaga ggccttctcc tcggcgtcga
     1321 gccctacgcg cacagctacc ccttctgctg gcgctgcgac acggctctgc tctactacgc
     1381 caaggcgacc tggtacgtcc gcaccacggc gatcaaggac cagctcatca agtccaacga
     1441 cgacgtgatc tggcacccgg agcacatcaa gaccgggcgc ttcggcgagt ggctcgccgg
     1501 caacgtcgac tgggcgctca gccgcgaccg ctactggggc acgcccctgc ccatctggcg
     1561 ctgcgagcag gggcacacgc actgcgtcgg gtcgatcgcg gagctcaaag agatggcggc
     1621 cggacccgtt ccagaggatc tggaactcca ccggccctac gtcgacgacg tcgtcctcac
     1681 ctgccccgac tgcgccggcg agatgcgccg cgtcccggag gtcatcgacg cctggttcga
     1741 ctccggctcc atgccgttcg gccagtggca ctacccgttc gagaaccagg agctcttccg
     1801 cgagcgcttc cccgccgact acatctgcga ggccatcgac cagacgcgcg gctggttcta
     1861 cagcctgctc gccgtcgcca cgctcgtcga gggccgcagc tccttcaagc gcgtcgtctg
     1921 cctcggccac atcctcgacg ccgacggcca gaagatgagc aagagcaagg gcaacatcgt
     1981 gcagcccgac gacatcctcg accaccaggg cgccgacgcc ttccgctggt tcatgttcgc
     2041 gagccagcag ccctggagcc cccgccgctt cagcgccgac atggtcgacg aagtggtgcg
     2101 caagttcctg ctcaccctgt ggaacacgta cagcttcttc accgtctacg ccaacatcga
     2161 ccgcttcgac ccggcggtgg aggacgtgcc ggtggagcag cggccgctgc tcgaccgctg
     2221 gctgatcggc gagctgaaca cgctcgtcca ggacgccacc gccggcctcg aggcctacga
     2281 cgccaccggc acctgccgcg cgatccagca gttcgtcgac gacctcagca actggtacgt
     2341 gcgccgcagc cggcgtcgct tctggaagag cgagagcgac gccgacaagc tggccgccta
     2401 ccacacgctc tacgaggcgc tcgtcacggt gaccaagctg ctcgcgccgt tcacgccgtt
     2461 cgtcgccgag gagctgtacc agaacctggt gcgctcggtg gacgggccgg cggccggcgc
     2521 ggccacgtca gcggccgccg cccccgcgcc cgagagcgtg cacctgagct cctggccggt
     2581 cgccgacgag cagctgatcg acgcgggcgt ctcgttcgac atggcgg
//
LOCUS       DASUMX010000060         9303 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_592168, whole genome shotgun sequence.
ACCESSION   DASUMX010000060 DASUMX010000000
VERSION     DASUMX010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 9303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9303
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592168"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            236..598
                     /locus_tag="VFZ86_02360"
     CDS             236..598
                     /locus_tag="VFZ86_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641163.1"
                     /translation="MSHTIAHRPHVHHLPLAPVVALIAIVLIAAVVIWAVGQPAPTTT
                     TTTVGASIVSPVVHPAAVAAPESPVFRHALMRVNGGDGYPLASIVGRYHMVEGATLDP
                     VSTNPYGTASYKAPHYPR"
     gene            642..1418
                     /locus_tag="VFZ86_02365"
     CDS             642..1418
                     /locus_tag="VFZ86_02365"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEX5641164.1"
                     /translation="MPIYADIGISTGAYANFSLAAALLRIGEIAPSAEILSLGRHSLL
                     DAANARVVDLAGLPFTVHGPFLHFEYGSRSGRQHRRALDEHRRQLEVAGALGALVYVV
                     HPDLQRRYKGWDTRVVATLESSFEALALLQEEYGVTIAVENLPFVKHTHFRAPGDLDL
                     RGLGLSLDVGHAAVTGTLSAWLADDRYLLKHVHLHDNGGHVGGDQHLPLGRGVIDAAP
                     VMELARAAGAMVVLEHKNEADVIESLRHLHERGLLERAIR"
     gene            1429..3273
                     /locus_tag="VFZ86_02370"
     CDS             1429..3273
                     /locus_tag="VFZ86_02370"
                     /inference="COORDINATES: protein motif:HMM:NF015410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF294 nucleotidyltransferase-like
                     domain-containing protein"
                     /protein_id="HEX5641165.1"
                     /translation="MEITPGADPARIADRVRFLSRVRPFKDLPPEELRDVAAAVRERA
                     VPAGEAILVEGGPPGEELYVVRLGALELVHKDAYVAVLAAGEVVGHPTLLTGLAPEFT
                     TRAREDSMLYCLPREVAFEVLSRPSGLQWMAANARERLLQAAGTMRALPDVRVMPVTS
                     VTRSKPLFCDPETTAREAAKIMIAEKRSAIIVRGPGGRGIAGIVTDVDLRDKVVVGGV
                     SRHAPVTEIMSAPVHTIGAQVLAPEASIAMMTAGVNHLPVLDDEGEVVGVLSASNLMS
                     LEARSPFALRREIMASRTEDEVVTAAADIPQLFLDLMTANIDAPSMTRVFTVLHDAIT
                     SQLLELVIDRLGEPPVPYAWLAFGSAARSELTLASDQDNGLAYDDTDDPAVADYFRVL
                     ARDVNTGIARCGLPADPHGVLASNGAWRMPLSGWRSTFEDCLEGKDLDRMARASVSFD
                     YRQVAGDLYVDKVLTDVMREAPEHRRFLEGLIQLGTRVRPPLGFRGKLEGSIDIKKNG
                     LVPIQNYARYFAFASSITSASTLERLGAIVEVGGDDVIPERALREAFISMQHLQLRHH
                     ANRLRHGRPVDNIIETWTLPPLTRASLQEALREIVSAQKRFRPVEAPR"
     gene            3372..3752
                     /locus_tag="VFZ86_02375"
     CDS             3372..3752
                     /locus_tag="VFZ86_02375"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5641166.1"
                     /translation="MKLLIAHGDAHERDVLREMARDLLRLGLEPVESGEGAHAMSLLL
                     AANAPALAVVDWDLPGCGGPELCRRLRSQRRSRPPYIILLTPSAEQAGEGFEAGADDC
                     IQAAAGGHELQARIVAARRLAALG"
     gene            3787..5040
                     /locus_tag="VFZ86_02380"
     CDS             3787..5040
                     /locus_tag="VFZ86_02380"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX5641167.1"
                     /translation="MPRTRPRIPPDDDLSWAGDEPRPPAHRWVFATTVAFLGVAAVLR
                     SLLAFEGEQRILVLALLAVWFVLVLAEPFAARAWPPSFVAYLTAQGAVVLLLLTQSDS
                     SDFFAVLLAVPVMQAAERWQPRAVWVLIAVFAGLTGVGLIEEYGGSSTVSLVAIYAAA
                     NVFFAAYALAARRAVEVRTRNEALAADLRQANQRLTESSERAQRLGAARERQRLARDL
                     HDSVTQTLFSMTLTARSALLLLQREPREVPAQLDQIDHLAHSALEEMTALAAEPPASA
                     PGGGRLAGALGRHCTERKLRDGLAVSLEVDGDEPLPAKQEAALLRIAQEGLNNVVKHA
                     GVEEAAVRLRLRRPYRLEVEDDGSGFDPAERAGSGLGLTGMAERAAEIGWRLTVTSRP
                     GGGTRVVAEAGETGETDGEGGTDGD"
     gene            5033..5743
                     /locus_tag="VFZ86_02385"
     CDS             5033..5743
                     /locus_tag="VFZ86_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019358253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5641168.1"
                     /translation="MTESAPIRVLIVDDHQVVRRGLRTFLELHDDLDVVGEAGDGLEA
                     VEQAAALRPDVVLMDIVMPRLDGIEATRRIKEQSAAVGVIALTSFAGDDQVLPALEAG
                     ASSYLLKDVSPEELVEAIRAVHRGEPRLHPDALRKVMEAAMGPRADEAPARAPSPPGD
                     LTERELEVVALVAQGLSNREIAETFVLSEKTVKTHIGHILAKLELKDRTQLAIYALKH
                     GLAGDAEGRGAPGGPRKE"
     gene            5751..6536
                     /locus_tag="VFZ86_02390"
     CDS             5751..6536
                     /locus_tag="VFZ86_02390"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX5641169.1"
                     /translation="MAAPVQPASLPNRLGVIAMVVVWAAVLARTLSFAGAGEWPGYLA
                     GFAAFLVILLVVVLRRPVSDPLVHLAFVVQVAIVLVLLTVDPQRDFVTVLFVLMCYEA
                     AVAFPALPRTIWVAALVALMGGSLVLELGLVRGLALGLIPMAAGVVLSTYVVVTRELG
                     AERAASERMVADLRAAHERLQAYSGQADELAAIEQRARVARDLEQSVTRTLGSALEAS
                     AAARELLSEPAQAGPQLERLQELTTQALAQMRRVITELRPSQG"
     gene            6725..7918
                     /locus_tag="VFZ86_02395"
     CDS             6725..7918
                     /locus_tag="VFZ86_02395"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEX5641170.1"
                     /translation="MNALDATTDRLHFIDWLRVLAVLLLFPYHTLRVFNTEAFYVKAS
                     QLSTTLDYALGFISVWHMQLLFLLAGASTYFALRKRSSRQYVGERFTRLGVPFVAGVF
                     LLLIPIQTWYGARFNSGYQDSFWHYLISGDFLRWNIQEGGDYFGGFGFGHLWFLFVLL
                     FVALIAAPLLANRGRGGALLQGLSRRLAHPAWWLVAALLIMLGAAVPDPTEALEPFYY
                     LVFFVLGYVAVCAPGFLQSAERYRLPALAAGVALAVWWVLSGSLRDSLPDPSLQIAGL
                     SFLGALASWLVIVGLLGYGRRYLNRSSAGLAYLAESSYPVYLLHQSVIVVAAFYLVGL
                     PAAEPLQWLTLLVVSVAATFALYELVRRFQTTRFLFGMRPLRRTGATAPRSVPSRDGE
                     GAAAS"
     gene            complement(7933..8793)
                     /locus_tag="VFZ86_02400"
     CDS             complement(7933..8793)
                     /locus_tag="VFZ86_02400"
                     /inference="COORDINATES: protein motif:HMM:NF018506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX5641171.1"
                     /translation="MLASLLALVASLSWGTADFWAGLESRRTTVWTAALIGQGVAAVA
                     LVLMLLVLAPDTPSAAALVPAVLGGVAGGVGALLQYRALTVLDMSVASPIVAGAALVP
                     VLWGLARGEQPGALQIAGVAMTLAGIVAISRGPSRDAEGERVGPPGRADRAGVLLAIG
                     SAVTLGLFLVAVDYGDEAGTLWTVTVVRAVAFVVLALMAAVSGPVLRPRGRALPILVA
                     VGLLIATANISFASATSMGYLSIVAVLGWLNPAVTIVWARAVLHERLRPLQVAAAVLV
                     FAGIVCITLG"
     gene            8892..>9303
                     /locus_tag="VFZ86_02405"
     CDS             8892..>9303
                     /locus_tag="VFZ86_02405"
                     /inference="COORDINATES: protein motif:HMM:NF014839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HEX5641172.1"
                     /translation="MTLHVTLPDGSSLELDDGASVRDAAAAIGPRLAKAAIAGRVTAP
                     DEPDVTLLVDATAPLHDGDKLDIVTLKGDDPDAVDIQRHTASHVMAQAIVHLFPGARY
                     AIGPTIENGFYYDLQLPEPIAEADLARIEKEMGKV"
BASE COUNT         1278 a         3462 c         3241 g         1322 t
ORIGIN      
        1 cgcctccgcg ccctcggtcg tcgggtccat cgtctcccct ctgattccgt cctctgcggc
       61 cagcatcggc accagcacgc ctcgtcgtga gtcacggcgc ctgcctgaga cgcggcgtca
      121 cggtccttcg cttgcagtgc ggttcacccc ttttgggcga tgcacgacga gtgcccccgc
      181 gcctacgctg gacccgtggt cggagaacca catgtccgcg agaggggagc gcgtcatgag
      241 tcacaccatc gcccacaggc cccatgtcca tcaccttccg ttggcgccgg tcgtcgcgct
      301 gatcgccatc gtgctgatcg ccgccgtggt gatctgggcc gtcggtcagc cggcgccgac
      361 gacgaccacc acgaccgtgg gcgcgagcat cgtctcgccc gtggtccatc cggcggcggt
      421 cgccgctccg gaaagccccg tgttccgcca cgcgctgatg cgcgtcaacg gcggagacgg
      481 ctacccgctc gcctccatcg tcggccggta ccatatggtc gagggggcga cgctcgaccc
      541 ggtcagcacc aacccgtacg gcacggcgtc gtacaaggcg ccccactacc cccgctgaca
      601 ggcgagccat gaggcacccg gcgtgcgata ctgcataccg ggtgcccatc tacgcagaca
      661 tcgggatctc gacgggggcg tacgcgaact tctcgctcgc ggcggctctg ctgcgcatcg
      721 gcgagatcgc gccctcggcc gagatcctct cgctgggccg gcacagcctg ctcgacgccg
      781 ccaacgcgcg cgtcgtcgat ctcgccggtc tgccgttcac cgtccacggg ccgttcctcc
      841 acttcgagta cggcagccgc tcgggacggc agcaccggag ggcgctcgac gagcaccgcc
      901 ggcagctcga ggtcgcgggc gcgctcgggg cgctcgtcta cgtggtgcac cccgacctcc
      961 agcgccggta caaggggtgg gacacgcgcg tcgtcgccac gctcgagagc tccttcgagg
     1021 cgctcgcgct gctgcaggag gagtacgggg tgacgatcgc cgtcgaaaac ctgcccttcg
     1081 tcaagcacac ccacttccgg gccccaggcg atctcgacct ccgcggcctc ggactctcgc
     1141 tggacgtcgg acacgcggcc gtcaccggca ccttgtccgc gtggctcgcc gacgaccgct
     1201 acctgctcaa gcacgtgcac ctgcacgaca acgggggcca cgtgggcggc gaccagcatc
     1261 tcccgctggg gcgcggcgtc atcgacgccg cgcccgtcat ggagctggcc cgcgccgccg
     1321 gagccatggt cgtgctcgag cacaagaacg aggccgacgt gatcgagagc ctgcgccacc
     1381 tgcacgagcg cgggctcctc gaacgcgcga tcaggtagag gagcagagat ggagatcacg
     1441 ccgggcgccg atcccgcacg tatcgccgac agggtccgct tcctctcgcg ggtccgcccg
     1501 ttcaaggacc tgccgcccga ggaactgcgg gacgtcgccg ccgcggtgcg cgagcgcgcc
     1561 gtgcctgcgg gcgaggcgat cctcgtggaa ggcgggccgc ccggcgagga actctacgtg
     1621 gtgcgactcg gcgcgctcga gctggtccac aaagatgcct acgtcgccgt cctcgcggcc
     1681 ggtgaggtcg tcggtcaccc gacgctgctc accggcctcg cccccgagtt cacgacgagg
     1741 gcgcgggagg actcgatgct ctactgcctg ccgcgcgaag tcgcgttcga ggtgctcagc
     1801 cgcccgtccg gcctccagtg gatggccgcc aacgcccgcg agcgtctgct gcaggcggcc
     1861 gggacgatgc gcgccctgcc cgacgtgcgc gtcatgccgg tcacgtcggt gacgcgcagc
     1921 aagccgctgt tctgcgaccc ggagacgacg gcgcgggagg ccgcaaagat catgatcgcc
     1981 gagaagcgct cggcgatcat cgtgcgcggc cccggcggca gaggcatcgc cggcatcgtc
     2041 accgatgtcg acctgcgcga caaggtggtc gtcgggggcg tgtcgcgtca cgcccccgtc
     2101 acggagatca tgtccgcacc ggtgcacacg atcggcgcgc aggtgctcgc cccggaggcg
     2161 agcatcgcga tgatgaccgc cggcgtgaac cacctcccgg tgctcgacga cgagggggag
     2221 gtggtgggcg tcctgtccgc cagcaacctg atgtcgctcg aggcgcgcag cccgttcgcc
     2281 ctgcgtcgcg agatcatggc gtcgcgcacc gaggacgagg tcgtcacggc ggcggcggac
     2341 atcccccagc tcttcctcga cctcatgacc gcgaacatcg acgccccgtc catgacccgg
     2401 gtgttcacgg tgctccacga cgcgatcacc tcgcagctgc tcgagctggt catcgaccgg
     2461 ctgggcgagc cgcctgtgcc ctacgcctgg ctcgcgttcg gcagcgccgc gcggtccgag
     2521 ctcacgctcg cttccgacca ggacaacggg ctggcctacg acgacaccga cgaccccgcc
     2581 gtggccgact acttccgcgt cctggcgcgg gacgtcaaca cgggcatcgc ccgctgcggc
     2641 ctgcctgccg acccgcacgg cgtgctcgcc agcaacggcg cctggcggat gccgctctcc
     2701 gggtggcggt ccaccttcga agactgtctc gagggcaagg acctggatcg catggcccgc
     2761 gccagtgtct cgttcgacta ccgccaggtc gccggcgacc tctacgtcga caaggtgctc
     2821 accgacgtga tgcgcgaggc cccggagcac cgccggttcc tcgaggggct gatccagctg
     2881 ggcacgcggg tacgaccgcc gctcggcttc cgcggcaagc tcgagggcag catcgacatc
     2941 aagaagaacg ggctcgtgcc catccagaac tacgcgcgct acttcgcctt cgcctcgtcc
     3001 atcacgtcgg cgtccaccct cgagcggctc ggcgcgatcg tcgaggtcgg cggcgacgac
     3061 gtgatccccg agcgcgccct ccgcgaggcg ttcatcagca tgcagcacct gcagttgcgc
     3121 caccacgcca accgcctccg ccacgggcgt cccgtcgaca acatcatcga gacgtggacg
     3181 ctgccgccgc tcacgcgcgc gagcctccag gaagctctgc gcgagatcgt ctccgcacag
     3241 aagcggttca ggccggtgga ggcgccgcgc taggcgatcg ttctatcccc agcggaggcc
     3301 cgaggcccga gaatgggagc gagagagtac gccgcgccat ccggcgcggc ggtccgcccc
     3361 ggggggaacg tgtgaagctg ctcatcgctc acggcgatgc ccatgagagg gacgtcctgc
     3421 gcgagatggc cagggacctc ctccggctcg ggctcgagcc ggtcgagtcc ggcgagggcg
     3481 cccacgccat gtccctgctc ctggcggcca acgctcccgc tctcgccgtg gtcgactggg
     3541 acctgccggg ctgcggcggg ccggagctgt gccggcgtct gcgctcgcag cggcgctccc
     3601 ggccccccta catcatcctc ctcaccccca gcgcggaaca ggccggcgag ggcttcgagg
     3661 ccggcgccga cgactgcatc caggctgccg ccggggggca cgagctgcag gcgcgcatcg
     3721 tcgccgcgcg ccggctggcc gccctgggct gaccggtcct cggcgtggcg ggcgtaggat
     3781 ggccccatgc ccaggacgag accgaggata cccccggacg acgacctctc atgggccggc
     3841 gacgagccgc gcccgccggc ccaccgctgg gtgttcgcga ccaccgtcgc gttcctgggg
     3901 gtcgcggccg tgctgcgctc gctgctcgcc ttcgagggcg agcagcggat cctcgtcctc
     3961 gccctgctcg ccgtctggtt cgtgctcgtg ctcgccgagc cgttcgcagc gcgcgcctgg
     4021 ccgccgtcgt tcgtcgctta cctgaccgcg cagggcgcgg tcgtcttgct gctgctgacg
     4081 caatccgact cgagcgactt cttcgccgtg ctgctggcgg tccccgtgat gcaggcggcc
     4141 gagcgctggc agccgcgcgc cgtgtgggtg ctcatcgccg tgttcgcagg gctgaccggc
     4201 gtcggcctca tcgaggagta cggtggctcc tcgaccgtct cgctggtggc gatctacgcc
     4261 gccgcgaacg tgttcttcgc ggcctatgcg ctggccgccc ggcgcgccgt cgaggtgcgg
     4321 acgcgcaacg aggcgctggc cgccgacctg cggcaggcca accagcgcct gaccgaatcc
     4381 tccgagcggg cccagcggct gggcgccgcg cgcgagcgcc agcggctggc gcgcgacctg
     4441 cacgattcgg tgacgcagac cttgttcagc atgaccctca ccgcccggtc ggcgctcctg
     4501 ctcctgcagc gtgagccccg cgaggtgccg gcgcagctcg accagatcga ccatctcgcg
     4561 cacagcgccc tggaggagat gaccgcgctg gccgccgagc caccggcctc cgctcccgga
     4621 ggaggccggc tcgccggcgc cctcgggcgg cactgcacgg agcgcaagct ccgggacggg
     4681 ctggcggtga gtctcgaggt ggacggcgac gagccgctgc cggcgaagca ggaggcggcc
     4741 ctcctgcgca tcgcccagga gggcctgaac aacgtggtca aacatgcggg ggtggaggag
     4801 gcggcggtgc gcctgcgcct gcgccgcccc taccgcctcg aggtggagga cgacggcagc
     4861 ggcttcgacc cggccgagcg cgccggctcc gggctgggcc tcaccggcat ggcggagcgc
     4921 gccgccgaga tagggtggcg cctgacggtg accagccgtc cgggcggcgg gacgcgggtc
     4981 gtggcggagg ccggcgagac cggcgagact gacggcgagg gaggaaccga cggtgactga
     5041 gagcgcaccg atcagggtgc tgatcgtgga cgaccatcag gtggtccgcc ggggtctgcg
     5101 gaccttcctc gagctgcacg acgacctcga cgtggtcggc gaggcgggcg acgggctcga
     5161 ggcagtggag caggccgccg cgcttcggcc ggacgtggtc ctcatggaca tcgtcatgcc
     5221 gcgtctggac ggcatcgagg ccacgcggcg catcaaggag cagtcggccg ccgtgggggt
     5281 gatcgccctg accagcttcg ccggggacga ccaggtgctg ccggccctgg aggcgggcgc
     5341 ctccagctac ctgctcaagg acgtgagccc ggaggaactg gtggaggcga tccgcgccgt
     5401 gcaccggggc gagccgaggc tgcaccccga cgcgctgcgc aaggtgatgg aggcggcgat
     5461 ggggccgcgg gcggacgaag cccccgcccg cgcgccgtcg ccgcccggcg acctcaccga
     5521 gcgcgagctc gaggtcgtcg ccctggtcgc gcagggcctc agcaaccgcg agatcgcgga
     5581 gaccttcgtc ctcagcgaga agacggtgaa gacgcacatc ggccacatcc tcgccaagct
     5641 ggagctcaag gacaggacgc agctggccat ctacgcgctc aagcacgggc tcgccggcga
     5701 cgccgagggg cggggcgccc ccggcggacc gcggaaggag taggggggcc gtggccgcgc
     5761 ccgtgcagcc ggcgtcactg ccgaaccgcc tcggcgtcat cgccatggtg gtggtgtggg
     5821 cggcggtcct ggcgcgcacc ctgagtttcg ccggcgccgg agagtggccc gggtatctcg
     5881 ccggcttcgc cgccttcctg gtcatcctgc tggtcgtcgt gctgcgacgc cccgtctccg
     5941 acccgctggt gcatttggcc ttcgtcgtcc aggtcgccat cgtcctggtg ctgctgacgg
     6001 tcgacccgca gcgcgacttc gtcaccgtgc tgttcgtcct gatgtgctac gaggccgcgg
     6061 tcgcgttccc ggccttgccg cgcaccatct gggtggcggc gctcgtcgcg ctgatgggcg
     6121 ggtcgctggt gctggagctc gggctcgtcc gcggcctcgc gctcggactg atccccatgg
     6181 cggcgggcgt cgtgctctcg acctacgtcg tcgtgacccg cgagctcggc gccgagcggg
     6241 cggccagcga gcgcatggtc gccgacctgc gggcggcgca cgagcgcctg caggcctaca
     6301 gcggccaggc cgacgagctg gccgcgatcg agcagcgcgc ccgggtcgcg cgcgacctgg
     6361 agcagtcggt cacgcgtacg ctggggagcg cgctcgaggc cagcgccgcg gcgcgggagc
     6421 tcctgtcgga gccggcgcag gccggccccc agctcgagcg cctccaggag ctgaccacgc
     6481 aggcgctcgc ccagatgcgc cgcgtcatca ccgagctgcg cccgtctcaa ggctgaggcc
     6541 ggccggaccg ccctccccat ccggccaggc tcaccctcgg acctgggtcg cacctcctcc
     6601 accccaggtc ccacgcgaaa gatacgccgc tcgtcgcccg ccggatgctc ctccgggtcg
     6661 atgtgcagga gctcgcccgg cgagatgatc gggacgtcga tcgcagagac cacggggagg
     6721 aaccatgaac gccctcgacg ccaccactga ccggctccac ttcatcgact ggctgcgggt
     6781 gctggccgtg ctgctgctct tcccgtacca cacgctgcgg gtcttcaaca ccgaggcctt
     6841 ctacgtgaag gcgagccagc tgtcgacgac cctcgactac gcgctcggct tcatctcggt
     6901 gtggcacatg cagctgctct tcctgctggc cggcgcctcc acgtacttcg cgctgcgcaa
     6961 gcgcagctcg cggcagtacg tgggcgagcg cttcacccgg ctcggcgtcc cgttcgtcgc
     7021 cggcgtgttc ctcctgctga tcccgatcca gacctggtac ggcgcccgct tcaactccgg
     7081 ctaccaggat tcgttctggc actacctgat cagcggtgac ttcctccgct ggaacatcca
     7141 ggaaggcggc gactacttcg gcggtttcgg cttcggccac ctgtggttcc tcttcgtgct
     7201 gctcttcgtc gccctgatcg ccgcaccgct gctcgcgaac cgcgggcgcg gcggcgcgct
     7261 gctgcagggc ctttcgcgcc gcctcgcgca ccccgcctgg tggctggtcg cggcgctgct
     7321 catcatgctc ggcgcggcgg tgccggaccc gaccgaggcg ctggagccct tctactacct
     7381 cgtgttcttc gtgctcggct acgtcgcggt ctgcgcgccg ggcttcctgc agtcggccga
     7441 gcggtaccgc ctgccggcgc tggccgccgg tgtcgccctc gccgtctggt gggtgctcag
     7501 cggcagtctg cgcgattcgc tgccggatcc ctcgctgcag atcgcgggcc tgagcttcct
     7561 cggcgcgctc gcctcctggc tggtcatcgt cggcctcctg ggctacggcc gccggtacct
     7621 caatagaagc tctgcggggc tcgcctacct ggcggagtcg tcgtacccgg tctacctgct
     7681 ccaccagtcg gtgatcgtgg tcgccgcctt ctacctggtc ggcctgcccg ccgccgagcc
     7741 gctccagtgg ctcacgttgc tggtcgtctc cgtggccgcg acgttcgccc tgtacgagct
     7801 ggtgcgccgg ttccagacca cgcgcttcct cttcggcatg cgcccgctca gaaggaccgg
     7861 cgccacggcg ccgcggagcg tcccgagccg cgacggcgag ggcgcggcgg cctcgtgaca
     7921 ccggagtcgc cgctacccga gggtgatgca gacgatcccc gcgaacacga gcacggccgc
     7981 cgcgacctgc aacggccgca ggcgttcgtg caggactgcc cgtgcccaca cgatggtgac
     8041 ggccgggttc agccagccga ggaccgcgac gatgctgagg tagcccatcg acgtcgccga
     8101 cgcgaagctg atgttggcgg tcgcgatcag gagccccacc gcgaccagga tcggcagcgc
     8161 ccggccgcga ggccgcagca ccggccctga gacggcggcc atgagggcga gcacgacgaa
     8221 cgccaccgcg cgcacgacgg tgaccgtcca gagggtcccc gcctcgtccc cgtagtccac
     8281 cgcgacgagg aacaggccca gcgtgacggc agagccgatc gccaggagga ccccggcgcg
     8341 gtcggcgcgc ccaggtgggc cgacgcgttc accctctgcg tcgcgcgaag gcccgcgcga
     8401 gatcgcgacg atccctgcca gggtcatcgc gacgccggcg atctgcaggg ccccgggctg
     8461 ctccccgcgc gccagtcccc acagcaccgg gaccagcgcg gcgccggcga cgatgggcga
     8521 ggcgacgctc atgtcgagca cggtgagggc gcggtactgc agcagcgcgc cgactccgcc
     8581 ggcgacgccg ccgagcacgg ccgggacgag cgccgcggcc gaaggggtat ccggcgcgag
     8641 gacgagcagc atcaggacga gagcgacggc cgcgacgccc tgcccgatga gcgcggccgt
     8701 ccagacggtc gtcctgcgcg actccaggcc ggcccagaag tcggcggtgc cccaggagag
     8761 gctggcgacc agagcgagaa gagaagctag cacggcgcgc ggatgctacc acggctccgt
     8821 ctgctagact cggccggtca acagcccccg caaggtgcgc gggcgcgcaa gcccagaaag
     8881 gaccaccccg catgaccctg cacgtcaccc tccccgacgg ttcctccctc gagctcgacg
     8941 acggcgccag cgtgcgcgat gccgcggcgg cgatcgggcc gcgactggcg aaggccgcca
     9001 tcgcgggtcg cgtcaccgct cccgacgagc cggacgtcac cctgctcgtc gacgccaccg
     9061 cgcccctgca cgacggcgac aagctcgaca tcgtcacgct caaaggtgac gatcccgacg
     9121 ccgtcgacat ccagcgccac accgcctcac acgtcatggc gcaggccatc gtccatctgt
     9181 tcccgggcgc caggtacgcc atcggcccga ccatcgagaa cggcttctac tacgacctgc
     9241 agctgccgga gccgatcgcg gaggccgacc tggctcgcat cgagaaggag atgggcaagg
     9301 tcg
//
LOCUS       DASUMX010000061         7595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_2030097, whole genome shotgun sequence.
ACCESSION   DASUMX010000061 DASUMX010000000
VERSION     DASUMX010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 7595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7595
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2030097"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="VFZ86_02410"
     CDS             complement(<1..374)
                     /locus_tag="VFZ86_02410"
                     /inference="COORDINATES: protein motif:HMM:NF014173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX5641173.1"
                     /translation="MRLELTKRGDYAVRAMIALARDDAGGLMSARTIAAAMAIPPRFL
                     PQVMADLVRAGLVEGVTGRSGGYRLSVAAADTSLLRIVEAVEGSSRRETCVLRGGPCG
                     RDGACAVHAAFCSAEEAMIGAL"
     gene            complement(451..1740)
                     /gene="nrfD"
                     /locus_tag="VFZ86_02415"
     CDS             complement(451..1740)
                     /gene="nrfD"
                     /locus_tag="VFZ86_02415"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEX5641174.1"
                     /translation="MSTVVTPAAAPAEGRERRALRLWIGLLALGIAVGLGAWVYQLAN
                     GLAATNMRNPMMWGLYITMFMYFVGLSAGGLIVASAGRLFGAKQLKPVVRIAVLEATV
                     AVMIAALLLLPDIGRPDRAWHLLRYPQLMSPLIWDLTIVTVYFLISATYVWVYTRSDL
                     AARGSRLALGTGTSLADARRDERLKGVLAAVGLPAAILLHSITAWIFGLQIARGFWYS
                     AIMAPLFIASALVSGTALMIVLALIVRRTGKVGFKDDLVSYLGGMLAVFIAVEAFLVF
                     SEMLTAAYPGAGFEADPARRLLTGPYAGFFWFEVVAGLVVPFVLLVVKRLRADPRWVA
                     AASVLAVAGILIHRVNIVLNGLSYATVPFPPGVPIGTAPPEGATAFALSYFYSPALIE
                     YLVAGGVICFGALVFTLAALWLPLREGGAARLPDAGS"
     gene            complement(1737..2450)
                     /locus_tag="VFZ86_02420"
     CDS             complement(1737..2450)
                     /locus_tag="VFZ86_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX5641175.1"
                     /translation="MADKVSPVEALPPASRAYGELEQPSDEPRWGMVVDQERCIGCWS
                     CCVICKSENDVPLGMWWNRIDTIGDALDTPREVNGELEMHWLPLACQHCENPACTKVC
                     PVAATYPREDGVVMQDPQRCIGCRFCMVACPYGVRTFNWGEPDRPTGFDTGMVPPRPV
                     GTMEKCTFCVHRLAEGQVPSCVWSCPAQARIFGDLNDPDSRVSRLIRERSGEMLLEER
                     GTEPRVYYLPPRRKRGLDT"
     gene            complement(2443..5154)
                     /locus_tag="VFZ86_02425"
     CDS             complement(2443..5154)
                     /locus_tag="VFZ86_02425"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX5641176.1"
                     /translation="MKATRRQFLVGAGVAAGSAAAAGALGPLASAGRDDVQPSLPVDR
                     VVRTTCSPNCTGSCGQLAFVRDGRVVKVQQAADYPDAAYNPRGCMKGISYHLLIHGPD
                     RIQTPLVRTGERGSGEFREATWDEVLEKIAGELQRIGDEWGWDAVHVFSQVPGSGYIQ
                     KGAAYRACAALGMVHGTSFDFNGDLPMGMPITFGVQNAEHESKDWANSRFILLIGANP
                     LETRIPDVHFIHDAVERGARLVVVDPTFSSTAAKADAWVQIKPGTDAAFGLALCNRIV
                     ARDLADWDFMRTYTDAPLLVRTDTGKRLRADELAAGEARDPGPAAGVGLGDYGRQAPP
                     AGTVPGTPAAQSPAHGVPLPNDRYVAWDTVRGEAAVIGTERLGIPAGVDAALDGEYEV
                     KLADGSRVTVTPGFALVRAELDGWTPERAAEITGVPVETIVRVADAFAAERPAAVIMG
                     GGSNHWFHGDLTGRAFALLASLTGNVGRSGGGFSVYVGQYKVRVDTSPWWALGETKAK
                     IVPSVYFVRGRTETMHETVPYPENGFKALICTFANMFLQAMDVNRLHKTLDGCELVVV
                     VDHQMTDTAKWADIVLPAATWYEKTDLTATPLHPYLQIQQEAIPPVGESRPELWMWQE
                     IVKRIDPALFEEYFDVTAEEAIEAMLAQGEGTEGITLEQLKAGPVRLDVPDPDVAFVR
                     QVEELVPFPPQSLPAPLEATAAFIPTRRAEFYKEEDRFLELGEAVPTYKPPHDDEVHD
                     PAEYPIALLSPHSKWRIHSSYANVAWLNEITGGRAPVLLHPSTAGERGIGEGDLVELR
                     NTRGSVRAWAHLTPAARPGSATLFEGWWSRQLGGGKSVNELTSSEVNPIHEIHYVANM
                     WCPSTGWKDCRCEVTRIGDGIDAEIAAAAAPDVAAAPAPSVQEVTHG"
     gene            complement(5151..5423)
                     /locus_tag="VFZ86_02430"
     CDS             complement(5151..5423)
                     /locus_tag="VFZ86_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641177.1"
                     /translation="MTRRRFVVAAGVSALGALAVRAAERLEPAVAAAAPALRPAARGT
                     SAGRCALCGSATHTTLDATCAEAAARRREVQTAARRIGARAGRTDA"
     gene            5699..5908
                     /gene="tatA"
                     /locus_tag="VFZ86_02435"
     CDS             5699..5908
                     /gene="tatA"
                     /locus_tag="VFZ86_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011273528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEX5641178.1"
                     /translation="MPNLGAPELIIIALVILLLFGATRLPKLGKSMGQSIKGFKQGLN
                     DDADDDEIVDVKRDATAEDKDVKAS"
     gene            complement(6005..6460)
                     /gene="ispF"
                     /locus_tag="VFZ86_02440"
                     /pseudo
     CDS             complement(6005..6460)
                     /gene="ispF"
                     /locus_tag="VFZ86_02440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00151.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
     gene            complement(6471..7193)
                     /gene="ispD"
                     /locus_tag="VFZ86_02445"
     CDS             complement(6471..7193)
                     /gene="ispD"
                     /locus_tag="VFZ86_02445"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEX5641179.1"
                     /translation="MDVEDQQAGETWVIVAAAGESQRMGLPDGESKQFLLLGGETILS
                     HSVHRLLAMSCVDGIVVVLHPSHTVRFANELMLFDDSKPVLIAEGGRARSDSVRAGLV
                     MVPDSATVIAVHDGARPLFSKHVFEACVTALRETDGAVPAVEVSDTLKRQGEGGAVAG
                     TVDRAGLWAAQTPQVFRSAALRDVYARTDLNGATDDAMLLERGGYSVRLVPSTPANVK
                     ITTPQDIPLAGALLSWEVETDV"
     gene            complement(7204..>7595)
                     /locus_tag="VFZ86_02450"
     CDS             complement(7204..>7595)
                     /locus_tag="VFZ86_02450"
                     /inference="COORDINATES: protein motif:HMM:NF014047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HEX5641180.1"
                     /translation="DGADYPDIEAVDAKLVRLGHDTGAMIVSTDYTLKKVAEIHGVKM
                     LNVNDLANSVKPAVLPGEIIEVKILREGRERDQGVGYLDDGTMIVVEAGSSLVGSRVK
                     AEVTSVLQSPSGKMIFSRVSAPPPAEN"
BASE COUNT         1104 a         2706 c         2705 g         1080 t
ORIGIN      
        1 tcgagcgcgc cgatcatggc ttcctcggcg ctgcagaagg cggcgtggac cgcgcaggcc
       61 ccgtcgcgcc cgcagggacc gccgcgcagg acgcaggtct cgcggcggct gcttccttcg
      121 acggcctcga cgatccgcag caggctcgtg tccgcggcgg ccaccgagag cctgtagccg
      181 ccgctccttc cggtgacgcc ctcgacgaga ccggcgcgca ccaggtcggc catgacctgc
      241 ggcaggaagc gcggcgggat cgccatcgcc gcggcgatcg tcctggcgct catcagcccg
      301 ccggcatcgt cgcgcgccag ggcgatcatc gcgcgcaccg cgtagtcgcc gcgtttggtc
      361 agctcgagcc tcatgacgca agcctatcgt gacgagggcg gcgcgccacg tgccggtccc
      421 ggccacgcgg cggcaagtcg aagcggctgg tcacgacccg gcgtccggca gccgcgccgc
      481 gccaccctcc cgcagcggca gccacagcgc ggcgagcgtg aagacgagcg cgccgaagca
      541 gatcacgccg ccggccacca ggtactcgat gagcgccggg ctgtagaagt agctgagcgc
      601 gaacgcggtc gccccctccg gcggcgcggt gccgatcggc acgcccggcg gaaacggcac
      661 ggtcgcgtac gagaggccgt tgagcacgat gttgacgcgg tggatgagga tgccggccac
      721 cgcgagcacg gaggccgccg ccacccagcg cggatcggcg cgcaaccgtt tgaccaccag
      781 caggacgaac gggacgacca ggccggccac cacctcgaac cagaagaagc ccgcgtaggg
      841 gcccgtgagg agccggcggg cggggtccgc ctcgaagccc gccccgggat acgccgccgt
      901 gagcatctcc gagaacacga ggaacgcctc gacggcgatg aacacggcca gcatcccgcc
      961 gaggtagctc accaggtcgt ccttgaagcc gaccttgccc gtgcgccgca cgatgagcgc
     1021 cagcacgatc atgagcgcgg tgcccgagac aagggccgaa gcgatgaaca gcggcgccat
     1081 gatcgccgag taccagaacc cgcgcgcgat ctgcaggccg aagatccagg cggtgatgga
     1141 gtgcaggagg atcgccgccg ggagcccgac ggccgcgagc acgcctttga gccgctcgtc
     1201 gcggcgcgcg tccgcgaggg acgtgccggt gccgagtgcg agccggctgc cgcgagcagc
     1261 caggtccgag cgggtgtaca cccacacgta ggtcgccgag atgaggaagt acacggtgac
     1321 gatggtgagg tcccagatga gcggcgacat gagctgcggg tagcgcaaca ggtgccaggc
     1381 gcggtccggg cggccgatgt ccgggagcag gagcagggcg gcgatcatga ccgccacggt
     1441 cgcctccagc acggcgatgc gcaccacggg cttgagttgc ttcgcgccga agagccggcc
     1501 ggcggaggcg acgatcagcc cgccggcgga gagtcccacg aagtacatga acatggtgat
     1561 gtacaggccc cacatcatgg ggttgcgcat gttggtggcg gccagcccgt tggcgagctg
     1621 gtagacccag gcgccgagcc ccacggcgat cccgagcgcc aggaggccga tccagagcct
     1681 cagggcccgc cgctcgcggc cctcggccgg cgccgcggcc ggtgtgacga cggtgctcat
     1741 gtgtccagcc ccctcttgcg gcgcggcggc aggtagtaca cgcgcggctc ggtaccgcgc
     1801 tcctccagga gcatctcgcc gctgcgttca cggatgagcc ggctcacgcg gctgtccgga
     1861 tcgttgaggt cgccgaagat gcgcgcctgc gcggggcagg accacacgca cgagggcacc
     1921 tgcccctccg ccaggcggtg gacgcagaag gtgcacttct ccatggtgcc gacgggccgc
     1981 ggcggcacca tcccggtgtc gaagcccgtc ggccggtcgg gctcgcccca gttgaacgtg
     2041 cgcacgccgt acgggcacgc gaccatgcag aagcggcagc cgatgcagcg ctgcggatcc
     2101 tgcatcacga ccccgtcttc gcgcgggtag gtggccgcca cggggcagac cttggtgcag
     2161 gccgggttct cgcagtgctg gcaggcgagc ggcagccagt gcatctccag ctcgccgttg
     2221 acctctctgg gggtgtcgag cgcatcgccg atggtgtcga tgcggttcca ccacatgccc
     2281 agcggtacgt cgttctcaga cttgcagatg acgcagcacg accagcagcc gatgcagcgc
     2341 tcctggtcga cgaccatccc ccagcggggc tcgtcgctcg gctgctccag ctcgccgtac
     2401 gctcgcgacg ccggcggcag cgcctcgacg ggcgagacct tgtcagccat gggtcacctc
     2461 ctgtacggac ggcgccgggg ccgcggcgac gtcgggcgcg gccgccgcgg cgatctccgc
     2521 gtcgataccg tcgccgatcc tcgtgacctc gcagcggcag tccttccagc cggtcgaggg
     2581 gcaccacatg ttggccacgt agtggatctc gtggatgggg ttcacctcgg aggaggtgag
     2641 ctcgttcacg ctcttgccgc cgccgagctg cctgctccac cagccctcga agagggtggc
     2701 gctgccgggc cgggcggcgg gcgtcaggtg cgcccaggcg cggacactcc cgcgggtgtt
     2761 gcgcagctcc acgaggtcgc cctcgccgat gccgcgctcg ccggcggtgc tcgggtgcag
     2821 cagcaccggc gcccggccgc cggtgatctc gttgagccag gccacgttgg cgtagctcga
     2881 gtggatgcgc cacttggagt gcggcgagag cagggcgatc gggtactccg cgggatcgtg
     2941 cacctcgtcg tcgtgcggcg gcttgtacgt ggggaccgct tcgcccagct ccagaaagcg
     3001 gtcctcctcc ttgtagaact cggcacggcg cgtggggatg aacgccgccg tcgcctccag
     3061 cggagccggc agcgactgcg gcgggaacgg cacgagctcc tcgacctggc gcacgaacgc
     3121 gacgtcgggg tcgggcacgt ccagtcgcac cggccccgcc ttcagctgct cgagcgtgat
     3181 gccctcggta ccctcgccct gggcgagcat cgcctcgatc gcctcttccg ccgtcacgtc
     3241 gaagtactcc tcgaacaacg ccgggtcgat gcgcttgacg atctcctgcc acatccacag
     3301 ctccggccgt gactccccca cgggggggat cgcctcctgc tggatctgca ggtaggggtg
     3361 cagcggcgtg gccgtgaggt cggtcttctc gtaccaggtg gccgccggca gcacgatgtc
     3421 cgcccacttc gcggtgtcgg tcatctggtg gtcgaccacc acgacgagtt cgcagccgtc
     3481 cagcgtcttg tgcaggcggt tcacgtccat ggcctgcagg aacatgttgg cgaaggtgca
     3541 gatgagcgcc ttgaacccgt tctcgggata cgggaccgtc tcgtgcatgg tctcggtgcg
     3601 cccccggacg aagtacacgc tgggcacgat cttggctttg gtctcgccca gcgcccacca
     3661 cggcgaggta tccacgcgca ccttgtactg gccgacgtag accgagaagc cgccgcccga
     3721 acggccgacg ttgccggtga gcgaggcgag cagcgcgaag gcgcgcccgg tgaggtcgcc
     3781 gtggaaccag tggttgctgc cgccgcccat gatgacggcc gccggacgct cggccgcgaa
     3841 ggcgtcggcg acgcgcacga tcgtctcgac ggggaccccg gtgatctcgg cggcgcgctc
     3901 cggcgtccag ccgtccagct ccgcgcgcac gagcgcgaac ccgggcgtca cggtgacgcg
     3961 cgacccgtcg gccagcttca cctcgtactc gccgtcgagc gcggcgtcca cgcctgccgg
     4021 gatacccagc cgttccgtgc cgatgaccgc cgcctcgccg cgcacggtgt cccaggcgac
     4081 gtagcggtcg ttcggcagcg gcacgccgtg cgccgggctc tgggccgccg gcgtgccggg
     4141 gaccgtcccc gccggcggcg cctgcctgcc gtagtcgccg aggccgacgc cggcggccgg
     4201 gcccggatca cgggcctcgc cggcggccag ctcgtccgca cgcagccgct tgcccgtgtc
     4261 ggtgcgcacg aggagcggcg cgtcggtgta cgtgcgcatg aagtcccagt cggccaggtc
     4321 gcgggccacg atccgattgc agagggcgag gccgaacgcc gcgtcggtgc cgggcttgat
     4381 ctgcacccag gcgtcggcct tggccgccgt ggacgagaac gtcgggtcga cgaccacgag
     4441 gcgcgcgccc cgctccaccg cgtcgtggat gaagtgcacg tcggggatgc gcgtctcgag
     4501 cgggttcgca ccgatcagca ggatgaagcg gctgttggcc cagtccttgg actcgtgctc
     4561 ggcgttctgc acgccgaacg tgatcggcat gcccattggc aggtcgccgt tgaagtcgaa
     4621 gctcgtgccg tggaccatgc ccagcgcggc acaggcgcga taggccgcgc ccttctggat
     4681 gtagcccgaa cccggcacct ggctgaagac gtggaccgcg tcccagcccc actcgtctcc
     4741 gatgcgctgc agctcgccgg cgatcttctc gaggacctcg tcccaggtgg cctcgcggaa
     4801 ctccccactc ccccgctccc cggtgcgcac gaggggcgtc tggatgcggt cggggccgtg
     4861 gatgagcagg tggtaggaga tgcccttcat gcagccgcgg gggttgtacg cggcatccgg
     4921 gtaatccgcc gcctgctgga ccttgacgac gcggccgtcg cggacgaagg ccagctgccc
     4981 gcacgagccg gtgcagttcg gagagcaggt cgtgcgcacc acgcggtcga cggggagcga
     5041 gggctgcacg tcgtcgcggc cggctgaggc gagtggaccg agcgcgccgg ccgcggcggc
     5101 gctccctgcc gcgacccccg cgccgacgag gaactgccgc cgggtggcct tcatgcgtcc
     5161 gtcctccccg cccgcgcgcc gatgcgcctg gccgcggtct gtacctctcg ccggcgcgcg
     5221 gcggcctcgg cgcaggtcgc gtcgagcgtc gtgtgcgtcg ccgagccgca gagggcgcag
     5281 cgcccggcgc tcgtgccgcg cgcggccggc cgaagggccg gggccgccgc cgccacggcg
     5341 ggctcgaggc gctcggccgc ccggacggcg agcgcgccga gcgcggacac tccggcagcc
     5401 accacgaatc tgcgtctggt catggccatc gggttcctcc atctctggga atcggtgccc
     5461 ggagccgcat gcgcggcgct cctctttccc gggagaataa caaacactaa gtaacttagc
     5521 aatattattt cggcgggaac tgaccttcgc ttctcgggct cttccgcggc tgcaacggca
     5581 acgctcgcgg cgtacgcact tgcttgccgc gggggcaggc tctacagtga tgtgtgcgac
     5641 agcgtcaacg gctgcgcgta ccgcagccgc ggcgcgtgga gagacgggag actaggacat
     5701 gccaaacctc ggtgcaccag aactcatcat catcgctctg gtcatcctgc tgctcttcgg
     5761 cgcgacgcgg ctgccgaagc tcggcaagtc gatgggccag agcatcaagg gcttcaagca
     5821 gggcttgaac gacgacgccg acgacgacga gatcgtggac gtgaagcgcg acgcgacggc
     5881 cgaggacaag gacgtcaagg cctcctgacc ggccccgaga gaacgctccg acggcccgcc
     5941 gctggcgggc cgtcgtcgtt aaggaaccgt cgtcgtcggc gtcgccgggg cgggcagccc
     6001 gccgtcagcg gctgagcagg cagacggcgt gggcggcgat gccctccccg cgcccctcga
     6061 accccatgcc ctcggtggtc gtcgccttga cgctcaccgc gtcgctggcc acgcccagag
     6121 ccgcggccag gcgctcgcgc atctcgaggc ggtagccgga gagccgcggg cgctcacaga
     6181 cgatcgtcac gtcggcgttc tcgacccgcc acccgttcgt ggtgagccac gcgatgaccc
     6241 gctcgagcag cgccatgctg gaggcgccct cgaacgcggg gtccgtgtcc gggaacagct
     6301 cgccgatgtc gccgaggccg gcggcgccga gcagcgcgtc catcagcgcg tgcacggcga
     6361 cgtcggcgtc ggagtgcccg gcgagcccct gcgcgtgatc gatggccact cccccgagca
     6421 cgaggtcgcg gccgtcggtg aagcgatgcg cgtcgtagcc gccgccgacc ctaaacgtcg
     6481 gtctcgacct cccacgagag gagcgccccg gcgagcggga tgtcctgcgg cgtcgtgatc
     6541 ttgacgttcg cgggcgtgga gggcacgagg cgcacggagt agccgccgcg ctcgaggagc
     6601 atggcgtcgt cggtcgcgcc gttcaggtca gtccgcgcgt acacgtcgcg gagggcggcg
     6661 gagcggaaga cctgcggcgt ctgcgccgcc cacagccctg cgcggtcgac ggtgccggcc
     6721 accgcgccgc cctcgccctg gcgcttgagc gtgtcgctga cctcgacggc ggggacggct
     6781 ccgtcggtct cgcggagcgc cgtcacgcag gcctcgaaca cgtgcttgga gaacagcggc
     6841 cgcgcgccgt cgtggacggc gatgacggtg gcgctgtcag gcaccatgac gagcccggcg
     6901 cgcacggaat ccgaacgcgc cctgccgcct tcggcgatca gcaccggctt ggaatcgtcg
     6961 aacagcatga gctcgttggc gaagcgcacc gtgtgcgacg ggtgcagcac caccacgatg
     7021 ccgtccacgc agctcatcgc cagcaggcga tggaccgagt gggagaggat cgtctccccg
     7081 ccgagcagca ggaactgttt cgattcaccg tcgggcagcc ccatccgctg gctttcgccg
     7141 gcagccgcga cgatgaccca ggtctctccg gcctgctggt cctccacgtc catcccctct
     7201 cactcagttc tctgccggcg gcggagcgct cacgcggctg aagatcatct tgcccgaggg
     7261 gctctgcagg acgctcgtca cctcggcttt gacgcgcgag ccgaccaggc tgctgccggc
     7321 ctcgacgacg atcatggtgc cgtcgtcgag gtagccgacg ccctggtccc gctcgcgccc
     7381 ttctctcagg atcttgacct cgatgatctc gcccggcagc accgccggct tgaccgagtt
     7441 ggcgaggtcg ttgacgttga gcatcttgac gccgtggatc tcggcgacct tcttgagcgt
     7501 gtagtcggtg ctgacgatca tcgccccggt gtcgtgcccg agccggacca gcttggcgtc
     7561 caccgcctcg atgtccggat agtcggcgcc gtcga
//
LOCUS       DASUMX010000062        14420 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_592601, whole genome shotgun sequence.
ACCESSION   DASUMX010000062 DASUMX010000000
VERSION     DASUMX010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 14420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14420
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592601"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /locus_tag="VFZ86_02455"
     CDS             complement(<1..535)
                     /locus_tag="VFZ86_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641181.1"
                     /translation="MSDNLMSRRKFLAGTGLVLGAASVSGVALLKDADPAEAAGTAIP
                     WAYPTVPADQPNPEAVARRAFEIYFKSGCAEAVWYSCVEALAAIPGANQADWASLPMN
                     MFRFGGGGIAGWGTICGTLNGAAAFTSMAVGQNLAADGVTKEWTHRNNLINGIFQYYA
                     ETPLPTNNTYKSTQGALG"
     gene            complement(743..1843)
                     /locus_tag="VFZ86_02460"
     CDS             complement(743..1843)
                     /locus_tag="VFZ86_02460"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family peptidase"
                     /protein_id="HEX5641182.1"
                     /translation="MDHALVIEASGSHREVGRQIGEAARDSIAWGLDAYAARFEGLAG
                     FGFGEAVDRTRAYLRPAEDYTPQALEQLRGMAEAATVPFEHLFALNCSEEFTCRADAS
                     WPVEHCTSFAVVAGGQPVAGHNEDWYPEEIEAQTIRIVRVKGRWSAAAYISAGPAYNL
                     PITGVTAAGFSSAANTVYYRDERAGVPNNLLLATVLCQPDLEHARDVIAAAPRARGSN
                     HLLCDARGRVWDLETSAERWAFLDGSPPPPDAASSGATALFTHTNHYVSPELAPGDAS
                     RSEGSRLRRERAERLLLDGLAAGTDLVELGRRVLCDHENAPSSICDHWEEDDPDPDQS
                     VTTASMVWEPAEGRAHIAAGQPCGSEYVTFDL"
     gene            1903..3762
                     /locus_tag="VFZ86_02465"
     CDS             1903..3762
                     /locus_tag="VFZ86_02465"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HEX5641183.1"
                     /translation="MGGDRDFRFSPRPNRAAEIRWRPWGEPAFAEARRLGRPILLSLS
                     AVWCHWCHVMDETSYSDPRVIAAVNEHFVPVRVDNDRHPDVNRRYNMGGWPTTAFLAA
                     SGDVLTGGTYLPPEQMLESLARVKAFFDANQAKILALGGERAADGDGTAMARLTGMPL
                     RGEQAMDALEGDPEVPGDISAEVALQVVRGFDPVCGGIGAEPKFPQPDVYSFALAYGL
                     LRGAGDPDHVPEGGGALLRPTRVLEVVRATLTAMAGGGLYDGVEGGFFRYATQRDWSV
                     PHYEKMLEDNARLILLYLEASLHAEEHDLGDAAAFRRVAEGSIDYLLATLWRDDVPAF
                     AGSQDADEQYYLLDAGGRAGLPAPFVDPTVYVDWNALAARALLRAAPVLGRPELVDRA
                     LRLLEYLVTTARRGDAMAHFVHPDGAVGDGAPLLGDQATVAAAELDAYEVTGDRRWLR
                     RARGLARWTGEHLCAPDGRLLDRLAVPGESAGLLAQPVPALDENAAMAEVLLRLEADT
                     GEARLRERALEILAAWATQYEQYGVAASAYAQALLRYLERPDHIAVVGRRDDEEARRL
                     HAAALVAPRPLRTVQWLDPGDAQDAERMRDTGLPPAPAAAYVCRGRSCSLFRP"
     gene            3858..4493
                     /locus_tag="VFZ86_02470"
     CDS             3858..4493
                     /locus_tag="VFZ86_02470"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEX5641184.1"
                     /translation="MITLYQTEWCPYCHRVRQVLTELGLTYTAVNVPYANEDRVELLA
                     IAGQDGVPVLTDGDKVYGDSDEIVEYLRATYPEPEDADEHAAHGAWRAARMVSLAPRA
                     ALARLKELLEEKGFVVLAQVKGPKINERLPQEYAILQVTVPVAAVKALDVDPLAPVAI
                     MFPIAVIPTEDGKSVVAAADPVGQVWLYGEPDLNRVQGAVKKRLAELFSEL"
     gene            complement(4549..5361)
                     /locus_tag="VFZ86_02475"
     CDS             complement(4549..5361)
                     /locus_tag="VFZ86_02475"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5641185.1"
                     /translation="MSRDATVPAATAGRRTTHPGGLRVLLKLTWIELKLFLREPMTIV
                     FTFAFPVVLMFVLGEVFGNQPGGANDRVFNGMGAMNYYAPAYIAVVTMSVGVIMLPAR
                     LTDYRSGGVLRRFRASLVPIWAVLGSQVLAALVLGVIGAVLVAAFAAVFYPVHFPDSL
                     GLLAAGFVLCSAAFAAIGTLLGAVMPSPRAAQGAGLMLFFVMLFLSGASVPMEVMSDT
                     MRRIGDFLPLTYGVDLLQGAWSGGSWDWTAAIVLAGITVVAGFLSLRVYRWG"
     gene            complement(5438..6349)
                     /locus_tag="VFZ86_02480"
     CDS             complement(5438..6349)
                     /locus_tag="VFZ86_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013736117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5641186.1"
                     /translation="MPPVITVEHLRKVYGHHVVAVDDVSLEVEQGEIFGLLGSNGAGK
                     TTTVECVQGLRTPTAGTVRVLGKDPRTQAGDLRGRMGCQLQESRLPDHMRVWEALDLF
                     ASLAPGAVDWRMLVEQWGIAEKRNASYASLSGGQRQRLFIALALVSAPEVVFLDEMTT
                     GLDPAARRVAWDLIEQVRDRGTTVVLVTHFMDEAERLCDRVAVVDKGRIIAADTPRGL
                     VVRYDPLEQVVFSCDLPEVEWLRGVPGARSVTRRGPRIVVEGTGPLLAHVAAALVDRG
                     LTPDDLRVIEPSLEDVFLTLTGHLVED"
     gene            complement(6448..7398)
                     /locus_tag="VFZ86_02485"
     CDS             complement(6448..7398)
                     /locus_tag="VFZ86_02485"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF004344.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEX5641187.1"
                     /translation="MSEEIERLQSRLKKLQHESRRHDTWVTVEIARHKERPRTRDYID
                     FLIDGFEELKGDRLRADDTAIVGGLGWFRGRAVLVVGHQKGRTLDELTQNNFGMANPE
                     GYRKAQRLFKLAERHGLPVLTFVDTPGAYPGVAAEEGGQARAIAETMLCMARLTVPVI
                     ATIIGEGGSGGALALAVADKVLMLENATYSVITPEGCAAILWRDADFAPQAAEALRPT
                     ASQLLELMAVEQVVEEPRGGAHHNPELAAQRLGEAIAEALAELDQTPALERRRQRRER
                     FRRLGQWSDPEQPPGNLSLFGGYSVPVERAGAARPEVTEP"
     gene            complement(7331..8332)
                     /gene="accD"
                     /locus_tag="VFZ86_02490"
     CDS             complement(7331..8332)
                     /gene="accD"
                     /locus_tag="VFZ86_02490"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013615376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HEX5641188.1"
                     /translation="MNVTVQIERRNNEPARPEWICPSCHQGARHSEVRANLMVCPSCD
                     HHLRIGARERIQQLADEGTFHELWSMVTTLDPLGFVDLETYPERVREAQAKTGLQEAI
                     VTGTASIDATPCVLAVMDFGFMGGSMGSVVGEKLWRSAELASGEDLPLVAVCTSGGAR
                     MQEGILSLMQMAKTSCAVDLINEATSPFVTVLADPCTGGVVASFATLADICVAEPGAR
                     LYFTGPRVIQQTTQEALPQGFSTAERNLELGHLDAVVPRRDLRVKVGNYLRLLGGGEE
                     PDRGEGPEDGTGRSGRLAQAAAERARTLATYARRSLVRGDRETPEPPEEAAAREPSP"
     gene            complement(8378..9622)
                     /gene="fabF"
                     /locus_tag="VFZ86_02495"
     CDS             complement(8378..9622)
                     /gene="fabF"
                     /locus_tag="VFZ86_02495"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009631537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEX5641189.1"
                     /translation="MADQRRVVVTGLGVISPIGNEVDTFWRRLVAGDSGVGEITRFDT
                     TGYKVHIAAEVQGFAAADYIDKRRARRLDLFSQYAVAAAIDAAADAAFDPRPEAERVG
                     AVMASGVGGLQTLHVETDKLLNQGPDRTNPLLVPMMIPNMGAAHVSLELGTKGPLSTT
                     CTACAAASDAIGYAGRLIRHGDAEVMFAGGSEAPISPVGVSGFAAARALSTRNDDPQG
                     ASRPFDANRDGFVIGEGAGVLVLEELEHALARGARIYAELAGSGMSSDAFHMTLPDES
                     GESQARAMLMALQEAGLDPSSVDYVNAHGTSTGPGDIAETRAIKQALGEHAYEVAISS
                     NKSMIGHCLGAAGALEAVATVLTIVNSLIPPTINLTDPDPECDLDYVPLESRFGKVDV
                     AASNSFGFGGHNVALVFRRYDG"
     gene            complement(9640..10404)
                     /gene="fabG"
                     /locus_tag="VFZ86_02500"
     CDS             complement(9640..10404)
                     /gene="fabG"
                     /locus_tag="VFZ86_02500"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007488090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEX5641190.1"
                     /translation="MTPLTAGLDLTGATALVTGAGRGIGRGIALALAGAGADVAVNDF
                     ADEAACAAVVAEVEALGGKGLVVMGDVSDEGQVNEMVERAETELGPLSVVVNNAGITR
                     DNVTMMMDPADFDAVIATHLRGTFLVSKAAARRMFRRRTGCIINLSSVVGRRGNAGQA
                     NYAAAKAGIIGLTKSLAKELGARGVRVNAIAPGYIDTPMTQALPEETRQMIVEATPLK
                     SIGTTEDVAMAVVYLASPAARFITGVVLPVDGGLGI"
     gene            complement(10401..11333)
                     /gene="fabD"
                     /locus_tag="VFZ86_02505"
     CDS             complement(10401..11333)
                     /gene="fabD"
                     /locus_tag="VFZ86_02505"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEX5641191.1"
                     /translation="MKNLVVMFPGQGSQAVGMGCDLAAADPAMRDAYAEASEVLGYDL
                     QDLCCQGPIDRLSRTDFTQPALLAASVGMFRILRREGLEFDVAIGHSLGEFSALVATG
                     AVDFADALRIVRRRGQEMLRAGHASPGGMAAVIGLDDAVVEALCAEQDGLWPANFNCP
                     GQVVISGTKPALEVLSEKAKAAGAKRVISLAVSGAFHSPLVQSALEPLRDEFERTEWR
                     TPEPAFFSACSVAFETGDFNELLQVQLVSPVRFSQSVSTLYAAGYDAFLEVGPGAVLT
                     GLVKRIEPQAQAARVSDMETLLALRADGRYLEDA"
     gene            complement(11343..12350)
                     /locus_tag="VFZ86_02510"
     CDS             complement(11343..12350)
                     /locus_tag="VFZ86_02510"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEX5641192.1"
                     /translation="MIGLRRGLVRGARISALGVHVPERVLTNDEISQFLDTSDEWITT
                     RTGIRERRIARVDEAASDLGEVAARRCLESGGLDAAELDLVIVTTISPDHIMPATASI
                     VADKIGAGKAGAFDVQAGCTGFVYGLAIATAFVSANIYTNVLVVGAEVLSKMVDWEDR
                     STCVLFGDGAGAVLVQPTDNGSIFSFDLGNDGSGAPNLLVPAGGSRMPATYDTVRDHE
                     HAMHMTGSEVFKFATRTVVDSCQKVLSDAEITVDDVDLFVPHQANIRIIESASRRLGM
                     REGQVFANLDRYGNTSCASIPICLHEASESGRLKKGDTLLLAGFGAGLTWGSCLTKWE
                     L"
     gene            complement(12347..12856)
                     /gene="fabZ"
                     /locus_tag="VFZ86_02515"
     CDS             complement(12347..12856)
                     /gene="fabZ"
                     /locus_tag="VFZ86_02515"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEX5641193.1"
                     /translation="MRLTNEEVLTLLPHRPPFLLVDEVVDGEPGARCVAVRTLRDDDW
                     WFAGHFPGNPVMPGVLIVEALAQTATIAAASAGSEAAAGGDTLPSADAAPGGDKVGLF
                     AGIDKVRFKRVCKPGDTLTLEAEIVAVHGPVGRAKVKATVDGQLACRGELMFAIVDAA
                     SVAAGEAKL"
     gene            complement(12853..13278)
                     /locus_tag="VFZ86_02520"
     CDS             complement(12853..13278)
                     /locus_tag="VFZ86_02520"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX5641194.1"
                     /translation="MDCSYFRSRLNQFLDGELGYLDVAEFQSHLSFCPHCAAELSQLS
                     EVRGAMAVWGEVELAPPPGFAERVAAAAALEARPGEATLGRVVGDVLDTLDATLGRLP
                     LPGGRTVPVKNVLGYGIAATAVLIQVERYLRRTRELKPL"
     gene            complement(13278..13844)
                     /locus_tag="VFZ86_02525"
     CDS             complement(13278..13844)
                     /locus_tag="VFZ86_02525"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX5641195.1"
                     /translation="MSTSNLHKWAMLPDPTPADLARAAAGDVDAFKTIVERYQGMVYS
                     VAYNVLGSHTDAEDAAQEAFLRCYRKLAQYRGEATFSTWLFRLALTASVDYQRRERRR
                     PEPVEVPDVADGGQAELGDGISAATLTAALRDLPDDYRVPTVLRDIYGLPYQEIADLT
                     ARPLGTVKVMVHRGRASLRLRLRAEREA"
     gene            complement(14004..>14420)
                     /locus_tag="VFZ86_02530"
     CDS             complement(14004..>14420)
                     /locus_tag="VFZ86_02530"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HEX5641196.1"
                     /translation="RATLLHDVGYLESGLQSSYESIVLGDELVGYARQFMREVPVDDY
                     SLALDEIKAVGPGGSHLGTKYTRRHHREFWTPALLDHAVHDRWAAEGATTLGARVRER
                     LAGLRAAGRAFSLTAAQDARLDALLEAALADRAAGV"
BASE COUNT         2072 a         5043 c         5208 g         2097 t
ORIGIN      
        1 ggccgagcgc gccctgcgtc gacttgtacg tgttgttggt cgggaggggt gtctcggcgt
       61 agtactggaa gatgccattg atcaggttgt tgcgatgcgt ccactccttg gtgacgccgt
      121 ccgcggccag gttctggccc acggccatgc tggtgaacgc cgccgcgcca ttcagcgttc
      181 cgcagatggt cccccagccg gcgatgccgc cgccgccgaa gcggaacatg ttcatcggca
      241 acgacgccca atcggcctgg ttcgcaccgg gaatggcggc gagggcctcg acgcaggagt
      301 accagacggc ctccgcgcag cccgacttga agtagatctc gaaggcgcgc cgggcgacgg
      361 cctctgggtt cggctggtcc gccggaactg tcggatacgc ccacgggatg gccgtgccgg
      421 cggcctccgc cgggtctgcg tccttgagca aggccacgcc ggagacggag gcggccccga
      481 gcacgaggcc ggtcccggcg aggaacttgc gccgagacat caggttgtcg gacatacgct
      541 cgactccttt cactgctttg ccccgcgctc cgcgaagcgt tcggacccat gtcgcaagtg
      601 aaggcgccct tgaaggttcc ttcacatatg cgttgaagcg catggaagct accacattca
      661 ccttagaatg tgaagcggtt cacaaaggca acgacgtcgg tcgacttcgt cgggaatgcg
      721 gcgctcgaag cccgggcgga ccttagaggt cgaacgtcac gtactccgac ccacagggct
      781 ggccggcggc gatgtgtgca cggccctcgg ccggctccca caccatgctc gccgtggtca
      841 cgctctgatc ggggtcaggg tcgtcttcct cccagtggtc gcagatgctg gacggcgcgt
      901 tctcgtggtc gcagagcacg cgcctgccca gctcgaccag atcggttcct gccgcgaggc
      961 cgtcgagcag caggcgctcg gcccgctcgc gccgcaggcg cgagccctcc gagcgcgaag
     1021 cgtcgcccgg cgcgagctcc ggcgacacgt agtggttggt gtgcgtgaag agggcggtcg
     1081 cgccggagct cgccgcatcg gggggcggcg gcgagccgtc caggaacgcc cagcgctcgg
     1141 cgctcgtctc cagatcccac acgcggccgc gggcatcgca gagcaggtgg ttggacccgc
     1201 gcgcccgcgg cgccgcggcg atcacgtcac gcgcgtgctc gaggtcgggc tggcagagca
     1261 ccgtggcgag cagcaggttg ttgggcacgc cggcgcgctc gtcgcgatag tagaccgtgt
     1321 tggcggcgga gctgaagccg gccgccgtga cgccggtgat cggcaggttg taggcaggac
     1381 cggcgctgat gtaggcggcg gccgaccagc gccccttgac gcggacgatc cggatcgtct
     1441 gtgcttcgat ctcctccggg taccagtcct cgttgtgccc ggccaccggc tgcccgccgg
     1501 cgacgacggc gaacgaggtg cagtgttcca ccggccagct cgcgtccgcc cggcaggtga
     1561 actcctcgct gcagttgagc gcgaacagat gctcgaaggg gaccgtggcc gcctcggcca
     1621 tcccgcggag ctgctcgaga gcctggggcg tgtagtcctc ggccggccgc aggtacgcgc
     1681 gggtgcgatc gacggcctcg ccgaacccga agccggcgag gccctcgaac cgtgcggcgt
     1741 aagcgtcgag accccaggcg atgctgtcgc gcgcggcctc gccgatctgc cgccccacct
     1801 cccggtggga tccggaagcc tcaatgacga gcgcgtggtc cacggaccta tcctacgtcc
     1861 agtcattgat tcggaccacg ggcctttggg aatgccgtgg ccatgggcgg cgaccgcgac
     1921 ttccggttct ccccgcgtcc gaaccgggcg gcggagatca ggtggcgccc gtggggcgag
     1981 cccgccttcg cggaggctcg ccggctcggg cgccccatcc tcctgtcgct ctccgccgtg
     2041 tggtgccact ggtgccacgt catggacgag acgagctact ccgacccgcg cgtgatcgcc
     2101 gccgtcaacg agcacttcgt gccggtccgc gtcgacaacg accgccaccc cgacgtgaac
     2161 cgccgctaca acatgggcgg ctggccgacc acggcgttcc tcgccgccag cggcgacgtg
     2221 ctcaccggcg gcacctatct cccaccggaa cagatgctcg agagcctggc gcgcgtcaag
     2281 gcgttcttcg acgccaatca ggccaagatc ctcgcgctcg gaggcgagcg ggccgccgac
     2341 ggcgacggaa cggccatggc ccggctgacc gggatgcctc tccgcggcga acaggcgatg
     2401 gatgcgctcg agggagatcc cgaggtcccc ggcgacatct cggccgaggt ggcgctgcag
     2461 gtcgtccgcg ggttcgaccc ggtctgcggc ggcatcgggg cggagcccaa gttcccgcag
     2521 ccggacgtgt actccttcgc gctcgcctac ggactgctgc gcggagccgg cgacccggac
     2581 cacgtgcccg agggaggcgg cgcgctcctg cggccgacgc gcgtgctcga ggtggtgcgc
     2641 gcgacgctca ccgccatggc cggcggaggg ctgtacgacg gcgtcgaggg cggcttcttc
     2701 cgctatgcca cccaacgtga ctggagcgtg ccgcactacg agaagatgct ggaggacaac
     2761 gcccggctga tcctcctgta cctcgaggcc tcactgcacg cggaggaaca cgacctcggc
     2821 gacgctgcgg cgttccggcg cgtcgccgag gggtcgatcg actatctgct ggcgacgctc
     2881 tggcgcgacg acgtacccgc cttcgccggc agccaggacg ccgacgagca gtactacctg
     2941 ctggatgccg gcggccgggc agggctgccg gcgcccttcg tcgaccctac ggtctacgtc
     3001 gactggaacg cgctggcggc gcgggctctg ctccgggcgg cgccggtgct cggccggccg
     3061 gagctcgtcg accgcgcgct gcgcctcctg gagtacctcg tcacgacggc gcggcgcggc
     3121 gacgcgatgg cgcacttcgt gcatccggac ggggccgtcg gcgacggcgc gccgcttctc
     3181 ggcgaccagg cgaccgtcgc ggcggcggag ctcgacgcct acgaggtgac gggcgaccgg
     3241 cgctggctgc gacgcgctcg cggcctcgcg cgctggaccg gcgagcacct gtgcgcgccg
     3301 gacgggcgcc tgctcgacag actcgccgtg ccgggagaga gcgccggcct gctggcgcag
     3361 ccggtgccgg cgctcgacga gaacgcggcg atggccgagg tcctgctgcg cctcgaggcc
     3421 gacaccgggg aagcccgcct ccgcgaacgc gcgctcgaga tcctcgccgc ctgggccacg
     3481 cagtacgaac agtacggcgt cgccgcctcg gcgtacgcgc aggcgctgct ccgctatctg
     3541 gagcggccgg accacatcgc ggtggtcgga cgccgcgacg atgaggaggc gcgacggctg
     3601 cacgccgccg cgctggtcgc accgcgaccg ctccggaccg tccagtggct cgatcccggg
     3661 gacgcgcagg acgcggagag gatgcgcgac accgggctgc cgcccgcacc cgcggcggcc
     3721 tacgtgtgcc gcggccgctc ctgttcgctc ttccggcctt gagcgagaag gtgagccccg
     3781 ctgtgggata agctggtgac ttcgccagcg cacgaccggc ggcaccgttc tctgttctca
     3841 cgcttcacgg agggacgatg atcacgctgt accagactga gtggtgcccg tactgccatc
     3901 gggtgcgtca ggtgctcacg gagctcgggc tcacgtatac cgcggtcaat gtcccgtatg
     3961 cgaacgagga ccgtgtggaa ctcctggcca tcgccggcca ggatggtgtc ccggtgctca
     4021 cggacggcga caaggtctac ggagactccg acgagatcgt cgagtatctc cgtgcgacct
     4081 acccggaacc ggaggacgcc gatgagcacg ccgcgcacgg cgcctggcgc gcggcgagga
     4141 tggtgtcgct cgcgccgcgc gccgccctcg cccggctcaa ggaactgctg gaggagaagg
     4201 gtttcgtcgt cctcgcccag gtcaaagggc ccaagatcaa cgagcgcctg ccgcaggagt
     4261 acgcgatcct gcaggtgacg gtgcccgtgg ccgcggtcaa ggcgctcgac gtcgacccac
     4321 tggcgccggt ggcgatcatg ttcccgatcg ccgtcatccc cactgaggac ggtaagagcg
     4381 tcgtggccgc ggccgacccc gtcgggcagg tgtggctcta tggcgagccc gacctgaacc
     4441 gggtgcaggg cgcggtcaag aagcggctcg cggagctgtt ctccgagctc tgacggcagc
     4501 cacacggccc acgccgggcc ggagcgggag aggcggtccc gccggacctc acccccagcg
     4561 gtagacgcgc aggctcagga accctgcgac gacagtgatg cctgcgagga cgatcgcggc
     4621 ggtccagtcc caggagccgc cgctccacgc cccctgcagc aggtcgaccc cataggtgag
     4681 cggcaggaag tcgccgatcc gcctcatggt gtcgctcatg acctccatcg gcaccgaggc
     4741 gccgctcagg aacagcatga cgaagaagag catcagcccg gcgccctggg cggcgcgtgg
     4801 gctgggcatc accgcgccga gcagcgtgcc gatggcggcg aacgccgccg agcagagcac
     4861 gaacccggcg gccagcaagc ccagcgaatc ggggaagtgc accgggtaga agaccgccgc
     4921 gaaggcggcg accagcacgg cgccgatcac gccgagcacc agcgccgcca gcacctgcga
     4981 gccgaggacg gcccagatgg ggacgagcga agcccggaag cggcgcagca cgcccccgct
     5041 gcggtagtcg gtgagcctgg cgggcagcat gatcacgccg acggacatcg tgaccacggc
     5101 gatgtaggcc ggagcgtagt agttcatcgc acccatcccg ttgaacacgc ggtcgttggc
     5161 gccgccgggc tggttcccga acacctcgcc cagcacgaac atgagcacca cggggaaggc
     5221 gaacgtgaac acgatggtca tgggctcgcg cagaaagagc ttgagctcga tccatgtgag
     5281 cttgagcagc acgcgaagac cgccgggatg agtggtgcgc cgtccggcgg tcgcggccgg
     5341 aaccgtggcg tcccggctca tcagtccgtc cgccgcgggg cccttcgggc cgtcggccgc
     5401 tcactagcac tcgcgcgact gcgtcctgct tccggcatca gtcctccacc aggtgccccg
     5461 tgagcgtgag gaacacgtcc tcgaggctcg gctcgatcac gcgcagatcg tcgggggtca
     5521 ggccgcggtc gaccagcgcg gccgccacgt gcgcgagcag cggtccggtg ccctccacca
     5581 cgatgcgggg cccgcgccgg gtgacgctgc gcgcgcccgg cacacctctg agccactcca
     5641 cctcgggcag gtcgcagctg aacaccacct gctcgagagg atcgtagcgg acgacgaggc
     5701 cgcgcggcgt gtccgccgcg atgatcctgc ccttgtccac cactgcgacg cggtcgcaga
     5761 gccgctccgc ctcgtccatg aagtgcgtga cgaggacgac ggtcgtgccg cggtcacgga
     5821 cctgctcgat gaggtcccag gcgacgcgtc gcgccgccgg atcgaggccg gtggtcatct
     5881 catcgagaaa gacgacctcg ggagcgctca ccagcgcgag ggcgatgaac agccgctggc
     5941 gctggccccc actgaggctc gcgtacgatg cgttccgctt ctccgcgatc ccccactgct
     6001 ccaccagcat ccgccagtcc accgcgccgg gagcgaggct ggcgaagagg tcgagcgcct
     6061 cccacaccct catgtggtcg gggagccgtg actcctggag ctggcagccc atgcgcccgc
     6121 gcaggtcgcc ggcctgggtg cgcggatcct tgccgagcac gcgcaccgtc ccggccgtcg
     6181 gcgtgcgcag cccctgcacg cactcgacgg tggtggtctt gccggctccg ttggacccca
     6241 gcaggccgaa gatctctccc tgctccacct cgagcgacac gtcgtcgacg gccacgacgt
     6301 ggtggccgta gaccttgcgc aggtgttcga cggtgatgac cggcggcatg cgttgaacct
     6361 acccggattg ggccgcggcg gacggcgcag ggcgcgtcgt gaccgcgacg ggagaagaac
     6421 gtcggctgtg agacgtccgg accctgctca gggttcggtg acttccggtc tcgccgctcc
     6481 cgcgcgctcg acggggaccg agtagccgcc gaagagagag aggttgcccg gcggctgctc
     6541 ggggtcgctc cactggccga ggcggcggaa ccgctcgcgg cgctgccgcc gccgctccag
     6601 cgcgggggtc tggtcgagct cggcgagggc ctcggcgatc gcttctccga gccgctgcgc
     6661 cgccagctcc gggttgtggt gggcgccgcc gcggggctcc tccacgacct gctcgacggc
     6721 catcagctcg agcagctgcg acgccgtcgg gcgcagcgcc tcggcggcct gtggcgcgaa
     6781 gtcggcgtcg cgccagagga tcgcggcgca gccctcgggc gtgatcacgg agtaggtggc
     6841 gttctcgagc atgagcacct tgtcggcgac ggcgagggcc agggcgccgc cggaaccgcc
     6901 ctcgccgatg atcgtggcga tgacggggac ggtgaggcgc gccatgcaca gcatcgtctc
     6961 ggcgatggcg cgcgcctgcc ctccctcctc cgcggccacg cccgggtacg cgcccggtgt
     7021 gtcgacgaag gtgagcaccg gcagcccgtg ccgctcggcg agcttgaaca gccgctgcgc
     7081 cttgcggtag ccctcggggt tggccatgcc gaagttgttc tgggtgagct cgtcgagggt
     7141 gcggcccttc tggtggccga ccacgagcac ggcacggccg cggaaccagc cgagcccgcc
     7201 gacgatcgcc gtgtcgtcgg cgcgcaggcg gtcgcccttc agctcctcga acccgtcgat
     7261 caggaagtcg atgtagtcgc gcgtgcgcgg ccgctccttg tgccgcgcga tctcgacggt
     7321 gacccacgtg tcatggcgac ggctctcgtg ctgcagcttc ttcaggcggc tctggagtct
     7381 ctcgatctcc tcggacaagt gaacgccggg cataagtggc aagcgtccga gctcgctcag
     7441 ccgctgcctg agccagccgg cccgaacgtc ctgttccgtc ttcgggtcct tctcctcggt
     7501 caggttcctc accccctccc agcagtcgca ggtagttgcc gaccttgacg cgcaggtccc
     7561 tgcgcgggac caccgcgtcg aggtgaccga gttcgaggtt gcgctccgcc gtgctgaagc
     7621 cctggggaag cgcctcctgc gtggtctgct ggatgacgcg cggaccggtg aagtacagcc
     7681 gcgcgcccgg ctccgcgacg cagatgtcgg caagcgtcgc gaagctggcg accacaccgc
     7741 ccgtacaggg gtcggcgagg acggtgacga acggggaggt cgcctcgttg atgagatcca
     7801 cggcgcagct ggtcttggcc atctgcatga ggctgaggat gccctcctgc atgcgcgcgc
     7861 cgccggaggt gcacaccgcg accagcggca ggtcctcgcc gctcgccagc tcggcgcttc
     7921 gccagagctt ctccccgacg acgctcccca tgctgccgcc catgaacccg aagtccatga
     7981 cggcgagcac gcacggggtg gcgtcgatgc tcgcggtccc cgtgacgatg gcctcctgca
     8041 ggccggtctt tgcctgcgcc tcgcggacac gctcgggata ggtctcgagg tcgacgaacc
     8101 cgagggggtc cagcgtcgtg accatggacc agagctcgtg gaacgtgcct tcgtccgcaa
     8161 gctgctggat ccgctcgcgc gcgccgatgc gcaggtggtg gtcgcagctc gggcagacca
     8221 tgaggttggc gcgcacctcg ctgtgccgcg cgccctggtg gcaggacggg cagatccact
     8281 ccgggcgcgc cggctcgttg ttgcggcgtt cgatctggac ggtgacgttc atgagatgct
     8341 gcgccctctg gccggtgaga ccgggtcgcg gcgccggtca gccgtcgtag cggcggaaga
     8401 ccagggccac attgtggccg ccgaagccga agctgttgct ggcggcgacg tccaccttgc
     8461 cgaagcggga ctcgagcggg acgtagtcga ggtcgcactc cgggtcgggg tccgtgaggt
     8521 tgatcgtcgg cgggatcaga ctgttgacga tggtcaggac ggtcgcgacc gcctcgaggg
     8581 cgcccgctgc gccgaggcaa tggccgatca tcgacttgtt ggacgagatc gcgacctcgt
     8641 acgcgtgctc gccgagcgcc tgcttgatcg cgcgggtctc ggcgatgtcg ccggggccgg
     8701 tcgaggtgcc gtgcgcgttg acgtagtcca cggaggacgg gtcgaggccg gcctcctgca
     8761 gcgccatgag catggcgcgg gcctgcgact cgccggactc gtccggcagc gtcatgtgga
     8821 aggcgtcgct gctcatgccg ctgccggcca gctcagcgta gatacgcgcg ccgcgggcca
     8881 gggcgtgctc gagctcctcg aggacgagca cgccggcgcc ttcgccgatc acgaagccgt
     8941 cgcggttggc gtcgaatggc cgcgaagcgc cctgcgggtc gtcgttcctg gtgctcaggg
     9001 cccgcgcggc cgcgaacccg gacaccccga ccgggctgat cggcgcctcg ctgccgccgg
     9061 cgaacatgac ctcggcgtcg ccgtggcgga tgaggcgccc cgcgtagccg atcgcgtcgg
     9121 aggccgcggc gcaggcggtg cacgtcgtgc tgagcgggcc cttcgtgccc agctccagcg
     9181 agacgtgcgc cgcgcccatg ttggggatca tcatcgggac cagcagcggg ttggtgcgat
     9241 cgggtccctg gttgagcagc ttgtcggtct cgacgtgcag cgtctgcagg ccgccgacgc
     9301 cgctcgccat gacggcgccg acccgctccg cctcggggcg ggggtcgaac gccgcgtcgg
     9361 cggcggcgtc gatcgccgcg gccaccgcgt actggctgaa gaggtcgagc ctgcgcgccc
     9421 tgcgtttgtc gatgtagtcc gcggccgcga agccctggac ctcggcggcg atgtggacct
     9481 tgtagcccgt ggtgtcgaag cgcgtgatct cgcccacgcc ggagtcgccg gcgacgagac
     9541 gacgccagaa ggtgtcgacc tcgttgccga tgggcgagat gacgcccagg ccggtgacga
     9601 cgactctgcg ctggtctgcc atggtccgcg ccggccccgt cagatcccga ggccgccgtc
     9661 gacgggcagg acgacaccgg tgatgaagcg agccgccggc gaggccaggt acacgacggc
     9721 catggccacg tcctcggtgg taccgatgct cttgagcggt gtcgcctcga cgatcatctg
     9781 cctcgtctct tcgggaagcg cctgggtcat cggcgtgtcg atgtagccgg gagcgatggc
     9841 gttgacgcgc acgccgcgcg cgccgagctc cttggcgagc gacttggtga ggccgatgat
     9901 ccccgccttc gccgccgcgt agttggcctg gcccgcgttg ccgcgccggc cgacgacgct
     9961 cgaaagattg atgatacagc cggtgcggcg gcggaacatc ctgcgagccg ccgccttgct
    10021 cacgaggaag gtcccgcgca ggtgggtcgc gatgacggcg tcgaagtcgg ccgggtccat
    10081 catcatcgtg acgttgtcgc gcgtgatgcc ggcgttgttg acgaccacgg agagcgggcc
    10141 caactcggtc tcggcccgct cgaccatctc gttgacctgc ccctcatcgg agacgtcgcc
    10201 catgacgacg aggcccttgc cgccgagcgc ctcgacctcg gcgacgacgg ccgcgcaggc
    10261 cgcctcgtcg gcgaagtcgt tgacggccac gtcggcgccg gcgccggcga gcgcgagcgc
    10321 gatgccgcga ccgatgcccc ggccggcgcc ggtgacgagc gcggtcgcgc cggtcaggtc
    10381 cagtcctgca gtgagcggtg tcacgcgtcc tccagatagc ggccgtctgc gcgcagcgcc
    10441 agcagcgtct ccatatcgga gactcgagcg gcttgcgcct ggggctcgat gcgtttgacg
    10501 aggccggtga gcacggcgcc cgggccgacc tccaggaagg catcataacc tgctgcgtac
    10561 agagtgctca cggattgcga gaagcggacc ggcgagacca gctgcacctg cagcagctcg
    10621 ttgaagtcgc cggtctcgaa ggccacgctg caggcggaga agaacgcagg ctccggcgtg
    10681 cgccactcgg tgcgctcgaa ctcatcgcgc agcggttcga gcgccgactg cacgagcggt
    10741 gagtggaagg cgccgctcac ggcgagcgag atgacccgct tggcgccggc ggccttggcc
    10801 ttctcggaca ggacctcgag ggcgggcttc gtgccactga tcacgacctg gccggggcag
    10861 ttgaagttcg ccggccagag gccgtcctgc tcggcgcaga gcgcctcgac gacggcgtca
    10921 tccagcccga tgaccgccgc catcccgccg ggactggcgt ggcccgcacg cagcatctcc
    10981 tggccgcggc gcctcacgat gcgcagcgcg tcggcgaagt cgaccgcgcc cgtcgccacc
    11041 agcgccgaga actcgcccag gctgtgcccg atcgccacgt cgaactcgag gccctcccgg
    11101 cgcaggatgc ggaacatgcc cactgacgcc gcaagcaacg ccggctgcgt gaagtcggta
    11161 cggctcagcc tgtcgatggg cccctggcag cagaggtcct ggaggtcgta gcccagcacc
    11221 tcggaggcct ccgcgtacgc gtcgcgcatc gcggggtcgg cggcggcgag gtcgcagccc
    11281 atgccgacgg cctgcgaccc ctggccgggg aacatgacga cgaggttctt cacggggcta
    11341 gattacagct cccacttggt gagacacgag ccccaggtga gtccggcgcc gaagccggcc
    11401 aggagcagcg tgtcgccctt cttgaggcgg ccgctctcgc tggcctcgtg caggcagatc
    11461 gggatggacg cgcacgacgt gttgccgtag cggtcgaggt tggcgaacac ctggccctcc
    11521 ctcatcccca gccggcgcga cgccgactcg atgatgcgga tgttggcctg gtgcggaacg
    11581 aacagatcca catcgtcgac cgtgatctcg gcgtccgaga ggaccttctg gcacgagtcg
    11641 acgacggtgc gggtggcgaa cttgaacacc tcgctgccgg tcatgtgcat ggcgtgctcg
    11701 tgatcgcgaa cggtgtcgta ggtagccggc atgcggcttc ccccggccgg caccagcagg
    11761 ttgggtgcgc cgctgccgtc gttgcccagg tcgaagctga agatgctgcc gttgtccgtg
    11821 ggctgcacga gcacggcgcc ggcgccgtcg ccgaagagga cgcaggtgct gcggtcctcc
    11881 cagtccacca tcttgctgag gacctcggcg ccgacgacga gcacgttggt gtagatgttc
    11941 gcggagacga acgcggtcgc gatggcgaga ccgtagacga acccggtgca ccccgcctgg
    12001 acgtcgaacg cgccggcctt gccggcgccg atcttgtcgg cgacgatcga cgcggtcgcc
    12061 ggcatgatgt ggtccggcga gatggtcgtg acgatgacga ggtcgagctc ggccgcgtcg
    12121 aggccgccgg actcgaggca acggcgggcg gcgacctcac ccaggtcgct cgccgcctcg
    12181 tccacgcgcg cgatgcgccg ctcgcggatg cccgtgcgcg tggtgatcca ctcgtcggac
    12241 gtatcgagga actggctgat ctcgtcgttg gtgaggacgc gctcggggac gtgcacaccg
    12301 agcgcgctga tgcgggcgcc tctcacgaga ccgcggcgca gaccgatcac agcttggcct
    12361 ctccagccgc gacggacgcc gcgtcgacga tcgcgaacat gagctcgccg cggcaggcca
    12421 gctggccgtc cacggtcgcc ttgaccttgg cccggcccac cggaccgtga acggcgacga
    12481 tctccgcctc gagggtcagg gtgtcgccgg gcttgcagac gcgcttgaag cggaccttgt
    12541 cgatgccggc gaagaggccg accttgtcgc cgccgggggc ggcgtccgcg gacggcagcg
    12601 tgtcgccgcc cgccgcggct tcggagcctg cgctcgccgc ggcgatggtc gccgtctgcg
    12661 cgagcgcctc gacgatgagc accccgggca tgaccgggtt gccggggaag tgcccggcga
    12721 accaccagtc gtcgtcccgg agcgtgcgga ccgccacgca gcgcgccccc ggctccccgt
    12781 cgacgacctc gtcgaccagc aggaacggcg ggcggtgcgg cagcagcgtc agcacctctt
    12841 cgttggtcag tctcacagcg gcttcaactc cctggtacgg cgcaggtagc gctcgacctg
    12901 gatcaggacg gcggtcgcgg cgatgccgta accgaggacg ttcttgacgg ggacggtgcg
    12961 gccgccgggc agcgggaggc ggccgagggt cgcgtcgagc gtgtcgagca cgtcgccgac
    13021 gacgcgcccg agcgtggcct cgcccggcct ggcctcgagg gcggcggcgg ccgcgacgcg
    13081 ctccgcgaac cccggcggag gcgcgagctc gacctctccc cagacggcca tggcgccgcg
    13141 cacctcgctg agctgggaga gctccgcggc gcagtgcggg cagaagctga ggtggctctg
    13201 gaactcggcg acgtcgaggt agccgagctc gccgtccagg aactgattga ggcgcgagcg
    13261 gaagtagctg cagtccatca ggcctcccgc tccgcacgca ggcgcaggcg cagggacgcg
    13321 cgcccgcggt gcaccatgac cttcacggtg cccaacgggc gcgccgtgag atcggcgatc
    13381 tcctggtacg gcagtccgta gatgtcccgc agcacggtcg gcacgcggta gtcgtcgggc
    13441 aggtcccgga gggcggcggt cagggtggcg gcgctgatgc cgtcgccgag ctcggcctgc
    13501 cccccgtcgg cgacatccgg cacctcgacg ggctccggcc ggcgtcgctc gcggcgctgg
    13561 tagtcgaccg acgcggtgag cgccagccgg aacagccagg tgctgaacgt cgcctcgccg
    13621 cgatactgcg ccagcttgcg gtagcagcgc aggaacgcct cctgcgccgc gtcttccgcg
    13681 tcggtgtggc tgccgagcac gttgtaggcc accgagtaga ccatgccctg ataccgctcc
    13741 acgatcgtct tgaaggcgtc cacgtcgccc gccgcggcac gcgccaggtc ggcgggcgtc
    13801 gggtcgggca gcatcgccca cttgtggagg ttagacgtcg acatgcggca gggagttaca
    13861 acctggatgg ggggcggatc cgggagcacc agtgtacacc cctgatgacg gacgcctcgg
    13921 cagcccgcac ggaggcctct cttccacgcg agctctaccg gcggtcggga gccgcgctcg
    13981 acgcgagccg cctgcgggtg gcgtcacacc ccggcggccc tgtcggcgag ggccgcctcg
    14041 aggagcgcgt ccaggcgggc gtcttgtgcg gcggtgagcg agaaggcccg cccggcggcg
    14101 cggaggccgg cgaggcgctc gcgcacacgg gcgccgagcg tcgtggcgcc ctcggcggcc
    14161 cagcggtcgt ggacggcgtg gtcgagaagc gccggggtcc agaactcgcg gtggtgccgg
    14221 cgcgtgtact tcgtcccgag gtggctgccg ccgggaccca ccgccttgat ctcgtcgagc
    14281 gccaggctgt agtcgtccac cggcacctcg cgcatgaact ggcgcgcgta cccgaccagc
    14341 tcgtccccga gcacgatgga ctcgtagctg ctctgcagcc cggactcgag gtagccgacg
    14401 tcgtgcagca gtgtggcccg
//
LOCUS       DASUMX010000063        29506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_17347, whole genome shotgun sequence.
ACCESSION   DASUMX010000063 DASUMX010000000
VERSION     DASUMX010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 29506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29506
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17347"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="VFZ86_02535"
     CDS             <1..595
                     /locus_tag="VFZ86_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641197.1"
                     /translation="MDHVKRLRPSPAMIVAIVALVLSLGGTSYAAIVLPAGSVGTTQL
                     KKNAVVGSKVKNGSLTVADISAASLSKLGRVAIASGHVNVASNASSRVVQVSITAPSK
                     GFVLVNGWVNTDGASGQYTVRVWDDGASTHSPYLNGDYVTAGESTCGNAGVFPVAAGV
                     QKFSVRIESNTAGDMEAWGTITAQFIPYNGIGTQTAP"
     gene            complement(969..1349)
                     /locus_tag="VFZ86_02540"
     CDS             complement(969..1349)
                     /locus_tag="VFZ86_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HEX5641198.1"
                     /translation="MEYKSMIRVRMSSHDAHYGGDLVDGARMLELFGDIATELLIRMD
                     GDEGLFAGYERITFKAPVHVGDYIEATGEIVSVGNRSRKMSFVAQRVIAGRPDVSASA
                     ADFLEEPVLVCTARGTCVRGPERS"
     gene            complement(1784..4036)
                     /locus_tag="VFZ86_02545"
     CDS             complement(1784..4036)
                     /locus_tag="VFZ86_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="immune inhibitor A domain-containing protein"
                     /protein_id="HEX5641199.1"
                     /translation="MSSKLFRFTLLTILMLLLAAPVVWAAAVANSNGASDARNRQVRR
                     DDRHSPLADKQAALKQVALEKKLQGKLTGKDKNGVVEVAEGQFVKLELEGEDPVWTML
                     GEFADFPHNNIAEPNRAYDNSTIWVQDFSRDYFNELLYDDSSGANSMRNYFLEQSSGR
                     YGVYGDATDWVTVPEGYLYYDDSVGGEDTSTNVWLLLQDMADGWYEDQIAAGRTPAEI
                     DAYLAQFDLLDRYDYDGDGEFKEPDGYIDHFQALHAGEGEEAGGGALGADAIWSHSWY
                     ANYADIDKTGPSPDYLLGGLKIGDTNYWIGDYTIQPENGGVGVFTHEFSHDLGVPDLY
                     DYNYLENSTGFWTLMSSGSWLSDGAYDIGSKPGHLGAWEKFLLGWLDYDVAIAGDTAQ
                     TVLGPSEYNSSEKQALFVVLPEYQVTEVIGAPYAGTMFYFSGSANNLNNKMTKAFTLG
                     AGSTLAAKVNYGIEEGYDFANVIASTDGGATWRTVPTNLSNSSVEANGIEGFSGGWVD
                     LTADLSTFTGDVMLGFQYRSDGGVNYDGFMVDDIQITGQALDGAETDAGWTYDGFRAT
                     TGTEEKTYTHYYIAENKVYWGYDYTLKVGPYNFGFLNNPNLQNYVERFPYQDGMLVSY
                     WNTRYANNDTDPSRPGTGMLLYVDAHPQALKRPDGAVWRNRVQTFDSTFSLARTDAIT
                     LHYNSKAATYKSLPAVRTFNDLTTYYDMKNPYGSVKLPKTGTKIRIDGVSSSGRYLQI
                     TVSPSKSPAR"
     gene            complement(4182..4904)
                     /locus_tag="VFZ86_02550"
     CDS             complement(4182..4904)
                     /locus_tag="VFZ86_02550"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013376564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5641200.1"
                     /translation="MQGLAGKKVVITGGTSGIGAATAARFREEGCEVVILARTPGPDV
                     IPCDVGDKRQVEQAFAQIGSLDVLIANAGVSIRSPALDIDAGQWDQVVATNLSGAFWA
                     AQAAARSMLAGGGGAILFTASTNALVGYRYYADYNATKAGLVALTKSLALEWAPTVRV
                     NAVCPGYVLTPMQEAEYTPEMLEQVNRRIPLGRHATPEELAGLFAYLAGDEAAYFTGS
                     VLVMDGGETAGSVVSGPGGPGA"
     gene            4951..5433
                     /locus_tag="VFZ86_02555"
     CDS             4951..5433
                     /locus_tag="VFZ86_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015319209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX5641201.1"
                     /translation="MTRPPKPFIGVLSDTHGYYDAALDDLLAGAEHIVHAGDIGTLEV
                     LSRLRAIAPLTAVLGNTDLPFWEGDLPWEAEVVVLGRRILVGHIGKSLMGRHDPVAEG
                     FDLVVSGHSHKAAVVWRESTLFLNPGAAGKARFGQPRTLALVTIDADGQLAPEIVPLD
                     "
     gene            complement(5485..6978)
                     /locus_tag="VFZ86_02560"
     CDS             complement(5485..6978)
                     /locus_tag="VFZ86_02560"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HEX5641202.1"
                     /translation="MLWLRRDLRLGDNPALLAAARIAEDGGGSLLPVYVWEPGERRAW
                     APGGAARWWLWHSLASLDRGLGRLGSRLLVERGDPVAAVAALAERAAAEAVVWARGLE
                     PDETADDDTLEAALTARGIGAVVAPQANLLADPLSVRTRDGRPYTVFTPFWRARLAAG
                     APEEPLPAPRALPPAPADPRGLALSALEAEALRPWAAGFGDVWRPGEAGAHARLEAFL
                     EGALAGYASGRDAPGTEGTSRLSPHLRWGEVTARQVWHAVSGTLAEAGLEVEAALGPT
                     NWDAEQAPGLRRSAGAFLRQLGWREFGHHLLAAFPHTTTQPLHARFAAFPWREDPAAL
                     EAWRRGRTGYPIVDAGMRELWTTGWLHNRARLLAGSFLVKDLLLPWQSGAAWFWDTLV
                     DADLADNTLGWQWVAGCGADAAPYFRIFNPVTQGRRYDPDGAYVRRWAPELGGLDARD
                     IHAPWTAPEGALPAAGVSLGETYPEPLLDHGEARSRALAAYEAVRAR"
     gene            7126..8019
                     /locus_tag="VFZ86_02565"
     CDS             7126..8019
                     /locus_tag="VFZ86_02565"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5641203.1"
                     /translation="MSGGTTPEHQAALRRLREGWGRMDGRVVLVTGATRGIGLETAVR
                     LAGLGADVLVHGRDPGRGAAALAAVRAASTPAAAPALYLADLGAPADVRRLAAEVTRD
                     RPRLDVLLANAGVYAPERRVSAGGLELTLAVNVVAPMLLACSLLPVLRAAAPARVVVL
                     SSASHWTGEIHWDDLQLAAPGAYDGLRAYDQSKLAVLMLTLALARRLRGSGVTAVSLD
                     PGDVATTLLASGWPGLPGISVENGAVTSVYLASAPDAAGVSGAYFEDGAAVMPLAAAL
                     DVAAQGRLWGLVEDLAGPFSV"
     gene            8088..8345
                     /locus_tag="VFZ86_02570"
     CDS             8088..8345
                     /locus_tag="VFZ86_02570"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HEX5641204.1"
                     /translation="MTRTPPVTIYSQTTCGWSRKAKEYFKQRGIKAFVIEYDTAGPAL
                     QQKISAEMRKEGADGFPFVRIGGRVVKGYDPATYDRLLRGA"
     gene            8381..8704
                     /locus_tag="VFZ86_02575"
     CDS             8381..8704
                     /locus_tag="VFZ86_02575"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEX5641205.1"
                     /translation="MAEQTKVELYTLSTCPWSRNTRAWLDKRHVAYDFVDYDLADQGL
                     QAQIQEEMVRRGASAFPFVRFGETDFIIGYNPDAFARLLGLPTDAELDAAKGEEGPAK
                     GGGAG"
     gene            complement(8708..8784)
                     /locus_tag="VFZ86_02580"
     tRNA            complement(8708..8784)
                     /locus_tag="VFZ86_02580"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8748..8750),aa:Pro,seq:cgg)
     gene            8980..10668
                     /locus_tag="VFZ86_02585"
     CDS             8980..10668
                     /locus_tag="VFZ86_02585"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX5641206.1"
                     /translation="MSLARKGVLLAIVVLLVLLAAAGAALAAKPTKVVIITMDQMKPW
                     YASAFDMDNILWLQSRGANFKNATVGQMASETVVSHNTIVSGQFPKHMGWTDEVMRDT
                     SDVLGYGAGAIVTVGDLTYEQFTLLVEAEGYPKLGDYMHAKFPGKVVANFGGKYYQVA
                     STAASSSDIWVTFGSKAQTSALPVPEALPWSGTYRGPSGGNVPAYIADDDRFKISTGN
                     DFSTASPSPAPNDYYGTKTDKPAYLYPEDGRMVPGPYETNLGGDDWVADAAVKVIENE
                     DWSALHLNFSGIDKIGHMWGGGNVDKVSRYDWDPATIMAQVHMPWIAKNADDQVGKVM
                     RALKAEGDWSSTLFIVLADHGSTWAKRAHYVDAAGGGNLSWYYDPNEVAKNTTYGRFG
                     ANNEAVLAPLNATGNLAYSYQSTAIEAWLIDRSWTKKAEAAAAVKAMPDVIATYVRWG
                     DRYVLTSRGSMTRAERIWWEGHGQRLVNTMASPTGPDVVGLLKDQTGYGVYGDHGGAQ
                     REVQRVPMVMYVKGMKPKVSTAPFRLVDVLPTVLYNMGIKKMAPMDGRAYSLGL"
     gene            10906..12639
                     /locus_tag="VFZ86_02590"
     CDS             10906..12639
                     /locus_tag="VFZ86_02590"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX5641207.1"
                     /translation="MGTARKSLLIALVVLLILLVAASTAFAKAPTRVMIVVMDQMRPE
                     YAQQYDMTNVLWLQNRGVNFKNAYVGDMASETVVSHNVMVSGLFPKHMGWSDEAIRDV
                     DNVLGYGAGKIVTVGDLGYADYVKLITAAGDYPKLGDYLHARFPGTKVCNVGQKGYQV
                     ESMAALSSDYWVRMGSKKNTADLTGVFSPASVPWVGTYRGVGGNAPDYIKNDPRYLVS
                     VGNNRPDTDPAWPADYYGTRTDRPAWLYPEDGRYVPSPYTDPAAYHESGDAWVADAAI
                     EMMENEDWSGMFVTLSAMDKIGHMWGAGSVDDLQHYTWDPSSMFDWVHMPFVAKNADD
                     QLGRLIDTLKANDQFDETLIVVTADHGGQDSINGYYGHDKYDGGNSSWYAGTWLPGYG
                     SDPTVPNTTAGPDALQPLMATGNVDFSYQSTAIESWLKDASAAKKAQAANIMGTLPGV
                     VATYKKSADSTRYVLHSARTSTRMNPSELAWWQLHGQELVDTMAFEGSADVVGLLKDR
                     VGYGVFGDHGGAQKQVQRIPMIVYNPSIPRAVSGNGIRLADLMPTVLRTMGIPLTAPV
                     DGRAYKLPVLP"
     gene            complement(12931..14019)
                     /gene="hydE"
                     /locus_tag="VFZ86_02595"
     CDS             complement(12931..14019)
                     /gene="hydE"
                     /locus_tag="VFZ86_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HEX5641208.1"
                     /translation="MSRPAAPPLGVADALTRDAIEWWLRETDEARLETLWAAADEARR
                     RYVGDAVHLRGLVEVSNYCVRGCTYCGIRAGNRGIQRYRVSEDVIVDCARKAVAFGYG
                     TLVMQAGEDYGITAEWMAGVLRRIRAETTVWAITLSLGERPDEDLALWREAGADRYLL
                     RFETSDEQLYRHIHPDLPGKVSDRMRILRRLQELGYEAGTGIMVGIPGQTHASVAGDI
                     ELFRGMDMDMIGIGPYLPHPATPLGREFERRLAEDDWPPDQAPNTELMTCKVVALTRL
                     ARPDANLPATTALSLVNKAGGRAHGLQRGANVVMPNLTPREERERYEIYPEKAAVHET
                     AEAINESIQKLLSSLGRTAGAGAGGRGG"
     gene            complement(14021..15319)
                     /gene="hydF"
                     /locus_tag="VFZ86_02600"
     CDS             complement(14021..15319)
                     /gene="hydF"
                     /locus_tag="VFZ86_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HEX5641209.1"
                     /translation="MEAPKGMRLHIGIFGRRNVGKSSLLNAITRQEVSIVSDFAGTTT
                     DPVEKPMELLPLGPVLFIDTAGIDDVGVLGELRARKTTQVFDRTDLGVLVTEAGSWGE
                     YEDGILDEFLERKTPIVVVFNKSDLIDRPAPVKRLEDLKIKPVLTAASAGRGVLDFRQ
                     ALLDAAPPDFIDNPSILGDLVNSGDLAVLVVPIDKEAPRGRLILPQVQSIRDLMDSDA
                     MALVVKERELRNAIERLNRPPRLVVTDSQAFLKVAADTPPEVPMTSFSILFSRFKGDL
                     VSQVEGTLAVDDLKSGARILIAEACSHHPIAEDIGRVKIPRWLTQYVGGRLDIEHMQG
                     HDFPDDLSRWDLVIHCGACTFNRRAMLGRILRCRQQGVPITNYGLVIAFSLGILERAL
                     QPFPAALEALRAAQARRAGHAPAEPVTAGLPDGAAVDDLC"
     gene            complement(15344..16774)
                     /locus_tag="VFZ86_02605"
     CDS             complement(15344..16774)
                     /locus_tag="VFZ86_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014413850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HEX5641210.1"
                     /translation="MTDDVRIERDLLGEVAVPAGSLHGAHTERALENFPLAGRPVHVE
                     LARAFGVVKLACARTNRALGVWGEDEPKADAIERACRELGDGLLDEHIVADRLQGGAG
                     TSTNMNVNEVLANRALQLLGAAPGDYARVSPSDDLNLHQSTNDTYPTALRLAAIELLH
                     RLEEQIVSLQEAFQAKEQEFAHVVKVGRTQYQDAVLTTLGREMGAYAEALNRDRWRIY
                     KCEERLRVVNLGGTAIGTGFAAPRQYIFRVVDVLRELTSIGFARAENLTEATQNADVF
                     VEVSGILKAHAATLLKISGDLRLLSSGPEAGLGEIRLPRRQAGSSIMPGKVNPVIPEA
                     VSQAALLVFGYDATIATAAGLGSLELNAFLPLVADCLLESLRLLAGADDVLRRLCVEG
                     IEADEERCAAHVRASSAAATALVPEIGYDAACDVARRARERGASIRDVVVGDGVLTAA
                     QYEELLTAEAVCRLGTPQTRGRGVED"
     gene            complement(16794..17054)
                     /locus_tag="VFZ86_02610"
     CDS             complement(16794..17054)
                     /locus_tag="VFZ86_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005032591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641211.1"
                     /translation="MTDDHIILGIHVTDRLQEAGAVQAVFTEFGCNIKTRIGLHDVDE
                     SFCSPSGVVLIEFFGSESEAVAMAEKLNAVEGVHVQKMVFGH"
     gene            complement(17104..18507)
                     /gene="hydG"
                     /locus_tag="VFZ86_02615"
     CDS             complement(17104..18507)
                     /gene="hydG"
                     /locus_tag="VFZ86_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008685260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HEX5641212.1"
                     /translation="MTAVVETGAFIDEAAIAQAMQASATKDAVRVREILAKASELDGL
                     DMDEVAVLSSVSDPELLAELFTTARECKEAIYGQRLVLFAPLYISNLCNNDCLYCAFR
                     VRNKELKRRALTQQEIRHEVELLVDQGHKRILLVAGESYPKDGGFQYVLDSIGTIYDV
                     KRGNGEIRRVNVNVAPLPVDQFRELKASGIGTFQLFQETYHRETYRAVHIGGKKRDFD
                     WRVTGMHRAMEAGIDDVGIGVLFGLADWRYEILAMLQHIHELERAYGVGPHTISVPRL
                     EPASGSDMASHPPQPVGDIDFRKIVAILRLAVPYTGMILSTRETANLRQETFALGISQ
                     ISAGSRTNPGGYEEDEEFDAAQFQLGDHRSLDEVIRDISELGFIPSFCTACYRLGRTG
                     MDFMDLAKPGDIRHHCDPNALSTYLEYLIDYGSQQTRAVGEQVIEQKVAEMDPVRRAH
                     TEKMLAQVRAGNRDVLC"
     gene            complement(18511..19869)
                     /locus_tag="VFZ86_02620"
     CDS             complement(18511..19869)
                     /locus_tag="VFZ86_02620"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEX5641213.1"
                     /translation="MAKEPQQTQRPAVTYVDPFRDGAALKPIGAEARTLLRTVNQKIA
                     ARPSLRAIVDYLFDNTQVLIPCDRMGLAFVDEKGEYVTSYYNRAAYEKLYVKQGYRES
                     LLGSSLLEVLRGGLPRIIGDLEAYGHEHPRSRSTRLLVREGVRSSLTCPLSVEGRVVG
                     FIFRSSRAPHAYGPQHIELQMAITERLSQAVEKAWRIEQLEEANYAYTQMLGFVSHEL
                     KSPVASMVTDAQILAQGYLGDLTPEQRAKVESMARKGQYLLSLVREYLDLARVEGGEL
                     KLATRSRVDVMNEVLEEAVDLVRPQSDAHGMHLMVCMPSPALPPVCCDPTLLRIVTVN
                     LLDNAIKYGNEGGDIRVKAEVLVDRKHGERLRISVWNSGPGFSQSQQDKLFRRFSRLD
                     DPALKSRRGTGVGLYNSWRIVQLHGGRITADSKHGEWAEFSFEIPATADCAEPSAVDP
                     RE"
     gene            complement(19946..21874)
                     /locus_tag="VFZ86_02625"
     CDS             complement(19946..21874)
                     /locus_tag="VFZ86_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005034114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HEX5641214.1"
                     /translation="MSNPTTPIHRAPLSQQHRRVITTTSPDAIGAQVSVTIDGVDAKV
                     PFGVTILEAARNAGVKIPTLCDHPDLDVAGVCRMCVVEVEGQRTLQPACTFPVVNAMK
                     VNTHSRKVRMARKHVLELLLSEHCGECYSCVRNGNCELQSLAEEYGITAFPFGHPEEP
                     LFEQDTSSYSVMRDMNKCIRCRRCIRTCIDIQEVGVLEAVGRSNKVTIETFEDMPLGT
                     AVCINCGQCVNRCPVGALYAKDETDAVWAAIDDPDRHVVIQTAPAPRSAMGELFGLEP
                     GTPVTFEMNTALRRAGFDKVFDTNFTADLTIIEEGTELILRLYKALVEKDESVALPQF
                     TSCSPGWVKYLEHFYPEYLPNASSAKSPQQMFGALIKTYYADKLGLDPEKIVSVALMP
                     CSAKKFECNRPEMRDSGFQDVDYGLTTRELGKMFKEAGLHVPSLPKSDFDDPFGSATG
                     SGVIFGATGGVMEAALRTVIEMVTGEKIESIYDHGDIIPVRGFEGVKYAEITVPKVGP
                     VPEIVAHLVPDWDWLDGATLKVAVAHGTANAKKVMDDIKAGGKFSECHFIEFMACPGG
                     CLGGGGQPIPTSADIRAARAKAIYSEDSAYAVRKSHENPAVLATYGEFLTDGPCGHKS
                     HKLLHTEYTARGKHMPQS"
     gene            complement(21894..23090)
                     /locus_tag="VFZ86_02630"
     CDS             complement(21894..23090)
                     /locus_tag="VFZ86_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEX5641215.1"
                     /translation="MPATIETQGNALTYETIEPEAGLKAALAMSRADLVDFVRDSDLK
                     GRGGAGFPTGMKWGFCAAEKADQKYIICNADEGEPGTFKDRVILTRFADLVIEGMTIG
                     GRAIGATLGIIYLRAEYTYLRPHLNEVIQKRRGQGLLGHDIMGVEGFDFDILVAMGAG
                     AYVCGEETALIESLEGFRGEPRNRPPFPVVAGLLNNPTVVNNVETLASVAAIFAKGCD
                     WFKTFGTDKSSGYKLFSISGDCEKPGVYEFPWGITVASLLEEVGGTGAKAVQIGGASG
                     VCIPAKDFTRALAFEDVPTGGSVMVIGPDRDILDVAENFLDFFVEESCGQCTPCRLGN
                     AQMLRAVKLLKTGECPPEFLASVKALGATMQVASKCGLGQTSPSAFLSILEGFDDEIA
                     NRAVSA"
     gene            complement(23094..23585)
                     /locus_tag="VFZ86_02635"
     CDS             complement(23094..23585)
                     /locus_tag="VFZ86_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005034123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEX5641216.1"
                     /translation="MTDQDQLRADVEALVEKLGPGRSSLIPILADVKRRFHEVDSESM
                     QTIADVLDIHPVEVYSVASFYAFLHGAPTGQFVIRLCGTLSCDFAGKDAVADALTDAL
                     GVGFDQTTDDGLFTLEWASCVGMCDQGPALLVNDEVHTRVTPDAAREIVAACRAAAVA
                     KEG"
     gene            complement(23611..23982)
                     /locus_tag="VFZ86_02640"
     CDS             complement(23611..23982)
                     /locus_tag="VFZ86_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005034124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5641217.1"
                     /translation="MPKILIVDDDPDIVDAGRLVLEREGYEVVGAPNRADGMKKLEEI
                     KPDLLILDVMMEEPDDGLRMAREIRKNGNEVPIIMLTSVNAAMGLNIDKDGEIVPVDE
                     FQPKPVEPQTLVAKVKKLLEQ"
     gene            complement(24295..25521)
                     /locus_tag="VFZ86_02645"
     CDS             complement(24295..25521)
                     /locus_tag="VFZ86_02645"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5641218.1"
                     /translation="MNPDPTARESLMDPQQATLKVVMVDDEEALCLGVRRIIQKYRVH
                     VADVLVDADYEFRYFTSGEDYLAWLGEGGEVDLLLLDLKLPGVGGMDVLDAMSAQGRE
                     VLTIMITAYATFETAVKATKLGAYDFLAKPFTPEELRYALRKATSQLILSKQARKLAE
                     EKREIRFNFISVLAHELKAPLNAVEGYLNILRTTEADQNLQMVERSIVRVDGMKKLIM
                     DLLDLTRIESGQRERVIKRVDLRELAKASMELFAVDAERRGITVELEDGADIELLADP
                     GEVEIVFNNLISNAVKYNRDGGSVKVRLSRRGGAVTVTVSDTGIGLSAEEAGKLFNEF
                     TRIKNQQTVKILGSGLGLSTVRKIANMYDGEAKVSSEPGVGSTFTVTLHDAPPAGAAP
                     AREPAADAAVPAAPQL"
     gene            complement(25526..27580)
                     /locus_tag="VFZ86_02650"
     CDS             complement(25526..27580)
                     /locus_tag="VFZ86_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HEX5641219.1"
                     /translation="MNEETVPYLQLVTTIREKCRRCYTCVRECPAKAIQIEDGQASVI
                     QTRCIGCGNCVTVCSQNAKQVLSGIEAAEELLAGDAPVAAIVAPSYPAEFTECGTGGL
                     VGALRELGFASVHEVSFGADLVAAEYARLLEQDDGRHIATTCPAVVSYVRKYHPDILD
                     RLAPIVSPMLAIARVLHKKHGPDLRIVFIGPCIAKKGEARYEQFEGEVHAALTYVELR
                     TLLAARGVGLTSRDRAGDDFDPPHGSLGALFPITRGMLQAADLDEDLLSGDIVSADGR
                     QNLVEAITDFEHGDTEARLLDILCCEGCIMGAGFSCEEPVFKRRSDVSAETRARQAAF
                     DQAAWEAAVAEFADVDLSRTFAPFDQRFDDMPSGTEVEEILARMNKHTDADELDCGAC
                     GYDTCRHHAVAIWQGLAEVEMCLPYSVEELRKTVDELQQSNLSLETTKEALVKSEKLA
                     SMGQLAAGIAHEVNNPLGILLLHANLLLEDCEDDPDVSADVKLIVDQANRCKKIISGL
                     LNFARQSRVVRQPTDLTHLVAEVLRTIPVAEGVSVEVVDDLEDPIAELDGDQIVQVLT
                     NLLTNAQHAMPDGGTIVVTVDGTPENVLLCVRDEGTGIPAENIGKLFSPFFTTKQVGK
                     GTGLGLAVTHGIVKMHRGQITVESNADPAAGPTGTTFCISLPRYEAETDVPASLQEIT
                     GS"
     gene            complement(27600..28010)
                     /locus_tag="VFZ86_02655"
     CDS             complement(27600..28010)
                     /locus_tag="VFZ86_02655"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5641220.1"
                     /translation="MSAEHKQQILIVDDDFDFLEINRFILEGAGYRVVTATNPAEAQA
                     RIDAEAPDLVITDLMMTSIDAGFTFAAALKSDPSTADVPVIISTSVSSALGLNFRPDS
                     AEDLAKMNVDAYFDKPIDAQALLAKVGELLAARG"
     gene            complement(28183..>29506)
                     /locus_tag="VFZ86_02660"
     CDS             complement(28183..>29506)
                     /locus_tag="VFZ86_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016695 - oxidoreductase activity, acting
                     on hydrogen as donor [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="[FeFe] hydrogenase, group A"
                     /protein_id="HEX5641221.1"
                     /translation="RCVTVCNEVQHVGGLFAQGRGFATLIGPAFAQDLSEVVCVQCGQ
                     CAAVCPVGAITERNQIDQVWDALEDPTKTVVVQTAPAIRAALGECFDYAPGTLVTGKM
                     VTALRRMGFDAVFDTDFTADLTILEEGTELLVRLKKALVDGEEVALPQFSSCSPGWIN
                     YMEHFNPDMLANLSTCKSPQQMFGAVAKTYYAEKIGVKPEDMVVVSVMPCTAKKYEAA
                     RPEMNDSGVRDVDIVLTTRELGAFIKQAGIDFRSLPDEKMDAPLGISTGAADIFANTG
                     GVMEAALRTAWEIVTETPFPFPNLHVAPIEGLEGVKVASVKVEGAKGDWAFLEGADLK
                     VAVAHGLGNAQKVIDQVKNGEAEYHFVEVMTCPGGCIGGGGQPRFTTNEVRLKRIEAI
                     YKEDEGRELRKSHENPAVCALYEEYLGAPCGEISHRLLHTHYFEHDRV"
BASE COUNT         4239 a         9885 c        10582 g         4800 t
ORIGIN      
        1 aatggatcac gtcaagcgcc ttcgtccttc accggcgatg atcgtcgcga tcgtcgccct
       61 cgtgctgtct ctcggcggca ccagctacgc ggcgatcgtc ctgcccgccg gcagcgtcgg
      121 cacaacgcag ctcaagaaga acgccgtcgt cggctccaag gtcaagaacg gatcgctcac
      181 cgtcgcggac atcagcgccg ccagcctctc taagctgggc agggtcgcga tcgcatcggg
      241 tcacgtgaac gtggcgagca atgccagctc ccgggtcgtg caggtctcca tcacggcccc
      301 gagcaagggc ttcgtgctcg tcaacggctg ggtcaacacc gacggggcca gcggccagta
      361 caccgtgagg gtgtgggacg acggggcgtc gacgcactcg ccgtacctga atggcgacta
      421 cgtcaccgcg ggcgagtcga catgcggcaa cgcgggagtg ttccccgtgg cggccggggt
      481 gcagaagttc tccgtgagga tcgagagcaa taccgccggc gacatggagg cctggggcac
      541 gatcaccgcg cagttcatcc cctacaacgg gatcggcacc cagacggccc cgtgagctgg
      601 gagtcgtcgg tggcctcgcg accgccggcg tgacgcgcgg cgacgcggct ggaccttcgc
      661 gtagcgctgg tcggcgaacg agagaagggc gccgctcccc gccggggcgg cgcccttctt
      721 cgtccggggt ggccgcgcga cgctgcgtcc cgcggcctct caagctccgg tctctcgctc
      781 ctcgctcacg ctactgccgt ctcagggcca ggggacggcc cgggcccgca ggggagtcag
      841 ggaggggacc aatggatcac gtcaagcgcc tgcctccctg cgccggcgat gatcgtcgcg
      901 atcgtcgcac tcgtcttgtc gctcggcggc accagctacg cggcgaccta ccgccgctac
      961 catctctctc atgaccgttc gggtccgcgc acgcaggttc cgcgcgccgt gcagacgagc
     1021 accggctcct cgaggaagtc ggcggccgac gcgctgacgt ccgggcgccc ggcgatcacc
     1081 ctctgcgcaa cgaacgacat cttgcgcgag cgattgccga cgctcacgat ctcgccggtc
     1141 gcctcgatgt agtcgccgac atggaccggc gccttgaacg tgatgcgttc gtagccggcg
     1201 aagaggccct cgtcgccgtc catccggatc agcagttcgg tcgcgatgtc gccgaagagc
     1261 tcgagcatcc gggccccgtc cacgagatcg cccccgtagt gggcgtcatg ggagctcatc
     1321 ctcacgcgta tcatcgactt gtactccatc gtggctgcca cctttcgcgt gcgcggggaa
     1381 tcggctgacg ccattctatg ggaggctcgc cgcggggccg gccggcgcta tccactcagg
     1441 acgcccccga gccagatgat gaggaacacc agcagcgcga gcgcaaggac gatgacgatg
     1501 attcggagcg ggttcacaac agcctcctgg cgtcgcggga ttcgctacca gcccgtgcct
     1561 cgtcggggcg tgggcatcgg cccacgtatc tgattccctc cgcggccggc cacgactgca
     1621 gtgccgttgg gactgtgact tcgattcgga gggccctccc cgaaaggacg gcggggagaa
     1681 cccgttggcc ctccgctcgc acgtggtagg gccggtggaa agaggaaggg cgccccttgg
     1741 ggcgcccttc ctcgcttcac tcagcgccgt ggtggcgcgg cgactacctc gcagggctct
     1801 tggaagggct caccgtgatc tgcaggtagc ggccactcga gctcactccg tcgatcctga
     1861 tcttcgtccc cgtcttcgga agcttgacgc tgccgtaggg gttcttcatg tcgtagtacg
     1921 tcgtcaggtc attgaaggtc cgcaccgcgg gcaggctctt gtacgtggcg gccttactgt
     1981 tgtagtgcag cgtgatggcg tcggtccggg ccagcgagaa ggtcgagtcg aaggtctgga
     2041 cgcggttgcg ccacaccgcg ccgtctgggc gcttgagcgc ctggggatgc gcgtcgacgt
     2101 agagcagcat gccggtgccc gggcggctcg ggtcggtgtc gttgttggcg taccgcgtgt
     2161 tccagtagct caccagcatg ccgtcctgat acgggaagcg ctccacgtag ttctgcaggt
     2221 tgggattgtt gaggaagccg aagttgtagg ggccgacctt gagcgtgtag tcgtagcccc
     2281 agtacacctt gttctcggcg atgtagtagt gggtgtacgt cttctcctcg gtcccggtcg
     2341 tcgcgcggaa gccgtcgtag gtccagcccg cgtcggtctc ggcgccgtcc agcgcctggc
     2401 cggtgatctg gatgtcatcg accatgaacc cgtcgtagtt gacgccgccg tcgctcctgt
     2461 actggaagcc gagcatgacg tcgccggtga aggtggagag gtcggcggtc aggtcgaccc
     2521 agccgccgga gaagccctcg atgccgttgg cctcgacgct cgagttcgac aggttcgtcg
     2581 gcacggtccg ccaggtggcg ccgccatccg tggacgcgat cacgttggcg aagtcgtagc
     2641 cttcttcgat gccgtagttg accttggcgg cgagcgttga cccggcgccg agcgtgaagg
     2701 ccttggtcat cttgttgttg aggttgttgg cggacccgct gaagtagaac atcgtgcctg
     2761 cgtacggtgc gccgatgacc tcagtcacct gatactcggg aagcacgacg aacagcgcct
     2821 gcttctcgga gctgttgtac tcgctgggac cgaggacggt ctgggcggtg tcgccggcga
     2881 tggcgacgtc gtagtccagc cagccgagca ggaacttctc ccacgcgccg agatgacccg
     2941 gcttcgagcc gatgtcgtag gcgccgtcgc tcagccacga gccggaggac atgagcgtcc
     3001 agaagccggt gctgttctcg aggtagttgt agtcgtacag gtccgggacg cccagatcgt
     3061 gcgagaactc gtgcgtgaag acgcccacgc cgccgttctc gggctggatg gtgtagtcgc
     3121 cgatccagta gttggtgtca ccgatcttca ggccgccgag caggtagtcc ggcgacgggc
     3181 cggtcttgtc gatgtcggcg tagttggcgt accagctgtg gctccagatc gcgtcggcgc
     3241 cgagcgcgcc gccgccggct tcttcacctt cgccggcgtg cagcgcctgg aagtggtcga
     3301 tgtagccgtc cggctccttg aactcgccgt caccgtcgta gtcgtaccgg tcgaggaggt
     3361 cgaactgcgc caggtacgcg tcgatctcgg caggcgtccg accggccgcg atctgatcct
     3421 cgtaccagcc gtcggccatg tcctgaagca acagccagac gttcgtcgag gtgtcctctc
     3481 cgccgacgct gtcgtcgtag tagaggtaac cttcggggac cgtgacccag tcggtcgcgt
     3541 caccgtacac gccatagcgg ccgctggact gctcgaggaa gtagttgcgc atggagttcg
     3601 cgccggacga gtcgtcgtac agcagttcgt tgaagtagtc cctgctgaaa tcctgcaccc
     3661 agatcgtgct gttgtcgtag gcgcggttcg gctcggcgat gttgttgtgc gggaagtccg
     3721 cgaactcgcc gagcatcgtc cagacggggt cttcgccttc gagttcgagc ttgacgaact
     3781 ggccctcggc gacctcgacc acgccgttct tgtctttgcc cgtgagcttg ccctggagct
     3841 tcttctcaag ggcgacctgc ttcagcgccg cctgcttgtc cgccagcggg ctgtgacgat
     3901 cgtctctcct gacctgcctg ttccgcgcgt cggaggcgcc gttcgagttg gctacggcgg
     3961 cggcccacac caccggcgcg gccagtaaca gcatcaggat cgtgagcagg gtgaatctga
     4021 acagcttcga agacatctgg accccctttg ctcctcttgc agtcgccaga ctgcggtact
     4081 gactagaacg catacacccg gggaggtgct cgtgcaaggt ggggccgggc ggtccgcctg
     4141 cgcggcccgg cctgcctgta gccagggctg gctacgcggg gctatgcgcc cggaccgccg
     4201 gggccgctga ccacgctgcc ggcggtctcg ccgccgtcca tgacgagcac cgagccggtg
     4261 aagtaggcgg cctcgtcgcc ggcgaggtag gcgaagaggc cggcgagttc ctcgggcgtc
     4321 gcgtgacgcc cgagcgggat gcgccggttc acctgttcca gcatctccgg cgtgtactcg
     4381 gcctcctgca tgggcgtgag cacgtagccc gggcacaccg cgttcacgcg cacggtgggc
     4441 gcccactcga gcgccagcga cttcgtcagc gcgaccagcc cggccttggt cgcgttgtag
     4501 tcggcgtagt aacggtagcc caccagcgcg ttggtcgagg ccgtgaacag gatcgcgccg
     4561 ccgccgcccg ccagcatgct ccgcgccgcc gcctgcgccg cccagaacgc gccggagagg
     4621 ttggtggcga cgacctggtc ccactggccg gcgtcgatgt cgagggccgg gctcctgatg
     4681 ctcacgccgg cgttggcgat gagcacgtcc agcgagccga tctgcgcgaa cgcctgctcc
     4741 acctgtcgct tgtcgccgac gtcgcagggg atgacgtcgg ggccgggcgt gcgcgcgagg
     4801 atcaccacct cgcagccctc ttcccggaac cgcgccgccg tcgccgcgcc gatgccgctg
     4861 gtgccgccgg tgatgaccac cttcttgccg gccagaccct gcatgccgtc ctcctcgtcc
     4921 gccgtgggcc gctgcctgta tcctacgcgt atgacccggc cacccaagcc cttcatcgga
     4981 gtcctcagcg acacccacgg ctactacgac gcggcgctgg acgacctgct cgccggcgcc
     5041 gagcacatcg tgcacgccgg cgacatcggc acgctggagg tgctctcccg gctccgcgcg
     5101 atcgcgccgc tcacggcggt gctgggcaac accgacctcc cgttctggga gggcgacctg
     5161 ccctgggagg cggaagtggt cgtgctgggc aggcgcatcc tggtcggcca catcggcaag
     5221 agcctcatgg gccggcacga tccggtcgcc gagggcttcg acctggtcgt cagcggccac
     5281 agccacaagg ccgcggtcgt gtggcgcgag agcacgctgt tcctcaaccc cggcgccgcg
     5341 ggcaaagcgc ggttcggcca gccgcgcacg ctggccctcg tcaccatcga cgccgacggc
     5401 cagctggcgc cggagatcgt gccgctggac tgagcgcgcg gaggcgccgg ccggccgggc
     5461 cggccccgag cgccgccggg ccgatcagcg ggcgcgcacg gcctcgtagg cggcgagcgc
     5521 gcgggagcgg gcctcgccgt gatccagcag gggctccgga taggtctcgc cgaggctgac
     5581 gcccgcggcc ggcagggcgc cctccggcgc cgtccacggc gcgtggatgt cccgcgcgtc
     5641 caggccgccg agttccggcg cccagcggcg gacgtaggcg ccgtcggggt cgtagcgcct
     5701 gccctgcgtc acgggattga agatgcggaa gtacggggcc gcgtcggcgc cgcacccggc
     5761 gacccactgc cagccgagcg tgttgtcggc gaggtcggcg tccaccagcg tgtcccagaa
     5821 ccacgcggcg ccgctctgcc acggcagcag gaggtccttg acgaggaacg agccggccag
     5881 cagacgcgcc cggttgtgca gccagcccgt ggtccagagc tcgcgcatcc ccgcgtcgac
     5941 gatcgggtag cccgtccggc cgcgccgcca ggcctcgagc gcggccgggt cctctcgcca
     6001 ggggaacgcc gcgaaacggg cgtgcagcgg ctgcgtcgtc gtgtgcggga acgcggccag
     6061 caggtggtgg ccgaactcgc gccagccgag ctggcgcagg aaggcgccgg cggagcgccg
     6121 cagacccggc gcctgctccg cgtcccagtt cgtggggccg agcgcggcct cgacctcgag
     6181 cccggcctcg gcgagcgtcc cggagacggc gtgccacacc tggcgcgccg tcacctctcc
     6241 ccagcgcagg tggggcgaga gccgcgaggt gccctccgtc ccgggtgcgt ccctgccgga
     6301 ggcgtagccg gcgagcgcgc cctcgaggaa ggcctccagg cgcgcgtgcg cgcccgcctc
     6361 gccggggcgc cagacgtcgc cgaacccagc cgcccagggc cgcagcgcct ccgcctccag
     6421 cgccgagagg gcgaggcccc gcgggtccgc gggtgcgggg ggaagggccc ggggagccgg
     6481 cagaggttcc tcgggcgcgc cggccgcgag gcgggcgcgc cagaacggcg tgaagacggt
     6541 gtacgggcgg ccgtcgcgcg tgcgcacgga gagcgggtct gcgagcaggt ttgcctgggg
     6601 cgcgacgacc gcgccgatcc cgcgggcggt gagcgccgcc tccagggtgt cgtcgtcggc
     6661 cgtctcgtcc ggctcgaggc cgcgtgccca caccacggcc tcggcggcgg ctcgctccgc
     6721 cagcgcggcc accgcggcga cagggtcgcc gcgctccacc agcagtcgcg agcccagacg
     6781 ccccaggccg cggtccaggc tcgcgagcga atgccagagc caccagcgcg ccgcgccgcc
     6841 gggggcccag gcgcggcgct cccccggttc ccacacgtag accggcagga gtgagccgcc
     6901 gccatcctcc gcgatccgtg cggcggccag caacgccggg ttgtcgccga gccgcaggtc
     6961 gcggcgcagc cacaggatcg tcggccgtga tctgccgggg tcggtctcgg tcgtctgggc
     7021 tcccctgagt cggaggactg cggcggcgct tcgtgatgga atatgaccac aggccgccgc
     7081 gcgcaatcgc cgcggcgggc gctcgcgggc cgaagggagg gagacgtgag cggcgggacc
     7141 acgccggagc accaggccgc gctgcggcgc ctgcgggaag gctggggcag gatggacggg
     7201 cgcgtcgtgc tggtcaccgg cgcgacgcgc ggcatcggcc tggagacggc cgtccggctg
     7261 gcggggctgg gtgcggacgt gctcgtgcac ggccgcgacc cgggccgcgg agcggccgcc
     7321 ctcgcggccg tgcgcgccgc ctccacacct gcggccgcgc cggcgctgta cctcgccgac
     7381 ctcggcgctc cggcggacgt ccgccggctg gcggccgagg tgacgcgcga ccgcccgcgt
     7441 ctggacgtcc tcctcgccaa cgcgggggtc tacgcgcccg agcggcgcgt gtctgccggc
     7501 ggcctcgagc tcaccctggc cgtcaacgtc gtcgcaccga tgctcctcgc gtgctcgctc
     7561 ctcccggtgc tgcgcgccgc tgcgcccgcg cgcgtcgtgg tcctgagctc ggcgtcccac
     7621 tggaccggcg agatccactg ggacgatctg cagctcgccg cgcccggcgc ctacgacggc
     7681 ctgcgcgcct acgaccagtc caagctcgcc gtgttgatgc tgaccctcgc cctcgcacgg
     7741 cggctgcgcg gcagcggagt caccgccgtg agcctcgatc ccggggacgt ggccaccacg
     7801 ctgctggcga gcgggtggcc cggcctcccg ggcatctccg tcgagaacgg agccgtgacc
     7861 agcgtgtacc tggcgtcggc gccggacgcc gccggcgtct ccggcgcgta cttcgaggac
     7921 ggcgccgccg tcatgccgct ggcggcggcg ctcgacgtcg cggcgcaggg ccgcctgtgg
     7981 ggcctcgtgg aggatctcgc aggacccttc agcgtgtagc gccgggtata tctgcagcgg
     8041 aacggttcgg tacgaccgcg cggctgggcc gcgcgtggag gatcgcgatg accaggacac
     8101 caccggtgac gatctactcc cagacgacct gcggctggag ccgcaaggcc aaggagtact
     8161 tcaagcagcg aggcatcaag gcgttcgtca tcgaatacga caccgcgggc cccgccctgc
     8221 agcagaagat ctccgccgag atgcgcaagg agggggcgga cgggttcccc ttcgtacgca
     8281 tcggcggccg ggtggtcaag ggctacgacc cggccaccta cgacaggctc ctgcgcgggg
     8341 cctgagagac tcgagaatgc cgggacagag gagacgcgag gtggccgagc agaccaaggt
     8401 cgagttgtac acgctgagca cctgcccgtg gagccgcaac acacgcgcgt ggctcgacaa
     8461 gcgccacgtg gcctacgact tcgtcgacta cgacctcgcc gaccagggcc tgcaggcgca
     8521 gatccaggag gagatggtgc gccgcggcgc gtcggcgttc ccgttcgtca ggttcggcga
     8581 gacggacttc atcatcggtt acaaccccga cgcgttcgcg cggctgctcg ggttgcccac
     8641 ggacgcggag ctcgatgcgg cgaagggcga agaaggaccg gcgaagggtg ggggcgcggg
     8701 ctgaagctgg tcggggtggg cggatttgaa ccgccgacct ctcggtcccg aaccgagcgc
     8761 tctaccaagc tgagccacac cccgacgcgg ggacggattc taccggaagc gggccgggga
     8821 agctaccgct cgccggcccc tgttcgtcgc tcctccaagt gaacatttcc tttcattgac
     8881 agtcaatgtc gctcgtcagt agggtcggga gcggtagggc cgtcctgagc caagggggct
     8941 cgtgggtgtg gcggcggagg ttctgctcaa gggggagtca tgagtcttgc tcgcaagggc
     9001 gtgctgctcg cgatcgtcgt ccttctcgtc ttactcgccg ccgccggggc cgcgctcgcg
     9061 gccaagccga cgaaggtcgt catcatcacc atggaccaga tgaagccgtg gtatgcgagc
     9121 gccttcgaca tggacaacat cctctggctc cagagtcgcg gcgccaactt caagaacgcg
     9181 accgtcggcc agatggcgtc cgagacggtc gtgagccaca acaccatcgt cagcggccag
     9241 ttccccaagc acatgggctg gaccgacgag gtcatgcgcg acacctccga cgtgctgggc
     9301 tacggcgccg gcgccatcgt caccgtgggt gacctcacct acgagcagtt cacgctgctg
     9361 gtggaggccg agggctatcc caagctcggt gactacatgc acgccaagtt cccgggcaag
     9421 gtcgtcgcca acttcggcgg caagtactac caggtcgcct ccacggccgc ctcgagctca
     9481 gacatctggg tgaccttcgg aagcaaggca cagacgtcgg ctcttcccgt gccggaggcc
     9541 ctcccgtggt ccggcaccta ccgcggcccg tccggaggca acgtgcccgc gtacatcgcg
     9601 gacgacgacc gcttcaagat cagcaccggc aacgatttct cgaccgccag cccgtctccg
     9661 gcgccgaacg actactacgg caccaagacc gacaagccgg cctacctcta tcccgaggac
     9721 gggcgcatgg tcccgggtcc gtacgagacc aacctcggcg gcgacgactg ggtggccgac
     9781 gcggccgtca aagtcatcga gaacgaggac tggtcggcgc tgcacctcaa cttcagcggc
     9841 atcgacaaga tcggccacat gtggggcggc ggcaacgtcg acaaggtctc gaggtacgac
     9901 tgggatcccg ccacgatcat ggcccaggtg cacatgccct ggatcgccaa gaacgccgac
     9961 gaccaggtcg gcaaggtcat gcgggcgctc aaggctgaag gtgactggag cagcacgctg
    10021 ttcatcgtgc tcgccgacca cggctcgacg tgggcgaaga gagcccacta cgtcgatgcg
    10081 gcgggtggtg gcaacctcag ctggtactac gacccgaacg aggtcgccaa gaacaccacc
    10141 tacggccgtt tcggcgccaa caacgaggct gtcctggcgc cgctgaacgc gacaggcaac
    10201 ctggcctaca gctaccagtc gaccgccatc gaggcatggc tcatcgaccg cagctggacg
    10261 aagaaggccg aggccgccgc ggcagtgaag gccatgccgg acgtgatcgc cacgtacgtg
    10321 cgctggggcg accgctacgt gctcacctcc aggggctcca tgaccagggc cgagcggatc
    10381 tggtgggagg gccacggcca gcggctcgtc aacaccatgg cttcgcccac cggtcccgac
    10441 gtcgtcggtc tcctcaagga ccagaccggc tatggcgtgt acggcgacca cggcggggcg
    10501 cagagggaag tgcagcgcgt cccgatggtg atgtacgtga agggcatgaa gcccaaggtc
    10561 agcacggcgc cgttccggct cgtggacgtc cttccgacgg tgctctacaa catgggcatc
    10621 aagaagatgg cgccgatgga cggccgggcg tacagcctgg ggctctgaac ccgatcgtca
    10681 gcctcgactg agaagcgagg acgggcgggg cggccgcgag gccgccccgc ccgttttgcg
    10741 tgtggccggc tgtcggctgc gacaggcggg gaccgctcat gtccgttgcc agtcctggcg
    10801 acaggacccg ggggccattg acagcggccg cggattccat agcctgcttg tatcgaaccc
    10861 ttccgggcgg gggcccggtg gggcacgcac cgtgaagggg gatctatggg taccgcacgc
    10921 aagagtctgt tgatcgcgct ggtcgtgctg ctgatcttgc tcgtcgccgc gagcacggcc
    10981 ttcgccaagg cgccgacgcg ggtcatgatc gtcgtcatgg accagatgag gcctgagtac
    11041 gcgcagcagt acgacatgac caacgtgctg tggctgcaga accgcggcgt caacttcaag
    11101 aacgcctacg tcggcgacat ggcctccgag acggtggtca gccacaacgt catggtcagc
    11161 ggcctgttcc cgaagcacat gggctggtcg gatgaggcga tccgcgacgt cgacaacgtc
    11221 ctcggctacg gcgccggaaa gatcgtcacc gtcggagacc tgggctacgc cgactacgtc
    11281 aagctgatca cggcggcggg agactacccg aagctcggcg actacctgca cgccaggttc
    11341 cccggcacca aggtctgcaa cgtggggcag aagggctacc aggtcgagtc catggcggcc
    11401 ctgagctccg actactgggt ccgcatgggg agcaagaaga acaccgccga tctgacaggt
    11461 gtcttcagcc ccgccagcgt gccctgggtc ggcacctacc gcggcgtggg cggcaacgcg
    11521 cccgactaca tcaagaacga cccgcgctac ctcgtcagcg tgggcaacaa ccgtccggac
    11581 acggatcccg cgtggccggc cgactactac gggacaagga ccgacaggcc tgcctggctg
    11641 tacccggagg acggccgcta cgtccccagt ccgtacaccg acccggccgc ctatcacgag
    11701 agcggtgacg cgtgggtggc cgacgccgcc atcgagatga tggagaacga ggactggtcc
    11761 gggatgttcg tcaccctcag cgcgatggac aagatcggcc acatgtgggg tgcgggatcg
    11821 gtcgacgacc tgcagcacta cacgtgggac ccgagctcga tgttcgactg ggtccacatg
    11881 ccgttcgtgg ccaagaacgc cgacgaccag ctcggccgcc tgatcgacac gctgaaggcg
    11941 aacgatcagt tcgacgagac gctcatcgtg gtcacagccg accacggtgg ccaggactcg
    12001 atcaacgggt actacggcca cgacaagtac gacggcggca attcgagctg gtacgcgggg
    12061 acctggttgc cgggctacgg ctcggacccg acggtgccca acaccaccgc cgggcccgac
    12121 gccctgcagc cgctcatggc taccggcaac gtcgatttca gctaccagtc cacggcgatc
    12181 gagagctggc tcaaggacgc ctccgcggcc aagaaggccc aggcggccaa catcatgggc
    12241 acgctgccgg gagtggtcgc cacctacaag aagagcgccg acagcacgcg ctacgttctg
    12301 cactcggcca ggacgagcac gcgcatgaac ccgtccgagc tcgcctggtg gcagctgcac
    12361 ggccaggagc tggtcgacac gatggccttc gagggctcgg cggacgtggt aggcctgctc
    12421 aaggaccgcg tcggctacgg cgtgttcggc gaccacggtg gtgcgcagaa gcaggtgcag
    12481 cgcatcccga tgatcgtgta caacccgagc atcccgcgcg ccgtcagcgg caacggcatc
    12541 cgcctcgccg acctgatgcc gacggtgctc aggacgatgg gcatcccgct cacggcgccg
    12601 gtcgacggcc gcgcctacaa gctgccggtg ctgccctgat cgtatcgtga acacagtcgg
    12661 tgtcgatcga cgctgacgat caagaagggg cgggggcgcg caaggcgccc ccgccccttc
    12721 atcgcgtgcg gagccgggcg gctgacgtgc ctttcgatct gccgaccccc cggggttgcc
    12781 gaccgggtcc tctgttcccg tgtctacaac gtatcgacat cttggacgtt gcagcgggat
    12841 cggccccgga gcgtatctac atcgtagaga cgttcagaga ggaccctgaa ggggcgccgt
    12901 ggggtgccgg gcggcggccc gcgtcgcgtc tcagccgccg cggccaccgg cgccggcgcc
    12961 ggcggtgcgg ccgagcgacg agagcagctt ctggatgctc tcgttgatgg cctcggccgt
    13021 ctcgtgcacg gccgccttct ccgggtagat ctcgtagcgc tcgcgctctt cgcgtggcgt
    13081 caggttgggc atcaccacgt tggcgccgcg ctgcagcccg tgggcgcggc cgccggcctt
    13141 gttgaccagc gagagggccg tggtggccgg caggttggcg tccggccgcg ccaggcgggt
    13201 cagggcgacg accttgcagg tcatgagctc cgtgttgggc gcctggtcgg gcggccagtc
    13261 gtcttccgcc aggcgccgct cgaactcccg gcccagcggc gtggcggggt gggggaggta
    13321 ggggccgatg ccgatcatgt ccatgtccat tccgcggaac agctcgatgt cgccggcgac
    13381 gctggcgtgc gtctggccgg ggatgccgac catgatgccg gtgccggcct cgtagccgag
    13441 ctcctgcagg cggcgcagga tgcgcatgcg gtcgctgacc ttgcccggca ggtcgggatg
    13501 gatgtgccgg tagagctgct cgtcagaggt ctcgaagcgc aggaggtagc ggtcggcgcc
    13561 ggcctcgcgc cagagggcga gatcctcgtc cggccgctcg ccgaggctca gcgtgatcgc
    13621 ccagacggtc gtctccgcgc ggatgcggcg cagcacgccg gccatccact cggcggtgat
    13681 gccgtagtcc tcacccgcct gcatcaccag ggtgccatag ccgaacgcga ccgccttgcg
    13741 ggcgcagtcc acgatcacgt cttcgctcac gcggtagcgc tggatgccgc gattccctgc
    13801 gcggatgccg cagtaggtgc agccgcgcac gcagtagttg ctcacctcga cgaggccgcg
    13861 gaggtggacg gcgtcgccga cgtagcggcg ccgcgcctcg tccgccgcgg cccacagcgt
    13921 ctccagccgc gcctcgtcgg tctcgcgcag ccaccactcg atggcgtcgc gcgtgagcgc
    13981 gtcggcgacg ccgagcggcg gggcggcggg gcggctcacg tcagcagaga tcgtcgacgg
    14041 cggcgccgtc cggcaggccg gcggtcaccg gctccgccgg ggcgtgcccg gcccgccgcg
    14101 cctgcgccgc ccgcagcgcc tcgagggcgg ccgggaacgg ctgcagggcg cgctcgagga
    14161 tcccgaggct gaaggcgatc acaaggccgt agttggtgat cggcacgccc tgctggcggc
    14221 agcgcaggat gcgccccagc atcgcccggc ggttgaaggt gcaggcgccg cagtggatca
    14281 cgaggtccca cctgctgagg tcgtcgggga agtcgtgccc ctgcatgtgc tcgatgtcga
    14341 gcctgccgcc cacgtactgc gtcagccagc gcgggatctt gacgcgaccg atgtcctcgg
    14401 cgatggggtg gtggctgcag gcctcggcga tgaggatcct ggcgccgctc ttgaggtcgt
    14461 ctacggccag ggtgccctcc acctggctca cgagatctcc cttgaagcgg ctgaagagga
    14521 tgctgaagct cgtcatcggc acctcgggcg gcgtgtcggc cgccaccttg aggaacgcct
    14581 gcgagtcggt gaccacgagc ctgggcggcc ggttgaggcg ctcgatggcg ttgcggagct
    14641 cgcgctcctt caccacgagc gccatggcgt cgctgtccat gaggtcgcgg atgctctgca
    14701 cctgcggcag gatcaggcgc ccgcgcggcg cctccttgtc gatcggcacc acgagcacgg
    14761 cgaggtcccc gctgttgacg aggtcgccga ggatcgaggg gttgtcgatg aagtccggcg
    14821 gcgccgcgtc gagcagcgcc tggcggaagt cgagcacgcc gcggcccgcg ctcgccgccg
    14881 tgagcacggg cttgatcttc aggtcctcca ggcgcttcac cggcgccggc cggtcgatga
    14941 ggtcgctctt gttgaagacc acgacgatcg gggtcttgcg ctcgaggaac tcatccagga
    15001 tgccgtcctc gtactccccc cagctgccgg cctcggtgac cagcacgccg aggtcggtac
    15061 ggtcgaacac ctgggtggtc ttcctggcgc gcagctcgcc gagcacgccg acgtcgtcga
    15121 tcccggcggt gtcgatgaag agcacggggc cgagcggcag caactccatc ggcttctcga
    15181 ccgggtcggt ggtggtgccg gcgaagtcgc tgacgatgct gacctcctgg cgcgtgatcg
    15241 cgttcagcag gctcgacttg ccgacgttgc ggcgcccgaa gatgccgatg tgcaggcgca
    15301 tgcccttcgg tgcttccatg agactcacgc gagcacctcc ggctcagtct tccacgccgc
    15361 ggccgcgggt ctggggcgtc cccagacggc acacggcctc ggcggtcagc agttcctcgt
    15421 actgcgcggc ggtcaggacg ccgtcgccga cgacgacgtc gcggatcgac gcgccgcgct
    15481 cccgggcgcg gcgggcgacg tcgcaggcgg cgtcgtagcc gatctcgggg acgagcgcgg
    15541 tggccgcggc gctggacgcg cgcacgtgcg cggcgcagcg ctcctcgtcc gcctcgatgc
    15601 cctccacgca gagccggcgg agcacgtcgt cggcgccggc gagcaggcgc aggctctcga
    15661 gcaggcagtc ggcgacgagc ggcaggaacg cgttgagctc gaggctgccg aggccggcgg
    15721 cggtggcgat ggtggcgtcg tagccgaaca cgagcagcgc cgcctggctc acggcctcgg
    15781 ggatcaccgg gttgaccttg ccgggcatga tgctggagcc ggcctggcgc cgcggcagcc
    15841 ggatctcacc gagcccggcc tcgggacccg agctcaggag gcgcaggtca cccgagatct
    15901 tcaggagcgt ggcggcatgc gccttgagga tgcccgagac ctccacgaac acgtccgcgt
    15961 tctgcgtcgc ctccgtgagg ttctcggcgc gcgcgaaccc gatgctggtg agctcgcgca
    16021 gcacgtcgac cactcggaag atgtactgcc gcggggcggc gaagccggtg ccgatcgccg
    16081 tgccgccgag gttgaccacg cgcagccgct cctcgcactt gtagatgcgc cagcggtcgc
    16141 ggttgagggc ctcggcgtag gcgcccatct cgcggcccag cgtggtgagc acggcgtcct
    16201 ggtactgcgt gcgccccacc ttgaccacgt gcgcgaactc ctgctccttg gcctggaacg
    16261 cctcctgcag ggagacgatc tgctcctcca gccggtggag gagctcgatg gcggcgaggc
    16321 gcagagcggt ggggtaggtg tcgttggtgc tctggtggag gttgaggtcg tcggacggcg
    16381 agacgcgggc gtagtcgccg ggcgccgcgc cgaggagctg cagggcgcgg ttggcgagca
    16441 cctcgttgac gttcatgttg gtgctcgtcc cggcgccgcc ctgcaggcgg tcggcgacga
    16501 tgtgctcgtc gagcaggccg tcgccgagct cgcggcaggc gcgctcgatg gcgtcggcct
    16561 tgggctcgtc ctcgccccaa acgccgagcg cgcggttggt gcgcgcgcag gcgagcttga
    16621 cgacgccgaa cgcccgggcg agctccacgt gcaccgggcg cccggcgagc gggaagttct
    16681 cgagcgcccg ctcggtgtgc gcgccgtgca ggctgcccgc cggcacggcg acctccccga
    16741 gcaggtcgcg ctcgatgcgg acgtcgtccg tcatgcgctg aggcgtcgcg tggtcagtgg
    16801 ccgaacacca tcttctggac gtgcacgccc tcgacggcgt tgagcttctc ggccatcgcg
    16861 acggcctcgg actcgctgcc gaagaactcg atcagcacga cgccgctcgg cgagcagaag
    16921 gactcgtcga cgtcgtgaag gccgatgcgt gtcttgatgt tgcagccgaa ctcggtgaag
    16981 acggcctgga cggcgcccgc ctcctggagc ctgtcggtga cgtggatgcc gaggatgatg
    17041 tggtcgtcgg tcacgctggt tccctccgtg gtcgtggggt cgtggagcgc tccgggggag
    17101 cgctcagcag agcacgtcgc ggttgccggc gcgcacctgc gcgagcatct tctccgtgtg
    17161 ggcgcggcgg acggggtcca tctccgcgac cttctgttcg atgacctgct cgccgacggc
    17221 gcgggtctgc tggctgccgt agtcgatcag gtactccagg tacgtgctca gggcgttggg
    17281 gtcgcagtgg tgcctgatgt cgcccggctt ggcgaggtcc atgaagtcca tgccggtgcg
    17341 gcccaggcgg tagcaggcgg tgcagaagct cgggatgaag ccgagctcgg agatgtcgcg
    17401 gatgacctcg tcgagcgagc ggtggtcgcc gagctggaac tgggccgcgt cgaactcctc
    17461 gtcctcctcg tacccgccgg ggttggtgcg gctgccggcg ctgatctggc tgatgccgag
    17521 ggcgaaggtc tcctggcgca ggttggcggt ctcgcgcgtg ctcaggatca tgccggtgta
    17581 cggcacggcc agccgcagga tggcgacgat cttgcggaag tcgatgtcgc cgaccggctg
    17641 cggcgggtgg ctggccatgt cggagccgct ggccggctcg aggcgcggca cgctgatcgt
    17701 gtgcgggccg acgccgtagg cgcgctcgag ctcgtggatg tgctgcagca tggcgaggat
    17761 ctcgtaacgc cagtcggcga gcccgaagag cacgccgatg ccgacgtcgt cgatgccggc
    17821 ctccatggcg cggtgcatgc cggtgacgcg ccagtcgaag tccctcttct tgccgccgat
    17881 gtggacggcg cggtaggtct cgcggtggta cgtctcctgg aagagctgga acgtgccgat
    17941 gccggaggcc ttgagctcgc ggaactggtc cacggggagg ggggcgacgt tgacgttgac
    18001 gcggcggatc tcgccgttgc cgcgcttgac gtcgtagatg gtcccgatgc tgtcgagcac
    18061 gtactggaag ccgccgtcct tggggtacga ctcgccggcc accaggagga tgcgcttgtg
    18121 cccctggtcg acgagcagct ccacctcgtg gcggatctcc tgctgcgtga gcgcgcggcg
    18181 cttgagctcc ttgttgcgca cgcggaaggc gcagtacagg cagtcgttgt tgcagaggtt
    18241 gctgatgtac agcggggcga agagcacgag gcgctggccg tagatggcct ccttgcactc
    18301 gcgcgccgtg gtgaacagct ctgccagcag ctccggatcg ctcaccgacg agaggacggc
    18361 gacctcgtcc atgtcgagcc cgtcgagctc gctcgccttg gcgaggatct cgcgcacgcg
    18421 cacggcgtcc ttggtcgcgg acgcctgcat cgcctgcgcg atggcggcct cgtcgatgaa
    18481 cgcgcctgtc tcaaccacgg ctgtcatgag ctactccctc gggtcgacag ctgaaggctc
    18541 ggcgcagtcc gccgtagccg ggatctcgaa gctgaactcg gcccactcgc cgtgcttgga
    18601 gtcggccgtg atgcgcccgc cgtgcagctg gacgatgcgc cacgagttgt agaggccgac
    18661 gccggtcccg cggcggctct tgagcgccgg gtcgtccagt cgcgagaagc ggcggaacag
    18721 cttgtcctgc tgcgactggc tgaagcccgg cccgctgttc cacacgctga tgcgcaggcg
    18781 ctcgccgtgc ttgcggtcca cgagcacctc ggccttcacg cggatgtcgc cgccctcgtt
    18841 gccgtacttg atggcgttgt ccagcaggtt gacggtgacg atgcgcagca gcgtcgggtc
    18901 gcagcacacc ggcggcagcg ccggggaggg catgcacacc atgaggtgca tgccgtgcgc
    18961 gtcggactgc gggcgcacca ggtccacggc ctcctcgagg acctcgttca tgacgtcgac
    19021 gcgggagcgg gtggcgagct tgagctcgcc gccctcgacg cgcgcgaggt cgaggtactc
    19081 gcgcacgagg ctgagcaggt actgcccctt gcgcgccatg ctctcgacct tggcgcgctg
    19141 ctccggcgtg aggtcgccca gatagccctg ggcgaggatc tgggcgtccg tcaccatgga
    19201 cgcgacggga ctcttgagct cgtggctcac gaacccgagc atctgtgtgt aggcgtagtt
    19261 ggcctcctcc agctgctcga tgcgccaagc cttctccacc gcctggctga gccgctcggt
    19321 gatcgccatc tgcagctcga tgtgctgcgg cccgtacgcg tgcggcgcgc gggagctgcg
    19381 gaagatgaac ccgaccaccc tgccttctac gctgagcggg caggtgaggc tggagcgtac
    19441 gccctcgcgc acgagcaggc gcgtcgagcg gctgcggggg tgctcgtgcc cgtaggcctc
    19501 caggtcgccg atgatgcgcg gcagcccgcc gcggaggacc tcgagcaggg agctgcccag
    19561 cagcgattcg cggtagccct gcttgacgta cagcttctcg taggcggcgc ggttgtagta
    19621 gctggtgacg tactcgccct tctcgtcgac gaaggcgagg cccatacggt cgcacgggat
    19681 gaggacctgg gtgttgtcga acaggtagtc gacgatggcg cgcagggagg ggcgggcggc
    19741 gatcttctgg ttgacggtgc gcagcagcgt ccgcgcctcg gcgccgatgg gcttgagggc
    19801 ggcgccgtcg cggaacgggt cgacgtaggt gacggcgggg cgttgcgtct gctggggttc
    19861 cttggccatc ggcctcaagt gtacctcgac cgggcggccg ggctgcctcg gcgagaggtc
    19921 agcccggccg ccggcgaggg tccgctcagc tctgcggcat gtgcttgccg cgggcggtgt
    19981 actcggtgtg cagcagcttg tggctcttgt ggccgcacgg gccgtcggtg aggaactcgc
    20041 cgtacgtggc gagcacggcc gggttctcgt gcgacttgcg cacggcgtag gccgagtcct
    20101 ccgagtagat ggccttggcc cgcgcggcgc ggatgtcggc gctggtcggg atcggctggc
    20161 caccgccgcc caggcagccg cccgggcagg ccatgaactc gatgaagtgg cactcgctga
    20221 acttgccgcc ggccttgatg tcgtccatga ccttcttggc gttggcggtg ccgtgcgcca
    20281 cggcgacctt gagggtggcg ccgtccagcc agtcccaatc gggcacgagg tgcgcgacga
    20341 tctcgggcac cggaccgacc ttgggaacgg tgatctcggc gtacttgacg ccctcgaagc
    20401 cacgcaccgg gatgatgtcg ccgtggtcgt agatgctctc gatcttctcg ccggtgacca
    20461 tctcgatgac ggtgcgcaga gcagcctcca tcacgccgcc ggtggcgccg aagatgacgc
    20521 cggagccggt ggccgagccg aacgggtcgt cgaagtcgct cttcggcagg ctcggcacgt
    20581 ggagcccggc ctccttgaac atcttgccga gctcgcgcgt ggtgagcccg tagtcgacgt
    20641 cctggaagcc ggagtcgcgc atctccggcc ggttgcactc gaacttcttc gccgagcagg
    20701 gcatgagcgc gacgctcacg atcttctcgg ggtcgaggcc gagcttgtcg gcgtagtagg
    20761 tcttgatgag cgcgccgaac atctgctgcg ggctcttggc gctcgaggcg ttgggcaggt
    20821 actcggggta gaagtgctcg aggtacttga cccagcccgg cgagcagctg gtgaactgcg
    20881 gcagggccac gctctcgtcc ttctccacga gggccttgta caggcgcagg atgagctcgg
    20941 tgccctcctc gatgatcgtg aggtcggcgg tgaagttggt gtcgaacacc ttgtcgaagc
    21001 cggcgcggcg cagggccgtg ttcatctcga aggtgaccgg cgtgcccggc tcgaggccga
    21061 acagctcgcc catggccgag cgcggcgcgg gggccgtctg gatgacgacg tgcctgtccg
    21121 ggtcgtcgat ggcggcccac acggcgtcgg tctcgtcctt ggcgtacagg gcgccgaccg
    21181 ggcagcggtt gacgcactgg ccgcagttga tgcagacggc cgtgccgagc ggcatgtcct
    21241 cgaacgtctc gatggtgacc ttgttgctgc ggccgacggc ctcgagcacg cccacttcct
    21301 ggatgtcgat gcaggtgcgg atgcagcggc ggcagcggat gcacttgttc atgtcgcgca
    21361 tcacgctgta gctcgaggtg tcctgctcga acagcggctc ctcgggatgc ccgaagggga
    21421 acgcggtgat gccgtactcc tcggccagcg actgcagctc gcagttgccg ttgcgcacgc
    21481 agctgtagca ctcgccgcag tgctcgctga ggagcagctc gagcacgtgc ttgcgcgcca
    21541 tgcgcacctt gcggctgtgc gtgttgacct tcatcgcgtt gacgaccggg aaggtgcagg
    21601 cgggctgcag cgtacgctgg ccctcgacct ccacgacgca catgcggcag acgccggcca
    21661 cgtcgaggtc gggatggtcg cacagcgtcg ggatcttgac cccggcgttc ctggcggcct
    21721 cgaggatggt gacgccgaag gggaccttgg cgtcgacgcc gtcgatggtg acgctcacct
    21781 gggcgccgat ggcgtccggg ctggtggtgg tgatgacgcg ccggtgctgc tgcgagagcg
    21841 gcgcgcgatg gatcggcgtg gtggggttgc tcatgctcac tccctcgctc tcgtcaggcg
    21901 ctcacggcgc ggtttgcgat ctcgtcgtcg aagccctcga ggatggagag gaaggcgctc
    21961 gggctggtct gcccgaggcc gcacttcgag gcgacctgca tggtggcgcc gagcgccttg
    22021 acgctggcga ggaactcggg cgggcactcg cccgtcttga ggagcttgac cgccctgagc
    22081 atctgggcgt tgcccagacg gcagggggtg cactggccac agctctcctc gacgaagaag
    22141 tcgaggaagt tctcggccac gtcgaggatg tcgcggtcgg ggccgatgac catcaccgag
    22201 ccgccggtgg ggacgtcctc gaacgccagc gcgcgcgtga agtccttggc ggggatgcac
    22261 acgccgctgg cgccgccgat ctgcacggcc ttggcgccgg tcccgcccac ctcttcgagc
    22321 aggctggcca cggtgatgcc ccacgggaac tcgtagacgc cgggcttctc gcagtcgccg
    22381 ctgatgctga agagcttgta gccgctcgac ttgtcggtgc cgaaggtctt gaaccagtcg
    22441 cagcccttgg cgaagatcgc cgccacggag gcgagcgtct ccacgttgtt caccacggtg
    22501 gggttgttga gcaggccggc gaccaccggg aacggcgggc ggttgcgcgg ctcgccgcgg
    22561 aagccctcga ggctctcgat gagggccgtc tcctcgccgc acacgtaggc cccggcgccc
    22621 atggcgacga ggatgtcgaa gtcgaagccc tcgacgccca tgatgtcgtg cccgagcagg
    22681 ccctggccgc gccgcttctg gatgacctcg ttgaggtgcg ggcgcaggta ggtgtactcg
    22741 gcgcgcaggt agatgatgcc gagcgtcgcg ccgatggcgc ggccgccgat ggtcatgccc
    22801 tcgatcacga ggtcggcgaa ccgcgtgagg atgaccctgt ccttgaaggt gccgggctcg
    22861 ccctcgtcgg cgttgcagat gatgtacttc tggtcggcct tctccgccgc gcagaaaccc
    22921 cacttcatgc cggtggggaa gccggcgccg ccgcggccct tgaggtcgct gtcgcggacg
    22981 aagtcgacca ggtcggcgcg gctcatggcg agcgccgcct tgagcccggc ttcgggctcg
    23041 atggtctcgt aggtgagggc gttgccctgc gtctcgatcg tcgcgggcat cgctcagccc
    23101 tccttggcca cggccgcggc ccggcaggcg gcgacgatct cgcgcgccgc gtccggggtc
    23161 acccgcgtgt ggacctcgtc gttcacgagc agggcggggc cctggtcgca catgccgacg
    23221 cagcttgccc actcgagggt gaagagcccg tcgtcggtgg tctggtcgaa gccgacgccg
    23281 agcgcgtccg tgagggcgtc ggcgacggcg tccttgccgg cgaagtcgca cgagagcgtg
    23341 ccgcagagcc ggatcacgaa ctgccccgtc ggggcgccgt gcaggaaggc gtagaaggag
    23401 gcgacgctgt agacctccac agggtggatg tcgaggacgt cggcgatggt ctgcatggac
    23461 tcggagtcga cctcgtggaa gcgcctcttg acatccgcga ggatcgggat gagagagctc
    23521 ctgccggggc cgagcttctc gaccagggcc tcgacgtcgg ctctgagctg gtcttgatcg
    23581 gtcatggtca ctggcatctg gagacctctc tcactgctcg aggagcttct tgaccttggc
    23641 caccagcgtc tgcggctcga ccggcttggg ctggaactcg tcgaccggga cgatctcgcc
    23701 gtccttgtcg atgttgaggc ccatggcggc gttgacgctg gtgagcatga tgatcggcac
    23761 ctcgttgccg ttcttgcgga tctcgcgcgc catgcgcagg ccgtcgtccg gctcttccat
    23821 catgacgtcg aggatgagga ggtcgggctt gatctcctcg agcttcttca tgccgtcggc
    23881 gcggttgggc gcgccgacga cctcgtagcc ctcacgctcg aggaccaggc gtccggcatc
    23941 gacgatgtcc gggtcgtcgt caacgatgag aatcttgggc accacgcact ccttcggtcc
    24001 gttgccgttc ccccgggcgc gccggatggc gcgcccacgc ggccgccgct cgccgctcca
    24061 cgcccgccac gcaggacccc acggcaggcg cggggaagac agggacgggc agaagaggtg
    24121 acgctcggtg ctggccgctc actccacagg atccacgttc tacttggcct cacatacgcg
    24181 cagtttctaa cgcatcgcgg ccgcttgtgc aagaggtcac gagcgtcgct ggttagtatc
    24241 gcacactttc gaacacgaac gaaaccggct cacggagagc gaagaagggg cgcttcagag
    24301 ttgcggagcc gccgggacgg cggcgtcggc ggcaggctcc cgggccggcg ccgcgcccgc
    24361 cggcggcgcg tcgtgcagcg tcaccgtgaa ggtgctgccc acgccgggct cgctcgagac
    24421 cttggcctct ccgtcgtaca tgttggcgat cttgcgcacc gtgctgagtc ccaggccgct
    24481 gccgaggatc ttgaccgtct gctggttctt gatgcgcgtg aactcgttga acagcttgcc
    24541 cgcctcctcc gcgctgaggc cgatgccggt gtcgctcacg gtcacggtca cggcgccgcc
    24601 cctgcggctc agcctgacct tcacggagcc gccgtcgcgg ttgtacttga ccgcgttgga
    24661 gatgaggttg ttgaagacga tctcgacctc gcccgggtcg gcgaggagct cgatgtccgc
    24721 gccgtcctcc agctcgacgg tgatgccgcg gcgctccgcg tcgacggcga acagctccat
    24781 ggacgccttc gcgagctcac gcaggtccac gcgcttgatg acgcgctcgc gctggccgga
    24841 ctcgatgcgc gtgaggtcga gcaggtccat gatgagcttc ttcatgccgt ccacgcgcac
    24901 gatgctgcgc tccaccatct gcaggttctg gtcggcctcg gtggtgcgca ggatgttgag
    24961 gtagccctcg actgcgttga gcggcgcctt gagctcgtgc gcgagcaccg agatgaagtt
    25021 gaagcggatc tcgcgcttct cctccgccag cttgcgcgcc tgcttgctca ggatgagctg
    25081 gctcgtcgcc ttgcgcaggg cgtaccggag ctcctcgggc gtgaagggct tggcgaggaa
    25141 gtcgtaggcg ccgagcttgg tggccttgac ggccgtctcg aaggtcgcgt aggccgtgat
    25201 catgatggtg aggacctcgc ggccctgggc gctcatggcg tcgaggacgt ccatgccgcc
    25261 gacgcccggc agcttgaggt cgagcagcag gaggtcgacc tcgccgccct cgccgagcca
    25321 cgcgaggtag tcctcgccgc tggtgaagta gcggaactcg tagtcggcgt cgacgagcac
    25381 gtcggcgacg tgcaccctgt acttctggat gatgcggcgc acgccgaggc agagcgcctc
    25441 ctcgtcgtcg accatgacca ccttgagcgt ggcctgttgc gggtccatga ggctctcgcg
    25501 ggctgtcggg tcggggttca tcgcgctagc tcccggtgat ctcctgcagg ctggcgggca
    25561 cgtccgtctc cgcctcgtac cgcgggaggg agatgcagaa cgtggtgccg gtggggccgg
    25621 cggccggatc ggcgttggac tccaccgtga tctggccgcg gtgcatcttg acgatgccgt
    25681 gcgtgacggc caggccgagg cccgtcccct tgccgacctg cttcgtcgtg aagaacgggc
    25741 tgaagagctt gccgatgttc tcggcgggga tgccggtgcc ctcgtcccgc acgcagagga
    25801 ggacgttctc gggggtgccg tcgaccgtga ccacgatggt gccgccgtcg ggcatggcgt
    25861 gctgcgcgtt cgtgagcagg ttggtgagca cctggacgat ctgatcgccg tcgagctcgg
    25921 cgatggggtc ctcgaggtcg tcgaccacct cgaccgagac gccctcggcc acggggatgg
    25981 tgcgcagcac ctcggccacc aggtgggtca ggtccgtcgg ctggcgcacc acccggctct
    26041 gccgggcgaa gttgagcagg ccggagatga tcttcttgca gcggttggcc tggtcgacga
    26101 tgagcttgac gtcggccgag acgtcggggt cgtcctcgca gtcctccagc agcaggttgg
    26161 cgtgcagcag gaggatgccg agcgggttgt tgacctcgtg ggcgataccg gcggcgagct
    26221 ggcccatgct ggcgagcttc tcgctcttga ccagcgcctc cttggtcgtc tccagggaga
    26281 ggttggactg ctgcagctcg tcgacggtct tgcgcaactc ctcgacggag tagggcaggc
    26341 acatctcgac ctcggccagg ccctgccaga tggccaccgc gtggtgccgg caggtgtcgt
    26401 agccgcaggc gccgcagtcg agctcgtccg cgtcggtgtg cttgttcatg cgcgcgagga
    26461 tctcctcgac ctccgtcccg gacggcatgt cgtcgaagcg ctggtcgaag ggcgcgaagg
    26521 tgcgcgagag gtcgacgtcg gcgaactcgg cgacggcggc ctcccaggcc gcctggtcga
    26581 aggcggcctg acgggcgcgg gtctcggcgc tgacgtccga gcggcgcttg aacaccggct
    26641 cctcgcagct gaagccggcg cccatgatgc agccctcgca gcagaggatg tccagcagcc
    26701 gcgcctcggt gtcgccgtgc tcgaagtcgg tgatcgcctc caccaggttc tggcggccgt
    26761 cggcgctcac gatgtcgccg ctcagaaggt cctcgtcgag gtcggcggcc tgcagcatcc
    26821 cgcgggtgat ggggaagagg gcgccgaggc tgccgtgcgg cgggtcgaag tcgtcgccgg
    26881 cccggtcgcg ggaggtgagg ccgacgccgc gcgcggcgag cagggtgcgg agctccacgt
    26941 aggtgagcgc cgcgtggacc tcgccctcga actgttcgta acgcgcctcg cccttcttgg
    27001 cgatgcaggg cccgatgaac acgatcctga ggtcgggccc gtgcttcttg tgcagcacgc
    27061 gcgcgatcgc cagcatgggg gagacgatgg gcgcgagccg gtcgaggatg tcggggtggt
    27121 acttgcgcac gtagctgacc accgccggac aggtggtggc gatgtgacgc ccgtcgtcct
    27181 gctcgagcag gcgggcgtac tccgcggcga cgaggtcggc gccgaagctc acctcgtgga
    27241 cgctggcgaa cccgagctcg cggagggcgc cgaccaggcc gcccgtgccg cactccgtga
    27301 actcggccgg atagctcggc gccacgatcg ccgcgacggg ggcgtcgccg gcgagcagtt
    27361 cctcagccgc ctcgatgccg ctgagcacct gcttggcgtt ctggctgcag acggtgacgc
    27421 agttgccgca gccgatgcag cgcgtctgga tgacgctcgc ctggccgtcc tcgatctgga
    27481 tggccttggc gggacactcg cgcacgcagg tgtagcaccg gcggcacttc tcccggatgg
    27541 tcgtgacgag ctggaggtag gggacggtct cctcgttcat ggcaggcacc cggcaggcgt
    27601 cagccgcggg cggcgagcaa ctcgccgacc ttggccagca acgcctgcgc gtcgatgggt
    27661 ttgtcgaagt aggcgtcgac gttcatcttg gccaggtcct cggcggagtc tgggcggaag
    27721 ttcaggccga gcgcgctgct caccgacgtg ctgatgatga ccggcacgtc ggcggtgctc
    27781 gggtcgctct tgagcgcggc ggcgaaggtg aacccggcgt cgatgctggt catcatgagg
    27841 tcggtgatga ccaggtccgg cgcctccgcg tcgatgcgcg cctgggcctc ggcgggattg
    27901 gtggcggtga ccacgcggta gccggctccc tcgaggatga agcggttgat ctcgaggaag
    27961 tcgaagtcgt cgtcgacgat gaggatctgt tgcttgtgct ccgcgctcat ggctcgtcct
    28021 cagtatagtc gctgtgcctt cggggcaccg ccagctgagc tgctttgcag ctcaatagcg
    28081 gggtccgcct cgcggcggcc ccggaggcgg ccccggtcac ccggggccgc ctcgcgggcg
    28141 cgccgcccac gcgcccggcc gccgtcttgc gtgcgccgcg gttcagacgc ggtcgtgctc
    28201 gaagtagtgc gtgtgcagga ggcggtgcga gatctcgccg cagggcgccc cgagatactc
    28261 ctcgtacagg gcgcagacgg cggggttctc gtgcgacttg cgcagctcgc gtccctcgtc
    28321 ctccttgtag atcgcctcga tgcgcttcag gcgcacctcg ttggtcgtga agcgcggctg
    28381 cccgccgccg ccgatgcagc cgccggggca cgtcatgacc tcgacgaagt ggtactcggc
    28441 ttcgccgttc ttcacctggt cgatgacctt ctgcgcgttg ccgaggccgt gagccacggc
    28501 gaccttgagg tcggctccct cgaggaacgc ccagtctccc ttggccccct cgaccttgac
    28561 ggaggcgacc ttcacgccct cgagaccctc gatgggcgcg acgtgcaggt tggggaaggg
    28621 gaacggcgtc tcggtgacga tctcccacgc cgtgcgcagg gccgcctcca tgacgccgcc
    28681 ggtgttggcg aagatgtcgg cggcgccggt gctgatgccc agcggcgcgt ccatcttctc
    28741 gtcgggcagg ctgcggaagt cgatgccggc ctgcttgatg aacgcgccga gctcacgcgt
    28801 ggtgagcacg atgtcgacgt cccggacgcc gctgtcgttc atctccgggc gtgcggcctc
    28861 gtacttcttg gcggtgcagg gcatgaccga gaccacgacc atgtcctccg gcttcacgcc
    28921 gatcttctcg gcgtagtagg tcttggcgac cgccccgaac atctgctgcg ggctcttgca
    28981 ggtgctcagg ttggcgagca tgtccgggtt gaagtgctcc atgtagttga tccagcccgg
    29041 cgagcagctc gagaactgcg gcagcgccac ctcttcgccg tcgacgagcg ccttcttgag
    29101 gcgcacgagc agctcggtgc cctcttcgag gatggtgagg tcggcggtga agtcggtgtc
    29161 gaacaccgcg tcgaagccca tgcggcgcag ggcggtgacc atcttgccgg tcacgagggt
    29221 gcccggcgca tagtcgaagc actcgccgag ggcggcgcgg atcgccggcg cagtctggac
    29281 gacgaccgtc ttggtcgggt cctcgagcgc gtcccagacc tggtcgatct ggttgcgctc
    29341 ggtgatcgcc ccgacggggc agacggcggc gcactggccg cactgcacgc acaccacctc
    29401 gctgaggtcc tgggcgaagg cggggccgat cagcgtggcg aacccccgtc cctgggcgaa
    29461 cagcccgccg acgtgctgga cctcgttgca gacagtgacg cagcgc
//
LOCUS       DASUMX010000064         3862 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_305610, whole genome shotgun sequence.
ACCESSION   DASUMX010000064 DASUMX010000000
VERSION     DASUMX010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3862
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305610"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /gene="leuD"
                     /locus_tag="VFZ86_02665"
     CDS             complement(<1..254)
                     /gene="leuD"
                     /locus_tag="VFZ86_02665"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015908449.1"
                     /note="catalyzes the isomerization between
                     2-isopropylmalate and 3-isopropylmalate in leucine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEX5641222.1"
                     /translation="MIFEGTVHKYGRDVDTDVIIPARYLNTNDPDELASHAMEDIDAG
                     FVDRVQQGDIIVADENFGCGSSREHAPVAIRYSGVSCVVAS"
     gene            complement(251..1549)
                     /gene="leuC"
                     /locus_tag="VFZ86_02670"
     CDS             complement(251..1549)
                     /gene="leuC"
                     /locus_tag="VFZ86_02670"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEX5641223.1"
                     /translation="MGSTITEKILARAAGRDEVHAGDIIEAKIDLALANDITAPLAIA
                     EFEKAGFAKVWDPARIALVPDHFTPNKDIKAAGQVKKVREFARRHEIVNYFEVGRVGI
                     EHVLLPDSGMVAPGDVVIGADSHTCTYGAVAAFSSGVGSTDFAAAMAWGHVWLRVPET
                     FKFVYSGTPGPWVTGKDLMLHTIGLIGVDGALYQAMEFAGPAIEALSMDERFTMCNMA
                     IEAGGKSGIVGFDDKTTAYLQSRPDGSRARTAGQVFAGDRDADYARVLEIDASAVQPT
                     VSKPHLPSNTAAAADCGDIAIDQVVIGSCTNGRIADLRQAASILRGKQVHPDVRCIIL
                     PGTQDVCLEAIADGTMQALIEAGCAFSTPTCGACLGGHMGVLAEGERAVATTNRNFVG
                     RMGHTGAEVYLASPYVAAASALAGHIATPEEVGAVKGGSR"
     gene            complement(1549..3084)
                     /locus_tag="VFZ86_02675"
     CDS             complement(1549..3084)
                     /locus_tag="VFZ86_02675"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEX5641224.1"
                     /translation="MSSTRYIRIFDTTLRDGEQAPGATLNRAEKLEIAHQLAKLRVDI
                     IEAGFPVASPDDFAAVKAIATEVEGPRIAGLARAIEEDIRVCWDAVKGAGVPRIHTFI
                     STSPVHMQYQIKKTPDEVLADTRAMVTLAAELVHEHPEADLEFSAMDASRSDPEFLAQ
                     VLDLAVQCGATTINVPDTVGYALPLEFGDFIRELYRLAPSLHDVHVSVHCHNDLGLAT
                     ANSLAAVRAGATQVECAVNGIGERAGNASLEEVVMAIRTRGEYFQSETGVDTREIART
                     SRLVSSLTGYVVQRNKAIVGRNAFAHESGIHQAGVLSEASTFEIMTPADVGLADNDLV
                     LGKHSGRHALRAKLAELGYTLSDAELGDAFKKFKDVADKKKAVTVLDLEALVSEEIRE
                     REDHFTLTSFVNQSGSAIIPTSQVEVLARGEVKRGRSFSNGSVESVFAAIDDAVGISG
                     SLADYQVRAISSGKDSLAEVRVSVEVNGRSFNGQAVSFDVMEASAKAYVRAVNNAAAA
                     GEV"
     gene            3402..>3862
                     /locus_tag="VFZ86_02680"
     CDS             3402..>3862
                     /locus_tag="VFZ86_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641225.1"
                     /translation="MFRRAFCTVVLATVILTCAAAPAAAREARPSQAVHTGSATAVTF
                     TPRLTMVRYARAVSVSGFLTSDGDPVAGATVTLRETRGGVTRDGGTAVTNGQGLYQTT
                     VSPRFNASWHAEAAGVSSASVVIQVRPRVTLALSHTQSGARLTEIFSGAVAP"
BASE COUNT          506 a         1360 c         1360 g          636 t
ORIGIN      
        1 gacgcgacca cgcagctcac gcctgagtag cggatcgcca ccggagcgtg ctcgcgcgag
       61 ctgccgcagc cgaagttctc gtcggcgacg atgatgtcgc cctgctggac gcggtccacg
      121 aacccggcgt cgatgtcctc catcgcgtgc gaggccagct cgtccgggtc gttggtgttg
      181 aggtagcggg ccgggatgat gacgtcggtg tccacgtccc ggccgtactt gtgcacggtg
      241 ccctcgaaga tcatcgcgag cctcccttca cggcgccgac ctcttcgggg gtggcgatgt
      301 ggccggccag tgcggaggcg gcggccacgt acgggctcgc caggtagacc tcggcgccgg
      361 tgtggcccat gcggccgacg aagttgcggt tggtggtggc cacggcgcgc tcgccctcgg
      421 cgaggacgcc catgtggccg cccaggcagg cgccgcaggt gggcgtgctg aaggcgcagc
      481 cggcctcgat gagcgcctgc atggtgccgt cggcgatggc ctcgaggcag acgtcctggg
      541 tgcccggcag gatgatgcag cgcacgtccg ggtggacctg cttgccgcgc aggatgctcg
      601 ccgcctggcg caggtcggcg atgcggccgt tggtgcagct gccgatgacg acctggtcga
      661 tggcgatgtc gccgcagtcg gcggcggcgg ccgtgttgct cggcaggtgc ggcttgctca
      721 ccgtgggctg cacggcggag gcgtcgatct cgagcaccct ggcgtagtcc gcgtcgcggt
      781 cgccggcgaa cacctgaccg gccgtgcgcg cgcggctgcc gtccggacgg ctctggagat
      841 acgcggtcgt cttgtcgtcg aagccgacga tgccgctctt gccgccggcc tcgatcgcca
      901 tgttgcacat ggtgaagcgc tcgtccatgg acagcgcctc gatggccggg ccggcgaact
      961 ccatcgcctg gtacagggcg ccgtccacgc cgatgaggcc gatggtgtgc agcatgaggt
     1021 ccttgccggt gacccagggg cccggcgtgc ccgagtagac gaacttgaac gtctcgggca
     1081 cccgcagcca cacgtggccc caggccatgg cggcggcgaa gtcggtgctg ccgacgccgc
     1141 tgctgaacgc ggccacggcg ccgtaggtgc aggtgtggct gtcggcgccg atgacgacgt
     1201 cgcccggcgc caccatgccg ctgtcgggca gcagcacgtg ctcgatgccg acacgcccga
     1261 cctcgaagta gttgacgatc tcgtgcctgc gcgcgaactc gcgcaccttc ttgacctggc
     1321 ccgccgcctt gatgtccttg ttgggcgtga agtggtccgg caccagggcg atccgggccg
     1381 ggtcccagac cttggcgaac ccggccttct cgaactcggc gatcgccagc ggcgcggtga
     1441 tgtcgttggc cagcgcgagg tcgatcttcg cctcgatgat gtcgccggcg tgcacctcgt
     1501 cgcgccccgc ggcgcgggcg aggatcttct cggtgatcgt agagcccatc agacctcccc
     1561 tgcagccgcg gcgttgttga cggccctcac gtaggccttc gccgaggctt ccatcacatc
     1621 gaaactgacg gcctgcccgt tgaacgagcg gccgttgacc tccaccgaca cgcggacttc
     1681 cgcgagcgag tccttgccgg acgagatggc gcggacctgg tagtcggcca ggctgccgct
     1741 gatccccacc gcgtcgtcga tggcggcgaa cacgctctcc accgagccgt tgctgaagct
     1801 gcggcccctc ttgacctcgc cgcgcgccag cacctcgacc tggctcgtcg ggatgatcgc
     1861 cgagcccgac tggttgacga agctcgtgag cgtgaagtgg tcctcgcgct cgcggatctc
     1921 ctcgctcacc agcgcctcga ggtccagcac cgtgaccgcc ttcttcttgt ccgcgacgtc
     1981 cttgaacttc ttgaacgcgt cgccgagctc ggcgtccgac agcgtgtagc cgagctcggc
     2041 cagcttggcg cgcagcgcgt gccgcccgct gtgcttgccg agcacgaggt cgttgtcggc
     2101 gaggccgacg tcggcgggcg tcatgatctc gaaggtggac gcctcgctga gcacgccggc
     2161 ctggtggatg ccgctctcgt gggcgaacgc gttgcggccg acgatggcct tgttgcgctg
     2221 cacgacgtag ccggtcagcg agctgacgag acggctggtg cgggcgatct ccctggtgtc
     2281 cacgcccgtc tcgctctgga agtactcgcc gcgcgtgcgg atcgccatca cgacctcttc
     2341 gagggaggcg ttgccggcgc gctcgccgat gccgttgacg gcgcactcga cctgcgtggc
     2401 gccggcccgg acggcggcga gcgagttggc cgtggccagc cccaggtcgt tgtggcagtg
     2461 cacgctcacg tgcacgtcgt gcagcgaggg cgccagccgg tagagctcgc ggatgaagtc
     2521 gccgaactcg agcggcagcg cgtagccgac ggtgtcgggc acgttgatgg tggtcgcgcc
     2581 gcactgcacc gcgaggtcga gcacctgcgc caggaactcc gggtcggagc ggctggcgtc
     2641 catggcgctg aactccaggt cggcctcggg gtgctcgtgc accagctcgg ccgccagggt
     2701 caccatggcg cgcgtgtcgg ccagcacctc gtccggcgtc ttcttgatct ggtactgcat
     2761 gtgcaccggg ctggtgctga tgaacgtgtg gatgcgcggc accccggcgc ccttgaccgc
     2821 gtcccagcag acccggatgt cctcctcgat ggcccgcgcc aggcccgcga tgcgcgggcc
     2881 ctcgacctcg gtggcgatcg ccttgaccgc cgcgaagtcg tcgggtgagg cgaccgggaa
     2941 cccggcctcg atgatatcca cgcgcagctt ggcgagctgg tgcgcgatct cgagcttctc
     3001 ggccctgttc aaggtagcgc ccggcgcctg ttcgccgtcg cgcagtgtcg tgtcgaagat
     3061 cctgatgtac ctggtgctac tcacgagtca cctccacctc ccccgaggca tgcgtcgtca
     3121 tctgatgaaa cgtggatccg ggtgtgatcc gagggtggcc gtgcaggccg caggtgtcta
     3181 cgcatccccc cggaggggga gcagcagcag gcagccgagg agggagaggt cgcgggcgcg
     3241 taccccaatg tgggcctccc ggcgtgtctc gcttgtggca cggcgacgca tggtccggag
     3301 agtctacgcc acgcgtacgt gcaggcgcaa gcatcgccgc cggccctgcg cccactctgc
     3361 tagaatgagc gcgaccaatc cgcgtctgtt ggggggccac catgttccgg cgcgcattct
     3421 gcaccgtcgt cctcgcgacc gtcatcctca cgtgcgcggc ggcgccggcc gccgcccgcg
     3481 aagctcgccc gtcgcaggcc gtccacaccg gctccgccac ggcggtgacc ttcaccccgc
     3541 gcctcacgat ggtgcgctac gcgagggccg tctccgtgtc cggcttcctg acctccgacg
     3601 gcgacccggt ggcgggcgcc accgtcaccc tccgcgagac ccgcggcggc gtgacccgcg
     3661 acggcggcac cgcggtcacg aacggccagg gcctctacca gacgaccgtc tcgccccggt
     3721 tcaacgcctc gtggcacgcc gaggccgccg gcgtctccag cgcttcggtc gtcatccagg
     3781 tcaggccgag ggtgacgctg gcgctctcgc acacccagtc gggcgcccgc ctcaccgaga
     3841 tcttcagcgg cgccgtcgcg cc
//
LOCUS       DASUMX010000065        18248 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1455353, whole genome shotgun sequence.
ACCESSION   DASUMX010000065 DASUMX010000000
VERSION     DASUMX010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 18248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18248
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1455353"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="VFZ86_02685"
     CDS             <1..296
                     /locus_tag="VFZ86_02685"
                     /inference="COORDINATES: protein motif:HMM:NF021485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2202 domain-containing protein"
                     /protein_id="HEX5641226.1"
                     /translation="AIRDLLDRYEVADPAEPDVPGEFTNHDLQGLYDDLVAQGRLSLH
                     DALEVGVAIEELDIEDLGACLQRTNHADIQNVYGNLIDGSYNHLAAFLKVLAR"
     gene            457..2286
                     /locus_tag="VFZ86_02690"
     CDS             457..2286
                     /locus_tag="VFZ86_02690"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5641227.1"
                     /translation="MQTLKRKTRWIGLLCALAALGLAALVGAGVARGEDGSTPASDEK
                     VVLKIGWMGDIDNLNPLIGWTNNVYEIYAQEYLLMVGRDWETLKPDDSGIAKSWEVSD
                     DGLEWTFAMNEGMTWHDGEPITSEDVAFTYNFIIENEIAAYISFVAGIERVEAVDDLT
                     YKVICEKPVANMPTLWIPCLPEHIWGKMSAKEASQTFQNDPPCIGSGPYQVVEWKKER
                     YLRMEAYEDFYLGAPTVDELIFVVYQNGDTMVQDLKSGSLDAAYLFPPAQFDPLKATE
                     GIEAIEYSWFNWDYVGFNCYEGESKGHPVLLDKDFRAALEYAMNRDKIVELAYSGHAW
                     PGYTFMPPDNWSDPDYAWAPAEGEARDFDPAQANALLDEAGYTDSDGDGVREFKGKPI
                     KIRLWAPAESPESQRAGKLIAGWWKDVGVDVALSVQDEGVYFDNLWAYEGDTFVPDFD
                     AYLWSWDGYIDPGQSLTCYTTAQIEGWNEYAWSNAEYDRLDTLQASEMDPDLRADYIK
                     QMQAVMYEDSPGITTVFPFKLEAYRTDKWDGWSRSNNGNGAAFLAASMPLPYSELTPK
                     VAAESASSNLGLWVAIVVAVLVVVALAVWLILRSRRSGPAMEE"
     gene            complement(2249..2950)
                     /locus_tag="VFZ86_02695"
     CDS             complement(2249..2950)
                     /locus_tag="VFZ86_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017569696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEX5641228.1"
                     /translation="MQPRRTPSPEPRAPLSRERVLRAAVALADEAGIEALTMRELGQR
                     LGVEAMSLYNHVANKDDVLDGMVDLVVGEIDLPSDAVDWKEAMRRRAISAQAVFSRHP
                     WASVLIDSRESSGVERLRYFEWVIGTLRRAGFSVELAARAFSVLDSYIYGFGRQRSHM
                     AAGSGAEPEDMAEAFLRAIPADEYPYLRELIVEHAMDSGYDDGADLEFGLDLILDGLQ
                     RLLVTPPSRGRSGGS"
     gene            3070..3804
                     /locus_tag="VFZ86_02700"
     CDS             3070..3804
                     /locus_tag="VFZ86_02700"
                     /inference="COORDINATES: protein motif:HMM:NF025687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 family protein"
                     /protein_id="HEX5641229.1"
                     /translation="MKTSAERNLRQQKVGGFAALYLALAYLVAMPYFLVFVKYPSVVD
                     PAEKVALLVSHHDSMRAVYLVTYIVFGVVLAVLALALYARLKDGAPILAQAATATGLV
                     WGFVLIASGLVFNAGVAAVVDIHATDPAQAVSVWQAIEPVAQGLGGSGGELLGGLWVL
                     LVSVAALRTGGLPRTLDWLGVAIGAVGVLSVVPVLNDLAYGFGLLQIVWLAWLGIVML
                     PRATRASRAWRHAPSPDPAGSRADPA"
     gene            3877..5397
                     /locus_tag="VFZ86_02705"
     CDS             3877..5397
                     /locus_tag="VFZ86_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006140242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5641230.1"
                     /translation="MRNRGLGSWIARRARMSPQRPALLHEDSAWTYAELSADIARLAQ
                     ALSELGVRAGDRVGYLGPNHPAYLQTFFAAGLLGAIFVPLNHRLSASELVYVVQDAGC
                     LVVVYGAESSALVEEVRARTRVREWVAAGSVDGAQRLLGELLAGAPSDVVDLPVGLDD
                     LCFLIYTSGTTGQPKGVMLTHGNVTWNALNYLSTTDFRTDDVTLAIAPLFRAGGWGVT
                     LLPTLQKGGTVVLVSDFDPAGVLELIARHRVTTLFGGPELLTALPRTPEWISTDLSSI
                     RFVISGGDVVHESLIRAYQDRGLAFLQGYGLTEAGPMALMLDEQDALRKLGSAGRPPL
                     FVDVRIARGDLSTAEAGEVGEILVRGPNVMRGYWRRPEDSEAALVQGWLRTGDAGRMD
                     EEGYVYVIDRLKHAFVSHGQTVFPAVVERALTDHAAVAEAAVVVANDERAGQVGAAYV
                     VLADRAHATAAELLAHCRERLAWHQVPASIEFREALPKNAAGKVVKSRLAAGAPGG"
     gene            complement(5401..6540)
                     /locus_tag="VFZ86_02710"
     CDS             complement(5401..6540)
                     /locus_tag="VFZ86_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006910132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1611 domain-containing protein"
                     /protein_id="HEX5641231.1"
                     /translation="MRGKTAVVYSEAFFGTPDGKTANGLVRHSGKYEILSVIDSTRTG
                     LDSGAYLDDVPNGIPVCADLADAIAVAGRVPDFFILGVAPLSGLLSPLQRGVVLDAMR
                     RGMHIVNGLHEFLNDDLEFTAAAAKYGVLINDVRRPRAKKDLRMFTGAIDTVTCPRVA
                     VLGTDGAIGKRTTAVILTRALNERGIKAVMVGTGQTGLIQGARHGIALDAIPCQYCSG
                     EVEATVVEAFEAEHPDVIVIEGQGALSHPAYLSSTFILRGARPDAVILQHAPARRMLG
                     DFPDVPTPTPASETALLRAFAGTPVIGLTLNHEDMTDAEVSAAIILYELELGIPATDA
                     LTRPVERLVDMVLAAFPALEARLATGAVPLSSGGPETSSGSAVTS"
     gene            complement(6537..7277)
                     /gene="pepE"
                     /locus_tag="VFZ86_02715"
     CDS             complement(6537..7277)
                     /gene="pepE"
                     /locus_tag="VFZ86_02715"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007818578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HEX5641232.1"
                     /translation="MELLLLSNSTNYGGTMFSHAAEAFAGVSAGGVVTFVPYALADWD
                     DYADRAIAALGSFGIEAVSAHRADAPDRAILEAEVVMMGGGNTFRLLDSLYHLGVVED
                     LGARVREGATRYVGASAGTNVACPTIRTTNDMPICRPPSFAALGLVPFQINLHYIDAD
                     PASTFMGETRDERIAEFLEENDCAVLALYEGSWLRVSGESAVVTGRGRLFERQDVGTF
                     HQGLDVSGLLKHTPRFDGGRRPEPGGSS"
     gene            complement(7415..7615)
                     /locus_tag="VFZ86_02720"
     CDS             complement(7415..7615)
                     /locus_tag="VFZ86_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012370557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX5641233.1"
                     /translation="MAEGKVKWFNADKGYGFIEREGGDDLFVHISEIQGDVKNLTEGQ
                     AVSFTEAVGQKGKAEATQVTAL"
     gene            complement(7799..8686)
                     /locus_tag="VFZ86_02725"
     CDS             complement(7799..8686)
                     /locus_tag="VFZ86_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HEX5641234.1"
                     /translation="MAPFDFDQALDFIVTFPPMHGEQVVSEKSLTRAAWASQTPVAFK
                     LSATGTVEEPGLRYELHTDEPLSEDDVAALARRIEVYLSVYDDLSRFYALIDSDPPFV
                     AVKRELYGYHQVKFLTPFESACWTILSQRNLLGVAQHMKRTLIQEFSAGVELDGREFW
                     PFPGARSVVQASAEDLTRLLRNRRRAEYIHGAAVAFSQVDEAFFREGSYDEVSRWLRA
                     IPGVGAWSAALIMIRSLGRMERIPYGDRSLMQIAGHVYGGGEPLPADDLQRRADHYGD
                     LQGYWAHYLRVMGRVVLAP"
     gene            complement(8831..9226)
                     /locus_tag="VFZ86_02730"
     CDS             complement(8831..9226)
                     /locus_tag="VFZ86_02730"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5641235.1"
                     /translation="MKRVYVIDDEPSIVETVSLVLESNGYVVGSQNDDKDVVDNVVEF
                     GADLVILDVMLPEDTAAGFKMARALQADERTKALPRLMLTAVNESGIYVGKFSNKDLD
                     DAWLPVQEFVEKPVVPEVLLAKVRALCPS"
     gene            9298..10341
                     /locus_tag="VFZ86_02735"
     CDS             9298..10341
                     /locus_tag="VFZ86_02735"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX5641236.1"
                     /translation="MAGQEKIVVAVMGAGAQGVATLKALLAAPDVEVRYVVDPDLRAP
                     GVALAREHGIRCETHAGVVTADAAVDLILETGGDRVRADLEPGEHPDGRLMSPAGTRL
                     VASLVAEIAAVEEDARVETARYLRQASHQVKSPLTAIQSSVNVILGGYTGEIPERTRE
                     TVQKIHARIDAALAALAKRRLLADLRCVDRDALGSGTASVTEALQEAVARHAETAAAR
                     GVELRVPTGIEPDRVRCVPEHLVALFSELVENAVVYSRDGGVVEVSADVRPDGRLAVA
                     VRDHGIGVPERCLPKIFDEDYRADVAVKHYADGAGLGLAVAREIAGLHGFDLEVRSDE
                     GSGSVFTAIMSLA"
     gene            complement(10365..10820)
                     /locus_tag="VFZ86_02740"
     CDS             complement(10365..10820)
                     /locus_tag="VFZ86_02740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HEX5641237.1"
                     /translation="MRYLVGVGSYMAGDDAVGPRVVEHVTVNGLNRGFEAVDLSTDAL
                     SLVAYLSAETEAVLVVDAARLGLAPGDYLFFSPDEVETRKELSGLTTHEGDVLKVLEM
                     ARGAGYPIPPVAIMGIEPLEMESSIDLSGCLAERVPEYAAAAIERLAGL"
     gene            complement(10825..12141)
                     /locus_tag="VFZ86_02745"
     CDS             complement(10825..12141)
                     /locus_tag="VFZ86_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HEX5641238.1"
                     /translation="MSTTARIRVDHLTRVEGHGNIVVNVTDGVVEKCEWQVPEAPRFF
                     ESMVRGRHYSEVARITSRICGICSVGHTLASVKATEDALGIEVSSQSWKLRELLKHAE
                     NFDSHVLHINILVAPDLLGAPSVFPLVETHADVVARALRLKRLAHEWGSLIGGRTTHP
                     MSVVPGGMAAVPTVKELKALREQLLAAVPDLEAAVATVAALAPVVPVFDRPTEYIALS
                     NDQDYGLYDGDIQSLLPDGTTQRYPVAEYKQVTNEFVVPQSTAKYTKNKLDSYAAGAL
                     ARFNNNYDRLHPEAKKVAESLGMQPICTNPYMNTVAQAVEIVSDVYESLALLDELIAT
                     GVELEPLVEPTRYGSGAGATEVPRGILFHEYEYDDAGTCVSGNCIIPTNQNHNNIQYD
                     FEKLVPELVAQDKSEKEMELALEMLVRSYDPCISCSTHYVDVKFVR"
     gene            complement(12138..12890)
                     /locus_tag="VFZ86_02750"
     CDS             complement(12138..12890)
                     /locus_tag="VFZ86_02750"
                     /inference="COORDINATES: protein motif:HMM:NF013242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone oxidoreductase"
                     /protein_id="HEX5641239.1"
                     /translation="MGKPKVAFFDFTCCEGCQIEFTNYGDAAFLELIKHVDVVEFREA
                     MSEKTTEPIDIALVEGSFSREADRARLESIRERAAVVIAYGQCAASGGINALKNHQDD
                     YKQYVYGDDAGQPHLDSQDARPISAAIKVDYSAYGCPVNKYEVLQIVFHLLHGKAPVI
                     PNYPVCVECKRRESVCRYAEGDHCMGMVARAGCGAPCPAYGIPCEACRGFVDHPNVPA
                     LERVLVQRAGFSQKRAVEKALMFTANDLEATS"
     gene            complement(12890..13732)
                     /locus_tag="VFZ86_02755"
     CDS             complement(12890..13732)
                     /locus_tag="VFZ86_02755"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HEX5641240.1"
                     /translation="MDHQCHWTEVENIFLPREATVVRSVQVTPTETHFTLQMADDQPM
                     EYEPGQIVELGLPGYGEIPLGFASSPTRKHTFDLVVRSVGRVSGAVCGLEKGDPVFVR
                     GPLGNGFDLESLRGQDVLIVAGGIGLCPTRSLIQYILDRRGEFKRFILFYGAREPKLQ
                     LFTDDLEKWRTTHDVEFHETVDKGDEGWAGNVGVITTLFSKVELKPETRAVICGPPIF
                     YRFVIRELDQIGVSRENVFVDLERRMKCGVGKCGHCQINDKYVCVDGPVFSFADVQGL
                     EEAF"
     gene            complement(13744..14772)
                     /locus_tag="VFZ86_02760"
     CDS             complement(13744..14772)
                     /locus_tag="VFZ86_02760"
                     /inference="COORDINATES: protein motif:HMM:NF028488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX5641241.1"
                     /translation="MTVSFIRSDDFPAFVTALIEAGPVYGPVAKRSRFIFERLDTPEE
                     LRLDYDVTILPPKKLFFPPAQDLLRFDEAGFSAALEPVEKILLGVHFYDVKAIDQTDL
                     LFSERNFDVNYMAHRQTTTIVASNVQTVSSRAFFASVGADVAPQGHDAFMTAIGYGYV
                     FETLTERGEALLQHGVFQPATEAQITEAADVNAAALAQCPERLAYTTADIAAKVRAAF
                     GKDEMWATMAADCFSCGTCNTVCPTCYCFDVQDTWNVDQTSGQRTRYWDGCLTEDFAT
                     CSLGEGACENFRDERGARFRHRMMRKATYLNEKLGGPACVGCGRCSAGCTADIADPVH
                     IIDQIMAS"
     gene            complement(14925..15347)
                     /locus_tag="VFZ86_02765"
     CDS             complement(14925..15347)
                     /locus_tag="VFZ86_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide prediction"
                     /protein_id="HEX5641242.1"
                     /translation="MSRAAAYAWAAPNTALGGAAGLAVLCLGGRVRFVSGTAEFHGAA
                     VARVLGGRRGSRPYGAVTLGHVVLGASPAWLSALRGHERVHVRQYERWGIFFLPAYAL
                     SSLWQLAHGRDWYRDNRFERRPYGAGRARRRPPAGLSR"
     gene            complement(15434..15784)
                     /locus_tag="VFZ86_02770"
     CDS             complement(15434..15784)
                     /locus_tag="VFZ86_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012900229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5641243.1"
                     /translation="MPGSQEDSSEGRFTGGNALELADYQAGGVVSREIIKKKTGTVSV
                     FAFDKGEGLSEHTAPFDALVHVLDGVAEITIGGTPHTVRKDELIVMPANVPHALHAVE
                     RFKMMLVMIREPAG"
     gene            15995..16870
                     /locus_tag="VFZ86_02775"
     CDS             15995..16870
                     /locus_tag="VFZ86_02775"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX5641244.1"
                     /translation="MSDATLPSWNDRPAKAAILEFVRSVTTPGASYVPVAERIAAFDN
                     DGTLWCEKPMYVQADFVSRRWKDMVREDPRRATEQPYKALIEGDAEWFSDVYAHAPEL
                     VKGVAEAYEGITTEAFDAAAREFFASATHPTLGVPYADTAYRPMRELIGLLEANDFHV
                     YICSAGGRDFVRAVSERIYGLPQDRVIGSAATLEYRDGDLYRTAGVEQPIDDGPGKVV
                     HIWTRTGRKPLLAGGNADGDVPMLETARFALLVHHDDAEREFAYDQGAEQALAAAAAG
                     GWTVVSVKEDFRTVF"
     gene            16932..>18248
                     /locus_tag="VFZ86_02780"
     CDS             16932..>18248
                     /locus_tag="VFZ86_02780"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016883316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HEX5641245.1"
                     /translation="MGEPFKGVINVDIRDSTPDWAPFEPPKAPDDAPNVVYIVLDDVG
                     FSAMSCYGGPIETPNIDRLAAEGVRYTQWHTTALCSPTRSCLLTGRNHTRNSMACITE
                     GAIGFPNGSGTVPPENGMLSEILGELGWNTYMVGKWHLCPTDEMNLAAPRRNWPSGRG
                     FERWYGFLGAETNQWYPELVYDNHPVDQPKSPQEGYHLTEDITDKALEFIKDAHAIAP
                     DKPFFLYYAPGACHAPHHAPREWIDKFKGRFDAGYEAMRVETLARQKEMGLVPPDTEL
                     PPVNPIGTSATRKGPKGQPFPGLDLTKPWDSLSDEERRLFARMAEVYAGFLAHADHHI
                     GRLLDYLDEAELRENTLVIVVSDNGASGEGGPVGSVNENLFVNGMPDDLQTNLALLDE
                     LGGVKTYNHYPTGWAMAFNTPFKMWKRYNFNGGTSDPCVISWPAGMK"
BASE COUNT         2906 a         6197 c         6308 g         2837 t
ORIGIN      
        1 tcgccatcag ggatctcctg gaccgctacg aggtggcgga ccccgcggag cccgacgtcc
       61 cgggcgagtt caccaaccac gatctgcaag gactctacga cgacctcgtg gcccaaggcc
      121 gtctatccct ccacgatgcc ctggaggtcg gggtggcgat cgaggagctc gacatcgagg
      181 acctcggcgc ctgcttgcag cggaccaacc atgccgacat ccagaacgtc tacggcaacc
      241 tcatcgacgg cagctacaac catctggcgg ccttcctgaa ggtcctggcg cggtgaagcg
      301 acagcgcgac gcgagcacgg acggcgtcgc ctccccggct tgagtgaccg tagacgttgc
      361 ttccgcgcgc tctgtgggcc accatgaggt gaggcggccc cggcgtgcgt gctcgtggac
      421 cgcggtcaac gtgggaccgg tcagggggag acgcccatgc agactctcaa gcgaaagacc
      481 cggtggatcg gactcctgtg cgcgctcgcg gccctcggct tggccgcgct cgtcggagcc
      541 ggagtcgcgc ggggcgagga cggcagcacg cccgcgagcg acgagaaggt cgtcctcaag
      601 atcggctgga tgggcgacat cgacaacctc aacccgctca tcggctggac gaacaacgtc
      661 tacgagatct acgcccagga gtatctcctg atggtcggcc gggactggga gaccctcaag
      721 cccgacgact cgggcattgc caagagctgg gaggtcagcg acgacgggct ggagtggacg
      781 ttcgccatga acgagggcat gacctggcac gacggggagc cgatcacgtc cgaggacgtc
      841 gccttcacct acaacttcat catcgagaac gagatcgccg cctacatcag cttcgtcgcc
      901 gggatcgagc gggtggaggc cgtcgacgac ctcacgtaca aggtcatctg cgagaagccc
      961 gtcgccaaca tgcccacgct ctggatcccc tgcctccccg agcacatctg gggcaagatg
     1021 agcgccaagg aggcgagcca gaccttccag aacgacccgc cctgcatcgg cagcggcccc
     1081 taccaggtcg tcgagtggaa gaaggagcgc tacctgcgca tggaggccta cgaggacttc
     1141 tacctcggcg cgccgacggt cgacgagctc atcttcgtgg tctaccagaa cggcgacacg
     1201 atggtccagg acctgaagag cggcagcctc gacgccgcct acctcttccc gccggcgcag
     1261 ttcgacccgc tcaaggcgac cgagggcatc gaggcgatcg agtacagctg gttcaactgg
     1321 gactacgtcg ggttcaactg ctacgagggc gagtccaagg gccacccggt cctgctggac
     1381 aaggacttcc gggccgccct ggagtacgcg atgaaccgcg acaagatcgt cgagctcgcc
     1441 tacagcggcc atgcatggcc cggctacacg ttcatgccgc cggacaactg gagtgacccg
     1501 gattacgcct gggcgccggc ggagggcgag gcacgggact tcgaccccgc gcaggccaat
     1561 gccctcctcg acgaggccgg atacacggac tcggacggcg acggcgtcag ggagttcaag
     1621 ggcaagccga tcaagatccg cctgtgggcc ccggccgagt cgccggagtc gcagcgcgcc
     1681 ggcaagctca tcgccggctg gtggaaggac gtcggcgtgg acgtcgcgct cagcgtccag
     1741 gacgagggcg tctacttcga caacctctgg gcctacgagg gcgatacgtt cgtgcccgac
     1801 ttcgacgcct acctctggag ctgggacggc tacatcgacc ccggccagtc gctcacctgc
     1861 tacacgaccg cgcagatcga gggttggaac gagtacgcct ggtcgaacgc cgagtacgac
     1921 aggttggaca cgctgcaagc ctccgagatg gacccggacc tgcgcgccga ctacatcaag
     1981 cagatgcagg cggtgatgta cgaagactcc ccgggcatca ccaccgtctt cccgttcaag
     2041 ctggaggcgt accgcactga caagtgggac ggctggtcgc gctccaacaa cggcaacggg
     2101 gcggcgttcc tggccgcgtc gatgccgttg ccgtactcgg agctcacgcc caaggtagcg
     2161 gccgaaagcg cctcctccaa tctcgggctg tgggtcgcca tcgtcgtggc ggtgctcgtc
     2221 gtcgtcgcgc tggcggtgtg gctgatcctc aggagccgcc ggagcggccc cgcgatggag
     2281 gagtgaccag cagccgctgc aggccatcga gaatgaggtc gagcccgaac tcgaggtcgg
     2341 ctccgtcgtc gtagccggag tccatcgcgt gctcgacgat gagctccctg aggtacgggt
     2401 actcatcggc ggggatcgcg cgcaagaagg cctccgccat gtcctcgggc tcggctccgc
     2461 ttccggccgc catgtgggac cgctgccgtc cgaacccgta gatgtagctg tcgaggacgg
     2521 agaacgcgcg cgccgccagc tcgacggaga acccggcgcg ccggagcgtc ccgatcaccc
     2581 actcgaagta gcgcagtctc tcgacgccgg agctctcgcg cgaatcgatc aggacgcttg
     2641 cccaggggtg acgcgaaaag accgcctgcg ccgagatcgc cctccggcgc atggcctcct
     2701 tccagtcgac cgcgtctgaa ggcaggtcga tctcgcccac gacgaggtcg accatcccgt
     2761 cgaggacgtc gtccttgttg gccacgtgat tgtagagcga catcgcctcg accccgagtc
     2821 tctgcccgag ctcgcgcatc gtgagcgcct cgatgccggc ctcatccgcg agcgctacag
     2881 cggcgcgcag cacgcgctcc ctgctcagag gcgctcgcgg ctcgggactc ggtgtgcgcc
     2941 gtggttgcat ccactctccg tgcgatcgct tgacatactt acagtgtaag ttccatactt
     3001 acggcgtaag tataccgcgt ggtggtcgag gtgaacaggg tcatctgcct cggcgggaag
     3061 gagcgagaga tgaagacgtc ggcggaacgg aacctgcgac agcagaaggt ggggggcttc
     3121 gcagcgctct acctggccct ggcctacctg gtggcgatgc cctacttcct cgtgttcgtg
     3181 aagtacccga gcgtcgtgga tcccgctgag aaagtggcgc tgctggtgag ccaccacgac
     3241 agcatgcgag ccgtgtacct ggtcacctac atcgtgttcg gcgtggtcct ggccgttctg
     3301 gcgctggcgc tctacgcccg tttgaaggac ggcgctccga tcctcgccca ggcggcgacc
     3361 gcgacggggc tcgtctgggg gttcgtcctc atcgcgagcg ggctggtctt caacgccggc
     3421 gtggcggcgg tggtcgacat ccacgccacg gacccggccc aggccgtttc ggtctggcag
     3481 gcgatcgaac ccgtggctca aggcctcggc gggtccggcg gcgagctgct cggcggtctg
     3541 tgggtcctgc tcgtgagcgt ggcggccctg cgcaccggcg ggctgcccag gacgctggac
     3601 tggctcgggg tggcgatcgg cgccgtgggc gtcctctccg tcgtgcccgt cctcaacgac
     3661 ctggcctacg gcttcggcct gctgcagatc gtgtggttgg cgtggctggg gatcgtcatg
     3721 ctgccgcggg cgacgcgcgc ttcacgcgcc tggaggcacg cgccgtctcc cgaccccgcc
     3781 ggcagccggg ccgacccggc gtgaggaccg cacgagcatc acacccgacg cgcgtgacct
     3841 cggccgcgtt gggcggcaag cgggtagaac ccgaccatgc ggaaccgtgg actcgggtcg
     3901 tggatcgcca ggcgggcgag gatgagcccg cagcggccgg ccctgctcca tgaagacagc
     3961 gcgtggacgt atgcggagct ctccgccgac atcgcgcgcc tggcgcaggc gctgtcggag
     4021 ctcggcgtgc gggccggcga ccgcgtgggc tatctgggcc ccaaccaccc ggcatacctg
     4081 cagacgttct tcgccgcagg cctgctcggg gcgatcttcg tgccgctcaa ccaccgtctt
     4141 tccgcgtcgg agctcgtgta cgtcgtccag gatgcggggt gcctcgtggt ggtctacgga
     4201 gcggagtcgt ccgcgctcgt ggaggaggtc cgggcgcgga cccgcgtgcg cgagtgggtc
     4261 gccgccggga gcgtcgacgg cgctcagcgc ctcctcggcg aactgctcgc gggggcgccc
     4321 tctgatgtgg tcgatcttcc cgtgggcctc gacgatctct gcttcctcat ctacacgtcg
     4381 ggaacgaccg gtcagcccaa gggcgtcatg ctcacccacg gcaacgtcac gtggaacgcc
     4441 ctcaactacc tctcgaccac cgacttccgc accgacgacg tgacgctcgc catcgccccc
     4501 ctcttccgcg caggcggctg gggcgtgacg cttctgccca cgctgcagaa aggcgggacg
     4561 gtcgtgctgg tctcggactt cgatcccgcc ggcgtcctcg agctcatcgc ccggcaccgg
     4621 gtgaccaccc tgttcggtgg tccggaactg ctgacggccc tgccgcgcac gcccgagtgg
     4681 atctcgaccg acctctcgag catccgcttc gtcatctccg gcggggatgt cgtgcacgag
     4741 tcgctcatcc gcgcgtacca ggaccgcggc ctcgcgttcc tccagggcta cggcctcacc
     4801 gaggcggggc ccatggccct gatgctggac gagcaggacg cgctgcgaaa gctgggctcg
     4861 gccggcaggc cgccgttgtt cgtcgacgta cgcatcgccc ggggcgacct cagcaccgcc
     4921 gaggcaggag aggtgggcga gatcctggtc cgagggccga acgtgatgcg cggctactgg
     4981 cgccgccccg aggactcgga ggccgccctc gtccaggggt ggctgcgcac cggcgacgcc
     5041 ggccgcatgg acgaggaggg ctacgtctac gtgatcgacc ggctgaagca cgccttcgtc
     5101 tcgcacgggc agaccgtctt cccggcagtg gtcgagcgcg cgctgaccga ccacgcggcg
     5161 gtcgccgagg ccgccgtggt ggtggcgaac gacgaacggg ccgggcaggt gggagccgcc
     5221 tacgtcgtcc tggcggaccg cgcgcacgct accgctgcag agcttctcgc gcactgccgg
     5281 gagcgactcg cgtggcacca ggtgccggcc tcgatcgagt tccgggaggc gctgccgaag
     5341 aacgcggccg ggaaggtcgt caagagtcgt ctcgccgcag gggccccggg cggttgacgc
     5401 tcagctcgtc acggcagacc ctgatgacgt ctccggcccg cccgacgaga gcggcacggc
     5461 gccggtcgcg agtctcgcct cgagcgccgg gaacgctgcc agcaccatgt cgaccaggcg
     5521 ctcgacaggt cgcgtgagcg cgtccgtggc cgggatgccg agctcgagct cgtagaggat
     5581 gatcgccgcg ctgacctcgg cgtcggtcat gtcctcgtgg ttgagggtca gaccgatgac
     5641 cggcgtgccg gcgaacgccc gcagcagagc tgtctcgctc gccggcgtcg gcgtcggcac
     5701 gtccgggaag tcgcccagca tccggcgtgc gggcgcgtgc tgcaggatca ccgcgtcggg
     5761 ccgggcgccc ctgaggatga aggtggacga gaggtacgcg gggtgactga gcgcaccctg
     5821 cccctcgatg acgatgacgt ccggatgctc ggcctcgaac gcctccacga cggtggcctc
     5881 cacctcgccc gagcagtact gacacgggat cgcgtcgagc gcgatcccgt ggcgcgcgcc
     5941 ctggatgagc ccggtctggc cggtgccgac catgacggcc ttgatcccgc gctcgttgag
     6001 cgcgcgcgtc aggatgaccg cggtcgtgcg cttgccgatc gcgccgtcgg tgccgagcac
     6061 ggcgacgcgc gggcaggtca ccgtgtcgat agcgccggtg aacatgcgca gatccttctt
     6121 cgcccgcggg cgccggacgt cgttgatcag gacgccgtac ttcgccgcgg ccgccgtgaa
     6181 ctccaggtcg tcgttcagga actcgtgcag gccgttgacg atgtgcatgc cgcggcgcat
     6241 cgcgtcgagc acgacaccgc gctggagcgg ggagagcagg ccgctgagcg gcgccacgcc
     6301 gaggatgaag aagtcgggga cgcggcccgc gacggcgatg gcatccgcaa ggtcggcgca
     6361 gaccgggatc ccgttgggga cgtcgtcgag ataggcgccg gaatcgagtc ccgtcctggt
     6421 gctgtcgatc accgacagga tctcgtactt cccggagtgc cgcaccaggc cgttggcggt
     6481 cttgccgtcg ggggtgccga agaacgcttc ggagtacacg actgcggtct tgcctctcat
     6541 gaggaacctc ccggctccgg tcgtctgccg ccgtcgaagc gcggcgtgtg cttgaggagc
     6601 ccggagacgt cgagaccctg atggaacgtg ccgacgtcct ggcgctcgaa caggcgcccg
     6661 cggccggtga ccaccgcgga ctcgccgctg acccgcagcc aggagccctc gtacaacgcc
     6721 agcacggcgc agtcgttctc ctcgaggaac tccgcgatgc gctcgtctcg cgtctcgccc
     6781 atgaaggtcg aggccgggtc ggcatcgatg tagtgcaggt tgatctggaa gggcaccagc
     6841 ccaagcgcgg cgaagctcgg cgggcggcag atcggcatgt cgttggtggt gcgtatcgtc
     6901 gggcaggcga cgttcgtccc ggcggaggcg ccgacgtagc gcgtcgcgcc ctcgcgcacc
     6961 cgcgcgccga ggtcttcgac cacgccgagg tgatagaggg agtcgagcag ccggaacgtg
     7021 ttgccgccgc ccatcatcac gacctcggcc tcgaggatcg cccggtccgg tgcgtccgcg
     7081 cggtgggcgg agacagcctc gatgccgaag gatccgagcg cggcgatggc cctgtccgcg
     7141 tagtcgtccc agtccgcgag cgcgtaaggg acgaacgtca cgacgccgcc ggccgagacg
     7201 ccggcgaagg cctcggccgc atgcgagaac atcgtgccgc cgtagttggt ggagttcgag
     7261 agcaggagga gctccatgtc cgccgatctg gtcggccggc ccctgaggcg atcacgagag
     7321 caagctgtgg ccgagcccgc ggacggatac gctgttccgc ggccgggagg atcatccatg
     7381 tcgggcgccg tcgcgcccgc gcgcagggta gtgcctagag cgcggtcacc tgggtcgcct
     7441 cggccttgcc cttctggccc acggcttccg tgaaggacac ggcctgaccc tcggtgaggt
     7501 tcttcacgtc gccctggatc tcggagatgt ggacgaacag gtcgtcgccg ccctcacgct
     7561 cgatgaagcc gtagcccttg tctgcgttga accacttgac tttgccttct gccatttcag
     7621 tctcatcctc tcgccccgcc ggggcacaca tataagaaaa cgacgtgacc ttcaccgcca
     7681 gagtgcggtg cgtcacgtcg caatcccacg agccggagtg gctcgcctgc taaaaatata
     7741 gcacgtttcg gcggcgagtg cggcgccgac cgttggaccg agcggccgcg acgaagacct
     7801 acggggcgag cacgacccgg cccatgacgc gcaggtagtg ggcccagtag ccttgcaggt
     7861 cgccgtagtg gtcggcgcgg cgctgcagat cgtcggccgg cagcggctcg ccgccgccgt
     7921 agacgtggcc cgcgatctgc atgagcgagc ggtcgccgta cgggatgcgc tccatgcgcc
     7981 ccaggctgcg gatcatgatg agcgccgccg accaggcgcc caccccgggg atggcgcgga
     8041 gccagcgcga cacctcgtcg taggatccct cccggaagaa ggcctcgtcc acctggctga
     8101 aggcgaccgc ggcgccgtgg atgtactcgg ctcgccgccg gttgcgcagc agtcgcgtga
     8161 ggtcctcggc gctcgcctgc acgaccgacc tggcgcccgg gaagggccag aactccctgc
     8221 cgtcgagctc cacgccggcc gagaactcct ggatcagggt gcgcttcatg tgctgcgcga
     8281 cgcccagcag gttgcgctgc gagaggatcg tccagcacgc gctctcgaac ggcgtgagga
     8341 acttgacctg gtgatagccg tagagctccc gcttgacggc cacgaagggc ggatcggagt
     8401 cgatcagcgc gtagaagcgg ctgaggtcgt cgtagacgct gaggtagacc tcgatgcgcc
     8461 gcgcgagcgc cgcgacgtcg tcctcgctca ggggctcgtc cgtgtgcagc tcgtagcgca
     8521 gccccggctc ctccacggtg cctgtcgcgg agagcttgaa ggcgaccggt gtctggctgg
     8581 cccaggccgc gcgcgtgagc gacttctccg agaccacctg ctcgccgtgc atgggcggga
     8641 acgtcacgat gaagtcgagc gcctggtcga agtcgaaggg ggccaccggt cgcagcgtgc
     8701 cttcccgtcg cagtcgcgcc accgcttcac ctccgtttct cgatgcccgg cacctaccct
     8761 gtgccggtcc gcggctaacg cttgccggac ccgaggcgcg gcgtatgagg acccttggcc
     8821 cccggcgccc tcagcttggg cagagggccc ggaccttggc cagcagcacc tcggggacga
     8881 ccggcttctc gacgaactcc tgcacgggga gccacgcgtc gtccaggtcc ttgttggaga
     8941 acttgccgac gtagatcccc gactcgttga cggcggtgag catgaggcgc ggcaaggcct
     9001 tggtgcgctc gtcggcctga agggcacgcg ccatcttgaa cccggcggcg gtgtcctcgg
     9061 ggagcatcac gtcgaggatg accaggtcgg cgccgaactc caccacgttg tcgacgacgt
     9121 ccttgtcgtc gttctgggac ccgaccacgt agccgttgct ctcgaggacc agggagaccg
     9181 tctccacgat actcggttca tcgtcgatga cgtagactcg cttcacgact tacctccatt
     9241 tgcgtgtcgg gcccgggctg tggccggccc atggtcaagc tccaacgggg acaggacatg
     9301 gcgggacagg aaaagatcgt cgtcgcagtg atgggcgcgg gagcccaagg tgttgcgact
     9361 ctcaaggcgc tgctcgcggc gccggatgtg gaggtccgct acgtggtcga cccggacctc
     9421 cgggcccccg gcgtcgcgct ggcgcgcgag cacgggatcc gctgcgagac ccacgccggc
     9481 gtcgtgaccg ccgacgccgc ggtcgacctc atcctcgaga ccggcgggga tcgagtccgt
     9541 gcggacctcg agccgggcga acaccccgac ggccgtctga tgagccccgc ggggacgcgc
     9601 ctcgtggcaa gcctcgtcgc cgagatcgcc gccgtcgagg aggacgcgcg cgtcgagacg
     9661 gcccgctacc tcaggcaggc ctcccaccag gtcaagtcgc ccctcaccgc gatccagagc
     9721 tcggtcaacg tgatcctcgg cggctacacg ggggagatcc ccgagcgcac gcgagagacc
     9781 gtgcagaaga tccacgcgcg catcgacgcg gcactcgccg cgctggccaa gcggcggctg
     9841 ctcgccgacc tgcgctgcgt cgaccgtgac gcgctcgggt ccgggaccgc ctccgtgacc
     9901 gaagcgctcc aggaggcggt cgcccgccac gccgagaccg ccgccgcgcg cggcgtcgag
     9961 ctccgcgtcc cgaccggcat cgagcccgac cgcgtgcgct gcgtgcccga gcacctcgtg
    10021 gcgctcttct cggagctggt ggagaacgcg gtggtctact cgagagacgg cggcgtcgtg
    10081 gaggtgtccg ccgacgtacg gccggacggc cggctggccg tcgccgtccg ggaccacggc
    10141 atcggcgtcc ccgagcgctg cctcccgaag atcttcgacg aggactaccg ggccgacgtc
    10201 gccgtgaagc actacgcgga cggcgccgga ctggggctcg ccgtcgcccg cgagatcgcc
    10261 ggcctgcacg ggttcgacct cgaggtgcgc agcgacgaag ggagcggcag cgtcttcacg
    10321 gcgatcatgt cgctcgcgta ggcgaggctg agggcgagcc gcgttcacag ccccgccagg
    10381 cgctcgatcg ccgccgctgc gtactccggc acacgctcgg ctagacatcc ggacaagtcg
    10441 atgctgctct ccatctccaa gggctcgatg cccatgatgg cgacgggagg gatgggatag
    10501 ccggccccac gggccatctc cagcaccttc agcacgtcgc cctcgtgggt cgtcaggccg
    10561 ctcagctcct tccgtgtctc cacttcgtcg ggcgagaaga acaggtaatc gccgggggcg
    10621 agacccagtc gcgccgcgtc gaccaccagc accgcctccg tctcggcgct caggtacgcc
    10681 accaggctga gcgcgtcggt ggagaggtcg acggcctcga aaccgcggtt gaggccgttg
    10741 accgtgacgt gctccaccac gcgcgggccg acggcgtcgt cgcccgccat gtagctgcct
    10801 acaccgacga gatagcgcac ctggtcagcg aacgaacttg acgtccacgt agtgcgtcga
    10861 gcacgagatg caggggtcgt aggacctgac cagcatctcc agcgccagct ccatctcctt
    10921 ctcgctcttg tcctgtgcga cgagctccgg tacgagcttc tcgaagtcgt actggatgtt
    10981 gttgtggttc tggttggtcg ggatgatgca gttgccgctc acgcacgtgc ccgcgtcgtc
    11041 gtactcgtac tcgtggaaga ggatgccgcg cggcacctcc gtggcgccgg cgcccgaacc
    11101 gtagcgggtg ggctccacga ggggctcgag ctcgacgccg gtcgcgatga gctcgtcgag
    11161 gagggccagc gactcgtaca cgtcggagac gatctcgacc gcctgggcga ccgtgttcat
    11221 gtaggggttg gtgcagatcg gctgcatgcc gagcgactcg gcgaccttct tggcctctgg
    11281 atgcagcctg tcgtagttgt tgttgaagcg cgcgagggcg cccgccgcgt aggaatcgag
    11341 cttgttcttg gtgtacttgg cggtcgactg cggcacgacg aactcgttgg tcacctgctt
    11401 gtactcggcg accgggtagc gctgcgtggt gccgtccggc agcaacgact ggatgtcgcc
    11461 gtcgtagagg ccgtagtcct ggtcgttgga gagcgcgatg tactccgtgg gccggtcgaa
    11521 caccggcacg acgggggcca gcgcggcgac cgtcgcgacc gccgcctcca gatccgggac
    11581 ggcggcgagg agctgctcgc gcagcgcctt gagctccttg acggtcggca ccgcggccat
    11641 gccgccgggc acgacgctca tcgggtgcgt ggtgcgcccg ccgatgagcg agccccactc
    11701 gtgcgccagc cgcttgaggc gcagcgcacg cgccaccacg tcggcgtgcg tctccacgag
    11761 cgggaagacg gacggcgcgc cgagcaggtc gggagccacg aggatgttga tgtggagcac
    11821 gtgtgagtcg aagttctcgg cgtgcttgag cagttcgcgg agcttccagc tctggctgct
    11881 gacctcgatg cccagggcgt cctcggtggc cttcaccgag gcgagcgtgt ggcccacgga
    11941 gcagatgccg cagatgcggc tggtgatgcg ggcgacctca gagtagtggc gcccgcgcac
    12001 catggactcg aagaagcgcg gggcctcggg cacctgccac tcgcacttct cgaccacgcc
    12061 gtcggtcacg ttgacgacga tgttgccgtg gccctcgacg cgggtcagat ggtcgacgcg
    12121 gatacgagca gtcgtgctca tgacgtagcc tccaggtcat tcgctgtgaa catgagcgcc
    12181 ttctcgaccg cccgcttctg cgagaacccg gcacgctgca ccagcacccg ctcgagggcg
    12241 ggcacgttgg gatgatcgac gaacccgcgg caggcctcgc aggggatacc gtaggcgggg
    12301 cagggagcgc cgcaccctgc gcgggccacc atgcccatgc agtggtctcc ctcggcgtag
    12361 cgacagaccg actcccgccg cttgcactcc acgcagaccg gatagttggg gatgaccggc
    12421 gccttgccgt gcaggaggtg gaagacgatc tgcagcactt cgtacttgtt gacggggcag
    12481 ccgtacgccg agtagtcgac cttgatggcc gcggagatcg gccgcgcgtc ctgggagtcc
    12541 aggtgcggct ggccggcgtc gtcgccgtac acgtactgct tgtagtcgtc ctgatggttc
    12601 ttcagggcgt tgatgccgcc gctggccgcg cactggccgt aggcgatgac cacggcggcg
    12661 cgctcgcgga tgctctcgag ccgcgcgcgg tcggcctcgc gtgagaagct cccctcgacg
    12721 agggcgatgt cgatgggctc tgtggtcttc tccgacatcg cctcacggaa ctcgacgaca
    12781 tcgacgtgct tgatgagctc gaggaacgcc gcgtcaccgt agttggtgaa ctcgatctgg
    12841 caaccctcgc agcaggtgaa atcgaagaat gcgaccttcg gcttgcccat cagaacgcct
    12901 cctcaagacc ctggacgtcc gcgaacgaga agacggggcc gtcgacacag acgtacttgt
    12961 cgttgatctg gcagtgaccg cacttcccca cgccgcactt catgcggcgt tcgaggtcga
    13021 cgaagacgtt ctcccgggag acgccgatct ggtccagctc gcggatcacg aagcggtaga
    13081 agatcggcgg cccgcagatg accgcgcgcg tctcgggctt gagctcgacc ttggagaaca
    13141 gcgtggtgat gacgccgacg ttgcccgccc agccctcgtc gcccttgtcg acggtctcgt
    13201 ggaactcgac gtcgtgggtg gtgcgccact tctcgaggtc gtccgtgaac agctgcagct
    13261 tgggctcgcg ggcgccgtag aagaggatga agcgcttgaa ctcgccgcgc ctgtcgagga
    13321 tgtactggat caggctgcgg gtgggacaca ggccgatccc gccggccacg atcagcacgt
    13381 cctgcccgcg caggctctcg aggtcgaagc cgttgcccag agggccgcgc acgaacacgg
    13441 gatcgccctt ctccagcccg cagacggcgc cggagacgcg cccgaccgag cgcaccacga
    13501 ggtcgaacgt gtgcttgcgc gtcggggagc tggcgaagcc gagggggatc tcgccgtagc
    13561 ccggcagccc cagctcgacg atctggccgg gctcgtactc catgggctgg tcgtcggcca
    13621 tctgcagcgt gaagtgcgtc tccgtgggcg tcacctgcac gctgcgcacg acggtggcct
    13681 cgcgggggag gaagatgttc tcgacctccg tccagtgaca ctggtgatcc atctctacgc
    13741 tcctcacgag gccatgatct gatcgatgat gtgcaccggg tcggcgatgt cggccgtgca
    13801 gccggcggaa cagcgaccgc acccgacgca ggcgggccct cccagcttct cgttgaggta
    13861 cgtcgccttg cgcatcatgc ggtggcggaa gcgcgcgccc cgctcgtcgc ggaagttctc
    13921 gcaggcgccc tcgccgagcg agcacgtggc gaagtcctcg gtgaggcagc cgtcccagta
    13981 gcgcgtgcgc tgccccgacg tctggtcgac gttccacgtg tcctgcacgt cgaagcagta
    14041 gcaggtcggg cagaccgtgt tgcaggtgcc gcaggagaag cagtcggcgg ccatggtcgc
    14101 ccacatctcg tccttgccga aggcggcgcg caccttggcg gcgatgtcgg cggtggtgta
    14161 cgccaggcgc tcgggacact gcgcaagcgc ggcggcgttg acgtcggccg cctcggtgat
    14221 ctgcgcctcc gtggccggct ggaacacacc gtgctgcagc agcgcctcgc cgcgttcggt
    14281 cagcgtctcg aacacgtagc cgtagccgat cgccgtcatg aacgcgtcgt ggccctgggg
    14341 cgccacgtcc gcgccgaccg aggcgaagaa cgcgcggctg gacaccgtct ggacgttgga
    14401 ggcgacgatc gtggtggtct ggcggtgcgc catgtagttg acgtcgaagt tgcgctccga
    14461 gaagagcagg tcggtctggt cgatggcctt gacgtcgtag aagtgcacgc cgagcaggat
    14521 cttctcgacg ggttccaggg ccgcgctgaa tcccgcctcg tcgaagcgca gcaggtcctg
    14581 cgcgggcgga aagaagagct tcttgggcgg caggatggtg acgtcgtagt cgagccgcaa
    14641 ctcctcgggc gtgtccaggc gctcgaagat gaagcgcgag cgtttggcca ccggcccgta
    14701 gaccggccca gcctcgatca gggcggtcac gaatgcgggg aagtcgtcac tcctgatgaa
    14761 cgagacagtc atgggacctc tctctgttgg atggacacct ctctcccttc ggacgagcga
    14821 ccagacgcgg caacggggcc gtcgccacga gaacatcaag tacccgaaag gctactcaaa
    14881 tgcgaacgcg ttcacaagcg cgaaactgac tgcgattagg gcgctcagcg agagagtccg
    14941 gcgggcgggc ggcggcgagc gcggccggcc ccgtagggcc gccgctcgaa gcggttgtcc
    15001 cggtaccagt cgcggccgtg ggcgagctgc cagaggctcg acagcgcgta ggccggcagg
    15061 aagaagatgc cccagcgctc gtactggcgg acgtgcacgc gctcgtgccc gcggagcgcc
    15121 gagagccacg ccggactggc gccgaggacg acgtggccga gtgtcacggc gccgtagggc
    15181 cgggagccgc gccggccgcc gagcacgcgc gcgacggcgg cgccgtggaa ctcggccgtg
    15241 cccgagacga accgcacccg gccgccgaga cagaggacgg ccagcccggc cgcgcctccg
    15301 agcgcggtgt tgggcgccgc ccacgcgtag gcggcggcgc gagacacgag gcgacgggcg
    15361 ttcataccgg ccggccgttc gccggaggcc ggcgccggct cagcgcgctc gcttggatcg
    15421 tgcgtcgcgc tcgtcagccg gcgggttcgc ggatcatcac cagcatcatc ttgaagcgct
    15481 cgaccgcgtg cagggcgtga ggcacgttgg ccggcatcac gatcagctcg tccttgcgca
    15541 ccgtgtgtgg cgtgccgccg atggtgatct cggccacgcc gtctagcacg tggacgagcg
    15601 cgtcgaaggg cgcggtgtgc tcgctcaggc cctctccctt gtcgaaggcg aacacgctca
    15661 ccgtgccggt cttcttcttg atgatctccc ggctcaccac tcctccggcc tggtagtccg
    15721 cgagctcgag cgcgttaccg ccggtgaacc gcccctcgct gctgtcttcc tgcgaaccag
    15781 gcatgggccc gtcctctccc tcgattggtc tctcgctgca cactaccccg gcggccaagg
    15841 gaggtcacca tgagtcgctg cggtcgcgct caccgctctc gcgctcgcca ctcggagagc
    15901 ccgttcctca tgaaaggcca gcctcgtcaa cgctggcgtg ctaccaccgt tcagcataat
    15961 gtagacacgt gtgtactctg ccgacgcggg ggccatgagc gacgcaacgc tgcccagctg
    16021 gaacgaccga cctgcaaagg ccgcgatcct cgagttcgtc cgctcggtga cgacacccgg
    16081 agcctcgtac gtacctgtgg ccgagcggat cgccgccttc gacaacgacg gcaccctctg
    16141 gtgcgagaag cccatgtacg tccaggccga cttcgtctct cgtcgctgga aggacatggt
    16201 cagggaagat ccgcgtcgag cgaccgagca gccctacaag gcgctcatcg agggcgacgc
    16261 cgaatggttt tcggacgtct acgcccacgc gccggagctg gtcaagggtg tcgccgaggc
    16321 ctacgagggc atcaccacgg aggccttcga cgcggcggca cgggagttct tcgcatccgc
    16381 cacgcatccg accctcggcg tcccgtatgc cgacaccgcc taccggccga tgcgcgagct
    16441 gatcggactg ctcgaagcga acgactttca cgtctacatc tgctcggcgg gcggccgcga
    16501 cttcgtacgt gctgtgagtg agcggatcta cggcctgcct caggaccgcg tgatcggctc
    16561 ggcggcgacc ctggagtatc gcgacggcga cctgtaccgc acggcgggcg tcgagcagcc
    16621 catcgacgac gggcccggca aggtcgtcca catctggacg cgcacgggac gcaagcccct
    16681 gctcgccggg ggcaacgccg acggcgatgt cccgatgctg gagacggccc gcttcgcgct
    16741 cctggtgcac cacgatgacg cggagcgcga gttcgcctac gaccaagggg cggagcaagc
    16801 gctcgccgca gcagctgccg gcggttggac cgtggtcagc gtgaaggagg acttcaggac
    16861 ggtcttctga ccggcttgac cggggcgggc gcaggagccg acccgcgcac gacactggag
    16921 gggtgatggg aatgggtgag ccgttcaagg gcgtcatcaa cgtcgacatc cgcgactcga
    16981 cgccggactg ggcgccgttc gagccgccca aggcgccgga cgacgccccc aacgtcgtct
    17041 acatcgtgct cgacgacgtc ggcttctcgg ccatgagctg ctacggaggg ccgatcgaga
    17101 cgcccaacat cgaccgcctc gccgccgagg gcgtgcgcta cacgcagtgg cacacgaccg
    17161 ccctctgctc accgacacga tcgtgcctgc tgacgggccg caaccacacc cgcaacagca
    17221 tggcctgcat caccgagggc gcgatcgggt tcccgaacgg gagcggcacc gtcccgcccg
    17281 agaacggcat gctctccgag atcctcggcg agttgggctg gaacacctac atggtcggca
    17341 agtggcacct ctgcccgacg gacgagatga acctggcggc gccgcgccgc aactggccga
    17401 gcgggcgcgg attcgaacgc tggtacgggt tcctgggggc ggagaccaac cagtggtatc
    17461 cggagctcgt gtacgacaac catccggtgg accagccgaa gtcgccgcag gagggctacc
    17521 acctcaccga ggacatcacc gacaaggcgc tcgagttcat caaggacgcc cacgccatcg
    17581 ctcccgacaa gccgttcttc ctctactacg ccccgggggc gtgtcacgca ccgcaccatg
    17641 cgcccaggga gtggatcgac aagttcaagg ggcgcttcga cgcggggtac gaggcgatgc
    17701 gggtcgagac gctcgcccgg cagaaggaga tggggctcgt cccgcccgat accgagctgc
    17761 cgccggtgaa tccgatcggc acatcggcga cgcgcaaggg acccaagggg cagcccttcc
    17821 cgggattgga cctcacgaag ccgtgggact cgttgtctga tgaagagagg cggctcttcg
    17881 cgcggatggc cgaggtctac gccggcttcc tcgcccacgc ggaccaccac atcgggagac
    17941 tgctcgacta cctggacgaa gccgaactgc gcgagaacac cctggtcatc gtggtctccg
    18001 acaacggagc cagcggcgag ggcggtcccg tcggctcggt gaacgagaac ctgttcgtga
    18061 acggcatgcc cgacgatctc cagaccaacc tcgcgctgct cgacgaactc ggcggcgtca
    18121 agacctacaa ccactacccg accggctggg cgatggcctt caacacgccg ttcaagatgt
    18181 ggaagcgcta caacttcaac ggcggcacct cggacccatg cgtcatctcc tggccggcgg
    18241 ggatgaag
//
LOCUS       DASUMX010000066         7162 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1455603, whole genome shotgun sequence.
ACCESSION   DASUMX010000066 DASUMX010000000
VERSION     DASUMX010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 7162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7162
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1455603"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            178..399
                     /locus_tag="VFZ86_02785"
     CDS             178..399
                     /locus_tag="VFZ86_02785"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HEX5641246.1"
                     /translation="MTPQHPEDGKKKDQPRSAASAESVAFLLLAGVAVGLGFGAGVDW
                     LFGTFPLFVVIGVFVGFALALYAVYLETK"
     gene            480..800
                     /locus_tag="VFZ86_02790"
     CDS             480..800
                     /locus_tag="VFZ86_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641247.1"
                     /translation="MAAFLEQPVWAAAAGAGLVLVFWALEALAWRSAARGSFNHAVLA
                     AVGGAAVRMGIVLVCLVLIGVFARPAFPTAALSFLAAFTVYLAVRLVAFSGEPATPRG
                     VGTR"
     gene            797..1660
                     /gene="atpB"
                     /locus_tag="VFZ86_02795"
     CDS             797..1660
                     /gene="atpB"
                     /locus_tag="VFZ86_02795"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEX5641248.1"
                     /translation="MKRKRLIWASLVIVLLAALIGYDFLHERGESQEFGIAEEFELHP
                     IIELPAIGPFDLSITKAVVYLWITTGVLIVFALLVRRSLKRPTGRFRVMIELLYALAH
                     DGIVGAVMKSGRATWFPFIAGAFFFVLTANLIGLIPLPFAEHHQLGFYTATANLYVTL
                     SLALCTFVFTHYAGIRQNGGLHYFVNWAPPSAPPVLKQLIWVIHLVSEVFRLVSLAVC
                     LFANMLAGHAILAVFFAMALIFQSYLAAVVLQGASVLIYLFEIFVAFIQAFIFAILSA
                     VYIAGALDQDH"
     gene            1712..1942
                     /gene="atpE"
                     /locus_tag="VFZ86_02800"
     CDS             1712..1942
                     /gene="atpE"
                     /locus_tag="VFZ86_02800"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEX5641249.1"
                     /translation="MDAEAAKLLGAGIAMGVGALGPGIGLGMLLGKAYESIARQPEVA
                     GDIRTSMFIGIGVTEAVALYAFVVALILIFVA"
     gene            1978..2487
                     /gene="atpF"
                     /locus_tag="VFZ86_02805"
     CDS             1978..2487
                     /gene="atpF"
                     /locus_tag="VFZ86_02805"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEX5641250.1"
                     /translation="MLVPEPGLMIWTLITFGVAVLVLTLFAFKPLQKALDERRQHIQD
                     SVDAAEETRAEALRLLEEYKATLAKVRGESEEILERSRTTAEHAKAEIMADARAQSQR
                     VLEKAHEQIERDTRAALREIKGQIAELTALATEKVAASSLSAADQQRLIDETLAELDV
                     EKLGAEGKA"
     gene            2484..3038
                     /gene="atpH"
                     /locus_tag="VFZ86_02810"
     CDS             2484..3038
                     /gene="atpH"
                     /locus_tag="VFZ86_02810"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HEX5641251.1"
                     /translation="MSEEQVARVYAQALFDAASDAGVVEPVRRELGDFVAALAASASL
                     RDVLADPRVETAAKHRVLAEITRGGQQLLANALQLMLERGRFAAVPDLHQAYDALALV
                     DEGVVEVEVVSAAELSPDTQKKIADRVREVTGRRVELARRVDPSILGGLVLRMGDIIV
                     DGSVKARIRQLRRKLATAEVRGDV"
     gene            3038..4633
                     /gene="atpA"
                     /locus_tag="VFZ86_02815"
     CDS             3038..4633
                     /gene="atpA"
                     /locus_tag="VFZ86_02815"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEX5641252.1"
                     /translation="MKLRPEEIASVLKAQIQDYQAEVEVEEVGTVLEVGDGIARVYGL
                     RNCLAMEMLELPHDVIGLALNLEEDNVGAVLLGEDTLIKEGDLVKRTGRVLQVPVGEA
                     MIGRVVDPLGRPLDDKGPIETDQTRPVEFKAPGVIERQGVKEPLQTGIKAIDSMIPIG
                     RGQRELIIGDRQTGKTSLIVDTIINQKGQDEICVYVAIGQKASTVMQVVQKLEEYGAM
                     EYTIVVAATASASAPLKYLAPYAGCAMGEYFLYGGRHAVCFYDDLSKQADAYRQMSLL
                     LRRPPGREAFPGDVFYLHSRLLERAVKLNDELGGGSLTALPVIETQAGDVSAYIPTNV
                     ISITDGQIFLTSELFYSGIRPAINVGISVSRVGGKAQVKAMRQVAGRLKLDLAQYREL
                     QAFAQFGSDLDEVTRRTLARGERMVQLLNQGRFDPMPLEEQVAVIFAGNQGFLDDLDV
                     HRVRPFADGLRDYLRAEAEDVLADIREKGELSEETETRLKTAVTAYHNSFAADAGGRI
                     AVERMAAESEALVAKAGPAEAED"
     gene            4635..5510
                     /gene="atpG"
                     /locus_tag="VFZ86_02820"
     CDS             4635..5510
                     /gene="atpG"
                     /locus_tag="VFZ86_02820"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011879441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HEX5641253.1"
                     /translation="MATLRDIRRRIASVQNTRKITKAMEMVAAAKLRRNQARIEALRP
                     YAGDMIEMMIDLATYSEDARQYVLLQEREVHTVAVVAMTGDRGLAGAFNANVVRKAMA
                     IERDLREQGIETRVLAVGRKGIGTLKFRGCRLEKSWQGLSDRPQFTDAKAIAAELIRM
                     YVDGEVDRVRLVYNHFTSPIEQTLMDDVILPVPREVVTDGGEERPPVSYIYEPAPEDI
                     LEGLLPTYVEIAVYRALLESSASEQGARMSAMRNASDAAEDMIGGLTLALNRARQAAI
                     TQEILEVVGGAEALG"
     gene            5566..6972
                     /gene="atpD"
                     /locus_tag="VFZ86_02825"
     CDS             5566..6972
                     /gene="atpD"
                     /locus_tag="VFZ86_02825"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEX5641254.1"
                     /translation="MNTGTVVQVIGAVIDVRFPDEIPAIYNALKVRIGEGDGNEVICE
                     VQQHLGDDKVRAVAMDATDGLARGVSVIDTGQPMTVPVGEATLGRLIDVNGNTIDKGE
                     PLTDAEHWPIHRPAPSFDELDPTDELFETGIKVVDLLAPYVKGGKIGLFGGAGVGKTV
                     IVQELINNIATQHGGYSVFCGVGERTREGNDLWTEMQESGVLAKTALIFGQMNEPPGA
                     RLRVGLAGLTVAEYFREAGGQDVLLFIDNIFRFVQAGSEVSALLGRMPSAVGYQPTLA
                     NEMGELQERITSTKRGSVTSVQAIYVPADDLTDPAPAATFTHLDATTTLSRAISEKGI
                     YPAVDPLDSNSRILTRDAVGDEHYEVALRVQEILQRYKELQDIIAILGMDELSDEDKV
                     VVQRARKIERFLSQPFTVAEAFTGTPGKYVPLADTVRGFKDLVEGKCDELPEQAFFMV
                     GGIEEAAEKAKKLSGADA"
     gene            6983..>7162
                     /gene="atpC"
                     /locus_tag="VFZ86_02830"
     CDS             6983..>7162
                     /gene="atpC"
                     /locus_tag="VFZ86_02830"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010899878.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the epsilon subunit is part
                     of the catalytic core of the ATP synthase complex; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEX5641255.1"
                     /translation="MSDERTLQVEVVTPDGQVYAGDVAMVVLPGVDGELGILPRHQPL
                     VTLLAIGETRVRTVTD"
BASE COUNT         1143 a         2566 c         2382 g         1071 t
ORIGIN      
        1 cggctgctca gccgctaccg gtcgcgccgc cgggggctgc ccgaccctct ggcgccgggt
       61 gtcgggaaca accccacggg ggcgggcggc gccggccgcg catgaaccgc cgccccctca
      121 atgcgaaaaa tggtatctgt atcccatttg caggtgcagg ggtcaggcag gtatagtttg
      181 acgccacagc accccgagga cgggaagaag aaggaccagc cgcgttcggc ggcgtccgcg
      241 gagtccgtgg cgttcctcct gctcgccggc gtcgccgtcg ggctcgggtt cggcgccggc
      301 gtcgactggc tcttcggcac gtttcctctc ttcgtggtga tcggcgtgtt cgtcggcttc
      361 gcgctggcgc tctacgccgt ctatctggag acgaagtgac gggttcagga gggcgaggga
      421 ttcgcaggtc actcgtagcg ccgctgcttc tggcagcggc cgttctggcg tgcgccactg
      481 tggccgcgtt cctcgagcag ccggtgtggg ccgctgcggc gggggccggc ctcgtgctcg
      541 tcttctgggc gctcgaggcg ctcgcctggc gctccgccgc gcgcggcagc ttcaaccacg
      601 ccgtgctcgc ggccgtcggc ggggccgccg tgcgcatggg gatcgtcctc gtgtgcctcg
      661 tgctcatcgg cgtgttcgcg cggccggcgt tcccgacggc ggcgctctcg ttcctcgccg
      721 cgttcaccgt gtacctggcc gtgcgcctgg tcgccttctc cggtgagccg gcgacgccgc
      781 ggggggtggg cacgcgatga agcgcaagcg cctgatctgg gcgagcctcg tcatcgtgct
      841 gctggccgcg ctcatcggct atgacttcct gcacgagcgc ggcgagagcc aggagttcgg
      901 catcgccgag gagttcgagc ttcacccgat catcgaactg ccggccatcg gcccgttcga
      961 cctctcgatc accaaggcgg tcgtctacct gtggatcacg accggcgtcc tcatcgtctt
     1021 cgccctgctc gtccgccgca gcctgaagcg cccgacgggc cgcttccgcg tgatgatcga
     1081 gctgctctac gccctggctc acgacggcat cgtcggggcg gtcatgaaga gcggccgcgc
     1141 cacctggttc cccttcatcg cgggcgcgtt cttcttcgtg ctcacggcga acctcatcgg
     1201 gctcatcccg ctgccgttcg ccgaacacca ccagctcggc ttctacacgg cgaccgccaa
     1261 cctgtacgtc accctgtcgc tggcgctctg caccttcgtc ttcacccact acgccggcat
     1321 ccgtcagaac gggggtcttc actacttcgt gaactgggcg ccgccgagcg cgccgccggt
     1381 cctcaagcag ctgatctggg tgatccacct cgtcagcgag gtcttccgcc tggtctcgct
     1441 cgccgtctgt ctcttcgcca acatgctggc ggggcacgcc atcctcgccg tcttcttcgc
     1501 catggcgctg atcttccaga gctacctcgc ggccgtcgtc ctgcagggcg cgtcggtcct
     1561 catctacctg ttcgagatct tcgtggcctt catccaggcc ttcatcttcg ccatcctctc
     1621 cgccgtgtac atcgccggag cgctggacca ggaccactag cgatgggcgc agccatcctg
     1681 ccgcatcgca gaccgtcaag gaggaacgtg aatggacgct gaagccgcca agttgctggg
     1741 cgccggtatc gccatgggcg tgggagccct cggccccggc atcggcctgg gcatgctgct
     1801 gggcaaggcc tacgagagca tcgcccgcca acccgaggtc gccggtgaca tccgcaccag
     1861 catgttcatc ggtatcggcg tgaccgaggc ggtggcgctg tacgccttcg tggtcgccct
     1921 gatcctcatc ttcgtcgcct gagcagggcc cggtaccgaa ccagcgagga gtgacacatg
     1981 ctcgtccctg aaccgggtct catgatctgg accctcatca ccttcggggt ggcggtcctc
     2041 gtgctcacgc tgttcgcctt caagccgctc cagaaggctc tcgacgaacg gcgccagcac
     2101 atccaggaca gtgtcgacgc cgccgaggag acgcgcgccg aggcgctgcg cctgctcgag
     2161 gagtacaagg ccacgctcgc caaggtgcgc ggcgagtcgg aggagatcct cgaacgctcg
     2221 cgcaccaccg ccgagcacgc caaggccgag atcatggccg acgccagggc gcagtcccag
     2281 cgcgtcctcg agaaggcgca cgagcagatc gagcgcgaca cgcgcgccgc gctgcgcgag
     2341 atcaagggcc agatcgccga gctcaccgcg ctcgccaccg agaaggtggc ggcgagcagc
     2401 ctgagcgcgg ccgaccagca gcgcctgatc gacgagacgc tcgcggaact cgacgtcgag
     2461 aagctcggcg cggagggcaa ggcgtgagcg aggagcaggt cgctcgggtc tacgcccagg
     2521 ccctgttcga cgccgcgagc gacgccggcg tggtcgagcc ggtgcgccgc gagctgggcg
     2581 acttcgtcgc cgccctcgcg gcgtccgcgt cgctgcgcga cgtgctcgcc gacccccggg
     2641 tcgagaccgc cgcgaagcac cgcgtgctcg cggagatcac gcgcggcggc cagcagctgc
     2701 tcgccaacgc cctgcagctc atgctcgagc gcggtcgttt cgccgcggtg ccggatctgc
     2761 accaggccta cgatgccctc gcgctcgtcg acgagggcgt ggtcgaggtc gaggtggtct
     2821 cggccgccga gctgtcgccc gacacccaga agaagatcgc cgaccgtgtc cgggaggtca
     2881 ccgggcgccg ggtcgagctg gcccgccgcg tcgacccctc catcctcggc ggcctcgtcc
     2941 tgcgcatggg cgacatcatc gtcgacggca gcgtcaaagc acggatccgc cagctgcgcc
     3001 gcaagctggc taccgcagaa gtgagaggtg acgtctagtg aagctgcgac ccgaagagat
     3061 cgcctcggtc ctcaaggccc agatacagga ctaccaggcc gaggtcgagg tcgaagaggt
     3121 cgggaccgtc ctcgaggtcg gcgacgggat cgctcgtgtc tacggcctgc gcaactgcct
     3181 ggccatggag atgctcgagc tccctcacga cgtcatcggc ctggccctca acctcgaaga
     3241 agacaacgtc ggcgccgtgc tcctcggcga ggacacgctc atcaaggagg gcgacctcgt
     3301 caagcgcacc ggccgcgtgc tccaggtccc cgtcggcgag gccatgatcg gccgcgtcgt
     3361 cgacccgctc ggccgtccgc tcgacgacaa gggccccatc gagaccgacc agacccgccc
     3421 ggtcgagttc aaggccccgg gcgtcatcga gcgtcaggga gtcaaggagc cgctgcagac
     3481 cggcatcaag gccatcgact ccatgatccc gatcggccgc ggccagcgcg agctcatcat
     3541 cggcgaccgc cagaccggca agacctcgct gatcgtcgac acgatcatca accagaaggg
     3601 ccaggacgag atctgcgtct acgtggccat cggccagaag gcctccacgg tcatgcaggt
     3661 cgtgcagaag ctcgaagaat acggcgccat ggagtacacg atcgtcgtcg ccgccaccgc
     3721 ctcggcctct gcaccgctca agtacctcgc cccctacgcg gggtgcgcca tgggcgagta
     3781 cttcctctac ggcggccggc acgcggtgtg cttctacgac gacctctcca agcaggccga
     3841 cgcctaccgc cagatgtcgc tgctgctgcg ccgcccgccg ggccgcgaag cgttcccggg
     3901 cgacgtgttc tacctgcact cccgcctcct cgagcgcgcc gtcaagctca acgacgagct
     3961 cgggggaggc tcgctcacgg cgctgccggt catcgagacg caggccggcg acgtgtccgc
     4021 ctacatcccg accaacgtca tctccatcac cgacggccag atcttcctca ccagcgagct
     4081 cttctactcc ggcatccgcc cggccatcaa cgtcggcatc tccgtgagcc gggtgggcgg
     4141 caaggcgcag gtcaaggcca tgcgccaggt cgcgggccgc ctcaagctcg acctcgccca
     4201 gtaccgcgag ctgcaggcct tcgcccagtt cggctcggac ctcgatgagg tcaccaggag
     4261 gacgctggcg cgcggcgagc gcatggtcca gctgctcaac cagggccgtt tcgaccccat
     4321 gcctctggag gaacaggtcg ccgtcatctt cgccggcaac caggggttcc tcgacgacct
     4381 cgacgtccac agggtgcggc cgttcgccga cggcctgcgc gactacctgc gcgccgaggc
     4441 cgaggatgtg ctggccgaca tccgcgagaa gggcgagctc agcgaggaga ccgagaccag
     4501 gctcaagacc gccgtgaccg cctaccacaa ctcattcgcg gcggacgccg gcgggcggat
     4561 cgccgtcgag cgcatggccg cggagtcgga ggcgctcgtg gcgaaggccg gacccgcgga
     4621 ggcggaggac tgacgtggcg acgctgcgcg acatccgccg gcgcatcgcg tcggtccaga
     4681 acacgcgcaa gatcaccaag gccatggaga tggtcgccgc cgccaagctg cgccgcaacc
     4741 aggcgcgtat cgaggcgctg cgcccgtacg ccggcgacat gatcgagatg atgatcgacc
     4801 tcgccaccta ctcggaggac gcgcggcagt acgtgctgct ccaggagcgc gaggtgcaca
     4861 cggtcgccgt cgtcgccatg accggcgacc gcggtctggc cggcgccttc aacgccaacg
     4921 tcgtgcgcaa ggcgatggcg atcgagcgcg acctgcgcga gcagggcatc gagacgcgcg
     4981 tgctcgccgt cggccgcaag ggcatcggga cgctcaagtt ccgcggctgc cggctggaga
     5041 agagctggca gggcctcagc gaccggccgc agttcaccga cgccaaggcg atcgccgccg
     5101 agctcatcag gatgtacgtg gacggcgagg tcgaccgcgt gcgcctcgtc tacaaccact
     5161 tcacgtcgcc catcgagcag acgctcatgg acgacgtgat cctgccggta cccagggagg
     5221 tggtcaccga cggcggtgag gagcgtcccc cggtctcgta catctacgag cccgcgccgg
     5281 aggacatcct cgagggcctg ctgcccacct acgtcgagat cgccgtgtac cgggcgctcc
     5341 tcgagtcgag cgccagcgag cagggcgcgc gcatgtcggc gatgcgcaac gccagcgacg
     5401 cggccgagga catgatcggc ggcctcacgc tggcgctcaa ccgcgcccgc caggcggcga
     5461 tcacccaaga gatcctcgaa gtcgtcggcg gcgccgaggc gctgggatga agaccaaggc
     5521 taccgcccga tccaccgacc agacacgtga agcggagttg acgcgatgaa cacaggcaca
     5581 gtcgtccagg tgatcggggc ggtcatcgac gtccggttcc cggacgagat cccggccatc
     5641 tacaacgccc tcaaggtccg catcggcgag ggcgacggca acgaggtgat ctgcgaggtg
     5701 cagcagcacc tcggcgacga caaggtccgc gccgtcgcca tggacgcaac ggacggcctc
     5761 gccaggggcg tctcggtgat cgacaccggg cagccgatga cggtgcccgt cggtgaggcc
     5821 acgctgggcc ggctcatcga cgtcaacggc aacaccatcg acaagggcga gccgctcacc
     5881 gacgcggagc actggccgat ccatcggccg gcgccgtcgt tcgacgaact ggatcccacc
     5941 gacgagctgt tcgagaccgg catcaaggtc gtcgacctgc tcgcccccta cgtcaagggc
     6001 ggcaagatcg gcctcttcgg cggcgccggc gtgggtaaga ccgtcatcgt gcaggagctc
     6061 atcaacaaca tcgccacgca gcacggcggg tactcggtgt tctgcggcgt gggcgagcgc
     6121 acgcgcgagg gcaacgacct ctggaccgag atgcaggagt ccggggtcct cgccaagacc
     6181 gccctgatct tcggccagat gaacgagccg ccgggcgcgc gcctccgcgt cggcctggcc
     6241 ggtctcaccg tggcggagta cttccgcgag gccggcggcc aggacgtgct gctcttcatc
     6301 gacaacatct tccgcttcgt ccaggcgggc tccgaggtct cggcgctcct cggccgcatg
     6361 ccgagcgcgg tcgggtacca gcccacgctc gcgaacgaga tgggcgaact ccaggagcgc
     6421 atcacctcca ccaagcgcgg ctcggtgacc tccgtgcagg ccatctacgt gccggcggac
     6481 gacctcaccg acccggcgcc ggccgccacg ttcacacacc tcgacgccac caccaccctc
     6541 tcccgcgcca tctcggagaa gggcatctac ccggccgtcg acccgctcga ctccaactcg
     6601 cgcatcctca cgcgcgacgc cgtgggcgac gagcactacg aggtggcgct ccgcgtccag
     6661 gagatcctgc agcgctacaa ggagctgcag gacatcatcg cgatcctcgg catggacgag
     6721 ctctccgacg aggacaaggt cgtcgtgcag cgcgcccgca agatcgagcg cttcctcagc
     6781 cagccgttca ccgtcgccga ggcgttcacg ggtacgccgg gcaagtacgt cccgctcgcc
     6841 gacacggtgc gcggcttcaa ggacctcgtc gagggcaagt gcgacgagct tcccgagcag
     6901 gccttcttca tggtcggcgg catcgaggag gcggccgaga aggccaagaa gctctccggc
     6961 gccgacgcgt gagaggcgtg aggtgagcga cgagcgcaca ctccaggtcg aggtcgtcac
     7021 gcccgacggc caggtgtacg ccggggacgt cgccatggtg gtgctcccgg gcgtcgacgg
     7081 cgagctcggc atcctcccgc gccaccaacc gctggtgacg ttgctggcca tcggcgagac
     7141 gcgtgtccgc acggtcacgg ac
//
LOCUS       DASUMX010000067        51395 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_737301, whole genome shotgun sequence.
ACCESSION   DASUMX010000067 DASUMX010000000
VERSION     DASUMX010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 51395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51395
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_737301"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1989)
                     /locus_tag="VFZ86_02835"
     CDS             complement(<1..1989)
                     /locus_tag="VFZ86_02835"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEX5641256.1"
                     /translation="MATARPKQARRRAAAAARTAPRGAAAASRAKTKPPASRHRRELG
                     AIACLAVAVFLAFVLYLGWDGGSLGRWLGDAARWLVGILAFALPFLLGFVAYLLVVKE
                     ESRPRRGLSWGVALIVTGVALAAAADAFGIFGGERGDALFRDAYMSAHGGVLGEVQWA
                     VLSPFIGRLGVDVLVIALILAGVLLVTGSSLRQWATHSRQGVAKAGRAARSQAEALGA
                     RRKEASDTRVVELDEAQTIDAGDLMRTSVRPAVSPELDFSRPVPAAGPHLIDGAAEAP
                     EIFGEPTPLPGEHEPEPVVAAEDGVQLSLAAAASAQAEKGAAGEADVALAFAEAERRQ
                     WTLPDPALLRRLAQGHGESPDAVARVSERLVATLGSFNIPAQVIGTVSGPRVTRYELQ
                     LEPGIKVSKVAGLKDDLKYALAATDVRVLAPIPGKTAVGVEVPNQQANYVSLGDIHGL
                     FPQHASPMAFWLGKDVTGSAVLADLVRMVHLLIAGTTGSGKSGCLNALITSILLRATP
                     DEVRMIMIDPKKVELSNFNGVPHLLAPVVTNMKQATYVLDNICREMDRRYDVLSRNGC
                     QDIRALNKKLVRAGEDPMPYTMVIIDELADLMMVAPSEVEDSVIRLGQLGRSCGIHLV
                     VATQRPSVDVVTGMIKTNIPSRIAFAVASQTDSRIILDQ"
     gene            complement(2049..3713)
                     /locus_tag="VFZ86_02840"
     CDS             complement(2049..3713)
                     /locus_tag="VFZ86_02840"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEX5641257.1"
                     /translation="MSEDAVRIVPLGGLGEIGKNMTAVEYRGKIVVLDCGVEFPSEDM
                     LGIDLVLPDIRWLVEREADVLAFVITHGHEDHQGALPFVLRRLNRPVYGSRFTLALIK
                     SKLEEHGLLKSVELTEVSDKSRLELGPFDLSFIATSHSIPDSLSIVLGTDLGAVVLTG
                     DYRFDHTPIDGRVTDVDAFARLGERGVLALLGDSTNAEAPGSTASESTVSNAFHQIFA
                     EAEGRVIVASFASHIHRIQQAITIAHLHGRKFALSGRSMVKNANIARNLGYLKVPEGA
                     MIKLTQIDEYRPEEVLILSTGSQGEPLSALTRMAFNDHPQVELHEGDTVVISAKAIPG
                     NERSVTATIDRLLKVGATVVYGERSGIHVSGHAAQEDLKMMLNLTRPRFLVPVHGEYR
                     MQHLHAELARQSGMPDEDIFILENGDVLELEADNGEIVDKVATGMVFVDGSEIGDPEG
                     LVLRDRQQLAGDGILIAVVTVDGQTGDSVAPPELVARGFLHDDARLQDVLDECGAALD
                     TLLDELGAEHVTGQHLIREDISQRLSELVFRRTKSRPLIMPIVVEV"
     gene            complement(3760..4647)
                     /gene="dapA"
                     /locus_tag="VFZ86_02845"
     CDS             complement(3760..4647)
                     /gene="dapA"
                     /locus_tag="VFZ86_02845"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEX5641258.1"
                     /translation="MKDHRLGTILTAMVTPFDAAGTVDHDKLAALAEFLLAHGSDGLV
                     VAGTTGESPTLDDREKLAMFRTVVAAVDGRAPVVAGTGSNDTRHSAELTRAAEACGVD
                     AVLAVVPYYNKPPERGVIAHFRAVAAATSLPVIVYNIPGRCVINVAPEALAELGEVDN
                     IAAVKQANPDLAETDRLMELSDLAVYAGNDDMLYDVMARGGAGGICVASHVAGPRMAE
                     VARLCREGDLEAAAAEDAALHELYKALFITANPIPVKAALNLLGHEVGGLRLPLVPAT
                     PEEEAVVAAELRRLGLTDA"
     gene            complement(4644..5441)
                     /gene="dapB"
                     /locus_tag="VFZ86_02850"
     CDS             complement(4644..5441)
                     /gene="dapB"
                     /locus_tag="VFZ86_02850"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007513439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEX5641259.1"
                     /translation="MIDVLVTGAAGKMGALSAATIAAQDDLRLVALVDPKKGRGGETP
                     WFEDVEAALALTTPAAALEFSVPGAVFENSAALLEAGVPTVVGATGLDDDQVAGLSAR
                     AEASGAGLVIVPNFALGAVLLMKFAAEAARHYEHAEIIETHDKAKADAPSGTSLRTAR
                     LMAEAPGSALAAPPGAGPASRGLVADGRVTIHSVRLPGAVAHQEVVFGGEDELLTLRH
                     DSLSRRSFMAGVLLALRRVGTVRGTVIGLEHLLDSPDAPGRAPKEDA"
     gene            complement(5491..6717)
                     /locus_tag="VFZ86_02855"
     CDS             complement(5491..6717)
                     /locus_tag="VFZ86_02855"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX5641260.1"
                     /translation="MATERLDSVRSVALGVWIAAGSRLEPVELGGISHFIEHLIFKGS
                     DKFSASQIAHIFDDMGAEPNASTSKEYTLVNARLLDEDLDKAFEVIAEMVARPSFADL
                     DQEREVVLEEVAMYEDSPPELIHDDLCEVVFGEHPLGRPIIGHYETLAALDLDTVRGY
                     HDAHYVNPGVVIAASGHVDHERVAALAATHFRPEPGAEVVRAELSAPETRHLARFTQK
                     DTEQYHVCLGGPGPRRGDPDRYAAFVVDTILGGSWSSHLFQEVREKRGLAYSVYSYTS
                     LYADAGLAAVYFGSRQEAVEEAMQVILETLAGLEAQMTDAAISRAKNHLKGQLVLSME
                     SPGARMQTLGRAVLLEQPVLTVDEVLARIDAVDRDATVAAVGRYYDPETWSTACIGPQ
                     PEPFRAVTQGFVQEER"
     gene            complement(6789..8885)
                     /locus_tag="VFZ86_02860"
     CDS             complement(6789..8885)
                     /locus_tag="VFZ86_02860"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEX5641261.1"
                     /translation="MNTIEVEVGGQTITLETGRLAKQANGAVLVRCGDTVVLVSAVMR
                     EDVREGQDFFPLTVDVEERHYAAGKIPGGFIKRESRPSEKAILAARQIDRPIRPLFPK
                     GFMNEIHVVATTLSVDFEVSYDILATLGASAALTISNIPFGGPIGSVHVGRIDGQLVV
                     NPTLEELQDESDMDLVVTGTADAIVMVEAGAQEVSEAVIVEALKLAHEEIKKLVAVQL
                     ELQKQVGKAKWDVPVTTIDEGVLEEVRTGFAAALDTATQVPGKKERMDATADVKKAAV
                     AQLLSEDSDPERVLQVKRALAKVEKDIIRDRIAVKKVRPDGRATDEVRPISCEVGVIP
                     RTHGSALFTRGETQAMSLLTLGGTGEFQRIDGLGIEDKKRYIHHYNFPPYSVGETGFM
                     RGPKRRDIGHGALAERALLPVLPSELDFPYTVRIVSEILESNGSSSMASVCGSTLSLM
                     DAGVQITAPVAGTAMGLIKEGDDYVVLTDIAGVEDHLGDMDFKVAGTAEGITALQMDI
                     KITGVTFEILTEALEQARKARLHILAKMAEVIAEPRSELSQWAPRIFSIQINPDQIGL
                     VIGKGGETIRGMTEEFSADIDIQDDGTIFICAPDEEAAEGVKNRIQSMTKEVEVGDVF
                     TGRVVKTTDFGAFVELKKGTDGLLHISRLAPKGERVKNVEDVVKRGDSVTVEVVEIDK
                     ARNRIGLTLLKPPEAE"
     gene            complement(8996..9259)
                     /gene="rpsO"
                     /locus_tag="VFZ86_02865"
     CDS             complement(8996..9259)
                     /gene="rpsO"
                     /locus_tag="VFZ86_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEX5641262.1"
                     /translation="MTKDAKETIIAKHAKHDGDTGSPEVQIALLTERITTLTEHLKTH
                     KKDHHSRRGLLKMVGQRRRLLNYLQARDLERYRALVKELELRK"
     gene            complement(9398..10375)
                     /locus_tag="VFZ86_02870"
     CDS             complement(9398..10375)
                     /locus_tag="VFZ86_02870"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEX5641263.1"
                     /translation="MEVVADIASLAPRPRALALGTFDGVHAGHRAVICRAVRLARERG
                     LTSAVVTFDRHPLAVIDPARVPRLLTSNEEKARLIAELGPDELVMLPFDEELAALTPA
                     EFCDGLLAGALRARAVVVGENFNFGARGAGDTAQLRACGAANGYEVEVCALVTEHGEP
                     ISSTRIRRLLHDGELEEVREILGRPPSAGGVVVHGDKRGRALGVPTANVDVEAGTIFP
                     GRGVYAARAFVDGRWYRTAVNIGHNPTFQRRDEPTTHVTVEAFLLGFSGDIYDREIRL
                     DFLHKIRDERRFGSAEALVAQMRLDIAAAGSLADPELDAVGLAPDAGGA"
     gene            complement(10375..11286)
                     /gene="truB"
                     /locus_tag="VFZ86_02875"
     CDS             complement(10375..11286)
                     /gene="truB"
                     /locus_tag="VFZ86_02875"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEX5641264.1"
                     /translation="MAQFRTRKAPLDGVLLIDKPEGFTSHDVVARVRRNLRPSVKKVG
                     HAGTLDPFATGLLVVLAGRATKLARFFVDLPKEYECTVRFGVRSDTGDLTGQLAETGA
                     TTTRAAVDAAIPGFLGHIMQSVPMTSAVKVDGERLYKKAHRGEVVETPVKEVEIEAID
                     VLEFDEAAQTMRCRIACSKGTYVRQLAIDLGEAVGAGAHLEQLSRTAVGDLRLDEAQT
                     LAEFEAAIGEREAGDDHIPGLVPPAVALQFMPGIELSAAQVTAVRNGARLPGGPQEPV
                     RLTFRGELIAIYGPAEEGNALKLLVKL"
     gene            complement(11238..12209)
                     /locus_tag="VFZ86_02880"
     CDS             complement(11238..12209)
                     /locus_tag="VFZ86_02880"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEX5641265.1"
                     /translation="MKPLAADLDAVCRRMRGESRVALAVHEKPDSDALGAAAGMLDLF
                     AQLGAEAHLYVSEDELLPLGDVLLPAERVRRGAPPSGAALYALDSGNPDRLALPDWGE
                     VAVNIDHHHDNPRYGELAFVRGEASSTSELVCDIARALELRPSPAAAAALYAGISFDT
                     GHFHHDSTSPSTFRTAAWLVELGVDVTGMYALLYERRSEASLRLWARAVAGARAVAGG
                     RALLAAVTRADYAATGAGPEETEGVVDSLRGVGGVEVAALVKEQTGGARVRVSLRSET
                     IDVSAVAALQGGGGHKLAAGFSSDDGPEEVIAWLSSELARRLSTASS"
     gene            complement(12206..12670)
                     /gene="rbfA"
                     /locus_tag="VFZ86_02885"
     CDS             complement(12206..12670)
                     /gene="rbfA"
                     /locus_tag="VFZ86_02885"
                     /inference="COORDINATES: protein motif:HMM:NF014130.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEX5641266.1"
                     /translation="MGLRLLKVNESIKETLSSVITSAGLKDPRIGFVTVTGVETSRDL
                     RHAKVFVSVLGGQTERELTMKALEKSRGFLQAKINASLHMRHTPQLQFFYDETLDNAL
                     RIERALKREAEVLGEEPHEIPVPGAEPEAEGDEGAADAADGGETPPAGEDDA"
     gene            complement(12652..12975)
                     /locus_tag="VFZ86_02890"
     CDS             complement(12652..12975)
                     /locus_tag="VFZ86_02890"
                     /inference="COORDINATES: protein motif:HMM:NF016351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HEX5641267.1"
                     /translation="MRPDACLGVYLAELHFPEARTLKEKRAPLASLRDVLQSRFRASF
                     SEVGQQDAWQRARVLVVIAASSLQQTRERLDDIDRYLHGRDFEVARVLLKTSDTVDTV
                     WDFDS"
     gene            complement(12965..15043)
                     /gene="infB"
                     /locus_tag="VFZ86_02895"
     CDS             complement(12965..15043)
                     /gene="infB"
                     /locus_tag="VFZ86_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012009993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEX5641268.1"
                     /translation="MAKRKVSDIATQRKTSVDDVLAHLKKAGIEVVDGEVSVDDEAIE
                     KAYAAPAGGAVRRRSGGGPAGKKRRVILDAGASRRGGGPGDRRRGRGRGRGRDREEQQ
                     TAPVVPTGLVKVPSGSTVKDISQLLGITTPDIIKALMGYGEMVTITQTLSDEAIEVLA
                     TDFGREVEITHAADEEAELEKEAVDDPKDLKPRPSVITVMGHVDHGKTSLLDAIRDTA
                     VAAGEAGGITQHIGAYQVRHSDQLITFIDTPGHAAFTAMRARGAKVTDIAAIVVAADD
                     GVMPQTVEAIAHAKAAGVPIVIVINKIDKPDANIERIKQQLAEHGIVPEEWGGDNVFV
                     EASAKKRIGLENFLDMLLLVAEVGDLKANPEAPASGFIIESRVDPGRGVVATMLINRG
                     TIHVGDAIVAGEASGKVRAMRDFRGEPLKSGGPSTPVEIIGFDDLPVAGEFCRVVKDE
                     RAARALGQKRANRLKTEALAKQHALTLDDVFARIAAGKVLDLNLVVKADVQGSLEALS
                     DALLKIQHPEVKVRIIHSGVGGVNESDVMLASASAAIIIGFNVRPSPPATALAETEGV
                     DIRTYRVIYKVTEDITAALVGMLKPTIEEIVLGQAEVRTTFKASKLGTIAGCMVTHGK
                     IVRNAGVRVLRNDVVVHDGKMTSLKRFAEDAREVLEGFECGILLDGFNDLKEGDVLEA
                     YETREVAREA"
     gene            complement(15049..15321)
                     /locus_tag="VFZ86_02900"
     CDS             complement(15049..15321)
                     /locus_tag="VFZ86_02900"
                     /inference="COORDINATES: protein motif:HMM:NF016204.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEX5641269.1"
                     /translation="MIAVATPERTCVGCRRKRGQDELVRLVAEQGRVVEARPGAPGRS
                     AYLCPEQNCLEAAEKKRAFARAFRGPVTLDPGVRAVFARPQDDERR"
     gene            complement(15318..16631)
                     /gene="nusA"
                     /locus_tag="VFZ86_02905"
     CDS             complement(15318..16631)
                     /gene="nusA"
                     /locus_tag="VFZ86_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEX5641270.1"
                     /translation="MSREIIEALRQIESEKGIAFEALVAALEDALLSAYKKSPDAAEY
                     AAVEIDTETGDMRVYELIFPEHVDTEALKVYEEHGEGQPAGDTHENWRLDLSGVDMSE
                     VERIEVTPDDFGRIAAMTAKQVILQRIREAERDLMYEEYIDRVGELVTGIVQQSDPRH
                     NTLVELGRVEAELPRGEQIETEHYEHGARIKAVIIRVEESAKGPQVKLSRRSEELVRK
                     LFELEVPEIADGLVEIRQVARETGYRGRDGKPVPGRCKIAVISHADGVDPVGACVGPR
                     GSRVRMVVSELRGERIDIIPYNEDAARFVAKSLSPARVREVIVDDEEKTATIIVPDDQ
                     LSLAIGKEGQNARLASKLTGWRIDIKSQQQMAEAENSLEFGEEDGVDGQCMAIMRTGK
                     RCPNQAEPGSRYCSIHADYVPAEPAAPPATEEAAAAPAEAETNEE"
     gene            complement(16624..17088)
                     /gene="rimP"
                     /locus_tag="VFZ86_02910"
     CDS             complement(16624..17088)
                     /gene="rimP"
                     /locus_tag="VFZ86_02910"
                     /inference="COORDINATES: protein motif:HMM:NF036577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEX5641271.1"
                     /translation="MRETREELEATIEAALAERFPDVDLVDVEVRGRPASTLTLFIDR
                     PGGVDLDLCAAVSQSLEELRERYALEVSSPGLDRRLRKPAHFAAALGSEVAVTTARPI
                     EGRRNFRGRLAAADDQSITLTLEGGSSATLPLESLGKAHVVYNHETDGGHRE"
     gene            17291..17575
                     /locus_tag="VFZ86_02915"
     CDS             17291..17575
                     /locus_tag="VFZ86_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641272.1"
                     /translation="MGEYTTFECRQCGYQAERIRWGVSVYDPRQRFMPGYCVECGTIV
                     EVDMTGADIHVDEFRCVVCGSELFFLEKAASYTCPKCKAHDMALHQGEYW"
     gene            complement(17583..18383)
                     /locus_tag="VFZ86_02920"
     CDS             complement(17583..18383)
                     /locus_tag="VFZ86_02920"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEX5641273.1"
                     /translation="MNSRTRDIVIVVAGLAVLLAAALGGYAAVRARAQGSGAGQVLAP
                     VAQPTPAPSASAEADPAPQYQRWTVGKAVGPVTVYTRPSTSAPVKTKLGKVNQNGYPT
                     LVLVDSTKETGGRVWYRVWLAMRPNGSRGWVPEGKLAFYTTSSRITIDLSERTLSVYR
                     RGELIGTYPVAVGKPGLSTPTGSFFVNQKLKPPAPGGAYGVLAIGISAFQPKLPDWPQ
                     GGPVAIHGTNQDELIGKAVSHGCVRMHDKDVLAVSRYVPAGSPVVITK"
     gene            18497..19057
                     /locus_tag="VFZ86_02925"
     CDS             18497..19057
                     /locus_tag="VFZ86_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEX5641274.1"
                     /translation="MIAVIVLIVIVVVIVAVLAGMYNSLVKRRNQVDNSWSQIDVQLK
                     RRHDLIPNLVEAVKDYMSYEQETLTNVTQARAAAIDAGNQGPAAVAEAENMLTGTLKT
                     LFAVAENYPDLKANQNVMQLQEELTSTENKIAFARQFYNDSVLTFNNKVQTFPSNLIA
                     GMFNFTTRQFFEVPEAEKAVPTVDLR"
     gene            19086..20129
                     /locus_tag="VFZ86_02930"
     CDS             19086..20129
                     /locus_tag="VFZ86_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX5641275.1"
                     /translation="MYEQISRNRRASWLLAGTVVAILAMLGFAIGYATIGGTQGGIGL
                     LGVFGVVAIAWSIIGYYTGDKMVLAVSGARRVTHAEEPRLFNVVEEMTIAAGLPQPPA
                     VYVLDEATPNALATGRDPQHASIAVTRGLLDRLDREQLQGVIAHEMAHIRNYDIRFET
                     LVGILVGMIALIADFFLRWSFWGGMSGRRRGSDSGGDQASAIFAIVALVLAIIAPLAA
                     YAVQFAVSRRREYLADASAVELTRNPLGLARALNDIAADPRPLRHANRATAHLYIENP
                     MKKTKDVTGVFDTHPPVRQRIRILLEMAHVGPEALVEAPPRAGATPPGPVTPAPPAAG
                     GPWVPPPGPGTPG"
     gene            complement(20154..20543)
                     /locus_tag="VFZ86_02935"
     CDS             complement(20154..20543)
                     /locus_tag="VFZ86_02935"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5641276.1"
                     /translation="MEPPERRTPDFCAESEAYSLFRRGAGFLEGGHPAQAAMYLSRAL
                     DLEPGRNSIRETLGRAEFALGRYERAAALFREVVADVPDCDYAHYALARSLRALGRGE
                     EARAHLRLARALHPTCATYRRRLDGVD"
     gene            complement(20555..21112)
                     /locus_tag="VFZ86_02940"
     CDS             complement(20555..21112)
                     /locus_tag="VFZ86_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HEX5641277.1"
                     /translation="MTPERALVLAEVEAHVANENLRRHMLATEAIMRALAARLGEDPD
                     AWGLAGLGHDLDAEETEDDFTRHGAVAAETLRGLGLPEDAVHAVLAHNPDTGVAATER
                     VDVALVASDQLSGLITAAALVRPDKDLAGVKVKSVRKRYREGAFARGVDREAIARCEE
                     LGLEPDEFFALGLEAMQGIAPRLGL"
     gene            complement(21109..22302)
                     /gene="coaBC"
                     /locus_tag="VFZ86_02945"
     CDS             complement(21109..22302)
                     /gene="coaBC"
                     /locus_tag="VFZ86_02945"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEX5641278.1"
                     /translation="MHAARTVLVGVTGGIAIYKAVELVRGLVKDGFRPLVVTTEAAQR
                     FVMPLTFAAVSQAPVLDDQSAWQASGGWFQHIEAARTASLMVVAPATANTLAKMAGGM
                     ADNLLTAIYLAFRGPVIVAPTMNWAMNEHAATRENLRVLAERGVEVLPTEEGELACGE
                     EGSGRMASPDAIRLAVRRALAGAATGPLAGKKVVVTSGGTRERIDAVRFIGNRSSGKM
                     GRAVADEAYLRGARVALVTTQPADGAPYDVLRVESAAEMAAAVAEQVRDADVLVMAAA
                     VADFRPKDAEGGKIERGERESLTLELVRTVDVLASSARPGLYRVGFAAEAGPRLDRAR
                     AKKAAKGVDLLVFNDILAEGVGIGSDENEITILTPGGEVHVPRTTKTACARAIADQIE
                     GGLTR"
     gene            complement(22331..22570)
                     /gene="rpoZ"
                     /locus_tag="VFZ86_02950"
     CDS             complement(22331..22570)
                     /gene="rpoZ"
                     /locus_tag="VFZ86_02950"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEX5641279.1"
                     /translation="MDKPRIDELLPHADGSRYELVMIAAKRARQINNYYHSLGENTLG
                     LEELTPPLISTRSTNLLTIAFEEIVDNKIDHETSE"
     gene            complement(22571..23131)
                     /gene="gmk"
                     /locus_tag="VFZ86_02955"
     CDS             complement(22571..23131)
                     /gene="gmk"
                     /locus_tag="VFZ86_02955"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013251849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEX5641280.1"
                     /translation="MPSVFVISGPSGAGKGTVIALVRTRLERVVTSVSATTRAARPGE
                     SDGREYHFMDEAAFRAAVTAGDFLEWVEYSGNLYGTLRSEVEAKLAAGDDVILEIELI
                     GARAVRAAMPEAITVFIAPPSMAELAERLRGRGTESAAAIAKRLHRAEAEVAAAEEFD
                     HVVVNDDAGRAAGEVAAIIEAQRKED"
     gene            complement(23135..23467)
                     /gene="mihF"
                     /locus_tag="VFZ86_02960"
     CDS             complement(23135..23467)
                     /gene="mihF"
                     /locus_tag="VFZ86_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor, actinobacterial type"
                     /protein_id="HEX5641281.1"
                     /translation="MSPSSKQFNTAPERSLDQRMDALGRANEVRSRRAALKAELKHGS
                     VSINEVLADPPEFLLTAKVADLLMAAPKCGRVKSARIMEQCRISPSKTVGGLSERQRH
                     ELLAYFGG"
     gene            23747..24145
                     /locus_tag="VFZ86_02965"
     CDS             23747..24145
                     /locus_tag="VFZ86_02965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX5641282.1"
                     /translation="MSPECACRSDQLSGSRALVTASGELDVNTCPQFQKAIAAAARRD
                     AEHLLVDLSDVTFMDSTALGVLAAEQRRRSEPLHLIVKERQLQRLLEVTGFVFVFAVH
                     SSLEEALLRTGRLQAERARPVDLAPARDRP"
     gene            complement(24170..25132)
                     /locus_tag="VFZ86_02970"
     CDS             complement(24170..25132)
                     /locus_tag="VFZ86_02970"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF005574.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEX5641283.1"
                     /translation="MTAASPELSVRLGPIELEHPLVDASGTFDLLEYARRYDGDYFAD
                     FPFAAYVPKTVTADARTGNPPPRVTETAAGMINAIGLENPGIHAWIGGLAEWAALRQP
                     VVVSVGGNDPAQYAAVIGALEAHLAAAPPAAAPRIEGYELNISCPNVSSGLQIGADPA
                     ATEEVVGACRAATGRFLLAKLTPNVRDVTEIGAAALGAGADGLSLVNTFKALVLDRHT
                     LRPFLGNRTGGLCGPAIKPIALRMVAEVAEAFPDTPLVGMGGVMTGRDALEFIACGAT
                     AVAVGAANFTAFEAPQRILAELREELREHGFASVAEARGAALRA"
     gene            complement(25129..25920)
                     /locus_tag="VFZ86_02975"
     CDS             complement(25129..25920)
                     /locus_tag="VFZ86_02975"
                     /inference="COORDINATES: protein motif:HMM:NF021903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydroorotate dehydrogenase B
                     electron transfer subunit"
                     /protein_id="HEX5641284.1"
                     /translation="MDGAPAQMHGDHSQWAETTHCRVAGVRHFGAFAAVELIAPRVAR
                     RAAPGQFVMVAVPGGGFLLRRPLSLFAVHGDRISLLVEARGDGSERLTQLDVGDGVDV
                     AGPLGSAFPTEGVTNALLVGGGIGCAPLQYLADELTAAGAHVTAAFGFRDFRAARAAG
                     AFRIDRLWVATEDGSVGKAGTALHLLAALDVAPGTTVYACGPTPMIAAVQQWTFAEGL
                     RGYVSLEAHMACGTGSCHGCVVDTTRGKVRVCSEGPVFRLDEVTP"
     gene            complement(25923..29114)
                     /gene="carB"
                     /locus_tag="VFZ86_02980"
     CDS             complement(25923..29114)
                     /gene="carB"
                     /locus_tag="VFZ86_02980"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010878769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEX5641285.1"
                     /translation="MPRRDDLHKILIIGSGPIVIGQACEFDYSGTQACKVLLDEGYEV
                     VLVNSNPATIMTDPAFATRTYIEPLDLATLTRIIEVERPDALLPTLGGQTALNLAMEL
                     KAAGVLDLWEVEMIGADEKAIHRAEDRLEFRTAMEEIGLRVPRSMLAETMDEAETACA
                     ELGLPLVVRPAYTLGGSGGGIAYDHDSFTRICEQGLALSPISQVLLEESVVGWHEFEL
                     EVMRDRADNVVIICSIENIDPMGVHTGDSVTVAPALTLTDAQYQELRDASKAIIRKIG
                     VDTGGSNCQFAVNPETGEVVVIEMNPRVSRSSALASKATGFPIAKIAARLAVGYTLDE
                     IDNDITRKTPACFEPTLDYVVVKMPRWAFEKFPLADRELTTHMKSVGEAMAIGRTFKE
                     AFQKAVRSRELDAPTDLPGSAPEIRKALAIPTAERYELVLHAFRQKLGVDEVHELTGI
                     SPFFLSELADIVELEREVREAPRLTPELLRRMKRYGFSDKRLGELTGRGEREVRALRL
                     EHDVLPVYKAVDTCAAEFEAETPYFYSSYEEENEAEPGERERVVILGSGPNRIGQGIE
                     FDYCCVHAALTAREQGFEAVMVNCNPETVSTDYDISDRLYFEPLTFEDVAGIVANERP
                     RGVIVQFGGQTPLKIARALAEAGIPILGTPQESIDLAEDRGRFGGLLEQLGVRCPEYG
                     LATTAAEAAEVAARVGYPLLVRPSYVLGGRAMQIVYGPKELEHYMQTAVSVSPEHPVL
                     IDKFLEDAIEVDVDAICDGEDVYIGAVMQHVEEAGVHSGDSACVIPSISLGEGTLEQV
                     RKQTKALALGLGVKGLINVQFAYQSYQLYVLEVNPRGSRTVPFVSKATGVPMAQIATR
                     VILGEKLKAMGLERRVPEYVSVKEAVLPFNRFPGTDTQLGPEMKSTGEVMGIAEAFPA
                     AFGKAVAAAGMPLPTEGDVFLSVCDSDKSAATILAQRLHMLGFAIYATRGTATALTSL
                     GIPVNVVNKVFQGEPNVVNLIESGQIGLVINTPFGRGARADGYEIRTTALRKGIASIT
                     TLAGASAAVSAIEALQRPRVQVHCLQDLHAATD"
     gene            complement(29114..30223)
                     /gene="carA"
                     /locus_tag="VFZ86_02985"
     CDS             complement(29114..30223)
                     /gene="carA"
                     /locus_tag="VFZ86_02985"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEX5641286.1"
                     /translation="MPSPAVIVLEDGATFAGEALAGSGSVGGEIVFNTSMCGYQEIAT
                     DPSYCGQLITYTFPMNGNYGADAERDESGKAHARAIIARELTNYRFNRASRQTWLDWL
                     AEHGVLAVTGVDTRALTRHIREKGALRAVVSTDVADVKHLRKSAQGLPAMGGLDLAKV
                     VTCETAYEAPAPEGAPAPDLHVVAYDYGIKRSMLGYLARRGFRVTVVPAQTSAQKVLK
                     MEPDGVFLSNGPGDPAAVTYAVKAVERLLGKVPVFGICLGHQLLAQALGMKTFKLKFG
                     HRGANHPVKDLRTGVIEITTQNHGFAVKDEQVPGAEITHVNLNDGTVEGVAAPERYAF
                     SVQYHPESTPGPHDSLYLFDRFRDEIDRFKRGGEA"
     gene            complement(30216..31532)
                     /locus_tag="VFZ86_02990"
     CDS             complement(30216..31532)
                     /locus_tag="VFZ86_02990"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEX5641287.1"
                     /translation="MERTWCKPLPAAELLITGGRVVDPEAGVDAVQDVLVKKGKVAEV
                     GKGLKAGKNTRVVDAAGMLVLPGFVDLHAHFRTPGREDEEDLATASAAAAAGGYVAAF
                     GMANTDPVVDNAGLLKGLALQAGADAVVPVGFFAAVTKGLEGQELTEMGELGAAGAVG
                     FSDDGRPLPTAAITRRALQYVKVSKRFVAIHAQDDSLFKGGQMHEGPASARLGLTGIP
                     SLCESLDVSRSLDIAAYEDADLHVCHVSTAASLEAIARAKEAGVRVTAEATPHHLTLN
                     DERVAALDPNLKMNPPLRAESDRKALVEALRSGLVDCVATDHAPHVSEEKDVPYEEAP
                     FGCIGLETAFSVLHAELVASGELDLATLVTRMSSAPARIAGLEAPSLTAGATADLCVV
                     DPAAVWTVTPEALQSRSWNSPWLGRELTGKVRLTVAAGRVAWDDLA"
     gene            complement(31532..32473)
                     /locus_tag="VFZ86_02995"
     CDS             complement(31532..32473)
                     /locus_tag="VFZ86_02995"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005010418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEX5641288.1"
                     /translation="MPKRDLISIEDLSRADVELLLDTAESFLPVLERDIKKVPTLRGR
                     TIINLFWEASTRTSSSFDLAAKRLSADTLALKSSGSAVEKGETLKDTAITLSAYSPDI
                     IVMRHPSAGACQTLSANTEASVINAGDGKHQHPTQCLLDLFSMRQNLGRELDGLRVFI
                     VGDILHSRVARSDIMGFQMLGMDVTLAGPPTMMPRGIEEMGVNVAYDLSDLEEADVIY
                     LLRIQKERIKPGSAYLPSLREYAQLWGISQARLGPKQRVMHPGPINRGVEIASDIADD
                     DENLIAHQVFSGLAVRMAILYRTIVGYSDGAASGEVL"
     gene            complement(32477..33058)
                     /gene="pyrR"
                     /locus_tag="VFZ86_03000"
     CDS             complement(32477..33058)
                     /gene="pyrR"
                     /locus_tag="VFZ86_03000"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014685478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEX5641289.1"
                     /translation="MPQPRPASETVVLTGEQMSRTITRIAHEILEKNPGEEKLAVVGI
                     HTRGAFLGARIHKLLCEVGGREYATGNLDISFYRDDVSARGAVGVIPSTPQPVVKDTD
                     LPFTIDGARIVLVDDVLYTGRTIRAAIDALFDYGRPATVQLAVLVDRGHRELPIRPDY
                     VGKNLPTAARERISVRVEEIDGVDEVVLVRKEA"
     gene            complement(33194..34123)
                     /locus_tag="VFZ86_03005"
     CDS             complement(33194..34123)
                     /locus_tag="VFZ86_03005"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX5641290.1"
                     /translation="MAGRSEARAFRAAAGDAGERLDVALAAHAGVSRAAAQRLIAGGL
                     VAVDGAPARKNHVLRAGEEVVWQAPPPPAVTLAAEPVPFTVVYQDGWLLVVDKPAGVV
                     VHPAPGHEHGTLAQGLVAEGARGGHELRPGIVHRLDKDTSGLLIVARRDEAYRRLVAA
                     MERRDIHRTYLALLSGDLPQARGTIDAPIGRHLRDRKRMSLHTAAGRPAVTHFEVLGR
                     APGYTLVRVRLETGRTHQIRVHFQALGYPVAGDVQYGRAPRPDGLGRQFLHAARLAFP
                     HPEDGREIRCCSPLPPDLAAFLEALGLPGQPEA"
     gene            complement(34107..34556)
                     /gene="lspA"
                     /locus_tag="VFZ86_03010"
     CDS             complement(34107..34556)
                     /gene="lspA"
                     /locus_tag="VFZ86_03010"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEX5641291.1"
                     /translation="MARSNRGYVLALILAAAVVVADQATKWVVRAQAEHLPRQLAGGL
                     RIDLTYNSGISFSRFAGAGDVVVVLVAAVAAGVAVALVLSPPRYRPALGIILGGAAGN
                     LIDRLRFDGAVVDFIGFYGWPSFNVADAAIVVGTVLLALQVVVGRQE"
     gene            complement(34543..34707)
                     /locus_tag="VFZ86_03015"
     CDS             complement(34543..34707)
                     /locus_tag="VFZ86_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641292.1"
                     /translation="MAKLAYCKQCKATYPLNIVVNDGGRSGKAKEPTCPMGHVDIKQL
                     DAVPKPRGKK"
     gene            complement(34727..34984)
                     /locus_tag="VFZ86_03020"
     CDS             complement(34727..34984)
                     /locus_tag="VFZ86_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HEX5641293.1"
                     /translation="MSLSVWVTPGARRSELAGVADGRLRVRLAAPASEGRANDELIRC
                     LSRELGVRRQDVTVVAGGRSRRKLLRLTGVTLDEARRRLAL"
     gene            complement(35016..35981)
                     /locus_tag="VFZ86_03025"
     CDS             complement(35016..35981)
                     /locus_tag="VFZ86_03025"
                     /inference="COORDINATES: protein motif:HMM:NF016959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEX5641294.1"
                     /translation="MKLTPLDIRHKEFKRGMRGYADVEVDEFLDQIADEYERLFKENI
                     DLQDRVESLEEKVAGYRRIEETLQKTLVNAQASAEEQKQNANKQAQLVLQDAELKARQ
                     LVNEAYSERQAIEQSMAKLRSAEEDFRFKFRQLLDGYLRQLQDAPEVAEAPPASDFSR
                     HTAAIKEAIAREAAPARPEPTPAPSAVTPEPAEGVADAPVPAEEPVAAPDPPAATEDE
                     HEPDPEPEPERRYVIPGEPAVVPQRGRSGVQAGVHTEPAPSDGEGRPDRPREDDPEVT
                     AAHPAPEGAPDDEGRQKRRSRILFGESDDLLADVDTGVNEDEFKW"
     gene            complement(36049..36852)
                     /locus_tag="VFZ86_03030"
     CDS             complement(36049..36852)
                     /locus_tag="VFZ86_03030"
                     /inference="COORDINATES: protein motif:HMM:NF026098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase dimerization
                     domain-containing protein"
                     /protein_id="HEX5641295.1"
                     /translation="MRPRDAVRVGCVGAGHIARALGRGWARPEVSQPPRLTYLDVSAE
                     AAAHAAADTGASLAASLPDLIATSDVVVVAVRPQHVAGVLAEAGPLLGDRPLVSVAAG
                     VPLAELRAALPGDARVARVMPNVAAALGLGVFLFVPGTLAERTAEVGELFALAGDVIE
                     LDERDFDVATAVAGCMPGMLALLVRDFGRAAERRGLAPDVARRLAIAGVHGAAAVISH
                     DGDPDAVIASAATPGGMTAAAIAALEERDVAEAVALAVQAAAERAKELA"
     gene            complement(36892..37428)
                     /gene="sepF"
                     /locus_tag="VFZ86_03035"
     CDS             complement(36892..37428)
                     /gene="sepF"
                     /locus_tag="VFZ86_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEX5641296.1"
                     /translation="MGLGDWWHKTAVYFGIADDEDDYDEDETLAPHEELERSYQERPN
                     VRKLNSAKGRNDYDDIFADEPRRRAPASSQPAVRALEPARPARVEVHLVIPKNFNDAQ
                     QIADKFKVDVPVIINLQGSDTELAKRLIDFCSGLTYALDGGMQRVGEKIFLLTPRNVE
                     VSAEERARLIEKGFFNQF"
     gene            complement(37448..38140)
                     /locus_tag="VFZ86_03040"
     CDS             complement(37448..38140)
                     /locus_tag="VFZ86_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEX5641297.1"
                     /translation="MVRQFVPDVETVRERYAEVESRVAEAAGAAGRDPAEVRVLVATK
                     YVAAEHLGALRDAGIRLIGENRAQDLVAKHELYGDAFDYHFIGHLQSRKTRLVLPIVS
                     LIHSVGSTSVVQEIEARAANATDVLLEVNIGGEQSKSGVALGEVDAFLETAAASPKVR
                     FTGLMCMPPLFDDAEEARPFFVRARELAQRLSARWSGTYTFEQLSMGTSADYEVAVQE
                     GATIVRVGSVLF"
     gene            complement(38176..38964)
                     /locus_tag="VFZ86_03045"
     CDS             complement(38176..38964)
                     /locus_tag="VFZ86_03045"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HEX5641298.1"
                     /translation="MTSAGFAWRERDGVSWLAWEAGGVTAAFPARTGGVSAPPFDSLN
                     LGLSTEDRAEDVLENRRRLCAAVGLSPGRLVVPGQVHGTDLRWVGEDEAGRGAFDVET
                     VIGEHDGLLTRAKGLGLAISYADCVPVVIVAEGEEGTAYATVHAGWRGMLAGIVGEAA
                     AELARSGRRPVAAAVGPSIGPCCFTVDESLRRRFEARFPGSAGAATVDLWRCARLELE
                     AAGVPARSVAVAGLCTASDARFFSHRRDRGVTGRHLAVAWRQEA"
     gene            complement(38961..39614)
                     /locus_tag="VFZ86_03050"
     CDS             complement(38961..39614)
                     /locus_tag="VFZ86_03050"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX5641299.1"
                     /translation="MNDSGLFLQFLVVSPLLLVSLVLHELAHGWVAYRLGDPTAKAHG
                     RLTLNPIRHLDLWGTVMLAVTFLGSGGSFFFGWAKPVPVDPRFFKDGQRGMAWVGAAG
                     PLTNFALVLVATGLIWLTYTWSLFLLEMFWMLFVLNVILMTLNLIPIPPLDGSRVLGG
                     FLPRATYRKWAELDRFGNFVFIGLFLVMIAVPGFFEATFGTVLDVFRNLLLPGGWFW"
     gene            complement(39611..42232)
                     /locus_tag="VFZ86_03055"
     CDS             complement(39611..42232)
                     /locus_tag="VFZ86_03055"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HEX5641300.1"
                     /translation="MRTPELIATHSNTDFDAFAAMVAAHKLYPEAQISLGGAVNRNVR
                     EFQALYADAIPVVDPADVDRDSVRRLILVDTVHANRLGDLGDLCGRAGVQVVAFDHHA
                     HPDDLPGYLDPDALVSSGDGSLVTLLVRIIAERGIPVTPFEATVFALGIHEDTGSLTF
                     ATTTTRDAEALAYCMRAGADTELLEKWLTNALTPAQRLTLAAALQTAHELPIAEASVL
                     LSSLRQDLYVEGVSVVAHRVMDLTGCDAYFLLVEMEGRVFVTARSRGGRVDVAEALRA
                     VGGGGHTAAASAVVKDRSLDEVAAAVAAAAPGAVAPTRVALDALEPGPVVLADVAAVD
                     DAALLCRRDGLGGVGVVRDGLLAGRVALADLERASAHGLGHAPVKAVMTTSVPLVGAQ
                     TPLERVALRLAQDEVGWLPVVAGESARSEDEGVALGDLLGVVTRRSVAGAPPREPAAP
                     VTANLAGPLGDLGLDELFRHVQAVAAGVRGCYLVGGAVRDLLLGEPGFDVDLAVEGDG
                     IAFATELAARLKGHVRPHQKFGTAVVVADDGTDRLRVDVASTRAESYAYPGALPKVEH
                     AGIRSDLARRDFTINAMAVSLKPETFGDLFDYFGGREDLLAGRIVVLHNLSFIEDPTR
                     ILRAIRYESRYELRMDGHTLNLARACCAMDLVGDLSSARLRDELVSLLDEQKVDFALR
                     RAEELGVWPAIHGRLRVDGATRRLVRRADELRAAHALERETPRWRLRLVWLLRDLDPE
                     EIAAWAVRMRVRAQDASVLERALVVAGRLLDRIARQPGEAELYDLAAGEPLEAVLAAM
                     TLDESGIAADRLAHFLDVSRHVRLDIGGADLLALGFSSGPRLGEVLRSVLHLKLNGVV
                     GGREEELTAAARMRESA"
     gene            complement(42268..44430)
                     /gene="recG"
                     /locus_tag="VFZ86_03060"
     CDS             complement(42268..44430)
                     /gene="recG"
                     /locus_tag="VFZ86_03060"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEX5641301.1"
                     /translation="MTGQPLPLRFGGGRYDERPPLTRLSFDPSSLSLPLAGLDQVTPA
                     VARRLAGFGLHTVGDLLVHFPRRYEDFRDRKAIRDLRVGEEATIRGEVVRVRAERTAR
                     RRVDIVRVLLRDDTGAVDAVWFNQKYLTRILQEGMRLSLRGTFRPQGGRQSFVVKGHE
                     ILDEEVGEGVHTEGVVPVYPASEQVSAKLLRALVHAVAPVMRRLPDPLPAELLVREGL
                     PGRADAVTAVHLPRSLAEAGAARERLVLEELLLMQVGLLRHKAEQQQRVHAPALPEPG
                     ELSRTFLEGLPFELTAHQLGALDELEGDLRRDVPMRRLLQGDVGSGKTVVALYCLLRA
                     AEAGLQGVLLAPTETLAAQHAETAARLAGALAPAELVTASLPAAERRAALARLAAGEA
                     RIAIGTHALLQEDVRFAGLALLVVDEQHRFGVEQRDALARRAERDGVAPHVLHMTATP
                     IPRTLALTVFGDLDVTTIAGVPSGRQPVVTRLVDETQRETGYEFVRKQIARGRQVYVV
                     CPAIDESESISAATAVAEAERLQAGPFRDARVAVAHGQLRSADRDEVMRAFKTGDLDV
                     LVATSLIEVGIDVPNATVMIVEGAERFGLAQLHQLRGRVGRGSEKSYCLLFAEAETGS
                     SQQRLEALLETNDGFELADRDLEIRGEGQVLGARQAGVPDLKLARLARDRSALLRARE
                     LAAGILAADPGLASPVNAPLRDAMEAAFGHELGWLLKA"
     gene            complement(44427..45287)
                     /locus_tag="VFZ86_03065"
     CDS             complement(44427..45287)
                     /locus_tag="VFZ86_03065"
                     /inference="COORDINATES: protein motif:HMM:NF014679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEX5641302.1"
                     /translation="MTDLLTLDNTAIVLDSTCDAPSGWLQQPGLYMVPLKVHFGDQMY
                     RDGVDMTSQEFFARLEQAEVLPTTSQPTAGEFVAVYEEARRQYEHVFSLHLSGEMSGT
                     VRAAEQAAEQVDHVYVYDLRTVTTALSMCAERLRVRLAAGCTLDDAHAYVRHFRDHSE
                     LLVHAATLEYLRRGGRIGRAASLVGGVLDIKPLIAAVDGTLTGYAKVRGQKKALAAMQ
                     RFVEERSRPEDELYFCLIDAVNDEEPPLIREMIERVRPNAHFTFLGHVGAVVGTHIGP
                     GTAAFAMIVE"
     gene            complement(45420..47075)
                     /locus_tag="VFZ86_03070"
     CDS             complement(45420..47075)
                     /locus_tag="VFZ86_03070"
                     /inference="COORDINATES: protein motif:HMM:TIGR03599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEX5641303.1"
                     /translation="MIVRTAAIGVIQSALAAVEAERQFINDLNVYPVPDGDTGTNMAL
                     TVRAVVEELEQSDVRDIPSVAAAVTKGSLMGARGNSGVILSQIVRGICEVWGQANSLD
                     TAVFKRAVVEGQHAAYRAVKTPVEGTMLTVIREMATAAQGVPDSLGLEHLLAAVEEAG
                     RIAVENTTAQLPALQKAGVVDAGGYGLLVLFRGLAAGIEDLMRGGKAIAGVRVAARAA
                     GEAFIGAVRPARPHAVTVEEPSELSEFRYCTSFLVTGESIDREAFEAFLLPLGDSALV
                     VGDEGTVKVHVHVNDPGAVLSEALKHGAIGDVEINDMHAQTRERDERLATPAPEVGEG
                     GAVVVAVVAGAGNRQLFRELGCHAIVDGGQSMNPSAAQLLEAVEALGVQEVVILPNNG
                     NVILTAEQAAGMSSLHIAVVPSRSIPSGLAAMIAFDPAQDAVGNARLMQDAIADVRYA
                     EVTHAVRDSELDGLQVKKGQTMGIVDGTLVAAADDVESAFGGVLEEFARGGAEYVTVL
                     TALNGSGTTREQLEEVAARVAPDAEISFHEGGQPLYPILASAE"
     gene            complement(47072..47437)
                     /locus_tag="VFZ86_03075"
     CDS             complement(47072..47437)
                     /locus_tag="VFZ86_03075"
                     /inference="COORDINATES: protein motif:HMM:NF015721.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEX5641304.1"
                     /translation="MSQSDLRIPFTAGGIVGGVTVAHQVIAKLAGVAARATYGVVGMQ
                     DPTVKKIARFFRGSVTEGVELAVGEGSVDIGLHVVMERGVNIAQVTANLREQVRYQVE
                     HVGGVPVGEINVRVEDLQD"
     gene            complement(47447..47650)
                     /locus_tag="VFZ86_03080"
     CDS             complement(47447..47650)
                     /locus_tag="VFZ86_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HEX5641305.1"
                     /translation="MRFTKDMSIFEALSTDPAAREVFESHGMACCLCIGAQSESIEAG
                     AILHQVDPETVVAELNQLITAGE"
     gene            47732..47923
                     /gene="rpmB"
                     /locus_tag="VFZ86_03085"
     CDS             47732..47923
                     /gene="rpmB"
                     /locus_tag="VFZ86_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEX5641306.1"
                     /translation="MSKICVTCGKAPAVGNNVSHSHRHTKRRFEPNLQRVRILVDGHP
                     QNAYVCTRCLKSGRVSKAV"
     gene            complement(48211..48675)
                     /gene="ribE"
                     /locus_tag="VFZ86_03090"
     CDS             complement(48211..48675)
                     /gene="ribE"
                     /locus_tag="VFZ86_03090"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEX5641307.1"
                     /translation="MTTYEGKLNGKGMKFGLVVGRFNELISGRLYEGALDCLRRHDVD
                     DADVDAAWVPGAFEMPLVAKRLAESGKYDAVICLGAVIRGGTPHFDYVAGEAAKGIAK
                     ISLDTGVPVIFGVLTTDTIEQAVERAGTKAGNKGWSAASTALEMANLAKTLP"
     gene            complement(48705..49889)
                     /locus_tag="VFZ86_03095"
     CDS             complement(48705..49889)
                     /locus_tag="VFZ86_03095"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEX5641308.1"
                     /translation="MVIVCDDEDRENEGDLTMAAQFATAENINFMAKEGRGLICLALT
                     PQRCGELDLPPMTSHNEARLQTAFTVSVEAREGVTTGISAADRAHTIQVAIDPESRPG
                     DLVQPGHVFPLMARPGGVLERSGQTEAAVDLARLAHLNPSGVICEIMNDDGTMARVPD
                     LIPYARRHGLKIITVADLIKYRRRNEHLVRRAATVSMPTRYGDFKAIGYESVLDGSHH
                     VAIVKGDVAGKKDVLVRVHSECLTGDTFGSLRCDCGEQLAEAMRRIEAEGLGVVLYMR
                     QEGRGIGLINKLKAYALQEQGYDTVEANVELGFAPDLRDYGTGAQILADLGLSTIRIM
                     TNNPKKIVALEGYGLRVTGREQIEFAPQEDNRCYLRTKRDKMGHMLEHEDLFGQDEGL
                     EG"
     gene            complement(49997..50635)
                     /locus_tag="VFZ86_03100"
     CDS             complement(49997..50635)
                     /locus_tag="VFZ86_03100"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEX5641309.1"
                     /translation="MFTGIVEEIGTLRGIRPGADSVVLDIAATTVTDDAAVGDSILTD
                     GVCLTVTSLRAGGFTADAMPETVRRTTLAQRRPGDRLNLERAMTLRSRLGGHLVSGHV
                     DGVGAVTAVTPEDNALVVELEAPEAVTRVTVDQGSIAVDGVSLTVVAVAGDRVRVSLI
                     PHTAAVTTLGRLTTGSRVNLEADLIAKYVHAFVAGRKPGAGLTWEKLAEAGF"
     gene            complement(50637..>51395)
                     /locus_tag="VFZ86_03105"
     CDS             complement(50637..>51395)
                     /locus_tag="VFZ86_03105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RibD family protein"
                     /protein_id="HEX5641310.1"
                     /translation="GLARLREGGVEVDLAEDRWGARAREQNAPFHKYHATGLPLLTYK
                     AAVTLDGKVAAAGGDARWISCLDSRRAVHELRARVDAVMVGVGTVRRDDPELTVRLSE
                     GRDPVRVVITRDGELPPGARLLASARRTRTVVVADEATEAARRLLDARGAELLEVGDG
                     GLRAALEGLAALGLLDVLFEGGPTLAGGLLAAGLIDRMLLFVAPLIVGRGAPDLFAAP
                     AVESVDGAWRLQDVTWRPLCDDLMLSGTVVRREG"
BASE COUNT         6933 a        18160 c        18547 g         7755 t
ORIGIN      
        1 ctggtcgagg atgatgcggg agtcggtctg cgaggccacg gcgaaggcga tgcgcgaggg
       61 gatgttggtc ttgatcatgc cggtgaccac gtcgaccgac gggcgctgcg tggccaccac
      121 gaggtggatg ccgcagctgc gcccgagctg cccgaggcgg atcacggaat cctcgacctc
      181 cgagggggcg accatcatga ggtcggccag ctcgtcgatg atcaccatcg tgtacggcat
      241 ggggtcctca cccgcgcgca ccagcttctt gttcagcgcg cggatgtcct ggcagccgtt
      301 gcgcgagagc acgtcgtagc gccggtccat ctcgcggcag atgttgtcga gcacgtaggt
      361 ggcctgcttc atgttggtga ccaccggcgc cagcaggtgc ggcacgccgt tgaagttgct
      421 cagctcgacc ttcttggggt cgatcatgat catgcgcacc tcgtcgggcg tggcgcgcag
      481 caggatcgag gtgatgagcg cgttcaggca gccgctcttg cccgagcccg tggtcccggc
      541 gatgagcagg tgcaccatgc gcaccaggtc ggccagcacc gcgctgccgg tgacgtcctt
      601 gcccagccag aaggccatgg gcgaggcgtg ctgcgggaac aggccgtgga tgtcgcccag
      661 cgagacgtag ttcgcctgct ggttcggcac ctcgacgccg acggccgtct tgcccgggat
      721 cggcgccagg acgcgcacgt cggtagccgc cagcgcgtac ttgaggtcgt ccttgagccc
      781 ggccaccttg ctcaccttga tgccgggctc cagctgcagc tcgtagcggg tgacgcgcgg
      841 tcccgagacc gtgccgatca cctgggcggg gatgttgaag ctgcccagcg tcgccaccag
      901 ccgctcggag acgcgggcga cggcgtccgg ggactcaccg tgcccctgcg ccagacggcg
      961 caggagggcg gggtcgggga gcgtccactg acggcgctcc gcctccgcga aggccagcgc
     1021 cacgtcggcc tctccggccg cacccttctc cgcctgcgcg gaggcggccg cggcgagcga
     1081 gagctgcacg ccgtcctccg ccgccacgac cggctccggc tcgtgctcgc cgggcagcgg
     1141 cgtgggctcg ccgaagatct ccggcgcctc cgcggcgccg tcgatgaggt gcggccccgc
     1201 cgcggggacg gggcgcgaga agtcgagctc cggagacacc gccggccgga cggacgtgcg
     1261 catgaggtcg ccggcgtcga tggtctgcgc ctcgtcgagc tccaccacgc gcgtgtcgga
     1321 cgcctccttg cggcgcgcgc ccagggcctc ggcctggctg cgcgccgccc tcccggcctt
     1381 ggcgacgccc tgcctggagt gcgtcgccca ctgccgcagg gacgagccgg tgaccagcag
     1441 cacgccggcc aggatgaggg cgatcacgag gacgtcgacg cccaggcgcc cgatgaacgg
     1501 gctgaggaca gcccactgga cctcgccgag gacgccgccg tgggcgctca tgtaggcgtc
     1561 gcggaagagc gcgtccccgc gctcgccgcc gaagatgccg aaggcgtcgg ccgcggcggc
     1621 gagcgccacc ccggtgacga tcagggcgac gccccagctg agcccacgcc gggggcggct
     1681 ctcctccttg accacgagaa ggtaggcgac gaacccgagg aggaacggca gcgcgaaggc
     1741 gaggatgccg accagccagc gggcggcgtc gcccagccag cggccgagcg agcccccgtc
     1801 ccatcccagg tagagcacga aggcgaggaa cacggcgacg gcgaggcagg cgatggcgcc
     1861 cagctcccgg cggtgccggg aagccggggg cttggtcttg gctcgagagg ccgccgcggc
     1921 gccgcgcggc gccgtccgcg ccgcggcggc cgcgcgccgc cgtgcctgtt tcgggcgtgc
     1981 agtcgccatg gggctgtgtt cgacgcgcgg cggggaaacc ctagcttgcc gcccgcccct
     2041 ccccgccgtc agacctcgac gacgatgggc atgatgagcg gccggctctt ggtgcgccgg
     2101 aagacgagct cggacaggcg ctggctgatg tcctcgcgga tgaggtgctg gccggtgacg
     2161 tgttcagcgc cgagctcgtc caggagcgtg tccagggccg ccccacactc gtcgaggacg
     2221 tcctggagac gcgcgtcgtc gtggaggaac ccccgcgcca cgagctcggg cggggcgacc
     2281 gagtcgccgg tctggccgtc caccgtgacc acggcgatga ggatgccgtc gccggccagc
     2341 tgctggcggt cgcgcagcac gagaccttcg ggatcgccga tctcggagcc gtcgacgaac
     2401 accatgcccg tcgcgacctt gtcgacgatc tcgccgttgt cggcttccag ctcgagcacg
     2461 tcgccgttct cgaggatgaa gatgtcctcg tcgggcatgc ccgactggcg ggccagctcg
     2521 gcgtggaggt gctgcatgcg gtactcgccg tggacgggga cgaggaaccg gggtcgcgtc
     2581 aggttcagca tcatcttgag gtcttcctgg gcggcgtggc cgctcacgtg gatgccgctg
     2641 cgctcgccgt agaccacggt ggcgccgacc ttgagcaggc ggtcgatcgt ggccgtcacg
     2701 ctgcgctcgt tcccggggat ggccttggcg gagatgacga cggtgtctcc ttcgtgcagc
     2761 tccacctgcg ggtggtcgtt gaacgccatg cgcgtgagcg ccgagagcgg ctcgccctgg
     2821 ctgccggtcg agaggatcag gacctcctcg ggccggtact cgtcgatctg cgtgagcttg
     2881 atcatcgcgc cctcgggcac cttgaggtag ccgaggttgc gggcgatgtt tgcgttcttg
     2941 accatcgaac gcccggagag ggcgaacttg cggccgtgca ggtgggcgat ggtgatcgcc
     3001 tgctggatgc ggtggatgtg ggacgcgaag ctggccacga tcacgcggcc ctcggcctcg
     3061 gcgaagatct ggtggaaggc gttgctgacg gtgctctccg aggcggtcga tcccggggcc
     3121 tcggcgttgg tcgagtcgcc gagcaacgcc agcactcccc gttcaccgag ccgggcgaag
     3181 gcgtccacgt cggtgacgcg gccgtcgatc ggcgtgtggt cgaagcggta gtcgccggtg
     3241 agcaccaccg cgccgaggtc ggtgccgagc acgatcgaga gcgagtcggg gatgctgtgg
     3301 ctggtggcga tgaaggagag gtcgaacggc ccaagctcca ggcgcgactt gtcgctgacc
     3361 tcggtcagct cgacgctctt gagcaggccg tgttcctcga gcttggactt gatgagcgcg
     3421 agcgtgaagc ggctgccgta gaccggccgg ttcaaccgcc ggagcacgaa cggcagcgcc
     3481 ccctggtggt cctcgtggcc gtgcgtgatc acgaaggcga gcacgtcggc ctcgcgctcg
     3541 accagccagc ggatgtcggg gagcacgagg tcgatgccga gcatgtcctc gctggggaac
     3601 tcgacgccgc agtcgagcac cacgatcttg ccgcggtact cgaccgcggt catgttcttg
     3661 ccgatctcgc cgaggccccc aagggggacg atacgtacgg cgtcttcact catggtgatg
     3721 ccttctctct gtgttcgtgt cgcggggtgg ccgcggggct caggcgtcgg tgagaccgag
     3781 ccgccggagc tctgcggcca ccacggcttc ttcttcgggt gtggcgggca cgagcgggag
     3841 gcgcaagcct ccgacctcgt gcccgagcag gttgagcgcc gccttgaccg ggatggggtt
     3901 ggcggtgatg aacagcgcct tgtagagctc gtgcagggcg gcgtcctcgg ccgcggcggc
     3961 ctcgaggtcg ccctcccggc agagccgggc gacctcggcc atccgcggcc cggccacgtg
     4021 gctggccacg cagatgccgc cggcgccgcc gcgcgccatg acgtcgtaga gcatgtcgtc
     4081 gttgccggcg tacaccgcca ggtcgctgag ctccatgagc cggtcggtct ccgccaggtc
     4141 ggggttcgcc tgcttgacgg cggcgatgtt gtcgacctcg ccgagctcgg ccagcgcctc
     4201 cggcgccacg ttgatgacgc agcgcccggg gatgttgtag acgatcaccg gcaggctcgt
     4261 cgccgcggcg acggcgcgga agtgcgcgat cacgccgcgc tccggcggct tgttgtagta
     4321 ggggaccacc gccagcacgg cgtccacgcc gcaggcctcg gcggcgcgcg tgagctccgc
     4381 gctgtgccgc gtgtcgttgg agccggtgcc ggcgacgacg ggcgcgcgcc cgtccacggc
     4441 ggcgacgacg gtgcggaaca tcgccagctt ctcccggtcg tcgagcgtcg gcgactcgcc
     4501 ggtggtgccg gcgaccacca gcccgtccga gccgtgggcg agcaggaact cggcgagcgc
     4561 ggccagcttg tcgtggtcga ccgtcccggc ggcgtcgaac ggcgtgacca tggcggtcag
     4621 gatggtcccg agacggtggt ccttcacgcg tcctccttcg gggcccgccc gggcgcgtcc
     4681 ggggagtcca gcaggtgctc gagcccgatg acggtgccgc gcaccgtccc cacgcgccgc
     4741 agcgccagca gcaccccggc catgaacgag cgccgggaga gagagtcgtg gcgcagggtg
     4801 aggagctcgt cctcgccgcc gaacacgacc tcctggtgcg ccaccgcgcc gggcaggcgc
     4861 acgctgtgga tggtcacgcg gccgtcggca acgagcccgc gcgaggccgg cccggcgccg
     4921 ggcggggccg cgagggcgga ccccggcgcc tccgccatga ggcgggccgt gcgcagggac
     4981 gtgcccgacg gcgcgtccgc tttggctttg tcgtgtgtct cgatgatctc ggcgtgctcg
     5041 tagtgccgcg ccgcctcggc ggcgaacttc atcagcagca ccgctccgag ggcgaagttg
     5101 ggcacgatga cgagcccggc gccggacgcc tccgccctgg ccgagaggcc ggcgacctgg
     5161 tcgtcgtcca gcccggtggc cccgacgacg gtgggcaccc cggcctcgag cagggcggcg
     5221 ctgttctcga agacggcgcc ggggacgctg aactcgagcg ccgccgccgg cgtcgtgagg
     5281 gccagggccg cctcgacgtc ctcgaaccac ggcgtctctc ccccgcggcc cttcttcggg
     5341 tccacgagcg cgaccaggcg caggtcgtcc tgcgcggcga tggtggccgc gctcagcgcg
     5401 cccatcttgc cggccgcccc ggtcaccagc acgtcgatca tgacgtccct tcttccggcc
     5461 tgcacgagcg gggcgctaca gacagaccgc tcatcgttcc tcctggacga acccctgggt
     5521 gacggcccgg aacggctccg gctgaggccc gatgcaggcc gtcgaccacg tctccgggtc
     5581 atagtaccgc cccacggcgg cgacggtggc gtccctgtcg accgcgtcga tgcgcgcgag
     5641 cacctcgtcc acggtgagga cgggctgctc cagaaggacg gcccgtccca gcgtctgcat
     5701 gcgcgctccc gggctctcca tgctgagcac cagctgcccc ttgaggtggt tcttggcccg
     5761 gctgatggcg gcgtcggtca tctgcgcctc gaggccggcg agggtctcga ggatgacctg
     5821 catggcctcc tccaccgcct cctggcggga gccgaagtag accgcggcga gcccggcgtc
     5881 cgcgtagagc gacgtgtacg agtagacgga gtaggcgagg ccacgcttct cgcgcacctc
     5941 ctggaagagg tgcgagctcc aggagccgcc gaggatggtg tcgaccacga aggcggcgta
     6001 gcggtccggg tcgccgcgac gcggccccgg accgccgagg cacacgtggt actgctcggt
     6061 gtccttctgc gtgaagcggg cgaggtgccg tgtctccggc gcgctcagct cggcgcgcac
     6121 gacctcggcg ccgggctcgg gccggaagtg cgtggcggcg agtgcggcga cgcgctcgtg
     6181 gtcgacgtgg ccgctggccg cgatgacgac gcccggattc acgtagtggg cgtcgtggta
     6241 gccgcgcacg gtgtcgaggt cgagcgcggc cagcgtctcg tagtggccga tgatgggccg
     6301 cccgagcggg tgctcgccga agacgacctc gcagaggtcg tcgtggatga gctcgggcgg
     6361 cgagtcctcg tacatcgcga cctcttcgag cacgacctcg cgctcctggt ccaggtccgc
     6421 gaacgagggt cgcgcgacca tctccgcgat gacctcgaac gccttgtcga ggtcctcgtc
     6481 caggagccgc gcgttgacca gcgtgtactc cttggaggtc gacgcgttgg gctccgcacc
     6541 catgtcgtcg aagatgtggg cgatctggga cgccgagaac ttgtccgagc ccttgaagat
     6601 caggtgctcg atgaagtgcg agatgccgcc gagctcgacc ggctcgagcc gggagcccgc
     6661 ggcgatccag accccgaggg ccaccgagcg cacggaatcc aggcgctcgg tggccactcg
     6721 caggccgttg tccagcgttg acaggcggta ggccttcgtg gcccgctccc tgctccgctt
     6781 tctctctctt attccgcctc gggcggcttg agcagcgtga gcccgatgcg gttgcgcgcc
     6841 ttgtcgatct cgacgacctc gacggtcacg gagtcgccgc gcttgacgac gtcctcgacg
     6901 ttcttcacgc gctcgccctt gggcgcgagg cgcgagatgt ggagcaggcc gtcggtgccc
     6961 ttcttgagct cgacgaaggc gccgaagtcg gtggtcttga cgacgcgtcc ggtgaacacg
     7021 tcgccgacct cgacctcctt ggtcatcgac tggatgcggt tcttgacgcc ctccgcggcc
     7081 tcctcgtcgg gggcgcagat gaagatcgtc ccgtcgtcct ggatgtcgat gtcggcgctg
     7141 aactcctcgg tcatgccgcg gatggtctcg ccgcccttgc cgatcacgag cccgatctgg
     7201 tcggggttga tctggatgct gaagatgcgc ggcgcccact ggctgagctc gctgcgcggc
     7261 tcggcgatga cctcggccat cttggcgagg atgtgcaggc gtgccttgcg cgcctgctcg
     7321 agcgcctccg tgaggatctc gaaggtgacg ccggtgatct tgatgtccat ctgcagggcg
     7381 gtgatgccct ccgcggtgcc ggcgaccttg aagtccatgt cgccgaggtg gtcttccacg
     7441 ccggcgatgt cggtgagcac gacgtagtcg tcgccctcct tgatgagacc catcgcggtg
     7501 ccggccaccg gggcggtgat ctgcacgccg gcgtccatga gcgagagcgt ggagccgcag
     7561 acgctggcca tcgaggacga gccgttgctc tcgaggatct cggacacgat gcgcacggtg
     7621 tacgggaagt cgagctcgct cggcaggacc ggcagcaggg cgcgctcggc gagggcgccg
     7681 tggccgatgt cgcggcgctt ggggccgcgc atgaagccgg tctcacccac cgagtacggc
     7741 gggaagttgt agtggtggat gtagcgcttc ttgtcctcga tgccgaggcc gtcgatgcgc
     7801 tggaactcgc cggtgccgcc gagcgtgagc aggctcatcg cctgcgtctc gccgcgggtg
     7861 aagagggcgc tgccgtgggt gcgcgggatg accccgacct cacagctgat cggccgcacc
     7921 tcatccgtcg cgcgaccgtc tggccggacc ttcttgacgg cgatgcggtc gcggatgatg
     7981 tccttctcga ccttggcgag tgcgcgcttg acctgcagga cgcgctccgg gtcggagtcc
     8041 tcgctcagga gctgcgcgac ggcggccttc ttgacgtcgg ccgtcgcgtc catgcgctcc
     8101 ttcttgccgg gcacctgggt ggccgtgtcg agcgctgcgg cgaagcccgt gcggacctcc
     8161 tcgagcacgc cctcgtcgat ggtcgtcacg gggacgtccc acttggcctt gccgacctgc
     8221 ttctgcagct cgagctgcac ggcgacgagc ttcttgatct cctcgtgcgc cagcttgaga
     8281 gcctcgacga tgacggcctc ggagacctcc tgggcgcccg cctcgaccat gacgatggcg
     8341 tcggcggtgc cggtgacgac gaggtccatg tccgactcgt cctggagctc ctcgagcgtc
     8401 gggttgacga ccagctggcc gtcgatgcgg cccacgtgca cggagccgat gggaccgccg
     8461 aacgggatgt tgctgatggt gagcgcggcg gaggcgccga gcgtcgcgag gatgtcgtag
     8521 gacacctcga agtcgacgga cagcgtggtg gccacgacgt ggatctcgtt catgaagccc
     8581 ttggggaaca ggggccggat cgggcggtcg atctggcgcg cggccaggat cgccttctcg
     8641 ctcgggcgcg attcgcgctt gatgaagccg ccggggatct tgccggcggc gtagtgccgc
     8701 tcttccacgt cgacggtcag ggggaagaag tcctgccctt cgcgcacgtc ttcgcgcatg
     8761 acggcgctga cgaggaccac cgtgtcgccg cagcgcacca gcacggcgcc gttggcctgc
     8821 tttgcgaggc gaccggtctc gagggtgatc gtctgacccc cgacttctac ttcaatcgtg
     8881 ttcatctctt ccttcgtctc tctgacccgg cgccccattg cgctcgggta ctctgtcgtc
     8941 tgcgtgggat tggcacgggg acgatgtcgc gggcgcgtct ggcgccggcg gggtgttact
     9001 tgcggagttc gagctccttg acgagcgcgc ggtagcgctc gaggtcacgc gcctgcaggt
     9061 agttgagcag gcggcgacgc tgaccgacca tcttgagcag gccgcgcctg ctgtggtggt
     9121 ccttcttgtg ggtcttgagg tgctcggtga gggtggtgat gcgctcggtg aggagtgcga
     9181 tctgtacctc gggggagcca gtgtcgccat cgtgcttggc gtgcttggcg ataatcgtct
     9241 ctttggcgtc tttggtcatt gccattcgtt ttcacctccc tcgtgggagc atccgggaac
     9301 ccagtcgcgg gttggctgcc cgtgaccgag aaagcggtcg cgctagggta gcacagcggg
     9361 cgggcggcga gaaaccgagc ccgcggagct ggcgcggtca ggcgccgccc gcgtccggcg
     9421 cgaggccgac ggcgtcgagc tcggggtcgg ccagcgagcc ggcggcggcg atgtcgaggc
     9481 gcatctgcgc gacgagcgcc tccgcgctcc cgaagcgtcg ctcgtcgcgg atcttgtgga
     9541 ggaagtcgag gcggatctca cgatcgtaga tgtccccgct gaagccgagg aggaacgcct
     9601 cgacggtcac gtgggtggtg ggctcgtccc ggcgctggaa ggtagggttg tgcccgatgt
     9661 tgacggcggt ccggtaccag cgcccgtcga cgaaggctcg cgcggcgtag acgccgcggc
     9721 ccgggaagat ggtgccggcc tccacgtcga cgttggccgt gggcacgccc agggcgcgcc
     9781 cgcgcttgtc cccgtggacc accactccgc ccgccgacgg cgggcggccg aggatctcgc
     9841 gcacctcctc gagctccccg tcgtgcagca gccgccggat gcgggtggag ctgatgggct
     9901 cgccgtgctc ggtgaccagc gcgcagacct ccacctcgta gccgttcgcg gcgccgcagg
     9961 cgcgcagctg cgccgtgtcg ccggcgccgc gcgcgccgaa gttgaagttc tcgccgacca
    10021 cgacggcgcg cgcccgcaac gcgccggcca gcaggccgtc gcagaactcg gccggggtga
    10081 gcgcggcgag ctcttcgtcg aacggcagca tgaccagctc gtccggaccg agctcggcga
    10141 tcagccgcgc cttctcctcg ttggacgtga gcagacgcgg cacgcgcgcg ggatcgatga
    10201 ccgccagcgg gtgccggtcg aaggtgacga ccgcgctggt gaggccgcgc tcgcgcgcga
    10261 gccgcacggc gcggcagatc accgcgcggt gacccgcgtg cacgccgtcg aacgtgccga
    10321 gtgcgagcgc ccgcggccgc ggcgccagcg acgcgatgtc ggcgacgacc tccatcaaag
    10381 cttgacgagc agcttgaggg cgttgccctc ctcggccggc ccgtagatgg cgatgagctc
    10441 gccgcggaac gtgagccgca ccggctcctg aggaccgccg ggcagacgcg cgccgttgcg
    10501 cacggcggtg acctgggccg cggagagctc gatgccgggc atgaactgca gcgccacggc
    10561 cggcggcacg aggccgggga tgtggtcgtc gccggcctcc ctctccccga tcgccgcttc
    10621 gaactcggcc agggtctggg cctcgtccag gcgcagatcg ccgacggcgg tgcgagagag
    10681 ctgctcgagg tgggcgccgg cgcccacggc ctcgccgagg tcgatggcca gctggcgcac
    10741 gtacgtgccc ttggagcagg cgatgcgaca gcgcatcgtc tgcgcggcct cgtcgaactc
    10801 gagcacgtcg atcgcctcga tctcgacctc cttgaccggc gtctcgacca cctcgccgcg
    10861 gtgagccttc ttgtagagcc gctcgccgtc caccttcacg gccgaggtca tgggcacgct
    10921 ctgcatgatg tgccccagaa agccggggat ggccgcgtcg accgcggcgc gcgtcgtcgt
    10981 cgcgccggtc tccgcgagct gacccgtgag gtcgcccgtg tccgagcgca cgccgaagcg
    11041 gacggtgcac tcgtattcct tgggcaggtc gacgaagaac cgcgccagct tggtcgccct
    11101 gccggcgagc accaccagca gcccggtggc gaacgggtcg agggtcccgg cgtggcccac
    11161 cttcttgacg ctcgggcgca ggttccgccg cacccgggcc acgacgtcgt gcgacgtgaa
    11221 gccctcgggc ttgtcgatca ggaggacgcc gtcgagaggc gccttgcgag ttcggaactg
    11281 agccacgcga tcacctcctc gggaccgtcg tcgctggaga acccggccgc cagcttgtgg
    11341 ccgccgccgc cttgcagggc ggcgacggcg ctgacgtcga tggtctcgga gcggaggctg
    11401 acgcgcacgc gcgcgccgcc cgtctgttct ttgacgaggg cggcgacctc caccccgccg
    11461 acgccgcgca ggctgtcgac gacgccctcg gtctcctccg gccccgcgcc ggtggccgcg
    11521 tagtccgccc gcgtgaccgc ggccagcagg gcgcggccgc cggctaccgc gcgcgcgccg
    11581 gcgacggccc gggcccagag gcgcagcgac gcctccgagc ggcgctcgta gagcagcgcg
    11641 tacatgcctg tgacgtcgac gcccagctcg accagccacg cggcggtgcg gaaggtggac
    11701 ggcgaggtgc tgtcgtggtg gaagtgaccg gtgtcgaacg agatgcccgc atacagggcc
    11761 gcggcggccg ccggggatgg ccgcagctcg agggcgcggg cgatgtcgca gacgagctcg
    11821 ctggtgctgc tcgcctcgcc gcgcacgaag gcgagctcgc cgtagcgggg gttgtcgtgg
    11881 tggtggtcga tattgaccgc gacctcgccc cagtccggca gggccaggcg gtccgggttg
    11941 ccggagtcga gcgcgtagag cgcggcgccg gagggcggcg cgccgcgcct gacgcgctcc
    12001 gcggggagca gcacgtcgcc gagcgggagc agctcgtcct ccgacacgta caggtgggcc
    12061 tcggcgccca gctgcgcgaa caggtcgagc atgccggccg ccgcgccgag cgcgtcggaa
    12121 tcgggcttct cgtggacggc gagcgcgacc cggctctcgc cgcgcatgcg ccggcagacc
    12181 gcatcgaggt cggcggcgag cggcttcacg cgtcgtcctc gccggccgga ggggtctcgc
    12241 cgccgtccgc cgcgtcggcg gcgccctcgt caccctcggc ctccggctcg gcgcccggca
    12301 ccgggatctc gtgcggctcc tcgccgagca cctcggcctc gcgcttgagc gcgcgctcga
    12361 tgcgcagggc gttgtccagg gtctcgtcgt agaagaactg caactgcggc gtgtgcctca
    12421 tgtgcagtga agcgttgatc ttggcctgca gaaagccgcg cgacttctcc agcgccttca
    12481 tggtgagctc gcgctcggtc tggccgccga gcacgctcac gaacaccttg gcgtgccgca
    12541 ggtcgcggct ggtctcgacc cccgtcacgg tcacgaaccc gatgcgcggg tctttgagcc
    12601 ccgcggaggt gatgaccgag gagagcgtct ccttgatgct ttcgttgacc tttaagagtc
    12661 gaagtcccat accgtgtcca ctgtgtcgga tgtcttgagc agcacgcgcg cgacctcgaa
    12721 gtcgcgcccg tgcaggtagc ggtcgatgtc gtcgaggcgc tcgcgggtct gctgcagcga
    12781 cgaggcggcg atcaccacga gcaccctcgc ccgctgccac gcgtcctgct gtcctacctc
    12841 gctgaacgag gcgcgaaacc gcgactgcag gacgtcacgc agcgaagcca gcggcgcgcg
    12901 cttctccttg agcgtccggg cctccgggaa gtgcagttcc gcgagataga cgccgaggca
    12961 ggcgtcaggc ctcacgggcg acttctcggg tctcgtacgc ctccagcacg tccccttcct
    13021 tgaggtcgtt gaagccgtcg agcaggatgc cgcattcgaa gccttccagg acctcgcgcg
    13081 cgtcttcggc gaagcgcttg agggaggtca tcttgccgtc gtggaccacc acgtcgttgc
    13141 gcagcacgcg cacgcctgcg ttgcgcacga tcttgccgtg cgtgaccatg cagccggcga
    13201 tggtgccgag cttggaggcc ttgaacgtgg tgcgcacctc ggcctgaccg agcacgatct
    13261 cctcgatggt cggcttgagc atgccgacca gggccgcggt gatgtcctcg gtgaccttgt
    13321 agatcacgcg gtaggtgcgg atgtccacgc cttcggtctc ggccagcgcg gtggccggcg
    13381 ggctcgggcg cacgttgaag ccgatgatga tggccgcgga ggcgctcgcc agcatgacgt
    13441 ctgactcgtt cacgccgccg acgccggagt ggatgatgcg caccttgacc tcggggtgct
    13501 ggatcttgag caacgcgtcg ctgagcgcct cgaggctgcc ctgcacgtcg gccttgacca
    13561 ccaggttgag atcgagcacc ttaccggcgg cgatgcgcgc gaagacgtcg tcgagcgtga
    13621 gcgcgtgctg cttggcgagc gcctcggtct tcaggcggtt ggcgcgcttc tgcccgaggg
    13681 cgcgcgcggc gcgctcgtcc ttgaccacac gacagaactc gccggccacc gggaggtcgt
    13741 cgaagccgat gatctcgacg ggagtcgagg gcccgccgct cttgagcggc tcgccgcgga
    13801 agtcgcgcat ggcgcgcacc ttgccgctgg cctcgccggc gacgatcgcg tcgccgacgt
    13861 ggatcgtgcc gcggttgatg agcatcgtgg cgacgacgcc gcggccggga tcgacccgag
    13921 actcgatgat gaacccgctc gcgggcgcct ccgggttggc cttgaggtcg ccgacctcgg
    13981 ccaccagcag gagcatgtcg aggaagttct cgaggccgat gcgcttcttg gcgctggctt
    14041 cgacgaacac gttgtccccg ccccactcct cgggcacgat gccgtgctcg gccaactgct
    14101 gcttgatgcg ctcgatgttg gcgtcgggct tgtcgatctt gttgatgacg atcacgatcg
    14161 gcacgccggc ggccttggcg tgggcgatgg cctccacggt ctgcggcatg acgccgtcgt
    14221 ccgcggccac cacgatggcg gcgatgtcgg tgaccttggc gccgcgggcc cgcatggcgg
    14281 tgaaggcggc gtggcccggc gtgtcgatga aggtgatgag ctggtcgctg tgcctgacct
    14341 ggtaggcgcc gatgtgctgc gtgatgccgc cggcctcgcc ggccgccacg gcggtgtcgc
    14401 ggatggcgtc caggaggctg gtcttgccgt ggtcgacgtg acccatgacg gtgatcacgg
    14461 acggccgcgg cttgaggtcc ttggggtcgt cgaccgcttc cttctcgagc tcggcctctt
    14521 cgtccgccgc gtgggtgatc tccacctcgc ggccgaagtc ggtggccagc acctcgatgg
    14581 cctcgtcgct cagcgtctgc gtgatcgtga ccatctcgcc gtagcccatg agggccttga
    14641 tgatgtcggg cgtggtgatg ccgagcagct gggagatgtc cttgaccgtg gacccggagg
    14701 ggaccttgac caggccagtc ggcacgaccg gcgcggtctg ctgctcctcg cggtcacggc
    14761 cgcgaccgcg accgcgtccg cggcgccggt cgcccgggcc gccgccgcgc cgggaggcgc
    14821 cggcgtcgag gatgacgcgg cgcttcttgc cggccggccc gccgccggaa cgccggcgta
    14881 cagcgccgcc cgcaggcgcc gcgtaggcct tctcgatagc ttcgtcgtcg acgctgacct
    14941 caccgtcgac gacctcgatg cccgccttct tgaggtgggc gagcacatcg tcgacactcg
    15001 tctttcgctg agtcgcgatg tccgagactt tacgtttcgc cattcacctc accgcctttc
    15061 gtcgtcctgg ggccgcgcga agaccgcacg gaccccggga tcgagtgtca ccgggccgcg
    15121 gaaggcgcgc gcgaacgcgc gcttcttctc cgcggcctcg agacaattct gttccggaca
    15181 caaataggcg ctcctcccgg gtgctcccgg tcgcgcctcc accacacgcc cctgctcggc
    15241 cacgagccgc acgagctcgt cctggccgcg cttgcggcga cagccgacgc acgtcctctc
    15301 cggcgtcgcc acggcgatca ctcctcgttc gtctccgcct cggcaggcgc cgccgcagcc
    15361 tcttccgtcg ccggcggggc cgccggctcg gcggggacgt agtcggcgtg gatgctgcag
    15421 taccgcgagc ccggctcggc ctggttggga cagcgcttgc cggtgcgcat gatcgccatg
    15481 cactgcccgt cgacgccgtc ctcttcgccg aactcgaggc tgttctcggc ctcggccatc
    15541 tgctgctggc tcttgatgtc gatacgccag ccggtgagct tgctcgccag ccgggcgttc
    15601 tggccttctt tgccgatcgc cagcgagagc tggtcgtcgg ggacgatgat ggtcgcggtc
    15661 ttctcctcgt cgtcgacgat cacctcgcgc acgcgcgccg gcgacagaga cttggccacg
    15721 aagcgcgccg catcctcgtt gtacgggatg atgtcgatgc gctcaccgcg cagctcgctg
    15781 accaccatgc gcacccgcga gccacgcggg cccacgcagg cgccgaccgg gtcgacgccg
    15841 tcggcgtgcg agatcacggc gatcttgcag cgcccgggga cgggcttgcc gtcgcgcccg
    15901 cggtaaccgg tctcgcgcgc cacctggcgg atctccacga gaccgtcggc gatctccggc
    15961 acctcgagct cgaacagctt gcgcacgagc tcctcgctgc gccgcgagag cttgacctgc
    16021 ggccccttgg cggactcctc cacgcggatg atcacggcct tgatgcgcgc gccgtgctcg
    16081 tagtgctccg tctcgatctg ctcgccgcgc ggcagctcgg cctcgacgcg gccgagctcc
    16141 accagcgtgt tgtgacgcgg gtcgctctgc tggacgatgc cggtgaccag ctcgcccacg
    16201 cggtcgatgt actcctcgta catgaggtcg cgctcggcct cgcggatgcg ctgcaggatg
    16261 acctgcttgg cggtcatcgc cgcgatgcgg ccgaagtcgt cgggcgtgac ctcgatgcgc
    16321 tcgacctcgc tcatgtcgac gccgctgagg tcgaggcgcc agttctcgtg ggtgtcgccc
    16381 gccggctgtc cctcgccgtg ctcctcgtat accttgaggg cctccgtgtc gacgtgctcc
    16441 gggaagatga gctcgtacac gcgcatgtcg ccggtctcgg tgtcgatctc gaccgcggcg
    16501 tactcggccg cgtccgggga cttcttgtac gcagaaagca gcgcgtcctc gagggccgcg
    16561 acaagagcct cgaaggcgat gcccttctcg ctctcgatct ggcgcagagc ctcgatgatc
    16621 tctctactca cgatggcctc cgtcggtttc atggttgtag accacgtgcg ccttgccgag
    16681 ggactcgagg ggcagcgtgg cgctgctccc tccctcgagg gtgagggtga tcgactggtc
    16741 gtcggcggcc gccaggcggc cgcggaagtt gcgccggccc tcgatgggcc gcgccgtggt
    16801 gaccgccacc tcgctgccga gcgcggcggc gaagtgcgcc ggcttgcgca gacgccggtc
    16861 cagccccgga gaagagacct cgagcgcata ccgctcgcgc agctcctcca gcgactggct
    16921 caccgccgcg cacaggtcga ggtcgacccc gcccggccgg tcgatgaaca gcgtcagcgt
    16981 cgaagccggc cggccccgca cctccacgtc gaccaggtcg acgtcgggga accgctcggc
    17041 gagcgcagct tcgatggtgg cttccagttc ctctctcgtc tccctcatac cgacccctaa
    17101 cacgagaaaa gtcggttgcc accgactttc acgcagagag cccgctcgtg gtgtgttgtc
    17161 ctgcccgatg agtatagcac ccgcttctcg tgccgttcaa ggacgccgcc ggagccgtcc
    17221 gcaggctcag gccggtgggg attctgccga tagtggaagg gaccagtcac acaccggcga
    17281 gaggcaggcg atgggagagt acacgacgtt cgagtgccgc cagtgcggct accaggccga
    17341 gcgcatccgc tggggcgtga gcgtctacga cccgcgccag cgcttcatgc ccggctactg
    17401 cgtcgagtgc ggcaccatcg tcgaggtcga catgaccggc gccgacatcc acgtggacga
    17461 gttccgctgc gtggtctgcg gcagcgagct gttcttcctc gagaaggccg cctcgtacac
    17521 ctgccccaag tgcaaggcgc acgacatggc gctgcaccag ggcgagtact ggtaagagac
    17581 gcctacttcg tgatgacgac cgggctgccg gccggcacgt acctgctgac cgccagcacg
    17641 tccttgtcgt gcatgcgcac gcagccgtgg ctgacggcct tgccgatgag ctcgtcctga
    17701 ttggtgccgt ggatcgccac cggtcctccc tgcggccagt ccggcagctt gggctggaac
    17761 gcgctgatcc cgatcgcgag caccccgtag gcgccgcccg gcgccggcgg cttgagcttc
    17821 tggttcacga agaaggaccc ggtcggcgtg ctgagcccgg gcttgcccac ggctacgggg
    17881 tacgtgccga tgagttcgcc gcgacggtac acgctgagcg tgcgctcgga gaggtcgatg
    17941 gtgatcctgg acgaggtcgt gtagaaggcc agcttgccct caggcaccca gccgcggctc
    18001 ccgttgggac gcatggcgag ccacacgcgg taccagaccc tgccccccgt ctctttggtc
    18061 gagtcgacca ggaccagcgt ggggtagccg ttctgattca ccttgccgag cttcgtcttg
    18121 accggcgccg acgtgctcgg acgcgtgtag accgtgacgg ggccgacggc tttgccgacc
    18181 gtccagcgct ggtactgcgg cgcgggatcg gcctcggcgg atgcggaggg cgccggcgtg
    18241 ggctgcgcca ccggcgccag cacctggccc gcgcccgacc cctgtgctcg ggcgcgcacc
    18301 gcggcgtacc cgcccagcgc cgccgcgagc agcacggcca ggccggcgac gacgatcacg
    18361 atgtcacgtg tgcgggagtt catcgacccc aaggatagac aaccggccca gacaggtaca
    18421 aacgagcatg tggtcgattc cccccgcagg cgtagactag tcggctgcaa ggggcaccca
    18481 acgaaaggag cgtcgcttga tcgccgtcat cgtcctcatc gtcatcgtgg tcgtgatcgt
    18541 cgccgtgctg gcgggcatgt acaacagcct ggtgaagcgc cgtaatcagg tggacaactc
    18601 gtggagccag atcgacgtgc agctcaagcg ccgccacgac ctcatcccca acctggtcga
    18661 ggcggtcaag gactacatgt cctacgagca ggagacgctc accaacgtca cccaggcgcg
    18721 tgccgccgcc atcgacgccg gcaatcaggg acccgcggcg gtggccgagg cggagaacat
    18781 gctcaccggc accctcaaga ccctgttcgc ggtcgcggag aactacccgg acctcaaggc
    18841 caaccagaac gtcatgcagc tccaggagga gctcacgtcg acggagaaca agatcgcctt
    18901 cgcgcggcag ttctacaacg acagcgtgct cacgttcaac aacaaggtgc agaccttccc
    18961 gtcgaacctc atcgccggca tgttcaactt caccacgcgg cagttcttcg aggtccccga
    19021 ggccgagaag gccgttccca ccgtcgacct gaggtagagg cgcggcctcg cggccgcaca
    19081 ccggcatgta cgaacagatc agccgcaacc gccgggcctc ctggctgctg gccgggaccg
    19141 tcgtcgccat cctcgcgatg ctgggcttcg ccatcggcta cgccaccatc ggcggcacgc
    19201 agggcggcat cgggctgctg ggcgtgttcg gcgtcgtcgc catcgcctgg agcatcatcg
    19261 gctactacac cggcgacaag atggtgctgg ccgtgagcgg cgcccgccgc gtcacccacg
    19321 ccgaggagcc gaggctcttc aacgtcgtcg aggagatgac catcgccgcc ggcctgccgc
    19381 agccgccggc ggtgtacgtg ctcgacgagg ccacgcccaa cgcccttgcc accggcaggg
    19441 atccgcagca cgcgtcgatc gccgtcacgc gcgggctgct cgaccgcctc gaccgcgagc
    19501 aactccaggg cgtgatcgcg cacgagatgg cgcacatccg caactacgac atccgcttcg
    19561 agacgctggt cggcatcctc gtcggcatga tcgccctcat cgccgatttc ttcctgcgct
    19621 ggagcttctg gggcgggatg agcgggcgcc gccgcggcag cgactcgggc ggcgaccagg
    19681 cgagcgcgat cttcgccatc gtcgcgctcg tgctcgccat catcgcgccg ttggccgcgt
    19741 acgcggtgca gttcgccgtc tcgcgccgcc gcgagtacct ggccgacgcg tcggcggtcg
    19801 agctcacgcg caacccgctc gggctggcgc gggcgctcaa cgacatcgcc gcggacccca
    19861 ggcccttgcg ccacgccaac cgggccaccg cccacctcta catcgagaac ccgatgaaga
    19921 agaccaagga cgtcacgggc gtcttcgaca cccaccctcc ggtccggcag cgcatcagga
    19981 tcctgctcga gatggcgcac gtcgggcccg aggcgctggt ggaggcgccg ccccgggcgg
    20041 gcgcaacccc gcccggaccc gtgacgccgg cgccccccgc ggccggtggg ccttgggtgc
    20101 ctccccctgg accgggaacg cccggctgag tcggcggcga cgctcgccga ggctcagtcg
    20161 acgccgtcga gccgccggcg gtaggtcgcg caggtcgggt ggagggcgcg ggccagccgc
    20221 aggtgagccc gcgcctcttc cccgcgcccc agtgcccgca gcgagcgcgc gagggcgtag
    20281 tgggcgtagt cgcagtcggg gacgtccgcc accacctcgc ggaacagcgc cgccgcgcgc
    20341 tcgtagcggc cgagcgcgaa ctcggcgcgg cccagcgtct cgcggatgga gttgcgcccg
    20401 ggctccagat cgagcgcccg ggagaggtac atcgccgcct gcgcggggtg cccgccctcg
    20461 agaaagccgg cgccgcggcg gaagaggctg taggcctcgc tctccgcgca gaagtcgggg
    20521 gtgcgccgct cgggtggctc cacggcgggc gtcctcagag gccgagccgc ggggcgatgc
    20581 cctgcatcgc ctcgagcccg agggcgaaga actcgtcggg ctcgaggccc agctcctcgc
    20641 agcgcgcgat cgcctcccgg tcgaccccgc gcgcgaacgc gccctcgcgg tagcgcttgc
    20701 gcaccgactt gaccttcacc ccggcgaggt ccttgtccgg gcggaccagg gccgcggccg
    20761 tgatcagccc gctcagctga tcggatgcga cgagcgcgac gtcgacgcgc tccgtcgccg
    20821 cgacgccggt gtccgggttg tgcgcgagga cggcgtggac ggcgtcctcg ggaaggccca
    20881 ggccgcggag cgtctcggcg gcgaccgccc cgtggcgggt gaagtcgtcc tcggtctcct
    20941 ccgcgtccag gtcgtgtccg aggccggcga ggccccacgc gtccgggtcc tcacccagcc
    21001 gtgcggcgag ggcgcgcatg atcgcctcgg tggccagcat gtggcggcgc aggttctcgt
    21061 tcgccacgtg tgcctcgacc tcggccagca ccagggcgcg ttcgggtgtc atcgcgtcag
    21121 tcctccctcg atctggtcgg cgatcgcccg cgcgcaggcc gtcttcgtgg tgcgggggac
    21181 gtggacctcg cccccgggcg tgaggatcgt gatctcgttc tcgtccgagc cgatcccgac
    21241 cccctcggcg aggatgtcgt tgaacacgag gaggtcgacg cccttggccg ccttcttcgc
    21301 ccgcgcgcgg tcgagccggg ggccggcctc ggcggcgaag ccgacgcggt agaggcccgg
    21361 gcgcgcgctg gaggcgagga cgtcgacagt gcgcacgagc tcgagcgtga gcgattcacg
    21421 ctcgccacgc tcgatcttgc cgccctccgc gtccttcgga cggaagtcgg ccaccgccgc
    21481 ggccatgacc agcacgtccg cgtcgcgcac ctgctcggcg accgccgcgg ccatctcggc
    21541 ggcgctctcg acacgcagca cgtcgtaggg cgcgccgtcg gcgggctgcg ttgtcacgag
    21601 cgcgacccgg gcgccgcgca ggtaggcctc gtcggcgacg gcgcggccca tcttgccgct
    21661 cgagcggttg ccgatgaagc gcaccgcgtc gatgcgctcg cgcgtcccgc cggaggtgac
    21721 cacgaccttc ttgcccgcga gcggccccgt ggccgcgccg gcgagcgccc ggcgcacggc
    21781 gagccggatg gcgtccggcg aggccatgcg cccggagccc tcctcgccgc aggcgagctc
    21841 gccctcctcg gtgggcagca cctccacccc gcgctccgcg agcacgcgga ggttctcgcg
    21901 cgtggccgcg tgctcgttca tcgcccagtt catggtcggc gcgacgatca cggggccgcg
    21961 gaaggcgagg tagatggcgg tgagcaggtt gtcggccatg ccgccggcca tcttcgccag
    22021 ggtgttggcg gtggccggag cgacgaccat caggctggcc gtcctggccg cctcgatgtg
    22081 ctggaaccag ccgccgctcg cctgccaggc gctctggtcg tcgaggaccg gggcctgcga
    22141 gacggcggcg aaggtgagcg gcatgacgaa ccgctgcgcc gcctccgtgg tcacgaccag
    22201 cggccggaag ccatctttga cgaggccgcg caccagctcg acggccttgt agatggcgat
    22261 gccgcccgtg acaccgacga gcacggtgcg ggcggcatgc atggtaggag cctcgctcac
    22321 ggccttggtc ttactcgctc gtctcgtggt cgatcttgtt gtcgacgatc tcctcgaagg
    22381 cgatcgtgag caggttggtg ctgcgcgtgc tgatgagcgg cggcgtgagc tcctcgagcc
    22441 cgagcgtgtt ctcgccgagg ctgtggtagt agttgttgat ctgccgcgcg cgcttggcgg
    22501 cgatcatgac gagctcgtag cgcgagccgt ccgcgtgggg cagcagctcg tcgatgcggg
    22561 gcttgtccac tcagtcttcc ttccgttgtg cttcgatgat ggcggccacc tcgccggcgg
    22621 cgcgcccggc atcgtcgttc accacgacgt ggtcgaactc ctccgcggcg gcgacctcgg
    22681 cctccgccct gtgcagccgc ttggcgatgg cggcggcgga ctccgtgccg cggcctcgca
    22741 gcctctccgc gagctcggcc atgctgggcg gggcgatgaa caccgtgatc gcctcaggca
    22801 tggccgcgcg caccgcacgg gcgccgatca gctcgatctc gaggatcacg tcgtcgccgg
    22861 ccgcgagctt ggcctcgacc tcgctgcgca gcgtgccgta caggttccca ctgtactcta
    22921 cccattcgag gaagtcgccc gccgtgacgg cggcgcggaa cgccgcttcg tccatgaagt
    22981 ggtactcgcg gccgtcggac tcgcccggcc gggccgcgcg cgtcgtcgcc gagacggagg
    23041 tcaccacgcg ctcgagtcgc gtgcggacga gggcgatgac ggtccccttc ccggcgcccg
    23101 agggccccga gatgacgaag acggacggga tcgttcagcc tccgaagtag gcgagcaact
    23161 cgtgccgctg gcgctccgag agaccgccca cggtcttgct cgggctgata cggcactgct
    23221 ccatgatgcg cgccgacttg acgcggccgc acttgggcgc ggccatgagg aggtcggcga
    23281 ccttggcggt gagcaggaac tccggcggat cggcgaggac ctcgttgatg ctcacgctgc
    23341 cgtgcttgag ctcggccttg agagcggcgc gccggctgcg gacctcgttg gccctgccca
    23401 gggcgtccat gcgctggtcg agcgaccgct ccggggctgt gttgaattgc ttcgacgagg
    23461 gggacatcgc gcgagagtat atgtcgggac ggaccgcgcc tgcaaggccg ctccagcctc
    23521 cacacggtca cgcaaagtcc ctccatctcc accacttttc gcggtcgtgc cgggagatgc
    23581 gccggcggat cggtcagcac cgctgaggtg gtgccgcctc cgagcaccgt gtgtgcccgg
    23641 gccaacggcg cgggtcgcgg ctccggtccc tcggggaaca taccccacaa agctgaccac
    23701 gccgacagac cggcagcgcc gacacacgat cgctggagga atggtcatgt ctcccgaatg
    23761 cgcctgtcgc agcgaccagc tgagcgggtc acgcgcgctg gtcaccgcca gcggcgaact
    23821 ggatgtcaac acctgcccgc agttccagaa ggccatcgcc gccgctgcgc gcagagacgc
    23881 cgagcacctc ctggtcgacc tttcggacgt caccttcatg gactcgaccg ccctcggcgt
    23941 gctggccgcg gagcagcgtc gccggtcgga gccgctgcat ctcatcgtga aggagcgaca
    24001 gttgcagcgg cttctcgagg tgacggggtt cgtcttcgtc ttcgccgtcc actcctcgct
    24061 ggaggaggct ctgctcagga ccgggcgtct tcaagcggag cgagcacgcc cggtcgatct
    24121 cgcacccgcg cgggatcgtc cgtgaggctt cgaggcgcgg ggcgcggcgt caggcgcgga
    24181 gcgccgcgcc ccgcgcctcg gcgaccgacg cgaacccgtg ctcccgcagc tcctcgcgga
    24241 gctccgcgag gatgcgctgc ggcgcctcga aggcggtgaa gttggcggcg ccgaccgcca
    24301 ccgccgtggc gccgcaggcg atgaactcga gcgcgtcgcg cccggtcatg acgcctccca
    24361 tgccgacgag cggcgtgtcc gggaaggcct ccgcgacctc cgcgaccatg cggagcgcga
    24421 tgggcttgat cgccgggccg cagagcccgc cggtgcggtt gccgaggaac gggcgcaggg
    24481 tgtggcggtc gagcaccagc gccttgaagg tgttgacgag cgacagcccg tcggcgccgg
    24541 cgcccagcgc ggcagcccct atctcggtga cgtcgcggac gttgggcgtg agcttggcca
    24601 ggaggaagcg gccggtggcc gcgcggcagg cgccgacgac ctcctccgtg gcggcagggt
    24661 cggcgccgat ctgcagcccg ctcgagacgt tggggcagga gatgttcagc tcgtagccct
    24721 cgatacgcgg cgccgccgcc ggcggcgccg cagcgaggtg cgcctcgagg gcgccgatca
    24781 cggccgcgta ctgcgcggga tcgttgccgc cgacgctgac gacgaccggc tgccggagcg
    24841 ccgcccactc ggcgagtccg ccgatccagg cgtggatgcc cgggttctcc aggccgatgg
    24901 cgttgatcat gccggccgcg gtctcggtga cgcgcggcgg cggattgccg gtccgcgcgt
    24961 cggccgtgac cgtcttgggc acgtacgccg cgaacgggaa atcggcgaag tagtcgccgt
    25021 cgtagcgccg cgcgtactcg aggaggtcga acgtgcccga ggcgtcgacc agcgggtgct
    25081 cgagctcgat gggcccgagc cggacggaga gctcgggtga cgccgcggtc acggcgtgac
    25141 ctcgtccagg cggaacacgg gaccctcgct gcacacgcgg accttgccgc gcgtcgtgtc
    25201 gaccacgcag ccgtggcacg agccggtgcc gcaggccatg tgcgcctcca gtgaaacgta
    25261 gccacggagc ccctcggcga aggtccactg ctgcaccgcg gcgatcatgg gcgtggggcc
    25321 gcaggcgtac accgtggtgc cgggggcgac gtcgagcgcg gcgagcaggt gcagcgccgt
    25381 acccgccttg cccacgctgc cgtcctcggt ggccacccag agccggtcga tgcggaaggc
    25441 gccggcggcg cgggcggcgc ggaagtcgcg gaagccgaag gcggcggtga cgtgcgcccc
    25501 cgccgccgtc agctcatcgg ccagatactg gagcggggcg cagccgatgc cgccgccgac
    25561 gagcagcgcg ttggtgacgc cctccgtggg gaacgcgctg ccgagcggcc cggcgacgtc
    25621 cacgccgtcg ccgacgtcga gctgggtgag acgctcgctg ccgtcgcctc tggcctcgac
    25681 gaggaggctg atgcggtcgc cgtgcacggc gaagagcgag agcggccggc gcagcaggaa
    25741 cccgcccccg ggcacggcga ccatgacgaa ctgtccgggg gcggcgcgcc gcgcgacgcg
    25801 cggcgcgatg agctccacgg ccgcgaacgc cccgaagtgg cgtacgccgg cgacccggca
    25861 gtgggtggtc tccgcccact gggagtggtc gccgtgcatc tgtgccggtg cgccgtccat
    25921 ggtcagtccg tcgccgcgtg caggtcctgc agacagtgta cctgcacccg tggccgctgg
    25981 agcgcctcga tcgccgacac cgccgcggag gcgccggcga gggtggtgat ggaagcgatg
    26041 cccttgcgca gggcggtcgt gcggatctcg tacccgtcgg cgcgcgcccc ccggccgaac
    26101 ggcgtgttga tgacgaggcc gatctggccg ctctcgatga ggttgaccac gttgggctcg
    26161 ccctggaaca ccttgttcac cacattgacg gggatgccga ggctggtgag cgcagtggcc
    26221 gtacctctgg tggcgtagat cgcgaacccg agcatgtgga gccgctgcgc gaggatcgtg
    26281 gccgccgact tgtcgctgtc gcagaccgag aggaacacgt cgccctcggt cggcaacggc
    26341 atgcccgcgg cggcaacggc cttgccgaag gccgccggga acgcctcggc gatgcccatc
    26401 acctcgcccg tcgacttcat ctccgggccc aactgcgtgt ccgtgccggg gaagcggttg
    26461 aacgggagca cggcctcctt gacgctcacg tactcgggca cgcgccgctc gaggcccata
    26521 gccttgagct tctcgccgag gatgacgcgt gtggcgatct gggccatggg cacccccgtc
    26581 gctttgctga cgaacggcac cgtgcgcgag ccgcgagggt tcacctccag cacgtacagc
    26641 tggtagctct ggtaggcgaa ctggacgttg atgaggccct tgaccccgag gcccagcgca
    26701 agggccttgg tctgcttgcg cacctgctcg agcgtgccct cgccgaggga gatggacggg
    26761 atgacgcagg cggagtcgcc cgagtgcacg ccggcctcct ccacgtgctg catgacggcg
    26821 ccgatgtaga cgtcctcgcc gtcgcagatc gcgtccacgt cgacctcgat ggcgtcctcg
    26881 aggaacttgt cgatgaggac ggggtgctcg gggctcacgc tgacggccgt ctgcatgtag
    26941 tgctcgagct ccttggggcc gtagacgatc tgcatggcgc ggccgccgag cacgtacgac
    27001 ggccgcacga gcagcgggta gccgacgcgc gccgccacct ccgcggcctc ggcggccgtg
    27061 gtcgcgaggc cgtactcggg gcagcgcacg ccgagctgct cgagcaggcc gccgaagcgc
    27121 ccgcggtcct cggcgaggtc gatggactcc tggggcgtgc ccaggatcgg gatgccggcc
    27181 tcggcgagcg cgcgggcgat cttgagcggc gtctggccgc cgaactgcac gatgacgccg
    27241 cggggccgct cgttggcgac gatgccggcg acgtcctcga aggtgagcgg ctcgaagtag
    27301 aggcggtcgg agatgtcgta gtccgtgctg acggtctccg ggttgcagtt gaccatgacc
    27361 gcctcgaacc cctgctcccg ggcggtgagc gccgcgtgca cgcagcagta gtcgaactcg
    27421 atgccctggc cgatgcggtt gggtccggag ccgaggatga cgacgcgctc cctctccccc
    27481 ggctccgcct cgttctcctc ctcgtaggac gagtagaagt acggggtctc ggcctcgaac
    27541 tcggcggcgc acgtgtcgac cgccttgtag accggcagga cgtcgtgctc gaggcgcagc
    27601 gcgcggacct cgcgctcgcc gcggccggtg agctcgccga ggcgcttgtc gctgaagccg
    27661 tagcgcttca tgcgccgcag cagctcgggg gtgaggcgcg gcgcctcgcg cacctcgcgc
    27721 tcgagctcga cgatgtcggc cagctcgctg aggaagaacg gggagatgcc ggtgagctcg
    27781 tggacctcgt ccacgccgag cttctggcgg aacgcgtgca ggaccagctc gtagcgttcg
    27841 gccgtcggga tggccagggc cttgcggatc tccggcgccg accccggaag atcggtcggc
    27901 gcgtccagct cgcgcgagcg cacggccttc tggaacgcct ccttgaaggt gcggccgatg
    27961 gccatcgcct cgcccacgct cttcatgtgc gtggtgagct cgcggtccgc gagcgggaac
    28021 ttctcgaacg cccagcgcgg catcttgacg accacgtagt cgagcgtggg ctcgaagcag
    28081 gcgggcgtct tgcgggtgat gtcgttgtcg atctcgtcga gcgtgtagcc caccgccagg
    28141 cgggcggcga tcttggcgat cgggaagccg gtggccttgg aggccagcgc cgagctgcgc
    28201 gagacgcgcg ggttcatctc gatgaccacg acctcgcccg tctccggatt caccgcgaac
    28261 tggcagttgc tgccgccggt gtcgacgccg atcttgcgga tgatcgcctt ggaggcgtcg
    28321 cgcagctcct ggtactgggc gtcggtgagc gtgagcgccg gcgcgacggt gacgctgtcg
    28381 ccggtgtgga cgcccatggg gtcgatgttc tcgatggagc agatgatcac gacgttgtcg
    28441 gcgcggtcgc gcatgacctc gagctcgaac tcgtgccagc cgacgacgga ctcctcgagc
    28501 aggacctgcg agatcgggct gagagcgagc ccctgctcgc agatacgcgt gaagctgtcg
    28561 tggtcgtagg cgatgccgcc gccgctgccg ccgagcgtgt acgcggggcg gacgacgagc
    28621 gggaggccga gctcggcgca ggcggtctcg gcctcgtcca tggtctcggc gagcatgctg
    28681 cgcggcacgc gcaggccgat ctcctccatg gcggtgcgga actcgaggcg gtcctcggcg
    28741 cggtggatgg ccttctcgtc ggcgccgatc atctcgacct cccagaggtc gagcaccccg
    28801 gcggccttga gctccatggc caggttgagc gccgtctgcc cgccgagcgt cggcagcagg
    28861 gcgtccgggc gctcgacctc gatgatgcgc gtgagcgtgg cgaggtcgag cggctcgatg
    28921 taggtgcgcg tcgcgaaggc ggggtcggtc atgatcgtcg ccgggttgct gttgacgagc
    28981 acgacctcgt acccctcgtc gaggaggacc ttgcaggcct gcgtgccgga gtagtcgaac
    29041 tcgcaggcct gcccgatgac gatgggcccg gagccgatga tgaggatctt gtggaggtcg
    29101 tcgcggcgcg gcatcaggcc tcccctcccc gcttgaacct gtcgatctcg tcgcggaagc
    29161 ggtcgaacag gtagagcgag tcgtgcggcc cgggggtcga ctcggggtgg tactgcaccg
    29221 agaacgcgta ccgctccggc gcggccacgc cctccaccgt gccgtcgttg agattgacgt
    29281 gggtgatctc cgccccgggg acctgctcgt ccttgaccgc gaacccgtgg ttctgggtgg
    29341 tgatctcgat gacgccggtg cgcaggtcct tgaccggatg gttggcgccg cggtggccga
    29401 acttgagctt gaaggtcttc atgccgagcg cctgcgccag gagctggtgc ccgaggcaga
    29461 tgccgaacac cggcaccttg ccgagcaggc gctcgacggc cttgaccgcg taggtgacgg
    29521 cggccgggtc gccgggcccg ttgctcagga acacgccgtc cggctccatc ttgaggacct
    29581 tctgggcgct ggtctgcgcc ggcaccaccg tgacgcggaa gccgcgccgc gccaggtagc
    29641 cgagcatcga gcgcttgatg ccgtagtcgt aggccaccac gtggaggtcg ggcgccgggg
    29701 ccccttcggg cgccggcgcc tcgtacgccg tctcacaggt gacgaccttg gcgaggtcga
    29761 gcccgcccat cgccggcaac ccctgcgccg acttgcgcag gtgcttgacg tcggcgacgt
    29821 cggtgctcac gaccgcccgc agcgcgccct tctcccggat gtgccgggtg agggcgcgcg
    29881 tgtccacccc ggtcacggcg aggacgccgt gctcggccag ccagtccagc cacgtctggc
    29941 gcgaggcgcg gttgaagcgg tagttggtga gctcgcgggc gatgatcgcc cgggcgtgag
    30001 ccttgccgga ctcgtcccgc tcggcgtcgg ccccgtagtt gccgttcatc gggaacgtgt
    30061 acgtgatgag ctgaccgcag tacgaggggt cggtggcgat ctcctggtag ccgcacatgc
    30121 tggtgttgaa gacgatctcg ccgcccaccg agcccgagcc ggcgagagcc tcgccggcga
    30181 aggtggcgcc gtcctccagg acgatgacgg ccggactagg caaggtcgtc ccaggcgacg
    30241 cgcccggcgg ccaccgtgag gcggaccttg ccggtgagct cccgccccag ccagggggag
    30301 ttccaggagc gcgactgcag cgcctccggc gtgaccgtcc agacggccgc cgggtcgacc
    30361 acgcagaggt cggcggtcgc gccggcggtg agcgacggcg cctccagacc ggcgatgcgc
    30421 gccggcgcgg agctcatccg ggtgaccagc gtcgcgaggt cgagctcgcc gctcgcgacg
    30481 agctccgcgt gcaggacgct gaaggcggtc tccagcccga tgcagccgaa cggcgcctcc
    30541 tcgtagggca cgtccttctc ctcggagacg tgcggcgcgt ggtcggtggc gacgcagtcg
    30601 accaggccgc tcctgagcgc ctccacgagc gccttgcggt cgctctcggc gcgcagcggc
    30661 gggttcatct tgaggttggg gtcgagcgca gcgacgcgct cgtcgttcag ggtgaggtgg
    30721 tgcggggtgg cctcggcggt gacccgcacg cccgcctcct tggcgcgggc gatggcctcg
    30781 agcgacgccg cggtgctcac gtggcacacg tgcaggtccg cgtcctcgta ggcggcgatg
    30841 tccagcgagc gggagacgtc gaggctctcg cagagcgagg ggatgccggt gaggccgagg
    30901 cgcgccgacg ccgggccctc gtgcatctgt ccgcccttga agagcgagtc gtcctgcgcg
    30961 tggatggcca cgaagcgctt gctcaccttg acgtactgga gcgcccgccg ggtgatggcc
    31021 gccgtgggca gcgggcgccc gtcgtcgctg aaaccgacgg cgccggcggc gccgagctcg
    31081 cccatctcgg tgagctcctg cccctcgaga cccttggtga cggcggcgaa gaagccgacc
    31141 gggacgaccg cgtccgcccc ggcctgcagg gccaggcctt tgagcaggcc ggcgttgtcg
    31201 acgaccgggt cggtgttggc catgccgaag gcggcgacgt acccgcccgc cgcggccgcc
    31261 gccgaggcgg tcgcgagatc ctcttcgtcc tcgcgccccg gcgtgcggaa gtgcgcgtgc
    31321 aggtcgacga agcccgggag gaccagcatg ccggcggcgt cgaccacgcg cgtgttctta
    31381 ccggccttga ggcccttgcc gacctcggcc accttgccct tcttgacgag cacgtcctgg
    31441 accgcgtcga cgcccgcctc ggggtcgacg acacgcccgc cggtgatcag cagctcggcg
    31501 gccgggagag gcttgcacca cgtccgttcc atcagagcac ctccccactc gcagccccgt
    31561 cgctgtagcc gacgatggtc cggtacagga tcgccatgcg caccgcgagg ccgctgaaga
    31621 cctggtgggc gatcaggttc tcgtcgtcgt cggcgatgtc cgaggcgatc tccacgccgc
    31681 gattgatcgg gccgggatgc atgacccgct gcttcggccc cagcctcgcc tgcgagatgc
    31741 cccagagctg cgcgtactcg cgcagcgaag gcaggtaggc cgagcccggc ttgatccgtt
    31801 ccttctggat gcggagcagg tagatgacgt ccgcctcctc gaggtcggag aggtcgtagg
    31861 cgacgttgac gcccatctcc tcgatgccgc gcggcatcat cgtcggcgga cccgccaggg
    31921 tgacgtccat gccgagcatc tggaagccca tgatgtcgct gcgcgcgacg cggctgtgca
    31981 ggatgtcgcc gacgatgaag acccggagcc cgtccagctc gcggcccagg ttctggcgca
    32041 tgctgaagag gtcgagcagg cactgtgtgg gatgctggtg cttgccgtcg ccggcgttga
    32101 tgacgctcgc ctcggtgttg gccgagagcg tctggcaggc gccggcgctc gggtgccgca
    32161 tcacgatgat gtcgggcgag taggcgctca gcgtgatcgc cgtgtccttg agcgtctcgc
    32221 ccttctcgac ggccgagccg ctggacttga gggccagcgt gtcggccgag aggcgcttgg
    32281 cggcgaggtc gaagctggag gacgtgcgcg tcgacgcctc ccagaagagg ttgatgatgg
    32341 tgcgcccgcg cagcgtcggg accttcttga tgtcgcgctc gagcaccggg agaaagctct
    32401 ccgcggtgtc gagcagcagt tcgacgtccg ccctcgagag gtcctcgatg ctgatcaggt
    32461 ctcgcttggg cattggtcac gcctccttgc gcacgaggac gacctcgtcg acgccgtcga
    32521 tctcttcgac ccgcacgctg atgcgctcgc gtgcggccgt gggcaggttc ttgccgacgt
    32581 agtccggccg gatgggcagc tcgcggtggc cgcggtccac gaggaccgcg agctgcacgg
    32641 tcgccggccg cccgtagtcg aacagggcgt cgatggcggc gcggatggtg cgccccgtgt
    32701 agaggacgtc gtcgacgagg acgatccgcg ccccgtcgat ggtgaacggc aggtcggtgt
    32761 ccttgacgac cggctgcggc gtgctcggga tgacgccgac cgcgccgcgc gcgctgacgt
    32821 cgtcgcgata gaacgagatg tcgaggttgc cggtggcgta ctcgcgcccg ccgacctcgc
    32881 agagcagctt gtggatgcgc gcgccgagga aggcgccgcg cgtgtggatg cccaccacgg
    32941 cgagcttctc ctcgccgggg ttcttctcca ggatctcgtg ggcgatgcgc gtgatggtgc
    33001 gcgacatctg ctcgcccgtc aggacgacgg tctcacttgc gggtctgggc tgaggcataa
    33061 aaagagcgcc gccggcacct gatggcgtac ggcaacgctg cacccccttt ccgtctctcg
    33121 ggacgtggat taaaggtcga ggcgtactct agcagccgcc gcggggcggc ggcaaggcga
    33181 gccgcggcgg cgttcaggcc tcgggctgcc cgggcaggcc cagggcctcg aggaaggcgg
    33241 cgaggtcggg cgggagcggg ctgcagcagc ggatctcccg cccgtcctcg gggtgcggga
    33301 aggcgagcct cgcggcgtgg aggaactgcc gcccgaggcc gtccgggcgc ggcgcccggc
    33361 cgtactgcac gtcgccggcc accgggtacc cgagcgcctg gaagtgcacc ctgatctggt
    33421 gcgtcctgcc cgtctccagg cggacgcgca ccagcgtgta ccccggcgcc cggcccagca
    33481 cctcgaagtg cgtcacagcc gggcgtcccg cggccgtgtg cagcgacatg cgtttgcggt
    33541 cgcgcaggtg ccggccgatc ggcgcgtcga tggtcccccg ggcctgcggc aggtcgccgc
    33601 tgaggagcgc gaggtaggtc cggtggatgt cgcgcctctc catggcggcg acgagacgcc
    33661 tgtaggcctc gtcacgacgc gcgacgatca gcaggccgga ggtgtccttg tcgaggcggt
    33721 ggacgatgcc cgggcggagc tcgtggccgc cccgcgcccc ctccgccacg aggccctgcg
    33781 cgagcgtccc gtgctcgtga ccgggcgccg ggtgcaccac cacgcccgcc ggcttgtcga
    33841 ccaccagcag ccagccgtcc tggtagacga cggtgaaggg caccggctcg gccgccaggg
    33901 tgaccgcggg cggcggcgga gcctgccaca ccacctcttc cccggcgcgg aggacgtggt
    33961 tcttgcgcgc aggggcgccg tccaccgcga cgaggccgcc ggcgatgagg cgctgggccg
    34021 cggcgcggct cacgccggcg tgcgcggcga gcgcgacgtc gagacgctcg cccgcgtccc
    34081 cggccgccgc gcggaacgcg cgcgcttcac tcctgccggc caacgaccac ctgcagcgcg
    34141 aggaggaccg tccccacgac gatcgccgcg tcggcgacgt tgaaggacgg ccagccgtag
    34201 aagccgatga agtcgaccac ggcgccgtcg aacctgagcc tgtcgatcag gttgccggcg
    34261 gccccgccga ggatgatgcc gagcgcgggg cggtacctgg gcgggctgag gaccagcgcg
    34321 acggccacgc cggcggcgac ggcggcgacc agcaccacca cgacgtcgcc ggctccggcg
    34381 aaccggctga acgagatgcc ggagttgtag gtcaggtcga tccgcaggcc gccggccagc
    34441 tgccggggca ggtgctcggc ctgggcccgc accacccact tggtcgcctg gtcggcgacc
    34501 acgacggccg cggccaggat cagcgcgagg acgtaccccc ggttacttct tgccacgcgg
    34561 cttgggcacg gcgtcgagct gcttgatgtc gacgtggccc atcgggcacg tcggctcctt
    34621 tgccttgccg ctgcgtccac cgtcgttgac gacgatgttc agaggatagg tcgccttgca
    34681 ctgcttgcag taggcaagct tggccatcgg gtactccttt gctcggtcag agggcgagtc
    34741 tacgcctcgc ctcgtcgagg gtcactccgg tcaggcgcag cagctttctg cgtgagcgcc
    34801 ccccggcgac caccgtgacg tcctggcgcc gcacgcccag ctcccgcgag aggcaccgta
    34861 tcagctcgtc gttggcgcgt ccctcggacg ccggggcggc gagacggacg cggaggcgcc
    34921 cgtcggcgac ccccgccaac tcgctgcgcc gcgcgccggg cgtcacccag acggagaggc
    34981 tcaggccgcc gcgcgcctcg gcgagcggcc cgttgtcacc acttgaactc gtcctcgttc
    35041 accccggtgt ccacgtcggc gagcagatcg tcgctctcgc cgaagaggat gcggctgcgc
    35101 cgcttctgcc gaccttcgtc atccggcgcg ccttcggggg ccgggtgcgc ggcggtcacc
    35161 tcgggatcgt cctcccgggg gcggtccggc cggccctcgc cgtccgaggg agccggctct
    35221 gtatgcacgc ccgcctgcac gccactccgt ccgcgctgcg gtacgactgc cggttctccc
    35281 ggaatgacgt accgccgctc cggctcgggc tcgggatcag gctcatgctc atcctccgtc
    35341 gccgcaggcg gatcgggcgc ggcgacgggc tcttcggccg gcacgggcgc gtcggcgacc
    35401 ccctcggccg gctccggcgt cacggcgctc ggcgccgggg tgggctcggg ccgcgccggc
    35461 gccgcctccc gggcgatggc ttccttgatc gccgccgtgt gccgcgagaa gtccgacgcc
    35521 ggcggcgcct cggctacctc cggcgcgtcc tgcagttgcc gcaggtaccc atccagcagc
    35581 tgccggaact tgaagcggaa gtcctcctcg gcgctgcgca gcttggccat ggactgctcg
    35641 atcgcctggc gctccgagta cgcctcgttg acgagctggc gcgccttgag ctcggcgtcc
    35701 tgcaggacga gctgcgcctg cttgttggcg ttctgcttct gctcctcggc gctcgcctgc
    35761 gcgttgacca gggtcttctg cagggtctcc tcgatgcgcc tgtagccggc gaccttctcc
    35821 tccagcgact ccacgcggtc ctgcaggtcg atgttctcct tgaacagacg ctcgtactcg
    35881 tcggcgatct ggtcgaggaa ctcgtcgacc tccacgtcgg cgtagccgcg catgccgcgc
    35941 ttgaactcct tgtggcggat gtcgagcggt gtgagcttca tcgtctcccc tctcggtcgc
    36001 gcggccttgc ccgcgcgtcg tctctgctct ccctagtgtg cgacgcggct acgcgagttc
    36061 cttggcgcgc tcggccgccg cctgcacggc gagcgcgacc gcctccgcga cgtccctctc
    36121 ctcgagggcg gcgatcgcgg ccgccgtcat ccccccgggc gtcgccgccg aggcgatcac
    36181 cgcgtcggga tcgccgtcgt gggagatgac cgcggcggcg ccgtggacgc cggcgatcgc
    36241 gagccggcga gctacgtccg gcgcgaggcc gcgccgctcc gcggcgcggc cgaagtcgcg
    36301 cacgagcagg gcgagcatcc cgggcatgca cccggcgacg gcggtcgcca cgtcgaagtc
    36361 ccgctcgtcc agctcgatca cgtccccggc cagggcgaag agctccccca cctcggccgt
    36421 gcgctccgcc agggtgcccg gcacgaacag gaagaccccc aggccgagcg ccgcggcgac
    36481 gttgggcatc acgcgtgcca cgcgggcgtc gccgggcagc gccgcgcgca gctccgcgag
    36541 cgggacgccg gcggcgacgg acacgagcgg gcggtcgccg agcagcgggc ccgcctcggc
    36601 gagcacgccg gcgacgtgct gcgggcgcac cgccacgacc acgacgtcgg atgtcgcgat
    36661 gaggtccggc agtgacgcgg cgagcgacgc cccggtgtcc gcggcggcgt gcgccgcggc
    36721 ctcggcggag acgtcgagat acgtgagccg cggtggctgg gagacctcgg ggcgggccca
    36781 tccccgcccg agggcccggg cgatgtgccc ggcgccgacg cacccgaccc tcacggcgtc
    36841 acgcgggcgc acgccggccg ggtcacgcgg ccgggacggc cgcgccgcac ctcagaactg
    36901 attgaagaag cccttctcaa tgagcctcgc ccgctcctct gcggagacct cgacgttgcg
    36961 cggggtgagc aggaagatct tctcgccgac acgctgcatg cccccgtcga gggcgtaggt
    37021 gaggccgctg cagaagtcga tgagccgctt ggccagctcc gtgtccgacc cctgcaggtt
    37081 gatgatcacc gggacgtcga ccttgaactt gtcggcgatc tgctgggcgt cgttgaagtt
    37141 cttggggatg acgaggtgga cctcgacgcg cgccgggcgc gccggctcga gggcgcgcac
    37201 cgccggctgc gacgaggcgg gcgcgcgacg acgcggctcg tcggcgaaga tgtcgtcgta
    37261 gtcgttgcga cccttggcgc tgttgagctt gcggacgttc gggcgctcct ggtacgagcg
    37321 ttcgagctcc tcgtgcgggg cgagggtctc gtcctcgtcg tagtcgtcct cgtcatcggc
    37381 gatgccgaag tagaccgctg tcttgtgcca ccagtcgcct aggcccatca ggtccctccc
    37441 tacagggtca gaagagcacg ctcccgacgc ggacgatggt cgccccttcc tggacggcca
    37501 cttcgtagtc ggcgctggtg cccatcgata gctgctcgaa agtatacgtt ccgctccacc
    37561 tcgccgaaag ccgttgggcg agctcgcgcg cgcgcacgaa gaaggggcgc gcctcctccg
    37621 cgtcgtcgaa gagcggcggc atgcacatga ggcccgtgaa gcggaccttc ggagaagcgg
    37681 cggcggtctc gaggaaggcg tcgacctccc cgagcgccac gccgctcttg ctctgctctc
    37741 caccgatgtt gacttccagc agcacgtcgg tggcgttcgc ggcgcgcgcc tcgatctcct
    37801 gcaccacgct cgtgctcccg acagagtgga tgagactgac gatcggcagc accagccgtg
    37861 tcttgcggct ctgcaggtgg ccgatgaagt ggtagtcgaa ggcatcgccg tacagctcgt
    37921 gcttggcgac gaggtcctgg gcgcggttct cgccgatgag gcggatgccg gcgtcgcgca
    37981 gcgcgcccag gtgctcggcg gccacgtact tggtggcgac gagcacgcgc acctctgccg
    38041 ggtcgcgccc ggcggccccg gccgcctccg cgacccggct ctcgacctcc gcgtagcgct
    38101 cgcgcaccgt ctccacatct gggacgaact gtcgtaccac tgctgcctcc tcggcggcct
    38161 acttctcgaa caggcctacg cttcctgccg ccatgctacc gccagatgcc gtccggtgac
    38221 cccgcggtcg cggcggtgcg agaagaagcg cgcgtccgac gcggtgcaga gaccggcgac
    38281 ggcgacgctc cgcgcgggca cgcccgccgc ctcgagctcg aggcgggcgc agcgccagag
    38341 atccacggtg gcggcgccgg cggagcccgg gaaccgggcc tcgaaccggc gccgcaggct
    38401 ctcgtccacg gtgaagcagc acgggccgat gctcgggccc accgccgccg cgacggggcg
    38461 gcggccgctc cgggcgagct ccgcggccgc ctcgccgacg atcccggcga gcatgccgcg
    38521 ccagccggcg tgcacggtcg cgtaggcggt cccttcctcc ccctcggcga cgatcaccac
    38581 ggggacgcag tcggcgtagc tgatggcgag gccgagcccc ttcgctcggg tgagaagccc
    38641 gtcgtgctcc ccgatcacgg tctcgacgtc gaacgccccg cgcccggcct cgtcctcgcc
    38701 gacccagcgg agatcggtgc cgtgcacctg tccggggacg acgaggcgcc ccggcgacag
    38761 gcccacggct gcgcacaggc gccgccggtt ctccagcacg tcctcggcac ggtcctccgt
    38821 ggacaggccg aggttcaggc tgtcgaaggg cggcgccgag acgccgccgg tccgcgccgg
    38881 gaacgcggcc gtcacgccgc ccgcctccca cgcgagccag ctgacgccgt cgcgctcccg
    38941 ccaggcgaag cctgcggacg tcaccagaac caccccccgg gcaggaggag gttccggaac
    39001 acgtcgagca cggtgccgaa ggtcgcctcg aagaacccgg gtacagcgat catgaccagg
    39061 aacaagccga tgaagacgaa gttgccgaag cgatcgagct ctgcccactt ccggtaggtc
    39121 gcccgcggaa ggaaccctcc gaggacacgc gacccgtcca gcggcgggat cgggatcagg
    39181 ttgagcgtca tcaggatgac gttgagcacg aagagcatcc agaacatctc gagcaggaac
    39241 aggctccacg tgtacgtcag ccagatgagg ccagtagcga cgaggaccag cgcgaagttg
    39301 gtgagcggtc ctgccgcccc gacccaggcc atgccgcgct gcccgtcctt gaagaagcgc
    39361 gggtccaccg gtacgggctt cgcccagccg aagaagaagc tgccgccgga gccgaggaac
    39421 gtgacggcga gcatgacggt cccccacagg tccaggtgcc tgatgggatt gagggtgagg
    39481 cggccgtgtg ccttggccgt gggatcgccg agccggtacg cgacccagcc gtgcgccagc
    39541 tcgtgcagca ccaggctcac gaggagaaga ggcgagacga cgaggaactg gaggaagagg
    39601 cccgagtcgt tcacgcgctc tcacgcatcc tggcggcggc cgtcagttcc tcttccctgc
    39661 cgccgaccac gccgttgagc ttgaggtgca ggacgctgcg gagcacctcg cccagtcgtg
    39721 gtccggagct gaaccccagc gcgaggaggt cggcgccgcc gatgtccagg cgcacgtgcc
    39781 gggagacgtc gaggaagtgc gccagccggt cggcggcgat gcccgactcg tcgagcgtca
    39841 tcgccgccag gaccgcctcc aggggctcgc cggcggccag gtcgtagagc tcggcctctc
    39901 cgggctgccg cgcgatccgg tcgaggagcc ggccggcgac cacgagcgca cgctccagga
    39961 cggaggcgtc ctgggcgcgg acgcgcatgc gcaccgccca ggcggcgatc tcctccgggt
    40021 cgaggtcgcg cagcagccac accaggcgga gccgccagcg cggcgtctcc cgctcgagcg
    40081 cgtgcgccgc gcgcagctcg tcggcgcggc gcacgagccg gcgcgtggcc ccgtccacac
    40141 gcagccgccc gtggatcgcc ggccacacgc cgagctcctc ggcgcggcgc agggcgaagt
    40201 cgaccttctg ctcgtccagg aggctcacca gctcgtcgcg gagccgcgcc gacgagaggt
    40261 cgccgacgag gtccatggcg cagcaggcgc gcgcgaggtt cagcgtgtgc ccgtccatgc
    40321 gcagctcgta gcggctctcg tagcggatgg cgcgcaggat ccgcgtggga tcctcgatga
    40381 agctgaggtt gtgcagcacg acgatgcggc cggccagcag gtcctcgcgg ccgccgaagt
    40441 agtcgaagag gtcgccgaag gtctccggct tgagcgacac cgccatggcg ttgatggtga
    40501 agtcgcgccg ggcgaggtcg ctgcggatgc cggcgtgctc caccttgggc agcgcgccgg
    40561 ggtaggcgta ggactccgcc cgcgtggagg cgacgtccac gcgcagcctg tccgtcccgt
    40621 cgtcggcgac gacgaccgcg gtgccgaact tctggtgcgg ccgcacgtgc cccttgagcc
    40681 tcgccgccag ctcggtcgcg aacgcgatcc cgtcgccctc gaccgccagg tcgacgtcga
    40741 acccgggctc gcccagcagc aggtcgcgga cggcgccgcc gacgaggtag cagccgcgca
    40801 cgccggcggc cacggcctgg acgtgacgga acagctcgtc caggcccagg tctcccaggg
    40861 gtccggcgag gttcgccgtc accggcgccg ccggctcgcg cggcggcgcc ccggcgaccg
    40921 agcggcgcgt gaccacgccg aggaggtcgc cgagcgcgac gccctcgtcc tccgaccgcg
    40981 cggactcgcc ggccacgacg ggcagccagc cgacctcgtc ctgcgcgagc ctcagcgcga
    41041 cccgctcgag cggcgtctgc gccccgacca gcggcacgct ggtcgtcatg accgccttga
    41101 ccggcgcgtg cccgagcccg tgcgccgacg cgcgctccag gtcggcgagg gcgacgcgcc
    41161 cggccagcag cccgtcgcgg acgacgccga cgccgccgag gccgtctcgc cggcagagga
    41221 gggcggcgtc atcgacggcg gcgacgtcgg cgaggacgac cgggccgggc tccagggcgt
    41281 cgagggcgac gcgcgtggga gccaccgccc ccggcgccgc ggccgccacg gccgccgcca
    41341 cctcgtcgag cgagcggtcc ttgacgacgg ccgaggcggc ggcggtgtgc ccgccgccgc
    41401 ccacggcgcg gagcgcctcg gccacgtcga ccctgccgcc gcggctgcgc gccgtcacga
    41461 acacgcggcc ctccatctcc acgagcagga agtacgcgtc gcagccggtc aggtccatga
    41521 cccggtgggc gacgacgctc acgccttcca cgtagaggtc ctggcgcaac gacgagagca
    41581 gcaccgacgc ctcggcgatg ggcagctcgt gcgccgtctg cagcgccgcc gccagggtga
    41641 gccgctgcgc cggcgtcagc gcgttggtca gccacttctc gagcagttcc gtgtccgcgc
    41701 cggcgcgcat gcagtaggcc agcgcctcgg cgtcgcgcgt cgtggtggtc gcgaaggtga
    41761 gagaccccgt gtcctcgtgg atgcccaggg cgaacacggt cgcctcgaac ggcgtcaccg
    41821 ggatcccccg ctcggcgatg atgcgcacga gcagcgtgac cagcgagccg tcgccggaac
    41881 tgaccagggc gtccgggtcg aggtagccgg gcaggtcgtc ggggtgggcg tggtggtcga
    41941 aggcgaccac ctgcacgccc gcgcggccgc agaggtcgcc gaggtcgccg agccggttgg
    42001 cgtgcacggt gtcgacgagg atcaagcggc ggacgctgtc ccgatccacg tccgcagggt
    42061 cgaccacggg gatggcgtcg gcgtacagcg cctggaactc gcgcacgttg cggttgaccg
    42121 cgccgccgag gctgatctgc gcctccgggt acagcttgtg cgcggcgacc atggcggcga
    42181 acgcgtcgaa gtcggtgttg ctgtgggtgg cgatgagttc gggcgtacgc atgggctgag
    42241 tatacgcggg cgccggcgag cgccccttca ggccttgagc agccagccga gctcgtgacc
    42301 gaacgccgcc tccatcgcat ccctgagggg agcgttgaca gggctcgcga ggccgggatc
    42361 ggcggcgagg atcccggccg ccagctcgcg cgcccggagc agcgcggagc ggtcgcgggc
    42421 gaggcgggcg agcttgaggt cgggcacgcc cgcctggcgc gcgcccagca cctgcccctc
    42481 cccgcggatc tcgaggtccc tgtcggccag ctcgaaaccg tcgttcgtct ccagcagcgc
    42541 ctcgagccgc tgctgggagc tgcccgtctc cgcctcggcg aacagcaggc agtacgactt
    42601 ctcgctgccg cgcccgacgc gtccgcgcag ctggtgcagc tgggcgaggc cgaagcgctc
    42661 cgcgccctcc acgatcatca ccgtggcgtt gggcacgtcg atgcccacct cgatcaggct
    42721 cgtggccacc agcacgtcca gatcgccggt cttgaacgcg cgcatgacct cgtcgcggtc
    42781 ggcggacctc agctggccgt gcgccaccgc gacgcgcgcg tcgcggaacg gcccggcctg
    42841 cagccgctcc gcctccgcca cggcggtggc ggcggagatg gactccgact cgtcgatggc
    42901 cgggcagacg acgtagacct gcctgccccg ggcgatctgc ttgcgcacga actcgtagcc
    42961 ggtctcgcgc tgcgtctcgt cgacgagccg cgtgaccacc ggctgccggc ccgaggggac
    43021 gccggcgatg gtggtcacgt ccaggtcgcc gaacacggtc agggcgagcg tccgcgggat
    43081 gggcgtcgcc gtcatgtgca gcacgtgcgg ggcgacgccg tcccgctcgg ctcggcgggc
    43141 gagcgcgtcg cgctgctcca cgccgaagcg gtgctgctcg tcgacgacca gcagcgcgag
    43201 cccggcgaag cgcacgtcct cctgcaggag ggcgtgcgtg ccgatcgcga tgcgggcctc
    43261 gcccgcggcg aggcgggcga gcgccgcccg ccgctccgcc gcgggcagcg acgccgtcac
    43321 cagctccgcc ggcgcgaggg cgccggccag ccgcgccgcg gtctccgcgt gctgcgcggc
    43381 gagcgtctcc gtcggggcga ggagcacgcc ctggaggccg gcctccgcgg cgcgcagcag
    43441 gcagtagagg gcgacgaccg tcttgcccga gcccacgtcg ccctgcagga ggcgccgcat
    43501 cgggacgtcc cggcgcaggt cgccctccag ctcgtccagc gccccgagct ggtgcgcggt
    43561 gagctcgaac ggcagcccct cgaggaacgt ccggctgagc tcaccgggct cggggagcgc
    43621 cggcgcgtgg acgcgctgct gctgctccgc cttgtgccgc agcagcccga cctgcatcag
    43681 cagcagctcc tcgagcacga gacgctcgcg tgcggcgccg gcctccgcga gcgagcgcgg
    43741 aaggtgcacg gccgtgaccg cgtcggcgcg gccgggcaga ccctcgcgca cgagcagctc
    43801 cgccggcagg gggtcgggca ggcggcgcat gaccggggcc acggcgtgga ccagcgcgcg
    43861 cagcagcttg gccgacacct gctcgctggc cgggtacacc ggcaccacgc cctcggtgtg
    43921 cacgccctcc cccacttcct cgtcgaggat ctcgtgcccc ttgaccacga acgactggcg
    43981 cccgccctgc ggccggaagg tcccgcgcag gctcagccgc atgccctcct gcaggatccg
    44041 cgtcaggtac ttctggttga accagaccgc gtccacggcg ccggtgtcgt cgcgcagcag
    44101 gaccctgacg atgtcgaccc gccggcgcgc cgtgcgctcg gcgcgcacgc gcacgacctc
    44161 gccgcggatg gtcgcctctt cccctaccct gaggtcgcgg atggccttgc ggtcgcggaa
    44221 gtcctcgtag cgccgcggga agtggacgag caggtcgccg acggtgtgca ggccgaaccc
    44281 ggcgaggcgg cgcgccacgg ccggcgtgac ctggtcgagg ccggccagcg ggagcgagag
    44341 gcttgagggg tcgaaggaga ggcgggtgag cggcggccgt tcgtcgtacc gaccgccgcc
    44401 gaagcggagc ggaagtggct gcccggtcac tccacgatca tggcgaacgc ggccgtgccc
    44461 ggcccgatgt gcgtgcccac gacggctccg acgtggccca ggaaggtgaa gtgcgcgttc
    44521 ggccgcacgc gctcgatcat ctcccggatg agcggcggct cctcgtcgtt gaccgcgtcg
    44581 atgagacaga agtagagctc gtcctccggc cggctgcgct cctcgacgaa gcgctgcatc
    44641 gccgccagcg ccttcttctg cccgcgtacc ttggcgtagc cggtgagcgt gccgtcgacg
    44701 gcggcgatga ggggcttgat gtccagcacg ccgccgacca gcgaggcggc gcggccgatg
    44761 cggccgccgc gacgcaggta ctcgagggtc gcggcgtgga cgagcagctc cgagtggtcg
    44821 cggaagtggc gcacgtaagc gtgcgcatcg tccagcgtgc agcctgccgc caggcggacg
    44881 cgcaggcgct cggcgcacat cgagagcgca gtcgtgaccg tgcgcaggtc gtagacgtac
    44941 acgtggtcga cctgctcggc cgcctgctcc gccgcccgca ccgtgccgct catctcgccg
    45001 gagaggtgca gggagaacac gtgctcgtac tgccgcctcg cctcctcgta gaccgcgacg
    45061 aactccccgg ccgtgggctg cgacgtggtc ggaaggacct ccgcctgctc gagcctggcg
    45121 aagaactcct gcgaggtcat gtcgacgccg tcgcggtaca tctggtcgcc gaagtgcacc
    45181 ttcagcggga ccatgtacag gccgggctgc tgcagccagc cgctcggggc gtcgcaggtg
    45241 gaatcgagga cgatggccgt gttgtcgagc gtgagaaggt cagtcatgtg tcccccctgc
    45301 ggcgcgtagt caacgttgcc cactgtcgac gcccttgcgg cgcgtccgac gatggtgtgc
    45361 ctgcggcgat cactgtcgac gcccctgcgg cgcgtccgac gatgatgtgc ctgcggcgat
    45421 cattcggcgc tcgccaggat cgggtagagc ggctgcccgc cttcgtggaa actgatctcg
    45481 gcgtccgggg cgacgcgggc ggcgacctcc tcgagctgct cacgcgtcgt gcccgacccg
    45541 ttgagcgccg tgagcacggt gacgtactcg gcgcctccgc gggcgaactc ctcgagcacg
    45601 ccgccgaacg cactctcgac gtcgtcggcg gccgccacca gcgtgccgtc gacgatgccc
    45661 atggtctggc ccttcttgac ctggaggccg tcgagctcgg agtcgcggac cgcgtgcgtg
    45721 acctcggcgt agcgcacgtc ggcgatcgca tcctgcatca aacgggcgtt gccgacggcg
    45781 tcctgcgccg ggtcgaaggc gatcatggcg gcgaggccgc tggggatgga ccgcgacggg
    45841 accacggcga tgtggagcga gctcatgccg gccgcctgct ccgccgtgag gatgacgttg
    45901 ccgttgttcg gcaggatcac gacctcctgg acgccgagcg cctcgaccgc ctcgagcagc
    45961 tgggcggcgc tcgggttcat ggactgtccc ccgtcgacga tggcgtggca gccgagctcg
    46021 cggaagagct gccggttgcc ggcgccggcc acgacggcga cgaccacggc gccaccctcg
    46081 ccgacctccg gggccggggt ggccagacgc tcgtcgcgtt cgcgcgtctg cgcgtgcatg
    46141 tcgttgatct cgacgtcccc gatcgcgccg tgcttgagcg cttcgctgag cacggcgccg
    46201 gggtcgttga cgtggacgtg caccttgacg gtgccctcgt cgcccaccac gagcgcgctg
    46261 tcgccgagcg gcaggaggaa cgcctcgaac gcctcacggt cgatgctttc gccggtgacg
    46321 agaaaactcg tgcagtagcg gaactcggag agctcgctcg gctcctcgac ggtgacggcg
    46381 tgcggccgcg caggccggac cgcaccgatg aacgcctcgc ccgccgcccg cgccgccacg
    46441 cgcacacccg cgatcgcctt accgccgcgc atgaggtcct cgatgcccgc ggccaggccg
    46501 cggaagagca cgagcaggcc gtagccgccg gcgtcgacga cgccggcctt ctgcagggcc
    46561 ggcagctgcg cggtcgtgtt ctcgaccgcg atcctgccgg cctcctccac ggcggcgagc
    46621 aggtgctcga gcccgagact gtcggggacc ccctgcgccg cggtcgccat ctcgcggatg
    46681 acggtgagca tggtgccctc gaccggcgtc ttgacggccc tgtaggcggc gtgctggccc
    46741 tcgacgacgg cgcgcttgaa cacggcggtg tcgagcgagt tggcctggcc ccacacctcg
    46801 cagatgccgc gcacgatctg ggagaggatg acgccggagt tgccgcgcgc gcccatcagc
    46861 gagcccttgg tcacggccgc ggcgaccgac gggatgtccc tgacgtcgct ctgctcgagc
    46921 tcctcgacca ccgcgcgcac cgtgagcgcc atgttggtgc ccgtgtcgcc gtccggcacc
    46981 gggtacacgt tgaggtcgtt gatgaactgg cgctcggcct cgacggccgc gagcgcggat
    47041 tggatgacgc cgatggcggc ggtacggacg atcaatcctg gagatcctcg acgcgcacgt
    47101 tgatctcgcc caccggcacg ccgcccacgt gctcgacctg gtagcgcacc tgttcccgga
    47161 ggttggcggt gacctgggcg atgttcacgc cccgctccat caccacgtgc aggccgatgt
    47221 cgacgctgcc ctccccgacc gcgagctcga cgccctcggt gacggagccg cggaagaacc
    47281 gggcgatctt cttgaccgtc ggatcctgca tcccgacgac gccgtaggtg gcccgcgcgg
    47341 ccacgccggc gagcttggcg atgacctggt gggcgacggt gacgccgccg acgatgccgc
    47401 cggccgtgaa ggggatgcgc aggtcagact gggacatggc ccctcgtcac tccccggcgg
    47461 tgatcagctg gttgagctcc gccaccacgg tctccggatc gacctggtgg aggatggcgc
    47521 ccgcctcgat gctctcggac tgggcgccga tgcacaggca gcaggccatc ccgtgactct
    47581 cgaacacctc gcgcgccgcg ggatccgtcg agagggcctc gaagatcgac atgtccttgg
    47641 tgaaccgcat cgacgcgcct gcccccgttt gcgttccccg aacgggtgaa ggatactagc
    47701 gcgaccgttc agcaagcaag ccgaggaacc catgtccaag atctgcgtga cctgcggcaa
    47761 ggcgcccgca gtcggcaaca acgtgagcca ctcgcatcgc cacacgaagc gccgcttcga
    47821 gcccaatctc cagcgtgtcc gcatcctcgt cgacggtcat ccgcagaacg cctacgtctg
    47881 cacccgctgc ctgaagtcgg gccgcgtcag caaggccgtt tagggcagcg ccgcgccggc
    47941 gccgccgcgc ccgccgtccc cgagtggaac acgatgagcc ccgccccggc ggggctcttg
    48001 cgttgggcgt ccaggtcggt cacgctgcga gcagaacggc gtgaccgggc gcagagcccg
    48061 gtcgaccgcg gaaggagcag cgtgccccac atcaccgcct gggcgttcgt gttcgccgtc
    48121 ggcgtggcca tcgtggtcgt cgccgtcgtc gcgatcaggg accggttcca caagtaggcg
    48181 ggcgccgccg acgaccgcgg cgcgcggccg tcagggcagc gtcttcgcca ggttcgccat
    48241 ctcgagggcg gtggacgcgg cggaccagcc cttgttgccc gccttggtgc cggcgcgctc
    48301 cacggcctgc tcgatcgtgt cggtggtgag cacgccgaag atcaccggga cgccggtgtc
    48361 gaggctgatc ttggcgatgc ccttggccgc ctcgccggcc acgtagtcga agtgcggcgt
    48421 gccgccgcgg atcacggcgc cgagacagat gacggcgtcg tacttgccgc tctccgccag
    48481 cctcttggcc acgagcggca tctcgaacgc gcccgggacc caggcggcgt ccacgtcggc
    48541 gtcgtccacg tcgtggcggc gcaggcagtc cagggcgccc tcgtagagcc tcccggagat
    48601 cagctcgttg aaccggccca ccacgagccc gaacttcatc cccttgccgt tcagcttgcc
    48661 ttcgtaggtc gtcatcctgc ggctccttct cgccgtgctg gtccctagcc ttcgaggccc
    48721 tcgtcctgcc cgaacaggtc ttcgtgctcg agcatgtggc ccatcttgtc gcgcttggtg
    48781 cgcaggtagc acctgttgtc ctcctgcggc gcgaactcga tctgctcgcg gccggtgacg
    48841 cgcaggccgt agccctccag cgcgacgatc ttcttggggt tgttggtcat gatgcggatg
    48901 gtggacaggc cgaggtcggc gaggatctgc gccccggtgc cgtagtcgcg caggtccggg
    48961 gcgaacccga gctccacgtt ggcctcgacg gtgtcgtagc cctgttcctg cagcgcgtag
    49021 gccttgagct tgttgatcag gccgatgccc ctgccctcct ggcgcatgta caggaccacg
    49081 ccgagcccct ccgcctcgat gcgccgcatc gcctcggcca gctgctcccc gcagtcgcag
    49141 cgcagcgagc cgaaggtgtc gccggtgagg cactccgagt ggacgcgcac cagcacgtcc
    49201 ttcttgccgg ccacgtcgcc cttgacgatg gcgacgtggt ggctgccgtc gagcacggac
    49261 tcgtacccga tggccttgaa gtcgccgtac cgggtgggca tggagaccgt cgccgcccgg
    49321 cgcacgaggt gctcgttcct gcgccggtac ttgatgaggt cggccacggt gatgatcttg
    49381 agcccgtgcc tgcgcgcgta ggggatgagg tcgggcaccc gcgccatggt gccgtcgtcg
    49441 ttcatgatct cgcagatcac accggacggg ttcaggtggg cgagccgggc caggtcgacg
    49501 gccgcctccg tctgcccgga gcgctcgagc accccgcccg gccgggccat gagcgggaac
    49561 acgtggcccg gctgcacgag gtcgccggga cgggactcgg ggtcgatggc gacctggatg
    49621 gtgtgcgccc tgtcggcggc cgagatgccg gtcgtcacgc cttcccgcgc ctccaccgac
    49681 accgtgaagg cggtctggag gcgcgcctcg ttgtggctgg tcatcggcgg caggtcgagc
    49741 tcgccgcagc gctgcggcgt gagcgcgagg cagatgaggc cacggccctc tttcgccatg
    49801 aagttgatgt tctccgcggt ggcgaactgg gccgccatcg tgaggtcgcc ctcgttctcg
    49861 cggtcctcgt cgtcgcagac gatgaccatg cggccctcgc gcaggtcctc gagcgccgcg
    49921 tcgatgctcg caaacggtgc gtccggcgcc tcgtcccgcg cggcgccggc cgtctctccg
    49981 ttgtggtcca ccgtcatcag aaccccgctt ccgctagctt ctcccaggtg agcccggcgc
    50041 cgggcttgcg cccggcgacg aacgcgtgca cgtacttggc gatcaggtcg gcctcgaggt
    50101 tgacccgaga accggtcgtg aggcgtccca gcgtggtgac cgccgccgtg tgcgggatca
    50161 gggacacgcg cacgcggtcg ccggcgaccg cgacgaccgt gaggctcacg ccgtccacgg
    50221 cgatggagcc ctggtccacg gtcacgcggg tgaccgcctc cggcgcctcg agctccacga
    50281 ccagggcgtt gtcctcaggc gtcaccgccg tgaccgcgcc gacaccgtcg acgtggccgc
    50341 tcacgaggtg accgccgagg cgtgagcgca gggtcatcgc gcgctcgagg ttgaggcggt
    50401 ctcccggacg acgctgcgcg agcgtcgtgc gccggacggt ctcgggcatg gcgtcggcgg
    50461 tgaacccgcc cgcgcgcagc gacgtgaccg tgaggcagac gccgtcggtg aggatgctgt
    50521 cgccgacggc ggcatcgtcc gtgaccgtcg tcgcggcgat gtcgagcacg acgctgtcgg
    50581 ctccgggccg gatgccccgc agcgtgccga tctcctcgac gatgccggtg aacaccctag
    50641 ccctcccggc gcacgacggt gccgctgagc atgaggtcgt cgcacagcgg ccgccacgtg
    50701 acgtcctgca ggcgccaggc gccgtccacg gactcgacgg cgggcgccgc gaacaggtcg
    50761 ggcgcgccgc gcccgacgat gagcggcgcg acgaacagga gcatgcggtc gatgaggccg
    50821 gcggccagca gcccgcccgc cagcgtcgga ccgccctcga agaggacgtc gagcaggccg
    50881 agtgcggcga gaccttcgag cgcggcccgg agcccgccgt cgcccacctc gagcagctcg
    50941 gcgccgcgcg catcgagcag gcggcgcgcg gcctccgtgg cctcatccgc cacgacgacc
    51001 gtgcgcgtcc gccgcgcgga cgccagcagc ctggcgccgg gaggcagctc cccgtcacga
    51061 gtgatcacca cgcggaccgg gtcgcggcct tccgagagcc gtacggtcag ctcggggtcg
    51121 tcgcggcgca ctgtgccgac gccgaccatg acggcgtcga cgcgcgcgcg cagctcgtgc
    51181 acggcgcgac ggctgtccag acagctgatc caccgcgcgt cgccgccggc cgccgccacc
    51241 ttgccgtcga gcgtcaccgc ggccttgtag gtcaggagcg gcagccccgt ggcgtggtac
    51301 ttgtggaagg gcgcgttctg ctcgcgtgcc cgcgcccccc agcggtcctc ggccaggtcg
    51361 acctccaccc cgccctcccg cagcctcgcc agccc
//
LOCUS       DASUMX010000068        22060 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1024308, whole genome shotgun sequence.
ACCESSION   DASUMX010000068 DASUMX010000000
VERSION     DASUMX010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 22060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22060
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1024308"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..685)
                     /locus_tag="VFZ86_03110"
     CDS             complement(71..685)
                     /locus_tag="VFZ86_03110"
                     /inference="COORDINATES: protein motif:HMM:NF021561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2284 domain-containing protein"
                     /protein_id="HEX5641311.1"
                     /translation="MADAMHLQSLITAAEGFGADRAAPVPATAVVVDDRVNLKCRVPL
                     CSGYGHNLMCPPAVLPADATRDVLRKYQDALVVQLDIPVTQDCVDEQLGGKTYAEAKS
                     DAEQIDGLRESQNRFAELMTDLERAAFKLGYRYAAAFSGGECVLCDECVGQASGEPCR
                     HPFKARPSMEAVGIDVVATAEAAGLSVELPAADHPAWTGLLLID"
     gene            complement(678..2690)
                     /gene="uvrB"
                     /locus_tag="VFZ86_03115"
     CDS             complement(678..2690)
                     /gene="uvrB"
                     /locus_tag="VFZ86_03115"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEX5641312.1"
                     /translation="MTEFTIKADYDLTGDQPEAVEALSEGILRGDRYQTLLGVTGSGK
                     TFTMANVIQKVKKPTLVIAHNKTLAAQLCNEFREFLPDNAVEYFVSYYDYYQPEAYVA
                     AQDLYIEKDSSINDEIDRLRHAATTSLFLRRDVVIVASVSCIYGLGSPEEYLEKMVLL
                     QVGQTVERDEMLRKLVDIQYARNDFEPARGKFRVRGDSVEIWPASQDTAIRVQLWGDE
                     VEQIVSFDPVGGEVIEKPEHVAIYPATHFVTNDPAIERSLVEIRREMEERCAWFEENG
                     KILEAHRLRQRTQYDMEMLREMGYCSGIENYSRILLGKEPGATPNCLLDYFPDDFLCF
                     VDESHMSLPQIRAMSEGDKSRKQQLVDYGFRLPSALDNRPLRFDEFLDKVPQLVFVSA
                     TPGAFELANSVNVAEQIIRPTGLIDPEVQVRPTEGQIDDLMNEIRRRVEKDQRVLVTT
                     LTKRMAEDLTDYLVENGFKVRYLHSEVDTLERIQIVRDLRLGEFDVLVGINLLREGLD
                     LPEVTLVAILDADKEGFLRGQTSLIQTIGRAARNIDGTVIMYADKMTAAMEVAIGETN
                     RRRARQTAYNEAHGITPVSVVKGVSDISTMLSDAAAVPTKGRRAAKAVAKKVTMPRAD
                     LEKLLASLEAEMFEAADQLKFEYAAKLRDEIRELGKELARRGEAVG"
     gene            complement(2740..3339)
                     /locus_tag="VFZ86_03120"
     CDS             complement(2740..3339)
                     /locus_tag="VFZ86_03120"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX5641313.1"
                     /translation="MRRALIGLTLVAVALVAVLGAARWVAPLFASGAPSWYTKTVYPL
                     EHVDAIRDGARRYDLEPSLVAAVVYAESRFDERARSSQGAVGLMQILPETAEQIARES
                     GGVSFTAADLEDPRVNVRYGCYYLRQALDAFDGDVRAAVASYNAGMGAVSQWRAEAAA
                     DGHELRPRDLPYAETRAYVKKVLQARRVYRETYGDRLQP"
     gene            complement(3336..3992)
                     /gene="coaE"
                     /locus_tag="VFZ86_03125"
     CDS             complement(3336..3992)
                     /gene="coaE"
                     /locus_tag="VFZ86_03125"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEX5641314.1"
                     /translation="MSTPRNHIPLLGLTGGIGSGKSTALAYLRELGAATISSDDIVHG
                     LYSNGDVVDRIREHFGDAVVDGAGGISRPALAGIVFSDEAELRWLEDLLHPFVRRAVD
                     DWIQERKKARPRPALIVVEVPLLFEAGFDRRFDFIMLITAPDDLRRRRLAAKLTDSEF
                     LRRRAQQMPEEEKIRRSDFVFDNTGPRKALREFVGQTAASILAGERPAVGDGAGESTS
                     "
     gene            complement(4058..5497)
                     /gene="rpsA"
                     /locus_tag="VFZ86_03130"
     CDS             complement(4058..5497)
                     /gene="rpsA"
                     /locus_tag="VFZ86_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002563956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEX5641315.1"
                     /translation="MADKPIDTQLLAANDPRIVEGSEGYLIEVDGEIVPNYDMTLTNF
                     SEGDIVTGKVVRIDKDEVLVDIGYKSEGVIPVNELSIRKNVNTSEEVALDEEIDALVM
                     TKEDADGRLILSKKRAKFEKAWKRIEGAFESGEPVTGTVIEVVKGGLILDLGVRGFLP
                     ASLVDIRRVQDLQEYVGQELNCRVIEMNRNRNNVVLSRRAVLEEERKEVRQQIIDELN
                     VGDIVEGTISNIVDFGAFVDLDGIDGLIHISELSWTHINHPSEVLQVNQKVKVKVLDI
                     DRDRQRISLGLKQTQEDPWQKIVARHKVGDVVDGRVTKIVAFGAFVEIYEGIEGLVHI
                     SELANRHVERPDEVVSVGQQVSVKIIEVDSERRRLSLSIKRVDGDNVKPLRPLAPEPG
                     DEEEAEETADAVEAEADTEAETEAEVEAEAAEAPDETEAAEESEEVAEAPEETVVEEP
                     EPELGLSDDVFPAGAAADDEAPAEESADA"
     gene            complement(5660..8275)
                     /gene="polA"
                     /locus_tag="VFZ86_03135"
     CDS             complement(5660..8275)
                     /gene="polA"
                     /locus_tag="VFZ86_03135"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEX5641316.1"
                     /translation="MADPHLYDRLFLLDGSSLAFRAFFALPESISTQAGQPTNALFGL
                     SQMLLKIVSEYRPSCIVCAWDAREKTFRDGLYDQYKAQRPSMPDALSAQWSLLPELME
                     GFGIANLVKPGYEADDILGTLAEEAKRQGIGSIVVTGDRDAMQVVDEDIWVMSTGRGV
                     TDVKIYTPAAVLERFGVTPAQIPDYIGLKGDSSDNIPGVPGVGEKTAAQLLQQFGSID
                     ELYRRLDEIKSEKRRALLAEHEDDARLSLQLATMVLDVPLEHDLVELVTSCGYRLPVE
                     TVDAVFERFEFANLRRRLREVSGEAAAAPAAPVSVAGGLVRLAAEPDVGPVAALLEKG
                     EAALAFEPRGDGDGLTVAAHAGGEACLVAPADAGRAGSLWLLAGHAVVHDAKAQPGFV
                     HAPQAPAFDTAVAAYLLAPERPEKERTLFALAGFDDGARLVEGPEAEAAAATRATLTW
                     RVAEAQRPRLVELGLERLFREIELPLVRVLARMEAAGVKMDPYRLGEITARVRDRIDE
                     LGDTITRLAGGPFTIGSPQQLAEVLFTRLGLEPARKGKTGYSTDARVLRGLRDQHPIV
                     PAVEEWRELTKLLNTYLEPLPARLDPVTGRLHTTFNQLVAATGRLSSSSPNLQNIPVR
                     TDLGSEIRSCFVAEEGHKLLVADYSQIELRLMASLSAEPALLEAYHRGEDVHRVTAAA
                     VAGIPVEEVSKRQRERAKATNFGIMYGLSAFGLSEQVEIPVEEARAFIDAYFAKYPRV
                     REFRERVIAQATQDGYVTTLFGRRRAVPELRSSNFRNRSLGERLAVNTILQGTAADII
                     KVAMVKVSRELERRGLASRLVLQVHDELVLECDEGEIDACRRLLRDAMCGALDLDPAL
                     EVDIGVGDDWVAAKA"
     gene            complement(8337..9095)
                     /locus_tag="VFZ86_03140"
     CDS             complement(8337..9095)
                     /locus_tag="VFZ86_03140"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5641317.1"
                     /translation="MTDRGVSTEVRGEICVIHCGGVTGGRLAADLPDEVDRCLDTGAS
                     AVVVDLDPWKPIDDLGLSALRAAAEAFESAGGELVVAVEEPDAREALAGAGLMRAIPS
                     APGPPGGDAEAPLRAPEHPRWEHEFTFPATTRQLRAARRRVAAFAEVAGLDGPDLFEL
                     SVAVAEALANAVVHGSPRGADDDVRVRFFCYEDEVAVEVVDSGDGMSATPICEPAAAA
                     TSGRGIHFMRALADAVQYTCGPMGTRVLLVKQRS"
     gene            complement(9101..9400)
                     /locus_tag="VFZ86_03145"
     CDS             complement(9101..9400)
                     /locus_tag="VFZ86_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641318.1"
                     /translation="MSETRNPRQPAPGDVQQWRADFDELELRMDALRQAFTEGVRSFT
                     MTDEIVERIHARFDDVGEQVHAMHCTYPFGGWPGQEDCRFEPPVPYESDARGDQG"
     gene            9582..10013
                     /locus_tag="VFZ86_03150"
     CDS             9582..10013
                     /locus_tag="VFZ86_03150"
                     /inference="COORDINATES: protein motif:HMM:NF015444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF296 domain-containing protein"
                     /protein_id="HEX5641319.1"
                     /translation="MGAREFARGRRVVARLPHGGDLLEEIAAVADAHEMQAAELRAIG
                     ALQTARLSFYDQATHEYGEFAVDAPVELVGLLGNVSRRDGATAVHAHATLAGHDGACA
                     GGHVAPGCVIFACELILQELVGEPLERVHDEVTGLPLWRGL"
     gene            complement(10084..10172)
                     /locus_tag="VFZ86_03155"
     tRNA            complement(10084..10172)
                     /locus_tag="VFZ86_03155"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10136..10138),aa:Leu,seq:gag)
     gene            10442..11377
                     /locus_tag="VFZ86_03160"
     CDS             10442..11377
                     /locus_tag="VFZ86_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016699852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX5641320.1"
                     /translation="MPARILVGSCSWTDPTLIACGRFYPPGVTSAEARLRHYAGQFDI
                     VEVDATYYAPPSLRNSELWVARTADDFVFNVKAFAWLTHHAAAPDRLPDWLRNRLPDE
                     VRGKRSVYARDVGERAMELVWQLHREALTPLAEAGKLGAVLFQFPPWFVRSRENRDYL
                     RSLPERLPGWPLAVEFRGGGWLEEDSAANTLALLEEAGLVYVAVDEPQGFPNSTPPLS
                     AATAPLGMVRLHGRNAGTWESRTGAASDRFKYLYSDDELEEWVPKVRELAAAADTVHV
                     LFNNNYEDWGMQNARRMAHLLGVERRPPQTQLELG"
     gene            complement(11400..12587)
                     /locus_tag="VFZ86_03165"
     CDS             complement(11400..12587)
                     /locus_tag="VFZ86_03165"
                     /inference="COORDINATES: protein motif:HMM:NF027964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HEX5641321.1"
                     /translation="MKIAVVGAGAMGRWAVKELGLAPEVDAVVVGDFNEDQAREVAAS
                     HGGGKATGRFVDARDQESVQAAIAGCDALVNATQHFWNITVMHAAAAARVHYTDMGGL
                     FHVTKQQVELHEEFRQAGVTAVIAMGGAPGVTNILARYGAERLDVVEEAHALCGNVDD
                     TDWSGYDGWVVPYSLETLCDEFSVVAPEFIDGHWVMDIAGGEGVEDLDFGVPAGTLEA
                     HYTIHSEPFTFWHAWKDQGLRSATFKLSLPAEFTGQMRFLNAIGMTRTDEVEVKGVRV
                     RPRDVLLKVVSEIPPPADVVLDDVDYLLGVVRGLKDGRRVEWRVRATVPAHKEHGAGG
                     GDVDTGIPPAIVARMLARGDIAGPGVFVPEQVVPGEAFVAEFTRWGVTVDAQMRELLA
                     APG"
     gene            12708..14072
                     /locus_tag="VFZ86_03170"
     CDS             12708..14072
                     /locus_tag="VFZ86_03170"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX5641322.1"
                     /translation="MTVARYGLSESEWQALEDEVLELFTALLRVDTTNGNETEAALVV
                     QAYLAENGIPGELDGELPDRKSLVARLDGARPGATLTMMGHLDVVPADAGEWSVPPFA
                     GVVKDGYVWGLGAVDMKNQVAAEAVALARLKRAGASFSGTVKYAATADEEVGEHCGVR
                     WLCEHRPEALRADYLVNEGMGGLWLPVDGHKVFLLAVGEKAFAQFRIRTRGRGGHGSV
                     PEKERSAVLDLARAVIALGVADPPAIVSGTTARFIDALVPDRELAARLKDPATARVAG
                     AELRRLDPVVAGLVEPLFGATLTPTVLAAGKAVNVIPTRAEACVDCRILPEMAQADVR
                     EYVTTVLDPLGIEWEFEWVDVTTPNASPASTPLSESIERVLRRDVPDAVLAPMTSSSF
                     TDSRWVREAFPDCVAYGFAPFLAESFHAMDGGRDHEPDERILVEDVVYQALFFERLAG
                     DLLA"
     gene            14069..15004
                     /locus_tag="VFZ86_03175"
     CDS             14069..15004
                     /locus_tag="VFZ86_03175"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEX5641323.1"
                     /translation="MSPAGEAAVMRDRFRGALLGLAAGDALGAPAEFLTADQVVEKYG
                     VITGMIGGGVYDVAPGEVTDATEMMLCLAESLAEHGAFEPEDIMARLGAWLETQPRHV
                     SLTVRAALISYRSGTHWDVASRRAYEILGGPTAGNGSLIRCAPVGLLYSGDAAERHGV
                     SLRESTLTHFDRLAGWSCAAFNDLISAALNGELAADIPAIASTFDEDDKRVAAVLRDT
                     PVAEAEEIVSSSFVLDTLQTALWTVLHGADFEHAVTVAVNMGNDATAAGAVTGALAGA
                     VYGESGIPARWLDELTVRDRVTAAADKLAALAGVG"
     gene            15120..15650
                     /locus_tag="VFZ86_03180"
     CDS             15120..15650
                     /locus_tag="VFZ86_03180"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX5641324.1"
                     /translation="MDERERRRAERLLVEEYGLLFEEMGGTRMEGRVLAWLLLADPPE
                     QSLTGIAQGLGVSKAAVSTAARSLLQSRAVERVSEPGHRGDFYRSIPGKLESVLRLDH
                     VTALGRLVDRSLELVADGDQTQSNYALLREMRAFIDFLEAEIPGLVSRWQRHRAANHT
                     LADGGDRDNDHTGGAT"
     gene            15647..18874
                     /locus_tag="VFZ86_03185"
     CDS             15647..18874
                     /locus_tag="VFZ86_03185"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX5641325.1"
                     /translation="MSWLTKISLRNRSIVGLAVLAVLLVGAFAITGLKQELIPDLTFP
                     YLTVVTVDQGSSPKDVERNITTPLEQAIKTSSGVKEFDSFSNDGMSIITIEYEFGTDM
                     DARQAEIQQAVSGVQQMLPATAMPPEVATLNFGSMPVVQLAVTSSLPPEQLATLLGTR
                     VVPRLQSIEGVQAVTLSGVQQMQLNIQLKPEQVAALGVTPTQIMTAVTQANVTTGAGS
                     VTSGSIVYPVTVSAKAETEKALEQLVLAPSSAASAAGAAAATGASAVPAEADAMAAAA
                     AGEAAADKYRQTVRLGDVADVEIAPAPLTAVTRTNGETSVGISVSKSSSGNTVDIANA
                     VADELPALTRELGGDTEITTVVDQSVFIKDSIRSLVREGLLGAVFAVVVIWVFLRSWR
                     STVIAGLSIPLSVIGALIILWSRGESLNMLTLGGLTIAIGRVIDDSIVVIENIYRHLQ
                     EGDSVRRAAYTGTREVAGAITASTLTTVAVFLPLGMVAGLASEFFRPFALTVTFALLC
                     SLLVALVVVPVASTWLLSKRQVGHRDERELTALQNAYLPALRFALGHKAITLIAAAAI
                     FVGTMLFTPLLKTNLFDSSEQPTMSITQTMEPGTSLDATMAAASKVEEVLKSTEGVEI
                     YQVTGGSTGSLFGAGGGTNASSSQATFAVTTDPDADKNAIMDEVRAGVAELENAGTIS
                     VTGDDSSSSMGGMSLMEVRISAGDPQVLKEANDIVLAEVETVDGLADVTSNLSEGRPS
                     ATIVVDQAKAAAAGVTPATLSQYTTLVLNGYPLGTVPTTAGTLPAQLMVGEIVVPPLP
                     GAVSQVLARLPVAGTSGMVPLGDIAEVVEVKAPVQVTHVDGARTASITATAVNNNIGA
                     ASTAVEDALKDVQLPEGATWELAGATEATNDVFRTLGIAMLVAILLVYIIMVATFRSL
                     LNPLILLVSIPFAAVGAVLALVITGTSLGMPSLVGLLMLIGIVVTNAIVLLDLVEQFR
                     RKGMDARTAVIEGGRRRLRPILMTAIATILALTPMALGIGGEGGFLSTPLAVVVIGGL
                     FTSTFLTLILVPVLYLAFDRLRPAGAYALDDEDYTTMAAEPAPAD"
     gene            19029..19541
                     /locus_tag="VFZ86_03190"
     CDS             19029..19541
                     /locus_tag="VFZ86_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX5641326.1"
                     /translation="MAKPAPKKRAAKASVATKPRTAAEMQRPVPRELTRAELIDELNR
                     DLAKEFQALVQYVQHAAAMTGPQYDAIKAELVVHSNEEHAHAISLADQIDFLGGVPGV
                     DVAEALISPDAKTMLEQDLDGELDAIARYRERIAQAEMLQEYGLRRALEDILIVEEEH
                     ARDLQDALDR"
     gene            complement(19570..20817)
                     /locus_tag="VFZ86_03195"
     CDS             complement(19570..20817)
                     /locus_tag="VFZ86_03195"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5641327.1"
                     /translation="MLARLRRYLDFPGQFWVLTLGIFIYVGAAALAFPFEAIYLRQYL
                     GTSMTMIGVVFGLVPLAVMPVQFWGGQLTDKLGRRVIIMVSVLVGVVWFVGFAFVTEL
                     WQVAALVALESAFGWPLFQTASNAMIADLLPQERRQEGFGVTRAAMNFGVVVGPVMAG
                     QALGFGVSFRVLFLSAAAGCLAMVVMMAVWIRESRPASATVRVRRVDERGRAGYRAVL
                     HDRVFIVFCLAAVLPVFCIGNFGSIYAVYITDFLGVPSRTWAYLLTLNALIVVVVQIP
                     LVTALRRRNRMLLLAASSALLAVGIGGSAFAGPVWSLVAFIAILSLGETLLSPVASAE
                     VAELAPEAVRGRYMGVWTVVWNGGAALGPAFGGWAMDTVGGREAFVALLVVGLAGAVW
                     FLALAPGWGRRRRAKAVEARATA"
     gene            20988..>22060
                     /locus_tag="VFZ86_03200"
     CDS             20988..>22060
                     /locus_tag="VFZ86_03200"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX5641328.1"
                     /translation="MYLSVQQAARRLGVSPVTIRRWTATGFLPCTRTAGGHRRIDDHD
                     IDDLAKAIGGSNHLAAQLAREREVDVLINTSTALAVKLDLTELLLEIAMRMTSLFQCH
                     LCAISEYDPEDDAVRTLAEYDNAGRQLLDASPYRLREFPLTRRVLAEQVTAVVNVDDP
                     HADPAEVAELRREGDRSLLMVPLVVQGRSLGLLEVVDQKVARQYSRQELRLAQAIAGQ
                     AAVAIQNAKLFAERRRSDEDVAYLREALSTLSAHVPRLGEGSRPGDVLTTVADAVCEA
                     LGAISCVASMGGESAGAFGAGRVAAPSGSDAARGANASLLVVRDPSGRTDLTLAVTLP
                     HPPGEGQEELLDFVAAVAVLVAG"
BASE COUNT         3178 a         8016 c         7669 g         3197 t
ORIGIN      
        1 ctcagggaga tgtccttgag gttgtgctcg cgcgcgccgg tgatgatgag gtcggaggtc
       61 atacttcccg tcaatcgatg agcaggagac ccgtccaggc ggggtggtcc gcggccggca
      121 gctcgacgct gaggccggcc gcctcggccg tcgcgacgac gtcgatgccc acggcctcca
      181 tggacggccg cgccttgaac gggtgccggc agggctcgcc cgacgcctgc cccacgcact
      241 cgtcgcagag cacgcactcg ccgcccgaga aggcggcggc gtagcggtag cccagcttga
      301 aggcggcgcg ctcgaggtcg gtcatgagct ccgcgaaccg gttctggctc tcgcggagcc
      361 cgtcgatctg ctcggcgtcc gacttggcct cggcgtacgt cttgccgccg agctgctcgt
      421 ccacgcagtc ctgcgtgacc gggatgtcga gctggaccac cagtgcgtcc tggtacttgc
      481 gcaggacgtc gcgggtcgcg tccgccggca ggacggccgg cgggcacatg aggttgtggc
      541 cgtagccgga acacagcggc acacggcact tgaggttgac gcggtcgtcg acgaccacgg
      601 ccgtcgccgg caccggcgcc gcgcggtcgg cgccgaagcc ctccgccgcc gtgatgagac
      661 tctgcaggtg catggcgtca gccaaccgct tctcctctcc gggcgagctc cttgccgagc
      721 tccctgatct cgtcgcgcag cttggcggcg tactcgaact tgagctggtc cgccgcctcg
      781 aacatctcgg cctcgaggct cgcgaggagc ttctcgaggt cggcgcgcgg catcgtgacc
      841 ttcttggcca cggccttggc ggcgcggcgg cccttggtgg ggaccgccgc cgcgtcgctg
      901 agcatcgtgg agatgtcgct gacgcctttg accacgctca ccggcgtgat gccgtgcgcc
      961 tcgttgtagg ccgtctgccg ggcgcggcgc cggttggtct cgccgatcgc cacctccatc
     1021 gccgccgtca tcttgtcggc gtacatgatc accgtgccgt cgatgttgcg cgcggcgcgg
     1081 ccgatggtct ggatgaggct cgtctgcccg cgcaggaacc cctccttgtc ggcgtcgagg
     1141 atggcgacca gcgtgacttc cggcaggtcg aggccctcgc gcaggaggtt gatcccgacg
     1201 agcacgtcga actccccgag ccgcaggtcg cgcacgatct ggatgcgctc cagcgtgtcg
     1261 acctcgctgt gcaggtagcg caccttgaag ccgttctcga cgaggtagtc cgtgaggtcc
     1321 tcggccatgc gcttggtcag cgtggtgacg aggacgcgct ggtccttctc cacgcgccgc
     1381 cggatctcgt tcatgaggtc gtcgatctgc ccctcggtgg gccgcacctg cacctcgggg
     1441 tcgatcagcc cggtaggccg gatgatctgc tccgccacgt tgacgctgtt ggcgagctcg
     1501 aacgcgcccg gggtggcgct cacgaagacg agctgcggca ccttgtcgag gaactcgtcg
     1561 aatcgcagcg gccggttgtc cagcgcgctc ggcaggcgga agccgtaatc caccagttgc
     1621 tgcttgcgcg acttgtcgcc ctcggacatg gcgcggatct gcggcagcga catgtgggac
     1681 tcgtcgacga agcagaggaa gtcgtcgggg aagtagtcga gcaggcagtt gggcgtggct
     1741 cccggctcct tgcccagcag gatgcgggag tagttctcga tgccgctgca gtagcccatc
     1801 tcgcgcagca tctccatgtc gtactgcgtg cgctggcgca ggcggtgcgc ctcgaggatc
     1861 ttgccgttct cctcgaacca ggcgcagcgc tcctccatct cacgacggat ctccacgagc
     1921 gagcgttcga tggccgggtc gttggtgacg aagtgggtgg ccggatagat ggccacgtgc
     1981 tccggcttct cgatgacctc gccgccgacg gggtcgaagc tgacgatctg ctccacctcg
     2041 tcgccccaga gctgcacgcg gatggcggtg tcctgtgagg cgggccagat ctcgacggag
     2101 tcgccgcgca cgcggaactt gccgcgcgcg ggctcgaagt cgttgcgcgc gtactggatg
     2161 tcgacgagct tgcgcagcat ctcgtcccgc tccacggtct ggccgacctg caggagcacc
     2221 atcttctcga ggtactcctc gggagagccc aggccgtaga tgcagctgac gctggcgacg
     2281 atcacgacgt cgcggcgcag gaacagcgac gtggtcgccg cgtggcgcag ccggtcgatc
     2341 tcgtcgttga tggacgaatc cttctcgatg tagaggtcct gcgccgccac gtaggcctcg
     2401 ggctggtagt agtcgtagta gctgacgaag tactcgaccg cgttgtcggg caggaactcg
     2461 cggaactcgt tgcagagttg cgccgccagc gtcttgttgt gggcgatgac cagcgtgggc
     2521 ttcttcacct tctggatgac gttggccatg gtgaaggtct tgcccgagcc ggtgaccccg
     2581 agaagcgtct ggtagcggtc gccgcggagg ataccctcgc tgagggcttc gacggcctcc
     2641 ggctggtcgc cggtgaggtc gtagtcggcc ttgatcgtga actccgtcat gcaccctcga
     2701 tgctaccaat acgaacacgt gttcgcaagg gcgtcccgcc tacggctgca ggcggtctcc
     2761 gtacgtctcc cggtataccc ggcgcgcctg gaggaccttc ttgacgtacg cccgagtctc
     2821 cgcgtacggg aggtcgcggg gacgcagctc gtgcccgtcg gcggccgctt cggcgcgcca
     2881 ctgcgagacg gcgcccatgc cggcgttgta cgaggccacg gcggcgcgga cgtcgccgtc
     2941 gaacgcgtcc agcgcctgcc gcaggtagta gcacccgtag cgcacgttca cccgagggtc
     3001 ctcgaggtcg gcggccgtga agctgacgcc tccggactcg cgggcgatct gctcggccgt
     3061 ctccgggagg atctgcatga ggcccacggc cccctgggac gagcgcgcgc gctcgtcgaa
     3121 acggctctcg gcgtagacga cggcggccac gagcgacggc tcgaggtcgt agcggcgcgc
     3181 gccgtcacgg atggcgtcga cgtgctccag cgggtagacc gtcttggtgt accagctcgg
     3241 cgcgccgctc gcgaacagcg gcgccaccca gcgggccgcg ccgagcaccg ccacgagagc
     3301 gaccgcgacg agggtgagcc cgatgagggc gcgcctcacg aggtgctctc gccggcgccg
     3361 tcccccacgg cgggtcgctc gccggcgagg atgctcgccg ccgtctgccc cacgaactcc
     3421 ctgagcgcct tgcgcggccc ggtgttgtcg aacacgaagt cgctgcgcct gatcttctcc
     3481 tcctccggca tctgctgtgc ccggcggcgt aggaactcgc tgtcggtgag cttggccgcg
     3541 aggcgcctgc ggcgcaggtc gtcgggcgcc gtgatcagca tgatgaagtc gaagcgccgg
     3601 tcgaagccgg cctcgaacag cagcgggacc tcgaccacga tcagcgccgg ccgcggccgc
     3661 gccttcttcc gctcctggat ccagtcgtcg accgctctgc gcacgaacgg gtggagcagg
     3721 tcctccagcc agcgcagctc ggcctcgtcc gagaagacga tgccggcgag cgccgggcgg
     3781 ctgatcccgc cggctccgtc cacgacggcg tcaccgaagt gctcgcggat gcggtcgacc
     3841 acgtcgccgt tcgagtagag gccgtgcacg atgtcgtcgc tcgagatggt ggccgcgccg
     3901 agctcgcgaa ggtacgcgag cgcggtcgac ttgcccgagc cgatgccgcc cgtcaggccg
     3961 agcagcggga tgtggtttct gggtgtgctc atgagcgtcc cgcgcaggac ggggcggcgg
     4021 aagcgcctga cgctcccgcc gccccgtcct cgcagcgtca ggcgtccgcc gactcctcgg
     4081 ccggagcctc gtcgtccgcg gccgcgccgg ccgggaagac gtcgtcgctc aggccgagct
     4141 cgggctcggg ctcctcgacc acggtctcct cgggggcctc ggcgacctct tcggactctt
     4201 ccgccgcctc ggtctcgtcg ggagcctcgg cggcctccgc ttcgacctcg gcctcggtct
     4261 cggcctccgt gtcggcctcg gcctcgacgg cgtcggccgt ctcctcggcc tcctcctcgt
     4321 cacccggctc gggggcgagc gggcgcagcg gcttcacgtt gtcgccgtcc acgcgcttga
     4381 tgctgaggct gagacgacgc cgctcggagt cgacctcgat gatcttgacg ctgacctgct
     4441 ggccgacgga cacgacctcg tcgggacgct cgacgtggcg gttggccagc tcgctgatgt
     4501 gcaccagacc ctcgatgccc tcgtagatct cgacgaaggc gccgaaggcg acgatcttgg
     4561 tgacgcggcc gtcgaccacg tcgccgacct tgtggcgggc gacgatcttc tgccacgggt
     4621 cttcctgggt ctgcttgagg ccgaggctga tgcgctggcg gtcgcggtcg atatcgagca
     4681 ccttgacctt gaccttctgg ttgacctgca gcacctcgga cgggtggttg atgtgcgtcc
     4741 agctgagctc gctgatgtgg atgaggccgt cgatgccgtc gaggtcgacg aacgcgccga
     4801 agtcgacgat gttgctgatg gtgccctcga cgatgtcgcc cacgttgagc tcgtcgatga
     4861 tctgctggcg gacctccttg cgctcctcct cgaggacggc gcggcggctg aggaccacgt
     4921 tgttgcggtt gcggttcatc tcgatgacgc ggcagttgag ctcctggccg acgtactcct
     4981 ggagatcctg cacgcggcgg atgtcgacca gcgaggccgg caggaacccg cgcacgccga
     5041 ggtcgaggat gagaccgccc ttgacgacct cgatgacggt gccggtgacc ggctcgccgc
     5101 tctcgaaggc gccctcgatg cgcttccacg ccttctcgaa cttggcgcgc ttcttgctca
     5161 ggatgaggcg cccgtcggcg tcttccttgg tcatcacgag ggcgtcgatc tcttcgtcga
     5221 gcgcgacttc ctcggaggtg ttgacgttct tgcggatgct cagctcgttg accgggatga
     5281 cgccctcgga cttgtagccg atgtcgacga ggacctcgtc cttgtcgatg cggaccacct
     5341 tgccggtgac gatgtccccc tcgctgaagt tggtcagcgt catgtcgtag ttggggacga
     5401 tctctccgtc gacctcgatg aggtagcctt cgctcccctc gacgatcctg gggtcgttcg
     5461 ctgccagaag ctgtgtgtcg ataggtttgt ctgccatgtg gtgtcgttgc ctcccggtca
     5521 cgtatgcggt ccgtcgttta cagatgggaa aggctcgcgg accgcagcga gccgaaacca
     5581 gcggagtata gcattcctgg agcggtgaat cgaacctcgc ggggcgccgc gagggtgatg
     5641 cggaccggcg gcgcggccgt caggccttcg ccgccaccca gtcgtcgccg acgccgatgt
     5701 cgacctcgag cgcggggtcg aggtcgaggg cgccgcacat ggcgtcgcgg agcagtcgcc
     5761 ggcacgcgtc gatctcgccc tcgtcgcact cgaggacgag ctcgtcgtgc acctgcagca
     5821 ccagccgcga cgcgaggccc cggcgctcca gctcgcggct caccttgacc atggcgacct
     5881 tgatgatgtc cgccgccgtc ccctgcagga tggtgttgac ggccaggcgc tcgcccagcg
     5941 agcggttgcg gaagttggac gagcgcagct cgggcacggc ccggcgccgg ccgaacagcg
     6001 tcgtgacgta gccgtcctgc gtcgcctggg cgatcacgcg ctcccggaac tccctgacgc
     6061 gcgggtactt ggcgaagtag gcgtcgatga aggcgcgcgc ctcctcgacc ggtatctcga
     6121 cctgctccga gagcccgaac gccgagagcc cgtacatgat gccgaagttg gtcgccttgg
     6181 cgcgctcgcg ctgccgcttg ctgacctctt cgaccgggat gccggcgacc gcggcggcgg
     6241 tgacgcggtg cacgtcctcg ccgcgatggt acgcctccag cagcgccggc tccgcggaga
     6301 gcgacgccat gagacggagc tcgatctggg agtagtcggc gaccaggagc ttgtgcccct
     6361 cctcggccac gaagcaggaa cgtatctcgg agcccaggtc ggtgcgcacg gggatgttct
     6421 gcaggttggg gctcgagctc gagagccggc cggtggcggc caccagctgg ttgaaggtgg
     6481 tgtggagccg gccggtgacg ggatcgaggc gggccgggag cggctcgagg taggtgttga
     6541 gcagcttggt gagctcccgc cactcctcga ccgcgggcac gatggggtgc tggtcgcgga
     6601 ggccgcgcag gacgcgcgcg tcggtcgagt accccgtctt gcccttgcgc gccggctcga
     6661 ggccgaggcg cgtgaacagg acctcggcca gctgctgcgg cgagccgatg gtgaacggcc
     6721 cgccggcgag ccgggtgatc gtgtcgccga gctcgtcgat gcggtcgcgc acgcgcgccg
     6781 tgatctcgcc gagccggtag gggtccatct tcacgccggc cgcctccatc cgggcgagca
     6841 cgcgcaccag cggcagctcg atctcccgga acagccgctc gagcccgagc tcgacgagcc
     6901 gcggccgctg ggcctcggcc acgcgccagg tgagcgtggc gcgcgtggcc gcggccgcct
     6961 cggcctccgg cccttccacc aggcgcgcgc cgtcgtcgaa gccggcgagc gcgaagagcg
     7021 tgcgctcctt ctccggccgc tccggcgcga gcaggtacgc ggcgaccgcg gtgtcgaagg
     7081 ccggcgcctg cggcgcgtgc acgaagccgg gctgcgcctt ggcgtcgtgc accacggcgt
     7141 ggcccgcgag cagccagagc gagccggcgc gaccggcgtc ggccggcgcc accaggcacg
     7201 cctcgccgcc ggcgtgggcg gcgacggtga gaccgtcccc gtcgccgcgc ggctcgaagg
     7261 cgagcgcggc ctcccccttc tccagcaggg cggccacggg gccgacgtcc ggctccgccg
     7321 cgagccgcac gagaccgccg gcgacgctca cgggcgcggc cggcgcggcc gccgcctcgc
     7381 cggagacctc acgcagacgc cggcgcaggt tggcgaactc gaagcgctcg aagacggcgt
     7441 ccacggtctc gaccggcagc cggtagccgc agctggtgac gagctcgacg aggtcgtgct
     7501 cgagcgggac gtccagcacc atcgtcgcga gctgcagcga gagacgcgcg tcgtcctcgt
     7561 gctccgcgag gagcgcgcgc cgcttctcgc tcttgatctc gtccagccgc cggtacagct
     7621 cgtcgatgga gccgaactgc tgcagcagct gcgccgcggt cttctcgccg acaccgggca
     7681 cgccggggat gttgtccgag ctgtcgccct tgagcccgat gtagtcgggg atctgcgccg
     7741 gcgtgacgcc gaagcgctcc agcacggcgg cgggcgtgta gatcttgacg tcggtgacgc
     7801 cgcggccggt gctcatcacc cagatgtcct cgtcgaccac ctgcatcgcg tcgcggtcgc
     7861 cggtgacgac gatggacccg atgccctggc gcttggcctc ctccgccagc gtcccgagga
     7921 tgtcgtcggc ctcgtacccg ggcttgacga ggttggcgat cccgaagccc tccatcagct
     7981 ccggcaggag actccactgg gccgagagcg cgtccggcat ggagggacgc tgcgccttgt
     8041 actggtcgta gagcccgtcc cggaaggtct tctcgcgggc gtcccaggcg cagacgatgc
     8101 acgagggccg gtactcggag acgatcttga ggagcatctg gctgaggccg aagagcgcgt
     8161 tggtgggctg cccggcctgg gtggagatgg actcgggcag cgcgaagaag gcgcggaacg
     8221 cgaggctgct gccgtcgagc aggaagaggc ggtcgtacag gtgagggtcg gccatgaggc
     8281 gattctacgt cacgccgccg gcgcgccgtc ggccggggtc tgcggcccgc cgcgactcac
     8341 gagcgctgct tgaccagcag cacgcgcgtg cccatgggac cgcacgtgta ctgcacggcg
     8401 tccgccagcg cgcgcatgaa gtggatgccc cgcccgctcg tcgccgccgc cgccggctcg
     8461 cagatcggcg tggcggacat gccgtcgccg ctgtccacca cctcgacggc gacctcgtcc
     8521 tcgtagcaga agaagcgcac ccgcacgtcg tcgtcggcgc cccgcggcga cccatggacg
     8581 accgcgttcg ccagcgcctc ggcgaccgcc acactcagct cgaacaggtc cgggccgtcg
     8641 aggccggcga cctccgcgaa cgcggccacc cgccggcgcg ccgcgcgcag ctggcgcgtg
     8701 gtcgcgggaa aggtgaactc gtgttcccag cgggggtgtt ccggcgcccg cagcggcgcc
     8761 tccgcatccc cgccgggagg gccgggagca gacggtatcg cgcgcatcag tccggcgccg
     8821 gcgagcgcct cgcgggcgtc cggctcctcg accgcgacga ccagctcgcc cccggcgctc
     8881 tcgaacgcct cggcggccgc ccgcagggcg gagaggccga ggtcgtcgat cggcttccag
     8941 gggtcgaggt cgaccacgac cgccgacgcg cccgtgtcga ggcagcggtc cacctcgtcc
     9001 ggcaggtcgg cggcgagccg gcccccggtc acgccgccgc agtggatgac gcagatctct
     9061 ccgcgcacct cggtactgac gccgcggtcc gtcacacgga tcagccctgg tccccgcggg
     9121 cgtcgctctc gtaagggacg ggcggctcga agcggcagtc ctcctggccc ggccagccgc
     9181 cgaacgggta ggtgcagtgc atggcgtgga cctgctcgcc cacgtcgtcg aagcgtgcgt
     9241 ggatgcgctc cacgatctcg tcggtcatgg tgaaggagcg cacgccctcg gtgaacgcct
     9301 ggcgcagcgc gtccatgcgc agctcgagct cgtcgaagtc ggcgcgccac tgctgcacat
     9361 cgccgggagc gggctggcgc gggttgcggg tctcgctcat atcgatcctc cgtgacgtgc
     9421 gatccgaacc gggccacatg acggcccgag acccacatac cctgcggtga ggccccgtac
     9481 acggggcggc gcccccggcg ccgggcgcgg tcgtcgcggt tgtacactcc ggccggaggg
     9541 acggcacacg gccggccccg gggagtcggg aggaagctgg catgggagca cgagagttcg
     9601 cgagaggacg ccgcgtcgtc gcccggctac cgcacggcgg cgacctgctg gaggagatcg
     9661 ccgcagtggc cgacgcccac gagatgcagg cggcggaact gcgcgccatc ggcgccctcc
     9721 agacggcccg cctctccttc tacgaccagg cgacgcacga gtacggcgag ttcgccgtcg
     9781 acgcgcccgt cgagctggtc ggcctgctcg ggaacgtgtc gcgccgcgac ggcgccaccg
     9841 ccgtgcacgc gcacgccacg ctggcgggac acgacggcgc ctgcgccggc ggccacgtcg
     9901 cgccgggctg cgtgatcttc gcctgcgagc tgatcctgca ggagctcgtc ggcgagcccc
     9961 tggagcgcgt gcacgacgag gtcaccgggc tgccgctctg gcgcggcctc tgagcgcccg
    10021 ctgcgccggc ccccggcgcg gcagagcacg cgcgatgagg aggcgtgagg cggaggaaga
    10081 aggtggtgtc ggaaggggga cttgaacccc catgaggtta taccctcact aggccctcaa
    10141 cctagcgcgt ctaccaattc cgccattccg acactgaggg agtgcgagta taggacccgc
    10201 gccggcaaag ttcaacgcgg gccggcggcg gcgcgggcga cctccggcgg tcatgacgat
    10261 gggcgggccc gtgttccgca gcccgcccat cggcggcgcc cccttcatct gcctccaaga
    10321 gcccgaggcc gtggtctgca gagaccgtgg cgccgactga gctttcgccc ggagtatagg
    10381 ccgggcgctc cccgtggtac aacgtttcta cgccacgcaa ccatctcgac ccggaggaac
    10441 gatgcccgca cgcatcctcg tcggcagctg ttcctggacc gacccgacgc tgatcgcctg
    10501 cgggcgcttc tatcccccgg gggtcaccag cgcggaggcg cgcctgcgcc actacgccgg
    10561 gcagttcgac atcgtcgagg tcgacgcgac ctactacgcg cctccgtcgc tgcgcaacag
    10621 cgagctctgg gtcgcacgca ccgcggacga cttcgtcttc aacgtcaagg ccttcgcgtg
    10681 gctcacccac cacgccgcgg cccccgaccg gctgcccgac tggctgcgca accgcttgcc
    10741 ggacgaggtg cgcggcaagc gctcggtgta cgccagggac gtcggcgagc gcgcgatgga
    10801 gctcgtctgg cagctgcacc gcgaggccct gacgccgctc gccgaggccg gcaagctcgg
    10861 tgcggtgctg ttccagttcc cgccctggtt cgtccgctcg cgcgagaacc gcgactacct
    10921 gaggtccttg cccgagcgcc tgcccggctg gccgctggcg gtggagttcc gcggcggcgg
    10981 ctggctggag gaggactcgg ccgcgaacac gctcgccctg ctcgaggagg ccgggctcgt
    11041 ctacgtcgcc gtcgacgagc cgcaggggtt ccccaacagc acgccgccgc tgagcgcagc
    11101 cacggcgcct ctcggcatgg tccgtctgca cgggcgcaac gccggcacct gggagagcag
    11161 gaccggcgcc gcgtccgacc ggttcaagta cctctacagt gacgacgagc tcgaggagtg
    11221 ggtgccgaag gtgcgcgagc tggcggccgc agccgacacc gtccacgtcc tcttcaacaa
    11281 caactacgag gactggggca tgcagaacgc gcgccgcatg gcgcacctgc tgggagtcga
    11341 gcgccggccg ccgcagacgc agctcgagct gggctgacgc tcgacccgcg cggcggcgtt
    11401 cagcccgggg cggcgagcag ctcgcgcatc tgcgcgtcga cggtgacgcc ccagcgcgtg
    11461 aactcggcga cgaaggcctc gccggggacg acctgctcgg gcacgaagac gccgggcccg
    11521 gcgatgtccc cgcgggcgag catgcgcgcc acgatcgccg gcgggatgcc ggtgtccaca
    11581 tcgccgccgc cggcgccgtg ctccttgtgc gccggaacgg tggcgcgcac gcgccactcc
    11641 acccggcgcc cgtccttgag gccgcgcacc acgccgagca ggtagtcgac gtcgtcgagc
    11701 accacgtcgg cgggcggcgg gatctcgctc accaccttga ggagcacgtc gcgcgggcgc
    11761 acgcgcacgc ccttgacctc gacctcgtcg gtgcgcgtca tgccgatggc gttcaggaac
    11821 cgcatctggc cggtgaactc ggcgggcagc gagagcttga acgtcgccga cctgagcccc
    11881 tggtccttcc aggcgtgcca gaaggtgaac ggctccgagt ggatggtgta gtgcgcctcg
    11941 agcgtgccgg ccggcacgcc gaagtcgagg tcctccaccc cctccccgcc ggcgatgtcc
    12001 atgacccagt ggccgtcgat gaactcgggc gcgaccacgc tgaactcgtc gcacagggtc
    12061 tcgagggagt agggcacgac ccagccgtcg tagccgctcc agtcggtgtc gtcgacgttg
    12121 ccgcagagcg cgtgcgcctc ctcgacgacg tcgagccgct cggccccgta ccgggcgagg
    12181 atgttggtga cgccgggcgc cccgcccatg gcgatgacgg cggtgacgcc cgcctgacgg
    12241 aactcctcgt gcagttcgac ctgctgcttg gtgacgtgga agaggccgcc catgtccgtg
    12301 tagtgcacgc gcgcggccgc ggcggcgtgc atcacggtga tgttccagaa gtgctgcgtg
    12361 gcattgacga gcgcgtcgca gccggcgatc gccgcctgca cgctctcctg atcgcgcgcg
    12421 tcgacgaagc ggcccgtggc cttgccgccg ccgtggctcg cggccacctc tcgcgcctgg
    12481 tcctcgttga agtccccgac gaccacggcg tcgacctcgg gggccagccc gagctccttg
    12541 acggcccagc gacccatggc gcccgcaccc acgacggcga tcttcatcgt cccctcctgc
    12601 acgctcggat gtccgctgcc cgcatccgag cctacgagac tcgccgggcg aagtacagac
    12661 ggctatcatt gcccctgcag cacgtcgatc caccccccgg gaggaagatg accgtggcac
    12721 ggtacggcct gagcgagtcc gagtggcagg cgctcgagga cgaggtcctc gagctgttca
    12781 ccgcgctgct gcgggtcgac acgacgaacg gcaacgagac cgaggcggcc cttgtcgtgc
    12841 aggcgtacct cgccgagaac ggcatcccgg gcgagctcga cggcgagctc cccgaccgga
    12901 agagcctcgt cgccaggctg gacggcgccc gccccggcgc caccctgacg atgatgggcc
    12961 acctcgacgt cgtgccggcg gacgccggcg agtggagcgt gccgccgttc gccggcgtcg
    13021 tgaaggacgg ctacgtctgg ggcctcggcg cggtggacat gaagaaccag gtggcggccg
    13081 aggccgtcgc cctcgcccgg ctcaagcgcg cgggcgcctc cttctccggc accgtgaagt
    13141 acgccgccac cgccgacgag gaggtcggcg agcactgcgg cgtgcggtgg ctctgcgagc
    13201 acaggccgga ggcgctgcgc gccgactacc tcgtcaacga gggcatgggc ggcctgtggc
    13261 tgccggtgga tggccacaag gtgttcctgc tcgccgtggg cgagaaggcg ttcgcgcagt
    13321 tccgcatccg cacccgtggc cgcggcgggc acgggtccgt gcccgagaag gagcgcagcg
    13381 ccgtgctcga cctggcgcgc gccgtgatcg cgctcggcgt cgccgacccg ccggcgatcg
    13441 tctcgggcac gacggcgcgg ttcatcgacg cgctcgtgcc cgaccgcgag ctcgccgcgc
    13501 ggctcaagga tccggccacg gcgcgcgtcg ccggagccga gctccgccgg ctcgacccgg
    13561 tcgtggcggg tctggtcgag ccgctgttcg gcgccacgct caccccgacc gtcctcgccg
    13621 cgggcaaagc cgtcaacgtc atcccgaccc gggcggaggc ctgcgtcgac tgccgcatcc
    13681 tccccgagat ggcgcaggcc gacgtccggg agtacgtcac cacggtcctc gaccccctcg
    13741 gcatcgagtg ggagttcgag tgggtcgacg tgacgacccc caatgcgtcg cccgcctcca
    13801 cccctctgag cgagagcatc gagcgggtgc tgcgccggga cgtcccggac gccgtcctcg
    13861 cacccatgac ctcgagcagt ttcaccgatt cgcgctgggt acgcgaggcg ttcccggatt
    13921 gcgtggccta cggcttcgcg cccttcctcg ccgagagctt ccacgccatg gacggcggcc
    13981 gcgaccacga gccggacgag cgcatcctgg tcgaggacgt ggtgtaccag gcgctcttct
    14041 tcgagcggct cgccggggac ctgctcgcat gagccccgcc ggggaagccg ccgtcatgcg
    14101 cgaccgcttc cgcggtgcgc tgctcggcct cgccgccggc gatgccctgg gcgcaccggc
    14161 ggagttcctc accgcggatc aggtggtcga gaagtacggg gtcatcaccg ggatgatcgg
    14221 cggcggcgtg tacgacgtcg cgcccggcga ggtcaccgac gccacggaga tgatgctctg
    14281 cctcgcggag agcctcgccg agcacggcgc cttcgagccc gaggacatca tggcgcgcct
    14341 cggggcatgg ctggagaccc agccgaggca cgtgagcctc accgtacgcg ccgcgctcat
    14401 aagctaccgc tcgggcacgc actgggacgt cgcctcgcgg cgggcctacg agatcctcgg
    14461 cgggccgacg gccggcaacg ggagcctcat ccgctgcgcc cccgtcggcc tcctctacag
    14521 cggcgacgcg gcggagcgtc acggcgtgtc gctgcgcgaa tccacgctca cgcacttcga
    14581 ccggctcgcc ggctggtcgt gcgccgcctt caacgacctc atctcggcgg cgctcaacgg
    14641 cgagctcgcc gcggacatcc cggccatcgc cagcaccttc gacgaggacg acaagcgcgt
    14701 cgccgccgtc ctgcgcgaca cccccgtggc cgaggcggag gagatcgtca gctcctcgtt
    14761 cgtgctcgac accctgcaga cggcgctctg gaccgtgctg cacggggccg acttcgagca
    14821 cgccgtgacc gtcgccgtga acatgggcaa cgacgccacg gcggccggcg ccgtgaccgg
    14881 ggccctcgcc ggggcggtgt acggcgagtc cggcatcccc gcgcgctggc tcgacgagct
    14941 cacggtgcgc gaccgcgtga ccgccgccgc cgacaagctc gctgcgctcg ccggcgtcgg
    15001 ctgacgcaga cccgctcccc tctcctcccg gacgcggcgg cgaccccggt tgcgaagctt
    15061 gcgttcactc tgtactttac atcgctgacg ttgtcacctt ctgtggggag acatgcaccg
    15121 tggacgagcg tgaacgacgg cgcgccgagc gcctgctggt ggaggagtac ggcctcctct
    15181 tcgaagagat gggcggcacg cgcatggagg gacgcgtcct ggcgtggctc ctcctcgccg
    15241 accccccaga acagtccctg accgggatcg cgcagggact cggcgtcagc aaggccgcgg
    15301 tgagcacagc cgcgcgcagc ctgctgcagt cccgcgccgt ggagcgcgtg agcgagccgg
    15361 ggcaccgcgg cgacttctac cgttccatcc cgggcaagct ggagagcgtg ctccgcctcg
    15421 accacgtcac cgccctcggg cgactcgtcg accgcagcct ggagctcgtg gcggacggcg
    15481 accagacgca gtccaactac gccctccttc gcgagatgcg cgcgttcatc gacttcctcg
    15541 aggccgagat ccccggcctc gtcagccgct ggcagcgtca ccgtgcggcg aaccacaccc
    15601 tcgccgacgg cggcgaccgc gacaacgatc acactggagg cgccacgtga gctggctgac
    15661 gaagatctcc ctgcgcaacc gctccatcgt cggcctggcg gtgctggccg tgctgctcgt
    15721 gggcgcgttc gcgatcaccg gcctcaagca ggagctgatc cccgacctca ccttccccta
    15781 cctcaccgtc gtcaccgtcg accagggctc gtcgcccaag gacgtggagc ggaacatcac
    15841 gacgccgctc gagcaggcga tcaagacctc gagcggcgtc aaggagttcg actcgttctc
    15901 caacgacggg atgagcatca tcaccatcga gtacgagttc ggcacggaca tggacgccag
    15961 gcaggcggag atccagcagg ccgtgtccgg cgtgcagcag atgctgcccg ccaccgccat
    16021 gcctccggaa gtcgccacgc tcaacttcgg cagcatgccg gtcgtgcagc tggcggtgac
    16081 gtcctccctg ccccccgagc agctggcgac gctgctcggc acgcgcgtgg tgccccgcct
    16141 gcagtcgatc gagggcgtgc aggcggtcac cctctcaggc gtgcagcaga tgcagctgaa
    16201 catccagctg aagccggagc aggtcgcggc cctgggcgtg acgcccacgc agatcatgac
    16261 ggcggtcacc caggccaacg tcacgacggg cgccggcagc gtcacctcgg gcagcatcgt
    16321 ctaccccgtc accgtgagcg cgaaggccga gacggagaag gcgctcgagc agctggtgct
    16381 cgcgccctcg agcgccgcgt cggccgcggg cgcggctgcc gcgaccggag cttcagccgt
    16441 ccccgccgag gccgacgcga tggcagccgc ggccgccggc gaggccgcag ccgacaagta
    16501 ccggcagacc gtcaggctcg gcgacgtcgc cgacgtcgag atcgcgccgg cccccctcac
    16561 cgccgtcacc cgcaccaacg gcgagacctc cgtcggcatc tccgtgagca agtccagcag
    16621 cggcaacacc gtcgacatcg ccaacgccgt cgccgacgag ctgccggcgc tcacccgcga
    16681 gctcggcggc gacaccgaga tcaccaccgt ggtggaccag tccgtgttca tcaaggacag
    16741 catccgctcc ctggtgcgcg agggcctcct cggggccgtg ttcgcggtcg tcgtcatctg
    16801 ggtgttcctg cgcagctggc ggtccaccgt catcgccggt ctctcgatcc cgctcagcgt
    16861 gatcggcgcg ctcatcatcc tgtggtcgcg cggcgagtcc ctcaacatgc tcacgctggg
    16921 gggcctcacc atcgccatcg gccgcgtgat cgacgactcc atcgtcgtca tcgagaacat
    16981 ctaccgccac ttgcaggagg gcgacagcgt ccggcgcgcc gcctacaccg gcacgcgcga
    17041 agtggccggg gccatcacgg cgagcaccct gaccaccgtc gccgtcttcc tcccgctcgg
    17101 catggtggcc ggtctcgcct cggagttctt ccgcccgttc gcgctcacgg tgacgttcgc
    17161 gctgctgtgc agcctcctcg tcgcgctggt cgtcgtcccg gtcgcctcga cctggctgct
    17221 ctccaagcgc caggtggggc accgcgacga gagggagctc accgcgctgc agaacgccta
    17281 cctgccggcg ctccgcttcg ccctgggcca caaggcgatc acgctcatcg ccgcggcggc
    17341 gatcttcgtg ggcaccatgc tgttcacgcc gctgctcaag accaacctgt tcgacagctc
    17401 ggagcagccc accatgagca tcacccagac catggagccg ggcacgagcc tggacgccac
    17461 gatggccgcc gcgtccaagg tcgaggaggt cctcaagagc acggagggcg tcgagatcta
    17521 ccaggtcacc ggcggcagca ccggcagcct gttcggcgcc gggggtggca ccaacgcctc
    17581 gtccagccag gccacgttcg ccgtcacgac cgaccccgac gccgacaaga acgccatcat
    17641 ggacgaggtg cgcgccggcg tggccgagct cgagaacgcc ggcaccatca gcgtgaccgg
    17701 tgacgactcc tcgtcgtcca tgggcggcat gtcgctcatg gaggtccgca tctccgccgg
    17761 cgacccccag gtgctcaagg aggccaacga catcgtcctc gcagaggtcg agaccgtcga
    17821 cggactcgcc gacgtcacct ccaacctcag cgaaggccgc ccgtccgcca cgatcgtcgt
    17881 cgaccaggcg aaggcggcgg cggccggcgt caccccggcc accctgagcc agtacacgac
    17941 gctggtgctc aacggctacc cgctcggcac cgtgcccacc acggccggga cactgccggc
    18001 gcagctgatg gtgggagaga tcgtcgtgcc gccgctcccc ggcgccgtga gtcaggtgct
    18061 cgcccggctg ccggtcgccg gcacgtccgg catggtccct ctcggcgaca tcgccgaggt
    18121 cgtagaggtc aaggcgcccg tgcaggtgac ccacgtcgac ggcgcgcgca cggcgagcat
    18181 caccgcgacc gccgtgaaca acaacatcgg cgcggcctcc acggccgtcg aggacgcgct
    18241 caaggacgtc cagctgccgg agggcgccac gtgggagctc gccggcgcca ccgaggccac
    18301 caacgacgtc ttccgcacgc tgggcatcgc catgctcgtc gcgatcctgc tcgtgtacat
    18361 catcatggtg gcgaccttcc gcagcctgct gaacccgctg atcctgctgg tcagcatccc
    18421 gttcgccgca gtgggcgcgg tactggccct cgtcatcacc ggcaccagcc tcggcatgcc
    18481 gagcctcgtc ggcctgctca tgctgatcgg catcgtcgtg accaacgcca tcgtgttgct
    18541 cgacctcgtg gagcagttcc ggcgcaaggg aatggacgcc cgcaccgccg tcatcgaggg
    18601 cggccggcga cgcctgcggc cgatcctcat gaccgccatc gccacgatcc tggcgctcac
    18661 gccgatggcg ctcggcatcg gcggcgaggg cggctttctc tcgaccccgc tcgccgtggt
    18721 cgtgatcggc ggcctcttca cctcgacgtt cctcacgctg atcctggtgc cggtgttgta
    18781 cctggcgttc gaccggctgc ggcccgccgg cgcctacgcg ctggacgacg aggactacac
    18841 gacgatggcg gcggagccgg ccccggccga ctgacgccgc cgaggccggc cgcagcgcgc
    18901 cggcggagca cgcggcgacg tccgccggac aacaccgtcc ggcggacgtc gccgttctgc
    18961 tgcagtactc ttctctcagc ggccggcccg agacccggcc cgacgacggc acgacgagag
    19021 gagagccagt ggcgaagcca gccccaaaga agagagccgc caaagcgagc gtcgcgacga
    19081 aaccgcgcac ggcggccgag atgcagcgcc ccgtcccgcg cgagctcacc cgcgccgagc
    19141 tcatcgacga gctcaaccgc gacctcgcca aggagttcca ggcgctggtg cagtacgtgc
    19201 agcacgcggc ggccatgacg ggcccgcagt acgacgccat caaggccgag ctggtcgtcc
    19261 actccaacga ggagcacgcc cacgcgatct cgctggccga ccagatcgac ttcctcggcg
    19321 gcgtgccggg cgtggacgtg gccgaggcgc tcatctcgcc cgacgccaag accatgctcg
    19381 agcaggacct cgacggcgag ctcgacgcga tcgcccgcta ccgcgagcgc atcgcccagg
    19441 cggagatgct gcaggagtac ggcctgcggc gcgcgctcga ggacatcctc atcgtcgagg
    19501 aggagcacgc ccgcgacctg caggacgcgc tcgaccgctg acggctgcgg cggaggcccg
    19561 ggcgcgacgc tacgccgtcg cccgggcctc caccgccttc gcccggcgtc gccggcccca
    19621 gcccggcgcc agcgccagga accagacggc gccggccagc cccaccacga gcagggcgac
    19681 gaacgcctcg cgcccgccca cggtgtccat cgcccagccg ccgaacgcag gcccgagggc
    19741 ggcgcccccg ttccacacca cggtccacac gcccatgtag cgcccgcgca cggcctcggg
    19801 cgcgagctcg gccacctccg ccgacgccac cggcgacagg agcgtctcgc ccaggctcag
    19861 gatggcgatg aaagccacga gggaccacac cgggccggcg aacgcggacc cgccgatgcc
    19921 gacggcgagc agtgcgctgg aggcggcgag cagcagcatg cgattgcgcc ggcggagcgc
    19981 cgtcaccagc gggatctgga cgacgaccac gatgagggcg ttgagcgtga gcaggtacgc
    20041 ccacgttctg ctgggcacgc cgaggaagtc ggtgatgtac acggcgtaga tggagccgaa
    20101 gttgccgatg cagaacacgg gcaggacggc ggcgaggcag aagacgatga agacccggtc
    20161 gtgcagcacc gcgcggtacc cggcccgccc gcgctcgtcg acccgcctga cgcgcaccgt
    20221 ggcgctcgcc ggccggctct cccggatcca caccgccatc atcaccacca tcgccaggca
    20281 cccggctgcg gcggagagga agagcacccg gaagctgacg ccgaacccga gcgcctgccc
    20341 ggccatcacg ggtccgacga cgacgccgaa gttcatcgcc gcccgcgtca cgccgaaccc
    20401 ctcctgccgc cgctcctgcg gcagcaggtc ggcgatcatg gcgttgctcg cggtctggaa
    20461 gagcggccag ccgaacgcgc tctccagcgc gacgagcgcg gccacctgcc agagctcggt
    20521 gacgaaggcg aagcccacga accagacgac gccgacgagc accgagacca tgatgatcac
    20581 gcgccgcccg agcttgtccg tgagctgccc gccccagaac tggaccggca tcacggccag
    20641 cggcaccagg ccgaagacga cgccgatcat ggtcatcgac gtccccaggt actggcgcag
    20701 gtagatggcc tcgaacggga aggcgagggc ggcggcgccg acgtagatga agatgcccag
    20761 cgtcagcacc cagaactggc cggggaagtc caggtaccgt ctcagtcggg cgagcatggc
    20821 cccatgctat ccgagggcac gacgggcggc atgtacgcgc cgaaaccatc gctgctgccg
    20881 tcacgaaccg tcagcgcaag gccccgtgag gctcgcgaac ctgcgcgccg cactcgccgg
    20941 aacctccatg atttgacgct ccctccgcca gcggctaggt tccggccatg tacctgagcg
    21001 tgcaacaggc ggcgcggcgt ctcggcgtct cgccggtgac gatccgccgc tggacggcga
    21061 ccggcttcct cccctgcacg cgcaccgccg gcgggcaccg gcgcatcgac gaccacgaca
    21121 tcgacgacct cgccaaggcc atcgggggca gcaaccacct cgccgcccag ctcgcccgcg
    21181 agcgcgaggt cgacgtgctc atcaacacgt cgaccgcgct ggccgtgaag ctcgacctca
    21241 ccgagctgct gctcgagatc gccatgcgca tgacgagcct gttccagtgc cacctgtgcg
    21301 cgatctcgga gtacgacccc gaggacgacg cggtgcgcac gcttgccgag tacgacaacg
    21361 ccgggcggca actcctcgac gcgagcccgt accggttgcg cgagttcccg ctcacgcgcc
    21421 gggtgctcgc cgagcaggtc accgccgtgg tcaacgtcga cgacccgcac gccgaccccg
    21481 ctgaagtggc ggagttgcgg cgggagggag accgcagcct gctcatggtg ccgctcgtcg
    21541 tgcagggccg cagcctcggg ctgctcgagg tggtggacca gaaggtggcg cgccagtact
    21601 cccggcagga gctccgcctc gcccaggcga tcgccggtca ggcggcggtg gccatccaga
    21661 acgccaaact gttcgccgag cggcggcgca gcgacgagga cgtcgcgtac ctgcgcgaag
    21721 ccctgagcac cctcagcgcg cacgtcccgc gcctgggcga gggcagccgc cccggcgacg
    21781 tgctcacgac cgtggcggac gccgtctgcg aggcgctcgg cgccatctcg tgcgtggcca
    21841 gcatgggcgg ggagagcgcc ggcgccttcg gcgccggtcg ggtcgccgcc ccctccggtt
    21901 cagacgccgc gcgcggcgcc aacgccagcc tgctcgtcgt ccgcgacccc tccggccgca
    21961 ccgacctcac gctcgcggtg acgctgccgc acccccccgg cgagggccag gaggagctgc
    22021 tcgacttcgt ggcggcggtc gccgtcctcg tcgcgggctg
//
LOCUS       DASUMX010000069        14695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_594485, whole genome shotgun sequence.
ACCESSION   DASUMX010000069 DASUMX010000000
VERSION     DASUMX010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 14695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14695
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594485"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..1454)
                     /locus_tag="VFZ86_03205"
     CDS             complement(12..1454)
                     /locus_tag="VFZ86_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641329.1"
                     /translation="MFGRIFGRGKDDQDQAVCAECGRTLLAGEWTQTVVDEHGEERLI
                     CSLCGQAYTGGSEPVAAAPTPANNGRVRETRSEARYDERPAQPEAPARAVRAESDAFW
                     QALKEKDAQIEELKAELARAEAERQELAGQFARLGAAGAAGAAAPDEPGSLTGDSSEP
                     GERTWGETPAEFAAEMAALNEADAASDRPQDVWDEPAQDLAVEAPPAEELVEGASATG
                     EPAEAAVEEDAVSAPDAESPAGAPAAVSATVFEDTQPIPAVPEDLDEDEGSTGEIAAA
                     EAEAAFAAGAALPGEPTEQEPAAPSAAEAEAVAASLTLLQRGVDLLNVSGVPRKIAET
                     NEQLGLPHVHAGFDDDIVAVSFMWTMGWYRFHVDIDSGDVTMHDRGYEELTLQPNAGV
                     RADGTVQLAPAQISRAAAQRAQAAPAPASPEPAQPAAQPLAAPAEPETPREAAQKPPE
                     FLSKSLLGQRSDDETASWEQTTARDFDWDR"
     gene            complement(1544..1780)
                     /locus_tag="VFZ86_03210"
     CDS             complement(1544..1780)
                     /locus_tag="VFZ86_03210"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HEX5641330.1"
                     /translation="MELVTVTTVSDAGLANVVVGVLQQAGVEAVVGGSGAPDVYPTPS
                     VHPYHILVNEDDLAKARDVLDQFETMPDDADDDE"
     gene            complement(1886..2731)
                     /locus_tag="VFZ86_03215"
     CDS             complement(1886..2731)
                     /locus_tag="VFZ86_03215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HEX5641331.1"
                     /translation="MYFGAHVKSSGGVWHAIENGVDIGAEAVQFFAGSPRTWKPTLYK
                     DADSAKFRELRAASPIRFAVIHTIYLINLASENEDFYEKSVRSLCGAVTAAEQLGADA
                     IVTHIGSHQGSGFAAGLERVQAALRTALAESEGSPVRLLLENTAGAGGTMGVDFRELG
                     QIIDATGGDPRLGICVDTAHIFESGVDLRGREGVDDLLRELDEGCGRERLVMFHLNDS
                     KTALGSNRDRHENIGDGDLGDDAFRLLVNEPAFADLPGILEVPGLDGQGPDRENLARL
                     RELVA"
     gene            complement(2747..4825)
                     /locus_tag="VFZ86_03220"
     CDS             complement(2747..4825)
                     /locus_tag="VFZ86_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015259750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEX5641332.1"
                     /translation="MTERDAPLRDVRQLDYVWIPMRDGARLAARVWLPADAESDPVPA
                     ILEALPYRLSDGTAARDARVHPYWAARGYACVRVDLRGSGESDGVLEDEYHPQEQDDL
                     LEVIAWIAAQPWCSGGVGMTGISWGGFNSLQLAARRPPALRAVITLMSTDDRYADDVH
                     YKGGCVLATDLLHWSTCMLHWQCQPPHEAAVGARWRELWEQRLASARPWIRTWLAHQR
                     RDAYWRHGSVCEDYAAIEVPVYAIGGWTDGYTNSVLRLLEGLGGPRKGLIGPWPHALP
                     HAVTPGPAIGFLQEALRWWDFWLKGIETGVMNEPVLRVWMQESVPPVPWMDEVPGRWV
                     AEDAWPSPRLEPRTLRLDADGVLRDAADGAAPDEQAAAAHGAGGGRLAIRGAQLCGAD
                     AGAWCAESQPSDFAPDQRAAEGQSLCFTTAPLPQDLEILGHPRLTLRFASDRPLALVA
                     ARLDDVAPGGVSRLVTTQVFNLTHLTSHEHPGRLEAGREYEATIALDAIAQAFPAGHR
                     LRLALSPTYWPWAWPSPEPVTLTVVAGESSLELPVRPQRPEDARLRPFEPPFVPEGLG
                     ETTVGGGRGGRSYTRDLAADDITWTFRYVDGGEVVLPNGWTSEEWNTVSYGLREGDPL
                     SARVSVEVESVLLRGAQGRFHIVARGEMTCDADTFYVADEVAVHEGEEGDEREVFTRS
                     WWHQAPRDFV"
     gene            4990..5229
                     /locus_tag="VFZ86_03225"
     CDS             4990..5229
                     /locus_tag="VFZ86_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641333.1"
                     /translation="MDQQSMQRCRTCPAFAKIPGPGDSGTCRAAPPVVLQAENYFRGS
                     TRELPFGCFPVVGAFPLVTEEDYCMSHPGNRSRLM"
     gene            5366..8161
                     /gene="asnB"
                     /locus_tag="VFZ86_03230"
     CDS             5366..8161
                     /gene="asnB"
                     /locus_tag="VFZ86_03230"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEX5641334.1"
                     /translation="MCGIFGFAGSPDRQLLGRMGAAIVHRGPDDAGAFETPSVSLGHR
                     RLSIIDREGGHQPVANEDETVWLVYNGEVYNYRELRAELEAAGHVFRSHCDSEVIVHA
                     YEEWGPGCAARFNGMWAFAVADLREAGEDGRGGKLVLNRDHFGIKPLYYARSPQSGRL
                     LFASEIKALLQDPELVAEPDEQMVFEYLQHGFHDHRAETFFRGVYHVPAATWIELPLG
                     GAAGAPAGAGAGAEAAPAPGRLAAPLDSTAYWTPVLSRDGGDDPADFRRRFRESVERR
                     LVSEVPVGSCLSGGLDSTTIISFMSELLKEDVPDAASLRGQLKTFSAVFDGDPIDERE
                     YIETAVESTGADTTYTNPTSHEFIGELRDFVWHQEEPIVSTGPYAQWCVMRSAREQVT
                     VLLDGQGGDELIAGYVPYQLVYLRQLRKQGDYDLLRREATASRDVLMPLAKRRLKQRR
                     KQLPTRKLLRQEFLARTTDPGYGRSQSDLKERLLQDLLTYSLPCLLRYEDRNSMAFSI
                     ESRVPYLDQEFVDHILSLPEDAIVRHGWSRWILRAALKGTLPEKIRLRRWKVGFTTPE
                     MRWIKARRAAFTSLYQSPAFQARPYWDGEAVVQAFRACCKGQVEESMFFWRAANVELW
                     LREFVDRSVVIEDADVEAALGAPVPVGPVHRGSIAEAGDALVPALLGAAGASPATAEA
                     ERLLAGHAPNEEKHLFAVAGGTVFARLPLRTSLVGRGDDLDELMRREIAAHIKPGDLV
                     VMAEKPIAASQGRSYALDEIHPTKLARVLSKAVTKTPHGIGLGIPETMQLAIDEAGAP
                     RIVAAAAVSAAGKAVGKRGLFYKVAGADVEAIDGPTWNTLPPHNTHAKLGPADPDGVA
                     AHLADVLSDAVGGRVEFVVIDANDLTAAVLGASQGADRALARRLMRDNPLGQGHEQTP
                     VCALRALGPLTGAVQQG"
     gene            complement(8651..10543)
                     /locus_tag="VFZ86_03235"
     CDS             complement(8651..10543)
                     /locus_tag="VFZ86_03235"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HEX5641335.1"
                     /translation="MRRVRDHLLVIIVGLAVPLLAGAAHAAPATAVTPAPHGPERAGA
                     RFVPRQALVRFHQGVSVGVMDRIALAHGARVTKSLPRAPGGGRSAVVRSASLSTAELV
                     AELAADPRVDRAEPDYLLRLEATPDDPDLPALWGMPRIRAPRAWDLTTGSSSVVLASV
                     DSGVDYTHPDLAANMWRNPGEIAGNGLDDDANGYVDDVHGIAEAPDAADPSDPMDVQD
                     HGTHTAGTMAAVGDNGMGVTGVAWKASIMALRFLGADGSGATSDAISCITYAVAMKER
                     GVNVVAINASWGNGEASAFLRWAVETAGDAGIVVVAAAGNGGGDKIGDDNDAVPYYPA
                     SYDSPNIIAVAASDGADQLAEFSDHGAASVDLVAPGVGIRSTVPASTDPGGYVSRNGT
                     SMATPHVTGTIALCAALYPEESVAERIARVLASADPVASLTGKCATGGRLDVLGAVDT
                     GAPVTTTTGVDGAWHASAVTVSFSALDAGSGVAYVESSLDGGDWVRGATREVTGNAAH
                     ALAFRAVDDAGNTEATRRVTVRVDAGRPTPLALGDATARQGRRATLRYRIDDVTPRAT
                     CRIRIYRGTRTVKTLKPGVVRTNQNATHAWTCRLAPGRYTWKVFATDQAGNTQSKPGA
                     RRLTVR"
     gene            10708..11628
                     /locus_tag="VFZ86_03240"
     CDS             10708..11628
                     /locus_tag="VFZ86_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5641336.1"
                     /translation="MRKQYDPPKGVLAVDGVSFDIAEGEIFSLLGPNGAGKSTTISML
                     SCLLRPTGGDATVAGHSVVSNAQAVKETIGVVPQDIALYPDMSARENLVFWGRMYGLS
                     GDTLKQRVDEVLELITLVDRQKDRIEKFSGGMKRRVNIGVALLHKPRVLYLDEPTVGI
                     DPQSRRSILDGILALRDQGMTILYTTHYMAEAQELSDRIGIVDQGKMIALGTHEELVK
                     IVGQAARVTARVDHSLEAMAERWREVAGVAQVTIEEDEAVLLTDDANEVLPRLFEAAA
                     AAGSRITEIDVREPNLEMVFLHLTGRALRE"
     gene            11684..12925
                     /locus_tag="VFZ86_03245"
     CDS             11684..12925
                     /locus_tag="VFZ86_03245"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5641337.1"
                     /translation="MMKILAIAWKDLRSTARNVPALVMMLAAPLALAALLGFAFGGGG
                     GFDIAATKVVVANLDGGGAGAGQTAGAAIQTVLTGEGLEDVLETSTRSSAAAARQAVD
                     DGEAAVAVIVPEDFSAVVYGGDPAARSQVEIYENPTREIGGSIVEGVVGQVLADINGA
                     RAAAAGAVALAAAGSDPIDPRVLAARAAEQFSHDGGVSAGLQIGQRAPRVGATEEEVS
                     VTGLILAGMMVFFMFFGAANVARTILTEDRDGTLPRLFTTPTRHGTIIGGKFVSTFLT
                     VLAQAIVLLVAGRLIFGIQWGPLDAVVLLTVVTAGVAGGLALLVIAFTRNAAQAGAIG
                     AGVYLVLALLGGNFTGTAQTGTTYAFIQRFTPNGWLLHGWDTAMRGGGAADLRWQLLV
                     PLGFAVAFFFFAVLRMRRRFA"
     gene            12936..14144
                     /locus_tag="VFZ86_03250"
     CDS             12936..14144
                     /locus_tag="VFZ86_03250"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5641338.1"
                     /translation="MKLAAVLARKDLLQTVRDKLSFIFILVMPLAFTLFFGLLFGAGS
                     SVDQLPLAVWDADGGAAATQLVAALEESPVVRVVVKEGNEFETWMADERAAAGLLVPE
                     GYSAAMAAGERADLTLVSIQGSSGAATVASEVRTLAGEQVTVELASRVAAEVVWSTRS
                     MPAGAQEEVVADAAAQARPIVAQALASPAVSTDVVEAGVAAGQIPSGFVLSSPGMLVN
                     FILFSLLTAGIALIVERQNGTLQRLMTTRLRRWQLIGGKAAGMFLLTFVQQVLLIAVA
                     QIFFGVDYLRDPAALLLMMVSLSLVASTLGLLLASVLKSEQALIATTVLVSMGISALS
                     GAWFPLEVTGAAFQSVGHLLPTAWILDGLRGIVVRGFDVADVLPALGVSVAWAAAFFA
                     LAVWRFRLSD"
     gene            complement(14149..14463)
                     /locus_tag="VFZ86_03255"
     CDS             complement(14149..14463)
                     /locus_tag="VFZ86_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005038253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641339.1"
                     /translation="MASAEIESGICGFCTTVKTTSEGRTVRVEFETECGYVEKLAEAL
                     TEVDPYREISYRGEGPVTLRLAAEHLVHPACPVPSGIIKAIEVEAGLALPKDASIKPS
                     KD"
BASE COUNT         1950 a         5340 c         5289 g         2116 t
ORIGIN      
        1 gtgcccgcgg ctcagcggtc ccagtcgaag tccctcgccg tggtctgctc ccacgacgcc
       61 gtctcgtcgt cgctgcgctg gccgagcagc gacttgctga ggaactccgg cggcttctgc
      121 gcggcctcgc gtggcgtctc cggctcggct ggggcggcga gcggctgggc cgcgggctgc
      181 gccggctccg gcgacgccgg ggcgggcgcg gcctgagcgc gctgggcggc ggcgcggctg
      241 atctgcgccg gcgcgagctg caccgtgccg tcggcgcgca cgccggcatt gggctgcagc
      301 gtgagctcct cgtacccgcg gtcgtgcatg gtcacgtcgc cgctgtcgat gtcgacgtgg
      361 aaccggtacc agcccatcgt ccacatgaag ctgacggcga cgatgtcgtc gtcgaacccg
      421 gcgtgcacgt gcgggaggcc gagctgttcg ttggtctcgg cgatcttgcg cggcacgccg
      481 ctcacgttga gcaggtccac gccgcgctgc aggagcgtga gcgacgcggc cacagcctcg
      541 gcctccgcag ccgacggggc cgcgggctcc tgctccgtgg gctctcccgg cagggccgcg
      601 ccggcggcga acgccgcctc ggcctcggcc gcggcgatct caccggtgct tccttcgtcc
      661 tcgtcgaggt cctcggggac ggccgggatc ggctgcgtgt cctcgaacac cgtcgccgag
      721 accgcggccg gtgcgccggc gggcgactcc gcgtcgggcg ccgagacggc atcctcttcg
      781 acggccgctt cggcaggctc gcctgtggcg gaggcgccct cgacgagctc ttcagcgggc
      841 ggcgcctcga ccgcgaggtc ctgcgccggc tcgtcccaca cgtcctgggg ccgatcggac
      901 gctgcgtccg cctcgttcag cgcggccatc tcggcggcga actcggcagg cgtctcgccc
      961 cacgttcgct caccgggctc cgaggagtcg ccggtgagtg agccgggctc gtccggggct
     1021 gccgccccgg ccgctccggc ggcgccgagg cgcgcgaact ggccggccag ctcctggcgc
     1081 tcggcctccg cgcgcgccag ctcggccttg agctcctcga tctgcgcgtc cttctccttg
     1141 agcgcctgcc agaaggcgtc gctctcggcg cgcacggcgc gcgccggggc ctccggctgc
     1201 gccggccgct cgtcgtagcg cgcctcgctg cgcgtctcgc gcacgcgtcc gttgttggcg
     1261 ggggtgggcg cggccgcgac gggctcgctg ccgcccgtgt aggcctgtcc gcacaaggag
     1321 cagatgagcc gctcctcgcc gtgttcatcg acgacggtct gggtccattc gccggcgagg
     1381 agggtgcggc cgcactcggc gcagacggcc tggtcctggt cgtccttgcc gcggccgaag
     1441 atgcgtccga acatcgtcac tcccgtgtct ggaacgcccc gcagggccga aaaaacgctg
     1501 caagaaggca catgatagca gacggcgggg agcccggccc gggtcactcg tcgtcgtcgg
     1561 cgtcgtccgg catcgtctcg aactggtcga gcacgtcacg cgccttggcg aggtcgtcct
     1621 cgttcacgag gatgtggtac gggtggacgg acggcgtcgg atacacgtcg ggcgcgccgg
     1681 acccgccgac cacggcctcc acgccggctt gctggagcac cccgacgacg acgttggcaa
     1741 ggccggcatc gctcacggtg gtcacggtca cgagttccat ggcgtcacct cgctgtagtc
     1801 ggtgtgcacc ccgggcagcc ggagtatacc cgcggccggg tcgtggctct cagcggcgcc
     1861 gtcgcgtcga cggcgcccgc cgccgtcagg ccacgagctc gcgcaggcgg gccaggttct
     1921 ccctgtccgg accctggccg tcgaggcccg ggacctcgag gatgccgggc aggtcggcga
     1981 acgccggctc gttgacgagc aggcggaagg cgtcgtctcc caggtcgccg tcaccgatgt
     2041 tctcgtgccg gtcgcggttg gagccgagcg ccgtcttgga gtcgttcagg tggaacatga
     2101 cgaggcgctc gcggccgcag ccctcgtcca gctcgcgcag gaggtcgtcg acgccttcgc
     2161 gcccgcgcag gtccacgccg gactcgaaga tgtgggccgt gtccacgcag atgccgaggc
     2221 gcggatcgcc gccggtcgcg tcgatgatct ggccgagctc gcggaagtcg acccccatgg
     2281 tgccgccggc gccggccgtg ttctcgagca gcaggcggac cggggatccc tcgctctcgg
     2341 cgagcgccgt gcggagcgcc gcctggacgc gctcgagtcc ggccgcgaag ccggagcctt
     2401 gatgtgagcc gatgtgcgtg acgatggcat cggctccgag ctgctccgcg gccgtcactg
     2461 cgccacacag cgacctcacc gacttctcgt agaagtcctc gttctcgctc gcgaggttga
     2521 tgaggtagat cgtgtggatg acggcgaagc ggatgggcga agccgcgcgc agctcgcgga
     2581 acttcgccga gtcggcgtcc ttgtagagcg tcggcttcca ggtgcgcggg ctgccggcga
     2641 agaactgcac cgcctcagcg ccgatgtcca cgccgttctc gatggcgtgc cacacgccgc
     2701 ccgacgactt cacgtgcgcg ccgaagtaca tctcctgccc tccctgtcag acgaagtcgc
     2761 gtggcgcctg gtgccaccac gacctcgtga agacctcgcg ttcgtcgccc tcctcaccct
     2821 cgtggacggc gacctcgtcc gcgacgtaga acgtgtccgc gtcgcaggtc atctccccgc
     2881 gcgcgacgat gtgaaaccgg ccctgcgcgc cgcggagcag caccgactcg acctccaccg
     2941 acaccctggc ggacagcggg tcgccctcgc gcaggccgta gctcacggtg ttccactcct
     3001 cgctcgtcca gccgttgggg aggacgacct cgccgccgtc cacgtaacgg aaggtccagg
     3061 tgatgtcgtc ggcggcgagg tcgcgcgtgt aggaccggcc gccgcggccg ccaccgaccg
     3121 tcgtctcgcc gaggccctcg gggacgaagg gcggctcgaa cggcctgagc cgggcatcct
     3181 ccggccgctg cggccgcacc ggcagctcca gagagctctc gccggcgacc accgtgagcg
     3241 tgacgggctc gggcgagggc cacgcccacg gccagtaggt cggagagagc gccagccgca
     3301 ggcggtggcc ggcggggaag gcctgggcga tcgcgtccag cgcgatcgtc gcctcgtact
     3361 ccctgccggc ctcgagacgc ccggggtgct cgtggctcgt caggtgcgtc aggttgaaga
     3421 cctgtgtggt gacgagcctg gagacgccgc ccggcgccac gtcgtccagc cgggcggcga
     3481 ccagcgccag cggccggtcg ctcgcgaagc ggagcgtgag ccgcgggtgg ccgaggatct
     3541 cgaggtcctg cggcagcggc gccgtggtga agcagagcga ctgcccttcc gccgcccgct
     3601 ggtcgggcgc gaagtcgctc ggctggctct cggcgcacca ggcgccggcg tcggcgccgc
     3661 agagctgggc gccgcggatg gcgaggcggc cgccgccggc gccgtgcgcg gccgccgcct
     3721 gctcgtccgg cgcggcgccg tccgccgcat cgcgcagcac cccgtccgcg tccagtcgca
     3781 gcgtccgggg ctcgaggcgc ggagacggcc acgcgtcctc cgccacccag cgccccggca
     3841 cctcgtccat ccacggcacg ggcggcacgg actcctgcat ccacacgcgc agcacgggct
     3901 cgttcatcac acccgtctcg atgcccttga gccagaagtc ccaccagcgc aacgcctcct
     3961 ggaggaaccc gatcgccggg ccgggcgtga cggcgtgcgg gagggcgtgg ggccacgggc
     4021 cgatgaggcc tttgcgcggc cctccgagac cctcgagcag gcgcagcacg ctgttcgtgt
     4081 acccgtcggt ccagccccct atcgcgtaca ccgggacctc gatggccgcg tagtcctcgc
     4141 acacggagcc gtgcctccag tacgcgtcgc gccgctggtg cgcgagccag gtgcggatcc
     4201 agggccttgc ggacgcgagc cgctgttccc acagctcgcg ccaacgcgcg ccgaccgccg
     4261 cctcgtgcgg cggctggcac tgccagtgca gcatgcaggt gctccagtgc agcaggtccg
     4321 tggccagcac gcagccgcct ttgtagtgca cgtcgtcggc gtagcggtcg tcggtgctca
     4381 tcagggtgat gaccgccctg agcgccggcg ggcgcctggc ggcgagctgg aggctgttga
     4441 agccgcccca cgagatcccg gtcatgccga cgccgcccga acaccacggc tgggcggcga
     4501 tccaggcgat gacctcgagc aggtcgtcct gctcctgggg gtggtactcg tcctccagca
     4561 cgccgtccga ctcgccgctg ccccggaggt cgacgcggac gcaggcgtag ccgcgcgcgg
     4621 cccagtacgg gtggacgcgc gcgtcgcgcg ccgccgtccc gtcgctgagg cgatagggca
     4681 gggcctcgag gatggcgggg accgggtcgg actcggcgtc cgccgggagc cagacgcgcg
     4741 ccgcgagccg cgccccgtcg cgcatcggga tccaaacgta gtccagctgg cggacgtcac
     4801 gcagcggcgc gtcgcgctcg gtcatcgtcg acctccaggt cggctcccgg gaacgacccc
     4861 gagtgtaccg ggcgcccgcg gcgggcggca acggagcggg tccgcgcgcc cgccggcgcg
     4921 gcgcgccggg gaggacgcgc gccgcggccg tggaatatct ggcaggtcgc gtcacccaca
     4981 ggaggtcaca tggaccagca gagcatgcag cgctgccgca cctgtccggc gttcgccaag
     5041 atccccggcc cgggcgactc cggcacctgc cgcgccgctc cgccggtggt cctgcaggcc
     5101 gagaactact tccgcggctc cacgcgggag cttcccttcg ggtgtttccc cgtggtcggc
     5161 gcgttcccgc tggtcacgga agaggactac tgcatgtcgc atccggggaa ccgctccagg
     5221 ctcatgtgac ccgcgggcgc cgcgcgcccg ccccgtcgcc ggccgctcgc tcgggccgtc
     5281 tcgccggtgg taaggttgac cggcgcggag gccggcaggg cgaccgcgcc gtcagtggtc
     5341 ccagacccca ggagagcatc cgcccatgtg cggcatcttc ggcttcgccg gctcgcccga
     5401 ccggcagctt ctcgggcgca tgggcgccgc catcgtgcac cgcggcccgg acgacgcggg
     5461 cgccttcgag acgccgtccg tcagcctggg ccatcgccgc ctcagcatca tcgaccgcga
     5521 aggcggccac cagcccgtgg ccaatgagga cgagaccgtc tggctggtct acaacggcga
     5581 ggtctacaac taccgcgagc tgcgtgccga gctcgaggcc gcaggccacg tgttccgctc
     5641 gcactgcgac tccgaggtca tcgtccacgc ctacgaggag tgggggccgg gctgcgccgc
     5701 gcgcttcaac ggcatgtggg cgttcgccgt cgccgacctg cgcgaagcgg gtgaggatgg
     5761 gcgcggcggc aagctcgtcc tgaaccgcga ccacttcggt atcaagccgc tctactacgc
     5821 gaggtcgccg cagagcggcc gcctgctctt cgccagcgag atcaaggcgc tgctgcagga
     5881 tccggaactg gtcgccgagc cggacgagca gatggtgttc gagtacctgc agcacgggtt
     5941 ccacgaccac cgcgccgaga cgttcttccg cggcgtgtac cacgtcccgg ccgccacctg
     6001 gatcgagctg ccgctgggcg gggccgccgg agcgcccgcc ggcgccggcg ccggcgccga
     6061 agccgctccg gcgccgggca ggctcgccgc gccgctcgac agcacggcgt actggacacc
     6121 ggtgctgtcg cgtgacggcg gcgacgaccc ggccgacttc cgccgccgct tccgcgagag
     6181 cgtcgagcgg cgcctcgtct ccgaggtccc cgtgggttcc tgcctctccg gcggcctgga
     6241 ctcgacgacg atcatcagct tcatgagcga gctgctcaag gaggacgtgc ccgacgcggc
     6301 gtccctgcgc ggccagctca agaccttcag cgccgtgttc gacggcgacc ccatcgacga
     6361 gcgcgagtac atcgagaccg ccgtcgagag caccggcgcc gacaccacct acaccaaccc
     6421 gacgtcgcac gagttcatcg gcgagctgcg cgacttcgtc tggcaccagg aagaaccgat
     6481 cgtcagcacc ggcccgtacg cgcagtggtg tgtgatgcgc tccgcgcgcg agcaggtcac
     6541 cgtgctgctc gacggccagg gcggcgacga gctgatcgcg ggctacgtgc cgtaccagct
     6601 cgtgtacctg cgccagctgc gcaagcaggg cgactacgac ctgctgcgca gggaagcgac
     6661 cgcctcgcgc gacgtgctca tgccgctggc aaagcggcgg ctcaagcagc ggcgcaagca
     6721 gctgccgacg cgcaagctgc tgcggcagga gttcctcgcc aggaccaccg accccgggta
     6781 cgggcgctcc cagtcagacc tcaaggagcg cctcctccag gatctgctca cgtacagcct
     6841 gccgtgcctg ctgcgctacg aggaccgcaa ctccatggcg ttcagcatcg agagccgggt
     6901 gccgtatctc gaccaggagt tcgtggacca catcctgagc ctgccggagg acgccatcgt
     6961 gcgtcacggc tggagccggt ggatcctgcg cgccgcgctc aaaggtacgc tgccggagaa
     7021 gatccgcctg cgccgctgga aggtcgggtt cacgaccccg gagatgcgct ggatcaaggc
     7081 gcgccgggcg gcgttcacga gcctctacca gtcgccggcg ttccaggcgc ggccgtactg
     7141 ggacggcgag gccgtcgtgc aggcgttccg cgcctgctgc aaaggccagg tggaggagag
     7201 catgttcttc tggcgcgccg ccaacgtcga gctctggctg cgcgagttcg tcgaccggag
     7261 cgtggtcatc gaggacgccg acgtcgaggc ggcgctgggc gcgcccgtcc ccgtcggccc
     7321 ggttcaccgc ggcagcatcg ccgaggcggg cgacgcgctg gtgccggcgc tgctgggggc
     7381 ggcgggcgcc tcccctgcga ccgccgaggc cgagcgcctc ctcgccgggc acgcgccgaa
     7441 cgaggagaag cacctgttcg ccgtcgccgg cggcacggtc ttcgcccgcc tcccgctcag
     7501 gaccagcctg gtcggccgcg gcgacgacct cgacgagctg atgcgtcgtg agatcgccgc
     7561 gcacatcaag cccggcgacc tcgtggtcat ggccgagaag cccatcgccg cgagccaggg
     7621 tcgcagctac gccttggacg agatccatcc gaccaagctg gccagggtcc tcagcaaggc
     7681 cgtgaccaag accccgcacg gcatcggcct cggcatcccc gagaccatgc agctcgccat
     7741 cgacgaggcc ggcgcgccgc gcatcgtcgc cgccgcggcc gtgtccgccg ccggtaaggc
     7801 cgtcggcaag cgcggcttgt tctacaaggt cgccggcgcc gatgtggagg ccatcgacgg
     7861 ccccacctgg aacacgcttc ctccgcacaa cacccacgcc aagctcgggc cggccgaccc
     7921 ggacggcgtg gccgcacacc tcgcggacgt cctgagcgac gccgtcggcg gtcgcgtcga
     7981 gttcgtcgtc atcgacgcca acgacctcac cgcggccgtg ctcggcgcct cccagggcgc
     8041 cgaccgcgcg ctcgccaggc gcctcatgcg tgacaacccc ctcgggcagg gccacgagca
     8101 gacgccggtc tgcgccctgc gcgcgctcgg cccgctcacc ggcgcagtcc agcagggctg
     8161 acctctccat cgccgtccgc gcgcggcggc ggacgcgcgt tcacctcaat cgggtgatgc
     8221 cccggcttca ccggcgcgac taccctcctc tgcaacacat ccatgagcgt gccgccgggc
     8281 ggcgatgtga aggagggtca cgatggctac ggcgagggac ggcatcagag tggcggatcc
     8341 gggaccgccg ccggtcaggc ttgccgacgg gcctgagccg gtctgcggcg agtgcaggtt
     8401 ccacatcccg cacctcgcga ggccgggcgg ctggtgcgcc tgcgacgcgg cggggctcag
     8461 gtggaggatg gtcgcggcgg gacgcccggt gtgccgcgac ttcgcaccgt ggcccggcaa
     8521 cggcgccgcg gcgtgcgttc ttgcgcagcc gcaggctgtg atcacgccac gtcacccgcg
     8581 cggtggagcc acggtctact gagacgactc gcggcgagtc ggcccgaccg gcgacccgac
     8641 ggcgaccatc tcacctgacg gtcagccgcc gcgcgccggg cttgctctgc gtgttaccgg
     8701 cctggtcggt cgcgaagacc ttccaggtat agcgcccggg ggcgagcctg caggtccagg
     8761 cgtgcgttgc gttctgattg gtcctgacga cccccggctt cagcgtcttg acggtccgcg
     8821 tgcccctgta gatcctgatc cggcaggtcg ctcgtggcgt gacgtcgtcg atgcgatacc
     8881 tgagcgtcgc cctcctgccc tgcctcgccg tggcatcacc gagcgccagc ggcgtggggc
     8941 gtcctgcgtc cacacggacc gtgacccgtc gcgtcgcctc cgtgttgccg gcgtcgtcga
     9001 cggcgcggaa cgccagggcg tgggccgcgt tcccggtcac ctcgcgcgtg gcgccccgga
     9061 cccaatcgcc gccgtccagg ctggactcga cgtaggcgac gccggagcct gcgtcgaggg
     9121 cggagaagga gacggtgacg gcggacgcgt gccaggcgcc gtcgaccccc gtcgtcgtcg
     9181 tcaccggtgc cccggtgtcg acggcgccca ggacgtcgag acgcccgccg gtcgcgcact
     9241 tgccggtgag actcgcgacg gggtccgcac tcgcgagcac ccgggcgatc cgctccgcga
     9301 ccgactcttc cgggtagagt gccgcacaca aggcgatcgt gccggtcacg tggggcgtcg
     9361 ccatcgacgt gccgttcctg gagacgtagc caccggggtc cgtcgaggcc ggtacggtgc
     9421 tccggatgcc gactcccggc gcgacgagat ccaccgaggc ggcgccgtga tcggagaact
     9481 ccgcgagctg gtccgcgccg tcggaggccg ccaccgcgat gatgttcggg ctgtcatagg
     9541 aggccggata gtacggcacg gcgtcgttgt cgtcgccgat cttgtcgccg ccgccgttgc
     9601 cggcggccgc gacgacgacg atgccggcgt cgccggcggt ctcgaccgcc cagcggagaa
     9661 aggcgctcgc ctcgccgttg ccccaggacg cgttgatggc gacgacgttg acgccccgct
     9721 ccttcatcgc gaccgcgtag gtgatgcacg agatggcgtc ggaggtggcg cccgagccgt
     9781 cggcgccgag gaagcggagg gccatgatgc tcgccttcca ggcgacgccg gtcacgccca
     9841 tgccgttgtc gcccaccgcg gccatcgtcc cggcggtgtg cgtcccgtga tcctgcacgt
     9901 ccatagggtc cgacgggtcg gccgcgtcgg gcgcctcggc gattccgtgg acatcgtcga
     9961 cgtagccgtt cgcgtcgtcg tcgagcccgt tgcccgctat ctcgcccggg ttgcgccaca
    10021 tgttggcggc gaggtcgggg tgcgtgtagt ccacgccgct gtcgacactg gccagcacga
    10081 ccgacgagga ccccgtcgtc aggtcccagg cgcgcggcgc ccgtatccgc ggcatacccc
    10141 acagcgccgg aaggtccggg tcgtcgggcg tcgcctcgag gcgcaggagg tagtccggct
    10201 cggcgcggtc gacccggggg tcggcggcca gctcggcgac cagctcggcc gtcgagagcg
    10261 aggccgagcg gaccacagcg gagcggccgc cgcccggcgc ccgcggcagg gacttcgtga
    10321 cgcgggcgcc gtgcgcgagc gcgatccggt ccatgacgcc gacggagaca ccctggtgga
    10381 accggaccag ggcctgcctc ggcacgaagc gggcgccggc cctctcgggg ccgtgcggag
    10441 cgggcgtgac ggcggtcgcc ggcgctgcgt gagccgcgcc ggcgagcagc gggaccgcga
    10501 gccccacgat gatgacgagc agatggtcgc gcaccctgcg catggttcag cctccctcgc
    10561 cgggacgata ccagacgccg cgtgccgacg ggcttaccga ccaacagcta ccgtgcgtga
    10621 ccgccgcccg ccgtcaggtg tataacgagg ctcggccgga gcagcctacg gggaggtcga
    10681 gcggtgagca tcgtcgaggt caaggagctg cgcaagcagt acgacccgcc caagggcgtg
    10741 ctcgcagtcg acggggtgag cttcgacatc gccgagggcg agatcttcag cctcctcggc
    10801 cccaacggcg ccggcaagag caccaccatc tccatgctga gctgtctgct gcggccgacg
    10861 ggcggcgacg cgacggtcgc cggccactcc gtggtcagta acgctcaggc ggtcaaggag
    10921 accatcggcg tcgtgccgca ggacatcgcc ctctacccgg acatgagcgc gcgcgagaac
    10981 ctcgtcttct gggggcgtat gtacgggctc tccggtgaca ccctcaagca acgggtggat
    11041 gaggtgctgg agctcatcac cctcgtcgac cgccagaagg accgcatcga gaagttcagc
    11101 ggcggcatga agcgacgcgt caacatcggc gtcgcgctgc tgcacaagcc gcgggtgctc
    11161 tacctcgacg agccgaccgt ggggatcgac ccgcagagcc ggcggtcgat cctcgacggc
    11221 atcctcgcgc tccgcgacca ggggatgacg atcctctaca cgacgcacta catggccgag
    11281 gcgcaggagc tcagcgaccg catcggcatc gtcgaccagg gcaaaatgat cgccctcggc
    11341 acgcacgagg agctggtcaa gatcgtcggg caggcggctc gcgtgaccgc ccgggtggac
    11401 cactcgctcg aggcgatggc ggagcgctgg cgggaggtcg cgggcgtggc gcaggtcacc
    11461 atcgaggagg acgaggcggt cctgctcacg gatgacgcga acgaggtgct gccgcggctg
    11521 ttcgaggcgg cggccgcggc cggctcgcgg atcacggaga tcgacgtccg cgagcccaac
    11581 ctcgagatgg tgttcctgca cctgaccgga cgcgcgctca gagagtgatc gccgcaggcg
    11641 gatcaccgtc gggtgcaccg cgggtacacc cgacaatgat gtgatgatga agatactcgc
    11701 catcgcctgg aaggacctgc gcagcacggc gcgcaacgtg ccggcgctgg tgatgatgct
    11761 ggccgcgccg ctggcgctcg ccgcgctgct cggcttcgcc ttcggcggcg gcggggggtt
    11821 cgacatcgcc gcgaccaagg tcgtggtggc gaacctggac gggggcggcg ccggggccgg
    11881 ccagaccgcc ggcgccgcca tccagaccgt cctcaccggc gagggtctcg aggacgtgct
    11941 cgagacgagc acccggagct cggccgccgc ggcccggcag gccgtcgacg acggcgaggc
    12001 ggcggtcgcg gtcatcgtgc ccgaggactt cagcgccgtc gtgtacggcg gcgacccggc
    12061 ggcgaggtcc caggtcgaga tctacgagaa cccgacccgc gagatcggcg gctccatcgt
    12121 cgagggcgtg gtcgggcagg tgctcgccga catcaacggc gcgcgggcgg cggccgccgg
    12181 cgccgtcgcg ctcgccgcgg cgggcagcga ccccatcgac ccgcgggtgc tcgccgcacg
    12241 tgccgccgag cagttctccc atgacggcgg cgtctccgcc ggcctgcaga tcggccagcg
    12301 cgctcccagg gtcggcgcga ccgaggagga ggtcagcgtc accgggctca tcctcgccgg
    12361 catgatggtg ttcttcatgt tcttcggcgc ggccaacgtc gcgcgcacga tcctcaccga
    12421 ggaccgcgac ggcacgctcc cgcgcctgtt cacgacgccc acgcggcacg ggaccatcat
    12481 cggcggcaag ttcgtctcca cgttcctcac ggtgctggcg caggcgatcg tcctgctcgt
    12541 cgcggggcgc ctcatcttcg gcatccagtg ggggcccctc gacgcggtcg tgctgctgac
    12601 ggtggtcacg gccggcgtcg ccggcggtct ggcgctgctc gtcatcgcgt tcaccaggaa
    12661 cgcggcgcag gcgggcgcga tcggcgccgg cgtctacctc gtcctcgcgc tgctgggcgg
    12721 caacttcacc ggcacggcgc agaccggcac cacctacgcg ttcatccagc ggttcacccc
    12781 caacggctgg ctcctgcacg ggtgggacac ggccatgcgc ggcggcggcg cggccgacct
    12841 ccgctggcag ctgctcgtgc cgctcggctt cgcggtggcg ttcttcttct tcgccgtgct
    12901 gcgcatgcgg agacggttcg catgaggtgg tcgccgtgaa gctggccgcg gtcctcgccc
    12961 gtaaagacct cctgcagacg gtgcgcgaca agctgtcgtt catcttcatc ctcgtgatgc
    13021 cgctggcgtt cacgctgttc ttcggcctgc tcttcggcgc cggaagcagc gtcgaccagc
    13081 tgccgctcgc cgtgtgggac gcggacgggg gcgccgccgc gacgcagctg gtggcggcgc
    13141 tcgaggagtc gccggtcgtg cgggtcgtgg tcaaggaggg gaacgagttc gagacgtgga
    13201 tggccgacga gcgggccgcg gccggcctcc tggtcccgga gggctacagc gccgccatgg
    13261 ccgccgggga gcgcgccgac ctgacgctcg tctccatcca gggctcgagc ggcgccgcga
    13321 cggtcgccag cgaggtccgc accctggctg gggaacaggt caccgtggag ctcgcctcgc
    13381 gggtcgccgc cgaagtcgtc tggtcgacgc gcagcatgcc ggccggggct caggaggagg
    13441 tcgtcgccga cgcagccgcc caggcccggc ccatcgtcgc gcaggcgctc gcgagcccgg
    13501 ctgtgagcac cgacgtcgtg gaggccggcg tcgccgccgg ccagatcccg agcgggttcg
    13561 tgctcagctc gccgggcatg ctggtgaact tcatcttgtt cagcctgctc acggccggca
    13621 tcgctctcat cgtcgagcgg cagaacggca cgctgcagcg gctcatgacc acccgtctcc
    13681 ggcgctggca gctcatcggc ggcaaggcgg ccgggatgtt cctgctgacg ttcgtgcagc
    13741 aggtgctgct catcgccgtg gcccagatct tcttcggcgt ggactacctg cgcgatccgg
    13801 cggcgctgct cctgatgatg gtctcgctgt cgctcgtggc gagcacgctc gggctgctgc
    13861 tcgcgtctgt gctgaagagc gagcaggcgc tcatcgcgac caccgtcctc gtctcgatgg
    13921 gcatctccgc cctttccggc gcctggttcc cgctcgaggt cacgggagcg gcgttccagt
    13981 ccgtcgggca cctgctgccc acggcctgga tcctcgacgg gctgcgtggg atcgtggtgc
    14041 gcgggttcga cgtcgccgac gtgctgcccg cgctcggcgt ctcggtggcg tgggcggccg
    14101 cgttcttcgc cctcgccgtc tggcgcttcc gcctgagcga ttgacggatc agtctttgga
    14161 cggcttgatg ctggcgtcct tgggcagggc caggccggcc tccacctcga tcgccttgat
    14221 gatcccactc ggcacggggc aggccgggtg cacgaggtgt tccgcggcga gccggagcgt
    14281 caccggtccc tcgccgcggt agctgatctc ccggtacggg tcgacctcgg tgagcgcttc
    14341 cgcgagcttc tcgacgtagc cgcactccgt ctcgaactcg acacgcacgg tgcggccctc
    14401 actggtcgtc ttgacggtgg tgcagaagcc gcagatgccg ctctcgatct cggctgaagc
    14461 catcggccct cctcgtgaca ggttcgttcg gtgaggcaat ggtaccccat gggggtattt
    14521 cctgagcgcg ggacgcgcca gggacggcac aggccttctt acggccaggg cagcacggag
    14581 acggcctgct ccacgagccg cagcagcggc gccgggtaca cgccgaacac cagcaggaac
    14641 agcgtgagca gggccaggac ggtcatggcc acgacgcttc cgtccgcggc cacgc
//
LOCUS       DASUMX010000070         2106 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_450753, whole genome shotgun sequence.
ACCESSION   DASUMX010000070 DASUMX010000000
VERSION     DASUMX010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450753"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..821)
                     /locus_tag="VFZ86_03260"
     CDS             complement(<1..821)
                     /locus_tag="VFZ86_03260"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEX5641340.1"
                     /translation="MIATSSSASALGWAAIALAAGVLAGALVHVLVVHRLRRYGEARG
                     LRWPTAAADSLRGLLEVWGGLVGVVIADPTASLSEGWSVWLERAWIVVAVLSVTVFLT
                     RLAGTLTRSLVASDQSRLPSATIVLNIARFVIWVIGFMVVLNLLGVDIGALLTALGVT
                     GIAVSLGLQDILRNLFTGMQITLTHQYEPGQFVHLQSGEEGEVIDLTWRETRLRTPTG
                     DIAVVPNSVIGNTVVTNYSRGDELYVLAVEFKVVADADLDRVVHVAVEAARATAGA"
     gene            complement(1032..1107)
                     /locus_tag="VFZ86_03265"
     tRNA            complement(1032..1107)
                     /locus_tag="VFZ86_03265"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1072..1074),aa:Gln,seq:ctg)
     gene            complement(1218..>2106)
                     /locus_tag="VFZ86_03270"
     CDS             complement(1218..>2106)
                     /locus_tag="VFZ86_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HEX5641341.1"
                     /translation="LSKRVLLRLVNEGTVRGWDDPRMPTMSALRRRGFPAEGIREFAT
                     MVGVAKADNTVEVEMLEHCARDVLNRAALRRFAVLDPLRVIIENYPEGQTEEMEVPNN
                     PEDPSAGSRRVTFSRELWIERDDFMEEPVRKFFRLAPGREVRLRSAYFVTCNEVVKDD
                     EGRVIELRCTYDPATRGGDAPDGRRPKATLHWLSAAHAVPAEVRLYDHLFASPYPGAD
                     GRDIFEDVSAESETVLRGCFVEPALAEVPVGETVQFERLGYFCPDPDSAPGALVFNRT
                     LTLKDTWAKLQKQGRQNRA"
BASE COUNT          332 a          693 c          781 g          300 t
ORIGIN      
        1 gccccggcgg tggcgcgggc ggcctcgacc gcgacgtgca cgactcggtc gagatcggcg
       61 tcggcgacga ccttgaactc gacggcgagc acgtagagct cgtcgccgcg tgagtagttg
      121 gtcaccacgg tgttgccgat caccgagttc ggcaccacgg cgatgtctcc ggtcggcgtg
      181 cgcagacggg tctcgcgcca ggtgaggtcg atgacctcgc cctcctcgcc gctctgcagg
      241 tgcacgaact ggcccggctc gtactggtgc gtcagggtga tctgcatgcc ggtgaagagg
      301 ttcctcagga tgtcctgcag gcccagggag acggcgatgc cggtgacgcc cagcgccgtg
      361 agcaaggcgc cgatgtcgac gccgaggagg ttgagcacca ccatgaagcc gatgacccag
      421 atcacgaagc gggcgatgtt gaggacgatc gtggcgctcg gcaggcgcga ctggtcggag
      481 gcgacgagac tcctcgtgag cgtgccggcg aggcgcgtca ggaagaccgt gaccgagagc
      541 actgcgacga cgatccaggc gcgctcgagc cagacagacc aaccctcgga gagcgaggcc
      601 gtggggtcgg cgatgacgac gccgaccagg ccgccccaga cctcgagcag cccgcgcagc
      661 gagtccgccg ccgcggtcgg ccagcgcagg ccgcgcgcct cgccgtacct gcgcaggcgg
      721 tgcacgacga ggacgtggac aagggccccg gcgagcacac cggcggcgag ggcgatcgcc
      781 gcccaaccca aggcggaggc cgagctggag gtcgcgatca cgtcaagaca gccgtagtcg
      841 gctgaaccgg ttgatggagc gtgccgctgt cctgaacctc tcggtcatgt ccgttcaggt
      901 tcggcgcgga gcacctgccc ccttgtggcg gtcggcttcc ggggcgctgg gggtcgccgg
      961 acagcaccag agactcgctg gtcgaccacc gggcgacgcc tgcggacgcg tgcggccggc
     1021 aggcaaagaa atggctggcg aggaaggact cgaaccttca atcccctgat ccagagtcag
     1081 gtgccttgcc aattaggcca ctcgccagcg gcgcagagag tgtaccccgg ggcggctcat
     1141 cgctcaaccg tcggagcggc cggaagcact gaggaatgcg gcttgagcgg gcgctttgcg
     1201 ccgcgcggcg gcagagctca ggctctgttc tgccgcccct gcttctgcag cttggcccag
     1261 gtgtccttga gcgtgagcgt gcggttgaag acgagcgcgc ccggcgccga gtccgggtcc
     1321 gggcagaagt agccgaggcg ttcgaactgc acggtctcgc cgacgggcac ctcggcgagc
     1381 gccggttcca cgaagcagcc gcggagcacc gtctcggact cggcgctcac gtcctcgaag
     1441 atgtcgcggc cgtcggcgcc ggggtacggg ctggcgaaga ggtggtcgta caggcggact
     1501 tcggcgggca cggcgtgcgc cgccgagagc cagtgaaggg tggccttggg gcggcggccg
     1561 tcgggcgcgt cgccgccgcg cgtcgccggg tcgtaggtgc agcgcagctc gatgacccgc
     1621 ccctcgtcgt ccttgaccac ctcgttgcag gtgacgaagt aggctgagcg cagccgtacc
     1681 tcgcgcccgg gcgccaggcg gaagaacttg cgcaccggct cctccatgaa gtcgtcgcgc
     1741 tcgatccaga gctcgcggga gaaggtcacg cggcggctgc ccgcggacgg gtcctccggg
     1801 ttgttgggga cctccatctc ctcggtctgg ccttcggggt agttctcgat gatcacgcga
     1861 agcgggtcga gcacggcgaa gcggcgcagc gcggcacggt tgagcacgtc gcgcgcgcag
     1921 tgttcgagca tctccacctc gacggtgttg tctgccttgg cgacaccgac catggtggcg
     1981 aactcgcgga tgccctccgc gggaaagcca cggcggcgga gggcggacat cgtaggcatg
     2041 cgcgggtcgt cccagccacg caccgtgccc tcgttgacga ggcgcagaag cacgcgcttg
     2101 gacagc
//
LOCUS       DASUMX010000071         4593 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1744950, whole genome shotgun sequence.
ACCESSION   DASUMX010000071 DASUMX010000000
VERSION     DASUMX010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4593
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1744950"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VFZ86_03275"
     CDS             <1..232
                     /locus_tag="VFZ86_03275"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEX5641342.1"
                     /translation="PNLTVVRVLSQPSEAWTGERGYITADVLRRHLPEKLLVHFQYFI
                     CGPGVLMDAMEAALPALGVPVDRVHTERFEMV"
     gene            242..847
                     /locus_tag="VFZ86_03280"
     CDS             242..847
                     /locus_tag="VFZ86_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641343.1"
                     /translation="MTHKVVSAVVGLIVLLFVAAALAFAWSVAARAPEPAPAQAGPAA
                     VPHGVEDGAADCARCHRVSEGSLPLTHREFPQGACTNCHALRRPVAIPHGVSMGEDRC
                     VLCHGQAAAPLGMPNGHREFDGVSCAFCHPQDERRATVQPPPAGESAKQAPPVRHAVT
                     GIFKDCLTCHEVDGRPPMPDSHLGFGGDTCRFVCHFRAGGR"
     gene            900..1667
                     /gene="hdhA"
                     /locus_tag="VFZ86_03285"
     CDS             900..1667
                     /gene="hdhA"
                     /locus_tag="VFZ86_03285"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006668008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HEX5641344.1"
                     /translation="MSIDQLLDLRGQTAIVTGGGAGIGRAIAERLAQAGAAVVVGDLD
                     AVTAQEVADGIAAAGGSAAAAACNVTLEQDRAAVVDLAVARFDGPTILVNNAGGGGPQ
                     PFDMPLEKFEWAYELNVFSGFRLCQLCAPLMAEKGHGAIVNISSMAGENTNVHMTSYS
                     SSKAAVNHLTRNMAFDLGPRGIRVNAVAPGAIKTHALSTVLTPEIERQMLKHTPLGRL
                     GEAEDIASVVLFLVAPMSSWVSGQVITVSGGGTQELE"
     gene            1732..2394
                     /locus_tag="VFZ86_03290"
     CDS             1732..2394
                     /locus_tag="VFZ86_03290"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HEX5641345.1"
                     /translation="MTPERTQGGALTSLLPTTRLEAFSDGVFAIAITLLVLELHVPTD
                     PAELLHELAAEWTGYLGYFVSFAFIGGVWMAHSTLTRFIKAADQVMMGLNLVLLLFVS
                     FLPFTTSLLANHLDDSGAHIAVVVFGINLTLASLMVNVMFGYASRAEGVAADDAADDE
                     LKAFDKQRRAAVILQGVATMIGLLVPFVAVVVYLVISLLVLVDPIWRAERRRRAAKRG
                     AA"
     gene            2503..3870
                     /locus_tag="VFZ86_03295"
     CDS             2503..3870
                     /locus_tag="VFZ86_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009995277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 domain-containing protein"
                     /protein_id="HEX5641346.1"
                     /translation="MRPTAEESTQTGRDARAIVSRGDLGLYEPAAARRDPVEILVEQG
                     AGRQADLLPIRYGRMAASPFAFLRGAAAVMAADVAPSPVTGLTVQAIGDAHISNFGVF
                     ATPERRLAFDVNDFDETLRAPWEWDVKRLVASVVVAAAENGAKRSAAAAAAERAATAY
                     RRSMHGFAAQPTLETWYSHLDVGNLVEREGSKQKRERDRKSLEKIRGRGSEQVVGKLT
                     ERAGDSLRFKSIPPLVTPLRDLVKEEETEAAREAVLRAFAEYRKSLAPERRQLLDRFQ
                     LSDLARKVVGVGSVGTRCLIALFVGHSDDDVLVLQFKEAGASVLEPYAGRSPYRHEGA
                     RVVNGQRAVQVAGDVFLGWSTLEKARHYYWRQLRDMKASAEIGTLGEKQFDDYVAACA
                     WCLAHAHARTGDPIAIAAYLGSGAAFDKAMAAFGAAYAEQTRTDWKALTAAIADGRVQ
                     ATRGV"
     gene            3903..4088
                     /locus_tag="VFZ86_03300"
     CDS             3903..4088
                     /locus_tag="VFZ86_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641347.1"
                     /translation="MEWWYGLILAIVDIAFAIFCGLFAQRKGYSVFWFTVLGLLFNVI
                     TFVVIAVLPDKYQTGDW"
     gene            4302..>4593
                     /locus_tag="VFZ86_03305"
     CDS             4302..>4593
                     /locus_tag="VFZ86_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641348.1"
                     /translation="MGFMDDAKKMADDAAAKAKQAAADAKEKSGPALDKAKATAGELT
                     DKAKATTADLGEKAKPTTDKAKSEAAEIIDKAKKEALDLIERAKKEGPELLQK"
BASE COUNT          736 a         1616 c         1591 g          650 t
ORIGIN      
        1 gccgaatctc accgtcgtgc gcgtgctctc gcagccgtcc gaagcatgga ccggcgagcg
       61 cggctacatc accgccgacg tactgcggag gcacctcccc gagaagctgc tcgtgcactt
      121 ccagtacttc atctgcggcc caggcgtgct gatggacgct atggaggccg ccctgccggc
      181 gctcggcgtc cccgtggacc gggtccacac cgagcgcttc gagatggtgt gaggtgaagt
      241 cgtgacgcac aaggtcgtct ccgccgtcgt cgggctgatc gtgctgctct tcgtcgctgc
      301 ggccctggcc ttcgcctgga gcgtcgccgc gcgagccccg gagccggcgc cggcccaagc
      361 cggccccgcc gccgtgccgc acggcgtcga ggacggcgcc gcggactgcg cgcgctgcca
      421 tcgggtctcc gaagggtccc tgccgctcac ccaccgagag ttcccgcagg gcgcatgcac
      481 caactgccac gccctgcgga ggcccgtcgc tatccctcac ggggtgtcca tgggcgagga
      541 ccgctgcgtg ctctgccacg gccaggccgc cgcgccgctc ggcatgccta acggtcacag
      601 ggagttcgac ggcgtgtcct gcgcgttctg ccaccctcag gacgagcggc gggccaccgt
      661 gcagccgccg cccgccgggg agtcggccaa gcaggcgccg ccggtgcgcc acgccgtgac
      721 cggcatcttc aaggactgcc tcacctgcca cgaggtggac ggcaggcctc ccatgccgga
      781 cagccatctc ggcttcgggg gcgacacctg ccgcttcgtc tgtcacttcc gcgcgggagg
      841 gcgctgagac gcaactgccg ccacgcctcc gcccgcccca ggcatcacaa ggagcctcca
      901 tgtccatcga ccagttgctc gacctgcggg gtcaaaccgc catcgtcacc ggaggcggcg
      961 ccggcatcgg cagagccatc gccgagcggc tggctcaggc cggcgcggcc gtcgtcgtcg
     1021 gcgacctgga cgccgtgacg gcgcaggagg tcgcagacgg catcgccgca gccggcggca
     1081 gcgctgctgc ggccgcctgc aacgtgacgc tcgaacagga ccgggccgcc gtggtcgacc
     1141 tcgcggtcgc gcggttcgac ggtcccacca tcctcgtcaa caacgcgggt ggtggtggcc
     1201 cgcagccgtt cgacatgccc ttggagaagt tcgagtgggc ctacgagctc aacgtgttct
     1261 ccggcttccg cctctgccag ctgtgcgccc cgctcatggc ggagaagggt cacggggcga
     1321 tcgtcaacat cagctcgatg gccggcgaga acacgaacgt gcacatgacc tcctactcct
     1381 cctccaaggc ggccgtgaac cacctcacgc gcaacatggc gttcgacctc ggcccgcggg
     1441 ggatccgcgt caacgccgtc gctcccggcg ccatcaagac gcacgccctc tccacggtcc
     1501 tgactcccga gatcgagcgc cagatgctca agcacacgcc tctggggcgt ctcggggagg
     1561 cggaggacat cgccagcgtg gtcctgttcc tcgtcgcgcc aatgtcgtcc tgggtgagcg
     1621 gacaggtcat caccgtgagc ggaggcggca cgcaggaact cgagtaggtt tgaaggacca
     1681 tgcggtctgg aaagattgcc gccatctgtc tgagcgtcag aaaggagacc agtgaccccc
     1741 gaacgcacac agggcggagc gctcaccagc ctgctcccca ccacgaggct ggaggcgttc
     1801 tcggatggcg tgttcgccat cgcgatcacc ctgctcgtgc tcgaactgca cgtcccgacg
     1861 gacccggcgg agctcctcca cgagctcgcc gcggagtgga ccggctacct tggctacttc
     1921 gtcagcttcg cgttcatcgg cggcgtctgg atggcgcaca gcaccctgac gcggttcatc
     1981 aaggctgcgg accaggtcat gatgggcctc aacctggtcc tcctcctgtt cgtctcgttc
     2041 ctaccgttca cgacgagcct gctcgccaac cacctcgacg acagcggcgc gcacatcgcc
     2101 gtcgtcgtct tcggcatcaa cctgacgctg gcgtcgctca tggtcaacgt gatgttcggc
     2161 tacgcgagtc gcgccgaggg cgtggccgcg gacgacgcgg cagacgacga gctcaaggcg
     2221 ttcgacaagc agcgacgcgc cgccgtcatc ctgcagggcg tggcgacgat gatcgggctt
     2281 ctcgtcccct tcgtcgccgt cgtcgtctac ctcgtcatct cgctcctcgt gctcgtcgac
     2341 ccgatctggc gcgcggagcg ccggcggcgg gcggcgaaga ggggcgcggc gtgaccgcgc
     2401 tcgcgtccgg gggcgcctgc gaccggtcga gcttgaagcc ctcgaggcgc ccggcgcacg
     2461 cgcgaagttc caacacgccc gcccacacga agaggagtga tgatgcgccc cacagctgag
     2521 gagtccacgc agaccggcag agacgcacgg gcgatcgtga gccgcggaga tctggggctc
     2581 tacgagcccg ccgcggcgcg gcgcgaccct gtcgagatcc tcgtggagca gggagccggc
     2641 cggcaggccg acctgctccc catccgctac ggccgcatgg cggcgtcgcc gttcgcgttc
     2701 ttgcgcggcg ccgccgccgt catggccgcc gacgtcgcgc cgtccccggt caccggcctg
     2761 accgtgcagg ccataggcga cgcccacatc agcaacttcg gcgtgttcgc gacgccggag
     2821 cggcgacttg ccttcgacgt caacgacttc gacgagaccc tgcgcgcccc atgggagtgg
     2881 gacgtcaagc ggctggtggc cagcgtcgtg gtcgccgccg cggagaacgg cgccaagcgc
     2941 tccgccgcgg ccgcagccgc cgagcgcgcg gcgaccgcct accgcaggtc catgcacggc
     3001 ttcgccgccc agccgacgct cgagacctgg tactcgcacc tggacgtcgg caacctcgtg
     3061 gaacgcgagg gatcgaagca gaagcgggag cgcgaccgca agtcgctgga gaagatccgc
     3121 ggtcgcggca gcgagcaggt cgtgggcaag ctgaccgagc gcgccggcga cagtctgcgg
     3181 ttcaagagca tcccgccgct cgtcaccccg ctccgcgacc tcgtcaagga ggaagagacg
     3241 gaggccgcgc gcgaggccgt gctgcgcgcc ttcgccgagt accgcaagag cctcgcgccc
     3301 gagaggcgcc agttgctgga ccgcttccag ctctccgacc tggcgcgcaa agtcgtcggg
     3361 gtcggcagcg tgggcacgcg ctgcctgatc gcgctgttcg tggggcacag cgacgacgat
     3421 gtgctggtgc tccagttcaa ggaggccggc gcgtcggtgc tcgagcccta cgcgggccgc
     3481 agcccctacc ggcacgaggg cgcgcgcgtg gtcaacggcc agcgcgccgt gcaggtcgcc
     3541 ggtgacgtgt tcctcggctg gagcacgctg gagaaggcgc gccactacta ctggcggcag
     3601 ctgcgcgaca tgaaagcctc ggcggagatc gggacgctcg gggagaagca gttcgacgac
     3661 tacgtggcgg cgtgcgcctg gtgcctcgcg cacgcgcacg cccgcacggg ggaccccatc
     3721 gccatcgccg cctacctcgg atccggcgcc gcgttcgaca aggcgatggc cgctttcggc
     3781 gcggcctacg ccgagcagac ccggaccgac tggaaggcac tgaccgcggc catcgccgac
     3841 gggcgcgtcc aagccacgag gggtgtgtga accggggcga cccgtcatcc aaggaggtac
     3901 agatggagtg gtggtacggc ctcatcctgg cgatcgtcga catcgcgttc gcgatcttct
     3961 gtggcctgtt cgcccagcga aagggctaca gcgtcttctg gttcaccgtg ctcggcctgc
     4021 tgttcaacgt gatcacgttc gtcgtcatcg cggtgctccc ggacaagtat cagaccggcg
     4081 actggtgacg gccgtgcgtt gctgagggcg gtggtccacg accgctggcc tgagcaggcc
     4141 aaggacacgg tcccgccacg gagaggcatg cgggatgacg gagtcacgtc ggttccccgg
     4201 gcgacggcgt caaggcccga gttgacgcag aggaggcagg agcagggctc tggggtaaag
     4261 tgaatgcgcg acgcaccgtc accacacgga gggagcggca gatgggattc atggacgacg
     4321 ccaagaagat ggccgacgac gctgcggcga aggcgaagca ggccgcggca gacgccaagg
     4381 agaagtccgg cccggcgctg gacaaggcca aggcgacggc cggcgagttg accgacaagg
     4441 ccaaggcgac gaccgcggat ctcggcgaga aggccaagcc cacgaccgac aaggccaagt
     4501 ccgaggcggc ggagatcatc gacaaggcca agaaggaggc cctggacctg atcgagcggg
     4561 ccaagaagga ggggccggag ctgctccaga agg
//
LOCUS       DASUMX010000072         8018 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_307566, whole genome shotgun sequence.
ACCESSION   DASUMX010000072 DASUMX010000000
VERSION     DASUMX010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 8018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8018
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307566"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /locus_tag="VFZ86_03310"
     CDS             <1..461
                     /locus_tag="VFZ86_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019735375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX5641349.1"
                     /translation="SHQGDPTTMDVARRVDAYAGRMIHRAPWLLLLADVMLGEVALEQ
                     GDLIEARRWSARATATVKDYSDAGMLGRRSRSLAAAIEERMYGESVTPAEQRVLDLLP
                     THLTEKEMAARLYLSTATVKTHRRSIYRKLGVNTRGEAVARARGLGLLPR"
     gene            547..2595
                     /gene="thrS"
                     /locus_tag="VFZ86_03315"
     CDS             547..2595
                     /gene="thrS"
                     /locus_tag="VFZ86_03315"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEX5641350.1"
                     /translation="MTLHVTLPDGSPLELDDGASVRDAAAAIGPRLAKAAIAGRVTAA
                     DDPDVTLLVDAAAPLHDGDRLDIVTLKGEDPDAVDIQRHTASHVMAQAIVRLFPGAKY
                     AIGPTIENGFYYDLQLPEPIAEADLARIEKEMGKVVSQNLPVERYELPVDEALAVFGA
                     GEPGTGASGLPLGLDQPFKIELIEDLVTAAEATGDEVPTISVYRQGDFVDLCRGPHLP
                     STNKLGNGTFKLTSLAGAYWRGDEKNPMLTRVYGTAFPSKEELAAHLEALELARQRDH
                     RRIGRDLDLFSFHEEGPGFPFFHPKGMRLWNAMIDYWRAEHVRAGYEEISTPVILRRE
                     LWERSGHWDNYKENMYFTEIDEQPYAVKPMNCPGGLLIYKSRRRSYRDLPLRMAELGR
                     VHRHEMSGVLHGLFRVRYFTQDDAHIYCTPEQLEDEVRGVLHFMLHIYAAFGFSDVKI
                     ELSTKPEKSIGSDEVWELAEGVLARVLEGEGTDYVLNPGDGAFYGPKIDFHIRDVMGR
                     TWQCGTIQVDFAMPERLDITYTGADNEEHRPVMIHRALLGSIERFMGILLEHYGGNLP
                     TWLAPEQALVLPIADRHGDYAHEVLRALRAAGAGSEAEAPVTIHAGVDDRGESIGKRI
                     RDAQLQKVPYILVVGDREVDQGAVGVRERGEDKGARPLAEFVDDLVAEVRERRLPGQG
                     "
     gene            2599..2817
                     /locus_tag="VFZ86_03320"
     CDS             2599..2817
                     /locus_tag="VFZ86_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641351.1"
                     /translation="MAREPQQTGDACLRCGRPLYTFGVQEFRLGGTTGGWKLLFGEWA
                     ELGEDMIGLEVLACRGCGKVEMRIPDQG"
     gene            2898..4349
                     /locus_tag="VFZ86_03325"
     CDS             2898..4349
                     /locus_tag="VFZ86_03325"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX5641352.1"
                     /translation="MRRHADGGRQPAAPAVGARSWWLVEALRADPGAVCPALTGDTTA
                     DVCVVGGGFAGLWAAYELGERDPSLDIAVLEADIVGAGGSGANGGFFSPSWAQLSSLC
                     ASLGEEGGVAYAAALAAVVDELDGWIARHGARVDHWHEGILYARAGDWQPGPDDETFR
                     LLERHGYADRLRRVDAGAARAVADSPRFVGGVITPDLTVLQPGKLARELRRVLLERGV
                     RIFEHTPMVRVEDGRRPRVVTPGGAVSAGSVVLAHGAWAARERHFRRAFAVCTDFMVV
                     TEPVPDLIERIGWTSHMGVADLREMLYYLRRTADDRIAIGGGAMGIVCGARIRGRALT
                     SPRLAAAAAHGLLWLFPQLEGVRFDAAWSGPMDITGPALPFFESAPGGRVHAGLGFSG
                     HGLTGTRLGGKILASLVLGADDEWSRMAVVGPPLAQLPPEPLRWPLVQSVSLAYEASD
                     RAHEQGSDPGLLPRAVIAGYGRYGALNSATGRD"
     gene            4480..4935
                     /locus_tag="VFZ86_03330"
     CDS             4480..4935
                     /locus_tag="VFZ86_03330"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX5641353.1"
                     /translation="MSDTYTADRITEAARRLDVAMSTLNAAFARDVGVSVPELLALEN
                     LENEGGLGPSELARRLQLSTGAVTALVDRLEASGHAARAAHPSDRRRIVVTRTAKASD
                     ALANEAAPLEFEIRRLAEGLSDDEREVVARFLDAFISIVERAAAEACAS"
     gene            4964..5776
                     /gene="heR"
                     /locus_tag="VFZ86_03335"
     CDS             4964..5776
                     /gene="heR"
                     /locus_tag="VFZ86_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010542476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heliorhodopsin HeR"
                     /protein_id="HEX5641354.1"
                     /translation="MSSTPTTAGSPEVSSRAVTPELERRLGRLRFWNIGVGLVLAVEA
                     VAIALLTNGFTLPVTATYMSGPPGTPAELTTLFDVPLGWGIFAFVAISALALFTIASP
                     GVFSWYKRNLLQDRNYGRWIEYFFSSSLMIVLISMITGVSDIAALLAIFGVNASMILF
                     GLLMEKYETPGKPSWLSFWFGTFAGIIPWIIVAIYVLTPGVDGPAPPGFVYGIVISLF
                     LFFNVFAINMWLQYHKVGKWREYLFGEKAYIILSLTAKSLLAWWVFSAVLAS"
     gene            5853..6398
                     /locus_tag="VFZ86_03340"
     CDS             5853..6398
                     /locus_tag="VFZ86_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX5641355.1"
                     /translation="MPAVSAGLLLYRRVPDPETGARRVEVLLVHPGGPYWARKDAGAW
                     SIPKGMAEPDELGPEPATATAAKARNRAAGADLLAVARRETREETGLEPEGPFRPLGG
                     VKAHGHKLIFAWAVEHDCDAGAITSNTFTMEWPPRSGTQAEFPEVDRAAWFTLDAARE
                     AIVPVQRRFVDDLAVVAAAGA"
     gene            complement(6395..7228)
                     /locus_tag="VFZ86_03345"
     CDS             complement(6395..7228)
                     /locus_tag="VFZ86_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641356.1"
                     /translation="MVHVAADAVGAAWLDGSRAAMAGFLVGLLATFLFVRLNTRLIRA
                     QVSWWFHDIESEGGTHVHHMVIGVVLMVTVGVLLIALAPSGLAAQVLALLFGVGVALT
                     LDEFALILNLQDVYWRKEGRLSVDAVIIVVAAAALFVVGIDPFEDLEQPGRSGDLFRV
                     VVAAYIVVVIVPVLLCLLKGKVWTGVIGFFVPIVAIVGAIRLARLNSPWARRRYRTRP
                     KKLSRAERREARLRAHTLAWRDAFFDLIAGKPHLPSLQPVGAQPDPADPPDDEAAGGR
                     S"
     gene            7365..>8018
                     /locus_tag="VFZ86_03350"
     CDS             7365..>8018
                     /locus_tag="VFZ86_03350"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5641357.1"
                     /translation="MGHNVPSLVKLVGAQGEGWRAFIGSAERGESWSAPGVSVGIAGE
                     AAHEVNLVVAYGPDGVGDGIAAAAAVLRRRGLPGAVYAASPVAAETAEAAAALGFTAS
                     APLPLMCAHAGDVVSVEATHETVRVAGVEGVLAAGDVLADAFDLPVDWCQRLLGVGFA
                     ARADAAAFLALHDGRPVAVAGCAQIGDIAGLYAVGTRHSHRRRGAAAAAVSAAIGHHL
                     "
BASE COUNT         1154 a         2897 c         2863 g         1104 t
ORIGIN      
        1 tctcccatca aggagacccc acgaccatgg acgtcgcccg ccgtgtggac gcctacgccg
       61 ggcgcatgat ccatcgcgcg ccttggctcc tgctgctggc cgatgtgatg ctaggtgagg
      121 tcgccctcga gcagggggat ctcatcgagg cccgccgctg gtcggcgcgg gcaactgcga
      181 cggtgaagga ctactcggat gccgggatgc tcgggcgccg gtcacggtcg ctggcggcgg
      241 cgatcgagga gcggatgtac ggcgagtcgg tgacccccgc cgagcagcgg gtgctcgacc
      301 tgctgccgac gcacctgacc gagaaggaga tggccgccag actgtacctc tccacggcga
      361 cggtgaagac gcaccggcgc tcgatctatc gcaagctcgg cgtgaacacc cgcggcgagg
      421 cggtggcgcg tgcacgcggg ctgggcctgc tgccccggtg acgcccgctt cgtttttgct
      481 agactcggcc ggtcaacagc ccccgcaagg tgcgcgggcg cgcaagtcga gaaaggacca
      541 ccccgcatga ccctgcacgt caccctcccc gacggatcac ccttggagct cgacgacggc
      601 gccagcgtgc gcgacgccgc ggcggcgatc ggcccgcgcc tggcgaaggc cgccatcgcg
      661 ggccgcgtga ccgccgccga cgacccggac gtgaccctcc tggtcgacgc ggccgcgccg
      721 ctgcacgacg gcgaccgcct cgacatcgtc acgctcaagg gcgaggaccc cgatgccgtc
      781 gacatccagc ggcacaccgc ctcgcacgtc atggcgcagg cgatcgtgcg cctcttcccg
      841 ggcgccaagt acgccatcgg cccgaccatc gagaacggct tctactacga cctgcagctg
      901 ccggagccga tcgcggaggc cgacctggcc cgcatcgaga aggagatggg caaggtcgtc
      961 agccagaacc tgccggtgga gcggtacgag ctgccggtgg acgaggcgct cgcggtgttc
     1021 ggggccggcg agcccggcac gggcgcctcc ggcctgcccc tcgggctcga ccagcccttc
     1081 aagatcgagc tcatcgagga cctcgtgacc gccgccgagg cgaccgggga cgaggtcccg
     1141 accatcagcg tctaccgcca gggcgacttc gtcgacctct gccgcggccc gcacctgccg
     1201 agcaccaaca agctcggcaa cgggaccttc aagctcacca gcctcgccgg cgcctactgg
     1261 cgtggcgacg agaagaaccc gatgctcacc cgcgtctacg gcaccgcctt cccgagcaag
     1321 gaggagctgg cggcgcacct cgaggcgctc gagctcgccc gccagcgcga ccaccggcgc
     1381 atcggccggg acctcgacct cttcagcttc cacgaggagg gccccggctt cccgttcttc
     1441 caccccaagg gcatgcgcct gtggaacgcg atgatcgact actggcgcgc cgagcacgtg
     1501 cgcgccggct acgaggagat cagcacgccg gtgatcctgc gccgtgagct ctgggagcgc
     1561 agcggccact gggacaacta caaggagaac atgtacttca ccgagatcga cgagcagccg
     1621 tacgcggtga agcccatgaa ctgccccggc ggcctgctca tctacaagag ccgccggcgc
     1681 tcctaccgtg acctgccgct gcgcatggcc gagctcggcc gtgtgcaccg ccacgagatg
     1741 agcggcgtcc tgcacggcct cttccgcgtg cgctacttca cccaggacga cgcgcacatc
     1801 tactgcaccc cggagcagct cgaggacgag gtccggggcg tgctccactt catgctgcac
     1861 atctacgccg ccttcggctt cagcgacgtc aagatcgagc tgagcaccaa gccggagaag
     1921 agcatcggct cggacgaggt ctgggagctc gccgagggcg tcctcgcgcg ggtgctcgag
     1981 ggcgagggca ccgactacgt gctcaacccc ggagacggcg ccttctacgg ccccaagatc
     2041 gacttccaca tccgcgacgt catgggccgc acctggcagt gcggcaccat ccaggtcgac
     2101 ttcgccatgc ccgagcgcct ggacatcacc tacaccggcg ccgacaacga ggagcaccgg
     2161 ccggtcatga tccaccgcgc gctgctcggg agcatcgagc gcttcatggg catcctgctc
     2221 gagcactacg gcggcaacct gccgacctgg ctcgcgccgg agcaggcgct ggtgctgccg
     2281 atcgccgacc gccacggcga ctacgcccac gaggtgctgc gcgcgctgcg cgccgccggc
     2341 gcggggagcg aggcggaggc gccggtcacg atccacgccg gcgtcgacga ccgcggcgag
     2401 agcatcggca agcgcatccg cgacgcgcag ctgcagaagg tgccctacat cctcgtggtc
     2461 ggcgaccgcg aggtggacca gggcgccgtc ggcgtgcgcg agcgcggcga ggacaagggc
     2521 gcgcggccgc tcgccgagtt cgtcgacgac ctggtcgccg aggtccgcga gcggcgcctg
     2581 cccggacagg gctgagcgat ggcgcgtgag ccgcagcaga ccggcgacgc ctgtctgcgc
     2641 tgcggccggc cgctgtacac gttcggcgtg caggagtttc gcctgggagg cacgaccggg
     2701 ggctggaagc tgctcttcgg cgagtgggcc gagctcggcg aggacatgat aggactcgag
     2761 gtcctcgcct gccgcggctg cggcaaggtc gagatgcgca tccccgatca gggttgacga
     2821 agcttcgccg acatcctgcc gttgccgtac ccgcggtcgg gcggcgacgt cactccacaa
     2881 cgtcaccgga ggaccagatg agacgacacg cggacggcgg gcggcagccg gcggcgcccg
     2941 cggtgggcgc gcgcagctgg tggctggtgg aggcgctgcg cgccgacccc ggcgcggtgt
     3001 gcccggccct gaccggcgac acgaccgccg acgtgtgcgt ggtcggcggc ggcttcgccg
     3061 gcctctgggc cgcctacgag ctgggcgagc gtgacccaag cctcgacatc gcggttctcg
     3121 aggccgacat cgtcggcgcc ggcggcagcg gggccaacgg cggcttcttc tcgccctcct
     3181 gggcgcagct gtcgagcctg tgtgcgtcgc tcggcgagga gggcggggtg gcctacgcgg
     3241 ccgccctcgc cgccgtggtc gacgagctcg acggctggat cgcgcgtcac ggcgctcgcg
     3301 tcgaccactg gcacgagggc atcctgtacg cccgggccgg agactggcag ccggggcccg
     3361 acgacgagac gttccggctg ctcgagcggc acggctacgc cgaccggctg cggcgcgtgg
     3421 acgccggcgc ggcgagggcg gtcgcggatt ccccgcgctt cgtcggcggc gtgatcacgc
     3481 ccgacctcac ggtgctccag ccgggcaagc tcgcccgcga gctgcggcgc gtgctgctgg
     3541 agcgcggcgt gcgcatcttc gagcacacgc ccatggtgcg cgtggaggac gggcgccggc
     3601 cccgcgtcgt cacgccgggc ggggcggtgt cggccggcag cgtggtgctc gcgcacgggg
     3661 cgtgggcggc gcgggagcgg cacttccgcc gcgccttcgc cgtgtgcacc gacttcatgg
     3721 tggtgaccga gccggtcccg gacctgatcg agcgcatcgg ctggacctcg cacatgggcg
     3781 tcgccgacct gcgcgagatg ctgtactacc tgcgtcgcac cgccgacgac cgcatcgcca
     3841 tcggcggcgg cgcgatgggc atcgtgtgcg gcgcgcgcat ccgcggccgg gcgctcacgt
     3901 cgccgcggct ggcggcagct gccgcgcacg gcctgctctg gctgttcccc cagctcgagg
     3961 gcgtgcgctt cgacgccgcc tggagcggcc cgatggacat caccggcccg gcgctgccct
     4021 tcttcgagtc ggcgcctggt ggccgagtcc acgccgggct cggcttctcc gggcacgggc
     4081 tcaccgggac gcggctcggc ggcaagatcc tcgcctcgct ggtgctcggc gccgacgacg
     4141 agtggagccg catggcggtc gtcggcccac cgctcgcgca actgccgccg gagccgctcc
     4201 gctggccgct ggtgcagtcg gtgagcctgg cctacgaggc cagcgaccgc gcccacgagc
     4261 aggggagcga tccgggcctg ttgccgcgcg ccgtcatcgc cgggtacggc cgctacggcg
     4321 cgctcaacag cgccaccgga agggactgaa gcggtcagga ggagcgcgcg tccttcgctc
     4381 acggggcggt gaagccgcgg gagtccggcc cgtcaggata gaaatctcat ctcatgggta
     4441 tcttcattgt cgagatactt atcgacgaaa gagacgctcg tgagcgacac ctacacagcg
     4501 gaccggatca ccgaggcggc gcggcggctg gacgtcgcca tgtccaccct caatgccgcc
     4561 ttcgcgcgcg acgtgggcgt cagcgtcccg gagctgctcg cccttgagaa cctcgagaac
     4621 gagggtggcc tcggcccgag cgagctcgcc cgccggctcc agctctcgac gggggccgtc
     4681 acggctctcg tcgaccggct ggaggcgagc ggtcacgcgg ctcgcgccgc tcatcccagc
     4741 gaccgtcgca ggatcgtcgt gacccgtacg gcgaaagcca gcgacgcgct ggccaacgaa
     4801 gcggctcctc tggagttcga gatccggcgt cttgcggaag gcctgagcga cgacgagcgc
     4861 gaggtcgtcg cccggttcct ggacgcgttc atcagcatcg tcgagcgggc ggcggccgag
     4921 gcgtgtgcct catgaccgcc cgaccacgcg aaaggagcga acgatgagtt ccacccctac
     4981 caccgccggc agcccggagg tttcctcacg cgccgtcacg cccgagctgg aacgccgcct
     5041 cggacgcctg cgcttctgga acatcggcgt cgggctcgtg ctcgccgtgg aggcagtcgc
     5101 catcgccctc ctcaccaacg ggttcaccct gccggtgacg gcgacctaca tgagcggtcc
     5161 acccggcacc ccggcggagc tcaccacgct gttcgacgtg ccgctcggct ggggcatctt
     5221 cgccttcgtg gcgatctccg cgctggcgct gttcaccatc gcttcacccg gcgtcttcag
     5281 ctggtacaag cgcaacctgt tgcaggaccg taactacggg cgctggatcg agtacttctt
     5341 cagctccagc ctcatgatcg tgctcatcag catgatcacc ggcgtcagcg acatcgccgc
     5401 gctgctggcg atcttcggtg tcaacgcgag catgatcctc ttcggcctgc tcatggagaa
     5461 gtacgagacg ccgggcaagc cgagctggct gtctttctgg ttcggcacgt tcgccggcat
     5521 catcccgtgg atcatcgtgg ccatctacgt gctcaccccg ggggtcgacg gcccggcgcc
     5581 gcccggcttc gtctacggga tcgtcatcag cctcttcctc ttcttcaacg tgttcgccat
     5641 caacatgtgg ctgcagtacc acaaggtggg caagtggcgg gagtacctgt tcggtgagaa
     5701 ggcctacatc atcctgagcc tgacggccaa gtcgctgctg gcgtggtggg tgttctccgc
     5761 cgtgctggcc agctgagacc cgtgtgaccg gcgggggccg acggcgccga cggcgccgcc
     5821 ggcccccgcg ccgtacgtag tatcctcgag ccatgccggc cgtcagcgca ggactgctgc
     5881 tctaccgccg ggtcccggac ccggagaccg gcgcgcgccg ggtcgaggtg ctgctggtgc
     5941 accccggcgg cccgtactgg gccagaaagg acgccggggc gtggagcatc cccaagggga
     6001 tggccgagcc ggacgagctc ggcccggagc ctgccaccgc gacggcggcc aaggcgcgca
     6061 accgggcggc gggcgccgat ctgctggccg tcgcccgccg cgagacgcgt gaggagacgg
     6121 ggctggagcc ggagggaccc ttccgtcccc tgggcggggt caaggcgcac ggtcacaagc
     6181 tcatcttcgc ctgggcggtg gagcacgact gcgacgccgg ggccatcacc agcaacacct
     6241 tcaccatgga gtggccgccg cgctcgggca cccaggcgga gttcccggag gtcgaccgcg
     6301 ccgcgtggtt cacgctcgac gcggcgcggg aggccatcgt gccggtgcag cgccggttcg
     6361 tcgacgacct cgcggtcgtc gcggccgccg gcgcctagga gcgtcccccg gctgcctcgt
     6421 cgtccggggg gtccgccgga tcgggctgcg cgcccaccgg ctgcagtgac ggcaggtgcg
     6481 gcttgccggc gatgaggtcg aagaacgcat cccgccaggc gagcgtgtgc gccctcaggc
     6541 gcgcctcgcg cctctcggcc ctggacagct tcttcggccg cgtccggtag cgccgccgcg
     6601 cccacggcga gttgagccgc gccagccgga tcgcgcccac gatcgcgacg atcggcacga
     6661 agaagccgat caccccggtc cagaccttgc ccttgagcag gcagagcagc accgggacga
     6721 tgaccaccac gatgtaggcg gcgacgacga cgcggaagag gtcgccgctg cgcccgggct
     6781 gctcgaggtc ctcgaacggg tcgatcccga ccacgaacag cgcggcggcg gcgacgacga
     6841 tgatcacggc gtccaccgag agccggccct ccttgcgcca gtacacgtct tggaggttca
     6901 ggatcagggc gaactcgtcc agcgtcagcg cgacaccgac gccgaagagg agcgcgagga
     6961 cctgggcggc gaggccggac ggcgccaggg cgatgagcag cacgcccacg gtgaccatga
     7021 gcaccacgcc gatgaccatg tggtggacgt gggtgccgcc ctcgctctcg atgtcgtgga
     7081 accaccagct cacctgcgcg cggatcagcc gtgtgttgag gcgcacgaag aggaaggtcg
     7141 cgaggaggcc gacgaggaac ccggccatgg ccgcgcggga cccgtccagc cacgccgcgc
     7201 cgacggcgtc ggcggccacg tgcaccatag tgggtcccct ctcgttcgct gcaggtcggc
     7261 gccgccgcgc gcagaacgtg taacggtgcc gggccgggtg cccgtagggc tcctcgggaa
     7321 gagtaacggc agctacggta cccgcttgga ggcgaggtcc caccttgggt cacaacgtcc
     7381 cctcactcgt gaagctggtc ggcgcgcagg gcgagggctg gcgcgcgttc atcggcagcg
     7441 ccgagcgcgg ggagtcgtgg agcgctccgg gggtcagcgt cggtatcgcc ggtgaggccg
     7501 cgcacgaagt caatctggtc gtcgcctacg gcccggacgg cgtgggcgac ggcatcgccg
     7561 cggccgccgc ggtgctccgc cgccgcgggc tgcccggcgc ggtctacgcc gcatccccgg
     7621 tggcggcgga gacggccgag gccgccgccg ccctcggctt caccgcctcc gcccctctgc
     7681 ccctcatgtg cgcgcacgcc ggtgacgtgg tgagcgtcga ggccacgcac gagacggtac
     7741 gcgtcgccgg ggtggagggg gtcctcgccg ccggcgacgt gctggccgac gccttcgacc
     7801 tgcccgtcga ctggtgccag cgcctgctcg gcgtcggttt cgccgcgcgg gccgacgccg
     7861 cggccttcct cgccctccac gacggacgcc ccgtggccgt ggccggctgc gcgcagatcg
     7921 gcgacatcgc cggcctgtat gcggtcggca cgcggcactc acaccggcgg cgcggagcgg
     7981 ccgcggcggc cgtctccgcg gcgatcggcc atcatctg
//
LOCUS       DASUMX010000073         7327 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_163259, whole genome shotgun sequence.
ACCESSION   DASUMX010000073 DASUMX010000000
VERSION     DASUMX010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 7327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7327
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163259"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1645
                     /locus_tag="VFZ86_03355"
     CDS             <1..1645
                     /locus_tag="VFZ86_03355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEX5641358.1"
                     /translation="LDAPKEWPLDGCGTFPPPDQRSLVQEGVQVDRDVEVTLRDGVKI
                     YVDVHRPVGVAGEKDLPAILAWSNYGKRANGGEHAFVPGVPEGAVSNMTKFEGPDPVH
                     WCAQGYAIINTDGRGCGNSEGNQSIWSDQDGRDGYDVVEWAAAQPWSNGKVGMAGNSS
                     LAISQWYVAAEQPPHLACIAPWEGLTDMFREFVTNNGIPAPGFVSFVYTIFRGRGYID
                     DPVAMLSEYPFINGYWKSHMPRLEDVKVPAYVCAGWSHIHLLGTLNAWRRISSEKKWL
                     RIHREFEWPDAYAWQNMEDLRRFFDRYLKGIRNGWEATPPVRLDVMDAYDHDFQVNRP
                     EREYPPARTEYRKLHLDAANAALSPAPVASVSKAAYDAAEGETTFDITFDGDTEITGS
                     MKVRLWVEAEGHDEMDLFITVMKLDENGEWLPTLVMGLPHPGAPGNLRVSRRELDEEL
                     STDVQPVQAHRSEQKLKPGEIVPVDIAIYPTSKIWHRGQQLRLRIAGRYIRENWFEPF
                     AWDTDNKGQHFVHTGGEYDSYLQIPVVPAKYQAGDYVYR"
     gene            1740..2513
                     /locus_tag="VFZ86_03360"
     CDS             1740..2513
                     /locus_tag="VFZ86_03360"
                     /inference="COORDINATES: protein motif:HMM:NF019413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1638 domain-containing protein"
                     /protein_id="HEX5641359.1"
                     /translation="MTLDDQPETLTPGPGRLKFIACPAVLGELADGAADGVDCRELEA
                     QLHVSPERLKEALRAAVAEADVPGATIVLGYGLCSNSVLGLKTEHATLVVPRVDDCIA
                     MLLGSNEAFSAESEKERGSYYVAKAYLEECDTIMSEHEKLVEKRGRERAEMMMRLLLK
                     HYTRIVLVDTGRYDLQPYRARVAEFAETFDLAVEEVPGTTRILDALVAEDWGDDFVVA
                     PPGHELTLQDFRPELFAGGGGGDGADAPSQPTGAGSKPA"
     gene            2510..3097
                     /locus_tag="VFZ86_03365"
     CDS             2510..3097
                     /locus_tag="VFZ86_03365"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX5641360.1"
                     /translation="MNVLCIAGVLGSGKTTVLLEVARAMVERGEQLAIIENEIGEVGV
                     DGGYVREQGLPVQELFGGCICCTLQVGLVETLRTVEGRYEPDWVIIEPTGLAAPGDIL
                     GLVVDNCPHVDTIRVLTLVDAPRWEMLLEVVEPLVTAQLGSADVVAVNKVDEVDADTQ
                     TAVLESVRTLAGTAPVLAVSATSGAGMKSLLGAVL"
     gene            3094..3531
                     /locus_tag="VFZ86_03370"
     CDS             3094..3531
                     /locus_tag="VFZ86_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641361.1"
                     /translation="MTHQHPERGRHAAGGHDHLSHLPSGARPAVFAARAGVVFAQPAH
                     ADQLEDVVTRFFAALSDSLADAGCTLVGHIKGTLAARDRGDLAFHVTALGARPALTGG
                     IAGAPKQGLLTINVIVFGVDEASLPAIVKDAWSRASGAETDWR"
     gene            complement(3659..4999)
                     /locus_tag="VFZ86_03375"
     CDS             complement(3659..4999)
                     /locus_tag="VFZ86_03375"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HEX5641362.1"
                     /translation="MTLPDNWSELTPDERLQWRLDAWQNIPVEFASSEVAQAYRDRVQ
                     LYRDVLDLKKPATVPVAPWIGLLPFRFYGHTGKQAYYDYEIFKGDWFKFHEETRPDGL
                     GLTLGVVPGKLFDILDYNLYTWPGHGTPEDTCYQYTEQEWMKGDEYAALIQDPGTYWQ
                     RTYLPRVFGAMEPFSMLPPWTDLVEIPFTAPFFAGFSAPPVKEMLKKLAEAGDAAMEW
                     LQAAMAADGRCMAELGIPQTAGGATKAPYDTLGDTMRGTRGLMLDKFRQPENVKAAME
                     RLVPQSIDWAVRSNNMTGNPIVFIPIHKGADGFMSDADFKELYWPTFKAVLLGLIEQG
                     LVPWVFVEGSYNERLEVIADEDIPAHRMIWMFDATDMDRAKAVVGKRQCIGGNVPAAL
                     FAMGTPEEMDAYVKDLLGKTAQDGGFILSSGIVLDEADPAAYHAFMQAGRKYGK"
     gene            complement(4996..5631)
                     /locus_tag="VFZ86_03380"
     CDS             complement(4996..5631)
                     /locus_tag="VFZ86_03380"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HEX5641363.1"
                     /translation="MSSGLKEAVIELREEDALRVTEEELKGGTDPLILINACREAMDV
                     IGQRFADGQAFIPELIMAGEIMQGVTARLKPYLKADAAGEKLGTVVMCTVQGDIHDIG
                     KDIVVTMLDIAGFEVIDLGVDVPVAKVVETVRDNNAQVLGLSGLLTVAFDSMRATVAA
                     VDEAGLRADVKIMLGGAPVTDAVVDYAGADAWGKDAVAAVELAKQWAGGAS"
     gene            5812..6594
                     /locus_tag="VFZ86_03385"
     CDS             5812..6594
                     /locus_tag="VFZ86_03385"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX5641364.1"
                     /translation="MVAETGNAKVMDSKPIDRGSFEPPYAQLARAIRERIARGDHRPG
                     DRLPSEAELCAAFKVSPMTVRRAVGELVREDVVVTEHGRGTFVKPPRLAAATFDLDDL
                     TRYLASPDVEAHILEARIVSAGPRVAEKLALEEGGRVISIKRLLLRGEEPVFYHSEYL
                     VFDPARPLVEAELATTALRDLFSGAAGSDLKYGRLTLHASAFTEAEAAQLGEQPGTLA
                     WVLEHVFFDFEDRPESWGRFVGRADRLSFTTTVGITGPKEGA"
     gene            6599..7243
                     /locus_tag="VFZ86_03390"
     CDS             6599..7243
                     /locus_tag="VFZ86_03390"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HEX5641365.1"
                     /translation="MSTPPPKRLDELIVRMDERAALAQIRERLAAGEDPAVLVEECRT
                     GMERVGRLYEEGTYYISALIMAGEIIREATEILIPRMTAVREEIGGGKMVIATVRGDI
                     HDIGKNIVIALLDADGFTMLDLGVNVAPEHIAETVEVERPDVLGLSCLLTSGYEALRE
                     TIELVRKQSEAWHPHLPIVLGGAAVDEMVKERLGADAWCDDAARGLGVVRSLLS"
BASE COUNT         1129 a         2603 c         2487 g         1108 t
ORIGIN      
        1 gctggacgcg ccgaaggagt ggccactgga cggctgcggc acgttcccgc cgccggacca
       61 gcggagcctg gtccaggagg gcgtccaggt cgaccgggac gtcgaggtca cgctgcgcga
      121 cggcgtgaag atctacgtcg acgtccaccg tccggtcggc gtggcggggg aaaaggacct
      181 cccggcgatc ctcgcctggt ccaactacgg caagcgcgcc aacgggggcg agcacgcctt
      241 cgtgcccggc gtacccgagg gcgccgtctc caacatgacc aagttcgagg gccccgatcc
      301 cgtccactgg tgcgcccagg ggtacgcgat catcaacacc gacgggcgcg gctgcgggaa
      361 ctcggagggc aaccagagca tctggagcga ccaggacggc cgcgacggct acgacgtcgt
      421 cgagtgggcc gccgcccagc cgtggtccaa cggcaaggtg ggcatggccg gcaactcctc
      481 gctcgccatc tcccagtggt acgtggctgc ggagcagccc ccgcacctcg cctgcatcgc
      541 gccgtgggaa gggctcaccg acatgttccg tgagttcgtg acgaacaatg gcatccccgc
      601 gccgggcttc gtgagcttcg tctacacgat cttccgcggc cgaggctaca tcgacgaccc
      661 ggtggcgatg ctctccgagt accccttcat caacgggtac tggaaatccc acatgccgcg
      721 ccttgaggac gtcaaggtac cggcctacgt ctgcgccggc tggagccaca tccacctgct
      781 cggcaccttg aacgcctggc ggcgcatctc ctcggagaag aagtggctgc gcatccaccg
      841 cgagttcgag tggccagacg catacgcctg gcagaacatg gaggatctgc ggcgcttctt
      901 cgaccgctac ctcaagggca tccgcaacgg ctgggaggcg accccgcccg tgcgcctcga
      961 cgtgatggat gcctacgacc acgatttcca ggtgaaccgg ccggagcggg agtacccgcc
     1021 ggcgcgcacc gagtaccgca agctccatct ggatgccgcg aacgcggccc tctcccccgc
     1081 gccggtcgcg agcgtctcga aggccgctta cgatgcggcc gagggcgaga ccaccttcga
     1141 catcacattc gacggggaca ccgagatcac cggatccatg aaagtcaggc tctgggtcga
     1201 ggccgagggc cacgacgaga tggacctctt catcaccgtc atgaagctcg acgagaacgg
     1261 cgagtggctc cccacgctgg tcatgggcct gccgcacccc ggcgctcccg gcaacctgcg
     1321 cgtgtctcgc cgggagctcg acgaggagct ctcgacggac gtccagccgg tgcaggcgca
     1381 ccgctctgag cagaagctca agccaggcga gatcgtgccc gtcgacatcg ccatctaccc
     1441 gaccagcaag atctggcaca ggggccagca actgcgactg cgaatcgccg gccgctacat
     1501 ccgcgagaac tggttcgagc cgttcgcctg ggacaccgac aacaaggggc agcacttcgt
     1561 ccacaccggc ggcgagtacg actcgtacct ccagatcccg gtggtgccgg ccaagtacca
     1621 ggcgggggac tacgtctacc gttgagccac gggcgcgcct gcaggcgtcg gcggctcgcc
     1681 gccgacgccc tcctgctacc atgaagtcca ggcccaacgt ccgcgacccc tggtgaccca
     1741 tgaccctcga tgaccagcct gagacactca cgcccggccc cggcagactg aagttcatcg
     1801 cctgcccggc cgtcctcggc gagctcgccg acggcgcggc cgacggcgtc gactgccgcg
     1861 agctcgaggc gcagctgcac gtgagcccgg agcggctcaa ggaggcgctg cgcgccgctg
     1921 tggcggaggc ggacgtcccc ggcgcgacca tcgtcctcgg gtacggtctc tgctccaact
     1981 ccgtcctcgg cctcaagacc gagcacgcca ctctcgtcgt gccgcgcgtg gacgactgca
     2041 tcgccatgct tctcggctcc aatgaggcgt tctcggccga gtcggagaag gagcgcggca
     2101 gctactacgt cgccaaggct tacctcgagg agtgcgacac catcatgagc gagcacgaga
     2161 agctcgtgga gaagcgcggc cgggagcggg cggagatgat gatgcgcctg ctgctcaagc
     2221 actacacgcg catcgtgctg gtcgacaccg gccgctacga cctgcagccg taccgggccc
     2281 gcgtcgccga gttcgccgag accttcgacc tcgcggtcga ggaggtcccc gggacgacgc
     2341 ggatcctcga tgcgctcgtg gccgaagact ggggcgacga cttcgtcgtc gcccctcccg
     2401 gccacgagct caccctccag gacttccggc ccgagctctt cgccggcgga ggcggcggcg
     2461 acggggcgga cgcgccgtcc cagccgaccg gtgccggctc gaagccggcg tgaacgtcct
     2521 ctgcatcgcc ggcgtcctcg gctcgggcaa gacgaccgtg ctgctcgagg tcgcgcgcgc
     2581 gatggtcgag cggggcgagc agctcgccat catcgagaac gagatcggcg aggtcggtgt
     2641 cgacggcggc tacgtccgcg agcagggcct tcccgtgcag gagctgttcg gcggctgcat
     2701 ctgctgcacg ctgcaggtgg gcctcgtcga gacgctgcgc accgtcgagg gccggtacga
     2761 gccggactgg gtgatcatcg agcccaccgg cctggccgcg ccgggcgaca tcctggggct
     2821 tgtggtcgac aactgcccgc acgtcgacac catccgcgtg ctcaccctcg tcgatgcgcc
     2881 gcgctgggag atgctgctgg aggtcgtcga gccgctggtg accgcacagc tcgggtcggc
     2941 cgacgtcgtc gccgtgaaca aggtggacga ggtcgacgcg gacacgcaga ccgcggtcct
     3001 cgagagcgtg cggaccctcg ccggcacggc gccggtgctg gcggtgtcgg ccacctcagg
     3061 cgccggcatg aagagcctgc tcggggcggt gctgtgacgc accagcatcc tgagcgcgga
     3121 cggcatgcgg ccggcgggca cgaccacctc agtcacctgc cgagcggcgc gcggcctgcc
     3181 gtgttcgccg cgcgcgccgg cgtcgtcttc gcgcagccgg cgcacgccga ccagctcgag
     3241 gacgttgtca cccgattctt cgctgcgctc tcggacagtc tggccgatgc cggctgcacg
     3301 ctggtcggcc acatcaaggg caccctggcc gcgcgcgacc gcggcgacct cgccttccac
     3361 gtcacggccc tcggagcccg gccggcgctg accggcggta tcgccggcgc gccgaagcag
     3421 ggcctcctca ccatcaacgt gatcgtcttc ggcgttgacg aggcgtcgct tcccgccatc
     3481 gtcaaggacg cctggtcccg cgcgtccggc gcggagaccg actggcgctg agcccggcct
     3541 gcttcttcgc gccgagggcg gcggcggagg gcactccagt cccccgccgc cgccctctcc
     3601 tcgcgtccga gctcgcctca ggcgcgaccc acctgcgtgg ctccgcgccc gaaggggctc
     3661 acttcccgta cttgcggccc gcctgcatga aggcgtggta cgcggccggg tcggcctcgt
     3721 ccagcacgat gccgctgctc aggatgaagc cgccgtcctg ggcggtcttg cccagcaggt
     3781 ccttcacgta ggcgtccatc tcctccggcg tgcccatggc gaacagcgcc gcgggcacgt
     3841 tgccgccgat gcactggcgc ttgccgacga cggccttggc gcggtccatg tcggtggcgt
     3901 cgaacatcca gatcatgcgg tgtgcgggga tgtcctcgtc cgcgatcacc tcgaggcgct
     3961 cgttatacga gccctcgacg aacacccacg gcaccaaccc ctgctcgatc agcccgagca
     4021 gcacggcctt gaaggtcggc cagtagagct ccttgaagtc ggcgtcgctc atgaacccgt
     4081 cggcgccctt gtggatgggg atgaagacga tggggttgcc ggtcatgttg ttggagcgca
     4141 cagcccagtc gatcgactgc ggcacgagcc gctccatcgc cgccttgacg ttctcgggct
     4201 gacggaactt gtccagcatc agcccgcgtg tgccgcgcat ggtgtcgccc agggtgtcgt
     4261 acggcgcctt ggtggcgccg ccggccgtct gcgggatgcc gagctcggcc atgcagcggc
     4321 cgtcggccgc catcgccgcc tgcagccact ccatggcggc gtcgccggcc tcggccagct
     4381 tcttcagcat ctccttgacg ggcggcgcgg agaacccggc gaagaacggc gccgtgaagg
     4441 ggatctccac gaggtcggtc cacggcggca gcatggagaa cggctccatg gcgccgaaca
     4501 cccgcggcag gtacgtccgc tgccagtacg tgcccgggtc ctggatgagg gcggcgtact
     4561 cgtcaccctt catccactcc tgctcggtgt actggtagca ggtgtcctcg ggcgtgccgt
     4621 gaccgggcca cgtgtagagg ttgtagtcga ggatgtcgaa cagcttgccc ggcacgacgc
     4681 cgagggtcag gcccagcccg tccggcctgg tctcctcgtg gaacttgaac cagtcgccct
     4741 tgaagatctc gtagtcgtag tacgcctgct tgccggtgtg cccgtagaag cggaacggca
     4801 ggaggccgat ccacggcgcg accggcacgg tggccggctt cttgaggtcg agcacgtcgc
     4861 ggtagagttg cacgcggtcg cgataggcct gcgccacctc ggaagaggcg aactcgacgg
     4921 ggatgttctg ccaggcgtcc agacgccact gcagccgttc gtcgggagtg agctcgctcc
     4981 agttgtcggg caacgtcatg acgcacctcc cgcccactgc ttggcgagct ccacggcggc
     5041 caccgcgtcc ttgccccagg cgtcggcgcc ggcgtagtcg accaccgcgt cggtgaccgg
     5101 cgcgccgccg agcatgatct tgacgtcggc gcgcagaccg gcctcgtcga cggcggccac
     5161 ggtggccctc atcgagtcga aggcgacggt gagcagaccg ctgagcccga gcacctgggc
     5221 gttgttgtcg cgaaccgtct ctacgacctt ggcgacgggc acatccacgc ccaggtcgat
     5281 gacctcgaac cccgcgatgt cgagcatggt gaccacgatg tccttgccga tgtcgtggat
     5341 gtcgccctgc acggtgcaca tcaccacggt gccgagcttc tcccccgcgg cgtccgcctt
     5401 gagatacggc ttcagcctgg cggtgacgcc ctgcatgatc tcgcccgcca tgatgagctc
     5461 ggggatgaac gcctggccgt cggcgaagcg ctgaccgatg acgtccatgg cctcacggca
     5521 cgcgttgatg aggatgaggg ggtcagtccc gcccttcagt tcttcctcgg tgacccgcag
     5581 cgcgtcctcc tcacgcagct cgatcacggc ttccttgagc ccggatgaca tcgaacctcc
     5641 ctggtctgtc ctgtcgagcc gcgccaccgc aagaggccgc ggctcctccc tggccggcgc
     5701 cgggcgagcc ggcgtccagc tggtccggcg tcatgatacc agcaactacc ctactggtct
     5761 agggaagtct gctgttgagt ggtagaatgc ggggcatcag gtcgccgggg gatggtggcg
     5821 gaaaccggga acgccaaggt gatggacagc aagccgatcg acagagggtc cttcgaaccg
     5881 ccctacgctc agctcgcgcg agctatccgc gagcgtatcg cgcgcgggga tcaccggcct
     5941 ggcgaccgcc tgccctccga ggccgagctc tgcgcggcgt tcaaggtgag ccccatgacc
     6001 gtgcgccgcg ccgtcggcga actcgtgcgc gaggatgtgg tggtcaccga gcacggccgc
     6061 gggacctttg tgaaaccgcc gcgactggcg gcggccacgt tcgacctcga cgacctcacg
     6121 cgctacctcg cgagccccga tgtcgaggcg cacatcctgg aggcgcgcat cgtctcagcc
     6181 gggccgcgtg tcgccgagaa gctggcgctc gaggagggtg gccgcgtcat ctccatcaaa
     6241 cggctcctcc tgcgcggcga ggagccggtg ttctaccaca gcgagtacct cgtcttcgat
     6301 ccggcgcgcc cgctggtgga ggcggagctg gccacgacgg cgctgcggga cctcttctcc
     6361 ggcgccgccg gctcggacct caagtacggg cgcctgacgc tgcacgcctc cgccttcacc
     6421 gaggcggagg cggctcagct gggcgagcag ccgggaacgc tcgcctgggt cctcgagcac
     6481 gtcttcttcg acttcgagga ccgccccgaa agctgggggc gcttcgtcgg ccgcgccgac
     6541 cgcctgagct tcacgacgac ggtcggcatc accggcccca aggaaggagc atgacgccgt
     6601 gagcacccct ccccccaagc gcctcgacga actcatcgtg cgcatggacg agcgggccgc
     6661 gctcgcgcag atccgcgagc ggctggccgc aggcgaggat ccggcggtcc tcgtggagga
     6721 gtgccgcacg ggcatggaga gagtcggccg cctctacgag gagggcacct actacatctc
     6781 cgcactcatc atggccggcg agatcatccg cgaggcgacc gagatcctca tcccgcggat
     6841 gacggcggtg cgagaagaga tcggcggcgg caagatggtc atcgccacgg tgcgtggcga
     6901 catccacgac atcggcaaga acatcgtgat cgcgctgctc gacgccgacg gcttcaccat
     6961 gctcgacctc ggcgtcaacg tggcgccgga acatatcgcc gagacggtgg aggtcgagcg
     7021 gccggacgtg ctcggcctct cctgcctgct cacgtccggc tacgaggcgc tgcgcgagac
     7081 gatcgagctg gtgcggaagc agtcggaggc ctggcacccg cacctcccca tcgtcctcgg
     7141 cggcgccgcc gtcgacgaga tggtcaagga gcggctcggc gcggacgcct ggtgcgacga
     7201 tgctgcccgc gggctcggcg tcgtgcgctc cctgctctcc tgacgaggcc gcgcggcgtc
     7261 tcgccccccc ggcgagacgc tagccctcac gacgaacggc ggcggggacc tttgcgtgcc
     7321 cccgccg
//
LOCUS       DASUMX010000074         8992 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_452986, whole genome shotgun sequence.
ACCESSION   DASUMX010000074 DASUMX010000000
VERSION     DASUMX010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 8992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8992
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452986"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /locus_tag="VFZ86_03395"
     CDS             <1..723
                     /locus_tag="VFZ86_03395"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HEX5641366.1"
                     /translation="APPEMAGLDIAFTYRSASAGVLSGGDFVDYYTRPPSGSLAFAIG
                     DVAGKGVDAMATTFVTKFMLRGAVHGGQLSWPTRPGEALQELRNGLLEQEDFSPDSER
                     FVTVLFGQLTPRTGLLQLASAGHPTPFLVRAGGVERPLLITEPAIGIELDAALEPYPA
                     ETLRLSRGDLIVLFTDGITELRDEQGAFFESRMAGALSGCHGAGAADVVARLVEAGER
                     FSARPPADDLAVLCIGLTGPLE"
     gene            784..1194
                     /locus_tag="VFZ86_03400"
     CDS             784..1194
                     /locus_tag="VFZ86_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641367.1"
                     /translation="MTRRSRLRSQDGFIREVIWIAVILAVIAVVILDGMAIFRAYQSA
                     DDASSAADEARTEYAQSLNVGAAQQAAEEYLAKSDIEMVDFKAVQTESGTVRFTVTAR
                     ATADTYAFRYLAAIPPLEDWVERTTHPTRTGSAE"
     gene            complement(1201..2268)
                     /locus_tag="VFZ86_03405"
     CDS             complement(1201..2268)
                     /locus_tag="VFZ86_03405"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014794016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HEX5641368.1"
                     /translation="MLRLALIFGGRSAEHEISLASARFVSSMLHHDEYEVTPVGITPA
                     GRWVVPDDFDAATTQGLDAVSSQPVHLVADPTRPGLGLADGSFRPLDFAFPIMHGTFA
                     EDGTIQGLLEMAGLAYAGSGVLGSSVGMDKELMKAVFASAGLPQMDFLSLRDAAATGP
                     EAVAAVEARLGYPVFVKPANLGSSVGMTKAHDRAELVAALDLAAVYDRKTIVEASCDG
                     RELECSLLGNEGVRASAVGEVIPGNEFYDYEAKYTEGKMAFAIPAQVDDRHTERVQEL
                     AVAAFRAVDASGFARCDFFLDTAGQVILNEINTIPGMTAMSGFPRLWAASGLDGKALV
                     GEIVRLGLERHARLARLKMAR"
     gene            complement(2353..2802)
                     /locus_tag="VFZ86_03410"
     CDS             complement(2353..2802)
                     /locus_tag="VFZ86_03410"
                     /inference="COORDINATES: protein motif:HMM:NF025719.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsbRD N-terminal domain-containing protein"
                     /protein_id="HEX5641369.1"
                     /translation="MSSVPFVLRDHREQLWRRWAESLGDDVGADYRELMSSPLGERYV
                     RAFVDDLIAWSEAEEYEAPAQLRQACTRVAADAANRMSLGFSALELATAAQALRGAIV
                     DVLLDALVLGELPSFAETLEQLKAADAFIDRLVVAILGAAPGPAGDV"
     gene            complement(2799..3566)
                     /gene="larB"
                     /locus_tag="VFZ86_03415"
     CDS             complement(2799..3566)
                     /gene="larB"
                     /locus_tag="VFZ86_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HEX5641370.1"
                     /translation="MDRSLDDLFADVAAGRLSAADGAERLRRLGYVSVGDFALLDVSR
                     AARKGVPEVVYAEGKTVEQVVAITRRFLEHDPVVLCSRVSEEQAAALAGVDGAVEHDP
                     RSRIVVVRRADAPAREARGVVGVLAAGTSDVAVAGEAVAMIRAMGVDALTAFDVGVAG
                     LHRLSAPLEAMREAGAGALVVAAGMEGALPSVVAGLAAVPVIGLPTSSGYGYGGRGEA
                     ALMGMLQSCSPGLAVVNIDNGVGAGATAALIARGAAG"
     gene            3694..5220
                     /locus_tag="VFZ86_03420"
     CDS             3694..5220
                     /locus_tag="VFZ86_03420"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX5641371.1"
                     /translation="MPDVPYQLYSSTEQRRRRWPYIVIAAVCVFAAVVGWQLYGVPRV
                     SAVTPGPDAYVKDPSPTVVLDIRGLSDLKDVAVTLDGEDVTAQSRRDGDTLTISAGDL
                     ADGEHAVAFRARSSNLFRRDVSKDWRFTVDTSIPKLELEDELMAGRINTDPAVFSGVT
                     EPYATVTIAGDDVEVSAQADAAGRYSVSADLPDGDSVVVITAADRAGNTRAKRLGVYV
                     DAQPPVLKMTRLDKTLGKSRFTVRIKATDQLGVPAVKFVLDGEERELAGPASKARYAA
                     KDLAQGRHTIVVTVEDKGGNVVTRKETFVVDSTEHFGSATLWPGAKGKDVRKLQSKLA
                     DADVYSGKKTGYFDDATEKAVRSLQAKYGLEVDGIVGGNVLNALSGQIVVDLGDLKLH
                     LYRDGKLVKSYSVATGQSEFPTPTGSYVITSKVMDPTWYPPNSEWAKDAKPIPPGIEN
                     PLGTRWIGTSAPAVGIHGTPDDSSIGTYASHGCIRMHIWDVEDLFDRVVIGLPVVIRR
                     "
     gene            5234..6538
                     /locus_tag="VFZ86_03425"
     CDS             5234..6538
                     /locus_tag="VFZ86_03425"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HEX5641372.1"
                     /translation="MADISPFRSLQYREDIALDAVVAPPYDVLSAQQAAELRARSPYN
                     AVHVDLPVPPGEEPGDEAYARAARTFRGWRQDGVLVRDELPGIVVVDQTYTGPDGRER
                     TRRGFIARLRLATLDQRIVLPHERTHAGPKVDRLRLYRATRADLSQIFLLFADDDRAV
                     GAELETAAAGIPAAAWRTARDDDGNRHLAATVTGSAAERVAEALRDRPLYIADGHHRY
                     ETALAYRDERRAMGDFGADTLMVYLCSLGDPGLTVFPTHRLVRDIEPLTHDALIEHLD
                     PFFQVVGEPVTGAEACRAALEGLPGQADPGRVFGLYLPREETCLTVKTREPAASQRLA
                     AGGCSAAAAGLSVTMLHELLLREALGLEHGDAERHVDYAKSVPDALTRLRSGRYELGA
                     FLNATLVDQVRAIADQGEVMPQKSTYFYPKLLTGLVYDALGD"
     gene            complement(6791..8224)
                     /locus_tag="VFZ86_03430"
     CDS             complement(6791..8224)
                     /locus_tag="VFZ86_03430"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HEX5641373.1"
                     /translation="MQARIQILSEDEKAQVHERTLKVLGAAGMRCDTAEGRRILAAAG
                     ADVDETTRRVRFPADLVESLLSQATRTFTVHGRRPDWSFPVGTGAFTLLADGGATSVF
                     DAAKGERRPTTHDDWRAATRLLDALDDVGFYWCPTDYAPDYEQPGGFVRYVTEVFGTF
                     GKHVQDSFGTPALAPWLKEVLDIVFGGPDQVRARMPLSFLITPASPLTIEHDFTQTWL
                     ELRDYRLPVAVMPMPLQGATAPGSRLGTLLAANCETVGTLCLVQAAAPGTPVFYAPVI
                     ATMDPRTGLYAAGAIEHAVLGVAGTEMARYYGLPAESAGLGTQTYAPDLQTAWEKAES
                     GLLVALSDPDVLVGPGLLGGATVLCLEQIVLDVEIVRHARQARTGVPVRDDLWLDEVL
                     DRVGPCGSFIGERSTRTGVRAGEWRLSDFGVQGSWDAWRTAGAPSTMTAARERVRTVL
                     AGQVSLPYSDDQAAALAALQRRADAAS"
     gene            complement(8292..>8992)
                     /locus_tag="VFZ86_03435"
     CDS             complement(8292..>8992)
                     /locus_tag="VFZ86_03435"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX5641374.1"
                     /translation="AAARAGGRASATECRPPAVILERDLLARLLVVPAATALGLRVLE
                     APAPGGGGPAPAVVFVPLDGEPDCRRVCATARAAFGSPAPPVVAGYGAGPAGLLAAHR
                     AHRCADLVLRLTVSAGEARFAHLPAADAVASAGLTEREADVLVLLLHGCTTPGVAERL
                     CVSPNTARSHCRAVLRKLGAGDRRALRARLLAGASGPPSQPLVTPGFEVCPGSVANFA
                     ERWVVAPPRRAAHT"
BASE COUNT         1319 a         3362 c         3203 g         1108 t
ORIGIN      
        1 gcgccgcccg agatggccgg cctcgacatc gccttcacct accgctccgc cagcgccggc
       61 gtgctcagcg gcggcgactt cgtcgactac tacacgcggc cgccgtccgg cagcctcgcg
      121 ttcgccatcg gcgacgtcgc cggcaagggt gtggacgcca tggccaccac cttcgtcacc
      181 aagttcatgc tgcgcggcgc cgtccacggc ggccagctct cctggcccac gcgccccgga
      241 gaagccctgc aggaactccg caacgggctg ctcgagcagg aggacttctc ccccgacagc
      301 gagcgcttcg tcaccgtgtt gttcgggcag ctcacgccgc gcacgggact gctccagctc
      361 gccagcgccg ggcacccgac gccgttcctc gtgcgcgccg ggggcgtcga gcgcccgctg
      421 ctgatcaccg agccggccat cggcatcgag ctcgacgccg cgctcgagcc gtacccggcc
      481 gagaccctgc ggctctcgcg cggcgacctc atcgtgctgt tcacggacgg gatcaccgag
      541 ctccgcgacg agcagggcgc ctttttcgag tcgcgcatgg ccggggcgct ctccggctgc
      601 cacggcgcgg gcgccgcgga cgtggtcgcc cgcctcgtcg aggcgggcga acggttctcg
      661 gcgcgcccgc ccgcggacga cctcgccgtc ctctgcatcg gcctcacggg gccgttggag
      721 taaactgttc gcgcctcccg ccgatacagg ggggaacgaa gtccggcaga cgaaggagga
      781 acggtgacgc ggcgctcacg cctgcgcagt caggacggct tcatccgcga ggtcatctgg
      841 atcgccgtca tcctcgccgt catcgccgtc gtcatcctcg acgggatggc gatcttccgc
      901 gcctaccagt ccgccgacga cgcgagctcg gccgccgacg aggcgcggac cgagtacgcg
      961 cagtcactca acgtcggggc cgcccagcaa gccgcggagg agtacctcgc caagtcggac
     1021 atcgagatgg tcgacttcaa ggccgtgcag accgagagcg gcacggtcag gttcaccgtc
     1081 acggccaggg cgaccgcgga cacctacgcg ttcaggtacc tggcggcgat cccgccgctc
     1141 gaggactggg tggagcgcac cacgcacccc acgcgcacgg gcagcgccga gtagcggagc
     1201 tcagcgcgcc atcttgagcc gggcgaggcg cgcgtggcgc tcgagtccca gccgcacgat
     1261 ctccccgacc agcgccttgc cgtcgaggcc ggacgccgcc cacagccgcg ggaacccgct
     1321 catggcggtc atgccgggga tcgtgttgat ctcgttgagg atgacctgac ccgcggtgtc
     1381 caggaagaag tcacagcgcg cgaacccgct cgcgtcgacg gcgcggaacg ccgccaccgc
     1441 gagctcctgc acccgctcgg tgtgccggtc gtcgacctgg gcggggatgg cgaaggccat
     1501 cttgccctcg gtgtacttgg cctcgtagtc gtagaactcg ttgcccggga tgacctcgcc
     1561 cacggcggag gcgcgcacgc cttcgttgcc gagcaggctg cactcgagct cgcgcccgtc
     1621 gcaggacgcc tcgacgatcg tcttgcgatc gtagaccgcg gccaggtcga gggccgccac
     1681 gagctccgcc cggtcgtggg ccttggtcat gcccacgctg gagccgaggt tggccggctt
     1741 gacgaacacc gggtagccga ggcgcgcctc gacggccgcg acggcctccg gcccggtcgc
     1801 ggcggcgtcg cgcagcgaga ggaagtccat ctgcggcagg cccgcgctcg cgaacactgc
     1861 cttcatgagc tccttgtcca tgccgacgga gctccccagc acgcccgagc cggcgtaggc
     1921 gaggccggcc atctcgagga ggccctggat ggtgccgtcc tcggcgaacg tgccgtgcat
     1981 gatggggaag gcgaagtcga gcggacggaa cgacccgtcc gccagtccca ggccgggacg
     2041 ggtcgggtct gcgaccaggt ggaccggctg cgaggagacg gcgtccaggc cctgggtcgt
     2101 ggcggcgtcg aagtcgtccg gcacgaccca gcggcccgcc ggcgtgatgc cgaccggggt
     2161 gacctcgtac tcgtcgtggt ggagcatgga ggagacgaag cgagctgagg cgagtgagat
     2221 ctcgtgctcc gcggagcgcc cgccgaagat gagggccaac ctcagcatgc ccggaagcct
     2281 agacgcgcgc cggtgggggc gcaaccctcg cggcgcggcg cgggacggcg caccgcggcg
     2341 tcgctggacc ggtcagacgt cgccggccgg accgggcgcg gcgccgagga tggcgacgac
     2401 gagccggtcg atgaaggcgt ccgccgcctt gagctgctcg agcgtctccg cgaaggaggg
     2461 cagctcgccg agcacgagcg cgtcgagcag cacgtccacg atggcgccgc ggagggcctg
     2521 cgccgccgtc gccagctcga gcgccgaaaa gccgagggac atccggttgg cggcgtcggc
     2581 ggcgacccgc gtgcaggcct gccgcagctg cgccggcgcc tcgtactcct cggcctcgct
     2641 ccaggcgatg agatcgtcga cgaaggcgcg cacgtagcgc tcgccgaggg ggctgctcat
     2701 gagctccctg tagtcggcgc cgacgtcgtc gccgagcgac tcggcccagc gtcgccacag
     2761 ctgctcgcgg tggtcgcgca gcacgaacgg gaccgagctc atccggcggc tccgcgggcg
     2821 atgagcgcgg ccgttgcccc ggcgcccacg ccgttgtcga tgttcacgac cgcgagcccc
     2881 gggctgcagc tctgcagcat ccccatcagc gccgcctcgc cacgcccccc gtagccgtag
     2941 cccgacgacg tcggcaggcc gatcaccggg acggcagcaa ggccggcgac gaccgacggc
     3001 agcgcgccct ccatgccggc ggcgaccacg agggcgccgg cgccggcctc gcgcatggcc
     3061 tcgagcggcg ccgagagccg gtgaaggccg gcgacgccca cgtcgaaggc ggtcagggcg
     3121 tcgaccccca tggcgcggat catcgcgacc gcctcgcccg cgaccgcgac gtcgctggtg
     3181 ccggccgcca ggacgccgac cacgccgcgc gcctcccgcg ccggcgcgtc cgcccggcgc
     3241 acgaccacga tccggctgcg agggtcgtgc tccaccgcgc cgtccacgcc ggccagggcg
     3301 gccgcctgct cctcgctcac ccggctgcag agcaccaccg ggtcgtgctc gaggaaccgg
     3361 cgcgtgatgg cgacgacctg ctcgaccgtc ttgccctccg cgtacacgac ctcgggcacg
     3421 cccttgcgcg cggcccgaga gacgtcgaga agtgcgaagt ctccgaccga gacgtagccc
     3481 aggcggcgca gccgctcggc gccgtccgcg gcggagagcc ggccggcggc gacgtcggcg
     3541 aagaggtcgt cgagagagcg atccatgcgg gagagtatag tgctccctcg cgcgcgactg
     3601 gaagggcggt catacggaaa cacttcagga gagcgaggac agcgccgatg ccctgcgcta
     3661 taatgcggag cagattcgtc caggaagggg cccgtgccgg acgtaccgta tcagctgtac
     3721 agcagcacag agcaacgccg ccgccgctgg ccgtacatcg tcatcgcggc ggtttgtgtg
     3781 ttcgcggccg tggtcggctg gcaactctac ggggtcccgc gggtctccgc ggtgacgccc
     3841 ggtcccgacg cgtacgtcaa ggacccctcg ccgaccgtgg tcctcgacat caggggcctg
     3901 tccgatctca aagacgtggc cgtcaccctc gacggcgagg acgtgaccgc ccagtcccgg
     3961 cgcgacggag acacgctcac catcagcgcc ggcgacctcg cggacggcga gcacgcggtc
     4021 gcgttccgcg ccaggagctc gaacctcttc cgtcgcgacg tgagcaagga ctggcgcttc
     4081 accgtcgaca ccagcatccc caagctcgag ctcgaagacg agctgatggc ggggcgcatc
     4141 aacaccgacc cggccgtgtt cagcggcgtc acggaaccgt acgccaccgt caccatcgcc
     4201 ggcgatgacg tcgaggtgag cgcgcaggcc gacgcggcgg gccggtactc cgtcagcgcc
     4261 gacctgcccg acggagactc ggtggtcgtc atcaccgccg ccgaccgggc cggcaacacg
     4321 cgggccaagc ggctgggcgt gtacgtcgac gcgcagcccc cggtgctcaa gatgacgcga
     4381 ctggacaaga cgcttggcaa gtcccgcttc acggtgcgca tcaaggccac cgaccagctc
     4441 ggcgtgccgg cggtgaagtt cgtgctggac ggcgaagaga gagagctcgc cggccccgcc
     4501 tccaaggcga ggtatgcggc caaggacctc gcccagggca ggcacaccat cgtcgtcacg
     4561 gtcgaggaca agggcggcaa cgtggtcacc cgcaaggaga ccttcgtcgt cgacagcacc
     4621 gagcacttcg gctccgccac cctctggccg ggcgccaaag gcaaggacgt caggaagctc
     4681 cagtccaagc tcgccgacgc ggacgtctac tccggcaaga agaccgggta cttcgacgac
     4741 gccaccgaga aggccgtgcg cagcctgcag gccaagtacg gcctggaggt cgacggcatc
     4801 gtcggaggca acgtgctcaa cgccctcagc ggccagatcg tggtcgacct gggcgacctc
     4861 aagctgcacc tgtaccgcga cggcaagctc gtcaagagct actccgtcgc gaccgggcag
     4921 tcggagttcc ccacgcccac gggctcctac gtgatcacct ccaaggtgat ggaccccacc
     4981 tggtacccgc ccaactcgga gtgggcgaag gacgccaagc ccatcccgcc gggcatcgag
     5041 aatccgctgg gcacgcgctg gatcggcacc agcgcccccg ccgtcggcat ccacggcacg
     5101 ccggacgact cctccatcgg cacctacgca tcccacggct gcatccgcat gcacatctgg
     5161 gacgtcgaag acctcttcga tcgcgtggtc atcggcctgc cggtcgtcat ccgccggtag
     5221 cggccgcccc gccgtggccg acatctcgcc cttcaggagc ctccagtacc gcgaggacat
     5281 cgcgctcgac gccgtggtcg cgcctcctta cgacgtgctc tcggcgcagc aggccgcgga
     5341 gctccgcgcg cgcagcccgt acaacgcggt ccacgtggac ctgccggtgc cgcccggaga
     5401 agaacccggc gacgaggcct acgcccgcgc cgcgcgcacc ttccgcggct ggcggcagga
     5461 cggggtgctg gtacgcgacg agctgccggg catcgtcgtc gtcgaccaga cgtacaccgg
     5521 gccggacggc cgcgagcgca cccgcagggg cttcatcgcg cggctccggc tcgccaccct
     5581 ggaccagcgc atcgtgctgc cgcacgagcg cacccacgcg ggccccaagg tcgaccgcct
     5641 gcggctgtac cgggccaccc gcgcggacct cagccagatc ttcctgctct tcgcggacga
     5701 cgaccgcgcc gtcggcgcgg agctcgagac ggcggcggcc ggcatccccg ccgccgcgtg
     5761 gcgcaccgcc cgtgacgacg acggcaaccg gcacctcgcg gcgacggtga ccggatcggc
     5821 cgcggagcgc gtcgccgagg cccttcgcga ccgcccgctc tacatcgccg acgggcacca
     5881 ccgttacgag acggcgctgg cctaccgcga cgaacgccgg gcgatgggcg acttcggcgc
     5941 cgacaccctc atggtctacc tgtgcagcct cggcgatccc gggctcaccg tgttccccac
     6001 gcaccggctg gtgcgcgaca tcgagccgct cacgcacgac gcgctcatcg agcatctcga
     6061 cccgttcttc caggtcgtgg gcgagccggt gaccggggcc gaggcctgcc gcgcggcgct
     6121 cgagggactg cccgggcagg ccgaccccgg cagggtcttc ggcctgtatc tgccccgcga
     6181 ggagacgtgc ctcacggtca agacccgcga gccggccgcg tcgcagcggc tcgcggccgg
     6241 cgggtgctcc gccgccgccg ccggcctctc tgtgaccatg ctccacgaac tgctgttgcg
     6301 ggaggcgctc ggcctggagc acggcgacgc cgagcgccac gtcgactacg ccaagagcgt
     6361 gcccgacgcg ctcacccgcc tgcggtcggg acggtacgag ctcggggcgt tcctcaacgc
     6421 gaccctggtc gaccaggtgc gggccatcgc cgaccagggc gaggtcatgc cgcagaagtc
     6481 cacgtacttc taccccaagc tgctgaccgg cctggtctac gacgcgctcg gggattgagc
     6541 gtcgaaggcc gttcaacgag tggggcgccg aaggtgcgcc gctcaccggc gccgggcggc
     6601 gggtgacgcc ggcgggtgac gccggcccag cggccgcgag gtgatcaaca ggcagccttc
     6661 gtgccggcga ctgctccctg atctcctcga agagatcatc ctcaaaggca gccggcgggg
     6721 cgtgacggcg tcccgggccc gggcggctac gccggcggtc acgccggacg cgtgcgcgga
     6781 ggccgcgaag tcaggaggcg gcgtcggcgc ggcgctgcag cgccgcgagg gccgccgcct
     6841 ggtcgtcgct gtagggcagg gagacctggc cggcgaggac ggtccggacg cgctcgcgcg
     6901 cggcggtcat ggtgctcgga gcgcccgccg tccgccacgc gtcccagctg ccctgcacgc
     6961 cgaagtcgct gagtcgccac tcgccggcgc gcaccccggt gcgcgtcgac cgctcgccga
     7021 tgaacgaacc acacggcccg acgcggtcca ggacctcgtc cagccagagg tcgtcacgca
     7081 ccggcacgcc ggtgcgcgcc tggcgggcgt gccggacgat ctccacgtcc aggacgatct
     7141 gttcgaggca gaggaccgtc gctccgccga gcaggcccgg gccgaccagc acgtccgggt
     7201 cggacagcgc caccagcagg ccgctctccg ccttctccca cgcggtctgc aggtcgggcg
     7261 cgtaggtctg cgtgccgagc ccggcggact cggccggcag gccgtagtac cgggccatct
     7321 cggtgccggc gacgcccagc accgcgtgct cgatcgcgcc ggcggcgtaa aggcccgtgc
     7381 gcgggtccat ggtcgcgatc acgggggcgt agaacaccgg cgtgcccggc gctgccgcct
     7441 gcacgaggca cagcgtgccc acggtctcgc agttggcggc cagcagcgtg cccaggcggc
     7501 ttcctggcgc ggtggcgccc tgcagcggca tcggcatcac tgcgaccggc aagcggtagt
     7561 cgcgcagctc gagccaggtc tgcgtgaagt cgtgctcgat ggtcagcggc gacgccggcg
     7621 tgatgaggaa cgacagcggc atgcgcgcgc gcacctggtc gggtccgccg aacacgatgt
     7681 ccaggacctc cttgagccac ggcgcgagcg cgggggtgcc gaacgaatcc tggacgtgct
     7741 tgccgaaggt gccgaacacc tcggtgacgt agcgcacgaa cccgccgggc tgctcgtagt
     7801 ccggcgcgta gtcggtcggg caccagtaga agccgacgtc gtccagggcg tcgagcagcc
     7861 gggtcgccgc ccgccagtcg tcgtgggtcg tcgggcggcg ctctcccttc gcggcgtcga
     7921 acaccgaggt ggcgccgccg tccgccagca gggtgaaggc gccggtcccg accgggaagc
     7981 tccagtcagg ccggcgcccg tgcacggtga acgtcctcgt cgcctgcgag agcagagact
     8041 cgacgaggtc ggccgggaac cgcacccggc gcgtcgtctc gtcgacgtcc gccccggccg
     8101 cggccaggat gcgacgcccc tcggcggtgt cgcagcgcat gcccgcggcg cccagcacct
     8161 tcagcgtgcg ctcgtggacc tgcgccttct cgtcctcgga gaggatctgg atccgtgcct
     8221 gcatcagcca ccccccgtcg acatccggcc ccgcgcctcg tgcccaccat agtcggcgcg
     8281 gcgacgcgcc ctcaagtatg ggccgcgcgc cgaggcggcg cgacgaccca tctctcggca
     8341 aagttcgcga cgctgcccgg acaaacctcg aagccgggcg tcacgagcgg ctgagacgga
     8401 gggccggagg cgccggcgag gaggcgggcg cgcagcgccc tccggtcgcc ggcccccagc
     8461 ttgcgcagca cggcgcggca gtggctgcgc gccgtgttcg gggagacgca gagccgctcc
     8521 gccacgcccg gcgtggtgca cccatggagg agcagcacga ggacgtcggc ctcgcgctcg
     8581 gtgaggccgg cggaggcgac ggcgtccgcc gccggcagat gcgcgaaccg cgcttctccg
     8641 gcgctgacgg tgagccgcag gacgaggtcc gcgcagcggt gggcgcgatg cgcggccagc
     8701 aggccggcgg gaccggcgcc gtagccggcc acgacgggcg gcgccggcga gccgaaggcc
     8761 gcccgggccg tggcgcagac gcgacggcag tccggctccc cgtcgagcgg gacgaacacg
     8821 accgccggcg ccgggccgcc gccgccgggc gccggcgcct cgagcacccg caggcccagc
     8881 gccgtcgccg ctggcacgac caggaggcgc gccagcaggt cccgctcgag gatcacggcc
     8941 gggggacggc actccgtcgc cgacgctcgg cccccggcgc gtgccgccgc gg
//
LOCUS       DASUMX010000075        11978 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_597327, whole genome shotgun sequence.
ACCESSION   DASUMX010000075 DASUMX010000000
VERSION     DASUMX010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 11978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11978
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_597327"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="VFZ86_03440"
     CDS             <1..257
                     /locus_tag="VFZ86_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008681101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEX5641375.1"
                     /translation="TMQTLIEAGCAFSTPTCGACLGGHMGVLAEGERAVATTNRNFVG
                     RMGHPGAEVYLASPYVAAASALAGHIATPEEVGAVKGGSR"
     gene            254..757
                     /locus_tag="VFZ86_03445"
     CDS             254..757
                     /locus_tag="VFZ86_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009306194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEX5641376.1"
                     /translation="MIFEGTVHKYGRDVDTDVIIPARHLNTNDPQELASHAMEDIDAG
                     FVDRVQQGDIIVADENFGCGSSREHAPVAIKHSGVSVVVAASFARIFYRNAINTGLPI
                     VVCPAAAADAEMGDRLKVDLAAGTVENLTQGKTYTSEAFPPFMQELIDRGGLLEYVKS
                     RVGEHGA"
     gene            803..1885
                     /locus_tag="VFZ86_03450"
     CDS             803..1885
                     /locus_tag="VFZ86_03450"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEX5641377.1"
                     /translation="MGKTYKIAVLPGDGTGPEVVAEGLKVLDAAAAKHGFKLDLTHYD
                     LGGERHLRTGEILPESVIEELRGVDAIYLGAIGHPDVAPGILEKGLLLQLRFELDQYI
                     NLRPVRLYPGVETPLKDKGPADIDYVVVRENSGDVYAGGGGTMMPGSPHEVATQLMVY
                     SRFQVDRCLRWAFEYARQHGKKARGLGEHNTLGLVGKTNVLTFVYDLWFRAFNEMGEA
                     EYPDVEREYYHVDATCMWMVKSPEWFDVLVTGNMFGDIITDLGAITQGGMGVAAGGNI
                     NPEGVSMFEPIGGSAPKYTGMGVINPIAAIAAGAMMLETLGEVRAAEDVERAAKEVTG
                     TRMKSMASGQMGYSTSEVGDLVAERV"
     gene            1961..2884
                     /gene="ilvE"
                     /locus_tag="VFZ86_03455"
     CDS             1961..2884
                     /gene="ilvE"
                     /locus_tag="VFZ86_03455"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009305975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HEX5641378.1"
                     /translation="MAPIPEMDTIWMDGALVPWADAKVHVLTHALHYGSGVFEGIRAY
                     KTERGVAVLRLTEHLKRIVRSAKLYYMPVPYSTDELYDATFEVLASNKLEACYIRPLI
                     FRGYGEMGLNPMDAPVQVIIAAWPWGTYLGDEGIKHGVRAKTSSIQSLDHTALARAAK
                     ATGQYLNSILAKVEVLNAGYDEAVMLTEHGHVAEGSGENIFCVRNGVLMTPPPSDGVL
                     EGITRDIVVKLAASEGIPFQERTLTRSDLVIADEAFYTGTAAEVVPIRELDDHLIGEP
                     GPVTRRMQERFAAITSGKDEEFAGFMEYARK"
     gene            2907..4517
                     /gene="cimA"
                     /locus_tag="VFZ86_03460"
     CDS             2907..4517
                     /gene="cimA"
                     /locus_tag="VFZ86_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013980679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HEX5641379.1"
                     /translation="MHERRVQLYDTTLRDGMQQEGMSVSVEEKVRIALKLDELGIGFI
                     EGGFPASNPKEIEFFRRMQGERLESAELVAFGMTRRKGAAAESDEGLRVLAEIFTPTV
                     TIVGKTWGLHLSKVIRVSRGENLQMIDESVRFLAAQGKRVVYDAEHFFDAWRDDREYA
                     LRCVATAAGAGATAVCLCDTNGASLPPFVADVVREVTAALPEVVVGVHIHNDSDCAVA
                     GSLIAVEAGAGQVQGTVNGYGERCGNANLVSIIPALKLKMGRDVVSDAQLARLTETSH
                     FVADVVNAAIWAHAPYVGRSAFAHKGGMHVAAVEKAPQTFEHVDPEKVGNEQRIVVSE
                     LSGKGAVVRKAQELGLRLEGDDARVATVLKRLKAREHEGYHYEAADASFDLLLRQELA
                     PHEPMFRLESFRVIVEKREDGRAVVEATIKIHVGGERIISTAEGNGPVNALDSALRQA
                     IVGKYPHLADIELVNYKVRILDETKGTGAVTRVLLDASDGEESWGSIGVSENVVEASW
                     EALVDSIEFGMLRGAGTTAANDAQGEAR"
     gene            4514..4693
                     /locus_tag="VFZ86_03465"
     CDS             4514..4693
                     /locus_tag="VFZ86_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011357736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEX5641380.1"
                     /translation="MSDADKVLEIMAEAGEPLNAGAIAERSGMDRKAVDKAMAELKKD
                     GRIESPKRCYWAPKA"
     gene            complement(4672..5127)
                     /locus_tag="VFZ86_03470"
     CDS             complement(4672..5127)
                     /locus_tag="VFZ86_03470"
                     /inference="COORDINATES: protein motif:HMM:NF023580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HEX5641381.1"
                     /translation="MRELWRRLGRLNRVSLVIVAVVFVVCVGLVVVVDPIIGSILAAT
                     CTGLTAFSLWFFFHDEVRNDRLRDGGVRAEAVILSMEETGVTIQGNYLQARLRLLVRP
                     ETGESYEATTKCLMNRLEVPAYQPGNHIPVVVDPKDPTRVGVDQALGAQ"
     gene            5283..8696
                     /locus_tag="VFZ86_03475"
     CDS             5283..8696
                     /locus_tag="VFZ86_03475"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEX5641382.1"
                     /translation="MTSAFVHLHVHSEYSLLDGACRIKELVKRAKELGMPAVALTDHG
                     TLGGAVKFYRAAEEEGIKPIIGLELYVATDRRSRAGVKERNAHLTLLARDETGYKNLV
                     RLATTAYLEGYYYKPRADWELLSQHHEGLIALTGCMSGKTAMLLRDGDDSAALAEVRR
                     LADLLGPENVYVELQDAGLPEQRELLPKLAHLAEKAGLKTVATNDVHYLRHEDAYAHD
                     ALLCIQTQSCLTDEKRMKYGSDEFYLKSAEEMEERFSDYPGATAATVEIAERCNVGLD
                     FGDYKLPTYPVPEGFTEDSYLRDLCEQGIARRYGAEPGQEVIERLDFELGVIGEMGFD
                     AYFLIVWDYVKFAKDNGIAVGPGRGSAAGSIVSYALGITDIDPLKYDLLFERFLNPGR
                     KSMPDIDMDFSVDRRGEVIDYVARKYGRDRVAQIITFGTMAARAAVRDAARVMGKPYA
                     VGDKIAKMIPEQAPPASFSDAMLPGCELKQAYDADEEVKEVVDLAMSLEGLIRNDSIH
                     AAAVVISDRPLTDYLPLQQKGEAELVTQFDMNDVSKLGLLKMDFLGLRNLDVIEAALV
                     IIEETRGVCIEIDRLPLDDEKTYKMLARGDSTGVFQFESPGMKEALRDIGPTEFEDLI
                     AIVALYRPGPMQYIPTYARNKKDPASVVYDHESLRPILEPTHGVTIYQEQYMAIARRV
                     GGFSPAQADDLRKAISKKNHTLMATLKEPLMKGLAEKDVPPAVANKMWANFEATGDYS
                     FNKSHAACYAMISYRTAWLKANYPVEYMAALVSSVMNTKDKVPFYVNQCHEMGIEVLP
                     PDVNTSQVGFTVVEGKIRFGMNAVKGVGVGAIEAIIRAREEGGPFASVFDFCSRVDSQ
                     MANKRVLEALIRAGAFDSTGDPRRGMIEVLPLAMSDGERKRKDAANGQGDLFGAMDSG
                     DTGHPLPTVPRSEWDQTTLLRYEKEALGLYVSAHPLQGLREQLRDEIEMPVSALDNAG
                     DGAMTWTGGIVANAQKRVSKNGGVWLSFRLEDVDGGIECRAWPKVYEQFRELLVEDAI
                     IKVKGRIEKRAEGDSMLMAIEVVPFNGVSEYHPLTVVIDSACVAPTVMDDLRRVLTDF
                     PGTVPVVLQMVSGEQRARLRVGDGLRVEPVAGLYAELKALLGESCIGVG"
     gene            8724..9923
                     /gene="hisZ"
                     /locus_tag="VFZ86_03480"
     CDS             8724..9923
                     /gene="hisZ"
                     /locus_tag="VFZ86_03480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEX5641383.1"
                     /translation="MIPEGMRDVLPPETGRLRAVEDRLRDRFAAYGYGEVRTPWLEFA
                     ETLESSGDDTLSAGYRLHDEQGHQLMVRTDMTVPVARMAADRCDDDPLPLRFSYVAPC
                     IRPWAPQRGQDGEFQQAGAELLGLDSAEADAECVTLLCDALAGLGLRAFTVTLGSAAY
                     QRALIDSLGLPDEDRETFTEALADRDYPLVESVAGNADVEYDALKALWRVLELSGGEE
                     TLGRARKLARNEVMEAAVARLARVRDLVEEAGHGDRLAFDFGLMQDLSYYSGLIVEAY
                     APGVGLPVATGGRYDGLLARFEWDIPGVGFAVAVDRAADALDEAGVELVSAPAAVPFV
                     GGLEAPARAAELRRAGIAVLALPEDVRAVAPPLLLRHGGSYTLRLADGREVTGGPGEI
                     AEALGSG"
     gene            9920..10543
                     /locus_tag="VFZ86_03485"
     CDS             9920..10543
                     /locus_tag="VFZ86_03485"
                     /inference="COORDINATES: protein motif:HMM:NF013773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641384.1"
                     /translation="MRIGLACEAGAPPADVLGLLEAAGLPAASLRGEAAPALLPHPRG
                     VWFLGSAADVLRACDRGALDAGVVGSDELLEGRHGVADLLDLRCRRDDLVFAVAGPGG
                     RPDRRLRVATRHPEAARRYFAAAGLQPELIELDAPALAPALGLAAGVVELRARLQAGA
                     VEPPVLVEQGVVAACSARLVAGRSARVLRRDDVNALADALRAVVEER"
     gene            10540..11811
                     /gene="hisD"
                     /locus_tag="VFZ86_03490"
     CDS             10540..11811
                     /gene="hisD"
                     /locus_tag="VFZ86_03490"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEX5641385.1"
                     /translation="MKHLPWNDDVAATVAQLRALAPPPAEVRKAAEAIVADVRARGDA
                     AVREQTARLDRVELAEHYAVPAAEMRARLEALKPELRAALETAAANIRAYHEREAVRP
                     WRETLAQGQVVGQEVVPLGAAGLYAPGGLADYPSSLLMTAIPAQVAGVERIVVCSPPR
                     PGGGAAAGVAAACALIGIEHLYPIGGAQAVAAMALGTACVPRCDVIAGPGNAYVTEAK
                     RLLVGEVGIDSLAGPSEVLIIADSSGRPEWLAADLLAQAEHGMGAMACLVDVGGALAG
                     PVAAAVDRLSAELGIATDNVVLVACPDRGAALTLSNAFAPEHLELHCEGARELLAGVR
                     NAGAVFVGPHAATAFADYAAGSNHVLPTGGSARFGQGLSVDAFRRRMAVVELDGPAAA
                     ALAAQVATIADEEGLRAHALSARLRASEELT"
     gene            11808..>11978
                     /locus_tag="VFZ86_03495"
     CDS             11808..>11978
                     /locus_tag="VFZ86_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009014777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase"
                     /protein_id="HEX5641386.1"
                     /translation="MIERRAEVVRTTTETDISLTLDLDGTGAADVVTGIGFLDHMLTL
                     LAHHGGFDLTVRA"
BASE COUNT         1900 a         4303 c         4186 g         1589 t
ORIGIN      
        1 gcaccatgca gacgctcatc gaggccggct gcgccttcag cacccccacc tgcggcgcct
       61 gcctgggcgg ccacatgggc gtcctcgccg agggtgagcg cgccgtggcc accaccaacc
      121 gcaacttcgt cggccgcatg ggtcaccccg gcgccgaggt ctacctggcc agcccgtacg
      181 tggccgcggc ctccgcgctc gccggccaca tcgccacgcc cgaagaggtc ggcgccgtga
      241 agggaggctc ccgatgatct tcgagggcac cgtccacaag tacggccggg acgtcgacac
      301 cgacgtcatc atcccggcgc gccacctcaa caccaacgac ccgcaggagc tcgcgagcca
      361 cgcgatggag gacatcgacg ccgggttcgt ggaccgcgtc cagcagggcg acatcatcgt
      421 cgccgacgag aacttcggct gcggcagctc gcgcgaacac gctcccgtgg cgatcaagca
      481 ctcgggcgtg agcgtcgtcg tcgcggcgtc gttcgcgcgc atcttctacc gcaacgccat
      541 caacaccggc ctgccgatcg tcgtctgccc ggccgcggcc gccgacgccg agatgggcga
      601 ccgcctcaag gtcgacctgg ccgccggcac cgtcgagaac ctcactcagg gcaagaccta
      661 tacgtcggag gcgttcccgc cgttcatgca ggagctcatc gaccgcggcg gcctgctcga
      721 gtacgtcaag tcgcgcgtgg gcgagcacgg cgcctgacca ccggcacaca gcacaagcat
      781 ccagcatcgg agagaaagac acatggggaa gacctacaag atcgcagtac tgcccggcga
      841 cggcacggga cccgaggtcg tcgccgaggg tctcaaggtc ctcgacgcgg ccgccgcgaa
      901 gcacggcttc aagctcgacc tcacgcacta cgacctcggc ggtgagcgcc atctgcgtac
      961 cggcgagatc ctgcctgagt cggtgatcga ggagctcagg ggggtcgacg cgatctacct
     1021 gggcgccatc gggcacccgg acgtggcgcc gggcatcctg gagaagggcc tgctcctcca
     1081 gctgcgcttc gagctggacc agtacatcaa cctgcgtccc gtgcgcctgt atcccggcgt
     1141 cgagacgccg ctcaaggaca agggccccgc ggacatcgac tacgtcgtcg tgcgcgagaa
     1201 ctccggagac gtgtacgccg gcggcggcgg cacgatgatg ccgggctcgc cgcacgaggt
     1261 cgccacccag ctcatggtgt acagccgctt ccaggtggac cgctgcctgc gctgggcgtt
     1321 cgagtacgca cgccagcacg gcaagaaggc gcgcggcctc ggcgagcaca acacgctcgg
     1381 cctggtgggc aagaccaacg tgctcacctt cgtgtacgac ctctggttcc gcgccttcaa
     1441 cgagatgggc gaagccgagt acccggacgt cgagcgcgag tactaccacg tggacgccac
     1501 ctgcatgtgg atggtcaaga gccccgagtg gttcgacgtg ctcgtcaccg gcaacatgtt
     1561 cggcgacatc atcaccgacc ttggcgcgat cacgcagggc ggcatgggcg tcgcggcagg
     1621 cggcaacatc aaccccgagg gcgtcagcat gttcgagccc atcggcgggt ccgcccccaa
     1681 gtacaccggt atgggcgtga tcaatccgat cgccgccatc gccgccggcg cgatgatgct
     1741 cgagacgctg ggcgaggtcc gggcggcgga ggacgtggag cgtgccgcga aagaggtcac
     1801 cggcaccagg atgaagagca tggcctccgg gcagatgggc tactcgacca gcgaggtcgg
     1861 cgacctcgtg gccgagcgcg tctgactggg aggctccact aacgctcagg taaaggttga
     1921 agcttcgcgc ggggaccgcg cgaaggacag gaggaagacg atggccccga tccccgagat
     1981 ggacacgatc tggatggacg gcgcgctggt cccctgggcc gacgccaagg tccacgtgct
     2041 cacgcacgcg ctgcactacg gctccggggt cttcgagggc atccgcgcgt acaagacgga
     2101 gcgcggcgtc gccgtgctgc gcctcaccga gcatctcaag cgcatcgtgc gctccgccaa
     2161 gctctactac atgccggtcc cgtacagtac cgacgagctg tacgacgcga ccttcgaggt
     2221 gctcgcgtcc aacaagctcg aggcctgcta catccggccg ctgatctttc gcggttacgg
     2281 cgagatgggc ctgaacccga tggacgcgcc ggtgcaggtc atcatcgcgg cgtggccgtg
     2341 gggcacctac ctcggcgacg agggcatcaa gcacggcgtg cgcgccaaga cgagcagcat
     2401 ccagtcgctc gaccacacgg cgctggcccg cgccgccaag gccacgggcc agtacctcaa
     2461 cagcatcctc gccaaggtcg aggtcctcaa cgccggctac gacgaggccg tgatgctcac
     2521 ggagcacggc cacgtcgccg agggctcggg cgagaacatc ttctgcgtgc gcaacggcgt
     2581 cctgatgacg ccgccaccga gcgacggcgt gctcgagggc atcacgcgcg acatcgtcgt
     2641 caagctcgcc gcaagcgagg gcatcccgtt ccaggagcgc acgctcacgc gcagcgacct
     2701 ggtgatcgcc gacgaggcgt tctacacggg cacggccgcc gaagtggtgc ccatccgcga
     2761 gctcgacgac cacctcatcg gcgagcccgg ccccgtcacg cgccgcatgc aggagcgctt
     2821 cgcggcgatc acctcgggca aggacgagga gttcgccggc ttcatggagt acgcgaggaa
     2881 gtagcgggag ggaccgggga ggaccgatgc acgagcgccg ggtacagctc tacgacacca
     2941 cgctccgcga cggcatgcag caggagggca tgtcggtctc ggtcgaggag aaggtgcgca
     3001 tcgcgctcaa gctcgacgag ctcggcatcg gcttcatcga gggcgggttc ccggcctcga
     3061 accccaagga gatcgagttc ttccggcgca tgcaaggcga acgcctggag agcgcggagc
     3121 tcgtcgcctt cggcatgacg cggcgcaagg gcgcggcggc agagtccgac gagggccttc
     3181 gcgtgctcgc cgagatcttc acgcccaccg tcaccatcgt cggcaagacc tggggcctgc
     3241 atctgagcaa ggtcatccgc gtgagtcggg gcgagaacct gcagatgatc gacgagtcgg
     3301 tgcgctttct cgcggcgcag ggcaagcgtg tggtgtacga cgccgagcac ttcttcgacg
     3361 cctggcgcga cgaccgcgag tacgcgctgc gctgcgtcgc cacggccgcc ggggcgggcg
     3421 ccacggccgt ctgtctgtgc gacaccaacg gggccagcct gccgccgttc gtcgcggacg
     3481 tcgtccgcga ggtcacggcg gctctgcccg aggtcgtcgt gggcgtgcac atccacaacg
     3541 acagcgactg cgccgtcgcc ggcagcctga tcgccgtcga ggccggcgcc ggtcaggtgc
     3601 agggcaccgt gaacggctac ggggagcgct gcggcaacgc caacctcgtc agcatcatcc
     3661 cggcgctcaa gctgaagatg ggccgcgacg tggtgagcga cgcgcagctc gcgcgcctca
     3721 ccgagacgtc gcacttcgtc gccgacgtgg tcaacgcggc catctgggcg cacgcgccct
     3781 acgtgggccg ctccgccttc gcccacaagg gcggcatgca cgtggccgcc gtggagaagg
     3841 cgccgcagac gttcgagcac gtcgaccccg agaaggtggg caacgagcag cggatcgtgg
     3901 tctcggagct ctcgggcaag ggcgccgtgg tgcgcaaggc gcaggagctc ggcctccggc
     3961 tggagggcga cgacgcgcgc gtggccaccg tgctcaagcg gctcaaggcg cgcgagcacg
     4021 agggctacca ctacgaggcg gccgacgcct cgttcgacct gctgctgcgc caggagctgg
     4081 cgccgcacga gccgatgttc cggctcgaga gcttccgcgt catcgtcgag aagcgcgagg
     4141 acggccgcgc cgtggtcgag gcgacgatca agatccacgt gggcggcgag cgcatcatca
     4201 gcacggccga gggcaacggc ccggtcaacg cgctggactc ggcgctgcgc caggcgatcg
     4261 tgggcaagta cccgcacctc gccgacatcg agctggtcaa ctacaaggtg cgcatcctcg
     4321 acgagaccaa gggcaccggc gccgtcaccc gcgtgctgct ggacgccagc gacggggagg
     4381 agagctgggg cagcatcggc gtgagcgaga acgtcgtcga ggcgagctgg gaggcgctgg
     4441 tcgactccat cgagttcggg atgctgcgcg gcgcgggtac aaccgccgca aacgacgcac
     4501 aaggagaggc gcgatgagcg atgccgacaa ggtcctcgag atcatggccg aggcgggcga
     4561 gccgctcaac gccggcgcga tcgccgagcg ctccggcatg gaccgcaagg ccgtggacaa
     4621 ggcgatggcc gagctgaaga aggacggccg tatcgagtcg cccaagcgct gctactgggc
     4681 gcccaaggcc tgatcgacgc cgaccctcgt cgggtccttc gggtcgacca cgaccgggat
     4741 gtggttgccg ggttggtagg cagggacctc gaggcggttc atcaggcact tcgtcgtcgc
     4801 ctcgtacgat tcgccggtct ccggccgtac cagtagccgc agccgggcct gaaggtagtt
     4861 gccctggatg gtcacccccg tctcctccat ggagaggatg accgcctcgg cgcgcactcc
     4921 cccgtcgcgc agccggtcgt tgcgcacctc gtcgtggaag aagaaccaga ggctgaaggc
     4981 cgtcagtccg gtgcaggtcg cggcgaggat cgacccgatg atcgggtcga ccacgacgac
     5041 aagccccacg cagaccacga acaccacggc gacgatgacc aggctcaccc ggttgaggcg
     5101 gcccaggcgc cgccagagct cccgcatcgc caccctcctg tcgagagacc gcgactcgtc
     5161 tcgagactac gccctcgtca tcctacgcga gcggcgcgac ggcggtaccc ccggggggtt
     5221 tcccgacggg ccgccgaacg cctccggggc ggcccccctc catgctagaa tccggcccat
     5281 ccatgacctc cgcattcgtc catctccacg tgcacagcga gtactcgctg ctcgacggcg
     5341 cctgccggat caaagagctg gtcaagcggg ccaaggagct gggcatgccg gcggtggcgc
     5401 tcaccgacca cggcaccctc ggcggcgccg tcaagttcta ccgcgccgcc gaagaagagg
     5461 gcatcaagcc catcatcggt ctcgagctgt acgtggccac cgaccgccgc agccgcgccg
     5521 gcgtcaagga gcgcaacgcg cacctcacgc tgctggcgcg cgacgagacc ggctacaaga
     5581 acctcgtcag actcgccacc acggcctacc tcgagggcta ctactacaag ccgcgtgccg
     5641 actgggagct gctcagccag caccacgagg ggctcatcgc gctcaccggc tgcatgagcg
     5701 gcaagaccgc catgctgctg cgcgacggcg acgactcggc cgcgctggcc gaggtccgcc
     5761 gtctggcgga cctgctcggc ccggagaacg tctacgtgga gctgcaggac gccggcctgc
     5821 ccgagcagcg cgagctgctg cccaagctcg cgcatctcgc cgagaaggcc ggcctcaaga
     5881 cggtggccac caacgacgtc cactacctgc gtcacgagga cgcctacgcc cacgacgcgc
     5941 tgctctgcat ccagacccag agctgcctca ccgacgagaa gcgcatgaag tacggcagcg
     6001 acgagttcta cctcaagtcg gccgaggaga tggaggagcg cttcagcgac taccccggcg
     6061 cgaccgccgc gaccgtcgag atcgccgagc gctgcaacgt cggcctggac ttcggcgact
     6121 acaagctgcc cacgtacccg gtacccgagg gcttcaccga ggacagctac ctgcgcgacc
     6181 tgtgcgagca gggcatcgcg cgccgctacg gtgccgagcc cgggcaggag gtcatcgagc
     6241 gcctcgactt cgagctcggg gtcatcggcg agatggggtt cgacgcctac ttcctcatcg
     6301 tctgggacta cgtcaagttc gccaaggaca acggcatcgc cgtgggaccc ggccgaggct
     6361 ccgccgccgg gtccatcgtc agctacgccc tcggcatcac cgacatcgac ccgctcaagt
     6421 acgacctgct gttcgagcgc ttcctcaacc cggggcgcaa gagcatgccc gacatcgaca
     6481 tggacttcag cgtggaccgc cgcggagagg tcatcgacta cgtggcgcgc aagtacggcc
     6541 gggacagggt cgcgcagatc atcaccttcg gcaccatggc ggcgcgcgcc gccgtgcgcg
     6601 acgccgcgcg cgtcatgggc aagccgtacg cggtcggcga caagatcgcc aagatgatcc
     6661 ccgagcaggc gccgccggcg agcttctcgg acgcgatgct gcccggctgc gagctcaagc
     6721 aggcctacga cgccgacgag gaggtcaagg aggtcgtcga cctcgccatg agcctcgagg
     6781 gcctcatccg caacgactcc atccacgccg ccgccgtggt gatcagcgac cggccgctca
     6841 ccgactacct gccgctgcag cagaagggcg aggccgagct cgtcacccag ttcgacatga
     6901 acgacgtctc caagctcggc ctgctcaaga tggacttcct cggcctgcgc aacctcgacg
     6961 tcatcgaggc cgcgctggtg atcatcgagg agacgcgcgg cgtgtgcatc gagatcgacc
     7021 ggctgccgct ggacgacgag aagacctaca agatgctggc ccgcggcgac tccaccggcg
     7081 tgttccagtt cgagagcccc ggcatgaagg aggcgctgcg cgacatcggc cctacggagt
     7141 tcgaggacct catcgccatc gtcgccctct accggccggg gccgatgcag tacatcccca
     7201 cgtacgcccg caacaagaag gacccggcct cggtcgtcta cgaccacgag tccctgcgcc
     7261 cgatcctgga gccgacccac ggcgtgacca tctaccagga gcagtacatg gccatcgccc
     7321 gccgggtcgg cgggttcagc cccgcgcagg cggacgacct gcgcaaggcc atcagcaaga
     7381 agaaccacac gctgatggcc accctcaagg agcccctcat gaagggcctc gccgagaagg
     7441 acgtaccgcc ggccgtggcc aacaagatgt gggccaactt cgaggccacc ggcgactact
     7501 cgttcaacaa gagccacgcc gcctgctacg cgatgatcag ctaccgcacc gcctggctca
     7561 aggccaacta cccggtcgag tacatggcgg ccctggtctc gagcgtcatg aacacgaagg
     7621 acaaggtgcc cttctacgtc aaccagtgtc acgagatggg catcgaggtg ctgcctccgg
     7681 acgtcaacac cagccaggtc gggttcaccg tcgtggaggg caagatccgc ttcgggatga
     7741 acgccgtgaa gggcgtcggc gtgggcgcca tcgaggcgat catcagggcg cgcgaggagg
     7801 gcgggccgtt cgcgtcggtc ttcgacttct gctcgcgggt cgactcccag atggccaaca
     7861 agcgggtcct cgaggcgctc atccgcgccg gcgccttcga cagcaccggc gacccgcgcc
     7921 gcgggatgat cgaggtgctg cccctggcga tgtccgacgg ggagcgcaag cgcaaggacg
     7981 cggccaacgg ccagggcgac ctcttcggcg cgatggactc cggggacacc ggccacccgc
     8041 tgcccacggt gccgcggtcc gagtgggacc agacgacgct gctgcgctac gagaaggagg
     8101 cgctcggcct gtacgtctcg gcgcacccac tccagggcct gcgcgagcag ttgcgcgacg
     8161 agatcgagat gccggtgagc gcgctcgaca acgccggcga cggcgcgatg acctggacgg
     8221 gcggcatcgt cgccaacgcc cagaagcgcg tcagcaagaa cggcggcgtg tggctctcgt
     8281 tccgcctcga ggacgtcgac ggcggcatcg agtgccgcgc ctggcccaag gtctacgagc
     8341 agttccgcga gctgctcgtg gaggacgcga tcatcaaggt caaggggcgc atcgagaagc
     8401 gggccgaggg cgacagcatg ctcatggcca tcgaggtcgt gccgttcaac ggcgtgagcg
     8461 agtaccaccc gctcacggtg gtcatcgact ccgcttgcgt ggccccgacg gtgatggacg
     8521 acctgcgccg ggtgctcacc gacttccccg gcacggtgcc ggtggtgctg cagatggtga
     8581 gcggggagca gcgcgccagg ctgcgcgtgg gcgacggtct gcgggtcgag cccgtcgccg
     8641 ggctgtacgc ggagctcaag gcgctgctcg gcgagagctg tatcggggtc ggctgaggcc
     8701 ggcgcgggga ggaggacgcg aggatgatcc ccgagggcat gcgcgacgtc cttccgcccg
     8761 agacgggccg gctgcgggcg gtggaagacc ggctccgcga ccggttcgcc gcctacggct
     8821 acggcgaggt gcgcacgccg tggctggagt tcgcggagac gctcgagtcg tccggcgacg
     8881 acacgctctc cgccggctac cgtctccacg acgagcaagg ccatcaactg atggtccgca
     8941 ccgacatgac cgtgcccgtg gcgcgcatgg cggccgaccg ctgcgacgac gacccgctgc
     9001 cgctccgctt cagctacgtc gccccgtgca tccgcccgtg ggcgccgcag cgcggccagg
     9061 acggggagtt ccagcaggcc ggcgccgaac tgctggggct ggactcggcc gaggccgacg
     9121 ccgagtgcgt caccctgctc tgcgacgcgc tcgcggggct cggcctgcgc gccttcacgg
     9181 tgacgctcgg ttccgccgcc taccagcgcg cgctcatcga ctccctgggg ctgccggacg
     9241 aggaccggga gacgttcacc gaggccctcg ccgaccggga ctacccgctg gtggagagcg
     9301 tcgccggcaa cgccgacgtg gagtacgacg ccctcaaggc cctctggcgt gtcctcgagc
     9361 tcagcggcgg ggaggagacg ctcggccggg cgcgcaagct cgcgcgcaac gaggtgatgg
     9421 aggcggcggt ggctcgcctc gcccgcgtgc gcgacctcgt ggaggaggcg ggccacggcg
     9481 atcgcctggc gttcgacttc ggcctcatgc aggacctcag ctactactcc ggcctgatcg
     9541 tcgaggcgta cgcacccggc gtcggcctgc ccgtggccac cggcggccgc tacgacgggc
     9601 tgctggcgcg gttcgagtgg gacatccccg gcgtgggctt cgccgtcgcc gtggaccgcg
     9661 ccgccgacgc cctcgacgag gccggcgtcg agctcgtgtc cgcgccggcc gccgtcccgt
     9721 tcgtcggagg gctggaggcg ccggcgcggg cggcggagct ccgccgcgcc ggcatcgccg
     9781 tactggcgct gcccgaggac gtccgcgccg tggcgccgcc gctgctgctg cgccacggcg
     9841 gctcgtacac gctgcgcctc gccgacggcc gcgaggtcac gggcggcccg ggcgagatcg
     9901 ccgaggcgct ggggtccggg tgaggatcgg cctcgcctgc gaggccggcg cgccgccggc
     9961 cgacgtcctc gggctgctcg aggccgccgg cttgccggcc gcttcgctgc gcggggaggc
    10021 cgccccggcg ctgctcccgc acccccgggg tgtgtggttc ctcgggtccg ccgcggacgt
    10081 gctgcgcgcc tgcgaccgcg gcgcgctgga cgccggcgtg gtcggcagcg acgagctgct
    10141 ggaggggcgc cacggggtcg ccgacctgct cgatctgcgc tgccgccgcg acgatctcgt
    10201 cttcgccgtc gccggacccg gcgggcggcc ggaccgcagg ctccgcgtcg ccacccggca
    10261 cccggaggcc gcgcggcggt acttcgccgc ggccggcctg cagccggagc tgatcgagct
    10321 ggacgcaccg gcgctggcgc cggcgctcgg cctggcggcc ggagtcgtgg agctgcgcgc
    10381 ccgcctgcag gcgggcgccg tggagccgcc cgtcctggtg gaacaggggg tcgtggccgc
    10441 ctgcagcgcc cgtctcgtgg ccgggcggtc cgcgcgtgtc ctgcgccgcg acgatgtcaa
    10501 cgcgctcgcg gacgcgctgc gagcggtcgt ggaggagaga tgaagcacct gccctggaac
    10561 gacgacgtcg ccgcgacggt cgcgcagctg cgggcgctgg cgccgccgcc cgcggaggtc
    10621 cgcaaggcgg cggaggcgat cgtcgccgac gtgcgcgccc gcggcgacgc cgcggtgcgc
    10681 gagcagacgg cgcggctcga ccgcgtcgag ctcgcggagc actacgccgt ccccgccgcc
    10741 gagatgcggg cgcggctcga ggcgctcaag cccgagctgc gtgcggcgct ggagacggcg
    10801 gcggccaaca tccgcgccta ccacgagcgc gaggccgtcc ggccctggcg ggagaccctg
    10861 gcccaaggcc aggtcgtcgg ccaggaggtc gtgcccttgg gcgccgccgg cctctacgct
    10921 cccggcggcc tcgccgacta cccgtcgtcg ctgctcatga cggcgatccc ggcccaggtg
    10981 gccggcgtcg agcgcatcgt cgtctgctcg ccgccccggc cgggcggcgg cgcggccgcc
    11041 ggtgtcgccg ccgcctgcgc cctcatcggc atcgagcacc tctatcccat cggcggcgcc
    11101 caggcggtgg ccgccatggc cctgggcacc gcctgtgtcc cgcgctgcga cgtcatcgcc
    11161 ggccccggca acgcctacgt caccgaggcc aagcgcctgc tcgtcggcga ggtgggcatc
    11221 gacagcctgg cgggtccgag cgaggtcctc atcatcgccg actcgtcggg acgtccggag
    11281 tggcttgccg ccgacctcct ggcgcaggcc gagcacggca tgggcgccat ggcctgcctc
    11341 gtcgacgtgg gcggcgcgct cgccggcccg gtggccgcgg ccgtcgaccg cctctccgcg
    11401 gagctgggca tcgccacgga caacgtggtc ctcgtcgcct gccccgaccg cggcgcggcc
    11461 ctgacgctca gcaacgcgtt cgcgcccgag cacctggaac tgcactgcga gggcgcgcgc
    11521 gagctgctcg ccggcgtgcg caacgccggc gccgtgttcg tcggtcccca cgccgccacg
    11581 gcgttcgccg actacgccgc cggcagcaac cacgtgctgc ccaccggcgg ctcggcccgc
    11641 ttcggccagg gcctctccgt cgacgctttt cgcaggcgca tggctgtggt agaactcgac
    11701 ggcccagccg ccgccgcgct cgcagcgcag gtggcgacca tcgccgacga ggagggcctg
    11761 cgcgcgcacg cgctgtccgc ccgcctccgg gccagtgagg agctgacatg atcgagcgcc
    11821 gcgccgaggt cgtccgcacc accaccgaga ccgacatctc cctgacgctc gacctcgacg
    11881 gcacgggcgc cgccgacgtc gtcaccggca tcggcttcct cgatcacatg ctcaccctgc
    11941 tggcgcacca cggagggttc gacctcaccg tccgcgcc
//
LOCUS       DASUMX010000076        24082 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1603240, whole genome shotgun sequence.
ACCESSION   DASUMX010000076 DASUMX010000000
VERSION     DASUMX010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 24082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24082
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1603240"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..896
                     /locus_tag="VFZ86_03500"
     CDS             <1..896
                     /locus_tag="VFZ86_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641387.1"
                     /translation="RSVAAIRYRTPSGRRFDSFALDIEASIVKDVALRNSRLRALSRR
                     IRGAVGGSYPLGAIIPSPAGMVMHPGYWPGFPYRALADLYDVFVPMGYYTYHGDGYAN
                     AYRDTRDNIRIIREQTGLPGVPIHVIAGDGARSSAGETSAYVRALRENGALGGSMYDW
                     ATTSEASWRQLRNVRFNPRQRPALPLALPAAAPLGYCGADRSHPKEAFYETAGQTGDR
                     VLRFRLYDVQADEVRLVVNWTDMGPLAAGPVKAWSRSRTVTLPAALLEPGARNVIGFV
                     ARGDYPAWRRWGVRDVSLEAP"
     gene            1023..1781
                     /locus_tag="VFZ86_03505"
     CDS             1023..1781
                     /locus_tag="VFZ86_03505"
                     /inference="COORDINATES: protein motif:HMM:NF016163.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HEX5641388.1"
                     /translation="MDPGPVVVGVDIAASRPCVAVAVRGGRTLEALSWTESDDSLAGD
                     RARLLDWIDGLQPVAVGVDAPQRPRRARGAAATRPRACDAALSHRRISIYQVPTRAVA
                     AAADGLYTWMEAGWEYFRELGRRGYEPPAPGTLPGVLGQAPAALEVYPHATFVTMLGG
                     TPPPKTSRAGLRVRVAALRGAGVAWDEYYDHDSLDALAAALTAWRFVQGRATPVGDDR
                     DGYIWLPVTEHELRPSYGRLAEREALAAARHAGG"
     gene            complement(1832..2701)
                     /gene="hisG"
                     /locus_tag="VFZ86_03510"
     CDS             complement(1832..2701)
                     /gene="hisG"
                     /locus_tag="VFZ86_03510"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEX5641389.1"
                     /translation="MTLKLGLPKGSLEHATIDLFRRAGFLITVAERSYFPAIDDPEIE
                     CMMVRAQEMARYVEDGALDCGLTGRDWVVENEATVHTVADLVYAKQSFGKVRWVLAVP
                     DASPVRAPADLEGRIVATELVGVTRRWLAERGVSAKVEFSWGATEAKLPELADAIVEV
                     TETGSSLRANKLRIVETVLESTTQLIANVGAWEDPEKRRKIEDVSMLLQGAMAALDKV
                     GLMLNVRTGDLDGVLAVLPALRKPTISHLSDAGWLAVNTILEESVVREIIPRLKEVGA
                     EGIVEFPLNKIVM"
     gene            2940..4781
                     /locus_tag="VFZ86_03515"
     CDS             2940..4781
                     /locus_tag="VFZ86_03515"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5641390.1"
                     /translation="MAAHPRSLSRLLVLLVVLALAAGLVWGLAGAFASSSPSPAGDTV
                     TLKVGWTTDPDNLNPFVGIEQSSYELYHVAYDFLTNYGDTYLETQPGLAESWTKSEDG
                     LTWTFKIRPGVMWSDGQPLTARDVAFSFNYQKDLELSAFLSALDGIATVTAPDDATVV
                     IECDKPKADILSMWVPIVPEHIWSQFATYDEATKYLNEPPIVGSGPFQVVEREKGKFI
                     RCVANEDYWGGKPKIDEIIFQVYKNQDTLAQDLKLGTIDLAINIPPAQVAALKAEEGL
                     ASEACSQKAFDYLSFNCYEGPSLGNPVLKDVKFRQALNWAVDKDKLTALAYQTYADPA
                     TSIFEVDFYDPDLDWHWEPPVDVAYGFDLEKARQALAGAGYEDTDGNGILNDPRNGGK
                     DIKLRLWSRNESAQSQVMGKLITGWWEELGLDIEYSVEDNGVLIDGGYNYDGDTYKPD
                     YDAYIWAWQPSGSDPGRRLGYFRSVQIENQNDACWSNEEYDALWEQQSEELDPQARKE
                     IAYQMQEILYEESPYIILTYPKLLQAWNTERWEGWRRIPQPNGAVAFISDNVSNYYNV
                     APVAADTATETSSGSSLTWVIVVVVAVVVVVAVVLLVLRRRPRAEEG"
     gene            complement(4788..5711)
                     /locus_tag="VFZ86_03520"
     CDS             complement(4788..5711)
                     /locus_tag="VFZ86_03520"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF017864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEX5641391.1"
                     /translation="MPKIVQHPEILVQARRGRTVESLHRACAALAGPDGEIVERWGDP
                     GMVTYWRSSAKPLQAQAWLADGTIERFGWGAEELAIMSASHEGLDFQAGLVRRMLTDL
                     GLSEQDLRCDGALKARHNCSGNHTGFLAACVHHGWDVPTYQRAEHPAQQAALAAFAAL
                     TGLDAATVATGVDGCGIVCYATPVTVAATTFALLPELLPEISAAMRARPELVEAPGWI
                     DTEVMRALPGATSKCGAEGLSCFSLPDGRGLAVKVVDGADRALEPAGLAALEAVLGPD
                     ALSDELQRLARPPITNDAGDVVGGLLAVVPE"
     gene            5815..6972
                     /locus_tag="VFZ86_03525"
     CDS             5815..6972
                     /locus_tag="VFZ86_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX5641392.1"
                     /translation="MQYRQFGKLDFKPSALGFGAMRLPVLSGEDGKPDFKRIDYPLAD
                     AMLHRAIEGGVNYVDTAWMYHEENCETWLGEALKGGYRERVKLATKMPVWDVKQPADF
                     DRILGIQMERLQTDHIDYYLLHSLDEAHWRCVVEQDQLAAAERALADGRIGHLGFSFH
                     GTSEDFAMILAATDLWEFVQIQYNYMDEEYQAGRRGLELAAGKGLGVIVMEPVRGGAL
                     AGHVPPPVQAVWDEAPVRRSAAAWALQWVWSHPEVSFLLSGMSTMEHVEENLRLADAS
                     RPGLLTGEELALVARARDLYRELSPIPCTSCRYCMPCPQGVAIPDVFELYNDAHMYDN
                     LPRQQMAYRMFFSDEERADRCTACGECVEKCPQGIAVPEWLEKAQSFLVPC"
     gene            complement(7006..8109)
                     /locus_tag="VFZ86_03530"
     CDS             complement(7006..8109)
                     /locus_tag="VFZ86_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641393.1"
                     /translation="MSLDSEGAVMTGESSTSTIRARSLLAVLAVVLAAGAATLAAPAA
                     AATAPRPFPVAIQIGGQAGPEIAGPMVVWTDNRNGNLDIYGRNLSTRTDHAITTNRAQ
                     QDNPSVTRYVTESGKVNYVAVWVDARNREGGTATDIYGRNITTRTTFVVARSAAVKWY
                     PEIVDHWVVWVEADDPAGPYRVKARDLAARKTYTLATSSVLSPVGIDRRTVGSRRVYT
                     AVYTTGKGNISARNVPAGDQFIVSQRSTFEWMPDISGDRVVWWETGGRVMLRNLKTGT
                     RRFVHLGARPRVDGELVTWDGGGHGGEFVVSYVAGAKVYVRNVARGTGVVAIAQKDLT
                     CLFPAVSGRRVVWESGPATRILSHIHIYGARLQ"
     gene            complement(8139..9143)
                     /locus_tag="VFZ86_03535"
     CDS             complement(8139..9143)
                     /locus_tag="VFZ86_03535"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX5641394.1"
                     /translation="MQQVTVQEQVEHATLGWRFAAVLVDTVVLFGLLIIAMMVYILVL
                     AAQGRIDPQDPAAVQALSQDLGVSDLTANVVVFGALFVYYAVLEGIFGASVGKLVFRM
                     RVVMADGSRPTSGAVIVRNLVRIPEAWLLYIPAGISCLASGRRQRLGDHVARTVVVRR
                     RLSVAGGGSTAGPPAPPVPAPPVYGQPVTPATPPGGAWASSPPAAPAVTTAPDTGEAL
                     AHLKTAALAARGAHLNYLRFSERELAAGTADPAAGYSEEYVGAWFTLADAVAALRAAW
                     DAATAAAVAAGRTLDEACANQADLAHLLRELTPYASAETDEEIHAAFLAVARWEASAP
                     "
     gene            complement(9229..9621)
                     /locus_tag="VFZ86_03540"
     CDS             complement(9229..9621)
                     /locus_tag="VFZ86_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014629188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HEX5641395.1"
                     /translation="MPSDQRLREPGYAERQAKLRFRDWRFRVRDILAAVNAVAAYTDG
                     MTFEEFVADARTRDAVVRNLMTMGESVRWIPDVILDAHPRVPWRTMRGVRNVVVHEYF
                     GIDDAILWETVRGDLPPLVPLLEDVLAS"
     gene            complement(9611..9907)
                     /locus_tag="VFZ86_03545"
     CDS             complement(9611..9907)
                     /locus_tag="VFZ86_03545"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX5641396.1"
                     /translation="MTSREALRRLNRNREKLRTHQVRHIAVFGSTARNQATANSDVDL
                     LVEFQPGAPVGLFAYVRLLDFLTNVMDTRIDLATPGALRPEMRDEIVAEAIRAF"
     gene            complement(9951..10700)
                     /locus_tag="VFZ86_03550"
     CDS             complement(9951..10700)
                     /locus_tag="VFZ86_03550"
                     /inference="COORDINATES: protein motif:HMM:NF014635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HEX5641397.1"
                     /translation="MPADHAPLPDPSRLLRELDLRTPLVGLYDAPDPEAFAPLVTPTS
                     SSGRGPCLFAFYKRWEKGETLHLTADAYGCGGCGRSLFGVQVRERDDFIDFLWKDEGL
                     RASRDLMAGWVDHAPTYRPEHANVLVGPLKPQLARYLKTITFWVNADQFCVLQLGAYH
                     HHAWGEPDPVTVPFGSGCSELVVPFRDLDRPEAVVGGTDIAMRDGLPPGLLAFTVTLP
                     LFAQLCALGDESFLGKGFLGRLRKARGGSLA"
     gene            complement(10784..13408)
                     /locus_tag="VFZ86_03555"
     CDS             complement(10784..13408)
                     /locus_tag="VFZ86_03555"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HEX5641398.1"
                     /translation="MRPLRVVMEAFGPYAAQQTLDFAELRGASFFLITGPTGSGKTTV
                     LDAMSFALYGVTSGGPESEGGRSGASMRSDHADPGLLTRVTFDFALGGDRYRIVREPE
                     QERPKLRGDGVTVHGQSATLWRLRQSSEGLAEDGGPLETGWSKVSARSEVLLGFRAEQ
                     FRQVVMLPQGRFQRLLAADSREREQILRALFDTGHYSAVEAELKRAAKGLSDAAKTLR
                     DKRAEALRQAEAETAEDLAERRARQALEAAEAAGHAGTAERARDEAQTRLQGGREAVA
                     RLDELAAAEAAAAAVAEREQLVAGARAELGAAEQAAAVSGAAARVAAARAGAAERRVA
                     AEEHEAERLRRAEAAAEAAAALEKLEGEAEDREAAAAECLRLKGLVGATADLAAAAAD
                     AGDADVVCGEARRVAAAADADWTAARDAAAEAEAAWRNAQAGHLAAGLSSGEPCPVCG
                     STDHPSPATLPARAPGQDEVEALRTAADRASAARDAATRSLLRAESTAAAAAACLQER
                     QKATAAEFADPAALQTAIATAEQRSAALVAAYRDASSAAQQTATAAATAATAAATSAA
                     ELVKADEEVRASCAELTERLSAAGFADEEAWLAACREPQQVGRLRELIADHAEAAARA
                     AERLRLARAAAEGVTTPDLEALETAALAAAAEALSARTAAVELAAAADTASRQLERLE
                     ELEREAAVVHERYEVIGRLADVANGDNPRHLSFQRYVLGAFLDDVLVAASQRLHLMTK
                     GRYRLERTERRFGGRSAAGLNLEVYDAWTGIARPVATLSGGESFMAALSLALGLAEVV
                     QAHAGGIHLETVFVDEGFGSLDDESLDLAIAALMGLGEDGRLVGIISHVSELRERIDA
                     RLEITAGKTGSRADFRVA"
     gene            complement(13405..14586)
                     /locus_tag="VFZ86_03560"
     CDS             complement(13405..14586)
                     /locus_tag="VFZ86_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018312840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HEX5641399.1"
                     /translation="MRLIHTADWHLGRLFHNVHLTRDQEHVLEQLVELARDVRPAAVV
                     VAGDLYDRAVPPTEAVDLLDHVLTELVDGLRVPVVAIAGNHDSGVRVGFAAALLRERG
                     LHLVGELPQAASPIVLRDEHGPVRICALPFADPAEARHVYGDEAIHDQQAVAAAGVRQ
                     ALAAAAPDERRVLVAHAFVAGGLESESERPLSVGGAQQVPAGVLDGFDYVALGHLHRP
                     QTCGSETTRYAGSLLKYSFAEAAHDKSVCVVDIGPRGGAPGETGRATVSVETVALSPR
                     RDVRRLEGTLAELLARGAADPRRDDYILASLTDDGALFDPIGRLRSAYPNALAIERPA
                     YERPGAGGWDRPRPGSVGDVDLFASFFGYVTDDELGDARREALTSVLDGLERARREAR
                     G"
     gene            14705..16099
                     /locus_tag="VFZ86_03565"
     CDS             14705..16099
                     /locus_tag="VFZ86_03565"
                     /inference="COORDINATES: protein motif:HMM:NF022525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HEX5641400.1"
                     /translation="MRTFRLSKSRITAGLQCGKRLWLAVHRPELEAYTADVQRRSAAG
                     HGIGEIARELYPGGVLIGDGAPLSQALRETEHALAQPGDVTLYEATFRHKGVLVRADV
                     IERRGGRCRMVEVKSATRLKEYHLQDIAIQGWVAEGAGVHLDGLSVAVVDTGFVYPGG
                     GDYRGLFREVPVADQARPLMAHIPGWVRGFNELLAGPLPARRIGPQCRRPFECPFLEF
                     CEGQEGKTRCAPGEERGEDGVCALPPDPALGSLDPAATAFLETLPYPRYYLDFETVQF
                     AVPLWAGTSPYQQIVFQWSCHEEERPGVVIHREFLDTSGESPTRRAAETLLEALGDEG
                     PIFVYHDFEKWRLMEMAAMLPDLAAELNAVAGRLIDLLRLTRDHYQHPALNGSYSLKN
                     VLPTVDAELDHALLEEVQDGLSAQAAYHEAVDPATPVERRDVLRASLLEYCGLDTLAL
                     VRVAHRFATAGAAS"
     gene            16096..16668
                     /locus_tag="VFZ86_03570"
     CDS             16096..16668
                     /locus_tag="VFZ86_03570"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEX5641401.1"
                     /translation="MTHLAEIDAGDGRLVPTLADILPEDGELRALFIGKTPSPASVEA
                     GHYFEGKMGRGLWKRLGDAGILSLPPGGHGDDALLAAGFGVTDLCKVPRPFGDEPGRE
                     EYEAGWERLSGVVARLRPRILVFVYKGSLDKVLQFSFGWEHKSGYGFNDDLARTFGRR
                     VFAFPLPGVSCTAREAQRHMTDLARAFEVL"
     gene            16683..17261
                     /locus_tag="VFZ86_03575"
     CDS             16683..17261
                     /locus_tag="VFZ86_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009181084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HEX5641402.1"
                     /translation="MKVVAFNGSARKDGNTAILVRRVFSELEAEGIETELVQLYNKGF
                     RGCTACLKCAETQDNTCSGLKDDGVNACIATMVEADGIIIASPTYYANCTATTQALLE
                     RAGYATRKSGNPLARKVGAAVVAARRGGAIHAFDSINHWFQINEMIVVGSSYWNLGIG
                     LDKGDVEQDDEGMQTMATLGRHMAWALKKLNA"
     gene            17269..17877
                     /locus_tag="VFZ86_03580"
     CDS             17269..17877
                     /locus_tag="VFZ86_03580"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HEX5641403.1"
                     /translation="MEPVSGGGAPPKVLLFDGSANPGGSIAALLERVREALGAEGVEA
                     ELARLERNRFTGCTMCGECGHRRDLGCARPPVDGLRRCARRLLAADGVVIGSPAYGAG
                     VSPATQRLMAHLEQRRRVHGEQPLAGKVAAAVVDVRSARAQHSLAVLTQWFHANGMIV
                     VGHAGGTRREGRPTAGQASEDAGAAMAALGRAVALTLERLRV"
     gene            complement(17987..18154)
                     /locus_tag="VFZ86_03585"
     CDS             complement(17987..18154)
                     /locus_tag="VFZ86_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641404.1"
                     /translation="MRADDRSQKGSAKKAGHPHERWQDPKRVTQPPKSWTNKSMHHPA
                     TRIGEIKHGKS"
     gene            complement(18225..18899)
                     /locus_tag="VFZ86_03590"
     CDS             complement(18225..18899)
                     /locus_tag="VFZ86_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013322970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HEX5641405.1"
                     /translation="MDVTFENGPAFTVAVAKLAPGEQVRCEGGAMLSMTTGTQIETST
                     QGGILKGLKRSISGESFFMNTFTAPAGGGEIVLAHSLPGDMSYIDQQGETWLYHRDGF
                     VASEMGIDLDTSWGGLKSAFGASSFFVVKCSGRGKVVYATYGALRRVDLQSGEQYTVD
                     SGHLVAWHEAMPVQVHKVGGMKSLFFSGEGFVVTLTGPGTFFTQTRSEQAFLNWIVPY
                     LPKSGS"
     gene            19212..19604
                     /locus_tag="VFZ86_03595"
     CDS             19212..19604
                     /locus_tag="VFZ86_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641406.1"
                     /translation="MARYVQRNQSDWFGTIWKLAIFVAVVLFAILVVYPWKGFLPTFL
                     LIVAGLWMYISLISRTSGYVCGKCGKAFQVPTTVNFFTQSAVGKNPDGTYYSYKNLTC
                     PHCGKQSKARLVKRVGAREAKGSGKLLR"
     gene            complement(19644..22505)
                     /locus_tag="VFZ86_03600"
     CDS             complement(19644..22505)
                     /locus_tag="VFZ86_03600"
                     /inference="COORDINATES: protein motif:HMM:NF024703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HEX5641407.1"
                     /translation="MPRPRPHFVVIDTETTGLDPRADRIIDIGAVRLDQDLRVSATFT
                     TLVDPGIPIPLFITRLTGISDADVAGAPRAADALADLRAFAGDATLVGHNAAFDREHL
                     AAAARCAGAAPLTGDWFDTLDAALLLYPELDRHALPILVEELGLEWRAHRALPDAQAT
                     AAVLALLARRAAGLADVERRLLESVAWAPLPVLDACAAAPDEAPPPLVADEPPEGPGP
                     LAVLPVDPDGWRAELDAGGGAVPGLAGRLPGFCRRAGQVEYGEAVAGVFGAGGIGLFE
                     AGTGMGKSLGYLLPAAFAGAATGRRVLVSTKTKALQRQLARTELPLVAAALPPGWRWA
                     LLMGRQNYLCRRRLDEAVAAEGEALPDRDRTLALAYLAGRARRGDVDLSALPFRATQV
                     LPALSDLARELRSSRATCLGRFCPARRGCHWRLARARADAAHLVCVNHALLLSGRDML
                     PAFEDLVIDEAHLLYHEATEAFSDRVDALSLDQLLGDVRGRHRQRPLPQRLRAVARRL
                     PPDEGRTLTAAADACERAAEEAPALSRGLGEAIAALAAAAAQTQDGADGGGRQADAYT
                     TTVWLTAGLRDLPAHDVFLTHAGLLAESLAKLAGAAAAGAEALPDDHRERSALIALAD
                     DAAAAAELLGDVPEGGGPETVAWAELESAARSAAGAARWALTRSPVTPAAGIREILWE
                     RLRSAVLTSATLTVAGSFAYFRDMTGLSGEVDVREHVFASPFDYRRQAVLVLEHDPGG
                     AWRPDELAGRQGERLKRIAEVTGGRTLALFTNTRDMHRVAAAVGEHVEDEGVLVLAQG
                     LHGSAASLADEFRTHPETILFGVDTLWTGQDFPGDALTCLVIAKLPFPRLDPLFRARR
                     RACEEAGERWFERFYLPEAVLKFRQGFGRLIRTESDTGVVVVLDHRLTQKSYRQDFLA
                     SLPEMDVVEAAPAEVAAAVDHHLRRLAAAAPAPPVGAGA"
     gene            complement(22579..23652)
                     /locus_tag="VFZ86_03605"
     CDS             complement(22579..23652)
                     /locus_tag="VFZ86_03605"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX5641408.1"
                     /translation="MYLSVQYAARRLGVSPHTIRRWTASGFLPCTRTAGGHRRIKQED
                     IDELAHLIGGRNHLAARLARERELETLVATAVAVSSQLDAGALLREIAKQMTTLLDAH
                     LCVISDYDPATRTVSVRAEYDDRGNRRQDTMRYTLGQFPMARRAIEHHESITVNVGDP
                     HADPAEVAIMRRDGDKCLLILPMVHQGESIGLIEVLDHQRERKFSRQEMRLANAVAAQ
                     AAVALHNATVFAQLTRSDQDALTLRHAIETIVAGYRTLHEQAGPAGVLQAAAEVSARA
                     LGAISCVASHAGASAGASGLRPADQALAQTGSARVIVAAAPCGETEVTLTTTLGSDAG
                     DGQAELLALVATMAAGAIERLPA"
     gene            complement(23845..>24082)
                     /locus_tag="VFZ86_03610"
     CDS             complement(23845..>24082)
                     /locus_tag="VFZ86_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641409.1"
                     /translation="SCVASLGGESAGAFGAGRVAAPSRADVARGANASLVVARDPSGR
                     TDLTLAVTLPHAPGEGQAELLDFVAGVAAVVAAG"
BASE COUNT         3219 a         8637 c         8981 g         3245 t
ORIGIN      
        1 ggcgcagcgt ggcggcgatc cgctaccgca cgccgtccgg ccggcgcttc gactcgttcg
       61 cgctcgacat cgaggcgagc atcgtcaagg acgtcgccct gcgcaacagc cggctgcggg
      121 cgctctcgag gcgcatccgc ggcgccgtgg ggggttcgta cccgctgggc gccatcatcc
      181 cgtcacccgc ggggatggtc atgcaccccg ggtattggcc cggctttccc taccgggcgc
      241 tggcggacct ctacgacgtg ttcgtcccga tgggctacta cacctaccac ggcgacggct
      301 acgccaacgc ctaccgcgac acgcgcgaca acatccgcat catccgcgag cagaccgggc
      361 tgccgggcgt gccgatccac gtgatcgccg gcgacggcgc caggtcctcg gccggcgaga
      421 ccagcgccta cgtgcgcgcc ctgcgcgaga acggcgccct gggcgggagc atgtacgact
      481 gggccacgac cagcgaagcg agctggcgcc agctgcgcaa cgtgcgcttc aacccgcgcc
      541 agcggccggc cctcccgctg gcgctgccgg cggccgcgcc gctcggctac tgcggcgcgg
      601 accgctcaca ccccaaggag gccttctacg agaccgccgg ccagacgggc gacagggtgc
      661 tgcgcttccg cctctacgac gtgcaggcgg acgaggtccg cctggtggtg aactggacgg
      721 acatgggccc gctggccgcc gggccggtca aggcgtggag ccggtcgcgc accgtgacgc
      781 tgccggcggc cctgctcgag ccgggggcgc gcaacgtcat cggcttcgtg gcccgcggcg
      841 actaccccgc ctggcggcgc tggggcgtcc gcgacgtcag cctggaagct ccgtgaggcc
      901 cgtcgcctga catccgccag cctggccggc ctgcgccttc cccggccggc ccgctccgtc
      961 tccatccgtc tggccggccg gtcaccttcc gccgcccggg cgccgtctgc tacgctgcga
     1021 ccatggatcc gggcccggtg gtcgtcggag tggacatcgc cgcgagcagg ccgtgcgtgg
     1081 ccgtcgccgt gcgcggcggc cgcacgctcg aggcgctctc gtggaccgag tcggacgaca
     1141 gcctcgccgg cgacagggcc aggctgctgg actggatcga cggcctgcag cccgtggccg
     1201 tgggcgtgga cgcgccgcag cggccgcggc gggcacgggg cgcggccgcc acgcggccgc
     1261 gggcctgcga cgcggcgctg tcgcaccggc gcatctcgat ctaccaggtg cccacgcgcg
     1321 ccgtcgccgc cgcggcggac ggtctgtaca cctggatgga ggcgggctgg gagtacttcc
     1381 gtgagctggg ccggcgcggc tacgagccgc cggccccggg gaccctcccg ggggtgctcg
     1441 gccaggctcc ggcggcgctc gaggtctacc cgcacgccac cttcgtcacc atgctcggcg
     1501 ggacgccgcc gcccaagacc tcgcgcgccg gcctgcgcgt gcgcgtcgcg gcgctgcgcg
     1561 gcgccggggt ggcgtgggac gagtactacg accacgactc gctggacgcg ctcgccgcgg
     1621 cgctcacggc gtggcgcttc gtgcagggac gcgccacccc cgtgggcgac gaccgcgacg
     1681 gctacatctg gctgccggtg accgagcacg agctgcggcc gtcgtacggc cggctcgccg
     1741 agcgggaggc cctggcggcc gcccgccacg ccggcggctg agcgctccgg ccccgccggc
     1801 ggctgagcgc tccggccccg ccggccgggc cctacatcac gatcttgttg agcgggaact
     1861 cgacgatgcc ctcggcgccc acttctttga gcctggggat gatctcgcgc accacgctct
     1921 cctcgaggat cgtgttcacc gccagccagc cggcgtcgct gaggtgcgag atcgtgggct
     1981 tgcgcagcgc gggcagcacg gcgagcacgc cgtcgaggtc gccggtgcgc acgttgagca
     2041 tcagcccgac cttgtcgagc gccgccatcg cgccctggag cagcatcgag acgtcctcga
     2101 tcttgcgccg cttctcgggg tcctcccagg cgccgacgtt ggcgatgagc tgggtcgtgc
     2161 tctcgagcac cgtctcgacg atgcgcagct tgttggcgcg cagcgagctg cctgtctccg
     2221 tgacctcgac gatggcgtcg gccagctccg gcagcttcgc ctcggtggcg ccccagctga
     2281 actccacctt ggcgctgacc ccacgctcgg cgagccaccg ccgggtgacg ccgaccagct
     2341 ccgtggcgac gatcctgccc tcgaggtcgg cgggcgcgcg caccggcgac gcgtcgggga
     2401 ccgcgagcac ccagcgcacc ttgccgaagc tctgcttggc gtagacgagg tcggcgacgg
     2461 tgtgcaccgt ggcctcgttc tcgaccaccc agtcgcgacc ggtgaggccg cagtcgagag
     2521 cgccgtcctc gacgtagcgc gccatctcct gggcgcgcac catcatgcac tcgatctcgg
     2581 ggtcgtcgat ggcggggaag taggagcgtt ccgcgaccgt gatcaggaag cccgcacggc
     2641 ggaagaggtc gatcgtcgcg tgctcgagac tccccttcgg caggccgagc ttgagtgtca
     2701 tgtccccctc cttccgtgga tcgctgaggc cgccgcacag tgcgccggcg ctgcatgacg
     2761 cgggcgcgag tgtatcgcag atgtgcggcc cggcgtcgga gcgcgcccgc cgcccgccgc
     2821 gctacctctt gcgcggcccg ggcgggattg catagcctgt cggggcggcg ccggggggcg
     2881 ccgcgcgacc tcccttggcg ggaggtcgcc aatgagctgc cgcgcagaaa ggatggccca
     2941 tggctgcgca tccgcgatcg ctgtctcgac tcctcgtcct gctggtcgtc ctggcccttg
     3001 ctgcgggcct cgtctggggg ctcgccggcg ccttcgccag ctccagcccg tcgccggccg
     3061 gcgacacggt caccctcaag gtgggctgga cgaccgaccc cgacaacctc aaccccttcg
     3121 tgggcatcga gcagtccagc tacgagctct accacgtcgc ctacgacttc ctcaccaact
     3181 acggcgacac gtacctcgag acccagcccg gcctggccga gagctggacc aagagcgagg
     3241 acggcctcac ctggaccttc aagatccgcc cgggcgtgat gtggagcgac gggcagccgc
     3301 tcacggcaag ggacgtcgcc ttcagcttca actaccagaa ggacctcgag ctgtccgcgt
     3361 tcctctcggc gctcgacggc atcgccaccg tgaccgcgcc cgatgacgcc acggtggtca
     3421 tcgagtgcga caagcccaag gccgacatcc tctcgatgtg ggtgcccatc gtgcccgagc
     3481 acatctggag ccagttcgcg acctacgacg aggccacgaa gtacctcaac gagccgccga
     3541 tcgtcggttc agggccgttc caggtggtcg agcgcgagaa gggcaagttc atccgctgcg
     3601 tggccaacga ggactactgg ggcggcaagc ccaagatcga cgagatcatc ttccaggtct
     3661 acaagaacca ggacacgctg gcgcaggacc tcaagctggg gaccatcgac ctggccatca
     3721 acatcccgcc ggcgcaggtc gcggcgctga aggccgagga ggggctcgcc tccgaggcgt
     3781 gctcgcagaa ggccttcgac tacctgtcgt tcaactgcta cgagggcccg tccctcggca
     3841 accccgtcct caaggacgtc aagttccgcc aggcgctcaa ctgggcggtg gacaaggaca
     3901 agctcaccgc cctcgcctac cagacgtacg ccgacccggc gacgtcgatc ttcgaggtgg
     3961 acttctacga cccggacctc gactggcact gggagccgcc ggtcgacgtg gcctacgggt
     4021 tcgacctcga gaaggccagg caggcgctcg ccggcgcagg ctacgaggac accgacggca
     4081 acggcatcct caacgacccc aggaacggcg gcaaggacat caagctgcgt ctctggtcgc
     4141 gcaacgaatc ggcgcagagc caggtgatgg gcaagctcat caccggctgg tgggaggagc
     4201 tgggcctcga catcgagtac tccgtcgagg acaacggcgt gctcatcgac ggcggctaca
     4261 actacgacgg cgacacctac aagccggact acgacgccta catctgggcg tggcagccga
     4321 gcggctcgga cccgggccgg cggctcggct acttccgcag cgtgcagatc gagaaccaga
     4381 acgacgcctg ctggtcgaac gaggagtacg acgcgctctg ggagcagcag tcggaagagc
     4441 tcgacccgca ggcgcgcaag gagatcgcct accagatgca ggagatcctc tacgaggagt
     4501 cgccgtacat catcctcacg taccccaagc tgctccaggc gtggaacacc gagcgctggg
     4561 agggctggcg gcgcatcccg cagcccaacg gcgccgtcgc cttcatcagc gacaacgtca
     4621 gcaactacta caacgtggcg ccggtggcgg cggacacggc gacggagacc tccagcggtt
     4681 ccagcctcac ctgggtcatc gtcgtcgtgg tggccgtggt cgtggtggtc gccgtcgtgc
     4741 tgctcgtcct ccggcgcagg cccagggccg aggagggctg agcgaggcta ctcggggacg
     4801 acggcgagca gtccgccgac gacgtcgccg gcgtcgttgg tgatcggcgg ccgcgccagg
     4861 cgttggagct cgtcgctcag ggcgtccgga ccgagcacgg cctcgagcgc ggcgagaccg
     4921 gcgggctcga gcgcgcggtc ggcgccgtcc acgaccttga ccgcgagccc gcgaccgtcc
     4981 ggcagcgaga agcagctcag gccctcggcg ccgcacttgc tggtggcgcc ggggagggcg
     5041 cgcatgacct ccgtgtcgat ccagcccggc gcctcgacga gctcgggacg cgcgcgcatc
     5101 gcggcgctga tctccgggag cagctcgggc agcagggcga aggtcgtggc ggcgacggtg
     5161 accggagtgg cgtagcagac gatgccgcag ccgtcgacgc cggtcgcgac ggtcgcggcg
     5221 tcgagcccgg tgagcgccgc gaacgccgcg agcgcggcct gctgcgcagg gtgctccgcc
     5281 cgctggtagg tcggcacgtc ccagccgtgg tgcacgcagg cggccaggaa cccggtgtgg
     5341 ttgccggagc agttgtggcg cgccttgagc gcgccgtcgc agcgcaggtc ctgctccgag
     5401 agcccgaggt ccgtcagcat gcggcgcacg aggccggcct ggaagtcgag gccctcgtgc
     5461 gaggcgctca tgatggcgag ctcttcggcg ccccagccga agcgctcgat ggtcccgtcg
     5521 gcgagccagg cctgggcctg cagcggcttg gccgacgagc gccagtaggt caccatgccc
     5581 gggtctcccc agcgctccac gatctcgccg tcgggtcccg ccagcgcggc gcaggcacgg
     5641 tgcaggctct ccacggtccg ccccctgcgg gcctggacga ggatctcggg gtgctggacg
     5701 atcttcggca tgcggctgct cctcgggacg actggcgccg agtctacagc cggcgtgccg
     5761 ccggcgcacc gccgaccggc gccgcgccgt cgttcaatcg ggtagcatgg cgccatgcag
     5821 taccgccagt tcggcaagct cgacttcaag ccctcggccc tcggcttcgg cgccatgcgc
     5881 ctgcccgtgc tctccggcga ggacggcaag ccggacttca agcgcatcga ctacccgctg
     5941 gcggacgcga tgctccaccg cgcgatcgag ggcggcgtga actacgtgga caccgcctgg
     6001 atgtaccacg aggagaactg cgagacgtgg ctcggcgagg cgctcaaggg cggctatcgg
     6061 gagcgggtca agctggccac caagatgccc gtctgggacg tcaagcagcc ggccgacttc
     6121 gaccgcatcc tcggcatcca gatggagcgg ctgcagacgg accacatcga ctactacctg
     6181 ctgcactcgc tcgacgaggc gcactggcgc tgcgtcgtcg agcaggacca gctcgccgcg
     6241 gccgagcgcg cgctcgccga cggccgcatc ggccacctcg gcttcagctt ccacggcacc
     6301 tccgaggact tcgccatgat cctcgccgcc accgacctct gggagttcgt ccagatccag
     6361 tacaactaca tggacgagga gtaccaggcc ggccgcaggg ggctcgagct ggcggccggc
     6421 aagggcctcg gcgtcatcgt catggagccg gtgcgtggcg gcgctctggc agggcacgtg
     6481 ccgccgccgg tgcaggcggt atgggacgag gcgcccgtca ggcggtcggc ggccgcgtgg
     6541 gcgctgcagt gggtctggag ccatccggag gtgtcgttcc tcctcagcgg catgagcacc
     6601 atggagcacg tggaggagaa cctcaggctg gccgacgcct cgcggcccgg cctgctcacc
     6661 ggggaggagc tcgcgctcgt ggcccgggcg cgcgacctgt accgcgagct cagccccatc
     6721 ccgtgcacgt cgtgccgcta ctgcatgccg tgcccccagg gcgtggcgat cccggacgtg
     6781 ttcgagctct acaacgacgc ccacatgtac gacaacctcc cgcgccagca gatggcgtac
     6841 cggatgttct tctccgacga ggagcgcgcc gaccgctgca cggcgtgcgg cgagtgcgtg
     6901 gagaagtgcc cgcagggcat cgccgtgcct gagtggctcg agaaggcgca gtccttcctc
     6961 gtcccctgct gacggcggcg ccggcgagcg cgccgccgcg gccggtcact gcagtcgcgc
     7021 tccgtagatg tggatgtggc tgaggatcct cgtcgccggt cccgactccc acaccacgcg
     7081 ccggccgctg acggcgggga agaggcaggt caggtccttc tgggcgatgg cgaccacgcc
     7141 cgtgccgcgc gcgacgttgc gcacgtagac cttggcgccg gcgacgtagg agacgacgaa
     7201 ctcgcccccg tgcccgccgc cgtcccaggt caccagctcg ccgtcgacgc gcggccgcgc
     7261 cccgaggtgc acgaacctgc gcgtgcccgt cttgaggttg cggagcatga cccgcccgcc
     7321 ggtctcccac cacaccacgc gatcgccgct gatgtcgggc atccactcga aggtgctgcg
     7381 ctgcgagacg atgaactggt cgccggcggg gacgttgcgg gcgctgatgt tgcccttgcc
     7441 ggtcgtgtag acggcggtgt agacgcggcg cgacccgacg gtgcggcggt cgatgccgac
     7501 ggggctgagc acgctgctgg tggccagggt gtaggtcttc cgcgcggcga ggtcgcgcgc
     7561 cttgacgcgg tacgggccgg cggggtcgtc cgcctccacc cagaccaccc agtggtccac
     7621 gatctccggg taccacttca cggcggcgct gcgcgccacc acgaaggtgg tgcgggtggt
     7681 gatgttgcgc ccgtagatgt cggtcgccgt gccgccctcg cggttgcgcg cgtcgaccca
     7741 cacggcgacg tagttgacct tgcccgactc ggtgacgtag cgggtgacgg acgggttgtc
     7801 ctgctgcgcc ctgttcgtgg tgatcgcgtg gtccgtgcgc gtgctgaggt tgcggccgta
     7861 gatgtcgagg ttgccgttgc ggttgtcggt ccagacgacc atcggcccgg cgatctccgg
     7921 tccggcctga ccgccgatct ggatggcgac ggggaagggc cgtggggcgg tcgcggcggc
     7981 ggccggcgcc gccagggtcg ccgcccccgc ggccaggacg accgccagga cggcgagcaa
     8041 ggagcgcgcc cggatggtgc tggtagacga ctccccggtc atgaccgccc cctcgctgtc
     8101 gagagacact gcagtcatcg taccccgggc gtccagcgtc agggggcgga tgcctcccag
     8161 cgggcgacgg ccaggaacgc ggcgtggatc tcctcgtcgg tctcggcgct cgcgtacggc
     8221 gtgagctcgc gcaggaggtg ggcgaggtcg gcctgattgg cgcaggcctc gtccagcgtg
     8281 cgccccgccg cgacggcggc ggccgtcgcc gcgtcccagg cggcgcgcag tgcggcgacg
     8341 gcgtcggcga gcgtgaacca ggcgccgacg tactcctccg agtacccagc cgccgggtcc
     8401 gccgtgccgg cggcgagctc gcgctcggag aagcgcaggt agttgaggtg ggcgccgcgc
     8461 gcggccagag ccgccgtctt gaggtgcgcg agcgcctcgc cggtgtccgg cgcggtcgtg
     8521 accgcggggg cggcgggagg agacgacgcc caggcgccgc cgggcggagt cgccggcgtg
     8581 accggctgcc cgtagacggg cggcgcggga acgggcgggg ccggcgggcc ggcagttgag
     8641 ccgccgccgg ccacggagag gcgacggcgc accaccacgg tgcgcgccac gtgatcgccc
     8701 agccgctgcc gccgtccgct cgccagacag gagatgccgg ccgggatgta cagcagccac
     8761 gcctcgggga tgcgcacgag gttgcgcacg atcacggccc cactcgtggg cctggagcca
     8821 tcggccatga cgacgcgcat gcggaagacg agcttgccga cgctggcgcc gaagatgccc
     8881 tcgagtaccg cgtagtagac gaacagcgcg ccgaagacga cgacgttggc cgtgaggtcg
     8941 gacacgccga ggtcctggct gagggcctgg accgcggcgg ggtcctgcgg gtcgatgcgg
     9001 ccctgcgccg cgagcacgag gatgtagacc atcatggcga tgatgaggag gccgaacagc
     9061 accacggtgt cgacgaggac ggccgcgaag cgccagccga gagtcgcgtg ttccacctgt
     9121 tcctggacgg tgacctgctg catcggcgcc gattctacac ggcgcggccg gagcgcgacg
     9181 ccggggaccg gtcagggggt ggggacggcg cccggcctgg gcgccgtctc aggacgcgag
     9241 gacgtcctcg agcaggggca cgagcggcgg caggtcgccg cgcacggtct cccagaggat
     9301 cgcgtcgtcg atgccgaagt actcgtgcac gacgacgttg cgcacgccgc gcatggtgcg
     9361 ccaggggacg cgggggtgcg cgtccaggat gacgtccggg atccagcgca ccgactcgcc
     9421 catggtcatg aggttgcgga ccaccgcgtc gcgcgtgcgc gcgtcggcga cgaactcctc
     9481 gaaggtcatc ccgtccgtgt aggccgccac cgcgttgacg gcggcgagga tgtcgcgcac
     9541 gcggaagcgc cagtcgcgga agcgcagctt ggcctggcgc tcggcgtacc ccggctcgcg
     9601 gaggcgctgg tcagaaggca cggatcgcct ccgccacgat ctcgtcgcgc atctcggggc
     9661 gcagggcgcc cggcgtggcg aggtcgatgc gcgtgtccat gacgttggtg aggaagtcga
     9721 ggaggcgcac gtaggcgaag agaccgacgg gcgcgccggg ctggaactcg accaggaggt
     9781 ccacgtcgct gttcgcggtc gcctgattgc gcgccgtgga cccgaacacg gcgatgtggc
     9841 gcacctggtg cgtgcgcagc ttctcgcggt tgcggttcag gcggcggagc gcctctcgag
     9901 aggtcatgga cctatgctac cggacgcggc cgggcgcagc cgcgtgcggc ctacgcgagc
     9961 gacccgccgc gcgccttgcg caggcggccg aggaagcctt tgccgaggaa gctctcgtcg
    10021 cccagggcgc acagctgcgc gaagaggggc agcgtgaccg tgaacgcgag gaggcccggc
    10081 ggcagcccgt cgcgcatcgc gatgtcggtg ccgccgacga ccgcctccgg ccgatccagg
    10141 tcccggaacg ggacgacgag ctcggaacag cccgatccga agggcaccgt gaccgggtcg
    10201 ggctcccccc acgcgtggtg gtggtaggcg ccgagttgca gcacgcagaa ctggtcggcg
    10261 ttgacccaga acgtgatcgt cttgaggtac cgggccagct ggggcttgag cggtccgacc
    10321 aggacgttgg cgtgctcggg ccggtacgtc ggcgcgtggt cgacccagcc ggccatgagg
    10381 tcgcggctcg cgcgcaggcc ctcgtccttc cagaggaagt cgatgaagtc gtcgcgctcg
    10441 cgcacctgga ccccgaacag cgagcgcccg cagccgccgc agccgtaggc gtcggcggtg
    10501 agatgcagcg tctcgccctt ctcccagcgc ttgtagaagg cgaacagaca gggtccccgc
    10561 cccgaactgc tcgtgggcgt cacgagcggc gcgaacgcct cggggtccgg ggcgtcgtag
    10621 agcccgacga gcggcgtgcg caggtcgagc tcgcggagca ggcgcgacgg atcggggagc
    10681 ggggcgtggt ctgcgggcat ggagcctccc tggactgcgg gcgagtggac ggcggccgtg
    10741 gccggttggc gcgattgtac gccggtcagg ccgctccggc cgctcaggcg accctgaagt
    10801 cggccctgct gccggtcttg ccggccgtga tctccaggcg cgcgtcgatg cgctcccgca
    10861 gctcgctcac gtgcgagatg atgccgacca ggcggccgtc ctcgccgagc cccatgaggg
    10921 cggcgatcgc cagatcgagc gactcgtcgt cgagggagcc gaagccctcg tcgacgaaca
    10981 cggtctcgag gtggatgccg ccggcgtgcg cctggaccac ctccgccagg cccagcgcca
    11041 gcgagagcgc cgccatgaag ctctcgccgc cggacagcgt cgcgacgggc cgggcgatgc
    11101 ccgtccaggc gtcgtagacc tcgaggttga gcccggcggc cgaccgcccc ccgaaacggc
    11161 gctcggtgcg ctcgagccgg tagcgcccct tggtcatcag gtgcagccgc tgcgaggcgg
    11221 cgacgagcac gtcgtcgagg aacgcgccca gcacgtagcg ctggaaggac aggtggcgcg
    11281 ggttgtcgcc gttggcgacg tcggcgaggc ggccgatgac ctcgtagcgc tcgtgcacga
    11341 ccgcggcttc gcgctcgagc tcctcgaggc gctcgagctg gcgggaagcc gtgtcggctg
    11401 ccgcggcgag ctcgaccgcg gcggtgcgcg cggagagcgc ctccgccgcc gccgcgagcg
    11461 ccgcggtctc gagggcctcg agatcgggcg tggtgacacc ttcggcggcg gcgcgcgcga
    11521 gccgcagccg ctccgccgcc cgtgcggccg cctcggcgtg gtcggcgatg agctcgcgca
    11581 ggcgcccgac ctgctgcggc tcgcggcagg ccgcgagcca ggcctcctcg tcggcgaagc
    11641 ccgccgccga gagacgctcg gtgagctcgg cgcaggaggc gcggacctcc tcgtccgcct
    11701 tgacgagctc ggcggccgac gtggcggcgg ccgtggccgc cgtggcggcg gcggtggccg
    11761 tctgctgggc cgcgctcgag gcgtcgcgat acgcggccac gagcgcggcc gagcgctgct
    11821 cggctgtggc gatcgccgtc tgcagcgccg ccgggtcggc gaactcggcc gcggtcgcct
    11881 tctggcgctc ctgcaggcag gccgccgcgg ccgccgccgt gctctctgcc cgcagcaggc
    11941 tcctggtcgc ggcgtcgcgc gccgccgacg cgcggtcggc ggccgttctg agcgcctcga
    12001 cctcgtcctg gcccggggct cgtgcgggca gtgtcgcggg ggaggggtgg tcggtggaac
    12061 cgcacaccgg gcagggctca cccgacgaca agccggcggc gaggtggccc gcctgggcgt
    12121 tgcgccaggc ggcctcggcc tcggccgcgg cgtcgcgcgc ggccgtccag tccgcgtcgg
    12181 cagcggccgc gacgcgacgc gcctcgccgc agaccacgtc ggcgtcgccg gcgtcggccg
    12241 ccgcggccgc gaggtcggcg gtcgcgccga cgagtccctt gaggcgcagg cactcggccg
    12301 ccgccgcctc cctgtcctcg gcctcgccct cgagcttctc cagcgctgct gccgcctcgg
    12361 cggccgcctc cgcgcggcgc aggcgctcgg cctcgtgctc ctccgccgca acgcggcgct
    12421 ccgcagcgcc ggcgcgcgct gcggccacgc gcgcggcggc tccgctcacg gccgccgcct
    12481 gctcggcggc gccgagctcc gccctggcgc cggcgacgag ctgttcgcgc tccgcgacgg
    12541 ccgcggccgc ggcctccgcg gccgccagct catcgagacg ggcgacggcc tcgcgcccgc
    12601 cctgcagccg cgtctgcgcc tcgtcccgcg cgcgctccgc cgtcccggcg tggccggcgg
    12661 cttcggcggc ctcgagcgcc tggcgcgcgc gtcgctcggc cagatcctcg gccgtctccg
    12721 cctcggcctg ccgcagcgcc tcggcgcgct tgtcccgcag cgtcttcgcc gcgtcgctca
    12781 gccccttggc cgctctcttg agctcggcct ccacggcgga gtagtggccg gtgtcgaaca
    12841 gcgcccggag gatctgctcg cgctctctgg agtcggcggc gaggagcctc tggaagcgac
    12901 cctgcggcag catgaccacc tggcggaact gctcggcgcg gaaccccagc aggacctcgg
    12961 acctggcgct gaccttggac caccccgtct cgagaggccc gccgtcctcc gcgaggccct
    13021 cgcttgactg gcgcagccgc cacagcgtgg ccgactggcc gtggacggtg acgccgtcgc
    13081 ctctgagctt gggtcgctcc tgctccggct cgcggacgat gcggtagcgg tcgccgccga
    13141 gcgcgaagtc gaacgtcacc cgggtgagca ggccggggtc ggcgtggtcg ctgcgcatcg
    13201 aagcgcccga gcgcccgcct tcgctctcgg gcccgccact tgtgacgccg tagagcgcga
    13261 acgacatggc gtcgaggacg gtggtcttac ccgaccctgt ggggccggtg atgaggaaga
    13321 agctggctcc ccgcagttcc gcgaagtcca gcgtctgctg cgcggcatac gggccaaacg
    13381 cctccatgac gacccgcaga ggcctcaccc gcgggcctcc cggcgggcgc gctcgagacc
    13441 gtcgaggacg ctggtgaggg cctcgcggcg cgcgtccccg agctcgtcgt cggtgacgta
    13501 gccgaagaag gacgcgaaga ggtcgacgtc gccgaccgac ccgggacgcg gtctgtccca
    13561 tcccccggcg ccggggcgct cgtaggccgg gcgctcgatg gcgagcgcgt tgggatacgc
    13621 ggagcgcagc cggccgatgg ggtcgaagag ggcgccgtcg tcggtgagcg aggccaggat
    13681 gtagtcgtcg cggcgcgggt ccgcggcccc gcgcgcgagc agctccgcca gcgtgccctc
    13741 gaggcggcgg acgtcgcggc gcgggctgag cgcgacggtc tcgacggaga ccgtcgcgcg
    13801 cccggtctcg ccgggagcgc cgccgcgcgg gccgatgtcg accacgcaca ccgacttgtc
    13861 gtgggcggcc tcggcgaagg agtacttgag cagagagccg gcgtagcgcg tcgtctcgga
    13921 gccgcacgtc tgggggcggt ggagatggcc cagggcgacg tagtcgaagc cgtcgagcac
    13981 cccggcgggc acctgctggg cgccgccgac gctgaggggc cgctcgctct cgctctcgag
    14041 gcctccggcc acgaacgcgt gggcgacgag cacgcggcgc tcgtccggcg cggcggcggc
    14101 gagcgcctgc cgtacgccgg cggcggccac ggcctgctgg tcgtggatgg cctcgtcgcc
    14161 gtacacgtgg cgcgcctcgg cgggatcggc gaacgggagc gcgcagatgc gcaccgggcc
    14221 gtgctcgtcg cgcagcacga tgggcgaggc cgcctgcggc agctcgccca ccaggtgcag
    14281 gccgcgctcg cgcagcagag cggcggcgaa gccgacgcgc acgccgctgt cgtggttgcc
    14341 ggcgatggcc accaccggga cgcggaggcc gtcgacgagc tcggtgagca cgtggtccag
    14401 gaggtccacg gcctcggtgg gcggcacggc gcggtcgtac aggtcgccgg cgacgacgac
    14461 cgcggccggg cgcacgtccc tggccagctc gaccagctgc tccagcacgt gctcctggtc
    14521 gcgcgtgagg tggacgttgt ggaagagccg cccgaggtgc cagtcggccg tgtggatgag
    14581 cctcatggcg gcgaccctac cacagcgtcc ggacggcccc cgggctcgtc cccgggaccc
    14641 ggcgcggtct cccggggagg ctccccggcg gcccgccgcc ccacagcggg atacaatagg
    14701 gggagtgcgc accttccgcc tctccaagtc ccgcatcacc gccggcctcc agtgtggcaa
    14761 acggctctgg ctcgccgtgc atcgtccgga gctggaggcg tacaccgccg acgtccagcg
    14821 acgctccgcg gccgggcacg gcatcggcga gatcgcgcgc gagctgtacc cgggcggcgt
    14881 gctcatcggc gacggcgcgc ccctcagcca ggcgctgcgc gagacggagc acgcgctcgc
    14941 acagccgggc gacgtcaccc tctacgaggc cacgttccgc cacaagggcg tgctggtccg
    15001 cgccgacgtg atcgagcggc gcggcggccg ctgccgcatg gtggaggtca agtcggcgac
    15061 ccgcctcaag gagtaccacc tgcaggacat cgccatccag gggtgggtgg cggagggcgc
    15121 cggcgtgcat ctggacggcc tgagcgtggc cgtcgtcgac accggcttcg tgtaccccgg
    15181 cggcggcgac taccgcgggc tgttccgcga ggtccccgtc gccgaccagg cgcgcccgct
    15241 catggcgcac atccccggct gggtgcgcgg cttcaacgag ctgctcgccg gtccgctccc
    15301 ggccaggcgc atcgggcccc agtgccggcg gccgttcgag tgcccgttcc tggagttctg
    15361 cgaggggcag gagggcaaga cgcgctgcgc gccgggagag gagcgcgggg aggacggcgt
    15421 ctgcgcgctc ccgcccgatc cggccctggg cagcctcgac ccggcggcga ccgcgttcct
    15481 ggagacgctg ccgtacccgc gctactacct cgacttcgag acggtgcagt tcgccgttcc
    15541 cctctgggcc ggcacgagcc cctaccagca gatcgtcttc cagtggtcct gccacgagga
    15601 ggagcggccc ggcgtggtca tccaccggga gttcctcgac acgagcggcg agtcgccgac
    15661 gcgccgtgcc gcggagacgc tgctggaggc tctcggggac gagggtccca tcttcgtcta
    15721 ccacgatttc gagaagtggc ggctgatgga gatggcggcg atgctgcccg acctcgcggc
    15781 ggagctcaac gccgtcgccg gccggctgat cgacctgctt cgcctgacgc gcgaccacta
    15841 ccagcatccg gccctcaacg ggtcgtactc gctcaagaac gtgctgccga ccgtggacgc
    15901 ggagctggac cacgcgctgc tcgaggaggt ccaggacggg ctctccgccc aggccgccta
    15961 ccacgaggcc gtcgacccgg cgaccccggt cgagcgccgc gacgtcctgc gggcctcgct
    16021 cctcgagtac tgcggcctcg acaccctggc gctggtgcgc gtggcgcacc ggttcgcgac
    16081 ggcgggggcg gcgtcgtgac gcatctcgcc gagatcgacg ccggggacgg ccgcctcgtg
    16141 ccgacgctcg ccgacatcct cccggaggac ggcgagctcc gcgccctgtt catcggcaag
    16201 accccgtcac cggcgagcgt cgaggccggc cactacttcg agggcaagat gggccggggg
    16261 ctgtggaagc ggctcgggga cgccggcatc ctcagcctgc cgccgggcgg ccacggcgac
    16321 gacgccctcc tggcggccgg cttcggcgtc accgacctgt gcaaggtccc gcggccgttc
    16381 ggggacgagc ccggccgcga ggagtacgag gccgggtggg agcgcctgtc cggcgtcgtc
    16441 gccaggctgc ggccgcgcat cctcgtgttc gtgtacaagg ggtcgctcga caaggtgctc
    16501 cagttctcct tcggctggga gcacaagagc ggctacggct tcaacgacga cctggcgcgg
    16561 acgttcgggc gccgggtgtt cgcgttcccg ctgcccggcg tgtcctgcac ggcgcgtgag
    16621 gcgcagcggc acatgaccga cctcgcccgc gcgttcgagg tcctttgagg gggaagaccg
    16681 acatgaaagt ggtggcgttc aacggcagcg cgcgcaagga cggcaacacg gccatcctgg
    16741 tgcgccgggt gttctcggag ctcgaggccg agggcatcga gaccgagctc gtgcagctgt
    16801 acaacaaggg ctttcgcggg tgcaccgcgt gcctcaagtg cgcggagacc caggacaaca
    16861 cctgttccgg gctcaaggac gacggagtca acgcgtgcat cgccacgatg gtcgaggcgg
    16921 acggcatcat catcgcctcg cccacctact acgccaactg cacggccacg acccaggcgc
    16981 tgctggagcg cgccgggtac gccacgcgca agagcggcaa cccgctggcg cgcaaggtgg
    17041 gcgcagcagt ggtcgccgcc aggcgcggcg gcgccatcca cgcgttcgac agcatcaacc
    17101 actggttcca gatcaacgag atgatcgtgg tcggctcgag ctactggaac ctgggcatcg
    17161 gcctcgacaa gggcgacgtg gagcaggacg acgagggcat gcagaccatg gccaccctcg
    17221 gccgccacat ggcgtgggcg ctgaagaagc tgaacgcctg acgctcccgt ggagcctgtg
    17281 agcggcggcg gggcgccgcc gaaggtcctc ctcttcgacg ggagcgccaa cccgggcggg
    17341 agcatcgccg cgctcctgga gcgtgtgcgc gaggccctcg gggccgaggg cgtggaggcc
    17401 gagctggcgc gcctcgagcg caaccgcttc accggttgca ccatgtgcgg cgaatgcggg
    17461 caccggcgtg acctgggctg cgcccgcccg ccggtggacg ggctcaggcg ctgcgcccgc
    17521 aggctgctcg ccgccgacgg cgtcgtgatc ggctcaccgg cctacggcgc cggagtgagc
    17581 cctgccacgc agcggctcat ggcgcacctg gagcagcggc gccgggtcca cggcgagcag
    17641 ccgctggcgg gcaaggtcgc ggcggccgtc gtggacgtgc gcagtgcgcg ggcgcagcac
    17701 tcgctggccg tcctcacgca gtggttccac gccaacggga tgatcgtcgt cgggcatgcc
    17761 ggagggacac gcagggaggg ccggccgacg gccggtcagg cgagcgagga cgcgggcgcg
    17821 gccatggctg ccctcggccg cgccgtggcg ctcacgctcg agcggctgag ggtgtagggc
    17881 cgggcggacg cggcggcgcc cccgcgcccg tacgcaccag ggcccggagg ccgcttcgac
    17941 ctccgggccc tcactcacga ccctcggcgg cggtccactg ccgcggtcac gacttcccgt
    18001 gcttgatctc gccgatccgg gtcgcggggt ggtgcatgga cttgttggtc caggacttgg
    18061 ggggttgggt gacccgcttg gggtcttgcc agcgctcgtg cgggtgcccc gccttcttcg
    18121 cggagccctt ctggctgcgg tcgtccgctc tcatctccga cctccgccga cgacgatcct
    18181 acccgggtgg tacccgtccg gcccgcgccc taacgccgtc cggctcagct gccgctcttg
    18241 ggcaggtacg ggacgatcca gttcaggaag gcctgctcgc tgcgggtctg cgtgaagaag
    18301 gtgccgggtc cggtgagcgt gaccacgaag ccttcacccg agaagaagag cgacttcatc
    18361 ccgccgacct tgtgcacctg gaccggcatc gcctcgtgcc acgccaccag gtggccgctg
    18421 tcgaccgtgt actgctcgcc ggactgcagg tcgacgcggc gcagcgcgcc gtaggtcgcg
    18481 tagaccacct tgccgcggcc gctgcacttg acgacgaaga acgacgaggc gccgaacgcg
    18541 ctcttgaggc cgccccaact ggtgtcgagg tcgatgccca tctccgaggc cacgaagccg
    18601 tcgcggtggt acagccacgt ctcgccctgc tggtcgatgt aggacatgtc accgggcagg
    18661 gagtgagcga gcacgatctc cccgccgccg gcgggggccg tgaaggtgtt catgaagaac
    18721 gactctccgg agatcgaccg tttgagcccc ttgaggatgc ccccttgcgt cgacgtctcg
    18781 atctgcgttc ccgtggtcat gctcagcatg gcgccgccct cgcagcggac ctgctctccc
    18841 ggcgccagct tcgccaccgc gacggtgaac gcggggccgt tctcgaacgt gacgtccatg
    18901 ttgttcctcc tggtcggtcc agcaaccgta cccgccgggc ttgagcggca gacctgtttg
    18961 cgggcatact cacaccctga cgcccgtccc ctgaggcggg cccggccggc acgactgcga
    19021 cgggagtcca catgtccaga gggttgttcg cggggttctt ccagaccttg gggttcgtcg
    19081 tcatggtcct cgtggcgctg ttcggcatct tcaccctggt cagctacctg gccggaccgg
    19141 tcggagcctg agaccggccg cggcggcgcc ccgctgcgcg ccgcccgcag cgacacagga
    19201 aaggaatccg cgtggcccgt tacgtgcaac gcaaccagtc agactggttc ggtaccatct
    19261 ggaagctcgc catcttcgtc gccgtcgtcc tgttcgcgat cctcgtggtg tatccgtgga
    19321 agggcttcct gcccacgttc ctcctcatcg tcgccgggct ctggatgtac atcagcctga
    19381 tctcgcgcac gtcgggatac gtgtgcggca agtgcggcaa ggcgttccag gtgcccacca
    19441 cggtcaactt cttcacgcag tcggcggtcg gcaagaaccc cgacggcacc tactacagct
    19501 acaagaacct cacgtgcccc cactgcggca agcagagcaa ggcgcggctg gtcaagcgcg
    19561 tcggggcgcg cgaggccaaa ggcagcggga agctgctgcg gtaggccggc ggtaagccgc
    19621 cgaggcagcc ggcggccggc gcgtcaggcg ccggcgccga ccgggggcgc cggggcggcc
    19681 gcggcgagcc gccgcaggtg gtggtccacc gcggcggcga cctccgcggg cgccgcctcc
    19741 accacgtcca tctccggcaa gctcgccagg aagtcctgcc ggtagctctt ctgcgtcagg
    19801 cgatggtcga gcaccaccac cacgccggtg tcgctctcgg tgcggatgag gcggccgaag
    19861 ccctgccgga acttgagcac ggcctccggc aggtagaagc gttcgaacca gcgctcaccg
    19921 gcctcctcgc aggcgcgccg ccgggcgcgg aacagcgggt cgagccgcgg gaacggcagc
    19981 ttcgcgatca ccaggcaggt gagcgcgtca ccggggaagt cctgcccggt ccacagcgtg
    20041 tcgacgccga acaggatcgt ctccgggtgg gtgcggaact cgtcggccag gctcgcagca
    20101 ctgccgtgga ggccttgggc gagcacgagc acgccctcgt cctccacgtg ctcgcccacc
    20161 gccgccgcga cccggtgcat gtcccgcgtg ttggtgaaca gggcgagtgt gcgcccgccg
    20221 gtgacctcgg cgatgcgctt gagccgctcg ccctggcggc cggcgagctc gtcgggccgc
    20281 caggcgccgc cgggatcgtg ctcgagcacg agcaccgcct gccggcggta gtcgaacggc
    20341 gaggcgaaca cgtgctcgcg cacgtcgacc tcgccgctca ggcccgtcat gtcgcggaaa
    20401 tacgcgaacg atcccgccac ggtgagcgtc gcgctcgtga gcacggcgct gcgcagccgc
    20461 tcccagagga tctcccggat gcccgcggcc ggagtgaccg gcgagcgggt gagcgcccag
    20521 cgcgccgcgc ctgccgcgct ccgggcggcg ctctcgagct cggcccacgc gaccgtctcg
    20581 gggccgccgc cctcgggcac gtcgccgagc agctccgccg ccgccgcggc gtcgtcggcg
    20641 agcgcgatca gcgcggaccg ttcgcggtgg tcgtcgggca gggcctcggc gccggccgcc
    20701 gccgcgccgg ccagcttggc gaggctctcg gcgagcaggc cggcgtgcgt gaggaacaca
    20761 tcgtgcgcgg ggaggtcgcg caggccggcg gtgagccaga ccgtggtcgt gtacgcgtcc
    20821 gcctgccggc cgccgccgtc ggcgccgtcc tgcgtctgcg cagccgcagc ggccagggcg
    20881 gcgatcgcct ctccgaggcc gcggctgagc gcgggcgcct cctcggccgc gcgctcgcag
    20941 gcgtccgcgg ccgccgtcag cgtacggcct tcgtcgggag ggagcctgcg cgccacggcg
    21001 cgcaggcgct gcggcagcgg ccgctgccgg tgacggccgc gcacgtcgcc gaggagctgg
    21061 tccagcgaga gcgcgtccac ccggtcgctg aacgcctccg tggcctcgtg gtacagcagg
    21121 tgcgcctcgt cgatcacgag gtcctcgaac gcggggagca tgtcgcggcc ggagagcagc
    21181 agcgcgtggt tgacgcacac caggtgggcg gcgtcggcgc gagcccgcgc caggcgccag
    21241 tggcagccgc ggcgtgcggg gcagaagcgc cccaggcagg tcgcgcgcga ggagcggagc
    21301 tctcgcgcca ggtcggagag ggcggggagg acctgtgtgg ccctgaaggg cagggcggag
    21361 aggtcgacgt cgccgcgccg ggcgcgtccg gcgaggtacg cgagcgccag tgtcctgtcc
    21421 cggtcgggga gcgcctcgcc ctcggcggcg accgcctcgt cgaggcgccg ccggcagagg
    21481 tagttctggc ggcccatgag cagggcccat cgccagccgg gggggagggc cgcggccacg
    21541 agcggcagct ccgtgcgcgc cagctgccgc tgaagcgcct tggtcttggt gctgacgagg
    21601 acgcgccgtc cggtggcggc gccggcgaag gccgccggca gcaggtaccc gaggctcttg
    21661 cccatgccgg tgccggcctc gaacagcccg atgccgccgg cgccgaagac accggcgacg
    21721 gcctccccgt actccacctg gccggcgcgc cggcagaacc cgggaaggcg cccggccagg
    21781 ccgggcacgg cgccgccgcc ggcgtccagc tccgcgcgcc agccgtccgg atcgacgggc
    21841 agcacggcca gcgggcccgg cccttcgggc ggctcgtccg ccaccagcgg cggaggcgcc
    21901 tcgtcgggcg ccgccgcgca ggcgtccagc acggggaggg gcgcccaggc gacgctctcg
    21961 agcaggcgcc gctccacgtc ggcgagcccg gcggcgcgcc gcgccaggag ggcgagcacg
    22021 gcggcggtgg cctgggcgtc cggcagcgcc ctgtgggcgc gccactcgag tccgagctcc
    22081 tcgaccagga tgggcagggc gtggcggtcg agttcggggt agagcaggag cgccgcgtcg
    22141 agcgtgtcga accagtcgcc ggtgagcggc gcggcgccgg cgcagcgcgc cgccgcggcg
    22201 aggtgctcac ggtcgaacgc cgcgttgtgg ccgacgagcg tcgcgtcgcc ggcgaacgcg
    22261 cgcagatccg cgagcgcgtc ggccgcgcgc ggcgcgccgg ccacatcggc gtcggagatg
    22321 ccggtgagcc gggtgatgaa cagggggatc gggatgccgg ggtctacgag cgtggtgaag
    22381 gtcgccgaca cgcgaagatc ctgatccagc cgcacggcgc cgatgtcgat gatccggtcc
    22441 gcccgcgggt ccaggccggt ggtctcggtg tcgatgacga cgaagtgggg acgcgggcgc
    22501 ggcatggccc cgaggatagc agagcaggag gccggatccg gccggccgcc ggccgggccg
    22561 ggagacgctc gcccggcgtc aggcggggag gcgctcgatg gccccggcgg ccatggtggc
    22621 gacgagcgcg agcagctcgg cctggccgtc gccggcgtcg ctgccgaggg tcgtggtgag
    22681 ggtgacctcg gtctctccgc acggtgcggc ggcgacgatg acgcgcgcgg acccggtctg
    22741 tgccagcgcc tggtccgcgg gacgcaggcc ggaggctccg gcgctcgcgc cggcgtggct
    22801 ggcgacgcag gagatggcgc cgagcgcccg cgccgagacc tcggccgcgg cctgcagcac
    22861 cccggcgggg ccggcctgct cgtgcagcgt ccggtagccg gcgacgatgg tctcgatggc
    22921 gtgccgcaac gtcagggcgt cctggtcgct gcgcgtgagc tgcgcgaaca cggtggcgtt
    22981 gtgcagcgcc accgccgcct gggcggccac ggcattggcg agacgcatct cctggcggct
    23041 gaacttgcgc tcgcgctggt ggtcgagcac ctcgatcagg ccgatgctct cgccctggtg
    23101 caccatcggc aggatgagca ggcacttgtc gccgtcgcgg cgcatgatgg cgacctccgc
    23161 cgggtcggcg tgcggatcgc cgacgttgac ggtgatgctc tcgtggtgct cgatcgcccg
    23221 tcgcgccatc gggaactggc cgagtgtgta ccgcatggtg tcctgccggc ggttgccccg
    23281 gtcgtcgtac tcggcgcgca ccgagaccgt gcgcgtggcg gggtcgtagt ccgagatgac
    23341 gcacaggtgc gcgtcgagca gcgtggtcat ctgcttcgcg atctcgcgca gcagggcccc
    23401 tgcgtcgagc tggctggaga cggcgacggc cgtcgcgacg agggtctcca gctctctctc
    23461 gcgcgcgagg cgcgcggcga ggtggttgcg cccgccgatg aggtgggcca gctcgtcgat
    23521 gtcctcttgc ttgatgcgcc ggtggccacc ggcggtgcgc gtgcagggga ggaacccgga
    23581 cgcggtccag cggcggatgg tgtgcgggga gacaccgagc cggcgcgccg cgtattgcac
    23641 gctcagatac atgcgcagac cgtagcttcg ccaccgtcgg gcgtcaagcc tgcgaacttt
    23701 tgccgcttgg ggacggaaag gttcttcagt cagtctctgg tcacgaaatg ccagcacaac
    23761 accgagttcg gcagtcactc ccgcggatga gagcgtgcag gttttgccgt cccttctccg
    23821 gcagcggcgg gaggaaccgg acgctcagcc ggcggcgacg acggcggcga ccccggccac
    23881 gaagtcgagc agctccgcct ggccctcgcc gggggcgtgc gggagcgtga cggcgagcgt
    23941 gaggtcggtg cggccggagg gatcgcgggc gacgaccagg ctggcgttgg cgccgcgcgc
    24001 gacgtccgcc ctggaggggg cggcgacccg gccggcgccg aaggcgccgg cgctctctcc
    24061 gcccaagctg gccacgcacg ag
//
LOCUS       DASUMX010000077         6374 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_597547, whole genome shotgun sequence.
ACCESSION   DASUMX010000077 DASUMX010000000
VERSION     DASUMX010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 6374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6374
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_597547"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="VFZ86_03615"
     CDS             <1..200
                     /locus_tag="VFZ86_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HEX5641410.1"
                     /translation="PTGGSLSDVRRKDVVIASKDWVAVDAYATRLFGKTPRFVPYIAA
                     AAKMGLGKSDLSGLVIRKYEV"
     gene            208..1809
                     /locus_tag="VFZ86_03620"
     CDS             208..1809
                     /locus_tag="VFZ86_03620"
                     /inference="COORDINATES: protein motif:HMM:NF024924.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX5641411.1"
                     /translation="MARAARRRTGGWKAARWARRAVQILSFAAFVYLLFAALQRLEPQ
                     PLTSIFFRFDPLAALATMLAARDWLAPFALAFITLGVTLVLGRVWCGWICPLGTLLGW
                     LRFRSARRLAGRVPTALRRVKYVLLGAIVVLAALANLTLLVLDPLSLLTRTLTTSAIP
                     GFVFVADTLARAGMSWGPTVGAVTWVEDAFRGTVLPTIQPRYEQAVALFLLLLTVVLL
                     NLLADRFWCRYLCPLGALLGLLAKLQLVRPVATEGCGACGACASACRVDAIATTRAGE
                     PAAKPIAVVSSECTMCLDCLVACPASGGMTLGAARPGPWVEYDPGRRSALIAVGAGVT
                     AAVLFSTGVARAVKHPGLIRPPGAQDEGRFLSHCLRCGECMKVCPTAGLQPALGEAGL
                     EGLWTPVLRSRLGYCDYACHACGQVCPSGAIPPLALPEKRRQVIGVAVIDKDRCLPWA
                     SNTPCVVCEEMCPVPEKAIELGGRRLITRANGSQDYLSRPRVVAERCIGCGICEYKCP
                     LEGRSAIVVVPASPTLATGSSAPLG"
     gene            1853..2575
                     /locus_tag="VFZ86_03625"
     CDS             1853..2575
                     /locus_tag="VFZ86_03625"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX5641412.1"
                     /translation="MSTDDRTLGDLRAAALFTRLDEGQLQRLAGVAQPRRLAADETLF
                     LQGDAATAFYLLAEGRLKVFKLLRDGRTATVRHVDPGQTFAEAALFHEVYPSNTETLT
                     ECLVYRFDKEQTLALLLAEPQLAVNLLAAMAHLLGLLNRRVEELLLPVPARLARYLLA
                     RADEQLEPARAGGDEGPRDVPRVIRLPVSKRELAARLGTVPETLSRTFDRFKRAQVIR
                     MSGDHKLIEILSFDQLHRLAQE"
     gene            complement(2588..3472)
                     /locus_tag="VFZ86_03630"
     CDS             complement(2588..3472)
                     /locus_tag="VFZ86_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014586922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX5641413.1"
                     /translation="MSAAEKRAQATPQAPRLATYAKNTWCPGCGNFAVLSAIRPVFQQ
                     LIASGTRPEDIVLVSDIGCNSKIMDYIGVNSFDSLHGRSIPTAVGVKLANPDLTVVVH
                     AGDGATFAEGLEHTLFAAKRNVDLTLIVHDNGVYGLTIGQAGPETPAGYRGRSTPYGH
                     VEDPFNPLELLYAAGATYLARGYSHGKELLKRLYAEAIAHRGFSVVDTLQVCVTFRDM
                     YASYNERVYELEGHDPRDEEQARAKILEWDYRSEGPVALGTFSVRDRAVFGDQFLEYG
                     TRSLDTDAALEETFADLT"
     gene            complement(3469..5256)
                     /locus_tag="VFZ86_03635"
     CDS             complement(3469..5256)
                     /locus_tag="VFZ86_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HEX5641414.1"
                     /translation="MDSFSVLIGGKAGDGITQAGSMIAHLLNELGYRLRTYVDSPSLI
                     RGGHNFVIVRAHRGRIAAHRDHINVMIALTPDTLERHEWRLKPRPITVFDNQTIDPTS
                     LPSVAHPVRTGIPVKTILEQEQAPSVMRNSAILGAFCKAMAIPWETLEDVIRRYQPKQ
                     TELNLRVARRGFDLATEKLLIHLPGGGPRPVMTGLEATGLGLIRGGLRAYAGYPMTPA
                     SGLLHFLAREAPRFELKVVQPEGEIAAVMMALGHAYAGERAAVGTSGGGFALMVEGLS
                     LAGQAELPIVFVLGQRPGPATGLPTYTAQGDLHFAMSAGHGEFPRLIVAPADVEQAYA
                     WSTFAMDAAWKYQMPAIVLGDRTLNDDAQSFDRDAVPDLPRLEPRLWDGVPQRSADDV
                     PVAAGAGTGAARERGGVAAGAPPPGDEGLAYKRYLFAEDGISPLAFPGREGAVVKVNS
                     YTHDEFGITTEDPELTVRMQDKWNAKGFALAEELDGIQQVYVYGDPDAKETLVTWGSP
                     TGAAREVGELAGIRVVQPVVLWPFPDRQMAEALEGTETLAVAEGNSRGQLARLLEQRG
                     FHIDGRLLRYDGRPWGVGELRRQAEEVLA"
     gene            5413..5853
                     /locus_tag="VFZ86_03640"
     CDS             5413..5853
                     /locus_tag="VFZ86_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641415.1"
                     /translation="MASTSRLRTIGKVGGAAAVTAPYVRRLAGDEELRDDVADFIRSA
                     NNIMTRVRSDKRLRGDVRRMVESAQSGAGHLSGDFRPRRTLRRLFVGAGLIITAVGVG
                     IAVAWPRTRRQITQAVEQGTSRANAVVHDVRERVSGESSETRAA"
     gene            6033..6269
                     /locus_tag="VFZ86_03645"
     CDS             6033..6269
                     /locus_tag="VFZ86_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014687164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6458 family protein"
                     /protein_id="HEX5641416.1"
                     /translation="MGIGTSIFLIALGAILRYAVTATVSGISIQTIGLILIIVGVVGL
                     VLSLIFWSALGSWYPGRSSTVVRRDECVDGNCLT"
BASE COUNT          820 a         2345 c         2239 g          970 t
ORIGIN      
        1 gccccaccgg cggcagcctg agtgacgtga ggcgtaagga cgtcgtgatc gcgagcaagg
       61 actgggtcgc ggtcgacgcc tacgcgacgc ggctgttcgg caagacgccg cgcttcgtgc
      121 cgtacatcgc cgccgccgcc aagatgggcc tcggcaagtc cgacctctcg ggcctcgtca
      181 tccgcaagta cgaggtctga cgaggccgtg gccagagcag cgcgccggag gacgggcggc
      241 tggaaggccg cccgctgggc ccggcgcgcc gtgcagatcc tctcgttcgc cgccttcgtg
      301 tacctgctgt tcgcggccct gcagcggctg gagccgcagc cgctcacgag catcttcttc
      361 cgcttcgacc cgctggcggc gctggcgacg atgctcgccg cgcgcgactg gctcgccccc
      421 ttcgccctcg ccttcatcac cctcggggtg acactggtgc tcggccgcgt gtggtgcggc
      481 tggatctgcc cgctgggcac gctgctcggc tggctgcggt tccgctccgc gcggcggctc
      541 gccgggcgcg tgcccactgc cctccgccgc gtcaagtacg tcctcctcgg ggccatcgtc
      601 gtcctcgcgg cgctggccaa cctgaccctg ctggtgctcg atcccctgtc gctgctcacg
      661 cggacgctga cgacctccgc catcccgggc ttcgtcttcg tcgccgacac gctggcgcgc
      721 gccggcatga gctgggggcc gacggtgggc gccgtcacct gggtcgagga cgccttccgg
      781 ggaacggtcc tgcccaccat ccagccgcgc tacgagcagg ccgtcgctct cttcctgctc
      841 ctcctcaccg tcgtcctcct caacctgctg gcggaccgct tctggtgccg gtacctgtgc
      901 cccctcggcg cgctcctcgg actgctcgcc aagctgcagc tggtgcgccc ggtggccacg
      961 gagggctgcg gcgcctgcgg cgcctgcgcc tccgcctgcc gcgtcgacgc catcgcgacc
     1021 acccgcgccg gcgagccggc cgccaagccc atcgcggtcg tgagctccga gtgcaccatg
     1081 tgcctggact gcctcgtggc gtgccctgcc tccggaggga tgacgctcgg cgccgcgcgg
     1141 ccgggcccct gggtggagta cgaccccggc cgccgctcgg ccctgatcgc cgtgggcgcc
     1201 ggcgtgacgg cggccgtgct cttcagcacg ggggtggcgc gcgccgtcaa gcacccgggc
     1261 ctgatccggc cgccgggcgc gcaggacgaa ggccggttcc tctcgcactg cctgcgctgc
     1321 ggcgagtgca tgaaggtgtg tccgacggcc gggctccagc cggcgctcgg ggaggccggc
     1381 ctcgagggcc tgtggacgcc cgtcctcagg tcgcgcctcg ggtactgcga ctacgcctgc
     1441 cacgcctgcg gccaggtctg cccctccggc gccatcccac ccctcgcgct ccccgagaag
     1501 cgccggcagg tcatcggcgt cgcggtgatc gacaaggacc gctgtctgcc ctgggcctcg
     1561 aacacgccct gcgtggtctg cgaggagatg tgcccggtcc cggagaaggc gatcgagctc
     1621 ggcgggcgcc ggctcatcac ccgcgccaac ggcagccagg actacctctc gcgcccccgg
     1681 gtggtcgccg agcgctgcat cggctgcggc atctgcgagt acaagtgccc cctggagggc
     1741 cgttccgcga tcgtcgtcgt gcccgccagc ccgaccctcg cgacgggctc ctcggcgcca
     1801 ctgggctgac caggacatcc tcgccacggg aacgcgcggc tatacttccg ccatgagcac
     1861 cgacgaccgc accctcggcg acctgcgcgc cgccgcgctc ttcacccgcc tggacgaagg
     1921 ccagctgcag aggctggccg gcgtggcgca gccgcgccgg ctcgccgcgg acgagacgct
     1981 cttcctccag ggcgacgcgg ccaccgcctt ctacctgctc gccgagggca ggctcaaggt
     2041 cttcaagctg ctcagggacg ggcgcacggc gacggtgcgc cacgtcgatc ccggccagac
     2101 cttcgccgag gcggcgctct tccacgaggt ctatccgtcg aacacggaga cgctgacgga
     2161 gtgcttggtc taccggttcg acaaggagca gacgctggcg ctcctgctcg ccgagccgca
     2221 gctcgccgtg aacctgctcg cggcgatggc gcacctcctc ggtctgctca accggcgcgt
     2281 ggaggagctg ctgctgcccg tgcccgcgcg cctcgccagg tacctgctgg cgcgcgccga
     2341 cgagcagctc gagccggcgc gcgccggcgg cgacgagggc cctcgcgacg tgccgcgcgt
     2401 catccggttg ccggtcagca agcgcgagct ggcggcgcgt ctgggcaccg tgccggagac
     2461 gctcagccgc acgttcgacc gcttcaagcg cgcgcaggtc atccgcatga gcggcgacca
     2521 caagctcatc gagatcctca gcttcgacca gctgcaccgt ctcgcccagg agtgagggcg
     2581 aggacggcta cgtgaggtcc gcgaacgtct cctccagcgc cgcgtccgtg tccagcgacc
     2641 gcgtgccgta ctccaggaac tgatccccga agaccgcccg gtcccgcacg ctgaacgtgc
     2701 ccagcgcgac cggcccctcc gagcggtagt cccactccag gatcttggcc cgcgcctgct
     2761 cctcgtcgcg cgggtcgtgg ccctcgagct cgtacacgcg ctcgttgtag gaggcgtaca
     2821 tgtcgcggaa cgtgacgcag acctgcagcg tgtccacgac gctgaagccg cggtgcgcga
     2881 tggcctccgc gtacaagcgc ttgagcagtt ccttgccgtg cgagtaccca cgcgccaggt
     2941 aggtcgcccc agctgcgtac agcagctcga gggggttgaa cgggtcctcg acgtggccgt
     3001 agggagtcga ccgtccccgg tacccggccg gggtctcggg accggcctgg ccgatcgtga
     3061 gcccgtacac gccgttgtcg tggacgatga gcgtgaggtc gacgttgcgc ttggcggcga
     3121 acagcgtgtg ctcgagcccc tccgcgaacg tcgcgccgtc gccggcgtgc acgacgacgg
     3181 tgaggtccgg gttcgcgagc ttgacgccga ccgcggtcgg gatcgagcgg ccgtgcaggg
     3241 agtcgaagct gttcacgccg atgtagtcca tgatcttgct gttgcagccg atgtcgctga
     3301 ccagcacgat gtcctccggc cgcgtgcccg aggcgatgag ctgctggaac acgggcctga
     3361 tcgcgctgag caccgcgaag ttgccgcagc cggggcacca ggtgttcttc gcgtaggtcg
     3421 cgaggcgagg ggcctgcggc gtggcctgcg cccgcttctc cgcggcgctc atgccagcac
     3481 ctcctcggcc tggcgccgga gctcgccgac gccccacggc cgcccgtcgt agcgcagcag
     3541 gcggccgtcg atgtggaacc cgcgctgctc gagcagccgg gcgagctggc cgcgggagtt
     3601 gccctcggcc acggccagcg tctccgtgcc ctcgagagcc tccgccatct gcctgtccgg
     3661 gaacggccag agcacgaccg gctggacgac ccggatgccc gcgagctcgc cgacctcgcg
     3721 ggccgcgccc gtgggcgagc cccaggtcac cagcgtctcc ttcgcgtccg gatcgccgta
     3781 cacgtacacc tgctggatgc cgtccagctc ctccgccagc gcgaagccct tggcgttcca
     3841 cttgtcctgc atgcgcaccg tgagctcggg gtcctccgtc gtgatgccga actcgtcgtg
     3901 cgtgtagctg ttgactttga ccacggcgcc ctcgcgaccg gggaaggcga gcggagagat
     3961 accgtcctcg gcgaacaggt agcgcttgta ggcgaggcct tcgtccccgg gcggcggggc
     4021 gccggcggcc acgccgcctc tctcgcgcgc cgcacccgtg ccggcgccgg cggcgacggg
     4081 gacgtcgtcg gcgctccgtt gcgggacgcc gtcccagagc cgcggctcca gccgcggcag
     4141 gtcggggacg gcgtccctgt cgaagctctg cgcatcgtcg ttcagcgtcc ggtcccccag
     4201 cacgatggcc ggcatctggt acttccaggc cgcgtccatc gcgaacgtgc tccaggcgta
     4261 ggcctgctcc acgtcggcgg gagcgacgat gaggcgaggg aactcgccgt gcccggcgct
     4321 catggcgaag tgcaggtcgc cctgggccgt gtacgtcggc aggccggtcg ccgggccggg
     4381 gcgctgcccg agcacgaaga cgatgggcag ctcggcctga ccggccaggc tgaggccctc
     4441 gaccatgagg gcgaagccgc cgccggaggt gcccacggcg gcccgttcgc ccgcgtacgc
     4501 gtgccccagc gccatcatga cggcggcgat ctcgccctcc ggctgcacca ccttgagctc
     4561 gaagcgcggc gcctcccgcg ccaggaagtg cagcaggccg ctcgccggcg tcatcgggta
     4621 gccggcgtac gccctgaggc cgccgcggat gaggcccagg ccggtcgcct ccaggccggt
     4681 catcaccggt cgcggcccgc cgccgggcag gtggatgagc agcttctcgg tggcaaggtc
     4741 gaagccccgg cgcgcgacgc gcaggttgag ctccgtctgt ttgggctggt agcgccggat
     4801 gacgtcctcc agcgtctccc aggggatcgc catcgccttg cagaaggcgc cgaggatggc
     4861 gctgttgcgc atcacgctcg gcgcctgctc ctgctcgagg atcgtcttca ccgggatccc
     4921 cgtgcgcacg gggtgcgcca cgctgggcag gctcgtcggg tcgatcgtct ggttgtcgaa
     4981 caccgtgatc ggccgcggct tgaggcgcca ttcgtgtcgt tccagcgtgt ccggggtgag
     5041 cgcgatcatg acgttgatgt ggtcacggtg cgccgcgatg cggccgcgat gcgcgcgcac
     5101 gatgacgaag ttgtgaccgc cacggatcag cgacggcgag tccacgtacg tgcggagccg
     5161 gtagccgagc tcgttgagca gatgcgcgat catggagccg gcctgggtga tcccgtcgcc
     5221 ggccttgccg ccgatgagca cggagaacga atccacgcgg gcctcctcgg gtgccgattg
     5281 tcagtgtcgt tcttacctgc gccggagccg cgcgtccacc cctcgaggcg acgcattaca
     5341 gtcaatgccg cggccgagcg ggaacatccc gagtatctgc aacccgacct cacgagagga
     5401 cggtggctca ccatggcttc cacgagcaga ctacggacga tcggcaaggt aggcggggcg
     5461 gcggcggtca cggctcccta cgtgcggcgc ctcgccggcg acgaggagtt gcgcgacgac
     5521 gtcgccgact tcatccgctc ggccaacaac atcatgaccc gcgtgcgctc ggacaagcgg
     5581 ctccgcggcg acgtacgccg catggtggag tccgcgcaga gcggcgccgg ccacctcagc
     5641 ggcgacttcc gcccgcggcg cacgctgcgc cggttgttcg tcggcgccgg gctgatcatc
     5701 acggccgtcg gcgtcggcat cgccgtggcg tggccgcgga cgcggcgcca gatcacgcag
     5761 gcggtcgagc agggcacgtc gcgagcgaac gcggtggtgc acgacgtccg cgagcgggtg
     5821 tccggcgaga gcagcgagac gcgggctgcc tgacgcggac gggtcatgtg gacagtgacg
     5881 gtgggggacg gggcgctccg cgcgaggaga tccccgtccc ccacggccct gccgccttcc
     5941 cagcgtcacc gggagtgcgt tttgccgccg gggcgccggg aagagcacat acgtcacacg
     6001 tgctcatcca cgccgatctc ggaggtcctt caatgggcat cggcaccagc atcttcctga
     6061 tcgccctcgg cgcgatactg cgctatgccg tcaccgcgac cgtatcgggc atcagcatcc
     6121 agaccatcgg cctgatcctc atcatcgtgg gcgtcgtcgg cctcgtcctc tcgctgatct
     6181 tctggtcggc gttgggctcc tggtaccccg gccggtcgtc gacggtggtc cgccgtgacg
     6241 agtgcgtcga cggcaactgc ctgacctgag gccgggccac gcaaggcggg gggccggggc
     6301 tgtcgcgacc gccccggccc cgctttgacg acccggcgca tggtcccggg ctgcactggt
     6361 cagccgttga cgat
//
LOCUS       DASUMX010000078        25248 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1172268, whole genome shotgun sequence.
ACCESSION   DASUMX010000078 DASUMX010000000
VERSION     DASUMX010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 25248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25248
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1172268"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..961)
                     /locus_tag="VFZ86_03650"
     CDS             complement(<1..961)
                     /locus_tag="VFZ86_03650"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX5641417.1"
                     /translation="MSSFDVAYVRSQFPALKRMVDGQPAAYLDGPGGTQTPQRVVDAV
                     VDYLVNHNCNIHGSFATSEETDAVVQSAHEAGADLLGCHWEEVSFGANMTTLGQLLSD
                     AVARDIRPGDEVLVTQLDHEANRGPWLRLAERGAVVREVPVDLETCTLDWDAFEKLVE
                     PGKTRVLAIGHASNAVGTVNDVARAAALAREAGALSVVDSVHYALHGPIDVRAIGCDF
                     LLCSAYKFFGPHVGLMYARRQTTEALEPVRLSTQEPDPPYVWETGTLNHEGMAGTTAA
                     IDFIADLGERHVAMVEDRLPAGLQGRRRAVVAGMLAGEAYEQPL"
     gene            1048..4290
                     /locus_tag="VFZ86_03655"
     CDS             1048..4290
                     /locus_tag="VFZ86_03655"
                     /inference="COORDINATES: protein motif:HMM:NF015588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HEX5641418.1"
                     /translation="MPTGGDNTRRVRARTHEPRLERALARRFPVICFAWDGTAVARRE
                     ADASAVRSRVERLTALGVDIAVISSAHVTEVDEQLRARPGVEGRLFLYLSRGSEVYVV
                     GPAGPRLLERRQSTPLEKELLVGIAEALRDRLAAAGLHVELVRHHLNRRSVDLIPDWP
                     EPPGAEVEELQRQVEARLRDAGFAGGLKECMEIARQLGRDAGLPEPAVTSDVRHIDIG
                     LTGKGDAMHAVSRALVAGRGRQPQDLLVLGDTFGPVADGQGTDSAMLIPELRRAVYVS
                     VGAEAFGVPPQVLHAGGGPVSFLDILHDQLAMWEIVAQRSFPEPTGDPAWRFEVKGFD
                     PFREREVETWLTVANGETGTRGALEEGSAISTPATFVAGVYGDGTGDPPFRQPVPAPD
                     WTGLRLMVAGSAMTLSNGEIIDHERVLDLRHGVVYRTWRQRLRSGRTVRVRTARFASL
                     ADRQLMALRAEATPEDFSGAIVWQSAIGVTHAGGPTRETAFEILDDPPGMIARTRGRN
                     GGGHVLAVTTNPAPGSPVARTPQQARDVIGGRLEPGDPATVDRLASIVSARTKVPSAD
                     VARRALERARRTGYDELLRRHQAAWDAAWDAADLRVDGDAEDQHALRFAIYHMISTAH
                     PGKDTVSIGARGLGGMSYFLHVFWDTEIFVLPFFIYTQPETALTMLKYRYRNLAGARE
                     KAARMGFRGALYPWESADKGVETTPPYGYGPSGEQVPILSGLMEHHISADVAWAVWEY
                     WKATGDDAFMASMGVEMLLETARFWVSRAASDRQGRYHVRVVVGPDEYHEGVDDNAYT
                     NVLARWNIKRALEALAWLEGVDRDRAVELKKRLGTTGSELSTWRRVLDGYVTGFDPET
                     LLYEQFAGFYDMADVPIEKLRPRPMAADLLLGRDVTLVSKVVKQADVVMLCHVLADEI
                     DEATTRANYDYYEPITCHGSSLSPGIHAAVAARLHDIPEATEYFKMAAAIDLADNMGN
                     AAAGLHMATMGGLWQAAVMGFCGIMRRGEALRLDPQLPEAWKRVRMPLRFRGARLSLD
                     LRARKDGVDVGITVENAPVTLQLDGVEREFPPGRHRLRRRGGGPWQVEAG"
     gene            4287..5090
                     /locus_tag="VFZ86_03660"
     CDS             4287..5090
                     /locus_tag="VFZ86_03660"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5641419.1"
                     /translation="MTLLVAVADAQADRELIASARALAAAARWEIRAVHVREPDVPEP
                     GSADLEGLELSAIDGDPVESLTRLVGGGSEVDAFAFGLSCSCAPGPAAGIGHVAEALL
                     NSHVAPVLLVRPGMRPVAGLKRLYVPLEGSPSSSAAMRAADDALCARGREIVMLHVVT
                     GGETPAETGSLPAPRMTDQEHYEWSAWQDEFTMRFSQCPEGGRHRVAVRVGVPAEIIA
                     EEAGKNGAELIVLSWNGSFESGHGAVIKELLVTAPCPLLLVPSGVPEAV"
     gene            5292..5414
                     /locus_tag="VFZ86_03665"
     CDS             5292..5414
                     /locus_tag="VFZ86_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641420.1"
                     /translation="MIDVLLMALAIAFFAAADLFVRGCTRILERGSDEHREDGQ"
     gene            5411..5503
                     /locus_tag="VFZ86_03670"
     CDS             5411..5503
                     /locus_tag="VFZ86_03670"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0043462 - regulation of ATP-dependent
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit F"
                     /protein_id="HEX5641421.1"
                     /translation="MTAADVVFFVIIAALLAYLTWALIAPEDLQ"
     gene            5526..7202
                     /gene="kdpA"
                     /locus_tag="VFZ86_03675"
     CDS             5526..7202
                     /gene="kdpA"
                     /locus_tag="VFZ86_03675"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017537411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HEX5641422.1"
                     /translation="MTTSAWLQAAALVGLLLLGTRLLGPYLAKVYAGAGAPGDRAFLP
                     LERLVYRAARIDPENEQPWTVYARSVIAFSLVSVLGLYALQRLQGGLPLNPTSVESTP
                     PFLAFHTAASFVSNTDFQNYAGEATMSHLVQMAGLTVQNFVSAAVGMAVAVALIRGFT
                     RERAGTIGNFWVDLTRGVTRVLLPLALVLALALVALGAVQTLQGPVDVATVEGATQTI
                     YRGPIASQESIKELGTNGGGVVNANSAHPFENPTGLTNLLEIFALLLIPFALTFAFGR
                     LVKDQRQGWAIFVAMFVLWAGSVATATSFEVQGNPRVEVGAANMEGKEVRFGAATSAL
                     FAASTTGTSTGAVISSHDSFTPGGGAVPLVNILLGEVSPGGVGAGLYGMLVFVLLAVF
                     ISGLMVGRTPEYLGKKVQAAEMKLVVLYILVVPVAVLVFSGVSVVLGPARESILNPGP
                     HGLTEVVYAFASQANNNGSAFGGLTGNTDWYNTTGALAMLAGRFFLIVPALAIAGSLA
                     RKKTVPPSAGTLPTHNPLFVVMLLAVIVIVVGLTYFPVLALGPIVEQLAL"
     gene            7217..9208
                     /gene="kdpB"
                     /locus_tag="VFZ86_03680"
     CDS             7217..9208
                     /gene="kdpB"
                     /locus_tag="VFZ86_03680"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HEX5641423.1"
                     /translation="MVDRRLMRRAAQDAAIKLDPRRMARNPVMFVVEVTSVLVTALAV
                     LEPSVFAWSIAAWLWFTVLFANFAEAMAEGRGKAQAESLRRTRSETTAHRRGEDGGLE
                     DVPSSGLALGDVVVVAAGELIPADGEVIEGIATVDESAITGESAPVIRESGGDRSAVT
                     GGTRVLSDQIVVRVTQEPGASFLDRMIALVEGSSRQKTPNEIALDILLAGLTVIFLIA
                     VATLQPFAVYSGARQDVIVLAALLVCLIPTTIGALLSAIGIAGMDRLVQHNVLARSGR
                     AVEAAGDCSTLLLDKTGTITLGNRQAAEFLPLPGVTELELADAAQLSSLADETPEGRS
                     IVVLAKERFNLRERELSGATLIPFTAQTRISGVDIDGRSIRKGAAEAVRRLLADGGQE
                     PPRELDELVDGVAAAGGTPLVVTENGCALGVIRLKDVVKDGMRERFDELRRMGIRTVM
                     ITGDNPRTAQAIAEEAGVDDFLAEATPEDKMALIRREQAGGALVAMTGDGTNDAPALA
                     QADVGVAMNTGTQAAREAGNMVDLDSNPTKLIEIVEIGKQLLITRGSLTTFSIANDVA
                     KYFAIIPAMFAGAFPVLDALNVMDLSTPQSAILSAVIFNALVIVALIPLALRGVRFRA
                     VSAASALQRNLLVYGLGGIVAPFVGIKVIDVVLSAFGVV"
     gene            9208..9780
                     /gene="kdpC"
                     /locus_tag="VFZ86_03685"
     CDS             9208..9780
                     /gene="kdpC"
                     /locus_tag="VFZ86_03685"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HEX5641424.1"
                     /translation="MRRQLPTAIVVFAVFSVLVGVVYPLVVTGIAQVAFGSKADGSLV
                     VRDGAPVGSSLIGQAFEGPGYFHPRPSAAGDGYDAMASAPSNLGPSDPGLLDAVAQRK
                     EEYREENGLGAGERVPVEAITSSGSGLDPHISPADARLQARRVAEARGVSLTRVLGLV
                     DEATEGRPLGIFGDPGVNVLELNLALDERR"
     gene            9792..12278
                     /locus_tag="VFZ86_03690"
     CDS             9792..12278
                     /locus_tag="VFZ86_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020520830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HEX5641425.1"
                     /translation="MSRGTLRIYLGAAPGVGKTYAMLNEGRRRQTRGEDVVVGFVESH
                     GRQRTSEQVGDLEVVPRAPVAYREAVFEEMDIDAVLARRPQLALVDELAHTNVPGSRN
                     QKRWQDVEELLDAGISVISTLNIQHLESLNDVVEQITGVVQRETIPDEKVRRADQLQL
                     VDLAVETVRARLARGDIYPAERIDAALGNYFRAGNLSALRELALLWIADRVEEGLEEY
                     RARHGISETWETRERVMVALSGSADGDRLVRRAARMAQRLNAELIAVHVRPQTGLATQ
                     SAGRLEQQGRLVERLGGAYREVSGADVGEALVDTARALNATQVVVGASERSPLSLLLH
                     GSVVRTVIRLSGVGLDVHVIGRQADAEPEAARSQRLRAPRALPRRRVALGFVLAVVGA
                     PLLAWALTHARDQVSPSGALLLFLLLVVAVSALGGLWPALLAALGGFLLVNWYLTPPL
                     YTWTIGDPEDVLALLVFLAVAVAMSFFVALAARRAAQATRARAKAETLMRLAGSSSME
                     AVLEGLRRSFGFESAALLHRDDHEWTVEAASGSAPPSLDTAELVVEVDAGHVLVASSP
                     APRRTEDVRMLEAFARELAASVEHEELEAEVASAEGLAAVNELRASILSSVSHDLRTP
                     LSAIKASATSLLQDDVDWTPEAERELLTTIDEETDRLNRLVGSLLDMSRLQAGALEVQ
                     EEVIILDEVLAEAMASVGAGSTEVVLDLPEDLPPVVGDEALLERALANIVANAVTYSR
                     GEPVLVSAGVVPDGVDVRVADRGPGVPLQERETIFLPFRRVGDTRRHEGAGLGLAIAK
                     GFVEAMGGTIEADDTPGGGLTIVVHLKVMK"
     gene            12275..12958
                     /locus_tag="VFZ86_03695"
     CDS             12275..12958
                     /locus_tag="VFZ86_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018385851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5641426.1"
                     /translation="MTRVLVVDDEPQILRALRINLRARGYEVDVAADGESALRLAARD
                     HPDVVVLDLGLPGIDGIEVIEGLRGWTSVPIIVLSVRDREADKVAALDAGADDYVTKP
                     FGMDELLARMRAALRRVTPADEPVLIETPDFTIDLAARRVTRAGEEVRLTATEWHILE
                     VLVRNAGRLVTQRQLLQAVWGPKYETETHYLRVYVGQLRRKLEPVPSEPRYFLTAARM
                     GYRFEPEAP"
     gene            complement(12984..13805)
                     /locus_tag="VFZ86_03700"
     CDS             complement(12984..13805)
                     /locus_tag="VFZ86_03700"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HEX5641427.1"
                     /translation="MRPVLFQIGDYRFYSFGLMAALALIVPGMTIVRPLVRRRGVPGE
                     FAYELIIAAGVGGFIFARIYYLIENYALIRDDFWSAAFGGIGFTWYGGLIGGFLGVVG
                     WTLIRRYPLDVIANAMAPATAFGYLIGRIGCQLAGDGDYGKPSDLPWAMGYPNGTVPT
                     PPGVEVHPTPVYEVIAMAFVVWILWRLATRYDKSGWWTFGWFLVLSGIERFLVEFVRI
                     NTVGFGGLTQPQWVSIVSILIGVTLIAVFRGRPAVLVGAAARPSRAQKRRAARAA"
     gene            complement(13810..14832)
                     /locus_tag="VFZ86_03705"
     CDS             complement(13810..14832)
                     /locus_tag="VFZ86_03705"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX5641428.1"
                     /translation="MTLVICPNLAIDRMLAGGPPQPGGMTRCRALRTQAGGKGANVLR
                     ATLALGGDGRLAGFAAGRGGRLIAELAREEGLELAVVATEGEARVSTVVLGDDGSVTR
                     LYEFGPEISSADERALLELAGGRDAGRGEWAVVTGAAPPGASPGFYAALVSTTRAAGY
                     RVLVDADGEQLAGAAAERPDLVKVNLAEACSAVGAPGARCDDEQDAAPEALVAEGLEL
                     CRRLIDAGAEGAVLTLGAAGAAAVVDGAEWRVATRQVRAVNPVGSGDCFAAGLTLALQ
                     RGEGTAAALRLAAAAGAANAVSPYNGDVDVALVEELAGEAFAGPPGRRASAGPTAGRR
                     PPHPLH"
     gene            complement(14867..16189)
                     /locus_tag="VFZ86_03710"
     CDS             complement(14867..16189)
                     /locus_tag="VFZ86_03710"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEX5641429.1"
                     /translation="MSTPSGHDKPYSLYRAGTTPDEDVAGAPSGDGGPPRDRGDRGRR
                     RGRRGGGFVLLLIVLGIIVAAAAGVAAAAGWGPFGGGGEAAAEQASPAATGAGAADLT
                     SAPSAEPSPSASPSPTPAAAPTFSVTAGGDVIGGFGVGGVVESMGSGLFESIAPTFEK
                     SDFGFVNLESPLTTGGEAQGWKDVVIKGNPDLAPAMAESGINVVTMANNHAGDMGDSG
                     LLDSIEYCEKYDIAVVGAGEDLDAALKGTVLETEDGVRVAFLGFTDVLPVGYPATSSS
                     PGVAPGRADIGAVTKAIRAAAKKADFVIVGWHWNFEYKRAPSALEESEGKAAVDAGAD
                     IVFAHHPHLLNGVEAYHGGLIFYSLGNLVFSGFSGETAETVLVRAKVSEERIDAQLIP
                     VVGGGSGVPGVVKGEQADSILQRFQGFSSALDTNVKIADGKGYVHLKR"
     gene            16353..17603
                     /locus_tag="VFZ86_03715"
     CDS             16353..17603
                     /locus_tag="VFZ86_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641430.1"
                     /translation="MRAAHRIASLVLATVAAATLWAAAAGCGIDLGASDDDARADLER
                     TLRLARHEFAAAADALAAGDRAAFLDWLPHDSGSPGSVAAREGLAELFDRLAPLPWRT
                     FSFEVTPLDPAEGVFRVRGIGQLGDAGPPDRIAVVRYFRLLAVADGAVVLADETPEDL
                     RRRYLMALHDPLVLQRPGLIVLGDAAARGRAAAVMAAAARARPQLAALGVGAGPSVVI
                     TVYGSAADVRDALALGASTARLVFFSHPSLRVAEDSWPTYDVGVMGPWLRDRGGSMDA
                     VLRHELAHAYTVSWFGDDEEPPALLVEGIAQAAEGTAATPALRQEVATGDQLWPLPES
                     FADTDVWDGGDAAAVRLGYEIGGALVAYVVSRWGPEQLRPFVQALAAAEPSEAGMDAA
                     LGGTLGVSWREFFAGWRDSVLTGD"
     gene            17645..18895
                     /locus_tag="VFZ86_03720"
     CDS             17645..18895
                     /locus_tag="VFZ86_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641431.1"
                     /translation="MTAPSRRARPALLVAGAGLAILLVALVAAGVGLSRREEPAQPRA
                     AAPRVTTRDVQAALDDATPALLQRDRAAWDAALPASGGDARHGVDVLYRRLAPLPWTD
                     LRLIAEAVRGRPGRFYVGAVGEVGDAGPADRIMARRVFDAGLRGGRVVLRDDVTPRDL
                     RGQGVMTFARPVAVRRHGLVVIADERERPAAEALAATGATARERLELLGISASKPIVV
                     YFYSSRRQLLRSLGEDPGEARIRFFSHAPKRLRDVPTWTRDIGVLGPALRGKASWTPR
                     MLAHEMTHAYTSRWFEGTRNAPTLLAEGLATAVEGGRSFQPLRDDLASPESAFPLEKA
                     LKAKSLWKGNRIAKVRLAYLEGASLVLYVRDRWGLRGLREFVAAVSDSDLTSQGLDEA
                     ARRSVGVGWKALRAGWAAYVETLP"
     gene            19026..20405
                     /locus_tag="VFZ86_03725"
     CDS             19026..20405
                     /locus_tag="VFZ86_03725"
                     /inference="COORDINATES: protein motif:HMM:NF015825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB
                     domain-containing protein"
                     /protein_id="HEX5641432.1"
                     /translation="MRSRVLILATLVGLAAVVLVIAVAVAGAGQSDPLPDISAPDLLG
                     KMAQAGDVTAVSGDVAWQNDLFGDLAQASGMAQLPAQSPLTSSGSGRIWVSDAGARVE
                     SQGGGGDQVVVVDKAARTAWVYDYAQNTVKKVVVTGTAPAETPSPAPAATALTPAMIT
                     LSLQQAARFATVEVAGQTKVAGRDAYQLRMTPVATDTALGYVEAAVDGETSLPLQLDV
                     YARGGTVPVLRFGFTSVSYDAIDPATFAFTPPEGAQVTTRTVDGDEMRREMQRAHEGD
                     AGTEPTAEQKTAAEKAVRGALLTREQVQELVPYQLAWARDYAARPFRWGYVLGPAGPL
                     TGSGAPLMELMGAATGMDIDALSPAGSHSVREGSAAGPSSALLYGDGFGAITLGQTRT
                     TAELEKQLAEARKTSQILGKATVGGAEALKIGTPLGGVIVWEKDGTTLVAAGMVPMSD
                     LEAFASSVR"
     gene            20449..21471
                     /locus_tag="VFZ86_03730"
     CDS             20449..21471
                     /locus_tag="VFZ86_03730"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5641433.1"
                     /translation="MSEPEGARPGQQSCDGRAPILTTHALTKQFGRLRAVDGLDMQVC
                     KGDLFGFLGPNGAGKTTVIRLVLGLIHPTSGHAEVCGHRVPGELQGALRHVGGFVDDP
                     TFYPLMSARRNLRLLGSMTGPVSEERIDEVLEIVALSDRAEDRVGGFSHGMKQRLGIA
                     QALLHSPELIVLDEPTSGLDPRGMKDVRELIRDLGAAGTTVFLSSHLLHEVEQVCTRA
                     VIINRGRVVVQGPVSELRPKNDAIKVLTGDQGRAAGVLRGQFGAGEVKEDEGFLVVRA
                     DEDAVPEMVRRLVEDGVEVRAVVPASEQGLEDFFLELTGTDAPGEPAGGKRRAGLARL
                     LPGGRR"
     gene            21468..22358
                     /locus_tag="VFZ86_03735"
     CDS             21468..22358
                     /locus_tag="VFZ86_03735"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX5641434.1"
                     /translation="MSAVPGPSGSAPPRAHGSTAAGMRGHAWGALRREWIKLLFQRRS
                     YLIWGGAFLIPFLISLALYLAKSDPHGGDGPLFFERITSNGMFVTLASLSALLPFLLP
                     MAAAMVAGYMVAGEAELGTLRIVLLRPTRRGAFILAKWTMAVVYLAVGFAVMVAGGLL
                     FGGVFFGLHPMITLSGSTVGVWQGLGLIVLSCLYGLAAMACIVSMALLFSTLTDSSLT
                     ALIATIVIYIVITVLIQFSYFDWLRPWVFPQYLTEFVNFWRDPIYWRPILKGLGVFAL
                     WSGGLTLAAYLVFRRKDVLS"
     gene            22373..22693
                     /locus_tag="VFZ86_03740"
     CDS             22373..22693
                     /locus_tag="VFZ86_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641435.1"
                     /translation="MPALVKASVALFLAAEVLIVAAIGADAIFMVIAWAVVAAATAGV
                     ALVVHASAARVLLAALLVAACVLFAVELGLFFLPAAAALLAAAVSDQQQHDHHHHGIL
                     HGRG"
     gene            22698..23852
                     /locus_tag="VFZ86_03745"
     CDS             22698..23852
                     /locus_tag="VFZ86_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEX5641436.1"
                     /translation="MAGTGAPATMRAMQLAAPAPVAEAPLRLVELPVPAPGPGEVLLR
                     VLACGVCRTDLHILEGELPPHRSPVVPGHQIVGEVVALGRGTELVAPGGEPGAPALAA
                     GDRVGVPWLHRTDGSCRFCARGRENLCEHALFTGWDVDGGFAEYTVAPAGFVYRLPAG
                     MTAMAAAPLLCAGIIGYRCLRLVGIVGDDGALPGGVAAFASDGASAGGAPAGGHASAP
                     KRLAIYGFGSAASLCTQVAIWRGWEVYGIARDAEHRAMARELGAVWAGEANDDPGVVY
                     DAAIVFAPAGELAVDALRRLDKGGVVALGGIYSSPIPPIEYPWIYHERIVRSVANSTR
                     QDARELLRDAAAIPIVSRVQEYELEQANEALLDLKEDRVRGSAVLRVAGD"
     gene            23936..24766
                     /locus_tag="VFZ86_03750"
     CDS             23936..24766
                     /locus_tag="VFZ86_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641437.1"
                     /translation="MPTTLVSSVLDAADAEDLVGTVDAAAPGLRRRLGQSLGDALRGR
                     PSAARRYVAAYGELVELLLALAHEARPELVVRDDAGDYCVVHDAGCSRAPAELLRSFA
                     ADAPFSAWVRAGKLGAGVILDLVARLRALLPGTQPLLLPAGGSVPQYTGEAEALLRFT
                     RLVAEAQLAERSDLERVRGVFGLSITELGALFGVTRQAAGLWLTDGPPPARKAKAAAV
                     AAIADLLAHRLKPARVPGIARRPAPAYGRRSMLEVIAADEHEWLLQSVRRSFDYAATA
                     "
     gene            24763..>25248
                     /locus_tag="VFZ86_03755"
     CDS             24763..>25248
                     /locus_tag="VFZ86_03755"
                     /inference="COORDINATES: protein motif:HMM:NF020387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES domain-containing protein"
                     /protein_id="HEX5641438.1"
                     /translation="MRRRDIVDRFLTVVRGGEYVRVADPGWRDPLSGDFARRLGGRWN
                     PPGGFAVVYLNGDARVARANVLARFSGLPYGPEDLEPAAAPVLVSTDVEPDDFADVVS
                     DAGCVAAGLPVTYPAGSGGRTVPHEECRPIGRAAWDEGLPGRRGGSPRAPPSCRRCPW
                     DT"
BASE COUNT         3326 a         9078 c         9206 g         3638 t
ORIGIN      
        1 cgagcggctg ctcgtaggcc tcgccggcca gcatgccggc gacgaccgcc ctgcgccggc
       61 cctgcaggcc ggcggggagg cggtcctcca ccatcgccac gtgccgctcg ccgaggtcgg
      121 cgatgaagtc gatcgccgcc gtcgtccccg ccatcccctc atggttcagc gtgccggtct
      181 cccacacgta cggcgggtcg ggctcctgcg tgctcaagcg gaccggctcc agcgcctccg
      241 tggtctggcg gcgtgcgtac atcaggccca cgtgagggcc gaagaacttg taggccgagc
      301 agagcaggaa gtcgcagccg atcgccctga cgtcgatcgg cccgtgcaac gcgtagtgca
      361 cggagtcgac gacgctgagc gcgccggcct cgcgggccag cgccgccgcg cgcgcgacgt
      421 cgttcacggt gccgacagcg ttcgaggcgt ggccgatcgc gagcacgcgg gtcttgcccg
      481 gctccaccag cttctcgaac gcgtcccagt cgagggtgca ggtctcgagg tccaccggga
      541 cctctcgcac gacggccccg cgctcggcca gccgcagcca cgggccgcgg ttggcctcgt
      601 ggtcgagctg ggtgacgagc acctcgtccc cgggccggat gtcccgcgcg acggcatcgc
      661 tgagcagctg cccgagcgtg gtcatgttgg cgccgaagct cacttcctcc cagtggcagc
      721 cgaggaggtc ggcgccggcc tcgtgcgcgg actgcaccac ggcgtccgtc tcctcagagg
      781 tcgcgaagga tccgtggatg ttgcagttgt ggttgacgag gtagtcgacg acggcgtcga
      841 cgacgcgctg cggcgtctgc gttcctccgg ggccgtcgag gtaagctgcc ggctgtccgt
      901 cgaccatccg cttgagggcg gggaactggc tgcggacgta agcgacgtcg aaggatgaca
      961 taggctcacc tcggagcggt ggtgacagga aggaccagtg taccgtcccg tgcaggaacg
     1021 gcgcacatgg ggcaggatgg gaggacgatg ccgaccgggg gcgacaacac cagacgagtg
     1081 cgcgcgcgca cgcacgagcc gagactcgag cgcgcgctcg cccgccggtt cccggtgatc
     1141 tgcttcgcgt gggacggcac cgccgtggcg cggcgtgaag ccgacgcctc cgcagtgcgc
     1201 agccgcgtgg agcggctgac ggcgctcggc gtcgacatcg ccgtcatcag cagcgcgcac
     1261 gtcacggagg tcgacgagca actgcgcgct cgcccgggcg tcgagggccg cctgttcctc
     1321 tatctctcgc gcggctccga ggtctacgtg gtgggcccgg cgggcccgcg tctgctcgag
     1381 cggcggcagt cgacgccgct ggagaaggag ctgctcgtcg ggatcgcgga ggcgcttcgc
     1441 gaccgcctgg cggccgccgg gctccacgtc gagctcgtgc ggcatcacct gaaccggcgt
     1501 tcggtcgatc tgatcccgga ctggccggag ccgccgggcg cggaggtcga ggagctgcag
     1561 cggcaggtcg aggcgcggct gcgcgacgcc ggcttcgccg gcggtctcaa ggagtgcatg
     1621 gagatcgcgc gccagctggg ccgcgacgcc ggtctccccg agccggccgt caccagcgac
     1681 gtgcgccaca tcgacatcgg cctgaccggc aagggcgacg cgatgcacgc cgtgtcgcgc
     1741 gccctcgtcg ccggccgtgg ccggcagccg caggacctgc tggtgcttgg cgacaccttc
     1801 ggtcccgtcg ccgacggcca gggaacggac tcggcgatgc tcattccgga gctccgccgc
     1861 gcggtctacg tcagcgtcgg agccgaggcc ttcggcgtgc cgccgcaggt gctgcacgcc
     1921 ggcggcggcc cggtctcgtt cctcgacatc ctgcacgatc agctcgcgat gtgggagatc
     1981 gtcgcacaac ggagcttccc cgagccgacc ggcgacccgg cctggcggtt cgaggtcaag
     2041 ggcttcgacc ccttccgcga gcgcgaggtc gagacgtggc tcacggtcgc caacggcgag
     2101 acgggcaccc gtggcgccct cgaagagggc tcggccatct cgacgccggc caccttcgtc
     2161 gccggcgtct acggggacgg gaccggcgac ccgccgttcc gccagccggt cccggcgccg
     2221 gactggaccg gcctcaggct catggtggcc ggcagcgcga tgacgctctc caacggcgag
     2281 atcatcgacc acgagagagt cctcgacctg cgccacgggg tcgtgtaccg gacctggcgc
     2341 cagcggctgc ggagcggacg cacggtgcgc gtgcgcaccg cccggttcgc ctcgctggcg
     2401 gaccgccagc tgatggcgct gcgggcggag gccacgcccg aggacttctc cggagcgatc
     2461 gtgtggcaga gcgccatcgg cgtgacccac gccggcggcc cgaccaggga gacggcgttc
     2521 gagatcctcg acgacccgcc cgggatgatc gcgaggacgc gcgggcgcaa cggcggcggc
     2581 cacgtgctcg ccgtcaccac caatccggcg cccggctcgc ccgtcgcgcg cacgccacag
     2641 caggcgcgcg acgtgatcgg cgggcgcctc gagcccggcg atccggcgac ggtggaccgg
     2701 ctcgcgtcga tcgtctcagc gcgtaccaag gtcccgtcgg ccgacgtcgc gcgccgcgcc
     2761 ctcgagcgcg cccggcgcac gggctacgac gagctgctgc ggcggcacca ggcggcgtgg
     2821 gacgccgcct gggacgccgc cgacctgcgc gtcgacggcg acgccgagga ccagcacgcc
     2881 ttgcgcttcg ccatctacca catgatcagc accgcgcacc ccggcaagga cacggtctcc
     2941 atcggcgccc gcggcctcgg cgggatgtcc tacttcctgc atgtgttctg ggacacggag
     3001 atcttcgtgc tgccgttctt catctacacg cagcccgaga cggccctgac catgctcaag
     3061 taccggtacc gcaacctggc gggggcacgg gagaaggcgg cgcgcatggg ctttcgcggc
     3121 gcgctctacc cgtgggagtc cgccgacaag ggcgtcgaga cgacgccgcc ctacggctac
     3181 gggccgagcg gcgagcaggt ccccatcctc tcgggcctca tggagcatca catctcggcg
     3241 gacgtggcct gggcggtgtg ggagtactgg aaggcgaccg gcgacgacgc gttcatggcg
     3301 agcatgggcg tcgagatgct gctggagact gcccggttct gggtgtcgcg cgccgcgagc
     3361 gaccggcagg gccggtatca cgtgcgcgtc gtcgtgggcc ccgacgagta tcacgagggc
     3421 gtggacgaca acgcctacac caacgtgctc gcgcgctgga acatcaagcg cgcgctggag
     3481 gcgctcgcct ggctggaggg cgtcgaccgg gaccgcgccg tggagctcaa gaagcggctg
     3541 ggcacgacgg gctcggagct ctccacctgg cgcagggtgc tggacggtta cgtgaccggg
     3601 ttcgacccgg agacactgct ctacgagcag ttcgccgggt tctacgacat ggccgacgtg
     3661 ccgatcgaga agctccggcc gcggccgatg gccgccgacc tgctgctcgg ccgcgacgtc
     3721 acgctggtca gcaaggtggt caagcaggcg gatgtggtga tgctctgcca cgtgctcgcc
     3781 gatgagatcg acgaggcgac cacccgcgcc aactacgact actacgagcc catcacctgc
     3841 cacggcagct cgctcagccc gggcatccac gcggcggtcg ccgcgaggct gcacgacatc
     3901 cccgaggcga cggagtactt caagatggcg gcggcgatcg acctcgccga caacatgggc
     3961 aacgcggccg ccggcctgca catggccacc atgggcggcc tgtggcaggc ggccgtcatg
     4021 ggcttctgcg gcatcatgcg ccgcggcgag gcgctgcgcc tggacccgca gctgcccgag
     4081 gcctggaagc gggtgcgcat gcccctgcgc ttccgtggcg cccggctcag ccttgacctc
     4141 cgcgcccgga aggacggagt ggacgtgggt ataacggtgg agaacgcacc ggtcaccctt
     4201 cagctggacg gcgtggaacg ggagttccct cccggacgcc accggctccg gcgacgtggt
     4261 gggggaccct ggcaggtgga ggccggatga cgcttctcgt agccgtagca gacgcccagg
     4321 ccgaccggga gttgatcgcc tcggcgcgcg ctctcgcggc cgcggcccgc tgggagatcc
     4381 gcgcggtgca cgtgcgcgag ccggacgttc ccgagccggg cagcgcagac ctcgaggggc
     4441 tcgagctgag cgccatcgac ggcgatccgg tggagtcgct cacgcggctc gtcggcggcg
     4501 gcagcgaggt cgacgcgttc gccttcggcc tgagttgctc gtgcgccccc gggccggcgg
     4561 cgggcatcgg ccacgtggcg gaggcgctgc tgaacagcca cgtcgctccc gtgctgctcg
     4621 tgcggccggg catgcgtccc gtcgccggcc tcaagcgcct gtacgtgccg ctggagggca
     4681 gcccgtcgtc gtcggccgcc atgcgcgccg ccgacgacgc actctgcgcc cgcggtcggg
     4741 agatcgtcat gctccacgtg gtgaccggcg gcgaaacgcc ggccgagacg ggcagcctgc
     4801 cggcgccgcg catgaccgac caggagcact acgagtggag cgcctggcag gacgagttca
     4861 cgatgcgctt ctcgcagtgc ccggagggcg gccggcaccg ggtcgcggtg cgcgtaggcg
     4921 tcccggcgga gatcatcgcg gaggaggccg gcaagaacgg cgcggagctc atcgtgctct
     4981 cctggaacgg gagcttcgag agcggccacg gcgccgtcat caaggagctc ctcgtcacgg
     5041 cgccctgccc gctgctcctc gtgcccagcg gcgtccccga agcggtctga ggcatcccgg
     5101 cgccttcgcg ccggctgtgc cgcgtccgcg gggcgagcga aggggtcgcc gacccgtagg
     5161 ggtcgcggaa ggaccccgtt gccgctcgtc cggccgatca ctcgtcaagc ctccgtagag
     5221 agcggctgcc tccgcgtaca gattgtgtaa ggaagccgtc cacggacctt gccgaggggt
     5281 accgtcgagc gatgatcgac gtactcctca tggcgcttgc catcgccttc ttcgccgccg
     5341 ccgacctctt cgtccgcggc tgcacccgga tcctcgagcg cggcagcgac gagcatcggg
     5401 aggacgggca atgaccgccg ccgacgtcgt cttcttcgtc atcatcgcgg cgctcctggc
     5461 ctacctcacc tgggcgctga tcgcgccgga ggacctgcag tgagcggtcg ggctcggtac
     5521 gcgcgatgac cacgtcggca tggctccagg ccgccgccct cgtcgggctc ctgctgctcg
     5581 ggacccggct gctcgggccg tacctggcca aggtctacgc gggcgccggc gcacctggcg
     5641 accgcgcatt cctgccgctt gagcgcctcg tctaccgcgc ggcccgcatc gacccggaga
     5701 acgagcagcc gtggaccgtc tacgcgcgct cggtcatcgc cttcagcctg gtctccgtgc
     5761 tcggcctgta cgccctgcag cgcctgcagg gcgggctgcc gctcaacccg acgtcggtcg
     5821 agtcgacgcc gccgtttctc gccttccata ccgcagcgag cttcgtcagc aatacggatt
     5881 tccagaacta cgccggcgag gccacgatga gccacctcgt ccagatggct gggctcaccg
     5941 tgcagaactt cgtgtcggcc gcggtcggga tggcggtcgc cgtggccctg atccgcggct
     6001 tcacgcgaga gcgggccggg accatcggca acttctgggt cgacctgacg cgtggcgtga
     6061 cccgcgtcct gcttccgctg gcactggtcc tcgcgctggc cctcgtcgcc ctcggcgccg
     6121 tgcagacgct ccagggcccg gtcgacgtgg ccaccgtgga gggcgcgacg cagaccatct
     6181 accggggccc gatcgccagt caggagtcca tcaaggaact ggggacgaac ggcggcggcg
     6241 tcgtcaacgc gaactcggcc catccgttcg agaacccgac gggtctcacc aacctcctcg
     6301 agatcttcgc gctgctgctg atccccttcg ccctgacctt tgccttcggg cggctcgtca
     6361 aggaccagcg tcagggctgg gcgatcttcg tcgcgatgtt cgtgctctgg gcggggtccg
     6421 tcgccacagc gacctccttc gaggtgcagg gtaacccccg cgtggaggtg ggagccgcca
     6481 acatggaggg caaggaggtg cgcttcgggg ccgcgacctc ggccttgttc gcggcctcca
     6541 ccaccggcac gtcgacgggc gcggtcatct cctcgcacga cagcttcacg cccggagggg
     6601 gcgccgtgcc gctcgtcaac atcctgctcg gcgaggtctc gccgggcggc gtgggggccg
     6661 gcctgtatgg catgctggtc ttcgtgctgc tggccgtgtt catctccggc ctcatggtcg
     6721 ggcgcacgcc cgagtacctg gggaagaagg tccaggcggc ggagatgaag ctcgtcgtcc
     6781 tgtacatcct ggtggtgccc gtcgccgtgc tcgtgttcag tggggtgtcg gtggtgctcg
     6841 ggcccgccag ggagtccatc ctcaaccccg gcccgcacgg gctgaccgag gtcgtctacg
     6901 cgttcgcctc tcaggccaac aacaacggat ccgccttcgg cgggctcacc ggcaacaccg
     6961 actggtacaa cacgaccggc gcgctcgcga tgctcgccgg caggttcttt ctgatcgtcc
     7021 cggcgctggc catcgccggt tcgctggcgc gcaagaagac ggtgcccccc agcgccggga
     7081 ccctccctac gcacaacccg ctgttcgtgg tcatgctgct cgcggtgatc gtcatcgtcg
     7141 tcgggctgac ctacttcccg gtgctggcgc tcgggccgat cgtggagcag ctggcgctgt
     7201 gacccgcaag acctccatgg tcgatcgcag gctgatgcgc cgggccgcgc aggatgcggc
     7261 gatcaagctg gatccccgcc gcatggcgcg caaccccgtg atgttcgtgg tcgaggtgac
     7321 cagcgtcctc gtcacggcgc tcgccgttct cgagccgagc gtgttcgcct ggtcgatcgc
     7381 cgcatggctg tggttcacgg tgctgttcgc gaactttgcg gaggcgatgg ccgagggccg
     7441 gggcaaggcc caggccgagt cgcttcgccg tacccgatcg gagacgacag cgcaccgtcg
     7501 cggcgaggac ggcggcctcg aggacgtgcc gtccagtggc cttgcgcttg gtgacgtggt
     7561 cgtcgtcgcg gccggagagc tcatcccggc cgacggagag gtcatcgagg gcatcgccac
     7621 cgtcgacgag tcggcgatca ccggtgagtc ggctcccgtg atccgcgagt cgggcggcga
     7681 ccgctccgcg gtcaccggcg gcactcgcgt gctctcggac cagatcgtcg tgcgcgtgac
     7741 gcaggagcct ggcgcgagct ttctcgaccg catgatcgcg ctggtcgagg ggtcctcgcg
     7801 ccagaagacg cccaacgaga tcgcgctcga catcctcctg gcgggcctca ccgtcatctt
     7861 cctgatcgcc gtggccaccc tgcagccgtt tgccgtgtac tcgggggcac gccaggacgt
     7921 catcgtcctc gcggcgctgc tcgtatgcct catccccacc acgatcggcg cgctgctctc
     7981 ggccatcggc atcgccggca tggaccgcct ggtgcagcac aacgttctgg cgcgttccgg
     8041 tcgcgccgtt gaggcggccg gcgactgctc aacgctgctg ctcgacaaga ccggcacgat
     8101 cacgctcggc aaccgccagg cggcggagtt cctgccgctg ccgggcgtga ccgagctgga
     8161 gcttgccgac gcggcgcagc tctccagcct ggcggacgag acgccggagg gccgctccat
     8221 cgtggtcctc gccaaggagc gcttcaacct gcgcgagcgc gagctctcgg gcgcgacgct
     8281 catccccttc accgcgcaga cgcgcatcag cggcgtcgac atcgacggcc gctcgatccg
     8341 caagggcgcg gcggaggccg ttcggcggct tctcgccgac ggcgggcagg agccgccccg
     8401 cgagctcgac gagctcgtgg acggcgtcgc cgccgccggc gggacgccgc tggtcgtcac
     8461 cgagaacggc tgcgccctcg gcgtgatccg gctcaaggac gtcgtcaagg acggtatgcg
     8521 cgaacgcttc gacgagctcc ggcgcatggg catccgcacg gtcatgatca ccggcgacaa
     8581 tcccaggacc gcccaggcca tcgcggagga ggcgggcgtc gacgacttcc tcgctgaggc
     8641 gacgcccgag gacaagatgg cgctcatccg cagagagcag gccggcggcg cgctggtcgc
     8701 catgaccggc gacggcacca acgacgcgcc ggccctggcc caggccgacg tcggcgtggc
     8761 gatgaacacc ggcacgcagg ccgcgcgcga ggccggcaac atggtcgacc tggactccaa
     8821 ccccaccaag ctcatagaga tcgtggagat cggcaagcag ttgctcatca cgcgcggctc
     8881 gctcacgacc ttctcgatcg ccaacgacgt cgccaagtac ttcgccatca ttccggccat
     8941 gttcgccggc gcgttcccgg tgctcgacgc tctcaacgtc atggacctct ccaccccgca
     9001 gtcggcgatc ctgagcgccg tcatcttcaa tgcactcgtc atcgtcgccc tgatccccct
     9061 cgccctgcgc ggggtcaggt tccgcgccgt gtcggccgcg agcgcactgc agcgcaacct
     9121 gctcgtctat gggctcggcg gcatcgtggc gcccttcgtc ggcatcaaag tcatcgacgt
     9181 cgtcctctcg gccttcggag tcgtgtgatg cggcgccagc tccccaccgc catcgtggtg
     9241 ttcgccgtct tctcggtcct cgtgggggtg gtgtatccgc tcgtcgtgac cggcatcgcg
     9301 caggtcgcgt tcgggtcgaa ggccgacggc tcgctggtcg tgcgcgacgg cgcgcccgtt
     9361 ggctcttcgc tcatcggcca ggcgttcgag ggtcccggct acttccaccc acgcccgtct
     9421 gcggccggcg acggctacga cgcgatggcc agtgccccgt ccaacctggg gccgagcgat
     9481 ccgggactcc tcgacgccgt ggcgcagcgc aaggaggagt accgggagga gaacggcctc
     9541 ggcgccggcg agcgcgtccc ggtcgaagcg atcaccagct cgggctccgg tctggacccg
     9601 cacatctcgc cggccgacgc ccgccttcag gctcggcgtg tggcggaggc tcgcggtgtg
     9661 tcgctgaccc gcgtgctagg cttggtcgac gaggcgactg aggggcggcc gctcggtatc
     9721 ttcggcgacc ccggcgtgaa cgtcctcgag ctcaacctgg cgctcgacga gcgccgctag
     9781 tggagggcgg catgagccgc ggcacgctgc gcatctacct cggcgccgct cccggcgtcg
     9841 gcaagacgta cgcgatgctc aacgagggtc gccggcgcca gacgcgcggc gaagacgtcg
     9901 tcgtgggctt cgtcgaatcg cacggccggc agcgcacctc ggagcaggtc ggcgatctgg
     9961 aggtcgtccc gcgggcgccc gtcgcgtacc gagaggccgt cttcgaggag atggacatcg
    10021 acgccgtcct ggcccgccgg cctcagctgg cgctggtgga cgagctcgct cacaccaacg
    10081 tcccgggcag tcgcaaccag aagcgctggc aggatgtgga ggaactgctc gacgccggga
    10141 tcagcgtcat ctccaccctc aacatccagc acctcgagtc gctgaacgac gtggtggagc
    10201 agatcaccgg ggtcgtgcag cgcgagacga tcccggacga aaaggtgcgg cgagcggacc
    10261 agctgcagct ggtcgacctc gccgtggaga cggtccgcgc gcggctcgcg cgcggcgaca
    10321 tctaccccgc cgagcgcatc gacgccgcgc ttggcaacta cttccgcgcg ggcaacctgt
    10381 ccgcgctcag ggaactggcg ctcctctgga tcgccgacag ggtcgaagaa ggcctcgaag
    10441 agtaccgggc ccgtcacggg atctccgaga cctgggagac gcgcgagcgc gtgatggtcg
    10501 ccctgtccgg ctccgccgac ggcgaccggc tggtacgccg cgccgcgcgc atggcacagc
    10561 gcctcaacgc cgagctgatc gcggtgcacg tgcgcccgca gaccggcctg gcgacgcagt
    10621 cggccggtcg tctggagcaa cagggacgcc tggtggagcg actcggcggc gcttaccgcg
    10681 aggtctcggg cgccgacgtc ggcgaggcac tggtcgacac ggcgcgagct ctcaacgcga
    10741 cgcaggtcgt ggtcggagcg tcggagcgtt ctcctttgag cctgcttctg cacggctcgg
    10801 tggtcaggac ggtgatcagg ctctcgggtg tgggcctcga cgtccacgtg atcggccgcc
    10861 aggccgacgc cgagccggag gcggcgcgct cgcagcgact gcgcgctccg cgcgcgctgc
    10921 cgcgccggcg cgtggcgctg ggcttcgtgc tcgcggtggt cggcgcgccc cttctcgcct
    10981 gggccctgac gcacgcgcgc gaccaggtgt ccccatccgg cgcactcctc ctgttcctgc
    11041 tgctcgtggt cgcggtctcc gccctgggag gcctctggcc cgccctcctg gccgcgctcg
    11101 gcggattcct gctcgtcaat tggtacctga cgccgccgct gtacacgtgg acgatcggcg
    11161 acccggagga cgtccttgcg ctcctggtct tcctggcggt ggccgttgcg atgagcttct
    11221 tcgtcgcgct ggccgcccgg agggctgccc aggcgacgcg cgcccgcgcc aaggcggaga
    11281 cgctgatgcg cctcgcggga tcctcgagca tggaggcggt gctcgagggg ctgcgccgct
    11341 ccttcggctt cgagagcgcg gcgctgctcc accgcgacga ccacgagtgg acggtggagg
    11401 cagcgagcgg ctccgcgccg ccttcgctgg ataccgcgga gctcgtcgtc gaggtcgacg
    11461 cggggcacgt gctggtcgcg tcgtctccgg cgcctcgccg gaccgaggac gtgcgcatgc
    11521 tggaggcctt tgccagggag ctggcggcat ccgtcgagca cgaggagctg gaggcggagg
    11581 tggccagcgc cgaagggctc gcggcggtca acgagctccg cgcaagcatc ctctcgagcg
    11641 tctcccacga cctgcgcacg ccgctttcgg ccatcaaggc ctcggcgacg agcctgctcc
    11701 aggacgacgt ggactggacg ccggaggcgg agcgcgagct gctgacgacg atcgacgagg
    11761 agaccgaccg gctgaaccgg ctggtcggca gcctcctgga catgagccgc ctgcaggccg
    11821 gagcgcttga ggtacaggag gaggtcatca tcctcgacga ggtgctcgcc gaggcgatgg
    11881 cgagcgtcgg cgccgggagc accgaggtag tcctggatct ccccgaggac ctgccgccgg
    11941 tggtcggcga cgaggcgctg ctggagcggg cgctggccaa catcgtcgcc aacgccgtca
    12001 cctactcgcg cggcgagccc gtgctcgtga gcgccggtgt ggtgcccgat ggcgtcgacg
    12061 tgcgggtcgc cgaccggggc cccggcgtcc cactgcagga gcgggagacc atcttcctgc
    12121 cgttccggag ggtcggcgac acccgcaggc acgagggcgc aggacttggg ctggccatcg
    12181 ccaagggctt cgtggaagcg atggggggca cgatcgaggc ggacgacacg ccgggagggg
    12241 ggctgaccat cgtggtgcac ctgaaggtca tgaagtgacg cgcgtcctgg tcgtcgacga
    12301 cgaaccgcag atcctgcgcg cgctgcgcat caacctgcgc gcgcgcggct acgaggtgga
    12361 cgtcgcggcc gacggagagt ccgccctgcg gctcgcggct cgcgatcacc ccgacgtcgt
    12421 cgtgctcgac ctcggcctgc ccggcatcga cggcatcgag gtgatcgagg ggttgcgggg
    12481 atggacgtcg gtgccgatca tcgtgctctc ggtgcgcgac cgtgaggccg acaaggtcgc
    12541 cgcgctcgac gccggagccg acgactacgt caccaagccg ttcgggatgg acgaactcct
    12601 cgcgcgcatg cgcgccgcgc tccgccgcgt gactcccgcc gacgagccgg tgctgatcga
    12661 gaccccggac ttcacgatcg acctggccgc ccggcgggtg acgcgcgccg gcgaggaggt
    12721 gcgactcacg gccacggagt ggcacatcct cgaggtcctc gtacgcaacg ccgggcgtct
    12781 cgtgacgcaa cgccagctct tgcaggcggt ctgggggccc aagtacgaga cggagacgca
    12841 ctacctgcgc gtgtacgtgg gccagctgcg ccgcaagctc gagccggtcc cgagcgagcc
    12901 gcgctacttc ctcaccgcgg cgcgcatggg gtacaggttc gagcccgagg ccccgtgagc
    12961 ctcgccgcag tgtccgcctg acgctacgcc gcgcgcgcgg cgcgccgctt ctgggcgcgg
    13021 ctcggccgag ccgccgctcc caccagcacc gccggcctcc cccggaacac cgcgatgagc
    13081 gtcaccccga tgaggatgct gacgatgctc acccactgcg gctgggtcag cccgccgaac
    13141 ccgacggtgt tgatgcgcac gaactcgacc aggaagcgct cgatccccga gagcaccagg
    13201 aaccagccga aggtccacca gccggacttg tcgtagcgtg tcgccagccg ccagaggatc
    13261 cagaccacga aggccatggc gatgacctcg tagacgggcg tggggtggac ctccacgccg
    13321 ggcggcgtgg ggacggtgcc gttgggatag cccatcgccc agggcaggtc gctgggcttg
    13381 ccgtagtcgc cgtcgccggc gagctggcag ccgatgcggc cgatgaggta gccgaaggcg
    13441 gtggccggag ccatggcgtt ggcgatgacg tcgagcgggt agcgccggat gagcgtccag
    13501 ccgaccaccc cgaggaagcc gccgatgagg ccgccgtacc aggtgaagcc gatgccgccg
    13561 aaggcggcgc tccagaagtc gtccctgatc agggcgtagt tctcgatgag gtagtagatg
    13621 cgggcgaaga tgaagccgcc gacgccggcg gcgatgatga gctcgtaggc gaactccccc
    13681 ggcacccccc ggcggcgcac gagcggccgc acgatggtca tgccgggcac gatgagcgcc
    13741 agcgccgcca tgaggccgaa gctgtagaag cggtagtcgc cgatctggaa gaggacgggt
    13801 cgcatcgcgt cagtgtaggg ggtgcggcgg ccggcgacca gccgtggggc cggcggaagc
    13861 gcggcgcccg ggcgggccgg cgaaggcctc gcccgccagc tcctccacca gcgccacgtc
    13921 gacgtcgccg ttgtacggcg agacggcgtt ggcggcgcct gcggccgccg cgagacgcag
    13981 cgccgcggcg gtcccctcgc cgcgctgcag cgcgagcgtc aggccggcgg cgaagcagtc
    14041 gccggagccg accgggttca cggcgcggac ctgtcgcgtc gcgacgcgcc actcggcgcc
    14101 gtccacgact gctgcggccc cggcggcgcc cagcgtcagg acggcgccct ccgctccggc
    14161 gtcgatgagg cgccggcaga gctcgaggcc ttcggccacg agcgcctccg gcgcggcatc
    14221 ctgctcgtcg tcgcagcgcg ccccgggcgc gcccaccgcc gagcaggcct ccgccaggtt
    14281 caccttgacc aggtcggggc gctcggccgc agctccggcg agctgctcgc cgtcggcgtc
    14341 gaccaggacc cggtagccgg cggcccgggt ggtgctcacg agggcggcgt agaacccggg
    14401 cgaggcgccc ggcggcgccg ccccggtgac cacggcccac tcgccgcgcc cggcgtcgcg
    14461 cccgcccgcg agctccagca gggcccgttc gtcggcggag gagatctccg ggccgaactc
    14521 gtacaggcgc gtgacggacc cgtcgtcccc caggacgacg gtcgagacgc gcgcctcacc
    14581 ctccgtcgcg accactgcga gctcgaggcc ctcttcgcgc gcgagctcgg cgatgagccg
    14641 cccgccgcgc ccggcggcga agcccgcgag ccggccgtcg ccgcccagcg ccagcgtcgc
    14701 gcgcagcacg ttggcgccct tgccgccggc ctgcgtgcgc agcgcgcggc agcgcgtcat
    14761 cccccccggc tgcggagggc ctccggcgag catacggtcg atggcgaggt tggggcagat
    14821 gacgagcgtc atgaccctcc cggggtcggc ggccgcgggc agtgtctcag cgcttgaggt
    14881 gcacgtatcc tttgccgtcg gcgatcttga cgttggtgtc gagcgccgag gagaacccct
    14941 ggaaccgctg caggatgctg tcggcctgct ctcccttgac cacgccgggg acgcccgagc
    15001 cgccgccgac caccgggatc aactgcgcgt cgatgcgctc ctccgagacc ttggcgcgca
    15061 cgagcacggt ctccgcggtc tcgccgctga acccgctgaa gacgaggttg ccgaggctgt
    15121 agaagatgag tccgccgtgg taggcctcca cgccgttgag caggtgcggg tgatgggcga
    15181 agacgatatc ggcgccggca tcgacggcgg ccttcccctc gctctcctcg agggcgcttg
    15241 gcgcgcgctt gtactcgaag ttccagtgcc agccgacgat gacgaagtcc gccttcttcg
    15301 cggcggccct gatcgccttc gtgacggcgc cgatgtcggc gcggccgggc gccacaccgg
    15361 ggctcgacga ggtggcgggg tagccgacgg gcaggacgtc ggtgaagccc aggaaggcga
    15421 ccctgacacc gtcctcggtc tcgagcacgg tgcccttcag ggccgcgtcg aggtcctcgc
    15481 cggcgccgac gacggcgatg tcgtacttct cgcagtactc gatggagtcg agcaggccgc
    15541 tgtcgcccat gtcgccggcg tggttgttgg ccatcgtgac cacgttgatg ccgctctccg
    15601 ccatcgccgg cgcgaggtcg gggttgccct tgatgacgac gtccttccag ccctgcgctt
    15661 cgccgccggt cgtgagcggg ctctcgagat tcacgaagcc gaagtcgctc ttctcgaagg
    15721 tcggcgcgat gctctcgaag aggccgctgc ccatgctctc gaccactccg ccgacgccga
    15781 acccgccgat gacgtcgccg ccggccgtga ccgagaaggt gggggccgca gccggggtcg
    15841 gactgggcga cgcggatggc gaaggttccg cggagggcgc cgaggtcagg tccgccgcgc
    15901 cggcgccggt ggcggccggg ctcgcctgtt cggcggcggc ctcgcccccg ccgccgaagg
    15961 gtccccagcc ggcggcggcg gccacgccgg cggccgccgc gacgatgatg ccgagaacga
    16021 tgagcaggag gacgaagccg ccgccccggc gcccacggcg gcgaccccgg tcgccgcggt
    16081 cccgtggcgg cccgccatcg ccggacggcg cgcccgccac gtcctcgtcc ggagtcgtgc
    16141 cggcgcggta caggctgtac ggcttgtcgt gtccggacgg cgtgctcatc ggtcacatag
    16201 tacacgggcg gcccggcgcc cgcgtgtggg tccgccgccg tgggcgccgc cgccgcgggc
    16261 ggcaggtcgg gtccgccgct tccctgggtc aagggagcac ggcacctggg cgataagatg
    16321 agtcgaccct gagaatccgc cgcggaggcc gcgtgcgcgc agcacaccgt atcgccagtc
    16381 tggtcctcgc caccgtcgcg gccgcgactc tctgggctgc ggccgcgggc tgcgggatcg
    16441 acctcggcgc gtccgatgac gacgcccggg ccgacctgga gcggacgctg cgtctggctc
    16501 gccacgagtt cgccgccgcc gcggatgcgc tggcggccgg cgaccgcgcg gcgttcctcg
    16561 actggctgcc acacgacagc ggcagcccgg gatcggtcgc ggccagagag ggcctcgccg
    16621 agctgttcga cagactcgcg ccgctgccgt ggcgcacgtt ctcctttgag gtcacgccgc
    16681 tggacccggc ggagggggtc ttccgggtcc ggggcatcgg gcagctcggc gacgccggtc
    16741 ctcccgacag gatcgccgtc gtcaggtact tcaggctgct cgccgtcgcc gacggcgcgg
    16801 tcgtgctcgc cgacgagaca ccggaggacc tccgccgccg gtacctgatg gcgctgcacg
    16861 accccctcgt gctgcagcgc ccggggctca tcgtgctcgg cgacgcggcg gcgcgcggac
    16921 gggcggccgc ggtgatggcc gcggccgcgc gggcgcggcc ccagctcgcc gccctgggcg
    16981 tcggcgccgg cccgtccgtg gtcatcaccg tgtacgggtc cgctgcggac gtgcgcgacg
    17041 cgctcgctct cggagcctcg acggcgcgcc tcgtcttctt ctcccacccc tccctgcgcg
    17101 tagccgagga ctcctggccg acctacgacg tcggcgtgat gggcccgtgg ctgcgtgatc
    17161 ggggcggttc catggacgcg gtcctgcggc acgagctcgc gcacgcgtac acggtgagct
    17221 ggttcggcga cgacgaggag cctccggcgc tgctcgtgga ggggatcgcc caggcggcag
    17281 agggaacggc cgccacgccg gccctgcggc aggaggtggc caccggcgac cagctctggc
    17341 cgctgccgga gtcgttcgcc gacaccgacg tgtgggacgg cggcgacgcc gcggcggtgc
    17401 ggctgggcta cgagatcggc ggggcgctcg tcgcctacgt cgtctcgcgc tggggtccgg
    17461 agcagctgcg gccgttcgtc caggcgctgg ccgcggccga gccctccgag gcgggcatgg
    17521 acgcggcgct cggcggcacg ctgggcgtga gctggcgcga gttcttcgcc ggctggcgcg
    17581 actctgttct gacgggagac tgagggcgga gtccccggcc ggcgtgcggc cgtacaatga
    17641 cggtatgacc gcaccgtccc gcagagcccg gcccgccctc ctcgtcgcag gggcgggcct
    17701 cgcgatcctc ctcgtcgctc tcgtcgcggc cggagtgggc ctgtcgcgcc gggaagagcc
    17761 ggcccagccg cgagcggcgg cgccgagggt caccacccgg gacgtgcagg cggcgctcga
    17821 cgacgcgacg ccggcgctgc tgcagcggga ccgggcggct tgggacgccg ccttgcctgc
    17881 ctcgggcggc gacgcccgcc acggcgtcga cgtgctgtac cggcgcctcg cgccgctccc
    17941 gtggacggac ctgcggctga tcgcggaggc ggtgcgcggc cggcccggcc gcttctacgt
    18001 cggcgcggtc ggcgaggtcg gtgacgccgg cccggccgac cgcatcatgg cgcgccgcgt
    18061 gttcgacgca gggctccgcg gcgggcgggt cgtgctgcgc gacgacgtca cgccgcgcga
    18121 cctgcgcggg cagggcgtca tgacgttcgc acggcctgtg gcggtgcgca ggcacggact
    18181 cgtcgtcatc gccgacgagc gcgagcggcc cgcggcagag gctctggcgg cgacgggcgc
    18241 gacggcccgt gaacgcctcg agctgctcgg catcagcgcg agcaagccga tcgtcgtgta
    18301 cttctactcg tcccggcggc agctgctgcg ctcgctcgga gaagaccccg gcgaggcgcg
    18361 catccgtttc ttctcgcacg ccccgaagcg gctgcgcgac gtacccacct ggacgcgcga
    18421 catcggggtg ctgggccccg ccctgcgcgg caaggcctcc tggacgccgc ggatgctcgc
    18481 ccacgagatg acccacgcct acacgtcgcg ctggttcgag ggcacgagga acgcgcccac
    18541 gctgctcgcc gagggattgg cgaccgccgt cgaagggggc cgctcgttcc agccgttgcg
    18601 agacgacctg gcctcccccg agtcggcgtt cccgctggag aaggcgctca aggcaaagag
    18661 cctgtggaag ggcaaccgca tcgcgaaggt gcgactcgcg tatctggagg gcgcctcgct
    18721 ggtgctgtac gtccgcgacc gctggggcct gcgcgggctc agggagttcg tcgccgcggt
    18781 cagcgattcg gacctcacct cccagggtct cgacgaggcc gcgcggcgga gcgtcggcgt
    18841 cggctggaag gcgctgcgcg cgggctgggc ggcgtacgtc gagacactgc cctgaccacg
    18901 ccggcctcgt ctcggacggg gcatccgtgc ggcagcgcgt acccgcgtaa ggttgcgcgg
    18961 ccgggggcgc gggggtacac cctagtcagc agccgctcgc tgctgcagcg cgaaaggaga
    19021 agtgcatgcg ctccagggtc ctcatcctcg ccacgctcgt cggcctcgcg gccgtcgtcc
    19081 tcgtgatcgc cgtcgccgtc gccggcgccg ggcagtccga tccgctcccc gacatctccg
    19141 ccccggacct cctcgggaag atggcgcaag ccggagacgt gaccgcggtc agcggcgacg
    19201 tcgcctggca gaacgacctc ttcggcgacc tcgcacaggc gagcggtatg gctcagttgc
    19261 ctgcgcagtc gccgctcacg agcagcgggt cgggccgcat ctgggtgagc gacgccggcg
    19321 cacgggtgga gtcgcagggc ggcggcggcg atcaggtggt cgtcgtggac aaggccgcgc
    19381 gtacggcttg ggtctatgac tacgcgcaga acacggtcaa gaaggtcgtc gtgaccggga
    19441 cggcgccggc ggagactccg tctccggcgc ccgccgccac cgcgctcacg ccggcgatga
    19501 tcaccctctc cctgcagcag gccgcccgct tcgcgaccgt cgaggtcgcc ggccagacca
    19561 aggtcgccgg gcgagacgcc taccagctgc gcatgacgcc cgtcgccacc gacaccgccc
    19621 tcggctacgt cgaggcggcc gtggacggcg agacctcgct gccgctgcag ctcgacgtgt
    19681 acgccagggg cggcaccgtg ccggtgctgc gcttcggctt caccagcgtg agctacgacg
    19741 ccatcgaccc ggcgacgttc gccttcaccc cgccggaggg cgcgcaagtg acgacgagga
    19801 cggtcgacgg cgacgagatg cgccgtgaga tgcagcgcgc gcacgagggt gacgccggca
    19861 cggagccgac cgccgagcag aagacggccg ccgagaaggc agtgcgcggc gcgctgctca
    19921 cgcgcgagca ggtccaggag ctggtgcctt accagctggc atgggcgcgc gactacgcgg
    19981 cccggccgtt ccgctggggc tacgtcctcg gcccggccgg cccgctcacg ggatccggcg
    20041 cgccgctcat ggaactgatg ggcgccgcca cgggcatgga catcgacgcc ctgtcgccgg
    20101 ccggctccca ttctgtccgt gagggctccg cggccggccc gagcagcgcg ctgctctatg
    20161 gggacgggtt cggcgccatc actctcggcc agaccaggac caccgccgag ctggagaagc
    20221 agctcgccga ggcccgcaag acctcgcaga tcctcggcaa ggccaccgtc ggcggagccg
    20281 aggccctgaa gatcggcacc cctctcggcg gcgtgatcgt gtgggagaag gacggcacca
    20341 cgctggtggc cgccggcatg gtgcccatga gcgatctgga ggcgttcgca agctccgttc
    20401 gttgacgtgg aacaccctga gacgccagac gtcgcggaag cgcaaacgtt gagtgaaccg
    20461 gagggggcgc ggcccgggca gcagagctgc gacggccgcg ctcccatcct cacgacccac
    20521 gccctcacca agcagttcgg ccgcctgcgc gcggtcgacg gcctcgacat gcaggtctgc
    20581 aagggcgacc tgttcggctt cctgggcccg aacggcgccg gcaagacgac ggtcatccgg
    20641 ctcgtcctcg gcctcatcca ccccaccagc ggtcacgccg aggtctgcgg ccaccgcgtc
    20701 cccggcgagc tgcagggcgc cctgcgacac gtcggcgggt tcgtggacga cccgacgttc
    20761 tacccgctca tgagcgcgcg ccggaacctc cgtctgctcg gcagcatgac cggcccggtg
    20821 agcgaggagc gcatcgacga ggtgctcgag atcgtcgcgc tctccgaccg ggccgaggac
    20881 cgggtgggcg gcttcagcca cgggatgaag cagcgcctcg gcatcgccca ggcgctgctc
    20941 cacagccccg agctcatcgt gctcgacgag ccgacgagcg gcctggatcc gcgcggcatg
    21001 aaggacgtgc gcgagctcat ccgcgacctc ggcgccgccg gcaccacggt cttcctgagc
    21061 tcgcacctgc tgcacgaggt ggagcaggtg tgcacgcgcg ctgtgatcat caaccggggc
    21121 cgcgtggtgg tgcaggggcc ggtcagcgag ctcaggccga agaacgacgc gatcaaggtg
    21181 ctcaccggcg accagggccg cgccgccggg gtgctgcgcg gccagttcgg cgccggcgag
    21241 gtgaaggagg acgagggctt cctcgtcgtc cgggcggacg aggacgccgt gcccgagatg
    21301 gtccggcgtc tggtcgagga cggcgtcgag gtgcgcgccg tggtcccggc gtcggagcag
    21361 gggctcgagg acttcttcct cgagctcacc ggcaccgacg cccccggaga gccggcgggg
    21421 ggcaaacgac gcgccggcct cgcgagactg cttcccggag gccggcgatg agcgccgtgc
    21481 ccggtccgtc cggctccgcc cctcctcgcg cccacggcag caccgccgcc ggcatgcgcg
    21541 gacacgcctg gggcgccctc cggcgcgagt ggatcaagct gctgttccag cgccgcagct
    21601 acctcatctg gggcggcgcg ttcctgatcc cgttcctgat ctcgctggcg ctgtacctcg
    21661 cgaagtcgga cccccacggc ggcgacggcc cgctcttctt cgagcgcatc acgtccaacg
    21721 gcatgttcgt caccctggcg tcgctctccg cgctgctccc gttcctgctg ccgatggccg
    21781 ccgccatggt ggccggctac atggtcgccg gcgaggcgga gttggggacg ctgcgcatcg
    21841 tcctgctccg gccgacccgg cgcggcgcct tcatcctggc gaagtggacg atggccgtcg
    21901 tctacctcgc cgtgggcttc gccgtcatgg tggccggcgg tctgctgttc ggcggcgtct
    21961 tcttcgggct gcacccgatg atcacgctct cgggcagcac ggtcggcgtc tggcagggac
    22021 tggggctgat cgtgctcagc tgcctctacg gcctcgccgc gatggcctgc atcgtctcga
    22081 tggccctgct gttcagcacg ctgaccgact cgagcctgac cgcgctcatc gcgacgatcg
    22141 tcatctacat cgtgatcacc gtgctcatcc agttcagcta cttcgactgg ctccggccgt
    22201 gggtcttccc gcagtacctg acggaattcg tcaacttctg gcgcgacccg atctactggc
    22261 gccccatcct caaggggctc ggggtcttcg cgctctggag cggggggctc acgctggccg
    22321 cctatctggt cttccggcgc aaggacgtgc tgtcgtaggg tacaagacgg gtatgccggc
    22381 actcgtcaaa gccagtgtgg ctctctttct ggcggcggag gtcctcatcg tcgccgccat
    22441 cggcgccgac gcgatcttca tggtcatcgc gtgggcggtc gtcgccgcgg cgacggccgg
    22501 cgtggcgctg gtggtgcacg cctccgccgc ccgcgtcctg ctggcggcgc tgctggtcgc
    22561 ggcgtgcgtc ctcttcgccg tggagctcgg tctgttcttc ctcccggccg ccgcggcgct
    22621 cctggcggcg gccgtctccg accagcagca acacgatcac caccaccacg ggatccttca
    22681 cggaagggga tgaggacatg gcaggcacgg gcgccccggc gaccatgcgc gcgatgcagc
    22741 tggcggcgcc ggctcccgtg gccgaggcgc cgctccggct ggtcgagctg ccggtacccg
    22801 cgccgggtcc gggcgaggtc ctcctgcggg tgctggcctg cggcgtctgc cgcaccgacc
    22861 tgcacatcct ggagggcgag ctcccgccgc accgctcgcc cgtggtcccc ggccaccaga
    22921 tcgtcggtga ggtggtggcg ctcgggcgcg gtacggagct cgtcgctccg ggaggcgagc
    22981 cgggcgcgcc ggcgctcgcc gccggcgacc gggtcggcgt gccctggctg caccggaccg
    23041 acgggagttg ccggttctgc gcgcgtggcc gggagaacct ctgcgagcac gcgctgttca
    23101 ccggctggga tgtcgacggc ggcttcgccg agtacacggt ggcgccggcc gggttcgtct
    23161 accgcctccc ggccggcatg acggcgatgg cggcagctcc gctgctctgc gccggcatca
    23221 tcggctaccg ctgcctgcgg ctggtcggca tcgtcggaga cgacggcgcg ctgccgggcg
    23281 gggtcgcggc gttcgcgagc gacggcgcgt ccgcaggagg cgcgcccgcg ggcggtcatg
    23341 cgtccgcgcc gaagcgcctc gccatctacg gcttcggctc ggccgcctcg ctgtgtacgc
    23401 aggtggccat ctggcgtggc tgggaggtgt acgggatcgc ccgcgacgcg gagcaccggg
    23461 cgatggcgcg cgagctcggc gccgtctggg ccggcgaggc caacgacgac cccggcgtcg
    23521 tctacgacgc ggccatcgtc ttcgccccgg ccggcgagct cgccgtcgac gccttgcgcc
    23581 gcctcgacaa gggcggtgtc gtggcgctgg gcgggatcta ctcctcgcct atcccgccca
    23641 tcgagtaccc gtggatctac cacgagcgca tcgtgcgcag cgtcgccaat tcgacgcggc
    23701 aggacgcccg tgagctcctg cgcgacgcgg ccgccatccc catcgtgagc cgcgtgcagg
    23761 agtacgagct cgagcaggcc aacgaggcgc tgctcgacct caaggaggac agggtgcgcg
    23821 ggtcggcggt gctccgggtc gccggcgact gatggcgccc gtccccgggt gaggatgcgg
    23881 agacagcgct tgcgtttcgc ttgcgcttgc ggttcgcttg cgctacgctg acatcatgcc
    23941 gacgacactc gtgagcagcg tgctggatgc ggccgacgcc gaggacctgg tcgggaccgt
    24001 ggacgcggcg gcgccggggc tgcgccggcg gctcggacag tccctggggg acgcactgcg
    24061 cggccgtccc tccgccgcga ggcgctacgt cgccgcctac ggcgagctcg tcgagttgct
    24121 gctcgccctg gctcacgagg cgcgccccga actcgtcgtc cgcgacgacg ccggcgacta
    24181 ctgcgtggtc cacgacgcgg gttgttcgcg ggcgccggcc gagcttctgc gctcgttcgc
    24241 ggccgacgcg cccttctcgg cgtgggtgcg tgccgggaag ctcggcgccg gcgtcatcct
    24301 ggacctggtc gcccggctgc gtgcgctgct tccgggcacg cagccgctcc tgctgccggc
    24361 cggcggctcc gtgccgcagt acacgggtga ggccgaggcg ctgctgcgct tcacgcggct
    24421 cgtcgccgag gcgcagctgg cggagcgctc cgacctggag cgcgtgcgtg gggtgttcgg
    24481 cctgagcatc accgagctcg gcgctctgtt cggcgtgacg cgccaggcgg ccggcctgtg
    24541 gctcaccgac gggccgccgc cggctcgcaa ggccaaggcg gcggccgtgg cggcgatcgc
    24601 cgacctgctg gcgcaccggc tcaagccggc gcgcgtgccg ggcatcgccc gccggcccgc
    24661 gcccgcgtac ggccggagga gcatgctcga ggtcatcgcc gcggacgagc acgagtggct
    24721 cctccagtcg gtccgccgct cgttcgacta cgcggccact gcgtgagacg tcgcgacatc
    24781 gtcgatcggt tcctcaccgt tgttcggggc ggagagtacg tacgcgtcgc cgatccgggc
    24841 tggagggacc cgctgagcgg ggacttcgcc cgccggcttg gagggcggtg gaatccgccg
    24901 ggcggcttcg ccgtggtcta cctgaacggg gacgcgcggg tcgcgcgcgc caacgtcctg
    24961 gcgcggttct ccggtctgcc gtacgggccc gaggatctgg agcccgccgc cgcgcccgtg
    25021 ctggtgagca ccgacgtgga gccggacgat ttcgccgacg tcgtctcgga cgccggctgc
    25081 gtcgcggcag ggctgccggt cacgtatccc gcaggctccg gcggcaggac ggtgccgcac
    25141 gaggaatgcc gccccatcgg ccgggcggcg tgggacgaag ggctgcccgg ccgacggggc
    25201 ggcagtcctc gtgcgccacc gtcctgccgc cggtgtccgt gggatacg
//
LOCUS       DASUMX010000079         7611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1459727, whole genome shotgun sequence.
ACCESSION   DASUMX010000079 DASUMX010000000
VERSION     DASUMX010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 7611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7611
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1459727"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="VFZ86_03760"
     CDS             complement(<1..468)
                     /locus_tag="VFZ86_03760"
                     /inference="COORDINATES: protein motif:HMM:NF014088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF123 domain-containing protein"
                     /protein_id="HEX5641439.1"
                     /translation="MTAQVDPRLYIVLTRVPRRSTLQVGSLGPVTLERGWYAYVGSAA
                     RAREARVARHLAREKQLRWHADYLFTAFPAGRAWLVDGATGECALAGALAALPGAERR
                     PRRFGAGDCRCHGHLVRFARRPLRRDLTAAAHAAGAPATGAVRAFRLRSAPRTV"
     gene            complement(465..1418)
                     /gene="mutM"
                     /locus_tag="VFZ86_03765"
     CDS             complement(465..1418)
                     /gene="mutM"
                     /locus_tag="VFZ86_03765"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEX5641440.1"
                     /translation="MPELPEVETVRRRLMTCLPGLLLREVVVNDWTVSVQTEHELNAY
                     LAGRRVTGLRRRGKYLLVDLGSPEEPAATPADGGAGPSVAVIHLRMTGQLLFRPEPGE
                     RPARFEWRLEPATTLFFQDVRRFGRLWALEPAAEEEFFASRGMGPEPFGPEFTVAYLR
                     GALGDRRAPLKAFLLDQRRIAGVGNIYADEALFRARLHPLRAAGSVGPREARRLHAAL
                     LETLQAGIDHEGSSIESFIDPAGRRGSFQEILNVYQRTGEPCRVCGATVERVVVGGRG
                     THYCPGCQPRRGGMSSGSRRRLGETVPPRSAKPADWEAPGR"
     gene            complement(1411..2862)
                     /locus_tag="VFZ86_03770"
     CDS             complement(1411..2862)
                     /locus_tag="VFZ86_03770"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX5641441.1"
                     /translation="MNVRYAVTPPTPDEVTRAALAALGPGEAGWLVGGCLRDELLGRR
                     VRDVDIVVDGRQEALARALADRFGGAVYATSDVFGGWRVVVGDLHIDVAAVRGGPPGD
                     PPDPATRALRLEADLRARDVTVDALARPLDGDSIVDPLSGLSDLAAGRLRLCSPASLD
                     DDPLRVLRLARLARGFDLVPDAAATEAAFRAAPGLAGVSGERVRDELCALLGTRAAPV
                     ALRDLAVWGALTVVLPEVDRLRGVEQNPYHHLDVFEHTLEALTYVVGVVAQLGGRRFL
                     ATPAEAGMPGADPIVPVAWAVLLHDIGKPVVRVVDDQDRVIFWHHDETGRQMCADIGR
                     RLSFSNRFVEYVGTLVRQHLRLGFLTREQPLTRRALARYRRDVSPWVFESVVVSLCDR
                     LATRGEKTSLASMARHYRLARAVWTAVSKAPAPQLLSGDDVMELLGIEPGPAVGQALD
                     ALEEEIEAGEVTDADGARAFLEEWWRRGETPDA"
     gene            complement(2869..4044)
                     /gene="alr"
                     /locus_tag="VFZ86_03775"
     CDS             complement(2869..4044)
                     /gene="alr"
                     /locus_tag="VFZ86_03775"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEX5641442.1"
                     /translation="MLAGRHRAVAEVDLEAIRHNVRRLMRDLPDGAVHCAVVKANGYG
                     HGAAPVARAALEAGSTWLGVATALEAEELRAYGFTAPILIFGPLTGTGLERAATSGAE
                     VVGWSEPFIAEAARLGAGVHVKYDTGMGRLGVPAPEVVRFCEQAHAGGVLSGLMSHFA
                     TADEEDASFFEQQLGRFTSLARDLKRQYPRLLCHTANSAATLRDPRAHFDMVRTGIAL
                     YGLAPANDDPYRHELRPAMKVVSYIAGVRDAAPGDSVGYGRTWRAAKRTRIGIVPVGY
                     ADGVRRALGNRGEVLVAGRRCPIVGRISMDQLTVKLPDDWGQAGDEVVLFGAAGDGVA
                     GSAGAWTEPDGPRLDGPETPRILCEEVARLLGTINYEVACDVAPRVVRRYRGVDPAA"
     gene            complement(4045..4521)
                     /locus_tag="VFZ86_03780"
     CDS             complement(4045..4521)
                     /locus_tag="VFZ86_03780"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX5641443.1"
                     /translation="MSTTDPPREGEALVRDIMTTPVVSVPSSMTVKDLAALFRDQRIG
                     GVPVVDDDRLVGIVTEGDLMALDADIQMPHYFELFDSIIYLGSQKKFKEQLEKASAAT
                     VGQLMTDEVKTVAPDDPARAAATLMSKHKFDRVPVVEGEAVVGIVTRHDIMKILGL"
     gene            complement(4508..6088)
                     /locus_tag="VFZ86_03785"
     CDS             complement(4508..6088)
                     /locus_tag="VFZ86_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEX5641444.1"
                     /translation="MDESLLMGLYTADGMQKVDGLAIDGIGIPGGHLMERAGLAVATE
                     ILERYAPEEAVVFAGKGNNGGDGFVVARELFDAGCEVTVFLLAAPAEYSGDAKLNLDI
                     LTRLGVDVRPATDDGGALAEDAMVLTEMADVVVDAVFGTGFTGAAKGPAAAVIELMNE
                     APGTVVSVDIMSGVGASTGAVHGPAVEADLTVTLHAAKVGHFVTPGGALSGEVVIAPI
                     GIPALCDQDPEVWLLTDEAMGELVLPKRSLDHKRSVGTVLVIGGSKGMEGAAHLAAFA
                     ALRGGAGLVHAALPDGAGAQKPFAEVITVTVPGVDGRFGMAARSALFEQADQLGAVAV
                     GPGLGRADEARLLVRELLGVDRPLLLDADGLYALGDKLELLAGRSRPTVITPHEGELG
                     RLIGRPAAEVAAQRLECARSAARRGDVTVVLKGEATIVADPSGRAYVVPTGNPGLATP
                     GTGDVLSGVIVAQLAKGLPATDAACLGAYLHGFAADLAAEEAVGTEAMVASDLFNSLP
                     AAVERLKAGPQEESHEHH"
     gene            complement(6081..6461)
                     /gene="acpS"
                     /locus_tag="VFZ86_03790"
     CDS             complement(6081..6461)
                     /gene="acpS"
                     /locus_tag="VFZ86_03790"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012526383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEX5641445.1"
                     /translation="MLLGLGIDVTEAARIRRLLERLPRAYERLFSEQERAYCDGFADP
                     WSRYAARFAAKEAVGKALGIGIIGFVWRDIEVLSGGKPLIALHGGVAGIARRLGVTRV
                     EVSLSHTGGMAYAVAAALKEDADG"
     gene            complement(6741..>7611)
                     /locus_tag="VFZ86_03795"
     CDS             complement(6741..>7611)
                     /locus_tag="VFZ86_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX5641446.1"
                     /translation="VPVQIDVASEFRYRQPVFDPDTLVIGITQSGETADTLAAMRLAR
                     QAGSPVLALTNIMGSQATRDADHVFFTRAGLEIGVAATKTHTAQVAALLVLALRLAWA
                     RGVQPEAELRRLGRELRAVPEKMEELLARPSAVHEIAGRYHAERFFLYLGRGMGYAVC
                     LEGALKLKEIAYIPTEAYAAGEMKHGPIALLDKESPVVVVADDSPVFPKLVSNIEEVR
                     VRGADVIAVATDGNEVIGELSKDVLWVPKTDPHLAPILTVIPLQLLAYHIAGIKGLNV
                     DQPRNLAKTVTVE"
BASE COUNT         1028 a         2806 c         2789 g          988 t
ORIGIN      
        1 gactgtcctg ggggccgagc gcagccggaa ggctcgcacc gctcctgtcg cgggcgcgcc
       61 ggcggcgtgt gccgcggccg tgaggtcgcg gcgcaggggc cggcgcgcga agcgcacgag
      121 gtgaccgtgg cagcggcagt cgccggcgcc gaagcggcgt ggccggcgct ccgcgccggg
      181 cagcgccgcc agggcgccgg cgagcgcaca ctcccccgtc gccccgtcga cgagccaggc
      241 gcgcccggcg gggaacgcgg tgaagaggta gtcggcgtgc catcgcagct gtttctcccg
      301 ggcgagatgg cgcgcgacgc gcgcctcccg cgcccgcgcg gcgctgccca cgtaggcgta
      361 ccagccgcgt tccagcgtga ccggcccgag cgatccgacc tgcagcgtcg agcgacgcgg
      421 cacgcgggtg aggacgatgt agaggcgcgg gtcgacctgc gccgtcaccg gcccggggct
      481 tcccagtcgg ccggcttggc ggagcgcggc gggaccgtct cccccagccg gcgccgcgac
      541 cctgacgaca tgccgccgcg gcgcggctgg cagccgggac agtagtgcgt gccgcgaccg
      601 ccgacgacga cgcgctcgac cgtcgccccg cacacccggc acggctcgcc ggtgcgctgg
      661 tacacgttca ggatctcctg gaagctgccg cgccgcccgg cgggatcgat gaagctctcg
      721 atgctggagc cctcgtggtc gatgccggcc tggagggtct cgaggagggc cgcgtgcagg
      781 cgccgcgcct cacgcggccc gacgctccca gctgcacgca gcggatgcag gcgggcgcgg
      841 aacaacgcct cgtcggcgta gatgttcccg acgccggcga tccgccgctg gtccagcagg
      901 aaggccttga gcggcgcgcg cctgtccccc agcgcgccgc gcaggtacgc gaccgtgaac
      961 tccggcccga acggctcggg gcccatgccg cgggaggcga agaactcctc ctcggccgcc
     1021 ggctcgagag cccacaggcg gccgaacctg cgcacgtcct gaaagaagag cgtggtggcc
     1081 ggctcgaggc gccactcgaa gcgagccggc cgctcgccgg gctccgggcg gaacaacagc
     1141 tgtccggtca tgcgcaggtg gatgacggcc acggacgggc cggcgccgcc gtcggccggc
     1201 gtggccgcgg gctcctccgg cgaccccaga tcgaccagca ggtacttgcc gcggcggcgc
     1261 agccccgtga ctcggcgccc tgcgaggtac gcgttcagct cgtgctcggt ctgcacgctc
     1321 acggtccagt cgttgacgac gacctcccgg agcagcaggc cgggcaagca ggtcatgagc
     1381 cgccgccgga cggtctcgac ctcggggagc tcaggcatcc ggtgtctcgc cgcgccgcca
     1441 ccactcctcc agaaaggcgc gggcgccgtc cgcgtcggtg acttctccgg cctcgatctc
     1501 ctcctcgagg gcgtccagcg cctggcccac agccggcccc ggctcgatgc cgagcagctc
     1561 catgacgtcg tcgccgctga gcagctgcgg cgccggcgcc ttgctcaccg ccgtccacac
     1621 ggcacgcgcg agccggtagt gccgcgccat cgaggcgagc gacgtcttct caccccgcgt
     1681 cgccagccgg tcgcagagcg acacgaccac ggactcgaac acccaagggc tcacgtcgcg
     1741 gcgataccgg gccagggcac ggcgcgtcag cggctgctcc cgcgtgagga acccgagccg
     1801 caggtgctgc cgtacgagcg tgccgacgta ctccacgaac cggttgctga agctgagacg
     1861 ccggccgatg tcggcgcaca tctggcggcc ggtctcgtcg tggtgccaga agatgacccg
     1921 gtcctgatcg tcgaccacgc gtaccaccgg cttgccgatg tcgtgcagga ggacggccca
     1981 cgcgacggga acgatcgggt cggcgccggg catgccggcc tccgccggcg tcgccaggaa
     2041 gcgccggccg cccagctgtg ccaccacacc gaccacgtac gtgagggcct cgagggtgtg
     2101 ctcgaagacg tccaggtggt gataggggtt ctgctccacg ccgcgcaggc ggtccacctc
     2161 ggggaggacc acggtgagcg cgccccacac ggcgaggtcg cggagcgcga ccggggcggc
     2221 gcgcgtgccg aggagcgcgc agagctcgtc gcggacacgc tcgccgctca cgcccgcgag
     2281 gccgggcgcc gcgcggaagg ccgcctcggt ggccgccgcg tccggcacca ggtcgaaccc
     2341 gcgcgccagg cgcgccagcc gcagcacgcg cagcggatcg tcgtcgagcg aggccggtga
     2401 gcagagccgc agtcgtcccg cggcgaggtc ggaaaggccg ctgagcggat cgacgatgct
     2461 gtcgccgtcg agcgggcgcg cgagcgcatc cacggtgaca tcgcgcgccc ggaggtccgc
     2521 ctccaggcgg agagcgcgcg tcgccgggtc cggcgggtcg ccgggcggcc cgccgcgcac
     2581 ggcggcgacg tcgatgtgca gatcgccgac gacgacccgc cagccgccga agacgtccga
     2641 cgtcgcgtac acggcgccgc cgaagcggtc ggccagggcg cgcgccagcg cctcctgccg
     2701 tccgtcgacg acgatgtcga cgtcacgaac gcgtcgcccg aggagctcgt cccgcaggca
     2761 gccgcccacc agccatccgg cttcgccggg gcccagtgcg gcgagtgcgg cccgggtgac
     2821 ctcatcgggc gtcggcggcg tgacggcgta ccggacgttc acggtgcctc aggccgcggg
     2881 atccacgccg cggtagcggc gcaccacccg cggcgccacg tcgcaggcga cttcgtagtt
     2941 gatggtgccg agcaggcgcg ccacctcctc gcagaggatg cgcggcgtct cggggccgtc
     3001 cagacggggc ccatcgggct ccgtccacgc gccggcggag ccggccacac cgtcgccggc
     3061 ggcgccgaac agcacgacct cgtcgcccgc ctggccccag tcgtcgggca gcttgaccgt
     3121 gagctggtcc atgctgatcc tgccgacgat cgggcaacgc ctgccggcga ccagcacctc
     3181 gccgcggttg ccgagcgcac ggcgcacgcc gtccgcgtag ccgacgggga cgatgccgat
     3241 gcgcgtgcgc ttcgcggcgc gccacgtgcg cccgtacccc accgaatcac cgggcgcggc
     3301 gtcgcggacg ccggcgatgt acgacacgac cttcatcgcc gggcgcagct catgcctgta
     3361 cgggtcgtcg ttggcgggcg ccagcccgta cagcgcgatg ccggtgcgca ccatgtcgaa
     3421 gtgggcgcgc gggtcccgca gggtcgccgc gctgttggcc gtgtggcaca gcagacgagg
     3481 gtactgccgc ttgaggtccc gggccagact ggtgaaccgg ccgagctgct gctcgaagaa
     3541 gctcgcgtcc tcctcgtccg ccgtggcgaa gtggctcatc agaccggaca gcacacctcc
     3601 cgcgtgcgcc tgctcgcaga accggaccac ctcgggcgcc ggcacgccga gccgccccat
     3661 gcccgtgtcg tacttgacgt ggacgccggc gccgaggcgg gccgcctcgg cgatgaacgg
     3721 ctctgaccac ccgacgacct ccgcccccga ggtcgcagcc ctctcgaggc cggtccccgt
     3781 gagcgggccg aagatgagga tgggcgcggt gaagccgtac gcccgcaatt cctcggcctc
     3841 gagcgccgtc gccacgccga gccaggtgga gccggcctcg agcgcggcgc gcgccacggg
     3901 cgcggcgccg tggccgtagc cgttggcctt gaccacggcg cagtgcacgg cgccgtccgg
     3961 caggtcgcgc atgagccggc ggacgttgtg gcggatcgcc tcgaggtcga cctcggcgac
     4021 ggcgcggtgt cgtccggcca gcactcagag tcccaggatc ttcatgatgt cgtgtcgggt
     4081 gacgatgccg acgaccgcct caccttcgac caccggcacc cggtcgaact tgtgcttcga
     4141 catcagcgtg gcggcggcgc gggccgggtc gtccggagcc accgtcttca cctcgtcggt
     4201 catgagctgc cccacggtcg ccgcagaggc cttctcgagc tgctccttga acttcttctg
     4261 gctaccgaga tagatgatgc tgtcgaagag ctcgaagtag tgcggcatct ggatgtcggc
     4321 gtccagcgcc atgaggtcgc cctcggtgac gatgccgacg agtctgtcgt cgtcgaccac
     4381 gggcacgccg ccgatccgct ggtcacggaa aagggcggcg aggtccttga ccgtcatgct
     4441 cgacggcacc gagacgacgg gcgtggtcat gatgtcgcgg acgagcgctt cgccctcacg
     4501 cggggggtca gtggtgctca tggctctcct cctgtggacc ggccttgagc cgctccacgg
     4561 cggcgggcag ggagttgaac aggtcgctcg ccaccatggc ctctgtgccc actgcctcct
     4621 cggccgccag gtcggcggcg aagccgtgga ggtacgcgcc gagacaagcg gcgtccgtgg
     4681 ccggcaggcc cttcgcgagc tgggcgacga tgacgccgct gagcacgtcg ccggtgcccg
     4741 gcgtggcgag gccggggttg ccggtcggga ccacgtaggc ccgtcccgac gggtcggcga
     4801 cgatggtcgc ttcgcccttg aggacgacgg tcacgtcgcc gcgtctggcg gcggagcggg
     4861 cgcactcgag gcgctgggcc gcgacctcgg ccgccgggcg gccgatcagg cggcccagct
     4921 caccctcgtg cggcgtgatc accgtgggcc ggctgcgtcc ggcgaggagc tcgagcttgt
     4981 ctccgagcgc gtagaggccg tcggcgtcga gcagcagcgg ccggtccacg ccgagcagct
     5041 cgcgcaccag cagccgcgcc tcgtcggcgc ggccgaggcc gggaccgacg gcgacggccc
     5101 cgagctggtc cgcctgctcg aagagcgccg agcgggcggc catgccgaac cggccgtcga
     5161 cgccggggac ggtcacggtg atgacctcgg cgaacggctt ctgcgcgccg gcgccatcgg
     5221 gcagcgcggc gtgcaccagg ccggcgccgc ccctgagggc cgcgaacgcg gccaggtggg
     5281 cggcgccctc catgcctttg gaccccccga tgacgaggac cgtgccgacc gagcgcttgt
     5341 ggtcgagaga gcgcttgggg agcaccagct cgcccatcgc ctcatcggtg agcagccaca
     5401 cctccgggtc ctggtcgcag agcgccggga tgccgatcgg cgcgatcacg acctcgcccg
     5461 agagcgcccc cccgggcgtg acgaagtggc cgaccttggc tgcgtgcagc gtgacggtga
     5521 ggtcggcctc gacggccggc ccgtgcacag cgccggtgct cgcgcccacg ccgctcatga
     5581 tgtcgacgct caccacggtg cccggcgctt cgttcatgag ctcgatgacg gcggcggccg
     5641 gccccttggc cgcgccggtg aagccggtgc cgaagacggc gtccacgacg acgtcggcca
     5701 tctccgtgag caccatcgcg tcttcggcga gcgccccgcc gtcatcggtc gcggggcgca
     5761 cgtccacccc gaggcgggtg aggatgtcca ggttcagctt cgcgtcgccc gagtactcgg
     5821 ccggcgccgc gagcaggaac acggtgacct cgcagcccgc gtcgaacagc tcgcgcgcga
     5881 ccacgaaccc atccccgccg ttgttgccct tgccggcgaa gaccacggcc tcctcgggcg
     5941 cgtaccgctc gaggatctcg gtcgccaccg cgagcccggc gcgctccatc aggtgcccgc
     6001 cggggatgcc gatgccgtcg atcgccagcc cgtcgacctt ctgcatgccg tcggcggtgt
     6061 acagccccat cagcagggac tcatccatcc gcgtcctcct tgagtgcggc ggccacggcg
     6121 tacgccatgc cgccggtgtg gctcagcgag acctcgaccc tcgtcacgcc caggcgccgc
     6181 gcgatgccgg cgacgccgcc gtgcagcgcg atgaggggct taccacccga gagtacctcg
     6241 atgtcgcgcc acacgaaccc gatgataccg attccgagcg ccttgccgac cgcctccttg
     6301 gcggcgaagc gggcggcgta gcgcgaccag ggatcggcga agccgtcgca gtacgcgcgc
     6361 tcctgctcgc tgaacagacg ctcgtaggcg cgcggcagcc gctcgagcag cctgcggatg
     6421 cgcgcggcct cggtcacgtc gatgccgaga ccgagcagca gtccgccggg cgggacgacg
     6481 aaggtcccgg gaaaccccga gaacctcccc gcctcgtccg ccggggtctg cccggcgcgc
     6541 tggtgcctgt cactcatgcc ggcattctat ccgccgggtc gccggcgctc cccccgcacc
     6601 ggcgctcgct ccggggcctc acgtggccgc cgcacccgga tccctgcgcc ggagagcgcc
     6661 gtggagcgca tcggcccttg acgtcaccgg tggcgtcgtg gatggcgtca ccgcgggacg
     6721 caccatgccc gggcctcgcc ctattcgacc gtcacggtct tggccaggtt gcgcggctgg
     6781 tcgacgttca gccccttgat gccggcgatg tggtaggcca gaagctgcag cgggatcacc
     6841 gtgaggatcg gcgccagatg cgggtcggtc ttcggcaccc acaggacgtc cttggagagc
     6901 tcgccgatga cctcgttgcc gtcggtcgcc acggcgatga cgtcggcgcc gcgcacccgg
     6961 acctcctcga tgttgctcac gagcttcggg aagaccgggc tgtcgtcggc gaccacgacc
     7021 accggcgact ccttgtcgag gagagcgatg gggccgtgct tcatctcgcc ggcggcgtac
     7081 gcctcggtgg ggatgtaggc gatctccttg agcttgagcg cgccctcgag gcagacggcg
     7141 tagcccatgc cgcgaccgag gtacaggaag aagcgctcgg cgtggtagcg cccggcgatc
     7201 tcgtggacgg cgctggggcg ggcgagcagc tcctccatct tctcggggac ggcgcgcagc
     7261 tcgcgcccga ggcggcgaag ctcggcctcg ggctgcacgc cgcgcgccca cgcgaggcgc
     7321 agggcgagca cgagcagggc ggcgacctgg gcggtgtgcg tcttggtcgc cgccacgccg
     7381 atctcgagtc cggcgcgcgt gaagaacacg tggtcggcgt cgcgcgtcgc ctggctgccc
     7441 atgatgttgg tgagggcgag gacgggcgag ccggcctgac gcgcgagccg catcgcggcg
     7501 agcgtgtcgg cggtctcgcc cgactgcgtg atgccgatca ccagggtgtc ggggtcgaag
     7561 acgggctggc ggtagcggaa ctcgctggcc acgtcgatct gcaccgggac g
//
LOCUS       DASUMX010000080        10527 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1459829, whole genome shotgun sequence.
ACCESSION   DASUMX010000080 DASUMX010000000
VERSION     DASUMX010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 10527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10527
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1459829"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            518..862
                     /locus_tag="VFZ86_03800"
     CDS             518..862
                     /locus_tag="VFZ86_03800"
                     /inference="COORDINATES: protein motif:HMM:NF036294.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HEX5641447.1"
                     /translation="MPRMDGTGPMGQGPMTGGRRGRCASDTAEIPTAAGPGMGRGMGY
                     GRGMGAGRGRGGRGYRNQYYATGLTGWQRAEMAAAPAGETVGDAARVERLEAKLTEAL
                     DGLSRLEGTERT"
     gene            1072..1290
                     /locus_tag="VFZ86_03805"
     CDS             1072..1290
                     /locus_tag="VFZ86_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010877862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX5641448.1"
                     /translation="MTGAAPAAVAAAPLVARLDDPDLCIGCGICVDACPRSAIELDDV
                     VSIDRALCTGCGECVDACPRGVLSLVEA"
     gene            1293..2168
                     /locus_tag="VFZ86_03810"
     CDS             1293..2168
                     /locus_tag="VFZ86_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006963874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5641449.1"
                     /translation="MRIAIASGKGGTGKTTLAVNLATLLADEGVKVRLLDADVEEPDC
                     HLFLHPSAVTSTPVSVPLPVVDEARCTACRVCAEACAFKAITLVGSTVLVFPELCHSC
                     GACSLLCPESAIREEGRDTGSVETGTVSTPSGHFTLVTGLLNVGEAKAVPVTKATLAT
                     AGGADVVLIDAPPGTSCPVIESVRGSDLVVLVTEPTPFGLNDLRLAVEMVRALGLPAV
                     VAVNRMGIGDDRVQRYCAEESLDVVLELPNDRRIAEAYARGELLVDALPDLRAELRAA
                     WKRIAVAAESAEVTA"
     gene            2165..3088
                     /locus_tag="VFZ86_03815"
     CDS             2165..3088
                     /locus_tag="VFZ86_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5641450.1"
                     /translation="MTEPRELVVISGKGGTGKTSVVAAFAALAGGAVLADCDVDAADL
                     YLVLDPDVGPPHDFSGRQQAIIDPAACTACGRCAEVCRFAAVLGGGADGYTVDPIACE
                     GCGLCLRVCPRDAVGMGPVINGAWMLSETRFGPLVHARLGVAEDNSGKLVTLVRKEAR
                     ETALRRGLPLVIVDGSPGIGCPVIASLAGADLVLAVTEPTVSGRHDLERVLQVVRQFR
                     LPFAVIVNKADLNAPLAATIAAETTAQGALFVTTIDYDPSVTRAQVEGVDVITYGGRA
                     AAATTEAWEAVREAFGRELVSVPATEERVES"
     gene            3085..4044
                     /locus_tag="VFZ86_03820"
     CDS             3085..4044
                     /locus_tag="VFZ86_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005024301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX5641451.1"
                     /translation="MNESLDTRSLVYGPVPSRRLGRSLGVDLVPFKTCTYDCVYCQLG
                     RTTHKTVRRERWVAADAVVDQVKRQRGSNPDVIALAGSGEPTLYAGLGEVVDGVKAIT
                     DVPVAVITNGSLLGLPEVRRDLAHADIVLPSLDAADEETHQSINRPHDSLDLKGLVDG
                     MAGFREAFAGQIWLEVMLLDGITGTDEHVESLAALAACIAPDRIQLNTVVRPPAESAA
                     RPVAPKRLAAFASVFKPRAEVIAGAPALQPDQEATAADVFTLIARRPCTVADVAAGLG
                     IHQNAALKALTGLVERSVAVRRDHEGGVFYAAADNARSDAEEP"
     gene            4041..4406
                     /locus_tag="VFZ86_03825"
     CDS             4041..4406
                     /locus_tag="VFZ86_03825"
                     /inference="COORDINATES: protein motif:HMM:NF014620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HEX5641452.1"
                     /translation="MKVAVSAQSNDIDSLVDPRFGRAAWFVIVESESLEWTAVDNREN
                     ADASGGAGVQAGTIVADQGVQSVITGNVGPNAHKVLAAAGVDIYQAGNGITVRDAITG
                     LGRNELSRLSAPTVAGHWS"
     gene            4436..4579
                     /locus_tag="VFZ86_03830"
     CDS             4436..4579
                     /locus_tag="VFZ86_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641453.1"
                     /translation="MPADWPDNDLIGDRAIVPPAQTVADARKRPQKYGCYDWWFCHKP
                     LEE"
     gene            4736..5026
                     /locus_tag="VFZ86_03835"
     CDS             4736..5026
                     /locus_tag="VFZ86_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641454.1"
                     /translation="MRAVIAAGTYVAQDMRDREGLTRPMLRRAALRMAVSRQPALRRL
                     GSAYLRNDPPAPEELPGHASPALFAGVRPLPLPASPERPVIDVKPAAGREAI"
     gene            5083..5634
                     /locus_tag="VFZ86_03840"
     CDS             5083..5634
                     /locus_tag="VFZ86_03840"
                     /inference="COORDINATES: protein motif:HMM:NF014101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HEX5641455.1"
                     /translation="MPRPPIERAVSGVPRVTLFKPAGVPTRELEQLQLGVDELEAIRL
                     VDLEGLSHEQAAEVMGVSRQTVGRVLERGRGRVAEALVGGKAILIGGGQYRVEPRRMR
                     CFACQARWVEQGEDAATVCPECGARDVGICWGAGERCGDGRGRRGGGPGRGAGKGHGP
                     GCDKGHGPGCGQGRGPHGRGATT"
     gene            5672..6052
                     /locus_tag="VFZ86_03845"
     CDS             5672..6052
                     /locus_tag="VFZ86_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014322961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HEX5641456.1"
                     /translation="MSDQSKSVRIAIPSELPGGLEARRSGHFGRCECFTLVDIADGSV
                     NEVQVLTNAPHTEGGCMAPVMALAEHMVDAIVVDGIGGRPLMGFNQVGIAVHAGVGGD
                     VQTTVGAYLEGGLPVVGLEGACQH"
     gene            complement(6067..6648)
                     /locus_tag="VFZ86_03850"
     CDS             complement(6067..6648)
                     /locus_tag="VFZ86_03850"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5641457.1"
                     /translation="MADTFDPMNASRLEDPSRLEALPQAAVVGLLRLDGAETVVDYGA
                     GTGIYTLAVAAAVPRGRVFAIEALPRLAQLFREKITPELAARICLVETGDNVVPLDDA
                     EADRVVMVDVLHHLHDQPEALEEVVRVLRPGGLFVVLDWGDKERPVGPPPGHVLGLEA
                     VRGIIAGMGLEVVEAHEPGELLPYHVVVVAAKP"
     gene            complement(6648..7052)
                     /locus_tag="VFZ86_03855"
     CDS             complement(6648..7052)
                     /locus_tag="VFZ86_03855"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006966714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX5641458.1"
                     /translation="MDMKVYFPGGKRVYADYGNFTIETDQPARGGGDDSAPAPFDLFL
                     ASIGTCAGIFALGFMQQRGIDPAGSSLQMTPQFDPNVGLISRIDLQLTLPDGFPEKYQ
                     AAIINAMNLCSVKKHLHQPPEFQITTTAAIPS"
     gene            7148..7474
                     /locus_tag="VFZ86_03860"
     CDS             7148..7474
                     /locus_tag="VFZ86_03860"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX5641459.1"
                     /translation="MTDDRQRYAIHAAVFAALANPTRHELMHHLCDSPRTPSELADLL
                     EVSRPNVSQHLAILQRDGLVRRHREEGHVLWEVVNPRLAEACALIDEILGPELALRAH
                     ALTHER"
     gene            7490..7687
                     /locus_tag="VFZ86_03865"
     CDS             7490..7687
                     /locus_tag="VFZ86_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX5641460.1"
                     /translation="MIAVNVHACPQNHPCPAVHHCPEGAIIQDDIYSAPRIDEDKCTE
                     CGVCTSVCRVFSMVRDEVTVG"
     gene            7687..7887
                     /locus_tag="VFZ86_03870"
     CDS             7687..7887
                     /locus_tag="VFZ86_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HEX5641461.1"
                     /translation="MAHFDLICKECGHRFELVTGGAIREKQKRCKECGSENIRQSFLS
                     YLQNGPLSSPTCGEQRPSSGYG"
     gene            7987..8547
                     /locus_tag="VFZ86_03875"
     CDS             7987..8547
                     /locus_tag="VFZ86_03875"
                     /inference="COORDINATES: protein motif:HMM:NF017778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase family protein"
                     /protein_id="HEX5641462.1"
                     /translation="MDVPPRDLSLDEFIVEMSGQPVKALTHERLMHLAGRLRLSHALV
                     GASVAFSRDGYARNLVCRTSSFELLVLCWRPGQESTIHDHAGALNAIRVYSGELTSRT
                     FTRTSGAAAGDGPVAQDGEERVPADGHMVGLDRNGIHQLANTSGQDLVTVHVYAPALM
                     ELNVYSTTSAEVERRPLRYTLADDLA"
     gene            complement(8597..8929)
                     /locus_tag="VFZ86_03880"
     CDS             complement(8597..8929)
                     /locus_tag="VFZ86_03880"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX5641463.1"
                     /translation="MDESLEREVILLHANVCQGLSDPKRILMLYALRDQPLHVHELAE
                     ALHVPQPTVSRHLKVLRERHLVEARREGPSVLYSLADERVIDALDILRSVLLGTLSRQ
                     GLLAETMD"
     gene            complement(9102..9803)
                     /locus_tag="VFZ86_03885"
     CDS             complement(9102..9803)
                     /locus_tag="VFZ86_03885"
                     /inference="COORDINATES: protein motif:HMM:NF037015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX5641464.1"
                     /translation="MRKTHLMVLAIVLAVAVLAVGAVGAYAGLADDDNPPVTTTDVPA
                     AGAQYWDAATIVATATDDEGIAYIYHEFDNGLVRLATIADKPLSAAISIPTDKDMPLA
                     AGAHKVKVWAQDINGNVEAQQTVTFEIVKDTVKPTTAARFAVVLKGRVATLKYRVTDP
                     EPTRGTATVMIKVKNSAGKVVKTMSAGTVTVNVPLAKNFRCGLARGTYRYYVYATDAS
                     GNAQSKVGSARLTVR"
     gene            complement(9874..>10527)
                     /locus_tag="VFZ86_03890"
     CDS             complement(9874..>10527)
                     /locus_tag="VFZ86_03890"
                     /inference="COORDINATES: protein motif:HMM:NF021252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family (seleno)protein"
                     /protein_id="HEX5641465.1"
                     /translation="AVWYSCVEALAAIPGVNQADWASLPMNMFRFGGGGIAGWGTICG
                     TLNGAAAFTSMAVGTNVVDGKPGWTHRNNLINGIFQYYAETPLPTNNTFKSSQGALGL
                     PAWTPPAVDGYTMIENAPTSTANSPLCHSSLVQWTTTTGASDGGPLQRDRCGKACFDV
                     ARKTIEVLNKYFELAEAPPVGLDPSVAACGACHKTYTGAKMACGSCHDNTTDDGHMN"
BASE COUNT         1611 a         3655 c         3661 g         1600 t
ORIGIN      
        1 gccgagcacc tctttcggca gcgccgaggt cttgcaggcg accgcgtcga ccgcgaagcc
       61 gttggcctcg aacacccgct tgaccaccgc ggcctcgtcc tgcagcccga tgcaatgcgc
      121 gacgcccagg cgcgcgaatc cgcagcggcg cgcgaagtcc atgatctcct cgacacgcgt
      181 caggcggcag tagccgccgg cctcgacgcg agcggcctgc cgggcaagct cgccgtcggc
      241 ctcgtcccgg tacgcctcga tcagctcatc cctgtccaga tcgcgggtcg gacagttctt
      301 ggggaacggg cccgtttcag cctcacaaca tgcaacgcta cgacagtcgg aacatgcgta
      361 catctttctc gcccctctct cggctgatgc tccattttac cgggatcgag cgtcccgcag
      421 ggcagcttgc ttttctccgg cacgggtata atatgggcat atgctcatta ctaccagccg
      481 gtggcggcct gatccgccac gctggagagg aggcgtcatg ccacgcatgg acggaacagg
      541 gccgatgggg cagggcccca tgaccggtgg gcgccgcgga cgctgcgcca gcgacactgc
      601 cgagattccc actgccgccg gacccgggat gggtcgtggc atgggctacg gccgcggcat
      661 gggcgccggc cgcggacgcg gtggtcgcgg ctaccgcaat cagtactacg cgaccgggct
      721 cacaggctgg cagcgcgccg agatggccgc cgctccggcc ggtgagacgg tcggcgatgc
      781 cgcgcgtgtc gagcgcctcg aggcgaagct cactgaggcg ctcgacgggc tctcgcggct
      841 ggaagggact gagcgaacgt gacttcccgc ggctcaggca agggcgcgcc ggacggtcgc
      901 cgtgtcggca atgtcgatct tggcggcagt cgaggcaagg gcggcgccgg ccgtggtcag
      961 cggggcggcg gcggagtgcg ccgccgcctg cgtgacggca gcggcgccgg caggccgatc
     1021 gcaggcgcgc ccgaaagccg gacggcgggc ggcgaggcga cgggcggcgt cttgacgggc
     1081 gccgcgccgg cggccgtcgc cgcggcgccc ctcgtcgcac ggctcgacga ccccgatctc
     1141 tgcatcggct gtggcatctg cgtggatgcg tgtccccggt cggcgatcga gctggacgac
     1201 gtcgtgtcga tcgaccgcgc gctctgcaca ggctgcggcg agtgcgtgga cgcgtgtccg
     1261 cgcggcgtcc tctcgctcgt cgaggcgtga cgatgcgcat cgccatcgcc agcggcaagg
     1321 gcggtaccgg caagaccacg ctggccgtca acctcgcgac cctgctggcc gacgaagggg
     1381 tcaaggtccg gctcctcgac gccgacgtcg aggagccgga ctgccacctc ttcctgcatc
     1441 cgagcgccgt gacctccacg ccggtgagcg tgcccctgcc cgtcgtggac gaggcacggt
     1501 gcaccgcctg ccgcgtctgc gccgaagcgt gcgcgttcaa ggcgatcacc ctcgtcggct
     1561 ccacggtgct cgtgttcccc gagttgtgcc actcctgcgg cgcctgcagc cttctgtgtc
     1621 ccgagtccgc catccgcgag gaggggcgtg acaccgggtc ggtagagacc ggcaccgtct
     1681 ccactcccag cggccacttc accctggtga ccggactgct caatgtcggt gaggccaagg
     1741 cggtcccggt caccaaagcg accttggcca ctgcgggcgg cgccgacgtc gtgctcatcg
     1801 acgccccgcc gggcacgtcc tgcccggtga tcgagtccgt gcgcgggagc gacctcgtcg
     1861 tcctcgtcac ggagcccact ccctttgggc tcaacgacct gcgcctcgcc gtggagatgg
     1921 tccgcgcgct gggcctgccc gccgtggtcg cggtcaaccg gatggggatc ggcgacgatc
     1981 gcgtgcagag gtactgcgcc gaggagtcgc tcgacgtcgt gctcgaactt cccaacgacc
     2041 gccgcatcgc cgaggcgtac gcacgcggtg agctgctcgt cgacgcgctc cccgatctac
     2101 gcgcggagct gcgcgcggcg tggaagcgca tcgccgtcgc cgcggaatcg gcggaggtga
     2161 cggcatgacg gagccccgag aactggtcgt gatcagcggc aagggcggca ccggcaagac
     2221 cagtgtggtc gccgcgttcg cagcgctcgc cggcggcgcc gtgctcgccg actgcgacgt
     2281 cgacgccgcc gacctgtacc tggtgctgga cccggacgtc ggaccgccgc acgacttcag
     2341 cggccggcag caggcgatca tcgatcctgc cgcctgcaca gcctgcggcc ggtgcgcaga
     2401 ggtgtgccgc ttcgcggcgg tcctcggcgg cggggcagac ggctacacgg tggacccgat
     2461 cgcctgcgaa ggctgcgggc tctgcctccg ggtctgcccc cgcgacgccg taggcatggg
     2521 ccccgtgatc aacggcgcct ggatgctctc ggagacccgc ttcggcccgc tcgtgcacgc
     2581 ccgcctcggt gtggccgagg acaactcggg caagctcgtc acgctcgtgc gtaaggaggc
     2641 gcgagagacc gccctgaggc gcggactgcc gctggtgatc gtggacggct cgcctggcat
     2701 cggctgcccc gtgatcgcct cgctcgccgg agccgacctg gtgctcgcgg tgacggagcc
     2761 gacggtgtcg ggccggcacg acctcgagag agtactgcag gtcgtgcgtc agttccgcct
     2821 gcccttcgcc gtcatcgtga acaaggccga cctcaacgcg ccgctcgcgg cgacgatcgc
     2881 cgcggagacg acggcgcaag gggcgctctt cgtcaccacc atcgactacg acccttcggt
     2941 gacgcgggcc caggtggagg gcgtcgatgt catcacctac ggcggccgtg ccgcagccgc
     3001 gaccactgag gcttgggaag ccgtccggga ggctttcggc cgcgagctcg tgtccgtccc
     3061 ggcgacggaa gagcgggtcg agtcatgaac gagagcctgg acacccgctc gctcgtctac
     3121 ggtccggtac cctcacgtcg cctcggacgc tcgctcggcg tggacctcgt gccgttcaag
     3181 acctgcacct atgactgcgt ctactgccag ctcggacgca cgacgcacaa gacggtgcga
     3241 cgtgagcgct gggtcgccgc cgacgcggtc gtggatcaag tcaaacgcca gcgggggtcg
     3301 aacccggacg tgatcgcgct ggccggatcg ggcgagccga cactctacgc aggcctcggc
     3361 gaggtcgtgg acggcgtcaa ggccatcacg gacgtccccg tggcggtcat cacgaacggc
     3421 tcgctgctgg gcctcccgga ggtccggcgc gacctcgcgc acgccgacat cgtgctgccg
     3481 tcactcgatg ccgccgacga agaaacgcac cagagcatca accgccccca cgacagcttg
     3541 gatctcaagg gactcgtgga cggcatggcc ggcttccgcg aggcgttcgc cggccagatc
     3601 tggctggagg tcatgctgct ggacgggatc accggcacgg atgagcacgt cgagagcctg
     3661 gcggcacttg cagcatgcat cgcgccggat cgcatccagc tcaacaccgt cgtgcggcct
     3721 ccggcggaat cggccgcgcg gccggtcgcc ccgaagcgcc tcgcggcgtt cgcatccgtc
     3781 ttcaagcccc gcgcggaggt catcgccggc gcgccggcgc tgcagccgga ccaggaggcg
     3841 acggccgccg acgtgttcac gctcatcgcg cggcggccgt gcaccgtcgc cgacgtcgcg
     3901 gcgggactcg gcatccatca gaacgcggcg ctcaaagcgc tcactgggct ggtagagcgc
     3961 agtgtggccg tgcgccgcga ccacgaaggc ggcgtgttct acgccgcggc agacaacgct
     4021 cgatccgatg cggaggagcc atgaaggtag cagtcagcgc tcagagcaac gacatcgatt
     4081 ccctcgtcga cccgcggttc ggccgcgcgg cctggttcgt catcgtcgaa tccgagagcc
     4141 tggagtggac ggcggtcgac aaccgcgaga atgccgacgc cagcggaggc gccggcgtcc
     4201 aggccggcac gatcgtcgcc gaccagggcg tgcagagcgt gatcaccggc aacgtcggtc
     4261 cgaacgcgca caaggtcctg gccgcggccg gcgtggacat ctaccaggcg ggcaacggta
     4321 tcacggtgcg tgacgccatc accggcttgg gcaggaacga gctgtcgcgg ctgagcgcgc
     4381 cgaccgtcgc cggccattgg tcgtgaccct cgtgcgaccc gtcgcagcag aacctatgcc
     4441 tgcggactgg cccgacaacg atctgatcgg cgatcgcgcc atcgttccgc cggcacagac
     4501 ggtggcggat gcgcgcaagc ggccccagaa gtatggctgc tacgactggt ggttctgcca
     4561 taagccactc gaggagtgat agcgaatgga gaccagagga atggagccgc gccgtcacag
     4621 gcggcgcagt ccctccgcct gtgacgggag aggaacaggc cggggaaggt acctcggcgg
     4681 agggcgcggc gccgggcggt cgagggatga ctcgagcctc acgacgcgca cggccatgcg
     4741 cgccgtgatc gcagccggga cgtacgtggc gcaagacatg cgagaccgcg agggtctgac
     4801 gcggccgatg ctgcgccgtg cggcgctgcg gatggcggtg agccggcagc cggcgcttcg
     4861 caggctcggc tccgcctacc ttcgcaacga cccgccggcg ccggaggaac tcccggggca
     4921 cgcctctcca gcgctgttcg ccggcgtgag gccgctgccg ctgccggcat ctcctgagag
     4981 gcccgtcatc gacgtcaaac cggcggccgg cagagaggcg atttgatggc cgggcgggcg
     5041 caggttatcc tgagcatatg ctaagaaccg acgaggagag ccatgccacg cccgcccatt
     5101 gagcgcgccg tctcgggcgt tccgcgcgtc acgctgttca agccggccgg agtgcccacc
     5161 cgtgagctcg agcagctgca gctcggcgtc gacgagctcg aggcgatccg cctggtagac
     5221 ctcgagggcc tgagccatga acaggccgcg gaggtcatgg gagtatcccg ccagacggtc
     5281 gggcgggtac tcgagcgcgg ccggggccgt gtcgccgagg ccctcgtggg cggcaaggcc
     5341 atcctcatcg gcggcggcca gtaccgcgtc gagccgcgcc gcatgcgctg cttcgcctgc
     5401 caggcccgat gggtcgagca gggcgaggac gccgcgaccg tgtgcccgga gtgcggagcc
     5461 cgggatgtcg gcatctgctg gggcgccggc gagcgctgcg gcgatggccg tggccgccgc
     5521 ggcggtggtc ccggccgcgg agccggcaag gggcacgggc cgggatgcga caagggccac
     5581 gggccgggat gcggtcaagg ccgcggtccg cacggccgcg gcgcgacgac ctgacctcat
     5641 cgaccacgat cacgaacgga gcgatatcac catgagcgac cagagcaaga gcgtccgcat
     5701 cgccattcca tccgaactcc ccgggggtct cgaggcccgc cgctccggcc acttcggtcg
     5761 ctgcgagtgc ttcaccctcg tcgacatcgc cgacggctcc gtcaacgagg tacaggtact
     5821 caccaacgcg ccgcacaccg agggtggctg catggctccg gtcatggcgc tggccgagca
     5881 catggtcgac gccatcgtcg tcgacggcat cggcggaagg ccgctcatgg gcttcaacca
     5941 ggtgggcatc gccgtccacg cgggagtcgg cggcgacgtg cagaccacag tgggtgcgta
     6001 tctggagggc ggcctgccgg tcgtcggcct cgaaggcgcc tgccagcact gatctgccgc
     6061 gcgggcctaa ggcttcgccg cgaccaccac gacgtgatac ggcagcagct cgcccggctc
     6121 gtgcgcctcg accacctcga ggcccatccc ggcgatgatg ccgcgcactg cttcgaggcc
     6181 gagcacgtgg ccgggcggcg gcccgaccgg acgctccttg tcgccccagt cgagcaccac
     6241 gaacagcccg ccggggcgca ggacgcgcac gacctcctcc agcgcctccg gctggtcgtg
     6301 gagatggtgg aggacatcga ccatgacgac cctgtccgcc tcggcgtcgt cgagcgggac
     6361 cacgttgtcg ccggtctcga cgaggcagat ccgcgcggcg agctccggcg tgatcttctc
     6421 gcggaacagc tgcgcgagcc gaggcagcgc ctcgatcgcg aacacacgcc ctctcgggac
     6481 ggccgccgcc acggcgagcg tgtagatgcc cgtcccggcg ccgtagtcga ccacggtctc
     6541 ggcgccgtcc agccgtagca ggccgaccac ggcagcctgc ggaagcgcct cgagtcgcga
     6601 gggatcctcg agccgcgagg cgttcatggg atcgaacgtg tcggccatca ggatgggatc
     6661 gcggccgtcg tcgtgatctg gaactcgggc ggctggtgca gatgcttctt cacgctgcac
     6721 aggttcatgg cgttgatgat cgccgcctga tacttctcgg ggaagccgtc aggcagcgtc
     6781 aactgaaggt cgatccggct gatcagcccg acgttcgggt cgaactgcgg cgtcatctgg
     6841 aggctcgagc cggcggggtc gatgccgcgc tgctgcatga acccgagcgc gaagatgccc
     6901 gcgcacgtgc cgatcgaggc caggaagagg tcgaagggcg ccggcgcgga gtcgtcgccg
     6961 ccgccgcggg ccggctggtc cgtctctatg gtgaagttgc cgtagtccgc gtacacgcgc
     7021 ttgccgcccg ggaagtagac cttcatgtcc atggtggatc ctttcgcacg gcccggaccg
     7081 cccgggcgct tctctgtgtg aacagttgcc atgttgcgaa atttcgaaac ttggcaataa
     7141 tgaagacatg accgacgacc gacagcgcta cgccatccat gccgcggtgt tcgccgccct
     7201 ggccaaccct actcggcacg agctcatgca tcacctctgt gactcccccc gcacgccgag
     7261 cgagctcgcc gacctgctcg aggtcagccg gccgaacgtc tcgcagcacc tcgccatcct
     7321 ccagcgcgac ggcctcgtac gccgccatcg cgaggaaggc cacgtgctgt gggaggtcgt
     7381 gaacccacgg ctcgccgagg cctgcgcgct catcgacgag atcctcggtc cagagctggc
     7441 cctgcgcgca cacgcactga cgcacgagag gtaaaaggag aaaccacgca tgatcgccgt
     7501 caacgtccac gcctgtcccc agaaccaccc ctgccccgcc gtgcaccact gccctgaggg
     7561 cgccatcatc caggacgaca tctactcggc cccgcgcatc gacgaggaca agtgcacgga
     7621 gtgcggcgtc tgcaccagcg tctgccgcgt gttctccatg gtccgggacg aggtcacggt
     7681 cggctgatgg ctcacttcga cctcatctgc aaggagtgcg gccatcgctt cgaactggtc
     7741 accggcggcg cgatccgcga gaagcagaag cgttgcaagg agtgcggctc ggagaacatc
     7801 cgccagtcgt tcctgagcta cctgcagaac ggcccgctct ccagccccac gtgcggagag
     7861 cagcggccga gcagcggcta cggctgaggc tgctgaccgg cggcgcgtcg cgccgtcatc
     7921 gcactacctg actcgaacgg gatacctgcc gcatccacag gtcgtggggt aagctactcg
     7981 gcagtcatgg acgttccgcc ccgcgatctc agcctggatg agttcatcgt cgagatgagc
     8041 ggccagccgg tcaaggctct cactcacgag cggctcatgc acctggccgg caggttgcga
     8101 ctgtcccacg ccctcgtcgg cgccagcgtg gccttctcgc gggacggcta cgcgcgcaac
     8161 ctcgtgtgcc ggacgtcgtc cttcgagctc ctggtgctct gctggcgacc gggccaggaa
     8221 tcgaccatcc atgatcacgc gggggcgctg aacgcgatcc gcgtctactc cggcgaactg
     8281 acctcgcgca ccttcacccg gaccagcggc gcggccgcag gggacggccc ggtggcgcag
     8341 gacggcgaag aacgcgtgcc tgcggacggc cacatggtcg gcctcgacag gaacggcatc
     8401 caccagctgg ccaacacctc ggggcaggac ctcgtcaccg tccacgtcta cgcgcccgcg
     8461 ctgatggagc tcaacgtcta ctccaccacg tcggcggagg tggagcggcg gccgttgcgc
     8521 tacaccctgg ccgacgacct cgcctgagac tcccggcacg gcccgcgccg ccgagccggt
     8581 cacgggcccg gttcggtcag tccatcgtct cggccagcag cccctgtctg gagagcgtgc
     8641 cgagaagcac ggaccggagg atgtcgagcg cgtcgatgac gcgctcgtcg gccagtgagt
     8701 agaggacgct cggcccctcc cgcctggcct ccacgaggtg gcgctcgcgc aggaccttca
     8761 ggtgccgcga cacggtgggc tgcgggacgt gcagcgcctc cgccagctcg tggacgtgca
     8821 atggctgatc ccgaagggcg tacagcatga ggatgcgttt cgggtcggag agtccctgac
     8881 agacgttggc gtgcaggagg atgacctcgc gttccagaga ctcgtccatc tgcggcgcac
     8941 cttcctccca cgaatgacca cccgccggga tgatatctcc gggcggcttc ggcgtgccaa
     9001 tgagcgtctg cacgctcgct ggctcgcacc aacggccggg ggccgcgacg gggttgcccg
     9061 tcgcggcccc cgccgcatct ctcggacttg caggctcttc cctacctgac ggtgagcctg
     9121 gccgagccga ccttggactg ggcgttgccc gaggcgtcgg tcgcgtacac gtaatacctg
     9181 tacgtccccc tggccagccc gcagcggaag ttcttggcga gcggcacgtt gacggtcacg
     9241 gtgccggcgc tcatggtctt gacgaccttg ccggcgctgt tcttgacctt gatcatcacg
     9301 gtcgcggtgc ccctggtggg ctcagggtcg gtgactctgt acttcaatgt cgccacgcgg
     9361 cccttgagca ccacggcgaa ccgggccgcc gtcgtcggct tcacggtgtc cttgacgatc
     9421 tcgaaagtga ccgtctgctg ggcctcaacg ttgccgttga tgtcctgggc ccagaccttc
     9481 accttgtgcg cgccggccgc caggggcatg tccttgtcgg tcgggatgct gatcgcagcg
     9541 ctgaggggct tgtcggcgat cgtggcaagc cgcaccaagc cgttgtcgaa ctcgtggtag
     9601 atgtaggcga taccctcgtc atcggtcgcc gtggccacga tcgtcgcggc gtcccagtac
     9661 tgagcgccgg cggcgggcac gtcggtcgtc gtgaccggcg ggttgtcgtc atccgccagg
     9721 cccgcgtagg cgccgacggc gccgaccgca agaacggcga cggccaggac gatcgccagc
     9781 accatcagat gtgtcttcct cactgtgcat acctccatca gcggagcgga gcagtgtgag
     9841 ctccgttccg gatcattgat ccaggcggat cgctcagttc atgtggccgt cgtcagtggt
     9901 gttgtcgtgg caggatccgc aggccatctt cgcgccggtg tacgtcttgt ggcacgcgcc
     9961 gcatgcagcg acgctgggat ccagacccac gggcggcgct tcagccagct cgaagtactt
    10021 gttgagcacc tcgatggtct tgcgggcgac gtcgaagcag gccttgccgc agcggtcgcg
    10081 ctgcagcggg ccgccgtcgg acgcgcccgt ggtcgtcgtc cactggacga gcgaagagtg
    10141 gcacagcggg gagttcgcgg tcgaggtggg tgcgttctcg atcatcgtgt agccgtccac
    10201 cgccggaggc gtccaggcgg gaaggccgag cgcgccttgt gacgacttga aggtgttgtt
    10261 cgtcggcaac ggggtctccg cgtagtactg gaagatgccg ttgatgaggt tgttgcggtg
    10321 cgtccatccc ggcttaccgt cgacgacgtt cgtgcctacg gccatgctcg tgaacgctgc
    10381 tgcgccattc agcgtgccgc agatggtccc ccagccggcg atgccgccgc cgccgaagcg
    10441 gaacatgttc atcggcaacg acgcccaatc ggcctggttc acaccgggaa tggcggcgag
    10501 ggcctcgacg caggagtacc agacggc
//
LOCUS       DASUMX010000081         5101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_741795, whole genome shotgun sequence.
ACCESSION   DASUMX010000081 DASUMX010000000
VERSION     DASUMX010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 5101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5101
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_741795"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            62..751
                     /locus_tag="VFZ86_03895"
     CDS             62..751
                     /locus_tag="VFZ86_03895"
                     /inference="COORDINATES: protein motif:HMM:NF044913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641466.1"
                     /translation="MTDPSQDKDAEDEAAATGEVDAPASAAPEGGAAPPQWQERDAWT
                     ALDDLDGSPTLVPASAWPAGAAGVDRPGLFAFWVDEAGAADLSRGLDLPLAAGRIYVG
                     QAGATKWPSGRANEDTLGDRVGRTHLAGKVRGSAVRLALAAVLLDRLGLVVQAAVLLQ
                     ASSEEALSDWMRTHLSVAVHVHDDRDTLGGLEQAVVERLDPPLNLESHLPATSVRQRV
                     AVLRKRISREG"
     gene            complement(735..1916)
                     /locus_tag="VFZ86_03900"
     CDS             complement(735..1916)
                     /locus_tag="VFZ86_03900"
                     /inference="COORDINATES: protein motif:HMM:NF024925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX5641467.1"
                     /translation="MFILEEPYVSDLLAATVAELGLPVLRTPIAERRLSGAHAALLRD
                     DAAFSAAACTPGARLYSNSENAIGWIAAHLSGSDLPRQIALFKDKVAFRDLVADLYPD
                     YRYAGVPADELRTFDPARLRAPFIVKPAVGFFSLGVHVVESAAAWPAVIEEIESEVKA
                     LGHLYPDQVLDLDHFVVEEVIEGEEFAVDAYYDADGAPVLVNAYAHLFSSADDVSDRV
                     YLTSAETVARLGPPAVEFLTEVGRRARLADFPVHTELRIDGDGRVAPIEVNPMRFGGW
                     CATDLAHFAYGVNPYRCYLRDEHPDWARIAEETAGRTTALIVSDLPASVDLAAIASVD
                     YEGFAARFSTVLELRPTDFNRYPVFAFTFVEVPSGDLSELHAVLGADLREHLRMVSPP
                     G"
     gene            2058..2765
                     /locus_tag="VFZ86_03905"
     CDS             2058..2765
                     /locus_tag="VFZ86_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641468.1"
                     /translation="MDNVGIDLKAEQLATLARPRYAYSSITRALVRGMDVFAGSGTTV
                     LKAKIVETLAPVPYRAWENREYGRLSRHPGDDTLVREAEAVLRWGREAQDKEYQHLRV
                     IDARLREDGTQVPLRLTRPMPDLMAGSWALASRTLSRTSLRRAFLLNAEAEDHAEHYY
                     AELVQLHPEWEEQPVTSAAALEYGAFASWADVFRRIGLDERDHMNASFAFAGKPELIV
                     SYEGMPPTPGPEPRAAA"
     gene            2851..3870
                     /locus_tag="VFZ86_03910"
     CDS             2851..3870
                     /locus_tag="VFZ86_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641469.1"
                     /translation="MSAASYSAFRRRRAAARVALRATSSAALLAAACLAAFVVLAAGA
                     AAPASAGPYVNPSPAEIRAKLQAAAAERLIPPKILYGIAFQESTWRQFDAKGDPLIGL
                     DGKGIGIMQVTTIPLGVDVERLKTDIDYNIAVGADILVTKWGYAPSVFPVIGGGDPRC
                     YENWFFAVWAYNGWIRGNPYPYKVWGHIADGRGLWTGLAVTPMPAAWLVGGFPVPPVA
                     TPQPEHWWSPTPLPKPVLSVPRVQKRVGVGDRFSVSGSLSPRHPAGANSVELRLSRWN
                     GSRWVLRRTVLTTNRDGAAADVTRWAATFSLAKTGRWKIVAYAPADADHAATTSKPGF
                     VTVVR"
     gene            4027..4311
                     /locus_tag="VFZ86_03915"
     CDS             4027..4311
                     /locus_tag="VFZ86_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015258670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HEX5641470.1"
                     /translation="MRWNAEKNELLRIERGVSFEAMVVAMGAGGLLDVLPHPNQARYP
                     GQRILVVAADRYVYLIPSVEEEQYIFLKTIIPSRKATGAYLHGGGRRAGS"
     gene            4298..4564
                     /locus_tag="VFZ86_03920"
     CDS             4298..4564
                     /locus_tag="VFZ86_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017319149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641471.1"
                     /translation="MPDLDAYEAEILEAYEKGELGSVATRDELARIREAARATAAKDR
                     RVNIRLTSGDLADIQAKALEEGIPYQTLIASVLHKYVSGRLVER"
     gene            complement(4573..>5101)
                     /locus_tag="VFZ86_03925"
     CDS             complement(4573..>5101)
                     /locus_tag="VFZ86_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641472.1"
                     /translation="ATWAARIRTAVATAVVFAPWDASAEDIAGGRAPSLLLTTDARRL
                     GPGVLAWWDWDEVTRREAAPFLEKTGAAELQTSAVYWRGFPVLQLATTEPPETDPAAP
                     LQLLGTLYTPEQTFSSLLVVPRGEEDPWIERMQEVMDGFFLLPIEREGRSRTGHQHVR
                     SLRLSASDLEDLAAR"
BASE COUNT          748 a         1791 c         1876 g          686 t
ORIGIN      
        1 tctcttgacg ggaacgcgct cgaggtgtca ggtggggagg ccctacagtc aggagaccgc
       61 cgtgaccgac ccatcccagg acaaagacgc cgaggacgaa gccgccgcga ccggcgaggt
      121 cgatgctccc gcaagcgccg cgccggaggg cggcgcggct cccccgcagt ggcaggagcg
      181 cgacgcctgg acggcgctcg acgacctcga cggctcgccg accctcgtgc cggcgagcgc
      241 gtggccggcc ggcgccgccg gcgtggaccg gccggggctc ttcgccttct gggtcgacga
      301 ggcgggcgcc gccgacctct cgcgcggcct cgacctgccg ctcgcggccg ggcgcatcta
      361 cgtggggcag gccggagcca cgaagtggcc ctccggccgg gccaatgagg acacgctcgg
      421 cgaccgcgtc ggccgcacgc acctcgccgg caaggtccgt ggctcggcgg tgcgcctggc
      481 gctcgcggcc gtccttctgg accggcttgg actggtggtg caggcggcgg tgctgctcca
      541 ggcgtcgtcg gaggaggcgc tcagcgactg gatgcgcacg cacctctccg tggccgtgca
      601 cgtgcacgac gaccgcgaca ccctcggcgg tctggagcag gcggtcgtcg agcgcctcga
      661 cccgccgctg aacctcgaga gccacctgcc ggcgacctcg gtgcgccagc gcgtcgccgt
      721 cctgcgcaag cggatcagcc gggagggctg accatcctca ggtgctcgcg caggtcagcg
      781 cccagcaccg cgtgcagctc cgagaggtcg ccggacggca cctcgacgaa cgtgaacgcg
      841 aacaccgggt agcggttgaa gtcggtcggg cgcagctcga gcaccgtgct gaagcgggcg
      901 gcgaagccct cgtagtcgac ggacgcgatc gccgcgaggt ccaccgaggc cggcaggtcg
      961 gagacgatga gcgccgtggt gcggcccgcg gtctcttccg cgatgcgggc ccagtcgggg
     1021 tgctcgtcgc gcaggtaaca gcggtagggg ttgacgccgt aggcgaagtg ggcgaggtcc
     1081 gttgcgcacc agccaccgaa gcgcatcggg ttgacctcga tcggcgcgac ccggccgtcg
     1141 ccgtcgatgc gcagctcggt gtggacgggg aagtcggcga ggcgggcgcg ccggccgacc
     1201 tcggtgagga actccacggc cggcgggccg aggcgcgcga cggtctccgc gctggtcagg
     1261 tagacgcggt cgctcacgtc gtcggcggag gagaacaggt gcgcgtacgc gttcacgagg
     1321 accggcgcgc cgtcggcgtc gtagtaggcg tcgacggcga actcctcgcc ctcgatgacc
     1381 tcctcgacca cgaagtggtc caggtccagc acctggtccg ggtacaggtg gcccagcgcc
     1441 ttgacctcac tctcgatctc ctcgatcacc gccggccagg ccgcggcgga ctcgaccacg
     1501 tgcacgccga ggctgaagaa cccgaccgcc ggcttgacga tgaacggcgc gcgcagccgc
     1561 gccgggtcga acgtgcgcag ctcgtccgcc ggcaccccgg cgtagcggta gtccgggtac
     1621 aggtcggcga cgaggtctcg gaaggcgacc ttgtccttga acagggctat ctgccgcggc
     1681 aggtcggagc ccgagaggtg ggcggcgatc cagccgatcg cgttctcgga gttggagtag
     1741 agccgtgcgc cgggcgtgca ggccgcggcg gagaaggccg cgtcgtcgcg gaggagcgcg
     1801 gcgtgcgcgc cggacagccg gcgctcggcg atcggcgtgc gcagcacggg cagcccgagc
     1861 tcggcgacgg tcgcggcgag caggtcggag acgtacggct cttcgaggat gaacacggtg
     1921 ggcaggctcc tggctcggtt cggcgggctg gcgacaggat agccgactcg gggactgggt
     1981 gcgggcgcaa acgggttagc cttggctgcg ttgggcactc ctcgtctagc aaacgtcaga
     2041 cgacggaaag gggacccgtg gacaacgtcg gcatcgacct caaggcggag cagctcgcga
     2101 ccctggcacg gccgcggtat gcgtactcat ccatcaccag ggcgctcgtc cgcggcatgg
     2161 acgtgttcgc cggctccggg accacggtgc tcaaggccaa gatcgtcgag acgctggcgc
     2221 cggtcccgta cagggcctgg gagaaccgcg agtacgggcg tctctcacgg cacccgggag
     2281 acgacacgct ggtgcgcgag gccgaggcgg tcctgcgctg gggccgcgag gcgcaggaca
     2341 aggagtacca gcacctgcgc gtgatcgacg ccagactccg cgaggatggc acgcaggtgc
     2401 cgctgcgcct cacgcggccg atgcccgatc tgatggccgg ctcgtgggcg ctggcgtcgc
     2461 gcactctgtc gcggacgtcg ctgcgccgcg cgttcctcct caacgccgag gccgaggacc
     2521 acgccgagca ctactacgcc gagctggtcc agctgcatcc ggagtgggag gagcagccgg
     2581 tgacgagcgc ggcggcgctg gagtacggcg cgttcgcctc ctgggcggac gtcttccgcc
     2641 gcatcggtct cgacgagcgc gaccacatga acgccagctt cgcgttcgcc ggcaagcccg
     2701 agctgatcgt gagctacgag ggcatgccgc cgacgccggg gccggagccg cgcgcggctg
     2761 cgtagacgcc gggcgcggcg cggcgcgctg gcgagtacac ttggcgctcg aagtagcacg
     2821 tcgcgtgccc acgaaagggg acccgtcacg ttgagcgccg cctcctacag tgccttccga
     2881 cggcgccggg ccgcggcccg ggtcgcgctg cgcgcgacct cctccgcggc cctcctcgcg
     2941 gccgcctgcc tcgcggcgtt cgtcgtgctc gccgccggcg ccgcggcgcc ggcgtcggcc
     3001 ggcccctacg tgaacccctc gccggccgag atacgcgcca agctccaggc cgcggcggcc
     3061 gagcgcctca tcccgcccaa gatcctctac ggcatcgcct tccaggagag cacctggcgc
     3121 cagttcgacg ccaaaggcga cccgctcatc ggcctcgacg gcaagggcat cggcatcatg
     3181 caggtcacga ccatcccgct gggtgtagac gtcgagcgcc tcaagaccga catcgactac
     3241 aacatcgccg tcggggcgga catcctggtc accaagtggg ggtacgcccc ctcggtcttc
     3301 ccggtgatcg gcggcggcga cccgcgctgc tacgagaact ggttcttcgc ggtctgggcg
     3361 tacaacggct ggatccgcgg caacccgtat ccgtacaagg tctgggggca catcgccgac
     3421 ggccgcggcc tgtggaccgg cctcgcggtc accccgatgc cggccgcctg gctggtcggt
     3481 gggttcccgg tgccgcctgt cgcgacgccg cagcccgagc actggtggag cccgacgccg
     3541 ctgcccaagc cggtgctgag cgtgccgcgc gtgcagaagc gggtcggggt gggcgaccgg
     3601 ttcagcgtct ccggctcgct gtcgccccgg cacccggccg gggcgaactc ggttgaactg
     3661 cgcctctccc gctggaacgg ctcgcgctgg gtcctgcgcc gcaccgtgct cacgacgaac
     3721 cgcgacggcg ccgccgccga cgtcacgcgt tgggcggcca ccttctcgct cgccaagacc
     3781 ggccgctgga agatcgtcgc ctacgccccg gcagacgccg accacgccgc cacgacctcc
     3841 aagccggggt tcgtcacggt cgtgcgctga cgggcgaccc cgcgggtcgc gcgcagcgcg
     3901 aagcgtcacc tcgccccacc cttcgtcgcc cttgtccggc gtggtcctgg gactcacgct
     3961 caggggactg tggacgacgg tgatgacaaa aggcacacag cgcggtatgg tcttctgcag
     4021 aagaccatgc gctggaacgc agagaagaac gagctcctga ggatcgaacg tggcgtttcg
     4081 ttcgaggcga tggtcgtcgc catgggcgcg ggtggactgc tcgacgtgct gccgcatccg
     4141 aatcaggcca ggtatccggg ccagcggatc ctcgtcgtgg cggccgaccg ctacgtgtac
     4201 ctgatcccgt ccgtggaaga agagcagtac atcttcctca agacgatcat cccgagccgc
     4261 aaggcgacgg gagcgtacct gcatgggggt ggacgccgtg ccggatcttg atgcctacga
     4321 agcggagatc ctcgaggcct acgagaaggg cgagctcggg tcggtcgcga ccagggacga
     4381 gctcgcacgg atcagggagg cggctcgcgc cacggcggcc aaggaccgcc gcgtcaacat
     4441 caggctgacc tcgggcgacc tcgcggacat ccaggcaaag gccctggagg agggcatccc
     4501 gtaccagacg ctcatcgcga gcgtgctgca caagtacgtc agcggccggc tggtcgaacg
     4561 ctgacggcag gttcagcgtg cggcgagatc ctcgaggtcg ctcgccgaga ggcgcagcga
     4621 ccgcacgtgc tggtggccgg tgcggctgcg cccctcgcgc tcgatgggca ggaggaagaa
     4681 gccgtccatg acctcctgca tgcgctcgat ccaggggtcc tcctcgccgc gcgggaccac
     4741 gagcagcgac gagaaggtct gctccggcgt gtagagcgtg ccgagcaact gcaggggcgc
     4801 cgccgggtcg gtctcgggcg gctccgtcgt cgccagttgc agcacgggaa agccccgcca
     4861 gtagacggcg ctggtctgca gctcggccgc gccggtcttc tccaggaacg gcgcggcctc
     4921 gcggcgcgtg acctcgtccc agtcccacca ggccagtacg cccggtccca gccgcctggc
     4981 gtcggtcgtc agcagcaggc tcggcgcgcg gccgccggcg atgtcctcgg cgctcgcgtc
     5041 ccagggtgcg aagacgacgg ccgtggcgac ggccgtccgg atgcgcgctg cccaggtggc
     5101 g
//
LOCUS       DASUMX010000082         2583 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1028870, whole genome shotgun sequence.
ACCESSION   DASUMX010000082 DASUMX010000000
VERSION     DASUMX010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1028870"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="VFZ86_03930"
     CDS             <1..245
                     /locus_tag="VFZ86_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641473.1"
                     /translation="ADLGEKAKPTTDKAKSEAAEIIDKAKKEALDLIERAKKEGPELL
                     QKAMKEGAELLEKAQKEANELLEKAKGKLGKQDDTQ"
     gene            232..861
                     /locus_tag="VFZ86_03935"
     CDS             232..861
                     /locus_tag="VFZ86_03935"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012851545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEX5641474.1"
                     /translation="MTRSDALAAGPDGRPRCFWAVGGAPEYVAYHDEEWGRPVLTDDG
                     LFERLTLEAFQSGLSWLTILRKRPAFRQAFAGFDVAAVAAFGDADRERLMADAGIVRN
                     RLKVDAALANARAALPVIEEHGSLAALLWRFRPQDHAAPRTRADWASTSPESMAMAKE
                     LKRRGFRFVGPTTAYSLMQAAGLVNDHAAGCCVHGAVEAEQLEAAARLA"
     gene            975..2513
                     /gene="purH"
                     /locus_tag="VFZ86_03940"
     CDS             975..2513
                     /gene="purH"
                     /locus_tag="VFZ86_03940"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEX5641475.1"
                     /translation="MKVKRALISVFNKSGVESFARALTDMGVTVISTGGTAKLLKDSG
                     IPVTLVEEVTGFPEMLDGRVKTMHPRLMAGVLARRDVPEHLQTIAEHDIEPIDMVVCS
                     LYPFEEVAGRRGVEDEVVIENIDIGGPSMIRAAAKNHAGVAVVTSHEDYEAILAELKA
                     SGELSYETLRELATKAFHRTAHYDFVIANWFSEAEDDFPHYVMRDYEKVLDLKYGENP
                     HQRAAYYSEVGLRQHLLSQVTQLHGKQLGFNNLYDLNAARSICDEFTLPCVTLIKHNN
                     PCGCALAENLAAAYDKAYACDPVSAFGSVIAVNRTVDVALAERLAELFVEVLFAPGYD
                     EAALEILQRKADIRILRNEERRQMNPGEFDYKRVTGGILVQDKDGDIEERDEMTVATR
                     RHPSEQEWGDLIFAFRVAKHVKSNAIVIARNLATLGVGAGQMSRVDSTRIAIDKARTG
                     LEGAVVGSDAFFPFPDAVDLAIDNGVTAFMQPGGSKGDETAVAACDARDAAMVFTGRR
                     HFLH"
BASE COUNT          451 a          919 c          876 g          337 t
ORIGIN      
        1 ccgcggatct cggcgagaag gccaagccca cgaccgacaa ggccaagtcc gaggcggcgg
       61 agatcatcga caaggccaag aaggaggccc tggacctgat cgagcgggcc aagaaggagg
      121 ggccggagct gctccagaag gccatgaagg aaggcgccga gctgctcgag aaagcgcaga
      181 aggaagccaa cgagttgctc gagaaggcca agggaaagct cggcaagcag gatgacacgc
      241 agtgatgccc tcgcggccgg ccccgacggc cggccgcgct gcttctgggc cgtcggcggc
      301 gcccccgagt acgtcgccta ccacgacgag gagtggggcc ggccggtcct gacggacgac
      361 ggcctgttcg agcggctcac gctcgaggcc ttccagtccg gactctcctg gctgacgatc
      421 ctgcgcaagc ggccggcgtt ccgccaggcg ttcgcgggct tcgacgtcgc cgcggtcgcc
      481 gcgttcggcg acgccgaccg cgagcgcctc atggccgacg ccggcatcgt gcgcaaccgg
      541 ctcaaggtgg acgcggcgct ggccaacgcg cgcgccgccc tgcccgtgat cgaggagcac
      601 ggatcgctgg cggcgcttct ctggcgcttc cggccccagg accacgcggc gccgcggacc
      661 cgggcggact gggcctccac gagccccgag tccatggcca tggcgaagga gctcaagcga
      721 cgcggcttcc gcttcgtcgg cccgaccacc gcctactccc tcatgcaggc ggccggcctg
      781 gtcaacgacc acgccgcagg ctgctgcgtc cacggcgccg tcgaggccga gcagctggag
      841 gcggcggcgc gcctcgcctg aggccgcgcg ggcattcctc gccgccgggc tttcttgtag
      901 gcttgcgcac aaggaacggg gcgccgtgaa acgcgcctcg ctcggggcag gcccacgtaa
      961 ggagagcagg cacagtgaag gtcaagcgag cactcatctc ggtcttcaac aagagcggcg
     1021 tcgagtcatt cgccagggcc ctcaccgaca tgggcgtcac cgtcatctcc acgggcggca
     1081 cggcgaagct gctcaaggat tccggcatcc cggtcacgct ggtggaagag gtcaccggct
     1141 tcccggagat gctcgacggc cgcgtcaaga cgatgcaccc gcggctcatg gccggtgtcc
     1201 tcgcgcgccg cgacgtaccc gagcacctgc agaccatcgc cgagcacgac atcgagccca
     1261 tcgacatggt cgtctgcagc ctctacccgt tcgaggaggt cgcggggcgg cgcggggtcg
     1321 aggacgaggt cgtcatcgag aacatcgaca tcggcggtcc gagcatgatc cgcgccgccg
     1381 ccaagaacca cgccggcgtc gccgtggtca ccagtcacga ggactacgag gccatcctgg
     1441 ccgagctcaa ggcgtcgggc gagctcagct acgagacgct gcgtgagctc gccaccaagg
     1501 ccttccaccg cacggcgcac tacgacttcg tcatcgccaa ctggttcagc gaggccgagg
     1561 acgacttccc gcactacgtg atgcgggact acgagaaggt gctcgacctc aagtacggcg
     1621 agaaccccca ccagcgcgcc gcctactact ccgaggtcgg tctgcgccag cacctgctct
     1681 cgcaggtgac gcagctgcac ggcaagcagc tcggcttcaa caacctctac gacctcaacg
     1741 ccgcgcgcag catctgcgac gagttcacgc tgccctgcgt gacgctcatc aagcacaaca
     1801 acccgtgcgg gtgcgcgctc gccgagaacc tcgcggccgc ctacgacaag gcctacgcct
     1861 gcgatcccgt cagcgccttc ggcagcgtca tcgccgtcaa ccgcaccgtg gacgtggcgc
     1921 tcgccgagcg cctggccgag ctgttcgtcg aggtgctctt cgcgcccggc tacgacgaag
     1981 cggccctgga gatcctccag cgcaaggccg acatccgcat cctgcgcaac gaggagcgcc
     2041 ggcagatgaa ccccggcgag ttcgactaca agcgcgtcac gggcgggatc ctcgtccagg
     2101 acaaggatgg cgacatcgag gagcgcgacg agatgacggt ggcgaccaga cgccacccga
     2161 gcgagcagga gtggggcgac ctcatcttcg ccttccgggt cgccaagcac gtaaagagca
     2221 acgccatcgt catcgccagg aacctggcga cgctgggcgt gggcgccggc cagatgagcc
     2281 gcgtggacag cacgcgcatc gccatcgaca aggcgcgcac cgggctcgag ggcgcggtcg
     2341 tgggctcgga tgcgttcttc ccgttcccgg acgccgtcga cctggccatc gacaacggcg
     2401 tcaccgcgtt catgcagccg ggcggctcca agggcgacga gaccgcggtc gcggcctgcg
     2461 acgcgcgcga cgccgccatg gtgttcacgg ggcggcggca cttcctccac tgaatcgaac
     2521 gagctgcggg gcggggaggc cggcagggcc gccccgcccc tcttcgtttg tgggcccact
     2581 ccg
//
LOCUS       DASUMX010000083         6339 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_166518, whole genome shotgun sequence.
ACCESSION   DASUMX010000083 DASUMX010000000
VERSION     DASUMX010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 6339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6339
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166518"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="VFZ86_03945"
     CDS             <1..456
                     /locus_tag="VFZ86_03945"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HEX5641476.1"
                     /translation="VREAARTLRDELEDRGLESYLRTSGGKGLHVVVPFTPRRHWPFV
                     GAFTREVVEALVAREPGRYTATMSKAKRGGKVFVDHFRNGRGATSVTSYSLRARPGAP
                     VAVPISWDELGRTRGGGDWTAERVLRRLRGRDDPWADFPATGRRQKLEE"
     gene            462..1166
                     /locus_tag="VFZ86_03950"
     CDS             462..1166
                     /locus_tag="VFZ86_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006942987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkoF family
                     thiamine/hydroxymethylpyrimidine-binding protein"
                     /protein_id="HEX5641477.1"
                     /translation="MFTHSPAFAGFSVDDIASARTFYADTLGLEVSEANGMLTLHLAG
                     GGRVLVYPKEEHEPATFTVLNLPVDDIDGAVDALGARGVVFERYEGAPHDEKGILRPP
                     SPEYGPPIAWFKDPAGNILSVLQTAADEAAGDDAAGGGEVHLQLSVYPLRQPHLRPAI
                     EAALRAAAGEGVDVAVGRLSTLVRGDEPAVFAALRAAFRAAGSSGSTVMVATLAGGAP
                     SDETLAGIQAVVTGED"
     gene            1243..1893
                     /locus_tag="VFZ86_03955"
     CDS             1243..1893
                     /locus_tag="VFZ86_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5641478.1"
                     /translation="MSDSGTRAPIRVVVVDDHELVRGGLATILGLYDDIVLAGEADSG
                     AAAVRLCAEAQPDVVLMDLVLPGGMDGAEATEQILRTCPGAKILALTSFSDPDLIQRV
                     LRAGALGCLLKNVSGRELAEAIRRADAGTSTLAPEAADALVHAISSPVEVRAGLTRRE
                     RDVLKLMVEGLTNAEIAERLVVSLSTVKTHVSSIIAKLGASTRTEAATIAVREHLD"
     gene            2079..2498
                     /locus_tag="VFZ86_03960"
     CDS             2079..2498
                     /locus_tag="VFZ86_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641479.1"
                     /translation="MTTRTTTRITGTLNPWGPVSQTKRVRSYQEGRICAHAACGTILS
                     IYNPSKYCTVHAALGAGGRRRGLPQPIREVACEHCGTVFATGNQTRKYCSDRCRMAAF
                     ARRKRAALRAADSPAPVQALQTAAAPGELREPVGSAA"
     gene            complement(2528..6046)
                     /locus_tag="VFZ86_03965"
     CDS             complement(2528..6046)
                     /locus_tag="VFZ86_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641480.1"
                     /translation="MNRRIVRLAALTASLLLLALAAGVVAGAVTRSDVAYFDGFQDLT
                     GVDLSQSSGVQIDSLGGLRMATIGKATAATWTSAADFTTPAPPLGPVFGLSTLDAATV
                     SGTLRLPQTPLAFRRAAAEPVLEPVDALSVDGFSVGGMSVQRVSGDGDKYYMWYAGVP
                     ENEFAQRIYLATSTDGTTWTKEPEPVLGLGEPGAFDSRQLTKPSVIYDPLNAEAPFRM
                     WYAAEDETKGGVGYATSLDGRTWTKAGEVLPPGKPGMPDSHRVMQPCVLIDNGVYFMW
                     YTADDAENRRVAYATSADGLVWQRGGVVFDVGAGNYSEGAFAPAVVRTATGFHMLFTG
                     NKVVAGGDIQSKLINADSPDGLTWAAGNIAFSASGGDTAFDGYNVSQPTILSDPADAA
                     HPYKMWYVGNNPDANGNYHDRIGLAYQKSPGTVTQWVKAPGAAGDPYYESVLTLGTQG
                     SAFDTMKVADLRPVAKPASAGTGLYGFYTGTNAADFVSRIGVKESSDDGLTWTDAGTH
                     ATLIDKGAAGAFDEGGVACPAPVEDPAGGWWVYHTSLSLTGTSSIGLHTVPEDLSTPA
                     RNPSPVLASGAAYDAAGQADPCVVADGPALVLLYAGKDVSGTWSIGSATTTTAAPATF
                     SAAHQVLAPEPATYAYDAGGLRHPVAHKAPDGSWRLFYTAIGTDGVRRIAYATAPADL
                     SSWTRRGLVMDASPDAYDFTEAGVEPSAASALDPAGETLYFTGTDRFGWTRVGKATAA
                     GAGFVATGSATYELDGGSVRDWRRIAWAPAATPEGTTREVHVSYYPTVTGGWSDFYEV
                     AGDTDLPFQLTVQKMRWQVRMTSSDAAASPTLEQLTVNHAPVQFPAAATAATVPLGPP
                     DGLYLLTWGDLSVSCDAPAGSGLTVAVKDDAGATLVPAGQYLVGSGVTTIPLAGVAAP
                     GGRLVAVIGFTGDGATTAKVKNLTASYTTTATPSQMTLSAAKTLLTYGATTTLSGKLV
                     SDPTPLDATNGDAVAMAGQTVTISANVAGTTGYTNPVTVTTGADGSFVLPTPVKPAAT
                     TSYRAVWEGATVADVTYPPASASVRVQVKPKVTLALTRYNRRSGKYFLYKAGRTVYAK
                     GAMAPNHARLGDGTTAGKVTVTAYRYTSRKWVKVKASARALTTTSTYTWSWRPRYKGT
                     YRWATSFAGDVDHTAAVSPFRYVKIY"
     gene            complement(6151..>6339)
                     /locus_tag="VFZ86_03970"
     CDS             complement(6151..>6339)
                     /locus_tag="VFZ86_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641481.1"
                     /translation="VMRAGQAQVQAAPGAASGDGEERVGSLRLFRGCIAAIVLGVFAA
                     VILPLLVWAAAILFAGAR"
BASE COUNT          859 a         2246 c         2358 g          876 t
ORIGIN      
        1 gtgcgcgagg cggcgcgcac gctccgcgac gagctcgagg accgcgggct cgagagctat
       61 ctgcgcacct ccggcggcaa gggcctgcac gtggtcgtgc ccttcacgcc gcgccggcac
      121 tggccgttcg tgggtgcgtt cacgcgtgag gtggtcgagg cgctggtcgc gcgcgagccc
      181 ggcaggtaca cggcgacgat gtccaaggcc aagcgcggcg gcaaggtgtt cgtggaccac
      241 ttccgcaacg ggcgcggcgc gacctccgtg acctcgtact cgctgcgcgc gcggcccggc
      301 gccccggtgg ccgtgcccat cagctgggac gagctgggca ggacgcgcgg cggcggcgac
      361 tggacggccg agcgggtgct gcggcgcctg cgcggccgcg acgatccctg ggcggatttc
      421 ccggccaccg gccgcagaca gaagctggag gagtgatcac gatgttcacg cacagccccg
      481 cgttcgccgg attctcggtc gacgacatcg cgagcgcgcg cacgttctac gccgacaccc
      541 tcgggctcga ggtcagtgag gcgaacggga tgctgaccct gcacctcgcc ggcggcggca
      601 gggtcctggt ctaccccaag gaagagcacg agccggcgac gttcacggtg ctgaacctgc
      661 cggtggacga catcgacggc gccgtcgacg ctctgggcgc gcgcggcgtc gtcttcgagc
      721 gctacgaggg cgcgccgcac gacgagaagg gcatcctgcg gccgccgagc cccgagtacg
      781 ggccgccgat cgcgtggttc aaggatcccg ccggcaacat cctctcggtg ctgcagacgg
      841 cggcggacga ggcagccggg gacgacgcgg ccggcggcgg ggaggtgcac ctgcagctct
      901 ccgtgtaccc gctgcgccag ccgcacctga ggcccgccat cgaggcggcg ctgcgcgccg
      961 cggccggcga gggcgtggac gtcgccgtcg gccgcctcag caccctggtg cgcggagacg
     1021 agccggccgt gttcgccgcc ctgcgggcgg cgttccgcgc ggccggctcg tccggctcca
     1081 cggtgatggt cgcgacgctc gccggcggcg cgccgtcaga cgagacgctc gccggcatcc
     1141 aggccgtcgt caccggtgaa gactgacgtc cccccgaagc actgcggtca tggtacggtt
     1201 gacggcgtcg agggagcctc cgggggagga agcggggaca cgatgagcga cagcggcacg
     1261 cgagcaccca tcagagtggt cgtcgtcgac gatcacgagc tggtgcgcgg cgggctcgcg
     1321 acgatcctcg gcctctacga cgacatcgtc ctcgccggag aggccgactc gggggcggcg
     1381 gccgtccggc tctgcgcgga ggcgcagccc gacgtggtgc tcatggacct cgtcctgccg
     1441 ggaggcatgg acggggcgga ggcgacggag cagatcctcc gcacctgccc cggcgccaag
     1501 atcctggcgc tcacgagctt ctcggacccc gacctcatcc agcgcgtgct gcgcgccggg
     1561 gcgctcggct gcctgctcaa gaacgtgagc ggccgggagc tcgccgaggc gatccgccgg
     1621 gcggacgccg ggacgagcac cctggcgccg gaggcggcgg acgccctcgt ccacgccatc
     1681 tcctccccgg tcgaggtgcg cgccggcctc acgcggcgcg agcgcgacgt gctcaagctc
     1741 atggtcgagg ggctgaccaa cgccgagatc gccgagcgcc tggtcgtcag cctgtccacc
     1801 gtcaagacgc acgtgagcag catcatcgcc aagctgggcg cgtccacgcg caccgaggcg
     1861 gccaccatcg ccgtccgcga acacctcgac tgacgcttcg gcgccggtcg cgcatcggtc
     1921 acctgaccca gagaagaccc gtccaccggc ccccccaagt gtgtccgcct gcccgatgcg
     1981 ccgcggcgcg tcgctacgta cgctggcctg gcttccatca cctgcggggc atcactctgc
     2041 gatgccgccg cgaggatcgc cgccggaggg tgcgcctcat gaccacacgc acgacgacac
     2101 ggatcacagg gacgctcaac ccgtggggac ccgtctccca gacgaagcgg gtccgctcct
     2161 accaggaagg gcgcatctgc gcgcacgcgg cgtgcgggac gatcctctcg atctacaacc
     2221 cgtcgaagta ctgcaccgtg cacgccgcgc tcggggccgg cggccggcga cgggggctgc
     2281 cccagccgat ccgcgaggtg gcctgcgagc actgcggcac ggtgttcgcg accggcaacc
     2341 agacgcgcaa gtactgcagc gaccgctgcc ggatggcggc cttcgcccgg cgcaagcgcg
     2401 cggcgctgcg cgccgcggac tcgccggcgc cggtccaggc cctccagacg gccgcggctc
     2461 ccggcgagct tcgtgagccc gtcgggagcg ccgcctgaca gaggacgggc gcccggcgcc
     2521 ccgcccgtca gtagatcttc acgtagcgga aggggctgac ggccgcggtg tggtccacgt
     2581 cgccggcgaa gctcgtcgcc cagcgatacg tgcccttgta cctcggccgc caggaccacg
     2641 tgtaggtact ggtggtggtg agcgcgcgcg cggaagcctt caccttgacc cacttgcgcg
     2701 acgtgtaccg gtaggcggtg acggtgacct tccccgcggt cgtgccgtcg ccgagcctgg
     2761 cgtggttggg cgccatggcg cccttggcgt acacggtgcg ccccgccttg tagaggaagt
     2821 acttgccgct cctcctgttg tacctggtga gcgccagggt gaccttgggc ttcacctgga
     2881 cgcgcaccga ggcggaggcc ggcgggtagg tgacgtcggc gacggtggcg ccctcccaca
     2941 ccgcgcggta gctcgtggtc gccgccggct tcaccggcgt cggcaggacg aaggagccgt
     3001 cggcgccggt ggtgacggtc acggggttcg tgtagccggt cgtcccggcg acgttggcgc
     3061 tgatcgtcac cgtctggccg gccatcgcga ccgcgtcgcc gttggtcgcg tcgagcgggg
     3121 tcggatcgct gaccagcttg cccgagagtg tcgtcgtggc gccgtacgtg agcagcgtct
     3181 tcgccgccga cagcgtcatc tgcgacggcg tcgcggtcgt cgtgtacgac gccgtcaggt
     3241 tcttcacctt ggcggtcgtc gcgccgtcgc cggtgaaccc gatgacggcc acgagcctgc
     3301 cgcccggcgc cgcgacgccc gccagcggga tagtggtcac gccggacccg acgaggtact
     3361 gaccggcggg gaccagcgtg gcgccggcgt cgtccttgac cgccaccgtg aggccgctgc
     3421 cggccggtgc gtcgcacgag acggagagat cgccccaggt gaggaggtac agaccgtccg
     3481 gcggacccag cggcacggtc gcggccgtcg ccgcggccgg gaactgcacc ggtgcgtggt
     3541 tcaccgtcag ctgctcgagc gtcggcgatg ccgcggcgtc cgagctggtc atgcgcacct
     3601 gccagcgcat cttctgcacg gtgagctgga agggcagatc ggtgtcgccg gcgacctcgt
     3661 agaagtcgga ccagccgccg gtgaccgtcg ggtagtaact cacgtggacc tcacgcgtgg
     3721 tgccttccgg cgttgccgcc ggggcccacg cgatccgccg ccagtcgcgc acgctgccgc
     3781 cgtcgagctc gtaggtggcg gagccggtcg cgacgaagcc ggcgccggcg gccgtggcct
     3841 tgccgacgcg ggtccagccg aagcggtcgg tgccggtgaa gtagagcgtc tcgccggccg
     3901 ggtcgagcgc cgaagcggcg ctcggctcca cgccggcctc ggtgaagtcg taggcgtcgg
     3961 gagaggcgtc catgaccagg ccgcgccgcg tccaggacga gaggtctgcc ggcgcggtgg
     4021 cgtaggcgat gcgccgcacg ccgtcggtgc cgatggccgt atagaagagg cgccagctgc
     4081 cgtcgggcgc cttgtgcgcg accgggtgac gcaggccgcc ggcgtcgtag gcgtaggtcg
     4141 ccggctccgg cgcgaggacc tgatgcgccg cggagaacgt cgcgggcgcc gccgtggtgg
     4201 tggtcgccga cccgatcgac cacgtgcccg agacgtcctt gcccgcgtac aacagcacca
     4261 gcgccggccc gtcggcgacg acgcacgggt ccgcctgacc ggcggcgtcg taggcggcgc
     4321 cggaggcgag caccggcgac gggttccgtg ccggcgtcga gaggtcctcg gggacggtgt
     4381 ggagaccgat cgaggacgtt cccgtcaggc tcagcgaggt gtggtagacc caccagccgc
     4441 cggcagggtc ctcgaccggc gccgggcagg ccacgccgcc ctcgtcgaac gcgcccgccg
     4501 cgcccttgtc gatcagcgtc gcgtgcgtgc cggcgtcggt ccacgtcaga ccgtcgtcgg
     4561 acgactcctt gacgccgatg cgcgagacga agtcggcggc gttggtgccc gtgtagaagc
     4621 cgtagaggcc cgtcccggcc gaggccggct tggcgacggg ccgcaggtcc gcgaccttca
     4681 tcgtgtcgaa cgccgatcct tgcgtcccca gggtgagcac ggactcgtag taagggtcgc
     4741 ccgccgcacc cggcgccttg acccactggg tgacggtgcc ggggctcttc tggtaggcga
     4801 gaccgatgcg gtcgtggtag ttgccgttgg cgtcggggtt gttgcccacg taccacatct
     4861 tgtacgggtg agcagcgtcc gcggggtcgc tgaggatcgt cggctgcgag acgttgtagc
     4921 cgtcgaaagc cgtgtcgccg cccgaggcgc tgaaggcgat gttgcccgcc gcccaggtga
     4981 ggccgtccgg gctgtccgcg ttgatgagct tgctctggat gtcgccgccg gcgacgacct
     5041 tgttgccggt gaacagcatg tggaagccgg tcgcagtgcg cacgaccgcc ggcgcgaacg
     5101 cgccctcact gtagttgccg gcgcccacgt cgaacacgac gccgccgcgc tgccagacaa
     5161 ggccgtcggc ggacgtggcg taggcgacgc ggcggttctc cgcgtcgtcg gccgtgtacc
     5221 acatgaagta cacgccgttg tcgatgagca cgcacggctg catcacccgg tggctgtcgg
     5281 gcatcccggg cttgcccgga ggcagcactt cgccggcctt ggtccaggtg cggccgtcca
     5341 gggaggtcgc gtaccccacg ccgcccttgg tctcatcctc ggcggcgtac cacatgcgga
     5401 acggcgcttc ggcgttcagg ggatcgtaga tcacgctggg cttggtcagc tggcgcgagt
     5461 cgaacgcgcc gggttcaccg agcccgagca ccggctccgg ttccttggtc cacgtcgtgc
     5521 cgtcggtgga ggtggcgagg tagatgcgct gggcgaactc gttctccggg acgcccgcgt
     5581 accacatgta gtacttgtcg ccgtcgccgc tgacgcgctg gacgctcatg ccgccgacgc
     5641 tgaagccgtc cacgctgagg gcgtcgacgg gctcgagcac cggctccgcg gccgcgcgcc
     5701 ggaacgcgag cggagtctgg ggcaggcgca gggtcccgga gacggtcgcg gcgtcgagcg
     5761 tggacaggcc gaagacggga ccgagcggtg gcgcgggcgt cgtgaagtcg gccgcgctcg
     5821 tccaggtggc ggccgtggcc ttgccgatgg tcgccatgcg caggccgccg agagagtcga
     5881 tctgcacgcc gctgctctgc gagaggtcga cgccggtcag atcctggaac ccgtcgaagt
     5941 acgcgacgtc tgacctggtg accgcgccgg cgacgacgcc ggcggccagc gccaagagca
     6001 gcagactcgc ggtcagggcc gcgagccgca cgatacgcct gttcatgcca ggacctcccc
     6061 cggttggacg ttcaacgcgc ggcgccccct ccggcgccgc ccgcctccct gttatcggcc
     6121 gggggccgag ttcctgtacg ggcgagcccc ctaccgcgcg ccggcgaaga ggatggcggc
     6181 cgcccacacc agcagcggaa gaatgacggc ggcgaagaca ccgaggacga tcgcggcgat
     6241 gcagccgcgg aagaggcgca gggagccgac gcgctcctcc ccgtcgccgg aggcggctcc
     6301 cggcgccgcc tgcacctgcg cctgccccgc ccgcatcac
//
LOCUS       DASUMX010000084         9891 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_454666, whole genome shotgun sequence.
ACCESSION   DASUMX010000084 DASUMX010000000
VERSION     DASUMX010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 9891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9891
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454666"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..606)
                     /locus_tag="VFZ86_03975"
     CDS             complement(88..606)
                     /locus_tag="VFZ86_03975"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX5641482.1"
                     /translation="MARARGTDLVIVVVALAVAALATVGVFVLGSQGAADRSPVSRGK
                     AIFQSGVDADGDLIPRSSSGGGMMSEGGMMGGGGMMGGGCATCHGTDGRGRTSAAFTA
                     PNITYDNLTDPRGMLEPDGGRGPTFSDATLRTAVIEGLDPEGSRLEAPMPQWQLTGSE
                     WSDLLAYLKTLR"
     gene            complement(608..871)
                     /locus_tag="VFZ86_03980"
     CDS             complement(608..871)
                     /locus_tag="VFZ86_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEX5641483.1"
                     /translation="MWNNNGHWGSGDWATLALIVIAVIAVIVAIVFLVRYLSQASGGT
                     SSTAAAPPSAPASGPESPKDILKRRYAAGEIDRDEYLQRLGDL"
     gene            complement(1032..1820)
                     /locus_tag="VFZ86_03985"
     CDS             complement(1032..1820)
                     /locus_tag="VFZ86_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641484.1"
                     /translation="MKLSFKALLTALAVVALAALLLVPIAGASPSDMMGDGDHDGDHS
                     GDWCGGGVWNGDGHWGGTGMWGTASGAAWLTSHPDALAAWTQLRVDHQQAMQTWHETY
                     KADLKSSAAQQALHDLWTAHWNAMKAFYEQYGGGAAWTCPSDGMWGGWDNGGMMGHEW
                     DASHMWGAGHGAAWMANHPRAFGRWLDMRAEQTAHVDRWAERYHKRLKSDAAQTALKT
                     MRAHHRAQVKTFYAKHHLSVTTARMRDGAGGWMGLGGMWGGFGW"
     gene            complement(1921..3129)
                     /locus_tag="VFZ86_03990"
     CDS             complement(1921..3129)
                     /locus_tag="VFZ86_03990"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5641485.1"
                     /translation="MSSPPATAPDLVRGLRAGRRDAFERLYGEYHAPIYNLCARVLGD
                     REEAKDVTQEVFIKAFDQLPQEDEESFRLRAWLYRVATNACFNHLRSRRKLDRGGDAG
                     LEQAASGVDEFERARTVALVEQSLGEMNERYRTALVLKDLQGLPPEEIAEVMEISRPN
                     ADVLVHRARASFKTVFAKLAGADAAAPAALGLVLMPLSVPAALLAMPPLPAGLAPVHA
                     LPSPDIASAAGPAGAGLLAKIGAAVTTKAAVTAAAAAVVIGGGAVAVKEGARSERVPA
                     AAATSAPVGAGSQTPSPYTAMHDRHRAGHGARWGEHGEAARHAGSAGHDSDGGHDAAT
                     HDSTATSTHDAGDAGGSTTTHDAAGDSTTTTHDGGTSTTDSTSTHSTSTTTTHDGGDT
                     SGSHDGGTGD"
     gene            complement(3240..3680)
                     /locus_tag="VFZ86_03995"
     CDS             complement(3240..3680)
                     /locus_tag="VFZ86_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641486.1"
                     /translation="MKVLKTIVATAVVVFAMTSVAMAGVQQLTKAQDDQAADKTRAQH
                     TVTLTDKQLARLIDHQGSHVREAQRTKTHARRVERKHAQAQQGAHEAEARHVERVRNS
                     VQSEQSDHSGSASPSGSHHYETSQQTHASSGGHDGGHGGGHGGD"
     gene            3679..4059
                     /locus_tag="VFZ86_04000"
     CDS             3679..4059
                     /locus_tag="VFZ86_04000"
                     /inference="COORDINATES: protein motif:HMM:NF015789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641487.1"
                     /translation="MVSPWVRCPFTCLNTGRRRFLHRLRRNIERLPGPAWSRLTLESD
                     DRVYTPSDLLPVFADTGVAAGLRSASPPLPADGLSVAEIDRCARKTWPSRRFGLEVEA
                     KAKELAVARLIADPDGEGARRESG"
     gene            complement(4067..4288)
                     /locus_tag="VFZ86_04005"
     CDS             complement(4067..4288)
                     /locus_tag="VFZ86_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641488.1"
                     /translation="MLDDATALDADDVANGPLRVSEQEAAETVWALTSPELHRLLVRL
                     RGWSQRRYRDWLAASLAELLLPGAGPSSS"
     gene            complement(4413..4757)
                     /locus_tag="VFZ86_04010"
     CDS             complement(4413..4757)
                     /locus_tag="VFZ86_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5641489.1"
                     /translation="MSDEEMAPETKGVTVESLAAVDLGPEIEGMAGRRLRMRMVTIQP
                     GGVFGPMHDHKGRPGTVYILQGTITDHRDGTATDYGPGVGWPEDRTTTHWLENRGTIP
                     AVEISVDIVSRE"
     gene            complement(4783..5166)
                     /locus_tag="VFZ86_04015"
     CDS             complement(4783..5166)
                     /locus_tag="VFZ86_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX5641490.1"
                     /translation="MADPGSVTVETLKMIADAFNRHDLDAIMEFFAEDCSMDLPRGPD
                     AWGQRFVGKVAVREGVASRFAGLPDVHYNDDRHWVSGDLGVSEWLLTGTRPDGTKVQV
                     RGCDHWEFRGGKVIRKDSYWKIVEK"
     gene            complement(5197..5490)
                     /locus_tag="VFZ86_04020"
     CDS             complement(5197..5490)
                     /locus_tag="VFZ86_04020"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEX5641491.1"
                     /translation="MAIRVIIEFHARPGRREELRSLLESVDAKSGPSAPGYLGSTRYA
                     VLEDPDALIEIADWETAEARTTAVQAVMASGVYGPLAELLAAPLKATVVRQLS"
     gene            complement(5534..5953)
                     /locus_tag="VFZ86_04025"
     CDS             complement(5534..5953)
                     /locus_tag="VFZ86_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008092072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX5641492.1"
                     /translation="MSREDDVRAISDRFYTGLSRMDVAALPEIWSHSDDVTTMHPMGG
                     EQVGWEEVRASFEQAAGPMTDSHVELVDQRVYAGTDLAFETGIERGSARLAGEPIEFE
                     HRVTNVYRLEDGQWKMVHHHTDTSPGLAEMLQRRQAA"
     gene            6424..6762
                     /locus_tag="VFZ86_04030"
     CDS             6424..6762
                     /locus_tag="VFZ86_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020116890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641493.1"
                     /translation="MVDLRDELDGYTVSFTSLLQDIDATPFMKGLPDDRCQCPHWGYV
                     FKGKMTARYADHDEVFEAGDAFYIPPGHIPVENEPGTEFLWFSPSEELRTAEAAMMKN
                     MQAMQDGSTS"
     gene            complement(6920..7174)
                     /locus_tag="VFZ86_04035"
     CDS             complement(6920..7174)
                     /locus_tag="VFZ86_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641494.1"
                     /translation="MLTRWYAPARPPATTRASYLVDTLALFLVPGFGEAISAYVVIPA
                     AIAEVWMVGYLLVIGVRITKPAVRPALECGAPGQPACALP"
     gene            7335..8057
                     /locus_tag="VFZ86_04040"
     CDS             7335..8057
                     /locus_tag="VFZ86_04040"
                     /inference="COORDINATES: protein motif:HMM:NF018798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HEX5641495.1"
                     /translation="MLHGTVASLLEGRRWLRLPVLRSAVSFIEMTALSLRLHRLNGGR
                     RGVRLIVCLVMCVLAGIALGSAVHALVGSQMLSGLVMQASGMLLALAVLQVGMGAEVW
                     RYHGAEHKVVNAYEARVDLRDVAAVKRFSRIHDRCGTNLVAIVFALALLCLPMGTSAL
                     AQALSLVTAVLVIAVALELFRLIERSPHARFSRVLLSGGRTLQRLLTTREPRAEHLHL
                     ASRALLEVIEAESAKSQAAPAS"
     gene            complement(8100..9230)
                     /locus_tag="VFZ86_04045"
     CDS             complement(8100..9230)
                     /locus_tag="VFZ86_04045"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HEX5641496.1"
                     /translation="MKRHLVATLLCTSLVLAFGLVATAAAGAAAGAAPYDLYFGDMHA
                     HTDYSDGTGTPWQAYTAAKAAGADFFALTDHTHYGYGTGWQTPSRYAETVALSEAFTD
                     ETFVAFAGYELWLPAVGEMNVFNTTEMFGQSGNPGDRGYNNGIRQSIRRVLPDLYAWL
                     GETGAVGQWNHPGLYGAQGGSSLVDFFHFDWWTPQRDEGVTMVEVWNEEIYEDEYFEA
                     LDAGWHVLPSANSDTHAADWITGCDVRTVLLSEDLTREGLLGALRASRGYATMDEDLE
                     VRYTLDGAVMGSDLAPKASYEAKIEIKDPDGAGDEVKLVEIVADGGEVVASKAFDEAE
                     AEWTTVVTSKEERYFLVRVTTASDFWGNEGVTAWTAPVWTGR"
     gene            9429..>9891
                     /locus_tag="VFZ86_04050"
     CDS             9429..>9891
                     /locus_tag="VFZ86_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641497.1"
                     /translation="MQRRALTLAAGHVRRSRLVGFVGAVCAVLLLTTPALAASSGQRL
                     WVRTYRPASGGATFSDVAAGARGAAYVAGSKRIDQSLALILLKYDGGGRRTWAHAFRG
                     DYRLTTGTAVGLDARGNVYVGGDVRWRNGESGILLVKYASDGTRRWSRTWNP"
BASE COUNT         1357 a         3443 c         3422 g         1669 t
ORIGIN      
        1 gagtgagcct gcgccgcgcc gccgccagtc tagcgtcccc gacccggaga cgcctgaggg
       61 cagggtggtc gactgggccg acccgcgcta tctcagcgtc ttcaggtagg cgagcaggtc
      121 gctccattcg gagccggtca gctgccactg cggcatcggc gcctccaggc ggctgccctc
      181 ggggtcgagg ccctcgatga cggccgtgcg cagggtggcg tccgagaacg tgggcccacg
      241 accgccgtcc ggctcgagca tgccccgtgg gtccgtcagg ttgtcgtagg tgatgttggg
      301 cgcggtgaac gccgcgctcg tccgtccgcg gccgtcggtg ccgtggcagg tcgcacagcc
      361 gccacccatc atgcccccgc cacccatcat gccgccttcg ctcatcatgc cgccgccgct
      421 cgagctgcgt gggatgaggt cgccgtcggc atcgaccccg gactggaaga tggccttgcc
      481 tctggatacc ggcgaccggt ccgccgcacc ttgggagccc agcacgaaga cgccgaccgt
      541 cgccagcgcg gcgacggcga gcgcgaccac gacgatcacg aggtcagtgc cgcgcgcacg
      601 agccatgcta gaggtcgccg agcctctgca ggtactcgtc gcggtcgatc tcgccggcgg
      661 cgtagcggcg cttgaggatg tccttgggcg actcaggccc gctcgctggt gccgacggcg
      721 gggccgctgc cgtggacgat gtgcctccag aagcctggct gagatagcgc acgaggaaga
      781 cgatcgccac gatgacggcg atcacggcga tgacgatgag ggcgagcgtc gcccagtcgc
      841 ccgaacccca gtgtccattg ttgttccaca tcatcgtcgg gaatcgcctg tctcggcctg
      901 cggaatcttc tacccttcac catgcccgag gactccggac accaagccgg tgcgaccccg
      961 gcgggaggat ggataccacc cggccggggt cgctggtgtt gcgtccgctc gcgcgggtca
     1021 gagcgcggcg tttaccagcc gaagccgccc cacatgccgc cgagtcccat ccagccgccg
     1081 gcgccgtcgc gcatgcgggc ggtggtcacc gacaggtggt gcttcgcgta gaaggtcttg
     1141 acctgggcgc ggtgatgcgc gcgcattgtc ttcagagccg tctgcgccgc gtcgctcttg
     1201 aggcgcttgt ggtagcgctc agcccagcgg tcgacgtgcg ccgtctgctc ggcgcgcatg
     1261 tcgagccagc ggccgaaggc gcgaggatgg ttcgccatcc aggcggcgcc gtgtccggcg
     1321 ccccacatgt gggaggcgtc ccactcgtgg cccatcatgc cgccgttgtc ccagccgccc
     1381 cacatgccgt cggaggggca ggtccaggcg gcgccgcctc catactgctc gtagaaggcc
     1441 ttcatggcgt tccagtgcgc tgtccacaga tcgtgcaggg cctgctgcgc cgcggagctc
     1501 ttcaggtcgg ccttgtaggt ctcatgccag gtctgcatcg cttgctggtg gtcgacgcgg
     1561 agctgcgtcc aggcggccag agcgtcgggg tgactcgtca gccaggcggc gcccgaggcg
     1621 gtgccccaca tgccggtgcc gccccagtgg ccgtcgccgt tccacacgcc gccgccgcac
     1681 cagtcgccgc tgtgatcgcc gtcgtggtcg ccgtcgccca tcatgtcgct cgggctggcg
     1741 ccggcgatcg gcacgaggag cagcgccgcg agcgcgacga ccgcgagggc ggtgagaagc
     1801 gccttgaagc tcagtttcat caggtgacca tcctttggct catcgagggt gtggtgcttc
     1861 cgctatctgg aacacgcgag gccggcgttt cttacagtca cgctccgcga aagcggcgaa
     1921 tcagtcgccc gtgccgccgt cgtggctgcc ggacgtgtcg ccaccgtcat gggtggtcgt
     1981 cgtgctggtc gaatgggtgc tggtggagtc cgtggtgctc gtgccgccgt cgtgcgtcgt
     2041 cgtcgtggag tcgccggcgg cgtcgtgcgt cgtggtcgag ccgccggcat cacccgcgtc
     2101 gtgggtggac gtcgctgtgg agtcatgcgt cgcggcgtca tgaccgccgt cggagtcgtg
     2161 accggccgag cccgcgtggc gtgcggcctc gccgtgctcg ccccaacgcg ccccgtggcc
     2221 cgcccgatgc cggtcgtgca tcgcggtgta gggcgacgga gtctgcgagc cggcgccgac
     2281 cggagccgag gtcgcggccg ctgccggcac tcgttcgctc ctcgccccct ccttgaccgc
     2341 gaccgcgccg ccgccgatga cgacggccgc ggcggcggct gtgaccgccg ccttggtcgt
     2401 gacggcggcg ccgatcttgg ccagcagccc ggcgccggcg gggccggcgg ccgaggcgat
     2461 gtcgggtgaa ggcagcgcgt gcacgggcgc gaggcccgca ggcagcggcg gcatcgccag
     2521 cagcgcggcc ggcacgctga ggggcatgag cacgaggccg agcgcggccg gggcggcagc
     2581 atccgcaccc gcgagcttgg cgaacacggt cttgaacgac gcgcgcgccc ggtgcacgag
     2641 tacgtcggcg ttcggccgcg agatctccat gacctcggcg atctcctcgg gcggcaggcc
     2701 ctggaggtcc ttgagcacga gggcggtgcg gtagcgctcg ttcatctcgc cgagcgactg
     2761 ctccaccagg gccaccgtcc gggcgcgctc gaactcatcg acgccggagg cggcctgctc
     2821 gaggccggcg tcgccgccgc ggtcgagctt gcgccgcgag cgcaggtggt tgaagcaggc
     2881 gttggtggcg acgcggtaga gccaggcgcg caggcggaac gactcctcgt cctcctgcgg
     2941 cagctggtcg aacgccttga tgaacacttc ctgggtcacg tccttggcct cctcgcggtc
     3001 gccgagcacc cgagcgcaga ggttgtagat gggtgcgtgg tactcgccgt agaggcgctc
     3061 gaaggcgtcg cggcgcccgg cccggaggcc gcgcacgaga tcgggggctg tggcgggggg
     3121 tgaggacatc agaggagttc cgcgctcaac ggtactctca tcctgccggt cttcaaccgt
     3181 gcccgccgca ccgcggccgg ccgttgccgg ccggccgctg tgacttgcgt cgtgatcgtt
     3241 cagtcaccgc cgtggcctcc accgtggccg ccgtcgtggc cgccggacga ggcgtgcgtc
     3301 tgctggctgg tctcgtagtg gtgcgagccg gacgggctgg cggagccgct gtgatcgctc
     3361 tgctcgctct gaacgctgtt ccggacgcgc tcgacgtgcc gagcctcggc ctcatgggcg
     3421 ccctgttgcg cctgggcgtg cttgcgctcg acgcggcgcg cgtgggtctt ggtgcgctgc
     3481 gcctcgcgga cgtgactgcc ctggtggtcg atcagccggg ccagctgctt gtccgtgagc
     3541 gtcaccgtgt gctgggcgcg cgtcttgtcg gctgcctgat cgtcctgcgc cttggtgagc
     3601 tgctgcacgc ccgccatggc gacgctcgtc atcgcgaaca cgacgaccgc cgtggcgacg
     3661 atggtcttga gtaccttcat ggtctctccc tgggttcgtt gtcctttcac ttgcttgaac
     3721 acggggaggc ggcgtttctt acacaggttg cgcaggaaca tcgagcgcct gcccgggccg
     3781 gcgtggtcgc gcctcacgct ggagagcgac gacagggtgt acacgccctc cgacctgctg
     3841 cccgtctttg ccgacaccgg cgttgccgct ggtctacgaa gtgcatcacc accgctgcct
     3901 gccgatggcc tcagcgtggc cgagatcgac cggtgcgcgc ggaagacatg gccgagccgc
     3961 cgtttcggct tggaagtcga ggccaaagcg aaggaactgg cggtggccag actgatcgcc
     4021 gatccggatg gcgaaggagc gcgtcgcgag tcgggttgag ccgcgcctac gaagagctcg
     4081 gaccggcgcc cgggaggaga agctcggcga gactcgcggc gagccagtcg cggtagcggc
     4141 gctgcgacca gccgcgcagg cgcacgagga gccggtgcag ctccgggctg gtcagcgccc
     4201 agaccgtctc ggcagcctcc tgctcgctga cgcggagggg tccgttggcg acgtcgtccg
     4261 cgtcgagggc ggtcgcatcg tcgagcactg gggcgtgccc gaccgactca ccaccctctt
     4321 ccagctcggg ctcgcacagc cgccgagcgc cgcgccttca agcgcggcgc ccggcgtggg
     4381 tcctctgccc gcgaagaatc gcgcgcccga gctcactccc gactgacgat atcgaccgag
     4441 atctccaccg ccgggatcgt acctctgttc tcgagccagt gtgtcgtggt ccggtcctcg
     4501 ggccagccca ctcccggccc atagtccgtg gcggttccgt ctcgatgatc cgtgatcgtc
     4561 ccttgcagga tgtagacggt gcctggtctg cccttgtggt cgtgcatcgg gccgaagacg
     4621 cctccaggct ggatggtcac catacgcatg cgaagccggc gccctgccat gccctcgatc
     4681 tcagggccaa ggtcgaccgc tgccagtgac tccaccgtga cacctttcgt ctcaggtgcc
     4741 atctcttcat cgctcatcgt gctccctctc ctgcgatcgg cttcacttct caacgatctt
     4801 ccaatacgag tccttcctga tgaccttgcc gccgcggaac tcccagtgat cacatccacg
     4861 gacctggact ttggtcccat cgggtctcgt ccctgtcagg agccactccg agacacccag
     4921 atcgccactc acccagtggc ggtcatcgtt gtagtggaca tcggggagcc ccgcgaatcg
     4981 cgacgcaact ccctcgcgca ccgccacctt gccgacgaag cgttgccccc aagcgtccgg
     5041 ccctcgaggc aggtccatcg agcagtcctc cgcgaagaac tccatgattg cgtcgagatc
     5101 gtggcgattg aaggcgtcgg cgatcatctt cagcgtctcg accgtgacgg atcccggatc
     5161 agccatcgcc tttgcctccg tctcgtcgtg ggtgcttcaa gacagctgcc tgacgaccgt
     5221 ggccttgagc ggcgcggcca gcaactccgc cagaggcccg tagactccac tggccatcac
     5281 cgcctgcacg gccgtcgtcc gagcctcggc cgtctcccaa tcggcgatct cgatcagcgc
     5341 atcggggtcc tcgagtaccg cgtagcgggt gctgcccaga tagccgggcg cgctcgggcc
     5401 gctcttcgca tcgacgctct cgagcagact tctgagctct tcgcgcctgc ctggtctcgc
     5461 gtgaaattcg atgatcactc tgatagccat ctcggattct cgcctcggtc ggaacggcag
     5521 accgcgccct ctttcaggcg gcctgacggc gctgcagcat ctcggcgagg cccggggagg
     5581 tgtccgtgtg atggtggacc atcttccact ggccgtcctc gaggcggtag acgttcgtca
     5641 cgcgatgctc gaactcgatc ggctctccgg cgagcctggc actgccacgc tcgatgccgg
     5701 tctcgaacgc caggtccgtg cccgcgtaga ccctctggtc gacgagctcc acgtggctgt
     5761 cggtcatcgg tcccgcggcc tgctcgaacg aggcgcggac ttcttcccag ccgacctgct
     5821 ctccgcccat ggggtgcatc gtcgtgacgt catcgctgtg ggaccagatc tcgggcaagg
     5881 ccgcgacgtc catgcggctc agccccgtgt agaacctgtc cgatatcgct cggacatcgt
     5941 cttccctgga catgactcgt cctcctgtca gcagtggcct cgaagccgcc acattcgatc
     6001 catgggctgt agatgccccg cggtgcatcg ctgacacggc cggctgttgg ggcgttcgtg
     6061 tcgccggccg atggatgccg gagcgcggtg ggttgcaggc gctcggggac gggtaggaga
     6121 gtggtgcgct agtcccagta cgactatcgt ccgcctgcgc cgtccgaccc gagcctcatg
     6181 cacctgagcc agggacccat gggtcccagt gcgctccccc tgcgagcgtc gcattcccga
     6241 tcaccatgtt gatggggcga ccgtcgggac cagagcgctc gcgaaggcgc gaagtcgggg
     6301 tcgactacct cgtgacggcg caccactcag catccagcct gcgccgcgac gcaggcgaag
     6361 gaggcagcag taatgccgaa ggtctcacaa gacagtgcgc cacaacgtca ggaagctgga
     6421 ccggtggtgg atctgcgcga cgagctcgac ggctacaccg tgagcttcac gtcgctcctg
     6481 caggatatcg acgcgacgcc cttcatgaag gggcttccgg acgaccgctg ccagtgcccg
     6541 cactggggct acgtcttcaa gggcaagatg accgcgcggt acgccgacca tgacgaggtc
     6601 ttcgaggccg gcgacgcctt ctacatcccg cccggccaca tcccggtcga gaacgagccc
     6661 ggcaccgagt tcctgtggtt cagccccagc gaagagctgc gcacagccga ggcggcgatg
     6721 atgaagaaca tgcaggcgat gcaggacggc tcaacgtctt aggcgaggag gcgcgcgggc
     6781 acccctcctt gggtagcagg ccgtcacctc cgtggcttct tgtacccgcg cgcgacgggg
     6841 ccgctggaca agtgtccagc ggccccgtcg acgagcggag cgcccggcgg cgttctctcg
     6901 cgtcgcggcg cctaagccct cacggcagtg cgcacgccgg ctgaccgggg gcgccacact
     6961 ccaacgccgg ccgcacggcc ggcttcgtga tccgcacgcc gatcacgagc aggtagccga
     7021 ccatccagac ctcggcgata gccgccggga tgacgacgta ggcggagatc gcctcgccga
     7081 agccggggac caggaacagg gcgagtgtgt ccacgaggta gctcgctcgc gtggtcgcag
     7141 gcggacgagc aggcgcgtac catcgggtca gcatgtcgac tccagctgcc tcctcgcctt
     7201 ccagaccgtc cgaactggcc gttggggact tccccgctca aggggcgatc ctgcgcgtcg
     7261 gcggcatggc gttcgcaaac ggcgtcctca tgcgctccgg tagctcgtgg gcgtgggcgc
     7321 gcgacgatgg gaccgtgctc cacgggacag tcgcgtcgtt gctcgagggc cggcgctggc
     7381 tgcgactccc ggtgctgcgc tcggccgtct ccttcatcga gatgactgcg ctgagcctca
     7441 gattgcatcg cctcaacggc ggccggcgcg gtgtgaggct gatcgtctgt ctggtcatgt
     7501 gcgtgctggc aggcatcgcc ctgggctccg ccgtccacgc cttggtcggc agtcagatgc
     7561 tcagcggcct cgtgatgcag gcgtcaggca tgctgttggc gctcgcggtc ctgcaggtgg
     7621 ggatgggcgc ggaggtctgg cgctaccacg gtgccgagca caaggtcgtc aatgcctacg
     7681 aggcgcgggt cgacctgcgc gatgtggcgg ccgtgaagcg cttcagccgg atccacgatc
     7741 gctgcggcac gaacctcgtg gcgatcgtct tcgcgctggc gctcctgtgc ttgccgatgg
     7801 gcaccagcgc cctcgcgcag gcgctctccc tggtcacggc ggtcctggtg atcgccgtcg
     7861 cactggagct gttccgtctg atcgaacgct cgccgcacgc gcgcttcagc cgtgtgctgc
     7921 tgtcgggcgg caggaccctg cagcggctgc tgacgacccg cgagccgcgt gcggagcacc
     7981 tgcacctcgc cagcagggcc ctgctcgagg tcatcgaggc cgagtccgcg aagtcccagg
     8041 ccgcgccggc ctcgtgagcg tgaggccggc gggctcgcgc caagcacgcc gctacgccct
     8101 cagcgacccg tccacaccgg cgccgtccag gcggtcacgc cctcgttgcc ccagaagtcg
     8161 gaggcggtcg tgacgcgcac caggaagtag cgctcctctt tcgaggtcac cacggtcgtc
     8221 cactcggcct cggcctcatc gaacgccttg ctcgcgacga cctcgccgcc gtcggccacg
     8281 atctcgacca gcttgacctc gtcgccggcc ccgtccgggt ccttgatctc tatcttggcc
     8341 tcgtacgagg ccttcggcgc caggtcggag cccatgacgg ccccgtcgag cgtgtagcgg
     8401 acctcgaggt cctcgtccat cgtcgcgtag ccgcggctcg ctcgcagcgc gccgagcagc
     8461 ccctcccgag tgagatcctc ggagagcagc accgtccgca cgtcacagcc cgtgatccag
     8521 tccgcggcgt gcgtgtccga gttggcgctc ggcagcacgt gccagccggc gtcgagcgcc
     8581 tcgaagtact cgtcctcgta gatctcctcg ttccagacct cgaccatggt gacgccctcg
     8641 tcgcgctgcg gcgtccacca gtcgaagtgg aagaagtcga cgagggacga gccgccctgc
     8701 gcgccgtaga gaccggggtg gttccactgg ccgacggccc cggtctcgcc cagccaggcg
     8761 tacaggtcgg gcagcacccg gcggatgctc tgcctgatgc cgttgttgta gccgcggtcg
     8821 cccgggttgc ccgactgtcc gaacatctcg gtcgtgttga agacgttcat ctcaccgacg
     8881 gccggcagcc acagttcgta gccggcgaag gcgacgaagg tctcgtcggt gaaggcctcg
     8941 ctcagcgcga cggtctcggc gtagcgcgag ggcgtctgcc agccggtgcc gtagccgtag
     9001 tgggtgtggt cggtgagggc gaagaagtcg gcgcccgctg ccttcgccgc cgtgtaggcc
     9061 tgccacggtg tgcccgtgcc gtcggagtag tcggtgtgcg cgtgcatgtc gccgaagtag
     9121 aggtcgtagg gcgctgcgcc ggccgccgcg ccggccgccg cggtcgcgac cagcccgaac
     9181 gcaaggacca gtgaggtgca gagcagtgtc gcaacgaggt gccgcttcat ggtgtcccct
     9241 ttggtcgcgt gaccttctcc ccgctcctga atgcagcata gccccggggt ccgcaacggg
     9301 gaaggagggc gcgtttgccg tctgttcgcg ttcgggtcgt cgatgaggca ggcgcgagaa
     9361 cggggagggc ctttggccga gtggtaggct cttcgtgctc gaaggggtcg atcgaaaggg
     9421 gggatccctt gcagcgtcgt gccctcaccc tcgctgcggg ccatgtcagg cgaagtcgcc
     9481 tcgtcggctt cgtcggcgcg gtctgcgccg tgctgctcct gaccacgccc gcgcttgccg
     9541 cgtcctccgg gcagaggctc tgggtgcgca cctaccgtcc ggcctccggc ggcgccacct
     9601 tctccgacgt ggcggccgga gcccgtggag cggcctacgt cgccggcagc aagcgcatcg
     9661 accagtcgct tgcgctgatc ctgctcaagt acgacggcgg cggccggcgg acgtgggcgc
     9721 atgcgttccg gggcgactac cgtctgacca cgggcacggc cgtcggcctc gacgcgcgcg
     9781 ggaacgtcta cgtcggcggg gacgtccgct ggcgaaacgg cgagagcggc atcctcctcg
     9841 tgaagtacgc gtctgacggc acgcgcaggt ggtcccgcac ctggaacccg t
//
LOCUS       DASUMX010000085         9921 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1460315, whole genome shotgun sequence.
ACCESSION   DASUMX010000085 DASUMX010000000
VERSION     DASUMX010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 9921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9921
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1460315"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..596
                     /locus_tag="VFZ86_04055"
     CDS             <1..596
                     /locus_tag="VFZ86_04055"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5641498.1"
                     /translation="FGIDVERPLLLQVSRFDPWKDPLGVVDVYRAVKAKHPDVQLALI
                     GSMASDDPEGWDYLERVISYVGGDPDVFILSNLDNVGSVEINAFQSHADVIMQKSTRE
                     GFGLTVTEGLWKGRPVIGGAVGGIPLQIEDGVTGYLVHSAAECAERCLQVMADPARHR
                     EMALRGKEKVRREFLTPRLLRDDLRLFADLESGTIGA"
     gene            619..2130
                     /locus_tag="VFZ86_04060"
     CDS             619..2130
                     /locus_tag="VFZ86_04060"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEX5641499.1"
                     /translation="MEGVDLTQRRLVVAANRGPVSFHDDPSGEPVVTRGPGGLVTVLT
                     EVLRHHPGTWVAAALSDAERRLAAENRAVEVALDGEEYRVRYVAPSPDAYHKYYNVVS
                     NPMLWFIQHYLWDLGRHPDIRAEEMDAWHNGYLVVNEQFARAVADEVRRGPDGRRRRG
                     PAGDGDDALVLLHDYQLYCVAPDVRAACPGAFLHQFVHIPWPQSDYWRVLPTHIREAV
                     FRGLLGNDVVAFHTRRYVRNFLRCCADLLDLEVDEARSVVRHEGREVWVRAYPVSIDP
                     ESLRAAAGSRRARTAERKLRGRRREFLLLRVDRLDLSKNIIRGFVALDRFLELHPEFK
                     ERFTFLALLQPSREDVEEYVTYRERVERLVADVNTRHGTTDWMPIELRIQDDFPTTLA
                     AYAQYDVLLVNAIADGMNLVAKEGPILNRHDGVLVLSEYAGAYEELGAFALGVNPFNI
                     ESQAEAIHRALVMPPDERASRAAMLRRVVEENSVEKWVQAQFADIAAKLAAGA"
     gene            2175..2453
                     /locus_tag="VFZ86_04065"
     CDS             2175..2453
                     /locus_tag="VFZ86_04065"
                     /inference="COORDINATES: protein motif:HMM:NF024590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX5641500.1"
                     /translation="MRIDVLGLPGETTAALEAALRSALDELRLDGVVVGRISDPGLMI
                     ARGVRKPPALMIDGRVVCRGRVPSADELRDLLAAARSGASGDQPMRCG"
     gene            complement(2432..3142)
                     /locus_tag="VFZ86_04070"
     CDS             complement(2432..3142)
                     /locus_tag="VFZ86_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641501.1"
                     /translation="MPEGNINAEVADHLREHGAHDEHGEQHAPSKRRMETIEILEAVL
                     LAFVAILTALSGYQAARWDGESARAYAESNRLRVEANEKHLEANQTLAYDAGTLNAWL
                     QAYVAGDEELQDVLERRFTPEFEEAFQAWIALDPLENPDAPAGPGSMPEYENTLLEEA
                     DHLTEEASHAFEEGVETRETGEHYVRLTVILAAVLFLVAIGQRFTIKRVRHTLMWVAG
                     AFIVYCIVLFATYPHLMG"
     gene            complement(3218..4312)
                     /locus_tag="VFZ86_04075"
     CDS             complement(3218..4312)
                     /locus_tag="VFZ86_04075"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEX5641502.1"
                     /translation="MTNAGAGTDGHAGAGEDLAAYLTAGGEPADRPEPLFLLGDAAET
                     LRLFPAASVDCVVTSPPYFGHRRYAGGGIGAEGGWRDYVAALGAVTAELPRVLKPTGS
                     LWLNLGDTYRGKRQLGIPWRVAFHLTDAQGWTLRNSVVWHKVKGGPDTSRDRLRNVHE
                     LLFHFVRQPKGYYYDADAVRRAPRAARVEDGAVVSGTGVRGVRYRRQIELSTSLDALE
                     KAAAIAALDDTLAEVEAGRLGDFRMIIRGRQRATHSDVGAVSGRARELEERGFCVLRY
                     HPGGAKPGDVWDILPEDTQGRGSHFAPFPADLCRIPILATCPPDGVVLDPFCGTGTAM
                     LVASRLGRRSVGIDLAAEYLALAGERLRER"
     gene            complement(4309..5334)
                     /locus_tag="VFZ86_04080"
     CDS             complement(4309..5334)
                     /locus_tag="VFZ86_04080"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX5641503.1"
                     /translation="MRIAQLAPPWITVPPAGYGGTEWVVQQLCDGLVASGHEVRLYAT
                     GDSHTAAELCALFPEQMPDRIGVTPYDARQVSFAFADIEASGFDLVHDHSGFLGVAFS
                     RYLATPMVHTVHCAFDEAAFGFYEQFAGDVGYICISEYQRSMAPPGMDWAGRAYNAIA
                     VEQWPFTPEKDDYLLAFGRVCEAKGFHHSIEAAKRLDRRLIMAGVLQEPYREYFEEKV
                     LPHIDGDQIVYEGEVSDQRKRELFAHAHAFLFPITWPEPFGLVMIEAMACGTPVVALR
                     QGSVPEVVDDGVTGFVVDGFEEFVAAVGRVGEIDPAVCRRTVEQRFTVERMVTDYEAI
                     YRRYLDR"
     gene            5510..6793
                     /locus_tag="VFZ86_04085"
     CDS             5510..6793
                     /locus_tag="VFZ86_04085"
                     /inference="COORDINATES: protein motif:HMM:NF014072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LarC family nickel insertion protein"
                     /protein_id="HEX5641504.1"
                     /translation="MILYLDCSSGISGDMLVGALLSLAGARESDPGPLDDVLRPALAA
                     AGIDPRLVQIEDARRGSLAALAFRVAEQPGFATFDEMIVSLYASDLEQPVTDAVASIA
                     ERMAAAEAEVHGRDEAEHLHELSDVDTAVDLICAVTLLAKLAPERVVASPPALGDGEV
                     ETAHGALSVPAPAVLSLLAGLPTAGGAPPVAAGETPAAPLGELTTPTGAALLAHFAGE
                     YGGLPAGRIERIGYGAGRRELPGRPNVLRAVLVDPAGLSDGAATGPEADAAALAAQGG
                     HGRGEHVVLEANIDDMTPELLAHAANALREAGALDVWMSEALMKKGRPGVVLHVLAPA
                     ADRERLAEAVFAETTTFGLRVVACAREYVEERRESVKIGKLKVNVRLSFVRGRLATVS
                     PEYDDVRRVATAVGRPARVVYEAVQSAARSRFSGS"
     gene            7012..8721
                     /locus_tag="VFZ86_04090"
     CDS             7012..8721
                     /locus_tag="VFZ86_04090"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEX5641505.1"
                     /translation="MSSIVRKSPVPSDIEIAQAATMQPIQAIAEKAGILEDELELYGR
                     YKAKIDYMAVLERLKDKPDGKIINVTAITPTPLGEGKTVTAVGLTESMNAIGMDTMLA
                     LREPSLGPVFGIKGGAAGGGYAQLLPMEDLNLHFTGDIHAVGTANNLLAAMVDSHLMH
                     GNELDIDPLTISWRRCVDLNDRAMREIVNGLGGRLNGLPRQTGYDITVASEVMAILGL
                     ATSLKDLRERLGRITFGYSKTGKPLTADDIGAGGAMTVLLKDALKPNLMQTLEGNAAL
                     IHAGPFANIAQGNNSIIADKVAMKLAEYVVTESGFAADLGSEKFMDIICRYGGFAPSA
                     VVITCTMRALKMHGGLPVDPELLKQENNEALTKGCENLAKQIENMKYFGLPVVVTINR
                     FAFDRDSEVEIVRKEAVAAGAEDAVPINVHALGGDGGREAAEVVKAAADKGGDFKFLY
                     PLEASIKEKIETIATKIYGADGVDYLPEAEAKIKQFTEQGLDKLPLCMAKTHLSLSHD
                     PALKGRPRGFRVPIRDIRPATGAGYLYPLLGEMRTMPGLAKRPAAVDVDIDVETGRII
                     GLF"
     gene            8864..9505
                     /locus_tag="VFZ86_04095"
     CDS             8864..9505
                     /locus_tag="VFZ86_04095"
                     /inference="COORDINATES: protein motif:HMM:NF016825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HEX5641506.1"
                     /translation="MSDGPTIYATEPLQVYLDDAASKKPAPGGGSVSAAVGALGAALV
                     SMVCNLTQGREKFADVETEIVALVGQADDDRRRLEQLLQDDTTAYNGVIDAFKMPKET
                     DAEKAARKAAVQAGLIIAADVPLEICRVAVDVCRLSKVAAQLGNPQAVTDAGIGAILG
                     EAAVVGAALNVRINLGSIEDCDYTIAAAAEIDKIQAEAAALREEVLVITLSKL"
     gene            9541..>9921
                     /locus_tag="VFZ86_04100"
     CDS             9541..>9921
                     /locus_tag="VFZ86_04100"
                     /inference="COORDINATES: protein motif:HMM:NF012965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HEX5641507.1"
                     /translation="MAAQIIDGKAIAEQVREEIKAEVARLATCGVKPGVATILVGEDG
                     GAQFYRGQIEKNTATVGFSYFNNTLPAGTSEAEILDLIAKLNDDPRVHGILALMPMAG
                     GIDQNAVFNAIAPVKDLDCVNPANI"
BASE COUNT         1500 a         3603 c         3460 g         1358 t
ORIGIN      
        1 agttcggcat cgacgtggag cgcccgctcc tgctgcaggt ctcccgcttc gacccctgga
       61 aggacccgct gggggtggtc gacgtctacc gcgccgtcaa ggcgaagcac ccggacgtgc
      121 agctcgcact catcggctcc atggccagcg acgacccgga gggctgggac tacctcgagc
      181 gcgtcatctc ctacgtgggc ggcgacccgg acgtgttcat cctctccaac ctcgacaacg
      241 tcggctccgt ggagatcaac gcgttccaga gtcacgccga cgtgatcatg cagaagtcca
      301 cgcgcgaggg cttcgggctc accgtgaccg agggcctgtg gaaggggcgc cccgtgatcg
      361 gcggcgccgt cggcggcatc ccgctgcaga tcgaggacgg cgtgaccggg tacctggtcc
      421 actccgccgc cgagtgcgcc gagcgctgcc tgcaggtcat ggccgacccg gcgaggcacc
      481 gcgagatggc gctgcgcggc aaggagaagg tccgccgcga gttcctcacg ccgcgcctgc
      541 tgcgcgacga cctgcgcctc ttcgccgatc tcgaaagcgg tacgataggg gcctgaccag
      601 cgacgcaggg ggcccggcgt ggagggtgtc gatctcacac agcgccgtct cgtggtggcg
      661 gccaaccgcg gccccgtctc gttccacgac gacccgtcgg gagagcccgt ggtcacgcgc
      721 gggcccggcg gcctcgtcac cgtgctcacc gaggtgctcc gccaccaccc cggcacgtgg
      781 gtggcggcgg cgctgagcga cgccgagcgg cgcctggccg ccgagaaccg cgccgtcgag
      841 gtggccctcg acggcgagga gtaccgggtc cgctacgtcg ccccgtcccc ggacgcctac
      901 cacaagtact acaacgtcgt gagcaacccg atgctgtggt tcatccagca ctacctctgg
      961 gacctcggcc gccatccgga catccgcgcc gaggagatgg acgcctggca caacggctac
     1021 ctcgtggtca acgagcagtt cgccagggcc gtggccgacg aggtgcgccg ggggcccgac
     1081 gggcgccggc gccgcggccc ggccggcgac ggtgacgacg ccctggtgct gctgcacgac
     1141 taccagctct actgcgtcgc cccggacgtc cgcgccgcct gccccggcgc cttcctgcac
     1201 cagttcgtcc acatcccgtg gccgcagagc gactactggc gcgtgctccc cacccacatc
     1261 cgcgaggccg tgttccgagg cctgctgggc aacgacgtgg tggccttcca cacgcgccgc
     1321 tacgtgcgca acttcctgcg ctgctgcgcc gacctgctcg atctcgaggt ggacgaggcg
     1381 cgcagcgtcg tccgccacga gggccgcgag gtctgggtgc gcgcctaccc ggtgagcatc
     1441 gaccccgagt ctctgcgcgc ggcggccggg tcgcgacggg cgcgcaccgc cgagcgcaag
     1501 ctgcgcggcc ggcgacggga gttcctcctg ctgcgcgtgg acaggctcga cctgagcaag
     1561 aacatcatcc gcggcttcgt cgccctcgac cgtttcctcg agctgcaccc ggagttcaag
     1621 gagcggttca cgttcctcgc gctcctccag ccgtcgcgtg aggacgtgga ggaatacgtc
     1681 acctaccgtg agcgggtgga gcggctcgtc gccgacgtca acacgcgcca cggcaccacc
     1741 gactggatgc ccatcgaact gcgcatccag gacgactttc cgaccacgct ggccgcctac
     1801 gcgcagtacg acgtcctgct cgtcaacgcc atcgccgacg ggatgaacct ggtcgccaag
     1861 gagggcccga tcctcaaccg ccacgacggc gtgctggtcc tgagcgagta cgcgggggcc
     1921 tacgaggagc tgggcgcctt cgccctcggc gtgaacccgt tcaacatcga gtcccaggcg
     1981 gaggcgatcc accgggcgct cgtcatgccg cccgacgagc gtgcgtcgcg ggcggccatg
     2041 ctgcgccgcg tcgtcgagga gaacagcgtc gagaagtggg tgcaggcgca gttcgccgac
     2101 atcgccgcga agctggccgc cggcgcttga cccaggtcat ggcgccggcg ctcgtcctcc
     2161 ggcagcatgg cgtcatgagg atcgatgtcc tgggccttcc cggtgagacc acagctgccc
     2221 tcgaggccgc gcttcgctcg gcactcgacg agctccgcct cgacggcgtc gtcgtgggca
     2281 ggatcagcga cccgggcctg atgatcgctc gcggcgtccg gaagccccct gcgctcatga
     2341 tcgacggcag ggtcgtgtgc cgcgggcggg tgccgtcggc ggacgagctc cgcgatctgc
     2401 tggcggcggc gcggtcaggc gcgtccggcg atcagcccat gaggtgcggg taggtcgcga
     2461 acagcacgat gcagtagacg atgaacgcgc ccgccaccca catcagggtg tgccggaccc
     2521 tcttgatggt gaagcgctgg ccgatcgcga ccaggaacag gacggcggcc aggatcaccg
     2581 tcagccgcac gtagtgctca ccggtctccc tggtctccac accctcttcg aaggcgtggg
     2641 acgcctcttc cgtgaggtga tcggcttcct cgaggagcgt gttctcgtac tccggcatcg
     2701 atcccggacc ggcgggcgcg tcgggattct cgagcgggtc cagcgcgatc caggcctgga
     2761 aggcctcctc gaactccggc gtgaaccggc gctccagcac gtcctggagt tcctcgtcgc
     2821 ccgccacgta cgcctggagc cacgcgttga gcgtccccgc gtcgtaggcg agcgtctggt
     2881 tggcctcgag gtgtttctcg ttggcctcca cgcgcaaccg gttggactct gcgtacgcgc
     2941 gggcgctctc gccgtcccag cgcgccgcct ggtagccgct gagcgccgtg aggatcgcca
     3001 cgaaggcgag caggacggcc tcgaggatct cgatggtctc catgcggcgc ttgctcggcg
     3061 cgtgctgctc gccgtgctcg tcgtgggcac cgtgttcgcg cagatggtcc gcgacctcgg
     3121 cgttgatgtt gccctccggc atggtgcgac tcctcccgct gctcgtcgtt gccgacggca
     3181 gatgctacta caggagctga ccggacggta accccggtca gcgctcgcgg aggcgctcgc
     3241 cggcgagggc gagatactcc gccgcgaggt cgatgccgac ggagcggcgg ccgagccggg
     3301 aggcgaccag catcgccgtc cccgtgccgc agaacgggtc gaggacgacg ccgtccggcg
     3361 ggcacgtggc caggatgggg atgcggcaga ggtcggccgg gaacggggcg aagtgcgaac
     3421 cgcgtccctg cgtgtcctcg ggcaggatgt cccagacgtc gccgggcttg gcgcctcccg
     3481 ggtgatagcg cagcacgcag aagccgcgct cctcgagttc gcgagcgcgg cccgacaccg
     3541 cccccacgtc cgagtgcgtc gcccgctggc ggccgcggat gatcatgcgg aagtcgccga
     3601 ggcggccggc ctccacctcg gcgagcgtgt catcgagcgc ggcgatggcc gccgccttct
     3661 cgagcgcgtc cagtgacgtc gagagctcga tctggcggcg gtagcgcacg ccgcgcacgc
     3721 cggtcccgga gacgacggcg ccgtcctcca cgcgcgccgc gcgcggcgcg cgcctgacgg
     3781 cgtcggcgtc gtagtagtag cccttcggct gccggacgaa gtggaacagc agttcgtgca
     3841 cgttgcgcag cctgtcgcgg ctcgtgtccg gcccgccctt gaccttgtgc cacacgacgc
     3901 tgttgcgcag cgtccagccc tgcgcgtcgg tgaggtggaa ggcgacgcgc caggggatgc
     3961 cgagctggcg cttgccgcgg taggtgtcgc cgaggttgag ccacagggag cccgtgggct
     4021 tgagcacgcg cggcagctcg gcggtgacgg cgccgagggc ggccacgtag tctcgccagc
     4081 cgccctcggc gccgatgccg ccgcccgcgt agcggcggtg gccgaagtag ggcgggctgg
     4141 tgacgacgca gtcgacggag gcggcgggga agaggcgcag cgtctcggcg gcgtcgccca
     4201 gcaggaagag cggctcggga cggtccgccg gctcgccgcc ggccgtgagg tacgcggcga
     4261 ggtcctcgcc cgcgccggcg tggccgtcgg tccccgcgcc ggcgttcgtc acctgtccag
     4321 gtagcgccgg tagatcgcct cgtagtcggt gaccatgcgc tcgacggtga accgctgctc
     4381 cacggtgcgg cggcagaccg ccgggtcgat ctcgccgacc cggcccaccg ccgccacgaa
     4441 ctcctcgaac ccgtccacga cgaagccggt cacgccgtcg tccacgacct ccggcaccga
     4501 gccctggcgc agcgccacca cgggggtgcc gcacgccatg gcctcgatca tgaccaggcc
     4561 gaacggctcg ggccaggtga tggggaagag gaacgcgtgc gcgtgggcga agagctcgcg
     4621 cttgcgctgg tcgctgacct cgccctcgta gacgatctgg tcgccgtcga tgtgaggcag
     4681 gaccttctcc tcgaagtact cgcggtacgg ctcctgcagc acgccggcca tgatgagcct
     4741 gcggtccagc cgcttggccg cctcgatgct gtggtggaag cccttggcct cgcagacgcg
     4801 gccgaaggcc agcaggtagt cgtccttctc cggcgtgaac ggccactgct ccacggcgat
     4861 ggcgttgtag gcgcggccgg cccagtccat gccgggcggc gccatggagc gctggtactc
     4921 gctgatgcag atgtagccga cgtcgccggc gaactgctcg tagaagccga aggccgcctc
     4981 gtcgaaggcg cagtggacgg tgtggaccat gggcgtcgcc aggtagcggc tgaaggcgac
     5041 gccgaggaag cccgagtggt cgtgcacgag gtcgaacccg ctcgcttcga tgtcggcgaa
     5101 cgcgaacgag acctggcggg cgtcgtaggg ggtcacgccg atcctgtcgg gcatctgctc
     5161 ggggaacagc gcgcacagtt ccgccgcggt gtgcgagtcg ccggtggcgt acaggcggac
     5221 ctcgtgaccg gacgcgacga ggccgtcgca gagctgctgc acgacccact cggtaccgcc
     5281 gtagccggcg ggcgggacgg tgatccacgg cggtgcgagc tgggcgatgc gcatggcggc
     5341 attctacagg acggaggcgc ctcggccaga gtgcagcgcg gacgggccgg tctcgcgggg
     5401 gctcatgcgc cgccgccgac gtgccgatgg gaggcgcaca cccggcgtcc ggctgcgccg
     5461 gcccgggcag agtcaccctc agacgtgacg acgaggtaga gtgacggcga tgatcctgta
     5521 tctggactgc tcgagcggca tcagcggcga catgctcgtg ggcgcgctgc tgtcgctggc
     5581 cggggcgcgc gagtcagacc ccggaccgct ggacgacgtg ctgcgtccgg ctctcgccgc
     5641 ggccggcatc gaccctcggc tggtgcagat cgaggacgcg cgccgcggca gcctggcggc
     5701 gctcgcgttc agggtcgccg agcagccggg cttcgcgacc ttcgacgaga tgatcgtgtc
     5761 gctgtacgcc tcggacctcg agcagccggt caccgacgcc gtcgcctcca tcgccgagcg
     5821 catggccgcg gccgaggccg aggtgcacgg gcgcgacgag gcggagcatc tccacgagct
     5881 gagcgacgtc gacaccgccg tggacctgat ctgcgccgtg acgctgctgg cgaagctcgc
     5941 gccggagcgc gtcgtcgcct caccgccggc gctcggcgac ggcgaggtcg agacggcgca
     6001 cggtgcgctg agcgtgccgg cgcctgccgt gctctcgctg ctggcgggtc tgcccacggc
     6061 cggcggcgcc ccgcccgtgg cggcggggga gacgccggcc gcaccgctcg gcgagctgac
     6121 gacgcccacc ggcgccgccc tgctcgcgca cttcgccggg gagtacgggg gcctgcccgc
     6181 ggggcggatc gagcggatcg gctacggcgc cggccgccgc gagctgcccg gccggcccaa
     6241 cgtgctgcgg gccgtcctgg tcgacccggc gggcctgtcg gacggcgccg ccaccggccc
     6301 ggaagccgac gccgcggctc tcgccgccca gggcggtcac ggccgcggcg agcacgtcgt
     6361 cctcgaggcc aacatcgacg acatgacgcc cgagctgctc gcgcacgccg ccaacgcgct
     6421 gcgcgaggcc ggtgcgctcg acgtgtggat gagcgaggcg ctcatgaaga agggccgccc
     6481 cggcgtggtc ctccacgtgc tcgccccggc ggccgaccgc gagcgcctgg ccgaggccgt
     6541 cttcgccgag accaccacgt tcggcctgcg cgtggtggcg tgcgcgcgtg agtacgtcga
     6601 ggagcgtcgc gagagcgtca agatcggcaa gctcaaggtg aacgtccgcc tcagcttcgt
     6661 gcgcggacgc ctggccacgg tgtcgccgga gtacgacgac gtgcggcgcg tcgccaccgc
     6721 cgtggggcgg ccggcccgcg tggtctacga ggcggtccag tcggccgccc gcagccgctt
     6781 cagcggctcc tgacggcggc cgccccgccg catcggccgg acgccgcggc cgcgccctcc
     6841 gccctccacc ggcgtcatct ccgtccaccc ctccggacag accgtgttcg aaattgaccg
     6901 tttttgtgcc gcgctagtag aatcgggcac aagctgccgg ctttgccccc gcgggcgctc
     6961 cggccgtcac caacgagacg cgcgggacag tggagaccaa aggaggacgc gttgagttcg
     7021 atcgttcgta aatccccggt gccgagcgac atcgagatcg cccaggccgc caccatgcag
     7081 cccatccagg cgatcgccga gaaggccggc atcctcgagg acgagctgga gctctacggt
     7141 cgttacaagg ccaagatcga ctacatggcc gtcctcgagc gcctcaagga caagccggac
     7201 ggcaagatca tcaacgtgac ggcgatcacg ccgacgccgc tgggcgaggg caagacggtc
     7261 accgccgtcg gcctcaccga gtcgatgaac gccatcggca tggacacgat gctcgccctg
     7321 cgcgagcctt ccctcggccc ggtcttcggc atcaagggcg gcgccgccgg cggcggctac
     7381 gcgcagctcc tcccgatgga ggacctcaac ctgcacttca ccggcgacat ccacgccgtc
     7441 ggcacggcca acaacctgct cgccgccatg gtcgacagcc acctcatgca cggcaacgag
     7501 ctcgacatcg acccgctcac gatcagctgg cgacgctgcg tcgacctcaa cgaccgcgcc
     7561 atgcgcgaga tcgtcaatgg tctcggcggc cggctcaacg gcctcccgcg ccagaccggg
     7621 tacgacatca ccgtcgcctc cgaggtcatg gccatcctcg gcctcgccac gtcgctcaag
     7681 gacctgcgcg agcgcctcgg ccgcatcacc ttcggctaca gcaagacggg caagccgctg
     7741 accgccgacg acatcggcgc cggcggcgcc atgacggtgc tcctcaagga cgccctcaag
     7801 cccaacctca tgcagacgct cgagggcaac gccgcgctca tccacgcggg ccccttcgcc
     7861 aacatcgccc agggcaacaa ctcgatcatc gccgacaagg tcgcgatgaa gctggccgag
     7921 tacgtggtca ccgagagcgg cttcgccgcc gacctcggct ccgagaagtt catggacatc
     7981 atctgccgct acggcgggtt cgcgccgagc gccgtggtca tcacctgcac catgcgcgcc
     8041 ctcaagatgc acggcggcct cccggtcgat cccgagctgc tcaagcagga gaacaacgag
     8101 gccctcacca agggctgcga gaacctcgcc aagcagatcg agaacatgaa gtacttcggc
     8161 ctgcccgtcg tcgtcaccat caaccgcttc gccttcgacc gcgacagcga ggtcgagatc
     8221 gtgcgcaagg aggccgtggc ggccggcgcc gaggacgccg tgccgatcaa cgtccacgcg
     8281 ctcggcggcg acggcggccg cgaggcggcc gaggtcgtca aggcggcggc cgacaagggc
     8341 ggggacttca agttcctcta cccgctcgag gcctccatca aggagaagat cgagaccatc
     8401 gccaccaaga tctacggcgc cgacggcgtg gactacctgc ccgaggccga ggccaagatc
     8461 aagcagttca ccgagcaggg cctcgacaag ctgccgctct gcatggccaa gacccacctg
     8521 tcgctgagcc acgacccggc gctcaagggc cgtccgcgcg gcttccgcgt gccgatccgc
     8581 gacatccgtc ccgccaccgg cgccgggtac ctgtacccgc tgctcggcga gatgcgcacc
     8641 atgcccggcc tggccaagcg gccggccgcc gtcgacgtcg acatcgacgt cgagaccggc
     8701 cggatcatcg gcctgttctg agaacctgaa ggagcgaccc ggggcgccgg ccgttcgccg
     8761 gcgccccgcg gagccctccg gggctccgcg cccggcggcg tccgccgccc cactggtcgc
     8821 tccccgtccg cggcgcgagg acgcgccgcg ctggaggtac acggtgagcg acggtcccac
     8881 catctacgcg actgaacccc tgcaggtcta cctcgacgac gccgccagca agaagccggc
     8941 gccgggcggc ggcagcgtct ccgcggccgt cggcgcgctc ggcgcggcgc tcgtcagcat
     9001 ggtctgcaac ctcacccagg gtcgcgagaa gttcgccgac gtcgagacgg agatcgtcgc
     9061 gctcgtcggt caggccgacg acgaccgcag gcggctcgag cagctgctgc aggacgacac
     9121 cacggcgtac aacggcgtca tcgacgcctt caagatgccc aaagagacgg acgcggagaa
     9181 ggcggcgcgc aaggcggccg tccaggccgg cctcatcatc gccgccgacg tcccgctcga
     9241 gatctgccgc gtcgccgtgg acgtctgccg cctcagcaag gtcgcggccc agctcggcaa
     9301 tccgcaggcc gtcaccgacg cgggcatcgg cgcgatcctc ggtgaggccg ccgtcgtggg
     9361 cgccgcgctc aacgtgcgca tcaacctcgg ctccatcgag gactgcgact acacgatcgc
     9421 cgcggccgcc gagatcgaca agatccaggc ggaggccgcg gcgctgcgcg aggaagtcct
     9481 cgtgatcacg ctctccaagc tctgatcatc accctgacga cgcacacgag agggaaagcc
     9541 atggcagctc agatcatcga cggcaaggcg atcgccgagc aggtccgcga agagatcaag
     9601 gccgaggtcg cgaggctcgc gacctgcggc gtcaagcccg gcgtcgccac catcctggtc
     9661 ggcgaggacg gcggcgcgca gttctaccgc ggccagatcg agaagaacac cgccaccgtg
     9721 ggcttcagct acttcaacaa cacgcttccc gccggcacca gcgaggcgga gatcctcgac
     9781 ctcatcgcca agctcaacga cgacccccgc gtgcacggca tcctggcgct catgccgatg
     9841 gccggcggca tcgaccagaa cgcggtgttc aacgccatcg cgccggtcaa ggacctcgac
     9901 tgcgtcaacc cggccaacat c
//
LOCUS       DASUMX010000086        26844 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_742232, whole genome shotgun sequence.
ACCESSION   DASUMX010000086 DASUMX010000000
VERSION     DASUMX010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 26844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26844
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_742232"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            406..774
                     /locus_tag="VFZ86_04105"
     CDS             406..774
                     /locus_tag="VFZ86_04105"
                     /inference="COORDINATES: protein motif:HMM:NF013334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HEX5641508.1"
                     /translation="MHELGITQGIIDRARDAAVANGARRVAGLHLLMTPAADFTQDSI
                     EMYFEMLASDDELFRGAKLHFEHRPVAATCLSCSDEFTTDAPHPICPHCGSQLVRLDP
                     DAPMVQLTDIDIDEGSDESS"
     gene            complement(787..862)
                     /locus_tag="VFZ86_04110"
     tRNA            complement(787..862)
                     /locus_tag="VFZ86_04110"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(827..829),aa:Ala,seq:cgc)
     gene            complement(921..1781)
                     /locus_tag="VFZ86_04115"
     CDS             complement(921..1781)
                     /locus_tag="VFZ86_04115"
                     /inference="COORDINATES: protein motif:HMM:NF025691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4389 domain-containing protein"
                     /protein_id="HEX5641509.1"
                     /translation="MAPTDYPVDVRADYPERSSRGWAALTIFWIKFFALIPHGIVLIF
                     LGIAQWVVAFVAQFVVAFKGEYPAGMHEFVTGVLRWQTRVTAFVLSVDDRYPPFALKP
                     VDDYPVDVVAERPAQPSRVYAVFTIVVQILGLIALIWMAAWLIDHADTIASWSTDGSY
                     DYQLNLPSWGGGGLLLRQLAAFPHYVVLIFVGIAAFVLWFVVQWVILFGATFPRGMWD
                     LVAGYVRWYTRVTAYALGLVDRYPPFTFDPSLTAVPPAAAAWPAAPPPAPQAASWPEA
                     PPPPAPRQPD"
     gene            complement(1814..2866)
                     /locus_tag="VFZ86_04120"
     CDS             complement(1814..2866)
                     /locus_tag="VFZ86_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641510.1"
                     /translation="MTEPIEFHRRTETAAFYSPLAGMEYAEDRVEVRTDPLTGMTAVA
                     SAGLEAKEEMFLGRADWAYTDQLVARSREGCFFCPENVLEATPRYPDEMLPGGRLERG
                     RALVFPNLFPLAAVHAVVTFPGEHFLRPSQFTPSLLEEGLCAALDFARRAAAFHPRMT
                     HISVCCNHMLPGGASLVHPHFQVFGGETVPWLVQLYWDRSAAWLGARGESYWRTLVEQ
                     ERAAGERYVWGAAGVHWLVPFAPAGAREAIAVVPDAPRLTDLRDEHAAALAHGLTKVL
                     TWYEDEGLSAFNFTLSGGPLDGRDTGFPVVVRVIARTAFKQDYRTDDYFLQKQLGGEL
                     MFAAPEEMAARLRPLF"
     gene            3093..4001
                     /locus_tag="VFZ86_04125"
     CDS             3093..4001
                     /locus_tag="VFZ86_04125"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HEX5641511.1"
                     /translation="MEAEALRKLWGDAGGGGDPAPKGAGLLGLHVVDDPAALTGMALA
                     VLGLPLDDVAGRPAAVRRESLRYADWLPGLSTGFRAADYGDVAVAEEDPALAFMLAHS
                     RLADIVAAGASPLVLGGDALVSVPVLQVLSGKLRGRLGIVAFTPSYDVSPEPLYAAGS
                     RWARALELEVLAPANLVLVGGRAAPPDRPARRVLDALGATTFSLEDVLRDGMATVAQE
                     ALEAAASGTEAVYLSVDMDVLAGVGDPVGLQARELAAGVARVSGALLAAADICGAASG
                     WGGGDGPSAAARVAAEIVAGVAGRLR"
     gene            4076..5173
                     /locus_tag="VFZ86_04130"
     CDS             4076..5173
                     /locus_tag="VFZ86_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005458091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidinotransferase"
                     /protein_id="HEX5641512.1"
                     /translation="MDPHTAPKTPIVNAFNEWDPLEEIIVGRVDENTLVPPWDVIMPA
                     VVHDRTQWQFFKQHGGTPWPVEMLKKAERDLAAFIDVLEQAGVTVRQPEPYAYERPLA
                     TPDWGVASSCYALMPRDVLLVIGDQIIEAPMGWRSRSTERLAYRELCKEYFRGGARWV
                     AAPPPRLSDDSFTPGFVAPEEDEPQRFLPTEYEPMFDAADAIKCGRDVFIARSSCCNR
                     FGIQWLQRHLGDEYTVHEIEVHDTHPMHIDATFQPLAPGKLLINPERVVKVPDMFEKA
                     GWDVLVCPEPEMPESHPMYNCSRWIMMNVVMLDETRVIVSAGEKRFARQLRDWGFEPI
                     ECPFWDFETIGGGFHCASVDIRRRGSLTSYF"
     gene            complement(5157..11360)
                     /locus_tag="VFZ86_04135"
     CDS             complement(5157..11360)
                     /locus_tag="VFZ86_04135"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX5641513.1"
                     /translation="MRVGRADGERWLRLGEAAAELGVSLNTLRRWSDAGTLTCYRSPG
                     GHRRYRGDDVQALLLSSESGSGRTGRLPVQQAPAGDRDERRASLLTLARVAAEGVGVS
                     ECRLSLVDAHGACGLLIAHSRTLESTGVPRDEDGAGEMLPTVREVLRTGRRLVISDLG
                     STSLLERSEAGALRQRGDAAVLAVPVTVESRTRAVLELVESRAPRAFNGANVAFAEFM
                     ARQAANLLVEGGGDREPAAGLPDESVPRATPPSPRPEDLLLTLADRLRHELDAVACDV
                     LRLHDEAQALEPVAASATGDAPPLRGVLYTVADFDEAASALVSGDPVGIDDLAEIDAA
                     GPHLVRRERSGARTVNATPIRLGHEVVGLLELYSREPGRRLGREEQALVDAAAATAAL
                     ALTDQHDTAVLARRIAQLDDMIAGFGTHSPAMDAESLVQSTLDAIRRRPDFDACTVYR
                     VEDAVATPFPAGDDAGNPVGDGGSWRLGDYPAAAQAVAGRAPVIVLADSDQPLMSPAA
                     TARFVTERGLAGVVLTPVVFWDRVVGVLEFGSAAAAGVATAAQVAQVAADLLAAALGS
                     GEVISRLQRRNRDLGLVVEAGLEDTARLSTDEILHAVAERLSELTRTPVTDIYAVEGD
                     TLRGLVSYDGGRFDAEWDGVVLPLRRYPCSRKAVETGEVTVVAALDDPLLDEEGRYSL
                     ERWGYQSQLSMPLVSQGRVLGVAELSDYEPRDFAEDMDLIRGLGQVAAHALDNASLFE
                     QVDRRNRTLNELVELSSLASHSRDLDQLVRSVAERVLSAVDAANCDIYRSMEGVLLCV
                     ASFDRSGHDDSVLGGKFDLERYPTTAEAVYGHQILTITSPDDPQLSQAERDTYHDYGF
                     SSEVCLPLVIGDELYGLLDVYDTRERDFAEYLGFLRGTAQALAGAFENALLVDQLERR
                     TTILRDVVELGAVASQAHDLETVLTALAVRLRETVGAADCDIFTLKDGMLRCMVSADK
                     DGLDASVVGRILDVDRFPATALAVRTGQPMAIASLDDPHLTDEEREDMAEYGFESELC
                     IPLLGGDRVIGLIDVFDTRPRDYAEYLDFLRSVGQTAAGAIENAVLLEKLERRNAALA
                     ELVELGRTASDAGGLTALVRSVGPRVVELMEADGCQVFILRGDSLHCVLTYDDGQFLD
                     DYADRTLDLDLFPSTRAAIAEKSALIIETPEDPRLSDYERGLYLESGSQSEICVPLVL
                     EDRVVGLLDVYDHRRRDYAEHRDFLLRVGQMMAGALENALLMERLEETNETLGLLVES
                     GIEFGATLDRDRLLESVALRLCAAAAAPCCEITTLHGDALRVVARMDHGEADGDHIGT
                     EYPLDHLGLMRIALETRRPAYAEDITDDPRVSDLEREGDPRRGHRAMLCLPLTSRGEV
                     IGVAVVHDDRARTFTRTDLLHSLAQVAAGALANAELYEGIKSMHLGNLKALSSALNAK
                     DYYTLGHAARVAAYMALLGRELGWPDEFLRDVEEAAYLHDIGKIGVPDRVLMKPSGLN
                     EHEWRLMRQHPMFSAEIIHSLFAESLVAGVRHHHERWDGGGYPDGLAAEEIPSVARAM
                     CVADSYDAMSFRRPYRQGLTDEECLEELERCSGVQFDPAMVAAFLRVLARVAEGRRDA
                     AAVAGRAAAALTVEECLALREARGEIAPGRAAVAEKLRQTRAEDARTKHLTLYGRYGR
                     RTVVLADSEAGAGPDSPQPGDEVVSDDELAEAFAGRDLPANVLYVDQWGVWISGVAPV
                     TDADGSVVAVVSADVPAAEGVTEVEGLRSNVAETFASMLHNAAAQSGRTELEAITDGL
                     TGLYNHRYFHERLSEEIERCQEQGTSLALLFCDLDDFRAFNDLHGHGSGDKALRAVAR
                     VLESSVRHVDLVARYGGEEFAAILIDTTEPGALEVAERMRAGITHIRLGAGADTLSVS
                     IGVATCPRDATFKEELVDKADWAMYLAKRRGRDTVMTFGAQHGGDTPEQAAVVRPDHV
                     AAMGELVAAREAFRQRRRAAVTQIALGVALATGVPAEDVHAAIGAGTPSATPAGRIVA
                     LTETYQALVTERPYRARISEAEALDELLKCPALDGEDELARAFSEVLRSRT"
     gene            complement(11605..12708)
                     /locus_tag="VFZ86_04140"
     CDS             complement(11605..12708)
                     /locus_tag="VFZ86_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016907577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HEX5641514.1"
                     /translation="MTQEDVLRALSSVKDPELQRDIVDLGMVRDVVADDARLGATIVL
                     TTAGCPLRNQIRADAEQALGAIAGGRSVEIKLDAMSCDERDALAGRLRQRDQLVQSLF
                     GPGSRTRTIAVASGKGGVGKSTVTVNLATALASRGRAVALIDADVYGPTVPLMMGLPA
                     VPPETHDGKMLPPEAHGVKVVSMGFFLPDNEPVVWRGPMLGRAIEQFLGDVLWGSPDY
                     LLIDLPPGTGDIALTIAQMIPGAELVVVTTPQEAAARTAIKAAKMAQMTKQRILGVVE
                     NMAYFVCPDCGGRHYIFGRGGAFEIARLMDTRLLGRIPLDESTREGGDIGVPAAVDPT
                     SPVGAAFAEIAKAIDDGAEAPGSESEAGEPAHT"
     gene            13008..13454
                     /locus_tag="VFZ86_04145"
     CDS             13008..13454
                     /locus_tag="VFZ86_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641515.1"
                     /translation="MVAVVAAVALSVVAVAYGATRTQTERRAAGNGACGVLMNDPEAL
                     DAMQALRTEHLKERQAWYKQFGSDPASAEAQTALQDLREEHWNDMRGLLEKLGIELPE
                     GAGPGGMMGRGGGCGGGCGAGGAGAGQGAGYGGMMGSGGGMMGNWN"
     gene            complement(13720..14793)
                     /locus_tag="VFZ86_04150"
     CDS             complement(13720..14793)
                     /locus_tag="VFZ86_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-erythro-3,5-diaminohexanoate dehydrogenase"
                     /protein_id="HEX5641516.1"
                     /translation="MKEGNKFGAHRVIEPQGVLPQPAWKVDNTMEIYDNEILIDVDTL
                     NIDAASFTQIEEAEGGDVHKMARHSLDIIASRGKHHNPDTGSGGMLLGTVAAIGPKLK
                     DRDLKVGDRIATLVSLSLTPLKVDEVISVNRDTCQVKVKAQAILFETGIYAKMPADMD
                     EPLALAILDVAGAPAQAAKLSKPGQTVFIIGAGGKSGLLCAYEAKKRVKPTGKVICLV
                     HGDAGKKRLEKSGFADVIIQGSATDQLFVYEEVKKATDGLMADLTINCVDIPNTEMAS
                     VLATKDDGTVYFFSMATSFTSAALGAEGIGADTLMIIGNGYTKDHAVISLEVVRESPV
                     LMEIFKGTYATGAGNPEPVGLKA"
     gene            complement(14888..16081)
                     /locus_tag="VFZ86_04155"
     CDS             complement(14888..16081)
                     /locus_tag="VFZ86_04155"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009332842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEX5641517.1"
                     /translation="MSDNDIVIISAVRTAQGRFQGGFAGTPAQELGAVAMTEALKRAG
                     VTGDQLDEVIFGNVIQAGLGQNVARQAELKAGIPDSVPAITLNRVCVSSASALAWAAA
                     NIKAGDGELYLVGGTENMTRAPWLLQDGRSGYRMGMPSQEMFDAMVLDGLWCAIGGTH
                     MGLTAENLSEQFGISKELQNEVAHQSQLRATAAIKDGRFKDEIVPVVIPQRKGDPKVV
                     DTDECPRETSPEILAGLKPVFKKDGVVTAGNASAIADGASALVVTSRKKAAELGLKPI
                     AQLVSYATAAVDPSIMGLGETKAAQMALEKAGMTSKDVQLAELNEAFACVAALAIREL
                     GLSSDIVNVNGGAVALGHPLGNTGTRMIVTLIHELQKRGQEIGLTGACVGGGQGAGSV
                     IKVEG"
     gene            complement(16188..16922)
                     /locus_tag="VFZ86_04160"
     CDS             complement(16188..16922)
                     /locus_tag="VFZ86_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OAM dimerization domain-containing protein"
                     /protein_id="HEX5641518.1"
                     /translation="MLIRPYGDTRDDGVVQFSFTLPMPYSVKAADVGKQFLEKLGFHT
                     VGIAECKAIDPDYTFFVAYGRTDTGIDPDEVKGDYLEVEEMDYYAINALIKERLGRKL
                     GVVGAAIESDAHTVGIDAIMNMKGFAGNYGLERYPEMDALNMGSQIPCAQLLRTALER
                     GADAILVSQIVTQKNIHVQHLTQLVELLEAEGVRERFVLIVGGPRINNAFAKELGYDA
                     GFGPRTTPLEVASFLAQEVVRRHESA"
     gene            complement(16922..18532)
                     /locus_tag="VFZ86_04165"
     CDS             complement(16922..18532)
                     /locus_tag="VFZ86_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013976612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 5,6-aminomutase subunit alpha"
                     /protein_id="HEX5641519.1"
                     /translation="MNDALRLNGDLVDRARTAANAIADDIQQHIEEHTTDTTERATLR
                     LLGIAGVNEIDVPLVNVVAEHARELLPGGIMRPFVDLMNQTGKAAQQVAEAVAAGDLA
                     LAEVPAERRAAAEARAEELAREGVARIDAVRARRNELVAQTGEPLKPYVYVIVATGNI
                     YEDAAQAKAAARAGADVIAVIRTTAQSLLDYVPFGETTEGFGGTYATQENFRLMRAAL
                     DEVGEELGRYIRLCNYASGLCMPEIATMGALERLDMMLNDSMYGIIFRNINMQRTFVD
                     QYLSRLINARAGIIINTGEDNYLTTADAFEKGYTVVSSDFINEAFALRANLEPWQIGL
                     GHAFEINPQTPDQVVYQIADAQLVRQLFPGYPLKYMPPTKYMPGDIFHGHIIDGMFNF
                     TGIFTGQEIMLLGMLTEALHTPLLQDRYLSIKNAKYVFESCRDLAGEVTYKPGGMIEQ
                     RANDQLQKATEQLEHVRDISLFRALEDGEFADVKRDPKGGRGFEGVFKVTPDYFNPFF
                     AALRDGRMDGAPEGPPPGGTAAKTEGGH"
     gene            complement(18550..19686)
                     /locus_tag="VFZ86_04170"
     CDS             complement(18550..19686)
                     /locus_tag="VFZ86_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641520.1"
                     /translation="MTHNGGTSATGLRPDGSAVGALYEIIAGSGSRSLAVVGLVKNAG
                     KTTVVNALMANSPRRFGLTSLGLDGERTDHLTGLAKPSITPPAGTLVATTQGSLDRSR
                     YAMETLEVLPFHTPLGRVVIGRAAGDGAVEVSGPTTLAELRVTVERLQALGADQVIID
                     GAINRIGSASPRVSDGVVVATGGMVGDTLDDVLATTVGALDLLMLPGVAPQRRSLMER
                     HVAAGSRVVAFTPDGLAHTLELASVVGEGVTVAREVERLGADTLLVGGALTQEFADDF
                     TRVLSPRAEVSVVVRDATVLVLPPAMVYRFLRRGIRLETLTPLRVLALTVNPFRVPQP
                     YRPKAFFSSVAEAVGGRVPMFDVVNGLSRLPEGGCASPESSPSS"
     gene            complement(19683..20414)
                     /locus_tag="VFZ86_04175"
     CDS             complement(19683..20414)
                     /locus_tag="VFZ86_04175"
                     /inference="COORDINATES: protein motif:HMM:NF019947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX5641521.1"
                     /translation="MLTHPLDERLQALLPIVRGLAQMFGPDCEVVLHDVSRLPHSIVA
                     IENGGVSGRTVGDVPTDRMLRNLRSPDQAQDVRLYVSSHEGKILKSLAVTLRGADGEP
                     YGLLGLNYDVSEVVQAQRVLADFTAIGRLGGGGPELGEIFAGDIRDVVAGMVTQTLGE
                     IGKTPAAMAREEKMEVVKRLEERGAFLVKRSAEQVAEALDLSRYTIFAYLKEIRHGEE
                     GRGAAAQAGRDAGVAVPGRAAGGRR"
     gene            complement(20647..21837)
                     /locus_tag="VFZ86_04180"
     CDS             complement(20647..21837)
                     /locus_tag="VFZ86_04180"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX5641522.1"
                     /translation="MKLKGFLAGLAGGAVAAAAVVLLLVFVFDIGAVSKTVVQATPET
                     PTVYSTPSASTTGGGLTPEQIYSTLSGGVVMVLAEFGGSGTDQFGQPQSAQALGTGFV
                     VDSEGYILTNAHVVDENGQRANSVTVVFNKGGSETQRVPGQLVGVDIGSDVAVIKVDP
                     AKVDLKPLPLGDSDDVVVGEPVVAIGNPLGYDFSITSGIVSATGRSLQAPNGQTIPNG
                     IQTDAAINQGNSGGPLIDSAGNVIGINEQIATSGGGNQGLGFAVPINTAVRSLEQIKS
                     DGKVTYAWMGVSLQTLTSDIAGMFNMQTQGGALVQAVSPDSPASKAGIQGGDQTVTVQ
                     GEQFTIGGDVIVKVDNTEVKTADDLIAYLGTKKPGDTVTVTVERDGKTEEVDVTLAER
                     PSNL"
     gene            complement(21966..23789)
                     /locus_tag="VFZ86_04185"
     CDS             complement(21966..23789)
                     /locus_tag="VFZ86_04185"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5641523.1"
                     /translation="MFSSVKNWLTLLIVALVALAMLVAWVYVVPPLQGRLTDRKLIDA
                     RENARLVGQTLTAPGLMSYDTNRGQLVIVDSDAVTQTLNIIGARFSGRAIIYARSLVP
                     VRDSSSQTAPDVSDYPMLEETVRTGRVTQGTVTTATGRAAATAVPLFTKLTGTTAVGA
                     VLVVQSLEDVDNAVTAVQRQLLFATALALGISLLLGYMASYFIARRLKRIERSAEAIA
                     GGDLSAKVPETVEDEIGQLADTFNVMADRLRSAFAQVEYERDRVEVLLNDLSEGVIGV
                     SAEGKVTIANPAAAILLDAPALVGETVDDALPADVAQLWRDSQRDADFDMVVFAHDDV
                     TLEATTYPVGGDADFTSIVVLRDVTAQARLERARRDLIANASHEFKTPLFSLAGSLEL
                     IDEGELSPEEQREFLQIMRQQVDRLRTMAVSMLDLSRVEAGSFELNPEDVDLVVVGRS
                     VLDEFQAQAQTKAVALSFRGEEGETAWCDEQRLVQVLRALIDNAIKYSPSGSSVRLSS
                     RLEEKEAVVVVADDGPGITRSELPHVFERFHRGRDERATTAGAGLGLSIARELTEMMG
                     GSITAESPPDGGARFTVRLPRAAGGRRRRASSERSGGVHTP"
     gene            complement(23831..24538)
                     /locus_tag="VFZ86_04190"
     CDS             complement(23831..24538)
                     /locus_tag="VFZ86_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011943665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5641524.1"
                     /translation="MAESGARILLVDDEEAILKLLRFPLEKEGYQVVTARDGDEALET
                     FTREDFDLVILDIMLPHVDGMEVCRRIRAQSIVPIIMLTAKSDEFDKVLGLEIGADDY
                     ITKDKFSLREFRSRVRAQLRRAEMSRSAEATKKEVIKVDDLEVDVLKRNVIVRGAKVD
                     LTYIEFEILKTLISHPGRVYSRQLLLQLVWGDSDYRDSRTVDVHIRHLREKLEADPKD
                     PEFIFTVRNIGYKFREF"
     gene            complement(24538..25194)
                     /locus_tag="VFZ86_04195"
     CDS             complement(24538..25194)
                     /locus_tag="VFZ86_04195"
                     /inference="COORDINATES: protein motif:HMM:TIGR04038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family nuclease-associated radical SAM
                     protein"
                     /protein_id="HEX5641525.1"
                     /translation="MTATPAFAGTIAYRAGRSIYLNLTNRCSCSCAFCLREWTDGVFG
                     EDLRLDREPELEEVTQAIEVAFLDGPADEVVFCGFGEPTMRLDVVLAVTEWLRLRRIR
                     SRLDTNGHGALLNPDLDVPAALAKAGLDAVTVSLNAATPEEYDRACRPVFTKSYRAVL
                     RFAEQCLAHGMETTLTAVDYPGADLPGVADIAAAMGAGFRARGYAAPGARPAAEKGDH
                     "
     gene            25296..26243
                     /locus_tag="VFZ86_04200"
     CDS             25296..26243
                     /locus_tag="VFZ86_04200"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX5641526.1"
                     /translation="MTDVTDSLITLCGRLREVVLPSLGTHAARGHAGVAVGGDVTFSI
                     DEQAEALLVEHMREHLPAWAFYSEDGGLQGASDPELILIVDPIDGTRPAAAGFEMACV
                     SVAAVPAGPAPTMGGVVAGVIQEIKNGDLFVAEKGAGFHMRRADGAAIPFLPTPRSDL
                     ESIFWTLGFRGRPAVILASVLEELIDVSSVGGCVFDIGSATYGITRILTGQLDAYVDI
                     GPAIIAAHPWTEAEFRRVGRGAVLCNSPYDVAAAYLLCKEAGLPFGDADGSSLDDRPV
                     LGSDASFQMATVAAGNQELQDALVESVQRGIAGLRRPEA"
     gene            26247..>26844
                     /locus_tag="VFZ86_04205"
     CDS             26247..>26844
                     /locus_tag="VFZ86_04205"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX5641527.1"
                     /translation="MAENLTDWHEAMLYEQLGEGVVQCHVCPRLCRVAPGKSGVCRAR
                     VNRDGVLYAITYGKVCSVAADPIEKKPLYHFFPGTTVLSLGSLGCNFACRHCQNWQIA
                     HADPGEATRDLRDLPIRNLPVLAANNECQGVAWTYNEPTIWLEYILDGAQAAHEHGLY
                     TVMVTNGYITDDALSILGPHIDAYRVDVKSFTNQQYKEL"
BASE COUNT         3951 a         9070 c         9567 g         4256 t
ORIGIN      
        1 aaggcggcgc gctgctcgtc gtagtcggcg acgacgatct cggtgacggc cgggttgcgg
       61 ccgaggttcc aggtgaggac ctgaccctgc atgccggcgc cgacgataag gaccttcatc
      121 gtgtctctcc ctccgtgtga ctacgcccgg acgggcgcga ggactgcctc tcacatcgca
      181 ggttgtacca ccgcacgccg ggagaggcaa gcgtttgagg gaacgattat cgtacatcga
      241 gagatagatg gcagcgaaag tcgttcctca gacgacccag gcaccccgta ttcgcgttcg
      301 tctgatcgag gcagctgacg cgccggcgag cggcgaccgc cgccgtgctt ggcggcaggc
      361 gtggtagaac tccgaggagg actggccaca gagcggagga gcaccgtgca cgagctcggc
      421 atcacacagg ggatcatcga cagggcacgc gacgcggcgg tggccaacgg cgcccgccgg
      481 gtcgccgggc tccacttgct gatgaccccc gccgcggact tcacgcagga ctccatagag
      541 atgtacttcg agatgctcgc gagcgacgac gagctcttca ggggggcgaa gctccacttc
      601 gagcacaggc cggtcgccgc gacctgcctc agctgcagtg acgagttcac caccgacgct
      661 ccgcacccga tctgcccgca ctgcggctca cagctggtgc gactggatcc cgacgcgccg
      721 atggtgcagc tcaccgacat cgacatcgac gaggggtcgg acgagtcgtc gtgaaggcgg
      781 gagcggtggt ggagcatagc ggaatcgaac cgctgacctc ctgggtgcga tccaggcgct
      841 ctcccagctg agctaatgcc ccacgcggtc ccggcgtgcc gggacaacgt agtatgcggc
      901 attcccgggc aaccggcaag tcagtcaggc tgccgcgggg ccggcggtgg gggcgcctcc
      961 ggccaggagg ccgcttgcgg cgcgggcggt ggcgcggccg gccacgcggc cgcggccggc
     1021 gggaccgccg tgagcgaggg gtcgaaggtg aacggcgggt agcggtccac cagtccgagg
     1081 gcgtaggcgg tcacacgcgt gtaccagcga acgtatccgg ccaccaggtc ccacatgccg
     1141 cgcgggaacg tcgccccgaa gaggatcacc cactgcacca cgaaccagag cacgaacgcc
     1201 gcgatgccga cgaagatgag cacgacgtag tgcgggaagg cggccagctg ccgcagcagc
     1261 agcccgccgc cgccccacga cggcaggttg agctggtagt cgtaactgcc gtccgtcgac
     1321 caggacgcga tcgtgtccgc gtggtcgatc agccaggcgg ccatccagat gagggcgatg
     1381 aggccgagga tctggacgac gatcgtgaag acggcataca cgcggctcgg ctgggccggg
     1441 cgctccgcca ccacatcgac ggggtagtcg tccaccggct tgagggcgaa gggcgggtac
     1501 cggtcgtcga cggagaggac gaacgcggtc acccgcgtct gccacctcag cacgccggtg
     1561 acgaactcgt gcatgccggc cgggtactct cccttgaacg ccacgacgaa ctgtgcgacg
     1621 aaggcgacca cccactgggc gatgccgagg aagatcagga cgatgccgtg cgggatcagc
     1681 gcgaagaact tgatccagaa gatggtgagc gccgcccagc cccgcgagct cctctcgggg
     1741 tagtcggcgc ggacgtccac cggatagtca gtcggagcca tgatccccct ctcgtgccgg
     1801 cctcacgacc ggatcagaac agcggccgca gccgcgccgc catctcctcg ggcgcggcga
     1861 acatcagctc accgcccagc tgcttctgca ggaagtagtc gtcggtgcgg taatcctgtt
     1921 tgaacgcggt acgggcgatc acgcgcacga cgacggggaa ccccgtgtct cgcccgtcca
     1981 gcggcccccc cgagagcgtg aagttgaacg ccgagagccc ctcgtcctca taccaggtga
     2041 ggaccttcgt gaggccgtgg gcgagcgccg cggcgtgttc gtcccggagg tccgtcaggc
     2101 gcggcgcgtc gggcacgacg gcgatcgcct cgcgggcgcc ggccggcgcg aagggcacca
     2161 gccagtggac accggcggct ccccagacgt agcgctcacc cgcggcgcgt tcctgctcga
     2221 ccagcgttct ccagtagctc tccccacgcg cgccgagcca ggcggccgag cggtcccagt
     2281 acagctggac cagccagggc acggtctccc cgccgaacac ctggaagtgg gggtgcacca
     2341 ggctggcgcc gccgggcagc atgtgattgc agcagacgga gatatgggtc atgcgggggt
     2401 ggaacgcagc ggcgcggcgg gcgaagtcga gcgccgcgca cagaccctcc tcgagcaggc
     2461 tcggcgtgaa ctgcgacggc cgcaggaagt gctcccccgg gaacgtcacc acagcgtgca
     2521 cggccgcgag ggggaagagg ttggggaaga cgagagccct cccgcgctcg aggcgcccgc
     2581 cgggcagcat ctcgtcagga tagcgaggcg tggcctccag cacgttctcc gggcagaaga
     2641 agcacccctc gcggctgcgg gcgaccagct ggtcggtgta cgcccagtcg gccctgccga
     2701 ggaacatctc ctccttggcc tcgaggccgg cggaagcgac cgcggtcatc ccggtcaggg
     2761 ggtcggtgcg cacctcgacc cgatcttcgg cgtactccat cccggcgaga gggctgtaga
     2821 acgcggcggt ctccgtgcgg cgatggaact cgatgggctc ggtcatgacc gctcctctcg
     2881 tggtgcggcg gtccccctcg gggcgggcgg cacgatgata cggccggtcg tggagcccga
     2941 caaggccgac gcggccgccc atggcataga ctgggtgcgc cccagtcccc gacctcgcgg
     3001 agcccgcatg aggaccatcg ccgtgcgcaa ccatgccccc gggccgaccg tgggcaggaa
     3061 gacggcgcac gagggcggcc ggtgaggtgc tgatggaggc cgaggcgctc aggaagctct
     3121 ggggcgacgc gggcggcggc ggggacccgg cgcccaaggg cgccggcctg ctcggtttgc
     3181 acgtcgtcga cgacccggcg gcgctgaccg ggatggcgct ggcggtgctc gggcttccgc
     3241 tggatgacgt cgccggacgc ccggcggccg tgaggcgcga atcgctgcgc tatgcggatt
     3301 ggctgccggg cctgtccacc ggcttccggg cggccgacta cggcgacgtc gcggtcgccg
     3361 aggaagaccc ggccctcgcc ttcatgctcg cgcacagccg gctggccgac atcgtcgccg
     3421 ccggcgcgtc gccgctggtg ctgggcggcg acgccctcgt gagcgttccc gtgctgcagg
     3481 tgctctccgg caagctgcgc ggccggctgg ggatagtcgc tttcacacca tcctacgacg
     3541 tctcgcccga gccgctgtac gctgccggct cgcgctgggc gcgagctctc gaactcgagg
     3601 tgctcgcgcc cgccaacctg gtgctggtcg gcgggcgggc ggcaccgccg gacaggcccg
     3661 cccgccgcgt gctcgacgca ctcggtgcga cgaccttctc tctcgaggac gtccttcgtg
     3721 acggcatggc caccgtcgcg caggaggcgc tcgaggcggc cgccagcggc acggaagcgg
     3781 tctacctctc cgtcgacatg gacgtgctgg caggcgtcgg cgatcccgtc ggtctgcagg
     3841 cgcgtgagct ggcggcggga gtcgcccgcg tctccggcgc cctgctcgcg gcggcggaca
     3901 tctgcggcgc cgcttcggga tggggcggcg gcgacggccc gtctgccgcc gcgcgcgtcg
     3961 ccgccgagat cgtcgccggg gtggccggca ggctcaggta gaaccaacgt accgccggcg
     4021 ggcccgtgcg atcggctcgc ggggcggctc gaccgacagt ccgcaaggag gaccagtgga
     4081 cccgcacacc gcgccgaaga cccccatcgt caacgcgttc aacgaatggg acccacttga
     4141 ggagatcatc gtcgggcgcg tcgacgagaa cacgctcgtg ccgccgtggg acgtcatcat
     4201 gccggcggtg gtgcacgaca ggacgcagtg gcagttcttc aagcagcatg gcggcacgcc
     4261 gtggccggtg gagatgctga agaaggcgga gcgtgacctc gccgccttca tcgacgtgct
     4321 cgagcaggct ggggtcaccg tgcggcagcc cgagccgtac gcctacgagc gccctctggc
     4381 gacgccggac tggggggtcg ccagcagctg ctacgcgctg atgcctcgcg acgtcctgct
     4441 ggtcatcggc gatcagatca tcgaggcccc gatgggctgg cggagcaggt ccaccgagcg
     4501 gctcgcgtac cgcgagctct gcaaggagta cttccggggc ggcgcccgct gggtcgccgc
     4561 gccgccgccg cgtctctccg acgacagttt cacgcccggc ttcgtcgccc cggaagagga
     4621 cgagcctcag cgctttctgc cgacggagta cgagccgatg ttcgacgcgg ccgatgccat
     4681 caagtgcggc cgcgacgtct tcatcgcccg ctcgagctgc tgcaaccgct tcggcatcca
     4741 gtggctgcag cggcacctgg gcgacgagta caccgtgcac gagatcgagg tccacgacac
     4801 gcacccgatg cacatcgatg cgaccttcca gccgctggct ccgggcaagc tgctcatcaa
     4861 ccccgagcgc gtggtcaagg tgccggacat gttcgagaag gccggctggg acgtcctcgt
     4921 ctgccccgag ccggagatgc ccgagagcca tcccatgtac aactgcagcc gctggatcat
     4981 gatgaacgtg gtcatgctcg acgagacccg cgtgatcgtg tcggccggcg agaagcggtt
     5041 cgcccggcag ctcagggact ggggcttcga gccgatagag tgccccttct gggacttcga
     5101 gacgatcggc ggggggttcc actgcgccag cgtcgacatc cgccgccgcg gcagcctcac
     5161 gtcctacttc tgagcacctc ggagaacgcc cgcgccagct cgtcctcccc gtcgagcgcc
     5221 ggacacttca gcagctcatc gagagcctcc gcctccgaga tgcgggcgcg gtacggacgc
     5281 tcggtcacga gcgcctgata ggtctccgtc agcgcgacga tcctgccggc cggcgtagcg
     5341 ctgggcgtcc cggcgccgat ggccgcgtgg acgtcctccg ccggcacgcc ggtcgcgagc
     5401 gccacaccca aggcgatctg cgtgaccgcg gcgcgccgcc gctgacgaaa ggcctcccgc
     5461 gcggccacca gctcgcccat cgcggcgacg tggtcgggac ggaccacggc ggcctgctcg
     5521 ggcgtgtcgc cgccgtgctg cgcgccgaac gtcatcaccg tgtcgcgccc gcgccgtttg
     5581 gcgaggtaca tcgcccagtc cgccttgtcg accagctctt ccttgaaggt cgcgtccctg
     5641 ggacacgtgg cgacgccgat gctcaccgac agggtatcgg cgccggcgcc gagccggatg
     5701 tgggtgatgc ctgcgcgcat gcgctccgcg acctcgagcg cgccgggctc ggtcgtgtcg
     5761 atgaggatgg cggcgaactc ctcgccgccg tatctcgcga ccaggtccac gtggcgcacg
     5821 gagctctcga gcacgcgggc gaccgcgcgc agcgccttgt cgcccgaccc gtgtccgtgc
     5881 aggtcgttga aggcgcggaa gtcgtcgagg tcgcagaaga ggagcgcgag actggtcccc
     5941 tgctcctggc agcgttcgat ctcctcactc aggcgctcgt ggaagtagcg gtggttgtag
     6001 aggccggtga gaccatccgt gatcgcctcg agctcggtac gacccgattg cgcggcggcg
     6061 ttgtgcagca tcgaggcgaa ggtctcggcc acgttgctgc gcagcccctc gacttcggtg
     6121 acaccctcgg ccgccgggac gtccgcggag acgacggcga ccacggagcc gtccgcgtcg
     6181 gtcaccggcg cgaccccact gatccacacg ccccactggt cgacgtacag gacgttggcg
     6241 ggcagatcac ggccggcgaa cgcctcggcc agttcatcgt cgctcacgac ctcgtcgccg
     6301 ggctgcgggg agtccgggcc ggcgcctgcc tcggagtcgg caaggacgac cgtccgccgc
     6361 ccgtaccgcc cgtacagcgt gaggtgcttc gtgcgggcgt cctcggcgcg ggtctgccgc
     6421 agcttctccg ccaccgcggc gcgtccgggc gcgatctcgc cacgcgcctc gcgcagcgcg
     6481 aggcattcct cgacggtcag cgccgccgcc gcccggccgg cgacagcggc cgcgtcgcgg
     6541 cggccctccg ccacgcgcgc cagcacccgg aggaacgccg cgaccatcgc cgggtcgaac
     6601 tgcacgccac tgcagcgttc cagctcctcg aggcactcct cgtcggtgag gccctgacgg
     6661 tacgggcgcc ggaacgacat ggcgtcgtac gagtccgcca cgcacatggc gcgggcgact
     6721 gaggggatct cctcggcggc gagtccgtcg gggtagccgc cgccgtccca gcgctcgtga
     6781 tgatgtctga cgccggccac gagcgactcg gcgaacagcg agtggatgat ctcggcgctg
     6841 aacatggggt gctgccgcat cagtcgccac tcgtgctcgt tcagcccgct cggcttcatc
     6901 agcacccggt cgggcacgcc gatcttgccg atgtcgtgca ggtaggccgc ctcctcgaca
     6961 tcgcgcagga actcgtccgg ccagcccagc tcccggccga gcagggccat gtaggcggcc
     7021 acgcgtgcgg cgtgcccgag ggtgtagtag tccttggcgt tcagcgccga gctcagggcc
     7081 ttcaggttgc cgaggtgcat gctcttgatg ccctcgtaga gctccgcgtt ggcgagggcg
     7141 cccgcggcga cctgcgcgag gctgtggagg aggtccgtgc gcgtgaaggt gcgggcgcga
     7201 tcgtcgtgga ccaccgctac gcctatgacc tctcccctgc tggtgagcgg caggcacagc
     7261 atggcccggt gtcctctgcg gggatccccc tcgcgctcga ggtcgctgac ccgggggtcg
     7321 tcggtgatgt cctcggcgta cgccggccgc cgcgtctcca gcgcgatgcg cataaggccc
     7381 aggtggtcga gcgggtactc cgtgccgata tggtcgccgt ccgcctcgcc gtggtccatg
     7441 cgggccacga cacggagggc gtcgccgtgc agcgtggtga tctcgcagca cggagcggcg
     7501 gcggcggcgc acaggcgaag ggcgacgctc tcgaggagtc ggtccctgtc cagcgtcgcc
     7561 ccgaactcga tcccactctc caccagcagc ccgagtgtct cgttggtctc ctccaggcgc
     7621 tccatgagca gggcgttctc cagggcgccg gccatcatct ggcccacgcg caggaggaag
     7681 tcgcggtgct cggcgtagtc gcgccgccgg tggtcgtaga cgtcgagcag cccgactacc
     7741 cgatcctcca gcaccagcgg cacgcagatc tcactctgcg atcccgactc gaggtagagc
     7801 cctcgctcgt agtcgctgag gcgcggatcc tccggggtct cgatgatcag cgccgacttc
     7861 tccgcgatgg ccgctctggt gctgggaaag aggtcgaggt cgagcgtgcg gtcggcgtag
     7921 tcgtcgagga actgcccgtc gtcgtaggtg aggacgcagt ggagggaatc accgcggagg
     7981 atgaacacct ggcagccgtc ggcctccatg agctccacga cacgtgggcc gaccgaacgg
     8041 accagcgccg tcaggccgcc ggcatccgaa gcggtcctgc ccagctcgac gagttccgcg
     8101 agcgccgcgt tgcggcgctc gagcttctcg agcagcacgg cgttctctat ggccccggcg
     8161 gccgtctgac ccacgctgcg caggaagtcg aggtactccg cgtagtcacg cggccgggtg
     8221 tcgaagacgt cgatgaggcc gatcacccgg tcgccgccga ggagcgggat gcagagctcg
     8281 ctctcgaagc cgtactcggc catgtcctcg cgctcctcgt cggtgaggtg cgggtcgtcg
     8341 agactggcga tcgccatggg ctggccggtg cgtaccgcga gcgcggttgc ggggaagcgg
     8401 tcgacgtcga ggatgcggcc cacgacgctc gcgtcgaggc catccttgtc cgcgctgacc
     8461 atgcagcgca gcatgccgtc cttcagcgtg aagatgtcgc agtcggccgc gccgacggtc
     8521 tcgcgaagcc gcacggcgag cgccgtgagc accgtctcca ggtcgtgggc ctgcgaggcg
     8581 accgccccca gttcgacgac gtcgcggagg atcgtcgtcc tccgctccag ctggtcgacg
     8641 aggagggcgt tctcgaacgc cccggcgagc gcctgcgccg taccgcgaag gaagcccaga
     8701 tactcggcga agtcgcgctc gcgggtgtcg tacacgtcga gcagcccgta caactcgtcc
     8761 ccgatgacca gcggcaggca cacctcactg gagaacccgt agtcgtggta cgtatcgcgc
     8821 tcggcctgac tcagctgggg gtcgtccggg ctcgtgatcg tgaggatctg gtggccgtac
     8881 acggcctctg cggtcgtcgg atagcgctcg aggtcgaact ttccgcccag caccgagtcg
     8941 tcgtgcccgc tgcggtcaaa gctcgcgacg cagagcagca ccccctccat cgagcgatag
     9001 atgtcgcagt tcgccgcgtc caccgcggag agcacgcgtt cggctacaga gcgcacgagc
     9061 tggtcgagat cccgcgagtg gctcgcgagc gagctgagct cgaccagctc gttcagggtc
     9121 cggttgcgcc ggtctacctg ctcgaagagc gaggcgttgt ccagggcgtg ggcggctacc
     9181 tggccgagtc cacggatgag atccatatct tcggcgaagt cgcgcggctc gtagtccgac
     9241 agttccgcga caccgagcac gcgtccctgg gagacaagcg gcatggacag ctgcgactgg
     9301 tacccccatc tctcgaggga gtagcggccc tcctcgtcca gaagggggtc gtccagcgcc
     9361 gcgaccacgg tgacctcgcc ggtctcgacc gccttgcgac tgcagggata gcggcgcagg
     9421 gggagcacga cgccatccca ctccgcgtcg aaccggccgc cgtcgtagct gacgagccct
     9481 cgcagcgtgt ctccctcgac ggcgtagatg tcggtcacgg gggtgcgcgt gagctcggag
     9541 agccgttcgg ccacggcgtg gaggatctcg tccgtgctga ggcgggcggt gtcttcaagg
     9601 cccgcctcga cgaccaggcc gagatcgcgg ttgcggcgct gaaggcggct gatgacctcg
     9661 ccgctgccga gcgccgccgc caggaggtcg gcggccactt gggcgacctg cgccgccgtg
     9721 gccacgccgg cggcagccgc cgagccgaac tcgagcaccc cgaccacgcg gtcccagaag
     9781 accaccggcg tcagcaccac gccggcgagg cctcgctcgg tgacgaagcg cgccgtcgcc
     9841 gcgggtgaca tcagcggctg gtcggagtcc gccaggacga tgacgggcgc tcgtccggca
     9901 acggcttgtg cggcggccgg ataatccccg aggcgccacg atccgccgtc gccgacggga
     9961 ttgcccgcgt cgtcaccggc cgggaaggga gtcgcgaccg cgtcctcgac gcggtagacg
    10021 gtgcaggcat cgaaatccgg ccgtctgcgg atcgcgtcga gcgtggactg tacgagcgac
    10081 tccgcgtcca tggccgggga gtgcgtgccg aacccggcga tcatgtcgtc gagttgcgcg
    10141 atgcgtcggg cgaggacggc ggtgtcgtgt tggtccgtca gcgcgagcgc cgccgtggcc
    10201 gcagcagcgt cgacgagtgc ctgctcctct cgccccagcc ttctccccgg ctcccggctg
    10261 tacagctcga gaagaccgac gacttcgtgg cccaaacgga tcggggtcgc gttcacggtc
    10321 ctcgctccgc tccgctcgcg gcgcacgagg tgcgggccgg cggcgtcgat ctccgccagg
    10381 tcgtcgatgc ccacggggtc gccgctcacc agcgcggacg ccgcctcgtc gaagtcggcc
    10441 acggtgtaca ggacgccgcg aagcggcggt gcgtcgccgg tggcggaagc ggcgaccggc
    10501 tccagcgcct gcgcttcgtc gtggagtcgc aggacgtcgc aggcgacggc atccagctcg
    10561 tggcggagcc ggtcggcgag cgtgagcagc aggtcctcgg gccgcggtga gggtggagtc
    10621 gcccgcggga cgctctcgtc gggaagcccg gcggcgggct cacggtctcc gccgccctcc
    10681 accaggaggt tcgccgcctg gcgcgccatg aactccgcga aggcgacgtt ggcgccgttg
    10741 aaggcgcgcg gcgcgcggga ctcgacgagt tccagtaccg cgcgggtgcg gctttccacg
    10801 gtcaccggca cggcaaggac ggcggcgtca ccccgctgac gcagcgcgcc ggcctccgag
    10861 cgctcgagca ggctcgtcga gcccaggtcg gagatgacga gccggcgtcc cgtgcgcagc
    10921 acttcccgga cggtggggag catctccccc gccccgtcct catccctggg tacgccggta
    10981 ctctccagag tccggctgtg agcgatgagg agaccgcagg cgccgtgtgc gtcgacgagt
    11041 gagaggcggc actccgacac gccgacgccc tccgccgcga cgcgggcgag cgtcagcagg
    11101 gaggcgcggc gctcgtcgcg gtcgccggcg ggcgcctgct ggaccgggag tctaccagtg
    11161 cggccactcc cgctctcgga gctcaggagg agcgcctgca cgtcgtctcc gcggtagcgg
    11221 cggtgaccgc cggggctgcg gtagcaggtc agcgtaccgg cgtcgctcca gcggcggagc
    11281 gtgttgagac tcacgccgag ctccgccgcg gcttcgccca ggcggagcca gcgttcgcca
    11341 tctgctcgtc ctacgcgcat agagggagaa gggatcggtt gaccggacgt tcagaacttc
    11401 ctcaattctc ccggcattcc tgcccggcgc aagccctcat caagtatggg caggttctct
    11461 gcaggaaggc aggcgaatat gcctgcctga agacagggcg ggccgcgcac ggtgcggctc
    11521 caggcagtcc tcgcgagcgg accctcgcgg cgtccgattc ccgccgggtc cgccggcgcg
    11581 cgtggcgaac gcagtccgtg cgagctatgt gtgtgccggc tccccggcct cagactcaga
    11641 gccgggtgct tccgcaccgt cgtcgatcgc cttcgcgatc tcggcgaacg ccgctcccac
    11701 gggagaagtc ggatcgacgg ccgccgggac gccgatgtcg ccgccctcgc gggtcgactc
    11761 gtcgagcggg atcctgccga gcagccgtgt gtccatgaga cgcgcgatct cgaacgcgcc
    11821 gccgcgcccg aagatgtagt ggcgaccgcc gcagtctgga cacacgaagt aggccatgtt
    11881 ctcgacgact ccgaggatac gctgcttggt catctgcgcc atcttggccg ccttgatggc
    11941 ggtgcgtgcg gctgcctcct gaggcgtcgt gaccacgacg agttcggccc cggggatcat
    12001 ctgcgcgatc gtgagggcga tgtctccggt gcccggcgga aggtcgatca ggaggtagtc
    12061 cgggcttccc cagagaacgt cgccgaggaa ctgctcgatg gcgcggccca gcatcggccc
    12121 tcgccacacg accggttcgt tgtccgggag gaagaagccc atggagacga ccttcacccc
    12181 gtgggcctcc ggcgggagca tcttgccgtc gtgcgtctcc ggcgggactg ccggaaggcc
    12241 catcatgagg gggaccgtcg ggccgtacac gtcggcgtcg atgagggcca ctgcgcgacc
    12301 gcgggaggcg agtgcggtcg ccaggttcac cgtcacggtg ctcttgccca caccgccctt
    12361 cccgctcgcc acggcgatcg tccgtgtacg cgaacctggc ccgaagagcg actggacgag
    12421 ctggtcgcgc tgcctgaggc ggcctgcgag cgcgtcgcgc tcgtcgcagg acatggcatc
    12481 cagcttgatc tcgaccgatc gcccgccggc gatcgcgccg agggcctgct cggcgtcggc
    12541 acggatctgg ttgcgaagag ggcagccggc ggtggtcagg acgatcgtgg cgccgaggcg
    12601 cgcgtcgtcc gcaacgacat ctcggaccat gccaaggtcg acgatgtcgc gttgcagctc
    12661 agggtccttg acgctcgaga gagcgcggag cacgtcttcc tgggtcacgt ttgcctccgt
    12721 gatcgaatcg ctcggagctt gaatcgccct cccgtgtgta cccgcgcgca gggtgtcgca
    12781 gtcggccggc gccatcgtag caggtcacgt cgatgcgccg gcgcagatcg tgtggcgttc
    12841 cccggcacgg caggcgtgct tcaaaggttc ttcacaactt tcgtgtagca tgacggtcga
    12901 aaaggtgcga cctgccgctg aggcggcggt cgtgaggcga tgagcggcgg tgcggaagct
    12961 ccgctcggaa ggagcgcgct gtgagacgac actggaagag gatcgccatg gtggcggtcg
    13021 tcgctgcggt cgcgttgtcg gtggtcgcag tggcgtatgg agcgaccagg acacagaccg
    13081 aacgccgtgc tgcaggcaac ggtgcgtgcg gcgtgctcat gaacgaccct gaggcgctcg
    13141 acgcgatgca ggcgctgcga acagagcatc tgaaggagag gcaggcgtgg tacaagcaat
    13201 tcggctcgga cccggcctcc gctgaagctc aaaccgcgct tcaggatctg cgcgaagagc
    13261 actggaacga catgcgcggg ctgctcgaga agctcggcat cgagctgccc gagggcgcag
    13321 ggccgggcgg catgatgggg cgcggcggcg gctgcggagg cggctgtgga gccggcggag
    13381 caggcgccgg ccagggagca gggtacggcg gcatgatggg ttccggtggc ggcatgatgg
    13441 gcaactggaa ctgagcggaa gatcttccgg ctcgcagcga tgcgagtctg aggagtacgg
    13501 gaaacggggg ccggtgtttc acgtgaaaca ccggcccccg ttctgttgcg aaatgatcgt
    13561 tcgcccgcgc ccttgtcccc gggaggacga aggagtcatg acttccggcg cgctcctgtt
    13621 tcacgtgaaa cgtgcagagg gccccgacgc cgccgtcggg gccctcttgg ctcagtcctg
    13681 caaggtcgcg atccgggggc gcacccgcgg accccggcct caggccttca gcccgaccgg
    13741 ctcggggttg ccggcgccgg tcgcgtaggt gcccttgaag atctccatga gcaccgggct
    13801 ctcgcgaacg acctcgagcg agatcacggc gtggtccttg gtgtagccgt tgccgatgat
    13861 catcagggtg tccgcgccga tgccctcggc gccgagcgcg gcggacgtga agctggtcgc
    13921 catgctgaag aagtagaccg ttccgtcgtc cttggtcgcg aggaccgagg ccatctcggt
    13981 gttggggatg tcgacgcagt tgatggtcag gtcggccatc aggccgtcgg tggccttctt
    14041 gacctcttcg tagacgaaaa gctggtcggt cgccgagccc tggatgatga cgtccgcgaa
    14101 gccggacttc tccaggcgct tcttgccggc gtcgccgtgc accaggcaga tcaccttgcc
    14161 cgtgggcttg acgcgcttct tggcctcgta ggcgcacagc aggccgctct tgccgccggc
    14221 gccgatgatg aagacggtct ggccgggttt actcagcttg gcggcctggg cgggagcgcc
    14281 ggcgacgtcg aggatggcca gcgccagcgg ctcgtccatg tcggcgggca tcttggcgta
    14341 gatgccggtc tcgaacagga tcgcctgcgc cttgaccttg acctggcagg tgtccctgtt
    14401 gaccgagatg acttcgtcga ccttcagcgg ggtcagggag agtgagacga gggtggcgat
    14461 gcggtcgccg accttgagat cgcgatcctt gagcttgggg ccgatcgccg cgaccgtccc
    14521 gaggagcatg ccgcccgagc cggtgtcggg gttgtggtgc ttgccgcggc tggcgatgat
    14581 atcgaggctg tgccgggcca tcttgtggac gtcgccgccc tcggcctcct cgatctgtgt
    14641 gaagctggcc gcgtcgatgt tgagcgtgtc gacgtcgatg aggatctcgt tgtcgtagat
    14701 ctccatcgtg ttgtcgacct tccacgcggg ctgtggcagg acgccctgcg gctcgatgac
    14761 ccggtgggcc ccgaacttgt tgccttcctt catcccttct ccctcccccc gaagggcggc
    14821 cgggcgcggg tggcgtgagc ctgtcccgcg cccgacgcat catgcggggt cgtttcgtgg
    14881 ggcgatatca gccctctacc ttgatgaccg agccggcgcc ctggccgccg cctacgcagg
    14941 cgccggtgag gccgatctcc tggccgcgct tctggagctc gtggatgagc gtcacgatca
    15001 tgcgcgtgcc ggtgttgccg agcggatggc cgagagcgac ggcgccgccg ttgacgttga
    15061 cgatgtcgct gctgaggccg agctcacgga tggcgagggc ggcgacgcag gcgaaggcct
    15121 cgttgagctc ggcgagctgc acgtccttgc tggtcatgcc ggccttttcg agcgccattt
    15181 gggccgcctt cgtctcgccg agacccatga tggaggggtc gacggcggcc gtggcgtaac
    15241 tgacgagctg cgcgatcggc ttgaggccga gctctgcggc cttcttgcgc gaggtcacga
    15301 cgagtgcgct ggcgccgtcg gcgatggccg aggcgttgcc ggcggtcacg acgccgtcct
    15361 tcttgaagac gggcttgagg ccggcaagga tctccggcga ggtctcacgc gggcattcgt
    15421 cggtgtcgac caccttgggg tcgcccttgc gctgcgggat caccacgggg acgatctcgt
    15481 ccttgaacct gccgtccttg atcgccgcgg tcgcgcgcaa ctggctctgg tgggcgacct
    15541 cgttctgcag ttccttgctg atgccgaact gctcgctgag attctccgca gtgagaccca
    15601 tgtgggtgcc gccgatcgcg caccacaggc cgtcgagcac catcgcgtcg aacatctcct
    15661 gggacggcat gcccatgcgg tagccgctgc gaccgtcctg cagcagccac ggagcgcgcg
    15721 tcatgttctc cgtgccgccg acgaggtaca gctcgccgtc gccggccttg atgttggccg
    15781 ccgcccaggc aagcgcggag gcgctcgaca cgcagacgcg gttcagggtg atggccggga
    15841 cgctgtccgg gatgccggcc ttcagctcgg cctggcgggc gacgttctgc cccaggccgg
    15901 cttggatcac gttgccgaag atgacctcat cgagctggtc gccggtgacg cctgcgcgct
    15961 tgagcgcctc ggtcatggcg acggcgccga gctcctgcgc gggcgtgcct gcgaagcccc
    16021 cctggaagcg gccttgagcc gtgcggactg ccgagatgat gacgatgtcg ttgtcagaca
    16081 ctgcggcctc cttgagccga tgcgtgtgtg gctgcagccc ccgacgcgac tctaacgctc
    16141 tgcatcacga cacattgatg ggagtggttc atgagtatgc taccgggtca ggcgctctcg
    16201 tgcctgcgca cgacttcctg agcgaggaac gacgccactt cgagcggcgt cgtccgcggc
    16261 ccgaaccccg cgtcgtagcc gagctctttg gcgaacgcgt tgttgatccg cgggccgccg
    16321 acgatgagga cgaagcgctc gcgcacgccc tcggcctcga gcaactcgac gagctgcgtc
    16381 agatgctgga cgtggatgtt cttctgcgtg acgatctggc tgaccaggat ggcgtcggcg
    16441 ccgcgctcga gcgccgtccg caacagctgc gcgcagggga tctgcgaccc catgttgagc
    16501 gcgtccatct ccgggtagcg ctcgagcccg tagtttccgg cgaagccctt catgttcatg
    16561 atcgcgtcga tgcccacggt gtgggcgtcg ctttcgatcg ccgcgccgac gacgccgagc
    16621 ttgcggccga gcctctcctt gatcagcgcg ttgatcgcgt agtagtccat ctcctcgacc
    16681 tcgaggtagt cgcccttgac ctcgtcgggg tcgatgccgg tgtcggtgcg gccgtaggcg
    16741 acgaagaacg tgtagtcggg gtcgatggcc ttgcactcgg cgataccgac ggtgtggaaa
    16801 ccgagcttct ccaggaactg cttgccgacg tcggccgcct tgacggagta gggcatggga
    16861 agcgtgaagg agaactggac gacgccgtcg tcacgagtat caccatacgg tcttataagc
    16921 atcagtgccc cccttccgtc ttcgcggccg tgccgccggg gggcgggcct tcgggagcac
    16981 cgtccatcct gccgtctctc agcgccgcga agaacggatt gaagtagtcc ggcgtgacct
    17041 tgaagacgcc ctcgaagcca cggccgccct tcgggtcgcg cttcacgtcg gcgaactcgc
    17101 cgtcctcgag cgccctgaac aggctgatgt cgcgcacgtg ctcgagttgc tcggtggcct
    17161 tctggagctg atcgttggcg cgctgctcga tcatcccgcc gggcttgtag gtcacctcgc
    17221 cggcaaggtc gcggcagctc tcgaagacgt acttggcgtt cttgatgctc aggtagcggt
    17281 cctggagcag cggcgtgtga agggcttcgg tgagcatccc gagcagcatg atctcctggc
    17341 cggtgaagat gccggtgaag ttgaacatac cgtcgatgat gtgcccgtgg aagatgtccc
    17401 caggcatgta cttggtgggt ggcatgtact tgagcgggta gcccgggaac agctggcgga
    17461 cgagttgcgc gtcggcgatc tggtagacga cctggtcggg cgtctgcgga ttgatctcga
    17521 aggcgtgccc gaggccgatc tgccacggct cgaggttggc gcgcagggcg aaggcctcgt
    17581 tgatgaagtc agagctgacg accgtgtagc ccttctcgaa ggcgtccgcg gtagtcaggt
    17641 agttgtcctc accggtgttg atgatgatgc cggcgcgcgc gttgatcagg cggctgaggt
    17701 actggtcgac gaacgtccgc tgcatgttga tgttgcggaa gatgatgccg tacatggagt
    17761 cgttcagcat catgtcgaga cgctcgagcg cgcccatcgt ggcgatctcc ggcatgcaga
    17821 ggccgctggc gtaattgcac aggcggatgt agcggccgag ctcttcgccc acctcgtcga
    17881 gcgcggcgcg catcaggcgg aagttctcct gcgtcgcgta ggtgccgccg aagccttcgg
    17941 tggtctcgcc gaaggggacg tagtcgagca ggctctgcgc cgtcgtgcgg atgaccgcga
    18001 tcacgtcggc gccggcgcgg gcggccgcct tggcctgggc ggcgtcctcg tagatgtttc
    18061 cggtggcgac gatgacgtag acgtagggtt tgagcggctc gccggtctga gcgacgagct
    18121 cgttgcggcg cgcgcggacg gcgtcgatgc gcgcgacccc ttccctggcg agctcttcgg
    18181 cgcgcgcctc tgcggcggcg cgccgctccg cggggacctc agccagagcg aggtcgccgg
    18241 cggccaccgc ttcggccacc tgctgcgcgg ccttgccggt ctggttcatg aggtcgacga
    18301 acggtcgcat gatgccgccg ggcagcagct cgcgagcgtg ctctgcgacc acgttcacca
    18361 ggggtacgtc gatctcgttg acgccggcga tcccgaggag gcgcagggtg gctcgctcag
    18421 tggtgtcggt ggtgtgctcc tcgatgtgct gctggatgtc gtcggcgatc gcgttcgccg
    18481 cggtccgggc gcggtcgacc aggtcgccgt tcaatcggag ggcatcgttc acgtggccac
    18541 ccactttcgt caggaggagg gagaggactc ggggctcgcg cagccgccct ccggcagccg
    18601 ggagagcccg ttcacgacgt cgaacatcgg gacgcggccg ccgaccgcct cggcgaccga
    18661 ggagaagaag gccttggggc gatacggctg cggcacgcgg aaggggttca cggtgagcgc
    18721 gagcacgcgc agcggggtga gcgtctcgag gcggatgccg cggcgcagga agcggtagac
    18781 catggccggc ggcagcacca gcacggtggc gtcccgcacg accacgctga cctctgcgcg
    18841 tggcgacagc acgcgggtga agtcgtcggc gaactcctgg gtgagggcgc ctccgacgag
    18901 cagcgtgtcg gcgcccaggc gctcgacctc gcgcgcgacc gtgacgcctt cgcccaccac
    18961 gctggcgagc tcgagagtat gcgccagccc gtccggcgtg aaggcgacga cgcgcgagcc
    19021 tgcggccacg tgccgttcca tcagcgaccg gcgttgtgga gcgactcccg ggagcatgag
    19081 cagatcgagc gctccgaccg tcgtggcgag cacgtcgtcg agcgtgtcgc cgaccatgcc
    19141 cccggtggcc acgacgacgc cgtcgctgac gcgcggtgac gcgctgccga tgcggttgat
    19201 ggcgccgtcg atgatgacct ggtcggcgcc cagtgcctgc aggcgctcca cggtgacgcg
    19261 gagctccgcg agggtcgtcg gcccgctgac ctcgacggcg ccgtctccgg ccgcacgacc
    19321 gatgacgacc cggccgagcg gcgtgtggaa cggcagcacc tcgagtgtct ccatcgcgta
    19381 gcgcgagcgg tcgagcgagc cctgcgtcgt ggcgaccagc gtccccgccg gcggcgtgat
    19441 gctgggcttg gccaggccgg tgaggtggtc cgtgcgctcg ccgtcgaggc cgagcgaggt
    19501 gaggccgaac cgtcgcggcg agttggccat cagcgcattg accacggtgg tcttgccggc
    19561 gttcttgacg aggccgacga cagccagcga ccggctgccg gagccggcga tgatctcgta
    19621 gagggcgccg acggcagagc cgtcgggacg gaggccggtg gcgctcgtgc cgccgttgtg
    19681 ggtcaccgcc tgcctccggc cgcgcgcccg gggacggcga cgccggcatc acggccggcc
    19741 tgggcggcgg cgccgcgtcc ttcctcgccg tgacggatct ctttgaggta cgcgaagatc
    19801 gtgtagcgcg agaggtcgag cgcctccgcg acctgctccg ccgagcgctt gacgaggaac
    19861 gccccgcgct cttcgaggcg cttgaccacc tccatcttct cttcgcgcgc catggcggcc
    19921 ggcgtcttgc cgatctcgcc cagagtctgc gtgaccatgc ctgccaccac gtcgcggatg
    19981 tcgccggcga agatctcccc cagctcgggg ccaccgccgc ccaggcggcc tatcgccgtg
    20041 aagtcggcga gcacgcgctg ggcctgtacg acctcggaga cgtcgtagtt gaggccgagc
    20101 aggccgtacg gctcgccgtc cgcgccccgc agcgtgaccg cgagcgactt gaggatcttg
    20161 ccctcgtggg aggagacgta gaggcgcacg tcctgagcct ggtccgggct gcgcaggttg
    20221 cgcagcatcc ggtcggtggg cacgtcgcct acggtgcggc ccgacacgcc gccgttttcg
    20281 atcgccacga tggagtgggg gagccggctg acgtcgtgaa ggacgacctc gcaatcgggg
    20341 ccgaacatct gggcaaggcc ccggacgatg ggcagcagcg cctggaggcg ctcatcgagc
    20401 gggtgcgtga gcaaacgacc ctcctgggtc cgtgcctggc tgccggagac aaggcgtggg
    20461 cggcggaggt gtgctccacg ccgcttcccg gcctcgacac acggtataca cccgggcagg
    20521 gcggcctgtc aaaaacagtg tgccttctac ctgaaaaagt aggcacgagg cggagccggc
    20581 cgtctctcgg gctccgcctc gtgcctcggg tcgcaggggc caggcaccgc gtcgcccgcc
    20641 cctacatcag aggttgctgg gacgttccgc cagcgtgacg tcgacctcct cggtcttgcc
    20701 gtcacgctcg actgtcacgg tgacggtgtc gcccggcttc ttggtgccga gatacgcgat
    20761 caggtcgtcc gccgtcttga cctcggtgtt gtcgaccttg acgatgacgt cgccgccgat
    20821 ggtgaactgc tcgccctgca cggtcaccgt ctggtcgcca ccctggatgc cggccttgga
    20881 cgcagggctg tccggggaga ccgcctgcac cagggcgccg ccctgcgtct gcatgttgaa
    20941 catgcccgcg atgtcgctgg tgagggtctg gaggctgact cccatccagg cgtacgtcac
    21001 cttgccatcg gacttgatct gctccagcga gcggacggcc gtgttgatgg ggacggcgaa
    21061 gcccaagccc tggttgccgc cgcccgatgt ggcgatctgc tcgttgatgc cgatgacgtt
    21121 gccggcgctg tcgatgagcg gcccgcccga gttgccctgg ttgatcgcgg cgtccgtctg
    21181 gatgccgttc ggtatggtct gaccgttggg cgcctggagg ctgcgccccg tcgccgagac
    21241 gatgccggac gtgatggaga agtcgtagcc gagcgggttg ccgatggcca cgaccggctc
    21301 gccgacgacg acgtcgtcgg agtcgccgag cggaagaggc ttgaggtcga ccttggccgg
    21361 gtcgaccttg atgacggcga cgtcgctgcc gatgtccacg ccgaccagct ggccggggac
    21421 gcgttgcgtc tcggagccgc ccttgttgaa gaccaccgtg acgctgttcg cgcgctgtcc
    21481 gttctcgtcc acgacgtgcg cgttggtcag gatgtagccc tcactgtcga ccacgaagcc
    21541 cgtcccgagc gcctgcgcgc tctgaggctg tccgaactgg tcggtccccg atccgccgaa
    21601 ctccgccagg accatcacga cgccgcccga aagggtgctg tagatctgct ccggcgtgag
    21661 cccgccgccg gtcgtggacg cggatggggt gctgtagacc gtcggagtct caggcgttgc
    21721 ctggacgacc gtcttgctga ccgctccgat gtcgaacacg aacaccagga ggaggacgac
    21781 ggccgcggcg gctacggcgc cgcccgcgag gcccgccagg aaccctttga gcttcatgcc
    21841 tgcttccctt tcgctgtgct gcacagggag ggagacgcgc ccgccctggc cgatagtcat
    21901 ccgtaagatg ccgttcggct attacctgtc ccctacagga gtattaagca accgcacggc
    21961 gtggattaag gcgtgtgaac gcccccgctc cgttcggagg aggcacgacg acggcgcccg
    22021 ccggctgcac gcggcaggcg gacggtgaag cgcgcgccgc cgtccggcgg cgactccgcg
    22081 gtgatgctgc cgcccatcat ctcggtgagc tcgcgggcga tggagagccc gaggcccgcg
    22141 cccgcggtcg tcgcgcgctc gtcgcggccg cggtggaagc gctcgaacac gtgcggcagc
    22201 tcgctgcggg tgatgccggg gccgtcgtcg gcgacgacta cgacggcctc tttctcctcc
    22261 agtctcgagg agagtcgcac ggagctgccc gacggagagt acttgatcgc gttgtcgatg
    22321 agcgcgcgca gcacctgtac cagccgctgc tcgtcgcacc acgccgtttc gccctcctcg
    22381 cccctgaacg acagggcgac cgccttggtc tgcgcctgtg cctggaactc gtccagcacc
    22441 gaccggccga ccaccacgag gtccacgtcc tccgggttga gctcgaagga gcccgcctcc
    22501 acgcgggaga ggtcgagcat gctgacggcc atcgtgcgca gccggtcgac ctgctggcgc
    22561 atgatctgga ggaactcgcg ctgctcctcc gggctcagct cgccctcgtc gatcagctcg
    22621 agcgatcccg ccagtgagaa caacggcgtc ttgaactcgt gcgatgcatt ggcgatgagg
    22681 tcgcgacggg cgcgctcgag cctggcctgc gcagtcacgt cgcggagcac gacgatggag
    22741 gtgaagtcgg cgtcaccacc caccggatac gtggtcgcct ccagggtcac gtcgtcgtgc
    22801 gcgaacacca ccatgtcgaa atcagcgtcc cgttgcgagt cacgccacag ctgggcgacg
    22861 tcggccggga gggcgtcgtc gacggtctcc ccgaccagcg ccggtgcgtc gagcaggatc
    22921 gccgcggccg ggttggcgat ggtgactttg ccctcggctg agacgccgat cacgccctcg
    22981 ctcaggtcgt tgagcagtac ctcgacgcgg tcgcgctcgt actccacctg ggcgaaggcg
    23041 ctgcgcagcc tgtcggccat gacgttgaag gtgtcggcca gctgtccgat ctcgtcctcc
    23101 acggtctcgg ggaccttggc cgagaggtcg ccgccggcga tggcctccgc gctccgctcg
    23161 atgcgcttga ggcggcgggc gatgaagtac gaggccatgt agcccaggag caggctgatg
    23221 cccagcgcca gggccgtggc gaacaggagc tggcgctgca cggccgtcac ggcattgtcc
    23281 acatcctcga gcgactgcac cacgagtacg gcccccacgg cggtggtccc ggtgagcttc
    23341 gtgaacagcg gcaccgcggt cgcggcagcg cgcccggtgg ccgtggtgac cgtcccctgg
    23401 gtcacccgcc cggtgcggac ggtctcctcg agcatggggt agtcggacac gtccggcgct
    23461 gtctggctgc tcgagtcacg gaccgggacg aggctgcgcg cgtagatgat cgcccggccg
    23521 ctgaagcgcg ccccgatgat gttgagggtc tgggtcaccg catccgaatc gacgatgacc
    23581 agctgtccgc ggttggtgtc gtagctcatc agcccggggg cggtgagcgt ctggccgacc
    23641 agtcgggcgt tctccctcgc gtcgatcagc ttgcggtcgg tgagccgccc ctgaagcggc
    23701 ggcaccacgt acacccacgc gacgagcatc gccagcgcga cgagcgcgac gatgaggagc
    23761 gtgagccagt tcttgactga gctgaacatc aggtgagagg ctggcgcatc gggcgccgtt
    23821 gcctgcccta ttagaactcg cggaacttgt agccgatgtt acggaccgtg aagatgaact
    23881 cggggtcctt ggggtctgcc tcgagcttct cgcgcagatg gcggatgtgc acgtccacgg
    23941 tgcgcgaatc gcggtagtcg ctgtcgcccc acaccagctg cagcagcagc tggcggctgt
    24001 agaccctccc cggatgcgag atcagggtct tgaggatctc gaactcgatg taggtgaggt
    24061 cgaccttggc gccgcgcacg atgacgttgc gcttgaggac gtccacctcg aggtcgtcga
    24121 ccttgatgac ctccttcttg gtggcctcgg cgctgcggct catctcggcg cggcgcagct
    24181 gcgcgcgcac gcggctgcgg aactcgcgca ggctgaactt gtccttggtg atgtagtcgt
    24241 cggcgccgat ctcgagacca agcaccttgt cgaactcgtc gctcttggcg gtgagcatga
    24301 tgatcggcac gatgctttgg gcgcggatgc ggcggcagac ctccatgccg tccacgtgcg
    24361 gcagcatgat gtcgaggatg accaggtcga agtcctcgcg cgtgaaggtc tcgagcgcct
    24421 cgtcgccatc ccgtgcggtg acgacctggt agccctcctt ctcgagcggg aaccgcagca
    24481 gcttgaggat cgcctcctcg tcgtcgacga ggaggatgcg cgccccggac tcggccatca
    24541 gtggtctccc ttctcggcag ctggacgcgc gcccggggcc gcgtagccgc gggcccggaa
    24601 gcccgcaccc atcgcggccg cgatgtcggc gacgccgggc agatcggcgc ccgggtagtc
    24661 gacggcggtc agcgtggtct ccattccatg cgcaagacac tgttcggcga aacgcaggac
    24721 ggccctgtag gacttggtga agacgggccg gcacgcccgg tcgtactcct ccggggtcgc
    24781 cgcgttgagg ctgacggtga cggcgtcgag gccggccttc gccagggcgg ccggcacgtc
    24841 caggtcgggg ttgaggaggg cgccgtggcc gttggtgtcc agccgactgc ggatgcgccg
    24901 cagccgcagc cactcggtga cggcgagaac gacgtcgagc cgcatcgtcg gctcgccgaa
    24961 gccacagaac acgacctcgt cggcgggccc gtcgaggaag gcgacctcga tcgcctgcgt
    25021 gacctcctcg agctccggct cgcggtccag gcgcaggtcc tccccgaaca ccccgtcggt
    25081 ccactcgcgg aggcagaacg cgcaggagca ggagcagcgg ttggtgaggt tgagatagat
    25141 ggacctgccg gcgcgatagg cgatcgtccc ggcgaaagcg ggagtcgcgg tcatggcgtg
    25201 gcgggcggag gggtcatggt ggcgggccgc cgcggcctcg tcaacgctga ccgccggtcg
    25261 gcacgcagag tatagcaggg tagaatcggg agcccttgac cgacgtcacc gacagcctca
    25321 tcacgctctg cggccgcctg cgcgaggtcg tgctgccctc gctgggcacg cacgccgccc
    25381 gcggccacgc cggcgtggcg gtcggcggcg acgtcacctt cagcatcgac gaacaggcag
    25441 aggcgctcct ggtggagcac atgcgggagc accttccggc gtgggccttc tactccgagg
    25501 acggagggct gcagggcgcc tctgaccccg agctcatcct gatcgtggac cccatcgacg
    25561 gcacgcgccc ggcggcggcc gggttcgaga tggcgtgcgt cagcgtcgcc gcagtgcccg
    25621 caggcccggc gcccaccatg ggcggcgtgg tcgccggtgt catccaggag atcaagaacg
    25681 gcgacctctt cgtcgcggag aagggcgccg gctttcacat gaggcgcgcc gacggtgcgg
    25741 cgatcccctt tctgcccacg ccgcgctccg atctcgagag catcttctgg acgctcggat
    25801 tccgcggccg tccggccgtg atcctcgcga gcgtcctcga ggagctcatc gacgtctcgt
    25861 ccgtgggcgg ctgcgtcttc gacatcggct cggcgacgta cgggatcacg cgcatcctca
    25921 ccggccagct cgacgcgtac gtcgacatcg ggccggcgat catcgccgcg cacccgtgga
    25981 ccgaggccga gttccggcgc gtcgggcgag gcgccgtgct ctgcaactct ccctacgacg
    26041 tcgccgcggc gtacctgctg tgcaaggagg ccgggctgcc gttcggcgac gccgacggca
    26101 gttcgctcga cgacaggccg gtgctcggct cggacgcctc cttccagatg gccaccgtgg
    26161 ctgccggcaa ccaggagctg caggacgcgc tcgtcgagag cgtgcagcgc gggatcgccg
    26221 gcctgcgccg gccggaggcg tgagtcatgg ctgagaacct gaccgactgg cacgaggcga
    26281 tgctctacga gcagctcggc gagggcgtgg tgcagtgcca cgtgtgcccg cgcctctgcc
    26341 gcgtcgcgcc cggcaagagc ggcgtgtgcc gggcgcgggt caaccgcgac ggcgtcctct
    26401 acgccatcac ctatggcaag gtgtgcagcg tcgctgccga ccccatcgag aagaagccgc
    26461 tctaccattt cttccccggc accacggtgc tctcgctggg cagcctgggc tgcaacttcg
    26521 cctgccggca ctgccagaac tggcagatcg cgcacgcgga cccgggagag gcgacgcgcg
    26581 acctgcgcga cctgcccatc cgcaatctcc ccgtgctggc cgcgaacaac gagtgccagg
    26641 gcgtggcctg gacctacaac gagcccacga tctggctgga gtacatcttg gacggcgccc
    26701 aggcggcgca cgagcatggg ctgtacacgg tgatggtcac caacggctac atcaccgatg
    26761 acgccctctc gatcctcggg ccgcacatcg acgcctaccg cgtcgacgtg aagagcttca
    26821 ccaatcagca gtacaaggag ctgt
//
LOCUS       DASUMX010000087         6497 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1173233, whole genome shotgun sequence.
ACCESSION   DASUMX010000087 DASUMX010000000
VERSION     DASUMX010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 6497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6497
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1173233"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..123
                     /locus_tag="VFZ86_04210"
     CDS             <1..123
                     /locus_tag="VFZ86_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002381601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641528.1"
                     /translation="RKLLEKQKEGKKRMKQLGVVEVPQEAFLAVLELEPGDKDR"
     gene            147..524
                     /locus_tag="VFZ86_04215"
     CDS             147..524
                     /locus_tag="VFZ86_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012893412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641529.1"
                     /translation="MDATLARKLQLKPGQVVRALHVPEKMELDLPEAGVAEAAGGHAP
                     APATLVFVRTHDEAEAHTGAAVEAALADELSWVAYPKAGKLGTDLNRDSLAAFMTARG
                     VRPVRQVAIDDTWSALRFRPPSP"
     gene            521..1087
                     /locus_tag="VFZ86_04220"
     CDS             521..1087
                     /locus_tag="VFZ86_04220"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018797242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5641530.1"
                     /translation="MSAPPARFARPDASFRDSWLEALREYHDEGRHDELDEDLLADRE
                     AFARYCDALNAAAVQPGEPDVYLAELRGTAPRGEWRDGYVPYTVLWWTAGHDYLGRLT
                     IRHRLTPHLLYHGGNIGFEVRPSARRRGHATAMLAAALPLAAALGIDPARLDCEAGNV
                     ASRRVIEANGGLLDEERDGSLFFWAPTR"
     gene            1084..2295
                     /locus_tag="VFZ86_04225"
     CDS             1084..2295
                     /locus_tag="VFZ86_04225"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen-III oxidase family protein"
                     /protein_id="HEX5641531.1"
                     /translation="MTDLFQGPVRHVYVHVPFCRAKCDYCAFASLPVGADGEDGRAGA
                     QAARLLDTYVDGVAAEWERERAAHDVRRLHTLYLGGGTPSLLGPDRLERLLELFRPHL
                     TPHAEVTVETNPEDVDGPYAAWAAGAGVRVSLGAQSFSAQLRAALGRTARSDPAAAFR
                     RLRSAGVANAGLDLIHGIPGQTPELLCADIAAVLDLRPDHVSWYELDVIEGTALAARL
                     AGDAASVTQAIDPAAMPPDPMDDTRATGYRRIVRELSRAGYDWYEVSNFALPGRRARH
                     NVAYWRARPYLGLGPGAVSTVGARRWTNAADPSAWAAALARGASPPRTVEALDAPTRA
                     RERLLLAARCGERVPLAEVAAVLDRDVAASLAAAGLVSLHSGTIRVTRKGRHVADGLC
                     VRLFRSPHLRG"
     gene            2297..3316
                     /gene="hrcA"
                     /locus_tag="VFZ86_04230"
     CDS             2297..3316
                     /gene="hrcA"
                     /locus_tag="VFZ86_04230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEX5641532.1"
                     /translation="MPLTARQEQVLRTVIEEYIALGAPVGSRHLSERFHFGVAASTIR
                     NDLADLEDMGMLAHPHTSAGRVPTDAGYRHYADIVAAERREGTPALLLERDRAASEID
                     DALRETAEALSRVTELLAVVSAPAVSTSSLRHIEILALQPRLVMVVLITTSGQVTKRV
                     FSFADPVDEGLAEFARVYLNERLVGAQLGTRRVAAVFESPDLRPKELAFLEVLRPAFE
                     APGPGDVEGLHLGGTSRLLDNLTRAGAGHLNEVLQMLESRYNLLELLAGALREEGMYL
                     RIGHEIASPSLQECSLVATTYGVANRSLGTVSVIGPTRMDYQRVIATVRATADGLSSY
                     LEDIW"
     gene            3339..4487
                     /gene="dnaJ"
                     /locus_tag="VFZ86_04235"
     CDS             3339..4487
                     /gene="dnaJ"
                     /locus_tag="VFZ86_04235"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEX5641533.1"
                     /translation="MKRDYYDVLGVRRDADAGDIKKAFRKLARECHPDVNQEDPEAEA
                     QFKEAAEAYEVLSDADSRAAYDHYGFDGLRGRPMTDFEHVGFGDLFNIFFGGGMFDSV
                     LRDAGGMWGAAGGSSPAPRGDDVEAQVTLTFAEAVFGVTKDVEVTADAACETCSGSGA
                     KAGTGREQCPQCHGSGRMREVSSLGGFGQFIRTSTCNICRGQGTIVKEPCPTCLGSGR
                     VRKTHTVPVEVPAGISPGQRLRLAGEGGAGAHGGRPGDLYVAVAVEPSEDFVRDGDDL
                     IYRLDITMVEAALGTTAYVPALDGDIEVQLKPGTQPGEVKSYRSRGVPVLQGYGRGDL
                     KVVVNVLVPRKLTDEQRELLEQFAELSGEATYEVDQSFLDKVRAVFRQ"
     gene            4484..5440
                     /locus_tag="VFZ86_04240"
     CDS             4484..5440
                     /locus_tag="VFZ86_04240"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEX5641534.1"
                     /translation="MKRPGYTRYALRLVKPAGARPTVAPVTGAGDARADQRGSVDDWS
                     GAAAYERGPDAQALDYADPRAVAEIRALQPAGWQEEDGGGTLVFWLEDGAEADAEVAA
                     ALKRLGASGRLEVAREQPGWEDAWRRFHRPHVIGRLYVRPPWYPARDDLLDIAVEAGL
                     AFGTGGHASTRQCLEFVQAVTPGSLLDLGSGSGVVSFAALRLGFAPVTGIDIDPVAVH
                     AAAGNAAMNGLAPTFLVGDATDPSYPLPGADVVVANIALRPILRLAERWRARGTAPPA
                     ELLLSGLLVEQAEEALAAHPGFTESARADDGTWLTLRLTRRA"
     gene            5437..>6497
                     /locus_tag="VFZ86_04245"
     CDS             5437..>6497
                     /locus_tag="VFZ86_04245"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX5641535.1"
                     /translation="MTRVAASFVGCKVSQADGEEAPAGLAAAGLEPVTARDAADVVVV
                     HTCCVTAEAERKSRRLVRRAGAGGRRVVVAGCAAALRPEQFEGEGVTVAARPDWAELA
                     GRLAGEDVRRRAAGAAGGATETGATHDAAHDAAHDAASDATPCATQDTPARDTAGSAA
                     ARDTAVAPAARDLAAPRATPGARTRLVLKVQDGCSGACAYCVVRLVRGRPRSLPLARA
                     LTKARAGLDAGGGEVVLSGIDLGAWRDGAARLPDLVAAVADLDGVRRVRLSSVEPRHV
                     DAALLEQLAHPRVARHLHVPLQSADDGVLAAMRRPYTWERYLRAVDGLRDVPGGAMLS
                     TDVIVGYPTEDEAAFARTL"
BASE COUNT          845 a         2397 c         2416 g          839 t
ORIGIN      
        1 cgcaagctgc tcgagaagca gaaggaaggc aagaagcgga tgaagcagct cggcgtggtc
       61 gaggtgccgc aggaggcctt cctcgccgtg ctggaactcg agccgggcga caaggaccgc
      121 tgacgccgac cagacggagg ccgccgatgg acgccacgct ggcgaggaag ctgcagctca
      181 agcccgggca ggtggtgcgg gcgctgcacg tcccggagaa gatggagctc gacctgccgg
      241 aggccggcgt ggccgaggcc gcgggcgggc atgcgccggc tccggcgacg ctcgtgttcg
      301 tgaggaccca cgacgaggcc gaggcacata ccggcgccgc tgtggaggcg gcgctcgccg
      361 acgagctctc gtgggtcgcg tatcccaaag ccggcaagct cggcaccgac ctgaatcgcg
      421 actcgctggc ggcgttcatg accgcccgcg gtgtgcggcc cgtccgccag gtggcgatcg
      481 acgacacctg gtccgccctg aggttcaggc cgccttctcc ttgagcgcgc cgccggcccg
      541 gttcgcgcgc cccgacgcga gctttcgcga ctcctggctg gaggcgctcc gcgagtacca
      601 cgacgagggc cggcacgacg agctcgacga ggatctcctc gcggaccgcg aggcgttcgc
      661 gcggtactgc gacgcgttga acgccgccgc cgtgcagccc ggcgagcccg acgtctacct
      721 ggcggagttg cgcggcactg cgccgcgggg ggagtggcgg gacggctacg tgccctacac
      781 ggtgctgtgg tggacggccg gccacgacta cctcggccgc ctcacgatcc gccaccggct
      841 gacgccgcac ctgctgtacc acggcggcaa catcggcttc gaggtgcggc cgtcggcgcg
      901 gcggcgaggc cacgccacgg ccatgctggc cgcggcgctg ccgctcgccg ccgccctcgg
      961 catcgacccg gcgcgcctcg actgcgaggc gggcaacgtc gcctcgcgcc gggtgatcga
     1021 ggccaacggc ggcctgctcg acgaggagcg cgacggcagc ctcttcttct gggcgccgac
     1081 gcggtgaccg acctgttcca ggggcccgtg cggcacgtct acgtgcacgt gccgttctgt
     1141 cgtgccaagt gcgactactg tgcgttcgcc tcgctgccgg tgggcgcgga tggcgaggac
     1201 gggcgggccg gggcgcaggc cgcccgcctg ctcgacacct acgtggacgg cgtcgccgcc
     1261 gagtgggagc gggagcgggc ggcgcacgac gtgcgccgcc ttcacacgct ctacctcggc
     1321 ggcggcacgc cgagcctgct ggggccggac cggctcgagc ggctgctcga gctcttccgg
     1381 ccgcacctca cgccgcacgc cgaggtcacc gtcgagacca accccgagga cgtcgacggc
     1441 ccgtacgcgg catgggcggc gggagcgggc gtgcgcgtct cgctcggcgc gcagagcttc
     1501 tccgcgcagc tgcgcgccgc cctcgggcgg acggcccgat cggatccggc cgccgccttt
     1561 cgccggctgc ggtcagccgg tgtcgcgaac gccgggctgg acctcatcca cggcatcccc
     1621 ggccagacgc cggagttgct ctgcgcggac atcgccgccg tgctcgacct gcgcccggac
     1681 cacgtctcct ggtacgagct cgacgtgatc gaggggacgg cgctggcggc gcggctggcg
     1741 ggggacgccg cgtccgtcac gcaagccatc gatccggcag ccatgccgcc ggaccccatg
     1801 gacgacaccc gcgcgaccgg ctaccggcgc atcgtacggg agctctcacg cgccggctac
     1861 gactggtacg aggtcagcaa cttcgctctg ccggggcgca gggcgcgcca caacgtggca
     1921 tactggcgcg cccgcccgta cctcggcctc ggccccggcg ccgtgagcac cgtcggcgca
     1981 cggcgctgga ccaatgcggc cgacccgtcg gcgtgggcgg cggcgctggc gcgcggcgcg
     2041 tcgccgccgc gcacagtgga ggcgctcgac gcgcccacac gcgcccgcga gcgcctcctg
     2101 ctggcggcgc gctgcggcga gcgcgtcccc ctcgccgagg tggccgcggt cctcgaccgc
     2161 gacgtcgccg cgagcctcgc cgccgctgga ttggtttccc tgcacagtgg tacaatccgc
     2221 gtgacgcgaa aggggaggca cgtggcagac ggactgtgcg tgcgcctgtt tcgctctccg
     2281 catttaaggg gttaggttgc cgctcacggc gaggcaagaa caggtcctgc ggaccgtcat
     2341 cgaggagtac atcgcgctcg gcgctccggt cgggtcgagg cacctcagcg agcggttcca
     2401 cttcggcgtg gccgcctcca ccatccgcaa cgacctggcc gacctcgagg acatgggcat
     2461 gctggcgcac ccgcacacct ccgcggggcg ggtgcccaca gacgcgggct accggcacta
     2521 cgccgacatc gtcgcggcgg agcggcggga gggcacgccg gcgctgctcc tcgagcgcga
     2581 ccgcgcggcc agcgagatcg acgacgcgct gcgcgagacc gccgaggccc tctcgcgcgt
     2641 caccgagctg ctcgccgtcg tctcggcgcc ggccgtcagc acctcgagcc tgcgccacat
     2701 cgagatcctc gccctgcagc cgcgcctcgt catggtggtg ctcatcacca cctcggggca
     2761 ggtcacgaag cgggtgttct cgttcgccga cccggtcgac gaaggcctcg ccgagttcgc
     2821 acgtgtgtac ctcaacgagc ggctcgtggg ggcgcagctg ggcacgcgcc gcgtcgccgc
     2881 cgtgttcgag agccccgact tgcgacccaa ggagctggcc ttcctcgagg tgctgcgccc
     2941 ggcgttcgag gcgcccggcc cgggcgacgt ggagggtctg cacctgggcg gcacgtcgcg
     3001 cctcctcgac aacctcaccc gcgccggggc cggtcacctc aacgaggtgc tgcagatgct
     3061 cgagtcgcgg tacaacctgc tggaactgct ggccggggct ctgcgcgaag aaggcatgta
     3121 cctgcgcatc gggcacgaga tcgcgtcgcc gtccctgcag gagtgctcgc tggtcgcgac
     3181 cacctacggc gtggccaacc gcagcctcgg cacggtgagc gtcatcggcc cgacgcgcat
     3241 ggactaccaa cgtgtgatcg cgacggtgcg cgccacggcc gacggcttga gctcgtatct
     3301 cgaagacatc tggtgaccac ctccgactgg ggcgaccatt gaagcgtgac tactacgacg
     3361 tgctcggcgt gcgccgcgac gccgacgccg gtgacatcaa gaaggccttc cgcaagctgg
     3421 cgcgcgagtg ccacccggac gtgaaccagg aggaccccga ggccgaggcg cagttcaagg
     3481 aggcggcgga ggcctacgag gtgctctccg acgccgattc gcgcgccgcc tacgaccact
     3541 acggcttcga cggcctgcgc gggcgcccga tgaccgactt cgagcacgtc ggcttcggcg
     3601 acctcttcaa catcttcttc ggcggcggca tgttcgactc ggtgctgcgc gacgccggag
     3661 ggatgtgggg cgcggcaggc ggcagttcgc ccgcgccgcg cggcgacgac gtcgaggccc
     3721 aggtcacgct caccttcgcc gaggcggtct tcggcgtgac caaggacgtg gaggtcaccg
     3781 ccgacgccgc gtgcgagacc tgctccggca gcggcgccaa ggccggcacc ggccgtgagc
     3841 agtgcccgca gtgccacggg agcggccgca tgcgcgaggt ctccagcctc ggcgggttcg
     3901 gccagttcat ccgtaccagc acctgcaaca tctgccgcgg ccagggcacc atcgtcaagg
     3961 agccgtgccc gacgtgcctc ggctcgggtc gcgtgcgcaa gacgcacacc gtgcccgtgg
     4021 aggtgccggc cggtatctcc ccgggccagc gcctgcgcct ggcgggcgag ggcggcgccg
     4081 gcgcccacgg gggcaggccc ggcgacctgt acgtcgccgt cgcggtcgag ccgagcgagg
     4141 acttcgtgcg cgacggcgac gatctcatct accgcctcga catcaccatg gtcgaggcgg
     4201 cgctcggcac caccgcctac gtgcccgccc tcgacggcga catcgaggtg cagctcaagc
     4261 cgggcacgca gcccggcgag gtgaagtcgt accgcagccg cggcgtgccc gtcctccagg
     4321 gctacgggcg cggcgacctc aaggtcgtgg tcaacgtgct cgtcccgcgc aagctcaccg
     4381 acgagcagcg tgagctgctc gagcagttcg ccgagctcag cggcgaggcg acgtacgagg
     4441 tcgaccagag cttcctcgac aaggtccgcg ccgtctttcg tcagtgaagc gccccggcta
     4501 cacgcgctac gccctgcggc tggtgaagcc ggcgggggcg cgtcccacgg tcgcgccggt
     4561 cacaggggcc ggtgatgcgc gtgcggacca gcggggatcc gtcgacgact ggtccggagc
     4621 ggcggcctac gagcgcggcc cggacgcaca ggcgctcgac tacgccgacc cccgggccgt
     4681 cgcggagatc cgcgcactgc agcccgccgg ctggcaggag gaggacggcg gcggcacgct
     4741 cgtcttctgg ctcgaggacg gcgccgaggc cgacgccgag gtcgcggcgg cactcaagcg
     4801 gctcggcgcg tccggccgcc tcgaggtcgc ccgcgagcag cccggctggg aggacgcctg
     4861 gcggcggttc caccgcccgc acgtcatcgg acgcctgtac gtgcgcccgc cgtggtaccc
     4921 ggcccgcgac gacctgctcg acatcgccgt ggaggccggc ctcgcgttcg gcaccggcgg
     4981 gcacgcgagc acgcgccagt gcctcgagtt cgtccaggcc gtcaccccgg gctcgctcct
     5041 cgacctcggg tcgggctccg gcgtggtctc cttcgcggcc ctgcggctcg ggttcgcgcc
     5101 cgtcaccggc atcgacatcg accctgtcgc cgtgcacgcc gccgccggca acgcggccat
     5161 gaacggcctc gcgccgacgt ttctggtggg cgacgccacc gacccctcgt acccgctgcc
     5221 cggcgccgac gtcgtggtgg ccaacatcgc gctccgcccg atcctgcggc tggcggagcg
     5281 ctggcgtgcg cgcggcaccg ccccgccggc ggagttgctg ctctcgggcc tgctcgtcga
     5341 gcaggcggag gaggcgctcg ccgcgcaccc cggcttcaca gagagcgcgc gcgccgacga
     5401 cggcacctgg ttgacgctgc ggctcacgag gcgggcgtga ccagggtcgc cgcgagcttc
     5461 gtgggctgca aggtgagcca ggccgacggg gaagaggccc cggcggggct cgccgccgcc
     5521 gggctcgagc cggtgacggc tcgggacgcg gccgacgtgg tcgtggtgca cacctgctgc
     5581 gtgaccgccg aggccgagcg caagtcccgc cggctcgtcc ggcgcgcggg cgccggcggc
     5641 aggcgcgtgg tggtggccgg gtgcgcggct gcgcttcgcc cggaacagtt cgagggcgag
     5701 ggcgtgaccg tggcggctcg cccggactgg gcggagctgg ccgggcggct ggccggggaa
     5761 gacgtgcgcc gccgcgccgc cggggccgcg ggtggtgcga ccgagactgg cgccacccat
     5821 gacgccgccc atgacgccgc ccatgacgcc gccagtgatg ccactccctg cgcgacacag
     5881 gacaccccgg ctcgggacac tgcaggctcc gccgcggctc gggacactgc agttgccccc
     5941 gcggctcggg atctggcggc gcctcgggcg accccgggcg cacgcacgcg cctcgtgctc
     6001 aaggtccagg acggctgctc cggcgcctgc gcgtactgcg tcgtgcggct ggtgcgcggg
     6061 cggccgcgca gcctgccgct cgcgcgggcg ctcacgaagg cccgcgcggg actggacgcg
     6121 ggcggcggcg aggtggtgct gagtggcatc gacctcggcg cctggaggga cggcgcggca
     6181 cggctgcccg acctggtcgc cgcggtcgcg gacctcgacg gggtgcggcg cgtgcgcctg
     6241 tcctcggtcg agccgcggca cgtcgacgcc gcgctgctcg agcagctcgc gcacccgcgc
     6301 gtggcgcggc acctgcacgt gccgttgcag tcggccgacg acggcgtgct cgcggcgatg
     6361 cgccgtccct atacctggga gaggtatctg cgcgcggtcg acggcctccg ggacgtgccc
     6421 ggtggggcga tgctgagcac agacgtcatc gtcgggtacc cgacggagga cgaggcagcg
     6481 ttcgcgcgca cgctgga
//
LOCUS       DASUMX010000088        11449 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_23956, whole genome shotgun sequence.
ACCESSION   DASUMX010000088 DASUMX010000000
VERSION     DASUMX010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 11449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11449
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23956"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VFZ86_04250"
     CDS             <1..213
                     /locus_tag="VFZ86_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX5641536.1"
                     /translation="AAAVAGLLARADVYLVSEFPDAVVRSMCMTPFASVDGAVRAALD
                     RTGPGARCLVVPHGSRVTAAGSGLPE"
     gene            complement(258..941)
                     /locus_tag="VFZ86_04255"
     CDS             complement(258..941)
                     /locus_tag="VFZ86_04255"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX5641537.1"
                     /translation="MYDWRADWQTRFGRTEPKIVCVGLNYTDHAAEQGARLPANPMLF
                     AKFANALCGDGGDIILPPGIGHVDAEAELCVVIGREARDVPADDALDVIHGYLCGNDV
                     SARDLQFGDKQWFRGKSQDTFCPIGPGIVPVAELGDAGDLRVMQRLNGQTLQDSRTGS
                     LIFDVRTLVAFISHALTLLPGDLIMTGTPEGVGVFRDPKLSLQHGDVVEVEVEGVGVL
                     RNPVREAAR"
     gene            complement(1106..2362)
                     /locus_tag="VFZ86_04260"
     CDS             complement(1106..2362)
                     /locus_tag="VFZ86_04260"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEX5641538.1"
                     /translation="MDHVHNEPEPRVISSASGQPAELRRVERKMRIHRQPKTLITGVG
                     AVDQLGVEAKKLNGSKVLVITDQGVSECGMCIDVERPLAEAGLEVDFYDKAVPEPPMS
                     TIEEIVRIVEKGGYDLIVGFGGGSPIDVAKAAAVLPHTGDTVYDYVGIDQVKKKGLPI
                     IAIPTTSGTGSECTAIAIFANEKLNVKQGLVSPFLMPNVALIDPALTLTCPPNVTAAS
                     GMDALIHNIEAYISVNATMHTDSLAYEGIKLISQSIRTAVYDGTNMWARDNMAMGSMM
                     GGIAFGNAGVGAVHALSYPIGGMFHVPHGVANTMVLPWAMDFNKLACLRWFRQFGEAM
                     GENMEGLSDREAADKTVAALRLLADDLGVPQYLSDVDIPESAIPDLVEGAKTQARLWN
                     NNPRKFTPEDMEQVYRDMAVRPGAKA"
     gene            complement(2368..3699)
                     /locus_tag="VFZ86_04265"
     CDS             complement(2368..3699)
                     /locus_tag="VFZ86_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HEX5641539.1"
                     /translation="MDWKTDYAGKRTTAAEALACVKSGDRVVFAHACGEPLDLVDALV
                     ARSGELERVEINHMVAMGRGEYCRPEYADSFYHKSLFVGASSREAVKDGRGDYVPVFF
                     SEIPKLFCEGYLTPDVVLIQVSPPDKHGFCSCGISVDYTKPAAQVAGRVIAQVNPKMP
                     RTHGDSFLHVSDIDAFVESDQDIIELQPPRIGPVEEAIGKHIAGLVDDGSCLQLGIGG
                     IPDAVLLFLHEKNDLGIHSEMFSEGVVDLYNEGVITNARKAYHPGKMVATFLMGTRRL
                     YDFVDDNPVVNMLPVNIVNDPFVIGQNDRVVAINSAIQVDLMGQVVADSMGAAQFTGI
                     GGQVDFVRGAARSRDGKAIIALPSTAKKGSVSRIVAALAPGTAVTTMRADVDYIVTEH
                     GAAHLRGLGLRERAEALIEIADPAFRDQIREEAAEVYGVGRLPRQPLTKES"
     gene            complement(3705..4064)
                     /locus_tag="VFZ86_04270"
     CDS             complement(3705..4064)
                     /locus_tag="VFZ86_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641540.1"
                     /translation="MAVVYCGGCNPHIDRAAVAAGLPVDDPDVRPGTTVLLSGCPRAC
                     ASDHMLVAEGDKRCAYGTPLEEPATPLYGQAGAGAEGAAAGPDGPPVVVVAGELVDGV
                     PTPSEELVPAVTRKLKE"
     gene            complement(4099..5595)
                     /locus_tag="VFZ86_04275"
     CDS             complement(4099..5595)
                     /locus_tag="VFZ86_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase family
                     protein"
                     /protein_id="HEX5641541.1"
                     /translation="MALKTPEEFVQSLADLHLQIYLFGEKIDDYVNHPLIRPSINCIA
                     TTYEMAAQPEYEDLMLATSHLTGKKVNRFTHIHQSTDDLSKKVKMQRLLGQKTGSCFQ
                     RCVGMDAINALYSVTYEIDQARGTDYHQRFTKYVQYLQDEDIVADGAMTDPKGDRSLP
                     PHQQPDPDVYLHIVEKRDDGIVVRGAKAHQTGACNSHEIIVMPTVAMREGDEDYAVSF
                     SCPANAEGVYYIYGRQSCDTRKFEESDLDVGNMRYGGQEALMVFDDVFVPWERVYMCG
                     EIEFSGALVERFAGFHRQSYGGCKVGVGDVLIGAAALAADYNGAARASHIKDKLIEMT
                     HLNETLFSCGLACSYEGVKMPAGNYQIDMLLANVCKQNVTRFPYEIARLAEDIAGGLM
                     VTMPSAQDLASEKLGPIVEKFLAGQCDTPTIDRMRVLRLIENLTIGAGAVGYRTESMH
                     GAGSPQAQRIMISRQSNLNHKKELAKAIAEVGKPWDKTKVKAPTAVAQ"
     gene            6103..7464
                     /gene="gdhA"
                     /locus_tag="VFZ86_04280"
     CDS             6103..7464
                     /gene="gdhA"
                     /locus_tag="VFZ86_04280"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEX5641542.1"
                     /translation="MSAFVDEFMAELTAKNPGEPEFHQAVLEVAESLEVVLDKNPIYR
                     KMKVLERIAEPERVFLFRIPWVDDSGEIQINKGYRIQMNSAIGPYKGGMRLHPSVNLG
                     VLKFLAFEQVFKNSLTTLPMGGGKGGSNFDPKGKSDAEVMRFCQSLMTELCKYIGQFT
                     DVPAGDIGVGGREIGFMFGQYKRIRGEFVGVLTGKGRNWGGSLIRPEATGYGAVYFAA
                     EMLTTRGETLEGKTCLVSGSGNVAQYATEKILDLGGKPVTLSDSGGYIYDEAGIDREK
                     LAFVLELKNVKRGRISEYADKFKSAVYTPVDPALDHNPLWGHKAECAFPCATQNEINA
                     KDAATLVDNGVYVVSEGANMPTVPDGVNLFVEKKLLYGPGKAANAGGVATSGLEMSQN
                     SLRLSWTREEVDARLHGIMKAIHKQCTDAAEAYGMKDNYVAGANIAGFLKVADTMLDH
                     GVV"
     gene            7560..7901
                     /locus_tag="VFZ86_04285"
     CDS             7560..7901
                     /locus_tag="VFZ86_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003622497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEX5641543.1"
                     /translation="MKKIECYIQPYNLDAVAEALAVEGVVGMSVAEVKGFGVQKGFVR
                     GEDVKPGEYRFHPKMKVEMVVLEKDVERIITAINASLKSNQIGEGKIFVLPIDDAVRT
                     RTGERGRDAIT"
     gene            complement(8064..8384)
                     /gene="trxA"
                     /locus_tag="VFZ86_04290"
     CDS             complement(8064..8384)
                     /gene="trxA"
                     /locus_tag="VFZ86_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017539605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX5641544.1"
                     /translation="MADVKTLTDATFDTEVKDAGTPLIVDFWAPWCGPCRMVGPVIDE
                     IAQEHGDKVSVGKLNVDENPATAQKYGIMSIPTIILFKDGEPAKKVIGARSKGDFLKE
                     FELA"
     gene            complement(8442..8834)
                     /locus_tag="VFZ86_04295"
     CDS             complement(8442..8834)
                     /locus_tag="VFZ86_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HEX5641545.1"
                     /translation="MESVEYGVGRYLDEPYESALPRVVEALKAEGFGVITEIDVRQTM
                     KDKLDVDVPPHTILGACNPKLAHAALEVEPDLGVLLPCNVVVYATDQGTRVTAVNAGA
                     MLGMVGNKDLEPIAVQVQERLDRVLGSL"
     gene            9027..10685
                     /gene="ilvD"
                     /locus_tag="VFZ86_04300"
     CDS             9027..10685
                     /gene="ilvD"
                     /locus_tag="VFZ86_04300"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEX5641546.1"
                     /translation="MRSDEIKQGLARAPHRSLLRALGVSDAEMERPFVGVVNAFSEVV
                     PGHLHLRSVVDAVKGGVRAGGGVPMEFGVMGICDGLAMNHRGMLYSLPSRELIADTVE
                     SMTIAHAFDALVLVPCCDKIVPGMLMAAMRLNVPAIMVGGGAMLAGHLEGRTLDLNSV
                     FEAVGRHQAGELDDESLKAIECNACPTCGSCSGMFTANSMNCLAEVIGMALPGNGTIP
                     AVYSERIRFAKDSGEQVMRVLAADRKPRDILTEAAVGNALAADASLGCSTNTVLHLAA
                     IATEAGLDFDLRRINEVAAKVPHICKIAPAGTHHMEDLYHAGGIQAVMNRLIGAGLMD
                     GSALSVAGGTVGEAVAKARVLDDAVIRPVDDPWHATGGLAVLFGNLAPAGAVVKEGAV
                     ADEMLVHSGPARVFESEADAVAAIAARDIADGSVVVIRSEGPKGAPGMPEMLSATSML
                     AGQGRDKDVALITDGRFSGATRGAAVGHVSPEAHAGGPIAAVRDGDTISIDIPARTIT
                     LELPEEEIARRLADWRPAPRDGVTGYLARYVKLVSGAEKGAVLQ"
     gene            10682..>11449
                     /locus_tag="VFZ86_04305"
     CDS             10682..>11449
                     /locus_tag="VFZ86_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009454838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX5641547.1"
                     /translation="MTPDRRVKGSQALVAALEHEGCKVLFGYPGGVAIPFYDALYDAK
                     ELRHVLVRHEQGAAHAADGYARSTGKVGVCTATSGPGATNLVTGIATAYLDSSPLVAI
                     TWQVRSDLIGTDAFQEADITGITLPIVKHSYLVKDPAELPQTVHEAFHIASTGRPGPV
                     VIDIPVDIALGELAYRDADTLDLPGYKPTFKGHIKQIRAAARAINEAQRPVLYLGGGA
                     ITSDAAPEILKLAELRRIPVATTLMALGAFPETHDLSM"
BASE COUNT         1713 a         3905 c         3997 g         1834 t
ORIGIN      
        1 gcggccgcgg tcgcggggct cctggcgcgc gccgacgtct acctggtctc ggagttcccc
       61 gacgccgttg tgcgcagcat gtgcatgacg ccgttcgcga gcgtcgacgg cgcggtccgc
      121 gccgccctcg accgcaccgg ccccggcgcc cgctgcctgg tggtgccgca cgggagccgc
      181 gtgaccgccg cgggctccgg ccttccggag taggtcggcg ggagtccgcc ggccgggctg
      241 agcgctcccg gctgccgtca gcgggctgcc tcccggaccg ggttgcgcag cacgccgacg
      301 ccctccacct cgacctcgac gacgtcgccg tgctgcagcg agagcttggg gtcgcggaac
      361 acgcccacgc cctcgggggt gcccgtcatg atgaggtcgc cgggcagcag cgtgagcgcg
      421 tgagagatga acgccaccag ggtgcgcacg tcgaagatga ggctgccggt gcgcgagtcc
      481 tggagcgtct ggccgttcag tcgctgcatc acgcgcaggt cgccggcgtc gccgagctcg
      541 gcgaccggca cgatgcccgg tccgatgggg cagaaggtgt cctggctctt gccccggaac
      601 cactgcttgt cgccgaactg caggtcgcgc gcgctcacgt cgttgccgca caggtagccg
      661 tggatgacgt ccagcgcgtc gtccgccggc acgtcgcgcg cctcgcggcc gatgacgacg
      721 cacagctccg cctcggcgtc cacgtggccg atgccggggg gcaggatgat gtcgccgccg
      781 tcgccgcaga gcgcgttggc gaacttggcg aagagcatgg ggttcgccgg cagcctggcg
      841 ccctgctcgg cggcgtggtc cgtgtagttg aggccgacgc acacgatctt gggctcggtg
      901 cgcccgaagc gagtctgcca gtctgcgcgc cagtcgtaca tgcgaggtcc tccgtcggcg
      961 gtcgcgggaa actgacctca tggtatgcga aagggcgggc gcgccgctct cgcgtcgcgc
     1021 ccgccccgtg cgctggacgc cgccccccga cggcggccgg cgctcccccc gtccttgagc
     1081 gtcggacggc cgtaggctcc cgggcctagg ccttcgcgcc cggccggacg gccatgtcgc
     1141 ggtagacctg ctccatgtcc tcgggcgtga acttgcgcgg gttgttgttc cacaggcgcg
     1201 cctgcgtctt ggcgccctcc acgaggtcgg ggatcgccga ctcggggatg tccacgtcgc
     1261 tgaggtactg cggcacgccc aggtcgtcgg cgagcagacg cagcgcggcg accgtcttgt
     1321 cggccgcctc gcggtcggag aggccttcca tgttctcgcc catggcctcg ccgaactggc
     1381 ggaaccagcg caggcaggcg agcttgttga agtccatcgc ccacggcagg accatggtgt
     1441 tggcgacgcc gtgcggcacg tggaacatgc cgccgatggg atacgagagc gcgtgcacgg
     1501 cgccgacgcc ggcgttgccg aaggcgatgc cgcccatcat ggagcccatc gccatgttgt
     1561 cccgcgccca catgttggtg ccgtcgtaca cggccgtgcg gatgctctgg ctgatgagct
     1621 tgatgccctc gtaggcgagc gagtcggtgt gcatcgtggc gttgacggag atgtaggcct
     1681 cgatgttgtg gatgagggcg tccatgcccg aggcggcggt gacgttcggc gggcaggtga
     1741 gcgtgagcgc cgggtcgatg agcgcgacgt tcggcatgag gaacgggctg acgaggccct
     1801 gcttcacgtt gagcttctcg ttcgcgaaga tggcgatcgc ggtgcactcg gagccggtgc
     1861 cggaagtggt ggggatggcg atgatcggca ggcccttctt cttgacctgg tcgatgccca
     1921 cgtagtcgta cacggtgtcg cccgtgtgcg gcagcacggc ggcggccttg gcgacgtcga
     1981 tcggcgagcc gccgccgaag ccgacgatga ggtcgtagcc acccttctcg acgatccgga
     2041 cgatctcctc gatcgtgctc atgggcggct cgggcacggc cttgtcgtag aagtcgacct
     2101 cgaggccggc ctcggccaac ggccgctcga cgtcgatgca catgccgcac tcgctgacgc
     2161 cctggtcggt gatgacaagc accttgctgc cgttgagctt cttggcctcg acgccgagct
     2221 ggtcgaccgc gccgaccccg gtgatgagcg tcttgggctg gcgatggatg cgcatcttgc
     2281 gctcgacgcg gcgcagctcg gccggctggc cgctggcgga cgagatgacg cgcggttcgg
     2341 gttcgttgtg gacgtggtcc atggctctca gctctccttg gtgagcggtt ggcggggcag
     2401 gcggccgacc ccgtagacct cggccgcctc ctcccggatc tggtcgcgga acgccgggtc
     2461 ggcgatctcg atgagcgcct cggcgcgctc gcgcagcccg agcccgcgca ggtgcgcggc
     2521 cccgtgctcg gtgacgatgt agtcgacgtc ggcgcgcatc gtcgtgaccg cggtacccgg
     2581 cgcgagcgcg gccacgatgc gggagacgct gcccttcttc gccgtggacg gcaaggcgat
     2641 gatcgccttg ccgtcgcggc tgcgcgcggc gccgcgcacg aagtcgacct ggccgccgat
     2701 accggtgaac tgcgcggcgc ccatggagtc ggcgacgacc tggcccatca ggtcgacctg
     2761 gatggccgag ttgatggcga cgacccggtc gttctgcccg atgacgaacg ggtcgttgac
     2821 gatgttgacc ggcagcatgt tgacgaccgg gttgtcgtcg acgaagtcgt agaggcgccg
     2881 cgtgcccatg aggaacgtgg cgaccatctt gccggggtgg tacgccttgc gggcgttggt
     2941 gatcacgccc tcgttgtaga ggtcgacgac gccctcgctg aacatctcgc tgtggatgcc
     3001 gaggtcgttc ttctcgtgca ggaacagaag gacggcgtcg gggatgccgc cgatgccgag
     3061 ctggaggcag gagccgtcgt ccacgaggcc ggcgatgtgc ttgccgatgg cctcctcgac
     3121 ggggccgatc ctcggcggct gcagctcgat gatgtcctgg tcgctctcga cgaacgcgtc
     3181 gatgtccgag acgtggagga acgagtcgcc gtgggtgcgc ggcatcttgg gattgacctg
     3241 cgcgatcacc cgcccggcga cctgggcggc cggcttggtg tagtcgacgg agatgccgca
     3301 ggagcagaag ccgtgcttgt ccggcggaga gacctggatg aggaccacgt ccggcgtgag
     3361 gtacccctcg cagaagagct tggggatctc gctgaagaag acgggtacgt agtcgccgcg
     3421 gccgtccttg accgcctccc gggaggaggc gccgacgaac agcgacttgt ggtagaagct
     3481 gtcggcgtac tcgggccggc agtactcgcc cctgcccatc gcgaccatgt ggttgatctc
     3541 gacgcgctcg agctcgccgg accgggccac gagcgcgtcg accaggtcga gcggctcgcc
     3601 acaggcgtgc gcgaacacga cccggtcgcc ggacttgacg cacgccaggg cttcggcggc
     3661 cgtggtgcgc ttgccggcgt agtcggtctt ccagtccatc cccttcactc cttgagcttg
     3721 cgcgtcacgg cggggacgag ctcctccgac ggcgtgggca cgccgtcgac cagctcgccc
     3781 gccaccacga cgacgggtgg tccgtcaggg ccggcggcgg cgccctccgc gccggctccg
     3841 gcctgcccgt acagcggcgt agcgggctct tcaagcggtg ttccgtaggc gcaccgcttg
     3901 tcgccctccg cgacgagcat atggtccgag gcgcaggccc gggggcagcc gctgaggagc
     3961 acggtggtcc ccggacgaac gtccggatcg tctaccggca ggccggcagc gacggcggcg
     4021 cggtcgatgt gcggattgca gcccccgcag tacacgaccg ccacgcgtgc ctccgccggt
     4081 ctgccgtcgt cggtcatgtc actgagctac ggcggtcggc gccttgacct tggtcttgtc
     4141 ccacggcttg ccgacctcgg cgatcgcctt ggcgagctcc ttcttgtggt tcaggttgga
     4201 ctgccgcgag atcatgatgc gctgcgcctg cggcgacccg gcgccgtgca tggactcggt
     4261 gcggtagccg acggcgccgg cgccgatggt gaggttctcg atgaggcgca gcacgcgcat
     4321 gcggtcgatg gtcggcgtgt cgcactggcc ggcgaggaac ttctcgacga tgggcccgag
     4381 cttctcggag gcgaggtcct gggcggacgg catggtgacc atgaggccgc cggcgatgtc
     4441 ctcggccagg cgcgcgatct cgtaggggaa gcgcgtgacg ttctgcttgc agacgttggc
     4501 cagcagcatg tcgatctggt agttgccggc cggcatcttg acgccctcgt aggagcaggc
     4561 cagcccgcag ctgaacagcg tctcgttgag gtgcgtcatc tcgatgagct tgtccttgat
     4621 gtggctggcg cgggcggcgc cgttgtagtc ggcggccagg gcggccgcgc cgatgagcac
     4681 gtcgccgacg ccgaccttgc agccgccgta gctctggcga tggaagccgg cgaagcgctc
     4741 gacgagcgcg ccggagaact cgatctcgcc gcacatgtag acgcgctccc agggcacgaa
     4801 cacgtcgtcg aagaccatca gcgcttcctg gccgccgtag cgcatgttgc cgacgtccag
     4861 gtccgactcc tcgaacttgc gcgtgtcgca gctctggcgg ccgtagatgt agtagacgcc
     4921 ctcggcgttg gcggggcagc tgaagctgac cgcgtagtcc tcgtcgccct cgcgcatggc
     4981 gacggtgggc atgacgatga tctcgtgcga gttgcaggcg cccgtctggt gcgccttggc
     5041 gccgcgcacg acgatgccgt cgtcgcgctt ctcgacgatg tgcaggtaca cgtcggggtc
     5101 gggctgctgg tgcggcggca gcgagcggtc gcccttggga tcggtcatgg cgccgtcggc
     5161 gacgatgtcc tcgtcctgca ggtactgcac gtacttcgtg aagcgctggt ggtagtcggt
     5221 gcccctggcc tggtcgatct cgtaggtgac cgagtagagc gcgttgatgg cgtccatgcc
     5281 gacgcagcgc tggaagcacg agcccgtctt ctggccgagc aggcgctgca tcttgacctt
     5341 cttggagagg tcgtccgtgg actggtggat gtgggtgaac cggttgacct tcttgccggt
     5401 gaggtggctc gtggcgagca tcagatcctc gtactccggc tgcgccgcca tctcgtaggt
     5461 cgtggcgatg cagttgatgc tcggccggat gagggggtga ttgacgtagt cgtcgatctt
     5521 ctcgccgaag aggtagatct gcagatgcag gtcggcgagg ctctggacga actcctcggg
     5581 cgtcttgaga gccatgcggt gcactccttc ctggcgccct cgggtgcgcg ggcgcggcgc
     5641 tggcctatcc gggcgcgggc gcgcgcaggt gcgggagcgc tcacgcggtg agtcggtgcg
     5701 gcgggggcgg aagggtccgg ggggtcctgc gccctcgcgt ctcactatag gaggacgtgt
     5761 tctcgtagtc aatgaaggcg aacggagagc caatcagagt gacctttctt gtacggaatg
     5821 tacaaaacgg gatgtccgga agtggcgggt tcggaagcaa gtctcgccac tcgtggcaga
     5881 cgcggcagag gaaacccctg gccggatgga cgacctgtgc gccatggtgt accaagtggc
     5941 ccaccccggc gtcctaagtt tggcccgtat ggcgactgcc gcgtgtccgg ggaggtcgta
     6001 tactgcggtc gcctcgcggc ccgcgcgatc accatcgcgc gggcgcgtgc ggagcctgaa
     6061 cgcgcgatca tcgcacccaa ttgcctacgt ggagggcact acatgagcgc attcgttgac
     6121 gaattcatgg ccgaactcac ggccaagaac cccggcgagc cggagttcca ccaggccgta
     6181 ctcgaggtag ccgagtctct tgaggtcgtg ctcgacaaga acccgatcta ccgcaagatg
     6241 aaggtcctcg agcgcatcgc agagcctgag cgcgtgttcc tcttccgtat cccgtgggtc
     6301 gacgacagcg gcgagatcca gatcaacaag ggctaccgca tccagatgaa cagcgccatc
     6361 ggcccctaca agggcggcat gcggctccac cccagcgtca acctcggcgt cctcaagttc
     6421 ctcgctttcg agcaggtctt caagaactcg ctgacgaccc tccccatggg cggcggcaag
     6481 ggtggctcca acttcgatcc caagggcaag agcgacgccg aagtcatgcg cttctgccag
     6541 agcctcatga cggaactgtg caagtacatc ggccagttca ccgacgtgcc cgccggtgac
     6601 atcggcgtgg gcggccgcga gatcggcttc atgttcggtc agtacaagcg catccgtggt
     6661 gagttcgtgg gagtgctcac cggcaagggc cgcaactggg gcggctcgct catccgtccc
     6721 gaggcgacgg gctacggcgc cgtgtacttc gccgccgaga tgctcaccac ccgcggcgag
     6781 acgctggaag gcaagacctg cctcgtgagc ggctcgggca acgtcgccca gtacgcgacc
     6841 gagaagatcc tcgacctggg cggcaagccc gtcaccctca gcgactccgg cggctacatc
     6901 tacgacgagg ccggcatcga ccgcgagaag ctcgcgttcg tgctcgagct caagaacgtg
     6961 aagcgcggcc gcatcagcga gtacgccgac aagttcaaga gcgcggtcta cacgccggtc
     7021 gacccggcgc tcgaccacaa cccgctctgg ggccacaagg ccgagtgcgc cttcccgtgc
     7081 gccacgcaga acgagatcaa cgcgaaggac gccgcgaccc tggtggacaa cggcgtctac
     7141 gtcgtgtccg agggcgcgaa catgccgacg gtgcccgacg gcgtcaacct cttcgtcgag
     7201 aagaagctgc tctacggccc cggcaaggcc gccaacgccg gcggcgtggc cacctccggc
     7261 ctcgagatga gccagaacag tctgcgcctc tcgtggacgc gcgaggaagt cgacgctcgc
     7321 ctgcacggca tcatgaaggc catccacaag cagtgcaccg acgccgccga ggcgtacggc
     7381 atgaaggaca actacgtggc gggcgccaac atcgccggct tcctgaaggt cgccgacacg
     7441 atgctcgatc acggcgtcgt ctgagcccgg tacgggtagc tacgacgcga gcacaagctg
     7501 acgatcactg agcatccctc gccgcgccac cgcgcggcga gccgaaagga gcggcgagca
     7561 tgaagaagat cgagtgctac atccagccgt acaacctcga cgccgtggct gaggccctgg
     7621 cggtcgaggg cgtggtgggc atgtcggtcg ccgaggtcaa gggcttcggc gtccagaagg
     7681 ggttcgtccg cggcgaggac gtcaagccgg gggagtaccg cttccacccc aagatgaagg
     7741 tcgagatggt cgtgctcgag aaggacgtgg agcgcatcat caccgccatc aacgcctctc
     7801 tcaagagcaa ccagatcggc gagggcaaga tcttcgtgct cccgatcgac gacgccgtgc
     7861 gcacgcgcac cggcgagcgc gggcgcgacg cgatcacctg agtccgcgcg gccgcgcagg
     7921 ccgcgcgcga agacatgaag cacgaggggc ccggctgcgc cgggcccctc gtgcgacggg
     7981 ggcggccgtg aggccgcccc cttcttcgcc caccggcacg ggccgcgcgc ctctcccggc
     8041 ggcgctgccc gggcgctctt ccgtcaggcc agctcgaact ccttgaggaa gtctcccttg
     8101 gagcgtgcgc cgatgacctt cttggccggc tcgccgtcct tgaacaggat gatcgtgggg
     8161 atgctcatga tgccgtactt ctgcgccgtc gccgggttct cgtcgacgtt gagcttgccg
     8221 acgctcacct tgtcgccgtg ctcctgggcg atctcgtcga tgaccgggcc gaccatgcgg
     8281 cacgggccgc accacggggc ccagaagtcc acgatcagag gcgtgccggc gtccttcact
     8341 tcggtgtcga acgtcgcgtc ggtcagggtc ttcacatcag ccacgtgctc tctcctcctt
     8401 ggcgcggcag gtcctggccc tgcccgtttg tcgcgattgt gtcacagact gcccagcact
     8461 ctgtcgagac gctcctggac ctggaccgcg atgggctcca ggtccttgtt gcccaccatg
     8521 ccgagcatgg cgccggcgtt gacggcggtg acgcgtgttc cctggtcggt cgcgtagacg
     8581 acgacgttgc acgggagcag cacgccgagg tcgggctcca cctcgagcgc ggcgtgcgcc
     8641 agcttggggt tgcaggcgcc gaggatcgtg tgcggcggga cgtcgacgtc gagcttgtcc
     8701 ttcatcgtct gccggacgtc gatctcggtg atgacgccga atccttcggc cttgagcgcc
     8761 tccacgacgc gcgggagcgc cgactcgtac ggttcatcga gataccggcc tacgccgtac
     8821 tccacactct ccatggcgaa cctcctcgct ggacggggga ccgcgacgct tatacccagg
     8881 ggggtatttg actatgcctc ggcccctccg cgatgcacac gagcaagatg cccgtcctgc
     8941 gcggacgcca atccggtatc attccaggtt catggccgcc acccgcaggc gagcggctga
     9001 tccgccggtg acgaggagag actgccgtga gaagcgacga gatcaagcag ggcctggcgc
     9061 gcgcgcctca tcgctcgctg ttgcgcgccc tgggcgtgag cgacgccgag atggagaggc
     9121 cgttcgtcgg cgtcgtcaac gccttcagcg aggtcgtccc cgggcacctg cacctgcgca
     9181 gcgtcgtcga tgcggtcaag ggcggcgtgc gcgcgggcgg cggcgtgccc atggagttcg
     9241 gcgtcatggg catctgcgac gggctcgcga tgaaccaccg cggcatgctg tactcgctgc
     9301 ccagccgcga gctcatcgcc gacacggtcg agtccatgac catcgcccac gcgttcgacg
     9361 cgctcgtcct cgtgccgtgc tgcgacaaga tcgtcccggg gatgctcatg gccgcgatgc
     9421 ggctcaacgt gcccgcgatc atggtgggcg gcggcgccat gctggccggc catctcgagg
     9481 gccgcaccct cgatctcaac agcgtcttcg aggccgtcgg ccggcaccag gccggcgagc
     9541 tcgacgacga gagcctcaag gcgatcgagt gcaacgcctg cccgacctgc ggctcgtgtt
     9601 ccggcatgtt caccgccaac tcgatgaact gcctcgcgga ggtcatcggc atggcgctgc
     9661 cgggcaacgg caccatcccg gccgtgtaca gcgagcgcat ccgcttcgcc aaggacagcg
     9721 gcgagcaggt catgcgggtc ctcgccgccg accgcaagcc gcgggacatc ctcacggagg
     9781 cggcggtcgg caacgccctc gccgccgacg ccagcctcgg ctgcagcacc aacacggtgc
     9841 tccacctcgc ggccatcgcc accgaggcgg gcctggactt cgacctgcgg cgcatcaacg
     9901 aggtggcggc gaaggtgcct cacatctgca agatcgctcc ggccggcacg caccacatgg
     9961 aagacctgta ccacgccggc ggcatccagg cggtcatgaa ccgcctcatc ggcgccggcc
    10021 tcatggacgg ctcggcgctc agcgtggccg gcggcaccgt cggcgaggcc gtcgccaagg
    10081 cgcgcgtcct cgacgacgcc gtcatccggc cggtcgacga cccgtggcac gccaccggcg
    10141 ggctcgccgt gctcttcggc aacctcgcgc cggccggcgc cgtggtcaag gagggcgccg
    10201 tggccgacga gatgctcgtg cacagcggcc cggcgcgcgt cttcgagagc gaggccgacg
    10261 ccgtggcggc gatcgccgcg cgcgacatcg ccgacggctc cgtcgtcgtc atccgcagcg
    10321 aaggccccaa gggcgcgccc ggcatgcccg agatgctgag cgccacctcc atgctcgccg
    10381 gccagggccg ggacaaggac gtggcgctca tcaccgacgg ccgcttcagc ggcgccacgc
    10441 gcggcgcggc cgtcggccac gtgtcaccgg aggcgcacgc cggcggcccc atcgccgccg
    10501 tgcgcgacgg cgacaccatc agcatcgaca tccctgcccg caccatcacg ctcgagctcc
    10561 ccgaggagga gatcgcgcgg cggctcgccg actggcggcc tgcgccgcgc gacggcgtaa
    10621 ccggctatct cgcccgttac gtcaaactcg tctccggcgc ggagaaggga gctgtgctgc
    10681 aatgacacca gacagacgcg tgaagggctc gcaagccctt gtcgccgccc tcgaacacga
    10741 gggctgcaag gtcctgttcg ggtaccccgg cggggtggcg atcccgttct acgacgcgct
    10801 ctacgacgcc aaggagctgc gccacgtgct cgtgcgccac gagcagggcg ccgcgcacgc
    10861 ggccgacggc tacgcccgct cgaccggcaa ggtgggcgtc tgcacggcca ccagcggtcc
    10921 cggcgccacc aatctcgtca ccggcatcgc cacggcgtac ctggactcgt cgccgctggt
    10981 cgccatcacc tggcaggtgc gcagcgacct catcgggacc gacgccttcc aggaggccga
    11041 catcaccggc atcacgctgc cgatcgtcaa gcactcctat ctggtcaagg acccggcgga
    11101 gctgccgcag accgtccacg aggcgttcca catcgcctcc accggcaggc ccggccccgt
    11161 cgtcatcgac atccccgtcg acatcgcgct gggcgagctc gcctaccgtg acgccgacac
    11221 cctcgacctg cccggctaca agcccacgtt caagggccac atcaagcaga tccgcgccgc
    11281 cgccagggcc atcaacgagg cccagcggcc ggtgctgtac ctgggcgggg gcgccatcac
    11341 ctccgacgcg gcgcccgaga tcctcaagct cgcggagctc cgccgcattc cggtggcgac
    11401 cacgctcatg gcgctcggcg ccttccccga gacgcatgat ctgtccatg
//
LOCUS       DASUMX010000089         6724 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1749275, whole genome shotgun sequence.
ACCESSION   DASUMX010000089 DASUMX010000000
VERSION     DASUMX010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 6724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6724
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1749275"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1008)
                     /locus_tag="VFZ86_04310"
     CDS             complement(<1..1008)
                     /locus_tag="VFZ86_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641548.1"
                     /translation="MTGRGSRVTRALTSRRAPRLRTGVAAFLVAASVLAVLVSALALW
                     THGLVFDTDTYVGVVVPVLDDPDARKALGEYAASTAVRAVDLDARIEAALPSDAAVLA
                     PALTSSLQAFLAGEIDRFLGTEPARRLWAEGNRLAHRQLVTTLQDENGAVTAGEDDVR
                     LDLLPLVAVALQRLEARVPGILGRDVTLPEIEPDRAPGEIRTLLQDALGRGLPADLGS
                     VTLLRGTQAREAKRTLSLFNDLVILVVALTVALIVAALLVSVRRLRTAFWLGLGSLLA
                     FVVARVIAARLEQAVVDAVETGGGAAVARSVLSSAVRSLDAFFVWIAVAGAIVAVAAL
                     LA"
     gene            complement(1005..2174)
                     /locus_tag="VFZ86_04315"
     CDS             complement(1005..2174)
                     /locus_tag="VFZ86_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX5641549.1"
                     /translation="MSQPPVFTQNALREGFVEFAFGEPDPALLPVGLVREAAARALGA
                     EGAAALAYGSSAGPAALRAAIAQRTAAREGLLLAESDVLVSGGISQAVDQVLTVLAAP
                     GDVVFVECPTYNLALGIIADHPVDVVGVPLDAEGIDVDALAAAVREARASGRRPRLLY
                     TIPTFHNPAGVSLSAPRRKQLLELARREDLLLVEDDVYRELVYEGEAPPALRALDPAA
                     PVIRLGSFSKSLAPGLRLGWIDAPAALRERLAAYGTLESGGCVSQFSAHVVAALLAAG
                     AYTAHVAALRHAYASRRDALAAALRDHLPAGCAFTVPAGGFFLWVTLPPGLAASRLLP
                     VAERHRVAFAPGGRFCADGDDRSLRLAFSLYDEASLAEGARRLGAAVTEAAGGAA"
     gene            2284..3837
                     /locus_tag="VFZ86_04320"
     CDS             2284..3837
                     /locus_tag="VFZ86_04320"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013421862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HEX5641550.1"
                     /translation="MLFPTITFAVFFLLVFVGNWLLMPRQRVWKPFMLVVSLVFYGWW
                     DWRFVLLLALSAVANQFFALWIAGLPEHGPRRRWALAAAVAANLGVLGWFKYYGFFVT
                     SAADALEALHLNADLPLLRIVLPLGISFLTFRVLSYVIDVERGTLRPASLLDFSVYVA
                     FFPYLAAGPIARASEFLPQLRGPRDPRKVDTARAFFLIFGGLVKKMLIADYLATHIVN
                     GVFTTPGQYSSLEVLVGIIGYSVQIYCDFSAYADIAIGISLLLGFELPDNFDAPYTAR
                     SVQEFWRRWHMTLSRWLRDYLYIPLGGNRRGRARTYVNIMLTMLLGGLWHGAGWTFVF
                     WGFLYGAALVVEHLRLDGRKERALLVRRQRLDLAAASEGLAAAPAAAGAPAPGGATAG
                     SPPGAPLEYDRQPWPGVWGPRIGVFAFVTFAWIFFRSDSFAAALDVIARLFQSWGTIG
                     AAVTPGVFLAIAVGIGVQYVPRSLWQRGEARLSALNPVLQGVALAVAFMLLDILGPTG
                     PAAFLYFKF"
     gene            3837..4976
                     /locus_tag="VFZ86_04325"
     CDS             3837..4976
                     /locus_tag="VFZ86_04325"
                     /inference="COORDINATES: protein motif:HMM:NF016218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF459 domain-containing protein"
                     /protein_id="HEX5641551.1"
                     /translation="MAGDQTPRDERSGGTDPGDERRRSGLPFGRRRRTAASALVAMLV
                     ALALGALLNAPAMQKTALALPFGAERSFRLALVDPLATVSHWLFLDRPAKLTAVALGK
                     PDPGPTGTSVVVVRPTPGPTAGGKPGDDGDKPRKTLQERALPKPYKGHPMHLYIAGDS
                     MLGLPGMALTNLSIKTRLIKPLLDYRISTGLVRPDAFNWPAQLQRQVRAFRPGAVAVM
                     FGANDNQPLQAASGEIHQFGSDGWKREYRRRVQDVVGLLFQNGVRRVYWIGQPVMPEA
                     SFNGQIRLMNEIYRGVAEQTFGVRYIDAYSLLADGRGEYAQYLPDEHGELQQVREQDG
                     EHLTYAGGLRIAGAVLDEIGREWLPKKGEGDEPPPGPGASATPAP"
     gene            5118..6557
                     /locus_tag="VFZ86_04330"
     CDS             5118..6557
                     /locus_tag="VFZ86_04330"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009741986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HEX5641552.1"
                     /translation="MLFPTITFAIFFLVVYVLNWLLMPRLRLWKWFIIAAGYVFYGWW
                     DWRLVFLLLGATLVNQALGVQVHRALGRRGSRAAARPWVVLAVAVNLVTLGVFKYFDF
                     FVVNLNDVLGVVGLGAPLPLLRIILPVGISFLVFRMLTYVIDIYRGQLAPAPALDFAV
                     YTAFFPYLLAGPIARAREFLPQLTSPRDPRTVDSSRAFVLILGGLVKKLLIADYLSTH
                     IVNGLFASPQTYSSWETLWGIVAYSVQIYCDFSAYADIAIGASLLLGFKLPANFDAPY
                     TARTIQDFWRRWHITLSSWLRDYLYIPLGGNRKGRGRTYVNLVLTFLLGGLWHGAGWT
                     FVFWGLLHGAGLAFERARMDVRHTAGLPEPGATAGARALQRLGVFAFVSVAWVFFRAE
                     SMGNAFTVLWRLIAGIGSLGTAVTLPVVGLAVLGIAIQYVPRRTIEGLEAGFSRIGWV
                     GQGLVLAAALFLIDVLGPAGPAEFLYFKF"
BASE COUNT          854 a         2486 c         2409 g          975 t
ORIGIN      
        1 ggcgaggagg gcggccaccg cgacgatcgc cccggcgacc gcgatccaga cgaagaacgc
       61 gtcgaggctc cggaccgcgg agctcaaaac ggagcgcgcc accgctgccc cgcccccggt
      121 ctccaccgcg tccacgacgg cctgctccag ccgcgccgcg atcacgcgtg ccacgacgaa
      181 ggcaagcagc gacccgaggc cgagccagaa cgccgtccgc aggcgccgca cggagacgag
      241 cagcgccgcc acgatgagcg ctacggtgag cgcgaccacg aggatcacga ggtcgttgaa
      301 caggctgagc gtccgcttcg cctcgcgcgc ctgggtcccc ctgagcagcg tgacggagcc
      361 gaggtccgcc ggcagtccgc gaccgagggc gtcctgcagc agcgtcctga tctcgccggg
      421 cgcgcggtcg ggctcgatct ccgggagggt gacgtcgcgc ccgaggatcc cggggacccg
      481 tgcctccagt ctctgcaggg ccacggcgac gagcggcagg aggtcgagcc gcacgtcgtc
      541 ctcacccgcc gtcacggctc cgttctcgtc ctgcagcgtc gtgacgagct gccggtgggc
      601 gaggcggttg ccttccgccc agaggcgccg ggcgggctcg gtgccgagga accggtcgat
      661 ctcgccggcg aggaaggcct gcagcgagct ggtgagcgcg ggcgcgagca ccgcggcgtc
      721 ggagggcagc gcggcctcga tgcgcgcgtc caggtccacg gcccggacgg ccgtggaggc
      781 cgcgtactcg ccgagcgcct tccgggcatc cgggtcatcg agcacgggga cgaccacgcc
      841 gacgtaggtg tcggtgtcga agacgaggcc gtgcgtccac agggcgaggg cgctcaccag
      901 cacggcgagc accgaggccg ccacgaggaa ggcggcgacg cccgtgcgga ggcggggcgc
      961 gcgccgggaa gtcagggcgc gcgtcactcg cgagccgcga ccggtcacgc agcgccgcct
     1021 gcagcctcgg tcacggcggc gccgagccgg cgggcgccct cggcgaggct cgcctcgtcg
     1081 tagaggctga aggcgagccg caggctgcgg tcgtcgccgt cggcgcagaa ccgcccgccc
     1141 ggagcgaacg cgacgcggtg gcgctcggcc accggcagca ggcgtgacgc ggccaggccc
     1201 ggcggcagcg tgacccagag gaagaagccg ccggcgggca cggtgaaggc gcatcccgcc
     1261 ggcaggtggt cgcgcagcgc ggcggccagc gcgtcgcggc gggacgcgta ggcgtggcgc
     1321 agcgcggcga cgtgggcggt gtaggcgccc gccgccagca gcgccgccac gacgtgggcg
     1381 gagaactggc tcacgcagcc gccactctcg agcgtgccgt aggccgccag ccgctcgcgc
     1441 agggcggccg gggcgtcgat ccagcccagc ctgaggcccg gcgccagcga cttggagaac
     1501 gagccgagcc ggatgacggg cgccgccggg tccagggcgc ggagcgccgg cggcgcctcg
     1561 ccttcgtaca cgagctcgcg gtagacgtcg tcctcgacga gcaggagatc ctcgcgccgg
     1621 gcgagctcga gcagctgctt gcgccgaggc gccgagaggc tgacgcccgc agggttgtgg
     1681 aaggtcggga tcgtgtagag gagccggggg cggcggcctg acgcgcgcgc ctcgcgcacc
     1741 gccgcggcca gcgcatccac gtcgatcccc tctgcgtcca ggggcacgcc gaccacgtcg
     1801 acggggtggt cggcgatgat ccccagggcc aggttgtagg tggggcactc gacgaagacc
     1861 acgtcgccgg gcgcggcgag cacggtcagc acctggtcga cggcctgtga gatcccgccg
     1921 ctgacgagca cgtcgctctc ggccaggagg aggccttcgc gcgcggcggt gcgctgcgcg
     1981 atcgccgcgc gcagcgccgc cgggccggcg ctggatccgt aggcgagcgc cgctgcgccc
     2041 tcggcgccga gcgccctcgc ggcggcctcc cggacgagcc cgaccggcag cagcgccggg
     2101 tcgggctccc cgaaggcgaa ctcgacgaag ccctcgcgca gggcgttctg ggtgaagacg
     2161 ggaggctggc tcacggcgtc atgatacgcg atgcgcctcg cgccggcgcg gcatgcggtg
     2221 cctgcgaacc ccggctggct acccgccgtc cctttcccta gactggggga ccagaaccgc
     2281 cgcatgctgt tcccgaccat cacattcgcc gtcttcttcc tgctcgtctt cgtcgggaac
     2341 tggctgctca tgcccaggca gcgcgtgtgg aagccgttca tgctcgtcgt cagcctggtg
     2401 ttctacgggt ggtgggactg gcgcttcgtc cttctcctcg cgctcagcgc cgtggccaac
     2461 cagttcttcg cgctctggat cgcggggctg ccggagcacg gcccgcggcg caggtgggcg
     2521 ctggccgcgg ccgtcgccgc caacctcggc gtgctcggct ggttcaagta ctacggcttc
     2581 ttcgtcacct ccgccgccga cgccctcgag gcgctccacc tgaacgccga cctgccgctg
     2641 ctgcgcatcg tcctgccgct cggcatctcg ttcctcacgt tccgcgtgct gagctacgtc
     2701 atcgacgtgg agcgcggcac cctgcggccg gcgtcgctgc tcgacttctc cgtgtacgtc
     2761 gccttcttcc cgtacctcgc cgccggtccc atcgcccgtg cctcggagtt cctgccgcag
     2821 ctgcgcggcc cgcgcgatcc acgcaaggtg gacacggcgc gggccttctt cctcatcttc
     2881 ggcggcctgg tcaagaagat gctgatcgcc gactacctcg ccacccacat cgtcaacggg
     2941 gtgttcacca cgccgggcca gtactcgtcg ctcgaggtgc tggtcggcat catcggctac
     3001 tcggtgcaga tctactgcga cttcagcgcc tacgccgaca tcgccatcgg catctcactg
     3061 ctgctcggct tcgagctgcc ggacaacttc gacgccccct acacggcgcg ctcggtgcag
     3121 gagttctggc gccgctggca catgacgctc tcgcgctggc tgcgcgacta cctgtacatc
     3181 ccgctgggcg gcaaccgcag gggccgcgcg cgcacgtacg tcaacatcat gctgacgatg
     3241 ctgctcggcg gcctctggca cggcgccggg tggacgttcg tcttctgggg cttcctgtac
     3301 ggcgccgcgc tggtcgtcga gcacctgcgg ctggacggcc gcaaggagcg ggcgctgctc
     3361 gtccggcgcc agcggctcga cctggcggcg gcgtccgaag gcctcgccgc cgcgccggcc
     3421 gccgcagggg cgccggcgcc cgggggcgcg acggcgggct cgccgccggg cgcgccgctc
     3481 gagtacgacc ggcagccgtg gcccggcgtg tggggaccgc gcatcggcgt cttcgccttc
     3541 gtcaccttcg cctggatctt cttccgctcc gacagcttcg cggcggcgct ggacgtcatc
     3601 gcccggctct tccagagctg ggggacgatc ggcgccgccg tgacgccggg cgtgttcctg
     3661 gccatcgccg tcgggatcgg cgtgcagtac gtgccgcgct cgctctggca gcgcggtgag
     3721 gccaggctct ccgcgctcaa cccggtgctc caaggggtcg ccctggcggt cgccttcatg
     3781 ctcctcgaca tcctcgggcc cacgggaccc gcggccttcc tctacttcaa gttctgatgg
     3841 ccggcgacca gacacctcgc gacgagcgca gcggcgggac cgatcccggg gatgagcgcc
     3901 gccgctccgg gctgccgttc ggccgccgcc gccgcaccgc cgccagcgcc ctggtggcga
     3961 tgctcgtggc gctggcgctc ggcgcgctgc tcaacgcgcc ggcgatgcag aagaccgcgc
     4021 tcgcgctgcc cttcggcgcc gagcgcagct tccgcctcgc cctcgtcgac cccctggcga
     4081 cggtgagcca ctggctgttc ctcgaccgcc cggcgaagct cacggcggtc gcgctcggca
     4141 agcccgaccc cggccccacc ggcacctcgg tcgtggtggt caggcccact cccggtccga
     4201 ccgcgggggg caagccgggg gacgacggcg acaagccacg caagacgctg caggagcgcg
     4261 cgctgcccaa gccgtacaag ggccacccga tgcacctgta catcgccggc gactcgatgc
     4321 tgggcctgcc cggcatggcg ctgaccaacc tctccatcaa gacccggctg atcaagccgc
     4381 tgctggatta ccgcatctcc accggcctgg tgcgcccgga cgccttcaac tggccggcgc
     4441 agctccagcg gcaggtcagg gcgttccgcc cgggcgccgt ggcggtgatg ttcggcgcca
     4501 acgacaacca gccgctccag gccgcctccg gcgagatcca ccagttcggc tccgacggct
     4561 ggaagcggga gtaccgcagg cgcgtccagg acgtggtcgg cctgctgttc cagaacggtg
     4621 tgcggcgcgt gtactggatc ggccagccgg tcatgcctga ggcctccttc aacggccaga
     4681 tcaggctgat gaacgagatc taccgcggcg tcgccgagca gaccttcggg gtgcgctaca
     4741 tcgacgcgta cagcctgctc gccgacggcc gcggagagta cgcgcagtac ctgcccgacg
     4801 agcacggcga gctgcagcag gtgcgcgagc aggacggcga gcacctcacg tacgccggcg
     4861 gcctgcgcat cgccggcgcc gtgctcgacg agatcgggag ggagtggctc ccgaagaagg
     4921 gcgagggaga cgagccgccg cccgggccgg gcgcgtccgc gacgccggct ccctgacgcc
     4981 gggcgcgtcc gcaacgccgg ctcaccggcg cggcgcgcgg gtgcttcgcc gcccgcgacc
     5041 tctggtatag catccgcaac cagcaggcgg ggccgccggc gcggccacgc gcacccgggt
     5101 gatcgacagg cggcggcgtg ctcttcccga ccatcacgtt cgcgatcttc ttcctcgtcg
     5161 tctacgtgct caactggctg ctgatgccgc ggctgcggct gtggaagtgg ttcatcatcg
     5221 ccgccggcta cgtgttctac gggtggtggg actggcggct ggtcttcctg ctcctcgggg
     5281 cgacgctggt gaaccaggcg ctcggcgtgc aggtgcaccg cgcgctcggg cggcgcggct
     5341 cgcgggccgc cgcccgcccc tgggtcgtcc tcgccgtcgc cgtcaatctg gtcacgctcg
     5401 gcgtcttcaa gtacttcgac ttcttcgtcg tgaacctcaa cgacgtgctc ggcgtcgtcg
     5461 gcctgggcgc gccgctgccg ctgttgcgca tcatcctgcc ggtcggcatc tcgttcctcg
     5521 tcttccgcat gctgacctac gtgatcgaca tctaccgcgg ccagctcgcg ccggcgccgg
     5581 cgctggactt cgccgtgtac acggccttct tcccgtacct cctcgcgggc cccatcgccc
     5641 gcgcccgcga gttcctgccg cagctcacct cgccgaggga cccgcgcacg gtggactcct
     5701 cgcgcgcctt cgtgctcatc ctcgggggtc tggtcaagaa gctgctcatc gccgactacc
     5761 tctcgacgca catcgtcaac gggctgttcg cgtcgccgca gacgtactcg tcgtgggaga
     5821 ccctctgggg catcgtcgcc tactcggtgc agatctactg cgacttcagc gcctacgccg
     5881 acatcgccat cggcgcctcg ctgctgctcg ggttcaagct gcctgccaac ttcgacgcgc
     5941 cgtacacggc gcgcaccatc caggacttct ggcgccgctg gcacatcacc ctctcgagct
     6001 ggctgcgcga ctacctgtac ataccgctgg ggggtaaccg caagggccgc ggccgcacct
     6061 acgtcaacct ggtgctcacg ttcctcctcg gcggactctg gcacggcgcg ggctggacgt
     6121 tcgtgttctg ggggctcctg cacggcgccg ggctcgcgtt cgaacgcgcg cggatggacg
     6181 tgcgtcacac ggcgggcctg cccgagccgg gcgcgaccgc cggcgcgagg gcgctccagc
     6241 gtctcggggt gttcgcgttc gtcagcgtgg cctgggtgtt cttccgcgcc gagtcgatgg
     6301 gcaacgcgtt cacggtgctc tggcggctca tcgccgggat cgggtcgctg ggcacggcgg
     6361 tcacgctccc ggtggtgggc ctggcggtgc tcggcatcgc catccagtac gtgccgaggc
     6421 ggacgatcga gggtctggag gccggcttca gccgcatcgg ctgggtgggc cagggtctgg
     6481 tgctggcggc ggccctgttc ctgatcgacg tgctggggcc ggccgggccg gccgagttcc
     6541 tgtacttcaa gttctgagcg accgagggcg acgggcggac gagacgatga ccgacgacag
     6601 gccatacgag gacgagcgcc cggaccggcg cccgcccggg taccccacgg ggccgggccg
     6661 gccacgtcgc gaggggccgc ggcggcgccg ccgctcggcc ggcgcggtgc tcctcgccat
     6721 gctg
//
LOCUS       DASUMX010000090        13406 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_24248, whole genome shotgun sequence.
ACCESSION   DASUMX010000090 DASUMX010000000
VERSION     DASUMX010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 13406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13406
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24248"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="VFZ86_04335"
     CDS             <1..358
                     /locus_tag="VFZ86_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX5641553.1"
                     /translation="GVPLWASVLAYGTGAYAAAVDHCVVTAEYAAERIAGTSGLELVI
                     EPAFTVLLVRRPGWAPDDYAAWCGDALARGVAMLMPTRHAGETVLRFCFVNPLTTRED
                     VDVVLSDLAASSPSPP"
     gene            complement(321..1760)
                     /locus_tag="VFZ86_04340"
     CDS             complement(321..1760)
                     /locus_tag="VFZ86_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX5641554.1"
                     /translation="MTDGSRFYIDGQWVEPAHPGDEIVVVNPATEEVAARIAAASPAD
                     VDAAVQAAHRAFAGYSQTTREERIELLTRLREAYKRRIPAIAAAMTTEVGIPLGLAQG
                     GQVLAGARHLKEAMEALRDYPLEQDWGSTRIVREPVGVCALLAPWNWPMNQVTAKVGP
                     ALAAGCTMVLKPSQLSPLSALLFAEAADEAGLPPGVFNMVNGAGAALGDAFATHPLVD
                     MVSITGSTAVGGLVAAAAAPTIKRVSQELGGKSANIVFGDVNVERVVRSGTLGCMHNS
                     GQTCNAPTRMLVAEEIYEEAVAVAARAADGVAVGDPLDQATFMGPVAGRKQFDTVRNY
                     IDVGAAEGARLVAGGPERPEGLDRGFFVRPTVFADVAPDMRIFREEIFGPVLCITPFR
                     DEEHAIELANATDFGLSGYVWSADLERARRVAGRMRTGMVHVNGAPTDSRAPFGGYKQ
                     SGNGREWGRFGLEDFLEVKAVMGWKPPGR"
     gene            1932..2765
                     /locus_tag="VFZ86_04345"
     CDS             1932..2765
                     /locus_tag="VFZ86_04345"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX5641555.1"
                     /translation="MLAALLALAASFTWGTSNYLAGVETRTRSVWHVAALSQLAAAAA
                     AAFGLVAATQPPPGGWDLFLLVVTGIVTAAGLVMFYKALAIGTMSVVAPIIAAQVLIP
                     VAAGILLGERPGPHAYVGMVVTVAGVLLVSWARRGTRDRVARKAVVLAALTAVAWGFM
                     LLIYGTTGKENPVWTVFDTRVASAVVLAGYVLLTGRGLSLKGQNLLVLAAIGILLAAA
                     NFLFIVATGLGYLSVTSILGSLSPVVVTAYAQVLLHERLAPRQWAGFVAVFAGIVLLS
                     V"
     gene            2913..3134
                     /locus_tag="VFZ86_04350"
     CDS             2913..3134
                     /locus_tag="VFZ86_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018280994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEX5641556.1"
                     /translation="MQLKLTAVFEQVPEGYIGYVEELPGANTQGATLDETRENLVEAV
                     ELVLDANRLLAEERLGDRDVIREPLRISA"
     gene            3131..3316
                     /locus_tag="VFZ86_04355"
     CDS             3131..3316
                     /locus_tag="VFZ86_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016514546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEX5641557.1"
                     /translation="MKRADLVRHLESHGCELLREGGRHSVYANRAAGKATAVPRHREI
                     NDFLARKICRDIEVVEP"
     gene            complement(3362..5317)
                     /gene="typA"
                     /locus_tag="VFZ86_04360"
     CDS             complement(3362..5317)
                     /gene="typA"
                     /locus_tag="VFZ86_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009163600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEX5641558.1"
                     /translation="MHAASQIRNLAIIAHIDHGKTTLIDSIFRAAHVFRENAHVDERV
                     MDSNDLERERGITIRSKHCTVEWKDYLINIIDTPGHADFSGEVERVLSMVDSVLLLVD
                     ANEGPMPQTRYVLMRALSFGLRPIVIVNKADRPNADPSGALDKTFDLFVELGANNEQL
                     DFPVLYGSGLEGWIVNDIDEYKKEEHEREEHLHDGGGLSPALLRELADAGMNDLFETI
                     IESVPAPKVDQDAPFLMQVSTLAWSDYIGRIGCGRVLAGGLKVGDELQRITTRHLEIG
                     DPEAGWEMTGTEAARVTHLWVTRGLENREVEEASAGDIVWLAGPADITLGDTLASPEL
                     KAAAAALPPLEIEEPTVSMFFLVNNGPFAGQDGKAATLRQIKDRLERELRVNVALRVE
                     DLGRPDGVKVSGRGELHLAILIEEMRREGLELCVSRPEVITHRDGHGKLLEPMEQLII
                     DVPEEYQGAIIQELSLRKGELVGVESTGTGLVRLEFTISTRGLIGYRNDFLTETRGLG
                     VMSSRFTGYGPWKGELPGRERGSLVSQTAGEAAGYSLENLQQRATLFVGNMTPVYEGM
                     IVGENSRSDDMLCNPTKRKQLGNYRSSTKDIDAGLKVPRPITLESALEWIADDELVEV
                     TPKTVRVRKHILSTEQRKKFEKRGAEA"
     gene            5581..6747
                     /locus_tag="VFZ86_04365"
     CDS             5581..6747
                     /locus_tag="VFZ86_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020681187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HEX5641559.1"
                     /translation="MGEEPTIRIAQSCAAEPRVAVRELYAGLAQPELSLVLFFCSSDY
                     DRGALAEELRTRFGRTLVVGCTTAGEIGPLGCRDRSLTGVSFAPSAGTAVMGHLDDLR
                     GFSIPEGVEFARSLRRRLEDEAQQADAHNTFALLLVDGLFGHEEQLAHALQEGLGEIL
                     LVGGSAGDSMSFANTYTYRDGDFVSGSAVLVLITTPLPFIEFKTQHFEPTDERFVVTE
                     ADPDLRVVSEIDGLPAAEEYARMLGVDPRDLGPAHFAASPVVVLIDGGNYVRSIQKVN
                     PDGSLKFYCAIERGVVLRGAKGVDLVRNLEAALAQVRARIGPPQVVLAFDCILRRLEI
                     LQTGVEQPVADLLRANNAVGFNTYGEQYCGLHMNQTLTAVAIGSRRAAGGARNG"
     gene            6740..8233
                     /locus_tag="VFZ86_04370"
     CDS             6740..8233
                     /locus_tag="VFZ86_04370"
                     /inference="COORDINATES: protein motif:HMM:NF037653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5641560.1"
                     /translation="MADAPDTRDVATLQAEVARLNKIVDALMDRSEAGMDVKESDFGL
                     FQTTVRLQDQVRRHTEELEAALREHEAGPGEADASAARDMQTLRRTAALQIQLLELVV
                     QEKDVGELVDRVASILEVPVVLFDTQGQVVCASGRDLKDDGLVRGLWEAYERLRGLPG
                     PHAVVDREDDRVYFREVFIMDRVERVLAAVVSRRQPSDFAAASLLYLQQLVTLELLRK
                     RDELKMRRRVRRGLLRDILAGRSPTRDLRIRLEEQGFSATSVLRLAVVEPAALRPSSA
                     GTLAGKTADKSTGGVLRALDARLSQRRIPFLSLSNGPSVVVLTALPDAGTGPARDLLD
                     DLRAAATAATAAPERLVAGCSAPLAGVESAPRCLQQARAACMAARRSSEPVKGAVFDE
                     LSGHLILLDGLDQKALSDIVQRTFGPLLEYDAAHRTSLYKTLYTLFENHLAVQQTADE
                     LHIHRNTLQKRTAHVEELLGIDLSELDDIVDVRLGLQAAVLLGQQPS"
     gene            8387..8869
                     /locus_tag="VFZ86_04375"
     CDS             8387..8869
                     /locus_tag="VFZ86_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX5641561.1"
                     /translation="MAKVLMIHPDKCTGCRNCELACSFEHERRFRPSASRVQAFTWER
                     EGISVPMMCQQCDDAACVTVCPTGAMHKSSTTQAMIDWDDDKCIRCRMCVIACPFGNA
                     RYDAMTSSIFKCDNCDGAPQCAAFCPTAALEYVDDTAQVKARKKAYAAKFKDAFQEVS
                     "
     gene            8871..10679
                     /locus_tag="VFZ86_04380"
     CDS             8871..10679
                     /locus_tag="VFZ86_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HEX5641562.1"
                     /translation="MYGWTGTLLRVDLGSGKVTREALDPRVAREYIGARGLGGYIIRS
                     EVDPKVDALSPANKLVFAAGPLTGTFAPSAGRYNVCTKSPLNESLAASNSGGAFGPEL
                     KYAGFDAVIVEGKAAKPVYLWINDAEVEIRDAAGVWGMNVPDATDAIRAATDDEAKVA
                     CIGPAGEHLSLIANIMNEMHRAAGRTGVGAVMGSKNLKAVAVVGNGPVEVADPDAFLE
                     EVLKGRKMIFDDPVGGTGLPAYGTEVLVNILNTVGGLPTRNWQDGHFANADKTGGEAL
                     AAKQLVRKKGCFSCIISCGRVTKVDAPGFEGIGEGPEYETAWAYGADCGVDNLDAITK
                     ANYLCNEYGMDTIAAGSSIACAMELYQRGIITSKDTDGVDLTWGNAEAIVEMTRKMGE
                     GEGFGAKAAQGSYRLADGYGHPEYSMTTKKQEMPAYDGRAIQGIGLNYATSNRGGCHV
                     RGYTISHEVLGSGGNADPHVTEGKAGLGIAFQNLTAALDATGSCLFATFGLTGEQLVK
                     TLATLTGYDYTLEEFLQVGDRIWTQERLWNIAAGYTSADDTLPRRLLEEPLKTGASKG
                     HVNKLGEMLPEYYEQRGYDEHGVPTKAKLKELSLKA"
     gene            10676..10948
                     /locus_tag="VFZ86_04385"
     CDS             10676..10948
                     /locus_tag="VFZ86_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like small modifier protein 1"
                     /protein_id="HEX5641563.1"
                     /translation="MTVKYFATIRDLTRETERRVDGRPGDLRELLTELAERYGRPFRR
                     AVLDGDDLHGEIIVFVNGRNVRHLQMLDTPLADEDEIAIFPMIAGG"
     gene            11038..11529
                     /locus_tag="VFZ86_04390"
     CDS             11038..11529
                     /locus_tag="VFZ86_04390"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEX5641564.1"
                     /translation="MSDTRTIDTSRVTAVLRRYPRHDPGELVNVLHDLQAEFRYLPEE
                     ALRAAAAHLGLSRTQVYGVASFYHGFHTEPRGEHTCTVCVGTACHVRGAPRLLEQLER
                     DTGIAAGGTTPDLSLSIEEVGCVGACAIGPLVLLDGEHHGHMTPARLSRMVAKTVAGK
                     DEP"
     gene            11526..13370
                     /locus_tag="VFZ86_04395"
     CDS             11526..13370
                     /locus_tag="VFZ86_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEX5641565.1"
                     /translation="MSARLADPAALEDRREALGAGRDARRRVLAVCAGTGCRAQGALG
                     VVDALRAEVEARGVAGEVEVRATGCLGFCQASPLVLLVPDGVAYRHVEPADAADLVAA
                     TAAGELVHRLLCIDADGSCTLQDEIPFYRAQQRLLLGASALLDPTSIDAYITLGGYAA
                     LAKALDMGPEAVLEEVKASGLRGRGGAGFPTGRKWEACRRAEGELKYVVCNADEGDPG
                     AFMDDALLEGNPHGVLEGMVIGAYAIGAREGHVYVRQEYPVAVERITRAIEQARALGL
                     LGERILGSDFSFELRVSRGGGAFVCGEASAMMASLEGRRGVPRRRLIHLSERGLWGRP
                     TNLNNVETWANVPQIVLNGADWYRAIGTAGSPGTKIFSLVGKVARTGLVEVPMGTTLR
                     EIVFGAGGGVPNGKQFKAVQTGGPSGGCLPADKLDLPVDFDTLAAEGSMMGSGGMIVM
                     DEGTCMVDVARYFLRFLASESCGNCTSCRDGLALLRDLLGDVSEGRGTRETITLIEQL
                     ADAVEATSLCALGTSGVNPVLSTLRHFRDEYLAHVDEKRCPAGVCKALISFSILEERC
                     TGCRACALGCPQGCISGEKKQLHVIDAEDCIKCGICRDVCRFDAVLVQ"
BASE COUNT         1969 a         4764 c         4716 g         1957 t
ORIGIN      
        1 cggcgtgccg ctctgggcgt cggtcctggc ctacggcacc ggcgcctacg ccgccgccgt
       61 ggaccactgc gtggtcacgg ccgagtacgc cgcggagcgg atcgccggga cgtccgggct
      121 cgagctcgtg atcgagccgg cgttcacggt gctccttgtg cgccggcccg gctgggcgcc
      181 ggacgactac gccgcgtggt gcggggatgc gctggcgcgc ggcgtcgcca tgctcatgcc
      241 gaccaggcac gccggcgaga ccgtcctgcg cttctgcttc gtcaatccac tgacgacccg
      301 cgaggacgtg gacgtcgtgc tcagcgacct ggcggcttcc agcccatcac cgccttgacc
      361 tcgaggaagt cctcgagccc aaagcggccc cactcgcgtc cgttgccgga ctgcttgtag
      421 ccgccgaagg gagcgcggct gtcggtcggc gcgccgttga cgtgcaccat gccggtgcgc
      481 atgcggccgg ccacgcgccg cgcccgctcc aggtccgccg accatacgta gccggagagg
      541 ccgaagtccg tggcgttggc cagctcgatc gcgtgctcct cgtcgcggaa cggcgtgatg
      601 cagagtacgg gaccgaagat ctcctcgcgg aagatgcgca tgtcgggcgc gacgtcggcg
      661 aacaccgtgg gccgcacgaa gaaccccctg tcgaggcctt ccgggcgctc ggggccgccg
      721 gcgaccagcc gtgctccctc ggcggcgccg acgtcgatgt agttgcgcac cgtgtcgaac
      781 tgcttgcgcc ccgccaccgg gcccatgaag gtcgcctgat cgagaggatc gccgacggcg
      841 acgccgtcgg cggcgcgcgc ggcgaccgcg acggcctcct cgtagatctc ctccgcgacg
      901 agcatccgcg taggcgcgtt gcacgtctgc ccggagttgt gcatgcagcc gagcgtgccg
      961 gagcgcacga ccctctccac gttcacgtcg ccgaacacga tgttggcgga cttgccgccg
     1021 agttcctggg agacgcgctt gatcgtcggc gctgcggccg cggccaccag accgcccacg
     1081 gcggtggagc ccgtgatcga caccatgtcg acgagcggat gcgtcgcgaa cgcgtcgccc
     1141 agcgccgcgc cggcgccgtt caccatgttg aacacgcccg gtggcagccc ggcctcgtcg
     1201 gccgcctcgg cgaacagcag cgccgacagc ggcgagagct ggctcggctt gagcaccatg
     1261 gtgcagccgg ccgccagcgc cggcccgacc ttggccgtga cctggttcat gggccagttc
     1321 cacggcgcca gcagagcgca gacgccgacc ggctcgcgga cgatgcgcgt cgagccccag
     1381 tcctgctcca gcgggtagtc gcggagcgcc tccatggcct ctttgaggtg acgcgcgccg
     1441 gccagcacct ggccgccctg ggcgaggccg agcgggatgc cgacctcggt cgtcatcgcg
     1501 gcggcgatcg cggggatgcg gcgcttgtac gcctcgcgca gccgcgtcag cagctcgatg
     1561 cgctcctctc gcgtcgtctg cgagtaaccc gcgaaggccc ggtgggcggc ctgcaccgcc
     1621 gcatccacgt cggcgggaga ggcggccgct atcctggcag ccacctcctc cgtcgccggg
     1681 ttgacgacga cgatctcgtc accggggtgg gccggctcga cccactgccc gtcgatgtag
     1741 aacctggatc cgtcagtcac gcggcctccc agtcgatcga gcgcgccgga gccggtcagg
     1801 cgtccggcgg gcgatagcac atcctggtcc gcggcctact atccacccgt cgcgctcggg
     1861 agtccatcga gcgctccggg ctcaccgcct cttgctgtac gcttgccgcg atgcacgaga
     1921 cggggcgata gatgctggcg gcactgctcg cgctggccgc gagcttcacc tgggggacct
     1981 ccaactacct cgccggcgtc gagacgcgga cgcgctccgt gtggcacgtc gcggcgctga
     2041 gccagctcgc cgccgccgcc gcggcggcct tcggtctcgt ggcggcgacg cagccgccac
     2101 ccggcggctg ggacctcttc ctcctcgtcg tcaccgggat cgtcactgcg gccgggctgg
     2161 tgatgttcta caaggccctg gccatcggga cgatgagcgt ggtggcgccc atcatcgccg
     2221 cgcaggtgct gatcccggtg gcggccggga tcctgctcgg cgagcgtccc ggtccgcacg
     2281 cctacgtggg catggtggtc acggtcgccg gggtgctgct cgtgtcctgg gcgcgccggg
     2341 ggacgcgcga ccgcgtcgcc cgcaaggcgg tggtcctcgc tgcgctcacg gccgtcgcct
     2401 ggggcttcat gctgctcatc tacggcacca ccggtaagga gaacccggtg tggaccgtgt
     2461 tcgacacgcg ggtggcgtcc gcggtggtgc tcgccggcta cgtgctcctc accggccgcg
     2521 gcctctcgct caagggccag aacctgctgg tgctggcggc catcggcatc ctgctggcgg
     2581 ccgcgaactt cctgttcatc gtcgcgaccg gcctcgggta cctcagcgtc acgagcatcc
     2641 tcggctcgct gtcaccggtc gtcgtgacgg cgtacgccca ggtgctgttg cacgagcgcc
     2701 tggcgccgcg ccagtgggcg ggattcgtgg cggtgttcgc gggaatcgtg ctgctgtcgg
     2761 tgtgagggca gtgggcgctc tacgaggcga agcccgaggt tcgggaccgg cgcggtgcct
     2821 gaggacgccg gtttcaatgt caccttggtg tgaggcgacg cggccatctg actcgccggc
     2881 accgtgctac agttggacga ggaggtcacg tcatgcaact gaagttgact gcggtgttcg
     2941 aacaggtgcc tgagggctac atcggctacg ttgaggagct cccgggggcg aacacgcagg
     3001 gagccactct cgatgagacg cgtgagaacc tggttgaggc cgtcgagctc gttctcgatg
     3061 ccaaccggct gctcgccgaa gagcgactcg gagaccgaga cgtgattcgc gaaccgctgc
     3121 gcatctcagc atgaagcgcg cggacctcgt ccgccacctc gaaagccacg gctgcgaact
     3181 gctccgcgaa ggcgggcggc actcggtcta cgcgaaccgg gctgcaggca aggcgaccgc
     3241 cgtgccgaga catcgcgaga tcaacgactt cctcgcccgg aagatctgcc gggatatcga
     3301 ggtcgtggag ccctgaggtc cgcaggccgg ggccggccat cgagcggcgg gccggtgggc
     3361 gtcaggcctc cgcgccgcgc ttctcgaact tcttgcgctg ctcggtgctg aggatgtgct
     3421 tgcgtacgcg cacggtcttg ggcgtcacct cgacgagctc gtcgtcggcg atccactcga
     3481 gcgcgctctc cagggtgatc ggacgaggca ccttgagacc ggcgtcgatg tccttggtcg
     3541 acgaccggta gttgccgagt tgcttgcgct tggtggggtt gcagagcatg tcgtcgctgc
     3601 gtgagttctc gcccacgatc atgccctcgt agaccggggt catgttgccg acgaacagcg
     3661 tggcgcgctg ctgcaggttc tcgagcgagt agccggcggc ctcacccgcg gtctggctga
     3721 cgagcgagcc gcgctcgcgc ccggggagct cgcccttcca cgggccgtag cccgtgaagc
     3781 gcgagctcat cacgccgagg ccgcgcgtct cggtgaggaa gtcgttgcgg tagccgatca
     3841 ggccgcgcgt cgagatggtg aactcgaggc gcacgaggcc ggtgccggtg gactcgacgc
     3901 ccaccagctc gcccttgcgc aagctgagct cctggatgat cgcgccctgg tactcctccg
     3961 gcacgtcgat gatcagctgc tccatcggct cgagcagctt gccgtggccg tcgcggtggg
     4021 tgatgacctc ggggcgcgag acgcacagct cgaggccctc gcggcgcatc tcctcgatga
     4081 ggatcgcgag gtgcagctcg ccgcggccgc tcaccttgac gccgtcgggg cggccgaggt
     4141 cctcgacgcg cagcgcgacg ttgacgcgca gctcgcgctc cagccggtcc ttgatctgcc
     4201 gcagcgtcgc cgccttgccg tcctggccgg cgaacgggcc gttgttgacg aggaagaaca
     4261 tgctgaccgt cggctcctcg atctccagcg gcggaagcgc ggcggcggcg gccttgagct
     4321 ccggtgaggc gagggtgtcg ccgagcgtga tgtcggcggg gccggcgagc cagacgatgt
     4381 cgccggccga ggcctcctcg acctcgcggt tctcgaggcc gcgcgtgacc cagaggtgcg
     4441 tcacgcgcgc ggcctccgtg cccgtcatct cccagcccgc ctcggggtcg ccgatctcga
     4501 ggtgccgcgt ggtgatgcgc tgcagctcgt cgccgacctt gagcccgccg gcgagcacgc
     4561 gcccgcagcc gatgcggccg atgtagtcgc tccaggccag cgtgcttacc tgcatgagga
     4621 agggcgcgtc ctggtcgact ttgggcgcgg ggacactctc gatgatggtc tcgaagaggt
     4681 cgttcatgcc ggcgtcggcg agctcccgca acagcgccgg cgacaggccc ccgccgtcgt
     4741 gcagatgctc ttcgcgctcg tgctcttctt tcttgtactc gtcgatgtcg ttgacgatcc
     4801 agccctcgag gccggagccg tacagcaccg ggaagtcgag ttgctcgttg ttggcgccga
     4861 gctcgacgaa gaggtcgaag gtcttgtcca gcgcgcccga cggatcggcg ttcggccggt
     4921 cggccttgtt cacgatcacg atcggccgca gcccgaagct cagcgcgcgc atgagcacgt
     4981 agcgcgtctg cggcatgggg ccctcgttgg cgtccaccag cagcaggacg gagtccacca
     5041 tgctcagcac ccgctcgacc tcgccgctga agtcggcgtg gccgggggtg tcgatgatgt
     5101 tgatgaggta gtccttccac tccacggtgc agtgcttgga gcggatggtg atgccgcgct
     5161 cgcgctcgag atcgttgctg tccatcacgc gctcgtcgac gtgggcgttc tcgcggaaca
     5221 cgtgcgcagc gcggaagatg ctgtcgatca gcgtcgtctt gccgtggtcg atgtgggcga
     5281 tgatggcgag attgcggatc tgggaggcgg cgtgcacggg ggattctctc tgtcggggtg
     5341 gctggtgatg gaggcggggc tgcggctgcg gcctgacgcc cgaacgcccg gcagggctgc
     5401 gccggccggg cggacctttc tagtgtacac gcgcggaacg gcttcgtcgc ccgcgccctc
     5461 gtgcgaggac catggccatg cgcgtgcaca cgcacactca ctcacgggcg ccgcggcagt
     5521 atcatgggcg cagaggtgtg cgcgggacca cacgccgagc gccggacagc gagggcagcg
     5581 atgggcgagg aacccacgat ccgcatcgcg cagtcatgcg ccgcagagcc tcgcgtggcc
     5641 gttcgggagt tgtacgccgg cctggcgcag cccgagctgt cgctcgtgct gttcttctgc
     5701 tcgagcgact acgaccgcgg ggcgctcgcg gaagagttgc gcacacggtt cggccgcacg
     5761 ctggtggtgg gatgcaccac cgccggcgag atagggccgc tggggtgccg tgaccgcagc
     5821 ctgacaggcg tgagtttcgc cccatcggcc ggcaccgcgg tcatggggca tctcgacgat
     5881 ctccgggggt tcagcatccc ggagggcgtc gagttcgcgc gtagcctgcg gcggcggctc
     5941 gaggacgagg cgcagcaggc ggacgcgcac aacaccttcg ccctgctgct cgtcgacggg
     6001 ctcttcgggc acgaggagca gctggcccac gccctccagg agggactggg agagatcctg
     6061 ctggtcggag gttcggccgg cgacagcatg agcttcgcga acacgtacac ctaccgcgac
     6121 ggcgacttcg tgagcggctc cgccgtcctc gtcctgatca ccacgccgct tccgttcatc
     6181 gagttcaaga cgcagcactt cgagccgacg gacgaacggt tcgtggtgac cgaggcggac
     6241 cccgacctac gcgtggtcag cgagatcgac ggcctgccgg cggctgagga gtacgcacgg
     6301 atgctcggcg tggatccgcg ggacctcggc cccgcgcact tcgccgcatc gcccgtcgtc
     6361 gtcctcatcg acggcggcaa ctacgtgcgc tcgatccaga aggtgaaccc cgacggcagc
     6421 ctcaagttct actgcgctat cgagcgcgga gtcgtgctgc gcggggccaa gggcgtcgac
     6481 ctcgtccgca acctcgaggc ggccctcgca caggtgcgcg cccggatcgg cccgccgcag
     6541 gtcgtgctcg ccttcgactg catcctgcgc aggctggaga tcttgcagac gggggtggag
     6601 caacccgtcg ccgacctcct gagagccaac aacgccgtcg ggttcaacac ctacggcgag
     6661 cagtactgcg gtctccacat gaaccagacg ttgacggccg tcgccatcgg atcgcgccgg
     6721 gcggccggag gcgcgcgcaa tggctgacgc gcccgacacc cgtgacgtcg cgacgctgca
     6781 ggcggaggtc gcccggctca acaagatcgt cgacgcactg atggaccgct cggaagccgg
     6841 gatggacgtc aaggagtcgg acttcgggct gttccagacc acggtcagac tgcaagacca
     6901 ggtgcgccgc cacaccgaag agctcgaggc ggcgctgcgc gagcacgaag cgggccccgg
     6961 agaagccgat gcgtccgcgg cgcgcgacat gcagacgctg cggcggacgg ccgcgctcca
     7021 gatccagctg ctggagctcg tcgtgcagga gaaggacgtc ggagagctgg tcgaccgggt
     7081 cgcctccatc ctcgaggtcc ccgtcgtcct cttcgacacg caggggcagg tggtctgcgc
     7141 ctcgggccgc gacttgaagg acgacggcct cgtccgcggc ctgtgggagg cctacgagcg
     7201 cctgcgcggc ctgcccggcc cgcacgccgt cgtcgaccgt gaagacgacc gggtgtactt
     7261 ccgcgaggtc ttcatcatgg accgcgtcga gcgggtcctc gcggcggtcg tctcgcggcg
     7321 gcagccgagc gacttcgccg ccgcgtcgct gctgtacctg cagcagctcg tcacgctcga
     7381 actgctgcgc aagcgcgacg agctgaagat gcgacgacgg gtgcggcgcg gcctgctccg
     7441 cgacatcctc gcgggccgca gccccacccg ggacctgcgg atccgactcg aggagcaggg
     7501 tttcagcgcg accagcgtgc tgcgcctggc ggtcgtcgag cccgcggcgc ttcgcccatc
     7561 gtccgccggc acccttgccg gcaagaccgc ggacaagtcc accggcggag tgctgcgcgc
     7621 ccttgacgcc cggctcagcc agcgccgcat ccccttcctc agcctgtcca acgggccctc
     7681 cgtggtggtg ctcacggcgc ttcccgacgc ggggaccggc cccgcccgag acctgctcga
     7741 cgacctgcga gccgcagcga ccgctgcgac cgccgccccg gagcgactcg tcgccggctg
     7801 ttccgcgccg ctcgccggcg tcgagagcgc gccccgctgc ctgcagcagg cgcgcgcggc
     7861 gtgcatggcc gcccggcgct cgtcggagcc ggtcaaaggc gcggtcttcg atgagctcag
     7921 cggccacctc atcctgctcg acgggctcga ccagaaggcg ctgtccgaca tcgtccagcg
     7981 cacgttcggg cccctcctcg agtacgacgc cgcgcaccgc accagtctct acaagacgct
     8041 gtacaccctc ttcgagaacc acctggccgt gcagcagacc gccgacgagc tgcacatcca
     8101 ccgcaacacg ctgcagaagc ggacagcgca cgtcgaggaa ctgctcggca tcgacctcag
     8161 cgagctcgac gacatcgtcg acgtccgcct cgggctgcag gctgctgtac tcctgggcca
     8221 gcagcccagc tgaccggcgg gtgtgcacgc gccctgcctt ttcgcgcaaa gagtagtcac
     8281 gcagcgaatc gaccccggcg gcgatccgtc ccatgctcct cctgtacccg cgacgagccg
     8341 cggacgggcg accgtgctcg cggccccgct cccagaggag gaggccatgg cgaaagtgct
     8401 gatgatccac ccggacaagt gcaccgggtg ccggaactgc gagctcgcgt gctcgttcga
     8461 gcacgagcgg cggttccgcc cgtctgcatc ccgggtccag gccttcacgt gggagcgtga
     8521 aggcatctcg gtaccgatga tgtgccagca gtgcgacgac gccgcgtgcg tcaccgtctg
     8581 ccccaccggc gccatgcaca agagctcgac cacccaggcg atgatcgact gggacgacga
     8641 caagtgcatc cgctgtcgca tgtgcgtcat tgcgtgcccc ttcggcaacg cgcgctacga
     8701 cgccatgacc agcagcatct tcaaatgcga caactgcgac ggcgcgccgc agtgcgccgc
     8761 cttctgcccc accgcagccc tcgagtacgt ggacgacacg gcgcaggtca aagcgcgcaa
     8821 gaaggcctac gcggccaagt tcaaggacgc tttccaggag gtgagctgag atgtacggct
     8881 ggacgggcac cctcctgcgc gtggacctcg gctccggcaa ggtcacacgt gaggcgctcg
     8941 acccgcgggt cgcgcgcgag tacatcgggg cgcgcggcct cggcggctac atcatccgct
     9001 ccgaggtcga ccccaaggtc gacgcgctgt cgccggccaa caagctcgtg ttcgcggccg
     9061 gcccgctcac gggcaccttc gcgccgtcgg cagggcgcta caacgtctgc acgaagagcc
     9121 cgctcaacga gtcgctggcg gcgtccaact ccggcggcgc cttcggcccc gaactcaagt
     9181 acgccggctt cgacgccgtg atcgtcgagg gcaaggccgc caagccggtc tacctctgga
     9241 tcaacgacgc cgaggtcgag atccgcgacg cggccggcgt ctggggcatg aacgtgccgg
     9301 acgccacgga cgcgatccgc gcggccaccg acgacgaggc caaggtggcc tgcatcggcc
     9361 cggccggcga gcacctctcg ctgatcgcca acatcatgaa cgagatgcac cgcgccgcag
     9421 ggcgcaccgg cgtcggcgcg gtgatgggct ccaagaacct caaggccgtg gccgtggtcg
     9481 gcaacggccc cgtcgaggtc gcggatcccg acgcgttcct cgaagaggtc ctcaagggcc
     9541 gcaagatgat cttcgacgac ccggtcggcg gcaccggcct gcccgcctac ggcaccgagg
     9601 tgctggtcaa catcctgaac acggtcggcg gcctgccgac gcgcaactgg caggacggcc
     9661 acttcgccaa cgcggacaag accggcggcg aagcgctggc ggccaagcag ctggtgcgca
     9721 agaagggctg cttcagctgc atcatcagct gcggccgtgt gaccaaggtc gacgcccccg
     9781 gcttcgaggg catcggcgag gggccggagt acgagaccgc ctgggcctac ggcgccgact
     9841 gcggcgtgga caacctcgac gcgatcacca aggccaacta cctctgcaac gagtacggca
     9901 tggacaccat cgccgcgggc tcctcgatcg cctgcgccat ggagctctat cagcgcggca
     9961 tcatcacgag caaggacacc gacggtgtgg acctcacctg gggcaacgcc gaggcgatag
    10021 tcgagatgac gcgcaagatg ggcgagggcg agggcttcgg cgccaaggcg gcgcagggct
    10081 cgtaccgtct cgccgacggc tacggccatc ccgagtactc gatgaccacc aagaagcagg
    10141 agatgccggc ctacgacggc cgcgccatcc aaggcatcgg gctcaactac gccaccagca
    10201 accgcggcgg ctgccacgtg cgcgggtaca ccatctcgca cgaggtgctg ggcagcggcg
    10261 gcaacgccga cccgcacgtc accgagggca aagccggcct gggcatcgcc ttccagaacc
    10321 tcacggcggc gctcgacgcc acggggagct gcctgttcgc gaccttcgga ctcaccggcg
    10381 agcagctcgt caagacgctg gccacgctga ccggctacga ctacacgctg gaggagttcc
    10441 tgcaggtggg cgaccgcatc tggacgcagg agcggctgtg gaacatcgcg gcgggctaca
    10501 cgagcgccga cgacacgctg cccaggcgcc tgctcgagga gccgctcaag accggcgcct
    10561 ccaagggtca cgtcaacaag cttggcgaga tgctgcccga gtactacgag cagcgcgggt
    10621 acgacgagca cggcgtgccg accaaggcga agctcaagga gctctcgctc aaggcatgac
    10681 cgtcaagtac ttcgccacca tccgcgacct gacgcgggag acggagcgcc gggtcgacgg
    10741 ccggccgggc gacctccgcg agctgctcac ggagctcgcg gagcgctacg gccggccgtt
    10801 ccggcgggcc gtgctggacg gcgacgacct gcacggggag atcatcgtct tcgtcaacgg
    10861 gcgcaacgtg cggcatctcc agatgctgga cacgccgctt gcggacgagg acgagatcgc
    10921 gatcttcccg atgatcgccg gcggctgacg caggagaaga ccacaggccg tgcgggtgag
    10981 gcgcggaagg acgaatggcc ggcgacgagg ctccgcgccg gccgggaagg cccgatcgtg
    11041 agcgacaccc ggaccataga cacgagccga gtgacggccg tgctgcggcg ttacccccgg
    11101 cacgaccccg gcgagctcgt caacgtcctg cacgacctgc aggccgagtt ccgctacctg
    11161 ccggaggagg cgctgcgggc ggccgccgcc cacctcggcc tgtcgcgcac ccaggtctac
    11221 ggggtcgcca gcttctacca cggcttccac accgagccgc gcggcgagca cacctgcacc
    11281 gtgtgcgtgg gcaccgcctg ccacgtgcgc ggcgccccgc gcctgctcga gcagctcgag
    11341 cgcgacaccg gcatcgccgc cggcggcacc acgccggacc tgtcgctctc gatcgaggag
    11401 gtcggctgcg tcggcgcctg cgcgatcggg ccgctggtgc tgctggacgg cgagcaccac
    11461 gggcacatga cgcccgcgcg gctctcgcgc atggttgcca agaccgtcgc cggtaaggac
    11521 gagccgtgag cgcccggctc gcggacccgg cggcgctgga ggaccgccgc gaggcgctcg
    11581 gcgccgggcg ggacgcccgg cgccgggtcc tcgccgtgtg cgccggcacc ggctgccgcg
    11641 cccagggcgc gctcggcgtc gtggacgcgc tccgcgccga ggtcgaggcg cgcggcgtgg
    11701 ccggcgaggt ggaggtgcgc gccaccggct gcctcggctt ctgccaggcc agcccgctgg
    11761 tgctgctcgt gcccgacggc gtcgcctacc ggcacgtcga gccggccgac gccgccgacc
    11821 tcgtcgcggc caccgcggcc ggcgagctcg tccaccgcct gctctgcatc gacgcggacg
    11881 gctcctgcac cctgcaggac gagatcccct tctaccgcgc ccagcagcgc ctgctgctgg
    11941 gcgccagcgc gctgctcgac ccgacctcca tcgacgccta catcacgctc ggcggctacg
    12001 cggcgctggc caaggctctc gacatgggcc ccgaggccgt gctcgaggag gtcaaggcct
    12061 ccggcctgcg cggccgcggc ggcgccggct tccccacggg gcgcaagtgg gaggcctgcc
    12121 gccgcgccga gggcgagctc aagtacgtgg tctgcaacgc cgacgagggc gaccccgggg
    12181 cgttcatgga cgacgccctg ctcgagggca acccgcacgg cgtgctcgag ggcatggtca
    12241 tcggcgccta cgccatcggc gcccgcgagg gccacgtcta cgtgcgccag gagtacccgg
    12301 tcgccgtgga gcgcatcacg cgcgccatcg agcaggcgcg cgcgctgggc ctgctcggcg
    12361 agcgcatcct cggcagcgac ttctccttcg agctgcgcgt cagccgcggc ggcggcgcct
    12421 tcgtctgcgg cgaggcgagc gcgatgatgg cctcgctcga gggccgccgc ggggtgccgc
    12481 ggcggcgcct catccacctc agcgagcgcg gcctctgggg gcgccccacc aacctcaaca
    12541 acgtcgagac ctgggcgaac gtgccgcaga tcgtcctgaa cggcgccgac tggtaccgcg
    12601 ccatcggcac cgccggctcc ccgggcacca agatcttctc gctcgtcggc aaggtggcgc
    12661 gcaccggcct cgtcgaggtc cccatgggca ccaccctgcg cgagatcgtc ttcggcgccg
    12721 gcggcggcgt gccgaacggc aagcagttca aggcggtgca gaccggcggc ccctccggcg
    12781 gctgcctgcc ggcggacaag ctcgacctgc cggtcgactt cgacacgctc gccgccgagg
    12841 gctcgatgat gggctccggc ggcatgatcg tgatggacga aggcacctgc atggtcgacg
    12901 tcgcccggta cttcctgcgc ttcctggcgt ccgagtcgtg cggcaactgc acctcgtgcc
    12961 gcgacgggct cgccctgctg cgcgacctgc tcggcgacgt cagcgagggc cgcggcacgc
    13021 gggagacgat cacgctgatc gagcagctcg ccgacgcggt ggaggcgacc tcgctctgcg
    13081 ccctcggcac gagcggcgtg aacccggtgc tctccaccct gcgccacttc cgcgacgagt
    13141 acctcgcgca cgtggacgag aagcgctgcc cggcgggcgt gtgcaaggcg ctgatcagct
    13201 tcagcatcct cgaggagcgc tgcaccggct gccgggcgtg cgcgctcggg tgcccgcagg
    13261 gctgcatcag cggcgagaag aagcagctgc acgtgatcga cgcggaggac tgcatcaagt
    13321 gcggcatctg ccgcgacgtc tgccggttcg acgccgtgct ggtgcagtag gaggccgtcg
    13381 tggacgcaga gctcatcatc gacggc
//
LOCUS       DASUMX010000091         4159 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1174064, whole genome shotgun sequence.
ACCESSION   DASUMX010000091 DASUMX010000000
VERSION     DASUMX010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4159
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1174064"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1767)
                     /locus_tag="VFZ86_04400"
     CDS             complement(<1..1767)
                     /locus_tag="VFZ86_04400"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX5641566.1"
                     /translation="MPAERPVCDQDVRRRVTADLGTSFLLEAGAGTGKTRVLVDRYVN
                     CVLDPAQGTGDVRTVAAITFTEKAAGELRQRVREEFERLAGQAAGDAARAAVIQGALD
                     ALDDAPISTIHGFAGRLLREFPVEARVDPAFEQLDALGSDLERGRLWEEWLGELAAAD
                     PAAGSTRQKLARLLRAGVRLDWLRTLAVGPKGVFGERYDLDPQVELLPEPDLLCALAG
                     LAVPLDRLAEHCSVACSNTGDKGCIAAMSLVDAGRALLERPPADVDQIAAAVYALPAK
                     ATATAPGGNKGNWDQARGGKDELLARYREAVAEVLAAREAYAAFLTTLAVAVADAFSR
                     WAAATQLALGRLDFTDLLGCLRDLLKRDPAARAALQARFRYLLVDEFQDTDPLQAEIV
                     FFLCERSPDAQDWRDVVLEPGKLFVVGDPKQSIYRFRRADIGMYDQVKLLVAGQPGGA
                     GAVEAIRQNFRTTPAVVQWVNNVFADVFEQDQEQGRQPGYQWVEPYRPAAEGPRVAVL
                     LGEDLGTAAGAADAARQAEARALAALLLAMHGEEQHRWTVQDRAPGAGGDGEAWRSPR
                     WGDVALLFRATTGLETYEQAFRE"
     gene            complement(1757..>4159)
                     /locus_tag="VFZ86_04405"
     CDS             complement(1757..>4159)
                     /locus_tag="VFZ86_04405"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEX5641567.1"
                     /translation="VWRETSEAERPAFAGDGSLAVVSVSDERAELREAVRAVLGAVEG
                     GAAAWDCALVVPHSDAVELAAAALQEAGLPIACRVPDRSAGTRILTRLLDCIAPPAGE
                     PFARRALIDLVTAGPLREGVGAGETAIWLDEARQAGVVSGAEQWTTRLARRRRGLERR
                     LADLEARGVVAVDDDDEVSGKVEAVRQRLSAVRGLEAAARALVEVCGRPPAHASWGEW
                     AALFSGAVDAVFAPAAAVEASDAASRLQALTVLEETVDLSEAAAVLRDMLGAARVPRG
                     RPGRDGVAVLTPLELRGLSFHTVVFTALAEGGFPARGRPDPLLGDAERRRIGAALGVR
                     LPLAEQRDAESLQLFAFGCEAARERLILLAPRSGAADGRPRLPSRLLLRLASSAAGRP
                     VGMDEFLDGEPLRPVWRRLAGSPAFSDDAVWVDERERDVALLLALSAAGSHGIARAYT
                     AEVLGDAEAASRRFGAWRSARSVVPSPWDGLLGAPARTALAATHPFDGEMHPTRLERY
                     VSCPFSFLLRDVFGLQVPDEPGDALEMDAREFGTLAHDILQRAYEQVIAGELRRDEAL
                     TAVRAAWETCCAEAEGRGVTGAALSWEVRRELLLEDLLETVRLDPVFSDPGSRPVAVE
                     WRFGEAVARPVAVDLPLGRSVRFAGRLDRVDETPSGARVIDYKSGAGGTERARIKEGL
                     SVQLPVYRLALRQAGDKDYGAITCLYRLVTRRGGFEDLDLPQSEEASERRLRDLVTSS
                     LALVDEGLFPRTTRQRCDFCDVRYACGVSAWARARKREHDLLEPVCALQSAPCEEDPD
                     AG"
BASE COUNT          564 a         1584 c         1533 g          478 t
ORIGIN      
        1 ttcacggaac gcctgctcgt aggtctcgag cccggtggtc gcccggaaga gcagggcgac
       61 gtcgccccaa cgcggcgagc gccacgcctc gccgtcaccg ccggcgcccg gggcgcgatc
      121 ctgcacggtc cagcggtgct gttcctcgcc gtgcatggcg aggagcagcg cggccagcgc
      181 ccgcgcctcg gcctgccgcg ccgcgtccgc ggcccctgcg gccgtgccga ggtcctcgcc
      241 gaggagcacg gccacacgcg gtccctccgc ggccgggcgg tacggctcca cccactggta
      301 gcccggctgg cggccctgct cctggtcctg ttcgaacacg tccgcgaaca cgttgttgac
      361 ccactgcacc accgccggcg tggtgcggaa gttctggcgg atagcctcga ccgcccccgc
      421 gccgcccggc tgccccgcga ccaggagctt cacctggtcg tacatgccga tgtccgcgcg
      481 gcggaagcgg tagatggact gcttgggatc gccgaccacg aacagcttgc ccggctcgag
      541 gaccacgtcg cgccagtcct gcgcgtccgg cgagcgctcg cagaggaaga agacgatctc
      601 cgcctgcagc gggtcggtat cctggaactc gtcgaccagc aggtaacgga agcgggcctg
      661 cagcgccgcc cgggcggccg ggtcgcgctt gagcaggtcg cgcaggcacc cgagcagatc
      721 ggtgaagtcg aggcggccga gcgccagctg cgtcgcggcc gcccagcgcg agaacgcgtc
      781 ggcgacggcg acggcgagcg tggtgaggaa ggcggcgtag gcctcccggg cggccagcac
      841 ctccgcgacc gcctcgcggt accgggcgag gagctcgtcc ttgcccccgc gggcctggtc
      901 ccagttgccc ttgttgccgc cgggcgccgt ggccgtggcc ttggccggca gtgcgtagac
      961 cgcggccgcg atctggtcga cgtccgccgg cggccgctcc aggagggcgc ggccggcgtc
     1021 gacgaggctc atcgcggcga tgcagccctt gtcgccggtg ttggagcagg cgacggagca
     1081 gtgctcggcc agcctgtcca gtgggaccgc caaacccgcc agtgcgcaga ggaggtccgg
     1141 ctccggaagc agctcgacct gagggtcgag gtcgtagcgc tcgccgaaca cgcccttggg
     1201 acccacggcg agcgtccgca gccagtcgag ccgcacgccg gcccgcagga ggcgagcgag
     1261 cttctgccgg gtcgatcccg cggcgggatc ggcggcggcg agctcgccga gccactcctc
     1321 ccaaagacgg ccgcgctcga ggtcagaccc gagggcgtcg agctgctcga acgccgggtc
     1381 cacgcgagcc tcgacgggga actcgcgcag caggcggccg gcgaacccgt ggatggtgct
     1441 gattggcgcg tcgtcgagcg cgtcgagcgc gccctggatg accgccgcgc gggcggcgtc
     1501 tccggcggcc tgccccgcca accgctcgaa ctcctcgcgg acgcgctggc ggagctcacc
     1561 ggctgccttc tccgtgaacg tgatggccgc gaccgtgcgc acgtcgccgg tgccctgtgc
     1621 ggggtcgagg acgcagttca cgtagcggtc gacgagcacc cgggtcttcc cggtgccggc
     1681 gcccgcctcc aggaggaagg acgtgccgag gtcggcggtc acccgtcggc ggacgtcctg
     1741 gtcgcagacc ggccgctcag ccggcatcgg ggtcctcctc gcacggggct gactggagtg
     1801 cgcacacggg ctccagaagg tcgtgctcgc gcttgcgcgc cctggcccag gcggagacgc
     1861 cgcaggcgta gcgcacgtcg cagaagtcgc agcgctgccg cgtagtgcgc gggaacagtc
     1921 cttcgtcgac cagcgccagc gagctcgtga cgaggtcgcg caggcgacgc tcggacgctt
     1981 cttccgactg tggcaggtcg aggtcctcga agccgccacg gcgcgtgacg agccggtaca
     2041 ggcaggtgat ggcgccgtag tccttgtccc cggcctggcg cagcgcgagt cggtagaccg
     2101 ggagctgcac gctcaggccc tccttgatgc gggcgcgctc ggtcccgccg gcgccggact
     2161 tgtagtcgat gacacgggct ccggacgggg tctcgtcgac gcgatcgagc ctgccggcga
     2221 agcgcaccga gcggccgagc ggaagatcga cggcgaccgg ccgcgctacc gcctcgccga
     2281 agcgccattc cacggcgacg ggccggctcc ccggatccga gaacaccggg tcgaggcgca
     2341 ccgtctcgag caggtcctcc agcagcagct cgcgccggac ctcccaggag agcgcggccc
     2401 cggtgacgcc gcggccttcg gcctcggcgc agcacgtctc ccacgccgcg cgcaccgcgg
     2461 tcagggcctc gtcccggcgt aactcgccgg cgatcacctg ctcgtaggcg cgctggagga
     2521 tgtcgtgcgc cagcgtcccg aactccctgg cgtccatctc gagcgcgtcg cccggctcgt
     2581 cgggcacctg gagcccgaac acgtcgcgca gcaggaagga gaagggacag ctgacgtagc
     2641 gctcgaggcg cgtggggtgc atctcgccgt cgaacgggtg ggtggcggcg agcgcggtgc
     2701 gggctggtgc gccgagcagg ccgtcccaag ggctcggcac gacgctcctc gccgagcgcc
     2761 aggcgccgaa gcggcggctc gccgcctccg cgtcgcccag cacctcggcg gtgtacgcgc
     2821 gcgcgatgcc gtggctgccc gcagccgaga gcgcgagcag gagcgcgacg tcgcgctcgc
     2881 gttcgtccac ccagaccgcg tcgtcggaga aggccggcga gccggcgagc cgacgccaga
     2941 ccgggcgcag cggctcgccg tccaggaact cgtccatccc gacgggccgg ccggccgcgg
     3001 aggaagccag ccgcagcagc agtcgggagg gcagccgcgg ccgtccgtct gccgcgcccg
     3061 aacgcggcgc cagcaggatc agccgctcgc gggcagcctc gcagccgaac gcgaacagct
     3121 ggagcgactc ggcatcgcgc tgctccgcca gcggcagccg gacgccgagc gcggcgccga
     3181 tgcggcggcg ctcggcgtcg cccagcagag gatccggccg gccccgcgcc gggaagccac
     3241 cttcggcgag cgcggtgaag accacggtgt gaaagctgag gccgcgcaac tcgagcggcg
     3301 tgagcacggc gacgccgtcg cgaccggggc ggccgcgcgg gacgcgcgca gcgcccagca
     3361 tgtcgcgcag cacggccgcc gcctcgctga ggtcgaccgt ctcttccagg acggtcagcg
     3421 cctggagacg ggaggcggcg tcgctcgcct cgaccgcggc cgccggcgcg aagacggcgt
     3481 cgaccgcgcc cgaaaaaagg gcggcccact cgccccagga ggcgtgcgcc ggcggccggc
     3541 cgcacacctc cacgagggcg cgcgccgcag cctcgagccc gcgtaccgcc gagaggcgct
     3601 gccgcaccgc ctccaccttg ccgctcacct cgtcgtcgtc gtcgaccgcc acgacgcccc
     3661 tggcctcgag gtccgcaagc cgccgctcca ggccgcgacg gcgacgcgcc agtcgggtcg
     3721 tccactgctc ggccccggac accaccccgg cctgccgggc ctcgtccagc cagatcgccg
     3781 tctcgcccgc cccaacgcct tcgcggagcg gcccggccgt gaccaggtcg atgagcgcgc
     3841 gccgcgcgaa gggctctccg gcaggcggcg cgatgcagtc caggaggcgc gtgaggatgc
     3901 gcgtgcccgc cgagcggtcc ggcacgcggc acgcgatggg aagccccgcc tcctgcagag
     3961 cggccgccgc gagctcgacc gcatcgctgt gcgggaccac cagcgcgcag tcccaggcag
     4021 cggcgcctcc ctcgaccgct ccgagaacgg cacgcaccgc ctcgcgcagc tcggcgcgct
     4081 cgtcggagac ggagaccacc gccagtgacc cgtcgccggc gaacgccggc cgctcggcct
     4141 cggacgtctc ccgccacac
//
LOCUS       DASUMX010000092         4120 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_455732, whole genome shotgun sequence.
ACCESSION   DASUMX010000092 DASUMX010000000
VERSION     DASUMX010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4120
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455732"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="VFZ86_04410"
     CDS             complement(<1..212)
                     /locus_tag="VFZ86_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEX5641568.1"
                     /translation="MTEFRSGFVAVFGRPNVGKSTLVNALVGRKVSIVSDHPQTTRRR
                     ITGVVTLDDCQLVLLDLPGFQKPFDSL"
     gene            complement(205..633)
                     /locus_tag="VFZ86_04415"
     CDS             complement(205..633)
                     /locus_tag="VFZ86_04415"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF004064.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEX5641569.1"
                     /translation="MSRAPEPAERLLFREALAVAANAYAPYSGYAVGAVAVGPSGRSH
                     HGANVENAAYPAGLCAERAALAALVTHGERELRSVAVAARDGSDCLPCGLCLQTLAEF
                     GDPEIVARAGGELRVVRLSELLATPFALPEAGAPQGGGDD"
     gene            complement(630..1352)
                     /locus_tag="VFZ86_04420"
     CDS             complement(630..1352)
                     /locus_tag="VFZ86_04420"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEX5641570.1"
                     /translation="MSRRRGNLLTSFNYAFDGIVHALRHERNMWIHFGIAGLVLIAGL
                     FFALTRVEVIALLVAISFVLITEMFNTAIEHTVDLVTDEDDPRARIAKDVAAGAVLVA
                     AVNAVAVAYLVFYDKITSVPYTILSKLRGSPIDVTVIALFIVILAAIALKALTHRGTA
                     FHGGLPSVHAALAFAAWVAVTFVASNTTFALPISAITLFLAALVAQSRVQAKIHTVTE
                     VAVGAALGIAITALLFRVWYPL"
     gene            complement(1333..1854)
                     /gene="ybeY"
                     /locus_tag="VFZ86_04425"
     CDS             complement(1333..1854)
                     /gene="ybeY"
                     /locus_tag="VFZ86_04425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEX5641571.1"
                     /translation="MNVVEVVNGTRTAVDEELVARLVARVLEAEGVRGAEVAVAFVGE
                     ARIRALNREHRGLDEVTDVLSFPLEEWESPAAGAASGAPDLGDVAEAAVSGTGGPPLL
                     LGDIVVCARRALEQARGDGLPPSLEVAVLLAHGTLHLLGYDHETDAGQMALRQAEVLE
                     LVDWEGIVVAPSR"
     gene            complement(1851..2870)
                     /locus_tag="VFZ86_04430"
     CDS             complement(1851..2870)
                     /locus_tag="VFZ86_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012947814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEX5641572.1"
                     /translation="MAPVSRTVIDLDSNSEALTLAGEHDAHLKVLEQRLDCTLTLRGN
                     VLILEGETCDVDKARAAVDELLTVIHGGRPLNPAMVESLMDIAVNSSGKPSEVYGDVV
                     WTHRGRQVGPRTVNQKLYVDAIRGNTITFGIGPAGTGKTYLATAMAVQAFFGKQVGRI
                     ILTRPAVEAGESLGFLPGTVSEKVDPYFRPLFDALYDMIDGEKLAVLIEKGEIEVAPL
                     AFMRGRTLNDSFIILDEAQNTSPEQMKMFLTRLGFGSKMVVTGDVTQIDLPHSRTSGL
                     VTVSEVLDDIEDISIVQFDGHDVVRHKLVQRIVNAYKAHDDEVGAARAQRQAPSGPRR
                     EGPGS"
     gene            complement(2872..3330)
                     /locus_tag="VFZ86_04435"
     CDS             complement(2872..3330)
                     /locus_tag="VFZ86_04435"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEX5641573.1"
                     /translation="MRELGAIEQRIKDDATAALKAGDKRRREACNSFVAALKKERIDS
                     RKQPTEADELTVLKRERKRRAEAIELYDGAGRTDLADQERYEEELLKGFMPEELTDAE
                     LEVLVDEGIAATDAVTPKDMGKVMGWLKPKVAGRADGKKLSDLVRGRLGG"
     gene            complement(3327..3668)
                     /locus_tag="VFZ86_04440"
     CDS             complement(3327..3668)
                     /locus_tag="VFZ86_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006715884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HEX5641574.1"
                     /translation="MSECIFCRIAAGEIPADVVREDDQFVAFRDVRPLAPVHLLVIPR
                     EHVGSLDEAEALDGGASARALAFIAATARSAGVAESGYRVITNTGPDAGQEVMHLHWH
                     VIGGTRLGGMA"
     gene            complement(3661..>4120)
                     /locus_tag="VFZ86_04445"
     CDS             complement(3661..>4120)
                     /locus_tag="VFZ86_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641575.1"
                     /translation="AVDGLRDVPGGAMLSTDVIVGYPTEDEAAFARTLEALRSGLFGR
                     VHVFAYSPRPGTAAAGLPPLPAPAVKERMGRALAAAEAAALAARRAALGREAEVLVEE
                     RREGLWKGYSSDYVRYALRGRARRGDLVRAVADELTGDGVAGSVKESPNE"
BASE COUNT          568 a         1479 c         1481 g          592 t
ORIGIN      
        1 agcgagtcga acggcttctg gaacccggga aggtccagga gcaccagctg gcagtcgtcc
       61 agcgtgacga ccccggtgat gcgccgccgc gtggtctggg gatggtcgct gacgatgctg
      121 accttgcgcc ccacgagtgc gttgaccagc gtcgatttgc cgacgttggg acgcccgaag
      181 acggcgacga agccgctgcg gaactcagtc atcgccgcct ccctgcggcg cgcccgcctc
      241 gggcagcgcg aacggcgtcg cgagcagctc gctcaggcgc acgacgcgca gctcgccgcc
      301 ggcgcgggcg acgatctccg ggtcgccgaa ctccgccagc gtctgcaggc agaggccgca
      361 gggaaggcag tcggagccgt cccgggcggc gacggcgacg gagcgcagct cgcgctcgcc
      421 gtgggtgacg agcgcggcga gcgccgcccg ctccgcgcag agacccgccg ggtaggcggc
      481 gttctcgacg ttggcgccgt ggtggctgcg gcccgacggg cccacggcca cggcgcccac
      541 cgcgtatccc gagtacggcg cgtaggcgtt cgcggcgacg gccagtgcct cccggaagag
      601 gaggcgctcc gccggctccg gggcgcggct cacagggggt accagacccg aaagagaagc
      661 gcggtgatgg cgatgccgag cgccgcgccg accgccacct cggtgaccgt gtggatcttg
      721 gcctgcacgc ggctctgtgc cacgagcgcc gcgaggaaca gcgtgatcgc cgagatcggc
      781 agcgcgaacg tggtgttcga ggccacgaac gtgaccgcca cccaggcggc gaaggcgagc
      841 gccgcgtgca ccgacggcag gcctccgtga aaggcggtgc cgcggtgcgt caacgccttg
      901 agcgcgatcg ccgcgaggat gacgatgaac agggcgatca cggtcacgtc gatcggcgac
      961 ccgcgcagct tgctgaggat cgtgtacggg acgctggtga tcttgtcgta gaagacgagg
     1021 taggcgacgg ccacggcgtt gacggcggcg accaggacgg cgccggcggc cacgtccttg
     1081 gcgatgcggg cgcgcgggtc gtcctcgtcg gtgacgaggt ccaccgtgtg ctcgatcgcc
     1141 gtgttgaaca tctcggtgat gagcacgaac gagatggcca cgagcagcgc gatgacctcc
     1201 acgcgggtga gcgcgaagaa cagcccggcg atgaggacca gcccggcgat gccgaagtgg
     1261 atccacatgt tgcgctcgtg gcgcagcgcg tggacgatgc cgtcgaacgc gtagttgaag
     1321 ctggtgagca ggttaccgcg acggcgcgac aacgatgccc tcccagtcga cgagctccag
     1381 cacctcggcc tggcgcagcg ccatctggcc ggcgtcggtc tcgtggtcgt agccgagcag
     1441 gtgcagcgtg ccgtgcgcca ggagcacggc gacctcgagg gacggcggca ggccgtcgcc
     1501 ccgggcctgt tcgagggcgc ggcgcgcgca gacgacgatg tcgccgagga gcaggggcgg
     1561 cccgcccgtg ccggagacgg ccgcttcggc cacgtcgccg aggtcgggcg cacccgaggc
     1621 tgcgccggca gcaggcgact cccactcctc cagagggaag ctcagcacgt cggtcacctc
     1681 gtccaggccg cggtgctcgc ggttcagggc gcggatgcgc gcctcgccga cgaaggcgac
     1741 ggcgacctcg gcgccgcgca cgccctcggc ttcgagcacc ctggccacga ggcgggcgac
     1801 gagctcctcg tccaccgccg tgcgcgtgcc gttgacgacc tcgacgacgt tcacgagccc
     1861 ggcccctccc gacgcgggcc ggacggcgcc tggcgctgcg cccgcgccgc ccccacctcg
     1921 tcgtcgtgcg ccttgtaggc gttgacgatg cgctgcacca gcttgtggcg caccacgtcg
     1981 tggccgtcga actgcacgat gctgatgtcc tcgatgtcgt cgaggacctc ggacacggtg
     2041 acgagccccg aggtccggct gtgcggcagg tcgatctggg tcacgtcgcc ggtgacgacc
     2101 atcttgctgc cgaacccgag gcgtgtgagg aacatcttca tctgctccgg cgaggtgttc
     2161 tgcgcctcgt cgaggatgat gaacgagtcg ttgagcgtgc gcccgcgcat gaacgcgagg
     2221 ggcgcaacct cgatctcgcc cttctcgatg aggaccgcca gcttctcgcc gtcgatcatg
     2281 tcgtacaggg cgtcgaacag cggccggaag tacgggtcga ccttctcgct gaccgtcccc
     2341 ggcaggaagc cgaggctctc ccccgcctcc accgccgggc gcgtgaggat gatgcgcccg
     2401 acctgcttgc cgaagaacgc ctgcacggcc atggccgtgg cgaggtacgt cttgcccgtg
     2461 cccgccggcc cgatgccgaa ggtgatcgtg ttgccgcgga tggcgtcgac gtacagcttc
     2521 tggttgacgg tccgcgggcc cacctgacgg ccgcggtgcg tccacaccac gtcgccgtag
     2581 acctcggagg gcttgcccga cgagttgacg gcgatgtcca tgagcgactc caccatcgcc
     2641 gggttcagcg gccgcccccc gtggatgacg gtgagcagct cgtcgaccgc ggcgcgcgcc
     2701 ttgtccacgt cgcaggtctc gccctcgagg atcagcacgt tgccgcgcag ggtgagcgtg
     2761 cagtcgaggc gctgctcgag gaccttcaga tgcgcgtcgt gctcgcccgc gagcgtcagc
     2821 gcctcgctgt tgctgtccag gtcgatgacc gtgcgcgaga ccggcgccac ttcaaccccc
     2881 caggcggccg cggaccaggt cgctgagctt cttgccgtcg gcgcggccgg cgaccttggg
     2941 tttgagccag cccatcacct tgcccatgtc cttgggcgtg acggcgtccg tggcggcgat
     3001 gccctcgtcc accagcacct cgagctcggc gtcggtgagc tcctccggca tgaagccctt
     3061 gaggagctcc tcctcgtacc gctcctggtc ggcgaggtcc gtgcgcccgg ccccgtcgta
     3121 gagctcgatc gcctcggcgc gccgcttgcg ctcgcgcttg aggaccgtga gctcgtcggc
     3181 ctcggtgggc tgcttacggc tgtcgatacg ctccttcttg agcgccgcga cgaacgagtt
     3241 gcaggcctcg cggcgccgct tgtcgcccgc cttgagcgcc gcggtggcgt cgtccttgat
     3301 gcgctgttcg atagcgccga gctctctcat gccatgcctc ccagccgtgt cccgccgatg
     3361 acgtgccagt gcaggtgcat gacctcctgg ccggcgtcgg gacccgtgtt cgtgatgacc
     3421 ctgtagccgc tctccgccac gccggcggag cgggccgtgg cggcgatgaa cgcgagcgcg
     3481 cgggccgagg cgccgccgtc cagggcctcg gcctcgtcca gcgagccgac gtgctcgcgc
     3541 gggatgacca gcaggtgcac gggcgcgagc ggccgcacgt cgcggaaggc gacgaactgg
     3601 tcgtcctcgc gcacgacgtc ggcggggatc tcgcccgcgg cgatcctgca gaagatgcac
     3661 tcactcattc ggtgactcct tgacgctgcc ggccacgccg tcgcccgtca gctcgtcggc
     3721 gacggcgcgc accaggtcgc cgcgccgggc gcggccgcgc agcgcgtagc gcacgtagtc
     3781 tgaactgtac cccttccaga ggccctcgcg ccgctcctcg accaggacct ccgcctcgcg
     3841 cccgagcgcg gcgcggcgcg cggcgagggc ggcggcctcc gccgccgcca gcgcgcgccc
     3901 catgcgctcc ttgaccgcag gggccggcag gggcggcagt cccgccgccg ccgtccccgg
     3961 ccgcggcgag taggcgaaca cgtgcacgcg cccgaagagg ccgctgcgca gcgcctccag
     4021 cgtgcgcgcg aacgctgcct cgtcctccgt cgggtacccg acgatgacgt ctgtgctcag
     4081 catcgcccca ccgggcacgt cccggaggcc gtcgaccgcg
//
LOCUS       DASUMX010000093        27171 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1174704, whole genome shotgun sequence.
ACCESSION   DASUMX010000093 DASUMX010000000
VERSION     DASUMX010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 27171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27171
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1174704"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3000)
                     /locus_tag="VFZ86_04450"
     CDS             complement(<1..3000)
                     /locus_tag="VFZ86_04450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX5641576.1"
                     /translation="MAFRTRNRGAERGEVELLRGLIKLANQLQSSLTLDAIVHVIATA
                     LSDTFGFREASVYLREAGGDFAVHATVGEFPDFDKLLFERPVPKHIWNQLFLEKHQIG
                     SSFFVDHRHHQWTEEQRYYLPELDLGWRKADEWDSGDDLFVPLYDKDHELMGVLDLFD
                     PADRRLPSLEVVKSLEVFATHAAVAIENARQYEALEEATAQLQAQLALRHAILELSGA
                     LLATLEPREVFAQIAVLLKEIVDYDALEVRLVDETANELYSGFATDADEEQMRSWRSS
                     IEVGVSGWVVRHNEAQLVNDMLNDPRGALVPGTEWEPQASIIAPLSLGDAVLGVLALD
                     RLGERTFDEHELEPVTLFANLAAIAIHNARQYEEAERATKQLAEQLALTHELLSASGA
                     VLSSLEQTEVLEHIADTLKQIVDYDTMDIRLVDAQRGQLVALYARDEDDEKEIFAYPI
                     AIDEGISGWVVRHNEAQLINDMATDERAVHIPGTEQDEVQACILVPLSVGDEVIGIMT
                     MDRLGGRTFEDRELEPARLFANLAAIAIRNARQYDELETTSAKLEGQLDMQHELMSLS
                     TLLLSSLDQREVFPRITTMLKDIVDYDAMDIRLLDETTRELVCIYSRDINADLNDQFR
                     LSIDEGLAGWVVRHDEAQLVNDMARDPRGVHIPGTGEDVPQASIVVPLRVLGKVTGVL
                     TLDRLGGRTFADDDLEPVTLFANLAAIAIQNARTYEAMERQAISDGLTGIHNYRHFQE
                     SLHAEVSRAERYGDHFCLLMMDLDHFKAVNDTIGHQKGDDVLRAVAGVLRSCSRESDY
                     LARYGGEEFTMILPRTTLDEAETLAERIRASVATLDPGHPDLHVTMSIGVSAFPESAK
                     DSDGVLGAADAALLRAKAGGRNRVCLYTDAIADATAQLEGDLAALGRRFAAFIGLSEA
                     EAAGLVTALAVHETGAAVQDQVQAILGSDQTTAAAPSEVRQSAVEALVYGNERWDGAG
                     YPEGRRGAEIPRVARAFAVCRRWDPASH"
     gene            complement(3215..4045)
                     /locus_tag="VFZ86_04455"
     CDS             complement(3215..4045)
                     /locus_tag="VFZ86_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007935342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HEX5641577.1"
                     /translation="MDKLIITVAGVGAETTKEAQPGLPVTAAEIGADAARCREAGAAM
                     YHLHVRDAEAHPTQAEEVFAAAIAAIRERTDIIIQTSTGGAMGMTADERLQPLALGPE
                     MASLTTGTANFGDEVFFNDAALMSEFYRRMQGKGVRPEFEIFEAGQIDNALRLVKKHG
                     PAGPLLHWDFVLGVPGSMSGEPRNLIFLVDRIPEGSTWTCTGIGRWHMPVTMTALALG
                     GNVRLGFEDNIFLHRGVLAKENAELVGRVARIAREWDRPCATPDEARRILRLGEYARE
                     "
     gene            complement(4086..5354)
                     /locus_tag="VFZ86_04460"
     CDS             complement(4086..5354)
                     /locus_tag="VFZ86_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006421160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5641578.1"
                     /translation="MAKTVESAPKLNLTQSMAMLEDAQRLTPGGVGGIRRAYNFVVGE
                     YPVFITHGHGGHIVDVDGNEYIDMLCAYGPIILGYVEDEINDAAKAQMDKGFCFSLVQ
                     PLQNELEERLADIMPCGEQTILVKTGSDATNAAVRAMRAYTGRDKIARCGYHGWGDWC
                     VEHHGGVPQAVWELTKEFEYGDIADLERVFAENPDEIAGVVITPVGHPMAKPIIAPPP
                     GYLQAVVDLAHKHGAVVTFDEIRTGFRVAMGGASERYGVTPDLTTVGKAMANGYSIAA
                     LVGRREVMEVYVKGAFLSSTYFPNSLEMAASMKCLDILEREKVQDAIWERGTRFLDRL
                     GAIVADAGVPVTMSGIPPMPFLTFDTVDDHYRERRTEFYTQCIRRGLFVQPYHHWYIC
                     YRHTEADLTRALDVVEESLAYVAQSHPWQR"
     gene            complement(5767..6717)
                     /locus_tag="VFZ86_04465"
     CDS             complement(5767..6717)
                     /locus_tag="VFZ86_04465"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX5641579.1"
                     /translation="MSAGSHRLRGYAMVGGCAVMFGATGVWVGMTDLPASTVLSMRMA
                     LAAVLVALFGGAGRWLRQSRRPGARHRLLALGLLDAVQLYTFMLALRHLDVALAVFLS
                     YMSPIYIALVAPRLLRQRTEPVVIVALALAVVGIVVMLAPGLLDPALRVSALGVALGL
                     ASGVLLALFFLVAKTLSADVDGSTMLISNGVVVAVVMLPLGLIQWAGAGFAFTLTDFW
                     AVVGLAVFSTALGGTIFLHGMRYIPVQHTSIVGLLEPASAPVFALLFLAERPSLSTLL
                     GGALVLVGAVLVVLFGASEEGLAGSPEEAVGEAGATREAG"
     gene            complement(6761..7672)
                     /locus_tag="VFZ86_04470"
     CDS             complement(6761..7672)
                     /locus_tag="VFZ86_04470"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX5641580.1"
                     /translation="MSRAARGYLLVAGSFLIMGMIGALVDWATAPESALLVLRFATAG
                     LVLGVVFARRHPLAGAAERSVWPRLLLMAAIDAFTLLLFFVAIRGTSVAIGMFLQFLA
                     PVWVALIAPRIFRARTEAIVYPALAIALAGLAVILMPSLLGEGVTLSPWGVTAGLLAG
                     VGYAFFQLVVKDLTRRISAVTIVFTEAWLDALILLPLALWQTIGAGYQLTTRDLVAGV
                     VLGVVCTALAYLMWTYGMGMVKVQHSSILGYLEPVSAPIYALFLLGQGISIWTIGGGV
                     LIVVAGLLVVLLGEHEEDGAGSLEPAP"
     gene            complement(7725..8660)
                     /locus_tag="VFZ86_04475"
     CDS             complement(7725..8660)
                     /locus_tag="VFZ86_04475"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX5641581.1"
                     /translation="MSRDAPGRDLFGYAIVVVSYLGMAGSAPLVAWADAPEAIILCLR
                     MAFAALALGAVFLRRPMLADWRRPGAAWRLALMAAMSSLTLLLFFYAVRNTSVAIAMF
                     MLFLMPVWVALAAPRLFKSPREPIVWPALGLALAGLAVILVPDLLGEGVEVSLPGMLA
                     ALFTGFGYATYTISVKGLTKIVAPSTISMAEAAGDTLLVLPLAVWQFSSTGYHLDGKA
                     WIAAVVMGVFCTAIPYTLWIVGTKRVRVEHVTILGYIEPVAGPLYAVFLVGELPTVWT
                     VIGGALILGAGLLIVVFGRGEGDGSVAAAAEPEPL"
     gene            complement(8657..9562)
                     /locus_tag="VFZ86_04480"
     CDS             complement(8657..9562)
                     /locus_tag="VFZ86_04480"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX5641582.1"
                     /translation="MKASRDLAAYAMVAGCFILVGLTGSIVSWASAPGSVLLVLRFSI
                     AAAVLLAVFARRRPLKGVFRRDMWLKLLLMGVLDGGSLLAYFFAIRSVGVAVATFLLF
                     TQPVWVALLAPRIVKTPTERIVFASLGVALVGLGVILAPSLLGGGVHLPAIGLAVGLA
                     AGLCYAGFQLLVKGLTREFTSVTIVIVECTLDALVILPLAVWQLAGTGMSLTSRDLVA
                     AVVLGLLCTAVAYTMWVEGVSRLRVQHSAILGFLTPVVAPLFAWLLLGEAITVPTALG
                     GVLIVAAGVLVVVFGRGEVEGELPL"
     gene            complement(9559..10515)
                     /locus_tag="VFZ86_04485"
     CDS             complement(9559..10515)
                     /locus_tag="VFZ86_04485"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX5641583.1"
                     /translation="MSQGKPSRRGRGHEPGSPPPPRGDPAGYALVIGSYMLFGLTPVL
                     IAWVSIPVSLLLVLRYAIAAGILLIVFWRRRPLAGVMRPGVRRRFVLMAVLDASQALA
                     YFYAVRVIGVAAATFIYFLQPLWVALLAPRLLHVATERVVFVAMPLAFVGLGLVLGPS
                     FAGAASPSAAGVAAALLSGLGFALFQITIKRQTREVSSVSMVIVMCLLDGLLLLPLAL
                     WQAVGQGYALSTQDLIGVMLIGVFTTALAFTMWVEGVGRVRVQHSAILGLLNAVASPV
                     FAYLLLGQGLSGWVVAGGALILAAGALVVLRGAGDVEQGQPL"
     gene            complement(10535..11506)
                     /locus_tag="VFZ86_04490"
     CDS             complement(10535..11506)
                     /locus_tag="VFZ86_04490"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX5641584.1"
                     /translation="MSRRAASAPLAGAAPAGSGADAASPERLRGYLEVGLASLANGSI
                     GVIVTFADMPATMLLCLRMLFAAAALGLVVVATGSWRDLRKQGAPVRVLGISVSLCLN
                     LILYFLAIRSAGVAVAIFLSYLAPVYLAFVAPRILKERTEPVVYLALGIGLAGMALIL
                     VPGLLLEGAGLSAAGLFFGWAAGVMYAAYLLFAKSLRGLQVRSTPVVFTQSAFTAAVM
                     LVPGLLAVNAAQYDYTGVDLLMAALLGLVTTAVSFSLFMDGLHYIRVQHASIVAYVEP
                     VSAPLYALVFLGQVPSSWTIAGGALIVAAGILVVLYARGEPELEPLG"
     gene            complement(11503..12429)
                     /locus_tag="VFZ86_04495"
     CDS             complement(11503..12429)
                     /locus_tag="VFZ86_04495"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX5641585.1"
                     /translation="MSAPLGGSRRLRGYVEIASSSLILGTSATLIQISTMPASLLVVL
                     RMALAGVVLAGIFFLYGGVEEVRRSGRLRRMILLGFVVALEMIFYFISIRASSVTVGV
                     ALEYMAPVWVALSAPWLLHTRRQGIDMIAVAVAVGGMALLVLPALTAADGAVTVPGVV
                     FGLLAGLMFAAAMMLISSMNDTGVRGSTFALFYCAGSVLLLTPLAVWQTAASGYQLTW
                     TDTWIVLVSGFVYTALCFSLFSDGLRYVRVEHAGILGYLEPVTAPLWAFLLIGEAPPW
                     TTYAGGALIVAAGILAIVFGKGESEMVVEPLT"
     gene            complement(12562..14349)
                     /locus_tag="VFZ86_04500"
     CDS             complement(12562..14349)
                     /locus_tag="VFZ86_04500"
                     /inference="COORDINATES: protein motif:HMM:NF025925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HEX5641586.1"
                     /translation="MPKVFVEPIGITLAVDEQESLLEGLVRAQIDIPTDCAGRGTCGK
                     CLVRLGSGDLTAPTERELKRIPEKLRAEGWRLACQAYPRSARVSIEVRGTAGQRRILT
                     TSKLTHGAAHPAVCSRFVQLEPPTLADKRSDLERLDEALGGVEVPLHVLEELPATLRD
                     GKWKVTATLYGKRVIDVHPGERERECYGVAVDVGTSKVIAYLFDLRHGTMIDQEAVEN
                     PQMRYGEDVISRIAHAAQNHETAELAAAVRGGINANLAALYERQDVHPNHVCDMTVVG
                     NTAMHHLALGLSPVGLGQAPFAPAAAEPLTLRASELGIDMNPDGGVHFPPPIAGFVGS
                     DALAVVAATHLAGKKRPSMAIDIGTNTEIALAHDGRVTVTSCASGPAFEGYQIRNGMK
                     AVAGAIERVKIDAAGEATELATITGAEPIGICGSGVVDLLAGLVHAGVVDKSGRMQAG
                     HPRVRKGEEGVEYLLTTGPHGDIVFTQHDVRSLQLAKGAIHSGWELLMESLGVPLEQL
                     ETVYIAGAFGNYLDLAAAQYLGLFPPVSTRRVAFVGNAAGVGAQMALIDVRARRRMAR
                     VRDRIEFLDLATDKRFLDVFSRRLGFAGD"
     gene            complement(14477..15772)
                     /gene="ablA"
                     /locus_tag="VFZ86_04505"
     CDS             complement(14477..15772)
                     /gene="ablA"
                     /locus_tag="VFZ86_04505"
                     /EC_number="5.4.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018357940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050066 - lysine 2,3-aminomutase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0046440 - L-lysine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HEX5641587.1"
                     /translation="MIRDRKDIPRFSGVSDADWDDYRWQLRNRLTTTEDFESVLNLTD
                     AQRRDLDACMGKFRVSVTPYYASLMDPDDPTDPVRMQGVPTPAELVIHAEDIEDAVSE
                     DFDSPTPRITHRYPDRVLFVVTEMCSMYCRHCTRRRLVGGTEDIVAQAEIDNAITYIE
                     RATEVRDVLISGGDPLVLSDKQLESILERVSAIDHVEIIRIGSRMPVVCPQRITPELV
                     AMLRKYQPIYFNTHFNHPQEFTDESKIACDRLNDAGIPVGNQTVLMRGINDDARVMKK
                     LSHKLLQYRVKPYYYYQCDLAQGTAHFRTSVAKGIEIYESLRGHTTGFGVPTYIIDAI
                     GGGGKTPVYPNYVISQAPGQIILRNYEGVISKYTEPDDYVEGQCHCEGCDEDRERLGV
                     ASMFDASHGPTLSEVWDARMQKVVDRKNRMADVFGTARV"
     gene            complement(15907..17349)
                     /locus_tag="VFZ86_04510"
     CDS             complement(15907..17349)
                     /locus_tag="VFZ86_04510"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HEX5641588.1"
                     /translation="MTEPMMSANGAGAPGAQPEAARPILMTRARLALRVLSDEDVERV
                     HAAAVQLLGAEGATAEAAALSAPASFILAGRVPEHDVTLGAGFVWLAAGTATAGEAGV
                     PERVRRLAGGDSVPATATDLEGAVRLADALPEVAVVAGPPLRAAGVPAVSALARCLSG
                     TSKHVMAGLLGSAEDAEAAVRIAAAVAGSADEARRRPPLSLCGGAAALEAALVFARAG
                     LPVGVVLAPRDAAASPAPASSPSASARGPVDAPPSGFGLALVRHHAGVLAGCAAVQSA
                     VPGAPFFYVADPVAAGVAPAGPSASLFQLAAAQLAARVGLPLVAGGLRTTSHEPDWQA
                     CQQHAFASMSTAGSGTDVTGGAGLLGAATAFSPQQLVMDAEIFSWNAKIAAGIEVDDE
                     TMAVEAIRQVDIGGNFLGQRHTRKHMKDVWRPRLLDRSMWDAWIASGREGAYEKATAL
                     AGQLLAEHAAVPLGAEARGVIERIIAGPGL"
     gene            complement(17346..18782)
                     /locus_tag="VFZ86_04515"
     CDS             complement(17346..18782)
                     /locus_tag="VFZ86_04515"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HEX5641589.1"
                     /translation="MPAARLTVWDDAACWRVHEATLALLEETGVEVRHAGAREQLADA
                     GARVDGTRARIPAALVDAALQGAPRHFKITQRGTSGGEGHARRGELGGLELEDGRSYF
                     GTGSDCVYVSDPDTGERRPSTTADVEGMAALAEKLPDLDFVMSMGLPSDVPARVGDLA
                     QFAAMLAGTRKPLLTTAHDAVSLRRMKEMAAMCGEGRSFGCYAMPNPPLIHSSEALDK
                     VRACAELDIPLVYAPAPAAGTTAPASVTATIVVGNAETLSGLVVHQLARTGAPFVYGV
                     GCGAFDMRTAVDVYGAPEHFLGNAAATDLARFYGLPSFAYAAVADAKSFDEQWAAEAG
                     ITAVLGALSRATLLHDVGYLESGLQSSYETIVLGDELVGYARALLVDVGVDDEALALN
                     EIDAVGPGGSHLGRDYTRRHHRDTWRPRLFDRATHDRWVRSGATTLKERVTARVQELR
                     DEPRAFALGQTIRDRLQAVLAEAEGSSR"
     gene            complement(18782..20248)
                     /locus_tag="VFZ86_04520"
     CDS             complement(18782..20248)
                     /locus_tag="VFZ86_04520"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HEX5641590.1"
                     /translation="MTTRINPIAPRLWWDVLNPSELERIHEATLDVLETVGIRFPSER
                     ALDVLERNGCEVDRATRIAKLPRAVVMEAVSQVPDSYVLAGRDPACDMLIDGKHCYLS
                     NDGSGVFVWDHHKGEKRPSTKADAATSARFVDALPNISYYWGPVVTSQDVPPATKALH
                     DAEAVFANTSKHFQTVTTVGDKPARYLIEMAAVIAGGTAQLRERPILSFMQCAVDPLA
                     HDGPNLEANLVAAEHGLASGFMPMPLAAGTGPATLAGNLVVQNAEALSGVVLLELAYP
                     GVPAFFAGAPSVIDLKTGGYTGGSPEDYLLAGASTQLAHFYGVPMAMGTMATGAKEPD
                     WQAAVDDSLSTFASVMTSADMMNGAGLLNGSKILSYPHMVMETEIYGIVQKVADGIVV
                     DDETLALDVIKKVGPNGTYLAEKHTRQHMKEIWRPGVWDRTPHDTWLREGKKGALEKA
                     TEIADDILANYRPEPLPEDVLEELRAIVARADEELAGS"
     gene            complement(20533..21159)
                     /locus_tag="VFZ86_04525"
     CDS             complement(20533..21159)
                     /locus_tag="VFZ86_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HEX5641591.1"
                     /translation="MNDQLKQAIIDGNEAVALAMTQALVATGATPREILDEALLPGMD
                     VVGARMREGECFIPEVLLSARVMQACLDLLRPHMAAGEEAGMGSVVIGTVEGDLHDIG
                     KNLVGMLLSGAGFEVVNLGTGVSAAAFVAAVKEHQPKIVGMSALLTTTLPHMAETIAL
                     LKAEGLREAVKVMVGGAPVTAAYAAEIGADGYGANAGMAVERAKELVG"
     gene            complement(21352..22743)
                     /gene="mtbB"
                     /locus_tag="VFZ86_04530"
                     /pseudo
     CDS             complement(21352..22743)
                     /gene="mtbB"
                     /locus_tag="VFZ86_04530"
                     /inference="COORDINATES: protein motif:HMM:NF021053.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="[dimethylamine--corrinoid protein]
                     Co-methyltransferase"
     gene            complement(22934..23620)
                     /locus_tag="VFZ86_04535"
     CDS             complement(22934..23620)
                     /locus_tag="VFZ86_04535"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5641592.1"
                     /translation="MTAAKSDGRGKLRFPLEDPLTSLPATAQRILKAATRLLAERGFE
                     AVTLENVAAEAGVNKASIRYNFGNKAGMLMAVVDALIHDECLRIVADTENVPEEERLH
                     VAMLGIRRMITEADSFRGFFDILPHAFRDPELRERMFSLYVWWYQQNLRWLGLGGNKD
                     VMDSDMLMGLAELIAAIPDGLSVQAGLNPDGFDLSRPLATLEFLLRNSMEQLVAIAER
                     EAGQDAPATG"
     gene            complement(23782..24840)
                     /gene="ltaE"
                     /locus_tag="VFZ86_04540"
     CDS             complement(23782..24840)
                     /gene="ltaE"
                     /locus_tag="VFZ86_04540"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HEX5641593.1"
                     /translation="MRTIDLRSDTVTRPSPEMRAAMLEAEVGDDVLGDDPTVLALQEH
                     AAELLGKEAALYFPSGTMCNQAAVRALTGRGDEVFLHAQAHIVFYEQGGASALSQVQL
                     RVFDSPDGTLDLEKMEEYVHTDADVHWAPTRLVCLEQTHNHCGGVVLPLEHILAVRAF
                     CDRHGLGMHLDGARLFNAVAATGVSAADYAAPFDTVSVCLSKGLGAPVGSLLVTDAAT
                     LPAAARARKVLGGGMRQAGVIAAGGLYALRHNVARLADDHRRARRLAEALAELPGLDV
                     DLGSVQTNMVFAGTRGTGVPAAELVGLLAAEGVLCLDEAPWSVRFVTHLDVDDDDVEV
                     AVTAAAKALRTAVGRSPV"
     gene            complement(24939..25679)
                     /locus_tag="VFZ86_04545"
     CDS             complement(24939..25679)
                     /locus_tag="VFZ86_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HEX5641594.1"
                     /translation="MLPRRALIFLGLAWILVMLYPDPGMLVRSVRNVARPQIEPQAVA
                     ALAARLPDDPRAVEAYVLGRQVPYAYDWQSAGVPWYFPTTAEAVSAGRGDCESRAVVL
                     ASILTAKGIPNDLRVGLGHIWVDYPGKEASVLENEAVQVAGVEDGRFFLRWPEELRLG
                     EEISEQLAIHWTPAPAWRLVLLFAGLSLIVLWNALARVLAAGGLGPAGPPAVEPRSAR
                     RHGRSRREGGAPAASLRRARRVAPSQRS"
     gene            complement(25753..>27171)
                     /gene="guaA"
                     /locus_tag="VFZ86_04550"
     CDS             complement(25753..>27171)
                     /gene="guaA"
                     /locus_tag="VFZ86_04550"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEX5641595.1"
                     /translation="IERIRSYDPKGIILSGGPMSVHVEDAPRVNPELFDLGVPVLGIC
                     YGAQGMALELGGTVAQTGLNEFGKTVLSVQKQGLILDGMRGEEQCWMSHHDSITVPPA
                     GFDVLASTQAAPVAAMEDRERGFYAVQFHPEVVHTPKGMDILRAFLYKACGCAPAFTP
                     ESIIEESIRKIREQVGESKAILGLSGGVDSSVAALLMHRAIGDRLTCVFVDQGMMRMR
                     EADRVVETFSGTFGIPLVAVDASDRFLGKLEGVTEPERKRKIIGEEFIRCFEEEARKL
                     TDAKFLVQGTLYSDVIESGTRQAAKIKSHHNVGGLPEKMDFELVEPLRWLFKDEVRAV
                     GEELGLPEHIVWRQPFPGPGLAIRIIGEITRERVDVLQRADAIVQEEIRKAGLYRELW
                     QCFAILPVIRSVGVQGDERTYGYPIAIRAVTSDDAMTADWAHLPYELLETLSSRIINE
                     VPEVNRVVYDVSSKPPSTIEWE"
BASE COUNT         4085 a         9347 c         9800 g         3939 t
ORIGIN      
        1 gtgactggcc gggtcccacc gccggcagac ggcgaaggcg cgggccacgc gagggatctc
       61 cgccccgcgc cgcccctcgg ggtagcccgc gccgtcccag cgctcgttgc cgtacacgag
      121 cgcctccacg gcgctctgcc gcacctcgct cggcgccgcg gcggtggtct ggtcgctgcc
      181 gaggatagcc tgtacctggt cctggacggc ggcgccggtc tcgtggacgg ccagcgcggt
      241 gaccaggccg gccgcctccg cttcgctcag cccgatgaag gcggcgaagc ggcgccccag
      301 ggcggcgagg tcgccctcga gctgcgccgt cgcgtccgcg atcgcgtccg tgtacaggca
      361 gacgcggttg cgaccgccgg ccttggcgcg cagcagcgcg gcgtcggcgg cccccaggac
      421 cccgtcgctg tccttggccg actccgggaa cgcggagacg ccgatggaca tcgtcacgtg
      481 caggtcgggg tgcccggggt cgagcgtggc gacggaggcg cggatgcgct cggcgagcgt
      541 ctcggcctcg tcgagcgtgg tccgcggcag gatcatggtg aactcctcgc cgccgtagcg
      601 ggccaggtag tccgactcgc gcgagcagct gcgcagcacg ccggcgacgg cgcgcagcac
      661 gtcgtcgcct ttctggtggc cgatcgtgtc gttgaccgcc ttgaagtggt cgaggtccat
      721 catcaacagg cagaagtgat ctccataccg ctcggcccgg ctgacctcgg cgtgcagaga
      781 ctcctggaag tggcggtagt tgtggatccc ggtgaggccg tcactgatcg cctggcgctc
      841 catcgcctcg tacgtgcgcg cgttctggat ggcgatggcc gccaggttgg cgaacagcgt
      901 gacgggctcg aggtcgtcgt cggcgaaggt ccggccgccc agacggtcga gggtgagcac
      961 gccggtgacc ttgcccagta cgcgaagcgg cacgacgatg ctggcctgcg gcacgtcctc
     1021 gccggtgccc ggaatgtgca ccccgcgtgg gtcgcgcgcc atgtcattga ccagctgcgc
     1081 ttcgtcgtgc cgcacgaccc agccggccag gccctcgtcg atcgagaggc ggaactggtc
     1141 gttgagatcc gcgttgatgt cgcgcgagta gatgcacacc agctcgcgcg tcgtctcatc
     1201 gagcagccgg atgtccatcg cgtcgtagtc gacgatgtcc ttgagcatgg tggtgatgcg
     1261 cgggaacacc tcgcgctggt cgagcgagct cagcagcagc gtgctcaggc tcatcagctc
     1321 gtgctgcatg tccagctggc cctcgagctt ggccgacgtc gtctccagct cgtcgtactg
     1381 ccgcgcgttg cggatggcga tggcggccag gttggcgaag agccgggccg gctcgagttc
     1441 ccggtcttcg aaggtccggc cgccgagccg atccatggtc atgatgccga tgacctcatc
     1501 gccgacggag aggggaacga ggatgcaggc ctgcacctcg tcctgttccg tgcccgggat
     1561 gtggaccgcg cgctcgtcgg tcgccatgtc gttgatgagc tgcgcctcgt tgtgacggac
     1621 gacccagccg ctgatgccct cgtcgatcgc gatcgggtac gcgaagatct ccttctcgtc
     1681 gtcctcgtcg cgcgcgtaga gggcgacgag ctgtccgcgc tgggcgtcga cgaggcggat
     1741 gtccatggtg tcgtagtcga cgatctgctt gagcgtgtcg gcgatgtgct ccaggacctc
     1801 ggtctgctcg agcgacgaga gcacggcacc gctggcgctg agcagctcgt gggtgagcgc
     1861 cagctgttcc gcgagctgtt tcgtcgcccg ctcggcctcc tcgtactgcc gcgcgttgtg
     1921 gatggcgatg gcggcaagat tggcgaacag cgtgaccggc tcgagctcgt gctcgtcgaa
     1981 cgtgcgctct cccagacgat cgagcgcgag cacgccgagg accgcgtcgc ccagcgagag
     2041 cggcgcgatg atgctggcct gcggctccca ctccgtgccg ggcacgagcg cgccgcgtgg
     2101 gtcgttgagc atgtcattga ccagctgcgc ctcgttgtgc cgcacgaccc atccactgac
     2161 gccgacctcg atggagctgc gccaggacct catctgctcc tcgtccgcgt cggtggcgaa
     2221 tccactgtag agctcgttgg ccgtctcgtc gacgaggcgg acctccagcg cgtcgtaatc
     2281 gacgatctcc ttgaggagca ccgcgatctg ggcgaagacc tcgcgcggct cgagcgtggc
     2341 gagcagggcg ccgctgagct cgaggatcgc gtggcgcagg gccagctgcg cctgcagctg
     2401 cgccgtggcc tcctcgagcg cctcgtactg gcgcgcgttc tcgatcgcga ccgccgcgtg
     2461 cgtcgcgaac acctcgagcg acttgacgac ctcgaggctc ggcaggcggc ggtcggccgg
     2521 atcgaagagg tccagcacgc ccatcagttc gtgatccttg tcgtagagcg gcacgaagag
     2581 atcgtcgccg ctgtcccact cgtctgcttt gcgccacccg aggtcgagct ccggcaggta
     2641 gtagcgctgc tcctcggtcc actgatggtg ccggtgatcg acgaagaagg atgagccgat
     2701 ctggtgcttc tcgaggaaga gctggttcca gatgtgcttg gggaccggcc gctcgaagag
     2761 gagcttgtcg aaatcgggga actcaccgac ggtggcgtgg accgcgaagt cgccaccggc
     2821 ctcccggagg tagacgctcg cctcccggaa cccgaaggtg tcgctcagag ccgtggcgat
     2881 cacgtggacg atcgcatcga gcgtcaggct cgattgcagc tggttcgcca gcttgatcag
     2941 gccgcgcagc agctcaacct cgccacgctc ggccccgcgg ttcctggtcc tgaacgccat
     3001 cgctcccctg ctgctgctgt cacggccgtt ccgaagggtg cccaccagtc tacgcccgtg
     3061 ggtatgcccg cggcacggcg gcgttcgcct tgcctacgtg aatgcgtttc ggcagcgtcg
     3121 caggattgct tgagggggac atccgcctat tccggagccc gaggtcgggc gggcggggca
     3181 ctgaccgacg ccccgcccgc ccgacttctt cggctcattc ccgagcgtac tccccgagcc
     3241 tgaggatccg ccgcgcctcg tcgggggtcg cgcaggggcg gtcccactcc cgcgcgatcc
     3301 gcgcgacgcg gccgacgagc tcggcgttct ccttcgccag cacgccccgg tgcaggaaga
     3361 tgttgtcctc gaacccgagg cgcacgttgc cgccgagggc gagcgccgtc atcgtcaccg
     3421 gcatgtgcca gcggccgatg ccggtgcagg tccaggtcga cccctcgggg atgcggtcga
     3481 ccaggaagat gaggttgcgc ggctcgccgc tcatggagcc cggcaccccc agcacgaagt
     3541 cccagtgcag cagcgggccg gccgggccgt gcttcttgac cagcctgagg gcgttgtcga
     3601 tctggccggc ctcgaagatc tcgaactcgg ggcggacgcc cttgccctgc atgcgccggt
     3661 agaactcgct catcagcgcg gcgtcgttga agaacacctc gtcgccgaag ttggccgtgc
     3721 ccgtggtcag cgaggccatc tccggaccca gcgcgagcgg ctgcaggcgc tcgtcggcgg
     3781 tcatgcccat ggcgccgccg gtgctcgtct ggatgatgat gtcggtgcgc tcgcggatcg
     3841 ccgcgatggc cgccgcgaac acctcctccg cctgcgtcgg gtgcgcctcc gcgtcgcgca
     3901 cgtgcaggtg gtacatcgcg gcgccggcct cgcggcagcg cgccgcgtcg gcgccgatct
     3961 ccgccgccgt caccggcagc cccggctgcg cctccttggt ggtctcggcg ccgacgccgg
     4021 ccacggtgat gatcagcttg tccatctgcg accccctttc gaggacgaga acgtctgcgc
     4081 gcaggctatc tctgccaggg gtggctctgc gccacgtagg cgagcgactc ctcgaccacg
     4141 tcgagcgccc gggtcaggtc ggcctccgtg tgccggtagc agatgtacca gtggtggtac
     4201 ggctgcacga acagaccgcg gcggatgcac tgcgtgtaga actccgtgcg ccgctcccgg
     4261 tagtggtcgt cgacggtgtc gaaggtgagg aacggcatcg gcgggatgcc gctcatggtc
     4321 accggcaccc cggcgtcggc gacgatcgcc cccagcctgt cgaggaagcg cgtgccgcgc
     4381 tcccagatgg cgtcctgcac cttctcgcgc tcgaggatgt cgaggcactt catgctggcc
     4441 gccatctcca ggctgttggg gaagtacgtg gagctcagga aggcgccctt gacgtagacc
     4501 tccatgacct cgcgcctgcc gaccagcgcg gcgatcgagt agccgttggc catggccttg
     4561 ccgaccgtgg tgaggtccgg ggtcacgccg tagcgctcgc tggcgccgcc catggcgacg
     4621 cggaagccgg tgcggatctc gtcgaaggtg acgacggcgc cgtgcttgtg cgccaggtcg
     4681 acgaccgcct gcaggtaccc gggcggcggc gcgatgatcg gcttggccat ggggtggccg
     4741 accggggtga tcacgacccc ggcgatctcg tccgggttct cggcgaagac gcgctcgaga
     4801 tcggcgatgt cgccgtactc gaactccttg gtgagctccc acacggcctg cgggacgccg
     4861 ccgtgatgct cgacgcacca gtcgccccag ccgtggtagc cgcagcgcgc gatcttgtcg
     4921 cggccggtgt aggcgcgcat cgcgcgcacg gccgcgttgg tcgcgtccga gccggtcttg
     4981 acgaggatgg tctgctcgcc gcagggcatg atgtcggcga gccgctcctc gagctcgttc
     5041 tggagcggct gcacgaggct gaagcagaag cccttgtcca tctgcgcctt ggcggcgtcg
     5101 ttgatctcgt cctcgacgta gccgaggatg atcggcccgt aggcgcagag catgtcgatg
     5161 tactcgttgc cgtccacgtc gacgatgtgg ccgccgtggc cgtgggtgat gaagacgggg
     5221 tattcgccga cgacgaagtt gtaggcgcgg cggatgccgc cgacgccgcc gggggtgagg
     5281 cgctgggcgt cctcgagcat ggccatggac tgggtgagat tcagcttggg ggcgctttcg
     5341 acggtcttcg ccatgtgggt tcctttcagc cgggcgaggc gcggagggtg aacgctcgtc
     5401 tcgtcctccg atgcatgaga cagcgatggc tgcggttgga tctcagccgg ggtccggcca
     5461 gagccgcttg tagatgagga ggatggtcac tccgtctccc tccggagcct gtgcgacccg
     5521 gcgcaggtcg cctcgagtca gccgactgag cgtatcgaat acgccgcggc ttggcatccc
     5581 ctgcgtgtac gcgtgtggga gcgtggcgca ccgcgccgga cgccagcctg tacacccgcc
     5641 atgtcctcgt atcgccccgg gctcatgggt cgcccggagg taatgcgagg gcgggtggca
     5701 tcgtgggtgg cgccagatgc ccgggcgaca ccttccatgc ggctccgaga gctcggcggc
     5761 ggcggctcaa cccgcctcgc gcgtcgcccc ggcttcgccc acggcttcct cgggtgaccc
     5821 ggcgagtccc tcttcggaag cgccgaacag cacgaccagc acggcgccga caaggacgag
     5881 ggcgccgccg agcagcgtcg acagcgacgg tcgctctgcc aggaacagca gggcgaagac
     5941 cggtgcgctg gccggctcga gcagcccgac gatggaggtg tgctgcacgg ggatgtagcg
     6001 catgccgtgc aggaagatgg tgccgccgag ggcggtgctg aacacggcca gccccacgac
     6061 cgcccagaag tccgtgagcg tgaaagcgaa gccggcgccg gcccactgga tgaggccgag
     6121 cggcagcatc accaccgcta ctaccacccc gttggagatc agcatggtgc tgccgtccac
     6181 gtcggcgctg agggtcttgg cgaccaggaa gaagagcgcg agcagcacgc cggacgccag
     6241 gcccaacgcg acgcccaggg cggacactcg cagcgcaggg tcgaggaggc cgggggcgag
     6301 catgacgacg atgccgacca cggcgagcgc cagcgccacg atcacgacgg gctcggtgcg
     6361 ctgcctgagc agccgtggcg cgaccagggc gatgtagatc ggcgacatgt acgagaggaa
     6421 cacggccagc gccacgtcca ggtgtcgtag cgcgagcatg aacgtgtaga gctgcaccgc
     6481 gtcgagaagg ccgagcgcga gaaggcggtg gcgcgcgccg gggcgccgcg actggcgcag
     6541 ccagcggccg gcgccgccga agagcgcgac cagcacagcg gcgagcgcca tgcgcatgct
     6601 gagcacggtg ctcgcgggca ggtcggtcat gccgacccac acgccggtgg cgccgaacat
     6661 gacggcgcag ccgccgacca tggcatagcc cctgaggcgg tgcgagccgg cgctcacggc
     6721 gccccgccgg cggtggctgc cgagccggcg acggcgccgc tcacggcgcc ggctccagag
     6781 atccggcgcc gtcctcctcg tgctcaccca gcagcaccac gagcaggccg gcgaccacga
     6841 tgaggacgcc gccgccgatg gtccagatcg agatgccctg gccgagaagg aacaaggcgt
     6901 agatgggcgc cgacaccggc tccaggtagc cgaggatcga gctgtgctgc accttgacca
     6961 tgcccatccc gtaggtccac atcagatacg cgagcgccgt gcagaccacc ccgaggacca
     7021 cgccggccac caggtcgcgc gtcgtcagct ggtagcccgc gccgatcgtc tgccagagcg
     7081 ccagcggcag caggatgagc gcgtcgagcc aagcctcggt gaagacgatg gtgacggcag
     7141 agatccgcct ggtgagatcc ttgaccacga gctggaagaa ggcgtagccg acgccggcga
     7201 gcaggcccgc tgtcacgccc cacggcgaga gcgtgacgcc ctcgccgagg agtgacggca
     7261 tcaggatcac ggcgagcccg gcgagggcga tcgcgagcgc ggggtacacg atcgcctcgg
     7321 tccgggcgcg gaagatgcgc ggcgcgatga gcgccaccca aacgggcgcc aggaactgca
     7381 ggaacatgcc gatggcgacg ctcgtcccgc ggatggcgac gaagaagagc aggagcgtga
     7441 aggcgtcgat ggccgccatc agaagaaggc gcggccagac ggagcgctcc gccgcgccgg
     7501 cgagcgggtg gcggcgggcg aagacgacgc cgagcacgag ccccgcggtc gcgaagcgca
     7561 ggaccagcag ggcgctctcc ggcgcggtcg cccagtccac gagcgccccg atcatgccca
     7621 tgatgaggaa cgagccggcg accagcaggt agccgcgcgc ggcgcggctc atcgcgcggc
     7681 tcggcgcgcg agggcgagcg ggcggtcgtc ggggcaggcg gccatcagag cggctcgggc
     7741 tccgcggccg cggcgacgga gccgtcaccc tcgccgcggc cgaacaccac gatcagcagg
     7801 ccggcgccca ggatcagcgc gccaccgatc accgtccaga ccgtcgggag ctccccgacg
     7861 aggaagacgg cgtacagcgg tccggcgacc ggctcgatat agccgaggat cgtcacgtgc
     7921 tccacgcgca cgcgcttggt cccgacgatc cagagcgtgt acgggatcgc ggtgcagaag
     7981 acccccatga ccaccgcggc gatccaagcc ttgccgtcca ggtggtagcc ggtgctgctg
     8041 aactgccaga ccgccagcgg gaggacgagc agcgtgtctc cggcggcctc cgccatggag
     8101 atggtcgacg gagcgacgat cttcgtcagg cccttcaccg agatcgtgta ggtggcgtag
     8161 ccgaaaccgg tgaagagcgc ggccagcatg ccgggcagcg acacctcgac gccctcgccg
     8221 agaaggtcgg ggacgaggat caccgccagg ccggcgaggg cgaggccgag cgccggccag
     8281 acgatcggtt cgcgcggcga cttgaagagc cggggcgccg ccaaggccac ccacaccggc
     8341 atgaggaaga gcatgaacat ggcgatggcg acgctggtgt tgcgcaccgc gtagaagaac
     8401 agcagcaggg tgagcgacga catcgccgcc atgagggcga gcctccaggc ggcgcccgga
     8461 cggcgccagt cggcgagcat cgggcgccgc aggaacacgg cgcccagcgc cagcgccgcg
     8521 aacgccatcc gcaggcagag gatgatcgcc tcgggcgcgt ccgcccaggc gacgagcggg
     8581 gcgctgccgg ccatgccgag gtacgagacc accacgatgg cgtagccgaa caggtcgcgg
     8641 cctggtgcgt cgcggctcaa aggggcagct ctccctcgac ttcgccgcga ccgaacacga
     8701 cgacgaggac gccggccgcg acgatcagga caccgccgag cgcagtcggg acggtgatcg
     8761 cctcaccgag cagcagccag gcgaagagcg gggcgacgac cggcgtcagg aaccccagga
     8821 tggcgctgtg ctggacgcgg agccgggaca ccccttcgac ccacatcgtg taggcgacgg
     8881 ccgtacagag gaggcccaga acgacggccg ccaccaggtc acgagacgtg aggctcatcc
     8941 cggtgccggc cagctgccag acggcgagcg gtaggatgac cagcgcgtcg agcgtgcact
     9001 cgacgatgac gatggtgacg ctcgtgaact cgcgggtcag ccccttcacc agcagttgga
     9061 agcccgcgta gcagagaccg gcggcgagac cgacggccaa gccgatcgcc gggaggtgga
     9121 ccccaccacc cagcagcgac ggggccagga tcacgccgag cccgacgagg gcgacgccga
     9181 gggaggcgaa gacgatccgc tccgtgggcg tcttgacgat ccgtggcgcg aggagcgcga
     9241 cccacaccgg ctgggtgaag aggaggaagg tcgccacggc gacgccgacg gagcggatgg
     9301 cgaagaagta ggcgagcagg ctgccaccgt ccaggacgcc catgaggagc agcttgagcc
     9361 acatgtcgcg ccggaagacg cccttgaggg ggcgccggcg cgcgaacacc gcgagaagga
     9421 cggcggcggc gatgctgaag cgaaggacga gcagcacgct gcccggtgcg ctcgcccaac
     9481 tgacgatgct tccggtgagt cccacaagga tgaagcagcc cgcgaccatg gcgtaggcgg
     9541 ccagatcacg gctcgccttc acagcggctg tccctgctcc acgtcgccgg cgccgcgcag
     9601 caccacgagc gcaccggccg caaggatgag cgctcccccg gccaccaccc agccggagag
     9661 tccctggccg agaagcagat acgcgaacac gggcgaggcc acggcgttga gcaggcccag
     9721 aatggcgctg tgctgtaccc ggacccgccc gactccctcg acccacatgg tgaacgcgag
     9781 cgccgtggtg aacacgccga tgagcatgac gccgatcaga tcttgagtgc tcagagcgta
     9841 gccctggcca acggcttgcc agagggccag gggcaggagc aacagcccgt cgagcaggca
     9901 catcacgatc accatcgaca cgctggagac ctcgcgcgtc tgccgtttga tggtgatctg
     9961 gaagagggcg aacccgagcc cggagagcag agccgccgcg acaccggccg cggacggcga
    10021 cgcggcgccg gcgaaggacg ggccgaggac gagccccagc ccgacgaagg cgaggggcat
    10081 cgcgacgaac acgacgcgtt ccgtcgcgac gtgcaggagg cgcggcgcga gcagcgccac
    10141 ccacagcggc tgcaggaagt agatgaaggt ggccgccgcc acgccgatga cgcgcacggc
    10201 gtagaagtag gcgagcgcct gcgacgcgtc gagcacagcc atgaggacga accgccgccg
    10261 aacgccgggg cgcatcacgc ccgccagcgg ccggcggcgc cagaagacga tgagcaggat
    10321 cccagccgcg atcgcgtagc ggaggaccag caggaggctg acggggatgc tgacccaggc
    10381 gatcaggaca ggagtgaggc cgaacaacat gtaggacccg atgacgagtg cgtacccggc
    10441 cggatccccg cggggcggcg gcggcgaacc gggctcgtga cccctccccc tgcgtgacgg
    10501 cttgccctgg ctcacgcgct gttccccgag ccagtcaccc gagcggctcc agttcgggct
    10561 cgccgcgggc gtagagcacc accaggatgc ccgcggcgac gatcagcgcc ccgccggcga
    10621 tcgtccacga gctcggcacc tggccgagaa agacgagcgc gtagagcggg gcgctcaccg
    10681 gttccacgta ggcgacgatg ctcgcgtgct gcacgcggat gtagtgaagg ccgtccatga
    10741 acagactgaa gctcaccgcc gtcgtgacca ggccgagcag ggccgccatg aggaggtcga
    10801 cccccgtgta gtcatactgc gcggcgttga ccgccagcag gcccgggacg agcatcaccg
    10861 ccgcggtgaa cgcgctctgc gtgaagacca caggtgtgct gcgcacctgc aggccgcgca
    10921 ggctcttggc gaacagcagg taggcggcgt acatgacgcc tgcagcccaa ccgaagaaca
    10981 gcccggcggc cgaaagcccg gcgccctcga gcagcaggcc cggcacgagg atcagcgcca
    11041 tgccggccaa gccgatccca agtgcgaggt acacgacggg ctccgtcctc tccttgagga
    11101 tccgtggcgc gacgaaggcc aggtagaccg gcgccagata gctgaggaag atggcgaccg
    11161 ctacgcccgc ggagcggatg gcgaggaagt agaggatcag attgaggcac agcgagacgc
    11221 tgatccccag cacgcggacc ggcgcgcctt gcttgcgcag gtcgcgccag ctgccggtcg
    11281 ccaccacgac gaggccgagc gcggcggcgg cgaacagcat gcgcaggcag agcagcatgg
    11341 tcgccggcat gtcggcgaaa gtgacgatca cgccgatgga gccgttggcg agactggcca
    11401 aaccgacctc gaggtagccg cgcaggcgct ctggggacgc cgcgtcggcg ccggagccgg
    11461 cgggcgcagc tccggccaga ggggcggatg ccgcacgccg gctcacgtca acggctccac
    11521 gaccatctcc gactcgccct tgccgaagac gatggcgagg atgccggcgg ccacgatgag
    11581 cgcgccgcct gcgtacgtcg tccacggcgg cgcctcaccg atgagcagga acgcccagag
    11641 cggggccgtg accggctcga ggtagccgag gatccccgcg tgctccaccc gtacgtagcg
    11701 aagcccgtcg ctgaagaggc tgaagcagag cgccgtgtag acgaacccgc tgaccaggac
    11761 gatccaggtg tcggtccagg tgagctgata gccgctcgcg gccgtctgcc agacggcgag
    11821 cggggtgagc agcagcaccg agcccgcgca gtagaagagg gcgaaggtgg agccccgcac
    11881 accggtgtcg ttcatgctgc tgatcagcat catcgcagcg gcgaacatga ggccggcgag
    11941 cagcccgaag accaccccgg gcacggtgac ggcgccgtcg gcggcggtca atgcgggcag
    12001 cacgagcaga gccataccgc cgacggccac ggcgaccgcg atcatgtcga tgccctggcg
    12061 ccgcgtgtgc agcagccacg gcgccgacag cgcgacccac accggcgcca tgtactcgag
    12121 cgcgacgccg acggtgacgc tcgaggcgcg gatcgagatg aagtagaaga tcatctcgag
    12181 cgccacgacg aaccccagga ggatcatccg gcgcaggcgg ccggagcggc ggacctcctc
    12241 cacgccgccg tacaggaaga agattcccgc aagcacgacg ccggccagcg ccatacgcag
    12301 cacgaccagc aggctcgccg gcatcgtcga gatctggatg agcgtcgccg acgtgccgag
    12361 gatcagcgac gaggaagcga tctccacgta gccgcgaagt cgtctggatc caccgagagg
    12421 tgcgctcatg cgcgcatctc gttgccccgc ctgcggcgct cactgagggg tgtggctacg
    12481 ccgcacccct cgttgaccct gctgcgtcgg tcattgagga gtgcccctgc ggcgcacccc
    12541 tcgctgaatt gcctgcggcg ctcagtcgcc ggcgaagccg aggcgacggg agaagacatc
    12601 cagaaagcgc ttgtccgtag cgaggtccag gaactcgatg cggtcgcgaa cgcgggccat
    12661 ccggcgtcgt gccctgacgt cgatgagggc catctgggcg ccgacgcccg cggcgttacc
    12721 gacgaacgcg acgcgccggg tggagaccgg cgggaacagg ccgaggtact gcgccgcggc
    12781 caggtcgagg tagttgccga acgcccccgc gatgtacacg gtctcgagct gctccagcgg
    12841 gactcccagg ctctccatca gcaactccca gcctgaatgg atggcgccct tggcaagctg
    12901 caggctgcgc acgtcgtgct gggtgaagac gatgtcgccg tgcgggccgg tggtgagcag
    12961 gtactccacg ccctcttcgc ccttgcgcac ccgcgggtgg ccggcctgca tgcgcccgct
    13021 cttgtcgacg acgccggcgt gcacgagccc ggcgagcagg tcgacaacgc ccgagccgca
    13081 gatgccgatc ggctccgctc cggtgatggt ggcgagctcg gtggcttcgc cggcggcatc
    13141 gatcttgacg cgctcgattg cgccggcgac ggccttcatg ccgttgcgga tctgatagcc
    13201 ctcgaaggca gggccggacg cgcaactcgt gaccgtgacg cgcccgtcgt gggcgagggc
    13261 gatctccgtg ttggtgccga tgtcgatcgc catgctcggc cgcttcttgc ccgcgaggtg
    13321 cgtcgccgcg acgacggcga gcgcgtccga gccgacgaac ccggcgatgg gcggcggaaa
    13381 gtgcacgccg ccgtccgggt tcatgtcgat gccgagctcg ctcgcgcgca gcgtgagtgg
    13441 ctcggccgcc gccggggcga acggcgcctg gccaagaccg acgggcgaga ggccgagggc
    13501 gaggtggtgc atcgccgtgt tgccgacgac ggtcatgtcg cagacgtgat tggggtgcac
    13561 gtcctggcgc tcgtacaggg ccgccagatt ggcgttgatg ccgccgcgca cggccgccgc
    13621 gagttccgcc gtctcgtggt tctgcgccgc gtgcgcgatc cggctgatga cgtcctcgcc
    13681 gtagcgcatc tgcgggttct cgacggcctc ctggtcgatc atcgtgccgt ggcgcaggtc
    13741 gaacaggtag gcgatgacct tggaggtgcc gacgtcgacg gccacgccgt agcactcccg
    13801 ctcgcgttcg cccggatgca cgtcgatgac gcgcttgccg tacagggtgg cggtgacctt
    13861 ccatttgccg tcacgcagcg tcgcggggag ttcctcgagc acgtgcagcg ggacctcgac
    13921 gccgccgagg gcctcgtcga ggcgctccag gtcggagcgc ttgtcggcca gcgtcggcgg
    13981 ttccagctgt acgaagcggc tgcagacggc cgggtgcgcg gcgccgtgcg tcagcttgga
    14041 ggtggtgagg atgcgccgct gcccggcggt gccgcgcacc tcgatactga cgcgcgccga
    14101 gcgagggtac gcctggcagg ccaggcgcca gccctcggcg cgcagcttct ccgggatccg
    14161 cttgagctcg cgctcggtcg gggccgtgag gtcacccgag ccgagccgta cgaggcactt
    14221 gccgcaggtc ccgcggccgg cgcagtcggt ggggatgtcg atctgggcgc ggacgagacc
    14281 ctcgagcagg ctctcctgct cgtcgacggc cagcgtgatg ccgatgggct cgacgaagac
    14341 tttcggcacg ttctctcctt gcgtttccgt gaggtcaggc gggcggctct gccggccgcc
    14401 cgccccatcg tcgactctat cgccgaagtg cctcccgctc ctacccctcg ggcacggcgc
    14461 tcaggttcga gccgcctcag accctggctg tgccgaacac gtcggccata cggttcttgc
    14521 ggtcgacgac cttctgcata cgagcatccc agacctcgct gagcgtcggg ccgtgagacg
    14581 cgtcgaacat gctcgccaca ccgagtcgct cgcggtcctc atcgcatccc tcgcagtggc
    14641 actggccctc gacgtagtcg tcgggctccg tgtacttgct gatcacgcct tcgtagttgc
    14701 gcaggatgat ctggccgggg gcctggctga tgacgtagtt cgggtacacc ggcgtcttcc
    14761 cacccccgcc gatggcgtcg atgatgtagg tgggcacgcc gaaaccggtg gtgtgcccgc
    14821 gcagactttc gtagatctcg atgcccttgg cgacgctggt gcggaagtgg gccgtgccct
    14881 gcgcaagatc gcactggtag tagtagtacg gcttgacccg gtactgcagc agcttgtgcg
    14941 acagcttctt catcacccgg gcgtcgtcgt tgatgccgcg catgagcacc gtctggttcc
    15001 caacggggat gccggcgtcg ttgagccggt cgcaggcgat cttgctctcg tcggtgaact
    15061 cctgcgggtg gttgaagtgc gtgttgaagt agatgggctg gtacttcctg agcatcgcca
    15121 cgagctccgg ggtgatgcgc tgcggacaaa cgaccggcat gcgcgagccg atgcggatga
    15181 tctccacgtg gtcgatggcg ctcacgcgct cgaggatgct ctcgagctgc ttgtcgctga
    15241 gcaccagcgg gtcgccgccg ctgatgagca cgtcgcggac ctcggtggcg cgctcgatgt
    15301 acgtgatcgc gttgtcgatc tcggcttggg cgacgatgtc ctcggtgccc ccgaccaggc
    15361 gccggcgcgt gcagtgccgg cagtacatgc tgcacatctc ggtgacgacg aagagcacgc
    15421 ggtccgggta gcggtgggtg atacgcggcg tcggtgagtc gaagtcctcg gacacggcgt
    15481 cctcgatgtc ctcggcgtgg atcacgagct cggccggcgt cgggacgccc tgcatgcgca
    15541 ccgggtccgt cggatcgtcg gggtccatga gcgatgcata gtagggcgtg acgctgaccc
    15601 ggaacttgcc catgcaggcg tcgaggtctc tgcgctgcgc gtcggtgagg ttgagcacgc
    15661 tctcgaagtc ctcggtcgtg gtgaggcggt tgcgcagctg ccagcggtag tcgtcccagt
    15721 cggcgtcgct gacgccgctg aagcgcggta tgtccttgcg gtctctgatc atgtgagcgt
    15781 ctccttctcc gcggggcccc tgacccccgc ggctccctgc ttcaccgcta cgtccggcgg
    15841 ccacgccgcc ggaacgatga tgcaacgttg catacggatg ctacagcggg ctctcgcccg
    15901 cgtcctctac agtccgggtc cggcgatgat gcgttcgatc acgccgcgtg cctcggcgcc
    15961 gagcggcacg gcagcgtgct cggcgagcag ctgccccgca agcgcggtcg ccttctcgta
    16021 ggcgccttct ctgccggagg cgatccaggc gtcccacata ctgcgatcca gcagccgtgg
    16081 acgccacacg tccttcatgt gcttgcgggt gtggcgctgg ccgaggaagt tgccgccgat
    16141 gtcgacctgc cggatggcct cgacggccat ggtctcgtca tcgacctcga tgccggccgc
    16201 gatcttcgcg ttccagctga agatctcggc gtccatgacc agttgctgcg ggctgaaggc
    16261 ggtggcggcg ccgagcagcc cggctccgcc ggtcacgtcg gtgcccgaac cggcagtgct
    16321 catcgacgcg aacgcgtgct gctggcaggc ctgccagtcc ggctcgtggc tggtggtgcg
    16381 gaggccgccg gccacgaggg gcaggccgac gcgggccgcg agctgggcag cggcgagctg
    16441 gaagagcgac gccgagggac cggccggcgc gactccggcg gccaccgggt cggcgacgta
    16501 gaagaacggc gcgccgggga ccgcggactg gacggccgcg cagccggcga gcacgccggc
    16561 gtggtgacgg acaagggcga gtccgaagcc ggacggcggc gcgtccacgg gaccgcgggc
    16621 agacgccgac ggcgatgacg ccggagcggg cgaggcggcg gcgtcccgag gagccaggac
    16681 cacgccgacg ggaaggccgg cgcgggcgaa gacgagcgcc gcttcaagtg ctgccgcgcc
    16741 gccgcagagc gagagcggcg ggcggcggcg cgcctcgtcg gcggagccgg cgacggccgc
    16801 ggcgatccgc acggcggcct ccgcgtcctc ggcagagccg agcaggccgg ccatcacgtg
    16861 cttgctggtg cccgagaggc agcgtgcgag cgcgctcacg gccggcaccc cggccgcgcg
    16921 cagcggcggc ccggcgacga cggctacctc gggcagcgcg tcggccagcc ggacggctcc
    16981 ttcgaggtcg gtggccgtgg cggggacgga gtcgccgccg gcgaggcgac gcacccgctc
    17041 gggtacaccg gcctctccgg ccgtcgccgt gccggcggcc agccatacga agccggcgcc
    17101 gagcgtcacg tcgtgctccg gcacgcgacc ggcgagtatg aagctcgcgg gcgccgacag
    17161 agccgcagcc tcagctgtgg ccccctcggc gccgagcaac tggacggccg ctgcgtgcac
    17221 gcgctcgacg tcctcgtcgc tcagcacgcg caacgcgaga cgcgcccgcg tcatcaggat
    17281 cgggcgcgcc gcctcgggct gcgccccggg cgcgccggcc ccattcgcac tcatcatcgg
    17341 ctcggtcacc ggctgctccc ttcggcctcg gcgagcacgg cctgcagccg gtcgcggatc
    17401 gtctgcccga gcgcgaacgc gcgcggctcg tcgcgcaact cctggacacg cgcggtcacc
    17461 cgctccttga gcgtcgtcgc gcctgagcgc acccaccggt cgtgcgtcgc gcggtcgaag
    17521 aggcgcggcc gccacgtgtc gcggtggtgc cgccgcgtgt agtcgcgtcc caggtgacta
    17581 ccgcctgggc cgacggcgtc gatctcgttc aacgcgagcg cctcgtcgtc gacacccaca
    17641 tcgacgagca gcgcgcgggc gtacccgacc agctcgtcac caaggacgat ggtctcgtag
    17701 ctgctctgca agccgctctc gaggtagccc acatcgtgca gcagcgtggc gcgactgagg
    17761 gcgcccagca cggcggtgat gccggcttcg gcagcccact gctcgtcgaa gctcttggcg
    17821 tcggcgaccg cggcgtaggc gaagctcggc agcccgtaga accgggcgag gtcggtcgcg
    17881 gcggcattgc cgaggaagtg ctcgggcgcg ccgtacacgt cgaccgccgt ccgcatgtcg
    17941 aaggcgccgc agccgacccc atagacgaac ggcgcgccgg tccgcgcgag ctgatggacg
    18001 acgagcccgc tcagcgtctc ggcgttgccg acgacgatcg ttgcggtcac ggacgccggc
    18061 gccgtggtgc cggcggcggg ggctggggcg tagacgaggg ggatgtccag ctccgcgcag
    18121 gcgcgcacct tgtcgagcgc ctcgctcgag tggatcagcg gcggattggg catcgcgtag
    18181 cagccgaagc tgcgcccctc gccgcacatg gcggccatct ccttcatgcg gcgcagggag
    18241 accgcgtcgt gcgccgtcgt cagcaacggc ttgcgcgtgc cggcgagcat ggcggcgaac
    18301 tgggcgagat cgccgacccg cgccggcacg tcgctcggca ggcccatgct catgacgaag
    18361 tcgaggtcgg gcagcttctc ggcgagggcg gccatgccct cgacgtcggc ggtggtgctc
    18421 gggcggcgct cgccggtgtc cgggtcgctg acgtagacgc agtccgaccc ggtgccgaag
    18481 tagctccgcc cgtcctctag ctcgaggccg ccgagctccc cgcggcgggc gtggccttcg
    18541 ccgccggacg tgccgcgctg cgtgatcttg aagtggcgag gtgcgccctg cagagcggcg
    18601 tccaccaggg cggccgggat gcgggcgcgc gtgccgtcga cgcgcgcgcc cgcatccgcg
    18661 agctgctcgc gggcgccggc gtgacggacc tccacgccgg tctcctcgag cagcgcgagc
    18721 gtcgcctcgt gcacgcgcca gcaggccgcg tcgtcccaga cggtgaggcg ggccgccggc
    18781 atcagctgcc cgccagctcc tcatcggccc gcgccacgat ggcgcgcaac tcctccagga
    18841 cgtcctcggg aagcggttcc ggccggtagt tcgccaggat gtcgtcggcg atctccgtcg
    18901 ccttctccag ggcgcccttc ttgccctcgc gcagccaggt gtcatggggc gtgcggtccc
    18961 agaccccggg acgccagatc tccttcatgt gctgccgcgt gtgcttctcg gcaaggtagg
    19021 tgccgttcgg cccgaccttc ttgatcacgt cgagcgccag cgtctcatcg tccaccacga
    19081 tgccgtcggc gaccttctgc acgatcccgt agatctccgt ctccatgacc atgtgcgggt
    19141 agctgaggat cttggagccg ttcaggaggc cggcgccgtt catcatgtcg gcgctggtca
    19201 tcacgctggc gaacgtgctg agcgagtcgt cgacggccgc ctgccagtcg ggctccttgg
    19261 cgccggtggc catggtgccc atcgccatcg gcacgccgta gaagtgggcg agctgagtgg
    19321 aggcgccggc gaggaggtag tcctcgggcg agccgccggt gtagccgccg gtcttgaggt
    19381 cgatgacgct cggagcgccg gcgaagaacg ccggaacgcc cgggtaggcg agctcgagga
    19441 gcacgacgcc gctgagcgcc tcggcgttct ggacgacgag attgccggcc agcgtcgccg
    19501 gccccgtgcc cgccgcgagc ggcatgggca tgaagccgct ggccagcccg tgctcggcgg
    19561 ccacaaggtt ggcctcgagg ttggggccgt cgtgcgcgag cgggtccacg gcgcactgca
    19621 tgaagctgag gatgggacgc tcgcgcagct gggcggtgcc gccggcgatg acggccgcca
    19681 tctcgatgag gtagcgcgcc ggcttgtcgc cgacggtggt caccgtctgg aagtgcttgc
    19741 tcgtgttggc gaacactgcc tcggcgtcgt gcagcgcctt cgtggccggc ggcacatcct
    19801 ggctggtgac gacggggccc cagtagtagc tgatgttcgg cagcgcgtcg acgaagcgcg
    19861 cgctggtcgc cgcgtccgcc ttggtgctcg gtcgcttctc gcccttgtgg tggtcccaca
    19921 cgaagacgcc ggagccgtcg ttgctcaggt agcagtgctt gccgtcgatg agcatgtcgc
    19981 aggcggggtc gcggccggcg agcacgtagc tgtcagggac ctgcgagacg gcctccatga
    20041 cgaccgcgcg cgggagcttg gcgatccggg tcgcgcggtc cacctcgcag ccgtttctct
    20101 cgaggacgtc gagagcccgc tcgctcggaa agcggatgcc gaccgtctcg aggacgtcga
    20161 gcgtggcctc gtggatgcgc tcgagctcgc tgggattgag cacgtcccac cagagccgag
    20221 gggcgatggg attgatccgg gtggtcattg cggcaacctc gctgggatcc gacgacgaca
    20281 ggtcagggtg gagtcggtca gcgggcgggc ggtcgcggtg atgctcagtg ggagcccgtc
    20341 cgcggagagc ggaggagctt agaggccgca ggcgcggttg atcgtgctga tgctgatgct
    20401 ggatgcgtcg ggcacgggcc ggagtctatc accgccctcg cgcgcgggaa accgtcgcga
    20461 acgcagcgga gtgccgcgcg tgcagcagtt ttgtggtcga gcccctgtcg ccgccgagcg
    20521 gccggccgcg ccctacccca ccagctcctt cgcccgctcc accgccatgc cggcgttggc
    20581 gccgtagccg tcggcgccga tctccgcggc gtaggcggcc gtgaccgggg cgccgccgac
    20641 catcaccttg acggcctcgc gcaggccctc ggccttgagc agggcgatgg tctcggccat
    20701 gtgcggcagc gtggtcgtga gcagggcgct catgccgacg atcttcggct ggtgctcctt
    20761 caccgcggcg acgaaggcgg cggcgctcac cccggtgccg aggttgacga cctcgaagcc
    20821 ggccccggag aggagcatgc cgacgaggtt cttgccgatg tcgtgcaggt cgccctcgac
    20881 ggtgccgatc acgacgctgc ccatgcccgc ctcctcgccg gcggccatgt gcgggcgcag
    20941 caggtcgagg caggcctgca tgacgcgggc gctgagcagc acctcgggga tgaagcactc
    21001 gccctcgcgc atgcgcgccc cgaccacgtc catgcccggc aagagcgcct cgtcgaggat
    21061 ctcacggggc gtggcgccgg tggcgacgag cgcctgggtc atcgccaggg cgacggcctc
    21121 gttgccgtcg atgatggctt gtttgagctg gtcgttcatc tgctgctcct tcgaggcgat
    21181 gcctgcggtg aggtgggcga cgcggtcacc ccgggagtga gagtgaccgc gtcgcgcggc
    21241 gacggccgtg cggggggaca cggaggcgtc gcgagctggt gtctggaccc ggccggtcgt
    21301 cgcacggagc gccggccggg ctgggaatgc ggtggacggg cgggcatgcc gtcaggcgac
    21361 gggcgcgccg agcccggtct tggccttgag ccgctcgacc gagttgatgc ggatgccgag
    21421 caggtcggcg atgcgggcct tggcctcgag acccttggcg agacccggga ccgcggtgac
    21481 cacaccgatg tcgagctctt cgcgcacgtt gcgcatgacg gtcgagtcgg acaggtcgaa
    21541 cacgctcact ccgagcttgc cggcgacgta ctccttggcc tccttgagcc tcatcttgtt
    21601 gagctgcatc ctggccacca gatctccggc ggtcttgatg ccacccatgg acgaggccac
    21661 cgcgtgcgcc atccccatgc cgttcgggtc gccggtgccc acctacaacc cgtcggcctt
    21721 gccgacctcg atcatggcgg cgctcgcccg gctcaccatg tccatgggcg agacctcgaa
    21781 catcggcacg ccgccgacgc cgttgccgac gttggggtgg atggggatca cggcatcggc
    21841 cgtgcaggcc ttgacgaagg tcgtcgcccg ggccacgttc caggcgcagg acttcttgct
    21901 gttgttgttg accacgggcc cgaagatggc cgccccggcc tgctcgacca gcttcatctg
    21961 ctggtgcggc cacaggcccg cgaggcgcgt gcccttgtac tcgagctgcc cgtggaagcc
    22021 gagcaccatc tcggccgcca tgctcacctc gacgggcatg tccgtggtcg acgagatggt
    22081 ctcggtggcg cgcagcgtgg cgaggaactc ggcgtcgccg gtcgacgcga ccgtgtcgta
    22141 gttgatcccg tcggcgccgg tctcggcgaa catccgggac tgccacacga cgtcgtcgtg
    22201 gcacgcctcc agcgcctcct cctgcgtccg gcgcgcctcg gcgatcttgc ccaggggcag
    22261 caggtccgac gggttctcga actgcccgtc ggggcggtag tacagcccga ggttcggcat
    22321 gaacccgtag aacagcggaa acacggtggt gtgctggagc agttccatgt gctcctgctc
    22381 cacgctgacg atgaacttgc tcggcttgac ggagtagtcg atgtgaccga cctccatcga
    22441 gtcgaacgcg aaggcgcgct cgaagagtct cacggccgtc tcgcgcccga tgggcgcggc
    22501 gatgccgctg gagaggcgcg acgagatgag cgtgttggtg ccgccgtcct tggtcatcac
    22561 ggcctcgtgg ccgcgctcca ctccccagat gcggctggga cagcagaaca tgtcgacgag
    22621 gtagtcgatc tcgttctcgt cgaggaccgg gatcttggcc ttcgccgcag ccgcctccga
    22681 gccctcgacg atgtctctcc ggacctcgtc tctcgtcatg cgcacggtgc tgccgtcgcc
    22741 catgcgggtg accactgagc tcaacgcccc cacccccttc gacgcctctc cgactccccg
    22801 gcaagcagca gcggtcactc aagcccgcaa cgttacccac ctgggtggat acagcatagt
    22861 ccggaccgat cggctgtcaa gcagtgcgac gccgcacggg acgaccgtgc gtcgctcgag
    22921 cgcgccggcc gcgtcagccg gtcgccggcg cgtcctgccc cgcctctcgc tcggcgatcg
    22981 cgaccagctg ctccatcgag ttgcgcagca ggaactccag ggtcgccagt ggccggctca
    23041 ggtcgaagcc gtcgggattg aggcccgcct gcacggagag gccgtcgggg atggcggcga
    23101 tgagctccgc gagccccatg agcatgtccg agtccatgac gtccttgttg ccccccaggc
    23161 ccagccacct gaggttctgc tggtaccacc agacgtagag cgagaacatg cgctcgcgca
    23221 gttccgggtc ccggaacgcg tgcggcagga tgtcgaagaa cccccggaag ctgtccgcct
    23281 cggtgatcat ccgccggatc cccagcatgg cgacgtgcag acgctcctcc tccggcacgt
    23341 tctccgtgtc ggcgacgatg cgcaggcact cgtcgtggat cagggcgtcc acgacggcca
    23401 tgagcatgcc ggccttgttg ccgaagttgt agcggatgga cgccttgttc actcccgctt
    23461 cggcggcgac gttctcgagc gtgaccgcct cgaacccgcg ctcggccagc aggcgcgtcg
    23521 cggccttgag gatccgctgc gccgtcgccg gcaggctcgt cagggggtct tcgagcggga
    23581 agcgcagctt gccgcgcccg tcgctcttcg cagcagtcac cgcaccctcc ttcgccgcgg
    23641 agccgagtgg accgggtggc gactcagcaa acgcaggaca agtcatccac ccgggtggca
    23701 cacaaggggc agtgtacgcc gtcgggcgcg cgccgccaag tggacgcgct cggggccgcc
    23761 ggccggcgcg ccggcgacgc ctcagaccgg acttcggccc acggccgtgc ggagtgcctt
    23821 ggcggccgcc gtcacggcga cctcgacgtc gtcgtcgtcc acgtcgaggt gggtgacgaa
    23881 ccgcaccgac cacggcgcct cgtcgaggca gagcacgccc tccgcggcga ggagcccgac
    23941 cagctcggcg gccggcacgc ccgtcccccg ggtgccggcg aacaccatgt tcgtctgcac
    24001 gctgccgagg tcgacgtcca ggcccggcag ctcggccagc gcctccgcca ggcggcgtgc
    24061 gcgacggtga tcgtcggcca ggcgcgcgac gttgtggcgc agcgcgtaca ggccgccggc
    24121 ggcgatgacg ccggcctggc gcatgccgcc gcccaggacc ttgcgggcgc gcgccgccgc
    24181 cggcagcgtc gcggcgtccg tcaccagcag cgagcccacc ggcgccccca ggcccttcga
    24241 caggcagacg ctgacggtgt cgaacggcgc cgcgtagtcg gcggcgctca cgcccgtcgc
    24301 cgcgacggcg ttgaagagcc gcgcgccgtc gaggtgcatc ccgaggccgt gccggtcgca
    24361 gaacgcgcgc acggccagga tgtgctccag cggcagcacg acgccgccgc agtggttgtg
    24421 cgtctgctcg aggcagacca gacgcgtcgg cgcccagtgc acgtcggcgt cggtgtggac
    24481 gtactcctcc atcttctcga ggtcgagcgt cccgtcgggc gagtcgaaca cgcgcagctg
    24541 cacctggctc agcgccgagg cgccgccctg ctcgtagaac acgatgtgcg cctgggcgtg
    24601 gaggaagacc tcgtcgccgc gcccggtcag cgcgcgcacc gccgcctggt tgcacatggt
    24661 gccggacggg aagtacaggg cggcctcctt gccgaggagc tccgccgcgt gctcctgcag
    24721 ggcgaggacc gtcgggtcgt cgccgagcac gtcgtcgccg acctccgcct cgagcatcgc
    24781 cgcgcgcatc tcgggcgagg gccgcgtcac ggtgtcgctg cgcaggtcga tcgttctcat
    24841 gtgggagcct cctggccgcc ggagctgggg gtgcgcagcg agggtatcgc aggcggcggc
    24901 cgatgccgac ccggccgcgc cggcccgcga cgggcccgct acgagcgctg cgaaggagcc
    24961 acgcgcctcg cgcggcgcag cgacgccgcg ggcgcgccgc cttctcgccg ggaccggcca
    25021 tggcgacggg cgctccgcgg ctccacggcc ggcggtccgg cggggccgag cccgccggcc
    25081 gcgagcacgc gggcgagtgc gttccacagc acgatcagcg agaggccggc gaacagcagc
    25141 accagacgcc aggccggcgc cggggtccag tggatggcca gttgctcgga gatctcctcg
    25201 ccgaggcgga gctcctcggg ccagcgcagg aagaagcggc cgtcctcgac gccggcgacc
    25261 tggacggcct cgttctcgag cacgctggcc tccttacccg ggtagtcgac ccagatgtgg
    25321 ccgaggccga cgcgcaggtc gttggggatg cccttggcgg tgaggatgct ggccagcacg
    25381 acggcccggc tctcgcagtc gccgcggccg gcgctcacgg cctcggccgt cgtcgggaag
    25441 taccagggca cgcccgccga ctgccagtcg taggcgtacg ggacctgccg gcccagcacg
    25501 taggcctcga cggcgcgggg gtcgtccggc aggcgcgcgg ccagcgccgc gaccgcctgc
    25561 ggctcgatct gcgggcgggc gacgttgcgc acgctgcgca ccagcatccc cgggtccgga
    25621 tagagcatga cgaggatcca ggcgaggccg aggaagatga gcgcgcggcg cgggagcatg
    25681 tcctgatgat actcgcgtcc cgcgagcctg cgaccgggac ggccccgcgc accggtcagg
    25741 gcgcgacgag catcactccc actcgatggt gctcggcggc ttgctcgaga cgtcgtagac
    25801 gacgcggttg acctcgggca cctcgttgat gatgcgcgag gagagcgtct cgagcagctc
    25861 gtacggcagg tgcgcccagt cggcggtcat ggcgtcgtcg ctggtgaccg cgcgtatggc
    25921 gatggggtag ccgtaggtgc gctcgtcgcc ctgcacgccg acgctgcgga tcacgggcag
    25981 gatggcgaag cactgccaga gctcgcggta gaggccggcc ttgcggatct cctcctgcac
    26041 gatcgcgtcc gcgcgctgca gcacgtcgac gcgctcgcgc gtgatctcgc cgatgatgcg
    26101 gatcgcgagc ccgggacccg ggaagggctg ccgccagacg atgtgctccg gcagccccag
    26161 ctcctcgccc accgcgcgga cctcgtcctt gaacagccag cgcagcggct cgaccagctc
    26221 gaagtccatc ttctccggca ggccgcccac gttgtggtgg ctcttgatct tggccgcctg
    26281 ccgcgtgccg ctctcgatca cgtcgctgta gagcgtgccc tgcacgagga acttggcgtc
    26341 ggtgagcttg cgcgcctcct cctcgaaaca gcggatgaac tcctcgccga tgatcttgcg
    26401 cttgcgctcc ggctcggtga cgccctcgag cttgcccagg aaccggtcgc tggcgtcgac
    26461 ggccacgagc gggatgccga acgtgcccga gaacgtctcg accacgcggt cggcctcgcg
    26521 catgcgcatc atgccctggt cgacgaacac gcaggtgagc cggtcgccga tggcccggtg
    26581 catgagcagc gcggcgaccg acgagtcgac gccgccggag aggccgagga tcgccttgga
    26641 ctctccgacc tgctcgcgga tcttgcggat ggactcctcg atgatggact cgggcgtgaa
    26701 cgccggtgca cagccgcatg ccttgtagag gaacgcacga aggatgtcca tgcccttggg
    26761 cgtgtgcacg acctcggggt ggaactgcac ggcgtagaag ccacgctcgc ggtcctccat
    26821 cgcggccacc ggggcggcct gagtggaggc gagcacgtcg aagccggcgg gcggtaccgt
    26881 gatgctgtcg tggtggctca tccagcactg ctcttctccg cgcatgccgt cgaggatgag
    26941 cccctgcttc tgcaccgaca gcaccgtctt gccgaactcg ttgagaccag tctgcgcgac
    27001 ggttccgccg agctcgagcg ccatgccctg cgcgccgtag cagatgccga gcaccggcac
    27061 cccgaggtcg aagagttcgg ggttcacccg aggagcgtcc tcgacgtgga cgctcatggg
    27121 tccgccggag aggatgatgc ccttggggtc gtagctcctg atgcgctcga t
//
LOCUS       DASUMX010000094         8524 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_168973, whole genome shotgun sequence.
ACCESSION   DASUMX010000094 DASUMX010000000
VERSION     DASUMX010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 8524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8524
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168973"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(208..615)
                     /locus_tag="VFZ86_04555"
     CDS             complement(208..615)
                     /locus_tag="VFZ86_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017748861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX5641596.1"
                     /translation="MRVLVDSNVLLDVFEPDADWYEWSAESIVRCATHGRLVINHVVF
                     AEISVSFASIETLDGKLPRGLYERAPLPWEAAFLAGKAFLEYRRAGGTRRSPLPDFYI
                     GAHAAVTGMTLLTRDASRYRTYFPTVELIAPDA"
     gene            complement(619..849)
                     /locus_tag="VFZ86_04560"
     CDS             complement(619..849)
                     /locus_tag="VFZ86_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEX5641597.1"
                     /translation="MRVTTKGQVTIPQDIRETFGLLPHTEVEFVVDGERVVLRKASGE
                     NHEMRAWLDRARGAAEIKMTTDEIMALTRGED"
     gene            968..2296
                     /gene="ffh"
                     /locus_tag="VFZ86_04565"
     CDS             968..2296
                     /gene="ffh"
                     /locus_tag="VFZ86_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEX5641598.1"
                     /translation="MFDSLTERLQGVMQGLKGHGKLTAEDIDAAMREIRLALLEADVN
                     FKVVREFVAHVKERALGSEVMESLTPAQQVVKIVNEELVALMGSGDSKLAFSGHPPTV
                     VLMAGLQGSGKTTTCAKLAKLLKKDGKQPVLIAADVYRPAAIDQLQTLAGRVGVPCYA
                     PGADLDPVDIARDGVAFARQHGDVAIIDTAGRLHVDEELMAELVNIRDAVKPHNVLLV
                     VDAMTGQDAVNAADAFKGQVDLDGVVLTKLDGDARGGAALSVRAVTGKPIKYASTGEK
                     LDDFDVFHPDRMASRILGMGDVLSLIEKAEESLDEKKAAEMEAKLRRAEFTFEDFLDQ
                     LKQVRKMGSLSSILGMLPGVPGMKELKNVQVDDRQLDRIEAMVFSMTRQERRHPDIID
                     GSRRRRIAAGSGTTVQDVNNLLKQYREMQKMLKMFSSGKMKGFRLPGMSG"
     gene            2404..2637
                     /gene="rpsP"
                     /locus_tag="VFZ86_04570"
                     /pseudo
     CDS             2404..2637
                     /gene="rpsP"
                     /locus_tag="VFZ86_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005259388.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
     gene            2686..2919
                     /locus_tag="VFZ86_04575"
     CDS             2686..2919
                     /locus_tag="VFZ86_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005995515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEX5641599.1"
                     /translation="MLKDLLEYLAKSLVDNPDEVHVEATETDTTVVLELSVAKDDVGK
                     VIGKQGRIARALRTIVKASAVRDGKRAIVEIID"
     gene            3039..3551
                     /locus_tag="VFZ86_04580"
     CDS             3039..3551
                     /locus_tag="VFZ86_04580"
                     /inference="COORDINATES: protein motif:HMM:NF013907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEX5641600.1"
                     /translation="MTDSALWSCGTLGKVHGLKGEMYLNLAPGGFERLELGERFFVAD
                     EGAGEPRPCVLTRAGGTDRRPLVVLDLAATRDEAIKLQGLELLAAGDALDRQPHYRVG
                     DLLGLPVRTAAGRLVGEVRDVLEAPAHEILAVRAPDGGDVLIPLVDALISLQDGVLTV
                     VDGLLDEPEP"
     gene            3554..4213
                     /gene="trmD"
                     /locus_tag="VFZ86_04585"
     CDS             3554..4213
                     /gene="trmD"
                     /locus_tag="VFZ86_04585"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEX5641601.1"
                     /translation="MRFDVFTLFPGAFAWYLGQVHIRAAVGLGHEFVLTDYRDHTPLG
                     HQQVDDTPYGGGAGMVLRIDVVCAALEAVFGGAADGVKTSRRVVELTPKGRQLDDALA
                     TELAGGDLVLLCGRYEGIDERVEHLVSDRVSIGPYVLSGGEIAAMATLDAVTRKLPGV
                     ISNPDSVVSESHAPELEGGAEYPHYTRPAAFRGWEVPEVLRSGHHAEIARWRAEQVRK
                     R"
     gene            4311..4655
                     /gene="rplS"
                     /locus_tag="VFZ86_04590"
     CDS             4311..4655
                     /gene="rplS"
                     /locus_tag="VFZ86_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012360058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEX5641602.1"
                     /translation="MNIIENIEREQLRDDIPQFKAGDTVKVHFKVVEGTRHRIQVFQG
                     IVIKRQGQGVRETFTVRKQSFGVGVERTFPVHSPKIDHIEVTAIGDVRRAKLYYLRQK
                     VGKQARVREKQR"
     gene            4686..5246
                     /gene="lepB"
                     /locus_tag="VFZ86_04595"
     CDS             4686..5246
                     /gene="lepB"
                     /locus_tag="VFZ86_04595"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX5641603.1"
                     /translation="MKRVLEYVAVVVAAVLVALAVQAWVVKPYRIPSGSMLDTLRPGD
                     RVLVNRVVYHLREPERGDVIVFRYPQDPDVVFIKRVVGVPGDLLEARDGRLYVNGSKA
                     AEPYVHRTGGRLDPTVAQAAVSGSTLHDPWSLAKPYRVPAGNYFVMGDNRTDSDDSRD
                     WGTVPRSAIVGEGLATYWPVSRLRAL"
     gene            5277..5873
                     /gene="lepB"
                     /locus_tag="VFZ86_04600"
     CDS             5277..5873
                     /gene="lepB"
                     /locus_tag="VFZ86_04600"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX5641604.1"
                     /translation="MDHTQDAEPGKGGKKASTGRTILEYVVLAVVAIAVALLIQAFLV
                     KPYRIPSESMEDTLLIGDRVLVDRISWRFSDPSRQDIVVFHPPFIGPVLIKRVIGMPG
                     DEISLKDGDVYVNGERLDEPYVRTAGGETVPTEPFDNGLPWSLQQPYTVPDGNYFVMG
                     DNRVDSGDSREFGPVPRGQLVGKAFARYWPPGRIGGVD"
     gene            5891..6550
                     /locus_tag="VFZ86_04605"
     CDS             5891..6550
                     /locus_tag="VFZ86_04605"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEX5641605.1"
                     /translation="MQERFAFDLAAAGADGAAVAGADEAGRGCLAGPLAVAAVCLDYA
                     TLDEAGREALSGLDDSKRLTRPRREALYDEVLRLARQVVVVLFSPATIDRDGLHRCNL
                     RGLGRALERLDPAPDVALVDGFPLPECARDHRAVVGGDHLSAAIAAASIIAKVTRDRV
                     MRGLHASYPAYGFDHHVGYATAVHQTAICEHGVCELHRLSFASVAYQQLGLGLDATAD
                     C"
     gene            6683..7057
                     /locus_tag="VFZ86_04610"
     CDS             6683..7057
                     /locus_tag="VFZ86_04610"
                     /inference="COORDINATES: protein motif:HMM:NF009154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEX5641606.1"
                     /translation="MNARQQFGRAAEAAAARYLVREGWSLLGRNVRIGRGELDLIARR
                     GGVLAFVEVKARRSAAFGSPEDAVDGRKRRKVARLAELWLSCRPWALHGVSDVRFDIV
                     AVDASSQPPAVRHLPGAFTLDG"
     gene            7109..>8524
                     /locus_tag="VFZ86_04615"
     CDS             7109..>8524
                     /locus_tag="VFZ86_04615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEX5641607.1"
                     /translation="MLARVASEALSGLQGLPVVVEADVVAAAPAFSVVGLPDTSVQES
                     RERVRAAIVNSSYEFPSRRITVNLAPADLRKEGPSFDLPIALAFLLATGQVRDGSDGR
                     GPLAAVGELGLDGGLRPVAGALAVAQSLRSRGIRGLVLPAANAAEAALVPGLEVYPAS
                     SLAEAVAQIEAGGCGAAPPADPDALLAAAPVGDADFSDIVGQEGVKRALEIAVAGAHN
                     VLMTGPPGAGKTMLARRLPGIMPPLTRDEAIEITRIHSVAGLLPPGEPLVVRRPFRAP
                     HHTISSPGLVGGGGTPRPGEVSLAHLGVLFLDEFAEYRLSALEGLRQPLEDGEVTISR
                     RLTSVTYPARLMLVAAMNPCPCGFAGDKERECTCMGFRLQQYRSRLSGPLLDRIDLRL
                     DVPRVPPRARRDKRPSESSAAIRDRVVAARRRQLARLAGTGTYANAHMTTRQLRRFCG
                     LDAAATRKLDQAYDTMRLSARA"
BASE COUNT         1299 a         3016 c         2984 g         1225 t
ORIGIN      
        1 cgcgtgagca tgtcaggcta cggggcggga gacgccgatc ccaggggatg gccgaacgtc
       61 cttcacctct ccgtcggcga ggcgacgtct tagatcacct gcccggcccg ttccgggggc
      121 cggccggctt gacgctgtgg cgagcagaga gagagcgagt cgtgagagtc ggcgggcgcg
      181 tccgcgcgcc ggcccctgcg gaacgactca cgcatccggc gcgatgagct ccaccgtcgg
      241 gaagtaggtg cggtagcgcg acgcgtcccg ggtgaggagg gtcatgccgg tcaccgcggc
      301 gtgggcgccg atatagaagt cgggcagcgg ggacctccgg gtaccgccag cgcgccggta
      361 ctccaggaag gccttgccgg cgaggaaggc tgcctcccac gggagcgggg ctcgttcgta
      421 gaggccacgc ggcagctttc cgtccagcgt ctcgatactg gcgaagctga cggagatctc
      481 agcgaacacg acgtggttga tcacgagacg gccgtgggtc gcgcatcgga cgatgctttc
      541 ggccgaccac tcgtaccagt ccgcgtccgg ctcgaacacg tcgagcagca cgttgctgtc
      601 gacgagtacg cgcacaggtc agtcttcgcc gcgcgtgagc gccatgatct cgtcggtggt
      661 catcttgatc tcagcggcgc cgcgtgcgcg gtcaagccag gcgcgcatct cgtggttctc
      721 accactggcc ttgcggagga cgacccgctc tccatcgacg acgaattcga cctccgtatg
      781 gggaagaagc ccgaaggtct cgcggatgtc ttgagggatc gtgacttggc ctttggtcgt
      841 gacgcgcatg ctgcaacccc caggtagaga tactaccagt aagagtacga ccgtccttgc
      901 ctccgcgcaa ggcagcccca gagggcgtgc taccatggat gtcccgtaag cagacccagg
      961 gaacaggatg ttcgactcac tcaccgagcg cctccagggc gtcatgcagg gcctcaaggg
     1021 ccacggcaag ctcaccgccg aggacatcga cgccgccatg cgcgagatcc gcctggcact
     1081 gctcgaggcc gacgtgaact tcaaggtggt gcgcgagttc gtggcgcacg tcaaggagcg
     1141 cgcgctcggc tccgaggtca tggagagcct cacgccggcg cagcaggtcg tgaagatcgt
     1201 gaacgaggag ctcgtcgcgc tcatgggcag cggcgactcg aagctggcgt tcagcggcca
     1261 tccgccgacc gtcgtcctga tggccggcct gcagggctcg ggcaagacga ccacgtgcgc
     1321 caagctcgcc aagctgctca aaaaggacgg caagcagccg gtgctcatcg ccgccgacgt
     1381 ctaccgtccg gcggccatcg accagctcca gacgctcgcc gggcgcgtcg gcgtgccgtg
     1441 ttacgcgccc ggcgccgacc tcgacccggt cgacatcgcg cgtgacggcg tcgccttcgc
     1501 gcgccagcac ggcgacgtcg ccatcatcga caccgccggc cgcctgcacg tggacgagga
     1561 gctcatggcc gagctcgtca acatccgcga cgcggtcaag ccgcacaacg tgctcctcgt
     1621 cgtcgacgcc atgaccggcc aggacgcggt caacgccgcc gacgccttca aggggcaggt
     1681 cgacctggac ggcgtcgtgc tcaccaaact cgacggagac gcgcgcggcg gggccgcgct
     1741 gagcgtccgc gccgtcaccg gcaagcccat caagtacgcc agcaccggcg agaagctcga
     1801 cgacttcgac gtcttccacc ccgaccgcat ggcgagtcgc atcctgggca tgggcgacgt
     1861 gctgagcctc atcgagaagg cggaggagtc gctcgacgag aagaaggcgg cggagatgga
     1921 ggccaagctc cgccgcgccg agttcacctt cgaggacttc ctcgaccagc tcaagcaggt
     1981 gcgcaagatg ggctcgctct cgagcatcct cggcatgctg ccgggcgtgc ccggcatgaa
     2041 ggagctgaag aacgtccagg tcgacgaccg ccagctggac cgcatcgagg cgatggtctt
     2101 cagcatgacg cgccaggagc gccggcaccc ggacatcatc gacggcagcc gccggcgccg
     2161 catcgccgcc ggcagcggga ccaccgtcca ggacgtcaac aacctgctca agcagtaccg
     2221 cgagatgcag aagatgctga agatgttctc gagcggcaag atgaaagggt ttcgcctgcc
     2281 cggcatgagc gggtaaacta gcgattcttg cggcccgaag ccgggtcgca taaggttcac
     2341 acaccgctgc gaccctctgc ggtcgcagtg cgccgttcta ctaccctgag ggggtgacac
     2401 aagttggcag tacgaatgag attgacccgg gtagggagca ccaagaaccc tgtctaccgt
     2461 atcgtcgtcg tggactctcg cgcgcggcgc gacggtcgcg cgatcgagac catcggccag
     2521 tacaacccgc gcatggagcc gtcgctcatc gagatcgatg ctgagaaggc caagaagtgg
     2581 ctgggtgagg gcgcgcagcc ctcgcggacg gtcaagaagc tgctctcgat caagggcatc
     2641 gacaagtaag caagcccttg cggcgacgac ggagggagta gtgggatgtt gaaggacctg
     2701 ctcgagtatc tggccaagtc gctcgtcgac aacccggatg aggtccacgt ggaggctacg
     2761 gaaaccgaca ccaccgtcgt gttggagctc tcggtggcga aggacgacgt cggcaaggtc
     2821 atcggcaagc agggccgcat cgccagggcg ctgcgcacca tcgtcaaggc ctccgccgta
     2881 cgggacggca agcgcgccat cgtggagatc atcgattgac ccgcctgagg cggtctgggt
     2941 agtggcgcgc gacgcgcgcc tggctcagtc cgccgaagct gggtcaacgt gcggtgcacc
     3001 gaaggcacac cgcacactag ccactcgtca agatcctcat gactgactcc gccctttggt
     3061 cctgcggcac gctcggcaag gtgcacggcc tcaagggcga gatgtacctg aacctcgcgc
     3121 ccggaggctt cgagcgactg gagctgggcg agcggttctt cgtcgccgac gagggcgccg
     3181 gtgagccccg tccgtgcgtg ctcacgcgcg cgggcgggac ggaccggcgc cctctcgtcg
     3241 tgctggacct cgccgcgacc cgcgacgagg ccatcaagct gcaggggctc gagctcctcg
     3301 cggcgggcga cgccctcgac cggcagccgc actaccgcgt cggcgacctc ctggggctgc
     3361 ccgtgcggac cgcggccggc cgcctcgtcg gcgaggtgcg cgacgtgctc gaggcgcccg
     3421 cccacgagat cctggcggtg cgggcgccgg acggaggcga cgtgctcatc cccctcgtgg
     3481 atgcgctgat cagcctgcag gacggcgtcc tgaccgtcgt cgacggcctc ctcgacgagc
     3541 cggagccctg aacgtgcgct tcgacgtctt cacgctgttc cccggagcct tcgcgtggta
     3601 cctgggccag gtgcacatcc gtgcggcggt gggcctgggc cacgagttcg tgctcaccga
     3661 ctaccgcgac cacacgccgc tcggccacca gcaggtggac gacacgccgt acgggggcgg
     3721 cgcgggcatg gtgctgcgca tcgacgtcgt ctgcgccgcg ctcgaggccg tgttcggcgg
     3781 cgccgccgac ggcgtcaaga cgtcgcgccg cgtcgtcgag ctcacgccca aggggcggca
     3841 actggacgac gcgctggcca cggagctggc cggcggcgac ctggtgctgc tgtgcggtcg
     3901 ctacgagggc atcgacgagc gcgtggagca cctcgtcagc gaccgggtct ccatcggccc
     3961 ctacgtcctg tcgggcggcg agatcgccgc gatggcgacg ctcgacgcgg tcacgcgcaa
     4021 gcttcccggc gtcatcagca acccggacag cgtcgtgagc gagtcgcacg cgccggagct
     4081 ggagggcggg gcggagtacc cgcactacac gcgccccgcc gcgttccgcg gctgggaggt
     4141 gcccgaggtg ctgcgcagcg ggcaccacgc cgagatcgcg cgctggcggg cggagcaggt
     4201 cagaaagcgg taagatacct cttcgtggcc ggaggccggc agggagtttt gcgccgctcg
     4261 gcccgcgctg ctagactagc cgccgctttt ctgacgaaag acgagggtcc gtgaacatca
     4321 tcgagaacat cgagcgcgag cagctgcgcg acgacatccc gcagttcaag gccggcgaca
     4381 ccgtgaaggt ccacttcaag gtcgtcgagg gcacccgcca ccgcatccag gtcttccagg
     4441 gcatcgtgat caagcgccag ggccagggcg tgcgcgagac cttcaccgtg cgcaagcagt
     4501 cctttggcgt cggcgtcgag cgtacgttcc ccgtgcactc acccaagatc gaccacatcg
     4561 aggtcacggc catcggcgac gtgcggcgtg cgaagctgta ctacctgcgc cagaaggtcg
     4621 gcaagcaggc gcgggtccgc gagaagcagc gctgagctga cgcggccgct ccggccgtcc
     4681 gccccgtgaa gcgcgtcctc gagtacgtcg cggtcgtcgt cgccgccgtg ctggtggcgc
     4741 tggcggtgca ggcgtgggtg gtcaagccgt accgcatccc ttcggggtcg atgctcgaca
     4801 cgctccgccc gggcgaccgc gtgctcgtca accgcgtggt gtaccacctg cgcgagccgg
     4861 agcgcggcga cgtgatcgtg ttccgctacc cccaggaccc ggacgtggtg ttcatcaagc
     4921 gtgtcgtcgg cgtgcccggc gacctcctgg aggcgcggga cgggcgcctc tacgtgaacg
     4981 ggagcaaggc ggccgagccc tacgtccacc gcaccggggg ccgcctcgac cccacggtcg
     5041 cgcaggccgc cgtctccggc agcaccctgc acgatccctg gtccctcgca aagccgtacc
     5101 gcgtgcctgc gggcaactac ttcgtcatgg gcgacaaccg cacggacagc gacgacagcc
     5161 gcgactgggg aacggtcccg aggtcggcga tcgtgggcga aggcctggcg acgtactggc
     5221 cggtctcgcg cctgcgggcg ctctgataca ctcccgcccg gcggatcagg agaaagatgg
     5281 atcacacgca ggacgcggag cccggcaagg gcggcaagaa ggcctcgacg ggccgcacca
     5341 tcctcgagta cgtcgtgctg gccgtggtcg ccatcgccgt ggcgctgctc atccaggcgt
     5401 tcctcgtcaa gccctaccgc atcccctccg agtccatgga ggacaccctg ctgatcggcg
     5461 atcgcgtgct ggtcgaccgc atctcgtggc gcttctcgga tccgtcgcgg caggacatcg
     5521 tcgtcttcca cccaccgttc atcgggcccg tgctcatcaa gcgtgtgatc ggcatgcccg
     5581 gcgacgagat ctcgctcaaa gacggggacg tgtacgtcaa cggtgagagg ctggacgagc
     5641 cctacgtacg caccgccggc ggggagacgg taccgacgga gccgttcgac aacggcctcc
     5701 cgtggtctct ccagcagccc tacacggtcc ccgacggcaa ctacttcgtc atgggcgaca
     5761 accgcgtcga cagcggcgac agccgcgagt tcgggccggt gcctcgcggc cagctggtcg
     5821 gcaaggcgtt cgcccgctac tggccgcccg gccgcatcgg cggagtggat tgactgtcgg
     5881 aggcggggca gtgcaggagc gcttcgcctt cgacctcgcg gccgccggcg ccgacggcgc
     5941 ggccgtggcc ggcgccgacg aggccggccg tggctgtctg gccggcccgc tcgccgtcgc
     6001 cgccgtctgt ctcgactatg cgacgctcga cgaggccggc cgcgaagccc tctccggcct
     6061 cgacgactcc aagcgcctca cccgcccgcg tcgcgaggcg ctctacgatg aggtcctgcg
     6121 gctggcgcgc caggtcgtgg tggtgctctt ctctcccgcg accatcgacc gggacggcct
     6181 ccatcgctgc aacctgcgtg gtctcggccg ggcgctcgag cggctcgatc ccgcgcccga
     6241 cgtcgcgctg gtcgacggct tcccgctgcc cgagtgcgcg cgcgaccacc gcgccgtggt
     6301 cggcggcgac cacctctcgg ccgccatcgc cgcggcgtcg atcatcgcca aggtcacccg
     6361 cgaccgggtg atgcgcggcc tgcacgcgag ctacccggcc tacggcttcg accaccacgt
     6421 cggctacgcg acggcggtgc accagacggc gatctgcgag cacggcgtct gcgaactgca
     6481 tcgcctctcg ttcgccagtg tcgcctacca gcagctgggg ctgggactgg acgcgaccgc
     6541 ggactgctga cggcgtcctc cgcggccccg aacggccgca cggcgaggac acggttcgcc
     6601 gcgggtcgac gatccgcaac gtcatcccct gaccccgttc ggcaccctcc gcatcggccc
     6661 gcgcatgccc taggctggcc gggtgaacgc gaggcaacag ttcggacgcg cggcggaagc
     6721 cgcggcggcg cgctacctgg tccgagaggg gtggagcctc ctcgggcgca acgtgcgcat
     6781 cggccgcggc gagctcgacc tcatcgcgcg ccgcggaggc gtcctcgcgt tcgttgaggt
     6841 caaggcacgg cgttcggcgg cgttcggctc gccggaggac gcggtcgacg ggcgcaagcg
     6901 tcgcaaggtc gcccgtctcg ccgagctgtg gctctcctgc aggccctggg cccttcacgg
     6961 cgtgagcgac gtgcgcttcg acatcgtcgc cgtcgacgct tcgtcccagc cgccggcggt
     7021 ccgtcacctg ccgggcgcct tcacgctgga cggatgacgg cgccgccgtc aggaccggcc
     7081 tcgaatgatc ggcaccaggg agggtgacat gctcgcacgc gtcgccagtg aggcgctcag
     7141 cggccttcag ggcctgccgg tggtggtgga ggccgacgtg gtggccgcgg cgccggcgtt
     7201 cagcgtggtc ggccttccgg acacgtccgt gcaggagagc agggaacggg tgcgcgccgc
     7261 gatcgtcaac tcgtcctacg agttcccctc gcggcgcatc acggtcaacc tcgcgcccgc
     7321 ggacctgcgc aaggaaggcc cgtcgttcga cctgcccatc gcgctcgcct ttctgctcgc
     7381 gacgggacag gtgcgcgacg gctccgacgg ccggggcccg ctggccgccg tcggcgagct
     7441 cgggctcgac ggcgggttgc gccccgtggc gggggcgctg gccgtggccc agagcctgcg
     7501 gtctcggggc atccgcggcc tcgtgcttcc tgccgccaac gcggcggagg cggcgctcgt
     7561 gcccggactg gaagtctatc cggcgtcctc actcgccgag gcggtggcgc agatcgaggc
     7621 cggcggctgc ggagccgccc cgccggccga ccccgacgcg ctgctggccg ccgctcccgt
     7681 cggcgacgcc gacttctccg acatcgtcgg gcaggagggg gtcaagcgcg cgctcgagat
     7741 cgccgtggcc ggcgcgcaca acgtgctcat gacggggccg ccgggcgcgg gcaagaccat
     7801 gctcgcgcgg cggctgccgg gcatcatgcc gccgctcacc cgcgacgagg cgatcgagat
     7861 cacccgcatc cacagcgtcg cgggactgct gccgcccggg gagccgctgg tggtgcggcg
     7921 gcccttccgg gcgccgcacc acaccatctc ctctccgggg ctcgtcggcg gcggcggcac
     7981 gccgcgcccg ggcgaggtga gcctcgccca cctcggcgtg ctcttcctcg acgagttcgc
     8041 cgagtacagg ctctcggcgc tcgagggcct tcgccagccg ctcgaggacg gcgaggtgac
     8101 catcagccgc cggctcacgt cggtgacgta tcccgcgcgg ctgatgctcg tcgccgcgat
     8161 gaacccctgc ccctgcggct tcgccggtga caaggagcgc gagtgcacct gcatgggctt
     8221 tcgcctgcag cagtacaggt cccggctcag cggcccgctg ctcgaccgca tcgacctgcg
     8281 cctggacgtg ccgcgggtgc cgccgagagc acgtcgcgac aaacgcccgt ccgagtcctc
     8341 tgccgcgatc cgcgaccgtg tcgtggccgc gcggcgccgg cagctggcgc ggctcgccgg
     8401 cacggggacg tacgccaacg cgcacatgac cacgcgccag ttgcgccgct tctgcggcct
     8461 ggacgccgcc gccacgagga agctcgacca ggcctacgac accatgcgcc tcagcgcgcg
     8521 cgcc
//
LOCUS       DASUMX010000095        21001 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_169436, whole genome shotgun sequence.
ACCESSION   DASUMX010000095 DASUMX010000000
VERSION     DASUMX010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 21001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21001
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169436"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="VFZ86_04620"
     CDS             <1..500
                     /locus_tag="VFZ86_04620"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX5641608.1"
                     /translation="HLPDTLAQELLARLPDKREQDLRRLASHPEHTAGSLMTTDFVML
                     PRKATAGKALAWIRRERPEQHMMTYLYVVDAEERLVGVVSLRDLVLAAPDDQMTTFME
                     DDLVEVTADVDEEEVGRIMTKYDLLAIPVVDAERRLLGIVTLDDALEAVAPDDWKQRL
                     PRLYR"
     gene            complement(529..1128)
                     /locus_tag="VFZ86_04625"
     CDS             complement(529..1128)
                     /locus_tag="VFZ86_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641609.1"
                     /translation="MRAIDLTVASSDLLAPCCSTCTWWLTRPGITAGPGLRQEWEREA
                     EAEAGFFGRALLEGDAVIGWMHVAAARLVPHARALPAGPASPDAYLLTCAYFYDEEYL
                     HGFRFLLQEIEAALKHRRVAALEAFGLGHARGDDAFRGYLRELNLFNPDVLEGGGFRP
                     VQQKGEVARFRLDLAALIEAPRRSVAWEHVQAPAGTQPV"
     gene            complement(1125..1463)
                     /locus_tag="VFZ86_04630"
     CDS             complement(1125..1463)
                     /locus_tag="VFZ86_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641610.1"
                     /translation="MQQGQRPSISADVVTSAVWDAVKGLAGVRDLYRNPLQSLGERVH
                     LERHGPVRLDDGEDGPLLEIHLVVEAGSDLAAVGEAAARAGATYLTRTTGNPITRVEV
                     YVDDIADPST"
     gene            1614..3068
                     /locus_tag="VFZ86_04635"
     CDS             1614..3068
                     /locus_tag="VFZ86_04635"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5641611.1"
                     /translation="MTDTDRDITIYRADWVLPVAAPVLPDGGVAVGGDGRIVAVGPAP
                     RLIADHPGAAVVDTGRSIIMPGFVNCHSHMEYTTFRGILDDAEFGDWIISLVDVKASL
                     APDEYRISAQLGAMEALSSGITTVADTSYGAATLEAAGAAGLRGRLYLEVFGVDDARF
                     DETMADVVRRLDAAQAAAPATFEVGLAPHAPYTVSSRLYQAVSALARERGLKVMSHVA
                     ESQDELTYIRSGSGKFAHDYREKMGWERMLVQPYGVTPIKYLQQWDVLHPDFLAVHCV
                     NVTRDDIRILRAKDAAVAHCPKSNAKLGCGVAPLAGLLHEGVRVGLGTDSPASSNIMD
                     MFDEMRTMLFLHRAVERDVGVLDAQRCVRIATLGGAEALGMEAAVGSLEPGKWADLIA
                     VDVSRSHFAPIADPYSALVYGANQDDVTLTVVGGRELYRDRIHVDVDADAIRARAVAV
                     REKLQDRVLTGAVEVGGADSGWWHVAPVHREESV"
     gene            3069..3728
                     /locus_tag="VFZ86_04640"
     CDS             3069..3728
                     /locus_tag="VFZ86_04640"
                     /inference="COORDINATES: protein motif:HMM:NF024764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5641612.1"
                     /translation="MLLDRRKVKFWQKIVFGGMAFLMAAFLVVGYSGVLNGCTWFNSS
                     QQDVTEELDQQIAKYQAAVDADPTDVAAWKNLAQQLEFRANQEQQGSPAQRAFWLRAA
                     DAYTKADDLLAEQKGKEAREQRLEVLRRTADIQLFLQDYQAATSIYGKITALKPKDPQ
                     SYFEMASVAINAGDTSTALLAFTKFLELDPQSPDAPQVKAWIEANTPGSTPKPSPSPT
                     N"
     gene            3741..4091
                     /locus_tag="VFZ86_04645"
     CDS             3741..4091
                     /locus_tag="VFZ86_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018727574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX5641613.1"
                     /translation="MSDMFSVSSERLDDDLGVIVLSGEVDIYTAPQFKECLLELLDSG
                     VKRLVVDLSQITFIDSTALGVLIGGVRRVHGSGGAMTIVVTTRPVERVLSITGLDRVF
                     SMHATRDEALAALA"
     gene            4188..6455
                     /locus_tag="VFZ86_04650"
     CDS             4188..6455
                     /locus_tag="VFZ86_04650"
                     /inference="COORDINATES: protein motif:HMM:NF005555.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase I"
                     /protein_id="HEX5641614.1"
                     /translation="MKLIVTEKDSAARKIAEILGGKVTVQEHGRGRQKVRSYHFTHDG
                     GDAVAIGLRGHVMETVFPNTYKRWSLKYLGDMIEKPGLAWVVDGGAAGTLAALRAAAK
                     GATELVIATDYDREGELIGHEALEILRGDALKHNPGDKPEKKKRKAAAAKDGEARDKT
                     PAPAPSNGDPHVKPMLPAAVVDRHVRVRYSALTPEEVNAAFAKPSTVDFNLAEAAHSR
                     QDIDLIWGAVLTRFMSLASYRYGSDYLSVGRVQSPTLRLVVDRELERRAFVAVPYWEI
                     KATLEHDGAPFEVAHASGRFDTQEAMEKALERAQVETAEVTAYKAEPRQDKPPAPFNT
                     TALMSAASGAGMTPARAMRAAESLYLDGLISYPRTDNTVYPPSLDLHGSLRELARWQP
                     VAATAARLAGQDKLTPSRGKKRTTDHPPIYPVGVPKKELSGDQAKVYDLVARRFLATL
                     LPAAVIERQRVDIALGPEPFLAYGARVASLGYLEVYERYAAKRDRPLPPLQPGDTLKV
                     LELRSEAKETQPPGRYGQGKLLEKMEELGLGTKATRADIIQHLYDRNYVRDNPVEPTE
                     LGMALIAAFDAAMEKAPVDISSSQMTARLEKEMDRISEGALRREDVVAESQEMLEQAW
                     KLLDAHADDIRDRIKSGVREDLTLGTCKNCGGQLRVLRGKTGKRFAACVGKEGEEPQQ
                     PAAEGERPRRGCGQTFPLPQRGTIVPTGKVCAECGWPEIKVVGGGGRGRPWVLCIDID
                     CPSKEKYKARKKAAG"
     gene            6486..7175
                     /gene="tmk"
                     /locus_tag="VFZ86_04655"
     CDS             6486..7175
                     /gene="tmk"
                     /locus_tag="VFZ86_04655"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012946830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEX5641615.1"
                     /translation="MPERGSSGKRRGVLITLEGCDGCGKSTQAALLCERLAACGLPAG
                     PASAPGTVIREPGATPAGEAIRDVLLHGPSSLAPWTEALLYAAARAELVETVLLPELA
                     AGRVLILDRFVDSSLAYQGFARRLGVDEVLAVNQPGLRGLLPDLTLLLDVDPVVGLAR
                     AGGAGSADRIEAEGLEFQQRVAAGFAALARRWPERVRLIDGFRDVELVAADVEAAALE
                     AVADAGLRLGD"
     gene            7186..8319
                     /locus_tag="VFZ86_04660"
     CDS             7186..8319
                     /locus_tag="VFZ86_04660"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit"
                     /protein_id="HEX5641616.1"
                     /translation="MFADIPGQEQAKAFFERAAGSGALSHAYLLAGPEGLAKTEFARE
                     LGVALVAPCEGCGACPECARARRGMHPDLHLLEREGDVIRVEQIEPVVADLSLKPFSA
                     SRRVWVIPEVEYLHPAAANKLLKSIEEPPSFVVFILVTDRLERVLPTIVSRCQVVEFR
                     PLSDTEVSAYLVERHHVDPGAAEALARLSLGSVERAARFAEDAAGPRRREDYLRYAAA
                     LTAGERPADGPEPGAAFIAVLERQQAEIAAALEEDLARRLADLERQFQDKKDLEWYVK
                     QAESRAKREDARRRRLAAQDALDLLVSWVRDLWVVASGASDVLWNSDHRDALVEAVVA
                     TPEHYARLLGVSAKTRKDLYLNIDQKLALQAMFARFEEVSASA"
     gene            8312..9130
                     /gene="ricT"
                     /locus_tag="VFZ86_04665"
     CDS             8312..9130
                     /gene="ricT"
                     /locus_tag="VFZ86_04665"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HEX5641617.1"
                     /translation="MPRICSVVFRGGGRVYQFSAGDLELAPGDGVVVDTTRGTDFGRV
                     VKAAAEVAAADAPRGLRRVLRKATPADLEAIASRRETECSAKRECRRLAGELKLDIKV
                     VEARQAFDGPRLTITFFAEERTDVRELQNRLGDRVGRRVELKQVSARDESRIVGGYGP
                     CGRSLCCATFAGDQEPVSIRMAKDQNLPLNPTKISGCCGRLMCCLKYEHQVYVSFRKR
                     APKRGAIVRTPAGEGKVTDLLAPVDSVTVDLGEGRSATFKLDELGSAAAPGRND"
     gene            9203..9436
                     /locus_tag="VFZ86_04670"
     CDS             9203..9436
                     /locus_tag="VFZ86_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014143477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper ion binding protein"
                     /protein_id="HEX5641618.1"
                     /translation="MAETHVVLNVPSVSCNHCKMAIEKAVGALGGVGAVDVDVAEKTV
                     TVDFDADAVTLEAIEAACAEEGYEVAGRHVFGA"
     gene            9493..9568
                     /locus_tag="VFZ86_04675"
     tRNA            9493..9568
                     /locus_tag="VFZ86_04675"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9526..9528,aa:Lys,seq:ctt)
     gene            9747..10541
                     /locus_tag="VFZ86_04680"
     CDS             9747..10541
                     /locus_tag="VFZ86_04680"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX5641619.1"
                     /translation="MNARLRPTVGRLLNDARFSFAPSGGEDVVRQVLIMVVCYAAYDV
                     SRALAQGRDAIALANGVFFMNLEKALGIWWEPWIQGRVSTVEPLMTALVWIYQYAHLP
                     IIIGAMVWVFTQHRGVWVLYRNWFLVMNFIAVIVFALLPTAPPRMIFTSGVVDINFLH
                     GVRTSILENGLLANPFAAMPSLHFGYALFIGIMLYTLARGRWTRYLGFLYAVVVLVAI
                     VATGNHFIIDAVAGALVALAAWAFAVNVQPSAVPQSPAVTVRERDG"
     gene            10543..11262
                     /locus_tag="VFZ86_04685"
     CDS             10543..11262
                     /locus_tag="VFZ86_04685"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX5641620.1"
                     /translation="MTNLKTRYTAGARAGFFRLGAILARRGFTADMMTYIGLLLQVAA
                     LPLIITGYFGWALVVGIIASLCDGMDGAVARAGAGPTKAGAFLDSTLDRVSELMVAAA
                     LVVYFVREGPEWAPIIAVLVFMGAAQMVSYIRARAEALGVDCKVGFMSRPERLVGLGI
                     AFLFSWWEPGGTSFLVWWLYFLTLLTVITVVHREVHVLRKLRALEGPPALEEPPAAAP
                     AAEPLADDLWPEPSGRGDRAY"
     gene            11419..12522
                     /locus_tag="VFZ86_04690"
     CDS             11419..12522
                     /locus_tag="VFZ86_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011309420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HEX5641621.1"
                     /translation="MGNPVRVAIIGVGNCASSFVQGVEFYKNAKKSDFVPGLMHPVVG
                     GYHVRDIEFVAAIDIDKNKVGKDLSEAVFTTPNNTVKFSDVPNKGVTVARGMTHDGLG
                     KYLSQIIEKAPGPTADIVGILKETQTDVVVSYLPVGSEEATKWYAEQILTAGCAMVNC
                     VPVFIAREPYWQKRFVEAGVPIIGDDIKSQVGATIVHRVLTRLMRERGVKLERTSQLN
                     VGGNTDFLNMLERERLESKKISKTNAVTSQLDYDMGADNVHIGPSDYVKWLDDRKWAY
                     IRMEGRTFGDVPLNIELKLEVVDSPNSAGIVIDAVRCAKIGLDRGLAGSLEGPSSYFM
                     KSPPVQHPDDQCRRMTEEFISGDGPGAGAVCQG"
     gene            12623..14518
                     /locus_tag="VFZ86_04695"
     CDS             12623..14518
                     /locus_tag="VFZ86_04695"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5641622.1"
                     /translation="MALKLGILTPFLWSTPSAVNEQVRALAERLTRAGHRVTVIAPTA
                     DRPAVAEARRRVQAVLTGDRETVFLADEPYPHFFFAGATYAVKESGSLKLIAAPADLI
                     ANIDVLLGAEDFDLLHLNEPFVPGLGWSALRHAGCPLVATFHANPEKMRPFWSTRPQL
                     RRLFDSLDAAIASSAAARDAAALTFPGAYRVIPPGVDHALFRPAGERPAGPRRVVYSA
                     GVERRKGLGVLLRALRHLGDGHDDVVVEVCGGDLQERRYARLVPPAWQDRVRFHGRLS
                     RPEVASLLRAADVFCAPSFGPETAGVALIEAMASGATVVASQIPGYDEVVQNEGTGLL
                     VPPRDPAALATALSRVLGEDELRHRLAAHALRASRRYDWDRVTGEILEVYGEAARRRR
                     DPLPRRRRQQRELFADFHIHTHHSKDCVMPVADILERAREVGLDVVAITDHDSVAGGI
                     EARELADRYGVRVIVGEEVKSSEGEVIGLFLERTIPGGMTFAESIAAIREQHGIVYVP
                     HPFDRLHTIPGPGVLRANVDDIDVVEVFNSRLAFPGFNELAERFAQRYRLPAAAGSDA
                     HVLPGIGTTLCGIDDFTGPDDFVAALAESRIVRRPRSRIYLQSLKLLQTTMGGAARND
                     GQVDSPA"
     gene            14623..15225
                     /locus_tag="VFZ86_04700"
     CDS             14623..15225
                     /locus_tag="VFZ86_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEX5641623.1"
                     /translation="MAQPNEIYDKYLTRAISEINELTGEILRCSRCSHARTMPVIGSG
                     HPMADIFLLKYQARPSELHEGVAFFGRAGTALMKSCQRLKIDPLQLYGTNVIKCGEVR
                     EAHPEAKCLDHLRRELAIVNPKLIVAMGEETVAALNTAKVPLARELEYAPGEIVDFTP
                     GTEVLVTPDIDVSLDEQRLKAGFWRAFRRLGEWYDNIPPY"
     gene            15254..15970
                     /locus_tag="VFZ86_04705"
     CDS             15254..15970
                     /locus_tag="VFZ86_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641624.1"
                     /translation="MKRLVVPALAVAVLLAWGFGSRLLPAPPLRANILLSSTIVLSAV
                     AVLVWGMLPLGALGRRLPLVALAALPLAVLFVWLGWVPAANVAKVVFAAALGIWIAGE
                     LERVSWIVVVAAVSAIVDVVSVAAGPTRAILDKGPVVVGYFIVAATWSGYSYDEAFTG
                     LGTSDVLFFALYLGAARRFRLRTGWSAVAMVASFLCTIALAMWWTALPALPLLSVAFL
                     AVNADLIVRGMRRGGEAPPA"
     gene            complement(16020..17408)
                     /gene="rlmD"
                     /locus_tag="VFZ86_04710"
     CDS             complement(16020..17408)
                     /gene="rlmD"
                     /locus_tag="VFZ86_04710"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEX5641625.1"
                     /translation="MPPRPGDIVELDVATLAYGGQGVARLDEFVVFVRGAVPGDRVRA
                     RVTRRKQSHAEARTLEVLSPSPRRVAARCRHAQECGGCEWQTLSYDAQLEFKQQQVVD
                     SLQRLAHLTEYRLEPIRGMDDPWRYRNKMEFSFGEDEHGGLVLGLHKRGSWREIVDIV
                     DCELASQRMNRARLAVAAACRHLGLRPYSRDGDGGLLRHLVVREGLASGDLLLNLFVA
                     ARFPEEAELAARVTAASGCTSFALTVNASRADAAVGDGPHMLSGPPSLRERLAGVDLR
                     VPAAAFLQTNSTMCETLYAIALRFAAPEPGRAAVDLYCGIGSLSLPLARAAKEVHAIE
                     IQGEAIDAARVNAELNRVDNAVFYAADVRPLLRFPPHPVLDAGRADEADRPAVVIADP
                     PRAGLARKALQRAAALGADRFAYVSCNPTTLAGNAAELGELGYRLTGVAPVDMFPHTH
                     HIETVALFERIA"
     gene            17593..19092
                     /locus_tag="VFZ86_04715"
     CDS             17593..19092
                     /locus_tag="VFZ86_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641626.1"
                     /translation="MSDLDLEQYRRRAEEFVGALDLEYYEHFSGRKPRCDTAAVYDRY
                     PELFTREAIDGLEELYAVAVDDEKLRLAYLLAFTVDGFMGEQTKHLGDEIANTEGEAT
                     ITVDGEVIGLRQASVAQGNEPDRARRARIQEARLAATAERLNPLHDTLWRRCHDLALE
                     LGYPDYKELYGTVRGLDYDHLRAELEGFLADTEGLYERVMDRLVRERLGVTLSELAFA
                     DLPFLWRAEGFDPVFRADALVPSLRRTLAGLGIDLDAQPNVHLDTEVRELKSPRAFCA
                     PVRVPDEIYLVVLPHGGQDDYGMLLHEAGHTEHFAHVRPDLAFEYRHLGDNAVTEGFA
                     FTFDHLILNRSWLADILDFEASDDYVRFAAINDLYFMRRYAAKLAYETTLHAQTGSLE
                     AMPAEYSRRLTSATKVKVPEENYLADVDDGFYCASYLRAWMLEGALRLMLQENFGMDW
                     YASRDAGDWLKALWAEGQHLTAERLLLRHGGGRLNTDPLRHLFEQVLGR"
     gene            complement(19096..19485)
                     /locus_tag="VFZ86_04720"
     CDS             complement(19096..19485)
                     /locus_tag="VFZ86_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HEX5641627.1"
                     /translation="MWTLEVQTRQRVSFIDITRDVARLVADAGDGTVVVFVPHTTAGV
                     TINEDADPSVRADLEMALERLVPRDLPFTHAEGNSDAHTKATLTGSSVTIPVVAGTPQ
                     LGTWQGIYFAEFDGPRRRRVLVTFLRA"
     gene            complement(19494..20147)
                     /locus_tag="VFZ86_04725"
     CDS             complement(19494..20147)
                     /locus_tag="VFZ86_04725"
                     /inference="COORDINATES: protein motif:HMM:NF024660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP-associated domain-containing protein"
                     /protein_id="HEX5641628.1"
                     /translation="MNVDLHTHTYPASDCSRISYRDYIAWCVDNHVEAIALTNHGDLS
                     DNRRLGPALAAEGVLLIDGVEISTLFGDFVVFSPDLDYLETLRSVQEAPRPGDVPDHA
                     AVVWVHPGAGGGRSGSAYYPGIENMVAGVIDAVEVYNGSWLDARYVGIAEQIADALGV
                     ARTGGSDAHDPAQLMVCSTEIPDPVTSTADVVRALRQGATVPQRRRASAARKRFRLF"
     gene            20176..>21001
                     /locus_tag="VFZ86_04730"
     CDS             20176..>21001
                     /locus_tag="VFZ86_04730"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HEX5641629.1"
                     /translation="MKGTLDTFGVVEILQILGRMRRSGTLHIECPLRLVDVHFTQGRI
                     AETRDSTRVAADTVIGSQLLKRSLVNDQQLAAALAEQEVRPRPIGTILVEHGAVAEDA
                     LREVLSRQIANTLVAAKLEESGSFVFVVDPEPQPVAYITVDTHSVLLDISALGGEYCL
                     AVEMLGQPSTVLVRNGDYNTLPRNPLLMGRDEFAVLLQVDGTRTVKEITQASRLEEIT
                     VVSILGKLADAGVLLVKAERHSRAEDAAELQAHRDSVWAEVSHLLDDMVEEPDAGAP"
BASE COUNT         3061 a         7560 c         7369 g         3011 t
ORIGIN      
        1 gtcaccttcc ggacacgctg gcgcaggagc tcctcgcgcg cctcccggac aagcgcgagc
       61 aggacctgcg ccgcctcgcc agccaccccg agcacacggc ggggtcgctc atgaccaccg
      121 acttcgtgat gctgccgcgc aaggccacgg ccggcaaggc gctcgcctgg atccgccggg
      181 aacgccccga gcagcacatg atgacgtatc tctacgtcgt cgacgccgag gagcggctcg
      241 tcggcgtggt gagcctgcgc gacctcgtcc tcgccgctcc ggacgatcag atgacgacgt
      301 tcatggagga cgatctcgtg gaggtgacgg ccgacgtgga cgaggaggag gtcggccgga
      361 tcatgaccaa gtacgacctc ctggccatcc ccgtggtcga cgcggagcgg cggctgctgg
      421 ggatcgtcac gctcgacgac gcgctggagg cggtcgcccc cgacgactgg aagcagcgcc
      481 tgccgcggct gtaccggtag gccggccgcg accgcagtcg cgcggccgtc agacgggctg
      541 cgtgcccgcc ggcgcctgca cgtgctccca cgcgacgctg cggcgcggcg cctcgatgag
      601 cgcggcgagg tcgagacgga acctggccac ctcgcccttc tgctgcaccg gtctgaaccc
      661 gccgccctcg agcacgtccg ggttgaagag attgagctcc cgcaggtagc cgcggaaggc
      721 gtcgtcgccg cgggcgtggc ccaggccgaa cgcctccagc gcagcgacgc ggcggtgctt
      781 gagcgccgct tcgatctcct ggagcaggaa gcggaagccg tgcaggtact cctcgtcgta
      841 gaagtaggcg caggtgagca ggtaggcgtc gggagaggcc gggcccgccg gcagggcgcg
      901 agcgtgtggg accagacgag ccgcggcgac gtgcatccag ccgatgaccg cgtcgccctc
      961 gagcagcgcc cgcccgaaga acccggcctc ggcctcggcc tcgcgctccc actcctgccg
     1021 cagcccgggc ccggccgtga tgccgggccg ggtgagccac cacgtgcagg tggagcagca
     1081 gggggccagc aggtcgctgc tggcgacggt caggtcgatc gccctcacgt ggaggggtcg
     1141 gcgatgtcgt cgacgtacac ctcgacgcgg gtgatcgggt tgcccgtcgt gcgcgtgaga
     1201 taggtggcgc cggcgcgggc ggccgcctcg ccgacggcgg ccaggtccga gccggcctcc
     1261 acgaccagat ggatctccag cagcgggccg tcctctccgt cgtccagccg cacggggccg
     1321 tggcgctcca ggtggactcg ctcgcccagg ctctgcaggg ggttgcgata gaggtcgcgg
     1381 acgcccgcga ggcccttcac cgcgtcccac accgcactgg tgacgacgtc cgctgagatg
     1441 ctcggtctct gtccctgctg cacgctgcgg ccctcccgtc gctggtgcgg ggagtatacc
     1501 gccgggacct gcccgtcgtg agtgacggcg ctgtttgcca ccgtgctcgg ggctccccta
     1561 tactcctgcc acgtgggggg ccgaagcgcc tccctcgcgc tctgggcagc cgcatgaccg
     1621 acaccgaccg cgacatcacg atctatcgtg ccgactgggt actcccggtg gccgcgcccg
     1681 tcctgcccga cgggggcgtc gccgtgggcg gcgatgggcg catcgtcgcc gtcggccccg
     1741 ctccgcgcct gatcgccgac cacccgggcg cggcggtcgt cgacaccggc cggtcgatca
     1801 tcatgccggg cttcgtcaat tgccacagcc acatggagta caccacgttc cgcggcatcc
     1861 tcgacgacgc cgagttcggc gactggatca tcagcctcgt ggacgtcaag gcgtcgctcg
     1921 cgccggacga gtaccgcatc agcgcccagc tgggcgccat ggaggctctc tcctccggca
     1981 tcacgacggt cgccgacacg agttacggcg cggcgacgct ggaggcggcc ggtgcggccg
     2041 gactccgtgg ccgcctgtac ctcgaggtct tcggcgtcga cgacgcgcgc ttcgacgaga
     2101 ccatggcgga cgtggtgcgg cggctcgacg ccgcgcaggc cgccgcgccg gccacgttcg
     2161 aggtcggcct ggcgccgcac gctccctaca cggtctccag ccggctgtac caggccgtga
     2221 gcgcgctggc ccgcgagcgc ggcctgaagg tcatgagcca cgtcgccgag agccaggacg
     2281 agctcaccta catccgctcc ggctcgggca agttcgcgca cgactaccgc gagaagatgg
     2341 gctgggagcg catgctcgtc cagccctacg gcgtcacgcc catcaagtac ctgcagcagt
     2401 gggacgtgct gcacccggac ttcctcgccg tccactgcgt caacgtgacg cgcgacgaca
     2461 tccgcatcct gcgcgccaaa gacgccgccg tggcgcactg ccccaagagc aacgccaagc
     2521 tcggctgcgg cgtcgccccg ctcgccggcc tcctgcacga gggggtgcgt gtcggcctcg
     2581 gcacggacag cccggcctcg agcaacatca tggacatgtt cgacgagatg cggacgatgc
     2641 tgttcctgca ccgcgcggtc gagcgcgacg tgggcgtgct cgacgcccag cggtgcgtgc
     2701 gcatcgccac gctggggggc gcggaggcgc tgggcatgga ggccgccgtc ggcagcctcg
     2761 agcccggcaa gtgggccgac ctcatcgccg tcgacgtctc gcgctcgcac ttcgcgccga
     2821 tcgccgatcc gtactcggcg ctcgtctacg gcgccaacca ggacgacgtc acgctcacgg
     2881 tcgtcggcgg acgtgagctg taccgcgacc ggatccacgt ggacgtcgat gccgacgcca
     2941 tccgggcccg ggccgtcgcc gtccgcgaga agcttcagga ccgcgtgctc accggcgccg
     3001 tcgaggtcgg cggcgccgac tccggctggt ggcacgtggc gcccgtccac cgagaggaga
     3061 gcgtctaagt gctgctcgat cgtcgcaagg tcaagttctg gcagaagatc gtcttcgggg
     3121 ggatggcctt cctcatggcc gccttcctcg tcgtagggta ctcgggagtg ctcaacggct
     3181 gcacgtggtt caacagcagc cagcaggacg tgaccgagga gctggaccag cagatcgcca
     3241 agtaccaggc cgcggtcgac gcagacccca cggatgtggc ggcctggaag aacctggccc
     3301 agcagctcga gttccgcgcc aaccaggaac agcagggctc gccggcccag agggccttct
     3361 ggctgcgggc ggcggacgcc tataccaagg ccgacgatct gctcgccgag cagaagggca
     3421 aggaagcgag ggagcagcgc ctggaggtgc tccggcgcac cgccgacatc cagctcttcc
     3481 tgcaggacta ccaggcggcc accagcatct acggcaagat caccgcgctc aagcccaagg
     3541 acccgcagtc gtacttcgag atggccagcg tcgccatcaa cgcgggggac accagcaccg
     3601 cgctgctcgc cttcaccaag ttcctggagc tcgacccgca gtccccggac gcgccgcagg
     3661 tcaaggcctg gatcgaggcc aacacgccgg gctccacgcc caagccatcg ccatcgccga
     3721 ccaactaagg ggtccggtcc atgagtgata tgttcagtgt ctccagcgag cgtctcgacg
     3781 acgatctcgg ggtgatcgtc ctcagcggcg aggtcgacat ctacaccgcc ccgcagttca
     3841 aggagtgcct cctcgagctg ctggactccg gcgtgaagcg gctcgtggtc gacctcagcc
     3901 agatcacgtt catcgattcg accgctctcg gcgtcctcat cggcggggtg cgccgcgtgc
     3961 acggctccgg cggcgccatg accatcgtcg tcaccacgcg gccggtcgag cgcgtgctca
     4021 gcatcaccgg cctcgaccgc gtgttctcga tgcacgccac gcgtgacgag gctctcgccg
     4081 cgctcgcctg accggcagcg ccaacgtcaa tctcgactgc cgggtttgcc gggtggtatt
     4141 aactccggca gtcttggtcg tctctctcta ccgtcattga ggtcacagtg aagctcatcg
     4201 tcacagaaaa ggactcggcc gcgcgcaaga tcgcggagat cctgggcggc aaggtgaccg
     4261 tccaggagca cggtcgcggc cgccagaagg tcaggtcgta ccacttcacc cacgacggcg
     4321 gagacgccgt ggccatcggg ctgcgcgggc acgtcatgga gaccgtgttc cccaacacct
     4381 acaagcgctg gagcctcaag tacctcggcg acatgatcga gaagcccggc ctcgcctggg
     4441 tggtggacgg cggcgccgcc ggcacgctgg ccgcgctgcg cgccgccgcc aagggcgcca
     4501 ccgaactggt catcgccacc gactacgacc gtgagggcga gctcatcggc cacgaggcgc
     4561 tcgagatcct gcgcggcgac gcgctcaagc acaaccccgg cgacaagccg gagaagaaga
     4621 agcgcaaggc ggccgccgcc aaggatgggg aggcgcgcga caagacgccg gcgcccgcgc
     4681 cctcgaacgg cgacccgcac gtcaagccca tgttgccggc ggccgtggtg gaccggcacg
     4741 tgcgcgtgcg ctactcggcg ctcacgccgg aggaggtcaa cgccgccttc gccaagccct
     4801 ccaccgtgga cttcaacctc gccgaggcgg cgcactcccg ccaggacatc gacctcatct
     4861 ggggcgcggt gctcacgcgc ttcatgtcgc tggcctccta ccggtacggc tcggactacc
     4921 tctcggtggg ccgtgtgcag tcgccgaccc tgcgtctcgt ggtcgaccgc gagctcgagc
     4981 ggcgcgcctt cgtcgccgta ccctactggg agatcaaggc gacgctcgag cacgacggcg
     5041 ccccgttcga ggtggcgcac gcgagcgggc gcttcgacac gcaggaggcg atggagaagg
     5101 cgctcgagcg cgcccaggtc gagaccgcag aggtcacggc gtacaaggcc gaaccgcgcc
     5161 aggacaagcc gccggcgccg ttcaacacca ccgcactcat gagcgcggcg tcgggcgccg
     5221 gcatgacgcc ggcgcgcgcc atgcgcgccg ccgagtcgct gtacctcgac ggcctcatca
     5281 gctacccgcg cacggacaac accgtctatc cgccgtcgct cgatctgcac ggcagcctgc
     5341 gcgagctggc gcgctggcag ccggtcgccg ccacggcggc ccggctcgcc ggtcaggaca
     5401 agctcacgcc gtcgaggggc aagaagcgca ccaccgacca tccgccgatc tacccggtcg
     5461 gcgtgcccaa gaaggagctg agcggcgacc aggccaaggt ctacgacctg gtcgcccggc
     5521 gcttcctggc gacgctgctg ccggcggcgg tcatcgagcg gcagcgcgtc gacatcgccc
     5581 tgggcccgga gccgttcctc gcctatggcg cccgcgtggc gagcctcggc tacctcgagg
     5641 tctacgagag gtacgcggcc aagcgcgacc gcccgctgcc gccgctgcag ccgggcgaca
     5701 cgctcaaggt gctcgaactg cgctcggagg ccaaggagac gcagcctccg ggccgctacg
     5761 gccagggcaa gctgctcgag aagatggagg agctcggcct gggcaccaag gccacgcgcg
     5821 ccgacatcat ccagcacctc tacgaccgca actacgtgcg cgacaacccc gtggagccca
     5881 ccgagctcgg gatggcgctc atcgccgcgt tcgacgccgc catggagaag gcgccggtcg
     5941 acatctcgtc cagccagatg accgccaggc tggagaagga gatggaccgc atcagcgagg
     6001 gcgcgcttcg ccgcgaggac gtcgtcgccg agagccagga gatgctcgag caggcctgga
     6061 agctgctcga cgcgcacgcg gacgacatcc gtgaccgcat caagagcggg gtgcgcgagg
     6121 acctcacgct cggcacctgc aagaactgtg gcgggcagct ccgcgtgctc cgcggcaaga
     6181 cgggcaagcg cttcgccgcc tgcgtcggca aggagggcga ggagccgcag cagcccgccg
     6241 ccgagggcga gcgcccgcgg cgcggctgcg ggcagacgtt cccgctgccg caacgcggca
     6301 ccatcgtgcc gaccggcaag gtctgcgcgg agtgcggctg gccggagatc aaggtggtcg
     6361 gcggcggcgg gcgcgggcgc ccgtgggtgc tctgcatcga catcgactgc ccgagcaaag
     6421 agaagtacaa ggcgcggaag aaagccgccg gctgagggcc ggccgggccg gcgggggaga
     6481 gactcatgcc tgagcgaggc tcgtccggga agaggcgcgg cgtcctcatc acactcgagg
     6541 gatgcgacgg gtgcggcaag agcacccagg cggccctgct gtgcgagcgt ctggccgcct
     6601 gcggtctgcc ggcagggccg gcgtccgcgc cgggcacggt catcagggag cccggcgcca
     6661 cccccgccgg ggaggccatc cgcgacgtgc tgctccacgg cccctcctcg ctggcgccgt
     6721 ggaccgaggc cttgctctac gcagccgcgc gcgccgagct cgtcgagacc gtcctgctgc
     6781 ccgagctcgc ggcgggccgc gtgctcatcc tcgaccgctt cgtcgactcg tcgctcgcct
     6841 atcagggctt cgcccgccgg ctgggcgtcg acgaggtcct cgccgtcaac cagccggggc
     6901 tgcgcggcct gctgccggac ctcaccctgc tgctcgacgt cgaccccgtg gtcgggctgg
     6961 cgcgcgccgg cggcgccggc tccgccgacc ggatcgaggc ggaggggctc gagttccagc
     7021 agcgcgtggc ggccggcttc gccgcgctcg cccgtcgctg gcccgagcgg gtccggctca
     7081 tcgacggttt tcgcgacgtc gagctcgtgg ccgccgacgt ggaggccgcc gcgctcgagg
     7141 ccgtcgccga cgccggcctg cggctcggcg actgaggagc cggtcgtgtt cgccgacatc
     7201 cccgggcagg agcaggccaa ggccttcttc gagcgtgccg ccgggtccgg cgcgctgagc
     7261 cacgcctacc tgctggccgg cccggaaggc ctggccaaga cggagttcgc gcgggagctc
     7321 ggcgtggccc tcgtcgcgcc ctgcgagggc tgcggcgcct gccccgagtg cgcgcgcgcc
     7381 cgccgcggca tgcacccgga cctgcacctg ctcgagcgcg agggcgacgt gatccgcgtg
     7441 gagcagatcg agccggtcgt cgccgacctc agtctcaagc cgttctcggc ctcccgccgc
     7501 gtgtgggtca tccccgaggt ggagtacctg cacccggcgg ccgccaacaa gctgctcaag
     7561 agcatcgagg agccgccgtc gttcgtcgtc ttcatcctgg tcacggacag gctggagcgc
     7621 gtcctgccga ccatcgtctc gcgctgccag gtggtcgagt tccggccgct cagcgacacc
     7681 gaggtgagcg cgtacctcgt ggagcggcac cacgtcgacc cgggcgccgc cgaggcgctg
     7741 gcgcggctgt cgctcggctc ggtcgagagg gcggctcgct tcgcggaaga cgcggcggga
     7801 ccacggcgcc gcgaggacta cctgcggtac gcggccgcgc tgacggccgg cgagcgcccc
     7861 gccgacgggc ccgagccggg cgctgcgttc atcgccgtcc tcgagcggca gcaggcggag
     7921 atcgccgccg cgctcgagga ggacctcgcg cggcggctgg cggacctcga gcggcagttc
     7981 caggacaaga aggacctcga gtggtacgtc aagcaggcgg agagccgggc aaagcgcgag
     8041 gacgcgcgcc gccgccggct cgccgcccag gacgcgctgg accttctcgt gtcgtgggtg
     8101 cgcgacctct gggtggtcgc ttccggggca tccgatgtac tctggaacag cgaccaccgc
     8161 gacgcgctcg tcgaggcggt cgtggcgacg ccggagcact acgcgcgtct cctcggtgtc
     8221 agcgccaaga cccgcaaaga cctgtatctc aacatcgacc agaagctcgc gctgcaagcg
     8281 atgttcgcgc gcttcgagga ggtctccgcc agtgcctagg atctgcagcg tcgtgttccg
     8341 cggcggcggc agggtctacc agttcagcgc cggcgacctc gagctggcgc ccggcgacgg
     8401 cgtcgtggtc gacaccaccc ggggcacgga cttcgggcgg gtcgtcaagg ccgcggccga
     8461 ggtggccgcc gccgacgccc cgcgcggcct ccgccgggtg ctgcgcaagg cgacgcccgc
     8521 cgacctcgag gcgatcgcct cgcgccgtga gacggagtgc tcggccaagc gcgagtgccg
     8581 gcgcctcgcg ggcgagctca agctcgacat caaggtcgtc gaggcacgcc aggcgttcga
     8641 cggccccagg ctcaccatca ccttcttcgc cgaggagcgc accgacgtgc gcgagctcca
     8701 gaaccggctc ggcgaccgtg tcggccgccg ggtcgagctc aagcaggtga gcgcccgcga
     8761 cgagtcgcgc atcgtcggag gctacggtcc gtgcggccgc agcctctgct gcgccacctt
     8821 cgccggcgac caggagccgg tgtccatccg catggccaag gaccagaacc tgccgctcaa
     8881 ccccaccaag atcagcgggt gctgcggccg gctcatgtgc tgcctcaagt acgagcacca
     8941 ggtctacgtc tcgttccgca agcgggcgcc caagcgcggc gcgatagtcc gcacgcctgc
     9001 cggggaaggt aaggtcacgg atctgctggc gccggtcgac agcgtcaccg tcgacctcgg
     9061 agagggccgg tcggccacct tcaagctcga cgagctcggg tcggccgccg cgccgggaag
     9121 gaacgactaa tgcccgtaga gtcacgaagc atacggagcg ctattgagcg actcgctggc
     9181 cgaaggcact gcgaggcgga acatggctga gacgcacgtc gtgctcaacg taccgtcggt
     9241 gtcctgcaac cactgcaaga tggcgatcga gaaggcggtc ggcgccctgg gcggcgtcgg
     9301 cgcggtcgac gtcgacgtcg ccgagaagac cgtgaccgtc gacttcgacg ccgacgccgt
     9361 gacgctcgaa gcgatcgaag cggcctgtgc agaagagggc tacgaggtcg ccggacgcca
     9421 cgtcttcggc gcctgacgga gccgtttggc aagatcgcgg tcggctggta ccatatcgcc
     9481 tcgcacgcgc gtgggccgtt agcttagttg gtagagcagg ggactcttaa tcccaaggtc
     9541 gaaggttcga atccttcacg gcccaccatc cccaccacgt tccgcgtccg gaccggcgcg
     9601 ccgcgagcgc cgcaacaccc cgcgcaggag gcggcgcgca gaacgggaac actctgtctc
     9661 agttgcccgg gcgttcgggg ctctgttatc gttcttcgga tacgggcgcc cgcccgtgac
     9721 gtccacctga cgggaggtcg aagagggtga acgcgcgtct caggcccacc gtgggcaggc
     9781 tcctcaacga cgcgagattc tccttcgccc ccagcggcgg cgaggacgtc gtgcgccagg
     9841 tcctcatcat ggtcgtgtgc tacgccgcct acgacgtctc gcgcgcgctc gcgcagggcc
     9901 gcgacgccat cgcgctggcc aacggggtgt tcttcatgaa cctcgagaag gcgctgggca
     9961 tctggtggga accctggatc cagggccgag tgagcacggt ggagccgctg atgacggcgc
    10021 tggtgtggat ctaccagtac gcgcaccttc ccatcatcat cggagccatg gtgtgggtgt
    10081 tcacgcagca ccggggcgtg tgggtgctgt accgcaactg gttcctcgtg atgaacttca
    10141 tcgcggtgat cgtgttcgcg ctgctgccca cggcgccgcc gcggatgatc ttcacgtcgg
    10201 gcgtcgtcga catcaacttc ctgcacggcg tgcgcacgtc gatcctcgag aacgggctgc
    10261 tcgcgaaccc gttcgccgcc atgccgagcc tgcacttcgg ctacgcgctc ttcatcggca
    10321 tcatgctcta cacgctcgcg cgcggccgct ggacccggta tctcggcttc ctctacgccg
    10381 tggtcgtgct ggtcgccatc gtggcgacgg gcaaccactt catcatcgac gccgtcgccg
    10441 gggcgctcgt cgccctggcc gcgtgggcct tcgccgtgaa cgtgcagccg agcgccgtgc
    10501 cgcagtcgcc ggcagtcacc gtgcgcgagc gcgacggctg acgtgacgaa cctcaagacg
    10561 cggtacaccg caggtgcccg ggccgggttc ttccgcttgg gcgcgatcct cgcgcgccgc
    10621 ggcttcaccg ccgacatgat gacctacatc ggcctgctgc tgcaggtcgc cgccctgccg
    10681 ctcatcatca ccgggtactt cggctgggcg ctcgtggtcg gcatcatcgc gtcgctctgc
    10741 gatggcatgg acggggccgt ggcgcgtgcc ggcgccggcc ccaccaaggc cggtgcgttc
    10801 ctcgactcca cgctcgaccg cgtctccgag ctcatggtgg ccgcggccct cgtggtctac
    10861 ttcgtgcgcg agggcccgga gtgggctccc atcatcgccg tcctcgtctt catgggcgcg
    10921 gcgcagatgg tctcgtacat ccgcgcccgg gcggaggcgc tcggcgtgga ctgcaaggtc
    10981 ggcttcatgt cgcggccgga gcgcctggtc ggcctcggca tcgcgttcct cttctcgtgg
    11041 tgggaaccgg gtggcacgag cttcctcgtg tggtggctgt acttcctcac actgctcacc
    11101 gtgatcaccg tggtgcaccg cgaggtccac gtgctgcgca agctgcgcgc cctggagggg
    11161 ccgccggcgc tcgaggagcc tcctgcggcg gcgcccgcgg ccgagccgct cgcggacgac
    11221 ctctggcccg agcccagcgg ccgcggggac cgcgcgtact gagccgtctc cctgtggtgg
    11281 ccgcgccggt ccggccgcct ccgcgtgata tactcccgcg cgttatctcg agccggtcgg
    11341 gcctgcctcg tgcgcccgga ccccgctccg atcgacgcgg ccgccggggt ggccgcatcc
    11401 agtggaggta gaccgatctt gggcaatcca gttcgcgttg cgatcatcgg cgtcggcaac
    11461 tgcgcttctt cgttcgtgca gggcgtggag ttctacaaga acgccaagaa gagcgacttc
    11521 gtcccgggcc tcatgcaccc cgtcgtgggc ggctaccacg tccgggacat cgagttcgtg
    11581 gccgccatcg acatcgacaa gaacaaggtg ggcaaggacc tcagcgaggc cgtcttcacc
    11641 actcccaaca acaccgtcaa gttcagcgac gtgcccaaca agggcgtcac cgtcgcccgt
    11701 ggcatgacgc acgacgggct cggcaagtac ctctcgcaga tcatcgagaa ggctcccggc
    11761 ccgaccgccg acatcgtcgg catcctcaag gagacgcaga ccgacgtggt ggtcagctac
    11821 cttcccgtcg gcagcgaaga ggccaccaag tggtacgccg agcagatcct caccgccggc
    11881 tgcgccatgg tgaactgcgt gcccgtgttc atcgcccgcg agccctactg gcagaagcgc
    11941 ttcgtcgagg ccggcgtgcc gatcatcggc gacgacatca agagccaggt cggcgccacc
    12001 atcgtgcacc gcgtgctcac ccgcctcatg cgcgagcgcg gcgtcaagct cgagcgcacc
    12061 agccagctca acgtgggcgg caacaccgac ttcctcaaca tgctcgagcg cgagcgcctc
    12121 gagtccaaga agatcagcaa gaccaacgcc gtcaccagcc agctcgacta cgacatgggc
    12181 gccgacaacg tgcacatcgg ccccagcgac tacgtcaagt ggctcgacga ccgcaagtgg
    12241 gcctacatcc gcatggaggg ccgcaccttc ggcgacgtgc ccctcaacat cgagctcaag
    12301 ctcgaggtcg tcgactcgcc gaacagcgcc ggcatcgtca tcgacgcggt gcgctgcgcc
    12361 aagatcgggc tggaccgcgg cctcgccggc tcgctcgagg gccccagcag ctacttcatg
    12421 aagtcgccgc cggtgcagca tcccgacgac cagtgccgcc gcatgaccga ggagttcatc
    12481 agcggcgacg gtccgggcgc cggcgccgtc tgccaggggt agtcaccgca agcgatccgc
    12541 tccgaagggg cggcgagggt atgagccctc gccgcccctt cgtgcgtgtg gtctcgcggc
    12601 ggcgtgcggt accatgacgg tcatggctct caagctcggc atcctcacgc cgtttctctg
    12661 gtccacgccg tcggccgtga acgagcaggt ccgggcgctg gccgagaggc tcacgcgggc
    12721 cgggcaccgg gtgacggtga tcgccccgac cgccgaccgc ccggcggtcg ccgaggcgcg
    12781 ccgccgcgtc caggcggtgc tcaccggcga ccgtgagacg gtgttcctgg ccgacgagcc
    12841 gtatccccac ttcttcttcg ccggcgccac gtacgcggtc aaggagagcg gttccctcaa
    12901 gctcatcgcg gcgccggcgg acctcatcgc caacatcgac gtcctgctgg gggccgagga
    12961 cttcgacctt cttcacctca acgagccgtt cgtccccggc ctgggctggt cggcgctgcg
    13021 ccacgcgggc tgcccgctgg tcgcgacgtt ccacgccaac ccggagaaga tgcggccgtt
    13081 ctggtccacc cggccgcagc ttcgccgcct cttcgactcg ctcgacgcgg cgatcgcctc
    13141 gtcggccgcc gcgcgcgacg cggcggcgct cacgtttccc ggcgcgtacc gcgtcatccc
    13201 gccgggcgtg gaccacgcgc tgttccgccc ggccggcgag cgccccgccg ggccgcggcg
    13261 cgtggtgtac agcgccggcg tcgagcggcg caaggggctc ggcgtgctcc tgcgcgctct
    13321 gcgtcacctc ggcgacgggc acgacgacgt cgtcgtcgag gtctgcggcg gggatctgca
    13381 ggaacggcgc tacgcgcgtc tggtgccgcc cgcctggcag gaccgcgtcc gcttccacgg
    13441 gcggctgagc cgcccggagg tggcctcgct gctgcgcgcg gccgacgtgt tctgcgcgcc
    13501 gtccttcggc cccgagacgg cgggcgtcgc cctgatcgag gccatggcct ccggcgccac
    13561 cgtggtcgcc tcgcagatcc ccggctacga cgaggtggtg cagaacgagg gcaccggcct
    13621 cctcgtcccg ccgcgcgacc ccgctgcgct cgcgacggcg ctctctcggg tgctcggcga
    13681 agacgagctc cgccatcggc tggcggccca cgccctgcgc gcttcccgcc gctacgactg
    13741 ggaccgggtg accggggaga tcctcgaggt gtacggggag gcggctcgcc ggcggcgaga
    13801 cccgctgccg cgccgccgtc gccagcagcg cgagctgttc gccgacttcc acatccacac
    13861 gcaccacagc aaggactgcg tgatgcccgt ggccgacatc ctcgagcggg cccgcgaggt
    13921 cggtctggac gtggtcgcga tcaccgacca cgacagcgtc gccggcggca tcgaggctcg
    13981 cgagctggcc gaccgctacg gcgtccgcgt catcgtcggg gaggaggtca agtcgagcga
    14041 gggcgaggtg atcggcctct tcctggagcg caccatcccc ggcggcatga ccttcgccga
    14101 gagcatcgcc gcgatcaggg agcagcacgg catcgtctac gtgccgcacc ccttcgaccg
    14161 gctgcacacg atccccggtc ccggggtcct gcgcgccaac gtcgacgaca tcgacgtcgt
    14221 cgaggtcttc aactcgcggt tggcgttccc ggggttcaac gagctcgccg agcgcttcgc
    14281 gcagcggtac cggctgccgg ccgcggccgg cagcgacgcg cacgtgctgc cgggcatcgg
    14341 gaccacgctc tgcggcatcg acgacttcac cggaccggac gacttcgtgg cggccctcgc
    14401 cgagagccgt atcgtccgcc ggccgcggag ccgcatctac ctgcagtcgc tcaagctgct
    14461 gcagaccacg atgggtgggg ccgcgagaaa cgacgggcag gtggattccc ctgcatagcg
    14521 cgcggataat cgaggcgttc ccgcatttgt cgggccggtg cgaggagttc gctgcgtggc
    14581 ggcgaactcg ctagccagca gcacccctga gagaggtacc gcgtggcaca acccaacgag
    14641 atctacgaca agtacctgac ccgggccatc agcgagatca acgagctgac cggcgagatc
    14701 ctcaggtgtt cgcgctgcag ccacgcgcgt accatgccgg tgatcggctc ggggcacccg
    14761 atggcggaca tcttcctgct caagtaccag gcgcggccgt ccgagctgca cgaaggcgtc
    14821 gccttcttcg gccgggccgg aaccgcgctc atgaagagct gccagcgcct caagatcgat
    14881 ccgctgcagc tgtacggcac caacgtcatc aagtgcggcg aggtccgcga ggcgcatccg
    14941 gaggccaagt gcctcgacca cctgcgccgc gagctcgcga tcgtcaatcc caagctcatc
    15001 gtcgccatgg gcgaggagac agtcgccgcg ctcaacaccg ccaaggtgcc cctggcccgc
    15061 gagctcgagt acgcgcccgg cgagatcgtc gacttcacgc cgggcaccga ggtgctcgtc
    15121 acgcccgaca tcgacgtgtc gctggacgag cagcggctca aggccgggtt ctggcgggcc
    15181 ttccggcgcc tcggcgagtg gtacgacaac atcccgccgt attgatgtcg gcggctccgg
    15241 ccgcccgccc cggatgaaac gcctcgtcgt tcccgccctc gcggtcgcgg tgctgctcgc
    15301 ctggggcttc ggcagccgcc tcctgccggc gcctccgctg cgcgcgaaca tcctgctgtc
    15361 ctcgacgatc gtgctctccg ccgtcgcggt gctcgtgtgg ggcatgctgc cgctcggcgc
    15421 gctggggcgg cggctcccgc tggtcgccct ggccgccctc ccgctggccg tgctcttcgt
    15481 ctggctgggc tgggtgccgg cggcgaacgt cgccaaggtc gtcttcgccg ccgctctcgg
    15541 catctggatc gccggcgagc tcgagcgcgt cagctggatc gtggtcgtgg ccgcggtgtc
    15601 cgccatcgtg gacgtcgtca gcgtcgcggc gggacccacc cgggcgatcc tcgacaaggg
    15661 gccggtcgtc gtcgggtact tcatcgtcgc ggccacgtgg tccgggtact cgtacgacga
    15721 ggccttcacc ggcctcggga cgagcgacgt gctcttcttc gcgctctacc tcggcgcggc
    15781 gcgacgcttc aggctgcgca ccggctggag cgccgtggcg atggtcgcgt cgttcctctg
    15841 caccatcgcc ctcgccatgt ggtggacggc gcttccggcg ctgccgctgc tgtcggtcgc
    15901 cttcctcgcc gtgaacgcgg acctcatcgt gcgggggatg cggcgcgggg gagaggcgcc
    15961 gccggcctga ctgcgtcgcc gccgggtcac cgcccggagc cggacggcgc cgggcgcgct
    16021 caggcgatgc gctcgaacag ggcgacggtc tcgatgtggt gcgtgtgcgg gaacatgtcg
    16081 accggcgcca cgccggtgag ccggtagccg agctcgccga gctcggcggc gttgccggcg
    16141 agcgtggtgg ggttgcacga gacgtaggcg aagcggtcgg cgccgagggc cgcggcgcgc
    16201 tgcagcgcct tgcgcgccag cccggcgcgc ggcgggtcgg cgatcaccac ggccggcctg
    16261 tcggcttcgt ccgcacggcc ggcgtcgagc accgggtgcg gcgggaagcg cagcagcggc
    16321 cgcacgtcgg cggcgtagaa gaccgcgttg tcgacgcggt tgagctccgc gttgacgcgc
    16381 gcggcgtcga tcgcctcccc ctggatctcg atggcgtgca cttcctttgc ggcgcgcgcc
    16441 agcgggaggc tgagcgagcc gatgccgcag tagaggtcca cggcggcgcg gcccggctcc
    16501 ggcgcggcga agcgcagggc gatcgcgtag agcgtctcgc acatggtgct gttggtctgc
    16561 aggaaggcgg cggccggcac gcgcaggtcc acgccggcga ggcgctcccg cagggaggga
    16621 gggccggaca gcatgtgcgg gccgtcgccc acggcggcgt ccgcgcgcga cgcgttcacc
    16681 gtgagcgcga acgaggtgca gccgctcgcc gcggtgacgc gggcggcgag ctcggcctcc
    16741 tccgggaagc gggcggcgac gaagaggttg aggagcaggt cgccgctcgc cagtccctcg
    16801 cggacgacga ggtgccgcag cagcccgccg tcgccgtctc gcgagtaggg ccggaggccg
    16861 aggtggcggc atgcggccgc gaccgccagc ctcgcccggt tcatgcgctg cgaggcgagc
    16921 tcgcagtcga cgatgtcgac gatctcccgc cacgaaccgc gcttgtgcag cccgagcacg
    16981 aggccgccgt gctcgtcctc gccgaacgag aactccatct tgttgcggta ccgccagggg
    17041 tcgtccatcc cgcggatggg ctcgagcctg tactcggtga ggtgggcaag gcgctgcagc
    17101 gagtcgacga cctgctgctg cttgaactcg agctgggcgt cgtaggagag cgtctgccac
    17161 tcgcagccgc cgcactcctg ggcatggcgg cagcgcgccg ccacgcggcg cggcgacggc
    17221 gacaggacct ccagcgtccg cgcctcggcg tgcgactgct tgcgcctggt cacgcgggca
    17281 cgcacgcggt cgcccggcac ggcgccgcgc acgaagacga cgaactcgtc gaggcgcgcg
    17341 acgccctgcc cgccgtaggc gagcgtggcg acgtcgagct cgacgatgtc tccggggcgt
    17401 ggtggcacgg tcagccagtg taccctctcc gcccgggcgc cgcgagcctg tccgggcgtg
    17461 ccccgcggcc gtccgcctcg cacccgatcg tccaccccat gcgccgcctg aggggatgtc
    17521 cgtcgcggcg gccgctggta gagtcgggcc cggaccttcc ggtccgtcgc cggcccgcga
    17581 aaggagtctc cgttgagcga cctcgacctg gagcagtacc gcaggcgcgc cgaggagttc
    17641 gtcggcgctc tcgatctgga gtactacgag cacttctccg gccgcaagcc gcggtgcgac
    17701 acggcggcgg tctacgaccg ctatcccgag ctcttcacgc gcgaggcgat cgacgggctc
    17761 gaggagctct acgccgtcgc cgtcgacgac gagaagctgc gcctcgcgta cctgctcgcc
    17821 ttcaccgtcg acggcttcat gggcgagcag acaaagcacc tcggcgacga gatcgccaat
    17881 acggagggcg aggccaccat caccgtggac ggcgaggtca tcggcctgcg ccaggccagc
    17941 gtcgcgcagg gcaacgagcc ggacagggcg cgccgggccc gcatccagga ggcgcgtctc
    18001 gccgccaccg ccgaacggct caacccgctg cacgacacgc tgtggcggcg ctgccacgac
    18061 ctcgcgctgg agctcgggta tccggactac aaggagctct acggcacggt gcgcgggctc
    18121 gactacgacc acctccgcgc cgagctcgag ggctttctcg ccgacaccga aggcctgtac
    18181 gagcgcgtca tggacaggct ggtccgcgag cgcctcggcg tcaccctctc ggagctcgcc
    18241 ttcgccgacc tgccgttcct gtggcgcgcc gagggcttcg acccggtctt cagggccgac
    18301 gccctcgtgc cctcgctgcg gcgcacgctc gccgggctgg gcatcgacct cgacgcccag
    18361 cccaacgtcc acctcgacac cgaggtccgc gagctcaagt cgccgcgcgc tttctgcgcg
    18421 cccgtgcgcg taccggacga gatctacctg gtggtgctgc cgcacggggg tcaggacgac
    18481 tacggcatgc tgctgcacga ggccgggcac accgagcact tcgcgcacgt gcgccccgac
    18541 ctcgccttcg agtaccggca cctcggcgac aacgccgtga ccgagggatt cgcattcacg
    18601 ttcgaccacc tgatcctcaa ccgcagctgg ctcgcggaca tcctcgactt cgaggccagc
    18661 gacgactacg tgcgcttcgc cgccatcaac gacctgtact tcatgcgccg gtacgccgcc
    18721 aagctggcct acgagaccac gctgcacgcg cagaccggct ccctggaggc catgcccgcg
    18781 gagtacagcc ggcgcctcac ctcggcgacc aaggtcaagg tgccggagga gaactacctc
    18841 gccgacgtcg acgacggctt ctattgcgcc agctacctgc gggcttggat gctcgagggc
    18901 gcgctgcgcc tcatgctgca ggagaacttc ggcatggact ggtacgcatc ccgggacgcc
    18961 ggcgactggc tcaaggcgct gtgggccgag ggccagcacc tcaccgccga gcgcctgctg
    19021 ctgcggcacg ggggcgggcg gctcaacacc gacccgctgc gccacctgtt cgagcaggtg
    19081 ctggggcgct agcagctagg cccgcaggaa cgtcaccagt acgcggcgcc ggcgcggtcc
    19141 gtcgaactcc gcgaagtaga tgccctgcca ggtgcccagc tgcggggtcc ccgccacgac
    19201 ggggatcgtg acgctgctgc ccgtcagggt cgccttggtg tgcgcgtcgg agttgccctc
    19261 cgcgtgcgtg aagggcaggt cgcgcggcac gagccgttcg agcgccatct cgaggtcggc
    19321 gcgcaccgac gggtcggcgt cctcgttgat cgtgaccccg gcggtggtgt gtggcacgaa
    19381 gacgacgacc gtgccgtcac cggcgtctgc gacgagccgg gcgacgtccc tggtgatgtc
    19441 gatgaagctc acgcgctgcc gggtctgcac ctcgagcgtc cacatggttc ctcctagaag
    19501 agtcggaagc gcttgcgcgc ggcggaggcc cggcggcgct gggggaccgt ggcgccctgc
    19561 cgcaacgccc ggacgacgtc cgcggtcgag gtgaccgggt ccgggatctc ggtggagcag
    19621 accatgagct gcgcggggtc gtgagcgtcg ctgccgccgg tgcgggcgac gccgagggcg
    19681 tcggcgatct gctcggcgat gcccacgtag cgcgcgtcga gccacgagcc gttgtacacc
    19741 tcgaccgcgt cgatgacgcc ggcgaccatg ttctcgatgc ccgggtagta ggcggacccg
    19801 ctgcgcccgc cgcccgcccc cgggtgcacc cagacgacgg cggcatggtc ggggacgtcg
    19861 ccggggcgcg gcgcctcctg cacgctgcgc agggtctcca ggtagtcgag gtccggcgag
    19921 aagacgacga aatccccgaa gagggtgctg atctccacgc cgtcgatgag cagcacgccc
    19981 tccgccgcga gcgcagggcc cagccggcgg ttgtcggaga ggtcgccgtg gttggtgagg
    20041 gcgatggcct cgacgtggtt gtcgacgcac caggcgatgt agtcgcggta ggagatgcgg
    20101 gagcagtcgg aggccgggta ggtgtgcgtg tgcaggtcga cgttcatcgg ccccgattat
    20161 agacttcgtg gcaggatgaa agggacactg gacacgttcg gcgtcgtcga gatcctgcag
    20221 atcctgggca ggatgcgccg ctcgggcacc ctccacatcg agtgcccgct gcggctcgtc
    20281 gacgtccact tcacccaggg gcgcatcgcc gagacgcgcg actccacccg cgtcgccgcc
    20341 gacacggtca tcggcagcca gctcctcaag cggtcgctgg tcaacgacca gcagctggcg
    20401 gccgcgctgg ccgagcagga ggtgcggccg cgtcccatcg gcaccatcct cgtcgagcac
    20461 ggcgcggtcg ccgaggacgc gctgcgcgag gtgctctccc gccagatcgc caacaccctc
    20521 gtcgccgcca agctggagga gtccgggtcg ttcgtcttcg tcgtcgaccc cgagccgcag
    20581 ccggtcgcgt acatcacggt cgacacgcac tcggtgctgc tcgacatctc ggcgctggga
    20641 ggggagtact gtctggcggt cgagatgctc ggccagccgt ccacggtgct cgtccgcaac
    20701 ggcgactaca acacgctgcc gaggaacccg ctgctcatgg gccgggacga gttcgccgtg
    20761 ctgctgcagg tggacggcac gcgcacggtc aaggagatca cgcaggccag ccgtctcgag
    20821 gagatcaccg tggtgagcat cctcggcaag ctggccgacg cgggcgtgct gctggtcaag
    20881 gcggagcgcc actcgcgcgc ggaggacgcc gcggagcttc aggcgcaccg cgacagcgtg
    20941 tgggcggagg tcagccacct tctcgacgac atggtcgagg agccggatgc cggagcgccc
    21001 g
//
LOCUS       DASUMX010000096         6412 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1463020, whole genome shotgun sequence.
ACCESSION   DASUMX010000096 DASUMX010000000
VERSION     DASUMX010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 6412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6412
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1463020"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1906
                     /locus_tag="VFZ86_04735"
     CDS             <1..1906
                     /locus_tag="VFZ86_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEX5641630.1"
                     /translation="WFTFLPAWMHNGYFLWALASVVQFWVGWQFDTTAGAALKHGTTT
                     MNTLVALGSSAAYLYSVLGVLFPAFFEHQGLGEPMYFDSSAFIITLILLGRLLEARAK
                     GQTGAAIKALIGLQPKTARVIRGGAETDVPIAQVLVGDLIVVRPGEKVPVDGLVEEGS
                     SAVDESMLTGEPIPVGKHPGDEVIGATMNTTGSFTFRATKVGSETALAQIVRLVEQAQ
                     GSKPPIARLADVIAAYFVPVVIVIATLTLVVWLVFGPSPALNYALLNFVAVLVIACPC
                     ALGLATPTAIMVGTGKGAENGVLIRDGAALETAHKLDVVVLDKTGTITEGKPRVTDVV
                     LFGSGHGTVFATGDRLDNTELLRLVAGAERGSEHPLAVAILAAAAERGIEAPAATGFE
                     AIAGHGIRAQVDGHTVVAGNERLAGHAGERAAQLAGQGKTVVTVMVDDEPVGAIAIAD
                     TVKPSSAAAVGRLRQLGLDVVMLTGDHEATARAIAAQVGIDKVVAEVLPEQKAAKVAE
                     LQQDGSRVAMVGDGINDAPALAQADVGIAIGTGTDVAMEASDVTLMSGDVGGVATAVD
                     LSRATIRTVKQNLFWAFAYNVALIPLAAGVFYPAFGILLSPIWAAAAMGLSSVTVVSN
                     SLRLRRFRPG"
     gene            2335..3003
                     /locus_tag="VFZ86_04740"
     CDS             2335..3003
                     /locus_tag="VFZ86_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HEX5641631.1"
                     /translation="MLQVLDRHAIGHILRTKEVDAWGVAANDPRLPLAPGLPRAIAIL
                     MRLDPLVVRGLRHGPTQDYEQEYLRLNGALDDATGTLVDVLHVHGHAAERVAATTDDG
                     NGGKPFPHKTAATSAGLGWIGKTALFVSPEFGPAVRLATVFTDLELPAGDPVRESSCG
                     ECRACVDACPAGCGRDVLWQPGMARELLFDAAACRHHMASFTSLESEICGICIAACPY
                     ALRH"
     gene            3194..3628
                     /locus_tag="VFZ86_04745"
     CDS             3194..3628
                     /locus_tag="VFZ86_04745"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEX5641632.1"
                     /translation="MSFDTSQVTADLAARGLRLTRQRRAVLDAIAAAPSSLSALQVYD
                     AARSRCPELGLTTVYRTLEVLTELGALRRVHGSDHCESFVPASAAHGHTVVCSACGRV
                     TEFTACDMRGIADAAAQETGYCITDHFLQLGGLCAACRKAAS"
     gene            3625..4557
                     /locus_tag="VFZ86_04750"
     CDS             3625..4557
                     /locus_tag="VFZ86_04750"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEX5641633.1"
                     /translation="MKRGPLVLLAAALLVVPAGLLGGCSAQDDAADTGALDVLASASF
                     LADIAQNVAGDRFEVRSLVPLETDPHSFEPTPSDLRDVAAADLLIVNGGGLEGSLLDT
                     LESAGGDATLVDASAGLAGRTPQPGEPALEDGDADPHFWLDPVLVKTYVATIRDAFAA
                     ADPAGAAEYEANAAAYQRQLDELDAWIAAQIAEIPETQRKLVMNHTSHGYFADRYGLE
                     IVGTVLPGASTGETPTARGLSDLVRAIESSGARAIFVEIDESPDLARQIAAETGVAVV
                     ADLLDHSLTEPDGPAPTYIDMMKYDTRRIVEGLR"
     gene            4554..5384
                     /gene="aztA"
                     /locus_tag="VFZ86_04755"
     CDS             4554..5384
                     /gene="aztA"
                     /locus_tag="VFZ86_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein AztA"
                     /protein_id="HEX5641634.1"
                     /translation="MSAATAAARLTLDGVTVGYDGRPALEDVTMSVPHGAQVAIVGPN
                     GAGKSTLFKALVGLLPVRSGRVLLHDREPGGQADPVAYVPQREEIDWGFPVTVHDVVM
                     MGRYGRLGWFRRPQAADREVVARCLDELGIAELEKRAIGELSGGQQQRVFLARALAQE
                     PHVLLLDEPFTGVDVSARETLLTLLARLRAQGITVLVSTHDMQTAADRFELVALLNRR
                     LIAYGAPSDVFTPAHLSEAFGGQALFLNGMVVIDQCCPGHVGDGEAHRHSHEPHAGPR
                     "
     gene            5381..6229
                     /locus_tag="VFZ86_04760"
     CDS             5381..6229
                     /locus_tag="VFZ86_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEX5641635.1"
                     /translation="MIAWFTEPFTLEFMQRALVASLIVGVLCSVIGTFVVLRAMAFLG
                     DALAHAILPGVAVAYLLGANLLLGALVAAVLVAVGIGVFSRRGGVKEDTAIGILFAAA
                     LALGVLLMSTVRSYSADLTHILFGNVLGVSAGDLWLTGVLAVIVLTVLIVFFKEFQLT
                     SFDPVLAHMLGKRPETLRFAMLILLALTIVVGLQTVGVGLVAAMLVTPAATAYLLTRR
                     LAAMMAVSAVVGAASSVAGLYASYYLDVASGAAVVLIATAAFLLAYVFAPRRGLVARH
                     VARRAA"
BASE COUNT          850 a         2417 c         2187 g          958 t
ORIGIN      
        1 gtggttcacg ttcctgccgg cgtggatgca caacggctac ttcctctggg cgctcgccag
       61 cgtcgtgcag ttctgggtgg gctggcagtt cgacaccacc gccggggcgg cgctgaagca
      121 cggcaccacg accatgaaca cgctcgtcgc cctgggctcc tcggcagcgt acctctacag
      181 cgtcctcggc gtgctcttcc ccgcgttctt cgagcaccag ggcctcggcg agcccatgta
      241 cttcgactcc tcggccttca tcatcacgct catcctcctc gggcggctgc tcgaggcgcg
      301 cgccaagggg cagaccggag ccgcgatcaa ggcgctgatc gggctgcagc ccaagacggc
      361 gcgcgtgatc cgcggcggcg cggagaccga cgtgcccatc gcccaggtcc tggtcggcga
      421 cctcatcgtc gtgcggcccg gcgagaaggt gccggtggac ggcctcgtcg aggagggctc
      481 ctcggcggtc gacgagtcca tgctcaccgg cgagccgatc cccgtgggca agcacccggg
      541 cgacgaggtg atcggcgcca ccatgaacac caccggctcg ttcaccttcc gcgcgaccaa
      601 ggtgggttcc gagaccgccc tggcgcagat cgtgcgtctc gtggagcagg cgcaggggtc
      661 caagccgccg atcgcgcggc tggcggacgt gatcgccgcg tatttcgtcc ccgtcgtgat
      721 cgtcatcgcg acgctcacgc tggtcgtgtg gctcgtgttc ggcccgtcgc cggcgctgaa
      781 ctacgcgctg ctcaacttcg tcgcggtgct cgtcatcgcg tgcccgtgcg ccctcgggct
      841 ggccacaccg acggcgatca tggtcggcac cggcaagggc gccgagaacg gcgtgctcat
      901 acgcgacggc gccgcgctcg agacggcgca caagctggac gtcgtcgtgc tcgacaagac
      961 gggcacgatc accgagggca agccgcgggt gaccgacgtg gtgctcttcg gctccggcca
     1021 cgggaccgtc ttcgcgaccg gcgaccgcct cgacaacacc gagctcctgc ggctggtggc
     1081 cggcgcggag cgcggcagcg agcacccgct ggccgtcgcg atcctcgccg ccgccgcgga
     1141 acgcggcatc gaggcgccgg cggcgaccgg gttcgaggcg atcgccggac acggcatccg
     1201 cgcgcaggtc gacggccaca cggtggtcgc cggcaacgag cggctcgccg gccacgccgg
     1261 ggagcgagcc gcccagctgg ccggccaggg caagacggtc gtcaccgtca tggtggacga
     1321 cgagccggtc ggcgccatcg cgatcgccga cacggtgaag ccgagctccg cggcggccgt
     1381 cggccgcctc agacagctcg gcctcgacgt cgtcatgctc acgggcgacc acgaggcgac
     1441 ggcgcgcgcc atcgccgccc aggtcggcat cgacaaggtg gtcgccgagg tactgcccga
     1501 gcagaaagcc gccaaggtgg ccgagttgca gcaggacggc agccgggtcg ccatggtcgg
     1561 cgacggcatc aacgacgcgc cggcgctcgc ccaggcggac gtcggtatcg ccatcggcac
     1621 ggggacggac gtcgccatgg aggcgagcga cgtgacgctc atgtcgggcg acgtcggcgg
     1681 cgtggccacg gccgtggacc tctcgcgcgc cacgatccgc acggtcaagc agaacctctt
     1741 ctgggcgttc gcctacaacg tcgccctgat cccgctggcg gcgggcgtgt tctatccggc
     1801 gttcggcatc ctgctgagcc cgatctgggc cgcggcggcg atggggctga gcagcgtcac
     1861 ggtggtgtcg aacagcctgc ggctgcggcg cttcaggccg ggctgacggg tcgggcccgt
     1921 acgcgacgag aggggcggta cccgatcatg ggaccgcccc tctccggtgg cgatgctacg
     1981 ttgtcggtcg atccgttcat catccatcgc gatcacctcc ggcgcttgcc gtagcgatcc
     2041 tcctgcttca ccttcgtcct gctgaagtgc acaggtccca tgatctcacc tcctccgcgt
     2101 ctcggtctgc acctctcaag ctgccctgct cctatgaaaa taaacctcgt atgtgaaagt
     2161 ctgcacgttt cgttgccgac gcggcctttc gcctccgcgt gccaatacgg acaaccccgg
     2221 cgacaaaaac cgttcccccg tccattgtcg ccgccgagcc gtgggtgcga ggatgaacgg
     2281 ggtcgggggg ccgtccgccc gtccgggcac cccagtccac cacgaagagg tcacatgctc
     2341 caggttctcg accggcacgc gatcgggcac atcctccgca ccaaggaggt cgacgcctgg
     2401 ggcgtggccg ccaacgatcc gcgactgccg ctggccccgg gcctgcccag agccatcgcc
     2461 atcctcatgc ggcttgaccc gctggtcgtg cgtggcctgc gccacggccc cacgcaggat
     2521 tacgagcagg agtacctgag gctgaacggc gcgctcgacg acgccaccgg gacgctcgtc
     2581 gacgtgctgc acgtgcacgg acacgccgcc gagcgcgtag ccgccacgac cgacgacggc
     2641 aacggcggca agccgttccc gcacaagacg gccgccacct cagcgggact cggctggatc
     2701 ggcaagacgg ccctgttcgt ctcgccggag ttcggccccg ccgtccgcct ggccaccgtg
     2761 ttcaccgacc tcgagctgcc cgccggcgac cccgtgcgcg agagcagctg cggggagtgc
     2821 cgcgcctgcg tcgacgcctg ccccgccggg tgcggccggg acgtgctctg gcagccgggc
     2881 atggcccgcg agctgctctt cgacgccgcc gcctgccgcc accacatggc gagcttcacg
     2941 agcctcgagt cggagatctg cggcatctgc atcgcggcct gcccctacgc cctgcggcac
     3001 tgacgccgcc ggccggccgc cgccggccgg cccctcgaac ctgatccgcc gcccccgccc
     3061 ggtcccaacg cccttccacc ctgacgtcgt ccttcgcccg gtccgtcacg ccccgccttg
     3121 aagcgcctcc ttccccgtgc taggctcgcc gctctttagt gaaagtggtt ttcagtttca
     3181 gaaaggacgt gcggtgagct tcgacacctc ccaggtcacg gccgacctcg ccgctcgcgg
     3241 cctgcgcctt acccgccagc gccgggccgt gctcgacgcc atcgctgcgg ccccctccag
     3301 cctgagcgcg ctgcaggtgt acgacgccgc gcgctcccgg tgccccgagc tcgggctcac
     3361 caccgtgtac cggacgctcg aggtgctgac cgagctgggc gcgctgcgcc gggtgcacgg
     3421 gtcggaccac tgcgagagct tcgtgcccgc cagcgccgcg cacggccaca ccgtggtctg
     3481 cagcgcctgt ggccgggtca ccgagttcac cgcctgcgac atgcgcggca tcgccgacgc
     3541 cgcagcgcag gagaccggct actgcatcac cgaccacttc ctgcagctcg gcgggctgtg
     3601 cgccgcgtgc agaaaggccg cctcgtgaag cgcggcccgc tggtccttct cgcggccgcg
     3661 ctgctcgtcg ttccggccgg actgctcggc ggctgctcgg cgcaggacga cgccgcggac
     3721 acgggcgcgc tcgacgtcct ggcctcggcg agcttcctcg ccgacatcgc gcagaacgtc
     3781 gccggcgacc gcttcgaggt gcgctcgctc gtgcccctgg agacggaccc gcactccttc
     3841 gagcccacac cgtcggacct ccgcgacgtc gccgcggcgg acctcctcat cgtcaacggc
     3901 ggcggtctcg agggctcgct gctcgacacg ctcgagagcg ccggcggcga cgcgacgctg
     3961 gtcgacgcct ccgccggcct cgccggccgc acgccgcagc ccggcgagcc cgctctcgag
     4021 gacggcgacg ccgacccgca cttctggctg gacccggtgc tggtcaagac gtacgtcgcc
     4081 acgatccggg acgccttcgc cgccgcggac cccgccgggg cggcggagta cgaggccaac
     4141 gccgccgcct atcagcggca actcgacgag ctcgacgcct ggatcgctgc gcagatcgcg
     4201 gagatccccg agacccagcg caagctggtc atgaaccaca cgagccacgg ctacttcgcg
     4261 gacaggtacg gcctggagat cgtcggcacc gtcctgccgg gcgcgagcac gggcgagacc
     4321 ccgacggcgc gcgggctctc cgacctcgtc agggcgatcg agtccagcgg ggcacgcgcc
     4381 atcttcgtgg agatcgacga gagtcccgac ctcgcccgcc agatcgcggc ggagaccggg
     4441 gtcgcggtgg tcgccgacct gctcgaccac tcgctcaccg agccggacgg cccggcgccg
     4501 acgtacatcg acatgatgaa gtacgacacg cggcggatcg tggagggcct gagatgagcg
     4561 ccgcgaccgc cgcggccagg ctcacgctcg acggcgtgac cgtcggctac gacgggcggc
     4621 cggcgctcga ggacgtcacg atgagcgttc cgcacggcgc ccaggtggcg atcgtcggac
     4681 cgaacggcgc cggcaagtcg acgctcttca aggcgctcgt gggcctcctc cccgtgcgct
     4741 ccgggcgcgt gctcctgcac gatcgcgagc cgggcggcca ggctgacccg gtcgcctacg
     4801 tgccgcagcg agaggagatc gactggggct tcccggtcac ggtgcacgac gtcgtcatga
     4861 tgggccggta cgggcgcctc ggctggttcc gtcgtccgca ggccgccgac cgcgaggtgg
     4921 tggcgcgctg cctggacgag ctcggcatcg ccgagctcga gaagcgcgcc atcggcgagc
     4981 tctccggcgg ccagcagcag cgcgtgttcc tcgcccgcgc cctcgcccag gagccgcacg
     5041 tcctgctcct ggacgagccc ttcaccggcg tcgacgtgag cgcgcgcgag accctgctca
     5101 cgctgctcgc gcgcctgcgc gctcaaggga tcacggtgct ggtcagcacg cacgacatgc
     5161 agacggcggc ggaccggttc gagctcgtcg ccctcctgaa cagacggctg atcgcctacg
     5221 gcgcgccgtc cgacgtgttc actccggcgc acctcagcga ggcgttcggc ggccaggcgc
     5281 tcttcctcaa cggcatggtc gtgatcgacc agtgctgccc cggtcacgtc ggggacggcg
     5341 aggcccaccg gcacagccac gagccacacg cggggccgcg atgatcgcct ggttcaccga
     5401 gccgttcacg ctggagttca tgcagcgcgc gctcgtcgcc tcgctgatcg tcggcgtgct
     5461 ctgctcggtg atcgggacgt tcgtcgtgct gcgcgcgatg gcgttcctcg gcgacgcgct
     5521 ggcccacgcc atccttcccg gcgtcgccgt cgcctacctg ctcggcgcga acctcctgct
     5581 cggcgcgctc gtcgcggccg tgctcgtcgc cgtgggcatc ggcgtgttct cgcgccgcgg
     5641 cggcgtcaag gaggacaccg ccatcggcat cctcttcgcc gcggccctcg ccctcggcgt
     5701 gctgctcatg agcacggtcc ggtcgtactc cgccgacctc acgcacatcc tcttcggcaa
     5761 cgtcctcggg gtgagtgcgg gagacctctg gctgaccggg gtgctcgcgg tgatcgtgct
     5821 gacggtgctc atcgtcttct tcaaggagtt ccagctgacg tcgttcgacc ccgtgctcgc
     5881 gcacatgctg ggcaagcggc cggagacgct gcgcttcgcc atgctcatcc tgctggccct
     5941 gaccatcgtg gtgggactgc agaccgtggg cgtggggctg gtcgccgcca tgctggtcac
     6001 cccggcggcg accgcgtacc tgctcacgcg gcggctggcg gcgatgatgg ccgtctccgc
     6061 cgtggtcggc gccgcctcga gcgtcgccgg cctgtacgcg agctactacc tcgacgtggc
     6121 gtcgggggcg gcggtcgtgc tcatcgccac cgccgccttc ctgctcgcgt acgtcttcgc
     6181 gccgcgccgc gggctggtcg cccgccacgt cgcgcggcgc gccgcctgac tcgccgcacg
     6241 gcctgacccg ccgcgcgctt ccccgccccg ccgcgtgctg ccggtcaggg tcacacaggc
     6301 tccacgcggg atcccttcgc gtccgggcgt cctatggtgc tccacggact gtcacgcccg
     6361 aaggtacgcg caggtgtgca tgtgtactcc tgcgcgtact tccgggcgtg cg
//
LOCUS       DASUMX010000097        12420 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1175839, whole genome shotgun sequence.
ACCESSION   DASUMX010000097 DASUMX010000000
VERSION     DASUMX010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 12420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12420
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1175839"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..120
                     /locus_tag="VFZ86_04765"
     CDS             <1..120
                     /locus_tag="VFZ86_04765"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEX5641636.1"
                     /translation="ITGRRAPEELIAAADLATEMRPLKHYFDAGVQARAGIEY"
     gene            208..1512
                     /gene="lysA"
                     /locus_tag="VFZ86_04770"
     CDS             208..1512
                     /gene="lysA"
                     /locus_tag="VFZ86_04770"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011231782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEX5641637.1"
                     /translation="MYPHTSAHNAAGHLEIGGCDVVDLAREHGTPLFIYDEQTLRDQC
                     RAYHTAFGARTGLYEIVYASKAFSCRAMAELVAQEDLSLDVATGGELAAALAGGFPAG
                     RIYFHGNNKSPAEIDAGLDAGIGHFVVDSFEEIERLEAAAAARGRSQDVLVRVTPGVR
                     PDTHDFIQTGQQDSKFGFGLADGLAHEAVRRLKDAAHLELVGVHAHIGSQIFELDSYR
                     REVEVLFVAIDDWRRDFGFVCRIFNIGGGLGIRYTEADQPSSIAEFAGIAVDAVRAGA
                     EKHGMPMPRLFVEPGRSIAGKAAVTAYTVGTVKVIPGVRTYVAVDGGMSDNLRPMLYD
                     SRYEAMLANKAEAEATDVVTVAGKHCESGDVLVRDVYVAPPEPGDILVTPGTGAYGYA
                     MANNYNAQPRPAVVLVGGGAARVIIARETWDDVLRLQRPLAP"
     gene            1541..2824
                     /locus_tag="VFZ86_04775"
     CDS             1541..2824
                     /locus_tag="VFZ86_04775"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEX5641638.1"
                     /translation="MDKVGIGLLGYGTVGSGVGKLLARNAGDITLATGKEVALRRVLE
                     KDPGFSHPDLDPALLTTRFEDILDDPEISVVVELIGGTGAARDFQLRALKAGKHVVTA
                     NKQLLAQHGAELFAAAEESGAHLRFEASAVGAVPVIKVLRESLVAAEVKTVFGIVNGT
                     TNYMLSAMAETGAEYDDVLEEAQRLGYAEADPTEDVGGKDAAAKMAILSSIAFHSRTG
                     LDDVPYEGITKVSATDIAHGGRLGLVAKLLGVAKLIDGRMNVRVYPAFIPRTHPLAGV
                     TGAYNAVFLTSDAFDKIMLFGPGAGSVPTASAVIGDVISVVNTVKGSFVQNCLCYKEL
                     PFFPDAEMVSKFYLRLRVNDQPGVLAKVSALFGEHGVSIHSMVQEGGGDEAELVLLLH
                     PVREAQFFAALEQIEALPDVHGEPAVIRVEGEETA"
     gene            2821..4014
                     /locus_tag="VFZ86_04780"
     CDS             2821..4014
                     /locus_tag="VFZ86_04780"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HEX5641639.1"
                     /translation="MTEKKGRIGILTGGGDIPGLNQAIRGVTLRALSEGFEVVGIRRG
                     WAGLVELKRDTAADNSEWVLPITRELVERYAYSGGTFLHSSRTRPSTVPAKAVPEHLK
                     GTYSAEKNDLTDEVIANLEFLGIDYLVPAGGDDTLSYGVELGRRGFPVVAIPKTMDND
                     VPGTDYCMGFGTCVNRTIEMARQVTSSAASHERIVVLEVFGRYAGFTALLPTFAGAAD
                     RCVIPEAEFDMERLTELLVADRNGRPEGYSVCIVSEGAMPAGGEMVFMGGEKDMFGHA
                     KLGGIGQNVSNKIKELAPAYNGGKKIDMISMNLSYLVRSGAPDAIDCIVPTAYGNLAV
                     DLILKGQTGRMVALQGGRYGSVPIDEVVSTKKVVDVGRYYDTERYRPKYDALELSPMM
                     VLGGS"
     gene            4014..5192
                     /locus_tag="VFZ86_04785"
     CDS             4014..5192
                     /locus_tag="VFZ86_04785"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HEX5641640.1"
                     /translation="MRKRVLLVPDGMADEPQAALGGRTPLEAASTPAMDCLARGGICG
                     LAHTVPDGMSPGSDVANLAVLGYDPAAVFSGRSPLEAASLGVELGADDVSYRLNLVTV
                     GGGLMKDNTAGHIGSDDARRCIEALQAELGSDAYEFHLGVSYRHLFVWRGGALVPCTP
                     PHDILDRPVAGHLPGEAAGAAGGGSPAAELAALQARASEVLERVRPGTSAWFWGEGTA
                     PRISPFRDHFGLSAAVVGAVDLVRGIGRLAGLDVIDVPGATGGLDTDYGAKARAAIAA
                     LADHDLVWVHVESPDEAGHMGDLREKVRAIERVDAEILAPILASAGRPAVLVVPDHLT
                     PLRTRTHAAGPVPFAIGDSLPGAGGGGAMAYGESQAAMTGLVVEDGAALMRLFLEATG
                     "
     gene            5264..7474
                     /locus_tag="VFZ86_04790"
     CDS             5264..7474
                     /locus_tag="VFZ86_04790"
                     /inference="COORDINATES: protein motif:HMM:NF026092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching N-terminal
                     domain-containing protein"
                     /protein_id="HEX5641641.1"
                     /translation="MSSSHEQPERQPARERPADEPVVTTTSAERYVMSGGRTSRLAVK
                     EDDLFLYTNELGQVPGTENSVLGLYYRDTRYLSRYVLSIAGRQPVLLSASGERGYAAT
                     IELTNLEARTADGRVLPQASVHVRRTRFVSDRLYELLRVRNYHQREVDLVLDLDFDAD
                     FADLFEVRGSRRRRRGSRLAPRVSDGVLTLSYLGLDDVTRQTIVRFHDRPESLKQGRA
                     RFRLRLPPGERAVIRYDVQVVGPEAPGEDGGEFNARIGALRHEHERWASGATDIFTDN
                     DQLNRVLRRGQDDLRLLSTVIDGERVPLSGLPWFGAPFGREMTFVGLETLLLDLRWAR
                     SAVSFLARRQGVQDSAFREEQPGKIMHELRRGELAAIRAVPHTPYYGSVDSTALWLLL
                     VAELAMWTGDLDEFELRAAAVDAALGWIDGPGDPDGDGFVEYERRSRVGLRNQGWRDS
                     FDAVLHEDGTPATGPIALAETQGYVYYAKRRLAAVFGQLGDVERAERLSQDAARLKRA
                     FNERFWVEDQGFYAMALDGQKRQVRSASSTIGHALWSRIVAEEHVPAVVRRLMAPDMF
                     TGWGIRTLSKEARYYNPVSFYNGSVWPFDTALIANGLKKHGYVQEANRLAWGLVEAAS
                     AHEYARLPEMFCGFTRQTVDRPVSFPMACSPDANSAAALYLVLQSMLGIYAQAEENIV
                     YVHNPVLPRWLGEVTLSNLRVGRTTMRLRFRREGGQTSFSVLDKQGPGRIVVVE"
     gene            7479..8255
                     /locus_tag="VFZ86_04795"
     CDS             7479..8255
                     /locus_tag="VFZ86_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013457456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX5641642.1"
                     /translation="MLTIAHISDIHAGSPYFVPSLMDRVVVELNELEPDVVLCTGDLT
                     EMGFRQDYVTAKEYMDRIQAPLVVIPGNHDARNVGYVHFEELIGPRWQTLSLGGVKIV
                     ALDSSEPDLDYGQIGRARYDFLREEFGRPAGFRIFMLHHHVLPIPGTGRERSMALDAG
                     DLLEVLQECGVNLVLSGHKHVPYAWRLEHLFVVNAGTCSSLRLRGKTKACYNLVRIDD
                     DRVQVSRKYPFHGEETLIDFVPSTAAFDKYICPAPREESR"
     gene            8309..9688
                     /locus_tag="VFZ86_04800"
     CDS             8309..9688
                     /locus_tag="VFZ86_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate synthetase"
                     /protein_id="HEX5641643.1"
                     /translation="MVAAAVARLEDAYAVAGGIFAGGREKLRGGEAGGLAALAAEIGV
                     PRLDAVEPRSASAHEVLEGVRAVLRALRAEALVDLSDEPVVGYRERFLLMSAALAEGA
                     AYVTSDTEVRPQAFARLAATPSLAVIGTGKRVGKTAVSGWLARRLDAARRADGGVVVL
                     AMGRGGPPEPELIQGGGLGPAELLAASRAGRHAASDCYEDAVLAGVTAVGCRRCGGGL
                     AGMPFDDNVREALPLLDGRGAALAVIEGSGAVVPPVAADATLCVAGASQPADYVAGYL
                     GTYRLLLSDALVLTQCETPFASPGEVAAMAAAAGAVRPGLEVVRTVFRPRPAQSVRGR
                     RVAFFTTAPEAAAPRLAASLAEEHGAEVVLVSCDLADRRRLREAVARAAAEADVFLTE
                     IKAAAVDVVAEGAAAAGRELVFCDNEPVALEGDLAATVDRLAAFAAERFAARADASQE
                     RSADGAPGD"
     gene            9691..10635
                     /locus_tag="VFZ86_04805"
     CDS             9691..10635
                     /locus_tag="VFZ86_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641644.1"
                     /translation="MPEPHDIPLIDEGDQAPFSKGLMAQTLMATGLAPERAYNVAAAV
                     ERRLRRRGPAALTLADLQEIARDQLGPEKGDVLIERFRQWQKLRRLETPLIILIGGAT
                     GVGKSTLATQLAHRLGITRLIGTDMVRQTMRAFFAAELMPAIHTSSFDAAAAVRVPVP
                     RETDLSKMGFIEQTKAVSVGIEALVQRGIDEAQRMVVEGVHLVPGFLDRSRWGEAIVL
                     EFMLAISDKERHRGNFTVREWETGGIRPLRRYIEHFAEIRRIQKYMVGRAQALGVPVI
                     DGPSLDDNLSEVLGMILRRVEEEGGEPEPAGEGKAPPA"
     gene            10900..>12420
                     /gene="rho"
                     /locus_tag="VFZ86_04810"
     CDS             10900..>12420
                     /gene="rho"
                     /locus_tag="VFZ86_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014138080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEX5641645.1"
                     /translation="MSTEEQTDQMTVPSEPAVETAVAPPVAEKPVKDKPAEKPARASR
                     AKAAPAAAKAAGAASKAAEALAPVKAAEAATAPAAPAPPAAEAAATAPAAYKPAEAAA
                     PAPAADKPAEATAQAPAADKPAEAPAAETQPAAPAAEATAAGAPAAAPEAPAADRPAG
                     DGERPQQQGGQQQGQQQRQGKGKNRRGRDKRQGGGGGGGDQQRDDRPTIPCTGILDVL
                     PDGFGFLRTNGFTQGDRDVYVSLSQIRRFGLRRGDLVAGQVREPKDNEKYNALLKIDS
                     VNGIDPDTARKRPVFEQLTPLFPEERLRLETDEHDTAARIMDLMCPIGKGQRGLIVSP
                     PKAGKTTILKKLANSIAVNNPDVDLFVLLVDERPEEVTDMQRSVIGTVVASTFDQPSE
                     NHVQVTELVLDRVKRLVEMGHDVVVLLDSITRMARAFNLNTPASGRILSGGVDSTALY
                     PPKKFFGAARNIEEGGSLTILATALVDTGSRMDEVIFEEFKGTGNMEIRLDRKLADK"
BASE COUNT         1858 a         4490 c         4436 g         1636 t
ORIGIN      
        1 atcaccggcc gccgcgcgcc cgaggagctc atcgcggcgg ccgacctggc caccgagatg
       61 cggcccctca agcactactt cgacgccggc gtgcaggcgc gcgccgggat cgagtactaa
      121 tgcacggaag agctattgag cgaccgccgg cccgcagggc cccgaggcgg acggactgag
      181 cgcagccggc ccatgacgca cacgctcatc tacccgcaca ccagcgcgca caacgccgcc
      241 ggccacctcg agatcggcgg ctgtgacgtc gtcgacctgg cgcgcgagca cggcacgccg
      301 ctgttcatct acgacgagca gacgctgcgc gaccagtgcc gcgcctacca caccgccttc
      361 ggcgcgcgca ccggcctcta cgagatcgtg tacgccagca aggcgttcag ctgtcgcgcc
      421 atggcggagc tggtggcgca ggaggacctg tcgctcgacg tcgccaccgg cggcgagctg
      481 gctgcggcgc tggccggcgg attccccgcg gggcgcatct acttccacgg caacaacaag
      541 tcgccggccg agatcgacgc cggcctcgac gccggtatcg gccacttcgt cgtcgactcg
      601 ttcgaggaga tcgagcggct ggaggcggcc gcggccgccc ggggccgctc ccaggacgtg
      661 ctcgtgcgcg tcacgccggg cgtgcgcccc gacacgcacg acttcatcca gaccggccag
      721 caggacagca agttcggctt cgggctggcc gacggcctcg cgcacgaggc ggtgcgccgc
      781 ctcaaggatg cggcgcacct ggagctcgtc ggcgtgcacg cgcacatcgg ctcccagatc
      841 ttcgagctcg actcgtaccg gcgcgaggtc gaggtgctct tcgtcgccat cgacgactgg
      901 cgccgtgact tcggcttcgt ctgccgcatc ttcaacatcg gcggcgggct cggcatccgc
      961 tacaccgagg cggaccagcc cagctccatc gccgagttcg cggggatcgc cgtggatgcc
     1021 gtgcgcgcag gggcggagaa gcacggcatg ccgatgccga ggctcttcgt cgagccggga
     1081 cggagcatcg ccggcaaggc ggcggtcacc gcgtacactg tggggacggt caaggtcatc
     1141 cccggcgtgc gcacctacgt ggcggtggac ggcgggatga gcgacaacct gcggccgatg
     1201 ctgtacgact ccagatacga ggccatgctc gcgaacaagg cggaagcaga ggcgaccgac
     1261 gtggtcaccg tcgccggcaa gcactgcgag tcgggcgacg tgctggtgcg cgacgtgtac
     1321 gtcgcgccgc ccgagccggg agacatcctg gtcacgcccg gcaccggcgc ctacggctac
     1381 gccatggcca acaactacaa cgcgcagccg cgcccggccg tggtgctggt cggcggcggc
     1441 gcggcgcgcg tcatcatcgc gcgagagacc tgggacgacg tgctgcgcct gcagcggccg
     1501 ctcgccccgt gacgacccca cgaccgcgga gggcacgaca gtggacaagg taggcatcgg
     1561 gctcctcggc tacggcacgg tggggtccgg cgtgggcaag ctgctcgcca ggaacgccgg
     1621 cgacatcacc ctggcgaccg gcaaggaggt cgcgctcagg cgcgtgctcg agaaggaccc
     1681 gggcttcagt caccccgacc tcgaccccgc gctgctgacg acgcgcttcg aggacatcct
     1741 cgacgacccg gagatctcgg tggtcgtcga gctcatcggc gggacgggcg cggcgcgcga
     1801 cttccagctc cgcgccctca aggccggcaa gcacgtggtg acggccaaca aacagctcct
     1861 ggcccagcac ggggccgagc tcttcgccgc cgcggaagag agcggcgcgc acctccgctt
     1921 cgaggccagc gccgtcggcg ccgtccccgt catcaaggtg ctgcgcgagt cgctggtcgc
     1981 cgcggaggtc aagacggtgt tcggcatcgt caacggcacg accaactaca tgttgagcgc
     2041 gatggccgag acgggggcag agtacgacga cgtcctcgag gaggcccagc ggctcggtta
     2101 cgccgaggcc gatcccacgg aggacgtcgg gggcaaggac gcggccgcca agatggcgat
     2161 cctcagctcg atcgccttcc acagccgcac cggcctcgac gacgtaccct acgagggcat
     2221 caccaaggtg tcggccaccg acatcgccca cggagggcgg ctcggcctgg tcgcgaagct
     2281 cctcggggtg gccaagctca tcgacgggcg tatgaacgtg cgcgtctatc ccgccttcat
     2341 ccccaggacc cacccgctcg ccggcgtgac cggcgcctac aacgccgtct tcctgaccag
     2401 cgacgcgttc gacaagatta tgctgttcgg ccccggcgca ggcagcgtcc ccacggcgtc
     2461 cgccgtcatc ggggacgtga tctccgtggt caacacggtg aagggaagct tcgtgcagaa
     2521 ctgcctctgc tacaaggaac tgccgttctt ccccgacgcg gagatggtat cgaagttcta
     2581 cctcaggttg agggtgaacg atcagccggg cgtgctcgcc aaggtcagcg cgctgttcgg
     2641 cgagcacggc gtgagcatcc attccatggt ccaggagggc ggcggggacg aggccgagct
     2701 cgtgctgctg ctccacccgg tccgcgaggc ccagttcttc gccgcgctcg agcagatcga
     2761 ggccctgccg gacgtgcacg gcgaaccggc cgtgatacgc gtggaaggag aggagacagc
     2821 atgactgaga agaagggccg catcggcatc ctcaccggcg gcggcgacat ccccggtctc
     2881 aaccaggcga tccgcggcgt caccctgcgc gccctcagcg aggggttcga ggtggtcggc
     2941 atccgccgcg gctgggcggg tctcgtcgag ctcaagaggg acacggccgc cgacaacagc
     3001 gagtgggtgc tcccgatcac cagggaactc gtcgagcgct acgcgtactc gggcggcacg
     3061 ttcctccact cgtcgcgcac ccgccccagc acggtgccgg ccaaggccgt cccggagcac
     3121 ctcaagggca cgtactccgc cgagaagaac gacctcacgg acgaggtgat cgcgaacctc
     3181 gagttcctcg gcatcgacta cctcgtcccc gcgggcgggg acgacacgct cagctacggt
     3241 gtggagctcg gccgccgcgg gttccccgtc gtcgccatcc ccaagaccat ggacaacgac
     3301 gtacccggca ccgactactg catgggcttc ggcacctgcg tcaaccgcac catcgagatg
     3361 gcgcgccagg tgaccagctc tgcggcttcg cacgagcgca tcgtcgtgct cgaggtcttc
     3421 ggccgctacg cgggcttcac ggcgctgctg ccgaccttcg ccggcgccgc cgaccgctgc
     3481 gtgatcccgg aggccgagtt cgacatggag agactcaccg agttgctcgt cgccgaccgc
     3541 aacggccggc ccgagggcta ctcggtctgc atcgtcagcg agggcgccat gccggccggc
     3601 ggcgagatgg tcttcatggg cggcgagaag gacatgttcg gccacgccaa gctcggcggc
     3661 atcggccaga acgtgagcaa caagatcaag gaactggcgc cggcgtacaa cggcggcaag
     3721 aagatcgaca tgatcagcat gaacctgagc tacctggtgc gcagcggtgc gccggacgcg
     3781 atcgactgca tcgtgccgac cgcctacggc aacctcgccg tcgacctcat cctcaagggc
     3841 cagaccggcc gcatggtcgc gctgcagggt ggccgctacg gctcggtgcc gatcgacgag
     3901 gtcgtctcga ccaagaaggt cgtcgacgtg ggccgctact acgacaccga gcgctatcgt
     3961 ccgaagtacg acgcactcga gctctcgccg atgatggtgc tgggcgggag ctgatgcgca
     4021 agcgcgtcct cctcgtcccc gacggcatgg cggacgagcc gcaggccgcc ctcggcggca
     4081 ggacgccgct ggaggcggcg tccacccccg ccatggactg cctcgcccgc ggcggcatct
     4141 gcggtctggc gcacaccgtc cccgacggca tgtcgcccgg cagcgacgtc gccaacctgg
     4201 ccgttctcgg gtacgacccg gccgccgtct tcagcgggcg cagcccgctg gaggcggcct
     4261 ccctcggcgt cgagctgggc gctgacgacg tttcctaccg gctcaacctc gtgaccgtcg
     4321 gcggcggcct catgaaggac aacaccgcgg gccacatcgg gagcgacgac gcccgtcgct
     4381 gtatcgaggc gctgcaggcc gagctcggca gcgacgcgta cgagttccac ctcggggtga
     4441 gctatcgcca cctgttcgtc tggcggggcg gggcgctggt gccgtgtacc ccgccgcacg
     4501 acatcctcga ccggccggtg gccgggcacc tgcccgggga ggcggccggc gcggccggcg
     4561 gcggctcgcc ggcggccgag ctcgccgcgc tccaggcgcg cgcgagcgag gtgctcgagc
     4621 gcgtccggcc cggcacgagc gcgtggttct ggggcgaggg cacggccccg cggatctcgc
     4681 cgttccgcga ccacttcggc ctcagcgccg ccgtggtcgg cgccgtcgac ctcgtgcgtg
     4741 gcatcggccg gctcgccggc ctcgacgtga tcgacgtgcc cggcgcgacc ggcggcctgg
     4801 acacggacta cggcgccaag gcgcgcgccg cgatcgcggc gctcgccgac cacgacctcg
     4861 tctgggtgca cgtggaatcg ccggacgagg ccggccacat gggcgacctg cgcgagaagg
     4921 tccgcgccat cgagcgcgtc gacgccgaga tcctcgcgcc gatcctggcg tccgccgggc
     4981 gcccggccgt gctcgtggtc cccgaccacc tcacgccgct gcgcacgcgc acgcacgcgg
     5041 ccggcccggt gccgttcgcc atcggcgact cgctgccggg ggcgggcggt ggcggcgcca
     5101 tggcctacgg ggagtcgcag gccgccatga ccggcctcgt cgtcgaggac ggcgcggcgc
     5161 tcatgcggct gttcctcgag gcgacggggt aagtacccgc ctgcggcgtc cgcacctcgc
     5221 gggcgccgaa ggcacgagcc gggaggcggt ggcgattgcg gcgatgagct cgtcccacga
     5281 acaaccagag cggcagccgg cgcgtgagcg cccggcggac gagcccgtcg tcaccaccac
     5341 gagcgccgaa cggtacgtga tgagcggggg gcgcacgtcg cggctcgcgg tcaaggagga
     5401 cgacctcttc ctctacacca acgagctcgg gcaggtgccg ggcacggaga actccgtgct
     5461 ggggctgtac taccgcgaca cgcgctacct gagcaggtac gtgctctcga tcgccggtcg
     5521 ccagccggtg ctcctcagcg cgagcggcga gcgcgggtac gcggcgacga tcgagctcac
     5581 caacctggag gcgcggaccg cggacgggcg cgtcctgcca caggccagcg tgcacgtgcg
     5641 ccgcacgcgc ttcgtctccg accggctgta cgagctcctc cgggtgcgca actaccacca
     5701 acgcgaggtg gacctggtcc tcgacctcga cttcgacgcc gacttcgccg acctcttcga
     5761 ggtgcgcggc agccggcggc gccggcgcgg ctcgcgtctc gcgccgcggg tgagcgacgg
     5821 cgtgctcacg ctctcgtatc tggggctcga tgacgtgacg cgccagacca tcgtgcgctt
     5881 ccacgaccgg ccggaatcgc tcaagcaggg gcgggcgcgg ttccgcctcc gcctcccgcc
     5941 gggcgagcgg gccgtcatcc gctacgacgt ccaggtggtc ggaccggagg ccccgggaga
     6001 ggacggcggc gagttcaacg cgcgcatcgg cgcgctccgc cacgagcacg agcgctgggc
     6061 atcgggcgcc accgacatct tcaccgacaa cgatcagctg aaccgggtgc tgcgccgggg
     6121 gcaggacgac ctgcggctgc tctccacggt gatcgacggc gagcgcgtcc cgctctcggg
     6181 gctgccgtgg ttcggggcgc ccttcggccg ggagatgacg ttcgtcgggc tcgagacgct
     6241 gttgctcgac ctgcgctggg cgcgctccgc ggtctcgttc ctcgcgcgcc gccagggcgt
     6301 gcaggacagc gccttccgcg aggagcagcc gggcaagatc atgcacgagc tgcggcgcgg
     6361 cgagcttgcc gccatccgcg ccgtgccgca cacgccgtac tacggctccg tggactccac
     6421 ggcactgtgg ctgctgctgg tcgccgagct cgcgatgtgg accggcgacc tggacgagtt
     6481 cgagctccgc gccgctgcgg tcgacgccgc gctggggtgg atcgacggcc cgggcgaccc
     6541 cgacggcgac gggttcgtgg agtacgagcg ccgctcccgc gtggggctgc gcaaccaggg
     6601 gtggcgcgac tcgttcgacg cggtgctgca cgaggacggc acgccggcca cgggtccgat
     6661 cgccctggcc gagacacagg gctacgtgta ctacgccaag cggcgcctcg cggccgtgtt
     6721 cggtcagctg ggagacgtgg agcgcgccga gcggctgtcg caggacgcgg cgcgcctcaa
     6781 gcgcgccttc aacgagcggt tctgggtcga ggaccagggc ttctacgcca tggcgctcga
     6841 cgggcagaag cgccaggtgc gctccgcgtc ctcgaccatc ggtcacgcgc tgtggtcgcg
     6901 gatcgtggcc gaggagcacg tcccggcagt cgtgaggcgg ctgatggcgc cggacatgtt
     6961 caccgggtgg ggcatccgca cgctcagcaa ggaagcgcgc tactacaacc cggtgagctt
     7021 ctacaacggc agcgtctggc cgttcgacac cgcgctcatc gcgaacggcc tcaagaagca
     7081 cggctacgtg caggaggcga accgcctcgc ctgggggctg gtggaggcgg cgtccgcaca
     7141 cgagtacgcc cgcttgccgg agatgttctg cggcttcacg cggcagacgg tcgaccgccc
     7201 ggtctccttc ccgatggcct gctcgccgga cgccaactcg gccgccgccc tgtacctcgt
     7261 cctgcagtcg atgctgggca tctacgccca ggccgaggag aacatcgtct acgtccacaa
     7321 cccggtgctg ccacgctggc tgggcgaggt gacgctcagc aacctgcgcg tcggccgcac
     7381 caccatgcgc ctccgtttcc gccgcgaggg cgggcagacg agcttctcgg tgctggacaa
     7441 gcaggggccg gggcgtatcg tggtcgtcga gtagtcgaat gctcacgatc gcccacatca
     7501 gcgacatcca cgccggctcg ccgtacttcg tcccgagcct catggaccgc gtcgtcgtgg
     7561 agctcaacga gctcgagccg gacgtcgtcc tgtgcaccgg ggacctcacc gagatgggct
     7621 tccgccagga ctacgtcacg gcgaaagagt acatggaccg catccaggcg ccgctggtcg
     7681 tcatcccggg caatcacgac gcgcgcaacg tcgggtacgt gcacttcgag gagctcatcg
     7741 gtccccgctg gcagacgctc tcgctgggcg gggtcaagat cgtggcgctc gactcctccg
     7801 agccggacct cgactacggg cagatcggca gggcccgtta cgacttcctg cgcgaggagt
     7861 tcggccgtcc ggccggcttc cgcatcttca tgctccacca ccacgtgctg cccatccccg
     7921 gcaccgggcg cgagcgcagc atggccctcg acgccggcga cctcctcgag gtgcttcagg
     7981 agtgcggcgt caacctggtg ctctccggcc acaagcacgt gccgtacgcc tggcgcctcg
     8041 agcacctctt cgtcgtcaac gccgggacgt gcagttctct ccgactgcgg ggcaagacca
     8101 aagcgtgcta caacctcgtc cgcatcgacg acgacagggt gcaggtgtcg cgcaagtacc
     8161 cgttccacgg tgaggagacg ctgatcgact tcgtgccctc caccgccgcg ttcgacaagt
     8221 acatctgccc tgcgccccgg gaggagagca gataggcacc ggccggccgc gcgccgtcgt
     8281 gctcgtcgac ggcgaacact acccacccgt ggtcgcggcc gcagtggcgc ggctcgaaga
     8341 cgcgtacgcg gtcgcgggag ggatcttcgc cggcggccgg gagaagctgc gcggcggcga
     8401 ggccggaggc ctcgccgcgc tcgccgccga gatcggggtg ccgcggctcg acgccgtgga
     8461 gccgcggtcc gcgtccgcgc acgaggtgct cgagggcgtg cgcgccgtgc tgcgcgcgct
     8521 gcgtgccgag gccctcgtcg acctctccga cgagccggtg gtgggctacc gcgagcggtt
     8581 cctgctcatg agcgcggcgc tggcggaggg cgccgcctat gtcacctccg acaccgaggt
     8641 acggccgcag gcgttcgcgc gactcgcggc cacgccgtcg ctggcggtca tcggcaccgg
     8701 caagcgcgtc ggcaagaccg cggtgagcgg ctggctggcg cggaggctcg acgccgcccg
     8761 gcgggcggac ggcggcgtcg tggtgctcgc catgggtcgc ggcggcccgc ccgagcccga
     8821 gctgatccag ggcggcgggc tcggccccgc cgagctgctc gccgcctcgc gcgccggccg
     8881 gcacgcggcc agcgactgct acgaggacgc ggtgctcgcc ggcgtgaccg cggtcgggtg
     8941 ccggcgctgc ggcggcggcc tcgccggcat gccgttcgac gacaacgtgc gcgaggcgct
     9001 gccgctgctc gacgggcgcg gcgcggcgct ggcggtgatc gagggcagcg gcgcggtggt
     9061 gccgcccgtc gcggccgatg cgacgctgtg cgtggcggga gcgagccagc cggccgacta
     9121 cgtcgccggc tacctcggca cgtaccggct gctgctcagc gacgccctcg tgctgacgca
     9181 gtgcgagacg ccgttcgcta gcccgggcga ggtcgccgcc atggccgccg cggccggggc
     9241 ggtcaggccc ggcctggagg ttgtgcggac ggtgttccgg ccgcggccgg cgcagtccgt
     9301 ccgtggccgc cgcgtggcct tcttcaccac cgcgcccgag gccgcggcgc cgcggctggc
     9361 ggcgtcgctg gccgaggagc acggcgcaga ggtcgtgctc gtgtcgtgcg acctcgccga
     9421 ccggcggcgg ctgcgggagg cggtggcgcg cgccgccgcc gaggcggacg tgttcctcac
     9481 cgagatcaag gcggcggcgg tggacgtggt cgccgagggc gccgccgccg ccggccgcga
     9541 gctggtcttc tgcgacaacg agccggtggc gctcgagggc gacctcgcgg cgacggtgga
     9601 ccggctcgcg gcgttcgcgg cggagcgctt cgcggcgcgc gcggacgcct cacaggagcg
     9661 gagcgcggac ggcgcaccgg gagactgacg gtgccggagc ctcacgacat ccccctgatc
     9721 gacgagggag accaggcgcc cttcagcaaa gggctcatgg cgcagacgct catggcgacc
     9781 ggtctggccc cggagagagc gtacaacgtg gcggccgccg tggagcggcg cctgcggcgc
     9841 cgcgggccgg cggccctgac cctcgccgac ctgcaggaga tcgctcgcga ccagctcggg
     9901 ccggagaagg gcgacgtcct catcgagcgc ttccgccagt ggcagaagct gcgccgcctg
     9961 gagacgccgc tgatcatcct catcggcggg gcgacggggg tgggcaagag caccctggcc
    10021 acccaattgg cgcaccgtct cggcatcacc cggctcatcg gcaccgacat ggtgcgccag
    10081 accatgcgcg cgttcttcgc cgccgagctc atgccggcga tccacacctc gtcgttcgac
    10141 gccgccgcgg ccgtccgcgt gcccgtgccg cgcgagaccg acctcagcaa gatggggttc
    10201 atcgagcaga ccaaggcggt ctcggtgggc atcgaggcgc tggtccagcg gggcatcgac
    10261 gaggcccagc ggatggtggt cgagggcgtg cacctcgtgc cgggcttcct cgaccgttcg
    10321 cgctgggggg aggcgatcgt gctcgagttc atgctcgcga tcagcgacaa ggagcgtcac
    10381 cgcggcaact tcacggtgcg cgagtgggag accgggggga tccggccgct gcgccgctac
    10441 atcgagcact tcgccgagat ccgccgcatc cagaagtaca tggtggggcg ggcgcaggcg
    10501 ctgggcgtgc ccgtgatcga cgggccgagc ctcgacgaca acctgagcga agtgctcggg
    10561 atgatcctcc gccgcgtgga ggaggagggc ggggagccgg agccggccgg ggagggcaag
    10621 gcgccgccgg cgtgacgcgg ggcttcagtt cggtcgccgg cgtccgatag actaagggta
    10681 tccccggtcc gcctcgtcct caggcacgcg cgcggaccac gtccacccac gcgagctgcc
    10741 gcgaggttac tgggaatctc ttgcgcggta tgacatactc tgcgagcggg gtccccacag
    10801 acccgtcttc caatcgcacc atccacttac tcgtacggca tctccccagc gcggcgtcgt
    10861 ggtacgtcgc tcagtcgatg ccaggagtcc cgggagccca tgagcactga agaacagaca
    10921 gatcagatga ccgttccgag cgaacccgcc gtggagaccg ccgtcgctcc gccggtggcg
    10981 gagaagcccg tcaaggacaa gccggccgag aagccggcgc gggcctccag ggccaaggcc
    11041 gcacccgccg cggccaaggc cgccggcgcg gcgtccaagg cggcggaggc tctcgctccc
    11101 gtgaaggcgg cggaggccgc cacggctccg gccgctcccg cgcccccggc cgccgaggcg
    11161 gcggctacgg cgcctgcggc ctacaagccc gccgaggcgg cggctccggc gcctgcggca
    11221 gacaagcccg ccgaggcgac ggctcaggcg cctgcggcag acaagcccgc cgaggccccg
    11281 gccgcggaga cgcagcccgc cgccccagcc gcggaggcca cggccgccgg ggctcccgcc
    11341 gccgcgcccg aggcgccggc cgccgacagg cccgccggcg acggcgagcg gccgcagcag
    11401 cagggcggcc agcagcaggg ccagcagcag cggcagggca agggcaagaa ccgccgcggg
    11461 cgcgacaagc gccagggcgg cggcggcggt ggcggcgacc agcagcgcga cgaccggccg
    11521 accatcccgt gcaccggcat cctggacgtg ctcccggacg gcttcgggtt cctgcgcacg
    11581 aacggcttca cccagggcga ccgcgacgtc tacgtgagcc tcagccagat ccggcgtttc
    11641 ggcctgcgcc gcggcgacct ggtcgccgga caggtgcgcg agcccaagga caacgagaag
    11701 tacaacgcgc tcctcaagat cgacagcgtc aacggcatcg acccggacac ggcccgcaag
    11761 cgccccgtgt tcgagcagct cacgccgctg ttccccgagg agcgcctgcg cctcgagacc
    11821 gacgagcacg acacggcggc gcgcatcatg gacctgatgt gccccatcgg caagggccag
    11881 cgcggcctga tcgtctcgcc gcccaaggcc ggcaagacca ccatcctcaa gaagctggcc
    11941 aactcgatcg ccgtcaacaa ccccgacgtc gacctcttcg tgctgctcgt cgacgagcgc
    12001 cccgaggaag tcaccgacat gcagcgctcc gtcatcggca ccgtcgtcgc cagcaccttc
    12061 gaccagccga gcgagaacca cgtgcaggtc accgagctgg tgctcgaccg cgtcaagcgc
    12121 ctcgtcgaga tgggccacga cgtcgtcgtg ctgctcgact ccatcacgcg catggcgcgc
    12181 gccttcaacc tcaacacccc ggccagcggc cgcatcctct ccggcggcgt ggacagcacg
    12241 gcgctgtacc cgcccaagaa gttcttcggg gccgcccgca acatcgagga gggcggctcg
    12301 ctcaccatcc tcgccacggc gctggtcgat accggctcgc gcatggacga agtgatcttc
    12361 gaggagttca agggcaccgg caacatggag atccggctcg accgcaagct cgccgacaag
//
LOCUS       DASUMX010000098         3806 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_26587, whole genome shotgun sequence.
ACCESSION   DASUMX010000098 DASUMX010000000
VERSION     DASUMX010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3806
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26587"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="VFZ86_04815"
     CDS             complement(<1..218)
                     /locus_tag="VFZ86_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013132975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaB family protein"
                     /protein_id="HEX5641646.1"
                     /translation="MPSTIARSDEKAQRTWKETHDSAVEQYGEGERAHRTAFASLKHT
                     YEKAGDRWVPKDEKGASDPRSEMSTEDAR"
     gene            complement(280..2655)
                     /locus_tag="VFZ86_04820"
     CDS             complement(280..2655)
                     /locus_tag="VFZ86_04820"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX5641647.1"
                     /translation="METEDPTPDPQPPRNPVARAFAWVVAGPAAPLIPLAWIAAAVLA
                     VLYLPALAPEAGSLADYTPRDTPAIATEARSARIFGQPISRVAVVQRDPAGLSPAVQR
                     ETVAAAVDLAQQPRGGLLGALPLVNDPRIWPQGDEQATTAVTYLYFAPETSWREQVDL
                     ARAYASQRLTHADASVVGVTGSVPARVAQEDAIAERLGAVEIATVILVALILGVTFGS
                     LLAPLVTLLAAGVAYVIGSRLVALAGRELGLTPPSEIDPLVVVLLLGIVADYAIFYLS
                     GARERLGRGGGRGEAVRDTTAQLTPIILTAGLMVAAGTAALLAATQATFRSFGPGLAI
                     SVLAGLVVSVTFIPAAMATFGRALYWPRGLRASGSDDRERPGWLARQRERVGHLATHR
                     AAALGTVVVCVALLLVAASGLRWIGLGLDFISGLPSSSEPRQAAQAATAGFAPGILSP
                     TEVLLQKPGVGTQAEQLTSLEEAIARQPGVAAVVGPREQPPDPRIPFAVAPDMNAARY
                     YVVFSTDPLGSSGIDTLESLQAAMPGLVREAGLTDVRVGYAGDTAIAAFTVGRTIGDL
                     GRVGLAVIVIDIILLALFLRSLAAPLYLVAASVLALAAALGATTYFFRLLSGPDGLTY
                     YVPFAASVLLISLGSDYTIFVVGRIWEERKVRPLRGAIETAVPKARSAVSVAAVTLAL
                     SFSMLAIVDLWSFRQLAFLLAAGVLIDAFFVRSLLVPALVALFGRTWTGEAAERGAEA
                     SAGGGPQGPPASAPKAPSAQSTQSTPNPSRHPEPGRLEPGHIDGSRRRGAA"
     gene            2809..3252
                     /locus_tag="VFZ86_04825"
     CDS             2809..3252
                     /locus_tag="VFZ86_04825"
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX5641648.1"
                     /translation="MSTMYTAPVIRNRFSQHRFPAALKELMEERNLSYRQLAYKTKLS
                     AGYLNHLTKGTRPVPADQVIAVIAEALHVAPDFFLEYRLRQVACVLDQSPALADKLYG
                     ILLRDLPVPDDIAESLKRPRDEGNGGDNGGNGGGGGAGPRLSGGV"
     gene            complement(3322..3687)
                     /locus_tag="VFZ86_04830"
     CDS             complement(3322..3687)
                     /locus_tag="VFZ86_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641649.1"
                     /translation="MPHRNRIAQLLLVLYAAGSVVVAMPLLFAFGNAGDLADTTSGKV
                     LAAALLAMAFGALLAARDPWENRVLIPVLIAFTAMSALAIVYRLAFEGHTDDPAWMVL
                     PLAVAAPVLLALFYPRRPA"
BASE COUNT          554 a         1369 c         1400 g          483 t
ORIGIN      
        1 cgggcgtcct cggtcgacat ctccgagcgc ggatcggagg cgcccttctc gtccttgggc
       61 acccagcggt cgcccgcctt ctcgtaggtg tgcttgaggg aggcgaaggc ggtccggtgg
      121 gcccgctcgc cttcgccgta ctgctcgacg gcgctgtcgt gtgtctcctt ccaggtgcgc
      181 tgcgccttct cgtccgagcg cgcgatggtg gagggcatgt cgtcgcgcgc cggcgcgcgc
      241 ttgcgcttct tctcgtgctt gggcacggtg atcactcctt caggcggctc cgcgccgcct
      301 cgatccgtcg atgtgtccgg gttccagacg gccgggctca gggtgtcggg atgggttcgg
      361 cgtgctctgg gtgctctgtg cgctcggtgc tttcggcgcg ctcgccggcg gcccctgtgg
      421 gccacctccg gccgaagcct cggcacctcg ctccgcggcc tcgcccgtcc acgtgcgccc
      481 gaagagggcg accaacgcag gcaccagcag cgagcgcacg aagaaggcgt cgatcagcac
      541 cccggcggcc aggaggaagg cgagctggcg gaacgaccac aggtcgacga tggccagcat
      601 ggagaagctg agcgcgagcg tcacggccgc caccgagacg gcgctgcgtg ctttgggcac
      661 cgccgtctcg atcgcgccgc gcagggggcg caccttgcgc tcctcccaga tgcgcccgac
      721 gacgaagata gtatagtcgc tgcccagtga gatcagcagc accgaggccg cgaacggcac
      781 gtagtaggtg agcccgtcgg ggccggacag gagccggaag aagtaggtgg tggcgccgag
      841 ggcggccgcc agcgccagga cgctggccgc gaccagatac agcggcgcgg ccaggctgcg
      901 caggaacaac gccagcagga tgatgtcgat cacgatcacg gcgaggccga cgcggcccag
      961 gtcgccgatc gtgcggccga cggtgaaggc ggcgatcgcc gtgtcgcccg cgtatcccac
     1021 acgcacgtcc gtgagcccgg cctcacgcac gaggccgggc atcgccgcct gcagagattc
     1081 cagcgtgtcg atgccgctgc tgccgagggg gtcggtggag aacaccacgt agtatcgcgc
     1141 cgcgttcatg tcgggcgcca cggcgaacgg gatgcgcggg tctggcggct gctcccgcgg
     1201 gcccacgacg gcggcgacgc ccggctgccg cgcgatcgcc tcctcgagtg aggtgagctg
     1261 ctccgcctgc gtccccaccc cgggcttctg caggaggacc tcggtagggg agaggatgcc
     1321 cggggcgaag ccggccgtgg ccgcctgggc ggcctgccgc ggttcgctcg aggacggcag
     1381 gccgctgatg aagtccaggc cgaggccgat ccagcgcagg ccggaggccg cgaccagcag
     1441 gagcgcgacg cagacgacga cggtgcccag cgccgcggcg cggtgggtgg ccagatggcc
     1501 gacccgctct cgctgccgcg ccagccagcc ggggcgctcg cggtcgtcgg acccggaagc
     1561 gcggaggccg cgcggccagt agagggcgcg gccgaaggtg gccatcgccg cggggatgaa
     1621 ggtgacgctg acgacgagcc cggccaggac gctgatcgcc aggccgggcc cgaacgagcg
     1681 gaacgtcgcc tgggtcgcgg ccagcagggc cgccgtgccg gcggccacca tcagcccggc
     1741 ggtgaggatg atcggcgtga gctgcgccgt cgtgtcccgc acggcctcgc cgcgtccgcc
     1801 gccgcgtccg aggcgttccc gggctccgga caggtagaag atcgcgtagt cggcgacgat
     1861 ccccagcagc aggaccacca ccagggggtc gatctccgag ggcggggtga gcccgagctc
     1921 gcgcccggcg agcgccacga gccgcgagcc gatgacgtag gccacgccgg ccgcgagcag
     1981 cgtcaccagg ggcgccagga gcgacccgaa ggtgacgccc aggatcagcg ccacgaggat
     2041 gacggtcgcg atctcgacgg cgccgaggcg ctcggcgatc gcgtcctcct gcgcgacgcg
     2101 cgccggcacc gagcccgtca cgcccaccac ggacgcgtcc gcatgcgtca ggcgctggga
     2161 cgcgtaggcg cgcgccaggt cgacctgctc ccgccacgac gtctccggcg cgaagtacag
     2221 ataggtgacc gccgtggtcg cctgctcgtc cccctgcggc cagatccgcg ggtcgttcac
     2281 gagcggcagc gccccgagca gcccccctcg cggctgctgc gccaggtcca cggccgccgc
     2341 gaccgtctcg cgctggacgg cgggggagag gccggccggg tcccgctgca ccacggcgac
     2401 ccggctgatc ggctggccga agatgcgcgc cgagcgcgcc tccgtcgcga tcgccggcgt
     2461 gtcccgcggc gtgtagtcgg ccaggctgcc cgcctcgggc gccagcgccg gcaggtagag
     2521 caccgcgagc accgccgcgg cgatccacgc gagagggatc agcggcgccg cgggccccgc
     2581 gacgacccac gcgaacgctc tggccacggg gttgcgcggg ggctgggggt ctggtgtcgg
     2641 gtcctcggtc tccacgcccc tttcgttccc gaggctcgca cccgcaatcc ggcgggccgt
     2701 aagggcctgc tcggacgggc cgccggcgcc acgcgtaagc agtcctgaca acgccgtaag
     2761 tgttttcact caagtatccg ggccttccgg gcgatatgtg ctaccggaat gagcaccatg
     2821 tacacagcac cggtcatcag gaacaggttc tcgcagcacc gcttccccgc ggctctcaag
     2881 gagctcatgg aggagcgcaa cctcagctac cgccagctcg cctacaagac caagctgtcg
     2941 gcggggtacc tgaaccacct gaccaagggg acgcgccccg tgccggccga ccaggtgatc
     3001 gcggtgatcg ccgaggccct gcacgtggcg cccgacttct tcctggagta ccgcctgcgc
     3061 caggtggcct gcgtgctcga ccagtcgccg gccctcgccg acaagctgta cggcatcctg
     3121 ctgcgcgacc ttcccgtgcc cgacgacatc gccgagagcc tcaagcgccc gcgcgacgag
     3181 ggcaacggcg gggacaacgg cggcaatggg ggcggcgggg gcgccggccc cagactcagc
     3241 ggcggcgtct gaccgcttcg ctctctcgct gcgtgcatcg cgacggccgg ccccgagccg
     3301 gccgttctgc gtacgcgtcc atcacgcggg ccgccgcgga tagaagagcg cgagcagcac
     3361 cggcgcggcc accgcaagcg gaaggaccat ccacgccgga tcgtccgtgt gcccttcgaa
     3421 ggcgagccgg tacacgatgg ccagcgccga catggccgtg aacgcgatca gcacggggat
     3481 cagcacgcgg ttctcccacg gatcgcgggc cgccagcaga gccccgaacg ccatcgccag
     3541 cagggccgcg gcgaggacct tacccgaggt cgtgtccgcg aggtcgccgg cattgccgaa
     3601 ggcgaacagc agcggcatcg cgacgacgac actgccggcg gcgtagagga cgagcaggag
     3661 ctgtgcgatg cgattcctgt ggggcactgt gtctctcggt ccgcctccgg aacgggatca
     3721 gtatggtcgg tggcgccgcg cttcgccagc cggacgccgg cctggcccgg tgcgcgcacg
     3781 cgtcctgctc acgcgcgaga cgggcg
//
LOCUS       DASUMX010000099         5309 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1176369, whole genome shotgun sequence.
ACCESSION   DASUMX010000099 DASUMX010000000
VERSION     DASUMX010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 5309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5309
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1176369"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="VFZ86_04835"
     CDS             complement(<1..222)
                     /locus_tag="VFZ86_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyneurosporene dehydrogenase"
                     /protein_id="HEX5641650.1"
                     /translation="MTTQPARQADRPADYARLGLERRLIKPWEDGIRIDTKAPNLEWW
                     YCDADLDDGTGLCMIFATKDGTRPHQPLGP"
     gene            400..1017
                     /locus_tag="VFZ86_04840"
     CDS             400..1017
                     /locus_tag="VFZ86_04840"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5641651.1"
                     /translation="MKPLDEKADAGRRRPRRSREQVSQALIDATASLLADHASGRVTV
                     REIAAAADVNPTFVHRYFGSKRNLMRAAIERADDHVIAQIETMPDVVKGGPAVFHAAL
                     QEREIVAALARASLDGVLDDLPSGRPAVGALLERFLCEVESGQTRGPHDPRVIVACLA
                     SATMGYALFGPFIRRGVGLDEEPDTSVEAAMVAVLQKVARLAFSE"
     gene            1060..1233
                     /locus_tag="VFZ86_04845"
     CDS             1060..1233
                     /locus_tag="VFZ86_04845"
                     /inference="COORDINATES: protein motif:HMM:NF020135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HEX5641652.1"
                     /translation="MQRDVEAGRRHEGDVFGGAIARLDERHGLDTPSTRQVHARLSRP
                     PSPRRFPGAGPVK"
     gene            1283..3334
                     /locus_tag="VFZ86_04850"
     CDS             1283..3334
                     /locus_tag="VFZ86_04850"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEX5641653.1"
                     /translation="MTARRLGRTRVKMGVLAGALAAVLLAAALMTLLLPTPAGAEQPF
                     RVGSQIEDRAAVLGGREDDVWAAIEELQDGEQVQLWVTFVDTFSGVAAQDWANETAVQ
                     SDLGLRDVLLAVAVQDRAYAYSVEQDFPLSQEQLDGVMTASVEPRLSDDDWAGAAIGA
                     ADGIGEALRGEAASASSGGGSSSDGGGSSFPWLLAIGAVALVAVIAWFFVRARSRRAA
                     EGVPATGGADVLPLDELRRRANAELVAADDAVKTSAQELDFAVAEFGEEPAAPFRQAL
                     EEARGDVAEAFRLRNELRDDDEGRQREILTAILQHTDAANTVLDAQADRFDSLRDLER
                     NAPEVLAKLEGQLTELEARRPQVVDELAALAAGYAPAALLAVRRNPDEAQARIDFARE
                     QIRLGREDLAAERRGEAAVSALAAQEATGQAQTLLDAVGRLRQELQEAGARIADAVAE
                     TRRDIAEAQALGAGERLAPRIAGAEAALAAAGEAAAAAGGRDPLTALRHLEEADASLE
                     QALVGVRDEQARVARAAEVLDRTLLAARSEVASAEDFITTHRGAVGSGPRTLLAGAQR
                     SLDQAMALASSDPVTAAHHAAQAQQQAARALSDASDEAQQGAGMGMPGMPGMPGAGRS
                     GGDIGGMIIGGILAGMLGGGGFGGGSGGGRSGGFGGGGFGPPSFGGGGTRMRRGGGGR
                     F"
     gene            3359..4063
                     /locus_tag="VFZ86_04855"
     CDS             3359..4063
                     /locus_tag="VFZ86_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013601640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HEX5641654.1"
                     /translation="MVQQSIFGRIAQLARANINSIIDSAEDPEKMLDQMIRDYTSNIS
                     EAEEAVAQTIGNLRMMEEDQREASEAADDWGRKAAAAAAKAGELKAGNPDEAQRFENL
                     ARVALKRQIGFEQQQKDLAPTIAQQNEVVEKLKSGLNSMRGKLEELKMKRDELVARSK
                     TAEAQTKVHDALKSVNITDPTSELSRFEEKIRREEARVVGQAELAASSLDAQFEGLED
                     VAEDAEVEARLADLQK"
     gene            complement(4217..>5309)
                     /locus_tag="VFZ86_04860"
     CDS             complement(4217..>5309)
                     /locus_tag="VFZ86_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016883316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HEX5641655.1"
                     /translation="NGGTSDPCVISWPAGMKTRGEIREQYHHAIDIVPTILDCLGVAA
                     PETIKGNVQSDFDGLSMRYSFDDASAEGTRATQFYSMLGQRAIWHDGWKAVTTHPPLS
                     GWSDFNDDDWELYHTDVDRAELHDLAAEMPDKVRELVNLWFAEAGRNAAFPLDDRLVL
                     EIGLAERPQLAAPRDKYIYYPGIADVPESQSVNIRNRSYSLGALVDIPAPGAEGVLFA
                     HGARFGGHALYVKDARLHYVYSFVGVMEQKIDATEDVPTGEDLILAATFEKDGEDPPG
                     VATGVLSLYYGERKVGEGRIKTQPGQFSLGGEGLCVGRDSGAPVTDDYPGEAPYRFTG
                     GTIKQVRVNVSGEPYLNLEREAVAMLMRE"
BASE COUNT          788 a         1835 c         1951 g          735 t
ORIGIN      
        1 cggcccgaga ggctggtgcg ggcgggtgcc gtccttcgtc gcgaagatca tgcacaagcc
       61 ggtgccgtcg tccaggtcag cgtcgcagta ccaccactcc aggttgggcg ctttggtgtc
      121 gatgcggatg ccgtcttccc acggcttgat gaggcgtctc tcgaggccga ggcgcgcgta
      181 gtccgcgggc ctgtcggcct ggcgggccgg ttgggtggtc atcgggactc cctcctcgtc
      241 gcgttggcgt cgaccgaccc tcgccacttc ccgctcacct cgcacgaccg ccgcgggcct
      301 cggcggggac gacaaaagta tacacgcgac tacattatgc ctaacggtgc tacgatgcca
      361 gggcagggac cgttctgggg acccacgagg gagcagacga tgaaaccttt ggacgagaag
      421 gccgacgccg ggcgaaggag accacggcgt tcacgcgagc aggtcagtca ggcgctgatc
      481 gatgccacgg cttcgctcct ggccgatcac gcatctggcc gcgtcacggt ccgcgagatc
      541 gccgccgccg ccgacgtcaa tcccacgttc gtgcaccgct acttcggctc caagcgcaac
      601 ctgatgcgcg cggctatcga gcgcgcggac gaccacgtca tcgcgcagat cgagacgatg
      661 ccggatgtgg tcaagggcgg gccggccgtc ttccacgcgg cgctgcagga gagggagatc
      721 gtcgccgcgc tcgcgcgagc gagcctcgac ggcgtcctcg acgacctgcc ctccggccgg
      781 cccgcggtcg gcgcgctgct ggagcggttc ttgtgcgagg tggagagcgg tcagacccgc
      841 ggtccccacg acccacgcgt catcgtcgcg tgcctcgctt ccgccaccat gggctacgcc
      901 ctgttcggtc cgttcatccg ccgcggcgta ggtctggacg aggaacccga cacaagtgtg
      961 gaggccgcca tggtggctgt gctgcagaag gtcgcgaggc tggccttcag cgagtgacgg
     1021 agccaaggcg cgggcgtttc cttcagggcc aggacgtcgc tgcagcgcga cgtcgaggcc
     1081 gggcgccggc acgagggtga cgtgttcggc ggggccatcg ctcgcctcga tgagcgacac
     1141 ggactggaca cgccgagcac gcggcaggtc cacgcacgcc tctcgcggcc tccatcgccg
     1201 agacggtttc ccggcgccgg tccagtgaaa tgaagcgaca agggagcacc cagacgaccg
     1261 acccgggatc aaggaggcgc acgtgaccgc tcggcggctc ggacgtacgc gcgtcaagat
     1321 gggcgtgctc gcgggcgcgc tggccgccgt actgctcgcg gcggcgttga tgacgttgct
     1381 cctgccgacg ccggcaggcg ccgagcagcc gttccgggtg ggcagccaga tcgaggaccg
     1441 cgcggccgtg ctcggcggcc gggaagatga tgtctgggcc gccatcgagg agctgcagga
     1501 cggcgagcag gtgcagctct gggtgacgtt cgtcgacacc ttctcgggcg tcgccgccca
     1561 ggactgggcc aacgagaccg ccgtccaaag cgacctcggc ctccgtgacg tactgctcgc
     1621 ggtcgcggtg caggaccgcg cctacgcgta ctcggtcgaa caggacttcc ccctcagcca
     1681 ggagcagctc gacggcgtca tgaccgcatc cgtcgagccg cgtctctcgg acgacgattg
     1741 ggcgggcgcc gccatcggcg ccgccgacgg gatcggtgag gccctgcgcg gtgaggccgc
     1801 ttcggcatcg tccggcggcg gcagcagcag cgacggcggc gggtcctcct tcccgtggct
     1861 gctcgcgatc ggcgccgtcg ccctcgtcgc ggtgatcgcg tggttcttcg tgcgggcgcg
     1921 gtcgcggcgg gccgccgaag gcgtgcccgc caccgggggc gccgacgtgc tgccgctgga
     1981 cgagctacgg cgccgtgcca acgccgagct cgtcgcggcc gacgacgccg tgaagacgag
     2041 cgcccaggag ctggacttcg cggtcgccga gttcggcgaa gagccggcgg cgcccttcag
     2101 gcaggcgctg gaggaggcgc gcggcgacgt cgcggaggcg ttcaggctgc gcaacgaact
     2161 gcgagacgac gacgagggac ggcagcggga gatcctcacg gccatcctgc agcacacgga
     2221 cgcggccaac accgtgctcg acgcccaggc ggaccgcttc gacagcctgc gggacctcga
     2281 gcgcaacgcg ccggaggtgc tcgcgaagct ggaaggacag ctcacggagc tcgaggcgcg
     2341 gcggccgcag gtcgtggacg agctcgccgc gctcgccgcc ggctacgcgc cggcggcgct
     2401 gctcgcggtg cggcgcaacc cggacgaggc ccaggcgcgc atcgacttcg cgcgtgagca
     2461 gatccgcctg ggccgcgagg acctggcggc ggagcggcgc ggcgaggcgg ccgtctctgc
     2521 cctcgccgct caggaggcga ccgggcaggc gcagacgctg ctcgacgcgg tcgggcgcct
     2581 gcgccaggag ttgcaggagg ccggcgcgcg catcgccgac gccgtcgccg agacgcggcg
     2641 ggacatcgcc gaggcgcagg cactcggcgc cggagagcgg ctggcgccgc gcatcgccgg
     2701 cgccgaggcg gcgctcgccg cggccggcga ggcggccgcg gcggcgggag ggcgcgaccc
     2761 gctgacggcg cttcgccacc tcgaggaggc ggatgcgtcc ctcgagcaag cgctcgtcgg
     2821 cgtccgcgac gagcaggcgc gcgtggcgag ggccgccgag gtgctcgatc gcacgctgct
     2881 cgccgcgcgc tcggaggtcg cctcggccga ggacttcatc acgacgcatc gcggcgccgt
     2941 cggcagcggc ccgcgcacgc tcctggcggg ggcgcaacgc tcgctcgacc aggcgatggc
     3001 cctcgcatcc tcagatccgg tgacggccgc gcaccacgcc gcccaggcac agcaacaggc
     3061 tgcacgcgcg ctgagtgacg cttccgacga ggcgcagcag ggcgccggca tgggcatgcc
     3121 cggcatgccc ggcatgcccg gcgccggccg ctccggcgga gacatcggcg gcatgatcat
     3181 cggcggcatc ctcgccggca tgctgggcgg cggaggcttc ggcggaggct ccggcggcgg
     3241 gcgttcggga ggcttcggcg gcgggggctt cgggccgccc tccttcggcg gcggcggcac
     3301 gcgcatgcgc cggggcggcg gcggacggtt ctagagacac acaggcaggg aggtactaat
     3361 ggtccagcag tccatcttcg ggcgcatcgc gcagctcgcg cgtgccaaca tcaactcgat
     3421 catcgactcg gcggaagacc ccgagaagat gctcgaccag atgatccgcg actacaccag
     3481 caacatctcg gaggctgaag aagccgtcgc gcagacgatc ggcaatctgc gcatgatgga
     3541 ggaggaccag cgcgaggcga gtgaggcggc ggacgactgg ggccgcaagg ccgctgccgc
     3601 cgccgccaag gccggtgagc tcaaggccgg caacccggac gaggcgcagc gcttcgagaa
     3661 cctggcgcgc gtggccttga agcgccagat cggcttcgag cagcagcaga aggatctggc
     3721 tcccacgatc gcgcagcaga acgaggtggt ggagaagctc aagagcgggc tcaactccat
     3781 gcggggcaag ctcgaagagc tgaagatgaa gcgtgacgaa ctggtggcgc gctccaagac
     3841 cgccgaggcg cagacgaagg tgcacgatgc gctcaagagc gtgaacatca ccgacccgac
     3901 gagcgaactg agccgcttcg aggagaagat ccgccgcgag gaagcccgcg tcgtcggcca
     3961 agcggagctg gcggcgtcgt cactcgacgc gcagttcgag ggcctcgagg acgtcgccga
     4021 ggacgcggag gtcgaggcgc gcctggccga tctgcagaag tagtccggcc aggcgtgacg
     4081 acgagggggc ggctgccgtc ctggcagcct ccccctcatc ccgctcgcgc gcgagcgcgc
     4141 ctgctctccc gcgcgcccgc gcgtcagcag gcatgtcgcg tcacgcgtcg ccgcgttcgc
     4201 gcaccgtgcc ggcccttcat tcccgcatga gcatcgccac cgcctcgcgc tccaggttga
     4261 gatagggctc gccgctcacg ttgaccctca cctgcttgat ggtgccgccg gtgaaccggt
     4321 acggggcctc gccggggtag tcgtcggtga ccggggcgcc gctgtcgcgc ccgacgcaga
     4381 gcccttcgcc ccccaatgag aactgcccgg gctgcgtctt gatccgcccc tcgcccacct
     4441 tcctttcgcc gtagtagagg gacagcacac ccgtggcgac gcccggcggg tcctcaccgt
     4501 ccttctcgaa ggtggcggcc aggatcaggt cctcgccggt cgggacgtct tccgtcgcgt
     4561 cgatcttctg ctccatcacg ccgacgaagc tgtagacgta gtgcagcctg gcgtccttca
     4621 cgtagagggc gtgcccaccg aagcgtgcgc cgtgggcgaa cagcacgccc tcggcgcccg
     4681 gcgccgggat gtcgaccagc gcgccgaggg agtaggagcg gttgcggatg ttgacggact
     4741 ggctctcggg gacgtccgcg atgcccgggt agtagatgta cttgtcgcgc ggcgcggcga
     4801 gctgcggccg ctccgcgagc ccgatctcga gcaccaggcg gtcgtcgagc gggaacgccg
     4861 cgttcctgcc ggcctccgcg aaccagaggt tcacgagctc gcgcaccttg tcgggcatct
     4921 ccgcggccag gtcgtgcagc tccgcgcggt cgacgtcggt gtggtagagc tcccagtcgt
     4981 cgtcgttgaa atcgctccac ccgctgagcg gcggatgggt cgtgacggcc ttccacccgt
     5041 cgtgccagat cgcgcgctgg ccgagcatcg agtagaactg cgtcgcgcgc gtcccctcag
     5101 cggaggcgtc gtcgaagctg taccgcatgc tgaggccgtc gaagtcgctc tgtacgttcc
     5161 ccttgatggt ttcgggagcc gctacaccga ggcagtcgag gatcgtgggg acgatgtcga
     5221 tcgcgtggtg gtactgctct ctgatctcgc ccctggtctt catccccgcc ggccaggaga
     5281 tgacgcatgg gtcggaggtg ccgccgttg
//
LOCUS       DASUMX010000100         5808 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1463841, whole genome shotgun sequence.
ACCESSION   DASUMX010000100 DASUMX010000000
VERSION     DASUMX010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 5808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5808
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1463841"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="VFZ86_04865"
     CDS             complement(<1..443)
                     /locus_tag="VFZ86_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020677938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5641656.1"
                     /translation="MTAATMTTHETDTTVLGAPAGRPEDDRAARFIDVRGLEKTFAGD
                     GAPVHALRGLDLTAHEGTFLGVMGQSGSGKSTFLAILGGLSHPSTGTVAVDGIDLYAL
                     RGERLADFRREYLGFVFQSFNLVPYLTALENVTLPLAVKKMPAAV"
     gene            complement(473..1630)
                     /locus_tag="VFZ86_04870"
     CDS             complement(473..1630)
                     /locus_tag="VFZ86_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020677939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5641657.1"
                     /translation="MTLRSIAFANLRRRKARAAFLIAGLLIGVATVVALLSLARSMAG
                     EAEANLESYGANIVITPKAEDVALTYGGIDVGGVSVGDQGLDEADLERVRSIPARGSI
                     AVVAPELVGAVEVKGRRALLMGVDPAAQFELKRWWSIGAGRPPQNERELIAGARAAEV
                     LQLDMGDYVRIRGERFTVTGVLWPTGGQDDELLVARLGDVQRVLGRPGELTLIEVAAT
                     YSGASVDQVVRQLSAALPQADVSTVREAVASRLHAVDQFKGFSLVIVGIVVAIEILVV
                     FLTMMGSVSERTTEIGVFRAIGFRREQITRLVLIEAVAAGLLAGVLGYAAGMAVTYAV
                     LPWLAEGAPVAWTPLLGVAAVAAAALTGALAALYPALRAGKLDPTEALRAL"
     gene            complement(1644..2804)
                     /locus_tag="VFZ86_04875"
     CDS             complement(1644..2804)
                     /locus_tag="VFZ86_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020677939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX5641658.1"
                     /translation="MRLHTVALGNLRRRSSRAAFLVAGLLVGIATVVTLLTVSRALTV
                     QAQNQLETYGANIVVAPRTDDLSLSYGGVALGGVRLQARDLREADLSRISTIPNSKNV
                     AIVAPELLGAVEAKGRQTLLLGVRPEDEFELKKWWKLDGRPPAAADELVAGSSAARRL
                     DLGIGDTVALDGRDFRVTGVLRPTGSQDDELLVTQLGAAQALLGKPGKVTLVQVAALC
                     SDCPVEEIAVQLGDVLPGTKVTAMQQVTRNRMHALDTFRTFSYVIAAIIIAIEALVVF
                     VTMMGSVNARTREIGIFRALGFRRSHVTLLVLMEAAVASVVAGVLGYLVGMGASYLLL
                     PFFAEGLNVTVSWAPALAAGAVLLAVLIGSLGSLYPAMRASRLDPTVALRAI"
     gene            complement(2854..3432)
                     /locus_tag="VFZ86_04880"
     CDS             complement(2854..3432)
                     /locus_tag="VFZ86_04880"
                     /inference="COORDINATES: protein motif:HMM:NF021587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2318 domain-containing protein"
                     /protein_id="HEX5641659.1"
                     /translation="MSGKKSGRTSTTDRRGVTHKEDVRAGKRAQFEQPKSRTGLHLAL
                     AGVALVAVIAVALVVVMDRGDQGAPAAAAPVAAGGADVTIPVADLADGQARYYAYDAG
                     GVEVKYFVMKSADGEYRAAFDACDVCFANKKGYTQQGDVMVCNNCGQQFDSTQINEVR
                     GGCNPSPIERSVDGQNLVLKTADLQTGVKYFQ"
     gene            3618..4298
                     /locus_tag="VFZ86_04885"
     CDS             3618..4298
                     /locus_tag="VFZ86_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010493233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5641660.1"
                     /translation="MVVIDDEESVRDVVRAYLEKDGFRVFVAENGRDGLALAERHQPD
                     LIVLDLMLPDVSGEEICAEIRDRSDVPIVMLTAKASEEERVGGLVAGADDYLVKPFSP
                     RELVARVRAVLRRTGGVETPLVEVLRFDDGRLEIDTVKRAVRRDGDPVELTPNEYKLL
                     VALARYPGRAYSRFELVSRVQGYDYEGYERTIDVHVKNLRKKLEPDPGRPRYIETVTG
                     VGYRLAKL"
     gene            4307..5641
                     /locus_tag="VFZ86_04890"
     CDS             4307..5641
                     /locus_tag="VFZ86_04890"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5641661.1"
                     /translation="MRGGLRTRLVIAFVAVALLGALVTTVYSSLSLTARLETSAEARL
                     QNSALHFGEVAAVVSDGSGWSRQSIETLHHLAQIETLAARLYDVRGELVFEHPASGPV
                     EPGAAASSPVMAGERRIGRVDISRDDGRLFTEEEIRLRSQLLRTNLIAGVTSAAIALA
                     VALYLAVTLSRPLRHIRAGAEAMGAGDLDTRVPESGDDEIAAVAAALNRLAETLQQEE
                     ALRKESVADLAHELRTPVMGVLARVEAAQDGVLEDDAANLSAMHDEALRLSRLLDDLS
                     ALAEAERPGLLLDAEPVDLAAIARAQTTAFAAAFAEKDIALETDLLPAPVSGEPKRLE
                     QVVVNLLSNALRYTDAGGRVLVTVRPDGQAALLEVSDTGIGIAEEDLPRVFTRFWRGE
                     KSRSRDTGGAGIGLAIVRELVQAHGGEVGVESAPARGSLFRVTLPLSPHASR"
BASE COUNT          827 a         2080 c         2094 g          807 t
ORIGIN      
        1 ttcacggcgg ccggcatctt cttcaccgcc aacggcagcg tgacgttctc gagcgccgtc
       61 aggtagggca cgaggttgaa ggactggaac acgaaaccga ggtactcgcg gcggaagtcc
      121 gcgagccgct cgccgcgaag cgcgtacagg tcgatgccgt ccaccgccac ggtgccggtg
      181 ctcgggtggc tgaggccgcc caggatggcg aggaaggtgc tcttgccgga gccggactgg
      241 cccatgacgc cgagaaacgt accctcgtgc gccgtcagat ccagcccgcg cagcgcgtgc
      301 acgggcgcgc cgtcgccggc gaacgtcttc tccaggccgc gcacgtcgat gaagcgcgcg
      361 gctcggtcat cctccggccg gccggcgggg gcgccgagca cggtcgtgtc cgtctcgtgc
      421 gtcgtcatgg tggcagcagt cattccctct ctcctgatac ggacgcccgc gctcacagcg
      481 cgcgcagcgc ctcggtgggg tcgagcttgc cggcgcggag cgccggatag agggcggcga
      541 gggcgccggt gagcgccgcc gccgccacgg cggccacgcc gagcagcggc gtccacgcca
      601 cgggcgcgcc ctcggcgagc cacggcaaca cggcgtaggt caccgccatg cccgccgcgt
      661 agccgaggac gccggcgagc aggccggcgg cgaccgcctc gatgagcacg agccgcgtga
      721 tctgctctct ccggaagccg atggcgcgga acacgccgat ctccgtggtg cgctcgctca
      781 cggagcccat catggtgagg aacaccacga ggatctcgat cgccaccacg atgccgacga
      841 tgacgaggct gaaccccttg aactggtcca ccgcgtgcag gcggctcgcc accgcctcgc
      901 ggaccgtgga cacgtcggcc tgcgggaggg ccgcggagag ctggcgcacc acctggtcga
      961 cggaggcgcc ggagtacgtg gccgcgacct cgatgagcgt gagctctccc ggacgtccga
     1021 gcacccgctg cacgtccccg agccgggcga cgagcagctc gtcgtcctgc ccgcccgtcg
     1081 gccagaggac gcccgtgacg gtgaagcgct cgccacggat gcgcacgtag tcgcccatgt
     1141 ccagctgcag cacttcggct gccctggcgc cggcgatgag ctcgcgctcg ttctgcggtg
     1201 gccgtccggc gccgatcgac caccagcgct tgagctcgaa ctgcgccgcc gggtcgacgc
     1261 ccatgagcaa ggcgcggcgg cccttgacct ccaccgcgcc gacgagctcc ggcgcgacga
     1321 cggcgatgct gccgcgcgcc gggatcgagc gcacacgctc gaggtcggcc tcgtccaggc
     1381 cctggtcgcc gacgctgacg ccgccgacgt cgatcccgcc gtacgtgagc gcgacgtcct
     1441 cggccttcgg cgtgatgacg atgttggcgc cgtagctctc gaggttggcc tcggcctcgc
     1501 cggccatgga ccgcgcgagg ctgagcagcg cgaccacggt cgcgacgccg atgagcaggc
     1561 ccgcgatgag aaaggccgcg cgggccttgc ggcggcgcag gttggcgaag gcgatggagc
     1621 gcagggtcat gcaggactcc ggatcagatg gcgcgcagcg ccactgtggg gtcgaggcgg
     1681 ctggcccgca tggccgggta cagcgagccc agcgatccga tgaggaccgc gagcagcacc
     1741 gccccggccg cgagcgccgg cgcccaggac acggtcacgt tgaggccctc ggcgaagaag
     1801 ggcagcagca ggtagctggc gcccatcccg accaggtagc cgagcacgcc ggccaccacg
     1861 ctggcgaccg ccgcctccat gagcacgagc agcgtcacgt gcgagcgccg gaagccgagc
     1921 gcgcggaaga tgccgatctc gcgagtccgc gcgttgaccg agcccatcat ggtcacgaag
     1981 accacgagcg cctcgatggc gatgatgatg gcggcgatca cgtagctgaa ggtccggaag
     2041 gtgtcgaggg cgtgcatgcg gttcctggtg acctgctgca tggccgtcac cttggtcccg
     2101 ggcagcacgt ccccgagctg caccgcgatc tcctccacgg ggcagtcgct gcagagcgcg
     2161 gccacctgca ccagcgtgac cttgcccggc ttgcccagca gcgcctgggc ggcgcccagc
     2221 tgggtgacga gcagctcgtc gtcctgggag cccgtcggac gcaggacgcc cgtgacccgg
     2281 aagtcacggc cgtccagggc gacggtgtcg ccgatgccga gatcgaggcg gcgggcggcc
     2341 gaggagccgg cgacgagctc gtcggcggcg gccggcggcc gcccgtccag cttccaccac
     2401 ttcttcagct cgaactcgtc ctcgggccgc acgccgagca gcagcgtctg gcggcccttg
     2461 gcctccaccg cgccgagcag ctccggcgcg acgatggcga cgttcttgct gttcgggatc
     2521 gtgctgatgc gcgagaggtc cgcttcgcgc aagtctcgcg cctgcagccg cacgccgccg
     2581 agcgccacac cgccgtagct cagcgagagg tcgtcggtgc gcggcgccac gacgatgttg
     2641 gcgccgtagg tctcgagctg gttctgcgcc tgcacggtga gcgcccggct cacggtgagc
     2701 agcgtcacca cggtggcgat gccgaccagc aggcccgcga cgaggaacgc ggctcgggac
     2761 gagcgccggc gcaggttgcc gagggcgacg gtgtgcagtc tcacgagacg ggatcaggcg
     2821 ttgcggacgg ctcgggtcag atctggtccg ggctcactgg aagtacttga cgccggtctg
     2881 caggtccgcg gtcttgagca ccaggttctg gccgtccacg ctgcgctcga tcggcgacgg
     2941 gttgcagccg cccctgacct cgttgatctg cgtggagtcg aactgctggc cgcagttgtt
     3001 gcagaccatc acgtcgccct gctgcgtgta gcccttcttg ttggcgaagc agacgtcaca
     3061 tgcgtcgaag gcggcacggt actcgccgtc cgcgctcttc atgacgaagt acttcacctc
     3121 gaccccgccg gcgtcgtacg cgtagtacct ggcctgaccg tcggcgagat cggccacggg
     3181 gatggtgacg tcggcgccgc cggcggccac gggagcggcg gcggccgggg cgccctgatc
     3241 gccgcggtcc atgaccacga cgagcgccac ggcgatgacc gccaccagcg ccacgccggc
     3301 cagggccaga tgcaggcccg tccggctctt cggctgctcg aactgcgcgc gtttgccggc
     3361 gcgcacgtct tccttgtgcg tgacgccccg ccggtccgtg gtagatgtgc gaccgctctt
     3421 cttccctgac aagtcgttcc tccccgtgcc tggccgaagc tgcgcccggc cgctctggtc
     3481 tctggtgcgc acgatggtag gcggccggga tgaagaagct gtgtggattc cgcgcctcct
     3541 ttcgctcgcc cccattcggg gtatgacgcg gctgtggcga cggagcacgg aggtgcatcc
     3601 tgggcgagtc cggcaagatc gtggtgatcg acgatgagga gtcggtgcgc gacgtcgtgc
     3661 gcgcctacct ggagaaggac ggctttcgcg tcttcgtcgc ggagaacggc cgcgacgggc
     3721 tggcgctcgc cgagcgccac cagccggacc tcatcgtcct cgacctcatg ctcccggacg
     3781 tctcgggcga ggagatctgc gccgagatac gggaccgctc cgacgtcccg atcgtgatgc
     3841 tcaccgccaa ggcgagcgag gaggagcgcg tcggcggcct cgtcgcgggc gccgacgact
     3901 acctcgtcaa gccgttcagc ccgcgcgagc tcgtcgcccg cgtcagggcg gtgctgcgcc
     3961 gcaccggcgg cgtcgagacg ccgctcgtgg aggtgctgcg cttcgacgac ggacggctcg
     4021 agatcgacac cgtcaagcgc gcggtccggc gcgacggcga cccggtggag ctcaccccca
     4081 acgagtacaa gctcctggtg gcgctcgccc gctatccggg ccgcgcctac tcgcgcttcg
     4141 agctcgtcag ccgcgtgcag gggtacgact acgagggtta cgagcgcacg atcgacgtgc
     4201 acgtgaagaa cctgcgcaag aagctcgagc ccgaccccgg caggccgcgg tacatcgaga
     4261 ccgtgacggg cgtcgggtac cggctcgcca aactgtgagc gggcgcatgc gcggcggcct
     4321 gaggacacgg ctggtgatcg ccttcgtggc cgtggccctg ctcggcgccc tggtcaccac
     4381 cgtgtactcg agcctcagcc tgacggcgcg cctcgagacc tcggcggagg cgcgcctcca
     4441 gaactcggcg ctgcacttcg gcgaggtggc cgcggtcgtg tcggacggca gcggctggag
     4501 ccggcagagc atcgagacgc tgcaccacct ggcgcagatc gaaacgctcg ccgcgcggct
     4561 ctacgacgtc cgcggcgagc tggtcttcga gcacccggcc tcggggcccg tcgagccggg
     4621 ggcggccgcg tcctcgccgg tcatggccgg agagcggcgt atcgggcgcg tggacatcag
     4681 ccgcgacgac gggcggctgt tcaccgaaga ggagatccgg ctccggagtc agttgctgcg
     4741 cacgaacctg atcgccggcg tgacctcggc ggcgatcgcc ctcgcggtcg cgctgtacct
     4801 cgcggtgacg ttgtcacggc cgctgcggca catccgcgcc ggggcggagg cgatgggcgc
     4861 cggcgacctc gatacccgcg tgcccgagag cggcgacgac gagatcgccg ccgtcgccgc
     4921 cgcgctgaac cgcctcgcgg agacgctgca acaggaggag gcgctgcgca aggagagcgt
     4981 cgccgacctg gctcacgagc tgcgcacccc ggtcatgggc gtcctcgcgc gcgtggaggc
     5041 ggcgcaggac ggcgtcctcg aggacgacgc cgcgaacctc tcggcgatgc acgacgaagc
     5101 gctgcggttg tcgcgcctgc tcgacgacct ctccgcgctc gccgaggccg agcgtccggg
     5161 gctgctgctg gacgcggagc ccgtggacct cgcggcgatc gcacgggcgc agaccacggc
     5221 gttcgccgcc gcgttcgcgg agaaggacat cgctctggag accgacctcc tgccggcgcc
     5281 ggtcagcggc gagcccaagc gcctcgagca ggtcgtcgtg aacctgctct ccaacgcgct
     5341 gcgctacacg gatgccgggg gtcgggtgct cgtcaccgtg cgtcccgacg gccaagccgc
     5401 cctcctcgag gtgtccgaca ccggcatcgg gatcgccgag gaggacctgc cgcgcgtctt
     5461 cacccgtttc tggcgcggcg agaagtcgcg ctcacgggac acgggcggcg cgggcatcgg
     5521 gctggcgatc gtcagggagc tcgtgcaggc gcacggcggt gaggtcggcg tggagagcgc
     5581 gccggcgcgg ggctccctgt tccgcgtcac gctgccgttg tccccgcatg cgtcccgctg
     5641 acgccgccgg tcttcacact ttctccacat cggggtgcga cactgcgtcc ggcgacggtg
     5701 aaccccttcc caaggatccc tggtcgcacc ggcgcgagga cgaccccccc tccaccgtcc
     5761 cgcgcctccc caagccccac ggcgcggggc cgctccccag gcggcccc
//
LOCUS       DASUMX010000101         6162 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1464503, whole genome shotgun sequence.
ACCESSION   DASUMX010000101 DASUMX010000000
VERSION     DASUMX010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 6162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6162
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1464503"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            496..2307
                     /locus_tag="VFZ86_04895"
     CDS             496..2307
                     /locus_tag="VFZ86_04895"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5641662.1"
                     /translation="MKTSLIEQLAAAPAVGQDYRPYALAPGGGAVAFEWYEGGDWQIY
                     LKDLPDGEPRRVGRLDDRCGCPQFSPDGRYLYFTCDDRGSECYDVYRYDLAAGKLVNL
                     LPDTPDLAPLPDPDLSPDGTQLALTVAHDAGYAVAVMPAEPDPGGSGLRWLTEHPYTE
                     SSPRWSPDGERLAVTTGTRGQDTAIVVIDVASGASRFVGGSDTFHAGHATWSPEGGRI
                     AFSGGPGDRPAIGIYELATGSVTWAWEDHHVDAHHPVWAPDGAALAFLVDLEGETGLL
                     HIDLRDGAVTEYSIGPGNHYAPEFTPDGAALVCVLSRPDAPPDLYRVELDDGDVTALT
                     DSLPGHLRDVPFVSGSPVWFTSWDHLAEVPGILVEPDEPNGAGVVIVHGGPTWHHANE
                     WDPLRQAFVAAGLTVLHPNYRGSDGYGRRWQLANRWLMGQGEVLDVAAAHEFLVQQGC
                     DPARIAITGRSWGGFHTAAALTQFPDLWAAGVAGVPFFDFIDSQLDPAIREDLRWWDR
                     ENTGDIERDRARLTYYSPINHLGRVQAPLLLLGGALDPRCPPRQIAEVAEALRARGQV
                     CDYVVYPDEGHEISGLAHRIDYDRRTVEFILEHTGSA"
     gene            complement(2374..3561)
                     /locus_tag="VFZ86_04900"
     CDS             complement(2374..3561)
                     /locus_tag="VFZ86_04900"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5641663.1"
                     /translation="MARVQMYSMEWCPYCAKAKALLGAKGIEYDEVDVTDDQEAALEM
                     VERTGQQGVPQFFIDGRWAGGYDNLAYLNATGVLDQMFGIQSTVDLEKVWDVAVVGGG
                     PAGLSAALYAARKNLSTILIALDLGGQVGITHLVTNYPGMPVVEGPELVKLMFEQAYM
                     YGLERMIGERVKNIRVDGRAKVLELISGKEVKARSVIVASGAQKRRLEIPGESEFAGR
                     GVVYCSTCDGPFYRDKIIAIVGGGSSALEAAIEMSGIAKKVHVVSRGPWSGEEWLKDK
                     ARSSPGVRPLVGYEPVEISGGEMVEKLVIRDAETGKQRALKVDGVFIEVGLSPNSDLI
                     LDLARTNQRGEVIVDEWGDTGVRGVFAAGDVTATKDKQIVIAAGEGAKAALAAFDYLI
                     SQR"
     gene            3736..4224
                     /locus_tag="VFZ86_04905"
     CDS             3736..4224
                     /locus_tag="VFZ86_04905"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX5641664.1"
                     /translation="MSRYDIENDIRETLGQIPEFFARMPDSTLEHEWAEFKRFQLEDS
                     ALTTREKELIGFGVAAAIHCPYCTYFHRSSAQMMGVSDEQIEEAARVAADTVKYSTYM
                     HALEIDFDAFKRMADEIGEYVSSREGQTGSAAPSGDETQGPPEGGSELDEIRSELRKD
                     AA"
     gene            4352..4600
                     /gene="purS"
                     /locus_tag="VFZ86_04910"
     CDS             4352..4600
                     /gene="purS"
                     /locus_tag="VFZ86_04910"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005543122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEX5641665.1"
                     /translation="MNVRVYVTPKQGILDPQGAAVERSLPNLGFEGVSGVRIGKIIDL
                     TIDGAGSEEAARAQVDDMCRKLLSNPIIEDYTFEITEG"
     gene            4608..5336
                     /gene="purQ"
                     /locus_tag="VFZ86_04915"
     CDS             4608..5336
                     /gene="purQ"
                     /locus_tag="VFZ86_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEX5641666.1"
                     /translation="MTAASPLRIGVVVFPGINTDGDTHHALETFPGVSAEYLWHKDTE
                     LKGVDGIVIPGGFSYGDYLRCGAIARFSPIMQSITDFAADGGLVMGICNGFQILIEAG
                     LLPGGLIRNTGLKFVCKYVNLRVETADTPFTGQCAQGDVLRIVVKHNEGNYTVDPATF
                     EKLQGNDQIVVRYVNAAGERAEGANPNGSLDDIAGVCNETRNVFGMMPHPENSVEAEL
                     AGGVDGRKFFGSMIDALRGAEVTA"
     gene            5333..>6162
                     /locus_tag="VFZ86_04920"
     CDS             5333..>6162
                     /locus_tag="VFZ86_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008204897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HEX5641667.1"
                     /translation="MSADRQPYVNRTADLAAAIKLGLTEDEYEHIKRIQDREPTYVEL
                     AIYSLMWSEHCSYKHSRPILGRFPTSGPRILQGPGENAGIIDVGDGWAVAMKVESHNH
                     PSAVEPFQGAATGVGGIVRDIFTMGARPCVSMDSLRFGELTEPRQRFLLDGVVGGIAH
                     YGNCLGIPTVGGEIYFEPSYEGNCLVNAFSAGLIRTDGIVRAIASGVGNHVVLIGSKT
                     GRDGIGGASVLASQEFDETLEEKRPSVQVGDPFTEKKLIEACLELIHRKLVVSMQDLG
                     A"
BASE COUNT          946 a         2273 c         2099 g          844 t
ORIGIN      
        1 cgccggcccc cctggggagg tgggggctgg gggatccagc gatcatcgcg gccgcccgac
       61 cggcgccgcg gagacaggcg ccggctcgag cgccggaggg ggacacggac ggaggcgcct
      121 ggcgtcgcga cgtccacgga gacgcgcggc cgttccgtgc ggccgaaccg cggcagtacc
      181 catcgccatc acctcccgga ccggccgagc cgcctgacag gtcggccctg atcgccgccg
      241 gcgctggcgc gcggtgggca ggtgatcgtg gcacccgcag tgtagcggga ggcgccgcgc
      301 gcgtcccggt ggtccggacg gcgccggggt cacataccgt ggtgacgttg cccccgcaga
      361 gtgccccgca gagtgccgcg tcacgtgcgg ggcgccccgc ggcgcgctag actgagcccc
      421 gcgagggtcg cgcgcccgca gcgggacgcg gccccttctc caggccttgg ggcagcagcg
      481 accggggggt catccgtgaa gacgtcgctc atcgagcagc tggccgcggc gccggcggtg
      541 ggacaggact acaggccgta cgcgctggcg ccaggcggcg gcgccgtcgc cttcgagtgg
      601 tacgagggcg gcgactggca gatctacctc aaggacctgc cggacggcga gccgcgacgc
      661 gtcggccggc tggacgaccg ctgcggctgc ccgcagttct cccccgacgg ccggtacctc
      721 tacttcacct gcgacgaccg cggctcggag tgctacgacg tctaccgcta cgacctcgcc
      781 gccgggaagc tcgtcaacct gctgccggac accccggacc tcgcgccgct gccggacccc
      841 gacctctccc cggacggcac ccagctggcg ctgacggtgg cgcacgacgc cggctacgcg
      901 gtcgccgtga tgccggcgga gccggacccc ggcggcagcg gcctccgatg gctcacggag
      961 cacccctaca cggagagctc gccgcgctgg tccccggacg gcgagcggct cgccgtgacc
     1021 accgggacgc gcggccagga cacggcgatc gtggtcatcg acgtggcctc gggcgcgtcc
     1081 cgcttcgtcg gcggctcgga cacgttccac gccggtcacg cgacgtggtc gccggaaggc
     1141 ggccgcatcg ccttctccgg cgggccgggc gaccgcccgg cgatcggcat ctacgagctc
     1201 gcgaccggct cggtcacgtg ggcctgggag gaccaccacg tcgacgccca ccaccctgtg
     1261 tgggcgccgg acggggcggc gctcgccttt ctcgtggacc tcgagggcga gaccgggctg
     1321 ctgcacatcg acctgcgcga cggcgccgtc accgagtaca gcatcggccc cggcaaccac
     1381 tacgcgcccg agttcacgcc cgacggggcg gcgctggtgt gcgtgctcag ccgccccgac
     1441 gccccgcccg acctgtaccg cgtggagctg gacgacggcg acgtgaccgc gctcaccgac
     1501 tcgctgccgg ggcatctgcg ggacgtgccg ttcgtctccg gcagcccggt ctggttcacg
     1561 agctgggacc acctcgccga ggtgcccggg atcctggtcg agccggacga gcccaacggg
     1621 gccggcgtcg tcatcgtcca cggcggcccg acctggcatc acgccaacga gtgggacccg
     1681 ctgcggcagg cgttcgtggc cgcgggcctc acggtgctgc accccaacta ccgcggcagc
     1741 gacggctacg gccgccgctg gcagctcgcc aaccgctggc tcatgggcca gggcgaggtg
     1801 ctcgacgtgg cggccgcgca cgagttcctc gtgcagcagg ggtgcgaccc ggcgcgcatc
     1861 gcgatcacag ggcgcagctg gggcggcttc cacacggcgg ccgccctgac gcagttcccc
     1921 gacctctggg cggccggcgt cgccggcgtg cccttcttcg acttcatcga cagccagctg
     1981 gacccggcca tccgtgagga cctgcgctgg tgggaccgtg agaacaccgg ggacatcgaa
     2041 cgggacaggg cgaggctcac gtactactcg cccatcaacc acctcggccg cgtgcaggcg
     2101 ccgctcctgc tcctcggcgg cgccctcgac ccgcgctgcc ccccgcggca gatcgcggag
     2161 gtcgcggagg cgctgcgcgc gcgcggacag gtctgcgact acgtcgtcta cccggacgag
     2221 ggccacgaga tcagcggcct cgcgcatcgc atcgactacg accggcgcac ggtggagttc
     2281 atcctcgagc acaccggcag cgcctgacgg acgggcgccc ccgccggcgg cccggctccg
     2341 gggcggcgcc cggcgcctgg ccgggcgccc cgctcagcgc tgcgagatca ggtagtcgaa
     2401 cgcggccagc gccgccttgg cgccctcacc cgcggcgatc acgatctgct tgtccttggt
     2461 ggcggtgacg tcgccggcgg cgaacacgcc gcgcacgccc gtgtcgcccc actcgtcgac
     2521 gatcacctcg ccgcgctggt tggtgcgcgc gaggtcgagg atgaggtcgg agttgggcga
     2581 gagcccgacc tcgatgaaca cgccgtccac cttgagcgcg cgctgcttgc cggtctccgc
     2641 gtcgcggatc acgagcttct cgaccatctc gccaccggag atctccaccg gctcgtaccc
     2701 gacgagcggc ctgacgcccg gcgacgagcg cgccttgtcc ttgagccact cctcgccgct
     2761 ccacgggccg cgactgacca catgtacctt cttggcgatg ccgctcatct cgatggccgc
     2821 ctcgagcgcc gagctgccgc cgccgacgat ggcgatgatc ttgtcgcggt agaacgggcc
     2881 gtcgcaggtg gagcagtaca ccacaccgcg gccggcgaac tcgctctcgc cggggatctc
     2941 gagccggcgc ttctgcgcac cgctggcgac gatgaccgag cgcgccttga cctccttgcc
     3001 ggagatgagc tcgaggacct tggcgcggcc gtccacgcgg atgttcttga cgcgctcgcc
     3061 gatcatccgc tcgagcccgt acatgtaggc ctgctcgaac atgagcttca cgagctccgg
     3121 gccctcgacc acgggcatgc cggggtagtt ggtcacgagg tgggtgatgc cgacctgacc
     3181 gcccaggtcc agcgcgatga ggatcgtgga gaggttcttg cgcgcggcgt acagcgcggc
     3241 cgacaggccg gccggtccgc cgccgaccac cgcgacgtcc cacaccttct cgaggtccac
     3301 ggtcgactgg atgccgaaca tctgatccag gacgccggtc gcgttgagat aggcgaggtt
     3361 gtcgtacccg ccggcccagc gaccgtcgat gaagaactgg gggacgccct gctgaccggt
     3421 gcgctccacc atctccagag cggcctcctg gtcgtccgtg acgtccacct cgtcgtactc
     3481 gatgcccttg gcgcccagca gggccttcgc cttggcgcag tacgggcacc actccatcga
     3541 gtacatctgt acgcgagcca tttgctacct ctcaggtcag atactcgtta ctcagcgtgg
     3601 tgattcccat gcgcaaacgt gttgacgccg cgccggcccc aacgcgggac ggttttgccg
     3661 gccccggccc gggaaccgca cctgtatgcg ccgaaggcgc agttgtccgc caccagaccg
     3721 gcacggaggt ccgtgatgag ccggtacgac atcgagaacg acatccgcga gacactggga
     3781 cagatccccg agttcttcgc gcgtatgccc gactccacgc tcgagcacga gtgggccgag
     3841 ttcaagcggt tccagctcga ggacagcgcg ctgaccacgc gcgagaagga actcatcggc
     3901 ttcggcgtcg ccgcggccat ccactgcccc tactgcacgt acttccaccg cagctcggcg
     3961 cagatgatgg gcgtcagcga cgagcagatc gaagaggcgg cgcgcgtggc cgcagacacg
     4021 gtcaagtaca gcacctacat gcacgcgctc gagatcgact tcgacgcgtt caagcgcatg
     4081 gccgacgaga tcggcgagta cgtgtcctcc agagaggggc agaccgggag cgccgcgccg
     4141 agcggcgacg agacccaggg cccgcccgag ggcggctccg agctcgacga gatccgctcc
     4201 gagctccgca aggacgccgc ctgaggccgt gaggacgccg cgggaggccg gccggagggg
     4261 cggcgcggcg cgcggcgccc cgcccctccg gccgccatct gcgatacact cgggcctgtc
     4321 cccagtacca actctccggg caggtgagtc gatgaacgtg cgcgtctacg tgacccccaa
     4381 acaaggcatc ctcgaccctc agggcgcagc cgtggaacgg tcgctcccca acctcgggtt
     4441 cgagggggtg agcggggtgc gcatcggcaa gatcatcgac ctcaccatcg acggcgcggg
     4501 ctccgaagaa gccgcccgcg cccaggtcga cgacatgtgc cgcaagctcc tctccaaccc
     4561 gatcatcgag gactacacct tcgagatcac cgaggggtga cgccgcgatg accgctgctt
     4621 ctcccctgcg catcggcgtg gtggtcttcc ccgggatcaa caccgacggt gacacgcacc
     4681 acgccctcga gaccttcccc ggcgtcagcg ccgagtacct ctggcacaag gacaccgagc
     4741 tcaagggtgt cgacggcatc gtcatccccg gcgggttcag ctacggcgac tacctgcgct
     4801 gcggcgccat cgcccggttc agccccatca tgcagtccat caccgacttc gccgccgacg
     4861 gcggcctggt gatgggcatc tgcaacgggt tccagatcct catcgaggcc ggcctcctgc
     4921 ccggcggcct catccggaac acgggcctca agttcgtctg caagtacgtg aacctgcgcg
     4981 tcgagaccgc ggacacgccg ttcaccggtc agtgcgcgca gggcgacgtt ctgcgcatcg
     5041 tcgtcaagca caacgagggc aactacaccg tcgaccctgc gaccttcgag aagctgcagg
     5101 gcaacgacca gatcgtcgtg cgctacgtga acgccgccgg cgagcgggcc gagggagcca
     5161 accccaacgg ctccctcgac gacatcgccg gcgtctgcaa cgagacgcgc aacgtcttcg
     5221 gcatgatgcc gcacccggag aactcggtgg aggcggagct cgccggcggg gtcgacggac
     5281 gcaagttctt cggctccatg atcgacgccc tgcgcggcgc ggaggtgacg gcatgagcgc
     5341 cgacaggcag ccctacgtga accgcacggc cgacctcgcc gcggcgatca agctcggcct
     5401 gaccgaggac gagtacgagc acatcaagcg gatccaggat cgcgagccga cgtatgtgga
     5461 gctcgccatc tactcgctca tgtggagcga gcactgcagc tacaagcact cgcggcccat
     5521 cctggggcgc ttccccacca gcggcccgcg catcctccag ggccccggcg agaacgccgg
     5581 catcatcgac gtcggcgacg gctgggccgt ggcgatgaag gtcgagagcc acaaccaccc
     5641 gtcggccgtg gagcccttcc agggcgcggc caccggcgtc ggcggcatcg tgcgcgacat
     5701 cttcacgatg ggcgcgcgcc cgtgcgtgag catggactcg ctgcgcttcg gcgagctcac
     5761 cgagccgcgc cagcgcttcc tgctcgacgg cgtcgtgggc ggcatcgccc actacggcaa
     5821 ctgcctcggc atccccaccg tgggcggcga gatctacttc gagcccagct acgagggcaa
     5881 ctgcctggtc aacgcgttca gcgccggcct catccgcacc gacggcatcg tgcgcgccat
     5941 cgccagcgga gtcggcaacc atgtggtgct catcggctcc aagaccggcc gcgacggcat
     6001 cggcggcgcc agcgtgctcg ccagccagga gtttgacgag acgctcgagg agaagcgccc
     6061 gagcgtccag gtcggcgacc ccttcaccga gaagaagctc atcgaggcgt gcctcgagct
     6121 catccaccgc aagctcgtgg tgagcatgca ggacctcggc gc
//
LOCUS       DASUMX010000102        24984 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_2041438, whole genome shotgun sequence.
ACCESSION   DASUMX010000102 DASUMX010000000
VERSION     DASUMX010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 24984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24984
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2041438"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="VFZ86_04925"
     CDS             complement(<1..611)
                     /locus_tag="VFZ86_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641668.1"
                     /translation="MADQTSPAPASLQVYRYRAGADRVEAVIGALEPDLPHEDLVAHA
                     QEALAHETFMCYAVSQSDDFVADELCFVPQGDRCDILSIRTNGWQRHHGDHRRSLVEI
                     LNNLAAAVACGKLRILDAQELAGEVGRGRPAGAFEIHDTAELQKARETVDEVLTREGV
                     DDINRQRTILCISEAVTNMLLHGGGHGSMRVRRLDDRLRFVVA"
     gene            complement(649..2187)
                     /locus_tag="VFZ86_04930"
     CDS             complement(649..2187)
                     /locus_tag="VFZ86_04930"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HEX5641669.1"
                     /translation="MSAAETPAAIGHDEAAVRRRTAVSLYVALVCAAAAGVSVAAALD
                     EPAPFTLNLVAFMILATLTDLREIKLPWVGDVTLSFVPVLACLIALGLWPAMAVAAVS
                     GITAAWVTRDPVKIAFNVGNYVVSTYLAGLLYLALTPEGGAFMTKVLPTFGATIVDFL
                     AHTALLAGVIALSTQDGGAVRVWRRNYQWALPGYLAGSTLALLVAALYLWLGVPGMLL
                     GIPPLYLIYYSYDIYVKRAREKVTFDAERDSFHKELAASVELHDELRAAQLKVAAEIE
                     RARRIQTDLLPATIPEVPGIALEQRIEFLGEMGGDYYDYIPFGDGRLGIVCGDVMGKG
                     LAAALIMAMARSLLHDAIAPGKRAGDVLAEVNDGLTRDLEGQRLPYFLTLALAVYDPA
                     DRSLVVAGGGHNPVLVAGRDGLREVPSRGAALGVRTGLRFPEDTLVLEAGATVALYTD
                     GLTEARDPEGSLFGLERLERSLERCQDAPVRDTLEGVWGDIVAFRGPASRGDDATLLL
                     VRLL"
     gene            complement(2184..3902)
                     /locus_tag="VFZ86_04935"
     CDS             complement(2184..3902)
                     /locus_tag="VFZ86_04935"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HEX5641670.1"
                     /translation="MKMRAKYLVLAMALLCLLAVPSPAGAASDEAVKIDDSVTTVLAA
                     TGGSDAVPVIVYTDPGAQGVVGRTVPSGVETTTLAGFDAVAAYLTEAEIDALAASDAV
                     ALIVADNPVFGFDYASSMDVTNLAIGLDRVAAPAAGGPSGAGVGVAILDSGIATTSDL
                     QSSRIVGWKDLVHNRKAPYDDAGHGTFVAGLIAGDGTASLPLDQHGYATMQFRGVAPA
                     ADIIGVKVLDKTGQGRASSVMSGILWVIAHKDEYNIRVLNLSLGSNPVAPADFDPIAR
                     AVELAWKNGITVVCAAGNEGEFGPGGILSPGNSPYVITVGATDTRQTASLDDDAVTFY
                     SSVGPTLWDEYAKPDVVAPGNRLISLRVKDSYIDSEFPQNLIPVASYVPGAPADAEPS
                     YLMLSGSSTSAPVGAGVAALMIGEDPDLSPDDVKVRMMGTAKALPDTDRYEQGAGLID
                     AAGALESDAQADGYALSEDVGDGTTVLTDADYAAWDKVAWAKYGWTKFRWTKFRWTGV
                     SWNKFRWTGVSWNKFRWTKFRWTNVAWTKFRWTKFRWTDYQATKFRWTEYEWAKFRWT
                     VLIEGQ"
     regulatory      complement(3988..4068)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            4379..5800
                     /locus_tag="VFZ86_04940"
     CDS             4379..5800
                     /locus_tag="VFZ86_04940"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX5641671.1"
                     /translation="MSRTGGLYTAAQRQVINQMLRLAASDDKRKIVKAFRLAEKITPD
                     KHKSSVRFVTEKVQDDHPALLIAKHVTSKLSPRCRDAFIECLIVNTLLRGVDKRMSLV
                     ESTGNNAPTTILMSPTMRCNLTCEGCYAAEYSPDQDLDRGLMQKIVDEGNDMGVYLFT
                     LLGGEPFMWDELLDFAAENKDAYFQVFTNGTLLDHDTIDELARVGNIAPMLSLEGSPE
                     LTDHRRGDGVHEQVMEIMDELGEAGVLFGYSCTVARNNWRALVSDAFVDPLIAKGALL
                     AWHFLYMPVGRDPDTSLMLTAEERNQFRVEINRIRDTKALFPVDFWGDAPWVKGCIAG
                     KHYMHINSGGWVEPCIFTHFATDNIRDVSLLEAFNSDFFKEIRRRQPFNHNLLMPCMW
                     IDNPTQAREIMAATGAKPTHPGGDVMITDLQDELDSYARSVDRLYSPIWSCLSAEKED
                     AADKHAKTAAMVGGTRRDKMPVG"
     gene            5953..8475
                     /locus_tag="VFZ86_04945"
     CDS             5953..8475
                     /locus_tag="VFZ86_04945"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HEX5641672.1"
                     /translation="MARPRPHKRAARLAAIFTLSVLGAVAAVWAAPRVSGAVEAWMST
                     PPVELRTTNLGAALPVVPETGATGVTGGRAAAAAGDGSVATDPAASGDTLAPAAPAAA
                     ATVDAGMRFTMAGVRCAPPARAGEVRVRLRTSEDGRSWSAWYAVTLERVAEEGGEELA
                     FTEPIWTGGGRYLQVVAEAGGGDEPVPVRLRDVRVVTINSTEDADRTAVAVGVLRRAA
                     AAVAGLRLTPDAAAMTTKPKIVTRAAWGANESWRSGSPDYAAVKMAFVHHTASSNGYT
                     AAEAPAVVRGVYAYHTRSLHWSDIGYNFLIDRYGTIYEGRYGGVARGVVGAQVLGFNT
                     GSTGISVLGTFSDSTPPSRTVTALERLLEWKLDVHHIDPLGTGTLRCAYGQKYATGQR
                     VTFPAIAGHRDANYTDCPGGRLYAQLPTVRRVVARTGQPKIYGFIVADPSISPNGDGV
                     RDRTTVGFTVSQSASWTVTIRDDAGQPVRNLSGEGTEVETTWAGHDDTGAALPDGVYT
                     LQADATSAAGVARPATATLRLDTVTPRIESAVIEPDPFSPNGDGQADVTRLAFVPGES
                     GTARVSVLGAGDAVLRRVTGWTSVMAAVRRVTWDGRVSSGSALEPAPEGEATLLLELR
                     DGAGNTTSARRKVTLDRTLARRSVSRTTFSPNGDGVHDGVTLSFRLTRAAEVTVTVVR
                     QGSTMRTMRLGRLGAGNHSVTWDGKLGGGGTAASGAYSLKLTATGALGSTSVVQPLTV
                     DLSPPRLTAPATASVRYGRTAKIAYTVRDAFSPKVKVSATVTDARGRTVATLALGWVK
                     QGVAQVCAWKPRARRTYTVTFKALDLGGNRQAKPAVTSLRVR"
     gene            complement(8533..8691)
                     /locus_tag="VFZ86_04950"
     CDS             complement(8533..8691)
                     /locus_tag="VFZ86_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641673.1"
                     /translation="MVWEILAWAAVVVLYAFRIALVVGILAVVVMVVRTLRVPRAPAR
                     HAGAPARR"
     gene            complement(8869..9660)
                     /locus_tag="VFZ86_04955"
     CDS             complement(8869..9660)
                     /locus_tag="VFZ86_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641674.1"
                     /translation="MNESTTLTNRERRENVAFLGQAFLAAPKLPSEILDAEQRDRFSS
                     DEAEQPRLFFTLLCYGALAAQPAPDSVVDADWRRFADAAVVDALFAGEFDLKRYALDP
                     PVSRNDSKGKIIDFVRLAETRDTWGRLLPQPPDPDVAVDVLHAASGGALKSRAFWAVR
                     EMRRYGLWDDEALDRFACVPDGRVRKRAVRMGLVDLPEKADTFADMKAVSRALHAVLR
                     LGESPEGCFDLPVSMADQRCEVCDAARMAACPMPHCRWRREHAEA"
     gene            9957..13067
                     /locus_tag="VFZ86_04960"
     CDS             9957..13067
                     /locus_tag="VFZ86_04960"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HEX5641675.1"
                     /translation="MTTIGIDIGCISLKAAFVGQAGDQDLFRELLSQHPDLFQTPRDG
                     LTVVGEAPVLVARYRRIKGGPAEAVDALLSDVLAVLPEGSVRGARVTGSGGRLVGAAL
                     DASFENEFKAITRGIAALHPETQTIFEMGGETSKFIRLEASEIGCAGITDYQTNGDCA
                     AGTGSFMDQQASRLLYDIEDVGDIVLDAGKAASIAGRCSVFAKSDMIHAQQKGYQPPE
                     VLRGLCDAVMRNFKGTIAKGKVVEPPVAFIGGVAANDGAVRSLREAFALDDGQLFIPE
                     YHASMGAVGAALIEADLEAARDRALAGLDTERLPAAAFPVTEPLSMERVVLLRDMAKP
                     YVFSDAREVVDAYMGIDIGSVSTNLVVIDDEGEVIKEIYTKTDARPVEVVSKGLADIQ
                     SEIGARINLLGVGTTGSGRELVGELTGADIITDEITAHKTGADFIGRRIGKQCDTIFE
                     IGGQDSKFISLQDGIVVDFAMNEACAAGTGSFLEEQAEKLGINIIGEFAELALGSPAP
                     IRLGERCTVFMERDVMAYQQRGARRDDLVGGLAFSIATNYLNRLVRERHVGDVIFFQG
                     GTAYNDSVAAAFSQILDKEIIVPPYNGVMGALGMALLARERVKRTGEPTTFRGWDLEQ
                     VDYTVVDFVCKACSNECDVRQFTIEGEKTYWGDKCSDRYRKPAKVDKEPVIADLVALR
                     EEALMAPYEAARARLPETAPTVGFARAMYTYDRLPFWATFFAELGLRPVLSPESDKQI
                     REAGVALTVAEPCFPIRVAHGHVQWLLNEGVDWVFVPNQVNEETEFEQYNSHACPWGQ
                     TLPFVIKTAPGLQPHKHQILDPRVRFRDGREGLARDFAGVAQKLGVSASKLSGAFAAA
                     YQAQDAFRDTLLAAGAEALERLELAGELGVVLIGRPYNMYDKGINMDIPRKLRKFYGV
                     NVIPLDFLPIRGIDIDPIVPNMYWNYGRKILQTATFVRDKERLHLIYMTNFKCGPDSY
                     IKHYVREASGKPFLTLQFDEHQNDAGAMTRCEAYLDSKGFLRWWSQDAADEAVVCREE
                     VAGVVE"
     gene            13054..14397
                     /locus_tag="VFZ86_04965"
     CDS             13054..14397
                     /locus_tag="VFZ86_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641676.1"
                     /translation="MSSNKPAGGAAAREGSPQDPRIQQAIADFGLMGRRLYLPQMPYG
                     GSYLLAAAIRSIGFDAWVTSDSDERTLDLGGRVTSGDECYPQKITLGDFLRIIEDEGR
                     DNVAFLMPSANGPCRFGQYLHLVRAKFDELGYDDVPVISITSSDGYSSIGAYSQNLIR
                     TAWRAVLAQDILMKLLLKTRPYEREKGASDAAYQTSLEEAGEAIARVGISHKEQLAGL
                     IPVMERARDRFRAIPADYDATRPLIGVVGEIFCRQNTFSNFDLIRVVEEQGGECWLSD
                     IGEWVWYTDDEHRRRLRDERRRVSKDGAIRFIKNKIMRRDEHAMYGPFHDDFVGYEEP
                     QDVRDVLEMSFPYLPYTGALGEMVLSSGKAIYLYEKGADGIIDISPFTCMNGIVTEAV
                     YPSLSKDLDDLPIRVFYFDGTQGDLERDVGIFIELARTYQRRKHKTRRYPAYFSV"
     gene            complement(14541..14693)
                     /locus_tag="VFZ86_04970"
     CDS             complement(14541..14693)
                     /locus_tag="VFZ86_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641677.1"
                     /translation="MQNRIFRSIGRFFEGTGSAVSTPSGDARGHLKVPIDTMFDVSAG
                     TRWMGR"
     gene            complement(14713..14862)
                     /locus_tag="VFZ86_04975"
     CDS             complement(14713..14862)
                     /locus_tag="VFZ86_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641678.1"
                     /translation="MAERLAGIKRIIEVAVTSVATSPQGVRGPLRGRDFMLDDMTFGA
                     DRDGL"
     gene            complement(15027..15863)
                     /locus_tag="VFZ86_04980"
     CDS             complement(15027..15863)
                     /locus_tag="VFZ86_04980"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein HesB"
                     /protein_id="HEX5641679.1"
                     /translation="MSGETVGLPSVRVVRALQTRLLAWYAENRRDLPWRRTADPYAIL
                     VSEVMLQQTQVPRVSPRFVEWLEAWPDLESLAAAPLADVLRRWQGLGYNTRARRLQEC
                     AAAAVASAPPERRAELPRSLDGLRRLPGVGPYTARAVLVFAHNDDLAAVDANVRRVLS
                     HELGLPEDLGDRDLQAVADTVLPRGRSRDWHNALMDYGSLVLTARSTGIASRSRQGAF
                     EGSRRQKRARALRRLLAHGPQPLEELAAALELAPGEAAEVVDGLRRDGLVTLSGGTVD
                     VA"
     gene            complement(15932..17884)
                     /locus_tag="VFZ86_04985"
     CDS             complement(15932..17884)
                     /locus_tag="VFZ86_04985"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEX5641680.1"
                     /translation="MSIVTFNNVEKSYAGQRLFTTVSFSIDQHDHAVLVGRNGTGKTT
                     LLRLLSGDEHADSGTIARARGRRIWLHEQTPELERDRPVHEYLLEAFGEAVELEAALR
                     RTEERLSGLAEHSPELRDTMRTYQQLQRRFEAAGGYGYRDRLGGVAEGLGLPDAMLER
                     NLLSLSGGELTRVTLARALLADADLLLLDEPTNHLDIDSVEWLEGFLSGYERAFLLVT
                     HDRRLLEKVGRRVLAVEHEHVETQGGDFATYLRESEARLDLMRREYEQDLEKIEQLQR
                     FYDRFHAKKDKAKQAKAKLTQIERIKAGLREPPRQKRSFKLGLPQPKPSARVVLELKA
                     PAIGVGSADDAAGAHTLVHDVDVVLERGEKVALLGPNGSGKTTLIETVAGLRSLPAGA
                     SHLGHNVRAAYYSQQGRELREEDTVLQSVLPLVGQDEQSARGLLGRFQFGPDEVEKRV
                     EMLSGGERSRLRLLTLLVGDANFLLLDEPTNHLDMGSVEALADALEDYTGTILLVTHD
                     RHLIDRVATRVLEIQDGRLLNHLSAARYWEARNERLGIAPAATPEPPRGGRRAATAAP
                     AGAAVTRGKESAEEARRRRARLRGAEAGILKAERRLGEIDAELAEPDTYADRDVLNRL
                     LDERSAVEKRLQREYAAWEKLVDEEA"
     gene            18022..19041
                     /locus_tag="VFZ86_04990"
     CDS             18022..19041
                     /locus_tag="VFZ86_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641681.1"
                     /translation="MSVGRAQDILIGPRTTVHALLLAYPFLESYLLARVRGFERLRDR
                     RARTRWARVMTLDDVGVRLNVPWRQLVREIAAEVEQRTGRPARVADAPQRVVDDGRRI
                     GELRGIVEGLEAGAPLLEMAARWGAATGDLERAEVDALDAALSEAALAGRAGGERVAL
                     AAAPGDIALAEPPPGHPLETLRREARHVLLLTAGLRAEMDRLGGSPPRRRWQRAKPLV
                     ARLADRLSAVELRFRREQQAWFPALEVLGIEGPQALLAPRQGEALETLRRLRLAVDRD
                     DAPAAVEAGTRLVDALEDLLAQDERLLEPLALRHLSPEDWAAVRELEDGVGWGLVVPP
                     PWPDS"
     gene            19090..19902
                     /locus_tag="VFZ86_04995"
     CDS             19090..19902
                     /locus_tag="VFZ86_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641682.1"
                     /translation="MDGRYPGDGLLETQDLMAQEGRRVVDTARRRGVVLRLLGGFAVH
                     EHCGGKTACRRDHLDLDFAALSRQTRPVVEVFEELGYEERIHVRQATRMMQAQFVREC
                     VHGGGGVPRAHGEDHVDVFFDRFKLDHSLDLRDRLAQHPYAVPLTDALVTKLQMHAPE
                     PRDVRDAMFLLAAGAEGGEAGDVDPTYVGRLCARDWGLFYDVALSLQRCVRALDEAGL
                     GPEERDRVAGLLARLTGALDAAPKTLAWRLRAGVGTRRRWWNVVEEQGRLTA"
     gene            complement(19892..20830)
                     /locus_tag="VFZ86_05000"
     CDS             complement(19892..20830)
                     /locus_tag="VFZ86_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641683.1"
                     /translation="MDTPPTSRLHDAALDATLRRHERLMAEAHAIIDAGTRQDVVLRL
                     TGGLAVRHYAIDLEFAEREYSDIDLIGLKRQVVAVGQVFRGLGYIENRHAAMATGNGQ
                     LQFFLPGAACASAPGAGGDAAPDDLPVYAEVPPSDHVDVFLDAMRMDHQIDFRDRLTI
                     NTYAIDPADLFLSKLQIVHLNEKDCHDIVTLVKDVYVDFQPHPGVLDLHHVAGVCAAD
                     WGLYIDVMNNIDTVIDHVADYDLSPRDAARVRRTLELAQDMMTEQAKTLRWRLRARIG
                     KRVRWYDEVEEQFGGRTEDMSLAVRTKKGRAASLRP"
     gene            complement(20844..21809)
                     /locus_tag="VFZ86_05005"
     CDS             complement(20844..21809)
                     /locus_tag="VFZ86_05005"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HEX5641684.1"
                     /translation="MRDCTRREFLKIAGLAAAGASLAAAGCGSQPAADPPQEGRLSSP
                     PASPGGKRGQSVLSVARGGDAAEITAAAVAALGGMEAFVRPGADVVVKPNICTGYHGP
                     EYAATTNPEVVATLVELCLRAKARRVRVMDLPFGSPAEEAYRVSGIQDAVERAGGEMQ
                     IMSPAGFREFRIPDGRDLSSWPIYTDVLSCDALIDVPIAKDHGSTRLSLGGKNLLGVI
                     LDAGDIHTNIGQRTADLVSVCRPTLTVVDAVRVLTANGPTGGDLDDVERLDTVLASAD
                     IVAADAYAATLFGLTGADIPYVQAAHDMGLGEMDLEKVEVRAVKA"
     gene            21910..23124
                     /locus_tag="VFZ86_05010"
     CDS             21910..23124
                     /locus_tag="VFZ86_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641685.1"
                     /translation="MNIGDVFARAWDLWRRDVGWLILAGLVVGLIMAVIFGIAFALFA
                     AVFAGAGVTIGADLANDTTSSLTGLGASLLVVGFIVYIVAMFLIQVVAMTFYGGMFEM
                     VIGAYRGDRGVEFGDLFSGFRKIGAYALYALVLFGISLGLSLLNILPLIGGVISFVVS
                     LWITVIWLYVLPLIADQGLGFGEAASRSNQMVKGAGWWWTFGMVVLLGLALIAAAVVI
                     LLVAWGAYQGSEWVGIAVGILLFLVFAVLFPPYAICYVSVLYIASGGDIAVAATPAGG
                     LPGIPPAPPAPPTYGTPASPSAAGPPGATGVTAPVVPPATGADAWRAAADPLAGQPPA
                     PPPAPAPSAAPAVTAADEAAVPAGESAAAAGQAAVEPQTAITQVDAESSEPPAPPAPP
                     APPAPSPPGGGV"
     gene            23482..23901
                     /locus_tag="VFZ86_05015"
     CDS             23482..23901
                     /locus_tag="VFZ86_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008560065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HEX5641686.1"
                     /translation="MVLKDIVDTAVEAGDFTTLVSAVQAAGLEETLRGEGPYTVFAPT
                     DDAFAAVPAETLDALLADPTGALTDVLTYHVVPGKVMSTDLSDGMQATTVNGQALDIT
                     IAEDGTVMVNGATVITADIETANGVIHVIDTVLVPSE"
     gene            complement(23971..>24984)
                     /locus_tag="VFZ86_05020"
     CDS             complement(23971..>24984)
                     /locus_tag="VFZ86_05020"
                     /inference="COORDINATES: protein motif:HMM:NF012907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HEX5641687.1"
                     /translation="AALALATALDDDRPHGPYLIGAWTFGDRGSLETAVDAGALDEVS
                     ADWLQSRADGSVVAPRLDTGFIEAARDADCRVFVTVTDYDEAEHAFDPSISATILRTA
                     ATRRRHAEAVADWCREYDVAGVDVDWEAVKGSRRDAFSAFVEELAARLHADDRLIAVD
                     VYPKLSEPGGWDGPRGQDWARLGRAVDQFRVMSYNYSGSWSGPGPLSPPDWMDRVLSF
                     AETQVAPRRIVMGLGFYGRDWGGSGTTDLVWDDVRAIRAELDPRETRTASRELRLAYR
                     RDGASHEAFFPDAPAVRAKARMLLSRHPRIRGVYAWLMGQEDPRVWQELARLLHTGAR
                     GQR"
BASE COUNT         3597 a         8847 c         8948 g         3592 t
ORIGIN      
        1 tcggcgacga cgaagcgcag gcggtcgtcc agccggcgga cgcgcatgct gccgtggccg
       61 ccgccgtgca gcagcatgtt ggtcacggcc tccgagatgc agaggatggt ccgctggcgg
      121 ttgatgtcgt cgaccccttc ccgcgtcagg acctcgtcca cggtctcgcg agccttctgc
      181 agctcggccg tgtcgtggat ctcgaacgcg ccggccggtc gcccccggcc gacctcgccg
      241 gcgagttcct gcgcgtcgag gatgcggagc ttgccgcagg cgacggccgc ggcgaggttg
      301 ttgaggatct ccacgaggct gcggcggtgg tcgccgtggt gccgctgcca gccgttggtg
      361 cggatgctga ggatgtcgca gcggtcgccc tggggcacga agcacagctc gtcggcgacg
      421 aagtcgtccg actgggacac cgcgtagcac atgaaggtct cgtgcgcgag cgcttcctgc
      481 gcgtgggcga cgaggtcctc gtgggggagg tcgggctcga gcgcgccgat gacggcctcg
      541 acgcggtccg cgccggctcg gtaccggtac acctgcagcg aagccggtgc agggctcgtc
      601 tggtcggcca tcctcgctgg ctcccgtcgg tccgtcgtcc tctccgcctc agaggaggcg
      661 gacgagcagg agggtggcgt cgtcgccacg ggaggcgggc ccccggaacg cgacgatgtc
      721 gccccacaca ccctcgagtg tgtcacgaac gggtgcgtcc tgacagcgtt cgagcgagcg
      781 ctccaggcgt tccaggccga acagcgaccc ctcggggtcg cgcgcctcgg tcaggccgtc
      841 ggtgtacagg gccacggtcg cgccggcctc caggaccagg gtgtcctcgg ggaaccgcag
      901 cccggtgcgc acgccgaggg cggcgccccg cgagggcacc tcccgcaggc cgtcccggcc
      961 ggcgacgagc accgggttgt ggccgccgcc ggcgacgacc aggctgcggt ctgcagggtc
     1021 gtacaccgcg agggccaggg tgaggaagta cggcaggcgc tgcccctcga ggtcgcgcgt
     1081 gagcccgtcg ttgacctccg ccagtacgtc accggcgcgc ttgcccgggg cgatggcgtc
     1141 gtgcagcagc gagcgcgcca tcgccatgat gagcgccgcg gcgagcccct tgcccatgac
     1201 gtcgccgcag acgatgccga ggcgcccgtc cccgaagggg atgtagtcgt agtagtcgcc
     1261 gcccatctcg ccgaggaact cgatgcgctg ctcgagcgcg atgccgggga cctcggggat
     1321 cgtggcgggg aggaggtcgg tctggatgcg gcgcgcgcgc tcgatctcgg ccgccacctt
     1381 gagctgggcc gcgcgcagtt cgtcgtgcag ctccacggac gcggcgagct ccttgtggaa
     1441 gctgtcgcgc tccgcgtcga aggtgacctt ctccctggcc cgcttcacgt agatgtcgta
     1501 ggagtagtag atgaggtaca gcggcgggat ccccaggagc atgccgggca cccccagcca
     1561 gaggtagagg gccgccacca gcagcgccag cgtggagccg gcgaggtagc cgggcagagc
     1621 ccactggtag ttccgccgcc agacccgcac ggcgcccccg tcctgcgtcg agagcgcgat
     1681 caccccggcg agcagcgcgg tgtgggcgag gaagtcgacg atggtcgcgc cgaacgtggg
     1741 aaggaccttg gtcatgaagg cgccgccctc gggcgtcagc gcgaggtaca gcaggcccgc
     1801 gaggtacgtg gacacgacgt agttgccgac gttgaaggcg atcttgacgg ggtcgcgggt
     1861 gacccacgcc gcggtgatcc cgctcacggc ggcgaccgcc atcgccggcc acaggccgag
     1921 ggcgatgagg caggcgagca cgggcacgaa cgacagggtc acgtcgccca cccagggcag
     1981 cttgatctcc cgcaggtcgg tgagcgtggc gaggatcatg aaggccacga ggttgagcgt
     2041 gaagggggcg ggctcgtcca gggcggcggc cacggagacg cccgccgccg ccgcgcacac
     2101 cagggccacg tacagcgaga cggccgtgcg cctgcgcacg gccgcctcgt cgtgaccgat
     2161 ggccgcaggc gtctcggcgg cgctcactgc ccttcgatga ggacggtcca ccggaacttc
     2221 gcccactcgt actcggtcca ccggaacttc gtcgcctggt agtcggtcca gcggaacttg
     2281 gtccagcgga acttggtcca ggccacattg gtccagcgga acttggtcca gcggaacttg
     2341 ttccaggaga cgcccgtcca gcggaacttg ttccacgaga cccccgtcca gcggaacttg
     2401 gtccagcgga acttggtcca tccgtacttc gcccaggcga ccttgtccca ggcggcgtag
     2461 tcggcgtcgg tgaggacggt cgttccgtcc ccgacatcct cggagagggc atagccgtcc
     2521 gcctgcgcgt cgctctccag agcgccggcg gcgtcgatca ggccggcgcc ctgctcatag
     2581 cggtcggtgt cgggcagggc cttggcggtg cccatcatgc gcaccttgac gtcgtccggg
     2641 ctcaggtccg ggtcctcgcc gatcatgagg gccgcgacgc ccgccccgac cggcgccgag
     2701 gtgctgctgc cggagagcat caggtagctg ggttcggcgt ccgcgggcgc gcccggcacg
     2761 tagctggcca ccgggatcag gttctgcggg aactcgctgt cgatgtagga gtccttcacg
     2821 cgcagcgaga tcagccggtt gccgggggcc accacgtcgg gcttggcgta ctcgtcccac
     2881 agggtggggc cgacggagct gtagaaggtg accgcgtcgt cgtcgaggga cgcggtctgc
     2941 cgcgtgtcgg tggcgcccac ggtgatcacg taggggctgt tgccggggga gaggatgccg
     3001 ccggggccga actcgccctc gttgcccgcc gcgcacacca cggtgatgcc gttcttccag
     3061 gcgagctcga ccgcccgcgc gatcgggtcg aagtcggccg gtgcgaccgg gttgctcccg
     3121 agcgacaggt tgagcacgcg gatgttgtac tcgtccttgt gggcgatgac ccagaggatg
     3181 ccggacatca ccgaggacgc gcgtccctgg ccggtcttgt ccaggacctt gacgccgatg
     3241 atgtcggcgg ccggcgcgac gccgcggaac tgcatcgtcg cgtacccgtg ctggtcgagc
     3301 ggcagggacg ccgtgccgtc gccggcgatg aggccggcga cgaaggtgcc gtggccggcg
     3361 tcgtcgtacg gcgccttgcg attgtggacg aggtccttcc agccgacgat gcggctgctc
     3421 tggaggtcgg aggtggtggc gatgccgctg tcgaggatcg ccacgccgac gccggcgccc
     3481 gaagggcctc cggccgcggg cgccgcgacc ctgtccaggc cgatcgccag gttggtcacg
     3541 tccatcgagc tcgcgtagtc gaacccgaac accgggttgt ctgccacgat gagagcgacg
     3601 gcgtcgcttg cggcgagcgc gtcgatctcc gcctccgtca ggtacgcggc gacggcgtcg
     3661 aagccggcca gggtcgtggt ctcgacgccg ctgggcacgg tccgtccgac cacgccctgg
     3721 gctccgggat ccgtgtacac gatgacggga acggcgtcgc tcccgccggt ggctgcgagg
     3781 acggtcgtga cgctgtcgtc gatcttgacg gcctcgtcac ttgcggcccc tgccggcgag
     3841 ggcacggcca gcaggcagag cagcgccatc gcgaggacca gatacttggc tcgcatcttc
     3901 atcgcctcca cacccgtgct ccttgtgcct gggatccgcg agaggcgctg gtcgctggaa
     3961 gcgagatccg cgactgcgag cgtcgggttc ggcaaccgag ctatcttgat catcccaaga
     4021 cctctggctt tgcgtccctg cctcacgaca ggtttgcctt tgtcgttcat cgtcccctat
     4081 cggcctctcg cgcgcggaag ttgagcactt tcgtagacgg attgacacag caagtgtcgc
     4141 cggtgtggct acgggagcgg cggacgcccg gcccgcctcc cttttcgggc cgtgacggtc
     4201 cgattcgtac ccttggggtg atagactgcg gtgacaacac ccagagaccg ggggagccga
     4261 cgcgccaggc ggccgcccac ggtgtcgaag ccgcccggca ccattcctcc ggcacgatca
     4321 cgaggcagtc gcgcgcgcag tgcacgccgg cgcgcgacga caggagggag gagattggat
     4381 gagccgtacg ggtggcctgt acaccgctgc tcagaggcaa gtgatcaatc agatgctcag
     4441 gctggcggcc agcgacgaca agcgcaagat cgtcaaggcg ttccggctcg ctgagaagat
     4501 cacacccgac aaacacaaga gctcagtgcg cttcgtcacg gagaaggtcc aggatgacca
     4561 cccggcgctg ttgatcgcca agcacgtcac ctcgaagctc agcccgcgtt gccgcgatgc
     4621 ctttatcgaa tgccttatcg tgaacactct tcttcgcggc gtcgacaaac ggatgagcct
     4681 cgtggagtcg accggcaaca acgcgccgac gacgatcctc atgagcccca ccatgcgctg
     4741 caatctcacc tgcgagggct gttacgcagc ggaatactcg cccgaccagg acctcgaccg
     4801 cggtctgatg cagaagatcg tggacgaggg caacgacatg ggcgtctacc tgttcacgct
     4861 gctcggcggc gagccgttca tgtgggacga actgctcgac ttcgcggcgg agaacaagga
     4921 cgcctacttc caggtgttca ccaacggcac gctcctcgat cacgacacca tcgacgagct
     4981 tgccagggtg ggcaacatcg ctcccatgct gagcctcgaa ggctcacccg agctcaccga
     5041 ccatcgtcgc ggcgacggtg tgcacgagca ggtcatggag atcatggacg agctcggcga
     5101 ggcgggcgtg ctcttcggct actcctgcac cgtcgctcgc aacaactggc gggccctcgt
     5161 gagcgacgcg ttcgtcgacc cgctcatcgc caagggcgcg ctcctggcgt ggcacttcct
     5221 ctacatgccg gtcgggcgcg atcccgacac gagcctcatg ctcacggccg aagagcgcaa
     5281 ccagttccgc gtcgagatca accgcatccg cgacaccaag gcgttgttcc cggtcgactt
     5341 ctggggcgac gccccgtggg tcaaagggtg catcgccggc aagcactaca tgcacatcaa
     5401 cagcggcggc tgggtggagc cgtgcatctt cacgcacttc gccaccgaca acatccgcga
     5461 cgtcagcctc ctcgaggcgt tcaactcgga cttcttcaag gagatccgtc gccggcagcc
     5521 gttcaaccac aacctgctca tgccctgcat gtggatcgac aacccgaccc aggcgcgcga
     5581 gatcatggcc gccaccggcg ccaagccgac gcatccgggc ggggacgtca tgatcaccga
     5641 cctgcaggac gagctggaca gctacgcccg gtccgtcgac cgcctctaca gccccatctg
     5701 gagctgcctg agcgcggaga aggaggacgc ggccgacaag cacgccaaga cggccgcgat
     5761 ggtcgggggg acccggcgcg acaagatgcc ggtcggctga cgccggcagc gtgagcgtcc
     5821 gggcgggcgg tggggccgag gggcctcgcc gcccgccctt ggtgtcgggg gtgccggggg
     5881 tgccgggggt gcgccggccg cctgagggaa tcgtggtacc ctgtcgaaac ctgaagtcag
     5941 ccggtactgc ctatggcgag acctcgtccc cacaagcgcg ccgcgcgcct cgcggccatc
     6001 ttcaccctct ccgtcctcgg tgcggtcgcg gctgtctggg cggcgccgcg cgtcagcgga
     6061 gcggtggagg cctggatgag cacgccgccg gtggagctgc ggaccacgaa cctcggcgcg
     6121 gcgctcccgg tcgtccctga gaccggcgcg accggcgtga ccggcggccg cgcggcggcg
     6181 gcggcgggcg acggctctgt tgcgaccgac cccgccgcgt cgggagacac cctggcgccg
     6241 gccgctccgg ccgccgcggc gaccgtcgac gccggcatgc gcttcaccat ggccggcgtg
     6301 cgctgcgcgc cgccggcgcg cgccggcgag gtgcgcgtgc ggctgcgcac cagcgaggac
     6361 ggacggtcct ggagtgcgtg gtacgcggtg acgctcgagc gggtcgccga ggagggcggg
     6421 gaggagctgg cgttcaccga gcccatctgg accggtggcg ggcggtacct ccaggtggtc
     6481 gcggaggccg gcggcggcga cgagccggtg cccgtccgcc tgcgcgacgt gcgcgtcgtg
     6541 accatcaaca gcaccgagga cgccgaccgc accgcggtgg ccgtcggcgt cctgcgcagg
     6601 gcggccgcgg ccgtcgccgg gctccgcctc acgcccgacg ccgcggcgat gacgaccaag
     6661 cccaagatcg tcacccgcgc cgcctggggc gccaacgagt cctggcgttc cgggtcgccc
     6721 gactacgccg cggtcaagat ggcgttcgtg caccacacgg ccagctccaa cggctacacc
     6781 gccgcggagg ctcctgcggt cgtccgcggc gtctacgcct accacaccag gtcgctccac
     6841 tggagcgaca tcggctacaa cttcctgatc gaccgctacg ggaccatcta cgagggccgc
     6901 tacggcggcg tcgcccgcgg cgtcgtcggg gcgcaggtcc tcgggttcaa caccgggagc
     6961 acgggcatct cggtcctcgg cacgttcagc gattccacgc cgccgtcgcg cacggtcacg
     7021 gccctggagc gactgctgga gtggaagctc gacgtccacc acatcgaccc tctgggcacg
     7081 gggacgctgc gctgcgcgta cggtcagaag tacgccaccg gccagcgggt gacgttcccg
     7141 gccatcgccg gccatcgcga cgccaactac accgactgcc ccgggggcag gctgtacgcg
     7201 cagctgccca ccgtgcgcag ggtggtcgcg cgcacgggcc agcccaagat ctacgggttc
     7261 atcgtcgccg acccctccat cagcccgaac ggcgacggcg tgcgcgaccg caccacggta
     7321 gggttcacgg tctcccagag cgcttcctgg accgtcacca tccgcgatga cgcaggccag
     7381 ccggttcgca acctgagcgg cgagggcacc gaggtggaga cgacgtgggc cggccacgat
     7441 gataccggcg cggcgcttcc ggacggcgtg tacacgctgc aggcggacgc gacgagcgct
     7501 gccggcgtag cgcgacccgc gaccgcgacc ctgcgtctgg acacggtcac gccgcgcatc
     7561 gagagcgccg tcatcgagcc cgaccctttc agcccgaacg gcgacggtca ggcggacgtg
     7621 accaggctcg ccttcgtccc cggtgagtcc ggaacggcgc gggtctcggt gctcggcgcc
     7681 ggcgacgccg tgctgcggcg cgtgaccggc tggacgagcg tgatggcggc cgtccggcga
     7741 gtcacctggg acgggcgcgt ctcgtccggc tccgcgctcg agccggcgcc ggagggcgag
     7801 gcgaccctgc tgctcgagct ccgcgacggc gccggcaaca cgacgagcgc caggcgcaag
     7861 gtcaccctgg accggacttt ggcgcggcgc tccgtctcgc gcacgacgtt ctcccccaac
     7921 ggagacggcg tccacgacgg tgtcaccctg tcgttccggc tcacgcgcgc ggcggaggtg
     7981 acggtgaccg tcgtccgcca ggggtcgacc atgcgcacga tgcgtctcgg caggctcggc
     8041 gccggcaatc acagcgtcac gtgggacggc aagctcggcg ggggaggcac ggccgcaagc
     8101 ggcgcctact cactgaagct caccgcgacc ggcgcgcttg ggagcacgtc cgtcgtccag
     8161 ccgctcaccg tcgacctctc gccgccgagg ctgacggcgc cggcgacggc gtcggtccgg
     8221 tacgggagga cggcgaagat cgcctacacg gtgcgggacg ccttcagccc gaaggtgaag
     8281 gtgagcgcca ccgtcaccga cgcccggggg cgcaccgtgg ccacgctggc gctcggctgg
     8341 gtcaagcagg gggtcgccca ggtctgcgcg tggaagccgc gggcgcgcag gacctacacg
     8401 gtgacgttca aggcgctgga cctgggcggc aaccgccagg ccaagccggc ggtcacgtca
     8461 ctcagagtgc gctgacccgg gcatgcagaa gccccgatcc cgcacccggg accggggccg
     8521 tcttcgtgtg agctatctgc gagccggcgc gccggcgtgc cgggcaggcg ccctgggcac
     8581 gcgcaaggtg cgtacgacca tgacgacgac cgcgaggatg cctaccacca gcgcgatacg
     8641 gaacgcatac agcaccacga ctgctgccca tgcgagaatc tcccagacca tctcggcccc
     8701 ttccgtttcg cgcggaccgc ccgatgcggc cctcatgcat actcttccct gtgcggccgg
     8761 atggtcctat aggggtaatg cctgagatgg tcctcgggtt tgggcgtaga tgcgggggcg
     8821 gatgcagcgc ttccgccccc gcatctcccg gctctcaggc tccgcgtctc aggcctccgc
     8881 gtgctcccgc cgccagcggc agtgcggcat ggggcaggcg gccatgcgtg cggcgtcgca
     8941 gacctcgcag cgctggtcgg ccatgctgac ggggaggtcg aagcagccct cgggcgactc
     9001 gccgaggcgc agcacggcgt gcagcgcgcg cgagaccgcc ttcatgtcgg cgaaggtgtc
     9061 cgccttctcg ggcaggtcga cgaggcccat gcggaccgcg cgcttgcgca cgcggccgtc
     9121 cgggacgcag gcgaagcggt cgagcgcctc gtcgtcccag agcccgtagc ggcgcatctc
     9181 gcggaccgcc cagaaggcgc gcgacttgag cgcgccgccg gaggcggcgt gcagcacgtc
     9241 gaccgccacg tccgggtccg gcggctgcgg cagcagccgg ccccaggtgt cgcgcgtctc
     9301 cgccaggcgc acgaagtcga tgatcttgcc cttgctgtcg ttgcgggaga ccggagggtc
     9361 cagggcgtag cgcttgaggt cgaactcgcc ggcgaacaac gcgtcgacga cggcggcgtc
     9421 ggcgaaccgc ctccagtcgg cgtcgacgac ggagtccggc gccggctggg cggcgagcgc
     9481 tccgtagcag aggagcgtga agaagaggcg cggctgctcc gcctcgtcgg aggagaacct
     9541 gtcgcgctgc tcggcgtcga ggatctcgga cggcagcttc ggggcggcga ggaacgcctg
     9601 ccccaggaac gccacgttct cgcggcgttc gcggttggtg agcgtggtcg attcgttcac
     9661 ggatgccccc acgggtcggg tgatgtaccg agggcggccg gccggcgcgc cgcagaagca
     9721 tgctaggggc gcgccggccg gccgcacagg ctcacgaagc ccgcccgagg gtgaccttgc
     9781 gggtgccgac agggtcacgt tgccgcgccg gcaccgccac gcgtggggcc tccgcgttga
     9841 cggcaaaagc ggactcatat agtctgcttt cagtcggttt gctccgcgcg tgcgcggacg
     9901 gtagtctttg cagtcggcgg ggctcggctc cccagggagg accacgtttc acacccatga
     9961 ccacgatcgg tatcgatatc ggctgcatca gcctcaaggc cgcgttcgtc ggccaggctg
    10021 gcgaccagga cctcttccgc gaacttctct cgcagcaccc ggatctcttc cagacccctc
    10081 gtgacggcct caccgtcgtc ggggaggcgc cggtgctcgt ggcgcgctac cggcgcatca
    10141 agggcggccc cgccgaggcg gtcgacgctc tcctgagcga cgtgctcgcc gtcctgccgg
    10201 agggctccgt gcgcggcgcc cgcgtcaccg ggtccggcgg gcgcctggta ggagcggcgc
    10261 tggacgcctc gttcgagaac gagttcaagg cgatcacgcg cggcatcgcc gcgctccacc
    10321 ccgagacgca gaccatcttc gagatgggcg gcgagacctc caagttcatc cgtctcgagg
    10381 ccagcgagat cggctgcgcc ggcatcaccg actaccagac caacggcgac tgcgccgccg
    10441 gcacgggctc cttcatggac cagcaggcca gccgtctcct ctacgacatc gaggacgtcg
    10501 gcgacatcgt gctggacgcc ggcaaggccg ccagcatcgc gggccgctgc agtgtcttcg
    10561 ccaagagcga catgatccac gcgcagcaga agggctatca gccgcccgag gtcttgcgcg
    10621 gtctctgcga cgccgtcatg cgcaacttca agggcaccat cgccaagggc aaggtcgtgg
    10681 agccaccggt cgcgttcatc ggcggcgtgg ccgccaacga cggcgccgtg cgctcgctgc
    10741 gcgaggcctt cgcgctggac gacggccagc tgttcatccc cgagtaccac gccagcatgg
    10801 gggccgtcgg cgcggcgctg atcgaggccg acctcgaggc cgcgcgcgac cgcgccctcg
    10861 cggggctcga cacggagcgc ctgcccgcgg ccgccttccc ggtgacggag ccgctgtcca
    10921 tggaacgcgt cgtcctgctc cgcgacatgg ccaagccgta cgtgttcagc gacgcccgcg
    10981 aggtcgtcga cgcgtacatg ggcatcgaca tcggctcggt gtcgaccaac ctggtcgtca
    11041 tcgacgacga gggcgaggtc atcaaggaga tctacaccaa gaccgacgcg cgcccggtgg
    11101 aggtcgtgag caaggggctg gcggacatcc agagcgagat cggcgcgcgc atcaacctgc
    11161 tgggcgtcgg caccaccggc tcgggccgcg agctcgtcgg cgagctcacc ggggccgaca
    11221 tcatcaccga cgagatcacc gcccacaaga cgggcgccga cttcatcggc cgcaggatcg
    11281 gcaagcagtg cgacacgatc ttcgagatcg gcggccagga cagcaagttc atcagcctgc
    11341 aggacgggat cgtcgtcgac ttcgcgatga acgaggcctg cgccgccggc acggggtcgt
    11401 tcctcgagga gcaggcggag aagctgggca tcaacatcat cggcgagttc gccgagctgg
    11461 cgctcggctc gccggccccc atccgcctcg gcgagcgctg caccgtgttc atggagcgcg
    11521 acgtcatggc ctaccagcag cgcggcgccc gccgcgacga cctggtcggc gggctcgcct
    11581 tcagcatcgc caccaactac ctcaaccgcc tcgtgcgcga acgccacgtc ggcgacgtga
    11641 tcttcttcca gggcggcacg gcctacaacg actccgtcgc ggccgccttc agccagatcc
    11701 tggacaagga gatcatcgtc ccgccgtaca acggggtcat gggcgcgctc ggcatggcgc
    11761 tgctggcgcg cgagcgcgtc aagcgcaccg gcgagcccac cacgttccgc ggctgggacc
    11821 tcgagcaggt cgactacacc gtcgtcgact tcgtctgcaa ggcgtgctcc aacgagtgcg
    11881 acgtgcgcca gttcaccatc gagggcgaga agacgtactg gggcgacaag tgctccgacc
    11941 gctaccgcaa gccggccaag gtcgacaagg agccggtgat cgccgacctc gtcgccctcc
    12001 gcgaagaggc cctcatggcg ccctacgagg cggcgcgggc gcgcctgccc gagacggcgc
    12061 ccacggtcgg cttcgcgcgc gccatgtaca cctacgaccg cctgcccttc tgggcgacct
    12121 tcttcgccga gctcggcctg cgcccggtgc tctcgccgga gagcgacaag cagatccgcg
    12181 aggccggcgt ggcgctcacc gtcgccgagc cgtgcttccc catccgcgtg gcgcacggcc
    12241 acgtgcagtg gctcctcaac gagggcgtgg actgggtctt cgtgcccaac caggtcaacg
    12301 aagagaccga gttcgagcag tacaactcgc acgcctgccc ctggggccag acgctgccct
    12361 tcgtgatcaa gacggccccg ggcctgcagc cgcacaagca ccagatcctc gacccgcgcg
    12421 tgcgcttccg cgacgggcgc gaaggcctcg cgcgggactt cgccggggtg gcgcagaagc
    12481 tcggcgtgag cgcctcgaag ctgagcggcg ccttcgccgc cgcctaccag gcgcaggacg
    12541 cgttccgcga cacgctgctg gccgccggcg cggaggcgct ggagcgcctc gagctggccg
    12601 gcgagctcgg ggtcgtcctc atcggacgcc cgtacaacat gtacgacaag ggcatcaaca
    12661 tggacatccc gcgcaagctg cgcaagttct acggggtcaa cgtgatcccg ctcgacttcc
    12721 tgcccatccg cggcatcgac atcgacccca tcgtgcccaa catgtactgg aactacgggc
    12781 gcaagatcct gcagacggcg acgttcgtgc gcgacaagga gcggctgcac ctcatctaca
    12841 tgaccaactt caagtgcggc cccgactcgt acatcaagca ctacgtgcgc gaggcgtcgg
    12901 gcaagccctt cctcaccctg cagttcgacg agcaccagaa cgacgccggc gcgatgaccc
    12961 gctgcgaggc gtacctcgac agcaaggggt tcctccgctg gtggtcgcag gacgcggccg
    13021 acgaggccgt cgtgtgccgc gaggaggtgg ccggtgtcgt cgaataagcc ggccggcggc
    13081 gcggccgcgc gcgagggcag cccgcaggac ccccgcatcc agcaggcgat cgccgacttc
    13141 gggctcatgg gccgccgcct gtacctgccg cagatgccct acggcggctc gtacctgctg
    13201 gcggcggcga tccgctccat cggattcgac gcgtgggtca ccagcgactc cgacgaacgc
    13261 acgctcgacc tgggcgggcg ggtcacctcc ggcgacgagt gctacccgca gaagatcacg
    13321 ctcggcgact tcctgcgcat catcgaggac gagggccgcg acaacgtcgc cttcctcatg
    13381 ccgtcggcca acggcccgtg ccgcttcggc cagtacctgc acctcgtgcg cgccaagttc
    13441 gacgagctcg gctacgacga cgtgccggtc atcagcatca cctcgagcga cgggtactcg
    13501 tccatcggcg cctactccca gaacctcatc cgcaccgcct ggcgcgcggt gctcgcccag
    13561 gacatcctga tgaagctgct gctcaagacg cgcccctacg agcgcgagaa gggcgcgtcg
    13621 gacgcggcct accagaccag cctcgaggag gccggcgagg cgatagcgcg cgtcggcatc
    13681 tcgcacaagg agcagctcgc ggggctcatc cccgtgatgg agcgcgcccg cgaccgcttc
    13741 cgcgccatcc ctgcggacta cgacgccaca cggccactca tcggcgtcgt cggcgagatc
    13801 ttctgccgcc agaacacgtt ctccaacttc gacctcatcc gcgtggtcga ggagcagggc
    13861 ggggagtgct ggctgtcgga catcggcgag tgggtgtggt acaccgacga cgagcaccgg
    13921 cgccgccttc gcgacgagcg ccgccgcgtc agcaaggacg gcgccatccg cttcatcaag
    13981 aacaagatca tgcgcaggga cgagcacgcc atgtacgggc ccttccacga cgacttcgtg
    14041 ggttacgagg aaccgcagga cgtgcgcgac gtgctcgaga tgagcttccc ctacctgccg
    14101 tacacgggcg cgctgggcga gatggtgctg agcagcggca aggcgatcta cctgtacgag
    14161 aagggcgccg acggcatcat cgacatcagc cccttcacgt gcatgaacgg catcgtgacg
    14221 gaggccgtct acccgagcct ctccaaggac ctcgacgacc tgccgatccg ggtcttctac
    14281 ttcgacggca cccagggcga cctggagcgc gacgtcggga tcttcatcga gctcgcgcgc
    14341 acctaccagc gccgcaagca caagacccgg cgctaccccg cgtacttctc cgtctgagga
    14401 cggttcgagg caggggaagg gcccctcgcc ctgttccctg gcgggttggg gccggaacca
    14461 ggggggcgag gggcccttgt ctaccgggac gcgacggggg ggacgccgcg ctcgggacgg
    14521 ggagctctag gtgtctcggg tcagcgaccc atccagcggg tgccggcgga gacgtcgaac
    14581 atcgtgtcga tgggcacctt caggtgaccg cgcgcgtcgc ccgagggcgt gctgacggcc
    14641 gaaccggttc cctcgaaaaa gcggccgatc gagcggaaga tacgattctg catgctactc
    14701 accccttcag gctcagagtc cgtcgcggtc cgcgccgaac gtcatgtcgt cgagcatgaa
    14761 gtcccggccg cgaagcgggc cacggactcc ctgaggactg gtcgccacgc tcgtgacggc
    14821 gacctcgata atgcgcttga tgccggcaag acgctcagcc atggcgaccc tccttctcta
    14881 aaaatacctt catattgtgt atctcggcac aaagtgtatt tcctgaagtg ctttctgtca
    14941 aacgtgtcaa ttgcgccggc gacgtggcga aggtcagcgg gggagacggc gcttcagcgg
    15001 gcgcaaagca gctgcagcct gagtgctcac gcgacgtcca cggtgccgcc ggagagcgtc
    15061 acgaggccgt cccggcgcag cccgtccacc acctccgccg cctcgccggg cgcgagctcg
    15121 agcgccgccg ccagctcctc gagcggctgc ggcccgtgcg cgagcagccg ccggagcgcg
    15181 cgcgcccgct tctgccggcg cgagccttcg aacgcgccct ggcggctgcg cgaggcgatg
    15241 ccggtgctgc gggcggtgag caccagcgag ccgtagtcca tcagcgcgtt gtgccagtcg
    15301 cggctgcggc cgcgcggcag caccgtgtcc gccaccgcct gcaggtcccg gtcgccgagg
    15361 tcctccggca agccgagctc gtggctgagg acgcggcgca cgttggcgtc caccgcggcc
    15421 aggtcgtcgt tgtgcgcgaa gacgaggacg gcgcgcgccg tgtacgggcc gacgccgggc
    15481 agcctgcgca gcccgtcgag cgaccgcggc agctcggcgc ggcgctcagg gggcgcggag
    15541 gcgacggccg ccgccgcgca ctcctgcagc cgccgcgctc gggtgttgta cccgagtccc
    15601 tgccagcgcc gcagcacgtc cgccagcggc gcggcggcga ggctctcgag gtccggccac
    15661 gcctccagcc actcgacgaa ccgcggggac acgcgcggga cctgcgtctg ctgcagcatg
    15721 acctcgctca ccaggatcgc gtagggatcg gcggtgcgtc tccacggcag gtcgcggcgg
    15781 ttctccgcgt accaggcgag cagccgggtc tgcagcgccc gcacgacgcg gacggagggc
    15841 aggcccaccg tctcgccgct catctgggga ccgaagcggt gaccatcggc gagagcgtag
    15901 cagacgggcg gggtgcgtcg aagcccgagg ctcaggcctc ctcgtcgacc agcttctccc
    15961 aggccgcgta ctcgcgctgg aggcgcttct cgacagcgct gcgctcgtcg agcagccggt
    16021 tcagcacgtc gcggtcggcg taggtgtccg gctcggcgag ctccgcgtcg atctcgccga
    16081 gccgtctctc cgccttgagg atgccggcct ccgcgccgcg caggcgggcg cgccggcggc
    16141 gcgcctcctc ggcgctctcc ttgccgcggg tgacggcggc gccggccggc gccgccgtgg
    16201 ccgcccttct gccgccgcgc ggcggttccg gcgtcgcggc cggcgcgatc cccagccgtt
    16261 cgttgcgcgc ctcccagtac cgagccgcgg agaggtggtt gaggaggcgc ccgtcctgga
    16321 tctcgagcac gcgggtggcg acccggtcga tgaggtgccg gtcgtgcgtg accaggagga
    16381 tcgtgccggt gtagtcctcg agcgcgtcgg cgagggcctc gaccgacccc atgtcgaggt
    16441 ggttggtcgg ctcgtcgagg agcaggaagt tggcgtcgcc gacgagcagg gtgagcaggc
    16501 gcaggcggct gcgctcgccg ccggacagca tctccacccg cttctcgacc tcatcgggac
    16561 cgaactggaa gcggcccagc aggccgcgag cgctctgctc gtcctggccc acgagcggca
    16621 ggaccgactg caggaccgtg tcctcctcgc gcagctcccg gccctgctgc gagtagtagg
    16681 cggcgcgcac gttgtgcccg aggtgggagg cgccggccgg caggctgcgg aggccggcga
    16741 ccgtctcgat cagcgtcgtc ttgccggagc cgttcggccc cagcagggcg accttctcgc
    16801 cacgctcgag caccacgtcc acgtcgtgca cgagcgtgtg ggcgccggcc gcgtcgtcgg
    16861 cggagccgac gccgatcgcc ggcgccttga gctcgagcac gacacgggcg gagggcttgg
    16921 gctgcggcag ccccagcttg aagctgcgct tctgcctggg gggctcccgc agcccggcct
    16981 tgatgcgctc gatctgcgtg agcttggcct tggcctgctt ggccttgtcc ttcttggcgt
    17041 ggaagcggtc gtagaaccgc tgcagctgct cgatcttctc gaggtcctgc tcgtattccc
    17101 ggcgcatgag gtccaggcgc gcctcgctct cacggaggta ggtggcgaag tcgccgccct
    17161 gggtctcgac gtgctcgtgc tccaccgcga ggacgcgccg cccgaccttc tcgagcaggc
    17221 ggcggtcgtg ggtcaccagg aggaacgcgc gttcgtagcc ggagaggaag ccctcgagcc
    17281 actccaccga gtcgatgtcc aggtgattgg tcggctcgtc gagcagcagc aggtcggcgt
    17341 cggcgagcag cgcgcgcgcc agcgtgacgc gcgtgagctc gccgccggac agcgagagca
    17401 ggttgcgctc cagcatcgcg tcggggaggc cgaggccctc cgccacgccg ccgaggcggt
    17461 cgcggtagcc gtagccgccg gccgcctcga agcggcgctg cagctgctgg taggtgcgca
    17521 tcgtgtcgcg cagctcgggg ctgtgctcgg cgaggccgga gaggcgctcc tcggtacggc
    17581 gcagcgccgc ctcgagctcc acggcctcgc cgaaggcctc gagcaggtac tcgtgcacgg
    17641 ggcggtcgcg ctcgagctcg ggggtctgtt cgtggagcca gatgcggcgc ccgcgggcgc
    17701 gggcgatcgt ccccgagtcg gcgtgctcgt cgccggagag gagacgcaga agggtcgtct
    17761 tgccggtccc gttgcggccc accagcacgg cgtggtcgtg ctggtcgatg gagaacgaga
    17821 cggtcgtgaa cagccgctgg ccggcgtacg acttctcgac gttgttgaag gtgacgatgc
    17881 tcatggcgtg gccgccggcg gctttcggca gcgggcggga gcggcaggat accaccggcc
    17941 cgccgcgacc ggcaacgtgc ggcccccgcg gcgagcctgc tacgctgcgc acatccgccg
    18001 agcagcagga ggtgcccaca catgagcgtc ggtcgcgccc aggacatcct gatcggcccg
    18061 cggaccacgg tccacgctct gctgctggca tacccgttcc tggagtccta cctgctcgcc
    18121 cgcgtgcgtg gcttcgagcg gctgcgcgac cggcgcgcgc gcacccggtg ggcgcgcgtc
    18181 atgaccctcg acgatgtcgg cgtgcgcctg aacgtgccgt ggcgccaatt ggtgcgggag
    18241 atcgccgccg aggtggagca gcgcaccggg agaccggcac gcgtggccga cgccccgcag
    18301 cgggtcgtcg acgacggacg ccgcatcgga gagctccgcg ggatcgtcga ggggctggag
    18361 gccggcgcgc ccctgctgga gatggcggcg cgctggggcg ccgccaccgg cgacctcgaa
    18421 cgggccgagg tggacgcgct cgacgccgct ctctccgaag cggcgctcgc cggtcgcgcc
    18481 ggcggcgagc gcgtggcgct ggccgcggcc cccggcgaca tcgcgctcgc ggaacccccg
    18541 ccgggacatc ccctcgagac gctgcggcgg gaggcgcgtc acgtcctgct cctgaccgcg
    18601 ggcctgcgag ccgagatgga ccggctcggc ggctcgccgc cgcgacggcg gtggcagcgc
    18661 gccaagcccc ttgtcgcgcg gctcgccgac aggctctcgg ccgtggagct gcgcttccgc
    18721 cgcgagcagc aggcctggtt cccggccctg gaggtcctcg ggatcgaggg accgcaggcg
    18781 ctgctcgcac cgcgtcaagg cgaggcgctg gagacgctcc gccgcctgcg cctcgcggtc
    18841 gaccgcgacg acgcacccgc ggccgtcgag gccgggacgc ggctcgtcga cgcgctcgag
    18901 gatctgctgg ctcaggatga gcggctgctc gagccgctgg cgctgcggca cctctctccc
    18961 gaggactggg cggcggtccg cgagctcgag gacggcgtcg gatggggcct cgtcgtgcct
    19021 cctccgtggc ctgattcctg acctggacgg ggggcttcgc acgtctccgc gcggggcaaa
    19081 ccggtgtgca tggacggccg gtaccccgga gacggactgc tcgagaccca ggacctgatg
    19141 gcccaggagg gccgccgcgt ggtcgacacc gctcgccgcc gcggcgtcgt gctccggctg
    19201 ctcggcggct tcgccgtcca cgagcactgc ggcggcaaga cggcctgccg ccgcgaccac
    19261 ctcgacctcg acttcgccgc tctgagccgg cagacgcggc cggtggtcga ggtcttcgag
    19321 gaactcgggt acgaggagcg gatccacgtg cggcaggcga cgcggatgat gcaggcgcag
    19381 ttcgtgcgcg agtgcgtgca cggcggcggc ggcgtgccgc gcgcccacgg ggaggaccac
    19441 gtggacgtgt tcttcgaccg gttcaagctc gaccacagcc tcgaccttcg tgaccgcctc
    19501 gcccagcacc cgtacgccgt gccgctgacg gacgcgctcg tgaccaagct gcagatgcac
    19561 gcgccggagc cccgcgacgt ccgcgacgcg atgttcctgc tggcggccgg cgcggaaggc
    19621 ggcgaggccg gcgacgtgga cccgacgtac gtcggcaggc tctgcgcgcg cgactggggg
    19681 ctcttctacg acgtcgcgct cagcctccag cgctgcgtgc gcgcgctcga cgaggccggc
    19741 ctgggtcccg aggagcggga ccgggtcgcc ggcctcctgg cgcgcctcac cggcgccctc
    19801 gacgccgcgc ccaagaccct ggcctggcgg ctccgggccg gcgtggggac gcgccgccgc
    19861 tggtggaacg tcgtcgagga acaggggcgc ctcacggcct gaggctggcg gcccgcccct
    19921 tcttggtgcg cacggcgagc gacatgtcct ccgtgcgccc gccgaactgc tcctccacct
    19981 cgtcgtacca gcgcacgcgc ttgccgatgc gcgcccgcag gcgccagcgc agcgtcttgg
    20041 cctgctcggt catcatgtcc tgcgccagct cgagcgtgcg gcgcacgcgc gcggcgtcac
    20101 gggggctgag gtcgtagtcg gcgacgtggt cgatcacggt gtcgatgttg ttcatcacgt
    20161 cgatgtacag gccccagtcc gcggcgcaga ccccggcgac gtggtgcagg tccagcacgc
    20221 cgggatgtgg ctggaagtcg acgtacacgt ccttgaccag cgtgacgatg tcgtggcagt
    20281 ccttctcgtt caggtggacg atctggagct tgctgaggaa gaggtcggcc gggtcgatgg
    20341 cgtaggtgtt gatggtcaag cggtcgcgga agtcgatctg atgatccatg cgcatcgcgt
    20401 cgaggaagac gtcgacgtgg tcgctgggcg ggacctcggc gtagacgggc aggtcgtcgg
    20461 gggccgcgtc gcccccggcg cccggcgcgg aggcacacgc cgcgccgggc aggaagaact
    20521 gcagctgccc gttgccggtg gccatggcgg cgtggcggtt ctcgatgtac ccgagcccgc
    20581 ggaagacctg gccgacggcg accacctggc gcttgagccc gatcaggtcg atgtcgctgt
    20641 actcgcgctc agcgaactcg aggtcgatgg cgtagtgccg cacggccagc ccgccggtga
    20701 ggcgcaggac gacgtcctgc cgggtgccgg cgtcgatgat cgcgtgcgcc tcggccatga
    20761 ggcgctcgtg ccggcgcagg gtggcgtcga gcgccgcgtc gtgcaggcgg gaggtcgggg
    20821 gggtgtccac gtcgcagcgg gcgtcaggcc ttgaccgccc gcacctcgac cttctcgagg
    20881 tccatctcgc ccagacccat gtcgtgggcg gcctgcacgt acgggatgtc ggcgccggtg
    20941 aggccgaaga gcgtggcggc gtaggcgtcg gcggcgacga tgtcggcgct cgccagcacg
    21001 gtgtcgagcc gctcgacgtc gtcgaggtcg ccgccggtgg gcccgttggc ggtgagcacg
    21061 cgcacggcgt cgacgaccgt gagcgtgggg cggcacacgc tgacgaggtc ggcggtgcgc
    21121 tgcccgatgt tggtgtggat gtcgccggcg tcgaggatga cgccgaggag gttcttgccg
    21181 cccaggctca ggcgcgtgga gccgtggtcc ttggcgatcg gcacgtcgat gagcgcgtcg
    21241 cacgagagca cgtccgtgta gatgggccag gaggacaggt cccggccgtc ggggatgcgg
    21301 aactcgcgga agccggcggg cgacatgatc tgcatctccc cgccggcccg ctccactgcg
    21361 tcctggatgc cgctcacgcg gtacgcctct tcggccgggc tgccgaaggg caggtccatg
    21421 acgcgcaccc ggcgggcctt cgcccgcagg cacagctcga cgagcgttgc cacgacctcc
    21481 ggattggtgg tcgcggcgta ctccggcccg tggtacccgg tgcagatgtt gggcttgacg
    21541 acgacgtcgg cgcccggccg cacgaaggcc tccatgccgc cgagcgcggc gacggcggcg
    21601 gcggtgatct cggcggcgtc gccgccccgg gcgacggaga gcacggactg cccccgcttg
    21661 ccgccggggg aggccggcgg ggaggagagg cggccctcct gcggggggtc ggctgcgggc
    21721 tgcgagccgc agcccgcagc ggccagcgac gctccggcgg ccgccaggcc ggcgatcttg
    21781 aggaactccc tgcgggtaca gtcgcgcatg cctgcgccaa gggtagcagg ccacggcccc
    21841 gaactcacct actgccggtg ggagcagccg ccggcgcgcg tccacgacct agcgacaggg
    21901 gaggagcaca tgaacatcgg cgacgtgttc gccagggcgt gggacctgtg gcgcagggac
    21961 gtcggctggc tgatcctcgc cggtctcgtc gtggggctca tcatggcggt gatcttcggc
    22021 atcgcgttcg cgctcttcgc ggccgtcttc gccggcgccg gcgtcaccat cggcgccgac
    22081 ctcgccaacg acacgacctc gtcgctgacc gggctgggcg cttccctgct ggtcgtcggc
    22141 ttcatcgtct acatcgtcgc catgttcctc atccaggtcg tcgcgatgac gttctacggg
    22201 ggcatgttcg agatggtgat cggcgcctac cggggcgacc gcggcgtgga gttcggggac
    22261 ctcttcagcg ggttccgcaa gatcggggcc tacgccctgt acgccctcgt gctgttcggc
    22321 atctccctcg ggctcagcct cctcaatatc ctcccgctca tcggcggggt catctcgttc
    22381 gtcgtctccc tctggatcac ggtcatctgg ctgtacgtgc tgccgctgat cgccgaccag
    22441 ggcctggggt tcggcgaggc ggcgagtcgc agcaaccaga tggtcaaggg cgccggctgg
    22501 tggtggacgt tcggcatggt ggtcctgctg ggcctcgccc tgatcgcggc ggccgtcgtg
    22561 atcctgctcg tcgcctgggg cgcctaccag ggaagcgagt gggtggggat cgccgtgggc
    22621 atcctgctgt tcctcgtgtt cgccgtcctg ttcccgccct acgccatctg ctacgtgagc
    22681 gtgctgtaca tcgcctccgg cggcgacatc gccgtcgccg cgacgccggc cggcgggctg
    22741 cccgggatcc cgccggctcc tccggccccg ccgacctacg gcactccggc ctcgccgagc
    22801 gccgccggcc ctcccggcgc gacgggggtg acggcgccgg tggtcccgcc ggcgacgggc
    22861 gccgacgcct ggagggcggc ggccgacccc ctcgcgggcc agccgccggc gcctccaccg
    22921 gcgcccgcgc cgtcggcggc gccggccgtg acggcggcag acgaggccgc cgtcccggcg
    22981 ggcgagtccg cggcggccgc ggggcaagcc gccgtcgagc cgcagaccgc gatcacgcag
    23041 gtggacgccg agtcttccga gccgcccgct ccgcctgccc ctccggctcc gccggcgccg
    23101 tctcctccgg gagggggcgt ctgagccgcc tcgcctgagc gtcgagggca cggaggccgg
    23161 ggcgcctggg cgccccggcc tctcgcgttg tggggaaagg tctccggcct cggaatcggg
    23221 cctcttgtaa agtatcttga cagatgtgat tacttcacta tggagaaggg tacacctctt
    23281 ccaggcgccg gcgacctcag aagggagtcg tggcctcgcc gggcagcgcc ggcgacacgc
    23341 aacgaacagc tggaggagac aatgaagacc cggcacatca tcgccatcgt ggtagctctg
    23401 atggtggtcc cgctcgtcct ggcagcgtgc agcagttcta cggaagaagc tgcgtcgccg
    23461 accccctcgc cgacgccgtc catggtcctg aaggacatcg tcgacaccgc ggtcgaggca
    23521 ggcgacttca cgacgctcgt ctcggccgtc caggccgccg gtctcgagga gacgctgcgc
    23581 ggtgaaggcc cctacacggt cttcgcgccc acagacgacg cgttcgcggc ggtccccgcg
    23641 gagacgctgg acgcccttct ggccgacccc accggtgcgc tgaccgacgt gctcacatac
    23701 cacgtggtcc ccggcaaggt catgtccacc gacctgagtg acggcatgca ggcgacgacc
    23761 gtcaacggcc aggcgctcga catcaccatc gcggaagacg gcaccgtcat ggtcaacggc
    23821 gcgacggtga tcaccgccga catcgagacc gcgaacggcg tcatccacgt catcgacacc
    23881 gtcctcgtcc cgagcgagtg acgttccgtc caacgacctg atccacgggg cggccggcca
    23941 cagtgccggc cgccccgtct gcgtacgcgg tcacctctgc ccgcgtgcac cggtgtgcag
    24001 caggcgcgcg agctcctgcc acacgcgcgg gtcctcctgg cccatcagcc acgcgtacac
    24061 gccgcggatc cgcgggtgtc gcgagagcag catgcgcgcc ttggcgcgca cggccggcgc
    24121 gtcggggaag aaggcctcgt gactggcgcc gtcgcggcgg taggcgagcc gcagctcgcg
    24181 cgacgcggtg cgcgtctcgc gcgggtcgag ctccgcgcgg atcgcgcgca cgtcgtccca
    24241 gacgaggtcg gtcgtccccg acccacccca gtcacgcccg tagaacccga gccccatgac
    24301 gatgcggcgc ggcgcgacct gcgtctccgc gaagctgagc acgcggtcca tccagtccgg
    24361 cggcgagagc ggccccgggc cggaccacga tcccgagtag ttgtagctca tgacgcggaa
    24421 ctggtcgacg gcgcggccga gccgcgccca gtcctgcccc cgcgggccgt cccagccgcc
    24481 cggctcgctc agcttgggat agacgtccac ggcgatcaga cggtcgtccg cgtgcaggcg
    24541 ggcggcgagc tcctccacga acgccgagaa cgcgtcacgg cgcgaaccct tgaccgcctc
    24601 ccagtccacg tcgacgccgg cgacgtcgta ctcacggcac cagtcggcga cggcctcggc
    24661 gtgccgtcgt cgcgtcgccg cggtcctgag gatcgtggcc gagatcgagg ggtcgaaggc
    24721 gtgctccgcc tcgtcgtagt cggtcaccgt gacgaagacg cggcagtccg cgtcccgcgc
    24781 cgcctcgatg aaccccgtgt cgaggcgcgg cgcgaccacc gagccgtccg cccgggactg
    24841 cagccagtcg gcgctgacct cgtcgagcgc gcccgcgtcc accgccgtct ccagcgaacc
    24901 gcgatctccg aaggtccagg cgccgatgag gtacgggccg tgcgggcggt cgtcgtcgag
    24961 cgccgtggcc agcgcgaggg ccgc
//
LOCUS       DASUMX010000103         5765 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_603818, whole genome shotgun sequence.
ACCESSION   DASUMX010000103 DASUMX010000000
VERSION     DASUMX010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 5765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5765
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_603818"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1665)
                     /locus_tag="VFZ86_05025"
     CDS             complement(<1..1665)
                     /locus_tag="VFZ86_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013735543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching N-terminal
                     domain-containing protein"
                     /protein_id="HEX5641688.1"
                     /translation="MSPEVKILDGNTFVVSDDRGDIEASLTDPTGLFSFDTRFLSRWV
                     LTVDGQRLNPLSVDDLQYFETRFFLVPGTGTVYIDAKTSVIRQRAVGGGFHEELTILN
                     HDEKAIDVEARVEVGSDFADLFEVKDALKKQGEYYSRVDDGRLVLGYRRDTYVRETWV
                     SATAACAIDEHGLTFSVHVEPHGEWTTDLHVTLGRGVAGTSVAAPKYERHAKKARPNM
                     ERSLEKWLEEAPTLACDWDPLKRTYQRSLVDLAALRFSPVSLPGHYLPAAGLPWFMTM
                     FGRDSIFTSLQALPFTPELARTTLVALGMWQGSRRDDFRDEDPGRILHELRFGEMTAF
                     EERPHSPYYGSVDATPLYVVLLDEYERWTGDTRLVKALEQKARAALAWIDDYADLQGN
                     GYIAYQRRNEKTGLENQCWKDSWDSISYRDGRLPGFPRATCELQGYAYDAKVRGARLA
                     REVWRDPAFAERLEKEAAELKRRFNRDFWVEDRQHYALAIEADGSKVDALASNNGHLL
                     WSGIVDKAKAKAVARHLMSPAMYSGWGVRTLAVGEARYNPIGYHVGT"
     gene            complement(1662..2033)
                     /locus_tag="VFZ86_05030"
     CDS             complement(1662..2033)
                     /locus_tag="VFZ86_05030"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HEX5641689.1"
                     /translation="MTEPTRAFLERLAEQQQPFLKSLTGVVRLDLVDAERTEHWYLTI
                     RKGDVTVSHEGAEPDCIVSADLSTFDAIVSGEMNAMAAVLRGAVDVRGEFRFLTALRQ
                     LFAGPTKAPDAPTAGYARRQS"
     gene            complement(2069..2836)
                     /locus_tag="VFZ86_05035"
     CDS             complement(2069..2836)
                     /locus_tag="VFZ86_05035"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017739787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX5641690.1"
                     /translation="MRLEGKRALVTGSSSGIGEAIALRFAREGADVAVHYHREEDEAQ
                     RVAAEIQKMGRQSVALGANVGVAAEAEKLVRDAHAALGGLDILVNNAGVEIREPFVEV
                     SEQHYDLVLDVNLKGAFFAAQAAAKLMIAAGTGGRIVNVSSIHEDIAFLQYATYTASK
                     GGMRMLTRTICQELAPHGITVNDIAPGAIATPINKRTLGDGELLHALQEVIPSGRLGE
                     PDEVAGVAVFLASDEAAYVTGSTYYIDGGMARWNKGL"
     gene            complement(2858..3730)
                     /locus_tag="VFZ86_05040"
     CDS             complement(2858..3730)
                     /locus_tag="VFZ86_05040"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007449318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX5641691.1"
                     /translation="MRSAEFEVAVGSGAGLGEGPRWDAATQTLLWVDIPGQCVHRYDP
                     QTGEDSAQPVPGVVSLALPRRRGGVVVGLPDGVHLLDGDRSPLIAAIESERGDTRTND
                     GACDSAGRLWVGTMALDERSPIAALYRVDSHLGVTTVLTGTTISNGLGWSPTGRDFYF
                     IDSPTRRVDVFDCDPATGALDYRRPFAAVEVEGAVPDGLAVDAEGGVWVALHGGWGLR
                     RYSPDGELAAIVDLPVARVTSCCFGGEDLRDLYVTTRREGLSASELAAQPLAGALLRL
                     DAGVAGLPTYAFAG"
     gene            complement(3750..5438)
                     /locus_tag="VFZ86_05045"
     CDS             complement(3750..5438)
                     /locus_tag="VFZ86_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017301588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5641692.1"
                     /translation="MKVIIVGGVAGGASCAARLRRLDENAEILMVERGPYVSYANCGL
                     PYYIGGVIEQQANLLVATTDTFRDMFAVDARTNCEVVGIAAGERTVQLKDRTTGEVTT
                     ERYDKLVISPGAAPVRPPLPGIDLPGIFGVRTVPDAGQIRAWIDERRGRSAGMDSYTG
                     FQTRRRELRAVVVGGGFIGIETAENLVGLGFEVTLLQRGNQIMGPLDPEMARYVERHL
                     GKHGIEVLLDASASGFREAADGSLEVLTGEGPAYPADIVILGIGVRPETQLAEMAGVE
                     VGALGGVRVDDQMRTSDPDIFAVGDAVEKRDCISGEDCLVALAGPANRQGRIAADVIA
                     GRDSRFRGTQGTAILGVFGAAVAWTGMSEKALQRLGDDDYEKIYLYPNSHAGYYPGAS
                     LLAIKVIFRKSDGRLLGAQAVGREGVDKRIDAFAMAIQLGGTVYDLEECELCYAPQFG
                     MAKSPANFAGMVGANVLRDDMPIVHWDGLGDGFLLDVRHPEELAVEQLQGVVNIPIDE
                     LRGRLDELPRDREILVICRSGQRAYYATRILVQNGFTARTVAGGMLSHEIFATL"
     gene            complement(5530..5697)
                     /locus_tag="VFZ86_05050"
     CDS             complement(5530..5697)
                     /locus_tag="VFZ86_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641693.1"
                     /translation="MRTLMVVLMVIGSILLAFLLAGILFANLRFFIWIAIIGLVIWGV
                     YMLVTRPWRYS"
BASE COUNT          853 a         1998 c         1973 g          941 t
ORIGIN      
        1 ggtgccgacg tggtagccga tcgggttgta gcgggcttcg ccgacggcaa gcgtcctcac
       61 gccccagccc gagtacatcg ccggtgacat gaggtgtctg gcgacggcct tggccttcgc
      121 cttgtcgacg atgccgctcc acagaaggtg gccgttgttg gaggccaagg catcgacctt
      181 gctgccgtcg gcctcgatcg ccagcgcgta gtgctgccgg tcctcgaccc agaagtcgcg
      241 gttgaagcgg cgtttgagct ccgccgcctc cttctcgagc ctctcggcga aagcggggtc
      301 gcgccacacc tcacgcgcca atcgggcgcc gcgcactttg gcgtcgtagg cgtagccctg
      361 cagctcgcag gtcgcgcgag ggaagccggg gaggcggccg tcgcggtacg agatcgagtc
      421 ccaggagtcc ttccagcact ggttctcgag gcccgtcttc tcgttgcgcc gctggtaggc
      481 gatgtagccg ttgccctgca ggtcggcgta gtcgtcgatc caggccaggg ccgcgcgcgc
      541 cttctgctcg agcgccttga cgagtctggt gtcacccgtc cagcgctcgt actcgtcgag
      601 cagcaccacg tagagcggtg tcgcgtcgac ggagccgtag tacggcgagt gcggccgctc
      661 ctcgaaggcg gtcatctcgc cgaaccggag ctcgtggagg atgcggccgg ggtcctcgtc
      721 gcggaagtcg tcacgccgcg agccctgcca catgcccagg gcgacgagcg tcgtgcgcgc
      781 gagctcgggc gtgaacggca gcgcctgcag gctggtgaag atgctgtcgc ggccgaacat
      841 cgtcatgaac cagggcagac ccgctgccgg caggtagtgg ccgggcagcg agaccggcga
      901 gaaccgcagg gccgccaggt ccacgaggct gcgctgatag gtccgcttca gggggtccca
      961 gtcgcaggcg agggtcggcg cctcctcgag ccacttctcg aggctgcgtt ccatgttggg
     1021 tctcgccttc ttggcgtggc gctcgtactt cggcgccgcg acggacgtgc ccgcgacgcc
     1081 acgcccgagc gtcacgtgca ggtcggtcgt ccactcgccg tgaggctcga cgtggacgct
     1141 gaacgtgagg ccgtgctcgt cgatggcgca ggccgccgtg gccgacaccc aggtctcgcg
     1201 cacgtacgtg tcgcggcggt agccgagcac cagccgaccg tcgtcgacac gcgagtagta
     1261 ctcgccctgc ttcttcagtg cgtccttgac ctcgaacagg tcggcgaagt cggagcccac
     1321 ctcgacgcgc gcctccacat ctatcgcctt ctcgtcatgg ttgaggatcg tgagctcctc
     1381 gtggaacccc ccaccgacgg cgcgctggcg gatgaccgac gtcttggcgt cgatgtagac
     1441 ggtcccggtg cccggcacca ggaagaaccg cgtctcgaag tactgcaggt cgtcgacgga
     1501 cagcgggttg aggcgctggc cgtccacggt gagcacccag cgcgagagga accgggtgtc
     1561 gaaggagaac agcccggtcg gatcggtcag cgaggcttct atgtcgccgc ggtcgtcgct
     1621 gacgacaaag gtgttgccgt cgaggatctt gacctcgggg ctcatgactg cctcctcgcg
     1681 tagccggcgg tgggcgcgtc aggagccttc gtcgggccgg cgaagagctg ccgcagcgcc
     1741 gtcaagaacc tgaactcgcc acggacgtcc accgcgccgc gaaggaccgc ggccatggcg
     1801 ttcatttcgc cgctgacgat cgcgtcaaag gtggagagat cggcgctcac gatacagtcg
     1861 ggttccgcgc cctcgtgcga gacggtcacg tcgcctttgc ggatcgtcag ataccagtgc
     1921 tcggtgcgct cggcgtcgac gaggtcgagg cgcacgacgc cggtcaggct cttgaggaac
     1981 ggctgctgct gctccgccag tctctccaag aacgcgcggg tcggttctgt catcgtcgct
     2041 gctccagtct cgtgcgctcg tacgcgtgtc agagtccctt gttccagcgg gccatgccgc
     2101 cgtcgatgta gtaggtgctg ccggtgacgt aggcggcctc gtcggaggcg aggaagacgg
     2161 ccacgccggc gacctcgtcg ggctcgccga ggcggccgct cgggatgact tcctggagcg
     2221 cgtgcagcag ctcgccgtct ccgagcgtgc gcttgttgat cggcgtcgcg atcgccccgg
     2281 gggcgatgtc gttgacggtg atgccgtgcg gcgccagttc ctggcagatc gtgcgcgtga
     2341 gcatgcgcat gccgcccttg gaggccgtgt aggtggcgta ctggaggaag gcgatgtcct
     2401 cgtggatgga cgacacgttg acgatgcgcc cgcccgtgcc ggcggcgatc atgagcttcg
     2461 cggccgcctg cgcggcaaag aaggctcctt tgaggttcac gtcgaggacc aggtcgtagt
     2521 gctgctcgct gacctcgacg aacggctcgc ggatctcgac accggcgttg ttcacgagga
     2581 tgtcgaggcc gccgagcgcc gcgtgcgcgt cgcgcacgag cttctcggcc tccgcggcca
     2641 cacccacgtt cgcgcccagc gcgaccgatt gccggcccat cttctggatc tctgccgcga
     2701 cccgctgcgc ttcgtcctcc tcgcggtggt agtgcaccgc cacgtcggcc ccctcgcgcg
     2761 cgaagcgcag ggcgatggcc tcgccgatgc cgctggaact gccggtcacg agcgctctct
     2821 tgccttcgag tctcatcgtc gtaccgcctt tccgcgctca gccggcgaac gcgtacgtcg
     2881 gaaggccggc cactccggcg tccaggcgca gcagagcgcc cgccagaggc tgcgcggcga
     2941 gctcgctcgc gctgaggcct tctcgtcgag tcgtcacgta caggtcgcgc aggtcctcac
     3001 ccccgaaaca gcagctggtg acgcgggcga ccgggagatc cacgatcgcc gccagctcgc
     3061 cgtccggcga gtagcggcgc agtccccacc cgccgtggag cgccacccag acgccgccct
     3121 cggcgtcgac ggccaggccg tcggggacgg cgccctcgac ctcgacggcg gcaaagggcc
     3181 gccggtagtc gagcgccccg gtcgccgggt cgcagtcgaa cacgtcgacg cggcgggtgg
     3241 ggctgtcgat gaagtagaag tcccggcccg tcgggctcca gcccagcccg ttcgagatcg
     3301 tcgtgccggt gaggacggtc gtcacgccca ggtgagagtc cacgcggtag agcgcggcga
     3361 tcggagagcg ctcgtcgagc gccatcgtcc ccacccagag acgcccggcg gagtcgcagg
     3421 cgccgtcgtt ggtgcgcgtg tctccccgct cggactcgat ggccgcgatg agaggcgaac
     3481 gatctccatc caggaggtgc acgccgtcgg gcagcccgac gacgacgcca ccgcggcgtc
     3541 gtggcagggc gaggctgacg acgcccggga cgggctgcgc ggagtcctcg ccggtctggg
     3601 ggtcgtagcg gtgcacgcac tggcccggga tgtcgaccca gaggagcgtc tgagtcgcgg
     3661 cgtcccagcg cgggccctcg ccgagtcccg ctccgcttcc gacggcgacc tcgaactcgg
     3721 cgcttctcat ctgcgctcct cccatcgtct cagagcgtcg cgaagatctc atgcgacagc
     3781 atacccccgg ccacggtccg tgccgtgaat ccgttctgca cgaggatgcg cgtcgcgtag
     3841 taggctcgct ggcccgagcg gcagatgacc aggatctcac ggtcgcgcgg gagctcgtcc
     3901 agccgtccgc ggagctcgtc gatggggatg ttgaccacgc cctgcagttg ctcgacggcg
     3961 agctcctccg gatggcgcac gtcgaggagg aagccgtctc ccaggccgtc ccagtggacg
     4021 atgggcatgt cgtcgcgaag cacgttggcg ccgaccatgc cggcgaagtt ggcgggcgac
     4081 ttcgccatgc cgaactgggg cgcgtagcaa agctcgcact cctccaggtc gtagaccgtc
     4141 ccacccaact ggatggccat ggcgaaggca tcgatccgct tgtcgacacc ctccctgccg
     4201 acggcctgcg ccccgaggag gcgtccgtcg gacttgcgga agatgacctt gatggcgagc
     4261 aagctggcgc ccgggtagta ccctgcgtgg gagttcgggt agagatagat cttctcgtag
     4321 tcgtcgtcgc ccaggcgctg cagggccttc tcgctcatgc cggtccaggc gactgcggcg
     4381 ccgaagacgc cgaggatcgc ggtgccctgt gtgccgcgga agcgcgagtc acgcccggcg
     4441 atcacgtcgg cggcgatgcg gccctggcga ttcgccgggc ccgccagcgc gacgagacag
     4501 tcctcgccgc tgatgcagtc cctcttttcg accgcgtcgc cgacggcgaa gatgtccggg
     4561 tcactcgtgc gcatctggtc gtcgacgcgg acgccgccga gcgcgcccac ctcgacgccg
     4621 gccatctccg cgagctgcgt ctcgggacgc accccgatgc cgaggatcac gatgtccgcc
     4681 gggtacgccg gaccctcccc ggtgagcacc tcgagtgacc cgtccgccgc ctcgcggaag
     4741 ccggacgccg acgcgtccag caagacctcg atgccgtgct tgcccaggtg gcgctccacg
     4801 tagcgcgcca tctcggggtc gagcggcccc atgatctggt tgccgcgttg cagcaacgtg
     4861 acctcgaagc cgaggcccac caggttctcg gccgtctcga tgccgatgaa gcctccgccg
     4921 acgacgacgg cgcgcaactc ccttctccgc gtctggaagc ccgtgtagga gtccatgccg
     4981 gccgacctgc cgcgccgctc atcgatccag gcccggatct gaccggcgtc cggcaccgtg
     5041 cgcacgccga agatcccggg aaggtcgatc cccggcaggg gcggtcggac aggagccgcg
     5101 ccgggcgaga tcacgagctt gtcgtagcgc tcggtggtca cctcgccggt cgtgcggtcc
     5161 ttgagctgca ccgtcctctc gcccgccgcg atcccgacca cttcgcagtt cgtccgggcg
     5221 tctacggcga acatgtcgcg aaacgtgtcc gtggtcgcga ccaggaggtt cgcctgctgt
     5281 tcgatcacgc ccccgatgta atacggcagc ccgcagttgg cgtacgacac gtagggtccg
     5341 cgctccacca tgaggatctc ggcgttctcg tcgagcctgc ggaggcgtgc cgcacacgag
     5401 gcgccgcctg cgactccgcc cacgatgatg accttcatcc tgcctccttc acgccggatg
     5461 tctccaccgg cgatacacgt ggtcttgggt atggcacgct caagctccgc cccgcctctc
     5521 aggcctcgat cagctatacc gccagggacg agtcacgagc atgtagacac cccagatgac
     5581 aagtccgatg atggcgatcc agatgaagaa cctgaggttc gcgaacagga tccctgcgag
     5641 caggaacgcg agcaggatcg agccgatcac catcagcacc accatgaggg tacgcattcc
     5701 gaacctccgt ttcgtccttt gggcggaagc agggcgaggg cgctgccctc accccgcccg
     5761 agctt
//
LOCUS       DASUMX010000104         2310 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_172103, whole genome shotgun sequence.
ACCESSION   DASUMX010000104 DASUMX010000000
VERSION     DASUMX010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172103"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            130..951
                     /locus_tag="VFZ86_05055"
     CDS             130..951
                     /locus_tag="VFZ86_05055"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5641694.1"
                     /translation="MPRVRSSGAIIDYEDEGLGDPALLLLPTWCSTRAAFEPVMDLLR
                     ARRRVLALDWRGHGASGGPAGDFGEEDLVRDALAVIADSGAQSVVPVALGHAGWIGID
                     LRRGLGSGVPGLVVVDWVVSEAPAALLTTLEDVRSDADRDDAVAGLLQQWQAGVPDPG
                     LAALLADMGAASDAMWDRAAREIESSYARFGSPLRAVAGLEHPPPVLHLVPAADDAFA
                     FERQRAYEAEHHWYHAASLPARSHFPLFEAPRNAADEIERFARRVGGRQTHRRAA"
     gene            1051..1692
                     /locus_tag="VFZ86_05060"
     CDS             1051..1692
                     /locus_tag="VFZ86_05060"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5641695.1"
                     /translation="MPPTARQDKRHQETRDEILAAARDLVAEKGARKLTVRELAGRVD
                     LTAPALYRYFPNGKDEVLLALAAQDLDHLSTHLLTAPKALPVDERLIEIGLAYIQFAR
                     EHPAELDLLFESLSGLSRETQNGSVELLTTVPLFTLVQDVLRQGIAQGVLHARSKHEE
                     EDAMLMWHGAWALVHGLVAVERTHQHHDELFRSRARDVLRAFVNGLKTDWTSS"
     gene            1742..>2310
                     /locus_tag="VFZ86_05065"
     CDS             1742..>2310
                     /locus_tag="VFZ86_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641696.1"
                     /translation="MRRMPPTERLARFSARRPWLTIAAWLVIVVAAGGLLVAFGDSLQ
                     AADDFIGRPESKRAEDLLAERLPGSDADTEIVVVRSDSFTVDDPAFATRVRELATAIR
                     ALAPRDVHTVATWYDAQAAADAAAAEARAAQAAPGTGAATVKAAEAAARQAAGQAAAL
                     VSADRRTTILPVTMAVPSGEVDEHMARLY"
BASE COUNT          331 a          836 c          813 g          330 t
ORIGIN      
        1 ccccgcggcc catccccctt tctggagccc tgagcgccac cctcagcagc ggccattgcg
       61 gcaacggact ggtcggccgt caatcgggaa cctgcctccc atgtgcatcg gctatccatg
      121 gaggctgtca tgcctcgggt gagatccagc ggcgcgatca tcgactacga ggacgagggg
      181 ctcggcgacc cggctctgct gctgttgccc acctggtgca gcacgagggc tgctttcgag
      241 cccgtgatgg acctgctgcg cgcacggcgg cgcgtgctcg cactggattg gcgcggtcac
      301 ggcgcctccg gcgggccggc gggcgacttc ggggaagagg atctcgtgcg cgacgccctt
      361 gccgtgatcg ccgacagcgg cgcgcagtcc gtcgtccctg tcgcgctggg tcatgccggc
      421 tggatcggca tcgacctgcg tcgcggcctc ggctcgggcg ttcccggtct cgtggtggtg
      481 gactgggtcg tgagcgaagc gccggcggcg ctcctgacga ctctggagga cgtgcgctcc
      541 gacgcggaca gggacgacgc ggtcgccggc ctcctgcaac agtggcaggc cggcgtcccg
      601 gatcccgggc tcgcggccct gctggccgac atgggcgccg cctcggacgc gatgtgggac
      661 cgggcggcgc gcgagatcga gagctcctac gcgcgcttcg ggagcccgct gagagcggtc
      721 gccggcctcg agcacccccc gccggtcctg cacctggtgc cggcggccga cgatgcgttc
      781 gcgttcgaac gtcagcgggc ctacgaggcg gagcaccact ggtaccacgc ggcgagtctc
      841 ccggcacgct cgcacttccc gctgttcgag gcgccgcgga acgcggcgga cgagatcgag
      901 cggttcgcgc ggcgcgtcgg aggaagacag acccaccgcc gcgccgcctg agcgaacgcc
      961 gccgccgaca tcgggcttga cggcatctca tgccggccct atagtgaacg aagttcacgt
     1021 aagggaagac tgttcactga ggtcgtcacc gtgccgccga cagcccgtca ggacaagcgc
     1081 caccaggaga ccagggacga gatcctcgct gccgcccgcg acctggtcgc ggagaagggc
     1141 gcgcgcaagt tgacggtgcg ggagctcgcc ggccgtgtgg acctcacggc tccggccctc
     1201 taccgctact tccccaacgg caaggacgag gtgctgctcg cgctcgccgc ccaggatctc
     1261 gatcatctgt cgacacacct cctcaccgcg cccaaggcgc tgcccgtgga cgagcggctc
     1321 atcgagatcg ggctcgccta catccagttc gcgcgcgagc acccagcgga actggacctg
     1381 ctcttcgaga gcctctccgg cctctcgcgc gaaacgcaga acggcagcgt cgaactgctg
     1441 acgaccgtgc cgctgttcac cctcgtccag gacgttctcc gccaagggat cgcccagggc
     1501 gtcctgcacg cccggagcaa gcacgaggag gaggatgcga tgctcatgtg gcacggcgcc
     1561 tgggctctgg tccacggcct cgtcgccgtc gagcgtacgc atcagcacca cgacgagctg
     1621 ttccggtcgc gcgcccgcga cgtgttgcgc gcgttcgtga acggcctcaa gacggactgg
     1681 acgagcagct aggctgcgag cggacggggc agcatggccc cgccatcccc gggagaccgc
     1741 gatgagacgc atgcccccca cagagagact cgcgcgcttc agcgcgcgtc ggccgtggct
     1801 gacgatcgct gcctggctgg tcatcgtggt ggctgcgggc ggcctgctgg tcgccttcgg
     1861 cgactctttg caggccgccg atgacttcat cggccgtccc gagtccaagc gggcggagga
     1921 tctgctcgcg gagcggctcc caggctccga cgccgacacc gagatcgtcg tcgtccgctc
     1981 ggactcattc acggtggacg atcctgcctt cgcgacgcgt gtgcgcgaac tggcaaccgc
     2041 catccgcgcg ctggcgccac gggacgtgca cacggtcgcg acctggtacg acgcacaggc
     2101 ggcggcggac gccgccgccg ccgaggcccg cgccgcgcag gccgcgccgg gcaccggcgc
     2161 cgcgaccgtc aaggccgccg aggcggccgc ccggcaggcg gccggtcagg cggcggccct
     2221 ggtctccgcc gaccgccgca cgaccatcct gccggtgacg atggcggtgc cgtcgggcga
     2281 agtggacgag cacatggctc gcctgtacga
//
LOCUS       DASUMX010000105        42744 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_747555, whole genome shotgun sequence.
ACCESSION   DASUMX010000105 DASUMX010000000
VERSION     DASUMX010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 42744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42744
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_747555"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..597)
                     /locus_tag="VFZ86_05070"
     CDS             complement(130..597)
                     /locus_tag="VFZ86_05070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HEX5641697.1"
                     /translation="MGNPILCDDGIGIRLARHIGSLLGATPGLVVEPECTVGGLNLLE
                     LVAGFDRLVVVDSIKTRGGRPGDWYRFDGAALRETMNLSNVHDANFATAMELGRRMGM
                     HVPGEADIHVFAVEVRENLAFGECLSPELEDALPLLCEQMLDEIEELLRERTH"
     gene            complement(665..2131)
                     /locus_tag="VFZ86_05075"
     CDS             complement(665..2131)
                     /locus_tag="VFZ86_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013684228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HEX5641698.1"
                     /translation="MSRRISIDPITRLEGHGKIEIFLDDAGAVEDCYFQIPELRGFER
                     FVVGRPIEELPRLVTRICGVCPASHHMASAKAVDGCFGGEVAPLAVKLRRMYYNAHYI
                     HSHIAHFYALAAPDFVLGPDADPAIRNVLGVVAKVGLEIGGAVLASRSKAQEIQRIIG
                     GRSTQDIWCIPGGVAKGLKPEELDQIRPMVADLLEFTQFSLQLFRDVVLANPTYLDII
                     ANGPYTLDVQNMGLVDEHDAPDFYDGQVRVVDFEGKELHRYDAKDYADYVAEHVEPWT
                     YLKFPYLKQRGWKGFKEGIDSSIYCATPLARLNVAERMATPRAQEAFEEMFSTIGGHP
                     SRLLLAQHWARLVEMVQNAETLQSYCDDPEITGDQFRVIPQRVTGEGVGVVEAMRGTL
                     THHYTCDANAICTSANLIVGTTNNNAPIQMVTRKVAETLIQPGTDPDQGILNMIEMAF
                     RAFDPCYSCATHSMPGQMPLRVRVRRGGEVTHEFRRGC"
     gene            complement(2181..3146)
                     /locus_tag="VFZ86_05080"
     CDS             complement(2181..3146)
                     /locus_tag="VFZ86_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEX5641699.1"
                     /translation="MATDKLQIALYWGAACGGCDVAVLDTDEFILDVAAAADIRLWPI
                     AVDGKYADVEAMEDGELDLTIYNGAVRNSENEHIAKLLRAKSKLLVAFGSCAHIGGIP
                     GLANLTSRDEILTTAYLENPSLERGSKTLPLPESGVNGSTLTIPSFYRRVYKLDDIVP
                     VDYYVPGCPPAFDQVKAVLQAVVAGELPAPGSVVGASERALCDDCPRVKSEKRIKHFY
                     RPWQIMADPDACLLEQGILCAGPVTRGGCGARCPSSSIPCRGCYGPLDGVVDMGAKLL
                     SAVVSVIDSTDPEEIERIIDGLPDFTGFAYRFSAPSSLLQRSVRQ"
     gene            complement(3150..3584)
                     /locus_tag="VFZ86_05085"
     CDS             complement(3150..3584)
                     /locus_tag="VFZ86_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HEX5641700.1"
                     /translation="MTFEPKIVGFLCNWCSYSGADLAGTSRIHYAPNVRVIRVMCSAR
                     IDPTFVIKALADGADGVLVCGCHPGDCHYSEGNYKTMRRMPLLKRMLADFGIEEERVR
                     LEWVSASEGMRFADIVDDMTERLRALGPSPVKMALAGDAVER"
     gene            complement(3581..5305)
                     /locus_tag="VFZ86_05090"
     CDS             complement(3581..5305)
                     /locus_tag="VFZ86_05090"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HEX5641701.1"
                     /translation="MPEPRTGVFICNCGTNIAKVVDCDAVAEAAAGMPGVVVARSYKY
                     MCSNPGQEMIAQEIGDHDLERVVVAACSPRMHEPTFRRAVETAGLNPYLVEMANIREQ
                     CSWVHDDGARATEKAEDLVRAAVMRVAYHEPLERLSVDMCPNTLVIGGGIAGLTAALE
                     LAGAGKLVYLVERQPGLGGNVARVDLTAPYLDSAPDILTDRITRVVEHPDITVMTNSE
                     LVRLDGFVGNFTATLAVNGNDGPGEERRVDVGSVIVCTGYREFDASRATHLGYGKHPN
                     VITSFEFEHMLRQGRIETKEGRLPQYVAIVHCVGSRSAEFHGYCSRVCCMTALKYAHE
                     VKSALPGSYVSDVYVDMHAFGKGCEDFYRRSAEVKTLFLMYGKGEHPVVRRADPGDDC
                     EMLITVDEQLSGETIELPADLIILMVGMEPREDAESVTRLVNISQDKDGWFIESHPKL
                     DPVATTTDGVYIAGACQSPKDIPDSVAQARAAAARILGKIAKGKLEIDAMFAEIDQDR
                     CSGCRVCNEICPYSAIEFDAAEHRSHVISALCKACGACVATCPSAAIKARHFTDEQIF
                     AEIAGVLG"
     gene            complement(5319..6485)
                     /locus_tag="VFZ86_05095"
     CDS             complement(5319..6485)
                     /locus_tag="VFZ86_05095"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX5641702.1"
                     /translation="MAIRANPKLIEELEPYGAEDVMKCYHCGNCSAVCPFSADPYVFP
                     RKPMRYLQMGLEEKLEGQLDPWLCYYCGECSDQCPRDAEPGETMMSLRRWLTSRYDWT
                     GISRLFYRSWRWELLAILLVAVITAVAFTLFGMSQGSLSDYDGPNAFLPSSAIHIFDW
                     VLAAVLLAFLLSNAARMWWFTMGRDKDLPKSPGSYISSAWMLPAHFLTQMRYSQCEHK
                     GPWVTHLALMLSYCVMLVLIMFFLAEMASGPSVDWRVHVLGYLATAGLVVSSILFLRN
                     RMKKSAAQYRHSHESDWIFLSLILAVALTGILQHILHRTGLDAAANVTYIVHLALVVP
                     MLALEVPFSKWSHLAYRPLAIYLAQVRATALARARRGDGKQQPSAEQAAPLRTA"
     gene            complement(6497..8308)
                     /locus_tag="VFZ86_05100"
     CDS             complement(6497..8308)
                     /locus_tag="VFZ86_05100"
                     /inference="COORDINATES: protein motif:HMM:NF024842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HEX5641703.1"
                     /translation="MRIGVYICRASAETGGGANLESVGHYAANLPQVEVVRSLGLLPR
                     LDPEALADEIVASDLDRIVIAGDSPGFFKPAFTRAMALALRAPEEVRLASFREHGVSG
                     DGATERAKAITACAVFGVPFTLAARPKDTKWTAAALVIGGGIAGIQAALEIADGGKKV
                     YLVERTGTIGGHMAMFDKTFPTLDCAACILTPKMVAVGQHEMIDLLSYSEVESISGVP
                     GSYRATVLRHARRVDEDACVACGICSDVCPVRVPSEFDAGIAERKAVYIPFPQAVPNA
                     YLVDEASCTYVQTNGQKCGACAKKCPKECIDLDARGDVLQLEVGSVIVATGYEVLDAS
                     SLDRYGYGVHPNVLTALEFERLTNASGPTGGHIVTKSLKHNKRKKVDEWVFDPEGASP
                     KSVAIIHCVGSRDTNHNAYCSRVCCMYSLKFAHLVKEKLPDAACYEFYIDMRAFGKGY
                     EAFFERIQDEGTFIVRGRSATVTERDGQMMVKGEDILSDSVVEFPVDMVLLAVGLVPA
                     DRSDELARMLGIPRDDYGWFTEADYNGDPTGTERGGVFVAGVCQGPKDIPDTVAQASA
                     VAARALEGAMTGRGLQSLASLSLSDIEARAKSLVPAS"
     gene            8744..9952
                     /locus_tag="VFZ86_05105"
     CDS             8744..9952
                     /locus_tag="VFZ86_05105"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEX5641704.1"
                     /translation="MINQKVAAKQSLNDLADFLFEGTRTLIPCDRIGLAFVDESGRRV
                     FAHYARADYQPLLLTKGYTGDLKGGSLEHVIRCGEPRIIDDLEAHAREHPSSHASGLL
                     VREGVRSSLTCPLVVEGRVVGFLFRDSTRPRAFDAHHVQLEMAISERLSQAVEKAWRI
                     EQLTAANHAYLEMLAFVSHEIKNPIASLVTDTRLLADGYLGPLEPQQVEKMERVIAKG
                     QYLLDLVQDYLDLARIDGGGLELVRCAGVDVVEEIVAPSIDLVRAQIDQAEMRLHLDL
                     PAGAVTAECDPALIRIVLVNLLGNAVKYGTPGGEIRLRVVREPDRLEVSVWNEGKGFL
                     PEDRGRLFQRFSRLRSSGGANKRGTGVGLYTSWRIMQLHNGRIRARSDPGQWAEFSIE
                     LAQPLDLPSG"
     gene            9985..10995
                     /locus_tag="VFZ86_05110"
     CDS             9985..10995
                     /locus_tag="VFZ86_05110"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5641705.1"
                     /translation="MSRPLLGRVVLVNALVVAAVLAAALILQTREGGIGPGPAAGLLA
                     VGLAVSILANVLVVGPAGGVLLEITRAMETMHKGDLGCQVEERRGGAGLRRLTASFNA
                     MCMRLGRESLSYAERQLGSVEEERRRIGRELHDETSQTLAATLVRLDLCDRAIQDHDA
                     TAAHAQVVDCKTLIAHTMEEIKLLVYDLRPVMLDDFGLVPTLRWYIQSHLQGAGPAIV
                     TDFDDAGLRVPGHIETALYRVTQEALANAVKHSDATKVHVRLVTKPGFVTLAVIDNGR
                     GFDGGAALYDSGHTGVGLLSIKERVELAGGTVNIESAKGRGTRLYVVIPLATEESVEE
                     MT"
     gene            10992..11672
                     /locus_tag="VFZ86_05115"
     CDS             10992..11672
                     /locus_tag="VFZ86_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012892500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5641706.1"
                     /translation="MNAAKTRDEIKVLLVDDHAILREGVHALLAREPDIAVVGEAADG
                     QEAVEQVPRLRPDVVIMDIVMPRMNGLEATRLIRERHPEVRVLILSMYDDQEYVVQII
                     QAGASGYVLKRVVTEDLVRAIHEVHAGESFLYPPIAATLIGDYLRVTRGEAVGHAGGG
                     EALTAREREVLKLIADGNSNQEIAEQLGLSRKTVDSHRANAMRKLDLHDVTEVVKYAL
                     RTGLITLD"
     gene            complement(11723..13078)
                     /locus_tag="VFZ86_05120"
     CDS             complement(11723..13078)
                     /locus_tag="VFZ86_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641707.1"
                     /translation="MARTCPACGRANDDDASFCQGCGSALAEGARETRASPPLPPPGG
                     RRAPAWLVVSVVAVVIVVIAAAALLLFPRGKKQVAGDRSPVPSAGATVAVSPSPALGR
                     YLAGAVGSRADRLAAIDEDGSVHPIAEFSGEQLREIVYSPDGKWLACVAGTYERSELW
                     LFDAANGDARQVTADAPNVLAVDSIAWLSATELLVAGYTEPPRTTGQNADLLVYDTTA
                     LGFSPLLDAGGVSLRGVAVSASREGAEVAFVSYTDVTTGRYGMKTGLEHLEVLDRASG
                     RVTELDAGKAFFDVDARAFDEPLISPSGEALIYRRAGSDVGTSYTVVGADGTILMPAK
                     EAQFPAGYAWDPDGTRVVFAGVPPKRVGRMTFWVFDTETGGRPVVLARHKGVFVQDLS
                     WSPDGETIAWAAYDEEKYRTGTIYLMPAGGGGSTAFAERALSPVWAPSAAPSLQTSPS
                     P"
     gene            complement(13235..14455)
                     /locus_tag="VFZ86_05125"
     CDS             complement(13235..14455)
                     /locus_tag="VFZ86_05125"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5641708.1"
                     /translation="MIRPSPRRWVAQLRTLDPNVRRLGLVSMLADLSSEIAYPLFPIF
                     VTGVLGAPVALLGLIEGIAEATASITKYPFGQAADYTGRRRPFVLGGYSLGALGKLVV
                     ALAAGWPLALAGRFTDRLGKGMRTASRDDLIAANTRPGQQGLAFGLHRTMDTMGAVLG
                     PLVALVLVQADVPLRWIFAVAVVPGVLSVVAIVFLVREHREAPQRAAFRLSLPTSPAF
                     RWLLAGSLLFAAGNSSDTFILLKAQDVGMSTSAVILFYVLYNVVYAAGSLPLGGLSDH
                     VGQYPVVIAGYLVFAAVYAGFAAAGSGWTLAVLFAAYGLYIAATEGTSKALIGRAIPT
                     GERASALGLYSTATGLATFAASTVGGLLWSAWGPWATFAYGAAAALVAAGLMFAGRGR
                     VRGALAPEKPAPAG"
     gene            complement(14522..14920)
                     /locus_tag="VFZ86_05130"
     CDS             complement(14522..14920)
                     /locus_tag="VFZ86_05130"
                     /inference="COORDINATES: protein motif:HMM:NF017690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5641709.1"
                     /translation="MGDLSDTRIRVIKPDEVTWDDAMLNPGEQDLPGKELTAAMSADD
                     KFSFGLWKRDVQRRHFERPYHEVAYIIEGEVEITDAEGDVHVAGPGDIVITPKGSVGE
                     WKNLSPVKKVWGIYEEADAGLTAYLGPGGF"
     gene            15074..16381
                     /locus_tag="VFZ86_05135"
     CDS             15074..16381
                     /locus_tag="VFZ86_05135"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX5641710.1"
                     /translation="MQLQSALEERVVQAVRDAKDELVGLAAELVALDTTARLPGDPAR
                     DEEKLQRLLAARLEALGAAVDLWEPEPTGDGDRFVPAGLDFRGRPQLAAVLPGSGGGR
                     SILLNGHIDAVDAEPRDEWLSDPFVLTERDGCLFGRGANDMKGGIAGLVIALETLRRQ
                     GVRLAGDVVCCTVTDEESSGAGGRAAVAHGVRADAGIAAEATDFDAWVSCRGTVTPTI
                     TVAGRAGHAEMPQPDWREGGAVNAIEKLVAVVDGMGALRAEWKARPDHVHPYLAPGDI
                     VPTIVHGGTWMVTIPASCAVTADIMYLPQHVDAEGTGRAVEAEVIDRLTRAVAGDPWF
                     AEHPLRFTWSDDVVPAEMPADHPLVTTALAGAATLGHRGEPAGLNSWHDAATFTRAGT
                     PTFSFGPDGFDTAHAVDERVSVGGLVDFSAAVALTLLRWCGAS"
     gene            16378..16659
                     /locus_tag="VFZ86_05140"
     CDS             16378..16659
                     /locus_tag="VFZ86_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEX5641711.1"
                     /translation="MIVHLSIHTPRPGRERDLIDSMHRYGAAGAGLPGFIDAKTLRDR
                     HTGRLVGMARWEDEASWRAGVEAMRAAVADDPFDEWEAAEVEGFFLEEV"
     gene            16724..17641
                     /locus_tag="VFZ86_05145"
     CDS             16724..17641
                     /locus_tag="VFZ86_05145"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX5641712.1"
                     /translation="MTPAPTRDRRRALVADAALLSVAIVWGLNFVVIKDAIADTGPMT
                     YLLWRHILAAVMLAAVMPRTVRQTGRRDWLYGAVLGLFLFIAFVTQTIGLQWTTPGKS
                     GFITSLYIVMVPFLYWIVARRSPGWTQIGGAVIATVGLGFLSFRGGIGMSTGDLLTLA
                     SALAFAAQIVATGFFAPRTTPAVLALTQIVAAAVLFVVVTPFVEHITWDFGRQAWAAI
                     VWTALTGTVFGFLVQAWAQKSTTSTRAAVILGLEGLFAAVFGLLFGMDVLSWRFAAGA
                     ALILAGVIVIETLPGRGAPNPADVLPRAD"
     gene            17731..18312
                     /locus_tag="VFZ86_05150"
     CDS             17731..18312
                     /locus_tag="VFZ86_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641713.1"
                     /translation="MDWDWEDRPPKDPPQPRRAPDGPDDERDLAQPEIPSEAAAPDPS
                     PGPEPQEPEPERPEDASPFQELPPLDPAPDYVLPGRPGSPTVVESPGAESGRPPTGTI
                     EAAERAVVGAERRDRDQYRPHSAEDRAAARAQRRRKLRQRRLIALAVALAIVVLLVVL
                     VVRACGGPDAAAAVAAFAVPFVSRRLLVATERP"
     gene            18309..19367
                     /locus_tag="VFZ86_05155"
     CDS             18309..19367
                     /locus_tag="VFZ86_05155"
                     /inference="COORDINATES: protein motif:HMM:NF026220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HEX5641714.1"
                     /translation="MRPGRVAARALVAVIASLALLAGCGGAGPETADRPFGDPPPAVP
                     GIHAWAAGEAGVLLVTADGGASWMRREFFLPQRGVDVAFADAQTGWLVTDAGTVLATT
                     DGGTGWTVVEQAELTAKAIAATDSRRAWIVGSPSGAADEPGASAVLRTTDGGEVWRRT
                     GFGTAQLADVAFADERRGVLVALDRIWSTRDGGRTWRLRRELPMTVLTSVAAGDPRRF
                     WVAGWGTQLGDPLVFATRDGGATWRRLRVDVAPANPGELQARQIACAGESLWITCAAG
                     VLASADGGRTWELQQVPAGQPLAIAAADEEHVLATTGSQPILASADGGATWRAFGSEG
                     FLEQPLVAIDAVVAQPAQ"
     gene            19412..20368
                     /locus_tag="VFZ86_05160"
     CDS             19412..20368
                     /locus_tag="VFZ86_05160"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX5641715.1"
                     /translation="MTTPTGSTLVSRRRALVADVLLFSVAIFWGSNFVFIKDVVERTT
                     ELAAGSIVAGTMLYLVLRYVVATGIFAAVQPRSWLDSSRGDWARGGLLGAFYLTALIL
                     QTIGLQRTSPGVSGFITGMSVAMVPFLYWFVARRSPGRWQIIGAIVATVGLGALSLQG
                     DFSIRWGDAITLLGTLFYALHIMTTGFFAPKVRPTTLAVTQMAVSTLVLVVVTPFFVR
                     ITLDLPWQVWAAVVWTAVSGTIYAFFIQSWAQRYTTSTHAAILLGFESVFAAVAGIIA
                     GMDSVTWRLLVGGSMMLTGVFIVELLPGSKAIVEEIEAEEAT"
     gene            complement(20361..20753)
                     /locus_tag="VFZ86_05165"
     CDS             complement(20361..20753)
                     /locus_tag="VFZ86_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641716.1"
                     /translation="MSRKVVLIPILAVVVVAVAVITAAVWRQADAGPPIQTKALGVWQ
                     EQTTSQPIRLTVSAADAPADVARYWVTYAPTSAEPLPARLEGDRILVWGEGTQEVRWV
                     ITYDEGADALLLASPSGSERHILRRVST"
     gene            complement(20787..22628)
                     /locus_tag="VFZ86_05170"
     CDS             complement(20787..22628)
                     /locus_tag="VFZ86_05170"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5641717.1"
                     /translation="MAARTRQAGPLIKVSMVLILLLVLAAAGGVARAAASSSPSPGEK
                     VTLHIGMVQEPDNLNPFIGIQGIDYMFWHMNYDFLVGFDSKTLEPRPELATEWEVSED
                     GKEWTFTIREGSTWQDGQPVTARDVAFTFNYIVDNELLNLSTYTNGITGAEAIDDTHV
                     KVMTAAPKANMLRMVVPILPEHIWSKVSGKEATTSYQNKPPIIGNGPFQLVEWQKGKF
                     VRFEASPTYWAGRPKVDEVVFQLYTNPDTMAQDLKLGTIDGAVDIPPAQFKPLGDEEG
                     ITTSQSTSWNFIELAMNCYDSPNSKGNPVLKDQQFRTAVNWAVDRQKVVDVAYQQYAT
                     LGSTLIVPYSKYHWEVPADQAFTYDPARANEILDAAGYEDVDGDGFRETKDGKKLSLR
                     FYATTDSVQNQTAAKLIVGWLEDVGIKLELQVIDAGTLINYQYEYTGDTYTPNWDLFI
                     WYWVQDVDPNFIVDIYTPQQIEGWNDCLWTDPEYTRLNDEQITTIDEAQRVPIVQQMQ
                     QIFYEGAGYAILAYPYQLEAFNTDEWQGWVHVPSEASGQEGAVIYSYNNIDTYRFVEP
                     KVAAAATAEDEPIVNWTWLAVGLGGVLILLVVMFVLTRLFGRRREEE"
     gene            22826..24049
                     /locus_tag="VFZ86_05175"
     CDS             22826..24049
                     /locus_tag="VFZ86_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00300 family protein"
                     /protein_id="HEX5641718.1"
                     /translation="MPDTPYEDVQIEGHLIDSLMMPHIMDEVMDLDGEFEMLAFEVGR
                     MKDDPSHAVMRIFGRDAAHLEEILTAITEFGVVAVKPQDVVLEEADIDGVFPDAFYST
                     TNLTTFVRVGGEWIRVEHPEMDCGIRVDVEAGAAETVAMSDVKAGDRFVTGHRGIRVL
                     PLERPRESQPFEFMASAVSSEKPKAQIVHEVAHILRQVKEEGRLTIAVVGPAVVHTGA
                     APQLARLIEAGYVGCVFGGNAVATHDIESNLYGTSLGINLKSGAPVPGGHEHHLRAIN
                     TIRRAGSIQAAVDGGVLTGGLMYTLTKTGTPCVLAGSIRDDGPMPEVITDVMQAQKEM
                     RRYAQQAGACLMLSTMLHSIAVGNMLPASVRTVCADINPAVVTKLADRGSFQAIGIVT
                     DVGLFVEQLADELTR"
     gene            complement(24082..25863)
                     /locus_tag="VFZ86_05180"
     CDS             complement(24082..25863)
                     /locus_tag="VFZ86_05180"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5641719.1"
                     /translation="MGRFLIIVACAALLLVMPSGASAGASSSPAPAATVLRLGTTQDL
                     DSLNPFAGYTSVAYEVYHLNYDMLTGSAPDGDVRPEIADSWAMSEDGLTWTFRIHPGI
                     RWQDGVPLTARDVAFTFNYIIDNELSAFTGSTTHIKKAVAVDDTTVEFRLERPKSNML
                     RLPIPILPEHIWAKVPGDEAATFAPEPPVIGSGPFQVVEVKKGSYVRLVANTDYWKGA
                     PKIDEAIIEIYQNQDTMAMDLRSGTIDVAEDLPAAQFSALQNAPGITAKAADIRYFVE
                     VAMNVYQDEDSLANPVLRDVAFRRAISWAVDKHAIVETCLGGYGAVGQSIIVPGTDGA
                     WAPSAEEAFGFDLTKAGAMLDAAGYRDADGDGIREDKQGAPIELRLWTRSESPEQQRA
                     GKLIAGWLGQLGLKIQLSVENDGALVDGVYHYEGEDYAPDYDLFIWGWGSIADPGYQL
                     GFFTSSQIEGWNDCCWSNAEYDGLFVQQDAEMDWPKRELQVQRMQQIFYADAPYMVLY
                     YPKALIAYNTAKWEGWVPYPGDNGMVVFSNDNIDSYVQVHPRAASETAAETEGPDTAL
                     IAGVAAAALAVAAIVLLLRRRGRAVEQ"
     gene            26292..27860
                     /locus_tag="VFZ86_05185"
     CDS             26292..27860
                     /locus_tag="VFZ86_05185"
                     /inference="COORDINATES: protein motif:HMM:TIGR03975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RiPP maturation radical SAM C-methyltransferase"
                     /protein_id="HEX5641720.1"
                     /translation="MLLIEAPFEPLQRGRLAPALLKAELQQRGLRCDVEHLGHAFAAL
                     LGRSEYARLIREVPPGALALDWVFAEALFGTGVPAPRAYVADVLAGHWRLSGELVELV
                     LRARGLVAGFLTRALLEIDWSRYTVLGFSALVGQAVSSLALAKAVKEAHPGVHVVFGG
                     PAWHGVMGRRQLAEFLFVDAACEGDGDLVFPAYCLWLAAGCRGPWPAGLLLRSSGGSG
                     GQPPVSPSMSDLDALPTPDYTDFYRALALWGGDAGVAQLAAEASRGCWWAARRPCAFC
                     GLNRPTRRYRPKSADRILIELRELDRRWQPWLIDLVDNVVPPAFLREVLPRLAASPLT
                     ARLFFEARPELTRAQLRLAARAGAHVQLGIESLSQHVLDLLGKGTDARLNVRELKWCR
                     DEGVPVTWNLLHGIPGETARDYHQMRSLLPLLRPLPPPEGCGAVEVHRFSRYFSQPAA
                     FGIVNVRPASAYSYVYPFNGAKLRDLAYLFDHELVQPVRGDRQLQRELSAFLLQAHEW
                     IASATAPRQAAPPV"
     gene            complement(27839..31225)
                     /locus_tag="VFZ86_05190"
     CDS             complement(27839..31225)
                     /locus_tag="VFZ86_05190"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5641721.1"
                     /translation="MDGVCQSCSEPLPDGARFCPSCGARRIAPAARTERRLITTLFCD
                     LVEFTAMAERSDPEDVDALLRPFYALARGVIEQHGGVVEKFVGDAVVGVFGVPRAHED
                     DPERAVRAAVRLQLRLGELPHHQGEPVLARIGVNTGHALVQLDVDPLAGESFQAGDAV
                     NTAARLLALAPPGGIVVGETTHAMTAHVTEFTALPAVRLRGRQALERPWLVTGTVAQL
                     GVQLQRPEAAPFVGREVELGILTGLFEKSMRSASPQAVLLVGEAGIGKTRLLVELARR
                     LDARPDLLAQWRQVRCSPFEEGAGLWPLAQVVREHAGVVAGDDDAAVQTKLERCFAEA
                     RDEPWFLQRLRALLGSGGDRASSSENFAAWSRFIELMARVRPTVLVVEDIQWASSTLA
                     EFLQHLRSTIDDVPLLTILTARPELLDGPGDRRTAVAGDPPPPMTLALHPLSERETER
                     LVSLLLHDGDADVSATVVGSCGGNPLYAEEMAGFLADRATGAGPKREMRPDLPPPSLQ
                     ALIAARLETLDAVERTVLSNAAVVGTTFWRGALHALDSSGAAAVDEALTRLTERELVR
                     PMAGSPATAAEFAFWHTVVRDVTYAMLPRLARASRHKGVADWLEHQEGQWSPAALIAH
                     HLTTALEAARHADAALAAELLEPAVEAHIRAALQTQPVDMRGAERHLDAAAALMPTGH
                     PRRPWVTIERGDALSHIGRLDEARACIEEGVRGAEEAGDRPVAAHGRTRLTRLMNWMG
                     DPDAPEVSSATLEMLRQDGPSATLVTALEEHAMTCVFAFRHSDAITAADAALSMAASL
                     DTPMPARALLWRGTARCGQGDAGGLTDLATALDQAIGQGRSRDLTSLYYNYAGALMTY
                     EGTEQSIAMCQAGVDDMVRRGDANGANALQAGMCFDLLCAGRWDEVLVAGPSLETALE
                     RAGDLQMLGEMRSMMALLALLRGDIEPAREKARQAEGTIASVAFGNHAWTCSCFLSAV
                     HHGLSDDEGALAELTTLEQATRGKRGAIATGLPFALRTLTALDASALAERLVDGLSHR
                     PFDIAVLSLWRALAAEHGGRWHEAVDAYRDAVVRWAALAFVTEKAQSQLGLGRCLSRI
                     ERSAEAAEPLAAAHAVFAELPMPAAMAEAAGLMTQTGGAA"
     gene            31433..32272
                     /locus_tag="VFZ86_05195"
     CDS             31433..32272
                     /locus_tag="VFZ86_05195"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEX5641722.1"
                     /translation="MRNKLPLLLVVVLIAACAVAAAPARAADSPAAIAREILGHAPRG
                     LAQTVVERGYVRVANDANYRPFSSVDTDSAGLVGFDVDYARAVGEILGLEVRFKNPAW
                     SWVPRGLVSARYFEVSIGGLKNTWKNRRTLAFTRAYYHLAAKVLIRRGGPAVTGVESL
                     AGRRVAAQSGTYLRFLERKTGARAVQYLHWQDARAALLRRDVPMWLLSAVTAGRLARE
                     DDRLQVIGPALFWDRCAVAVRRGEDDLVELLDFAVRRLKDDGTTQALIVKWFGAGDPG
                     IRP"
     gene            32295..32864
                     /locus_tag="VFZ86_05200"
     CDS             32295..32864
                     /locus_tag="VFZ86_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641723.1"
                     /translation="MTHERLEEQPRRPRWPWLVLILVVAAAVGAVSWRWVPPGDATMS
                     QVRAAEVAYESVFAPVAPEGAKPGAPLSVGQRAALEGELQARLEACCTRDFLRTQGRY
                     ARSMAKRVSTALANGTRYDPVHPYEHLRDLQFRRRLWNGDLVFDVYAPGETKVGGLPV
                     PETGFIKETIRFTKVDGVWKIAEAQHFGA"
     gene            complement(32871..33293)
                     /locus_tag="VFZ86_05205"
     CDS             complement(32871..33293)
                     /locus_tag="VFZ86_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005985189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5641724.1"
                     /translation="MEARISVITLGVADVPRARTFYLALGWPLSGEPEEQVAFFRNAG
                     SRLALCRLESIAEEAGQTPGEPGSIRATLAMNVESRELVDDCLDAAVGAGGTLLRPAQ
                     DRFWGGYSGYFADPDGHAWEVAFNPLWPIDDDGLPVLP"
     gene            33444..34655
                     /locus_tag="VFZ86_05210"
     CDS             33444..34655
                     /locus_tag="VFZ86_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HEX5641725.1"
                     /translation="MKPRIIRENVSLIGAVDWDRRLFDALIPLPDGTSYNAYFIAGSE
                     KNALLDSVDAPKWPVLSAQLDQLPTPDYLVIHHVEQDHSGSTPMLLERFPELTIVCNE
                     KAKGMLIDHLHVDDARFHVVADGDELSLGDKTLKFLFTPWVHWPETMSTWLEEGGVLF
                     SCDFFGSHYATSDVFADKHEVYDGAKRYYAEIMMPFGAQVLKNVEKVTQYPIEIIAPS
                     HGPVYDDPAFIIEAYRDWAGGEPQNIVCLPYVTMHDSTRLMVDHLTAALADRGIGVER
                     FDLTTLDAGELASSLVHAGTIVIGTPTVLTGPHPLAMYAAALANALRPRAKFATVIGS
                     YGWAGKAVETLVGMMPKLKLELLEPVLAKGMPREADYAALDALADTIAEKHQDLAPRN
                     FADLSVCRTDG"
     gene            34737..35606
                     /locus_tag="VFZ86_05215"
     CDS             34737..35606
                     /locus_tag="VFZ86_05215"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX5641726.1"
                     /translation="MLTVAFGLASALGYAVHDYLLVRIVRSVSVWTALMWVMLVGLAV
                     LLPLALVVDGLPSGDEQWRAAGFAAASGVLEFLGLGALLKGLSTGNLSVVTPLGSLAG
                     GFAAGAAILLGEALPPIAWVGLPLAVAGGLMASVERAPEAALGARRRTQATAGAGWAL
                     LSAALFAGTFLFFAEATALPPFSLAAIGRAGTVVIVVPVALLTGGLVLPREFRRRATV
                     AGVVDAGAFVALAAAISLGPVAIASVSVAQAGTWAVIIGLVVLRERLGRVQLVGVALT
                     CIAITMLAAAGLE"
     gene            complement(35642..36439)
                     /locus_tag="VFZ86_05220"
     CDS             complement(35642..36439)
                     /locus_tag="VFZ86_05220"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5641727.1"
                     /translation="MDGAPQLPVAADWFSLTWVTGRTAALTEPYVDDLLRASLWYFRG
                     RDRDLLIDTDNGVAPLRRHLARLARGGRPREIVCVVTHAHIDHIGGFHEFERRLLHPA
                     EQELATTPHNDAPLAPALWSEEILAQLAESGFVPPPVLVDALPSPGFDPFTFRPERAA
                     PTHFVRGGDEIDLGDRRFTIVDLPGHTPGSIGLIDHEERALVSGDAVYDGGLIDTLPE
                     SDVETYLATMEVLRRIDVDVVYPGHGRPFGRTTLRELAEAYLRERGG"
     gene            36569..37396
                     /locus_tag="VFZ86_05225"
     CDS             36569..37396
                     /locus_tag="VFZ86_05225"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX5641728.1"
                     /translation="MLTVLTGLLTALSYATSDMFSQRVTRQTRALTQMLWVLATGVII
                     ILPVALLIEGIPAAGEWGGAGLAALAGALYFFALFCLLRGLNVGDLGLVSALVSLQGA
                     YVAVAVVLLGTPVTLPLAVALGLCVFGAILTSIEGRAGSTKGAPWAIVAGILFACIML
                     CYNYADIGWLSQTAISRTVSLAVTLPVALLSGSVAVPKALRTRAVGAGVLELAGLSLL
                     TITFALGPPTVAGVTTTQFGTFAVILGFVLLHERPKPNQWVGIACTIAGVSLIAAIV"
     gene            37518..38882
                     /locus_tag="VFZ86_05230"
     CDS             37518..38882
                     /locus_tag="VFZ86_05230"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: protein motif:HMM:NF000818.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEX5641729.1"
                     /translation="MAANIIDDSHLKKTQEMFAKCQEVVSGGESSYARLIGTRPIVME
                     RGEGAYFYDVDGNAYVDWCIGYGPMIFGHRPKPILDAIIAQISQGGMLYTFPHELDYE
                     VGRKIVQAVPGIDQVRFANSGSEATQAAIRLARAYTGKDKIMKWEGSYNGFLDCHAFS
                     HVPALEAAGPEKFPRTLPSQAGIPKAVEGTVVIAPFNDIDAVEVLIKRNRDELACVLA
                     EPILGDAGIIPPVPGFLEGLRRICDENDVVLIFDEIITGFRVSLGGAQELYGVIPDIT
                     CIAKAVGGGTPGAAAFGGKKEFMDLEAEGVVLHGGTYSGNPMTLAGMNAALDILINDR
                     ERVYGHLNTIANAMVGGMRSIFAENGVPAYISQVGPMWQVFFGHEEPVTKYRQARASD
                     TRFFGMLQTECQMRGVYFHNFNFERFFASTAHTKTEVDRSLEVIAEATKIVKKRLGDL
                     PAGV"
     gene            complement(38902..39309)
                     /locus_tag="VFZ86_05235"
     CDS             complement(38902..39309)
                     /locus_tag="VFZ86_05235"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX5641730.1"
                     /translation="MSVFVDTSALVALLDAEDAGHGLMRPAWDHGVDDDAGFVTTNYV
                     LLEAITLVQARLGLEAVRLLTGEMLPMIEPLWVTDAEHGAGVGALLTAGRRRLSLVDC
                     VSFAVMRARQIDEYLGLDPHFDEQGFRRYAPAD"
     gene            complement(39306..39557)
                     /locus_tag="VFZ86_05240"
     CDS             complement(39306..39557)
                     /locus_tag="VFZ86_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015717122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEX5641731.1"
                     /translation="MVRMQIQFTEDEARRLRDEAAQRQVSISSVVREAVDERLIQRKS
                     AVSLEERKRRAKAACGRFRSGLGDVAARHDDYFVDSIEW"
     gene            complement(39653..40006)
                     /locus_tag="VFZ86_05245"
     CDS             complement(39653..40006)
                     /locus_tag="VFZ86_05245"
                     /inference="COORDINATES: protein motif:HMM:NF039183.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5658 family protein"
                     /protein_id="HEX5641732.1"
                     /translation="MTPAFRVRRTLPRPSESRDRLLLLLLAELWLLNAADLALTRYSM
                     WLGFATESNGIMDFFLRAGAVQALAFKLGIVTVGALLLWRLRAYRIATVAAGLLAGVL
                     AAVVVYQTLWVLHLR"
     gene            complement(40003..41133)
                     /locus_tag="VFZ86_05250"
     CDS             complement(40003..41133)
                     /locus_tag="VFZ86_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641733.1"
                     /translation="MPDDLTGRPAATPASSAAAPPVLVGATAAPGAGERLPRRPSWLR
                     ALVLSLVVLAAGSALGAAWYRFERSASIQPPAVERTAGGWRVTAHHQLAFSGLALSGD
                     RLLWQNGASIEYLDLASGDLRLLGPGPGMRTTWDPAVGERYAVWFEAEREASLAVQAV
                     AYDTESGRRWTLAEVGSVRSYPAISGDVAVWCSAPTIGAPTINGARVGSGEAFEVAQG
                     DGAPVVSGSLVVWATGWTGPFVAGDLVTGTTWPVSASLTGARLTAIALAGRTLVWGQG
                     EADGSGVVAAVAVDGGPTTTVASGVSGLAGPSYDGRTVVWAEQTASGDRVMGRRPGET
                     GAFVVAEADGAVTEVAVSGATVAWIQRMGGSTHAVVVTRLPQ"
     gene            complement(41118..42296)
                     /locus_tag="VFZ86_05255"
     CDS             complement(41118..42296)
                     /locus_tag="VFZ86_05255"
                     /inference="COORDINATES: protein motif:HMM:NF012945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641734.1"
                     /translation="MSAGSAAPPGAPRDVRISLSGFEAEPARGTPRTLKLCLLGFGSV
                     ARALCELLAAQERVLAGRHGLRVLISAVGTRRGSLLDPDGMTPAEALAAIGDGPAPPQ
                     APRPAPGLLAASEADVLVELTVMGDLSGVGAGAATYRPAATDHVLEAFRLGMDVVTAN
                     KGPIAWSWPEVSDAAAASGRRIRFESTVMDGLPVFSLLEYALPDCTVLGFEAVFNGTT
                     NFIIDAMGAGRTFDDALAQTQAEGYAEADPSNDIDGWDAASKAAALANVAMHACLTPA
                     DVERESLRDVPLERIVVARRAGRRLRLVCSVWREGEAGEASPAGPDAPHGSGPVRARL
                     RAEELDIEHPLAAVSGESLGALLHTDLMADVLVSERHALVPQTAYGVYADLLHLCRTI
                     "
     gene            complement(42293..>42744)
                     /locus_tag="VFZ86_05260"
     CDS             complement(42293..>42744)
                     /locus_tag="VFZ86_05260"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX5641735.1"
                     /translation="AAIDFIADLGERHVAMVEDRLPAGLQGRRRAVVAGMLAGEAYEQ
                     PLAKRLREQLAGIPGLTLYGPPEGSPRTSTVSFTVDGFQAGEVCRALGARGLFTWDGD
                     FYAHRLVELLGLHPRGGLVRVGLAPYTTEGELERLVAAVGDLSRSVR"
BASE COUNT         6134 a        14966 c        15122 g         6522 t
ORIGIN      
        1 cgcccggcgc cgggcgcgtt gtgtcgcccc ggtccggccg tggtcaccgg cgcagttcgg
       61 gctcgcttcg ggatgcaagg tccgcaggtg gacccgtaca gcagatctcg ccgcagcggc
      121 gcgagatctt cagtgcgtcc gctcgcgcag gagctcctcg atctcgtcca gcatctgctc
      181 gcacaggagg ggcagggcat cttcgagctc gggactcagg cactcgccga aagcgaggtt
      241 ctcccggacc tccaccgcga agacgtggat gtcggcctca ccggggacgt gcatccccat
      301 acggcgtccc agctccatcg ccgtcgcgaa gttggcgtcg tggacgttgc tgaggttcat
      361 cgtctcgcgc agggccgccc cgtcgaagcg gtaccagtcg cccgggcgcc cgccccgtgt
      421 cttgatggag tcgacgacga cgagccggtc gaaccctgcc acgagttcca gcaggttcag
      481 gccgcccacg gtgcactccg gctccacgac caggccgggc gtggcgccca gcagggagcc
      541 gatgtgtcgc gccaggcgga tcccgatccc gtcgtcgcac aggatcgggt tgcccattcc
      601 cagcagcagg gtccgcggcg gcgccggtcg tgtggggagc ggcgccggcc ccggaccggc
      661 gcggtcagca gccgcggcgg aactcatgcg tcacctcgcc gccgcggcgc acgcgcaccc
      721 ggagcggcat ctgccccggc atgctgtgcg tcgcgcagct gtagcagggg tcgaaggcgc
      781 ggaacgccat ctcgatcatg ttgaggatgc cctggtcggg atcggtcccg ggctggatga
      841 gtgtctcggc gacctttctg gtgaccatct ggatgggcgc gttgttgttg gtcgtgccga
      901 cgatgaggtt ggcgctggtg cagatggcgt tcgcgtcgca ggtgtagtga tgagtcagcg
      961 tgcccctcat cgcctcgacg acgccgactc cctcgccggt cacgcgctgc gggatgacgc
     1021 ggaactggtc accggtgatc tccgggtcgt cacagtacga ctggagcgtc tcggcgttct
     1081 gcaccatctc gacgaggcga gcccagtgct gcgccagcag caggcggctc gggtgtccgc
     1141 cgatggtcga gaacatctct tcgaacgcct cctgcgcgcg gggcgtcgcc atgcgctccg
     1201 ccacgttgag ccgggcgagc ggggtggcgc agtagatcga cgagtcgatc ccctccttga
     1261 agcccttcca gccgcgctgc ttgaggtacg ggaacttgag gtacgtccac ggctccacgt
     1321 gctcggcgac gtagtcggcg tagtctttgg cgtcgtagcg gtgcagctcc ttgccctcga
     1381 agtcgaccac gcggacctgc ccgtcgtaga agtccggggc gtcgtgctcg tcgacgagcc
     1441 ccatgttctg cacgtcgagc gtgtacgggc cgttggcgat gatgtccagg tacgtggggt
     1501 tggcgagcac gacgtcgcgg aagagctgca gcgagaactg cgtgaactcc agaaggtccg
     1561 cgaccatcgg ccggatctgg tccagttcct ccggcttgag acccttggcg acgccgccgg
     1621 ggatgcacca gatgtcctgc gtggagcgtc cgccgatgat gcgctggatc tcctgcgcct
     1681 tggaccgcga ggcgaggacc gcgccgccga tctcgagccc gaccttggcg acgacgccga
     1741 gcacgttacg gatggcgggg tcggcgtcgg gtcccagcac gaagtccggc gccgcgagtg
     1801 cgtagaagtg agcgatgtga ctgtggatgt agtgcgcgtt gtagtacatg cgccgcagct
     1861 tcaccgccag cggcgccacc tcgccgccga agcagccgtc gacggccttg gcgctggcca
     1921 tgtggtggct ggcggggcac acgccgcaga tgcgcgtgac cagccgtggc agctcctcga
     1981 tcggccggcc gaccacgaac cgctcgaacc cccgcagctc cgggatctgg aagtagcagt
     2041 cctccacggc gccggcgtcg tccaggaaga tctcgatctt gccgtgaccc tccagccgcg
     2101 tgatggggtc gatgctgatc ctcctgctca tgacgcgctc ccttccggcg tacatgagcg
     2161 gcgcgccgca ggtacgccgc tcattgacgc acgctcctct gcagcaggga cgagggcgca
     2221 ctgaagcggt aggcgaagcc cgtgaagtcc ggcagcccgt cgatgatgcg ctcgatctcc
     2281 tccgggtcgg tgctgtcgat caccgagacc acggcgctca gaagcttggc gcccatgtcg
     2341 acgacgccgt cgagtggtcc gtagcagccg cggcagggga tactgctcga cggacagcgg
     2401 gcgccgcagc cgccccgtgt caccggcccc gcgcagagga tgccctgctc gagcaggcag
     2461 gcgtccgggt cggccatgat ctgccacggc cggtagaagt gcttgatgcg cttctcgctc
     2521 ttgacccggg gacagtcgtc gcagagcgcc cgttccgacg ccccgaccac cgagcccggc
     2581 gccggcagct ccccggccac cacggcctgc agcacggcct tgacctggtc gaaggccggc
     2641 gggcagccgg gaacgtagta gtcgaccggc acgatgtcgt cgagcttgta gacccgcctg
     2701 tagaagctgg ggatcgtgag cgtggagccg ttgacgccgc tctcgggcag cggcagcgtc
     2761 ttgctgcccc tctcgaggct cgggttctcg agatacgccg tcgtcaggat ctcgtcgcgg
     2821 ctggtgaggt tcgcgagtcc ggggatgccc ccgatgtgcg cacacgaccc gaaggcgacg
     2881 agcagcttgc tcttggcgcg cagcagcttg gcgatgtgct cgttctcgga gttgcgcacc
     2941 gcgccgttgt agatggtgag gtcgagctcg ccatcctcca tcgcctcgac gtcggcgtac
     3001 ttgccgtcca cggcgatcgg ccacaggcgg atgtccgcgg cggccgcgac gtcgaggatg
     3061 aactcgtcgg tgtcgaggac ggcgacgtcg cagccgccgc aggcggcgcc ccagtacagg
     3121 gcgatctgca gtttgtcagt ggccacggat cacctctcga ctgcgtcgcc ggcgagcgcc
     3181 atcttcaccg ggctggggcc cagcgcccgc aggcgctcgg tcatgtcgtc gacgatgtcg
     3241 gcgaagcgca tgccttcgct ggcgctcacc cactcgaggc gcacgcgctc ctcctcgatg
     3301 ccgaagtccg cgagcatgcg cttgagcagc ggcatgcggc gcatcgtctt gtagttgccc
     3361 tccgagtagt ggcaatcgcc gggatggcag ccgcacacga gcacgccgtc cgccccgtcg
     3421 gcgagtgcct tgatgacgaa ggtcgggtcg atgcgcgccg aacacatgac gcggatcacg
     3481 cgcacgttcg gcgcgtagtg gatgcgactg gtgccggcga ggtcggcgcc ggagtaggag
     3541 caccagttgc agagaaagcc gacgatcttc ggttcgaacg tcatcccagc acccccgcaa
     3601 tctcggcgaa gatctgctcg tcggtgaagt gcctcgcctt gatcgccgcc gacgggcagg
     3661 tggccacaca cgcgccgcag gccttgcaca gtgcgctgat cacgtggctg cggtgctcgg
     3721 ccgcatcgaa ctcgatggcc gagtacggac agatctcgtt gcagacgcga catccggagc
     3781 agcggtcctg atcgatctcg gcgaacatcg cgtcgatctc gagcttgccc ttggcgatct
     3841 tgcccaggat ccgggcggcg gcggcgcgcg cctgcgccac cgagtcgggg atgtccttgg
     3901 gcgactgaca cgcgccggcg atgtacacgc cgtccgtcgt ggtcgccacc ggatccagct
     3961 tcgggtggct ctcgatgaac cacccgtcct tgtcctggct gatgttgacc agccgcgtga
     4021 cggactcggc gtcctcgcgc ggctccatgc ccaccatgag gatgatcagg tcggcgggca
     4081 gttcgatggt ctctcccgac aactgctcgt cgaccgtgat cagcatctcg cagtcgtcgc
     4141 ccgggtcggc ccgccggacc accgggtgct cgcccttgcc gtacatgagg aacagggtct
     4201 tgacctcggc gctgcggcgg tagaagtcct cgcacccctt gccgaaggcg tgcatgtcca
     4261 cgtacacgtc ggagacgtag ctgccgggca gtgccgattt cacctcgtgc gcgtacttga
     4321 gcgcggtcat gcagcagacg cgggagcagt agccgtggaa ctccgcgctg cggctgccga
     4381 cgcagtggac gatggccacg tactggggca ggcgtccctc cttggtctcg atgcggccct
     4441 gccgcagcat gtgctcgaac tcgaacgagg tgatgacgtt ggggtgtttg ccgtagccga
     4501 ggtgcgtcgc ccgggaggcg tcgaactcgc gatagcccgt gcagacgatc acgctgccga
     4561 cgtcgacccg gcgttcctcg ccgggcccgt cgttgccgtt gacggcgagc gtcgccgtga
     4621 agttgccgac gaagccgtcg aggcgtacga gctcgctgtt ggtcatcacc gtgatgtccg
     4681 ggtgctcgac gacgcgtgtg atccggtcgg tcaggatgtc gggggcggag tcgaggtacg
     4741 gcgccgtgag gtcgacccgc gcgacgttgc cgcccaggcc cggctggcgt tccacgaggt
     4801 agaccagctt gccggcgccg gcgagctcca gcgccgccgt cagcccggcg atgcccccgc
     4861 cgatcaccag cgtgttgggg cacatgtcca cggagagccg ctcgagcggc tcgtggtacg
     4921 cgacccgcat cacggccgcg cgcaccaggt cctcggcctt ctcggtcgcc cgcgcgccgt
     4981 cgtcgtggac ccagctgcac tgctcgcgga tgttggccat ctcgaccagg taagggttga
     5041 gcccggccgt ctcgacggca cgccggaacg tgggctcgtg catgcgaggg ctgcaggcgg
     5101 cgaccacgac acgctcgaga tcgtggtcgc cgatctcctg cgcgatcatc tcctgccccg
     5161 ggttcgagca catgtacttg taggagcggg ccacgacgac gccgggcatg cccgccgcag
     5221 cctccgcgac ggcgtcgcag tcgacgacct tggcgatgtt cgtgccgcag ttgcagatga
     5281 acacgcccgt ccgtggttca ggcatggcgc ccacctcctc aggccgtccg gagcggtgcg
     5341 gcctgctcgg cgctcggctg ttgcttcccg tcgccccggc gcgctcgcgc gagggcggtc
     5401 gcgcgcacct gagccaggta gatggcgagc gggcgatacg ctaggtgcga ccatttgctg
     5461 aacggcacct cgagcgcgag catcggaacg accagcgcga ggtgcacgat gtaggtgacg
     5521 ttggctgcgg cgtcgagccc ggtgcggtgc aggatgtgct gcaggatccc cgtcagcgcc
     5581 accgcgagga tcagggagag gaagatccag tcggactcgt gcgagtgcct gtactgcgcc
     5641 gccgacttct tcatccggtt gcgcaggaag aggatgctgg agacgacgag gccggcggtg
     5701 gcgaggtagc cgagcacgtg gaccctccag tcgacgctcg gcccgctcgc catctccgcg
     5761 aggaagaaca tgatcaggac gagcatgacg cagtagctca gcatcagcgc caggtgtgtg
     5821 acccacggcc ccttgtgctc gcactggctg tagcgcatct gcgtgaggaa gtgggcgggc
     5881 agcatccagg cgctggagat gtagctgccc gggctcttgg gcaagtcttt gtcccggccc
     5941 atggtgaacc accacatccg ggcggcgttg ctgagcagga acgccagcag gacggccgcg
     6001 aggacccagt cgaagatgtg gatggccgag ctggggagga aggcgttggg cccgtcgtag
     6061 tccgagaggc tcccctgcga catgccgaag agcgtgaagg caacggcggt gatcaccgcg
     6121 acgaggagga tcgccaggag ctcccagcgc cacgagcggt agaacagccg cgagatgccg
     6181 gtccagtcgt accgcgacgt cagccagcgc ctcaggctca tcatggtctc gcccggctcc
     6241 gcgtcgcgcg gacactggtc cgagcactct ccgcagtagt agcagagcca ggggtcgagc
     6301 tgcccttcga gcttctcctc gagtcccatc tgcaggtagc gcatgggctt gcgcgggaag
     6361 acgtaggggt cggccgagaa ggggcagacc gcggagcagt tgccgcagtg atagcacttc
     6421 atcacgtcct cggctccgta cggctcgagc tcctcgatca gcttggggtt tgccctgatc
     6481 gccatgaggc cgcctcctag gacgccggga cgaggctctt ggcgcgagcc tcgatgtcgg
     6541 agagagaaag gctcgcgaga ctctgcaggc cgcgccccgt catggccccc tcgagcgccc
     6601 gcgccgccac cgcggacgcc tgggcgaccg tgtcggggat gtccttgggt ccctggcaca
     6661 cgccggcgac gaacacccca ccgcgctccg tgccggtggg gtcgccgttg tagtccgcct
     6721 cggtgaacca gccgtagtcg tcacgaggga tgcccagcat gcgggccagc tcgtccgagc
     6781 ggtccgccgg gacgagcccg acggcgagca ggaccatgtc gacggggaac tccacgacac
     6841 tgtcgctgag gatgtcctcg cccttgacca tcatctgccc gtcgcgctcg gtcaccgtcg
     6901 ccgaccggcc acgcacgatg aacgtgcctt cgtcctggat gcgctcgaag aaggcttcgt
     6961 accctttgcc gaaggcgcgc atgtcgatgt agaactcgta gcaggcagcg tccggcagct
     7021 tctccttgac gagatgcgcg aacttgagcg agtacatgca gcagacgcgg gagcagtacg
     7081 cgttgtggtt cgtgtctctg gagccgacgc agtggatgat cgccacgctc ttcggcgatg
     7141 cgccctcggg atcgaacacc cactcgtcca ccttcttgcg cttgttgtgc ttgagcgact
     7201 tggtgacgat gtgcccgcct gtgggtcccg aggcgttggt gagacgctcg aactccaggg
     7261 cggtgagcac gttggggtgc acgccgtagc cgtaacggtc gagactggac gcgtcgagta
     7321 cctcgtagcc cgtggcgacg atgacgctgc cgacctcgag ctgcagcacg tcgccccgag
     7381 catcgaggtc gatgcactcc ttggggcact tctttgcgca ggcgccgcac ttctgcccgt
     7441 tcgtctgcac gtaggtgcag gaggcctcgt ccaccaggta ggcattgggg accgcctgcg
     7501 ggaacgggat gtagaccgcc ttgcgctccg cgatgccggc atcgaactcg ctgggcacgc
     7561 ggacgggaca gacatcgctg cagatgccgc aggcgacgca ggcgtcctcg tcgacgcgcc
     7621 gggcgtgacg gagcaccgtg gcccggtagg acccggggac gccgctgatc gactccacct
     7681 ctgagtagct gagcagatcg atcatctcgt gctgtcccac cgccaccatc ttgggggtca
     7741 ggatgcaggc ggcgcagtcc agtgtcggga aggtcttgtc gaacatcgcc atgtggccgc
     7801 cgatggtgcc ggtgcgttcc acgaggtaga ccttcttgcc cccgtcggcg atctcgagcg
     7861 ccgcctggat cccggcgatc ccgccgccga tgaccagcgc ggcggccgtc cacttggtgt
     7921 ccttcggccg cgccgccagg gtgaacggga ccccgaacac ggcgcaggcc gtgatggcct
     7981 tggcgcgctc ggtggctccg tcgccgctca cgccgtgctc ccggaacgac gcgagcctca
     8041 cctcttcggg cgcgcgtagc gcgagagcca tggcgcgcgt gaacgcgggc ttgaagaagc
     8101 cgggcgaatc gcccgcgatc acgatcctgt ccaggtcgga ggcgacgatc tcgtcggcga
     8161 gcgcctctgg gtcgaggcgc ggaagcaggc cgagcgagcg caccacctcg acctgcggca
     8221 ggttcgccgc gtagtgacca acagactcca ggttggcgcc gccgcccgtc tcggcgctcg
     8281 cccggcagat gtagacgccg atcctcatcg caccctccgt cgccgtggct gctcgcccag
     8341 aggcacgaag ccgtcaacga cattcggcct cagaagtcga tactagctag gccctagaca
     8401 gaatcaagtg aatctgagca caaatggggc gaacacccta gacgagcgta cgtcggcgag
     8461 gctgggcacg cccggtgggt gtcgcgtctg acctggaaaa ggcgccgacg cgagcgtctg
     8521 tgacgttgcc cgggcggaac tcgagcggta tcgtttgtgg cggcacatgt gtggctgtct
     8581 agggtagata tcctagtgct gcctcgggag gttgccgctc ccgcggcagt ccgatcgctg
     8641 gaggggcgac gtgagcctgc agtccacact acggtatctg gacccgttcc gcgggcgcgc
     8701 ggcacgccgt cgcatcagcg attcggagcg agcgaccttg cgcatgatca atcagaaggt
     8761 cgccgcgaag cagtcgctga acgacctcgc cgacttcctc ttcgagggca cgcggacgct
     8821 gatcccgtgt gaccgcatcg gactggcctt cgtcgacgag agcggccggc gcgtgttcgc
     8881 tcactacgcg cgcgccgact accagccgct gctcctgacc aagggctata cgggcgacct
     8941 caagggtggt tcgctcgagc acgtcatcag gtgcggcgaa ccgcgcatca tcgacgacct
     9001 cgaggcgcac gccagggagc atcccagcag ccacgcctcg ggtcttcttg tccgcgaggg
     9061 cgtgcgctcc agcctgacgt gtcccctggt ggtggagggc cgcgtcgtcg ggtttctctt
     9121 ccgtgactcc acccgccccc gggcgttcga cgcgcaccac gtgcaactcg agatggcgat
     9181 ctcggagcgg ctcagccagg cggtggagaa ggcctggcgc atcgagcagc tcaccgccgc
     9241 caatcacgcc tatctcgaga tgctcgcctt cgtgagccac gagatcaaga accccatcgc
     9301 gtcgttggtg accgacacgc gcctgctggc cgacggctat ctgggaccgc tcgagccaca
     9361 gcaggtggag aagatggagc gcgtgatcgc gaagggccag tacctgctcg acctcgtcca
     9421 ggactacctg gatctcgccc gtatcgacgg tggcgggctg gagctcgtgc gctgcgccgg
     9481 cgtggacgtc gtggaggaga tcgtggcccc gtcgatcgac ctcgttcgcg cccagatcga
     9541 tcaggcggag atgcgcctcc acctcgacct gcccgccggc gcggtgacgg cggagtgcga
     9601 tccggcactc atccgcatcg tcctcgtcaa cctcctcgga aacgccgtca agtacggcac
     9661 cccgggaggc gagatacgcc tgcgggtggt ccgcgagccg gacaggctcg aggtctcggt
     9721 gtggaacgag ggcaagggct ttctcccgga ggaccggggc aggctgttcc agcggttctc
     9781 gcgcctgcgc tcgtctgggg gggcgaacaa gcgtggtacg ggggtcggcc tctacacgtc
     9841 gtggcggatc atgcagttgc acaacggacg gatcagggca cgctcggacc cgggccagtg
     9901 ggcggagttc tcgatcgagc tcgcgcaacc cctcgacctt ccctccggat gacggcgtcc
     9961 ggcgacacga cgaagccggc gcgcgtgtcc cgcccactgc tgggccgggt cgtgctcgtc
    10021 aacgccctcg tcgtcgctgc tgtgctcgcc gccgccctca tcctgcagac gcgggagggc
    10081 ggcatcgggc cggggccggc cgccgggctg ctggccgtcg gcctggcggt cagcatcctc
    10141 gccaatgtcc tcgtcgtcgg gccggcgggc ggggtcctgc tcgagatcac gcgcgccatg
    10201 gagacgatgc acaagggcga cctcggctgt caggtggagg agcgccgcgg aggcgcgggg
    10261 ctccggcgcc tcacggcatc gttcaacgcg atgtgcatgc ggctcggaag ggagtcgctc
    10321 tcgtacgccg agcggcagct gggcagtgtg gaggaggagc gcagacggat cggccgcgag
    10381 ctgcacgacg agaccagtca gacgctcgcg gccaccctcg tccgcctcga tctctgcgac
    10441 cgggcgatcc aggaccacga cgccaccgct gcgcacgcgc aggtggtgga ctgcaagacc
    10501 ttgatcgcgc acaccatgga agagatcaag ctgctggtct acgacctgcg gccggtgatg
    10561 ctcgacgact tcggcctcgt acccaccttg cgctggtaca tccagtccca tctgcagggg
    10621 gcgggcccgg ccatcgtcac cgacttcgac gacgccggcc tgcgcgtgcc gggtcacatc
    10681 gagaccgccc tctaccgcgt gacccaggaa gccctggcca acgccgtcaa gcattcggat
    10741 gcgaccaagg tgcacgtgcg gctggtcacc aagccagggt tcgtgaccct ggccgtcatc
    10801 gacaacggca gagggttcga cgggggggcc gcgctctacg acagcggcca caccggcgtc
    10861 gggctgctct ccatcaagga gcgggtcgag ctcgccggcg ggacggtcaa catcgagtcg
    10921 gcgaaaggca gggggaccag gctgtacgtc gtcataccgc tggccaccga agagagcgtc
    10981 gaggagatga catgaacgcc gccaagactc gggatgagat caaggtgctg ctcgtggacg
    11041 atcacgcgat cctccgcgag ggagtccacg cgctcctcgc gcgcgagccg gacatcgcgg
    11101 tcgtgggcga ggcggcggac gggcaggagg ccgtcgagca ggttcccagg ctgcgaccgg
    11161 acgtcgtgat catggacatc gtgatgccgc gcatgaacgg gctcgaagcc acacgcctca
    11221 tcagggagcg acaccccgag gtgcgggtgc tcatcctgag tatgtacgac gatcaggagt
    11281 acgtcgtcca gatcatccag gccggcgcgt cgggctacgt gctgaagcgc gtggtcacgg
    11341 aggacctggt gagagccatc cacgaggtcc acgccgggga gtccttcctc tacccgccga
    11401 tcgcggcgac gctcatcggg gactacctgc gggtgaccag gggggaggct gtcggccacg
    11461 ccgggggcgg cgaagccctg accgcacgcg agcgcgaggt cctcaagctc atcgccgacg
    11521 ggaactcgaa ccaggagatc gcggagcagc tcggcctgag ccgcaagacg gtggacagcc
    11581 accgcgccaa cgcgatgcgc aagctcgacc tgcacgacgt caccgaggtc gtcaagtacg
    11641 ccctgcgcac gggattgatc acactggact gacgaagcgg acgtgccggg ctccaagggg
    11701 cggcacgccg gtgcggatca cgtcaggggc tgggcgaggt ctgcagcgag ggcgccgcgc
    11761 tcggcgccca gaccggcgac aacgccctct cggcgaaggc cgtcgatccg ccgccgccgg
    11821 ccggcatgag gtagatggtg ccggtccggt acttctcctc atcgtacgcc gcccaggcga
    11881 tcgtctcccc gtccggggac caggagagat cctggacgaa gacgcccttg tgcctcgcca
    11941 ggacgaccgg cctgcccccg gtctccgtgt cgaagaccca gaaggtcatg cgcccgacgc
    12001 gcttgggagg gacccctgcg aagacgaccc gggtcccatc cgggtcccag gcgtacccgg
    12061 ccgggaactg cgcctccttg gccggcatca ggatcgtccc gtcggcgccc actaccgtgt
    12121 agctggtgcc cacgtcgctg ccggcgcgcc ggtagatgag cgcctcaccg cttggggaga
    12181 tgagaggctc gtcgaacgct cgcgcgtcga cgtcgaagaa ggctttgccc gcgtccagct
    12241 cggtcacacg gccggaggcg cggtccagca cctcgaggtg ctccaggccc gtcttcatcc
    12301 cgtagcggcc cgtcgtgacg tcggtgtagc tgacgaacgc gacctcggcg ccctctcgcg
    12361 aggcgctgac ggccacgccg cggagcgaga cgccgcccgc gtcgagaagc ggcgagaatc
    12421 ccagggcggt ggtgtcgtag acgaggaggt cggcgttctg gccggtggtc ctgggcggct
    12481 cggtgtagcc ggcgacgagc aactcggtgg ccgacagcca ggcgatgctg tccacggcga
    12541 gcacgttggg cgcgtcggcc gtgacctgcc gcgcgtcccc gttcgcggcg tcgaagagcc
    12601 acagctcgct gcgctcgtag gtgccggcga cgcacgcgag ccacttgccg tccggcgagt
    12661 acacgatctc ccggagctgc tcgccgctga actcggcgat gggatggacg ctgccgtcct
    12721 cgtcgatggc ggcgagccgg tccgccctgg agccgacggc gccggcgagg taccggccga
    12781 gcgccgggga gggagagacg gcgacggtgg cgccggcgga cggcaccggc gaccggtcgc
    12841 cggcgacctg cttcttgccg cgcggaaaga gcagcagcgc ggcggcggcg atgaccacga
    12901 tgaccacggc gacgacggac accaccagcc acgccggggc gcgcctgcct ccgggtggcg
    12961 gcaacggagg cgacgcccgc gtctccctcg ccccttcggc gagggcggac ccgcacccct
    13021 ggcagaagct cgcatcgtcg tcgttggcgc gcccgcaggc cgggcaggtc cgggccattc
    13081 cccccctcca gagatggtcg gccggtcgtg agggcatcgt atccgctcgt gatgcagcgc
    13141 gacaggtgcg gcgccgtatt cccgtcgcgc cggactccac cccgcccgtg aacgcggccg
    13201 ctcgtccgca ggccgctccc tcgtccggcc gcgctcagcc ggcgggtgcg ggcttctccg
    13261 gcgcgagcgc gccgcgcacg cggccgcggc cggcgaacat gaggccggcc gcgacgagcg
    13321 ccgccgccgc cccataggcg aacgtcgccc acggccccca ggcgctccag agcaggccgc
    13381 ccacggtgct cgcggcgaag gtcgcgaggc cggtggccgt ggagtacagg ccgagcgcgg
    13441 aggcgcgctc tcccgtcgga atggcgcgtc ctatcagcgc cttgctggtg ccttcggtcg
    13501 cggcgatgta gaggccgtag gccgcgaaca ggaccgcgag cgtccagccg gaccccgcgg
    13561 cggcgaagcc cgcgtacacc gccgcgaaga ccaggtagcc ggcgatcacg accgggtact
    13621 gccccacgtg gtcgctgagg ccgccgagcg gcagcgaccc ggctgcgtac acgacgttgt
    13681 agagcacgta gaagaggatc accgcgctcg tgctcatgcc cacgtcctgg gccttgagca
    13741 ggatgaacgt gtcgctggag ttgcccgcgg cgaacagcag cgaacctgcg agcagccagc
    13801 ggaacgcggg cgaggtgggc agcgacagcc ggaacgccgc gcgctgcggc gcctcccgat
    13861 gctccctgac caggaagacg atggcgacga cgctcaggac cccggggacg acggcgacgg
    13921 cgaagatcca gcgcagcggg acgtcggcct gcaccaggac gagcgcgacg agagggccga
    13981 ggaccgcgcc catcgtgtcc atggtgcgat gcaggccgaa cgcgaggcct tgctgcccgg
    14041 ggcgtgtgtt ggccgcgatg aggtcgtccc gcgacgccgt ccgcatcccc ttgcccaggc
    14101 ggtcggtgaa gcggccggcg agggccaggg gccagccggc ggccagggcc acgacgagct
    14161 tgccgagggc gccgaggctg tagccgccga gcacgaaggg tctgcggcga ccggtgtagt
    14221 cggcggcctg gccgaaggga tacttggtga tgctcgcggt ggcttcggcg atgccctcga
    14281 tcaggccgag cagcgcgacc ggggcgccga ggacgccggt cacgaagatc gggaacagcg
    14341 gataggcgat ctccgagctc aggtcggcga gcatgctcac cagaccgagc ctgcgcacgt
    14401 tcgggtcgag ggtgcgcagc tgcgcgaccc agcggcgggg cgaagggcgg atcatggagg
    14461 gctcgacgcc agcagccgtc cggctggaga gccgggaagc tcgcgccgct ctgcggtcct
    14521 ctcagaaccc gccggggccg aggtaggcgg tgaggccggc gtcggcctcc tcgtagatgc
    14581 cccagacctt cttgacgggg ctcaggttct tccattcccc gacgctgccc ttgggggtga
    14641 tgacgatgtc cccggggccg gcgacgtgga cgtcaccctc ggcatcggtg atctcgacct
    14701 cgccctcgat gatgtaggcc acttcgtggt agggacgctc gaagtggcgt cgctgcacgt
    14761 cgcgcttcca gagcccgaag ctgaacttgt cgtcggccga catcgccgcg gtgagttcct
    14821 tgcccggcag gtcctgctcc cccgggttca gcatcgcatc gtcccaggtc acttcgtccg
    14881 gtttgatcac gcggatcctg gtgtcgctca ggtcgcccat ggcctccccc atcgagtgcg
    14941 gcgtcgtaga atagccggtg cgcccgcggg gcaccgccag tcgagccagc tgcgccgtcc
    15001 gatcgcggcg ccgctccggc gccattgtac gcccggcgac gccgcaggtg tccgtctatg
    15061 gaggaggtcc ggggtgcagc tgcagtcggc gctcgaagag cgcgtcgtcc aggcggtacg
    15121 ggacgcgaag gacgaactcg tcggccttgc cgcggagctc gtcgcgctgg atacgaccgc
    15181 acgcctgccg ggcgacccgg cgcgcgacga ggagaagctc cagcggctgc tcgcggcgcg
    15241 gctcgaggca ctgggcgcag cggtggacct gtgggagccg gaaccgaccg gggacggcga
    15301 ccggttcgtg ccggccggcc tcgacttccg cggccggccc cagctcgccg cggtcctgcc
    15361 gggttccggc ggcggccgca gcatcctgct caacggccac atcgacgcgg tggacgcgga
    15421 gccgcgcgac gagtggctca gcgacccgtt cgtcctcacg gaacgagatg gctgcctctt
    15481 cggtcgcggc gccaacgaca tgaagggcgg gatcgccggc ctggtcatcg ccctggagac
    15541 actgcgccgc cagggcgtcc gcctggccgg cgacgtcgtg tgctgcacgg tcaccgacga
    15601 ggagtcgtcg ggcgccggcg gtcgggcggc ggtcgctcac ggcgtcaggg ccgacgccgg
    15661 catcgccgcc gaggccaccg acttcgacgc ctgggtgagc tgccgcggca cggtgacgcc
    15721 gacgatcacc gtggccggtc gcgccggtca cgcggagatg ccgcagccgg actggcgaga
    15781 gggcggcgcg gtcaacgcca tcgagaagct cgtcgccgtc gtcgacggca tgggcgcgct
    15841 gcgggcggag tggaaggcgc gccccgacca cgtgcacccg tatctcgcgc ccggcgacat
    15901 cgtgccgacc atcgtccacg gcggcacctg gatggtcacc atcccggcgt cgtgcgccgt
    15961 caccgccgac atcatgtacc tgccgcaaca cgtcgacgcg gaggggactg gcagggcggt
    16021 cgaggccgaa gtgatcgacc gcctcaccag ggcggtcgcg ggtgacccct ggttcgcgga
    16081 gcacccgctc cggttcacct ggtcggacga cgtcgtgccg gcggagatgc cggccgatca
    16141 cccgctcgtg accacggcgc tcgccggcgc cgcgacgctc ggtcaccgcg gcgagccggc
    16201 cggcctgaac agctggcacg acgccgccac cttcacgcgc gccggcacgc cgaccttcag
    16261 cttcgggccg gacggcttcg acacggcgca cgccgtcgac gagcgcgtca gcgtcggggg
    16321 gctcgtcgac ttcagcgccg cggtcgccct cacgctgttg cgctggtgcg gggcgtcatg
    16381 atcgtccatc tgtcgatcca cacgcccagg cccggaaggg agcgggacct gatcgactcg
    16441 atgcaccgtt acggcgccgc gggcgccggg ctgcccgggt tcatcgacgc gaagacgctg
    16501 cgcgaccggc acacggggcg cctggtgggc atggcgcgct gggaggatga ggcctcgtgg
    16561 cgcgccggcg tggaggcgat gcgcgccgcc gtcgcggacg acccgttcga cgagtgggag
    16621 gcggccgagg tcgaggggtt cttcctcgag gaggtgtgag ggtggcgacc acctccggcc
    16681 aaagggggcg gggtcggccg ccggcgcgga cgccggcgca gccgtgaccc cggcgccgac
    16741 ccgcgaccgg cgccgcgcgc tcgtcgccga cgcggcgctc ctcagcgtcg ccatcgtctg
    16801 gggcctcaac ttcgtcgtca tcaaggacgc catcgccgac accggcccca tgacctacct
    16861 gctgtggcgg cacatcctgg cggccgtcat gctcgccgcc gtcatgccgc gcaccgtcag
    16921 gcagaccggg cgccgcgact ggctgtacgg cgccgtactc gggctcttcc tcttcatcgc
    16981 tttcgtcacc cagaccatcg gcctgcagtg gaccaccccc ggcaagagcg gcttcatcac
    17041 cagcctctac atcgtcatgg tgccgttcct ctactggatc gtcgcgcgcc gctcgcccgg
    17101 ctggacccag atcggcggcg ccgtcatcgc cacggtcggg ctcggcttcc tcagcttccg
    17161 tggcggcatc ggcatgtcga ccggcgacct gctcacgctg gcgagcgcgc tcgccttcgc
    17221 tgctcagatc gtggcgacgg ggttcttcgc cccgaggacg acaccggcgg tgctggcgct
    17281 cacgcagatc gtcgccgcgg ccgtactctt cgtcgtcgtc acgccgttcg tggagcacat
    17341 cacctgggac ttcggccggc aggcgtgggc ggcgatcgtg tggacggcgc tgaccggcac
    17401 cgtgttcggg ttcctcgtcc aggcgtgggc gcagaagtcg acgacgtcca cgcgcgccgc
    17461 cgtgatcctc ggcctcgagg gcctgttcgc cgccgtgttc ggccttctct tcggcatgga
    17521 cgtgctgagc tggcgcttcg ccgcgggcgc ggcgctgatc ctcgccgggg tgatcgtcat
    17581 cgagacgctg cccgggcgcg gcgcgcccaa cccggcggac gtgctgccgc gcgccgactg
    17641 acggcgagga gcgatcagcc gcctctggcg cgaccggctc cctctggtac tcttgcgggg
    17701 tacggctgtg ccatcgagtc gggagcccgc atggactggg actgggaaga caggccgccg
    17761 aaggatcccc cgcagcctcg gcgcgcccct gatgggccag acgacgagcg cgatctcgct
    17821 cagcccgaga taccgtccga ggccgccgcg cccgaccctt cgcctggccc cgagccgcag
    17881 gagccggagc ccgagcgccc ggaggacgcg tctcccttcc aggagcttcc gcctcttgac
    17941 cccgcgcccg actacgtgct gcccgggcgc ccgggcagtc cgacggtggt cgagtcgccc
    18001 ggcgcggagt ccggccggcc tccgaccggg acgatcgaag cggccgagcg agcggtcgtc
    18061 ggagccgagc gacgcgaccg tgaccagtac cggccgcact cggccgagga ccgtgccgcg
    18121 gcgcgggcgc agcgccgccg caagctccgc cagcggcggc tcatcgccct ggcggttgcc
    18181 ctggcgatcg tcgtccttct ggtggtgctc gtcgtccgcg cctgcggggg ccctgatgcc
    18241 gccgcggcgg tcgccgcctt cgccgtgccc ttcgtgagca gacgcctcct ggtggccacg
    18301 gagcgaccgt gaggccgggg cgagtggccg cccgggcact cgtcgccgtg atcgcgtcgc
    18361 tcgcgctcct cgccggctgc ggcggcgctg gaccggagac ggcggaccgc cccttcgggg
    18421 acccgccgcc ggcggttccc ggcatccacg cctgggccgc cggtgaggcc ggcgtcctgc
    18481 tcgtcaccgc cgacggcggc gccagctgga tgcgtcggga gttcttcctg ccccagcgcg
    18541 gcgtggacgt cgccttcgcg gacgcccaga ccggctggct ggtcaccgac gccggaacgg
    18601 tgctcgcgac caccgacggc ggcacgggct ggacggtcgt cgagcaggcg gagctcaccg
    18661 ccaaagccat cgcggcgaca gactcccgcc gcgcctggat cgtcggcagt ccgtcggggg
    18721 ccgccgacga gcccggggcc tcggccgtgt tgcgcacgac ggacggcggc gaagtctgga
    18781 ggcgcacggg gttcggcacg gcccagctcg ccgacgtcgc cttcgccgac gagcgtcgcg
    18841 gcgtgctcgt cgcgctggac aggatctggt cgacgcgcga cggcggccgc acctggcggc
    18901 tccgccgcga gctgcccatg accgtgctga cgagcgtggc cgccggcgac ccccggcgct
    18961 tctgggtcgc cggctggggc acgcaactgg gtgacccgct cgtgttcgcg acgcgcgacg
    19021 gcggcgccac gtggcgacgc ctgcgcgtag acgtggcccc ggcgaatccc ggcgaactgc
    19081 aggccaggca gatcgcctgt gccggcgaga gtctctggat cacctgcgcc gccggcgtgc
    19141 tcgcctccgc ggacggcggc aggacgtggg agctgcaaca ggtgcccgca gggcagccgc
    19201 tggccatcgc cgccgccgac gaggagcacg tcctggcgac gaccgggagc cagccgatcc
    19261 tcgccagcgc cgacggcggt gccacgtggc gcgcgttcgg ctcggagggg ttcctggagc
    19321 agccgctcgt cgccatcgac gcggtcgtgg cgcagccggc gcagtaggcc gccgcctcgc
    19381 cggccgccgc ccgtccgata ggattgccgc gatgacgacg ccgaccggca gcacgctcgt
    19441 ctcgcgccgc agggcgctcg tcgccgacgt gctgctcttc agcgtcgcca tcttctgggg
    19501 cagcaacttc gtcttcatca aggacgtcgt cgagcgcacc accgagctcg ccgccggcag
    19561 catcgtcgcc ggcacgatgc tgtacctggt gctgcggtac gtggtcgcca cgggcatctt
    19621 cgccgcggtg cagccgcgca gctggctcga ctcctcacgc ggcgactggg cgcggggcgg
    19681 ccttctcggc gcgttctacc tgaccgccct catcctccag acgatcgggc tccagcgcac
    19741 ctcgcccggc gtcagtggct tcatcaccgg gatgagcgtg gccatggtcc cgttcctcta
    19801 ctggttcgtc gcgcgccgct ctccgggccg ctggcagatc atcggtgcga tcgtcgcgac
    19861 cgtagggctt ggggcgctca gtctccaggg ggacttctcc atccgctggg gcgacgcgat
    19921 caccctgctc ggcacgctgt tctacgcgct gcacatcatg accacggggt tcttcgcccc
    19981 caaggtgaga ccgaccaccc tcgccgtgac gcagatggcc gtatcgaccc ttgtcctggt
    20041 cgtcgtcacg ccgttcttcg tgcgcatcac gctcgacctg ccgtggcagg tgtgggcggc
    20101 cgtggtctgg acggcggtca gcgggaccat ctacgcgttc ttcatccaga gctgggcgca
    20161 gcgctacacg acctccaccc acgcggccat cctgctcggg ttcgagagcg tgttcgccgc
    20221 cgtcgcgggc atcatcgccg gcatggactc ggtgacgtgg cgtctgctgg tcggcgggtc
    20281 gatgatgctc accggcgtgt tcatcgtgga gttgctgccg gggagcaagg cgatcgttga
    20341 ggagatcgag gcggaggagg ctacgtagag acgcgacgca ggatgtgccg ctcggacccg
    20401 ctcgggctcg cgaggagcag tgcgtccgcg ccctcgtcgt aggtgatgac ccagcgcacc
    20461 tcctgcgtgc cctcgcccca gacgaggatc cggtcgccct ccaggcgcgc ggggagaggc
    20521 tctgcggagg tgggcgcgta ggtcacccag taccgggcca cgtcggccgg tgcgtccgcg
    20581 gcgctcaccg tgaggcggat cggctgcgac gtggtctgct cctgccacac gccgagcgcc
    20641 ttcgtctgga tggggggtcc cgcgtcggcc tgccgccaca cggccgccgt gatgacggcg
    20701 acggcgacga ccaccaccgc caggatcggg atgaggacga cctttctcga cacggcaagc
    20761 cctttcgtgc ctggggccgg agccctctac tcctcttctc tgcgccggcc gaacagcctc
    20821 gtgagcacga acatcacgac gaggaggatg agcacgccgc cgagaccgac ggccagccag
    20881 gtccagttca cgatgggctc gtcctcggcg gtcgcggcgg cggccacttt gggctccacg
    20941 aagcggtacg tgtcgatgtt gttgtagctg tagatgacgg cgccctcctg gccgctcgcc
    21001 tcactcggca cgtgcaccca gccctgccac tcgtcggtgt tgaaggcctc cagctggtac
    21061 gggtaggcga ggatggcgta cccggcgccc tcgtagaaga tctgctgcat ctgctggacg
    21121 atcgggacgc gctgcgcctc atcgatggtc gtgatctgct cgtcgttgag cctggtgtac
    21181 tcggggtccg tccacaggca gtcgttccag ccctcgatct gctgcggcgt gtagatgtcg
    21241 acgatgaagt tggggtcgac gtcctggacc cagtaccaga tgaacaggtc ccagttgggc
    21301 gtgtacgtgt cgccggtgta ctcgtactgg tagttgatga gcgtgccggc gtcgatcacc
    21361 tgcagttcca gcttgatgcc gacgtcctcg agccagccca cgatgagctt ggccgcggtc
    21421 tggttctgga cggagtcggt ggtcgcgtag aagcgcaggc tgagcttctt gccgtccttg
    21481 gtctcgcgga agccgtcgcc gtcgacgtcc tcgtagccgg ccgcgtcgag gatctcgttg
    21541 gccctggccg ggtcatacgt gaaggcctga tcggccggca cctcccagtg gtacttggag
    21601 taggggacga tgagcgtgga gccgagcgtg gcgtactgct ggtaggcgac gtcgacgacc
    21661 ttctgccggt cgaccgccca gttgacggcc gtgcggaact gctggtcctt gagtacgggg
    21721 ttgcctttgg agttcgggct gtcgtagcag ttcatcgcca gctcgatgaa gttccaggac
    21781 gtggactggc tggtggtgat gccctcctcg tcgcccagcg gcttgaactg cgccggcggg
    21841 atgtcgacgg cgccgtcgat ggtgccgagc ttgaggtcct gggccatcgt gtcggggttg
    21901 gtgtagagct ggaacacgac ctcgtccacc ttgggcctgc cggcccagta ggtcgggctg
    21961 gcctcgaagc gcacgaactt gcccttctgc cactccacca gctggaaggg cccgttgccg
    22021 atgatgggcg gcttgttctg gtagctggtg gtcgcctcct tgccgctgac cttgctccag
    22081 atgtgctccg gcaggatggg gacgaccatc cgcagcatgt tcgccttggg tgcggcggtc
    22141 atgaccttga cgtgcgtgtc gtcgatcgcc tccgccccgg tgatgccgtt ggtgtacgtc
    22201 gagaggttca gcagctcgtt gtcgacgatg tagttgaaag tgaacgccac gtctcgggcg
    22261 gtcaccggct ggccgtcctg ccacgtggac ccttcgcgga tggtgaaggt ccactccttg
    22321 ccgtcctcgg agacctccca ctcggtcgcg agctcgggac gcggctcgag cgtcttggaa
    22381 tcgaagccga cgaggaagtc gtagttcatg tgccagaaca tgtagtcgat gccctggatg
    22441 ccgatgaacg ggttcaggtt gtccggctcc tgcaccatgc cgatgtgcag cgtgaccttc
    22501 tcgccggggg atgggctgga ggaggcggcg gcgcgggcga cgccgcccgc cgccgcgagc
    22561 accagcagca ggatcaggac catcgacacc ttgatgagcg gacctgcctg ccgggtgcgt
    22621 gctgccatgc tcgttccccc ctggtgcggt tggaccggcg tcgccaggcg ccgctcccct
    22681 gtgggccgac gatatggacc ggccgcgggg gcgtcaaggt acgccgcggc tgccggtccc
    22741 tccatttcgc aaggtagact gtccgggcgg cggggcccgg agggccgatc cgcctcatca
    22801 gcgtcgccgg ttcacgggag gccacatgcc cgacacgcca tacgaagacg tccagatcga
    22861 gggccacctc atcgactcgc tcatgatgcc gcacatcatg gacgaggtga tggacctcga
    22921 cggcgagttc gagatgctcg ccttcgaggt cgggcgcatg aaggacgatc cctcgcacgc
    22981 ggtgatgcgc atcttcggtc gcgacgccgc gcacctggag gagatcctca cggcgatcac
    23041 cgagttcggc gtcgtcgccg tcaagccgca ggacgtcgtg ctcgaagagg ccgacatcga
    23101 cggcgtcttc ccggacgcct tctactcgac gaccaacctc acgacgttcg tgcgcgtcgg
    23161 tggcgagtgg atccgcgtcg agcatcccga gatggactgc ggcatccgcg tcgacgtcga
    23221 ggcgggcgct gcggaaacgg tggccatgag cgacgtgaag gccggcgacc gcttcgtcac
    23281 cggccaccgc ggcatccgcg tgctcccgct cgagcggccg cgcgagagcc agccgttcga
    23341 gttcatggcg tcggccgtga gcagcgagaa gcccaaggcg cagatcgtgc acgaggtggc
    23401 gcacatcctg cgccaggtca aggaggaggg ccggctgacg atcgccgtcg tcggcccggc
    23461 cgtggtgcac acgggggcgg cgccgcagct ggcccgcctc atcgaggccg gctacgtggg
    23521 ctgcgtgttc ggcggcaacg ccgtcgccac ccacgacatc gagagcaacc tctacggcac
    23581 gtcgctcggc atcaacctga agtcgggggc gcccgtgccc ggcggccacg agcaccacct
    23641 gcgcgccatc aacaccatcc gccgcgccgg cagcatccag gctgccgtgg acggcggcgt
    23701 cctgaccggc ggcctcatgt acacgctcac caagacgggc acgccctgcg tgctcgccgg
    23761 cagcatccgc gatgacgggc cgatgcccga ggtgatcacc gacgtgatgc aggcgcagaa
    23821 ggagatgcgc cgctacgcgc agcaggccgg cgcctgcctg atgctgagca ccatgctgca
    23881 cagcatcgcc gtgggcaaca tgctgccggc aagcgtgcgc acggtgtgcg ccgacatcaa
    23941 ccccgcggtc gtcaccaaac tggccgaccg cggctcgttc caggccatcg gcatcgtcac
    24001 cgacgtcggc ctgttcgtgg agcagctcgc ggacgaactg acgcggtagg cgccgccccc
    24061 accgcactgg gcggggcaag ctcactgctc gacggcgcgc cccctgcggc ggagcagcag
    24121 cacgatcgcg gccaccgcaa gcgccgccgc ggcgaccccg gctatcagcg cggtgtccgg
    24181 cccctcggtc tcggccgcgg tctcgctcgc ggccctgggg tggacctgca cgtaggagtc
    24241 gatgttgtcg ttggagaaga cgaccatgcc gttgtctccg gggtagggga cccagccctc
    24301 ccacttggcc gtgttgtagg cgatcagcgc cttcgggtag tagaggacca tgtacggagc
    24361 gtccgcgtag aagatctgct gcatgcgctg cacctgcagc tcgcgcttgg gccagtccat
    24421 ctcggcgtcc tgctgcacga agagcccgtc gtactcggcg ttggaccagc agcagtcgtt
    24481 ccagccctcg atctgggacg aggtgaagaa gcccagctgg tagcccgggt cggcgatact
    24541 gccccagccc cagatgaaga ggtcgtagtc aggagcgtag tcttccccct cgtagtggta
    24601 gacgccgtcg acgagcgcgc cgtcgttctc gacgctgagc tggatcttca ggcccagctg
    24661 cccgagccaa ccggcgatca gcttgccggc gcgttgctgc tcgggtgatt cgctgcgcgt
    24721 ccacaagcgc aactcgatgg gcgcgccctg cttgtcctcg cggatgccgt caccgtcggc
    24781 atcccggtag ccggccgcgt cgagcatggc cccggccttg gtgagatcga agccaaaggc
    24841 ctcctcggcc gagggcgccc acgcgccgtc ggtccccggc acgatgatcg actgtcccac
    24901 cgccccgtac ccgcccagac aggtttcgac gatcgcgtgc ttgtccacgg cccagctgat
    24961 cgcccggcgg aaggcgacgt cccgcaacac tgggttcgcc agcgagtcct cgtcctggta
    25021 cacattcatt gcgacctcga cgaagtaccg gatgtcggcg gccttggcgg tgatgccggg
    25081 cgcgttctgc agggctgaga actgcgcggc aggcaggtcc tccgcgacgt cgatggtccc
    25141 actcctcagg tccatcgcca tcgtgtcctg gttctggtag atctcgatga tggcctcatc
    25201 gatcttcggc gcgcccttcc agtagtcggt gttggcgacc aggcggacgt aggagccctt
    25261 cttgacctcc acgacctgga agggaccgga gccgatcacc ggtggctcgg gggcaaacgt
    25321 cgccgcctcg tcccccggca ccttcgccca gatgtgctcc ggcaggatcg ggatcggcag
    25381 ccgcagcatg ttcgacttcg gccgctccag gcggaactcc accgtcgtgt catcgacggc
    25441 gacggccttc ttgatgtggg tggtgctgcc ggtgaaggcg gagagctcgt tatcgatgat
    25501 gtagttgaac gtgaaggcga cgtctcgcgc ggtgagcggc acgccgtcct gccagcggat
    25561 tccggggtgg atgcggaagg tccaggtgag cccgtcctcg ctcatcgccc agctgtcggc
    25621 gatctccggg cggacgtcgc cgtccggcgc gctgccggtg agcatgtcgt agttgaggtg
    25681 gtagacctcg taggcgacgg aggtgtagcc ggcgaatgga ttgaggctgt cgaggtcttg
    25741 agtcgtaccg aggcgcagca cggtcgccgc cggggccggc gacgaggacg caccggcgct
    25801 cgcgccgctc ggcatcacga gcagcaacgc agcgcaggcg actatgatga ggaaacggcc
    25861 cacgcgcgac cccttcgcgc ccaccgtccc caccctccac agggggacgc tggcgcgact
    25921 gtataccttg gtcagcgttc gtgtcgccgt tttcgcggcg cttcgcgcga gagggcggca
    25981 cgcgagcgcc cgcgcccggg tacgtggaat cacccgtatc gggtgatgcg gcgcaccctc
    26041 gcaacggcaa cagtcgcggc acagcgatca gcggtcgccc cgccgcgacc gctgcgagga
    26101 ggctccgcat gacagacgac cacacgctgc gccccagtcc ccaccgccct cccgagcccc
    26161 ccgaaccacc ggagcccggc cggcccaacc ctcacaggcc accggagccg ccggagccgc
    26221 ccgagccgcg agtgggctga gggggcgcta cggtctcgac ggcggagcgt gcccccgacc
    26281 gggcgcctcg cgtgctgctc atcgaggcgc ccttcgagcc gctgcagcgc gggcgcctcg
    26341 cgcctgcgct gctcaaggcg gagctgcagc agcgcgggct gaggtgcgac gtggagcacc
    26401 tgggccacgc gttcgccgcg ctgcttggcc gcagcgagta cgcgcgcttg ataagggagg
    26461 tccctccggg ggcgctcgcc ttggactggg tgttcgccga ggccctgttc gggaccgggg
    26521 tgccggctcc ccgcgcctac gtggccgacg tgctggccgg ccactggcgg ctctcgggcg
    26581 agcttgtcga gcttgtactg cgggcccgcg ggctggtggc aggcttcctc actcgcgcgc
    26641 tcttggagat cgactggtcg cgctacacgg tgctggggtt ctccgcgctg gtcgggcaag
    26701 cggtgtcttc gctcgccttg gccaaggcgg tgaaggaggc ccatcccggc gtgcacgtcg
    26761 tgttcggtgg gcctgcctgg cacggcgtga tgggccggcg tcagctggcg gagttcctct
    26821 tcgtcgacgc cgcctgcgag ggcgacggcg accttgtgtt tcccgcttac tgcctgtggc
    26881 tggcggcagg atgccgcggg ccgtggcctg ctggcctcct gctgcggtct tccggcgggt
    26941 caggaggcca gccgccggta tctccgtcga tgagcgacct cgacgccttg ccgacgccgg
    27001 actacacgga cttctaccgc gccctggcgt tgtggggcgg cgacgccggc gtggcgcaac
    27061 tcgccgccga ggcgtctcgg ggctgttggt gggcggcgag acggccgtgc gcgttctgcg
    27121 gcctgaaccg acccactcgt cgctatcggc ccaagtcggc cgaccgcatc ctcattgaac
    27181 tgcgagagct ggaccggcgt tggcagccgt ggctcatcga cctggtggac aatgtggtgc
    27241 cgcccgcgtt cctccgcgag gtgctgccgc ggctcgccgc gtcacccctg acggcgcggc
    27301 tgttcttcga ggcgcgcccg gagctgacgc gggcccagct ccgactggcg gctcgtgccg
    27361 gcgcgcacgt ccagctgggc atcgagagcc tcagccagca cgtgctcgat ctgctgggca
    27421 aaggcacgga cgcacgcctc aacgtgcggg agctgaagtg gtgtcgcgac gagggcgtcc
    27481 ccgtgacgtg gaatctgctg cacggcatcc ctggggagac ggcgcgagac tatcaccaga
    27541 tgcgttcgct cttgccgctc ctgcggccgc tccccccgcc tgaggggtgt ggggctgtcg
    27601 aggtgcatcg gttcagccgc tacttctcgc agccggctgc gttcgggatc gtcaacgtgc
    27661 ggcccgcctc cgcgtacagt tacgtctacc ccttcaacgg ggcgaagctc cgagacctcg
    27721 cctacctctt cgaccatgag ctcgtgcagc ctgttcgtgg ggatcggcag ctccaacgcg
    27781 agctctccgc gttcctgctg caggcgcacg agtggattgc ctccgcgact gcgcctcgtc
    27841 aagctgcccc gcctgtctga gtcatcaggc cggcggcctc ggccatggcg gccggcatgg
    27901 gcagctcggc gaacaccgcg tgcgcggccg ccaagggctc tgcggcctca gcgcttcgct
    27961 cgatccttga aaggcagcgc cccagcccaa gctgggactg ggccttctcg gtaacgaagg
    28021 ccagcgctgc ccagcgtacg acggcatcgc ggtaggcgtc cacggcctcg tgccaccggc
    28081 cgccatgctc ggcggccagg gcgcgccaca acgacaggac ggcaatgtcg aagggccggt
    28141 gggacaggcc gtcgaccagc cgctcagcga gggcggacgc gtcgagtgcc gtgagggtgc
    28201 gcagggcgaa aggcaggccg gtcgcgatcg cgccacgttt acccctggtc gcctgttcga
    28261 gcgtggtgag ttccgccagc gcaccctcgt cgtcgctgag accgtggtgc acggcggaga
    28321 ggaagcacga gcatgtccag gcgtggtttc cgaaggcgac ggacgcgatt gttccctccg
    28381 cctggcgtgc cttctcgcgg gccggttcga tgtcgccccg caagagcgcg agcagcgcca
    28441 tcatcgagcg catctcaccg agcatctgca ggtcccccgc cctctccagc gcggtctcca
    28501 gggaaggccc cgcgaccagg acctcatccc atctgcccgc acacaggagg tcgaagcaca
    28561 tgcctgcttg gagggcgttg gccccgttgg catcgccgcg tctcaccatg tcgtccacgc
    28621 cggcctgaca catcgcaatg gattgctccg tgccttcgta cgtcatgagc gccccggcgt
    28681 agttgtagta gagagacgtc aggtcccggc ttcgcccctg accgatcgct tgatcgagtg
    28741 ccgtggcgag atcggtcaag ccccctgcat ccccctgacc gcaccgggct gtgcctcgcc
    28801 agagcagcgc tcgcgccggc atcggcgtgt cgagggacgc ggccatcgac agagcggcat
    28861 cggcggcggt gatggcgtcg ctgtggcgga acgcgaagac gcaggtcatc gcgtgctcct
    28921 cgagggctgt gacgagcgtc gcggacggac cgtcttgccg gagcatctcg agcgtcgccg
    28981 aagagacctc gggggcgtcc ggatctccca tccagttcat gagtctggtg agtcgcgttc
    29041 gcccgtgggc cgccacgggc cggtcacccg cttcctcggc cccacggacg ccctcctcga
    29101 tgcaggcgcg cgcctcgtcg agcctgccga tatggctgag cgcgtccccg cgctcgatcg
    29161 tgacccaggg gcggcgagga tgcccggtgg gcatgagcgc cgccgcggcg tcgagatgcc
    29221 gctccgctcc gcgcatgtcg accggctgcg tctgcagcgc cgcccggatg tgtgcctcca
    29281 cggccggctc gagcagctcg gcggcaagcg ccgcgtctgc gtgtcgcgcc gcctcgagcg
    29341 cggtggtcag gtgatgggcg atcagcgccg ccggtgacca ctgcccttcc tggtgctcca
    29401 gccagtcggc cacgcctttg tgtcgggacg cgcgcgcgag acgcggcaac atcgcgtacg
    29461 tcacgtcccg caccacggta tgccagaagg cgaactccgc ggccgtggcg ggggagccgg
    29521 ccatcggccg cacgagctca cgctcggtga ggcgcgtgag ggcctcgtcg acagcggcgg
    29581 cgccggacga gtcgagagca tgcagcgcac cacgccagaa cgtcgtcccg acaacggccg
    29641 cgttcgagag cacggtgcgc tcgacggcgt cgagtgtctc cagtcgcgcc gcgatcaggg
    29701 cctgcagcga gggtggaggc agatctggtc gcatttctct cttcgggcct gcgccggtgg
    29761 cgcggtcggc cagaaatcct gccatctcct cggcgtagag cgggttgccg ccgcagctgc
    29821 cgacgaccgt ggccgagacg tccgcgtcgc cgtcgtgcag cagcagcgac acgaggcgct
    29881 cggtctcgcg ctccgacagc ggatgcagag cgagcgtcat cgggggcggc gggtcgccgg
    29941 cgacggcagt ccgccggtcc cccgggccgt cgagcagctc gggccgcgcg gtcaggatcg
    30001 tgagcagggg cacgtcgtcg atcgtggagc ggaggtgctg caggaactcc gccagcgtgg
    30061 agctcgccca ctggatgtcc tcgaccacga gcaccgtcgg gcgcacgcgc gccatcagct
    30121 cgatgaaccg cgaccacgcg gcgaagttct cggaactcga cgcgcggtcg ccccctgagc
    30181 ccagcagcgc gcgcagacgc tggaggaacc acggttcgtc tcgcgcctcc gcgaagcagc
    30241 gttcgagctt ggtctggacg gccgcgtcgt cgtcgccggc gacgacgccg gcgtgctccc
    30301 gcaccacctg cgccagcggc cagagccccg ccccctcttc gaacgggctg catcgcacct
    30361 gccgccactg ggcgagcaga tcggggcgag cgtcgaggcg ccgtgccaac tcgacgagca
    30421 ggcgggtctt gccgatcccg gcctcgccga cgaggaggac ggcctgtggc gacgcggagc
    30481 gcatggactt ctcgaagagt cccgtgagga tgccgagctc gacctcccgc ccgacgaagg
    30541 gagcagcctc cggccgctgc agctgtacgc cgagctgcgc cacggtgccc gtgacgagcc
    30601 acggccgctc cagcgcttgc cggcccctca gcctgacggc cggcagcgcc gtgaactcgg
    30661 tgacgtgcgc ggtcatggcg tgcgtcgtct cgccgacgac gatgccgccc ggcggcgcca
    30721 gcgccagcag gcgcgccgcc gtgttcacgg catcgcccgc ctggaagctc tcgccggcca
    30781 gggggtcgac gtcgagctgc accagagcgt gaccggtgtt cacgccgatg cgcgcgagca
    30841 caggctcgcc ctgatggtgc ggcagttcgc cgagacgcag ctgcagccgc acggcagcgc
    30901 gcacggcccg ctccgggtcg tcctcgtgtg cccggggcac gccgaacacg cccaccacag
    30961 catcgccgac gaacttctcc acgacgcctc cgtgctgctc gatcaccccg cgtgcgagcg
    31021 catagaaggg gcgcaggagg gcgtcgacgt cctccgggtc gctgcgctcg gccatggccg
    31081 tgaactccac caggtcgcag aacagcgtgg tgatgaggcg gcgttcggtg cgcgcggccg
    31141 gggcgatgcg gcgcgcgccg cacgacgggc agaagcgcgc gccgtcaggc agcggctccg
    31201 agcaggactg acagacgccg tccattcgca cccctcacga ccgctctacg accggtccca
    31261 tctcctccgt gcagtgtagc ccggaggcgc gccgccgtga acggttcgtc cgtgcggcga
    31321 tcacgggtga ccgggtcgtc gccgcaggca tggtcgccgt ggcccggggc gtgctccggg
    31381 ccggtggtac actcgggtcc gtagcgtcct tgtcgtccgc agcggagggg tcatgaggaa
    31441 caagctgcca ctcttgctcg tcgtcgtgct catcgccgcg tgcgccgttg cggccgcccc
    31501 ggcccgtgcc gcggattctc ccgccgccat cgcccgtgag atcctcggtc acgccccgcg
    31561 tggtctcgcg cagacggtcg tcgagcgcgg gtatgtccgc gtcgccaacg acgccaacta
    31621 ccggccgttc agctccgtcg acaccgacag cgctgggctc gtcggcttcg acgtggatta
    31681 cgcccgcgcc gtcggcgaga tccttggcct cgaggtgcgc ttcaagaacc ccgcctggag
    31741 ctgggtgccg cgtgggctgg tgagcgcgcg ctacttcgag gtctcgatcg gcggtctcaa
    31801 gaacacctgg aagaaccggc gcaccctcgc cttcacccgc gcctactatc acctcgccgc
    31861 gaaggtcctg atccgtaggg gagggccggc cgtcaccggc gtggagtcgc tcgccgggag
    31921 acgcgtggcc gcgcagtcgg gcacgtacct gcgcttcctc gagcggaaga ccggcgcccg
    31981 cgcggtccag tatctgcact ggcaggacgc ccgcgccgcg ctgctgcgca gggacgtccc
    32041 catgtggctg ctctcggccg tgaccgccgg acggttggct cgggaggacg accgcctgca
    32101 ggtcatcggc ccggcactct tctgggaccg ctgcgcagtg gccgtgcgca ggggcgagga
    32161 cgacctggtt gagttgctgg acttcgccgt ccgcaggttg aaggacgacg gcacgacgca
    32221 ggcgctgatc gtcaagtggt tcggcgctgg ggacccgggc atccggccgt aggcgaccgc
    32281 ctgaggggag gtccgtgacg cacgagcgcc tcgaggagca gccgcgccgc ccaaggtggc
    32341 cgtggctcgt cctcatcctt gtcgtcgcgg cggccgtcgg agccgtgtcg tggcgctggg
    32401 tgccgcccgg cgacgcgacg atgtcgcagg tgcgcgctgc cgaagtggcg tacgagtccg
    32461 tgttcgcgcc ggtcgcgccc gaaggcgcga agcccggcgc ccccctctcc gttggccagc
    32521 gggcggcgct cgagggcgag ctgcaggcgc gcctcgaggc ctgctgcacg cgggacttcc
    32581 tgcggacgca gggccgctac gcgcggtcca tggccaagcg cgtcagcacg gccctcgcga
    32641 acgggacacg ctacgacccc gtccacccct acgaacacct gcgcgacctg cagttccgcc
    32701 gccgtctgtg gaacggcgac ctcgtcttcg acgtctacgc gcccggcgag accaaggtcg
    32761 gcgggctgcc cgtgccggaa acgggtttca tcaaggagac catccgcttc accaaagtcg
    32821 acggcgtctg gaagatcgcc gaggcccagc acttcggcgc ctgagcgcgc tcaaggcagc
    32881 acgggcagac cgtcgtcgtc gatcggccag agggggttga aggctacctc ccaggcatgc
    32941 ccgtcggggt cggcgaagta cccggagtag ccgccccaga agcggtcctg ggccggccgc
    33001 aacagcgtcc cgccggcccc gaccgccgcg tccaggcagt cgtcgacgag ctcgcgcgac
    33061 tcgacgttca tcgccagcgt cgcgcggatc gagccgggct ccccgggtgt ctgcccggcc
    33121 tcctcggcga tgctctcgag ccggcagagc gcgagccgcg agccggcgtt gcggaagaac
    33181 gcgacctgtt cctccggttc gccgctcagc ggccaaccga gcgccaggta gaacgtccga
    33241 gcccgtggga cgtctgcgac tcccagggtg atcacgctga tccgtgcctc catcgccggc
    33301 ctcctctccc gacgccctgt ccaagactgc cgctggggtt ttcaccatct cttacgggag
    33361 tcgccgcagt gtgcatggtt atactcaagt gaacgcagcg cgcacgcgga cgctctccgc
    33421 ctccacgacc tcccgaggag accatgaagc cacgcatcat ccgagagaac gtgtccctca
    33481 tcggcgccgt cgactgggac cgccgcctgt tcgacgcgct catccctctg cccgatggca
    33541 ccagctacaa cgcctacttc atcgccggca gcgagaagaa cgcgctcctc gacagcgtcg
    33601 atgcgccaaa gtggcccgtg ctctccgctc agctcgacca gctccccacg cccgactacc
    33661 tcgtcatcca ccacgtcgag caggaccact ccggctcgac accgatgctc ttggagcgct
    33721 ttcccgagct caccatcgtc tgcaacgaga aggcgaaggg catgctcatc gatcacctgc
    33781 acgtcgacga tgcccgcttc cacgtggtcg ccgacggcga cgagctgtct ctcggcgaca
    33841 agacgctgaa gttcctgttc acgccctggg tgcactggcc cgagaccatg agcacctggc
    33901 tcgaggaggg cggggtgctc ttcagctgtg acttcttcgg ctcgcactac gcgacgagcg
    33961 acgtgttcgc agacaagcac gaggtgtacg acggcgccaa gcggtactac gcggagatca
    34021 tgatgccgtt cggcgcccag gtgctgaaga acgtcgagaa ggtgacgcag tacccgatcg
    34081 agatcatcgc gcccagccac ggccccgtct acgacgaccc cgccttcatc atcgaggcct
    34141 accgcgactg ggccggcggc gagccgcaga acatcgtctg tctgccctac gtgacgatgc
    34201 acgacagcac ccggctgatg gtcgaccacc tcacggcggc gctcgccgat cgcggcatcg
    34261 gcgtcgagcg cttcgacctc accacgctcg acgccggcga gctcgccagc tcgctggtcc
    34321 acgccggcac catcgtcatc ggcacgccca ctgtgctcac cgggccgcac ccgctcgcca
    34381 tgtacgcggc cgcgctcgcc aacgcgctgc gcccgcgggc caagttcgcg accgtcatcg
    34441 ggtcctacgg gtgggccggc aaggcggtcg agacgctggt gggcatgatg cccaagctca
    34501 agctcgagtt gctcgagccg gtgctcgcca aaggcatgcc gcgagaggcg gactacgccg
    34561 cgctggacgc cctggcggac accatcgccg agaagcacca ggacctcgcg ccgcgcaact
    34621 tcgccgacct cagcgtctgc cgcactgacg gctgaggtca gaggcctcat cgcgggcgtg
    34681 agtggatcgt gcgcgcccgt acgtgtctgc cggagtccgt gaagtagagt acggccatgc
    34741 tcaccgtggc cttcgggctc gcatcggcgc tcggctacgc ggtccacgac tacctgctcg
    34801 tgcgcatcgt gcgctccgtg tcggtgtgga cggcgctcat gtgggtcatg ctcgtcggtc
    34861 tcgccgtgtt gctgccgctg gcgctcgtcg tcgacggtct gccgagcgga gacgagcagt
    34921 ggcgcgccgc cgggttcgcg gcggccagcg gcgtgctcga gttcctcggc ctgggcgcgc
    34981 tcctcaaagg gctgtccacc ggcaatctct ccgtcgtcac gccgctcggc tcgctggccg
    35041 gcggattcgc cgctggggcg gccatcctgc tcggcgaggc gctgccgccg atcgcctggg
    35101 tcgggctgcc gctggcggtg gccggagggc tgatggcgtc ggtcgagcgg gcgcccgagg
    35161 ccgctctggg cgcgcgccgc cggacgcagg ccacggccgg cgcgggctgg gcgctgctct
    35221 ccgcggccct gttcgccggc acctttctct tcttcgccga ggctacggcg ctgccgccgt
    35281 tctctctcgc cgcgatcggg agggcgggca ccgtggtcat cgtcgtcccg gtggcgctgc
    35341 tcacgggggg gctggtcctg ccgcgcgagt tccgtcgccg ggcgaccgtc gccggggtcg
    35401 tggatgcggg ggccttcgtg gcgctcgccg cagctatcag cctggggccg gtcgccatcg
    35461 cctcggtgtc ggtcgcacag gcgggcacgt gggcggtcat catcgggctg gtcgtgctgc
    35521 gcgagaggct gggccgcgta caactggtgg gcgtcgcgct cacgtgcatc gcgatcacga
    35581 tgctggccgc ggcgggactc gagtagtccg gaggcgagcg ccctccgcac gccggacgcg
    35641 ctcagccgcc gcgctctcgc aggtacgcct cggcgagctc gcgcagcgtg gtccgcccga
    35701 acggccggcc gtgtcccggg tagacgacgt cgacgtcgat ccggcgcagg acctccatcg
    35761 tcgccaggta ggtctcgacg tcggactccg gcagcgtgtc gatgagcccg ccgtcgtaga
    35821 cggcgtcacc ggagacgagc gcacgctcct cgtggtcgat gagcccgatg ctgccgggcg
    35881 tgtggccggg caggtcgacg atggtgaacc gccggtcgcc gaggtcgatc tcgtcgccgc
    35941 ctcgcacgaa gtgggtggga gcggcgcgct cgggccggaa cgtgaacgga tcgaacccgg
    36001 gggagggaag ggcgtcgacg aggaccggcg gcggcacgaa cccgctctcg gcgagctggg
    36061 ccaggatctc ctcggaccac agcgccggcg ccagcggtgc gtcgttgtgc ggcgtcgtcg
    36121 cgagctcctg ctcggcgggg tgcagcagac gccgctcgaa ctcgtggaag ccgccgatgt
    36181 ggtcgatgtg cgcgtgcgtg accacgcaga cgatctcgcg ggggcgtcct ccgcgcgcga
    36241 ggcgtgcgag gtgccggcgc agcggggcca cgccgttgtc ggtgtcgatg aggaggtcgc
    36301 ggtctcgccc gcggaagtac cacaggctcg cgcggagcag atcgtccacg tacggctcgg
    36361 tgagcgccgc cgtgcggccc gtcacccagg tgaggctgaa ccagtcggcg gcgaccggga
    36421 gctgtggcgc gccgtccatg ccgtcatcct tcccgccggg agtggaccgg cgcaaccgat
    36481 cgggcgcgac cggcgggcgg aagcactggg gacccgcacc cgtggtcgtg ctcgccgtgc
    36541 gccggatgta ctcgggtaga ctggccgcgt gctcaccgtc ctgacaggcc tcctgaccgc
    36601 gctctcgtac gcgaccagcg acatgttctc gcagcgggtc acgcggcaga cgcgggcgct
    36661 gacgcagatg ctctgggtgc tcgcgaccgg cgtgatcatc atccttccgg tcgcgctcct
    36721 catcgagggg atcccggcgg ccggcgagtg ggggggcgcc ggcctggcgg cgctcgccgg
    36781 cgccctgtac ttcttcgccc tcttctgcct gctgcgcggg ctgaacgtgg gagacctggg
    36841 cctggtctcg gcgctggtct cgctgcaggg cgcgtacgtg gctgtcgcgg tcgtgctgct
    36901 gggcacgccg gtgaccctgc cgctcgcggt cgccctggga ctgtgcgtgt tcggcgccat
    36961 cctcacctcc atcgagggcc gcgccggctc gaccaagggc gcgccctggg ccatcgtcgc
    37021 cggcatcctc ttcgcctgca tcatgctctg ctacaactac gcggacatcg gctggctctc
    37081 gcagacggcc atctcgcgga cggtgtcgct tgcggtgacg ctgccggtcg ccctcctcag
    37141 cggcagcgtg gcggtaccca aagcgttgcg cacgcgtgcg gtcggcgccg gcgtgctcga
    37201 gctcgcgggc ctctcgctcc tcacgatcac cttcgcgctc gggccgccca cggtcgccgg
    37261 cgtgaccacc acccagttcg gcaccttcgc ggtgatcctg ggcttcgtgc tgctccacga
    37321 gcgcccgaag cccaaccagt gggtgggcat cgcctgcacg atcgccggcg tctcgctcat
    37381 cgcggcgatc gtctgacgcg tgcgacgttc acccgcgcgc gtccttccgg tagactgggc
    37441 cgaccttccg gggacccggc cccacacccg tgggtagccg cgccgagccc ggccgttcgt
    37501 caaggagatc cgagccgatg gcagccaaca tcatcgacga tagtcatctg aagaagaccc
    37561 aggagatgtt cgccaagtgc caggaggtcg tctcgggcgg cgagtcaagc tatgcccgcc
    37621 tcatcggcac ccgcccgatc gtcatggagc gcggagaggg cgcctacttc tacgacgtgg
    37681 acggcaacgc ctacgtggac tggtgcatcg gctacggccc catgatcttc ggccatcgcc
    37741 ccaagcccat cctcgacgcg atcatcgctc agatctcgca gggcggcatg ctctacacgt
    37801 tcccgcacga actggactac gaggtcgggc gcaagatcgt ccaggcggtg cccggcatcg
    37861 accaggtgcg gttcgccaac tcgggcagcg aggcgaccca ggcggccatc cgcctcgcgc
    37921 gcgcctacac cggcaaggac aagatcatga agtgggaggg gagctacaac ggcttcctgg
    37981 actgccacgc gttctcgcac gtgcctgcgc tcgaggcggc gggccccgag aagttcccgc
    38041 gcacgctgcc gagccaggcc ggcattccca aggcggtgga gggcaccgtc gtcatcgcgc
    38101 ccttcaacga catcgacgcc gtcgaggtgc tcatcaagcg caaccgcgac gagctcgcct
    38161 gcgtgctcgc cgagcccatc ctcggcgacg ccggcatcat cccgccggtg ccggggttcc
    38221 tcgagggcct gcgccgcatc tgcgacgaga acgacgtggt gctcatcttc gacgagatca
    38281 tcaccgggtt ccgcgtctct ctgggcggcg cgcaggagct ctacggcgtc atcccggaca
    38341 tcacctgcat cgccaaggcg gtcggcggcg gcacgccggg cgccgccgcg ttcggcggca
    38401 agaaggagtt catggacctc gaggccgagg gcgtggtgct ccacggcggc acctacagcg
    38461 gcaacccgat gacgctcgcc ggcatgaacg ccgcgctcga catcctgatc aacgaccgcg
    38521 agcgcgtcta cgggcacctc aacacgatcg ccaacgcgat ggtcggcggc atgaggagca
    38581 tcttcgcgga gaacggcgtg cccgcgtaca tcagccaggt cggccccatg tggcaggtgt
    38641 tcttcggcca cgaggagccg gtgacgaagt accgtcaggc gcgcgccagc gacacgcgct
    38701 tcttcggcat gctgcagacc gagtgccaga tgcgcggcgt gtacttccac aacttcaact
    38761 tcgagcgctt cttcgcgagc acggcgcaca ccaagaccga ggtcgaccgc agcctcgagg
    38821 tgatcgccga ggcgaccaag atcgtcaaga agcggctcgg cgacctgccc gccggggtct
    38881 gagccgccga cgggacaggg ctcagtccgc cggcgcgtag cggcggaagc cctgctcgtc
    38941 gaagtgagga tccaggccga ggtactcgtc tatctggcgt gcgcgcatca ccgcgaagct
    39001 gacgcaatcg accaggctca gccgtcgcct gcccgcagtc aggagagccc cgacgcccgc
    39061 gccgtgctcc gcgtcggtga cccacagcgg ctcgatcatg ggcagcatct cgccggtcag
    39121 caagcgcacc gcctcgagac caagtcgcgc ctgcaccagc gtgatcgcct ccagcagcac
    39181 gtagttcgtg gtcacgaagc cggcgtcgtc gtccactccg tgatcccacg ccggcctcat
    39241 gagaccgtgg ccggcgtcct cggcgtcgag cagcgcaacg agagcgcttg tgtcgacgaa
    39301 gacgctcacc actcgatgga atcgacgaag tagtcgtcgt gccgagcagc cacgtccccc
    39361 aaccccgacc ggaagcggcc gcacgccgct ttggcccggc gcttgcgctc ctcgaggctc
    39421 acggcggact tgcgctggat cagacgctcg tccacggcct cacggacgac ggacgaaatg
    39481 gagacctgac gctgtgcggc ttcgtctctc agccgtcgtg cctcgtcttc cgtgaactgg
    39541 atctgcatcc tgaccatgcg gtgcctcctc accactgatg gcatctctgc cagtatgatg
    39601 gtatctacgg gcggtgtcca gaggacgatc gcgagctggc gggccggcta cttcagcgca
    39661 ggtggagcac ccagagcgtc tggtacacga ccacggcggc gagcacgccg gccagcagcc
    39721 ccgccgcgac ggtcgcgatg cgataggccc gcagccgcca gagcagcagc gcgccgacgg
    39781 tcacgatgcc gagcttgaag gcgagggcct gcaccgcgcc ggcgcgcagg aagaagtcca
    39841 tgatgccgtt ggactcggtg gcgaagccga gccacatcga atagcgcgtc agcgccagat
    39901 cggcggcgtt gagcagccac agctcggcga ggagcaggag aagcaggcgg tcacgcgact
    39961 cgctggggcg cggcagcgtg cggcggacgc ggaacgcagg ggtcattgcg ggagcctcgt
    40021 cacgacgacc gcgtgggtcg agccgcccat cctctggatc caggcgaccg tcgcgccact
    40081 gacggcgacc tcggtgacgg cgccgtccgc ctccgcgacg acgaaggcgc cggtctcccc
    40141 ggggcggcgt cccatcacgc ggtcgccgga cgccgtctgc tccgcccaga ccaccgtgcg
    40201 gccgtcgtag gacgggccgg ccaggccgga gacgccggag gccaccgtgg tggtggggcc
    40261 gccgtcgacg gcgaccgcgg cgacgacgcc ggaaccatct gcctcgccct ggccccacac
    40321 gagcgtgcgg ccggccaggg cgatggcggt cagccttgcg cccgtgagcg aggcggacac
    40381 cggccaggtc gtgccggtga ccagatctcc ggccacgaag ggccccgtcc agccggtcgc
    40441 ccagacgacg aggctgccgg agacgaccgg ggcgccgtcc ccctgggcga cctcgaacgc
    40501 ttcaccgctg ccgacccgcg ccccgttgat cgtcggcgcg ccgatggtgg gtgcggaaca
    40561 ccagaccgcg acgtcgcccg agatggccgg gtaggagcgc acagagccga cctcggccag
    40621 cgtccagcgc ctgcccgact ccgtgtcgta ggcgacggcc tggacggcga ggctcgcctc
    40681 gcgctccgcc tcgaaccaca ccgcgtaccg ctcgcctacc gccgggtccc acgtggtgcg
    40741 catccccggg ccggggccga ggaggcgcag gtcgcccgag gcgaggtcga ggtactcgat
    40801 ggaggcgccg ttctgccaga gcagccggtc gccgctcagg gcgaggccgc tgaacgcgag
    40861 ctggtggtgt gcggtgaccc tccagccgcc cgccgtccgc tcgacggcgg gcggctggat
    40921 ggaggccgac cgctcgaagc ggtaccacgc cgcgcccagc gcggacccgg cggcgaggac
    40981 caccagcgag aggaccaggg cgcgcaacca ggacggccgg cgcggcagcc tctcgccggc
    41041 gcccggcgcg gcggtcgcgc ccaccaggac cggcggagcc gccgcggacg acgccggggt
    41101 cgcggccggc cggccggtca gatcgtccgg cacaggtgca gcaggtccgc gtagacgccg
    41161 taggccgtct gcggcaccag tgcgtggcgc tcgctgacca ggacgtcggc catgaggtcg
    41221 gtgtgcagca gcgcccccag cgactcgccg ctgacggcgg cgagcgggtg ctcgatgtcg
    41281 agctcctcgg cgcgcagccg ggcgcggacg gggccgctcc cgtgcggcgc gtcggggccg
    41341 gccggcgaag cctcgccggc ttctccctcc cgccagacgc tgcagaccag ccgcaggcgc
    41401 cggccggctc tccgcgcgac gacgatccgc tccagcggca cgtcgcggag gctctcgcgc
    41461 tccacgtccg ccggcgtcag gcacgcgtgc atggcgacgt tggcgagcgc ggcggccttg
    41521 ctggcggcgt cccagccgtc gatgtcgttg ctcgggtccg cctccgcgta gccctcggcc
    41581 tgcgtctgcg ccagcgcgtc gtcgaaggtc ctgcctgcgc ccatcgcgtc gatgatgaag
    41641 ttggtcgtcc cgttgaacac cgcctcgaag ccgagcacgg tgcagtcggg gagtgcgtac
    41701 tccaggaggc tgaagacggg gaggccgtcc atgacggtgc tctcgaagcg gatgcggcgg
    41761 ccgctcgcgg ccgccgcatc cgagacctcc ggccagctcc aggcgatggg tcccttgttg
    41821 gcggtgacga cgtccatgcc caggcggaag gcctcgagga cgtggtccgt ggccgccggc
    41881 cggtacgtcg cggcgccggc gccgacgccg gagaggtccc ccatcaccgt gagctccacg
    41941 aggacgtccg cctcggaggc ggcgagcagt ccaggggccg gccggggcgc ctgcggcggg
    42001 gccggcccgt cgccgatcgc cgcgagcgcc tccgccggcg tcatgccgtc gggatcgagc
    42061 aggctgccgc ggcgcgtgcc cacggcgctg atcagcacgc ggagtccgtg ccggccggcg
    42121 agcacccgct cctgggcggc cagcagctcg cagagagctc tggcgacgct gccgaacccg
    42181 agcaggcaga gcttgagcgt gcggggcgtg ccccgagccg gctccgcctc gaagcctgac
    42241 agcgagatgc ggacgtcgcg gggggcgccg ggcggcgcgg ctgagccggc gctcaccgta
    42301 cggagcggga gaggtcgccg accgcggcca ccaggcgctc gagctcgccc tccgtggtgt
    42361 acggggcgag ccccacgcgc accagaccgc cgcgggggtg gagcccgagc agctccacga
    42421 gccggtgggc gtagaagtcg ccgtcccagg tgaagaggcc gcgcgcgccg agggcccggc
    42481 agacctcgcc ggcctggaac ccgtccacgg tgaacgagac ggtggacgtg cgcgggctgc
    42541 cctccggcgg cccgtagagc gtgagtccgg ggatgccggc gagctgctcg cgcaggcgct
    42601 tcgcgagcgg ctgctcgtag gcctcgccgg ccagcatgcc ggcgacgacc gccctgcgcc
    42661 ggccctgcag gccggcgggg aggcggtcct ccaccatcgc cacgtgccgc tcgccgaggt
    42721 cggcgatgaa gtcgatcgcc gccg
//
LOCUS       DASUMX010000106         5117 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_460248, whole genome shotgun sequence.
ACCESSION   DASUMX010000106 DASUMX010000000
VERSION     DASUMX010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 5117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5117
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460248"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VFZ86_05265"
     CDS             complement(<1..215)
                     /locus_tag="VFZ86_05265"
                     /inference="COORDINATES: protein motif:HMM:NF039728.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6325 family protein"
                     /protein_id="HEX5641736.1"
                     /translation="MAMGPVQVLVVGFGPDADFKGEALRELKRLTEQDIVRVIDLLVV
                     HKEEDGSIDKVEISDEHELAKLGALAGA"
     gene            complement(255..533)
                     /locus_tag="VFZ86_05270"
     CDS             complement(255..533)
                     /locus_tag="VFZ86_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEX5641737.1"
                     /translation="MRRRPLMAAAVVGGTAYHIGKKGQQSRDQQAQIDEMQAQQDQQQ
                     YEQAPPQAAPAPEEDPMAKLTQLKSLLDSGVLTQAEFDFQKTKILQSM"
     gene            complement(671..2170)
                     /gene="cls"
                     /locus_tag="VFZ86_05275"
     CDS             complement(671..2170)
                     /gene="cls"
                     /locus_tag="VFZ86_05275"
                     /inference="COORDINATES: protein motif:HMM:TIGR04265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEX5641738.1"
                     /translation="MIASSAGVLMGSFFTQNLFSWGFWVVDIILLLAIAGVVIVLATD
                     DRDPSTVLAWLFVVLLLPVLGLVAYFFIGRNFRRDSPRRRKIMAQMDAVAAESLAPVT
                     AANTEFTEAARADLAGTPGARIEAVGLTEGGVAPLPADTVDIYTAGSQKFPALLEEMA
                     TAERYIHLMYLIWEQDELTAKVTKVLLDRLQAGVKVHILYDWLSCISYKKDELKQLEA
                     AGAVVAPCYKRLPQINYRNHMKMVIIDGKSVYSGGMNMGQEYIDGGPRFDVWRDTSFR
                     LSGPVVAPYLMLFASTWLYNGGTEDLATDFMPPAAEHHRHEGTPVQVLHSSVQTTFKT
                     NRDVFITALTNARERIWIQSPYFVPDEPLMTAMCTAAASGIDVRFMMTGVPDKKIPFN
                     AAMAYYPQLLRAGVKVYQYKAGFLHAKTVTVDDQLSIIGTCNWDIRSLILHDEVVAVF
                     YDGGVATRYAEQYERDIEDCELIATDFLTSLTRGQRFRNSVARLFSRLL"
     gene            complement(2234..2878)
                     /locus_tag="VFZ86_05280"
     CDS             complement(2234..2878)
                     /locus_tag="VFZ86_05280"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEX5641739.1"
                     /translation="MDQLLDHILHFDTYLTQAVEALGLWAYAAIFATIFCETGLVVAP
                     FLPGESLLLASGTLAGADVLEIVVLWPLLVGAAFLGDVVNYLIGRWTGRHLLSKPRKH
                     LKPEYVEQAHAFYEEHGGLAIVLCRFLPIVRTLVPFVAGMARMEFHRYVAFAALASAI
                     WVTIFLGAGYLFGNIQWVQDYLGVALAIAVAASAIPGFIIWGMHHLRRQKTEGD"
     gene            complement(2898..4094)
                     /locus_tag="VFZ86_05285"
     CDS             complement(2898..4094)
                     /locus_tag="VFZ86_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015054378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641740.1"
                     /translation="MGVTEWTIVVLVIGGRLALPLLIPYFPVPALLACLILDSIDQSI
                     FQQFPAIPLDGYQSYDKALDVYYLSVAYLSTLRNWTNQGAFRMSQFLYYYRMVGVVAF
                     ELTQNRTILFIFPNTFEYFFLFVELVRLGWNTDRMGKWTVILSAAGIWIFIKLPQEWW
                     IHIAQLDMTDFIKETLFGVAATDSWATAIANRPWVLVAAIAFFAALAAIVYWIITRKA
                     PKFDHKAHIAADPLPPLLRGAEPYRKVRAETRIFGWALREKVALTAIVCVIFGLMLTG
                     GEASTLRIVVGVAIFTVINAAVSHVMAIRGRSWRRLAGELVTMLVVNLVIVLVLEALD
                     RYLGLTSARTPFGMTLFFSYLLTLVIVLYDRYRTVYKARRLYRGEEKAAQTAAGTPSG
                     PSAAPA"
     gene            complement(4107..4391)
                     /locus_tag="VFZ86_05290"
     CDS             complement(4107..4391)
                     /locus_tag="VFZ86_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641741.1"
                     /translation="MSVAIIAIIAVLLVLGIGYLIFVLMRRSQTVVPQVTDNDAASRD
                     HVVEVDDQGREIMASQEAPPAPRDTAGFESLLQDEIHDLGMRQPPADDES"
     gene            4645..>5117
                     /locus_tag="VFZ86_05295"
     CDS             4645..>5117
                     /locus_tag="VFZ86_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009377328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HEX5641742.1"
                     /translation="MSEMMVLGFENEMEADRFGLKLAELQKDMIVQLSDAAEVVRDPD
                     GKPHVKHGHGLVGAGAMGGAFWGMLFGLLFFVPFLGMAIGAGMGALFGKLGKTGIDKE
                     VLQQMGDAVPPGKAGWFLVIQELTEDKFLAAIEGTNATLVRSNLSAEQEEQLKHA"
BASE COUNT          835 a         1641 c         1799 g          842 t
ORIGIN      
        1 gccccggcca gcgcgccgag cttggcgagc tcgtgctcgt cggagatctc gaccttgtcg
       61 atgctcccgt cctcctcctt gtgcacgacg agcaggtcga tcacgcgcac gatgtcctgc
      121 tcggtcagcc gcttgagctc cctgagcgcc tcgcccttga agtcggcgtc cgggccgaag
      181 cccacgacga ggacctgtac gggtcccatt gccatgccag tctccttctc cgtgatcagc
      241 cgcgtcgcga ccggctacat gctctgcagg atcttggtct tctggaagtc gaactcggcc
      301 tgggtgagca cgccggagtc gagcaggctc ttgagctgcg tgagcttggc catggggtct
      361 tcttctgggg ccggcgcggc ctgcggcggc gcctgctcgt actgctgctg gtcctgctgc
      421 gcctgcattt cgtcgatctg cgcttgctgg tcgcggctct gctggccctt cttgccgatg
      481 tggtaggcgg taccgccgac gacggcggcg gccatcagcg gccttctcct catgcgcatg
      541 ctgatcctcc ctctctgttg accggtccac gagcgggccg gcgccatgct tggccgaatg
      601 gtaacagcgt ctggaggctg cgcaacggct gcggcctccc gcccgccttc attcccgctg
      661 ccgcgccgtt ctagagcagc cgggagaaca ggcgcgccac ggagttgcgg aaccgctgtc
      721 ccctcgtcag cgatgtcagg aagtccgtgg cgatcagctc gcagtcctcg atgtcgcgtt
      781 cgtactgctc cgcgtatcgc gtggcgaccc cgccgtcgta gaagacggcg acgacctcgt
      841 cgtgcaggat gaggctgcgg atgtcccagt tgcaggtgcc gatgatggag agctggtcgt
      901 cgacggtcac cgtcttggcg tgcaggaacc ccgccttgta ctggtagacc ttgacgccgg
      961 cgcgcagcag ctggggatag taggccatcg ccgcgttgaa cgggatcttc ttgtccggca
     1021 cgccggtcat catgaagcgc acgtcgatgc cgctcgccgc cgcggtgcac atcgcggtca
     1081 tgagcggctc gtccggcacg aagtagggag actggatcca gatgcgctcg cgcgcgttgg
     1141 tgagcgccgt gatgaacacg tcacggttgg tcttgaacgt cgtctgcacg ctcgagtgga
     1201 gcacctggac cggtgtgccc tcgtgccggt ggtgctccgc cgccggcggc atgaagtcgg
     1261 tcgccaggtc ctccgtgccg ccgttgtaga gccaggttga ggcgaagagc atgaggtagg
     1321 gcgcgaccac cggtccgctg aggcggaacg aggtgtcgcg ccacacatcg aagcgcgggc
     1381 cgccgtcgat gtactcctgg cccatgttca tgccgccgct gtacacggac ttgccgtcga
     1441 tgatcaccat cttcatgtgg ttgcggtagt tgatctgcgg caggcgcttg tagcaggggg
     1501 cgacgacggc tccggcggcc tcgagctgct tgagctcgtc cttcttgtag ctgatgcagc
     1561 tgagccagtc gtagaggatg tggaccttga cgccggcctg gaggcggtcg agcaggacct
     1621 tggtgacctt ggcggtgagc tcgtcctgct cccagatcag gtacatgagg tggatgtagc
     1681 gctcggccgt cgccatctcc tcgagcaagg cggggaactt ctgcgagccg gccgtgtaga
     1741 tgtccacggt gtcggccggc agaggcgcca cgccgccctc ggtgaggccg acggcctcga
     1801 tgcgcgctcc cggggtgccg gcgaggtccg cgcgggccgc ctcggtgaac tccgtgttgg
     1861 cggccgtcac gggggctaac gactccgcgg ccacggcgtc catctgcgcc atgatcttgc
     1921 ggcgccgcgg tgagtcgcgg cggaagttcc tgccgatgaa gaagtaggcg acgaggccca
     1981 gcaccggcag cagcagcacg acgaacagcc acgcgagcac cgtgctcggg tcgcggtcgt
     2041 cggtggcgag cacgatgacg acgccggcga tcgccaggag caggatgatg tcgaccaccc
     2101 agaagcccca gctgaagagg ttctgcgtga agaaggagcc catcaggacc ccggcagagc
     2161 ttgcgatcat gacgtcctcc cgtagattcg ccggtggcgc attctcacac acacgagctg
     2221 cgttaccaag cggtcaatcc ccctcggtct tctggcgccg caggtggtgc atcccccaga
     2281 tgatgaagcc ggggatggcc gaggcagcga cggcgatcgc cagcgcgact ccgaggtagt
     2341 cctgcaccca ctggatgttg ccgaagaggt agccggcgcc gaggaagatg gtcacccaga
     2401 tcgcggaggc gagggcggcg aaggcgacgt agcggtggaa ctccatccgc gccatgccgg
     2461 ccacgaacgg caccagggtg cgcacgatcg gcaggaaccg gcagagcacg atggcgaggc
     2521 cgccgtgctc ctcgtagaac gcgtgcgcct gctctacgta ctcgggtttg agatgcttgc
     2581 gcggcttgct cagcaggtgg cggcctgtcc agcgcccgat gaggtagttg acgacgtcgc
     2641 cgaggaaggc cgcgccgacg agcagcggcc agaggacgac gatctcgagc acgtccgctc
     2701 ccgccagggt gccgctggcg agcagaaggg actcgcccgg caggaacggg gcgaccacca
     2761 gcccggtctc acagaagatg gtcgcgaaga tggccgcgta cgcccacagg ccgagtgcct
     2821 cgaccgcctg ggtcaggtag gtgtcgaagt ggaggatgtg gtcgaggagt tggtccacga
     2881 ggggacctgg cccgggatca ggcgggcgcc gcggacggtc ccgacggcgt gcctgccgcg
     2941 gtctgcgccg ccttctcctc gccgcggtac aggcgccgcg ccttgtacac cgtgcggtag
     3001 cggtcgtaca gcacgatgac cagcgtcagc agatacgaga agaacagcgt catgccgaac
     3061 ggcgtgcgcg cggacgtgag gcccaggtag cggtcgagcg cctccaggac gaggacgatg
     3121 acgaggttga cgaccagcat cgtcacgagc tcgccggcga gccgccgcca gctgcggccg
     3181 cggatcgcca tgacgtggct cacggcagcg ttgatcaccg tgaagatcgc cacgccgacg
     3241 acgatgcgca gcgtgctcgc ctcgccgcct gtgagcatga ggccgaagat cacgcagacg
     3301 atggccgtga gcgcgacctt ctcccggagc gcccagccga agatcctggt ctcggcgcgg
     3361 accttgcggt acggctccgc gccgcgcagc agcggcggca gcgggtcggc ggcgatgtgc
     3421 gccttgtggt cgaacttcgg cgccttgcgc gtgatgatcc agtagacgat ggcggcgagg
     3481 gcggcgaaga acgcgatcgc cgcgacgagc acccacggcc ggttggcgat ggcggtcgcc
     3541 cagctgtccg tcgccgcgac gccgaacagc gtctccttga tgaagtccgt catgtcgagc
     3601 tgggcgatgt ggatccacca ctcctgcggc agcttgatga agatccagat gcccgccgcc
     3661 gacaggatca ccgtccactt gcccatgcgg tcggtgttcc agcccaggcg cacgagctcc
     3721 acgaagagga agaagtactc gaacgtgttc gggaagatga acaggatcgt ccggttctgc
     3781 gtgagctcga acgccacgac gccgaccatg cggtagtagt acaggaactg gctcatccgg
     3841 aaggcgccct ggttggtcca gttgcgcagc gtggagaggt aggcgaccga gaggtagtac
     3901 acgtcgagcg ccttgtcgta ggactggtag ccgtccagcg ggatcgccgg gaactgctgg
     3961 aagatgctct ggtcgatcga gtcgaggatg aggcacgcca gcagggcagg cacggggaag
     4021 tacgggatga gcagcggcag ggccaggcgc ccgccgatga cgagcacgac gatggtccac
     4081 tcggtgacgc ccacgtccgc ctcccgtcag ctctcgtcgt cggccggcgg ctgccgcatg
     4141 ccgaggtcgt ggatctcgtc ctgcaggagg ctctcgaacc cggcggtgtc gcgcggcgcc
     4201 ggcggcgcct cctgcgaggc catgatctcg cgcccctggt cgtcgacctc gacgacgtga
     4261 tcgcggctcg ccgcgtcgtt gtccgtgacc tgcggcacga ccgtctggct cctgcgcatc
     4321 agcacgaaga tcaggtagcc gatcccgagg acgagcagga cggcgatgat ggcgatgatg
     4381 gcgacgctca ctgggacctc ccggctcgcg gacaacggta gcctagcccg ccttctgccc
     4441 gcgtgtccat gcgcgggcag ggtggagcgt gccgttccct ggccgccgct ctcccggttc
     4501 ggcagggccg tctcacgggc aggtcacccg ccggtcacct cgaccgggcc cagtgacaaa
     4561 gccggcttcg cgccgggggt tagtgaagct cgacaagtac ggtaggcttg gcggcggcaa
     4621 gcagtgaacg aagaaggaga gtctatgagc gaaatgatgg tcctcggctt cgagaacgag
     4681 atggaggccg acagattcgg cctcaaactc gcggagctgc agaaggacat gatcgtgcag
     4741 ctgagcgacg ccgccgaggt agtgcgtgac ccggacggca agccgcacgt caagcacggc
     4801 cacggcctcg tgggcgcggg cgccatgggc ggcgcgttct ggggcatgct gttcggcctg
     4861 ctcttcttcg tgccgttcct cggcatggcg atcggcgccg gcatgggcgc gctgttcggc
     4921 aagctgggca agaccggcat cgacaaggaa gtccttcagc agatgggcga cgcggtgccg
     4981 ccgggcaagg ccggctggtt cctcgtcatc caggagctga ccgaggacaa gttcctcgcc
     5041 gctatcgagg gcacgaacgc cacactcgtg cgttccaacc tcagcgccga gcaggaagag
     5101 cagctcaagc acgcctt
//
LOCUS       DASUMX010000107         9317 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1754390, whole genome shotgun sequence.
ACCESSION   DASUMX010000107 DASUMX010000000
VERSION     DASUMX010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 9317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9317
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1754390"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="VFZ86_05300"
     CDS             complement(<1..281)
                     /locus_tag="VFZ86_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5641743.1"
                     /translation="MGLYDGKTALVVGVANRGSIAWGIAQALRDEGARLGFSYAAPAL
                     ERRVRPLAESAGAELIEECDVTDDAALDRLFERVGETFGELHVLVHAVAA"
     gene            complement(281..1537)
                     /gene="fabF"
                     /locus_tag="VFZ86_05305"
     CDS             complement(281..1537)
                     /gene="fabF"
                     /locus_tag="VFZ86_05305"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011959271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEX5641744.1"
                     /translation="MSDAPRRVVVTGLGIICPLGNDVESSWRRLVAGESGIATITRFD
                     PARVDSRVAGEVKGFDPADFIERRAARRMDAYSQYAVAAARQAVDDAGLDVAAEAEDI
                     GAVVASGGGGLKTFEDQQRALFERGPRAVSPFFATMMIPNMGAAHVSLHLGTKGPLQA
                     VTTACAAGAGGIGDAFEIIKRGAAVAMLAGGAEAPVNETGLAAFGAMRALSTRNDAPE
                     EASRPFDAGRDGFVIAEGAAILVLEELEHARARGARVLAEIVGYGMSSEAFHVALPDE
                     SGVPQARAMQAALREAGLSPDDVDYVNAHGTATPAGDIAETRALKIAFGARAAQVPVS
                     STKSMTGHPLGAAGAMEAVFCVRAITDGVVPPTINLTDPDPECDLDFVPGVARAASVR
                     VAMSNSFGFGGHDVSLVFSRYDEDGG"
     gene            complement(1534..2559)
                     /locus_tag="VFZ86_05310"
     CDS             complement(1534..2559)
                     /locus_tag="VFZ86_05310"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008738526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HEX5641745.1"
                     /translation="MARYHTVLGTRRVPDSLLRAIIRRRLRDKLEGLEAATGDDAAAL
                     RSYAAGLAAGPFAIHQDAANRQHYELPTAFFREFLGPRLKYSCCLWPDGVDDLAAAEE
                     AMLALTCERAGLEDGMRVLDLGCGWGSLSLFIAERFPACQVTAMSNSRTQGEHIAAAC
                     SAAGLGGVTPVTADIATYDPGRRFDRVLSVEMFEHVRNYRELFARLRGWLEPDGRAFV
                     HVFSHARFSYTFDADDPGDWMGSRFFSGGQMPAHDLFLQFDEHLVAEESWWLDGGHYA
                     RTLEEWLRRYDARAAAIKPLLATTYGESAADWWVDWRLFFLACAETFAYGGGREWGVS
                     HYRFAPR"
     gene            complement(2617..2754)
                     /locus_tag="VFZ86_05315"
     CDS             complement(2617..2754)
                     /locus_tag="VFZ86_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641746.1"
                     /translation="MTRYEHTTWSRLGDAARALGDAVATPRGAAPGRADRSAAMRRAQ
                     I"
     gene            complement(2760..2900)
                     /locus_tag="VFZ86_05320"
     CDS             complement(2760..2900)
                     /locus_tag="VFZ86_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641747.1"
                     /translation="MHIMYDFVALELARQRRDQVERAAERARLVREARPRKRRGHETQ
                     GR"
     gene            3062..3619
                     /locus_tag="VFZ86_05325"
     CDS             3062..3619
                     /locus_tag="VFZ86_05325"
                     /inference="COORDINATES: protein motif:HMM:NF018984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HEX5641748.1"
                     /translation="MATQSKEAPGDLAAVRAFVNTRDIDHGIEELDTPEALVAWLAGQ
                     GLLDEHATARPGDVRRAVLFREALRELLLSNNDGHQLPLDVGRKLNDVAARARLRLRM
                     DAGGLAHLQAEGDTVDAALGRLLVIVYRAMENDTWPRLKACREDTCRWAFYDHSKNRS
                     GHWCSMEVCGSRHKARVYRERRRSG"
     gene            3746..4342
                     /locus_tag="VFZ86_05330"
     CDS             3746..4342
                     /locus_tag="VFZ86_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641749.1"
                     /translation="MKKLMLLAGLTLLLVLVPAVPAFAYATTPADGAYVWPWDGGSWG
                     TVVGGEVTDEWNPPGTPIPAGTDVYIGGGWLAYTRGLVKTLPEYLLYKVAPTADPAHP
                     LMTYAQGKAYWSVMYQEAGLSEIYPAFNPKLGAELWTRDFWAPLPDAPGDYQLTLYEK
                     FRHTSTDLCGGYFEGQFRPVMFRAGVTQYPFVYSVVAG"
     gene            complement(4370..5011)
                     /gene="msrA"
                     /locus_tag="VFZ86_05335"
     CDS             complement(4370..5011)
                     /gene="msrA"
                     /locus_tag="VFZ86_05335"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEX5641750.1"
                     /translation="MRHSIAGLLAAVIVLALALAGCAAGSTDAGSPADTTEPATPTDA
                     GGDDVKTIYVAGGCFWGLEKYIGLVEGVVATEVGYANGASASATYGDGSGYAEAVKVV
                     YDPAVAPLPFLLDLFYDAIEPTSVNRQGNDVGVEYRTGIYYTDSADAAVIEKSLARLQ
                     TKYTEPIAVESGPLTTYTRAEDYHQDYLEKNPGGYCHIPQKLFDAASAARPLP"
     gene            complement(5045..5120)
                     /locus_tag="VFZ86_05340"
     tRNA            complement(5045..5120)
                     /locus_tag="VFZ86_05340"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5085..5087),aa:Lys,seq:ttt)
     gene            complement(5573..6766)
                     /locus_tag="VFZ86_05345"
     CDS             complement(5573..6766)
                     /locus_tag="VFZ86_05345"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5641751.1"
                     /translation="MRGPADLPALPRLSGGAITLRPFRDEDAATVAAVCDNEAAARWI
                     PGLPSPYTLDHGREWASDAARKWREERWANFAALDTATGRHIANCGMHLETEHARGEI
                     GYIVHPDWRRRGIGRACVALLTDWGFDDLGLERIEIRADVRNVASRRTAMAAGYRYEG
                     VLRRAMTVGDERVDDVLFSLVPGDPRPWQTGATEAGPAGLGWPRLTDGRLLVRPFDPD
                     DAPAVQAACDDADVAHWIFGLPAPYRLEDAEAFIADARHRLLLGERARLAVTDAAGEL
                     LGSVSLDCFAERQAAEIGYWVKREARRRGVALGAARLVVAWAFGELGVERLELLTYPG
                     NEASQALAARLGFARECLLRGFLAPEAGKSREGRAVPSPDGSLPPRDDQVQFVRLRSD
                     PAASA"
     gene            complement(6884..8458)
                     /locus_tag="VFZ86_05350"
     CDS             complement(6884..8458)
                     /locus_tag="VFZ86_05350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5641752.1"
                     /translation="MTSATRIYERRWQMLAVLSLSMMIIGLDNTIVNVALPTLQRQFT
                     ASASDLQWIVDAYLLVFAAALLTMGTLGDRYGRKRALQGGLILFGGSSAAVVFAGSVD
                     QLIVLRILMGLGAALIMPATLSIITNVFPREERGRAIGVWAGTAAIGIGLGPLAGGLL
                     LEVFSWSSVFLINVPVAIVALLLGIKLVPDSRDPAPGAFDLIGATLSAMALMALVWSV
                     IEAPSRGWTSTAVVAGLVAAALLTAAFIAWERRVASPMLNLTYFRNPRFSIGSLAIST
                     IFFSLMAAIFALTQFLQFALGYSALQAGATMVPLALGLMLSAINSSRLVAKFGTKRVV
                     AVGMLLLALTLSATVLWTPALSVWLVTSWLFVLGLAMGSVMAPATDAVMGSVPPAKAG
                     VASAMNDVTRQVGGALGVAIVGSLISTIYSSSIGDAAAGLPAALRGPVEASIGGADAV
                     AARLPAPAGAQLASAAADAFTTALGYGLLAAAVVAVIGAGIVAWRLPARHGAPEAGEP
                     PASLEPLGSLAAEAEA"
     gene            complement(8455..9066)
                     /locus_tag="VFZ86_05355"
     CDS             complement(8455..9066)
                     /locus_tag="VFZ86_05355"
                     /inference="COORDINATES: protein motif:HMM:NF028169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5641753.1"
                     /translation="MKVNEDAGAAKARAGRPRDPRLDEAVVAATLRLLAEEGYAQLTV
                     ERIAAQAGVGKASLYRRWPDKVSVVLEAVSRNPERPAVPDTGSLRGDALTYLQTLVRY
                     RTQHFDAISAISGEALSNQRFGDAFRAGMTEPVMAGMDTILQRAIARGEISPETDVAL
                     LSSVPPALLQMQRLLAGRHPDNDLVERIVDQFFSPDTDESELP"
BASE COUNT         1390 a         3263 c         3369 g         1295 t
ORIGIN      
        1 gcggcgacgg cgtgcaccag cacgtgcagc tcgccgaagg tctcgccgac gcgctcgaag
       61 aggcggtcga gcgccgcgtc gtcggtgacg tcgcactcct cgatcagctc ggcgccggcg
      121 ctctcggcga gcggccgcac gcggcgctcg agggcgggcg cggcgtacga gaagccgagc
      181 cgcgctcctt cgtcgcgcag cgcctgcgcg atgccccagg cgatcgaccc gcggttggcg
      241 acgccgacga cgagcgccgt cttgccgtcg tagaggccca tcagccgccg tcctcgtcgt
      301 agcggctgaa gaccagcgag acgtcgtgcc cgccgaagcc gaaggagttg gacatcgcga
      361 cccgcacgga cgcggcgcgc gcgacgcccg gcacgaagtc gaggtcgcac tcggggtccg
      421 ggtcggtcag gttgatcgtc ggcggcacga cgccgtcggt gatggcgcgc acgcagaaca
      481 ccgcctccat ggcgccggcg gcgccgaggg ggtgccccgt catcgacttc gtcgacgaga
      541 cgggcacctg cgcggcgcgc gcgccgaagg cgatcttgag ggcgcgcgtc tcggcgatgt
      601 cgccggccgg cgtcgccgtc ccgtgcgcgt tgacgtagtc gacgtcatcc ggcgacaggc
      661 cggcctcgcg cagggcggcc tgcatggcgc gcgcctgcgg cacgccgctc tcgtcgggga
      721 gcgcgacgtg gaaggcctcc gagctcatgc cgtagccgac gatctcggcg aggacgcggg
      781 cgccgcgcgc gcgggcgtgc tcgagctcct cgagcacgag gatcgcggcg ccctccgcga
      841 tcacgaagcc gtcgcggccg gcgtcgaagg gccgcgacgc ctcctccggc gcgtcgttgc
      901 gcgtgctgag cgcgcgcatc gccccgaagg cggcgaggcc cgtctcgttg acgggcgcct
      961 cggcgccgcc ggccagcatc gccacggcgg cgccgcgctt gatgatctcg aacgcgtcgc
     1021 cgatgccgcc ggcgccggcg gcgcacgcgg tggtcaccgc ctgcagcggt cccttggtgc
     1081 cgaggtgcag cgagacgtgc gccgcgccca tgttggggat catcatcgtc gcgaagaacg
     1141 ggctgacggc gcgcgggccg cgctcgaaga gcgcgcgctg ctggtcctcg aacgtcttga
     1201 gcccgccgcc gccgctggcg acgacggcgc cgatgtcctc ggcctccgcg gcgacgtcga
     1261 ggccggcgtc gtcgacggcc tggcgcgccg cagcgaccgc gtactgcgag taggcgtcca
     1321 tgcgccgcgc ggcgcgccgc tcgatgaagt ccgccgggtc gaagcccttg acctctccgg
     1381 cgacgcgcga gtcgacgcgc gccgggtcga accgggtgat cgtggcgatg ccgctctcgc
     1441 cggcgacgag ccggcgccag gagctctcca cgtcgttgcc gagggggcag atgatgccca
     1501 ggccggtgac gacgacgcgg cgcggtgcgt cgctcatcgc ggcgcgaagc ggtagtgaga
     1561 gacgccccat tcgcgcccgc cgccgtacgc gaacgtctct gcgcaggcga ggaagaacaa
     1621 ccgccagtcg acccaccagt ccgcggcgga ctcgccgtag gtggtggcca ggagcggctt
     1681 gatggccgcg gcgcgcgcgt cgtagcgccg cagccactcc tccagggtgc gcgcgtagtg
     1741 cccgccgtcc agccaccagc tctcctccgc gacgaggtgc tcgtcgaact ggaggaagag
     1801 gtcgtgcgcc ggcatctgcc cgccgctgaa gaagcgggac cccatccagt ctccgggatc
     1861 gtcggcgtcg aacgtgtacg agaagcgggc gtggctgaac acgtgcacga aggcgcggcc
     1921 gtcgggctcg agccagccgc gcagccgcgc gaacagctcg cggtagttgc gcacgtgctc
     1981 gaacatctcc accgagagga cacggtcgaa gcgccgcccc gggtcgtagg tcgcgatgtc
     2041 ggcggtgacg ggcgtgacgc caccaaggcc ggcggccgag caagccgcgg cgatgtgctc
     2101 gccctgggtg cgtgaattcg acatcgcggt gacctgacag gccgggaacc gctcggcgat
     2161 gaagagcgag agcgaccccc agccgcaacc caggtcgagg acgcgcatgc cgtcctcgag
     2221 gcccgcgcgt tcgcaggtca gcgccagcat cgcctcctcg gcggccgcca ggtcgtcgac
     2281 gccgtcgggc cacaggcagc agctgtactt gagcctgggc ccgaggaact cccggaagaa
     2341 ggccgtgggc agctcgtagt gctgccggtt ggcggcgtcc tgatggatgg cgaacggccc
     2401 ggcggccagc ccggcggcgt aactgcgcag cgccgccgcg tcgtcgcccg tggctgcttc
     2461 cagcccctcc agcttgtctc gcagccgccg ccggatgatc gcccggagca gggagtcggg
     2521 aacgcgtcgc gtccccagca cggtgtggta gcgcgccaac ggagctcctc tcagtctgcg
     2581 ggcgattgta cctgcgccgg cccgccggct ccacgctcag atctgcgctc gccgcatcgc
     2641 cgcgctgcgg tccgccctgc ccggcgccgc gccccgcggc gtggcgacgg cgtcgcccag
     2701 cgccctcgcc gcgtcgccca gcctgctcca cgttgtgtgc tcgtaccgcg tcatcgtcct
     2761 caccttccct gcgtctcgtg accgcgccgc ttccgcggcc tcgcctcgcg caccaggcgg
     2821 gcgcgctccg ccgcccgctc cacctgatcc cgtcgctgcc tcgccagttc cagagccacg
     2881 aagtcgtaca tgatgtgcac cttctccctc gtctctcgcc gtcggtggca cttgcgccac
     2941 tgtcgtcacc actgaaatca ttataccggt aatgcgtcac tagtaaaccc cttttggtgg
     3001 tataaagcaa gcggagccga gaccggctcc tgacgaggtg atctcgaggg aggtgcggac
     3061 gatggcgacg cagtcaaagg aggcgcccgg cgacctggcc gccgtgcgcg cgttcgtgaa
     3121 cacccgggac atcgaccacg ggatcgagga gttggacacc ccggaggcgc tggtcgcctg
     3181 gctggccggt cagggcttgc tcgacgagca cgcgacggcc cggccgggcg acgtgcgccg
     3241 cgccgtcctc ttccgcgagg cgctgcgcga gctcctgctg agcaacaacg acggccacca
     3301 gctccccctg gacgtcggtc ggaagctgaa cgacgtcgcc gcccgcgccc gcctgcgcct
     3361 gcgcatggac gcgggcgggc tggcccatct gcaggccgag ggcgacaccg tcgacgcggc
     3421 cctcggccgc ctgctcgtca tcgtctaccg ggcgatggag aacgacacct ggccgcggct
     3481 caaggcgtgc cgcgaggaca cctgccggtg ggccttctac gaccactcca agaaccgctc
     3541 cggccactgg tgctcgatgg aggtctgcgg cagccggcac aaggccaggg tgtaccgtga
     3601 gcggcgcagg agcggctgag cgtccccact ctcttcttct cgcggtggcg cggtcaccct
     3661 gatggggtga tgcgtgaggt cggccccggt gatggagtga gccgcaggga gtgcgccgca
     3721 ctccatcgag agacggaggg gagccatgaa gaagctgatg ctgctggccg gcctgacgct
     3781 gctgctggtg ctcgtcccgg cggtcccggc gttcgcctat gcgacgacac cagcggatgg
     3841 agcgtacgtc tggccttggg acggcggcag ctgggggacc gtcgtcggcg gagaggtgac
     3901 ggacgagtgg aacccgcccg gcacgccgat ccccgcagga acggacgtct acatcggcgg
     3961 gggctggctg gcgtacacgc gcggcctcgt caagacgctg ccggagtacc tgctctacaa
     4021 ggtcgcgccg acagccgatc ctgcgcaccc gctcatgacg tatgcgcaag ggaaggcgta
     4081 ctggagcgtc atgtaccagg aggcggggct ctcggagatc tacccggcgt tcaacccgaa
     4141 gctgggcgcc gagctgtgga cgagggactt ctgggcgccg ctgccggacg cgccgggtga
     4201 ctaccagctg acgctctacg agaagttccg gcacacctcg acagacctgt gcggcggcta
     4261 cttcgagggc cagttccgtc ctgtcatgtt cagggcggga gtgacgcagt acccgttcgt
     4321 ctactcagtg gtggccggct gagccggcac cagcgccgac aggtgcgaat cagggcagcg
     4381 gccgtgcggc agaagcggca tcgaagagct tctgcgggat atggcagtag ccgccggggt
     4441 tcttctcgag gtagtcctgg tggtagtcct cggcgcgcgt gtaggtggtg agcgggccac
     4501 tctcgacggc gatcggctcg gtgtacttcg tctgcagtcg tgccagggac ttctcgatca
     4561 cggcggcgtc ggcggaatcc gtgtagtaga tgccggtgcg gtactccacg ccgacgtcgt
     4621 tgccctgccg gttgaccgac gtgggctcga tggcgtcgta gaacaggtcg agcaggaacg
     4681 gcaggggggc cacggccggg tcgtagacga ccttcacggc ctccgcgtag ccgctgccgt
     4741 cgccataggt cgccgaggcg ctggcgccgt tcgcgtaccc gacctcggtg gcgacgacgc
     4801 cttccaccag gccgatgtac ttctcgaggc cccagaagca gccgccggcc acgtagatcg
     4861 tcttcacgtc atcgcctccg gcgtccgtcg gggtggccgg ctcggtggtg tcggcggggg
     4921 agccggcgtc cgttgaccct gcggcgcagc cggccaaggc gagcgcaagc acgatcactg
     4981 cggcgagcag ccctgcgatc gagtggcgca tacgagacac ggtgcacctc ttctcgcgtc
     5041 ggtgtggtgg gccgcgttgg gatcgaacca acgaccttgg gattaaaagt cccctgctct
     5101 accaactgag ctagcggccc gcggggagcg attgtaccac cggggtgccg ggcgcccgga
     5161 cgtggcgcag acggcgtccg cagccgacgt gaaccctaac ctttcggtag aggttgatgg
     5221 ctggtgcgac ggctgaggga ccgggccatg tcgccgaggc cgcaggcctg accttcgtgc
     5281 gaagcctgaa tcgactcgag gctacttgac gattgcctcc tgccggaaca tgggtaggat
     5341 gctctcacgc ctcatcgaga cggttcgccg gatcgggagg agcgaccggg tgagaggccc
     5401 gggagacttc tcagagagac ggttcgccgg atcactgagg aggagtcagg tgaaagccct
     5461 gagagacttc tcatcgagaa ggttcgctgg atcgagagag aagcgttcgg gtgagaggcc
     5521 cgaaagaggc gccggttccg caaggtccgg cgcctctttt atgtgggagt cgtcaggcag
     5581 acgctgccgg gtccgaccgc agccgcacga actgcacctg atcgtcgcgg ggcgggaggc
     5641 tgccgtccgg ggacggcacg gcccgcccct cgcggctctt gcccgcctcg ggtgccagaa
     5701 agccgcggag caggcactca cgcgcgaacc ccagcctggc cgcaagtgct tgcgaggcct
     5761 cgttgcccgg gtacgtgagc agctcgaggc gctcgacgcc gagctcgccg aaggcccagg
     5821 cgacgacgag gcgcgccgcc cccagggcta cgccccggcg gcgcgcctcc cgcttgaccc
     5881 agtagccgat ctcggcggcc tggcgctcgg cgaagcagtc caggctgacg ctgccgagca
     5941 gctcgccggc ggcgtcggtc acggccagcc gggcgcgctc gccgaggagc aggcggtggc
     6001 gcgcatcggc gatgaacgcc tcggcgtcct cgagcctgta gggagcgggc agcccgaaga
     6061 tccagtgggc gacgtctgcg tcgtcacaag cggcctgcac ggccggggcg tcgtccggat
     6121 cgaagggccg tacgagcagc cgtccgtcgg tcagtcgcgg ccagccgagg ccggcgggcc
     6181 cggcctccgt cgcccccgtc tgccacggcc gcgggtcgcc gggcacgagc gagaacagca
     6241 cgtcgtccac gcgctcgtcc ccgaccgtca tcgcccggcg cagaacgccc tcatacctgt
     6301 agccggccgc catggccgtg cgccgcgagg cgacgttgcg gacgtcggcg cggatctcga
     6361 tgcgctccag ccccaggtcg tcgaagcccc agtcggtgag cagggccacg caggccctgc
     6421 cgatcccccg ccggcgccag tccgggtgga cgatgtagcc gatctccccg cgggcgtgct
     6481 ccgtctcgag gtgcatcccg cagttggcga tgtggcggcc ggtcgccgtg tccagcgccg
     6541 cgaagttcgc ccagcgctcc tcacgccact ttcgcgcggc gtcggaggcc cactcccggc
     6601 cgtggtcgag cgtgtacggg ctcgggagcc ctgggatcca gcgtgcggcg gcctcgttgt
     6661 cgcagacggc ggcgaccgtc gcggcgtcct cgtcgcggaa cggccgcagc gtgatggcgc
     6721 ctccggagag ccgcggcagc gccgggaggt cagccgggcc gcgcacggtc cgtgcattca
     6781 gggaatgtgt cgaacgcctc gcgcgcatgc aggtcggcca agtgcgacac catgccgttc
     6841 atcgtaccgc acaagcggac ctggaccccc gccctccttg gcgtcacgcc tccgcttccg
     6901 ccgccaggga tcccagcggc tccagagatg ccggcggctc cccggcctcg ggcgcgccgt
     6961 gacgtgccgg gagccgccag gcgacgatcc ctgcaccgat caccgcgacc actgcggcgg
     7021 ccagcaagcc gtaccccagc gcggtggtga acgcatccgc cgcggccgag gcgagctgag
     7081 cgcccgcggg cgcgggcagg cgcgcggcga ccgcgtcggc gccgccgatg gacgcctcga
     7141 cgggtccgcg cagcgccgcc ggcaggccgg ccgccgcgtc gccgatgctg ctgctgtaga
     7201 tggtgctgat gagagagccg acgatcgcca cccccagcgc tccgccgacc tgccgggtca
     7261 cgtcgttcat cgcggaagcg acgccggcct tggccggcgg caccgagccc atcacggcgt
     7321 cggtcgcggg ggccatcacg gagcccatcg ccagtccgag cacgaacaac caggaggtga
     7381 ccagccagac ggagagcgcg ggtgtccaga gcacggtggc cgacaacgtg agcgcgagca
     7441 ggagcatgcc gacggcgacc acccgcttgg tgccgaactt cgccaccaga cggctgctgt
     7501 tgatggcgct gagcatcagc ccgagcgcga gcgggaccat ggtcgcgccc gcctggagcg
     7561 cactgtaccc gagcgcgaac tggaggaact gggtgagcgc gaagatcgcc gccatcagcg
     7621 agaagaagat cgtcgagatc gccagcgagc cgatgctgaa gcgcgggttc cggaagtacg
     7681 tcaggttcag catgggcgac gcgacgcggc gctcccaggc gatgaacgcg gccgtcagca
     7741 gcgcggccgc caccagcccg gcgaccacgg ccgtgctcgt ccagccgcga gacggagctt
     7801 cgatcaccga ccacacgagg gccatcaacg ccatggccga gagcgtcgcg ccgatcaggt
     7861 cgaacgcccc cggggccgga tcgcggctgt cgggcaccag cttgatgccg agcagcaggg
     7921 ccacgatcgc gaccgggacg ttgatcagga agacggagct ccacgagaag acctcgagga
     7981 gcaggccgcc ggccaggggt cccaggccga tcccgatggc cgccgtgccg gcccacacgc
     8041 cgatggcgcg gccgcgctcc tcccgcggga agacgttggt gatgatcgac agcgtggccg
     8101 gcatgatgag cgccgcgccc agccccatga ggatgcgcag cacgatgagc tggtccacgc
     8161 tgccggcgaa gaccacggcc gcgctggagc cgccgaagag gatgagaccc ccctgcagcg
     8221 cccgcttgcg cccgtagcga tcgccgagcg tccccatggt gagcagcgcc gccgcgaaga
     8281 cgagaaggta ggcatcgacg atccactgca ggtccgaggc cgaagcggtg aactggcgct
     8341 gcagcgtggg cagcgcgacg ttcacgatcg tgttgtcgag cccgatgatc atcatgctca
     8401 gagagagcac cgccagcatc tgccagcggc gctcgtagat gcgcgtcgcg ctcgtcacgg
     8461 gagctcgctt tcgtcggtgt ctggtgagaa gaactggtcg acgatccgct ccacaaggtc
     8521 gttgtcgggg tggcgtccgg cgagcaaacg ctgcatctgc agcagcgccg gaggaaccga
     8581 gctgaggagg gcgacgtcgg tctcggggga gatctcgccg cgtgcgatgg cgcgctggag
     8641 gatcgtgtcc atgccggcca tgacgggttc ggtcatgccg gcgcggaagg cgtcgccgaa
     8701 ccgctggttg gacaacgcct cgccgctgat ggccgagatc gcgtcgaagt gctgcgtgcg
     8761 gtagcgcacc agggtctgca agtaggtgag ggcgtcgccg cgcaggctgc cggtgtcggg
     8821 caccgccggc cgttccggat tgcggctgac ggcctcgagc acgacggaca ccttgtccgg
     8881 ccagcgccgg tacaggctgg cttttccgac cccggcctgc gccgcgatcc gctccaccgt
     8941 gagctgcgcg tagccttcct cggccaggag ccggagagta gccgcgacga cggcctcatc
     9001 cagtcgcgga tcccgcggcc gcccggctct tgccttcgcc gccccggcgt cttcgttgac
     9061 cttcatgcga ctcctcagtc tgtcgagcat gcacacatgc ggcgaataac gaaacgatag
     9121 cgtatcgata ctatgagcgg cgcatgctgt tgtcaaaccc ggggagccaa gaccctcgga
     9181 gtgggcccac aaacgaagag gggcggggcg gccctgccgg cctccccgcc ccgcagctcg
     9241 ttcgattcag tggaggaagt gccgccgccc cgtgaacacc atggcggcgt cgcgcgcgtc
     9301 gcaggccgcg accgcgg
//
LOCUS       DASUMX010000108         7386 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_891540, whole genome shotgun sequence.
ACCESSION   DASUMX010000108 DASUMX010000000
VERSION     DASUMX010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 7386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7386
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_891540"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..879)
                     /locus_tag="VFZ86_05360"
     CDS             complement(61..879)
                     /locus_tag="VFZ86_05360"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017540580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX5641754.1"
                     /translation="MIRHDPFADAPPDLRELLALASDLALQAGRIHAAGRRETLTLET
                     KSSPTDVVSQVDKEAERLVVDRLRAERPGDALLAEEGSLLEGESGVRWVVDPLDGTTN
                     YVYGYPAYAASIAVEVDGRAAVGVVLDSSSGRLYRAVAGHGAVCDDRPIRARELDDLS
                     RALVATGFSYDAGQRGRQGAVIAAALGRIRDIRRSGSAALDLCRVAAGEVDAFWELDL
                     SPWDRAAGALIAREAGAIVELLPAAHGSGPAVVAAHPALMPSLLALLRETGALA"
     gene            997..1344
                     /locus_tag="VFZ86_05365"
     CDS             997..1344
                     /locus_tag="VFZ86_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641755.1"
                     /translation="MAVWMPQCGLVHDTTDVATDIEVVAPAEVGEFVAAHGGRLFVWV
                     SAHPGLWFPTCLLEASLEPPHGRDLSFRRVAAPGFELYLEGTQRIWPKTMEFALRRRR
                     RVEAYWNGMAWVA"
     gene            1367..1708
                     /locus_tag="VFZ86_05370"
     CDS             1367..1708
                     /locus_tag="VFZ86_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641756.1"
                     /translation="MAPWMRKGVGVTAVTDVATDVEIVAPEDVRDFVAARGGRLYVWI
                     SVHRGIRCTLCLLETSLERPPGDLYFRRVLAPGFDLYLEATQRIWPKTMEFALRRRHV
                     AAYWNGLAWIA"
     regulatory      1783..1880
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            2065..2457
                     /gene="gcvH"
                     /locus_tag="VFZ86_05375"
     CDS             2065..2457
                     /gene="gcvH"
                     /locus_tag="VFZ86_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEX5641757.1"
                     /translation="MADETYPQELRYYKEHDWVRVEEEDAIFGITWFAQDALGEIVYA
                     ELPAVGDEVTSGKPYGELESVKAVSDVYSPLTGEVVEVNDALQDEPQRVNEDCYGEGW
                     LIRVRMTAPGELGELMDADAYKKFLDEA"
     gene            2464..3855
                     /gene="gcvPA"
                     /locus_tag="VFZ86_05380"
     CDS             2464..3855
                     /gene="gcvPA"
                     /locus_tag="VFZ86_05380"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014464491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HEX5641758.1"
                     /translation="MSSTSYVAHSADDLREMLAAVRASSVGDLFSEIPEDVRRREPLA
                     LPAGLSEQEVVAELRRLAGEDVPATQLVSFLGAGIYDHFVPAVVDAVVSRGEFLTAYT
                     PYQPERSQGVLQTIFEFQTAICELTGMDVSNASMYDGGTALAEASILAGVQTRRGEVV
                     LSAGVHPEYRQVLATESAGYGPRPRTVGLRAGAATDLDALAAAVGEGTAAVVVQQPNF
                     FGALEDVPAVAELAHAAGALLVVVADPVSLGLLEAPGRLGADIVVGEAQALGNAMNLG
                     GPGLGYMAVTTRLMRRIPGRLVGETLDLEGRRGFVLTLQTREQHIRRDKATSNICSNH
                     ALNALATLVYLSWLGKQGLPELGLHCARKAAYLRERLLALPGVAAFTEGPVFREFAVR
                     LPRPAAEVVDALVPEGFLAGVAAGRFAGQFDGDSLDDVLLVAVTEKRTRAELDAFAAA
                     LERVLAGEEADRG"
     gene            3872..5389
                     /gene="gcvPB"
                     /locus_tag="VFZ86_05385"
     CDS             3872..5389
                     /gene="gcvPB"
                     /locus_tag="VFZ86_05385"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019122793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HEX5641759.1"
                     /translation="MTRGDAPACRPAESDRTTIYEKSSPGRRAYTLPELGVPEVPAAE
                     LVPEALRRSAPARLPEIGEIDLLRHFTGLSTLNYGVDSGSYPLGSCTMKYNPRVNEVA
                     ARLDGFSGIHPYQPESLLQGALELMHELERWLAEISGLHATTLQPAAGAHGELTGLLV
                     IRAAHLAEGRDPKRIIIPDTAHGTNPASVVMAGFVPAPVSSDARGGVDIDVLRAIIDE
                     GDIAGLMLTNPNTLGLFDEHITEIARLVHDAGGLLYYDGANANAVLGISRPGDMGFDI
                     VHFNTHKTFSTPHGGGGPGAGPIVVRDSLAPFLPVPLVEKDADGRFFFQYDRPQSIGK
                     VRTFYGNFGVLVRAWTYIRSLGAEGLLEVSETAVLNANYVMEGLRGLLDLPYGDRRCM
                     HEFVGSATPLKQDGVRALDVAKRLLDYGVHPPTTYFPLIVDEALMVEPTETESKDALD
                     RLVEAFRSVAREAKEQPELLREAPVTLPVRRLDEAQAARHPVVKQRFPEDAEGGA"
     gene            5451..6131
                     /locus_tag="VFZ86_05390"
     CDS             5451..6131
                     /locus_tag="VFZ86_05390"
                     /inference="COORDINATES: protein motif:HMM:NF015084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641760.1"
                     /translation="MSIVKILPDFRSGGGAQMALDAGLLETVDEVIARRYTWAPPALS
                     VGKFQELQTVPGLPFEVVRRPSGGRAVLHGEAFEWSFAVAFPPGVPGGGPRAAVDVGG
                     PYGLVAGAFAAALVELGLTLDGSGEVPYQRSALCFAGALRYDICSRGEKVVAIAQAKK
                     EGRTLVHGSFLMERPPVELTLATEALVGENWEAEGLAGAGVMLAPETIWNTTLRRLEA
                     ALRRAAST"
     gene            6142..>7386
                     /gene="lpdA"
                     /locus_tag="VFZ86_05395"
     CDS             6142..>7386
                     /gene="lpdA"
                     /locus_tag="VFZ86_05395"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEX5641761.1"
                     /translation="MTKVLVLGGGPAGYVAAGRAAQLGAEVTVVEAREIGGTCLNRGC
                     VPTKAMVAGAARLHEARRAVEYGVQVGEVSLDFAAFMARKDAATTQLRDGVAHLLKAR
                     RVQVVAGQATLAGPGRLALAAGATLPDGAVLEEGGELEGDAVILASGSEPVRMGLFDW
                     SDPRVMTSDELLEIDAVPASLAIVGGGVIGCEFASVFSRLGTQVTVIEMLPQLLPGED
                     QRVGRTLQQAFKKAGIAAHVKTAVEAAEAGAAGVTLRLAGGAEITAERVLVAVGRRPV
                     SAGLGYEEAGVEIGPGGFVVTDESCRTTLAGVFAAGDVAGPPLLAHWAYHQGAIAAEN
                     AVTGSDLHCDRTLVPSCTFSSPEVASCGITEDRAKAEGIAHEVAHVRFNGNSKAVCDG
                     DADGFVRIVCEPGGGKVLGASLI"
BASE COUNT          982 a         2691 c         2738 g          975 t
ORIGIN      
        1 gctgcgcggc tgatcccgcg gccggccgcc cccggtcgct gcggtgccgc gggctctctg
       61 ctacgccagc gcgcccgtct cgcgcagcag cgccagcaac gacggcatga gcgcgggatg
      121 ggcggccacc acggccggcc cggagccgtg cgccgccggc agcagctcga cgatggcccc
      181 ggcctcgcgt gcgatcaagg cccctgcggc ccggtcccac ggcgagaggt cgagctccca
      241 gaaggcgtcg acctcgccgg cggcgacgcg gcagaggtcg agcgccgcgc tgccgctgcg
      301 gcggatgtcg cggatgcggc ccagcgccgc ggcgatcacc gcgccctgcc ggccgcgctg
      361 ccccgcgtcg taggagaagc cggtggccac gagcgcgcgg gagaggtcgt cgagctcccg
      421 ggcgcggatc gggcggtcgt cgcagacggc gccgtgcccg gcgaccgccc ggtacagcct
      481 gccggagctg gagtccagga cgacgcccac ggccgcgcgc ccgtccacct ccacggcgat
      541 ggaggccgcg taggcggggt agccgtacac gtagttcgtg gtgccgtcga gcgggtcgac
      601 gacccagcgg accccgctct cgccctccag caggctgccc tcctcggcga gcagcgcgtc
      661 gcccggacgc tccgcgcgca ggcggtcgac gacgagccgc tccgcctcct tgtccacctg
      721 gctgacgacg tcggtggggg acgacttggt ctcgagggtg agcgtctccc ggcggcctgc
      781 ggcgtggatg cgtcctgcct gcagcgcgag gtcgctcgcc agtgcgagca gctcgcgcag
      841 gtcgggcggg gcgtcggcga aggggtcgtg ccggatcatc ggccgacggt accagacggt
      901 ccggcgcggc tacaaggcgc cctccaggca catcgtggat tgtgatccgc gatgtgagga
      961 acacaccagt tgacggccag tcttcgcgga ggagccatgg cggtctggat gccacagtgc
     1021 ggtctggtgc acgacacgac cgatgtcgcc acggacatcg aggtcgtggc gcccgccgag
     1081 gtgggcgagt tcgtcgccgc gcacggcggg cgtctcttcg tctgggtctc ggcgcatccc
     1141 ggcctctggt tccccacctg cctgctggag gcgtccctcg agcctccgca cgggcgagac
     1201 ctctccttcc gccgcgtcgc ggctcccgga ttcgagctct acctcgaggg gacgcaacgc
     1261 atctggccga agacgatgga gttcgcgctt cgccgccggc gccgggtgga ggcgtactgg
     1321 aacggcatgg cctgggtcgc ctgaccccgg caggcgcgga gcggccatgg ccccctggat
     1381 gagaaagggc gtcggagtga ccgccgtcac ggacgtcgcc accgacgtcg agatcgtggc
     1441 gcccgaggac gtgcgcgact tcgtcgccgc ccgcggcggg cgcctctatg tctggatctc
     1501 ggtgcaccgc ggcatacgct gcacgctgtg cctcctcgag acctcgctgg agcgtccccc
     1561 cggggacctg tacttccgcc gggtcctggc tcccggcttc gacctgtacc tcgaggccac
     1621 gcagcggatc tggccgaaga cgatggagtt cgccctccgg cgccggcatg tcgcagcgta
     1681 ctggaacggt cttgcctgga tcgcctgacg ggtgtctccc gttgtccggc cggcacggcc
     1741 ggtgctacag tggaggcgct gagccggtgc gaagcagccc gggcgaaggc cgcgtgagag
     1801 tccgcctcgc aggcgggcgc cgaaggggca aggcgcgtgg atgcgccgaa actctcaggc
     1861 aaacggagcg cggccggacg ggcccccgga tgacgaggcg ccgccgccgt ggcgccggag
     1921 gcagacaggg tggatactcc accctgtctt tttgttttcc ggcgcgggag cggcggcaac
     1981 ccggccgcga gcgtttctcg agcgcgcggc ccgggcaggt tcgagagtcg aatggcgagc
     2041 gagcgggacg accagggagg cacgatggcc gacgagacgt atcctcagga gctgcggtac
     2101 tacaaggagc acgactgggt gcgcgtcgag gaagaggacg cgatcttcgg catcacgtgg
     2161 ttcgcgcagg acgcgctggg tgagatcgtc tacgcagagc tgcccgcggt gggcgacgaa
     2221 gtgacgtccg gcaagcccta cggtgagctc gagtcggtca aggcggtgag cgacgtgtac
     2281 tccccgctca ccggcgaggt cgtggaggtc aacgacgcgc tccaggacga gccgcagcgg
     2341 gtcaacgagg actgctacgg cgagggctgg ctcatccgcg tgcgcatgac cgcccccggc
     2401 gagctcggcg agctcatgga cgccgacgcc tacaagaagt tcctcgacga ggcctgacac
     2461 gccgtgagct ccaccagcta cgtcgcccac agcgcagacg acctgcggga gatgctggcg
     2521 gccgtcaggg cctccagcgt cggcgacctc ttctcggaga tcccggagga cgtgcgccgg
     2581 cgggagccgc tggcgctgcc cgccggcctc agcgagcagg aggtcgtcgc cgagctgcgc
     2641 cgcctcgccg gcgaggacgt gccggcgacc cagctggtga gcttcctcgg cgccggcatc
     2701 tacgaccact tcgtccccgc cgtggtcgac gccgtggtca gccgcgggga gttcctcacc
     2761 gcctacacgc cctaccagcc ggagcgcagc cagggcgtgc tgcagacgat cttcgagttc
     2821 cagacggcca tctgcgagct gaccggcatg gacgtcagca acgcctccat gtacgacggc
     2881 ggcacggcgc tcgccgaggc ctcgatcctc gccggcgtgc agacccggcg cggcgaggtc
     2941 gtgctctccg ccggcgtgca ccccgagtac cggcaggtgc tcgccacgga gtcggccggg
     3001 tacgggccgc gcccgcggac cgtgggtctg cgggcgggcg cggccaccga cctggacgcg
     3061 ctcgccgccg cggtcggcga aggcacggcg gcggtcgtcg tccagcagcc gaacttcttc
     3121 ggcgccctcg aggacgtgcc cgccgtcgcc gaactggcgc acgcggcggg cgcgctgctc
     3181 gtcgtggtcg ccgacccggt ctcgctcggg ctcctcgagg cgcccggcag gctcggcgcc
     3241 gacatcgtcg tcggggaggc gcaggcgctg ggcaacgcca tgaacctcgg cggccccggc
     3301 ctcggctaca tggccgtcac caccaggctc atgcgtcgca tccccggccg cctggtcggc
     3361 gagaccctcg acctcgaggg gcggcgcggg ttcgtgctca cgctgcagac gcgcgagcag
     3421 cacatccgcc gcgacaaggc gacgagcaac atctgctcca accacgcgct caacgcgctg
     3481 gccacgctcg tctacctgag ctggctgggc aagcagggcc tgccggagct cggcctgcac
     3541 tgcgcgcgca aggccgccta cctgcgcgag cgcctgctgg cgctcccggg cgtggcggcc
     3601 ttcacggagg gtcccgtgtt ccgcgagttc gccgtgcggc tgccgcggcc ggcggccgag
     3661 gtggtcgacg cgcttgtgcc ggagggcttc ctggccggcg tggccgcggg ccgcttcgcc
     3721 ggccagttcg acggggactc actcgacgac gtcctcctcg tggccgtgac cgagaaacgc
     3781 acgcgcgccg agctggacgc cttcgccgcc gcgctcgagc gcgtcctggc cggcgaggag
     3841 gcggaccgtg gctgagcgcg gcgacacgcc cgtgacccgc ggcgacgcgc ccgcctgccg
     3901 cccggccgag agcgaccgca cgaccatcta cgagaagtcc agcccgggcc ggcgcgcgta
     3961 cacgctgccg gagctcggcg tccccgaggt gccggccgcc gagctcgtcc cggaggccct
     4021 ccgccgctcc gcgccggcgc gcctgcccga gatcggcgag atcgacctgc tgcgccactt
     4081 caccgggctc tcgacgctca actacggcgt ggacagcggc agctacccgc tgggctcgtg
     4141 cacgatgaag tacaacccgc gcgtcaacga ggtggcggcg cggctcgacg gcttctccgg
     4201 catccacccc taccagccgg agtcgctgct gcagggtgcg ctcgagctga tgcacgaact
     4261 cgagcgctgg ctcgccgaga tctccggtct gcacgcgacc acgctgcagc cggcggccgg
     4321 agcgcacggc gagctcaccg gcctgctggt gatccgcgcg gcgcacctgg ccgaaggccg
     4381 cgaccccaag cgcatcatca tcccggacac ggctcacggc acgaaccccg cgagcgtcgt
     4441 catggccggt ttcgtgcccg cccccgtctc gagcgacgcg cgcggcggcg tggacatcga
     4501 cgtgttgcgc gcgatcatcg acgagggcga catcgccggc ctcatgctca ccaaccccaa
     4561 cacgctcggc ctcttcgacg agcacatcac cgagatcgcc cggctggtgc acgacgccgg
     4621 cggcctgctg tactacgacg gcgccaacgc caacgccgtg ctcggcatca gccgccccgg
     4681 cgacatgggc ttcgacatcg tccacttcaa cacgcacaag acgttcagca cgccgcacgg
     4741 cggcggcggc cccggcgccg gccccatcgt ggtccgcgac tccctggcgc cgttcctgcc
     4801 ggtgccgctg gtggagaagg acgcggacgg gcgcttcttc ttccagtacg accgtccgca
     4861 gtccatcggc aaggtgcgca ccttctacgg caacttcggg gtgctggtcc gcgcctggac
     4921 gtacatccgc agcctgggcg cggagggcct gctcgaggtc agcgagacgg cggtgctcaa
     4981 cgccaactac gtgatggagg ggctgcgcgg gctgctcgac ctgccgtacg gtgaccggcg
     5041 ctgcatgcac gagttcgtgg gttcggcgac gccgctcaag caggacggcg tgcgcgccct
     5101 cgacgtggcg aagcgcctgc tcgactacgg cgtgcacccg ccgaccacgt acttcccgct
     5161 catcgtcgac gaggcgctca tggtcgagcc cacggagacg gagtccaagg acgcgctcga
     5221 ccgcctggtc gaggcgttcc ggagcgtggc gcgcgaggcg aaggagcagc cggagctgct
     5281 gcgcgaggcg ccggtgacgc tgccggtgcg gcggctcgac gaggcgcaag ccgcccgcca
     5341 ccccgtggtg aagcagcggt tcccggagga cgcggagggc ggcgcctgag gctgcgcccc
     5401 cgcgccgccc gccgccacct cccgagtcgc cgaggccccg aggcgccgcc gtgagcatcg
     5461 tcaagatcct gccggacttc cgctcgggcg gcggcgccca gatggcgctc gacgccgggc
     5521 tgctcgagac ggtggacgag gtgatcgcgc gccggtacac gtgggcgccg ccggcgctct
     5581 ccgtcggcaa gttccaggag ctccagacgg tgcccggcct gccgttcgag gtggtgcgcc
     5641 gccccagcgg cgggcgggcg gtgctgcacg gcgaggcgtt cgagtggtcg ttcgccgtgg
     5701 cgttcccgcc gggcgtcccg ggcggcggcc cgcgggcggc ggtggacgtc ggcggcccgt
     5761 acggcctcgt cgccggcgcg ttcgccgccg cgctcgtgga gctcgggctg accctggacg
     5821 gcagcggcga ggtgccgtac cagcggagcg ccctctgctt cgccggcgcg ctccgctacg
     5881 acatctgctc gcgcggcgag aaggtcgtcg ccatcgccca ggccaagaag gaggggcgca
     5941 cgctcgtgca cggctccttc ctcatggagc ggccgccggt cgagctcacg ctcgccaccg
     6001 aggcgctggt gggcgagaat tgggaggccg agggtctggc gggcgcgggg gtcatgctcg
     6061 ccccggagac gatctggaac acgacgctgc ggcgtctcga ggccgctctg cggagagcgg
     6121 cctccacctg aggaggaacg catgaccaag gtcctggtgc tcggcggcgg ccccgccggc
     6181 tacgtcgccg ccggccgcgc cgcccagctc ggcgccgagg tgaccgtcgt ggaggcgcgc
     6241 gagatcgggg gcacctgcct gaatcgcggc tgcgtcccga ccaaggcgat ggtggcgggc
     6301 gcggcgcgcc tgcacgaggc gcggcgcgcg gtcgagtacg gcgtgcaggt cggtgaggtc
     6361 tccctcgact tcgccgcctt catggcgcgc aaggacgcgg cgacgacgca gctgcgcgac
     6421 ggcgtcgccc acctgctcaa ggcgcgcagg gtgcaggtgg tggccgggca ggctacgctc
     6481 gccggccccg gccgcctggc cctcgcggcc ggcgcgaccc tgccggacgg cgccgtgctc
     6541 gaggagggtg gcgagctcga gggcgacgcg gtgatcctcg cgagcggctc tgagccggtg
     6601 cgcatgggcc tcttcgactg gtccgacccc cgggtcatga ccagcgacga gctgctcgag
     6661 atcgacgccg tccccgcctc cctcgccatc gtcggcggcg gcgtgatcgg ctgcgagttc
     6721 gccagcgtct tcagccgcct cgggacccag gtcaccgtga tcgagatgct cccccagttg
     6781 ctgcccgggg aggaccagcg cgtgggcagg accctgcagc aggcgttcaa gaaggccggc
     6841 atcgcggcgc acgtcaagac ggccgtcgag gcggcggagg ccggcgccgc cggcgtcacg
     6901 ctgcgcctcg caggcggcgc cgagatcacc gccgagcgcg tgctcgtggc ggtcggccgc
     6961 agacccgtga gcgccggcct cggttacgag gaggccggcg tcgagatcgg ccccgggggc
     7021 ttcgtcgtca cggacgagag ttgccggacc acgctcgccg gcgtgttcgc ggcgggcgac
     7081 gtcgcgggcc cgccgctgct cgcgcactgg gcgtaccacc agggcgcgat cgccgcggag
     7141 aacgcggtca ccggatccga cctgcactgc gaccggacgc tcgtccccag ctgcacgttc
     7201 agctcgccgg aggtggcgag ctgcggcatc accgaggaca gggcgaaggc cgagggcatc
     7261 gcgcacgagg tcgcccacgt gcgcttcaac ggcaactcca aggcggtctg cgacggcgac
     7321 gccgacgggt tcgtgcgcat cgtctgcgag cccggcggcg gcaaggtcct cggcgccagc
     7381 ctgatc
//
LOCUS       DASUMX010000109         9033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1754739, whole genome shotgun sequence.
ACCESSION   DASUMX010000109 DASUMX010000000
VERSION     DASUMX010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 9033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9033
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1754739"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="VFZ86_05400"
     CDS             complement(<1..596)
                     /locus_tag="VFZ86_05400"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEX5641762.1"
                     /translation="MNDKQDSILYLSRDDLGALGLTMLEVVDAVEAGCAAKGRGQVVM
                     PPKLSLHGEGDAYAQVMAATLPGDGGSGAKWVTLFPQNAGRGLPITNGLVVLSDPVTG
                     LPAAVMDAATVTAWRTGASVGVAARHLARPDAATVGVLGCGVQARASVRALAAVLRGL
                     REVRCHDAVGAAGEAFVAGLGAELPDLRLTTVATPEDAV"
     gene            674..1879
                     /locus_tag="VFZ86_05405"
     CDS             674..1879
                     /locus_tag="VFZ86_05405"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX5641763.1"
                     /translation="MEDTTKILLEIFLAFAGARLLGWMFQSVKQPAIIGELLAGAILG
                     PALLGLVHQSEFLEALGELGVILLLFTAGLDTHLEQLASAQATAAKVALFGAALPFAA
                     GVAGGLAFQYAVGESLFLGTALMATSVGITVRVLRDLGYHRRRSVTIILGAAVLDDVL
                     GLIVLGLVTALALGEANTLQLVLLAVEAVVFVGGVGFVGPQIVNRFGARLAQLSANTI
                     FELGIILMLALSLLANYIGLAAIIGAFIAGLVVAELKQHTEVESKFTPLAWFFVPFFF
                     VLMGTYIDFASFAKPVVLLAIAAFTAIAVVTKYLGAIWGARSEGKRIAREVGVGMVPR
                     GEVGIVVAGIALAAGVVSEDAYAAVLGMVLATTVLSPYLIRAVFSRRKPSSEKPEPDP
                     GADAGATPG"
     gene            complement(1876..2412)
                     /locus_tag="VFZ86_05410"
     CDS             complement(1876..2412)
                     /locus_tag="VFZ86_05410"
                     /inference="COORDINATES: protein motif:HMM:NF013798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HEX5641764.1"
                     /translation="MKTSVGKTKLELASGDITVLEVDAIVTPSGTELWMDHGVAAAVK
                     RAGGEAVEKEAVLQGPVQIGEAVVTTGHDLAARWVIHVALTDAGPQADAAGIAKATRA
                     ALAGAERCHARSLAVPAFGTGGGGFPLYQSASIMVTETVAYLKERKNTALRHIMFSVY
                     DEAARAAFKNAMVGISRF"
     gene            2526..3530
                     /locus_tag="VFZ86_05415"
     CDS             2526..3530
                     /locus_tag="VFZ86_05415"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX5641765.1"
                     /translation="MAAAFGMTALWALVVVVNKKILESVTPLAVNFLIRLVAIAGLLL
                     LTVPLTVFGLWSNGFGIDAESAGWIALSAVATWLIAFNAYYYALRGARVAVVAPICST
                     DPIWTAFFAWLLLGSAFGAPTFVGMAVAMTGVALISRWMGEGGAADGELAGAGALAAA
                     AHGDVLPDGRCAAPAPAAPAGPPPATRATLIGLAVLTAAGWGLGPVFIDQAATAYGRA
                     TASMMLLSQALGALMLGGVLLARRSPLACRPLTPATRRRVTWLIVLAGMLEAAVSVLF
                     YLSIVTLGPVLAMLIMATTPVFSIVFGVVLLRERPGLRLTLAAVVTVAGVLIATLDGL
                     "
     gene            complement(3540..4265)
                     /locus_tag="VFZ86_05420"
     CDS             complement(3540..4265)
                     /locus_tag="VFZ86_05420"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF004616.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEX5641766.1"
                     /translation="MRTVTFHVKRYDPARDEKGAARIQSYEIPYETSLTVLEGLFHIQ
                     ENLDGSLAFRYCCRASICGSCAMYINGRYRLACQTNIVHLHTDQVDVQPLPHLPLVKD
                     LVCDMTDFFAKYEYIQPWLIRRSEPPAKEMLQSPADRHKLNMPIDCILCGACYSSCPS
                     VWTEENYLGPAALLKAYRFEVDSRDEARKDRLPQWDNERGVYRCHTITNCSEACPKEL
                     NPTEGIQWLKMAAVRRRLFGKAK"
     gene            complement(4265..5971)
                     /locus_tag="VFZ86_05425"
     CDS             complement(4265..5971)
                     /locus_tag="VFZ86_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012458982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5641767.1"
                     /translation="MPHHQLIVVGGGLAGLRAAVEARAAGLDVAVLSQVHPGRSHSSA
                     AQGGINAALANHPDAHDDTPEKHAFDTVKGSDYLADQDAAIQMTSDAPGVIFEMDHWG
                     CPFSRLDDGRIAQRPFGGAGYPRTCYGADKTGHYLLHTLVERAYERRIQMYVEVFVTA
                     LIVDDGRCRGVVAYDMIRGGFHAFTADAVVLATGGAGRIYANSTNAIISTGGGAAMAY
                     HAGVPMEDMEFIQFHPTGLLTTHILMSEACRGEGGYLVNDEGERFMARYAPKAMELAP
                     RDITARSITTEINEGRGIGGADFVHLDLRHLGAERILERLPGIRDIAIHFEGVDPIEE
                     PIPIKPVQHYTMGGIDTDVDGRTRLPGLYAAGECACVSVHGANRLGGNSLLETVVFGR
                     RAGMAVVADLEGGSPAGDASAAGRAAVAAVEARVGELAARGADGADAYAIRAEMIATT
                     RDYFGVFRDDAVMRTGLETLRRLKERCSRIGLRNAGGVFNLDLMRTLELEGMVDLALC
                     VAEGAWARTESRGSHSRTDHPARDDERWLKHTLAHYTPEGPRLEYVPVTLGTFEPQER
                     KY"
     gene            complement(5982..6344)
                     /locus_tag="VFZ86_05430"
     CDS             complement(5982..6344)
                     /locus_tag="VFZ86_05430"
                     /inference="COORDINATES: protein motif:HMM:NF013307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641768.1"
                     /translation="MRTETLDRSRGGMWPWLGQRVTAVVIIVTIMVHLVLTHFVAIGE
                     LSFDDIGERLASTAVLVNDLLLLVAVVYHALNGIRMVALDWGLTGSGLRRGFDAVLWI
                     AGIVAVAYGTWALWVWVG"
     gene            complement(6355..6741)
                     /gene="sdhC"
                     /locus_tag="VFZ86_05435"
     CDS             complement(6355..6741)
                     /gene="sdhC"
                     /locus_tag="VFZ86_05435"
                     /inference="COORDINATES: protein motif:HMM:NF013307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEX5641769.1"
                     /translation="MSATERETANVGSGLWKGIGMWAWILFRISGLVLAVYLFVHIWV
                     ISQGRVSGAETLDDFFAFFDKPFLVFLDSVLVAAVLYHALNGVRILLMDVGIGIHRHK
                     AVFWVCMLVAALAVWGFAYRAIPLIF"
     gene            complement(6846..7658)
                     /locus_tag="VFZ86_05440"
     CDS             complement(6846..7658)
                     /locus_tag="VFZ86_05440"
                     /inference="COORDINATES: protein motif:HMM:NF040120.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6498-containing protein"
                     /protein_id="HEX5641770.1"
                     /translation="MSERDTSQGTDPRPPATAGDARGAAVASPVPTGATLARAVPSFA
                     VLLVADVAVVWAAVTGSWTALVVVVACVIDGLADGIFAWLRARASFAAGAATDDRDAV
                     LVREFVRTYLIVVGAQAVIAYMVFSGMLLKPGGEAPDAPGAAFVTWQYWAVVGGLVAM
                     RAFLYWWDFARGGEAAVIPPEAAVAEPLRRLFVLQFGVLAGALLVYWLFDSSLTGLAV
                     LLVAKTAADLVLAVLERLRAARIVAAVDAGARPARRPAPRQRARRGGRKRRR"
     gene            complement(7819..8478)
                     /locus_tag="VFZ86_05445"
     CDS             complement(7819..8478)
                     /locus_tag="VFZ86_05445"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class F sortase"
                     /protein_id="HEX5641771.1"
                     /translation="MKNKRARLSASRRAAVLVLVVVAAAALAAGVYLIVAPDGSTESP
                     AANAAELTDPAQEPATPAAVPKVTYGLPVRLIIPRLGVNAAVEKVGLTSRRDMASPSR
                     ADTVGWYKFGPRPGNEGSAVIDGHSGYADDREAVFDDLPKLKVGDKVFVKDAKGKRLA
                     FVVRRKVLFGRNADAGEVFGSTKHRRLNLITCTGSFDVAAGTHAKRLVVFTELQPRDG
                     R"
     gene            complement(8525..8692)
                     /locus_tag="VFZ86_05450"
     CDS             complement(8525..8692)
                     /locus_tag="VFZ86_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641772.1"
                     /translation="MTDEDEDAEDVGFPATGYPPSDGGSTSWLLVSGAFAAGLTLLFA
                     AWRLHPARRHN"
BASE COUNT         1219 a         3197 c         3274 g         1343 t
ORIGIN      
        1 acggcgtcct ccggcgtggc gaccgtcgtg agccgcaggt ccggcagttc cgcgccgagg
       61 ccggcgacga aggcctcgcc ggccgcgccg acggcatcgt ggcaacgcac ctcgcgcagc
      121 ccgcgcagca cggccgcgag cgcccgcacg gacgcgcggg cctggacgcc gcagccgagc
      181 acgcccacgg tcgcggcatc agggcgcgcc aggtggcgcg cggcgacacc cacactcgcg
      241 ccggtgcgcc acgccgtcac ggtggctgca tccatcaccg ccgccggcag ccccgtcacc
      301 gggtcgctga gcacgaccag gccgttggtg atcggcaagc cgcggccggc gttctgaggg
      361 aacagggtga cccacttggc gcccgacccg ccgtcccccg gcagcgtcgc cgccatgacc
      421 tgcgcgtagg cgtcgccctc gccgtgaagg ctgagtttgg gcggcatgac gacctgtccc
      481 ctgcccttgg cggcgcagcc cgcctcgacg gcgtcgacga cctcgagcat cgtcaggccg
      541 agcgcaccga ggtcgtcacg ggagaggtag aggatcgagt cctgcttgtc gttcacggcg
      601 cccggccacc tccggtgagc aacaggtcac ggacactggg tcatacgccc ggactatata
      661 ctcagggccg gtcatggagg acacgaccaa gatcctgctg gagatcttcc tggcgttcgc
      721 cggcgcgagg ctgctcggct ggatgttcca gtccgtcaag cagccggcga tcatcgggga
      781 gctcctcgcg ggcgcgatcc tcggcccggc gctgctcggc ctcgtgcacc agtccgagtt
      841 cctcgaggcg ctcggcgagc tcggcgtgat cctcctgctc ttcacggccg gcctggacac
      901 gcacctcgag cagctcgcga gcgcgcaggc gacggcggcc aaggtggccc tgttcggcgc
      961 cgcgctgccg ttcgccgccg gcgtcgccgg cggcctcgcc ttccagtacg ccgtcggcga
     1021 gagcctgttc ctggggaccg cgctcatggc gacctcggtc gggatcaccg tgcgcgtgct
     1081 ccgcgacctg gggtaccacc gccgccggtc cgtcaccatc atcctcgggg cggcggtcct
     1141 cgacgacgtg ctgggactga tcgtcctggg cctcgtcacc gccctcgccc tcggcgaggc
     1201 gaacaccctc cagctcgtcc tcctggcggt ggaagcggtg gtgttcgtcg gcggcgtcgg
     1261 cttcgtcggc ccgcagatcg tgaaccgctt cggcgcccgc ctggcgcagc tctccgccaa
     1321 cacgatcttc gagctgggca tcatcctgat gctcgccctc tcgctcctcg ccaactacat
     1381 cggcctcgcg gccatcatcg gcgcgttcat cgccggcctc gtcgtcgccg agctcaagca
     1441 gcacacggag gtggagagca agttcacccc gctcgcgtgg ttcttcgtgc cgttcttctt
     1501 cgtgctgatg ggcacctaca tcgacttcgc ctcgttcgcg aagccggtcg tgctcctggc
     1561 catcgccgcg ttcaccgcga tcgccgtggt gacgaagtat ctgggagcga tctggggagc
     1621 caggagcgag ggcaagcgca tcgcccgcga ggtcggggtc ggcatggtgc cgcgcggcga
     1681 ggtcggcatc gtcgtcgccg ggatcgctct ggccgccggc gtcgtgagcg aagacgccta
     1741 cgcggccgtg ctggggatgg tcctggcgac gaccgtcctc agcccgtacc tgatccgcgc
     1801 cgtcttctcg cgccgtaagc cgtcgtccga gaagcctgag ccggatccag gcgcagacgc
     1861 cggagcgacg cccggctaga agcggctgat gcccaccatg gcgttcttga acgccgcccg
     1921 ggccgcctcg tcgtagacgg agaacatgat gtggcgcagc gcggtgttct tgcgctcctt
     1981 gaggtacgcc accgtctccg tgaccatgat gctggcggac tggtagagcg ggaacccgcc
     2041 cccgccggtg ccgaacgccg gcacggcgag agagcgcgcg tggcagcgct ccgccccggc
     2101 cagcgcggcg cgcgtggcct tggcgatacc ggccgcgtcg gcctgcgggc cggcgtcggt
     2161 gagcgccacg tggatgaccc agcgggccgc gaggtcgtgg ccggtggtga cgacggcctc
     2221 gccgatctgc accggcccct ggagcacggc ctccttctcg accgcctcgc cgccggcgcg
     2281 cttgacggcc gcggccacgc cgtgatccat ccagagctcc gtccccgacg gcgtgacgat
     2341 cgcgtccacc tcaagcaccg tgatgtcgcc gctggcgagt tcgagcttgg tcttgcccac
     2401 tgacgtcttc atctggcctc cgctctgcgc ccccggcgca ggtatccgtt ccggggagta
     2461 tacctgtcgt gcggtagaat cgcggggagc cttggggggt cgccacgagt ccgtacagca
     2521 cctggatcgc cgctgccttc ggcatgaccg cgttgtgggc gctggtggtc gtcgtcaaca
     2581 agaagatcct ggagtccgtc actcccctcg cggtcaactt cctcatccgc ctcgtggcca
     2641 tcgccggcct tctcctgctc accgtgccgc tcaccgtgtt cggcctgtgg agcaacggct
     2701 tcggcatcga cgcggagtcc gccggctgga tcgcgctctc ggccgtggcg acgtggctca
     2761 tcgcgttcaa cgcctactac tacgcgctgc gcggagcacg cgtcgccgtg gtcgcgccca
     2821 tctgcagcac cgaccccatc tggacggcct tcttcgcctg gctgctgctc ggcagcgcct
     2881 tcggggcgcc gacgttcgtc ggcatggccg tggcgatgac cggcgtcgcg ctgatctccc
     2941 gctggatggg cgagggcggc gcggcggacg gcgagctggc cggcgccggc gcgctcgccg
     3001 cggcggcgca cggagacgtg ctgcccgacg ggcgctgcgc ggcgccggct ccggcggcgc
     3061 cggccgggcc gccgccggcg accagggcca ccctcatcgg gctcgccgtg ctcaccgcgg
     3121 ccggctgggg cctcggcccc gtcttcatcg accaggccgc gaccgcctac ggccgcgcca
     3181 ccgcgagcat gatgctgctc agccaggcgc tcggtgcgct catgctgggc ggcgtgctgc
     3241 tggcgcgccg ctcacccctc gcgtgccggc cgctcacgcc ggcgacgcgg cgacgggtga
     3301 cctggctcat cgtcctggca ggcatgctgg aggcggcggt gagcgtgctc ttctacctca
     3361 gcatcgtcac gctcgggccg gtgctggcga tgctgatcat ggcgacgacg ccggtgttct
     3421 cgatcgtctt cggcgtcgtc ctgctgcgcg agcggcccgg cctgcggctc acgctcgcgg
     3481 ccgtggtcac cgtggccggc gtgctcatcg ccacgctcga cggcctctga ccgcgcgcgt
     3541 cacttcgcct tgccgaacaa cctccggcgc acggccgcca tcttgagcca ctggatgccc
     3601 tccgtggggt tcaactcctt cgggcaggcc tccgagcagt tggtgatggt gtggcagcgg
     3661 tagacgccgc gctcgttgtc ccactgcggc aagcggtcct tgcgcgcctc gtcacgggag
     3721 tcgacctcga accggtacgc cttgagcagc gccgccggcc ccaggtagtt ctcctcggtc
     3781 cacaccgacg ggcagctgct gtagcaggcg ccgcagagga tgcagtcgat gggcatgttg
     3841 agcttgtggc ggtctgccgg cgactgcagc atctccttcg ccggcggctc gctcctgcgg
     3901 atgagccacg gctggatgta ctcgtacttg gcgaagaagt cggtcatgtc gcagaccagg
     3961 tccttgacca gcggcaggtg cggcagcggc tggacgtcca cctggtcggt gtgcaggtgc
     4021 acgatgttgg tctggcaggc gagccggtag cggccgttga tgtacatcgc gcacgagccg
     4081 cagatggagg cgcggcagca gtagcggaac gccagcgagc cgtccaggtt ctcctggatg
     4141 tggaacaggc cctcgaggac ggtgaggctc gtctcgtagg ggatctcgta ggactggatg
     4201 cgcgcggcgc ccttctcgtc ccgggccggg tcgtagcgct tgacgtggaa ggtgacggtc
     4261 cgcatcagta cttccgctcc tgtggctcga aggtgccgag ggtgacgggc acgtactcga
     4321 gccgcggtcc ctcgggcgtg tagtgcgcca gcgtgtgctt cagccagcgc tcgtcgtcgc
     4381 gcgccgggtg gtccgtgcgc gagtgcgagc cgcgcgactc ggtgcgcgcc cacgcgccct
     4441 cggccacgca gagggcgagg tcgaccatgc cctcgagctc gagcgtgcgc atgaggtcga
     4501 ggttgaacac gccgccggcg ttgcgcagcc cgatccggct gcagcgctcc ttgaggcggc
     4561 gcagggtctc gaggccggtg cgcatcacgg cgtcgtcgcg gaacacgccg aagtagtcgc
     4621 gcgtcgtggc gatcatctcg gcgcggatgg cgtaggcgtc ggccccgtcc gcgccgcgcg
     4681 ccgccagctc gccgacgcgc gcctccacgg cggccacggc ggcgcggccg gcggcggacg
     4741 cgtcgcccgc cggcgatccg ccctcgaggt cggccacgac ggccatgccg gcgcggcgcc
     4801 cgaagacgac ggtctcgagc agcgagttgc cgccgagccg gttggcgccg tggacgctga
     4861 cgcaggcgca ctcgccggcg gcgtagagcc ccggcaaccg cgtgcggccg tcgacgtcgg
     4921 tgtcgatccc gcccatggtg tagtgctgca ccggcttgat ggggatgggc tcctcgatgg
     4981 ggtccacgcc ctcgaagtgg atggcgatgt cgcggatgcc cggcagccgc tcgaggatcc
     5041 gctcggcgcc gaggtgccgg aggtcgaggt gcacgaagtc ggcgccgccg atgccgcggc
     5101 cctcgttgat ctcggtggtg atcgagcggg cggtgatgtc ccgcggggcc agctccatcg
     5161 ccttgggcgc gtacctggcc atgaagcgct cgccctcgtc gttgacgagg tagccgccct
     5221 ctccgcgaca ggcctcgctc atgaggatgt gcgtggtcag caggccggtg gggtggaact
     5281 ggatgaactc catgtcctcc atcggcacgc cggcgtggta ggccatcgcc gcaccgccgc
     5341 cggtgctgat gatggcgttg gtggagttgg cgtagatgcg gccggcgccg cccgtggcca
     5401 ggaccacggc gtcggccgtg aacgcgtgga agccgccgcg gatcatgtcg taggcgacga
     5461 cgccgcggca gcggccgtcg tcgacgatga gcgcggtcac gaacacctcg acgtacatct
     5521 ggatccggcg ctcgtaggcg cgctcgacga gcgtgtgcag caggtagtgg ccggtcttgt
     5581 cggcgccgta gcaggtgcgt ggatagccgg cgccgccgaa cggccgctgg gcgatgcgcc
     5641 cgtcgtcgag gcggctgaag gggcagcccc agtggtccat ctcgaagatg acgcccgggg
     5701 cgtcgctggt catctggatg gcggcgtcct ggtcggccag gtagtcgctg cccttgacgg
     5761 tgtcgaaggc gtgcttctcg ggcgtgtcgt cgtgcgcgtc ggggtggttg gcgagcgccg
     5821 cgttgatgcc gccctgcgcg gcgctggagt ggctgcggcc ggggtggacc tggctgagga
     5881 ccgccacgtc cagtccggcc gccctggcct ccacggcggc gcggagcccc gcgagcccgc
     5941 cgccgaccac gatgagctga tggtgtggca tgcgctcccc ctcagcccac ccagacccag
     6001 agggcccagg tgccgtaggc gacggcgacg atgccggcga tccagaggac cgcgtcgaac
     6061 ccgcgccgca gaccggagcc ggtgagcccc cagtcgaggg ccaccatgcg gatgccgttc
     6121 agcgcgtggt agaccacggc gaccagcagc agcaggtcgt tgacgagcac ggcggtggac
     6181 gcgagccgct cgccgatgtc gtcgaagctg agctcgccga tggcgacgaa gtgcgtcagc
     6241 acgaggtgca ccatgatggt gacgatgatg acgaccgcgg tgacgcgctg gcccagccag
     6301 ggccacatcc cgccgcggct gcgatcgagt gtctcggttc tcactcccgc cctcctagaa
     6361 gatcaggggg atggccctgt aggcgaaccc ccacaccgcg agcgccgcga cgagcatgca
     6421 gacccagaac acggccttgt gccggtggat gccgatcccg acgtccatca gcaggatccg
     6481 cacgccgttg agggcgtggt agagcaccgc cgccaccagc acggagtcga ggaagacgag
     6541 gaacggcttg tcgaagaacg cgaagaagtc gtcgagtgtc tcggcgccgg acacgcgccc
     6601 ctgcgagatg acccagatgt ggacgaacag gtagacggcg aggacgaggc cgctgatgcg
     6661 gaagaggatc caggcccaca tgccgatgcc cttccacagg cccgagccga cgttggccgt
     6721 ctcgcgctcc gtagcgctca agatgagtac cccctcacag actcgcgccc cccatgtcgg
     6781 gcggcaggct ccccatggac cacgatcgta gaacgcgtcc ctcgtagcat aacaagaccg
     6841 gctcgtcagc ggcgccgctt gcgcccgccg cggcgggcgc gctgccgcgg cgccggccgc
     6901 cgcgccggcc gcgcgccggc gtccaccgcg gcgacgatgc gcgcggcgcg caggcgctcg
     6961 agcaccgcca gcacgaggtc ggcggccgtc ttggcgacaa ggaggacggc gaggccggtg
     7021 agcgacgagt cgaacagcca gtagacgagc agcgcgccgg ccaggacgcc gaactgcagc
     7081 acgaagaggc ggcggagcgg ctccgccacc gccgcctccg gagggatcac cgcagcctct
     7141 ccgccacgcg cgaagtccca ccagtagagg aacgcgcgca tggccacaag gcccccgacc
     7201 accgcccagt actgccaggt gacgaacgcg gcgccgggcg cgtccggcgc ctcacctccc
     7261 ggcttgagca gcatgccgct gaagaccatg tacgcgatga cggcctgagc gccgacgacg
     7321 atgaggtagg tgcgcacgaa ctcgcgcacc agcacggcgt cgcggtcgtc cgtcgccgcg
     7381 cccgccgcga acgaggcgcg ggcgcgcagc caggcgaaga tgccgtcggc caagccgtcg
     7441 atgacgcagg cgaccacgac caccagcgcc gtccacgagc ccgtcacggc ggcccagacg
     7501 accgccacgt cggccaccag gagcacggcg aagctgggca cggcgcgcgc cagggtcgcg
     7561 cccgtcggca caggggaagc cacggccgcc ccgcgtgcgt cgccggccgt ggccggcggc
     7621 cgcgggtcag tgccctggct ggtgtcgcgc tcgctcatgt gccccctcgc agccaccgcg
     7681 ccggcgaagc cggcgcggag tccgcacagc gtacgcggcg gcgccccgaa aggcaacgcg
     7741 cacgggtgat gatgcgatgg cctcgctcac cgcgccggcg aggcgacggt ggccgcaggc
     7801 ggcggggatc ggagcgtctt agcgcccgtc ccggggctgc agttcggtga agacgaccag
     7861 acgtttggcg tgcgtcccgg cggcgacgtc gaacgagccg gtgcaggtga tcaggttgag
     7921 gcggcggtgc ttcgtggacc cgaacacctc gccggcgtcg gcgttgcgcc cgaagagcac
     7981 ctttctgcgc acgacgaacg cgagccgctt gcccttcgcg tccttgacga acaccttgtc
     8041 gcctaccttc aacttcggca ggtcgtcgaa gaccgcctcc ctgtcgtcgg cgtaaccgct
     8101 gtggccgtcg atcacagcgc ttccctcgtt gccggggcgc ggcccgaact tgtaccagcc
     8161 gaccgtgtcg gcgcgagagg gcgacgccat gtcccggcgc gacgtcagac cgaccttctc
     8221 gacggcggcg ttgaccccga gcctggggat gatcaggcgc acgggaaggc catacgtgac
     8281 cttcgggacc gcggccgggg tcgccggctc ttgggccgga tccgtcaact ccgcagcgtt
     8341 ggcggcggga ctctcggtgg agccgtccgg cgcgacgatc agatagacgc cggcggccag
     8401 ggcggccgcg gcgacgacca caaggacgag cacggctgcc cgccgcgagg cggacagccg
     8461 tgctctcttg ttcttcactt gcaccccatc ctgcggatcg gcaggtggct ccgggggtgc
     8521 ttcctcagtt gtgccgacgt gcgggatgca gtctccaagc ggcgaacagc agggtcagac
     8581 cggccgcgaa cgcgccgctg accagcagcc atgaagtcga gccgccgtcg ctgggcggat
     8641 agcccgtggc ggggaagccc acgtcctcgg cgtcctcgtc ctcgtcagtc acgccaccgg
     8701 tgccgctccc atcgtccgta gcgctcggac tcggggtcgg ggtggccgtg tcggtgggcg
     8761 taggcgtcgg ggtcggggtc ggcgtgtcgg tgggcgtcgg cgtcggggtc ggcgtcggcg
     8821 tgtcggtggg cgtaggcgtg ggcgtcgggg tcggcgcagc atggcagccg gtgccgaagc
     8881 agccgccgcc ggccggatgg gaggccggaa gcgtgtcgtg acaactcgcg cacgctgcag
     8941 gcgtcggtgg cgcgtcagtg ccgttggtgt ggcaggtggc gcaacccacc gttgggtggg
     9001 cggcgtgcgt ggtggccaac gtgtggcaac tgc
//
LOCUS       DASUMX010000110         3265 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_2042796, whole genome shotgun sequence.
ACCESSION   DASUMX010000110 DASUMX010000000
VERSION     DASUMX010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3265
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2042796"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1212)
                     /gene="aroA"
                     /locus_tag="VFZ86_05455"
     CDS             complement(<1..1212)
                     /gene="aroA"
                     /locus_tag="VFZ86_05455"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEX5641773.1"
                     /translation="MNAARSSSPRSIRPARPGLDARVRVPGSKSVTNRALLLAGLAAG
                     ESVLHGALTAGDTEAFAAGLRSLGVAVERRGDDLHVSGAGGRFPAARAEVFCSEAGTA
                     ARFLLAAAAAGEGVYRFDAAPQLRRRPLGLLLDALRSQGARTDPDGADSLPLTLLAGG
                     LAGGSVRLPGDTSSQFVSALLMAAPLGRTPLDLRVDGLVSRPYVTMSLRMMDAFGAGP
                     ASAAGDRFVVAPGSYAGRVYAVEPDASTASYFFAAAAVAGRVQVLGLHRAHALQGDVA
                     FLDVLECMGCVVDDAPEGVRVAGPAALAGLTVDMADIPDTFMTLAAIAPLATSPVTVT
                     GIGNVRLKESDRIAAMEDNLRRTGVRTESGADWLRVFPCTPRGASVDPRGDHRIAMAF
                     SVLGLRVPGIVI"
     gene            complement(1235..1588)
                     /locus_tag="VFZ86_05460"
     CDS             complement(1235..1588)
                     /locus_tag="VFZ86_05460"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX5641774.1"
                     /translation="MGRLIMVLTVAAVLAAGAVACGGSSTDASTTAPAPAETQVVPTE
                     TAGEVAALFADDCAGCHGADGGGGSGPDIRGEDSLDDVKAQIESGGGGMPAFSGQLSP
                     GQIDALAQYVTGELR"
     gene            complement(1702..2634)
                     /locus_tag="VFZ86_05465"
     CDS             complement(1702..2634)
                     /locus_tag="VFZ86_05465"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5641775.1"
                     /translation="MTPSRVRPATRGDIPEIVDLLCACDVAEIGAPDSTVDDLENDWA
                     MEGFDPSRDAWVAEGPAGLLGYAYAGDQFRTGELEADFWTHPDHHEPELGVRLLGLCE
                     RRAAQLAVERGYTDARLDVFCSTANRAKRDLLRRHGYALERTVYRMTTDLSEGAPPTE
                     APEGLEILPFRLEADERALYELMNEAFVDHFRQSQEPFDAWQARLLGHADFDPELWWI
                     AWDGGQAAGGLIAYDHGDLGWIKGLGVRRPWRRRGLGTALLGHALAAFCARGQLRVEL
                     GVDAEGATAPLRLYERAGMQATSAYELYTKPLAG"
     gene            complement(2634..>3265)
                     /locus_tag="VFZ86_05470"
     CDS             complement(2634..>3265)
                     /locus_tag="VFZ86_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641776.1"
                     /translation="AGAAGASTGGPAYGAPAAYGGPAYGAPPAYGGPGEWGPEYQPQP
                     AAAPAPASAGASGLLKLVLVGGAVAIVAVLLVWFFVLRGPATTGEEFLGSWTASTQQG
                     IATVVVAQEAEAFSVTMSGNDQNQKVTVPAHLDGTELVITMDDFSQMAGAGNAEAFKA
                     TLKALAGDFTMRFSSVDATHLGLRIVGESPSGQDFDETIPLSRDPAGTT"
BASE COUNT          424 a         1191 c         1246 g          404 t
ORIGIN      
        1 gatcacgata ccggggacgc gcagccccag caccgagaag gccatggcga tgcggtggtc
       61 gccgcgcggg tccacggacg cgccccgggg cgtgcagggg aagacccgca gccagtcggc
      121 tccggactcc gtcctcaccc cggtccggcg caggttgtcc tccatggcgg cgatgcggtc
      181 ggactccttg aggcgcacgt tgccgatgcc agtgaccgtg accggcgacg tggccagcgg
      241 ggcgatggcg gccagcgtca tgaaggtgtc cgggatgtcg gccatgtcga cggtgaggcc
      301 ggccaacgcg gccgggccgg ccacacggac gccctcgggc gcgtcgtcca ccacgcaacc
      361 catgcactcg agcacgtcga ggaacgcgac gtcgccctgc agggcgtgcg cgcggtgcag
      421 gccgagcacc tggacgcgcc ccgcgaccgc cgcggcggcg aagaagtagg acgccgtgga
      481 ggcgtcgggc tccactgcgt acacgcgccc cgcgtagctc cccggggcga cgacgaaccg
      541 gtcgccggcg gcggacgccg gtcccgcccc gaacgcgtcc atcatccgca ggctcatggt
      601 gacgtacggg cggctcacga gcccgtccac gcgcaggtcg agcggcgtgc ggccgagcgg
      661 cgccgccatg agcagcgcgg agacgaactg gctggaggtg tcgccgggca gccgcaccga
      721 gccgccggcg aggcctccgg ccagcagcgt gagcggcagg gaatcggcgc cgtccggatc
      781 ggtccgtgcg ccctgggagc gcagcgcgtc gaggaggagg ccgagcggcc ggcgccgcaa
      841 ctgcggcgcc gcgtcgaagc ggtagacgcc ttccccggct gcggcggcgg cgagcaggaa
      901 ccgggcggcc gtgccggcct cggaacagaa cacctcggcg cgggcggccg ggaagcgtcc
      961 ccccgcgccg ctcacgtgca ggtcgtcgcc ccgccgttcc acggcgaccc ccagactgcg
     1021 caggccggcg gcgaacgcct ccgtgtcgcc ggccgtgagc gcgccgtgca ggacagactc
     1081 gcccgcggcg aggcccgcga ggagaagcgc ccggttggtc acgcttttcg agcccgggac
     1141 gcgcacgcgc gcgtcgagac cgggccgtgc ggggcggatg ctgcgagggc tgctggagcg
     1201 ggcggcgttc atctcccgca tgataccgca gggcctaccg cagctcgccg gtcacgtact
     1261 gcgccagggc gtcgatctgg cccggcgaca gctggccgga gaaggccggc atcccgccgc
     1321 cgccactctc gatctgggcc ttgacgtcat ccaggctgtc ctcgccgcgg atgtccggac
     1381 cgctcccgcc gccgccgtcg gcgccgtggc agccggcgca gtcgtcggcg aacagcgccg
     1441 cgacctcgcc ggcggtctcg gtggggacga cctgggtctc ggcgggcgcg ggcgccgtcg
     1501 tgctcgcgtc cgtggacgag ccgccgcagg cgacggcgcc ggcggcgagg accgcggcaa
     1561 cggtgagcac catgatcaag cgacccatac gggctccttt ggtaggggcg tggacggcat
     1621 cgtcattccc gtcccgccga tgcggcaacc gtcggccggg acgccggccg tcggccggcg
     1681 gggcggcccg cagcgaccgg atcagccggc cagaggcttg gtgtacagct cgtaggccga
     1741 ggtcgcctgc atgccggcgc gctcgtacag ccgcagcggc gcggtggcgc cctcggcgtc
     1801 cacgccgagc tccacgcgca gctgcccgcg cgcgcagaac gcagcgagtg cgtgccccag
     1861 cagggcggtc cccagtcccc ggcgccgcca gggccggcgc acacccagcc ccttgatcca
     1921 gccgaggtcg ccgtggtcgt aggcgatgag accgccggcc gcctggccgc cgtcccaggc
     1981 gatccaccag agctccgggt cgaagtcggc gtgaccgagc aggcgggcct gccaggcgtc
     2041 gaagggctcc tggctctggc ggaagtggtc cacgaacgct tcgttcatga gctcgtagag
     2101 cgcgcgctcg tcggcctcga gccggaaggg caggatctcg aggccctccg gcgcctcggt
     2161 gggcggggcg ccctccgaga ggtcggtggt catccggtag acggtgcgct cgagcgcgta
     2221 gccgtgccgg cgcaggaggt cgcgtttcgc gcggttcgcg gtgctgcaga agacgtcgag
     2281 cctggcgtcg gtgtagccgc gctccaccgc gagctgtgct gcccgccgct cgcagaggcc
     2341 gagcaggcgg acgccgagct ccggctcgtg gtggtcgggg tgcgtccaga agtccgcctc
     2401 gagctcgccg gtgcggaact ggtcgccggc gtaggcgtag cccaggagtc cggcgggacc
     2461 ctccgccacc caggcgtccc gcgaggggtc gaatccctcc atcgcccagt cgttctcgag
     2521 gtcgtcgacg gtgctgtcgg gcgcgccgat ctcggcgacg tcgcacgcgc agagaaggtc
     2581 gacgatctcg gggatgtccc cccgcgtcgc cgggcggaca cggctcgggg tcatcatgtc
     2641 gtgccggctg ggtcccggct gagggggatg gtctcgtcga agtcctgccc cgacggcgac
     2701 tcgcccacga tgcgcaggcc gaggtgggtg gcgtcgacgc tgctgaaacg catcgtgaag
     2761 tcaccggcga gcgccttcag cgtcgccttg aacgcctcgg cgttgcccgc gcctgccatc
     2821 tgcgagaagt cgtccatggt gatgaccagt tccgtgccgt cgaggtgcgc cgggacggtg
     2881 accttctggt tctgatcgtt cccgctcatg gtgaccgaga acgcctccgc ctcctgcgcc
     2941 acgaccacgg tggcgatgcc ctgctgagtg ctggcggtcc agctgccgag gaactcctcg
     3001 ccggtcgtcg ccgggccgcg caggacgaag aaccagacca ggaggacggc gacgatcgcg
     3061 acggcgccgc cgacgagcac cagcttgagc aggccggagg cgccggcgct cgcgggcgcc
     3121 ggcgcggccg cgggctgcgg ctggtactcc ggaccccact cgcccgggcc gccgtacgcc
     3181 ggcggcgctc cgtaggccgg gccgccgtac gccgccggcg ctccgtaggc cgggccgccg
     3241 gtcgaggcgc cggccgctcc ggccg
//
LOCUS       DASUMX010000111         2316 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1755344, whole genome shotgun sequence.
ACCESSION   DASUMX010000111 DASUMX010000000
VERSION     DASUMX010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1755344"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="VFZ86_05475"
     CDS             <1..187
                     /locus_tag="VFZ86_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641777.1"
                     /translation="EAANAMYIIHLMGVVPMLVLEVPFGKWAHLAYRPLAIYFSRVQV
                     HAEESEAARAQKAAQAA"
     gene            complement(365..1030)
                     /locus_tag="VFZ86_05480"
     CDS             complement(365..1030)
                     /locus_tag="VFZ86_05480"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX5641778.1"
                     /translation="MDAARPHPTPTTRAGFEAVVRDPCHVRHFRDDAVPREDVEHMVD
                     LAMCAASTCSAQAWRFLAVQDQGLIAAMRAAVLERFEELALTPGLALQEHKRTAARAQ
                     ALLFAKAPLCIAVLALPSGSPIEELMELAGMTQEEHDRLCVRPELQSAGAAVQLLTTS
                     AHSLGYAACWTCAPIVAGERLERLLQVEAPARLVALVAIGRPAEQPTSTRRLPLERAL
                     SFR"
     gene            complement(1267..1629)
                     /locus_tag="VFZ86_05485"
     CDS             complement(1267..1629)
                     /locus_tag="VFZ86_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEX5641779.1"
                     /translation="MSEFDVNEAIRRIKADPGAAGVGMILAHQGIVRGSSRAGERVHG
                     MHLKVDRGRFDQALAEARDWPGIVAVDGWVNEGDLKVGDDIMKVVVAGDIRENVFGGL
                     QRLVGIIKSEVVTESEFR"
     gene            1657..1881
                     /locus_tag="VFZ86_05490"
     CDS             1657..1881
                     /locus_tag="VFZ86_05490"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmO family methyltransferase"
                     /protein_id="HEX5641780.1"
                     /translation="MNSNPIGLSVVRLVERRGRELLVEDLDVLEGTPLLDIKPYVEGF
                     DLRVGTRGGRTEQVGSDEFLRRGERRPDVR"
     gene            complement(1891..>2316)
                     /locus_tag="VFZ86_05495"
     CDS             complement(1891..>2316)
                     /locus_tag="VFZ86_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1847 domain-containing protein"
                     /protein_id="HEX5641781.1"
                     /translation="GFARLGVAHCIGLQDEAAVVKRVFEANGFAVDAVACKTSALPKE
                     VLGLMDADKVSPGEFESMCNPIGQARALDHTGTELNVLLGLCVGHDSLFMKHSVAPVT
                     VLAAKDRVLGHNPLAAIYLADGYYCERLFPARQGSETAG"
BASE COUNT          360 a          811 c          787 g          358 t
ORIGIN      
        1 cgaggcggcc aacgccatgt acatcatcca cctcatgggc gtggtgccga tgctcgtgct
       61 cgaggtgccg ttcggcaagt gggcgcacct cgcgtaccgg ccgctcgcca tctacttcag
      121 ccgcgtgcag gtgcacgccg aagagtcgga ggcggccagg gcacagaagg ccgcacaggc
      181 ggcctgatcc ggaggaaggc cgcgccgacg gcgcggcctc agccggcggc tccacccggg
      241 atggcgccgc cgggccgggc gcccaacgaa cggcccgcga tcgtcggaga cgacggtcgc
      301 gggccgttct ctcgttcgtc aggcggtcgg ccgtgtacga ggcaaagaag ccggcgtcga
      361 gccgtcagcg aaaggagagt gcgcgctcca gtggcagccg tctcgtggag gtcggctgtt
      421 ccgccggccg cccgatggcg acgagcgcca ctagccgggc cggtgcttct acctgcagca
      481 gtcgctccag ccgttcaccg gccacgatcg gagcacaggt ccagcaggcc gcgtacccca
      541 gggagtgggc ggacgtggtg agcagctgga cggcggctcc cgcgctttgg agctccggtc
      601 gcacgcacag gcggtcgtgc tcctcctgcg tcattcccgc cagctccatc agttcctcga
      661 tcggcgaacc ggagggcagg gccagcaccg cgatgcagag tggagccttg gcgaacagca
      721 acgcctgcgc acgagccgcc gtgcgcttgt gctcctgcag cgccaggccg ggagtgagag
      781 cgagttcctc gaaacgctcg aggacggcgg ctcgcatcgc agcgatgagt ccctggtcct
      841 ggacggcaag gaaacgccag gcctgcgcgc tgcaggtgct ggccgcgcac atcgcgaggt
      901 cgaccatgtg ttcgacgtcc tcgcgtggca ccgcgtcgtc ccggaagtgc cgcacgtgac
      961 atgggtcgcg caccaccgcc tcaaagcctg cgcgcgtggt cggggtgggg tggggcctgg
     1021 ctgcgtccat gactgcctcc ggtctgccca caacgaccgt gatcactgct ccatggaggc
     1081 gttcgtcctc cacacacggc ccgctgtagt tagcatatgc tcagaatata cgcccacgcg
     1141 cgggtgtcaa gatggaggac cgtgggctgg cctcgtgagc atatgctcat cgaggatcgc
     1201 aggcttggag gcagctgcgc cgccgcggca aggacgtcgt cgccgtaggg gcaggtcgcg
     1261 gcagagtcag cggaactcgg actccgtcac gacttcgctc ttgatgatgc cgacgaggcg
     1321 ctgcaggccg ccgaagacgt tctcgcggat gtccccggcc acgacgacct tcatgatgtc
     1381 gtcgcctacc ttgaggtctc cctcgttgac ccagccatcc acggcgacga tcccgggcca
     1441 gtcccgagcc tcggcgagcg cctggtcaaa tcgcccccgg tcgaccttca ggtgcatgcc
     1501 gtgcacccgc tcaccggccc ttgacgagcc gcggacgatg ccctggtggg caagaatcat
     1561 cccgacgccg gccgccccgg ggtcggcctt gatccgccgt atggcctcgt tgacgtcgaa
     1621 ctcgctcatg gctccctcgc aggtcgactg cctcgagtga atagtaaccc catcggcctg
     1681 agcgtcgtcc gtctcgtgga gcggcgcggc cgcgagctcc tcgtcgagga tctcgacgtc
     1741 ctggaaggca caccgctcct ggacatcaag ccgtacgtcg aagggttcga cctccgcgtc
     1801 ggcacccgcg gcggccggac ggagcaggtg ggcagcgatg agttcctgcg gcgcggagag
     1861 cgtcggccgg acgttcgtta ggggctccgc tcacccggcg gtctcggatc cctgccgcgc
     1921 cgggaaaagc cgctcacagt agtacccgtc ggccaggtag atggccgcca gcggattgtg
     1981 cccgagcacc cggtccttgg ccgcgagcac ggtgaccggg gccaccgagt gcttcatgaa
     2041 caggctgtcg tgcccgacgc acaacccgag cagcacgttc agctccgtgc ccgtatggtc
     2101 cagcgcccgc gcctggccga tggggttgca catgctctcg aactccccgg gggagacctt
     2161 gtccgcatcc atcaggccga gcacctcttt cggcagcgcc gaggtcttgc aggcgaccgc
     2221 gtcgaccgcg aagccgttgg cctcgaacac ccgcttgacc accgcggcct cgtcctgcag
     2281 cccgatgcaa tgcgcgacgc ccaggcgcgc gaatcc
//
LOCUS       DASUMX010000112         2251 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_317828, whole genome shotgun sequence.
ACCESSION   DASUMX010000112 DASUMX010000000
VERSION     DASUMX010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317828"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..1283
                     /gene="tyrS"
                     /locus_tag="VFZ86_05500"
     CDS             51..1283
                     /gene="tyrS"
                     /locus_tag="VFZ86_05500"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEX5641782.1"
                     /translation="MADSRLLDDLMIGAVDVLPEGALEAKLDLAEREGRPLRVKLGVD
                     PTAPDIHLGHTVVLNKLRQFQDAGHIAVLIIGDYTARIGDPSGRSKTRPRLDGAIIDA
                     NAETYKEQAFLILDADPAKLEMPYNSEWLAPLTMEQLFEITATTTVARILERDDFANR
                     WAAHQPISMLEMLYPFAQGYDSVAVEADIELGGTDQVFNLFMGRDLQTYFGQEPQAIM
                     TMDILPGTDGVERMSKSVGNYIGVTEDPRDMYGKVMSIPDDVMPTYFRLLTPHHADEL
                     ARIEQDYQSGAVNPRDLKARLAREIITRLRGAAAAAAAEEHFDSVFRRHESAAGLQEL
                     ALEAGDVEEDGKVFLPAVLERWLGQTRSEWRRRIQQGGVSLDGEPVSDLAVPAAALAG
                     RRLKAGKSARAQGVIRGI"
     gene            1868..>2251
                     /locus_tag="VFZ86_05505"
     rRNA            1868..>2251
                     /locus_tag="VFZ86_05505"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          408 a          760 c          732 g          351 t
ORIGIN      
        1 cctccggccg gcccgtccga gtcgccgtac ccgttcgaaa ggaccgagcc gtggccgaca
       61 gccgcctcct ggacgacctc atgatcggcg ccgtcgacgt gctgcccgag ggcgcgctcg
      121 aggccaagct cgacctcgcc gagcgcgagg gccgcccgct gcgcgtcaag ctcggcgtcg
      181 accccacggc gccagacatc cacctgggcc acacggtggt cctcaacaag ctccgccagt
      241 tccaggatgc cggtcacatc gccgtgctca tcatcggcga ctacacggcg cgcatcggcg
      301 acccgtcggg gcgcagcaag acgcgcccgc gccttgacgg agcgatcatc gacgccaacg
      361 ccgagaccta caaggagcag gcgttcctca tcctcgacgc tgacccggcg aagctcgaga
      421 tgccgtacaa cagcgagtgg ctcgcgccgc tcaccatgga gcagctcttc gagatcaccg
      481 ccacgaccac cgtggcccgc atcctcgaac gcgacgactt cgccaaccgc tgggccgctc
      541 atcagcccat cagcatgctg gagatgctgt atcccttcgc ccagggctac gactccgtcg
      601 ccgtcgaggc cgacatcgag ctcggcggca ccgaccaggt cttcaacctc ttcatgggtc
      661 gcgacctgca gacgtacttc ggccaggagc cgcaggcgat catgaccatg gacatcctgc
      721 cgggcaccga cggcgtggag cgcatgagca agagcgtcgg caactacatc ggcgtgaccg
      781 aggacccccg ggacatgtac ggcaaggtca tgagcatccc cgacgacgtg atgccgacgt
      841 acttcagact gctgacgccg caccacgccg acgagctggc gcggatcgag caggactacc
      901 agagcggcgc cgtcaacccg cgtgacctca aggcgcgcct cgcgcgcgag atcatcacgc
      961 ggctgcgcgg cgccgcggcc gcggccgcgg cggaggagca cttcgacagc gtcttccgcc
     1021 gccacgagtc cgccgccggc ctgcaggagc tggcgctcga ggccggcgac gtggaggagg
     1081 acggcaaagt gttcctgccg gccgtcctcg agcgctggct cggccagacc cgcagcgagt
     1141 ggcgacggcg catccagcag ggcggcgtga gcctggacgg cgagccggtc tccgacctcg
     1201 ccgtccccgc cgccgccctc gccggccggc gcctcaaggc cggcaagtcc gcgcgggcgc
     1261 agggcgtgat ccgcggcatc tgaggagcgt tctctcccgc tccgtcaggc gtgcggcgag
     1321 tctccgggag cccggcgcaa agaatttgcc caagtcaggt tgacaccgtc ctttcacggg
     1381 gtatactccc tgagcgcttg aggagtccgc tcgttgcaga cagtgtctcg atgatgacca
     1441 agagcgcgaa tcgcttgaac ggctcggccg cttcctgtac actaggaagt ccctgccggg
     1501 acgtgtggtg aggcaagcca aaccccgcga tcacctctgc agggttcgtg cgcgctgaaa
     1561 ccgctcgcga ttcaccaagt gcccacgggc acgaggtctt tgaaaactga acaggatgag
     1621 gaagccgatc agccggcgtc gcccttgtgg cggcacgatg atcggtggac gccagtgcgc
     1681 ctggcacgcc ttcgggtgtg cctggttcaa atgatttatc ccgtcagtga ccctgcattg
     1741 ctgaaaccgg cggcccttgg gccggcggac cgggggtcac agacaaccga ggcattcgtg
     1801 cttcggcttt tgagctttcg agctctctct aagcttgtcc cgcgccttcg ggcgcgagca
     1861 ccaagttttc acggagagtt tgatcctggc tcaggacgaa cgctggcggc gtgcttaaca
     1921 catgcaagtc gaacgagaaa gcgccttcgg gcgtgagtac agtggcgaac gggtgagtaa
     1981 cacgtgggca acctgccctg gagactggga taacgttccg aaagggacgc taataccgga
     2041 tgacgcgcga tctgccaagt aggtcgcgcc aaatggtagc ttcggcttct actctgggat
     2101 gggcccgcgc cggattagct tgttggtgag gtaacggctc accaaggcga cgatccgtag
     2161 ctggtctgag aggacgatca gccacactgg gactgagaca cggcccagac tcctacggga
     2221 ggcagcagtg gggaatcttg cgcaatgggc g
//
LOCUS       DASUMX010000113        31875 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1611503, whole genome shotgun sequence.
ACCESSION   DASUMX010000113 DASUMX010000000
VERSION     DASUMX010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 31875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31875
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1611503"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1919)
                     /locus_tag="VFZ86_05510"
     CDS             complement(<1..1919)
                     /locus_tag="VFZ86_05510"
                     /inference="COORDINATES: protein motif:HMM:NF025925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HEX5641783.1"
                     /translation="MSETPATRPRTAMATITFTPSGETIEVPQGTSLLDAAILCELDV
                     PAPCAGQGRCGRCRVRITSGEVERRSNAGLATAEILDGWAVACQTYVSGSVSVEVPER
                     RKEKVRPHGHAVAEPESLPITCDYLQNPAVRLFELVIEPPSLADQTSDFDRLRRALQQ
                     QHGITEVRAELPQLRRLGTALRHANWKVTVALEMRDWVYGSYLPPRLLRVYPTAFPQA
                     SMGLAVDLGTTSLVAYLVDFTTGRVVDTASAYNKQISCGEDVISRIIYAKRKHGLERL
                     QKLVIETINDLLDELQQRNGAEMREIHEVAIAGNTTMTHLLLGVEPRYLREEPYIPTV
                     SSAPKLVAHELGLNANPVARVHVLPSVGAYVGGDITAGVISSGLYATDKLTLFIDIGT
                     NGEMVLGTKDWLLSCACSAGPAFEGGGVRDGMRASPGAIEDVYIDDATLEPTFRTIDD
                     APAVGICGSGLIDLLGELFVTGLLDKSGHLDQQAQTARARVRDGVPEYVVCRAGEGGA
                     EKDIVLTESDITSLIRAKAAIYAGFEVLCASVGVDLADIEQVLIGGAFGQYLNVEKAI
                     RIGLLPDQPVERFHFLGNTSALGAFATLLCVNVRREVADVASKMTYLELSADNTFMDE
                     YTSALFLPHTDLTGFP"
     gene            complement(1916..3265)
                     /gene="acsC"
                     /locus_tag="VFZ86_05515"
     CDS             complement(1916..3265)
                     /gene="acsC"
                     /locus_tag="VFZ86_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006521917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit gamma"
                     /protein_id="HEX5641784.1"
                     /translation="MALSGLQIYKLLPQTNCKECGFPTCLAFAMKLAQKGTELDKCPY
                     VSDEAKTALDAASAPPIKLATIGAGDRAFAVGNETVMYRHEKTFVHQPGLVVRVKSDA
                     ADLGDVAARVAAYSVERVGMTLTLDGLAVEDAGGGADAYAAAVAAARAAAPGLPLVLM
                     SVDAAAVDAALAGGAAAEKPLIHAAIAANWQAMAEAAKKHGCPLVIKVTDGDLDGLLE
                     LSGQVRGAGVEDLVLDPGATTLAGDLAAATQLRRLALKKGARELGYPLVAVAAAPTWQ
                     AELVRATQAVGKYAGVIIVDHFEPAMLYTLLTLRQNIYTDPQKPIQMEPKLYEIGAVT
                     ADSPVLITTNFSLTYFSVAGEVEGAGIPTWLLVADSDGQSVLTGWAAGKFDAEKIAKT
                     VKISGIEQKVVHKKLVIPGHVAGLSGEVEEELPDWKIMVGPRDAVDIPNYIKNVWGA"
     gene            complement(3269..4234)
                     /locus_tag="VFZ86_05520"
     CDS             complement(3269..4234)
                     /locus_tag="VFZ86_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit delta"
                     /protein_id="HEX5641785.1"
                     /translation="MTPVEIPVEKWSGAVREVTLGSSYGGGTRGSTVTVGGETALPFL
                     GFEGAMPHQPAIAVEVQDMHPADWSAELQAAWGDAMDDAATWAQKAVEAGADLIALKL
                     RSCHPDQGDGSCEDAVRTVERVLAAVPVPLIVYGPGSADKDNEVLVAVAEKTKGEQLA
                     LGLCEDKNYRTIVAAALGNGHLVIAQTPIDVNLAKQLNILISDMGLPLERVLMDPNTG
                     ALGYGLEYTYSVMERLRLAALMGDSMTQQPMICTAGEEAWRQKEGRAEGDVPEAWGAL
                     AERGVAWEISTATTLLESGADILVLRHPASVSAVREALDDLTKGA"
     gene            complement(4266..6551)
                     /gene="acsB"
                     /locus_tag="VFZ86_05525"
     CDS             complement(4266..6551)
                     /gene="acsB"
                     /locus_tag="VFZ86_05525"
                     /EC_number="2.3.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit alpha/beta"
                     /protein_id="HEX5641786.1"
                     /translation="MSRYIATRAIRGAQGVVREAEEAVNAALAELGPETPVQFTNTAY
                     YLPVIYAFTGQKVEKLGDLPPVIERAKSLLHPIPGDQMWLPYLGETLDSGIATLFAEE
                     AIEGVRFARGSEPQVIKLEQAREDKARFTGGDLGVHGDKMKLNGPIDDIQLRAWGIQL
                     VDGRMPGFCAIVGCARSNEVAVKIVRELQSRGILIFLSGNVNGRSIIHQLQEEGIQLG
                     YDTFTVPFGIDTISAVYALGFAARSALTFGGMDAGNARKIMLYNKYRVFAFVLALGEV
                     DDLKYATAAGAISFGFPTIADTRIPQILPTGVTTYEHVVSMPFDEIPGADDMERTEKL
                     IQRCMEVRGVKVKIAKVPIPVSYGSAFEGERIRKDIMYAEFGGKRSNAFEFLRMKPTD
                     EVEDGKITVSGTDIDGMEEGGALPLGMVVDVSGRKMQTDFEPILERQFHYFINGVEGI
                     QHNGQRDIAWTRVSKSTFGKGFRLEHFGKVIHARIHEVFGNIVDKVQVHLTTDQAEVD
                     ELIEVARVAYAERNERIANLTDESVDTFYSCLLCQSFAPNHVCVINPERVGLCGAYNW
                     LDCKASYEINPTGPNQPVAKGLVIDAEKGEFSGPNEYIYQNSNQSVERVTLYSIMEAP
                     LTSCGCFEAIMVLVPEANGFMIVSREDPSMTPCGMTFSTLAGTAGGGMQTPGMMGMGK
                     YYLTSRKFISADGGMKRIVWLSSNLRESMRPALQEVCEREGVPDLLDKIADETVATTV
                     EDLLPFLEEKGHPALTMDPLF"
     gene            complement(6551..8650)
                     /gene="cooS"
                     /locus_tag="VFZ86_05530"
     CDS             complement(6551..8650)
                     /gene="cooS"
                     /locus_tag="VFZ86_05530"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018492 - carbon-monoxide dehydrogenase
                     (acceptor) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HEX5641787.1"
                     /translation="MAEKINDDVKPVAAKSIDPAALEMLAHAEGCGLETAFSRADTMK
                     PCPIGESGACCRICSMGPCRLVGKDAEQKTGICGADLPTIAARHFARQVAGGVSAHSD
                     HGRDMAMTLRAVAMGEAAGYKIKDEQKLHSVAGYFGIPTSGREVNEIALDVADVALAQ
                     FGQPHGEIVYTRRATPRRQARWRELGLTPRAIDREVVEVLHRTHEGVDLDAENILKSA
                     LRCSLADGWGGAMLSTDISDILFGTPSPLSSEVNLGVLKDDQVNVIVHGHEPTLSAML
                     VEAARDPELVAEARAAGAEGINLAGICCTSNEILMRYGIPAAGNFLHQELAVLTGAVE
                     TMVVDVQCIMQALGPLAQRFHTQLVTTSPKAKIPGATHVEFDEHRAPETAREIVRMAI
                     ANYPNRGPVHIPDFHEPLVAGFSHEYLNYMQGGFHRGSFRPLNDAIIAGRIRGLAGVV
                     GCNNVRVTQDEGIVEMIKRFIADDVMVVVTGCAATAGAKAGYLSPEILDHAGPGLREV
                     MEAIGIPPVLHLGSCVDNSRILTVLTQVATEGGLGEDIDDLPAVGLCPEWMCEKAIAI
                     GTYFAASGAHVIFGVSSPVEASSVATRLMTEGWEELVGGDLEFIPDWDAMYARSLELI
                     DAKRAALKLPQYDPSQYGDSGDSLFFAEIKRYLADKAAEAERGVETVGSPVYPLAEGH
                     THDHGDGHTHTHDEGEA"
     gene            complement(8755..9078)
                     /locus_tag="VFZ86_05535"
     CDS             complement(8755..9078)
                     /locus_tag="VFZ86_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEX5641788.1"
                     /translation="MEQLALSVPGMWADHHVIAVRGLFQQAEDVQVTGASAMDATLRL
                     EYDPAKTDPQRIVAQLEQAGYAAGETPEADAPPTDKPAWLTAGVRVTTTDPADLTMSG
                     DHRKY"
     gene            complement(9355..9759)
                     /locus_tag="VFZ86_05540"
     CDS             complement(9355..9759)
                     /locus_tag="VFZ86_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641789.1"
                     /translation="MKRTRIAIITIQLLVMLNAFGGGYYGLSGAPGVDPAWLDGTPFS
                     SYFVPSLFLFTVIGGGMAVATAAWILKSRVAPWLSLAMGVTLMSWIVVQVAMIGYVSW
                     MQPVSFVAGAAVAALAGSALRGVRRERLDVPA"
     gene            9943..10650
                     /locus_tag="VFZ86_05545"
     CDS             9943..10650
                     /locus_tag="VFZ86_05545"
                     /inference="COORDINATES: protein motif:HMM:NF014906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX5641790.1"
                     /translation="MTAEVVVDAALAVIDAEGLDALTMRRLAHDLGVEPVTIYRQLPN
                     KDAILAGVAEKLWREMAPPDAGDQGEAPRAPAGADAPDWRAQVRGMWLALNELMQRHP
                     NAIPLIARGGSYSPSAGEGTAGMLRIFKDAGLTPEEAAELLHILSACVVGFGFASLWV
                     RQIAAARKAAPAGDELEPTRPPDEAGDLADYLEATRRWDPAQFTAAVDIVLRAYGDDA
                     KDRAAAAPHRGRPTTAA"
     gene            complement(10637..10837)
                     /locus_tag="VFZ86_05550"
     CDS             complement(10637..10837)
                     /locus_tag="VFZ86_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641791.1"
                     /translation="MLCSLATKLDQGQIEEITSLEGELGVPILAFACHQMDAAPIDAA
                     QIDRLKQLEDRLGVALVAVKQQ"
     gene            complement(11047..13290)
                     /locus_tag="VFZ86_05555"
     CDS             complement(11047..13290)
                     /locus_tag="VFZ86_05555"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX5641792.1"
                     /translation="MTDAPPTGAPQITIDGRVLAFEPGQTILDVANGGGIAIPTLCYL
                     PEAGHRDVCRLCVVEVAGAGRLLPACSTPATEGMDVRTMSDRVRHSRRATLELLINSG
                     RHTCITCDALGECELSRLAYEYQVEPPSELPGAEEFPLVEDAYVIRDYSKCILCGRCW
                     AACTAIQVHGVVPHPSGRRAERAGGKDWYPLPDLDQCELCGQCVDACPVGALTERTAK
                     GVARHWELQRIRTTCPHCGMGCQTVVHLKDGEVVKVTGAGDVPPNRGRLCKRGRFAVL
                     EPDERARLEDPLVRRNGVLVKAGWDEALDLVAERLGGVGAGGVAGLIAPVCTNEEAYQ
                     AQKFFRAVLGTNDIDHRAIEASRVSLAATGTGAGVCYTSGALTVLEEARAVLVVGDGA
                     VADHPVAGTALRRAVRDGAHLMVLDSGHSDLGDAAAVRLAVEPAAIPEILNGIIAVLL
                     ANELAGRRDAGEALPHERELEAMLAAVLPERIADDAGVDAVTIREIAAKLVDVRPAVV
                     CATLGTTDDLAGTGAAVVKLQSLLDDLAGARSVSLPLGAGNVQGLLAMGVAPDVLPGG
                     DLVTDAEARQRLADAWGVEAIPDAPGRSTAGIVEALAGGELKAAWVWANEAASFGDAE
                     ALLAALGKADFVVVQGAMRGDLCAVADVVLPAVAWGEEDGTFTNAERRISRLRRVQRP
                     AGAARPGWWTFREVARRLGHEWQAAYPKAVWEGEISLLIPSLGGVTYAEIDEYGLCWP
                     APAPVTA"
     gene            complement(13292..15178)
                     /locus_tag="VFZ86_05560"
     CDS             complement(13292..15178)
                     /locus_tag="VFZ86_05560"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5641793.1"
                     /translation="MTDAAVAVAGAWTTDPSRLPDELAGTFRAVMGWGGVAVWDESVN
                     IVDLTREFAARTVQECCGQCFPCRIGMHEVADILEEICSGEGTEARLERALELSRHVA
                     EASRCDLGRTGGYAVLESLEQHYDRFAAAVAAKESIPRGVYASTVVAPCMAACPAGVD
                     IPAYVEQLRIDEVERSADAVRRRCPMPGTIGRVCVRPCEDACRRGSVDEPVAIRTLKR
                     FVADSEPEPIACPMPGKPRVAIIGAGPAGLACAYYLGLDGIISTIFEALPEAGGMAAV
                     GIPDYRLPRDVLRAEVERVESLGVKIEYNFRFGVDATMDDLFAQGYEAVFIAVGAHQS
                     ARMGCEGEDAGYDGFMPGVEFLREIAFGKTPLTGKTMLVIGGGNVAMDCVRSARRIGF
                     DDVKLLYRRTEAEMPADRVEIVEAKEEGVEMVTLVAPTKIMAEDGKVVGLECRRMQLG
                     EPDSSGRRRPEPVEGSEFVIPCDCIVPAIGQVCSVDQLMPSHIEVSKWKTLEVDPLTM
                     QSADPRVFGGGDCYTGPSSLVAALAAGRRAAVSIDALLRGCDVAPALEERLEHAMLAV
                     IRDEAQAPIPFTGSTEKLRPMVVPPEVRIQCFDEVEGAVSAADARHEAERCLRCYRIA
                     VAVV"
     gene            complement(15395..16003)
                     /locus_tag="VFZ86_05565"
                     /pseudo
     CDS             complement(15395..16003)
                     /locus_tag="VFZ86_05565"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
     gene            16210..16635
                     /locus_tag="VFZ86_05570"
     CDS             16210..16635
                     /locus_tag="VFZ86_05570"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX5641794.1"
                     /translation="MPSELSVHAVQHGPMRFAISDGDHEVTVDYPLPGGDGDLGGMTP
                     LRLLLASLAGCSGSAVAALLRRDGQPVERVEVTARGRRRDEHPTVITDIDLAFVVHGA
                     VDPARVEHALALSEAQICPVWAMLKAGTPVSSSYAVVTG"
     gene            16646..17101
                     /locus_tag="VFZ86_05575"
     CDS             16646..17101
                     /locus_tag="VFZ86_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641795.1"
                     /translation="MRISTRLVVLTAAVVALVALTAASAGCGGDSPSASPSPEWEKVV
                     TSEVSGEAPVKLNLGTHQLGTQARLAWKLSGPDEPPVVLTFRLINADYGTGFGYSMTP
                     ETQGFQLDTENAMTVGPLKPGNFRIFFSQRFQDGAGPGYDGEVTVYTLK"
     gene            17207..19594
                     /locus_tag="VFZ86_05580"
     CDS             17207..19594
                     /locus_tag="VFZ86_05580"
                     /inference="COORDINATES: protein motif:HMM:NF012670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641796.1"
                     /translation="MSFTFGPRRGLYVDLTEEMPGGGPLTPVELDHFETFDLLYRSLC
                     ALLFNYVPTSGHPGGSISSGRLVAAALFDAMEYELGDPDRPDADLISYSAGHKAMGLY
                     AMWALRDEVAAVADPGLLPASPRQRLRLEDLLGFRRNPITGTPLFRAFGARALDGHPT
                     PATPFVKLSTGASGVGVASSIGLGFGAADRYGADAPRVHIIEGEGGLTPGRVSEALAA
                     AGTASLANVVLHLDWNQASIDSDHVCREGVLPGDYVQWDPRELFYLHDWNVIDVADGH
                     DVQQVVAAQRRALALDTGQPTAIVYRTHKGWRYGVEGKGSHGAGHKLCSEGFYEALAE
                     LSRTTGAAIDLPTCDPADRACAGPDGASVRERCFWTALQAIRAQVHAQLPMAAALAER
                     LAAARDRLDARERAPRPGAPRVDDAYELAEAAAQVAPAPEATPHQLRLEPGASTTLRG
                     EFGRALHHLQAASGGAFLVASADLMGSTSVGAIADGFPAGFWNARTNPDARLLSVGGI
                     CEDAIAGVLSGISAYGHDLGVGSSYGAFMAPLGHIAARLHAIGAQARQAASGEPYKPM
                     ILVCAHAGLKTGEDGPTHADPQPLQMLQEDFPKGTAISLTPWEPQEIWTLTAAALARR
                     PAVLSMFVTRPAEPVLDRPALGLAPPEAAVSGVYALRACEAPDVTVVLQESAVTYAFV
                     QEALPLLAEKGVEALVYYVASAELFDLLPEDERRAIFPEARAAQAVGITGFTLPTLYR
                     WVTSEAGRAASLHPFRQGHYLGSGPGETVLAEAGLDGASQAAAILAYLDARAAAR"
     gene            19614..20231
                     /locus_tag="VFZ86_05585"
     CDS             19614..20231
                     /locus_tag="VFZ86_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5641797.1"
                     /translation="MTEPREIAAAAGEVVPGLWTWSIANSAIGGHVSTSQAVLTREGC
                     LLIDPVRLDGAALGALPRPVATLLTARCHQRAAWRYRTELGAEVWLPEDASAADEEPD
                     HTYADGQLLPGGLRAFRTPGPEWPHYSFLSEDGPGVVFCSDLLSSDEAGLHFIDPAFH
                     EDPAATRESVEHLLELPFGILCLAHGAPLTGDPKAAIRTLLGRRA"
     gene            complement(20271..21524)
                     /locus_tag="VFZ86_05590"
     CDS             complement(20271..21524)
                     /locus_tag="VFZ86_05590"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX5641798.1"
                     /translation="MRSKSSPRRRTAAAAALALAVFALAAASPAVAFAQRFAVNGGAT
                     YARGLRATVGDAGWSPFFRPGVVVWDGGSIIAGYGADRGYRFPVQTLAVVPRTCASFV
                     SSTGGARIADMLADAPVEVDARYDAGADLNLCLVLAGGGDLRAGASVASVFDAVGTYC
                     RERRAAGFRVIVLTVLPSDRPETFDAARLAYNAMLRERWAEFADGLADIAADPRIGET
                     GDELDRQFYGVDARHPNNAGNAVMAAVTAPVVAAQPWTSARCELRLRDLPGDWSAWRP
                     YSAATTAQLGDYQGVHVVEAEYRLDGGEPVATADTIFVDTVRPVPRALRDVVVRRGRT
                     AVLRYRIDDADPCGPTGTAVVSVTTARGRVVKTFVRRRVPVGQRATIAFTCTLPKGSY
                     RYVVRARDAARNPELVPGTARLTVR"
     gene            21835..22626
                     /locus_tag="VFZ86_05595"
     CDS             21835..22626
                     /locus_tag="VFZ86_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641799.1"
                     /translation="MRLRKLTLAIALTALAAGAVAAPAVAGDPSISKLKQQLRQAKQL
                     RERARERARLAAAGLAGARELHAATSVAGDPAMLTPATPDPATLDPATPDPATPEPLS
                     ALVVPPVSMDPTLAAALLADGVVTADEVAAQQARSVRAGTLARRWTVKVRRLQQRIRT
                     LQQIAEWNRRGQWKPLIEIAGKKYGVGTAGLYRMMMLESGGSRTIGTTYKGLFQYYPS
                     TWSGSWNPWRHESIYNGWAQIRATAYALSRGMGRSQWPNTYPMAF"
     gene            22845..23441
                     /locus_tag="VFZ86_05600"
     CDS             22845..23441
                     /locus_tag="VFZ86_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641800.1"
                     /translation="MTVDATTPALARPRLDVGLSFKGGWNAFTADIVPLLVGGLIAGL
                     LSIVTLGILAGPLFAGLYSMVVGRIRDGRPARIGDVFSCMDRFWGFLWAAIVLVVLIG
                     LASLTIVGGVLLATIWLYVFPFMVDRRMGLAEAMTASYHQVVDNGFWEHLALVVIFIA
                     IGAVANGWLAILTTPFTIAVTAAAYFVSGGREDLVARR"
     gene            complement(23583..24044)
                     /locus_tag="VFZ86_05605"
     CDS             complement(23583..24044)
                     /locus_tag="VFZ86_05605"
                     /inference="COORDINATES: protein motif:HMM:NF016178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEX5641801.1"
                     /translation="MASVEQLWATFLEERPGVAGPGDTYSAWHFCDNQADADELVELV
                     LAGRKRATAGALWSYETEDEPLPRAGDFSVVTDWSGAARCIIRTVAVDVVAFGAVSDE
                     FAATEGEGDGSLASWREAHWAAFSRELEGTGRAPRPDMPVVCERFEVVFPV"
     gene            complement(24049..24639)
                     /locus_tag="VFZ86_05610"
     CDS             complement(24049..24639)
                     /locus_tag="VFZ86_05610"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011020752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5641802.1"
                     /translation="MFHDIPGAIRARMAQLEEIDARDRRDGTARLERLRQVPREVGRF
                     VALLAAGAPPGRWIEIGTSAGYSTLWLALACREVGARVTTFEVLDAKAALARETFALA
                     GVDDVVEFVHADVREHLAGLDGIAFCFLDSEKADYGELYEAVVPRLVPGGLLVADNAV
                     GFEDVMQPMLDGARADARVDAVLVRFLTGQLICRKR"
     gene            complement(24629..25276)
                     /locus_tag="VFZ86_05615"
     CDS             complement(24629..25276)
                     /locus_tag="VFZ86_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006944155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641803.1"
                     /translation="MTQERSLPRATLDALGGVPRFLRAPFHRRRHLRWGATDAEVADH
                     MPGDELVPRASFEATRAITIDAPPEAVWPWIVQMGYRRAGFYTYALLDNAGYDSADTV
                     LEEYQHPRVGDWMPMASKVSETTAFRVAAFATDEWLLWSKPDSTWSWTLTALAGGRTR
                     LVTRLKQRYPWESPGTAVFTLVLLELGDFPMMRRMLTGIKERAERLAKGGWTRVP"
     gene            complement(25359..25724)
                     /locus_tag="VFZ86_05620"
     CDS             complement(25359..25724)
                     /locus_tag="VFZ86_05620"
                     /inference="COORDINATES: protein motif:HMM:NF039496.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5655 domain-containing protein"
                     /protein_id="HEX5641804.1"
                     /translation="MTLDEFFEGFETSRPVFEAVREAVEATGLSTLRVSKSQIAFARR
                     RPFAWVWVPGRYLGPGRAPLVLSVALGHRDAWPRWKQVVEPSPGRFMHHLELREPGEV
                     DEELRSLLREAWECAGEGS"
     gene            complement(25759..26553)
                     /locus_tag="VFZ86_05625"
     CDS             complement(25759..26553)
                     /locus_tag="VFZ86_05625"
                     /inference="COORDINATES: protein motif:HMM:NF013213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX5641805.1"
                     /translation="MRFIDAHLHTDMIEDVQLQKLVMMGMEAAVIPSPHMFVGTHDAD
                     TVLLLWERFLTMEATTAQTLGYEAFTSISVPFFGVNRADAERCLERMPEFLAHERVVA
                     MGEIGLDCGTEFEEWLFREHLRLAREHDLPVILHTPIRLAPQGPAVTPRVFEIIREEG
                     YPVERCVFDHAAEETMDFRMSTGGMVGLSICWDKMPPEVAARLVIDHPEWRERLLINS
                     ELGGMGNDYFMVPRVQLAMKLFGLDKETIDQVCYRTPRDFFHLPVD"
     gene            complement(26571..27809)
                     /locus_tag="VFZ86_05630"
     CDS             complement(26571..27809)
                     /locus_tag="VFZ86_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5641806.1"
                     /translation="MSAPQHAPESIAGRGMSRGLILLFAVATGQAVACNYLAQPLLET
                     IRDLFGVSAAVAGLIVTAAQIGYAAGLILLLPLGDLFERRRLITTLAAITVVGLLAAA
                     AAPSIGFLIAAAGVIGTTSVMAQLLVPFAATLSPEAERGRVVGTVMSGLLLGILLART
                     FSGLVADLAGWRAVYLIAAVLMTAQAVLLYVKLPRYRQSAGLSYPKLLASVLDIARGE
                     PILRRRALYGALSFASFSILWTTLAFLLAGPAYGFSDGVIGLFGLVGAAGALTASVVG
                     RFTDRGWGYGLTGVSCASMLAGFGLLWLGGHHLSALLAGILVLDVGSTGVHISNQSEI
                     YRLQPAARNRINAFYMTTCFVGAAAGSATAALVYDRWSWTGACVLGLLVAVAATARWA
                     TDRQRPLAEKDPVCEAAPPA"
     gene            27932..28393
                     /locus_tag="VFZ86_05635"
     CDS             27932..28393
                     /locus_tag="VFZ86_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641807.1"
                     /translation="MADDPRTVVLALAEHEEAMSALYSSYAAQYPEVGDLWTSLARDE
                     HGHGALLRSLAEKSEDLTAFVANRRYDLADVQADTARLRNLAQITPVAGFPLQEAFRA
                     AVKLEDSLIESQIFTVHDGDPAAVTAVVDTLREQTEKHQRRLSEKLRGLGG"
     gene            28485..29753
                     /locus_tag="VFZ86_05640"
     CDS             28485..29753
                     /locus_tag="VFZ86_05640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HEX5641808.1"
                     /translation="MAASSSFVDFTPGRRAVIILIFGGLWTLGSFATDLFLPAMPAAA
                     EALGASESAVALSVTTFLIGLGLGQLIAGPVSDGRGRRPTLLVGLVVFTASALVCVVA
                     PTVEVLIAVRFAQGAAAAFGLAIPNAMVTDYARGREAARLFSWIVIIGGVAPLVASLT
                     GAQTLHLLGWRGPFVALTAISVFALVCVALWLPESLPRERRVTGGLRAALRTMAGLTR
                     DGRFMGYTFTGAFVYMAFFAYLGGSSFVFQHQYGVSATTYSILFAVNAVGMLAAGQAN
                     HRLLARFSPRSLLAVALIAFTVAGMAVLVSALTGAFGIWGLAVPFFVIVAGMGVILPD
                     LTALALSLHPEVAGTASAYFGGLRLGLGALAMPLIGISGTVTATSVAIMIAAAGTAAL
                     VLFGVTARHARDQQVLLDLPEEAASDVPIA"
     gene            29812..30081
                     /locus_tag="VFZ86_05645"
     CDS             29812..30081
                     /locus_tag="VFZ86_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641809.1"
                     /translation="MFCEIARKVEGDDLESIQSLEKELGLTLVAFSCRALDPAREERL
                     QKIMEELGPQLQAPPAEPGEAQLGRIRELEAELGLTLIAVEASPS"
     gene            complement(30097..30420)
                     /locus_tag="VFZ86_05650"
     CDS             complement(30097..30420)
                     /locus_tag="VFZ86_05650"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5641810.1"
                     /translation="MQTITAAQTAVADNPHGVDVRHLHKTPHVVVSQITLEPGEVVKP
                     HKAPVDAFFYVLEGTPAIEIEGETVLAGPDTLVPSALGHVHAIRNETQERVRFLVVKT
                     PNPKP"
     gene            complement(30558..31679)
                     /locus_tag="VFZ86_05655"
     CDS             complement(30558..31679)
                     /locus_tag="VFZ86_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641811.1"
                     /translation="MPDSLNGRVALQRVRARARLTARMAVSRARGRPHTLSHLVTAGC
                     NGSCATCLWRSGAGGRAAGANAAPAAGDRRAAGGELGTAAVRRLYAEAGRAGMAQLVV
                     WGGEPLLRQDLPELLLAARRSGLFVTLISNGWFAGERWPALRGAVDALILSLDDEGGA
                     HDRMRGLPGLFARLEGFAEELRSDPLRPTLLLNTVLSGQNRGALRRVAAVARRWGAGL
                     YFCPMETGELSAGEGGDRLAALALSQDELREAARLALSLKDAGYPVLDSRPYLRMLAA
                     DPALRSYTCRAPRARLTVGADGSVRDCRRVDRPLANVADLQAAGRPLADLFRAPVWRR
                     MGEEAAACTKCNNPDVIELSWLWDLRPAMLGKVAELAGR"
BASE COUNT         4424 a        11419 c        11481 g         4551 t
ORIGIN      
        1 gggaacccgg tgaggtcggt gtgcgggagg aagagcgccg acgtgtactc gtccatgaac
       61 gtgttgtcgg cgctcagctc caggtacgtc atcttgctcg ccacgtcggc gacctcgcgg
      121 cggacgttga cgcagagcag agtggcgaac gcccccaggg cgctcgtgtt gccgaggaag
      181 tggaagcgct ccacgggctg gtcgggcagg agcccgatgc ggatggcctt ctcgacgttg
      241 aggtactggc cgaacgcgcc gccgatgagc acctgctcga tgtcggcgag gtccacgccg
      301 acggaggcgc agagcacctc gaagccggcg tagatggccg ccttggcgcg gatgaggctg
      361 gtgatgtccg actcggtgag gacgatgtcc ttctcggcgc cgccctcgcc ggcccggcac
      421 accacgtact cgggcacccc atcgcgcacg cgcgcgcgcg ccgtctgcgc ctgctggtcg
      481 aggtgtccgg acttgtcgag cagcccggtc acgaagagct cgccgagcag gtcgatgagg
      541 ccgctgccgc agatgcccac ggccggcgcg tcgtcgatgg tgcggaacgt cggctcgagc
      601 gtcgcatcgt cgatgtagac gtcctcgatg gcgcccggcg acgcgcgcat gccgtcgcgc
      661 acgccgccgc cctcgaaggc ggggccggcg gagcaggcgc acgagagcag ccagtccttg
      721 gtgccgagga ccatctcccc gttggtgccg atgtcgatga acagggtcag cttgtcggtc
      781 gcgtacaggc ccgacgagat gacgccggcg gtgatgtcgc cgcccacgta ggcgcccacc
      841 gacggcagca cgtggacgcg cgcgaccggg ttggcgttga gcccgagctc gtgggcgacg
      901 agcttgggcg ccgagctgac ggtggggatg tacggttcct cgcgcaggta gcgcggctcc
      961 acaccgagca gcaggtgcgt catcgtcgtg ttgccggcga tggccacctc gtggatctcg
     1021 cgcatctcgg cgccgttccg ctgctggagc tcgtccaaca ggtcgttgat cgtctcgatc
     1081 accagcttct ggagccgctc cagcccgtgc ttccgcttgg cgtagatgat gcggctgatg
     1141 acgtcctcgc cgcaggagat ctgcttgttg tacgcgctcg ccgtgtcgac gacgcggccc
     1201 gtggtgaagt cgacgaggta cgcgacgagc gacgtcgtgc ccaggtcgac ggcgagcccc
     1261 atgctcgcct gggggaacgc cgtcgggtag acgcgcagca ggcgcggcgg caggtacgag
     1321 ccgtacaccc agtcgcgcat ctcgagcgcc acggtcacct tccagttggc gtggcgcagg
     1381 gcggtgccga gccgcctgag ctgcgggagc tcggcgcgga cctcggtgat gccgtgctgc
     1441 tgctggaggg cgcgacgcag ccggtcgaag tcgctggtct gatcggccag cgacggcggc
     1501 tcgatgacga gctcgaagag cctgaccgcc gggttctgga ggtagtcgca ggtgatgggc
     1561 agcgactcgg gctcggcgac tgcgtgcccg tgcgggcgga ccttctcctt gcgccgctcg
     1621 ggcacctcca cggagaccga gcccgagacg taggtctggc aggccaccgc ccagccgtcg
     1681 aggatctcgg cggtggcgag gccggcgttg ctgcgccgct cgacctcgcc gctcgtgatg
     1741 cgcacgcggc agcggccgca ccgtccctgc cccgcgcacg gggcagggac gtccagctcg
     1801 cagaggatcg ccgcgtcgag caggctcgta ccctgcggca cctcgatcgt ctccccggac
     1861 ggcgtgaagg tgatcgtcgc catcgccgtc cgcggcctcg tggcaggagt ctcgctcatg
     1921 cgccccagac gttcttgatg tagttgggga tgtccacggc gtcacgcggt ccgaccatga
     1981 tcttccagtc gggcagttcc tcttcgacct cgcccgagag cccggcgacg tggccgggga
     2041 tgaccagctt cttgtggacg accttctgct cgatgccgga gatcttgacc gtcttggcga
     2101 tcttctcggc gtcgaacttg ccggccgccc agccggtcag cacagactgg ccgtcgctgt
     2161 cggcgacgag cagccacgtt gggatgcccg cgccctcgac ctcgcccgcc accgagaagt
     2221 aggtgagcga gaagttggtc gtgatgagca ccgggctgtc ggcggtgacg gcgccgatct
     2281 cgtagagctt gggctccatc tggatcggtt tctgcgggtc ggtgtagatg ttctggcgca
     2341 gcgtgagcag cgtgtagagc atcgccggct cgaagtggtc gacgatgatg acgccggcgt
     2401 acttgccgac ggcctgggtg gcacgtacca gctctgcctg ccacgtcggc gccgccgcga
     2461 cggcgacgag cgggtagccg agctcccgcg cgcccttctt gagcgccagc cggcggagct
     2521 gcgtggcggc ggcgaggtcg ccggcgaggg tcgtggcgcc ggggtcaagg accaggtcct
     2581 ccacgccggc gccgcggacc tgcccggaga gctcgaggag gccgtccagg tcgccgtccg
     2641 tgaccttgat gaccagcggg cagccgtgct tcttggctgc ctcggccatc gcctgccagt
     2701 tcgccgcgat ggcggcgtgg atgagcggct tctcagcggc ggcgccgccg gccaaggccg
     2761 cgtccaccgc cgccgcatcg acgctcatga gcaccagcgg caggccggga gcggccgcac
     2821 gtgctgcggc cacggcggcg gcgtacgcgt ccgcgccgcc gcccgcgtcc tcgacggcga
     2881 ggccgtcgag cgtgagcgtc atgcctacgc gctcgacact gtacgcggcc acgcgcgccg
     2941 ccacgtcgcc gaggtcggcg gcgtcgctct tgacgcgcac cacgaggccg ggctggtgga
     3001 cgaacgtctt ctcgtgccgg tacatcaccg tctcgttgcc gaccgcgaag gcgcggtcgc
     3061 cggcgccgat cgtcgcgagc ttgatcggcg gcgccgacgc ggcgtccagc gccgtcttgg
     3121 cttcgtcact cacgtacggg cacttgtcga gctcggtgcc cttctgcgcc agcttcatgg
     3181 cgaaggccag gcaggtcggg aacccgcact ccttgcagtt ggtctggggc agcagcttgt
     3241 agatctgcag tccgctgagg gccatcgctc aggctccctt cgtcaggtcg tcgagggcct
     3301 cgcgcacggc gctcacgctg gccgggtgtc gcaggaccag gatgtcggcg ccggactcga
     3361 gcagcgtcgt cgccgtgctg atctcccagg cgacgccgcg ctcggcaagc gcgccccacg
     3421 cctcgggcac gtcgccctcg gcgcggcctt ccttctggcg ccacgcctct tcgccggcgg
     3481 tgcagatcat cggctgctgc gtcatggagt cgcccatgag ggccgccagg cgcaggcgct
     3541 ccatcaccga gtacgtgtac tcgaggccgt agccgagcgc gcccgtgttg gggtccatga
     3601 gcacgcgctc gagcgggagg cccatgtcac tgatcaggat gttgagctgc ttggcgaggt
     3661 tgacgtcgat cggcgtctgg gcgatcacca ggtgcccgtt gccaagcgcc gcagcgacga
     3721 tggtgcggta gttcttgtcc tcgcaaaggc cgagggcgag ctgctcgccc ttggtcttct
     3781 cggcgacggc gacgagcacc tcgttgtcct tgtcggccga gccgggcccg tacacgatga
     3841 gcggcaccgg cacggcggcc agcacgcgct cgacggtgcg cacggcgtcc tcgcacgagc
     3901 cgtctccctg gtcggggtga cagctgcgga gcttcagggc gatcaggtcc gccccggcct
     3961 ccacggcctt ctgcgcccac gtggcggcgt cgtccatggc gtcgccccac gccgcctgca
     4021 gctcggcgga ccagtccgcc gggtgcatgt cctgcacctc gacggcgatc gccggctggt
     4081 gcggcatggc gccctcgaag ccgaggaacg gcaaggcggt ctctccgccc acggtcacgg
     4141 tgctgccacg cgtcccaccg ccgtagctgc tgccgagcgt gacctcgcgc acggcgccgg
     4201 accacttctc tactgggatc tcgactgggg tcatctgcac cctccgggtg tcaggaaaag
     4261 gggactcaga acagcgggtc catggtcagg gccgggtgcc ccttctcctc gaggaaggga
     4321 aggagatcct cgaccgtggt ggccacggtc tcgtcggcga tcttgtcgag caggtcgggc
     4381 acgccctccc gctcgcagac ctcctgcagc gcggggcgca tgctctcgcg caggttggag
     4441 ctcagccaga cgatacgctt catgccgccg tcggccgaga tgaacttcct gctcgtgagg
     4501 tagtacttgc ccatgcccat catgccgggc gtctgcatgc cgccgccggc ggttccggcg
     4561 agggtgctga aggtcatgcc gcagggcgtc atgctcgggt cctcgcggct gacgatcatg
     4621 aagccgttgg cctcgggcac gagcaccatg atggcctcga agcagccgca cgaggtcagg
     4681 ggagcctcca tgatggagta gagggtcacc ctctccaccg actggttgga gttctggtag
     4741 atgtactcgt tggggccgct gaactcgccc ttctcggcgt cgatgacgag acccttcgcg
     4801 accggctggt tcgggcccgt ggggttgatc tcgtagctcg ccttgcagtc gagccagttg
     4861 taggcgccgc acagacccac gcgctccggg ttgatgacgc agacgtggtt gggcgcgaag
     4921 ctctggcaca gcaggcagct gtagaaggtg tcgaccgact cgtcggtgag gttggcgatg
     4981 cgctcgttgc gctcggcgta ggcgacgcgc gcgacctcga tgagctcgtc gacctcggcc
     5041 tggtcggtgg tgaggtgcac ctgcaccttg tcgacgatgt tgccgaacac ctcgtggatg
     5101 cgcgcgtgga tgaccttgcc gaagtgctcc aggcggaagc ccttgccgaa ggtgctcttg
     5161 ctcacgcgcg tccaggcgat gtcgcgctgg ccgttgtgct ggatgccctc gacgccgttg
     5221 atgaagtagt ggaactggcg ctcgaggatg ggctcgaagt cggtctgcat cttgcggccg
     5281 gagacgtcga cgaccatacc gagcggcagg gcgccgccct cctccatgcc gtcgatgtcg
     5341 gtgccgctga cggtgatctt gccgtcctcg acctcgtcgg tcggcttcat gcgcaggaac
     5401 tcgaacgcgt tcgaccgctt gccgccgaac tcggcgtaca tgatgtcctt gcggatgcgc
     5461 tcgccctcga aggccgatcc gtaactgacg gggatcggca ccttggcgat cttgaccttg
     5521 acgccgcgca cctccatgca gcgctggatg agcttctccg tgcgctccat gtcgtcggcg
     5581 ccggggatct cgtcgaacgg catgctcacc acgtgctcgt aggtggtcac gccggtcggg
     5641 aggatctgcg ggatgcgcgt gtcggcgatg gtcgggaagc cgaagctgat ggcgccggcc
     5701 gcggtcgcgt acttgaggtc gtcgacctcg ccgagcgcga gcacgaaggc gaacacgcgg
     5761 tacttgttgt agagcatgat cttgcgcgcg ttgcccgcgt ccatgccgcc gaacgtgagc
     5821 gcggagcggg cggcgaaccc gagcgcgtac acggcggaga tggtgtcgat gccgaacggc
     5881 accgtgaagg tgtcgtagcc gagttggatg ccctcttcct gcaactggtg gatgatgctt
     5941 cgcccgttga cgttgccgct gaggaagatg aggatgccgc ggctctgcag ctcgcggacg
     6001 atcttgaccg cgacctcgtt gctcctggcg cagccgacga tggcgcagaa cccgggcatg
     6061 cggccgtcga ccagctggat gccccaggcc cgcagctgga tgtcgtcgat cgggccgttg
     6121 agcttcatct tgtcgccgtg cacgccgagg tcgccgccgg tgaagcgggc cttgtcctcg
     6181 cgcgcctgtt cgagcttgat cacctgcggc tcgctgccgc gggcgaagcg cacgccctcg
     6241 atcgcctcct cggcgaacag ggtggcgatg ccgctgtcga gcgtctcgcc gaggtacggc
     6301 agccacatct ggtcgccggg gatcgggtgc agcaggctct tggcccgctc gatcaccggc
     6361 ggcaggtcgc ccagcttctc caccttctgg ccggtgaacg cgtagatcac cggcaggtag
     6421 tacgcggtgt tggtgaactg gaccggcgtc tccgggccca gctcggcgag cgccgcgttc
     6481 acggcctctt ccgcctcgcg gacgacgccc tgggcgccgc ggatggcccg ggtcgcgatg
     6541 tagcgagaca tcaggcctcc ccctcgtcgt gcgtgtgcgt gtggccgtcg ccgtggtcgt
     6601 gggtgtggcc ctcggcgagc gggtagacgg gcgagccgac ggtctcgacg ccgcgctcgg
     6661 cctccgcagc cttgtcggcc aggtacctct tgatctcggc gaagaagagg ctgtcgccgc
     6721 tgtcgccgta ctggctcggg tcgtactggg ggagcttgag cgccgcgcgc ttggcgtcga
     6781 tcagctcgag cgagcgcgcg tacatcgcgt cccagtccgg gatgaactcg aggtccccgc
     6841 cgaccagctc ctcccagccc tcggtcatga ggcgcgtggc gacgctggag gcctccaccg
     6901 ggctgctcac gccgaagatg acgtgggcgc cggaggcggc gaagtaggtg ccgatggcga
     6961 tggccttctc gcacatccac tcggggcaga ggccgacggc cggcaggtcg tcgatgtcct
     7021 cccccaggcc gccctcggtg gcgacctgcg tcaggacggt gaggatgcgc gagttgtcga
     7081 cgcaggagcc gaggtggagc accggcggga tgccgatcgc ctccatcacc tcgcgcaggc
     7141 cggggccggc gtggtcgagg atctccgggc tcaggtaccc ggccttggct ccggccgtgg
     7201 cggcgcaacc ggtcacgacc accatcacgt cgtcggcgat gaagcgcttg atcatctcga
     7261 cgatgccctc gtcctgggtg acgcgcacgt tgttgcagcc gacgacgccg gcgagtccgc
     7321 ggatgcggcc ggcgatgatc gcgtcgttga gcggccggaa cgagccgcga tggaagccgc
     7381 cctgcatgta gttgaggtac tcgtggctga atccggcgac cagcggctcg tggaagtccg
     7441 ggatgtggac cgggccgcgg ttcgggtagt tggcgatcgc catgcgcacg atctcgcggg
     7501 ccgtctccgg ggcgcggtgc tcgtcgaact cgacgtgcgt cgcgccgggg atcttggcct
     7561 tcggcgaggt cgtcacgagc tgcgtgtgga agcgctgggc gagcggcccg agcgcctgca
     7621 tgatgcactg cacgtcgacg accatggtct ccaccgcgcc ggtgagcacg gccagctcct
     7681 ggtgcaggaa gttgccggcg gcggggatgc cgtagcgcat gaggatctcg ttgctggtgc
     7741 agcagatgcc ggcgaggttg atgccctccg cgccggccgc cctggcctcg gcgacgagct
     7801 ccgggtcgcg agcggcctcg accagcatcg ccgacagcgt cggctcgtgg ccgtggacga
     7861 tgacgttgac ctggtcgtcc ttgagcacgc cgaggttgac ctcgctcgac agcggcgagg
     7921 gcgtgccgaa caggatgtcg ctgatgtccg tgctcagcat cgcgccaccc cagccgtcgg
     7981 ccagggagca gcgcagcgcc gacttgagga tgttctcggc gtcgaggtcg acgccctcgt
     8041 gcgtgcggtg cagcacctcg accacctcgc ggtcgatggc gcgcggggtg agtcccagct
     8101 cgcgccacct ggcctggcgc ctcggggtcg cgcgcctggt gtagacgatc tcgccgtgcg
     8161 gctgaccgaa ctgcgcgagc gcgacgtcgg ccacgtcgag ggcgatctcg ttgacctcgc
     8221 ggccgctcgt cgggatgccg aagtaccccg ccacggagtg cagcttctgc tcgtccttga
     8281 tcttgtagcc cgcggcctcg cccatggcca cggcgcgcag cgtcatggcc atgtcgcggc
     8341 cgtggtcgga gtgggcgctg acgccgcccg cgacctgccg ggcgaagtgg cgggcggcga
     8401 tcgtcggcag gtcggcgccg cagatcccgg tcttctgctc ggcgtccttg ccgacgaggc
     8461 ggcagggccc catcgagcag atgcggcagc atgcgccgga ctcgccgatg gggcagggct
     8521 tcatggtgtc cgcgcgagag aacgcggtct cgagcccgca gccctcggca tgcgccagca
     8581 tctcgagggc ggccggatcg atgctcttcg cggctaccgg cttgacgtcg tcgttgatct
     8641 tctcagccat gaggcgactc cttcacgccc gggcaggaac gggccgcgac gcggctgcgg
     8701 cccggaggtc cgggcggatc gtggacgaga gaacgcgcac aggtcggtcg gcgatcagta
     8761 cttgcggtgg tcgcccgaca tcgtgaggtc ggccggatcg gtggtggtga cgcgcacccc
     8821 cgcggtcagc cacgccggct tgtccgtcgg cggggcatcc gcctcggggg tctcgccggc
     8881 ggcatagccg gcctgttcga gctgtgcgac gatgcgctgc gggtcggtct tcgccgggtc
     8941 gtactccaga cgaagcgtcg cgtccatcgc gctcgcgccg gtgacttgga cgtcctccgc
     9001 ttgctggaag agacctcgga cggcgatcac gtggtggtcg gcccacatgc ccgggacgct
     9061 cagggccagc tgctccattc gtgcctcctc gcgcgaacgg tccttcgggg aggcgaccgc
     9121 cgggggaggc gggacgtcac cccttgacaa gaacctaggg gggtatccgc aactcagtgt
     9181 gcgcgactgt acgacatggc gcaaccgttg gtaaaggatt gtgcttattg tcacaaaatg
     9241 tcgaacatcg gcgcacgtaa acaccaccga aatgcgcgaa aacgggccgc cgggcgaaca
     9301 gaaacgctca cccggcggcc cgatcgcggt ccattccgtg acgcgagatt ctcctcaggc
     9361 cgggacgtcg agccgctcgc ggcgcacgcc gcgcagggcg ctgcccgcga gcgcggcgac
     9421 ggccgcgccg gccacgaacg acaccggctg catccagctg acgtagccga tcatcgcgac
     9481 ctggacgacg atccagctca tcagcgtcac acccatggcc agcgaaagcc agggggcgac
     9541 acggctcttg aggatccagg cggccgtggc gacggccatg ccgccgccga tcaccgtgaa
     9601 gaggaagagg ctcgggacga agtagctgct gaacggcgtg ccgtcgagcc aggccgggtc
     9661 cacgccgggc gcgccggaga gaccgtagta gccgcctccg aaggcgttca gcatcacgag
     9721 caactggatg gtgatgatgg cgatcctcgt gcgcttcatg gttccgcctc ccgggcattg
     9781 cgccccgtca ggttctgctg tacggtaggt aacagcgtta ctgtaacgtc gttaccgtaa
     9841 cagtgtttac aagcgctgtc aacggattcc ttcgggatct gccggactca gggagcggac
     9901 atgggcggac gaggcggagg caggcgcgag aggaccgggc cggtgacggc ggaggtcgtc
     9961 gtcgacgccg ccctcgcggt catcgacgcg gaggggctcg acgccctcac catgcgccgg
    10021 ctggcgcacg acctgggcgt cgagcccgtc accatctacc ggcagctgcc gaacaaggac
    10081 gccatcctcg ccggcgtcgc cgagaagctg tggcgagaga tggccccccc tgatgcgggc
    10141 gaccaggggg aggccccaag ggcgccagcg ggcgcggatg cgccggactg gcgcgcgcag
    10201 gtgcgcggca tgtggctggc gctgaacgag ctcatgcagc gccaccccaa cgcgatcccg
    10261 ctcatcgccc gcggaggctc ctactcgccg agcgccgggg agggcaccgc cggcatgctc
    10321 cggatcttca aagacgcggg cctgacgccc gaggaggcgg cggagctgct gcacatcctc
    10381 tccgcctgcg tggtgggctt cgggttcgct tcgctctggg tccggcagat cgccgccgcc
    10441 cggaaggccg cgccggcggg cgacgagctc gagccgacgc ggccgccgga cgaagccggc
    10501 gacctcgcgg actacctcga ggcgacgcgg cgctgggatc cggcgcagtt cacggccgcc
    10561 gtcgacatcg tgctccgagc gtacggcgac gacgccaagg atcgagctgc cgcagcaccg
    10621 caccgcggca ggccgactac tgctgcttga cggcgacgag cgcgacgccc agcctgtcct
    10681 cgagctgctt gaggcggtct atctgcgcgg cgtcgatcgg agcggcgtcc atctggtgac
    10741 aggcgaaggc gaggatcggg acgcccagct ccccctccag cgacgtgatc tcctcgatct
    10801 gcccctggtc gagcttggtg gcgaggctgc acagcatggc acggctcctt gtgcgcgggt
    10861 gggcggtcac ccatgccttc ccccactccc ccgggacgac aaccgttgac cggagccggg
    10921 gcgcagcccg tacgcgcggg cggcgcggcg ggcggccggc aatgagccac gacgtcgcct
    10981 catcgagggg tgcaccggag gcgcacccct catagggcca cccgccgcgc tgcggcggtc
    11041 gtgacgtcag gccgtgaccg gcgcgggcgc cggccagcag aggccgtact cgtcgatctc
    11101 cgcgtaggtg acgccgccga ggctcgggat gagcagcgag atctcgccct cccacacggc
    11161 cttggggtag gcggcctgcc actcgtggcc gagccgcctg gccacctcgc ggaaggtcca
    11221 ccaacccggc cgcgccgcgc ctgcagggcg ctggacacgc cgcagacggc tgatgcgccg
    11281 ctcggcgttg gtgaacgtgc cgtcctcctc gccccaggcg acggccggca ggaccacgtc
    11341 ggcgacggcg cacaaatcgc cgcgcatggc cccctggacg acgacgaagt cggccttgcc
    11401 gagcgcggcc agcagggcct cggcgtcgcc gaacgacgcc gcctcgttgg cccacaccca
    11461 ggctgccttc agctcgcccc cggccagggc ctcgacgatg cccgccgtag agcggccggg
    11521 ggcgtccggg atggcttcga ccccccacgc atcggcgagc ctctgccggg cctccgcgtc
    11581 ggtgacgagg tcgccgccgg gcagcacgtc gggggcaacg cccatggcca gaaggccctg
    11641 gacgttgccg gcgccgagcg gcagcgagac gctgcgcgcg ccggcgaggt cgtcgagcag
    11701 ggactgcagc ttgaccacgg ccgcgccggt gccggcgagg tcgtcggtgg tgcccagggt
    11761 cgcgcagacg acggccggcc ggacgtcgac cagcttggcg gcgatctccc ggatggtgac
    11821 ggcatccaca ccggcgtcgt ccgcgatgcg ctcggggagc acggcggcga gcatcgcctc
    11881 gagctcgcgc tcgtgcggca gcgcctcgcc ggcgtcccgg cgcccggcaa gctcgttggc
    11941 cagcagcacc gcgatgatgc cgttgaggat ctcggggatg gccgccggct ccaccgcgag
    12001 acgcaccgcg gcggcgtcgc cgaggtcgct gtgaccggag tcgagcacca tgaggtgggc
    12061 gccgtcgcgg acggcgcgac gcagggcggt gccggccacg gggtggtcgg ccacggcgcc
    12121 gtcgccgacc accaggacgg cgcgcgcctc ctcgagcacg gtcagggcgc cggaggtgta
    12181 gcacacgccg gcgccggtcc cggtggccgc gagcgagacg cgcgacgctt cgatcgcccg
    12241 gtggtcgatg tcgttggtgc cgagcacggc gcggaagaac ttctgggcct ggtaggcctc
    12301 ctcgttggtg cacaccggcg cgatgaggcc ggcgactccg ccggcgccga caccgccgag
    12361 cctctcggcg accaggtcga gcgcctcgtc ccagcccgcc ttgacgagca cgccgttgcg
    12421 gcggacgagc gggtcctcca ggcgcgcgcg ctcgtccggc tccagcacgg cgaagcggcc
    12481 gcgcttgcac agccggcccc ggttcggcgg cacgtcgccg gcgccggtca ctttgacgac
    12541 ctcgccgtcc ttgaggtgga ccaccgtctg gcagcccatg ccgcagtgcg ggcaggtggt
    12601 gcggatgcgc tgcagctccc agtgccgggc cacgcccttc gcggtgcgct ccgtcagcgc
    12661 ccccacaggg caggcgtcga cgcactggcc gcagagctcg cactggtcaa ggtcgggcag
    12721 ggggtaccag tccttgccgc cggcgcgctc ggcgcgccgg ccggacgggt ggggcaccac
    12781 gccgtgcacc tggatggccg tgcaggcggc ccagcagcgg ccgcagagga tgcacttgga
    12841 gtagtcgcgg atgacgtagg cgtcctcgac gagcgggaat tcctcggcgc ccggcagctc
    12901 gctcggcggc tccacctggt actcgtaggc gagccggctg agctcgcact cgccgagcgc
    12961 gtcgcaggtg atgcaggtgt ggcgtcccga gttgatgagc agctcgagcg tcgcgcggcg
    13021 cgagtgcctc acccggtcgc tcatggtccg gacgtccatg ccctcggtgg ccggcgtgct
    13081 gcaggcaggc agcagccggc cggcgccggc cacctccacc acgcagaggc ggcagacgtc
    13141 gcggtgaccc gcctcgggca ggtagcacag ggtggggatg gcgatgccgc cgccgttcgc
    13201 cacgtcgagg atggtctggc cgggctcgaa ggcgagcacc cggccgtcga tggtgatctg
    13261 gggtgcgccg gtcggcggtg cgtccgtcat ctcagaccac cgccacggcg atgcggtagc
    13321 agcgcagaca gcgctcggcc tcgtgccggg cgtcggcggc gctcaccgct ccttcgactt
    13381 cgtcgaaaca ctggatgcgc acctccgggg gcacgaccat ggggcgcagc ttctcggtgc
    13441 tgccggtgaa cggtatcggt gcctgcgcct cgtcgcggat gacggcgagc atcgcgtgct
    13501 cgagccgctc ctccagcgcc ggggcgacgt cgcagccccg cagcaacgcg tcgatggaca
    13561 cggcggcgcg cctgccggcg gcgagggcgg cgacgaggct gctgggcccc gtgtagcagt
    13621 caccgccgcc gaacacgcgc gggtcggcgg actgcatggt gagcgggtcg acctcgagcg
    13681 tcttccactt gctcacctcg atgtggctcg gcatgagctg gtcgacggag cagacctggc
    13741 cgattgccgg gacgatgcag tcgcacggga tgacgaactc cgagccctcg acgggctcgg
    13801 ggcggcggcg cccggaggag tcgggctctc cgagctgcat gcgccggcac tcgaggccga
    13861 cgaccttgcc gtcctcggcc atgatcttcg tcggcgcgac cagggtgacc atctcgacac
    13921 cctcctcctt ggcctcgacg atctccaccc ggtcggccgg catctcggcc tcggtgcggc
    13981 ggtagagcag cttgacgtcg tcgaagccga tgcggcgggc ggaacggacg cagtccatgg
    14041 ccacgttgcc cccaccgatc acgagcatcg tcttgccggt gagcggcgtc ttgccgaagg
    14101 cgatctcgcg caggaactcc accccgggca tgaagccgtc gtagccggcg tcctcgccct
    14161 cgcagcccat gcgggccgac tggtgcgcgc cgacggcgat gaacacggcc tcgtagccct
    14221 gggcgaagag gtcgtccatg gtggcgtcga cgccgaagcg gaagttgtac tcgatcttga
    14281 cgccgagtga ctcgacgcgc tcgacctcgg cgcgcagcac gtcgcgcggc aggcggtagt
    14341 cggggatgcc gaccgcggcc atgccgccgg cctccggcag cgcctcgaag atcgtactga
    14401 tgatgccgtc cagccccagg tagtaggcgc aggcgaggcc ggccggcccc gcgccgatga
    14461 tggcgacgcg cggcttgccg ggcatggggc aggcgatggg ctcgggctcg ctgtcggcga
    14521 cgaagcgctt gagcgtgcgg atggcgaccg gctcgtccac cgagccgcgg cggcacgcgt
    14581 cctcgcaggg acggacgcag acacggccga tcgtcccggg catggggcag cgccggcgca
    14641 cggcgtcggc cgagcgctcg acctcgtcga tgcgcagctg ctccacgtag gccgggatgt
    14701 cgacgccggc ggggcaggcg gccatgcacg gcgcgacgac cgtggaggcg tacacgccgc
    14761 gcgggatgga ctccttcgcg gcgaccgcgg cggcgaagcg gtcgtagtgc tgctcgaggg
    14821 actcgaggac ggcgtagccg ccggtgcggc cgaggtcgca gcgcgacgcc tcggcgacgt
    14881 gccggctgag ctcgagcgcg cgctcgagcc gggcctcggt gccctcgccg gaacagatct
    14941 cctcgaggat gtcggcgacc tcgtgcatgc cgatgcgaca ggggaagcac tggccgcagc
    15001 actcctggac ggtgcgggcg gcgaactccc gggtgaggtc cacgatgttg acggactcgt
    15061 cccacacggc cacgcccccc cagcccatga cggcgcggaa ggtgccggcc agctcgtcgg
    15121 ggagtcgcga agggtcggtg gtccaggcac ccgccacggc gacggcggcg tcggtcatgc
    15181 agcctcctct gcgccggtcg ggcactggct cggcggctgc ctccctccag cggcagtgtg
    15241 gagggcccgg accggacggt tcgtcctcgg cgccccagcg gagcgtctct caacgcacaa
    15301 tgatacgttc gagcgccgcg agccacaatc gagctcggac gttggcggga acgcgcgtgg
    15361 agcgagcgga ggccgggacg tgccggcggc ggcgtcaggc gcccgagagg gcgcggcgag
    15421 cggcgtcggc gagcgcgacc ggcgactggg cgccgctcac cgcgaagcgc ccgccgaaca
    15481 cgaacgtcgg gacggcgcgg atccccagct cggcggcgcg gcgctcgtcc agccgcaccg
    15541 ccgtcgccgt gaggtcgccg tcgagggcgg ccgccacccg ctccgcgggc atcccggccg
    15601 cctccgcaag gtcctcgagc accgcgcggt cgccgacgtc cgcaccgcgg gcccagtagg
    15661 ccgagagaag gtcgaggccg aggcgctgct gcagctcctg ccccacgtcc tctccctcct
    15721 ccagcgcccc ggcgagaagc cggtgagcga ggaacgtgct cgggcgccgc tcgagctcgc
    15781 ggagatcgac gagcggcagg ccgtccgccg cggcggcgtc tcccagccgt gcggccattc
    15841 cctcgatcac ccctgggccg tacttgccct cgaggtaggc ggcgaagccc gtcccctcgg
    15901 gagggacggt ggggtcaagc tcgaacgccg cgaaccgcca ggtcacccct tcggcggcgt
    15961 cgccgagccg tctgagcgcg gtgagccagc gcgacgcgcc gacatagcac cacggtcaga
    16021 cgatgtcgga ccagatgacg acctcgacca cgggtcctct cctcttcgca ggccggtcgc
    16081 agggtggttt ccccatctgc ggccgggcca gtccggccgt gccgccgacg ggcccggcga
    16141 cggacgcgac acgcggcctc ccggcctgag agaatgtccc cggccacgca tcaccagcaa
    16201 ggagcgccca tgccgtccga actcagcgtc cacgccgtgc agcacggccc catgcggttc
    16261 gccatcagtg acggcgacca cgaggtgacc gtggactacc cgctgcccgg cggcgacggc
    16321 gacctcgggg ggatgacccc gctgcgcctc ctccttgcca gcctcgcggg ctgcagcggc
    16381 agcgctgtgg ccgcgcttct ccggcgcgac gggcagcccg tggagcgtgt cgaggtgacg
    16441 gcgcgcgggc ggcggcgcga cgagcacccc accgtgatca ccgacatcga cctggcgttc
    16501 gtggtccacg gcgcggtcga ccccgcgcgc gtcgagcacg cgctggcgct gtccgaggcg
    16561 cagatctgcc cggtgtgggc gatgctcaag gcgggtacgc ccgtgtccag cagctacgcc
    16621 gtcgtcaccg gctgaggagg caccgatgcg gatcagcacc aggctcgtcg tcctcaccgc
    16681 cgccgtggtc gcgctcgtgg ccctgacggc cgcgagcgcc gggtgcggcg gcgactcccc
    16741 gagcgccagc ccgtctcctg aatgggagaa ggtcgtgacg agcgaggtct cgggcgaagc
    16801 gccggtgaaa ctcaacctcg gcacccacca gctgggcacg caggccaggc tcgcctggaa
    16861 gctctccggt cccgatgagc cgccggtggt cctcaccttc cggctcatca acgccgacta
    16921 cggcaccggc ttcggctact cgatgacgcc cgagacccag gggttccagc tggacacgga
    16981 gaacgcgatg accgtggggc cgctcaagcc ggggaacttc aggatcttct tcagccagcg
    17041 gttccaggac ggcgccgggc cggggtacga cggagaggtc acggtctaca cgctcaagta
    17101 gccgcggcgc cgcggacgca cggggtggtg cgccggtcgc cgggagcgga gtagaatcct
    17161 ggtatagtcc aggggcgcgg acgcgccggg gaaaggagcg gggaccatgt cgttcacctt
    17221 cgggccgagg cgtggcctgt acgtcgacct cacggaagag atgcccggcg gcgggccgct
    17281 cacgccggtc gagctggacc acttcgagac cttcgacctc ctctatcgct ccctctgcgc
    17341 cctcctcttc aactacgtcc ccacgtcggg ccacccgggc gggtcgatct cctcgggccg
    17401 cctggtcgcc gcggcgctct tcgacgcgat ggagtacgag ctcggcgacc cggaccggcc
    17461 ggacgccgac ctcatcagct actcggccgg ccacaaggcg atgggtctct acgccatgtg
    17521 ggcgctgcgc gacgaggtcg cggcggtggc cgacccgggc ctgctgcccg caagcccgag
    17581 gcagcgcctc cgcctcgagg acctcctcgg cttccgccgc aacccgatca ccgggacgcc
    17641 gctgttccgt gcgttcggcg cccgggcgct cgacgggcac ccgacgccgg cgaccccctt
    17701 cgtcaagctc tccacgggcg cctcgggcgt cggcgtcgcc agctccatcg gcctgggctt
    17761 cggcgccgcc gaccggtacg gcgccgacgc cccccgcgtg cacatcatcg agggcgaggg
    17821 aggcctgacg cccggacgcg tgagcgaagc gctcgccgca gccggcaccg cctcgctcgc
    17881 caacgtcgtg ctgcacctcg actggaacca ggcgtcgatc gactcggacc acgtctgccg
    17941 ggagggcgtt ctccccggcg actacgtgca gtgggacccg cgagagctct tctacctgca
    18001 cgactggaac gtgatcgacg tggccgacgg ccacgacgtg cagcaggtgg tcgcggcgca
    18061 gcgccgcgcg cttgccctgg acaccgggca gccgacggcg atcgtctacc gcacgcacaa
    18121 gggctggcgc tacggcgtgg agggcaaggg cagccacggc gccgggcaca agctctgctc
    18181 cgagggcttc tacgaagcgc tcgcggagct gagccgcacg accggcgccg cgatcgacct
    18241 gcccacctgc gacccggccg atcgggcgtg cgccggcccg gacggcgcga gcgtgcgcga
    18301 gcgctgcttc tggacggcgc tccaggcgat ccgcgcgcag gtgcatgcac agctgccgat
    18361 ggcggccgcg ctggcggagc ggctggcggc cgcccgggac aggctcgacg cgcgcgagcg
    18421 ggccccccgg cccggcgccc cgcgcgtcga cgacgcctac gagctggccg aggcggcggc
    18481 gcaggtcgcc ccggcgcccg aggcgacgcc gcaccagctg cgcctcgagc ccggcgcgtc
    18541 cacgacgctg cgcggcgagt tcggccgcgc cctgcaccac ctgcaggcgg ccagcggggg
    18601 cgccttcctc gtggcctcgg ccgacctcat gggctccacc agcgtgggcg ccatcgccga
    18661 cgggttcccc gccgggttct ggaacgcgcg caccaacccg gacgcgcgcc tgctcagcgt
    18721 gggcggcatc tgcgaagacg ccatcgcggg cgtgctcagc ggcatcagcg cctacggcca
    18781 cgacctcggc gtcgggtcct cctacggcgc cttcatggcg ccgctcggcc acatcgccgc
    18841 gcgtctccac gccatcggcg cccaggcgcg ccaggcggcg agcggcgagc cgtacaaacc
    18901 gatgatcctg gtgtgcgcgc acgccggcct caagaccggt gaggacggcc ccacacatgc
    18961 ggacccgcag cccctccaga tgctgcagga ggacttcccc aaggggacgg cgatctccct
    19021 gacgccgtgg gagccgcagg agatctggac gctgaccgcg gcggcgctgg cgcggcggcc
    19081 cgccgtcctc tcgatgttcg tgacccggcc ggcggagccg gtgctggacc ggccggcgct
    19141 cgggctggcg ccgcccgagg ccgccgtctc gggcgtgtac gcgctgcgcg cctgcgaggc
    19201 ccccgacgtg accgtcgtac tccaggagag cgccgtgacc tacgccttcg tacaggaggc
    19261 gctgccgctg ctggcggaga agggcgtgga ggcgctggtc tactacgtcg ccagcgccga
    19321 gctcttcgac ctgctgccgg aggacgagcg gcgcgcgatc ttccccgagg cgcgcgccgc
    19381 ccaggccgtc ggcatcaccg gcttcaccct gcccacgctc tatcgctggg tgacgagcga
    19441 ggccggccgc gccgccagcc tccacccgtt ccgccagggc cactacctgg gcagcggccc
    19501 gggcgagacg gtgctggccg aggccggact ggacggagcg agccaggcgg cggcgatcct
    19561 cgcgtatctc gacgcccgcg ccgccgcccg ctgaccgtgc acgaggagga acgatgaccg
    19621 aaccgagaga gatcgcggcc gcggccgggg aggtcgtccc cggcctctgg acgtggagca
    19681 tcgccaacag cgccatcggg gggcacgtct cgacgtccca ggcggtgctg acccgcgagg
    19741 gctgcctgct catcgacccg gtgcgcctcg acggcgcggc gctgggcgcg ctcccgcgcc
    19801 ccgtggcgac gctgctcacc gcccgctgcc accagcgcgc cgcctggcgc taccgcacgg
    19861 agctgggtgc ggaggtgtgg ctgccggagg acgcctcggc cgccgacgag gagccggacc
    19921 acacctatgc ggacggccag ctgctgcccg gcgggctgcg cgccttccgc acgcccggcc
    19981 ccgagtggcc gcactactcg ttcctctccg aggacgggcc cggcgtcgtc ttctgttcgg
    20041 accttctgag cagcgacgag gccgggctgc acttcatcga cccggccttc cacgaggatc
    20101 cggctgcgac gcgggagagc gtggagcacc tgctcgagct cccgttcggc atcctgtgcc
    20161 tggcgcacgg agcgccgctc accggcgatc ccaaggccgc gatccgcacg ctgctcggcc
    20221 ggcgggcctg acccgcggag ggtgcgcggc gcgcccgctc ggggcggtgc tcagcgcacc
    20281 gtcaggcgtg cggtcccggg gacgagctcg gggttgcggg cggcgtcgcg cgcgcggacg
    20341 acgtagcggt aggagccctt gggcagggtg cacgtgaacg cgatcgtcgc ccgctgtccc
    20401 accgggacgc ggcggcggac gaaggtcttg accacacgtc cgcgggccgt ggtgacgctc
    20461 acgacggccg tgccggtggg gccgcaggga tccgcgtcgt cgatgcggta gcgcagcacc
    20521 gccgtcctgc ccctgcgcac cacgacgtcg cgcagcgccc gcggcaccgg gcgcacggtg
    20581 tcgacgaaga tggtgtccgc ggtggcgacg ggctcaccgc cgtcgagccg gtactccgcc
    20641 tccaccacgt gcacgccctg gtagtcgccg agctgcgccg tggtcgccgc cgagtaagga
    20701 cgccaggcgc tccagtcgcc gggcaggtcc cgcagccgca gctcgcagcg ggccgacgtc
    20761 cacggctgcg ccgcgacgac gggagcggtc accgcggcca tcaccgcgtt gccggcgttg
    20821 ttcgggtgcc gcgcgtccac cccgtagaac tgccggtcca gctcgtcgcc ggtctcgccg
    20881 atgcgcgggt cggcggcgat gtcggcgagg ccgtcggcga actccgccca gcgctccctg
    20941 agcatggcgt tgtaggcgag ccgggcggcg tcgaaggtct cgggacgatc cgagggcagg
    21001 acggtcagga cgatgacccg aaagccggcc gcccggcgct cgcggcagta cgtcccgacc
    21061 gcgtcgaaca ccgacgccac gctcgcgccg gcgcggaggt cgccaccgcc ggccagcacc
    21121 aggcagaggt tcaggtcggc cccggcgtcg tagcgggcgt ccacctccac gggagcgtcc
    21181 gcgagcatgt cggcgatgcg ggcgccaccg gtggaggaca cgaagctcgc gcaggtcctg
    21241 ggcacgacgg cgagtgtctg gaccgggaac cggtagccgc ggtctgctcc gtagccggcg
    21301 atgatgctgc cgccgtccca gaccaccacg ccggggcgga agaagggcga ccagccggcg
    21361 tcgccgaccg tcgcgcgcag gccgcgcgcg tacgtggcgc cgccgttcac cgcaaagcgc
    21421 tgcgcgaacg ccacggccgg cgatgccgcc gccagcgcga acaccgcgag cgccagagcc
    21481 gcggccgcgg cggtgcgccg ccgcggcgaa gacttactcc tcatgcctac ccccatggtc
    21541 ccgtgagcac ctctgcggct cacgggcgcc agtctatacg gccgcccttg cggggccgag
    21601 caggcggcgc gcgaggctcg gctggggcct ccgggttgca ccgcgcgcga taatccgctt
    21661 gactgtgcgg cggatctcac caagaggcgc ctcgcgccct ccgcccgcag attcgtcttc
    21721 ctccccattc accgttgacg gacatgtcgt gcggcggcgt gcgtgcggcg cggaccgccc
    21781 gtccggcaca ggccatcacc gctgccgcgc gggacatcac gtggaaagga caacgtgcgt
    21841 ctcagaaaac tcaccctcgc catcgccctc accgctctcg ccgccggcgc cgtcgccgcg
    21901 ccggcagtgg caggcgaccc ctcgatctcc aagctcaagc agcagctccg ccaggcgaag
    21961 cagctcaggg agcgcgcccg cgagcgcgcc cgcctcgccg ccgccggcct ggccggcgcc
    22021 cgtgagctcc acgccgcgac cagcgtcgca ggcgacccgg ccatgctcac cccggccact
    22081 cccgatccgg ccacgctcga tccggccact ccagatccgg cgactcctga gccgttgtcc
    22141 gcgctcgtcg tcccgcccgt ctccatggac ccgaccctcg ccgcggctct gctggccgac
    22201 ggggtggtca cggccgacga ggtggcggca cagcaggcgc ggagcgtccg ggccgggacg
    22261 ctggcgcgcc gctggacggt caaggtgcgg cggctccagc agcggatccg cacgctgcag
    22321 cagatcgctg agtggaaccg gcgcggccag tggaagcccc tcatcgagat cgccggcaag
    22381 aagtacggcg tcggcacggc cggcctctac cgcatgatga tgctggagtc cggtggcagc
    22441 cgcacgatcg gcaccaccta caagggcctg ttccagtact accccagcac ctggtcgggg
    22501 tcctggaacc cgtggaggca cgagagcatc tacaacgggt gggcgcagat ccgcgccacc
    22561 gcctatgcgc tgagcagagg catgggccgc agccagtggc ccaacaccta tccgatggcg
    22621 ttctaggaga gtcccgagac tcgcgccggc ggccggaccg gaccacccct ccggccgccg
    22681 gcctctccct ccggcgccgg cggcatcccc ctgcgggggc accccgccgg cgcccaagtt
    22741 cctcctccag ttgaccccgg gttcagccca ccgggagacg accgcggccg gccctcgccg
    22801 taggctctcc ccaggcaacg cactcaaggg gaaaggaact caccatgacc gtcgacgcca
    22861 ccacaccagc gctcgcgaga ccccgcctgg acgtcgggct ctccttcaag ggcggctgga
    22921 acgccttcac cgcagacatc gtcccgctcc tcgtgggcgg cctgatcgcc ggcctcctct
    22981 cgatcgtcac gctggggatc ctcgccggcc ccctgttcgc cgggctctac agcatggtcg
    23041 tcggcaggat ccgcgacggc cgccccgccc ggatcggcga cgtgttctcc tgcatggacc
    23101 gcttctgggg cttcctctgg gcggcgatcg tgctggtcgt gctgatcggg ctcgcctctc
    23161 tgaccatcgt cggcggcgtg ctgctcgcga ccatctggct gtacgtgttc ccgttcatgg
    23221 tcgaccgcag gatgggcctg gccgaagcga tgaccgcgag ctaccaccag gtcgtggaca
    23281 acggcttctg ggagcacctc gccctggtcg tgatcttcat cgccatcggc gcggtggcca
    23341 acggctggct cgcgatcctc acgacgccct tcacgatcgc cgtcaccgcc gccgcctact
    23401 tcgtctccgg cggccgcgag gacctggtcg cgcggcggta ggccggcccg gcggaagccg
    23461 gacggcgcca ggccggacgg gcccggcgga gggttctccc ctccgccggg cccgtcgtcg
    23521 tctcccctcg cggccgccgg tcctctcggc ttctcgcggg cggcctcccg ccgcaccgcg
    23581 tgtcagaccg ggaagacgac ctcgaagcgc tcgcacacca ccggcatgtc cggccgcggc
    23641 gcgcgcccgg tcccctcgag ctcccgggag aacgccgccc agtgggcctc acgccaggag
    23701 gccagggagc cgtcgccctc accctcggtc gcggcgaact cgtcactgac ggcgccgaac
    23761 gccacgacgt ccacggcgac ggtgcggatg atgcagcgcg ccgcgccgct ccaatcggtg
    23821 accacgctga agtcgccggc ccgcgggagc ggctcgtcct cggtctcgta ggaccacagg
    23881 gcgccggccg tggcccgctt gcgcccggcg aggacgagct cgacgagctc gtcggcgtcg
    23941 gcctggttgt cgcagaagtg ccaggccgag tacgtgtccc ccggtccggc gacgcccgga
    24001 cgctcttcga ggaacgtggc ccacagctgt tcgacggagg ccacgcgcct accgcttcct
    24061 gcagatcagc tggccggtga ggaagcgcac gaggacggcg tccacgcgcg cgtcggcgcg
    24121 cgcgccgtcc agcatcggct gcatcacgtc ctcgaacccg acggcgttgt cggccacaag
    24181 caggccgccg ggcacgagcc tgggcacgac ggcctcgtag agctcgccgt aatccgcctt
    24241 ctcggagtcg aggaagcaga aggcgatgcc gtcgagcccc gcgaggtgct cgcggacgtc
    24301 ggcgtggacg aactcgacga cgtcgtccac gccggccagc gcgaaggtct cgcgcgcgag
    24361 cgcggccttc gcgtcaagga cctcgaaggt cgtcacgcgc gcgcccacct cgcggcaggc
    24421 gagcgccagc cacagcgtcg agtagccggc gctcgtgccg atctcgatcc agcgcccggg
    24481 cggcgcgccc gccgcgagga gggccacgaa cctgccgacc tcgcgcggca cctgccgcag
    24541 gcgctcgagc cgcgcggtcc cgtccctgcg gtcgcgcgcg tcgatctcct cgagctgcgc
    24601 catgcgcgcc cgaatggccc cggggatgtc atggaacacg ggtccagccc cccttcgcca
    24661 ggcgctctgc gcgctccttg atcccggtga gcatcctgcg catcatcggg aagtcgccca
    24721 gctcaagcag gaccagggtg aagacggccg tgccgggcga ctcccacggg tagcgctgct
    24781 tgaggcgcgt gaccagccgc gtccgacctc ccgcgagcgc agtcagggtc caggaccacg
    24841 tgctgtccgg cttggaccac agcagccact cgtccgtggc gaaggccgcg acacggaacg
    24901 ccgtcgtctc actcaccttg ctcgccatgg gcatccagtc gccgactcgg gggtgctggt
    24961 actcctccag gaccgtgtcc gcactgtcgt agccggcgtt gtccaggagc gcgtaggtgt
    25021 agaacccggc gcgccggtag cccatctgga cgatccacgg ccacaccgcc tccggcggcg
    25081 cgtcgatggt gatcgcgcgc gtcgcctcga acgaagccct gggcacgagc tcgtcgcccg
    25141 gcatgtggtc ggccacctcg gcgtcggtcg cgccccatcg caggtgccgc cgccggtgga
    25201 acggcgcgcg gaggaaccgc ggcacgccgc cgagcgcgtc cagcgtcgcg cgggggagag
    25261 atcgctcttg cgtcatgggc gcctcccgtc cggcgagacg cgcgcctcca gctcctcgcg
    25321 catgccgcgc agctggtcga gcgacggcgg cgtgtccatc aggacccctc ccccgcacac
    25381 tcccacgcct cgcgcagcag ggaacgcagc tcttcgtcca cctcgcccgg ctcccgcagt
    25441 tcgaggtggt gcatgaagcg gcccggcgac ggctcgacga cctgcttcca gcgcggccac
    25501 gcgtccctgt ggccgagcgc gacggacagc acgagcggcg cgcggccggg accgagatag
    25561 cggccgggca cccagaccca ggcgaacggc cgccgccgcg cgaaggcgat ctgactcttc
    25621 gacacgcgca gggtcgacag gccggttgct tcgaccgcct ctcggaccgc ctcgaagacc
    25681 ggccgcgacg tctcgaaccc ctcgaagaac tcgtccagcg tcatatagcg cactccccga
    25741 ccgcgccgta tccgccaatc aatccaccgg caggtggaag aagtcgcgcg gcgtccggta
    25801 gcagacctgg tcgatcgtct ccttgtcgag gccgaagagc ttcatcgcca gctggacgcg
    25861 cggcaccatg aagtagtcgt tccccatccc gccgagctcg gagttgatga ggaggcgctc
    25921 gcgccactcg gggtggtcga tgacgagcct ggccgcgacc tcgggcggca tcttgtccca
    25981 gcagatggag aggccgacca tgccgccggt gctcatgcgg aagtccatgg tctcctccgc
    26041 ggcgtggtcg aacacgcagc gctccaccgg gtagccctct tccctgatga tctcgaacac
    26101 gcgcggggtg acggcgggcc cttgcggcgc cagacggatc ggcgtgtgca ggatcaccgg
    26161 caggtcgtgc tccctggcca gccgcaggtg ctcgcggaac agccactcct cgaactccgt
    26221 gccgcagtcg aggccgatct cacccatggc cacgacgcgc tcgtgggcca ggaactcggg
    26281 catgcgctcg aggcacctct cggcgtcggc gcggttcacg ccgaagaacg gcaccgagat
    26341 ggaggtgaag gcctcgtagc cgagggtctg cgccgtggtc gcctccatcg tgaggaaccg
    26401 ctcccacaga agcagcaccg tgtcggcgtc gtgcgtgccg acgaacatgt gcgggctcgg
    26461 gatgaccgca gcctccatgc ccatcatcac gagcttctgc agttgcacgt cctcgatcat
    26521 gtcggtgtgc aggtgggcgt cgatgaatct catgccggtt cctttcgtgg tcatgcgggc
    26581 ggcgccgcct cgcagacggg gtccttttcg gcgagcggac gctgccggtc ggtcgcccag
    26641 cgcgcggtgg cggccaccgc gacgagcagg ccgagcacgc atgcgcccgt ccagctccag
    26701 cggtcgtaga caagggcggc cgtggcggag cctgcggcgg ccccgacgaa gcaggtcgtc
    26761 atgtagaagg cgttgatgcg gttgcgcgcc gccggctgca gccggtagat ctcgctctga
    26821 ttgctgatgt ggacgccggt cgagcccacg tcgaggacga ggatgccggc gagcagcgcc
    26881 gagaggtggt ggccgccgag ccagaggagg ccgaagcccg cgagcatcga cgcgcaggac
    26941 acgccggtga gcccgtaccc ccagccgcgg tcggtgaagc ggcccacgac cgacgcggtg
    27001 agcgcgccgg ccgcgccgac caggccgaag aggccgatga cgccgtcact gaagccgtag
    27061 gcggggccgg cgagcaggaa cgccagcgtg gtccacagga tgctgaacga ggcgaacgag
    27121 agcgcgccgt acagcgcccg gcggcggagg atcggctcgc cgcgcgcgat gtccagcacg
    27181 gaggcgagaa gcttcggata ggagaggccg gccgactgcc ggtagcgtgg cagcttgacg
    27241 tagaggagca ccgcctgcgc ggtcatgagc acggcggcga tgagatagac ggcacgccag
    27301 cccgcgaggt cggcgacgag gccggagaac gtgcgcgcca gcaggatgcc gaggaggagg
    27361 ccgctcatga ccgtcccgac gacgcggccg cgctcggcct cgggcgagag cgtcgcggcg
    27421 aagggcacca gcagctgcgc catcaccgac gtcgtgccga tgacgccggc ggccgcgatc
    27481 aggaagccga tcgacggcgc ggccgccgcc gccaggagcc ccacgacggt gatcgccgcc
    27541 agcgtcgtga tgagccggcg ccgctcgaag aggtcgccga gcggcagcag caggatcagg
    27601 cctgccgcgt agccgatctg ggcggccgtg acgatgaggc cggcgaccgc cgccgagacg
    27661 ccgaacaggt cgcggatggt ctccagcagc ggctgcgcca ggtagttgca ggcgaccgcc
    27721 tggccggtcg cgaccgcgaa cagcaggatg aggccgcggc tcatgccgcg gccggcgatg
    27781 gactcggggg cgtgttgcgg agcgctcaca cgccctgcct acaagccgcg gcggaagctg
    27841 tcaaggccgc ggtccattcc acggaacgga cacgttgtcc gtatggtgga agtgacatag
    27901 catcgtggca cattccctgg aggtgtgacg catggcggat gacccgcgca cagtagtact
    27961 ggcgctggcg gaacacgagg aggcgatgag cgccctctac tcctcctacg ccgcgcagta
    28021 ccccgaggtc ggcgacctct ggacgtcact cgcccgtgac gagcatggtc acggcgccct
    28081 gctgcgctcc ctggcggaga agagcgaaga cctgacagcg ttcgtcgcga accggcggta
    28141 cgacctcgcc gacgtgcagg ccgacacggc acgactgcgc aacctcgcgc agatcacacc
    28201 cgtcgcgggc tttccgctgc aggaggcgtt ccgtgcggcc gtcaagctgg aggattcgct
    28261 gatcgagtcc cagatcttca ccgtccacga cggtgacccg gccgcagtga ccgccgtcgt
    28321 cgacacgctg cgcgagcaga ccgagaagca ccagcggcgg ctgagcgaga agctcagggg
    28381 gctcggcggc tgacgccggg aactgcgcac gacgaccgcg ggcgctcgcg ggcgcgcgag
    28441 ctgcgcccgg gcgcgcccgc cggcatgacc gtctgttgag ccgcatggcc gcgtcctcgt
    28501 ccttcgtcga cttcacgccc ggccgccgcg ccgtcatcat cctcatcttc ggcggcctct
    28561 ggaccctggg gtcgttcgcg accgacctct tcctgcccgc gatgcccgcg gcggccgagg
    28621 ccctcggcgc gtccgaatcc gccgtggctc tcagcgtcac gaccttcctc atcgggctcg
    28681 ggctcggtca gctcatcgcc ggccccgtga gcgacggccg cggccggcgg ccgaccctgc
    28741 tcgtcggact ggtcgtgttc accgcctcgg cgctcgtctg cgtcgtcgcg ccgacggtgg
    28801 aagtgctgat cgccgtgcgt ttcgcacagg gggcggcggc cgccttcggc cttgcgatcc
    28861 ccaacgcgat ggtcaccgac tacgcgcgcg ggcgtgaggc ggcccggctc ttctcctgga
    28921 tcgtgatcat cggaggcgtg gcgccgctgg tggcatcact gaccggcgcc cagacgctcc
    28981 acctgctggg gtggcggggg ccgttcgtcg cgctgaccgc gatcagcgtc ttcgccctcg
    29041 tgtgcgtcgc gctctggttg ccggagagcc tgccgaggga gaggcgggtg accggcggcc
    29101 tgcgcgcggc gctgcgcacg atggccgggc tgacacgtga cggccgcttc atgggctaca
    29161 cgttcaccgg cgccttcgtc tacatggcct tcttcgccta tctgggcggc tcgtcgttcg
    29221 tcttccagca tcagtacggc gtctcggcga cgacctacag catcctgttc gccgtcaacg
    29281 ccgtaggaat gctggcggcg ggccaggcga accaccgtct cctggcccgc ttctctccgc
    29341 ggagcctgct cgccgtcgct ctcatcgcct tcaccgtcgc cggcatggcg gtcctggtct
    29401 cggcgctgac cggtgcgttc ggcatctggg gcctggcggt gccgttcttc gtgatcgtcg
    29461 ccggcatggg cgtcatcctg cccgacctga ccgcgctggc gctttcgctc caccccgagg
    29521 tggccgggac cgcgtccgcc tacttcggcg gtctccggct cggcctcggc gccctggcga
    29581 tgccgctgat cggcatcagc ggcacggtca cggcgacatc cgtggcgatc atgatcgccg
    29641 ccgcggggac ggccgcgctc gtcctcttcg gggtcaccgc gcgccacgcc cgcgaccagc
    29701 aggtcctgct cgatctgccg gaagaggcgg cgtcggacgt gcccatcgcc tgaggcggcg
    29761 gcgcccgcga ggtacactct cgccaacggc ggacgtcaca aggagcgcac catgttctgt
    29821 gagatcgcga ggaaggtcga gggcgacgac ctcgaaagca tccagtcgct ggagaaggag
    29881 ctcggcctga cgctggtggc gttctcgtgc cgcgcgctcg acccggcacg cgaggagcgg
    29941 ctgcagaaga tcatggagga gctcggcccg cagcttcagg cgccgccggc cgagccgggc
    30001 gaggcgcagc tcgggcgcat ccgcgagctc gaggccgagc tcggcctcac gctgatcgcg
    30061 gtggaggcgt cgccgagctg acggtcaccg cccccgtcag ggcttggggt tcggcgtctt
    30121 gacgacgagg aagcgcacgc gctcctgggt ctcgttgcgg atcgcgtgca cgtgcccgag
    30181 cgcgctcggt accagcgtgt ccggcccggc caacaccgtc tcgccctcga tctcgatcgc
    30241 cggcgtgccc tcgagcacgt agaagaaggc gtcgaccggc gccttgtggg gcttcacgac
    30301 ttcgccgggt tccagcgtga tctggctgac gacgacgtgg ggcgtcttgt gcaggtggcg
    30361 cacgtcgacc ccgtgggggt tgtcggcgac ggcggtctgg gcggcggtga tggtctgcat
    30421 ggggactccg ttctcctgtg cgctggcccg tgcgggcgga tcgtcatcct gagcttgccc
    30481 acccgcggcc ggcgacgacc ttgaccctca tcaagctcgc cggcggacgg gacccgcgaa
    30541 cgggtcgcac cacagggtca gcgccccgcc agctcggcga ccttgcccag catcgcgggg
    30601 cgcaggtccc agagccagga cagctcgatc acgtccgggt tgttgcactt ggtgcaggcg
    30661 gcggcctcct ctcccatacg ccgccagacg ggcgcgcgga acaggtccgc cagcggccgg
    30721 cccgccgcct gcaggtcggc gacgttcgcg agcggccggt cgacgcggcg gcagtcgcgg
    30781 acggagccgt ccgcaccgac ggtcagacgt gcccgcggag cgcggcaggt gtaggagcgg
    30841 agcgccgggt ccgcggcgag cattcgcaga tacggccgcg agtcgagcac cggatacccc
    30901 gcgtccttga gggatagggc cagccgcgcc gcctcccgaa gctcgtcctg cgacagggcg
    30961 agagcggcga gccggtcgcc gccctcgccg gcgctgagct cgccggtctc catcgggcag
    31021 aagtagagcc cggcgcccca gcgccgggcc accgcggcca cgcggcgcaa cgcgccgcgg
    31081 ttctgcccgg acaggacggt gttcagcagc agcgtcgggc gcagggggtc gctgcgcagc
    31141 tcctccgcga agccctcgag ccgtgcgaag agcccgggca gcccgcgcat ccggtcgtga
    31201 gctcccccct cgtcgtcgag gctgaggatc agcgcgtcca ccgcgccgcg cagcgccggc
    31261 cagcgctccc cggcgaacca gccgttgctg atgagcgtga cgaagagccc ggagcgtcgg
    31321 gcggcgagga gaagctccgg cagatcctgc cggagcaggg gctcgccgcc ccacaccacc
    31381 agctgcgcca tcccggcgcg cccggcctcc gcgtacagcc ggcgcacggc cgcggtaccg
    31441 agctcgcctc cggcggcccg cctgtctcca gcggcgggcg cggcgttcgc gccggccgcc
    31501 cggccgccgg cgccggaccg ccagagacac gtggcgcagc tgccgttgca gccggccgtg
    31561 acgaggtggc tgagcgtgtg cggacgcccg cgggcgcggg agacagccat cctggccgtg
    31621 agccgcgccc gggctctcac cctctggagg gcgacacggc cgttcagtga gtcaggcatg
    31681 catgagtggt accacatccg cgggcgcggc atgccgccgg cccccgcgcg tttgcacccg
    31741 gtgagccggg gaacgccttc cgtgtgacac cgactcatcg aaaggaggct gacgccccat
    31801 gtcagctgaa ctgaccggca agcgcatcgc gatcctcgcc accgacggcg tggagggcgt
    31861 cgagctcacc agccc
//
LOCUS       DASUMX010000114         5441 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1755902, whole genome shotgun sequence.
ACCESSION   DASUMX010000114 DASUMX010000000
VERSION     DASUMX010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 5441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5441
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1755902"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..1196)
                     /locus_tag="VFZ86_05660"
     CDS             complement(144..1196)
                     /locus_tag="VFZ86_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017750899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HEX5641812.1"
                     /translation="MNGKRRIVTLSVLLGTLLLSLTLTTSAFASPVSSKKAQLREVQA
                     KLQGVYHQVDVAVEKYNQATTQLTTVQAQIKENERLLKVAEYNLDIANQQLSARAEDI
                     YKARDVGVVDVLFAANSFDDLVTQLNLMERLGNSDVDTVKSIAAYRQDIRDRRVKLGA
                     DKKAAVKLVASAAENKAEVESLQGKLEQMTAGIKADLRRLEQQAAARAQAAAQAAAAQ
                     QTGSTAGGGGGTVVDPGGSGHSSVVGIAQRYLGVPYVYGGASPSGFDCSGLTMYCYAQ
                     IGIGLSHGATDQQRASTQVPLSALQPGDLVFFGSASYSHHVGIYVGGGSMIHAPHTGA
                     VVSYGSISGAWIGGRP"
     gene            complement(1355..2767)
                     /locus_tag="VFZ86_05665"
     CDS             complement(1355..2767)
                     /locus_tag="VFZ86_05665"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX5641813.1"
                     /translation="MKIRLIEPEPPGMHVYAKVLLPRLGLPIIAATLKQHGHDVLVYN
                     PTFAPIDWDDVGSSDLVGLSSTTSTATTAYGFADELRAKDIPVVIGGSHVTFMADEAL
                     EHVDYVARGEGGEQLMLELIEALAGHRELESIAGLSFRRDGLAVHNQPRAALADLDEL
                     PFPDLRLLVGSDKLTTMPIMTSWGCPFDCTFCSVTAMFGKKYRFRSAESVIAEIKDKR
                     PQRIFFYDDNMAANRKRLKKLLRMMIDEGLVIPWSAQVRTDVVRDPELLDLMRRSGCQ
                     LVYLGLESVNQETLDKFEKAQTVADIERAIKLLHEYGIKSHGMFVLGADSDDVQTVRD
                     TVAFAIKNKIDTVMLNILTPLPGTQQFEEFDAAGRIFDKRWELYDAHHAVFEPARMTP
                     YELQIEVLRGYMRFYSLRTWLRYIFTMQFTKQLLFHWWGMVIIRNWRKDERNKAFIKA
                     LKRMWPRPTPRGTVRQARVD"
     gene            complement(2986..3978)
                     /locus_tag="VFZ86_05670"
     CDS             complement(2986..3978)
                     /locus_tag="VFZ86_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004099195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HEX5641814.1"
                     /translation="MVLLIAIVILALVFDYTNGFHDTANAIATSVSTRALSPRTAVLM
                     AASLNLLGALVSTSVAKTVGEGIVETDLVTLQVVMAALVGAVVWNFATWHFGIPSSSS
                     HALFGGLIGAMIASAGVDGVIWSGVVEKIILPMIGSPLLGFIGAWVLMTGLIWLVRKR
                     QLAPVNKWFRRLQPISAGFMAFSHGANDAQKTMGIIALALFASGQIDTFTIPTWVKIV
                     CALAMALGTYSGGWRIIHTLGTKVIKLDPIHGFAAETAAASVIQVATHFGVPVSTTHT
                     ITAAIMGVGSTQRLSAVRWGVAGNIVTAWILTLPAAGLVGAGVYLAGEAIRAIF"
     gene            complement(3993..4607)
                     /locus_tag="VFZ86_05675"
     CDS             complement(3993..4607)
                     /locus_tag="VFZ86_05675"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HEX5641815.1"
                     /translation="MKLSLVPKEHDYFRLFSELAANLDAAAQLLPRFMSDGDRKSIAA
                     AILEHEHVGDKIVHDIIRRLNKSFITPIDREDIYDLVATTDEILDNIEAATDMVMLYR
                     VGEITEQAKRQAEVIARATPLLKECMDNLEKPKGLDERIIAINSLENDGDRILRDAMA
                     SLFDGEMKCTDIIKWKDIYEILEAAIDECEHVANVIESIVLKHN"
     gene            4859..5374
                     /locus_tag="VFZ86_05680"
     CDS             4859..5374
                     /locus_tag="VFZ86_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641816.1"
                     /translation="MKKVLLIVVVAALALTLVPLAIAGGHGGGGHKNGKYKFNLVGNV
                     VAPDSDPAVEPAVDPAADPDAAPVVADPTEPAEPAVVSTIFIKVKAGSHIKGLRGTEK
                     MFDVAPGVKVWQLTADGAVEGRLADIEPDDRVKVKGWISKDGSKVYTITSIKYRDLTP
                     VVEETAPPAAR"
BASE COUNT          827 a         1725 c         1962 g          927 t
ORIGIN      
        1 cgtgcgccgc ggggcccttc ggcccggcgc ccgctcacta gctcttccgt gcattagtcc
       61 ggggtcgggg tgcagacgta ttcgatcctg cgggcgcgtt ccctacgcac gccgggagag
      121 cgcgccccca ggctcgcgga agatcagggc cggccgccga tccaggcgcc gctgatggag
      181 ccgtagctca ccacggcgcc ggtgtgcggg gcgtggatca tgctgccgcc gcccacgtag
      241 atgcccacgt ggtggctgta gctggcgctg ccgaagaaca cgaggtcacc cggctggagg
      301 gcgctgaggg gcacctgggt gctggcgcgc tgctggtcgg tcgcgccgtg cgagaggccg
      361 atgccgatct gcgcgtagca gtacatggtg aggccgctgc agtcgaagcc gctcgggctg
      421 gcgccgccgt acacgtaggg cacgccgagg taccgctggg cgatgccgac gacggacgag
      481 tgccccgagc ctccggggtc gacgacggtg ccgccgcctc ctcccgcggt gctgccggtc
      541 tgctgcgcgg cggcggcctg cgcggcggcc tgcgcgcgcg cggctgcctg ctgctccaga
      601 cgtctgaggt cggctttgat gccggcggtc atctgctcga gcttgccctg gaggctctcg
      661 acctcggcct tgttctccgc cgccgacgcg acgagcttga cggccgcctt cttgtcggcg
      721 ccgagcttga cccgccggtc tctgatgtcc tggcggtagg ccgcgatgct cttgacggtg
      781 tcgacgtcgc tgttgcccag ccgctccatg aggttgagct gggtcacgag gtcgtcgaag
      841 ctgttcgcgg cgaagaggac gtcaacgacg ccgacgtcac gcgccttgta gatgtcctcg
      901 gcccgcgccg agagctgctg gttggcgatg tccaggttgt actcggcgac cttgagcaac
      961 cgctcgttct ctttgatctg cgcctggacg gtggtgagct gcgtggtggc ctggttgtac
     1021 ttctccacgg ccacgtcgac ctggtggtag acgccctgca gcttggcctg cacctcgcgc
     1081 agctgtgctt tcttgctgct gacgggagag gcgaacgcgg aggtggtgag ggtgagggag
     1141 aggaggaggg tgccgagaag gacagagagc gtgacgatcc gtcgtttgcc gttcactgca
     1201 tcgcctcctt ttgggcgccg cggctgtcgc ggaccaccct cgttgaggtg agccctcacg
     1261 tgctctgcac tcaccgaact caagtacgag accctagcat ccgggccgcc cggtgacaac
     1321 cgcggcggcc cgttcgctga caccgctgcg tgcgtcaatc gacgcgcgcc tgacgcaccg
     1381 tgccgcgcgg ggtggggcgc ggccacatgc gcttgagggc cttgatgaac gccttgttgc
     1441 gctcgtcctt gcgccagttg cggatgatca ccatgcccca ccagtggaag agcagctgct
     1501 tggtgaactg catggtgaag atgtagcgca gccaggtgcg cagcgagtag aagcgcatgt
     1561 agccacgaag cacctcgatc tgcagctcgt agggcgtcat gcgcgccggc tcgaagacgg
     1621 cgtggtgcgc gtcgtagagc tcccagcgct tgtcgaagat gcggccggcg gcgtcgaact
     1681 cctcgaactg ctgcgtgccg gggagtggcg tgaggatgtt gagcatcacc gtgtcgatct
     1741 tgttcttgat ggcgaaggcg acggtgtcgc gcacggtctg gacgtcgtcg ctgtcggcgc
     1801 ccagcacgaa catgccgtga ctcttgatgc cgtactcgtg cagcagcttg atggcgcgct
     1861 cgatgtccgc cacggtctgc gccttctcga acttgtccag cgtctcctga ttgaccgact
     1921 cgaggccgag gtagacgagc tggcagccgg agcgccgcat gaggtcgagc agctccgggt
     1981 cgcggacgac gtcggtgcgg acctgggccg accacgggat gacgagcccc tcgtcgatca
     2041 tcatccgcag cagcttcttg agccgcttgc ggttggcggc catgttgtcg tcgtagaaga
     2101 agatgcgctg cggccgcttg tccttgatct cggcgatcac gctctcagcg ctgcggaacc
     2161 ggtacttctt tccgaacatc gccgtcaccg agcagaaggt gcagtcgaac gggcagcccc
     2221 agctggtcat gatcggcatc gtcgtgagct tgtcgctgcc cacgagcagg cgcaggtccg
     2281 ggaacggcag ctcgtcgagg tcggccaggg ccgcgcgcgg ctggttatgc accgcgaggc
     2341 cgtcgcgcct gaacgaaagg ccggcgatgc tctcgagctc gcggtggccg gccagcgcct
     2401 cgatgagctc gagcatgagc tgctcgccgc cctcgccgcg ggccacgtag tcgacgtgct
     2461 cgagggcctc gtcggccatg aacgtgacgt gggagccgcc gatgacgacg gggatgtcct
     2521 tggcccggag ctcgtcggcg aagccgtagg ccgtggtcgc ggtggaggtc gtgctggaga
     2581 ggccgacgag gtcggaactg ccgacgtcgt cccagtcgat gggcgcgaag gtcgggttgt
     2641 acacgagcac gtcgtggccg tgctgcttga gcgtggcggc gatgatcggc agtcccaggc
     2701 gggggagcag gaccttcgcg tacacgtgca tcccgggggg ctcgggctcg atgaggcgga
     2761 tcttcatggc gctccttgga gggggagtga gagccctccg gcggagcggc cggctgtagc
     2821 gccgatccgt gtcacgcagg gcggtcacga gttgatgctg attgtcgcag ggtcgccccg
     2881 tggaacagcc gggcgggcgc tgcgcggacg tacaccgtcg gtggacagca taccgcatgc
     2941 gccccgggcg gaatcccggc gggacgttcc gcgcggactc gcgtcctaga agatcgcccg
     3001 gatcgcctcg ccggcgaggt agaccccggc gccgacgagg ccggccgccg gcaacgtgag
     3061 gatccacgcc gtgacgatgt tgccggcgac gccccagcgg acggcggaga ggcgctgcgt
     3121 ggagccgacg cccatgatgg ccgccgtgat cgtgtgcgtg gtggagacgg ggacgccgaa
     3181 gtgcgtggcc acctggatga cgctggcggc ggcggtctcg gcggcgaacc cgtggatggg
     3241 atcaagcttt atgaccttgg tccccagcgt gtggatgatg cgccagccgc cggagtaggt
     3301 gcccagggcc attgcgaggg cgcagacgat cttgacccac gtggggatcg tgaacgtgtc
     3361 gatctggccg ctggcgaaca gtgccagagc gatgatgccc atggtcttct gggcgtcgtt
     3421 ggcgccgtgc gagaaggcca tgaacccggc cgagatcggc tgcaggcggc ggaaccactt
     3481 gttgacgggc gccagctggc gtttgcgcac cagccagatg aggccggtca tgagcaccca
     3541 cgcgccgatg aagccgagga gcggcgagcc gatcatcggc aggatgatct tctcgaccac
     3601 gccgctccag atgacgccgt cgacgcccgc actggcgatc atggcgccga tgaggccgcc
     3661 gaagagcgcg tgcgaggagc tggaaggtat gccgaagtgc caggtggcga agttccagac
     3721 caccgctccg accagcgcgg ccatgacgac ctgaagggtg acgaggtcgg tctcgacgat
     3781 cccttcgccc accgtcttgg cgacgctggt cgagaccagc gcgccgagca ggttgagact
     3841 ggccgccatg agcaccgcgg tgcgaggaga gagggcgcgc gtcgacaccg aggtggcgat
     3901 cgcgttggcg gtgtcgtgga agccgttggt gtaatcgaag acgagcgcca gaatgacgat
     3961 cgcaatgagg aggaccacgg cctgtgctca ggtcagttgt gtttgagcac gatgctctcg
     4021 atgacgttgg ccacgtgttc gcactcgtcg atggccgcct cgaggatctc gtagatgtct
     4081 ttccacttga tgatgtcggt gcacttcatc tcgccgtcga acaggctcgc catggcgtcg
     4141 cgcaggatgc ggtcgccgtc gttctcgagg ctgttgatgg cgatgatgcg ctcgtcgagg
     4201 cccttgggct tctcgaggtt gtccatgcac tccttgagca gcggagtggc ccgggcgatg
     4261 acctccgcct ggcgcttggc ctgctcggtg atctcgccga cccggtacag catgaccatg
     4321 tcggtcgcgg cctcgatgtt gtcgaggatc tcgtcggtgg tcgccacgag gtcatagatg
     4381 tcctcgcggt cgatcggcgt gatgaacgac ttgttgaggc ggcggatgat gtcgtggacg
     4441 atcttgtcgc cgacgtgctc gtgctcgagg atcgcggcgg cgatgctctt gcggtcgccg
     4501 tcgctcatga acctgggcag cagctgcgcg gcggcgtcga ggttcgccgc gagctctgag
     4561 aacagccgga agtagtcgtg ttccttggga acgagactga gtttcacacg ggctcctttc
     4621 ccctacctgc cctgtgccgc gcgtaatgct atacggaatg gggcggtgcg caaccccctc
     4681 gtcgcggccg gcgcgccccc cggacggccg cctgatcacc ggacggaccc cgttgccggg
     4741 ggcggctacg ggcggcatcg ccgccgccgt caagttttcc gacggcgccg tcgttaaggg
     4801 gagaaggagc caccgagccg gtggctcacg acccgcagga ccgaaggagg tcgtcgctgt
     4861 gaagaaagtg ctcctgatcg tcgtcgtcgc tgccctcgcg ctgacgctcg tgccgctggc
     4921 catcgccggc ggacacggcg gtgggggcca caagaacggc aagtacaagt tcaatctggt
     4981 cggcaacgtg gtcgcccccg acagcgatcc cgcggtcgaa ccggcggtcg accccgcggc
     5041 cgaccccgac gccgccccgg tcgtcgccga ccccactgaa ccggccgagc cggccgtcgt
     5101 ctcgaccatc ttcatcaagg tgaaggccgg cagccacatc aaggggcttc gcggcacgga
     5161 gaagatgttc gacgtggccc ccggcgtcaa ggtctggcag ctcaccgccg acggcgccgt
     5221 cgaggggagg ctcgccgaca tcgagcccga cgaccgggtc aaggtcaagg gctggatctc
     5281 gaaggacggc tcgaaggtct acacgatcac gagcatcaag taccgcgatc tcaccccggt
     5341 ggtggaagag accgccccgc cggccgcgcg gtagcgaaac gcacacacgc gggcgcgccc
     5401 gcgacagact gaaagcacgg ccgccggccg ggctcgccgg g
//
LOCUS       DASUMX010000115         9611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_606893, whole genome shotgun sequence.
ACCESSION   DASUMX010000115 DASUMX010000000
VERSION     DASUMX010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 9611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9611
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_606893"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            523..3231
                     /gene="cphA"
                     /locus_tag="VFZ86_05685"
     CDS             523..3231
                     /gene="cphA"
                     /locus_tag="VFZ86_05685"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: protein motif:HMM:NF010623.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEX5641817.1"
                     /translation="MVKLLDSRAIRGANYYSYRPVVVLNVDLGEYHEVFTDALPGFTD
                     ALLELVPSLEEHRCSENERGGLIKRMREGTLLGHVIEHLALELQYIAGMEVGFGKTID
                     TDTPGVYQVIYSYWVEEAGITAGERAIDIVNALLEGRRDDIDLEGVIRELEDIGADHY
                     LGPSTAAIVEEAEHRAITVLRLDDYNLVQLGEGKHQRRIEASITSRTSMIGVETAGNK
                     RLTKHMLEDAGIPVPKGTVVRRPESALEDAEWLGYPVVIKPHDGHHGKGVTTNISGEA
                     DLREAFARAKEISDKVIVEKCYTGNDYRMLVVDGRLVAAAMREPAAVTGDGEHTIEEL
                     IAIENRNPRRGYGHEKVMTRLSTSGVTQFLLERAGFTLETVLPEGEVFRLELTANLST
                     GGSAVDVTDTVHKANRFMAERIASIIGLDIAGIDVIAPTLEQPVKKVGGAVIEVNAAP
                     GLRMHLAPAEGTPRNVARPIVDMLFPPGAPHDIGIVAVTGTNGKTTTVRLIAHIMEHA
                     GYSVGMTTTDGIYVRGHLIAEGDMSGPYSAQVVLRDPLVDCAVLETARGGILRSGLGY
                     KTADVGVVLNVQPDHLGLQNIRDLDELAKVKAVVAEAVREGGTTVLNADDPMCVGMTE
                     YCREHVVFFSLRSTNPVVLEHVDQGHTAVVCEQGYIAVFGGDRLTPVARIEDIPLTLG
                     GLAEFNVQNALAATAAAWAHGVDVDEIRRALLSFLPSSTQTPGRLNRMSIAGVDYLLD
                     YAHNPHAYKGLVDLVQRMGEKRRTIVFDVVGDRRDKDIAEVCDLVAPAFDRAILYEAE
                     DRRGRAPGELMDLQEKCLTAAGFDAAAIERIGGEEGAIRRAVATAAPGELVCYMTGRV
                     QAGIRLLTALAEGESAAGAATAAGAAGDDTRRGTHAPGRHEAEGRA"
     gene            3228..4034
                     /locus_tag="VFZ86_05690"
     CDS             3228..4034
                     /locus_tag="VFZ86_05690"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009057 - macromolecule catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HEX5641818.1"
                     /translation="MTETRRGWRLLWGGSGPEGMDPRFVRRFVELAGGARGARIAVVP
                     TASEERADTIARYTESLRREDVRQLEVLDIRERAEADEPAMLAKLREATGVMLTGGDQ
                     LRLLDILGGTGFAAELLRRGREGLLVAGSSAGSMALGDPVIVRGDPAAFHEPGAIRHE
                     RGLGLLDGVTIDTHFMVRGRLGRLVAIVSEFPDVIGLGIDEDCGLLVAPDGVATVMGH
                     AVVCVVDAHAAEPQPAVAGEGRGLSVSGLALHVLAEGDRFDLGTRAVCRD"
     gene            4188..4796
                     /locus_tag="VFZ86_05695"
     CDS             4188..4796
                     /locus_tag="VFZ86_05695"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5641819.1"
                     /translation="MRLLIAHQSSSEREALAEAVGRLEQPLEIITSDNGLDTLDLLLQ
                     DEAPDVALVDWDLAGVEGPEMCRLVRDFHQGRRTYMVVLATAERSDTTDAWRAGANDC
                     LTTPAPAHAVRACVQKAYKDARPEAAQDGRKRGAHAGGAAMLDAVLCADSDAGNFFGF
                     GVDLGATPSDPAWADGAGPADAPEAPEARSRAALLQAVLSER"
     gene            complement(4895..5329)
                     /locus_tag="VFZ86_05700"
     CDS             complement(4895..5329)
                     /locus_tag="VFZ86_05700"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX5641820.1"
                     /translation="MTTIPQKLHDVLAGGHNIWVATTEPDGAPNVSIKGSGLLLEDGR
                     IVFADLYGLDTYENLRHDPRVAIGVHDYASKVAMEVRGHAEIVDEGELVEDMKQRLAG
                     IGEKWRLPAVKHVVCVTVDTVVDLWPGPHAGEEVREVQATAT"
     gene            5484..6344
                     /locus_tag="VFZ86_05705"
     CDS             5484..6344
                     /locus_tag="VFZ86_05705"
                     /inference="COORDINATES: protein motif:HMM:NF022441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HEX5641821.1"
                     /translation="MTLRRMRSVTQGEAPATGAVAAAVAVLAALLALALVAAGCDMPS
                     TGGLGQVSGSGTPVTKTYDFTGFTRVTADGGFTVTVTRGAQYAVSVTVDDNLVEEHLK
                     VELDGETLRIGLEPLWAYRDLTLEARVTMPGLTGLEASGASTVSATGFTSGDPLALTV
                     SGASALTLAGAGAGRVTLEVSGAGRLEGELEAQELAGEISGAGAVELRGSARRLELDA
                     SGGSRFELLSLPAVDAELRLSGGSRGAVTVTGTLSADVSGGSRLEYAGSPQLEGVDSS
                     GGSVVEPAGE"
     gene            complement(6387..8021)
                     /locus_tag="VFZ86_05710"
     CDS             complement(6387..8021)
                     /locus_tag="VFZ86_05710"
                     /inference="COORDINATES: protein motif:HMM:TIGR04275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641822.1"
                     /translation="MRSPFVLFLALTALSVAVLGTAAAAALAFPPYAPGWLLATGPSL
                     QQSAQNAGDFSVWEDDAAGDWDVVYGRPAAQTVLPAGTSAQRHPAVWGTVIVYEDDRG
                     GDWDLYAWQTDPALVPLGSGPESLVASGAGDQRDPAVSGSLVVYEDTSRGNVDIVLHD
                     LAAGVTRRLTGNTAPQADPSIDGVTVAWADKRNGDWDIYAFDLSANRLRRLTTSRADQ
                     TRPQVGQGRVVYQDRRNGHWDVYEYDLRSGRERRLTSDKHDQTAPWIDRDPAPGRTGT
                     VVYQDDRADAGDIYVREGRTGISKRVCGEAGAQTGPSYDAEHLAWSDARTGQPDVYGC
                     RLLFPSLSARASAAAPAYNSSVRVVGELSLPDDFTDVHVIKAVAGARSLTGKAVGDGD
                     ARAGFSIPLGRIQRKLTVKVWYPGSATQLPAGGGTVVFKPTALLSRPSLVKQKLSGIT
                     TRRFYTVSGTLRPRHAAGSKAVTLLVYRKSGVGVWKVYRTLRVAVRDSSTASSYRTQV
                     RLDRTYLTSYLIVAVHGDADHTRTESPQSRIIGVDD"
     gene            8117..8680
                     /locus_tag="VFZ86_05715"
     CDS             8117..8680
                     /locus_tag="VFZ86_05715"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018758087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5641823.1"
                     /translation="MSDERARIRPCTDADLKAMVAVVNAAATAYAGVIPADRYHEPYM
                     PREELCAEIAAGVRFWGLFEARGPRIGLSADDSALAGVMGVQDAGDVTLIRHAYVRPD
                     RQRTGVGGRLLAHLLGPADVLPARPLLVGTWAAAWWAVAFYERHGFVLVTPEEKDRLL
                     RRYWSIPERQVETSVVLRFAPVARVQR"
     gene            8761..9129
                     /locus_tag="VFZ86_05720"
     CDS             8761..9129
                     /locus_tag="VFZ86_05720"
                     /inference="COORDINATES: protein motif:HMM:NF023390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS/SEC14 domain-containing protein"
                     /protein_id="HEX5641824.1"
                     /translation="MIQLIEGAPEGVLAFEAVGEVDAEDYEHVLKPAIEESLAGSDQL
                     RFVFEIGAEFDRFTAGAEWQDMTLGFAHFSDWLRCALVTDLDWVRHAAKALGWLMGGR
                     LRVFEIDELKAALEWAAADD"
     gene            complement(9172..>9611)
                     /locus_tag="VFZ86_05725"
     CDS             complement(9172..>9611)
                     /locus_tag="VFZ86_05725"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5641825.1"
                     /translation="AQMRTMLLELRGEAVEDVPLKLLLRHLAEATQSRARVSVELSTA
                     GEASLPAHVHVATFRIAQEALNNVVRHAHAATAQVELELGSRGLRLTIADDGCGFDPG
                     CAVDPTHLGLVSMRERAAEVGGRLGVTSSPGCGTRVELEWKRR"
BASE COUNT         1303 a         3376 c         3604 g         1328 t
ORIGIN      
        1 cgaccctctc cgagcgccgc gacggccagg agctgctcgc cagtgcggac cgcgccatgt
       61 acgcggccaa ggacggcggc cgcgaccgcg ttgtgcgggc cgacacgctg caggaggcgc
      121 ggccgcgcct ggcgacgcag ctgtgaggcg ccgctgggga cggcactgtc ccgcagcgga
      181 gtggcgccac ccatgacgcc accggtgatg ccactcgtcg cgggacactg acagctccgg
      241 ccgtcccagg gcgaggtcgc gcgtgatccg tgcgagcacc ctgagcgctg cgcggactcg
      301 cacgatcggg cgtgctcttg gaagcgctct tgtaccttgg cacgtacgtc cgggcgtgcg
      361 acagcgtgcg gcactaccgt gcgcggtgcg tgcgctgccc tgagccggtc gaagcggtgg
      421 cggcgcggcg gcgtccgcgg cgcgtcggcc cgccgccggc gccgccgtgc gccggcggcg
      481 ctgttgtacg atggcacctt ccccgaggag ggagggacga gaatggtcaa gctgctcgac
      541 tcgcgcgcca tcaggggcgc caattactac agctaccgcc cggtcgtcgt gctcaacgtc
      601 gacctcggtg agtaccacga ggtgttcacg gacgccctgc cggggttcac cgacgcgctg
      661 ctcgagctcg tccccagcct cgaggagcac cgctgctcgg agaacgagcg cggcgggctc
      721 atcaagcgca tgcgcgaggg gacgctgctc ggtcacgtca tcgagcacct cgcgctggag
      781 ctgcagtaca tcgccggcat ggaggtcggg ttcggcaaga ccatcgacac cgataccccc
      841 ggggtgtatc aggtcatcta cagctactgg gtcgaggagg ccggcatcac ggccggggag
      901 cgcgcgatcg acatcgtcaa cgcgctcctc gagggccgcc gcgacgacat cgacctcgag
      961 ggcgtgatcc gcgagctcga ggacatcggc gcggatcact acctcggccc gtccacggcg
     1021 gcgatcgtcg aggaggcgga gcacagagcc atcaccgtgc tccgcctcga cgactacaac
     1081 ctcgtccagc tcggcgaggg caagcaccag cgccgcatcg aggcctccat cacctcgcgg
     1141 acgagcatga tcggcgtgga gacggccggc aacaagcggc tgaccaagca catgctggag
     1201 gacgccggga tccccgtgcc caagggcacg gtcgtccggc gtcccgagtc cgccctcgag
     1261 gacgccgagt ggctcggcta cccggtcgtc atcaagccgc acgacggtca ccacggcaag
     1321 ggcgtgacga ccaacatctc cggcgaggcg gacctgcgcg aggcgttcgc gcgcgccaag
     1381 gagatcagcg acaaggtcat cgtcgagaag tgctacacgg gcaacgacta ccgcatgctg
     1441 gtggtggacg ggcgcctggt ggccgccgcc atgcgcgagc cggcggccgt gaccggcgac
     1501 ggcgagcaca ccatcgagga gctcatcgcc atcgagaacc gcaacccgcg gcgcgggtac
     1561 gggcacgaga aggtgatgac ccggctctcg acctcagggg tgacgcagtt cctgctggag
     1621 cgggccggct tcacgcttga gaccgtgctg ccggagggcg aggtgttccg gctcgaattg
     1681 acggcgaacc tctcgacggg cggctcggcc gtggacgtga cggacacggt gcacaaggcc
     1741 aaccgcttca tggcggagcg catcgccagc atcatcgggc tggacatcgc cggcatcgac
     1801 gtgatcgcgc cgacgctgga gcagcccgtc aagaaggtcg gcggcgcggt gatcgaggtg
     1861 aacgcggcgc cggggctgcg catgcatctg gccccggcgg agggcacgcc gcgcaacgtc
     1921 gcgcgcccga tcgtggacat gctcttcccg ccgggcgcgc cgcacgacat cggcatcgtc
     1981 gccgtcaccg gcaccaacgg caagacgacg acggtccggc tgatcgccca catcatggag
     2041 cacgcgggct acagcgtggg catgacgacc accgacggca tctacgtgcg cgggcacctg
     2101 atcgccgagg gcgacatgag cggcccgtac tcggcgcagg tggtgctgcg cgacccgctc
     2161 gtggactgtg ccgtgctgga gacggcccga ggcgggatcc tgcgctcggg cctcggctac
     2221 aagaccgccg acgtcggcgt ggtgctcaac gtgcagcccg accacctcgg cctgcagaac
     2281 atccgcgacc tggacgagct cgccaaggtc aaggcggtgg tggccgaggc cgtgcgcgag
     2341 ggcggcacca cggtgctcaa cgccgacgac cccatgtgcg tggggatgac ggagtactgc
     2401 cgcgagcacg tggtcttctt ctcgctgcgc tcgaccaatc ccgtggtgct ggagcacgtc
     2461 gaccagggcc acacggcggt ggtctgcgag caggggtaca tcgccgtgtt cgggggcgac
     2521 aggctgacgc cggtggcgcg catcgaggac atcccgctca cgctgggcgg actggcggag
     2581 ttcaacgtgc agaacgcgct ggcggcgacg gccgccgcgt gggcgcacgg cgtggacgtg
     2641 gacgagatcc gccgggcgct gctctcgttc ctgccgtcgt cgacgcagac gccgggcagg
     2701 ctcaacagga tgagcatcgc cggcgtggac tacctgctgg actacgcgca caacccgcac
     2761 gcgtacaagg gcctggtcga cctggtgcag cggatggggg agaagcggcg gacgatcgtg
     2821 ttcgacgtgg tcggcgaccg ccgcgacaag gacatcgccg aggtctgtga cctggtggcg
     2881 ccggcgttcg acagggcgat cctgtacgag gccgaggacc ggcgcgggcg cgcgcccggc
     2941 gagctgatgg acctgcagga gaagtgcctc acggcggccg gcttcgacgc cgccgccatc
     3001 gagcggatcg gtggggaaga gggcgcgatc cgccgggcgg tcgccacggc cgcgcccggc
     3061 gagctggtgt gctacatgac cggccgcgtg caggcgggca tccggctgct gaccgcgctg
     3121 gcggaaggcg agagtgcggc gggcgcggca acagccgccg gcgccgccgg cgacgacact
     3181 cgccgcggca cacatgcccc cggccgccac gaagccgaag gccgcgcgtg acggagaccc
     3241 ggcgcgggtg gcggctgctg tggggcgggt ccggcccgga gggcatggac ccgcggttcg
     3301 tgcgccgctt cgtcgaactg gcgggcggcg cgcgcggagc gcgcatcgcg gtcgtcccga
     3361 ccgcgagcga ggagcgcgcc gacaccatcg cccggtacac ggagtcgctg cggcgcgagg
     3421 acgtccgcca gctcgaggtc ctggacatcc gcgagcgggc ggaagcggac gagcccgcga
     3481 tgctcgcgaa gctcagagag gccaccggcg tgatgctcac cggtggcgac cagctgcgcc
     3541 tgctcgacat cctcgggggg accgggttcg cggccgaact gttgcgccgc ggccgcgagg
     3601 gcctgctcgt cgcaggctcc agcgccggct cgatggcgct cggcgacccc gtgatcgtgc
     3661 gcggcgaccc cgccgcgttc cacgagcccg gcgcgatccg gcacgagcgg ggcctgggcc
     3721 tcctcgacgg cgtcaccatc gacacgcact tcatggtgcg cggccgcctc ggccgcctcg
     3781 tcgccatcgt ctcggagttc ccggatgtga tcgggctggg catcgacgag gactgcggcc
     3841 tgctcgtcgc gcccgacggc gtggcgacgg tgatgggcca cgccgtggtc tgcgtcgtgg
     3901 acgcccacgc cgcggagccg cagccggccg tggccgggga ggggcgaggc ctctccgtca
     3961 gcggcctcgc cctgcacgtg ctcgccgagg gcgaccgctt cgacctcggg acgcgggcgg
     4021 tgtgccggga ctgagctcct cgggcggccg cggccgtccc gttgcccatc gtgaccacag
     4081 cttgtcgggc accgctggac gaacgtccta tgtcagcacc ggcgcgacgc gacgacgcgg
     4141 gcagtgaaga tggaactgcc cagtcgggct cgagccggga gaccaggatg cgactcctca
     4201 tcgcacatca gagcagcagt gagcgcgaag cgctcgccga agctgtcggc cggctggaac
     4261 agcctctcga gatcatcacg tcggacaacg gcctcgacac gctcgacctg ctcctgcagg
     4321 acgaggcgcc ggacgtggcc ctggtggact gggacctcgc cggggtggag ggccccgaga
     4381 tgtgccgcct ggtccgcgac ttccaccagg gccgccgcac ctacatggtc gtcctcgcca
     4441 ccgcggagcg ctcggacacg accgacgcgt ggcgggccgg cgcgaacgac tgcctcacca
     4501 cgccggcccc ggcgcacgcc gtccgcgcgt gtgtgcagaa ggcgtacaag gacgcccgcc
     4561 cggaggcggc gcaagacggc aggaagcgcg gcgcgcacgc gggcggcgcc gccatgctgg
     4621 acgccgtgct ctgcgccgac tccgacgcgg gcaacttctt cggcttcggc gttgatctcg
     4681 gtgccacacc gtcggacccc gcgtgggcgg acggcgccgg gccggccgat gcgccggaag
     4741 cgccggaagc acggagccgc gccgcgttgc tgcaggcggt cctgagcgag cgctgatcgc
     4801 tcgtcgcggc cgccctcggg cgggccgccg aagaggcggg ccgcggccgc cttgccgatc
     4861 gtcgccgcgg cccgcccctc cggcagccgg acgctcaggt cgcggtcgcc tggacctccc
     4921 tgacctcttc gcccgcgtgc ggccccggcc agaggtccac caccgtgtcg accgtcacgc
     4981 acaccacgtg cttgaccgcc ggcagccgcc acttctcgcc gatgccggcc aggcgctgct
     5041 tcatgtcctc gaccagctcg ccctcatcga cgatctccgc gtgccccctg acctccatcg
     5101 cgaccttgct cgcgtagtcg tgcaccccga tcgccacgcg cgggtcgtgt cgcaggttct
     5161 cgtaggtgtc caggccgtag aggtcggcga agacgatgcg gccgtcctcc agcagcagcc
     5221 cgctgccctt gatggagacg ttgggcgcac cgtccggctc ggtggtcgcc acccagatgt
     5281 tgtgcccgcc ggcgagcacg tcgtggagct tctgagggat ggtcgtcatg gttcccctcc
     5341 tcgtctcgct gtcccacttg agaaggttcc cggcaaggag cctgccggac actgttgacc
     5401 cggcggtgcg ggcggggcgc tgctatcatc gcggtcgggc gtgggcccgt tgggcgctgg
     5461 gctgtgcgag agaggaggcc ggcgtgacgc tccgccgcat gcgatcggtc acgcagggtg
     5521 aggcgccggc gaccggcgcg gtcgccgccg cggtcgcagt cctggccgca ctcctcgcac
     5581 tcgcgctggt ggccgccggc tgcgacatgc cgagcaccgg cggcctcggc caggtgagcg
     5641 gctcgggcac gccggtgacg aagacgtatg acttcaccgg tttcaccagg gtcaccgccg
     5701 acggcggctt cacggtgacg gtcacgcggg gcgcgcagta cgcggtctcg gtgaccgtgg
     5761 acgacaacct cgtcgaggag catctcaagg tggagctcga cggagagacg ctgcgcatcg
     5821 gtctggagcc gctgtgggcg taccgcgacc tgacgctcga ggcgagggtg accatgccgg
     5881 ggctcacggg gctcgaggcg agcggggcgt cgaccgtgag cgcgaccggg ttcacctccg
     5941 gcgacccgct cgccctgacc gtctccgggg ccagtgcgct gacgctggcc ggcgcgggcg
     6001 ccggccgggt cactctcgag gtctccggcg ccggccggct cgagggcgag ctcgaggcgc
     6061 aggagctcgc cggcgagatc tcgggcgccg gcgcggtgga gctgcgcgga tcggcccgga
     6121 ggctcgagct cgacgcctcc ggcggcagcc gcttcgagct cctgagcctg ccggccgtgg
     6181 acgccgagct ccggctctcc ggcggctcgc gcggcgcggt caccgtgacg ggcacgctga
     6241 gcgcggacgt cagcggcggc tcgcgtctcg agtacgccgg ctcgccgcag ctcgagggcg
     6301 tcgacagttc cggcgggtcg gtcgtcgagc ctgccggcga gtagcggtcc tctcggccgt
     6361 gggcgccggc ggcccgtgcc gcccgatcag tcgtccacgc cgatgatgcg gctctgcggc
     6421 gactccgtgc gcgtgtggtc cgcgtcgccg tgtacggcga cgatgaggta gctcgtgaga
     6481 tacgtcctgt ccaggcggac ctgggtgcga tacgagctcg cggtgctgga gtcgcggacc
     6541 gcgaccctga gcgtgcggta gaccttccag acgccgacgc cggacttgcg gtacacgagc
     6601 agcgtgacgg ccttgctgcc ggcggcgtgc cgcggccgga gcgtcccgct cacggtgtag
     6661 aagcgtctcg tggtgatccc ggacagcttc tgcttgacca gggaggggcg gctgaggagg
     6721 gcggtgggct tgaacaccac ggtgccgccc ccggccggca gctgcgtcgc cgacccgggg
     6781 taccagacct tcaccgtcag cttgcgctgg atgcgaccca gggggatgga gaagccggcc
     6841 ctggcgtcgc cgtccccgac cgccttgccg gtcaggctgc gggcgccggc gaccgccttg
     6901 atcacgtgga cgtcggtgaa gtcgtccggc agactcagct cccccaccac gcggacggag
     6961 ctgttgtagg cgggggcggc ggcggaggcc cgggcggaca ggctggggaa gagcagccgg
     7021 cagccgtaga cgtccggctg cccggtgcgc gcgtccgacc acgccaggtg ctcggcgtcg
     7081 tagctgggcc cggtctgcgc tccggcctcg ccgcacacgc gcttgctgat gccggtgcgg
     7141 ccctcacgga cgtagatgtc gccggcgtcg gcgcggtcgt cctggtacac caccgtgccg
     7201 gtgcgcccgg gcgccgggtc gcggtcgatc cagggcgccg tctggtcgtg cttgtcgctg
     7261 gtcagacggc gctccctgcc gctgcgcagg tcgtactcgt agacgtccca gtgcccgttg
     7321 cgacggtcct ggtagacgac cctgccctgg ccgacctgcg gcctggtctg gtcggccctg
     7381 ctcgtggtga gccggcggag ccggttcgcg gagaggtcga aggcgtagat gtcccagtcg
     7441 ccgttgcgct tgtcggccca cgcgacggtg acgccgtcga tggaaggatc ggcctggggg
     7501 gccgtgttgc ccgtcaggcg acgcgtcacg ccggcggcga ggtcgtgcag gacgatgtcg
     7561 acgttgccgc ggctcgtgtc ctcgtagacg acgaggctgc cgctgaccgc ggggtcgcgc
     7621 tggtcgccgg cgccggaggc caccagcgac tcggggcctg agcccagcgg gaccagggcg
     7681 gggtcggtct gccacgcgta caggtcccag tcgccgccgc ggtcgtcctc gtacacgatg
     7741 accgtgcccc agacggccgg gtggcgctgc gcgctcgtcc cggccgggag cacggtctgc
     7801 gcggccggcc ggccgtagac gacgtcccag tcgccggccg cgtcgtcctc ccacacgctg
     7861 aagtcgccgg cgttctgcgc cgactgctgc agcgagggac cggtcgcgag cagccatccg
     7921 ggggcgtacg gcgggaacgc gagcgccgcc gcggcggcgg tccccaggac ggcgacactc
     7981 agcgccgtga gtgcgagaaa caggacgaat ggactgcgca tcgcggccct ccagctggct
     8041 gacatcgggt ccatgttatc aggctcgtag agccggcttc tcccgcgctc gccgcgcatc
     8101 cgctagcatc ttgagcgtga gcgacgagcg cgcaaggatc cgcccctgca ccgacgccga
     8161 cctcaaggcg atggtcgccg tcgtcaacgc cgcggcgacc gcctacgcag gcgtgatccc
     8221 ggccgaccgg taccacgagc cgtacatgcc gcgggaggaa ctctgcgccg agatcgccgc
     8281 gggggtgcgc ttctgggggt tgttcgaggc ccgtggtccc cggatcgggc tcagcgccga
     8341 cgattcggcg ctcgcaggcg tcatgggcgt ccaggatgcc ggtgacgtga ccctgatccg
     8401 gcacgcctac gtgcggccgg accggcagcg cacgggcgtg ggtggccgcc tgctcgcgca
     8461 tctgctgggg ccggccgacg tgctgcccgc gcggccgctc ctcgtcggca cctgggcggc
     8521 cgcgtggtgg gccgtggcct tctacgagcg ccacggcttc gtcctcgtca cgccggagga
     8581 gaaggaccgc ctgctgcgcc gctactggag catcccggag cggcaggtgg agacgtcggt
     8641 ggtgctgcgc ttcgcgcccg tcgcccgtgt ccagcgctga gccgtcatcg gaggggctcg
     8701 tggtaccgtc ggtccagtgg atggggcgcg ccgcgccccc acggaggcag gaggtccggc
     8761 gtgatccagc tcatcgaggg tgctcccgag ggggtgctgg cgttcgaggc cgtgggcgag
     8821 gtggacgcgg aggactacga gcacgtgctc aagccggcca tcgaggagtc gctggccggc
     8881 agcgatcagt tgcgcttcgt cttcgagatc ggcgccgagt tcgaccgctt caccgcagga
     8941 gcggaatggc aggacatgac cctggggttc gcccacttca gcgactggct gcgctgcgcc
     9001 ctggtcaccg acctcgactg ggtgcggcac gcggcgaagg cgctgggctg gctcatgggc
     9061 gggcggcttc gggtcttcga gatcgacgag ctcaaggcgg ctctggagtg ggcggcggcg
     9121 gacgactgac cggggcgccg ccgccccgcg ctgctcacct gaggcggtcg ctcagcggcg
     9181 cttccactcg agctcgacgc gggtgccgca gcccgggctg ctggtcacgc cgagccggcc
     9241 gccgacctcc gccgcgcgct cacgcatgga cacgaggccg aggtgggtcg ggtcgacggc
     9301 gcagccgggg tcgaagccgc agccgtcgtc ggcgatcgtc agccgcaggc cccgggaccc
     9361 gagctcgagc tccacctgcg cggtggccgc gtgggcgtgg cgcacgacgt tgttcagcgc
     9421 ttcttgcgcg atgcggaatg tcgcgacgtg cacgtgcgcc gggaggctcg cctctcccgc
     9481 cgtcgagagc tctacgctca cccgggcccg gctctgggtc gcctcggcca ggtggcgcag
     9541 cagcagcttc aggggtacgt cctcgaccgc ctctccgcgc agctccagca gcatcgtccg
     9601 catctgcgcc a
//
LOCUS       DASUMX010000116        18951 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_607169, whole genome shotgun sequence.
ACCESSION   DASUMX010000116 DASUMX010000000
VERSION     DASUMX010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 18951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18951
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_607169"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VFZ86_05730"
     CDS             <1..190
                     /locus_tag="VFZ86_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="methyltetrahydrofolate cobalamin
                     methyltransferase"
                     /protein_id="HEX5641826.1"
                     /translation="LPNRGLLNRTFVAMCAGLGLDGLIVDVRNKQMMATIKSIEALRG
                     EDNFCGSYLKAHRAGILE"
     gene            195..1307
                     /locus_tag="VFZ86_05735"
     CDS             195..1307
                     /locus_tag="VFZ86_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEX5641827.1"
                     /translation="MTAPERCRLRDLGVAVGRLPAGPLNAITDVPGVRVGHCTVSWGG
                     PELPRGSGPARTGVTAILPHAGDLWHQRVVAGGHAANGVGEIIGLSAVREWGLVETPV
                     LLTNSQSAGAVYDATVRWLMEREPLAGVEDAVMPVVGECDDGFLNDLRGLHVREEHVR
                     AALDTAAAGPVAEGCVGAGTGMTCYDFKAGIGTSSRLVTAHGRPWTLGVLALTNFGVR
                     HRLSIDGVPVGREIADLMPLENREGSCIVVLATDAPLSSRQCGRLAKRCALGLAATGS
                     YASDGSGEIMLAFTTAHRVPRTSAEPLVLASITNDQMTELFEAAVDATAESVCNALTA
                     ATTTVGRDGNTAYALPIDRLVGALRRAGREARLPGA"
     gene            1632..2600
                     /locus_tag="VFZ86_05740"
     CDS             1632..2600
                     /locus_tag="VFZ86_05740"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5641828.1"
                     /translation="MDKRRYFIRKLLNALLTIILVASFNFALFRILPGDPARLLLPKG
                     KTDPSVIERQRKTFNLDKPMWQQFIYYWRDTVQGEFGMSFSEKRPVTEVVAERIWPTV
                     LLVGVGTVFATVVGMIMGVFAGWRRNGTYDVVSTNLGMVLYAMPTFWFGLLMIMLFAT
                     KLRWFPTGRMEEPGVELSGFAAFQSLARHLFLPAFTFAIAYVGEYHLIMRSSITGVMK
                     EDFTLTARAKGLSENAVLWRHVVPNAMLPTVTIVMMNLGFVMSGAILAESVFNWPGLG
                     LLSFDSMQKRDYPVMQAVFLIASIAVIVANLIADIMYYYLDPRVQA"
     gene            2597..4135
                     /locus_tag="VFZ86_05745"
     CDS             2597..4135
                     /locus_tag="VFZ86_05745"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5641829.1"
                     /translation="MIPRRFRNWVIFWTIVTWIVLELLTYPKEVPITSGFSAVIKGFF
                     SASVGAYPAWVMLLLAAICFVAGIIGLARKSKARVMNVLLLVEGAVFLIWGVLAGGGF
                     GAFSGSSNEISYNWAIQTVVVVLGGACVWAWAAGNPERAELTTWNTHQVWTLYRANWQ
                     GMVGLGILVVFMFMALLAPFLADHALLDPNAQIADPDTGELFGPYHPPTTYYYNWFGT
                     DQVGQSVLAQFIWSSRISLMVGLLAAFMSTVLGAGIGIAAGFYGGWQGEGWMRLTDAF
                     LVIPWLPLAMVLAAAWGQNYWMIILIIGITSWPGTARVIRSDTLRVRELSFIERAKAI
                     GSKNGHIMTKHVLPNVMPLIFANLVLVVAIAILSETTLSFLGLGDPLNFSWGTMLHYA
                     WVNGAAGLPAWWYLLPPGIAIILVVLAFTFIGTAYDEVLDPKLRKREDSTGESRFTAR
                     GEPSVAVAGGAGLGGGGGIFTPGQQTDDAMPDFNKPAGGSIRPEGGGGWVGGHIADDE
                     KEGK"
     gene            4135..5214
                     /locus_tag="VFZ86_05750"
     CDS             4135..5214
                     /locus_tag="VFZ86_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013143420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5641830.1"
                     /translation="MARSATKAKPSSEKQPVERIEHAGLLDVRNLTTHFKLGVGDVKA
                     VQDVSFTVAPGESLGLAGESGCGKTTAALSLLKLLPENGRIVSGDVLFDGRNLAKRTE
                     YGMSKIRWKEISIIFQGAMNALNPVRETGKQIAEPIQLHEGLSEAKAMVRVRELFDLV
                     GINPKRVMDFPHQLSGGMRQRAMIAMALACNPKLVIGDEPTTALDVMVQAQILELIHR
                     LRTELDLSFILISHDLSVMAETCDKGVVMYAGEVVESGTTADIFNNATHPYTRQLIKA
                     FPNIHEKREMVSSIKGDPPDLIHPPAGCAFEPRCDVRIESCKTDHPGLTEVERGHFVR
                     CVHARSRRSDDFVQGDEAAPEQEGV"
     gene            5217..6254
                     /locus_tag="VFZ86_05755"
     CDS             5217..6254
                     /locus_tag="VFZ86_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012448618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5641831.1"
                     /translation="MATEQTERPDLKLAPETGDVLFDVRDLEVYFPIQQSFFKSMVAT
                     EKKYVKAVDHISFQIKKGEILALVGESGCGKTTTGRTLLRLEDATGGEIIYKGKPVQK
                     FSGKELREYRKKAQIIFQDPYNSINPKQTIFDIVAEPLEVNNICSSEREKEERVITAI
                     SEAGLRPATEYLYRYPHELSGGQRQRVCIAGATVLDPDVIVADEPVASLDVSIRNDIL
                     KLMVEEKEKLGVTYIFITHDLSLAWVISDRIAVMYLGRIVEIGETEEVVANPKHPYTK
                     ALISVIPVPDPLAKREHVILKGETPNPVDIPSGCRFHPRCPEAIDACKETDPEDVHIG
                     GDHYAACIRIT"
     gene            6340..8169
                     /locus_tag="VFZ86_05760"
     CDS             6340..8169
                     /locus_tag="VFZ86_05760"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5641832.1"
                     /translation="MGRTHAAKAPAAAPRRLLAALAGAAAVLAGAAALSVPLPAASSS
                     TPPDPLTYRIGTTEECDGVNPFAASSPVSRECLRLGYDFLTWYDADYRPAPDIAESWV
                     VSSDGTTWTFRVREGMSWHDGVPLTAHDVAFTYGLLLETREPAYSHHLAGVKSVTAPD
                     DRTVVIVTRRPRADLLGIAVPILPEHVWKDVDRDALDLAGTRPFIGSGPFRIQEVQSG
                     RVRLVANDAYPDALGGRPALDVLDFVVERDPAALVAAYRAGSLDAIAGYPPAYAAALD
                     DAPGTTTVAAPALGRHELAFNCWSSRRSGGSPLLLDPSVRRAVHWAVDVEKLVAAATS
                     GLAVPGTSLLSPTQIGWRWEVPEAGRYRYDPLRAKRILEDAGYSDRDGDGVREDAADR
                     PLSFRLAALTGHPEHEVAARMIVRWCRDVGIDFRLEVRDDAGVTGDLRSGADFDAVIL
                     GRDGHVDPGFALGAFTTARIDEGSETRYSDPLYDSLYAAQREALDPADPGDTTRRRTI
                     TDAMQKLLYRDDPSIVLWYEVSLQAYRTDAWTGYAPAPKGAGAPFWNQLRATYLALRP
                     LPPAAPEPPGPAVVPWVVAGLLAAAGAAGVLWLRRRRKPGGAG"
     gene            8210..9685
                     /locus_tag="VFZ86_05765"
     CDS             8210..9685
                     /locus_tag="VFZ86_05765"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HEX5641833.1"
                     /translation="MTELAALWPDEAIEAMHLAALGLLERAGVRVESASARDLLLAAG
                     CAPLGEDRLTIPRRAVEEALAACPRAYTLAARDPERALLMDPDPGQTYVHNLGGARDV
                     SDARTGAGRRATLRDQVLATRVMHHLVHQEQVTSLWQPEDVPDLLEPLYSYLILARET
                     DKAIGGPGISYPFQALYLQEMACAVTGADGEGGVYPIDLAFSPVSPLILGGDVADALV
                     EQVRRGGAAVEILPCPAAATTAPGALSAALTQQHAEVLAGVVLTQAAAPGTPVYYGPR
                     LSAVDPRSGVVVSGTPETGVASVAAVLLARRCGLACDCYGPCSDSKVVDAQFGYERSV
                     NALLGLAARPRLLSGVGEIQAGVASCLEVLVIDDEVLNNARYALTPRPWDADALDLDA
                     MVDGVLSGRGFLGARHTRRYIRSEFVSPLLSYRGGLGDWLASGRAGLVDLASDRAAEL
                     AGRDPLGMPEHILDELVALIDRCAAGLGISGHPDPRRVLGL"
     gene            9695..10807
                     /locus_tag="VFZ86_05770"
     CDS             9695..10807
                     /locus_tag="VFZ86_05770"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family peptidase"
                     /protein_id="HEX5641834.1"
                     /translation="MAIPFIHVQARGSYRELGRAVGEAAREQVQASVDFFRANFSAMT
                     SGRLTFAAAEELAPAYAAAARRWLPRHVEELEGLAEGARVSFARLLVLNCGEEFTADE
                     PVTGGRALSPGAPRSGDHCTAVAVVAGGRHIVGHNMDWYVIDAVNNVLFDLTGPGGTR
                     IMGLAGAPYLLMLGMNSHGIGNVSNSVHSNDNRIGVPNIFVRRATLEARTLEEARDRG
                     LLAARARGTNQFFADAGGRLWDLETSATAHALIDHTGAGFMAHTNHYVSPDLRTFEGY
                     AGGESEVRLSTADRLLREGVAAGEDPLELVARVLRTHEPSPPEAICGHPAPDEAPAEQ
                     GMTVGSIICDLDERRLYACAGTPCDNAYRVFEMGGA"
     gene            10804..12453
                     /locus_tag="VFZ86_05775"
     CDS             10804..12453
                     /locus_tag="VFZ86_05775"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HEX5641835.1"
                     /translation="MTVHDLVVAGARVVDGCGNPWYLGDVGVRGDRIAAIAAPGTLRG
                     RRAVDAGGRYVTPGFIDPHTHSDLSILLHPRAETAVRQGVTTHVTGNCGMSAAPLTEK
                     HRADAVQNWAHYGWGLDGVAWDWRTFGQYLAAVHRGGHAINIAPLVGHGALRLAVVGF
                     AERAVTERELARMERLLDASMRAGAHGMSTGLVYPPGCFATTDELLALCRVVARHQGI
                     YTSHVRGERETILTAVAEALELGRAAGCPVEVSHNAPKWGAPEDASANLALIEEARRD
                     GLDATTDNDVHTDLAPRFSRALPQPVLDLSHEGLIALLSDPEARMALKRDVLEDALPG
                     AGYAGVVRHGRFDRVVVFHAGRHPEQRGRSVADIAAERGADAFDTFLDLIVENDDDIV
                     GLFDYIEEKNIRALLQSPIGMVCSDGLVMPPPDELDDPSLYWPCSYGEYPGVLERYVR
                     DEPVLRLEEAIRKMTSFPAQRFRLFDRGVLRPGLKADLVVFDLERVRDRATNLYPHTY
                     PFANIPHASPEGIDWVLVNGVPVLAEGEHTGDLPGRVLKRT"
     gene            12575..14224
                     /locus_tag="VFZ86_05780"
     CDS             12575..14224
                     /locus_tag="VFZ86_05780"
                     /inference="COORDINATES: protein motif:HMM:NF037653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HEX5641836.1"
                     /translation="MITVQDILEIPELDLRLLAGHKSTSNPVRWVHITEVPDPTRWLK
                     GGELLLTTGYSFVGDEEQQVAQIKRLIDHNISGLGFGTGFTFDKVPPALVKVAEEYGF
                     PLFEVPYHVPFLAITEAVASKIVNEQYSLLQRSLAVHEKLTKIVLEEKGLEAILSTLS
                     ALVGCSAVLFDFHGVVLCEAAYRRHLGAELVADLWRLIGDRRADRQNFALSMDGVGSG
                     VQVYPVVASHRIGAFLAVVKDSGDFSEYDRIILHNVVTVTALELVKKKAVAETEKRLA
                     GDFFDELIASDLYEEEIARRLAFFGLDPQSPHLIVLVGIDDAPDGAADERSEAATLDV
                     KERLHWAVDEFMARRDMLCISASRSDSVVILVQPGKIDAHEIIGLAGELQAVIAEMLP
                     EISVSVGIGRPHRQLIDLRQSYYEASYAIKIRKLKGEPRVIASFDDLGSYGLLLGLQD
                     TLSLEVFYDSVLGKLQEYDEQNSSDLVKSLACFLEANGHWGDAAEKLYVHRHTLRYRM
                     KRVEEITGRDLNQSQDRMEFWLALKAKELIDQSKKGKPASA"
     gene            complement(14261..14998)
                     /locus_tag="VFZ86_05785"
     CDS             complement(14261..14998)
                     /locus_tag="VFZ86_05785"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5641837.1"
                     /translation="MRVAGARVLLVGGVRRLGRELALDLGGHGAAVAVSSRAHTEAAD
                     QQVAALLAAGAHAAHWVEGDASRAAGAADLVARAEAVLGGLDALVYAASGPFIPRQPE
                     QIDEQTWDASMDTIAKGFFFSACAAKERLAAGGVIVALTDYLGLQPWATFAAHGAAKA
                     AEIHLVKQLARAWAPARVRVCGVAPGPVDLDDDEHREATLRAAARVAAERLVAPADIG
                     AAVRFCLETEGLTGVNIAVDNGSLVMS"
     gene            complement(14995..16005)
                     /locus_tag="VFZ86_05790"
     CDS             complement(14995..16005)
                     /locus_tag="VFZ86_05790"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX5641838.1"
                     /translation="MTEPASPHRAIAAIAAAVGGSVGIAARNLASGAEVHVAADELYP
                     MASCFKVPIMVEVMRQVDAGALRLGDRLTLSEADKSPGSTLIHCHEGLRPSVRDLLYL
                     MITLSDNTATDMLWRLVGLGSVNETMRRLGLGSIDCFMPNREYFLIESGAGEEWAGLS
                     GPEIVARWREIEARGDYEDAFRRVLEENARLSGAGFLHLYDRRWGRDGSCGYDDSFAV
                     DQALDNQGTPRDMAALLAMIVQGTCASAPSCRLMIEMMSRQEWRERIPAGLPDGLLVA
                     NKTGSVSGTSNDVAIVCAPTGVPMVMVVFWKGLAQGAKARADAAIAAIAAELYEHLGG
                     AA"
     gene            16216..17379
                     /locus_tag="VFZ86_05795"
     CDS             16216..17379
                     /locus_tag="VFZ86_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012662953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HEX5641839.1"
                     /translation="MIVGIPKEILEHEGRVAALPDEVAEYVKMGFTVLVETKAGDAAF
                     QSDEKYAAAGAEIVPDVADLWARADLVLKVKQPEPNKELGKHESELIGEGKMLITFLH
                     PANPSNHGYVRTLAERNVTSLTMDGIPRISRAQTMDALSSMSTITGYKSVIMGAYHLP
                     EFIPMIGTAIGATKPKSVLVIGTGVMGLQAVATAKRLGGVVKAFDIREAARAEAESLG
                     AKIAGFEVPQEIALGTGGYAQTLPAEWLEKEIEALKPLVADADLIILGALVPGEIAPV
                     LITEEMVKSMKPGSVIVDVSVDQGGNCACTQAGKEIFVDGVLVSAVANIPGSMPVVAS
                     WLYGKNMLQYVKNLFKNGPDATPDWDDDIVKNGLVTRDGKIVHHGTLVAMGQA"
     gene            17415..17705
                     /locus_tag="VFZ86_05800"
     CDS             17415..17705
                     /locus_tag="VFZ86_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012662954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HEX5641840.1"
                     /translation="MSKDVQSIIIMVVVFAASFGIGYTLISKVPQRLHTPLMSMTNAI
                     SAVTILGALLLFTTGDITSLELVLGAVALLTAGFNLVGGFAVTARMLKMFKR"
     gene            17796..>18951
                     /locus_tag="VFZ86_05805"
     CDS             17796..>18951
                     /locus_tag="VFZ86_05805"
                     /inference="COORDINATES: protein motif:HMM:NF014307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEX5641841.1"
                     /translation="MVEVTTLQKYVIELAIIVILIAGIAQFRSPAGARRGNYLAALAL
                     GLAIVFVLVSNDVRSWAVIVPSFLVGAVIGWIVAVRVNMIQIPALVAFQHGAGGVAAL
                     LVSFVELSRVSAELTPIGTISGYIGLIVGSATFAGSMIAAAKLANKIKQQPTVFKQHN
                     LILLLIIAVGVALTVGSAVTGGAAQQVLLLGLIAASMLLGVVFAIRIGGADMPVLISF
                     LNATAGLAAAFVGVVIESTLLIAAGATVASSGSILTWVMCTGMNRSLLNVLIGQKKTK
                     GGAAKPKAPEAVPAAPAPAAAAAPTDPKGRALAALEAAESVIVVPGYGMAQAHAAFEV
                     VRLADLLEKEGKQVRFAVHPVAGRMPGHMHVLLAEAEVEYEKLFEMQDIND"
BASE COUNT         2841 a         6957 c         6377 g         2776 t
ORIGIN      
        1 tctcccgaac cgtggcctgc tcaaccgcac cttcgtggcc atgtgcgcgg gcctcggcct
       61 cgacggcctg atcgtcgacg tgcgcaacaa gcagatgatg gccaccatca agtccatcga
      121 ggccctccgc ggcgaggaca acttctgcgg ctcgtacctc aaggctcacc gcgccggcat
      181 cctggagtaa cagcgtgacc gcgccggagc gctgccgcct gcgcgacctc ggcgtcgccg
      241 tcggcaggct gccggccggg ccgctcaacg cgatcaccga cgtgcccggc gtgcgcgtcg
      301 gtcactgcac cgtatcgtgg ggcgggcccg agctgccgcg cggcagcggg cccgcccgca
      361 cgggcgtcac cgcgatcctc ccgcacgccg gcgacctgtg gcatcagcgc gtcgtcgccg
      421 gcggccacgc cgccaacggc gtcggcgaga tcatcggcct cagcgccgtc cgcgagtggg
      481 ggctcgtcga gacgccggtc ctcctcacca actcgcagag cgccggcgcg gtctacgacg
      541 cgaccgtccg ctggctcatg gagcgcgagc cgctggccgg cgtggaagac gccgtcatgc
      601 cggtcgtcgg tgagtgcgac gacgggttcc tcaacgacct gcgcggcctc cacgtgcgcg
      661 aagagcacgt ccgcgccgcg ctggacaccg ccgcggccgg ccccgtggcc gaaggctgcg
      721 tgggggccgg cacgggcatg acgtgctacg acttcaaggc gggcatcggc acatcgtccc
      781 gcctcgtcac cgcccacggc cgcccgtgga cccttggcgt gctggcgctc accaacttcg
      841 gcgtgcgcca ccggctgagc atcgacggcg tgccggtggg aagagagatc gccgacctca
      901 tgccgctcga gaaccgcgag ggctcgtgca tcgtcgtgct ggcgaccgac gcgccgctca
      961 gctcgcggca gtgcggccgg ctggccaagc gctgcgcgct cggactcgcc gccaccgggt
     1021 cgtacgcgtc ggacggcagc ggggagatca tgctcgcctt caccacggcg catcgtgtac
     1081 cgcgcacgtc cgccgagccg ctcgtcctcg cgtcgatcac gaacgaccag atgacggaac
     1141 tcttcgaggc ggccgtggac gccacggccg agagcgtgtg caacgcgctc accgcggcga
     1201 ccaccaccgt gggccgcgac ggcaacaccg cctacgcgct gccgatcgac cggctcgtcg
     1261 gcgcgctgcg gcgcgcaggg cgtgaggcgc ggctgcccgg ggcgtagctc gccttgccgc
     1321 gccgcgccgc gccgactatc ctgacttcgt agccgcccgg ccgaagcgct ccgcgcaccg
     1381 ccgggccgtc agggggggta gcacacggcg agacgcccgt catgccggca tgacctcacc
     1441 gccgcaggcg cgggtccccc gcgctgccgg cgggatgccg gacccacgcc gtctgacctc
     1501 cccgggatcc gtccaggggt catcctcgcc ggcgtccgtg ccgctgacgt caggggtccg
     1561 gcaaccacgg atgtgagaag ggggtaaggg tccgtccgcg tccggggccg gacgcgcgac
     1621 ccgagagtgc agtggacaaa cgacggtact tcatacggaa gctgctcaac gcgctcctca
     1681 ccatcatcct cgtcgcgtcg ttcaacttcg cgctcttccg catcctcccg ggcgacccgg
     1741 cccggctcct tctccccaag ggcaagaccg acccgtcggt catcgagcgc cagcgcaaga
     1801 cgttcaacct cgacaagccc atgtggcagc agttcatcta ctactggcgc gacaccgtgc
     1861 agggtgagtt cggcatgtcg ttctcggaga aacgcccggt gacggaggtc gtcgccgagc
     1921 gcatctggcc caccgtcctg ctcgtggggg tgggcaccgt gttcgccacc gtcgtcggca
     1981 tgatcatggg cgtcttcgcg gggtggagac gcaacggcac ctacgacgtc gtgtcgacga
     2041 acttagggat ggtgctctac gccatgccca ccttctggtt cggcctcctg atgatcatgc
     2101 tgttcgccac caagctccgc tggttcccta ccgggcgcat ggaggagccc ggggtggagc
     2161 tcagcggctt cgccgccttc caatcgctgg ccaggcacct gttcctaccg gcgttcacgt
     2221 tcgccatcgc ctacgtgggt gagtaccacc tcatcatgcg cagctccatc accggcgtga
     2281 tgaaggagga cttcaccctc acggcccggg ccaagggcct cagcgagaac gccgtgctct
     2341 ggaggcacgt ggtacccaac gcgatgctgc cgacggtcac gatcgtgatg atgaacctcg
     2401 gcttcgtgat gtccggcgcg atcctcgcgg agagcgtgtt caactggccc gggctggggc
     2461 tgctctcctt cgactcgatg cagaagcgcg actaccccgt gatgcaggcc gtgttcctca
     2521 tcgccagcat cgccgtgatc gtcgccaacc tcatcgccga catcatgtac tactacctcg
     2581 acccaagggt gcaggcatga tcccccggcg cttccggaac tgggtcatct tctggacgat
     2641 cgtcacctgg atcgtcctcg agctgctcac gtatcccaag gaggtcccca tcaccagcgg
     2701 cttcagcgcg gtgatcaagg gtttcttctc ggccagcgtc ggcgcctacc cggcgtgggt
     2761 catgctgctg ctcgcggcca tctgcttcgt cgccggcatc atcggcctcg cgcgcaagag
     2821 caaggccagg gtcatgaacg tgctgcttct ggtcgagggc gcggtgttcc tgatctgggg
     2881 cgtgctcgcc ggcggcggct tcggtgcctt ctcgggcagc tccaacgaga tctcgtacaa
     2941 ctgggccatc cagacggtcg tggtcgtgct cggcggggcc tgcgtctggg catgggcggc
     3001 cggcaacccc gagcgcgcgg agctgaccac ctggaacacc caccaggtgt ggacgctcta
     3061 cagggccaac tggcagggga tggtcggcct cggcatcctc gtggtgttca tgttcatggc
     3121 gttgctcgcg ccgttcctcg ccgaccacgc gctgctcgac cccaacgcgc agatcgcgga
     3181 ccccgacacc ggcgagctct tcggcccgta ccacccgccg accacgtatt actacaactg
     3241 gttcgggacc gaccaggtgg gccagagcgt gctcgcccag ttcatctgga gttcgcgcat
     3301 ctcgctcatg gtcggtctgc tcgccgcctt catgtccacc gtcctcggcg ccggaatcgg
     3361 catcgccgcc ggcttctacg ggggctggca gggtgagggc tggatgcggt tgacggacgc
     3421 gttcctggtc atcccctggc tgccgctggc gatggtgctc gccgccgcct ggggccagaa
     3481 ctactggatg atcatcctca tcatcggcat caccagctgg ccggggacgg cgcgcgtcat
     3541 ccgctccgac actctgcgcg tgcgcgagct gagcttcatc gagcgcgcca aggcgatcgg
     3601 ctccaagaac ggccacatca tgaccaagca cgtgctgccc aacgtgatgc cgctcatctt
     3661 cgccaacctg gtgctcgtcg tcgcgatcgc catcctgtcc gagacgacgc tgtcgttcct
     3721 cgggctcggc gacccgctca acttcagctg gggcaccatg ctccactacg cctgggtcaa
     3781 cggtgccgcc gggctgcccg cgtggtggta cctgctcccg ccgggcatcg ccatcatcct
     3841 cgtggtgctc gccttcacgt tcatcggcac cgcctacgac gaagtgctcg atcccaagct
     3901 gcgcaagcgc gaggacagca ccggggagag ccgcttcacc gcccgcggcg agccctcggt
     3961 ggccgtggcc ggcggcgccg gcctcggcgg cggcggcggg atcttcacgc cgggccagca
     4021 gaccgacgac gccatgcccg acttcaacaa gcccgccgga ggctccatcc gcccggaggg
     4081 cggcggcggc tgggtcggcg gtcacatcgc cgacgacgag aaggagggga agtgatggcc
     4141 aggtcggcga ccaaggcgaa gccctcctcc gagaagcagc ccgtcgagcg catcgagcac
     4201 gcggggctgc tcgacgtgcg caacctcacc acccacttca agctcggcgt cggcgacgtc
     4261 aaggccgtgc aggacgtgag cttcacggtc gcgccgggcg agagcctcgg cctcgccggc
     4321 gagtccggct gcggcaagac cacggcggcg ctgtccttgc tcaagctgct gcccgagaac
     4381 ggccgtatcg tctccggcga cgtgctcttc gacggccgca acctcgccaa gcgcaccgag
     4441 tacggcatga gcaagatccg ctggaaggag atcagcatca tcttccaggg ggcgatgaac
     4501 gcgctcaacc cggtgcgcga gaccggcaag cagatcgccg agcccatcca gctgcacgag
     4561 ggcctctcgg aggccaaggc gatggtccgc gtgcgcgagc tcttcgacct cgtcggcatc
     4621 aacccgaagc gggtgatgga cttcccgcac cagctcagcg gcggcatgcg ccagcgcgcc
     4681 atgatcgcca tggcgctggc ctgcaatccc aagctggtga tcggcgacga gcccaccacg
     4741 gccctcgacg tcatggtgca ggcgcagatc ctcgagctca tccaccggct gcgcaccgag
     4801 ctcgacctgt cgttcatcct catcagccac gacctctcgg tgatggccga gacctgcgac
     4861 aagggcgtcg tcatgtacgc gggcgaggtg gtcgagagcg gcaccacggc cgacatcttc
     4921 aacaacgcca cccaccccta cacgcgccag ctcatcaagg cgttccccaa catccacgag
     4981 aagcgcgaga tggtcagctc gatcaagggt gacccgccgg acctcatcca tccgcccgcg
     5041 ggctgcgcct tcgagccgcg ctgcgacgtg aggatcgaga gctgcaagac ggaccaccct
     5101 ggtctcacgg aggtcgagcg cgggcacttc gtgcgctgcg tccacgccag gagccggcgc
     5161 agtgatgact tcgtccaggg cgacgaagcc gcaccggagc aggaaggggt gtgacgatgg
     5221 ccacggagca gacagagcgt ccggacctga agctcgcgcc ggagaccggc gacgttttgt
     5281 tcgacgtcag ggacctcgag gtctacttcc ccatccagca gagcttcttc aagagcatgg
     5341 tcgcgacgga gaagaagtac gtgaaggcgg tcgaccacat cagcttccag atcaagaagg
     5401 gcgagatcct ggccctggtc ggggaatcgg ggtgcggcaa gacgacaacc ggccgcaccc
     5461 tcctcaggct cgaggacgcc accggcggcg agatcatcta caagggcaag ccggtgcaga
     5521 agttcagcgg caaggagctg cgcgagtacc gcaagaaggc gcagatcatc ttccaggacc
     5581 cctacaactc catcaatccc aagcagacga tcttcgacat cgtcgccgag ccgctcgagg
     5641 tcaacaacat ctgctcgagc gagcgtgaga aggaggagcg cgtcatcacc gccatcagcg
     5701 aggcggggct gcgcccggcg acggagtacc tgtaccgcta cccgcacgag ctctcgggcg
     5761 ggcagcggca gcgcgtctgc atcgccggcg ctaccgtgct cgacccggac gtgatcgtcg
     5821 ccgacgagcc ggtcgcctcc ctcgacgtct ccatccgcaa cgacatcctc aagctgatgg
     5881 tcgaggagaa ggagaagctc ggcgtcacct acatcttcat cacccacgac ctctcgctcg
     5941 cctgggtcat cagcgaccgc atcgccgtca tgtacctggg gcggatcgtc gagatcggcg
     6001 agaccgagga ggtcgtcgcc aatcccaagc acccgtacac caaggcgctg atcagcgtca
     6061 tcccggtgcc ggacccgctg gccaagcgcg agcacgtcat cctcaagggg gagaccccga
     6121 acccggtcga catcccctcc ggctgccgct tccacccgcg ctgcccggag gcgatcgacg
     6181 cctgcaagga gaccgacccc gaggacgtcc acatcggcgg cgaccactac gccgcctgca
     6241 tcaggatcac gtagcggaca agggccggcc ggggcggtga gccgcgaggc acgcaccgcc
     6301 ccggccggcc tgcgcatgag cagacggagg agccggcaga tgggacgcac tcacgcggcg
     6361 aaggcgccgg cggccgcgcc gcgccggctc ctcgccgcgc tcgccggcgc ggcggcggtg
     6421 ctcgccggcg ccgccgcgct gtccgtgccg cttcccgcgg cgtcctcctc cacgccgccg
     6481 gacccgctga cgtaccggat cgggaccacc gaggagtgcg acggggtgaa cccgttcgcc
     6541 gcgtcgagcc cggtctcccg cgagtgcctg cggctcgggt acgacttcct cacctggtac
     6601 gacgccgact accggccggc gccggacatc gcggagagct gggtggtctc gagcgacggg
     6661 accacctgga cgttccgcgt gcgcgagggc atgagctggc acgacggagt cccgctcacc
     6721 gcgcacgatg tggcgttcac ctacggcctg ctcctcgaga cgcgggagcc ggcctacagt
     6781 caccacctcg ccggcgtgaa gagcgtcacg gcgccggacg accgcacggt ggtgatcgtc
     6841 acccgccggc ccagggcgga cctgctcggt atcgccgtgc ccatcctccc cgagcacgtg
     6901 tggaaggacg tcgaccgcga cgccctcgac ctcgccggga cccggccctt catcggctcc
     6961 ggaccgttcc gcatccagga ggtgcagagc gggcgggtcc ggctggtggc gaacgacgcg
     7021 tatccggacg cgctcggcgg ccgcccggcg ctcgacgtgc tcgacttcgt cgtcgagcgc
     7081 gacccggccg cgctggtcgc cgcgtacagg gccgggtcgc tcgacgccat cgccggctac
     7141 ccgcccgcgt acgcggcggc cctcgacgac gcaccgggga cgaccaccgt cgccgcgccg
     7201 gcgctcggcc ggcacgagct ggcgttcaac tgctggagca gccgccggag cggtggcagc
     7261 cccctgctgc tcgacccctc ggtccgccgc gccgtgcact gggccgtcga cgtggagaag
     7321 ctcgtcgcgg cggcgacgtc cggcctcgcc gtccccggta cctcactgct ctcgccgacg
     7381 cagatcggct ggcgctggga ggtccccgag gccgggcgtt acaggtacga ccccctgcgc
     7441 gccaagcgga tcctggagga cgccggctac tccgatcgcg acggggacgg cgtgcgcgag
     7501 gatgcggcgg accgcccgct ctccttccgc ctggccgccc tcactgggca cccggagcat
     7561 gaagtggcgg cgcggatgat cgtccgctgg tgccgcgacg tagggatcga cttccggctc
     7621 gaggtccggg acgacgccgg cgttaccggc gacctgcgct ccggcgcgga cttcgatgcg
     7681 gtcatccttg gccgggacgg ccacgtcgac cccggcttcg ccctcggcgc cttcaccacc
     7741 gcgcgcatcg acgaggggag tgagacgcgc tactcggacc cgctgtacga cagcctctac
     7801 gccgcccaga gggaggcgct cgaccccgcc gacccgggcg acacgacgcg ccgcaggacc
     7861 atcaccgacg ccatgcagaa gctcctctac cgcgacgacc cctccatcgt cctgtggtac
     7921 gaggtgagcc tgcaggccta ccgcaccgac gcctggacgg ggtacgcgcc ggccccgaag
     7981 ggcgcgggcg cccccttctg gaaccagctt cgcgccacct acctggcgct gcggccgctg
     8041 cccccggcgg cgccggagcc gccgggcccc gcggtcgtgc cctgggtggt ggccgggctg
     8101 ctcgccgccg ccggcgccgc cggcgtcctg tggctgcgcc ggcggcggaa gccgggaggt
     8161 gcaggatgac cgcactcacc atcgcgacac cgacaggaga ggatgccgca tgaccgagct
     8221 cgccgctctc tggcccgacg aagcgatcga ggcgatgcac ctcgcggcgc tcggcctgct
     8281 cgagcgggcc ggcgtgcgcg tggagtcggc gagcgcgcgg gatctcctgc tcgccgccgg
     8341 ctgcgcgcct ctcggcgagg accggctcac gatcccccgg cgagccgtcg aggaggctct
     8401 cgccgcgtgc ccgcgcgcct acacgctcgc ggcgcgcgac cccgagcgcg ccctcctcat
     8461 ggaccccgac ccggggcaga cctacgtgca caacctcggg ggcgcccgcg acgtgagcga
     8521 cgcgcgcacg ggcgccggcc gccgcgccac gctgcgggat caggtgctgg ccacgcgcgt
     8581 catgcaccac ctcgtccacc aggagcaggt cacctcgctc tggcagccgg aggacgtgcc
     8641 cgatctgctc gagccgctgt actcgtacct catcctcgcg cgcgagaccg acaaggcgat
     8701 cggcgggccc gggatctcct atccgttcca ggcgctctac ctgcaggaga tggcgtgcgc
     8761 ggtgaccggg gccgacggcg agggcggcgt gtaccccatc gacctcgcgt tctcgccggt
     8821 cagcccgttg atcctcggcg gcgacgtcgc cgacgccctc gtcgagcagg ttcgcagggg
     8881 cggcgccgcc gtcgagatcc tgccctgccc ggccgccgcc acgacggcgc ccggcgcgct
     8941 ctccgcggcg ctgacgcagc agcacgccga agtgctcgcc ggggtggtgc tcacgcaggc
     9001 ggccgcgccc ggcacgccgg tgtactacgg gccgcggctc agcgccgtgg acccgcgcag
     9061 cggcgtcgtc gtcagtggca cacccgagac cggcgtcgcc tcggtggcgg cggtcctgct
     9121 ggcgcgccgc tgcggcctcg cctgcgactg ctacggaccc tgcagcgaca gcaaggtcgt
     9181 cgacgcgcag ttcggctacg agcgctcggt caacgcgctg ctcggcctgg cggccaggcc
     9241 gcgccttctc tccggcgtcg gcgagatcca ggccggcgtc gccagctgcc tggaggtgct
     9301 ggtcatcgac gacgaggtgc tgaacaacgc gcgctacgcc ctcacgccgc gcccctggga
     9361 cgcggacgcg ctcgacctgg acgcgatggt cgacggcgtg ctctccgggc gtgggttcct
     9421 cggcgccagg cacacccgcc ggtacatccg cagcgagttc gtcagcccgc tgctcagcta
     9481 ccgcggcggc ctcggcgact ggctcgcctc gggccgcgcc gggctcgtcg acctcgcctc
     9541 cgacagagcc gccgagctcg ccggccgcga tcctctcggc atgccggagc acatcctgga
     9601 cgagctcgta gcgctgatcg accggtgcgc cgccggcctc ggcatcagcg gccaccccga
     9661 cccgcgccgc gtgctcggcc tgtagactgc cgtcatggcc atcccgttca tccacgtcca
     9721 ggcgcggggc tcgtaccgcg agctcggccg cgccgtcggc gaggcggccc gcgagcaggt
     9781 gcaggcgtcg gtcgacttct tccgcgccaa cttctcggcc atgacgagcg ggcgcctcac
     9841 cttcgccgcg gcggaggagc tggctccggc ctacgccgcc gcggcgcgcc gctggctgcc
     9901 tcgtcacgtc gaggagctgg aggggctcgc cgagggcgcg cgcgtgtcgt tcgctcgtct
     9961 gctcgtgctc aattgcggcg aggagttcac cgccgacgag cccgtcaccg gcggccgcgc
    10021 gctctccccg ggtgcgcccc gctccggcga ccactgcacg gcggtcgccg tggtcgccgg
    10081 cggccgccac atcgtgggcc acaacatgga ctggtacgtc atcgacgcgg tcaacaacgt
    10141 cctcttcgac ctcaccggcc ccggcggcac gcggatcatg ggtctggccg gcgctccgta
    10201 cctgctcatg ctgggcatga acagccacgg catcggcaac gtgagcaact cggtgcactc
    10261 caacgacaac cggatcggcg tccccaacat cttcgtccgg cgagcgacgc tggaggcgcg
    10321 gacgctcgag gaggcgcggg accgcggctt gcttgccgcc cgcgcccgcg gcaccaacca
    10381 gttcttcgcc gacgccggcg ggcggctctg ggacctggag acgtcggcga ccgcccacgc
    10441 gctcatcgac cacaccggcg ccggcttcat ggcgcacacc aaccactacg tgtcgcccga
    10501 cctgcggacc ttcgagggct acgccggcgg cgagtccgag gtgaggctct ccacggccga
    10561 tcgtctgctg cgggaaggcg tcgccgccgg ggaggacccg ctggagctcg tggcccgggt
    10621 cctgcgcacg cacgagcctt ctccccccga ggcgatctgc ggccaccccg cgccggacga
    10681 ggcgccggcc gagcagggca tgacggtcgg cagcatcatc tgtgacctgg acgagcgccg
    10741 cctctacgcc tgcgcgggca cgccgtgcga caacgcctac cgggtcttcg agatgggtgg
    10801 cgcatgacgg tccacgacct ggtcgtcgcc ggcgcccgcg tcgtcgacgg ctgcggcaac
    10861 ccctggtacc tcggcgacgt cggcgtccgc ggcgaccgga tcgcggcgat cgccgcgccg
    10921 gggacgctgc gcggccggcg cgccgtcgac gccgggggtc gctacgtcac ccccggcttc
    10981 atcgatccgc acacgcactc cgacctctcc atcctgctgc acccgcgcgc cgagacggcg
    11041 gtccgccagg gcgtgaccac gcacgtcacc ggcaactgcg gcatgtcggc ggcgccgctc
    11101 accgagaagc accgcgccga cgccgtccag aactgggcgc actacggctg ggggctggac
    11161 ggcgtggcct gggactggcg caccttcggc cagtacctcg cggcggtgca ccgcggcggc
    11221 cacgcgatca acatcgcgcc gctcgtcggc cacggcgcgc tgcggctggc ggtcgtcggc
    11281 ttcgccgagc gcgccgtgac ggagcgcgag ctcgcgcgca tggagcggct cctggacgcc
    11341 agcatgcgcg ccggcgcgca cggcatgtcg accggcctcg tgtacccgcc cggctgcttc
    11401 gcgaccaccg acgaactgct cgcgctgtgc cgggtggtgg cgcgtcacca gggcatctac
    11461 acctcgcacg tgcgcggcga gcgtgagacg atcctcaccg ccgtcgccga ggcgctggaa
    11521 ctgggacgcg ccgccggctg tcccgtcgag gtctcgcaca acgcgcccaa gtggggcgcg
    11581 ccagaggatg cctccgccaa cctcgccctg atcgaggagg cgcggcgcga cggcctcgac
    11641 gcgaccacgg acaacgacgt gcacaccgac ctcgcgccac gcttctcgcg ggcgctgccg
    11701 cagccggtgc tcgatctcag ccacgaaggg ctgatcgcgc tgctgtcgga ccccgaggcg
    11761 cgcatggcgc tcaagcgcga cgtgctcgaa gacgccctgc cgggcgccgg atacgccggc
    11821 gtggtccggc acggccgctt cgaccgggtg gtcgtgttcc acgccggccg ccacccggag
    11881 cagcgaggcc gctccgtggc cgacatcgcc gccgagcgcg gcgccgacgc cttcgacacc
    11941 ttcctcgacc tcatcgtcga gaacgacgac gacatcgtcg ggctcttcga ctacatcgag
    12001 gagaagaaca tcagggcgtt gctgcagagc ccgatcggga tggtgtgctc ggacgggctg
    12061 gtcatgccgc caccggacga gctggacgac ccgtcgctct actggccctg cagctacggg
    12121 gagtacccgg gtgtgctcga gcgctacgtg cgcgatgaac cggtgctgcg cctcgaggag
    12181 gccatccgca agatgacctc cttcccggcg cagcgcttcc gcctcttcga ccggggggtg
    12241 ctgcgccccg gtctgaaggc cgacctcgtc gtcttcgacc tcgagcgggt gcgcgaccgg
    12301 gcgacgaacc tctacccgca cacgtatccc ttcgcgaaca tcccgcacgc ctcgcccgag
    12361 gggatcgact gggtgctggt caacggcgtg ccggtgctcg cggagggcga gcacacggga
    12421 gaccttcccg gccgcgtgct gaagcggacg tagggaccgc cgccgccggg agcgcgtgcg
    12481 acgtcccgtc cctgctccag aacgtacaag gccaccccgc ctcgaccgtc cgggaccctc
    12541 gcgctgtacg tggacacgat gtagaatccc ggcaatgatc accgtccagg acatcctcga
    12601 gatccccgag ctcgacctgc gactcctggc aggccacaag tccaccagca accccgtccg
    12661 atgggtccac atcaccgagg tccccgaccc caccaggtgg ctcaagggcg gagagctcct
    12721 gctcaccacc ggctacagct tcgtcggcga cgaggagcag caggtcgcgc agatcaagcg
    12781 cctcatcgac cacaacatct ccggcctcgg tttcggcacc ggcttcacgt tcgacaaggt
    12841 gccgcccgcg ctggtcaagg tcgccgagga gtacggcttc ccgctgttcg aggtgccgta
    12901 ccacgtgccg ttcctggcca tcaccgaggc ggtggcgtcc aagatcgtca acgagcagta
    12961 ctcactcctg cagcgcagcc tggccgtcca cgagaagctc accaagatcg tgctggagga
    13021 gaaaggcctc gaggccatcc tgtccaccct ctcggccctg gtcggctgct ccgccgtcct
    13081 gttcgacttc cacggcgtcg tgctctgcga ggcggcgtac cgccgccacc tgggcgccga
    13141 gctggtcgcc gacctgtggc ggctcatcgg cgaccgccgc gccgaccgcc agaacttcgc
    13201 cctcagcatg gacggcgtgg gctcgggcgt gcaggtctat ccggtggtcg cgtcgcaccg
    13261 catcggcgcg ttcctcgccg ttgtcaagga cagcggcgac ttcagcgaat acgaccgcat
    13321 catcctgcac aacgtggtca cggtcacggc gctggagctg gtcaagaaga aggccgtcgc
    13381 cgagacggag aagcggctcg ccggcgactt cttcgacgag ctcatcgcca gcgacctgta
    13441 cgaggaggag atcgcccggc ggctcgcctt cttcgggctc gacccgcagt cgccgcacct
    13501 catcgtgctc gtgggcatcg acgacgcgcc ggacggcgcg gcggacgagc gcagcgaggc
    13561 cgccacgctc gacgtcaagg agcggctgca ctgggccgtg gacgagttca tggccagacg
    13621 cgacatgctc tgcatcagcg cgtcgcgcag cgactccgtc gtcatcctcg tgcaacccgg
    13681 caagatcgac gcccacgaga tcatcgggct cgcgggggag ctgcaggcgg tcatcgcgga
    13741 gatgctgccg gagatctccg tgtccgtcgg catcgggcgg ccgcaccgcc agctcatcga
    13801 cctgcgccag agctactacg aggcgagcta cgccatcaag atccgcaagc tcaagggcga
    13861 gccgcgcgtc atcgcgagct tcgacgacct gggctcctac gggctgctgc tcgggctgca
    13921 ggacacgctg tcgctcgagg tcttctacga cagcgtgctc ggcaagctcc aggagtacga
    13981 cgagcagaac tcgagcgact tggtcaagag cctcgcctgc ttcctcgagg ccaacgggca
    14041 ctggggcgac gccgcggaga agctgtacgt gcaccggcac acgctgcgtt accgcatgaa
    14101 gcgggtggag gagatcaccg gccgcgacct caaccagtcg caggaccgca tggagttctg
    14161 gctggccctc aaggccaagg agctcatcga ccagtccaag aagggcaagc ccgcctccgc
    14221 gtgaccccgc cggccgccgg cgccggaccg gccggctccc tcaggacatg accagcgagc
    14281 cgttgtcgac ggcgatgttg acgccggtca gaccctctgt ctccaggcag aaccgaacgg
    14341 cggcgccgat gtcggcgggc gcgaccagcc gctccgcggc gacgcgcgcc gccgcgcgca
    14401 gggtcgcctc ccggtgctcg tcgtcgtcca ggtccaccgg gcccggcgcc acgccacaga
    14461 cgcgtacgcg cgcaggcgcc cacgcgcgcg cgagctgctt caccaggtgg atctcggccg
    14521 ccttggcggc cccgtgcgcg gcgaaggtgg cccacggctg cagcccgagg tagtcggtga
    14581 gcgcgacgat cacgccgccg gcggcgaggc gctccttggc ggcgcaggcg ctgaagaaga
    14641 accccttggc gatggtgtcc atggaggcgt cccacgtctg ctcgtcgatc tgctccggct
    14701 gccgagggat gaaggggccg ctggccgcgt acacgagcgc atcgagcccg ccaagcacgg
    14761 cctcggcccg ggcgacgagg tccgccgccc cggcggcgcg gctcgcgtcg ccctccaccc
    14821 agtgcgccgc gtgcgctccc gccgcgagca gagccgccac ctgctggtct gccgcctccg
    14881 tgtgcgcgcg cgacgagacg gctacggcgg cgccgtggcc gccgaggtcg agcgcgagct
    14941 cgcgcccaag gcggcgcacg ccgccgacca gcaggacgcg ggcgccggcg accctcacgc
    15001 tgctcccccg agatgctcgt acaactccgc cgcgatggcc gcgatggcgg cgtcagcccg
    15061 cgccttcgcg ccctgcgcga ggcccttcca gaacacgacc atgaccattg gtacccccgt
    15121 gggcgcgcag acgatcgcga cgtcgttgct cgttccgctg acgctgccgg tcttgttggc
    15181 cacgagcagc ccgtcgggca ggccggcggg gatcctctcg cgccactcct ggcggctcat
    15241 catctcgatc atgagccggc acgacggcgc cgacgcgcag gtgccctgca cgatcatcgc
    15301 cagcagggcg gccatgtctc gcggcgtgcc ctggttgtcg agggcctggt cgacggcgaa
    15361 ggagtcgtcg tagccgcacg acccgtcccg tccccagcga cggtcgtaca ggtgaaggaa
    15421 gccggcgccg ctgaggcgcg cgttctcctc gagcacgcgg cggaacgcgt cctcgtagtc
    15481 cccgcgcgcc tcgatctccc gccagcgggc gacgatctcg gggccgctga ggcccgccca
    15541 ctcctcgccg gcgccggatt cgatgaggaa gtactcgcgg ttgggcatga agcagtcgat
    15601 ggaccccagg ccgaggcgac gcatcgtctc gttgacggat ccgaggccga ccagccgcca
    15661 gagcatgtcg gtggccgtgt tgtcgctcag ggtgatcatc aggtagagga gatcgcgcac
    15721 gctcggacgc agtccttcgt ggcagtggat cagcgtgctc ccggggctct tgtcggcctc
    15781 gctcagcgtg agccggtcgc cgagtcgcag cgcacctgcg tcgacctgcc gcatgacctc
    15841 gaccatgatg gggaccttga agcagcttgc catgggatag agctcgtctg cggcgacgtg
    15901 cacctccgca ccgctggcca ggttgcgggc ggcgatcccg acgctgccgc ccacggcggc
    15961 ggcgatcgcg gcgatggcgc ggtgcggtga agccggctcg gtcatggctg ggccttcccg
    16021 gtcgattgcc ccaacgatac acgagggcgt ggaccgcggt catggggagc gcctcccgac
    16081 gttcggtcat gggcactttc tttctctttt gggcttttct gggcgcacgt atattgctag
    16141 actgccgtcg acacggagga cttcacgacg ccgggccgca tgcccgtcca tgggacaaga
    16201 atctgaggag gaacaatgat cgtcggtatc ccgaaggaga ttctcgagca cgaaggacgg
    16261 gtggccgccc ttcctgatga ggtagccgag tacgtcaaga tgggattcac ggtgctcgtc
    16321 gagaccaagg cgggagacgc cgcgttccag tccgacgaga agtacgcggc ggccggcgcc
    16381 gagatcgtgc ccgacgtcgc ggacctgtgg gcgcgcgccg acctcgtgct caaggtgaag
    16441 cagccggagc ccaacaagga gctcggcaag cacgagtccg agttgatcgg cgagggcaag
    16501 atgctcatca cgttcctgca tccggcgaac ccgtcgaacc acggctacgt ccgcacgctc
    16561 gccgagcgca acgtcacctc cctcaccatg gacggcatcc cgcgcatctc gcgcgcccag
    16621 accatggatg cgctcagctc gatgtcgacc atcaccggct acaagtcggt catcatgggg
    16681 gcgtaccacc tgcccgagtt catccccatg atcggcaccg ccatcggggc caccaagccg
    16741 aagtcggtcc tcgtcatcgg caccggtgtc atggggcttc aggccgtcgc caccgcgaag
    16801 cggctcggcg gcgtcgtcaa ggccttcgac atccgtgagg ctgcccgcgc tgaggccgag
    16861 agcctcggcg ccaagatcgc cggcttcgaa gtgccgcagg agatcgccct cggcacgggc
    16921 ggctacgcgc agacgctccc ggccgagtgg ctggagaagg agatcgaggc tctcaagccg
    16981 ctcgtcgccg acgccgacct gatcatcctc ggcgctctgg tcccgggaga gatcgccccg
    17041 gtgctgatca ccgaggagat ggtcaagtcc atgaagcccg gctccgtcat cgtcgacgtc
    17101 tccgtcgacc agggcggcaa ctgcgcctgc acccaggccg gcaaggagat cttcgtcgac
    17161 ggcgtcctcg tgtcggccgt ggccaacatc cccggttcga tgcccgtggt cgcgagctgg
    17221 ctctacggca agaacatgct gcagtacgtc aagaacctct tcaagaacgg ccccgacgcg
    17281 acgccggact gggacgacga catcgtcaag aacgggctgg tgacgcgcga cggcaagatc
    17341 gtgcatcacg gcacgctcgt tgccatgggc caggcctgac cgcgccgttc gcctgagaga
    17401 gaagggacgg cttcgtgagc aaggatgtcc agagcatcat catcatggtg gtcgtgttcg
    17461 ccgcgtcgtt cggcatcggg tacacgctga tctcgaaggt gccccagcgg ctgcacacgc
    17521 cgctcatgtc gatgacgaac gccatctcgg ccgtcaccat cctcggcgcc ctcctgctct
    17581 tcacgaccgg cgacatcacg tcgctcgagc tggtgctcgg cgcggtggcg ctgctcacgg
    17641 cgggcttcaa cctggtgggc gggttcgcgg tcaccgcccg catgttgaag atgttcaagc
    17701 gctgaagcga tcaagacagg gcctcggcgc cggtcacctg accggcgccg aggcttttct
    17761 cacgtcgacg cccgcggggg ccgggagcga ggaccatggt tgaggtaacc acgctgcaga
    17821 agtacgtcat cgagctggcg atcatcgtca tcctgatcgc cggcatcgcc cagttccgct
    17881 cgcccgcggg cgcgcggcgc ggcaattacc tggccgccct cgcgctcggg ctcgccatcg
    17941 tcttcgtgct cgtgtccaac gacgtgcgct cgtgggcggt catcgtgccg tcgttcctgg
    18001 tcggcgccgt catcggctgg atcgtcgcgg tgcgcgtcaa catgatccag atcccggcgc
    18061 tggtcgcgtt ccagcacggc gccggcggtg tcgcagcgtt gcttgtctcc ttcgtcgagc
    18121 tctcccgtgt ctccgccgaa ctcacgccga tcgggaccat ctcgggctac atcgggctca
    18181 tcgtcggctc ggccacgttc gccggcagca tgatcgcagc cgccaagctg gccaacaaga
    18241 tcaagcagca gccgacggtc ttcaagcagc acaacctgat cctcctgctc atcatcgccg
    18301 tgggcgtggc gctcaccgtc ggcagcgccg tcaccggcgg cgccgcgcag caggtgttgc
    18361 tcctcggcct catcgccgcg tcgatgctgc tcggcgtcgt gttcgcgatc cgcatcggcg
    18421 gcgccgatat gcccgtcctg atctcgttcc tcaatgcgac cgccggcctt gccgcggcgt
    18481 tcgtcggcgt ggtcatcgag agcacgctgc tcatcgccgc cggcgcgacg gtggcctcct
    18541 ccggctcgat cctcacgtgg gtgatgtgca ccggcatgaa ccgcagtctc ctcaacgtgc
    18601 tcatcggcca gaagaagacg aagggcggtg cggccaaacc caaggctccc gaggccgtcc
    18661 ccgccgcccc cgcccccgcc gccgcggcgg cgcccaccga tcccaagggc cgcgcccttg
    18721 ccgcgctcga ggcggccgag agcgtgatcg tcgtccccgg ctacggcatg gcccaggcgc
    18781 acgccgcctt cgaggtggtg cgcctcgccg acctgctcga gaaggagggc aagcaggtgc
    18841 gcttcgccgt gcaccccgtg gccgggcgca tgcccggcca catgcacgtg ctgctcgccg
    18901 aagccgaggt cgagtacgag aagctcttcg agatgcagga catcaacgac g
//
LOCUS       DASUMX010000117         2773 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_894212, whole genome shotgun sequence.
ACCESSION   DASUMX010000117 DASUMX010000000
VERSION     DASUMX010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_894212"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..436
                     /locus_tag="VFZ86_05810"
     CDS             146..436
                     /locus_tag="VFZ86_05810"
                     /inference="COORDINATES: protein motif:HMM:NF018058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HEX5641842.1"
                     /translation="MSSTEEGPQVQMQRATRPSSPGYGRLIAPAVIAIVIIILILQNT
                     KEDWRIHFFFWWFSLPAALMLVLLLLVGFLIGLAVSALVRRRRRAQRRGESY"
     gene            complement(430..>2773)
                     /locus_tag="VFZ86_05815"
     CDS             complement(430..>2773)
                     /locus_tag="VFZ86_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEX5641843.1"
                     /translation="LCCFGGAAGQHACLVADALGMTQALLHPLAGVLSAYGMGLADVR
                     ALRERTVEAALEEAARPATAGALAELEQAAVSELERQGITAADVRVERRGLVKYRGTD
                     VALPVPWAAPEEMAAAFLELHHARYGFLIPGRALSLEAVAVEAIGAGGHVEAGREPPH
                     AAPRTRGQAAAPADGRTCAPRELGSVFTGGRHNEAPVYVREDLPPAAVVEGPAVICEP
                     TSTTVVEPGWRAEVLAGGELLLRRTSPPARPSARPEHVSPLLLEVFNSLFMSVAEQMG
                     ATLANTAVSVNIEERLDFSCAVFDAEGGLVANAPHLPVHLGSMGASVRAVLERRRGEL
                     RPGDAWLLNSPYAGGTHLPDLTVITPVFPEAGTAGPPAPLFFVASRAHHADVGGVTPG
                     SMPPGSRTIEEEGVLIEDFHLVADGRLREAEAAALLTAAPHPVRDLQQNLADLRAQVA
                     ANARGAAELGRMVTEFGLPVVTAYMRHVQDNAAEAVRRVLDRFAGTAGAPRRFRQELD
                     DGDAIVVSVTIDRAARRAVVDFTGTSPQRADNFNAPEAVAAAAVLYVFRTLVDADIPL
                     NAGCLEPIDIVIPPGSMLSPAWPAAVAAGNVETSQAVTAALLGALGAAAASQGTMNNL
                     TFGDDRYQYYETLCGGAGAGPGWDGCSAVHTHMTNSRLTDPEVLEWRYPVRVEEFRVR
                     AGSGGEGAHRGGDGAVRRLRFLEPLTAAIVSSSRRVPPYGLAGGLPGACGRNAVERAD
                     GAIEELPGVAETLMEPGDVLVVETPGGGGFGAPPGRDPAQ"
BASE COUNT          347 a         1031 c         1059 g          336 t
ORIGIN      
        1 gggcctcccg cccctcacgc gtgcggcgcg aggccggccg ccgagtctcc gcggccggcg
       61 gagccaccgg cgtaccacgc tcacgacaag ggtagaccgg acctacgacg aaccaagtcg
      121 ggacgacgac ggaggagggt caggcatgag ctcgaccgaa gaggggccgc aggtgcagat
      181 gcagcgagcc acgcggccca gcagccccgg gtacggcagg ctcatcgccc cggcggtgat
      241 cgcgatcgtg atcatcatcc tcatcctcca gaacaccaag gaggactggc ggatccactt
      301 cttcttctgg tggttctcac tgcctgcggc gctgatgctc gtcctgctgc tgctggtggg
      361 gttcctcatc ggcctggcgg tgagcgcgct ggtccggcgg cgccggcggg cgcagcgccg
      421 cggcgagagc tactgagccg ggtcgcggcc gggcggcgcg ccgaagccgc cgccgcccgg
      481 ggtctcgacg accagcacgt cgcccggctc catgagcgtc tccgcgacgc cgggcagttc
      541 ctcgatggcg ccgtccgcac gctcgacggc gttgcggccg caggccccgg ggaggccgcc
      601 ggcgagcccg tagggcggca cacggcggct gctcgagacg atcgccgccg tgagcggctc
      661 caggaagcgc aggcgcctga cggccccgtc gccgccgcgg tgcgcgccct cgccgccgct
      721 gccggcgcgg acgcggaact cctccacgcg caccgggtac cgccactcca gcacctccgg
      781 gtccgtgagg cgcgagttgg tcatgtgcgt gtgcacggcg ctgcagccgt cccagcccgg
      841 accggcgccg gcgccgccgc agagcgtctc gtagtactgg taacggtcgt ctccgaaggt
      901 gaggttgttc atcgtccctt ggctggccgc ggccgcgccg agcgcgccga gcagcgcggc
      961 cgtcaccgcc tgtgacgtct ccacgttgcc ggccgcgacc gccgcgggcc acgccggcga
     1021 cagcatgctc cccggcggga tgacgatgtc gatgggctcg aggcagccgg cgttgagggg
     1081 gatgtcggcg tcgaccagcg tgcggaagac gtagaggacg gccgccgccg ccaccgcctc
     1141 gggcgcgttg aagttgtcgg cgcgctgcgg cgaggtgccg gtgaagtcga cgacggcccg
     1201 gcgggccgcc cggtcgatgg tcacgctcac gacgatcgcg tcgccgtcgt cgagctcctg
     1261 gcggaagcgc cgcggcgccc cggccgtgcc ggcgaacctg tcgagcacgc ggcggacggc
     1321 ctcggccgcg ttgtcctgca cgtggcgcat gtacgcggtg accacgggga ggccgaactc
     1381 ggtgaccatg cgtcccagct cggccgcgcc gcgggcgttg gccgccacct gggcgcggag
     1441 gtcggccagg ttctgctgca ggtcgcgcac cggatggggg gcggccgtga gcagcgccgc
     1501 cgcctccgcc tcgcgcagcc tgccgtccgc caccaggtgg aagtcctcga tcaggacgcc
     1561 ctcctcctcg atcgtgcggc tgcccggcgg catcgacccg ggcgtgacgc cgccgacgtc
     1621 ggcgtggtgg gcgcgcgacg cgacgaagaa gagtggcgcg ggagggcctg ccgtgcccgc
     1681 ctccgggaac accggcgtga tcaccgtcag gtccggcagg tgggtcccgc cggcgtaggg
     1741 cgagttgagc agccaggcgt cgccgggccg gagctcgccg cggcgccgct cgagcaccgc
     1801 ccgcaccgag gcgcccatgg acccgaggtg cacggggagg tgcggcgcgt tggcgacgag
     1861 gccgccctcg gcgtcgaaca cggcgcagct gaagtcgagg cgttcctcga tgttcaccga
     1921 cacggcggtg ttggccagcg tggcgcccat ctgctcggcg acggacatga acaggctgtt
     1981 gaagacctcg agcagcagcg gggagacgtg ctcgggacgg gccgagggac gggccggcgg
     2041 cgaggtcctg cgcagcagca gctcgccgcc ggccagcacc tcggcccgcc agcccggctc
     2101 gaccacggtc gtgctcgtgg gctcgcagat caccgccggc ccctcgacga cggcggccgg
     2161 cggcaggtct tcgcggacgt acaccggcgc ctcgttgtgg cggccgccgg tgaagacgct
     2221 cccgagctcg cgcggcgcgc acgtgcggcc gtccgccggc gcggccgcct gaccacgggt
     2281 tcgcggcgcc gcatgcgggg gctcccgccc ggcctccacg tgcccgccgg cgccgatcgc
     2341 ctcgaccgcg acggcctcca gactgagggc gcgcccaggg atgaggaacc cgtagcgcgc
     2401 gtgatgcagc tccaggaacg ccgccgccat ctcctccggc gccgcccagg gcacgggcag
     2461 cgcgacgtcg gtgccgcggt acttgacgag gccgcggcgc tcgacgcgca cgtccgcggc
     2521 cgtgatgccc tggcgttcga gctcggacac ggccgcctgc tcgagctcgg cgagcgcgcc
     2581 ggccgtcgcc ggccgggctg cctcctccag cgcggcctcg acggtgcgct cgcgcagcgc
     2641 gcgcacgtcg gcgaggccca tgccgtaggc gctgagcact ccggccagcg ggtgcaggag
     2701 cgcctgcgtc atgccgagcg cgtcggcgac caggcaggcg tgctgaccgg cggcgccgcc
     2761 gaagcagcag agc
//
LOCUS       DASUMX010000118         2345 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1469608, whole genome shotgun sequence.
ACCESSION   DASUMX010000118 DASUMX010000000
VERSION     DASUMX010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1469608"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1590
                     /locus_tag="VFZ86_05820"
     CDS             <1..1590
                     /locus_tag="VFZ86_05820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEX5641844.1"
                     /translation="GAADVAPVVLVALEVDDAVLDEARRLGAPLVVSHHPLIFDPLER
                     LSDDTLAGRLALRAAREGVAVIAAHTNLDKARGGMADVIAELLGLEAAAPLAPAAADV
                     LKLVGFVPEDDADLVRKALFASGAGVIGEYEHCSWSVGGQGTFFGREGTDPAAGTSAR
                     DETVHEQRLEVVFPRRLRRRVVGAYVAAHPYEEPAFDVVPLENEIASLGLGRLGALPA
                     PTTLAALAAEVAAVLRLPSVRYAGDGRREVRRVAVLPGSGAEAIARGVAQVADVLITG
                     DVKYHEARAAQAQGLALIDAPHGVTEQEGVLRWAERLRDALGPAATVETFRDPAVAVW
                     RESAVGVGEAAAAAPTRAPAPASEAPALGAESAPEPQDTPAPVAAAAPAQPEPPRPVN
                     RDDDRFRLYTDGGARGNPGPAGIGARLMTAAGDVVEDLADFIGKATNNVAEYQALIAG
                     LEIALDRGVERLDVFLDSELVVRQVNGRYKVKDAGLKPLHQQACLLLSKFHEVDVKHV
                     RREQNAAADALVNQAIDAATT"
     gene            1664..1740
                     /locus_tag="VFZ86_05825"
     tRNA            1664..1740
                     /locus_tag="VFZ86_05825"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1698..1700,aa:Gly,seq:gcc)
     gene            1821..1894
                     /locus_tag="VFZ86_05830"
     tRNA            1821..1894
                     /locus_tag="VFZ86_05830"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1853..1855,aa:Cys,seq:gca)
BASE COUNT          308 a          864 c          840 g          333 t
ORIGIN      
        1 ggcgccgccg acgtggcgcc ggtcgtcctc gtcgcgctcg aggtggacga cgccgtgctc
       61 gatgaggccc gccgtctcgg cgcgccgctc gtcgtcagcc accacccgct gatcttcgac
      121 ccgctcgagc ggctctcgga cgacacgctc gccggccggc tcgcgctgcg cgcggcgcgg
      181 gagggcgtcg ccgtcatcgc cgcgcacacg aacctcgaca aggctcgcgg cgggatggcc
      241 gacgtcatcg ccgagctgct cggtctggag gccgccgcgc cgctggcgcc ggcggcggcc
      301 gacgtgctca agctcgtcgg cttcgtgccg gaggacgacg ccgacctcgt gcgcaaggcg
      361 ctgttcgcgt ccggcgccgg tgtgatcggc gagtacgagc actgctcgtg gtcggtgggc
      421 ggccagggca cgttcttcgg ccgcgagggc acggaccccg cggccgggac gtccgcccgc
      481 gacgagacgg tgcacgaaca gcggctcgag gtggtgttcc cgcggcggct gcgccgccgc
      541 gtcgtcggcg cctacgtcgc cgcgcacccg tacgaggagc cggcgttcga cgtcgtcccg
      601 ctggagaacg agatcgcctc gctgggcctc gggcggctcg gcgcgctgcc ggcaccgacg
      661 acgctcgccg cgctggcagc cgaggtcgcg gcggtgctgc ggctgccgtc ggtgcgctac
      721 gccggcgacg gccgccgtga ggtccgccgc gtcgccgtac tgcccggctc cggcgccgag
      781 gccatcgcgc gcggcgtcgc acaggtcgcc gacgtgctga tcaccggcga cgtcaagtac
      841 cacgaggcgc gcgcggcgca ggcgcagggc ctggccctga tcgacgcgcc gcacggcgtg
      901 acggagcagg agggcgtgct gcgctgggcc gagcggctcc gcgacgcgct cggcccggcg
      961 gcgaccgtgg agaccttccg cgatccggcc gtcgctgtgt ggcgggagtc ggccgtcggc
     1021 gtcggcgagg ctgcggccgc cgccccgacg cgtgcgcccg cgccggcgtc cgaggcgccc
     1081 gcactgggcg ccgagtccgc gccggagccg caggacacac cggcgcctgt cgcggccgct
     1141 gccccggcgc agcccgagcc cccccgcccc gtcaaccgcg acgacgaccg cttccgcctc
     1201 tacacggacg gcggcgcgcg cggcaatccc ggccctgccg ggatcggcgc gcgcctcatg
     1261 accgccgccg gcgatgtcgt cgaggacctc gccgacttca tcggcaaggc gaccaacaac
     1321 gtcgccgagt accaggcgct gatcgccggc ctcgagatcg cgctcgaccg cggtgtcgag
     1381 cgcctcgacg tgttcctcga cagcgagctc gtcgtgcgtc aggtcaacgg acggtacaag
     1441 gtcaaggacg cgggcctgaa gccgttgcac cagcaggcct gcctcctcct cagcaagttc
     1501 cacgaggtcg acgtgaagca cgtccggcgc gagcagaacg cggccgcgga cgccctcgtc
     1561 aaccaggcga tcgacgcggc gactacctag cggccgtgcc cgcccgggcc tcccgggaac
     1621 gaattgcgcc ccggcgcgcc ctctggtagc atcccgcctt gctgcgggtg tagctcagtt
     1681 ggctagagcg tctgcttgcc atgcagaagg tcgagggttc gagtcccttc acccgctcca
     1741 atccccctct cggggacgag gcagaggaca gaggcgatgt gctctgtcgt ctgcctttcg
     1801 tgcttgagag gactgcgcgt ggcggcgtag ccaagtggta aggcagaggt ctgcaaaacc
     1861 tccatcaccg gttcgactcc ggtcgccgcc tccaacaaat tccctgcatc tggtggtttc
     1921 tgctcgtacg tgtgcgcacg gaactgccga atccgtcaag actgcccagg ctttgcccac
     1981 agagcccttc aggatgtctg tgtgcgatcc tcgtaggtac ttccccattg gatccggcag
     2041 atgcgagacc acggagacgg gactcctcca cgtggtgacc cggaagcagg atctgccggc
     2101 accgccgtcc actatctcac cgcgctcgac ttccgtagcg acgccaccct ccttcagtgt
     2161 gttcggcggg atggcggcgc tcaggggacg gacggtaagg gcgccgctca ccggctgagt
     2221 ctcggggagt gggagccggc gcggtgaccg caaggcggct gccgcgccgg cccccgtctc
     2281 tggcccaccg cgccgaatcg tgacatgctt caaggcgagg ctgatggcgc gtggggacgc
     2341 ccggc
//
LOCUS       DASUMX010000119        19905 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_176386, whole genome shotgun sequence.
ACCESSION   DASUMX010000119 DASUMX010000000
VERSION     DASUMX010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 19905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19905
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176386"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(426..2048)
                     /gene="gcvPB"
                     /locus_tag="VFZ86_05835"
     CDS             complement(426..2048)
                     /gene="gcvPB"
                     /locus_tag="VFZ86_05835"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012786246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HEX5641845.1"
                     /translation="MTPEELKSAAEEKVAALAKLRRDGGVLHDYRAPRWDEPFIMEQG
                     TPGERGVFVPLAEEGVTAEVGAAADYIPEGMARAAAPELPEMAQYQVLRHYLRYAQMT
                     LGMDLGSDISEGTCTMKYSPKMHEELIRGHKAADVHPWQDEDTLQGLLEIVYETGQYL
                     KSISGMDEFTLQPGGGSHAVYTNACVMRRYFEARGELDQRSEMITTIFSHPCDAATPM
                     VAGFKVITVMPDENGYPDLEAFRAAVSEKTVGCMITNPEDTGIYNPQIDKYVEILHDA
                     GALAFTDQANANGILGFARARDAGFDACHFNLHKTFSSPHGCEGPASGAYMVTEKLAP
                     YLPVPMVTKLGDRYHLDYDRPESIGRVRGFLGNWMAVIRAYAWIRTMGPEGLKEVAEI
                     SVLNNNYLDNLLMAIPGVDRPYAEGHRRLDQVRYSLEKLKEDTGVGTEALRDRMVDFG
                     IQSYWMSHHPWVVPEPFTPEPCETYSKADLDEWAAVIKKGVDEAYADAQFVLDAPHAQ
                     PIDQMRRLEAMEDPEQWAFTWRKYKRKFKDKV"
     gene            complement(2045..3454)
                     /gene="gcvPA"
                     /locus_tag="VFZ86_05840"
     CDS             complement(2045..3454)
                     /gene="gcvPA"
                     /locus_tag="VFZ86_05840"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HEX5641846.1"
                     /translation="MAEKPFIHPYIPNSVPATKQAMLDELGLGSVEEIYAEIPDRLRF
                     KGTLDIPPAILSERALRKHVQGLLDKNTSSVDFLNFCGAGCWQHHVPAVVDEIINRAE
                     FVSAYCGGDYSDLGKYLTRYEFNSMLGELLDYDAVANPIYDWGDVAGRSFRMARRITG
                     RDTVLVPAHMSPMRLMEARTLCQPEAMPNSTKIRFYEWDRETGMADLEDLKAKLDDSY
                     AAVYWESPGYLGTIEANGAEIVKLAHSVGALAINGVDPITLGVLAPPGALGADIACGD
                     IQPLGMHMHAGGGCSGFIAFKDDDPVFAQECPLELYTVMETATPGQYAVGEAMAERTS
                     YGARDQGKDWVGTASGLWTIAAAVYMALMGPQGFRELGETCIARSHYAAKKLGELPGV
                     EIKLSPSFFKEFVVNFDGAGKTVAEVNKALLGHRILGGKDLSAEFPELGQSALTCVTE
                     YYDQADIDALVSAVKEVTA"
     gene            complement(3510..4298)
                     /locus_tag="VFZ86_05845"
     CDS             complement(3510..4298)
                     /locus_tag="VFZ86_05845"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003236548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5641847.1"
                     /translation="MTSDMSGKTVVVTGAAGGVGAAVARRFAEAGAALVLGDLDAVRL
                     EELCGQIAAYSEVHGVSCDVTSVADIENLMAEAVAATGRIDVLVNTAGLWVEGPSEEM
                     TEADWDRVVDVNLKGTFFACSRAIPELKKTEGCIINLSSDAGVVGTPETAIYTASKGG
                     VSLMTKALAIELAPHLVRVNAVCPADIMTPMLQYQADTYGEGDPEGYFKRLLGSYFQG
                     DKARFITPEEVAELIFFLASAPAAPITGANISIDFGSSAGYGYA"
     gene            complement(4388..5185)
                     /locus_tag="VFZ86_05850"
     CDS             complement(4388..5185)
                     /locus_tag="VFZ86_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019531665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5641848.1"
                     /translation="MARKGRVEGKVALVTGVAKQDSIGFATAKVLAEEGASIAVCDIA
                     EAVHDCAAALRELGATVSSHTADLTKMAEVKAMVAEATECHGRIDVLVNNAGMVVFGQ
                     EEDFSAFQDLTEEAWDFGIAINLKTQFNVTRCVVPGMIERGYGRIVNMSSVTGPVVAN
                     PEEAAYCAAKAGVLGMTRGLALDVAKHGVTVNAVGPGWIATGSQAEGEDVGGLNTPMG
                     RSAFPEEVAKLVCFLGSDDASYITGQLVVVDGGNTIQEYKGPSDLYY"
     gene            5453..10786
                     /locus_tag="VFZ86_05855"
     CDS             5453..10786
                     /locus_tag="VFZ86_05855"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX5641849.1"
                     /translation="MTSVSERPEPVWLRVGEVASALGVSANTVRRWTDVGRIAAHRSP
                     GGHRRYLAEDVRALLPEGAPDGGAQPGDFAELRRQSQTLRSVLQVGLELVALLADDPH
                     DVPAQAARALCELTGAPRCDVYLGDGETVRLVVSAEAGELQTGREGSAWTTREWAPVE
                     GDLAVAEVLCVRASDRGLTRRARLAMQRRGCRSLAWAPMALHGALVGALELSDAGERD
                     FSRHADALQGLARICAEAVAIQRTMDKLARRDKAVRELVDLSHEVAQTHDFERFVLRF
                     AQRLLTAANADCVDVWRAGGGVIRSVVSYTREGEDPGARDTILDTSSYPSLERTLLDH
                     TPLAICDLRDPRLGQTEAELMRGWGFASSLTMPLVAGGELVGLVDLYDDAERDWSADL
                     ASLTSVCQLVAGVFDSTALLDEARETVRLREELVELGADLAAAEEAADVAERAARRLR
                     DATGCEDCDIWWLEEGYLRCLASVDGRGVDETVLGKQLILEHYPSAQQAIADRQILVI
                     SSLDDDRLTDFEREDFSEYSFRSTVSVPLVTNDQVVGMIDLFDVRERDYNEVRGFLPE
                     AARTVADALRNAELLAGLRRGNAALRELVELGDRLNEAGTLEDLARAVAERLRGVLAA
                     EDCDIWQVDDGVLRCLASVDSHGWDADEVGSERELATYEATVAALTRNEPIVVGDLEA
                     TDLSETEMQAYRRWGFRSMVSLPLVVEGRPIGLIDVFDTKVRDYTVHLDLIRNVGRLL
                     AGSFEKAMLVERLEGGNQDLRLLVDSGMEFAATLDVDAVLRTVAERILAVSEADMCDV
                     YRLDGDEFEILVAIGGEYDDDPAGSRYLLHDCSTLLKAAEQRRPVRCTDIMTDPTVTA
                     AELEDARIWGYRESLDVPLTSRGEVIGFISLMNRETRGFPREEVVVGLAQVASQAIAN
                     AGLYRQLDDNLRRMALVSESALELTGSLDLQATLLTTARRLCVGIGVGECEIAVIEGN
                     DLRTLMRVDGGEIDESWMGQRLPLADAAVTREVIATKRPAVVGSLRDPRLTPAVRELN
                     SDHELMSWATLPLIVKDRVIGTVELVESGAERSFTKAELDTAAAICHAAALAIDNAAL
                     FAREKQTARATQLLNDIAARTAASLDLEEVVKAAVDELGQLMTLADYTLLLLEGDTVG
                     RVISSRPQAEGLAGLTVEDFGAGVVASIAAQAVRVLRLPGDLPALEGSPAFAGVAAVT
                     AIALPSDTGLLGVLGLESLDPDAFTSVDRHLLERVGTQLSLAIKNAQLYDEIKQMHLG
                     NLKALSSALNAKDYYTLGHAARVAAYTVMLGRELGWPEDVLAPLEEAAYLHDIGKISI
                     SDRVLLKPGRLNQQEWDQMRQHPTVSADIIRPLFPKDLVLAVRHHHERYDGGGYPDGL
                     SGEDIPALARAMAVVDAYDAMSCRRPYKAALTYAECLAELHRCRGTQFDPAMVDAFLT
                     VLEEVAGRRAKADEIAATAAARIRGETHRALLETDDEQSGAYKEVETILREVRDANPP
                     TRFLTTHAQVDKRYVIGVDPEEDESEKSHLGDEIFVDDELTQVLAGKRPHVNTVFADE
                     FGVWITGLAPIRDVSGDIVAVAVADLPALSPSESEALRGDGRQTFAAMLQSAAVRLSR
                     AEIDAITDALTGLYNHRYLHERLSEELHRARDQQRPLSVLFCDLDHFKRYNDASGHSA
                     GDAVLREVAHVIEQSVRNVDIAARYGGEEFVVLLVETHRETALVVAERIRERIRAAGF
                     TAGNVPLTVSIGVAGYPDDADRREDLLNKADWAMYLAKRRGRDQVAIFGDR"
     gene            complement(10847..12817)
                     /gene="selB"
                     /locus_tag="VFZ86_05860"
     CDS             complement(10847..12817)
                     /gene="selB"
                     /locus_tag="VFZ86_05860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HEX5641850.1"
                     /translation="MRERAGEDPTARPPGRPALTLGTAGHIDHGKTALVTRLTGKNTD
                     RLREERERGISIELGYAELALPSGARLSVVDVPGHERFVRTMVAGATGIDLFLLVIAA
                     DDGVMPQTVEHLAVIELLGVRDGVVALTKADAVDDELLELAAADVGEFLDGTPYAGCD
                     VVTVSARDGRGLDELLAALERVADRAEAGRRAGPARLPVDRVFPLKGIGTVVTGTLWA
                     GEIRPGDVLTVEPGGARAGVRSVQVHDHEAEVVRAGSRAGVNLRGLGRDDVHKGDWLA
                     APELAGLSGRRFDAWVSVLPGARPLRSGDQVRLHHGTAQYLARVAPLEGREIASGSAA
                     AAVVRLDAPALVLPHDRFIIRSLSPVATTAGGEVLLAGAQRWHARERHAAFLVAVRDG
                     DAGAAVAALAGDRGQTGLTRDDLTAAGFGAGEAGAALAAAVAAGELEELPAGPGAAPA
                     ADARWFAAGALAALRAALLAGAAERAAARPERPFSGVAELAALVPALPAAEAGILLDR
                     MAAAGELVAGEGGYAPAGAGVLGDEQELLAAAILARLAAEPMAPPTLATLAEDLRRPA
                     RELGQILEVLARRDEVVRVDKDLWFARAAVEAARRVLLTQLEAAGEVTLAGFRDAAGC
                     GRRNAQALLEYFDREGLTLRRGDVRVARRRRS"
     gene            complement(12825..14273)
                     /gene="selA"
                     /locus_tag="VFZ86_05865"
     CDS             complement(12825..14273)
                     /gene="selA"
                     /locus_tag="VFZ86_05865"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007295030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HEX5641851.1"
                     /translation="MNEAKRTRSAEGEDRQQRLRALAAVDEVLREPGVEELLARYPRA
                     LVVDAVRAVLDRLRAEILEAGVAGEASAAGAADLTPAALVPWIARLLDAAVTPSLRRV
                     INATGVVVHTNLGRALLPRAAVEAVVTAAMSYSDLEYSLARGDRASRQDHIHDVLCTV
                     TGAEDALAVNNNAAAILLALAATARGGEVLVARGQLVEIGDGFRIPDILRESGAELVE
                     VGTTNRTYLRDFEAAWTERTRAVLRVHTSNYRIVGFTTEPALEELATLAHERGAVLID
                     DLGSGALADLELFTEEPALADSVAAGCDIVTFSGDKLLGGPQAGIAVGRAAAIDAMRR
                     HPLARALRIDKLDLAALDVVLRLYLDPARAVAEIPTLAALAQSLDDVRDRAGRLRDLF
                     VGAGVAEDRLELVDTVARAGAGALPVTEVPSVAVAVTVPPDEAGARAELLRRGAPAVV
                     CRVQDGRLLFDLRAVGEDELEDLAGAALIALV"
     gene            complement(14287..14862)
                     /locus_tag="VFZ86_05870"
     CDS             complement(14287..14862)
                     /locus_tag="VFZ86_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641852.1"
                     /translation="MPDDGQKHRPWGEDGRTDPDSLRPGDEEAAAGPGGASVDIVLRA
                     LSVLPFLLVAAIGLIAIVSWVLIIVLGAGSAEPIWDAVAGMSGGELVWQLLLAVLIGA
                     AAVLVTVLGSWATAYGFREDSGRPFWIMTQGVWGLVAVGLVYVERTWDTSLRDLGLSG
                     LEWWFGLGVVAFAMILAGVRLRRAPRYDEDD"
     gene            complement(14873..15946)
                     /gene="selD"
                     /locus_tag="VFZ86_05875"
     CDS             complement(14873..15946)
                     /gene="selD"
                     /locus_tag="VFZ86_05875"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HEX5641853.1"
                     /translation="MGPCDLQAVLDSMPAATGEFARLLETHDDAAVVPVADDLCLVQT
                     VDFFTPIVDTPLWFGRLAAANALSDVYAMGARPLTAMNLVAWPSDLDLGLLGEVLRGG
                     LEKVEEAGAELAGGHSIDDKEPKYGLAVTGVARPDEIVYNGGAEPGDKLVLTKRLGIG
                     VLASGLKNGVISEEAMMPAIEEAAVLNAAAATAMARVGVHACTDVSGFGLAGHLGEML
                     DASGAAAVVHLGALPLHDGVLELIARHVYAGGLRNNREFLLPRLREEGPAGAAEHIAA
                     GGPAAGATPTGAVAGRFAGPVTQQDPLVLSIFDPQTSGGLLLAVAPEKHALLLDALAH
                     AGAGAWTIGEVIAGPTGALVLEG"
     gene            complement(16190..16597)
                     /locus_tag="VFZ86_05880"
     CDS             complement(16190..16597)
                     /locus_tag="VFZ86_05880"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX5641854.1"
                     /translation="MASNIDIVRTGYESFIRGDIQTTLAMFAEDIEWHEPEWAYGPAS
                     GDFCGRDEVLRRIFAPIPELNDSFALEPREFYCDGDTVIVSGMFHVTPKGAEAAVQVP
                     FAHIWTVHGGRIDSLRAYNDVREIVALHSARDD"
     gene            complement(16667..18115)
                     /locus_tag="VFZ86_05885"
     CDS             complement(16667..18115)
                     /locus_tag="VFZ86_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641855.1"
                     /translation="MTAAAVLLIAAALAGAARAGVPARQAGEARTLTTTGPLPPKSVE
                     PAVGARRAVAAAGESVLPVPAYLWRDGCGPTATGMVLGYWDGRGFPDLVPGDASTDTP
                     AAYQMIASHGTAGSPGHYDDYSLPEDDAGGLAPDRSEKPAGDEHPADSVADFMHTSWS
                     AEGLPYGWSFTDMVGPAFAGYVELRLSGVTASYADYYYGSYGSASLTFTVLRQEIDAG
                     RPMVLCVDSTGDGLIDHAVTGIGYRETSGYPEYACWDTWSRSVRWERFRVVSDAYAWG
                     VDSATALSLDDSAAPPPEVDGTAPVTSVSGAGIGWSRTPVTLTFTATDAGSGVDRTEA
                     ALDGQLELSPLAGLPATLDVTGQGTHTVRYRSIDKDGNVETTQACTVRIDGEKPVTSA
                     RAASVRRGARVTLRYRVRDLTPTANVRLVVKTRSGRTRATLRLGRRGTNVLRAARWRC
                     PLARGVYVVAVYATDEAGNRQARPGTARLTVR"
     gene            18312..18755
                     /locus_tag="VFZ86_05890"
     CDS             18312..18755
                     /locus_tag="VFZ86_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004922780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family peroxiredoxin"
                     /protein_id="HEX5641856.1"
                     /translation="MADAPRRADAVWQGSLGQGSGDVSLVTSGAAGPLPVTWASRTER
                     SNGKTSPEELLAAAHASCYCMALSGGLGDAGTPPEKLEVSVTVTFQEVEGGWKTGSSH
                     ITVKGTVPGIDEAIFKKAAEDAKDGCPISGALKGNVELSVEATLA"
     gene            complement(18852..>19905)
                     /locus_tag="VFZ86_05895"
     CDS             complement(18852..>19905)
                     /locus_tag="VFZ86_05895"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX5641857.1"
                     /translation="YLPDGHYAKQGWNMLDRDFSLGEANPLNVVVDGQASDGAVKQSI
                     ARLQQAMEEDGRFAPAQATTNKAGDLTLLTTALAGDPVGESTQALVKHLRDDLIPAAF
                     AGSPAHVYVTGTTAGVVDYLGFFDAWLPVVLLVVLSLSFVLLLIAFRSIVIPVQAILM
                     NLLSVGASYGLLVLVFQKGIGAGLLGLTQVESIEAWVPLMLFALLFGLSMDYQVFLLS
                     RIKERFDETGDTREAVGYGVGRTAGIITGAAAIMVCVFGGMATGELVMFQQMGFGLAV
                     AVLIDATVVRTIIVPAAMELLGERNWYLPRWLRWIPDVSVEGRRHEPAAPKEPRRPRH
                     EPGIAVPRPSLEGAEH"
BASE COUNT         2756 a         7105 c         7256 g         2788 t
ORIGIN      
        1 cccgaacgcc tcgaggacgc ggatgccggg cttcgccggt tgctggggca cgtggtccgc
       61 ctttcgtctg gtcgccgaga ccggccgcga agggcacgtc cggtctcccg ccatgatacc
      121 cgagacgcgc aggaggcggg gggcgcgagc gtgcgccccc gcccccgcgg ccatcgagcg
      181 aaccacccta tgtcctctcg cgactggcgt catggctggc atcatgggtg gcaccttgca
      241 cgacgtcatg agtggcacca tggatggcgc caccggtggt gtcaccgccg atcacgcacg
      301 cccaccgcgg gcccagacgg agtcccggcg gcggcggagc ccggcggggt cccagacgcg
      361 agcggggcgg gggcacccgc ccccgccccg ccctcgcagc cccggacgag atcagctcgg
      421 ggcgttcaga ccttgtcctt gaacttgcgc ttgtacttgc gccaggtgaa ggcccactgc
      481 tccgggtcct ccatggcctc gaggcgccgc atctggtcga tgggctgggc gtgcggcgcg
      541 tcgagcacga actgcgcgtc ggcgtaggcc tcgtcgacgc ccttcttgat caccgcggcc
      601 cactcgtcca ggtcggcctt gctgtaggtc tcgcacggct cgggcgtgaa cggctccggc
      661 accacccacg ggtggtggct catccagtag ctctgaatgc cgaagtcgac catgcggtcg
      721 cggagcgcct cggtgccgac gccggtgtcc tccttgagct tctccaggct gtagcgcacc
      781 tggtcgagcc ggcggtggcc ctcggcgtac ggccggtcga cgccggggat ggccatcagc
      841 aggttgtcga ggtagttgtt gttgagcacc gagatctcgg cgacctcctt gaggccctcg
      901 gggcccatgg tgcggatcca ggcgtaggcg cggatgacgg ccatccagtt gccgaggaac
      961 ccgcgcacgc ggccgatgct ctccggccgg tcgtagtcga ggtggtaccg gtcgcccagc
     1021 ttcgtcacca tcggcaccgg caggtacggc gccagcttct cggtgaccat gtaggcgcca
     1081 ctggccgggc cctcgcagcc gtgcgggctt gagaacgtct tgtgcaggtt gaagtggcag
     1141 gcgtcgaatc cggcgtctcg agcgcgggcg aagccgagga tgccgttggc gttggcctgg
     1201 tcggtgaacg cgagggcgcc ggcgtcgtgc aggatctcga cgtacttgtc gatctgcggg
     1261 ttgtagatgc cggtgtcctc ggggttggtg atcatgcacc ccacggtctt ctcgctgacc
     1321 gccgcgcgga aggcctcgag gtcgggatag ccgttctcgt cgggcatcac ggtgatgacc
     1381 ttgaagccgg ccaccatggg cgtcgccgcg tcgcacgggt gcgagaagat cgtcgtgatc
     1441 atctcgctcc gctggtcgag ctcgccccgg gcctcgaagt acctgcgcat cacgcaggcg
     1501 ttcgtgtaca cggcgtgcga gccgccgccg ggctgcagag tgaactcgtc catgccgctg
     1561 atggacttca ggtactgccc ggtctcgtag acgatctcga gcaggccctg cagggtgtcc
     1621 tcgtcctgcc acgggtgcac gtccgccgcc ttgtggccgc ggatgagctc ctcgtgcatc
     1681 ttgggcgagt acttcatggt gcaggtgccc tcgctgatgt cgctgccgag gtccatgccc
     1741 agcgtcatct gcgcgtaccg caggtagtgg cgcagcacct ggtactgggc catctccggc
     1801 agctcggggg cggcggcgcg ggccatcccc tcggggatgt agtccgccgc cgcgcccacc
     1861 tcggccgtga cgccttcttc ggcgagcggc acgaagacgc cgcgctcgcc cggcgtcccc
     1921 tgttccatga tgaacggctc gtcccagcgc ggcgcgcggt agtcgtgcag cacgccgccg
     1981 tcgcggcgca gcttcgccag cgcggcgacc ttctcttctg cggctgactt cagctcctcc
     2041 ggggtcatgc cgtcacctcc ttgaccgcag acaccaacgc gtcgatgtcc gcctggtcgt
     2101 agtactcggt gacgcaggtg agcgcagact gcccgagctc cgggaactcg gcggagaggt
     2161 ccttgccgcc gaggatccgg tggccgagca gggccttgtt gacctcggcc acggtcttcc
     2221 cggcgccgtc gaagttgacg acgaactcct tgaagaagct cggggagagc ttgatctcca
     2281 ctcccggcag ctcgccgagc ttcttggccg cgtagtgcga gcgggcgatg caggtctcgc
     2341 cgagctccct gaagccctgc ggacccatga gggccatgta cacggcggcg gcgatggtcc
     2401 acagccccga cgccgtgccg acccagtcct tgccctggtc gcgggcgccg tagctggtgc
     2461 gctcggccat ggcctcgccg acggcgtact ggccgggggt ggcggtctcc atcacggtgt
     2521 agagctcgag cgggcactcc tgggcgaaca cggggtcgtc gtccttgaag gcgatgaacc
     2581 cggagcagcc gccgccggcg tgcatgtgca tgcccagcgg ctggatgtcg ccgcaggcga
     2641 tgtcggcgcc gagcgcgccg ggcggcgcga gcacgccgag cgtgatgggg tcgacgccgt
     2701 tgatggcgag tgcgccgacc gagtgcgcca gcttcacgat ctcggcgccg ttggcctcga
     2761 tcgtgccgag atagcccggg ctctcccagt acacggcggc gtacgagtcg tcgagcttgg
     2821 ccttgaggtc ctcgaggtcg gccatgcccg tctcgcggtc ccactcgtag aagcggatct
     2881 tggtgctgtt cggcatcgcc tccggctggc agagcgtgcg cgcctccatg aggcgcatgg
     2941 ggctcatgtg cgcggggacg agcacggtgt cgcggccggt gatgcgccgg gccatgcgga
     3001 acgagcgccc ggccacgtcg ccccagtcgt agatggggtt ggcgacggcg tcgtagtcga
     3061 gcagttcgcc gagcatgctg ttgaactcgt agcgcgtgag gtacttgccg agatcgctgt
     3121 agtcgccgcc gcagtaggcg ctcacgaact cggcgcggtt gatgatctcg tcgacgaccg
     3181 ccggcacgtg atgctgccag cagccggcgc cgcagaagtt caggaagtcg acggacgacg
     3241 tgttcttgtc gagcaggccc tgcacgtgct tgcgcagcgc ccgctcgctg aggatcgccg
     3301 gcgggatgtc cagcgtaccc ttgaagcgca ggcggtcggg gatctcggcg tagatctcct
     3361 ccacgctgcc gagcccgagc tcgtcgagca tcgcctgctt ggtcgcaggc accgagttcg
     3421 ggatgtaggg gtggatgaac ggtttctcag ccatggtcgt tgcggcgctc cttcccttgc
     3481 gcgtgcgtcc gatgcaggtg gtcggcgggt caggcgtagc cgtagcccgc cgagctgccg
     3541 aagtcgatgg agatgttggc gccggtgatc ggcgccgccg gcgcggaggc gaggaagaag
     3601 atcagctccg cgacctcctc cggcgtgatg aagcgcgcct tgtccccctg gaagtagctg
     3661 ccgagcaggc gcttgaagta cccctcgggg tcgccctcgc cgtaggtgtc ggcctggtac
     3721 tgcagcatcg gcgtcatgat gtcggcgggg cacaccgcgt tgacccgcac gaggtgcggc
     3781 gccagctcga tggcgagcgc cttggtcatg aggctgacgc cgcccttcga cgccgtgtag
     3841 atggcggtct cgggcgtgcc cacgaccccc gcgtcggagc tcaggttgat gatgcagccc
     3901 tcggtcttct tgagctccgg gatcgctctg ctgcaggcga agaaggtgcc cttgaggttc
     3961 acgtccacga cgcggtccca gtccgcctcg gtcatctcct ccgagggacc ctccacccag
     4021 aggccggcgg tgttcacgag cacgtcgatg cgccccgtcg ccgccacggc ctcggccatg
     4081 aggttctcga tgtcggcgac gctggtgacg tcgcagctca cgccgtggac ctcgctgtac
     4141 gcggcgatct ggccgcagag ctcctcgagg cgcacggcgt cgaggtcgcc gaggaccagt
     4201 gcggcgccgg cctccgcgaa gcggcgcgcg accgccgcgc cgaccccgcc ggctgctccc
     4261 gtgacgacca ccgtcttgcc gctcatgtcg gacgtcatac gccctccccc gtggcggata
     4321 cggaacggcc gcccgcgaat gctgaacggc cgttcactgt tgcctaagca taccgtcagc
     4381 cggcgcctca gtagtagagg tcgctcggcc ccttgtactc ctggatcgtg ttgccgccgt
     4441 ccacgacgac gagctggccg gtgatgtagc tcgcgtcgtc gctgccgagg aagcacacca
     4501 gcttggcgac ctcctccggg aacgccgagc gccccatcgg cgtgttgagg ccgccgacgt
     4561 cctcgccctc cgcctgggag ccggtggcga tccagccggg gccgacggcg ttgacggtga
     4621 cgccgtgctt ggccacgtcc agcgcgagcc cgcgcgtcat gccgagcacg cccgccttgg
     4681 cggcgcagta tgcggcctcc tcgggattgg cgaccaccgg ccccgtgacg gaactcatgt
     4741 tgacgatgcg gccgtagcca cgctcgatca tccccgggac gacgcagcga gtgacgttga
     4801 actgcgtctt gaggttgatg gcgatgccga agtcccacgc ctcctcggtg aggtcctgga
     4861 aggcggagaa gtcctcctcc tggccgaaga cgaccatgcc ggcattgttg accagcacgt
     4921 cgatcctgcc gtggcactcc gtggcctccg cgaccatggc cttgacctcg gccatcttcg
     4981 tgaggtcggc ggtgtgcgag ctgaccgtcg cgccgagctc gcgcagcgcg gcggcgcagt
     5041 cgtgcaccgc ctcggcgatg tcgcagacgg cgatcgacgc gccttcctcc gcgaggacct
     5101 tcgcggtcgc gaagccgata ctgtcctgct tcgcgacgcc cgtgacgagg gcgaccttgc
     5161 cctccaccct ccctttgcgt gccatcctgt agcgctcctt ggctcggtgt gcactcggcg
     5221 taagctcgcc ggtgacggtc gcccgaatgc tatgccgact gccgggggcg agccaagggc
     5281 ggcggccgcg gcgagccgat ggcgacggct gcggccgcga cgggtgccgg cggacgccgc
     5341 ggcgcagcct ctcgaacctc ccggtcctgc gcgacgacgt gggagctttg gtcaagctcg
     5401 gcgcggacgc tgccgataga cgcgacgaaa tgggcccgcg gaaggggagc aggtgaccag
     5461 cgtcagcgag agacccgagc ccgtctggct gcgcgtcggg gaggtcgcgt ccgcgctggg
     5521 cgtgagcgcg aacacggtgc ggcgctggac agacgtcggg cgcatcgccg cgcaccgcag
     5581 ccccgggggg catcgccgct atctcgccga ggacgtccgc gcgctgctgc cggagggagc
     5641 gcccgatggg ggcgcccaac ccggtgattt cgcggagctc aggcgccaga gccagaccct
     5701 ccgttccgtg ctgcaggtcg gcttggagct cgttgcactg ctcgccgacg acccgcacga
     5761 cgttccggcc caggcggcgc gcgcgctgtg cgaactgacc ggcgcgccgc gttgcgacgt
     5821 gtacctcggg gacggcgaga cggtacggct cgtcgtctcc gcggaagccg gcgagctgca
     5881 gacgggccgc gagggctcgg cgtggacgac ccgggagtgg gcgccggtcg agggcgacct
     5941 cgccgtcgcc gaggtgctct gcgtccgcgc ctcggaccgc ggcctcacgc gacgagcccg
     6001 tctggcgatg cagcggcgcg gctgccgctc gctggcctgg gcgccgatgg cgctgcacgg
     6061 cgctctcgtc ggcgccctgg agttgagcga cgccggcgag cgggacttct cgcgccacgc
     6121 cgacgcgctg cagggcctgg cgcgcatctg cgccgaggcc gtcgcgatcc agcggacgat
     6181 ggacaagttg gcgcgtcgcg acaaagccgt gcgcgagctg gtcgacctct cccatgaggt
     6241 cgcgcagacg catgacttcg agcgcttcgt cctgcgcttc gcgcagcgac tgctcaccgc
     6301 cgcaaacgcg gactgcgtcg acgtctggcg cgccggcggc ggagtcatcc gctcggtcgt
     6361 cagctacacg cgagaaggcg aggacccggg cgcccgcgac acgatcctcg acacctccag
     6421 ctacccctcg ctcgagcgga cgctgctgga ccacacgccc ttggcgatct gcgacctgcg
     6481 tgacccccgg ctcgggcaga ccgaggccga gctcatgcgc ggctggggct tcgccagctc
     6541 gctgacgatg cccctagtcg ccggagggga gctcgtcggg ctggtggacc tgtacgacga
     6601 cgccgagcgt gactggagtg cggacctcgc gtcgctcacc agcgtctgcc agctcgtcgc
     6661 cggcgtgttc gacagcaccg cgctcctcga cgaggcccgt gagacggtcc gcctgcgcga
     6721 ggagctcgtc gagctcggcg ccgacctcgc cgccgcagaa gaggcggccg acgtcgccga
     6781 gcgcgccgcg cgccgcctgc gcgacgccac cggctgcgag gactgcgaca tctggtggct
     6841 cgaggagggg tacctgcgct gcctcgccag cgtggacggc cgaggcgtcg acgagacggt
     6901 gctcggcaag cagttgatcc tcgagcacta cccctctgcg cagcaggcga tcgcggaccg
     6961 ccagatcctc gtcatcagct cgctggacga cgaccgcctc accgacttcg agcgggagga
     7021 cttctccgag tacagcttcc gcagcacggt ctcagtgccc ctcgtgacca acgaccaggt
     7081 ggtcggcatg atcgacctgt tcgacgtgcg tgagcgcgac tacaacgagg tgcggggctt
     7141 cctccccgag gcggcgcgca ccgtggccga cgcgctgcgc aacgccgagc tcctcgccgg
     7201 gctgcggcgc ggcaacgcgg cgctccgcga gctggtcgag ctcggcgacc gcctcaacga
     7261 ggcgggcacc ctggaggacc tcgcccgggc ggtcgccgag cgtctccgcg gcgtcctagc
     7321 cgccgaggac tgcgacatct ggcaggtgga tgacggcgtg ctccgctgcc tcgcgagcgt
     7381 ggacagccac ggctgggacg ccgacgaggt gggatctgag cgcgagctgg ccacgtacga
     7441 ggccaccgtg gccgccctga cgcgcaacga gccgatcgtc gtcggcgacc tcgaggccac
     7501 cgacctgagc gagaccgaga tgcaggcgta ccgccgctgg ggattccgca gcatggtctc
     7561 gctgccgctg gtggtggagg gcaggccgat cggcctcatc gacgtgttcg acaccaaggt
     7621 gcgcgactac accgtgcacc tggacctcat ccgcaacgtc ggccgcctac tcgcgggctc
     7681 gttcgagaag gcgatgctgg tggagcgtct cgagggcggc aaccaggacc tgcgcctcct
     7741 cgtggactcg ggcatggagt tcgccgcgac gctggacgtc gacgccgtgc tccggaccgt
     7801 ggccgagcgc atcctcgcgg tctccgaggc ggacatgtgc gacgtctacc gcctggacgg
     7861 cgacgagttc gagatcctcg tcgccatcgg cggggagtac gacgacgacc ccgccggcag
     7921 ccgctacctg ctgcatgact gcagcacctt gctcaaggcg gcggaacagc ggcggccggt
     7981 gcgctgcacg gacatcatga ccgacccgac ggtgacggcc gcggagctcg aggacgcccg
     8041 gatctggggc taccgcgaga gtcttgacgt gccgctgacc agtcgcggcg aggtgatcgg
     8101 gttcatctcg ctcatgaacc gggagacgcg aggcttcccc cgggaggagg tcgttgtcgg
     8161 cctggcgcag gtcgcgagcc aggcgatcgc caacgcaggg ctctaccggc agctggacga
     8221 caacctgcgg cgcatggcgc tggtgagtga gtcggcgctg gagctgaccg gcagtctcga
     8281 cctccaggcc accctgctca cgactgcccg ccgtctgtgt gtgggcatcg gcgtcggcga
     8341 gtgcgagatc gccgtgatcg agggcaacga cctgcgcacg ctgatgcgcg tggacggcgg
     8401 cgagatcgac gagagctgga tggggcagcg cctgcccctc gccgacgccg cggtgacgcg
     8461 cgaggtgatc gcgacgaagc ggccggcggt cgtcgggtcg ctccgcgacc cgcggctcac
     8521 gccggccgtg cgcgaactca acagcgacca cgagctcatg agctgggcga cgctgccgtt
     8581 gatcgtcaag gaccgcgtga tcggcaccgt ggagctggtg gagagcggcg ccgaacgcag
     8641 cttcacgaag gcggagctcg acaccgccgc ggcgatctgc cacgccgcgg ccctcgccat
     8701 cgacaacgcg gcgctgttcg cccgcgagaa gcagactgcg cgcgcgacgc agttgctcaa
     8761 cgacatcgcg gcgaggacgg ccgccagcct cgacctcgag gaggtcgtca aggcggccgt
     8821 ggacgaactg gggcagctga tgacgctcgc ggactacacc ctgctgctgc tcgaaggaga
     8881 caccgtgggc cgcgtcatct cctcccggcc gcaggcggag gggctggccg gcctcaccgt
     8941 cgaggacttc ggcgccggcg tcgtggcgag catcgcggcg caggcggtgc gcgtgctgcg
     9001 gctgcccggc gacctgccgg cgctcgaggg ttcgccggcc ttcgcgggtg tcgcggcggt
     9061 gacggcgatc gccctgccct ccgacaccgg cctgctcggc gtgctcggcc tcgagagcct
     9121 cgacccggac gccttcacca gcgtggaccg tcacctgctc gagcgcgtcg gcacgcagct
     9181 gtccctggcg atcaagaacg ctcagctcta cgacgagatc aagcagatgc acctgggcaa
     9241 cctcaaggct ctcagctcgg cgctcaacgc aaaggactac tacaccctcg gccacgccgc
     9301 gcgggtggcg gcctacacgg tcatgctggg ccgggagctg ggatggccgg aggacgtcct
     9361 ggcgccgctg gaggaggccg cttacctgca cgacatcggc aagatcagca tctccgacag
     9421 ggtgcttctc aagccgggac ggctgaacca gcaggagtgg gaccagatgc gccagcaccc
     9481 tacggtgagc gccgacatca tccggccgct cttcccgaag gacctcgtgc ttgccgtccg
     9541 ccaccaccac gagcggtacg acggcggagg gtaccccgac ggcctctccg gcgaggacat
     9601 cccggcgctc gcccgcgcca tggcggtggt ggatgcctac gacgccatgt cgtgccggcg
     9661 tccgtacaag gccgcgctca cctacgccga gtgcctggcg gagctgcacc gctgccgcgg
     9721 cacccagttc gaccccgcga tggtggacgc gttcctgacc gtgctcgagg aggtggcggg
     9781 gcgtcgcgcc aaggccgacg agatcgcggc gacggccgcg gcccgcatcc gcggcgagac
     9841 ccacagggcc ctcctggaga ccgacgacga gcagtccggc gcctacaagg aggtcgagac
     9901 gatcctgcgc gaggtacgcg acgcgaaccc gcccacgcgg ttcctgacca cccacgccca
     9961 ggtcgacaag cggtacgtga tcggcgtgga ccccgaggag gacgagagcg agaagtccca
    10021 tctgggcgac gagatcttcg tcgacgacga gctgacccag gtgctggccg gcaagcgccc
    10081 gcacgtcaat accgtgttcg ccgacgagtt cggcgtgtgg atcaccgggc tggcgccgat
    10141 ccgcgacgtc tcgggcgaca tcgtggccgt cgccgtcgcg gacctgccgg cgctctcgcc
    10201 gagcgagagc gaggcgttgc gcggcgatgg ccggcagacc ttcgcggcga tgctgcagtc
    10261 ggcggccgtc cgcctcagcc gcgcggagat cgacgccatc accgacgcgc tcaccggtct
    10321 ctacaaccac cgctacctgc acgagcggct gagtgaggag cttcatcgcg cgcgcgacca
    10381 gcagcggccc ctgagcgtgc tgttctgcga cctcgatcac ttcaagcggt acaacgacgc
    10441 cagcggtcac agcgccggcg acgcggtgct gcgcgaggtc gcgcatgtga tcgagcagtc
    10501 ggtgcgcaac gtcgacatcg ccgcgcggta cggcggcgag gagttcgtgg tgctcctggt
    10561 ggagacgcac cgcgagacgg cgctggtggt cgccgagcgc atccgcgagc gcatccgcgc
    10621 cgccgggttc accgccggca acgttccgct cacggtgagc atcggggtgg ccggctaccc
    10681 cgacgacgcc gaccggcgcg aggacctgct gaacaaggcc gactgggcga tgtacctcgc
    10741 caaacgccgc ggccgcgacc aggtggcgat cttcggcgac aggtgagcgg tcaccgtccg
    10801 ggtcgatccg gccgaggcac gggcgggccc tcgagggcgg ttcgcctcag gagcggcggc
    10861 ggcgcgcgac gcggacgtcg ccgcgccgca gggtcaggcc ctcccggtcg aagtactcga
    10921 ggagggcctg ggcgttgcgg cggccgcagc ccgcggcgtc gcggaagccg gccagcgtga
    10981 cctcgccggc tgcctccagc tgggtcagga gcacgcggcg cgcggcctcg acggcggcgc
    11041 gcgcgaacca caggtccttg tccacgcgca cgacctcgtc ccggcgcgcg agcacctcca
    11101 ggatctggcc gagctcacgc gccggccggc gcaggtcctc ggcgagggtg gccagcgtag
    11161 gcggagccat tggctccgcg gcgaggcgcg ccaggatcgc cgcggccagg agctcctgct
    11221 cgtcgccgag gacgccggcg cccgcggggg cgtagccgcc ttcgccggcg accagctcgc
    11281 ccgccgcggc catgcggtcc agcaggatgc cggcctccgc ggccggcagg gccggcacga
    11341 gcgcggccag ctccgccact ccgctgaacg gccgttccgg ccgcgcggcg gcgcgctcgg
    11401 ccgcgccggc cagaagggcc gcgcgcagcg cggccagggc gccggcggcg aaccagcggg
    11461 cgtccgcggc cggagccgcg cccgggcccg ccggcagctc ctcgagctct ccggcggcca
    11521 cggccgccgc cagcgcggcg ccggcctcgc cggcgccgaa cccggcggcc gtcaggtcgt
    11581 ccctggtcag gccggtctgt ccccggtccc cggcgagcgc cgcgacggcg gcgccggcgt
    11641 cgccgtcgcg caccgcgacg aggaacgccg cgtgccgctc gcgcgcgtgc cagcgctgcg
    11701 cacccgccag gaggacctcg ccgccggccg tggtggccac cggcgagagc gagcggatga
    11761 tgaagcggtc gtgcggcagc acgagcgccg gcgcgtccag ccgcacgacc gcagccgccg
    11821 ccgaccctga ggcgatctcc cggccctcga gcggtgcgac ccgcgccagg tactgcgccg
    11881 tgccgtggtg cagccgcacc tggtcgccgg agcgcagcgg ccgcgcgccg ggcagcaccg
    11941 agacccaggc gtcgaagcgg cgcccggaca gccctgcaag ctcgggcgcc gccagccagt
    12001 cgcccttgtg gacgtcgtcg cggccgaggc cgcgcaggtt cacgccggcg cggctgccgg
    12061 cccgcaccac ctcggcctcg tgatcgtgca cctggacgct gcgcacgccc gcgcgcgcgc
    12121 cgcccggctc caccgtcagc acgtccccgg ggcggatctc gccggcccac agcgtgcccg
    12181 tgaccaccgt gccgatgccc ttgagcggga acacgcggtc caccggcagg cgggccgggc
    12241 cggcgcgccg gcccgcctcg gcgcggtccg cgacgcgctc gagcgccgcg agcaactcgt
    12301 ccaggccgcg gccgtcgcgg gcgctcacgg tgacgacgtc gcaaccggcg tacggcgtgc
    12361 cgtccaggaa ctcgcccacg tcggcggcgg cgagctccag cagctcgtcg tccacggcgt
    12421 ccgccttggt cagcgcgacg acgccgtccc gcacgcccag cagctcgatg acggcgaggt
    12481 gctccaccgt ctgcggcatc acgccgtcgt cggcggcgat gacgagcagg aagaggtcga
    12541 tgccggtggc tcccgccacc atcgtgcgca cgaagcgctc gtgcccgggc acgtcgacca
    12601 cgctcagccg cgctccggag ggcagcgcca gctccgcgta accgagctcg atggagatgc
    12661 cccgctcgcg ctcctcgcgc aggcggtcgg tgttcttgcc ggtcagacgg gtgaccagcg
    12721 ccgtcttgcc gtgatcgatg tgaccggccg tgccgagggt gagcgcggga cggccgggag
    12781 gccgagcagt cggatcttcc ccagctcgtt cgcgcaccgt ggcctcagac gagggcgatg
    12841 agggccgcgc cggcgaggtc ctcgagctcg tcctcgccga ccgcacgcag atcgaagagc
    12901 agacggccgt cctgcacgcg gcagacgacg gccggcgcgc cgcggcgcag cagctcggcg
    12961 cgcgccccgg cctcgtccgg gggcaccgtg accgcgaccg cgacgctcgg cacttcggtc
    13021 accggcagtg ccccggcgcc ggcgcgggcg acggtgtcga cgagctcgag gcgatcctcg
    13081 gccacgccgg cgccgacgaa caggtcgcgc aggcggccgg cgcggtcgcg cacgtcgtcg
    13141 agcgactggg cgagcgcggc cagcgtcggg atctcggcga ccgcgcgcgc cgggtccagg
    13201 tagagccgca ggacgacgtc gagcgcggcg agatcgagct tgtcgatgcg cagcgcgcgc
    13261 gcgagcgggt ggcggcgcat cgcgtcgatc gcggcggccc ggccgacggc gatgccggcc
    13321 tgggggccgc cgagcagctt gtcgccgctg aaggtgacga tgtcgcagcc ggcggcgacg
    13381 ctgtccgcca gcgccggctc ctccgtgaac agctcgaggt cggcgagggc gccgctgccg
    13441 agatcgtcga tgagcaccgc gccacgctcg tgcgcgaggg tcgccagctc ctccagcgca
    13501 ggctccgtcg tgaacccgac gatgcggtag ttgctggtgt gcacgcgcag taccgcccgc
    13561 gtcctttcgg tccaggcggc ttcgaagtcc ctgaggtagg tgcggttcgt cgtccccacc
    13621 tcgaccagct ccgcgccgct ctcgcgcagg atgtcgggga tgcggaagcc gtcaccgatc
    13681 tcgacgagct ggccgcgggc gacgagcacc tcgcccccgc gcgccgtggc cgcgagcgcg
    13741 agcaggatgg ccgcggcgtt gttgttgacg gcgagcgcgt cctcggcgcc ggtgacggtg
    13801 cagagcacat cgtggatgtg gtcctggcgc gaggcgcggt cgccgcgcgc caggctgtac
    13861 tcgaggtccg agtagctcat ggcggcggtg acgacggcct cgaccgccgc ccgcggcagc
    13921 agcgcgcgcc cgaggttggt gtgcaccacg accccggtcg cgttgatgac gcggcgcagc
    13981 gacggcgtga ccgccgcgtc cagcagccgc gcgatccagg ggacgagggc ggccggcgtg
    14041 aggtccgccg ccccggcggc ggacgcctcg ccggccaccc ccgcctccag gatctccgcc
    14101 cgcaacctgt ccagcaccgc gcgcaccgcg tcgaccacca gcgcacgcgg gtaccgcgcg
    14161 agcagctcct cgacgccggg ctcgcgcagc acctcgtcca cggccgcgag cgcacgcagc
    14221 cgctgctggc ggtcctctcc ctccgcgctc ctcgtgcgct tggcctcgtt cacgcgccct
    14281 ccgcgctcag tcgtcctcgt cgtaccgcgg cgcgcgccgc aggcgcactc ccgcgaggat
    14341 catcgcgaac gctaccacgc ccagcccgaa ccaccactcg aggcccgaca ggccgaggtc
    14401 ccggagcgac gtgtcccacg tgcgctccac gtacacgagt cccacggcga ccagtcccca
    14461 caccccctgg gtcatgatcc agaacggccg gcccgagtcc tcccggaacc catacgccgt
    14521 ggcccacgag ccgagcacgg tgacgagcac cgccgcggcg ccgatcagga cggcgagaag
    14581 aagctgccag acgagctcgc cgccggacat ccccgcgacc gcgtcccaga tgggctccgc
    14641 cgagcccgcc ccgagcacga tgatgagcac ccagctgacg atcgcgatga ggccgatggc
    14701 ggcaacgagc aggaagggga ggacggaaag ggcccgcagg acgatgtcga ccgacgcgcc
    14761 ccccggcccg gccgcggcct cttcgtcgcc gggcctcagc gaatccggat cggtcctgcc
    14821 gtcctcaccc cacgggcgat gcttctgacc gtcgtccggc accggaacgg ccctaaccct
    14881 cgagcacgag tgcgccggtg gggccggcga tgacctcacc gatggtccag gcgccggcgc
    14941 cggcatgcgc gagcgcgtcg aggaggagcg cgtgcttctc gggcgcgacg gccagcagca
    15001 agccacccga ggtctgcggg tcgaagatgc tgaggacgag cgggtcctgc tgcgtgaccg
    15061 ggccggcgaa gcggccggcg accgcgccgg tcggagtggc gccggccgca ggcccgccgg
    15121 ccgcgatgtg ctcggcggcg cccgcgggtc cctcctcgcg caggcgcggc agcaggaact
    15181 cccggttgtt gcgaaggccg ccggcgtaga cgtgccgggc gatgagctcg agcacgccgt
    15241 cgtgcagcgg gagcgcgccg aggtggacca cggccgcggc gccgctcgcg tcgagcatct
    15301 ccccgaggtg tccggcgagg ccgaacccgc tcacgtcggt gcaggcgtgc acgccgacgc
    15361 gggccatggc cgtggccgcc gcggcgttga ggacggcggc ctcctcgatg gccggcatca
    15421 tggcttcttc gctgatgacg ccgttcttga gcccgctcgc gaggacgccg atgccgagcc
    15481 gcttggtcag cacgagcttg tcgcccggct cggccccacc gttgtagacg atctcgtccg
    15541 gcctggcgac gccggtgacg gccaggccgt acttgggctc cttgtcgtcg atggagtggc
    15601 cgccggcgag ctcggcgccg gcctcctcga ccttctccag gccgccgcgc aggacttcgc
    15661 cgaggaggcc caggtccaga tccgacggcc aggccaccag gttcatggcc gtcagcggac
    15721 gggcgcccat cgcgtagacg tcgctcaggg cgttggccgc cgccaggcgg ccgaaccaga
    15781 gcggcgtgtc gacgatgggc gtgaagaagt cgaccgtctg caccaggcag aggtcgtcgg
    15841 cgaccggcac cacggcggcg tcgtcgtgcg tctccaggag gcgggcgaac tcgccggtgg
    15901 cggcgggcat gctatcgagg acggcctgca ggtcgcacgg acccagcttg gccgctcagc
    15961 cgctcttggc cgcgaactgg gtgaggcgga tcttctcggt cataccgcaa gcttacccca
    16021 gccgccgcgg gcgccggcga ccccggggat cagtcaggcg acccggagac tgcatcgtga
    16081 tctcttcgaa gatatccggg cacagcgtgc agcacggatc ctccctggcc cccggcgggg
    16141 gaacggcggc ggcggggaat ggcggcgctc ggtcggtcgc accgcctggt cagtcgtcgc
    16201 gggcgctgtg cagcgcgacg atctcgcgca cgtcgttgta ggcgcggaga ctgtcgatgc
    16261 ggccgccgtg caccgtccag atgtgcgcga acggcacctg gaccgcggcc tccgcaccct
    16321 tcggcgtcac gtggaacatg ccgctgacga tgaccgtgtc gccgtcgcag tagaactcac
    16381 gtggctcgag ggcgaaggag tcgttgagct cggggatggg cgcgaagatc ctgcggagca
    16441 cctcgtcgcg gccgcagaag tcgcccgagg ccggcccgta ggcccactcg ggctcgtgcc
    16501 actcgatgtc ctccgcgaac atggcgagcg tggtctggat gtcgccacgg atgaagctct
    16561 cgtagccggt gcggacgatg tcgatgttgc tggccatgtc acgagggttc cccgacgcag
    16621 gatggtcaca ccgtccggac gacgccgggc gcgccggagc cgggcgtcag cggaccgtga
    16681 ggcgggccgt gccgggacgg gcctgccggt tgccggcctc gtcggtggcg tagacggcga
    16741 ccacgtagac gccgcgcgcg agggggcagc gccagcgcgc ggcgcgcagg acgttggtgc
    16801 cgcgccggcc gagccgcagg gtggcgcgcg tccggccgct gcgcgtcttg acgacgaggc
    16861 ggacgttcgc ggtcggcgtc aggtcgcgga cgcggtagcg cagcgtgacg cgcgcgcccc
    16921 ggcggacgct cgccgcgcgc gcgctcgtca ccggcttctc gccgtcgatc ctgaccgtac
    16981 aggcctgcgt cgtctcgacg ttgccgtcct tgtcgatgga gcggtagcgc accgtgtgcg
    17041 tgccctggcc ggtcacgtcc agtgtggccg gcagcccggc gagcggcgac aactcgagtt
    17101 gcccgtcgag cgccgcctcc gtgcggtcga cgcccgagcc cgcatcggtg gccgtgaacg
    17161 tcagggtgac cggcgtgcgg ctccagccga tgccggcgcc gctgacgctg gtgaccggcg
    17221 ccgtcccgtc cacctccggc ggcggggcgg ccgagtcgtc cagcgagagc gcggtcgcgc
    17281 tgtccacgcc ccaggcgtag gcgtcggaca cgacgcggaa ccgctcccag cggacggacc
    17341 tggaccacgt gtcccaacag gcgtactcgg ggtagccgct ggtctcgcgg tagccgatgc
    17401 cggtgacggc gtggtcgatg aggccgtcgc cggtgctgtc gacgcagagc accatcggcc
    17461 tgccggcgtc gatctcctgc ctcagcacgg tgaaggtcag cgaggctgac ccgtacgacc
    17521 cgtagtagta gtcggcgtag gaggcggtca cgccggacag gcgcagctcc acgtaccccg
    17581 cgaacgccgg cccgaccatg tcggtgaagc tccagccgta ggggaggcct tcggcgctcc
    17641 aggaggtgtg catgaagtcg gcgacggagt cggcggggtg ctcgtcgccg gccggcttct
    17701 cgctcctgtc gggcgcgagc ccgccggcgt cgtcctcggg gagggagtag tcgtcgtagt
    17761 ggcccggaga gccggccgtg ccgtgcgagg cgatcatctg gtaggccgcc ggggtgtcgg
    17821 tgctcgcgtc acccgggacg aggtcgggga agccgcgtcc gtcccagtag ccgaggacca
    17881 tgcccgtcgc cgtcggcccg cagccgtcgc gccacaggta ggcggggacg gggaggacgc
    17941 tctcgccggc ggcggcgacg gcgcgacggg cgccgacggc cggctccacc gacttcggcg
    18001 gcagcggccc ggtcgtcgtg agggtccgcg cctcgcccgc ctgacgtgcc gggacgccgg
    18061 cgcgtgcggc gccggcgagg gcggcggcga tcagcaggac tgccgccgcc gtcatggcgg
    18121 cgcgcccggc gagggctcgc cgtgatcgtg acggacgtga gctcacggtc cctgcatcgg
    18181 ccgctcgcgg gcaaagcctt agcctgcgcc ggttcgattt ccgcctccgg cggggatgtc
    18241 attggcgtcg gtggacgcca cgcccccacg cggcgtcccc caccaacggt cccgcccaag
    18301 gaggttccat catggccgat gcaccacgcc gcgccgacgc tgtctggcag ggctcgctcg
    18361 gacagggctc cggagacgtc tcgctcgtga ccagcggagc ggcaggtccg ctccccgtca
    18421 cctgggcgtc tcgcaccgag cgctcgaacg gcaagaccag cccggaggaa ctcctcgcgg
    18481 ccgcgcacgc gagctgctat tgcatggcgt tgtcgggcgg cctcggcgat gcgggcacgc
    18541 cgccggagaa gctcgaggtc agcgtaacgg tgacgttcca ggaggtcgaa ggcgggtgga
    18601 agaccggctc ctcgcacatc acggtcaaag gcacggtgcc cggtatcgac gaggcgatct
    18661 tcaagaaggc ggcagaggac gccaaggacg gctgccccat cagcggcgcg ctcaagggca
    18721 acgtggagct gagcgtggag gcgacgctgg cctgagcctc accgcggtcg cgccggccgg
    18781 acggccgccg cagaggacga cgaaagggca gggcgcccgc aggcgccctg ccctttctgc
    18841 tctcctctga gctagtgctc ggcgccttcg agagaaggcc gcgggacggc gatgccgggc
    18901 tcgtgccggg ggcggcgggg ctccttcggc gccgccggct cgtgccggcg cccctccacg
    18961 ctgacgtcgg ggatccaccg cagccagcgc ggcaggtacc agttgcgttc gccgagcagc
    19021 tccatcgccg ccggcacgat gatcgtccgg accacggtgg cgtcgatcag caccgcgacc
    19081 gcgagcccga agcccatctg ctggaacatc acgagttcgc ccgtggccat gccgccgaac
    19141 acgcagacca tgatcgcggc cgcgccggtg atgatgccgg cggtgcggcc gacgccgtag
    19201 cccacggcct ctcgcgtgtc gccggtctca tcgaagcgct ccttgatgcg gctgagcagg
    19261 aacacctggt agtccatcga gaggccgaag agcagggcga agagcatgag cggcacccag
    19321 gcctcgatgc tctccacctg cgtgaggccc aggagaccgg cgccgatgcc cttctggaac
    19381 accagcacca gcagcccgta ggaggcgccg acgctcagga ggttcatgag gatcgcctgc
    19441 accgggatga cgatcgagcg gaaggctatc agcaggagca cgaagctcag gctgaggacg
    19501 accagcagca ccaccggcag ccaggcgtcg aagaagccga ggtagtcgac gacgcccgcc
    19561 gtcgtgccgg tcacgtacac gtgggcgggg ctgccggcga aggcagcggg gatcaggtcg
    19621 tcgcgcaggt gcttcaccag cgcctgcgtg gactcgccca cggggtcgcc ggccagggcg
    19681 gtggtgagca gcgtcaggtc gccggccttg ttggtggtgg cctgggcggg tgcgaagcgg
    19741 ccgtcctcct ccatcgcctg ctggagcctg gcgatgctct gcttgacggc gccgtcgctc
    19801 gcctggccgt ccaccacgac attcagcggg ttggcctcgc cgagcgagaa gtcgcggtcg
    19861 agcatgttcc agccctgctt ggcgtagtgc ccgtcgggca ggtag
//
LOCUS       DASUMX010000120        25082 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1038223, whole genome shotgun sequence.
ACCESSION   DASUMX010000120 DASUMX010000000
VERSION     DASUMX010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 25082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25082
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1038223"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="VFZ86_05900"
     CDS             <1..464
                     /locus_tag="VFZ86_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641858.1"
                     /translation="AADPLTAPGTETTPGAAVTPPPEEAPQTAAQSLAEQLDRFAVVV
                     LAKARQATGVLQRFAVLKIFKGDAPQVVELEVDDRPADAGRLHLLMLDPTTSGDEAFR
                     SPEPIPSLTRAEGDIAFGRALAVTYTYHGEPTMVREYAAGTDPDLVSLPIP"
     gene            498..1154
                     /locus_tag="VFZ86_05905"
     CDS             498..1154
                     /locus_tag="VFZ86_05905"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5641859.1"
                     /translation="MDVPSRYFRDAKPAFLQLIDPRGLRVLDLGCGGGHNGAMLKKAG
                     AREVVGVERDPGAAQEARKRLDRVVECDLGELDPAELGGRPFDAILASDVLEHLADAE
                     DVLARAVTLLRPGGAVVLSLPNVANVYVFSQLLLKTWPRRGSGIFDRTHVRWFAKRDM
                     VRLLQGAGLEVLRVEPYFTRYRAIRATSLVLSLYVFRDYWARQFLLLAVKPDRPGEGR
                     "
     gene            1151..1918
                     /locus_tag="VFZ86_05910"
     CDS             1151..1918
                     /locus_tag="VFZ86_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006447389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HEX5641860.1"
                     /translation="MTDLLEGKSFLVTGAANPRSIAWAVAKAVKRHGGAVALTYQAER
                     LKRRVGQLAEELDGAPMIELDVGSDAAVRRAVDELQEQMPVLDGMVHSIAWAPAEDLQ
                     GSFVQTSRDGFAQANDISSYSLIATAREARRLMTRGGAIVTMTYAGSWRVVPNYNVMG
                     PAKASLESAVRYLAADLGPEGIRVNAVSAGPIATVAARTIKDFSGMLELLESHTPLRR
                     NVTSDEVADATVFLLSDLSRAITGEVVHVDAGAHATI"
     gene            complement(1986..3008)
                     /locus_tag="VFZ86_05915"
     CDS             complement(1986..3008)
                     /locus_tag="VFZ86_05915"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEX5641861.1"
                     /translation="MAHVVARPFDLRTAAPFGISRGTHREFERFVIEMRQGDLVGLGE
                     SAPNARFGEERQRDMDLLAELAPEIADLEGPAAVEAYCEALAAQGVPALRAGLSAAAW
                     DLAGKQAGEPVWRMLGLARPSVRTSYTIALGAPEEMLAQARAASAFGTLKVKLGFAGD
                     LEVARRLKLALPETTFRYDVNEGWDRERAAAALAVLEDLDAELVEQPLPATDTEGMAW
                     LKERTRLPLLADEAVLTLDDLDAVGDACDGVVIKLAKAGGLAAAFALISACTQLGLRV
                     MLGCMVESSLGIAAGLQLAGLADFVDLDGALLLGQDPFEGIEAEGDLLTASDEPGLGI
                     RPAARA"
     gene            3134..4147
                     /locus_tag="VFZ86_05920"
     CDS             3134..4147
                     /locus_tag="VFZ86_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5641862.1"
                     /translation="MGAVENTLLRLTDLTVDFASQEGTVHAVDRVSFEVAPGELVGLV
                     GESGCGKSVTASAIIGLTGMLPNATVSGGIGFLGRDLLTLPEAEVRAIRGRDISMIFQ
                     DPVTSLNPVLSIERLMTEGLEVHLGMSHAEAVRRSIELLGEVGIPQAAGRIKDYPHQF
                     SGGMRQRVMIAIALSCNPRLLLADEPTTALDVTIQAQILRLLKKLSREHEAAVIVITH
                     DLGVVAGMCRRILVMYAGRIVESGPAKPLFAHPHHPYTVGLLRSVPRLGEPRRDTLQT
                     IAGLPPDLANLPPGCAFAPRCDLQTDACWASVPELEPTDYGRLSACLHSDLLRETAAE
                     TTA"
     gene            4195..5235
                     /locus_tag="VFZ86_05925"
     CDS             4195..5235
                     /locus_tag="VFZ86_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015189558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEX5641863.1"
                     /translation="MVRCEDLRVHFPITSGVVLQRKVGAVRAVDGLTFDIRSGETLGL
                     VGESGCGKSTTGRAILQLIRPTTGRVLYEGGDLAELKGGRLREQRKRMQMIFQDPFAS
                     LNSRMTIGDIIGEPLLVHRMGGKAERKAKVRELLETVGINPNTLNRYPHEFSGGQRQR
                     VGIARALAVEPNFIVCDEPISALDVSIRAQIINLLQHLQREFGLTFLFIAHDLSVVRH
                     ICDRVAVMYLGRIVELAENAALYGEPLHPYTQALLSAVPIPDPEAEERRRPVVLSGDV
                     PSPVEPPSGCHFHTRCPAARNGLCDVDDPPLLEVRPGRWAACHLITATDYPHIRAGED
                     DLAVVVEGGGAA"
     gene            5232..6257
                     /locus_tag="VFZ86_05930"
     CDS             5232..6257
                     /locus_tag="VFZ86_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015314629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5641864.1"
                     /translation="MTYVPPEGPGHDRRIQGLEPEIEVDLGLSHPSGASEIGTAVGAA
                     GMWSPDTLLVEHRSLWGEIWRRFRRDKFAMIGVVVIVLIVLIAVLAPWIAPHDPTTQY
                     DEGITMDGMPVGSSAQFPLGTDTLGRDLLSRLMYGARVSLVVGVCANGFALVLGVIFG
                     LTAGYFRGWIETLLMRVTDVMMAFPIILLAVALVSVLKPSIWILILVIGIVYWTPMTR
                     VIHGEVLSIREKEFVDAARLTGCSSARILSRHILPHLVAVILVYTSLGLATTILFEAT
                     LSYIGLGVQPPTPSWGQMISEGQSYYLSAPHLVIYPGLAIMITVLAFNLVGDGLRDAF
                     DPQQRRR"
     gene            6298..7980
                     /locus_tag="VFZ86_05935"
     CDS             6298..7980
                     /locus_tag="VFZ86_05935"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5641865.1"
                     /translation="MTRPSIKGGAVATAVLGLVLALALGLAACGADTSDEGGSSPTAS
                     TDQPKQGGTLTLSYLSEPSSLDPAVAWNVIDWQIEHDIYQGFLQYAHESGQAGTELIP
                     CLATEVPSVENGGISADGTVYTFHLREGVNFQPPVSREVTAADFKYSFERMMREPRAP
                     ATSFYMGVVGADEFMDGKADEITGFKAVDDYTVEITLESPDLSFLNAVTMDFCDVVPK
                     EWVEKWGKQFNRNPLGTGPFVFQEWTPGREIVLTRNPDYWEEGKPYLDGVNYQLSFNP
                     PTALLKLERGEVDVLGDGIPVADLPRVNADPKWKEQVFSQPLVAISYMFMNVEMKPFD
                     DVKVRQALSWAIDRDKLVKLQAGQAMSLWQFYPEGMPGYEEGKVYYGYDPAKAKQLLA
                     EAGYPDGFETMLYTDNVDPNPKLWQSVQADLAAVGVQAGLKTMSNNTYYVQQGTPNTL
                     TAGSFGWWMDFPDPSDWIAPLFSKASAVEGGMNSSFWWSPELETMFADAQKMTDPEAR
                     IAKFTEMQAYIAEQAPYVPCYQPIQTTMCSETTGGFYLHPVYQIDPTQYWKK"
     gene            8022..8951
                     /locus_tag="VFZ86_05940"
     CDS             8022..8951
                     /locus_tag="VFZ86_05940"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5641866.1"
                     /translation="MGKYVVRRLLWMVVVLFFVSLITFLLAFAVPGDPAKSIAGTKAT
                     AEVLQRIRQDLGLDEPLWKQYGLYLWRLLHGNMDYSYETQLPVATAILQRFPATVMVA
                     MFGIFFELLIGIPIGMTSALRQYGFRDRAFTVFSLLFLSLPAFFLGMLLLYIFAFQWP
                     IFPLGGYEGWTAPIYGILPGLTIGLGGAAWYSRMLRSSMLDILGADYVKAARAKGLPE
                     RTVVWRHIMRNAWSPIVTLLGMDVGWFLGGVLVIEIVFGIPGIGGQAYAAIQTQDLPM
                     IMGTVLFAALLVTISNFVVDLAYTWLDPRVKYS"
     gene            9025..10695
                     /locus_tag="VFZ86_05945"
     CDS             9025..10695
                     /locus_tag="VFZ86_05945"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HEX5641867.1"
                     /translation="MRSRLLAVAEYLRARPVQDFVAGAALLIIVLFPFAYIDRREQLI
                     GVPAAIATLIIIGGALGGGPRVGAGLAFVGGVAYDVLVITDRWLIAGPTTIVVVILWL
                     AAGFGVGLLGDRYRGQVARALDQAHDSRDALDRVVGATPLFHARGGPAAVARAICDVA
                     LETFDCQLAALFAIEGERLRLLARSPYLARTAERLIMEPSFELQQELAENLLPQFVHD
                     VREPFGPRMPRAVTSDPDQVSAIRAPVLLDDQPVALLALSWSRRMPEPERGDLGVVQR
                     FAEHAAVALAQAQRAQAQQDVAALYRRFQSSLSPVVKVESDHIKVATYYRPGEARMLL
                     GGDFIDAVTRPNGSVAAVIGDVTGHGPDAAALGASLRAAWRALALRGASLQANMKTLN
                     SLTLDESDRAEEGESGLGLLATVCAVEIDGAGRRARFVNAGHPMPLLLGAEVVSLEHT
                     PGMMLGVEQYGDWAPHEVTLPEDWGLLLYSDGIVEARLGPGTRSRLGLDGLRAALADL
                     WLRRAITAADLERLVEGVQADRQHVLEDDVTLLLLSHDGQRHAHGHVI"
     gene            complement(10750..11487)
                     /locus_tag="VFZ86_05950"
     CDS             complement(10750..11487)
                     /locus_tag="VFZ86_05950"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5641868.1"
                     /translation="MARAERDASVGRPVAAAPAASNGHVASSRQAPSGGDPRQGMSPP
                     ALNILAAAQRVLVEKGFSGLTLRAIAQESGENSAMVQYYFGNKEGLVEAMIDSVFRDD
                     QQDAAAAMSTVTSEDRLPRFVDGLRTISLSRSFRVFFDILPYALRNESFRSRMARAYA
                     SYRQIKRDWLRHEGEPAAACPEHPDEEALLGVAELMTAVVDGLAIQEAIDDDFDMRRA
                     YAVLEFMLQRALPDLLESGVPEAAVAD"
     gene            11585..13123
                     /locus_tag="VFZ86_05955"
     CDS             11585..13123
                     /locus_tag="VFZ86_05955"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEX5641869.1"
                     /translation="MAETKTEVKTLGYMVDGEWRESKSDTWVEITNSNTGEVIARTPM
                     CTEAEVTEAIESANRAYPAWASMPIQKRTEILFTWRPKLIERMDEIATLVSTELGKNL
                     DEARGEVIKIIEAIDIAVGAPMLMKGESLMNVSTGHDTVSYREPLGVFAGIAPFNFPA
                     MIPFGWMLPICIACGNTFVLKSANFVPLTSHLLLDMLYEAGLPKGVVNMVTAEKEEAE
                     VLLKHPAIRGVSFVGSTPVGKHVYSIAAAHGKRVQAQTQAKNHGLVLADASLERTARG
                     IINSTFGCAGMRCMALPVCVVEESIADEFVALMKKFALERSVGDATLPATELGPVVSK
                     AHQQSVLRWIETAIEEGAELVLDGRNVTDGLPDELKGGHYVGPTIFDHVTEDMLCGRE
                     ECFGPILFVKRVKDFEEGIELMNNNPYANGSCIFTESGYHSREFARRTHAGMVGINVG
                     IPVPVSFFPFAGHKDSFFGDNHVFGRDGFQFFTETKCVTTRWFTEADKKQKQVSTWEG
                     TVNR"
     gene            13320..13769
                     /locus_tag="VFZ86_05960"
     CDS             13320..13769
                     /locus_tag="VFZ86_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641870.1"
                     /translation="MAHAFPEITAYGPALRFALEAEQACADFAAAAGVLAPTQEWQDK
                     LEEVVCTHDDRVQKLMAKQGQLGDKASAPAGTLKREPYLGTLDAEPAISWPAVVVQLA
                     QAEEDSARYHEEFAMQCADTLELQARLFGKSAQQNRAYAAELRELLG"
     gene            complement(14165..15550)
                     /locus_tag="VFZ86_05965"
     CDS             complement(14165..15550)
                     /locus_tag="VFZ86_05965"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5641871.1"
                     /translation="MATPPDHPAVPEPSPERETRHAPLVALKNVDFAYDERPVLRRIS
                     FTLEAGDFVGIVGPNGSGKSTLVDLIDGIHQPVAGEVLVKGLATRRYKRKAMAREVAL
                     VPQHFDLDFDLAVREVVEMGAYCRGKDPAACGDPELMLARLGVAELASRRFSELSGGE
                     KQLAVLAQALMQQAPLLLLDEPASALDVSHQLRLFDLLKELNAGGLTVLCILHDLNLA
                     LHYFDKLLVLSDGEVAAFGPPDEVLAPEVVEAVYGVRAYLHRHAGRTYLTFSPRLRGE
                     RRGRVHLVCGGGTGAGMMRELVDAGYEVTAGVLNALDTDEETGSELGLHMAVEAPFSP
                     VGEEAHAENLQLMRAADLVVVTAVPVSHGNARNLEAARDALAAGIPVWADEGVRASDF
                     VGAVAGLAEGGAQFFAGEEALLAAVRGRSLPGGRAPVVSPAAARVAAPAAADDPTPTA
                     SGDPPPGEAAT"
     gene            complement(15550..16548)
                     /locus_tag="VFZ86_05970"
     CDS             complement(15550..16548)
                     /locus_tag="VFZ86_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006523039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX5641872.1"
                     /translation="MSKLARTGTWPGILLVSGMLAVCLVLALALGPAPLSVGRIWQLL
                     FAGPVPGDLDNAIFWQIRMPRVLTAALVGAALAVSGVILQDLFLNPLTDPYVTGVSSG
                     AALGATIGIVLHLSNLSWITVLAIVGGLGTLAIVWMAARRRGRIDVFVLLLAGVTIAY
                     LASAVVTVLMIKANQDMTAIIYWLMGSFSGRGWPDVRIALVTIPFMVLPLFFTREMDI
                     MLQGEKRALELGVEVERTKRMLLVTAGVLTALAVSVSGIIGFVGLVVPHIVRLLVGPG
                     HRGLLPVSLLAGASMMVVADLLSRTVIAPNEIPVGVVTTFVGAPLFVYLLRRGRRA"
     gene            complement(16545..17507)
                     /locus_tag="VFZ86_05975"
     CDS             complement(16545..17507)
                     /locus_tag="VFZ86_05975"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HEX5641873.1"
                     /translation="MTTIRFGRLTAALLLVAVLTLGAAALSACGSGDDTAASSSPSAA
                     AAAGPITVTDDSGVEVTLEQPAEAIVSLAPANTEIAYAVGAGDKMVAGTSYDDYPEEA
                     TALPKIGDFANPNVEKIASFAPDLVLAAGGIQADLRGKLEDLGMTVYVVDPKTYDGVI
                     ATIENVGKLAGTEDGAAAVTAEMTAARQEVQDAVGDLPAATTFLEIYGKPLMTAGADT
                     FIDDMITIAGGENIGATAGSGFPNFSTEVLVQTDPQVYIADSGSMSEPGEIDARAGFG
                     ELTAVKDGKVYIIDDSIIARPGPRLAEGLRALAGYIHPEAETAQ"
     gene            complement(17504..18328)
                     /locus_tag="VFZ86_05980"
     CDS             complement(17504..18328)
                     /locus_tag="VFZ86_05980"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HEX5641874.1"
                     /translation="MASDAPRTDVPSANGRSAGASLAASRFFCSWSGGKDAYLALQRA
                     VERGGAAAALVCMLHEDGGVSRGHGLPLALLEEQAAALGVPLVTRATTWDGYESTFVA
                     LLHELRAQGIEAGVFGDIDLQAHRDWVEGVCEVAGLGCHLPLWQEPRRRLLAEFLAGD
                     SRATIVAVDSSKLSPEFLGLVLDDAVIARLEAAGADACGEEGEYHTMVTAAPLFSRPV
                     PLVWDGVEERDGHHLLRDMRLGATVTPAAPPAGASPQLVDIVRSRLVKDRRESPPS"
     gene            complement(18318..18770)
                     /locus_tag="VFZ86_05985"
     CDS             complement(18318..18770)
                     /locus_tag="VFZ86_05985"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEX5641875.1"
                     /translation="MTDPKPAHEIEAVFRQKGIPFTRQRRQIWHFFAGADRAVTITEA
                     ADALRSDGVGQATVYRSVQLLSDLGLLARVQDGRGEICYIAPPIGHSHPLICGVCRTV
                     VRFDGDGDVAELEARLAAATGFSIYGHHLEVYGICPSCRSAREGEHGE"
     gene            19014..19355
                     /locus_tag="VFZ86_05990"
     CDS             19014..19355
                     /locus_tag="VFZ86_05990"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HEX5641876.1"
                     /translation="MICPECGAEYVPGFTRCADCLVDLVEPGSQDDAPEPARGEALAE
                     PVCVYRATQRGRLPLAESVLRSAGIPFVASGEHLQQLTGLDVFGPAELYVAACDVDDA
                     RAVLAGLDGEQ"
     gene            complement(19380..20012)
                     /locus_tag="VFZ86_05995"
     CDS             complement(19380..20012)
                     /locus_tag="VFZ86_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX5641877.1"
                     /translation="MRQVLCYGDSNTWGADPADDARRFEWPVRWPGVLQRELGGGYHV
                     IEEGLGGRTTVYDDPLLPRRSGRQLLAPALESHAPLDLVVLMLATNDISYAHISAAEA
                     ADGAGELAHLVQRCELGPAGAAPRLLLVCPPPVGPFENNWRPGLWVGADEKSRALPRE
                     FARVASALQVPWLDAGKLITTSALDGWHLEASQHELLGVAVAAKVREILA"
     gene            complement(20043..20429)
                     /locus_tag="VFZ86_06000"
     CDS             complement(20043..20429)
                     /locus_tag="VFZ86_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008445545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5641878.1"
                     /translation="MARVVHFEIHATEPEQLIAFYSGLLGWTFTPTQGTEYWLIGTGP
                     ADEPGIDGGLVRRPIPGPADAPVLNAFPCTVAVDSLDATLAKGDELGAVVALPRMAVT
                     GVGWLAYVKDPDGNLLGLLESDPKAA"
     gene            complement(20461..20928)
                     /locus_tag="VFZ86_06005"
     CDS             complement(20461..20928)
                     /locus_tag="VFZ86_06005"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5641879.1"
                     /translation="MRRAGRADVPELVALMRAFYAEAGFTLDEERATAGFASLLADAR
                     LGRVWLIEPAAGYIVVTFVHAMEFGGMTAVVDDFYVRPEARGEGLGTAALAAARRACE
                     DLGMRAMRVEVGVDNTAAQAVYRSAGFQPLPGHGLMAATLAPSCSARKLEHGG"
     gene            complement(20958..21296)
                     /locus_tag="VFZ86_06010"
     CDS             complement(20958..21296)
                     /locus_tag="VFZ86_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5641880.1"
                     /translation="MGRTAVFLKASDDGYLDALPGIRRRTLSHGAATLMAEFRLQAGY
                     PLPVHEHPHEQTGYLVSGRLLLTIGPETHDVGPGDSWSIPGGVPHGAEVLEDAVAVEV
                     FSPLREDFLS"
     gene            complement(21262..22047)
                     /locus_tag="VFZ86_06015"
     CDS             complement(21262..22047)
                     /locus_tag="VFZ86_06015"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5641881.1"
                     /translation="MAQKSAAERLFWMLGVERTDDVLDLGCGTGHLAQEIRVLTDGRV
                     VGIDLSPEMIALARQAAPATIEFVTGDVHALDMPEAFDVILSNSAFQWFKDGELALRN
                     CLAALRPGGRMAMQAPARQEYCPQFVHAVARLAGDPRTAETWSHFRTPWTFYETGEEY
                     GRLFEAAGFSVVAAEMEELREHATPERALEMFESGAAAGYLNPECYDAPLPAGYAQAA
                     RELILDDLSSRAGGDGLVELLFSRVYVLARKPDGQDRGVPQGQ"
     gene            complement(22150..23028)
                     /locus_tag="VFZ86_06020"
     CDS             complement(22150..23028)
                     /locus_tag="VFZ86_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009024189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HEX5641882.1"
                     /translation="MKKPDRNALVALPVVLAIAALVALAGSQGGSTVGSLPVFALCVI
                     VAFAIQWVAFVPAYVLQSERFFDLTGGLTYITVAVVAVLFSDAPDARSYLLLAMVVIW
                     AVRLASYLFLRIRRAGADTRFDAIKPSFPRFLNTWTLQGLWVTLTLAAALAAITTTER
                     VGIGTVTAIGLLVWVAGFGVEVVADAQKSRFRADPANKGRFIHTGLWSWSRHPNYFGE
                     ILLWVGVAIVALPVLRGWQWITLISPVFVALLLLRVSGVPLLEKSADKKWGGQLDYEA
                     YKHRTSLLVPRPPRRG"
     gene            complement(23025..23618)
                     /locus_tag="VFZ86_06025"
     CDS             complement(23025..23618)
                     /locus_tag="VFZ86_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020126892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5641883.1"
                     /translation="MTAAGGGHSRRADVALRLWAAGDLWLLQRLLGDPEMTRHLGGPE
                     SQEAIRARHERYLAADPEKHGLFAVTAGPAAEAVGWVGYWESEWHGELVWECGWNVLP
                     EAQGRGVATAAATLMIDDARRRGRHRHLHAIQTVGNAASNALCRRLGFELLGEVEVDY
                     PSGVVMRSNDWLLDLEKTAPSPGARASTRGRQDRMTR"
     gene            complement(23615..24229)
                     /locus_tag="VFZ86_06030"
     CDS             complement(23615..24229)
                     /locus_tag="VFZ86_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEX5641884.1"
                     /translation="MKYLSDLFARNRAWAAERTGADPEFFARLAHLQRPDLLWIGCSD
                     SRVPANEIVGLAPGELFVHRNVANVVPDTDLNAMAVIEYAISSLGVRHVIVCGHYRCG
                     GVRAALEGHQAGAVALWLQPLRNLAGEHRDELDALPSDAARWDRLCELNVAAQVLAVA
                     RSEAVQSAWERGADLAVHGWIYDLEDGLLRDLEVTLGAGAAGGW"
     gene            24305..>25082
                     /locus_tag="VFZ86_06035"
     CDS             24305..>25082
                     /locus_tag="VFZ86_06035"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5641885.1"
                     /translation="MHETRLLIIGAGVAGLTAGCYAARSGYRTTILEMGQTPGGLCTS
                     WRRKGYLFDGSVAGLAGTSPEAPIHRLWRDIGVADYCPLYDPVDFGTVVAPDGRAVTV
                     STDIDRLEAHLRENFPRDAAAIGEFARALRACLHLDIPFRAETGLAGIVAGAHTGVAS
                     LRSLPALVKYGRLTLRRFNARLRDPFCRQVFDNLVHFGGQDVPMLTVLLPIAYAHRRM
                     TGIPRDGWLAFGRAIERRFLELGGEIRYGAKVEHLETGGGA"
BASE COUNT         3805 a         8935 c         8806 g         3536 t
ORIGIN      
        1 cggctgccga cccgttgacc gcgccgggaa cggagacgac cccgggagcg gcggtgaccc
       61 cgcccccgga ggaggccccg cagacggcag ctcagagcct cgccgagcag ctcgaccgct
      121 tcgccgtcgt ggtgctcgcc aaggctcgtc aggcgaccgg agtgttgcag cgcttcgccg
      181 tcctcaagat cttcaagggt gacgctccgc aagtggtcga actcgaggtc gacgatcggc
      241 cggccgatgc gggcaggctt catctgctca tgctcgaccc cacgacgtcc ggcgacgagg
      301 cgttccgctc tccggagccc atcccctcgc tcacgagggc ggagggtgac atcgccttcg
      361 gccgggcgct cgccgtgacg tacacgtacc acggagagcc cacgatggtg cgcgagtacg
      421 ccgccggcac cgacccggac ctggtcagcc tgccgatccc ctgatccgcc gcgggacgct
      481 cgcggtatga tgcggcgatg gacgtgccct cccgctactt ccgcgacgcc aagcccgcct
      541 tcctgcagct gatcgacccg cgcggcctcc gcgtgctcga cctcggctgc ggtggcggcc
      601 acaacggcgc gatgctcaag aaggccggcg cgcgcgaggt cgtaggggtc gagcgcgacc
      661 ccggcgcggc gcaggaggcg cgcaagcgcc tcgaccgggt cgtcgagtgc gacctcggcg
      721 agctggaccc ggcggaactg ggcggccggc cgttcgacgc catcctcgcc agcgacgtgc
      781 tcgagcacct cgcggacgcg gaggacgtgc tcgcccgggc ggtcacgctg ctgcgccccg
      841 gcggcgccgt cgtgctctcg ctgcccaacg tcgccaacgt gtacgtcttc tcgcagctcc
      901 tgctcaagac gtggccgcgg cgcggctccg gcatcttcga ccgcacgcac gtgcgctggt
      961 tcgcgaaacg cgacatggtc cggctgctgc agggcgcagg gctcgaggtg ctccgcgtgg
     1021 agccctactt cacccgctac cgcgcgatca gggcgacgtc gctggtcctg tcgctgtacg
     1081 tcttccgcga ctactgggcg aggcagttcc tgctgctcgc cgtgaagccc gaccgacccg
     1141 gggagggacg atgacggacc tgctggaggg caagagcttc ctcgtcacgg gcgccgccaa
     1201 cccgcgctcg atcgcctggg cggtggccaa ggcggtcaag cgccacggcg gcgccgtcgc
     1261 gctcacgtac caggcggagc ggctcaagcg gcgcgtcggg cagctcgcgg aggagctcga
     1321 cggcgcgccg atgatcgagc tcgacgtcgg cagcgacgcg gccgtgaggc gcgccgtgga
     1381 cgagctccag gagcagatgc cggtgctcga cggcatggtg cacagcatcg cctgggcgcc
     1441 ggcggaggac ctgcagggca gcttcgtcca gaccagccgc gacgggttcg cgcaggccaa
     1501 cgacatcagc tcgtactcgc tcatcgccac cgcgcgcgag gcgcggcgcc tgatgacgag
     1561 gggcggcgcg atcgtcacca tgacgtacgc cggttcgtgg cgggtcgtgc ccaactacaa
     1621 cgtcatgggg cccgccaagg cgtcgctgga gagcgccgtg cgctatctcg cggcggacct
     1681 ggggccggag gggatccgcg tgaacgcggt gtccgcaggc cccatcgcca ccgtagcggc
     1741 gcgcacgatc aaggacttca gcggcatgct cgagctcctg gagagccaca cgccgctgcg
     1801 ccgcaacgtc accagcgacg aggtcgcgga cgcgacggtg ttcctgctca gcgacctcag
     1861 ccgcgccatc accggcgagg tcgtgcacgt ggacgccggg gcgcacgcga cgatctgacc
     1921 cgagacgctc ccggcgcggt ctgtccgcca ctcgcggtcg tggggccgcc tccacgcggc
     1981 gccggtcagg ctcgagcggc cggccggatg cccaggccgg gctcgtcgga cgcggtcagc
     2041 aggtcgccct ccgcctcgat cccctcgaac ggatcctggc ccaggagcag cgctccgtcg
     2101 aggtccacga agtcggccag gccggccagc tgcaggccgg cggcgatgcc caggctggac
     2161 tcgaccatgc agccgagcat cacgcgaagc ccgagctgcg tacacgcgga gatgagggcg
     2221 aaggcggcgg cgaggcctcc cgccttcgcc agcttgatca ccacgccgtc gcaggcgtcg
     2281 cccacggcgt cgaggtcgtc cagggtcagc accgcctcgt cggcgagcag cggcaggcgc
     2341 gtgcgctcct tgagccacgc cataccttcg gtgtcggtgg cgggcagcgg ctgctcgacg
     2401 agctcggcgt cgaggtcctc gaggaccgcg agagcggcgg cggctcgctc gcggtcccat
     2461 ccctcgttga cgtcgtagcg gaacgtcgtc tccggcagcg cgagcttgag ccggcgcgcc
     2521 acctcgaggt cgccggcgaa gccgagtttg accttgagcg tgccgaacgc ggaggcggcg
     2581 cgcgcctgcg ccagcatctc ctcaggcgcg ccgagggcga tcgtgtacga cgttcgcacg
     2641 gagggacgag cgagccccag catgcgccac acgggctcgc ccgcctgctt gccggcgagg
     2701 tcccaggcgg cggcgctcag gcccgcccgc agggccggca caccctgcgc cgccagcgcc
     2761 tcgcaatacg cctcgacggc ggccggcccc tcgaggtcgg cgatctccgg cgcgagctcg
     2821 gcgaggaggt ccatgtcccg ctgccgctcc tcgccgaagc gcgcgttcgg agcactctcg
     2881 cccaggccga caaggtcccc ctgccgcatc tcgatcacga agcgctcgaa ctcgcggtgg
     2941 gtcccgcggg agatgccgaa cggagcggcc gtgcgcagat cgaagggccg tgcgacgacg
     3001 tgtgccatga ggtcatccct tcccggagac gcagaggacg gccctcatgc ttaccagccc
     3061 gccgggcctg ggtcaaagga cggcgccagg cggcggccct cacgtccgag ggcgtgcggt
     3121 agactgacga ggcatgggtg ctgtggaaaa cacgcttctg cgtctcaccg atctgaccgt
     3181 cgacttcgcg tcgcaggagg gcacggtcca cgcggtggac cgggtgagct tcgaggtggc
     3241 tcccggcgaa ctcgtcgggc tcgtcggcga gagcggctgc ggcaagagcg tcaccgcgag
     3301 cgccatcatc ggcctcaccg gcatgctccc caacgccacc gtgagcggcg gcatcgggtt
     3361 cctgggcagg gacctgctca cgctcccgga ggccgaggtg cgcgccatcc gcggccgcga
     3421 catcagcatg atcttccagg accccgtcac cagcctgaac ccggtgctca gcatcgagcg
     3481 gctgatgacc gagggtctcg aggtgcacct gggcatgagc cacgccgagg cggtgcgtcg
     3541 ctccatcgag ttgctgggcg aggtgggcat cccccaggcc gcgggccgca tcaaggacta
     3601 cccgcaccag ttcagcggcg gcatgcgcca gcgcgtcatg atcgccatcg cgctcagctg
     3661 caaccccagg ctcttgctcg ccgacgagcc cacgaccgcg ctggacgtga ccatccaggc
     3721 gcagatcctg cgcctgctca agaagctctc gcgcgagcac gaggcggccg tcatcgtcat
     3781 cacccacgac ctcggcgtgg tggcgggcat gtgccggcgc atcctcgtca tgtacgccgg
     3841 gcgcatcgtc gagagcggcc cggcaaagcc gctgttcgcg cacccgcacc acccctacac
     3901 cgtcggcctg ctgaggagcg tgccccggct gggcgagccg cgacgcgaca cgctgcagac
     3961 gatcgcagga ctgccgccgg acctcgccaa cctgccgccc ggttgcgcgt tcgcgccgcg
     4021 ctgcgatctc cagaccgacg cctgctgggc gtccgtgccc gagctggagc ccactgacta
     4081 cggccgcctc tcggcgtgcc tccacagcga cctgctgcgc gagacggcgg cggagacgac
     4141 ggcgtgaaca gcgccgcggc caccgcgggc gccacggccg ccgaaggctc cacgctggtg
     4201 cgctgcgagg acctgcgcgt ccacttcccc atcaccagcg gggtggtgct gcagcggaag
     4261 gtcggcgccg tgcgcgccgt ggacggcctc accttcgaca tcaggagcgg agagacgctc
     4321 ggcctcgtcg gcgagagcgg ctgcggcaag agcaccaccg ggcgcgccat cctgcagctc
     4381 atccgcccca ccaccggacg cgtgctgtac gagggaggcg acctcgccga gctcaagggc
     4441 ggcaggctgc gcgagcagcg caagcgcatg cagatgatct tccaggaccc gttcgcgagc
     4501 ctgaacagcc gcatgaccat cggcgacatc atcggcgagc ccctgctggt ccacaggatg
     4561 ggcggcaagg cggagcgcaa ggcgaaggtg cgcgagctcc tggagacggt gggcatcaac
     4621 ccgaacacgc tgaaccgcta cccgcacgag ttcagcggcg gccagcgcca gcgcgtcggc
     4681 atcgcccggg cgctggccgt ggagcccaac ttcatcgtct gcgacgagcc catcagcgcc
     4741 ctcgacgtgt cgatcagggc gcagatcatc aatctgctgc agcacctgca gagggagttc
     4801 gggctcacct tcctgttcat cgcgcacgac ctctcggtgg tgcggcacat ctgcgaccgg
     4861 gtggccgtga tgtacctcgg gcgcatcgtc gagctcgccg agaacgcagc gctctacggc
     4921 gagcctctgc acccgtacac gcaggcgctc ctgagcgcgg tgccgatccc cgacccggag
     4981 gccgaggagc ggcgccggcc ggtggtgctc tccggcgacg tgcccagccc ggtggagccg
     5041 cccagcggtt gtcacttcca cacgcgctgc ccggcggccc ggaacggcct ctgcgacgtg
     5101 gacgacccgc cgctcctgga ggtgcgcccc ggccgctggg ccgcgtgtca cctcatcacg
     5161 gcgaccgact acccgcacat ccgcgccggc gaggacgacc tcgccgtcgt ggtcgaaggc
     5221 ggtggcgctg cgtgacctac gtgccgccgg agggacccgg tcacgaccgc aggatccagg
     5281 gcctcgagcc cgagatcgag gtcgacctcg ggttgagcca cccgtcgggc gcgtccgaga
     5341 tcggcacggc cgtcggcgcc gccggcatgt ggagccccga caccctgctg gtcgagcacc
     5401 gctcgctgtg gggcgagatc tggcggcgct tccgccgcga caagttcgcg atgatcggtg
     5461 tggtcgtcat cgtcctgatc gtcctgatcg ccgtcctggc tccctggatc gcgccgcacg
     5521 accccaccac gcagtacgac gagggcatca ccatggacgg catgcccgtc gggtcgtcgg
     5581 cgcagttccc gctcggcacc gacaccctcg gccgcgacct gctcagccgg ctcatgtacg
     5641 gcgcccgcgt cagcctcgtc gtcggcgtct gcgccaacgg cttcgcgctc gtcctcggcg
     5701 tgatcttcgg cctcaccgcc ggctacttcc gcggctggat cgagaccctg ctcatgcgcg
     5761 tcacggacgt gatgatggcg ttccccatca tcctgctcgc cgtggcgctg gtgagcgtcc
     5821 tcaaaccgag catctggatc ctgatcctcg tcatcggcat cgtgtactgg acgccgatga
     5881 cgcgggtgat ccacggcgag gtgctcagca tccgcgagaa ggagttcgtc gacgcggcgc
     5941 ggctcaccgg ctgcagcagc gcgcgcatcc tctcgcggca catcctgccg cacctcgtcg
     6001 ccgtcatcct cgtctacacc agcctgggcc tcgccaccac gatcctcttc gaggcgacgc
     6061 tcagctacat cggcctcggc gtacagccgc cgacgccgtc ctggggccag atgatcagcg
     6121 agggccagag ctactatctc tcggcgccgc acctcgtcat ctatcccggc ctcgccatca
     6181 tgatcaccgt gctcgcgttc aacctcgtgg gggacgggct acgcgacgcg ttcgacccgc
     6241 agcagcgaag aagatgacca caccacgtac cgtatgggga cgtccaagga ggaaggtatg
     6301 actcgtccgt ctatcaaggg aggcgccgtc gcgacggcgg tgctggggct cgtcctggcg
     6361 ctcgcgctgg ggctcgccgc ctgtggcgcc gacaccagcg acgagggagg cagcagcccc
     6421 accgcgtcga cagaccagcc caagcagggc ggcacgctca cgctctcgta tctctccgag
     6481 ccctcgagcc tggacccggc agtggcctgg aacgtcatcg actggcagat cgagcacgac
     6541 atctaccagg ggttcctgca gtacgcacac gagtccggcc aggccggcac cgagctcatc
     6601 ccctgcctgg ccacggaagt accgagcgtg gagaacggcg gcatctcggc cgacggcacc
     6661 gtgtacacgt tccacctgcg agagggcgtc aacttccagc cgccggtcag ccgcgaggtc
     6721 acggcggccg acttcaagta cagcttcgag cgcatgatgc gcgagccgcg cgccccggcc
     6781 acatcgttct acatgggcgt cgtcggcgcc gacgagttca tggacggcaa ggccgacgag
     6841 atcaccgggt tcaaagcggt cgacgactac accgtcgaga tcacgctcga gagccccgac
     6901 ctctcgttcc tcaacgccgt caccatggac ttctgcgacg tggtgcccaa agagtgggtc
     6961 gagaagtggg gcaagcagtt caaccgcaac ccgctcggca ccggcccgtt cgtgttccag
     7021 gagtggacgc ccggccgcga gatcgtgctc acccgcaatc ccgactactg ggaagagggc
     7081 aagccgtacc tcgacggcgt caactaccag ctctcgttca acccgccgac ggcgctgctc
     7141 aagctcgagc gcggcgaggt ggacgtgctc ggcgacggca tcccggtcgc cgacctgccg
     7201 cgggtcaacg ccgaccccaa gtggaaggag caggtcttct ctcagccact ggtggcgatc
     7261 agctacatgt tcatgaacgt cgagatgaag ccgttcgacg acgtcaaggt gcgccaggcg
     7321 ctcagctggg cgatcgaccg cgacaagctg gtcaagctcc aggcgggtca ggcgatgagc
     7381 ctctggcagt tctatcccga gggcatgccg ggctacgagg agggcaaggt ctactacggg
     7441 tacgaccccg ccaaggccaa gcagctgctc gcggaggccg gctatcccga cggcttcgag
     7501 acgatgctct acaccgacaa cgtcgacccg aacccgaagc tgtggcagtc ggtgcaggcg
     7561 gacctcgccg ccgtcggcgt gcaggccggc ctcaagacca tgagcaacaa cacctactac
     7621 gtgcagcagg gcacgcccaa cacgctcacg gccggcagct tcggctggtg gatggacttc
     7681 cccgacccga gcgactggat cgccccgctg ttcagcaagg cgagcgcggt ggagggcggc
     7741 atgaactcca gcttctggtg gagccccgag ctcgagacga tgttcgccga tgcgcagaag
     7801 atgaccgacc ccgaggcgcg catcgccaag ttcacggaga tgcaggccta catcgcggag
     7861 caggcgccct acgtgccctg ctaccagccc atccagacga cgatgtgctc cgagaccacg
     7921 ggcggcttct acctgcaccc cgtgtatcag atcgacccca cccagtactg gaagaagtag
     7981 cacgatgacc gtggcgcgac gcgaggcggg caggagcggc gatgggtaag tacgtcgtcc
     8041 gccggctgct ctggatggtg gtggtgctct tcttcgtgag cctcatcacg ttcctgctcg
     8101 ccttcgccgt gcccggcgac cccgccaaga gcatcgccgg caccaaggcg accgccgagg
     8161 tcctccagag gatccggcag gatctcggcc tggacgagcc tctgtggaag cagtacggcc
     8221 tgtacctgtg gcggctgctg cacggcaaca tggactacag ctacgagacc cagctgcccg
     8281 tggcgacggc gatcctgcag cgctttcccg ccaccgtgat ggtggccatg ttcggcatct
     8341 tcttcgaact gctcatcggc atccccatcg ggatgaccag cgccctgaga cagtacggct
     8401 tccgcgaccg cgcgttcacc gtgttcagcc tgctgttcct gagcttgccg gcgttcttcc
     8461 tcggcatgct cctcctgtac atcttcgcct tccagtggcc gatcttcccg ctgggcggct
     8521 acgagggatg gacggcgccg atctacggca tcctgcccgg gctcacgatc ggcttgggcg
     8581 gcgccgcctg gtactcgagg atgctgcgca gcagcatgct cgacatcctg ggcgccgact
     8641 acgtgaaggc cgctcgtgcc aaagggctcc ccgagcgcac ggtggtctgg cggcacatca
     8701 tgcgcaacgc ctggagcccg atcgtgacgc tgctcggcat ggacgtgggc tggttcctcg
     8761 gcggcgtgct cgtcatcgag atcgtgttcg gcatcccggg catcggcggg caggcgtatg
     8821 cggcgatcca gacgcaggat ctgccgatga tcatgggtac agtgctcttc gccgcgctgc
     8881 ttgtcaccat cagcaatttc gtagtagacc ttgcctacac gtggctcgac ccgcgcgtga
     8941 agtacagctg atctgacccg gcgttccggc gccgggacca cgacccgtat ggctggctcc
     9001 cgacagacag acccgttcgg cccggtgaga tcacggctcc tcgccgtggc ggagtacctg
     9061 cgcgcgcgtc ctgtccagga cttcgtcgca ggtgcggcgc tcctcatcat cgtgctcttc
     9121 ccgttcgcct acatcgaccg ccgtgagcag ctgatcggcg tccccgccgc gatcgccacg
     9181 ctgatcatca tcggcggcgc gctcggcggc ggtccgcgcg tgggcgcagg cctcgccttc
     9241 gtcggaggcg tcgcctacga cgtcctcgtc atcaccgacc gctggctgat cgccggcccc
     9301 accacgatcg tggtcgtgat cctctggctc gcggccgggt tcggcgtcgg gctcctgggg
     9361 gaccggtacc gcggccaggt ggcgcgcgcc ctcgaccagg cgcacgactc gcgtgacgcg
     9421 ctcgaccggg tggtcggggc gacgccgctg ttccacgccc gcggcggccc cgcggcggtg
     9481 gcccgggcga tctgtgacgt ggccctcgag accttcgact gccagctcgc cgcgctcttc
     9541 gcgatcgagg gcgaacggct gcgactgctc gcccgttcgc cctacctcgc caggaccgcg
     9601 gagcggctga tcatggagcc gtcgttcgag ctgcaacagg agctcgccga gaacctgctg
     9661 ccccagttcg tccacgacgt ccgagaaccg ttcggcccgc gcatgccgcg cgccgtcacc
     9721 tcagaccccg accaggtctc cgcgatccgc gcgccggtgc tgctcgacga ccagcccgtc
     9781 gcgctgctcg ccctcagctg gtcgcgccgc atgcccgagc cggaacgcgg cgacctcggc
     9841 gtggtgcagc gcttcgccga gcacgccgcc gtcgccctcg cccaggctca gcgcgcccag
     9901 gcccagcagg acgtggccgc gctctaccgc cggttccagt ccagcctgtc cccggtggtc
     9961 aaagtcgagt ccgaccacat caaggtcgcc acctactatc ggccgggcga agcgcgcatg
    10021 ctgctcggcg gcgacttcat cgacgccgtc acccggccca acgggtcggt ggctgcggtg
    10081 atcggggacg tcacggggca tgggccggac gcagcagcgc tgggggccag cctccgcgcg
    10141 gcgtggcgcg ccctcgccct ccgaggggcg tccctgcagg cgaacatgaa gacgctcaac
    10201 agcctgacgc tggacgagag cgaccgcgcc gaagagggcg agtcgggcct cggcctcctc
    10261 gccaccgtct gcgcggtcga gatcgacgga gccggccggc gcgcccgctt cgtcaacgcc
    10321 ggccacccca tgccgctgct gctcggcgcc gaggtcgtct cgctcgagca caccccgggg
    10381 atgatgctcg gcgtggagca gtacggcgac tgggcgccgc acgaggtcac gctccccgaa
    10441 gactggggcc tgctgctgta cagcgacggc atcgtcgagg cccgtttggg tcccggcacg
    10501 cgctcccggc tcgggctcga cgggttgcgg gcggcgctcg ccgacctgtg gttgcggcgg
    10561 gcgataacgg cggcggacct ggagcgactg gtggagggcg tgcaggccga caggcaacac
    10621 gtgctcgagg acgacgtcac gctgctgctg ctctcacacg acggccagcg gcacgcgcac
    10681 ggtcacgtga tctgacggcc ggcggcggcg cggccgccgg aggccggcca tccggcccgc
    10741 cgccgtccct cagtctgcga cggcggcctc cgggacgccc gactcgagca ggtccggcag
    10801 cgcgcgctgc agcatgaact ccagcacggc gtaggcgcgg cgcatgtcga agtcgtcgtc
    10861 gatggcctcc tggatggcca ggccgtcgac gaccgccgtc atgagctcgg cgacgccgag
    10921 gagcgcctcc tcgtccggat gctcggggca ggccgccgcc ggctccccct cgtggcgcag
    10981 ccagtctcgc ttgatctggc ggtacgacgc gtaggcgcga gccatgcggc tccggaagct
    11041 ctcgttgcgc agggcgtacg ggaggatgtc gaagaagacc cggaacgagc gcgagagact
    11101 gatggtccgc agcccgtcca cgaaccgcgg cagtcggtcc tcgctcgtga ccgtgctcat
    11161 cgcggctgcg gcgtcctgct ggtcgtcgcg gaacacggag tcgatcatcg cctcgacgag
    11221 gccctccttg ttcccgaagt agtactggac catcgccgag ttctcgcctg actcctgggc
    11281 gatggcgcgc agcgtcaggc cggagaagcc cttctcgacg agcacgcgct gcgcagcggc
    11341 caggatgttg agcgccggcg gcgacatgcc ctgccggggg tcgccgccgg acggcgcctg
    11401 acggctcgac gcgacgtggc cgttcgaggc cgccggagcg gcggcgacgg gacgtccgac
    11461 gcttgcgtca cgctcggcgc gggccatccg tggtatccct cgtgtagttg aagttaatca
    11521 cttgattgat tagactcctt caagccgcac caatcaaccg tgaaaatcag cgagaggaac
    11581 gacgatggcg gagaccaaga ctgaggtcaa gacgctcggc tacatggtcg acggcgagtg
    11641 gcgcgagtcc aagagcgaca cgtgggtcga gatcaccaac tccaacaccg gcgaggtcat
    11701 cgcgcgcacg ccgatgtgca ccgaggccga ggtcaccgag gccatcgagt cggccaatcg
    11761 cgcgtacccc gcctgggcct ccatgcccat ccagaagcgc accgagatcc tgttcacgtg
    11821 gcgccccaag ctcatcgagc gcatggacga gatcgccacg ctggtcagca ccgagctcgg
    11881 caagaacctc gacgaggcgc gcggcgaggt catcaagatc atcgaggcca tcgacatcgc
    11941 cgtcggcgcc ccgatgctga tgaagggcga gagcctgatg aacgtgtcca ccgggcacga
    12001 cacggtcagc taccgcgagc cgctcggcgt gttcgccggc atcgcgccgt tcaacttccc
    12061 ggccatgatc ccgttcggct ggatgctgcc gatctgcatc gcctgcggca acaccttcgt
    12121 gctcaagagc gcgaacttcg tgccgctcac gagccacctg ctgctcgaca tgctctacga
    12181 ggccggactg cccaagggcg tcgtcaacat ggtgaccgcc gagaaggaag aggccgaagt
    12241 gctgctcaag cacccggcca tccgcggcgt gagcttcgtc ggcagcacgc cggtcggcaa
    12301 gcacgtctac tccatcgccg ccgctcacgg caagcgcgtc caggcgcaga cgcaggccaa
    12361 gaaccacggc ctcgtgctcg ccgacgcctc gctcgagcgc accgcccgcg gcatcatcaa
    12421 ctccaccttc ggctgcgccg gtatgcgctg catggcgctc cccgtgtgcg tcgtcgagga
    12481 gtcgatcgcc gacgagttcg tcgcgctcat gaagaagttc gccctggagc gctcggtcgg
    12541 cgacgccacg ctgcccgcga ccgagctcgg cccggtcgtg agcaaggcgc accagcagtc
    12601 ggtcctgcgc tggatcgaga cggcgatcga ggaaggcgcc gagctggtgc tcgacggccg
    12661 caacgtcacc gacggcctgc cggacgagct caagggtggc cactacgtcg gcccgacgat
    12721 cttcgaccac gtgaccgagg acatgctctg cggccgcgag gagtgcttcg gcccgatcct
    12781 cttcgtcaag cgcgtcaagg acttcgaaga gggcatcgag ctcatgaaca acaacccgta
    12841 cgccaacggc tcgtgcatct tcaccgagag cggttaccac tctcgtgagt tcgcgcgccg
    12901 cacgcacgca ggcatggtcg gcatcaacgt cggcatcccg gtgccggtca gcttcttccc
    12961 gttcgcgggg cacaaggaca gcttcttcgg cgacaaccac gtcttcggca gggacggctt
    13021 ccagttcttc accgagacca agtgcgtcac cacgcgctgg ttcaccgagg ccgacaagaa
    13081 gcagaagcag gtcagcacct gggagggcac cgtcaaccgc tgaggcggct gacgcgccgg
    13141 ccgcggatgc ggccggcgac cgcttcccga agcgctcggg ggcggagggt gggcgatcag
    13201 cccaccctcc gccccctttt tcgtgcgggg cccctcagcg cggagccacc gccgaccgcc
    13261 gtcgtggaat cggcggcgac cgggtaggaa cgtcggcgag acgaccaaag gagaacgacg
    13321 tggcacacgc attcccggag atcacggcgt acgggcccgc gctgcgcttc gcgctcgagg
    13381 cggagcaggc atgcgccgac ttcgccgccg cggccggcgt cctggcgccg acgcaggagt
    13441 ggcaggacaa gctggaggag gtcgtctgca cccacgacga ccgcgtgcag aaactgatgg
    13501 ccaagcaggg gcagctcggc gacaaggcgt cggcgccggc aggcaccctc aagcgcgagc
    13561 cctacctggg tacgctcgac gcggagccgg ccatcagctg gccggccgtc gtggtgcagc
    13621 tcgcccaggc cgaggaggac tccgcgcgtt accacgagga gttcgcgatg cagtgtgccg
    13681 acacgctgga gctgcaagcg cgcctgttcg gcaaatcggc gcagcagaac cgggcatacg
    13741 ccgccgagct tcgggagctg ctcggctagc atccgagaac ggcgcgtgga cgaagacgcg
    13801 atgcgcggcg cggacgagtt gcacccggca cggagtggtc tgcccttccg tcgcgctggg
    13861 atctctctgc gcagcggcag gcgcatccac cggatcgaat tccgtggcgc gatcgctcga
    13921 gagggcggtg ggccggggcg ggcgccggga ggcggcaccc gccccggcct gctggcgagg
    13981 gcgcttgcgg tcgccctcgc ggagctcccg gcgagcacgc ttgcggacac gctcgccaga
    14041 gccggacggc gaggccgggg aggccgtcgt ccggctgccg gggcgcgtac ttgggggacg
    14101 ccccgggtgg tgcggggagg cggggcgggc ggcgcgtcac gcgtgaccgc ccgtcccgtc
    14161 tcccctacgt ggccgcctcc ccgggcggcg ggtcgccact ggcagtcggc gtgggatcgt
    14221 cggcggcggc cggcgcggcc acacgggcgg cggcgggact gacgaccgga gctcggccgc
    14281 ccggcaggct ccggccgcgc acggcggcca gcagcgcctc ctcgccggcg aagaactggg
    14341 cgccgccctc ggcgaggccc gcgacggcgc cgacgaagtc gctggcgcgc actccttcgt
    14401 cggcccagac ggggatgccg gcggcgagcg cgtcgcgcgc ggcctcgagg ttgcgcgcgt
    14461 taccgtggct cacgggcacg gcggtcacga cgacgaggtc cgccgcgcgc atgagctgca
    14521 gattctccgc gtgcgcctct tcgcccaccg gcgagaacgg cgcctcgacg gccatgtgca
    14581 ggccgagctc gctgccggtc tcctcgtcgg tgtcgagtgc gttgaggacg ccggcggtga
    14641 cttcgtagcc ggcgtccacg agctcgcgca tcatgccggc gcccgtcccg ccgccgcaca
    14701 cgaggtggac gcggccgcgg cgctcgccgc gcagccgcgg tgagaacgtc aggtacgtac
    14761 gcccggcgtg ccggtgcagg taggcgcgca cgccgtagac ggcctccacc acctcgggcg
    14821 ccagcacctc gtccggcggc ccgaacgccg ccacctcgcc gtcactcagc acgagcagct
    14881 tgtcgaagta gtgcagcgcc agattgaggt cgtgcaggat gcagaggacc gtgaggccgc
    14941 cggcgttgag ctccttgagg aggtcgaaca ggcggagctg gtgagagacg tccagggccg
    15001 aggccggctc gtcgagcagc aggagcggcg cctgctgcat gagcgcctgg gcgagcaccg
    15061 cgagctgctt ctcgccgccg gagagctccg agaaccggcg gctcgcgagc tcggcgaccc
    15121 cgagacgcgc gagcatcagc tcggggtcgc cgcacgccgc cgggtccttg ccgcggcagt
    15181 aggcccccat ctccacgact tcgcgcacgg cgaggtcgaa gtcgaggtcg aagtgctgcg
    15241 gtacgagcgc gacctcacgc gccatggcct tacgcttgta ccggcgcgta gccaggccct
    15301 tgaccagcac ctcgccggcg accggctgat ggatgccgtc gatgaggtcc accagcgtcg
    15361 acttgccgct gccgttgggg ccgacgatgc cgacgaagtc gccggcctcg agcgtgaaac
    15421 tgatgcgccg caggacgggc cgctcgtcgt aggcgaagtc cacgttcttg agcgcgacga
    15481 gcggcgcgtg ccgagtctcc cgctccgggc tgggctcggg gacggcgggg tggtccgggg
    15541 gcgtcgccat caggcgcgcc tcccgcgccg caggaggtac acgaacagcg gcgcgcccac
    15601 gaaggtggtg accacgccca cggggatctc gttgggagcg atgaccgtgc gcgagagcag
    15661 gtcggccacc accatcatgg aggcgccggc cagcagcgag accgggagca gcccgcggtg
    15721 tcccggtccg accagcagcc gcacgatgtg cggcacgacg aggccgacga acccgatgat
    15781 gccgctcacg gagacggcca gcgcagtcag gaccccggcc gtcaccagca gcatccgctt
    15841 ggtgcgctcg acttccacgc cgagctcgag cgcgcgtttc tccccctgca gcatgatgtc
    15901 catctcgcgc gtgaagaaca gcggcaggac catgaacggg atcgtcacca aggcgatgcg
    15961 cacgtccggc cagccacgcc cggagaagct gcccatgagc cagtagatga tcgccgtcat
    16021 gtcctggttg gccttgatca tgagcaccgt gacgacggcg ctggcgaggt aggcgatggt
    16081 gacgccggcg agcaggagga cgaacacgtc gatgcggccg cgccggcgcg ccgccatcca
    16141 cacgatggcc agcgtgccga gcccgccgac gatggccagt accgtgatcc acgacaggtt
    16201 gctgaggtgg aggacgatgc cgatggtcgc cccgagcgcg gcgcccgacg agacgccggt
    16261 gacgtacggg tcggtcagcg ggttgaggaa gaggtcctgg aggatcacgc cggacactgc
    16321 cagcgccgcg ccgacgagcg ccgcggtgag gacgcgcggc atgcggatct gccagaagat
    16381 ggcgttgtcg aggtcgcccg ggaccggccc cgcgaagagc agttgccaga tccgtcccac
    16441 ggacagcggg gccggtccca gagcgagcgc cagcacgagg cacaccgcga gcatgccgga
    16501 gaccaggagg atccccggcc acgtgcctgt gcgggcgagc ttgctcactg cgccgtctca
    16561 gcctccggat ggatgtagcc ggccagcgcc ctgaggcctt ccgccaggcg cgggccgggg
    16621 cgggcgatga tgctgtcgtc gatgatgtag accttgccgt ccttgacggc ggtgagctcg
    16681 ccgaagccgg cgcgcgcgtc gatctcgccg ggctcgctca tcgagccgga atcggcgatg
    16741 tagacctgcg ggtcggtctg caccagcacc tcggtgctga agttcgggaa cccgctcccg
    16801 gccgtggcgc cgatgttctc gccgccggcg atggtgatca tgtcgtcgat gaacgtgtcg
    16861 gcgcctgccg tcatgagcgg cttgccgtag atctcgagga aggtcgtcgc cgcgggcagg
    16921 tcgccgaccg cgtcctggac ttcctgccgg gcggcggtca tctcggcggt gaccgcggcg
    16981 gcgccgtcct cggtgccggc gagcttgccg acgttctcga tggtggcgat gacgccgtca
    17041 taggtcttgg ggtcgacgac gtagaccgtc atcccgagat cctcgagctt gccgcgcagg
    17101 tcggcctgga tcccgccggc cgcgaggacc aggtccggcg cgaacgaggc gatcttctcg
    17161 acgttggggt tggcgaagtc gccgatcttg ggcagcgccg tggcctcctc gggatagtcg
    17221 tcgtagctgg tgccggcgac catcttgtcg ccggcgccga cagcgtaggc gatctcggtg
    17281 ttggcgggcg cgaggctgac gatcgcctcc gccggctgtt cgagggtgac ctcgacgccg
    17341 gagtcgtcgg tgacggtgat ggggccggcg gccgcggccg cggacgggga gctcgaggcg
    17401 gcggtgtcgt cgcccgagcc gcaggcgctc agggcggcgg cgcccagggt gagcacggcg
    17461 acgagcagga gcgccgcggt gaggcggccg aaacggatgg tggtcatgac ggtggtgact
    17521 cccttcgatc tttgacgaga cgagaacgga cgatgtcgac gagctggggt gacgctccgg
    17581 ccggcggggc ggcgggcgtc acggtcgccc ccagccgcat gtcgcggagg aggtggtggc
    17641 cgtcgcgctc ctcgacgccg tcccagacga gcgggacggg gcgcgagaag agcggagcgg
    17701 cggtgaccat ggtgtggtac tcaccctcct cgccgcaggc gtcggcgccg gcggcctcga
    17761 gacgggcgat gacggcgtca tccagcacga ggccgaggaa ctcggggctg agcttggagg
    17821 agtcgacggc gacgatggtg gcgcgggagt cgccggcgag gaactcggcg agcagccggc
    17881 ggcgcggctc ctgccagagc ggcagatggc agccgaggcc ggcgacctcg cagacaccct
    17941 cgacccaatc acggtgcgcc tgcaggtcga tgtcgccgaa cacgccggcc tcgatgccct
    18001 gcgcgcgcag ctcgtggagg agcgcgacga aggtggactc gtagccgtcc cacgtggtgg
    18061 cgcgggtgac gagcggtacg ccgagcgcgg cggcctgctc ttcgagcagt gcgagcggca
    18121 ggccgtggcc gcggctcacg ccgccatcct cgtgcagcat gcagacgagg gcggccgccg
    18181 cgccgccgcg ctccacggcg cgctgcagcg cgaggtacgc gtccttgccg ccgctccacg
    18241 agcagaagaa acgggaagcg gcgagcgacg cgccggcgga cctgccgttg gcggacggga
    18301 cgtcggtccg cggcgcgtca ctcgccatgc tcgccctccc tggcgctgcg acaggagggg
    18361 cagatgccgt acacctcgag gtggtggccg tagatgctga acccggtggc cgccgccagc
    18421 ctggcctcga gctcggcgac gtcgccgtcg ccgtcgaagc ggaccaccgt gcggcagacg
    18481 ccgcagatga gcgggtggct gtggccgatg ggcggggcga tgtagcagat ctcgccgcgc
    18541 ccgtcctgca cgcgggccag cagtccgaga tcgctgagga gctggacgct gcggtacacg
    18601 gtggcctggc cgacgccgtc gctgcgcagg gcgtcggccg cctcggtgat ggtgaccgcg
    18661 cggtcggcgc cggcgaagaa gtgccagatc tggcgccgct gccgcgtgaa gggaatgccc
    18721 ttctggcgga agactgcctc gatctcgtgc gccggcttcg ggtcggtcaa cgttgtgctc
    18781 ccggggaatc cagttgagaa ccgttctcaa cggtgttgag aaaagctatc aaccacatga
    18841 cggcgcgtca acctcttcgg ctcgaccgtc tccggtcgct gccggaccct acgcgagacc
    18901 agagacgccg agagcgatat cgtgagcgga tcagggtcga cgccggccgg cgaggtcgca
    18961 tgccatcccg ccttcgcggt cggccttcaa cacggcgcga acggaggagg gcgatgatct
    19021 gtccggagtg tggcgcggag tacgtgcccg gattcacccg ttgcgcggac tgcctcgtcg
    19081 acctggtcga gccgggctcg caggacgatg cacccgagcc ggcgcgcggc gaggccctcg
    19141 ccgagccggt ctgcgtctac cgcgccaccc agcgtggtcg tcttccgctc gccgaatcgg
    19201 tcttgcggtc cgccggcatc cccttcgtcg cctcaggaga gcacctgcag cagctcacgg
    19261 gtctcgacgt cttcggtcct gcggagctct acgtggcggc ctgcgacgta gacgacgccc
    19321 gcgccgtgct cgccggcctc gacggggagc agtgagctcc cgctgacagg cccggccggc
    19381 taggcgagga tctcgcggac cttggcggcc acggccacgc cgagcagctc gtgctgcgag
    19441 gcctcgaggt gccagccgtc gagcgcgctg gtcgtgatga gcttcccggc gtcgagccag
    19501 gggacctgca gggcggatgc gacgcgcgcg aactcccgcg gcagcgcgcg cgacttctca
    19561 tccgccccca cccagagccc gggacgccag ttgttctcga agggaccaac cggcggcggg
    19621 cagaccagca ggagccgcgg cgccgcgcct gcggggccga gctcgcagcg ctgtacgaga
    19681 tgagccagct cgccggcgcc gtcggccgcc tccgccgccg agatgtgcgc gtagctgatg
    19741 tcgttggtcg ccagcatgag gacgacgagg tcgagcggcg cgtgggactc gagcgccgga
    19801 gcgaggagct gcctgccgct ccggcgcggc agcagagggt cgtcgtagac cgtcgtccgc
    19861 ccgccgagcc cctcctcgat cacgtgatag ccgccgccga gctctcgctg cagcacgccc
    19921 ggccagcgga cgggccactc gaagcggcgc gcgtcgtcgg cggggtcggc gccccaggtg
    19981 ttcgaatcgc cgtagcacag gacctgcctc atcttcacct ccactgcccg cgatcgccga
    20041 actcaggccg ccttgggatc cgactccagc agacccagga ggttgccgtc gggatccttg
    20101 acgtaggcga gccagcccac cccggtcacc gccatcctgg gcagggcgac gaccgcgccg
    20161 agctcgtcgc ccttcgccag tgtcgcatcc agcgagtcga ccgcgaccgt gcacgggaac
    20221 gcgttgagga ccggtgcatc ggccggtccc gggatcggcc ggcgtaccag cccgccgtcg
    20281 atcccgggct cgtcggcggg cccggtcccg atcagccagt actcggtccc ctgcgtgggg
    20341 gtgaacgtcc agccgagcag gccggagtag aacgcgatga gctgctccgg ctcagtcgca
    20401 tggatctcga agtggacgac gcgtgccatg gcacctccct cgtcagacgg cggcccctga
    20461 tcagccgccg tgctcgagct tgcgcgcaga acatgacggc gccagcgtcg ccgccatgag
    20521 accgtgtccg ggaagcggct ggaaaccggc gctgcgatac accgcctgcg cggcggtgtt
    20581 gtccacgccg acctcgacgc gcatggcgcg catcccgagg tcctcgcagg cccggcgagc
    20641 cgcggcgagg gcggccgtgc cgagcccctc gccgcgcgcc tccgggcgca cgtagaagtc
    20701 gtcgaccaca gccgtcatcc ccccgaactc catcgcgtgg acgaaggtca cgacgatgta
    20761 gccggcagcg ggctcgatca gccacacgcg tcccagccgg gcgtctgcca gcagcgacgc
    20821 gaacccggcc gtcgccctct cctcgtccag cgtgaagcct gcctccgcgt agaacgcgcg
    20881 catgagggcg accagctccg gcacgtccgc tcgacccgct ctgcgcatcg cacccccgag
    20941 cgcttcctta ccagtagtca ggacaggaag tcctcgcgca gcggcgagaa gacctcgacc
    21001 gccacggcgt cctccagcac ctcggcgccg tggggcacgc cgccggggat cgaccagctg
    21061 tccccgggcc cgacgtcgtg ggtctcgggg ccgatggtga gcagcagccg gccgctgacg
    21121 agatagcccg tctgttcgtg cggatgctcg tggacgggca gcgggtagcc cgcctgcagg
    21181 cggaactcgg ccatcagcgt cgccgcgccg tggctgagtg tcctgcgccg gatgcccggc
    21241 agagcgtcca gatagccgtc gtcactggcc ttgaggaaca ccgcggtcct gcccatcagg
    21301 ctttcgcgcc agcacgtaca cgcgggagaa gagaagctcc acgagaccgt cgccgcccgc
    21361 cctggagctg aggtcgtcca ggatgagctc ccgcgccgct tgcgcatagc cggccggcag
    21421 cggcgcatcg tagcactcgg gattgaggta ccccgcggcg gcccccgact cgaacatctc
    21481 cagcgcccgc tcgggggtcg cgtgctcgcg aagctcctcc atctcggccg ccaccacgga
    21541 gaacccggcg gcttcgaaca gccgtccgta ctcctcgccg gtctcgtaga aggtccacgg
    21601 cgtgcggaag tgggaccacg tctctgcggt gcgaggatcg ccggcgagcc tggccacggc
    21661 gtgcacgaac tgcgggcagt actcctgtcg ggcgggcgcc tgcatggcca tgcgcccgcc
    21721 cggcctgagc gccgcgagac agttcctgag cgcgagctcg ccgtccttga accactggaa
    21781 ggccgagttg ctgaggatga cgtcgaacgc ctccggcatg tccagggcgt ggacgtcgcc
    21841 ggtgacgaac tcgatcgtcg ccggcgccgc ctggcgcgcc agcgcgatca tctccggcga
    21901 gagatcgatg ccgaccacgc ggccgtccgt gagcacgcgg atctcctgcg cgaggtggcc
    21961 cgtgccgcag ccgaggtcga gcacgtcgtc ggtcctctcc acgcccagca tccagaacag
    22021 gcgctcggcg gcggacttct gcgccacggc cgtctggcgg tagatacgcg cgatggacga
    22081 gaaatcgctc agcgagactc cccgagacgg gttccgtgcg tcatcatagc ccgttgagcg
    22141 gcctctccgt cagcccctgc gtggggggcg gggaacgagc agcgaggtcc ggtgcttgta
    22201 ggcttcgtag tcgagctgac cgccccactt cttgtcggcg gacttctcga gcagcggcac
    22261 gccgctcacg cgcagcagca gcagagccac gaagacgggt gagatcagcg tgatccactg
    22321 ccagccgcgc agcaccggaa gggcgacgat cgccacgccg acccacagca ggatctcgcc
    22381 gaagtagttg ggatggcgcg accatgacca gagcccggtg tggatgaacc ggcctttgtt
    22441 ggcggggtcg gcgcggaagc ggctcttctg ggcatcggcg acgacctcca ccccgaagcc
    22501 ggccacccac acgagcagcc cgatggccgt caccgtgccg atgccgacgc gctccgtcgt
    22561 ggtgatggcc gccagtgcgg ccgcgagcgt cagcgtcacc caaagcccct ggagcgtcca
    22621 cgtgttgaga aagcggggga acgacggctt gatggcgtcg aagcgcgtgt cggcgccggc
    22681 cctgcggatg cgcaggaaga ggtaagaggc cagccgcacg gcccagatga cgaccatcgc
    22741 gagcagcagg tacgagcggg cgtccggcgc gtcggagaac agcaccgcga cgaccgcgac
    22801 ggtgatgtac gtcaggccgc cggtgaggtc gaagaaccgc tcgctctgga gcacgtacgc
    22861 ggggacgaac gcgacccact ggatggcgaa cgcgacgatc acgcagagcg cgaacaccgg
    22921 gaggctgccg acggtggatc cgccctggct gccggccagt gccaccaggg cggcgatggc
    22981 gaggacgacg ggcagggcga cgagagcgtt gcggtccggc ttcttcatcg cgtcatccta
    23041 tcctgtcgac cgcgtgtcga cgcgcgtgct ccgggggaag gcgcggtctt ctcgaggtcc
    23101 agaagccagt cgttgctgcg catcacgacg cccgatgggt agtcgacctc gacctcgccg
    23161 agcagctcga agccgagcct gcggcagagg gcgttggagg cggcgttgcc gaccgtttgg
    23221 atggcgtgca gatggcggtg acggccgcgg cgccgggcat cgtcgatcat gagcgtcgcg
    23281 gccgccgtgg ccacgccgcg accctgcgcc tccggcagga cgttccagcc acactcccac
    23341 accagctcgc cgtgccactc cgactcccag tagccgaccc agccgaccgc ctccgctgca
    23401 gggccggcgg tgacggcgaa cagcccgtgc ttctccgggt cggcggccag gtagcgctcg
    23461 tgacgggcgc ggatggcctc ctgcgactcc gggccgccga gatggcgcgt catctccggg
    23521 tcgccgagca gccgctgaag cagccagagg tcgccggccg cccagagccg cagggcgacg
    23581 tccgccctcc gggagtggcc gccccccgcc gcggtcacca gccgcctgcc gccccggcgc
    23641 ccagggtgac ctcgaggtcg cggagcaggc cgtcctccag gtcgtagatc cagccgtgga
    23701 cggccaggtc cgcgccgcgc tcccacgcgc tctggacggc ctcggaccgc gcgaccgcca
    23761 ggacctgcgc ggcgacgttc agctcgcaga gccggtccca ccgcgcggcg tcggagggca
    23821 acgcgtcgag ctcatcgcgg tgctccccgg cgaggttccg caggggctgc agccagaggg
    23881 cgacggcgcc ggcctggtgt ccctcgagtg cggcgcgcac ccccccgcag cggtagtgcc
    23941 cgcacacgat gacgtgacgc acgccgagcg acgatatcgc gtactcgatc accgccatcg
    24001 cgttcaggtc ggtgtccggc accacgttgg cgacgttgcg gtggacgaag agctcgccgg
    24061 gcgccaagcc gacgatctcg ttggcgggga cccggctgtc ggagcagccg atccagagca
    24121 ggtcgggacg ctggaggtgc gccagccgcg cgaagaactc cgggtcggcg ccggttcgtt
    24181 ccgcggccca cgcccgattc cgcgcgaaga ggtcggagag gtacttcatg cggccgtcct
    24241 tcccatcacg gtgacagctt agcagcggtt tgtggagcaa gcggcgtcgc gctatgaatc
    24301 acccatgcac gagacgcgcc tgctgatcat cggcgccggc gtcgccggac tcaccgcggg
    24361 atgctacgcg gcgcggtccg gctaccggac gaccatcctc gagatgggcc agacgcccgg
    24421 cgggctctgc acctcgtggc gcaggaaggg ctatctgttc gacggcagcg tcgcgggtct
    24481 ggcgggaacg tcaccggagg cgccgatcca ccgcctctgg cgggacatcg gcgtcgccga
    24541 ctactgcccg ctctacgacc ctgtcgactt cggcacggtc gtcgccccgg acggcagggc
    24601 ggtcaccgtc tccaccgaca tcgaccggct cgaggcgcac ctgcgggaga acttcccgcg
    24661 cgacgccgcc gccatcggcg agttcgcgcg ggcgctgcgc gcctgcctgc acctggacat
    24721 cccgtttcgc gccgagaccg gccttgcggg gatcgtcgcc ggcgcccaca cgggcgtggc
    24781 ctcgctgcgc agcctgccgg cgctggtcaa gtacggccgc ctcacgctgc gccggttcaa
    24841 cgcccggctg cgggacccgt tctgccggca ggtgttcgac aacctcgtcc acttcggcgg
    24901 tcaggacgtg cccatgctca ccgtcctgct cccgatcgcc tacgcccacc gccggatgac
    24961 cgggatcccg cgcgacggct ggctcgcctt cggccgggcg atcgagcggc gcttcctcga
    25021 gctcggcggt gagatccgct acggcgccaa ggtcgagcac ctcgagaccg gcggcggcgc
    25081 gg
//
LOCUS       DASUMX010000121         7574 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1902660, whole genome shotgun sequence.
ACCESSION   DASUMX010000121 DASUMX010000000
VERSION     DASUMX010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 7574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7574
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1902660"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /gene="hemC"
                     /locus_tag="VFZ86_06040"
     CDS             complement(<1..663)
                     /gene="hemC"
                     /locus_tag="VFZ86_06040"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007953934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEX5641886.1"
                     /translation="MNSRAPLRDVVRIGSRGSRLALVQAEWVRDRVAAHHPHLTVEIE
                     IINTKGDKILDAPLAKIGDKGLFTKELETALLDGRIDVAVHSAKDMPTAIPEGLALIA
                     FTEREDVRDAFVPNPALLEARGPDAGALTLDDVPRGARVGSSSLRRRSQLLALRPDLD
                     VVDIRGNVETRLRKLVEEDMAGTILAAAGLARLGRSEVIGFAFAFDEMLPAVGQGALA
                     IEA"
     gene            complement(660..2105)
                     /gene="hemA"
                     /locus_tag="VFZ86_06045"
     CDS             complement(660..2105)
                     /gene="hemA"
                     /locus_tag="VFZ86_06045"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEX5641887.1"
                     /translation="MHVVVAGISHKTASLELRERLALDEAAALRLSQELLDDGVACEA
                     VALSTCNRTELYLYGRDSLEARHAALARLARHAGVAPSELEPSLYSYSGDGAIAHLFR
                     VTSSLDSMVVGEAQIVAQLKAAYQQACDGGCTAVVFNRLFRHALEVGKRVRTETAIGE
                     RPVSVSSAAVELALQVFGKLRDHTVLIVGAGETSELTATHLKAHGIEKILVANRTLDT
                     SMDLARRVGGEAVRFEDLEHHLAAADIVISSTSAPHYVVTRETVERAFKRRRHRPMFL
                     IDIAVPRDLDPEINRVRDAYLYDIDDLQHVVEHNRHEREKEAEHAERIVEQELASVND
                     WLRSLEVVPTIATLREAVEDIRRGELERLSNRLSDLTDEQRARVDMLTTSIVNKILHV
                     PTVKMKEVAGRDQCYLYVDAVRTLFDLDTDEGGEERPAGEVAPDGAGSEADQGAGERS
                     DAESAESPASSTPPAPGGTVHHLRGTGTEDA"
     gene            complement(2110..2937)
                     /gene="ccsA"
                     /locus_tag="VFZ86_06050"
     CDS             complement(2110..2937)
                     /gene="ccsA"
                     /locus_tag="VFZ86_06050"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEX5641888.1"
                     /translation="MEQTSVILIWLAYLLYAAAFAVFLIGFFTQRPWTNRVGIVVAAA
                     GLVAQTLGLVLRGVSAGHFPFSGLYESMVLVAWAIVLVWHVLEAFTKIKAVGLYVMPV
                     VLVLLTVALVSYDPPAGLVPALRSDVVIVHVAVMLVAIGCLYVAGGAAIIYLIEETLL
                     RRRKPGGVLGRLPSLAALDRLIYHATLLGLPFLTMGMAAGVIRAETFKVPGWPTDPMV
                     LLSAAAWAVYLALIAGRVRGDWSGRSVSWLAVAGLVLLLVIRFAAVPYLSGFHTYGG"
     gene            complement(2982..3161)
                     /locus_tag="VFZ86_06055"
     CDS             complement(2982..3161)
                     /locus_tag="VFZ86_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641889.1"
                     /translation="MTIEEGVKYVAAAYAIILAVLVIYYVMSARRVATLQRDVQLLED
                     EAKRLKAPAGEAGAE"
     gene            complement(3166..3849)
                     /gene="ccsA"
                     /locus_tag="VFZ86_06060"
     CDS             complement(3166..3849)
                     /gene="ccsA"
                     /locus_tag="VFZ86_06060"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEX5641890.1"
                     /translation="MKKLLSAPALFIWASVLLIIGLLLAFWYSPTDASSMGFSQKIFY
                     YHVPIAAAAFVAFGVAFVAAIAYLRTQDLKWDRLGYVGVRIGLLFSILVMATGMIWGK
                     AAWGVWWAWEPRLTTYLLACFLYAAYWVLRSVVDEEDRRATYAAVFAIIAFIDVPITF
                     FATRFLPEGLHPVVVGPGGGGMEGSIAVSFAICMVGMLLLMAAIIRRDYEIELIRDRV
                     TDLKTSLGG"
     gene            complement(3852..4538)
                     /locus_tag="VFZ86_06065"
     CDS             complement(3852..4538)
                     /locus_tag="VFZ86_06065"
                     /inference="COORDINATES: protein motif:HMM:NF015344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HEX5641891.1"
                     /translation="MSGWSQFKAILRKDLIRELRTRDMLVSMILFVLLAMIIFHYAFT
                     VKEGADLTYFTGGMLWVTFIFAMLLGLNRSFAQEKDEHCLDGLLLCPVDRVTIFFAKT
                     AGNLVFLLIIQAVAVPVFALFFIERNFLGDLLPFLIVLALADLGICALGTLLATISMN
                     TRSRDLLLPILFLPLIVPVLIAATGATTLIFAEGVSFGSLVARLLLLLGFDAVFLVAA
                     WGTYDYAIGE"
     gene            complement(4541..5287)
                     /locus_tag="VFZ86_06070"
     CDS             complement(4541..5287)
                     /locus_tag="VFZ86_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009139408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5641892.1"
                     /translation="MSDEHDRQDGAVSLEIEVEGLRKTFGRREVLKGLSFGVPRGGFL
                     SIFGPNGAGKTTTLRVLATLLASSGGSVKVAGHDVREDPMPVRKAIGFISHNAMLYPD
                     LTAQENLRFYADMYGVDDREARITELLERLELSARRYDVVRTFSKGMRQRLAIARAVL
                     HRPRVLLLDEPHSGLDPRAVDILDGLLTEIRDEHTFVMVTHNIAKGLEWGTDLMIIEG
                     GRIAYEHAAGVDHDSFSAVYREHVHDGSVL"
     gene            complement(5299..7293)
                     /locus_tag="VFZ86_06075"
     CDS             complement(5299..7293)
                     /locus_tag="VFZ86_06075"
                     /inference="COORDINATES: protein motif:HMM:NF027651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HEX5641893.1"
                     /translation="MRDLGTYSLVATLILALGAAWLLALGSWSERKDLIRYGYYAVYG
                     FFLTTVIASAVLLQAFLKGDFSFAYVVENSDTSLSVFYRIAGFWAGQSGSFLLWLLCL
                     AIAVIVIAVIDLNRLERLTGGAVAVLAVISAVFAALMIFDSGSNPFLAAEAGAMPFGL
                     NPLLLHPGMVLHPPALFIGYVGLAVPFAFAVSTLLLGRGDKLWVQRSQKWAVFGWLFL
                     SLGIGLGAWWAYVVLSFGGYWAWDPVENTSLVPWLTATALLHAFTLYKARGLFKRWAL
                     GLAAATFFFTILATWTTRTGLISSVHAFGRNPVLIWILSTLLVVTAVGSAALIVWRWN
                     RFESRDDVESWLSRDFLYYVTNLLLTLFAVAIAFATVAVPLLMERTVGASTYDAVAQP
                     LGVVVLAMIAVCPLLAWRKTEGAELRKTLVLPTVTMLASIPLWLYLGFQSSIWGFIGL
                     LVCGFAFGAVIQFVLRSARRAAGPGNSLWSGLGKAFTGSRTRTAAYIVHMGMVLVVAG
                     LLGSTVYKVEQSTIVKVEPGETASLNGYTLTYKGMEESTGAQNSTRAEATFEVTRDGQ
                     ALGTVGPHTDVYPVSGAAVRAVILGRPFEDLFVVADEPFDGTSTTIALRIVIFPLIRW
                     VWIGSILLCAGAVVSLWPKRQKQEQEARAVRPAEADGATA"
     gene            complement(7304..>7574)
                     /locus_tag="VFZ86_06080"
     CDS             complement(7304..>7574)
                     /locus_tag="VFZ86_06080"
                     /inference="COORDINATES: protein motif:HMM:NF015085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HEX5641894.1"
                     /translation="KVGGRVLDGTIERDANGVRFTISDLTGKTETVNVLYSGQMPGTF
                     EAGVDVVVIGKYASTGALITADQLQTKCPSKYEGSASPAAQSTGQ"
BASE COUNT         1276 a         2511 c         2742 g         1045 t
ORIGIN      
        1 cgcctcgatg gcgagcgcgc cctggccgac ggccgggagc atttcgtcga aggcgaaggc
       61 gaagccgatg acctcggagc ggcccagccg ggcgagaccc gccgccgcca ggatcgtccc
      121 cgccatgtcc tcctccacca gcttgcgcag gcgggtctcc acgttgccgc ggatgtcgac
      181 cacgtccagg tccgggcgca gcgccaggag ctgcgagcgc cgccgcaggc tcgagctgcc
      241 cacgcgcgct ccgcgcggca cgtcgtcgag cgtcagcgcc cccgcgtccg gcccgcgtgc
      301 ctcgagcagc gcagggttcg gcacgaacgc gtcgcggacg tcctcgcgct ctgtgaaggc
      361 gatgagcgcg agcccctccg ggatcgccgt cggcatgtcc ttggccgagt gcacggcgac
      421 gtcgatgcgc ccgtccagca gcgccgtctc gagctccttg gtgaagagcc ccttgtcgcc
      481 gatcttggcg agcggagcgt cgaggatctt gtcgcccttg gtgttgatga tctcgatctc
      541 caccgtgagg tggggatgat gcgcggccac gcggtcgcgc acccactctg cctgcacaag
      601 cgcgaggcgg gagccacggg agccgatgcg gaccacgtcg cggagcggtg cgcggctgtt
      661 cacgcgtcct ccgtcccggt gccgcgcagg tggtggacgg tgcctccggg tgcgggcggc
      721 gtggaagagg ccggggactc ggcggactcg gcgtccgacc tctcgcccgc gccctgatcg
      781 gcctcgctgc cggcgccgtc cggagcaacc tcgccggccg gccgctcctc cccgccctcg
      841 tcggtgtcga ggtcgaagag cgtgcgcacc gcgtcgacgt acaggtagca ctggtcgcgg
      901 ccggcgacct ccttcatctt cacggtcggc acgtgcagga tcttgttgac gatgctcgtg
      961 gtcagcatgt ccacgcgagc ccgctgctcg tcggtgaggt cgctcaggcg gttgctgagg
     1021 cgctcgagct cgccgcggcg gatgtcctcc acggcctcgc gcagcgtcgc gatggtcggc
     1081 acgacctcga ggctgcgcag ccagtcgttc acgctcgcga gctcctgctc gacgatgcgc
     1141 tcggcgtgct ccgcctcctt ctcgcgctcg tggcggttgt gctccaccac gtgctgcagg
     1201 tcgtcgatgt cgtagaggta ggcgtcgcgc acgcggttga tctccgggtc caggtcgcgc
     1261 ggcacggcga tatcgatgag gaacatggga cggtgccggc ggcgcttgaa ggcacgctcg
     1321 acggtctccc gggtgaccac gtagtgcggc gccgaggtgg aactgatgac gatgtcggcc
     1381 gcggcgagat gatgctcgag gtcctcaaag cgcacggcct ccccgccgac acggcgggcc
     1441 aggtccatgg acgtgtccag cgtccggttg gcgaccagga tcttctcgat accgtgcgcc
     1501 ttgagatggg tcgccgtgag ctcgctggtc tcgccggcgc cgacgatgag cacggtgtgg
     1561 tcgcggagct tgccgaacac ctgcagggcg agctcgacgg cggcggagct gacgctcacg
     1621 gggcgctcgc cgatggcggt ctcggtgcgc acgcgcttcc ccacttcgag cgcgtgccgg
     1681 aacagccggt tgaagacgac cgcagtgcag ccgccgtcgc acgcctgctg gtaggcggcc
     1741 ttgagctggg cgacgatctg cgcctcgccg accaccatcg agtcgaggct cgaggtgacg
     1801 cggaagaggt gggcgatggc gccgtcgccg gagtagctgt acagcgacgg ctcgagctcg
     1861 gacggcgcga cgccggcgtg gcgcgccagg cgcgccagcg ccgcgtggcg cgcctcgagc
     1921 gagtcgcgcc cgtacagata cagctcggtg cggttgcagg tgctcagggc gacggcctcg
     1981 caggcgacgc cgtcgtccag gagctcctgg ctcaggcgca gcgctgcggc ctcgtcgagg
     2041 gcgaggcgct cgcgcaactc gagcgaggcg gtcttgtgcg agatgccggc gacgacgacg
     2101 tgcatccgct cagcccccgt aggtgtggaa gccgctgagg tacggtaccg cggcgaaccg
     2161 gatcacgagc agcaggacga gccccgcgac ggccagccac gagacggagc ggccggacca
     2221 gtcgccgcgc acgcgcccgg cgatgagcgc gagatacacg gcccaggccg cggccgacag
     2281 cagcaccatc gggtccgtcg gccagccggg gaccttgaag gtctcggcgc ggatgacccc
     2341 ggccgccatg cccatggtga ggaacggcag gccgagcagc gtggcgtggt agatgagccg
     2401 gtcgagcgcg gccagcgagg gcaggcggcc gagcacgccg ccgggcttgc gccgccgcag
     2461 cagcgtctcc tcgatgaggt agatgatcgc cgcgccgccg gcgacgtaga ggcagccgat
     2521 ggccacgagc atcacggcca cgtggacgat gaccacgtcg ctgcgcagcg ccggcacgag
     2581 acccgcgggg gggtcgtacg agaccagcgc gaccgtcagc agcacgagca ccaccggcat
     2641 cacgtacagg cccacggcct tgatcttggt gaacgcctcg aggacgtgcc acacgagcac
     2701 gatcgcccag gccacgagga ccatggactc gtagaggccg gagaacggga agtgcccggc
     2761 gctgacgccg cggaggacga ggcccagggt ctgggccacg agaccggccg cggcgacgac
     2821 gatcccgacc cggttcgtcc acgggcgctg cgtgaagaac ccgatcagga acacggcgaa
     2881 cgcggccgcg tacaggaggt acgcgagcca gatcaggatg accgaggtct gctccatcgg
     2941 ctgggctcag cccgcccggc gccggctgat cggggggcgg gtcactccgc gccggcctcg
     3001 cccgcgggcg ccttcaggcg cttcgcctcg tcctccagca actgcacgtc acgctgcagc
     3061 gtcgccacgc gacgcgcgct catgacgtag tagatcacca gcacggcgag gatgatcgcg
     3121 tacgccgcgg cgacgtactt gacgccttct tcgatggtca tgtggtcagc ctcccagcga
     3181 ggtcttgagg tcggtgacac ggtctctgat cagctcgatc tcgtagtcgc ggcggatgat
     3241 cgccgccatc agcagcagca tgcccaccat gcagatggcg aagctcaccg cgatgctccc
     3301 ctccatgccg ccgccgccgg ggccgacgac caccgggtgg agcccctcgg gcagaaaccg
     3361 cgtggcgaag aacgtgatcg gcacgtcgat gaaggcgatg atcgcgaaca ccgccgcgta
     3421 ggtcgcgcga cgatcctcct cgtcgaccac ggagcgcagc acccagtagg cggcgtagag
     3481 gaagcaggcc agcaggtagg tcgtgagccg cggctcccag gcccaccaca caccccaggc
     3541 cgccttgccc cagatcatgc cggtggccat gacgaggatg ctgaacagca ggccgatgcg
     3601 cacgcccacg tagccgaggc ggtcccactt gaggtcctgc gtgcggaggt aggcgatggc
     3661 ggcgacgaag gcgacgccga acgccacgaa ggccgccgcg gcgataggaa cgtgatagta
     3721 gaagatcttc tggctgaacc ccatgctcga ggcgtctgtc ggggagtacc agaaggccag
     3781 caggagcccg atgatgagaa ggacgctggc ccagatgaac aaagccggtg cgctcaagag
     3841 cttcttcacg ctcactctcc tatggcgtag tcgtaggtac cccaggcggc gacgaggaac
     3901 acggcgtcga aacccaggag aagcaggagc cgagcgacca gactaccgaa actcacgcct
     3961 tctgcgaaga tgagggtggt cgcgccggtc gccgcgatga gcacggggac gatcagcggc
     4021 aggaagagga tcggcagcag caggtcgcgc gagcgcgtgt tcatggagat ggtcgcgagc
     4081 agcgtgccga gggcgcagat gccgaggtcg gcgagcgcca gcacgatgag gaacggcagc
     4141 aggtcgccga ggaagttgcg ctcgatgaag aagagcgcga acacgggcac ggcgaccgcc
     4201 tggatgatca gcaggaagac caggttgccg gccgtcttgg cgaagaagat ggtcacgcgg
     4261 tcgaccgggc agagcagcag gccgtcgagg cagtgctcgt ccttctcctg ggcgaacgag
     4321 cggttgaggc ccagcagcat ggcgaagatg aacgtcaccc acagcatgcc gccggtgaag
     4381 taggtgaggt ccgccccctc cttcacggtg aacgcgtagt ggaagatgat catcgcgagc
     4441 agcacgaaga ggatcatgct gacgagcatg tcccgggtgc gcagctcgcg gatgagatcc
     4501 ttgcgcagga tggccttgaa ctggctccag ccgctcatcc ctacagcacc gacccgtcgt
     4561 ggacgtgctc gcggtagacg gcgctgaacg agtcgtggtc gacgccggcc gcgtgctcgt
     4621 aggcgatgcg gccgccctcg atgatcatca ggtcggtccc ccactccagg cccttggcga
     4681 tgttgtgggt gaccatgacg aacgtgtgct cgtcgcggat ctcggtgagc aggccgtcga
     4741 ggatgtcgac ggcccgcggg tcgagccccg agtgcggctc gtcgagcagc aggacgcgcg
     4801 ggcggtgcag cacggcccgc gcgatcgcca ggcgctggcg catgcccttg ctgaacgtgc
     4861 gcaccacgtc gtagcggcgc gcggagagct cgaggcgctc gagcagctcc gtgatgcgcg
     4921 cctcgcggtc gtcgacgccg tacatgtcgg cgtagaagcg caggttctcc tgcgcggtga
     4981 gatccggata gagcatggcg ttgtgcgaga tgaagccgat ggccttgcgc acaggcatgg
     5041 ggtcctcgcg aacgtcgtgg ccggcgacct tgacgctgcc gccgctcgac gcgagcagcg
     5101 tcgcgagcac gcgcagcgtc gtcgtcttgc cggccccgtt ggggccgaag atgctgagga
     5161 acccgccgcg gggcacgccg aacgagagcc ccttgagcac ctcgcggcgg ccgaaggtct
     5221 tgcgcagccc ctcgacctcg atctcgaggg aaaccgcgcc gtcctgccgg tcgtgttcgt
     5281 ccgacatcgc ggcctccgtc aggcggtggc gccgtccgcc tcggccggtc tcacggcgcg
     5341 ggcctcctgc tcctgcttct gacgcttggg ccacagggag acgacggcgc cggcgcagag
     5401 gaggatcgag ccgatccaca cccagcggat gagcgggaag atgacgatgc gcaacgcgat
     5461 ggtcgtcgag gtgccgtcga acggctcgtc ggccaccacg aagaggtcct cgaacggacg
     5521 gccgaggatc acggcccgca cggccgcgcc gctcacgggg tacacgtcgg tgtgcggccc
     5581 gaccgtaccg agcgcctgcc cgtccctggt cacctcgaag gtcgcctctg cccgcgtcga
     5641 gttctgcgcg ccggtgctct cctccatgcc cttgtacgtg agcgtgtagc cgttgagact
     5701 ggccgtctca cccggctcga ccttgacgat ggtcgactgc tcgaccttgt acacggtgga
     5761 gccgagcagg ccggcgacca cgagcaccat gcccatgtgg acgatgtagg cggcggtgcg
     5821 cgtacggctg cccgtgaagg ccttgccgag gccggaccag aggctgttgc cgggtccggc
     5881 cgcccgccgc gcggaacgca gcacgaactg gatgaccgcc ccgaaggcga agccgcagac
     5941 caggagcccg atgaagcccc agatgctgga ctggaagccc aggtacagcc acagcgggat
     6001 cgaggcgagc atcgtcaccg tcggcagcac cagcgtcttg cgcagctccg cgccctcggt
     6061 cttgcgccag gcgaggagtg gacaaacggc gatcatggcg aggacgacga cgcccagagg
     6121 ctgggccacg gcgtcgtagg tgctcgcccc gaccgtccgc tccatgagga gcggcacggc
     6181 gaccgtggcg aaggcgatgg cgacggcgaa cagcgtgagc agcagattgg tgacgtagta
     6241 gaggaagtcg cgggagagcc agctctccac gtcgtcgcgg ctctcgaacc ggttccagcg
     6301 ccagacgatg agcgccgcgc tgccgaccgc cgtcacgacc aggagggtcg agaggatcca
     6361 gatgagcacc gggttgcggc cgaacgcgtg caccgagctg atgaggccgg tgcgcgtcgt
     6421 ccaggtggcg aggatggtga agaagaaggt cgcggcggcc aggccgagcg cccagcgctt
     6481 gaacaggccg cgtgccttgt agagcgtgaa ggcgtggagc agggcggtcg ccgtcagcca
     6541 ggggacgagc gaggtgttct cgaccgggtc ccaggcccag tagccgccga agctgagcac
     6601 gacgtaggcc caccaggcgc cgagcccgat gcccaggctg aggaagagcc agccgaacac
     6661 ggcccacttc tgcgaccgct gcacccagag cttgtcgccg cggccgagca gcagcgtact
     6721 gacggcgaag gcgaacggca ccgcgaggcc gacgtacccg atgaacaggg ccggcgggtg
     6781 cagcaccata cccgggtgga ggagcagcgg gttgaggccg aacggcatgg cgcccgcttc
     6841 ggccgcgagg aacggattgg agccgctgtc gaagatcatg agcgcggcga acacggccga
     6901 gatgacggcg agcacggcga cggcgccgcc ggtgagccgc tccagccggt tgaggtcgat
     6961 cacggcgatc acgatgaccg cgatcgccag gcacagcagc cagagcagga acgagcccga
     7021 ctgcccggcc cagaagccgg cgatgcggta gaagacggag agagaggtgt ccgagttctc
     7081 gaccacgtag gcgaagctga agtcgccctt gaggaacgcc tgcagcagca ccgccgaagc
     7141 gatgaccgtg gtcaggaaga agccgtagac ggcgtagtag ccgtagcgga tgaggtcctt
     7201 gcgctcagac cagctgccga gggcgagcag ccaggctgcg cccagggcga ggatgagcgt
     7261 ggctacgagt gagtatgtcc cgagatctcg caagcgtcag acctcactgc ccggtcgact
     7321 gggcagcggg tgacgcggac ccttcgtact tgctcgggca cttggtctgg agctggtcgg
     7381 ccgtgatcag cgcgccggtg gaggcgtact tgccgatcac cacgacgtcg acgcccgcct
     7441 cgaaggtgcc gggcatctgg ccgctgtaca gcacgttgac cgtctccgtc ttgccggtga
     7501 ggtcggagat ggtgaagcgc acgccgttcg cgtcgcgctc gatggtgccg tccagcaccc
     7561 tgcccccgac cttg
//
LOCUS       DASUMX010000122         2555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_895902, whole genome shotgun sequence.
ACCESSION   DASUMX010000122 DASUMX010000000
VERSION     DASUMX010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_895902"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            72..1322
                     /gene="pgk"
                     /locus_tag="VFZ86_06085"
     CDS             72..1322
                     /gene="pgk"
                     /locus_tag="VFZ86_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEX5641895.1"
                     /translation="MKYGIHTLDDFAWGGKTVLCRVDINAPLDRETGGLRDITRLKGC
                     APTVSELAEAGARVVLLAHQGGDLEYHNYASTEPHAAVMSELTGRDVGFIDDVCGPAA
                     REAVGRLADGDVLLLDNVRFCGEELTLFERKLELSPEDQAKTLVVRKLAPLADLYVCD
                     AFAAAHRSQPTLVGFEQLLPSAMGRLFEREYENLARLREEPARPCVFVLGGTKIQEAF
                     PMMAAALADGVVDTVLTAGLVANVMLAASGVDLGEPSLEFIRSSQLEPYIEESKAILA
                     AHGDKVVLPADLAVVRGGVRAEVAAAGLPVDQPVVDIGHAAVEEYRRHIAAAGTVFAN
                     GPAGVFEQPETEYGTRAIWEAMADSAAFSALGGGDSVTAMNRFGLAERFDYVCTAGGA
                     MVQFLSGKPMPVVEALKASAVRFG"
     gene            1341..>2555
                     /gene="larA"
                     /locus_tag="VFZ86_06090"
     CDS             1341..>2555
                     /gene="larA"
                     /locus_tag="VFZ86_06090"
                     /inference="COORDINATES: protein motif:HMM:NF033504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HEX5641896.1"
                     /translation="MRLSIPYGDGALEAALPDDARVDVISPGCGLGEEVAPPAAPTAA
                     GEQAEIRRALAQPIGAPRLSELAASARTAAIVVSDVTRPCPSYRFLPALLEELAPLRP
                     EDVSILFALGGHRAHTPEEQRRLVGEEVAGSGARLIDLDAARCVPVGTTSRGTRLEVF
                     APYLEADLRICTGNIEYHYFAGFSGGAKAVVPGLCSYAAIRDNHSMMLAATARAGILD
                     GNPVREDIDEAGALIGVDFIFNVLLDEDKRIIRAVAGHYLEAHRAGAEAYDRRCDLRV
                     PQAADVVIASPGGTPKDINLYQAQKTLDNVAGAVRDGGVIVLVARCREGFGQKTFEEW
                     MTGMGTPQVLIDRIQREFVLGGHKAAAVAGLLARADVYLVSEFPDAVVRSMCMTPFAS
                     VDGAVRAALDRTG"
BASE COUNT          348 a          937 c          927 g          343 t
ORIGIN      
        1 gaccgcctcg aggccgtcgg gctcaccaat cagtacctcg ggctcgcctg acgcggacga
       61 gagggcgcgc catgaagtac ggcatccaca cgctggacga cttcgcgtgg ggcggcaaga
      121 ccgtcctctg ccgcgtggac atcaacgcgc cgctcgacag ggagaccggc gggctgcgcg
      181 acatcacgcg gctcaagggc tgcgcgccca ccgtcagtga gctggcggag gcgggcgccc
      241 gggtcgtgct gctcgcgcac cagggcggcg acctcgagta ccacaactac gcctcgaccg
      301 agccgcatgc ggcggtgatg agcgagctca ccggccgcga cgtgggcttc atcgacgacg
      361 tctgcgggcc ggccgcgcgc gaggcggtcg gccggctcgc ggacggcgac gtcctgctgc
      421 tcgacaacgt gcgcttctgc ggcgaggagc tcacgctgtt cgagcgcaag ctcgagctct
      481 cgcccgagga ccaggcgaag acgctcgtgg tgcgcaagct ggcgccgctc gccgacctct
      541 acgtctgcga cgccttcgcc gccgcgcacc gctcgcagcc cacgctggtc gggttcgagc
      601 agctcctccc gtccgccatg ggccgcctct tcgagcgcga gtacgagaac ctcgcccgcc
      661 tgcgcgagga gccggcgcgg ccgtgcgtct tcgtgctcgg cggcaccaag atccaggagg
      721 cgttcccgat gatggccgcc gccctggcgg acggcgtcgt cgacaccgtg ctcaccgccg
      781 gcctggtcgc caacgtgatg ctggcggcga gcggcgtcga cctcggcgag ccgtccctgg
      841 agttcatcag atcgagccaa ctcgagccgt acatcgagga gtcgaaggcg atcctcgccg
      901 ctcacggcga caaggtcgtc ctgccggcgg acctcgccgt cgtgcgcggc ggcgtgcgcg
      961 ctgaggtcgc cgccgccggc ctgcccgtgg accagccggt cgtcgacatc gggcacgcgg
     1021 ccgtcgagga gtaccgccgg cacatcgccg ccgccggcac cgtgttcgcc aacggcccgg
     1081 ccggggtgtt cgagcagccc gagaccgagt acggcacgcg ggcgatctgg gaggcgatgg
     1141 cggactccgc cgcgttcagc gcgctcggcg gcggcgacag cgtcacggcc atgaaccgct
     1201 tcggcctggc cgagcggttc gactacgtgt gcacggccgg cggcgccatg gtccagttcc
     1261 tctccggcaa gccgatgccg gtcgtcgaag cgctcaaggc gtcggccgtg cgcttcggct
     1321 gacggccggg acggcgcggc gtgagactct ccatcccgta cggcgacggc gcgctcgagg
     1381 cagcgctgcc ggacgatgcc cgggtggacg tcatcagccc cggctgcggc ctgggcgagg
     1441 aggtcgcgcc gcccgcggcc ccgacggcgg ccggcgagca ggccgagatc cggcgtgccc
     1501 tggcgcagcc catcggcgcg ccgcgcctct cggagctggc ggcgtcggcg cgcaccgccg
     1561 cgatcgtggt cagcgacgtc acccggccct gcccgtccta ccgcttcctg ccggccctgc
     1621 tcgaggagct ggcgccgctg cgcccggagg acgtcagcat cctgttcgcc ctcggcggcc
     1681 accgggcgca cacgcccgag gagcagcgcc ggctggtcgg ggaagaggtg gccgggagcg
     1741 gcgcgcgtct gatcgatctg gacgccgcgc gctgcgtgcc ggtcggtacg acctcgcgcg
     1801 gcacgcgcct cgaggtcttc gcgccctatc tcgaggccga cctgcgcatc tgcacgggca
     1861 acatcgagta ccactacttc gccgggttca gcggaggggc caaggccgtc gtccccggcc
     1921 tctgctcgta cgcggccatc cgcgacaacc acagcatgat gctggcggcg acggcgaggg
     1981 ccggcatcct cgacggcaat cccgtgcgcg aggacatcga cgaggccggc gcgctcatcg
     2041 gcgtcgactt catcttcaac gtgctgctcg acgaggacaa gcggatcatc cgcgccgtgg
     2101 ccggccacta cctcgaggcg catcgcgccg gggctgaggc ctacgaccgg cgctgcgacc
     2161 tgcgcgtgcc tcaggcggcc gacgtcgtga tcgcctcacc cggcggcacg cccaaggaca
     2221 tcaacctcta ccaggcgcag aagacgctgg acaacgtggc cggagccgtg cgcgacggcg
     2281 gcgtgatcgt cctcgtggcg cgctgccgcg aagggttcgg gcagaagacg ttcgaggagt
     2341 ggatgacggg aatgggcacg ccgcaggtgc tgatcgaccg catccagcgc gaattcgtgc
     2401 tcggcgggca caaggcggcc gcggtcgcgg ggctcctggc gcgcgccgac gtctacctgg
     2461 tctcggagtt ccccgacgcc gttgtgcgca gcatgtgcat gacgccgttc gcgagcgtcg
     2521 acggcgcggt ccgcgccgcc ctcgaccgca ccggc
//
LOCUS       DASUMX010000123        15829 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_33910, whole genome shotgun sequence.
ACCESSION   DASUMX010000123 DASUMX010000000
VERSION     DASUMX010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 15829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15829
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33910"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="VFZ86_06095"
     CDS             complement(<1..304)
                     /locus_tag="VFZ86_06095"
                     /inference="COORDINATES: protein motif:HMM:NF014126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HEX5641897.1"
                     /translation="MSDISNGRTSAFASLKKMDPVIFGVSAFLTIAFVLWAALDEEGF
                     TAAVDKAFTWITVDWGWLYLVSAFALVVTAVVVMFGRFGTVKLGKPGDKPEFSNFAW"
     gene            complement(332..1729)
                     /locus_tag="VFZ86_06100"
     CDS             complement(332..1729)
                     /locus_tag="VFZ86_06100"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HEX5641898.1"
                     /translation="MYHEVLTEEEVRKIHETSMRVLETVGVEIPVDEAQQVFRDHGFR
                     VENGRVHPTEAQVMSALAGVPKRFVLHARNPERSVEIGGGSAVTAPGYGAPFLVDREL
                     GKRSPTLEDYSNLAKLAHALPNQDVSGHLIVEPEGVDQAHLHMLHAHLIHSDKPFMGS
                     TAGAEGATQTLEMARIAFGEDLGDRAVTVSLVNSLSPLGYANDMLDVLLVYAKARQPV
                     IIAALAMGGTTAPVSLAGMLAMQTAELLAGLVLTQLVSPGTPVLFGSTSSNVDMRLGS
                     LSIGSPETSMVIAAHAQLARFYGIPSRAGGALTDASFPDAQAGFESMLGLKTTIDSGV
                     DYILHSAGILSSYLAFSYEKFVLDDEMCGITRRVRGGFPVDEAALGYDVIAAVGPGGN
                     YLSQPHTLKRCRTEFWKPAVCDRRGLAAWVADGGKDAVDRAAARWQQLVTEHEDPEMD
                     PTARAQLDAYLESHI"
     gene            complement(1969..2619)
                     /locus_tag="VFZ86_06105"
     CDS             complement(1969..2619)
                     /locus_tag="VFZ86_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HEX5641899.1"
                     /translation="MSDELYEQMKQSVIDGEVEDAERLAQEGLDAGIAAGDILDKGFV
                     KGIEEVGDLFGKGEFFLPELVQGAEAMKAAVAVLQPAIDAAGGGRQSLGKAVAGTVAG
                     DIHEIGKTIVCSMLSAAGFTVTDVGCDVPVETFVQKVKDENADLLLLSALLTTTMPNQ
                     QKTIEALKAAGLRDGVKVMIGGAPTTRAWADEIGADGYAEDAIEAVATAKTILGVA"
     gene            complement(2820..3953)
                     /locus_tag="VFZ86_06110"
     CDS             complement(2820..3953)
                     /locus_tag="VFZ86_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018089603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5641900.1"
                     /translation="MKPLRTFRLEEYLGEWEFKVRHHLTASDAQSMTIAELLALGTEG
                     DREGLMQLPLSYIETWGSPALRTAVAGTYERVDADHVLAFAGAEEGLFWTMQELVGPG
                     DHAVVTVPCYQAMETVTLATGADVSALVPRREHGWALDLDELRGLLRPNTKLVAVNYP
                     NNPTGYVPEEATFRELVALCDERGIRLFCDEVYRGIEVDPAQTITQAADLSETAVSLN
                     VASKSYGLPGLRVGWIACRDRALLERLEKRKHYTSICNAGPAEYLAAVALRHRDAIWE
                     RNRGVVAANRPLFDDFFARWSDLFDWRPPDGGCVCFPRYKGGDVEEFCRQLLDAEGVL
                     VLPASMYASEIADVPKDHFRVGIGRLGLEEGLEAFDHFLRAQH"
     gene            complement(3950..4573)
                     /locus_tag="VFZ86_06115"
     CDS             complement(3950..4573)
                     /locus_tag="VFZ86_06115"
                     /inference="COORDINATES: protein motif:HMM:NF019947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX5641901.1"
                     /translation="MPVKTPVPAWLEPHLPTCEAIVALLAPYAEVAVHDIRRARIVAI
                     WNALSKRKVGERSRSDELPPQPEDVRVIGPYPSVLADGRAVTSVGVVLRNAKGAPRGL
                     LWINFDRSPFDAMIDVLVRFAAPVEDRPAELPERDWREQILLVVDEECRSRRLRRERL
                     SRGQRLELVRVLDDRGLFATRNAAAHAAGALGVSRTTVYALLKEARS"
     gene            complement(4641..5594)
                     /locus_tag="VFZ86_06120"
     CDS             complement(4641..5594)
                     /locus_tag="VFZ86_06120"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX5641902.1"
                     /translation="MRALTISELESESEVPRRTIYYYVRLGLLPEAQKASRTRALYTE
                     DHLALLRDIEALRSEGLRPATIRSRLAGRLNAASENGVDLVARRDEETRDSILRAATR
                     RFAQRGYKGTRMADLVAELGITPQLLYQHFATKRDLFVACYRIAVLYMADVIVPRVGP
                     EGDPAVRLVWYMWADEGIKAFAPHMFALAVEASRHDEAARRDMREAYERIFRELIDDL
                     ARLRTSEREPPISDELISHGLMGAFQQMLARSALDDEYSWREVTRHTLGLFLLLLTAY
                     RGEQDLGALLGPYEAFLAEVAELPPPVPPELRPDAAPSEPS"
     gene            5689..6321
                     /locus_tag="VFZ86_06125"
     CDS             5689..6321
                     /locus_tag="VFZ86_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051825.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HEX5641903.1"
                     /translation="MTPTPLAQSVGELQMDEAARLVQEKLDAGDAPLAVLEELQQGMS
                     IVGDRFEAGEYYLSELIFAGELFKQASAPLQDAIAEAAGNAHGIIVLGTVKNDIHEFG
                     KNIAGTVLSSNGIKVVDLGVDVDPQAWVDAVNETGATLVGMSALLTTVFDQMKEAVAT
                     IERAGLRDKVTILIGGGPMTEEAAQYVGADHYCETAQDGVVVAKNVLGVS"
     gene            6323..7471
                     /locus_tag="VFZ86_06130"
     CDS             6323..7471
                     /locus_tag="VFZ86_06130"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HEX5641904.1"
                     /translation="MAETQGPPPGAPQGPPPNPLFLERMNRIQTAIHCGTPDRVPISL
                     VLDTFACNTMGVKYSEFCADLDVAGQTMLAATEKLGDVDSVQWATYAPAILGMLWLAP
                     MKLPGRDLPEDSLWQLDEQVRIQAEDYDRIVEMGWGPWFGQYIGKYLGEAAAAGAALQ
                     QKAPRWVGEFMGRGYVVFSASTVDHPYEHLCGGRTIQEFTMDLFTRPDKVQAAMDVIM
                     AEKREQTRQMVRTIGPVGYWVGSWRTAPQFLSPKLWDRFVWPYMKELVEIVAEEGGIP
                     MLHYDACWDREIERLLELPARTCVLATDGATDLFRAKEILGGHMCLMGDVPPAMLTLG
                     TTDEVKAYCDRLLTEAGPDGFIMAMGCALPPNAKFENVKAMVDSVQGK"
     gene            complement(7551..8009)
                     /locus_tag="VFZ86_06135"
     CDS             complement(7551..8009)
                     /locus_tag="VFZ86_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015948618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX5641905.1"
                     /translation="MTAASVLKERQRDIGSEIFVSDWMRVTQDQIDRFADATGDHLWI
                     HVDTERAADGPFGATIAHGLFILSHLPFFRYRVPLATEAPAMTVHYGFDRVRFAHPVS
                     PGVRFRDRIVLASMEERQGDRLLVKMTHTIEIEAADKPACVADELVLLCF"
     gene            complement(8021..9280)
                     /locus_tag="VFZ86_06140"
     CDS             complement(8021..9280)
                     /locus_tag="VFZ86_06140"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HEX5641906.1"
                     /translation="MSTDIQPGELAAQRLKLFEDAVALRKPERVPLMPLALQYFHTRV
                     AGVSDKDAGYDAQLNFSCLRDATTRFGWDWVPGMAVPPSRSLEALGSKQIRWPGGDLP
                     DDVQFQWVEGEYLKADEYDALLADPTRFTLHTLWPRLASAFGVLGELPLPPAWWMNTM
                     YMPLAWGALLGQPKMTALFEALAEMSRDAGAFMAAFGRFMGEMAGMGYPVAFVGVTKV
                     PFDIVSDDYRGLRGSTTDLFRQPDKLHALMGMLTPMAIGQGIGAFKGSGNPRVFIPLH
                     RGAAGFLSDEQFATYYWPGLKAVIYALIEAGVTPCPFFEGDYTPRLKYLAELPPGKIA
                     AHFDRMDRAKFKEACGDVLTYWGDVPGSLMVTGTPAEVKDYVKRLIDDFGDTGGLIID
                     GANSITEEAKPENVMALREAVDEYGVD"
     gene            complement(9292..9930)
                     /locus_tag="VFZ86_06145"
     CDS             complement(9292..9930)
                     /locus_tag="VFZ86_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008617913.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HEX5641907.1"
                     /translation="MTTPLTDAVLAMDEAGALQIVKDRLDAGEAPEAVLDDAKGAMTI
                     LGDRFACEEVFIPELIMGGEIMKGIATELKPRITADASAETRGTVVIGTVHGDIHDIG
                     KDVVCMMLEVSGYEVHDLGIDVPVEKFVAAIDEFRPEVVGLSGLLTLAFDAMKDTIEG
                     IAAAGKRDQVKVMIGGSPVDEQVCIYTAADGWGRDAAAALRLAAEWTASAAG"
     gene            complement(10054..10998)
                     /locus_tag="VFZ86_06150"
     CDS             complement(10054..10998)
                     /locus_tag="VFZ86_06150"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX5641908.1"
                     /translation="MRALTIAELERESGVPRRTIYYYVRIGLLPAAQKSSPSRALYTD
                     DHLALLREIEALRAEGMRPAAIRSRIAGRIAAAGENGVDLVARREEETRDAILKAATR
                     RFALKGYRGTRMADLVAELGITPQLLYQHFATKRDLFVACYKVAVQYINVLLRDRFAE
                     AQDSPERMLWYMYADEGIKAFAPGMFALALEAAQHDEQARRDLREAYETIFRDHIAEL
                     ASLRTSADAPPLPDELISHGIMGAFEQMLARASLDDRYSWRDVVRTTLGIYLAVLAMY
                     SGELDVGARLEPYEARLSEVAELPPPVPPELAAQTRDA"
     gene            complement(11163..12869)
                     /locus_tag="VFZ86_06155"
     CDS             complement(11163..12869)
                     /locus_tag="VFZ86_06155"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX5641909.1"
                     /translation="MSPSYAQPTTPAETAASSPQIPDAAYRALEDIVGAANVSRDAAV
                     LDSYAFQWLAELVRPDQSHYMPRPAAVVLPGSTEEVQAVVRLANKYGLKVKPHGTGWY
                     HWSGPIKDEDATLQLDMRRMDRILEIDEQNLYAVVEPYVIAARLQADLLPLGLNLNIP
                     GVGCSSSIVASACAYHGAGPDTYFMGGNAENVLGMEWVTPEGEIVRTGSLGSGDGWFC
                     SEGPGPSVRGICRGTLGSRGGLGVYTRCAVKLAPHLGQTEWNVRGTAPAYRLPVHERM
                     RVYTIAVPDWDAWAEIYYRIYDNEIGYIFHRQFNLAGADLAAAFWLMYNDASKTLSDV
                     PGMVADPEIQKLTEELRISFQIILQGRSEDDIELQDKILDAILEEVGGWKVERFCEQD
                     MAEFTNVYLQRLGHKHINFVWAGGYIGSWMQFGTPDWVKGYVPVAKAGLERDAAGGKL
                     VQCGGDAMMGSGSGIPLGGATGLEQFVSYDPDDHDSTVAGIKHMEDAVTDAASIKYPP
                     GKEFLYLQIGWTDEAVWNALAHARQPQVFRYQRMIKDAFDPNDVGDRMYPTLPLEYEA
                     GS"
     gene            complement(12866..14242)
                     /locus_tag="VFZ86_06160"
     CDS             complement(12866..14242)
                     /locus_tag="VFZ86_06160"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEX5641910.1"
                     /translation="MALTDYRPDAMRCTRCSYCKWIPYDLVKSARFAEGCPSIKAGKF
                     HAYSAGGKLVTALSLMDGRSEVTDQVVDIAFKCQLCGNCDVACKLCRYDMEPILALRE
                     LRAHLVGLDRVPESYRPLIERTRAALAGKGPKTPADRNAWAEGLGLVDPAAEPVDVVF
                     YAGCKYSMEESLRETVRTQVQLLQLAGLKVGLFENGCCGGLADKMGYRDEAAEAGARM
                     LKQWAEAGVKTVVTPCADCRQVFTRVYPRLEGAWEGQILHTVECVEQLVRDERLELTT
                     PVPLTVTYHDPCNLGRQGEPYQSWEGEEKKIYGQAVVYDPPRPRHNGAHGVYDAPRDV
                     LAAIPGVELVEMERSREAAWCCGAGGACREAFPEYSAATAAERVEEAAYTGAQALVTA
                     CSRCELNFSEAVAADTASGGPSAGMAVHDVLELVAVSAGLMPAPAGVTAVTAAVTPPA
                     TADGKEAS"
     gene            complement(14244..15773)
                     /locus_tag="VFZ86_06165"
     CDS             complement(14244..15773)
                     /locus_tag="VFZ86_06165"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX5641911.1"
                     /translation="MNEIIDGLAALVGRDNVAADPASLAEYAQDESFVPRLTPWAVVR
                     PGSAEEVQQVVAWANETATPLVPVSSGGPHFHGDTVPSVPGAVMLDLRRMDKILRVDR
                     RNRMVVIEPGVTYPQLQAALAEQGMRVTPPLYPRANKSVVASLLERQPTLIPRYNFHL
                     PEPLRSCGVVWGNGDVMFTGEAGNGPLSLQAQWDAGLVQADPKGPGQTDFFRLLTGAQ
                     GTFGVVTWAAVRCELVPAAHEYLFAAAHELDDLVDLVYRLDRIRLGDEVLVLNGACLG
                     RLLAASGAAVDSAALPTWTLAVGLGGREHFAAERVAVQKKDALALAGEYGVTLTPALP
                     GAATEDVAAVLAGCCDAPWKLAAEGACADVLFVTTLEEAPAFVEIAHIVADGHGYAAE
                     DVGVYIQPQHQGVTHHVELSLPYEAGDAATRAGMQALSDDLAANLMAAGAYFSRPYGS
                     WAQPVYNRDAAARDALRKVKTIFDPAGILNPGKLCFGAPGAAGPGDRAAMPAVATAKE
                     A"
BASE COUNT         2426 a         5364 c         5544 g         2495 t
ORIGIN      
        1 accaggcgaa gttggagaac tccggcttgt cgccgggctt gccgagcttg acggtgccga
       61 agcggccgaa catcacgacg accgcagtga ccacgagagc gaacgcggag accaggtaca
      121 gccaacccca gtcgaccgtt atccaggtga acgccttgtc cacggcggca gtgaaaccct
      181 cttcgtcgag cgccgcccac agcacgaacg cgatggtcag gaaggctgag accccgaaga
      241 tgaccgggtc catcttcttc aggctggcga acgccgaggt ccgcccgttg gagatgtcac
      301 tcatgtcaga ttcctccgct accgtgccgg atcagatgtg cgactcgaga taggcgtcga
      361 gctgggcccg cgctgtgggg tccatctcgg ggtcctcgtg ctctgtgacc agttgctgcc
      421 agcgggcggc ggcccggtcc acggcgtcct tgccgccgtc ggccacccaa gcggccaggc
      481 cacgacggtc gcagaccgcc ggcttccaga actcagtgcg gcaccgcttg agcgtgtgcg
      541 gctgcgagag gtagttgccg ccggggccga ccgcggcgat gacgtcgtag ccgagtgcgg
      601 cctcgtccac cgggaagcct ccgcgcaccc tgcgggtgat gccgcacatc tcatcgtcca
      661 gcacgaactt ctcgtaggag aaggcgaggt acgagctgag gatgccggcc gagtgcagga
      721 tgtagtcgac cccgctgtcg atggttgtct tcagaccaag catcgactcg aagcccgcct
      781 gggcatcggg gaagctggcg tcggtaagcg ctccgccggc gcgcgaggga atgccataga
      841 agcgcgccag ctgtgcgtgg gcggcgatca ccatcgaggt ctccggactg ccgatgctca
      901 gcgagcccag gcgcatgtcc acgttcgagg aagtggatcc gaagaggacc ggcgtgcccg
      961 gactgacgag ctgggtcagc acgagtcccg cgagcaactc ggcggtctgc atggccagca
     1021 tcccggctag cgagaccggc gccgtggtac cgcccatcgc cagcgcggcg atgatgacgg
     1081 gctggcgagc cttggcgtac accagcagca cgtcgagcat gtcgttggcg tagcccaacg
     1141 ggctgaggct gttgacgaga gatacggtga cggcgcggtc gccgagatct tctccgaagg
     1201 cgatgcgcgc catctccagc gtctgcgtag ctccctccgc acccgccgta ctccccatga
     1261 acggcttgtc cgaatggatg agatgggcgt gcagcatgtg gaggtgcgcc tggtcgacgc
     1321 cctccggctc gacgatcagg tggccgctga cgtcctggtt cggcaaagcg tgggccagct
     1381 tcgccaggtt ggagtagtcc tccagtgtcg gcgagcgctt ccccagctca cggtcgacga
     1441 ggaacggcgc accgtagccc ggtgccgtga cggcgctgcc accgccgatc tcgacactgc
     1501 gctctggatt gcgggcgtgc agtacgaagc gcttggggac accggcaagc gccgacatga
     1561 cctgcgcctc cgtgggatgg acacggccgt tctcgacgcg gaatccgtgg tcacggaaca
     1621 cctgctgcgc ctcgtcgacc ggaatctcga ctcccacggt ctcgagcacg cgcatgctcg
     1681 tctcgtggat cttcctgacc tcttcctctg tcaggacttc gtggtacatg gcatcgtccc
     1741 cttggcttca ctgctggccg gtcccgaaac cggtcctggc cctgatatat ccgtaataga
     1801 ttacggatac atcgacacta taggaagact ggggcggcaa tgtcaacgtt attgaggtcg
     1861 cctattcgag gcggacaagt cctgccgcga tccgcaagaa gccgaaaggg cggcggcgca
     1921 tctgcgccgc cgccctttcg gcggtctcac attctgacga gtgctcgctc aggcgacgcc
     1981 caggatggtc ttggcggtgg ccacggcctc gatggcgtcc tcggcgtagc cgtcggcgcc
     2041 gatctcgtcg gcccaggcgc gcgtcgtggg ggcgccgccg atcatgacct tgacgccgtc
     2101 gcgcaggccg gcggccttga gcgcctcgat cgtcttctgc tggttgggca tggtggtggt
     2161 gagcagggcg ctgagcagga gcaggtcggc gttctcgtcc ttgaccttct gcacgaaggt
     2221 ctcgacgggc acgtcgcagc cgacgtcggt gaccgtgaag ccggcggcgg acagcatgct
     2281 gcagacgatg gtcttgccga tctcgtggat gtcgccggcg acggtgccgg cgacggcctt
     2341 gccgaggctc tggcggcctc cgccggcggc gtcgatggcc ggctgcagca cggcgacggc
     2401 ggccttcatg gcctcggcgc cctggaccag ctcggggagg aagaactcgc ccttgccgaa
     2461 gaggtccccg acctcctcga tgcccttcac gaagcccttg tcgaggatgt cgccggcggc
     2521 gatgccggcg tcgagaccct cctgggcgag gcgctcagcg tcctcgacct cgccgtcgat
     2581 gacgctctgc ttcatctgct cgtaaagctc gtccgacatg gttcctccct ttgcacgatc
     2641 tgtccgcctt gtgcgtccgc aagaacgcgt gggttccacc cagggcgcca cgatacgacc
     2701 acgaacgggg cgcgtcaagc aagtaaatcc ggagaccact tgcgccaagt atcaggaccg
     2761 cgggcagggg caacaagtaa cggacagggg tagtccgaaa tgaccccctt gcagcgaatt
     2821 tagtgctgtg ccctcaggaa atggtcgaag gcttcgaggc cttcttcaag gccgagcctg
     2881 ccgattccga cgcgaaagtg atctttcggc acgtcagcga tctccgacgc gtacatgctg
     2941 gccggcagga ccaggacgcc ctcggcgtcc agcagctgcc ggcagaactc ctccacgtcg
     3001 ccgcccttgt accttgggaa gcagacgcag ccgccgtcgg gcggccgcca gtcgaagagg
     3061 tccgaccagc gggcgaagaa gtcgtcgaac agcggccggt tagcggccac gacgccgcgg
     3121 ttgcgctccc agatcgcatc gcgatgacgg agcgcgacgg ccgccaggta ctcggccggg
     3181 ccggcgttgc agatgctggt gtagtgtttg cgcttctcga ggcgctcgag tagcgcccgg
     3241 tcgcggcagg cgatccagcc cacgcgcagc cccggcaggc cgtaagactt ggaggcgacg
     3301 ttcagcgaga cagccgtctc ggaaaggtcg gctgcctgtg tgatcgtctg agccgggtcc
     3361 acctcgatgc cgcggtagac ctcgtcgcag aacagccgga tgccgcgctc gtcgcagaga
     3421 gcgaccagct cgcgaaaggt cgcctcttcc gggacgtacc cggtcggatt gttggggtag
     3481 ttgacggcga cgagcttcgt gttgggccgg agaaggccgc gcaactcgtc gagatccagc
     3541 gcccagccgt gctcccggcg cgggaccagg gcgctgacgt ccgcgccggt cgccaaggtc
     3601 accgtctcca tggcctggta gcacggcacg gtgacgaccg catggtcccc ggggccgacc
     3661 agctcctgca tcgtccagaa gaggccttct tcggcgcccg cgaacgccag cacgtgatcg
     3721 gcgtcgacgc gctcgtaggt gccggcgacc gccgtacgga gcgccggcga gccccacgtc
     3781 tcgatgtacg agagcggcag ctgcatgagg ccctcgcggt cgccctccgt gccgagggcc
     3841 agcagctccg cgatcgtcat gctctgggcg tccgacgcgg tgaggtgatg gcgcaccttg
     3901 aactcccact cgccgaggta ctcctccaga cggaacgtgc gcagcggctt catgagcgtg
     3961 cctccttgag caaggcgtag acggtggtgc gggacacgcc cagtgcaccg gcggcgtgag
     4021 cggcggcgtt gcgcgtggcg aaaaggcccc tgtcgtcgag gacgcggacc aactcgaggc
     4081 gctgaccgcg tgacaggcgc tcccggcgca ggcgccgcga gcggcactcc tcgtccacga
     4141 cgaggaggat ctgctcgcgc cagtcgcgct cgggaagctc tgccggccgg tcctcgacgg
     4201 gcgcggcgaa ccggacgagc acgtcgatca tggcgtcgaa cggggaacgg tcgaagttga
     4261 tccagaggag cccgcgtgga gcgcccttgg cgttgcgcag gacgacgccg accgacgtga
     4321 ccgcgcgccc gtcggccagg acagatgggt acggcccgat cacccgcacg tcctccggct
     4381 gcggcggcag ctcgtcgctg cgggaacgct cgccgacctt ccgcttcgac agggcattcc
     4441 agatggcgac gatgcgtgcg cggcggatgt cgtggaccgc gacctccgcg tagggcgcga
     4501 gcaacgcgac gatcgcctca caagtgggta ggtgcggctc cagccacgcc ggcacaggcg
     4561 tcttgacggg catcaccgaa cgatacgtct acttcggaca ttccgtcaag cgagcggaag
     4621 gcgctctgcg ctctcggcgg tcaggacggc tcactcggcg ctgcgtccgg cctgagctcc
     4681 ggcggcaccg gtggcggcaa ttcggcgacc tcggcaagga aggcctcata cggcccgagc
     4741 agcgctccca gatcctgctc gccgcgatag gcggtcagca gcaacaggaa gagcccaagc
     4801 gtgtgccttg tgacctcgcg ccaggagtat tcgtcgtcga gcgcggaacg ggcgagcatc
     4861 tgctggaacg cgcccatgag gccgtggctg atgagctcgt ccgagatcgg cggctcccgc
     4921 tcggaggtgc gcagccgcgc gaggtcgtcg atcagctcgc ggaagatccg ctcgtaggcc
     4981 tcgcgcatgt cccggcgcgc ggcctcgtcg tgccgggacg cctccaccgc cagcgcgaac
     5041 atgtgcggcg cgaacgcctt gatgccctca tcggcccaca tgtaccagac aaggcgtacc
     5101 gctgggtctc cctccggacc cacgcgggga acgatcacgt ccgccatgta caggaccgcg
     5161 atccggtagc aggccacgaa gaggtcgcgc ttggtggcga aatgctggta gagcagttgc
     5221 ggggtgatgc cgagctcggc cacgaggtcg gccattctcg tgcccttgta cccgcgctgc
     5281 gcgaaacggc gggtcgcggc gcgcaggatc gagtctcgcg tctcctcgtc gcggcgagcg
     5341 acgaggtcca ccccgttctc gctcgctgcg ttcaggcggc cggccagacg cgaccggatc
     5401 gtggccggcc gcagcccctc ggagcgaagc gcttcgatgt cccgcagcag cgcgaggtga
     5461 tcctccgtgt acagagcacg ggtgcgactg gccttctgtg cctccggcag caggccgagg
     5521 cggacgtagt agtagatggt gcgcctcggc acctcactct cgctctcgag ctcactgatg
     5581 gtcagcgcac gcatatggac aatcgtatag ctgatagtat tcttatcaat acttcaagtt
     5641 gtcgtcactc tttcgttcgg ccgaggacac ggagaccagg gaggtctgat gacacccacc
     5701 ccactcgccc agtctgtggg cgaactgcag atggacgagg cggcgcgtct cgttcaagag
     5761 aaactggacg cgggcgacgc cccactcgcc gtcctcgaag agctccagca gggcatgagc
     5821 atcgtcggcg acaggttcga ggccggcgag tactacctct ccgagctcat cttcgccggc
     5881 gagctcttca agcaggcgag cgcgcccctg caggacgcca tcgcggaggc cgccgggaac
     5941 gctcacggca tcatcgttct gggcaccgtc aagaacgaca tccacgagtt cggtaagaac
     6001 atcgccggca cagtgctgtc gtccaacggc atcaaggtcg tggacctcgg tgtcgacgtg
     6061 gatccccagg cctgggtcga cgccgtcaac gagacgggcg ccacgctggt cggcatgtca
     6121 gctttgctca ccaccgtctt cgaccagatg aaggaggccg tcgccaccat cgagcgcgcc
     6181 ggcctgcgcg acaaggtcac gatcctgatc ggcggcggcc ccatgaccga agaggccgcc
     6241 cagtacgtcg gcgccgacca ctactgcgag accgcccagg acggcgtggt cgtcgcgaag
     6301 aacgtcctgg gggtgagctg acatggccga gacgcaggga ccgcctccgg gagcgccgca
     6361 aggaccgccc cccaacccgc tcttcctcga gcggatgaac cggatccaga ccgcgatcca
     6421 ctgcggcacg ccggaccgcg tgccgatctc tctggtcctc gacaccttcg cctgcaacac
     6481 gatgggcgtg aagtactccg agttctgcgc cgacctcgac gtcgccggcc agaccatgct
     6541 cgccgcgacg gagaagctcg gcgacgtcga ctcggtgcag tgggcgacct acgcgccggc
     6601 catcctcggc atgctgtggc tcgcgcccat gaagctcccc ggccgcgacc tcccggagga
     6661 cagcctgtgg cagctcgacg agcaggtgcg catccaggcg gaggactacg accgcatcgt
     6721 cgagatgggc tggggtccat ggttcggcca gtacatcggc aagtacctgg gcgaggcggc
     6781 ggcggccggc gcggcgctgc agcagaaggc gccgcgctgg gtcggcgagt tcatggggcg
     6841 cggctacgtc gtgttcagcg cctcgacggt cgaccacccg tacgagcacc tctgcggcgg
     6901 ccggacgatc caggagttca ccatggacct gttcacgcga ccggacaagg tccaggccgc
     6961 gatggacgtg atcatggccg agaagcgcga gcagacacgg cagatggtcc gcaccatcgg
     7021 gcctgtcggc tactgggtcg gcagctggcg caccgcgccg cagttcctca gccccaagct
     7081 ctgggaccgc ttcgtgtggc cgtacatgaa ggagctggtc gagatagtcg ccgaggaggg
     7141 cggcatcccg atgctgcact acgacgcctg ctgggaccgc gagatcgagc gtctgctgga
     7201 gctgccggcg cgcacctgcg tgctcgccac ggatggggcg acggacctct tccgcgccaa
     7261 ggagatcctg ggcgggcaca tgtgcctgat gggcgacgtg ccgccggcga tgctcacgct
     7321 cggcacgacc gacgaggtca aggcctactg cgaccggctg ctcaccgagg ccggcccgga
     7381 cgggttcatc atggcgatgg gctgcgctct gccgcccaac gccaagttcg agaacgtcaa
     7441 ggcgatggtg gactcggtgc agggcaagta gctcgacggt ccaccaggtg cggacgcgga
     7501 acggggcggc ccggggaaga tcccgaggcc gccccggctg cgccttcgac tcagaagcag
     7561 aggaggacca gctcgtccgc gacgcacgcc ggcttgtcgg ctgcctcgat ctcgatggtg
     7621 tgggtcatct tgacgaggag ccggtcgccc tgcctctctt ccatggaggc gaggacgata
     7681 cggtcccgga accgcacgcc gggagacacc ggatgggcga accggaccct gtcgaagcca
     7741 tagtggaccg tcatggcagg ggcttctgtg gcgagcggga cccgatagcg gaagaagggc
     7801 aggtgggaga ggatgaacaa cccatgggct atggtcgccc cgaaggggcc gtcggccgcc
     7861 ctctccgtgt ccacatggat ccacaggtgg tcgcccgtcg cgtcggcgaa ccgatcgatc
     7921 tgatcctggg tcactcgcat ccagtcggac acgaagatct cgctcccgat gtcgcgctgc
     7981 ctctccttga gtacggatgc cgccgtcacg gccgcgcctc tcagtccacg ccgtactcgt
     8041 ccacggcctc gcgcagcgcc atgacgttct ccggcttggc ctcctcggtg atggagttgg
     8101 cgccgtcgat gatgagccct ccggtgtcgc cgaagtcgtc gatcagccgc ttgacgtagt
     8161 ccttgacctc ggcgggcgtg ccggtgacca tgagcgaccc cggcacgtcg ccccagtagg
     8221 tcagtacgtc gccgcaggcc tccttgaact tcgcgcggtc catgcggtcg aagtgcgccg
     8281 cgatcttccc cggcggcagc tcggccaggt acttgagccg cggcgtgtag tcgccctcga
     8341 agaacgggca cggcgtgacg ccggcctcga tgagcgcgta gatgaccgcc ttgaggcccg
     8401 gccagtagta cgtggcgaac tgctcgtcgc tgaggaagcc ggcggcgccg cggtgcagcg
     8461 ggatgaacac gcgcgggttc ccgctgccct tgaaggcgcc gatcccctgg ccgatcgcca
     8521 tcggcgtcag catccccatc agggcgtgca gcttgtcggg ctgccggaag aggtccgtgg
     8581 tgctgccgcg caggccgcgg tagtcgtcgg agacgatgtc gaacggcacc ttggtcaccc
     8641 cgacgaacgc gaccgggtag cccatcccgg ccatctcgcc catgaagcgg ccgaacgccg
     8701 ccatgaaggc gccggcgtcg cggctcatct cggcgagcgc ctcgaagagg gccgtcatct
     8761 tgggctgccc gaggagcgcg ccccacgcga gtggcatgta catggtgttc atccaccagg
     8821 ccggcggcag cggcagctcg ccgagcaccc cgaaggcgct tgcgaggcgc ggccacaggg
     8881 tgtgcagggt gaaccgcgtc gggtcggcga ggagggcgtc gtactcgtcg gccttgaggt
     8941 actctccctc cacccactgg aactgcacgt cgtccggcag gtcgccgccc ggccagcgga
     9001 tctgcttcga gccgagggcc tcgagcgagc gcgagggggg cacggccatg cccgggaccc
     9061 agtcccagcc gaagcgggtg gtggcgtcgc gcaggcacga gaagttgagc tgtgcgtcgt
     9121 agccggcgtc cttgtctgaa accccggcga cccgggtgtg gaaatactgc agcgccagag
     9181 gcatgagggg gacacgctcc ggcttgcgca gggcgaccgc gtcctcgaac agcttgaggc
     9241 gctgtgcggc gagctcgccg ggctggatgt cggtgctcat ctcgtctccc ctcagccggc
     9301 cgcggatgcg gtccactcgg cggccagcct gagcgcggcg gcggcgtcgc ggccccagcc
     9361 gtcggcggcc gtgtagatgc agacctgctc gtcgaccggc gagccgccga tcatgacctt
     9421 gacctgatcg cgcttgcccg cggcggcgat gccctcgatg gtgtccttca tcgcgtcgaa
     9481 ggcgagcgtg agcaggccgg agaggccgac gacctcgggc ctgaactcgt cgatcgccgc
     9541 gacgaacttc tcgaccggga cgtcgatgcc caggtcgtgc acctcgtagc cggagacctc
     9601 gagcatcatg cagacgacgt ccttgccgat gtcgtggatg tcgccgtgca cggtgccgat
     9661 gaccacggtg ccgcgcgtct ccgccgacgc gtcggcggtg atccgcggct tgagctcggt
     9721 ggcgatgccc ttcatgatct cgccgcccat gatgagctcg gggatgaaga cctcctcgca
     9781 cgcgaaccgg tcgccgagga tggtcatcgc gcccttggcg tcgtcgagga ccgcctccgg
     9841 ggcttcgccc gcgtccaggc ggtccttgac gatctggagg gcgccggcct cgtccatggc
     9901 gaggacggca tcggtgagtg gtgtggtcat gcagcctccc tgcggtcgcc catgctggct
     9961 ccgagccggc tccctgacct gctcgggggc atcaaacgta tagcaaacgt atcgtcattt
    10021 catttgcttg tctagcagtc atggcaacga gattcaggca tcgcgagtct gagcggcgag
    10081 ctcgggcggg acgggcggcg ggagctccgc gacctcggac agccgcgcct cgtagggctc
    10141 cagccgtgcg ccgacgtcga gctcgccgct gtacatggcg agcacggcca ggtagatgcc
    10201 gagcgtggtg cgcaccacgt cgcgccacga gtaccggtcg tcgagcgagg cgcgcgccag
    10261 catctgctcg aaggcgccca tgatgccgtg actgatgagc tcgtccggga gcggcggggc
    10321 gtccgctgag gtgcggaggc tcgcgagctc cgcgatgtgg tcgcggaaga tggtctcgta
    10381 cgcctcgcga aggtcgcggc gcgcctgctc gtcgtgctgc gcggcctcca gggccagcgc
    10441 gaacatgccg ggcgcgaacg ccttgatgcc ctcgtcggcg tacatgtacc agagcatccg
    10501 ctcgggggag tcctgcgcct ccgcgaaccg gtcgcgcagg agcacgttga tgtactgcac
    10561 ggcgaccttg tagcaggcca cgaagaggtc gcgcttggtg gcgaagtgct ggtagaggag
    10621 ctgcggggtg atcccgagct cggcgacgag gtcggccatc cgcgtgccgc ggtatccctt
    10681 gagcgcgaag cggcgggtgg ccgccttgag gatcgcgtcg cgcgtctcct cctcgcggcg
    10741 cgcgacgagg tccacgccgt tctcgccggc cgccgcgatg cggccggcga tccgcgatct
    10801 gatcgccgcc ggccgcatcc cctccgcgcg cagcgcctcg atctcgcgca gcagcgccag
    10861 gtggtcgtcg gtgtagagcg cccggctggg gctggacttc tgcgcagccg gcagcagacc
    10921 gatgcggacg tagtagtaga tcgtccgcct cggcactccg ctctcgcgct cgagctccgc
    10981 gatggtcagc gctcgcacgt cactcctcgg tggatggccc tgccgccttc agaggcgatg
    11041 atgtcacacg agagcgcgac cgtcgccgga ggaggaggta ggggcggccc cgggaggatc
    11101 tcccagggcc gccccgtgag ccgtcggggg gacgacggac ttcggcggtc ggccggctcg
    11161 gatcagctgc cggcctcgta ctcgagcggc agcgtcgggt acatgcggtc gcccacgtcg
    11221 ttcgggtcga acgcatcctt gatcatgcgc tggtagcgga acacctgcgg ctgcctggcg
    11281 tgcgccagcg cgttccagac cgcctcgtcg gtccagccga tctgcaggta caggaactcc
    11341 ttgcccggcg ggtacttgat ggaggcggcg tccgtgacgg cgtcttccat gtgcttgatc
    11401 ccggccaccg tggaatcgtg gtcgtcgggg tcgtagctga cgaactgctc gagtccggtg
    11461 gcgccgccca gcgggatgcc cgagccggaa cccatcatcg cgtcgccgcc gcactgcacg
    11521 agcttgccgc cggcggcgtc gcgctccaga ccggccttgg ccaccgggac gtaccccttg
    11581 acccagtccg gtgtgccgaa ctgcatccag ctgccgatgt agccgccggc ccacacgaag
    11641 ttgatgtgct tgtggccgag tcgctgcaga tagacgttgg tgaactcggc catgtcctgc
    11701 tcgcagaacc gctcgacctt ccagccgccg acctcctcga ggatcgcgtc gaggatcttg
    11761 tcctgcagct cgatgtcgtc ctcgctgcgc ccctggagga tgatctggaa gctgatgcgc
    11821 agctcctcgg tcagcttctg gatctccggg tcggcgacca tgccgggcac gtcggagagt
    11881 gtcttggacg catcgttgta catgagccag aaggcagccg cgaggtcggc gccggccagg
    11941 ttgaactgac ggtggaagat gtagccgatc tcgttgtcgt agatgcggta gtagatctcg
    12001 gcccaggcgt cccagtcggg cacggcgata gtgtagacgc gcatccgctc gtggacgggc
    12061 aaacgatagg ccggagccgt gccgcgcacg ttccactccg tctggccgag gtgcggggcg
    12121 agcttgacgg cgcatctggt gtacacgccg agtccgccgc ggctgccgag cgtgccccgg
    12181 cagatgccgc gcacgctcgg tccggggccc tcgctgcaga accagccgtc gcccgagccg
    12241 agggagccgg tgcggacgat ctcgccctcg ggcgtgaccc actccatgcc gagcacgttc
    12301 tcggcgttgc cgcccatgaa gtaggtgtcc gggccggcgc cgtggtaggc gcaggcgctg
    12361 gccacgatgg agctggagca gccgacgccg gggatgttga ggttgaggcc cagcggcagc
    12421 aggtccgcct gcaggcgggc ggcgatgacg tagggctcga cgaccgcgta caggttctgc
    12481 tcgtcgatct cgaggatgcg gtccatgcgc cgcatgtcga gctgcagggt cgcatcctcg
    12541 tccttgatgg gaccggacca gtggtaccag ccggtcccat gcggcttgac cttgaggccg
    12601 tacttgttgg ccagccggac gacggcctgg acctcttcgg tcgagcccgg cagtacgacc
    12661 gcggcgggac gcggcatgta gtggctctgg tccggccgca ccagctcggc cagccactgg
    12721 aaggcgtagc tatcgagcac ggcggcgtcg cgactgacat tggccgcgcc gacgatgtcc
    12781 tcgagtgccc ggtaggccgc gtcggggatc tgcggcgaac tcgcggccgt ctcggccggc
    12841 gtcgtgggct gcgcgtagct ggggctcatg acgcctcctt cccgtcggcg gtggcaggtg
    12901 gcgtgacggc ggccgtcacg gcggtcacgc cagccggcgc cggcatcagg ccggcggaga
    12961 cggcgacgag ctcgagcacg tcgtgcacgg ccatgcctgc ggacgggccg cccgacgcag
    13021 tgtcggcggc caccgcctcg ctgaagttga gctcgcagcg tgagcaggcc gtgacgagcg
    13081 cctgcgcacc ggtgtacgcg gcctcctcga cgcgctcggc cgccgtagca gccgagtact
    13141 cggggaacgc ctcgcggcag gcgccgccgg cgccgcagca ccaggccgct tcacggctgc
    13201 gttccatctc gacgagctcc acgccgggga tcgcggcgag cacgtcgcgc ggcgcgtcgt
    13261 acacaccgtg cgcgccgttg tgccgcggcc gcggcgggtc gtagaccacg gcttggccgt
    13321 agatcttctt ctcctctccc tcccatgact ggtagggctc gccctgccgg ccgaggttgc
    13381 acgggtcgtg gtaggtgacc gtcaacggca ccggcgtcgt gagctcaagc cgctcgtccc
    13441 tgaccagctg ctcgacgcac tccacggtgt gcaggatctg cccctcccac gcgccctcga
    13501 gcctggggta cacgcgcgtg aacacctggc ggcagtcggc gcacggcgtg accacggtct
    13561 tgacgcccgc ctcggcccac tgcttcagca tccgcgcccc ggcctcggcg gcctcgtccc
    13621 ggtagcccat cttgtcggcg aggccgccac agcagccgtt ctcgaacagg ccgaccttga
    13681 ggccggcgag ctggagcagc tgcacctgcg tgcgcacggt ctctcgcagg ctctcctcca
    13741 tggagtactt gcagccggcg tagaacacga catcgacggg ctcggccgcc gggtcgacga
    13801 gcccgagccc ttcggcccat gcgttgcggt cggccggggt cttgggacct ttgccggcca
    13861 gcgccgcccg cgtccgctcg atcagcggcc ggtacgactc gggcacgcgg tccaacccca
    13921 cgaggtgcgc gcggagctcg cgcagcgcga ggatcggctc catgtcgtag cggcacaact
    13981 tgcaggccac gtcgcagttg ccgcagagct ggcacttgaa ggcgatgtcg acgacctggt
    14041 ccgtgacctc actgcggccg tccatcaggc tgagcgcggt gacgagcttg ccgccggccg
    14101 agtaggcgtg gaacttgccg gccttgatgg acgggcaacc ctccgcgaac cgggcgctct
    14161 tcacgaggtc gtaggggatc cacttgcagt agctgcagcg ggtgcagcgc atcgcgtcgg
    14221 ggcggtagtc ggtgagggcc atgtcacgcc tccttcgcgg tcgcgacggc gggcatggcg
    14281 gcgcggtctc ccgggccggc cgctcccggc gcaccgaagc agagcttgcc ggggttcagg
    14341 atgccggccg gatcgaagat ggtcttgacc ttgcgcagag cgtcccgtgc ggcggcgtcg
    14401 cggttgtaga cgggctgcgc ccacgagccg tagggccggc tgaagtaggc gccggcggcc
    14461 atgagattgg cggcgaggtc gtcgctcagg gcctgcatgc cggccctggt cgcggcgtcg
    14521 ccggcctcgt acggcaggct gagctccacg tggtgcgtca cgccctggtg ctgcggctgg
    14581 atgtacacgc cgacgtcctc ggcggcgtag ccgtggccgt cggcgacgat gtgcgctatc
    14641 tcgacgaagg cgggggcctc ctcgagggtg gtgacgaaga gtacgtcggc gcaggcgccc
    14701 tcggcggcga gcttccacgg cgcgtcgcag cagccggcca gcaccgcggc gacgtcttcc
    14761 gtcgccgcgc cgggcaaggc cggcgtgagc gtcacgccgt actcgccggc cagtgccagc
    14821 gcgtccttct tctggacggc gacgcgctcg gcggcgaagt gctcccggcc gccgagcccg
    14881 acggcgagcg tccaggtggg cagcgcggcc gagtcgacgg ccgcaccgga cgcggccagc
    14941 agccggccca ggcacgcgcc gttcagcacc aggacctcgt cgccgagccg tatgcggtcg
    15001 agcctgtaga caagatcgac caggtcgtcg agttcgtgcg cggcggcgaa caggtactcg
    15061 tgggccgccg ggaccagctc acaacgtacg gccgcccagg tcaccacacc gaaggtgccc
    15121 tgcgccccgg tcagcagccg gaagaagtcc gtctgcccgg gccccttggg gtcggcctgc
    15181 accaggccgg cgtcccactg cgcctgcagc gagagcggcc cgttgccggc ctcgccggtg
    15241 aacatcacgt caccgttgcc ccacacgacg ccgcaactgc ggagcggctc gggcaggtgg
    15301 aagttgtagc gcgggatcag cgtcggctgc cgctcgagca ggctcgcgac cacggacttg
    15361 ttcgcccgcg gatacagcgg cggtgtgacg cgcatgccct gctcggccaa tgccgcctgg
    15421 agctgagggt aggtgacgcc cggctcgatg acgaccatgc gattgcgccg gtcgacccgc
    15481 aggatcttgt ccatccggcg caggtcgagc atcactgcgc cgggcacgct gggcacggtg
    15541 tcgccgtgga agtgcggccc gcccgaactg accggcacca gcggcgtcgc cgtctcgttg
    15601 gcccaggcga cgacctgctg gacctcttcg gctgagcccg ggcggaccac cgcccacggc
    15661 gtcaggcgcg ggacgaagct ctcgtcctgc gcatactcag cgaggctcgc cgggtcggcc
    15721 gcgacgttgt cgcggccgac gagcgcggcg agcccgtcga tgatctcgtt cacggtgctg
    15781 gacctcccgg gaccgtagct gacgcgtcgt cagcgccgcc gtgcggcac
//
LOCUS       DASUMX010000124         8662 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1329767, whole genome shotgun sequence.
ACCESSION   DASUMX010000124 DASUMX010000000
VERSION     DASUMX010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 8662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8662
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1329767"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1273)
                     /locus_tag="VFZ86_06170"
     CDS             complement(<1..1273)
                     /locus_tag="VFZ86_06170"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HEX5641912.1"
                     /translation="MPTSDPREQPRPGRLTYLSEADKVAIYEAALEIIGSVGMRVLHR
                     EALALLRSAGCEVSESDLVKIPRELVEKSRETAPAMIEVYDRAGEHAMSLGRFNSYFG
                     TGSDLMSTYDLETGEHRPSALADVARAARLCDALPNIDFVMSCAHPNDIEPARSYLES
                     YRAMVMNTTKPMVMTSEHAGDLAVMCAIAEALRGGPEELAAKPYFTMYLEPVSPLSHP
                     VESLDKLLFCADHGIPAIYSPAPLAGGTAPITVAGHTCQGVAESLFGLVIHQLRKPGA
                     PFLFGIGPAVLDMATSQSSYNAPEYLMTFLCQIEMARWLDLPNWGYGGTSDSQVVDAQ
                     AGMEIAELALLCLAAGSNLNHDVGYLDFGLTASLELIVIVDEYIAMNRKLFAGVEVTP
                     ETLAVDVVRDVGPGGDYLAHRHTAKNVRKAQW"
     gene            1438..2304
                     /locus_tag="VFZ86_06175"
     CDS             1438..2304
                     /locus_tag="VFZ86_06175"
                     /inference="COORDINATES: protein motif:HMM:NF020897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5641913.1"
                     /translation="MKERTPEDEGTTWGAARVLDVLEFLGRRTSPAPASIIASSCHIP
                     RSSTYGLLNLLRERRFVTYRADERAWSLGSAAFELSADAPLFAHGLAVLRAFATSSRG
                     LSVQQIATGASMSRVAVGRILPYLVESDLLHADPDGAYSLGLELVSLASRVGWVDRLR
                     ITARAHLVRLRDATAETANLIVLDGDHAIYVDQVESSYALRHSGWVGRRVPLAGTATG
                     AAFQDRTTSHVAKDAVETGVTAIACAIDLPDDDAAVGITAPSWRVEEFGIARAQQMVE
                     AVAREIAQRTRA"
     gene            complement(2370..2990)
                     /locus_tag="VFZ86_06180"
     CDS             complement(2370..2990)
                     /locus_tag="VFZ86_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020878039.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HEX5641914.1"
                     /translation="MSITDIRQAVLTGDADGAADLARAELEAGTAPAVIMNEALISAM
                     DEAGARMATGELFIPEVLVASEAMRAAMDVITPHLEVEAAGGRGTVVIGAVEGDVHDI
                     GKSLVALMLDGAGFTVHDLGVDVAIPDFVAAAKEHDADVVAMSSLMVTTMPKMKVVIE
                     ALHAAGLEAKPIVGGAPITQAFADEIGAAGYAPDAGGAVRLVRDLL"
     gene            complement(3051..4493)
                     /locus_tag="VFZ86_06185"
     CDS             complement(3051..4493)
                     /locus_tag="VFZ86_06185"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HEX5641915.1"
                     /translation="MGDTLKLKMLSSEQVGTMRQKCLDLLSTMGMRIDHDGALGMLAE
                     AGADVDRETHVVKFPAELVESAVKSVPAEMVVKGADPRHDLPIPHPDGLFYTSTNVQS
                     MLHHDVETKRFEDNSVARYAEWCQLIEILDQTDICAIQTPMDVPAESAEVHALNIQMQ
                     NTTKPLWMHAFSREAVPFMFELMVARMGSVEALREQSVAIINPGTVSPFIMKAMDAEE
                     IIQACHHGVPISSDGHVMTGFTSPMTVAGSAVQNCAETLAHVVLCQVVKPGHPIMMTQ
                     FNCPGDMATGFSTLATPIANVQRAVGQQLTTEGFGIRVSGCPVMTDTYASDGRAVAEK
                     ALGTLLCALAGIDIAYGVGRLGGASLASPVQLIIDDQVVAVVKEYVRGIQVNDEFLAV
                     DEILAMGIGGSLVQSKHTLKHCRDYVEPSLFRFQLQQQWEAEGEKDLYERAVDEYRRL
                     KSVIKPVDLPDDVRREMDAVVARADKALCP"
     gene            5029..6552
                     /locus_tag="VFZ86_06190"
     CDS             5029..6552
                     /locus_tag="VFZ86_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid ammonia-lyase"
                     /protein_id="HEX5641916.1"
                     /translation="MTITLDGSSLTVEKLVRIARDGEQVELDPAAAERINVCRAMLEE
                     KIAAREIMYGTNTGIGEFSEIVLDDDQVQQFQRYLVYNHAAGIGEPAPIEVVRGAMAA
                     RVNVHAHGNSGNRLEITQTLVELLNKGVTPVMSSKGSVGACGDLAPMAQMALVMMGEG
                     ESFYQGERIPSKEALERAGIPVPGLEARDGLATINGSNVLTAMSALLCHDADRWFRQA
                     EIAAAMSLEALLANLKPYNSKLHELRGFSGAVRSAAAIRACIQDSDLATGKIKTKVQD
                     AYSMRSTPQVIGAAHDALKWARSQVEIELNGVGDNPIWVPEEKLTLTGANFQGTPVSL
                     PMEMVGQAITMVSVLSERRLNRMENTATSQGLPDFLTPNPGFYSGLMLSQYTADMLIT
                     EQRILSAPASTMSIPAAADQEDFVSMGMNTALKNRQILENACGVLGIEFMGAAQALDF
                     REFTPGKGVRTAKAVIRKYVDHLDVDRPLYPDHNRMAELVKSGEILDAVEAEVGPLG"
     gene            6734..8353
                     /gene="pckA"
                     /locus_tag="VFZ86_06195"
     CDS             6734..8353
                     /gene="pckA"
                     /locus_tag="VFZ86_06195"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF006820.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HEX5641917.1"
                     /translation="MDEPAVRAQLAEADITVGPVHRNLPAPELIARSLARGEGIFAAN
                     GALVVTTGERTGRSPADRYIVDEPEAADVAWGGPNQPCTPEFFDRQVRRVSDYLRRRE
                     VYVFDGFIGAEPTYRTPIRVVARASWHGLFAHTLFVRPEPIDLEDFQPAFTVIACGEL
                     PVDARPEWEGNEDAGSGERPSQAFVGICLSRRLIIILGTMYGGEIKKALFTAMNHLLP
                     ARNVLPMHCSATAGQKGDVALYFGLSGTGKTSLSADPTRRLIGDDEHGWSDHGVFNFE
                     GGCYAKVIRLSPVHEPQIFNAIRFGSILENVIIDPVTRAIDWDDDSITENTRATYPVS
                     HIPGAIETGLGGMPRNIFLLACDAYGVLPPISRLTPEMASYHFLSGFTAKVAGTEAGC
                     VEPEPTFSACFGSPFMPLDFVVYADMLAQRLQTSPTQCWLVNTGWSGGGCDAGQRMPI
                     EITRNLLKAALEGSLDGVPMTPHPVFGVDVPSSCPGVPDQLLDARSTWADPADYDLRA
                     ATLARQFAANFEAFAGRVSPEIAAAGPRTAG"
     gene            8434..>8662
                     /locus_tag="VFZ86_06200"
     CDS             8434..>8662
                     /locus_tag="VFZ86_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641918.1"
                     /translation="MAAAALLAACGGQTAAPADQASRGPSPAGSSPTAGAAPRPAVII
                     SDRAGPSWKGRLGETVQIDWYDQRRDEMQSEQ"
BASE COUNT         1366 a         3049 c         2915 g         1332 t
ORIGIN      
        1 gccactgcgc cttgcgcacg ttcttggcgg tgtggcggtg ggcgaggtag tcgccgcccg
       61 gccccacgtc gcggaccacg tcgacggcca gcgtctcggg cgtcacctcg acgccggcga
      121 agagcttgcg gttcatggcg atgtactcgt cgacgatgac gatgagctcg agggacgccg
      181 tgagcccaaa gtccaggtag ccgacgtcgt ggttgagatt ggagccggcg gccagacaca
      241 gcagcgccag ctcggcgatc tccatgccgg cctgcgcatc cacgacctgc gagtccgacg
      301 tgccgccgta gccccagttg ggaaggtcga gccagcgcgc catctcgatc tggcagagga
      361 acgtcatgag gtactcgggc gcgttgtaag agctctgcga ggtcgccatg tccaggaccg
      421 ccgggccgat tccgaagagg aacggcgcgc ccggcttgcg cagctgatgg atcacgaggc
      481 cgaagagcga ctcggccacg ccctgacagg tgtggccggc gacggtgatg ggcgccgtgc
      541 cgccggcgag tggcgccggc gagtagatcg ccgggatgcc gtggtcggcg cagaagagca
      601 gcttgtcgag actctccacc gggtggctca gcgggctgac cggctcgagg tacatggtga
      661 agtacggctt ggcggccagc tcttccggcc cgccgcgcag cgcctcggcg atggcacaca
      721 tcacggcgag gtcgccggcg tgctcggagg tcatgaccat cggcttggtc gtgttcatga
      781 ccatcgccct gtaactctcg aggtaggacc gcgccggctc gatgtcgttg gggtgcgcgc
      841 acgacatgac gaagtcgatg ttgggcagcg cgtcgcagag acgggcggcg cgggcgacgt
      901 ccgccagcgc gctcgggcga tgctcgccgg tctcgaggtc gtacgtgctc atcaggtccg
      961 agcctgtacc gaagtagctg ttgaagcggc cgagggacat ggcgtgctcg ccggcgcggt
     1021 cgtagacctc gatcatcgcc ggcgcagtct cccgtgactt ctccacgagc tcgcgaggga
     1081 tcttgacgag atctgactcg ctcacctcgc agccggccga gcgcagcagc gcgagcgcct
     1141 ctcgatgcag aacgcgcatg ccgacggagc cgatgatctc gagtgcggcc tcgtagatgg
     1201 cgaccttgtc cgcttcgctg agataggtca gccggcccgg gcgcggctgt tcgcgtggat
     1261 ctgaggtggg caccgtggct ccttcgtggt cctgttgaga gtctcgcgca ctccaccctg
     1321 acgccgtccc atcgccacat caagagcgct cggacgatgt ccagagttct gtactcgttt
     1381 cattgtcgtg cggcgcaccg gccgctatgc tggattcaca aggaggtgac ggcgatgatg
     1441 aaggaacgta cacccgaaga cgagggtacg acttggggcg ccgcccgcgt cctcgacgta
     1501 ctcgagttcc tcggtcgacg cacgagtccc gcgcctgcga gcatcatcgc gtcgtcgtgt
     1561 cacatccctc gctcgagcac ctacggcctg ctcaacctgc tcagggaacg gcgcttcgtc
     1621 acctaccgcg ccgacgagcg cgcctggtct ctgggctccg cagcgttcga actcagcgcc
     1681 gacgcaccgc tgttcgcaca cggccttgcc gtgctgcgcg cgttcgcgac ctcctcgcgt
     1741 ggcctcagcg tccagcagat cgccacgggc gccagcatgt ctcgggtcgc cgtcggccgc
     1801 atcctcccgt acctggtcga gagcgacctg ctgcacgccg atccggacgg cgcctactcg
     1861 ctcggcctcg aactggtaag cctggcctct cgcgtgggct gggtcgatcg actgcgcatc
     1921 acggcccgtg cccaccttgt gcggctgagg gacgcgacgg ccgagacagc caatctcatc
     1981 gtgctcgacg gcgaccacgc catctacgtc gaccaggtcg agagctcgta tgcgctacga
     2041 cacagcggct gggtcgggcg ccgggtgcct ctcgcgggca cggcgaccgg cgccgccttt
     2101 caggatcgca cgacctcgca tgtcgccaag gatgcggtcg agaccggcgt cacggccatc
     2161 gcctgcgcca tcgacctccc tgacgacgac gccgcggtgg gcatcaccgc gccgagctgg
     2221 cgcgtcgagg agttcggcat cgcgcgggcc cagcagatgg tcgaggccgt cgcgcgggag
     2281 atcgcgcaac ggacgcgcgc gtagccggag cagacggggg ccggcgggca tccgcccgcc
     2341 ggccccaccg actgcatcgt gcgtgcggcc tagagcaggt cgcggaccag gcgtacggcg
     2401 ccgccggcgt cgggtgcgta cccggcggcc ccgatctcgt cggcgaacgc ctgcgtgata
     2461 ggtgcgccgc cgacgatcgg cttggcctcg agacccgccg cgtgcagagc ctcgatgacg
     2521 accttcatct tcggcatcgt ggtcaccatc agcgacgaca tcgcgacgac gtccgcatcg
     2581 tgttccttcg cggccgcgac gaagtccggg atggcgacgt ccacgccgag gtcgtggacg
     2641 gtgaagccgg cgccgtcgag catgagcgcc acaaggctct tgccgatgtc gtggacgtcg
     2701 ccctcgacgg cgccgatgac gaccgtcccg cggccgccgg ccgcctcgac ctcgagatgc
     2761 ggcgtgatga cgtccatcgc cgccctcatc gcctcggacg cgacgaggac ctcagggatg
     2821 aagagctcgc cggtggccat cctggcgccg gcctcgtcca tcgcgctgat gagcgcctcg
     2881 ttcatgatca cggccggcgc ggtgcccgcc tcgagctcgg cgcgcgcgag atccgcggcg
     2941 ccgtcggcgt caccggtgag cacggcctgc ctgatgtcgg tgatcgacat ggttcctcct
     3001 cgcgtctcag agacgctgac tgtcgtggtg tgtcgtgacc agaacggccg tcacgggcag
     3061 agcgccttgt cggcacgggc cacgacggca tccatctcac ggcgcacgtc gtcgggcagg
     3121 tccaccggct tgatgacgga cttgaggcgg cggtactcgt ccaccgcgcg ctcgtagagg
     3181 tccttctcgc cctcggcctc ccactgctgc tgcagctgga agcggaacag gctcggctcc
     3241 acatagtccc ggcagtgctt gagcgtgtgc ttgctctgca cgagactgcc gccgatcccc
     3301 atcgcaagga tctcgtcaac ggcaaggaac tcgtcgttga cctggatgcc gcgcacgtac
     3361 tccttgacga ccgcgacgac ctggtcgtcg atgatgagct ggacggggct ggcgaggctc
     3421 gcaccgccca gccggcccac gccgtaggcg atgtcgatgc ccgccagggc gcacaggagc
     3481 gtgcccagcg ccttctcggc caccgcgcgg ccgtcgctgg cgtaggtgtc ggtcatgacg
     3541 gggcagccgg acacccggat cccgaagccc tccgtcgtga gctgctgccc gacggcccgc
     3601 tgcacgttgg cgatcggcgt cgcgagcgtg gagaatccgg tcgccatgtc cccggggcag
     3661 ttgaactgcg tcatcatgat gggatgcccg ggcttcacga cctggcacag gaccacgtgg
     3721 gcgagcgttt cggcacagtt ctgcaccgcg ctgccggcga cggtcatcgg gctggtgaac
     3781 ccggtcatga cgtgaccgtc ggacgagatc ggtacaccgt gatgacacgc ctggatgatc
     3841 tcctccgcgt ccatcgcctt catgatgaac ggcgacacgg tcccgggatt gatgatggcg
     3901 acgctctgct cgcgcaaggc ctccacactg cccatgcgcg cgaccatgag ctcgaacatg
     3961 aacggcacgg cctcccggct gaacgcgtgc atccagagag gcttggtcgt gttctgcatc
     4021 tggatgttga gcgcgtggac ctcggcgctc tccgccggca cgtccatcgg cgtctggatg
     4081 gcgcagatgt cggtttggtc gaggatctcg atgagctggc accactccgc gtaccgggcc
     4141 accgagttgt cctcgaagcg cttggtctcg acgtcgtggt gcagcatgct ctgcacgttg
     4201 gtgcttgtgt agaagaggcc gtcggggtga ggaatcggca ggtcatgccg cggatcggca
     4261 cccttgacga ccatctctgc gggcaccgac ttgaccgcgg actccacgag ctcggcgggg
     4321 aacttgacga cgtgcgtctc gcgatcgacg tcggcgcccg cctcggcaag catgccgaga
     4381 gcgccgtcgt ggtcgatccg catgcccatc gtcgaaagga ggtcgaggca cttctggcgc
     4441 atcgtgccca cctgctcgga cgagagcatc ttcagcttca gcgtatctcc catgtgctgt
     4501 ctccttggga tgcgccgcgg gagtccgccc agtgggcgca cccggcagcg cgaacgatgg
     4561 aatggagcgg agcgccggga cgcgatgctc gagaccggcg cggcgatcgg cctacgggcc
     4621 gcgagagtcg aagctggtgc tgtccggcgt gagtacgtac gggggcgcac tgggttccgg
     4681 ggtgaaaccg tggaccttcg gcatgacggc agtctgccgg tcagacatcg gacgatcccc
     4741 ctggcggccg gccggccgcc gcgtacgtcg tgaacgatgc aaccgcaagc gcgcgcatgg
     4801 tacgcccgct cgcgcgagaa ggcaacatgc tggggcacct gtccggcggt ccggacggtt
     4861 cgggtcagcg gttcagcgga ttagcgtccg taatcccgga caccagtcct cgcgtctgtg
     4921 caaccagtcc ggcctcacct acattgcgcg atgagtcagc atcggcggcg cccacgaccc
     4981 ttcgagtcgc caggggcacg cctgatcccc gcgagaggag acacaccggt gaccatcacc
     5041 ctggacggct cgtcgctcac cgtcgagaag ctcgtacgga tcgcccgcga cggcgagcag
     5101 gtggagctcg acccggccgc cgccgagcgc atcaacgtct gccgcgcgat gctcgaggag
     5161 aagatcgccg cgcgcgagat catgtacggc acgaacaccg gcatcggcga gttcagcgag
     5221 atcgtgctcg acgacgacca ggtgcagcag ttccagcgct acctcgtcta caaccacgcc
     5281 gccggcatcg gcgaacccgc gccgatcgag gtcgtgcgcg gagcgatggc ggcgcgcgtc
     5341 aacgtgcacg cgcacggcaa ctccggcaac cggctggaga tcacgcagac gctggtcgag
     5401 ctcctgaaca agggcgtcac gccggtcatg agctccaagg ggtctgtggg cgcctgcggc
     5461 gacctcgccc caatggccca gatggcactc gtcatgatgg gcgagggcga gtccttctac
     5521 cagggtgagc gcatcccgag caaagaggcg ctggagcgcg ccggcatccc cgttccgggg
     5581 ctggaggcgc gcgacggtct ggccaccatc aacggctcca acgtgctcac tgcgatgagc
     5641 gccctgttgt gccacgacgc cgaccgctgg ttcaggcagg ctgagatcgc cgcggcgatg
     5701 agcctcgagg cgctgctcgc caatctcaag ccgtacaaca gcaagctgca cgagctgcgc
     5761 ggcttctccg gcgcggtgcg cagcgccgcg gccatccgcg cctgcatcca ggacagcgac
     5821 ctggccacag gcaagatcaa gaccaaggtg caggacgcgt actccatgcg ctccacgccg
     5881 caggtcatcg gcgccgcgca cgacgcgctc aagtgggcga ggagccaggt ggagatcgag
     5941 ctcaacggcg tcggcgacaa cccgatctgg gtgcccgagg agaagctgac gctgaccggc
     6001 gccaacttcc agggcacgcc tgtcagcctg ccgatggaga tggtcggcca ggccatcacc
     6061 atggtcagcg tgctcagcga gcgccgtctg aaccgcatgg agaacacggc gaccagccag
     6121 ggcctgcccg acttcctcac gcccaacccc ggcttctaca gcggcctgat gctcagccag
     6181 tacaccgccg acatgctcat cacggagcag cgcatcctct cggcgccggc gagcaccatg
     6241 agcatcccgg cggcagccga ccaggaggac ttcgtgtcca tgggcatgaa cacggccctc
     6301 aagaaccggc agatcctcga gaacgcctgc ggtgtgctcg gcatcgagtt catgggtgcg
     6361 gcgcaggcgc tcgacttccg cgagttcacg ccgggcaagg gcgtgcgcac cgccaaggcg
     6421 gtgatccgca aatacgtcga ccacctcgac gtggaccgcc cgctctatcc cgaccacaac
     6481 cggatggccg agctcgtgaa gtccggggag atcctggacg cggtcgaggc ggaggtcggc
     6541 ccgctgggct gacagccacg acctgagtac ggcccgggtc tgaccagaca ccgggcgctt
     6601 cagcgaaggc ggcggtccgg ctctccggac cgccgccttc ctgcgtcgtg gcgcatgtgc
     6661 gctgctcatc tgcccgtccc gtgccctgcg cccgcgcgag gcgatacact tggcgccggc
     6721 gaacggagga ccaatggacg aacccgcggt acgcgcgcag ctcgccgagg ccgacatcac
     6781 ggtcggcccg gtccacagga acctgccggc gccggaactc atcgcccggt cgctggcgcg
     6841 cggcgagggc atcttcgccg ccaacggcgc tctcgtcgtg acgaccggcg agcgcaccgg
     6901 ccgctcgccc gccgaccgct acatcgtcga cgagccggag gccgcagacg tcgcgtgggg
     6961 cgggccgaac cagccatgca cgcccgagtt cttcgaccgc caggtacgcc gcgtcagcga
     7021 ctacctgcgc cgtcgcgagg tctacgtgtt cgacggcttc atcggcgccg agcccacgta
     7081 ccgcacgccc atccgcgtcg tcgcccgcgc gtcctggcac gggctcttcg cgcacacgct
     7141 gttcgtgcgc cccgagccca tcgacctcga ggacttccag ccggcgttca cggtcatcgc
     7201 gtgcggggag ctgccggtcg acgcgcggcc ggagtgggag ggcaacgagg acgccggctc
     7261 cggcgaacgg ccgtcgcagg cattcgtcgg catctgcctc agccggcgcc tcatcatcat
     7321 cctcggcacc atgtacggcg gcgagatcaa gaaggctctc ttcacggcga tgaaccacct
     7381 cctgccggca cgcaacgtgc tgcccatgca ctgctcggcc accgcgggcc agaaggggga
     7441 tgtcgcactc tacttcggcc tctccggcac cggcaagacc tcactatcgg ccgatcccac
     7501 tcgccgcctg atcggcgacg acgagcacgg ctggagcgac cacggcgtct tcaacttcga
     7561 gggcggatgc tacgccaagg tcatccgcct ctcgcccgtg cacgaaccgc agatcttcaa
     7621 cgcgatccgc ttcggctcga tcctcgagaa cgtgatcatc gatccggtca cgcgagccat
     7681 cgactgggac gacgactcga tcaccgagaa cacccgcgcc acctaccccg tcagccacat
     7741 accgggggcg atcgagacgg gcctgggcgg catgccgcgc aacatcttcc tgctcgcgtg
     7801 cgacgcctac ggcgtgctgc cgccgatctc gcggctgacc ccggagatgg cgagctacca
     7861 cttcctctcg ggcttcaccg ccaaagtcgc gggcaccgag gctggatgtg tcgaacccga
     7921 gcccacgttc tcggcctgct tcggctcccc gttcatgcca ctcgacttcg tcgtctacgc
     7981 ggacatgctc gcccagcggc tacagaccag cccgacgcag tgctggctgg tcaacaccgg
     8041 ctggtcgggc ggcggctgcg acgccggcca gcgcatgccc atcgagatca cgcgcaacct
     8101 gctgaaggca gcgctcgagg ggtcgctgga cggcgtcccg atgaccccgc atcccgtctt
     8161 cggcgtggac gtgccgtcct cgtgcccggg cgtccccgac cagctcctcg acgcccggtc
     8221 cacctgggcc gacccggcgg actacgacct gcgggcggcc acgctcgcga ggcagttcgc
     8281 cgccaacttc gaggcgttcg ccggccgcgt gtcccctgag atagcggcag ccggcccacg
     8341 gacggccggc tgacctcagc gatggcgccc acggcccgcc tgaccgcgct cgctcgcggg
     8401 gctgcggcgt ccggatgcac gctcgcgctc acagtggcgg ccgccgccct tctggcggcc
     8461 tgcggcggcc agaccgcggc gcccgccgac caggcatccc gcggtccgtc gcccgccggg
     8521 tcgtcgccca ccgccggtgc cgcccctcgc cccgcagtga tcatctcgga ccgcgccggg
     8581 ccgtcgtgga agggccggct tggcgaaacg gtccagatcg actggtacga ccagcgcaga
     8641 gacgagatgc aaagcgaaca ga
//
LOCUS       DASUMX010000125         6170 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_899344, whole genome shotgun sequence.
ACCESSION   DASUMX010000125 DASUMX010000000
VERSION     DASUMX010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 6170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6170
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_899344"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..962)
                     /locus_tag="VFZ86_06205"
     CDS             complement(<1..962)
                     /locus_tag="VFZ86_06205"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEX5641919.1"
                     /translation="MSEEIITPAPDNEGRNVSVTERPGGGYNVKVCAGTACLFAGSMA
                     VFDTFVQEVQDAGLGDSVEVSIIGCHGLCSMSPVVVLSDDTLYGHLKPRDVSKVVEEH
                     LKGGQPVERFLYKDEQSGERIRDWHDIGFYKAQTRIALRDVGVINPETIDAYLAVGGY
                     EAARTALTEKTPEWIIEEVTDSGIRGRGGAGFPTGVKWKFANQSKSDEKYLICNADEG
                     DPGAFMDCSILEGDPHAVIEGMIIGSYAIGAHEGYVYVRAEYPMAVKRLTKAVAEAEE
                     RGFLGPDIFGTGWEFNLHLKLGAGAFVCGEETALIASIEGKRGMP"
     gene            complement(974..1735)
                     /gene="nuoE"
                     /locus_tag="VFZ86_06210"
     CDS             complement(974..1735)
                     /gene="nuoE"
                     /locus_tag="VFZ86_06210"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005722.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HEX5641920.1"
                     /translation="MLGMFKAMRQTLSHLPKKKITVQYPEQREVLPARSRGLFRVVID
                     STSGEARCRSCTLCESNCPVQVIRVNHTSKYRLPVPNAARISEARIAAQQGVDVKHLQ
                     PVLDDYYEHGTGLIAMLQNAQEVYGYLPRLALQEISLQTGVSLSQIYGVSSFYNQFRL
                     SPVGEYIIDVCHGTACHVAGAVQITEALEEELGVADGKTTSDMKFTLSSVACMGACSQ
                     APVMRIGDQTYGGLTPDQTRKIIRDLMEEVGGQKG"
     gene            complement(1735..2856)
                     /gene="nuoH"
                     /locus_tag="VFZ86_06215"
     CDS             complement(1735..2856)
                     /gene="nuoH"
                     /locus_tag="VFZ86_06215"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEX5641921.1"
                     /translation="MVDIIVGILRAILFVVIAYAVVFVAALVNILFERRALAFMQDRL
                     GPNRTGWQGVLQSIADAFKMMGKEDFRPMLADPVLFTLAPVTVMIGAVAIQLVLPYTN
                     GLMAIDLNVGVIFLVAITSFTTLSILMGGWASRNKYSLVGALRAAAQMISYEIPMILG
                     LLIVAMIVGSLRVEAVVAFQAGYEWIVFYAPFTFLIFYIASIAELNRGPFDLPESESE
                     LVSGYGTEYSGMRFGMFFLNEYAGMTIMSMVAVTLFFGGWMGPFAGAIEFWGVDWIGI
                     VWFLVKTYALVFALVWIRLSLPRLQVDQLMAFAWKVLIPLGLVNILVTAAVILWVPSW
                     KWGLAVVGWVSFLAFVALLDPLLKRRLRKQRERQPVVSA"
     gene            complement(2857..4071)
                     /locus_tag="VFZ86_06220"
     CDS             complement(2857..4071)
                     /locus_tag="VFZ86_06220"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017756591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEX5641922.1"
                     /translation="MYDHFETPEPDEAVKLALRRIEEATARGEGSDVDGLAFNEVVVN
                     MGPQHPSTHGVLRLVVTLDGELVKDVIPVIGYLHRSKEKLAERRTYFQYIPIVDRTEY
                     VASINCEWAYVMTVEKLAGMQVTRRAEFIRVITGELMRIASHLVAVGTFTADLSPLGT
                     AMVFYMFREREIILDLMEELTGARMMFNYFRFGGVRYDLPDGWVERCRDFVKMFPAKL
                     KEYEALVDDNAIFLQRTQGKGVITQQDVVDYGITGPNARASGVALDLRRSRPYSVYPE
                     LDFEVVTEQHGDVFDRYRVRMDEMRESLKIIEQCLDMLPDGPVQLGSLRAPYVITPPP
                     GSAYVGQENPRGEYGTYIESDGSRYPYRLKFRDPCFCNLQLFPKLLSGNKIADAVAIS
                     GSIDLVLGGIDR"
     gene            complement(4064..4558)
                     /locus_tag="VFZ86_06225"
     CDS             complement(4064..4558)
                     /locus_tag="VFZ86_06225"
                     /inference="COORDINATES: protein motif:HMM:TIGR01961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEX5641923.1"
                     /translation="MGLVVGSNPLSMDAVAALLVERFGADLEDVRIQEPDTVVARCAG
                     ERLKDVAARLKADADAGYETLNWIAGVDRRTSFEAVYHVYSWKTNTYLELHVELPRAR
                     PEVDSVTGVWPAADWHEREAWDMVGIVFSGHPDLRRILLKDDFVGHPLRKDYVDLAEN
                     HPHV"
     gene            complement(4561..5133)
                     /locus_tag="VFZ86_06230"
     CDS             complement(4561..5133)
                     /locus_tag="VFZ86_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEX5641924.1"
                     /translation="MSERIKTARYPKLEHFSEPNVITTTADFVFSWARSHSVFPFTYG
                     LACCAIEMLTSGFARYDIARYGMEVFRPTPRQCDLMIVAGTVNEKMAPVLKRLYDQMS
                     EPKWVISMGACATSGGPFYDGYNVVNGVDKIVPVDVYIPGCPPRPEALFQGLLELQGK
                     IQQHRFVPEKPAAFVPAPPEGAAPAAAEEA"
     gene            complement(5130..5498)
                     /locus_tag="VFZ86_06235"
     CDS             complement(5130..5498)
                     /locus_tag="VFZ86_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEX5641925.1"
                     /translation="MTNAYLTDWLYVWLFIVAGAALVVLILLFSKLINPSHLTPEKLQ
                     PYECGIPPVGNPWQPFAVRYFVFGLLFLVFDVEAAFLFPWALVFRGLGTAGFIEMLLF
                     IAVLAFGLLYAWRKGALEWA"
     gene            5766..>6170
                     /locus_tag="VFZ86_06240"
     CDS             5766..>6170
                     /locus_tag="VFZ86_06240"
                     /inference="COORDINATES: protein motif:HMM:TIGR01411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEX5641926.1"
                     /translation="MSIGPAEIVIVLILALLVFGPKRLPQMGKSLGKGVREFRKAADT
                     AKQELGLEGVTDQINEVKSTFTEPLKEVTGTFSDLKSSVDVKSAIEAPVAAKGAAAGA
                     AGAALAGTSVPEPPAPAAAQDVTAGVEGYEMPA"
BASE COUNT         1001 a         1990 c         2208 g          971 t
ORIGIN      
        1 ggcatgccgc gcttgccctc gatggaggcg atcagcgccg tctcctcgcc gcagacgaac
       61 gcgccggcgc cgagcttgag gtgcaggttg aactcccagc cggtgccgaa gatgtccgga
      121 ccgaggaagc cgcgctcctc cgcctcggcg acggccttgg tgagccgctt gacggccatc
      181 gggtactcgg cgcgcacgta cacgtagccc tcgtgcgcgc cgatggcgta gctgccgatg
      241 atcatgccct cgatgaccgc gtgcgggtcg ccctcgagga tggagcagtc catgaacgcg
      301 ccggggtcgc cctcgtcggc gttgcagatg aggtacttct cgtcgctctt ggactggttg
      361 gcgaacttcc acttgacgcc ggtggggaac ccggcgccgc cgcgaccgcg gatgcccgag
      421 tcggtgactt cctcgatgat ccactcgggc gtcttctccg tgagcgccgt cctcgcggcc
      481 tcgtaaccgc ccacggccag gtacgcgtcg atggtctcgg ggttgatgac cccgacgtcg
      541 cgcagcgcga tgcgcgtctg cgccttgtag aagccgatgt cgtgccagtc gcggatgcgc
      601 tcgccggact gctcgtcctt gtagaggaag cgctcgacgg gctgaccgcc cttgaggtgc
      661 tcctcgacga ccttgctcac gtctctgggc ttgaggtggc cgtacagcgt gtcgtcgctg
      721 agcaccacga cggggctcat ggagcacagg ccgtggcagc cgatgatgct gacctcgacg
      781 ctgtcgccca gcccggcgtc ctgcacctct tgcacgaagg tgtcgaagac ggccatggag
      841 ccggcgaaca ggcaggcggt gccggcgcag accttgacgt tgtagccgcc gcccggacgc
      901 tccgtgacgc tgacgttgcg gccctcgttg tcgggggccg gcgtgatgat ctcttcagac
      961 atggtgcctc tgctcacccc ttctgaccgc cgacctcttc catgaggtcg cggatgatct
     1021 tgcgtgtctg gtcaggggtg aggccgccgt aggtctggtc gccgatgcgc atgacggggg
     1081 cctgcgagca ggcgcccatg caggcgacgg acgacagggt gaacttcatg tcgctggtcg
     1141 tcttgccgtc ggcgaccccg agctcctcct ccagggcctc ggtgatctgg accgcccccg
     1201 cgacgtggca ggcggtgccg tggcagacgt cgatgatgta ctcgcccacg ggcgacagcc
     1261 ggaactggtt gtagaagctg gagacgccgt agatctggct gagcgagacg cccgtctgga
     1321 ggctgatctc ctggagggcg aggcgcggca ggtagccgta gacctcctgc gcgttctgca
     1381 gcatggcgat gaggccggtg ccgtgctcgt agtagtcgtc gagcacgggc tgcaggtgct
     1441 tgacgtccac gccctgctgg gccgcgatcc gcgcctcgct gatgcgcgcc gcgttgggca
     1501 ccggcaggcg gtacttgctc gtgtggttga cgcggatgac ctgcaccggg cagttgctct
     1561 cgcacagggt gcaggagcgg cagcgggcct cgccgctggt ggagtcgatg accacgcgga
     1621 agaggccgcg gctgcgcgcc ggcagcacct cgcgctgctc cgggtactgc acggtgatct
     1681 tcttcttggg caggtggctg agcgtctgcc gcatggcctt gaacatgccg agcatcaggc
     1741 gctcaccacc ggctggcgct cacgctgctt gcgcaggcgg cgcttgagga gcgggtcgag
     1801 cagcgcgacg aaggccagga acgacaccca gccgacgacc gcgaggcccc acttccagct
     1861 gggcacccac aggatgaccg cggccgtgac caggatgttc accaggccca gcgggatgag
     1921 gaccttccag gcgaacgcca tgagctggtc gacctgcagg cgcggcagcg agagccggat
     1981 ccagaccagc gcgaacacga gcgcgtaggt cttgacgagg aaccagacga tgccgatcca
     2041 gtcgacgccc cagaactcga tggcgccggc gaacgggccc atccagccgc cgaagaacag
     2101 cgtgacggcg accatggaca tgatcgtcat gccggcgtac tcgttgagga agaacatgcc
     2161 gaagcgcatg cccgagtact cggtgccgta gccggagacc agctccgact ccgactcggg
     2221 gaggtcgaag gggccgcggt tcagctcggc gatggaggcg atgtagaaga tgaggaaggt
     2281 gaagggcgcg tagaagacga tccactcgta cccggcctgg aaggcgacca cggcctcgac
     2341 gcgcagcgac cccacgatca tcgccacgat caggaggccg aggatcatgg ggatctcgta
     2401 gctgatcatc tgcgccgcgg cgcgcagggc gccgacgagc gagtacttgt tgcgcgacgc
     2461 ccagccgccc atgaggatgg agagcgtcgt gaagctggtg atggcgacca ggaagatgac
     2521 gccgacgttg aggtcgatcg ccatgaggcc gttggtgtac ggcagcacga gctggatggc
     2581 caccgcgccg atcatgaccg tgaccggcgc gagggtgaac agcaccgggt cggcgagcat
     2641 ggggcggaag tcctccttgc ccatcatctt gaaggcgtcg gcgatgctct gcagcacgcc
     2701 ctgccagccg gtgcggttgg ggccgaggcg gtcctgcatg aacgccaggg cgcggcgctc
     2761 gaagaggatg ttgacgagcg ccgccacgaa caccaccgcg taggcgatga cgacgaagag
     2821 gatggcccgg aggatgccga cgatgatgtc caccatctag cggtcgatcc ctcccagcac
     2881 caggtcgatg ctgcccgaga tggccaccgc gtcggcgatc ttgttgccgc tgaggagctt
     2941 cgggaacagc tgcaggttgc agaagcaggg gtcgcggaac ttgagccggt acgggtaccg
     3001 cgacccgtcg ctctcgatgt acgtcccgta ttcgccgcgc gggttctcct ggccgacgta
     3061 ggccgagccc ggcggaggcg tgatgacgta gggcgcgcgc agcgagccga gctggaccgg
     3121 cccgtcgggc agcatgtcca ggcactgctc gatgatcttg aggctctcgc gcatctcgtc
     3181 catgcgcacg cggtagcggt cgaagacgtc gccgtgctgc tccgtgacga cctcgaagtc
     3241 gagctccggg tagaccgagt acggccgcga gcggcgcagg tcgagcgcga cgcccgaggc
     3301 gcgcgcgttg ggaccggtga tgccgtagtc cacgacgtcc tgctgggtga tgacgccctt
     3361 gccctgggtg cgctgcagga agatcgcgtt gtcgtcgacg agcgcctcgt actccttgag
     3421 cttggccggg aacatcttga cgaagtcgcg gcacctctcg acccagccgt ccggcaggtc
     3481 gtagcgcacc ccgccgaagc ggaagtagtt gaacatcatg cgcgcgccgg tgagctcctc
     3541 catgaggtcg aggatgatct cgcgctcgcg gaacatgtag aagaccatcg cggtgccgag
     3601 cggcgagagg tcggcggtga aggtgccgac cgcgaccagg tggctggcga tgcgcatgag
     3661 ctcgccggtg atgacgcgga tgaactccgc ccgccgcgtg acctgcatgc cggcgagctt
     3721 ctcgaccgtc atgacgtagg cccactcgca gttgatgctg gccacgtatt ccgtgcggtc
     3781 gacgatgggg atgtactgga agtacgtgcg ccgctcggcg agcttctcct tggagcggtg
     3841 caggtagccg atgaccggga tgacgtcctt gacgagctcg ccgtcgaggg tcacgaccag
     3901 gcgcagcacg ccgtgcgtgc tggggtgctg cggccccatg ttgacgacga cctcgttgaa
     3961 cgcgaggccg tccacgtcgg agccctcgcc gcgcgccgtg gcctcctcga tgcggcgcag
     4021 cgcgagcttc acggcctcgt cgggctcggg cgtctcgaag tggtcataca tgcgggtggt
     4081 tctccgcgag gtcgacgtag tctttgcgca gcgggtggcc gacgaagtcg tccttgagca
     4141 ggatgcggcg caggtcggga tggccgctga agacgatccc gaccatgtcc cacgcctcgc
     4201 gctcgtgcca gtcggccgcc ggccagacgc cggtgacgct gtcgacctcc ggcctcgcgc
     4261 gcggcagttc gacgtggagc tcgaggtagg tgttcgtctt ccacgagtac acgtggtaga
     4321 cggcctcgaa cgacgtgcgg cggtcgacgc cggcgatcca gttgagcgtc tcgtaaccgg
     4381 cgtcggcgtc cgccttgagg cgcgcggcga cgtccttgag ccgctcgccc gcgcagcgcg
     4441 cgacgacggt gtccggctcc tggatgcgca cgtcctcgag gtcggcgccg aagcgctcga
     4501 cgaggagcgc cgcgacggcg tccatgctca gcgggttgct gccgacgacg aggcccacgg
     4561 tcaggcctct tccgcggcgg ccggggcagc gccctcgggc ggagccggca cgaacgccgc
     4621 cggcttctcg gggacgaacc ggtgctgctg gatcttgccc tgcagctcca gcaggccctg
     4681 gaacagcgcc tccgggcggg gcgggcagcc ggggatgtac acatcgaccg gcacgatctt
     4741 gtcgacgccg ttgacgacgt tgtagccgtc gtagaacggc ccgccgctgg tggcgcaggc
     4801 gcccatgctg atgacccact tgggctccga catctggtcg tacaggcgct tgagcaccgg
     4861 cgccatcttc tcgttgacgg tgccggcgac gatcatgagg tcgcactggc gcggcgtggg
     4921 gcggaacacc tccatgccgt accgggcgat gtcgtaccgg gcgaagcccg aggtgagcat
     4981 ctcgatggcg cagcaggcga ggccgtaggt gaaggggaag acggagtgcg agcgcgccca
     5041 gctgaagacg aagtcggcgg tggtggtgat gacgttcggt tcgctgaagt gctcgagctt
     5101 ggggtacctg gccgtcttga tgcgctcact catgcccact caagggcccc cttgcgccag
     5161 gcgtacagga gcccgaaggc cagcactgcg atgaacagca gcatctcgat gaaccccgcg
     5221 gtgcccaggc cgcggaagac gagcgcccag ggaaacagga aggccgcctc cacgtcgaag
     5281 acgaggaaga ggagcccgaa gacgaagtag cgcaccgcga acggctgcca ggggttgccg
     5341 accggaggga tgccgcactc gtagggctgc agcttctcgg gcgtgaggtg gctcgggttg
     5401 atgagcttgg agaagagcag gatgaggacg accagcgccg ccccggcgac gatgaacagc
     5461 cagacataga gccagtcggt gagatatgcg ttcgtcattg cgataggagc gcccctcgct
     5521 ccccccctgg gagcccccgg acgcatggcg tactgtacca aagcggcttc ccgagaagca
     5581 aacctgggtg cacaccggtg ggggtatccg cggaccgcgg cgatttgcag ggatttcccc
     5641 cttgcgtacc ccgtccggtc ccgggcgggc ggtcgtccgg tggcggtcgg gaggccgcgt
     5701 tgcacttcgg gctcgcggct cctaccatgg accagcatgg ataccccgac cgaggtgacc
     5761 cccccatgag catcggcccc gccgagatcg tcatcgtcct catcctcgcg ctgctcgtct
     5821 tcgggcccaa gcgcctgccg cagatgggca agtcgctggg caagggcgtg cgcgagttcc
     5881 gcaaggcggc cgacaccgcg aaacaggagc tcgggctgga aggcgtgacg gaccagatca
     5941 acgaggtcaa gtccacgttc accgagcccc tcaaggaagt caccgggacg ttcagcgacc
     6001 tcaagtcgag cgtcgacgtc aagtcggcga tcgaggcacc ggtcgcggcc aagggcgcgg
     6061 cggcaggcgc cgccggcgcc gccctcgcgg gcacgtccgt tcccgagccc ccggcgccgg
     6121 ccgcggcgca ggacgtgact gcgggcgtgg agggctacga gatgccggcg
//
LOCUS       DASUMX010000126        11753 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_469067, whole genome shotgun sequence.
ACCESSION   DASUMX010000126 DASUMX010000000
VERSION     DASUMX010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 11753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11753
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469067"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="VFZ86_06245"
     CDS             <1..598
                     /locus_tag="VFZ86_06245"
                     /inference="COORDINATES: protein motif:HMM:NF037272.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lectin like domain-containing protein"
                     /protein_id="HEX5641927.1"
                     /translation="VAGAGVVDVGGYHTVRLSTPVPVTTGADFVVAVRLRSPGSLTPI
                     PVEHPTRLLSPRPAVARSFISRDGVTWKDLRTRSGFGSSDVCLKAFVDSAGARDEAPP
                     RVRLEPATARPGAGTHVRFTLTDPAFSGGSAVVKLWLREASGTVLRKLRIPAVSVNER
                     ETWRFAAPRRRGDYRIVARAWDVAGHRSALTRAVLQVR"
     gene            complement(1050..2915)
                     /gene="cooS"
                     /locus_tag="VFZ86_06250"
     CDS             complement(1050..2915)
                     /gene="cooS"
                     /locus_tag="VFZ86_06250"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018492 - carbon-monoxide dehydrogenase
                     (acceptor) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HEX5641928.1"
                     /translation="MADSYHESVHTMLKESREAGESTAFDRMDDMGTRCKFCEQGIRC
                     SLCSQGPCRLTEKSPRGVCGIDADGMAMRNFLLQNTMGTATYTYHATEVMKTLAQATP
                     GGTYEIKDWSKLETLAGVVGEAVEPRDTLPKRVADAILTEFNRDHQSPSPFVEKMAPA
                     KRVAKWKELGILPGGIFHESMYATSSCLTNVDGNYVSLALKGLRLGIATAYGAQIPLE
                     LAQDALWGTPTPHPIKVDLGIIDPDYVNIAVNGHEPMIGAALIDAAHAAAAQDKAKAA
                     GAKGLRIVGSIETGQELVQRFEVDDVFVGLTGNWLTEELAIATGGLDVFAADMNCTVP
                     TLGATCAAHGTLLVPVSDLVSIVGAEAPIVFDPANAGDQAKQLIDIAVANFPKRKALG
                     QETPELRTGDAVAGFSTESILGALGGSLDPLLDVIKNGTLKGVCGLVSCTTLRDSDQD
                     SHTVAIAKELIKNDVLVLAMGCGNAGLQVAGLETAEAKRYAGPGLTAVCELLGIPPVL
                     SFGTCTDTGRILLLVGAIADALGVDTCDLPVVATAPEYMEQKATIDAMAALAFGLYTH
                     VNPVPFVTGAPNLVKLVTQDLPGVTGGKLAVETDAKEAVAAMLAWIGAKREALGI"
     gene            complement(2982..3365)
                     /locus_tag="VFZ86_06255"
     CDS             complement(2982..3365)
                     /locus_tag="VFZ86_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003543393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster-binding protein"
                     /protein_id="HEX5641929.1"
                     /translation="MALRSIVKIDEDLCTGCGLCVTPCAEGAIEIIDGKAKVMKEELC
                     DGAGFCLAVCPEGALTVEKREAPEFDEDAAAQKVAEKLAAHVTITQTCMNCGRSEDQA
                     ILFPARTKGESTWVCVKCLPQLIHG"
     gene            complement(3480..4640)
                     /locus_tag="VFZ86_06260"
     CDS             complement(3480..4640)
                     /locus_tag="VFZ86_06260"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEX5641930.1"
                     /translation="MTERMAALRRALEGIMGIPATEGNAVDILRNGDAIFPAMLDAIA
                     GARRTVDFLTFVYWQSDIGARFAAALADSARRGVRVRVLLDAWGAQSIDKPLLEQMDA
                     AGAHVHWFRPLTRMQAHKANHRTHRKVLVVDEEIAFTGGVGIAEEWEGDARNEREWRD
                     THFRIRGPAVDGLRAAFLDNWAETDDVLVEKAVDRFPDQPQPGSTIIQAVRGASTSGW
                     SDISTLVRALLQLAQERVRITTAYFVPDARLNDQLTSAAERGVRVEILLPGPHIDKRF
                     VQLAGQSSYELLLDAGVRIWQFQPAMLHAKIMTVDGVVANIGSANLNARSTRLDDEIN
                     LVALDPDLVATLDDHFDEDLGRSEEIVRGRWGHRPLPQRIMESSARLLRREM"
     gene            complement(4682..5659)
                     /locus_tag="VFZ86_06265"
     CDS             complement(4682..5659)
                     /locus_tag="VFZ86_06265"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX5641931.1"
                     /translation="MSTAALVPETWHLTGDDARQALAHVGRRRLILDGYTRMRYSDGF
                     SHARSLAFLGILLFVEGVIGAIGISRVLGSATFAKAIANALESILPGPAERVLENAAG
                     QANQAASSGYWLAIAFGTIGALVTGASLMGQIERALNRIYGIESDRDTVQKYGRAFLL
                     ALSAGVLSVVALGVLGLGDGFFSSFGAEGATIYWSIARWPLGILLLIAAIALIFRWSP
                     RRRQPAWSWMSFGAIVAVALLVVVTAALNLFFQLSPTFGSTYGPLAGIIALAFWAFGS
                     SIALLVGAALAAQLEAVRAGAGAPRSVAKVVESEPGLRAVAPEGPPPVE"
     gene            complement(5656..6549)
                     /locus_tag="VFZ86_06270"
     CDS             complement(5656..6549)
                     /locus_tag="VFZ86_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014446887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX5641932.1"
                     /translation="MTVIGVLAHSKKELGGSGLDELRRALADKGVGAPAWREVVKPQD
                     VPVAVAELVDAGVETLFVWGGDGTVQQCIDALRSTPPALAILPAGTANLFAGNLGVPT
                     DLEAAVSIGLGGRRRTVDLGTVNGERFGVMAGVGFDAEMIRTAEAGLKERLGRLGYIV
                     AGALGLQRDAVQTRVTVDGTPWFDGPSTCVLVGNMGAVFGGISPFPDASPDDGRLDVG
                     VVTADSVGDWARMLGRTVIGQPEASPFVQTTSAETIEVSLDAALPYELDGDARSETDL
                     LRFRVEPAAIDVCVPAAEEGA"
     gene            complement(6546..7406)
                     /locus_tag="VFZ86_06275"
     CDS             complement(6546..7406)
                     /locus_tag="VFZ86_06275"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX5641933.1"
                     /translation="MDGEATARKRGAPVVSRPRHPADLFLPKPGGLAGAVGPYLGARR
                     PLAAALVIWIVAFVLLGGAIIGLGLLLTRVLLGAGLDAVDAEAIRWFVAERTAAADTL
                     SRWASDVGSTGVILAVSVVTGIVLVLRGHWRQFGFLLFALTLEFSLFLLTVTIVNRDR
                     PDVAQLDAAPPTSSFPSGHTAASWTLYLGLAIVVTSLVRSARVRALVWVAAVALPLLV
                     TGSRMYRGMHFPTDVIAGALLACTALLVALFAVRCTVAATAERDRQAAERERAATEPP
                     PDNPPEEVVS"
     gene            7520..8380
                     /locus_tag="VFZ86_06280"
     CDS             7520..8380
                     /locus_tag="VFZ86_06280"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX5641934.1"
                     /translation="MVELLIAVTVSVAAGVVWGLAVRAFPGADPARSASRGLSGQIAG
                     HGGSRPFLRARLDPGRTTGLALTLALVGLGVVFVAFGVAIAMIRSDSGVVVVDAAVTR
                     WAADRATPFSEAAFGVVTVAGSTVGVIVVALATAVYAVRRWRRWSVALFLLIVVAGQL
                     LLSNLVKIAVERVRPDAPPFNVLTGPSFPSGHSTAAAAVWAAVALVLGSGAPSRTRAA
                     LAGIAAGIAVMVACSRVFLGAHWTSDAIGGLLLGWIWFGLVAVAFGGRVLRLGAPARE
                     APAGERPDSS"
     gene            8483..8644
                     /locus_tag="VFZ86_06285"
     CDS             8483..8644
                     /locus_tag="VFZ86_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019999426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641935.1"
                     /translation="MASCSEMKVGETYVCEECDLEIQVVRSCADSEEGACACAESLRC
                     CGGPLVLKS"
     gene            8902..10875
                     /locus_tag="VFZ86_06290"
     CDS             8902..10875
                     /locus_tag="VFZ86_06290"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX5641936.1"
                     /translation="MALWGVLRLVVFKGQTIPLTYVLPLLVCVWTRSRPMLVAMVAIF
                     AVMAFTETLVFGPAIPDNLLRFVATMVNIAVGALVVYAIMRLRERLEGSLADVTAANV
                     RVQSQSEELAAQNEELVTQADELDALNSEIGQRAKVLAALLEATRRREDRRSVLERVC
                     AACVEVMGEPAVLAAVSTRQGESMLLESAAGRDGAGRPLDAFAYSPASFEESVLQEER
                     VTSLDDAASRPDLSLLREADTSFSSALCAPFRLGGKPGGALCVYGRRAHQWTTEQYDL
                     MGWLADQCGRVIESMRLQESLRESERAQREREEILQSFYQSASFLMGVAELDGDAAVA
                     VSGNRAMEEFFAAAPGEPGSGVVTLDGAPGLEARLAERFRESQARGRPVRFEHVVARG
                     GVTRTLSVTVAPIGTATHGRPLFSFVAEDVSERRRAETALRERELASAAQEERSRLAR
                     DLHDSVTQALFAASLKAEALTGAGDLVSPQVVTTVEEVRRLSRGALAQMRTMLLELRG
                     DPLEEVPIDQLLRNVVEATESRTSTAVALTMSGAAPLPAPLHVALYRISQEALNNVAR
                     HAHAAHAWVHLELAPGRARLSVSDDGEGFTDGPVAPTHLGLRSMRERAEEAGAAFYLA
                     GRPGGGTLVTVEWRGGEAEAGCEDGDGADGEGC"
     gene            complement(10924..11064)
                     /locus_tag="VFZ86_06295"
     CDS             complement(10924..11064)
                     /locus_tag="VFZ86_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrophobic protein"
                     /protein_id="HEX5641937.1"
                     /translation="MLLWILLLILVIVIFGLGFVVKWLFYVAIALFIIWLIMLLVNRS
                     RA"
     gene            11345..11731
                     /locus_tag="VFZ86_06300"
     CDS             11345..11731
                     /locus_tag="VFZ86_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641938.1"
                     /translation="MSTPVTSSLNPWGPISQSHRVRIYQHGRTCEHPDCTTVLSVYNP
                     STYCNAHAALAARMQRRGLASPTRDVACEHCGEVFQTANRARRYCSDRCRMAAFARRK
                     RAAERAKARHREQEVLTELVVGARVG"
BASE COUNT         1633 a         4113 c         4254 g         1753 t
ORIGIN      
        1 ggtcgccggc gccggcgtcg tggacgtcgg cggctaccac acggtgcggc tctcgacgcc
       61 cgtcccggtg accaccggcg ccgacttcgt ggtcgccgtg cgtctgcgga gccccggctc
      121 cctgacaccg atcccggtcg agcacccgac caggctcctc tcccctcgcc ccgccgtcgc
      181 gaggtccttc atcagtcgcg acggcgtgac gtggaaggac ctgcgcaccc gctccggctt
      241 cggctcgtcc gacgtctgcc tcaaggcgtt cgtggattcg gcgggcgcac gcgacgaggc
      301 tccgccgcgc gtgcgcctcg agcccgcgac ggcgcgtccg ggcgccggca cgcatgtgcg
      361 gttcacgctc acggacccgg cgttctccgg cggcagcgcc gtggtcaagc tgtggctgcg
      421 cgaggcaagt gggaccgtgc tgcgcaagct gcgcatcccc gccgtcagcg tcaacgagcg
      481 cgagacctgg cgcttcgcgg ccccgaggcg gcgcggggac taccgcatcg tcgcccgcgc
      541 ctgggacgtc gcgggccacc ggtccgcgct cacgcgcgca gtgctgcagg tccggtagcc
      601 gcgacgtccg cccgccggcc gcgctcgccc gcccgccgcg tccctccgcc gtccctgccg
      661 cacggcgacg cacatggggt ggaggggcat ggcccagtct gtgcatcgga cgcccggccg
      721 gagcaccgga cgctcgcgca agcaaccgac tgctctcgcg atgatgaccc ctggacagcg
      781 acgtcgacgg gctcggcgcg gagcggagcg gctgccactg tcgtagcggt caacgagcga
      841 gggcggggag tgcctatgcg cactccccgc cctcttccgc accgggctgc ggccagctgg
      901 ccggcttgcc tcgcccggtg ctcgggtcgg gcgtccgccc gacccgtctt cacacccgcg
      961 ctcttgtcag atacccaggc cggcgcgctt ctcgtcgatg gagcggtgct cctgcggcgc
     1021 cccactcaat tgatctgggc gctcagcctt caaatgccca aggcttcgcg ctttgcgcca
     1081 atccacgcca gcatcgcggc gacggcctct ttggcgtcgg tctcgacggc gagcttgccg
     1141 ccggtgacgc ccggcaggtc ctgcgtcacg agcttgacca ggttcggggc gccggtcacg
     1201 aacggcaccg ggttcacgtg cgtgtacagg ccgaaggcca gcgcggccat ggcgtcgatg
     1261 gtggccttct gctccatgta ctccggagcc gtggccacga ccggcaggtc gcaggtgtcc
     1321 acgccgaggg cgtcggcgat ggcgccgacg agcagcagga tgcggccggt gtcggtgcag
     1381 gtgccgaagc tgagcaccgg cgggatgccg agcagctcgc agacggcggt gaggccgggg
     1441 ccggcgtacc tcttcgcctc ggccgtctcg aggccggcga cctggaggcc ggcgttgccg
     1501 cagcccatgg ccagcacgag cacgtcgttc ttgatgagct ccttggcgat ggcgacggtg
     1561 tggctgtcct ggtcggagtc gcgcagggtg gtgcagctca cgaggccgca gacgcccttg
     1621 agcgtgccgt tcttgatgac gtcgagcagc gggtcgaggc tgccgccgag ggcgccgagg
     1681 atgctctcgg tgctgaagcc ggccacggcg tcgccggtgc gcaactcggg cgtctcctgg
     1741 ccgagcgcct tgcgcttcgg gaagttggcg acggcgatgt cgatgagctg cttcgcctgg
     1801 tcgccggcgt tggccgggtc gaagacgatc ggggcctcgg cgccgacgat gctcacgaga
     1861 tcgctgacgg gcacgaggag ggtgccgtgg gcggcgcagg tggcgcccag ggtcggcacg
     1921 gtgcagttca tgtcggcggc gaagacgtcg agcccgccgg tggcgatcgc gagctcttcg
     1981 gtgagccagt tgccggtgag accgacgaag acgtcgtcga cctcgaagcg ctggaccagc
     2041 tcctggccgg tctcgatgga cccgacgatg cgcaggccct tggcgccggc agccttggcc
     2101 ttgtcctggg cggcagccgc gtgggcggcg tcgatcaagg ctgcgccgat catcggctcg
     2161 tggccgttga cggcgatgtt gacgtagtcg gggtcgatga tgccgaggtc gaccttgatc
     2221 gggtgcggcg tcggcgtgcc ccagagcgcg tcctgggcga gctcgagcgg gatctgcgcg
     2281 ccgtaggcgg tggcgatgcc gagacgcagg cccttgaggg cgaggctcac gtagttgccg
     2341 tcgacgttgg tgaggcagct ggaggtggcg tacatgctct cgtggaagat gccgcccggc
     2401 aggatgccga gctccttcca cttggcgacg cgcttggccg gcgccatctt ctccacgaag
     2461 ggcgacgggc tctgatggtc gcggttgaac tcggtgagga tggcgtcggc gacgcgcttc
     2521 ggcagcgtgt cgcgcggctc gacggcctcg ccgacgacgc cggcgagcgt ctcgagcttg
     2581 ctccagtcct tgatctcgta ggtgccgccc ggcgtggcct gagcgagggt cttcatgacc
     2641 tcggtcgcgt ggtaggtgta ggtggcggtg cccatcgtgt tctgcagcag gaagttgcgc
     2701 atcgccatgc cgtcggcgtc gatgccgcag acgccgcgcg gcgacttctc ggtcagccgg
     2761 caggggccct gcgaacagag cgagcagcgg atgccctgct cgcagaactt gcagcgcgtg
     2821 cccatgtcgt ccatgcggtc gaacgccgtg ctctcgccgg cctccctgga ctctttgagc
     2881 atcgtgtgta cggactcgtg gtaactgtct gccatggatc cctcccgggg atggttggag
     2941 ctcgcgccgg tggcgcggtc agggctttgg tggcgtgagg atcagccgtg gatgagctgc
     3001 ggcaggcact tgacgcagac ccaggtgctc tcgcccttgg tgcgggccgg gaacaggatg
     3061 gcctgatcct cgctgcgacc gcagttcatg caggtctgcg tgatggtgac gtgggcggcc
     3121 agcttctcgg cgaccttctg ggccgcggca tcctcgtcga actcgggggc ttcgcgcttc
     3181 tccacggtga gggctccttc cgggcagacg gcgaggcaga agccggcgcc gtcgcacagc
     3241 tcctccttca tgaccttggc cttgccgtcg atgatctcga tcgcgccttc ggcgcacggg
     3301 gtgacgcaga ggccacaccc ggtacagagg tcttcgtcga tcttcacgat gctgcgcagc
     3361 gccattcagt tctcgctttc gcgtggtttt cacgattgtg acagtgacaa ctgtacagcg
     3421 cggacaacgg ccgccccatt gacctcggtc aagaacggcg cggcgcggcg gcgcggaggc
     3481 tacatctcgc ggcgcagcag ccgcgccgag ctctccatga tccgctgggg gaggggccgg
     3541 tgtccccacc ggccccgcac gatctcctcg ctccgcccga ggtcctcgtc gaagtgatcg
     3601 tccagggtcg cgaccaggtc cggatccagg gcgacgaggt tgatctcgtc gtcgagccgg
     3661 gtggagcgcg cgttgaggtt ggcggacccg atgttggcca ccacgccgtc gacggtcatg
     3721 atcttggcgt ggagcatcgc cggctggaac tgccagatgc ggacgccggc gtccagcagc
     3781 aactcgtacg acgactgccc ggccagctgc acgaagcgct tgtcgatgtg ggggcccggc
     3841 agcaggatct ccacgcggac gccgcgctcc gccgcgctcg tcagctggtc gttcagccgg
     3901 gcgtccggca cgaagtaggc cgtcgtgatg cggacccgct cctgggccag ctgcagaagg
     3961 gcgcggacca gcgtcgagat gtcgctccaa ccgctcgtcg aggcgccgcg cacggcctgg
     4021 atgatcgtgg acccgggctg cggctggtcg ggaaagcggt cgaccgcctt ctcgacgagg
     4081 acgtcgtcgg tctccgccca gttgtcgagg aacgccgccc gcaatccgtc gacggccggc
     4141 ccccggatgc ggaagtgggt gtcacgccac tcccgctcgt tgcgcgcgtc gccctcccac
     4201 tcctcggcga tgccgacccc tccggtgaag gcgatctctt cgtccacgac gaggaccttg
     4261 cggtgcgtcc ggtggttcgc cttgtgcgcc tgcatgcgcg tgagcgggcg gaaccagtgg
     4321 acgtgcgccc ccgccgcgtc catctgctcc agcagaggct tgtcgatgga ctgcgcgccc
     4381 caggcgtcga gaagcacgcg cacgcggaca ccgcgccgcg cgctgtcggc gagcgccgcg
     4441 gcgaaccggg cgccgatgtc gctctgccag tagacgaagg tgaggaagtc gacggtccgc
     4501 cgggcgccgg cgatcgcgtc cagcatggcg gggaagatcg cgtccccgtt gcgcaggatg
     4561 tcgaccgcat tgccctcggt ggcggggatg cccatgatgc cttcgagcgc tcgccggagc
     4621 gcggccatgc gctcggtcaa gtcccgggat cctgacgtcg ccggcgtctc catcacgggg
     4681 atcactctac gggcggaggg ccctccggcg caacggcgcg cagccccggc tccgactcga
     4741 cgaccttggc gacgctgcgt ggtgcgcccg cgccggcgcg caccgcctcg agctgggcgg
     4801 cgagggcggc gccgacgagc agggcgatgg agcttccgaa cgcccagaag gccagcgcga
     4861 tgatgccggc gagcggaccg taggtcgagc cgaacgtcgg gctgagctgg aagaagagat
     4921 tgagcgcagc cgtcaccacc acgagcaggg cgacggctac gatcgcgccg aacgacatcc
     4981 acgaccaggc cggctgccgc cggcgcggcg accagcggaa gatcagcgcg atcgcggcga
     5041 tcagcagaag gatgccgagc ggccacctcg cgatgctcca gtagatcgtc gcaccctcgg
     5101 cgccgaacga ggagaagaac ccgtcgccca atcccagcac gccgagggcg acgaccgaca
     5161 gcacgcccgc gctcagggcg aggaggaagg cgcggccgta cttctgcacc gtgtccctgt
     5221 ccgactcgat gccgtagatg cggttcagcg cacgctcgat ctgacccatg aggctcgcgc
     5281 cggtgaccag ggcgccgatc gtgccgaacg cgatcgccag ccagtatccg gacgacgcgg
     5341 cctggttggc ctggccggcg gcgttctcga gcaccctctc cgccggcccg ggcaatatgg
     5401 actcgagggc gttggcgatc gccttggcga aggtcgcgct gcccaggacg cgcgagatgc
     5461 cgatcgcgcc gatgacgccc tccacgaaca ggaggatgcc gaggaaggcc agcgatctcg
     5521 cgtgactgaa gccgtcggag taccgcatcc gcgtgtagcc gtccaggatg agccgcctcc
     5581 gtccgacgtg ggcgagggcc tgcctggcat cgtcacccgt caggtgccag gtctcgggga
     5641 ccagggccgc cgtgctcatg ctccctcctc ggcggccggc acgcagacgt cgatggcggc
     5701 gggctccacg cgaaagcgca ggaggtcggt ctcggaccgc gcgtcgccgt cgagctcgta
     5761 gggcagcgcc gcgtccagcg agacctcgat cgtctccgcc gacgtggtct ggacgaacgg
     5821 cgaggcttcc ggctggccga tcaccgtccg tccgagcatg cgggcccagt cccccacgct
     5881 gtccgccgtc acgaccccga cgtccagcct cccgtcgtcg gggctggcgt ccgggaaggg
     5941 ggagatgccc ccgaacacgg cgcccatgtt gcccacgagc acgcaggtgc tgggtccatc
     6001 gaaccagggg gtcccgtcga ccgtgacgcg cgtctgcacc gcgtcgcgct gcaggccgag
     6061 ggcccccgcg acgatgtagc cgagacgtcc gagccgctcc ttgaggccgg cttcggcggt
     6121 gcggatcatc tcggcgtcga acccgacgcc ggccatcacc ccgaaccgct ctccgttcac
     6181 cgttcccagg tcgacagtcc ggcggcgtcc gccgagcccg atgctcacgg ccgcctcgag
     6241 atcggtgggc acgccgaggt tgccggcaaa gaggttcgcg gtgccggcgg ggaggatcgc
     6301 cagcgccggc ggtgtggacc ggagggcgtc gatgcactgc tgtacggtgc cgtcgccgcc
     6361 ccagacgaac agcgtctcga cgcccgcatc gaccaactcg gcgacggcga cggggacgtc
     6421 ctgcggcttg acgacctcgc gccacgcggg tgcgcctacg cccttgtccg caagcgcgcg
     6481 gcgcaactcg tcgaggccgc tgccgccgag ctccttcttg gagtgcgcga gcacgccgat
     6541 gacggtcatg agacgacctc ctctggaggg ttgtcgggtg gaggctcggt cgcggcgcgt
     6601 tcgcgctcgg cggcctgacg gtcacgctcg gccgtggccg ccaccgtgca gcgcacggcg
     6661 aacagggcga cgagcagggc agtgcacgcc agcagcgcgc ccgcgatcac gtcggtcggg
     6721 aagtgcatgc cgcggtacat ccgcgagccg gtgacgagca ggggcagcgc gacggcggcc
     6781 acccagacca gagcgcgcac ccgagcgctg cggacgagcg aggtgaccac gatcgcgagc
     6841 ccgagataca gcgtccagga ggcggccgtg tgccccgagg ggaagctgga ggtcggcggg
     6901 gccgcgtcca gctgtgccac atcgggacga tccctgttga cgatcgtcac cgtgaggagg
     6961 aacaggctga actcgagcgt gagcgcgaag agcagaaagc cgaactgccg ccagtggccc
     7021 cgcaggacga gcacgatgcc cgtcaccacc gagaccgcca ggatgacgcc cgtcgagccg
     7081 acgtccgagg cccaccgcga cagggtgtcc gccgcggcag tacgctcggc gacgaaccag
     7141 cggatcgcct ccgcgtccac ggcgtccagc ccggcgccca gcagtacgcg cgtcagcagg
     7201 aggcccagcc cgatgatcgc gccccccagc aggacgaacg ccacgatcca gatgacgagc
     7261 gcggcggcca gcggccgccg cgcccccagg tacgggccca cggctccggc aagaccgccg
     7321 ggcttgggga ggaagaggtc ggcgggatgg cgagggcgcg aaacgacggg agctccccgc
     7381 ttgcgtgccg tcgcctctcc gtccatgccg cccacctcct cgactggcct cgggtttgcc
     7441 cgggagcggg ccgggtaaac ggcccgtcgt tgacgccaag gacatcccac actcccgggt
     7501 cggaggccgg atcagcctca tggtcgagct cctcatcgca gtcacggtga gcgtcgccgc
     7561 cggcgtggtc tgggggctcg ccgtgcgggc gttcccgggg gccgatccgg cccgctcggc
     7621 gtcgcggggg ctcagcgggc agatcgccgg gcatggcggg tcgcgaccct tcctgcgcgc
     7681 ccggctcgac cccgggagga cgacaggact ggccctcact ttggcgctcg tcggcctcgg
     7741 tgtcgtcttc gtcgccttcg gggtcgcgat cgcgatgatc cgttcggact ccggcgtggt
     7801 cgtcgtggac gctgccgtca cgcgctgggc cgccgaccgg gcgacgccgt tctccgaggc
     7861 cgccttcggc gtcgtcaccg tggcgggcag cacggtcggc gtgatcgtcg tcgccctggc
     7921 gaccgccgtc tatgccgtgc ggcgctggcg gcggtggagc gtggcgctct tcctgctcat
     7981 cgtggtcgcc ggccagctgc tgctctccaa cctggtcaag atcgccgtgg aacgtgttcg
     8041 tcctgacgcc cccccgttca acgtcctcac gggcccgtcg ttccccagcg gccacagcac
     8101 ggcggcggcc gcggtctggg ccgccgtggc gctcgtgctc ggcagcggag cgccatcccg
     8161 cacgcgagcc gccctcgcgg gcatcgcggc cggcatcgcg gtcatggtcg cgtgctcgcg
     8221 cgtgtttctc ggggcccact ggacgtccga cgcgatcgga ggcctgctgc tgggatggat
     8281 ctggttcggc ctggtcgcgg tggcgttcgg cggccgtgta ctgcggctgg gggcgccggc
     8341 acgagaagcg cccgccggcg agcggccgga ctcctcctga cgtccgcggc cgcagtcaac
     8401 gcctgccggt tatcgaccgg gccgccggcg gtatgcgaaa cgtgtccggc ccgaccgtgc
     8461 gccgcggcgg aaggagagcg tgatggcatc gtgcagtgag atgaaggtgg gcgagaccta
     8521 cgtctgcgag gagtgcgacc tggagatcca ggtcgtccgt tcgtgcgcgg acagcgagga
     8581 aggcgcgtgt gcgtgcgccg agtccctccg gtgttgcggg ggtccgctcg tcctcaagtc
     8641 ctgagagctc gtccccccgg gagcccgccc ggccatgtcc gcctgcgcgt gtggccgggc
     8701 gcccgcgccg tacgcgcggc aacagggacg gacgcttcgc cctgaaccca gcttctctac
     8761 tggccgggac ggacgacgag gaggaggcgg catgggagct cgatcgacaa agggtctctc
     8821 tcagaagccg tggaccgttt gagccgggcg accgttgcac ggtcgtcccc tcttccggga
     8881 tggctgctgg ccgcgagtct cgtggcgctg tggggagtcc tccgtctcgt cgtcttcaag
     8941 ggccagacga tccctctcac ctacgtgctg ccgctgctcg tctgcgtgtg gacgcgcagc
     9001 cgcccgatgc tcgtcgcgat ggtggcgatc ttcgccgtca tggccttcac cgagacgctg
     9061 gtcttcgggc ccgcgatccc tgacaacctg ctgcgtttcg tcgccaccat ggtcaacatc
     9121 gcggtcggcg cgctggtggt gtacgccatc atgcggctgc gggagcggct cgagggctcg
     9181 ctcgcggacg tcaccgccgc caacgtgcgc gtccagtcgc agagcgaaga gctcgccgcc
     9241 cagaacgagg agctggtcac acaggcggat gaactggacg cgctcaacag tgagatcggc
     9301 cagcgagcca aggtgctcgc ggcgctgctg gaggcgacgc gccggcgcga ggatcgccgc
     9361 agcgtcctgg agcgcgtctg cgccgcctgc gtcgaggtca tgggcgagcc tgccgtgctc
     9421 gcggccgtca gcacgcggca gggggagagc atgctgctcg agtcggcggc aggcagggac
     9481 ggagccggcc gcccgctcga cgccttcgcc tactcgcccg cttcgttcga ggagtcggtg
     9541 ctccaggagg agcgggtcac ctcgctggac gacgccgcat cgcgcccgga cctgagcctg
     9601 ctgcgcgagg ccgacacgtc gttctcgtcg gctctctgcg cgcccttccg tctgggggga
     9661 aagcccggcg gcgcgctctg cgtctacggc cggcgcgccc accagtggac caccgagcag
     9721 tacgacctca tggggtggct cgccgaccag tgcggccggg tcatcgagag catgcggctg
     9781 caggagagcc tgcgcgagag cgagcgcgcg cagcgtgagc gcgaggagat cctccagagc
     9841 ttctaccaga gcgcctcgtt cctcatgggt gtcgcggagc tcgacggcga cgccgccgtc
     9901 gccgtctccg gtaatcgcgc catggaggag ttcttcgcgg ccgcccccgg agagccggga
     9961 tccggggtcg tgacgctgga cggcgctccg ggtctcgagg cacggctggc cgaacgcttc
    10021 cgcgagagcc aggcccgcgg gcgcccggtg cgcttcgagc acgtcgtcgc gagaggtggg
    10081 gtgacgcgca cgctcagcgt caccgtcgct cccatcggca cggccaccca cgggcggccg
    10141 ctgttcagct tcgtcgccga ggacgtgagc gagcgccgcc gtgccgagac ggcgctgcgc
    10201 gagcgcgagc tggcctcggc ggcgcaggag gagcgcagcc gcctcgcccg cgacctgcac
    10261 gactcggtga cccaggcgct gttcgcggcc agcctcaagg ccgaggcgct gaccggcgcc
    10321 ggcgatctcg tatcgccaca ggtcgtcacc accgtcgagg aggtgcgccg cctcagccgt
    10381 ggcgcccttg cgcagatgcg gaccatgctg ctcgagctgc gtggcgaccc gctcgaggag
    10441 gtacccatcg accagttgct gcgcaatgtg gtcgaggcca cggagagccg taccagcacc
    10501 gcggtcgcgc tcacgatgag cggggccgcg ccgctgccgg cgccgctgca cgtcgccctc
    10561 taccgcatct cgcaggaggc gctgaacaac gtcgcccgcc atgcccatgc tgcccacgcc
    10621 tgggtgcatc tcgagcttgc gcccgggcgc gcgcgcctct ccgtgagcga tgacggcgag
    10681 gggttcaccg acggcccggt cgcacctacg cacctcggcc tgcgctcgat gcgcgagcgc
    10741 gcggaagagg ccggcgcggc cttctacctg gccggcagac ccggcggcgg gacgctggtg
    10801 acggtggagt ggcgcggtgg cgaggcggag gccgggtgcg aggacggaga cggagcggac
    10861 ggggaaggct gctgaggcgc ggcgccggcg ggtgccgtcg gcgccgcgcc ccctgctgtg
    10921 tcgtcaggcg cgactgcggt tcaccagcag catgatgagc cagatgatga acagcgctat
    10981 ggcgacgtag aagagccact tgacgacgaa ccccagacca aagatgacga tcacgaggat
    11041 gagcaaaagg atccagagca acatgtcgta tctcctccgt tctctcccag gcctgttccg
    11101 ttcttacccg ggcttcaact ccccagccgg ccctggtgtt ttcgaccgtc ccgcgcagcg
    11161 accgtccgca aggatggcct tcgtgccggg cacttgaccc ccggccgtct gaccgaggca
    11221 aagaccgtcc ctccgaccac ccacgattcc gtccttccgg cccatacggc gcccgttgtg
    11281 cacgggaagg ctctcagtgc ccgccgtttg ggccgcccgt cggcgatacg aaaggaacgg
    11341 aaacttgagc acgccagtaa cgagctctct caatccctgg ggcccgatat cccagtccca
    11401 tcgcgtgcgc atctaccagc acggacgcac ctgcgaacac cctgactgca ccaccgtcct
    11461 ctccgtctac aacccgtcca cgtactgcaa cgcccacgcc gcgctggcgg cgcgcatgca
    11521 gcgccgggga ctcgccagcc cgacgcggga cgtcgcctgc gagcactgcg gcgaggtctt
    11581 ccagacggcg aatcgggcgc gtcgttactg cagcgaccgc tgtcgcatgg cggccttcgc
    11641 gcgccgcaag cgggcggcgg agcgcgcgaa ggccaggcac cgtgagcagg aggtgctcac
    11701 ggagctggtc gtcggggccc gggtgggctg acgacggtcc gagggcgcgg tgg
//
LOCUS       DASUMX010000127         9553 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_325180, whole genome shotgun sequence.
ACCESSION   DASUMX010000127 DASUMX010000000
VERSION     DASUMX010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 9553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9553
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325180"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VFZ86_06305"
     CDS             complement(<1..186)
                     /locus_tag="VFZ86_06305"
                     /inference="COORDINATES: protein motif:HMM:NF013112.4"
                     /note="catalyzes the formation of
                     2-methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at
                     position 37 in tRNAs that read codons beginning with
                     uridine from N6-(dimethylallyl)adenosine (i(6)A); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEX5641939.1"
                     /translation="MTGRYYLRSFGCQMNEHDAERIRALLEARGLLETTAPDDADVLV
                     YNTCTVRKSADERLAGHL"
     gene            complement(227..1759)
                     /gene="rny"
                     /locus_tag="VFZ86_06310"
     CDS             complement(227..1759)
                     /gene="rny"
                     /locus_tag="VFZ86_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006191971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEX5641940.1"
                     /translation="MMTAVYIVIGLAVGVAAGYFVRRNLSSSKLAGAEREAEKLLKDA
                     RREAETIVKEARLEAKEEVHRVRSEVEGELRERREELTKAEQRMMQREEAIDARGAEQ
                     DRREQSIRDRDANSQRISDEINNAHAEAMIQLERISGLSAAQAKEMLLKQTEDEARHE
                     MAKLVRQIEEEARREADRRARNILSLSIQRTAANHVAETTVSVVNLPSDDMKGRIIGR
                     EGRNIRTLENITGIDFIIDDTPEAVVLSGFDGVRRETARLTLTKLITDGRIHPARIEE
                     TYTAAKAEVEAAMEEAGEQACFDTNVHGVAPELVKVLGRLKYRTSYGQNVLNHSIEVA
                     HLAGVMAAELGANVKIAKRAGLLHDIGKAVDHEVDGSHADISQQLCKKYRESQSVCHA
                     IQAHHQDVEPQTVEAVLVQAADAISAARPGARRESLENYIKRLESLEHIAEAHKGVEK
                     CFAMQAGREVRVMVKPAEIDDDATALLAREIAREIEDQLDYPGQIRVTVIRESRAVDI
                     AK"
     gene            complement(2054..2692)
                     /locus_tag="VFZ86_06315"
     CDS             complement(2054..2692)
                     /locus_tag="VFZ86_06315"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HEX5641941.1"
                     /translation="MPVVTALRATRRGGVALHVDDKYVCVVSESLVARWRLFKGRELD
                     APDVAAIRAASSAERVMGDAYRLLGHRARSREELRRRLLAKDHDEHAVEDALEKLSAD
                     GFLDDAAFARSYAADKRRIGGWGEARIRRGLRELGVAAADADDALGAQGGGEGEELER
                     ALAVLRRRGAPEQPLDAARRRALQALQRRGFSSSVAYAAVRLWAEGEPPRPN"
     gene            complement(2695..3768)
                     /gene="recA"
                     /locus_tag="VFZ86_06320"
     CDS             complement(2695..3768)
                     /gene="recA"
                     /locus_tag="VFZ86_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018838103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEX5641942.1"
                     /translation="MDKDKALEAAVTQIERQFGKGSVMRMGDHPVSDEMQIVPSGSLA
                     LDIALGVGGIPRGRIVEIYGPEASGKTTLVYHLIAQAQKLGGQAAFIDAEHAMDPIYA
                     KTIGVDVDNLLVSQPDYGEQALEIAEMLVRSGALDIVAIDSVAALTPKAEIEGEMGDS
                     FVGLQARLMSQALRKLAGTLNRSGTICVFTNQLREKIGVMFGNPEVTTGGKALKFYAT
                     VRLDIRRIETLKDGTEAVGNRCRVKVVKNKVAPPFKQAEFDIMYGQGISREGSLLDLG
                     VLYDVVAKSGAFFSYNDERLGQGRANAKVFLRENPDIADAIEAQVRTKAELPGSAPAE
                     DAAPAAPAVPDGVDPVTGEVLQS"
     gene            complement(3788..4348)
                     /gene="thpR"
                     /locus_tag="VFZ86_06325"
     CDS             complement(3788..4348)
                     /gene="thpR"
                     /locus_tag="VFZ86_06325"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEX5641943.1"
                     /translation="MNGAAPARPARLRLFVACDLPPEVERAVAAWQREELGRHEDLRL
                     LPALHLTLAFLGSLEAARVPDLERVLRAITWTRAECRFREPLFLPARGKRHVVALELD
                     DPAGALRRLQADVAAGLAAEGLYEPEERPWLPHVTVARFRRPGHPFSLQNVNIGGFGV
                     VRMVLYSSHLERAGAVHTPVADFPAS"
     gene            complement(4345..5595)
                     /locus_tag="VFZ86_06330"
     CDS             complement(4345..5595)
                     /locus_tag="VFZ86_06330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HEX5641944.1"
                     /translation="MPPGAPRAAVVLSGNELLDGRTRDTNGAFVCDDLSRRGVKVTEL
                     LTVADDRDRLAAALRHALGGAPELLVIGGGLGTTHDDLTAECLAEVLGVPLEEDAGAL
                     AYVEERSRAVAARRHLDFAETFTLARRQARLPAGSTAVPPAGVAPGIAARAGRTRIFA
                     YPGVPYEFQQMWLETAAALEHDGFFPDVVVRVVRVFGVGELQVGPVLDALPHDLVELG
                     VNVGGGEVTVRIRHRRGPGGQAQADALVAALEAGVPVYSTDGRTVDDLIAGALRGRGL
                     TLAVAESCTGGLLGSRITARPGSSDYFLGGVISYGNQAKVDLLDVPAAMLARHGAVSE
                     EVAGAMAAGARARLGADYALSVTGVAGPDGGTPDKPVGLVYLGCAGPEGTQVHRGSFP
                     GDRESVRTFSATTALHLLRRALAP"
     gene            complement(5595..6185)
                     /gene="pgsA"
                     /locus_tag="VFZ86_06335"
     CDS             complement(5595..6185)
                     /gene="pgsA"
                     /locus_tag="VFZ86_06335"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012447923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEX5641945.1"
                     /translation="MSSQLNLANSITIARILLVPVFLVILLSGLPQPYGDLLAAAVFI
                     LAAATDKLDGYVARRSKQVTTLGQFLDPLADKLLIAAALIALVAQDRVAAWVAMVIIG
                     REIAVSVLRIIGVSQGVSIPADKYGKLKTVLQIVYVVYVLFPTADIAELIHVSTDVAV
                     IIQWILAVVVVGVTLASGVNYFRNARGLIRMPGTSG"
     gene            complement(6182..7528)
                     /locus_tag="VFZ86_06340"
     CDS             complement(6182..7528)
                     /locus_tag="VFZ86_06340"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HEX5641946.1"
                     /translation="MGHPGPVRQLPLRPVTGSSFFIQTLGCPKNEADSDALEARLRAA
                     GHRPAAAGRAAVVVVNTCGFIDAAKEESIEAILDASAAAHARGARVAAVGCLVERYRD
                     ELAAEIPEVDLWCGLDTAPLLAALAAGEATATAGGAGAAVPVPRRPRPVSAYVKISDG
                     CDRRCSFCAIPLIKGDYETVAAAEVLRTARAALAAGARELVLVGQDTSRWAQPGWGGV
                     SRLLGELAALEPAPDWLRLLYLQPEGIDDALLEALAAHAVPYVDVPLQHASGAVLRRM
                     RRSGDGDAHLALLQRIRAALPGAAVRSTFIAGFPGETDAEFEELVGFVRDAGLAVAGV
                     FVYDEQEGTAAAGMPGAVPHQLALERAARLGEVIDREAERFWSDLAGATVDVLVERGA
                     SRPGDAAIGRIALQAPDVDGRTTVRGARVRRGDLVSAVVGDTLGYDVEAVAATGGP"
     gene            complement(7492..7983)
                     /locus_tag="VFZ86_06345"
     CDS             complement(7492..7983)
                     /locus_tag="VFZ86_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEX5641947.1"
                     /translation="MAKEFSFDVVSRADLNEVRNAVNMAQKEIATRYDFKKSVSSVTL
                     EGEALVLVSDNDGKLKQVVDVLEEKLVRRKVPLKAIQYGKVEEALGGTVRQRAPIAQG
                     IPDEKAKAMNKLLRQRYKKVKVQIQGDTVRVFSASKDELQAAMGALRDEDWGIPVQFV
                     NYR"
     gene            complement(7987..8757)
                     /locus_tag="VFZ86_06350"
     CDS             complement(7987..8757)
                     /locus_tag="VFZ86_06350"
                     /inference="COORDINATES: protein motif:HMM:NF024805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HEX5641948.1"
                     /translation="MFEIGTTLREARVRRALTLQQVEEDIKIRVKYVQAMENEDWDVM
                     PGVTYVKGFLRTYSTYLGLDPEVIIGEFRSRAIAPSAEHHEPFGGSSVIGKPHGHRGR
                     NTVVVVAVVCLLALAVIYALGLMNGDGGEEPTTTPGALGIASPSPSPSASPTPSPSAT
                     GVPAWQKDLVKIEAVDGDCWMEVRRNNSTGVVLFSGTLKKGDMQKFKGKDIWMSLGAP
                     ANVELVAAGKKAKLKSDVGPWTVEVIRGRVVQGPETQG"
     gene            complement(8873..>9553)
                     /locus_tag="VFZ86_06355"
     CDS             complement(8873..>9553)
                     /locus_tag="VFZ86_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEX5641949.1"
                     /translation="RSCGIHLVVATQRPSVDVVTGMIKTNIPSRIAFAVASQTDSRII
                     LDQGGAESLLGMGDMLFSPMGSSKLLRVQGALVTSAEMKLVTEHWKRQAKPDYHEELL
                     ENPAGPDAEQSAQSAGGDELLGEAIKTVVNTGAASVALLQRRLRVGYARAGRLIDIME
                     EMGIISGYDGSKARAVLIDEAGLPMALARLSGDVVDDPVADAGEPETAAPAPEGPPPA
                     AEPAPADL"
BASE COUNT         1234 a         3361 c         3503 g         1455 t
ORIGIN      
        1 caggtgcccc gccagccgtt cgtccgcgct cttgcgcacc gtgcaggtgt tgtacacgag
       61 cacgtcggca tcgtcgggag cggtcgtctc caggaggccg cgcgcctcga gcagcgcgcg
      121 gatgcgctcg gcgtcgtgct cgttcatctg gcagccgaac gagcgcaggt agtaacggcc
      181 ggtcatggcg gtctcgtcgc gctggcgggg cggcgtcgcc gccccgttat ttggcgatgt
      241 cgacggcgcg gctctcgcgg atgacggtca cgcggatctg gccgggatag tccagctggt
      301 cctcgatctc gcgcgcgatc tcgcgcgcca ggagcgccgt ggcgtcgtcg tcgatctcgg
      361 ccggtttgac catgacgcgc acctcgcgcc ctgcctgcat cgcgaagcac ttctcgaccc
      421 ccttgtgggc ctcggcgatg tgctccagcg actccagccg cttgatgtag ttctcgagcg
      481 actcgcggcg ggcgcccgga cgggcggcgc tgatggcgtc ggccgcctgc acgagcacgg
      541 cctccacggt ctgcggctcc acgtcctggt ggtgcgcctg gatcgcgtgg cagacgctct
      601 gcgactcgcg gtacttcttg cagagctgct gcgagatgtc ggcgtgggag ccgtcgacct
      661 cgtggtccac ggccttgccg atgtcgtgca gcaggccggc gcgcttggcg atcttgacgt
      721 tggcgccgag ctcggcggcc atgacgccgg cgaggtgtgc gacctcgatg gagtggttca
      781 gcacgttctg gccgtagctg gtgcggtact tgaggcgccc gaggaccttg accagctcgg
      841 gcgccacccc gtgcacgttg gtgtcgaagc aggcctgctc gccggcctcc tccatggcgg
      901 cctcgacctc ggccttggcc gcggtgtacg tctcctcgat gcgcgccggg tggatgcgtc
      961 cgtcggtgat gagcttggtg agcgtgagcc tggcggtctc ccggcgcacg ccgtcgaagc
     1021 cggagagcac gaccgcctcc ggcgtgtcgt cgatgatgaa gtcgatgccg gtgatgttct
     1081 cgagggtgcg gatgttgcgg ccctcgcggc cgatgatgcg gcccttcatg tcgtccgagg
     1141 gcaggttgac gacggagacc gtggtctccg cgacgtggtt ggcggcggtg cgctggatgc
     1201 tgagcgagag gatgttgcgg gcgcgcctgt ccgcctccct gcgcgcctcc tcctcgatct
     1261 ggcggacgag cttggccatc tcgtggcgcg cctcgtcctc cgtctgcttg agcagcatct
     1321 ccttggcctg cgcggcgctg aggccggaga tgcgctcgag ctggatcatc gcctcggcgt
     1381 gcgcgttgtt gatctcgtcc gagatgcgct ggctgttggc gtcgcggtcg cggatggact
     1441 gctcgcggcg gtcctgctcc gcgccgcgcg cgtcgatggc ctcctcgcgc tgcatcatcc
     1501 gctgctcggc cttggtgagc tcctcgcggc gctcgcgcag ctctccctcg acctcgctgc
     1561 gaacgcggtg gacctcctcc ttggcctcga ggcgggcctc cttgacgatg gtctccgcct
     1621 cgcggcgcgc atccttgagc agtttctctg cctcgcgctc ggcgccggca agcttgctcg
     1681 aggagaggtt gcggcggacg aagtaccccg ccgcgacgcc caccgcgagc ccgatcacga
     1741 tatagaccgc tgtcatcatt ctctctgtcc cttcacaggt cgcgcggcac gaaaaaaggc
     1801 cgggcaacgc gcccggcctg tgccacgctc gcacctgcac cacgacaaat ccagctgtga
     1861 ctgcgcccag gggtcaggac ggctgcgggt tcgcgaaacc tcccggttct gtcatcggtg
     1921 acccctggtt gatcggttca gcttgatcgg cgccttggcg ccgtctatat cgccatgcaa
     1981 gagcgagagt ggaatacaca aagcacgcgc acacgtcgcg tgtttcgatg ttacgtgacg
     2041 ccgcctcgcg gagtcaattc ggccggggcg gctcgccctc ggcccagaga cgcaccgcgg
     2101 cgtaggcgac ggaggacgag aacccgcggc gctggagcgc ctggagggcg cgccgccggg
     2161 ccgcgtcgag cggctgctcc ggcgcgccgc gccggcgcag gacggcgagg gcgcgctcga
     2221 gctcctcgcc ctctccccca ccctgcgccc ccagcgcgtc gtcggcgtcg gcggcggcga
     2281 cgccgagctc gcgcaggccg cggcggatgc gtgcctcgcc ccagccgccg atccggcgct
     2341 tgtccgccgc gtagctgcgc gcgaacgcgg cgtcgtcgag gaagccgtcc gcggacagct
     2401 tctccagggc gtcctcgacg gcgtgctcgt cgtggtcctt ggcaaggagc cgccggcgga
     2461 gctcctcgcg gctccgggcg cggtggccga gcagacgata ggcgtcgccc atgacccgct
     2521 ccgccgacga ggcggcacgg atggcggcca cgtccggcgc gtcgagctcg cggcccttga
     2581 agaggcgcca gcgggccacc agcgactcgc tgacgacgca cacgtacttg tcgtccacgt
     2641 gcagcgccac ccctccgcgg cgggtggcgc gcagggcggt gacgacgggc accgctagct
     2701 ctgcaggacc tcgccggtga cggggtcgac gccgtcgggg acggccggcg ccgccggcgc
     2761 ggcgtcctcg gccggcgcgc tgcccggcaa ctccgccttg gtgcgcacct gcgcctcgat
     2821 ggcgtcggcg atgtccgggt tctccctgag gaagaccttg gcgttggcgc ggccctgacc
     2881 caggcgctcg tcgttgtagc tgaagaaggc gccgctcttg gcgacgacgt cgtagagcac
     2941 gccgaggtcg agcagactgc cctcgcggga gatgccctgg ccgtacatga tgtcgaactc
     3001 ggcctgcttg aagggcgggg cgaccttgtt cttgacgacc ttgacccggc agcggttgcc
     3061 gaccgcctcg gtgccgtcct tgagcgtctc gatgcggcgg atgtcgagcc tgacggtggc
     3121 gtagaacttg agcgccttgc cgcccgtggt gacctcgggg ttgccgaaca tgacgccgat
     3181 cttctcgcgc agctggttgg tgaacacgca gatggtgccg gaacggttga gcgtgcccgc
     3241 gagcttgcgc agggcctggc tcatgagccg cgcctgcagg cccacgaagc tgtcgcccat
     3301 ctccccctcg atctcggcct tgggggtgag cgcggcgacg gagtcgatgg cgacgatgtc
     3361 gagggcgccg ctgcgcacca gcatctcggc gatctccagg gcctgctcgc cgtagtccgg
     3421 ctgcgagacc agcaggttgt ccacgtcgac gccgatggtc ttggcgtaga tggggtccat
     3481 ggcgtgctcg gcgtcgatga acgccgcctg cccgccgagc ttctgcgcct gggcgatgag
     3541 gtgatagacg agggtggtct tgcccgaggc ctccgggccg tagatctcga cgatgcggcc
     3601 gcgcgggatg ccgcccacgc cgagcgcgat gtcgagtgcc agcgacccgc tcgggacgat
     3661 ctgcatctcg tcgctcacgg ggtggtcgcc catgcgcatg acactgccct tgccgaactg
     3721 gcgctcgatc tgggtgacgg cagcctcgag ggctttgtct ttgtccaagg tagcgctcct
     3781 ctcttgctca ggacgcggga aagtctgcta cgggggtgtg cactgctccg gcccgttcca
     3841 ggtggctact atacaagacc atccggacga caccaaaccc gccgatgttc acattttgca
     3901 gggaaaatgg gtgacctgga cggcggaatc tggcgacggt gacgtgcggc agccaggggc
     3961 gctcctcggg ctcgtacagg ccttcggccg cgagcccggc ggccacgtcc gcctgcagcc
     4021 ggcgcagggc gccggcgggg tcgtcgagct cgagcgcgac gacgtgccgc ttgccgcgcg
     4081 ccggcaggaa cagcggctcg cggaagcggc actccgcgcg cgtccaggtg atggcgcgca
     4141 ggacacgttc gaggtccggc acccgggcgg cctcgaggct gccgaggaac gcgagcgtga
     4201 ggtgcagggc cggcaggagc cggaggtcct cgtgccggcc cagctcctcg cgctgccacg
     4261 cggcgacggc gcgttcgacc tccgggggca ggtcgcaggc cacgaacagg cgcaggcgcg
     4321 ccggccgggc cggcgcggcg ccgttcacgg cgcgagcgcg cggcgcagca ggtgcagcgc
     4381 ggtggtggcg ctgaaggtgc gcacgctctc gcggtcgccg gggaacgagc ccctgtggac
     4441 ctgcgtgccc tccggcccgg cgcacccgag gtagaccaga ccgaccggct tgtccggcgt
     4501 gccgccgtcc gggccggcca cgccggtgac ggagagggcg tagtcggcgc cgagccgggc
     4561 gcgcgcgccg gcggccatcg ccccggccac ctcctccgac accgcgccgt gccgcgccag
     4621 catggcggcc gggacgtcca ggaggtcgac cttcgcttgg ttgccgtagc tgatcacgcc
     4681 gccgaggaag tagtcggagc tgcccggcct ggcggtgatg cgggagccga gcaggccgcc
     4741 ggtgcacgac tcggccaccg cgagcgtcag gccgcggccg cgcagggcgc cggcgatgag
     4801 gtcgtccacg gtgcggccgt cggtcgagta caccggcacc ccggcctcga gggccgccac
     4861 gagcgcgtcc gcctgcgcct gcccaccggg tccgcgccgg tggcggatgc gcacggtcac
     4921 ctctcccccg ccgacgttga cgccgagctc gaccagatcg tgcggcagcg cgtccaggac
     4981 gggcccgacc tgcagctcgc cgaccccgaa cacgcggacc acgcgcacga cgacgtccgg
     5041 gaagaagccg tcgtgctcga gcgccgccgc cgtctcgagc cacatctgct gaaattcgta
     5101 gggcacgccg ggataggcga agatgcgcgt ccgccccgcg cgcgccgcga tgccgggcgc
     5161 gacgccggcc ggcgggacgg cggtggagcc ggccggcagc ctcgcctggc ggcgcgccag
     5221 cgtgaaggtc tccgcgaagt cgaggtgccg gcgcgcggcg acggcgcggg agcgctcctc
     5281 gacgtaggcg agcgcgcccg cgtcctcctc cagcgggacc ccgaggacct ccgccaggca
     5341 ctcggcggtg aggtcgtcgt gcgtggtgcc cagcccgccg ccgatcacca ggagctcggg
     5401 cgcccctccg agcgcgtgcc ggagggcggc ggcgagccgg tcgcggtcgt cggccaccgt
     5461 cagcagttcc gtcaccttga cgccgcgacg gctcaggtcg tcgcagacga aggcgccgtt
     5521 ggtgtcgcgg gtgcggccgt cgagcagctc gttgccgctg aggaccacgg ccgcgcgcgg
     5581 cgctcccgga ggcatcagcc gctggtcccg ggcatgcgga tgaggccccg ggcgttcctg
     5641 aagtagttca ctccgctcgc cagggtcacg ccgacgacca cgacggcgag gatccactgg
     5701 atgatcacgg ccacgtcggt ggagacgtgg atgagctccg cgatgtcggc cgtcgggaag
     5761 agcacgtaca ccacgtacac gatctgcagc acggtcttga gcttgccgta cttgtcggcg
     5821 gggatggaga ccccctgtga gacgccgatg atgcgcagca cgctcacggc gatctcccgc
     5881 ccgatgatga ccatggcgac ccaggccgcc acgcggtcct gcgccaccag ggcgatgagc
     5941 gcggcggcga tgagcagctt gtcggcgagg gggtcgagga actgcccgag cgtggtgacc
     6001 tgcttgctcc gccgcgccac gtagccgtcg agcttgtcgg tggccgcggc gaggatgaag
     6061 acggccgccg cgagcaggtc gccgtagggc tgcggcaggc ccgagaggag gatcaccagg
     6121 aacaccggga cgagcaggat ccgtgcgatg gtgatgctgt tggcgaggtt gagctggctg
     6181 ctcacggtcc ccctgtcgcg gcgacggcct ccacatcata cccgagggtg tcgccgacga
     6241 cggcagagac gaggtcgccg cggcggacgc gggcgccgcg gaccgtcgtg cggccgtcga
     6301 cgtccggcgc ctgcagggcg atgcggccga tcgcggcgtc gccggggcgc gacgcgccgc
     6361 gctccacgag gacgtcgacc gtggcgccgg cgaggtccga ccagaagcgc tccgcctcgc
     6421 ggtcgatgac ctcgccgagg cgggcggcac gctcgagggc gagctggtga ggcacggcgc
     6481 cgggcatgcc ggccgccgcc gtgccctcct gctcgtcgta gacgaagacg ccggccacgg
     6541 cgagcccggc gtcgcggacg aagcccacga gctcctcgaa ctccgcgtcg gtctcgcccg
     6601 ggaacccggc gatgaacgtg gagcgcacgg cggcgccggg cagcgcggcg cggatgcgct
     6661 gcaggagcgc cagatgggca tccccgtctc ccgagcgcct catgcggcgc aggacggcgc
     6721 cggaggcgtg ctggaggggg acgtccacgt agggcacggc gtgcgccgca agggcctcca
     6781 ggagggcgtc gtcgatgccc tccggctgca ggtagagcag gcgcagccag tcgggggcgg
     6841 gctcgagggc cgccagttcg cccagcaggc ggctcactcc gccccacccc ggctgcgccc
     6901 agcgcgaggt gtcctggccg acgagcacca gctcgcgggc gccggcggcc agggccgcgc
     6961 gcgccgtgcg cagcacctct gcggcggcga ccgtctcgta gtcgcccttg atcagcggga
     7021 tggcgcagaa cgagcagcgc cggtcgcagc cgtccgagat cttgacgtag gcgctgaccg
     7081 ggcgcggccg gcgcgggacg ggcaccgccg cgccggcccc cccggccgtc gcggtcgcct
     7141 cgccggccgc gagcgccgcg aggagcggcg ccgtgtccag gccgcaccac aggtccacct
     7201 ccgggatctc ggcggcgagc tcgtcgcggt agcgctccac gaggcagccg acggcagcca
     7261 cccgcgcgcc gcgggcgtgg gcggccgcgg aggcgtcgag gatggcctcg atcgactcct
     7321 ccttggcggc gtcgatgaag ccgcaggtgt tgacgacgac gacggcggcg cggccggccg
     7381 ccgcggggcg gtgccccgcg gcgcggagcc gcgcctcgag cgcgtcggag tcggcctcgt
     7441 tcttcgggca gccgagggtc tggatgaaga acgagctgcc cgtgacgggc ctcagcggta
     7501 gttgacgaac tggaccggga tgccccaatc ctcgtcacgc agggcgccca tggccgcctg
     7561 gagctcgtcc ttgctggccg agaacacgcg cacggtgtcg ccctggatct ggaccttgac
     7621 cttcttgtag cgctggcgca ggagcttgtt catcgccttg gccttctcgt ccgggatgcc
     7681 ctgggcgatg ggcgcgcgct gccggacggt gccgccgagg gcctcctcca ccttgccgta
     7741 ctggatggcc ttgagcggca ccttgcggcg cacgagcttc tcctcgagca cgtcgacgac
     7801 ctgcttgagc ttgccgtcgt tgtcggagac gagcacgagc gcctcaccct ccagcgtcac
     7861 cgagctcaca ctcttcttga agtcgtaccg cgtcgcgatc tccttctgcg ccatgttcac
     7921 ggcgttgcgc acctcgttga gatccgccct ggagaccacg tcgaacgaga attccttggc
     7981 catgtgctac ccctgcgtct ctggaccctg gacgacgcgc ccccggatga cctcgaccgt
     8041 ccacgggccg acgtcgctct tcagcttcgc cttcttgccg gcggccacga gctccacgtt
     8101 ggcgggagcg ccgaggctca tccagatgtc cttgcctttg aacttctgca tgtcgccctt
     8161 cttgagcgta cccgagaaca gcacgacgcc ggtgctgttg ttgcgccgca cctccatcca
     8221 gcagtccccg tccacggcct cgatcttgac gaggtccttc tgccaggcgg ggacgccggt
     8281 cgcgctcggc gacggcgtgg gcgaggcgct cggcgacggc gacgggctgg cgatgccgag
     8341 cgcgccgggc gtcgtggtgg gctcttcgcc gccgtcgccg ttcatgaggc cgagcgcgta
     8401 gatgaccgcc agggcgagga ggcagaccac ggcgacgacc acgacggtgt tgcggccgcg
     8461 gtggccgtgc ggcttgccga tgaccgacga gccgccgaac ggctcgtggt gctcggcgga
     8521 cggggcgatg gcgcgggagc ggaactcgcc gatgatgacc tccgggtcga ggccgagata
     8581 ggtggagtac gtgcgcagga agcccttgac gtaggtgacc cccggcatga cgtcccagtc
     8641 ctcgttctcc atggcctgga cgtacttgac ccggatcttg atgtcttcct cgacctgctg
     8701 cagcgtgagg gcgcggcgga cgcgggcctc gcgcagcgtg gtgccgatct caaacacggt
     8761 ggatgccgtt ctcctcgctc gccgctgggg gtccggtcgc ggcgctcacc cgaacgtaca
     8821 gtctagtaac cgggtgctgg gatgacaaac gagctccgag gctgcgcgag gctcagaggt
     8881 cggcgggcgc cggctcggcc gccggaggcg gcccctccgg cgccggggcc gcggtctcgg
     8941 gctcgccggc gtccgccacg gggtcgtcca cgacgtcgcc gctgaggcgc gcgagcgcca
     9001 tcggcagacc cgcctcgtcg atgagcaccg cgcgcgcctt gctgccgtcg tacccggaga
     9061 tgatgcccat ctcctccatg atgtcgatga gtcgtccggc gcgcgcgtag cccacgcgca
     9121 ggcgccgctg cagcagcgcc acgctcgccg cacccgtgtt caccacggtc ttgatggcct
     9181 cgccgaggag ctcgtcgccg ccggccgact gcgccgactg ctcggcgtcg gggccggccg
     9241 ggttctcgag cagctcctcg tggtagtcgg gcttggcctg gcgcttccag tgctcggtga
     9301 cgagcttcat ctcggcgctc gtcaccaggg cgccctgcac gcgcagcagc ttgctgctcc
     9361 ccatggggct gaacagcatg tcgcccatgc ccagcaggct ctccgccccg ccctggtcga
     9421 ggatgatgcg ggagtcggtc tgcgaggcca cggcgaaggc gatgcgcgag gggatgttgg
     9481 tcttgatcat gccggtgacc acgtcgaccg acgggcgctg cgtggccacc acgaggtgga
     9541 tgccgcagct gcg
//
LOCUS       DASUMX010000128         2269 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_469740, whole genome shotgun sequence.
ACCESSION   DASUMX010000128 DASUMX010000000
VERSION     DASUMX010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469740"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="VFZ86_06360"
     CDS             complement(<1..330)
                     /locus_tag="VFZ86_06360"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HEX5641950.1"
                     /translation="MTDLLARTIAAIGPLDADAMRAAEARQLQLTKPPKALGRLETLS
                     IQLAGIQGREQPVIEQKAIAVMAADHGVVAEGVSAFPAEVTPAMVLNMVAGGAGINVI
                     GRHVGARV"
     gene            complement(327..1091)
                     /locus_tag="VFZ86_06365"
     CDS             complement(327..1091)
                     /locus_tag="VFZ86_06365"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019542053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008820 - cobinamide phosphate
                     guanylyltransferase activity [Evidence IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HEX5641951.1"
                     /translation="MDRAQDRPVRGRLILVTGGARSGKSTYAERLAAQLAEPHGGRVT
                     YIATSQTYDEEMEQRVTAHQASRPETWTTVECPVEVPAAVRAAAAGVGEGGAGAAGPT
                     GSPPVILLDCVTLWVTNLLFSGGAFGGSEPPEGGYNYDKDLLPPAEERAAAARVTAAV
                     TGLIAAVAETGVTLVAVTNEVGLGVVPEYPLARLYRDQLGWANQRLARDADGLYLLVS
                     GHPLDVKALAAGAPAAAGPSSTPSPDDLSSPPKESQ"
     regulatory      complement(1135..1257)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(1373..>2269)
                     /locus_tag="VFZ86_06370"
     CDS             complement(1373..>2269)
                     /locus_tag="VFZ86_06370"
                     /inference="COORDINATES: protein motif:HMM:NF012800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEX5641952.1"
                     /translation="VPAAVGAQLAHDGRAEHGQLVHASLAAVVAAARGARLARHGARA
                     LTSRCGAVDILEAVGVDVDCDVTAVERSIGLAGIGLFNGMSPAVHPGGLGRILSQIRF
                     GSTLNIAASLANPVRPTHGVRGVYAEGLLGPVGSVMMEIGYEHALVVHGYDDRREAGM
                     DELSVLGATVVSELRADGRRDSYTVEPEDFGLRRAAHGEIAPLGDLRAEAVRFLQVLG
                     GRGHEACGDAACLNAAAVLYVSGQAADLGDGLAQAREAVASGAALAKLEEWVAAQAGG
                     LEQRSAGEARLAALRAEAGLRS"
BASE COUNT          268 a          839 c          856 g          306 t
ORIGIN      
        1 gacgcgggcg ccgacgtgcc ggccgatgac gttgatgccg gcgccgccgg cgaccatgtt
       61 gaggaccatc gccggcgtga cctcggcggg aaaggcgctg acgccctcgg cgacgacgcc
      121 gtggtcggcc gccatgacgg cgatcgcctt ctgctcgatg accggctgct ccctgccctg
      181 gatgccggcc agctggatcg agagcgtctc gaggcggccg agcgccttgg gcggcttggt
      241 gagctgcagc tggcgtgcct ccgcagcgcg catggcgtcg gcatcgagcg ggccgatggc
      301 ggcgatggtt ctggcgagca ggtcggtcac tgggactcct ttgggggtga cgagagatca
      361 tccggggatg gggtggacga ggggccggcc gccgcaggcg ctccggccgc cagcgccttg
      421 acgtcgagag ggtggccgga gacgagcagg tagagaccgt cggcgtcgcg ggcgaggcgc
      481 tggttggccc atcccagctg gtcgcggtac aggcgagcga gggggtactc ggggacgacc
      541 ccgagcccca cctcgttggt gacggcgacc agggtcacgc cggtctcggc gacggcggcg
      601 atcagaccgg tgacggcggc cgtgacgcgc gccgcggcgg cgcgctcctc ggccggcggc
      661 agcaggtcct tgtcgtagtt gtagccgccc tccggcggct cgctgccgcc gaaggcgccg
      721 ccgctgaaga ggaggttggt cacccagagg gtgacgcagt cgagcaggat gacgggcggg
      781 gagccggtgg ggcctgcggc tccggcgccc ccttcaccca cgccggcggc ggccgcgcgc
      841 actgccgccg gcacctcgac cgggcactcg accgtggtcc acgtctccgg ccgcgaggcc
      901 tggtgcgcgg tcacgcgctg ctccatctcc tcgtcgtagg tctgagacgt ggcgatgtag
      961 gtcacccggc cgccgtgcgg ctcggccagc tgcgccgcga gccgctccgc gtacgtgctc
     1021 ttgccgctcc gcgcgccgcc ggtgacgagg atgagacggc ctcgaacggg ccggtcctgt
     1081 gctctgtcca ccgcatgccc tccctcgagg cgttgtcgca gtgatctccc gctgaggttt
     1141 cctggctcgc gcttcgtcct acttgtcgcg ccttcccgcc cgagggcagt ggcgtgcctg
     1201 cgacgttcgt ccgcgcctac agttgcgggg cagcgccggc cttgcaccgg cttccctgca
     1261 gctgggagat gcccggcaag gctatcgccg ttgcgtcgcg ggcgtcaatc aggcgctcgc
     1321 gcctgccgag gcggcggggc ggacgtcgcg accgcccccg cgcgccccgc cgtcaggacc
     1381 ggaggcccgc ctccgcgcgc agcgccgcca ggcgggcctc gccggcgctg cgctgctcga
     1441 gtccgccggc ctgcgccgcg acccactcct cgagcttggc gagcgccgcc ccgctcgcga
     1501 ccgcctcgcg cgcctgcgcg agcccgtcgc cgaggtcggc ggcctgaccc gacacgtaga
     1561 ggacggcggc cgcgttgagg caggcggcat cgccgcacgc ctcgtggccg cggccgccca
     1621 gcacctgcag gaagcgcacg gcctcggcgc gcaggtcgcc cagcggcgcg atctcgccgt
     1681 gtgccgcgcg ccgcagcccg aagtcctcgg gctcgaccgt gtacgagtcc cggcggccgt
     1741 cggcgcgcag ctcgctcacg acggtcgcgc cgagcacgga cagctcgtcc atgcctgctt
     1801 cacgccggtc gtcgtagccg tgtacgacca gcgcgtgctc gtacccgatc tccatcatca
     1861 cgctcccgac gggtcccagc agcccttcgg cgtagacgcc gcgcacgccg tgggtcggcc
     1921 gcaccgggtt ggcgagcgac gcggcgatgt tcagcgtcga gccgaagcgg atctggctga
     1981 ggatgcggcc cagaccgccc gggtggacgg ccgggctcat gccgttgaag aggccgatgc
     2041 ccgcgaggcc gatactgcgc tccaccgcag tgacgtcgca gtccacgtcg accccgacgg
     2101 cctcgaggat gtccaccgcg ccgcaccgcg acgtgagggc acgcgcgccg tgccgcgcca
     2161 gccgggcgcc ccgggcggcg gcgacgaccg ccgcgaggga ggcatggacg agctgtccgt
     2221 gctcggcgcg accgtcgtga gcgagctgcg cgccgacggc cgcagggac
//
LOCUS       DASUMX010000129         2546 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1043468, whole genome shotgun sequence.
ACCESSION   DASUMX010000129 DASUMX010000000
VERSION     DASUMX010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1043468"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..1465)
                     /locus_tag="VFZ86_06375"
     CDS             complement(14..1465)
                     /locus_tag="VFZ86_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEX5641953.1"
                     /translation="MQAADAGSGGALERFFKFKQWDTSIKRDTLAGLATFMVMAYIIF
                     VNPNILGLGGAPEGLPFAAALTSTCLVAGVMTILMGLVTNRAYAIAPGMGLNAVVAFS
                     LVLGQGLTMAEAMGLIVLEGVAILILVLTGFREAIFKAIPIELKKAIVVGIGFFILFI
                     GLVDGGLINVNGGTPVGLVELGTVPMVPLAVTVFGLVVTIVMLALRWRAAILLGIVFA
                     TIFAIVLNYAWGKDAGFLVGQAIIPTDIVASPDFSLVGEFSFGAFGKLGITAAVLWIF
                     SLMLSDFFDTMGTLVGVGGEAGYLDKKGDLPQVNRPLLVDSLAAAAGGAVSASSATTY
                     IESGAGVAQGGRTGWVAIVVGVLFLVAMFFSPIFGVVPGQATAAALVVVGYLMMATLT
                     RAESEADHDTSEPKAGINFGNLAFGLPAVLIITIMPLTYSITNGIGAGFIAYVLIRIV
                     QGKAREVSWMLWVSSIAFIIYFIFPWLQAQGWV"
     gene            1643..>2546
                     /locus_tag="VFZ86_06380"
     CDS             1643..>2546
                     /locus_tag="VFZ86_06380"
                     /inference="COORDINATES: protein motif:HMM:NF015413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641954.1"
                     /translation="MDEIPGSLALSELDGMKVLDVAGAEVGELADVVASTATDRPVVT
                     AFFIDRDEGQLAASWAQVGEIDVDGERLVLGVPLEAVKAASLRPDELPLVDALLDNQV
                     LDMRRRAFVRVQDVVLAPDADHLVVAGVDASSAALARRFGLGFISRRLPKKSGDFVPW
                     NDVNLIALRLSRLNFVESFAELAELHPADIAEVISQVGTRERAAVLAALNASLAADTL
                     QEMEEGLRSAALQEMPLERAAQVLEHIEPDEAADVLSHLPDTLAQELLARLPDKREQD
                     LRRLASHPEHTAGSLMTTDFVMLPR"
BASE COUNT          429 a          862 c          891 g          364 t
ORIGIN      
        1 ggtcagcccg gtctcagacc cagccctgcg cctgtagcca cgggaagatg aagtagatga
       61 tgaaggcgat cgaggagacc cagagcatcc agctcacctc gcgcgccttg ccctgcacga
      121 tccggatgag cacgtaggcg atgaaccctg cgccgatgcc gttcgtgatg ctgtacgtga
      181 gcggcatgat cgtgatgatc agcacggcgg gcaggccgaa ggccaggttg ccgaagttga
      241 tgccggcctt gggctcgctc gtgtcgtggt ccgcctcgct ctcggccctg gtcagcgtcg
      301 ccatcatgag gtagcccacg acgacgaggg ccgccgcggt cgcctggccg ggcacgacgc
      361 cgaagatcgg tgagaagaac atggccacca ggaacagcac gcccacgacg atcgccaccc
      421 agccggtgcg gccgccctgc gccacgccgg cgccggactc gatgtaggtc gtggccgacg
      481 aagcgctgac ggcgccaccg gcggcggcgg cgagggagtc gacgagcagc gggcggttca
      541 cctgcgggag gtcgcccttc ttgtcgaggt aaccggcctc gccgcccacg ccgacaaggg
      601 tgcccatggt gtcgaagaag tcgctcagca tgaggctgaa gatccacagg acggccgccg
      661 tgatgccgag cttgccgaag gcgccgaagc tgaactcgcc gacgaggctg aagtccgggc
      721 ttgcgacgat gtcggtcggg atgatcgcct gccccacgag gaatccggcg tccttgcccc
      781 aggcgtagtt gaggacgatc gcgaagatcg tcgcgaagac gatgccgagc aggatcgcgg
      841 cgcgccagcg caacgccagc atgacgatgg tcacgacgag cccgaacaca gtcacggcga
      901 gtggcaccat cggcacggtc cccagctcga cgagcccgac cggcgtgccg ccgttgacgt
      961 tgatgagccc gccgtccacg agcccgatga agaggatgaa gaagccgatg cccacgacga
     1021 tcgccttctt gagctcgatc gggatcgcct tgaagatcgc ctcccggaag ccggtgagca
     1081 cgaggatcag gatggcgaca ccctcgagca cgatgaggcc catcgcctcg gccatcgtga
     1141 gcccctggcc gagcacgagg ctgaacgcca cgacggcgtt gagtcccatg ccgggggcga
     1201 tggcgtaggc gcggttggtc accagaccca tgaggatggt catgacgccg gccacgagac
     1261 acgtcgacgt gagcgccgcg gcgaacggga gaccctcggg ggcgccgccc agcccgagga
     1321 tgttcgggtt cacgaagatg atgtaggcca tgaccatgaa ggtcgcgagc ccggcgagcg
     1381 tgtcgcgctt gatcgacgtg tcccactgtt tgaacttgaa gaaccgctcg agcgctccgc
     1441 ccgagccggc gtctgccgct tgcatagaga cctcgtcctc cttcggggtc gatgggtggg
     1501 agaatgaccg cgagagcata cagcaccggg tgcctacgag cgctatcgtt gtggctggct
     1561 ccgacggcgc ggcgattgac ggtctcctgc ccgactggca ttctcgtcgg gcaccccccc
     1621 acgaaaggac gtgcaccgct ccatggacga gatccccggc tcgctggcgc tctccgagct
     1681 cgacggcatg aaggtcctcg acgtcgccgg cgcggaggtg ggtgagctcg ccgacgtcgt
     1741 cgcgagcacc gcgaccgacc gtccggtcgt gaccgcgttc ttcatcgacc gcgatgaggg
     1801 gcagctcgcg gcatcgtggg cgcaggtcgg cgagatcgat gtcgacggcg agcggctggt
     1861 gctgggcgtg ccgctcgagg cggtcaaggc ggcgtcgctg cgcccggacg agctccccct
     1921 ggtggacgcg ctgctcgaca accaggtgct ggacatgcgc cggcgggcgt tcgtgcgcgt
     1981 gcaggacgtg gtgctcgcgc cggacgcgga ccacctcgtg gtcgccggcg tcgacgccag
     2041 ctccgcggcg ctggcccggc gcttcggcct gggcttcatc tcgcgccgcc tgccgaagaa
     2101 gagcggcgac ttcgtcccct ggaacgacgt caacctgatc gcgctgcgcc tgtcgcggct
     2161 caacttcgtg gagtccttcg ccgagctcgc ggagctgcac ccggccgaca tcgccgaggt
     2221 catcagccag gtcggcacgc gtgaacgcgc ggcggtcctc gcggccctca acgccagcct
     2281 cgccgcggac acgctgcagg agatggaaga gggcctgcgc agcgcggcgc tgcaggagat
     2341 gccgctggag cgcgccgcac aggtcctcga gcacatcgag ccggacgagg ccgcggacgt
     2401 cctgagtcac cttccggaca cgctggcgca ggagctcctc gcgcgcctcc cggacaagcg
     2461 cgagcaggac ctgcgccgcc tcgccagcca ccccgagcac acggcggggt cgctcatgac
     2521 caccgacttc gtgatgctgc cgcgca
//
LOCUS       DASUMX010000130         4216 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_182252, whole genome shotgun sequence.
ACCESSION   DASUMX010000130 DASUMX010000000
VERSION     DASUMX010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4216
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182252"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1631)
                     /gene="lon"
                     /locus_tag="VFZ86_06385"
     CDS             complement(<1..1631)
                     /gene="lon"
                     /locus_tag="VFZ86_06385"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEX5641955.1"
                     /translation="MSEPQEPQVEEAAAQDIPAVLPVLPLRDTVVFPDTMIPLTIGQE
                     RSIKLIDDVLAGDRLLAMVTSKDAEIETPGPDLLYPVGTVGLAHKMIKLPDGTMRILV
                     QGLQRVRVEEYVSEEPYLVARTEKIEDVATESQESEALRASLLNVFSKIVALVPYLPE
                     ELEMAAANVEGPGPLTFLIASTMRIKAEDKQALLEEDDVEARMRKLIGILTRELDVLE
                     LGSKIQSDVRGEIDKSQREYFLRQQLKAIQQELGETDEQEAEINELRQKVEELALPEE
                     ADKAARRELDRLANISPQSAEYPVIRTYLDWIITLPWNVHSEDNLDVAHAREILDRDH
                     YDLDKVKDRILEFLAVRKLKDDLHGPILCFVGPPGVGKTSLGHSIAEAMGRKFERISV
                     GGVRDEAEIRGHRRTYVGAMPGIIIRAMRDAGTDNPLFMIDEIDKMGADWRGDPSSAM
                     LEVLDPEQNVTFRDHYLDLPFDLSKVLFITTANQLEPVPGPLRDRMEIINLAGYTIEE
                     KLHIAKKYLVPRQIEANGLRPGQVKFQDQAIVEIIS"
     gene            complement(1628..2059)
                     /locus_tag="VFZ86_06390"
     CDS             complement(1628..2059)
                     /locus_tag="VFZ86_06390"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX5641956.1"
                     /translation="MDVEKLHNEVLRAFNDSCVIATRQYSGRGFRPAADAYFEEDTRE
                     IVVRFDLAGMAMSDIELLVDRRELVVRGERTFQTREGRVYQQVEMDYGPFERRVRLMV
                     DVDPEVTTATYEAGVLEVRLTLTPQEQGARKIDIQTGGGDA"
     gene            2253..4172
                     /locus_tag="VFZ86_06395"
     CDS             2253..4172
                     /locus_tag="VFZ86_06395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HEX5641957.1"
                     /translation="MQLAFTTVDEFQSLLEQAGEALDYREVWPRLFPVANCPPELMRK
                     LVADIVESDERFLWESDVHVGLAAWRARRRDLADVAFTVVDIETTGATPGFCKITEIG
                     AVRVEGGREVATFSALVNPGMPIPAMITGITGIDDETVSGAPPIEVVLPKFVDFAAHS
                     VLVAHNAPFDLGFLDYELGKQRGQTFPRPALDTLRLARKLCPQQRCSLSALSYRFDTR
                     VKPVHRALHDAQATAELLLLFLSWLEEQGMHTLEEVARFCEPATRRNYHKISLTEKLP
                     TASGVYLMKDARGAPLYIGKAENLRRRTRDHFLQRQAFHARQALELLERIDVIETGSE
                     FAALLLESRLIAKHRPPYNSHGTRAAGYQYVKLSAEEYPRLYATPNLRDDGSYYAGPF
                     RKASLARRFVDCVNSAYPLRTCAHLPRGGREHACQRAGTGACLSPCSRPLNGEYAAVV
                     ADVRRVLEGHADDLDQRLVERQAALVQTLAFEQAARLQNQRETLARAARGVRRLRAAQ
                     REDLVLVYPSRRRGWAALWGVRAGSVVVEREVGRGAFTEQAARELLGEFAAAEPPRAP
                     LPAKAIDELLLVHSWVDAHRAAPNVVGLRAFVDGGEDAASAAARLLAAVRLAASGEPE
                     DTGPAPEAPAGATAL"
BASE COUNT          560 a         1542 c         1447 g          667 t
ORIGIN      
        1 gagatgatct cgacgatggc ctggtcctgg aacttgacct gccccggccg caggccgttg
       61 gcctcgatct gccgcggaac caggtacttc ttcgcgatgt ggagcttctc ctcgatggtg
      121 tagccggcga ggttgatgat ctccatgcgg tcgcgcagcg gacccgggac cggctccagc
      181 tggttggccg tggtgatgaa gaggaccttg ctgaggtcga acggcaggtc caggtagtgg
      241 tcgcggaacg tgacgttctg ctccgggtcg agcacctcga gcatcgcgct cgaggggtcg
      301 ccgcgccagt cggcgcccat cttgtcgatc tcgtcgatca tgaacagcgg gttgtcggtg
      361 ccggcgtccc gcatggcgcg gatgatgatg cccggcatcg cccccacgta ggtgcggcga
      421 tgcccgcgga tctcggcctc gtcgcgcacg ccgccgacgc tgatgcgctc gaacttgcgc
      481 cccatcgcct cggcgatgct gtggccgagg ctcgtcttgc ccacgccggg cgggccgacg
      541 aagcacagga tcggcccgtg caggtcgtcc ttgagcttgc gcaccgccag gaactcgagg
      601 atgcggtcct tgaccttgtc gaggtcgtag tggtcgcggt cgaggatctc gcgggcgtgc
      661 gccacgtcga ggttgtcctc gctgtgcacg ttccagggca gggtgatgat ccagtccaga
      721 tacgtgcgga tgaccgggta ctcggcgctc tgcggcgaga tgttggcgag gcggtcgagc
      781 tcccggcggg ccgccttgtc ggcctcctcc gggagcgcca gctcctcgac cttctggcgc
      841 agctcgttga tctccgcctc ttgctcgtcg gtctcgccca gctcctgctg gatggccttg
      901 agctgctggc gcaggaagta ctcgcgctgg ctcttgtcga tctccccgcg cacgtcgctc
      961 tggatcttgg agcccagctc cagcacgtcg agctcgcgcg tgaggatgcc gatgagcttg
     1021 cgcatccgcg cctcgacgtc gtcctcctcg agcagcgcct gcttgtcctc cgccttgatg
     1081 cgcatggtgc tggcgatgag gaacgtgagc ggcccggggc cttcgacgtt ggccgcggcc
     1141 atctcgagct cttccggcag gtacggcacc agcgcgacga tcttgctgaa gacgttcagc
     1201 aggctggcgc gcagggcctc cgactcctgg gactcggtgg cgacgtcttc gatcttctcg
     1261 gtgcgcgcga ccaggtacgg ctcctcgctc acgtactcct cgacgcgcac ccgctgcagg
     1321 ccctgcacca ggatgcgcat ggtgccgtcc ggcagcttga tcatcttgtg cgcgagcccg
     1381 accgtgccga ccgggtagag caggtccggc cccggcgtct cgatctcggc gtccttgctc
     1441 gtcaccatcg ccagcaggcg gtcgccggcg agcacgtcgt cgatcagctt gatgctgcgc
     1501 tcctggccga tggtcagcgg gatcatcgtg tccgggaaca ccaccgtgtc gcgcagcggc
     1561 agcaccggca ggacggcggg gatgtcctga gcggcggctt cttcgacctg cggttcctgg
     1621 ggctcgctca tgcgtcacct cctcctgtct ggatgtcgat cttgcgggcg ccctgctctt
     1681 gcggcgtcag ggtcagcctg acctcgagga cgccggcctc gtacgtcgcg gtggtgacct
     1741 ccgggtcgac gtcgaccatg aggcgcacgc ggcgctcgaa cgggccgtag tccatctcga
     1801 cctgctggta gacgcggccc tccctcgtct ggaacgtgcg ctcgccgcgc acgacgagct
     1861 cgcgccggtc gacgagcagc tcgatgtcgc tcatcgccat gccggccagg tcgaagcgca
     1921 ccacgatctc gcgcgtgtcc tcctcgaagt acgcgtccgc ggccggacgg aagccgcggc
     1981 cgctgtactg cctggtggcg atcacgcacg aatcgttgaa ggcgcgcagg acctcgttgt
     2041 gaagcttctc aacatccacg gggtgactcc tcagcgcggg gccccggccg ggcggcggat
     2101 gccgcggcgc cggggcacgt cttccggaaa actcggtcag ggacctaccc ggttcgctcg
     2161 cgagcgtaac gacgcgcccg ggacgagcgc aagacgggcg ccgcgtcatc gccgcccgtg
     2221 catccgtcgg cgcgggagcg taggattatc gcgtgcagct ggcgttcacc accgtcgacg
     2281 agttccagag cctgctcgag caggccggcg aagccctcga ctaccgcgag gtgtggcccc
     2341 gcctcttccc cgtcgccaac tgccctcccg agctcatgcg caagctcgtc gccgacatcg
     2401 tcgagagcga cgagcgcttc ctgtgggaga gcgacgtcca cgtcgggctc gcggcgtggc
     2461 gggcgcggcg ccgcgacctc gccgacgtgg ccttcaccgt cgtcgacatc gagaccacgg
     2521 gggccacgcc gggcttctgc aagatcaccg agatcggcgc cgtgcgcgtc gagggcgggc
     2581 gcgaggtcgc cacgttctcc gccctcgtca acccgggcat gccgatcccc gccatgatca
     2641 ccggcatcac cggcatcgac gacgagaccg tctccggggc gccgcccatc gaggtcgtgc
     2701 tgcccaagtt cgtcgacttc gccgcccact cggtgctggt ggcccacaac gccccgttcg
     2761 acctcggctt cctcgactac gagctcggca agcagcgcgg gcagacgttc ccgcggcccg
     2821 cactcgacac gctgcgcctg gcgcgcaagc tctgcccgca gcagcgctgc tcgctcagcg
     2881 ccctctccta ccgcttcgac accagggtca agccggtcca tcgcgccctg cacgacgcgc
     2941 aggccaccgc cgagctgctg ctgttgttcc tctcgtggct ggaggagcag ggcatgcaca
     3001 cgctcgagga ggtcgcgcgc ttctgcgagc ccgcgacccg gcgcaactac cacaagatct
     3061 cactcaccga gaagctcccg accgcctccg gcgtgtacct gatgaaggac gccaggggcg
     3121 ccccgctgta catcggcaag gcggagaacc tgcggcggcg cacgcgcgac cacttcctgc
     3181 agcggcaggc gttccacgcc cgccaggcgc tggagctgct cgagcgcatc gacgtgatcg
     3241 agaccggctc cgagttcgct gcgctcctgc tcgagagccg cctgatcgcg aagcaccggc
     3301 cgccgtacaa ctcgcacggc accagggccg ccggctacca gtacgtcaag ctcagcgcgg
     3361 aggagtaccc gcggctctac gccacgccca acctccgcga cgacggctcc tactacgccg
     3421 ggccgttccg caaggccagc ctggcccggc gcttcgtcga ctgcgtcaac agcgcctacc
     3481 cgctgcgcac ctgcgcccac ctgccgaggg gcgggcgcga gcacgcctgc cagcgcgccg
     3541 gcacgggcgc ctgcctgtcg ccgtgcagtc ggccgctcaa cggcgagtac gcggctgtcg
     3601 tcgccgacgt ccgccgcgta ctggaggggc acgccgacga cctcgaccaa cgcctcgtcg
     3661 agcgccaggc ggcgctcgtc cagacgctcg cgttcgagca ggcggcgcgc ctgcagaacc
     3721 agcgcgagac gctggcgcgc gcggcgcgcg gcgtgcgccg gctgcgcgcc gcgcagcgcg
     3781 aagacctcgt gctggtctac ccgtcgcggc ggcgcggatg ggcggcgctc tggggcgtcc
     3841 gcgccggctc cgtggtcgtc gagcgcgagg tgggccgcgg ggcgttcacc gagcaggcgg
     3901 cgcgggagct gctcggcgag ttcgccgccg ccgagccgcc gcgcgcgccg ctgccggcga
     3961 aggcgatcga cgagctgctg ctcgtgcact cctgggtcga cgcgcaccgg gccgcgccca
     4021 acgtggtcgg cctgcgcgcc ttcgtcgacg gcggcgagga cgcggcgtcc gccgccgccc
     4081 gcctgctcgc cgccgtccgg ctcgccgcct cgggcgagcc cgaggacacc ggtccggcgc
     4141 cggaggcgcc cgccggcgcc accgctctct gacgcaagaa aaggggcggc gggcgggccc
     4201 gtcaccaggc ccgccc
//
LOCUS       DASUMX010000131         3882 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_757989, whole genome shotgun sequence.
ACCESSION   DASUMX010000131 DASUMX010000000
VERSION     DASUMX010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3882
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_757989"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2113)
                     /locus_tag="VFZ86_06400"
     CDS             complement(<1..2113)
                     /locus_tag="VFZ86_06400"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HEX5641958.1"
                     /translation="MSAPHRIPESSPAGRAAAEALRRDVAGEVWTSPLRRWLYSTDAS
                     DYRIVPEVVVVAASPDDLAAVAAVSAETGLPVVARGAASSLAGQAIGPGIVVDCFRLD
                     RILAIDGDARTARVQPGVVQAALNAAAAPHSLEFGPDTSTVDQATIGGMVGNNSSGSR
                     SIVYGESKDKVVRVAGVLAGGESMTFGACAGGDLAAGARGTAGERLAAALARVRERYR
                     EPIATGFPQTRRCTSGYNLRELLAPEPNLGRLLAGSEGTLALFTELEVQLDPRPARRA
                     GAALTFATLRAGLEANLAILETAPSAVEFLDLAPLRRSPNVATFRRMAGLLAGAEEAM
                     LTVEYQGSEEEALAGLDRLRARAGELGAVQVVWLEQPADLAEAAALRRSVLPLLMGAP
                     GAERPAAFVEDTAVAPERLADFVADLQRVVAAHGARASFTGHASAGCLHLRPLLDLKT
                     AAGVARQEALASDVGRLVMEYHGAISGEHGCGRSRSWFLPQLLGPRLYEAMVAVKDAF
                     DPRRLLNPGVVVDGPPVSESLRFGPDYRGDHGWTPRLSYAAEGGFGPAVEKCFGAGLC
                     KKLTGTMCPPGIATRDEARTTRARANALQGVVCGAVPLGEIAADEFQEALGTCVACKA
                     CKTECPAGVDMAALKVEWLAEVRAREGVPPLARGIGDFRRLAALAAPVAPLVNGLGRT
                     RLARAVARQVGVARERPLPRFD"
     gene            complement(2163..2885)
                     /locus_tag="VFZ86_06405"
     CDS             complement(2163..2885)
                     /locus_tag="VFZ86_06405"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEX5641959.1"
                     /translation="MTAVEITSLSLTAIGLAFAAGLLSFVSPCVLPLLPVYLSFISGV
                     GVEDLGREHRRRLLLTSLLFVAGFTVIFVLMGAGAGGVGRLLIEYRDVLMIAAGAFIA
                     FSGLVVAGFIRLPEPVLKVAPKRAGAGGAFLTGAALAIGWTPCVGYVLGAILSMAATS
                     QSALSGSLLLLVYSMGLGVPFVLAALAFDWVSARLGWVKRHYKGIQVTSGLVLVVLGV
                     LMMFGILQQLSRWMPVVSPGGL"
     gene            complement(2928..3434)
                     /locus_tag="VFZ86_06410"
     CDS             complement(2928..3434)
                     /locus_tag="VFZ86_06410"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX5641960.1"
                     /translation="MKTRTIGIVIVVAVVVVAAVLAIASRDSSGGASPGGSSSGTSFA
                     GPIMAPDEFAVADFAGKPLVINMFGSWCPPCNAEAPDLATFAAENPGAQVVGIACEDT
                     EEGAVGFMEEFGLTYPLVMDDGTLVQEFGIPAYPTTIFFDAQGNEVDRLVGSATLDQF
                     NASLAKAQ"
     gene            3576..3779
                     /locus_tag="VFZ86_06415"
     CDS             3576..3779
                     /locus_tag="VFZ86_06415"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX5641961.1"
                     /translation="MASYDLVCDECGHDFEVFRQGFLKDEDRVCPDCGGTEVRQKFSS
                     FLSNLGGGGSGGGCAVPSGSPFG"
BASE COUNT          571 a         1441 c         1428 g          442 t
ORIGIN      
        1 ggtcgaacct tggcagcggc cgctcccggg ccacgccgac ctgcctcgcc accgcacgcg
       61 ccaggcgggt gcgcccaagc ccgttcacca gcggggcgac cggggcggcg agggccgcga
      121 gccggcgaaa gtcgcctatc cctcgtgcga gcggcggcac gccctcgcgc gcccggacct
      181 ccgcgagcca ctcgaccttg agcgccgcca tgtccacacc ggccgggcac tcggtcttgc
      241 acgccttgca ggcgacgcac gtgccgaggg cctcctggaa ctcgtccgcc gcgatctccc
      301 cgaggggcac ggcgccgcag accactccct gcagggcgtt ggcgcgcgcc cgcgtggtgc
      361 gcgcctcgtc gcgcgtcgcg atgcccggcg ggcacatcgt gccggtgagc ttcttgcaga
      421 gaccggcccc gaagcacttc tccacggccg ggccgaagcc gccctcggct gcataggaga
      481 ggcgcggcgt ccagccgtga tcgccgcggt agtcggggcc gaagcggagc gactccgaga
      541 ccggcggtcc gtccaccacg acgccgggat tgagcaggcg ccgcgggtcg aacgcgtcct
      601 tgacggccac cattgcctcg tagagccgcg ggccgaggag ctgcgggagg aaccagctcc
      661 gcgagcgccc gcagccgtgc tcgccggaga tcgcgccgtg gtactccatg acgaggcggc
      721 cgacgtcgct cgcgagcgcc tcctggcgcg ccactccggc ggcggtcttg aggtcgagca
      781 gcggccgcag gtgcaggcac ccggccgagg cgtgaccggt gaagctcgcc cgtgcgccgt
      841 gagcggcgac cacgcgctgc aggtcggcga cgaagtcggc gagacgctcc ggcgccaccg
      901 ccgtgtcttc cacgaaggcg gccgggcgct ccgcccccgg cgcccccatg agcagcggca
      961 ggacgctgcg gcgcagggcc gccgcctcgg cgaggtccgc cggctgctcg agccacacca
     1021 cctgcacggc gccgagctcg ccggcccgcg cccgcagccg gtccaggccg gcgagcgcct
     1081 cctcctcgct gccctggtac tcgacggtga gcatggcctc ctctgcgccg gcgagcaggc
     1141 cggccatgcg ccggaaggtc gcgacgttcg gggagcggcg cagcggcgcg aggtcgagga
     1201 actcgaccgc cgagggcgcc gtctcgagga tcgcgaggtt cgcctccagc cccgcgcgca
     1261 gggtggcgaa cgtgagcgcc gcgccggcgc gccgggcggg gcgcgggtcg agctgcacct
     1321 cgagctcggt gaagagcgcg agcgtgccct cggagccggc gagcagcctg cccaggttgg
     1381 gctccggcgc gagcagctcg cgcaggttgt agccggacgt gcagcggcgg gtctgcggga
     1441 agccggtcgc gatgggctcg cggtaccgct cgcgcacccg cgcgagcgcc gccgcgagcc
     1501 tctcccccgc cgtcccgcga gcgccggccg ccagatcgcc gccggcgcaa gccccgaacg
     1561 tcatcgactc gccgccggcg agcacgccgg cgacgcgcac gaccttgtcc ttgctctcgc
     1621 cgtacacgat ggaccgggag cccgacgagt tgttgccgac catgccgccg atggtggcct
     1681 ggtcgacggt ggaggtgtcg ggcccgaact ccagactgtg cggcgccgcc gccgcgttga
     1741 gcgccgcctg caccacgccc ggctggacgc gcgccgtccg cgcgtcgccg tcgatcgcca
     1801 ggatgcggtc gagcctgaag cagtcgacga cgatgcccgg cccgatcgcc tggccggcca
     1861 ggctggaggc ggcgccgcgg gcgacgacgg gcaggccggt ctcggcggag acggccgcca
     1921 ccgccgcgag gtcgtccggc gaggccgcca cgacgacgac ctcgggcacg atccggtagt
     1981 ccgacgcgtc cgtcgagtac agccagcgcc ggagcggcga ggtccacacc tcgccggcga
     2041 cgtcgcgccg cagcgcctcg gccgcggcgc gccccgccgg cgacgactca gggatgcgat
     2101 gaggagcgct cacgccgcca aggataccgc cggcacggcc ggcgcggcac gccccccggc
     2161 gatcagagcc cgccggggct gaccaccggc atccagcgcg agagctgctg gaggatcccg
     2221 aacatcatca gcacgccgag aaccacgagg accaggccgg acgtcacctg gatgcccttg
     2281 tagtgccgct tcacccagcc gagtctcgcg ctgacccagt cgaacgcgag cgccgcgagc
     2341 acgaagggca cgcccaggcc catggagtag acgaggagga gcagggagcc cgagagcgcg
     2401 ctctggctgg tcgcggccat ggacaggatc gcgccgagca cgtagcccac gcacggcgtc
     2461 cagccgatgg cgagcgcggc cccggtgagg aacgcgccgc cggcgccggc ccgtttgggc
     2521 gccaccttca gcaccggctc ggggagcctg atgaacccgg ccacgacgag cccgctgaag
     2581 gcgatgaagg cgccggcggc gatcatcagc acgtcgcggt actcgatgag gagccggccg
     2641 acgccgccgg cgccggcgcc catgagcacg aagatcacgg tgaaccccgc gacgaagagc
     2701 agcgaggtca gcagcaggcg gcggcgatgc tcgcgcccga ggtcctcgac acccacgccg
     2761 gagatgaacg agaggtagac cgggagcagc ggcagcacgc agggcgagac gaaggagagc
     2821 aggccggcgg cgaaggcgag accgatcgcc gtcagactga gcgaggtgat ctcgaccgcg
     2881 gtcatcggcg gcgccggggt cgggccgtca gcgcgcggac gggcgcgtca ctgcgccttc
     2941 gccagcgagg cgttgaactg gtcgagggtg gcggagccca cgaggcggtc cacctcgttc
     3001 ccctgcgcgt cgaagaagat cgtcgtcgga taggccggga tgccgaactc ctgcaccagg
     3061 gtcccgtcgt ccatcacgag cggataggtg aggccgaact cctccatgaa gccgaccgcg
     3121 ccctcctccg tgtcctcgca ggcgatcccc acgacctgcg cgcccgggtt ctccgccgcg
     3181 aacgtcgcga ggtcgggggc ttcggcgttg cagggaggac accaggagcc gaacatattg
     3241 atcacgagcg gcttgcccgc gaagtcggcg accgcgaact cgtccggcgc catgatcggg
     3301 ccggcgaagg aagtacccga ggacgaaccg cccggggagg ccccgcccga ggagtcgcgc
     3361 gaggcgatcg ccagcacggc ggccacgacc accacggcga cgacgatcac gatcccgatg
     3421 gtcctggtct tcatcctgcc cctcctcgtc cgcccgctgc accggtggcg gcggccgcgt
     3481 ctccagttta ctctgccccc acagtgtttg cgcaccggcg agcctcgcgt ataatacccc
     3541 ctagggtatg tagtccacca ccgcgatgga gggtcatggc gtcctacgat ctggtctgcg
     3601 acgagtgcgg gcacgacttc gaggtcttcc ggcagggttt cctgaaggac gaggaccggg
     3661 tctgtcccga ctgcggcggc accgaggtgc ggcagaagtt ctccagcttc ctcagcaatc
     3721 tcgggggcgg cggctccggc ggcgggtgcg ccgtcccgag cggcagcccc ttcggctgag
     3781 gctgaaccca ggcggcgcgg tcgcgccgcg aaggacagcc tcggcgccgg acccctccgg
     3841 cgcctgagac tcgaggggcg gggcggccac gaggccgccc cg
//
LOCUS       DASUMX010000132         3174 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1189698, whole genome shotgun sequence.
ACCESSION   DASUMX010000132 DASUMX010000000
VERSION     DASUMX010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1189698"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            192..1079
                     /locus_tag="VFZ86_06420"
     CDS             192..1079
                     /locus_tag="VFZ86_06420"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX5641962.1"
                     /translation="MASEYPGELGEACRATLAAGGKRVRPLLTLLCARSGAAAGEPVL
                     RAAAAVELLHMATLVHDDVLDRAELRRGRPTVAHQYGPATAVSAGNYLLARAFSELVG
                     AGDPSAVDLLSATAVGLSEGEVLQRAEAHDVGIGVEDYVRRCERKTADLFSAACSLGA
                     LLSGVEETAGAVGEYGRLIGLAFQVFDDILDCSGDEAATGKRPGADVRDGTITLPLIY
                     ALEARPELAEILRRPGASDADVETVLTAAAETGALERARGDALRYIEDARSVLAACPG
                     TVERGLLAQVAAQVVDRYS"
     gene            1098..2219
                     /gene="mqnE"
                     /locus_tag="VFZ86_06425"
     CDS             1098..2219
                     /gene="mqnE"
                     /locus_tag="VFZ86_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HEX5641963.1"
                     /translation="MNPTEISALAARSGLDPVWRKVEAGERLSDDDATALLSSSDILA
                     VGAMADFARARSAGDEVYFISNRHINHTNVCRNRCLFCAFSHDEGDADAYTLSVDEVV
                     EKARETLAVGGISEIHIVGGEHPDLPFSYYLDMMRALRELAPDVHIQAFTASEIAHFA
                     RISDQSVSEVLRELRDAGLGSLPGGGAEVFSGRVRDMICERKISGQTWLDVHRAAHEV
                     GMKSNATMLYGHVEEPAELADHMVRLRELQDDTGGFNAFIPLSFQPANTGLSELPGPT
                     GFDDLRMLAVGRLVLDNFRHVKAFWINVGLKLAQVSLAFGVNDLDGTVVEEKISHAAG
                     VDTGQELSKAELVHVIKAAGRVPVERDTLYNVVRRYDGA"
     gene            2209..3078
                     /locus_tag="VFZ86_06430"
     CDS             2209..3078
                     /locus_tag="VFZ86_06430"
                     /inference="COORDINATES: protein motif:HMM:NF014658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HEX5641964.1"
                     /translation="MAPEPPAAGAPADAPVRVGRIEFVNCFPLYHHFERELAARGVGA
                     AIVEGYPAALNEMLVDGAIDVTLSSSIAFARHADQQVLLPQVSISSFGAVDSIQLFAR
                     TPLARIRRVALTEKSATSICLLKVLCREWGIEPELAPRRGPLSEVLEDYDALLLIGDE
                     ALHLLRVEAYPHHFDLGAEWRSVTGLPMVYAVCAARRDFVDGCPEAAAAVGDALLASR
                     DACAAAPGVTAAAAAQAYDFSQAYLMDYFDKLKFGFTSEYRAGLQEFYRRAAAIGELG
                     RVPDLEACMTGVC"
BASE COUNT          427 a         1153 c         1130 g          464 t
ORIGIN      
        1 gagcttgcct ccatcatggc cggcgcgggc ctcatagagg tcaggtatcg ccgcctcggc
       61 ttcggcgcgg tggcgctgca cgtcggtgag gtgccccgct gaccccgaag ccctcgctga
      121 gctggtcgtc cgccgcccag aagcggcgga tcgcgcgcgg cctcgacctc gtcgaggagc
      181 gcctcaccgc ggtggcgtcg gagtaccccg gcgagctggg ggaggcctgc cgcgcgacgc
      241 tcgctgcggg cggcaagcgc gtgcggccgc tgctcacgct gctctgtgct cgcagcggcg
      301 cggcggcggg tgagccggtg ctccgcgcgg cggccgcggt cgagctgctc cacatggcca
      361 cgctcgtcca cgacgacgtg ctcgaccgcg cggagttgcg ccgtggccgg ccgaccgtgg
      421 cgcaccagta cgggcccgcc accgccgtgt ccgccggcaa ctacctcctg gctcgcgcct
      481 tctcggagct ggtcggcgcg ggcgatccct ctgcggtcga tctcctgagc gccaccgccg
      541 tggggttgag cgagggcgag gtcctgcagc gggccgaggc gcacgacgtg ggcatcggcg
      601 tggaggacta cgtacggcgc tgcgagcgca agaccgcgga tctgttctcg gcggcgtgct
      661 cgctgggcgc cctgctctcg ggggtggagg agacggcagg cgccgtcggc gagtacggcc
      721 gcctgatcgg cctcgccttc caggtgttcg acgacatcct ggactgcagc ggcgacgagg
      781 cggcgaccgg taagcggccc ggcgccgacg tgcgggacgg cacgatcacc ctgccgctca
      841 tctacgctct cgaggcccgg ccggagctgg cggagatcct ccgccggccg ggcgcgtccg
      901 acgccgacgt cgagaccgtc ctgaccgccg cagccgagac cggggcgctc gagcgtgccc
      961 gcggcgacgc tttgcggtac attgaggacg ccaggtcggt cctcgccgcc tgccccggca
     1021 ccgtcgagcg cggcttgctc gcccaggtgg ccgcgcaggt ggtcgaccgc tacagctgac
     1081 gactccggag tcaccccgtg aacccgaccg agatctccgc ccttgccgcg cgttccggcc
     1141 tcgaccccgt ctggcgcaag gtcgaggcgg gtgagcgcct gagcgacgac gacgcgaccg
     1201 ccctgctctc gtccagcgac atcctcgccg tgggggcgat ggccgacttc gcccgcgcgc
     1261 gcagcgccgg cgatgaggtc tacttcatct ccaaccgcca catcaaccac accaacgtct
     1321 gccgcaaccg ctgcctgttc tgcgccttca gccacgacga gggcgacgcc gacgcctaca
     1381 cgctgtccgt cgacgaggtc gtcgagaagg cccgcgagac gctggccgtc ggcggcatca
     1441 gcgagatcca catcgtcggg ggcgagcacc ccgacctgcc gttcagctac tacctcgaca
     1501 tgatgcgcgc gctgcgggag ctggcgccgg acgtccacat ccaggcgttc acggccagcg
     1561 agatcgcgca cttcgcgcgc atcagcgacc agtcggtgag cgaggtgctg cgcgagctga
     1621 gggacgccgg cctcgggtcg ctgcccggcg gcggcgccga ggtcttcagc ggtcgcgtgc
     1681 gcgacatgat ctgcgagcgc aagatcagcg gccagacgtg gctcgacgtg caccgtgcgg
     1741 cgcacgaggt cgggatgaag tccaacgcga ccatgctgta cgggcacgtc gaggagccgg
     1801 cggagctcgc ggaccacatg gtccgcctgc gcgagctgca ggacgacacg ggcggcttca
     1861 acgccttcat cccgctctcg ttccagccgg ccaacaccgg gctcagcgag ttgcccggcc
     1921 ccaccggctt cgacgacctc aggatgctcg ccgtcggccg cctcgtgctc gacaacttcc
     1981 gccacgtcaa ggcgttctgg atcaacgtcg gcctcaagct ggcgcaggtc tcgctggcgt
     2041 tcggcgtcaa cgacctcgac ggcacggtgg tggaggagaa gatcagccac gcggcgggag
     2101 tcgacacggg ccaggagctc agcaaggccg agctggtaca cgtgatcaag gcggccggcc
     2161 gcgtccccgt ggagcgcgac acgctctaca acgtcgtgcg gcggtacgat ggcgcctgag
     2221 ccgcccgcgg caggcgcgcc ggccgacgct cccgtgcgcg tcgggcgcat cgagttcgtc
     2281 aactgcttcc cgctctatca ccacttcgag cgggaactcg ctgccagggg cgtcggcgcc
     2341 gcgatcgtcg agggctaccc cgcggcgctc aacgagatgc ttgtggacgg ggcgatcgat
     2401 gtgacgctgt cctcctcgat cgccttcgcg cgccacgccg accagcaggt gctgctgccg
     2461 caggtctcca tcagctcgtt cggcgccgtg gactccatcc agctcttcgc gcgcacgccc
     2521 cttgcgcgca tccgccgggt cgcgctgacg gagaagagcg cgacctccat ctgtctgctc
     2581 aaggtgctct gccgggagtg gggcatcgag cccgagctcg cgccccggcg cgggccgctc
     2641 tcggaggtcc tcgaggatta cgacgcgctc ctgctcatcg gcgacgaggc gctgcacctg
     2701 ctgcgcgtgg aggcgtaccc gcaccacttc gacctcggtg cggagtggag gagcgtcacc
     2761 ggcctgccca tggtctacgc ggtgtgcgcc gcgcgccggg acttcgtgga cgggtgccct
     2821 gaggcggccg cggccgtcgg cgacgcgctg ctcgcgtccc gcgacgcatg tgcggcggct
     2881 cccggcgtca cggccgccgc ggcggcgcag gcctacgact tcagccaggc gtacctcatg
     2941 gactacttcg acaagctgaa gttcggcttc acgtccgagt accgcgccgg cctgcaggag
     3001 ttctaccgtc gcgccgcagc catcggcgag ctcgggcgcg tgcccgacct cgaggcgtgc
     3061 atgacgggcg tgtgctaggg gccgatgagc tgcagcagcc tcaccttccg ccggcccatc
     3121 ccggacgact gggagcgtgt catggccgtg atgcccgcgt ggtggaacgg gcgc
//
LOCUS       DASUMX010000133         7077 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_2053517, whole genome shotgun sequence.
ACCESSION   DASUMX010000133 DASUMX010000000
VERSION     DASUMX010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 7077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7077
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2053517"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(197..889)
                     /locus_tag="VFZ86_06435"
     CDS             complement(197..889)
                     /locus_tag="VFZ86_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641965.1"
                     /translation="MAIDEIIDRLYGLPLAEFTAARNEAARELRKAGEREAAERVKAL
                     RKPTAAAGAVNRLVREQRGEVEQFLQAAVALRDAQFSGKGDLATATRQEHEALGRLLA
                     TGGEAVRQTLLAAAVDDDAARQLREARLERELEPRGFGTLLAHAPQAAVTPAGTTAPQ
                     PAPKSPEREAPKPDDRVARSRLGDAEAALTAAETEEREARRRWEVAQHDLERARAAVD
                     TAQRDLDRLHGR"
     gene            complement(927..1406)
                     /locus_tag="VFZ86_06440"
     CDS             complement(927..1406)
                     /locus_tag="VFZ86_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020551024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5641966.1"
                     /translation="MISVCLWFDDQAEEAAQLYTTVLRDGALGRVVRYDEVSSGPSGK
                     APGSVMTVEFEAEGQSFIALNGGPIFTLSEATSLVLYRDTQEEIDEVWSALTEGGQEQ
                     PCGWLKDRFGVSWQVVPSELPALMEAEDPAAARRVTEALFSMKKIDIDALRRAKAGE"
     gene            1655..3106
                     /locus_tag="VFZ86_06445"
     CDS             1655..3106
                     /locus_tag="VFZ86_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011531048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase"
                     /protein_id="HEX5641967.1"
                     /translation="MTDDHRPEMPDPDVADDAGRLEVTRRQLLVGGAALGLSLTAAGS
                     LLTKEALAGGQTGVGQVQPPGQTSYPQMGYPMPGDPRPDAPELAYRGPYHVGVRRWTI
                     TNPDQLNILEYSATNTDPRYDRPLPIQVWYPAVIPGKVPELTTYADTLGSGPGNPDRP
                     VIPFEFPGRALRGARPDRSGAPFPLLIVSHGYPGSDVLLTNLTENLASKGYVVVAISH
                     TDSTHADATLFASTMLNRPLDINFVLKTMGELGAPGSGSFLSGLVDADRTGLIGYSMG
                     GYGALIAAGAGITPGTVYWGAPGGKLSIFLAGSPEYAALLDPRVKAIVPLAPWGGGYG
                     VWNAAGLAALEVPALFVVGDQDQTAPYAGVKFIFENAVNSDRYRLLYHAGDHEVPANP
                     APPITFTRWREYVHYQEPALDNTRTNNVNQHFLTAFLGLHLKGDADLGSYLDVVESSD
                     ASNNYNNPGYPEGIWKGFKIWSAVGMEMKHLHP"
     gene            complement(3183..4016)
                     /locus_tag="VFZ86_06450"
     CDS             complement(3183..4016)
                     /locus_tag="VFZ86_06450"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX5641968.1"
                     /translation="MNCHDVRETLEGYIAGDLADREASEVARHLDACPACAAAYEETR
                     LLVAELKDLSGAFRPAPLDATAFVSPVASPERRPRRRSWRPAWVLAAAVLLVVLAGAV
                     AVATVPSVARTVPLPVGAQLNDLQRQNELMLEQTRAMEERVRTLEERIKSLTGDDVDV
                     VPTSEQEIPDEVTMAVQQVVIQFIKAQYAGDVERLKELSTPALDARIDERPEDYLRDN
                     AGTVSFAQMTDVGIDENGLYLTFVRLSDTKVFSDSQYQENFAVEKSGDRYLVEYMETD
                     A"
     gene            complement(4013..4579)
                     /locus_tag="VFZ86_06455"
     CDS             complement(4013..4579)
                     /locus_tag="VFZ86_06455"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5641969.1"
                     /translation="MERGRGMDGERQLVEAARRGDARAYAALVRMHQRSLYCTAYSIL
                     GSSWDANDAVQDALLQSWARVDTLRDSARFKPWLVRILVNTCYDTLSTRRRLVPVGEA
                     PLEDTRRSQAHEFVGTEAQLDLFRVVRELDDDHRVVVALRYFNDLKVDDIATLLDIPA
                     GTVKSRINRALARLAQQLGRATRLEVES"
     gene            4739..6685
                     /locus_tag="VFZ86_06460"
     CDS             4739..6685
                     /locus_tag="VFZ86_06460"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEX5641970.1"
                     /translation="MTAAPSPDPGTAGEATLPRLFAAWVAEHGPEPALSRRVDGVFTA
                     FTYDELDRRASVLALALAREAGVRRGDLVALISDNRPEWLVCSLAIHFLGAVDVPRAS
                     DTPAEIRDAILRHAEPVVVILEDPAQLPEVRDALPGLRAAVVIDAPEPHEAAAAADAA
                     DAACPVFTLAGLMALGEAAWPRSAGEVARRREEVAPRDLATIIYTSGTTGAPKGVALT
                     HANYELNLRELPELLGIEREPVLSLLQPWHALERQMQLMYLSKGCCIHYSSVGALRGD
                     LTAVRPVVMATVPELWVKLYKGVFLRIDGQKPARRRLARWLVARALTSARARRVLAGR
                     EPAVRPRGPVRRAASIPWALLRSAVLAPFHALADRLVFREVRAGLGGRLRFPCVGGGP
                     LPDRVDEFFDAAGMPLLEGYGMTEAIVVIAMRDPHHRVLRTTGQVIRGMEHRLAGEDG
                     RPCRPGDVGRLQVRGPNIMRGYYKDPERTAEVLGADGWFDTRDLALVHADGSLRVLGR
                     LDDTLVLTNGENVNAPYLENELCASEWIDRAVVVGEGKPYVAAFVVPSRPHLEELARS
                     LGLAPDDLAALASDERVVKHFRHLASAISGDARRFAPWERVHRVHLWLEEFRIGRELS
                     QTLKLRRQEFLRLYSCEVDELYAP"
     gene            complement(6723..>7077)
                     /locus_tag="VFZ86_06465"
     CDS             complement(6723..>7077)
                     /locus_tag="VFZ86_06465"
                     /inference="COORDINATES: protein motif:HMM:NF015686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEX5641971.1"
                     /translation="IDMIFAIDSVLTAVGMTSEVLLIIVAITIAIAVMMFYAAPVSDF
                     ISRHTSVKLLALAFLVLIGVMLILEGIGEPIERTTVYVAILFALGVEYLNFRRRRNLQ
                     RRRGDDHVLPGQEGV"
BASE COUNT          971 a         2564 c         2486 g         1056 t
ORIGIN      
        1 ggccagcagt gacggccggc ctcctccgcg cgaggccggc aggtcgccgg atcattatga
       61 caccagggac aggggtgcgg tcgcacggcc cacccctgtc gtgcgtacgg tcacgaccgt
      121 cggcgcggcc acgcatctga ccccgatggc tgcgcgctga acatcgccgc gatgggatat
      181 cgaccggtct ctgctttcag cggccgtgga ggcggtcgag gtcgcgctgg gctgtatcga
      241 ccgccgcacg tgccctctcg aggtcgtgtt gggctacctc ccagcgacgt cgggcctccc
      301 gctcctcggt ctcggccgcg gtgagcgccg cctctgcgtc cccgagcctg gagcgggcaa
      361 cccggtcgtc tggctttggc gcctcccgct cgggcgactt tggtgccggc tgcggcgcgg
      421 tcgtgccggc aggcgtaacg gccgcctgag gtgcgtgcgc gagcagggtc ccgaagccgc
      481 gaggttccag ttcgcgctcg agtcgcgcct cccgcagttg ccgtgccgcg tcatcgtcga
      541 ccgcggcggc gaggagcgtc tggcgcacgg cctcgccgcc ggtcgcgagc aggcgcccga
      601 gcgcttcgtg ctcctgcctg gtcgccgtgg ccaggtcgcc cttgccgctg aactgcgcgt
      661 cgcgcagcgc gacggctgct tgcaggaact gctcgacctc gccgcgctgc tcgcggacga
      721 gccggttcac cgcgccggcg gccgcggtcg gcttgcgcag cgctttcacc cgttcggccg
      781 cttcgcgttc tcccgccttg cgcagctcgc gcgcggcctc gttgcgcgct gcggtgaact
      841 ccgcgagcgg cagtccgtac agccggtcga tgatctcgtc gatggccatc ctcagggccg
      901 tccggcctgc tgccgcggca cggcgcctac tctccggcct tcgcgcgtcg cagggcgtcg
      961 atgtcgatct tcttcatcga gaacagcgct tcggtgacgc gcctggcggc ggccgggtcc
     1021 tccgcctcca tgagcgccgg cagctcgctc ggcacgacct gccaggagac cccgaagcgg
     1081 tccttgagcc agccgcacgg ctgctcctgc ccgccctcgg tgagcgcgga ccagacctcg
     1141 tcgatctcct cctgggtgtc gcggtagagg accagcgacg tcgcctcgct caaggtgaag
     1201 atcgggccgc cgttgagcgc gatgaagctc tgtccctcgg cctcgaactc gaccgtcatc
     1261 accgatccag gcgctttgcc cgacggcccc gaactgacct cgtcgtaccg gacgacccgc
     1321 ccgagcgctc cgtcgcgcag caccgtcgtg tacagctgcg cggcctcctc ggcctgatcg
     1381 tcgaaccaca gacatacgga aatcatgcgt actccatctt ccgctgatgt gccttcacac
     1441 cgaggcggta cccgggcatg ccgggcgcaa actccccgct tcggcgtgta gccagaagag
     1501 gcaacgcccg gcgacggctc cgcacattga cgaccgtgaa gtcgcctgct agaccggagg
     1561 cgtccgcccg ggcgcgcggc gagggtgact ctggccacgc gtgatcccgg gagtcacggt
     1621 cggcgatgcc ggcccgcgac gagggggtag cacgatgacc gacgaccacc gtcccgagat
     1681 gcctgatccc gacgtcgcgg acgatgccgg ccggctcgag gtcacccggc gccagttgtt
     1741 ggtgggcggg gcggcgctcg ggctctcgct gaccgccgcc ggctcgctgc tgaccaagga
     1801 ggcgctcgcc ggcggccaga cgggcgtggg tcaggtccag ccgccgggcc agacttccta
     1861 tccgcagatg gggtacccga tgcccggcga ccctcgtccc gacgcgccgg agctcgccta
     1921 ccgcggcccc tatcacgtgg gcgtccgtcg ctggacgatc acgaacccgg accagctgaa
     1981 catcctcgag tacagcgcca ccaacacgga cccgcgctac gaccggcctc tgccgatcca
     2041 ggtgtggtac ccggccgtca tccccgggaa ggtgcccgaa ctgaccacct atgcggacac
     2101 actggggagc ggacctggca acccggaccg cccggtgatc ccgttcgagt tccccgggcg
     2161 cgctctgcgc ggggcgcgtc cagaccgcag tggcgccccg ttcccgttgc tcatcgtctc
     2221 gcacgggtac ccgggctccg acgtgctcct caccaacctg acggagaacc tcgcctccaa
     2281 aggctacgtg gtcgtcgcca tctcgcacac cgactcgacg cacgccgacg cgaccctctt
     2341 cgccagcacc atgctgaacc gcccgctcga catcaacttc gtgctcaaga ccatgggcga
     2401 gttgggcgcg ccggggtcgg gcagcttcct gtccggcctc gtcgacgcgg acaggacggg
     2461 gctcatcggg tactcgatgg gcggctacgg cgcgctgatc gccgccggcg ccggcatcac
     2521 tccagggacg gtgtattggg gggcgcccgg cggaaagctc agcatcttcc tggcgggaag
     2581 ccccgagtac gccgccctgc tcgacccgcg cgtcaaggcg atcgtgcctc tggcgccgtg
     2641 gggcggcggc tacggagtct ggaacgcggc cgggctggcc gcgctcgagg tcccggcgct
     2701 gttcgttgtg ggtgatcagg accagacggc gccctacgcg ggcgtcaagt tcattttcga
     2761 gaacgcggtg aactccgacc gttaccggct cctgtaccac gccggggatc acgaggtgcc
     2821 ggcgaacccg gctccgccga tcaccttcac gcgctggcgc gagtacgtgc actaccagga
     2881 gccggcgctc gacaacacgc gtaccaacaa cgtcaaccag cacttcctca cggccttcct
     2941 cggcctgcac ctgaaggggg acgcggacct cggcagctac ctcgacgtgg tcgagagctc
     3001 ggacgccagc aacaactaca acaacccagg ctacccagag ggcatctgga agggcttcaa
     3061 gatctggtcc gccgtcggca tggagatgaa gcacctgcac ccgtgagacg cggctgagcg
     3121 cagggaaggg gggcggcccg gacgtcgtca gcggggtccg ggccgccgcg gtagagacgc
     3181 cgtcaggcgt cggtctccat gtactccacc aggtaccggt cgccggactt ctcgacggcg
     3241 aagttctcct ggtactgcga gtccgagaac accttggtgt cgctgaggcg cacgaaggtc
     3301 aggtagaggc cgttctcgtc gatgccgacg tcggtcatct gcgcgaaaga aacggtgccg
     3361 gcgttgtcgc gcaggtagtc ctcgggccgc tcgtcgatgc gcgcgtcgag cgccggcgtc
     3421 gacagctcct tcaggcgctc cacgtcaccg gcgtactgcg ccttgatgaa ctggatcacg
     3481 acctgctgca ccgccatcgt gacctcgtcc gggatctcct gctccgacgt cggcaccacg
     3541 tcgacgtcgt cgccggtgag gctcttgatg cgctcctcga gcgtgcgcac gcgctcctcc
     3601 atcgcacgcg tctgctcgag catgagctcg ttctgccgct ggaggtcgtt cagctgcgcg
     3661 cccaccggca gcggcaccgt gcgcgccacg gaggggaccg tcgccaccgc cacggcgccc
     3721 gcgaggacga cgaggaggac ggcggcggcc agcacccacg caggccgcca ggagcggcgg
     3781 cggggacgcc tctccggtga cgccacgggg ctcacgaagg cggtggcgtc cagcggcgcc
     3841 gggcggaagg cgccgctcag gtccttcagc tccgctacca gcagccgcgt ctcctcgtag
     3901 gcggcggcgc aggccgggca ggcgtcgagg tgcctggcca cctcgctggc ctcacgatcc
     3961 gcgagatcgc cggcgatgta gccctccagg gtctctctca catcgtggca gttcatgact
     4021 ccacctccag acgcgtcgcc cggccgagtt gttgcgcgag ccgtgccagg gcgcgattga
     4081 tgcgtgactt caccgtgccg gcggggatgt ccagcagggt cgcgatgtcg tccaccttca
     4141 ggtcgttgaa gtagcggagc gcgacgacga cgcggtgatc gtcgtcgagc tcgcggacca
     4201 cgcggaacag gtcgagctgg gcctcggtgc ccacgaactc gtgcgcctgg ctccgtctcg
     4261 tatcctcgag cggcgcctcg cccaccggca cgagacggcg cctcgtgctc agggtgtcgt
     4321 agcaggtgtt gaccaggatg cgcacgagcc agggcttgaa gcgcgcgctg tcgcgcagcg
     4381 tgtccacgcg cgcccaagac tgcagcaggg cgtcctgcac ggcgtcgttg gcgtcccagg
     4441 aggacccgag gatcgagtag gccgtgcagt agagcgagcg ctggtgcatc ctcaccagcg
     4501 cggcgtacgc gcgcgcatcg ccgcgccgcg ccgcctcgac gagctgccgt tcaccgtcca
     4561 tccctcgccc ccgttccatg gccttgtttg tacaggactc cgggggcggg cgaaacgttc
     4621 cgtagccgcg ggccggccgg ccgccgctac gacctctgtt gacagggggc ggcgctcatg
     4681 gacaatggcc gcaggccggc cgggggagag tggccggcag cacctgcgga gagggcgtat
     4741 gactgcagca ccgtcaccgg acccgggcac ggcaggggag gcgacgctgc cgcggctgtt
     4801 cgccgcctgg gtcgccgagc atgggccaga gccggccctc agccggcgcg tggacggcgt
     4861 cttcaccgcc ttcacctacg acgagctcga ccggcgcgcg agcgtgctgg cgctggccct
     4921 cgcgcgcgag gccggcgtgc gccgcggcga cctcgtcgcc ctcatcagcg acaaccggcc
     4981 cgagtggctg gtgtgcagcc tggcgatcca cttcctcggc gccgtggacg tgccgcgcgc
     5041 gtccgacacg ccggcggaga tccgggacgc catcctgcgg cacgcggagc ccgtcgtggt
     5101 gatcctggag gatcccgcgc agctgccgga ggtgcgcgat gccctgcccg ggctgcgggc
     5161 ggcggtggtc atcgatgccc cggagccgca tgaggccgcg gccgccgccg acgccgccga
     5221 cgccgcctgc cccgtgttca cgctggcggg gctgatggct ctcggtgaag cggcgtggcc
     5281 gcggtcggcc ggggaggtcg cgcggcgccg cgaggaggtg gcacccaggg atctggcgac
     5341 gatcatctac acctcgggca cgaccggcgc gcccaagggc gtggcgctca cccacgccaa
     5401 ctacgagctc aacctgcgcg agctccccga gttgctgggg atcgagcgcg agccggtgct
     5461 ctcgctgctg cagccgtggc acgctctgga gcgccagatg cagctcatgt acctgagcaa
     5521 gggttgctgc atccactaca gcagcgtcgg ggcgctgcgc ggcgacctga cggcggtccg
     5581 cccggtggtc atggcgaccg tcccggagct gtgggtcaag ctctacaagg gcgtcttcct
     5641 gaggatcgac gggcagaagc cggcgcggcg caggctggcg cgctggctgg tcgcccgcgc
     5701 gctgacgtcg gcgcgggcgc ggcgggtgct ggcgggccgc gagccggctg tgcggccgcg
     5761 cggcccggtc cgccgggcgg cctccatccc gtgggcgttg ctccggtcgg ccgtcctggc
     5821 gccgttccac gccctcgccg accgcctcgt gttccgcgag gtccgcgccg ggctcggcgg
     5881 ccggttgcgc ttcccctgcg tcggcggcgg cccgctgccg gacagggtcg acgagttctt
     5941 cgacgccgcc ggcatgccgc tgctcgaggg ctacggcatg acggaggcca tcgtcgtgat
     6001 cgcgatgcgc gacccgcacc accgggtgct gcgtaccacc ggccaggtga tccgcggcat
     6061 ggagcaccgc ctcgccggtg aggacggccg cccgtgccgg ccgggcgacg tcggccgcct
     6121 gcaggtccgc gggcccaaca tcatgcgcgg ctactacaag gatccggagc ggacggcgga
     6181 ggtgctcggc gcggacggct ggttcgacac ccgggacctc gcgctggtgc acgccgacgg
     6241 cagcctgcgc gtcctcggcc gcctcgacga cacgctggtg ctcaccaacg gcgagaacgt
     6301 caacgcgccg tacctggaga acgagctgtg cgccagcgag tggatcgacc gggcggtcgt
     6361 cgtcggcgag ggcaagccct acgtggccgc cttcgtggtc cccagccgcc cgcacctgga
     6421 ggagctcgcc cggagcctgg ggctggcccc ggacgacctg gcggcgctcg cctccgacga
     6481 gcgcgtcgtc aagcacttcc gccatctcgc cagcgcgatc agcggcgacg cccggcgctt
     6541 cgcgccctgg gagcgcgtgc accgcgtgca cctctggctg gaggagttcc gtatcggccg
     6601 cgagctctcc cagacgctca agctgcgacg gcaggagttc ctgcgcctct actcctgcga
     6661 ggtcgacgag ctgtacgcgc cctgagcgcg tcccctgagc ggtctcgtgg ccgcggccgc
     6721 gctcacacgc cctcctggcc cgggagcacg tggtcgtcgc cccggcgccg ctgcaggttc
     6781 cggcgccggc ggaagttcag gtactcgacg ccgagcgcga acaggatcgc cacgtacacg
     6841 gtggtgcgct cgatcggctc gccgatgccc tcgaggatca gcatcacgcc gatgaggacg
     6901 aggaaggcga gcgccagcag cttcacgctc gtgtggcggc tgatgaagtc cgacaccggc
     6961 gccgcgtaga acatcatcac ggcgatcgcg atggtgatgg cgacgatgat cagcagcacc
     7021 tcgctggtca tgccgaccgc cgtcagcacc gagtcgatgg cgaagatcat gtcgatg
//
LOCUS       DASUMX010000134         2953 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_902980, whole genome shotgun sequence.
ACCESSION   DASUMX010000134 DASUMX010000000
VERSION     DASUMX010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_902980"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VFZ86_06470"
     CDS             <1..216
                     /locus_tag="VFZ86_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015103449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5641972.1"
                     /translation="LDSRTGGEVLDLLRRSVDEFGQTVIMVTHDANAASIADRVVFLK
                     DGRIELDEDRMTRDAIYDTIKNLEVTR"
     gene            213..2756
                     /locus_tag="VFZ86_06475"
     CDS             213..2756
                     /locus_tag="VFZ86_06475"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX5641973.1"
                     /translation="MTRLAFRNLASRPLRTVLTLLAILLGVAMITGTYVLTDQIDNGF
                     ANIFETSFKGTAVQVEPKTSFTSMEGDAPQTLSDGLVAEVLAVPGVDKAAGIYETVGA
                     AIVDGKPVETGGAPTLLTTSTGEPFNQATLVAGGEIQGRNEVAIIKSFADKAGLRPGD
                     SFTVAAPAGLEEVKVAGVFEWGEAGSMGGTIVVAGRLQDVQRWGGEPGRISGISVSAD
                     PGVTPQELAADVKAAVPAGVSVKTGEQAAADATAETADAIGSFLTPVLLAFAGVSVFV
                     GAFIIFNAFSITVAQRRREFAMLRALGASRRQVLSSVVAEALTLGVLASAIGIGAGLG
                     VAKGINLLFKALGADIPTAGIALEPRTILIAVLVGVGVTLLSALAPALRATRVPPVAA
                     LQEGATLPPTRFTRFSTVLALAVTALGAGALAFGIFSDGGTNGRLLFMGLGGLLLFVA
                     IAMLAKFIVRPASRIIGWPMEKLAPTAGRLARDNAGRNPSRTAATAAALMIGLAMVVF
                     VAVFAQAMKGSFSDAISKSTRADLVAQDQSAFLTVPQKTVRGLEDVPGVESAAGVAWA
                     ALKIKGGGVITANAVDPAVWPEVWGHDWRRGGSDELFAKLDADSVILEDGSPIAAKVK
                     AGDTISVTSQSGEKAELTVLGFYRDQMAYTGMMVSLDALEKLDVPTAAGMMLVRSDGS
                     EGTQAAVQKALAEFPTQKVQTKTEYLDAINSQVDQILMMFYGLLAMSVIISIFGIVNT
                     LVLSVHERTREIGMLRAIGATRRQLGQMVRYESVITAVIGGVLGTLVGIAMGYVIVTQ
                     LGGDGLVFSLPWTQLGVFLVLSVVVGVVAAVLPARRAAGTRILEAIQYE"
BASE COUNT          429 a         1097 c         1002 g          425 t
ORIGIN      
        1 ctcgactcgc gcaccggcgg cgaggtcctc gacctgctcc gccgcagtgt cgacgagttc
       61 ggccagaccg tgatcatggt cacgcacgac gccaatgccg ccagcatcgc cgaccgcgtc
      121 gtcttcctca aggacggccg catcgagctg gacgaggacc gcatgacccg cgacgccatc
      181 tacgacacca tcaagaacct cgaggtgacc cgatgacccg cctcgccttc cgcaacctgg
      241 ccagccggcc actccggacg gtcctcacgc tgctcgcgat cctgctgggc gtggcgatga
      301 tcaccggcac gtacgtgctg accgaccaga tcgacaacgg gttcgccaac atcttcgaga
      361 cctcgttcaa gggcacggcc gtgcaggtcg agcccaagac ctcgttcacc agcatggagg
      421 gcgacgcgcc ccagaccctg agtgacggcc tcgtcgccga ggtgctcgcc gtcccgggcg
      481 tcgacaaggc cgccggcatc tacgagacgg tgggcgccgc catcgtcgac ggcaagcccg
      541 tcgagaccgg cggcgccccc accctgctca cgaccagcac gggtgagccc ttcaaccagg
      601 cgaccctcgt cgccggcggc gagatccagg gccgcaacga ggtcgccatc atcaagagct
      661 tcgccgacaa ggccggcctc cggcccggcg acagcttcac cgtcgccgcc ccggccggcc
      721 tcgaagaggt caaggtcgcc ggcgtgttcg agtggggcga ggccggctcc atgggcggca
      781 ccatcgtcgt cgccggccgc ctgcaggacg tgcagcgctg gggcggcgag cccggcagga
      841 tcagcggcat ctccgtgtcc gccgaccccg gcgtgacgcc ccaggagctc gccgcggacg
      901 tcaaggccgc cgtgccggcc ggcgtcagcg tcaagaccgg cgagcaggca gccgcggacg
      961 cgaccgccga gacggccgac gcgatcggct cgttcctcac gccggttctc ctggcgtttg
     1021 ccggcgtctc cgtcttcgtc ggcgcgttca tcatcttcaa cgccttcagc atcaccgtgg
     1081 cccagcggcg ccgcgagttc gcgatgctgc gcgcgctcgg cgccagccgc cgccaggtgc
     1141 tctcgtccgt ggtcgccgag gcgctcacgc tcggcgtgct ggcctcggcc atcggcatcg
     1201 gcgccggcct cggcgtcgcc aagggaatca atctgctctt caaggccctc ggcgccgaca
     1261 tccccacggc cggcatcgcg ctcgagccgc ggacgatcct catcgccgtg ctcgtcggcg
     1321 tgggcgtcac ccttctctcg gcgctggcgc cggccctgcg cgccacgcgc gtgccgccgg
     1381 tggcggccct gcaggagggc gcgaccctgc caccgacgcg gttcacccgg ttcagcacgg
     1441 tgctcgccct ggccgtcacg gccctcggcg ccggcgcgct cgccttcggc atcttcagcg
     1501 acggcgggac caacggccgc ctgctgttca tgggtctcgg cggcctgctg ctcttcgtcg
     1561 ccatcgccat gctggccaag ttcatcgtcc ggccggcgag ccgcatcatc ggctggccga
     1621 tggagaagct cgcccccacc gccggccggc tcgcccgcga caacgccggc cgcaacccga
     1681 gccgcacggc cgccacggcc gccgcgctca tgatcggcct cgccatggtc gtcttcgtcg
     1741 ccgtgttcgc ccaggccatg aagggctcgt tcagcgacgc gatcagcaag tccacgcgcg
     1801 ccgacctcgt ggcgcaggat cagagcgcgt tcctcaccgt gccccagaag acggtgcgcg
     1861 gcctcgagga cgtgcccggc gtcgagtccg ccgccggtgt cgcctgggcg gcgctcaaga
     1921 tcaagggcgg cggcgtcatc accgccaacg ccgtggatcc cgcggtgtgg cccgaggtct
     1981 ggggccacga ctggcggcgc ggaggcagcg acgagctgtt cgccaagctc gacgcagaca
     2041 gcgtgatcct cgaggacggt tcgccgatcg ccgccaaggt caaggccggc gacacgatct
     2101 ccgtcaccag ccagagcggc gagaaagccg agctcacggt gctcggcttc taccgcgacc
     2161 agatggccta cacaggaatg atggtcagcc tggacgcgct cgagaagctg gacgttccga
     2221 cggcggccgg catgatgctg gtgcgcagcg acggcagcga ggggacccag gccgcggtcc
     2281 agaaggcgct cgcagagttc ccgacgcaga aggtccagac caagaccgag tacctcgacg
     2341 ccatcaacag tcaggtcgac cagatcctca tgatgttcta cggcctgctg gcgatgagcg
     2401 tgatcatctc gatcttcggc atcgtcaaca ccctggtgct gagcgtgcac gagcgcaccc
     2461 gcgagatcgg catgctgcgc gccatcggcg ccacgcgacg gcagctcggc cagatggtcc
     2521 gctacgagag cgtgatcacc gccgtgatcg gcggcgtgct cggaacgctc gtcggcatcg
     2581 ccatgggcta cgtgatcgtc acgcagctcg gcggggacgg gctggtcttc agtctgccgt
     2641 ggacgcagct gggcgtgttc ctggtgctgt ccgtggtggt cggagtggtc gcggcggtgc
     2701 tccctgcgcg gcgcgccgcc ggcacgcgca tcctggaggc catccagtac gagtagcctc
     2761 cggcaggacg gcgagacgtg gccggctcca tccccttggg ggccgccccg tccgtcagag
     2821 gggccggggc ggcgcaagcg ccaccccggc cctctctcgt gccgccgccc ccagggggag
     2881 gaagggcggt gggtcgccgg gctggcccgg cggtcgtcag ctccggatca ttcgtactgg
     2941 atggcctcca gga
//
LOCUS       DASUMX010000135         8669 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_617499, whole genome shotgun sequence.
ACCESSION   DASUMX010000135 DASUMX010000000
VERSION     DASUMX010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 8669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8669
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617499"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="VFZ86_06480"
     CDS             <1..181
                     /locus_tag="VFZ86_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006966891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEX5641974.1"
                     /translation="GMPILNAHDAGAVIVCNLDEKPGYSGVPNPLYDDPKAILLFGDA
                     KGTLGELISGLENAA"
     gene            401..2146
                     /locus_tag="VFZ86_06485"
     CDS             401..2146
                     /locus_tag="VFZ86_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HEX5641975.1"
                     /translation="MGDLLRIDLSTRTFSEETVPPQLIKELIGAKGIGTHYLCEEVGP
                     EVDPLSPDNKLIFVVGPMGGTTMLGSNRYAVYFASPLTGGYGECYSGGNLTPQFAKTG
                     YKVVIVEGKADAPIYLEVSEEGAAIHDASDLWGMDAFKAEDEILARTPHKKAQACVIG
                     QAGERLVRFACVNNNYWHQLGRGGPGAVFGSKNLKGIAWHGEKKVEVARPDDFKAVVR
                     DLIERTKDDPGAAAYQRGGTLNMVRIMNGVNAFPTRYWRKGTLENFERITVETMLAEH
                     GTVNQTCPPCVFKCVKRNTVFKGRHEGLVVEGPEYETAFVFGGLCEIDDFAEIMWLND
                     ICDRLGVDTMDAGNLCALAIECAERGLIDEKLTWNDPVGVGAFLEKICLREGIGDLFA
                     EGVVAVAGEYGLEDVAVHVKGMAPPGYEPRKMKGMGLGFATTPRGACHLRATMYKPEL
                     MGVTTYGEVDDKAPVYVDWEDRLTIMDTLVYCRFYRDMVQWPYITAVVNAVIGTDYTE
                     ADLHGIANRIITMTHDFNAARGIGRESEKLPDWVTEKPMEDEEGLTFPQEEMASMLQD
                     YYTLRGWGELPPLAG"
     gene            complement(2258..2608)
                     /locus_tag="VFZ86_06490"
     CDS             complement(2258..2608)
                     /locus_tag="VFZ86_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641976.1"
                     /translation="MPWYVLLIIALASLALVVAMLVRLGLIGWRLAKHGMAVSRRVAP
                     LADGLARRTDEITVAAERLSADAGQLGDNLARMQRSAARLQVVTQTFNDAMRPYYLIA
                     GWLSGEKEWSDLGI"
     gene            complement(2665..3768)
                     /locus_tag="VFZ86_06495"
     CDS             complement(2665..3768)
                     /locus_tag="VFZ86_06495"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HEX5641977.1"
                     /translation="MTGFEATGTWVQRSAAALAAACFAGVLALILLGAAVHPPAAPAT
                     PASVQDLEAQARGVRREVSRLDHRAGILTEKYNLARAELDVLNVRLLETRRDLERAQV
                     ELETAQALRGARLAAMYKSDGYGVLDVLFSLSDLGEADTQLGYFRSIDEADQETVTRI
                     AAMETRIQALATQTDADRAEALDKEMALREQQAAIEDQLAARETLLADLDARVKKLLA
                     QQAKLDAAASARLARAAGVDLATIHGAPAQIAVVRETMKYLGIPYVWAGATPSGGFDC
                     SGLVLYVYAKFGVVFPHGATMQAHMGKPVSLAQAQPADLVFFGTPAFYHHVGIYIGNG
                     LFIEAPHTGDVVKVSRLAGRGCSLICRYSLSLP"
     gene            3884..3960
                     /locus_tag="VFZ86_06500"
     tRNA            3884..3960
                     /locus_tag="VFZ86_06500"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3918..3920,aa:Met,seq:cat)
     gene            4079..4819
                     /gene="aqpZ"
                     /locus_tag="VFZ86_06505"
     CDS             4079..4819
                     /gene="aqpZ"
                     /locus_tag="VFZ86_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004100621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HEX5641978.1"
                     /translation="MNTGRAAVAEFFGTFWLVFGGTGAAVLAAAVGDNGIGWLGVSLA
                     FGLTVVTMAYAVGHISGGHFNPAVTVGLWAGGRFPGGKVAVYIAAQVVGAVAASAVLY
                     VIASGRAGFTLADGFAANGYGDHSPAGYSLAAAIVIELLLTFVFLLVIHGVTDRRAPA
                     GLAPLAIGLTLALIHLISIPVTNTSVNPARSLAPALFVGDWALAQLWVFILFPTIGGI
                     IGGLAYRYLLDAPASERDKVGGPGEATE"
     gene            5105..5227
                     /locus_tag="VFZ86_06510"
     CDS             5105..5227
                     /locus_tag="VFZ86_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5641979.1"
                     /translation="MSVLLVVNAPARGAEGSLYAFRHADATTGSAAGDDRVVPA"
     gene            5236..6672
                     /gene="tilS"
                     /locus_tag="VFZ86_06515"
     CDS             5236..6672
                     /gene="tilS"
                     /locus_tag="VFZ86_06515"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEX5641980.1"
                     /translation="MARLPAACLRLQRSVAESIAATRALRPGEHALLLLSGGADSMAL
                     LSLVRAADRRLGLGLRLATLHVDYGLRGADSDRDRLIVERACAGAGLPFHVERLHGSL
                     NGRDFQARARAQRYGRARQLTAEHGYDVIVTAHNRDDQAETVVYRLAKYASPRGLAGM
                     RPRDRDLARPLLGAGAVELRAYCRAAGIEYGEDATNAEPRYARNLLRLEVLPLLEALN
                     PRLAETLAAGAEQAAAEAQVLGEAAAEARARVERPRGRGDHAAVDLTGLAAEPAALRA
                     LLLHELLREAMGGDALVERRLVEAVLRLTERADDAGRVSLGRGLEAVRGGGVLRIRPV
                     AAGHVCAPLVLEGVGLAAAGEAGVTARFCGGRWRVRLLPGAALDRAAAIAGEGFAGLA
                     GPPRRVTLRHPRRGERFAPLGLGGETTVARHLAAARVPAERRPLAVVLDVDGRAAWVG
                     GAAPARVAQPFRVAQSSVLTLHVVQEGT"
     gene            6669..7235
                     /gene="hpt"
                     /locus_tag="VFZ86_06520"
     CDS             6669..7235
                     /gene="hpt"
                     /locus_tag="VFZ86_06520"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEX5641981.1"
                     /translation="MNVPIAEVLVDREALATRVDELAVQITADYAGKELLLVGVLKGA
                     VFFMADLARRIDLPVALDFMAVSSYGSSTDSSGVVRILKDLDLEIADRHVLIVEDIID
                     SGLTLSYLLKNLRSRKPASLEICALLTKPSRRRTEIPCRYVGFEIPDKFVVGYGLDLA
                     DRFRTLDYIGVLTPEAAAGIVDQAGSLL"
     gene            7332..>8669
                     /locus_tag="VFZ86_06525"
     CDS             7332..>8669
                     /locus_tag="VFZ86_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5641982.1"
                     /translation="MSRFIRSALFPILIVIIVAMFIEWVISGNRANEAPKAIYSAPFV
                     TTQTVLTSDLQNGLVKNVILDPTSGAAKITQTGGLQYYVEDITDAQALALEEQIGSDQ
                     PEVVVIKGPVAPPAAKDVQPSFTNDVINGRVDAVVMNVKDQTMEVTLQPVDGKAQQYT
                     VAYTDPAATTAFLDQNNVPYDATSPKSAWWTSALFTILPILLILGFWFFMMNQMQGGG
                     SKVMSFGKSRAKRVSVDSPKVTFKDVAGAEEAVEELHEIKEFLENPKKFQQLGARIPK
                     GVLLYGPPGTGKTLLARAVAGEAGVPFFSISGSDFVEMFVGVGASRVRDLFDQAKQNA
                     PCIIFVDEIDAVGRHRGAGMGGGHDEREQTLNQLLVEMDGFEMKDNIILIAATNRPDI
                     LDPALLRPGRFDRQIVVDRPDRPGRKRILEVHSRGKPLSKDIDLDALAAQTPGF"
BASE COUNT         1285 a         3090 c         2999 g         1295 t
ORIGIN      
        1 cggcatgccc atcctcaacg cccacgacgc cggcgccgtg atcgtctgca acctcgacga
       61 gaagcccggc tactccggcg tgcccaaccc gctctacgac gaccccaagg cgatcctcct
      121 cttcggcgac gccaagggca cactcgggga gctgatcagc gggctggaga acgcggccta
      181 gacgtatcgt tggcctggcg cggcggagca ggacgcgccg tccaatggcc aaggccatgt
      241 cgccgtgcca gaatgggacc gcgtccggaa atccagtccg ggcgcggttc cttccgttcg
      301 gcgcgcgcac ctcgccacgg cgggctccgt cgtccgcggt ggccgcggcg cagcggaagg
      361 agagccggtg acgccgcttt cacggcacaa aggagcatga atgggcgacc tactccgcat
      421 cgacctctcc acgcgtacgt tctcagagga gaccgtcccg ccgcagctca tcaaggagct
      481 catcggcgcc aagggcatcg gcacgcacta tctgtgtgag gaggtcggcc ccgaggtcga
      541 tccgctgagc ccggacaaca agctcatctt cgtcgtcggt cccatgggcg gcaccacgat
      601 gctcggcagc aaccgctacg ccgtctactt cgcctcgccg ctcaccggcg gctacggcga
      661 gtgctactcg ggcggcaacc tcaccccgca gttcgccaag accggctaca aggtggtcat
      721 cgtcgagggc aaagcagatg cgccgatcta cctcgaggtc agcgaagagg gcgccgccat
      781 ccacgacgcc tccgacctgt ggggcatgga cgccttcaag gccgaggacg agatcctggc
      841 gcgcacgccg cacaagaagg cccaggcgtg cgtcatcggc caggccggcg agaggctcgt
      901 gcgcttcgcc tgcgtcaaca acaactactg gcaccaactc ggccgcggcg gccccggcgc
      961 cgtgttcggc tccaagaacc tcaagggcat cgcctggcac ggcgagaaga aggtcgaggt
     1021 cgcgcggccg gatgacttca aggccgtggt ccgcgacctc atcgaacgca ccaaggacga
     1081 ccccggagcg gcggcgtacc agcgtggggg caccctcaac atggtgcgca tcatgaacgg
     1141 cgtcaacgcg ttccccacgc gttactggcg caagggcacg ctggagaact tcgagcgcat
     1201 caccgtcgag accatgctcg ccgagcacgg gaccgtcaac cagacgtgcc cgccctgcgt
     1261 gttcaagtgc gtcaagcgca acaccgtgtt caagggtcgc cacgaggggc ttgtggtgga
     1321 gggtcccgag tacgagaccg ccttcgtctt cggcggcctc tgcgagatcg acgacttcgc
     1381 cgagatcatg tggctcaacg acatctgcga ccggctcggc gtcgacacca tggacgccgg
     1441 caacctctgc gcgctggcca tcgagtgtgc cgagcgcggc ctcatcgacg agaagctcac
     1501 ctggaacgat cccgtcggcg tcggcgcctt cctggagaag atctgcctgc gcgagggcat
     1561 cggcgacctc ttcgccgagg gcgtcgtcgc ggtcgccggg gagtacggac tcgaggacgt
     1621 ggccgtgcac gtcaagggca tggcgcctcc cggctacgag ccgcgcaaga tgaagggcat
     1681 gggcctcggc ttcgccacca cgccgcgcgg cgcctgccac ctgcgcgcca ccatgtacaa
     1741 gcccgagctc atgggggtca cgacctacgg cgaggtcgac gacaaggcgc cggtctacgt
     1801 cgactgggaa gaccgcctca cgatcatgga cacactggtc tactgccgct tctaccgcga
     1861 catggtccag tggccgtaca tcaccgcggt cgtcaatgcc gtgatcggca ccgactacac
     1921 cgaagcggac ctgcacggca tcgccaaccg catcatcacc atgacccacg acttcaacgc
     1981 cgcgcgcggc atcgggcgtg agtcggagaa gctgcccgac tgggtcaccg agaagcccat
     2041 ggaggacgag gagggactca ccttcccgca ggaagagatg gcctccatgc tccaggacta
     2101 ctacacgctg cgaggctggg gggagttgcc gccgctcgcc ggctgaggcg aagcggcggg
     2161 gacgcggacg ggcccgggac cctcgcgcga gggtcccggg cccgtccgtg ctcggggcgt
     2221 gagcggctgc gccgcccgcc ccggtctccg gcttcttcta gatgcccagg tcgctccact
     2281 ccttctcgcc ggagagccag ccggcgatga ggtagtacgg gcgcatggcg tcgttgaagg
     2341 tctgcgtgac gacctgcaga cgcgcggcgg agcgctgcat ccgggcgagg ttgtcgccga
     2401 gttgtcccgc gtcggcggag aggcgctctg ccgcgacggt gatctcgtcg gtcctgcgtg
     2461 cgaggccgtc ggcgaggggc gcgacgcgcc gggacacggc catgccgtgc ttcgccaggc
     2521 gccagccgat gaggccgaga cggacgagca tggcgacgac gagcgccagg ctcgcaagcg
     2581 cgatgatcag gaggacatac caaggcatga cggcagtgta ccgcttcgcg ccgggcggcg
     2641 acagtgctct cccgatcgcc gcggtcaggg caggctgagc gagtacctgc agatgagcga
     2701 gcagccgcgg cccgccagcc ggctcacctt gaccacgtcg ccggtgtgcg gcgcctcgat
     2761 gaaaagaccg ttgccgatgt agatgcctac gtggtggtag aaggccggcg tgccgaagaa
     2821 gacgaggtcg gcaggctgag cctgcgccag cgacacaggc ttgcccatgt gcgcctgcat
     2881 ggtggccccg tgcgggaaca ccacgccgaa cttggcgtac acgtacagca cgaggcccga
     2941 gcagtcgaag ccgccggagg gtgtcgctcc ggcccacacg tacgggatgc cgaggtactt
     3001 catcgtctcc ctgaccacgg cgatctgcgc cggcgcgccg tggatggtgg ccaggtcgac
     3061 gccggcggcc ctggccaggc gcgcggacgc cgccgcgtcg agcttggcct gctgtgccag
     3121 cagcttcttg acgcgcgcgt cgaggtccgc gaggagcgtc tcgcgcgccg ccagctggtc
     3181 ctcgatcgcg gcctgctgct cacgcagggc catctccttg tcgagcgcct ccgcgcggtc
     3241 ggcgtcggtc tgcgtcgcga gcgcctggat ccgggtctcc atggccgcga tgcgggtgac
     3301 ggtctcctgg tccgcctcgt cgatggagcg gaagtagccg agctgcgtgt ccgcctcgcc
     3361 gaggtcgctc aggctgaaga gcacgtcgag cacgccgtag ccgtcactct tgtacatggc
     3421 cgcgaggcgc gccccgcgca gcgcctgggc cgtctcgagc tcgacctgcg cgcgctcgag
     3481 gtcgcgccgc gtctccagga gacggacgtt gagcacgtcg agctcggcgc gcgcgaggtt
     3541 gtacttctcc gtcaggatcc cggcgcgatg gtccaggcgc gacacctcac gccgcacgcc
     3601 gcgcgcctgc gcctcgagat cctggactga ggcgggcgtg gccggggcag cgggtgggtg
     3661 cacggcggcg ccgagcagga tcagcgccag cacaccggcg aagcacgccg cggcaagggc
     3721 cgccgcgctc cgctggaccc acgtgcctgt cgcctcgaag ccggtcatct tcgctgcagt
     3781 gtatcggaac atcccggctc tcctttacgc cgcgcgagag gggcgtccct gccgggcgct
     3841 ttacaatcgc cgttcgcgcc gtgtacaatc tgtcgtccaa cgtcgcgggg tggagcagtc
     3901 cggtagctcg tcgggctcat aacccgaagg tcgcaggttc gaatcctgcc cccgctacca
     3961 tcaccttcct gggcgcccgt tcgggcgcct ttcttgttgg cggcgtgaga acggcgctcg
     4021 tcgggtaacc atcgcctcga tcgtagaaag tcgaaaagct gacgggtggg gtgggcgtat
     4081 gaacactggc agagccgcgg tcgccgagtt cttcgggacg ttctggctcg tcttcggcgg
     4141 cacgggcgcg gccgtcctgg ccgccgcggt cggggacaac ggtatcgggt ggctcggcgt
     4201 gtccctcgcc ttcggcctca cggtcgtgac catggcctac gcggtgggcc acatctcggg
     4261 cgggcacttc aaccctgccg tcaccgtagg gctgtgggcc ggcggtcgct tcccgggcgg
     4321 caaggtggcc gtctacatcg ccgcgcaggt cgtgggcgcc gtcgccgcgt ccgccgtgtt
     4381 gtacgtgatc gcctccggga gggccggttt cacgctcgcc gacggcttcg ccgcgaacgg
     4441 ctacggcgac cactctccgg ccggctactc gctcgccgcg gcgatcgtga tcgaactgct
     4501 cctcacgttc gtcttcctgc tcgtcatcca cggggtgacg gaccggcgcg ctcccgcggg
     4561 cctcgcgccg ctcgccatcg gcctcacgct ggccctgatc cacctgatca gcatcccggt
     4621 gaccaacacg tcggtgaacc ccgcgcgcag cctggcgccg gcgctcttcg tgggcgactg
     4681 ggcgctggcc cagctctggg tgttcatcct cttcccgacg atcggcggca tcatcggcgg
     4741 gctggcgtac cgctacctgc tggatgcgcc ggcgagcgag cgagacaagg tcggagggcc
     4801 gggcgaggca acggagtgac ctccgcggcg gcggccgtct cgtcgaccgg aagggccccc
     4861 gccgtgcggc acggcggggg cccttttctt ctgccgccag acgaccggtc ggcgcacggg
     4921 gatcggcgcg accatgcggt ctgttgcgca ggaaggcgac gaaggtcgga ctcctcatct
     4981 gcagtcaggc ggagacctcc gggaagtcat gttcctcgtc gccgcctccg gtccggtgcc
     5041 cgtcccccct ctgctatgct cgcggcgcgc cccgtgcctg cgcgggcgcc cggaggaggc
     5101 tgtgatgtcc gtcctgctcg tcgtcaacgc gccggcccgc ggcgctgaag gatccctcta
     5161 tgcgttccga catgcggacg ccaccaccgg gtcggcggcg ggcgacgacc gcgtggtccc
     5221 ggcctgaggg cggccgtggc gcgactgccg gcagcgtgcc ttcggctcca gcggagcgtc
     5281 gcggagtcga tcgccgcgac tcgagcgctg cgcccgggtg agcacgcgct gctcctgctc
     5341 tccggcggcg ccgactccat ggcgctcctc tcgctcgtcc gggcagcgga ccgccgtctt
     5401 gggctcgggc tgcgtctcgc tacgctgcat gtggactacg gactgcgcgg cgccgactcc
     5461 gaccgcgacc gcctgatcgt cgagcgcgcc tgcgccgggg ccgggctgcc gttccacgtg
     5521 gagcggctcc acggcagcct caacgggcgg gacttccagg cgcgcgcccg cgcgcagcgc
     5581 tacggccggg cgcgccagct caccgcggag cacggctacg acgtgatcgt cacggcgcac
     5641 aaccgcgacg accaggcaga gaccgtggtc taccggctgg ccaagtacgc gtcgccgcgc
     5701 gggctggcgg ggatgcggcc gcgggaccgc gacctcgcgc ggccgttgct cggcgccggg
     5761 gccgtcgagc tccgcgcgta ctgccgcgcg gccggcatcg agtacggcga ggacgcgacc
     5821 aacgccgagc cgcgttacgc ccgcaacctg ctgcgtctcg aggtgctgcc cctgctcgag
     5881 gcgctgaacc cgcggctcgc cgagacgctg gcggccggcg cggagcaggc tgcggccgag
     5941 gcgcaggtgc tcggcgaggc ggccgccgag gcccgcgccc gggtggaacg gccgcgcggc
     6001 cgcggtgacc acgcggccgt cgacctcacc ggcctggcgg cggagccggc ggcgctgagg
     6061 gcgctgctgc tgcacgaact gctgcgcgag gcgatgggcg gcgatgccct cgtggagcgg
     6121 cgcctcgtcg aggcggtcct gcgcctgacc gaacgcgcgg acgacgccgg ccgcgtgagc
     6181 ctcggccgcg ggctcgaagc ggtgcgcggc ggcggcgtgc tgcgcatccg gcccgtcgcc
     6241 gcgggtcacg tctgcgctcc gctcgtcctc gagggtgtgg gtctggccgc cgccggggag
     6301 gccggcgtca ccgcccgctt ctgcggcggc cgctggcgcg tccgcctgct tcccggcgcg
     6361 gcgctcgatc gtgcggcggc gatcgccggc gaagggttcg ccggcctggc cgggccaccg
     6421 cggcgcgtga cactgcggca tccgcgccgg ggggagcggt tcgcgccgct cgggctcggc
     6481 ggcgagacca ccgtcgcccg ccacctcgcc gctgcccgcg tcccggccga gcggcgcccc
     6541 ctcgcggtgg tgctcgacgt cgacgggcgc gccgcctggg tgggcggcgc cgcgcccgcg
     6601 agggttgcgc agcccttccg ggtggcccag agtagtgtcc tgacactgca cgtcgtccag
     6661 gagggaacgt gaacgtgccg atcgccgagg tactcgtcga ccgcgaggcg ctggccacgc
     6721 gcgtggacga gctggccgtc cagatcaccg ccgactacgc gggcaaggag ctcctcctcg
     6781 tgggggtgct caagggcgcc gtgttcttca tggccgacct cgcgcggcgc atcgacctgc
     6841 ccgtcgccct cgacttcatg gctgtctcca gctacggctc gtcgaccgat tcctccggcg
     6901 tcgtccgcat cctcaaggac ctcgacctcg agatcgccga ccggcacgtg ctcatcgtcg
     6961 aggacatcat cgattcgggc ctgacgctca gctacctgct caagaacctg cgcagtcgca
     7021 agcccgcgtc gctcgagatc tgcgccctcc tcaccaagcc gtcccggcgg cgcaccgaga
     7081 tcccgtgccg ctacgtcggc ttcgagatcc ccgacaagtt cgtggtcggc tacggtctcg
     7141 acctggcgga ccgcttccgc acgctcgact acatcggcgt cctgaccccc gaggcggccg
     7201 ccgggatcgt cgaccaggcg ggttctttgc tgtagtcgga cgggtatgtt acggtcaccg
     7261 gacggtcgat ttgcaggcag atcgaggtac cctgtcggcc ccggagtccg cccccgtcca
     7321 gaagggtcac cgtgagcaga ttcatccgta gcgcgctgtt tcccatcctc atcgtcatca
     7381 tcgtggcgat gttcatcgag tgggtcatct ccggcaaccg ggccaacgag gcgccgaagg
     7441 ccatctactc ggcgccgttc gtcaccacgc agaccgtgct gacgagcgac ctgcagaacg
     7501 gcctcgtcaa gaacgtgatc ctcgacccga cctccggcgc cgccaagatc acccagaccg
     7561 gcgggctgca gtactacgtc gaggacatca cggatgcgca ggcgcttgcg ctcgaggagc
     7621 agatcggctc cgaccagccc gaggtcgtgg tcatcaaggg cccggtcgca ccaccggccg
     7681 ccaaagacgt ccagcccagc ttcaccaacg acgtgataaa cggcagggtc gacgccgtcg
     7741 tgatgaacgt caaggaccag acgatggagg tcaccctcca gccggtcgac ggcaaggcgc
     7801 agcagtacac cgtcgcctac accgatccgg cggcgacgac cgcgttcctc gaccagaaca
     7861 acgtgcccta cgacgccacg agcccgaagt cggcatggtg gaccagcgcc ctgttcacca
     7921 tcctgccgat cctgctgatc ctcgggttct ggttcttcat gatgaaccag atgcaggggg
     7981 gcggctccaa ggtcatgagc ttcggcaaga gccgcgccaa gcgcgtcagc gtcgactcgc
     8041 ccaaggtgac gttcaaggac gtcgccggcg ccgaagaggc ggtcgaggaa ctccacgaga
     8101 tcaaggagtt cctggagaac cccaagaagt tccagcagct cggcgcccgc atccccaagg
     8161 gcgtgctgct gtacggcccg cccggcaccg gcaagacgct gctcgcgcgt gccgtcgccg
     8221 gcgaggcggg cgtcccgttc ttcagcatca gcggctccga cttcgtcgag atgttcgtcg
     8281 gcgtcggcgc ctcgcgcgtg cgcgacctgt tcgaccaggc caagcagaac gcgccctgca
     8341 tcatcttcgt ggacgagatc gacgccgtgg gccgtcaccg cggcgcaggc atgggaggcg
     8401 gtcacgacga gcgcgagcag accctcaacc agctgctcgt cgagatggac ggcttcgaga
     8461 tgaaggacaa catcatcctc atcgcggcca cgaaccgccc cgatatcctc gatcccgcgc
     8521 ttctgcggcc gggccgcttc gatcgtcaga tcgtcgtcga ccggccggat cgtccgggtc
     8581 gcaagcgcat cctcgaggtg cactcgcggg gtaagccgct cagtaaggac atcgacctcg
     8641 acgctctcgc ggcgcagacg ccgggcttc
//
LOCUS       DASUMX010000136         5354 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_42624, whole genome shotgun sequence.
ACCESSION   DASUMX010000136 DASUMX010000000
VERSION     DASUMX010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 5354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5354
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42624"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="VFZ86_06530"
     CDS             <1..312
                     /locus_tag="VFZ86_06530"
                     /inference="COORDINATES: protein motif:HMM:NF025888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HEX5641983.1"
                     /translation="IIRGVNVYPSQIENVLARTEGVQPHYLIVVDRKRNLDDIEVRVE
                     VDEALFSDVMGDMVRFQRKVAERIQAELGLRARVTLVEPGANERTADPTRRVLDLRQR
                     D"
     gene            complement(320..396)
                     /locus_tag="VFZ86_06535"
     tRNA            complement(320..396)
                     /locus_tag="VFZ86_06535"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(360..362),aa:Asp,seq:gtc)
     gene            complement(559..1782)
                     /locus_tag="VFZ86_06540"
     CDS             complement(559..1782)
                     /locus_tag="VFZ86_06540"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX5641984.1"
                     /translation="MNHHIEHLADELEERGHETWIIAPVGAVTPARRSVDSRRQAMAE
                     RFIPMGSAVPLPSNGSLAYVNVSPQIFARLDRAIRYSRFDVLHVHEPCTPMVSGMAVV
                     MATSPVVGTFHAALDDSPYYDSLKPLVSTVMRHLDVRVAVSEAARAFPQSRYPGEYRI
                     IPNGIPVEKYAPAVGAPKVRGRILFVGRAERRKGLGVLLQAFTLLRERAPHATLVVAG
                     ATRRQLLETDRNGFRLPVDLAGVEALGWVADEEKIAHLAAAEMVCAPSLAAESFGIVL
                     AEAMAAGVPVVASDLPGYRAVLRDGQAGRLTPPGDPVALADALYDLLQHEEERRRLAA
                     AGSAAAAELSWSRITGSIVEAYEDALAAPRVRGMHGTPGRPWFGRAILDHTTWLVRNG
                     YLSGRAKDRDGDRRG"
     gene            complement(1844..2473)
                     /locus_tag="VFZ86_06545"
     CDS             complement(1844..2473)
                     /locus_tag="VFZ86_06545"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: protein motif:HMM:NF014690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HEX5641985.1"
                     /translation="MSRRGERRHGALHTLAGLAGGFAAGAIPTGELVTRALTGRRLRD
                     LGDGKAGSSNVARSVGWKAGAAVLALDALKAYVPATAAGLRGAGDNEVAAIGVSAMCG
                     HIAVVKGRGAASALGAAFAMDPAMMAVGCVPLVGGSLLHRHAEAVTVTALSLPLISLA
                     LHRSPRRALGPLLLITVLFAARLRGSPGAPLPDSPKVLWNRFLLDRDER"
     gene            complement(2470..3663)
                     /locus_tag="VFZ86_06550"
     CDS             complement(2470..3663)
                     /locus_tag="VFZ86_06550"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX5641986.1"
                     /translation="MPTTVEGHLAASTSAERAPRTLFVTNDFPPRIGGAQSYYWGVIR
                     TLDPSEVAILAPAHIDAAAFDATHPYTVVRASTSVLWPTNAMLHTVEQMAEREGAELI
                     QLGHPLPAGLLGPRLRQRRGLPYVVFLGGAEVTLPGVVPGVNNMLRHVLGNASMLLTV
                     SEYTAEAARKQTHGKVPAEVLRPPLHVDEFEPADGDEVACLRERLGIDGQIVVCVGRL
                     VPRKGQDKLIDALALLRSEFPRLHLVLIGEGRLATGLYDRAQKRGVGERVHLTGALTD
                     QAMKEWLRAADLFASPCRTRWAGLEVEGFGIVFAEAALAGLPVIAGDSGGAPEAVIQG
                     DTGLVVDGHSVQQVAAALASMLRRTPEQRRVMGAKGRELALARHTPAVVGERYRELLR
                     RAAAR"
     gene            complement(3721..4485)
                     /locus_tag="VFZ86_06555"
     CDS             complement(3721..4485)
                     /locus_tag="VFZ86_06555"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX5641987.1"
                     /translation="MGVPSERELARERQSHVRHELRAPIAVIYPLLSLLLDGGAGELS
                     AQQRDYLEMLERSVVRIEELVTGAAESGWADCSAAPAEPSEVVLEDVAREVVTVRRMT
                     GQGGPSVEVDAAAPSPCAWADRDDVRQIVTDLVRNAVTYAPDDGRVVIRTAAGEGAGT
                     AVIEVGDNGRGMSPEELARAFEFGFRGELATQLRVPGLGAGLWICRELAARNGGSVTL
                     ASEPGAGLTATLALPAFGSAAAGAAPVLTRPEGHGG"
     gene            complement(4491..4994)
                     /locus_tag="VFZ86_06560"
     CDS             complement(4491..4994)
                     /locus_tag="VFZ86_06560"
                     /inference="COORDINATES: protein motif:HMM:NF015669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HEX5641988.1"
                     /translation="MREIRDTQAMAASSPLHDVRLRAAILTAAALLAIALIAAVVAGC
                     GGESSPSLEGTSWKLTGWSAGSQDPNDFTITAEFKDGRIGGTSAVNSYGGPYTTGDDG
                     SFSVGELASTMMAGPEADMQAETDYMTLLAAAKEFRVDGETLTLLDGDGGESLIFTAT
                     SGASPEE"
     gene            complement(5079..5153)
                     /locus_tag="VFZ86_06565"
     tRNA            complement(5079..5153)
                     /locus_tag="VFZ86_06565"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5119..5121),aa:Val,seq:tac)
BASE COUNT          791 a         1935 c         1908 g          720 t
ORIGIN      
        1 atcatccgcg gcgtcaacgt gtacccaagc cagatcgaga acgtgctggc acgcaccgag
       61 ggcgtacagc cgcactacct catcgtcgtc gaccgcaaga ggaacctcga cgacatcgag
      121 gtgcgcgtcg aagtggatga ggcgctcttc tcggatgtca tgggcgacat ggtgcgcttc
      181 cagcgcaagg tggccgagcg gatccaggcg gagctcggcc tgcgtgcccg ggtgaccctc
      241 gtggagccgg gcgccaacga gaggacggcc gacccgacac gccgcgtcct ggacctgcgg
      301 cagagagact gagaggaagt ggcgggagcg acggggctcg aacccgcggc ctccggcgtg
      361 acaggccggc gctctaacca gctgagctac gcccccgcga aaggacgctg acggccggtc
      421 acggagcgac cggccgcgtt gcgaatgcgg agtctagcat acgcctcgcg gcgcggcaaa
      481 acagcgcggc gctgtggttg agaggggcgg acaccgtcgg atgagccggc tcgtcggcgg
      541 acgaccgggc cgtaaccgtc aaccccggcg gtcgccgtcg cggtccttgg cgcggccgga
      601 caagtagccg ttccgcacca gccaggtggt gtgatccagg atggcgcggc cgaaccacgg
      661 cctgccgggc gtaccgtgca tgccgcgcac ccgcggcgcc gccagggcat cctcgtaggc
      721 ctcgacgatg ctgccggtga tgcggctcca ggagagctcg gcggcggcgg cgctgccggc
      781 ggcagccagg cgccgccgct cctcctcgtg ttgcagcagg tcgtacagcg cgtcggcgag
      841 cgcgacgggg tcgccgggcg gcgtgagccg cccggcctgg ccgtcgcgca acaccgcgcg
      901 gtaccccggg aggtccgatg cgaccacggg cacgccggcg gccatcgcct cggccagcac
      961 gatgccgaag ctctcggccg ccagcgacgg cgcacagacc atctccgccg ccgccaggtg
     1021 ggcgatcttc tcctcgtcgg cgacccagcc gagggcctca acgccggcca ggtcgaccgg
     1081 cagccggaat ccgttgcggt cggtctcgag cagctgccgg cgagtggcgc cggcgacgac
     1141 cagcgtcgcg tgcggagcgc gctcgcgcaa gagcgtgaac gcctgcagga gcacgccgag
     1201 ccccttgcgg cgctcggcgc gccccacgaa caggatccgg ccgcgcacct tgggcgcgcc
     1261 cactgccgga gcgtacttct ccacagggat cccgttgggg atgatccggt attcgcccgg
     1321 atagcggctc tgcgggaacg cgcgcgcagc ctcgctcacg gccacgcgca cgtcgagatg
     1381 gcgcatcacc gtgctcacga ggggcttgag gctgtcgtag tacggcgagt cgtcgagcgc
     1441 cgcgtggaag gtgccgacaa cgggtgatgt cgccatgacc accgccatgc ccgagaccat
     1501 gggagtgcag ggctcgtgca cgtgcagcac gtcgaagcgg ctgtaacgga tggcgcggtc
     1561 caggcgcgcg aagatctgtg gcgagacgtt cacgtaggcg agcgagccgt tgctcggcag
     1621 cggtacagcc gagcccatgg ggatgaagcg ttcggccatc gcctggcggc ggctgtcgac
     1681 cgagcgccgc gccggcgtca cggcgcccac cggcgcgatg atccaggtct cgtgaccgcg
     1741 ctcctcgagc tcgtcggcga ggtgctcgat atggtggttc accccgcccg ggacggtcca
     1801 ggagtacggg gatacgaggc ctacgcgcac ccggagcctc cgctcagcgt tcgtccctgt
     1861 cgagcaggaa ccggttccaa aggaccttgg gcgaatccgg caggggggcg ccgggcgacc
     1921 cacgcaggcg cgccgcgaac agtacggtga tcagcaggag cggcccgagc gccctccgcg
     1981 ggctgcggtg cagcgccagg ctgatgagcg gcagcgagag cgccgtcacc gtcaccgcct
     2041 cggcgtgacg gtgcaggagg ctgccgccga ccaggggcac gcagccgacg gccatcatcg
     2101 ccgggtccat ggcgaacgcg gcgccgagag ccgaggcggc gccgcgcccc ttgacgacgg
     2161 cgatgtgccc gcacatcgcc gagacaccga tggcggcaac ctcattgtcg ccggcgccgc
     2221 gcagcccggc cgcggtcgcc ggcacgtacg ccttgagcgc gtccagggca agcacggcgg
     2281 cgccggcctt ccagccgacg ctgcgggcga cgttggacga gcccgccttg ccgtcgccca
     2341 gatccctgag ccgccggccg gtgagggcgc gggtgacgag ctccccggtc gggatggcgc
     2401 ccgcggcgaa tcccccggcg aggccggcca gcgtgtgcag cgcgccgtgg cgccgctctc
     2461 cgcggcggct catctggcag ccgccctgcg cagcagttcg cggtaacgct cgccgacaac
     2521 ggcgggtgtg tgccgcgcca gcgcgagctc gcgtcccttg gcgcccatga cccggcgctg
     2581 ctccggcgtc cggcgcagca tcgacgccag cgcggcggcg acctgctgga ccgagtggcc
     2641 gtcgacgacc aggccggtat cgccctggat cacggcctcg ggcgcgccgc cggagtcgcc
     2701 ggcgatcacc ggcaggccgg cgagcgcggc ctcggcgaag acgatgccga atccctcgac
     2761 ctcgaggccg gcccagcggg tgcggcacgg cgacgcgaat agatcggcgg cgcgcagcca
     2821 ctccttcatc gcctggtcgg tgagtgctcc ggtgagatgg acacgctcgc ccacgccgcg
     2881 cttctgcgcg cggtcgtaca ggccggtcgc caacctgccc tcgccgatga gcacgaggtg
     2941 cagacggggg aactcgctac ggaggagcgc gagcgcgtcg atgagcttgt cctgaccctt
     3001 gcgggggacg agacgtccga cgcagacgac gatctgcccg tcgattccga gacgttcgcg
     3061 taagcacgcg acctcgtcgc cgtctgccgg ctcgaactca tccacgtgca gcggtggccg
     3121 gaggacctcc gccggcacct tgccgtgggt ctgcttgcgc gcggcctcgg cggtgtactc
     3181 actcacggtg agcagcatcg acgcgttgcc gagcacgtgc cgcagcatgt tgttgacgcc
     3241 cggcacgacg ccgggcagcg tgacctcggc gccaccgagg aacacgacgt agggcaggcc
     3301 gcgccgctgg cgcagccgcg gcccgagcag gccagcgggc agcggatggc cgagctggat
     3361 gagctccgct ccctcgcgct cggccatctg ctcgaccgtg tgcaacatcg cgttcgtcgg
     3421 ccagaggacc gacgtcgagg cccggaccac ggtgtaggga tgggtcgcgt cgaacgcggc
     3481 ggcgtcgatg tgcgccggcg cgaggatcgc gacctccgag gggtccaggg tgcggatcac
     3541 accccagtag tacgactggg cgccgccgat gcgcggtggg aagtcgttgg tgacgaagag
     3601 cgtgcgcggc gctcgctcgg ctgacgtgct ggctgccagg tgtccctcca ctgtcgtcgg
     3661 cacgggcatc cgccggggac gcgtgcgcac cactctacca tgaccgcagt ccgcgcccgg
     3721 tcagccgccg tggccctctg ggcgtgtcag gacgggtgcc gcgccggcgg cggcgctgcc
     3781 gaacgccggc agcgccagcg tcgccgtcag gccggccccc ggctcactcg caagcgtcac
     3841 gctgccaccg ttgcgcgccg ccagctcgcg gcagatccag aggccggcgc caaggccggg
     3901 tacgcgcaac tgcgtcgcga gctcgccgcg gaagccgaac tcgaaggccc gggccagctc
     3961 ctccggagac atgccgcgcc cgttgtcgcc cacctcgatc acagccgtcc ccgcaccttc
     4021 gccggcggcc gtgcggatca cgacgcggcc gtcgtccggt gcgtacgtga cggcattccg
     4081 caccaggtcc gtgacgatct gccgtacgtc gtcccggtcg gcccacgcgc acggcgaggg
     4141 cgcagccgcg tcgacctcga ccgacggccc gccttgcccg gtcatgcgac gcacggtgac
     4201 gacttcccga gcgacatctt cgagcaccac ctcagagggc tccgccggcg ccgccgagca
     4261 gtccgcccag ccgctctcgg cggctcccgt gacgagctcc tcgatgcgca cgacgctgcg
     4321 ctccagcatt tcgaggtagt cgcgctgctg ggcgctgagc tcgccggctc ccccgtcgag
     4381 cagcagagag agcaacgggt agatcaccgc gatgggggcg cgcagctcgt gtcgcacgtg
     4441 cgactgccgt tcgcgcgcga gctcccgctc cgagggtacg cccatgccgc ctattcctcc
     4501 ggcgaggcgc cgctcgtcgc cgtgaagatc agcgattcgc cgccgtcacc gtcgaggagc
     4561 gtgagggtct ctccgtcgac ccggaactcc ttcgccgctg ccagcagagt catgtagtcc
     4621 gtctccgcct gcatgtccgc ctccgggccg gccatcatcg tgctggcaag ctcgccgacg
     4681 gagaacgacc cgtcgtcacc cgttgtgtac gggccgccgt agctgttgac cgccgacgtg
     4741 ccgccgatgc ggccgtcctt gaactcggcg gtgatggtga agtcgttggg atcctgactg
     4801 ccggccgacc agcccgtgag cttccaggaa gtgccctcca gcgacgggct cgactcgcca
     4861 ccgcaaccgg cgacgaccgc ggcgatcagc gcgatcgcga gcagcgccgc agccgtcagg
     4921 atcgctgcac gcaggcggac gtcgtgaagg ggcgaactgg ccgccatagc ctgcgtgtca
     4981 cggatctctc tcatcacctg actcctctcg tcgccgctgt ccccagaatg acgacgcggc
     5041 ggcggccgag gttcgcgaac tgcagggcgt actgcgagtg gtgggcgatg ctggatttga
     5101 accagcgact ccctgcttgt aaggcaggtg ctctacccct gagctaatcg cccgcgcgcg
     5161 gctaagtata cgacgaccgc cacggctcgc cttgtggcga ccactcacgc tccacgagcc
     5221 gctgccgcaa gaagaagggg cgggaggccc ttccggcctc ccgccccgcc ttcactacga
     5281 tgtccgctcg cctaaagcgg cgtcacctca cgccaagtgt tctgcacccg gcgagccatg
     5341 gacgggaacc gctg
//
LOCUS       DASUMX010000137        31529 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1623417, whole genome shotgun sequence.
ACCESSION   DASUMX010000137 DASUMX010000000
VERSION     DASUMX010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 31529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31529
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1623417"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1060)
                     /locus_tag="VFZ86_06570"
     CDS             complement(<1..1060)
                     /locus_tag="VFZ86_06570"
                     /inference="COORDINATES: protein motif:HMM:NF013216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEX5641989.1"
                     /translation="MSGSGPGETTGRSGVSPARAAAFAALRRLRAGARLDESAAALPE
                     LSSLGPADRGLANELVNGTVKRRGSIDAVLGAYTKAPLKTADPDVRDALRLAAFQLLF
                     LDRVPAYAAVDDAVTLATRKSRRAGGFVNAVLRRVATDGRAKLEELGEGDAVRAWSVG
                     LSYPAWLVKLLREDLGDAAARRLLESANAAPERCLRANRLRGGVPAAAEALAAEGFAT
                     RGIDGLPDGLLYEGPPLERSRAFREGLVTPQSRGSQIAGMVAAGAVTGPAAAVLDLCA
                     APGAKTSQLAALQAGAAITAVEVDEDRAGELRENLARLGADHIAVVQADALEARPEWD
                     EAFDAVLLDAPCSGFGTLA"
     gene            complement(1057..1950)
                     /locus_tag="VFZ86_06575"
     CDS             complement(1057..1950)
                     /locus_tag="VFZ86_06575"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEX5641990.1"
                     /translation="MRLAFAGTAPFAELVLHGLAGAERTPVVLVTNPDRPRGRHGTPQ
                     APPIKLTAEALHIPVFQPERLSDPEALERLLETKPDVLVACAYGQIVAQRVLDAVETI
                     VVHPSLVPRWRGAAPVERALMAGETELGVCTLRMTAGVDEGPIGDARRVHVPRDADAG
                     RAYELLAPAAVEGVAATLDGIAAGTVEWRAQEGPVTYAAKIGPRDKEIDWSRPATAIA
                     DQVRALSPHIGAVTELAGRRTRIWRAAPGHGPVPETGPDRLVVAAGEGWLDVHELQQE
                     GRGRVTAAEFLRGAGRGLLAP"
     gene            complement(1956..2537)
                     /gene="def"
                     /locus_tag="VFZ86_06580"
     CDS             complement(1956..2537)
                     /gene="def"
                     /locus_tag="VFZ86_06580"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX5641991.1"
                     /translation="MSETTQIRETADAQDLSRRQREARRDIRVIGDPVLREHAREVTE
                     FDRGLRKLAKRMIRTMKDAPGVGLAAPQVGVLQRLLVYDVDDDPRVLANPVLDEFSEE
                     TEEADEGCLSVPGVTMPVTRPVSLRVRGLDEYGEPVDFRAEGFEARVIQHENDHLDGV
                     LIVDRTSRSARAAALRAMRANEAGGIEPAGGGL"
     gene            complement(2564..4984)
                     /gene="priA"
                     /locus_tag="VFZ86_06585"
     CDS             complement(2564..4984)
                     /gene="priA"
                     /locus_tag="VFZ86_06585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEX5641992.1"
                     /translation="MSPYAAVFPLVRTRAFSEPFDYEIPPELAGGALPGALVAAPLGA
                     QTVIGVILGVGDHTAHEGRVLPLRDVLRVPPIPEELLVVARRIERHYLCSFAAALALV
                     CPPTGALKIERRYELTDGGHAAAEAGEAKLLELAGLHLRSGPLAADAERYRRKGWLRV
                     AYRVRVVGVTPPGRMLRRGTETPSRLGARQRAALEIVEEAGSLDERELRAASDLSAAG
                     LRRLLAAGALLAEDPPASFAAVAGDETGGGRPGSPAEADAGVSVRAAAQAARLRACVT
                     LSDVPDLLDEQRRALHSLLCETRPGDEVLLHGVTGSGKTEVYLQAAQATLETGRSVLL
                     LVPEIGLTGQTVARVRRRFAGAQVAVLHSGLSAGERLLAYSAVARGEVRIVVGARSAV
                     FAPLVDLGLIVVDEEHDTSYKQESEPAYDARTIARWRAEGTGAVVVLGSATPSVESFA
                     RVAQHADLHRRVDGSRPPELEVVDMREHHGVFSAPLAEALSGVVDAGEKAILFLNRRG
                     FASYLVCDHCGHAWECPRCDVTLTLFGSHTLRCRTCGHSEPAPSACPECGRSDLVRHG
                     FGTERLEREVRLLLPGVDLLRLDSDVAGSYARLQGVLSRFAEPGPKVLVGTQMIAKGH
                     HFPDVTLVGVVNADLTLHFPDFRAEERTFALLVQVGGRSGRGDRPGRVIVQTLDPQAR
                     PIALAAAGKEEEFYAEELERRRELGYPPAGFLVGLDISGTSRDKVEVAGRFTAERLGA
                     RLAGTAQVLGPGPLWRERGRHACRVVIKTTETGKTLDALRAWLATNRDRYAARGVRVV
                     PDVEPQWL"
     gene            complement(5013..6227)
                     /gene="metK"
                     /locus_tag="VFZ86_06590"
     CDS             complement(5013..6227)
                     /gene="metK"
                     /locus_tag="VFZ86_06590"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018132148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEX5641993.1"
                     /translation="MDKRNYLFTSESVTEGHPDKLADQISDSILDAILKDDPYGRVAC
                     ETLVTTGLAFVAGEITTETYVDIPKIVRQTVKEVGYTSAHYGYDYETCGVMVAIDEQS
                     PDISQGVTTALEVRTDVNDDDALDTQGAGDQGMMFGYACTETAELMPMPILLAHKLAK
                     RLADVRKAEILPYLRPDGKSQVTVRYEDGKPVEIVKIVLATQHAESADLQSIIYPDLL
                     EHVVEPIMPKHMYDVGKLEDITVINATGKFVIGGPMGDCGLTGRKIVVDTYGGAAPHG
                     GGAFSGKDPSKVDRSGAYAARYVAKNVVAAGMAEKCQVQVAYAIGVAHPVSVMVDCGG
                     TEKVELVTIERLIGEHFDLRPAAILRDLDLRRPIYRKTAAYGHFGRNDHDFTWERTDK
                     AEALRAAAGLTA"
     gene            complement(6360..7355)
                     /locus_tag="VFZ86_06595"
     CDS             complement(6360..7355)
                     /locus_tag="VFZ86_06595"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEX5641994.1"
                     /translation="MRATVIGSGSWGSAFARLLVRRGHDVEVLALTREEAARLQATHE
                     NPHFLPGVRLPAEIDFSGMEDADLSGAGLLVYAVPTQAVREVARWVAPRRAAGALQLS
                     LAKGYEIGSLKRPTQVIEEETGAGAAALSGPNHAEEVSKDMPSATVIAAADEAQTLAL
                     QAAITSDTFRVYTNDDVVGVEFCGAVKNPIAVAVGMSDGLGFGDNSRASLITRSIAEM
                     TRLGMIQGAHIATFTGLAGIGDLIATCTSRHSRNRLAGELLAKGASPAAVEAEVGQVV
                     EGLHTAYALHDLASRLGIDMPVTENVYQVLEGNRTPQECVADLMGRTPKRERHGS"
     gene            complement(7357..8004)
                     /gene="plsY"
                     /locus_tag="VFZ86_06600"
     CDS             complement(7357..8004)
                     /gene="plsY"
                     /locus_tag="VFZ86_06600"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEX5641995.1"
                     /translation="MIVVQVLGFVILFVLAYLAGSIPFGLVVGKLFYGVDVRRHGSGN
                     VGTTNVFRVLGKKAGVAVLVCDMLKGYIPAALAAALFDPWFAIFIAAAPVVGHMYSIF
                     LKGRGGKGIATGAAVVLALVPLVFAIVLVAWIVLLLTTRYVSVASLAAAFLVPVLTIS
                     LDEPVPYKIAGVLVAVIVWWAHRGNIQRLLAGEEHRVRLPWTSGGHATPDGQGGA"
     gene            complement(8001..9308)
                     /gene="der"
                     /locus_tag="VFZ86_06605"
     CDS             complement(8001..9308)
                     /gene="der"
                     /locus_tag="VFZ86_06605"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEX5641996.1"
                     /translation="MKPLPIVAVVGSPNAGKSTLVNRLTGTRATVVHETPGVTRDRKE
                     IEVEWTGHRLLLVDTGGYDTTEDAPFAGHIRGQVEHAIAAADVVLFVLDGRYGPLTDD
                     YAIADVLRRAKAPVIVVANKMDDPASTATAPEIYALGLGEPLLFSAVHGTGSGELLDR
                     LVEVTGAERGDGEEEAEPAETPVAIVGRPNAGKSSLFNAIAREKRTIVSEIAGTTRDA
                     IDSVVETDAGRFRFIDTAGMRKAAKVSGVEYYAYLRSVQSLDRAHVAIIVADATMRFG
                     ELDLSICTEATHRNCATVIAVNKCDIAEPDLEEIAGIAKRKLRQRPPVIAVSAETRRG
                     VRELLAKVAELGALYTAHISTRALNRALAELAADRAMPQKHGKRLKMYYIAQFGTSPP
                     RIAIEINDRTLVTRDFGFYVENRLRAQFGLEGVPLVIDFKGKR"
     gene            complement(9308..10642)
                     /locus_tag="VFZ86_06610"
     CDS             complement(9308..10642)
                     /locus_tag="VFZ86_06610"
                     /inference="COORDINATES: protein motif:HMM:NF016354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HEX5641997.1"
                     /translation="MPEAAGEACGGVVVADARDAASRGGLRAGDRIDTLNGRVPLDVL
                     DVEDAAADGTLWLTVLRAGHPLDLVVAPQRGEWHGISLDRGGLGDDPRTCRNRCRFCF
                     VDQVPPGLRPALSVKDDDYRLSFLHGNFMTLTNLTEADLERIEELRLAPLYVSLHAWD
                     DAARVRLMGRAAASSRAVLERLAAAGLELHLQVVLCPGWNDGDVLAETVRRAGALDAA
                     ADLGVVPVSLAAEGDLRRVTAGEARALVAAVRGWQRDFLERRGDAFVHAADEFYLLAG
                     EEPPAGGAPDQYENGVGIAAALRVEAEALAAAWTGGGDGGDGEGPRRRPRRDVSGVRL
                     LTGTLAAPVVARAAAALTPAARVPVRAFVVENHLFGAHVTVTGLLGGAEVLEALRRDP
                     LAGGEWLLAPRSWLPEDLGRTLDDLGEDDLSAACGGRLSVGDSLGGAFARLS"
     gene            complement(10635..11582)
                     /gene="ispH"
                     /locus_tag="VFZ86_06615"
     CDS             complement(10635..11582)
                     /gene="ispH"
                     /locus_tag="VFZ86_06615"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006236031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEX5641998.1"
                     /translation="MRDSDDATVAGHGSASYDEEPERSAELRHVRAGAVEIVVARYAG
                     YCYGVERALRLTGEALEGAPAPVATLGPIIHNPSVVGSLKDRGVDVVDEVDDAAVGTL
                     IVRTHGVPPDVIERARARRLNVVDATCPFVSVAQRKAAALREAGYAVVILGERDHPEV
                     VGLQGFAGADAVVVEDASGLPLERLRGKRVGVVVQTTQTRANLSALAAELAPVARELL
                     VFNTVCDATEKRQSAACELADEVDVVVVVGGRNSANTARLAQLCQAIEPRTHHIESAA
                     ELRPEWLEGAGRIGVAAGASTPDEEIEATVRALLDLCGA"
     gene            complement(11569..12213)
                     /locus_tag="VFZ86_06620"
     CDS             complement(11569..12213)
                     /locus_tag="VFZ86_06620"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX5641999.1"
                     /translation="MTGPWGYPLRGRGDTGLYTFLRWLAIPFFGGLYRCRVHGAEHLP
                     RTGPAMVVMTHKAFWDPVIAGMIFHRPLRFMAKKELFANPWAARFIGSLGAFPLDRGA
                     GDREALKTALTILERGEILLMFPEGHRQRDDAVHEFMPGVGMIALRSGAPVIPLAMDG
                     TQYMLRGGKPGLPALRGLVGPAVDLADITTRNSKAYREAAQRMHEAVTGLYARL"
     gene            complement(12210..12869)
                     /gene="cmk"
                     /locus_tag="VFZ86_06625"
     CDS             complement(12210..12869)
                     /gene="cmk"
                     /locus_tag="VFZ86_06625"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005927126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEX5642000.1"
                     /translation="MTVVAIDGPVGSGKSTVARRVAERLGYLYLDTGAMYRAVGLLAT
                     EAGVALDDEAAVVPIAAAAALRFDGDGRLFACERDVSGLIRTLEMGAAASVVSVLPGV
                     RRLLVERQRELASGAAIVMEGRDIGTNVFPGAEVKVFLTARPEVRAARRAAELRAKGE
                     DVDVEEVLAALVERDRRDSEREVAPLCMAGDAVEVDTSEMTLDQAVDAVAAVVREKVG
                     T"
     gene            complement(12866..13969)
                     /locus_tag="VFZ86_06630"
     CDS             complement(12866..13969)
                     /locus_tag="VFZ86_06630"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HEX5642001.1"
                     /translation="MNTVALVGVGLMGGSLGLAARERAGVAHVRGFSQTPATLELALE
                     RGAVTKACASVEEACAGAELIFVCTPVRLVVKHVRRALAAAGPTAVVSDVGSTKGPLM
                     RALSPSEQRRCIGGHPLCGSETAGVANARASLYHGATYFVTPGDHVDADAYQRLYGFL
                     GDIGARPVAVDPEEHDRVMAVVSHLPHVLANVLMSQAGQHAGARDALLSAGPSFRDLT
                     RIAGSNRRVWTDIFMDNRAALLASLAQFQEGLQEVLEALAAGDDTRLSDTIARAREQR
                     ERMLAAGSLPAEDLYRVMIPVPDRPGVFSEIMVALGDAGINVEDLAMHHMSPELGGTL
                     TVYVLGREVCRRATHVLGVLGYDVSTRREVDAE"
     gene            complement(13989..15002)
                     /gene="aroF"
                     /locus_tag="VFZ86_06635"
     CDS             complement(13989..15002)
                     /gene="aroF"
                     /locus_tag="VFZ86_06635"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF006421.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEX5642002.1"
                     /translation="MMVIMHEAATDEQVAHVCARVEQIGASAHLSAGERVKVIGVIGD
                     REAITQLPLEAMPGVDRVVAILRPYKLVSREFQQKDTVIDVKGARIGGGHFALIAGPC
                     SIETPEQVLAAARAVKEAGGHMMRGGAFKPRTSPYAFQGLGEDGLAMMAAAGAETGLP
                     IVTEVVDPRDLDVVCRYADVLQVGARNTQNFLLLAELGKAGKPVLLKRGPATTIEELL
                     MAAEYIVKEGNRDVILCERGIRTFETATRNTLDVSAVPVIKIASHLPVVVDPSHSAGR
                     LDLVLPLSLAGVAAGADGVLVETHPNPEHALCDGPQSLPTAVFGEYAGRVLDLARWTG
                     KSV"
     gene            complement(15005..16135)
                     /gene="hisC"
                     /locus_tag="VFZ86_06640"
     CDS             complement(15005..16135)
                     /gene="hisC"
                     /locus_tag="VFZ86_06640"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEX5642003.1"
                     /translation="MTVRFIPELAGITPYQPGLPIEAVQRRFGLEQVVKLASNEFPLP
                     PFPEVKAAVMGALDRLQRYPDGFSTDLRESLAAAYDRSPEQITIGCGTCELLYLLAHA
                     TLERGDEVVLAAPSFTSYRDVVAIRGAVAVTVPLADHTHDLDAMAAAVTPRTRMVFVC
                     NPNNPTGTYVSSAEVARFVERVPDDVVVVLDEAYIEFVTAEDREGSLEIQEAHDNVVV
                     LRTFSKIYGLCGLRTGYGICAPAVKQALDKVRQPFNVNLLGQLAATEALKHPDRVAER
                     RETNARLRDLLVERLAARGRATVPSQANFMLVDIDGLGVPQDEVCGALLSMGAIVRDG
                     NALGCPGWARVTVGTQEEIEFLLDKLACLEADPGEPGAGSRP"
     gene            complement(16184..17092)
                     /locus_tag="VFZ86_06645"
     CDS             complement(16184..17092)
                     /locus_tag="VFZ86_06645"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEX5642004.1"
                     /translation="MSPDQPMNLVVYLARAGVGSRRSCDELIREGAVTVNDEVVTFPR
                     QKVAAEDVVAVRGEPVAARELRYLLVNKPRGVASTRRDPHADRVIVDLVPGGRALFPV
                     GRLDVDTTGLIILTNDGVLANRLMHPRYGVPKTYVARVRGKVSRRALGTLRQGVDLED
                     GVTAPADVRLRKQSAKTALVELTIHEGRKRQVRRMFLAVGLPVEELSRRRYGPLTDKS
                     LEPGGYRELTGEEVEALRRAGEEVAGEVAKEDEGFPLMLAAEEVPAVADASLEEEDEA
                     TEPSGEPDAESEPSGEPDAPPPGDEA"
     gene            complement(17089..17664)
                     /gene="scpB"
                     /locus_tag="VFZ86_06650"
     CDS             complement(17089..17664)
                     /gene="scpB"
                     /locus_tag="VFZ86_06650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEX5642005.1"
                     /translation="MLPLARRLEALLFISPRPVAAERLADACACSEGEAATALDALRA
                     DYAAGARGIELREVAGGFTFAVADDCAELVRRFTGAEKPDDLTPALLETLAVVAYLEP
                     VTRAEIAEVRGVSSEWALSALEERGLVEQQGRADTPGAPILYGTGERFLKLFGLGSRA
                     DLPPLEEFSLGADEVDAIRSRLHANAERRAQ"
     gene            complement(17723..18514)
                     /locus_tag="VFZ86_06655"
     CDS             complement(17723..18514)
                     /locus_tag="VFZ86_06655"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEX5642006.1"
                     /translation="MSRGAATAMRADAGAVAAPPVELELDLDVFDGPFDLLVTLILRD
                     EIDIWEVRVSRIIAEYVVKLADSGEFDLDATSQFVVLVAALLEMKSRLLLEEEVEDEL
                     EDLDPDEAARQLLERLVRYSQFRNAADALRTAWEAHSARLYRTAPVPAELLRRRIPEG
                     ALPARALAEALGPLLREPPVPDTSHLADLAVSLVKELRRLRVILGDEGEFTFAAVAPR
                     GRLERAVTFFALLELHSSGEARLRQSRHFGDIVVSRIAAAAPSAG"
     gene            complement(18518..19720)
                     /gene="ftsZ"
                     /locus_tag="VFZ86_06660"
     CDS             complement(18518..19720)
                     /gene="ftsZ"
                     /locus_tag="VFZ86_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020488585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEX5642007.1"
                     /translation="MADRPSMKDGLSELFRRTDSGGAVPDPTGPDAPVKGRPYVTTIK
                     VVGIGGAGTNAVNRMVDSGVKGVEFIAVNTDVQQLEVCDADVKIHIGQEITHGLGGGA
                     NPEVGREAMEVSRDQLKAALRGADLVFVTAGEGGGTGTGGAPVVAQIARELGAVTIAI
                     VTRPFGFEGNRRQRQALEGIDALREVADTVISIPNDRLLDVVERNASVVEAFQHADDI
                     LRQGVQGVTDLITVPGLINLDFADVRTIVKDAGTALLGIGVAGGENRAQAAADMAIHS
                     PLLETTIEGARGLLLNITGGEDLTLFEVNEAAGVISEAADDDANIIFGAVVDEKLKGK
                     ISITVVATGFGDEVRPAQAPARGPEARTPAPRPEPREDRGTPFGPAPRFADKDFDIPS
                     FVNQPEDQ"
     gene            complement(19704..20462)
                     /locus_tag="VFZ86_06665"
     CDS             complement(19704..20462)
                     /locus_tag="VFZ86_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010622567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5642008.1"
                     /translation="MAHVLAFANQKGGVAKTTSTVNLGAALREHDLRVLAVDMDPQGN
                     LTMSQGIDTEGLDKSMYDVLVHRTPIEDVIYERELDVAASTIDLAGAEMALATMIGRE
                     RALQRALDAVRDKYDYILIDTPPSLGLLTINALTASEGVIVPVQCEYLSLRGLLQLEK
                     TLEMIRENLNPDVRIKGILPTLFDARTLHGRESIEVLRENFGDLVFDTVVRKTIKFAE
                     APVTGASVLKYDPRGRGAESYRALAREVLNGRSS"
     gene            complement(20604..21050)
                     /gene="yajC"
                     /locus_tag="VFZ86_06670"
     CDS             complement(20604..21050)
                     /gene="yajC"
                     /locus_tag="VFZ86_06670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEX5642009.1"
                     /translation="MSFTIIIWIVVFFGIFYFLAIRPQRRQKQQHVEMISMLKKGDEV
                     VTIGGMYGTVSAIGDDWVELEIAKRTRVRYLKRAISSITSIDEYDEDEEDEYIEAADD
                     EIIEEADEDVVYEEEVVEEAEIADDTAAEVAGETPDAPDAPEAPKA"
     gene            complement(21055..22176)
                     /gene="tgt"
                     /locus_tag="VFZ86_06675"
     CDS             complement(21055..22176)
                     /gene="tgt"
                     /locus_tag="VFZ86_06675"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEX5642010.1"
                     /translation="MEAEGFAFTVEARDGAARAGLLTTPHGVVETPCFMPVGTKATVK
                     AVEPRELRELGARIVLGNTYHLYLRPGTEIVAAHGGLHGFMQWDGPILTDSGGFQVFS
                     LEGTRLLRDDGVEFSSVYDGSRHVFTPELSMRVQEELGADVVMAFDQCAPGTLPRVEL
                     QAAVERTTRWAAACKAAKRRRDQLLVGIVQGGTDEDLRRRSAAEIMGVGFDAYAVGGL
                     SVGERGDEMLATVELMDDLLPADRLRYFMGIGDPVGILDVIARGVDVFDCVLPTRMAR
                     TGTALLRGGKRLNLRNARFAADLAPLEEGCDCYTCRTFSRAYLRHLVTQKEMLGAQLL
                     SLHNLRVLLRLTGDARRAIRAGRFAEFANDVAGRDSAGG"
     gene            complement(22179..23222)
                     /gene="queA"
                     /locus_tag="VFZ86_06680"
     CDS             complement(22179..23222)
                     /gene="queA"
                     /locus_tag="VFZ86_06680"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEX5642011.1"
                     /translation="MTLGDLDYVLPEELIAQTPAEPRDASRLLVARRAGGALEDRVFD
                     ELPSLLRAGDVLVRNDTRVFPARSFFHRATGGRIEVLFLYALTRPGDASERWEALLRG
                     RPRPEEVLESEVLGPDWPLRCIEPIGDGRWVVENLGGAPVLALLDEAGVTPLPPYITT
                     PLDDPERYQTTYSRSLGSAAAPTAGLHFTPALDDALAAADVAVEHVTLHVGLGTFKPL
                     QSDALHTTTLHSESFEVEAAVWARLLEARRDGRRIVAVGTTSMRVVEHLALEAPDSSG
                     GPLRGATRLFIRPGFAFRMADGLVTNFHLPRTSLLALVMAFCGVTETRAIYRHAVERR
                     YRFYSFGDAMLAL"
     gene            complement(23240..23815)
                     /locus_tag="VFZ86_06685"
     CDS             complement(23240..23815)
                     /locus_tag="VFZ86_06685"
                     /inference="COORDINATES: protein motif:HMM:NF014707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEX5642012.1"
                     /translation="MTDLLLHTCCGPCSTVAVPAWRERGVDPVGWFENPNIQPGAELE
                     RRRESMWRYSRAAGLELIVPEDGEEAPGWRSWRDGLADAAPDERCAACLGLRLGAAGK
                     AARDLGFSRFSTTLTVSPYQRHDLIISAGERAAGAHGVDFVYLDARGRFRDGYAESRR
                     LELYRQPYCGCAASKWEAWHARRERRARRTA"
     gene            complement(23851..24888)
                     /gene="ruvB"
                     /locus_tag="VFZ86_06690"
     CDS             complement(23851..24888)
                     /gene="ruvB"
                     /locus_tag="VFZ86_06690"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEX5642013.1"
                     /translation="MPRKPQDRIGDPNERPDDQDLELSLRPRRFADFVGQRVAKEQLE
                     IFVEAAKQRGDTLDHVLIAGPPGLGKTTLAGIIAQELGVGMHTTSGPALERKVDVAGL
                     LTNLQEGDVLFVDEIHRLNAAVEEVLYPAMEDYEIDIMIGEGPAARSIRMQVQPFTLI
                     GATTRTGLLTTPLRDRFGVWQRLEYYEIEELAAIVRRSAGILGVEVDGDSALEIAGRS
                     RGTPRVANRLLRRVRDFAQVRHEGVIDHDICLAALELFQVDGEGLDKLDRDILKIIAE
                     KFDGGPVGLDTLAVALGEEADTVMDVYEPYLIMQGFLKRTPRGRVLTRNGFLHCGLKP
                     PARGDTLFEDL"
     gene            complement(24888..25490)
                     /gene="ruvA"
                     /locus_tag="VFZ86_06695"
     CDS             complement(24888..25490)
                     /gene="ruvA"
                     /locus_tag="VFZ86_06695"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEX5642014.1"
                     /translation="MIASVRGRLALVDGERAVIEVGGLGLEVLASGRTLGSLVPHVGD
                     EVSLLTYLNVREDALQLFGFRDRGERTFFLWLTAVSGVGPKVALAVLSGYPVAELELA
                     VARDDVKKFESIPGIGKKLAQRLVVELRDKVGELPPVGAGAAGVTGAGVQADHFIEAR
                     SALQNLGLTLREAEEALRGAPEDAPLEELVKFALTHRAGE"
     gene            complement(25487..26002)
                     /gene="ruvC"
                     /locus_tag="VFZ86_06700"
     CDS             complement(25487..26002)
                     /gene="ruvC"
                     /locus_tag="VFZ86_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015845963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEX5642015.1"
                     /translation="MIVLGFDPGTASTGWGVIEQDGNRLHSLGHGCIVTSAKDPTHVR
                     LRQIYDQARGLIKRFCPDIVVLEELFVNVNVKTALAVGQAKGVLILAASEVEEAPCEY
                     SPLQIKMAVTGYGRASKIQVQEMTKTILCLPRIPQPDHAADALAVAICHTHHNSDGLR
                     AVRGIVGGRGT"
     gene            complement(26008..26757)
                     /locus_tag="VFZ86_06705"
     CDS             complement(26008..26757)
                     /locus_tag="VFZ86_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX5642016.1"
                     /translation="MSGHSKWATIKHKKGKTDARRGKLFSKLSRAITVAAREGGTDPT
                     MNMALANAIEKAKAESMPKDNIERAIQRGGGGAEGEQYESIVYEGYGPAGVAMIVEVL
                     TDNKNRSAAEVRNIFSKHGGQLAQPGAVAWGFERRGSITIDGSRFAEDDIMTAAIDAG
                     AEDVLQDGDEFQVLTEPSDFAAVRDAIIAAGIEIENADLTMIPKNTVKLEENDARKTM
                     KIMDALEDSDDVQEVYANFDIPEDVLEALAG"
     gene            complement(26777..27517)
                     /gene="pdxT"
                     /locus_tag="VFZ86_06710"
     CDS             complement(26777..27517)
                     /gene="pdxT"
                     /locus_tag="VFZ86_06710"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013423483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEX5642017.1"
                     /translation="MTPANGKRRLDWILSPDPALDPRGPGAGLVVGVLALQGDFREHR
                     VMLERLGARTLEVRRPEHLAGLHGLVVPGGESTTIHKLMAAYGLAEPIKAFAAAGGVV
                     YGTCAGLITAARETVEGTPPTLGLMDIVARRNAFGRQVRSFEADLEVRGLGPDPVHAV
                     FIRAPWIESAGPRVDVLATCRDRIVAAREGGVLVTAFHPELTDDTRLHELFLTMIRRR
                     QGEAAPAGATADSFAGEPGRAAAAAGHR"
     gene            complement(27531..28415)
                     /gene="pdxS"
                     /locus_tag="VFZ86_06715"
     CDS             complement(27531..28415)
                     /gene="pdxS"
                     /locus_tag="VFZ86_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006717695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEX5642018.1"
                     /translation="MTDIGTMRVKSGLADMLKGGVIMDVTNADQAKIAEDAGACAVMA
                     LERVPSDIRAQGGVARMADPTKIQEIQACVSIPVMAKARIGHFVEAQILQSLEVDYID
                     ESEVLTPADEANHINTWKFDVPFVCGAVNLGEALRRVSEGAAMIRTKGEAGTGDVVEA
                     VRHMRTIMGEIRRLQTLDEDELFVAAKQLPAPYDLVKWVAENGKLPVVNFSAGGIATP
                     ADAALMMQLGAEGVFVGSGIFKSEDPQSRANAIVKAATNYRDPAVLAEVSVGLGDAMP
                     GIDAARMPEEAKIQHRGW"
     gene            complement(28531..30606)
                     /gene="fusA"
                     /locus_tag="VFZ86_06720"
     CDS             complement(28531..30606)
                     /gene="fusA"
                     /locus_tag="VFZ86_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX5642019.1"
                     /translation="MNDPAKIRNIALVGHRGTGKTSLFEALIFDAGAVTRLGSVADGT
                     TISDWDDDEKKRQMSLSAGLAHVDRGGLSFNLIDTPGDSSFLADAIASLQVVETALVV
                     VNTVLGVEVQHERLWDRAAARGLGRVIVCNMLDRERADFDHALSLLQESFGAQVVAVQ
                     LPIGKEHDFSGVVDLLSMKAYTYQGGKPTVSDVPADLADAAAAARDKLIDAVASTNDD
                     LAEKYLMEEEITADELNAAFAAAVAAGQLYPVACASATANIAVDHIFDVLALTPSPAD
                     VAPPKALKGDEEVELVCDPSEPAVAFVFKTLADPFSGHVNVFRVFQGKMASDSQVVVA
                     RDGHKERMGQVLKTMGKESKAAPEALVAGDIGAIAKLKDVVTGDTLCADGNVVSFPAI
                     DFPSPLMSFAVTAKSKGEEDKVITALRRLSEEDPMLEVHHDDQTGEMIVGGMSQVHVE
                     VTVERMKRRFGVEVELKPPRVPYRETIKGTAKAEGKHKKQTGGRGQFADTWMEVRPQP
                     RGDGFEFENKIVGGAIPRNFIPAVEKGVRAAMDDGFLAGYPVVDVHVKLYDGKYHPVD
                     SSDMAFQIAGSLGFKAACEKAQPALLEPIMNVEITVPEENVGDIIGDLNSRRGRVLGT
                     TPRGHNNVVGAEVPLAEMLTYAPDLTSMTGGRGDYHMELLRYDEVPAHLTAKIVEQAQ
                     KDKEEAQKS"
     gene            complement(30721..>31529)
                     /locus_tag="VFZ86_06725"
     CDS             complement(30721..>31529)
                     /locus_tag="VFZ86_06725"
                     /inference="COORDINATES: protein motif:HMM:NF023227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642020.1"
                     /translation="GIGELTAADVHGRLRDLPVGDVCGIGPVTQERLSALGLTTVGML
                     QDVPFPLLAAAFGRGAHGLRQLALGRSFSPVRSERPLPKSAGREITFDDDVNDVELLR
                     ATLLSLTDSAVAELRRKGLAARTVALKVRFNTFHTVSRRRTLPRPVSAVRPLYETATA
                     LLDELDVGARWVRLVGVSVSGFTHNAFQLTLDDGWREVALGEAVDKVREKYGFRALTL
                     AGGAPARRHLDQASPAQSFHPASGAAAEAAAEASRELAAAALRDASSEPV"
BASE COUNT         4180 a        11272 c        11513 g         4564 t
ORIGIN      
        1 acgccagcgt gccgaagccg ctgcagggcg cgtccagcag cacggcgtcg aacgcctcgt
       61 cccactcagg ccgcgcctcc agggcgtcgg cctgcaccac ggcgatgtgg tccgcgccga
      121 ggcgcgcgag gttctcgcgc agctcgcccg cgcgatcctc gtccacctcg accgccgtga
      181 tcgcggcgcc cgcctgcagc gccgcgagct ggctggtctt ggcgccgggc gccgcgcaga
      241 ggtcgagcac ggccgcggcc ggcccggtca cggcgcccgc ggcgaccatc ccggcgatct
      301 gggagccccg cgactgcggc gtcacgagcc cctcccgaaa ggcccgcgac ctttcgagcg
      361 gcgggccctc gtacagcagc ccgtcgggca gcccgtcgat cccgcgcgtc gcgaacccct
      421 cggcggcgag cgcctcggcc gccgccggca cgcctccccg cagcctgttg gcgcgcaggc
      481 agcgttcggg ggcggcgttg gcggactcga gcagccggcg tgcggcggcg tcaccaagat
      541 cctcgcgcag cagcttcacg agccacgccg ggtacgagag accgaccgac cacgcacgca
      601 cggcgtctcc ctccccgagc tcctccagct tggcccggcc gtccgtcgcc acccgccgga
      661 gcacggcgtt gacgaacccc ccggcgcgac ggctcttgcg cgtggccagg gtgacggcgt
      721 cgtcgaccgc cgcgtaggcg ggcacccggt ccaggaacag gagctggaac gcggccagcc
      781 tgagcgcgtc gcgcacgtcc ggatccgcgg tcttgagcgg cgccttggtg taggcgccga
      841 gcaccgcgtc gatcgagccg cgccgcttga cggtcccgtt gaccagttcg ttggcgagcc
      901 cgcggtcggc ggggccgagt gaagagagct cgggcagcgc cgccgcggac tcgtcgagcc
      961 gcgccccggc gcgcagacgg cgcagcgccg cgaacgccgc ggcccgggca gggctcaccc
     1021 cggagcgtcc ggtcgtctcc cccgggccgg agccgctcac ggcgccagca gaccgcgtcc
     1081 ggcgccgcgc aggaactcgg ccgcggtcac gcggccgcgt ccctcctgct gcagctcgtg
     1141 gacgtccagc cagccctcgc cggcggcgac caccagccgg tcggggccgg tctccggcac
     1201 cgggccgtgg ccgggtgctg cgcgccagat gcgcgtccgc ctgccggcga gctcggtgac
     1261 cgcgccgatg tgcggcgaga gggcgcgcac ctggtcggcg atcgcggtgg ccggccggct
     1321 ccagtcgatc tccttgtcgc gcggcccgat cttggcggcg tacgttaccg ggccctcctg
     1381 ggcgcgccac tccacggtgc cggcggcgat gccgtcgagc gtcgccgcga cgccctccac
     1441 ggccgcgggc gccagcagtt cgtaggcgcg gccggcgtcg gcgtcgcggg gtacgtggac
     1501 gcgacgcgcg tcgccgatgg gcccctcgtc cacgccggcc gtcatccgca gcgtgcagac
     1561 gcccagctcg gtctcccccg ccatcagcgc ccgctccacg ggggcggcgc cgcgccagcg
     1621 cggcaccaga gacgggtgga cgacgatcgt ctccacggcg tccagcacgc gctgggcgac
     1681 gatctggccg taggcgcagg cgaccaggac gtcgggcttc gtttccagca gccgctccag
     1741 cgcctcggga tcggacagcc gctcgggctg gaacacgggg atgtggagcg cctcggccgt
     1801 cagcttgatg ggcggcgcct gcggcgtgcc gtgacggccg cgcgggcggt cgggattggt
     1861 gacgagcacg acgggcgtgc gctccgctcc ggcgaggccg tggagcacaa gctccgcgaa
     1921 cggcgccgtg ccggcgaagg cgagccgcat gcggctcaga ggccgccgcc ggccggctcg
     1981 atgccgccgg cctcgttcgc gcgcatggcc cgcagcgcgg cggcgcgcgc gctcctgctg
     2041 gtgcggtcga cgatgagcac gccgtcgagg tggtcgttct cgtgctggat cacgcgcgcc
     2101 tcgaacccct ccgcgcggaa gtccaccggc tcgccgtact cgtccagacc gcgcacacgt
     2161 aggctcacgg ggcgcgtgac gggcatggtc acgccgggga cgctgaggca gccctcgtcg
     2221 gcctcctcgg tctcttcgga gaactcgtcg agcacggggt tcgcgagcac ccgggggtcg
     2281 tcgtcgacgt cgtagaccag gaggcgctgg agcaccccga cctgcggcgc cgcgaggccc
     2341 acgcccgggg cgtccttcat ggtgcggatc atccgcttgg ccagcttgcg cagcccgcgg
     2401 tcgaactcgg tgacctcgcg cgcgtgctcg cgcagcacgg ggtcgccgat cacgcggatg
     2461 tccctgcggg cctcccgctg ccggcgcgag aggtcctgcg cgtccgcggt ctctctgatc
     2521 tgtgtcgtct cgctcatggt ctcaatggta cctcacaccg gggtcacagc cactgcggct
     2581 cgacgtccgg cacgacccgg acgccgcggg cggcgtagcg gtcgcggttg gtcgccagcc
     2641 aggcgcgcag cgcatcgagg gttttccctg tttccgtagt ctttatcacc acgcggcagg
     2701 cgtggcgacc gcgctcgcgc cagagcgggc cgggccccag cacctgggcc gtccccgcga
     2761 gcctcgcgcc cagccgctcc gcggtgaacc ggccggccac ctccaccttg tcccgcgagg
     2821 taccggagat gtcgaggccg acgaggaagc ccgccggcgg gtacccaagc tcccgccgcc
     2881 gctccagctc ctccgcgtag aactcctcct ccttgccggc cgccgcgagc gcgatcggcc
     2941 gcgcctgcgg gtcgagcgtc tgcacgatga cccgccccgg ccggtcgccg cggccgctgc
     3001 ggccgcccac ctggacgagc aaggcgaagg tccgctcctc ggcgcggaag tccgggaagt
     3061 gcagggtgag gtcggcgttg accacgccga ccagggtgac gtcggggaag tggtgcccct
     3121 tggcgatcat ctgggtgccc accagcacct tgggccccgg ctccgcgaag cggctcagga
     3181 caccctgcag gcgggcgtag gacccggcca cgtccgagtc caggcgcagc aggtccaccc
     3241 cgggcagcag gaggcggacc tcgcgctcca ggcgttcggt cccgaacccg tgacggacga
     3301 ggtcggagcg cccgcactcg gggcaggcgg acggcgccgg ttcggagtgg ccgcacgtgc
     3361 ggcagcgcag cgtgtggctg ccgaagagcg tgagcgtgac gtcgcagcgc gggcactccc
     3421 aggcgtggcc gcagtggtcg cagaccaggt acgacgcaaa gccgcggcgg ttgaggaaga
     3481 ggatggcctt ctcccccgcg tcgacgacgc cgctgagcgc ctcggcgagc ggcgcagaga
     3541 acacgccgtg gtgctcgcgc atgtcgacga cctccagctc gggcggccgc gagccgtcca
     3601 cgcgccggtg caggtcggcg tgctgcgcca cgcgggcgaa gctctccacg ctcggcgtcg
     3661 ccgaacccag cacgaccacg gcgccggtgc cctccgcccg ccagcgcgcg atcgtgcgcg
     3721 cgtcgtaggc cggttcgctc tcctgcttgt acgaggtgtc gtgctcctcg tccacgacga
     3781 tcagcccgag gtccacgagc ggcgcgaaca ccgccgagcg ggcgccgacg acgatgcgca
     3841 cctcgccgcg cgcgaccgcg ctgtaggcga ggagacgctc gccggccgag agaccggagt
     3901 gcaggaccgc gacctgcgcc ccggcgaacc ggcgcctgac gcgcgcgacc gtctgcccgg
     3961 tgaggccgat ctccggcacc aggagcagta ccgaccgccc ggtctccagc gtggcctgcg
     4021 ccgcctggag gtagacctcg gtcttgccgc tgccggtgac gccgtgcagc aggacctcgt
     4081 cgccggggcg cgtctcacac aggaggctgt gcagcgcgcg gcgctgctcg tcgagcaggt
     4141 ccgggacgtc ggagagggtg acgcaggcgc gcaggcgggc ggcctgcgcg gcggcgcgca
     4201 cggagacgcc cgcatccgcc tcggcgggcg agccgggccg gccgccgccc gtctcgtcgc
     4261 cggcgaccgc ggcgaagctc gccggcgggt cctccgcgag gagcgccccg gcggcgagca
     4321 ggcgtcgcag tcctgccgcc gagaggtcgc tggcggcccg gagctcgcgc tcgtcgaggc
     4381 tgccggcctc ctccacgatc tcgagcgcgg cgcgctgccg cgcgccgagc cgggacgggg
     4441 tctccgtacc ccggcgcagc atgcggcccg gcggcgtgac gccgacgacg cggacgcggt
     4501 aggcgacgcg cagccagccc ttgcgccggt accgctccgc atcggcggcc agtggtccgc
     4561 tgcgcaggtg caggccggcg agctccagga gctttgcctc gccggcctcc gcggccgcgt
     4621 ggccgccgtc cgtgagctcg taccgccgct cgatcttgag cgcgccggtg ggcgggcaga
     4681 cgagggcgag cgcggccgcg aaggagcaga gatagtgccg ctcgatgcgg cgggccacca
     4741 cgagcagttc ctcggggatg ggcgggacgc gcaggacgtc gcgcagcggc agcacgcgtc
     4801 cctcgtgcgc ggtgtggtcg ccgaccccga ggatcacccc gatgacggtc tgcgcgccca
     4861 gcggcgcggc gacgagcgcg ccgggcagcg cgccgccggc gagctcgggc gggatctcgt
     4921 agtcgaacgg ctccgagaac gcgcgggtgc ggacgagcgg gaagacggcg gcgtagggac
     4981 tcacgccgcg ccgccgtctc cccggcgccg cctcaggccg tgaggcccgc ggcggcgcgg
     5041 agcgcctctg ccttgtccgt gcgctcccac gtgaagtcgt ggtcgttgcg gccgaagtgc
     5101 ccgtaggcgg ccgtcttgcg gtagatggga cggcgcaggt cgaggtcgcg caggatggcg
     5161 gccgggcgca ggtcgaagtg ctcgccgatg agacgctcga tggtgacgag ctcgaccttc
     5221 tccgtgccgc cgcagtcgac catcaccgag acggggtggg cgacgccgat ggcgtaggcc
     5281 acctggacct ggcacttctc ggccatcccg gcggcgacga cgttcttggc cacgtagcgg
     5341 gcggcgtagg cgcccgagcg gtcgaccttg ctcggatcct tgcccgagaa ggcgccgccg
     5401 ccgtgcggag cggcgccgcc gtaggtgtcg acgacgatct tgcggccggt gaggccgcag
     5461 tcgcccatgg ggccgccgat gacgaacttg ccggtcgcgt tgatgacggt gatgtcctcg
     5521 agcttgccga cgtcgtacat gtgcttgggc atgatcggct cgacgacgtg ctcgaggagg
     5581 tcggggtaga tgatgctctg gaggtcggcg ctctcggcgt gctgcgtggc gagcacgatc
     5641 ttgacgatct cgaccggctt gccgtcctcg tagcggaccg tgacctggga cttgccgtcc
     5701 ggccgcaggt acggcaggat ctcggccttg cgcacgtcgg cgaggcgctt ggcgagcttg
     5761 tgcgccagca ggatcggcat gggcatgagc tcggcggtct cggtgcacgc gtacccgaac
     5821 atcatgccct ggtcgccggc gccctgcgtg tccagcgcgt cgtcgtcgtt gacgtcggtg
     5881 cgcacctcga gggcggtggt gacaccctgg ctgatgtccg gggactgctc gtcgatggcc
     5941 accatgacgc cgcaggtctc gtagtcgtag ccgtaatgcg cgctggtgta gccgacctcc
     6001 ttgaccgtct ggcgcacgat cttggggatg tcgacgtagg tctcggtggt gatctcgccg
     6061 gcgacgaagg cgaggccggt ggtgaccagc gtctcgcagg cgacgcgccc gtaggggtcg
     6121 tccttgagga tggcgtcgag gatggagtcc gagatctggt cggcgagctt gtcggggtgg
     6181 ccctcggtga ccgactccga cgtgaagagg tagttccgct tgtccaattg agtcctccgg
     6241 ggacacgcgg cacgggcaga gccggcgcgg ttggatgtgg tgatgcgggc gggtgctggg
     6301 cagcccgcgg gcccggggga gtctaccaga cggccgtgac ggccagcaac gcgccccgcc
     6361 taactcccgt ggcgttccct cttgggcgtg cgccccatga ggtcggcgac acactcctgc
     6421 ggcgtgcggt tgccctcgag cacctggtac acgttctccg tgacgggcat gtcgatgccg
     6481 aggcgggagg ccaggtcgtg gagggcgtag gcggtgtgca ggccctccac gacctggccc
     6541 acctcggcct ccaccgcggc cggcgaggct cccttggcca gcagctcgcc ggccagccgg
     6601 ttgcggctgt gccgcgacgt acaggtggcg atgaggtcgc cgatgccggc gaggccggtg
     6661 aaggtggcga tgtgcgcccc ctggatcatg cccagccggg tcatctccgc gatgctgcgc
     6721 gtgatgaggc tggcgcggga gttgtccccg aacccgaggc cgtcggacat gccgacggcc
     6781 acggcgatgg ggttcttgac cgcgccgcag aactccacgc cgacgacgtc gtcgttggtg
     6841 tacacgcgga aggtgtcgct ggtgatcgcc gcctggagcg cgagcgtctg cgcctcgtcg
     6901 gcggccgcga tcaccgtcgc cgagggcatg tccttgctga cctcctcggc gtggttgggg
     6961 ccggagagcg cggcggcgcc ggccccggtc tcctcctcga tcacctgcgt cgggcgcttc
     7021 agcgagccga tctcgtagcc cttggcgagg gagagctgga gcgcgcctgc cgcgcgccgg
     7081 ggcgccaccc acctcgccac ctcgcgcacg gcctgggttg gcaccgcgta gacgagcaat
     7141 ccggcgccgc tcaggtccgc gtcctccatc cccgagaagt cgatctccgc aggcaagcgc
     7201 acgcccggca ggaagtgcgg gttctcgtgc gtggcctgca gccgcgccgc ctcctcgcgc
     7261 gtcagcgcga ggacctcgac gtcgtgcccc cggcggacga gcaggcgcgc gaaggcactg
     7321 ccccagctgc cggaaccgat gaccgtcgcc ctcacgtcag gctcctccct gcccgtccgg
     7381 cgtcgcgtgc ccgccgctcg tccacggcag ccgcacgcgg tgctcctcgc cggccagcag
     7441 ccgctggatg ttgccgcggt gcgcccacca cacgatcacg gcgaccagca cgccggcgat
     7501 cttgtacggc accggctcgt ccagggagat ggtcagcacg ggcaccagga acgccgccgc
     7561 cagcgacgcc acgctgacgt accgcgtcgt gaggagcagg acgatccacg cgaccaggac
     7621 gatggcgaac accagcggca cgagcgcgag cacgacggcg gccccggtgg cgatgccctt
     7681 gccgccccgg cccttgagga agatcgagta catgtggccg accaccggag cggcggcgat
     7741 gaagatcgcg aaccacgggt cgaagagcgc cgccgcgagc gccgccggga tgtacccctt
     7801 gagcatgtca cagacgagca ccgccacgcc ggccttcttg ccgagcacgc ggaagacgtt
     7861 ggtcgtgccc acgttgcccg agccgtgccg gcgcacgtcc acgccgtaga agagcttgcc
     7921 cacgaccagg ccgaacggga tggaccctgc caggtaggcg agcacgaaca ggatcacgaa
     7981 gcccaggacc tggacgacga tcatcgcttg cccttgaagt cgatcacgag cggaacgccc
     8041 tcgagaccga actgcgcccg caggcggttc tcgacgtaga agccgaagtc acgcgtcacc
     8101 agcgtgcggt cgttgatctc gatggcgatg cgcggcggcg acgtgccgaa ctgggcgatg
     8161 tagtacatct tgaggcgctt gccgtgcttc tgcggcatcg cccggtcggc ggcgagctcg
     8221 gcgagcgcgc ggttgagcgc gcgcgtggag atgtgcgccg tgtagagcgc gccgagctcg
     8281 gcgaccttgg cgagcagctc cctgaccccg cgccgcgtct cggcggagac ggcgatcacc
     8341 ggtggccgct gacggagctt gcgcttggcg atgccggcga tctcctccag gtccggctcg
     8401 gcgatgtcgc acttgttcac ggcgatcacc gtggcgcagt tgcggtgcgt ggcctcggtg
     8461 cagatcgaga ggtcgagctc gccgaagcgc atggtggcgt cggcgacgat gatggcgacg
     8521 tgcgcgcggt ccaggctctg gacgctgcgc aggtacgcgt agtactccac gccgctcacc
     8581 ttggcggcct tgcgcatgcc ggccgtgtcg atgaagcgga agcggccggc gtcggtctcg
     8641 acgacgctgt cgatggcgtc gcgcgtggtg ccggcgatct cgctgacgat ggtgcgcttc
     8701 tcgcgcgcga tggcgttgaa cagcgacgac ttgccggcgt tgggccggcc gacgatggcc
     8761 accggcgtct cggccggctc ggcctcctcc tcaccgtctc cgcgctcggc tccggtcacc
     8821 tcgacgagac ggtcgagcaa ctcgccgctg ccggtgccgt gcaccgcgga gaagagcaag
     8881 ggctcgccga ggccgagcgc gtagatctcg ggcgcggtcg cggtgctggc cggatcgtcc
     8941 atcttgttgg cgaccacgat gaccggcgcc ttggcgcgac gcagcacgtc ggcgatggcg
     9001 tagtcgtcgg tcagcgggcc gtagcgaccg tcgaggacga agaggacgac gtcggcggcc
     9061 gcgatcgcgt gctcgacctg cccgcggatg tgccctgcga acggagcgtc ctcggtggtg
     9121 tcgtagccac cggtgtcgac gagcagcaga cgatgaccgg tccactccac ttcgatctcc
     9181 ttgcggtcac gcgtgacgcc cggggtctcg tggacgacgg tggcgcgagt gccggtgagg
     9241 cggttgacga gcgttgactt gccggcgttg ggtgagccga cgacggcgac tatggggagt
     9301 ggtttcatca ggacagtcta gcaaacgcgc cgcccagaga atcgccgacg ctgaggcggc
     9361 cgccgcacgc agcggacagg tcgtcctccc cgaggtcgtc gagggtgcgc cccagatcct
     9421 cgggcagcca ggagcgcggc gcgaggagcc actcacctcc ggcgagcggg tcgcgccgca
     9481 gcgcctccag cacctccgcg ccgccgagca gcccggtgac ggtgacgtgc gcgccgaaga
     9541 ggtggttctc gaccacgaag gcgcgcaccg gcacgcgggc ggcgggcgtg agggcggcgg
     9601 cggctcgcgc gaccacggga gccgcgagcg tccccgtgag caggcgcacg ccgctcacat
     9661 cgcggcgcgg gcgtcggcga ggtccctcgc cgtcgccgcc gtcgccccct cccgtccacg
     9721 ctgccgcgag cgcctccgcc tcgacgcgca gggccgccgc gatgccgacg ccgttctcgt
     9781 actggtcggg cgcgccgccg gccggcggct cctcgccggc gagcaggtag aactcgtcgg
     9841 cggcgtgcac gaacgcgtca ccccgccgct cgagaaagtc gcgctgccag ccgcggacgg
     9901 cggcgaccag cgcgcgcgct tcgccggccg tgacgcggcg caggtcgccc tccgcggcga
     9961 gcgagaccgg caccacgccg aggtcggccg cggcgtccag ggcgccggcg cgccgcacgg
    10021 tctcggcgag cacgtcgccg tcgttccagc cggggcagag cacgacctgc aggtgcagct
    10081 cgagtccggc cgcggcgagc cgctcgagca cggcccggga gctcgcggcc gcccgcccca
    10141 tgagacgcac gcgggcggcg tcgtcccagg cgtggagcga cacgtagagc ggcgccagcc
    10201 gcagctcctc gatccgctcg aggtcggcct cggtcaggtt ggtgagcgtc atgaagttgc
    10261 cgtgcaggaa cgacaggcgg tagtcgtcgt ccttgacgga cagcgccggc cggaggccgg
    10321 gcggcacctg gtcgacgaag cagaagcggc agcggttgcg gcaggtccgc gggtcgtcgc
    10381 ccagcccgcc ccggtcgagc gagatgccgt gccactcgcc acgctgcggc gcgaccacga
    10441 ggtccagcgg gtggccggcg cgcaggaccg tgagccacag cgtgccgtcg gcggccgcgt
    10501 cctccacgtc gaggacgtcg agcgggacgc ggccgttcag ggtgtcgatg cggtcgccgg
    10561 cgcgcagccc gccgcgggag gcggcgtccc tcgcgtcggc gaccacgacg ccgccgcagg
    10621 cctccccggc cgcctcaggc accgcagagg tccagcagcg cgcgcacggt ggcttcgatc
    10681 tcctcgtccg gggtcgaggc ccccgcggcg accccgatgc ggccggcgcc ctcgagccac
    10741 tcgggccgca gctccgccgc ggactcgatg tggtgggtcc gcggctcgat cgcctggcag
    10801 agctgcgcga gccgggccgt gttggcgctg ttgcggccgc cgaccacgac gacgacgtcc
    10861 acctcatcgg cgagctcgca ggcggcgctc tggcgtttct cggtggcgtc gcagacggtg
    10921 ttgaacacga gcagttcgcg agccaccggc gccagctcgg cggcgagcgc cgagaggttg
    10981 gcgcgggtct gtgtggtctg cacgaccacg cccacgcgct tgccgcgcaa gcgctcgagc
    11041 ggcagcccgg aggcgtcctc cacgaccacg gcgtcggctc ccgcgaagcc ttgcagcccg
    11101 accacctccg ggtggtcgcg ctcgcccagg atcaccaccg cgtagcccgc ctcgcgcagc
    11161 gccgccgcct tgcgctgcgc gaccgagacg aacgggcacg tcgcgtcgac cacgttgagg
    11221 cgccgcgcgc gcgcccgctc gatcacgtcc ggggggacgc cgtgggtgcg gacgatcagc
    11281 gtgccgaccg cggcgtcgtc gacctcgtcc acgacgtcga cgccgcggtc cttgaggctg
    11341 ccgaccacgc tcgggttgtg gatgatggga ccgagcgtgg ccacgggagc cggcgcgccc
    11401 tcgagcgcct cgcctgtgag gcgaagggcg cgctccaccc cgtagcagta gccggcgtag
    11461 cgggcgacga cgatctcgac cgccccggcg cgcacgtgcc gcagctctgc gctgcgttcc
    11521 ggctcctcat cgtaggacgc cgagccgtgc cccgcgacgg tggcgtcgtc agagtcgcgc
    11581 atacagtccc gtgacggcct cgtgcatgcg ctgcgcggcc tccctgtagg ccttgctgtt
    11641 ccgtgtcgtg atgtccgcca ggtcgaccgc cggcccgacg agcccgcgca gcgccggcag
    11701 gccgggcttg ccgccgcgga gcatgtactg ggtgccgtcc atcgccagcg ggatcacagg
    11761 ggcgccgctg cgcagggcga tcatgccgac gccgggcatg aactcgtgca ccgcgtcgtc
    11821 gcgctgccgg tgcccctcgg ggaacatcag caggatctca ccgcgctcga ggatcgtgag
    11881 cgcggtcttc agcgcctcgc ggtcgcccgc gccgcgatcc agggggaacg cgcccaggct
    11941 gccgatgaac ctcgcggccc acggattggc gaacagctcc ttcttggcca tgaaccgcag
    12001 cggccggtgg aagatcatgc cggcgatcac ggggtcccag aacgccttgt gggtcatgac
    12061 caccatcgcc ggtcccgtgc gcggcaggtg ctccgcgcca tgaacccggc agcggtagag
    12121 gccgccgaag aacgggatcg ccaaccaacg caggaacgtg tagagaccgg tgtctccgcg
    12181 tcctctgagc gggtaccccc acggcccggt cacgtgccca ccttctcgcg cacgacggcg
    12241 gcgacggcgt cgaccgcctg gtccagcgtc atctccgacg tgtcgacctc gacggcgtcg
    12301 ccggccatgc acagcggcgc gacctcgcgc tcgctgtcgc gccggtcccg ctcgacgagc
    12361 gcagcgagca cctcctcgac gtccacgtcc tcccccttcg cccgcagttc cgccgcacgg
    12421 cgcgccgccc gcacctccgg gcgcgccgtc aggaacacct tgacctcggc gccgggaaac
    12481 acgttggtgc cgatgtcgcg gccctccatg acgatggcgg cgccggaggc gagctctcgc
    12541 tgccgctcga ccagcagccg gcgcaccccc ggcagcaccg acacgaccga cgccgccgcc
    12601 cccatctcca gggtgcggat gaggccgctc acgtcgcgct cgcaggcgaa caggcgtccg
    12661 tcgccgtcga agcggagcgc cgcggcggcc gcgatcggca ccaccgccgc ctcgtcgtcc
    12721 agcgccactc ccgcctccgt cgccagcagg cccacggcgc ggtacatcgc gccggtgtcc
    12781 aggtacaggt acccgaggcg ctcggcgacg cgtcgcgcca ccgtgctctt gcccgagccc
    12841 acggggccgt cgatggccac gaccgtcact cggcgtcgac ctccctgcgg gtgctcacgt
    12901 cgtagccgag cacaccgagc acgtgcgtgg cccgccggca gacctcgcgc ccgagcacgt
    12961 acacggtgag cgtgccgccg agctccgggc tcatgtggtg catggcgagg tcctccacgt
    13021 tgatgccggc gtcgcccagc gcgaccatga tctcgctgaa cacgcccggg cggtcgggca
    13081 cggggatcat cacgcgatag agatcctcgg cggggaggct gccggcggcg agcatccgtt
    13141 cgcgctgctc cctggcgcgc gcgatggtgt cgctcaggcg cgtgtcgtcg ccggcggcga
    13201 gcgcctcgag cacctcctgc aggccctcct ggaactgcgc cagcgaggcg agcagcgccg
    13261 cccggttgtc catgaagatg tcggtccaca cgcgccggtt gctcccggcg atgcgggtga
    13321 ggtcgcggaa gctggggccg gccgagagca gggcgtcgcg cgccccggcg tgctggccgg
    13381 cctggctcat gagcacgttg gccagcacgt gcggcaggtg gctgaccacc gccatcacgc
    13441 ggtcgtgctc ctcggggtcg acggcgacgg gacgcgcgcc gatgtcgccg aggaacccgt
    13501 agagccgctg gtaggcgtcg gcgtccacgt ggtcgccggg cgtgacgaag taggtggcgc
    13561 cgtggtagag ggaggcgcgc gcgttggcga cgccggcggt ctccgagccg cagagcgggt
    13621 ggccgccgat gcagcgccgc tgctcggacg gagagagcgc gcgcatcagc ggccccttgg
    13681 tgctcccgac gtcgctcacc acggccgtcg gccccgcggc ggcgagcgcc cggcgcacgt
    13741 gcttcaccac gagccgcacg ggcgtgcaga cgaagatcag ctccgcgccc gcgcacgcct
    13801 cctccacgct tgcgcaagcc ttcgtgacgg ccccgcgctc gagcgccagc tcgagcgtgg
    13861 ccggcgtctg gctgaagccg cgcacgtggg ccaccccggc gcgttcgcgg gcggcgagcc
    13921 ccagcgaccc gcccatgagg ccgacgccga cgagcgcgac tgtgttcacg gctgcctcgg
    13981 ccacggcctc agacggactt gcccgtccag cgcgcgaggt ccaggacgcg cccggcgtac
    14041 tcgccgaaca ccgccgtcgg cagtgactgc ggaccgtcgc acagggcgtg ctcggggttg
    14101 gggtgcgtct cgacgagcac gccgtcggcg ccggcggcga cgccggccag cgacagcgga
    14161 agcaccaggt cgaggcggcc ggcggagtgg ctcgggtcga cgacgaccgg caggtggctg
    14221 gcgatcttga tcaccggcac ggcgctcacg tcgagcgtgt tccgggtggc cgtctcgaag
    14281 gtcctgatgc cgcgctcgca caggatgacg tcgcggttgc cctccttgac gatgtactcg
    14341 gccgccatga gcagctcctc gatcgtcgtc gcgggcccgc gcttgagcag caccggcttg
    14401 ccggccttgc ccagctcggc gagcagcagg aagttctggg tgttgcgcgc gcccacctgg
    14461 agcacgtcgg cgtaccggca gacgacgtcc aggtcgcggg ggtccacgac ctcggtgacg
    14521 atcggcagcc cggtctccgc gccggccgcc gccatcatcg ccaggccgtc ctctccgaga
    14581 ccctggaacg cgtagggcga ggtacgcggc ttgaacgcgc cgccgcgcat catgtggccg
    14641 cccgcttcct tgaccgccct cgccgccgcg agcacctgct ccggcgtctc gatggagcac
    14701 ggcccggcga tgagcgcgaa gtgccccccg ccgatcctgg cccccttgac gtcgatgacg
    14761 gtgtccttct gctggaactc acggctgacg agcttgtacg gccgcaggat cgcgacgacc
    14821 ctgtccacgc cgggcatggc ctcgagcggc agctgggtga tggcttcacg gtcgccgatg
    14881 accccgatga ccttgacgcg ctcaccggcc gagaggtgcg cgctggcgcc gatctgctcc
    14941 acccgcgcac agacgtgcgc cacctgttcg tcggtggccg cctcgtgcat gatcaccatc
    15001 atgctcacgg ccggctccct gcgccgggct ccccggggtc tgcctcgaga cacgccagct
    15061 tgtcgaggag gaactcgatc tcctcctgcg tgcccacggt cacccgcgcc cagccggggc
    15121 acccgagcgc gttgccgtcg cgcacgatcg cgcccatgga gagcagtgcg ccgcacacct
    15181 cgtcctgcgg cacgccgagg ccgtcgatgt ccacgagcat gaagttggcc tgcgagggga
    15241 cggtggcgcg gccacgcgcc gcgagccgct cgaccagcag gtcgcggagc ctcgcgttgg
    15301 tctcccggcg ctccgcgacc cggtccgggt gcttgagcgc ctcggtcgcg gcgagctggc
    15361 cgagcaggtt cacgttgaag ggctgccgca ccttgtcgag cgcctgcttg accgcggggg
    15421 cgcagatgcc gtacccggtg cgcagcccgc agagcccgta gatcttggag aacgtgcgca
    15481 ggacgaccac gttgtcgtgc gcctcctgga tctccagcga cccctcgcgg tcctcggccg
    15541 tgacgaactc gatgtaggcc tcgtcgagga cgaccacgac gtcgtcgggc acgcgctcga
    15601 cgaagcgcgc gacctcggcc gaggagacgt aggtcccggt cgggttgttc gggttgcaga
    15661 cgaagaccat cctggtgcgc ggcgtgacgg cggccgccat ggcgtccaga tcgtgggtgt
    15721 ggtccgccag gggcacggtc actgcgaccg cgccgcggat ggcgacgacg tcgcggtacg
    15781 aggtgaagga gggcgcggcg agcacgacct cgtcgccgcg ctcgagcgtg gcgtgcgcca
    15841 gcaggtacag cagttcgcag gtgccgcagc cgatggtgat ctgctcggga ctgcggtcgt
    15901 aggcggcggc cagcgactcg cgcaggtcgg tggagaatcc gtcggggtac cgctgcaggc
    15961 ggtcgagggc tcccatcacc gccgctttga cctccgggaa cggcggcagc gggaactcgt
    16021 tggaggcgag cttgaccacc tgctcgaggc cgaagcgccg ttggacggcc tcgatcggca
    16081 ggcccggctg gtacggggtg atgccggcca gctccgggat gaaacggacg gtcatgaggt
    16141 cactcctcgg cacgcatgca cggcgagtgc atgagtatac atatcatgcc tcgtcgcccg
    16201 gcggcggcgc gtccggctcc ccggacggct cggactccgc gtccggctcc ccggacggct
    16261 cggtcgcctc gtcttcctcc tcgagcgacg cgtcggcgac ggccgggacc tcctccgccg
    16321 ccagcatcag cgggaagccc tcgtcttcct tggctacctc gccggccacc tcttccccgg
    16381 cccggcgcag cgcctcgacc tcctcaccgg tgagctcgcg gtagccgccc ggctcgaggc
    16441 tcttgtcggt cagcggtccg tagcggcggc ggctgagctc ctccacgggc agcccgacgg
    16501 cgaggaacat ccgccgcacc tggcgcttgc ggccttcgtg gatggtgagc tcgacgaggg
    16561 ccgtcttggc gctctgcttc cggaggcgca cgtccgcggg cgcggtcacg ccgtcctcca
    16621 gatcgacccc ctggcggagc gtccccaggg cgcggcggct gaccttgccg cggacccgcg
    16681 cgacgtacgt cttgggcacg ccgtatctcg ggtgcatcag ccggttggcg agcacgccgt
    16741 cgttggtcag gatgatgaga cccgtggtgt cgacgtcgag gcggccgacc ggaaacaggg
    16801 cgcggccgcc gggcacgagg tccacgatga cgcggtccgc gtgggggtcc ctgcgcgtac
    16861 tcgcgacgcc ccgcggcttg ttgacgagca agtaccgcag ctcgcgcgcc gccaccggct
    16921 cgccgcgcac ggcgacgacg tcctccgcgg cgaccttctg gcgcgggaac gtcacgacct
    16981 cgtcgttcac ggtgaccgcg ccctcgcgga tgagctcgtc gcacgagcgg cgagaaccga
    17041 cgccggcgcg cgcgaggtac accaccaggt tcatgggctg gtcgggactc actgcgccct
    17101 cctctcggcg ttggcgtgca gccgcgagcg gatcgcgtcg acctcgtcgg cgcccaggga
    17161 gaactcctcc agcggcggca gatcggcgcg gctgccgagg ccgaaaagct tgaggaagcg
    17221 ctcgccggtg ccgtacagga tgggcgcgcc cggcgtgtcg gcgcggccct gctgttccac
    17281 caggccgcgt tcctcgagcg cgctgagcgc ccactcggac gagacgcccc ggacctcggc
    17341 gatctccgcc ctcgtcaccg gctcgaggta ggcgaccacg gcgagcgtct cgagaagggc
    17401 gggcgtgagg tcgtcgggct tctccgcgcc ggtgaaccgg cgcacgagct ccgcgcagtc
    17461 gtcggcgacg gcgaaggtga agcccccggc cacctcgcgc agctcgatgc cccgggcccc
    17521 ggcggcgtag tccgcgcgca gcgcgtccag cgccgtagcg gcttcaccct cgctgcaggc
    17581 gcaggcgtcg gcgagccgct cggcggcgac gggacgcgga gagatgaaca ggagcgcctc
    17641 gaggcgccgt gcgagtggaa gcatgctgag cccgggtcgg acggtgggac gcgtcgatgc
    17701 taccgcacgg cggcggccgg cgtcaccctg cggacggcgc cgccgcggcg atgcggctga
    17761 cgacgatgtc cccgaagtgc cgggactggc gcaatctcgc ttcgccgctc gagtgcagct
    17821 cgagcagggc gaagaaggtg acggcacgct ccaggcgtcc gcgcggcgcc accgcggcga
    17881 aggtgaactc accctcgtcg cccaggatga cccggaggcg ccgcagttcc ttgaccagcg
    17941 acacggcgag gtcggccaga tgactggtgt cggggaccgg cggctcgcgc agcagcgggc
    18001 cgagcgcctc ggcgagcgcc cgtgccggca aggcgccctc ggggatgcgc cggcggagca
    18061 gctccgccgg gaccggcgcg gtgcggtaga gccgggcgct gtgcgcctcc caggcggtgc
    18121 gcagcgcgtc ggcggcgttg cggaactggc tgtagcggac cagccgctcg agcagctgcc
    18181 gcgccgcctc gtcggggtcg aggtcctcga gctcgtcctc gacctcttcc tcgagcagca
    18241 ggcgcgactt catctcgagc agcgctgcca ccagcaccac gaactgcgag gtggcgtcca
    18301 ggtcgaactc gccactgtcc gccagcttga cgacgtactc ggcgatgatc cgcgagacgc
    18361 ggacctccca gatgtcgatc tcgtcacgca ggatgagtgt gacgagcagg tcgaacggcc
    18421 cgtcgaagac gtccaggtcg agctcgagct cgacgggagg tgcggcgacg gcacccgcat
    18481 cggcgcgcat cgccgtcgct gctccccggc tcattgacta ctgatcctcg ggctgattga
    18541 cgaacgacgg gatgtcgaag tccttgtcgg cgaaccgcgg cgcggggccg aacggcgtgc
    18601 cacggtcctc gcgcggctca ggtctgggcg ccggggtgcg ggcctccggc ccgcgggccg
    18661 gcgcctgcgc cggcctcacc tcgtcgccga acccggtggc gaccacggtg atggagatct
    18721 tgcccttgag cttctcgtcg acgaccgccc cgaagatgat gttggcgtcg tcgtcggcgg
    18781 cctcgctgat gacgccggcg gcctcgttga cctcgaacag cgtgaggtcc tcgccgccgg
    18841 tgatgttgag cagcaggccg cgcgcgccct cgatggtggt ctcgaggagc ggcgagtgga
    18901 tggccatgtc cgcggccgcc tgggccctgt tctcgccgcc ggccacgccg atgccgagca
    18961 gcgccgttcc cgcgtccttg acgatcgtgc gcacgtcggc gaagtcgagg ttgatgagcc
    19021 cggggaccgt gatgaggtcg gtgacgccct ggacgccctg gcgcaggatg tcgtcggcgt
    19081 gctggaacgc ctcgacgacg ctcgcgttgc gctccacgac gtcgagcagc cggtcgttgg
    19141 ggatggagat gaccgtgtcg gcgacctcgc gcagggcgtc gatgccttcc agcgcctggc
    19201 gctggcggcg gttgccctcg aacccgaacg gcctggtgac gatggcgatg gtcacggcgc
    19261 cgagctcgcg cgcgatctgc gcgacgacgg gagcgccgcc ggtgccggtg ccgccgccct
    19321 cgccggccgt gacgaacacg aggtcggcgc cgcgcagcgc agccttgagc tggtcgcggc
    19381 tgacctccat ggcctcgcgg ccgacctcgg ggttggcgcc tccgcccagg ccgtgcgtga
    19441 tctcctggcc gatgtggatc ttgacgtcgg cgtcgcacac ctcgagctgc tggacgtccg
    19501 tgttgacggc gatgaactcg acgcccttga cgcccgagtc gaccatgcgg ttcacggcgt
    19561 tggtgccggc cccgccgatg ccgaccacct tgatggtggt cacgtaagga cgacccttga
    19621 ctggagcgtc cgggcccgtg gggtcgggga ccgcaccgcc gctgtcggtg cgccggaaga
    19681 gctcgctcag tccatccttc atgctaggac gatctgccat tcagcacctc ccgcgcgagg
    19741 gcgcggtacg attcagcccc cctgcctctt ggatcgtact tcagcaccga ggccccggtc
    19801 acgggcgcct cggcgaactt gatggtcttg cgcacgacgg tgtcgaagac gaggtcgccg
    19861 aagttctccc gcaggacctc gatcgactcc ctgccgtgca gcgtgcgtgc gtcgaacagc
    19921 gtcggcagga tgcctttgat gcgcacgtcc gggttgaggt tctcgcggat catctccagg
    19981 gtcttctcga gctgcagcag gccgcgcagc gagaggtact cgcactgcac cggcacgatg
    20041 acgccctccg aggcggtcag cgcgttgatg gtgagcagcc cgagcgaggg cggcgtgtcg
    20101 atgaggatgt agtcgtactt gtcgcggacc gcgtcgagcg cccgttgcag ggcgcgctcc
    20161 cggccgatca tcgtcgccag cgccatctcg gcgccggcca ggtcgatggt ggacgcggcg
    20221 acgtccagct cacgctcgta gatgacgtcc tcgatgggcg tgcggtgcac gaggacgtcg
    20281 tacatgctct tgtcgagacc ctcggtgtcg atgccctgac tcatggtcag gttgccctgc
    20341 gggtccatgt cgaccgcgag cacgcgcagg tcgtgctcgc ggagggccgc gccgaggttc
    20401 accgtcgagg tggtcttggc caccccaccc ttctgattgg cgaacgcgag aacatgcgcc
    20461 atgtgagcgt tacccctgtg tcgagtccac gtcagaacgc agttcgggcc gcaggcaaga
    20521 atccctgcat gcgacgggcc ccgcagcgtc tgcggggccc gtcgtcacac gccgcgtctt
    20581 gaccgctgcc gaggccggcg acgtcacgcc ttgggagcct ccggggcgtc gggggcgtcg
    20641 ggcgtctcgc cggcgacttc agccgcggtg tcgtcggcga tctcggcctc ttcgacgacc
    20701 tcttcctcgt agacgacgtc ctcgtcggcc tcctcgatga tctcgtcgtc ggcggcctcg
    20761 atgtactcgt cctcttcgtc ctcgtcgtac tcgtcgatgc tggtgatgga ggagatggcg
    20821 cgcttgaggt accgcaccct ggtgcgcttg gcgatctcga gctcgaccca gtcgtcgccg
    20881 atggcgctga ccgtgccgta catgccaccg atggtgacga cttcgtcgcc cttcttgagc
    20941 atgctgatca tctcgacgtg ctgctgcttc tgacgacgct ggggccggat cgcgaggaag
    21001 tagaagatcc cgaagaagac gacaatccag atgatgatcg tgaacgacac ggactcaacc
    21061 tcctgcggag tctcgccccg cgacatcgtt ggcgaattct gcaaaacgcc cggcgcggat
    21121 ggcgcggcgg gcgtcgccgg tcaatcttag cagaacgcgc aggttgtgga gactcaggag
    21181 ctgcgcgccc agcatctcct tctgcgtgac cagatggcgc agatacgcgc gggagaacgt
    21241 gcggcaggtg tagcagtcac agccctcctc cagcggcgcc aggtcggcgg cgaagcgggc
    21301 gttgcgcagg ttcagccgct tgccgccgcg caggagcgcc gtgcccgtgc gggccatgcg
    21361 cgtcggcagc acgcagtcga acacgtcgac gccgcgggcg atgacgtcga ggatgcccac
    21421 ggggtcgccg atgcccatga agtagcgcag ccggtccgcc ggcagcaggt cgtccatgag
    21481 ctcgacggtg gcgagcatct cgtcgccgcg ctcgccgacg ctgagaccgc cgacggcgta
    21541 ggcgtcgaag ccgacgccca tgatctcggc ggccgaccgc cgccgcaggt cctcgtccgt
    21601 ccccccctgc acgatcccca cgaggagctg gtcgcggcgc cgcttcgccg ccttgcaggc
    21661 cgccgcccag cgcgtggtgc gctccacggc ggcctggagc tccacgcgcg gcagcgtgcc
    21721 cggcgcgcac tggtcgaacg ccatgacgac gtcggcgccg agctcctcct ggacgcgcat
    21781 cgacagctcg ggcgtgaaca cgtggcgcga gccgtcgtag accgagctga actccacccc
    21841 gtcgtcgcga aggaggcgtg tcccctccag gctgaacacc tggaacccgc ccgagtccgt
    21901 gaggatgggt ccgtcccact gcatgaagcc gtgcaggccg ccgtgggcgg cgacgatctc
    21961 cgtgccggga cgcaggtaca ggtggtaggt gttgccgagg acgatccgcg cgccgagctc
    22021 gcgcagctcg cggggctcca cggccttgac cgtcgccttg gtgcccaccg gcatgaagca
    22081 cggcgtctcg acgaccccgt gcggtgtggt gaggaggccg gcgcgagcgg ccccgtcgcg
    22141 agcctcgacg gtgaacgcga agccctccgc ctccatcgtc acagcgcgag catcgcgtcg
    22201 ccgaagctgt agaagcggta gcgccgctcg acggcgtggc ggtagatcgc ccgcgtctcc
    22261 gtcacgccgc agaacgccat gacgagcgcc aggaggctcg tgcgcggcag gtggaagttg
    22321 gtcaccagcc cgtccgccat gcggaacgcg aacccggggc ggatgaagag gcgggtggcg
    22381 ccccgcaagg gaccccctga ggagtccggc gcctcgagcg ccaggtgctc gacgacccgc
    22441 atcgacgtcg tgccgacggc gacgatgcgg cggccgtccc ggcgcgcctc gagcaggcgc
    22501 gcccacacgg ccgcctccac ctcgaacgac tcggagtgca gggtggtggt gtggagcgcg
    22561 tcggactgca gcggcttgaa ggtcccgagg ccgacgtgca gggtcacgtg ctcgacggcg
    22621 acgtccgccg ccgccagcgc gtcgtccagc gccggggtga agtgcaggcc ggccgtcggc
    22681 gccgccgccg agccaaggct gcgggagtag gtggtctggt accgctccgg gtcgtcgagc
    22741 ggcgtggtga tgtacggggg cagcggcgtc acgccggcct cgtcgagcag cgcgagcacc
    22801 ggcgccccgc cgaggttctc gacgacccag cgcccgtccc cgatgggctc gatgcagcgc
    22861 agcggccagt ccggcccgag gacttcgctc tccaagacct cctccggacg cggccgcccg
    22921 cgcaggagcg cctcccagcg ctcgctcgcg tcccccgggc gcgtgagcgc gtacaggaac
    22981 agcacctcga tacgcccccc ggtggcgcgg tggaagaagc tgcgcgcggg gaacacccgc
    23041 gtgtcgttgc gcacgagcac gtcgccggcg cggagcagcg acggcagctc gtcgaagacc
    23101 cggtcctcga gcgctccgcc cgcgcgccgc gcgaccagca gtcgcgaggc gtcgcgcggc
    23161 tcggccggtg tctgcgcgat cagctcctcg ggcagcacgt agtcgaggtc gccgagcgtg
    23221 atgcccgtgc ccgcgccgct cacgccgtcc tccgcgcgcg gcgctcccgg cgcgcgtgcc
    23281 acgcctccca cttgctggcg gcgcagccgc agtacggctg gcggtacagc tcgagccgcc
    23341 ggctctcggc gtagccgtcg cgaaagcgcc cgcgggcgtc cagatacacg aagtcgacgc
    23401 cgtgcgcgcc ggcagcccgt tctccggcag agatgatgag gtcgtgccgc tggtacggac
    23461 tgaccgtcag cgtggtcgag aagcgcgaga agccgaggtc gcgggcggcc ttgcccgccg
    23521 cgccgaggcg cagcccgagg caggcggcgc agcgctcgtc cggggcggcg tccgcgagcc
    23581 cgtcgcgcca cgagcgccac ccgggagcct cctccccgtc ctccgggacg atcagctcga
    23641 ggccggccgc ccggctgtac cgccacatgc tctcgcggcg gcgctcgagc tccgcgcccg
    23701 gctggatgtt ggggttctcg aaccagccca cgggatcgac cccgcgctcc cgccacgcgg
    23761 ggacggcgac ggtcgagcac gggccacagc aggtgtgcag gagcagatcg gtcatcgggc
    23821 gagtatagtc ggtgagcctg cgacgcagga tcacaggtct tcgaacagcg tgtcgccgcg
    23881 ggcgggcggc ttgagcccgc agtggaggaa gccgttgcgc gtgagcacgc ggccgcgcgg
    23941 cgtgcgcttg aggaagccct gcatgatcag gtacggctcg tagacgtcca tgacggtgtc
    24001 ggcctcctcg ccgagcgcga cggcgagcgt gtccaggccc accgggccgc cgtcgaactt
    24061 ctcggcgatg atcttgagga tgtcgcggtc gagcttgtcg aggccctcgc cgtccacctg
    24121 gaagagctcg agcgccgcga ggcagatgtc gtggtcgatg acgccctcgt gacggacctg
    24181 cgcgaagtcg cgcacgcgcc tcagcagccg gttggccacg cgcggcgtgc cgcgcgagcg
    24241 cccggcgatc tcgagcgcgg aatccccgtc gacctcgacc ccgaggatgc cggcggagcg
    24301 gcgcacgatc gccgccagct cctcgatctc gtagtactcg agccgctgcc agacgccgaa
    24361 gcggtcgcgc agcggcgtcg tcagcaggcc cgtccgggtg gtcgcgccga tgagcgtgaa
    24421 cggctggacc tgcatgcgga tgctgcgcgc ggccggcccc tcgccgatca tgatgtcgat
    24481 ctcgtagtcc tccatggccg gatagaggac ctcctcgacc gcggcgttga gccggtggat
    24541 ctcgtcgacg aagaggacgt cgccctcctg gaggttggtg agcaggccgg cgacgtcgac
    24601 cttgcgctcg agcgccggcc cggaggtggt gtgcatcccc accccgagct cctgggcgat
    24661 gatcccggcc agcgtcgtct tgccgaggcc gggcggcccg gcgatgagca cgtggtcgag
    24721 cgtgtcgccg cgctgcttgg ccgcctcgac gaagatctcg agctgctcct tggccacgcg
    24781 ctggccgacg aagtcggcga accggcgtgg cctgaggctg agctcgaggt cctggtcgtc
    24841 gggacgctcg ttggggtcgc cgatgcggtc ctgcggcttc ctgggcatca ttcacccgcc
    24901 cggtgcgtca gggcgaactt gacgagctcc tcgagcggcg cgtcctcggg ggcgccgcgc
    24961 agggcctcct cggcctcgcg cagcgtgagg ccgaggttct ggagcgcgga gcgggcctcg
    25021 atgaagtggt ccgcctggac gccggcgccg gtcacgccgg cagcgccggc gccgaccggc
    25081 ggcagctccc ccaccttgtc cctgagctcg accacgagac gctgcgcgag cttcttgccg
    25141 atgccgggga tgctctcgaa cttcttgacg tcgtcgcgcg ccaccgccag ctcgagctcg
    25201 gccacggggt acccggagag caccgccagg gcgaccttgg gcccgacgcc gctgaccgcc
    25261 gtgagccaga ggaagaacgt acgctcgccg cggtcgcgga acccgaacag ctggagcgcg
    25321 tcctcgcgca cgttgaggta ggtcagcagg ctgacctcgt cgccgacgtg cgggacgaga
    25381 ctgccgagcg tgcgacccga ggcgaggacc tcgaggccca gaccgccgac ctcgatcacg
    25441 gcgcgctcgc cgtcgacgag cgcgaggcgg ccgcggacgg aggcgatcac gtgcctctcc
    25501 cccccacgat gccgcgcacg gcgcgcaggc cgtcgctgtt gtggtgggtg tggcagatgg
    25561 ccacggcgag cgcgtcggcc gcgtggtccg gctgcgggat gcgcggcagg cacaggatgg
    25621 tcttggtcat ctcctgcacc tggatcttac tggcgcgccc gtacccggtc accgccatct
    25681 tgatctgcag cggcgagtac tcgcagggcg cctcttcgac ttcggacgcc gccaggatca
    25741 gcacgccctt cgcctggccg accgccagcg ccgtcttcac gttgacgttc acgaacaact
    25801 cctcgagcac cacgatgtcg gggcagaacc gcttgatgag cccgcgcgcc tggtcgtaga
    25861 tctggcgcag gcgcacgtgc gtcgggtcct tggcggacgt gacgatgcag ccgtgaccga
    25921 gcgagtgcag gcggttgccg tcctgctcga tgacccccca gccggtgctc gcggtgcccg
    25981 ggtcgaagcc caggacgatc atgctcgtca gcccgccagg gcttcgagga cgtcctcggg
    26041 gatgtcgaag ttcgcgtaga cctcctggac gtcgtcgctg tcttccagcg cgtccatgat
    26101 cttcatggtc ttgcgggcgt cgttctcctc gagcttgacc gtgttcttgg ggatcatggt
    26161 gagatccgcg ttctcgatct cgatgccggc cgcgatgatg gcgtcgcgca cggcggcgaa
    26221 gtcggacggc tcggtgagca cctggaactc gtcgccgtcc tgcaggacgt cctccgcgcc
    26281 ggcgtcgatg gcggccgtca tgatgtcgtc ctccgcgaag cgcgagccgt cgatcgtgat
    26341 cgacccgcgg cgctcgaacc cccacgccac ggcgcccggc tgcgccagct ggccgccgtg
    26401 cttgctgaag atgttgcgca cctcggcggc cgagcggttc ttgttgtcgg tgaggacctc
    26461 gacgatcatg gccacgcccg cggggccgta gccctcgtag acgatgctct cgtactgctc
    26521 gccctcggcg ccgcctccgc cgcgctggat ggcgcgctcg atgttgtcct tgggcatgga
    26581 ctcggccttg gccttctcga tcgcgttggc cagggccatg ttcatggtgg ggtcggtgcc
    26641 cccctcccgg gcggcgacgg tgatggcgcg cgagagtttg ctgaacagct tgccccgtct
    26701 ggcgtcggtc ttgcccttct tgtgcttgat ggtcgcccac ttggagtgcc cggacacggt
    26761 ggcaccacct cccttgtcag cggtggccgg ccgcggcggc ggcccggccg ggctcgccgg
    26821 cgaaggagtc ggccgtggcg cccgcgggcg cggcctcccc ctgccggcgg cggatcattg
    26881 tgaggaacag ctcgtgcaga cgggtgtcgt cggtgagctc ggggtggaac gcggtgacga
    26941 ggacgccgcc ctcgcgggcg gcgacgatgc gatcccggca cgtggcgagg acgtccaccc
    27001 gcggaccggc gctctcgatc cacggcgcgc ggatgaacac cgcgtggacg ggatccgggc
    27061 cgaggccgcg cacctcgagg tcggcctcga agctgcgtac ctgccgcccg aaggcgttgc
    27121 ggcgtgcgac gatgtccatg aggccgagcg tcggcggcgt gccctcgacg gtctcgcgcg
    27181 ccgccgtgat gaggccggcg caggtgccgt agacgacgcc gcccgcggcc gcgaaggctt
    27241 tgatgggctc tgcgagcccg taggcggcca tgagcttgtg gatggtcgtg ctctcgccgc
    27301 cggggacgac caggccgtgc agcccggcga gatgctcggg acgccgcacc tcgagcgtgc
    27361 gcgcgccgag gcgctcgagc atcacccggt gctcgcggaa gtcgccttgc agggcgagca
    27421 cgcccacgac gagtccggcg cccggaccgc gcgggtcgag cgccgggtct ggcgacagga
    27481 tccagtccag ccgccgcttg ccgttcgcag gtgtcacgct gcccccgcgc tcaccagccg
    27541 cggtgctgga tcttcgcctc ttcgggcatg cgcgccgcgt cgatgccggg catcgcgtcg
    27601 cccagaccca cgctcacctc ggcgagcacg gccgggtcgc ggtagttggt ggcggccttg
    27661 acgatggcgt tggcgcggct ctgcggatcc tcgctcttga agatgcccga gccgacgaag
    27721 acgccctcgg cgccgagctg catcatgagc gcggcgtcgg ccggcgtggc gatgccgccg
    27781 gcggagaagt tcacgaccgg cagcttgccg ttctcggcca cccacttgac caggtcgtac
    27841 ggcgccggca gctgcttggc ggcgacgaac agctcgtcct cgtccagcgt ctgcaggcgg
    27901 cggatctcgc ccatgatcgt gcgcatgtgg cgcacggcct cgacgacgtc gccggtgccg
    27961 gcctcgccct tggtgcggat catggccgcg ccctcgctga cgcgccgcag cgcctcgccg
    28021 aggttgacgg cgccgcagac gaacggcacg tcgaacttcc acgtgttgat gtggttggcc
    28081 tcgtcggcgg gggtgagcac ttcgctctcg tcgatgtagt cgacctcgag ggactgcagg
    28141 atctgcgcct cgacgaagtg gccgatgcgc gccttggcca tcaccgggat ggagacgcag
    28201 gcctggatct cctggatctt ggtggggtcg gccatgcgcg ccacgccgcc ctgggcgcgg
    28261 atgtctgacg gcacccgctc gagcgccatg acggcgcagg cgccggcgtc ctcggcgatc
    28321 ttcgcctggt cggcgttggt gacgtccatg atgacgccgc ccttgagcat gtcggcgagt
    28381 ccgctcttca ctcgcatggt gccgatgtca gtcaacgggg tacctccggt tgcttttgca
    28441 cgcaaatcct acctcagcgc gccaacgcca gcctaccggc gccacgccac caactgccca
    28501 gatttgcggg gaaaactgga cggattggac tcagctcttc tgcgcttctt ccttgtcctt
    28561 ctgcgcctgc tccacgatct ttgcggtgag gtgcgccggc acctcgtcgt agcgcaggag
    28621 ctccatgtgg tagtcgccgc gcccgccggt catggaggtg aggtccggcg cgtaggtgag
    28681 catctcggcc agcggcacct cggcgccgac gacgttgttg tggccgcgag gagtggtgcc
    28741 caggacccgg ccgcgccgcg agttgaggtc gccgatgatg tcgcccacgt tctcctcggg
    28801 cacggtgatc tcgacgttca tgatgggctc gagcagggcg ggctgggcct tctcgcaggc
    28861 ggccttgaag ccgagcgagc cggcgatctg gaacgccatg tcgctcgagt cgacggggtg
    28921 gtacttgccg tcgtagagct tgacgtggac gtcgacgacc gggtagccgg cgaggaaccc
    28981 gtcgtccatg gcggcgcgca cgcccttctc gaccgcgggg atgaagttgc gggggatcgc
    29041 gccgccgacg atcttgttct cgaactcgaa gccgtcgccg cgcggctgcg ggcggacctc
    29101 catccacgtg tcggcgaact ggccgcggcc tccggtctgc ttcttgtgct tgccttccgc
    29161 cttcgccgtg cccttgatgg tctcgcgata cgggacgcgc ggcggcttga gctcgacctc
    29221 gacgccgaag cggcgcttca tccgctccac cgtcacctcg acgtggacct ggctcatgcc
    29281 gccgacgatc atctctcccg tctggtcgtc gtggtggacc tcgagcatcg ggtcctcttc
    29341 gctgagacgg cgaagggcgg tgatgacctt gtcctcctcg cccttgctct tggccgtgac
    29401 cgcgaagctc atgagcggtg acgggaagtc gatggcgggg aagctcacca cgttgccgtc
    29461 ggcgcacagc gtgtcgccgg tcacgacgtc cttgagcttg gcgatggcgc cgatgtcgcc
    29521 ggccacgagc gcctcagggg cggccttgct ctccttgccc atcgtcttga gcacctggcc
    29581 catgcgctcc ttgtggccgt cgcgcgcgac caccacctgg gagtcgctgg ccatcttgcc
    29641 ctggaacacg cggaacacgt tgacgtggcc gctgaagggg tcggcgagcg tcttgaagac
    29701 gaacgcgacc gccggctccg aaggatcgca gacgagctcg acctcctcgt cgcccttgag
    29761 ggccttcggc ggggcgacgt cggccggcga cggggtgagc gcgaggacgt cgaagatgtg
    29821 gtccacggcg atgttggcgg tcgcggaggc gcaggcgacc gggtagagct ggccggcggc
    29881 gacggcggcg gcgaacgcgg cgttgagctc gtccgccgtg atctcctcct ccatgaggta
    29941 cttctcggcg aggtcgtcgt tggtgctcgc caccgcgtcg atgagcttgt cgcgcgccgc
    30001 ggcggccgcg tcggcgaggt cggcggggac gtcgctcacg gtgggcttgc cgccctggta
    30061 cgtgtacgcc ttcatgctga gcaggtcgac gacgccgctg aagtcgtgct ccttcccgat
    30121 gggcagctgc acggcgacga cctgggcgcc gaacgactcc tgcaggaggg acagggcgtg
    30181 gtcgaagtcg gcgcgctcgc ggtcgagcat gttgcagacg atgacgcgac cgaggccccg
    30241 ggcggcggcg cggtcccaga gccgctcgtg ctggacctcg accccgagca cggtgttgac
    30301 gacgacgagc gcggtctcga cgacctgcag gctggcgatg gcgtcggcga ggaagctgga
    30361 gtcgccgggc gtgtcgatga ggttgaacga gaggccgccg cggtcgacgt gcgccagacc
    30421 ggcggagagg ctcatctggc gcttcttctc gtcgtcgtcc cagtcggaga tggtggtgcc
    30481 gtcggccacc gagcccaggc gggtgaccgc gccggcgtcg aagatcagcg cctcgaacaa
    30541 cgacgtcttg ccggtaccgc ggtggccgac gagagcgatg ttcctgatct tggctgggtc
    30601 gttcatccgg ggtctcctcg ttgtcgttcg acggccgcct cccgcgccgg cgcgcgaggc
    30661 ggccgttcgt ctcctgtcgg aaggatgaag tctagcacag gggcggctgc ggcacgcggc
    30721 tcagacaggc tcggaagagg cgtcgcgcag ggccgcggcg gcgagctcgc gcgacgcctc
    30781 cgccgcagcc tcggcggccg cccccgaggc ggggtggaac gactgcgccg gagacgcctg
    30841 gtcgaggtgg cgcctggccg gcgcgccgcc cgccagggtg agcgccctga acccgtactt
    30901 ctcgcgcacc ttgtcgaccg cctcaccgag cgccacctcg cgccagccgt cgtccagggt
    30961 gagctggaag gcgttgtgcg tgaagccgct gacgctcacc ccgaccagac gcacccagcg
    31021 ggcgccgacg tcgagctcgt cgagcagcgc cgtggccgtc tcgtagagcg gccgcacggc
    31081 gctgaccggg cgcggcagcg tgcggcgccg gctcacggtg tggaaggtgt tgaagcgcac
    31141 cttgagcgcc acggtgcgcg ccgcgaggcc cttgcggcgc agctcggcga ccgcgctgtc
    31201 ggtgagcgag agcagcgtgg cgcgcagaag ctcgacgtcg ttgacgtcgt cgtcgaaggt
    31261 gatctcgcgg ccggccgact tgggcagcgg ccgctcgctg cgcaccgggg agaagctgcg
    31321 ccccagggcg agctggcgca acccgtgcgc gcccctgccg aacgccgccg ccagcagcgg
    31381 gaacgggacg tcctgcagca tgccgaccgt ggtgaggccg agggcggaga gccgctcctg
    31441 ggtcaccggc ccgataccgc agacgtcgcc gaccggcagg tcgcgcagac ggccgtggac
    31501 gtcggcggcg gtgagctcgc cgatgccgg
//
LOCUS       DASUMX010000138         5045 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1048126, whole genome shotgun sequence.
ACCESSION   DASUMX010000138 DASUMX010000000
VERSION     DASUMX010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 5045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5045
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1048126"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="VFZ86_06730"
     CDS             complement(<1..478)
                     /locus_tag="VFZ86_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEX5642021.1"
                     /translation="MAYAEERVRIAPPLDQPMSQDELDARVGQTITADGFGGIATMRL
                     FAEDLAPTCLSTDHPRYLSFIPCAPSDAAVMFDLVVGASSIYGGSWLEGAGAVYAENQ
                     ALRWIADLVGMPEGAGGVFTPGGTIGNLSALVAARHTARRTAAVGVRPNRVAATEGA"
     gene            complement(552..1373)
                     /locus_tag="VFZ86_06735"
     CDS             complement(552..1373)
                     /locus_tag="VFZ86_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX5642022.1"
                     /translation="MSSPASDPTAVPTAATSAALRADPTSPTAAPGRTVAPRSAAPGA
                     VPAVPAARNGSPRPRFETVLFDLDGTLADTIPLIVASYQHAFRTVLAEEVEEARARAW
                     IGRPLLPALLEEAPDHGHELDRVYREWNLANTARLIRRYPGVPEMLLALTRAGVRTAV
                     ATSKRRETARLALEGVGIDHLVDVVAGLEDTTRHKPDPDPLLHAAATLDVDPAECVYV
                     GDATVDVLAARAAGMAAVAVTWGAGERATLSATGPDAVVDTVPHLAAYLLGHREG"
     gene            1434..2921
                     /locus_tag="VFZ86_06740"
     CDS             1434..2921
                     /locus_tag="VFZ86_06740"
                     /inference="COORDINATES: protein motif:HMM:NF017416.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HEX5642023.1"
                     /translation="MNRAAWVRRGWVLLLEDRCVAYNFVVGDRDQLLLMPPSVRDWLP
                     AGHLAWFVLDVVAELDLSAFLARYRRDGRGGAAYDPQVMLAVLVYGYCVGERSSRRIE
                     QRLVEDVAFRVVAANQQPDHATIARFRAEHQDAIAGLFGQVLAVCSRAGVLRPGLVAI
                     DGTKLAANASMAANRTAEQLATQILAEAAATDAAEDAEDAAAKDGGQDPLGDLGSGVG
                     RRERLRAMLAELEDEAAQRSYEAHLARREEREAATGRALPGRRPSPTSSRHQSRRQAN
                     VTDPDSRLMKSRQGYLQGYNAQAVATIDQFVLAAQVTNVGVDAPSYPVMVGATKANLK
                     AAGERRRVRRVLADAGYWSKDNAALPAVESFIATGKRRQLRTIAETDTTRAEVIDAVQ
                     AGDLDIAQAAQQLGLAPAYVRQLLRRRRAGDPDTARTAMAAKLDTTQGRSTYRKRAAS
                     IEPVFAQVKHNRGIRSLSRRGLAAADSEWKLICATHNLLKLHRRR"
     gene            complement(3043..4422)
                     /locus_tag="VFZ86_06745"
     CDS             complement(3043..4422)
                     /locus_tag="VFZ86_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX5642024.1"
                     /translation="MIFTQYYLDCLSQASYLIADEGTGRAVVVDPRRDVAEYIDDARA
                     KGLTIVGVVNTHFHADFVSGHLELARETGAWIGYGEAAVADFPVRHLADGERISLGDV
                     TLEVMATPGHTPESISLLVFEHADDEVAHAVLTGDALFIGDVGRPDLLASVGVTADEL
                     GRMLYDSIQHKLMGLPDAVRVFPAHGAGSACGKNLSTETQSTIGEQRAFNYACQPMSE
                     DEFLAVVTEGQPSAPAYFIYNATLNKQERDVRDLDASVPALSATQVEAALASGAVVHD
                     AREVQEFAAAHLRGSVNVPADGRMAETVGMVFTPEQQVVVIAPEGVEQDVATRFARIG
                     FDHVVGYVADPEAFFLSHEDDVTRASRLTVAEADAAFARDDVQVVDIRNAGELETGGV
                     PGAAHIPLAELAARSAELDPTRPVVTYCAGGWRSSVAASLLRAKGFADVSDILGGYGA
                     WERAHATAS"
     gene            4553..>5045
                     /locus_tag="VFZ86_06750"
     CDS             4553..>5045
                     /locus_tag="VFZ86_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020489424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX5642025.1"
                     /translation="MTHLPDSATHALRASLPRRTSRTGRLVLVSALAVASIALAGCSS
                     GSDSEASVTAGPTAATAPASGAELDAASFAAALKLPGTTIIDVRTPAEFAEGHLPGAV
                     NIDIASPDFAAQVSTLDPGAPYAVYCRSGNRSASALAEMAAVGMTGAYHLGGGIGAWQ
                     SAGG"
BASE COUNT          708 a         1765 c         1883 g          689 t
ORIGIN      
        1 gcgcgccctc ggtggcggcc acgcggttcg gccgcacccc gaccgccgcg gtgcggcgcg
       61 cggtgtgccg ggcggccacg agggccgaga ggttcccgat cgtcccgccc ggggtgaaga
      121 cgccgccggc gccctccggc atccccacga ggtcggcgat ccagcgcagc gcctggttct
      181 cggcgtagac cgcccccgcg ccctcgagcc agctgccgcc gtagatcgac gaggcgccga
      241 cgacgaggtc gaacatcacc gccgcgtcgc tgggcgcgca cgggatgaag gagaggtacc
      301 gcgggtggtc ggtcgacagg caggtcggcg cgaggtcctc ggcgaacaac cgcatcgtcg
      361 cgatcccgcc gaacccgtcg gcggtgatcg tctgcccgac gcgggcgtcg agctcgtcct
      421 ggctcatcgg ctggtcgagc ggtggcgcga tgcgcacccg ctcctcggcg tacgccatga
      481 ccgcgtccgc gagtgccgcg agctcggtgt gcttgttctc gtggtaggtc ttcccgtgca
      541 cggcggcgac tctagccctc gcggtggccg agcaggtacg ccgcgaggtg ggggacggtg
      601 tcgacgacgg catccgggcc ggtcgcggac agggtcgccc gctcccccgc accccaggtg
      661 acggcgacgg cggccatgcc ggcggcgcgg gccgcgagga cgtcgacggt ggcgtcgccg
      721 acgtagacgc actctgccgg gtcgacgtcg agggtcgccg cggcgtgcag gagggggtcg
      781 ggatcgggct tgtggcgggt cgtgtcctcc aggcccgcga cgacgtcgac gaggtggtcg
      841 atgccgacgc cctcgagcgc cagccgcgcc gtctcgcgcc gcttggaggt ggcgaccgcg
      901 gtgcgcacgc cggcccgggt gagcgcgagg agcatctcgg gaacaccggg ataccggcgg
      961 atgagccggg cggtgttcgc gaggttccac tcgcggtaga cccggtcgag ctcgtggccg
     1021 tggtccgggg cctcctccag cagggcgggc aggagcgggc ggccgatcca cgcccgcgcc
     1081 cgggcctcct cgacctcctc ggcgagcacg gtgcggaagg cgtgctggta cgacgcgacg
     1141 atcagcggga tcgtgtcggc gagggtcccg tcgaggtcga acaggaccgt ctcgaagcgc
     1201 gggcgcggcg atccgttccg cgcagcgggg acggccggga cggcgccggg tgccgcgctg
     1261 cgaggagcga cggtccggcc gggcgccgcc gtgggcgagg tggggtccgc acgaagggca
     1321 gcgctggtgg cagccgtggg gaccgcagtg gggtcggatg ccggggagct caccggatca
     1381 gtctgcctcc caccggccgt gagtgcggaa acggtcgcca aggaggctgt cgcatgaatc
     1441 gtgcggcgtg ggtgcggcgg ggctgggtgt tgttgctcga ggatcgctgt gtggcttaca
     1501 acttcgttgt cggtgatcgt gatcagttgt tgttgatgcc gccctcggtg cgggactggt
     1561 tgccggcggg gcatctggcg tggttcgtgc tggatgtggt ggctgagctt gacctgtcgg
     1621 cgttcttggc gcggtatcgg cgtgatggtc gcggcggtgc ggcctatgac ccgcaggtga
     1681 tgctcgcggt gctggtgtac gggtactgcg tgggggagcg atccagccgg cggatcgagc
     1741 agcggctggt cgaggatgtc gcgttccggg tggtcgcggc caaccagcag cctgaccacg
     1801 cgacgatcgc gcggttccgc gcggagcatc aggacgcgat cgctgggctg ttcgggcagg
     1861 tgttggcggt gtgctcgcgc gccggggttc ttcgtccggg gctggttgcg atcgacggga
     1921 ccaagctggc cgccaacgcc tccatggcgg ccaaccgcac cgctgagcag ctggccacgc
     1981 agatcctggc cgaggccgcc gcgaccgacg ccgccgagga tgccgaggac gccgccgcca
     2041 aggacggtgg ccaggatccg ttgggggacc tgggctcggg cgtgggccgc cgggagcggc
     2101 tgcgggcgat gttggccgag ctggaggatg aggcggccca gcggtcctac gaggcgcacc
     2161 tggcccggcg cgaggagcgg gaggccgcca ccggtcgggc gttgcccggc cggcgcccgt
     2221 cgccgacctc gagcaggcac cagtcgcgaa ggcaggccaa cgtcaccgac ccggacagcc
     2281 ggctgatgaa gtcccgacag ggctacctgc agggatacaa cgctcaggcg gtcgccacga
     2341 tcgaccagtt cgtgctcgcc gcgcaggtca ccaacgtcgg cgtggacgcc ccctcgtacc
     2401 cggtgatggt gggcgcgacc aaggccaacc tcaaggccgc cggggaacga cgacgggttc
     2461 gcagggtcct ggccgacgcg ggctactgga gcaaggacaa cgccgccctg cccgcggtgg
     2521 agtccttcat cgccaccggc aaacgtcgcc agctgcgcac catcgccgag acagacacca
     2581 cgcgcgccga ggtcatcgac gccgtccaag ccggggacct cgacatcgcg caggccgcgc
     2641 agcagctcgg cttggccccc gcctatgtca gacagctcct caggcgtcga cgagccggcg
     2701 accccgacac ggcgcgcacc gcgatggccg ccaagctcga caccacccaa ggacgcagca
     2761 cctaccgcaa acgagccgcc agcatcgaac ccgtcttcgc ccaggtcaag cacaaccgcg
     2821 gcatccgatc actatcgcgc cgaggactgg ccgcagcgga cagcgagtgg aagctgatct
     2881 gcgccaccca caacctcctc aagctccacc gccggcgctg accccgcgca gcacccccgc
     2941 cgccgctgag ccgccgccga acccgccccc tccccgaaac acattcatgc gacagcctcc
     3001 agggcgacca cttccgcact cgcggcaaca atcctgggtg ggtcaggatg ccgtggcgtg
     3061 cgcccgctcc caggcgccgt agccgccgag gatgtcggag acgtcggcga agcccttggc
     3121 ccgcaggagg ctggcggcga cactgctgcg ccacccgccc gcgcagtagg tgacgaccgg
     3181 ccgcgtcggg tcgagctcgg cggagcgagc cgccagctcg gcgagcggga tgtgcgccgc
     3241 gccaggcaca ccacccgtct cgagctcacc cgcgttgcgg atgtcgacga cctgcacgtc
     3301 gtcgcgagcg aatgccgcat cggcctcggc gacggtcagc cggctggctc gcgtcacgtc
     3361 gtcctcgtgc gagaggaaga acgcctcggg gtcggcgacg tacccgacga cgtggtcgaa
     3421 gccgatccgg gcgaaccggg tcgcgacgtc ctgctcgacc ccctcggggg cgatgacgac
     3481 gacctgctgc tcgggggtga acaccatgcc gaccgtctcg gccatgcggc cgtcggcggg
     3541 gacgttgacc gacccgcgca ggtgcgccgc cgcgaactcc tgcacctcgc gggcgtcgtg
     3601 gacgaccgcg ccggacgcca gggccgcctc cacctgggtg gccgacaggg ccggcaccga
     3661 ggcgtcgagg tcacgcacgt cgcgctcctg cttgttgagc gtcgcgttgt agatgaagta
     3721 ggccggtgcg ctgggctggc cctccgtgac gacggcgagg aactcgtcct cggacatcgg
     3781 ctggcaggcg tagttgaagg cgcgctgctc accgatcgtc gactgggtct cggtcgagag
     3841 gttcttgccg cacgcggacc cggcgccgtg agccgggaag acgcgcacgg catccggcag
     3901 gcccatgagc ttgtgctgga tggagtcgta gagcatccgg ccgagctcgt cggcggtgac
     3961 gccgacggag gcgagcaggt cggggcggcc gacgtcgccg atgaacagcg cgtcgccggt
     4021 gagcacggcg tgggccacct cgtcgtcggc gtgctcgaag acgagcaggg agatcgactc
     4081 gggggtgtgg cccggggtcg ccatgacctc gagcgtcacg tcgccgagcg agatgcgctc
     4141 gccgtcggcg aggtggcgga ccgggaagtc ggcgacggcc gcctcgccgt agccgatcca
     4201 ggcaccggtc tcgcgggcga gctcgaggtg gccggacacg aagtcggcgt ggaagtgggt
     4261 gttgacgaca ccgacgatgg tcaggccctt ggcgcgggcg tcgtcgatgt actccgcgac
     4321 gtcgcgccgc gggtcgacga ccacggcccg gccggtgccc tcgtcggcga tgaggtacga
     4381 ggcctgggac aggcagtcga ggtagtactg ggtgaagatc acgtcaatcc tccaggggaa
     4441 atctcatggg tccatcgttg tcgaagtgct caacggatac cctagggggt atatttccgt
     4501 ggagcaagcc gctggagcaa gccacccgtt cgaccgaccc ctggagccct tcgtgaccca
     4561 cctgccagac tccgccaccc acgcgctgcg tgccagcctc ccgaggcgca cctcgcgcac
     4621 cggccgcctc gtcctcgtgt cggcactcgc ggtggcgtcc atcgcgctcg ccggctgctc
     4681 gagcggctcc gactccgagg cgtcggtcac cgccggcccg acggccgcca ccgccccggc
     4741 cagcggtgcc gagctcgacg cggcctcctt cgctgccgcc ctgaagctgc ccggcacgac
     4801 gatcattgac gtccgcaccc ctgccgagtt cgccgagggc cacctgccgg gggccgtcaa
     4861 catcgacatc gcgtctcccg acttcgccgc ccaggtctcg accctcgacc ccggcgcccc
     4921 gtacgccgtg tactgccgct cgggcaaccg ctcggccagc gcgctcgccg agatggcagc
     4981 ggtcgggatg accggcgcct accacctcgg tgggggcatc ggtgcctggc agagcgccgg
     5041 cggcg
//
LOCUS       DASUMX010000139         5058 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1336266, whole genome shotgun sequence.
ACCESSION   DASUMX010000139 DASUMX010000000
VERSION     DASUMX010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 5058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5058
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1336266"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="VFZ86_06755"
     CDS             <1..481
                     /locus_tag="VFZ86_06755"
                     /inference="COORDINATES: protein motif:HMM:NF016202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HEX5642026.1"
                     /translation="GICQYATTLFNAVFFAGLPVVEREAHSLYISHYPIGRDATVAWG
                     AVDFKFRNDTGRSVMIRSWIDGGALTVALVGKTGRTVTYDTSTFYDTRKPAHGKSDPR
                     VIYDDDLGPGVIRWEQGIDGRSVKVDRTVKDAAGKVLFRDSFVSRYEPMDWVKRVGT"
     gene            complement(531..1109)
                     /locus_tag="VFZ86_06760"
     CDS             complement(531..1109)
                     /locus_tag="VFZ86_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006976726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylation factor-like protein"
                     /protein_id="HEX5642027.1"
                     /translation="MPLINFAAREINYKIVYYGCGLCGKTTNLQYIHRKINPDARGKL
                     VSIATEEERTLYFDFLPLTLGTVKGMQTRFHLYTVPGQSYYNASRKLVLQGVDGVIFV
                     ADSQITRLDENVESYLNLWDNLLGQGDDLSKLGFVIQMNKRDLTDIFSVEDLNELLNH
                     TDSPVTESSAVTGQGVFETLKVVCKQVIAKTG"
     gene            complement(1119..1604)
                     /locus_tag="VFZ86_06765"
     CDS             complement(1119..1604)
                     /locus_tag="VFZ86_06765"
                     /inference="COORDINATES: protein motif:HMM:NF015232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HEX5642028.1"
                     /translation="MARRKGLVINDRQLDRCDDLLQKLKEVSGSSFLALISTSGQPIT
                     TASDEYHPETLSLASLAASSFAATQQLAKILDENEFTLLFHEGKGLNLHVSQVTDQVL
                     LIVTFGRETQVGKVRLYTQRAIEILRQIFERPEDEETPEMDAGFEAAAGTAMDDLFKD
                     M"
     gene            1825..2424
                     /locus_tag="VFZ86_06770"
     CDS             1825..2424
                     /locus_tag="VFZ86_06770"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HEX5642029.1"
                     /translation="MGLKGDLGELPLPDLVQMTSVGGKTGRLVLYDEEGAVAGVLAFR
                     GGRLIAAQSGELTAERAFYALLGLRTGSFDFDPAAELDAADVDLSTESLLMEGMRRLD
                     ETYRMRRRLPAPAVVRYVGGDTEDALEKRVLGYLGPGARTVGDVVEGLLVGGDADEYD
                     ALSALDHLAAGGTVRVDLPGEAGEADPQAGRPPQPELER"
     gene            2482..2769
                     /locus_tag="VFZ86_06775"
     CDS             2482..2769
                     /locus_tag="VFZ86_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642030.1"
                     /translation="MDDIVDLVTRSDDDLRALIVQLEAEEREISRRRRILHGELDILR
                     AELVMRLQRKHVQGESIVSADDVAGLSRILAGQGPVAGEPADADAPSEAEK"
     gene            2771..3232
                     /locus_tag="VFZ86_06780"
     CDS             2771..3232
                     /locus_tag="VFZ86_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX5642031.1"
                     /translation="MFCPECGSRNSEGASFCVRCGASLIADQPESATTLSYMPGEDDG
                     EITPFAACADDACLVIRSGGGRAGETYPLRDERVAIGRHPDAKIFLDDVTVSRNHAVV
                     VREGLAWVIVDEGSLNGTFVNRRRADRTVLSDGDEIQIGKYKLIFLAAAGS"
     gene            3249..3977
                     /locus_tag="VFZ86_06785"
     CDS             3249..3977
                     /locus_tag="VFZ86_06785"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX5642032.1"
                     /translation="MSDTAELLFDVGDERLLTIGSVVERLRGEFPDISVSKLRYLEEQ
                     GLVTPRRTKSGYRLYSQDDFSRLVRVLSMQRDEYLPLKVIRRELERSPAAALPSARQG
                     LRKTDLLSAGEGREYTAEEVMQLTGADAGLMDELEDYELIHGRQVSGVKRYSETDAGI
                     VAAAAQLAQLGLRPKNLRVIKSAVDREIGLIEQVLLPALRSNRPERRREGLDQLDDIV
                     QASTQLRQLLLARGVRRLTGGDSR"
     gene            4028..4555
                     /locus_tag="VFZ86_06790"
     CDS             4028..4555
                     /locus_tag="VFZ86_06790"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEX5642033.1"
                     /translation="MTDAGFDLAAHVRDIPDFPKEGILFRDIMPLLQDPRALRESVDR
                     LVEYGTGKHVDVVMGAEARGFILGAAVAYGLGAGFVCARKPGKLPHATISAEYDLEYG
                     TDKLEVHADAIQPGQNVLVHDDLLATGGTAKAKVELVEKLGGKVIGIAFIIELSGLEG
                     RKKLEGYDVFSLITY"
BASE COUNT          788 a         1725 c         1778 g          767 t
ORIGIN      
        1 cggcatctgc cagtacgcca ccacgctctt caacgccgtg ttcttcgccg gcctgccggt
       61 ggtggagcgc gaggcgcact cgctctacat ctcgcactac ccgatcggcc gcgacgccac
      121 cgtggcgtgg ggcgccgtgg acttcaagtt ccgcaacgac accggcagga gcgtcatgat
      181 ccgcagctgg atcgacggcg gcgccctgac ggtggcgctg gtggggaaga cggggcggac
      241 ggtcacctac gacaccagca ccttttacga tactcgtaag ccggcccacg gcaagtcgga
      301 cccgagggtg atctacgacg acgacctcgg ccccggcgtg atccgctggg agcagggcat
      361 cgacgggcgc agcgtcaagg tcgaccgcac ggtgaaggat gccgccggca aggtgctctt
      421 ccgcgacagc ttcgtctcgc ggtacgagcc gatggactgg gtcaagcgcg tcgggacctg
      481 acggcggcgc gctcccggcc ggcgggggag agctcccgcg ccgccggccg tcacccggtc
      541 ttggcgatga cctgcttgca gacgaccttg agcgtctcga agacgccctg ccccgtgacc
      601 gcgctgctct ccgtgaccgg cgagtcggtg tggttgagca gctcgttgag gtcctccacc
      661 gagaagatgt cggtgaggtc gcgcttgttc atctggatga cgaacccgag cttgctgagg
      721 tcgtcgccct ggccgagcag gttgtcccag aggttgaggt agctctcgac gttctcgtcc
      781 aggcgcgtga tctgcgagtc ggcgacgaag atgacgccgt cgacgccctg cagcacgagc
      841 ttgcggctcg cgttgtagta gctctgaccc ggcaccgtgt acaggtggaa ccgggtctgc
      901 atgcctttga cggtgccgag cgtgagcggg aggaagtcga agtagagcgt gcgctcctcc
      961 tcggtggcga tgctcaccag cttgccgcgc gcgtcggggt tgatcttgcg gtggatgtac
     1021 tggaggttgg tcgtcttgcc gcagaggccg cacccgtagt agacgatctt gtagttgatc
     1081 tcacgggcgg cgaagttgat caggggcata ggcgtgtctc acatgtcctt gaagaggtcg
     1141 tccatggcgg tgccggcggc cgcctcgaag ccggcgtcca tctccggcgt ctcctcgtcc
     1201 tccggcctct cgaagatctg ccgcaggatc tcgatggcgc gctgcgtgta gaggcgcacc
     1261 ttgccgacct gtgtctcacg gccgaacgtg acgatgagca ggacctggtc ggtgacctgg
     1321 gagacgtgca ggttgagccc cttgccctcg tggaagagca gggtgaactc gttctcgtcg
     1381 aggatcttcg ccagctgctg cgtggcggcg aaggagctgg cggcgagcga tgccagcgag
     1441 agggtctccg ggtggtactc gtcggaggcg gtggtgatcg gctgccccga ggtgctgatg
     1501 agcgccagga agctggagcc cgagacctcc ttgagcttct gcaggaggtc gtcgcagcgg
     1561 tccagctgcc ggtcgttgat gacgagtcct tttcgacgag ccacgcacgc acctcccgta
     1621 gacgcccgcg gttgccgaag tatatatcag cgccgccgcg ggcccgcgtc cgttggacgc
     1681 ttcccctcta tcgtcggtat cggggaaaag catgagtggc agccggagcc cgaagggcag
     1741 gaagccgggc gccggctgcg aagcttctga gcgtatggaa ggagacaaaa cgcgcatgat
     1801 cacaccgagc aggcgaggtc cgggatgggc ctgaagggcg acctcggcga actgccgctt
     1861 cccgacctcg tgcagatgac gtccgtgggc ggcaagaccg gccgcctcgt cctgtacgac
     1921 gaggaggggg cggtcgccgg cgtgctcgcg ttccgcggcg gccgcctgat cgccgcccag
     1981 agcggcgagc tcacggccga gcgggcgttc tatgcgctgc tcggcctcag gaccggctcc
     2041 ttcgacttcg acccggcggc cgagctcgac gcggccgacg tcgacctctc tacggagtcg
     2101 ctgctgatgg agggcatgcg ccgcctcgac gagacctacc gcatgcgccg gcgcctcccg
     2161 gcgccggccg tcgtgcgcta cgtcggcggg gatacggagg acgcgctcga gaagcgcgtg
     2221 ctcgggtatc tcggccccgg cgcgcgcacc gtcggcgacg tggtcgaggg cctgctcgtg
     2281 ggcggcgacg ccgacgagta cgacgcgctg agcgcgctgg accacctcgc ggccggcggg
     2341 acggtgcgcg tcgaccttcc cggggaggcc ggcgaggctg atccgcaggc cggccggcca
     2401 cctcaacctg agcttgaacg ttgatggccg gtccggtagc atcgacaatc cagccctcga
     2461 cgcacggaga ggagtcgcgc catggatgac atcgtcgatc tggtgacccg ttcggacgac
     2521 gacctgcgcg cgctcatcgt ccagctcgag gccgaagagc gcgagatctc gcgacggcgg
     2581 cgcatcctgc acggcgagct cgacattctg cgcgccgagc ttgtgatgcg cctgcagcgc
     2641 aagcacgtgc agggcgagtc catcgtctcg gccgacgacg tcgccggcct gtcgcggatc
     2701 ctcgcgggcc agggcccggt ggccggggag cccgcggacg ccgacgcgcc gagcgaggcg
     2761 gagaagtagg gtgttctgcc ccgagtgcgg cagccgcaac tccgaggggg ccagcttctg
     2821 cgtccgttgc ggggccagcc tgatcgccga tcagcccgag tcggcgacca cgctcagcta
     2881 catgcccgga gaggacgacg gcgagatcac gccgttcgcg gcctgcgccg acgacgcctg
     2941 tctcgtcatc cgcagcggcg gcggcagggc gggggagacc tacccgctca gggacgagcg
     3001 cgtcgccatc ggccgccacc cggacgccaa gatcttcctc gatgacgtca ccgtctcacg
     3061 caaccacgcc gtcgtcgtgc gcgagggcct cgcctgggtg atcgtcgacg agggcagcct
     3121 caacgggacg ttcgtgaacc gccgtcgcgc cgaccgcacc gtgctcagcg acggcgacga
     3181 gatccagatc ggcaaataca agctcatctt cctcgctgcg gcggggagct gagcggccgg
     3241 ggggcgacgt gtccgatacc gcggaactgc tgttcgacgt cggcgacgag cgcctgctca
     3301 ccatcgggtc ggtggtcgag cgcctgcgcg gcgagttccc cgacatcagc gtgagcaagc
     3361 tgcgctacct ggaggagcag gggctggtga cgccgcggcg caccaagtcc ggctatcgcc
     3421 tctactcgca ggacgacttc tcgaggctgg tgcgcgtgct gagcatgcag cgcgacgagt
     3481 acctcccgct caaggtcatc cgccgcgagc tggagcgcag ccctgccgcc gcgctcccgt
     3541 cggcccgcca gggcctgcgc aagaccgacc tcctgagcgc gggggagggg cgcgagtaca
     3601 ccgccgagga ggtcatgcag ctcaccggcg cggacgccgg cctgatggac gagctcgagg
     3661 actacgagct gatccacggc cgccaggtca gcggcgtcaa gcgctactcc gagaccgacg
     3721 ccggcatcgt ggcggcggcg gcgcaactgg cgcagctggg cctgcgcccc aagaacctcc
     3781 gcgtcatcaa gagcgccgtc gatcgcgaga tcgggctcat cgagcaggtc ctgctgccgg
     3841 cgcttcgctc caaccgtccc gagcggcggc gcgagggcct ggaccagctg gacgacatcg
     3901 tccaggcctc cacccagctc cgccagctcc tcctggcccg cggcgtccgc cggctcaccg
     3961 gcggcgacag ccgctaggcc tctgccggcc ggcagcgccg ccgcagtcgc acccacaagg
     4021 agatcccatg acagacgccg gcttcgacct cgccgcccac gtgcgcgaca tccccgactt
     4081 tcccaaggag gggatcctgt tccgcgacat catgccgctc ctgcaggacc cgcgtgcgct
     4141 gcgcgaatca gtggaccgcc tcgtggagta cggcaccggc aagcacgtcg acgtcgtgat
     4201 gggggcggag gcccgggggt tcatcctcgg cgccgccgtg gcctacggcc tcggcgccgg
     4261 gttcgtctgc gcgcgcaagc cgggcaagct cccgcacgcg accatctccg ccgagtacga
     4321 cctcgagtac ggcacggaca agctcgaggt gcacgccgac gccatccagc ccgggcagaa
     4381 cgtgctcgtg cacgacgacc tgctcgccac gggcggcacg gccaaggcga aggtcgagct
     4441 cgtcgagaag ctcggcggca aggtcatcgg catcgccttc atcatcgagc tctccggtct
     4501 cgagggccgc aagaagctcg agggctacga cgtcttcagc ctgatcacgt actgaccccg
     4561 aggagggcgg gcggcgcttg cgccggcccg ccgtcccgca tccgcgagcg cgattcgagc
     4621 gccgtcacga acatatgttc gtgtcggcgc tcgttcttgc ttctccgtgc gcgctaccgt
     4681 ctgggcgcca ggcccatccc ggcatttgca cgcccacatg cgcttcacgt tcatttggcc
     4741 gtcgactgtc gaagcctgac ctcggagctt cacagggcgt cggccaggcg cgtccgcgca
     4801 cgcggggagc agaaccctgg cggccaggtc agacgccggg cggacgcaag acgaccgtga
     4861 gggccggcgg tggcgcgcga tcgaatcgcc tgtagaacga gtgacaccgc caccgccgct
     4921 gcgccacgca ggatcgagac ctggggagag ccgggaaggg ccatcgccgg tccgcacagg
     4981 cgatcacagc cggccagacg ggaggacggg gtgatccaga ggccgcccgt cgcgtctcca
     5041 cacctagctt tacataac
//
LOCUS       DASUMX010000140         2530 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_43221, whole genome shotgun sequence.
ACCESSION   DASUMX010000140 DASUMX010000000
VERSION     DASUMX010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43221"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1062
                     /locus_tag="VFZ86_06795"
     CDS             <1..1062
                     /locus_tag="VFZ86_06795"
                     /inference="COORDINATES: protein motif:HMM:NF013878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEX5642034.1"
                     /translation="ILPLRGKIINVEKARIDKMLQNQEILTMISAIGAGIGEEFDVEQ
                     ARYHKIIIMTDADVDGSHIRTLILTFLFRHMRPLVDAGYVYIAQPPLYRLKQGNQETY
                     IHKEAQLEEILLRGRLEQIRVDNTDTGFTFSETKYERFLKLFGEYEGWVKKLKSQWGG
                     ELIDWLKNGPVIEAEITDFRALLAALREDIDKRYVIDVLSVDEEDQVADTRLVERRSG
                     LAANLRIPFAALAGREFQSLKRVHSRIAELVGRPPFTLHLGNRKSEAASYEELRVQIV
                     ELTKEGMQLQRFKGLGEMNPDQLWETTMNPMTRTLMLVSMEDAQAADEVFTMLMGDKV
                     EPRREFIEKNAKEVRFLDV"
     gene            1055..>2530
                     /locus_tag="VFZ86_06800"
     CDS             1055..>2530
                     /locus_tag="VFZ86_06800"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008419057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing)"
                     /protein_id="HEX5642035.1"
                     /translation="MSDTLQTLEGKIEPIELEDEMRSSFIDYAMSVLTDRALPDVRDG
                     LKPSQRRIMVAMNDLGLAPNKAHRKCAKIAGDTSGNYHPHGEAVIYPTLVRMAQDFNM
                     RYTLVDGQGNFGSVEGDSPAAMRYTEARFSKIAVEMLRDIDANTVDWMPNYDETRREP
                     KVLPSRMPNLLVNGAAGIAVGMATNIPPHNLGEVVDAIMHVIDNPEATADDLMGFIQG
                     PDFPTGGVILGTGGFKEAYRTGRGRVRVRAKAHTEQIKGNRSAIVVTELPYQVNRASL
                     IDNIVDLVKTKKITEISDLRNESDRSGMRLVIELKREAIPMVVLNKLYKHTQMQTTFG
                     IINIALVGGVPRTLTLPEMLKAYIAFQKEIVIRRTKFELDKAETRAHILEGLLVALDN
                     LDQIISIIRHAADAETARDALMDTFELTRPQAQAILDLRLQKLTGLERDKVKEEHAGL
                     LARIKELRDLLGDESAVLGVIKGELLEIRRLYNDDRRTEIVP"
BASE COUNT          527 a          881 c          771 g          351 t
ORIGIN      
        1 atcctgccgc tgcgcggcaa gatcatcaac gtcgagaagg cgcgcatcga caagatgctc
       61 cagaaccagg agatcctgac gatgatcagc gcgatcggcg ccggcatcgg cgaggagttc
      121 gacgtcgagc aggcgcgcta ccacaagatc atcatcatga ccgacgccga cgtagacggc
      181 tcgcacatcc gcacgctcat tctcaccttc ctcttccgtc acatgcggcc gctcgtcgac
      241 gcaggctacg tctacatcgc ccagccgccg ctgtaccggc tcaagcaggg caaccaggag
      301 acctacatcc acaaggaggc gcagctcgag gagatcctgc tgcgcggccg cctcgagcag
      361 atccgcgtgg acaacaccga cacgggcttc accttcagcg agaccaaata cgagcgcttc
      421 ctcaagctct tcggcgagta cgagggctgg gtcaagaagc tcaagagcca gtggggcggc
      481 gagctcatcg actggctcaa gaacggcccg gtgatcgagg ccgagatcac cgatttccgg
      541 gcgctcctcg cggcgctgcg cgaagacatc gacaagcgct acgtcatcga tgtcctcagc
      601 gtggacgagg aggaccaggt cgcggacacg cgcctcgtcg agcggcgcag cggtctggcc
      661 gcgaacctga ggatcccgtt cgcggccctc gccgggcgcg agttccagtc gctgaagcgc
      721 gtgcactcgc gcatcgcgga gctcgtcgga cgcccgccgt tcacgctgca cctcggcaac
      781 cgcaaatccg aggcggcgag ctacgaggag ctgcgcgtgc agatcgtcga gctcaccaag
      841 gagggcatgc agctacagcg cttcaagggc ctcggcgaga tgaacccgga ccagctctgg
      901 gagaccacca tgaaccccat gacacgcacg ctcatgctcg tgtcaatgga agacgcccag
      961 gcggcggacg aggtcttcac gatgctgatg ggcgacaagg tcgagccgcg tcgcgagttc
     1021 atcgagaaga acgcgaagga ggtgcggttc ctcgatgtct gacacgctgc agacgctgga
     1081 gggcaagatc gagcccatcg agctcgaaga cgagatgcgc tcgtcgttca tcgactacgc
     1141 gatgagcgtc ctcaccgacc gcgccctccc ggacgtgcgc gacgggctca agcccagcca
     1201 gcgccgcatc atggtcgcga tgaacgacct cggccttgcc ccgaacaagg cgcaccgcaa
     1261 gtgcgccaag atcgccggcg acaccagcgg caactaccac ccccacggcg aggccgtcat
     1321 ctacccgacg ctcgtgcgca tggcgcagga cttcaacatg cgctacacac tcgtcgacgg
     1381 tcagggcaac ttcggctctg tcgagggcga ctccccggcc gccatgcgct acaccgaggc
     1441 gcgcttcagc aagatcgccg tagagatgct gcgcgacatc gacgccaaca ccgtcgactg
     1501 gatgccgaac tacgacgaga cgcggcgaga gcccaaggtc ctgcccagcc gcatgcccaa
     1561 cctcttggtc aacggcgcgg cgggcatcgc cgtcggcatg gcgaccaata tcccgccgca
     1621 caacctcggc gaggtcgtcg acgccatcat gcacgtgatc gacaacccgg aggccactgc
     1681 cgacgacctc atggggttca tccaggggcc ggacttcccg accggcggcg tcatcctcgg
     1741 caccggcggc ttcaaggagg cgtaccgcac cgggcgcgga cgcgtccgcg tccgcgccaa
     1801 ggcgcacacc gagcagataa agggcaacag gagcgccatc gtcgtcaccg agctgccgta
     1861 ccaggtcaac cgggcatcgc tgatcgacaa catcgtcgat ctcgtgaaga ccaagaagat
     1921 caccgagatc agcgacctgc gcaacgaatc cgaccgctca ggcatgcgac tcgtgatcga
     1981 gctcaagcgc gaggccatcc cgatggtcgt gctcaacaag ctctacaagc acacccagat
     2041 gcagacgacc ttcgggatca tcaacatcgc cctggtcggc ggcgtgccgc gcacgctcac
     2101 cctgccggag atgctcaagg cgtacatcgc cttccagaaa gagatcgtca tccggcgcac
     2161 caagttcgag ctggacaagg ccgaaacgag ggcccacatc ctcgagggcc tgctggtcgc
     2221 cctggacaat ctcgaccaga tcatcagcat catccgccac gccgctgacg ccgagacggc
     2281 gcgggacgcg ctcatggaca ccttcgagct tacgcggccg caggcccagg ccatcctcga
     2341 cctgcggctc cagaagctca ccggcctcga gcgcgacaag gtcaaggagg agcacgccgg
     2401 cctcctggcg cgcatcaagg aactgcgcga tctgctcggc gacgagagcg ccgtcctcgg
     2461 cgtcatcaag ggcgagttgc tcgagatccg gcgcctctac aacgacgacc ggcgcaccga
     2521 gatcgtcccc
//
LOCUS       DASUMX010000141        11928 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1192926, whole genome shotgun sequence.
ACCESSION   DASUMX010000141 DASUMX010000000
VERSION     DASUMX010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 11928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11928
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1192926"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="VFZ86_06805"
     CDS             <1..249
                     /locus_tag="VFZ86_06805"
                     /EC_number="4.4.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020404197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphosulfolactate synthase"
                     /protein_id="HEX5642036.1"
                     /translation="RESGTVGIYRPTGDVKDGLLEEIRESFDITQLLFEAPIKKQQAW
                     FITQFGTNVNLGNIPPEEVISVETLRVGVRGDTLLTFH"
     gene            285..884
                     /locus_tag="VFZ86_06810"
     CDS             285..884
                     /locus_tag="VFZ86_06810"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX5642037.1"
                     /translation="MKLLLVRHCQTDWNRDPARCQGWAEVGLNEVGRAQARDLGRALE
                     GRGVELIVSSHLVRARETAEILHAELGGELPVVVDPRLAESHRGEWETRRFSSIVREE
                     SASWRHYRERPETFRFPGGESLEAQQRRVLACLRDCALDGRSALLVTHGGSIRLTRCF
                     LAGRGIDAFHETETVNGGVDEIETDGLVERIDISLTGKT"
     gene            888..1037
                     /locus_tag="VFZ86_06815"
     CDS             888..1037
                     /locus_tag="VFZ86_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642038.1"
                     /translation="MAERDNDRGHGDEERAASPDERTHPSPAGGPAAARAGRLTRDPV
                     CSSYG"
     gene            1194..2387
                     /locus_tag="VFZ86_06820"
     CDS             1194..2387
                     /locus_tag="VFZ86_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642039.1"
                     /translation="MTPTDDPTSTASDGAAAAEDRRPAPAKLKQRVIPDPPEMDQAQG
                     RRAFWFVLVLVLFTALVMGAVLALNVVADPYGSVGTHYFPTVTTSDRTVKADRIEQLE
                     GDPELVVLGSSRSMRYEPEYLEERTGLSAFNAGVNGIGGTADAWAMSQFIHDTRPEAD
                     PAYLWLLDVESFVPFEVGARTANEPRMAQYVGQASIGKGPREFARALWENRSTLFSLD
                     TARDSARLLLYREEAKSSQSKYRKQILADGVLKPRKWSEKEWKRRWPKSVERYSNLHK
                     NVYKGLDPTAQEYFEKTLAFMNEQGQTPVIALTPVNPKLRTILAPLGWTDRYEEVVAY
                     VESLQGEYDFVLLDMTDPRVFGFDKKEWYDGVHLTTVNTRRAIDYILEQTGGWPPSQP
                     PAGAR"
     gene            2398..3801
                     /locus_tag="VFZ86_06825"
     CDS             2398..3801
                     /locus_tag="VFZ86_06825"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HEX5642040.1"
                     /translation="MLLNSYTFLLLFLPVVVVLYWVIPRGYARLLFLVVASLVFYGLW
                     DWRYIPLLLATTLVDWVAGRSLARSEAEGASRRRKLILAAAVTVNLAFLGYFKYRGFF
                     VDQLDGILRLLGSGEPLPAVEVLLPIGISFYTFAGISYAVDVYRGTIKPARSVIHYLA
                     WVTLFPYILAGPIIRYGHVGAQLERAQQRFRWALIGTGLFFLVMGAAKKILVADMMAP
                     YVNELFSHHAQLGLWSGWAAALGYTLQLYFDFSGYSDMAVGVALMIGLRFPQNFDSPY
                     KAVNPSDFWRRWHMTLSGWLRDYLFIPLGGSRGTTLLTVRNLLITFLLGGLWHGPAWT
                     FVFWGLLWGFYQSVHVVARKYGLTPKWVWLNRILTFGAATVAWVFFRASSLRAAKNVF
                     EAMLGLQGVSGEGVRTVAPLLAAFIVGGLLWVNLLPNTWEIQPRPKLRYALLLGVLLA
                     ASLLALSKPSPFLYVQF"
     gene            complement(3832..4815)
                     /locus_tag="VFZ86_06830"
     CDS             complement(3832..4815)
                     /locus_tag="VFZ86_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642041.1"
                     /translation="MRVRVVQNRARAQVALVAVAALLLAAALTGALAPAAAAGPDAGA
                     LDTFLAQHASPMTGSGATFVREGQEHGVDPVFLVAIAGAETSFGAFLYAENGDQCTYN
                     AFNWFYGPTWPTSDFASWDEAIARVAQGLSGELYHAAGLVSVDAIAPKYCPDGTEQWV
                     ANVKAFMSELGGDWADTRIAATGVAPVPSPTPPSTEPGLVALSGSVKLDKGDREVGQR
                     IYAWFTLTNSGGQPLELGGIRLAIRGPGRVVRDMVSDQPLTLAAGQSLEVSSSWPLDL
                     AGRWHGWIEVVRDGKASLVGDEQAFGFWVRLPKDQVLQRWERRDATLSQSL"
     gene            4915..6036
                     /locus_tag="VFZ86_06835"
     CDS             4915..6036
                     /locus_tag="VFZ86_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642042.1"
                     /translation="MTGDLDRFACDARFPGLQAVRLIRRAIESTRLDLKGLRVLTEAS
                     VGYRRVTAVIAALAGADEVYAVGRDSVAASRKQAEEQTAYFAELARVGARVKLLSTRL
                     QAPLATVDVITDLPGVRPIDESIIRNVAGTAAVSLMRGTLHWRAGDLDVATCRRHGIA
                     VAGLDEEAVGLHRYAPQAVLAGLLDLGVEVTGATIVVAGDGPAVAYVVQALARLDARV
                     LVAAPETAGRVALYGGEKAGEVLGDEAVAGRLAEADALVLCPAGADARTVGPGTPVDA
                     ARLAAAAAHLAVVGLDAESDLRALGAAGLRCRPAGGPGGVFDLLPQAVIAQHAAGLKV
                     AEVMARARQRGSSPPVAEQLAAAEAHAELLPKDISVTRR"
     gene            6195..7886
                     /locus_tag="VFZ86_06840"
     CDS             6195..7886
                     /locus_tag="VFZ86_06840"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEX5642043.1"
                     /translation="MARRKKPGSHGYSLLLKDSVIYGAGRALQKFLVALLLPLYTAFL
                     SQADYGILGMVVTVTTFLDVFVTLGFDVAFSRFYFDDKSETARRRVITNVFYVSTVYP
                     LILLGTVGLLMPQLAPVLLGKEYDAGDWRYFAVALATLFFSNLNDLPFTLFRLEHRPW
                     IFSAYTIGRILIQVPLSIVFVAVFDWGPMGVLLANLFTAAGMQVGLLPTYIRKVDWRW
                     HWQLMRPMLAFAIPALFTGISFYWLKLSDRYFLLHYQGKAVVGLYTVAYSLSQPLYLT
                     LMAFRMAWPQWHYAKLHEPEKHRRMVSRSSTYFLALNAVMLVLMGAFMPFIVHTFLNE
                     RFWSIGPTTFVLALSVAVYAVYFVFWVGSNVAKKNRMIPVFFLIASAANIGLNFLLVP
                     EYGMYAAAWTHVAGFAILAVTVYFYSQKWYPIPYEWGRLLKIVLATVLTLAAAWGVGR
                     ALGQDVYMPYDELVLTTLAQVPTLLLFPLVLFATRFFTPGERERIGRALRRGRARRAA
                     VPAAATASGAAVAAAPVDRTRQLDQDDVEAEEEELEMEKEAEVDLLEGPSTTTIT"
     gene            7906..9120
                     /locus_tag="VFZ86_06845"
     CDS             7906..9120
                     /locus_tag="VFZ86_06845"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5642044.1"
                     /translation="MSPATGKKVLVVANPYPPMASAGTTRVVRFLRHLPDEGWEPSVL
                     TAAAEGPAAVPPGVRVARAAVPWPRQLLGGGKRSTRINRWVAVPDPYFAWVGPAVLLG
                     RRILARERFDVVMSSSPRASAHLVAAVLSEHAHVPWLADYRDPWSTYQFRRYPTRAHR
                     RAHEKLEAWALGRAAAVTAVNRPIVDDLVARHPWLDGRAHVLPNGFDRAEQPAEVALG
                     DGFWIVHTGRLYGREQQVAAFLEALATLPPEVKALFVGVDESRVRPDADRLGLGDRVR
                     VEPLVPLPVALGYQRAADALLLVNGRRPESMSSKVFEYLQAGRPVFAISPAGSAARAL
                     FAEVGGGTCVLPDDPMREPLAAFVAAARGGAAPLADPGALERYELGHLTAELAAILGD
                     LATDPRAPAPGA"
     gene            9200..10852
                     /locus_tag="VFZ86_06850"
     CDS             9200..10852
                     /locus_tag="VFZ86_06850"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX5642045.1"
                     /translation="MISSSSSDAGAGRGATGPAPGDGRRGPAPGGRFSAADAALLGGG
                     ALLTALVAAASTRSPLAAAALLVAIALAAAVILGRRRLARASVGAVLTALLVSLPVLA
                     LLGPSFALPVFPQAFLFRLVLAVVIYAGVCYLFVRRDPMPFAAKDLALPAVLWFAWLL
                     LGMIWAPDKLAALTYIAIVVTMAAVFLATAAAGGSRRRLIWFGYTMLAGYAFVVGFTI
                     LEARLGIRLPTSRLLTAVTSQTYAVTSVFHNQNDLATYIVLCWPFMLCAFFFTRRFKW
                     LALTLLFMAMGAAAFVRTGSRSSLVAAGISSLAAVVLFWHLGPKLSSRAGKVVMGLVI
                     VGLVVAAGWLLFNDSSNAMLRQFRLEALLSQAQSNQGSGAIRSNLTERGLEIAGGTWL
                     LGAGPGQAEGIIGSGTDALGISNLHNWWLETYADGGLVGFSLHVVFFLLLVVALWPVA
                     RHDPDPLTRYLACGTWLALLGWTIGSVGPSSSVGFAPMWILYGLGLAVVSRSRLATME
                     RETSLETGGPARPPGAPASIIAEAPGAPAKAASGGAGRAAAP"
     gene            10862..>11928
                     /locus_tag="VFZ86_06855"
     CDS             10862..>11928
                     /locus_tag="VFZ86_06855"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642046.1"
                     /translation="MRLDTFLKRAAERHPALVLQVSWANGLDIIRDLSAEGVPLLALD
                     ANPRALGLRSRLAAGSVCPDPQRDEEAFVSFLEDLGRRLPRKGVLFPTHDQYIWPVSK
                     HAERLEQWFLIPFSRWETMRRLHDKRAQMEAAWRVGVDTPKTVFVDSAADLERGVEEI
                     GFPCIFKPVESLAFKTRFRRHVLEIGSAEELHEVYAKVDDCGTLMLQDIVPGGDEELY
                     TLGSYLDAQSRPLAQFTGHKLRQHPPRFGHVSMAVSTWVPELAEAGLRLLHELGYHGV
                     SQVEFKRDPRDGRFRLMEVNARHWMWHSLAAACGVNLSLAAYRDAIGDPYITARQVDG
                     LRWVVSLTDARDAFSRWRRGE"
BASE COUNT         1574 a         4423 c         4116 g         1815 t
ORIGIN      
        1 cgcgagagcg ggaccgtggg catctaccgc ccgaccggcg acgtcaagga cggactgctc
       61 gaggagatcc gcgagtcgtt cgacatcacg caactcctct tcgaggcgcc gatcaagaag
      121 cagcaggcgt ggttcatcac gcagttcggc accaacgtca acctcggcaa catcccgccc
      181 gaggaagtca tctcggtgga gacgctgcgc gtcggcgtgc gcggcgacac gctgctcacc
      241 ttccactgac cgccgcggcg ccgcggcctc ggccaggaca gtcgatgaag ctcctcctcg
      301 tgcgccactg ccagaccgac tggaaccgcg accccgcgcg ctgccagggc tgggccgagg
      361 tgggactcaa cgaggtgggc cgcgcccagg cgcgcgacct cggtcgcgcg ctcgaggggc
      421 gcggcgtcga gctcatcgtc tccagccacc tcgtgcgcgc ccgggagacc gccgagatcc
      481 tgcacgcgga gctcggcggc gagctgccgg tcgtcgtcga cccgcgactc gccgagtcgc
      541 accgcggcga gtgggagacg cggcgcttct cgtccatcgt ccgcgaggag tccgcgagct
      601 ggcggcacta ccgcgagcgc ccggagacgt tccgctttcc cggcggcgag agcctcgagg
      661 cgcagcagcg ccgcgtcctc gcctgcctgc gcgactgcgc actggacgga cgctcggcgc
      721 tgctcgtcac gcacggcggc agcatccgcc tcacccgctg cttcctcgcg ggccgcggca
      781 tcgacgcctt ccacgagacc gagacggtca acggcggcgt cgacgagatc gagaccgacg
      841 gcctcgtcga gcgcatcgac atctccctga ccgggaagac ctgagccatg gccgaacgtg
      901 acaacgaccg ggggcacggc gacgaggagc gcgccgcctc gccggacgag cggacacacc
      961 cctcgcccgc gggcggcccc gccgcagcca gagcggggcg cctgacgcgc gaccccgtct
     1021 gcagcagcta cggctgagcg atcgtcgacg gcctcctcgg tctgtggagg atgcagcagc
     1081 gtttcaagcg gcccgacggc gggtgaggcg aacccgggag ggcgggctct cgtccctgtg
     1141 gtatccttcg gccccgtgcc gaccgctcat gcttttcgct gctcagagga ctcatgaccc
     1201 cgaccgacga ccccacttcg accgcgtctg acggcgccgc cgcggcggag gaccggcggc
     1261 cggctccggc gaagctcaag cagcgcgtca tccccgaccc gccggagatg gaccaggctc
     1321 agggccggcg ggccttctgg ttcgtgctcg tcctcgtgct cttcaccgcg ctcgtcatgg
     1381 gcgcggtgct cgcgctcaac gtcgtcgccg atccgtacgg ctccgtgggc acgcactact
     1441 tccccacggt cacgacgtcc gaccgcaccg tcaaagccga ccggatcgag caactcgagg
     1501 gagatccgga actcgtggtg ctcggcagct cgcgctcgat gcggtacgag cccgagtacc
     1561 tcgaagagag gacgggcctc agcgcgttca acgccggcgt caacggtatc ggcggcaccg
     1621 cggacgcgtg ggcgatgtcg cagttcatcc acgacacccg gcccgaggcg gaccccgcgt
     1681 atctctggct cctggacgtc gagtcgttcg tgccgttcga ggtcggcgcc cgcaccgcca
     1741 acgagccgcg catggcccag tacgtgggcc aggcgtcgat cggcaagggc ccgcgcgagt
     1801 tcgcccgcgc cctgtgggag aaccgctcca ccctgttctc cctggacact gcgcgagact
     1861 ccgcgcgcct gctgctctac cgcgaagagg ccaagagcag ccagagcaag tatcgcaagc
     1921 agatcctggc cgatggcgtg ctcaagccgc gcaagtggag cgagaaggag tggaagcgtc
     1981 gctggcccaa gtccgtcgag cggtacagca acctgcacaa gaacgtctac aagggcctcg
     2041 acccgacggc gcaggagtac ttcgagaaga ccctggcctt catgaacgag cagggccaga
     2101 cacccgtcat cgcgctcacg cccgtcaacc ccaagctgcg cacgatcctc gcccccctcg
     2161 ggtggaccga ccgctacgag gaagtcgtcg cctacgtcga gtcgctgcag ggcgagtacg
     2221 acttcgtcct cctcgacatg accgacccgc gcgtcttcgg gttcgacaag aaggagtggt
     2281 acgacggcgt ccacctgacc accgtcaaca cgcgccgggc gatcgactac atcctcgagc
     2341 agaccggcgg ctggccgccg tcgcagccgc cggcgggcgc gcggtaggcc cggcggcgtg
     2401 ctcctcaaca gctacacgtt cctgctgctc ttcctgccgg tcgtcgtcgt cctgtactgg
     2461 gtgatcccgc gcgggtacgc gcggctgctg ttcctcgtcg tcgcgtcgct cgtcttctac
     2521 gggctgtggg actggcgcta catcccgctg ctgctcgcga cgacgctcgt cgactgggtc
     2581 gcgggccgct ccctcgcgcg gagcgaggct gaaggggcga gccgccgccg caagctgatc
     2641 ctggcggcgg cggtcacggt gaacctcgcc ttcctcggct acttcaagta ccgcggcttc
     2701 ttcgtcgacc agctcgacgg catcctgcgg ctgctcggct ccggcgagcc gctgccggcg
     2761 gtcgaggtgc tgctgccgat cggcatcagc ttctacacct tcgccggcat ctcgtacgcc
     2821 gtcgacgtct accgcggcac gatcaagccg gccaggagcg tgatccacta cctggcctgg
     2881 gtcacgctct tcccgtacat cctcgccggc cccatcatcc ggtacgggca cgtgggcgcc
     2941 cagctcgagc gcgcgcagca gcgcttccgc tgggcgctca tcggcacggg cctgttcttc
     3001 ctcgtcatgg gtgcggccaa gaagatcctg gtggccgaca tgatggcgcc ctacgtcaac
     3061 gagctcttct cgcaccacgc ccagctcggg ctctggtcag gctgggcggc cgccctcggc
     3121 tacacgctgc agctctactt cgacttctcc ggctacagcg acatggccgt aggcgtcgcc
     3181 ctcatgatcg gcctgcgctt cccgcagaac ttcgattcgc cctacaaggc ggtcaacccg
     3241 tccgacttct ggcgccgctg gcacatgacg ctgtcgggat ggctgaggga ttacctgttc
     3301 atcccgctgg gcggctcgcg cggcacgacg ctgctcaccg tgcgcaacct gctcatcacc
     3361 ttcctgctcg gcggcctctg gcacggcccc gcctggacgt tcgtcttctg gggcctgctg
     3421 tggggcttct accagtcggt gcacgtggtc gccaggaagt acgggctcac gccgaagtgg
     3481 gtgtggctga accggatcct gacgttcggc gcggcgaccg tggcctgggt gttcttccgc
     3541 gcctcgtcgc tgcgcgccgc caagaacgtg ttcgaggcca tgctgggcct gcagggggtg
     3601 agcggcgagg gcgtccggac cgtcgcgccg ctcctggccg ccttcatcgt cggcggactc
     3661 ctgtgggtga acctgctgcc caacacgtgg gagatccagc cgcggcccaa gctccggtac
     3721 gcgctgctgc tgggtgtcct gctcgccgcg tcgctgctgg cgctgagcaa gcccagcccg
     3781 ttcctgtacg tgcagttctg atcacgccgg gccggagcgc ccgaagcggc gtcagaggga
     3841 ctggctgagc gtggcgtccc gccgctccca gcgctgcagc acctgatcct tcggcaaccg
     3901 cacccagaag ccgaacgcct gctcgtcgcc gacgagactg gccttgccgt cgcgcacgac
     3961 ctcgatccag ccgtgccagc ggccggcgag gtcgaggggc cacgaggacg acacctcgag
     4021 cgactgcccg gcggcgaggg tgagcggctg gtccgagacc atgtcgcgca ccacgcggcc
     4081 gggaccgcgg atcgccaggc ggatgccccc gagctcgagc ggctgcccgc cgctgttggt
     4141 cagggtgaac caggcgtaga tgcgctggcc gacctcacgg tcgcccttgt cgagcttgac
     4201 cgagccgctc agcgcgacga ggccgggctc ggtggacggc ggggtggggc tggggacggg
     4261 cgccacgccg gtggcggcga tgcgcgtgtc cgcccagtcg ccgccgagct cgctcatgaa
     4321 cgccttgacg ttggcgaccc actgctcggt gccgtcggga cagtacttgg gggcgatggc
     4381 gtcgaccgag acgagacccg cggcgtgata cagctcgccg ctcagcccct gggcgacgcg
     4441 ggcgatcgcc tcgtcccagg aggcgaaatc actggtgggc caggtggggc cgtagaacca
     4501 gttgaaggcg ttgtaggtgc actggtcgcc gttctccgcg tagaggaacg cgccgaagct
     4561 ggtctccgcg ccggcgatgg cgacgaggaa gacggggtcg acaccgtgct cctgaccctc
     4621 gcggacgaag gtggcgccgc tgccggtcat gggggacgcg tgctgggcga gaaaggtatc
     4681 gagggcgccg gcgtcgggac cagccgcagc cgccggcgcg agcgcgccag tcagtgcggc
     4741 ggccagcagc agcgcggcca cggccacgag ggcgacctgc gcgcgcgctc tgttctggac
     4801 gacccgcact ctcaccaggc tccccttgcc ggacaacgaa ggtcaagtat agccgggcga
     4861 gcgccggcgt gtggcgtagc gtccgcggcg cgacccagca ggtaggatga gaccatgacc
     4921 ggagacctcg accgcttcgc ctgcgacgcc cggttcccag ggctgcaggc ggtacgcctc
     4981 atccgccgcg cgatcgagag cacgcgcctc gacctcaagg gcctgcgcgt gctcacggag
     5041 gcgtcggtgg gctaccggcg cgtgacggcg gtcatcgccg ccctggccgg cgccgacgag
     5101 gtgtacgccg tcgggcgcga cagcgtggcc gcctcgcgca agcaggccga ggagcagacc
     5161 gcgtacttcg cggaactcgc gcgtgtgggc gcgcgcgtga agctcctctc gacacgcctg
     5221 caggcgccgc tcgccacggt ggacgtgatc accgatctgc cgggggtccg gcccatcgac
     5281 gagtccatca tccgcaacgt cgccgggacc gccgccgtct cactcatgcg cggcacgctg
     5341 cactggcgcg cgggcgacct ggacgtcgcg acctgcaggc gccacgggat cgccgtggcc
     5401 gggctggacg aggaggccgt gggcctgcac cgctacgccc cgcaggcggt gctcgccggg
     5461 ctgctcgacc tcggggtgga ggtgaccggc gccacgatcg tcgtggccgg cgacggcccc
     5521 gccgtcgcgt acgtcgtgca ggcgctcgcg cgcctcgacg cccgggtgct cgtggcggcg
     5581 ccggagacgg ccggccgggt cgccctgtac ggcggcgaga aggccggaga ggtgctcggc
     5641 gacgaggccg tggccggccg cctcgccgag gcggacgcgc tggtgctctg cccggcggga
     5701 gcggacgcgc ggaccgtcgg ccccggcacg ccggtggacg cggcgcgcct cgcggcggcc
     5761 gccgcgcacc tcgccgtggt ggggctggat gccgagagcg acctgcgggc gctcggcgcc
     5821 gccggcctgc gctgccggcc cgccggcggt cccggcggcg tgttcgacct gctgccgcag
     5881 gccgtgatcg cgcagcacgc cgccggcctc aaggtcgccg aggtgatggc gcgggcgcgg
     5941 cagcgcggct cgtcgccgcc ggtggcggag cagctcgccg ccgcggaagc ccacgccgag
     6001 ttgctgccca aggacatctc ggtcacgcgg cgctagcgcg ccgcctcgct cccgtacgtg
     6061 cgtcgggaag tccggcccga ggccggatgg tagaatcccg ccgaccacgg caaccggcgg
     6121 ccggcggccg tccccgcggc cggccgcgcc cacccagctc cggaggcacc acgaacccat
     6181 tcgccaggct gcgcatggcg aggcgcaaga agcccgggag ccacggctac tccctgctcc
     6241 tcaaggactc cgtgatctac ggggcggggc gcgcccttca gaagttcctc gtcgccctcc
     6301 tgctgccgct ctacaccgcc ttcctcagtc aggcggacta cggcatcctc ggcatggtcg
     6361 tcacggtcac gaccttcctc gacgtcttcg tcacgctggg cttcgacgtc gccttcagcc
     6421 gcttctactt cgacgacaag tcggagacgg cccgccggcg cgtcatcacc aacgtcttct
     6481 acgtctccac cgtctacccg ctgatcctgc tcggcactgt cggcctcctg atgccccagc
     6541 tggcgcccgt cctgctgggc aaggaatacg acgccggcga ctggcgctac ttcgccgtcg
     6601 cgctcgccac gctgttcttc agcaacctca acgacctgcc gttcacgctc ttccgcctcg
     6661 agcaccggcc gtggatcttc tccgcctaca ccatcggccg catcctcatc caggtgccgc
     6721 tctccatcgt cttcgtcgcc gtgttcgact ggggccccat gggcgtgctg ctcgccaacc
     6781 tcttcacggc ggcgggcatg caggtcgggc tgctgcccac ctacatccgc aaggtcgact
     6841 ggcgctggca ctggcagctc atgcggccga tgctggcgtt cgccatccct gccctgttca
     6901 caggcatctc gttctactgg ctcaagctct cggaccggta cttcctgctg cactaccagg
     6961 gcaaggcggt cgtcggcctg tacaccgtgg cgtactcgct gagccagccg ctgtacctca
     7021 cgctgatggc gttccgcatg gcctggccgc agtggcacta cgccaagctg cacgaaccgg
     7081 agaagcaccg caggatggtc tcgcgctcgt ccacgtactt cctcgcgctc aacgcggtca
     7141 tgctggtgct gatgggggcg ttcatgccgt tcatcgtgca cacgttcctc aacgagaggt
     7201 tctggagcat cgggccgacg acgttcgtgc tcgcgctctc cgtggccgtc tacgccgtgt
     7261 acttcgtctt ctgggtgggc tccaacgtgg ccaagaagaa ccgcatgatc ccggtgttct
     7321 tcctgatcgc ctcggcggcc aacatcggcc tcaacttcct gctggtgccc gagtacggga
     7381 tgtacgcggc ggcgtggacg cacgtggccg ggttcgcgat cctcgccgtg accgtctact
     7441 tctactcgca gaagtggtac ccgatcccct acgagtgggg ccggctgctc aagatcgtgc
     7501 tggcgaccgt gctgaccctc gccgccgcct ggggcgtcgg acgggcgctc ggacaggacg
     7561 tgtacatgcc ctacgacgag ctcgtgctga ccacgctcgc gcaggtgccg acgctgctgc
     7621 tgttcccact cgtgctgttc gcgacccggt tcttcacgcc gggggagcgg gagaggatcg
     7681 ggagggcgct gcgccgcggg cgcgcgcgcc gcgccgccgt gcccgccgcg gcgactgcga
     7741 gtggcgcggc cgtcgccgcg gcgcccgtcg accgcacgcg ccagctcgat caggacgacg
     7801 tcgaggcgga ggaggaggag ctcgagatgg agaaggaggc ggaggtcgac ctgctcgagg
     7861 gcccctcgac gaccaccatc acatgaacgc cctgaggacg acagcatgag cccggcgacc
     7921 ggcaagaagg tcctcgtggt cgccaacccg tacccgccga tggcgagcgc cggcaccacg
     7981 cgcgtggtgc gcttcctccg gcacctgccg gacgagggct gggagccgtc ggtgctgacg
     8041 gcagcagccg agggcccggc ggctgtgccg ccgggcgtcc gcgtggcgcg agccgccgtg
     8101 ccgtggccga ggcagctcct gggcggtggc aagcggagca ccaggatcaa ccgctgggtg
     8161 gcggtgcccg acccctactt cgcctgggtc gggccggcgg tactcctggg ccgcaggatc
     8221 ctcgcgcgcg agcgcttcga cgtcgtcatg agcagctcgc cgcgcgcgag cgcgcacctc
     8281 gtcgccgccg tgctctcgga gcacgcgcac gtcccgtggc tggccgacta ccgcgacccc
     8341 tggtcgacgt accagttccg ccggtacccg acgcgtgcgc acaggcgcgc ccacgagaag
     8401 ctcgaggcgt gggcgctcgg ccgcgccgcc gccgtcacgg cggtcaaccg cccgatcgtc
     8461 gacgacctcg tcgcgcggca cccgtggctg gacggccgcg cgcacgtgct ccccaacggg
     8521 ttcgaccgcg ccgagcagcc ggccgaggtc gcgctcggcg acggcttctg gatcgtccac
     8581 accggccggc tctacgggcg cgagcagcag gtcgcagcct tcctcgaggc gctcgcgacg
     8641 ctgccgccgg aggtcaaggc gctgttcgtc ggcgtcgacg agagccgggt gcgccccgac
     8701 gccgaccggc tgggcctcgg cgaccgcgtc cgcgtcgagc cgctggtgcc gctgccggtg
     8761 gcgctcggct accagcgcgc cgccgacgcg ctcctgctgg tcaacgggcg ccggccggag
     8821 agcatgtcga gcaaggtgtt cgagtacctg caggccggcc ggcccgtctt cgccatctcg
     8881 ccggccggct ccgccgcgcg cgctctcttc gccgaggtcg gcggcggcac gtgcgtgctc
     8941 cccgacgacc cgatgcgcga gccgctcgcc gccttcgtcg ccgccgcgcg cggcggcgcc
     9001 gcaccgcttg cggaccccgg agcgctggag cgctacgagc tcggccacct gacagccgag
     9061 ctggccgcga tcctcggcga ccttgccacg gacccccggg ctccggctcc gggcgcttga
     9121 cctgctcgaa gagatcaagg ggcagtcggg ggcacgcgcc gcctgagccc ctcgcagagc
     9181 tcgctggtag gattcggaca tgatctcgag ctcctcgtca gacgccggcg ccggtcgcgg
     9241 ggccacgggc cccgccccgg gggacggccg ccggggccca gcgcccggcg gcaggttctc
     9301 cgccgccgac gccgcgctgc tcggcggcgg cgccctcctc accgcgctgg tcgcggcggc
     9361 ctcgacgcgc agccctctcg ccgccgccgc cctgctcgtc gccatcgcgc tcgcggcggc
     9421 ggtgatcctc ggccgcaggc gcctcgcgcg ggcctccgtc ggcgccgtgc tcacggcgct
     9481 gctcgtctcc ctcccggtgc tcgcgctgct cggcccgtcg ttcgccctgc ccgtgttccc
     9541 gcaggcgttc ctcttccgtc tcgtgctcgc cgtcgtgatc tacgccggcg tctgctacct
     9601 gttcgtgcgc cgcgacccca tgccgttcgc cgccaaggac ctcgctctgc cggccgtgct
     9661 ctggttcgcc tggctgctgc tcgggatgat ctgggcgccg gacaagctcg cggcgctcac
     9721 ctacatcgcc atcgtggtca cgatggccgc cgtgttcctc gcgacggcgg cggccggcgg
     9781 ctcccgccgg cggctcatct ggttcggcta cacgatgctg gccggctacg ccttcgtcgt
     9841 cggcttcacc atcctcgagg cgcgcctcgg catccgcctg cccacgtcgc gcctgctgac
     9901 ggcggtgacc tcccagacct acgcggtcac cagcgtgttc cacaaccaga acgacctggc
     9961 cacctacatc gtcctctgct ggccgttcat gctctgcgcg ttcttcttca cgcgacggtt
    10021 caagtggctg gcgctcacgc ttctcttcat ggccatgggc gccgccgcgt tcgtgcgcac
    10081 cggctcgcgc tcgagcctgg tcgccgcggg tatctcgtcg ctggccgccg tcgtcctctt
    10141 ctggcacctc gggccgaagc tctccagccg ggccggcaag gtcgtgatgg ggctcgtgat
    10201 cgtcggcctc gtcgtcgcgg cgggctggtt gctcttcaac gacagctcca acgccatgct
    10261 gcggcagttc cgcctggagg cgctgctgag ccaggcgcag tccaaccagg gctcgggagc
    10321 catccgctcc aatctgacgg agcgcggcct ggagatcgcg ggcggcacct ggctcctggg
    10381 cgccggcccg ggccaggccg agggcatcat cggctccggc accgacgcgc tcggcatcag
    10441 caacctgcac aactggtggc tggagacgta cgccgacggc gggctcgtcg gcttctcgct
    10501 gcacgtcgtc ttcttcctgc tgctggtggt cgcgctctgg ccggttgccc ggcacgaccc
    10561 cgacccgctg acgcggtacc tggcgtgcgg cacgtggctg gcgctgctcg gctggaccat
    10621 cgggtcggtg gggccgagca gttcggtggg cttcgcgccg atgtggatcc tgtacgggct
    10681 ggggctcgcg gtggtgtcac gctcgcggct ggcgacgatg gagcgggaga cgagcttgga
    10741 gacgggcgga ccggctcgcc cgcccggcgc gccggcgagt atcatcgcgg aggcgccggg
    10801 cgccccggcg aaggcggcaa gcggcggagc cggccgcgcc gccgcaccct gaaaggacgt
    10861 catgcgcctc gacacgtttc tcaagcgcgc cgccgagcgc catccggcgc tcgtcctcca
    10921 ggtctcctgg gcgaacggcc tcgacatcat ccgcgacctc tcggccgagg gcgtgccgct
    10981 gctcgcgctc gacgccaacc cgcgcgccct cggcctgcgc tcacgcctcg ccgccggcag
    11041 cgtctgcccg gacccccagc gtgacgagga ggccttcgtc tcgttcctcg aggacctggg
    11101 ccggcgcctg ccccgcaagg gcgtcctgtt ccccacgcac gaccagtaca tctggcccgt
    11161 ctcgaagcac gccgagcggc tggagcagtg gttcctgatc ccgttctctc gctgggagac
    11221 catgcggcgg ctgcacgaca agcgcgccca gatggaggcg gcctggcgcg tgggcgtcga
    11281 caccccgaag acggtgttcg tggactccgc cgccgacctc gagcgcggcg tcgaggagat
    11341 cggctttccg tgcatcttca agccggtcga gtcgctcgcg ttcaagacgc gcttccgccg
    11401 gcacgtgctc gagatcggct ccgccgagga gctgcacgag gtctacgcca aggtcgacga
    11461 ctgcggcacg ctgatgctgc aggacatcgt gccgggcggg gacgaagagc tctacacgct
    11521 gggctcgtac ctcgacgcgc agagccggcc gctggcgcag ttcacgggac acaaactgcg
    11581 ccagcacccc ccgcggttcg gccacgtgag catggccgtg agcacgtggg tgcccgagct
    11641 cgcggaggcg ggactccgcc tgctccacga gctcgggtac cacggcgtca gccaggtcga
    11701 gttcaagcgc gatccgcgcg acggccgctt ccgcctcatg gaggtcaacg cgcgccactg
    11761 gatgtggcac tcgctcgccg ccgcctgcgg cgtcaacctc tcgctcgccg cctaccgcga
    11821 cgccatcggt gacccgtaca tcaccgcccg ccaggtcgac ggtctccggt gggtcgtctc
    11881 gctcaccgac gcgcgcgacg cgttctctcg ctggcgccgc ggcgagga
//
LOCUS       DASUMX010000142         3126 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1048978, whole genome shotgun sequence.
ACCESSION   DASUMX010000142 DASUMX010000000
VERSION     DASUMX010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3126
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1048978"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..1615
                     /locus_tag="VFZ86_06860"
     CDS             68..1615
                     /locus_tag="VFZ86_06860"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX5642047.1"
                     /translation="MPVNGAATTGKSLAIVGGGIAGLCAGCYAQMNGYRSRIYEMHDL
                     PGGLMTAWERKGYTVDYCIQWLVGSSPETGMHKLWREVGLLRDTRIVDLDIWVEYEAA
                     DGRRVTFWRDLDRLEAHLCELSPADADLVKRLLKDARRLATVDMPADLPPRELMRLRD
                     TLRTGPRMLPWMLPARRWGKLTVGELTDRFHSVLLRDAIRSLMPSPIGAMALLGTWAW
                     LHAGVAGYPLGGSLPLARNLERRYAELGGEIRYEARVARILTQQTGGADRAAGVELAD
                     GTQEPAEIVISAADGHATLYEMLGGAYLDDELRGVYEREELPLFSPILFVGVGVAREF
                     ADEAQRISGLLFPVDEPIGAGAVHSGRLEARILNFDPAMAPEGKTVITAILEADGPYW
                     LELREQDRQRYNAEKARIGEAVVRALDRRYPGLKDQVEMVDVATPATTVRYTGNWRAS
                     FEGWMPTPGYLMKGLPRRLPGLDDFYMVGQWVQPGGGLPTGVMTAREVLQLVCRRDGV
                     AFRTTAV"
     gene            1620..2309
                     /locus_tag="VFZ86_06865"
     CDS             1620..2309
                     /locus_tag="VFZ86_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006768880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642048.1"
                     /translation="METSTELESAYRFGFWIAIAVAVLTAATFALALTALPDKVPYPF
                     TDPVIAEQWPGDYLWMYPAMVLMLLFVALVAAIHVCAPPARRVFSLIALSVAILAAAV
                     LLADYYLQASVMQTSLEKGQLDGWSLLTQYNPNGVFIALEELGYLLMGVVFLCLAPVF
                     IGTSGVERALRWLLGASFAASVLALVVVSAAKGTDRGDTFEIAVISIVWLTLIAAGVL
                     TAIVFRRAGRA"
     gene            complement(2316..>3126)
                     /locus_tag="VFZ86_06870"
     CDS             complement(2316..>3126)
                     /locus_tag="VFZ86_06870"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEX5642049.1"
                     /translation="RLSFGLRRPRLGIRGTDMAGIVEAVGKNVTQLRVGDEVFGRGEG
                     TFAEYAAAPEKHLVTKPAGVTFEQAAAVPMSGLTALQALRDAGGIQPGQKVLVVGAGG
                     GVGSFAVQIAKSFGAEVTGVCSTSKLGYVRSIGADHVIDYTREDFTAGEKRYDLVLDN
                     VLQHSLTELLRALERNGTLVPNGGQFHKRWFASTGVLLIRAPLLSLKVPQRIRVCHER
                     PGRDDLLVLKELMESGRLTPIVGSTYPLSRTHEAISSFGEGHTKGKIVITL"
BASE COUNT          429 a         1126 c         1094 g          477 t
ORIGIN      
        1 agcagagtcg ccgcagcgag ccggccacgc gctcgcggcc tcgacccgcc gaccgccgga
       61 ggtcctcatg ccagtcaacg gagcagctac cacgggcaag tccctggcga tcgtcggcgg
      121 cggcatcgcc ggcctgtgcg ccggctgcta cgcgcagatg aacggctacc ggtcgcgcat
      181 ctacgagatg cacgacctcc ccggcgggct gatgaccgcc tgggagcgca agggctacac
      241 ggtcgactac tgcatccagt ggctggtcgg ctcgtctccc gagaccggca tgcacaagct
      301 gtggcgcgag gtcggcctgc tgcgcgacac gcgcatcgtc gacctggaca tctgggtgga
      361 gtacgaagcc gccgacggcc gccgggtgac gttctggcgc gacctggacc gtctcgaggc
      421 gcacctctgc gagctgtcgc ccgccgacgc cgacctcgtc aagcgcctgc tgaaggacgc
      481 gcggcggctc gccaccgtcg acatgccggc ggacctgccg ccgcgcgagc tgatgcgcct
      541 ccgcgacacc ctccgcaccg ggccccgcat gctcccgtgg atgctgcccg cccggcgctg
      601 gggcaagctc accgtcggcg agctcaccga ccggttccac tccgtgctcc tgcgcgacgc
      661 tatccggtcg ctgatgccct cgccgatcgg cgccatggcg ctgctcggca cctgggcctg
      721 gctgcatgcg ggcgtggccg gctaccccct cggcggctcg ctgcccctgg cccgcaacct
      781 cgagcgacgc tatgcggagc tcggcggcga gatccgctac gaggcgcgcg tcgccaggat
      841 cctcacccag cagaccggcg gcgccgaccg cgccgccggc gtcgagctgg ccgacggcac
      901 ccaggagccg gccgagatcg tgatctcggc ggccgacggc cacgcgaccc tctacgagat
      961 gctgggcggc gcctacctcg acgacgagct gcgcggcgtc tacgagcgcg aggagctgcc
     1021 gctcttctct ccgatcctct tcgtcggcgt cggcgtcgcc cgcgagttcg cggacgaagc
     1081 gcagaggatc tccgggctgc tcttcccggt cgacgagccg atcggcgccg gcgccgtgca
     1141 cagcgggcgg ctcgaggcgc gcatcctcaa cttcgacccg gccatggcgc ccgagggcaa
     1201 gacggtcatc acggcgatac tggaggcgga cggcccgtac tggctcgagc tgagggagca
     1261 ggacaggcag cggtacaacg ccgagaaggc gcgcatcggc gaagccgtcg tgcgcgccct
     1321 cgaccgccgc tacccggggc tgaaggacca ggtggagatg gtggacgtcg ccacgccggc
     1381 caccacggtc cgctacaccg gcaactggcg cgccagcttc gagggctgga tgcccacgcc
     1441 cgggtacctc atgaagggcc tgccccggcg cctgcccggg ctcgacgact tctacatggt
     1501 ggggcagtgg gtgcagcccg gcggcgggct gccgaccggc gtcatgacgg cacgggaggt
     1561 gctgcagctc gtctgcaggc gtgacggggt ggccttccgg acgacggcgg tctgaggcgg
     1621 tggagaccag cacggagctt gagagcgcgt accggttcgg gttctggatc gcgatcgccg
     1681 tcgccgtcct gacggctgcg accttcgccc tggcgctgac ggcgctgccc gacaaggtcc
     1741 cgtacccctt caccgacccg gtgatcgccg agcagtggcc gggagactac ctctggatgt
     1801 atccggccat ggtgctcatg ctcctcttcg tcgcgctcgt ggccgccatc cacgtgtgcg
     1861 ctcctcctgc gcggcgggtc ttcagcctca tcgcactcag cgtcgcgatc ctcgccgcgg
     1921 cggtcctgct cgccgactac tacctgcagg cgagcgtgat gcagacgagc ctcgagaagg
     1981 gccagctgga cggctggtcg ctgctcacgc agtacaaccc caacggcgtc ttcatcgcgc
     2041 tcgaggagct cggctacctg ctcatgggcg tcgtgttcct gtgcctcgcg ccggtgttca
     2101 tcgggacctc cggcgtggag cgggccctgc gctggctgct cggggcgagc ttcgccgcgt
     2161 cggtgctcgc cctggtcgtg gtctcggcag cgaagggcac ggaccgcggc gacaccttcg
     2221 agatcgccgt catctccatc gtctggctga cgctcatcgc cgcgggagtg ctgaccgcga
     2281 tcgtgttcag gcgcgcgggg agggcgtagc ctgtctcaca aggtgatgac gatctttcct
     2341 ttggtgtggc cctccccgaa ggacgagatg gcctcgtgag tccggctcag ggggtacgtg
     2401 ctgcccacga tgggcgtcag ccggccggac tccatgagct ccttcaagac gagcaggtcg
     2461 tcccgtcccg ggcgctcgtg acacacgcgg atccgctgcg gcaccttcag cgagagcagc
     2521 ggcgccctga tgagcaggac ccccgtgctg gcgaaccagc gcttgtggaa ctggccgccg
     2581 ttgggcacga gcgtgccgtt gcgctccagc gctcgcagga gctcggtcaa tgagtgctgc
     2641 agcacgttgt cgagcacgag gtcgtagcgc ttctccccgg cggtgaagtc ctcccgggtg
     2701 tagtcgatga cgtggtcggc gccgatcgag cggacgtaac cgagcttcga ggtgctgcac
     2761 acgccggtca cctcggcgcc gaacgacttg gcgatctgca ccgcgaagct gccgacccct
     2821 cctccggcgc cgacgaccag caccttctgc cccggctgga tgccgcccgc gtcgcgcagc
     2881 gcctggaggg cggtgaggcc ggacatgggg acggccgccg cctgctcgaa cgtgacgccg
     2941 gccggcttgg tgacgaggtg cttctcgggc gcggccgcgt actcggcgaa cgtgccctcg
     3001 ccgcggccga agacctcatc tccgacccgg agttgcgtca cgttcttgcc gacggcctcg
     3061 acgatcccgg ccatgtccgt gccgcggatg ccgagcctcg gccggcgcag cccgaaggac
     3121 aggcgg
//
LOCUS       DASUMX010000143         6850 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1913199, whole genome shotgun sequence.
ACCESSION   DASUMX010000143 DASUMX010000000
VERSION     DASUMX010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 6850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6850
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1913199"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            210..767
                     /locus_tag="VFZ86_06875"
     CDS             210..767
                     /locus_tag="VFZ86_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642050.1"
                     /translation="MTKRLKISSAMTAVLAALALAVVLAGCGDASPSETPSTIYPESS
                     ATGTLPALKAYLDDAQDVLAQVSTTVGALPDAVGEMSRKPDDTWSASAAQLQSIATQL
                     GDEAAALAALEPPSGLQPVQDAVVKGIEAAQKGVEKLATQLDSRVESAATRKDQVQAK
                     AGEIKAQLDGLAQQLKGALGGLTGQ"
     gene            764..1678
                     /locus_tag="VFZ86_06880"
     CDS             764..1678
                     /locus_tag="VFZ86_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019810376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5642051.1"
                     /translation="MTSAVVTERLTKSYGRRRSRGVVDLDLEVRTGEVFGFLGPNGAG
                     KTTTIRVLLDLIRPTAGRVLVLGRDSRRETLAIQARSGYLPGELSLYPNLTGRETLRY
                     LASLRGGVDWDYVAALTERLDCDLERKVADLSTGNRRKIGLIQAFMHRPELLILDEPT
                     SGLDPLVQHEFYHLLDEVRDAGQTVFLSSHVLPEVQRVCDRVAFVREGRLVAVEDVAE
                     LMGRAVREIEVVFAEPVPPSSFENVPGVTRVLADGKDATTLRLTVTGSLDPAVKALAA
                     HPVVDLISQLPDLEDVFMTFYEGGDDAA"
     gene            1668..2468
                     /locus_tag="VFZ86_06885"
     CDS             1668..2468
                     /locus_tag="VFZ86_06885"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX5642052.1"
                     /translation="MLRNAFVKGLRDQLRPFVMWAAGVAFYVALLISIYPSIRKSAGA
                     INEYVKNMPEAVRVAFMGSSDFTTPVGYVNVELLSWLAPIVLIAFAVAVAARSLAGEE
                     ENGTLGLVLTHAVGRRRFALQKYAAMTVVVIALGATFWLSLTVATAIAGTPVGAGDLA
                     VAFVRLTLLGLAVGSVTFAVGGATGRRQAGIAAGAGVGVVMYLLNTLAVMNETIRPLR
                     HVSLFHWAGGASPLGKPLDATGVLVLVATSAVLLVATLVLFERRDVRV"
     gene            complement(2759..2959)
                     /locus_tag="VFZ86_06890"
     CDS             complement(2759..2959)
                     /locus_tag="VFZ86_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642053.1"
                     /translation="MSVEAVHDYICSVCKEWFSVTGPADGDGLVCPACGSGRVREHWE
                     SRVRNAAKNPPRPIEEMRDCVG"
     gene            complement(2956..3336)
                     /locus_tag="VFZ86_06895"
     CDS             complement(2956..3336)
                     /locus_tag="VFZ86_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020404366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5642054.1"
                     /translation="MASAITWFQIPATDSARAKAFYETICGFELLPLDAGPGMEMWGF
                     PSDWQQGEVGGAIVTGEGAVPSATGTAVFLNANPDLQVMLDRVEGAGGTILMPKTEIG
                     MEGAGHFAMITDTEGNTLGLHSQG"
     gene            complement(3383..3907)
                     /locus_tag="VFZ86_06900"
     CDS             complement(3383..3907)
                     /locus_tag="VFZ86_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642055.1"
                     /translation="MDAPTSRRAAGWRRLTPRTGVRAQLLAAAVVWLVGTAILLVRGV
                     QYIVAPEEYERFGYGIALIALAAIAIGIVKARFILIRYACKAVTRIRTRGHACFFGLF
                     AWSSWLFILVMMGGGVLLRHSPLIDSWWGRTFLGILYVAVGTALAIADVVFWRAALGP
                     VETARDVSSPPGRQ"
     gene            complement(3928..4326)
                     /locus_tag="VFZ86_06905"
     CDS             complement(3928..4326)
                     /locus_tag="VFZ86_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009079712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX5642056.1"
                     /translation="MVLDTSVIVAILLGQAEAAALHQLLVVQSPSLVGSPTLAEAGIV
                     LQGRIGQTGLAALRGFLVEYDVHAVPFGEEHWRLAAQAFGRFGKGRHPAGLNFGDCLT
                     YATAKLAARPLLCVGDDFAQTDLELVPPPS"
     gene            complement(4330..4608)
                     /locus_tag="VFZ86_06910"
     CDS             complement(4330..4608)
                     /locus_tag="VFZ86_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014630035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HEX5642057.1"
                     /translation="MALTIKNPEVERLAAEVARLTGETKTQAIRVALEERRRRLSSDV
                     DQEARAAEILHWLETEVWSLVPEELRGQPHDSRRDDEIIGYGPDGLPE"
     gene            complement(4706..4990)
                     /locus_tag="VFZ86_06915"
     CDS             complement(4706..4990)
                     /locus_tag="VFZ86_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642058.1"
                     /translation="MLIIGAAPVFMWLVPGHKGWTVILGGLAALAALVAGILLAGRWL
                     GVFQRRRENPLEQQQGCLQLLVTLLALLAGAAILYYVIYYGAGAYLRGEL"
     gene            5202..5957
                     /locus_tag="VFZ86_06920"
     CDS             5202..5957
                     /locus_tag="VFZ86_06920"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX5642059.1"
                     /translation="MGKSRKRKPNRKSDPPAGAAGNDAPGKSVPASGAPRSTSPAGSG
                     FFVRAAPWAGATALVVLAVAVLYFFTGAAATEAFEAPPVAADDLSYGPENPELTVIEY
                     SDFQCPFCAEYAKWMMQLRAKYGDRVKFVFRNYPLADHEWAQFAAQVAYAASRQGKFW
                     EMHDLLFERQDEWAASSDPRPIFDSYAESLGLDLDQFHADADAQATLDFIKQQATAGK
                     EAGVSHTPWFVIGDESVLPRSLEEFDKTIQERL"
     gene            5957..6475
                     /locus_tag="VFZ86_06925"
     CDS             5957..6475
                     /locus_tag="VFZ86_06925"
                     /inference="COORDINATES: protein motif:HMM:NF019499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HEX5642060.1"
                     /translation="MGAPDPAAAAARPARAAPATRRWLPWTLAALAYAGFVISVYLTI
                     VHYRGYVSPCYVVQGCETVQTSEYSVILGVPVALAGAVFFGLLFYLTVGLLRSSAPTL
                     VLVFKVLAFLGALAIIPLFLLQAIVLKAFCSYCLATEVLMLAIWLLSFLLTAPGRSGE
                     PLEASAGATTVE"
     gene            6472..>6850
                     /locus_tag="VFZ86_06930"
     CDS             6472..>6850
                     /locus_tag="VFZ86_06930"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEX5642061.1"
                     /translation="MTRHAATLVAAAVLVVSLAAAAVPARAHAAPAKGLVDASLEALP
                     AGSAAAPVMIREIDQRLGARWIRLTISWAVLEPQQGAYSPIELERLDALVQDLHTAGV
                     KIILTVCAAPAWAQDSSLWQNPPV"
BASE COUNT         1032 a         2471 c         2361 g          986 t
ORIGIN      
        1 ccagcccgtg ccgaccacgg cgacgacgca cgcccacgcc agcagcacga tccacgcgcg
       61 ccgcacgcac cagtcgccga gtctatagag gagggtggac acgcgaggga gtatccttcc
      121 tgtggccggg attgacgata cgtaccccgg cccgggtgtc aaccgcagta cgcaacgccg
      181 cccccgcggg ggacggcaag ggaggcaatg tgacgaagag gctcaagatc agcagcgcga
      241 tgacggcggt gctcgccgcg ctcgccctcg ccgtggtcct cgccggctgc ggggacgcca
      301 gcccgagcga gaccccgtcg accatctacc cggagtcgtc ggccaccggc acgctccctg
      361 ctctcaaggc gtacctcgac gacgcccagg acgtcctcgc ccaggtgagc accacggtcg
      421 gggcgctccc ggacgctgtc ggcgagatga gcagaaagcc cgacgacacg tggtcggcgt
      481 ccgcagcgca gctgcagagc atcgccacgc agctcggcga cgaggcggcg gcgctcgccg
      541 cactcgagcc gcccagtggc ctgcagccgg tccaggacgc cgtcgtcaag ggcatcgagg
      601 cggcgcagaa gggcgtcgag aagctggcga cgcagctcga ctccagagtg gagagcgcgg
      661 cgacgaggaa ggaccaggtg caggccaagg ccggcgagat caaggcgcag ctcgacgggc
      721 tcgcgcagca gctgaagggc gcgctcgggg gcctcacggg ccaatgacct cggccgtcgt
      781 caccgagcgc ctgaccaaga gctacggcag gcggcgcagc cgcggcgtcg tcgatctgga
      841 cctggaggtc cggaccggcg aggtgttcgg cttcctcggg cccaacggcg ccggcaagac
      901 gaccaccatc cgcgtgctgc tggacctgat ccggccgacg gcggggcgcg tcctcgtcct
      961 cggacgggac agccgccgcg agacgctggc gatccaggcg cgcagcggct acctgcccgg
     1021 cgagctgagc ctctacccca acctcaccgg gcgggagacg ctgcgctacc tcgccagcct
     1081 gcgcggcggc gtggactggg actacgtcgc cgcgctcacg gagcgcctgg actgcgacct
     1141 cgagcgcaag gtcgccgacc tctccaccgg caaccggcgc aagatcggcc tcatccaggc
     1201 gttcatgcac cggcccgagc tgctgatcct cgacgagccc acctcgggcc tcgaccctct
     1261 cgtccagcac gagttctacc acctgctgga cgaggtccgc gacgccggcc agacggtgtt
     1321 cctctcctcg cacgtgctgc ccgaggtgca gcgcgtgtgc gaccgcgtcg ccttcgtgcg
     1381 tgagggccgg ctcgtcgccg tggaggacgt cgccgagctc atgggacggg ccgtgcgcga
     1441 gatcgaggtc gtgttcgcgg aaccggtccc gccgtcctcg ttcgagaacg tacccggcgt
     1501 cacgcgggtg ctggcggacg gcaaggacgc gaccacgctc cgtctcaccg tgaccggctc
     1561 cctggacccg gccgtcaagg cgctcgcggc tcatcccgtc gtcgacctca tcagccagct
     1621 gcccgacctc gaggacgtct tcatgacgtt ctacgaggga ggcgacgatg ctgcgtaacg
     1681 ccttcgtcaa gggcctgcgc gaccagctgc gcccgttcgt catgtgggcc gccggcgtgg
     1741 ccttctacgt ggcgctgctc atctccatct acccgagcat caggaagagc gccggcgcca
     1801 tcaacgagta cgtcaagaac atgccggagg ccgtcagggt ggcgttcatg gggtccagcg
     1861 atttcacgac tccggtgggg tacgtgaacg tcgaactgct gtcgtggctc gcgccgatcg
     1921 tcctcatcgc gttcgcggtc gcggtcgccg cccggtcgct ggccggagag gaggagaacg
     1981 ggacgctcgg tctcgtcctc acgcacgccg tgggacgccg gcggttcgcg ctgcagaagt
     2041 acgccgccat gaccgtggtg gtgatcgcgc tgggcgcgac gttctggctg tcgctgaccg
     2101 tcgcgacggc gatcgccggc acgccggtcg gcgcaggcga cctcgccgtg gccttcgtgc
     2161 ggctgacgct gctcggcctg gccgtgggca gcgtcacctt cgccgtgggc ggcgcgaccg
     2221 ggcgccggca ggccgggatc gcggccggcg ccggcgtggg cgtcgtcatg tacctgctga
     2281 acacgctcgc ggtgatgaac gagacgatcc gccccttgcg ccacgtgtcg ctgttccact
     2341 gggcgggagg cgcctcgccg ctcggcaagc ccctggacgc gaccggcgtg ctcgtgctgg
     2401 tcgccacctc ggcggtgctg ctggtggcga ccctggtgct gttcgagcgg cgcgacgtgc
     2461 gcgtctgacg ccggcctcga gcgacgtcgg cgagtccagc ccggcgcgcg ccgcgtcgcg
     2521 ggagacgcgt cgctgagtcg cgtggcgcga aggtatgccc tcaccagagt gacacggccg
     2581 cacgtacgct tgcccgtacg cttgtacaac tggaagcacc tttgatggtg ccagtccccg
     2641 aagagactac ctgcttcccg ggccggggtg gcggcgaggc ggataggcct ctggtcgggc
     2701 ccgctcctcg gccgttctcg cggcgcgacc gcgccgccgg gggtcagccc ccgccgcctc
     2761 agccgacgca gtcgcgcatc tcctcgatcg gcctcggcgg attcttggcg gcgttacgga
     2821 cgcggctctc ccagtgctcc cgcacccggc cggagccgca cgccgggcac accagcccgt
     2881 cgccatccgc cgggccggtc acggagaacc actccttgca gacgctgcag atgtagtcgt
     2941 gcacggcctc cacgctcaac cctgcgagtg cagcccgagg gtgttgccct cggtgtcggt
     3001 gatcatggcg aagtgcccgg cgccctccat gccgatctcc gtcttgggca tgaggatcgt
     3061 gccgccggcg ccctcgactc tgtcgagcat cacctgcaga tccgggttcg cgttcaagaa
     3121 gaccgcggtg ccggtcgcgc tgggaacggc tccctctccg gtcacgatcg caccgcccac
     3181 ctccccctgc tgccaatcgg atgggaaccc ccacatctcc atgcctgggc cggcgtccag
     3241 cgggaggagc tcgaacccgc agatcgtctc gtagaacgcc ttcgcgcgcg cactgtcggt
     3301 tgccgggatc tggaaccacg tgatggcgga tgccatgagg agcctcctcg gtgacttctc
     3361 cccttgcggg gccttgccac tctcattgcc tcccgggcgg tgaagaaacg tctcgggccg
     3421 tctcgaccgg gcccagcgcg gcgcgccaga agaccacgtc ggcgatcgcg agcgccgtgc
     3481 cgacggcgac gtagaggatg ccgaggaacg tgcgtcccca ccaggaatcg atgagcggcg
     3541 agtgacgcag cagcaccccg ccgcccatca tgaccaggat gaacagccac gagctccacg
     3601 cgaacaggcc gaagaagcag gcgtgcccgc gcgtgcggat ccgcgtgacc gccttgcacg
     3661 cgtagcggat gaggatgaaa cgcgccttga cgatcccgat ggcgatcgcc gccagggcga
     3721 tgagcgcgat cccgtagccg aagcgctcgt actcctccgg ggcgacgatg tactgcacgc
     3781 cgcggacgag caggatggcg gtgccgacga gccagaccac cgccgcggcg aggagctggg
     3841 cgcgcacgcc ggtgcgcggc gtgagccggc gccaccctgc tgcgcgcctc gaggtcggag
     3901 catccatggg ctcgaggata gcaggagcta ggagggaggc gggaccagct ccagatccgt
     3961 ctgggcgaag tcgtccccta cgcagagcag cggcctagcg gcgagcttgg cggtggcata
     4021 ggtgagacag tcgccgaaat tgaggccggc gggatggcgg cccttgccaa acctgccgaa
     4081 cgcctgcgca gccagccgcc agtgctcctc tccgaacgga acggcgtgca cgtcgtactc
     4141 gaccaagaag ccgcggagcg cggcgagccc cgtctgcccg atcctcccct gcaggacgat
     4201 gccggcctcg gcaagcgtgg gcgaacccac cagactgggc gactgcacga ccagcagctg
     4261 atggagcgcc gcggcctcgg cctgccccag gaggatcgcg acgatcaccg acgtgtcgag
     4321 gaccacgggc tactccggga ggccgtcggg cccgtagccg atgatctcgt cgtcgcgacg
     4381 cgaatcgtgc ggctggccgc ggagctcctc cggcaccagc gaccagacct cagtctccag
     4441 ccagtggagg atctcggcgg cccgcgcctc ctggtcaacg tcgctgctga gccgccgccg
     4501 acgctcctcg agcgcgacgc ggatggcctg cgtcttggtc tcgccggtga ggcgtgcgac
     4561 ctctgcggcg agccgttcga cctcggggtt cttgattgtc agggccacag tcatctcccc
     4621 agaatcatgg cgatgcctgc atggtacctt caaggttgac agcagtaaac gaagtggacg
     4681 ttcgacgtcg agacgacgac ggagctcaga gctcgccgcg caggtaggcg ccggcgccgt
     4741 agtagatgac gtagtagagg atggccgcgc cggcgagcag cgccagcagg gtcaccagaa
     4801 gctgcaggca gccctgctgc tgctcgaggg gattctcgcg gcggcgctgg aacacgccca
     4861 gccatcgacc ggcgagcagg atgcccgcca ccagcgccgc gagcgcggcg agaccgccca
     4921 ggatgacggt ccagcccttg tggcccggta ccagccacat gaagaccggc gcggcgccga
     4981 tgatcagcac cagccccgcg atcgtgcgtc caacgccaac gcgcgtccgt tccccctctg
     5041 tcatggacct cccccgtcgc gctccgaccc cacgccgcag cgtgacccgc ccagcatacc
     5101 cccgtgcgga cgtgcctcgc cgaggcgatg gtatcgagcg atcacacgcg ccgaacgggg
     5161 gtagaatcgg ccccagtctc gaacccggcg cggaaggtct cgtgggcaag tccagaaaga
     5221 ggaagccgaa ccggaagagc gacccccccg ccggcgcagc cggcaacgac gcgccgggca
     5281 agagcgtgcc cgcctccggt gcaccgcgga gcacgagccc cgccggcagc ggcttcttcg
     5341 tcagggcggc gccctgggcg ggggccacgg cgctggtcgt cctggccgtg gccgtcctgt
     5401 acttcttcac cggcgccgcc gccacggagg ccttcgaggc gccgccggtc gccgccgacg
     5461 acctcagcta cggcccggag aaccccgagc tcaccgtcat cgagtactcc gacttccagt
     5521 gccccttctg cgccgagtac gccaagtgga tgatgcagct gcgcgccaag tacggcgaca
     5581 gggtcaagtt cgtcttccgt aactatccgc tggccgacca cgagtgggct cagttcgccg
     5641 ctcaggtcgc gtacgccgcc tcgcggcagg gcaagttctg ggagatgcac gacctgctct
     5701 tcgagcggca ggacgagtgg gccgcgtcca gcgacccgcg gcccatcttc gactcctacg
     5761 ccgagagcct cggcctcgac ctggaccagt tccacgccga cgccgacgca caggccacgc
     5821 tcgacttcat caagcaacag gccaccgcgg gcaaggaggc cggcgtgagc cacacgccgt
     5881 ggttcgtgat cggcgacgag tcggtgctgc cgcgcagcct ggaggagttc gacaagacca
     5941 tccaggagcg gctctgatgg gcgcgccgga tccggctgcc gccgcggcgc gcccggcgcg
     6001 agccgcgccc gccacgcgcc gctggctccc gtggacgctg gcggcgctcg cctacgcggg
     6061 tttcgtcatc tccgtctacc tgaccatcgt ccactaccgc ggctacgtct cgccctgcta
     6121 cgtggtgcag gggtgcgaga ccgtgcagac cagcgagtat tccgtgatcc tcggggtgcc
     6181 ggtggccctg gcgggggccg tgttcttcgg cctgctgttc tacctgaccg tgggcctgct
     6241 gcggagttcc gcgcccacgc tcgtgctggt gttcaaggtg ctggcgttcc tgggcgcgct
     6301 ggcgatcatc ccgctgttcc tgctgcaggc gatcgtgctc aaggcgttct gctcgtactg
     6361 cctcgccacc gaggtgctca tgctcgccat ctggctgctg agcttcctgc tgacggcgcc
     6421 gggccggagc ggcgagccgc tcgaggccag cgccggcgcc accaccgtcg aatgaccagg
     6481 cacgctgcaa cgctggtagc ggcggccgtg ctcgtcgtct ccctcgccgc cgcggctgtg
     6541 cccgcgcgcg cgcatgccgc gccggcgaag ggcctggtcg acgcctctct cgaggcgctg
     6601 cccgcgggct cagcggctgc accggtcatg atccgcgaga tcgaccagcg gctcggcgcg
     6661 cggtggatcc gcctgaccat ctcgtgggcg gtgctcgagc cacagcaggg ggcgtactcc
     6721 cccatcgagc tcgagcgcct cgacgcactg gtgcaggacc tgcacacagc gggcgtcaag
     6781 atcatcctca cggtgtgcgc ggcgcccgcc tgggcgcaag actcctcact ctggcagaac
     6841 ccgcccgttg
//
LOCUS       DASUMX010000144        17692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_619804, whole genome shotgun sequence.
ACCESSION   DASUMX010000144 DASUMX010000000
VERSION     DASUMX010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 17692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17692
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_619804"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="VFZ86_06935"
     CDS             <1..200
                     /locus_tag="VFZ86_06935"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX5642062.1"
                     /translation="GEVDAFWELDLSPWDRAAGALIAREAGAAVELLPAAHGSGPAVV
                     AAHPSLLPSLLELLREAGALA"
     gene            complement(250..942)
                     /locus_tag="VFZ86_06940"
     CDS             complement(250..942)
                     /locus_tag="VFZ86_06940"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX5642063.1"
                     /translation="MAGGGEGAGDSGARGFDGVIFDLDGTLVDTLEDIADAMDRVLAL
                     EGAPGHSYDEYRYLIGHGIRNLVTESLPAELRSDERVARCYERMIEDYGRNSLNKTRV
                     YDGVPELVRELRSAGVPLAIHSNKADAPTQAIVAALLDPADFVHVAGARPDAPLKPDP
                     AVALAIAARFGLPPGRVAYLGDSLVDMRTATAAGMIAVGAAWGFRTPEELMQSGAAAV
                     IAAPLDLLELRG"
     gene            complement(951..1403)
                     /locus_tag="VFZ86_06945"
     CDS             complement(951..1403)
                     /locus_tag="VFZ86_06945"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525309.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the K subunit is a
                     nonenzymatic component which binds the dimeric form by
                     interacting with the G and E subunits; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HEX5642064.1"
                     /translation="MSPEAAAEMGRIGAVAAMALAATGSALGTYLGGAGAVGAWKKLT
                     AQGKPAPFTLITFVGAPLSQTIYGMILMITMASKADAGYDPWALLGVGIVAGLGIGMS
                     AYGQGKVGASCADALAETGQGFSNYIAVIGIVETVAIFVLVFAILALG"
     gene            complement(1441..3273)
                     /locus_tag="VFZ86_06950"
     CDS             complement(1441..3273)
                     /locus_tag="VFZ86_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642065.1"
                     /translation="MIVRMKRLTLLCLLHDRDAALDELRGLGVVHVTDRRPPAGEELE
                     ALRARLAGAERALAALPEKPARGVTATAPVDAATTRADAATLPAEDAVAEVLALLDRG
                     DALAVRAGELRRELARYAGFGEFDLDEVRELQRAGVWTALAGAPPDERLTAPDGVVLT
                     ELGRDRGGRRLVLAVLGGRPDPLPDLGGHYELVPWPERSLAELRDEVAAAEAETDAAA
                     RRLTELAAARAAVAALARATADQVRWVEVRAGMDEAGELSVLSGYVPAAEQDAIREAA
                     AAHGWGILLDDPQPGEDVPVQLHQSRWVRPISTVFDFLHIYPGYWEADAGWVILPFFS
                     LFFAMIVGDAGYAVLLLVLTAVLQWRLKKVPAHVFHMMYIVGFATLVWGVLSGSYFGI
                     PTLPRFAEQLQVAWLADRDNLIDLCFLIGAVHLSFAHVWNAVSFIREGAWTKVVAQGG
                     WLLVVWSMFFIARSAVLGRGAPGFLLLMLLTGVVVVAAFMKTPAELKASWIDHAMLPL
                     TLVGNFVDILSYIRLFAVGYASVAVLAAFNDMAASIGFDSPLTAVAASVLLLFANALN
                     IVLAGLGVLVHAVRLNTLEFSTHKGLAWQGHTLYTPFAGPRGPA"
     gene            complement(3270..3884)
                     /locus_tag="VFZ86_06955"
     CDS             complement(3270..3884)
                     /locus_tag="VFZ86_06955"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020885596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HEX5642066.1"
                     /translation="MAKLKKTKNELKAQREALQRYQRFLPTLELKKQQLQREVRRAEA
                     ELRRREERAGELLAGLRPWAGLLTGPPDLTEYVRVEGLEVETVNVAGVNVPVLSGLRF
                     VPPAIDYFSTPAWLDDAVEVLGELVRLRAEIHVLSRQRELLGEELRLTSQRVNLFEKV
                     MIPRTLDNIRVIRIALGDAQTAAVARAKLAKSKTVSVLSGKSSA"
     gene            complement(3888..5201)
                     /locus_tag="VFZ86_06960"
     CDS             complement(3888..5201)
                     /locus_tag="VFZ86_06960"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HEX5642067.1"
                     /translation="MRRVYQQISQIAGNVITVQATGVGNQELASVEAPGFSSQAQVIR
                     VEGDYVTMQVFSGTRGVPTDAEVRFVGHGAQTPFSDALLGRVFTGGGVPRDRGPGLDD
                     DLIPIVGPPVNPARRVIPRTMIRTGIPMIDVFNSLVESQKLPIFSVAGEPYNELLARI
                     ALNAEVDLIVLGGMGLKYDDYLYFRDTLEDAGALSRAVLYVHTAADPVVECLLVPDTC
                     LAVAEKFALAGKRVLVLLTDMTNFADALKEVAVTMEQIPSTRGYPGDLYSQLAARYEK
                     AVDFEGAGSVTVLAVTTMPGDDVTHPIPDNTGYITEGQFYLRNGRIEPFGSLSRLKQQ
                     VNARTRDDHRTIMNTMIQLYAGFKESQEKQSMGFRMSAWDEKLLKYGGLFETRLMDLS
                     VTIPLEEALDLGWRILAECFAPQETGIPTALVERFWPAAASGVST"
     gene            complement(5194..6927)
                     /locus_tag="VFZ86_06965"
     CDS             complement(5194..6927)
                     /locus_tag="VFZ86_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020885598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HEX5642068.1"
                     /translation="MSLRTGRVMGVSGNLVTVEFDAAVSQNEVAYVLLGDLRLIAEVI
                     RVRGRYADMQVFESTAGIKVGDAVEFVGEMLSVELGPGLLSRVFDGLQNPLNVIAERH
                     GFFLPRGEQTDALPRDQVWEFAPRAAAGDRVRAGDTLGVVAEGIFEHRVLVPFGVTGV
                     ATVAEVAAAGEYTITDRIAVLRTADDEALEVAMLQRWPVKRPIRCYAERLRPTEQLVT
                     KMRILDTFFPVAKGGTYCVPGPFGAGKTVLQQLVSRHADVDVVVIAACGERAGEVVET
                     LREFPVLTDPRTGRSLMERTVIICNTSSMPVAAREASVYTAVTIAEYYRQMGLHALLL
                     ADSTSRWAQALREMSGRLEEIPGEEAFPAYLESVIAAFYERAGAVRLNDGTVGTVTIG
                     GTVSPAGGDFEEPVTQSTLKVVGAFHGLSRQRSDARRYPAIDPLDSWSKYGSIVPARE
                     LSLARSVLEKGHEVQEMMKVVEEEGTAMADFLVSLKAEYLDAVYLQQDAFDPVDGASS
                     AERQRHVFALIARILAADMDFADKDAARSYFQTLTQATRDWNRAAWDDDSFAAAETRV
                     AAMLSEVSPDA"
     gene            complement(6924..7460)
                     /locus_tag="VFZ86_06970"
     CDS             complement(6924..7460)
                     /locus_tag="VFZ86_06970"
                     /inference="COORDINATES: protein motif:HMM:NF022415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2764 family protein"
                     /protein_id="HEX5642069.1"
                     /translation="MEYVYLVASLPSLELTATPRISSSELLSSSAGVLREDHREDLRA
                     VLEDRPHDVRAPELRPYLDAETQLRTTVARLRAARAGAAYDAGTHPFAGYDARCASAA
                     ERAMELEDPRERELALDRLRWTLLDELAAAPPFGLQAVLAYGLKLRLAEKWAATDEAE
                     GLRVAAAVADAALAGSLP"
     gene            complement(7462..8085)
                     /locus_tag="VFZ86_06975"
     CDS             complement(7462..8085)
                     /locus_tag="VFZ86_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642070.1"
                     /translation="MTAELQSLLDRIRAEGVERADAEAARIVGEAEERAAGIVAGAER
                     QAAALRAAAETDAAAAVERGHTALEQAARDFLLSLQKSIESVLRETLCETVAGALTPD
                     VMAQILVRLADAYAAHDMNESRVDVLLSPEDRDAVGAIVMEKYRELVRQGLTLRPDER
                     VEDGFKVSFVDYNLYHDFRAEALAEALAPVLKPPLCDIVRRAAAEQG"
     gene            8346..10487
                     /locus_tag="VFZ86_06980"
     CDS             8346..10487
                     /locus_tag="VFZ86_06980"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5642071.1"
                     /translation="MARRRYQDEGGAIGFGGLFGTSFGYVGTSIYFVLGAIGLYALGV
                     TPLLILIVGLAFVATAWSYAEGSAAMPESSGVTSFTRRAFGPLAGFAAAWALLLDSIV
                     LVAIVCSFIPSYLSVFWPQVQDWPYDLLTSLTVLVVIVALNLLGAHASVRLNALMAFL
                     GVATLMLLVIVGFFVAVKPGLLWSQVAFGTAPTWRSLLYAIPLAVAAFIGLDAVSSRA
                     ETALRPSRDVPRAINLVLPLIVALAVGVAVVALSALPVGATVVPVDPSTGLTRPVAVV
                     PGDEPGVFVLAGDPATQVLVPVEQSGAGHVIPAQEPTGAVTASPDGPVTRLYGTLLGS
                     AYLQDPMMGLVAILPESLSGLEDVLRVWLAVLISVSLLLTANAVVGGSARVAFSLARH
                     HQVPAALGRVHATRMTPYVGIVLFGVAVVVLLVPNDPLLLLGLFGFGAALAFTAANAS
                     IVALRYKEPSISRPFAIPFSVRFRGVPLPVPAVVGAAAMAAVWVLMVGTHAAGAVVGF
                     AWLGAGLVLYAAYRRSTGYALLTQPREAAVPAAALSDVDYDRILVPVVGTRLSDEMMV
                     LGCQLAAEKDAVIDVLYVLEVPMNLPLDASLPDGRARGKHVLDTAIAVAREFGVEAWP
                     HLVSARSPGRAIVDTAREWDADVIIMGAVRRYRPDGKLVGDTVAHVMRQARAEVLLNL
                     VGEDYPLQGSAAEYDAEQAATKAASTSTTGA"
     gene            10495..10917
                     /locus_tag="VFZ86_06985"
     CDS             10495..10917
                     /locus_tag="VFZ86_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEX5642072.1"
                     /translation="MHVIIAGCGRVGSGMATDLVREGHDVVIIDEDPRSFALLGDEFP
                     GVRVVGQAIDWSVLRKAGIEGTDAFAAATDGDNTNVTCALIALRAFEVPCAVARVYDP
                     RRAEVFARLGIRTVCPTSGTTLMMLAAVLTCPSTPRTA"
     gene            10919..11638
                     /locus_tag="VFZ86_06990"
     CDS             10919..11638
                     /locus_tag="VFZ86_06990"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEX5642073.1"
                     /translation="MFAVIVGGGKVGLNVARSLTLLGHDFVVVEQRRTRFDLLRAELE
                     DHLLFGDGAELGTLEAAGIGVADLVVAVTGDDEDNVVIAMLARHEYGVPKVVARVNDP
                     RNQKTFDLLDVDATVCASSLVVSLIQHELPVHQFVPLLSLRREGLEIAEIAVSDDSPA
                     AHLPVGEIPLPPGVLIGAILRGGAAVHPGDGEVLRPGDQLLCLLPLQMERSLLSSLLP
                     ERRPEDVQAETYIDAGAGERA"
     gene            11663..13012
                     /gene="trkA"
                     /locus_tag="VFZ86_06995"
     CDS             11663..13012
                     /gene="trkA"
                     /locus_tag="VFZ86_06995"
                     /inference="COORDINATES: protein motif:HMM:NF007031.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEX5642074.1"
                     /translation="MRIIVVGTGDLGFHVAKRFSEEHHDVVVVGRDDEALHWAQEVLD
                     VSAVRGHGGSPSILVEAGVRSADIVVAATSNDETNIVACLIAEAIAPTATRVARLHDP
                     AYLGSSGIIDQSSLSIDVVISPEEEVAKAVAVLASVPGAADALEFAGGRVLAVGVEVD
                     EDSPMIGRTVAELRRKDKGGLLVAGVYRGEVVSAPTSDLRIQVRDVLFLVGGRDALRR
                     AMTRLGKTWARTRSAIIAGGGWEGVAIARQLAASGIHVKIIERDPAVCDEITRELGDA
                     VVLLGDAHDHSLLVDAGVQRSDLFVSALGSEADNIVVALLAKRLGCRRVVSLVDTPGQ
                     AAFASAIGVDSVVSPVLAALNTIVQLSRPGQVVTVRTLRESLVEGIEFVAMEGSGIVG
                     LQLALLRMPHGSLIGAIVRDGKAVIPESSTVVNAGDRVILFARPALLPRLRRLITAE"
     gene            13029..14528
                     /locus_tag="VFZ86_07000"
     CDS             13029..14528
                     /locus_tag="VFZ86_07000"
                     /inference="COORDINATES: protein motif:HMM:NF014443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEX5642075.1"
                     /translation="MTRRSSYAGRARVHPTTLSSARRVGYLLSLLLVFASLTLAVPLA
                     ISAGYREPETVGDFAATLLIGVVIGLAGYLFLRTDLAGLTRREGFAAVALGWLMVCAL
                     GSLPFALAGGLDPVNAWFETVSGFSGTGSSVIPDVESLPKGLLFWRSFTHWLGGMGFV
                     ALYIAIFPLMGVGAMQLFRAEAPGLEMDRLRPRIRSTARILWLIYLGMSVILTALLMA
                     GGMGWFDAVCHMFAAIGTGGFSTKNASVGWFDSAYIDWVIILFLWIASTNFALWWAVL
                     GGKPKRLLKDPEWRFYSLVLLGSSAVVSALVYLQNNVSLHDAIRNATFEVVSTASTTG
                     FATADYEKWAPLAQLILFLLLFMGGSAGSTAGAMKAVRVYLFAKQALRGLFTTIHPQS
                     VAPVRLGGRAVSPEVMRGISTFMGLYLLTYVVAVAAISITDIDFATAMSAVATAMGGV
                     GPGLGEIGPYDNFLWMHPFAKLVLSFCMLAGRLELFTLMLIFVPAYWRR"
     gene            14628..15386
                     /locus_tag="VFZ86_07005"
     CDS             14628..15386
                     /locus_tag="VFZ86_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002201599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5642076.1"
                     /translation="MCLRLEGYVTIVTGAASGIGKATAIRFAQEGAKVTCVDIDGDGV
                     KAVAEAIGGDAFGVQADISDPEQVKAYTDGTVERWGKLDVAFNNAGVNLPGVFHEVPD
                     EVIDRTLDVNVKGTMFGCRYAIPHMLEQGKGSLINTTSVNGIVAEPYLTVYAASKGAI
                     VMLSKGIALDYVKKGIRCNAIAPGWVDTPVNYAHAEMLGGLQHVYDTIDSFQPIGRPG
                     EPVEIANCALFLASDESSFVTGTVLVADGAMTAQ"
     gene            complement(15403..15693)
                     /locus_tag="VFZ86_07010"
     CDS             complement(15403..15693)
                     /locus_tag="VFZ86_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642077.1"
                     /translation="MRIRATDEVAAAVRERGGRLYVWTSAHRCCTGPLVLLETGSVPP
                     EGGAREFRAHDAGGFQVLFDPGERRPPDELVLELRGRRRKVVAFWDDQAWVG"
     gene            complement(15767..>17692)
                     /locus_tag="VFZ86_07015"
     CDS             complement(15767..>17692)
                     /locus_tag="VFZ86_07015"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX5642078.1"
                     /translation="ALIASLAVLLPLAAPTLTLILGQESIGAWPTTTTQRRAYDLIAE
                     GLGAGANGRLLIAARFSPVAEPSAAYTRKKARAERLAESLEQDAGRLKRQGRALERRG
                     DALKAEKAPLERRAAALTARKAALEAQAAPLLARRDALLAREQRLRAQKARLEAQGAA
                     LAAEGAALQQQGAALAAEGQQLQAQIAALQAQIAATTDPAELAELQTQLVTLAAQAQQ
                     VQQQIARVQADAAALQQEVAALEDRGAELAAEGRRLEREGAALQAESAALTAQGDALQ
                     AEGARLTKEGGALEKRAAALQQDAAALRRERRRLERQADRARALKKDLVRMLTDAGGQ
                     PRATDPRLVALQQVLEGAEGVASVSPPSVNETGGAAVFALTATTAPSDPVTSDLVTRL
                     RTSVIPDATRGEGVTAYVGGITAAYDDLATLISDRLPLVIGVVLALSFVVLLAAFRSV
                     LVPLKAILCNLLAVGAAFGVLTVFFQWGWGLELIGLSGITTVPIASYVPLIMFAVLFG
                     MSTDYEVFLISQIFHAHTEGMDTPTAVRVGVGSSARVITAAAVIMVTVFLSFVTTADP
                     VLKEFAVGLSVAILLDATIVRLVIVPATMVLLGEWNWWLPRWLQWLPRVDLPDERAGA
                     AAPAGPSPAPQTATAGD"
BASE COUNT         2358 a         6408 c         6335 g         2591 t
ORIGIN      
        1 ccggcgaggt cgacgccttc tgggagctcg acctctcccc gtgggaccgg gccgcaggag
       61 cgctgatcgc gcgtgaggcc ggggccgccg tcgagctcct gcccgcggcg cacggctccg
      121 ggccggccgt ggtggccgcc catccctcgc tcctgccgtc gttgctggag ctgctgcgcg
      181 aggcgggcgc gctggcatag cggggctgcc gcggccgcgg agccacccgg aggcgcggcc
      241 gccgcgggat cagccgcgca gctcgagcag gtcgagcggc gcggcgatga ccgccgcggc
      301 cccgctctgc atcagctctt ccggcgtgcg gaacccccac gccgcgccga cggcgatcat
      361 gccggcagcc gtcgccgtgc gcatgtccac gagagagtcg ccgaggtacg cgacgcgccc
      421 gggaggcagc ccgaagcggg cggcgatcgc cagcgccacc gccgggtcgg gcttgagcgg
      481 cgcgtccggt cgagcgccgg cgacgtgcac gaagtcggcg gggtccagca gcgccgcgac
      541 gatggcctgc gtcggcgcgt ccgccttgtt ggagtggatc gcgagcggga cgccggcgga
      601 gcgcaactcc cgcaccagct cgggcacccc gtcgtagact cgcgtcttgt tcagggaatt
      661 gcgcccgtag tcctcgatca tccgctcgta gcagcgcgcc acgcgctcgt cgctgcgcag
      721 ctccgccggc agcgactccg tgaccaggtt ccggatgccg tgcccgatca ggtagcggta
      781 ctcgtcgtag gagtgcccgg gcgcgccttc cagcgccagc acgcggtcca tcgcgtccgc
      841 gatgtcctcg agcgtgtcga ccagcgtgcc gtccaggtcg aagatgacgc cgtcgaagcc
      901 ccgtgcgccg gagtctccgg cgccctcgcc gccgccggcc atgcgcggcg tcagcccagc
      961 gccaggatcg cgaagacgag cacgaagatc gcgaccgtct cgacgatgcc gatgacggcg
     1021 atgtagttgc tgaagccctg acccgtctcg gcgagcgcgt cggcgcagct ggcgccgacc
     1081 ttgccctgcc cgtaggccga catgccgatg ccgagcccgg cgacgatgcc gacgccgagc
     1141 agggcccagg ggtcgtatcc cgcgtcggcc ttgctcgcca tggtgatcat caggatcatg
     1201 ccgtagatcg tctgcgagag cggcgccccg acgaaggtga tgagggtgaa cggcgccggc
     1261 ttgccctggg ccgtcagctt cttccacgcc ccgacggcgc cggcgccccc cagatacgtg
     1321 ccgagggcgg agcccgtggc cgccagcgcc atggcggcga ccgccccgat ccggcccatc
     1381 tctgctgcag cttctggact catcggcatc cccttcgtct gtcggccggc ggcgcgggag
     1441 tcacgcgggt cccctcggtc cggcgaacgg tgtgtacagt gtgtggccct gccaggcgag
     1501 gcccttgtgg gtggagaact cgagcgtgtt gagacgcacg gcgtggacca gcacgcccag
     1561 gcccgcgagc acgatgttga gcgcgttggc gaacagcagc agcacgctgg cggcgacggc
     1621 tgtgagcggc gagtcgaagc cgatgctcgc cgccatgtcg ttgaacgcgg cgagcacggc
     1681 gacggacgca tagccgacgg cgaacaggcg gatgtacgac aggatgtcca cgaagttgcc
     1741 gaccagcgtg agcggcagca tggcgtggtc gatccaactg gccttgagct cggccggcgt
     1801 cttcatgaac gccgccacca cgacgacgcc ggtaagcagc atcagcagca ggaagcccgg
     1861 agcgccgcgg ccgaggacgg ccgagcgggc gatgaagaac atcgaccaca cgacgagcag
     1921 ccagccgccc tgcgcgacca ccttggtcca ggccccctca cggatgaacg acaccgcgtt
     1981 ccagacgtgc gcgaaggaga ggtgcaccgc gccgatgagg aagcacaggt cgatgaggtt
     2041 gtcgcggtcg gcgagccagg ccacctggag ctgctccgcg aagcgcggca gcgtcgggat
     2101 gccgaagtag ctgccggaga gcacgcccca gacgagcgtc gcgaagccga cgatgtacat
     2161 catgtggaag acgtgcgcgg ggaccttctt gaggcgccac tgcagcacgg cggtgagcac
     2221 gagcagcagc acggcgtagc cggcgtcgcc gacgatcatc gcgaagaaga ggctgaagaa
     2281 gggcaggatc acccagccgg cgtccgcctc ccagtacccc gggtagatgt gcaggaagtc
     2341 gaagacggtg ctgatgggcc gcacccagcg cgactggtgc agctggacag gcacgtcctc
     2401 gccgggctgc ggatcgtcga gaaggatgcc ccagccgtgc gcggcggcgg cctcccgtat
     2461 ggcgtcctgc tcggctgcgg gcacgtagcc ggagagcacg gagagctctc cggcctcgtc
     2521 catgccggcg cgcacctcca cccagcgcac ctggtcggcc gtggcgcgcg ccagggccgc
     2581 gacggccgcg cgcgcggcgg cgagctccgt gagacgccga gccgccgcgt ccgtctcggc
     2641 ctctgccgcc gcgacctcgt cgcgcagctc ggcgagcgag cgctcgggcc aggggacgag
     2701 ctcgtagtgg ccgccgaggt cgggcagggg gtccgggcgc ccgccgagga cggcgagcac
     2761 gaggcgccga ccgccgcggt cgcgcccgag ttccgtgagc accacgccgt ccggcgccgt
     2821 caggcgctcg tccggtggcg cgccggcgag cgccgtccag acgccggcgc gctggagctc
     2881 gcgcacctcg tccaggtcga actcgccgaa gccggcgtac cgggccagct cccggcggag
     2941 ctccccggcg cgcacggcga gggcgtcgcc gcggtcgagc agcgcgagca cctccgcgac
     3001 ggcgtcctcg gccggcagcg tcgcggcgtc cgcgcgtgtc gtcgcggcgt ccacgggcgc
     3061 ggtcgcggtc accccgcgcg ccggcttctc cggcagcgcg gccagcgccc gctccgcgcc
     3121 ggcgagccgg gcgcgcagcg cctcgagctc ctcgccggcc ggcgggcgcc tgtccgtgac
     3181 gtgcaccaca ccgagtccgc gcagctcgtc cagggccgcg tcgcggtcgt ggaggaggca
     3241 cagcagcgtg agccgcttca tgcggacgat catgcgctcg acttccccga gagcacgctc
     3301 accgtcttgc tcttggccag cttggcgcgc gccacggccg cggtctgggc gtcgcccagc
     3361 gcgatgcgga tcacgcggat gttgtcgagg gtgcggggga tcatcacctt ctcgaacagg
     3421 ttgacgcgct gggacgtgag ccgcagctct tcaccgagca gctcgcgctg ccgtgagagc
     3481 acgtggatct cggcgcgcag gcggacgagc tccccgagca cctcgaccgc gtcgtcgagc
     3541 caggcgggag tcgagaagta gtcgatcgcc ggggggacga agcgcaggcc ggagagcacc
     3601 ggcacgttca ccccggccac gttgaccgtc tccacctcca ggccctcgac acgcacgtac
     3661 tcggtgaggt ccggcggccc cgtcagcagc cccgcccagg ggcgcaggcc ggcgagcagc
     3721 tcgccggcac gctcctctcg gcggcgcagt tcagcctcgg cgcggcgcac ctcacgctgg
     3781 agctgctgct tcttgagctc cagcgtgggc aggaatcgct ggtagcgctg cagcgcctca
     3841 cgctgggcct tgagctcgtt cttggtcttc ttgagcttgg ccatggctca cgtggagacg
     3901 ccggaggccg ccgccggcca gaaccgctcg acgagcgccg tggggatgcc ggtctcctgc
     3961 ggcgcgaagc actcggccag gatccgccag ccgaggtcga gcgcctcctc gagcgggatg
     4021 gtcacggaga ggtccatcag gcgcgtctcg aacagcccgc cgtacttgag cagcttctcg
     4081 tcccaggcgc tcatgcggaa gcccatggac tgcttctcct ggctctcctt gaaccccgca
     4141 tagagctgga tcatcgtgtt catgatcgtg cggtggtcgt cgcgcgtgcg cgcgttgacc
     4201 tgctgcttga ggcggctgag cgacccgaac ggctcgatgc gcccgttgcg caggtagaac
     4261 tgcccctcgg tgatgtagcc ggtgttgtcg gggatggggt gggtgacgtc gtcgcccggc
     4321 atcgtcgtga cggcgaggac ggtcacggag ccggcgccct cgaaatccac agccttctcg
     4381 tagcgcgccg ccagctggct gtagaggtcg ccggggtagc cgcgcgtgga cgggatctgc
     4441 tccatggtca cggcgacttc cttgagcgcg tcggcgaagt tggtcatgtc ggtgagcagc
     4501 accagcacgc gcttgccggc gagagcgaac ttctcggcca cggcgaggca cgtatcgggc
     4561 acgagcaggc actcgacgac cgggtcggcg gccgtgtgca cgtagaggac ggcgcgggag
     4621 agcgctccgg cgtcctcgag cgtgtcgcgg aagtagaggt agtcgtcgta cttgaggccc
     4681 atgccgccga gcacgatgag gtcgacctcg gcgttgaggg cgatgcgggc gagcagctcg
     4741 ttgtagggct cgcccgccac cgagaagatc ggcagcttct gcgactcgac gaggctgttg
     4801 aagacgtcga tcatcgggat gccggtgcgg atcatcgtgc gcgggatgac gcggcgggcg
     4861 gggttcaccg gcggccccac gatggggatg aggtcgtcgt ccagcccggg gccgcggtcg
     4921 cggggcacgc cgccgccggt gaagacgcgg ccgagcaggg cgtccgagaa cggcgtctgc
     4981 gccccgtgcc cgacgaaccg cacctcggcg tcggtgggca ccccgcgggt gcccgagaag
     5041 acctgcatgg tgacgtagtc gccctccacg cggatcacct gcgcctgcga ggagaacccc
     5101 ggcgcctcca cgctggcgag ctcctggttg cccacgccgg tggcctgcac ggtgatcacg
     5161 ttgccggcga tctgcgagat ctgctggtag accctacgca tcggggctca cctcggagag
     5221 catcgccgcg acgcgggtct cggcggcggc gaaggaatca tcgtcccacg ccgcccggtt
     5281 ccagtccctg gtcgcctggg tgagcgtctg gaagtaggag cgcgcagcgt ccttgtccgc
     5341 gaagtccatg tccgcggcga ggatgcgcgc gatcagcgcg aagacgtgcc gctggcgctc
     5401 cgccgacgag gcgccgtcca ccgggtcgaa ggcgtcctgc tgcaggtaga cggcgtcgag
     5461 gtactcggcc ttgagggaga cgaggaagtc ggccatcgcc gtgccctcct cctcgacgac
     5521 cttcatcatc tcctggacct cgtgcccctt ctcgagcacg ctgcgcgcca gcgagagctc
     5581 gcgcgcgggc acgatgctcc cgtacttgct ccagctgtcg agcgggtcga tcgcggggta
     5641 gcggcgcgcg tccgagcgct ggcgcgagag gccgtggaag gcgccgacga ccttgagcgt
     5701 ggactgcgtg accggctcct cgaagtcgcc gccggcgggg ctcacggtgc cgccgatggt
     5761 cacggtaccg accgtaccgt cgttgaggcg cacggcgccg gcgcgctcgt agaaggcggc
     5821 gatgaccgac tcgaggtagg ccgggaacgc ctcctcaccg gggatctcct cgagccggcc
     5881 gctcatctcg cgcagggcct gcgcccagcg cgaggtggaa tcggcgagca gcagcgcgtg
     5941 caggcccatc tgccggtagt actcggcgat ggtcacggcc gtgtacacgg acgcctcgcg
     6001 ggccgcgacc ggcatggagc tcgtgttgca gatgatcacc gtgcgctcca tgaggctgcg
     6061 gcccgtgcgc gggtcggtga gcacggggaa ctcgcgcagc gtctcgacca cctcgccggc
     6121 gcgctcgccg caggcggcga tgacgacgac gtccacgtcc gcgtggcggc tcacgagctg
     6181 ctgcagcacc gtcttgccgg cgccgaacgg gccggggacg cagtaggtgc cgcccttggc
     6241 caccgggaag aaggtgtcga ggatgcgcat cttggtgacc agctgctcgg tgggccgcag
     6301 gcgctccgcg tagcagcgga tggggcgctt gaccggccag cgctgcagca tcgcgacctc
     6361 gagcgcctcg tcgtcggccg tgcgcaggac ggcgatccgg tcggtgatgg tgtactcgcc
     6421 ggccgccgcg acctcggcga ccgtggcgac gccggtcacg ccgaacggca ccagcacgcg
     6481 gtgctcgaag ataccctccg cgaccacgcc gagcgtgtcg ccggcgcgta cgcggtcgcc
     6541 ggcggcggcg cgcggcgcga actcccacac ctggtcgcgc ggcagcgcgt ccgtctgctc
     6601 cccgcgcggc aggaagaagc cgtgccgctc ggcgatgacg ttgagcgggt tctggaggcc
     6661 gtcgaacacc cgcgagagca acccggggcc gagctccacg gagagcatct caccgacgaa
     6721 ctccacggcg tcgcccacct tgatcccggc ggtgctctcg aacacctgca tgtcggcgta
     6781 gcgcccgcgc acgcggatca cctcggcgat cagccgcagg tcgccgagca gcacgtaggc
     6841 gacttcgttc tggctgacgg ccgcgtcgaa ctcgacggtg acgaggttgc cgctgacgcc
     6901 catgacgcgg ccggtgcgga ggctcaaggc aggctccccg cgagcgccgc gtcggccacc
     6961 gcggccgcga cgcggaggcc ttcggcctcg tccgtcgcgg cccacttctc ggccagccgg
     7021 agcttgagcc cgtaggccag caccgcctgg aggccgaagg gcggcgcggc ggcgagctcg
     7081 tcaaggagcg tccagcgcag ccggtccagg gcgagctcgc gctcgcgcgg gtcctcgagc
     7141 tccatggccc gctctgccgc gctcgcgcag cgcgcgtcgt agccggcgaa cgggtgtgtg
     7201 cccgcgtcgt aggccgcgcc ggcgcgcgcc gcgcgcagcc tggcgaccgt ggtgcgcagc
     7261 tgcgtctcgg cgtcgaggta cggccgcagc tccggcgcgc ggacgtcgtg cggccggtcc
     7321 tcgagcaccg ccctgaggtc ctcccggtgg tcctctcgca ggacgcccgc ggaagaggac
     7381 agcagctcgg aggaggagat gcggggtgtg gccgtgagct ccagcgacgg caggctcgcg
     7441 accaggtaca cgtactccac gtcagccctg ctccgcggcg gctcgccgga cgatgtcgca
     7501 caggggaggc ttgagcaccg gcgcaagcgc ctccgcgagc gcctccgcgc ggaagtcgtg
     7561 atagaggttg tagtcgacga aggagacctt gaagccgtcc tcgacgcgct cgtcgggccg
     7621 cagcgtgagg ccctgccgga cgagctcgcg gtacttctcc atgacgatgg cgccgacggc
     7681 gtcgcggtcc tcgggcgaga ggagcacgtc cacgcggctc tcgttcatgt cgtgggcggc
     7741 gtaagcatcg gccaggcgca cgaggatctg ggccatgacg tccggcgtga gcgcgccggc
     7801 cacggtctcg cagagcgtct cgcgcagcac ggactcgatg ctcttctgca gcgacagcag
     7861 gaagtcgcgc gcggcctgct ccagcgccgt gtgcccgcgc tcgaccgccg cggccgcgtc
     7921 cgtctccgcc gcggcgcgca gcgcggcggc ctggcgctcg gcgccggcga cgatgccggc
     7981 ggcgcgctcc tccgcctccc cgacgatgcg cgcggcctcc gcgtcggcgc gctccacgcc
     8041 ctctgcccgg atgcggtcga gaagactctg gagctctgcg gtcatgtgcg gtccctcacg
     8101 cttcggacta cgtatcggag ttcgtatacg aacttaaccc ggaagtcaat tccgcggtct
     8161 tccctcacga agaacgacca gaccctacgc cgctccgtga gggactgcaa cacccctccg
     8221 ggacgccgcg gccgatggtg gatccgcgtc gccttgagac ccggcaggtc cggtgttagc
     8281 gtggctgccg ggtgtgaagg actcataaag aggaatgacc cggcaatcag tcgataggct
     8341 gaggaatggc acgcagacga taccaggatg agggcggcgc gatcggcttc ggaggcctct
     8401 tcggcacctc cttcggctac gtggggacct ccatctactt cgtcctcggc gccatcggcc
     8461 tgtacgcgct cggggtgacc ccgctcctga tcctcatcgt cggcctcgcc ttcgtggcga
     8521 ctgcgtggtc ctacgccgag gggtcggccg ccatgccgga gtcgagcggg gtgaccagct
     8581 tcacgcggcg cgccttcggg ccgctggccg gcttcgccgc ggcgtgggcg ctgctgctgg
     8641 acagcatcgt gctcgtggcc atcgtctgct cgttcatccc ttcgtacctc agcgtgttct
     8701 ggccgcaggt gcaggactgg ccctacgacc tcctcacgag tctcaccgtg ctcgtggtga
     8761 tcgtcgccct gaacctcctc ggcgcccacg cgtcggtgcg cctcaacgcg ctcatggcgt
     8821 tcctcggcgt ggccaccctc atgctcctcg tgatcgtcgg cttcttcgtg gccgtgaagc
     8881 ccggcctctt gtggagtcag gtcgccttcg ggaccgcgcc gacgtggcgc agcctcctct
     8941 acgcgatccc gctggcggtg gccgcgttca tcggcctcga cgccgtctcc agccgcgcgg
     9001 agaccgcgct gcgcccatcc cgcgacgtgc cccgcgcgat caacctggtg ctgccgctga
     9061 tcgtcgcgct ggccgtcggc gtcgccgtcg tggcgctctc ggcgctcccg gtgggcgcca
     9121 ccgtcgtgcc cgtcgacccg tccaccgggc tcacgcgacc ggtggccgtg gtccccggcg
     9181 acgagccggg ggtgttcgtg ctcgccggcg acccggccac gcaggtcctc gtgcccgtgg
     9241 agcagtccgg cgcggggcac gtcatcccgg cgcaggagcc cacgggggcc gtgaccgctt
     9301 cgccggacgg ccccgtcacg cgcctgtacg gcacgctgct cgggagcgcg tacctgcagg
     9361 accccatgat gggcctcgtc gcgatcttgc ccgagagcct gagcgggctc gaggacgtcc
     9421 tgcgtgtgtg gctcgccgtg ctcatctccg tgtcgctgct gctcaccgcg aacgccgtcg
     9481 tcggcgggtc ggcccgcgtc gccttctcgc tggcgcggca ccaccaggtg ccggccgccc
     9541 tcggccgcgt gcacgcgacg cgcatgaccc cctacgtcgg catcgtgctc ttcggggtcg
     9601 cggtcgtcgt gctcctggtc cccaacgacc cgctcctgct gctcggcctg ttcggcttcg
     9661 gcgccgcgct cgccttcacg gccgcgaacg cctccatcgt ggcgctgcgc tacaaggagc
     9721 cttccatctc gcggccgttc gcgatcccgt tctccgtccg cttccgcggc gtcccgctgc
     9781 cggtccccgc cgtcgtcggg gcggcggcca tggccgcggt ctgggtgctc atggtcggca
     9841 cccatgccgc cggcgccgtg gtcggcttcg cctggctcgg cgccggcctc gtgctctacg
     9901 cggcgtaccg gcgcagtacc ggctacgcgc tgctcacgca gccgcgggag gccgccgtgc
     9961 cggccgcggc cctctccgac gtggactacg accgcatcct cgtgcccgtc gtcggcacgc
    10021 ggctcagcga cgagatgatg gtcctcggct gccagctcgc ggcggagaag gacgcggtca
    10081 tcgacgtcct gtacgtgctc gaggtgccga tgaacctgcc gctggacgcg tcgctgccgg
    10141 acggccgggc gcgcggcaag cacgtcctgg acacggcgat cgcggtcgcg cgcgagttcg
    10201 gcgtcgaggc gtggccgcac ctggtcagcg cccggtcccc cggccgggcg atcgtcgaca
    10261 cggctcggga gtgggacgcg gacgtcatca tcatgggcgc cgtccgcagg taccgcccgg
    10321 acggcaagct cgtcggcgac acggtcgcgc acgtcatgcg ccaggcgcgc gcggaggtgc
    10381 tcctgaacct cgtgggcgag gactacccgc tgcaggggtc cgcggccgag tacgacgcgg
    10441 aacaggcggc cacgaaggcg gcgtccacgt cgaccaccgg agcgtgaggg cgggatgcac
    10501 gtgatcatcg ccggatgcgg ccgcgtcgga tcggggatgg ccaccgacct cgtgcgcgag
    10561 ggtcacgacg tcgtcatcat cgacgaggac cctcgctcgt tcgcgctgct gggcgacgag
    10621 ttccccggcg tgcgcgtcgt cggccaggcc atcgactggt cggtcctgcg caaggcgggg
    10681 atcgagggca cggacgcctt cgccgcggcg accgatggcg acaacaccaa cgtcacctgc
    10741 gccctgatcg ccctgcgcgc gttcgaggtc ccgtgcgccg tcgcgcgcgt ctacgatccg
    10801 cgccgcgcag aggtgttcgc gcgcctgggc atccgcactg tctgccccac gtcgggaacc
    10861 acgctgatga tgctcgcggc cgttctcacc tgcccttcca ctcccaggac ggcctgaggt
    10921 gttcgccgtc atcgtcggag gcggcaaagt cgggctgaac gtcgcccgca gcctcacgct
    10981 gctcggccac gacttcgtcg tcgtcgagca gcggcgcacc cggttcgacc tgctccgggc
    11041 cgagctcgag gaccacctgc tcttcggcga cggcgccgag ctggggacgc tcgaggcggc
    11101 gggcatcgga gtcgccgacc tggtcgtggc cgtgaccggc gacgacgagg acaacgtcgt
    11161 catcgccatg ctggccaggc acgagtacgg cgtgccgaag gtcgtcgccc gcgtcaacga
    11221 cccgaggaac cagaagacct tcgacctcct ggacgtggac gccacggtct gcgcgtcgag
    11281 cctcgtcgtc tcgctgatcc agcacgagct ccccgtgcac cagttcgtcc cgctgctctc
    11341 cctcaggcgt gagggcctcg agatcgcgga gatcgccgtg agcgacgact cgcccgcggc
    11401 ccaccttccc gtgggggaga tccccctgcc tccgggcgtg ctgatcggcg cgatcctgcg
    11461 tggaggcgcg gcggtccatc cgggggacgg cgaagtgctg cgacccggcg accagttgct
    11521 ctgcctgctg ccgctccaga tggagcgctc gctcctgagc tcgctgctgc ccgagcggcg
    11581 gccggaagac gttcaggccg agacgtacat cgacgcggga gccggcgaac gtgcctgata
    11641 ccggaacgga cgaggagcga cgatgaggat catcgtggtg gggaccggtg acctcggctt
    11701 ccacgtggcc aagcggttct cggaggagca ccacgacgtg gtcgtggtcg gacgggacga
    11761 cgaggcgctg cactgggcgc aggaggtcct cgacgtcagc gccgtgcgcg ggcacggggg
    11821 ctccccgtcc atcctcgtcg aagccggggt gcggtcggcc gacatcgtgg tagcggcgac
    11881 gagcaacgac gagacgaaca tcgtcgcttg cctcatcgcc gaggcgatcg ccccgacggc
    11941 cacccgcgtc gcccgcctcc acgatccggc ctatctgggg tcgagcggca tcatcgacca
    12001 gagctcgctg agcatcgacg tggtcatcag ccctgaggag gaggtggcca aggcggtcgc
    12061 cgtcctggcg agcgtcccgg gcgccgcgga tgcgctcgag ttcgccggcg gccgcgtgct
    12121 ggcggtgggc gtcgaggtcg acgaggactc gcccatgatc ggccggaccg tcgccgagct
    12181 gcgccggaag gacaaggggg ggctgctcgt ggccggcgtg taccgcggcg aggtggtcag
    12241 cgcccccacg agcgacctgc gcatccaggt acgggacgta ctcttcctgg tcggcggccg
    12301 ggacgcgctg cgccgggcga tgacgcggct gggcaagacg tgggcgcgga cgcgcagcgc
    12361 gatcatcgcg ggcgggggct gggagggcgt cgccatcgcc cgtcagctcg ccgcgtccgg
    12421 catccacgtc aagatcatcg aacgcgaccc ggccgtgtgc gacgagatca cgcgcgagct
    12481 cggcgacgcc gtggtcctgc tcggcgacgc ccacgatcac agcctgctcg tcgacgcggg
    12541 ggtgcagcgc tccgacctct tcgtgtcggc gctcgggagc gaggccgaca acatcgtcgt
    12601 cgctctcctc gccaaacgcc tcggctgccg gcgcgtggtc agcctcgtgg acacccccgg
    12661 ccaggcggcg ttcgcctcgg ccatcggcgt cgacagcgtg gtcagcccgg tgctcgccgc
    12721 cctcaacacc atcgtccagc tctcccggcc gggccaggtg gtcaccgtgc gcaccctgcg
    12781 cgagagcctg gtcgagggca tcgagttcgt ggccatggag ggttcgggga tcgtgggcct
    12841 gcagctggcg ctgctgcgca tgccgcatgg gtcgctcatc ggggcgatcg tccgcgacgg
    12901 caaggcggtc atccccgaga gctcgaccgt cgtgaacgcc ggcgaccgcg tgatcctctt
    12961 cgcgcggccg gcgctcctcc cgcgcctccg ccggctcatc accgccgaat gagcgaccgt
    13021 ccgcagcgat gacgcggcgc tcctcgtacg ccggccgggc ccgtgtccac ccgacgacgc
    13081 tgtcctcggc ccggcgcgtc ggctatctgc tcagcctgct gctggtcttc gccagcctga
    13141 ccctggcggt cccgctggcg atctccgcgg gctataggga gccggagacc gtcggggact
    13201 tcgcggcaac gctgctcatc ggggtggtga tcggcctggc cggctacctc ttccttcgga
    13261 ccgacctggc gggcctcaca cggcgcgagg ggttcgccgc ggtcgcgctc ggctggctca
    13321 tggtctgcgc gctcggatcg ctgcccttcg cgctcgccgg cggcctcgac ccggtcaacg
    13381 cctggttcga gacggtcagc gggttctcgg gcacggggtc gagcgtcatc cccgacgtcg
    13441 agtccttgcc gaagggtctg ctgttctggc gcagcttcac gcactggctg ggcggcatgg
    13501 gcttcgtggc gctgtacatc gccatcttcc cgctcatggg cgtcggcgcc atgcagctct
    13561 tccgcgccga ggcgcccggc ctcgagatgg accgcctgcg gccgcgcatc cgcagcaccg
    13621 cgcgcatcct ctggctcatc tatctgggca tgtcggtgat cctgaccgcc ctgctgatgg
    13681 ccggcggcat gggctggttc gacgccgtgt gccacatgtt cgcggccatc ggcaccgggg
    13741 gcttctcgac gaagaacgcc agcgtcggct ggttcgacag cgcgtacatc gactgggtga
    13801 tcatcctgtt cctgtggatc gcctccacca acttcgcgct ctggtgggcc gtcctcggcg
    13861 gcaagccgaa gcggttgctc aaggacccgg aatggcgctt ctactcgctg gtgctgctcg
    13921 gatcgagcgc ggtcgtctcc gcgctcgtct atctgcagaa caacgtctcg ctccacgacg
    13981 ccatccgcaa cgccaccttc gaggtcgtct ccacggcgtc cacgaccggc ttcgcgaccg
    14041 ccgactacga gaagtgggcc cccctggcgc agctgatcct cttcctgctg ctgttcatgg
    14101 gcggctccgc ggggtccacg gcgggcgcca tgaaggcggt gcgcgtctac ctgttcgcga
    14161 aacaggcgct gcgtgggctc ttcaccacca tccacccgca gtcggtggcg cccgtgcggc
    14221 tcggcgggcg ggccgtgtcg ccggaggtga tgcgtggcat ctcgaccttc atgggtctct
    14281 acctgttgac ctacgtcgtc gcggtggcgg ccatctccat caccgacatc gacttcgcca
    14341 ccgccatgtc cgcggtggcg acggcgatgg ggggcgtcgg gcccggtctg ggcgagatcg
    14401 gcccctacga caacttcctc tggatgcacc cgttcgccaa gctggtgctc tcgttctgca
    14461 tgctggccgg ccggctcgag ctgttcacgc tcatgctgat cttcgtgccg gcctactggc
    14521 gccgctgact ctcgccgccg gccgcaccgg cgcgatgctt gtcgcggcgg ctgcggtgcg
    14581 gttcactagg cacccagccg tcacacagtc caaccgggag gttccccatg tgcttgcgcc
    14641 tcgaaggcta cgtcaccatc gtcaccggcg cggcgtcggg catcggcaag gcgaccgcca
    14701 tccgcttcgc ccaggagggc gccaaggtca cctgcgtcga catcgacggc gacggcgtca
    14761 aggcggtcgc cgaggcgatc ggcggcgacg cgttcggcgt gcaggccgac atctccgacc
    14821 cggagcaggt caaggcctac accgacggca ccgttgaacg ctggggcaag ctcgacgtcg
    14881 ccttcaacaa cgccggcgtc aacctccccg gcgtgttcca cgaggtcccg gacgaggtca
    14941 tcgaccgcac cctcgacgtc aacgtcaagg gcacgatgtt cgggtgccgg tacgcgatcc
    15001 cgcacatgct cgagcagggc aagggatcgc tcatcaacac gacctcggtc aacggcatcg
    15061 tcgccgagcc gtacctcacc gtgtacgccg cctccaaggg cgcgatcgtg atgctctcca
    15121 agggcatcgc gctcgactac gtcaagaagg gcatccgctg caacgccatc gccccgggct
    15181 gggtcgacac gccggtgaac tacgcccacg cggagatgct cggcggcctg cagcacgtgt
    15241 acgacacgat cgactcgttc cagccgatcg ggcgtcccgg cgagccggtc gagatcgcca
    15301 actgcgcgct cttcctcgcc tcggacgagt cgtcgttcgt caccggcacg gtgctggtgg
    15361 ccgacggcgc gatgacggcg cagtaagggg cgcggcccgg cctcacccca cccaggcctg
    15421 gtcgtcccag aaggcgacga ccttgcgccg gcgccctcgt agctcgagca cgagctcgtc
    15481 cggcgggcgc cgctcgcccg ggtcgaagag cacctggaag ccgccggcgt cgtgggcgcg
    15541 gaactcccgc gccccgccct ccggcggcac gctgccggtc tcgagcagga ccagcgggcc
    15601 ggtgcagcag cggtgcgcgc tggtccacac gtagaggcgg ccgccccgct cccgcacggc
    15661 ggccgccacc tcgtctgtcg cccggatcct catgtccggg gtgttccccg caactccgcg
    15721 cgccaatgca cgacccaggg cgcgccgcgc cgcacccccc agcccctcag tcgcccgccg
    15781 tcgccgtctg gggggcgggc gaagggccgg ccggcgcagc cgctccggcc cgctcgtccg
    15841 gcaggtccac cctgggcagc cactgcagcc agcgcggcag ccaccagttc cactcgccca
    15901 gcaggaccat ggtcgccggg acgatgacca ggcgcacgat cgtcgcgtcc agcaggatgg
    15961 cgacggacaa ccccacggcg aactccttga gcaccgggtc cgcggtggtg acgaagctca
    16021 ggaacacggt gaccatgatg acggccgcgg cggtgatcac gcgcgcgctg ctgcccacgc
    16081 ccacgcgcac ggctgtgggc gtgtccatgc cctcggtgtg ggcgtggaag atctggctga
    16141 tcagaaagac ctcgtagtcc gtcgacatgc cgaacagcac cgcgaacatg atgagcggga
    16201 cgtagctggc gatcggcacg gtggtgatcc cgctgaggcc gatgagctcg agcccccacc
    16261 cccactggaa gaagacggtc aggacgccga acgcggcgcc cacggcgagc aggttgcaga
    16321 ggatcgcctt gagcggcacg agcaccgaac ggaacgccgc cagcaggacg acgaagctga
    16381 gcgcgaggac cacgccgatg accagcggca gacggtcgga gatgagcgtc gcgagatcgt
    16441 cgtacgcggc cgtgatgccg ccgacgtagg cggtcacgcc ctcgccccgg gtagcgtcgg
    16501 ggatcacgct ggtgcgcagc cgcgtgacca ggtctgaggt gacggggtcg gacggcgccg
    16561 tcgtcgcggt gagcgcgaag acggcggcgc cgccggtctc gttgacgctg ggcggcgaga
    16621 cggacgccac gccctccgct ccctcgagga cctgctgcag ggccacgagg cgcgggtcgg
    16681 tggcgcgggg ctgcccgccc gcgtcggtca gcatccggac caggtccttc ttcagggcgc
    16741 gcgcccggtc ggcctgccgc tcgagccgcc gccgctcgcg cctcagggcg gcggcgtcct
    16801 gctgcagggc ggcggcccgc ttctcgagcg cgcccccctc cttggtcagc cgcgcgccct
    16861 cggcctgaag cgcgtcgccc tgcgccgtga gcgcggcgga ctcggcctgc agcgccgcgc
    16921 cctcccgttc caggcgtcgc ccctccgccg cgagctccgc gccccggtcc tcgagcgccg
    16981 ccacctcctg ctgcagggcg gcggcgtccg cctggacccg ggcgatctgc tgctgcacct
    17041 gctgcgcctg ggccgccagg gtgacgagct gcgtctgcag ctcggccagc tcggccgggt
    17101 ccgtggtcgc cgcgatctgc gcctgcagcg cggcgatctg cgcctggagc tgctggccct
    17161 cggcggcgag cgccgcgccc tgctgctgca gcgcggcgcc ctcggccgcg agcgcggcgc
    17221 cctgggcctc caggcgcgcc ttctgcgccc gcagccgctg ctcccgcgcc agcagggcgt
    17281 ccctgcgcgc gagcagcggg gccgcctgcg cctccagcgc ggccttgcgg gccgtgagcg
    17341 cggccgcgcg ccgctccagt ggcgccttct ccgccttgag cgcgtcgccc ctccgctcca
    17401 gcgccctgcc ctgccgcttg agccgcccgg cgtcctgctc gaggctctcg gcgaggcgct
    17461 ccgcccgagc cttcttccgc gtgtacgccg cgctcggctc ggcgacgggc gagaaccgcg
    17521 cggcgatcag cagacgcccg ttggcgccgg cgccgagccc ctccgcgatg aggtcgtagg
    17581 cgcggcgctg ggtggtcgtc gtgggccagg cgccgatgct ctcctgcccc aggatcaggg
    17641 tgagcgtggg ggccgcgagc ggcaacagca cggcgagcga ggcgatgagc gc
//
LOCUS       DASUMX010000145        19336 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_2057799, whole genome shotgun sequence.
ACCESSION   DASUMX010000145 DASUMX010000000
VERSION     DASUMX010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 19336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19336
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2057799"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..1002)
                     /locus_tag="VFZ86_07020"
     CDS             complement(79..1002)
                     /locus_tag="VFZ86_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005517774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEX5642079.1"
                     /translation="MAYVYPTTWFGDGLAPAPDASLSITSAAVLYGLSVYTVLPVTVS
                     GDRAPLAFRLADHYRRLVNSARIIGIDTFEPAWGEERFAAAVTEVVLANRVTEDVFVR
                     ATVHVDAELPGTRSRGMSTRLSIFVYTAEPILPLDGARLKTSVWRRIPDYAIPSRSKV
                     NGAYVNSVLAKQDAIDSGYDDCIFLDAAGHVCELSAANIFIVREGTLVTPSTASDLLE
                     GINRSTVLLIAEHLGIPTREREVDLTELYIADEVFACGTSTYVTPVTEVDARTVNGCR
                     VGPITERIRDRYLAVLRGEDAYYRRFVTRLG"
     gene            1117..2523
                     /locus_tag="VFZ86_07025"
     CDS             1117..2523
                     /locus_tag="VFZ86_07025"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX5642080.1"
                     /translation="MKIRLIEPAPAHLHMWSYTFLPRLGLPLIGAGLEAAGHDVVIYC
                     PQFSPIDWDDVLGADLVGISTTTSTAPAAYEIAARVREHGVPTIVGGSHVTFMADEAL
                     AHADYVARGEGGDTLMLELIEVLRGERTLETIDGLSFTRDGEAVHNPPREACRDLDTL
                     PVPDLTLIAGSRDRRGTTPIMTSFGCPFGCNFCSVTAMFGRKYRYRSPEHIMAEIEAK
                     QPRSIFFYDDNFAADKRRLKTLLRMMIARGRKIPWSAQVRTDVTDDPELLELMRDSGC
                     DRVALGLESVSQETLDGFDKSQSVADIVRALKALRRYGIKTHGMFVLGADSDTAATVR
                     DTARFARKHDISTLMLNILTPLPGTPLFDELDAQGRIFDRHWDLFDGQHVVFSPGQMS
                     PETLQAEVWRAYARFYSFRQWLKYLATFRYGTMALYSWYWVFLRRWRKERGNRDYLEM
                     LKRLHLGGPTPAKADTQG"
     gene            complement(2547..3179)
                     /locus_tag="VFZ86_07030"
     CDS             complement(2547..3179)
                     /locus_tag="VFZ86_07030"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5642081.1"
                     /translation="MTGQEAAGGRRAALHARTRDEILDAATELVTEHGVEGLNLQDLA
                     TRAGFGNAASLYRYFAGKQEIVGALAVRGLERLGEHLRSVPEDLPPEEQIVEICLAYL
                     EYARRHPGERRLLLTTAASIAPDYRAAALPDEIVGRMFRLAEAARASGTLNVRDEDDV
                     FAILHAGWALAHGMAEYDSLYEDPERGLLRNRHRAVFKAYVAGFGSDWTD"
     gene            complement(3176..6415)
                     /locus_tag="VFZ86_07035"
     CDS             complement(3176..6415)
                     /locus_tag="VFZ86_07035"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX5642082.1"
                     /translation="MTIGLWLVALVMAGGVYLTWGDVFTSSSKFLNEPDSEKAVRLIE
                     EHGGGGGGSALAQTGASVQSLADGLDDARKGARQLARGSGSVSSGAKELQRGLRKLAR
                     GAGEVSSGTERARSGAQSLSNGLAGASQGAASLSSGLAEVDGATGSFSAGLQQLSQGG
                     DRLAAAAGKVASGASDLAGGADGAAAGVGKAAASAEQIAGGAATLDQLVAAYLQAHPE
                     VADDPAFQQIMGLSGQLVSGTSGLSSGLQSASAGVSSVAAGAGRLASGASSLAAGARA
                     LDAGLARSASGARQLDRSMGRLASGSASLSSGIGSAASGSQELAAGSRQLAAGSGKVA
                     SGVSGAAAGAGELASGTDAVRAGASELSRGLDSASAGAGQLSDALSSAGTLNNHDSEV
                     VVIHSDGLTVDDPAFRREVVRIRDQIDALPEADVVTVLSVFDEGLDEETRDALTSDDG
                     HTTIMKVELAAAADEAVNHVDGVYAVVEEADAQPEFEVAVTGAASLMKDAQDLAEKDL
                     RRGEAIGIPVALVILVVVFGALVAAGLPLVLSIFSIVVGLAITVLIGQNFELSIFALN
                     ILTATGLAVGIDYSLFIVSRYREERHGGLDEMAAIGAASATASNAVFFSGMTVVLSLV
                     GMLIVPLSIFVSLGVGAMSAVFAAVAAALTLLPAILALLGDKVDALRVPWLERYARRH
                     SEHGWWGRTARRVMRRPAVSLALGAVLLLAIAAPALTMRSGGFSADAFPQSYTSKRGL
                     DMLKSDFAAGMSEPTTVVVDGDLGSEKVQGSIERFVDRLDEDGRFTVTGFSTSADGSL
                     AVIQMIQDADAMSERAKVVVHDLRDTLAPESFAGTGAEVYVGGTTAVQIDSVDLTDGY
                     MPIVIATVLLLSFILLLLAFRSVVVAATAILMNLLSVGAAYGALTLVFQHGWGAEVVG
                     LTRMEAIESWVPLLMFCVLFGLSMDYQVFLLSRIRERWTEAHDSGEAVVFGVQSTAGI
                     ITGAALIMAAVFLGMGSGRLVVLQELGFGLAIAVLLDAFVVRVIVAPALIALIGDRYW
                     WMPRWLEWLPRIDIEGPAPAGGAGGDDATKPALTGARGAGAYTDAGGGI"
     gene            complement(6768..7349)
                     /locus_tag="VFZ86_07040"
     CDS             complement(6768..7349)
                     /locus_tag="VFZ86_07040"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-hydroxy-2-methyl-2-butenyl 4-diphosphate
                     reductase"
                     /protein_id="HEX5642083.1"
                     /translation="MTADHARLLVIAPLRIERAALRRGLPDARVLCSGMGAARARAAA
                     RRAASAPADAVAVAGFCGATVAGLRAGDVVVAGEVRGPDGVTGCDSLPLIEALAALGI
                     ERIHAGPLASVGHVVRGAERGVLAREGVLAVDMESAWLAPAAAGRPFAVLRVVLDTPA
                     SEFRRPWAMLSGAVTAWMALRRAAPALALWARR"
     gene            complement(7415..7972)
                     /locus_tag="VFZ86_07045"
     CDS             complement(7415..7972)
                     /locus_tag="VFZ86_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012890868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX5642084.1"
                     /translation="MRPIIAGLFTSLDGVVEAPEKWQSPYFDNEMGQEMGQAMAEADA
                     LLLGRRTYETYAQAWPNAEGPVADFMNGVPKFVVSTTLESVGWQNSTLIKGDPGVEIA
                     ELKRRPGRSILVNGSATLLRSLVRYGLLDELRLMVCPVVVGEGRRLFDGWNAQVPVKL
                     VRSQRFGTGVLALDYRIRREAEHSA"
     gene            complement(7999..8718)
                     /locus_tag="VFZ86_07050"
     CDS             complement(7999..8718)
                     /locus_tag="VFZ86_07050"
                     /inference="COORDINATES: protein motif:HMM:NF017959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HEX5642085.1"
                     /translation="MNDYQVARPSGALSATTSGRTLRLLVCGVVAGPLFVAAALIQAL
                     TRAGYDLGSQPISLLSLGELGWVQIATFVVSGLLVAAFAVGARRVLKGSRGGTWGPWL
                     LGAFGVGLIAAGVFVADPSLGFPPGAPGGTPEQLSWHAMLHGAGFMLAMGSAGIACLV
                     FARRDVAQGRRGWAAFALATAATAIALSMWPGQGGASIRYFVAAVIVWAWVSATALRL
                     GLGLTRRVAEPADVERRRRAA"
     gene            8833..9543
                     /locus_tag="VFZ86_07055"
     CDS             8833..9543
                     /locus_tag="VFZ86_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008835418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5642086.1"
                     /translation="MAGPPPTAEEPREGPERPPEGRSARKHRAILSAAGELFLRQGYL
                     GTSVDEIAASAGVSKQTVYKHFDDKQHLFTAVVFASIDHVGRPFYEEIHRLEDSDDLE
                     ADLRELAKRLSAVATDQRLLQVRRLVIGEAARFPELGHAYYERAVDRTTRSLEARFRG
                     LAERGLLRLDDPYLAAQTFIWLVLSVPLNRAMFSPETETDAAGLRRIADEAVRIFLAA
                     YGATPASPRRGARPPAQA"
     gene            complement(9540..10733)
                     /gene="dinB"
                     /locus_tag="VFZ86_07060"
     CDS             complement(9540..10733)
                     /gene="dinB"
                     /locus_tag="VFZ86_07060"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEX5642087.1"
                     /translation="MPRAILLIDMDAFFAAVEQARRPGLRGRPVIVGGRAESRGVVST
                     ASYEARACGVRTAMPMAQALRRCPQGVFLPVDMAAYKAVQQRLLAIYGRFTDLVEPVS
                     IDEAFLDVTGSRRLFGSPQEIAGQIQRLTREELGLSCSIGIGPTRLLAKLAAELDKPG
                     GLTTLTRDDVHGRLRSMPVGAVSGIGPVTVKRLSSLGITSIGDLQDAPLPLLEASFAK
                     TAGSLRELAFGGDDAPVRAGHAAPKSMGHEVTFAEDTADPEFLRATLLEQADQVASDL
                     RRQGYAGRTVVLKLRDARFHTVSRRRTLPAPTNTTQVIYEAALTLFAELHQPGRLLRL
                     VGLTLGGLTEARQTALDDSARDHACDEAVDRVRARFGSQALRRAGGGLASGPDQASPD
                     QRPES"
     gene            complement(10766..11701)
                     /locus_tag="VFZ86_07065"
     CDS             complement(10766..11701)
                     /locus_tag="VFZ86_07065"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5642088.1"
                     /translation="MAEQEPKDRYDVVVIGGGPAGLAAAIYTSRQGLSTAIIAGELGG
                     QAQWASHVENYLGFELIAGSELADRFRAHAAGFAIDTFEGRYADAVVPSDALFEVHSR
                     EGLQLRGRAVIIASGRAPVRLSVPGERELVGRGVSYCAYCDAAFFRGRPVAVVGPGES
                     AAEAALQLAALDADVVLVSEQELRAPQTLLSKLEADPQVEMRTGLQARRIEGEDHVTA
                     LVVQDDAENELVLPVDAVFIEAGTIPVGEFTGGLVRVNAAGEVEVGRDLMTSRPGIFA
                     AGDVTDAPGKQIIIAAGDGARAGVSAARWLQRQQT"
     gene            complement(11879..14260)
                     /locus_tag="VFZ86_07070"
     CDS             complement(11879..14260)
                     /locus_tag="VFZ86_07070"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HEX5642089.1"
                     /translation="MLFLLLAAVALLAFAGVAAAAEPSTVGNWWDVTPEAEVPSPQYD
                     SILYSEIAPRLRQIEMASNRVKVEVIGQSAGGRNLFLVTISDPQAQGRLGQYQAIRQA
                     MIRDPEHAQELIDRFGDFKVPVFINGSIHGNEYPGTDAAMRLIEEFAFSNEPEVLDVL
                     ENEILLINVCQNPDGRVLGTRQNANAIDINRDFITQSQPETRATVGVFTEWNPMVVLD
                     LHGFVNPMLIEPCTPPHNPNYEYDLYIKWALAQAEAMEAELFAQTGFSAQIPYRDDPL
                     EWDDWPPTYAPMYAMFHGAYGHTMETPYRDARGVDAHYAAVWGALDFVAENRVGMVRD
                     QVEIFRRGFLSLPQQPIPPELLPQWPQYQDVIPNGFPAAYVIPADEPFQISPQAPARL
                     VDFLLFNDVQVEKASKGFTLDGVAYPAGTYVVWLDQPKRGLANTILSDGLDLSAIPGL
                     EFYSPPTVWSHPLLWGVRRAVMEDPMALQTRAIAKADAPQGTVQGGKAGAYAYLPTSI
                     AAIQATNDLLARGVAVSRAPASFTDGGRAFDAGAFILPGTAKTVANELASRYGLDAYA
                     LRGVPAGAIALRRQRIAVAFSDAGGRELLKRLGFAYDVITVSTLNDATKPPLTEYDVF
                     INTSVHFNTGGLNATGKARLTDYFAAGKDYIGIGGTGALLATQMLPSFVFTATSYTGG
                     PLDSAIRVDYNVNDPVGSGFGAQGYGFANGTRTFSGLAADVRVSATIHDPDFLVSGYW
                     PDWQTSGAAGKPVVIGWASGTQDAALIGLDPIFRGHPEDSFRLVANAIYSGLD"
     gene            complement(14479..15369)
                     /locus_tag="VFZ86_07075"
     CDS             complement(14479..15369)
                     /locus_tag="VFZ86_07075"
                     /inference="COORDINATES: protein motif:HMM:NF022112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HEX5642090.1"
                     /translation="MALAVLALLALAAPLVLAACGTEETPPVSDGTPAATQPATETPA
                     ATETPAASPSPPETAAGETTVLVYFLRGEELGTAERTLPKTVAVARAAMEALVAGPNG
                     DEKAAGLGTAVPKGTRLLGISADGAVATVDLSREFASGGGSLSMTARVAQVVYTMTQF
                     KTIRAVEFMLDGERVATIGGEGVIVETPQRRADWQSFEPAIFVERPGVGATLSSPFIV
                     QGRATVFEATVNIDLLDAGEQTLVETFTTATEGAPGRGAFRKKIVYSTGSDSGWLVVY
                     EASAKDGSHLNEVRIPVSLL"
     gene            complement(15570..17006)
                     /locus_tag="VFZ86_07080"
     CDS             complement(15570..17006)
                     /locus_tag="VFZ86_07080"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX5642091.1"
                     /translation="MKIRLIEPAPPSINILSYGFYPRLGLPLIGAALKAAGHDVLIYA
                     PQAAPIDRADVASADLVGISTTTSTAPTAYALADELRAAGLPVIIGGPHPSFVPDDAM
                     PHADYVARGEGGDGLMLELLEALRGERELDSVNGLSFWRDGLPVHNELRERCADLDSL
                     PMPDLSLIVGSERMRETPIMTSLGCPFDCTFCTVTMMFGRKYRMRSPESVIAELEAKK
                     PRSVFFYDDNFAADKRRLKKILQLMIDRGITLKWQAQVRTDVARDEELLTLMERSGCH
                     RLALGFESVDQATLDSYAKSQTVEDITNAIAALHRHGIHVHGMFVVGADSDTVRTAGA
                     TVAFAERYRIDSLMLNILTPGIGTRQYEMMNGDERVFERRWQFYDGQHVIFEPQNMTA
                     GQLQTGVIDGYRRFYSVGNIVRPLARLRLGTSLERLWGRLYIHRWQKDPTNRAYVGAL
                     AERSAGAAGAGRPAPEPVACIREGSAGG"
     gene            complement(17202..17408)
                     /locus_tag="VFZ86_07085"
     CDS             complement(17202..17408)
                     /locus_tag="VFZ86_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012370557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX5642092.1"
                     /translation="MATGKVKWFNADKGYGFIEHEGGDDLFVHFSEIQSEGFKSLDEG
                     QAVSFEQGTGKNNKPQATNVRVLS"
     gene            17674..17847
                     /locus_tag="VFZ86_07090"
     CDS             17674..17847
                     /locus_tag="VFZ86_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642093.1"
                     /translation="MKSRVAGGFILPSTGKLSDRIKSYGSGRICEARGCTTILSAYNP
                     AEFCSTHKRAGTR"
     gene            complement(17924..18454)
                     /locus_tag="VFZ86_07095"
     CDS             complement(17924..18454)
                     /locus_tag="VFZ86_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642094.1"
                     /translation="MKVPLTWVWENDPHDLPDDDHARCIAAPGERFVACGGQLFALTT
                     RPHARRSASRRAASFSTLQMEYLGLDWPRGLAQQYWGCEPFPSGPLDSGPLDSEPLAG
                     DAQQAAGIALLATVAGALAAGSAMRSGSSSSSRVGFDDLRPPPRPPGWPPRRHARSAP
                     RPALLPLTVGRSGDRR"
     gene            complement(18548..>19336)
                     /locus_tag="VFZ86_07100"
     CDS             complement(18548..>19336)
                     /locus_tag="VFZ86_07100"
                     /inference="COORDINATES: protein motif:HMM:NF024585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX5642095.1"
                     /translation="SYVERADCLAVTNCFCRHTATHLGTACDYPLETCMSLGVAARYL
                     IRHGHARQIGREEGRELLEAAREHGLVHIADNVQRELSYICSCCSCCCVELRSAQNNM
                     AVMQPSAFQPAVDDHRCTGCGRCVRACPVQALSLVPRQGARQNDGDDAATPLTSRVDL
                     ARCIGCGVCVGACREDAVTMVRRPRAPHVPLNAVEYLTRRMLERGRLADLLVDGTAGR
                     GPAFANAVLGAILSLPPAAKLLASEQVQSRFVDFALARYQPPRG"
BASE COUNT         2923 a         6837 c         6778 g         2798 t
ORIGIN      
        1 ttgctcaggc aagtatgtat tccctcaggc gggcagaagg acacccgggc agcggcgacg
       61 caggcgcgcg gagcagcctc agcccaggcg ggtgacgaag cgccggtagt aggcgtcctc
      121 accgcggagg acggccaggt accggtcccg gatgcgctcc gtgatgggac cgacgcggca
      181 gccgttcacg gtgcgcgcgt ccacctccgt gaccggcgtc acgtaggtcg atgtgccaca
      241 ggcgaagacc tcgtcggcga tgtacagctc ggtgaggtcg acctcccgct cccgggtggg
      301 gatgccgagg tgctccgcga taaggaggac cgtgctgcgg ttgatgccct cgagcaggtc
      361 gctcgcggtg ctcggggtga cgagcgtccc ttcgcgcacg atgaagatgt tggcggcgct
      421 gagctcgcag acgtgtccgg ccgcgtcgag gaagatgcag tcgtcgtagc cgctgtcgat
      481 cgcgtcctgc ttggcgagca ccgagttgac ataggcgccg ttgaccttgg agcgcgaggg
      541 gatcgcgtag tcggggatgc gccgccagac gctggtcttg aggcgcgcgc cgtccagcgg
      601 caggatcggc tccgccgtgt agacgaagat gctgaggcgc gtgctcatgc cccgcgaacg
      661 cgttcccggc agctccgcgt cgacgtgcac ggtggcacgc acgaagacgt cctccgtcac
      721 gcggttcgcg agcacgacct ccgtcaccgc ggcggcgaag cgctcctcgc cccacgccgg
      781 ctcgaaggtg tcgatgccga tgatgcgggc ggagttgacg agccgccggt agtggtcggc
      841 gaggcggaag gcgagcggcg cccgatcgcc cgagaccgtg accggcagga cggtgtagac
      901 gctgagcccg tagagcaccg cggcgctggt gatgctcagg gaggcgtcgg gggcgggcgc
      961 gaggccgtcg ccgaaccagg tggtgggata cacgtaggcc atggcgcaag gtcctaccag
     1021 aagcgcgcca ccgagggcaa cctctcgccg cggttgtctt tgcgtcctct tgccgtactc
     1081 tggaccgaca caacgcaccg agaaagagac ccccgcatga agatccgctt gatcgagccc
     1141 gcccccgccc acctgcacat gtggtcctac accttcctgc cgcgcctggg cctgccgctg
     1201 atcggcgccg gcctcgaggc ggccggccac gacgtggtca tctactgccc gcagttctcg
     1261 cccatcgact gggacgacgt gctgggggcg gacctcgtcg gcatctccac gaccacgtcg
     1321 acggcgccgg ccgcctacga gatcgcggcg cgggtccgcg agcacggggt gccgacgatc
     1381 gtcggcgggt cgcacgtgac cttcatggcg gacgaggccc tcgcgcacgc cgactacgtc
     1441 gcgcgcggcg agggcggcga cacgctcatg ctcgagctga tcgaggtcct gcgcggcgag
     1501 cggacgctgg agacgatcga cggcctctcg ttcacgcgcg acggagaggc cgtccacaac
     1561 ccgccgcggg aggcgtgccg cgacctggac acgctccccg tgcccgacct cacgctgatc
     1621 gccggctcac gtgaccgtcg cggcaccacg ccgatcatga caagcttcgg ctgccccttc
     1681 ggctgcaact tctgctcggt gacggcgatg ttcgggcgca agtaccgcta ccgcagcccg
     1741 gagcacatca tggccgagat cgaggccaag cagccccggt cgatcttctt ctacgacgac
     1801 aacttcgccg ccgacaaacg ccgcctcaag acgctgctgc gtatgatgat cgcccgcggg
     1861 cgcaagatcc cgtggtcggc gcaagtgcgc accgacgtga ccgatgaccc ggagctcctc
     1921 gagctgatgc gcgactccgg ctgcgaccgc gtcgcgctcg gcctcgagtc ggtgagccag
     1981 gagaccctgg acggcttcga caagtcgcag agcgtcgccg acatcgtccg cgccctcaag
     2041 gcgctccgcc gctacggcat caagacgcac ggcatgttcg tcctcggcgc cgacagcgac
     2101 accgccgcga ccgtgcgcga caccgcccgg ttcgcgcgca agcacgacat cagcaccctg
     2161 atgctcaaca tcctcacgcc gctccccggc accccgctgt tcgacgagct ggacgcccag
     2221 ggccgtatct tcgaccgtca ctgggacctc ttcgacggcc agcacgtcgt cttcagcccc
     2281 gggcagatgt cccctgagac cctgcaggcc gaggtctggc gcgcctatgc ccgcttctac
     2341 tcgttccgcc agtggctcaa gtacctggcg acgttccgct acgggacgat ggcgctctac
     2401 agctggtact gggtgttcct gcgtcgctgg cgcaaggaac gaggcaaccg cgactacctc
     2461 gagatgctga aacggctgca tcttggggga ccgacgccgg ccaaggcgga cacgcagggc
     2521 tgaccggtgc ggccggcgag ccggccctag tccgtccagt cgcttccgaa cccggccacg
     2581 taggccttga acacggcgcg gtgcctgttc cggagcagcc cgcgctcggg gtcctcgtac
     2641 aggctgtcgt actcggccat gccgtgggcg agcgcccaac ccgcgtgcag gatggcgaac
     2701 acgtcgtcct cgtccctcac gttgagggtg ccgctcgcgc gcgccgcctc tgccagccgg
     2761 aacatgcgac ccacgatctc gtccggaagc gccgcggcgc ggtagtcggg cgcgatgctc
     2821 gcggcggtgg tgagcagcag ccgccgctct ccggggtgcc ggcgcgcgta ctccaggtag
     2881 gcgaggcaga tctcgacgat ctgctcctcc ggaggcagat cctccgggac gctgcgcagg
     2941 tgctcgccga gccgctcgag cccgcgcacg gcgagcgcac ccacgatctc ctgcttgccg
     3001 gcgaagtagc gatagagcga cgcggcgttg ccgaacccgg cgcgcgtcgc caggtcctgc
     3061 aggttcagcc cttcgactcc gtgctccgtc acgagctcgg tcgcggcgtc caggatctcg
     3121 tcgcgggtgc gggcgtgcag cgctgcgcgc cggccgcccg ccgcctcttg cccggtcata
     3181 tcccgcctcc cgcgtctgtg tacgcaccgg ccccgcgcgc gccggtgagc gccggcttcg
     3241 tggcgtcgtc ccctccggcg cccccagcag gcgccggccc ctcgatgtcg atgcgcggca
     3301 gccactccag ccagcgcggc atccaccagt agcggtcgcc gatgagcgcg atcagcgccg
     3361 gcgcgacgat cacccgcacc acgaacgcgt cgagcagcac ggcgatggcg agcccgaagc
     3421 cgagctcctg caacacgacg aggcggccgc tgcccatgcc gaggaacacg gccgccatga
     3481 tgagcgcggc gccggtgatg atccccgccg tggactgcac gccgaacacc accgcctcgc
     3541 cgctgtcgtg cgcctcggtc cagcgctcgc ggatgcggct gagcaggaac acctggtagt
     3601 ccatgctcag cccgaagagc acgcagaaca tgagcagcgg cacccacgac tcgatcgcct
     3661 ccatccgggt caggcccacg acctcggcgc cccagccgtg ctggaacacg agcgtcagcg
     3721 cgccgtaggc ggcgcccacc gacagcaggt tcatgaggat cgccgtcgcg gccacgacca
     3781 cggagcggaa ggcgagcagc agcaggatga agctgagcag cagcacggtg gcgatcacga
     3841 tgggcatgta gccgtccgtc aggtcgaccg agtcgatctg caccgcggtg gtgccgccca
     3901 cgtagacctc ggcgccggtg ccggcgaatg actccggcgc caacgtgtcg cgcaggtcgt
     3961 ggacgacgac cttggccctc tcggacatcg cgtcggcgtc ctggatcatc tggatcaccg
     4021 ccagcgaacc gtcggcgctg gtcgagaagc cggtcacggt gaatcgcccg tcctcgtcca
     4081 ggcggtcgac gaagcgctcg atgctgccct gcaccttctc gctgcccagg tcgccgtcga
     4141 ccaccacggt ggtcggctcg ctcataccgg cggcgaagtc gctcttgagc atatcgaggc
     4201 cgcgcttgga ggtgtacgac tgtgggaacg cgtcggcgct gaagccgccg ctgcgcatcg
     4261 tcagcgccgg ggccgcgatg gccaggagca gcacggcgcc gagggcgaga ctcacggccg
     4321 ggcgccgcat gacccgtcgc gccgtcctgc cccaccagcc gtgctcgctg tgccgccgcg
     4381 cgtagcgctc cagccagggc acgcgcagcg cgtcgacctt gtcgccgagc agcgcgagga
     4441 tggccggcag cagggtgagc gcggcggcga cggcggcgaa gacggcgctc atggcgccga
     4501 cgccgaggct cacgaagatc gagagtggca cgatgagcat gccgacgagc gacagcacca
     4561 ccgtcatgcc gctgaagaac accgcgttgg acgccgtcgc cgacgcggcc ccgatcgccg
     4621 ccatctcgtc caggccgccg tgacgctcct cgcggtagcg cgagacgatg aacagcgagt
     4681 agtcgatgcc gacggcgagt cccgtcgccg tgaggatgtt gagcgcgaag atcgagagct
     4741 cgaagttctg cccgatgagc accgtgatcg ccaggcccac gacgatggag aagatgctga
     4801 gcacgagcgg gagaccggcc gctaccaggg cgccgaacac gaccaccagg atgacgagcg
     4861 ccaccgggat gccgatcgcc tcgccgcggc gcaggtcttt ctcggcgagg tcctgcgcgt
     4921 ccttcatgag cgacgccgcg cccgtcacgg ccacctcgaa ctccggctgc gcgtccgcct
     4981 cctcgaccac ggcgtacacg ccgtcgacgt ggttcacggc ctcgtccgcc gcggccgcga
     5041 gctcgacctt catgatggtc gtgtgcccgt cgtccgaggt gagcgcgtcg cgcgtctcct
     5101 cgtccagccc ctcgtcgaac acgctgagga cggtgacgac gtccgcctcc ggcaaggcgt
     5161 cgatctggtc gcggatacgg acgacctcac gcctgaacgc ggggtcgtcc acggtgagtc
     5221 cgtcgctgtg gatgacgacg acctcgctgt cgtggttgtt caaggtcccg gcggacgaca
     5281 gggcgtcgct cagctgtccg gctcccgccg acgccgagtc gaggccgcgg ctgagctcgc
     5341 tggcgccggc gcgcacggcg tccgtcccgg aagcgagctc gccggcgccg gcagcggcgc
     5401 cgctcacgcc ggaggcgacc ttgccggagc cggcggcgag ctgccgcgag ccggcggcga
     5461 gctcctggga gccggaggcc gcggagccga tgccgctcga gagggaagcg ctgcccgacg
     5521 cgagtctgcc catgctccgg tccagctgcc gggcgccgct cgcgctgcgc gcgagaccgg
     5581 cgtcgagggc gcgcgcgccg gccgcgaggc tcgacgcgcc agaggccagt ctgccggcgc
     5641 cggcggccac ggacgagacg ccggcgctcg cggactgcag gccggacgag agaccggacg
     5701 tgccggagac cagctggcct gagagtccca tgatctgctg gaacgcgggg tcgtccgcca
     5761 cctccgggtg cgcctggagg taggcggcga cgagctggtc cagcgtcgcc gccccaccgg
     5821 cgatctgctc cgcggacgcc gcggccttgc cgacgcccgc tgcggcgccg tccgcgccgc
     5881 cggccaggtc gctcgcgccg ctcgccacct tgccggcggc ggcggcgagc ctgtcgccgc
     5941 cctgcgacag ctgctgcagt cccgcgctga agctgccggt cgcgccgtcg acctcggcga
     6001 ggccagagct caggctcgcc gccccctgcg aggcgccggc caggccgttg ctcaaggact
     6061 gggcgccaga gcgcgcccgc tcggtgccgc tgctgacctc accggctccc cgcgccaact
     6121 tcctgaggcc tcgctggagc tctttcgcgc cggacgacac cgaccccgag cccctggcca
     6181 gctggcgcgc gcccttccgg gcgtcgtcga ggccgtcggc caggctctgc acagaggcgc
     6241 cggtctgggc gagcgcgctc cctccaccgc cgccgccgtg ctcctcgatg agccggaccg
     6301 ccttctcgga gtcgggttcg ttcaggaact tggaggacga cgtgaagacg tcgccccagg
     6361 tcaggtacac gccccccgcc atcacgagcg cgaccagcca caggccgatc gtcaaccagg
     6421 gatgccgggc acaggcgagc gcaagccgct gggtgaagcc atgcctttgc tcgctcacgc
     6481 cacgcccggt cgtgcggagg tcacattcgc cgaaccgcaa atcgccatcg tcattgcaaa
     6541 catgatttgc agtatgggcg accgccgcat cggtcgcaac caggacccgg ccgtcccgct
     6601 cgcccgcccg tccgcgtcct cgccgccaga cggggcggtc gccgtcaggt caggtgatgc
     6661 tcagggacgc gtcgccggtc ggcaccagcc ggtcgccgaa ccaggcggag ggatagacgt
     6721 aggccatgcc gcggatactt acgagaaccc ggccgcggaa gccagcctca tcgccgggcc
     6781 cagagcgcca gcgcgggcgc cgcccgccgg agcgccatcc aggccgtgac ggctccggac
     6841 agcatggccc acggccggcg gaactcgctc gccggggtgt cgagcacgac gcgcaggacg
     6901 gcgaacggcc tccccgccgc ggccggcgcc agccacgccg actccatgtc gaccgccagc
     6961 acgccctcgc gcgccagtac tccgcgctcc gcaccccgca ccacgtggcc gacggaggcc
     7021 agcggcccgg cgtggatgcg ctcgatcccc agggcggcga gggcctcgat gaggggcaga
     7081 ctgtcgcagc ccgtgacgcc gtccggaccg cgcacctcgc cggcgacaac cacgtctccg
     7141 gcgcgcagcc cggcaacggt ggccccgcag aacccggcga ccgctacggc gtcggccgga
     7201 gcggaggcgg cgcggcgcgc ggccgcacgg gccctggccg cgcccatacc ggagcacagc
     7261 acccgggcat ccggcagacc gcgccgcaga gccgcgcgct cgatgcgcag cggcgcgatc
     7321 acgagcaggc gggcgtggtc ggcagtcacg ccgaccacgc tataccaacg aggcggcgac
     7381 gcgatacgcg ccgccgcctc gtcctcgccc ggcgtcaggc gctgtgctcg gcttcgcgtc
     7441 ggatcctgta gtcgagcgcc aggacaccag tgccgaacct ctgcgaccgc accagcttca
     7501 cgggcacctg cgcgttccat ccgtcgaaga gacgcctccc ctcgccgacc acgaccgggc
     7561 aaaccatgag ccggagctcg tccagcaggc cgtagcgcac cagggaccgg aggagggtcg
     7621 cgctgccgtt gacaaggatg ctcctgcccg gccgtcgctt cagttcggcg atctcgacgc
     7681 ctggatcacc cttgatcaag gtggagttct gccacccgac cgactccagt gtggtcgaga
     7741 cgacgaactt gggaacgccg ttcatgaagt ccgcgaccgg cccctcggcg ttgggccagg
     7801 cctgggcgta ggtctcgtag gtccgacgcc cgagcaggag ggcgtcggcc tcggccatcg
     7861 cctgccccat ctcctgcccc atctcgttgt cgaagtaggg ggactgccat ttctcgggcg
     7921 cttccacgac gccgtccagc gacgtgaaca gcccggcgat tatcggtctc atctccatct
     7981 cctttgcagt ggtgctcgtc acgcggctcg ccgtcgccgt tcgacatcgg caggctccgc
     8041 cacccggcga gtgagaccga gccccagccg cagcgccgtg gctgacaccc aggcccacac
     8101 gatgaccgca gcgacgaagt agcggatgct tgcgccgccc tgtcccggcc acatgctgag
     8161 ggcgatggcg gtggccgcgg tcgcgagcgc gaaggcagcc cagccccggc gaccctgtgc
     8221 gacgtcgcgg cgtgcgaaca cgaggcaggc gatgcccgcc gagcccatgg cgagcatgaa
     8281 gccggctccg tgcagcatgg cgtgccagct gagctgctcc ggcgtccccc cgggcgcccc
     8341 cggagggaag cccagggacg ggtcggcgac gaagacgccg gcggcgatga ggcccactcc
     8401 gaacgcgccc agaagccaag gtccccaggt cccgccccga gagcctttca gcacccttct
     8461 ggccccgacc gcgaacgctg ccacgagcag gccggagacg acgaatgtcg cgatctgcac
     8521 ccatcccagt tccccgagac tgagcaggct gatgggctgg ctgccgaggt cataccccgc
     8581 gcgggtcagc gcctggatga gggccgcggc gacgaacagc ggccccgcga ccacgccgca
     8641 gacgagaagc cgaagcgtcc ttccgctggt ggtcgccgac agcgcaccgg acgggcgcgc
     8701 gacctgatag tcattcatgg gtcttccttc ctcacgcgga ccacttgccc gccgatgtag
     8761 actagacggt accggtccac aggaaaaatg gaactaaact gtccagtaca gttccgagca
     8821 aggagggtga gcgtggcagg accacctcct accgccgagg aacctcgaga aggacccgag
     8881 cggccgccgg aaggccgttc ggcacgcaag caccgcgcca tcctcagcgc cgccggtgag
     8941 ctgttcctcc gtcagggcta tctggggacc agtgtcgacg agatcgccgc ttcggccgga
     9001 gtgtccaagc agaccgtcta caagcacttc gacgacaagc agcacttgtt caccgccgtc
     9061 gtcttcgcca gcatcgatca cgtgggccgg ccgttctacg aggagatcca tcgccttgaa
     9121 gacagcgacg acctcgaggc agacctgcgg gagctcgcga agcggctctc ggccgtcgca
     9181 acagaccagc gcctcctcca ggtgaggcgc ctcgtgatcg gtgaggcggc gcgcttcccg
     9241 gagcttggcc acgcttacta cgagcgcgcc gtcgaccgta ccacccggag cctggaggcg
     9301 aggttccgcg gccttgcgga gcgaggcctt ctgcgactgg acgatcccta tctcgccgca
     9361 cagacgttca tctggctggt cctgtcggtc ccgctcaaca gagccatgtt ctccccggag
     9421 accgagacgg atgccgctgg gctccgaagg atcgccgacg aggccgtgcg catcttcctc
     9481 gccgcctacg gcgccacgcc ggcgtcacct cgccgcggcg cacggccgcc tgctcaagcc
     9541 tagctctcgg gccgctgatc cggagaagcc tggtccgggc cactcgcgag accgccgccg
     9601 gcgcggcgca gcgcctggga gccgaagcgc gcccgcacgc ggtcgacggc ctcatcgcac
     9661 gcgtggtcac gcgcggagtc gtcgagcgcg gtctggcggg cctcggtgag gccgcccagg
     9721 gtgaggccga cgaggcgcag gagacggccc ggctggtgca gttcggcaaa gagggtcaga
     9781 gcggcctcgt agatcacctg cgtcgtgttg gtcggagcgg gcagggtgcg ccggcggctg
     9841 accgtgtgga agcgggcgtc gcgcagcttg aggaccacgg tgcggccggc gtagccctgc
     9901 cgccgcagat cgctcgccac ctggtcggcc tgctcgagga gtgtcgcccg caggaactct
     9961 gggtcggcgg tgtcctcggc gaaggtcacc tcatgaccca tggacttggg cgccgcgtgg
    10021 ccggcgcgca ccggggcgtc gtcgccgccg aaggcaagct ccctgagcga gccggccgtc
    10081 ttcgcgaatg acgcctcgag caggggaagc ggcgcgtcct gcaggtcccc gatgctcgtg
    10141 atccccaggg agctcaggcg cttgaccgtc accgggccga tgcccgagac ggcgccgacc
    10201 ggcatggagc gcaggcggcc gtgcacgtcg tcccgggtga gggtggtgag gccgccgggt
    10261 ttgtccagct cggcggccag cttcgcgagc agccgcgtgg gtccgatgcc gatcgagcag
    10321 gagaggccga gctcctcgcg cgtcagccgc tggatctggc cggctatctc ttgcggagag
    10381 ccgaacagcc gccggctgcc ggtcacgtcg aggaaggcct cgtcgatcga caccggctcg
    10441 acgaggtcgg tgaagcgccc gtagatggcc agcagtcgct gctggaccgc cttgtaggcg
    10501 gccatgtcca cgggcaggaa gacgccctgc gggcagcgcc tcagggcctg cgccatcggc
    10561 atcgccgtgc gcacgccgca ggcgcgcgcc tcgtaggagg cggtgctgac gacgccgcgg
    10621 ctctcggctc ggccgccgac gatgaccggc cggccgcgca gtccgggccg gcgagcctgc
    10681 tccaccgcag cgaagaaggc gtccatgtcg atcagcagga tggcacgggg catggcctca
    10741 tcctgctggg caaaggcgcc atggttcaag tctgctgccg ctgcagccac ctcgccgctg
    10801 agacgccggc cctcgcgccg tcgccggcgg cgatgatgat ctgcttgccg ggcgcgtccg
    10861 tcacgtcacc ggcggcgaag atcccggggc ggctcgtcat gaggtcgcgg ccgacctcaa
    10921 cctctcccgc ggcgttgacc cgcacgaggc cgccggtgaa ctccccgacg gggatcgtgc
    10981 cggcctcgat gaacaccgcg tccacgggca ggaccagttc gttctccgcg tcgtcctgga
    11041 ccaccagagc ggtgacgtgg tcttcgccct cgatcctcct ggcctgcagg cccgtgcgca
    11101 tctccacctg agggtcggcc tcgagcttgc tgagcagcgt ctggggagcg cgcagctcct
    11161 gctcgctgac caggaccacg tccgcgtcga gggcggcgag ctgcagcgcg gcctcggccg
    11221 ctgactcgcc cgggccgacc acggcgaccg ggcggccgcg gaagaacgcc gcgtcgcagt
    11281 aggcgcagta gctgacgccg cgacccacca gttccctctc gcccgggacg gacaggcgca
    11341 ccggggcgcg tccgctggcg atgatcaccg cgcgcccgcg cagctgcagc ccctcgcgcg
    11401 aatggacctc gaagagcgcg tccgacggaa cgaccgcgtc ggcataccgg ccctcgaagg
    11461 tgtcgatggc gaagccggcc gcgtgcgcac ggaagcgatc ggccagctcg ctcccggcga
    11521 tgagctcgaa cccgaggtag ttctccacgt ggctcgccca ctgcgcctgc ccgcccagct
    11581 cgcccgcgat gatggccgtg gacaggccct ggcgcgaggt gtagatggcg gccgccagcc
    11641 cggcggggcc gccaccgatg acgaccacgt cgtagcggtc cttcggctcc tgctcagcca
    11701 cctgtcacct cgcggttcgt gcttgggtcg gccaggagca ccatgcccgc ttcggcgccg
    11761 ctcctcacca ccgcgcatgg ccggccggga cgagcagagg gcgggaagag cagacctcgc
    11821 tcttcccgcc ctcgttcacc tgcgagagca gcggctcagt cgagccgtca gatgctgctc
    11881 aatcgagccc actgtagatg gcattggcga ccaggcggaa cgaatcctcc gggtggccgc
    11941 ggaagatggg gtccagcccg atgagcgccg catcctgcgt gcccgacgcc caaccgatga
    12001 cgaccggctt gccggctgcc ccgctggtct gccagtccgg ccagtagccg gagacgagga
    12061 agtcgggatc gtggatcgtc gcggagacgc ggacatccgc cgcgagcccg ctgaacgtcc
    12121 tcgtcccgtt ggcgaagccg tacccctgcg ccccgaatcc gctgcccacc gggtcgttga
    12181 cgttgtagtc gacgcggatg gcggagtcga gtgggccgcc agtgtagctg gtggccgtga
    12241 agacgaagga cggcagcatc tgggtggcca gaagggcgcc tgtgcctccg atgccgatgt
    12301 agtccttgcc ggccgcgaag tagtcggtca gcctggcctt gccggtggcg ttgagccccc
    12361 cggtattgaa gtggacgctg gtgttgatga acacgtcgta ctccgtcagc ggcggcttgg
    12421 tcgcgtcgtt cagtgtgctg accgtgatca cgtcgtacgc gaagccaagt cgcttgagca
    12481 actccctccc tccggcatcc gagaaggcga cggcgatgcg ctgccggcgc agcgcgatcg
    12541 cgcctgcagg cacgccgcga agagcgtacg cgtcgaggcc ataccggctg gccagctcat
    12601 tggcgaccgt cttcgcggtg ccgggcagga tgaaggcgcc ggcgtcgaag gcgcgtccgc
    12661 cgtcggtgaa ggaagcaggc gcgcgtgaca cggcgactcc gcgcgccagc aggtcgttcg
    12721 tggcctggat ggcggcgatg ctggtcggga ggtaggcgta ggcgccggcc ttgccgccct
    12781 gcaccgtacc ttgcggtgcg tcagccttgg cgatcgctcg cgtctggagg gccatcgggt
    12841 cctccatcac cgcgcgacgc acgccccaca gcaacgggtg cgaccacacc gtgggcggcg
    12901 agtagaactc cagcccgggg atggccgaga ggtcgaggcc gtccgacagg atggtgttgg
    12961 cgaggcctcg cttcggctgg tccagccaca cgacgtaggt cccggccggg taggccacgc
    13021 cgtccagagt gaagcccttg ctggccttct cgacctggac gtcgttgaag agcaggaagt
    13081 ccaccagccg ggcgggcgcc tgcgggctga tctggaacgg ctcgtcggcc gggatcacgt
    13141 aggccgccgg gaagccgttg gggatgacgt cctggtactg cggccactgc ggcaacagtt
    13201 ccggcgggat gggctgctgc ggcagcgaga ggaagccgcg ccggaagatc tcgacctggt
    13261 cgcggaccat gcccacgcgg ttctcggcga cgaagtcgag cgcgccccac accgccgcat
    13321 agtgggcgtc cacgccgcgc gcgtcgcggt acggcgtctc catggtgtgg ccgtaggcgc
    13381 cgtggaacat ggcgtacatc ggcgcgtagg tcgggggcca gtcgtcccac tccagcggat
    13441 cgtcgcggta ggggatctgc gcgctgaagc cggtctgggc gaacagctcg gcctccatgg
    13501 cctcggcctg ggcgagcgcc cacttgatgt acaggtcgta ctcgtagttc gggttgtgcg
    13561 gcggcgtaca cggctcgatc agcatcggat tgacgaaacc gtgcaggtcg aggacgacca
    13621 tcggattcca ctccgtgaac accccgaccg tcgcccgcgt ctcgggctgc gactgggtga
    13681 tgaagtcgcg gttgatgtcg atggcgttgg cgttctgcct cgtgccgagc acgcgaccgt
    13741 ccgggttctg gcacacgttg atgagcagga tctcgttctc gaggacatcc aggacttcgg
    13801 gctcgttgct gaaggcgaac tcctcgatca gcctcatggc ggcatcggtg ccggggtact
    13861 cgttgccgtg gatgctgccg ttgatgaaca cggggacctt gaagtcaccg aacctgtcga
    13921 tgagctcctg ggcgtgctcc ggatcccgga tcatcgcctg gcggatggcc tggtactgcc
    13981 cgaggcggcc ctgcgcctgg gggtcggaga tggtgaccag gaacaggttc cggcccccgg
    14041 ccgactgccc gatgacctcc accttgacgc ggttgctggc catctcgatc tgacggagcc
    14101 tgggagcgat ctcggagtag aggatcgagt cgtactgtgg tgacgggact tcggcctccg
    14161 gagtcacgtc ccaccagttg cccaccgtgg atggctcggc ggccgcggcc acgccggcga
    14221 acgccagcag cgcgaccgcc gccagaagca ggaagagcaa aggagtgctg cgagataggg
    14281 agcgtctcat tcgggaacct ccacgtcgct gacggagtcg ttggcgcact cgtgcgtcac
    14341 ctcctcttcc ctgcccggtc agggcgcgcc atgcctcttg cgggcagctc gcgtgcatgg
    14401 agttcgagcc taccaccaag ccgcgcggcg gccggcgttg acgacgctga gggtcaccga
    14461 gtgacggacg cgcactgact acagcagcga caccgggatc cgcacttcgt tcaggtgcga
    14521 gccgtccttg gccgacgcct cgtagaccac gagccagccg ctgtcgctgc cggtcgagta
    14581 cacgatcttc tttctgaacg cgccgcggcc gggggcgcct tcggtggccg tcgtgaacgt
    14641 ctcgacgagc gtctgctcgc cggcgtcgag caggtcgatg ttgaccgtgg cctcgaagac
    14701 ggtggcgcga ccctggacga tgaaggggct cgacagggtc gcgccgacgc ccggccgctc
    14761 gacgaagatg gccggctcga agctctgcca gtcggcgcgg cgctgcgggg tctccacgat
    14821 cacgccctcc ccgccgatcg tcgcgacgcg ctcgccgtcg agcatgaact cgacggctct
    14881 gatggtcttg aactgcgtca tcgtgtacac gacctgcgcg acgcgcgcgg tcatcgagag
    14941 cgagccgccg cccgaggcga actcgcgcga caggtcgacg gtcgcgacgg cgccgtcggc
    15001 ggagatgccg agcagcctgg tgcccttggg gacggcggta ccgaggccgg cggccttctc
    15061 gtcgccgttc gggcctgcga cgagggcctc catcgcggct ctggcgaccg cgaccgtctt
    15121 cggcagcgta cgctcggccg tgccgagctc ttcaccgcgc aggaagtaga cgagcacggt
    15181 cgtctcgccg gcggccgtct cgggcggcga cgggctcgcc gccggggtct cggtcgccgc
    15241 tggggtctcg gtcgccggct gggtcgccgc cggggtgccg tcggagacgg gcggagtctc
    15301 ctccgtaccg cacgcggcga gcaccagcgg cgccgcgagc gcgagcagcg ccaggacggc
    15361 gagcgccatc cagggcgaga ccgcgccgcg ccgcggggag cgcctgtcgg gtcggtgggc
    15421 gggtgtcaaa gcggggcgcg acatgaggat cgcctccaac tggctgcgac ggcgggcaga
    15481 cggcgcctgc cgcctgcccc catgctgacg tgacgcaaca agagacgcct gcggcggcgg
    15541 aaacgttccg tcggccgccg aagcatcgat cagccgcccg ccgagccctc ccggatgcac
    15601 gcgaccggct cgggggccgg ccggccggcc cccgcggcgc cggccgagcg ctcagcgagc
    15661 gcccccacgt aggcccggtt cgtcgggtcc ttctgccagc ggtggatata cagcctgccc
    15721 cagaggcgct ccagcgaggt gccgaggcgc aggcgcgcga gaggtctgac gatgttgccc
    15781 accgagtaga accgccgata gccgtcgatg accccggtct gcagctggcc ggcggtcatg
    15841 ttctgcggct cgaagatgac gtgctggccg tcgtagaact gccagcggcg ctcgaagacg
    15901 cgttcgtcgc cgttcatcat ctcgtactgc ctggtgccga tccctggtgt gaggatgttg
    15961 agcatcaggc tgtcgatgcg gtagcgctcg gcgaaggcga cggtagcgcc ggccgtccgc
    16021 acggtgtcgc tgtcggcgcc cacgacgaac atgccgtgga cgtggatccc gtggcggtgc
    16081 aaggcggcga tcgcgttcgt gatgtcctcg accgtctgcg acttcgcgta gctgtccagc
    16141 gtcgcctggt cgaccgattc gaacccgagg gccaggcgat gacagccgga gcgctccatg
    16201 agcgtcagca actcctcgtc gcgggccacg tcggtgcgca cctgggcctg ccacttcagg
    16261 gtgatgccgc ggtcgatcat cagctgcagg atcttcttga gccgccgctt gtccgcggcg
    16321 aagttgtcgt cgtagaagaa gaccgagcgc ggcttcttcg cctccagttc cgcgatgacg
    16381 ctctccgggc tgcgcatgcg gtacttgcgg ccgaacatca tcgtcacggt gcagaaggtg
    16441 cagtcgaacg ggcaccccag gctggtcatg atcggcgtct cccgcatgcg ctcggagccc
    16501 acgatcaagg agaggtccgg catcggcagc gagtcgagat cggcgcagcg ctcgcgcagc
    16561 tcgttgtgca cggggagccc gtcgcgccag aaggacagcc cgttgacgga gtcgagctca
    16621 cgctcgccgc ggagcgcctc gaggagctcc agcatgagac cgtcgccgcc ctccccgcgc
    16681 gcgacgtagt cggcgtgcgg catggcgtcg tcgggcacga agctcgggtg ggggccgccg
    16741 atgatcacgg ggagcccggc ggcccgcagc tcatccgcca gcgcgtaggc ggtcggcgca
    16801 gtcgaggtgg tcgtcgagat gccgacgagg tcggccgagg cgacgtcggc gcggtcgatg
    16861 ggcgccgcct ggggggcgta gatgagtacg tcgtggccgg cggccttgag cgccgcgccg
    16921 atcaggggca ggccgagacg cggatagaag ccgtaggaga ggatgttgat gctgggcggg
    16981 gcaggttcga tgaggcggat cttcaaggcg cttcccttct cggcccgggc ctgccgcacg
    17041 cagcccgtgt acgtgcatgt tcccccggcc ctcacggcga cgcaagtcga cagtgagcct
    17101 gcgttacccc tctcccctgt aagtagtccg ttaagcgaga agaccgggcg gctctgagag
    17161 ccgcccggtc ttcaggtgga gctacgacgt tgccgtctct gttacgacag cacccgcacg
    17221 ttggtcgcct gcggcttgtt gttcttgccg gtgccctgct cgaaggagac ggcctgaccc
    17281 tcatcgaggg acttgaagcc ctcgctctgg atctccgaga agtgaacgaa gaggtcgtcg
    17341 ccgccctcgt gctcgatgaa gccatagccc ttgtcggcgt tgaaccactt tactttgcct
    17401 gttgccatac tgatcaaatc ccttcgccca ctcggggcaa aacaaaaacg acgtgatttc
    17461 cgcggagaaa tgcggtgtgt cacgtcgcaa tcctgcgggc ccactcggcc cgtcccgaag
    17521 agtatagcag attccgcggc gccgtgctca cggcgcgggt ggagcgcttt cagggccctc
    17581 ggccgctggc tttgctacga aattaggcgt agaatgggat ggtctacgga agggtccacc
    17641 catggactgt cgctccaccg ttctgcccca ctcgtgaagt cccgcgtcgc cggcgggttc
    17701 atcctcccga gcaccgggaa gctcagcgac cggatcaagt cgtacgggag cgggcgcatc
    17761 tgcgaggcgc ggggctgcac caccatcctc tcggcctaca accctgcgga attctgctcg
    17821 acgcacaaac gcgccggtac acggtagccc cggtctcggt ccggccgccc gctgccccgt
    17881 gccaggagcg catcgcggcc cgtcctcgat cctgacgtac cccctagcgg cgatcgccgc
    17941 tgcggccgac cgtgaggggc agcagcgccg ggcgaggcgc gcttcgagcg tgccggcgag
    18001 gaggccatcc gggcgggcgc ggtggcggcc tcaggtcgtc gaatcccacc cgcgatgagg
    18061 acgacgaccc ggagcgcatc gccgagcccg ccgccagcgc acccgccaca gtcgcaagca
    18121 gggcgatgcc ggcagcctgt tgggcatcgc cggcaagcgg ctcgctgtcg agcggcccgc
    18181 tgtcgagcgg cccgctgggg aacggctcgc aaccccagta ctgctgcgcg aggccgcgcg
    18241 gccagtcgag gccgagatac tccatctgca gggtcgagaa ggaggccgcg cgtcgtgacg
    18301 ctgagcggcg ggcatggggg cgcgtcgtga gtgcgaacag ttgcccaccg caggcgacga
    18361 agcgctcgcc gggcgcggcg atgcagcgtg catgatcatc atcgggaaga tcgtgggggt
    18421 cgttctccca gacccacgtg agcgggacct tcacctcgac gctcctcgcc tcggcctttg
    18481 gggccagcgt gccgtcgtgt gaagccgcgt tggtctgact tttttagtat accacgcgcg
    18541 gggctcgtca cccgcggggc ggctgatagc gggcaagggc gaagtcgacg aaccgcgact
    18601 gcacctgctc gctcgcgagc agcttcgcgg ccggcggcag cgacaggatg gctccgagca
    18661 ccgcgttggc gaacgccggg cctcggcccg ccgtgccgtc cacgagcaag tccgccagcc
    18721 tcccccgctc gagcatccgc cgggtgaggt actcgacggc gttgagcggg acgtggggcg
    18781 ctcgcgggcg ccggaccatg gtcacggcgt cctcacggca cgccccgacg cagacgccgc
    18841 agccgatgca gcgggcgagg tcgacgcggc tggtcaatgg cgtcgcggca tcgtctccgt
    18901 cgttctgccg agcgccctga cgggggacga gcgagagagc ctggaccggg caggcccgca
    18961 cgcacctgcc gcagcccgtg caacggtgat cgtcaacggc gggctggaag gcgctcggct
    19021 gcatgaccgc catgttgttc tgagccgagc gcagctcgac gcagcaacag gagcagcagc
    19081 tgcagatgta gctcagctcc cgctgcacgt tgtccgcgat gtgcacgagg ccgtgctcgc
    19141 gagcggcctc gagaagctcg cgtccctcct ccctgccgat ctgccgggcg tggccgtgcc
    19201 ggatcaggta gcgcgccgcc acccccaggc tcatgcacgt ctccagggga tagtcgcagg
    19261 ccgtgccgag atgcgttgcg gtgtggcggc agaagcagtt cgtgacggcc aggcagtcgg
    19321 ccctctccac atacga
//
LOCUS       DASUMX010000146         3680 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_476443, whole genome shotgun sequence.
ACCESSION   DASUMX010000146 DASUMX010000000
VERSION     DASUMX010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3680
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_476443"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..132)
                     /locus_tag="VFZ86_07105"
     CDS             complement(<1..132)
                     /locus_tag="VFZ86_07105"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX5642096.1"
                     /translation="MLVELNAEQLAAAQHDDGPLLIVAGAGTGKTAALVHRVAHLIEC
                     "
     gene            complement(232..705)
                     /locus_tag="VFZ86_07110"
     CDS             complement(232..705)
                     /locus_tag="VFZ86_07110"
                     /inference="COORDINATES: protein motif:HMM:NF039183.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5658 family protein"
                     /protein_id="HEX5642097.1"
                     /translation="MEIAAEQAPGALRARGPDRRRERRFIFLDRRSGFDRRRREGRSP
                     VSSAVEARLLQLRDQPRVLAETLILVNLLSVLDLLLTLSVLRLGAIELNPIMDWLFEL
                     GPGPAATAKIGVMALATFGLWSLRRYRKALATTVLLLTGYGALVTFEIIGLIGLL"
     gene            826..2238
                     /locus_tag="VFZ86_07115"
     CDS             826..2238
                     /locus_tag="VFZ86_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020579865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX5642098.1"
                     /translation="MTASGQTDDVVRALRAHLTGEVVGAGDEGYDTARSVWNGMIDKR
                     PAAVARCATSDDVSAAVGLARAAGLPIAVRGGGHNVAGLATSEGGVVLDLSRMNAVTV
                     DAERRRVTVQGGAIWREVDAATQAYGLAAPAGLVSETGVAGLTLGGGIGWLRRKYGLT
                     CDNLVAAELVTAQGELVRASAEENPDLLWALRGGGGNFGVVTSFEFLLHPVGPEVAYA
                     LVFYDGAETVAGLRALRAVAGDMPDEVAPIAFTGSVPEMEGIPAEAAGRPMLAVAAAY
                     VGPPDEGERQLRPLQGLATPIADLSGRQPYAQLQQFLDEDYPRGRHYYWKSAALAELS
                     DAVIDVVAEYASTQPSPLSTIDVWQMGGALREEPAGGSAYAGRSAGWLVNPEADWDEA
                     DADDVNVAWARSLIKALQPHTVGTYLNFPGFLEEGELQLRAGFGDHYERLVEVKTRWD
                     PENVFRLNHNIPPRRLGRAA"
     gene            2310..>3680
                     /locus_tag="VFZ86_07120"
     CDS             2310..>3680
                     /locus_tag="VFZ86_07120"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX5642099.1"
                     /translation="MDTEREGRRLRGRRRWLRVAAAAEMLEVSEHTLRRWADAGVIPC
                     RRTPSGQRQFLPGDLQRFLERCGAGGNGHADGGALAAVSRAAARTEDPMEALAQVARA
                     LAGEPGVDRCLVMEHDGALDALVGLASAGATPAAADDAPPAEDPAAGEVWLLVERPAE
                     REMLRGGEPLRGPSRLCIPFAIGATANGCLVLQGPGAGRLSPGRLEHARDLGDLAALA
                     VHRLQAARDRAAQAARMESLLHAGRSMTSSLVLQDVLDAVAREVVDTFAARYCVIWEY
                     AEDQDALIERAGFGVDPGFCVDGETVMLEERSKEREILFGPEPVLETVSDPELDPQSR
                     VSMERWGEKTCLSLPLQFGGATLGLLAICETERERRFTGEEMELARGLANQASAAVHN
                     ARMYRDLEQRNEELVARARRERLLNELSLKLGSSLDLREVLDSACRRICSILDAGGCE
                     IWARREA"
BASE COUNT          513 a         1278 c         1416 g          473 t
ORIGIN      
        1 gcactcgatg aggtgggcga cgcggtgcac gagcgcggcc gtcttgccgg tgccggcgcc
       61 ggcgacgatg agcagcgggc cgtcgtcgtg ctgggcggcg gccagctgct cggcgttgag
      121 ctcgacgagc atgaggagtc ctcctggggt gcggtgggcg tgaggctagc accctggtcg
      181 gacggatgcg aacggatgtt cgagtgaccg cgcccgaggg acgaacgcgc gttacagcag
      241 cccgatgagc ccgatgatct cgaacgtcac cagcgcgccg tagccggtga ggaggagcac
      301 cgtggtcgcg agcgccttgc ggtagcggcg cagggaccac agcccgaacg tggcgagcgc
      361 catgacgccg atcttggccg tcgccgcagg gcccggtcca agctcgaaca gccagtccat
      421 gatcgggttg agctcgatgg ctcccagacg cagcaccgac agcgtgagga gcaggtcgag
      481 cacgctgagg aggttgacca ggatgagcgt ctccgccagt acgcgcggct ggtcgcggag
      541 ctgcagcagc cgcgcctcga cggcggacga caccggcgag cggccttcgc ggcggcgccg
      601 gtcgaagccc gagcgccggt cgaggaagat gaaccggcgc tcccggcgcc ggtctggccc
      661 gcgggcccgg agcgccccgg gcgcctgttc ggcggcgatc tccatgcggc gagcataccc
      721 cgcacggccg gggatgaggc catgcccttc gccgacggcc ggcctgcgcg gcgaccgcga
      781 cgcccgggaa cacccaggcc atcggcactt cacgaggagg tcgacatgac cgcttccggt
      841 cagacagatg acgtcgtgcg tgcgctgagg gcccacctga cgggtgaggt ggtaggcgcc
      901 ggcgacgagg gatacgacac ggcccgttcc gtgtggaacg ggatgatcga caagcgtccg
      961 gccgcggtcg ctcgctgcgc gaccagcgac gatgtgtccg cggccgtcgg cctcgcccgc
     1021 gcggccggac tgcccatcgc ggtgcggggt ggtgggcaca acgtcgccgg gctcgccacc
     1081 agcgagggcg gcgtggtgct cgacctgtca cgcatgaacg cggtgacggt cgacgctgag
     1141 cgccgccgcg tgaccgtcca gggcggcgcc atctggcgcg aggtggacgc cgcgacccag
     1201 gcctacgggc tcgcggctcc ggccggcctg gtctccgaga cgggtgtggc cggcctcacg
     1261 ctcggcggcg gcatcggctg gctgcggcgc aagtacggcc tcacctgcga caacctggtc
     1321 gcggccgagc tggtgaccgc gcagggagag ctggtccggg cgtccgccga ggagaacccg
     1381 gacctgctct gggccctgcg cggcggcggc ggcaacttcg gcgtcgtcac gtcgttcgag
     1441 ttcctcctgc accccgtcgg ccccgaggtc gcctacgcgc tcgtgttcta cgacggcgcg
     1501 gagaccgtcg cggggctccg cgcgctgcgc gcggtcgccg gcgacatgcc ggacgaggtc
     1561 gctcccatcg ccttcaccgg cagcgtcccc gagatggagg gcatcccggc cgaagcggcc
     1621 gggcgcccga tgctcgcggt ggcggcggcc tacgtgggcc cgcccgacga gggcgagcgt
     1681 cagctgcggc cgctgcaggg tctcgcgacg cccatcgccg acctcagcgg ccgccagccc
     1741 tacgcgcagc tgcagcagtt cctcgacgag gactacccgc gcgggcggca ctactactgg
     1801 aagtcggcgg ccctggccga gctcagcgac gccgtgatcg acgtcgtcgc cgagtacgcg
     1861 tccacgcagc cgtcgccgct cagcaccatc gacgtgtggc agatgggcgg ggcgctgcgc
     1921 gaggagccgg cgggaggctc cgcctatgcc ggtcgctccg ccggctggct ggtcaacccc
     1981 gaggcggact gggacgaagc ggacgccgac gacgtcaacg tcgcgtgggc gcgcagcctc
     2041 atcaaggccc tgcagccgca caccgtgggc acctatctca acttccccgg gttcctcgag
     2101 gagggcgagc tgcagctgcg cgccgggttc ggcgaccact acgagcgtct ggtcgaggtg
     2161 aagacgcgct gggacccgga gaacgtgttc cggctcaacc acaacatccc gccgcggcgg
     2221 ctcggcaggg cggcgtgacg ggcggcacat ggcggcgcct gcgcgaagag atggtacaga
     2281 aacgagggtg aacggccgac aagggccgta tggacacgga gagagaggga cggcggctgc
     2341 gcggccgccg tcgctggctg cgcgtggcgg ctgcggccga gatgctggag gtcagtgagc
     2401 acacgctgcg tcgctgggcg gacgcagggg tgatcccatg tcgccgcacg cccagcggtc
     2461 agcgtcagtt cctgcccggc gacctgcagc ggttcctcga gagatgcggg gccggcggca
     2521 acgggcatgc ggacggcggc gccctcgccg cggtgagccg tgccgcggct cggaccgagg
     2581 atcccatgga ggcgctggcg caggtggcgc gcgcgctggc gggtgagccc ggcgtggacc
     2641 gctgcctcgt catggagcac gacggtgcgc tcgacgccct ggtaggcctc gcgagcgcgg
     2701 gcgcgacacc ggccgccgcc gatgacgccc ctccagctga agatccggcg gccggggagg
     2761 tctggttgct cgtcgagcga ccggccgagc gcgagatgct gcgcggcggg gagccgctgc
     2821 gcggaccctc gcgcctctgc atccccttcg cgatcggagc cacggcgaac ggctgcctcg
     2881 tcctccaggg acccggcgcc gggcgcctgt cgccggggcg tctcgagcac gctcgcgacc
     2941 tcggcgacct ggccgcgctc gccgtccatc gcctccaggc ggcgcgcgac cgggcggcgc
     3001 aggccgcgcg catggagtcg ctgctgcacg ccggtcgcag catgacctcg agcctggtcc
     3061 tgcaggacgt gctcgacgcc gtggcgcgcg aggtggtgga caccttcgcc gcgcgctact
     3121 gcgtgatctg ggagtacgcg gaggaccagg acgccctgat cgagcgcgcc gggttcgggg
     3181 tcgacccggg gttctgcgtg gacggcgaga cggtgatgct ggaggagcgg tccaaggagc
     3241 gcgagatcct gttcggcccg gagccggtgc tggagacggt ctcggacccg gaactcgacc
     3301 cgcagagccg ggtgtccatg gagcggtggg gcgagaagac ctgtctgtcg ctgccgctgc
     3361 agttcggcgg cgcgacgctc ggcctcctgg cgatctgcga gaccgagcgg gagcgccgct
     3421 tcaccggcga ggagatggag ctggcccgcg gcctggccaa ccaggcctcc gccgcggtgc
     3481 acaacgcccg catgtaccgc gatctcgagc agcggaacga agagctcgtc gcgcgggcac
     3541 gccgcgagcg gctgctcaac gagctcagtc tgaagctcgg ctccagcctc gacctgcggg
     3601 aggtgctcga ctccgcctgc cggcgcatct gctccatcct cgacgccggc ggctgcgaga
     3661 tctgggccag gcgcgaagcc
//
LOCUS       DASUMX010000147         2258 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_188499, whole genome shotgun sequence.
ACCESSION   DASUMX010000147 DASUMX010000000
VERSION     DASUMX010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188499"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="VFZ86_07125"
     CDS             <1..541
                     /locus_tag="VFZ86_07125"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX5642100.1"
                     /translation="TFSFPVLNAAALDIWGAEVELLWVPVDGLTLQGQLGYLNADYAE
                     FTDTIRGAGGVPVERDRSDDEPPFAPEWTARVGVSYEFGLDDYGWLTLSTDVNYRTKS
                     WLSVDNREVLTQDDFALMNVLASWQSQSRTWRASAGIRNLTDEVYKTDAQEFSSVGNI
                     QTAYYGDPRTWQLTLGYSF"
     gene            623..2200
                     /locus_tag="VFZ86_07130"
     CDS             623..2200
                     /locus_tag="VFZ86_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020399202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5642101.1"
                     /translation="MNWFEVQAPLLPEIIALHGTWLGDKPAVVDGERVFSWREFDART
                     NRVAHGLQQLGLRRGERVLVVMHNSAEMLEAIWGILKAGGVVVPLNLSIPDDAVVAMA
                     ADSEAVAAVASGDQCARLEALRPRLGGVRAGAFVAHEAALPGWHVFGVWRDAQPAQAP
                     EPGLGDDDECNIIYSSGTTGLPKGIVHTHRRRLDWAYDLALALRYHGGARTLCSLGLY
                     SNISWVGFLCTMLAGGTVVVLRAFEPRALLETVQRERITHGAMVPVQFQRLLERPDFD
                     RYDLGSLRSLMCCGSPLAPALKAEAIRRLGCELIELYGLTEGVITTLAPEDVERKLAS
                     VGKPLPGTDLRILGSEDQEVGPGESGEIVGRGRILMAGYHARPDANAEATWTDPQGRR
                     WLRTGDVGRLDEEGFLYIVDRKKDLILSGGQNIYPADIESAALQHPDVAEVAVIGVAN
                     DRWGESPLAVVVPRAGVEPDAAGLTAWINERVGRQQRVTGVVLRDSLPRNPNGKILKR
                     ELRAEYAAGRASQRSTS"
BASE COUNT          325 a          793 c          808 g          332 t
ORIGIN      
        1 gacgttcagc ttcccggttc tcaacgccgc cgcgctcgac atctggggcg ccgaggtcga
       61 gctgctgtgg gtgccggtcg acggcctgac cctgcagggc caactcggct atctcaacgc
      121 cgactacgcg gagttcaccg acacgatccg gggggcgggc ggtgtgcccg tcgagcgcga
      181 ccgttccgac gacgagccgc ccttcgcccc ggagtggacg gcgcgcgtgg gcgtgagcta
      241 cgagttcggg ctcgacgact acggctggct cacgctctcg accgacgtga actaccggac
      301 caagtcgtgg ctgtcggtgg acaaccgcga ggtgctgacg caggacgact tcgccctcat
      361 gaacgtgctc gcgagctggc agtcgcagtc gaggacctgg cgcgcctcgg ccggcatccg
      421 caacctgacg gacgaggtct acaagaccga cgcgcaggag ttctcgagcg tgggcaacat
      481 ccagaccgcg tactacggcg acccgcggac ctggcagctg acgctcggct attcgttcta
      541 gccgtcccgc tgcgaagcgc ggtggtcgcg gcctcggtgc cgcggcgccg tgcttcgcgg
      601 cgatctcaag caggaagctc tcatgaactg gttcgaggtg caggcgccgc tcctcccgga
      661 aatcatcgcc ctgcacggca cgtggctcgg cgacaagccc gccgtcgtcg acggcgagcg
      721 ggtgttctcc tggcgcgagt tcgatgcgcg gaccaaccgc gtggcccacg gcctgcagca
      781 gctcgggctg cgccgcggcg agcgcgtcct cgtggtgatg cacaacagcg ccgagatgct
      841 cgaggcgatc tggggcatcc tgaaggctgg tggcgtcgtc gtgccgctca acctgtcgat
      901 cccggacgac gccgtcgtgg cgatggccgc cgactccgaa gcggtggcgg ccgtggcctc
      961 gggcgaccag tgcgcccgtc tcgaggcgct gcgtccgcgc ctcggcggcg tgcgtgccgg
     1021 cgccttcgtc gcgcacgagg ccgcgctccc gggctggcac gtgttcggtg tgtggcgtga
     1081 cgcccagcct gcacaggcgc cggagcccgg gctcggcgac gacgacgagt gcaacatcat
     1141 ctacagctcg ggcaccacgg gcctgccgaa gggcatcgtg cacacccacc gccggcggct
     1201 cgactgggcg tacgacctcg ccctcgcgct gcgctaccac ggcggcgccc gtacgctgtg
     1261 ctcgcttggg ctgtactcga acatcagctg ggtgggattt ctctgcacca tgctggccgg
     1321 cggcaccgtc gtggtgctgc gcgccttcga gccgcgcgcg ctgctcgaga cggtccagcg
     1381 cgagcgcatc acccacggcg ccatggtgcc ggtgcagttc cagcgcctcc tcgagaggcc
     1441 cgacttcgac cgctacgacc tgggctcgct gcgcagcctg atgtgctgcg gctcgccgct
     1501 ggcgcctgcg ctgaaggccg aggcgatccg gcgcctcggc tgcgagctga tcgagctgta
     1561 cgggctcacc gagggggtga tcacgacgtt ggcccccgag gacgtcgagc gcaagctcgc
     1621 atcggtcggc aagccgctgc cgggcaccga cctgcgcatc ctcgggtcgg aagaccagga
     1681 ggtcgggccg ggggagtccg gcgagatcgt cgggcggggg cgcatcctca tggccggcta
     1741 ccacgcccgg cccgatgcca acgccgaggc cacgtggacc gacccgcagg gtcgccgctg
     1801 gctgcgcacc ggcgacgtcg gccgcctcga cgaggagggc ttcctctaca tcgtggaccg
     1861 caagaaggac ctgatcctct cgggcgggca gaacatctat ccggccgaca tcgagagcgc
     1921 cgcgctgcag caccccgacg tggcggaggt ggccgtgatc ggcgtggcga acgatcgctg
     1981 gggcgagtcg ccgctcgccg tggtcgtgcc gcgcgccggc gtcgagcccg acgcggccgg
     2041 gctcacggcc tggatcaacg agcgcgtggg acgtcagcaa cgtgtgaccg gcgtcgtgct
     2101 ccgggactcg ctcccgcgca acccgaatgg caagatcctc aagcgcgagc tgcgcgcgga
     2161 gtacgccgcc ggacgggcgt cgcaacggag cacgagttga acggaaccgc ctggcaggac
     2221 cgctacttcg aatcgtcgga cgggctcacg ctctacta
//
LOCUS       DASUMX010000148         2353 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_45786, whole genome shotgun sequence.
ACCESSION   DASUMX010000148 DASUMX010000000
VERSION     DASUMX010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45786"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..1352)
                     /gene="serS"
                     /locus_tag="VFZ86_07135"
     CDS             complement(96..1352)
                     /gene="serS"
                     /locus_tag="VFZ86_07135"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEX5642102.1"
                     /translation="MLDVKMVRTDPDAVRRALERRGDPTSSLDEFLAVEERRRQVLTE
                     VESRRAERKRASDEIAAVKKAGGDAGEAIAAMRALGDQIKEHEAALAETEERLKVMLL
                     ELPNIPLPDVPDGGEEDSVVLRTVGEPRQFDFAPKDHLDLGLALDLIDMERAAKVSGS
                     RFVYLKGDLVMLQFALVNYALGVIQSKGFRPVIPPVLVREEAMYGTGFFPTDRAQVYE
                     TVDGDCLVGTSEVPLAAMHMEEFLDEGTFPIRYAGYSTCFRREAGAAGRDTRGILRVH
                     QFDKVEMFSFCAPEQSAAEHQVILSAEEAILQGLGIPYQVVNIAAGDLGAPAAKKYDC
                     EAWMPGQGRYREVTSCSNCTDYQARRLNCRYRTDMGPRFVHTLNGTAVTWRFLIAIME
                     NYQRADGTIVVPKVLRPYVGKETLGG"
     gene            1687..>2353
                     /locus_tag="VFZ86_07140"
     CDS             1687..>2353
                     /locus_tag="VFZ86_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642103.1"
                     /translation="MREVVTGRVNRRFAALALVVVAAVCVLALAAPAFGSSPEPVAAS
                     SASPSPEPVAPALSAALSSASVVFGSTVTVSGTLEPAAEGQEVVVTFGGADAGTAVTD
                     ATGAYSFAFMPRRGGDVVVSLATDPAVAAAPLALAVKPKVTVSHGTLVPFLPSRFVVK
                     VAPTAYDGVIVLKVVHRRALVGTYRARVKDGRAVFKAPLRGVDGFTLTYVVPAAGGLT
                     GRTV"
BASE COUNT          301 a          838 c          829 g          385 t
ORIGIN      
        1 ttccggcgct cgatgtgagc tgctacctca tcccctggcg cgagaacggc gccgggccgg
       61 ggttcggcga cgccgagccc gtcgactgac cttcctcagc cgcccagcgt ctccttgccc
      121 acgtacgggc gcagtacctt gggcactacg atcgttccgt cggcgcgctg gtagttctcc
      181 atgatcgcga tgaggaagcg ccaggtcacg gccgtcccgt tgagggtgtg aacgaagcgc
      241 ggccccatgt cggtccggta gcggcagttg agacggcgcg cctgatagtc ggtgcagttc
      301 gagcagctcg tgacctcgcg gtagcgcccc tgaccgggca tccaggcctc gcagtcgtac
      361 ttctttgccg ccggggcgcc gaggtcgccg gcggcgatgt tgacgacctg gtaggggatg
      421 ccgaggccct gcaggatcgc ctcctcggcc gagaggatga cctggtgctc ggccgcgctc
      481 tgctcgggcg cgcagaagct gaacatctcg accttgtcga actggtgaac gcgcaggatg
      541 ccgcgcgtgt cgcggccggc ggcgccggcc tcgcgccgga agcaggtgga gtagccggcg
      601 tagcggatcg ggaacgtccc ctcgtcgagg aactcctcca tgtgcatcgc cgcgagcggc
      661 acctcgctgg tgccgaccag gcagtcgccg tccaccgtct cgtacacctg ggcgcggtcg
      721 gtggggaaga agccggtgcc gtacatggcc tcctcgcgca cgagcacggg ggggatgacg
      781 gggcggaagc ccttgctctg gatcacgccg agcgcgtagt tgaccagggc gaactgcagc
      841 atcacgaggt cgcccttgag gtagacgaag cgcgagccgc tcaccttggc cgcgcgctcc
      901 atgtcgatga ggtcgagcgc gaggcccagg tcgaggtggt ccttgggcgc gaagtcgaac
      961 tgccgcggct ccccgacggt gcggaggacg acggagtcct cctcgccgcc gtcggggacg
     1021 tccggcagcg ggatgttggg cagctccagg agcatgacct tgaggcgctc ctccgtctcc
     1081 gcgagcgccg cctcgtgctc cttgatctgg tcgccgagcg cgcgcatcgc ggcgatcgcc
     1141 tcgccggcgt cgccgccggc cttcttgacg gcggcgatct cgtcgctcgc ccgcttgcgc
     1201 tcggcgcgcc ggctctcgac ctcggtcagc acctgccggc gccgctcctc cacggcgagg
     1261 aactcgtcca gcgacgacgt ggggtcgccc cgccgttcca gggccctgcg gaccgcgtcc
     1321 gggtcggtgc gcaccatctt cacgtcgagc atcgtccact cctcgggatc acgtcggtcg
     1381 ggtcaacccg tccatggtat cgcacgccgg gcggcctcga gtcccgagcg aaggagatct
     1441 cccggagggt cggagcagct gtctggtcgc tctgttcgcg tatctacatc gtagagtcgt
     1501 gcgatgggtc ggcgcggaga aggagccgag atgtatctac gttgtagaga ccgtttcaga
     1561 gtgcccatgc aggcactcgg gcccgcccgg cgggcgcccg cacggtcgac cgtccgagag
     1621 agcccgtgct accctgctgg tatcgagcag gctcgcacag caacgcgtca cggagggagc
     1681 acgtcaatgc gcgaggtcgt caccggtcgt gtcaatcgca ggttcgcggc gctcgcgctc
     1741 gtggtggtcg cagccgtctg cgtgctcgct cttgccgccc cggccttcgg gtcgtcgccc
     1801 gagccggtcg cggcgtcgtc cgcgtcgccg tcccccgagc cggtggcgcc ggcgctcagc
     1861 gccgccctct cctcggcctc cgtcgtgttc ggcagcaccg tcaccgtgag cggcacgctc
     1921 gagccggccg ccgagggtca ggaagtcgtc gtcaccttcg gcggcgccga cgccggcacg
     1981 gcggtcaccg acgccaccgg cgcctactcg ttcgccttca tgccgcgccg cggcggcgac
     2041 gtggtcgtga gcttggccac cgacccggcg gtggccgccg cacctctggc gctcgcggtg
     2101 aagcccaagg tgaccgtctc gcacggcacg ctggtgccgt tcctcccgtc gaggttcgtc
     2161 gtcaaggtcg cgccgaccgc ctacgacggc gtgatcgtgc tcaaggtcgt gcatcgccgt
     2221 gccctcgtgg gcacctacag ggcgcgcgtc aaggacgggc gcgccgtctt caaggccccg
     2281 ctccgcggcg tcgacggctt cacgctcacc tacgtggtgc cggccgccgg cggcctcacc
     2341 gggcgcaccg tgc
//
LOCUS       DASUMX010000149         3034 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_2058594, whole genome shotgun sequence.
ACCESSION   DASUMX010000149 DASUMX010000000
VERSION     DASUMX010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3034
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2058594"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2628
                     /locus_tag="VFZ86_07145"
     CDS             <1..2628
                     /locus_tag="VFZ86_07145"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX5642104.1"
                     /translation="RRERLLNELSLKLGSSLDLREVLDSACRRICSILDAGGCEIWAR
                     REAGVVECLAAWEDGEAVEEWRGRRLSLEDWAVTRLAIEGGETVAIASTADPRLGEEE
                     RAMMREWDQRSMLVTPLRARGRVLGTLEITQSGRDRVFTAEEAATAEACARQTALAID
                     NATLYERQADHARRLQALLVAGRAVTSSLNIQDVLASLVRSAATSLGFPEALIFEYHE
                     EADAMTMRSVYEENPTVYEDLDKPYPLADYPSDRALLESDDVVVETISDPDLPADVRE
                     SMERHGEQTCLTVPLRSGASRLGMLTLVETAAERVFSEADLEFARGFGEQAAMAMHNA
                     QLFENVKGMHLGNLRALSSALTAKDIYTIGHTARVAAYAVLLAAELGWNPRRIQLLEE
                     ATYLHDIGKIAVADRVLLKSGALTDEEWALMRQHPTISAEIIEALLDEEYVAGVRHHH
                     ERYDGTGYPDGLAGKDIPVVARLLCLVDSYDAMSSRRVYRPALTHEECIGELRSCSGS
                     QFDPDMVEAFVRVLAHLDAQRAVLQAAAFEAAAAIDADDHAVLRRPQDVARAEYARIL
                     RSLRQTRRAHPEVEAMFTTAPAGELRSTIVVDSDEDDATAVPTGEVVFSDDLTLETFA
                     GRRHDANVVVVNRWGTWIAAAAPIRAGDGAVAGAVTTCRQPGNGLPAGVLKSAVSDTF
                     AEIMRTAAARQTRAEIESMTDALTGLYNHRRFHELLMETLSGERADEADIALLFCDID
                     HFKQLNDRHGHLVGDDVLRRVSRILTSSIRRGDVAARYGGDEFCLLLRGAGIDEAMEV
                     AERVRERVDQLHVAAADSGEAGVSISIGVATLSERRDGQELLAGADRAMYAAKDGGRD
                     RVVRADTLQEARPRLATQL"
BASE COUNT          432 a         1093 c         1113 g          396 t
ORIGIN      
        1 cgccgcgagc ggctgctcaa cgagctcagt ctgaagctcg gctccagcct cgacctgcgg
       61 gaggtgctcg actccgcctg ccggcgcatc tgctccatcc tcgacgccgg cggctgcgag
      121 atctgggcca ggcgcgaagc cggcgtcgtc gagtgcctcg ccgcgtggga ggacggagag
      181 gccgtcgagg agtggcgtgg ccgccggctg tctctcgagg actgggcggt gacccggctc
      241 gcgatcgagg gcggcgagac ggtcgcgatc gcctccaccg ccgacccccg tctgggcgag
      301 gaggagcgcg ccatgatgcg ggagtgggac cagcggtcga tgctcgtgac gccgctccgc
      361 gcgcggggcc gcgtcctcgg cacgctcgag atcacgcagt cgggcagaga ccgcgtcttc
      421 accgccgagg aggcggcgac cgcggaagcc tgcgcgcggc agacggcgct ggcgatcgac
      481 aacgcgaccc tctacgagcg ccaggccgat cacgcgaggc ggctgcaggc gctgctcgtg
      541 gccggccgcg ccgtgacctc ctcgctgaac atccaggacg tcctcgcttc actcgtgcgc
      601 tcggccgcga cctcgctcgg cttccccgag gcgctcatct tcgagtacca cgaagaggcc
      661 gacgccatga ccatgcgctc cgtgtacgag gagaatccga ccgtctacga ggacctggac
      721 aagccctatc ccctggccga ctatccctcg gacagggcgc tgctggagag cgatgacgtg
      781 gtggtcgaga ccatctccga cccggacctg ccggccgacg tgcgggagtc gatggagcgc
      841 cacggcgagc agacctgcct caccgtcccg ctgcggtccg gcgccagccg cctcggcatg
      901 ctgacgctcg tcgagacggc cgccgagcgc gtgttctccg aggcggacct ggagttcgcc
      961 cgggggttcg gcgagcaggc cgccatggcc atgcacaacg cgcagctgtt cgagaacgtc
     1021 aagggcatgc acctgggtaa cctgcgcgcc ctcagctcgg cgctgacggc caaggacatc
     1081 tacaccatcg gccacacggc gagagtggcg gcctacgcgg tgctgctcgc cgcggagctg
     1141 ggctggaacc cgcggcgcat ccagctgctc gaggaggcca cgtacttgca cgacatcggc
     1201 aagatcgccg tggccgaccg cgtgctgctg aagtcgggcg ccctgaccga tgaggagtgg
     1261 gcgctgatgc ggcagcaccc caccatcagc gccgagatca tcgaggccct gctggacgag
     1321 gagtacgtgg ccggcgtgcg ccaccaccac gagcgctacg acgggaccgg ctatccggac
     1381 ggcctcgccg gcaaggacat ccccgtcgtc gcccggctgc tgtgcctcgt cgactcctac
     1441 gacgccatgt cgtcgcgccg ggtgtaccgg ccggcgctga cgcacgagga gtgcatcggg
     1501 gagctgcgca gctgcagcgg ctcgcagttc gacccggaca tggtggaggc gttcgtccgc
     1561 gtcctcgctc acctggacgc gcagcgcgca gtgctgcagg ccgccgcctt cgaggcggcg
     1621 gccgccatcg atgcggacga ccacgccgtc ctgcgccggc cgcaggacgt ggcccgcgcc
     1681 gagtacgcgc gcatcctgcg ctccctgcgc cagacgcggc gcgcgcaccc cgaggtggag
     1741 gccatgttca cgacggcgcc ggcgggcgag ctgcgcagca cgatcgtggt ggactcggac
     1801 gaggacgacg ccacggcggt gccgaccggc gaggtggtct tctcggacga cctcacgctc
     1861 gagacgttcg ccggccgccg ccacgacgcg aacgtcgtcg tcgtcaaccg ctggggcacg
     1921 tggatcgcgg cggcggcgcc gatccgcgcc ggcgacggcg ccgtcgccgg cgcggtcacg
     1981 acctgccggc agcccggcaa cggcctgccg gccggcgtgc tcaagagcgc cgtgagcgac
     2041 accttcgcgg agatcatgcg cacggcggcg gcacggcaga cgcgcgcgga gatcgagtcc
     2101 atgaccgacg cgctgaccgg cctctacaac caccgccggt tccacgagct gctcatggag
     2161 acgctgtcgg gcgagcgcgc ggacgaggcc gacatcgcgc tgctgttctg cgacatcgac
     2221 cacttcaagc agctcaacga ccgtcacggg cacctcgtgg gcgacgacgt cctgcgccgc
     2281 gtgagccgga tcctgacctc gtcgatccgg cgtggcgacg tcgccgcccg gtacggcggc
     2341 gacgagttct gcctgctgct gcgcggcgcc gggatcgacg aggcgatgga ggtcgccgag
     2401 cgcgtgcgcg agcgcgtcga ccagctgcac gtggcggctg ccgactccgg cgaggccggc
     2461 gtgagcatca gcatcggcgt cgcgacgctg tccgaacgcc gcgacggcca ggagctgctc
     2521 gccggcgcgg accgcgccat gtacgcggcc aaggacggcg gccgcgaccg cgttgtgcgg
     2581 gccgacacgc tgcaggaggc gcggccgcgc ctggcgacgc agctgtgagg cgccgccggg
     2641 gacggcactg tcccgcagct gagtggcgcc acccatgacg ccaccggtga tgccactcgt
     2701 cgcaggacac tgacagctcc ggccgtcccg ggggcgaggt cgcgcgtgat ccgcgcgagc
     2761 accctgagtg ctgcgcggac tcgcgcgatc gggcgtactc atggaagcac tcttgtacct
     2821 tggcgcgtac gtccgggcgt gcgacagcgt gcggcgctac cgcgcgcggt tcgtgcgctg
     2881 cctcgagccg gtcgaagcgg tggcggcgcg gcggcgtccg cggcgcgtcg gcccgccgcc
     2941 ggcgccgccg tgcgccggcg gcgctggtgt acgatggcac cgtccccgag gagggaggga
     3001 cgagaatggt cacgctgctc gactcgcgcg ccat
//
LOCUS       DASUMX010000150         3697 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1052392, whole genome shotgun sequence.
ACCESSION   DASUMX010000150 DASUMX010000000
VERSION     DASUMX010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3697
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1052392"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1360
                     /locus_tag="VFZ86_07150"
     CDS             <1..1360
                     /locus_tag="VFZ86_07150"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5642105.1"
                     /translation="EEHLQRDRRAVEGIAAELAEAASGKEAANATAANALLQSEQLRP
                     AVEEAEAALHRSEAAHSSALALVTRRRVELEERLAAAARVAEQREAARRRAAADRERL
                     RELERRLAGIPDVRAACATVEARTGALRAHSTRLIAQLDVGDEEGAGLERGELRKLAD
                     DEAQLRRGLENAGERRTEVQVALARLEDRRGELAASLEEVSERIDQAGFAPPADAAEE
                     AGLRERVERLSRRRERIGPVNPLAEAECAELSERAAFLREQRRDLEKSMDDLDALIKE
                     LTAQVDAEFAATFAAVREQFSHMVAVLFPGGRGQLKLVEPDEDHPQGGVAVEVKPAKK
                     LGKRLQLLSGGERALVAIAFLMALVLARPCPFYVLDEIEAALDDVNIGRLVQLLRDYR
                     ERTQFVIITHQKRTMEAADVLYGVTMGQDGASQVVSARMAEEEIEREERARAKQPGAP
                     AD"
     gene            1407..2441
                     /gene="ftsY"
                     /locus_tag="VFZ86_07155"
     CDS             1407..2441
                     /gene="ftsY"
                     /locus_tag="VFZ86_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019225651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEX5642106.1"
                     /translation="MEWHEVFKGVVEAEVPAEVLEEEAQERRGLFGRLRRNLGKAHGA
                     VTDALKSAVYVGVDRRLYEQTEEMLIAADVGVDGTIKIVDELEQRCTAKKIETREPFQ
                     DELADVVAELLRPDDPEQQRIDVSHQPTVILMVGVNGTGKTTTIGKIAWRLKEMGKTP
                     LMVAGDTFRAAAVEQLSQWADRVGCEIVKQAPDADPAAVVFDGVAAARSRGADVVLVD
                     TAGRLHTQVNLMRELEKVRRVIEKQLPGAPHETLLVLDATTGQNGLRQAQEFKQAVDI
                     SGVVLTKLDGTAKGGIVVAIHESLGIPIKLIGVGEQLQDLRPFEPDIFARAIFGSAEG
                     YASAGDIAAG"
     gene            2727..>3697
                     /locus_tag="VFZ86_07160"
     CDS             2727..>3697
                     /locus_tag="VFZ86_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642107.1"
                     /translation="MQKEVYATIMAMSASPEHSTSDRIEALTKGHGMLNIAALSSANA
                     MAFEILRGRDTKIVDANLSELALDYVVEVGVKAAAEAGADKANAALIAATLLLIAGTE
                     SRAGVPAGNRKLGAMARIKAGADRSGVAAVPTSKLTNKLSGFAAVQALYEAMQRGDLV
                     RVDGADVPAFVAGGAPMGHSVLGEDMVYIDLCVNGTKIAVDAMIQAYHGVGISASPIQ
                     CAMLAAAAVLEIVNPDGMISPEEGDFFVHGTGYLAGKGAAQAAGLPEKLHLRGTGKEF
                     DTASLVGGLGMMLKDVGAPSVIGMMTLNEMLAAFAESAMIGAGFGGGP"
BASE COUNT          593 a         1257 c         1371 g          476 t
ORIGIN      
        1 cgaggagcac ctgcagcgtg accggcgcgc cgtggagggc atcgccgccg agctggccga
       61 ggcagcctcc ggcaaggagg ccgcgaacgc gacggcggcg aacgcgctgc tgcagtccga
      121 gcagctgcgc ccggccgtgg aagaggccga ggcggctctg caccggtccg aggccgcgca
      181 ttcgtccgca ctcgccctcg tgacccggcg tcgcgtggag ctcgaggagc gcctcgccgc
      241 cgccgcgcgc gtggccgagc agcgcgaggc cgcccgccgc cgcgccgccg ccgaccgcga
      301 gcgcctaaga gagctcgagc ggcgcctcgc cggcatcccg gacgtgcgcg ccgcctgcgc
      361 gacggtggag gcgcggaccg gcgcgctccg ggcgcactcc acgcgcctga tcgcccagct
      421 cgacgtgggt gatgaggagg gcgccggcct ggagcgtggc gaactccgca agctggccga
      481 cgacgaggcg cagctgcgtc gcggcctgga gaacgccggc gagcgtcgca cggaggtcca
      541 ggtcgcgctc gcccggctcg aggaccgccg cggcgagctc gccgccagtc tcgaagaggt
      601 gagtgagcgg atcgaccagg cggggttcgc gccgccggcg gatgcggccg aggaggcggg
      661 gctgcgggag cgcgtcgagc gcctgtctcg ccgccgcgag cgcatcgggc cggtcaatcc
      721 gctggccgag gccgagtgcg cggagctctc ggagcgcgcc gccttcctgc gcgagcagcg
      781 gcgcgacctc gagaagtcca tggacgacct cgacgcgctc atcaaggagc tcaccgcgca
      841 ggtcgacgcc gagttcgcgg cgaccttcgc cgccgtgcgg gagcagttca gccacatggt
      901 ggcggtgctc ttcccgggtg gtcgcggtca gctgaagctg gtcgagcccg acgaggacca
      961 cccgcagggc ggggtggccg tcgaggtcaa gcccgccaag aagctgggca agcgcctgca
     1021 gttgctctcc ggcggcgagc gcgccctggt ggcgatcgcc ttcctcatgg ccctcgtgct
     1081 ggcgcgtccg tgcccgttct acgtgctcga cgagatcgag gcggcgctgg acgacgtcaa
     1141 catcggccgc ctggtgcagt tgctccgcga ctaccgcgag cgcacgcagt tcgtgatcat
     1201 cacccaccag aagcgcacca tggaggccgc ggacgtgctg tacggcgtga ccatggggca
     1261 ggacggggcg tcccaggtgg tgagcgcgcg catggcggaa gaagagatcg agcgcgagga
     1321 gcgcgccagg gcgaagcagc cgggcgcgcc ggcggactga aggcgaccgg ccgcccttgg
     1381 ggggccgttg acgggagact gacagggtgg agtggcacga ggtgttcaag ggcgtcgtcg
     1441 aggcggaggt gccggcggag gtcctggagg aagaagcaca ggagcgcaga gggctcttcg
     1501 gccgcctgcg ccgcaacctg ggcaaggcgc acggcgccgt cacggacgcg ctcaagagcg
     1561 ccgtctacgt cggcgtcgac cgccggctgt acgagcagac cgaggagatg ctgatcgccg
     1621 ccgacgtggg cgtcgacggc accatcaaga tcgtcgacga gctcgagcag cgctgcacgg
     1681 cgaagaagat cgagaccagg gagccgttcc aggacgagct ggcggacgtg gtcgccgagc
     1741 tgctgcggcc ggacgacccg gagcagcagc gcatcgacgt ctctcaccag ccgacggtga
     1801 tcctcatggt cggcgtcaac ggcaccggca agaccacgac catcggcaag atcgcctggc
     1861 gtctcaagga gatggggaag acgccgctca tggtggccgg cgacacgttc cgcgccgcgg
     1921 cggtggagca gctgagccag tgggccgacc gcgtgggctg cgagatcgtc aagcaggcac
     1981 ccgatgccga ccccgcggcc gtggtgttcg acggcgtggc cgccgcgcgc tcgcgcggcg
     2041 ccgacgtcgt gctcgtcgac acggccggcc gcctgcacac gcaggtgaac ctgatgcgcg
     2101 agctcgagaa ggtgcgccgg gtcatcgaga agcagctgcc cggcgccccg cacgagacgc
     2161 tgctggtgct cgacgccacc accgggcaga acggcctgcg ccaggcgcag gagttcaagc
     2221 aggccgtgga catcagcggt gtggtgctga ccaagctcga cggcaccgcc aagggcggca
     2281 tcgtggtggc catccacgag tcgctcggca tcccgatcaa gctcatcggc gtcggcgagc
     2341 aactccagga cctgcggccg ttcgagccgg acatcttcgc gcgggcgatc ttcggctcgg
     2401 ccgagggcta cgcgtccgcc ggcgacatcg ccgccggctg acgggctcga tctcgcgcgg
     2461 gcgctcgcga tgccgcgatg atccaaggga gtctgcccct ggaccgagca ctccgtcgtg
     2521 cttctctggt cggcctgtgg gacgacgcat ataatgagcc cctgcccaca acgggggtgc
     2581 gtcggcaagc agcgccgttg gtctgccgat cgtgcagacc cccgcattct gagggcacgc
     2641 gaccaagcgg aaccaggggg tgacactccc gccgttccgc tcaagcggct tggttcgaag
     2701 cgagccggaa cagtggaggt accgaggtgc agaaggaagt ctatgcgacg atcatggcga
     2761 tgtcggccag tccggagcat tctacgagtg acaggatcga ggcgctgacc aagggccacg
     2821 ggatgctgaa catcgcggcc ctgagctcag ccaacgcgat ggccttcgag atcctccgcg
     2881 gccgcgacac caagatcgtc gacgccaacc tgtctgaact ggcgctcgac tacgtcgtcg
     2941 aggtcggggt caaggcggcg gccgaggcgg gcgccgacaa ggcgaacgcc gccctcattg
     3001 cagccacgct cctgctcatc gcgggcacgg agtcgcgcgc cggcgtaccg gcgggcaacc
     3061 gcaagctcgg agcgatggcg cgcatcaagg ctggtgccga tcggtccggc gtcgccgcgg
     3121 tccccacctc caaactcacg aacaagctgt ccggcttcgc cgccgtacag gcgctctacg
     3181 aggccatgca gcgcggagac ctggtccgcg ttgacggcgc cgacgtcccg gcgttcgtcg
     3241 ccggcggcgc cccgatgggc cacagcgtgc tgggcgagga catggtgtac atcgatctct
     3301 gcgtcaacgg caccaagatc gccgtcgacg cgatgatcca ggcgtaccac ggggtcggga
     3361 tcagcgcgag ccccatacag tgcgccatgc tcgccgccgc cgcggttctc gagatcgtca
     3421 atccggacgg catgatcagc cccgaagagg gcgacttctt cgtgcacggc acggggtacc
     3481 tggccggcaa gggcgccgcg caggccgcgg gcctgcccga gaagctgcat cttcgcggca
     3541 ccggcaagga gttcgatacc gccagcctcg tgggcggcct aggcatgatg ctcaaggacg
     3601 tcggggcgcc gtcggtgatc ggcatgatga cgctcaacga gatgctggca gccttcgccg
     3661 agagcgccat gatcggcgcc ggtttcggcg gcggccc
//
LOCUS       DASUMX010000151        10992 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_2060158, whole genome shotgun sequence.
ACCESSION   DASUMX010000151 DASUMX010000000
VERSION     DASUMX010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 10992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10992
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2060158"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            10..132
                     /locus_tag="VFZ86_07165"
     CDS             10..132
                     /locus_tag="VFZ86_07165"
                     /inference="COORDINATES: protein motif:HMM:NF013379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEX5642108.1"
                     /translation="MADDPAAEEDLKRFAKRAGHEVVSLENDGVELRILLRRLK"
     gene            150..512
                     /locus_tag="VFZ86_07170"
     CDS             150..512
                     /locus_tag="VFZ86_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HEX5642109.1"
                     /translation="MAEEKNILYVQTSDLAERQYSPLVLAQSAKAMGVDAAVYYLGMG
                     LTVLKPGAAEEMQLGSFPSVAEMLKKTLDMGITVYVCEASKQMLGWDSVELIDGVKIV
                     GAATLNDLALDADATMWF"
     gene            522..1760
                     /locus_tag="VFZ86_07175"
     CDS             522..1760
                     /locus_tag="VFZ86_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048064599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HEX5642110.1"
                     /translation="MTDARGTALQIGDPGEALRRDGIVYADYQSAKPVDPRVVAAMLP
                     YLTEKFGNAASLHGVGDVATAALEEARGRVAAFFGADPREIVFTGGATEASNLALAGY
                     ALRNRRKGDHVIISEAEHISVLNIAKYLEKQGFRVTRAPIDLHGRVSPKKLRARITDE
                     TILVSLQWASSEIGTVQPIAEIAEMLDGTGIALHSDAVAAEGLIPIDTGAVPISLLSL
                     SSNDVYGPRGLGALYVRKGVQVAPVLLGGGQERGLRSGSEDLASIAGFAAAADIMAAE
                     MPAEVERIRAIRDALVERVLAEIPDAHLNGHPEQRLPNNAHFRFHGVEGEAMVLSLRD
                     AGIAASTGSACSSKTLEPSHTLISCGLLHEEAHGSLEFTFGRWSRMEDVDTIMAALPG
                     VISRLRALSPLYKTHAPATP"
     gene            1792..2160
                     /locus_tag="VFZ86_07180"
                     /pseudo
     CDS             1792..2160
                     /locus_tag="VFZ86_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695927.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
     gene            complement(2702..3280)
                     /locus_tag="VFZ86_07185"
     CDS             complement(2702..3280)
                     /locus_tag="VFZ86_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005035118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HEX5642111.1"
                     /translation="MAELKGTRTEENLLKAFAGESQARNRYTFYAKEAQKQGYEQISG
                     IFLETADHERQHAKRFYKHLQGGTVEITAAYPAGPLEGTTAEHLAGAAAGEHEEWAEL
                     YPAFAEVAEAEGFKDVATTFRMVAKAEAMHEERYRKLLANVETGAVFEKPEKVRWMCR
                     KCGYIHEGPKAPKMCPTCLHPQPYFQVFAENY"
     gene            complement(3514..4479)
                     /locus_tag="VFZ86_07190"
     CDS             complement(3514..4479)
                     /locus_tag="VFZ86_07190"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX5642112.1"
                     /translation="MGEPTGERGAGAPVLSAEGPARSADLPPQGPARRDAAFAAALVV
                     VLTLSIQTGSALAVRVIESVGVFEALWLRTAFAALILIIVRPGALRRLPPKGHRLPLA
                     ALAVTLFLMNLSFYGAISLVPVGIVVAIEFVGPLGVAVLGTRNRLDWLWIALAGAGVV
                     VLAGPSGSATGLGLLLALSAGICWGVYLLLAKRAVTGMDPLSVTALMMVGATVLATPL
                     LAIDGVRLAGHWNAIALGIVIAVVSSAFPYWLEMVAIRRVRAATYGVLLSIEPAVAAL
                     AALAVLGQRISPLEAAAMGAVMAAAAGASWTSGGAAEAGVELPAP"
     gene            4637..4963
                     /locus_tag="VFZ86_07195"
                     /pseudo
     CDS             4637..4963
                     /locus_tag="VFZ86_07195"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
     gene            5063..5908
                     /locus_tag="VFZ86_07200"
                     /pseudo
     CDS             5063..5908
                     /locus_tag="VFZ86_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923785.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
     gene            6148..6636
                     /locus_tag="VFZ86_07205"
     CDS             6148..6636
                     /locus_tag="VFZ86_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX5642113.1"
                     /translation="MFRGMRRKRQVLTEADTIAMLRSCTSGVLAVHGDDGYPYAVPLS
                     FAHEDGKLFFHSAKAGHKIDAIGRDEKASFCVIAADDVVQSTFTTHFRSAIVFGRVRL
                     VTEDGEKRHALECLAEKYSPEHLEAADAEIERDWKRVCVIELAIEHMTGKAAIEIVKE
                     TA"
     gene            complement(6676..7425)
                     /locus_tag="VFZ86_07210"
     CDS             complement(6676..7425)
                     /locus_tag="VFZ86_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HEX5642114.1"
                     /translation="MERRVATIDGVAVEHAVAGNGTPAIVLLNGAGGPIEGWYRVLAP
                     LAAVSTVVAYNRLGIGRSSKPSEPQTGDVVVRLLHALLQKLGLRPPHVLVGHSLGGLF
                     VDLFARTYPDEVAAVVFLEAAAPEDSTLVDEHGTGFQRSAQRLVDAVFARDQFGEAAC
                     VPQTVDMIARAGAFPPVPVVVVSGGKPARGVPAAVRDARARNQAGLVALSPLGEHLVA
                     RRSGHFPQMSEPGLVSDAIRSVVASVRERNG"
     gene            complement(7460..8437)
                     /locus_tag="VFZ86_07215"
     CDS             complement(7460..8437)
                     /locus_tag="VFZ86_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642115.1"
                     /translation="MEMPIDWLLEGDPWIEYRTRRDLLGRPEDDLEVLDARESMLASP
                     QVRHVLAELAEWPGSVVSSHKNAGLLLHKLTFVADLGLRVGDPGVDEVVARVLEHRSA
                     DGPFQVPMNIPAHFGGTGLDQPAWALCDTPLIVSSLTRFGLGRQRVVQAAIEYLEALI
                     RDNGWPCAVSKELGAFRGPGRKDDPCPFANLAMLKALSASPALRETAPCHVGANTLLT
                     LWADSTTRHPYQFYMGTDFRKLKAPLVWYDLLHVLDVLSRFSWLQDDPRLLDMLALLK
                     GKADRQGRFTPESVWMAWRGWEFAQKKEPSRWLTLLAWRIIQRVETGSA"
     gene            8701..8976
                     /locus_tag="VFZ86_07220"
     CDS             8701..8976
                     /locus_tag="VFZ86_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642116.1"
                     /translation="MTMDRGCPGQDRSNWTPDDIFEAPCSNCGALIEFFKDDAKRYCA
                     ACGTCNPNPRQDIGCAAWCAAADKCSTGRQWLEEDSLADTPAFGDPA"
     gene            complement(9089..10009)
                     /locus_tag="VFZ86_07225"
     CDS             complement(9089..10009)
                     /locus_tag="VFZ86_07225"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEX5642117.1"
                     /translation="MLIIGENIHIIAPPVKAAIEARDAGTIQAMAVAQVEAGAGIVDL
                     NIGPQKKLGHEVMPWIVQAVQEVVDTPLSLDTTNLAAMEAGLKVCKQPAMLNSASADP
                     DRLDSVMQLAARYGAKVIALTMGVDGIPTTADGRAGIAMEALLPAAEAAGIPVADVFL
                     DPLVLAVNCNQDVAMETVEAVRMFKMMADPPPTTVIGLSNISNGALPEVRPLINRVMM
                     VMAMGAGLDSAIADPLDGVQNEWIRIAEQRDESTPLGKLVVALYDAVAAMDKLDPALV
                     DLDDQEQLELWKTYRMLTNETLYADSYLKL"
     gene            complement(9993..10793)
                     /locus_tag="VFZ86_07230"
     CDS             complement(9993..10793)
                     /locus_tag="VFZ86_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5642118.1"
                     /translation="MTTTIAVAGKGGTGKTTLAGLLIRRLAEVQAGPILAIDADPASN
                     LNTVLDLPLEKTVGDVREETSEKARANLLEAGIAKNDLLDYEINSSLVEGVNVDLLAM
                     GRPEGPGCYCAANNMLRLIIDKIADSYPWVVIDNEAGLEHLSRRTTRDVDVLFIVSDA
                     TIRGVTTAGRVATLLGELRTKVGRHYLIVNRAASGLSPELEAAIAEHGLELLAVLPDD
                     PAVAEFDAAGRPLVGLPADSLLSTALEPVVAAALATPQEKEAAGAHHR"
     gene            complement(10797..>10992)
                     /locus_tag="VFZ86_07235"
     CDS             complement(10797..>10992)
                     /locus_tag="VFZ86_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014794085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HEX5642119.1"
                     /translation="LLCVNVRREVADVASKMTYLELSADNTFMDEYTSALFLPHTDLT
                     GFPSVRAALAERSHGSGNGG"
BASE COUNT         1784 a         3879 c         3778 g         1551 t
ORIGIN      
        1 ctggaggtca tcgccgacga tcccgccgcc gaggaggacc tcaagcgctt cgccaagcgc
       61 gccggtcacg aggtcgtctc gctcgagaac gacggcgtcg agctccgcat cctcctccgc
      121 aggctcaagt agaagacgaa aggatcacca tggccgaaga gaagaacatc ctctacgtac
      181 agaccagcga cctcgcggaa cgccagtact cccccctcgt gctcgcgcag agcgccaagg
      241 cgatgggcgt cgacgcggcc gtctactacc tgggcatggg cctgacggtg ctcaagcccg
      301 gcgccgccga agagatgcag ctcgggagct tcccgagcgt ggcggagatg ctcaagaaga
      361 cgctggacat gggcatcacc gtgtacgtct gcgaagcctc caagcagatg ctcggctggg
      421 actccgtgga gctcatcgac ggcgtcaaga tcgtcggcgc ggcgacgctc aacgacctcg
      481 ccctggacgc cgacgcgacg atgtggttct gaggcgccgc gatgactgac gcccggggca
      541 cagccctcca gatcggcgac cccggcgagg cgctgcgccg cgacgggatc gtctacgccg
      601 actaccagag cgccaagccc gtcgacccgc gcgtggtcgc ggcgatgctg ccgtacctca
      661 ccgagaagtt cggcaacgcc gcgtcgctgc acggcgtcgg cgacgtggcg acggccgcgc
      721 tcgaggaggc gcgcgggcgc gtcgccgcgt tcttcggcgc ggacccgcgc gagatcgtct
      781 tcaccggcgg cgccaccgag gccagcaacc tcgcgctcgc cggctacgcc ctgcgcaacc
      841 ggcgcaaggg cgatcacgtg atcatcagcg aggccgagca catcagcgtg ctcaacatcg
      901 ccaagtacct cgagaagcag ggcttccgcg tgacgcgggc cccgatcgac ctgcacgggc
      961 gcgtgagtcc caagaagctg cgcgcccgca tcaccgacga gaccatcctc gtctcgctcc
     1021 agtgggcgag cagcgagatc ggcaccgtcc agcccatcgc ggagatcgcc gagatgctgg
     1081 acggcacggg catcgccctg cacagcgacg ccgtggccgc cgaggggctc atccccatcg
     1141 acacgggcgc cgtgccgatc agcctgctga gcctctcgag caacgacgtc tacggtcccc
     1201 gcgggctcgg cgccctctac gtccgcaagg gcgtgcaggt cgcgccggtg ctgctcggcg
     1261 gcggccagga gcgcggcctg cgctccggca gcgaggacct cgcgagcatc gcgggcttcg
     1321 ccgcggcggc cgacatcatg gccgcggaga tgcccgccga ggtcgagcgc atccgcgcca
     1381 tccgcgacgc gctggtcgag cgcgtgctgg ccgagatccc ggacgcgcac ctgaacggcc
     1441 accccgagca gcggctgccg aacaacgcgc acttccgctt ccacggcgtg gagggagagg
     1501 cgatggtcct gtcgctgcgc gacgccggca tcgccgcgtc caccggctcc gcctgcagct
     1561 ccaagacgct ggagccgtcg cacacgctca tctcctgcgg cctgctgcac gaagaggcgc
     1621 acggctcgct cgagttcacc ttcgggcgct ggagccgcat ggaggacgtg gacaccatca
     1681 tggccgcgct ccccggcgtg atctcccgcc tgcgcgcgct ctcgcccctg tacaagacgc
     1741 atgctcccgc gacgccgtga ccccggcgca gacaccgaaa ggcgacgacc gatgaaatcg
     1801 acgcagtaca ccgagaagac gctcgagcac ttccgcgacc cgcgcaacgt gggcacgctg
     1861 gagggcccga acgtcgccgt cggccgcgtg ggcaacccgg tctgcggcga catgatggac
     1921 atctacatcg aggtcgagga cgaccgcatc aaggacatca agttccagac cttcggctgc
     1981 ggctccgccg tagccacctc gagcatgatc accgagctcg tcatgggcat gcagctcgac
     2041 gacgcgctca aggtgagccg cggcgacgtc gccgacgcgc tcgacggcct gccgcccatc
     2101 aagatgcact gcagcaacct ggcggcggac gcgctgcacg aggcgatcaa gaactggcgc
     2161 gagggcgtgg gcccggagga cgccaagaag acgccgggcg gcaccgcctg tcacccgatc
     2221 ccggcgcagg tcgccgaggt cgagatccag aagctcgacg agtacgacgg caagggcctc
     2281 taccgcaagc tcgacgacct caccgacatc gcggaccagc gcgtcgtggt gctcgacacc
     2341 ggcgacgacg cggcccggct ggcgatggac ctcaccgagc acaccggccg cgtcgtcctg
     2401 atgacgaccg gcaaggagcc gagcgtcagc cccgcggtgc gctcggcgct gcgcctcagc
     2461 gacgtcaagg tgctggccga ggcgcagccg ctcgagctgc tcggtgaagg cgagctcgag
     2521 cgcatcaggg tccacgacct caacgaggac gaggaatacg agctcacggc cgacgcgttc
     2581 gtggtgctcg agtagcccgg aagaggagct gccgcgcgcc gcgaggcgcc gcggcgcccg
     2641 tacgcgacga cggggcgggt ggcccttgcg gcctcccgcc ccgtcgcgcg tacgcggtca
     2701 ctcagtagtt ctcggcgaac acctggaagt acggctgcgg atgcaggcag gtcgggcaca
     2761 tcttgggcgc cttcgggccc tcgtggatgt agccgcactt gcggcacatc cagcgcacct
     2821 tctccggctt ctcgaagacc gcgcccgtct ccacgttggc cagcagcttg cggtagcgct
     2881 cctcgtgcat cgcctcggcc ttggccacca tgcggaaggt ggtggccacg tccttgaagc
     2941 cctcggcctc ggccacctcg gcaaaggcgg ggtacagctc cgcccactcc tcgtgctcac
     3001 cggcggccgc gccggccagg tgctcggccg tggtgccctc cagcggaccc gccgggtacg
     3061 ccgcggtgat ctcgaccgtg ccgccctgga ggtgcttgta gaagcgcttg gcgtgctgac
     3121 gctcgtgatc cgcggtctcg aggaagatgc cgctgatctg ctcgtagcct tgcttctgcg
     3181 cctctttggc gtagaacgtg tagcgattgc gcgcctggct ctcgccggcg aacgccttga
     3241 ggaggttctc ctccgtcctg gtgcccttga gctcggccat cctgacccct ttcgtcgtgt
     3301 cgctgcgtgg gattctatcg tccgggcacg ccgagccgcg agagagaagg cgaagcggag
     3361 tggcgtcact ggtggcgtca cccatgacgc catccgtgac gccacccgcc ggcggacgac
     3421 cccgagccgg ccgacctgcg cgcgacgacc cggcgggcga ccccgggccg ggctgagggc
     3481 gacgacccgg cggcgaccag cgcgaggcga gcgtcagggc gccgggagct ccacgcccgc
     3541 ctcggcggcg ccgccgctcg tccagctggc gccggcggcg gcggccatga ccgcgcccat
     3601 cgccgccgcc tcgagcggcg agatgcgctg gccgagcacg gcgagcgcgg ccagcgcggc
     3661 gacggcaggc tcgatgctga gcagcacgcc gtaggtggcg gcgcggacgc ggcggatcgc
     3721 caccatctcc agccagtacg ggaaggcgga cgagaccacg gcgatgacga tgccgagcgc
     3781 gatcgcgttc cagtggcccg ccagcctcac gccgtcgatc gccagcagcg gcgtcgccag
     3841 gacggtggcc ccgaccatca tgagcgccgt gacggagagc gggtccatcc cggtcacggc
     3901 gcgcttggcg aggagcaggt acacgcccca gcagatgccg gccgacagcg cgaggagcag
     3961 cccgagaccc gtggcggagc cggaagggcc ggccaggacg acgacgcccg cgccggccag
     4021 cgcgatccac agccagtcga gccggttgcg cgtgccgagc acggcgacgc cgagcggccc
     4081 gacgaactcg atggcgacga cgatccctac cggcacgagg ctgatggcgc cgtagaagct
     4141 caggttcatc aggaacaggg tcacggcgag cgccgcgagc ggcaggcgat ggcccttggg
     4201 cggcagccgg cgcagcgcgc ccggccgcac gatgatgagg atcagcgccg cgaacgccgt
     4261 gcgcagccac aacgcctcga agacgcccac ggactcgatg acgcgcacgg cgagggccga
     4321 ccccgtctgg atggagaggg tgaggacgac gacgagcgcg gcggcgaagg ccgcgtcgcg
     4381 ccgcgccggc ccctgcggag gcaggtctgc ggaccgcgcc ggcccctccg ccgacagcac
     4441 cggcgcgccg gcccctcgtt cacccgtggg ttcgcccatc ccctgagtct agctcagccc
     4501 gggccggctc acgggcggtc gcgccacgca tccggggctg ccggcaacgc ttgtgggcgg
     4561 catgccggca tggtactttt tcacccttcc ccagttgcgg atgaaaattg tccgcttcac
     4621 cgacagcgag ggcgacatga gcgcgaggcc gaagaccccc acccatgccg acgagcagca
     4681 cgccgtcctg tacggccatc ccatcccgga gaacagcttc gccgtacgag acctggtcca
     4741 caagctcccc ggtgtcggcc tgttcgacct gcgccggacc gaggacggcg cccacgcctg
     4801 caccttctgc ggcgcctgcc agaaggcctg tcctgtggac tgcatcgacg tgcgccgcga
     4861 cccctcgcgc tcgcacggca agggcaagga gcgccacttc gccggcacca tcacgctcga
     4921 cgagcggcgg tgcatgacct gcaaccactg catggagacc tgcaggttcg acgcgctggt
     4981 ggccaccgat ctgtaccggg agcaccgtcg tcgcgtgcag gagctggcgc acgagacgcc
     5041 gcagctctcc agcggtcacg gcatgtgcag cggctgcacg gcgccggtcg tggtcaatca
     5101 ggtgctgagc caggtcaaga gccactacgt cgtctccggg gccaccggct gcctcgaggt
     5161 gtccaccacg cgctacccct acaccgcctg gaagggcagc tacatccaca ccaacttcga
     5221 gaacgccgcg gcgacgctct cgggcgtcga gaccgccttc cgttccctgt acaagcgcgg
     5281 caagctcgag gagcgggtca agttcatcgc cttcggcggc gacggcggca cctacgacat
     5341 cggcctgcag gcgctctccg gcgccatgga gcgcggccac gacatgctct acgtgtgcta
     5401 cgacaacggc ggctacatga acaccggctt ccagcgctcg agcgcgacgc cacacggcgc
     5461 ctggaccacc accagtccgg tcggcgacga gctccccggc aagctcggcc acagcaagaa
     5521 cctcaccgag atcatggtgg cgcaccgcct gccttacgtc gcccagtcct cgccgcacga
     5581 cccgcaggac ctcacgcaca aggcgtccaa ggcgctggag atcgaggggc ccaccttcct
     5641 caacgtgctc tccgcctgcc cgcgcggctg gcgcaccgac aacgacgaag gcatcgacct
     5701 cagccgcctc gccacagaga cctgctactg gccgctgttc gaggtcaacg aaggcgtgta
     5761 tcgcctcacc taccggccgc ggcacaagca gccgctgggc aagtggctgg agcgccaggg
     5821 gaggttcgcc cacctggcgc tcccgggcaa cgagcacatg gccgaggcgc tgcaggattg
     5881 ggtcgacttc gagtgggacc tgctgctgcg caagtgcgac gagatccccg aggaccagtg
     5941 ggacagggcg agcagccgtc acaaggccga gccgttcgtg atgcacccga gccgcgcgca
     6001 cctggagtcg ctgggcgagc cgctcaccgc gagcatgacc tccaccgcgc tcgactggga
     6061 cgacttcgac tggggccgcc actcgggcat gcaggacggc gcgttcgagg attagcgtgt
     6121 gcgcagccgc gcaagagagg gggagacgtg ttcaggggca tgaggcgcaa gaggcaagtc
     6181 ctcacggagg cggacacgat cgccatgctg cggtcctgca cctcgggggt cctggcggtg
     6241 cacggcgatg acggctatcc ctatgcggtc cccctgagct tcgcccacga agacggcaag
     6301 ctcttcttcc actccgcgaa ggccggccac aagatcgacg ccatcgggcg tgacgaaaag
     6361 gcctccttct gcgtcatcgc tgcagatgac gtcgtgcaga gcacgttcac cacgcacttc
     6421 cgcagcgcga tcgtcttcgg cagagtgagg ctcgtcacgg aggacggcga gaagagacac
     6481 gccctcgagt gcctcgccga gaagtactca cccgagcacc tcgaagcggc ggacgccgag
     6541 atcgagcgcg actggaagag agtgtgcgtg atagagctcg ccatcgagca catgaccggc
     6601 aaggccgcca tcgagatcgt caaggagacc gcctagacac cgccggcgga cccgcagcgc
     6661 atcagcctga tccggtcagc cgttgcgctc cctgaccgag gccaccaccg atcgtatcgc
     6721 gtcgctgacg agacccggct cgctcatctg tgggaagtgg ccgctcctgc gggcgaccag
     6781 atgctcaccc agcggcgaaa gggcgaccag tcccgcttga ttccgggccc gcgcgtcacg
     6841 gaccgcggca ggaacgccgc gcgcgggctt gcctccgctg acgaccacga ccggcacggg
     6901 cgggaaggcg cccgctcgcg cgatcatgtc cacggtctgc gggacgcacg cggcctcgcc
     6961 gaactggtcc cgcgcgaaca ccgcatccac cagccgttgc gcagaccgct ggaagccggt
     7021 gccgtgttca tcgaccagcg tggaatcctc gggcgccgcg gcctccagga agacgacggc
     7081 agccacctca tcggggtacg tgcgcgcgaa caggtcgacg aacagaccgc caagagagtg
     7141 gccgacgaga acgtggggag ggcgcagacc cagcttctga agcagcgcgt ggagcaggcg
     7201 caccacgacg tctccggtct gcggctctga gggcttgctg cttcgaccga tgccgagccg
     7261 gttgtacgcg acgacggtgc tcacggccgc gagcggcgca aggactcggt accagccctc
     7321 gatcgggcct cccgcgccgt tgagcagaac gatggcgggc gtaccgttgc cggctacggc
     7381 atgctcgact gccacgccgt cgatggtggc gacacgcctc tccacgaacc ccttactcca
     7441 agcctttgtc gaacggagac tacgccgatc ctgtctccac cctctggatg atccgccacg
     7501 ccagcaacgt gagccagcgc gagggttcct tcttctgcgc gaactcccat ccacgccagg
     7561 ccatccagac ggactcggga gtgaatcgtc cttgccggtc agccttcccc ttgagcagtg
     7621 cgagcatgtc cagaagccgg gggtcgtcct ggagccaact gaagcgcgac agcacgtcga
     7681 gcacgtgcag caggtcatac cagaccagcg gggctttgag cttgcggaag tccgtgccca
     7741 tgtagaactg atacggatgc cgggtcgtgc tgtcggccca cagcgtcagc agcgtgttcg
     7801 cccccacgtg gcagggagca gtctcgcgga gcgcggggga cgccgacaag gccttgagca
     7861 tggcaaggtt ggcaaacggg cacgggtcgt ccttgcgtcc cggtccgcgg aaggcgccca
     7921 actccttcga cactgcgcac ggccaaccgt tgtcgcggat cagcgcctcg agatactcga
     7981 tggcggcctg caccacgcgc tgccgcccca acccgaacct ggtcagcgag gagacgatga
     8041 gcggcgtatc gcaaagagcc caggccggct ggtccaggcc cgttcctccg aagtgcgccg
     8101 ggatgttcat gggcacctgg aaggggccgt ctgctgagcg gtgctccagg acgcgcgcca
     8161 cgacctcgtc cacgccagga tcgccgacgc ggagtccgag gtctgcgacg aacgtgagct
     8221 tgtgaagcag gaggccggcg ttcttgtggc ttgatacgac cgaccccggc cactctgcca
     8281 gttccgcgag aacgtgacgc acctggggac tggccagcat cgattctctg gcgtcgagca
     8341 cctcgaggtc gtcttccggc cgccccagca ggtcgcgcct cgtccggtac tcgatccagg
     8401 gatcaccttc gagcagccag tcgatgggca tctccacggc tctatccctc cgtgctgtga
     8461 tgcgtccgag cgtccaacca ggatcggcag tagggagaag gcctccgaca ctgcatctcc
     8521 ttccgcacaa gactctacca tcaggttcgc atggggatct gcagaccgcg tacgaccgag
     8581 gctcccgcac ttgaccaacg tcaggcctgc cggcgtaggg cggggggtat cctgagggtg
     8641 accgaaggcg ttcgggccgg cggccgcgag gcagcccggc ccccttcgag gaggcgagcg
     8701 atgacgatgg acagaggctg cccgggccag gaccggagca actggacgcc ggacgacatc
     8761 ttcgaggcgc cgtgctcgaa ctgcggcgcc ctgatcgagt tcttcaagga cgacgcgaag
     8821 cgttactgcg cggcctgcgg cacctgcaac cccaacccgc gccaggacat cgggtgcgcc
     8881 gcctggtgcg ccgccgccga caagtgctcc acggggcggc agtggctcga ggaggactcg
     8941 ctggccgaca cgcccgcctt cggcgacccg gcctgagcgc agcgtgaggg gccggggcgg
     9001 cgccagcgcc accccggccc ccttggtcgt gccgggcgtc tcccccgccc ggcacgtctg
     9061 agcgcgtccc cccgggcccg ctcaggtctc agagcttcag gtacgagtcc gcgtagaggg
     9121 tctcgttggt gagcatgcgg taggtcttcc agagctcgag ctgctcctgg tcgtccaggt
     9181 cgaccagcgc cgggtcgagc ttgtccatgg cggcgaccgc gtcgtacagc gcgacgacga
     9241 gcttgcccag cggcgtgctc tcgtcgcgct gctcggcgat gcggatccac tcgttctgca
     9301 cgccgtccag cgggtcggcg atcgcgctgt ccagcccagc gcccatggcc atgaccatca
     9361 tgacgcggtt gatgagcggg cggacctccg gcagggcgcc gttggagatg ttggagaggc
     9421 cgatgaccgt ggtcggaggc gggtcggcca tcatcttgaa catgcgcacg gcctcgacgg
     9481 tctccatggc cacgtcctgg ttgcagttca cggccaggac gagcgggtcg aggaagacgt
     9541 cggcgacggg gatgccggcg gcctcggcgg ccgggagcag cgcctccatg gcgatgccgg
     9601 cgcggccgtc cgcggtggtc gggatgccgt ccacgcccat cgtgagcgcg atcaccttgg
     9661 cgccgtacct ggcggcgagc tgcatcacgc tgtcgagccg gtccgggtcg gcggacgccg
     9721 agttcagcat ggcgggctgc ttgcagacct tgaggcccgc ctccatggcc gccagattcg
     9781 tggtgtcgag cgagagcggg gtgtccacga cctcctgcac cgcctggacg atccacggca
     9841 tgacctcgtg gccgagcttc ttctgcgggc cgatgttgag gtcgacgatg ccggcgccgg
     9901 cctcgacctg ggcgaccgcc atggcctgga tcgtgccggc gtcgcgggcc tcgatggcgg
     9961 ccttgaccgg cggcgcgatg atgtggatgt tctcaccgat gatgagcacc ggcggcctcc
    10021 ttctcctggg gggtggcgag cgcggcggcg acgacaggtt cgagcgccgt gctcaacagg
    10081 ctgtcggccg gcaggccgac gagcggtctg ccggcggcgt cgaactcggc gacggccggg
    10141 tcgtccggca ggacggcgag cagctcgagg ccgtgctcgg cgatcgccgc ctcgagctcg
    10201 ggcgacaagc ctgaggcggc acggttcacg atcaggtagt gccgccccac cttggtccgg
    10261 agctctccca gcagcgtggc gacccgaccg gcggtcgtga cgccgcggat ggtcgcgtcg
    10321 ctgacgatga agagcacgtc cacgtcgcgg gtcgtgcgcc gggagagatg ctccagtccg
    10381 gcctcgttgt cgatcacgac ccacggatag ctgtcggcga tcttgtcgat gatcaggcgc
    10441 agcatgttgt tggcggcgca gtagcagccg ggtccctcgg gccggcccat cgcgagcagg
    10501 tcgacgttga cgccctcgac caggctcgag ttgatctcgt agtcgagcag gtcgttcttc
    10561 gcgatgcccg cctccagcag gttggcgcgg gccttctcgc tggtctcctc gcgcacgtcg
    10621 ccgacggtct tctccagggg gaggtcgagg accgtgttga ggttgctcgc gggatcggcg
    10681 tcgatggcga ggatggggcc ggcctgtact tccgccagcc ggcggatgag caggccggcg
    10741 agcgtcgtct tcccggtccc ccccttgccg gcgacggcga tcgtggtggt catggatcag
    10801 cctccgtttc cgctcccgtg gctccgctcg gccagagccg cgcggacgct cgggaacccg
    10861 gtgaggtcgg tgtgcgggag gaagagcgcc gacgtgtact cgtccatgaa cgtgttgtcg
    10921 gcgctcagct ccaggtacgt catcttgctc gccacgtcgg cgacctcgcg gcggacgttg
    10981 acgcagagca ga
//
LOCUS       DASUMX010000152        26669 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1772874, whole genome shotgun sequence.
ACCESSION   DASUMX010000152 DASUMX010000000
VERSION     DASUMX010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 26669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26669
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1772874"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="VFZ86_07240"
     CDS             <1..484
                     /locus_tag="VFZ86_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5642120.1"
                     /translation="LRGERLADFRREYLGFVFQSFNLVPYLTALENVTLPLAVKKMPA
                     AVKRARALDVLERVGLADRGGHLPGKLSGGEQERVAIARALVNEPPLILADEPTGALD
                     SETTEEIMELFARLHREGNTIVMVTHNPEIQRYFDRTIVLRDGRLDCELPACASGTPV
                     "
     gene            518..1483
                     /locus_tag="VFZ86_07245"
     CDS             518..1483
                     /locus_tag="VFZ86_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642121.1"
                     /translation="MLARLSDARGGAPAHAGSRGRPLRLPAVAAAVLALVILLPSAAA
                     ASTGVDDWVHAWEVVRLVDYRQSQPWPPDGIMVYYLGDSIARESTVSDADWSRQLALR
                     AATAGKATAFGFTVAGHNQTFGMDERIVEGLPVTPDRQPRGIVLIGVGISRFIGPPAP
                     LDPAALDPVAPDAEPPLSAWVQHRYEGRAPLSPARKRELAARWMERRWDGFQANRRAN
                     FAAINRLIAACKAKRLRPILFDLPLNFAVVGHRLDEPLSVIRGRCTDIARRNGIKYLR
                     FNRSLGLRSAVFWDLHHLLGPGTTRWQSRLSDELVKLLPDAQPVQ"
     gene            1539..2888
                     /locus_tag="VFZ86_07250"
     CDS             1539..2888
                     /locus_tag="VFZ86_07250"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5642122.1"
                     /translation="MDAQRILRTYYGIAATTTLAQALIWGVNTLFLLSVGFDIFEVML
                     INAAYTVAQVVFEVPTGVVADTVGRRVSYLLAVGTILVSTLLYVGFGLAGYGIWPFVA
                     ASALLGVGYTFYTGAVDAWMVDALQSVGYEGRLEPVFARYGIVFGIFMLIGTTAGGFL
                     GQIELWVPYVLRAVVLVPAFLLGLLVMRDLGFKGRALTLRSFGTETRRIATAGITYGL
                     SDRAVRFIMFASLVQGVFFMYGFYSWQKYFLDLLGQDLVWVTGVIAALVGLTMILGNA
                     VVGRITARVPDRGLILMVCAAVTTVSIIGAALVQQFWVAVPLYLVSTVAFGISLPVKQ
                     GWLNARIPSEQRATIISLDALFGDAGSTAGQVGLGWLSQTVSIPFAWLVGGVVQAAGV
                     PLFGVARRAERAQPAQPAARPSDPGLEVESAGCFGPEPSPTCVPGDDPGTVDGERVA"
     gene            2974..3903
                     /locus_tag="VFZ86_07255"
     CDS             2974..3903
                     /locus_tag="VFZ86_07255"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX5642123.1"
                     /translation="MSTYDIAIIGGGPAGLATAMYAGLRGLGAVVYEAEAFGGQLINL
                     YPTKPVSNFPAQPSLPSRELALRLAEQAEQFGAELREWRAVENVTPQESGFTIDGSLR
                     QDDARTLVLALGLGRFTPRRLGLEDEDRYLGKGLAYRLPPLEEVRAKRAVVVGGGDTA
                     LDTALSLREVADVTVVRRRDEFRAFSHTQDRFAEAGIEVVSNGEVVGLRGKERLESIV
                     VRRPDESSVELPADLVLVSIGQVPDLSGFEHWGLGLSGTRIDVDTTMETRLPGVYAVG
                     DFAAYPGKVRMIATAVAEGSTAAASAQRHLSAA"
     gene            3981..5018
                     /locus_tag="VFZ86_07260"
     CDS             3981..5018
                     /locus_tag="VFZ86_07260"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEX5642124.1"
                     /translation="MSADGALRGASGAGVPVPWAVAAVARGFSLFIGGFTLVSVFGSL
                     RRAHADFTVWWVAVPAVGRVAAGALLVLFSAALLGYAVAPRMRPWRRWLTVGLVLAFA
                     AITAWNGVGFYLAWRAGEMRPGVPVPLSFVVCAALIFVAWAALRPPAPRRRRLAAAVV
                     LVATAAACLLLFPVAQVFFFGETDYRRAADAVVVFGAQVHRDGRPSTSLRDRMTTAVE
                     LYDDGLVRRVIVSGGVGESGFDEALVMRDMAVEAGVPEERVVVDSTGVSTTATVAGTV
                     PFFGEDGETRILAVSQYYHLPRIKLAYQRAGWNVFTVPAGTSSPIPQTPRFVVREIPA
                     FWVYYLQAVFG"
     gene            complement(5142..5774)
                     /locus_tag="VFZ86_07265"
     CDS             complement(5142..5774)
                     /locus_tag="VFZ86_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642125.1"
                     /translation="MDRMTARRRRRRRGLTRLAVLVALIVAIIVVVVLLVRGCGGDAV
                     DEPSGTPPASASAGASAQATEEPEPVSSLPPLVGVGDTVRFETPEGAVVRVTASGYAD
                     PGDAPAGVSADPGERIVTLKLSVTPEGAEGTALVPLPFKKADSFILIAEDDTLTAALT
                     GDALLGATLPPGETISTTLAFSVGASSPIRFLCTPVEGSRPRSATWELGK"
     gene            complement(5952..6869)
                     /locus_tag="VFZ86_07270"
     CDS             complement(5952..6869)
                     /locus_tag="VFZ86_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX5642126.1"
                     /translation="MCAGCGAPIAVRQILMGTEDPVVVSAATGCLEVSTTIYPYTSWK
                     GSYIHTAFENSAATLSGVETAYRALKKKGLVEEDTKFIAFGGDGGTYDIGLQSLSGAM
                     ERGHQMLYVCYDNGAYMNTGFQRSGATPQGAWTTTSPVGKAQSGKIQNRKNLTDIMIA
                     HELGYVAQASPHDPRDLVRKSAKALDTPGATFLNVLAPCPRGWRADGAETIDLAREAV
                     ETCYWPLFEVVDGEYHLTYRPHHKKPLIPWLKRQGRFAHLFKPGNEESLLNLEAWVDN
                     EWEKLLRKCGEPSEAEWQEYLQANGCLLR"
     gene            complement(6966..9215)
                     /locus_tag="VFZ86_07275"
                     /pseudo
     CDS             complement(6966..9215)
                     /locus_tag="VFZ86_07275"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            complement(9128..11044)
                     /locus_tag="VFZ86_07280"
     CDS             complement(9128..11044)
                     /locus_tag="VFZ86_07280"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEX5642127.1"
                     /translation="MTPLAKPSTRIGKALKAAADKSAYLADDKKAEIRVCAGTACHAS
                     GRLALRKALDKALAERGLTDTVTVVETGCHGFCEEGPIVVVRPQGLFYPRLKPKDIAE
                     IIETSVVGDGIVERLLYKDPASGEPLAHEKDIPFYALQDRIVLALNGKIDPYSVDDYL
                     NHGGYTALAKVLEEGDPMGVIDEVEASGLRGRGGAGFPTGRKWRFCRGNPGDVHYVIC
                     NADEGDPGAFMDRSVLEGNPHSVIEGMTIAAYAIGAGSGPVEGYVYVRHEYPFAVERL
                     RAALAQARERGLLGENILGSGFSFDIRINQGAGAFVCGESTALTASIEGHRGMPRGKH
                     IRTVAQGLWGQPTNLNNVESYANVPWIVNHGAQAFAAKGTETSKGTKIFSLTGKVVNG
                     GLIEVPMGATLRHVIFDIGGGMLPGREFKAVQLGGPSGGCLPASLLDDPIDFESLVAA
                     GSMMGSGGMVVVDDSTCMVDFVKFFLKFTAEESCGKCVPCRVGSQRLLEIIERICDGL
                     GTLDDLERLETLSEDIIEGSLCQLGGSAPNPVLSTLRYFREEIEAHIVEHRCPARVCR
                     PLIKYTIDPDACTGCHVCFGACPTQAISGERKEAHVIDQKLCIKCDTCRQVCKFDAIP
                     VVDAQPALAEGRQQ"
     gene            complement(11041..11862)
                     /locus_tag="VFZ86_07285"
     CDS             complement(11041..11862)
                     /locus_tag="VFZ86_07285"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEX5642128.1"
                     /translation="MPRRNGTTATEPKGSALYEVLKGIDFERRELVHKPRGVGLFDLV
                     KDKGGAYKCYACTLCERTCPVDCIEIDYCPEFEELPFDVEAERAAALATLPGESCGAN
                     AACVMGEQPAGFMPEIDMKLFDAVIAGIRGGSGLVQALHSTQEAYGFLPRVALENIAE
                     EMRLPFSRVYGVATFYGQFRLAPVGKYIIDVCMGTACHVAGAPLVVEAFSQELEVPVG
                     ATTPDGLFTLQTVNCVGACALAPVVRLGEDETIGRVGPNEARKLVRKLRKAEVPA"
     gene            complement(11873..13075)
                     /gene="porA"
                     /locus_tag="VFZ86_07290"
     CDS             complement(11873..13075)
                     /gene="porA"
                     /locus_tag="VFZ86_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HEX5642129.1"
                     /translation="MASTQSEILAVTGNEAVAEAMGQIDPDVVCAYPITPQTEVVQLY
                     AQMVADGKVGTEFVTVESEHSAMSAVIGSAAGGARTMTATSSQGFALMWEVLFVAASY
                     RLPVVMTTVNRALSGPINIHCDHSDTMGGRDTGWIQLYGENHQEAYDNCIQAIRIAEH
                     LDVRLPVMHMYDGYVISGAIGPLQMLTKEQVQTFIGPYRPTNPMLDAKNPVTVGPFDG
                     LHGWYFEHKVAQNRAMDRSFKVIQDVADEYAELSGRKYGLLDPYRLDDAEIAIVVVGS
                     TAGTTRMTVDKLRARGVKAGMVRVRAFRPFPHEEYARALEHVKVVGVMDRADSFGAQG
                     GPVFLEVRSALYDCDPRPQVLPFIYGLGGRDIFPDNIEQAFAELENAAQSTKRASTER
                     RYLNLRKD"
     gene            complement(13078..13467)
                     /locus_tag="VFZ86_07295"
     CDS             complement(13078..13467)
                     /locus_tag="VFZ86_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX5642130.1"
                     /translation="MTVDKKAGAKKPAPLKKPAARKAPAKKKPTWDISDVDQWGPERH
                     ELGATIVEAGNSVYNETGGWRSLVPEIDVEKCDGCMLCYFYCPDASILVEDNKAVGID
                     LEHCKGCGICARECPTKAIVMLIDKKD"
     gene            complement(13464..14072)
                     /locus_tag="VFZ86_07300"
     CDS             complement(13464..14072)
                     /locus_tag="VFZ86_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HEX5642131.1"
                     /translation="MATKTGKTNVGAPPSLTEIRWHGRGGQGAVTAAKMVAELALGEG
                     KYFQAFPEYGPERSGAPIVAFTRVSDAPIQVYSGIEHPQIVVVLDSSLLKIVDVTKGA
                     PDDAVVLVNSERSPAQLRKDSGLVGNRLFTIAATRIAVETIKRPIPNTPMVGALTRIT
                     GLFDIDDVVTFLREDFGKKFPPKVVEGNIAAITRSYEEVQGE"
     gene            complement(14075..14677)
                     /gene="recR"
                     /locus_tag="VFZ86_07305"
     CDS             complement(14075..14677)
                     /gene="recR"
                     /locus_tag="VFZ86_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009740947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEX5642132.1"
                     /translation="MYAPPVERLISELSKLPGIGPRTAQRLTFHILRQRPEEVLPLAA
                     AIAEVKEKIGFCSRCFNLAEGELCTICADPRRDPRLLCVVEQPFDVVTIERTREYHGY
                     YHVLGGALSPIDGVDPEHLHMAELLARVDEDVAEVIVATNPTMTGEATAMYVADLLPE
                     RVRVTRLASGLPVGGDLEYADELTLGRALAGRRDVRHGEE"
     gene            complement(15205..15507)
                     /locus_tag="VFZ86_07310"
     CDS             complement(15205..15507)
                     /locus_tag="VFZ86_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003349813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEX5642133.1"
                     /translation="MMKQVQEMQRKMAQAQEELAVETVEASAGGGVVTVVVTGSLEVK
                     EVRINPDAVDPEDVEMLQDLVVAATNEALRAAQELASTKLGGATGGIDLPNIPGLF"
     gene            complement(15542..17446)
                     /gene="dnaX"
                     /locus_tag="VFZ86_07315"
     CDS             complement(15542..17446)
                     /gene="dnaX"
                     /locus_tag="VFZ86_07315"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEX5642134.1"
                     /translation="MKRSLYREYRPQTFSELVGQDHVARTLRNAVETGQVAHAYIFAG
                     PRGTGKTSTARILAKTLNCVGADGANDSPTITPCGVCRHCVAIADGVSLDVVEMDAAS
                     NRSIDDVRDLRDKIAFAPVDGRFKVYIIDEVHMLTREAFNALLKTLEEPPDNVVFVLA
                     TTEAHKIPETIVSRCQRFDFHRPQVAGIVRLLAGIVERENARPDDERGGPPIDIQEEA
                     LHIIARHSQGGFRDAIGTLDKLVTYAEGTITSADVLEALGVTSSDLLFEITDIVTERR
                     TAEALQFVQRLANEGTDYSQFIRDLLRHLRQLFLLQHLEEAASDEAMLRTLGQTVELD
                     GELFREQAQRAGQVSPREVVFFIEQLGEAQREIRDGLDPRLQLELALVKVTRPDLDHS
                     EAALEERLRRLEAAATGGGPAAPAPPAAAGPASSAPAPAPARPATDAAAPARPPSDAA
                     ATAPATPPATTPGSPAVPAKPQPADVAPPGPAPAEAQPDPAAAPPRPATAADAAQELT
                     LERVKRAWELILQRVQSARVPLYGFLRDGRPHALEGDALTVALPSEFALRNAEQGDNA
                     QVLATAVEGVLGRRVTARFELASGGGPAATPEAAPAAAPQAPAALPDFTDQIRAAQAK
                     LDAELLPEEP"
     gene            complement(17475..18944)
                     /locus_tag="VFZ86_07320"
     CDS             complement(17475..18944)
                     /locus_tag="VFZ86_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HEX5642135.1"
                     /translation="MNQPADQHRELLARVAREAMIEYGLEPEFPARAIAEAGALPEAS
                     ADGEGAADLRALLWCSIDNDDSLDLDQLTVAEGEVDGGVRVLVAIADVSATVLPGSAV
                     DAHAGVNTTSVYTPGRIFPMLPDRLSTDLTSLNPAEDRLAVVVGMTVDAGGAVVAEDV
                     RRALVHNHAKLAYPSVGAWLQGEGGMPPALAAVEGLAENLRLQHTVAQRLRARRREQG
                     ALDLETLEVKARFDGGAVSGLVVEPRDRAKELIEDFMIAANGVVARYLAGKRFPVVRR
                     VVREPERWQRIVDLADQHGFLLPEEPDAPSLNAFLEEERESDPERFPDLSLAIVKLLG
                     SGEYEATQPGEPIDGHFGLAVTHYAHSTAPNRRYPDLITQRLVKAALDGSPIPYSGER
                     LAELAAHCTHQEDQAQKVERRVRKSAAACFLVERVGDEFDGFVTGASPKGTWVRALDP
                     PVEGRVVEGAGELDVGDAVRVRLVDADPERGFIDFAVIS"
     gene            complement(18941..19768)
                     /locus_tag="VFZ86_07325"
     CDS             complement(18941..19768)
                     /locus_tag="VFZ86_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEX5642136.1"
                     /translation="MKLPPDPASPADRDPLAYQDAAFVESDEGRPLRILAEYLRPLGV
                     FRRRRIHDTIVFFGSARITEDGPLGRYYTEARDLARLITEWSDGLTSPAHRYLVCTGG
                     GPGIMEAANRGAHEAGGRTIGLNIGLPHEQRPNPYITEGLSFEFRYFFMRKLWFAYVA
                     RAIIVFPGGYGTLDELFEFLTLSQTGKIDPPVATLLYGTRYWNEIVDFDALVRHGTIS
                     ADDLRLLHFVDTPAEAMAVLRARLRPQDEAEPAPALARSVTPEDECATCGPEKEERP"
     gene            complement(19808..20073)
                     /gene="ffs"
                     /locus_tag="VFZ86_07330"
     ncRNA           complement(19808..20073)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VFZ86_07330"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(20085..20996)
                     /locus_tag="VFZ86_07335"
     CDS             complement(20085..20996)
                     /locus_tag="VFZ86_07335"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5642137.1"
                     /translation="MGSFAKAGGWRGPAAPGDFVVAGGVRVHYVRGGAGPPVVYVHGA
                     KGSVYDFTLSVGERLAERYTALAMDRPGSGFSARPANGGNTPQAQAAVLRAAASELGL
                     RRPILIGHSLGAAVALAWALAAPDEVAAVVTLCGYVLPLGGGPPWVTTLLRHPATLRG
                     VGLLGRSRLGRPLVRSAVERAFSPGTPPDEYVRIAPQLALQASALISDGEDRKAVESG
                     LAALRPLYPGLRAPLVILVGAQDRIVPPAVSDRLHALVPGSELVRVAGAGHMPQFTAP
                     DAVVAAVDRAAELAGAAPTFASPDDHG"
     gene            21058..23364
                     /locus_tag="VFZ86_07340"
     CDS             21058..23364
                     /locus_tag="VFZ86_07340"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX5642138.1"
                     /translation="MGTRRYLFAGLIVGLVLAVVMVAITPFGAPRAAPSSAPAGRFSS
                     ARAVDHLRIVAGGSRPIGSAGAAAAAGYIRRHLESLGLDPHTQTAKVVSAIDPRVAGM
                     VHNVVGRLPGTDPSRAVLLMTHYDSVPTSVGAADDGSGVAALLETARALRSGPPPRND
                     VIFLFTDGEEQGLLGARAFLRDDPWAYAVGVAFDFDSPGSSSPALMYETSPGNGLLVR
                     EYLAATDPFSSSLMYEVSRRQRIVGDFQLFLARGIPGMSFGMLDGPGYYHTAYDSLAS
                     FDEAALQHEGETALSLARRFGALDLWHVRRPDVVYFNVIGDVAVVYARSLVAPFAALA
                     VLLFAAAVGTAAARRLLSLRGLAAAALGTGATLVASLLVTAVSWWMYESAYQERIWTR
                     TGVVISDWYRVGLVLVVAAVVLGIYGFLLRRLHAWDLAVAALAWWCAGAVGVSLLFPG
                     ASFLLTWPLVGGSLGLIGAALIERPSGDRPAAALIALAGAVPGLVLLSSAVYLLLMSA
                     GLKQGVTLVAVWLLAGLLMLPLAVVERAFRFWPPIVLAVTGGIVLFAVGSTVAFDAEH
                     PKFTSVSYRVQADGTPVWQAIGRLDDYTRAYVHPRPGAPPVQSYFPDLGVRDTVVGEA
                     PYYGLTAPRLRMLSDHVEGDRRTVRVRLRPTRDAAVVSLLVHTVVGNLTASVDGHELG
                     GRDTTLLDVTTVRWWFDFYAPPPEGVVVTLDFVAGPRVLLRAVDFAHGLPAGAAGTYP
                     ARPAGMLPGGIGDGSFTETELRLPRVAP"
     gene            complement(23371..26256)
                     /locus_tag="VFZ86_07345"
     CDS             complement(23371..26256)
                     /locus_tag="VFZ86_07345"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5642139.1"
                     /translation="MRPKRVNRAPASVKLHKKLEPDTQRAEQLTAEAPQSRSDSPRGS
                     PAGGSSWTHDAAEQSNHERRDQQMSTRNLDKLFDPKSIAVIGASNKKGSVGYILLRNL
                     IGAEYQGIVYPVNVSAQSVQGIQAYASISQVPRKIDLAIIAVPAKNVPQTMRECGEAG
                     VAGAVIVSSGFKEIGKAGKRLEDEVKSIAESYGVRIVGPNCLGYIRPAMNLNATFAHV
                     IPPAGRVAFFSQSGALGTAILDWAAANNIGFSAFVSVGSMSDVDFGDLIDYFGADAHT
                     SSIILYIESITDARKFMSAARHFAKSKPIIVVKSGRTARSALAAASHTGAIAGDDTLY
                     SAVFRRAGVVRVDQIEDLFDASEALSRVSSPRGPHLGIVTNAGGPGVMACDRLLHLGG
                     ELAELAPETDVKLQDALPGFAARGNPVDVAGDADAVRYATAAQALMDDPNCDGVLAIL
                     TPQAMSDPTGTALALIDVAHSHQLKPLLTSFMGEIKVAEALRILRDAHVPTFGTPEDA
                     VGAYMYMYQYTRNLGNLYETPADILPDFEPNRDAVKRIFLEVARDGRSILSEPEAKAV
                     LEAYQIHAVRTLVAKTAEECAKAAEEIGFPVAIKILSHDITHKSDVGGIALNVRSGPE
                     AANQFVKITDRVREAQPDATIIGVAVQEMSRGGYEVILGSKKDPTFGPAMMFGMGGTG
                     VELYRDVAVDFPPVNQALAQSMIQSTKVSQLLRGYRGKAPVDMVSLEQALVKMSYLLV
                     DFPEILEMDINPLQVRTDGVCALDARLVIEPKDVRKIALPGAHLMISMYPSKYRWEVP
                     TDGEDVVIRAIKPEDEPLWADMIESLSPATAEYRFFGPIKEVTKSMLVRYCHIDYDRE
                     IALAAIREPKGKKKKMMLGVARLTIETANVEEGEFAIVVRDAYQRKGIGSKLMDALIQ
                     AARDRHVREIRGHVLAANPGMTRFAEDLGFDVRPGDEPEVRELVLRL"
     gene            26328..>26669
                     /locus_tag="VFZ86_07350"
     CDS             26328..>26669
                     /locus_tag="VFZ86_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642140.1"
                     /translation="MTEPIYAQRYRSKKRLPAGRAAAAYAGEDAQGRPVVVTIVRPLD
                     PDVFLRSVGVVRSVRHLDLAAVVDAGREGPDCYVVAEDPGGSDAATLATRGPLPVPGA
                     ALAAASAAAGLA"
BASE COUNT         3335 a         9404 c         9702 g         4228 t
ORIGIN      
        1 gcttcgcggc gagcggctcg cggacttccg ccgcgagtac ctcggtttcg tgttccagtc
       61 cttcaacctc gtgccctacc tgacggcgct cgagaacgtc acgctgccgt tggcggtgaa
      121 gaagatgccg gccgccgtga agcgtgcccg cgcgctggac gtgctggagc gcgtgggcct
      181 cgccgaccgc ggcggccacc tgcccggcaa gctgtccggc ggggagcagg aacgcgtggc
      241 catcgcccgc gcgctcgtca acgaaccgcc gctcatcctc gccgacgagc ccaccggcgc
      301 cctcgacagc gagaccacgg aggagatcat ggagctcttc gcgcgactcc accgcgaggg
      361 caacacgatc gtgatggtca cgcacaaccc ggagatccag cggtacttcg accgcacgat
      421 cgtgctccgt gacggccggc tggattgcga gctgccggcc tgtgcctccg gcacccctgt
      481 ctgaccgcct ccgggcgctg cggtacacta cgaccctatg ctcgctcgac tctcagatgc
      541 gcgaggtgga gcccctgcgc acgcagggtc ccgcggccgg ccgctgcgcc tgccggcggt
      601 cgccgccgcc gttctcgcgc tcgtcatcct gcttccgtcc gcggcggcgg cctccaccgg
      661 cgtcgacgac tgggtccacg cctgggaggt cgtgcgcctc gtcgactacc ggcagagcca
      721 gccctggccg ccggacggca tcatggtcta ctacctgggc gactccatcg cgcgcgagtc
      781 cacggtgagc gacgcggact ggtcgaggca gctggcgctg cgcgcggcga cggccggcaa
      841 ggcgaccgcg ttcggcttca ccgtcgccgg ccacaaccag acgttcggta tggacgagcg
      901 catcgtcgag gggctgccgg tgacgccgga ccggcagccg agaggcatcg tcctcatcgg
      961 tgtgggcatc agccggttca tcggcccgcc ggcacccctg gaccccgcgg cgctcgaccc
     1021 ggtcgcgccg gacgccgagc cgccgctcag tgcgtgggtc cagcaccgct acgaagggcg
     1081 cgcgccgctg tcgccggcgc gcaagcgcga gctcgccgcc cgctggatgg agcggcgctg
     1141 ggacgggttc caggccaacc gccgcgccaa cttcgccgcg atcaaccgtc tgatcgcggc
     1201 ctgtaaggcc aaacgactgc gcccgatcct gttcgacctg ccgctcaatt tcgccgtcgt
     1261 cggtcaccgc ctcgacgagc cgctgtcggt catccgcggg cggtgcaccg acatcgcccg
     1321 ccgcaacggc atcaagtacc tgcgcttcaa ccgctccctc gggctccgga gcgccgtgtt
     1381 ctgggacctg caccaccttc tcggtcccgg aaccacccgc tggcagtccc gtctttcgga
     1441 cgaactggtc aagctgctgc cggacgctca gcccgtccag tagggccggc catcggtcgc
     1501 ggcgcgttgc ccggccgcgc ggcgggaaca agccgcccat ggacgcgcag cgcatcctcc
     1561 gcacgtacta cggcatcgcc gccacgacga ccctcgcgca ggcgctgatc tggggcgtca
     1621 acacgctgtt cctgctctcg gtgggcttcg acatcttcga ggtcatgctc atcaacgccg
     1681 cctacaccgt cgcgcaggtg gtgttcgagg tgcccacggg cgtcgtcgcc gacacggtcg
     1741 gccgccgcgt ctcgtacctg ctcgcggtcg gcaccatcct cgtctcgacg ctgctctacg
     1801 tgggcttcgg cctggccggc tacggcatct ggccgttcgt cgcggcctcc gccctgctcg
     1861 gcgtcggcta caccttctac accggcgccg tcgacgcctg gatggtcgac gccctccaat
     1921 cggtcggcta cgagggacgc ctggagcccg tcttcgcccg ctacgggatc gtgttcggca
     1981 tcttcatgct gatcggcacg acggcgggcg gctttctcgg ccagatcgaa ctctgggtgc
     2041 cgtacgtgct gcgcgccgtg gtgctggtgc cggccttcct gctcggcctg ctggtgatgc
     2101 gcgacctcgg cttcaagggg cgcgccctca cgctgcgcag cttcggcacc gagacgcggc
     2161 gcatcgccac cgcggggatc acctacggcc tcagcgaccg cgccgtgcgc ttcatcatgt
     2221 tcgcctcgct cgtgcagggc gtgttcttca tgtacggctt ctactcgtgg cagaagtact
     2281 tcctcgatct gctcggccag gacctggtct gggtgaccgg cgtgatcgcg gcgctggtgg
     2341 gcctgacgat gatcctgggg aacgccgtcg tcgggcgcat caccgcgcgg gtcccggacc
     2401 gcggcctcat cctcatggtg tgcgccgcgg tcaccacggt gagcatcatc ggcgccgcgc
     2461 tggtgcagca gttctgggtc gccgtgccgc tctacctcgt ctccacggtg gccttcggca
     2521 tctcgctgcc ggtcaagcag ggctggctca acgcgcgcat cccgtccgag cagcgcgcga
     2581 ccatcatctc gctcgacgca ctgttcggcg atgcaggctc cacggcgggg caggtcggcc
     2641 tcggctggct ctcccagacc gtgtcgatcc cgttcgcctg gctggtcggc ggagtcgtcc
     2701 aggccgccgg cgtgccgctg ttcggcgtgg cgcggcgggc ggagcgggcg cagcccgcgc
     2761 agcctgccgc gcgaccgagc gaccccggcc tcgaggtcga gtcggccggc tgcttcggcc
     2821 cggagccgtc acccacctgc gtccccggcg acgatcccgg gacggtggac ggcgaacgcg
     2881 tcgcctgacg cctgcggcac gtgccgcggt ttcgtgtgtg attacgctca gaacgtggaa
     2941 cgctcgcact gtcaccctga gagggagccg gtcatgagca cctacgacat cgccatcatc
     3001 ggtggagggc cggccggtct ggccaccgcc atgtacgccg gcctgcgcgg cctcggcgcc
     3061 gtcgtctacg aggccgaggc gttcgggggc cagctcatca acctgtatcc gaccaagccc
     3121 gtcagcaact tcccggcgca gccgtcgctg ccctcgcggg agctcgcgct gcgcctcgcc
     3181 gagcaggcgg agcagttcgg cgccgagctc cgcgagtggc gcgccgtcga gaacgtgacg
     3241 ccgcaggaga gcgggttcac catcgacggc tcactgcgcc aggacgacgc caggacgctg
     3301 gtgctggccc tgggcctggg ccgcttcacc ccgcggcgcc tcggcctcga ggacgaggac
     3361 cgctatctcg gcaagggact ggcgtaccgg ctgccgccgc tcgaggaggt ccgcgcgaag
     3421 cgcgccgtcg tcgtgggtgg cggcgacacg gctctggaca cggcgctctc gctgcgcgag
     3481 gtcgccgacg tcaccgtggt ccgccggcgt gacgagttcc gcgccttctc ccacacccag
     3541 gaccgcttcg ctgaggcggg catagaggtc gtcagcaacg gtgaggtggt cggcctgagg
     3601 ggcaaggaac ggctcgagag tatcgtcgtg cggcgtccgg acgagtcctc ggtcgagctg
     3661 cccgccgacc tcgtgctggt cagcatcggc caggtccccg atctctccgg cttcgagcac
     3721 tggggcctcg ggctcagcgg caccaggatc gacgtcgaca cgaccatgga gacgcggctg
     3781 cccggcgtgt acgcggtcgg cgacttcgcc gcctaccccg gcaaggtccg catgatcgcc
     3841 accgccgtgg ccgaaggttc gaccgcggct gcctcggcgc agcgtcacct gtcggcggcc
     3901 tgaccggggg gtggcccgac ggaggcggcc ggcgtccggt cccccggccc acggacgtcc
     3961 gaggtgatac agtcccgccc atgagcgcgg acggagcact gcgcggggcc tccggcgccg
     4021 gtgtgccggt cccttgggct gtggccgccg tcgcccgggg tttctccctc ttcatcggcg
     4081 gcttcacgct cgtcagcgtc ttcggctcgt tgcgccgcgc tcacgccgac ttcaccgtct
     4141 ggtgggtcgc cgtgccggcc gtggggcgcg tcgcggccgg cgcgctcctg gtgctgttct
     4201 ccgcggcgct tctcggctac gccgtggcgc cgcggatgcg gccgtggcgg cgctggctca
     4261 ccgtcggtct cgtccttgcc ttcgccgcca tcaccgcctg gaacggcgtg ggcttctacc
     4321 tcgcctggcg cgccggggag atgcggcccg gcgtgcccgt cccgctctcc ttcgtcgtct
     4381 gcgccgcgct catcttcgtc gcgtgggccg cgctccggcc gccggcgccg cggcgccggc
     4441 ggctcgccgc ggccgtcgtc ctcgtggcca ccgcggccgc ctgcctgctg ttgttcccgg
     4501 tcgcgcaggt cttcttcttc ggtgagaccg actaccgccg cgccgccgac gccgtcgtcg
     4561 tcttcggcgc gcaggtgcac cgcgacggac gcccgtccac ttcactcagg gaccgcatga
     4621 ccacggccgt ggagctctac gacgacggcc tcgtcaggcg agtcatcgtc tcgggcgggg
     4681 tgggggagag cggcttcgac gaggcgctcg tgatgcgcga catggccgtc gaggccggtg
     4741 tcccggagga acgcgtggtg gtcgattcca ccggcgtcag caccaccgcg acggtggccg
     4801 gcaccgtgcc gttcttcggc gaggacggcg agacgcgcat cctcgccgtg agccagtact
     4861 accacctgcc gcgcatcaag ctcgcctacc agcgcgcggg ctggaacgtc ttcacggtac
     4921 cggccggtac gtcctcgccg atcccgcaga caccgcgctt cgtggtacgc gagatccccg
     4981 ccttctgggt gtactacctg caggccgtct tcggctgagg tacggcaggg catcgcggcg
     5041 aggagtgacg tcaccggtga tgtcatccgt ggcgccacgg gtgctgccgt ccgcggcgtc
     5101 gcacgcggcg cagccaccgg cagccggttc cggcgcgcct gctacttgcc cagctcccag
     5161 gtggccgagc gcggccgtga cccctccacc ggcgtgcaca ggaagcggat cgggctggac
     5221 gccccgacgg agaacgcgag cgtggtgctg atggtctccc ccggcgggag cgtcgccccg
     5281 aggagtgcgt cgccggtgag cgccgccgtc agcgtgtcgt cctcggcgat gaggataaac
     5341 gagtccgcct tcttgaaggg caggggcacg agggcggtgc cctcggcgcc ctcgggggtc
     5401 acgctcagct tgagagtgac gatgcgctcg ccggggtccg ccgagacgcc ggccggcgcg
     5461 tcgccggggt ccgcgtagcc gcttgcggtc acgcgtacca cggctccctc cggcgtctcg
     5521 aaccgcaccg tgtcgcctac gcccaccagc gggggcaggc tggagaccgg ctccggctct
     5581 tccgtggctt gcgccgacgc ccccgcgctc gccgaggcgg gcggcgtccc ggagggctcg
     5641 tccaccgcgt ctccgccgca gccgcgcacg agcagcacca cgacgacgat gatggcgacg
     5701 atcagggcga cgaggacggc gaggcgtgtg aggcctcggc ggcggcgtcg ccggcgtgcg
     5761 gtcatgcggt ccacggtgct cctcccggcc gggtcgggtg aacggcggcc atgtgattgt
     5821 actcgccggc gcggaagcgc cggaacgcga aggaccgggg cgccggggag gaggcgccga
     5881 acggcgaagg gccggggcgc ctgtcggcgc cccggccctt cgctctgctt cggctggtag
     5941 ccggctgcag tctaccgcag caggcagccg ttggcctgca ggtactcctg ccactcggcc
     6001 tcgctgggct cgccgcactt gcgcagcagc ttctcccact cgttgtccac ccaggcctcg
     6061 aggttgagca gagactcctc gttgccgggc ttgaacaggt gagcgaagcg cccctggcgc
     6121 ttgagccacg ggatgagcgg cttcttgtgg tgcggccggt aggtcaggtg gtactcgccg
     6181 tccacgacct cgaagagcgg ccagtagcag gtctccacgg cctcgcgggc gaggtcgatc
     6241 gtctccgcgc cgtccgcgcg ccagccgcgg gggcagggcg ccagcacgtt gaggaacgtg
     6301 gcgcccggcg tgtccagggc cttcgccgac ttgcgcacga ggtcgcgcgg gtcgtgcggc
     6361 gaggcctggg ccacgtagcc cagttcgtgg gcgatcatga tgtcggtgag gttcttgcgg
     6421 ttctggatct tgcccgactg ggccttgccc accgggctgg tggtcgtcca ggcgccctgc
     6481 ggcgtggcgc ccgagcgctg gaagccggtg ttcatgtagg cgccgttgtc gtagcagacg
     6541 tacagcatct ggtggccgcg ctccatggcg ccggagagcg actgcaggcc gatgtcgtag
     6601 gtgccgccgt cgccgccgaa ggcgatgaac ttggtgtcct cctcgacgag gcccttcttc
     6661 ttgagggcgc ggtacgcggt ctcgacgccc gagagcgtcg cggccgagtt ctcgaaggcg
     6721 gtgtggatgt agctgccctt ccacgaggtg tacgggtaga tggtggtgct cacctcgagg
     6781 cagccggtgg ccgccgagac gacgaccggg tcttccgtgc ccatcaggat ctggcgcacg
     6841 gcgatcggcg ccccgcagcc ggcgcacatg cggtgaccgc cggccagctc gctgcccttg
     6901 gccacgagct cgcgccactt ggtgcgcgcg tcctcgaagt ggttggtgtc cttgagcgcg
     6961 tccaccgggc acacgtccac gcactcgtgg cacatgatgc agcggctctc gtcgaccctg
     7021 aacgaggcga ggtcgcgctc gttgaaggtg agcgccccgg tggggcacga cgtgacgcag
     7081 cggccgcagc tgatgcagtc ggcgagctgc agcgcgtcgc tgtagggggt gtccacgttg
     7141 atggtgaagc cccggccggt gaagtcgtag caggccgggc cggcgacgtc gcggcacacg
     7201 cgcacgcact tgccgcaggc gatgcagcgg ctcatgtcgc ggcggatgaa cgggtgctcg
     7261 tactgcggct cgacctcgcg caccaggttg cccttgacga cgaggtcgtt gtcggtgatc
     7321 tcgtactgga cgccgagcgt ctgcaggtcg caggcgccgt tgctcgcgca ctcgcactgc
     7381 aggcagcggt cggcctcggc gcgtgcggcg gcgaggctgt agcccttctc gacctcgagg
     7441 tcgccggccg tgaggcgctt ctgcctgccg accttgggca tcgtggcgcg aggcgccggc
     7501 tcggcggtcg cgcccatctt gagccacacg gggctgcgct cggcgagacg cgcgccgagc
     7561 tcgccgagct gctcctcgtc cttgagctgg tcgaggtacg gcatgccgtt gaacacggcg
     7621 agacgctcct cgagctcctc gaggtcctca ccgcgcagcc aggcgtcgac ggcgagcgcc
     7681 gagcgcttgc cgctcgccac cgcgtggatc acggagcgcg agccggtgat ggcgtcgccg
     7741 gcggcgaaca ggccttcttc cggcgtgcgc ccggtgtagt agttggcgat caggcgcgag
     7801 tcggccatgg ccaggtagtc ggcgctgtcg ccgagcttgg gagcgtaccc ggtggccatg
     7861 acgacggtcg tcgcggcgag ctcgaagcgc gagccgcgga cctccttgcg gcggcccttc
     7921 tcgtacgcga cgcggacgca ctcgacgccc tgcgtcttgc cgcccttggc cttgatcttc
     7981 ttcgccaggg cgccgaactc gaccctgacg ccctcctcga gggcggcggc caggtcacgg
     8041 ccgccggcgg ggatgtcgtc ggcctggtgc tgggcgatga cgacgacgtc cttggcgccc
     8101 ttgcggcgcg ccgtgcgcgc gacgtcgaag gcggtgatgc cgtcgccgat caccgcgacc
     8161 ttctgcgaga acttgatggc ccggccttca cgggcgcgct tgagcagatc gaggccgttc
     8221 agggcggcgt cgtcccccgg gaggacgtgg tcctgctcct cccagttgcc gacgctgatg
     8281 atcaccgcgt cgaagccggc gtcgaacagg ccgtccgggt cgacctcgta gccgagctcg
     8341 gactcgccga cgaagcgcac gccggcgtcc cagagcgggg cgagctcctt ctcgacgacc
     8401 ttcccgggca ggcggaactc ggggatgctg tagcgcagcg tccctcccgg ctcctcgttg
     8461 gcgtcgtaga gggtcacctc gtggccgccc tgggtgagga accaggccgc ggcgagcccg
     8521 gccgggccgg cgccgatcac ggcgacccgc cggccggtcg gcacgcccgg gatcagcagc
     8581 gtctggctgt ggtcggccgc cgcccggtgc agggcgcaga tggcgatggg ctcgtccacg
     8641 tcgccacgcc ggcagaccgg ctcgcagtag cgcgggcaca cgcgcccgag gatgccgggg
     8701 aacggcagct gctcgcgcac gatggtggcc gcgccggcgg cgtcgccgga ggagagcgcc
     8761 gccatgtagg ccgggatgtc gatgtgcgtg gggcacgcgt gcgagcacgg cgcctcgcag
     8821 taggcgttgt ggtccgagag gtacatcttg aggtagctct cgcggcgcgc gcggacgcgc
     8881 ttggtgtcgg tcttgacgac ctggccgtcc ttgacccacg tggtgcaggc cgcctggagc
     8941 ttgtcgaggc cttcgacctc gaccaggcac aggcggcagg cgccccacgc ggccatgccc
     9001 tcctggtggc agagcgcggg gatgtcgatc ccctcgcgcc gcgccacgtc gagcacggtc
     9061 tcgccctcgc gggcggtgag ctggcggccg tcgacggtga gctgcacggc agccttggtg
     9121 cgcgggctca ctgctgcctc ccttcggcca gcgccggttg cgcgtcgacg acggggatgg
     9181 cgtcgaactt gcacacctga cggcaggtgt cgcacttgat gcagagcttc tggtcgatca
     9241 catgagcctc tttgcgctca ccgctgatcg cctgcgtggg gcaggccccg aagcagacgt
     9301 ggcagccggt gcaggcgtcc gggtcgatgg tgtacttgat gagcggccgg cacaccctgg
     9361 cggggcagcg gtgctccacg atgtgggctt cgatctcctc gcggaagtag cggagggtgc
     9421 tgagcaccgg gttgggcgcg ctgccgccga gctggcacag cgagccctcg atgatgtcct
     9481 cgctcagcgt ctcgaggcgc tcgaggtcgt cgagggtgcc gaggccgtcg cagatgcgct
     9541 cgatgatctc gagcagccgc tggctgccga cgcggcacgg cacgcacttg ccgcagctct
     9601 cctcggcggt gaacttgagg aagaacttga cgaagtcgac catgcacgtg ctgtcgtcga
     9661 ccacgaccat gccgccggag cccatcatcg agccggcggc gaccaggctc tcgaagtcga
     9721 tcgggtcgtc gagcagagag gcgggcaggc agccgccgga cggcccgccc agctgcacgg
     9781 ccttgaactc gcggccgggc agcatgccgc cgccgatgtc gaagatcacg tggcgcagcg
     9841 tcgcgcccat cggcacctcg atgaggccgc cgttcacgac cttgccggtg aggctgaaga
     9901 tcttggtgcc cttgctggtc tccgtcccct tggcggcgaa cgcctgcgcg ccgtgattga
     9961 cgatccacgg cacgttggcg tagctctcga cgttgttgag gttggtgggc tggccccaga
    10021 ggccctgggc gaccgtgcgg atgtgcttgc cgcgcggcat gccccggtgc ccctcgatgg
    10081 aggccgtgag cgccgtcgac tcgccgcaca cgaaggcgcc ggcgccctgg ttgatgcgga
    10141 tgtcgaagct gaagcccgag ccgaggatgt tctcgccgag caggccgcgc tcgcgcgcct
    10201 gcgccagggc cgcgcgcagc cgttcgacgg cgaaggggta ctcgtggcgc acgtagacgt
    10261 agccctcgac cgggccgctg cccgcgccga tggcgtaggc ggcgatcgtc atgccctcta
    10321 tcacgctgtg cgggttgccc tcgagcaccg agcggtccat gaacgcgccc ggatcgccct
    10381 cgtcggcgtt gcagatgacg tagtgcacgt cgccggggtt gccgcggcag aagcgccact
    10441 tgcgccccgt cgggaagccg gcgccgccgc gtccgcggag gccgctggcc tcgacctcgt
    10501 cgatgacccc catggggtcg ccctcctcga gcaccttggc cagcgcggtg tagccgccgt
    10561 ggttcaggta gtcgtcgacg gagtacgggt cgatcttgcc gttgagcgcg aggacgatgc
    10621 ggtcctgcag cgcgtagaag gggatgtcct tctcgtgcgc caggggctcg ccgctcgcgg
    10681 ggtccttgta gaggagccgc tcgacgatgc cgtcgccgac cacgctggtc tcgatgatct
    10741 cggcgatgtc cttgggcttg agccgcgggt agaagaggcc ctgcgggcgg acgacgacga
    10801 tcgggccctc ctcgcagaag ccgtggcagc cggtctcgac gacggtgacc gtgtcggtca
    10861 ggccacgctc ggcgagcgcc ttgtcgaggg ccttgcgcag ggccaggcgg cccgaggcgt
    10921 ggcaggccgt gccggcgcag acgcggatct cggccttctt gtcgtcggcg aggtaggcgc
    10981 tcttgtcggc tgccgccttg agcgccttgc cgatcctggt ggagggcttc gcgagcggcg
    11041 tcatgcgggc acctccgcct tgcgcagctt gcggaccagc ttgcgcgcct cgttgggtcc
    11101 gacgcgcccg atggtctcgt cctctccgag gcgcacgacc ggcgccaggg cgcaggcacc
    11161 gacgcagttg accgtctgca gcgtgaagag gccgtccggc gtggtcgcgc cgaccggcac
    11221 ctcgagctcc tggctgaagg cctcgaccac gagcggcgcg ccggcgacgt ggcaggcggt
    11281 gcccatgcac acgtcgatga tgtacttgcc gacgggcgcg aggcggaact gaccgtagaa
    11341 ggtggcgacg ccgtagacgc gcgagaaggg caggcgcatc tcctcggcga tgttctcgag
    11401 cgcgacgcgg ggcaggaagc cgtacgcctc ctgcgtggag tgcagcgcct gcacgaggcc
    11461 ggacccgccg cggatgccgg cgatcacggc gtcgaacagc ttcatgtcga tctccggcat
    11521 gaagcccgcc ggctgctcgc ccatgacgca ggccgcgttg gcgccgcagc tctcgcccgg
    11581 gagcgtggcc agggcggcgg cgcgctcggc ctcgacgtcg aaggggagct cctcgaactc
    11641 ggggcagtaa tctatctcga tgcagtccac ggggcaggtg cgctcgcaca gggtgcaggc
    11701 gtagcacttg taggcgccgc ccttgtcctt caccaggtcg aacagcccga cgccgcgcgg
    11761 cttgtggacg agctcgcggc gctcgaagtc gatgcccttg agtacctcgt agagcgccga
    11821 tcccttgggc tcggtcgcgg tggtgccgtt ccttctcggc atgccggtca cctcagtcct
    11881 tgcgcagatt gaggtagcgg cgctccgtgg aggcccgctt cgtgctctgc gcagcgttct
    11941 cgagctcggc gaacgcctgc tcgatgttgt ccgggaagat gtcgcgcccg ccgaggccgt
    12001 agatgaacgg cagcacctgg gggcgggggt cgcagtcgta gagggcgctc ctgacttcca
    12061 ggaagacggg gccgccctgc gcgccgaacg agtcggcgcg gtccatgacg cccacgacct
    12121 tgacgtgctc gagcgccctg gcgtactcct cgtgcgggaa cggccggaac gcgcgcacgc
    12181 gcaccatgcc ggccttgacg cccctggcgc ggagcttgtc gaccgtcatc cgcgtggtgc
    12241 cggcggtgga gccgaccacg acgatggcga tctcggcgtc gtcgagccgg tacgggtcga
    12301 gcaggccgta cttgcggccg ctgagctcgg cgtactcgtc ggcgacgtcc tggatcacct
    12361 tgaacgagcg gtccatggcg cggttctgcg ccactttgtg ctcgaagtac cagccgtgga
    12421 ggccgtcgaa cggacccacc gtcaccgggt tcttggcgtc gagcatgggg ttggtcggcc
    12481 ggtacgggcc gatgaaggtc tgcacctgct ccttggtgag catctgcagc ggcccgatgg
    12541 ccccgctgat cacgtagccg tcgtacatgt gcatcaccgg caggcgcacg tccaggtgct
    12601 cggcgatgcg gatggcctgg atgcagttgt cgtaggcctc ctggtggttc tcgccgtaca
    12661 gctggatcca gccggtgtcg cggccgccca tggtgtcgct gtggtcgcag tggatgttga
    12721 tggggccgga cagcgcccgg ttcaccgtgg tcatgacgac ggggagccgg tagctcgcgg
    12781 cgacgaagag gacctcccac atgagggcga agccctggga ggaggtcgcc gtcatcgtgc
    12841 gcgcgccgcc ggcggcggag ccgatcacgg cgctcatggc gctgtgctcg ctctccacgg
    12901 tgacgaactc ggtgccgacc ttgccgtcgg ccaccatctg cgcgtagagc tggacgacct
    12961 cggtctgcgg ggtgatgggg taggcgcaga cgacgtccgg gtcgatctgg cccatcgcct
    13021 cggcgacggc ctcgttcccg gtgaccgcca ggatctcgga ttgggtgctg gccatgttca
    13081 gtccttcttg tcgatcagca tgacgatggc cttggtcggg cactccctgg cgcagatgcc
    13141 gcagcccttg cagtgctcga ggtcgatgcc gacggccttg ttgtcctcga cgaggatgga
    13201 cgcgtcgggg cagtagaagt agcagagcat gcagccgtcg cacttctcga cgtcgatctc
    13261 gggcaccagc gagcgccagc cgcccgtctc gttgtagacg gagttgccgg cctcgacgat
    13321 ggtcgcgccg agctcgtggc gctcggggcc ccactggtcg acgtcgctga tgtcccaggt
    13381 gggcttcttc ttggccggcg ccttgcgcgc cgcgggcttc ttgagcggcg ccggcttctt
    13441 ggcgcctgcc ttcttgtcca cggtcattcg ccctggacct cctcgtagct gcgcgtgatg
    13501 gcggcgatgt tgccctcgac gaccttgggc gggaacttct tgccgaagtc ctcgcgcagg
    13561 aacgtgacga cgtcatcgat gtcgaacagc ccggtgatgc gcgtgagcgc gccgaccatg
    13621 ggcgtgttcg ggatgggacg cttgatcgtc tcgacggcga tgcgcgtggc ggcgatggtg
    13681 aagagccggt tgcccaccag gcccgagtcc ttgcggagct gggccggcga gcgctcgctg
    13741 ttgacgagca ccacggcgtc gtccggcgcg cccttggtga cgtcgacgat cttcaggagg
    13801 gacgagtcga gcacgacgac gatctgcggg tgctcgatcc ccgagtacac ctggatcggc
    13861 gcgtcgctga cgcgcgtgaa cgcgacgatc ggcgcgcccg agcgctccgg cccgtactcc
    13921 gggaacgcct ggaagtactt gccttctccg agcgcgagct cggcgaccat cttggccgcg
    13981 gtgaccgcgc cctgaccgcc ccggccgtgc cagcggatct cggtgagcga ggggggcgcc
    14041 cccacgttcg tcttgccggt cttcgtcgcc atgttcactc ctctccgtgc cgcacgtcgc
    14101 ggcgcccggc gagggcgcgg cccagtgtca gctcgtcggc gtattcaagg tcgccgccca
    14161 cgggcaggcc gctggcgagg cgcgtgacgc gcacgcgctc cggcagcagg tcggcgacgt
    14221 acatcgccgt cgcctcgccc gtcatggtcg ggttggtcgc cacgatcacc tcggcgacgt
    14281 cctcgtccac gcgcgcgagg agctccgcca tgtgcaggtg ctcggggtcg acgccgtcga
    14341 tggggctgag cgccccgccg aggacgtggt agtagccgtg gtactcgcgc gtgcgctcga
    14401 tggtgaccac gtcgaacggc tgctccacga cgcagagcag cctcgggtcg cgcctcgggt
    14461 ccgcgcagat cgtgcacagc tcgccctcgg ccaggttgaa gcagcgcgag cagaacccga
    14521 tcttctcctt gacctcggcg atggccgccg ccagcggcag cacttcttcc gggcgctgcc
    14581 ggaggatgtg gaaggtgagg cgctgggcgg tgcggggacc gatgccgggg agcttggaga
    14641 gctcgctgat cagacgctcg accggtggtg catacattct cgcgaagtcc ccctggacgt
    14701 gactcgccat acacaatggg tgcaagattc gggcgccgtt ccggcgcccg cagaccagct
    14761 tgacagtcta ctcgtttgcg ccggtcacgg caacgcgcgg ggcctgcgtc ccgcctcgcc
    14821 gggccgccgg gtcgccgacg agagccccct tcaccccggc tctgggagaa ggggatcgcg
    14881 cgacgcgccc ggcaggacgc ctttcagact gcttcagtcc gatattcaga ttctaaacgc
    14941 gtgcccccga gcctgcaagt ccggcgccgc cctgccggcg agtccggcgc ccgctgtgcc
    15001 cgctgccgtc tgccggcgcc gagtccggcg cccgctgtcc cgccgcgccg agtccggccg
    15061 gggggcggga tatccacgcg gcgtttgacg ccgcccacgg catcacccat ggcgccaccc
    15121 gccgcgcgca taggttcctc gccgtggctg gcgactccgg cgccgcgccg ccgccggcag
    15181 ccccggggcg ggcgacgtcg tgactcagaa gagcccgggg atgttgggca ggtcgatgcc
    15241 gccggtggcg ccgccgagct tggtgctggc gagctcctgg gcggcgcgga gcgcctcgtt
    15301 ggtcgcggcg accacgaggt cctgcagcat ctcgacgtcc tcgggatcca ccgcgtcggg
    15361 gttgatgcgc acctctttga cctcgaggga cccggtgacg accacggtga ccacgccgcc
    15421 gccggcgctg gcctccacgg tctcgacggc gagctcttcc tgagcttgcg ccatcttgcg
    15481 ctgcatctcc tggacctgct tcatcatctt gttgtactgg ggattggcca cggcgttctc
    15541 cttagggctc ttcgggcagc agctcggcgt cgagcttggc ctgcgcggca cggatctggt
    15601 cggtgaagtc gggaagcgcc gccggggcct gcggggcggc ggccggagca gcctccggcg
    15661 tggccgcggg gccgccaccg gaggcgagct cgaaacgcgc cgtgacgcgg cgtcccagga
    15721 cgccctccac ggcggtggcg agcacctgcg cgttgtcgcc ctgctcggcg ttgcgcaggg
    15781 cgaactccga gggcagcgcc acggtcaggg cgtcgccctc cagggcgtgc ggccgcccgt
    15841 cgcgcagaaa gccgtagagc ggcaccctgg cggactggac gcgctgcagg atcagctccc
    15901 acgcgcgctt gacgcgctcc agcgtgagct cctgcgcggc gtcggccgcg gtggccggtc
    15961 gtggtggggc cgcggcggga tcgggctgag cctcggccgg agcgggtccg ggcggcgcca
    16021 cgtcggcagg ctgcggcttc gcggggacgg ccggtgagcc gggcgtcgtc gcgggcggcg
    16081 tcgcgggcgc ggtggcggcg gcgtcgctcg gcggacgtgc gggcgcggcg gcgtcggtcg
    16141 ccggacgtgc gggcgcgggc gcgggcgcgg atgaggcggg cccggccgcc gccggcggcg
    16201 cgggcgccgc ggggccgccg ccggtcgccg cggcctcgag acgccgcagg cgctcctcga
    16261 gggcggcctc cgagtggtcc aggtccggcc gcgtgacctt gaccagcgcg agctcgagct
    16321 gcagccgcgg gtcgagaccg tcgcggatct cgcgctgcgc ctcgccgagc tgctcgatga
    16381 agaacacgac ctcgcgcggc gagacctgcc cggcgcgctg cgcctgctcg cggaacagct
    16441 cgccgtcgag ctccacggtc tggccgaggg tgcgcagcat cgcctcgtcg ctcgccgcct
    16501 cctcgaggtg ctggaggagg aacagctggc gcaggtggcg cagcaggtcg cggatgaact
    16561 gcgagtagtc ggtgccctcg ttggcgaggc gctgcacgaa ctggagcgcc tcggccgtgc
    16621 gccgctcggt gacgatgtcg gtgatctcga agaggaggtc gctgctggtg acgccgagcg
    16681 cctcgagcac gtcggcggag gtgatcgtgc cctcggcgta ggtcaccagc ttgtcgagcg
    16741 tgccgatggc gtcccggaac cccccctggg agtggcgcgc gatgatgtgc agcgcctcct
    16801 cctggatgtc gatgggcggg ccgccgcgct cgtcgtccgg gcgcgcgttc tcgcgctcga
    16861 cgatgcccgc cagcaggcgc acgatgccgg cgacctgggg gcggtggaag tcgaaccgct
    16921 ggcagcgcga gacgatggtc tcggggatct tgtgcgcctc cgtggtcgcc agcacgaaca
    16981 cgacgttgtc gggcggctcc tccagcgtct tgaggagggc gttgaaggcc tcacgcgtga
    17041 gcatgtggac ctcgtcgatg atgtagacct tgaatcggcc gtcgaccggc gcgaaggcga
    17101 tcttgtcgcg caggtcgcgc acgtcgtcga tgctgcggtt ggaggccgcg tccatctcga
    17161 ccacgtcgag cgagacgccg tcggcgatgg cgacgcagtg gcggcagacg ccgcaggggg
    17221 tgatcgtcgg actgtcgttg gcgccgtctg cgcccacgca gttgagcgtc ttggcgagga
    17281 tccgcgcggt actggtcttc ccggtgccgc gcggcccggc gaagatgtag gcgtgcgcca
    17341 cctggccggt ctccaccgcg ttgcgcagcg tgcgcgccac gtggtcctgg ccgacgagct
    17401 cggagaacgt ctgggggcgg tactccctgt acagcgagcg cttcatggga tggctagtgt
    17461 accccgggag ccggtcagga gatgacggcg aaatcgatga atccgcgctc cgggtcggcg
    17521 tcgacgagac gcacgcgcac cgcgtcaccc acgtccagct cgccggcgcc ctccaccacg
    17581 cggccttcca ccggtggatc gagcgcccgc acccaggtgc ccttggggct cgcgccggtg
    17641 acgaagccgt cgaactcgtc gccgacccgc tcgacgagga agcaggcggc ggccgacttg
    17701 cgcacgcgcc gctcgacctt ctgcgcctgg tcctcctggt gcgtgcagtg cgcggcgagc
    17761 tccgccagcc gctcgccgct gtacgggatc ggcgacccgt ccagcgccgc cttgaccagg
    17821 cgctgggtga tgaggtccgg gtagcggcgg ttcggcgccg tggagtgcgc gtagtgggtc
    17881 acggccagcc cgaagtggcc gtcgatgggc tctcccggct gcgtcgcctc gtactcgccg
    17941 ctgccgagca gcttgacgat cgccagggag aggtcgggga agcgctcggg atcgctctcg
    18001 cgctcctcct ccaggaacgc gttgagcgac ggcgcgtcgg gctcctcggg gaggaggaag
    18061 ccgtgctggt cggcgaggtc gacgatgcgc tgccagcgct ccggctcgcg caccacgcgg
    18121 cgcaccaccg ggaaccgctt gcccgcgagg tagcgagcga cgacgccgtt ggcggcgatc
    18181 atgaagtcct cgatgagctc cttggcgcgg tcgcggggct cgacgacgag gccgctgacg
    18241 gcgccgccgt cgaagcgcgc cttcacctcg agggtctcga ggtcgagcgc gccctgctcg
    18301 cggcggcgcg cccgcaggcg ctgcgccacc gtgtgctgca ggcgcaggtt ctcggcgagg
    18361 ccctccacgg ccgccaatgc cgggggcatc ccgccctcgc cctgcagcca ggccccgacc
    18421 gacgggtagg cgagcttggc gtggttgtgc acgagggcgc ggcgcacgtc ttcggccacc
    18481 accgcgccgc cggcgtcgac cgtcatcccg acgacgacgg cgagccggtc ctcggcgggg
    18541 ttcagcgagg tgaggtcggt gctcagccgg tccggcagca tggggaagat gcgccccggc
    18601 gtgtagaccg aggtcgtgtt gacgccggcg tgcgcgtcga cggcggagcc cggcaggacg
    18661 gtcgcgctca cgtcggcgat cgcgaccagg accctgacgc cgccgtccac ctcgccctcg
    18721 gcgacggtga gctggtcgag gtcgagcgag tcgtcgttgt cgatggagca ccagagcagt
    18781 gcgcgcaggt cggcggcgcc ctcgccgtcc gctgaggcct ccgggagcgc cccggcctcg
    18841 gcgatcgccc gcgccgggaa ctcaggctcc aggccgtact cgatcatcgc ctcgcgggcg
    18901 acgcgcgcca gcagttcacg gtgctggtct gcgggctggt tcacgggcgc tcctccttct
    18961 ccgggccgca ggtcgcgcac tcgtcctccg gcgtgaccga tctggcgagc gccggcgcgg
    19021 gctcggcctc gtcctgcggc cgcagccgcg cgcgcagcac ggccatcgcc tccgccggcg
    19081 tgtcgacgaa gtgcagcagc ctgaggtcgt cggcgctgat ggtcccgtgg cgcaccaggg
    19141 cgtcgaagtc cacgatctcg ttccagtagc gggtcccgta gagcagggtg gcgaccggcg
    19201 ggtcgatctt gccggtctgc gacagggtga ggaactcgaa cagctcgtcc agggtgccgt
    19261 atccccccgg gaacacgatg atcgcccgcg ccacgtaggc gaaccacagc ttgcgcatga
    19321 agaagtagcg gaactcgaac gagagcccct ccgtgatgta cggattgggg cgctgctcgt
    19381 gcggcaggcc gatgttgagg ccgatggtgc ggccgccggc ctcgtgcgcg ccgcggttgg
    19441 ccgcctccat gatcccggga cccccgcccg tgcacacgag gtagcggtgc gcggggctcg
    19501 tgagcccgtc cgaccactcg gtgatcaggc gggcgagatc gcgggcctcc gtgtagtagc
    19561 ggcccagagg cccgtcctcc gtgatccgcg ccgagccgaa gaagacgatg gtgtcgtgga
    19621 tgcggcggcg acggaacacg ccgagcggcc gcaggtactc ggccaggatg cggagggggc
    19681 gaccctcgtc gctctcgacg aaggcggcgt cctggtaggc gagcgggtcc cggtccgcgg
    19741 gggacgcggg gtcgggcgga agcttcatga gactgtgtcc cccggtcgtc agaggtgaaa
    19801 aaagcggccg cgcacccgcc gtcgaagcgc gtcctccgaa cgtcctcgcc gtgcgcaagc
    19861 tcgggccagg cgaccctgcg gcacatggaa tggcctgctt accgctgctt cctcccggac
    19921 ctgacggggt tcgcagggtt ccattgcgta ggacccgacc ttcaacgccg cgacacggct
    19981 gactgcctcg cgggacggcc cctcgggcgg ggattcagcc ccgctgtagc ggattgcggg
    20041 tacagggcac cgctggctcc ccgcctagcg cggccacctg taggctaccc gtggtcatcc
    20101 ggggaagcaa acgtcggcgc ggcgccggcg agctcggcgg cacggtcgac ggcggccacc
    20161 acggcgtccg gcgccgtgaa ctggggcatg tgaccggcgc ccgccacgcg caccagctcc
    20221 gaacccggga cgagggcgtg cagccggtcc gacaccgcag gggggacgat ccggtcctgc
    20281 gcgcccacca ggatcacgag cggcgcccgg aggcccgggt agagcggccg cagcgccgcg
    20341 aggccgctct ccacggcctt gcggtcctcg ccgtcgctga tcagtgccga cgcctggagc
    20401 gccagctgcg gcgcgatgcg cacgtactcg tccggcgggg tcccgggaga gaacgcgcgc
    20461 tcgacggcgc tcctgacgag cgggcgcccg agccgggagc ggccgaggag gcccacgccg
    20521 cggagcgtcg ccgggtggcg caggagcgtg gtgacccacg gcgggccgcc gcccagaggg
    20581 agcacgtagc cgcagagcgt gaccacggcc gccacctcgt ccggcgccgc cagcgcccag
    20641 gccagcgcca cggcggcgcc gagagagtgg ccgatcagga tgggacgccg gagcccgagc
    20701 tccgaggcgg cggcgcgcag caccgccgcc tgcgcctggg gcgtgttccc gccgttcgcc
    20761 ggccgggcgc tgaagcccga ccccggccgg tccatggcga gcgcggtgta gcgctccgcg
    20821 aggcgctcgc ccaccgagag cgtgaagtcg tagacggacc ccttggcgcc gtgcacgtag
    20881 accaccggcg gccccgctcc cccacgcacg tagtgcacgc ggacgccgcc ggcgacgacg
    20941 aagtcccccg gcgccgcggg cccccgccac ccgccagctt tcgcgaagct ccccatgagg
    21001 ccagtctcca cctgcggggc gtgttgcaaa ccagcgaccc gcggctacag tgggctcgtg
    21061 ggcactcgtc ggtacctgtt cgccgggctc atcgtgggcc tggtcctcgc agtcgtgatg
    21121 gtggccatca cgcccttcgg cgcgccacga gcggcgccct cttccgctcc ggccggccgg
    21181 ttctcctcgg cccgcgccgt ggaccacctg cgcatcgtcg ccgggggctc gcggccgatc
    21241 ggatctgcgg gcgccgccgc ggccgccggc tacatcaggc ggcacctcga gagccttggc
    21301 ctggaccctc acacgcagac ggcgaaggtc gtctcggcga tcgacccgcg cgtggccggc
    21361 atggtccaca acgtggtcgg ccgtctcccg ggcaccgacc cctcgcgcgc cgtgctgctc
    21421 atgacccact acgactccgt gcccacctcg gtcggggcgg cggacgacgg cagcggcgtc
    21481 gccgccctcc tggagacggc gcgcgccctg cgctcgggcc cgccaccgcg caacgacgtc
    21541 atcttcctgt tcaccgacgg tgaggagcag ggactgctgg gagcgcgggc gttcctgcgc
    21601 gacgacccct gggcctacgc cgtcggcgtc gccttcgact tcgacagccc cggctcatcg
    21661 tcgcctgccc tcatgtacga gacgagtccc ggcaacggcc tgctggtgcg cgagtacctg
    21721 gcggcgacgg acccgttctc gtcgtcgctc atgtacgagg tctcgcgccg tcagcgcatc
    21781 gtcggcgact tccagctctt cctcgcccga gggatcccgg ggatgagctt cggcatgctg
    21841 gacgggccgg gctactacca cacggcgtac gactccctcg cctccttcga cgaggcggcg
    21901 ctccagcacg agggggagac cgcgctgtcc ctggcgcgcc ggttcggcgc cctggacctg
    21961 tggcacgtcc gccggccgga cgtcgtctac ttcaacgtca tcggggacgt cgccgtcgtc
    22021 tacgcgcggt cgctggtggc gccgttcgcc gcgctcgcgg tgctgctgtt cgcggcggcg
    22081 gtggggaccg ccgccgcccg ccgtctgctc tccctgcgag gcctcgccgc ggccgcgctc
    22141 ggcaccggcg cgacgctggt ggcttcgctc ctggtcacgg ccgtctcctg gtggatgtac
    22201 gagtcggcgt accaggagcg catctggacg cggaccggcg tcgtgatcag cgactggtac
    22261 cgggtcggcc tcgtgctcgt ggtggcggcc gtggtgctcg ggatctacgg cttcctgctg
    22321 cgccgtctgc acgcctggga cctcgccgtc gcggccttgg cctggtggtg cgcgggggcc
    22381 gtcggcgtga gcctgctctt tcccggggcg agcttcctgc tcacctggcc cctggtgggc
    22441 ggctcgctgg gcctcatcgg ggccgccctc atcgagcggc cgtcaggcga ccggccggcg
    22501 gcggccctca tcgccctggc cggcgccgtt cccggcctgg tgctcctcag ctcggcggtg
    22561 tacctcctgc tcatgagcgc cggcctcaag cagggcgtca cgctggtcgc ggtgtggctg
    22621 cttgcggggc tcctcatgct gccgctcgcg gtggtcgagc gcgcgttccg cttctggccg
    22681 ccgatcgtgc tggccgtcac cggcgggatc gtgctcttcg ccgtcggctc gaccgtcgcc
    22741 tttgacgccg agcacccgaa gttcaccagc gtctcctacc gcgtccaggc ggacggcacg
    22801 ccggtctggc aggcgatcgg caggctcgac gactacaccc gcgcctacgt gcatccccgg
    22861 ccgggcgccc ccccggtgca gtcctacttc cccgacctcg gcgtgcgcga caccgtggtc
    22921 ggtgaggcgc cgtactacgg gctgactgcg ccgcgcctgc gtatgctctc ggaccacgtc
    22981 gagggcgacc gccggaccgt gcgcgtgcgg ctgcgcccca cgcgcgacgc ggcggtcgtc
    23041 agtctgctcg tgcacacggt ggtaggcaat ctcaccgcga gcgtcgacgg gcacgagctc
    23101 ggcggtcgcg acaccacgct gctcgacgtg accacggtgc gctggtggtt cgacttctac
    23161 gcgcctccgc ccgagggggt cgtcgtcacc ctggacttcg tcgccggccc ccgcgtgctg
    23221 ctccgcgccg tcgacttcgc gcacggcctg ccggccgggg ccgcgggcac gtatcccgcc
    23281 cgcccggccg gcatgctgcc cggcgggatc ggcgacgggt cgttcaccga gacggaactg
    23341 cgcctgccgc gcgtcgcgcc gtaagaccgg tcagagccgg agcaccagct cccggacctc
    23401 cggctcgtcg cccggccgca cgtcgaaccc gaggtcctcg gcgaagcgcg tcatgcccgg
    23461 gttggccgcc agcacgtggc cgcggatctc gcgcacgtgc cggtcgcggg ccgcctggat
    23521 gagcgcgtcc atgagcttgc tcccgatgcc cttgcgctgg taggcgtcgc ggaccacgat
    23581 cgcgaactcc ccctcctcca cgttggcggt ctcgatcgtg agccgcgcga cgccgagcat
    23641 catcttcttc ttcttgccct tgggctcgcg gatggcggcc agggcgatct cgcggtcgta
    23701 gtcgatgtgg cagtagcgca ccagcatcga cttggtgacc tccttgatcg gcccgaagaa
    23761 gcggtactcg gccgtggcgg gggagaggga ctcgatcatg tcggcccaca gcggctcgtc
    23821 ctcgggcttg atcgcccgga tcaccacgtc ctcgccgtcc gtggggacct cccagcggta
    23881 cttgctgggg tacatggaga tcatgaggtg ggcgcccggc agggcgatct tgcgcacgtc
    23941 cttgggctcg atgacgaggc gcgcgtcgag cgcgcacacg ccgtcggtgc gcacctgcag
    24001 cgggttgatg tccatctcga ggatctccgg gaagtcgacc agcaggtagc tcatcttcac
    24061 cagcgcctgc tcgagggaga ccatgtcgac cggcgccttg ccgcggtagc cgcggagcag
    24121 ctggctgacc ttggtgctct ggatcatcga ctgcgccagc gcctggttga ccggcgggaa
    24181 gtcgacggcg acgtcgcggt acagctcgac gccggtgccg cccatgccga acatcatcgc
    24241 gggaccgaag gtggggtcct tcttcgagcc gaggatgacc tcgtagccgc cccggctcat
    24301 ctcctggacg gcgacgccga tgatggtcgc atccggctgg gcctcacgga cacggtccgt
    24361 gatcttcacg aactgattgg ccgcctcggg gccggagcgg acgttgaggg cgatgccgcc
    24421 gacgtcgctc ttgtgcgtga tgtcgtggct gaggatcttg atggccacgg ggaagccgat
    24481 ctcctccgcg gccttggcgc actcctcggc cgtctttgcc accagcgtcc tgacggcgtg
    24541 gatctggtac gcctcgagca cggccttggc ctcaggctcg ctcaggatgc tgcgcccgtc
    24601 gcgggccacc tccaggaaga tcctcttgac cgcgtcgcgg ttcggctcga agtcgggcag
    24661 gatgtcggcc ggtgtctcgt agaggttccc gaggttgcgc gtgtactggt acatgtacat
    24721 gtaggcgccg accgcgtcct cgggcgtgcc gaaggtcggc acgtgcgcgt cgcgcaggat
    24781 gcgcagcgcc tccgccacct tgatctcccc catgaacgag gtgagcagcg gtttgagctg
    24841 gtggctgtgc gcgacgtcga tcagcgccag cgccgtgccc gtcgggtcgc tcatcgcctg
    24901 tggcgtgagg atggcgagca cgccgtcgca gttggggtcg tccatgagcg cctgcgccgc
    24961 ggtggcgtag cgcaccgcgt cggcgtctcc ggcgacgtcg acgggattgc cgcgcgcggc
    25021 gaagccgggc agcgcgtcct gcagcttcac gtcggtctcc ggagcgagct ccgcgagctc
    25081 gccgccgagg tgcagcagcc ggtcgcacgc catgacgccg gggccgccgg cgttggtgac
    25141 gatgcccagg tgcggaccgc gggggctgct cacgcggctg agcgcctcgc tggcgtcgaa
    25201 caggtcctcg atctggtcga cgcggaccac gccggcgcgg cggaagacgg cgctgtagag
    25261 cgtgtcgtcg ccggcgatcg cgccggtgtg gctggcggcc gcgagcgcgc tgcgcgcggt
    25321 gcggccgctc ttcactacga tgatcggctt gctcttggcg aagtgccgcg ccgcgctcat
    25381 gaacttgcgc gcgtcggtga tcgactcgat gtagaggatg atgctgctcg tgtgcgcgtc
    25441 ggcgccgaag tagtcgatga ggtcgccgaa gtcgacgtcg ctcatggagc cgacgctgac
    25501 gaaggcgctg aagccgatgt tgttggccgc cgcccagtcg aggatggcgg tgcccagcgc
    25561 gccgctctgg ctgaagaaag cgacgcggcc ggccggcggg atcacgtggg cgaaggtggc
    25621 gttgaggttc atcgccgggc ggatgtagcc gaggcagttg ggaccgacga tgcgcacgcc
    25681 gtagctctcg gcgatggact tgacctcgtc ctcgagccgc ttgcccgcct tgccgatctc
    25741 cttgaacccc gagctgacga tgacggcgcc ggccacgccg gcctcgccgc actcgcgcat
    25801 ggtctgcggc acgttcttgg ccggcacggc gatgatggcg aggtcgatct tgcgcggcac
    25861 ctggctgatg ctggcgtagg cctggatgcc ctggaccgac tgcgccgaga cgttcaccgg
    25921 gtagacgatc ccctggtact cggcgccgat caggttgcgc aggaggatgt agccgacaga
    25981 ccccttcttg ttgctcgcgc cgatgacggc gatgctcttg gggtcgaaca gcttgtcgag
    26041 attgcgtgtg ctcatctgct ggtcccgtct ttcgtggttg ctctgctccg ccgcgtcgtg
    26101 cgtccacgac gagccgccgg ccggcgaccc gcggggtgag tccgagcgcg actgcggggc
    26161 ctcggcggtg agctgctccg cgcgttgggt gtccggctcg agcttcttgt gaagtttaac
    26221 gcttgcgggc gcgcggttca ccctcttcgg ccgcacgggc ggacgtttcc tgccctccga
    26281 cccgggtaag accgggccgt acggcactcc ggccccgggg aggcggcatg accgagccca
    26341 tctacgcgca gcgctaccga tcgaagaagc gtctgcccgc gggccgggcc gcggcggcgt
    26401 atgcgggtga ggacgcgcag gggcggccgg tggtcgtcac catcgtccgg ccgctcgacc
    26461 ccgacgtctt cctgcgcagc gtgggcgtgg tgaggtcggt gcgccacctc gacctcgccg
    26521 ccgtcgtcga cgccggccgc gagggcccgg actgctacgt cgtcgcggag gatcccggag
    26581 ggagcgacgc agcgacgctg gccacccgcg ggccgctccc ggtgccgggc gcggcgctcg
    26641 cggcggcctc cgccgccgcc gggctcgcc
//
LOCUS       DASUMX010000153        13712 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_624041, whole genome shotgun sequence.
ACCESSION   DASUMX010000153 DASUMX010000000
VERSION     DASUMX010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 13712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13712
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_624041"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="VFZ86_07355"
     CDS             <1..568
                     /locus_tag="VFZ86_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="amine oxidase"
                     /protein_id="HEX5642141.1"
                     /translation="ALPGREDIFRGFTNVGPSLDYLERAFDEGKYGWYSSRPYIDGAI
                     QSTIDPDMAPPGKHVMSCFIQYTPYHLRESDWDTEKENLGDTVQRTLESFFPGFGDLV
                     LQREVVTPLHIERTVGLSEGNIFAGEFFAPQMFFFRPAPGWSQYRTPIAGYYQCGSGT
                     HPGGCVMGAPGKLAAERILGDRAKARRA"
     gene            complement(570..1505)
                     /locus_tag="VFZ86_07360"
     CDS             complement(570..1505)
                     /locus_tag="VFZ86_07360"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017318354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX5642142.1"
                     /translation="MAVNEHPDMIYRPLGRTGERVSAVGVGGFHLAADQVDERLAVRI
                     VRTAVDRGITFMDNCWDYNDGASELRMGEALRDGYRDKVFLMTKIDGRSGKEAARQLD
                     QSLRRLQVDHIDLVQHHEVIRYEDPHRIFADEGANAALVEARAAGKLRYIGFTGHKDP
                     HIHLHMLDVAADHGFRFDAVQLPLNVMDAHYRSFERQVLPRLVEEGIGVLGMKSMAAG
                     ILLETGLVTPAECLHYALNLPTSVVITGIDSLQVLDQALEAVRTFRPLGEAEVGRLLA
                     RTAPSAARGEFEPFKTTALFDGTAHTPAWLGDEPA"
     gene            1615..2535
                     /locus_tag="VFZ86_07365"
     CDS             1615..2535
                     /locus_tag="VFZ86_07365"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX5642143.1"
                     /translation="MQARPPASAYAAAVGMTVAFGLSFVATKTALKGFEPLLLALLRF
                     TLAGGILWVVWRLRPLREPVTRAELGRLALVGFVSLTVYFSFENTGIARTSASEAAIL
                     IATIPIFVALIGAFLPAERPTGRQWAGILISFAGIVALVLAAGGAGGGSLTGNLLVLA
                     ASLSAGVYAVLARRLLVSRSALFVTTWQNLFGALFMAPLALVEALLLGVRRPTVPAAG
                     GVLFLTVVCSIVAYLLLNYAFRFLPASRVSVFINLTPIVAVASAYVLLGERLTAGQAL
                     AAAVVVVGVWLTNSGRRAGDELELRSPSAG"
     gene            complement(2513..2998)
                     /locus_tag="VFZ86_07370"
     CDS             complement(2513..2998)
                     /locus_tag="VFZ86_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642144.1"
                     /translation="MRNADYKIELFIFPDGTRVEMIVFDAAARARGAAQAAQASAGPA
                     AVAPARPAPAPCRVPPPPQEDPDAHHCPVCRSPLVYPLDWDRSGPASWTMILRCPECE
                     TRREVTLGRASVEELNRELYQGAQAIAREADRLTRRNFEDEVERMVAALECDLILPMD
                     F"
     gene            3179..4036
                     /gene="murI"
                     /locus_tag="VFZ86_07375"
     CDS             3179..4036
                     /gene="murI"
                     /locus_tag="VFZ86_07375"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEX5642145.1"
                     /translation="MTASSSDSLHDPAALPIGMFDSGMGGLTVLHECLVQLPGEHFAY
                     VGDTARFPYGEKRPAELELFSRQITAFLETVPVKLIVVACYSATSAALPALQEQFETP
                     IIGVVMPGARAAVATSRYRRIGVLATEATVASGAYPRAIRSLDSGAEVIQQACPGLVD
                     FIEAGDVASQALADAVRGFTEPLKAQRPDVVIMGCTHYPLIAPMLQRFLGRDVTLINP
                     AAEIAREVEAMLVRQGLERAGDAIGSYRFYTTGDVERFRDTGARFLQMPLTRVRALPL
                     ERLGELVSP"
     gene            4033..4515
                     /locus_tag="VFZ86_07380"
     CDS             4033..4515
                     /locus_tag="VFZ86_07380"
                     /inference="COORDINATES: protein motif:HMM:NF017941.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEX5642146.1"
                     /translation="MTSRFLVRAAVYAALYAALTLAPGLNAFAYGEVQFRVAEALMVF
                     ACFDPAAVLGLTLGTAIGNLGSPMMPVDVVVGAALTLVAAAVMHAIGPRVIALAAPVV
                     VNALGVAAMLAIILDLPYWASAVWVGVGEAAVLFTLGLALLLVVRGRREVFGFTRPAA
                     "
     gene            4555..5280
                     /gene="rph"
                     /locus_tag="VFZ86_07385"
     CDS             4555..5280
                     /gene="rph"
                     /locus_tag="VFZ86_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006215193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEX5642147.1"
                     /translation="MRKGRKPDELRPVRITPAVAKFAAGSARIELGDTHVFCTASLSD
                     GVPGWLRGSGRGWLTAEYSMLPGATPTRTAREAVRGKQSGRTMEIQRLIGRSLRGVMD
                     MGIIGERTIAVDCDVLQADGGTRTASITGAYVAVYQVALKIKDLLGLHEVPLFDSVAA
                     VSAGIVDGEARLDLDYEEDSAAEVDLNAVITGDGRLVEVQATAEGEPYSRAALDGMLD
                     LVTAGAASLAAAQAAACAELEGA"
     gene            5280..5948
                     /locus_tag="VFZ86_07390"
     CDS             5280..5948
                     /locus_tag="VFZ86_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HEX5642148.1"
                     /translation="MRFVLATGNRHKASEFAAILAPCRVDVMPAGTELPPEGVESFAE
                     NALGKAEALARAVEGDPALLRRLGLRPDEEVLFLADDSGLEVEALGWAPGVTSARYAG
                     VDGPGADRANYERLLGEMSGLAGPLARRARFVCAVVAVAPDMTHWAATGHWWGAIAEA
                     PRGEGGFGYDPVFVPEGSDLSVAEWPQDAKDQASHRALAGRALVDVLRREGFLHGDEG
                     QQVC"
     gene            5920..6864
                     /locus_tag="VFZ86_07395"
     CDS             5920..6864
                     /locus_tag="VFZ86_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012532323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX5642149.1"
                     /translation="METKANKSADAAVVVDDRHNHSLKSRAAAVSIASNATLIVFKLV
                     VGLMSGSIAIISEALHSGSDLVAALIAFWSVRQAAQPPDERHHYGHEKVENLSGVIEA
                     LLIIAAAGVIIFEGVMKIIDGPELDHIWLGIGVMAISGVVNLIVSKKVLYPVARRTES
                     AALEADAAHLLTDVYTSFGVAFGLLLVKLTGWPYFDPIAAIAVAVLIIKTGYDLVMQS
                     TRVLLDETLPDDELEEIRRCVREHRGDIISGYHKLRARRAGSRRHVDLHVVVDADLTV
                     TEAHDIAEHIEDDIRACIPNTDVLVHVEPRSPDRFEDA"
     gene            complement(6917..8188)
                     /locus_tag="VFZ86_07400"
     CDS             complement(6917..8188)
                     /locus_tag="VFZ86_07400"
                     /inference="COORDINATES: protein motif:HMM:NF013715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEX5642150.1"
                     /translation="MARTPRRVGGLRLAGRVGRTRLLVIFALVGPGFIAANAGNDAGG
                     ITTWSVIGSRYGYSMIWLLVAITPILAIVQEMNARVGVVTGRGLAGLIRENFSIKITA
                     LAILATVVANFGTTVAEFSGVAAASGLLHVPSYVAVPVVAVGVWLLVTRGSYRKVERV
                     LLVLGFVLLTYVVSGILAGPDWGQALHGATHPYISWDSLWIFTVIAAVGTTLTPWGQF
                     FIQAAVVDKKVPWRQFKYTKYEIYAGSVFMTTIDFFIVLACAATLYEDGIVVRSAEEA
                     AMALVPFLGAAAQYLFAVGLLSVSILAAGVLPLATAYVVCEAFGFESGLDNSFREAPV
                     FNGLITFLMFVPAAVAIIPGLPLVDVILVAQSLNGVLLPIILIFTLLIINDPRVMGAH
                     VNGRLRNVIAWGFTVALIGMSAVLLLSPLFS"
     gene            complement(8199..9449)
                     /locus_tag="VFZ86_07405"
     CDS             complement(8199..9449)
                     /locus_tag="VFZ86_07405"
                     /inference="COORDINATES: protein motif:HMM:NF015413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX5642151.1"
                     /translation="MFYLSKLLGSPVKDVENKSAGTLHDLVVSTGQAYPRVTAVDVRR
                     RGRTLFAPWDAVTSLEESATLLNIPASALAERELEPGELLLSATFLDKQLVDTEGHKV
                     IRVNDIELVRNGPWVQVAAVDVSSNAILRRVGLAKVGDRIAKGRQKPKPHLIDWKDVD
                     IEQTDEAAVRLAVPQTKLELLHPADIAELVHELSPEERAAVFDALEDDLAADTFEELH
                     PSYQASLLLDLPDEKASEILNEMSPDDAADLLADLPDDRRQHFIAMMEKDDAEDMREL
                     LSYPEHSAGGIMTTDYAWARLDDTAERAIERLREQEAEAETVYYIYVVDRSERLRGVF
                     SLRDLLMTPPEKKVRDFMTENPVSVRTAASEEEITNVIAKYNLLALPVVDEEGELHGI
                     ITIDDAIDLVLPLAWKKRLPRIFH"
     regulatory      complement(9536..9705)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            9842..10795
                     /locus_tag="VFZ86_07410"
     CDS             9842..10795
                     /locus_tag="VFZ86_07410"
                     /inference="COORDINATES: protein motif:HMM:TIGR03168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HEX5642152.1"
                     /translation="MIITVTPNAAVDKTLTVPNFRTGFRHRASESLTLPGGKGVNIAR
                     ALKTLGEPMIVTGLVGGRAGQQIVEGLTRENILNDFVHIAGESRTSTAVVDPTTMTQT
                     EVVEYGPVVTAAETAGLLDKIEYLAKGARFVVLAGSLPRKVSEDFYATVLARIRKHRC
                     FVVLDSAGEPLRRGVRGRPNLVIPNLREAEDLVGHEFHDDQDLVDATGIVCEMGARNA
                     IIKTQHGCVARFQVGRKQRVFRASIVHLDAVVSTVGSGDVFLAGFISARFRKLDLPEC
                     LRHALAAGAASTQQYGAGVLDAPTAASLLESAEVKELSLRP"
     gene            10880..11383
                     /gene="fae"
                     /locus_tag="VFZ86_07415"
     CDS             10880..11383
                     /gene="fae"
                     /locus_tag="VFZ86_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019618553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016840 - carbon-nitrogen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-activating enzyme"
                     /protein_id="HEX5642153.1"
                     /translation="MSEVQIGESFIGHGADAAHVNTVLGERGGPVGTAWATALATPRA
                     GHVPFVAVVQPGLAVQPMTLFVNKAPIEPGRHADLTWGAAQAGVAAGVAEAVAAGIVD
                     RDRAGELLVIAAVWVNPAAEDEDAVFANNREATLAALRNGRDGAPLAEDVLAARSDPW
                     NPFFHAR"
     gene            11398..12384
                     /locus_tag="VFZ86_07420"
     CDS             11398..12384
                     /locus_tag="VFZ86_07420"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014920691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5642154.1"
                     /translation="MNARNDKISFVGLGHMGGPMSRRLAEAGLDLTVFDLDPGRAGPA
                     LARGARLAGTVEEAARGAGVFITMLPTPAAVEDVMLGAGRALRALPAGALWIDMSTSS
                     PDVAGRVRVRGAQNGLRALDAPVAGMAAGADAGTLEIFVGGAAADVAQARPILEHLGD
                     PERIFHVGGNGAGYVVKLMLNLLWFDQLVAIAEVLAIGTRAGVDLEVLHRSLVEGPTS
                     SRLLARDLLPLLRDGEYEEGFSMALATKDLRLAVDLARSAGVPAELSAVVEQLFTRAR
                     AVYGDDAGEMTPVRLYEETAGVLLRLPAAGAGRDGPSSGRETPDEGDGERAA"
     gene            12565..>13712
                     /locus_tag="VFZ86_07425"
     CDS             12565..>13712
                     /locus_tag="VFZ86_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
                     /protein_id="HEX5642155.1"
                     /translation="MEVEIGRGKKGRRAYGFDDIAIVPSRRTRDAEDVDITWKLGEHT
                     LELPCLASAMDGVVDPAFAVTMGKLGGLAVLNLEGVQTRYENADEQLDRIASLPKESA
                     TRTMQEIYQEPIKAELVAKRVKEIKDGGVLAAASLTPQRVEDYYEVALEAGLDVLVIQ
                     GTVVSAEHVSTRVEPLDLKKFISGCAVPVIVGGVASYATTLHLMRTGAVGVLVGVGPG
                     HACTTRGVLGIGVPQATAIADAAAARVQHLLETGEYCNVIADGGMRTGGDVSKAIALG
                     ADAVMIGSPLASATEAPGRGYHWGMATFHPELPRGTRVKAGNKGTLQEILLGPAHEND
                     GTRNLFGALRTSMATCGYESLVKFQKAEVMVAPALMTEGKKLQKEQDVG"
BASE COUNT         1940 a         4884 c         4858 g         2030 t
ORIGIN      
        1 ggccctgccc ggccgcgagg acatcttccg cgggttcacc aacgtcgggc cgtccctgga
       61 ctacctcgag cgcgccttcg acgagggcaa gtacggctgg tacagctcgc ggccgtacat
      121 cgacggcgcc atccagagca cgatcgaccc ggacatggcg ccccccggca agcacgtcat
      181 gtcctgcttc atccagtaca cgccgtacca cctgcgcgag agcgactggg acaccgagaa
      241 ggagaacctg ggcgacaccg tgcagcgcac gctcgagtcg ttcttcccgg ggttcggcga
      301 cctggtcctg cagcgcgagg tggtgacgcc gctccacatc gagcggacgg tggggctgtc
      361 cgagggcaac atcttcgccg gcgagttctt cgcgccgcag atgttcttct tccgtccggc
      421 gccgggctgg tcccagtacc gcacgccgat cgccggctac taccagtgcg gctcgggcac
      481 ccacccgggg ggctgcgtga tgggcgcccc cggcaagctt gccgcggagc ggatcctcgg
      541 ggaccgcgcg aaggcgcggc gcgcgtaggt caggccggct cgtcgccgag ccaggccggg
      601 gtgtgcgccg tgccgtcgaa gagcgccgtg gtcttgaacg gctcgaactc acctctcgcg
      661 gcgctcggcg ccgtcctcgc cagcagtctc cccacctccg cctcgccgag cgggcggaac
      721 gtgcggacgg cctccagcgc ctgatccagg acctgcaggc tgtcgatgcc ggtgatgacc
      781 accgacgtgg gcaggttcag ggcgtagtgc aggcactcgg ccggcgtcac gaggcccgtc
      841 tcgagaagga tcccggcggc catgctcttc atgccgagga ccccgatgcc ctcctcgacg
      901 aggcgcggca gcacctggcg ttcgaagctc cggtagtgcg catccatcac gttcagcggc
      961 agctggacgg cgtcgaacct gaagccgtgg tcggcggcca cgtccagcat gtgcaggtgg
     1021 atgtgcgggt ccttgtggcc ggtgaagccg atgtagcgca gcttgccggc agcgcgggcc
     1081 tcgaccagcg ccgcgttggc gccctcgtcg gcgaagatgc ggtgcgggtc ttcgtagcgg
     1141 atcacctcgt ggtgctggac gaggtcgatg tggtccacct ggaggcgacg gagcgactgg
     1201 tcgagttgcc gtgcggcctc cttcccggag cggccgtcga tcttcgtcat gaggaagacc
     1261 ttgtcccggt acccgtcacg cagggcctca cccatgcgca gctcgctcgc gccgtcgttg
     1321 tagtcccagc agttgtccat gaaggtgatg ccgcggtcga ccgccgtgcg cacgatgcgc
     1381 acggcgaggc gctcgtcgac ctggtccgcg gcgaggtgga acccgcccac acctacggca
     1441 gagaccctct caccggtgcg gccgaggggt cggtagatca tgtccggatg ttcgttcacg
     1501 gccattccct cctccgtgcg gccgacagcg acaaagggac tattcccgtg cggcggcatc
     1561 tgggtcggct ccgccaccac gggcggcggc cgggcttggg atacgcttgg gggcatgcag
     1621 gctcgtcctc cggcatccgc ctacgccgcc gccgtcggca tgacggtcgc cttcgggctc
     1681 tcgttcgtcg ccaccaagac ggcgctcaag ggtttcgagc cactgttgct ggcgctgctg
     1741 cgcttcaccc tggcgggcgg catcctctgg gtcgtgtggc gcctgcgccc gctgcgcgag
     1801 ccggtgacgc gggcggagct cggccggctc gcgctcgtcg ggttcgtctc cctcaccgtc
     1861 tacttcagct tcgagaacac cgggatcgcg cgcaccagcg cctcggaggc cgcgatcctc
     1921 atcgcgacga tcccgatctt cgtcgcgctc atcggcgcgt tcctgccggc cgagcggccc
     1981 acgggccgtc agtgggccgg catcctcatc tcattcgcgg ggatcgtggc tctcgtgctc
     2041 gccgccggag gggccggcgg cggctcgctc accggcaacc tgctggtgct ggccgcctcg
     2101 ctctccgcag gcgtctacgc cgtgctggcg cggaggctgc tcgtgagccg ctccgcgctg
     2161 ttcgtcacca cctggcagaa cctgttcggc gccctcttca tggcgccgct ggcgctggtg
     2221 gaggcgctgc tgctgggggt gcgccggccc acggtcccgg cggccggcgg cgtgctcttc
     2281 ctcacggtgg tctgctcgat cgtcgcctac ctgctgctca actacgcgtt ccggttcctg
     2341 ccggcgagcc gggtgagcgt gttcatcaac ctcacgccga tcgtcgccgt ggcgagcgcc
     2401 tacgtgctgc tgggcgaacg gctgacggcg gggcaggcgc tggctgcggc cgtggtcgtg
     2461 gtgggggtgt ggctgacgaa cagcgggagg cgcgcggggg acgagctgga gctcagaagt
     2521 ccatcggcag gatgaggtcg cactccagcg cggccaccat ccgttccact tcgtcctcga
     2581 agttgcgccg cgtcagccga tcggcctcac gggcgatcgc ctgcgcgccc tggtacagct
     2641 cgcggttcag ctcctcgacg gaggcgcgac ccagggtcac ctcgcgccgc gtctcgcact
     2701 cgggacagcg gaggatcatc gtccagctgg ccggcccgga gcggtcccag tcgagcgggt
     2761 agacgagcgg gctgcgacag acgggacagt gatgcgcgtc ggggtcctcc tgcggcggcg
     2821 gcgggacgcg gcagggagcg ggggccggcc tcgccggcgc cacggccgcg gggccggcgg
     2881 acgcctgcgc ggcctgcgca gcgccgcgcg cccgggcggc ggcgtcgaac acgatcatct
     2941 cgacgcgggt accgtcggga aagatgaaca gctcgatctt gtagtcggcg ttgcgcacga
     3001 cgcgcctccg tagcctctcc gttgcccctc gcactccacc tatcggacgc tccccggcgg
     3061 cgcttgagcc ccgcgtgcgc ttcatctcct gggcgcgacc ggtcccccgg aacgggtcgt
     3121 ctggacagtg ccgagcgcgg tgaggaatac taccggggcg cctcacggcg cccctgacat
     3181 gaccgcctcc tcctccgaca gcctgcacga ccccgccgcc ctgcccatcg ggatgttcga
     3241 ctcgggcatg ggcgggctca ccgtgctgca cgagtgcctc gtgcagctgc cgggcgagca
     3301 cttcgcctac gtcggcgaca cggccaggtt cccgtacggc gagaagcgcc cggcggagct
     3361 ggagctgttc tcgcgccaga tcacggcgtt cctcgagacc gtgccggtca agctcatcgt
     3421 cgtcgcctgc tactccgcga cgtcggcggc gctgccggcg ctccaggagc agttcgagac
     3481 gccgatcatc ggcgtggtga tgccgggcgc gcgggcggcc gtggcgacct cgcgctaccg
     3541 caggatcggc gtgctggcca ccgaggccac cgtcgccagc ggggcctacc cgcgagccat
     3601 ccgcagcctc gactccgggg cggaggtcat ccagcaggcc tgccccggcc tggtcgactt
     3661 catcgaggcc ggggacgtcg ccagccaggc gctcgccgac gcggtgcgcg gcttcaccga
     3721 gccgctcaag gcccagcgcc ccgacgtggt gatcatgggc tgcacgcact acccgctgat
     3781 cgcgcccatg ctgcagcgct tcctggggcg cgacgtcacg ctcatcaacc cagcggcgga
     3841 gatcgcccgg gaggtcgagg ccatgctcgt ccgtcagggc ctcgagcgcg cgggcgacgc
     3901 catcggctcc taccgcttct acacgacggg cgatgtggag cgcttccgcg acaccggggc
     3961 gcgcttcctc cagatgccgc tcacgcgcgt gcgcgcgctg ccgctcgagc gcctcggcga
     4021 gctcgtctcg ccgtgaccag caggttcctg gtgcgggcgg ccgtctacgc cgccctgtac
     4081 gccgcgctca ccctcgcgcc cggcctcaac gccttcgcgt atggcgaggt gcagttccgg
     4141 gtcgcggagg ctctcatggt cttcgcctgc ttcgaccctg ctgccgtgct cgggctcacg
     4201 ctgggcacgg ccatcggcaa cctcggcagc ccgatgatgc cggtggacgt ggtggtcggt
     4261 gcggcgctca cgctcgtggc cgccgccgtc atgcacgcca tcgggccgcg cgtgatcgcg
     4321 ctggccgcgc cggtcgtcgt caacgcgctg ggcgtcgcgg cgatgctcgc gatcatcctg
     4381 gacctgccgt actgggcgag cgcggtctgg gtgggtgtcg gcgaggccgc ggtgctgttc
     4441 acgctcggcc tcgcgcttct cctcgtggtg cgcgggcgcc gcgaggtgtt cggtttcaca
     4501 cgaccggccg cctgacgcgg ccgtctgccg cctgcggcgg cgaggaggac gaccttgaga
     4561 aaaggacgca agcccgacga gctccggccg gtgcgcatca cgccggcggt ggccaagttc
     4621 gccgccggct cggcgcgcat cgagctgggc gacacgcacg tcttctgcac cgcctcgctc
     4681 agcgacggcg tgccgggctg gctgcgcggc tccggccgcg gctggctgac cgccgagtac
     4741 agcatgctgc cgggggccac gcccacgcgc accgcgcgcg aggcggtccg gggcaagcag
     4801 agcgggcgca ccatggagat ccagcggctc atcggccgca gcctccgtgg cgtcatggac
     4861 atgggcatca tcggcgagcg caccatcgcc gtggactgcg acgtgctgca ggccgacggc
     4921 ggcacccgga cggcgagcat caccggcgcc tacgtggccg tctatcaggt cgcgctcaag
     4981 atcaaggacc tgctcgggct gcacgaggtg ccgctgttcg actcggtcgc cgcggtgtcg
     5041 gccggcatcg tggacggcga ggcgcgcctc gacctcgact acgaggagga ctcggcggcc
     5101 gaggtggacc tcaacgccgt gatcaccggc gacggccgcc tcgtcgaggt gcaggccacg
     5161 gccgagggcg agccctactc gcgcgcggcg ctcgacggca tgctcgacct cgtgaccgcc
     5221 ggtgcggcca gtctggcggc cgcgcaggcc gccgcctgcg cggagctgga aggggcctga
     5281 tgcgcttcgt gctcgccacc ggcaaccgcc acaaggcctc ggagttcgcc gcgatcctcg
     5341 cgccgtgccg cgtcgacgtc atgccggccg ggaccgagtt gccgccggag ggcgtcgagt
     5401 cgttcgccga gaacgctctc ggcaaggccg aggcgctggc gcgggcggtc gagggcgatc
     5461 ccgcgctgct ccggcggctc ggcctgcggc cggacgagga ggtgctcttc ctcgcggacg
     5521 actccgggct cgaggtcgag gcgctcggct gggcgccggg cgtcaccagc gcccgctacg
     5581 cgggcgtgga cgggcccggc gccgacaggg ccaactacga gcgcctgctg ggcgagatga
     5641 gcggcctcgc cggcccgctc gcccgccgcg cgcgcttcgt ctgcgcggtt gtcgccgtcg
     5701 cgcccgacat gacacactgg gccgcaacgg gacattggtg gggcgccatc gccgaggcgc
     5761 cgcgcggtga gggcgggttc gggtacgacc ccgtcttcgt tccggaaggg tcagacctct
     5821 ccgtggccga gtggccacag gatgccaagg accaggccag ccaccgcgca ctggccggga
     5881 gggccctcgt cgacgtcttg cgacgggagg ggttcctgca tggagacgaa ggccaacaag
     5941 tctgctgacg cggcggtcgt cgtcgacgac cgccacaacc actcgctcaa gtcgcgcgcc
     6001 gcggcggtat ccatcgcgtc gaacgcgacg ctgatcgtct tcaagctcgt ggtcggcctc
     6061 atgtccgggt cgatcgccat catctccgag gcgctgcact cgggctccga cctcgtcgcg
     6121 gcgctcatcg ccttctggtc ggtccgccag gcggcgcagc cgccggacga gcggcaccac
     6181 tacggccacg agaaggtcga gaacctcagc ggcgtgatcg aggcgctgct catcatcgcc
     6241 gccgccggcg tgatcatctt cgagggcgtg atgaagatca tcgacggccc ggagctggac
     6301 cacatctggc tcggcatcgg cgtgatggcg atctccggcg tcgtcaacct catcgtctcg
     6361 aagaaggtgc tgtacccggt cgccaggcgc accgagtccg ccgccctcga ggccgacgcc
     6421 gcgcatctcc tcaccgacgt ctacacgtcg ttcggcgtgg ccttcggcct gctgctcgtc
     6481 aagctcaccg ggtggccgta cttcgacccg atcgccgcca tcgccgtggc cgtgctcatc
     6541 atcaagacgg gctacgacct cgtcatgcag tcgacccgcg tgctgctcga cgagaccctg
     6601 cccgacgacg agctcgagga gatcaggcgc tgcgtgcgcg agcaccgcgg cgacatcatc
     6661 agcggctatc acaagctccg cgcgcggcgc gccggcagcc gcaggcacgt cgacctgcac
     6721 gtcgtcgtcg acgccgacct cacggtcacg gaggcgcacg acatcgccga gcacatcgag
     6781 gacgacatcc gcgcctgcat ccccaacacc gacgtgctcg tccacgtgga gccgcggtcg
     6841 ccggaccggt tcgaggacgc ctgagcggcc gggcccgccg gtggtcgcgg ccggccgctt
     6901 gccgccctct cagccgtcag ctgaacagcg gcgagaggag cagcaccgcg ctcatgccga
     6961 tgagcgccac ggtgaacccc caggcgatca cgttgcgcag gcgcccgttg acgtgcgcgc
     7021 ccatcacccg cgggtcgttg atgatgagca gcgtgaagat gaggatgatc ggcagcagga
     7081 cgccgttcag cgactgggcg acgaggatca cgtcgaccag agggaggccg gggatgatcg
     7141 ccaccgcggc cggtacgaac atgaggaacg tgatgaggcc gttgaacacc ggagcctcgc
     7201 ggaacgagtt gtcgagcccg ctctcgaaac cgaacgcctc acacaccacg taggccgtgg
     7261 ccagcgggag cacgccggcg gcgaggatcg agacgctcag caggccgacg gcgaagagat
     7321 actgcgccgc agcgcccagg aacggcacca gcgccatcgc cgcctcctcg gccgagcgca
     7381 cgacgatccc gtcctcgtac agcgtcgcgg cgcaggcgag gacgatgaag aagtcgatgg
     7441 tcgtcatgaa gaccgacccg gcgtagatct cgtacttggt gtacttgaac tgcctccacg
     7501 gcaccttctt gtccacgacc gcagcctgga tgaagaactg cccccagggc gtcagcgtgg
     7561 tgcccacggc ggcgatcacg gtgaagatcc acaggctgtc ccacgagatg tagggatgcg
     7621 tcgcgccgtg cagcgcctgg ccccagtcgg gtccggcgag gatgcccgag acgacgtagg
     7681 tgagcagcac gaaccccagg acgaggagca cacgctccac tttgcggtac gagccgcggg
     7741 tgaccagcag ccacacgccg acggcgacca cgggcacggc cacgtacgag ggcacgtgga
     7801 gcagcccgct cgccgcggcg acgcccgaga actccgccac ggtggtgccg aagttggcga
     7861 ccaccgtggc gaggatcgcg agggcggtga tcttgatgct gaagttctcg cggatgagcc
     7921 cggcgagccc gcgtccggtg acgacgccga cgcgggcgtt catctcctgc acgatggcca
     7981 ggatcggcgt gatcgccacg agcagccaga tcatgctgta cccgtaccgg gagccgatga
     8041 ccgaccaagt ggtgatgccg ccggcgtcgt tgccggcgtt ggcggcgatg aagcccggcc
     8101 ccaccagcgc gaagatgacg aggagccggg tgcggccgac gcggcctgcg agtcgcaggc
     8161 cgcctacacg gcggggagtc cgcgccatcg aggcctcctc aatggaagat cctcggcagg
     8221 cgcttcttcc aggccagcgg gagcaccagg tcgatggcgt cgtcgatggt gatgatgccg
     8281 tgcagttcgc cctcctcgtc gaccaccggc agggcgagga ggttgtactt ggcgatcacg
     8341 ttggtgatct cttcctcgct cgctgcggtg cgcaccgaga cggggttctc ggtcatgaag
     8401 tcgcgcacct tcttctcggg cggggtcatg agcaggtcgc gcagcgagaa cacgccgcgc
     8461 aggcgctcgg agcggtcgac gacgtagatg tagtagaccg tctcggcctc ggcctcctgc
     8521 tcgcgcaggc gctcgatggc gcgctcggcg gtgtcgtcca gccgcgccca ggcgtagtcg
     8581 gtggtcatga tgccgccggc cgagtgctcc ggatagctga gcagctcgcg catgtcctcg
     8641 gcgtcgtcct tctccatcat cgcgatgaag tgctggcggc ggtcgtccgg caggtccgcc
     8701 aggaggtcgg cggcgtcgtc cggcgacatc tcgttgagga tctcgctggc cttctcgtcg
     8761 ggcaggtcga gcagcaggga ggcctggtag gacgggtgca gctcctcgaa ggtgtcggcg
     8821 gcgaggtcgt cctcgagggc gtcgaacacc gccgcgcgct cctccggcga gagctcgtgc
     8881 accagctcgg cgatgtccgc cgggtgcagc agctcgagct tggtctgcgg cacggcgagg
     8941 cggacggcgg cctcgtcggt ctgctcgatg tcgacgtcct tccagtcgat caggtgaggc
     9001 ttgggcttct ggcgtccttt ggcgatgcga tcgcccacct tggcgaggcc tacacggcgg
     9061 aggatggcgt tgctcgagac gtccaccgcc gcgacctgca cccaggggcc gttgcgcacc
     9121 agctcgatgt cgttgacgcg gatgaccttg tggccctccg tgtccacgag ctgcttgtcg
     9181 aggaacgtcg ccgagaggag cagctctccg ggctcgagct cacgctcggc gagagcggag
     9241 gcggggatat tgagaagggt cgcggactcc tcgaggctgg tgaccgcgtc ccacggcgcg
     9301 aagagcgtcc tgccgcgccg ccggacgtcg accgcggtca cgcgtgggta ggcctgtccg
     9361 gtgctgacca ccaggtcgtg cagcgttccg gcggacttgt tctcgacgtc cttgacggga
     9421 ctgcccagca gcttgctcag gtagaacaca ggcctcacct ccttcgcgtg accagcggtc
     9481 accggacata caaaagcctc cctggccggg aggctccggg cgcgcagagc gaaggggcga
     9541 tcctcactcg tcccagcttt agcacagcac accgtagagg tcagggcctc agccgtttcg
     9601 ggcaaaacct tgtgccggta gtgcctgtcc tacccgttgg cgtctctgga cgtttccggg
     9661 caacggcctc tatgtgtgcc ggagcctcac ctaacgagga atgcacatgg ttgacgctcc
     9721 gagaacgtat cgccgcgccc cggcagtgtc aaggcgcggc gcggtggtat actgggcgcc
     9781 gccgagggcc ggcgccgacc agggcgccgg cttctgtgcg caaggctgac aggaggccag
     9841 cgtgatcatc accgtcacgc ccaacgctgc ggtcgacaag acgctcaccg ttcccaactt
     9901 ccggaccggc ttccgccacc gtgccagcga gagtctcacg ctcccgggcg gcaagggggt
     9961 caacatcgcc cgggccctca agacgctcgg tgagccgatg atcgtcaccg gcctcgtggg
    10021 cggccgcgcc ggccagcaga tcgtcgaggg cctcacgcgc gagaacatcc tcaacgactt
    10081 cgtccacatc gccggcgagt cgcgtacctc cacggccgtg gtcgatccca ccaccatgac
    10141 gcagaccgag gtggtcgagt acggccccgt cgtcaccgcg gcggagaccg ccggcctgct
    10201 cgacaagatc gagtacctcg ccaagggtgc ccgcttcgtc gtgctcgccg gctcgctccc
    10261 gcgcaaggtg agcgaggact tctacgcgac ggtgctggcg cggatccgca agcaccgctg
    10321 cttcgtcgtc ctcgacagcg ccggcgagcc tctgcgccgc ggggtccgtg gccgccccaa
    10381 cctggtgatc cccaacctgc gcgaggcgga ggatctcgtc ggccacgagt tccacgacga
    10441 ccaggacctc gtcgacgcca ccggcatcgt ctgcgagatg ggcgcccgca acgcgatcat
    10501 caagacgcag cacggctgcg tcgcccgctt ccaggtcggg cgcaagcagc gcgtcttccg
    10561 ggcctccatc gtccatctcg acgccgtggt cagtaccgtc gggtccggag acgtgttcct
    10621 cgcgggcttc atcagcgccc ggttccgcaa gctcgatctg cccgagtgcc tgcggcacgc
    10681 gctggctgcc ggcgccgcga gcacgcagca gtacggcgcc ggcgtcctcg acgcgcccac
    10741 cgccgcctcc ctcctggagt ccgccgaggt caaggagctc tccctccgcc cctgacgcgc
    10801 gcgacggtcg cctgcccctc ctcgccggcg cgtcgacgct cggcgattcg cgcggctcga
    10861 gaacgggcat ccgaggggca tgagcgaggt gcagatcggc gagtctttca tcggccacgg
    10921 cgcagatgcg gcgcacgtca acaccgtgct gggcgagcgc ggcgggcccg tgggcacggc
    10981 gtgggcgacg gcgctcgcca cgccgcgtgc cgggcacgtg ccgttcgtcg cggtcgtgca
    11041 gcccggactc gccgttcagc cgatgacgct gttcgtgaac aaggcgccca tcgagcccgg
    11101 acgacacgcc gacctcacgt ggggcgccgc ccaggcgggc gtcgccgccg gcgttgcgga
    11161 ggccgtggcc gccgggatcg tcgatcgcga ccgcgccggt gagctcctgg tgatcgccgc
    11221 cgtgtgggtg aatcccgcgg ctgaggacga ggacgcggtg ttcgccaaca accgcgaggc
    11281 cacgctcgcg gcgctgcgca acgggcggga cggcgcgccg ctcgccgagg acgtgctggc
    11341 ggcgcggtca gacccctgga accccttctt ccacgcgcgc tgaccgccac ggactcgatg
    11401 aacgccagga atgacaagat ctcgttcgtc gggctgggcc acatgggcgg cccgatgagc
    11461 cgcaggctcg ccgaggccgg cctggacctg acggtgttcg acctcgaccc cgggcgcgcc
    11521 ggacccgcgc tcgcgcgcgg cgcgcgcctc gccggcaccg ttgaggaagc ggcccgaggc
    11581 gcgggagtgt tcatcaccat gctgcccacg cccgccgccg tcgaggacgt catgctcggc
    11641 gccggccggg ccctgcgggc gctgccagcc ggcgcgctgt ggatcgacat gtccacctcg
    11701 tcgccggacg tcgccgggcg cgtgcgcgtg cggggagccc agaacggcct gcgcgcgctg
    11761 gacgcgcccg tggccggcat ggccgcgggc gccgacgccg gcaccctcga gatcttcgtg
    11821 ggcggggccg cggccgacgt ggcgcaggcg cggcccatcc tcgagcatct gggcgacccg
    11881 gagcgcatct tccacgtcgg cggcaacggg gccgggtacg tggtcaagct catgctcaac
    11941 ctgctgtggt tcgaccagct cgtcgccatc gccgaggtgc tcgccatcgg cacgcgcgcc
    12001 ggggtggacc tcgaggtgct ccaccggtct ctggtcgagg gtcccacctc gagccggctg
    12061 ctggcgcgcg acctcctgcc cctgctgcgg gacggggagt acgaggaggg cttctcgatg
    12121 gccctggcca ccaaggacct gcgcctggcc gtggacctcg cgcgctcggc cggcgtgccg
    12181 gccgagctca gcgcggtcgt cgagcagctg ttcacgcggg cacgcgccgt gtacggcgac
    12241 gacgcggggg agatgacgcc ggtgcggctc tacgaggaga ccgccggcgt cctcctgcgc
    12301 ctgccggccg cgggcgcggg ccgggacggt ccgtcgtccg gtcgtgagac cccggacgag
    12361 ggcgacgggg agcgggccgc ctgacggacc gcgcacccgt gcgcgcgtac cgcgcccgcg
    12421 cgttgaccct cggtgggggc gctggtagag tcggagcacg ttgccaccgc agacagcaag
    12481 agacatcgcc cagagctcag agacccaggg acgtccgtgc ccgggtcttt tgtttatttc
    12541 ctatccccct gtcaggagga cggcatggaa gtagagatcg gccgcggaaa gaagggccgc
    12601 agggcctacg gcttcgacga catcgccatc gtgcccagcc ggcgtacccg cgacgccgag
    12661 gatgtggaca tcacctggaa gctcggcgag cacacgctcg agctcccgtg cctggcctcg
    12721 gcgatggacg gcgtggtcga ccccgccttc gccgtcacca tgggcaagct cggcggcctc
    12781 gccgtcctca acctcgaggg cgtgcagacc cgctacgaga acgccgacga gcagctcgac
    12841 cgcatcgcgt cgctccccaa ggagtcggcg acgcgcacca tgcaggagat ctaccaggag
    12901 cccatcaagg ccgagctcgt cgcgaagcgc gtcaaggaga tcaaggacgg cggcgtgctg
    12961 gccgcggcct cgctcacgcc gcagcgcgtg gaggactact acgaggtcgc gctcgaggcc
    13021 ggtctcgacg tgctcgtcat ccagggcacc gtcgtctcgg ccgagcacgt gagcaccagg
    13081 gtcgaaccgc tcgacctcaa gaagttcatc agcgggtgcg ccgtcccggt catcgtgggc
    13141 ggcgtggcca gctacgccac cacgctgcac ctcatgcgca caggcgccgt cggcgtgctc
    13201 gtcggcgtcg gcccgggcca cgcctgcacc acgcgcggcg tgctcggcat cggcgtcccg
    13261 caggcgacgg cgatcgccga cgccgccgcg gcgcgcgtgc agcacctgct cgagaccggg
    13321 gagtactgca acgtcatcgc cgacggcggc atgcgcaccg gcggcgacgt gagcaaggcc
    13381 atcgccctcg gcgccgacgc cgtcatgatc ggctcgccgc tggcctcggc caccgaggcg
    13441 ccggggcgcg gctaccactg gggcatggcc acgttccacc ccgaactgcc gcgcggcacg
    13501 cgcgtcaagg cgggcaacaa ggggacgctg caggagatcc tcctcggccc ggcgcacgag
    13561 aacgacggca cgcgcaacct gttcggcgcg ctgcgcacga gcatggcgac ctgcggctac
    13621 gagtcgctgg tcaagttcca gaaggccgag gtcatggtcg cgccggcgct catgaccgag
    13681 ggcaagaagc tgcagaagga acaagacgtc gg
//
LOCUS       DASUMX010000154        14738 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_2060985, whole genome shotgun sequence.
ACCESSION   DASUMX010000154 DASUMX010000000
VERSION     DASUMX010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 14738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14738
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2060985"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..761)
                     /gene="dhaM"
                     /locus_tag="VFZ86_07430"
     CDS             complement(<1..761)
                     /gene="dhaM"
                     /locus_tag="VFZ86_07430"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: protein motif:HMM:TIGR02364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HEX5642156.1"
                     /translation="MVGIVIVSHSARIAEGVLELAAQMAGPGVRIAAAGGLDEPEGAL
                     GTDAALVLRALEEVWSEDGVLVLMDLGSAVLSAELAVDLLDEERRGRLLLTEAPLVEG
                     AVAAAVAAGLGDPLDAVAAAARAGLAGKAAHLAPGAGEADVRASRHEAAGTGPVTGPA
                     APTARVTVLNAHGLHARPAALLVRTAAGFDAVVTVADATNGRGPVGARSLNGVATLGA
                     RRGDELVVTADGPQAAEALAAISRLADAGFGELEAA"
     gene            complement(764..1447)
                     /gene="dhaL"
                     /locus_tag="VFZ86_07435"
     CDS             complement(764..1447)
                     /gene="dhaL"
                     /locus_tag="VFZ86_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020510648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HEX5642157.1"
                     /translation="MNAPRGTVTTAGAVAWLRAFAAEVAARREQLTELDAAIGDADHG
                     VNMDRGMRAVVAKLPDGAVAADAGAAPTLGALFKTAGMTLVSTVGGAAGPLYGTLFLE
                     MGKAAADRTALSGAEWAGVVGAGVAGVQRRGKAEAGDKTMVDALLPAVESLKAAAGSG
                     ASLGDALRAAADAAHGGMEATVPLVARKGRASYLGERSAGHQDPGATSSWLLLRTAAE
                     TLAGEGDAG"
     gene            complement(1444..2445)
                     /gene="dhaK"
                     /locus_tag="VFZ86_07440"
     CDS             complement(1444..2445)
                     /gene="dhaK"
                     /locus_tag="VFZ86_07440"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HEX5642158.1"
                     /translation="MKKLINRPADVVREELVGMALAHAGRLVVHTSPHWIARADAPVA
                     GKVAIVSGGGSGHEPLHGGFVGPGMLDAACPGEVFTSPTPDQVLAATQAVDGGAGVLH
                     IVKNYTGDVMNFEMAADLARDEGLVVEQVVIADDVAVEDSLYTAGRRGVGATVLAEKI
                     CGAAAEEGRPLAEVAGLCRTVNELSRSMGVALTSCTTPMAGKPTIDLDAFHMEVGVGI
                     HGEPGRKRALAASAHAIVSELAAAVVDDLPFSSGDKVLAFVNGMGGTPLMELYIVYGE
                     LHDFLEDRGIVIARNLIGSYATSLDMAGCSITLLRLDDELTRLWDAPVDTPGLRWGA"
     gene            complement(2573..3061)
                     /locus_tag="VFZ86_07445"
     CDS             complement(2573..3061)
                     /locus_tag="VFZ86_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEX5642159.1"
                     /translation="MSWVAVHARHARGVYATDAPGTPDYCRRLPHPEDTIDVKNEELL
                     PLFAEVKQLLTPYAARLTVRRNEPGYYDLWSEKEIVVSGRRRKEVFFAGAIVQKSFVG
                     FYFMPLYADDDLSRVFGPELLATLKGKSCFHLKRMTPELRAQIETALAAGWRLYEERG
                     WV"
     gene            3080..3328
                     /locus_tag="VFZ86_07450"
     CDS             3080..3328
                     /locus_tag="VFZ86_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642160.1"
                     /translation="MSRTAKAYRLVPADVPPPAGRGAGLYAEIVADFVSSGVASALVE
                     MPGRKANSMNVGLRKAVAASGAGVKVVIRAGQVYLKKT"
     gene            3397..4779
                     /locus_tag="VFZ86_07455"
     CDS             3397..4779
                     /locus_tag="VFZ86_07455"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5642161.1"
                     /translation="MTATSPTPARAGPLQRTFRSLHVYNYRLFFFSQVISMSGTWMQS
                     VAQNWLVLSLTGSAVDLGITVGLQFGPVLLFGAWAGALADRVDKRKLLMGTQAAAAVL
                     ALVLGVLAATDVVTVWMIWVLAGLTGTATAMGIPSQQSFLYEMVGPDDVANAVGLNSV
                     VINSSRIIGPAIGAVLIAGVGVAPCFFINGASFLAVIGALAIMRTAELRRGKPLAREP
                     GQVRAGLRYAWSTPALRVPLVMLAVISILAYNYSVVLPLLTKSEFGRGGGSYGVMSAA
                     MGVGALAGALLMASRARPSRRLLVGAAFAFGVVTIALSLAPGYYVGLGLLVLMGGAGV
                     FFISTTNAMLQVNAANAMRGRVMALWSIVFLGSTPIGGPITGLLVRGLGVRWAIAIGG
                     IAAIATATGAVIALRHRRVVEEGSCEAPACLPDGPAPGDALGEAVKVGGGSSAGRPPD
                     AAPAPAAGRR"
     gene            4877..6163
                     /locus_tag="VFZ86_07460"
     CDS             4877..6163
                     /locus_tag="VFZ86_07460"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HEX5642162.1"
                     /translation="MLLQGNLQEFSLPNIFQLVKMSAKSGSLSIRRDGEWGRIFFRNG
                     MISYAYSSPQLLPLGERLVKSGAISKTQLKQALAAQKKSPDGPRLGHILLEQGRIDRP
                     TLEQAVREQIQDTAFTFFSWADGEFEFGAEESPPEEDILVEMSVENVIMEGCRRIDEW
                     ELIFEQLGSLERVPHLACGDHVDDEGSLTLTAEEWRVIVHIDGRADINTILHECGLDR
                     FHGAKVMYSLFSSGLISVSEPVIESIGKGTSVAVRGPIDIYNEVFISTLTDSNTVKQL
                     RVELIDEKEVEIPVVAGQMPAQGNGHGGGEGEGAGPAEDVLVFTAASTCPEQGWRRLA
                     GESSAWVVLANANDADSLRSTRADLQFIRTLDDVPYVVATYVSMADDELSPKQIARAL
                     GLDAGTPVLACQLRDRESVAGVVRAALDLAAARRGA"
     gene            6294..7265
                     /gene="hemB"
                     /locus_tag="VFZ86_07465"
     CDS             6294..7265
                     /gene="hemB"
                     /locus_tag="VFZ86_07465"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEX5642163.1"
                     /translation="MYYPSYRMRRLRRTPAIRDLVRETRLQLDDLVYPLFVIAGENVK
                     NPISSMPGCYQLSIGNVLPEVREVVRLGIKAILLFGIPAHKDPAATGAYDHEGVVQMA
                     IRAIKDEFPELVVITDVCLCEYTDHGHCGVVQEGEVLNDVTLELLAKMAITHAESGAD
                     IVAPSDMMDGRVAAIRNALDDDGLTDTILMSYSSKFASAFYGPFREAAESAPAFGDRK
                     TYQMDPANLEEAVRESLLDIEEGADMIMVKPAMPFLDVIHAVKQETKFPLAAYSVSGE
                     YAMIKAAAANGWLDEQRAVLEAVTGIKRAGADLIITYHAKDIAAWLG"
     gene            7473..8585
                     /gene="ahbD"
                     /locus_tag="VFZ86_07470"
     CDS             7473..8585
                     /gene="ahbD"
                     /locus_tag="VFZ86_07470"
                     /EC_number="1.3.98.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015948593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006785 - heme B biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme b synthase"
                     /protein_id="HEX5642164.1"
                     /translation="MNARPGEAEGASGPAPPRVIAWEVTRSCNLACVHCRASAVHGPY
                     EGELSTDEAFTLVDQIASFANPILILTGGDPLMRADIFDIAGHAIAKGLRTVMSPNGT
                     LVTPEVARRMRDVGIARVSISIDFPNAAEHDAFRGVPGAFDGAVRGVRNCLAAGVEVQ
                     INSTITKLNAHYLPDLLKLATDLGAVSFHPFMLVPTGRGKELEEQELPPDEYEKALEW
                     MYDAQQESDLFMKPTDVPHYWRVMRQRAKAQGRKLEVHPHSHGGMNTLSRGCLAGIGF
                     CFISHIGEVQGCGYFDKVAGDVRTQTFPEVWRDSPLFNELRDFDGLKGKCGVCEFKRV
                     CGGCRARAYEATGDYMAEEPYCAYVPKGWCIPAAGI"
     gene            complement(8609..9145)
                     /locus_tag="VFZ86_07475"
     CDS             complement(8609..9145)
                     /locus_tag="VFZ86_07475"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5642165.1"
                     /translation="MPVDLRPPAAGDEAFLHEMLREADRWRLPIDAPRPPLAEILADP
                     HVRVYVEGWGRPGDDGIVAEVAGSRVGACWLRLFGEESHGWGFVGPDVPELSIAVAPR
                     WRRRGIGRRLLAAAVARAEQLGHPALSLSVMPENPARELYLQAGFRRVAVVDGSWTMT
                     LRLPRDAAGRRSPVPARP"
     gene            complement(9145..9606)
                     /locus_tag="VFZ86_07480"
     CDS             complement(9145..9606)
                     /locus_tag="VFZ86_07480"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5642166.1"
                     /translation="MTITIRQAGPGDEAAIVRLTQEMAAGEDDVSPVDEHYAHRFLAS
                     PAAGVLLAEDDGEAVGLLSYALVPGLYHAADSGLIELLIVTGTRRGEGIGHRLVRAAL
                     RAFHEAGCAEASVSTGEENAGAQRVYRDAGLTESSLLLETHFDSAGSKSRT"
     gene            9719..11011
                     /gene="hemL"
                     /locus_tag="VFZ86_07485"
     CDS             9719..11011
                     /gene="hemL"
                     /locus_tag="VFZ86_07485"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEX5642167.1"
                     /translation="MNTETSARLFQEALQYIPGGVNSPVRAMKGVGMPHPLFIARADR
                     SHIWDADGNEFVDFVSSWGPMILGWGHPEVVGRLKEQVDRGTSYGAPTELEVELARLV
                     TEAVPSVELVRMVNSGTEATMSAVRVARGFTGREKIVKFVGCYHGHSDGFLAAAGSGV
                     LTLALPDSPGVTRATTADTLLVEYNDVQTLKELFAAEGDAIAALIVEPVAGNMGVVPP
                     QPGFLEACRDLCTRYGAVLIFDEVITGFRVAWGGAQERYGVLPDLTTLGKIIGGGLPV
                     GAFGGRRDIMETVAPLGSTYQAGTLSGNPLAMAAGAATLRVLAEPGTYERLEASGARV
                     EEALRAAAGAAGRAVTVNRVGPMLTMFFCEGPVHSFADAKAADTGAYAAYWRHMLERG
                     VYLAPSQFEAAMISLALTDADLEKAAGAASDWFAGAGA"
     gene            11013..11663
                     /locus_tag="VFZ86_07490"
     CDS             11013..11663
                     /locus_tag="VFZ86_07490"
                     /inference="COORDINATES: protein motif:HMM:NF003989.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEX5642168.1"
                     /translation="MRGIDKNDVPSVTVPRLALYLRKLRELRARGVERVSSKDLAEMI
                     DLNAAQIRKDFSYFGEFGTRGVGYEVPVLVDEITRCLGLDHTWNVVIVGAGLLGTALA
                     RYRGFGEQGFRLVGMFDSSATVIGARFGSDEADRVRSIHELDEFCRKERVDIAMVTVP
                     AGEAESTVQRLGSSGVKAILNFAPVKVHAPEGVLVRQVDLSSELMSLSFYLDKERD"
     gene            11716..12315
                     /locus_tag="VFZ86_07495"
     CDS             11716..12315
                     /locus_tag="VFZ86_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642169.1"
                     /translation="MDVLSTDLAAGVAAALAVTGKAFGPPPAAAPEPRWGALYEELDP
                     AGDVAFAVDIELIYEGYLLHYRESRALSLAAGDLETRLLAGDCFYARGLRGVAARGDA
                     DAVGLLARLMAACSFLRAAAAPFAGDDALWAYTTAGVAAQHAGAAPASVAALFDHIEA
                     GFHRERLPDVTAAVAGAVARLSLPDPAPLLRELEVVAGR"
     gene            12312..13754
                     /locus_tag="VFZ86_07500"
     CDS             12312..13754
                     /locus_tag="VFZ86_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HEX5642170.1"
                     /translation="MTVDLRAFISLLDREGELARVRPEVDPYLEMAEIADRASKAHGP
                     AVLFEQPKRPAGQPAVPVLMNQLGSYRRLELALGAPLDDIAGRIGGLLELQVPSGLVG
                     KVKALGQLKELASYGPRYGKKALWREVVVDPPDLARLPVLTTWPGDGGPFITLPIVVT
                     KDRQGRQNAGMYRLQVFDGQTTGMHIHIHHDGAANFREAGDRMEVAVALGTDPAVTYA
                     ATAPLPPGISEFMFAGFLRGEPVDMAQCATVDLQVPADAEIVLEGYVEKGETRLEGPF
                     GDHTGYYSLADQYPLFHLTAMSHRRDPIYPATIVGKPPMEDAYLGKATERLFLPLLQL
                     VQPEIVDMDLPIEGVFHDCAIISIRKEYPGHARKIMNAVWGMGQMMFTKFVVVVDEHV
                     DVHDYSEVTWRVFNNVDPERDTVIVRGPLDVLDHSSPFARYGAKMGIDATKTWPDEGH
                     GREWPEELAMDPAVVRRVDERWKEFGLPFS"
     gene            13896..>14738
                     /locus_tag="VFZ86_07505"
     CDS             13896..>14738
                     /locus_tag="VFZ86_07505"
                     /inference="COORDINATES: protein motif:HMM:NF015237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HEX5642171.1"
                     /translation="MTERELRHRPATRPWYRRAWFTWIVVIAIAVVVVAILVFPVAAM
                     DDQSYCTSCKAMQPAEKTLAASAHAGIDCTKCHIPPGVVPAARWRLAEAKNVWADYLG
                     MPSTAEKEHVPTNANCLECHPLSGIPNESDGVRMNHEEHLKLRNLLCVDCHDTVSHKL
                     PGQTDGVSMVTCSMCHNEQGAPDACDYSHPAPPASEHAPDFMKEHGKEALVDEEACLR
                     CHHDKPAFCDDCHAYPPSTHYSGQWRYTHGEDAEADPANCEACHDEAYCSQCHQVNHP
                     NGWLQ"
BASE COUNT         2120 a         5496 c         5042 g         2080 t
ORIGIN      
        1 gcggcctcaa gctccccgaa ccccgcgtcg gcgaggcggc tgatcgcggc cagcgcctcc
       61 gccgcctgcg gcccatcggc cgtgacgacg agctcgtcgc cacggcgcgc ccccagggtc
      121 gcgacgccgt tgaggctgcg cgccccgacg ggtccgcggc cgttggtcgc gtccgcgacg
      181 gtgaccacgg cgtcgaagcc tgccgcggtg cgcaccagca gcgcggccgg ccgtgcgtgg
      241 agcccatgcg cgttgaggac cgtcacgcgg gcggtcggcg ccgccgggcc ggtcaccgga
      301 cccgtgccgg cggcctcatg ccggctcgcg cgcacgtctg cctcgcccgc gcccggcgcc
      361 aggtgggccg ccttgccggc gagcccggcg cgagccgcgg cggccacggc gtccagcggg
      421 tcgcccagcc cggcggcgac ggccgcggcc acggcgccct ccacgagcgg cgcctccgtg
      481 agcaggagcc tgccgcgccg ctcctcgtcc agcaggtcga cggccagctc ggcggagagg
      541 acggcgctcc ccaggtccat caggaccagc acgccgtcct ccgaccacac ctcctcgagc
      601 gctcgcagca cgagcgcggc gtccgtgccg agcgccccct ccggctcgtc cagccctccg
      661 gcggccgcga tccgcacgcc gggaccggcc atctgcgcgg ccagctccag gacgccttcc
      721 gcgatccggg cgctgtggct gacgatgacg atgccgacca cggctagccc gcgtcaccct
      781 cgccggccag cgtctcggcc gccgtccgca ggagcagcca cgacgacgtc gcgcccgggt
      841 cctgatgccc ggcgctgcgc tcgcccaggt agctggcgcg ccccttgcgc gccacgagcg
      901 gcacggtcgc ctccatgccg ccgtgggcgg cgtccgcggc cgcccgcagc gcgtcgccga
      961 gcgacgcgcc ggagccggcc gcggccttca gggactccac ggccggcagc agcgcgtcca
     1021 ccatcgtctt gtctcccgcc tcggccttgc cgcgtctctg caccccggcc acccccgcgc
     1081 cgacgacgcc ggcccactcg gccccggaga gggcggtcct gtccgcggcg gctttgccca
     1141 tctccaggaa cagcgtcccg tagagcggtc ccgcggcgcc gccgacggtc gagacgaggg
     1201 tcatcccggc ggtcttgaac agcgcgccga gggtgggcgc cgcgccggcg tcagcggcga
     1261 ccgccccgtc cggcagcttc gcgaccaccg ctcgcatccc gcggtccatg ttgacaccgt
     1321 ggtccgcgtc cccgatggcc gcgtcgagct ccgtgagctg ctcgcgccgc gccgccacct
     1381 cggccgcgaa cgcccgcagc cacgcgaccg cccccgccgt cgtgaccgtc ccgcgcggcg
     1441 cgttcacgcc ccccaccgca ggccgggggt gtcgaccggg gcgtcccaca gccgcgtcag
     1501 ctcgtcgtcc agccgcagga gtgttatcga gcagcccgcc atgtcgaggg acgtggcata
     1561 ggagccgatc aggttgcgcg cgatgacgat gccgcggtcc tcgaggaagt cgtggagctc
     1621 gccgtagacg atgtagagct ccatgagcgg cgtgccgccc atgccgttga cgaacgcgag
     1681 caccttgtcg ccgctcgaga acgggaggtc gtccacgacc gcggcggcca gctcggagac
     1741 gatggcgtgg gccgaggccg ccagcgcgcg cttgcgcccg ggctccccgt ggatgccgac
     1801 gccgacctcc atgtggaacg cgtcgaggtc gatcgtcggc ttgccggcca tgggcgtggt
     1861 gcacgaggtg agcgccacgc ccatgctgcg gctgagctcg ttcaccgtgc gacagaggcc
     1921 ggccacctcc gcgagcggcc ggccctcctc ggccgcagcg ccgcagatct tctcggccag
     1981 caccgtggcg cccacgccgc ggcgtccggc ggtgtacagg ctgtcctcga cggccacgtc
     2041 gtcggcgatc acgacctgct cgaccacgag cccctcgtcg cgggccaggt cggcggccat
     2101 ctcgaagttc atgacgtcgc cggtgtagtt cttgacgatg tgcagcacgc cggcgccgcc
     2161 gtcgacggcc tgcgtcgccg ccagcacctg gtcgggggtc ggcgaggtga agacttcgcc
     2221 cgggcaggcg gcgtcgagca tgcccgggcc gacgaacccc ccgtgcagcg gctcgtgccc
     2281 cgaccctccg ccggagacga tcgccacctt gcccgccacg ggcgcgtcgg cgcgcgctat
     2341 ccagtgcggg ctggtgtgga cgacgagacg gccggcgtgc gcgagcgcca tgccgacgag
     2401 ctcctcccga acgacgtccg caggacggtt gatgagtttc ttcatcgcgc tcccctccag
     2461 ctacggccgc cggcacggtc cggcggcgtc atggtcctcg cgaccatcgt tcgccgcgcg
     2521 cgcgccgccg tcaaggcgcg gggagagttc cgtccggcgc ggacggccgg gctcacaccc
     2581 agccgcgctc ttcgtagagg cgccagcccg ccgcgagcgc ggtctcgatc tgcgccctga
     2641 gctcgggcgt catgcgtttg aggtggaagc acgacttgcc cttgagcgtc gccagcagct
     2701 cggggccgaa cacgcgggag aggtcgtcgt ctgcatacaa cggcatgaag tagaagccga
     2761 cgaagctctt ctggacgatg gcgccggcga agaagacctc cttgcgtcgc cggccggaga
     2821 cgacgatctc cttctccgac cacaggtcgt agtagccggg ctcgttcctg cgcaccgtca
     2881 accgagccgc atacggagtg agcaactgct tgacctccgc gaacaacggg aggagttcct
     2941 cgttcttgac gtcgatggtg tcctccggat gcggaagtcg cctgcagtag tctggtgtac
     3001 ccggcgcgtc ggtggcgtag actccccgtg catgtcgcgc gtgaaccgcg acccaagaca
     3061 ccagatggag gtgccgccca tgtcccgcac cgcgaaggcc tatcgactcg tgcccgccga
     3121 cgtaccgccg ccggccggga gaggcgccgg gctgtacgcc gagatcgtcg ccgacttcgt
     3181 ctcctccggc gtcgcgtcgg cgctcgtcga gatgccgggc cgcaaggcca actccatgaa
     3241 cgtcggcctg cgcaaggccg tggctgcgtc cggcgccggc gtcaaggtcg tcatccgcgc
     3301 cggccaggtg tacctgaaga agacctgagg gcggcacgcg ccggtaggcc ttccccctcg
     3361 gaggacaccc gcgcggcgcg gcggtagact caggcgatga ccgcgacttc ccccactccg
     3421 gcgcgcgccg gcccgctgca gcgcaccttc cgctcgctcc acgtgtacaa ctaccggctg
     3481 ttcttcttca gccaggtgat ctcgatgagc ggcacctgga tgcagtcggt ggcgcagaac
     3541 tggctcgtgc tcagcctcac cggcagcgcc gtcgacctcg gcatcaccgt ggggctccag
     3601 ttcgggccgg tgctcctgtt cggcgcgtgg gcgggggcgc tggccgaccg cgtggacaag
     3661 cgcaagctct tgatggggac gcaggccgcg gccgcggtcc tcgcgctcgt gctcggcgtc
     3721 ctcgccgcca cggatgtggt gacggtgtgg atgatctggg tgctcgccgg cctcaccggc
     3781 acggccacgg ccatgggcat cccctcacag cagagcttcc tgtacgagat ggtcggcccc
     3841 gacgacgtcg ccaacgcggt cggcctcaac tcggtggtca tcaactcctc gcgcatcatc
     3901 ggcccggcca tcggcgccgt gctgatcgcc ggcgtcggcg tcgccccctg cttcttcatc
     3961 aacggcgcgt cgttcctggc cgtcatcggc gcgctggcga tcatgcgcac cgccgagctg
     4021 cgccgcggca agccgctggc gcgcgagccc ggtcaggtgc gcgcggggct gaggtacgcc
     4081 tggagcacgc cggctcttcg cgtgccgctc gtcatgctcg ccgtcatcag catcctcgcc
     4141 tacaactact ccgtcgtgct gccgctcctc accaagtcgg agttcggccg cggcggcggc
     4201 tcctacggag tcatgtccgc cgccatgggc gtcggcgcgc tcgccggcgc cctgctcatg
     4261 gcgagcaggg cgcgcccctc gcgccggctg ctcgtgggcg cggccttcgc tttcggcgtc
     4321 gtgaccatcg ccctgtcgct tgctcccggc tattacgtgg ggctcgggct cctcgtcctc
     4381 atgggcggcg cgggcgtgtt cttcatcagt acgaccaacg ccatgctgca ggtcaacgcg
     4441 gcgaacgcga tgcgcggccg cgtcatggcg ctctggtcga tcgtcttcct cgggagcacg
     4501 cccatcggag gacccatcac cgggctcctc gtgcgcggcc tcggcgtgcg ctgggcgatc
     4561 gccatcggcg gcatcgccgc catcgccacg gccaccggcg ccgtcatcgc cctgcgtcac
     4621 cgccgcgtgg tcgaggaggg cagctgcgag gcgccggcct gcctgccgga cgggccggcg
     4681 ccgggcgacg ctctgggcga agccgtgaag gtgggcggcg gctcgtccgc cggccgcccc
     4741 cccgacgcgg ctcccgcccc cgccgccggc aggcggtagc ggacctcaag tcggcggcgg
     4801 cgtcgccgat gtaggtcacg gggccgcgcg acgggcgcgg ccctccccat gaggcggcgg
     4861 cgaggaaagg agagcggtgc ttctccaggg caacctgcag gagttcagtc tccccaacat
     4921 cttccagctg gtgaagatga gcgccaagag cggctccctc agcatccgcc gtgacggcga
     4981 gtggggcagg atcttcttcc gcaacgggat gatctcgtac gcctattcct cgccgcagct
     5041 gctcccgctc ggcgagcggc tcgtcaagtc cggcgcgatc agcaagaccc agctcaagca
     5101 ggcgctcgcc gcccagaaga agtccccgga cggcccccgc ctcgggcaca tcctgctgga
     5161 gcaggggcgc atcgacaggc cgaccctcga acaggcggtg cgggagcaga tccaggacac
     5221 cgccttcacc ttcttcagct gggccgacgg cgagttcgag ttcggcgccg aggagagccc
     5281 tccggaggag gacatcctcg tcgagatgag cgtcgagaac gtcatcatgg agggctgccg
     5341 ccgcatcgac gagtgggagc tgatcttcga gcagctgggg tcgctggagc gtgtgccgca
     5401 cctcgcctgc ggcgaccacg tcgacgacga gggcagcctg acgctcaccg cggaggagtg
     5461 gcgcgtgatc gtccacatcg acggccgcgc cgacatcaac accatcctcc acgagtgcgg
     5521 cctggaccgc tttcacggcg ccaaggtcat gtacagcctg ttctcgagcg ggctcatcag
     5581 cgtcagcgaa ccggtcatcg agagcatcgg caagggcacg agcgtcgccg tgcgcgggcc
     5641 catcgacatc tacaacgagg tcttcatcag cacgctcacg gactccaaca ccgtcaagca
     5701 actccgcgtg gagctgatcg acgagaagga ggtcgagatc ccggtggtgg ccgggcagat
     5761 gccggcgcag ggcaacggcc acggcggggg agagggcgag ggcgccggcc ctgccgaaga
     5821 cgtcctcgtc ttcaccgccg cctccacctg cccggagcag ggctggcggc ggctcgccgg
     5881 cgagagctcc gcctgggtgg tgctcgccaa cgccaacgac gccgacagcc tgcgctccac
     5941 gcgcgccgac ctgcagttca tccgcacgct cgacgacgtg ccctacgtgg tggccaccta
     6001 cgtctcgatg gccgacgacg agctcagccc gaagcagatc gccagggcgc tcggtctcga
     6061 cgccgggacg ccggtgctgg cctgccagct gcgcgaccgg gagtcggtcg ccggcgtcgt
     6121 cagagcggcg ctcgatctgg ccgccgcgcg ccgcggcgcc tgatccggcg ctgcgtccgg
     6181 ccggcgccgc cgcgccaacg ccgccccgcg cttgtcatcc tcgcgtcacg gcgcgaagat
     6241 tgaccgccta ggcgggccgc gcccgggccc gcgcgaagaa aggacaccac cgcatgtact
     6301 accccagcta ccgcatgagg cgcctgcgcc gcacccccgc catccgggat ctggtgcgcg
     6361 agaccaggct gcagctcgac gacctggtct acccgctgtt cgtcatcgcc ggcgagaacg
     6421 tcaagaaccc catctcgtcg atgcccggct gctaccagct ctccatcggc aacgtgctgc
     6481 ccgaggtgcg cgaggtggtc aggctcggca tcaaggcgat cctgctgttc ggcatcccgg
     6541 cgcacaagga tccggccgcc accggcgcct acgaccacga gggcgtcgtg cagatggcca
     6601 tccgcgcgat caaggacgag ttcccggagc tggtggtcat caccgacgtg tgcctctgcg
     6661 agtacaccga tcacgggcac tgcggcgtcg tccaggaggg tgaggtgctc aacgacgtca
     6721 ccctcgagct gctcgccaag atggcgatca cgcacgccga gagcggcgcc gacatcgtcg
     6781 ctccgagcga catgatggac ggccgcgtcg ccgccatccg caacgcgctc gacgacgacg
     6841 gcctgacgga caccatcctc atgtcgtact cgagcaagtt cgcctcggcg ttctacggcc
     6901 cgttccgaga ggccgccgaa tccgcccccg cgttcggcga ccgcaagacg taccagatgg
     6961 acccggccaa cctcgaggag gccgtgcgcg agtccctgct cgacatcgag gagggcgccg
     7021 acatgatcat ggtgaagccg gcgatgccct tcctcgacgt catccacgca gtcaagcagg
     7081 agaccaagtt cccgctcgcc gcctacagcg tgagcggcga gtacgcgatg atcaaggcgg
     7141 cggcggccaa cggctggctc gacgagcagc gcgccgtgct cgaggccgtc accggcatca
     7201 agcgcgccgg cgccgacctc atcatcacct accacgccaa ggacatcgcc gcctggctcg
     7261 gatagccgcc gcgggcgggc cggcggcggc ggcgcgaaga cgcgccggtc actggctatc
     7321 gggctcccgg cgctgtaagg tggcagtccg tccctcgcgg gcggctgcag gagagggtcg
     7381 ggcggcgcac ccatctgcgc gttgcgccgc ccgcccgtct ctggcacact ggacacggtt
     7441 tgcaccatcc tcacgctgaa aacccccatg gtatgaacgc gcgacccggc gaagccgagg
     7501 gcgcatccgg cccggcgccg ccgcgcgtca tcgcctggga ggtcacgcgc agctgcaatc
     7561 tggcctgcgt gcactgccgt gcctcggcgg tccacggacc ctacgagggc gagctcagca
     7621 ccgacgaggc cttcaccctc gtggaccaga tcgcctcgtt cgcgaacccg atcctcatcc
     7681 tcaccggcgg cgacccgctc atgcgcgccg acatcttcga catcgccggg cacgccatcg
     7741 ccaagggcct gcgcaccgtc atgtcgccca acggcacgct ggtcacgccc gaggtcgcgc
     7801 ggcgcatgcg cgacgtcggc atcgcccgcg tcagcatctc catcgacttc ccgaacgccg
     7861 cggagcacga cgcgttccgc ggcgtccccg gcgccttcga cggcgccgtc cgcggcgtcc
     7921 gcaactgcct cgccgcgggc gtcgaggtgc agatcaacag caccatcacc aagctcaacg
     7981 cgcactacct tcccgacctt ctcaagctcg ccaccgacct gggcgccgtc tcgttccacc
     8041 ccttcatgct ggtgcccacg gggcgcggca aggagctcga ggagcaggag ctgccgccgg
     8101 acgagtacga gaaggcgctc gagtggatgt acgacgcgca gcaggagagc gacctgttca
     8161 tgaagcccac cgacgtgccg cactactggc gcgtcatgcg ccagcgcgcc aaggcgcagg
     8221 gccgcaagct cgaggtgcac ccgcacagcc acggcggcat gaacaccctc agccgcggct
     8281 gcctcgccgg catcggtttc tgcttcatca gccacatcgg cgaggtgcag ggctgcggct
     8341 acttcgacaa ggtcgccggc gacgtccgca cgcagacgtt ccccgaggtc tggcgcgact
     8401 caccgctctt caacgagctc cgcgacttcg acggactcaa gggcaagtgc ggcgtctgcg
     8461 agttcaagag ggtctgcggc ggctgccgcg cgcgggccta cgaggcgacc ggcgactaca
     8521 tggccgaaga gccctactgc gcctacgtcc ccaagggctg gtgcatcccg gccgccggga
     8581 tctgagcccg cttcctcctt cgtgagcctc aggggcgcgc cggaaccggt gaccttcggc
     8641 cggcggcgtc ccgcgggagt cgcagcgtca tcgtccacga gccgtcgacg accgcgaccc
     8701 gccggaaccc cgcctgcagg tacagctcgc gggccgggtt ctcaggcatg acgctcaggc
     8761 tgagcgcggg atggccgagc tgctccgcgc gggccacggc ggcggcgagg aggcgccggc
     8821 ctatcccccg gcgccgccac cgcggggcga cggcgatact gagctcgggc acgtccggtc
     8881 cgacgaaccc ccagccgtgc gactcttcgc cgaacagccg cagccagcag gctccgacac
     8941 gggagccggc cacttcggcg acgataccgt cgtcgccggg gcgtccccac ccctccacgt
     9001 agacgcgcac gtgcgggtcg gcgaggatct ccgccagcgg cggccgagga gcgtcgatcg
     9061 gcaggcgcca gcggtccgcc tctcgcagca tctcgtgcag aaacgcttcg tccccggccg
     9121 ccggcggccg gaggtccacc ggcatcacgt ccgcgacttg gatccggcgg agtcgaagtg
     9181 cgtctcgagc agcagggacg actcggtgag tcccgcgtcg cggtagaccc tctgcgcgcc
     9241 ggcgttctcc tcgccggtgc tgacggacgc ctcggcgcag ccggcctcgt ggaacgcgcg
     9301 cagggcggcg cggacgagcc ggtgtccgat gccctcgccg cggcgcgtgc ccgtgacgat
     9361 cagcagttcg atgaggccgg agtcggcggc gtggtacagc ccgggcacga gcgcgtagct
     9421 gagcagcccc accgcctcgc cgtcgtcctc ggccagcagc acgccggcgg ccggcgacgc
     9481 gaggaagcgg tgcgcgtagt gctcgtctac gggggagacg tcgtcctcgc cggccgccat
     9541 ctcctgcgtg agacgcacga tggcggcctc gtctccgggc cccgcctgtc ggatcgtgat
     9601 cgtcatgcct gcttcccccc cgaatctggg taggtcgtgt gtcgaggcac atgcgcgccg
     9661 gcgcgcgtag tatagtgcca gctttgcaca ggctttctca ctcgcaggga ggacccgggt
     9721 gaacaccgag acgtccgcgc gcctgttcca ggaggcgctc cagtacatcc ccggcggggt
     9781 caactcgccc gttcgcgcga tgaagggtgt cggcatgccg caccccctgt tcatcgcgcg
     9841 cgccgaccgc tcccacatct gggacgccga cggcaacgag ttcgtcgatt tcgtctcgtc
     9901 ctggggtccg atgatcctgg gctgggggca ccccgaggtc gtcggccgtc tcaaggagca
     9961 ggtggaccgc ggcacgagct acggcgcgcc caccgagctg gaggtcgagc tggcgcgcct
    10021 ggtcaccgag gcggttccgt cggtggagct cgtgcgcatg gtcaactcgg gcaccgaggc
    10081 gacgatgagc gccgtgcgcg tcgcccgcgg gttcaccggc cgcgagaaga tcgtcaagtt
    10141 cgtcggctgc taccacggcc attccgacgg cttcctggcc gcggccggca gcggcgtgct
    10201 cacgctcgcg ctgccagaca gtcccggcgt caccagggcg acgacggccg acacgctgct
    10261 cgtcgagtac aacgacgtgc agacgctcaa agagctgttc gcggccgagg gcgacgccat
    10321 cgccgcgctc atcgtcgagc cggtggccgg caacatgggc gtggtgccgc cgcagccggg
    10381 cttcctcgag gcctgccgcg acctgtgcac gaggtacggc gccgtgctca tcttcgacga
    10441 ggtcatcacc ggcttccgcg tcgcctgggg cggcgctcag gagcgctacg gcgtcctgcc
    10501 ggacctcacc acgctcggca agatcatcgg cggcggcctc ccggtcggcg ccttcggcgg
    10561 ccgccgcgac atcatggaga cggtggcccc cctgggctcg acctaccagg ccgggacgct
    10621 ctcgggcaac ccgctcgcca tggccgccgg cgcggcgacg ctgcgcgtgc tcgccgagcc
    10681 ggggacgtac gagcgcctcg aggccagcgg cgctcgcgtc gaggaggcgc tgcgcgcggc
    10741 cgccggcgct gcgggccgcg ccgtcaccgt caaccgggtc gggcccatgc tcacgatgtt
    10801 cttctgcgag ggtcccgtgc acagcttcgc cgacgccaag gcggccgaca ccggcgccta
    10861 cgcggcgtac tggcggcaca tgctcgagcg cggcgtgtac ctcgcgccgt cgcagttcga
    10921 ggcggccatg atctcactgg cgctcaccga cgcggatctc gagaaggctg ccggcgcggc
    10981 gagcgactgg ttcgcagggg cgggggcctg aggtgcgcgg catcgacaag aacgacgtcc
    11041 ccagcgtcac cgtcccgcgg ctcgcgctgt acctgcgcaa gctgcgcgag ctccgggcgc
    11101 gcggcgtcga gagggtctcc tccaaggatc tcgcggagat gatcgacctg aacgccgcgc
    11161 agatccgcaa ggacttctcg tacttcggcg agttcggcac gcgcggcgtg ggctacgagg
    11221 tgccggtgct ggtcgacgag atcacccgct gtctcggtct cgatcacacc tggaacgtcg
    11281 tcatcgtcgg cgccggcctc ctgggcaccg cgctggcgcg ctaccgcggg ttcggcgagc
    11341 agggcttccg cctcgtcggc atgttcgaca gctcggcgac ggtcatcggc gcccgcttcg
    11401 ggtccgacga ggccgaccgc gtgcgcagca tccacgagct ggacgagttc tgccgcaagg
    11461 aacgggtcga catcgctatg gtcaccgttc ccgccggcga ggccgagagc acggtgcagc
    11521 ggctcgggtc atccggcgtc aaggccatcc tcaacttcgc gccggtaaag gtccacgcac
    11581 ccgagggtgt gctggtccgt caggtcgacc tctccagcga gctgatgtcc ctgtcgttct
    11641 acctcgacaa ggaacgcgat tgatcgccgc aggcaccgta gtgccggtga gcggctcgcg
    11701 gccgaagcga tcacggtgga cgtcctgagc acggatctcg ccgccggcgt cgccgcggcg
    11761 ctggcggtga ccgggaaggc cttcggcccg ccgccggccg ccgcccccga gccccggtgg
    11821 ggcgcgctct acgaggagct cgaccctgcc ggggacgtcg cgttcgcggt cgacatcgag
    11881 ctcatctacg aggggtacct gctgcactac cgcgagagcc gcgccctttc actcgcggcc
    11941 ggcgacctcg agacccggct gctggccggt gactgcttct acgcacgcgg cctgcgcggt
    12001 gtcgccgcgc gcggggacgc cgacgccgtc ggccttctcg cgcgactcat ggccgcctgt
    12061 tcgttccttc gtgcggccgc cgcgccgttc gccggcgacg acgccctctg ggcgtacacg
    12121 acggccggcg tcgcggcgca gcacgccggg gcggccccgg cgtcggtcgc ggcgctgttc
    12181 gaccacatcg aagccggctt ccaccgggag cgcctgccgg acgtgaccgc cgcggtggcc
    12241 ggcgccgtcg cgcgtctctc tctgccggac cccgccccgc tgctgcggga gctcgaagtc
    12301 gtcgccggga ggtgaccgtg gacctgcgtg ccttcatcag cctgctggac cgcgagggcg
    12361 agttggcccg cgtgcgcccg gaggtcgacc cgtatctcga gatggcggag atcgccgacc
    12421 gcgccagcaa ggcgcacggc ccggcggtcc tgttcgagca gcccaagcga ccggcagggc
    12481 aacccgccgt gccggtcctc atgaaccagc tcggctcgta ccggcgtctc gagttggcct
    12541 tgggcgcgcc gctggacgac atcgccgggc gcatcggcgg cctgctcgag ttgcaggtgc
    12601 cgagcggcct cgtcggcaag gtcaaggcgc tcggccagct caaggagctc gcctcctacg
    12661 ggccgcgcta cggcaagaag gcgttgtggc gcgaggtcgt cgtcgacccg ccggacctcg
    12721 cgcgcctgcc ggtgctcacc acctggcccg gcgacggcgg cccgttcatc accctgccga
    12781 tcgtggtcac caaggaccgg cagggccgcc agaacgccgg catgtaccgg ctgcaggtgt
    12841 tcgacggcca gaccaccggc atgcacatcc acatccacca cgacggcgcg gccaacttcc
    12901 gcgaggcggg cgaccgcatg gaggtggcgg tcgccctcgg caccgacccc gccgtcacgt
    12961 atgccgccac ggcgccgctc ccgccgggca tcagcgagtt catgttcgcc ggcttcctcc
    13021 gcggagagcc ggtcgacatg gcgcagtgcg ccaccgtgga cctgcaggtg ccggccgacg
    13081 ccgagatcgt cctcgagggc tacgtggaga agggcgagac gcggctcgag gggccgttcg
    13141 gcgaccacac cggctactac tccctggcgg atcagtatcc gctgttccac ctcacggcca
    13201 tgagccaccg ccgcgacccc atctacccgg ccaccatcgt cggcaagccg ccgatggagg
    13261 acgcctacct cggcaaggcg accgagcggc tgttcctgcc gctgctccag ctcgtgcagc
    13321 ccgagatcgt cgacatggac ctgccgatag agggcgtgtt ccacgactgc gccatcatca
    13381 gcatccgcaa ggagtacccg ggccacgcgc gcaagatcat gaacgccgtg tgggggatgg
    13441 ggcagatgat gttcaccaag ttcgtcgtcg tggtcgacga gcacgtggac gtgcacgact
    13501 acagcgaggt cacctggcgg gtgttcaaca acgtcgaccc cgagcgggac acggtcatcg
    13561 tccggggacc cctcgacgtg ctcgatcact ccagcccctt cgcccgctac ggcgccaaga
    13621 tgggcatcga cgccaccaag acctggccgg acgaggggca cgggcgcgag tggcccgagg
    13681 aactcgccat ggacccggcc gtggtcaggc gcgtcgacga gcgctggaag gagttcgggc
    13741 tgccgttctc ctgatccccc cggggtatcg gccccggggt tttgacccgt gtggcaggca
    13801 taagtgcagg atggtacaat cccggccaac tcgggcggcc tcgaagaggg gcaggccgcc
    13861 cgggatgaga tggagcccac ggggagtcac tcttcttgac cgaacgagaa cttcgccacc
    13921 gtcctgcgac ccgtccctgg tatcgccggg cgtggttcac ctggatcgtc gtcatcgcca
    13981 tcgcggtggt ggtcgtggcc atcctcgtgt tccccgtggc cgcgatggac gaccagtcct
    14041 actgcacgtc ctgcaaggcg atgcagccgg cggagaagac gctggccgcc tccgcccacg
    14101 ccggcatcga ctgcaccaag tgccacatcc cgcccggcgt cgtccccgcc gccaggtggc
    14161 gcctcgccga ggccaagaac gtgtgggccg actacctcgg catgccgagc accgccgaga
    14221 aggagcacgt ccccaccaac gccaactgcc tcgagtgtca cccgctcagc ggcatcccca
    14281 acgagtcaga cggcgtgcgc atgaaccacg aggagcacct caagctgcgc aacctgctgt
    14341 gcgtcgactg ccacgacacc gtctcccaca agctccccgg ccagaccgac ggcgtcagca
    14401 tggtcacgtg ctccatgtgc cacaacgaac agggcgcgcc cgacgcgtgc gattacagcc
    14461 acccggcgcc gccggcttcc gagcacgcgc cggacttcat gaaggagcac ggcaaggagg
    14521 cgctggtcga cgaagaagcc tgcctccgct gccatcacga caaaccagcg ttctgcgacg
    14581 actgccatgc gtacccgccg tccacccact actcaggaca gtggcgctac acccacggcg
    14641 aggacgcaga ggccgacccc gcgaactgcg aggcctgcca cgacgaggcc tactgctcgc
    14701 agtgccatca ggtcaaccac cccaacggct ggctccag
//
LOCUS       DASUMX010000155         5342 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_624532, whole genome shotgun sequence.
ACCESSION   DASUMX010000155 DASUMX010000000
VERSION     DASUMX010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 5342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5342
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_624532"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2495)
                     /gene="rpoB"
                     /locus_tag="VFZ86_07510"
     CDS             complement(<1..2495)
                     /gene="rpoB"
                     /locus_tag="VFZ86_07510"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEX5642172.1"
                     /translation="MNEPQAPRVRTSFAKLDQPLPLPNLIDIQKKSWDWFLSDGLSET
                     IADINPIKDYSEKLLVQFGEHQFGEPSKSLAECRNKDMTFSAPLTMKVAFINEETGEI
                     REQSVFMGDFPMMTDRGTFIIHGTERVVVTQLVRSPGAYIMEPKQAEREKQVLVANLM
                     PQRGSWLELEIDKKGTVNVRIDRKRKFPVSVLLRAMGYGSNDELIALFDDSVYIRNTL
                     EKDTTSSQEEALVELFRKQRPGEPPTLDGATAMLKGLFFDPKRYDLSRVGRHKLNVRL
                     HNHIKPADRPDADVRVLVNEDIIELIKRLISLPGKLGVPEDAKDYAAEANAFLATRRE
                     EIAHEIDEYEHFGNRRLRTVGELVQDAFRIGLARMERVIRERLTTEDPDTIVPATLVN
                     IRPVVAALKEFFGSSQLSQFMDQTNSLSGLTHRRRLSALGAGGLTRERAPIEVRDVHP
                     THYGRMCPIETPEGPNIGLIGSLASMATVDEFGFIRAPYRKVVKGKVTDEIVYLDASE
                     EEEEIVDPKTGEIRYVTIAQANAAFDGKTGKFEDELILARTRAGADIVQVPPADIDYM
                     DVSPTQLVSVATALIPFLEHDDANRALMGSNMQRQAVPLLHAEAPFVGTGMEFRAAVD
                     TGDVVVAERGGKVAYVDADHIEIDAGKGEVDTYDLVKFMRSNQGTLIHQKPIVFPGDK
                     VPAGAVLADGSSTDSGELALGRNLLVGFMSWEGFNFEDAIILSEKVVKDDLLSSIHIE
                     EYEVDARTTKLGDEEITRDIPNRSEESLKDLDERGIVRIGAEVNSGDLLVGKVTPKGE
                     TELTAEEKLIRAIFKEKAREVRDTSLKVPHGEGG"
     gene            complement(2733..3122)
                     /gene="rplL"
                     /locus_tag="VFZ86_07515"
     CDS             complement(2733..3122)
                     /gene="rplL"
                     /locus_tag="VFZ86_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018280846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEX5642173.1"
                     /translation="MADKTLTKDQLIEAIKGMTVLELSEMVKALEEEFGVSAAAPMAM
                     AAAPAAAEAAAPAEEQTQFDVVLAAFGDQKIQVIKVVRALTGLGLKEAKEVVESAPKA
                     IKEGVTKDEAEDAKKQLEDAGATVEIK"
     gene            complement(3184..3714)
                     /gene="rplJ"
                     /locus_tag="VFZ86_07520"
     CDS             complement(3184..3714)
                     /gene="rplJ"
                     /locus_tag="VFZ86_07520"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEX5642174.1"
                     /translation="MLRNDKDAVIAEVQQLLTDTENLFVSDYRGLTVAELAELRGKLR
                     ESGASFRIVKNTLGGIAADKAGREPVKDLLSGPTAVTFCGDDLVGAAKALSDFAKTHP
                     QLAVRGGLLDASLIDPDGVKALASLPPRDVLIAQVVGTMAAPMTGLVTVLQGTISGFV
                     RALDQVAQQRAAAGEA"
     gene            complement(3967..4674)
                     /gene="rplA"
                     /locus_tag="VFZ86_07525"
     CDS             complement(3967..4674)
                     /gene="rplA"
                     /locus_tag="VFZ86_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006302354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEX5642175.1"
                     /translation="MAKKGRTYTAAKRTIEPGRLYTPLEGVRLLKSLEGAKFDETVEV
                     HFRLGVDVRHADQIVRGTTVLPHGTGKEMRVAVFAEGDKAREAEEAGADIVGTDDLAK
                     QVQEGVFNFDIAIATPDMMGTVGKLGRILGPRGLMPNPKSGTVTFDVGKAVSDAKGGK
                     VEYRTDRSGIIHLSIGKKSFTEEMLVENYGAVLDEIVRAKPAAAKGKYIKSVNLAATM
                     SPSIELDPARTRDLFES"
     gene            complement(4762..5187)
                     /gene="rplK"
                     /locus_tag="VFZ86_07530"
     CDS             complement(4762..5187)
                     /gene="rplK"
                     /locus_tag="VFZ86_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012664221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEX5642176.1"
                     /translation="MAKKVLTKIKLQIPAGQASPAPPVGPALGQHGVNIMEFCKSFNA
                     QTQASGSTIVPVEITVYEDRTFTFITKTPPAAVLLREALGIDKGSGEPNRNKVGTVTR
                     DQVRAIAETKMPDLNANDVDAAMKIIEGTARSMGIEVEQ"
     gene            complement(5211..>5342)
                     /locus_tag="VFZ86_07535"
     CDS             complement(5211..>5342)
                     /locus_tag="VFZ86_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012692574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEX5642177.1"
                     /translation="PLSDFSGEVVEVNLDQSRLKVMVSIFGRETPVELAFDQVKKTS"
BASE COUNT          747 a         1701 c         1911 g          983 t
ORIGIN      
        1 ccgccctcgc cgtgcggcac cttgaggctg gtgtcgcgca cctcgcgcgc cttctccttg
       61 aagatggcgc ggatgagctt ctcctcggcg gtgagctcgg tctcgccctt gggggtgacc
      121 ttgccgacca ggaggtcgcc ggagttgacc tcggcgccga tgcgcacgat gccgcgctcg
      181 tcgaggtcct tgagcgactc ctccgagcgg ttggggatgt cgcgcgtgat ctcctcgtcg
      241 cccagcttgg tcgtgcgcgc gtcgacctcg tactcctcga tgtggatcga ggagagaagg
      301 tcgtccttga cgaccttctc actgaggatg atggcgtcct cgaagttgaa gccctcccag
      361 ctcatgaagc cgaccaggag gttgcggccg agcgcgagct cgccgctgtc ggtggaggac
      421 ccgtcggcga gaacggcgcc ggcgggcacc ttgtcgccgg ggaacacgat gggcttctgg
      481 tggatcagcg tgccctggtt ggagcgcatg aacttgacga ggtcgtaggt gtcgacctcg
      541 cccttgccgg cgtcgatctc gatgtggtcg gcgtcgacgt aggcgacctt gccgccgcgc
      601 tcggcgacga ccacgtcgcc ggtgtccacg gcggcccgga actccatgcc ggtgccgacg
      661 aacggcgcct cggcgtggag cagcggcacg gcctggcgct gcatgttgga gcccatgagg
      721 gcgcggttgg cgtcgtcgtg ctcgaggaac gggatgaggg ccgtggccac cgacaccagc
      781 tgggtggggc tcacgtccat gtagtcgatg tcggccggcg gcacctggac gatgtcggcg
      841 ccggcgcgcg tgcgcgccag gatgagctcg tcctcgaact tgccggtctt gccgtcgaag
      901 gccgcgttgg cctgggcgat ggtgacgtag cggatctcgc cggtcttggg gtcgacgatc
      961 tcctcttctt cctcggaggc gtccagatac acgatctcgt cggtgacctt gcccttgacg
     1021 accttgcggt acggagcgcg gatgaagccg aactcgtcca cggtggccat ggatgcgagc
     1081 gagccgatga gaccgatgtt cgggccttcc ggcgtctcga tggggcacat gcggccgtag
     1141 tgggtggggt gcacgtcgcg cacctcgatg ggcgcgcgct cgcgcgtgag gccgcccgcg
     1201 cccagggcgc tcaggcggcg gcggtgcgtg aggccggaca gcgagttggt ctggtccatg
     1261 aactgcgaga gctgcgagga gccgaagaac tccttgagcg cggcgacgac gggccggatg
     1321 ttgaccagcg tcgccggcac gatggtgtcc gggtcctcgg tggtgaggcg ctcgcggatc
     1381 acgcgctcca tgcgcgcgag gccgatgcgg aacgcgtcct gcaccagctc gcccacggtg
     1441 cgcaggcggc ggttgccgaa gtgctcgtac tcgtcgatct cgtgggcgat ctcttcgcgc
     1501 cgcgtggcga ggaaggcgtt ggcctcggcg gcgtagtcct tggcgtcttc cggcacgccg
     1561 agcttgcccg gcagggagat gagccgcttg atgagctcga tgatgtcttc gttcacgagc
     1621 acgcgcacgt ccgcgtccgg ccggtccgcc ggcttgatgt ggttgtgcag gcgcacgttg
     1681 agcttgtggc gccccacgcg cgagaggtcg tagcgcttgg ggtcgaagaa caggcccttg
     1741 agcatcgccg tggcgccgtc gagggtcggc ggttcgccgg ggcgctgctt gcggaagagc
     1801 tcgaccagcg cctcctcctg cgaggaggtc gtgtccttct cgagggtgtt gcggatgtac
     1861 accgagtcgt cgaagagggc gatcagctcg tcgttggagc cgtagcccat ggcgcgcagg
     1921 aggacgctca cggggaactt gcgcttccgg tcgatgcgca cgttcacggt gcccttcttg
     1981 tcgatctcga gctcgagcca cgagccgcgc tggggcatga ggttggcgac caggacctgc
     2041 ttctcgcgct ccgcctgctt gggctccatg atgtaggcgc ccggcgagcg caccagctgc
     2101 gtgacgacca cgcgctcggt gccgtggatg atgaaggtgc cgcggtcggt catcatgggg
     2161 aagtcgccca tgaacaccga ctgctcgcgg atctcgcccg tctcctcgtt gatgaaggcg
     2221 accttcatcg tgagaggggc cgagaaggtc atgtccttgt tgcggcactc ggccagcgac
     2281 ttggacggtt cgccgaactg atgctccccg aactgcacga gcagcttctc ggagtagtcc
     2341 ttgatcgggt tgatgtcggc gatggtctca ctcaagccgt cgctcaggaa ccaatcccac
     2401 gacttcttct ggatgtcgat gaggttgggg agcgggagcg gctgatcgag cttggcgaag
     2461 gacgtacgca cgcgaggcgc ctggggctcg ttcaagcgag tctcctctag ctaagcagtg
     2521 actgggaaaa agagcacacc acaccagagg aatcgaggta tggccgcagg taacgcacgg
     2581 ccaagtaatc taccacaacg aacaaggcga tgcaagacca cgcgccccac cgctgggcgg
     2641 gtcgccggct cgagcgacgg cggaaagacg gaggcccggg gcggcgctgc caccccgggc
     2701 ctccgggaca cgagaactcg ggaggagcgt gactacttga tctcgacggt ggcgccggcg
     2761 tcctcgagct gcttcttggc gtcctccgcc tcgtccttgg tgacgccttc cttgatcgcc
     2821 ttcggcgcgc tctcgacgac ctctttggcc tccttgaggc cgagaccggt cagcgcgcgc
     2881 acgaccttga tgacctggat cttctggtcg ccgaaggcgg cgagcacgac gtcgaactgc
     2941 gtctgctcct ctgccggggc ggcggcctcg gcggcggcgg gagccgcggc catggccatg
     3001 ggcgccgcag cgctgacgcc gaactcttcc tcgagcgcct tgaccatctc gctgagctcg
     3061 agcacggtca tgcccttgat ggcctcgatg agctggtctt tcgtgagagt cttgtctgcc
     3121 attgtcagtt cacctcgcgt ggggcttgaa agccttgctg gacgggatgg atgcggctgc
     3181 ggctcacgcc tcgccggcgg cggcgcgctg ctgcgccacc tggtcgaggg cacggacgaa
     3241 tccgctgatc gtgccctgaa ggacggtgac gagacccgtc atcggtgcgg ccatggtgcc
     3301 gaccacctgt gcgatgagca cgtcgcgcgg cgggaggctg gccagcgcct tgacgccgtc
     3361 cgggtcgatg agactcgcgt cgagcaggcc gccgcgcacc gcgagctgcg ggtgcgtctt
     3421 ggcgaagtcc gacagagcct tggcggcgcc gaccaggtcg tcgccgcaga aggtgaccgc
     3481 ggtaggcccg gacaggaggt ccttgaccgg ctcgcggccc gccttgtcgg cggcgatgcc
     3541 gcccagggtg ttcttgacga tcctgaagct cgcgccgctc tcgcgcagct ttccgcggag
     3601 ctcggcgagc tccgccaccg tgaggccgcg gtagtcgctg acgaacaggt tctccgtgtc
     3661 cgtgagcagc tgctgcacct cggcgatcac agcgtctttg tcgtttctca gcatctcgac
     3721 ctcgcagtcg caacaaaaaa cccgctcccg aagcggaagc gggcacgggc gcatggcctg
     3781 gactcgttct cgcctcgggt ggcggacccg tgtggatcca ttcagctccc gggggagcac
     3841 cgaccgtcat cggccgcgac gcacgtgcag cgccgcgagg cggcagtata gaacaacgaa
     3901 ggggcgccgg caactgccgc ggccggtgcg gtgcacctgc ggcggccgcc ggcgccccct
     3961 ttgtgcttag ctctcgaaga gatcccgcgt gcgcgccggg tcgagctcga tgctcgggct
     4021 catcgtcgcg gcgagattga cgctcttgat gtacttgccc ttggcggccg ccggcttcgc
     4081 gcggacgatc tcgtccagca ccgcgccgta gttctccacc agcatctcct cggtgaacga
     4141 cttcttgccg atgctgaggt ggatgatgcc ggagcggtcg gtgcggtact cgaccttgcc
     4201 gcccttggcg tcgctgacgg ccttgccgac gtcgaacgtc acggtgccgg acttggggtt
     4261 gggcatgagg ccgcgcggac cgaggatgcg gccgagcttg ccgacagtgc ccatcatgtc
     4321 cggggtcgcg atggcgatgt cgaagttgaa gacgccctcc tggacctgct tggcgaggtc
     4381 gtcggtgccg acgatgtcgg cgccggcctc ctcggcctca cgcgccttgt cgccctcggc
     4441 gaacacggcg acgcgcatct ccttgccggt gccgtgcggg agcaccgtgg tgccgcgcac
     4501 gatctggtcg gcgtggcgga cgtcgacccc gaggcggaag tggacctcga cggtctcgtc
     4561 gaacttggcg ccctcgagtg acttgagcag gcgcacaccc tcgagcggag tgtagaggcg
     4621 tccgggctcg atggtgcgct tggcggcggt gtaggtgcgg cccttcttgg ccatgtgcta
     4681 cctcctgtgg tgcaagcggc gacactggtc gcctcccacg tcggcggccg gtcggcggga
     4741 gccgggccgg ccttgttcgt cctactgttc gacctcgatg cccatgctgc gggcggtgcc
     4801 ctcgatgatc ttcatcgcgg cgtcgacgtc gttggcgttg aggtcgggca tcttggtctc
     4861 ggcgatggcg cggacctggt cgcgggtgac cgtgccgacc ttgttacggt tgggctcgcc
     4921 ggagcccttg tcgatgccga gggcctcgcg cagaagcacg gcggccggcg gcgtcttggt
     4981 gatgaaggtg aacgtgcggt cctcgtacac ggtgatctcg accggcacga tggtgctccc
     5041 ggacgcctgg gtctgggcgt tgaacgactt gcagaactcc atgatgttga cgccgtgctg
     5101 gccgagcgcc gggcccacgg gcggggcagg gctcgcctga ccggcgggga tctgcagctt
     5161 gatcttggtg aggaccttct tggccactgg ttgcctcgct tacttcctgg ttaggacgtc
     5221 ttcttgacct ggtcgaaggc gagctcgacc ggcgtctcgc ggccgaagat cgacaccatg
     5281 accttgagcc ggctctggtc gaggttgacc tcgacgacct cgccggagaa atcgctgagc
     5341 gg
//
LOCUS       DASUMX010000156        13082 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1629502, whole genome shotgun sequence.
ACCESSION   DASUMX010000156 DASUMX010000000
VERSION     DASUMX010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 13082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13082
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1629502"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="VFZ86_07540"
     CDS             <1..285
                     /locus_tag="VFZ86_07540"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5642178.1"
                     /translation="SKGAVDALTRSLAAQWAEHGIRVNAVAPTFVETDLTRSVLADAQ
                     FAAGLRASIPMGRWGETEDVVGPVLFLASTAARFVTGQILYVDGGLTARV"
     gene            565..1365
                     /locus_tag="VFZ86_07545"
     CDS             565..1365
                     /locus_tag="VFZ86_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5642179.1"
                     /translation="MADREIWGVDKIRNLFDLSGKTAIVTGGSGAFGHDAAKGLAAFG
                     ANVVVTSRRAETLEAAAKDIAGATGARVRAVSCDAVDPESVEAMVAATVAEFGAVDIL
                     VTAAGQADRHPAEEFPIDAWQMVMDSLVKGTYLCCQSAGRQMIAQGGGKIITVGSVRG
                     EFGHPGGYSAYGTAKGAVHLLTKQLSTEWAKHHINVNSIAPCIFWTPLTQQVIEDPEL
                     KKIFLSRIPWGRVADPQDFIGAVVFLASAASDFVTGEIMAVDGGSTAG"
     gene            1391..2335
                     /locus_tag="VFZ86_07550"
     CDS             1391..2335
                     /locus_tag="VFZ86_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005037772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEX5642180.1"
                     /translation="MESAERVAVIGGGMMGVGVAQVFAAAGHEVALQDVHPEALERAP
                     GTLTANLSLLAQHHLFPADGVEDAVGRVRTTTDLSDACEGADVVVECVFEDLALKQDV
                     FERLDAACRPETILCTNTSVMSITQIGEKTRHKERVVGTHFWNPPYLIPLVEVVRTEQ
                     VDPAVVDATMGLLRRVGKRPIDCRKDVPGFVANRLQHALWREAISIVEHGIADAATVD
                     ESIRFGPGLRLPILAPMENADMVGLDLTLAIHDYILPHLEASGEPSPLLRDKVAAGDL
                     GFKSGQGFFAWSPEAADASCKRLAAYLVHVLGDAERGF"
     gene            2367..3119
                     /locus_tag="VFZ86_07555"
     CDS             2367..3119
                     /locus_tag="VFZ86_07555"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX5642181.1"
                     /translation="MTERRDGVQVLSRAALLLRELSVAPEGLTPIALADRVGLPRSTC
                     YRIAGALCQEGFMRLAPSGKLHIGAGLISIAAAGRRELRHEAAPFLRRLSLELHETVE
                     LVVLDGDEALFTDQYVPQRSLRVVAEVGDRFPLYCTACGKAILAELSEEEAERLIPDR
                     IRPLTGHTIVDRRALLREIEAARAAGVAFDRQEHTLGVSAVGAAVQDPGGSMAAITVA
                     MPAARLEGQEERIVAALLRARNDIQKAIAHAG"
     gene            3259..4218
                     /locus_tag="VFZ86_07560"
     CDS             3259..4218
                     /locus_tag="VFZ86_07560"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007491232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX5642182.1"
                     /translation="MGKKVFVDLTHPFSAEIPRWPYFVKPVIDSMHTLAKGGVLTQRI
                     DCVQHTGTHADAPRHVMEREFNGKRARYTHEMPVDAYTGDAVCLDIQIEKWGLILPSH
                     LEDACARAGIKPEELKGMVVCLNTGMHRKFDDSMDYYQYSCGTGVEAGRWFVEKQVKV
                     VAMDMQALDHPLHTAMGKNGPPQMNLPGASGRPITEEYIEKYGIEAYAEFEKAVYIEV
                     HGQDAYDAKFGDLEAIGCEGTWEPCHKQMLGHGIVGVENLGGDLDKVKGKRFRFFCFP
                     IRWYMGDGSMVRCAAEIDEDDLNDVPDRTYTYAGTGYGPGAGY"
     gene            4251..4601
                     /locus_tag="VFZ86_07565"
     CDS             4251..4601
                     /locus_tag="VFZ86_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007986468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5642183.1"
                     /translation="MEVTRYDEAPSYEAPGHFDVYGLRLQGFEVSKTRNFWMSISHFL
                     PKGGCTYDATPLEKVYFVLDGEVTIKTEEGEVVLKKWDSIYLAPNETRSIENRTNSPA
                     SMLVAMPYPEPREF"
     gene            4735..5565
                     /locus_tag="VFZ86_07570"
     CDS             4735..5565
                     /locus_tag="VFZ86_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003902211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HEX5642184.1"
                     /translation="MPNPIITAALTGPVATKADNAGMPGSVAEIAADAKACHEAGAAV
                     IHIHLRDDQGSMTADLDVARRTVAAVREVCPGITQLSTGGLQFTYEQRMALVEARPAM
                     ATLNPCTMTFGAAEFKNPPKQMMELAARMIELGVKPEVEIYDTGHLDMMLYLLHKELL
                     VEPLQVSFVMGVRGGMRGDPQLLSWLVRELPEGTSWQVIAVAKANLPMTTIGLAMGGN
                     ARTGMEDTLTLSKGVPAASNLQLVERLVGIAHSMQLEPATVPEVIERLKLHPELVSGA
                     "
     gene            5857..7230
                     /locus_tag="VFZ86_07575"
     CDS             5857..7230
                     /locus_tag="VFZ86_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019860236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5642185.1"
                     /translation="MSSEDKTTKGTESVIRTPLSRRDFLKTAGVAGLALGATGGLGAV
                     AAACGGGETSDSASEGEGREIKIGFVSPLTGPLAAFGEADQFCVDQWNAAVKDGIATA
                     DGAVHPINIIIKDSQSDSNRAATVAGDLINNDGVDIMMVASTPDTVPPVADQCEAFGV
                     PCVSNDCPWQAYFFGRGGDPENPFKWTYHSFWGSESAAAVFMAMWDSFDTNKKIGVMW
                     PNDADGNAWADPETGMPSYWEPAGYTFVDPGRYQNGTEDFTSQISQFKAAGVDILNGV
                     MIPPDFTNFWKQAYQQGLKPPFVTIAKALLFPSALEALGDIGNGLTTEVWWTPQHPYK
                     SSLTDETSQQIGDAYESKTGKQWTQPIMHYAVFEVVADAIKRTENIDDKESIIAAVEA
                     TNIETISGPVSWSGGENNPVKNVSITPLVGGQWVPGEKYMYDLKIVSNTIAPDVPTNG
                     ELKLMEY"
     gene            7311..8036
                     /locus_tag="VFZ86_07580"
     CDS             7311..8036
                     /locus_tag="VFZ86_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018303532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5642186.1"
                     /translation="MTVVLQTSGLSKSFGGVKVIDDLSVQLSEGEALGIVGPNGAGKT
                     TFFNLVSGVYRPDSGRVEFKGKDVTHTARQDICRLGLGRTYQIPKPFSDMSVFENVLV
                     CAAYARGLSERASYERCVEVLEQTRLSHKANHRAGALPLLDRKRLELARALAGDPSVL
                     LLDEIAGGLTESEVEILIGTIKDLRLGGISIIWIEHIVHALLAVVDRICAISFGALLI
                     EGDPNEVMCSDEVQACYMGGTPL"
     gene            8039..8731
                     /locus_tag="VFZ86_07585"
     CDS             8039..8731
                     /locus_tag="VFZ86_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007997988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5642187.1"
                     /translation="MLELKDVDVFYGDFQALFSVSFTVQEGQTFAFIGANGGGKSTLL
                     RTLAGLLKPHSGEVLFQGEPINDVPAHSRVGMGIALVPEGRRVFPSLTVNENLLIGAY
                     GGRKGEWNVESVLELFPLLQPLMKRQASSLSGGEQQALAIGRALMSNPHLLLLDEVSL
                     GLAPVVIKRIYEAMGHLVQKGTTILVVEQDVQHVLTVADHVACFLEGRISLEGKPGDL
                     SSEQITAAYFGM"
     gene            8736..9608
                     /locus_tag="VFZ86_07590"
     CDS             8736..9608
                     /locus_tag="VFZ86_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003554581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX5642188.1"
                     /translation="MDWVNVALQGVLLGGLFALFATGLALVFGVMRLVNLAHGDLGIL
                     AAFVALFLLDSFDIGVFWASLAAVVIMFGIGYVLQRGLLNFTIGTDDTRPILVTFGLS
                     IIIQNGLVMWFSADSQGLDAGRLENASVRLTDQLAVGWYPLIVFVIAVVVIAALQLFL
                     NRTTLGRAFRATSDDQEAAELMGINNRHIYGLAMAIALAIVALAGILLAVRTTFDPSQ
                     GPFRLIFAFEAVIMGGMGSIWGTLIGGIVLGVSQTLGAQAFGAGWGIFVGHVVFLTVL
                     AFKPSGFFAKTVTA"
     gene            9605..10693
                     /locus_tag="VFZ86_07595"
     CDS             9605..10693
                     /locus_tag="VFZ86_07595"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX5642189.1"
                     /translation="MTPPAETPQATAVPAFRVARSARMHRYVLVAFVAVVVALATVPL
                     WAETATTRTLVTFFTLLALAQMWNLLGGYAGLISVGQQAYVGIGAYGVWLIGDVLGIH
                     PFVSVLLAGVLAALIALPIGPLIFRLKGGYFAIGTWVVAEVVRLVVANIQATGGGSGK
                     TIMSAARLPMETRVPGTYYLALAVAVGSILLVYFLLRSRVGLALTAIRDNDLAAQSSG
                     VNVFRAKLLVYVVAAFGCGAMGAVVAMNLLRIQPTAAFSINWTAYAIFIVVIGGFGTI
                     EGPLIGSVIYFALLQTLSQYGTSYMLLLGVIAVIMAAKAPRGIWGFVEKRWDIHLFPV
                     RRELLLESPPPEERKPADRPDAAATETA"
     gene            10868..11647
                     /locus_tag="VFZ86_07600"
     CDS             10868..11647
                     /locus_tag="VFZ86_07600"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX5642190.1"
                     /translation="MIETDRDEEREQRNGVQVLARAATILRLLAADSSGGLTFSELVA
                     RSGLPRTTVHRIRGALEHEDFIYTDAATGRLHLGPGIMRLAMAKRDLPTVVRPYLERL
                     SRELNETVDLGVLDGMHVLFIAQHPAPQRSLMVVSRVGARFPAYCTANGKALLAQLPE
                     DEIKRRLPKKLETPARHTPVSRKALLAELDDVRATGLGFDREEHRAGICGVGVAITDI
                     DGSSASISVPMPAARFHEDPDTVAAALLRVRDEVQIALHNS"
     gene            11675..12244
                     /locus_tag="VFZ86_07605"
     CDS             11675..12244
                     /locus_tag="VFZ86_07605"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HEX5642191.1"
                     /translation="MDARPSVVAVIASPRRGNTAALVGAAVAELEARGAACETIALGD
                     LFIAPCQAHDGCGDFAACPLRDDAAAVLDRVYAADCLILGSPVYYENVSAQMKAFIDR
                     NVFRYAHDEWLRATAVGLVAVTAETGLQETLDALRRYVALSTDHDVPVFTCGGYADAA
                     GAAAADEALLAEARRLGAELASALGLSRA"
     gene            complement(12231..12839)
                     /locus_tag="VFZ86_07610"
     CDS             complement(12231..12839)
                     /locus_tag="VFZ86_07610"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEX5642192.1"
                     /translation="MSQPRDQSRGDDRALPKAEARRRALAARDALPEDARERLAAAVR
                     ARALELPELAAAGTVMLFASFRSELDTAPIAEWVLRAGKTLCLPRILGPRRMASFRVT
                     DRAADLAPGTWGIPEPREGLPEVPPQTIDLVFVPGSAFDEEGGRCGWGGGFYDTYLPL
                     TRPGTPRVALAFEAQLVPRIAGEAHDLPVTAIVTERRVIRPG"
BASE COUNT         2006 a         4625 c         4508 g         1943 t
ORIGIN      
        1 agcaagggcg ccgtggacgc gctcacgcgg tcgctcgccg cccagtgggc ggagcacggc
       61 atccgcgtca acgccgtggc gccgaccttc gtcgagaccg acctcacgcg ctcggtcctg
      121 gccgacgcgc agttcgccgc cgggctgcgc gccagcatcc cgatgggtcg ctggggcgag
      181 accgaagacg tcgttggccc ggtgctgttc ctcgcctcga cggcggcgcg gttcgtgacc
      241 ggccagatcc tgtacgtgga cggcggtctc acggcgcgcg tctagcttct ccgcgcagcg
      301 gaactctcgg tccggtacgc agccgatcgg cgaccgcggc ggccttcgcg cacgcacggc
      361 gccgatgctt gacctgcccg aacccctgca ctagtgttcg catcatggac tagttgttcc
      421 gcatcgtggg cactcgcccg cgacagggac aggcgtgagg gggcccggtg gagggtcggt
      481 cccgcggcgg cagggcgtcc gatcccgccg ccggaccggc gcacgaccgg accgggccgg
      541 acacgaggtg agaggagaac tgcgatggct gaccgtgaga tctggggcgt cgacaagatc
      601 cgcaatctgt tcgacctgag cggcaagacg gcgatcgtca ccggcggctc cggcgcgttc
      661 ggccacgacg ccgccaaggg cctcgcggcc ttcggcgcca acgtcgtggt caccagccgt
      721 cgcgccgaga ccctcgaggc ggcggccaag gacatcgccg gcgccaccgg cgccagggtc
      781 agggccgtgt cttgcgacgc cgtcgatccg gagagcgtcg aggccatggt cgctgcgacc
      841 gtcgccgagt tcggtgcggt cgacatcctc gtcaccgccg caggtcaggc cgaccgccac
      901 cccgccgagg agttccccat cgacgcctgg cagatggtca tggactcgct cgtcaagggc
      961 acctacctct gctgccagtc cgccggccgc cagatgatcg cgcagggcgg cggcaagatc
     1021 atcaccgtcg gctccgtgcg cggcgagttc ggccacccgg gcggctactc cgcctacggc
     1081 accgccaagg gcgcggtgca cctgctcacc aagcagctct ccaccgagtg ggccaagcac
     1141 cacatcaacg tgaacagcat cgccccgtgc atcttctgga ccccgctcac gcagcaggtc
     1201 atcgaggatc cggagctcaa gaagatcttc ctctcgcgca tcccgtgggg ccgcgtcgcc
     1261 gatcctcagg acttcatcgg cgccgtcgtc ttcctcgcct ccgccgcctc cgacttcgtc
     1321 accggcgaga tcatggccgt ggacggcggc agcacggccg gctgagcgcc ggccggcgag
     1381 gaggtcgatc atggagagcg ccgagcgcgt cgccgtcatc ggcggcggca tgatgggcgt
     1441 gggcgtcgcc caggtcttcg ccgccgccgg gcacgaggtc gcgctccagg acgtccatcc
     1501 cgaggccctg gagcgcgccc ccggcacgct cacggccaac ctgtccctcc tggcccagca
     1561 ccacctgttc ccggccgacg gcgtcgagga cgccgtcggc cgggtccgca ccaccaccga
     1621 cctgtccgac gcctgcgagg gcgcggacgt ggtcgtggag tgcgtgttcg aggacctcgc
     1681 gctcaagcag gacgtcttcg agcggctgga cgccgcctgc cggccggaga cgatcctctg
     1741 caccaacacg tcggtcatga gcatcacgca gatcggcgag aagacgcggc acaaggagcg
     1801 cgtggtgggc acgcacttct ggaacccgcc gtacctgatc ccgctcgtgg aggtggtccg
     1861 gacggagcag gtcgacccgg ccgtggtgga cgcgaccatg ggcctgctgc ggcgcgtcgg
     1921 caagcggccc atcgactgca ggaaggatgt acccgggttc gtcgccaacc gtctgcagca
     1981 cgcgctgtgg cgcgaggcca tctccatcgt cgagcacggc atcgccgacg ccgccacggt
     2041 ggacgagagc atccgtttcg gccccggcct gcggctgccg atcctcgccc ccatggagaa
     2101 cgccgacatg gtggggctcg acctcacgct ggcgatccac gactacatcc tgcctcacct
     2161 cgaggcgtcg ggtgagccgt cgccgctgct gcgcgacaag gtcgccgccg gcgatctggg
     2221 gttcaagtcg gggcaggggt tcttcgcgtg gtcgccggag gcggcggacg cctcgtgcaa
     2281 gcgcctggct gcgtacctcg tgcacgtgct gggcgacgca gagcggggct tctgaccgcc
     2341 ggccgggtcg gaggccggga ggcgagatga ctgagcggcg cgacggtgtg caggtgctct
     2401 cgcgcgcggc gctgctgctg cgcgagctct cggtcgcgcc cgagggcctc acgcccatcg
     2461 ctctggccga ccgcgtgggc ctgccgcgct cgacctgcta ccgcatcgcc ggggcgctgt
     2521 gccaggaggg gttcatgcgc ctggcgccct ccggcaagct gcacatcggc gccggcctca
     2581 tcagcatcgc cgccgcggga cgccgcgagc tgcgccacga ggcggcgccc ttcctcaggc
     2641 ggctctcgct cgagctgcac gagacggtgg agctcgtcgt gctcgacggt gacgaggcgc
     2701 tcttcaccga ccagtacgtc ccgcaacgct cgctgcgcgt ggtcgcggag gtcggggaca
     2761 ggttcccgct ctactgcacg gcgtgcggca aggcgatcct cgctgagctg tccgaggaag
     2821 aggccgagcg gctcatcccg gatcgcatcc ggccgctcac cggccacacc atcgtcgacc
     2881 gccgcgcgct gctgcgggag atagaggcgg cgcgcgctgc cggtgtcgcc ttcgaccgcc
     2941 aggagcacac gctcggcgtc tcggcggtcg gcgccgccgt ccaggatccg ggcgggtcga
     3001 tggccgccat cacggtggcc atgccggccg cccgtctcga gggccaggag gagcgcatcg
     3061 tcgccgcgct cctgcgcgcg cgcaacgaca tccagaaggc catcgcgcac gccggctgag
     3121 gcgcggcccc gcccggcaga cagagccggc cctcgtgcaa cggcggcgta ttgacagcgg
     3181 attccgaaga ccggtaccgt atccacgata cggcatcaag tgtccgccat gcggacagta
     3241 gcgaggagaa gcagacctat gggcaagaag gtgttcgttg acctcacaca tccgttcagc
     3301 gcggagatcc cccggtggcc gtacttcgtg aagccggtca tcgactcgat gcacaccctg
     3361 gccaagggcg gcgtcctcac gcagcgcatc gactgcgtgc agcacaccgg cacccacgcc
     3421 gacgctcctc gtcacgtcat ggagcgtgag ttcaacggca agcgtgcccg ctacacgcac
     3481 gagatgccgg tcgacgccta caccggcgac gccgtctgcc tcgacatcca gatcgagaag
     3541 tggggcctca tcctgcccag ccacctcgag gacgcctgcg cccgcgccgg catcaagccg
     3601 gaggagctca agggcatggt cgtgtgcctc aacaccggca tgcaccgcaa gttcgacgac
     3661 tccatggact actaccagta ctcgtgcggc acgggcgtcg aggccggcag gtggttcgtc
     3721 gagaagcagg tcaaggtcgt ggccatggac atgcaggccc tcgaccatcc gctccacacc
     3781 gcgatgggca agaacggccc gccgcagatg aacctcccgg gagcctccgg ccgtccgatc
     3841 accgaggagt acatcgagaa gtacggcatc gaggcctacg ccgagttcga gaaggccgtc
     3901 tacatcgagg tgcacggcca ggacgcctac gacgccaagt tcggcgacct cgaggcgatc
     3961 ggctgcgagg gaacgtggga gccctgccac aagcagatgc tcggtcacgg catcgtcggc
     4021 gtcgagaacc tcggcggcga cctggacaag gtcaagggca agcggttccg cttcttctgc
     4081 ttcccgatcc gttggtacat gggcgacggc tccatggtcc gctgcgccgc ggagatcgac
     4141 gaggacgacc tgaacgacgt gccggaccgc acgtacacct acgccggcac cggctacggc
     4201 cccggagccg gctactgaga agccgggaac ggctcgagga ggagtgaggc atggaggtaa
     4261 cgaggtacga cgaggcgccg agctacgagg ctcccggtca tttcgacgtg tacggcctgc
     4321 gcctgcaggg cttcgaggtc agcaagaccc gtaacttctg gatgagcatc tcccacttcc
     4381 tgcccaaggg cgggtgcacg tacgacgcca ctccgctcga gaaggtgtac ttcgtgctcg
     4441 acggcgaggt gaccatcaag acggaagagg gcgaggtcgt gctgaagaag tgggactcga
     4501 tctacctcgc gccgaacgag acgcgctcga ttgagaaccg caccaactcg ccggcgtcga
     4561 tgctcgtcgc catgccgtac ccggagcccc gcgagttctg aggcagccgc cgggaccttc
     4621 ccggcccccg gcggggaaga cgcgcacagg ccaacggccg gcgcggggac gcgcggcggc
     4681 aggaccgccg cgcgtccccg cgcgctgacc acacgacctc cgaggagggt ctcaatgccc
     4741 aacccgatca tcaccgcggc gctcacgggg cccgtggcga ccaaggcgga caacgccggc
     4801 atgccgggca gcgtcgcgga gatcgccgcc gacgccaagg cgtgccacga agccggcgcg
     4861 gcggtcatcc acatccacct gcgcgacgac cagggcagca tgacggccga tctcgacgtg
     4921 gccaggcgga cggtcgcggc cgtgcgcgag gtgtgccccg gcatcacgca gctgtccacc
     4981 ggcggcctgc agttcacgta cgagcagcgc atggcgctgg tcgaggcgag gcccgcgatg
     5041 gcgaccttga acccgtgcac catgaccttc ggcgcggcgg agttcaagaa cccgcccaag
     5101 cagatgatgg agctcgcggc gcggatgatc gagctgggcg tcaagcctga ggtcgagatc
     5161 tacgacaccg gtcacctcga catgatgctc tacctgctcc acaaggagct cctcgtcgag
     5221 ccgctccagg tgtcgttcgt gatgggcgtc cgcggcggca tgcgcggcga cccccagctg
     5281 ctctcgtggc tcgtgcgcga gctgccggag ggcaccagct ggcaggtcat cgccgtcgcc
     5341 aaagccaacc tgccgatgac gaccatcggc ttggccatgg gcgggaacgc gcgcacgggg
     5401 atggaggaca cgctgacgct ctccaagggc gtgccggcgg cgtccaacct gcagctggtc
     5461 gagcgcctcg tcggcatcgc ccactccatg cagctcgagc cggcgaccgt gccggaggtg
     5521 atcgagcgtc tgaagctgca cccggagctc gtcagcgggg cctgattgct tgtgccgggc
     5581 ggcacaaaca ccgctccgga cgcagtcgtg gcgtgcttga caccgtccgg cccggctata
     5641 tgatgcccgt atcgtgggag gaagcgtccg tatggtggat agccatggag gtggtgcgca
     5701 ccgtacaagc tgactcaccg ggaacccacg gctgcgggag cagcagcacg aacagccgtt
     5761 cgggggatcc gggaccagaa ggccatcggg ggggtcccgg ctgccggcgt cgctcatggc
     5821 gggtggcgag gcggcgggac tacgtaagga ggcggtatga gttctgagga caagaccacc
     5881 aagggcacgg agtctgtgat ccgcacgccg ctctcgcggc gcgacttcct caagacggcc
     5941 ggcgtcgccg gactcgcgct cggcgcgacc ggcgggctcg gcgccgtcgc ggcggcctgc
     6001 ggtggcgggg agaccagcga cagcgccagc gagggcgagg gccgcgagat caagatcggc
     6061 ttcgtctcgc cgctcaccgg gccgctcgct gccttcggcg aggcggacca gttctgcgtc
     6121 gaccagtgga acgccgccgt caaggacggt atcgccacgg ccgacggggc ggtccacccg
     6181 atcaacatca tcatcaagga ctcgcagtca gactcgaacc gcgccgcgac ggtggcgggc
     6241 gacctcatca acaacgacgg cgtggacatc atgatggtcg cctccacgcc ggacacggtg
     6301 ccgccggtgg cggaccagtg cgaggccttc ggcgtcccct gcgtgtccaa cgactgcccg
     6361 tggcaggcct acttcttcgg ccgcggcggc gaccccgaga accccttcaa gtggacctac
     6421 cactcgttct ggggctccga gtcggcggcg gccgtgttca tggccatgtg ggacagcttc
     6481 gacaccaaca agaagatcgg cgtcatgtgg ccgaacgacg ccgacggcaa cgcctgggcc
     6541 gacccggaga cgggcatgcc cagctactgg gagccggcag gctacacgtt cgtggatccc
     6601 ggccggtacc agaacggcac cgaggatttc acctcccaga tctcccagtt caaggcggcc
     6661 ggagtcgata tcctcaacgg cgtcatgatc ccgccggact tcacgaactt ctggaagcag
     6721 gcgtaccagc agggcctcaa gcccccgttc gtcacgatcg ccaaggctct gctcttcccg
     6781 tcggccctcg aggccctcgg cgacatcggc aacgggctca ccaccgaggt gtggtggacg
     6841 ccgcagcacc cgtacaagtc gtcgctcacc gacgagacca gccagcagat cggcgacgcg
     6901 tacgagtcca agaccggcaa gcagtggacc cagccgatca tgcactacgc ggtcttcgag
     6961 gtcgtggcgg acgccatcaa gcgcaccgag aacatcgacg acaaggaatc catcatcgcc
     7021 gcggtcgagg ccacgaacat cgagaccatc agcggacctg tgtcctggag cggcggcgag
     7081 aacaaccccg tcaagaacgt gagcatcacg ccgctggtcg gcggccagtg ggtgcccggc
     7141 gagaagtaca tgtacgacct caagatcgtc agcaacacga tcgcgccgga cgtccccacc
     7201 aacggcgagc tcaagctcat ggagtactga gtgactgacc cgatccacgt gcccggtcac
     7261 ccgtctccgc atcattcgga ggtagcagcg atgcagggtg agagaccctc atgaccgtgg
     7321 tgctccagac cagcggtctc agcaagtcct tcggcggcgt gaaggtcatc gacgacctga
     7381 gcgtgcagct gagcgagggc gaggcgctgg gcatcgtcgg gccgaacggc gccggcaaga
     7441 cgaccttctt caacctcgtc tccggcgtgt atcggcccga ctcgggccgg gtcgagttca
     7501 agggcaagga cgtcacgcac acggcgcggc aggacatctg ccgcctgggc ctgggccgca
     7561 cctaccagat ccccaagccg ttctcggaca tgagcgtctt cgagaacgtg ctcgtgtgcg
     7621 ccgcgtacgc ccgcggcttg agcgaacgcg cgagctacga gcgctgcgtc gaagtgctcg
     7681 agcagacgcg gctctcgcac aaggcgaatc accgggcggg cgccctgccc ctcctcgacc
     7741 gcaagcggct cgagctggcc agggcgctcg ccggcgatcc cagcgtgctg ctgctggacg
     7801 agatcgccgg cggcctgacg gagagcgaag tggagatcct catcggtacc atcaaggacc
     7861 ttcgcctggg aggcatctcg atcatctgga tcgagcacat cgtccacgcg ctgctcgcgg
     7921 tcgtcgaccg catctgcgcg atcagcttcg gcgccctgct catcgagggc gaccccaacg
     7981 aggtcatgtg cagcgacgag gtgcaggcct gctacatggg ggggacgccg ctgtgagcat
     8041 cctcgagctc aaggacgtcg atgtcttcta cggcgatttc caggctctct tctcggtcag
     8101 cttcaccgtc caggaggggc agacgttcgc gttcatcggc gccaacggcg gcggcaagtc
     8161 gaccctgctc cggacgctcg ccggcctgct caagccgcac tccggcgagg tgctgttcca
     8221 gggcgagccg atcaacgatg tcccggcgca ctcgcgcgtc ggtatgggca tcgccctcgt
     8281 gcccgaggga cgccgcgtgt tcccgagtct caccgtcaac gagaacctgc tcatcggcgc
     8341 ctacggaggg cgcaagggcg agtggaacgt cgagtccgtg ctcgagctct tcccgcttct
     8401 ccagcccctc atgaagcgcc aggcctccag cctgtcgggc ggcgagcagc aggcgctcgc
     8461 catcgggcgc gcgctgatgt cgaacccgca cctcctcctg ctggacgagg tctcgctggg
     8521 tctcgcgccc gtcgtcatca agcgcatcta cgaggcgatg ggtcatctgg tgcagaaggg
     8581 cacgaccatc ctcgtcgtcg agcaggacgt gcagcacgtc ctcacggtgg cggaccacgt
     8641 cgcctgcttc ctcgagggca ggatctccct tgagggaaag cccggcgacc tgagttccga
     8701 gcagataacc gcggcctact tcgggatgtg atcgtatgga ttgggtgaac gtcgccctgc
     8761 agggcgtgct cctgggtggg ctcttcgcac tgttcgcgac cgggctcgcc ctcgtcttcg
     8821 gcgtgatgcg gctggtcaac ctcgcgcacg gcgacctcgg catcctcgct gcgttcgtcg
     8881 ccctgttcct gctggacagc ttcgacatcg gcgtgttctg ggcgtccctc gcggccgtcg
     8941 tcatcatgtt cggcatcggc tacgtgctgc agcgcgggtt gctcaacttc accatcggca
     9001 ccgacgacac gcggccgatc ctggtcacgt tcggcttgtc gatcatcatc cagaacgggc
     9061 tggtgatgtg gttctcggcc gactcacagg gtcttgacgc cggccgcctc gagaacgcca
     9121 gcgtgcggct gacggaccag ctcgcggtcg gctggtaccc gctgatcgtg ttcgtcatcg
     9181 ctgtcgtggt catcgcggcg ctgcagctct tcctcaaccg gacgaccttg ggccgcgcct
     9241 tccgggccac ctcggacgat caggaagcgg cggagctgat gggcatcaac aaccgccaca
     9301 tctacggtct ggccatggcc atcgctttgg cgatcgtcgc gctcgccggc atcctgctcg
     9361 cggtccgcac cacgttcgac ccgagccagg gcccgttccg cctcatcttc gccttcgagg
     9421 cggtcatcat gggcggcatg ggctccatct gggggacgct catcggaggg atcgtgctcg
     9481 gcgtctcgca gacgctcggg gcgcaggcct tcggcgccgg ctggggtatc ttcgtcggcc
     9541 acgtcgtgtt cctgaccgtg ctcgccttca agcccagcgg cttcttcgcg aagacggtga
     9601 cggcatgact cctcccgccg agacccctca ggcgacggcc gtcccggcgt tccgcgtcgc
     9661 gcgttcggcg cggatgcacc gttacgtgct cgtcgccttc gtggccgtcg tcgtcgcgct
     9721 ggccaccgtc cccctctggg cggagaccgc caccacccgt accctggtga cgttcttcac
     9781 gctgctggcc ctcgcccaga tgtggaacct gctcggcggc tacgccggcc tcatcagtgt
     9841 gggccagcag gcctacgtgg gcatcggcgc ctacggcgtc tggctcatcg gcgacgtcct
     9901 gggcatccac cccttcgtct cggtgctcct ggccggcgtg ctggcggccc tcatcgctct
     9961 gccgatcggg ccgctgatct tccgcctcaa gggcggctac ttcgccatcg gcacgtgggt
    10021 cgtggcggag gtcgtccggc tggtcgtggc caacatccag gcgacgggcg gcggctccgg
    10081 caagaccatc atgtcggccg cccgcctccc gatggagacg cgcgtcccgg gcacgtacta
    10141 cctggcgctc gccgtcgccg tcggctccat cctgctcgtg tacttcctcc tgcgctcgcg
    10201 cgtgggcctg gccctcaccg ccatccgtga caacgacctg gcggcgcaga gctcgggcgt
    10261 caacgtgttc cgcgccaagc tgctcgtgta cgtcgtggcc gccttcggct gcggggccat
    10321 gggcgccgtc gtcgccatga acctgctgcg catccagccg acggccgcgt tcagcatcaa
    10381 ctggacggcg tacgcgatct tcatcgtggt gatcggcggc ttcggcacca tcgaggggcc
    10441 gctgatcggc tccgtcatct acttcgcgct cctgcagacg ctcagccagt acggcacgtc
    10501 gtacatgctg ctgctgggcg tcatcgccgt gatcatggcg gcgaaggcgc cgcgcggcat
    10561 ctggggcttc gtggagaagc gctgggacat ccacctgttc ccggtgcgcc gcgagctgct
    10621 tctggagtcg ccgccacccg aagaacgcaa gccggccgac aggccggacg ccgccgcgac
    10681 cgagaccgcc tgaggcgggc acggcgccgg ggcgcgccgg ggtccatgcg gagggactcc
    10741 gggcgcgcgg cccgcgcagg actgaccgcc gcgtgagcgg cgacacgacg aggcgctccg
    10801 cttggaccgc cgacacagct cgcgggcgtc gccccggggt gggcctggcc gcgaggatgg
    10861 agggaccgtg atcgagaccg atcgcgacga ggagagagaa cagcgcaacg gagtccaggt
    10921 gctggcgcgc gccgccacga tcctgaggtt gctggcggcc gactcctccg gggggctcac
    10981 gttcagtgag ctagtggcgc gctcaggcct cccgcggacg accgtccacc gcatccgcgg
    11041 cgccctggag cacgaggact tcatctacac cgacgccgcg acgggccggc tgcacctcgg
    11101 ccccggcatc atgcgcctgg cgatggccaa gcgggacctg ccgacggtcg tgcggccgta
    11161 cctcgagcgg ctctcgcgag agctcaacga gaccgtggac ctgggcgtgc tcgacggtat
    11221 gcacgtgctg ttcatcgcgc agcatcccgc gccccagcgg tctctcatgg tcgtctcgcg
    11281 cgtcggcgcg cggttcccgg cctactgcac ggccaacggc aaggcgctgc tcgcgcagct
    11341 gcccgaggac gagatcaagc gccgtctccc caagaagctc gagacgccgg cccggcacac
    11401 gccggtcagc cgcaaggctc tgctggcgga gctcgacgac gtgcgcgcca cggggctggg
    11461 cttcgaccgc gaggagcacc gcgccggcat ctgtggcgtg ggcgtggcca tcaccgacat
    11521 cgacgggagt tcggcgtcca tctcggtgcc catgccggcg gcgcgcttcc acgaggatcc
    11581 ggacaccgtg gccgcggcct tgctgcgcgt gcgcgacgag gtccagatcg cgctgcacaa
    11641 cagctgagcg caggggcagc gggtcgcgca gagcatggac gcccgcccct ccgtcgtcgc
    11701 cgtcatcgcc agcccgcgcc gcggcaacac ggcggcgctc gtcggcgccg cggtggccga
    11761 gctcgaggcc cgcggcgcgg cctgcgagac catcgccctc ggcgacctct tcatcgcgcc
    11821 gtgccaggcg cacgacgggt gcggcgactt cgccgcgtgt cccctgcgcg acgacgcggc
    11881 ggcggtgctg gaccgcgtct acgccgccga ctgcctgatc ctcggcagcc cggtctacta
    11941 cgagaacgtc tccgcccaga tgaaggcgtt catcgaccgt aacgtcttcc gctacgctca
    12001 cgacgaatgg ctgcgggcca cggcagtcgg gctggtcgcg gtcaccgcgg agacggggct
    12061 gcaggagacg ctcgacgcgc tgcgccgcta cgtcgccctg tccacggatc acgacgtgcc
    12121 ggtgttcacc tgcggcgggt acgccgacgc cgccggcgcg gccgccgccg acgaggcgct
    12181 cctggcagag gcccggcgcc tcggcgccga gctcgcctcg gcgctcggcc tcagccgggc
    12241 ctgatcaccc gccgctcggt gacgatggcc gtcaccggga ggtcgtgcgc ctcgccggcg
    12301 atcctcggca cgagctgcgc ctcgaaggcg agcgcgacgc gcggcgtgcc cggccgcgtg
    12361 agcggcaggt aggtgtcgta gaagccgccg ccccagccgc agcggccgcc ctcttcgtcg
    12421 aacgccgagc cggggacgaa caccaggtcg atcgtctgcg gcgggacctc cggcaggccc
    12481 tcgcgcggct cggggatgcc ccacgtgccg ggcgcgaggt cggcggcccg gtcggtgacc
    12541 cggaaggaag ccatgcgccg cgggccgagg atgcgcggca ggcagagcgt cttgcccgcg
    12601 cgcagcaccc actcggcgat gggggccgtg tccagctcgc tgcggaacga ggcgaagagc
    12661 atcacggtgc ccgccgccgc gagctcggga agctcgaggg cgcgagcgcg gaccgcggcg
    12721 gcgaggcgct cgcgcgcgtc ctcgggcagg gcgtcgcgcg cggccagcgc cctgcggcgc
    12781 gcctccgcct tgggcagggc gcgatcgtct ccgcgggact ggtctcgagg ctggctcacg
    12841 ctcgacacgg cctttcgtcg tacactgcgc tggcaacgct tggggccttg ccccagactg
    12901 ggcgtcggct ctctcgccta tagtataggc agagcagccc cgcgggcatg cgggctgctc
    12961 ccgggagggg acagtggcct gcgcggggcg agagacccgt cgcttggccg ccgagaggac
    13021 gaggatggca ctgcgttccc aggaagaggt gtcgctggcg accgtgttcg cgcgcacgct
    13081 gc
//
LOCUS       DASUMX010000157         6388 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_2061821, whole genome shotgun sequence.
ACCESSION   DASUMX010000157 DASUMX010000000
VERSION     DASUMX010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 6388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6388
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2061821"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /gene="cbiB"
                     /locus_tag="VFZ86_07615"
     CDS             <1..753
                     /gene="cbiB"
                     /locus_tag="VFZ86_07615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016880 - acid-ammonia (or amide) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HEX5642193.1"
                     /translation="VLVTAGAAFAAGLALTLVLDRMPWWIALPVGACALKTTFSLRGL
                     VRASRDVQRALASDTDAARASLAALVSRSPDLDPPLIVSATIESLAENLTDSVVSPLL
                     YYALFGLPGALAYRAVNTMDAMLGYRDEREYVGKAAARSDDAANWLPTRIASLLLIAV
                     SAFTGKAGQAWWAFRTQHQPRYGPNKTLAIATMAGSLGVRLEKPGAYTLGTPERPLTA
                     DVIDDAVRRVWAAGGATVALAAGLAALLTWAV"
     gene            756..893
                     /locus_tag="VFZ86_07620"
     CDS             756..893
                     /locus_tag="VFZ86_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642194.1"
                     /translation="MRAVPPARGLAPRPAAQPQDDALERLADHVEAHLDADVLDAVIW
                     A"
     gene            985..1254
                     /locus_tag="VFZ86_07625"
     CDS             985..1254
                     /locus_tag="VFZ86_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014377126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HEX5642195.1"
                     /translation="MCRNIKTLYNFEPPATDEEIRAAALQYVRKISGFATPSAVNREA
                     FDRAVDEVARVSATLLGELVTTAAPKDRETEAARAHARAVRRFGA"
     gene            1262..2140
                     /locus_tag="VFZ86_07630"
     CDS             1262..2140
                     /locus_tag="VFZ86_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HEX5642196.1"
                     /translation="MRIHLVDGTYELFRSHFGAPPRRDAEGREVGATVGLLRSLWLLL
                     STPGVTHVACAFDHVIESFRNGLYAGYKTGAGVDPVLLAQFERAERAVGALGAVVWPM
                     VEFEADDALAAAAARFRDAPGVEQVVLCSPDKDLAQAVVGERVVCWDRRRDVVLDEAG
                     VMRKYGVPPASIPDWLALVGDAADGYPGIPGWGAKSAAAVLARFGHLGAIPDDPAELG
                     LPVARAARLLESLRAGRDDAELFLRLATLRLDVPLEEDVADLEWRGVRPGFAELCAAL
                     GDEGLARRVAEAGRPR"
     gene            2268..2885
                     /locus_tag="VFZ86_07635"
     CDS             2268..2885
                     /locus_tag="VFZ86_07635"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEX5642197.1"
                     /translation="MIGSADKKALVLSALDVDAQHGASTRAILEETLAGRGYAVETVD
                     CRSVDVIPCTGCGSCGLRTPGACAVTDDMQGIFRKMVASDVLVLATPVRFGSYCAELK
                     KVVDRFQPLMVPIYVLRDGEMHFQGRYDLPPLLGVGLVRDGLGPDGAAGGAAAEEADA
                     FRFLIGRLAVNTGTRHAAAAFAGGDERVARAEIQQAVDAVARRTS"
     gene            2882..3547
                     /locus_tag="VFZ86_07640"
     CDS             2882..3547
                     /locus_tag="VFZ86_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEX5642198.1"
                     /translation="MTVLVIEGSRRPKSNTGAIVRDLVSRLAAADRAAEVWRVPRWSA
                     GVEATDEGMEKLRDADIVVLVAPSYLDELPAVTQRLLEDVWERRAELRGHAPLFYGIA
                     HSGFPEPIQRRAELRSMRFVAEQMGWTWMGGVGFGGTSPIDGRPLDEAGMFSKQLRNV
                     LPLVAADIAAGQPFSPETVRRSEKSPFPMPVRMIITLVNSRTRKTARDKQLELDAKVY
                     APD"
     gene            3566..4432
                     /locus_tag="VFZ86_07645"
     CDS             3566..4432
                     /locus_tag="VFZ86_07645"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX5642199.1"
                     /translation="MRGTLRIGEFSALGRVSVRMLRHYEKLGLLEPSQTDAWTGYRSY
                     TLEQLPRLNRIIALKELGFSLQEVGAILAEAPSAGDMRALLSERRARLAAELELSSRR
                     LEQVDVRLAQIEREGVPPGEADVVEKPLPAVTVAAVRGIVPSIDRMSDYCDLHFGRIA
                     EWLGEQGIPADGLTMNLYHMDEYRETDLDVESAMVIDAAVAAGLTGPGAGEVGVRRLA
                     ETPRAASLVLRTGFPGMEDGVMTLLGWVAASGLAMAGPLREVHLFGHPELAAADDPAV
                     LELVVPVAGRDD"
     gene            4540..5478
                     /locus_tag="VFZ86_07650"
     CDS             4540..5478
                     /locus_tag="VFZ86_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017298712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEX5642200.1"
                     /translation="METRPHVIFGTGPVGCWTARSLVDRGLPVRAVNRSGARSGLLPE
                     EVELVRADVADPAQAAAAAEGAATVYQALNPPYHQWKKLFPALQAGALAAASAAGARY
                     VSIENLYLYDSSAVMNEESRVAPVSKKGELRRRMADEVAAAHRDGKVRATQLRSSDYY
                     GPGVTASALGDRVFPPLLAGKKAQVGGSATQPHSFAYIEDVGRAAAELGVRDEALGHA
                     WFAPHASAVTQGDMVAAACRELGREPAVSVVSPAMMRLAGLFVPGARASVEMMYEFTK
                     PFVVDSGRMEREFGLLPTSVDEGIRRTVAWYKAQVG"
     gene            complement(5486..>6388)
                     /locus_tag="VFZ86_07655"
     CDS             complement(5486..>6388)
                     /locus_tag="VFZ86_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX5642201.1"
                     /translation="HFGGADVPLLTVLIPIAYAHRRMTGIPRDGWLAFGRAVERRFLE
                     LGGEIRYGARAEHLETGGGAVRVVPLAGGEALAADRVLSAADGRFTRTVLLGEEDGAR
                     FDPDRVSDQPVQVNLGVAEDWSASGALTYLLPNAPAAAGRAQRRVTVQNKHYDPHAAP
                     PGKSALTVFLESEYCFWKPLEADRHAYEAEKRRCAELVIEAVERHRPGFAGTVEVVDV
                     STPLTRERYTGNWLGAMQAFRPDAGIVRALLQGGPRYDEPGLAGFYSAGQWVESWGGI
                     TTAAQSGRNAVRALCKKDGARFAY"
BASE COUNT          828 a         2320 c         2344 g          896 t
ORIGIN      
        1 gtcctggtga cggccggagc ggcgttcgcc gccggcctcg ccctcacgct cgtcctggac
       61 cggatgccct ggtggatcgc gctgcccgtc ggcgcctgcg cgctcaagac gaccttctcg
      121 ctgcgcgggc tggtgcgcgc cagccgcgac gtgcagcgcg cgctcgcctc cgacaccgac
      181 gcggcgcgcg cgtcacttgc ggccctcgtc agccgctcgc ccgacctcga cccgccgctg
      241 atcgtctccg cgaccatcga gtcgctcgcc gagaacctca ccgacagcgt cgtctcgccg
      301 ctgctgtact acgcgctctt cggcctgccc ggagcactcg cctatcgggc cgtcaacacg
      361 atggacgcca tgctggggta ccgcgacgag cgcgagtacg tcggcaaagc cgcggcgcgc
      421 agcgacgacg cggccaactg gctgccgacc cggatcgcca gcctgctgct gatcgccgtc
      481 tcggcgttca cgggcaaagc cgggcaggcg tggtgggcgt tccgcaccca gcaccagccg
      541 cggtacggcc ccaacaagac gctggcgatc gcgaccatgg ccgggagcct cggggtccgg
      601 ctggagaaac ccggcgccta cacgctcggg acgccggaac ggccgctcac cgccgacgtg
      661 atcgacgacg ccgtccggcg cgtgtgggcg gccggcggcg cgaccgtcgc gctcgccgcc
      721 ggcctcgctg cgctgctcac ctgggcggtg taggggtgag ggccgtgccg ccggcgcgcg
      781 gcctcgctcc ccgtccggcc gctcagccgc aggacgacgc cctggagcgt ctggcggacc
      841 acgtcgaggc gcatctcgac gcggacgtgc tcgacgccgt catctgggcc tgagggattc
      901 gcccgtcgca cctccgggca ccgtcttcag cgtcggtgcg gcgcagcggt gcgccgcgac
      961 tgcccgccag acgaggtgat ccacgtgtgc cggaacatca agaccctgta caacttcgag
     1021 ccgccggcca ccgacgagga gatccgggcg gccgcgctcc agtacgtgag gaagatcagc
     1081 ggcttcgcga ccccttccgc cgtgaaccgc gaagccttcg accgggcggt ggacgaggtg
     1141 gctcgcgtct ccgcgaccct tctcggcgag ctggtcacga ccgccgcgcc caaggaccgc
     1201 gagaccgagg cggcgcgcgc gcacgcgcgc gcggtccgca ggttcggcgc ctgaatccgg
     1261 gatgaggatc catctcgtcg acgggaccta cgagctcttc cgcagccact tcggcgcgcc
     1321 gccgcggcgc gacgccgagg gccgcgaggt cggcgcgacc gtcggcctgc tgcgcagcct
     1381 gtggctgctg ctctcgaccc cgggggtgac gcacgtcgcc tgcgccttcg accacgtcat
     1441 cgagtcgttc cgcaacggcc tgtacgccgg ctacaagacg ggcgccggcg tcgaccccgt
     1501 cctcctggcc cagttcgagc gcgccgagcg ggccgtggga gcgctgggcg cggtcgtgtg
     1561 gcctatggtc gagttcgagg cggacgatgc gctggccgcg gcggcggccc gcttccgcga
     1621 cgcgcccggc gtcgagcagg tcgtgctgtg ctcaccggac aaggatcttg cccaggctgt
     1681 cgtcggcgag cgcgtcgtct gctgggaccg ccgccgggac gtcgtcctgg acgaggccgg
     1741 cgtgatgcgc aagtacggcg tcccgccggc ctccatcccc gattggctgg cgctcgtcgg
     1801 cgacgcggcg gacggctatc ccggcattcc cggctggggc gccaagtcgg ccgccgccgt
     1861 gctggccagg ttcgggcacc tcggcgccat ccccgacgac ccggccgagc tcgggctgcc
     1921 cgtggcgcgg gcggcccgcc tcctcgagag cctgcgggcg ggccgcgacg acgccgaact
     1981 gttcctccgt ctcgcgacgc tgcggctgga cgtcccgctc gaggaggacg tcgcggacct
     2041 cgagtggcgc ggcgtccggc ccggcttcgc cgagctctgc gccgcgctcg gcgacgaggg
     2101 cctcgcgcgg cgcgtcgcgg aggccggcag accgcggtga cgatccgccg atcccctggg
     2161 cggacgcgcg gtgctgccgg cgggccgcgg cgcgtgggta cacgcggcgg accgcgcagg
     2221 gacgagtccg cttgccgccg tcgccgccgc cgggcgatga tgctgtcgtg atcggctccg
     2281 cggacaagaa ggccctcgtg ctctccgcgc tggacgtcga cgcgcagcat ggcgcgtcga
     2341 ctcgggccat cctcgaggag accctggcgg gccgagggta cgccgtcgag acggtggact
     2401 gccggagcgt cgacgtcatc ccctgcacgg gctgcgggag ttgcggcctg cgcacgcccg
     2461 gcgcctgcgc ggtcaccgac gacatgcagg gcatcttcag gaagatggtg gcgagcgacg
     2521 tgctggtcct cgccacgccg gtccggttcg gctcgtactg tgcggagctc aagaaagtgg
     2581 tggaccgctt ccagccgctg atggtgccga tctacgtgtt gcgcgacggc gagatgcact
     2641 tccagggccg ctacgacctg ccgccgctgc tcggggtcgg gctggtgagg gacggcctcg
     2701 ggccggacgg agcggccggt ggagcggcgg cagaggaggc ggacgccttc cgcttcctca
     2761 tcggccgcct cgccgtgaac accggcacgc gccacgcggc cgcagccttc gctggcggcg
     2821 acgaacgcgt ggcgcgcgcc gagatccagc aggctgtgga cgccgtcgcg cggaggacgt
     2881 cgtgaccgtc ctcgtcatcg agggcagccg gcggccgaag tccaataccg gtgcgatcgt
     2941 ccgcgacctg gtgtcccggc tcgcggccgc ggaccgtgcg gccgaggtct ggcgcgtccc
     3001 caggtggagc gcgggcgtgg aggcgacgga tgaagggatg gagaagctgc gtgacgccga
     3061 tatcgtcgtt ctcgttgcgc cgagctacct cgacgagctg ccggccgtca cccagcgtct
     3121 gctcgaagac gtgtgggagc ggcgtgcgga gctccgcggg cacgcgccgc tcttctacgg
     3181 catcgcacac tccggcttcc cggagccgat ccagcgccgc gccgagctgc gcagcatgcg
     3241 cttcgtcgcg gagcagatgg gctggacctg gatgggcggc gtcggcttcg gcggcacctc
     3301 cccgatcgac ggccggccgc tcgacgaggc cggtatgttc tcgaagcagc tgcgcaacgt
     3361 cctgccgctc gtcgcggcgg acatcgccgc cggccagccg ttctcgccgg agaccgtccg
     3421 tcgctccgag aagtccccgt tcccgatgcc ggtcaggatg atcatcacgc tggtgaactc
     3481 gcgcacgcgt aagacggcgc gcgacaagca gctcgagctc gacgccaagg tgtacgctcc
     3541 cgactgacga cgacggaagg aacgcttgcg cggcacgctc aggatcggcg agttctccgc
     3601 gctcggccgc gtctccgtgc gcatgcttcg tcactacgag aagctcggac tgctcgagcc
     3661 gagccagacc gacgcgtgga cgggctatcg ctcgtacacg ctcgagcagc tgccgcgcct
     3721 gaaccggatc atcgccctca aggagctcgg cttctcgttg caggaggtcg gcgcgatcct
     3781 ggcggaagcg ccctcggccg gggacatgcg cgcgctcctc agcgagcggc gcgcgcggct
     3841 cgcagccgag ctcgagctca gcagtcgccg gctcgagcag gtcgacgtgc gcctcgccca
     3901 gatcgaacgc gagggcgtgc cacccggcga ggccgacgtc gtggagaagc ctctcccggc
     3961 ggtgacggtt gccgccgtcc gcggcatcgt gccgtcgatc gaccgcatgt ccgactactg
     4021 tgacctgcac ttcggccgca tcgccgagtg gctgggcgag cagggcatcc cggccgacgg
     4081 gctcaccatg aacctctacc acatggacga gtatcgcgag accgacctcg acgtggagtc
     4141 ggccatggtg atcgacgcgg ccgtggccgc cgggctgacc gggcccggag ccggcgaagt
     4201 cggcgtccgc cggctcgccg agacgccacg cgcggcgagc ctcgtcctgc gcaccggctt
     4261 tcccggcatg gaggacggcg tcatgaccct gctcgggtgg gtcgccgcga gcggcctcgc
     4321 gatggcggga cctctgcgcg aggtccacct cttcggtcac cccgagctcg ccgccgccga
     4381 cgatccggcg gtgctggagc tcgtcgtgcc cgtcgcaggg cgcgacgact gacgcgtcac
     4441 acctctccga tcggcccgtt gactctgaca ctgtgtcagg cctgagcctc ttttcgaggc
     4501 gccggctcag gaaccggtgc atgcgagaag gaggcgccga tggagactcg gccgcacgtg
     4561 atcttcggga ccggaccggt gggatgctgg accgcccgtt cgctcgtcga ccgcggcctg
     4621 ccggtgcggg ccgtcaaccg cagcggcgcg cggtccggcc tgctgccgga ggaggtcgag
     4681 ctggtgaggg cggacgtcgc cgacccggcg caggcggccg ccgccgcgga gggcgcggcc
     4741 acggtctacc aggcgctcaa cccgccgtac caccagtgga agaagctgtt ccccgcgctc
     4801 caggccggcg cgctcgcggc ggccagtgcg gcgggcgccc gctacgtgtc gatcgagaac
     4861 ctctacctgt acgactcgtc ggcggtcatg aacgaggagt cgcgcgtcgc tccagtatcg
     4921 aagaagggcg agctgcggcg gcggatggcg gacgaggtcg ccgccgccca ccgtgacggc
     4981 aaggtccgcg ccacgcagct gcgctcctcc gactactacg ggccgggagt gacggcctcg
     5041 gcgctcggcg accgcgtgtt ccccccgctg ctcgccggca agaaggcgca ggtgggcggc
     5101 tccgcgacgc aaccgcactc gttcgcctac atcgaggacg tcggccgggc tgcggccgag
     5161 ctcggcgtcc gcgacgaggc gctcggccat gcctggtttg cgccgcacgc ttcggccgtc
     5221 acccagggcg acatggtcgc ggcggcgtgc cgggagttgg gccgcgagcc ggcggtctca
     5281 gtggtctcac cggcgatgat gcgtctcgcc gggctcttcg ttcccggtgc tcgcgcgtcc
     5341 gtcgagatga tgtacgagtt cacgaagccg ttcgtcgtgg actccggtcg catggagcgc
     5401 gagttcggcc tgcttccgac gtcggtcgac gagggcatcc ggcggacggt cgcgtggtac
     5461 aaggcgcagg tagggtgagg cgcgctcagt acgcgaagcg cgcgccgtcc ttcttgcaga
     5521 gcgcgcgcac cgcgttgcgg ccggactggg cggccgtcgt gatgccgccc cagctctcca
     5581 cccactgccc ggccgagtag aagccggcca gccccggctc gtcgtagcga gggccgccct
     5641 ggagcagcgc cctcacgatc ccggcatccg gccggaacgc ctgcatcgcc cccagccagt
     5701 tgcccgtgta gcgctcgcgc gtgagcggcg tcgagacgtc gacgacctcg accgtccccg
     5761 cgaatcccgg ccggtgccgt tcaacagcct cgatgaccag ctcggcgcag cggcgcttct
     5821 ccgcttcgta ggcgtgcctg tccgcctcga gcggcttcca gaagcagtat tcggactcca
     5881 ggaacacggt gagcgcgctc ttgccgggcg gcgcggcgtg cgggtcgtag tgcttgttct
     5941 gcacggtcac gcggcgttgc gcccggccgg cggccgcagg agcgttcggc aagaggtacg
     6001 tgagggcgcc ggacgccgac cagtcctcgg ccacgccgag gttcacctgg acgggctggt
     6061 ccgagacgcg gtccgggtcg aagcgcgcac cgtcctcctc gccgagcagg acggtgcgcg
     6121 tgaaccgccc gtcggcggcc gagagcacgc ggtccgcggc gagggcctcg ccgccggcga
     6181 gggggacgac gcggaccgcg ccgccgccgg tctcgaggtg ctcggccctg gcgccgtagc
     6241 ggatctcacc gccgagctcg aggaagcgcc gctcgacggc acggccgaag gccagccagc
     6301 cgtcgcgcgg gatgcccgtc atgcggcgat gcgcgtaggc gatggggatg agcaccgtga
     6361 gcagggggac gtccgccccg ccgaagtg
//
LOCUS       DASUMX010000158         2667 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_48860, whole genome shotgun sequence.
ACCESSION   DASUMX010000158 DASUMX010000000
VERSION     DASUMX010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48860"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VFZ86_07660"
     CDS             complement(<1..272)
                     /locus_tag="VFZ86_07660"
                     /inference="COORDINATES: protein motif:HMM:NF013909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEX5642202.1"
                     /translation="MQVRDVIAAVDRLAPFALAEPWDHVGLQVGAADDELPRGAAGAA
                     DAAPVILVALEVDDAVLDEARRLGAPVVVSHHPLIFDPLERLSDDTL"
     gene            307..1926
                     /locus_tag="VFZ86_07665"
     CDS             307..1926
                     /locus_tag="VFZ86_07665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEX5642203.1"
                     /translation="MNGRSIPPWLVVLALAPGVFLTLADATVMSVAVPLIIRRLEGSV
                     ISVSWVMNGYNLVLTVLFLTMGRLADRYGHKLLFVLGLALFTAASVSCARATSVDALI
                     AWRVVQAVGAAAVVPTALALLLQAYPEGRQGFAAGLFGALSSAAAAAGPVLGGVLVQR
                     WGWPAVFWFNVPVGALGVAMALTLVRRRGGQRSAEPLDWPGVGLVTAGLFCLTLAIIQ
                     GNDWGWTSAVILGLFAAATAILGLWVWWELRTSSPLFDLRLFRRRTFAAASSAIMTVD
                     TALMGTSFMLVIFMIAMMDYTELKAGLAIAVLPAAVLVLTPVAGWLTDRVGPRIPAVT
                     GALVAAAGLVALASLERTDPLSAVLWRSGIVGAGIGLSLPALLAAGMSVVPGGVKGAG
                     SGMLNTARQLGFLLGVAILVAVFAHTMNGAVNSAADKGQALVRADASLSEPVRAKLAG
                     ALDEARTIDATAGMSELRRVAHPIAEDIGKDVGFFEGLRLLELKDVLENLLWDEVSYA
                     FRWPFLVAAIAAALGALAGAFLPRRLQRPTR"
     gene            1957..>2667
                     /locus_tag="VFZ86_07670"
     CDS             1957..>2667
                     /locus_tag="VFZ86_07670"
                     /inference="COORDINATES: protein motif:HMM:NF023702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal domain-containing
                     protein"
                     /protein_id="HEX5642204.1"
                     /translation="MPAQELIARLPERVRDHLANVRENLQLVADLGYADAALAVPDAL
                     GELVVVADARPRTAADPVPATRVSEGLARDDDPEAYDALVERRPVLGTSRRLPGGVTG
                     STDAYPLGLPRPYAVLLRTYGEQTEATASRMEMAFIDAARDLMQTLRGGPLRDARTGG
                     PFSTERRPGDGVLRASPGGDVLYASPNAVSIMRDAGVEGRVTGMRASELPGGALSISP
                     MLGGSGGMETELELGGRTL"
BASE COUNT          303 a         1016 c          961 g          387 t
ORIGIN      
        1 agcgtgtcgt ccgagagccg ctcgagcgga tcgaagatca gcgggtggtg gctgacgacg
       61 accggtgcgc cgagacggcg agcctcgtcg agcacggcgt cgtccacctc gagggcgacg
      121 aggatgaccg gcgccgcatc cgcggcgccc gccgcgccgc gcggcagctc gtcgtccgcc
      181 gcacccacct gcagccccac gtggtcccag ggctcggcga gcgcgaacgg cgcgaggcgg
      241 tcgaccgcgg cgatgacgtc acgtacctgc atgtcgcgaa tactaccccg catatacact
      301 cgccgcatga acggcaggag catccccccc tggctcgtcg tcctcgccct cgcgccgggc
      361 gtcttcctca cgctcgccga cgccaccgtg atgagcgtgg cggtcccgct gatcatccgc
      421 cgcctcgagg gctccgtgat ctcggtctcg tgggtgatga acggctacaa cctggtgctc
      481 accgtgctct tcctgacgat gggccggctg gcggaccgct acggccacaa gctgctcttc
      541 gtgctgggcc tcgcgctgtt cacggcggca tccgtgtcct gcgcgcgcgc cacgagcgtg
      601 gacgcgctca tcgcctggcg cgtggtgcag gccgtgggcg ccgcggccgt ggtgcccacg
      661 gcgctggccc tgctgctcca ggcgtacccg gagggccggc agggcttcgc cgccggcctc
      721 ttcggcgcac tgagctccgc ggccgccgcc gccggtcccg tcctgggcgg cgtgctcgtc
      781 cagcgctggg gctggccggc ggtgttctgg ttcaacgtgc cggtgggcgc gctcggcgtc
      841 gcgatggcgc tgacgctggt gcgccggcgc ggcggacagc gctcggccga gccgctcgac
      901 tggcccggcg tcggcctcgt caccgccggg ctcttctgcc tcacgctggc gatcatccag
      961 ggcaacgact ggggctggac ctcggccgtc atcctcggtc tcttcgccgc cgcgaccgcg
     1021 atcctcgggc tctgggtgtg gtgggagctc cgcacctcct ccccgctctt cgacctgcgg
     1081 ctcttccggc gacggacctt cgccgccgcg agctcggcga tcatgaccgt ggacacggcg
     1141 ctgatgggca cctcgttcat gctcgtcatc ttcatgatcg cgatgatgga ctacacggaa
     1201 ctgaaggcgg ggctcgcgat cgccgtcctg ccggccgcgg tgctcgtgct gacgccggtc
     1261 gccggctggc tcaccgaccg cgtcgggccg aggatcccgg cggtgaccgg ggcgctcgtc
     1321 gcggccgccg gcctggtcgc gctcgcctcc ctggagcgga cggacccgct gagcgccgtc
     1381 ctgtggcgca gcgggatcgt gggcgccggc atcggactct cgctgccggc gctgctcgcc
     1441 gccggcatga gcgtcgtccc cggcggcgtc aagggcgccg gctccggcat gctcaacacg
     1501 gcgcggcagc tcggctttct gctcggcgtc gcgatcctcg tggcggtgtt cgcgcacacc
     1561 atgaacggag ccgtgaacag cgctgccgac aagggccagg ccctcgtgcg cgccgacgcc
     1621 tccctgagcg agccggtgcg cgccaagctc gccggcgcgc tcgacgaggc gcgcaccatc
     1681 gacgcgaccg cgggcatgag cgagctgcgc cgcgtcgccc accccatcgc cgaggacatc
     1741 gggaaggacg tcggcttctt cgagggcctg cgcctgctgg agctcaagga cgtgctcgag
     1801 aacctgctct gggacgaggt ctcgtacgcg ttccgctggc cgttcctcgt ggcggcgatc
     1861 gccgcggcgc tgggcgccct ggcgggcgcc ttcctgccgc ggcgcctcca gcggcccacg
     1921 cggtagctac gcgccccgca cgtatactgt ggcccggtgc ccgcgcagga actcatagca
     1981 cgcctcccgg agcgcgtccg ggaccacctc gccaacgtcc gggagaatct ccagctggtg
     2041 gccgacctgg ggtacgcgga cgcggcgctc gccgttcccg acgccctcgg cgagctggtc
     2101 gtggtcgccg acgcgcggcc gcgcaccgcg gccgatcccg tgcctgccac gcgcgtgtcc
     2161 gagggtctgg cgcgcgacga cgaccccgag gcctacgacg ccctcgtcga gcgccggccg
     2221 gtcctgggga cgagccggcg cctgcccggg ggcgtcacgg ggtccaccga cgcctatccc
     2281 ctgggactcc cccggcccta cgcggtcctg ctgcgcacct acggcgagca gaccgaggcc
     2341 accgcaagcc gcatggagat ggcgttcatc gacgccgcgc gggacctcat gcagacgctg
     2401 cgcggcgggc cgctgcgcga cgcgcgcacc ggcgggccgt tctcgaccga gcgccggccc
     2461 ggggacggcg tgctccgtgc cagcccgggc ggcgacgtcc tctacgcctc gcccaacgcg
     2521 gtcagcatca tgcgcgacgc cggcgtcgag ggcagggtga ccgggatgcg cgcctcggag
     2581 ctgccgggcg gggcgctgag catctccccc atgctcggcg ggtccggcgg catggagacc
     2641 gagctcgagc tcggcggccg cacgctc
//
LOCUS       DASUMX010000159        42401 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_193688, whole genome shotgun sequence.
ACCESSION   DASUMX010000159 DASUMX010000000
VERSION     DASUMX010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 42401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42401
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193688"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="VFZ86_07675"
     CDS             <1..345
                     /locus_tag="VFZ86_07675"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014901279.1"
                     /note="catalyzes the formation of L-citrulline from
                     carbamoyl phosphate and L-ornithine in arginine
                     biosynthesis and degradation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEX5642205.1"
                     /translation="ARAQADAVATGATVELTSDLKAAVQGADVIYTDTFTSMGQEAEH
                     DVRLAALRDYQVNMDLVKLAGPQVKVMHCLPAHWGEEITEEVLYSESSVVFDQAENRL
                     HAQKAIMAGLIA"
     gene            complement(446..2053)
                     /locus_tag="VFZ86_07680"
     CDS             complement(446..2053)
                     /locus_tag="VFZ86_07680"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX5642206.1"
                     /translation="MLARPLRIADPDRRHGYALAVALVLLVSTALRLWQLSTPAEMMF
                     DEIYYAKDGKAIVDGRVGTDGQYRWAAGDEVSWPHPEMGKFAIAGGILLFGDRSFGWR
                     LPAVIAGVALLACVYPLGRRLGLSPPWALLALIFAAADPLGVAQSRIATLDIFIAAWT
                     VLCVLLTLRYVQDGWRPRWLFAAGAAGGMALATKWSGGLALFAAALIIVVAWLLQRRA
                     ARRAASAEDDGATRAAAPGDEVGPPAVVPHAEAVVAAPPQGSAARRIAVIAAALLALP
                     VAVYLLSYAQYFATGHTLADFRELHRQMITFNLNLQATHTYASRAFTWIVDYRPVWYY
                     FEGTKTYRGVIAIGNPFLWWLATLSLAAAVVLAVLRRSYALLPAAAIVVALYVPWFLT
                     TRTSFLYYMTPVAPFMAILVAAALCLFAGGVLPRRGWFAAAAAALAAAILWRPLGIGA
                     GWLFWSLPRRAGETAGWVGVAVGVALALAVLVFLLSSRMRAYRPWTAMVVAGLAIGIV
                     VAFVPIVLGLPISPKYFEQIMWFPSWI"
     gene            2323..2898
                     /locus_tag="VFZ86_07685"
     CDS             2323..2898
                     /locus_tag="VFZ86_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HEX5642207.1"
                     /translation="MATLRDVTANPGPLGLMAFGMTTILLNIHNAGFYEMNTMILAMG
                     IFYGGIAQVIAGIMEWKKNSTFGMTAFLSYGLFWLTLVGLIVFPKWMGVDGPDDVAMG
                     WYLMAWGVFTGLMFIGTLRINRSLQTVFLSLTVLFVLLAVGDWTGNATITKIAGWEGI
                     FVGLSAVYGSIAQVWNELYGKVVLPLGPYKK"
     gene            3110..3721
                     /gene="lexA"
                     /locus_tag="VFZ86_07690"
     CDS             3110..3721
                     /gene="lexA"
                     /locus_tag="VFZ86_07690"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002199634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEX5642208.1"
                     /translation="MEGLTPRQEQVLEFIRASVRADGYPPTVREICAALDLSSPSTVH
                     AHLANLERLGLIRRDPSKPRALDVVGDARPPRPLPLIGSVAAGAPITAVDNVEDLVDV
                     PGFLRRDDDDFVLRVRGDSMTGAGIFDADLIVVHPQPDARNGEIVVARVAGIADTEAT
                     AKRFFREGAVVRLVAENENYEPIVVNADQVELVGRVVGVLRQL"
     gene            3727..3951
                     /locus_tag="VFZ86_07695"
     CDS             3727..3951
                     /locus_tag="VFZ86_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642209.1"
                     /translation="MPLATAVQTSLADAVDESLAAALAGRDEPCLWCGGHSVRVLGAD
                     LWSGAVTVSCPTCGSELSGVVPRRLREVPR"
     gene            4062..4340
                     /locus_tag="VFZ86_07700"
     CDS             4062..4340
                     /locus_tag="VFZ86_07700"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEX5642210.1"
                     /translation="MAGVLRFAVFLFLIFVAVWAGVRVAHAGDDPAIYSGTPYTVAAG
                     DDLWTIAQAKYGGESDLRAAVYAIRDANDLETSVLQPGQDLTLPYLGD"
     gene            4455..4531
                     /locus_tag="VFZ86_07705"
     tRNA            4455..4531
                     /locus_tag="VFZ86_07705"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4489..4491,aa:Pro,seq:tgg)
     gene            4544..4619
                     /locus_tag="VFZ86_07710"
     tRNA            4544..4619
                     /locus_tag="VFZ86_07710"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4577..4579,aa:Gly,seq:tcc)
     gene            4666..4742
                     /locus_tag="VFZ86_07715"
     tRNA            4666..4742
                     /locus_tag="VFZ86_07715"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4700..4702,aa:Arg,seq:tct)
     gene            4963..5214
                     /locus_tag="VFZ86_07720"
     CDS             4963..5214
                     /locus_tag="VFZ86_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642211.1"
                     /translation="MGDMIHLPPNVTGDTKEQAFGYGGTRTPFRVEECSHVYAREKSE
                     KIDVLDPSPARHVKTLIFVRRLTPEEVYDQRGWEREAGV"
     gene            5454..6221
                     /locus_tag="VFZ86_07725"
     CDS             5454..6221
                     /locus_tag="VFZ86_07725"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HEX5642212.1"
                     /translation="MSDRDDIDISSRGASADAVYAAVAVALADHDKATAVRTAVEAVT
                     AGAVSIPELYRDVLARILADTGAAWQTGKTAIWEEHLASSTVRTIVEILYPGVLKAKA
                     AVAPAGRSVLLACPPDEGHDLGLRMVADRFDMAGWTTFFLGPDTPVAEIADAARRLGV
                     DAVVMSSSTHFHRVAVRHAVDVLKKELPGTDIWVGGPAFVGAATGWLPQEVVDLDALL
                     GGPAPGADVANEGAATDAADDGADGADDTVTASGEEG"
     gene            6223..7374
                     /locus_tag="VFZ86_07730"
     CDS             6223..7374
                     /locus_tag="VFZ86_07730"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX5642213.1"
                     /translation="MLGFRIAIRFLRKSPVQSILIAAGIAVGIGVQVFLGSLITSLQA
                     SLVDQTIGNSPQVVVTAGQDGAAVAYTPQLQQALEAQVEITTVVPTRAFSAIYSSGRE
                     NAPLQVTGGALDELDTIYDITGRLVAGEASLDSDEVIVGKDFADKYGLRPTDQFPVTL
                     PSGEQAGFVVSGVFDFGSAAANSATAFVNGDRAAEVLGLAADEFSAVDAQVDDVFSSE
                     KVATDLRGQPALEGLTVTEWQAENADLLSALQSQSSSSYMIQFFVLVAVALGIASTLA
                     ISAVQKTRQIGILKAMGMKDRQSGRIFLWQALILGVSGAAVGVAAGLGLIALFTFLGR
                     DSESLFPITPQVGFVVLSFVVGVLVAVVSSFIPSRRTSKLDPIEVIQGG"
     gene            7367..8095
                     /locus_tag="VFZ86_07735"
     CDS             7367..8095
                     /locus_tag="VFZ86_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5642214.1"
                     /translation="MAETLVHAEGIGKTYGTVVKTVALHEVSFEIAAGEFASVIGQSG
                     SGKSTLLNLIGLLDTPTTGRIVLNGHDATAASRKQRAALRNDFIGFVFQFHYLLPEFS
                     VLENVLMPGRIAGAAPSDLVPRAEENLALLGLEGLEEKNANDLSGGQKQRVAIARALM
                     NRPALVLADEPTGNLDTVNTAAVYRLFREINEEQGTAFIIVTHDRGVAQQTDRILEIS
                     DGALLQDVRNEYLAPDATPSPERV"
     gene            8181..8256
                     /locus_tag="VFZ86_07740"
     tRNA            8181..8256
                     /locus_tag="VFZ86_07740"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8214..8216,aa:His,seq:gtg)
     gene            8311..8396
                     /locus_tag="VFZ86_07745"
     tRNA            8311..8396
                     /locus_tag="VFZ86_07745"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8345..8347,aa:Leu,seq:tag)
     gene            8493..9854
                     /gene="tig"
                     /locus_tag="VFZ86_07750"
     CDS             8493..9854
                     /gene="tig"
                     /locus_tag="VFZ86_07750"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEX5642215.1"
                     /translation="MKTKVNPTGENEVELSVEVPAETVRKAYDRAVAKIREEMQLPGF
                     RKGRVPVNLVVQNVGADFIRGEALEDAIPEWGDEALKAAGLYDDAVGTSDLQVGPLDE
                     TQDYAFSLKVQTMPVPALGEYKGLEVPKREVTITEDQISAQLAMLQERLASLQPVEDR
                     AVQTGDFVLMDLAGSRDGEPIEGAQGKDQMYEVGRGNLIPGFEEELVGVKAGEEKSFD
                     VTFPEDYHAEELAGQAATFTVAVKEIKEKVTPELDDAFAADVSEFETIEELRNDVRER
                     LQAAADTGAQREFRAAAVEKAVEHATLAVPSSMIDREAHRLYHQLEERVGEQGLTMEV
                     YLGVLEKTEEDIEEEMRPQAEFIIKRQLVLEAIAQAEALEVTDEDITEVVKHDAEALG
                     RDYLQLLADLEKSGRREALRDEMLVAKAADFVADAAVAVPMTEAEEAAAEESADAVDP
                     EDD"
     gene            9913..10497
                     /gene="clpP"
                     /locus_tag="VFZ86_07755"
     CDS             9913..10497
                     /gene="clpP"
                     /locus_tag="VFZ86_07755"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEX5642216.1"
                     /translation="MTPLVPFVIEQTSRGERSFDIFSRLLNERVIFLGTPVVDEIANL
                     IVAQLIHLESDDPDKDISLYINSPGGSVYAGLAIYDTMQFIKPDVSTICVGSAMSMGA
                     LLLAGGAKGKRFALPNARVLIHQVAGGYEGQATDIEIHAREVLNLRKRLDEIIADHTG
                     QEIDKVRSDTERDYFMSAAEAREYGIIDDVIAHR"
     gene            10535..11806
                     /gene="clpX"
                     /locus_tag="VFZ86_07760"
     CDS             10535..11806
                     /gene="clpX"
                     /locus_tag="VFZ86_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEX5642217.1"
                     /translation="MAGTTDGNDQLLCSFCGKSQRQVKKLIAGPGVYICDECIDLCNE
                     IIDEELTAPATLDLEQLPKPRDIYGILNDYVVGQDEAKRSLAVAVYNHYKRVQAVSSG
                     ESDVELAKSNILLLGPTGCGKTLLAQTLARILNVPFAIADATALTEAGYVGEDVENIL
                     LKLIQAADFDVKRAETGIIYIDEIDKVARKADNPSITRDVSGEGVQQALLKILEGTVA
                     SVPPQGGRKHPHQEFLTIDTTNVLFICGGAFSGLEKIIERRMGRQGVGFMADVVSKAD
                     KDPGTIFTHSLPEDLLDFGLIPEFIGRLPVVSAVHNLDENDLMRILTEPKNALTRQYQ
                     KFFSLDGVELVFAEESLTAIATKALARGTGARGLRSIIEESLLGVMFDLPSRTDVRKC
                     VITKETIEKGIDPTLLTEADKSLPERDAESA"
     gene            11862..14486
                     /gene="lon"
                     /locus_tag="VFZ86_07765"
     CDS             11862..14486
                     /gene="lon"
                     /locus_tag="VFZ86_07765"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEX5642218.1"
                     /translation="MPTRELVLFPRMVAPIFVGRERSISAIEAAFSGQTALILCAQRE
                     AAVEEPSFDDLRPVGVRARVLQLFKLPDGSVKALVEGDERVRTERLVASDPHFLVSYA
                     PVESTGVGTARARSLARRVTDEFVRYVQLHPQLADEAQFVVQQAADADELADIVAAHL
                     QTETGEKQALLEIVDTQQRLLALLESLAEENAVLGIEQEIGLKVQQRIEGAQRQMLLH
                     EKLRVLRDEIEEAGESADEDLAGYLERAAEAGLSPAARKLVTRELTKLRQAPPMSPEI
                     AVIRGFLDTVLELPWGRLAPADVDVPGVARHLERTHDGLKDVKDRILEYLAVCRLRAD
                     ASRKTGEPADAADQPATILCLAGPPGTGKSSVAQSIADALGRPFVRVALGGVRDEAEI
                     RGHRRTYIGAMPGRIVDGLAKAGVDNPVMLLDELDKLDSDWRGNPAAALMEVLDPAHN
                     HAFRDNYLEAEYDLSRVFFIATANDVDAIPATLYDRLEVIHLSGYTLAEKLSIARRHL
                     LPRVARDTGLRRGDVRFGRGALPAIVRGYTREAGVRELERALRRVDRKLARRHLEEGL
                     AFPATVAAAELRGYLGEAHWLEAELPRAASVGESLGLAYTSDGGEVLTVEATLARGRG
                     ELVLTGQLGEVMQESASAAWGYLLSHAERDRGLAPLVGRSPFMTADGLDLSGQDVRVH
                     VPEGAVPKDGPSAGLALAVAMLSALGGVPVKPRVAMTGEITLGGRILRVGGLKEKLLA
                     AGRSGVRTVILPSSNRGTVEELSPDLTDKLELVYVASFEEALPHVFGRRVFRERASKG
                     QGKRVTGSMGAANMHDHDHEHDHQTPGSTEEPGVDAGSGQLEQHCSQCGSLIGGSDEF
                     CQVCAIEYSGGEAPEEEK"
     gene            14772..17396
                     /locus_tag="VFZ86_07770"
     CDS             14772..17396
                     /locus_tag="VFZ86_07770"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEX5642219.1"
                     /translation="MTDPSSGTDYLQQTTRFEPAAVEARWMKRWLDEHLFHAEPDPDR
                     PAYSIVIPPPNITGVLHMGHALNDTVQDVLTRYHRMLGENACWLVGTDHAGIATQNVV
                     EKMLRKEGLTKEDLGREEFVARVWEWRREYGGTIIEQLKRLGSSCDYERERFTFDEGY
                     VRAVTRVFVALYDKGYIHKDSYLVNWCPRCGTAISDLEVAYRDMPGTLNHIRYEIAGG
                     GSVTIATTRPETMLGDTAVAVHPDDERYTAVVGKTAVLPLLGRELRIVADERVDPSFG
                     TGALKITPAHDLTDFDIGRTHGLETVQAIGQDGRITAAGGPYAGLEVEEAAARVTADL
                     RALGALVKVEDYPHSVATCDRCGTPIEPLISEQWFMDMDELKKPATDVVRDGTVTFTP
                     ERWGRVYIDWMDNLRPWCISRQLWWGHQLPVWYCPDGHLTVAETEPQACATCGSTELT
                     RETDVLDTWFSSALWPFATFGWPEQTADLAYFYPTNVLSTGRDIIFLWVARMIMMGLE
                     FMDEIPFDDVYIHSVIQAPDGRRMSKSLGTGIDPLDVIDEHGADATRFGLLLMSSTQD
                     VRFSDEKIAMGRGFANKLWNAARFVLLGTQGYAAERSDVAEVDRWITSRFQRCLAAVE
                     EAVAVYDFTAAVDALYHFVWDEFCDWYVELIKVPLYGEDEAKKAQVAGHARWMLDQIV
                     RLLHPFMPFVTEEIAEQYGAAPLLRQQHPRRDEEQLAADDEKAIGELQAAVDALRRFR
                     AEAEIPPGKVLAAVFAGDAAAAERYEHHAGVFRAMARTTLAFAGEPETDATVVVVPGG
                     RLFVAAAIDKAEEIARLEQQLARAQAEVERGEAKLGNEKFVGRAPEAVVTKEREKLAS
                     HVAERDGLAARLAQLRGG"
     gene            17410..18732
                     /locus_tag="VFZ86_07775"
     CDS             17410..18732
                     /locus_tag="VFZ86_07775"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEX5642220.1"
                     /translation="MSADAGNLEWDAYLESLAAFGMRPGLERVTELLERLGRPQDAYS
                     VIHIVGTNGKSSTTRYAEALLRAHGRRSGAYLSPHISGWTERVIVDGRPVEEPVFAAA
                     VERVREEIPALPRGLGETTQFEVLTVAALLAFARSGVEAVALEAGLGGRLDATNVVKA
                     PVVVLTNIALEHTEVLGGTRALIFAEKAAVIKGGDVVFGDLDGLEGEARRVCSATGAT
                     PHFLRESASQGDLAVVGSPEDFTVTFAPGDRPERWAGLRVPTPALYQVRNAALALAAA
                     RLLLGGLDEAAARRALAGTAVPGRLQVAGERPLVLADGAHNPDGVRVLAQSLAAVRMP
                     RPAVGVLAIMRDKDYRGMLDGFLPLLDHVVCTQATEPRSLTADELATAVHASARARGG
                     RPPAVATIRDAHAALARARELAGEAGSVLIGGSLYLLEDLSDVLAAGL"
     gene            18789..18932
                     /locus_tag="VFZ86_07780"
     CDS             18789..18932
                     /locus_tag="VFZ86_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642221.1"
                     /translation="MAQQRAHPVVRFLVALLIVVAVAAVAFFVGYLIGMRLGAGVALP
                     VTF"
     gene            18972..20453
                     /locus_tag="VFZ86_07785"
     CDS             18972..20453
                     /locus_tag="VFZ86_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HEX5642222.1"
                     /translation="MTDPASRDQLILVTGATGYVGGRLVPRLLAAGRRVRVLARDPRR
                     LEGRDWAERVDIVAGDVLRPESLAAALDGVGVAYYLVHSMAKGEGFHERDLVAARAFA
                     SAALAAGVSRLVYLGGLGDPAADLSRHLRSRQETGEALREAGVPVTEFRAAVVVGSGS
                     ISFEMIRYLTERLPVMVCPQWVYTRVQPIAVDDLLRYLVAALDVPESTGRVVEVGGTD
                     VLTYGEMMLGYARARGLRRRLQPVPVLTPKLSSYWVHLVTPIPSTMARPLIEGLRNET
                     IVREQGAHDLFPAIHPVGYDAAVRAAVASLDTGEVETSWADALVTSGGDVQPRVLTTK
                     EGKLIERRQALVAATPEETFAVLQTIGGRRGYRAWDWAWELRGAADRLVGGVGLRRGR
                     RDPDEVRVGDALDFWRVEAVEAGRLLRLRAEMKVPGAAWLQFETLPHDGGTLLVQTAY
                     FAPRGVPGLAYWYALLPVHSRIFSGMIAALAAEAGRPAAPAGG"
     gene            20534..21064
                     /locus_tag="VFZ86_07790"
     CDS             20534..21064
                     /locus_tag="VFZ86_07790"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX5642223.1"
                     /translation="MPFWTIVAANSTTTTSDSSNPFQSVLDFFESPTWQFLTMLFFFF
                     LVVLWIACAYWVFKDARRRIDDKIVLTVAVLTGIVFGPIGLIVYAIVRPPETLADRRE
                     HELEVQMMEQRIGEEERCSFCKTPVREDYLVCPTCARRLRTSCPSCRKPIDPRWRVCP
                     YCEADVHAMPPVFDRV"
     gene            21133..21546
                     /gene="ndk"
                     /locus_tag="VFZ86_07795"
     CDS             21133..21546
                     /gene="ndk"
                     /locus_tag="VFZ86_07795"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003249570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEX5642224.1"
                     /translation="MERTFIMVKPNGVTRGLVGEVVARFERRGFALRGMKALRIDRAL
                     AEKHYAEHVGKPFFESLVAFITSGPVVAMVWEGREAISVARAMMGVTDSAQAAPGTIR
                     GDYSLSKEENVVHGSDGPESAAREIALFFGESDLV"
     gene            21539..22207
                     /locus_tag="VFZ86_07800"
     CDS             21539..22207
                     /locus_tag="VFZ86_07800"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEX5642225.1"
                     /translation="MSDPVPQHFWERVLPDRPGEPSVGFGGLVLASRSPQRKLLLEQL
                     GLPFRVAVSEHDEGVSDGDPAHTVQQNARGKAEDVMARETVRAGELVLGVDTVVVCEG
                     EILGKARDEAEAAAFVRRLAARRHEVYSGVYLTSRKLALATHVVTGVTFRSLTDADLD
                     AYIASGEWRDRAGAYAIQGIGSGLVRHVDGDYFNVVGLPIAELMRLLASFGVAPFSWL
                     PGQV"
     gene            22422..23465
                     /locus_tag="VFZ86_07805"
     CDS             22422..23465
                     /locus_tag="VFZ86_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX5642226.1"
                     /translation="MSFLGFVTGFGARDMAVDLGTANTLVYLRGKGIVLSEPSVVAID
                     HRSGEVHAVGAEAKRMLGRTPATISAVRPLKDGVIADFDVTEQMLRHFIRKVMQNRWA
                     HPRVVICVPSGVTGVEKRAVEEATYSAGARWATLIEEPMAAAIGAGLPVAEPTGSMIV
                     DIGGGTSEVAVISLGGIVVSQSIRVGGDELDEAVISYVKKEYKLLIGSQTAEEIKLEI
                     GSAYPLPQEEQAEIRGRDLVTGLPKTVVLSSEEVRTALEEPTQAIVDAIKATLDKTPP
                     ELASDIMDRGIMLAGGGSLLQGLAERLRDETQMPVHVAESPLTCVAVGSGKYLEEFDV
                     LVKSKRNSRPRGR"
     gene            23521..24315
                     /gene="mreC"
                     /locus_tag="VFZ86_07810"
     CDS             23521..24315
                     /gene="mreC"
                     /locus_tag="VFZ86_07810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEX5642227.1"
                     /translation="MAVLLLLAVALLTAYFRESDSGVAHSVQGGVLTVVSPLQSGTAT
                     ATKPFRDAWNWVGDLFGAKSENTALKKEVQDLRALLAEELTTQAENEQLRALLDFQED
                     RVYPKDVQLVTARVVARSTSAWYSTATISAGSSDGVDVYDTVVNGQGLVGRVTKVNAN
                     AAQVLLVTDQQGFVDAMVEPGGAQGLTSGSVTGDVTLEYVDKNEKVKVGQYVVTSGRN
                     GSVFVRGIPIGQVESVVQQDVELYQSIAIKPFVDFHKLDIVQVITR"
     gene            24312..24872
                     /gene="mreD"
                     /locus_tag="VFZ86_07815"
     CDS             24312..24872
                     /gene="mreD"
                     /locus_tag="VFZ86_07815"
                     /inference="COORDINATES: protein motif:HMM:NF016019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEX5642228.1"
                     /translation="MSVIRPVAEKTERAWPAWGEVARLAVVLLLAVLLQTAVAPHLRL
                     LGAYPDFALIAVVCVALVKGSETGAVFGFLTGMLTAIALMEPFGLSAFVFVLVGYFAG
                     RYAETADLPAGLAPLLTVFAATLVANVLFAMSQFLLAREVPLGFFLGRVLFPSLVFNT
                     LLAAPVYLLVRVWLRGAGDLRAAART"
     gene            24853..26901
                     /gene="mrdA"
                     /locus_tag="VFZ86_07820"
     CDS             24853..26901
                     /gene="mrdA"
                     /locus_tag="VFZ86_07820"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX5642229.1"
                     /translation="MPLLGRDPDRRDRPIRTGRRDKPIIQLPSVALRVAVILGLAAVL
                     FGVVFFRLWFLQILSGQEFVAQANDNRLSSVKVVAPRGNIVDRKGEVLVENRAGTAVG
                     IRLMAVPQGQLDAEIERLAGVLKMRPGKIRKEIMDYLRPSTMEQDEDGTWTSFLTWRK
                     VLDGDITGLDLIVVKEDVSKGVRSYLKEHTLDFPGVEVRDQYLRSYPQGDMAAQLLGH
                     LGEIDADELKDQHFKGYAAGDVVGKDGLEDTYDAWLRGRDGVARIEVDAYGRPKQTEP
                     VGGRMAEPGHTLVTTLDVDVQAAAEEALRTGISLAHKDGKYAANGGAAVVLDVRNGEI
                     LGMASYPTYDPGVWVGGISTKDYEQLTRKSANNPMINRPISETKAVGSTFKAVTAVAA
                     LEEGHISSGTGYFCPGSYSSMYDNADPPQKFKCWSDGGHGTLDLVGAITQSCDVYFYN
                     VGDRFYGRQGSEALEDWAKRFGMGKVTGIDIPGESAGRVPTPGWKKSYFETEIDKIWK
                     PGDSVNLSVGQGDLEASPLQLAVSYAAVANGGKVVTPHLGLEVVDAAGQTVRTLEPAP
                     PRKVDISQSTLDVVRRGLYEAAHNGAGTSAAVFQNYDVAVSGKTGTAEVWDDTSKHYV
                     DYAWYASYAPSEDPRYAVVVLIEKGGHGSTAAAPATRLIYDALFGIDGGAFTGAPRGD
                     "
     gene            26986..28134
                     /gene="rodA"
                     /locus_tag="VFZ86_07825"
     CDS             26986..28134
                     /gene="rodA"
                     /locus_tag="VFZ86_07825"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEX5642230.1"
                     /translation="MVDWRAYFRHLDYLLILATAGLIVYGVTMIYFATRHDIDGAPLY
                     YVRQQLIAVAMGLVAAIVASLLDYEIYRRFQWLLYAAAVLIVVAVLPLGENVNGATRW
                     IDLGFTRFQPSAAAMLLLALSIGAFLADRMELLGSKRITVIVLVLAAVPAFFVYQEPD
                     FGSASVIMVLALAMLFFYGTPWTHFAVLTATGVTAFVVALKVLPLAGVELIKQYQVDR
                     LFVFINPGHDSAGTGYNVIQSMIAVGSGALTGRGDQATQTTLDFLPEHHTDFIFAVIS
                     ERNGFVGASILLVLYALFIWRALRIAVMARDMYGSILAGGIGVMVLFQVFVNIGMTIG
                     IMPVTGIPLPFISYGGTAMIAFLIFVGLLQAIHLRATITPESRGRLGQ"
     gene            28124..29044
                     /locus_tag="VFZ86_07830"
     CDS             28124..29044
                     /locus_tag="VFZ86_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642231.1"
                     /translation="MASSLSISKILKAVTDARGSVDASRVCVVGVGDDLARVVGVLSA
                     GARDESGGVSAKVDALSPADFPREQRLAARWDIVVLVAAGCPAAEASPVVHAARRGGC
                     KVIGLVEEPGEAAWAREAGLFDDEVARGLGYDAAGKPTLERKIVEAAGDGAAALAAAL
                     PSVRRTYCDHVVITNAAQNGVIGVVVIIPGADMPAMTANQIRMVLKIAHAYGEEMGID
                     RAVEILSIVGTGFVLRTLARQALDFVPGFGWAVKGAVGFSGTVALGQAAIAYFEAGAP
                     LRLSRMQRINRQVEKLTSRLPGPLQRLASG"
     gene            28989..30758
                     /locus_tag="VFZ86_07835"
     CDS             28989..30758
                     /locus_tag="VFZ86_07835"
                     /inference="COORDINATES: protein motif:HMM:TIGR03960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HEX5642232.1"
                     /translation="MKSSRPGFPGRSNDWPPDEVARLLARVTRPGRYVGGEFNTRLKP
                     GARPRIVLSYPDVYEIGVSNPALQILYSHLNDATPASAERAYCPWPDMADLMRAAGVP
                     LWTLETFAPVGECDLWGFTLPHELCYANVLEMLDLAGVPLRAAERDGGDPIVLGGGPA
                     VANPWPLAPFFDAFFIGEAEDHLDAIAEAMSADGRERRLRALAAVPGVWAPGRSDGPA
                     ERQVFMGFSAAMPVLRPVVPVLEAVHDRVVVEVMRGCTAGCRFCQAGMWYRPVRERPV
                     DLIVTAADRLLDETGCDEVSLLSLSSCDYGGVAEALTRIRELRPGLRVSLPSLRIDSA
                     AQSLASFAAGQRGSLTLAPEAGTQELRDAINKGVDEAQFEEAVRATFAGGFTGLKLYF
                     MIGLPGETDDDVLGIAGMAGTAARLAREIGRGRARLSVAVSSYVPKAHTPFELEPFAG
                     ESVLRRRQQLVREAAPRNVRVAFHDVAASVVEATLARGGPGTDRLVEEAWRRGARFDG
                     WSEHFDVRRWEDAAASTGVALGERTVERAFWRDAVDARLHEDFFADELARSRQGDLTE
                     DCRDGACAACGVCAGEVGMDLLA"
     gene            30755..31435
                     /locus_tag="VFZ86_07840"
     CDS             30755..31435
                     /locus_tag="VFZ86_07840"
                     /inference="COORDINATES: protein motif:HMM:TIGR03936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HEX5642233.1"
                     /translation="MSWWLIAFARRGTARYLSHLDTARTVQRTFARAGVPIALSHGMR
                     PKPRLSLPLPLPVGAAGDEELAVVEVPEGVDASAVTLKALRAASPPGFEPLSIVDAGE
                     QRPHPQPRAAEYACLLDGDAGTIVAAVERYNDAEDAIRERVSPKGTRRLDLKHYAGDV
                     GATPVEGGARLGFTIHHRRDGAARPQELIDLIAEWAEVEPAMRGLQRLRIAWDDRPAG
                     LRAAGGRS"
     gene            31440..34193
                     /locus_tag="VFZ86_07845"
     CDS             31440..34193
                     /locus_tag="VFZ86_07845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEX5642234.1"
                     /translation="MSTAKKGTPGDEPAATPTGQNGAGEGQETTGAAGEAAPRRRRGN
                     RGGRRHKRPATAAGDAGQAQATAAADASQAQTPAQTPRDGYAAATDALASLPTAPLIP
                     VGAAEAAARDKVAADEAARGDASPGGERAPAGEARAPRGRSRGGRGRARPSAAATETP
                     VVAPVAAPEPEKAADEPAAGAAADATPAGEEEETHAKRRRRGGRGGRRRRKTAEIEHE
                     EATREAEEAAGEPLASGALAEEAPAPAPAAEVAGQPAGERADGAQAGEAGAKRRRGAR
                     GGRRTRGKAEAAAAEPAEASEAAEPAAEPGSVAGPAKAPAAEPKTRRPRDRRRPRAIV
                     RQDEGSGLDTIAPIELGQRRHSVVAERPERKIILVTDDGEELRVALVEDGRLSEIYIE
                     RPDRKSYLGDIYQGKVENVMSGIDAAFVDFGLEKNGFLYVDEVTAPEGEKRARRISDA
                     LKNGQQVLVQVTKDPMGHKGARLSTKISLAGRYLVYVPGGSGVGVSRRLKQSERERLR
                     DISKELKPKDAGLIVRTIAEGHGLEDLRRDMRYLSRLWSRLKKKAETVKAPGLVHKEV
                     DISLEVTRDLFNDTCESLIVDDAKRHKAILSFLDKEAPELLPKVHLHTGDEPLFAAYG
                     IEEQIAKALERRVPLPSGGNLMIDHAEALTVIDVNTGRFTRGKGLEDTITKTNLEAAR
                     EVVLQLRLRDIGGIIIIDFIDMAHAKNREMVLATLEAELETDRTKTYVVELSPLGLVE
                     MTRQNTTDGARGILTRACPTCQGKARVLSEETMALSVERRVRALARKSQAKAWLVEVN
                     AAVAERLSGARLKRLEKQTGRRVFLEGGSALPVEAFRVISEGTIEHVQEQRVPVREGQ
                     EVTVELEFSLTYSPRDAVGYVDGYMVIVEGGRQHLGKRRKVRVTEIARTGARATVLKK
                     DAN"
     gene            34248..34586
                     /gene="rplU"
                     /locus_tag="VFZ86_07850"
     CDS             34248..34586
                     /gene="rplU"
                     /locus_tag="VFZ86_07850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEX5642235.1"
                     /translation="MTKPNDTYAVVRCGGQQFRVSAGDTIVVDRVDAEEGKSITLDAL
                     AVRDGKGTYDADAAKHAKVKATVSEHLLGEKIKVFTYKPKSTFKKTKGHRSRLTKLTI
                     DSITLKSTKE"
     gene            34591..34845
                     /gene="rpmA"
                     /locus_tag="VFZ86_07855"
     CDS             34591..34845
                     /gene="rpmA"
                     /locus_tag="VFZ86_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007155544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEX5642236.1"
                     /translation="MAHKKGAGTSRNGRDSESKRLGVKAFAGQVVPAGSIIVRQRGTR
                     FRPGENAGIGVDHTIFAKVTGTLVFTEKGAGRFISVVPEA"
     gene            34881..36164
                     /gene="obgE"
                     /locus_tag="VFZ86_07860"
     CDS             34881..36164
                     /gene="obgE"
                     /locus_tag="VFZ86_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEX5642237.1"
                     /translation="MLDRASICVRGGHGGNGSMSFRREKYVPKGGPDGGDGAPGGDVV
                     LVATRQLHDLSHFRHKHHFRAANGGAGRGANKRGADGPELLVQVPVGTEVRDAGGELL
                     GDLVREGQRLRVARGGEGGRGNVCFKSSTRQAPKFAERGLEGEELWLTLSLKLLADIG
                     LVGLPNAGKSSLLAALTRAHPKIAAYPFTTIDPNLGVLYLGEGTAVIADIPGLIEGAS
                     EGAGLGHRFLAHIERTALLLYVLDGGDGADAWPAALDTLRGELGAFRTELLERPAVVV
                     VNKRDLLDEAGQDAAEGFLAGLRWPAATAVASAQTGDGLKALVRTLTGVMEQARAAQS
                     EAEAAAEAAQAATVLRPAEGRLDSFTVEPVDDYFVVRGAQMERLFAKADLDNADAVAY
                     LQQVIERVGLNDALRRAGAVPGDTVVVGEAEFEFA"
     gene            36205..37311
                     /gene="proB"
                     /locus_tag="VFZ86_07865"
     CDS             36205..37311
                     /gene="proB"
                     /locus_tag="VFZ86_07865"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEX5642238.1"
                     /translation="MKTIVVKVGSSTVADDRLHLRRRLLAGLVADIAELVHGGRRVVL
                     VSSGAIACGQHVLGVTERPRQVPALQAASAVGQGRLFAHYERLFGDHELTAAQVLLTS
                     EDFNRRDSYVNARNTLRRLLSWGVVPVVNENDTTATDEIRFGDNDVLAAQVAIMLRAD
                     SLVLLTDQDGLYTMDPRRHGAAELVRRVTEPAQLAALDVGDASRRGAGGMRGKTAAAL
                     MAAAADVRTVIANGARPGVLGSVVAGEDVGTQILARATSASAFKLWLRYAKPVRGTLE
                     VDDGAARALAQGGSSLLPVGVKAVHGSFVAGDAVAIVGPLGDEVARGITTMSARDLRR
                     VRGMRSVELRALSPHLEDEVVHRDRLVLVDEEAG"
     gene            37308..38555
                     /locus_tag="VFZ86_07870"
     CDS             37308..38555
                     /locus_tag="VFZ86_07870"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEX5642239.1"
                     /translation="MKVEQTCAEAQRAARVLAGLSRARKDEVLFAVADALRRRSAEIL
                     AENDEDVRLARENRLADALVDRLLLDEDRLAEVEEAVREVAALPDPVGQLVEGWRLDN
                     GVELRRVRVPLGVIAVIYEARPNVTVDAAALCLKSGNAVILRGGSAALHTNRILAEVV
                     QGAVIETGLPREAVSYLGPDRKELAELLKQRRYVDLVVPRGGEDLKTYLLENSKVPVI
                     YAAGGNCHVYVDAAADLDKALPIIVNAKCQRPGVCNAAETLLVHQQVAAEFLPRAAEE
                     LKRRGVELVVDKAAGDAIGDPSLKRANKTHYDTEFLALKLAVRVVSSLDEAIEHIAAH
                     GTRHSEAIVTEDLAAARRFTAQVDAACVYVNVSTRFTDGSQFGLGAEMGISTQKLHVR
                     GPIALQELTCVKYELWGDGQVRS"
     gene            38559..39161
                     /gene="nadD"
                     /locus_tag="VFZ86_07875"
     CDS             38559..39161
                     /gene="nadD"
                     /locus_tag="VFZ86_07875"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEX5642240.1"
                     /translation="MAEKQPRLGILGGSFDPPHVAHLAIASEACHALGLSRVLFVPAA
                     APPHKGLGERTPAAVRLEMASLAVDDDLRFTVSGVEIERGLVYTADTLRALSARYAGH
                     DLVFIMGSDSLLQLETWHEPEELLALCSLAVAPRPGDAPEAVVAAAARWGEHRVALLD
                     LAPLGVSSSDLRRRVAARRPIRYLVPQRVEQYILETGLYR"
     gene            39158..39727
                     /gene="yqeK"
                     /locus_tag="VFZ86_07880"
     CDS             39158..39727
                     /gene="yqeK"
                     /locus_tag="VFZ86_07880"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR00488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEX5642241.1"
                     /translation="MTGIDKAAAEALIEGRLSPRRRDHAHRVAAAAVTLAGRYGASRD
                     AAEVAGLLHDYCRELPDAETLAAAARHGIPVGPVEARRPKKILHGPVAAAELEERGLD
                     PEIADAIRLHTVGAAGMTVLAKCLYLADYLEPGRDFPGIDEVRALAETSLDAAVGAAA
                     RLSLLDIIGRGRGVVPGALALYNETHAGH"
     gene            39714..40844
                     /locus_tag="VFZ86_07885"
     CDS             39714..40844
                     /locus_tag="VFZ86_07885"
                     /inference="COORDINATES: protein motif:HMM:NF024791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HEX5642242.1"
                     /translation="MPATRGYRSDRLRRRRRSRVERSQAWMLYALAAVVAFVAVLGAW
                     YISSRFGGDEPVAKKSGYLAAIQLTAPGREQPVAALLVAQDPAGGDPGVYLVPPDLLL
                     EGPNGEYVFAADAMAAGELAGDLGRVVHAPIDAVYTLPVAALGRWAGTDELQVELDAP
                     VAVEVGDRTQMVEDGDTVAVADLPAVFSDPGGDREALTALQVAAVRAVLEAAALRPAA
                     ERAGLAGVSPSPQAGPGLSEVLRRITSGNAQVELFPAGTRVAEGQFAFVPDAEAIQAG
                     ITRRAPSYDADVTVQVLNGSGRVGVGQAVAERLASLDVNLPAPLNAESFSYRQTQILA
                     GPDTLQVARDIRAILSRGVVLDGPDLPDGTIQVIVGDDFEAP"
     gene            40913..41233
                     /gene="rsfS"
                     /locus_tag="VFZ86_07890"
     CDS             40913..41233
                     /gene="rsfS"
                     /locus_tag="VFZ86_07890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEX5642243.1"
                     /translation="MVALADGKKAQDIVVLSMDEAVSYTDYFVVVSGANSRQTRAIAD
                     EIQQRLRAHRRPARVEGEREGEWILIDYIDVVVHIFTTAARDFYRLETLWGDVPRLDV
                     PTEG"
     gene            41285..41797
                     /locus_tag="VFZ86_07895"
     CDS             41285..41797
                     /locus_tag="VFZ86_07895"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEX5642244.1"
                     /translation="MMRRLTGAALVALTMLLFAGAAPALAFDRSANEAAVLRLINQTR
                     RSRGLAAVRVAGALDRAALAHSRDMIARDYFAHSSLAGAGVAARARGAGYSVSGYEQW
                     YVGEVIAWGTGTRGTPASVVKAWMNSSTHRQVILTARWRDVGIGCSRGTFQGRSGVVM
                     YTVDFGRRVR"
     gene            41889..>42401
                     /locus_tag="VFZ86_07900"
     CDS             41889..>42401
                     /locus_tag="VFZ86_07900"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEX5642245.1"
                     /translation="MFVSGRAILHLDMDAFFASVEQARRPELRGKPVIVGGERGGRGV
                     VSACSYEARAYGVHSALPIAQAERLCPHGVFLPVDMKAYLAVHRAMIKLLEQYTDLVE
                     VASIDEAYLDVTGSRRLFGPPRTIAHRVQEQVYDAHGVTCSIGIGPTKLLAKLAAGMH
                     KPAGIGELTAA"
BASE COUNT         6358 a        15137 c        15087 g         5819 t
ORIGIN      
        1 gcccgcgccc aggccgacgc ggtcgcgacc ggcgcgacgg tcgagctcac gagcgacctc
       61 aaggcggccg tgcagggagc ggacgtcatc tacaccgaca ccttcacgag catgggccag
      121 gaggccgagc acgacgtgcg tctcgcggcc ctgcgcgact accaggtgaa catggacctc
      181 gtgaagctcg ccggcccgca ggtcaaggtc atgcactgcc tgccggcgca ttggggcgag
      241 gagatcaccg aagaggtgct gtactcggag agctccgtgg tcttcgacca ggcggagaac
      301 cggctgcacg cgcagaaggc gatcatggcg ggcctcatcg cctgaggcgc gggccgcgtc
      361 ctcacgcggc cggggcgccg aggcacgaac gcggaagggg cggggcggag gccgcagtgg
      421 ccaccgcccc gccccttccg cgtgctcaga tccagctcgg gaaccacatg atttgctcga
      481 agtacttggg ggagatcggc agcccgagga cgatcggcac gaatgccacg acgatgccga
      541 tcgccagtcc cgcgacgacc atggccgtcc acgggcgata ggcgcgcatg cgagaggaca
      601 gcaggaagac cagcacggcg agggcgagcg cgaccccgac ggccacaccg acccagcccg
      661 ccgtctcgcc ggcgcgccgc gggagcgacc agaacagcca gcccgcgccg atgccgagcg
      721 gacgccagag gatcgcggcg gcgagcgccg cggccgccgc cgcgaaccac ccgcggcgcg
      781 gcagcacgcc cccggcgaag aggcacagcg ccgcggccac caggatggcc atgaacggcg
      841 ccaccggcgt catgtagtag aggaacgacg tgcgcgtcgt gaggaaccac ggcacgtaga
      901 gcgccacgac gatcgcggcc gccgggagca gcgcgtagga ccgccgcagc accgcgagca
      961 ccacggctgc ggccagcgac agcgtcgcca gccaccagag gaaggggttc ccgatggcga
     1021 tgacgccgcg gtaggtcttg gtgccctcga agtagtacca gacggggcgg tagtcgacga
     1081 tccaggtgaa cgcccgtgag gcgtaggtgt gcgtggcctg caggttcagg ttgaaggtga
     1141 tcatctgccg gtggagctcg cggaagtcgg cgagcgtgtg tccggtggcg aagtactgcg
     1201 cgtagctgag caggtagacg gcgacgggga gggcgaggag cgcggccgcg atcaccgcga
     1261 tgcgccgggc cgcggagccc tgcggcggcg ccgcgacgac ggcctccgcg tgcgggacga
     1321 cagccggcgg gccgacctcg tcgcccggcg ccgcggcgcg cgtcgcgccg tcgtcctctg
     1381 cggacgcggc gcgccgggcc gcccggcgct gcagcagcca cgcgacgacg atgatgagcg
     1441 cggcggcgaa gagcgcgagg ccgcccgacc acttggtggc gagcgccatg ccgcccgcgg
     1501 ctccggcggc gaagagccag cgcggcctcc agccgtcctg aacgtagcgg agcgtgagca
     1561 ggacgcagag cacggtccac gcggcgatga agatgtcgag ggtcgcgatg cgcgactgcg
     1621 ccaccccgag cgggtcggcg gcggcgaaga tcagggcgag cagcgcccag ggaggcgaga
     1681 ggccgagccg gcggccgagc gggtagacgc aggccagcag ggcgacgccg gcgatcacgg
     1741 ccggcagccg ccagccgaac gagcggtcgc cgaacaggag gatgccgccg gcgatggcga
     1801 acttgcccat ctccgggtgg ggccacgaca cctcgtcccc ggcggcccag cggtactgcc
     1861 cgtcggtgcc cacgcggccg tcgacgatgg ccttgccgtc cttcgcgtag tagatctcgt
     1921 cgaacatcat ctccgcgggg gtgctgagct gccacaggcg gagcgccgtc gagacgagca
     1981 ggacgagcgc caccgccagc gcgtagccgt ggcggcggtc cgggtcggcg atgcggagtg
     2041 ggcgtgcgag catctgcgtt ccgtccgagt gtcttcgcgt gtccgtttcg tgcgccgcca
     2101 gtatagtgaa tccggccgct tgtgcgcttc ggcacttggt ttgtgtgccg cttcgtgata
     2161 caaatccgcg ggtttgccgc gtcgatggct ggagccgggg gcgacgccag acctgtgacc
     2221 atcctgccgg tctaccgaga gtggaggcat cgatagatga gtgacgaacc aaggtcgacg
     2281 ctggcgagta ccacgggccc gatgggcgag cgtggcacca tcatggccac gctgcgcgac
     2341 gtgacggcca accccggccc cctcggcctg atggcgttcg gcatgaccac catcctgctg
     2401 aacatccaca acgccgggtt ctacgagatg aacaccatga tcctcgccat gggcatcttc
     2461 tacggcggga tcgcccaggt catcgccggc atcatggagt ggaagaagaa cagcaccttc
     2521 ggcatgaccg ccttcctgtc gtacggcctc ttctggctca cgctggtcgg cctcatcgtc
     2581 ttccccaagt ggatgggcgt cgacggtccc gacgacgtgg ccatgggctg gtacctgatg
     2641 gcgtggggcg tcttcaccgg cctcatgttc atcggcacgc tgcgcatcaa ccgctcgctg
     2701 cagaccgtgt tcctgagcct caccgtcctg ttcgtcctgc tggccgtcgg cgactggacg
     2761 ggcaatgcca cgatcaccaa gatcgccggc tgggagggca tcttcgtcgg cctgagcgcc
     2821 gtgtacggct ccatcgccca ggtgtggaac gagctgtacg gcaaggtcgt cctgccgctc
     2881 ggtccgtaca agaagtagct ccgcgcgttc gccgcgaccg cggctcacaa gacgccgagg
     2941 ggcgccgcca ccgcagggtg acggcgcccc tcctgcgtgt gggcgccata cgcgggtcgc
     3001 ccaccgtgcg attcttccgt ccgaggccgt ccctatcatg ctccttgaca cgaacacggg
     3061 ttcgctctag gctcgacacg aacacgtgtt cgccacagga ggaggaacgg tggaaggtct
     3121 cacgccgaga caagagcagg tactggagtt catccgcgcg agcgtgcgcg ccgacggcta
     3181 tccgcccacg gtgcgcgaga tctgtgcggc gctcgatctg tcctcgccgt ccaccgtcca
     3241 cgcgcacctg gccaacctcg agcgcctcgg gctcatcagg cgcgacccga gcaagccgcg
     3301 cgcgctcgac gtggtcggcg acgcgcgtcc gccacgtccg ctgccgctca tcggtagcgt
     3361 cgccgccggc gcgccgatca cggccgtgga caacgtggag gacctcgtcg acgtaccggg
     3421 cttcctccgc cgggacgacg acgatttcgt actccgcgtg cgcggggact ccatgacggg
     3481 cgccggcatc ttcgacgcgg atctcatcgt cgtgcacccg cagcccgacg cgcgcaacgg
     3541 tgagatcgtc gtcgccaggg tcgccggcat cgccgacacc gaggccaccg ccaagcgctt
     3601 cttccgcgag ggcgccgtcg tgcgcctcgt cgccgagaac gagaactacg agccgatcgt
     3661 cgtcaatgcc gaccaggtgg aactcgtcgg ccgagtcgtg ggggtcctgc gccagctatg
     3721 agtaccgtgc ctctcgccac agccgtccag acaagcctcg ccgacgccgt cgacgagtcc
     3781 ctcgccgccg cgctcgccgg ccgcgacgag ccctgcctgt ggtgcggagg ccactccgtg
     3841 cgcgtcctcg gagccgacct gtggagcggc gccgtcacgg tgagctgccc cacctgcggg
     3901 agcgagctca gcggcgtcgt gccgcggcgc ctgcgcgagg tgcctcgatg accgagcggt
     3961 cccgtctgta cccggtcgtc gacgaacccc agcgccgttc ggcggcccca gcctctcgct
     4021 ccatcggccg agccggccgc cgtcagcggc gccggcgtcg tgtggccggc gtcctccgct
     4081 tcgccgtgtt cctcttcctg atcttcgtgg ccgtgtgggc cggcgtccgc gtcgcgcacg
     4141 ccggcgacga tccagccatc tacagcggca cgccgtacac cgtcgcggcc ggcgacgatc
     4201 tctggaccat cgcgcaggcg aagtacggcg gcgagtcgga cctgcgcgcc gccgtgtacg
     4261 ccatccgcga cgccaacgac ctcgagacgt ccgtcctgca accgggccag gatctcacgc
     4321 ttccgtacct cggcgactag aggatcacgt accgtcgtgc gactcgcgac ccgcgcgggc
     4381 gcggcgcgag gcgaacgagg cggcccgacc ttcgccgggg ccgcgcgagc catgcggtat
     4441 acttgcgccg cgtccgggac gtggcgcagc ctggtagcgc acctgctttg ggagcagggg
     4501 gtcgcgagtt caaatctcgc cgtcccgacc aagccttacg cgggcgggag tagctcagtt
     4561 ggtagagcat cagccttcca agctgagggt cgcgggttcg aaacccgtct cccgctccat
     4621 ctttctcgtc ctgcacctcc gcgcctcgta tacttgcctt cccgcgcgcc cgtagctcag
     4681 ctggatagag caggagactt ctaatcttca ggccgctggt tcgaatccag ccgggcgcgc
     4741 cagatcgccg tgcagcgcac cttgccgacc ccgggagcgg actgtacgct gccaacggac
     4801 agcgccgccg gcgcccgcat cttcgaagga gccaggatgc cgagtagagg aagaccccga
     4861 ctcgtcgagg tttggttgtg ggacctgagc acgaagcgct cccccttcgg ccacaagacg
     4921 aacccgctcg aatacctccc cgcggatgct cccctgccgc gcgtggggga catgatccac
     4981 ttgccgccga acgtcaccgg cgacaccaag gagcaggcgt tcgggtacgg cggcacgcgt
     5041 accccgttcc gggtcgagga gtgctcgcac gtctacgcgc gtgagaagag cgagaagatc
     5101 gacgtcctcg acccctcgcc ggctcgccac gtgaagacgc tcatcttcgt ccggcgtctc
     5161 actccggaag aggtctacga tcagcggggt tgggagcggg aagcgggcgt ctgacgacgt
     5221 cggccgtcct cggcgctggc cccgcgctgt tctcgagggc ggctcctctg caccgcggcc
     5281 gcgtctccgg ctctgacaac gccaagatgt aggcgagcgg cccacgaggg tgcaaactcc
     5341 ttggtgaacg acccccggag cggatgagtc atcccccggg ggtcggtcac gtggtcgatg
     5401 gcccgccact cctaccgtta ccatcggcat cgtggcgggt agatacggtg ccgatgagcg
     5461 accgcgacga catcgatatc tccagccggg gggcgtccgc cgacgccgtg tacgcggcgg
     5521 tagccgtcgc gctggccgac cacgacaagg cgacggcggt gcgcaccgcc gtggaggcag
     5581 tgaccgcggg cgccgtgtcg atccccgagc tgtatcgcga cgtgctcgcc cggatcctgg
     5641 ccgacaccgg ggccgcgtgg cagaccggca agaccgccat ctgggaggag cacctcgcca
     5701 gctccaccgt gcggacgatc gtcgagatcc tgtacccggg cgtgctcaag gccaaggcgg
     5761 cggtcgcgcc ggcagggcgc agcgtgttgc tggcgtgccc accggacgag gggcacgacc
     5821 tcggcctgcg catggtcgcg gaccgcttcg acatggccgg ctggaccacg ttcttcctgg
     5881 ggccggacac gccggtggcc gagatcgccg acgccgccag gcggctcggc gtggacgccg
     5941 tcgtcatgag ctcgtcgacg cacttccacc gcgtcgcggt gcgccacgcc gtcgacgtcc
     6001 tcaagaagga gctccccggc acggacatct gggtgggcgg gccggcgttc gtcggcgccg
     6061 cgaccggctg gctgccgcag gaggtcgtcg atctggacgc gctgctcggc ggacccgcgc
     6121 cgggggccga cgtcgcgaac gaaggcgccg ccaccgacgc ggcggacgac ggtgcagacg
     6181 gagcggacga caccgtcacc gcgagtgggg aagagggctg aggtgctcgg ctttcggatc
     6241 gcgatccgct tcctgcgcaa gagccccgtc cagtccatcc tcatcgccgc cggcatcgcc
     6301 gtcggcatcg gcgtgcaggt ctttctgggc tcgctgatca cgagcctgca ggcgagcctg
     6361 gtcgaccaga ccatcggcaa cagcccgcag gtcgtggtga ccgccgggca ggacggcgcg
     6421 gccgtcgcct acacgccaca actccagcag gccctcgagg cgcaggtcga gatcacgaca
     6481 gtcgtcccca cgcgcgcgtt ctcggccatc tacagcagcg gccgcgagaa cgccccgctc
     6541 caggtcaccg gcggcgcgct ggacgagctg gacaccatct acgacatcac cggccggctc
     6601 gtggccggcg aggcctctct cgactcggac gaagtgatcg tcggaaagga cttcgccgac
     6661 aagtacgggc tcaggccgac cgaccagttc cccgtcacgt tgccctcggg cgagcaggcc
     6721 gggttcgtcg tgtccggggt gttcgacttc ggctccgccg ccgccaacag cgccaccgcc
     6781 ttcgtgaacg gcgatcgggc ggcagaggtg ctcggcctcg ccgcagacga gttctcggcc
     6841 gtcgacgcgc aggtggacga tgtgttctcg tccgagaagg tcgcgaccga cctgcgcggg
     6901 cagccggcgc tcgagggtct gacggtcacg gagtggcagg ccgagaacgc cgacctgctg
     6961 tccgcgctgc agagccagag ctcgtcgagc tacatgatcc agttcttcgt gctggtcgcc
     7021 gtagccctcg gcatcgcctc gacgctggcc atctccgcgg tccagaagac ccgtcagatc
     7081 ggcatcctca aggcgatggg catgaaggac cgccagtccg ggcgcatctt cctgtggcag
     7141 gccctgatcc tcggcgtgag cggcgccgcg gtgggcgtcg ccgccggcct cggcctcatc
     7201 gccctgttca cgttcctcgg gcgagactcg gagtcgctgt tcccgatcac gccgcaggtg
     7261 gggttcgtcg tcctctcgtt cgtggtcggg gtgctggtcg ccgtggtgtc ctcgttcatc
     7321 ccgtcccggc gcacgtcgaa gctcgatccc atcgaggtga tccagggtgg ctgagacgct
     7381 ggtccacgcg gaggggatag ggaagacgta cggtaccgtc gtcaagacgg tcgccctgca
     7441 cgaggtctcg ttcgagatcg cggcggggga gttcgcctcc gtcatcggcc agtcgggctc
     7501 cggcaagtcg acgctgctca acctcatcgg cctgctcgac acgcccacca cgggacgtat
     7561 cgtgctgaac gggcacgacg cgacggcggc cagccgcaag cagcgcgccg ccctgcgcaa
     7621 cgacttcatc gggttcgtgt tccagttcca ctacctcctg ccggagttca gcgtcctgga
     7681 gaacgtgctc atgcccgggc gcatcgccgg ggcggcgccg tcggacctgg tcccgcgggc
     7741 ggaggagaac ctcgcgctgc tcgggctgga ggggctggag gagaagaacg ccaacgacct
     7801 ctccggcggc cagaaacagc gcgtcgcgat cgcgcgcgcg ctcatgaacc ggccggcgct
     7861 ggtgctggcc gacgagccga ccggcaacct cgacaccgtg aacacggcgg ccgtgtaccg
     7921 cctcttccgc gagatcaacg aggagcaggg caccgcgttc atcatcgtca cgcacgaccg
     7981 cggcgtggcg cagcagaccg accgcatcct cgagatcagc gacggcgctc tcctgcagga
     8041 cgtgcgcaac gagtacctgg ccccggacgc gacgccgagc ccggagcgcg tgtgacgcag
     8101 gctgcggatg atggtatcct tctacgtcga ttgcccggcc gggtcctcat gtgcagaccc
     8161 cggccggcgg tcgaccggtg gtgaaggtag ctcagctggc agagctccgg gttgtggtcc
     8221 cggcggtcgc gggttcgaat cccgtccttc accccatctc agcgatgcac cggccggcgc
     8281 ggggcgccgg ccgtccacgt taacgatgat gcgggtgtgg cggaactggt agacgcgcta
     8341 gacttaggat ctggtgagct ctgctcgtgg aggttcaagt cctctcaccc gcaccacctg
     8401 ctcgatcatc gacccccgca cacaccatac ggcggtccgc agtgcccccg cggcgactgc
     8461 gggccgttcc ggcgacagac ggagaatcca cagtgaagac gaaggtcaac cctacaggcg
     8521 agaacgaggt cgaactcagc gtcgaggtgc ctgccgagac cgtcaggaag gcctacgacc
     8581 gcgccgtcgc caagatccgc gaggagatgc agctcccggg cttccgcaag gggcgcgtgc
     8641 cggtcaacct cgtggtccag aacgtgggcg ccgacttcat ccgcggcgag gccctcgagg
     8701 acgccatccc cgagtggggc gacgaggcgc tcaaggcggc cggcctctac gacgacgccg
     8761 tcggcacctc ggacctgcag gtcggcccgc tcgacgagac ccaggactac gcgttctcgc
     8821 tcaaggtgca gaccatgccc gtgcccgccc tcggcgagta caaggggctc gaggtcccca
     8881 agcgcgaggt gaccatcacc gaagaccaga tctccgcgca gctcgcgatg ctgcaggaga
     8941 ggctcgccag cctgcagccg gtcgaggacc gcgccgtgca gaccggcgac ttcgtgctca
     9001 tggacctcgc cggcagccgt gacggcgagc ccatcgaggg cgcccagggc aaggaccaga
     9061 tgtacgaggt cggccgcggc aacctcatcc cgggcttcga ggaggagctc gtgggtgtca
     9121 aggccggcga ggagaagagc ttcgacgtca ccttccccga ggactatcac gccgaggagc
     9181 tcgccggcca ggcggccacc ttcaccgtgg cggtcaagga gatcaaggag aaggtcaccc
     9241 ccgagctgga cgacgcgttc gccgccgacg tcagcgagtt cgagaccatc gaggagctgc
     9301 gcaacgacgt ccgcgagcgg ctgcaggcgg ccgcggacac gggcgcgcag cgcgagttcc
     9361 gcgcggccgc ggtcgagaag gccgtcgagc acgcgacgct cgccgtcccc tccagcatga
     9421 tcgaccgcga ggcgcaccgg ctctaccacc agctcgagga gcgcgtgggc gagcagggcc
     9481 tcaccatgga ggtctacctc ggcgtgctcg agaagaccga ggaggacatc gaggaggaga
     9541 tgcgcccgca ggctgagttc atcatcaagc gccagctggt gctcgaggcc atcgcgcagg
     9601 cggaggccct cgaagtgacc gacgaggaca tcaccgaggt cgtcaagcac gacgccgagg
     9661 cgctcggccg tgactacctg cagctcctcg ccgatctgga gaagtcgggc aggcgtgagg
     9721 cgctgcgcga cgaaatgcta gtggccaagg ccgccgactt cgtcgccgac gcggccgtgg
     9781 cggtgccgat gacggaggcc gaagaggccg ccgccgagga gtccgcggac gccgtggacc
     9841 ccgaagacga ctaagggttt cgagcgggga aaccacactg agagaatcaa gagtaacgaa
     9901 gagggtgagt acgtgactcc acttgttccg ttcgtcatcg agcagaccag ccgcggtgag
     9961 cggtcgttcg acatcttctc gcggctgctc aacgagcgcg tgatcttcct gggcacgccg
    10021 gtggtcgacg agatcgccaa cctcatcgtg gcgcagctga tccacctcga gagcgacgac
    10081 cccgacaagg acatcagcct gtacatcaac agcccgggcg gcagcgtcta tgccgggctc
    10141 gcgatctacg acacgatgca gttcatcaag ccggatgtca gcaccatctg cgtcggcagc
    10201 gccatgagca tgggcgcgct gctgctcgcg ggcggcgcca agggcaaacg tttcgcgctg
    10261 cccaacgccc gcgtgctcat ccaccaggtg gccggcggct acgagggtca ggccacggac
    10321 atcgagatcc acgcgaggga ggtgctcaac ctccgcaagc gtctggacga gatcatcgcc
    10381 gaccacaccg gtcaggagat cgacaaggtc cgcagcgaca cggagcgcga ctacttcatg
    10441 agcgcggccg aagctcggga atacggcatc atcgacgacg tcatcgccca ccggtaggtg
    10501 gcgcggacgc cggcctcgta cgaggaggac cgcagtggcg ggaacgaccg acggtaacga
    10561 ccagctgctc tgcagcttct gcggcaagtc gcagcgccag gtgaagaagc tcatcgccgg
    10621 ccccggcgtg tacatctgcg atgaatgcat cgacctgtgc aacgagatca tcgacgagga
    10681 gctcaccgcc ccggcgacgc tcgacctcga gcagctgccc aagccgcgtg acatctacgg
    10741 catcctgaac gactacgtcg tcggccagga cgaggccaag cgttctctgg ccgtcgccgt
    10801 gtacaaccac tacaagcgcg tccaggccgt cagcagtggc gagtccgacg tcgagctggc
    10861 caagagcaac atcctgctgc tcggccccac gggctgcggc aagaccctgc tcgcgcagac
    10921 gctggcgcgc atcctcaacg tgccgttcgc catcgccgac gcgacggcgc tcaccgaggc
    10981 cggctacgtg ggcgaggacg tcgagaacat cctgctcaag ttgatccagg ccgcggactt
    11041 cgacgtcaag cgggccgaga ccggcatcat ctacatcgac gagatcgaca aggtcgcgcg
    11101 caaggcggac aacccgtcga tcacccgtga cgtctccggt gagggcgtgc agcaggcgct
    11161 gctgaagatc ctcgagggca cggtggccag cgtgccgccg cagggcggcc gcaagcaccc
    11221 gcaccaggag ttcctcacca tcgacacgac caacgtgctg ttcatctgcg gcggcgcctt
    11281 ctccggcctg gagaagatca tcgagcgccg catgggccgg cagggcgtgg gcttcatggc
    11341 cgacgtggtc agcaaggccg acaaggaccc cggcacgatc ttcacgcact cgctccccga
    11401 ggacctgctc gatttcgggc tcatccccga gttcatcggc aggctcccgg tcgtgagcgc
    11461 cgtccacaac ctggacgaga acgacctcat gcgcatcctc acggagccca agaacgcgct
    11521 cacgcgccag taccagaagt tcttctcgct ggacggggtc gagctcgtgt tcgccgagga
    11581 gtctctcacc gccatcgcca ccaaggcgct ggcgcgcggc accggcgcgc gcggcctgcg
    11641 ctcgatcatc gaggagtcgc tgctcggcgt catgttcgac ctgccgagcc gcaccgacgt
    11701 gcgcaagtgc gtcatcacca aagagacgat cgagaagggc atcgacccga cgcttctcac
    11761 cgaggccgac aagtcgctgc cggagcgtga cgccgaatcc gcctgaacag actgagctga
    11821 acggcgaggg gcagggcggc ccacaagcgg ccctgcccct cttgccgact cgcgagctcg
    11881 tcctgttccc caggatggtc gcgccgatct tcgtcgggcg cgagcggtcc atctccgcca
    11941 tcgaggcggc cttcagcggg cagaccgcgc tcatcctctg cgcccagcgc gaagccgccg
    12001 tcgaggagcc gtcgttcgac gacctccgcc cggtcggcgt gcgcgcccgg gtcctgcagc
    12061 tgttcaagct gccggacggc agcgtcaagg ccctcgtgga gggagacgag cgcgtgcgca
    12121 cggagcggct cgtcgcgagc gacccgcact tcctcgtctc gtacgccccg gtcgagagca
    12181 ccggcgtcgg cacggcgcgc gcgcgcagcc tcgcgcgccg cgtgacggac gagttcgtcc
    12241 gctacgtgca gctgcacccg cagctcgccg acgaggcgca gttcgtggtg cagcaggcgg
    12301 ccgacgcgga cgagcttgcc gacatcgtcg ccgcgcacct gcagacggag acgggggaga
    12361 agcaggcgct gctcgagatc gtggacacgc agcagcgcct cctcgccctg ctggagagcc
    12421 tcgccgagga gaacgccgtg ctcggcatcg agcaggagat cggcctcaag gtgcagcagc
    12481 gcatcgaggg cgcgcagcgc cagatgctgc tgcacgagaa gctgcgcgtg ctgcgcgacg
    12541 agatcgagga ggccggcgag tccgcggacg aggacctggc cgggtacctg gagcgcgccg
    12601 ccgaggccgg gctctcgccg gcggcgcgca agctcgtgac gcgcgagctc accaagctcc
    12661 ggcaggcgcc tcccatgagc cccgagatcg ccgtcatccg cggcttcctc gacacggtgc
    12721 tcgagctccc ctggggcagg ctggcgccgg ccgacgtgga cgtgcccggg gtcgcccggc
    12781 acctcgagcg cacccacgac ggtctcaagg acgtcaagga ccgcatcctc gagtacctcg
    12841 ccgtgtgccg cctgcgcgcc gacgcctccc gcaagaccgg agagccggcc gacgccgccg
    12901 accagccggc caccatcctc tgcctcgccg gtccgcccgg caccggcaag agcagcgtcg
    12961 cccagagcat cgccgacgcg ctgggccggc cgttcgtgcg ggtcgccctg ggcggcgtgc
    13021 gcgacgaggc cgagatccgc gggcaccgcc gtacgtacat cggggcgatg cccgggcgca
    13081 tcgtcgacgg cctggccaaa gccggcgtgg acaaccccgt catgctcctc gacgagctcg
    13141 acaagctgga cagcgactgg cgcggcaacc cggcggcggc gctcatggag gtgctggacc
    13201 cggcgcacaa ccacgccttc cgcgacaact acctggaggc cgagtacgac ctcagccgcg
    13261 tgttcttcat cgccaccgcg aacgatgtgg acgcgatccc ggcgacgctg tacgaccgcc
    13321 tcgaggtcat ccacctgagc ggctacacgc tggccgagaa gctgtccatc gcccgccggc
    13381 acctgctgcc gcgcgtggcg cgcgacacgg ggctgcgccg cggcgacgtc aggttcggcc
    13441 gcggcgcgct gccggcgatc gtgcgcggct acacgcgcga ggcgggcgtg cgcgagctcg
    13501 agcgcgccct ccggcgcgtc gaccgcaagc tggcgcgccg ccacctggag gaaggcctcg
    13561 ccttccccgc gacggtcgcg gcggctgaac tccgcggcta cctgggtgag gctcactggc
    13621 tcgaggccga gctgccgcgg gcggcgagcg tcggggagag cctcggcctc gcctacacgt
    13681 ccgatggggg ggaggtcctc acggtcgagg ccacgctcgc ccgcggccgc ggcgagctcg
    13741 tgctcaccgg tcagctgggc gaggtcatgc aggagtcggc ttctgcggcc tggggatacc
    13801 tgctctcgca cgccgagcgc gaccggggtc tcgcgccgct ggtcggccgc tcaccgttca
    13861 tgactgcgga cgggctcgac ctctcgggcc aggacgtgcg cgtgcacgtc ccggagggcg
    13921 ccgtgcccaa ggacgggccg tcggcgggcc tggcgctcgc cgtggcgatg ctctcagcgc
    13981 tcggcggcgt gccggtcaag ccgcgggtcg ccatgaccgg cgagatcacg ctgggcggcc
    14041 gcatcctccg cgtcggcggc ctcaaggaga agctgctcgc cgcgggccgg tccggcgtcc
    14101 gcacggtcat cctcccgtcc tccaacaggg gcacggtcga ggagctttca ccggacctca
    14161 cggacaagct cgagctggtg tacgtcgcga gcttcgagga ggcgctgccg cacgtcttcg
    14221 gcaggcgcgt gtttagggaa cgggcatcta agggtcaagg caaacgagtg acaggcagta
    14281 tgggggcggc gaacatgcac gatcacgacc acgagcacga ccaccagact cccggctcca
    14341 ccgaggagcc gggcgtcgac gcgggatccg gccagctgga acagcactgc tcgcagtgcg
    14401 ggtcgctcat cggcggctcg gacgagttct gccaggtgtg cgcgatcgag tacagcgggg
    14461 gagaggcgcc ggaagaggag aagtgacccc ggcgccgtca cgcgggtccg gagagggccg
    14521 cctctctctg cgaggggcgg ccttcttcgc gtctcgcacc aggcgccgac gggccgcagc
    14581 acccgtcgcg cgtgacggcg cgcgcgccgg gcgctcggca ggcgccgctc cggcccggta
    14641 tactctcgac tcggccgcgg cggcacgtcc gccccgcggc tcgcggaccc gtggaccggg
    14701 tccgcccgtg tgaaccagcc gccgcggagc ccttcggcgc cgcggcccgg cgcggatcga
    14761 gacagaggcc catgaccgac ccgagcagcg gcaccgacta cctgcagcag acgacccgct
    14821 tcgagcccgc cgccgtcgag gcgcggtgga tgaagcgctg gctcgacgag catctcttcc
    14881 acgccgagcc cgacccggac aggccggcgt actccatcgt catcccgccg ccgaacatca
    14941 ccggcgtgct gcacatgggc cacgccctca acgacaccgt ccaggacgtg ctcacgcgct
    15001 atcaccgcat gctcggcgag aacgcctgct ggctggtggg caccgaccac gccggcatcg
    15061 cgacgcagaa cgtggtcgag aagatgctgc gcaaggaggg cctcaccaag gaggacctcg
    15121 ggcgcgagga gttcgtggcg cgggtgtggg agtggcgccg cgagtacggc ggcaccatca
    15181 tcgagcagct caagcggctc ggctcgtcgt gcgactacga gcgcgagcgc ttcaccttcg
    15241 acgagggcta cgtgcgcgcc gtgacgcgcg tgttcgtcgc cctctacgac aagggctaca
    15301 tccacaagga cagctacctg gtcaactggt gcccgcgctg cggcacggcg atcagcgacc
    15361 tcgaggtggc ctacagggac atgcccggga cgctcaacca catccgctac gagatcgccg
    15421 gcggcggcag cgtcaccatc gccacgacgc gcccggagac gatgctgggc gacaccgccg
    15481 tggcggtgca ccccgacgac gagcggtaca cggccgtcgt cggcaagacc gccgtcctgc
    15541 cgctgctcgg gcgcgagctg aggatcgtcg ccgacgagcg cgtcgacccg tcgttcggca
    15601 ccggcgccct caagatcacg ccggcccacg acctcaccga cttcgacatc ggccgcacgc
    15661 acgggctgga gaccgtccag gccatcggcc aggacggccg catcaccgcg gccggcggcc
    15721 cgtacgccgg gctcgaggtc gaggaggccg cggcgcgcgt gaccgcggat ctgcgcgcgc
    15781 tcggcgccct ggtcaaggtc gaggactacc cgcacagcgt cgccacctgc gaccgctgcg
    15841 gtacgcccat cgagccgctc atcagcgagc agtggttcat ggacatggac gagctcaaga
    15901 agccggccac ggacgtggtc cgcgacggca ccgtcacgtt cacgcccgag cgctggggcc
    15961 gcgtctacat cgactggatg gacaacctgc ggccgtggtg catcagccgg cagctctggt
    16021 ggggacacca gctgccggtg tggtactgcc cggacggcca cctcacggtg gccgagaccg
    16081 agccgcaggc ctgcgccacg tgcggctcca ccgagctcac ccgcgagacc gacgtgctcg
    16141 acacgtggtt cagctcggcg ctctggccgt tcgccacctt cggctggccg gagcagaccg
    16201 ccgacctggc ctacttctat ccgacgaacg tgctgagcac ggggcgcgac atcatcttcc
    16261 tctgggtcgc gcgcatgatc atgatgggcc tcgagttcat ggacgagatc ccgttcgacg
    16321 acgtctacat ccactccgtg atccaggcgc cggacggccg ccgcatgagc aagagcctcg
    16381 ggaccggcat cgacccgctc gacgtgatcg acgagcacgg cgccgacgcc acgcgcttcg
    16441 gcttgctgct catgagcagc acccaggacg tgcgcttcag cgacgagaag atcgccatgg
    16501 gccgcggctt cgccaacaag ctctggaacg cggcccgctt cgtgctgctc ggcacccagg
    16561 ggtacgcggc cgagcgcagc gacgtcgccg aggtggaccg ctggatcacg agccgcttcc
    16621 agcggtgtct cgccgcggtc gaggaggcgg tggccgtcta cgacttcacc gcggccgtgg
    16681 acgcgctcta ccacttcgtg tgggacgagt tctgcgactg gtacgtcgag ctgatcaagg
    16741 tcccgctgta cggggaggac gaggcgaaga aggcccaggt cgcgggccac gcccgctgga
    16801 tgctcgacca gatcgtgcgc ctgctgcacc ccttcatgcc gttcgtcacc gaggagatcg
    16861 ccgagcagta cggcgcggcc ccgctgctca ggcagcagca cccgcgccgt gacgaggagc
    16921 aactggccgc ggacgacgag aaggccatcg gagagctcca ggccgcggtc gacgcgctgc
    16981 ggcgcttccg cgccgaggcc gagatcccgc cgggcaaggt gctcgcggcc gtgttcgccg
    17041 gcgacgccgc cgcggccgag cgctacgagc accacgcggg cgtctttcgc gccatggccc
    17101 gcacgaccct cgccttcgcg ggtgagccgg agacggacgc gacggtcgtc gtcgtccccg
    17161 gcgggcggct cttcgtggcg gcggcgatcg acaaggcgga ggagatcgcc cgtctcgagc
    17221 agcagctcgc cagggcgcag gccgaggtcg agcgcggtga ggccaagctc ggcaacgaga
    17281 agttcgtcgg ccgcgcgcct gaggccgtgg tgaccaagga gcgcgagaag ctggcgagcc
    17341 acgtcgccga gcgcgacggc ctggcggcga gactcgcgca gctgcgcggc gggtgagtgc
    17401 gggcgcaccg tgagcgcaga cgccggcaac cttgagtggg acgcctacct cgagtcgctg
    17461 gccgccttcg gcatgcgccc cgggctggag cgcgtgaccg agctcctcga gcgtctcggc
    17521 cggccccagg acgcctacag cgtcatccac atcgtcggca ccaacggcaa gtcgtccacg
    17581 acgcggtacg ccgaggcgct gttgcgcgcc cacgggcgac gctcgggcgc gtatctctcg
    17641 ccgcacatct ccggctggac cgagcgcgtc atcgtcgacg ggcggcccgt cgaggagccc
    17701 gtgttcgccg cggccgtgga acgcgtgcgc gaggagatac cggccctgcc ccgcggcctc
    17761 ggtgagacga cgcagttcga ggtgctcacc gtcgccgccc tgctcgcgtt cgcgcggagc
    17821 ggggtggagg ccgttgccct ggaggccggg ctgggcgggc gcctcgacgc cacgaacgtg
    17881 gtcaaggcgc ccgtcgtggt gctgaccaac atcgcgctcg agcacaccga ggtgctcggc
    17941 ggcacgcggg ccctgatctt cgccgagaag gcggcggtca tcaagggcgg cgatgtcgtg
    18001 ttcggcgacc tcgacggcct cgagggcgag gcgcgccggg tctgctccgc caccggcgcg
    18061 acgccgcact tcctgcgcga gtccgcgtcg cagggcgacc tggccgtcgt gggctcgccc
    18121 gaggacttca cggtgacgtt cgcgccgggg gaccggccgg agcgctgggc cggcctgcgc
    18181 gtgccgacgc cggccctgta ccaggtgcgg aacgcggcgc tcgcgctcgc cgcggcgcgc
    18241 ctgctgctcg gcggcctgga cgaggccgcc gcccggcggg cgctggccgg caccgccgtc
    18301 ccggggcgcc tgcaggtggc cggagagcgc ccgctggtgc ttgccgacgg agcgcacaat
    18361 cccgacggcg tgcgtgtcct cgcgcagagc ctcgccgccg tgcgcatgcc gcggccggcc
    18421 gtcggcgtgc tcgccatcat gcgcgacaag gactaccgcg gcatgctcga cggcttcctg
    18481 ccgctgctcg accacgtcgt ctgcacgcag gcgaccgaac cgcgcagcct caccgccgat
    18541 gagctcgcga ccgccgtgca cgcgtccgcc cgcgctcgcg gggggcggcc gccggcggtc
    18601 gccaccatcc gcgacgcgca cgccgctctc gcccgggccc gtgagctcgc cggggaggcc
    18661 ggcagcgtgc tcatcggcgg ctcgctctac ctgctggagg acctgagcga cgtgctcgcc
    18721 gccggcctct gaccccggct tgccctgcgg gggttcggat atactggcgc aaacccgtgg
    18781 agaggtgcat ggcacagcag agggcccatc ccgtcgtacg ctttctcgtt gcgttgctca
    18841 tcgtggtcgc cgtcgcggcg gtcgccttct tcgtggggta cctcatcggg atgcgtctgg
    18901 gcgctggtgt cgcgctcccc gtcaccttct aggcgcgcca gccaggcagc accgctcctg
    18961 caggagggac catgaccgac cccgcttctc gcgaccagct catccttgtc accggcgcca
    19021 ccgggtacgt cggcggccgc ctcgtgccgc gcctgctcgc cgccggccgc cgcgtgcgcg
    19081 tgctcgcccg cgacccccgg cgactcgagg gccgcgactg ggcggagcgg gtcgatatcg
    19141 tcgccggcga cgtcctgcgt ccggagtcgc tcgcagcggc gttggacggc gtcggcgtcg
    19201 cctactacct cgtgcactcg atggcgaagg gcgaggggtt ccacgagcgc gacctcgtgg
    19261 cggcgagagc cttcgcgagc gccgccctgg ccgcgggcgt cagtcgcctc gtctatctcg
    19321 gcgggctcgg cgatcccgcc gccgacctct cccggcacct ccgctcgcgt caggagaccg
    19381 gcgaggcgct gcgcgaggcc ggcgtaccgg tcaccgagtt ccgcgccgcc gtcgtcgtcg
    19441 gctccgggag catctccttc gagatgatcc gctacctcac ggaacggttg ccggtcatgg
    19501 tgtgcccgca gtgggtgtac acgcgcgtgc agccgatcgc cgtcgacgac ctgctgcgct
    19561 acctcgtcgc cgcgctcgac gtaccggaga gcaccggtcg cgtggtcgag gtgggcggca
    19621 ccgatgtgct cacctacggg gagatgatgc tcgggtacgc gcgcgcccgg ggcctgcggc
    19681 ggcgcctgca gccggtgccg gtcctcacgc cgaagctgtc gtcgtactgg gtgcacctgg
    19741 tcacaccgat cccctcgacg atggcccgcc cgctcatcga aggtctccgc aacgagacga
    19801 tcgtgcgcga acagggcgcc cacgacctgt tccccgcgat ccacccggtg ggctatgacg
    19861 cggccgtgcg cgccgccgtg gcgagcctcg acacgggaga ggtcgagacg agctgggcgg
    19921 acgctctcgt cacgagcggg ggggacgtgc agccgcgggt cctgaccacg aaggagggca
    19981 agctcatcga acgtcgccag gcgctggtcg cggcgacgcc cgaggagacc ttcgccgtcc
    20041 tccagaccat cggcggccgc cgcggctacc gtgcctggga ctgggcgtgg gaactgcggg
    20101 gcgccgccga ccggctggtc gggggcgtgg gcctgcgccg cggccgccgc gacccggacg
    20161 aggtgcgcgt cggcgacgcg ctcgacttct ggcgcgtcga ggcggtcgag gcgggccgcc
    20221 tgctgcgtct gcgcgcggag atgaaggtgc ccggcgcggc ctggctgcag ttcgagacgc
    20281 tgccgcacga cggcggcacc ctgctcgtgc agaccgcgta cttcgcgccc cgcggcgtgc
    20341 ccgggctcgc gtactggtac gcgctgctgc ctgtccacag ccggatattc tcgggtatga
    20401 ttgcggcgct ggccgccgag gccggccggc cggccgcgcc ggcggggggc tgagcgcacc
    20461 cgcctgcgag cacggccgcc atcacgtgga ggcgccgggg gcgtcctcga cggacttctc
    20521 cagaggagga agggtgcctt tctggaccat cgttgcggcg aacagcacca ccacgacgag
    20581 cgacagcagc aacccgttcc agtccgtgct ggacttcttc gagtcgccca cctggcagtt
    20641 cctgaccatg ctcttcttct tcttcctggt ggtgctctgg atcgcctgcg cctactgggt
    20701 cttcaaagac gcgcgccggc gcatcgacga caagatcgtg ctcaccgtcg ccgtcctcac
    20761 cggcatcgtc ttcggaccca tcgggctcat cgtgtacgcc atcgtgcgcc cgcccgagac
    20821 gctcgccgac cgccgggagc acgagctcga ggtgcagatg atggaacagc gcatcggcga
    20881 ggaggagcgc tgctccttct gcaagacccc ggtgcgcgag gactacctgg tgtgccccac
    20941 gtgcgcccgg cgcctgcgca cctcgtgccc gtcctgccgc aagccgatcg acccgcggtg
    21001 gcgggtctgc ccgtactgcg aggcggacgt gcacgccatg ccgcccgtct tcgaccgcgt
    21061 ctgacgccgg cgcgcgagcg cgggcagcag cgcctcacag tttcatccgt tcaccccttc
    21121 ggaggactcc caatggaacg cacgttcatc atggtcaagc ccaacggcgt cacccgcggt
    21181 ctcgtcggcg aggtcgtcgc ccgcttcgag cggcgcgggt tcgcgctgcg cggcatgaag
    21241 gccctgcgca tcgaccgcgc cctggccgag aagcactacg ccgagcacgt gggcaagccc
    21301 ttcttcgaga gcctggtggc cttcatcacc tccggccccg tggtcgccat ggtgtgggaa
    21361 gggcgcgagg ccatcagcgt cgcgcgcgcc atgatgggcg tcaccgactc cgcccaggcc
    21421 gcgcccggca ccatccgcgg cgactactcc ctgagcaagg aagagaacgt ggtacacggc
    21481 tcggacggcc ccgagagcgc ggcccgcgag atcgccctgt tcttcggcga gtccgacctt
    21541 gtctgacccg gtcccgcagc acttctggga gcgtgtgctg ccggacaggc cgggcgagcc
    21601 ctccgtcggc ttcggcggcc tcgtgctggc gtcgcgctcg ccgcagcgca agctgctcct
    21661 ggagcagctc ggccttccat tccgtgtcgc cgtcagcgag cacgacgagg gcgtgagcga
    21721 cggtgacccg gcgcacaccg tgcagcagaa cgcccgtggc aaggccgagg acgtcatggc
    21781 ccgcgagacc gtgcgtgcgg gcgagctggt cctcggcgtc gacaccgtcg tcgtgtgcga
    21841 gggggagatc ctcggcaagg cgcgggacga ggccgaggca gccgccttcg tgcgccgcct
    21901 cgcggcgcgg cgccacgagg tgtacagcgg cgtgtacctg acctcccgca agctcgcgct
    21961 ggcgacccac gtggtcaccg gcgtcacctt ccgcagcctc accgacgcgg atctcgatgc
    22021 gtacatcgcc tcgggtgagt ggcgcgaccg ggccggcgcg tacgccatcc agggcatcgg
    22081 atccggcctc gtgcggcacg tcgacggcga ctacttcaac gtcgtcgggc tgccgatcgc
    22141 ggagctcatg cggctgctcg cgagcttcgg cgtcgcgccg ttctcctggc tgcccgggca
    22201 ggtctgaggg cgacttctcg ggacggggcg agggtacctc cggcgggcgg cgaagtagcc
    22261 cccgcaggcg ggtcatggac gcagcgtccc tccaggggcg ccggcggaac ccctctgcgc
    22321 cgcccacgag ccggcgcggc acgcccccgg gcgcgcggtg gccgcgccca gaggcagcgt
    22381 atacaatcgt cgttcgagat tcgcttcgaa aggagctcgc catcagcttc ctcgggttcg
    22441 taacgggctt tggcgcccgt gacatggcgg tcgacctcgg caccgccaac acgctcgtct
    22501 atctccgcgg caagggcatc gtcctcagcg agccgtcggt ggtcgccatc gaccaccgct
    22561 ccggtgaggt ccacgccgtc ggcgcggagg ccaagcgcat gctgggtcgc acgcccgcca
    22621 cgatcagtgc ggtgcggccc ctcaaggacg gcgtcatcgc cgacttcgac gtcaccgagc
    22681 agatgctccg ccacttcatc cgcaaggtca tgcagaaccg ctgggcccat ccacgcgtcg
    22741 tgatctgcgt gcccagcggc gtcaccgggg tcgagaaacg cgccgtcgag gaggccacgt
    22801 actccgccgg cgcccgctgg gcgaccctca tcgaagagcc catggccgcc gccatcggcg
    22861 ccggcctgcc ggtcgccgag cccacgggca gcatgatcgt ggacatcggc ggcggcacca
    22921 gcgaggtcgc ggtcatctcc ctcggcggca tcgtcgtcag ccagtccatc cgcgtcggcg
    22981 gcgacgagct ggacgaggcg gtcatcagct acgtgaagaa ggagtacaaa ctcctcatcg
    23041 gcagccagac cgccgaggag atcaagctcg agatcggctc ggcctacccg ctcccccagg
    23101 aggagcaggc cgagatccgc gggcgcgacc tcgtcacggg gcttcccaag acggtggtgc
    23161 tctcgtccga ggaggtgcgc accgcgctcg aggagcccac gcaggcgatc gtcgacgcga
    23221 tcaaggcgac gctcgacaag accccgccgg agctcgccag cgacatcatg gaccggggca
    23281 tcatgctggc cggcggcggg tcgctgctgc agggcctcgc cgagcgcctg cgcgacgaga
    23341 cccagatgcc ggtgcacgtc gccgaatcgc cgctgacctg cgtggccgtg ggctcgggca
    23401 agtacctcga ggagttcgat gtcctggtca agagcaagcg caactcccgg ccgcggggcc
    23461 ggtagtccca cccctcgctg acccgtgaat cgccggcacg tcctccggcg gcggatcgtc
    23521 gtggcagtcc tgctgctgct cgccgtcgcg ctgctcaccg cgtacttccg cgagtcggac
    23581 agcggcgtgg cgcacagcgt gcagggcggg gtcctcacgg tcgtctcgcc gctgcagagc
    23641 ggcaccgcca ccgcgaccaa gcccttccgc gacgcctgga actgggtcgg cgacctgttc
    23701 ggcgccaagt ccgagaacac ggccctcaag aaagaggtgc aggacctgcg cgcgctgctc
    23761 gccgaagagc tcacgacgca ggcggagaac gagcagctgc gcgcgctcct ggacttccag
    23821 gaggaccgcg tgtaccccaa agacgtgcag ctggtgacgg cgcgcgtcgt cgcgcgctcc
    23881 acgagcgcct ggtactccac ggcgaccatc agcgccggca gcagcgacgg ggtggacgtg
    23941 tacgacacgg tcgtcaacgg gcagggcctc gtggggcgcg tcaccaaggt gaacgcgaac
    24001 gcggcgcagg tgctgctcgt cactgaccag cagggcttcg tcgacgccat ggtcgagccg
    24061 ggaggcgcgc agggcctcac cagcggcagc gtgaccggcg acgtcacgct cgagtacgtg
    24121 gacaagaacg agaaggtcaa ggtcggtcag tacgtcgtga cgtcgggcag aaacggatcg
    24181 gtgttcgtgc gcggtatccc cataggccag gtcgagagcg tcgtccagca ggacgtcgag
    24241 ctgtaccagt ccatcgccat caagccgttc gtcgacttcc acaagctgga catcgtccag
    24301 gtgatcacgc gttgagcgtc atccggcccg tcgccgagaa gaccgagcgc gcctggccgg
    24361 cgtggggcga ggtggcgcgg ctcgccgtcg tgctgctgct cgccgtgctg ctgcagacgg
    24421 ccgtggcgcc gcacctgcgc ctcctcggcg cctacccgga cttcgccctc atcgccgtgg
    24481 tctgcgtggc gctcgtcaag ggctcggaga ccggcgccgt gttcgggttc ctcacgggga
    24541 tgctcacggc gatcgcgctc atggagccct tcggcctgag cgcgttcgtc ttcgtgctcg
    24601 tcggctactt cgccggcagg tacgccgaga ccgcggacct gccggccggc ctcgcgccgc
    24661 tgctcaccgt cttcgccgcg accctggtcg ccaacgtgct gttcgccatg tcccagttcc
    24721 tcctcgcgcg cgaggtgccc ctgggcttct tcctcgggcg ggtgctcttc ccgtcgctcg
    24781 tgttcaacac gctgctcgcc gcgccggtct atctgctggt gcgggtgtgg ctgagagggg
    24841 caggtgacct gcgtgccgct gctcggacgt gaccccgacc gccgcgaccg gcccatccgg
    24901 accggccgcc gcgacaagcc catcatccag ctcccctccg tcgccctgcg cgtggcggtc
    24961 atcctcggcc tggccgcggt gctgttcggc gtcgtcttct tccgcctctg gttcctgcag
    25021 atcctgagcg gccaggagtt cgtcgcccag gccaacgaca accgtctgag ctcggtcaag
    25081 gtggtcgcgc cgcgcggcaa catcgtcgac cgcaagggcg aggtgctcgt ggagaaccgc
    25141 gcggggaccg ctgtcggcat ccgcctcatg gccgtgccgc aggggcagct cgacgcggag
    25201 atagagcgcc tggcgggggt gctcaagatg cgcccgggca agatccgcaa ggagatcatg
    25261 gactacctgc ggccgagcac gatggagcag gacgaggacg gcacgtggac cagcttcctc
    25321 acgtggcgca aggtgctgga cggcgatatc accggcctcg acctgatcgt ggtgaaggag
    25381 gacgtgagca agggcgtgcg ctcgtacctt aaggagcaca cgctggactt ccccggcgtt
    25441 gaggtccgcg accagtacct gcgctcctat ccgcagggcg acatggcggc gcagctgctg
    25501 ggccatctcg gcgagatcga tgccgatgag ctcaaggatc agcacttcaa ggggtacgcg
    25561 gccggcgacg tggtcggcaa ggacggtctg gaggacacct acgacgcgtg gctgcgcggc
    25621 cgtgacgggg tcgcccgcat cgaggtggac gcctacggcc ggcccaagca gacggagccc
    25681 gtgggtggcc gcatggcgga gccgggccac accctcgtca ccaccctcga tgtcgacgtg
    25741 caggcggccg ccgaggaggc gctgcggacg ggcatctcgc tggcgcacaa ggacggcaag
    25801 tacgcggcca acggcggcgc ggccgtcgtc ctcgacgtca ggaacggcga gatcctcggc
    25861 atggcgagct acccgaccta cgaccccggc gtctgggtcg gggggatcag cacgaaggac
    25921 tacgagcagc tgaccaggaa gagcgccaac aacccgatga tcaaccgtcc catctcggag
    25981 accaaggccg tcggctcgac gttcaaggcg gtgaccgcgg tagcggcgct cgaggagggt
    26041 cacatctcgt ccggcaccgg ctacttctgc ccgggctcct acagctcgat gtacgacaac
    26101 gcggacccgc cgcagaagtt caagtgctgg tcggacggcg gccacgggac cctcgacctg
    26161 gtgggggcga tcacccagtc ctgcgacgtg tacttctaca acgtcggcga ccgcttctac
    26221 ggacgccagg gcagcgaggc gctcgaggac tgggccaaac gcttcggcat gggcaaggtc
    26281 accggcatcg acatccccgg cgagtccgcg ggtcgcgtgc ccacgccggg ctggaagaag
    26341 agctacttcg agacggagat cgacaagatc tggaagccgg gagactccgt caacctctcc
    26401 gtcggtcagg gcgacctcga ggcgagcccg ctgcagctgg ccgtgagcta cgccgccgtc
    26461 gccaacggcg gcaaggtcgt cacgccgcac ctcgggctgg aggtggtcga cgccgccggg
    26521 cagaccgtgc gcacgctgga gcccgcgccc ccgcgcaagg tcgacatctc gcagagcacg
    26581 ctcgacgtgg tgcggcgtgg cctctacgag gccgcgcaca acggcgccgg cacctcggcg
    26641 gccgtcttcc agaactacga cgtggcggtg tcgggcaaga cgggcacggc cgaagtctgg
    26701 gacgacacct ccaagcacta cgtcgactac gcctggtacg cgagctacgc gccctcggag
    26761 gatcccagat acgcggtcgt cgtcctgatc gagaagggcg gccacggctc caccgccgcc
    26821 gctccggcca cgcgtctcat ctacgacgcc ctgttcggca tcgacggcgg cgccttcacc
    26881 ggcgcgccgc ggggtgactg atgcacgaag ccgggcgtag ccaagcgaga gctattgagc
    26941 ggggcgcggc ccgcaggctc agcgggtcac ggaccaagcg ctgacgtggt cgactggcgc
    27001 gcgtacttcc ggcacctcga ctacctgctg atcctggcga ctgccgggct gatcgtgtac
    27061 ggcgtgacga tgatctactt cgcgacccgg cacgacatcg acggcgcgcc cctctactac
    27121 gtgcgccagc agctgatcgc ggtggcgatg gggctcgtgg cggcgatcgt cgcctcgctg
    27181 ctcgactacg agatctaccg ccgcttccag tggctgctgt acgccgccgc ggtcctcatc
    27241 gtggtcgccg tgctgccgct cggcgagaac gtcaacggcg cgacgcgctg gatcgacctc
    27301 ggcttcacgc gcttccagcc gtcggcggcg gcgatgctcc tgctcgcgct gagcatcggc
    27361 gcgttcctcg cggaccggat ggaactgctg ggatccaagc ggatcaccgt catcgtgctg
    27421 gtcctggccg ccgtgccggc gttcttcgtc taccaggagc cggacttcgg ctcggcctcg
    27481 gtgatcatgg tgctcgcgct ggccatgctc ttcttctacg gcacgccgtg gacacacttc
    27541 gccgtgctca ccgccacggg ggtcacggcg ttcgtggtgg cgctcaaggt gctgccgctg
    27601 gccggcgtcg agctcatcaa gcagtatcag gtggaccgtc tcttcgtgtt catcaacccc
    27661 ggacacgact ctgccgggac cgggtacaac gtcatccagt cgatgatcgc cgtcggctcg
    27721 ggcgcgctca cggggcgcgg cgaccaggcc acccagacca cgctcgactt cctgccggag
    27781 caccacaccg acttcatctt cgccgtgatc agtgagcgca acggtttcgt gggcgccagc
    27841 atcctcctgg tgctctatgc tctgttcatc tggcgcgcgc tgcgcatcgc ggtgatggcg
    27901 cgcgacatgt acgggagcat cctcgccggc ggcatcggcg tcatggtcct cttccaggtg
    27961 ttcgtgaaca tcgggatgac gattggtatc atgccggtga ccggcatccc gctgccgttc
    28021 atcagctacg gcggcacggc gatgatcgcc ttcctgatct tcgtcgggct gcttcaagcg
    28081 atccacctgc gggcgacgat cacaccagaa tcgagggggc ggcttggcca gtagcttgag
    28141 catctcgaag atcctgaagg ccgtcaccga cgcgcgcggc agcgtggatg cctcgcgcgt
    28201 ctgcgtcgtc ggcgtggggg acgacctcgc cagggtcgtc ggcgtgctct cggcgggggc
    28261 ccgcgacgag agcggcgggg tgtccgccaa ggtcgacgcc ctctcaccgg ccgacttccc
    28321 gcgcgagcag cgcctcgccg cgcgctggga catcgtcgtc ctcgtggccg cgggctgccc
    28381 ggccgccgag gcgtcacccg tggtccacgc cgcgcgccgc ggaggctgca aggtcatcgg
    28441 cctggtcgag gagccgggcg aggccgcctg ggcgcgcgag gccgggctct tcgacgacga
    28501 ggtcgcgcgc ggcctcggct acgacgcggc cggcaagccg acgctcgagc gcaagatcgt
    28561 cgaggccgca ggcgacggcg ccgccgccct cgccgcggcg ctgccgtccg tcaggcgcac
    28621 gtactgcgac catgtcgtga tcaccaacgc cgcccagaac ggggtcatcg gcgtggtggt
    28681 catcatcccc ggcgccgaca tgccggcgat gaccgccaac cagatccgca tggtgctcaa
    28741 gatcgcgcac gcgtatgggg aggagatggg catcgaccgt gccgtcgaga tcctctccat
    28801 cgtgggcaca gggttcgtcc tccgcacgct ggcgcgtcag gcgctcgact tcgtgccggg
    28861 attcggctgg gccgtcaagg gcgccgtcgg gttctcgggc acggtcgcgc tgggccaggc
    28921 cgccatcgcg tacttcgagg ccggggcgcc gctccggctc tcgcggatgc aaaggatcaa
    28981 cagacaggtt gaaaagctca cgtcccggct tcccgggccg ctccaacgac tggcctccgg
    29041 atgaagtagc gaggcttctg gcgcgcgtca cgcgccccgg gcgctacgtg gggggtgagt
    29101 tcaacacccg cctcaagccc ggggcccgtc cccggatcgt gctgtcctac ccggacgtgt
    29161 acgagatcgg ggtctcgaac cccgcgctgc agatcctcta cagtcacctg aacgacgcca
    29221 cgccggcctc tgccgagcgg gcctactgcc cgtggccgga catggccgac ctcatgcgcg
    29281 ccgccggcgt cccgctgtgg acgctggaga cgttcgcgcc ggtcggcgag tgcgacctct
    29341 ggggcttcac gctgccgcac gagctctgct acgcgaacgt cctcgagatg ctcgacctcg
    29401 cgggagtgcc gctgcgcgcc gccgagcggg atgggggaga ccccatcgtc ctcggcggcg
    29461 gaccggcggt cgccaacccg tggccgctcg cgccgttctt cgacgccttc ttcatcggcg
    29521 aggcggagga ccacctggac gccatcgcgg aggcgatgag cgcggacggc cgcgagaggc
    29581 gcctgcgggc gctcgcggcc gtgcccggcg tgtgggcgcc cggccgcagc gacgggccgg
    29641 ccgagcgcca ggtgttcatg ggattctcgg ccgccatgcc cgtgctgcgt ccggtcgtgc
    29701 cggtgctcga ggcggtgcac gaccgcgtcg tcgtggaggt catgcgcggc tgcaccgccg
    29761 gctgccgctt ctgccaggcg ggcatgtggt accggccggt ccgggagcgg ccggtcgacc
    29821 tgatcgtcac ggccgccgac cggctcctgg acgagaccgg gtgcgacgag gtctcgctgc
    29881 tctcgctcag ctcgtgcgat tacggcggag tcgccgaggc gctgacccgc atccgggaac
    29941 tgcgccccgg cctgcgcgtc tcgctgccgt cgctgcgcat cgactccgcg gcgcagagcc
    30001 tcgcgagctt cgccgccggc cagcgcggct cgctgacgct ggcgcccgaa gcggggacgc
    30061 aggagctgcg cgacgcgatc aacaaaggcg tggacgaggc gcagttcgag gaggccgtgc
    30121 gggcgacgtt cgcgggcggg ttcaccgggc tcaagctcta cttcatgatc gggttgcccg
    30181 gggagaccga cgacgacgtg ctcggcatcg ccgggatggc cgggacggcc gcgcggctgg
    30241 cgagagagat cggccgcggc cgcgcacggc tctcggtcgc cgtctcctcg tacgtgccca
    30301 aggcgcacac gccgttcgag ctcgagccgt tcgccggcga gtccgtcctg cgccgccgcc
    30361 agcagctggt gcgcgaggcg gcgccgagga acgtccgcgt cgcgttccac gacgtcgcgg
    30421 cgtcggtggt cgaggcgacg ctggcgcgcg gcgggccggg gaccgaccgc ctggtcgagg
    30481 aggcctggcg gcgcggggcg cggttcgacg gctggagcga gcacttcgac gtgcgccggt
    30541 gggaggacgc ggcggcgagc accggcgtcg ccctcggcga gcggaccgtg gagcgcgcgt
    30601 tctggcgaga cgccgtggac gcgcgcctgc acgaggactt cttcgcggac gagctcgcgc
    30661 gcagccggca gggcgacctg accgaggact gccgcgacgg ggcctgcgcc gcctgcggcg
    30721 tctgcgccgg cgaggtcggg atggacctcc tcgcatgagc tggtggctca tcgccttcgc
    30781 gcgccgtggt acggcgcgat acctctcgca cctggacacg gcgcgaacgg tgcagcgcac
    30841 cttcgcgcgc gccggggtgc ccatcgccct ctcgcacggc atgcggccca agccgcgcct
    30901 gtcgctgccg cttccgctgc cggtgggtgc ggccggagac gaggagctcg cggtcgtcga
    30961 ggtgcccgag ggcgtggacg cgtccgcggt cacgctgaag gcgctgcgcg cggcgagccc
    31021 gcccgggttc gagccgctga gcatcgtgga cgcgggtgag cagcgtccgc acccgcagcc
    31081 gagggcggcg gagtacgcct gcctgctcga cggggacgca ggcacgatcg tcgcggccgt
    31141 cgaacgctac aacgacgcag aagacgcgat ccgcgagcgc gtgtccccca agggcacgcg
    31201 ccgcctggat ctgaagcact acgccgggga cgtcggcgcg acgccggtgg agggtggagc
    31261 ccgtctaggg ttcaccatcc accaccgccg tgacggcgcc gcccggccac aggaactgat
    31321 cgacctgatc gccgagtggg cggaggtcga gccggcgatg cgagggctgc aacggctgcg
    31381 catcgcctgg gacgatcggc cggcgggcct cagggccgcc ggcggaagga gttgaccacg
    31441 tgagcacagc gaagaagggc acgcccggcg acgagccggc ggcgacgccg accggccaga
    31501 acggcgccgg cgaaggtcag gagacgacgg gcgccgccgg cgaggccgct ccgcgccgcc
    31561 ggcgcggcaa ccgcggcggg cgccggcaca agcggccggc gacggctgcg ggcgacgccg
    31621 gtcaggcgca ggcgacggct gcggccgacg cgagtcaggc gcagacgccg gcgcagacgc
    31681 cgcgagacgg ctacgccgcc gcgaccgacg cacttgcgtc gctgccgacg gcgccgctga
    31741 tccccgtcgg cgccgcggag gcagccgcgc gcgacaaggt cgccgccgat gaggctgcgc
    31801 gaggcgacgc ctcgcccggc ggcgagcggg cgccggccgg cgaagcgcgc gcaccgcgcg
    31861 gcagaagccg cggcggccgc gggcgcgcca ggcccagcgc cgccgcgacg gagacgcccg
    31921 tcgtggcgcc tgtcgccgcg cccgagccgg agaaggcggc ggacgagccg gccgccggcg
    31981 ccgccgcgga cgcgacgccc gccggcgagg aagaagagac gcacgccaag cgccgccgcc
    32041 gcggcggccg cggcggtcgc cgccggcgca agacggccga gatcgagcac gaggaggcga
    32101 cgcgcgaggc cgaggaggcc gccggcgagc cgctggccag cggcgccctc gcggaggagg
    32161 cgccggcacc tgcacccgcc gcggaagtgg caggccagcc ggcaggcgag cgagccgacg
    32221 gcgcccaggc cggtgaggcc ggcgccaagc gccgtcgcgg cgcgcgcggc ggacgccgca
    32281 cgcgcggcaa ggcggaggct gccgcggcgg agcccgccga ggcgtcggag gcggcggagc
    32341 ccgcagcaga gcctgggtcc gtggcggggc cggcgaaagc cccggccgcc gagcccaaga
    32401 cgcggcggcc tcgcgaccgc cggcgcccaa gggcgatcgt ccgccaggac gagggcagcg
    32461 gtctcgacac gatcgcgccg atcgagctcg gccagcggcg ccattcggtc gtggccgagc
    32521 gtccggagcg caagatcatc ctcgtcaccg acgacggcga ggaactgcgc gtggcgctgg
    32581 tcgaggacgg gaggctctcc gagatctaca tcgagcgtcc ggaccgcaag tcgtacctcg
    32641 gcgacatcta ccagggcaag gtcgagaacg tcatgagcgg catcgacgcg gccttcgtcg
    32701 acttcggcct ggagaagaac ggcttcctgt atgtggacga ggtgaccgcg ccggagggcg
    32761 agaagcgcgc ccggcgcatc agcgatgcgc tcaagaacgg gcagcaggtg ctggtgcagg
    32821 tgaccaagga cccgatgggg cacaagggcg cccgcctgag caccaagatc tctctggccg
    32881 ggcgctacct cgtgtacgtg cccggcggga gcggcgtcgg cgtgtcgcgg cgtctcaagc
    32941 agagcgagcg cgagcgcctg cgcgacatct cgaaggagct caagcccaag gacgccggcc
    33001 tgatcgtgcg caccatcgcc gaaggccacg ggctcgagga cctgaggcgc gacatgcgct
    33061 acctctcgcg cctgtggtcc cggctcaaga agaaggccga gacggtcaag gcgccgggcc
    33121 tggtgcacaa ggaggtcgac atctccctgg aggtgacgcg cgacctcttc aacgacacct
    33181 gcgagagcct catcgtcgac gacgccaagc ggcacaaggc gatcctgtcg ttcctcgaca
    33241 aggaggcgcc ggagctcctc cccaaggtgc acctgcacac gggggacgag cccctcttcg
    33301 ccgcctacgg catcgaggag cagatcgcca aggcgctcga gcggcgcgtg ccgctgccga
    33361 gcggcggcaa cctgatgatc gaccacgccg aggcgctcac ggtcatcgac gtcaacaccg
    33421 gccgcttcac gcgcggcaag ggcctcgagg acaccatcac caagaccaac ctcgaggcgg
    33481 cccgcgaggt ggtgctgcag ctgcgcctgc gcgacatcgg cggcatcatc atcatcgact
    33541 tcatcgacat ggcgcacgcc aagaaccgcg agatggtgct ggcgacgctc gaggccgagc
    33601 tcgagaccga ccgcacgaag acgtacgtgg tcgagctctc gccgctcggc ctcgtcgaga
    33661 tgacgcggca gaacacgacc gacggcgcgc gcggcatcct gacgcgcgcc tgtcccacct
    33721 gtcagggcaa ggcgcgcgtg ctcagcgagg agacgatggc gctgagcgtc gagcgccgcg
    33781 tgcgtgccct ggcgcgcaag tcacaggcca aggcgtggct cgtcgaggtc aacgccgccg
    33841 tcgccgagcg cctgagcggc gcgcgcctca agcgcctgga gaagcagacc gggcgccgcg
    33901 tgttcctcga gggcggctcc gcgctgccgg tcgaggcgtt ccgcgtgatc tccgagggca
    33961 ccatcgagca cgtgcaggag cagcgcgtgc ccgtgcgcga gggccaggag gtcaccgtgg
    34021 agctggagtt cagcctcacg tacagccccc gcgacgccgt cggctacgtg gacgggtaca
    34081 tggtcatcgt ggagggaggg cgccagcatc tcggcaagcg acgcaaggtg cgcgtcaccg
    34141 agatcgcgcg cacgggcgcg cgggccaccg tgctcaagaa ggacgcgaat tgacccgggc
    34201 ggccggtttg gctatcattt cctgccgttg tcttgaggga gaaaccaatg acgaagccta
    34261 acgacaccta cgcggtcgtc cgctgcggtg gccagcagtt cagagtgagc gccggcgaca
    34321 ccatcgtcgt cgaccgcgtc gacgccgagg agggcaagag catcacgctc gacgcgctcg
    34381 cggtgcgcga cggcaagggt acctacgacg ccgatgcggc caagcacgcg aaggtcaagg
    34441 cgacggtcag cgagcacctc ctcggcgaga agatcaaggt cttcacctac aagccgaaga
    34501 gcacgttcaa gaagacgaag ggtcatcgca gtcgtctcac caagttgacg atcgactcga
    34561 tcaccctcaa gagcaccaag gagtaagggc atggcccaca aaaagggcgc cgggaccagc
    34621 cgcaacggcc gcgactccga gagcaagcgc ctcggcgtca aggcgttcgc cggccaggtc
    34681 gtcccggccg gatcgatcat cgtccgccag cgcggcaccc gcttccgtcc cggcgagaac
    34741 gccggcatcg gcgtcgacca caccatcttc gccaaggtga cgggcacgct cgtgttcacc
    34801 gagaagggcg ccggccgctt catctcggtc gtccccgagg cctgagcaga ggccggcgtc
    34861 cgccggcttc ggtctgcacc atgctcgaca gggccagcat ctgcgtgcgc ggaggtcacg
    34921 gcggcaacgg ctccatgagc ttccgccgcg agaagtacgt gcccaagggc gggcccgacg
    34981 gaggtgacgg cgcgcccggc ggcgacgtcg tgctggtcgc gacgcgccag ctccacgacc
    35041 tctcgcactt ccgtcacaag caccacttcc gcgccgccaa cggcggcgcc ggccgcggcg
    35101 ccaacaagcg cggcgccgac ggccccgaac tgctcgtgca ggtcccggtc gggaccgagg
    35161 tgcgcgacgc cggcggcgag ctcctcggcg acctcgtgcg cgagggccag cgcctgcgcg
    35221 tcgcgcgcgg cggcgagggc ggccgcggca acgtctgctt caagagctcc acccgccagg
    35281 cgccgaagtt cgcggagcgg gggctcgagg gcgaggagct ctggctcacg ctctcgctca
    35341 agctgctcgc cgacatcggc ctggtggggc tccccaacgc gggcaagtcg tcgctgctcg
    35401 cggcgctgac gcgggcacac cccaagatcg cggcgtaccc gttcacgacc atcgacccga
    35461 acctcggcgt gctgtacctc ggcgagggga cggcggtgat cgccgacatc cccgggctca
    35521 tcgagggcgc cagcgagggc gcgggactcg ggcaccgctt tctcgcgcac atcgagcgca
    35581 cggcgctgct gctgtacgtg ctcgacggcg gggacggcgc cgacgcgtgg ccggcggcgc
    35641 tcgacacgct ccggggcgag ctcggcgcgt tccgcaccga gctcctcgag cggcccgccg
    35701 tcgtggtcgt caacaagcgg gacctgctgg acgaggccgg gcaggacgcc gccgaggggt
    35761 tcctcgccgg gctccgctgg cccgcggcga cggccgtcgc ctccgcgcag accggcgacg
    35821 gcctcaaggc gctcgtgcgc acgctcaccg gcgtcatgga gcaggcgcgc gcggcccagt
    35881 ccgaggccga ggccgccgcg gaggccgccc aggcggcgac cgtcctccgg ccggccgagg
    35941 gacggctcga ttccttcacc gttgagcccg tggacgacta cttcgtcgtg cgcggcgccc
    36001 agatggagcg gctcttcgcg aaggcggacc tggacaacgc ggacgccgtc gcctacctgc
    36061 agcaggtgat cgagcgcgtc ggtctcaacg acgccctgcg gcgcgccggc gcggtgccgg
    36121 gcgacacggt cgtcgtcggc gaggcggagt tcgagttcgc gtgaccgctc ccaagggcgc
    36181 cccgcccgcg ctacaatccc gcgcgtgaag accatcgtcg tcaaggtcgg ctccagcacg
    36241 gtcgccgacg accgcctcca cctgcgccgc cgccttctcg ccggtctcgt cgccgacatc
    36301 gccgagctcg tccacggggg tcgtcgcgtg gtgctggtct ccagcggcgc catcgcctgc
    36361 gggcagcacg tgctcggcgt caccgagcgg ccgcgccagg taccggcgct gcaggcggcc
    36421 tccgcggtcg gccagggtcg cctgttcgcc cactacgagc gcctgttcgg cgaccacgag
    36481 ctcaccgccg cccaggtgct gctcaccagc gaggacttca accgccgcga cagttacgtc
    36541 aacgcgcgca acaccctgcg ccggctgttg tcgtggggcg tcgtgccggt ggtcaacgag
    36601 aacgacacga cggccaccga cgagatccgc ttcggcgaca acgacgtgct cgcggcgcag
    36661 gtggcgatca tgctgcgcgc ggactccctc gtgctgctca ccgaccagga cgggctgtac
    36721 accatggatc cgcgcaggca cggcgccgcc gagctcgtgc gccgggtcac cgagccggcg
    36781 cagctcgccg ccctcgatgt cggcgacgcc tcgcggcgcg gcgccggcgg catgcgcggc
    36841 aagaccgcgg ccgcgctcat ggccgcggcg gccgacgtgc gcacggtgat cgccaacggc
    36901 gcacgtccgg gcgtcctcgg gtccgtcgtg gcgggcgagg atgtcggcac ccagatcctc
    36961 gcccgggcca ccagcgcctc ggccttcaag ctgtggctgc gctacgccaa acccgtgcgt
    37021 ggtacgcttg aagtggacga cggcgcggcg cgtgctcttg cgcagggggg gagcagcctg
    37081 cttcccgtcg gcgtcaaggc ggtgcacggg tcgttcgtcg ccggcgacgc cgtcgccatc
    37141 gtgggaccac tcggcgacga ggtcgcccgc ggcatcacca cgatgagcgc tcgcgacctg
    37201 cgtcgcgtcc gcggcatgcg cagcgtcgag ctgcgggccc tgagcccgca cctggaagat
    37261 gaagtcgtgc accgggaccg tctggtcctg gtcgacgagg aggcaggatg aaggtcgaac
    37321 agacgtgcgc ggaggcgcag cgggcggcgc gcgtgctggc gggcctgtcc cgggcacgta
    37381 aggacgaggt cctgttcgcc gtcgccgacg ccttgcggcg gcgctccgcg gagatcctcg
    37441 ccgagaacga cgaagacgtg cgcctggcgc gcgagaaccg cctggccgac gcgctggtgg
    37501 accgcctgct cctggacgag gaccggctcg ccgaagtcga ggaggccgtg cgtgaggtcg
    37561 ccgcgctgcc ggaccctgtc ggccagctgg tcgaggggtg gcggctggac aacggcgtgg
    37621 agctgcgccg cgtacgcgtg cccctcggcg tcatcgcggt gatctacgag gcgcggccca
    37681 acgtcaccgt ggacgcggcc gcgctctgcc tcaaatcggg caacgccgtc atcctgcgcg
    37741 gcggcagcgc cgccctgcac accaaccgca tcctcgccga ggtcgtgcag ggcgccgtca
    37801 tcgagacggg cctgccgcgt gaggcggtct cgtacctcgg ccccgatcgc aaggagctcg
    37861 ccgagcttct caagcagcgc aggtacgtcg acctcgtggt cccgcgcggc ggcgaggacc
    37921 tcaagaccta tctgctggag aacagcaagg tgccggtcat ctacgccgcc gggggcaact
    37981 gccacgtcta cgtcgacgcc gccgccgatc tcgacaaggc cctgcccatc atcgtcaacg
    38041 ccaagtgcca gcgccccggc gtgtgcaacg ccgcggagac gctgctcgtg caccagcagg
    38101 tggcggcgga gttcctgccg cgcgccgccg aggagctcaa gcggcgcggc gtcgagctgg
    38161 tcgtggacaa ggcggcaggc gacgccatcg gcgatccgtc gctcaagcgc gccaacaaga
    38221 cccactacga cacggagttc ctggcgctca agctcgccgt ccgtgtcgtg agctcactgg
    38281 acgaggccat cgagcacatc gccgcccacg gcacgcgcca ctccgaggcc atcgtcacgg
    38341 aagacctggc ggcggcgcgg cggttcaccg cccaggtcga cgcggcctgt gtctacgtca
    38401 acgtctccac ccggttcacc gacgggagcc agttcggcct cggcgctgag atgggcatct
    38461 ccacgcagaa gctgcacgtc cggggtccga tcgcgctgca ggagctcacc tgcgtcaagt
    38521 acgagctgtg gggcgacggg caggtgagga gctagccggt ggccgagaag cagcctcgcc
    38581 tgggcatcct gggcggcagc ttcgacccgc cgcacgtcgc gcacctcgcc atcgcctccg
    38641 aggcgtgtca cgccctcggt ctctcccgcg tgctcttcgt gcccgcggcc gccccgcctc
    38701 acaagggcct cggcgagcgg acgccggcgg cggtccggct ggagatggcc tcgctggcgg
    38761 tcgacgacga cctccgcttc accgtgtcgg gcgtcgagat cgagcgtggc ctggtctaca
    38821 ccgccgacac gctgcgcgcc ctgagcgcgc gttacgccgg ccacgacctg gtgttcatca
    38881 tgggatcgga ctccctgctg cagctcgaga cctggcacga gccggaggag ctccttgcgc
    38941 tgtgctcgct cgcggtggcg ccgcggccgg gcgacgcgcc ggaggcggtc gtcgccgcgg
    39001 cggcgcgctg gggcgagcac cgggtggcgc tgctcgacct ggcgccgctc ggcgtctcct
    39061 cgtccgacct tcgccgccgc gtcgccgcgc gccgcccgat ccgctatctg gtgccgcagc
    39121 gcgtcgagca gtacatcctg gagactgggc tgtaccggtg accgggatcg acaaggcggc
    39181 cgccgaggcg ctcatcgagg gccggctgtc gcccaggcga cgggaccacg cgcatcgcgt
    39241 cgcggcggcg gcggtgacgc tcgccgggcg ctatggagcc tcgcgggacg cggccgaggt
    39301 cgccggcctg ctccacgact actgccggga gctccccgat gcggagacgc tggccgcggc
    39361 cgcgcgccac ggcatcccgg tcgggcccgt ggaagcgcgg cggccgaaga agatcctcca
    39421 cgggcccgtg gccgccgccg aactggagga gcgcggcctc gacccggaga tcgcggacgc
    39481 catccgcctg cacaccgtcg gcgcggccgg catgaccgtg ctcgcgaagt gcctgtacct
    39541 cgccgactac ctcgagcccg ggagggactt ccccggcatc gacgaggtgc gcgccctggc
    39601 cgagacgtcg ctcgacgccg ccgtcggcgc cgccgcccgc ctcagcctcc tggacatcat
    39661 cggccgcggt cgcggggtgg tccccggggc gctcgcgttg tacaacgaga cccatgccgg
    39721 ccactagggg ataccgcagc gaccgcctgc gccgccggcg acgctcgcgg gtggaacgct
    39781 cgcaggcctg gatgctctac gcgctggcgg ccgtggtggc cttcgtcgcc gtcctcggcg
    39841 cctggtacat ctccagccgg ttcggcgggg acgagcccgt cgcgaagaag agcggctacc
    39901 ttgcggcgat ccagctcacg gcgccgggcc gggagcagcc ggtcgccgcg ctgctggtcg
    39961 cgcaggaccc cgccggcggc gaccccggcg tgtacctcgt gccgccggac ctgctgctgg
    40021 aggggcccaa cggcgagtac gtcttcgccg ccgacgcgat ggccgccggc gagctggcgg
    40081 gcgacctggg ccgcgtggtg cacgcgccga tcgacgccgt gtacacgctg ccggtggcgg
    40141 cgctcgggcg ctgggccggc accgatgagc tccaggtcga gctcgacgcg ccggtggcgg
    40201 tggaggtcgg cgaccgcacg cagatggtcg aggacgggga caccgtcgcc gtcgccgacc
    40261 tgcccgcggt cttctccgac cccggcggcg accgcgaggc gctcacggcg ctgcaggtcg
    40321 cggccgtgcg cgccgtcctc gaggccgccg ccctccggcc ggcggccgaa cgtgccgggc
    40381 tcgccggggt gagcccctcc ccgcaggcgg ggccggggct ctccgaggtg ctgcggcgca
    40441 tcacctcggg gaacgcgcag gtcgagctgt tcccggccgg gacgcgggtg gcggaagggc
    40501 agttcgcgtt cgtccccgac gccgaggcga tccaggcagg catcacccgg cgcgctccct
    40561 cgtacgacgc cgacgtgacc gtccaggtgc tcaacgggag cggcagggtg ggagtgggcc
    40621 aggccgtggc ggagcgtctc gccagcctcg acgtcaatct gccggcgccg ctcaacgcgg
    40681 agagcttctc gtaccggcag acccagatcc tcgccggccc cgacacgctc caggtcgctc
    40741 gcgacatccg tgctatactc tcgcgcggcg tcgtcctcga cgggcccgat ctgcccgacg
    40801 ggacgatcca ggtcatcgtc ggcgacgact tcgaggcccc gtaatccagc acaaaggatc
    40861 agccgtagcc gcccgcccgg aacccacaag ctccgagagc ctggcccgcg acatcgtcgc
    40921 cctggccgac ggcaagaagg cgcaggacat cgtcgtcctc agcatggacg aggcggtgtc
    40981 gtacaccgac tacttcgtcg tcgtcagcgg cgccaactcc cgccagacga gggccatcgc
    41041 cgacgagatc cagcagcgac tccgtgccca ccgccgcccc gctcgtgtgg agggggagcg
    41101 cgagggagag tggatcctga tcgactacat cgacgtcgtc gtgcacatct tcacgacggc
    41161 ggctcgcgac ttctatcggc tggagaccct gtggggcgac gtcccgcggc tcgacgtgcc
    41221 gacggagggg tgagcgtccg cagccagaaa agggcccgag agcccggcca tggggaggtc
    41281 caccatgatg cgcagactca cgggcgcggc actcgtcgcg ctgaccatgt tgctgttcgc
    41341 cggggcggcc ccggcgctcg cgttcgaccg ttccgcgaac gaggcggcgg tgctcaggct
    41401 catcaaccag acgcgccgga gccgggggct cgccgccgtc cgcgtggccg gcgcgctcga
    41461 ccgggcggcc ctggcgcact cgcgtgacat gatcgcccgg gactacttcg cgcactcgtc
    41521 gctggccggc gccggagtgg ccgcacgggc gcggggcgcc ggctactcgg tgagcgggta
    41581 cgagcagtgg tacgtgggtg aggtgatcgc ctggggcacg ggcacgcgcg gcaccccggc
    41641 gagcgtcgtc aaggcgtgga tgaactcgag cacccaccgc caggtcatcc tgaccgcgcg
    41701 ctggcgtgac gtgggcatcg ggtgctcgcg cggcaccttc cagggccgct ccggcgtggt
    41761 gatgtacacg gtcgacttcg ggcggcgcgt caggtagccg ccggccggcg cggcgccttg
    41821 ggcgcggtcg ccggccgttg accccggacg ttgtcttgcc gtccccaccg ggggtaagat
    41881 acgaacgtat gttcgtatca ggcagagcca tcctccacct cgacatggac gccttcttcg
    41941 cctccgtgga gcaggcgcgc cgccccgagc tgcgcggcaa gccggtcatc gtcggcggcg
    42001 agcgcggggg gcggggagtg gtctcggcct gctcctacga ggcgcgcgcc tacggcgtgc
    42061 acagcgcgct gcccatcgcg caggccgagc ggctctgccc gcacggggtg ttcctgccgg
    42121 tcgacatgaa ggcctacctc gccgtccacc gggcgatgat caagctgctg gagcagtaca
    42181 ccgacctcgt cgaggtggcg tccatcgacg aggcgtacct ggacgtcacc ggcagccggc
    42241 ggctgttcgg cccgccgcgc accatcgccc accgcgtcca ggagcaggtc tacgacgcgc
    42301 acggcgtcac ctgctccatc ggcatcggcc ccaccaagct gctggccaag ctggccgccg
    42361 gcatgcacaa gccggccggc atcggcgagc tcaccgccgc c
//
LOCUS       DASUMX010000160        29874 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_912820, whole genome shotgun sequence.
ACCESSION   DASUMX010000160 DASUMX010000000
VERSION     DASUMX010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 29874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29874
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_912820"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            353..1846
                     /locus_tag="VFZ86_07905"
     CDS             353..1846
                     /locus_tag="VFZ86_07905"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5642246.1"
                     /translation="MAWEVQAEVPGGFAGEAGLVDGLRAGSGEAFKALHEVYAAPIYN
                     LALRIVDDPEEAQDVAQEVLLKVFRGLPGKETELNLAAWLYRVTVNAAFDHVRASKRR
                     PVTVMDEQAPEAPAAVDEYERAELARRVETTLRELPKRQQLALVLRDVHGLSVGETAS
                     VLGLTKGSADVLLSRARAGFRRTFLAGAGSLRGRCDRADKALAAGVGRGEPVPSKLLE
                     HSRTCPDCRRAVKLWGVAPVGLGLLLPQVALPAKLSLGATLAAAEAAGIAVPAGLAAA
                     SAAGASAAGSAVAGSAAAGAAAGSGAAATAGGTGLLAALGSAAGVKVATLAAVATAAV
                     SMTGLAARHADAPAGVRDPQAGLRQQAGEPGRDGRAGRREAGLPERELQRAPRRNAGD
                     GPGGGGIRAEETGARGTGSNVSGVSTAGAGDGAGSTGSTRDAGRSSGGDTQASGAGGN
                     TQNTVAGSGAGSGQAPGGGGDAPSGDAETGGTGGSGGGSAGGTVSRE"
     gene            complement(1833..2843)
                     /locus_tag="VFZ86_07910"
     CDS             complement(1833..2843)
                     /locus_tag="VFZ86_07910"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX5642247.1"
                     /translation="MQAAEHSAGGEMRPLVIANPAAGRGRTGRSLASLIQAVRGAVGD
                     LDVAETVRRGDATALAAAAARDRRPLVVGLGGDGTLDEIVNGLLADGPRDAALLPALG
                     IVGTGTGGDFGRSLGIPHELGAYLSALRDGADRAVDIGWARFPDLEGRPVERHWINVL
                     SAGVGGLVDRYTAGAPAFLDGRLAYAQATLRAIVMCRRVKLVCGFVSPDGERRELLVD
                     AQAVAICNGRTFGGGMNIAPMAKLDDGLLEVIVVQTKSRWRLVRRLTTVYAGTHLLEP
                     GVDHFACREVELRPASPPAGRRPRAGYFPLDVDGDPLGDVPVTAGVARAALRVRASVT
                     PG"
     gene            3006..3626
                     /locus_tag="VFZ86_07915"
     CDS             3006..3626
                     /locus_tag="VFZ86_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642248.1"
                     /translation="MSASITTSKRYLALAVALLALAVGFFAFTASAQAQTLQLAGGYT
                     QLTTDPGTTKALVGARILPLPILPSWVIPTTSNGQLALRYRFYITGGQIDGQTLGGEI
                     LHSGGLKFANLKNGKSLAVKRFTIDTNAKQLTAYIPALGARAAILDLDLSGVQVSAGK
                     VYTKVGPVPAKLTPAAAGALNASLGTNLFTPGLALGTAYVYARFAN"
     gene            3828..5282
                     /locus_tag="VFZ86_07920"
     CDS             3828..5282
                     /locus_tag="VFZ86_07920"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HEX5642249.1"
                     /translation="MTSMRRPPIEPIERAFAFTYHSVEQLDRLKQATLDILETVGVRF
                     ESARALDLLAEAGARVDRVRQVAKLPPALVLEAMATAPRRFVLGARDAECDLSVGDGR
                     TYCTTDGCGVETIDFHTRERRRSTKADLADVSRLQDALSSIAFWWPTVSAGDHGETAQ
                     LHELDAGWNNTVKHLQGMVNGEREARYAVEMATVIAGSAEELRRRPVMSDLIGTISPL
                     VVDKDAIEAALVFAAAGVPVTWVTMPTLGTTAPATKAGAYALGSAELVAATVVLQLAS
                     PGAPVLHSTMQSWADPRSGDFVSFPLDGRTRALGTELAHHWGVPAESAACGTDSPVPG
                     TWQAGVEEALDLVQAAQEGSDLLPSIGLVNVYTLFFPEHLVLGDDVYRRARYAVMDID
                     MDDESVALDAIAEVGPGGHFLGNRHTRRHMRDAVVPALTHRLGPDGAPRDPVAVARER
                     AEELWREYRPEPLEEDKAAELARILAAADAELRG"
     gene            5376..5906
                     /locus_tag="VFZ86_07925"
     CDS             5376..5906
                     /locus_tag="VFZ86_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642250.1"
                     /translation="MDENVNATQGDPAGGEVRHGLIGMVRADEVDLRQSASWIATARG
                     GADLHQSASIALVSGGDTTMKMAAAVMVPTLGDAHLEKSGAQWVVAAGDVSIEKGGCA
                     AAVAPTVKVDHGAVGVALGWHVDVGEGGRVMFGPLAGAAFGIAFGIGAGLVMALGAGY
                     AAKKALGRVPQLPWRA"
     gene            5903..6658
                     /locus_tag="VFZ86_07930"
     CDS             5903..6658
                     /locus_tag="VFZ86_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEX5642251.1"
                     /translation="MTAAAADGRDAAARAEAVLAELASLGSEEARAGMARYGIETGDA
                     FGVSISQLRPLAKGLGRDHDLALALWATGNHEARLLASMVDDPASVSAEQMDVWAAAF
                     DSWDVCDQVTSNLFDKTPHAWDKVQEWSAARDQWVKRAAFAMAAALAVQDKQAADGPF
                     LEILELCRREAGDDRNFVKKAVSWALRNIGKRNPRLHAAAIATAEAILAEAEERAAAS
                     RKDPAARSGRWVARDALRELRSEKTLERVRRRG"
     gene            6753..9428
                     /locus_tag="VFZ86_07935"
     CDS             6753..9428
                     /locus_tag="VFZ86_07935"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing SpoIIE family protein
                     phosphatase"
                     /protein_id="HEX5642252.1"
                     /translation="MSLSRHEALGGQMPVGATSTSFDAIRRRHPRVLRTVTWLRSHPV
                     LCGIAGTMGIAAVFFIDMSYPGVLVYGLYYVPLFFLALAVNWRAVLFAVALCIALSAF
                     VYFWEESLDTDRGFILLYMAMIGAALVMLSYLIKRLSTTTEYAELRAQLSEAGADILG
                     GGRSRDDLDELLEYALERLGEQLDATNGVLLLLDGGEWRGWAGFGLGVDARQVAARHA
                     DVPLAAQALRADAALSRDFTNGDPSPLAPLAAHVRLERVLVIPMRSLEREVGVLVYNR
                     PQEAGEFSDEQVGLAEGLARYVGVTVDNVRLMVELNSRRRDLELVRDSSLDFAQSIDM
                     DEVLEAVVRRLVGVLDMRACDIFEVDMDGGVMRNLVTYEDGAFDTGERVGREYPIDYF
                     ATSAMAIRSRRPVIVTSVDDPRLNYAERDYLRHLGRNTELVIPLRIRENVLALVKLLD
                     DTEGRDFSNEEVELARSICRFAALAIDKARLYDQQRATAERRDRLARRLQRLQSFAVD
                     LNQRLDRADLQEVLDEVTRAAVDLLRARSAAVVSGSGDFVAVRSLAVAGSATPGGLAA
                     AEADLIERCTPGMAPGGEDFAGAGGVAGEGGHDGRLLLAPLAGEAAPQPSALVVADKE
                     QGEFDGEDRLLLATLAAQLNASMHNAIAYQREHVIAETFQQALLMEPPAIPGIEVGVH
                     YRAATDSARIGGDFYDLVSLGPGKLMVIVGDVCGKSLAAAAQSAVVRYMLRAYAAEGS
                     PGEALSRLNSAVITQTPNQPFVTLVVAYVDVARHMFEYAVAGHPRPIVLAGLEEFPMP
                     GEGNVPVGIFRGVTYPTNRAVLPDDSCVVLYTDGITEARRDRVLFGEERLHEAVRANR
                     PLDAQALADALLETVKEYSGGVLADDCAVVAVKLP"
     gene            complement(9415..10572)
                     /locus_tag="VFZ86_07940"
     CDS             complement(9415..10572)
                     /locus_tag="VFZ86_07940"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX5642253.1"
                     /translation="MNGIRVGRIFGIEVAIHPSWFIVLAFFAFSLATGFFPRAYPGWS
                     PETTWAIAVLATLLLFASVLAHEFGHSLVARSQGIPVRNITLFILGGVAQLTREPDSP
                     GREAWMAIAGPLVSVAIGVATLGAAFALPGPEQLVALLTYLGITNLALVAFNLLPGFP
                     LDGGRVLRALLWRITRDRVRATTWAAAAGQVFAWGFIALGVAQILFSGLGGIWLILVG
                     WMLIQAARATARQTLIEERLAGVPAARLMTTPGSWVSPYMTLDWVARDRLGDWDTRCL
                     PVAAEDPDAEYGGLMCSRDLRRAARERYDRDRVRDVMTPAPEMPAVAPDTGAVEVLRL
                     LRERESDRAVVVDPGGRLLGFIDVDAFTRFLSVARPRRRGVPPPATPPTAA"
     gene            complement(10675..11958)
                     /locus_tag="VFZ86_07945"
     CDS             complement(10675..11958)
                     /locus_tag="VFZ86_07945"
                     /inference="COORDINATES: protein motif:HMM:NF021387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HEX5642254.1"
                     /translation="MNAPAWAWCGARRLPIARADRFEPPAAAAPLADLDLAVRAALAA
                     PVGAARLRELARGARSVVVTIPDASRPCPSEPVLLALLDELAAAGVRDDAVAVAIGCG
                     LHATTGEAERARLAGPQVVRRVEVFDAQGIETETADLGETSLGAPARIARRVAEADLV
                     VTAGVVEPHLYAGFSGGVKGVAIGCAGRETIAWTHRPAFISRPGVRVARLRENPFQAT
                     LREVAARTPLGWGVNLVMEEGDAVAVRAGDPASVQEALARDAAPGWLRTTAEQYDVVV
                     AGVHAPKSDGLYQASRAATYLGLAARPALADGGLLVLCADLPLGAGDGPGERNFLEVL
                     AAAASPAELLERGLREPLGPGGQRAYVVARVLERHRLAVVGADDARFLEPLAHLGVAA
                     FASVDAALAAEDERLARRASVLVVADAMTTLVTAG"
     gene            complement(11955..12200)
                     /locus_tag="VFZ86_07950"
     CDS             complement(11955..12200)
                     /locus_tag="VFZ86_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642255.1"
                     /translation="MSENPVAYGAYLAAWAFFAYGPQLVICMWLAWSACRRTRGDLLD
                     WLVRGFLWSLLPFVGVVVMWWTWRRASREAAGAAPAA"
     gene            complement(12197..14275)
                     /locus_tag="VFZ86_07955"
     CDS             complement(12197..14275)
                     /locus_tag="VFZ86_07955"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HEX5642256.1"
                     /translation="MPPTRVKEEGQSKDTDKLVRRLSLVALLLSRRGQPVSAGEIRER
                     VEGYWGMTDEAFKRRFYEDRFELGELGIGIRSEDDPLADASTEVYSLPADAYYLEPVQ
                     LDGDEFAALAACLAVLEHRFAYSQPLRLALLSLAQGRPELLTEAETPPLRVEPEAHAA
                     AAALPRLQAAVVERKTVTFSYYAITRDQKTERVVDPYGLQLVSGEWYLIGHCHLREAV
                     RTFRVSRIESKVKYHTRRPHDFSPPADFDLAQYRDRPPWQLAKASGTARIRVSAAMAW
                     WVEAHWAHCGTVTRLDDGGIVYETPYADARPLLGWILGLADDAELLEPDDLRGRLRGQ
                     LESLATLLDAPPPERPAPGKRTAPGPKPDKRRRTGDDEMKVEVDRFTRLTALAAYLLR
                     RCDEDEATLEVAEVRAALGVTDKELRADVKLLNVVNFGGDGCLLWAEFKGTKRLYVSC
                     EAAGPALARPARLSPLQADTLLLAIELVGRHLPTSSGAALRSAAVKLRAARGGTPALG
                     AGDLLLAPDDVLTAVNRAIVERRLLSIQYWTEGTDRVTDRVVEPYLLVHSRGEWYYVC
                     WCRTAGGTRVFRVATTKSVKLEDETFAPRADVELGLYLREGIPPSGSYAPKEAAVWYS
                     PAVARWIAERQPVEELPGGACVASQPYVNDRWLAAHLLRFSDQARPLEPPEAVEGLRA
                     AVRRLLDRYR"
     gene            complement(14398..15861)
                     /locus_tag="VFZ86_07960"
     CDS             complement(14398..15861)
                     /locus_tag="VFZ86_07960"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HEX5642257.1"
                     /translation="MAYADKCYVGKRVKLDLLDAAEIRQIHEATLDVIESVGVKFHSQ
                     RALDILEEHGAAVDRETTVAKIPAAVVERAMSTVPHEYVLGARNPEYDLPLDGEHVYI
                     SSDGCGVFARDPETGEVRSSRKQDLADCAKVVQALDNVSATSAVVSAQDCPPETRVLH
                     EFDACVRNSEKHSIVVSIKEDWEARSLLKMAEALAGGKEELKRRPMFTTIICTVSPLH
                     QERYGMDLALTLAEAGIPVSFYPMPILGATGPITIAGSAVVNNAEFLSGAVLVQLAHP
                     GAPVMHGGGPTAMYMNSGAYASNSPEALLLRACQGHMADFYGVPAWYGAGATTAKEPG
                     IQSAYENALAMFMAYANGADVTFGTGLLDGSRILCLENMVVDDEIIGMVKRILTGIEV
                     TGETLAVELIKEMGFNGNYLFANHTRAHVRELWQARLGETGNYDGWKNAGAKSTTEKA
                     QEKVAEILAAPAPEFPADLGAEFDAIIAAAAREAASV"
     gene            complement(15880..17334)
                     /locus_tag="VFZ86_07965"
     CDS             complement(15880..17334)
                     /locus_tag="VFZ86_07965"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HEX5642258.1"
                     /translation="MNHADRRYVGRRRPLAIIAASEVESIHAAALDVLAETGAMFHSQ
                     RALDVLEAHGATVDRETTVARIPASAVEEALATVPRSFTLGGREPEFDLPIDGEHAYL
                     CSDGCATKVREADGTVRQSVKQDVYDAARVVEGLDNLSATSALVSAQDTPEESRVLHE
                     FDACMRASRKHSVVVSIKDAAEARPLIRMAEAAAGGAEKLKARPTFSVILCTVSPLHM
                     ERFGMELALELGDAGIPMLLYPMPILGATSPVTPAGTAVVGAAEILAAITAIQLSSPG
                     ARLVHAGGPTALFMRTGSYFANVPEALLLRAVQGQMARYWGLPAGLGWGGTKAKQPEA
                     QSAYENTLGLVLEMMAGADFCFGAGLLDSVQQMSLEELVIADEVFGMVTRLLRGVTVD
                     RETLAVDLVKKLGFRGDYLFEQHTRAHVRELWQARLGETGTYESWKSAGSPRLEDKAR
                     AVADGILASPAPEFPEDLAREFDDIIAAVESGRF"
     gene            complement(17331..18434)
                     /locus_tag="VFZ86_07970"
     CDS             complement(17331..18434)
                     /locus_tag="VFZ86_07970"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEX5642259.1"
                     /translation="MTLPRLTIDLDAVADATRRLAARLRAFGLTLCGVTKCVDGEPAV
                     GQVLLDAGCAGLADSRLPSLARLAAQGLGPLTLIRAPQWDELAAAAQVADRVLLSDPG
                     AARVLGEHAPGASIQVLLTVDLGDRREGVLPDAAPAVAAELAGLPGIDLAGVAANFAC
                     LSGQLPSQALFREAEDVLADIAGLCTAEPVLSLGGTCVVQHLDGYAPRFATEVRSGGG
                     PMYGHDFVSGCGITGLGRTDPVLTAAVLECYRKPPAPQGPGGLDAFGHVPAVDLPGED
                     AWYAMLALGRRDMEPGGLRPLLPGARLAGATSDVCILITPGPLRPGDEVSFAVDYDAL
                     VRAVTSPFVRVDYAHGGESAAPTDNDVSKGDAG"
     gene            18761..20125
                     /gene="lysA"
                     /locus_tag="VFZ86_07975"
     CDS             18761..20125
                     /gene="lysA"
                     /locus_tag="VFZ86_07975"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEX5642260.1"
                     /translation="MPAADPLYTVCDGHLCFDGVDLVDLADTHETPLFVFSERRLRAN
                     VEAVIGSFRRLHPPTEVFYASKACSNLWFLRVAREAGANVEVNAGGELDKALAAGFEP
                     GQIVFNGVAKTRDEIGRAIACGVRAFMVDSVYELGRIGEVAAGMDRVAAVAPRIDVHV
                     PTLTHPGLETAHGGKAGIDRDDAAAAFRLAAEHPHLEPAGMHLHIGSQITSVEPYRQA
                     IAAALDLVAEVEASAGVRLRFLNAGGGFAVPYREAPVACHPSDYFCSLLTAADYAEVV
                     GGALAARRPDLALFLEPGRSIAATTAVLVARIENEKVKGRRDGARRRIGDERWVTVDA
                     GFNTLLEHTNYHWYYRSIVASRAGEETDTEFRLAGPLCDGGDVFAGDGDSPYRRFPAA
                     TAVGDVVAFLDTGAYTLEMMNPYNARPTAAAFAVTAAGRLVPIRRRDTAEDLVAHDLV
                     PPAG"
     gene            complement(20129..21274)
                     /locus_tag="VFZ86_07980"
     CDS             complement(20129..21274)
                     /locus_tag="VFZ86_07980"
                     /inference="COORDINATES: protein motif:HMM:NF024083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HEX5642261.1"
                     /translation="MSRRHWAHVVAAAVVAVACAAAAPAAASSPAAGAAAAEAAAGPA
                     AAATAYLEARATAVTAADPAAALAAWVVPGSALARTEAQVAAGAALRGRSAGRTVESA
                     ACAVTVLDERPAGDGSVVVVARAVTRLTWRSPNGMTDVEGSGIHHTITLDHDAGSWVV
                     TADAYSDVLKAACLEAAGAARTRVRTAARAVERTATPLALPRRSVRRPALPARRYTAI
                     IDYDRPAAQAYADKYALSYNPTFVRFSGADCANFASQCARAGDMPQSRGATSTDWWYD
                     KAYTSSPGDDTYSLSWINVTRQIASWNTRRTDWETSAGMLSRGDFIYYDWSGDGVWDH
                     VAVVAGTNSAGQKVIDAHTTDHYRVYWKLGTSSTRYRYAKVRAQWVV"
     gene            complement(21295..21801)
                     /locus_tag="VFZ86_07985"
     CDS             complement(21295..21801)
                     /locus_tag="VFZ86_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642262.1"
                     /translation="MSPASTTALALAALAALALLGPGCSGSGTEPVVVPSDVHLASGP
                     PAQASAAAAPAIVSEAVDRLRAARLVALYCHLVDRGQHARAGELCARRRLWSRRALAG
                     VTRFHFRSARVHAAPDARTLVLKARVHVHAGRGRPLPDGLAVLFFTLGRAGSAVGGWL
                     ITAISTRP"
     gene            21916..22407
                     /locus_tag="VFZ86_07990"
     CDS             21916..22407
                     /locus_tag="VFZ86_07990"
                     /inference="COORDINATES: protein motif:HMM:NF019292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HEX5642263.1"
                     /translation="MTGPDDNLPGAPLAQPPHGFWPTVRRDLRLGVIAGLQTFWDLAK
                     VMIPAYGVTLALEQLGVIGRLADVARPLMQALGLPGDAAVPLVVGYVLNIYAAVGAMQ
                     ALDLTTGQVTVIAIAILIGHNLVVEGAVLHKAGMNGFAFGALRIVAGLVAAGVANLLM
                     GLV"
     gene            22407..22877
                     /locus_tag="VFZ86_07995"
     CDS             22407..22877
                     /locus_tag="VFZ86_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HEX5642264.1"
                     /translation="MSAFLREFTTGSLDLLLRVFLIVVPIMVVLELFEGTRAFRAVVR
                     AWARLVRHVGLDERSAAPTLVGFLFGLAYGGGVIVRDVRRHDLGRRQVFIMSVFLSMV
                     HAIVEDSLIFIALGASVFWVVGYRILWAALVTLALTALAGFWLRRRRGSGSLGG"
     gene            23015..23446
                     /locus_tag="VFZ86_08000"
     CDS             23015..23446
                     /locus_tag="VFZ86_08000"
                     /inference="COORDINATES: protein motif:HMM:NF014173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX5642265.1"
                     /translation="MKSRYAVRALTELARVEQTQLGKPVRLGEIAERRAMPLQFLEQV
                     FAALRRGGIVHSRRGAAGGYALARPADDITVLDVVAALDGVPSPAECTQGRCDQVDRC
                     GASSVWIDAQHALERVLTATTIGDLLRREDALRESAPMYHI"
     gene            23463..24407
                     /gene="cysK"
                     /locus_tag="VFZ86_08005"
     CDS             23463..24407
                     /gene="cysK"
                     /locus_tag="VFZ86_08005"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015356453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEX5642266.1"
                     /translation="MKRAGNITELIGRTPLVRLNRISDETGAEVLGKLESFNPGGSVK
                     DRIGLAMIDAAERDGIIAPGRSTLVEPTSGNTGVALAMVAAARGYDLVLTMPDSMSLE
                     RRALLKAYGARLVLTPGAEGMTGAVDRAVELAGELGDAFVPQQFENPANPDVHGRTTA
                     EEIWDDTGGEVDVFVAGVGTGGTLTGVGRVLKERRPGLLVVAVEPSDSQVLSGGPAGS
                     HRIQGIGAGFVPKILDQEGYDEVLTVSGDDAFDAARRLASEEGVLVGISAGANAWAAG
                     ELARRDGMHGKVVVTILCDTGERYLSTPLFADLAEPQA"
     gene            24404..25189
                     /locus_tag="VFZ86_08010"
     CDS             24404..25189
                     /locus_tag="VFZ86_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HEX5642267.1"
                     /translation="MRISGHERRRYDCQLVLKEVGERGQQAIRDAAVLVVGAGGLGAP
                     VLYYLAAAGVGRIGIVDDDVVELSNLQRQILFTTADIGRPKAVVAAERLRALNPEVTP
                     EAHTSRLRAATAFALIDAYDVVVTAVDNFPTRYLLNDACVLRGKTLVDGAVLRMTGLA
                     MTIKGGETACYRCLFPEPPPPAGVISCSEAGVLGPIPGLIGSIQALEVLKVLTGAGRP
                     LYDRLLQYDGGAMTFTEVEVSRVPSCPVCGESPTVTELVDADA"
     gene            25182..25979
                     /gene="larE"
                     /locus_tag="VFZ86_08015"
     CDS             25182..25979
                     /gene="larE"
                     /locus_tag="VFZ86_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HEX5642268.1"
                     /translation="MPDDPVLARLLAQFAGVERACVAFSGGADSSLVLAAAARALGHG
                     NVVAVTATSPTYLDEELAAARALADGLGVEHVVVATHEFDDGRFVANSRERCYFCKAG
                     LLDELVRVAAAHGGAALVDGANADDAGDHRPGMRAAAERGVLQPLLAAGIGKAEVRRL
                     ARELGLSTWDAPQQACLASRVPYGEAITPAKLAAVADAERALRALGFRQCRVRHHGDV
                     ARLEVERDELGRALELREQIARAVHAAGFTYVALDMDGFRTGSMNEA"
     gene            26100..26951
                     /gene="cysE"
                     /locus_tag="VFZ86_08020"
     CDS             26100..26951
                     /gene="cysE"
                     /locus_tag="VFZ86_08020"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006037971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEX5642269.1"
                     /translation="MIRKLFALTGVSVAACWFLGRGTRGCRPVNEVVGALRQDVHTAI
                     EEITSDVKAVQERDPAATSKVEIITSYSGLHALWMHRLAHKLSNVGVPLVPRWISQFN
                     RFVTGVEIHPGAKIGHGLFIDHGSGVVIGETAEIGDDVTIYQGVTLGGTGKETGKRHP
                     TVGDNVVFGAGAQVLGGITVGDNAKVGAGSVVVADVPKNSTVVGNPGRPVLLQGQKVG
                     IPDIDYRHLPDPVAEAIKCLVARIVEMEKELDEIHPERSGKRQQAVRETQAVLAELLR
                     FDSGAGI"
     gene            27059..27367
                     /locus_tag="VFZ86_08025"
     CDS             27059..27367
                     /locus_tag="VFZ86_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010479642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF211 domain-containing protein"
                     /protein_id="HEX5642270.1"
                     /translation="MSEVRRLVLDVLKPHQPTMLVLADKLGDLEGIHGVDITLIEIDN
                     KVENIKITLEGDDVDFQDVAEVVQDAGGSIHSIDKVSAGKRIIDEVETPQDAGASWLR
                     "
     gene            27399..28022
                     /locus_tag="VFZ86_08030"
     CDS             27399..28022
                     /locus_tag="VFZ86_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642271.1"
                     /translation="MSLPGSPSIGVRVRRQLATLRHYHEIAEVGEIARRYFAMNAFDG
                     VLTAIGVLVGGYLGGVDSPRAIFTVVLTTAVGMGVSGFYGSYLVERAERGRAMRELEE
                     STLSSLQGSTIASASRYATIVIAFVDGASPFAAALIVMIPFAFTSVISMHTAYFLAVA
                     VGLVELFFLGVFLGSISRERLWLSGLRLVVAGAIALIISLLLGGGVG"
     gene            28019..>29874
                     /locus_tag="VFZ86_08035"
     CDS             28019..>29874
                     /locus_tag="VFZ86_08035"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX5642272.1"
                     /translation="MTADHPLLAGLNDEQRKAVTHVDGPLLILAGAGSGKTRVLTHRV
                     AWLLQVEHVRPDEVVAITFTNKAAGEMKERIEGLVGPVARTMWISTFHSMCARLLRRE
                     AQRMGYKSTFTIHDEDDRRRLIKRCMEEINVDPKRYPPESISRQISDAKNQLLGPAQF
                     REQGGRFAQIAADVYELYEKRHAEMNAMDFDDLLMKTVELLEGYPDRLQHYQQAFRFV
                     LVDEYQDTNHAQYRLANLLAGAHGNLAVVGDDDQSIYSWRGADIRNILEFERDHPDAE
                     VIRLEQNYRSTQKILDAANAVVTHNRKRKGKNLWTPRGEGALVQVVEVNDERAEAQFV
                     ASEVQKLLEGEAAGAERAYRPDEVAVLYRTNAQSRVLEEQFGRYAIAYQVIGGPKFYE
                     RAEIRDLVAYLTILVNPDDTQRLLRIVNTPKRGIGATSLQRLQAHAASVGESLWQTLR
                     GAGDVPGLSAGAVNGLLAFARTMEGLQATHAGRPVAEVVRSLLDETGYEAALAAQKTL
                     EAEGRMENIEEFVGVAAEYDKRADEPSLDLFLQEISLYSDSDALADTSSLLTLMTLHN
                     AKGLEFPVVFISGMEEGVFPHQRSLDEQNVEEERRLAYVGITRAMDRLYLVHA"
BASE COUNT         4077 a        10725 c        11079 g         3993 t
ORIGIN      
        1 aagccgccgg ccggcgagtg aaggtcggcg gtcgcgtcgc gaccgccgac ctgcgcatcc
       61 ctcgcggcga gcgctgcccc gtacggcgac gccgcggtga ggaccggagg cactgagccg
      121 gccctcaccg cggcgtcgcg gtgacccggc cggatcgcgc gtcgcggcgc gcgccggggg
      181 gtccgagacg cgcggcacgt cggcgcgcgg cccagccggg tcgctctctt gattcccccg
      241 ttgccgccgc cgcacgccgt tgacgccacc cgcgggaggc atcacaggtt cttcaaagtc
      301 cggtgctaag gtctgagcgc gtacgacgtt agagaggatg gtgtcgcggt ccatggcgtg
      361 ggaggtacaa gcggaggtcc ccggaggctt cgccggcgag gcggggctgg tcgacggcct
      421 gcgcgcgggc agtggcgagg cgttcaaggc cttgcacgag gtctacgccg ccccgatcta
      481 caacctcgcc ctgcggatcg tcgacgaccc ggaagaggct caggacgtcg cgcaggaggt
      541 gctgctcaaa gtgttccgcg ggttgcccgg caaagagacg gagctcaacc tggccgcctg
      601 gctctaccgc gtgaccgtca acgcggcgtt cgaccacgtg cgcgcgagca agcggcgtcc
      661 ggtcaccgtg atggacgagc aggcgcccga ggcgccggcg gccgttgacg agtacgagcg
      721 cgctgagctc gcgcgacggg tggagaccac cctgcgcgag ctccccaagc ggcagcagct
      781 cgcgctggtg ctgcgcgacg tgcacggcct gagcgtgggg gagacggcct ccgtgctcgg
      841 gctcacgaag gggtcggccg acgtgctgct ctcgcgagcg cgcgccggct tccggcggac
      901 cttcctcgcc ggcgcggggt cactccgcgg acgctgcgac agagccgaca aggcgctcgc
      961 cgccggcgtc gggcgcggcg agcccgtgcc ctccaagctg ctcgagcact cacggacctg
     1021 tcccgactgt cgccgcgccg tcaagctctg gggcgtggcg cccgtcggtc tcgggctgct
     1081 ccttccccaa gtggcgctgc cggccaagct gagcctcggc gcgacgctgg cggccgccga
     1141 ggcggccggg atcgccgtgc cggccgggct cgcggccgcg tccgccgccg gcgcgtcggc
     1201 cgccggctcg gcggtcgctg gatcggccgc cgccggggcc gccgccggca gcggcgccgc
     1261 cgcgacagcg ggagggaccg gcttgctggc cgcgctgggg tccgccgccg gcgtcaaggt
     1321 cgccacgctg gcggcggtgg ccaccgcagc cgtgagcatg acgggcctcg ccgcccggca
     1381 cgcggacgcg ccggccggcg tgcgcgaccc gcaggccggg ctgcggcagc aggcgggcga
     1441 gccgggacgg gacggccggg ccgggcgccg cgaggccggc ctgccggaac gcgagctgca
     1501 gcgtgcgccc cgccgcaatg cgggggacgg tccgggcggt ggcggcatcc gcgcggagga
     1561 gaccggcgcg agagggaccg ggtcgaacgt ctcgggcgtc tccaccgcgg gagcggggga
     1621 tggcgccggg tcgacaggat cgacgaggga cgcggggcgc tcgtccggcg gggacaccca
     1681 ggcgagcggc gcgggcggca atacgcagaa cacggtcgcc ggatccggcg ccggcagcgg
     1741 tcaggcccca ggcgggggcg gagacgctcc atcaggcgac gccgagaccg gcgggaccgg
     1801 aggctcgggc ggcggatccg ccggagggac cgtcagccgg gagtgacgga cgcgcgcacg
     1861 cgcagcgccg cgcgcgcgac gccggcggtg acggggacgt caccgagcgg gtcgccgtcg
     1921 acgtcgagcg ggaagtagcc ggcgcggggc cggcgtcccg ccggcggact cgccggtcgc
     1981 agttcgacct cccggcaggc gaagtggtcc acgccgggct ccaggaggtg cgtgcccgcg
     2041 tacacggtgg tgaggcgccg gacgaggcgc cagcgtgact tcgtctggac cacgatcacc
     2101 tcgagcagtc catcgtcgag cttcgccatg ggagcgatgt tcatgccgcc accgaaggta
     2161 cgcccattgc agatggcgac cgcctgcgcg tccaccagga gctcccggcg ctccccgtcc
     2221 gggctcacga agccgcagac gagcttgacc cgccggcaca tgacgatggc gcggagcgtt
     2281 gcctgcgcgt aggcgagacg gccgtcgagg aacgcgggcg cgcccgccgt gtaccggtcg
     2341 accaggccgc cgacgccggc ggagagcacg ttgatccagt gccgctccac cggcctgccc
     2401 tcgagatcgg ggaagcgcgc ccagccgatg tcgacggcgc ggtccgcgcc gtctctgagg
     2461 gcggagaggt aggcgccaag ctcgtgcggg atgccgagcg accgcccgaa gtcgccgccg
     2521 gtgcccgttc cgacgatgcc gagggcgggg aggagggcgg cgtcccgagg tccgtccgcg
     2581 agcagcccat tgacgatctc gtcgagcgtg ccgtcgccgc ccagtcccac gacgaggggc
     2641 cgccggtcgc gcgcggccgc ggccgccagt gcggtcgcgt cgccccggcg cacggtctcg
     2701 gccacgtcca ggtcgccgac ggcgccgcgg accgcctgga tgagcgacgc caggcttcgt
     2761 ccggtgcgcc cgcggccggc cgccgggttg gcgatcacga gagggcgcat ctccccgcct
     2821 gcgctgtgct ccgcggcctg catgccgcga accgtaccag accccggtcc gtcgacgcga
     2881 ggcagggcga agacatgtct attacacgga gtaactctat gattgagtta cttggcatat
     2941 gggcaaatat cgtgtgtcgc ccaggcgacc gcacccagac aaacgagtac cagggggttc
     3001 ccgagatgtc ggcttcgatc accaccagca agaggtacct cgccctcgcc gtggcgcttc
     3061 tcgcgctcgc cgtgggcttc ttcgccttca cggcgagcgc tcaggcacag acgctccagc
     3121 tcgccggggg atacacgcag ttgaccaccg atcccggcac caccaaagcc ctcgtcgggg
     3181 cgcggatcct gccgcttccg atcctgccgt cgtgggtgat cccgaccacc tcgaacggtc
     3241 agctcgcgct gcgctaccgc ttctacatca ccggcggcca gatcgacggc cagacgctgg
     3301 gcggcgagat cctgcactcc ggcgggctca agttcgccaa cctgaagaac ggcaagtcgc
     3361 tcgcggtcaa gcgcttcacc atcgacacga acgccaagca gctcacggcc tacatcccgg
     3421 cgctcggcgc ccgcgcggcc atcctcgacc tcgatctgtc cggcgtgcag gtcagcgccg
     3481 gcaaggtcta caccaaggtg ggcccggtgc cggccaagct gacccccgcc gccgccggcg
     3541 cgctcaacgc cagcctcgga accaacctct tcaccccggg tctcgccctc ggcacggcct
     3601 acgtgtacgc gcgcttcgcc aactgaccca ccgcactccc gccggacaca gagccggtgc
     3661 gaacggtacc ccaggagcgg cggcttcgcg cgaagccgcc gctcctggcc gcgtagaccg
     3721 ccgcgagatt cgtccgattc cgggcgaaac cccctcaagg atcgcctctc tggcgatgcg
     3781 ggccgcgcgt ctggtatgaa tggcgacagc gacgggggag gcagcgcatg acgtccatga
     3841 ggcgaccgcc gatcgagccc atcgagcggg cgttcgcatt cacctatcac tcggtggagc
     3901 agctcgaccg gctcaagcag gcgacgctgg acatcctcga gaccgtcggc gtgcggttcg
     3961 agagcgccag ggcgctcgac ctcctcgccg aggcgggggc tcgcgtggac cgggtgcgtc
     4021 aggtcgccaa gctgccgccc gccctggtgc tcgaggcgat ggcgacggcg ccgcgccgct
     4081 tcgtcctcgg cgcccgtgac gcggagtgcg acctctccgt cggcgacggc cgcacctact
     4141 gcaccacgga cgggtgcggc gtcgagacca tcgacttcca cacgcgggag cggcgccggt
     4201 ccaccaaggc cgacctggca gatgtctccc gcctgcagga cgcgctctcg agcatcgcct
     4261 tctggtggcc gacggtgagc gccggcgacc acggggagac ggcgcagctg cacgagctcg
     4321 acgccggctg gaacaacacc gtcaagcacc tgcaggggat ggtcaacggc gagcgggagg
     4381 cccgctacgc cgtggagatg gccaccgtga tcgccggcag cgccgaggag ctgcgccgcc
     4441 ggccggtgat gagcgacctc atcggcacga tctcgccgct cgtcgtggac aaggacgcga
     4501 tcgaggcggc gctcgtgttc gccgccgccg gcgtgccggt cacgtgggtg accatgccga
     4561 cgctcgggac caccgcgccg gcgaccaagg ccggcgccta cgcgctcggc tcggcggagc
     4621 tggtcgccgc gaccgtcgtg ctgcagctgg ccagccccgg cgcgccggtg ctgcactcga
     4681 ccatgcagag ctgggccgac ccgcgctccg gagacttcgt gagcttcccg ctcgacggcc
     4741 ggacgcgagc cctgggcacg gagctcgcgc accactgggg cgtgccggcc gagtcggccg
     4801 cctgcggcac cgactcaccc gtccccggca cctggcaggc cggcgtggag gaggctctgg
     4861 acctggtcca ggcggcccag gagggctccg acctcctgcc gagcatcggt ctcgtcaacg
     4921 tctacacgct gttcttcccg gagcacctcg tcctcgggga cgacgtctac cgccgggctc
     4981 ggtacgcggt catggacatc gacatggacg acgagtcggt ggcgctggac gcgatcgccg
     5041 aggtcgggcc cgggggccac ttcctcggca accggcacac gcgccggcac atgcgcgacg
     5101 cggtggtccc ggcgctcacg caccggctgg ggccggacgg ggctccccgc gaccccgtgg
     5161 cggtggcgcg cgagcgagcc gaggagctct ggcgcgagta ccgcccggag cctctggagg
     5221 aggacaaggc ggccgagctg gcgcggatcc tcgcggcggc cgacgccgag ttgcgcggct
     5281 agcgtgcgcc ggccgcgccg cgaccgtacg acctgatgcg tggtcccggc tcgcccgtgg
     5341 aaagctcctc tcgtggatcc gaggagagga gcgtcatgga cgagaacgtc aacgccacac
     5401 agggcgaccc ggcgggtggc gaggtacgcc acggtctgat cgggatggtc cgcgcggacg
     5461 aggtggacct gcggcagagc gcgtcctgga tcgcgaccgc cagaggcggc gcggacctgc
     5521 accagagcgc gtccatcgcg ctcgtctcgg gcggcgacac gaccatgaag atggccgcgg
     5581 ccgtcatggt gcccacgctg ggcgacgcgc acctggagaa gtccggggcg cagtgggtcg
     5641 tcgccgccgg cgacgtcagt atcgagaagg gcgggtgcgc cgccgccgtg gcgcccaccg
     5701 tcaaggtcga ccacggtgcg gtcggcgtgg ccctcggctg gcacgtggac gtcggtgagg
     5761 gcggccgcgt catgttcgga ccgctggccg gcgccgcgtt cggcatcgcg ttcggcatcg
     5821 gagccggcct cgtgatggcg ctcggcgccg gctacgccgc gaagaaggcg ctggggcgcg
     5881 tgccgcagct tccctggcgg gcgtgaccgc ggcggccgcg gacggccgcg acgcggccgc
     5941 ccgggcggag gcggtcctcg ccgagctcgc gtcgctcggc agcgaggagg cgcgggcggg
     6001 catggcgcgc tacggcatcg agaccggcga cgccttcggt gtctccatct ctcagctgcg
     6061 tcccctggcc aaggggctcg gtcgcgacca cgacctcgca ctcgccctgt gggccacggg
     6121 caaccacgag gctcgcctcc tcgcgagcat ggtcgacgac ccggcctcgg tctccgcgga
     6181 gcagatggat gtgtgggcgg ccgcgttcga ctcctgggac gtgtgcgacc aggtcacctc
     6241 gaacctcttc gacaagacgc cgcacgcctg ggacaaagtg caggagtgga gcgccgcccg
     6301 ggaccagtgg gtgaagcggg cggcgttcgc catggcggcc gccctcgccg tgcaggacaa
     6361 gcaggcggcc gacgggccct ttctcgagat cctggagttg tgtcgccgcg aggccggaga
     6421 cgaccgcaac ttcgtgaaga aggccgtcag ctgggcgctg cgcaacatcg gcaagcggaa
     6481 cccgcggctg cacgcggcgg cgatcgccac ggccgaagcc atcctggccg aggccgagga
     6541 gcgcgccgcc gcctcccgca aggacccggc cgcgcgcagc ggccgctggg tcgccaggga
     6601 cgcgctgcgc gagctgcgca gcgagaagac gctggagcgg gtgcggcggc ggggatgagc
     6661 cgccgctgcg cggcggcccc cttcgcggcg cccctccgcg ccgtgggcgc ccgtggcggc
     6721 gtcgagcgtg acgtagtacg gtacaggtct gcgtgagcct gtcccgtcac gaggccctgg
     6781 ggggtcagat gccggtcggc gcgaccagca cctcgttcga tgccatccgg cgccgtcatc
     6841 cccgggtgct gcgcaccgtc acctggctgc ggtcacatcc ggtcctctgc ggcatcgccg
     6901 gcaccatggg gatcgccgcc gtgttcttca tcgacatgtc ctatcccggg gtcctcgtct
     6961 acgggctcta ctacgtcccg ctgttcttcc tcgcgctggc ggtcaactgg cgcgcggtgc
     7021 tgttcgccgt cgccctctgc atcgcgctct cggcgttcgt ctacttctgg gaggaatcgc
     7081 tcgacacgga ccgggggttc atcctcctgt acatggcgat gatcggcgcc gcgctggtca
     7141 tgctgtccta tctgatcaag cggctgtcca cgaccaccga gtacgccgag ctccgcgccc
     7201 agctgtcgga ggcgggggcg gacatcctcg gcggcggccg ctcgcgcgac gacctcgacg
     7261 agctgctcga gtacgcgctg gagcgcctcg gcgagcagct cgacgccacc aacggcgtcc
     7321 tcctcctgct cgacggcggc gagtggaggg gatgggccgg cttcggtctg ggcgtggacg
     7381 cgcgccaggt ggccgcccgc cacgccgacg tcccgctggc ggcccaggcc ctgcgcgcgg
     7441 acgccgccct gtcgcgcgac ttcacgaacg gcgacccgtc gccgctggcg ccgctcgccg
     7501 cgcacgtgcg gctcgagcgc gtgctcgtca tccccatgcg ctcgctcgaa cgcgaggtcg
     7561 gcgtcctggt ctacaaccgg ccgcaggagg cgggcgagtt cagcgacgag caggtgggcc
     7621 tcgccgaggg cctcgcccgc tacgtcggcg tcaccgtgga caacgtgcgc ctcatggtcg
     7681 agctcaactc gcggcggcgt gacctcgagc tggtgcgcga ctcgagcctc gacttcgcgc
     7741 agagcatcga catggacgag gtcctcgagg ccgtcgtgag gcggctcgtc ggcgtgctcg
     7801 acatgcgcgc ctgcgacatc ttcgaggtgg acatggacgg cggggtgatg cgcaacctcg
     7861 tgacctacga ggacggcgcg ttcgacaccg gcgagcgggt gggccgggaa tatcccatcg
     7921 actacttcgc gacgagcgcc atggcgatcc gcagccgccg tcccgtcatc gtgacgtcgg
     7981 tcgacgatcc gcggctcaac tacgcggagc gcgactacct gagacacctg gggcgcaaca
     8041 cagagctggt catcccgctg cgcatccgcg agaacgtcct cgccctcgtc aagctgctgg
     8101 acgacacgga gggtcgcgac ttctcgaacg aggaggtcga gctggcgcgc agcatctgcc
     8161 ggttcgccgc gctggcgatc gacaaggcgc gcctctacga ccagcagcgc gccacggcgg
     8221 agcggcgcga caggctggcg cgccgcctgc agcgcctgca gtcgttcgcg gtcgacctca
     8281 accagcggct cgaccgcgcc gacctgcagg aggtgctcga cgaggtcacc cgcgccgccg
     8341 tcgacctcct gcgcgcgcgc tcggcggcgg tcgtcagcgg gagcggggac ttcgtcgccg
     8401 tgcggtcgct ggcggtggcc ggcagcgcca ccccgggcgg cctcgcggcg gccgaggccg
     8461 acctgatcga gcgctgcacg ccggggatgg cgccgggggg cgaggacttc gccggcgccg
     8521 gcggcgtcgc cggcgagggc gggcacgacg ggcgcctgct cctggccccc ctggcgggcg
     8581 aggcggcgcc gcagccgtcg gccctcgtgg tcgccgacaa ggaacagggg gagttcgacg
     8641 gcgaggaccg cctgctgctc gccacgctgg cggcgcagct caacgcgagc atgcacaacg
     8701 ccatcgccta tcagcgcgag cacgtgatcg ccgagacgtt ccagcaggcg ctgctcatgg
     8761 agccgccggc catccccggc atcgaggtgg gcgtccacta ccgcgcggcc accgactccg
     8821 cgcgcatcgg cggcgacttc tacgacctcg tgtcactcgg tccgggcaag ctgatggtga
     8881 tcgtcggcga cgtgtgcggc aagagcctgg cggctgccgc ccagtcggcc gtggtccgct
     8941 acatgctgcg cgcctacgcc gccgagggct ccccgggaga ggctctctcc cggctcaact
     9001 cggcggtcat cacgcagacc cccaaccagc cgttcgtcac gctggtggtc gcgtacgtgg
     9061 acgtggcgcg gcacatgttc gagtacgccg tggccggcca cccgcggccg atcgtgctcg
     9121 ccggtctcga ggagttcccc atgcccggcg agggcaacgt gcccgtgggc atcttccgcg
     9181 gcgtcacgta ccccaccaac cgcgccgtgc tgcccgacga ctcgtgcgtc gtcctctaca
     9241 cggacggcat caccgaggcg cggcgcgacc gcgtcctctt cggcgaggag cggctgcacg
     9301 aggcggtgcg cgccaaccgt ccgctggacg cgcaggcgct cgcggacgcc ctcctcgaga
     9361 cggtcaagga gtactccggc ggggtgctcg cggacgactg cgccgtcgtc gccgtcaagc
     9421 tgccgtaggc ggcgtggccg gcggcgggac gccgcgtcgc cgcggccggg cgacgctcag
     9481 gaacctggtg aaggcgtcca cgtcgatgaa gcccagcagg cgcccgccgg gatcgaccac
     9541 cacggccctg tcggactcgc gctcgcgcag caggcggagc acctcgaccg cgccggtgtc
     9601 cggcgccacc gccggcattt cgggcgcggg cgtcatcacg tcgcgcacgc ggtcccggtc
     9661 gtagcgctcg cgcgccgcgc gccggagatc gcgcgagcac atgaggccgc cgtactccgc
     9721 gtcggggtcc tcggcggcga ccggcaggca gcgcgtgtcc cagtccccga gccggtccct
     9781 ggcgacccag tcgagggtca tgtacgggga gacccagctc ccgggcgtgg tcatcaggcg
     9841 agcggccggc acgccggcga gccgctcctc gatcagcgtc tgccgggccg tcgccctcgc
     9901 cgcctggatg agcatccacc cgacgaggat gagccagatg cctcccaggc cggagaacag
     9961 gatctgggcg acgcccaggg cgatgaagcc ccaggcgaag acctgtccgg ctgctgccgc
    10021 ccacgtggtc gcgcgcaccc ggtcgcgcgt gatccgccag aggagggcgc gcagcacccg
    10081 gccgccgtcg agcgggaagc ccgggaggag gttgaaggcg accagggcga ggttggtgat
    10141 gcccaggtac gtgagcaggg cgaccagctg ttcgggaccg ggcagcgcga aggccgcgcc
    10201 gagcgtcgcg acgccgatgg ccacgctcac gagggggccg gcgatcgcca tccaggcctc
    10261 gcggccgggg gagtcgggct cgcgggtcag ctgggcgacc cccccgagga tgaacagcgt
    10321 gatgttgcgc accgggatgc cctgcgagcg ggccacgagg gagtgcccga actcgtgcgc
    10381 gagcacggag gcgaagagca gcagagtggc gagcacggcg atcgcccagg tggtctccgg
    10441 gctccatccc gggtaggcgc gcgggaagaa ccccgtcgcc agtgagaacg cgaagaacgc
    10501 gagcacgatg aaccaactgg ggtggatggc cacctcgatg ccgaagatgc ggcctactct
    10561 gatgccgttc acggtctcct cctcacacgt gttgcatagg agttgttcca cgctccaaac
    10621 gcggaggaac cacgggcggc ggcggggcgc cgggtacgag gtcgcgggga ggagtcagcc
    10681 ggccgtcacg agcgtcgtca tggcgtcggc cacgacgagg accgaggcgc ggcgggcgag
    10741 ccgctcgtcc tcggcggcga gggccgcgtc gacggaggcg aacgcggcca ccccgaggtg
    10801 ggcgagcggc tcgaggaagc gtgcgtcgtc cgctcccacg accgcgaggc ggtggcgttc
    10861 gagcacgcgc gcgacgacgt acgcccgctg gccgccgggg ccgagcggct cgcggagccc
    10921 gcgctcgagc agttcggccg gcgacgcggc ggcggccagc acctcgagga agttgcgctc
    10981 gccgggtccg tcgccggcgc cgagcggcag gtcggcgcag agcacgagga ggccgccgtc
    11041 cgcgagcgcg gggcgggcgg cgaggccgag gtaggtcgcc gccctgctcg cctggtacag
    11101 gccgtcgctc ttgggcgcgt gcacgccggc gacgaccacg tcgtattgct cggcggtggt
    11161 gcgcagccac cccggcgccg cgtcccgcgc gagcgcctcc tgcacggatg ccgggtcgcc
    11221 ggcgcggacc gccacggcgt cgccctcctc catgacgagg ttgacgcccc agccgagcgg
    11281 cgtgcgcgcg gcgacctcgc ggagcgtcgc ctggaagggg ttctcgcgca ggcgggcgac
    11341 ccggacgccc ggccgcgaga tgaacgccgg gcggtgcgtc caggcgatcg tctcacgccc
    11401 ggcgcagccg atcgccacac ccttgacgcc gccggagaag cccgcgtaga ggtggggctc
    11461 gaccacgccg gccgtcacca cgaggtccgc ctccgccacc cggcgcgcga tcctggccgg
    11521 cgcgcccagc gaggtctcgc cgaggtccgc cgtctccgtc tcgatgcctt gcgcgtcgaa
    11581 cacctcgacg cggcggacga cctgcgggcc ggccagccgg gcgcgctcgg cctcccccgt
    11641 ggtggcgtgc aggccgcagc cgatcgcgac ggcgacggcg tcgtcgcgca cgcccgccgc
    11701 cgccagctcg tcgagcagcg ccagcaggac gggctcgctc gggcagggcc gcgaggcgtc
    11761 cgggatggtg acgacgaccg agcgggcgcc gcgcgccagc tcacgcaggc gcgccgcccc
    11821 caccggcgcc gccagcgccg cgcgtacggc gaggtcgagg tctgcaaggg gcgccgcagc
    11881 cgccggcggt tcgaagcggt ccgcgcgggc gatgggcagc cgccgcgcgc cgcaccacgc
    11941 ccaggccggc gcgttcaagc ggcgggcgcc gcgccggcgg cctcgcggga ggcgcggcgc
    12001 cacgtccacc acatgaccac gacgccgacg aacgggagca gcgaccagag aaagccgcgg
    12061 acgagccagt cgagcaggtc gccgcgggtg cgcctgcagg ccgaccaggc cagccacatg
    12121 cagatgacca gctgcggccc gtaggcgaag aacgcccacg cggcgaggta ggcgccgtag
    12181 gcgacggggt tctcgctcac cggtaccggt ccagcagacg gcggacggcg gcgcgcaggc
    12241 cttccaccgc ctccggcggc tccagcgggc gcgcctggtc ggagaaacgc aggaggtgag
    12301 ccgcgagcca gcggtcgttg acgtagggct ggctcgccac gcaggcgcct cccggcagtt
    12361 cttccaccgg ctgacgctcg gcgatccagc gcgcgacggc ggggctgtac cagaccgccg
    12421 cctccttggg cgcgtagctg ccggacgggg ggatgccctc gcgcaggtag aggccgagct
    12481 ccacgtcggc gcgcggcgcg aaggtctcgt cctcgagctt gacgctcttg gtggtggcga
    12541 cgcggaacac gcgcgtcccg ccggcggtgc ggcaccagca gacgtagtac cactcgccgc
    12601 ggctgtgcac gagaaggtac ggctccacca cccggtcggt gaccctgtcc gtgccctccg
    12661 tccagtactg gatggagagg aggcggcgct cgacgatcgc ccggttgacc gcggtcagca
    12721 cgtcgtcggg ggccagcagc aggtcgccgg cgccgagcgc gggggtgccg ccgcgcgccg
    12781 cgcgcagctt cacggccgcg ctgcggaggg cggcgccgga ggacgtcggc aggtgccggc
    12841 cgaccagctc gatcgccagg agcagggtgt ccgcctggag aggggagagg cgggcggggc
    12901 gcgccagcgc cggacccgcc gcctcgcagg agacgtacag ccgcttcgtc cccttgaact
    12961 ccgcccagag gagacacccg tcgcctccga agttgaccac gttgagcagc ttcacgtctg
    13021 cgcgcagctc cttgtcggtc acgccgagcg ccgcgcgcac ctcggcgacc tccagcgtcg
    13081 cctcgtcctc gtcgcagcgg cgcaggaggt aggcggcgag cgccgtgagg cgggtgaagc
    13141 ggtcgacctc gaccttcatc tcgtcgtcgc ccgtccggcg ccgcttgtcc ggcttggggc
    13201 ccggcgcggt gcgcttcccg ggcgccgggc gctcgggggg tggggcgtcg aggagcgtcg
    13261 cgaggctctc gagctggccg cgcaaccggc cgcgcaggtc gtccggctcc aggagctcgg
    13321 cgtcgtccgc cagcccgagg atccagccca gcagcgggcg ggcgtcggcg tacggggtct
    13381 cgtacacgat gccgccgtcg tccaggcggg tcaccgtgcc gcagtgcgcc cagtgcgcct
    13441 cgacccacca cgccatggcc gcggagacgc ggatgcgcgc cgtcccgctc gccttcgcca
    13501 gctgccacgg cggccggtcg cggtactgcg cgagatcgaa gtccgccggc ggggagaagt
    13561 cgtgcggacg gcgcgtgtgg tacttgacct tcgactcgat gcgcgagacg cggaacgtgc
    13621 gcacggcctc gcgcaggtgg cagtgcccga tgaggtacca ctcgccggag accagctgga
    13681 ggccgtacgg gtccacgacg cgctccgtct tctggtcgcg ggtgatggcg tagtagctga
    13741 aggtcaccgt cttgcgctcg acgaccgccg cctgcagccg cggcagggcg gcggccgcgg
    13801 cgtgcgcctc cggctccacc cgcagcggcg gcgtctccgc ctcggtgagg agctcggggc
    13861 gcccctgggc gaggctgagc agggcgagcc tgagcggctg cgagtaggcg aagcggtgct
    13921 cgaggaccgc gagacaggcg gcgagggcgg cgaactcgtc gccgtccagc tgcaccggct
    13981 ccaggtagta ggcgtccgcg ggcaggctgt agacctccgt ggaggcgtcc gccagtgggt
    14041 cgtcctcgga gcggatgccg atgccgagct cgccgagctc gaagcggtcc tcgtagaagc
    14101 gccgcttgaa ggcctcgtcc gtcatgcccc agtagccctc gacccgctcg cggatctccc
    14161 cggcgctcac cggctggccg cggcgggaca gcaggagggc gacgagggag aggcggcgca
    14221 cgagcttgtc ggtgtccttg ctctgaccct cttctttgac tctcgtgggg ggcacgttct
    14281 gcgctccgga gacggacgac gagacgccgg cgccacgggc gggcgccggc gtctcatggt
    14341 accgcaggtg cagctggcgg gggaggggcg gggccccgcc gcgtcacgtg accgcagcta
    14401 gacggacgcc gcctccctgg ccgcggcggc gatgatcgcg tcgaactcgg cgccgaggtc
    14461 ggcggggaac tccggggccg gcgccgcgag gatctcggcc accttctcct gcgccttctc
    14521 ggtggtgctc ttcgcgccgg cgttcttcca gccgtcgtag ttgcccgtct cgccgagcct
    14581 ggcctgccag agctcgcgca cgtgggcgcg cgtgtggttg gcgaacaggt agttgccgtt
    14641 gaagcccatc tccttgatga gctccacggc aagggtctcc ccggtcacct cgatgccggt
    14701 gaggatgcgc ttgaccatgc cgatgatctc gtcgtccacg accatgttct cgaggcagag
    14761 gatgcgcgag ccgtcgagga ggccggtgcc gaaggtgacg tcggcgccgt tggcgtaagc
    14821 catgaacatc gccagcgcgt tctcgtaggc gctctggatg ccgggttcct tggcggtggt
    14881 ggcgccggcg ccgtaccagg ccggcacgcc gtagaagtcg gccatgtggc cctggcaggc
    14941 gcgcaggagc agggcttccg gcgagttgct ggcgtaggcg cccgagttca tgtacatcgc
    15001 cgtcgggccg ccgccgtgca tcaccggggc gccggggtgg gcgagctgta cgagcacggc
    15061 gccgctcagg aactcggcgt tgttcacgac ggccgagccg gcgatggtga tgggacccgt
    15121 cgcgccgagg atcggcatgg ggtagaagct cacggggatg ccggcctcgg ccagcgtgag
    15181 cgcgaggtcc atgccgtagc gctcctggtg cagcgggctc accgtgcaga tgatggtcgt
    15241 gaacatgggc cggcgcttga gctcctcctt gccgccggcc agcgcctcgg ccatcttgag
    15301 caggctgcgg gcctcccagt cctccttgat cgagacgacg atgctgtgct tctccgagtt
    15361 gcgcacgcag gcgtcgaact cgtgcagcac ccgcgtctcg ggcgggcagt cctgggcgct
    15421 caccacggcg ctcgtggcgc tgacgttgtc gagcgcctgc accaccttcg cgcagtcggc
    15481 gaggtcctgc ttgcgcgagg accggacctc gcccgtctcc gggtcgcggg cgaagacgcc
    15541 gcagccgtcg gagctgatgt agacgtgctc gccgtcgagc ggcaggtcgt actcggggtt
    15601 gcgcgcgccg agcacgtact cgtgcggcac ggtgctcatc gcccgctcga cgacggcggc
    15661 cgggatcttc gccaccgtcg tctcgcggtc caccgccgcg ccgtgttcct cgaggatgtc
    15721 gagcgcccgc tgcgagtgga acttgacgcc cacgctctcg atcacgtcga gcgtggcttc
    15781 gtggatctgc cggatctcag cggcgtcgag caggtcgagc ttcacgcgct tgccgacgta
    15841 gcacttgtct gcgtatgcca tcctgggctc cttgccttct cagaagcggc cggactcgac
    15901 cgcggcgatg atgtcgtcga actcgcgcgc gaggtcctcg gggaactcag gcgcggggga
    15961 ggcgaggatg ccgtcggcga cggcccgcgc cttgtcttcc agacgcgggc tgcccgcgct
    16021 cttccagctc tcgtaggtgc cggtctcgcc gaggcgcgcc tgccagagct cgcgcacgtg
    16081 ggcgcgcgtg tgctgctcga acaggtagtc gccgcggaag ccgagcttct tgacgaggtc
    16141 gaccgccagg gtctcgcggt cgacggtgac gccgcgcagc aggcgggtga ccatgccgaa
    16201 cacctcgtcg gcgatcacga gctcctcgag gctcatctgc tgcacgctgt cgaggaggcc
    16261 ggcgccgaag cagaagtccg cgccggccat catctccagc accaggccga gcgtgttctc
    16321 gtaggcgctc tgggcttcgg gctgcttggc cttggtgccg ccccagccga gccctgcggg
    16381 caggccccag tagcgggcca tctggccctg tacggcgcgc agcagcaggg cctccggcac
    16441 gttggcgaag taggaccccg tgcgcatgaa gagggcggtg ggccctccgg cgtgcacgag
    16501 cctggcgccg gggctggaga gctggatcgc cgtgatcgcg gcgaggatct ccgcggctcc
    16561 gaccacggcc gtgccggccg gcgtgaccgg gctggtcgcg ccgaggatcg gcatggggta
    16621 gagcagcatc gggatgccgg cgtctccgag ctccagggcg agctccatgc cgaagcgctc
    16681 catatggagc gggctgacgg tgcagaggat gaccgagaag gtcgggcggg ccttgagctt
    16741 ctcggccccg ccggccgcgg cctcggccat gcggatcagc ggccgcgcct cggccgcgtc
    16801 cttgatgctc acgacgacgc tgtgcttgcg cgaggcgcgc atgcaggcgt cgaactcgtg
    16861 cagcacccgg ctctcctcgg gcgtgtcctg cgcggacacg agggccgagg tggcggagag
    16921 gttgtcgagg ccctcgacca cgcgcgcggc gtcgtagacg tcctgcttga cgctctgccg
    16981 cacggtgccg tcggcctcgc ggaccttcgt ggcgcagccg tcggagcaca gataggcgtg
    17041 ctcgccgtcg atcggcaggt cgaactcggg ctcgcggccg ccgagcgtga acgagcgagg
    17101 cacggtcgcg agcgcctcct cgacggcgct cgcggggatg cgcgcgaccg tggtctcgcg
    17161 gtccacggtc gcgccgtggg cctcgagcac gtcgagggcg cgctgcgagt ggaacatggc
    17221 gccggtctcg gcgagcacgt cgagcgcggc ggcgtggatg ctctccacct cggaggcggc
    17281 gatgatcgcc agtggtcgcc gccgcccgac gtaccgtctg tcggcgtgat tcatcctgcg
    17341 tctcccttcg agacgtcgtt gtccgtcggc gccgccgact cgccgccgtg cgcgtagtcc
    17401 acgcgcacga acggcgaggt cacggcgcgc acgagcgcgt cgtagtcgac ggcgaagctg
    17461 acctcgtcgc ccgggcgcag cggcccgggg gtgatgagga tgcagacgtc gctggtcgcc
    17521 ccggcgaggc gggcgccggg gagcaggggc cgcagcccgc cgggctccat gtcccgccgc
    17581 ccgagcgcca gcatcgcgta ccaggcgtcc tcgcccggaa ggtcgaccgc gggcacgtgg
    17641 ccgaaggcgt cgaggccgcc gggcccctgc ggcgccggcg gcttgcggta gcactcgagc
    17701 acggccgcgg tgagcaccgg gtcggtgcgc ccgagcccgg tgatcccgca gccgctgacg
    17761 aagtcgtggc cgtacatcgg cccgccgccg gagcggacct cggtcgcgaa ccgcggcgcg
    17821 tagccgtcga ggtgctggac gacgcaggtg ccgccgaggc tgagcacggg ctcggcggtg
    17881 cagaggccgg cgatgtccgc caggacgtcc tcggcctccc ggaacagcgc ctgcgagggc
    17941 agctgcccgg agaggcaggc gaagttcgcg gccacgccgg ccaggtcgat gccgggcagg
    18001 ccggccaact cggcggcgac cgccggggcg gcgtccggca ggacgccctc acggcggtcg
    18061 cccaggtcca cggtgagcag gacctggatg gaggcgccgg gggcgtgctc tccgagcacg
    18121 cgggcggcgc ccggatcgct gagcagcacg cggtccgcga cctgcgccgc ggccgcgagc
    18181 tcgtcccact ggggcgcgcg gatgagcgtg agcggcccca ggccctgcgc cgcgagccgg
    18241 gccagcgagg gcaggcgact gtcggcgagt ccggcgcagc cggcatcgag gagcacctgc
    18301 ccgaccgccg gctcgccgtc cacgcacttg gtgacgccgc agagcgtcag cccgaaggcg
    18361 cgcaggcgcg ccgcgagccg tcgcgtcgcg tcggcgacgg cgtccaggtc gatcgtgagc
    18421 cgggggaggg tcactgcttc tccacttcgt ctgccgggaa cgggaccgcg gagcccgtcg
    18481 gcgtgcggcc gcgggcgtgg agagatcggg gttccagcgc ggggccggcg ccgtggcgcc
    18541 tgcgggaagg ggacgggacg ccgccggatg tcgcccgcgg gtgcgggtgg cggtgcgcgt
    18601 caggcgggac gggtattaga agtcgtggac cttaccgccg gagaaggcgg tgcgacgacg
    18661 atcgtgaggg cttgcgtctg ccacttggtc tcccgtggcg ggtggggcga agcgggcgca
    18721 gtatacttcg ctgaccgtcc atccgacaag cagggagcgt atgcccgcgg ccgacccgct
    18781 gtataccgtc tgtgacggcc atctgtgctt cgacggggtg gacctcgtcg accttgcgga
    18841 cacgcacgag acgccgctgt tcgtcttcag cgagcggcgc ctgcgcgcca acgtcgaggc
    18901 ggtcatcggg tccttccggc ggctccaccc gcccacggag gtgttctacg ccagcaaagc
    18961 ctgctccaac ctctggttcc tgcgcgtggc acgggaggcc ggcgcgaacg tggaggtcaa
    19021 cgccggcggc gagctggaca aggcgctggc ggcggggttc gagcccgggc agatcgtctt
    19081 caacggcgtc gccaagacga gggatgagat cgggcgcgcc atcgcctgcg gcgtgcgcgc
    19141 cttcatggtc gactcggtgt acgagctggg acggatcggc gaggtggcgg ccggcatgga
    19201 cagggtcgcc gccgtcgcgc cgcgcatcga cgtgcacgtg ccgacgctca cgcacccggg
    19261 gctcgagacg gcgcacggcg gcaaagcggg catcgaccgt gacgacgcgg cggccgcgtt
    19321 ccgcctcgcc gcggagcacc cgcatctgga gcccgccggc atgcacctcc acatcggctc
    19381 gcagatcacc agcgtggagc cgtaccggca ggcgatcgcc gccgctctcg acctcgtggc
    19441 cgaggtggag gcctccgccg gcgtgcgcct gcggttcctc aacgccgggg gcggcttcgc
    19501 cgtgccgtat cgcgaggctc ccgtcgcctg ccacccgagc gactacttct gctcgctgct
    19561 gaccgccgcc gattacgcgg aggtggtcgg cggcgcgctc gcggcgcggc ggccggacct
    19621 ggcgctgttc ctcgagcccg gccgctccat cgccgccacg acggcggtgc tggtggcgcg
    19681 catcgagaac gagaaggtca aggggcggcg cgacggcgcc cggcgccgca tcggcgacga
    19741 gcgctgggtc acggtggacg ccggcttcaa caccttgctg gagcacacca actaccactg
    19801 gtactaccgc agcatcgtgg cttcgcgcgc cggcgaggag acggacacgg agttccgtct
    19861 cgccggtccg ctgtgcgacg gcggggacgt cttcgccggc gacggcgatt cgccgtaccg
    19921 gcggttcccg gcggcgaccg cggtcggcga cgtggtcgcc tttctcgaca ccggcgccta
    19981 cacgctcgag atgatgaacc cctacaacgc ccggccgacg gccgccgcct tcgcggtgac
    20041 ggccgccggc cggctcgtgc cgatcaggcg cagggacacc gccgaggacc tcgtggctca
    20101 cgacctcgtc ccgcccgcgg gctgagagtc acacgaccca ctgcgcccgc accttcgcgt
    20161 agcggtatct ggtgctgctg gtcccgagct tccagtacac gcggtagtgg tcggtggtgt
    20221 gggcgtcgat gaccttctgg ccggcgctgt tggtgccggc cacgacggcc acgtggtccc
    20281 acacgccgtc gccgctccag tcgtagtaga tgaagtcgcc gcgactgagc atgcccgcgg
    20341 acgtctccca gtcggtgcgg cgcgtgttcc aggacgcgat ctgccgcgtg acgttgatcc
    20401 agctcagcga gtaggtgtcg tcccccgggg acgacgtgta ggccttgtcg taccaccagt
    20461 cggtgctcgt ggcgccgcgg gactgcggca tgtcgccggc gcgtgcgcac tgcgacgcga
    20521 agttggcgca gtcggctccg gagaagcgca cgaaggtggg gttgtacgac aacgcgtact
    20581 tgtccgcgta ggcctgagcc gccggccggt cgtagtcgat gatggctgtg tatctccgcg
    20641 cggggagcgc cggcctgcgc accgaccgcc tgggcagggc cagcggcgtg gcggtgcgct
    20701 ccaccgctcg cgccgccgtg cgcacccggg tgcgtgcggc gcccgccgcc tcgaggcagg
    20761 cggccttgag gacgtcggag taggcgtcgg cggtcacgac ccagctcccg gcgtcgtggt
    20821 cgagcgtgat ggtgtggtgg atgccgctgc cctcgacgtc cgtcatgccg ttcggcgagc
    20881 gccacgtgag cctggtgacc gcccgcgcca cgacgacgac ggatccatcg ccggccggcc
    20941 gttcatcgag cacggtgacg gcgcaggccg ccgattccac cgtgcggccc gcgctccggc
    21001 cgcggagcgc ggcgcccgcc gcgacctgcg cctcggtgcg cgccagggcg ctgccgggga
    21061 ccacccaggc ggccagcgcc gctgcggggt cggcggcggt gacggccgtg gcgcgcgcct
    21121 cgagataggc ggtggcggcg gcggccgggc cggcggccgc ctccgctgcg gcggccccgg
    21181 ccgccggcga acttgccgcc gccggcgccg cggccgcgca ggcgaccgcc accacggcgg
    21241 ccgccacgac gtgcgcccaa tgccgtctgc tcatactgac ccctttctgg agggttacgg
    21301 gcgggtggag atggctgtga tcagccagcc cccgacagcg ctgccggccc tccccagggt
    21361 gaagaacaga acggcgaggc cgtccggcag cggacggcct cgcccggcgt gcacgtgcac
    21421 ccgcgccttg agcaccagcg tgcgcgcgtc gggcgccgca tggacgcggg cggagcggaa
    21481 gtggaaccgg gtgacgcctg ccagagcgcg ccgcgaccag agccggcggc gcgcgcacag
    21541 ctccccggcc cgggcgtgct gaccacggtc gacgaggtgg cagtacaggg cgacgaggcg
    21601 cgcggcccgg agcctgtcca ccgcctcgga cacgatcgcc ggcgctgccg cggccgacgc
    21661 ctgcgcgggc gggccggacg ccaggtgcac gtccgacggc acgaccaccg gctccgtccc
    21721 gctgccgctg caccccggcc cgaggagcgc cagggcggcg agggcggcga gggcgagtgc
    21781 ggttgtgctg gcgggcgaca tgccggcatc gtcgcgcgcg cctcccgtga acctcaaggg
    21841 tgacgcgcgg gtcgccgcgg tgacgttggc gtccgcggtg acggatgggg ccaccccgtg
    21901 cgagaatccc ggcgcatgac cggaccggac gacaatctcc ccggagcgcc tctggcgcag
    21961 ccgccgcacg gcttctggcc cacggtgcgc cgcgacctgc gcctcggagt catcgccggg
    22021 ctgcagacct tctgggacct cgccaaggtc atgatcccgg cctacggcgt cacgctcgcc
    22081 ctggagcagc tcggtgtgat cgggcggctg gcggacgtcg cgcggccgct catgcaggcc
    22141 ctgggcctgc ccggggacgc ggccgtcccg ctcgtcgtcg gctacgtgct caacatctac
    22201 gccgccgtcg gcgcgatgca ggcgctggat ctcacgaccg gccaggtcac cgtcatcgcc
    22261 atcgccatcc tcatcggcca caacctggtg gtcgagggcg ccgtgctcca caaggcgggg
    22321 atgaacggct tcgccttcgg cgccctgcgc atcgtcgccg ggctcgtggc ggccggcgtc
    22381 gccaacctgc tgatggggct ggtctgatgt ccgccttcct ccgcgagttc acgaccgggt
    22441 cgctcgacct gctgctgcgc gtgttcctca tcgtggtgcc catcatggtg gtgctcgagc
    22501 tcttcgaagg cacgcgtgcg ttccgcgccg tggtgcgcgc gtgggccagg ctggtccgtc
    22561 acgtcggcct cgacgagcgc tccgcggcgc ccacgctcgt gggcttcctc ttcggcctcg
    22621 cctatggcgg cggcgtgatc gtccgcgacg tgcgccgcca cgacctcggc cgccgtcagg
    22681 tcttcatcat gagcgtcttc ctcagcatgg tgcacgccat cgtcgaggac tccctcatct
    22741 tcatcgctct gggcgcgagc gtcttctggg tcgtcggcta ccgcatcctg tgggcggccc
    22801 tggtcacgct ggcgctcacg gcgctggccg gcttctggct gcgccgccgc cgagggtccg
    22861 ggagtctcgg cggctgagat cgggaccggc cggcggccgc cgtcgcggtc gccgtcccgg
    22921 gcgggccacg ttgacgaggg ccgcccagtt ccgtaatctc gactggattt atcgggaatc
    22981 tgcagcgagg agcttgacgc aggtgaacat ctccatgaag agcagatacg cggtcagggc
    23041 cctcacggag ctcgcccgcg tggaacagac gcagctgggc aagcccgtgc gtctcggcga
    23101 gatcgccgag cgccgcgcca tgccgctcca gttcctggag caggtcttcg cggcgcttcg
    23161 ccggggaggc atcgtccaca gccgccgggg cgccgcgggc ggctacgccc tcgcccgccc
    23221 tgccgacgac atcaccgtcc tcgacgtggt cgccgccctc gacggggtgc cgtctccggc
    23281 cgagtgcacg cagggccgct gcgaccaggt ggaccgatgc ggtgcgtcct cggtctggat
    23341 cgacgcgcag cacgcgctcg agcgggtgct cacggccacc accatcggcg acctgctgcg
    23401 gcgcgaggac gcgctgcgcg agtcggcgcc gatgtaccac atctgacccc gggggagagg
    23461 gcatgaagag ggcaggcaac atcaccgagc tcatcgggcg cacgccgctg gtgcggctca
    23521 accgtatctc cgacgagacc ggggcggagg tgctgggcaa gctcgagtcg ttcaacccgg
    23581 gcgggagcgt caaggaccgc atcgggctgg cgatgatcga cgcggccgag cgtgacggga
    23641 tcatcgcccc cgggcgcagc accctcgtcg agccgaccag cggcaacacc ggcgtcgcgc
    23701 tcgccatggt ggcggcggca cgcggctacg acctggtgct caccatgccc gactcgatga
    23761 gcctggagcg gcgcgcgctg ctcaaggcct acggcgcgcg cctcgtcctc acccccggcg
    23821 ccgaaggcat gaccggcgcc gtggaccgcg ccgtggagct cgcgggcgag ctgggcgacg
    23881 cgttcgtgcc ccagcagttc gagaaccccg ccaacccgga cgtgcacggg cggaccaccg
    23941 ccgaggagat ctgggacgac accggcgggg aggtcgacgt gttcgtcgcc ggggtcggca
    24001 cgggcggcac gctcaccggc gtgggccggg tcctcaagga gaggcgcccc ggcctgctgg
    24061 tcgtcgccgt ggagccttcg gattcgcagg tgctctccgg cggcccggcg ggttcccacc
    24121 gcatccaggg catcggcgcg gggttcgtcc ccaagatcct ggaccaggag ggctacgacg
    24181 aggtcctcac cgtgagcggc gacgacgcgt tcgacgcggc gcgccgcctc gcgtccgagg
    24241 agggggtgct cgtcggcatc tccgccggcg ccaacgcctg ggcggccggc gagctcgcgc
    24301 gccgcgacgg catgcacggc aaggtcgtgg tgacgatcct ctgcgacacg ggagagaggt
    24361 acctctcgac gccgctgttc gcggacctgg cggagccgca ggcgtgagga tcagcgggca
    24421 cgagcgccgg cggtacgact gccagctggt cctgaaggag gtcggcgagc gcggtcagca
    24481 ggcgatccgc gacgctgccg tcctcgtcgt cggcgccggc ggcctcggcg cgcccgtgct
    24541 gtactacctc gccgccgccg gcgtggggcg catcggcatc gtcgacgacg acgtggtcga
    24601 gctctcgaac ctgcagcggc agatcctgtt cacgaccgcc gacatcggcc ggcccaaggc
    24661 ggtggtggcg gcggagcggc tgcgggcgct caaccccgag gtgacgccgg aggcgcacac
    24721 ctcgcgcctg cgcgccgcca cggcgttcgc gctgatcgac gcctacgacg tcgtggtcac
    24781 cgccgtcgac aacttcccga cgcgctacct gctcaacgac gcctgcgtgc tgcgcggcaa
    24841 gacgctggtc gacggcgcgg tcctgcgcat gacggggctc gcgatgacga tcaagggcgg
    24901 ggagaccgcc tgctaccgct gcctgtttcc cgagcctcct ccgcccgccg gcgtcatcag
    24961 ctgctccgag gccggggtgc tcggccccat ccccggcctc atcggatcga tccaggcgct
    25021 cgaggtcctc aaggtcctca ccggcgccgg gcggccgctc tacgaccgcc tgctgcagta
    25081 cgacggcggg gcgatgacgt tcaccgaggt cgaggtctcg cgggtcccgt cgtgcccggt
    25141 gtgcggcgag agcccgacgg tgaccgagct ggtcgacgcc gatgcctgac gaccccgtcc
    25201 tggcgcgcct gctggcgcag ttcgccggcg ttgagcgcgc ctgcgtcgcc ttctccggcg
    25261 gcgccgactc cagcctcgtg cttgcggccg ccgcgcgtgc tctgggccac gggaacgtgg
    25321 tcgcggtcac ggccacctcg cccacgtacc tggacgagga actggcggcc gcccgcgcgc
    25381 tggccgacgg gctgggcgtc gagcacgtgg tcgtcgccac gcacgagttc gacgacgggc
    25441 gcttcgtggc gaactcccgc gagcgctgct acttctgcaa ggcgggcctg ctggacgagc
    25501 tcgtgcgggt cgccgcggcg cacggcgggg ccgccctggt cgacggcgcc aacgccgacg
    25561 acgccggcga ccaccgtccc ggcatgcgcg ccgccgctga gcgcggcgtc ctgcagccgc
    25621 tgctggccgc cggcatcggc aaggccgagg tgcgccgcct ggcgcgcgag ctcggcctgt
    25681 ccacctggga cgcgccgcag caggcgtgcc tggcatcgcg cgtcccgtac ggcgaggcca
    25741 tcaccccggc caagctcgcc gccgtggcgg acgccgagag ggcgctgcgc gcgctcggct
    25801 tccgccagtg ccgcgtgcgc caccacgggg acgtggcccg cctggaggtc gagcgcgacg
    25861 agctcggccg ggcgctcgag ctgcgggagc agatcgcccg cgccgtccac gccgccgggt
    25921 tcacctacgt cgccctggac atggacgggt tccgcacggg aagcatgaac gaggcgtgaa
    25981 tactccacgt agcgaaggta tcgagcgggg cgccgaagaa gcaccgctca tagcgtaccg
    26041 cgggcctcgg cggcactccg ccgtccgagc gcaccgcatg gactgaggag gtgacggccg
    26101 tgatccgcaa gctgttcgcc ctgacgggcg tgagcgtcgc cgcgtgctgg ttcctgggac
    26161 gagggacgcg cggctgtcgc cccgtcaacg aggtcgtcgg agcgctgcgc caggacgtgc
    26221 acacggccat cgaagagatc acgagcgacg tcaaggccgt gcaggagcgc gacccggcgg
    26281 ccacgtccaa ggtcgagatc atcacctcgt actcggggct ccacgccctg tggatgcacc
    26341 ggctcgcgca caagctgagc aacgtgggcg tcccgctcgt gccgcgctgg atcagccagt
    26401 tcaaccgctt cgtcaccggc gtcgagatcc accccggcgc gaagatcggg cacgggctgt
    26461 tcatcgacca cggctccggc gtcgtgatcg gggagaccgc cgagatcggc gacgacgtca
    26521 ccatctacca gggagtgacg ctcggcggca ccggcaagga gaccggcaag cgccacccga
    26581 ccgtgggcga caacgtcgtg ttcggcgccg gcgcccaggt gctggggggc atcaccgtgg
    26641 gcgacaacgc caaggtgggc gcgggcagcg tggtggtcgc cgacgtgccc aagaactcga
    26701 ccgtggtggg aaaccccggc aggccggtcc tgctgcaggg gcagaaggtc ggcatccccg
    26761 acatcgacta ccggcacctc ccggaccccg tggccgaggc gatcaaatgt ctcgtggccc
    26821 gcatcgtgga gatggagaag gagctcgacg agatccaccc ggagcggagc ggcaagcgcc
    26881 agcaggccgt gcgcgagacg caggccgtgc tcgccgagct gctgcggttc gattcgggcg
    26941 ccgggatctg acgccgcgcg gccgcgttcg tcatcccccg gggtgaccgg cgtcccgagg
    27001 ggggtatgct acgattgctg ccctcccagg cgacctgtga acggagcgtg atcaccccat
    27061 gagcgaggtc cggcgactcg tgctcgacgt cctcaagccc caccagccca cgatgctcgt
    27121 gctcgccgac aagctcggcg acctcgaggg catccacggg gtcgacatca cgctgatcga
    27181 gatcgacaac aaggtcgaga acatcaagat cacgctggag ggcgacgacg tcgacttcca
    27241 ggacgtcgcc gaggtcgtgc aggacgccgg cggcagcatc cactccatcg acaaggtctc
    27301 ggccggcaag cgcatcatcg acgaggtcga gaccccgcag gacgccggcg ccagctggct
    27361 tcgctgaccg cgccggcccc taaagccctg gtgagccgat gagccttccg ggcagtccca
    27421 gcatcggggt gcgcgtgcgg cggcagctcg ccacgctgcg ccactatcac gagatcgccg
    27481 aggtcggcga gatcgcccgc cggtacttcg ccatgaacgc gttcgacggc gtgctcacgg
    27541 ccatcggcgt gctcgtgggc ggctatctcg gcggcgtgga cagcccgcgc gccatcttca
    27601 ccgtcgtgct gaccacggcc gtgggcatgg gcgtctccgg cttctacggc tcctacctgg
    27661 tggagcgcgc cgagcgcggc agggccatgc gcgagctgga ggagtccacc ctctccagcc
    27721 tccaggggtc gaccatcgcc tcggcctccc ggtacgccac catcgtcatc gcgttcgtcg
    27781 acggcgcctc gccgttcgcg gccgcgctca tcgtgatgat cccgttcgcg ttcacctccg
    27841 tgatcagcat gcacacggcg tacttcctcg ccgtcgccgt cggcctcgtc gagctcttct
    27901 tcctcggcgt gttcctcggg tccatctcgc gcgagcgcct gtggctctcg gggttgcggc
    27961 tcgtggtcgc cggcgccatc gccctgatca tcagcctcct gctcggcgga ggcgtgggat
    28021 gacggcggac cacccgctgc tcgcgggcct caacgacgag cagcgcaagg cggtcacgca
    28081 cgtcgacggc ccgctgctca tcctcgccgg ggccggctcg ggcaagacgc gcgtgctgac
    28141 gcaccgcgtc gcctggctgc tgcaggtcga gcacgtgcgc ccggacgagg tcgtcgccat
    28201 caccttcacc aacaaggccg ccggcgagat gaaggagcgc atcgagggcc tcgtcgggcc
    28261 ggtcgcgcgc accatgtgga tcagcacgtt ccacagcatg tgcgcgcgcc tgctgcgccg
    28321 cgaggcccag cgcatgggct acaagagcac gttcaccatc cacgacgagg acgaccgccg
    28381 ccggctcatc aagcgctgca tggaggagat caacgtcgac cccaagcgct acccgccgga
    28441 gtcgatcagc cggcagatca gcgacgccaa gaaccagctg ctcggtccgg cccagttccg
    28501 cgagcagggc gggcgcttcg cgcagatcgc cgccgacgtc tacgagctct acgagaagcg
    28561 ccacgccgag atgaacgcca tggacttcga cgacctgctc atgaagaccg tggagctgct
    28621 cgagggctat cccgaccggc tccagcacta ccagcaggcg ttccgcttcg tgctcgtgga
    28681 cgagtaccag gacacgaacc acgcccagta ccgcctcgcc aacctgctcg ccggcgcgca
    28741 cggcaacctc gccgtggtcg gcgacgacga ccagagcatc tactcgtggc gcggcgccga
    28801 catccgcaac atcctcgagt tcgagcgcga ccacccggac gcggaggtca tccggctgga
    28861 gcagaactat cgctccacgc agaagatcct cgacgccgcc aacgccgtgg tcacccacaa
    28921 ccgcaagcgc aagggcaaga acctgtggac cccgaggggg gagggcgcgc tcgtccaggt
    28981 cgtggaggtc aacgacgagc gcgccgaggc ccagttcgtg gccagcgagg tgcagaagct
    29041 gctggagggc gaggcggcgg gagcggagcg agcctaccgg ccagacgagg tggccgtgct
    29101 ctaccgcacc aacgcgcaga gccgcgtcct cgaggagcag ttcgggcgct acgccatcgc
    29161 ctatcaggtc atcggcgggc ccaagttcta cgagcgcgcc gagatccgtg acctggtcgc
    29221 ctatctcacc atcctcgtga atcccgacga cacgcagcgg ctgctgcgca tcgtcaacac
    29281 gcccaagcgc ggcatcggcg cgaccagcct gcagcgcctg caggcgcacg ccgcgagcgt
    29341 gggggagagc ctgtggcaga cgctgcgcgg cgccggcgac gtaccggggc tgtccgccgg
    29401 cgcggtgaac ggcctgctgg ccttcgcccg gacgatggag ggcctgcagg ccacgcacgc
    29461 cggccgcccc gtcgccgagg tcgtgcgcag cctgctcgac gagaccgggt acgaggcggc
    29521 gctcgccgcg cagaagaccc tcgaggccga gggccgcatg gagaacatcg aggagttcgt
    29581 cggcgtggcc gcggagtacg acaagcgcgc cgacgagccc agcctcgacc tcttcctcca
    29641 ggagatctcg ctctacagcg acagcgacgc cctcgccgac accagctcgc tgctcacgct
    29701 catgacgctg cacaacgcca agggcctgga gttcccggtg gtgttcatct ccggcatgga
    29761 ggagggcgtg ttcccgcacc agcggtcgct ggacgagcag aacgtggaag aggagcggcg
    29821 gctcgcgtac gtgggcatca cgcgcgcgat ggaccggctg tacctggtgc acgc
//
LOCUS       DASUMX010000161        19752 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_483568, whole genome shotgun sequence.
ACCESSION   DASUMX010000161 DASUMX010000000
VERSION     DASUMX010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 19752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19752
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483568"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..1068)
                     /locus_tag="VFZ86_08040"
     CDS             complement(70..1068)
                     /locus_tag="VFZ86_08040"
                     /inference="COORDINATES: protein motif:HMM:NF024138.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX5642273.1"
                     /translation="MDEISHIDAIETVQILANADRLRLLRILMRGPATLTQLVDSVGH
                     YPAWVRHHVVTLERAGLVELVEQRKTKGYVEKFYCATARAYAVDFLVLPEEGAHGLLV
                     ILGSDDLALDLLADRLRADEDAPDVVTVAMGSLEGLIALRHGLGQVAGCHLLDLATGD
                     FNRSYARALFPGRRLTLLTLAHRQQGLMLPPGNPRGYGALEEAVADGARIINRNRGSG
                     TRVALDRLLAAAGVEGATVDGYADEVTTHRQAARLVADGSAEVALGIYAAAKAQGLDF
                     VPLLEERYDLVTPTSVYESGLLDLTLALLRGDSFKRAVRSLGGYSTRETGVVTTLV"
     gene            complement(1171..1374)
                     /locus_tag="VFZ86_08045"
     CDS             complement(1171..1374)
                     /locus_tag="VFZ86_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012963954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HEX5642274.1"
                     /translation="MTLSARNQLPGTVKSVVLGSVMAEVVVDVAGNEVAAAVTRHSAE
                     TLDLRAGDSVKVVIKATEVMIDK"
     gene            complement(1674..3197)
                     /locus_tag="VFZ86_08050"
     CDS             complement(1674..3197)
                     /locus_tag="VFZ86_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX5642275.1"
                     /translation="MSTTARDAAGIRVSEIVAPDLVIKGPYGTGEEMTLRAPWDGAEL
                     ARVPTATLEDMDRVLDRARVAAEGFRWTAAWKRAEILERTSHLIEENAEQIARIIAAE
                     GGKPLKDARVEAKRGASTFRWAGEEAKRTAGDLVQMDADPSGEHRFGWTIREPRGVIA
                     AISPFNFPLNLVAHKVAPALAGGNAVVLKPASNTPLTALRLAELLAEAGLPDGTLQVV
                     VGSGRTIGNKLVTDERTNMVSFTGSPPVGRQIASDAGMKMITLELGNNSATIVDEDAN
                     LDLALQRVVAGGFANSGQICISVQRVVVHQAVYDRFMEKLVPAVAALKVGDPLDETTD
                     VASLINEDETERVKGWIQEAVDQGATLATGGVDGAGRLLPTVLTDVTREMKVCAREVF
                     GPVLSILKARDLCEAIDISNDSEMGLQAGVFTNDLNKAMYAARRLEVGGVMINEVPTF
                     RVDHMPYGGIKGSGIGREGLKYAIREMTELKMVAIREMDWQTGGPPVDACLREPLGP"
     gene            complement(3327..4970)
                     /locus_tag="VFZ86_08055"
     CDS             complement(3327..4970)
                     /locus_tag="VFZ86_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX5642276.1"
                     /translation="MATVVPDTEVRRYQMYIDGRWTDAASGETYDVVDPASEKVIARV
                     PKGGVADAEEAVRAAREAFDTGPWPRMKAMERAEVMTRAAALLRERAEDFARLESRQM
                     GKLLEESRVDMLDTAHTFELYAGMVQDQHGETLEVPGETMSMVVREPVGVTVGITPWN
                     YPMLMAAWKAGPSLAAGNVMILKPATLSPLTNLELARVFEEAGLPKGVFQVVTGPGGE
                     IGDHLAGHQDVDMVAFTGSVEVGKHIMRRAAENVKKIGLELGGKSPNIVFADADFEAA
                     IDGALIGIFAGTGEVCSAGSRLLVERSIHDRFVGELVSRAMAIKVGDPLDEESEMGPL
                     VSRQQLDTVEKYVAIGKEEGATLATGGRRLSGSGFYYEPTIFVNVDNSMRIAQEEIFG
                     PVLVVIPFDSEEEAIRIANDTIYGLAGAVWTKDVTRAMRVIKALRAGITWVNTYHPTY
                     SEAPWGGYKQSGIGRELGSYGLDEYTEVKQINIDLTGVPMGVYQRGKEGSGSMGAGLE
                     GASGNGRGKVVPLSDRRPFREGESKKDEAWVEFCENCRP"
     gene            complement(5163..5348)
                     /locus_tag="VFZ86_08060"
     CDS             complement(5163..5348)
                     /locus_tag="VFZ86_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642277.1"
                     /translation="MTPGNIPRLIETLKARAGDDKHIACEDAFQIARDLDVPLADVGR
                     TCNELGIKVMQCQLGCF"
     gene            complement(5409..6062)
                     /locus_tag="VFZ86_08065"
     CDS             complement(5409..6062)
                     /locus_tag="VFZ86_08065"
                     /inference="COORDINATES: protein motif:HMM:NF018766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HEX5642278.1"
                     /translation="MVGLLLAASLVCVGLIVARSWYSHTYHYWFLLWNLVLAWVPFVF
                     AAIAHRLASARRAVLYVLIVAAAIVWLAFFPNAPYILTDFLHLGSMGDIVPGWYDVLM
                     LYWFSWTGLILGIVSLYLMQEIVTRGLGTAAGWVFVVVAAGLGSFGIYLGRFLRWNSW
                     DLVRRPGPLADELLGRVTDPQSQPRLLGFTLLFALLFLFIYAAVYIFAKLTKPTGRP"
     gene            complement(6245..6493)
                     /locus_tag="VFZ86_08070"
     CDS             complement(6245..6493)
                     /locus_tag="VFZ86_08070"
                     /inference="COORDINATES: protein motif:HMM:NF025807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEX5642279.1"
                     /translation="MTGAAGTISVKLFAGLELRARERRTAYELDPRETATVGAVTEAI
                     GLEPGAAGLILVNGVHAAGDRVLAPGDEVALFPPLGGG"
     gene            6580..7560
                     /locus_tag="VFZ86_08075"
     CDS             6580..7560
                     /locus_tag="VFZ86_08075"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX5642280.1"
                     /translation="MTPAENDIPTAPPLEGAPGTAPAGELHSGAAAPVDDRDQALRAV
                     QVALIKEIDTAISRLERARMGESTREHLEILRSLLKAQVTAYLEHFVARRATLFSHTS
                     LMYAGEVGAPGVVSTTFRGLLESGALTGGQGTKLAQLLSDRRTLIVFGDRATGKSTLL
                     NSLFELISVDERFVSIERGADLPALKERSFCVRLGVDRDTDIAALFAKVRRMDPSRLV
                     IGELHAQEVREFFGFLAEDPRTGGLATLRTETVRRAVDGIVAAFGGDPARARDAVARV
                     RPAFAHMHADERGRPRLAAIWSVEGLQDGEIVLREVQTGPPSAAELVAEA"
     gene            7676..8053
                     /gene="rpsF"
                     /locus_tag="VFZ86_08080"
     CDS             7676..8053
                     /gene="rpsF"
                     /locus_tag="VFZ86_08080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEX5642281.1"
                     /translation="MVPVHAAARRLFTPAPASWLGPHRPQEVIGINRYELMLIVNPNA
                     DEDRQKEIVDRLRTNVEKDGGAIAGTDEWGKKKLAYEIKKESEGVYTVITFTATPQTL
                     SEIERVLSITDEVLRFGTKRLKS"
     gene            8082..8564
                     /locus_tag="VFZ86_08085"
     CDS             8082..8564
                     /locus_tag="VFZ86_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006721900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEX5642282.1"
                     /translation="MAGSINSVVIVGNLTRDPELRATPSGTSVCSLRVAVNDRVKDPN
                     TGEWGDKANYFDVDVFGGQGERCAQYLARGRQVAVSGRLRWREWETQDGQKRQAVSIL
                     ADNVQFIGPRESAGGGGGQGGYQQRPAAAGSSLGAEFPPAAPGAPSDDDFTADDDIPF
                     "
     gene            8609..8878
                     /gene="rpsR"
                     /locus_tag="VFZ86_08090"
     CDS             8609..8878
                     /gene="rpsR"
                     /locus_tag="VFZ86_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007617539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEX5642283.1"
                     /translation="MPSSKPKPRREKKGKAGGFGQARRRYCSFCKEKIEEVDYKSYAT
                     LRRMMSDKGKIRSRRITGTCRRHQNQLASSIKFAREMALLPYVSR"
     gene            8889..9335
                     /gene="rplI"
                     /locus_tag="VFZ86_08095"
     CDS             8889..9335
                     /gene="rplI"
                     /locus_tag="VFZ86_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015740088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEX5642284.1"
                     /translation="MEVILLQDVENLGDKGQLANVARGYARNYLIPRKLAEPATPGKI
                     VEFRRREEERKARDVRMAAQAEEFTATLNKTVVTLAAKAGEGDRLFGSITAADIADAI
                     KDARGITVDKRKVLLEEPIKEIGTFMVDVEVAEGFVATVKTIVAPA"
     gene            9768..11147
                     /gene="dnaB"
                     /locus_tag="VFZ86_08100"
     CDS             9768..11147
                     /gene="dnaB"
                     /locus_tag="VFZ86_08100"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013279247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEX5642285.1"
                     /translation="MAVERIEPPASPHLPPHNLEAEQSVLGAMLVNPNALTAVTEVLT
                     ADDFYRDTHRLIYRGVLTLFERGEEVDVVTLSAQLEREGMLERAGGREFVHTLAEFVP
                     AAANAAYYASIVREQSVLRSLIRVGNEIAELGYQHPGDPTDLLDRCEQKVFAIQQQRS
                     VGEFEHIKQVLVRNFEHLATLDGDRGVTGVATGFEGIDRLTGGFQKANLIVLAARPGV
                     GKTSLALNIAHHMATEGHKADGGKHAVAIFSLEMSAEELGERLMCSSARVSSHKLRTG
                     SLSPDDHAKLVQAAGVLEKADIFIDDTAGLNMFELRAKARRLQAKQPLSLVIVDYLQL
                     MAGDGRSDNRQQEVSNISRGIKQLARELSVPVIAVSQLNRSPEARADKEPQLADLRES
                     GAIEQDADMVMFIYEDPSDPASKGVVKVKIAKHRNGPTGLVRLGFVRDYTQFRTLRQT
                     EDYGYGDDE"
     gene            11260..12711
                     /locus_tag="VFZ86_08105"
     CDS             11260..12711
                     /locus_tag="VFZ86_08105"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HEX5642286.1"
                     /translation="MAPVVTRFQTPALDPVALIRALGLEGQAPVVLLDSAGGSPQLAR
                     RHYLAWDPVFRLRARRGAVSVAAGGGVRREDRPWTRGLADQAAALTALAPFEALRQAT
                     RIAALERPVPDLPEAEALVGLLSYDAVRYLERLPDTVRDDLGIPDIDVFLPGRLLRFD
                     LESGEAVLVDRLAADEGAAAPAAARVGEALQRARRPSPAALHGNDLPVFRSTFTQPEY
                     EEVVRRTREYVFAGDIFQANLSQRLDLCYALPSLHLYDTLRTVNPSPFAGYLHFGEYE
                     LISSSPERLVSLDAEGWAETRPIAGTRPRGSLTAEDLTDELNLDPKDRAEHIMLVDLE
                     RNDLGRVCEYGTVRVNELMVNEFYSHVIHIVSNVRGHLHPSRDAVDLVTAMFPGGTIT
                     GCPKVRCMEIVDELETVRRGPYTGSFGWVAARRLDLNIIIRTLVRKGDRLFLQAGGGI
                     VADSVPVREYRETLHKAAGMLRAVSASIAERAG"
     gene            12780..13598
                     /locus_tag="VFZ86_08110"
     CDS             12780..13598
                     /locus_tag="VFZ86_08110"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEX5642287.1"
                     /translation="MLILNGVLMSEAEATLPATDRALTHGLGLYETIKLEDGVPVFFE
                     EHVARLVQGIAALGLDKDIDKPELAEQICRLSEANGRDHNSCRLLVTAGAPDGSPSLL
                     VQTDRRDFPQRPLRVVTYRGVRVSAQYKAMTVMQSTFAQRAAQAAGVDDALLVDGEGR
                     IFEGATSNVFVVRAGGLITPPAEGDILPGVLRAKVEELATSAGIPVVEAWARVADLRP
                     DDGVLLTSSIRGIVPVERVDGVQLLVHEQELTQVRSLVGEAEAASTEAFRAAYL"
     gene            13687..14922
                     /locus_tag="VFZ86_08115"
     CDS             13687..14922
                     /locus_tag="VFZ86_08115"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX5642288.1"
                     /translation="MRRVALIIAVVALVAAIAAFAVTRGPSPGAAGGSTTAPGTNAAP
                     GATTPAADAAGDASVSGTAAAARATTRPPSVRLSQIPGSYSQPVYVTAPRGDTSRLFI
                     VEKTGAIRIVKDGAVLQQPFLDISAQVSRGSEQGLLSMAFHPRYASNGRFYVNYTNAS
                     GDTRVVRYRVSGTDPDRAAASSARVVLRVDQPYANHNGGQLQFGPDGRLWVGMGDGGS
                     GGDPQRRAQDPRSRLGKMLRINVNVSPARVGIYAKGLRNPWRFSFDRATGALWIGDVG
                     QNAWEEVDYLRPGRPAGANFGWNAYEGTHVYDAAVAARLDRSSLTWPVSQYSHSVGYS
                     VTGGYVYRGTAVPSLRGFYVFADFSGRVWAKRGPGADRYALPGADRQLGQIASFGEDA
                     RGELYVVSLAGSVFRIAAP"
     gene            complement(14950..15642)
                     /locus_tag="VFZ86_08120"
     CDS             complement(14950..15642)
                     /locus_tag="VFZ86_08120"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5642289.1"
                     /translation="MPQAALHWERRYAAEERVWGDGPSELARLAVARLRPYATPDVTI
                     LDVGCGYGRDTRYLAGELGCRVVGLDASPAAIAAARKEHATALRRSRSARFDAEYVAG
                     DLGSLASDPEHAGPYDVVFSCNVYHLLGPIGRREFAAAIAAAARPGGLLFLSTLSPRD
                     AEHYAVGEPVSGEERSWVHTVYLHFCTAEELTRDFSAFEISDLEERRYDEPRERGPAH
                     HHTSWFLEGRRR"
     gene            complement(15693..16565)
                     /locus_tag="VFZ86_08125"
     CDS             complement(15693..16565)
                     /locus_tag="VFZ86_08125"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005224675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEX5642290.1"
                     /translation="MTTLYESNESAWELISRGKVRDVYAYAPNQMLIVTSDRLSAFDV
                     ILPTPIPDKGVVLTQIANFWFEQTDRIIPNHLVDPDPFSTDAGHRHLAGRSIVVHRAE
                     ALPVEAIVRGYLTGSGRKDYEKTGAVCGIELPEGLVEASRLPEPIYTPSTKAAIGDHD
                     ENVSFDKTVDLIGREMAEKVRDMALELYTFGAEYALARGIIIADTKFEFGVLDGELIL
                     IDEIMTPDSSRFWPADGYEAGKSQPSYDKQYVRDYLETLDWDKTAPGPELPPEVAART
                     SEKYLEAWRRLTQS"
     gene            complement(16603..18438)
                     /locus_tag="VFZ86_08130"
     CDS             complement(16603..18438)
                     /locus_tag="VFZ86_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HEX5642291.1"
                     /translation="MSLQRLDLDDRQAVMYSEGSVCAGTQELVESVTFARVLGLYVDH
                     LEAHNPQALDELGLSGPARAGLEAPAGGDGDPRRARLLDLIRLLANNPLERVSITACA
                     PGDLLARREQLHEFVEGLYDFWRRWDRFLVVRAEVAPAPGDKRPLRGFTETVESLARL
                     VRALYRDVAENITGTRPRVLRQVAAGAEVGVYAVRHKWPVPARYRELLTGIPFVRDLL
                     LYPPLLLDPPMNTRTGQFAETAENPLDGLKLEREEWLCYPALVGRTTVFVYFHRRFMG
                     LGLSLANLFEVASDEEIAAGPDAVYLYGVPPAALARFGDLPTVFHDDRRGRLLVAAVP
                     LEDRFGYFGYLKKMVLTLHNVAAMDRGVMPFHGAYARLTLDDGSRATVLLIGDTATGK
                     SETLEALRVAGEDRIREVRIIADDMGSLEVGPDGRVLGFGSEIGAFVRLDDLQQGYAF
                     GQLDRSIIMSPNKVNARVVLPVTSLEDVLRGYPVDFLLYANNHETVDAEHPVVDRLKG
                     EEAALAVFREGAAMSKGTTTSTGITNSYFANPFGPAQRREQHEALAAATFAAAFRAGV
                     FVGQLRTQLGLPGHESAGPRAAAQALLDLIAAKREGGADGVPRCE"
     gene            complement(18743..19147)
                     /gene="mscL"
                     /locus_tag="VFZ86_08135"
     CDS             complement(18743..19147)
                     /gene="mscL"
                     /locus_tag="VFZ86_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEX5642292.1"
                     /translation="MFKGFRDFIMRGNVVDLAVAVVIGAAFGAVITSFVNDVLMQIIA
                     AVGGQPDFSSLSFKIGEGVIKYGSFLNALITFLLIAAAVYFVVVLPMNKLAERRAKGQ
                     DPTTRECPHCLSEIPVGATRCPHCTSELAAAS"
BASE COUNT         2909 a         7133 c         6858 g         2852 t
ORIGIN      
        1 ctccctatga ggtgctcagg gagaagctgg tgtcgtgacg accgtcatcc gaagtcgtga
       61 ggggcacaat tagacgagcg tcgtgaccac cccggtctcg cgggtcgagt agccgccgag
      121 ggagcggacg gcgcgcttga agctgtcgcc tcgcagcagc gccagcgtca ggtcgagcag
      181 gccgctctcg tagaccgacg ttggcgtcac gaggtcgtac cgttcctcga ggaggggtac
      241 gaagtcgagg ccttgtgcct tggcggcggc gtagatgccc agagctactt ccgcggaccc
      301 gtcggccacc agtcgcgcgg cctgtcgatg agtcgtgacc tcgtcggcgt agccgtcgac
      361 cgtcgcgccc tcgactccgg ccgcggcaag caaccggtcc agcgcgaccc gcgtgccgga
      421 gccgcggttg cggttgatga tgcgcgctcc gtcggcgacc gcctcttcga gcgcgccgta
      481 gccccgcggg tttccgggag ggagcatcaa cccctgctgc cgatgcgcga gggtcaggag
      541 cgtgagccgg cgtcccggga agagcgcgcg tgcgtaggag cggttgaaat ccccggtggc
      601 gagatccagc aggtggcacc ctgccacctg cccgagccca tggcgcagcg cgatgagtcc
      661 ctcgaggctg cccatggcga cggtcaccac gtccggcgcg tcctcgtcgg cccggagccg
      721 gtcggcgagc agatcgaggg ccagatcgtc gctgccgagg atgaccagca gtccgtgggc
      781 gccctcctcg ggcagcacca ggaagtccac cgcgtaggca cgcgcggtgg cgcagtagaa
      841 cttctccacg taccccttgg tcttgcgctg ctccaccagc tcgacgagac ccgcgcgctc
      901 cagcgtcacc acgtgatggc ggacccaggc ggggtagtga ccgacgctgt cgacgagctg
      961 cgtgagcgtg gcggggccac gcatgaggat gcgcagcagg cgcaggcggt ccgcgttcgc
     1021 gagtatctgc accgtctcga tggcatcgat gtggctgatc tcgtccacgg tcgcagcgtg
     1081 tcaagcgccg ccgcaggtgt caacgcaccg gaggcgtgga caggatgtct gtccacgcct
     1141 ccggccctcc gccgctcccg ggggagcctg ctacttgtcg atcatcacct cggtcgcctt
     1201 gatgacgacc ttcacgctgt cgccggccct gaggtcgagc gtctcggccg agtgccgcgt
     1261 cacggccgcg gccacctcgt tgccggcgac gtcgacgacg acctcggcca tcaccgatcc
     1321 gaggacgacg ctcttgaccg tgcctgggag ctgattgcgt gcgctcagcg tcatcgctgc
     1381 ctcctggtcc gcgcccgtca gccgcccgct gcggtgacgt tctcctgttc cctacccggc
     1441 cttctgcgct gaagaacacc atagtaggag aaacgaaaga gggcggcggg cccggaaggc
     1501 ccgccgcccc atgtgctcgg cgcggcgcgc tattgcaccg catgcggggc gtctagcgcg
     1561 ccgcacctcg taagtcagtc aatgtcgcat gcctcctttc ctcgtggctc ccggccgtgg
     1621 cgcgtacagg gaagactctc cccgcactcc ggccgcttgc ccgtccgagc ccctcaaggt
     1681 ccgagcggct cgcgcaggca ggcgtccacc ggtggcccgc cggtctgcca gtccatctcg
     1741 cggatggcga ccatcttcag ctccgtcatt tccctgatgg cgtatttcaa cccttcacgg
     1801 cctatgccgg agcccttgat accaccatac ggcatgtggt caaccctgaa cgtggggacc
     1861 tcgttgatca tgacgccccc gacctcgagc cgccgggcgg cgtacatcgc cttgttgagg
     1921 tcgttggtga acacgccggc ctgcaggccc atctcgctgt cgttgctgat gtcgatcgcc
     1981 tcgcagaggt cgcgcgcctt gaggatgctc agcaccggcc cgaagacctc gcgtgcgcag
     2041 accttcatct cgcgcgtcac gtcggtcagc acggtcggca gcaggcggcc ggcgccgtcg
     2101 acgccgccgg tggcgagcgt cgcgccctgg tccacggcct cctggatcca gcccttgacc
     2161 cgctcggtct cgtcctcatt gatgagcgat gccacgtcgg tcgtctcgtc cagagggtcg
     2221 ccgaccttga gcgccgccac cgcggggacg agcttctcca tgaaccggtc gtacacggcc
     2281 tggtgcacga cgacgcgctg caccgagatg cagatctggc cggagttggc gaacccgccg
     2341 gccacgaccc gctgcagggc gaggtcgagg ttggcgtcct cgtcgacgat ggtcgccgag
     2401 ttgttgccga gctccagggt gatcatcttc atcccggcgt cgctggcgat ctgcctgccg
     2461 accggcgggc tgccggtgaa gctcaccatg ttggtgcgtt cgtcggtgac gagcttgttg
     2521 ccgatggtcc ggccggagcc gacgacgacc tggagcgtgc cgtcggggag gccggcctcg
     2581 gccagcagct ccgccagccg cagcgcggtg agcggggtgt tggaggccgg cttgaggacg
     2641 acggcgttgc cgccggccag cgccggcgcg accttgtgcg ccaccaggtt gagcgggaag
     2701 ttgaacggcg agatggcggc gatcacgccg cgcggctcgc ggatggtcca gccgaagcgg
     2761 tgctcgccgc tcgggtcggc gtccatctgc acgaggtcgc cggccgtccg tttggcctct
     2821 tcaccggccc agcggaaggt gctggcgccg cgcttggcct ccacgcgcgc gtccttgagc
     2881 ggtttgccgc cctcggccgc gatgatgcgc gcgatctgct cggcgttctc ctcgatgagg
     2941 tgcgaggtgc gctcgaggat ctccgcgcgc ttccaggccg cggtccagcg gaacccctcg
     3001 gccgcgacgc gcgcgcggtc gagcacgcgg tccatgtcct cgagcgtggc cgtcgggacg
     3061 cgcgccagct ctgcgccgtc ccaaggggcg cgcagcgtca tctcttcgcc ggtgccgtac
     3121 ggccctttga tcacgaggtc cggggcgacg atctcgctga cccggatgcc cgccgcgtct
     3181 cgtgcggtgg tactcatttc ctccccctcc tccgacgtac gccggcggcg actggtgtcc
     3241 ctgccgccgt gcttacccgg acgggccggc cggctcacgc gccgccggtg gcggcgcctc
     3301 ctccggccgg cccggcactc gggcggtcac ggccggcagt tctcgcagaa ctcgacccag
     3361 gcctcgtcct tcttgctctc gccttctcgg aacggtcgcc ggtcgctcag ggggacgacc
     3421 ttgccgcggc cgttgccgga ggcgccctcc agaccggcgc ccatcgaacc ggagccctcc
     3481 ttgccgcgct ggtacacgcc catcggcacg ccggtgaggt cgatgttgat ctgcttgacc
     3541 tccgtgtact cgtcgaggcc gtagctgccc agctcgcgcc cgatgccgct ctgcttgtag
     3601 ccgccccacg gggcctcact gtacgtcggg tggtacgtgt tgacccaggt gatgccggcg
     3661 cgcagggcct tgatcacccg catcgcgcgc gtgacgtcct tggtccacac cgccccggcc
     3721 agtccgtaga tcgtgtcgtt ggcgatcctg atcgcctctt cctcgctgtc gaaggggatg
     3781 acgacgagca ccggcccgaa gatctcctcc tgcgcgatgc gcatggagtt gtccacgttc
     3841 acgaagatgg tgggctcgta gtagaagccg ctgccgctga ggcggcggcc accggtggcg
     3901 agcgtcgcgc cttcttcctt gccgatcgcg acgtacttct ccaccgtgtc gagctgctgc
     3961 ctgctcacca gcgggcccat ctcgctctct tcgtcgagcg ggtcgccgac cttgatcgcc
     4021 atcgcgcggc tgacgagctc gccgacgaag cggtcgtgga tggagcgttc gacgagcagg
     4081 cgcgagccgg ccgaacagac ctcgcccgtg ccggcgaaga tgccgatgag agctccgtcg
     4141 atggccgcct cgaagtcggc gtcggcgaac acgatgttgg ggctcttgcc gccgagctcc
     4201 agaccgatct tcttcacgtt ctcggcggcc ctgcgcatga tgtgcttgcc gacctcgacg
     4261 gagccggtga aggccaccat gtcgacgtcc tgatgcccgg cgaggtggtc gccgatctcg
     4321 ccgcccgggc cggtgaccac ctggaagacg cccttgggca gaccggcctc ctcgaagacc
     4381 ctggcgagct cgaggttggt gagcgggctc agggtggccg gcttgaggat catcacattg
     4441 ccggccgcca gcgacggccc cgccttccac gccgccatga gcatcgggta gttccacggc
     4501 gtgatgccga cggtgacgcc gacgggctcg cgtaccacca tgctcatggt ctcgcccggt
     4561 acctccagcg tctcgccgtg ctggtcctgg accatgccgg cgtacagctc gaaggtgtgc
     4621 gcggtgtcga gcatgtccac gcgcgactcc tcgagcagct tgcccatctg gcggctctcg
     4681 agccgggcga agtcctccgc gcgctcgcgc aggagcgcgg ccgcgcgcgt catgacctcc
     4741 gcgcgctcca tcgccttcat cctcggccag ggaccggtgt cgaacgcctc gcgcgcggcg
     4801 cggacagcct cctcggcgtc cgcgacgccg cccttaggga cgcgcgcgat caccttctcg
     4861 gacgcgggat cgaccacgtc gtaggtctcg ccgctggccg catcggtcca gcggccgtcg
     4921 atgtacatct gataacgcct gacctctgtg tcgggtacca cggtcgccat gcttctcacc
     4981 tcacgggcgc ccggcgggcg ccgggcgcgg ttcgttgtgg ctgcggcggc ggcgcaggcg
     5041 tgcgccgctg ccgcgaaaag ctcatagcag agcaattccc atgatttgca cagggttaac
     5101 tcgggatgcg ggccgtgcga aagagaggcg agcgcggcag cgctcgcctc agcggcggtc
     5161 gctcagaagc agccgagctg gcactgcatc accttgatgc ccagctcgtt gcaggtgcgg
     5221 ccgacgtccg cgagcgggac gtcgaggtcg cgagcgatct ggaacgcgtc ctcgcaggcg
     5281 atgtgcttgt cgtcgccggc gcgcgccttc agggtctcga tgagtcgcgg gatgttgccg
     5341 ggggtcatgg gccgcagtgt acgcaccgcg agaaggggcg gcaagcgcgg agggcccgtc
     5401 aggtgcgctc agggccggcc ggtgggcttg gtgagcttgg cgaagatgta caccgccgcg
     5461 tagatgaaca ggaacagcag cgcgaacagc agtgtgaagc cgagcagccg tggctgcgat
     5521 tgcgggtcgg tgacccgtcc gaggagctcg tcggcgagcg ggcccggccg gcgcaccagg
     5581 tcccagctgt tccagcgcag gaaccggccg aggtagatgc cgaagctgcc gagaccggcg
     5641 gcgaccacga cgaagaccca gccggccgcc gtgcccaggc cccgggtgac gatctcctgc
     5701 atgagataga gagagacgat cccgaggatg agcccggtcc aggagaacca gtagagcatc
     5761 agcacgtcgt accagccggg cacgatgtcg cccatgctgc cgaggtggag gaagtcggtg
     5821 aggatgtacg gcgcgttggg gaagaaggcc agccacacga tggccgcggc gacgatgagg
     5881 acgtagagca cggcgcggcg cgccgaggcc aggcggtggg cgatggcggc gaacacgaac
     5941 ggcacccacg ccagcacgag gttccagagc aggaaccagt agtggtacgt gtgcgagtac
     6001 cacgagcgcg ccacgatgag gcccacgcag accagcgagg cggcgaggag cagaccgacc
     6061 atggcgtacg cgaaccggtg acgtcccggg aggcgcgtgc ggagccggac gtccggagcg
     6121 gacggcggag cggggtccgc cggcggccgg gacggacccg gcggacggcc ctccggcgga
     6181 cggccctccg gcgcccgccg cacggcgccg tcgaaggggt ccggctcccg cggcccggag
     6241 gagctcaccc gccccccagc ggcgggaaca gcgcgacctc gtcgccgggg gcgaggaccc
     6301 ggtcgccggc ggcgtgcacg ccgttgacga ggatgaggcc ggcggcgccg ggctcgaggc
     6361 cgatggcctc ggtgacggcg cccaccgtgg cggtctcccg cggatcgagc tcgtaggccg
     6421 tgcgccgctc gcgggcgcgc agttcgaggc ccgcaaagag cttcacgctg atcgtgcccg
     6481 ccgcgccggt catgtcccga cgataccact catcccgggc gcccaccctg ccgatacggg
     6541 aggcgagcgc agcgggctgc gacctgcgcg tgaacggaca tgacccccgc cgagaacgac
     6601 atccccactg cgccgcccct ggagggagcg ccgggcacgg cccctgccgg cgagctccac
     6661 agcggcgcgg ccgcgccggt ggacgaccgc gaccaggcgc tgcgcgccgt ccaggtcgcg
     6721 ctcatcaaag agatcgacac ggcgatcagc cggctcgagc gcgcgcgcat gggcgagagc
     6781 acgcgcgagc acctcgagat cctgcgcagc ctgctcaagg cgcaggtgac cgcgtacctg
     6841 gagcacttcg tcgcgcggcg cgccacgctg ttctcgcaca cgtcactgat gtacgccggc
     6901 gaggtcggcg cgcccggcgt ggtctcgacc accttccggg gtctgctcga gagcggcgcg
     6961 ctcacgggcg gccagggcac gaaactggcc cagctcctga gcgaccggcg cacgctcatc
     7021 gtcttcggtg accgcgccac cggcaagtcg accctgctca actcgctctt cgagctcatc
     7081 tcggtggacg agcgcttcgt ctccatcgag cgcggagcgg accttccggc gctcaaggag
     7141 cggtcgttct gcgtgcgcct tggcgtcgac cgcgacaccg acatcgcggc gctcttcgcc
     7201 aaggtgcggc gcatggaccc cagccgcctg gtgatcggcg aactgcacgc ccaggaggtc
     7261 cgcgagttct tcggcttcct ggccgaggac ccccggaccg gcggcctcgc gaccctgcgc
     7321 accgagaccg tgcgccgcgc cgtcgacggc atcgtcgcgg ccttcggcgg cgacccggcg
     7381 cgcgcgcgcg acgccgtcgc ccgggtccgg cccgccttcg cgcacatgca tgcggacgag
     7441 aggggccggc cgcgcctcgc cgccatctgg agcgtcgagg ggctgcagga cggcgagatc
     7501 gtgctccgtg aggtgcagac cggcccgccg tccgccgccg agctggtcgc cgaagcctga
     7561 agcgggcgtc gccgcggccc catcgtcccg ccatcgtcat ccgtcgccgc cccgcgtcac
     7621 cctttccgcc gtccggccgt cgcgctacgg ttgcccctgc gaggtctggg ctggtatggt
     7681 gcccgttcac gccgccgcaa ggcgactgtt cacccctgct ccagcatcct ggctggggcc
     7741 gcacagacca caggaggtga ttgggataaa caggtatgag ctcatgctca tcgtgaaccc
     7801 gaacgcggac gaggatcgcc agaaggagat cgtcgaccgg cttcgcacca acgtcgagaa
     7861 ggacggcggc gccatcgccg gcaccgacga gtggggcaag aagaagctgg cctacgagat
     7921 caagaaggaa tccgaaggcg tctacaccgt catcacgttc acggcgacac cccagactct
     7981 cagcgagatc gaacgcgtgc tgagcatcac cgacgaggtc ctgcgtttcg ggaccaagcg
     8041 gctgaagtcc tagcggcacg accgagggaa gggagtccac cgtggccggg tccatcaaca
     8101 gcgtcgtcat cgtcggcaac ctcacgcgcg accccgagtt gcgggcgacg cccagcggca
     8161 ccagcgtgtg cagcctccgc gtcgccgtca acgatcgcgt caaagacccg aacaccggcg
     8221 agtggggcga caaggccaac tacttcgacg tcgatgtctt cggcggccag ggcgagcgct
     8281 gcgcgcagta cctcgcccgc gggcgccagg tcgcggtcag cggccggctc cgctggcgcg
     8341 agtgggagac ccaggacggc cagaagcgtc aggcggtctc catcctggcc gacaacgtgc
     8401 agttcatcgg cccgcgcgag agcgcgggcg gtggcggcgg ccagggcggc taccagcagc
     8461 ggcccgcggc cgccggctct tcgctcgggg cggaattccc gcccgcggcc cccggcgcgc
     8521 ccagcgacga cgacttcacc gccgacgacg acatcccgtt ctgagcacga gagtgcgcct
     8581 tcgggcgctg taaggagaaa gaacacgcat gcccagctcc aagcccaagc cgcggcgcga
     8641 gaagaaaggt aaggccggag ggttcggcca ggctcgccgc cgctactgct ccttctgcaa
     8701 ggagaagatc gaagaggtcg actacaagag ctacgcgacc cttcgtcgca tgatgtccga
     8761 caaaggcaag atccgctcgc gccgcatcac cggtacgtgt cgccgccacc agaaccagct
     8821 ggcgtcgtcc atcaagttcg cgcgcgagat ggccttgctg ccctacgtga gtcgctgagg
     8881 aggacagcat ggaggtcatc ctgctccagg acgtcgagaa cctcggcgac aaaggccagc
     8941 tggccaacgt cgcccgcggc tacgcccgca actacctgat accgcgcaag ctggcggagc
     9001 ccgcgacgcc gggcaagatc gtcgagttcc gacgtcgtga agaagagcgc aaggcccgcg
     9061 acgtgcgcat ggccgcgcag gccgaggagt tcacggccac gctcaacaag accgtcgtca
     9121 cgctggccgc caaggccggc gagggcgacc gcctgttcgg cagcatcacc gccgccgaca
     9181 tcgccgacgc catcaaggac gcgcgcggca tcaccgtcga caagcgcaag gtcctgctgg
     9241 aggagccgat caaggagatc ggcaccttca tggtcgacgt cgaggtcgcc gagggcttcg
     9301 tcgccaccgt caagaccatc gtcgcgcccg cctgacgacg cgcgactgaa gaaccaggac
     9361 ctgaagcccc gggcgaggcg cgcagagcgc ttctcccggg gcttcgtgct gctgcccaga
     9421 gcggcttcct cgcgtcatgg ctccccgtcg aggggacttc ccatcctcaa ggcctctcgg
     9481 agagcaatcc gaggaggtgg cgggcgatcg tctccggcgc gaaggcatcg acgcgcctca
     9541 ccgctggcgc ctgccacggc gtcggtcgga cggtgccgag ccgtcggaat gggctggtgc
     9601 gcgtgcgcta ctctcaccct ctacttgacg gtgattgtta cccttcctca cgctcggagc
     9661 cgacctccag caggcgccgg acccgtcccg tccgtgcgct atcatcgtcc ggccgagtcg
     9721 catagactcc gagacccacg ccaccaccga gccgcccctg gagagccatg gccgtcgagc
     9781 gcatcgagcc acccgcaagc ccgcacctgc cgccgcacaa cctggaggcg gagcagtcgg
     9841 tgctcggcgc gatgctggtc aaccccaacg ccctcacggc ggtcaccgag gtgctcaccg
     9901 cggacgattt ctaccgcgac acgcaccggc tcatctaccg cggcgtgctc accctgttcg
     9961 agcgcggcga ggaggtcgac gtcgtcactc tgagcgccca gctcgagcgt gagggcatgc
    10021 tcgagcgcgc cggcggacgc gagttcgtgc acacgctggc ggagttcgtg ccggccgccg
    10081 ccaacgcggc gtactacgcg agcatcgtgc gcgagcagag cgtgctgcgc tcgctcatcc
    10141 gcgtgggcaa cgagatcgcc gagctcggct accagcaccc cggcgatccc accgacctgc
    10201 tcgaccgctg cgagcagaag gtcttcgcca tccagcagca gcgcagcgtc ggcgagttcg
    10261 agcacatcaa gcaggtgctc gtacgcaact tcgagcacct ggccaccctc gacggcgacc
    10321 gcggcgtcac cggcgtcgcc accgggttcg agggcatcga ccggctcacc ggcggcttcc
    10381 agaaggccaa cctcatcgtg ctcgccgccc gcccgggcgt gggcaagacg agtctcgcgc
    10441 tcaacatcgc ccaccacatg gcgaccgagg gccacaaggc cgacggcggc aagcacgccg
    10501 tcgccatctt cagcctcgag atgagcgccg aggagctggg cgagcgcctc atgtgctcct
    10561 cggcgcgcgt cagctcccac aagctccgca ccggcagcct ctctccggac gaccacgcca
    10621 agctggtgca ggcggccggc gtgctcgaga aggccgacat cttcatcgac gacaccgccg
    10681 gcctcaacat gttcgagctc cgcgccaagg cgcgccgcct gcaggccaag cagccgctca
    10741 gcctcgtcat cgtcgactac ctccagctca tggccggcga cggccgctcg gacaaccgcc
    10801 agcaggaggt cagcaacatc tcgcgcggca tcaaacagct ggcgcgcgaa ctctccgtgc
    10861 cggtcatcgc agtctcgcag ctcaaccgtt ccccggaggc gcgcgccgac aaggagccgc
    10921 agctcgcgga cctgcgcgag tcgggcgcca tcgagcagga cgccgacatg gtcatgttca
    10981 tctacgaaga ccccagcgac ccggcctcca agggtgtcgt gaaggtcaag atcgccaagc
    11041 accgcaacgg ccccaccggg ctggtgcggc tcggcttcgt gcgcgactac acgcagttcc
    11101 gcacgctgcg ccagaccgag gactacggat acggcgacga cgaatgatca gcgtagacga
    11161 atcatcgagc ggggcgccgc aggggcaccg ctccatgatc gccgcagacg aatcatcgag
    11221 cggtgcgcag taggctcacc gctctgtaga ctggggcaca tggcccccgt cgtcaccagg
    11281 ttccagacac cggcgctcga ccccgtcgcg ctcatccgcg cgctcgggct cgaggggcag
    11341 gcgccggtcg tgctgctcga ctcggccggc gggtcgccgc agctcgctcg ccggcactac
    11401 ctcgcctggg accccgtctt tcgcctgcgc gcccggcgtg gcgccgtgag cgtcgccgcg
    11461 ggcggcggcg tccgccgcga ggatcggccg tggacccgcg gcctcgcgga ccaggccgcc
    11521 gccctcaccg ccctcgcgcc cttcgaggcg ctgcggcaag ccacgaggat cgcggcgctg
    11581 gagcggcccg tcccggacct gcccgaggcc gaggcgctgg tggggcttct gagctacgac
    11641 gcggtgcgct acctcgagcg cctgccggac accgtgcgcg acgacctcgg catccccgac
    11701 atcgacgtgt tcctgccggg caggctcctg cgcttcgacc tcgagagcgg cgaggccgtg
    11761 ctcgtcgacc gtctggccgc ggacgagggc gcggcggcgc ccgccgcggc gcgcgtgggc
    11821 gaagccctcc agcgcgcgcg ccgcccctct cccgccgcgc tccacggcaa cgacctgccg
    11881 gtgttccgca gcaccttcac gcagccggag tacgaggagg tcgtgcgccg gacccgcgag
    11941 tacgtcttcg ccggtgacat cttccaggcg aacctcagcc agcggctcga cctctgctac
    12001 gcgctgccga gcctgcacct ctacgacacg ctgcgcacgg tgaaccccag ccccttcgcc
    12061 ggctaccttc acttcggcga gtacgagctg atcagctcct cgccggagcg cctcgtctcg
    12121 ctcgacgccg agggctgggc cgagacccgc cccatcgccg gcacccggcc ccggggctcg
    12181 ctcaccgcgg aggacctcac cgacgagctc aacctcgacc ccaaggaccg cgccgagcac
    12241 atcatgctgg tcgacctcga gcgcaacgac ctcggccggg tgtgcgagta cggcaccgtt
    12301 cgcgtgaacg agctcatggt caacgagttc tacagccacg tgatccacat cgtgagcaac
    12361 gtgcgcggcc acctgcaccc gtcgcgggac gcggtggacc tggtcacggc gatgttcccc
    12421 ggcggcacca tcaccggctg ccccaaggta cgctgcatgg agatcgtcga cgagctggag
    12481 acggtgcgcc gcgggccgta cacggggtcc ttcgggtggg tcgcggcgag gcggctggac
    12541 ctcaacatca tcatccggac gctggtacgc aagggcgacc ggctgttcct gcaggccggc
    12601 ggcggcatcg tcgccgactc ggtgccggtg cgcgagtacc gggagacgct gcacaaggcc
    12661 gccggcatgc tgcgcgcggt gagcgcgagc atcgccgaac gggcggggtg agccgccgcc
    12721 gatgtgacag cccggcgaat gcgccggcga caccccggct gtagactctc gccgtgacga
    12781 tgctgatcct caacggcgtg ctcatgagcg aggccgaggc caccctgccg gccaccgacc
    12841 gcgcgctcac gcacggcctc gggctctacg agacgatcaa gctcgaggac ggcgtaccgg
    12901 tgttcttcga ggagcacgtc gcccggctcg tccagggcat cgccgcgctc gggctcgaca
    12961 aggacatcga caagcccgag ctcgccgagc agatctgccg cctcagcgag gccaacggcc
    13021 gcgaccacaa ctcctgccgc ctgctggtca cggccggggc gccggacggc agcccgtctc
    13081 tgctcgtcca gaccgaccgg cgcgacttcc cgcagcgccc cctgcgcgtc gtgacctacc
    13141 gcggcgtgcg cgtcagcgcc cagtacaagg cgatgacggt gatgcagtcc accttcgccc
    13201 agcgggcggc gcaggccgcc ggcgtggacg acgccctgct cgtggacggc gagggcagga
    13261 tcttcgaggg cgcgacgtcc aacgtgttcg tcgtgcgcgc cggcggcctc atcacgccgc
    13321 cggccgaggg ggacatcctg cccggggtgc tgcgcgccaa ggtcgaggag ctcgccacgt
    13381 ccgccggcat ccccgtcgtg gaggcgtggg cccgcgtcgc cgacctgcgc ccggacgacg
    13441 gcgtcctgct caccagcagc atccgcggca tcgtgcccgt cgagcgcgtc gacggcgtgc
    13501 agctgctggt gcacgagcag gagctgacgc aggtgcgctc gctggtcggc gaggccgagg
    13561 ccgcgagcac cgaagccttc cgcgcggcct acctctagcc cgtcccgccg cgccggcgga
    13621 ggtcggcggg cgaggccgtc ccggctcagg ggttacgtgc gtcgctccgc gcggaatcat
    13681 cccgccatgc gccgcgtggc cctcatcatc gcggtcgtgg ccctcgtggc cgcgatcgcc
    13741 gccttcgccg tcacgagagg gccttctccc ggcgcggccg gcgggtccac gacggcgccg
    13801 ggcactaacg ccgcgccggg ggcgacgacc ccggcggcgg acgccgcggg cgacgcgtcc
    13861 gtgtcgggca ctgcggccgc cgcgcgggcc acgacccggc cgccgtccgt gcggctctcg
    13921 cagatcccgg gctcctactc ccagccggtg tacgtgacgg ctccgcgcgg cgatacctca
    13981 cgcctcttca tcgtcgagaa gacgggcgcg atccggatcg tcaaggacgg cgccgtgctg
    14041 cagcagccgt tcctggacat ctcggcgcag gtctcgcgcg gcagcgagca gggcctgctc
    14101 tcgatggcct tccacccccg gtacgcgagc aacggccgct tctacgtgaa ctacacgaac
    14161 gcgagcggcg acacgcgcgt cgtccgctac cgggtgtcgg ggacggaccc ggacagggcg
    14221 gccgcctcgt cggcgcgcgt cgtgctgcgc gtcgaccagc cctacgccaa tcacaacggc
    14281 gggcagctgc agttcggccc ggacgggcgc ctctgggtcg ggatgggcga cggcggcagc
    14341 ggcggcgacc cccagcgccg cgcccaggac ccccgcagcc ggcttggcaa gatgttgcgc
    14401 atcaacgtca acgtttctcc ggcgcgcgtg ggcatctacg ccaagggcct gcgcaacccg
    14461 tggcgcttct cgttcgaccg tgcgaccggc gccctctgga tcggcgacgt ggggcagaac
    14521 gcctgggagg aggtcgacta cctgcggccg ggccggccgg ccggcgccaa cttcggctgg
    14581 aacgcctacg agggcaccca tgtgtacgac gcggcggtgg ccgccaggct ggaccggtcc
    14641 tcgctgacct ggccggtctc gcagtacagc cacagcgtcg gctactcggt caccggcggc
    14701 tacgtgtacc gcggcacggc agtcccgtcg ctgcgcggct tctacgtgtt cgccgacttc
    14761 tccggccgcg tgtgggcaaa gcgcggcccc ggggcggacc gctacgcgct ccccggggcc
    14821 gaccgccagc tcggccagat cgcgtcgttc ggcgaggacg cgcgcggcga gctgtacgtc
    14881 gtgtcgctcg cggggtcggt cttcaggatc gccgccccct gaagcagcgg gcgccggcga
    14941 gccgcgggct cagcgccggc gcccctccag gaaccagctc gtgtggtggt gcgccgggcc
    15001 gcgttcccgc ggctcgtcgt agcggcgctc ctcgaggtcg ctgatctcga acgcggagaa
    15061 gtcgcgcgtg agctcctccg cagtgcagaa gtgcaggtac accgtgtgga cccagctgcg
    15121 ctcctcgccg gagacgggct cgccgaccgc gtagtgctcg gcgtcccgcg gcgagagcgt
    15181 gctcaggaag aggaggccgc cgggccgggc ggcggcggcg atcgccgccg cgaactcccg
    15241 gcggccgatc ggccccagca ggtggtagac gttgcagctg aagacgacgt cgtaggggcc
    15301 ggcgtgctcc gggtcgctcg cgagcgaccc aaggtcgccg gccacgtact ccgcgtcgaa
    15361 gcgcgccgac cgcgagcggc gcagcgcggt cgcgtgctcc ttgcgcgccg ccgcgatcgc
    15421 cgccggcgac gcgtccaggc ccaccaccct gcagccgagc tctccggcga ggtagcgcgt
    15481 gtcgcggccg tagccgcagc cgacgtcgag gatcgtgacg tccggagtgg catacggacg
    15541 caggcgggcg acggcgaggc gagccagctc gctcggcccg tcgccccaca cgcgctcttc
    15601 ggccgcgtag cgccgctccc agtgcagggc ggcttgcggc acgcttgcct cgtgcccgcc
    15661 gaacggcccg ccccggccgc ctcggccttc gttcacgact gcgtcaggcg gcgccaggcc
    15721 tccaggtact tctcggaggt cctggccgcc acctcgggtg gcagctccgg accgggggcg
    15781 gtcttgtccc agtcgagcgt ctccaggtag tcgcgcacgt actgcttgtc gtagctcggc
    15841 tggctcttgc cggcctcgta gccgtcggcc ggccagaagc gcgacgaatc gggcgtcatg
    15901 atctcgtcga tcaggatcag ctcgccgtcc aggacgccga actcgaactt ggtgtcggcg
    15961 atgatgatcc cgcgcgcaag cgcgtactcg gccccgaacg tgtacagctc cagcgccatg
    16021 tcccgcacct tctccgccat ctcccggccg atcaggtcca ccgtcttgtc gaatgagacg
    16081 ttctcgtcgt ggtcgccgat cgcggccttg gtcgacggtg tgtagatggg ctcgggcaga
    16141 cggctcgcct cgacgaggcc ctccggcagc tcgatgccgc acaccgcgcc ggtcttctcg
    16201 tagtccttac gccccgaccc ggtgaggtac ccgcgcacga tcgcctccac cggcaaggcc
    16261 tccgccctgt gcaccacgat cgagcgcccg gcgaggtgcc tgtgccccgc gtcggtgctg
    16321 aaggggtcgg ggtcgacgag gtggttgggg atgatccggt cggtctgctc gaaccagaag
    16381 ttggcgatct gcgtgaggac gacgcccttg tcggggatcg gcgtcggcag gatgacgtcg
    16441 aaggcggaga gccggtcgct ggtgacgatc agcatctggt tgggcgcgta cgcgtacacg
    16501 tcgcgcacct tgccccggct gatgagctcc caggcggatt cgttggactc gtacagcgtc
    16561 gtcatgatcg gctcgctcct ctcgctcgct ttgacagcca cgctactcgc atcgcggaac
    16621 gccgtcagct ccgccctctc gtttcgcggc gatgaggtcg agaagagctt gagcggcggc
    16681 gcgggggccg gcggattcgt gcccgggcag cccgagctgc gtgcgcagct gcccgacgaa
    16741 cacgccggcc cggaacgccg ccgcgaacgt cgccgcggcc agcgcctcgt gctgctcgcg
    16801 gcgctgcgcc gggccgaacg ggttggcgaa gtagctgttg gtgatgcccg tcgaggtcgt
    16861 cgtgcccttg ctcatggcgg cgccctcgcg gaacacggcc agcgccgcct cctccccctt
    16921 caggcggtcg accaccgggt gctccgcgtc gaccgtctcg tggttgttgg cgtacaacag
    16981 gaagtcgacg gggtaaccgc gcagcacgtc ctccaggctc gtgaccggga gcacgacccg
    17041 ggcgttgacc ttgttcgggc tcatgatgat ggagcggtcg agctggccga aggcatagcc
    17101 ctgctgcagg tcgtcgaggc gcacgaaggc gccgatctcg ctgccgaacc cgagcacccg
    17161 gccgtcgggg ccgacctcga gcgagcccat gtcgtcggcg atgatgcgca cctcgcggat
    17221 gcggtcctcg ccggccacgc gcagcgcctc gagcgtctcg ctcttgccgg tggccgtgtc
    17281 gccgatgagc agcaccgtcg cgcggctgcc gtcgtcgagg gtcaagcgcg cgtaggcgcc
    17341 gtggaacggc atcacgccgc gatccatggc ggcgacgttg tgcagcgtga gcaccatctt
    17401 cttgaggtac ccgaagtagc cgaagcggtc ctccagcggg accgccgcca cgaggagccg
    17461 gccgcggcgg tcgtcgtgga acaccgtcgg gaggtcgccg aagcgggcga gcgccgccgg
    17521 cgggacgccg tagagataca cggcgtcggg gccggcggcg atctcctcgt ccgaggcgac
    17581 ctcgaacagg ttggcgaggc tcagcccgag gcccatgaaa cggcggtgga agtagacgaa
    17641 caccgtcgtc cggcccacca gagccgggta gcagagccac tcctcccgct ccagcttgag
    17701 cccgtcgagc gggttctccg cggtctcggc gaactgcccc gtgcgcgtgt tcatcggcgg
    17761 gtcgagcagc agtggcgggt agaggagcag gtcgcggacg aacgggatgc cggtgagcag
    17821 ctcgcggtag cgagccggca ccggccactt gtggcgcacg gcgtacaccc cgacctcggc
    17881 tccggccgcg acctggcgca gcacgcgcgg ccgggtgccg gtgatgttct cggccacgtc
    17941 gcggtagagc gcccggacca gccgcgccag cgactcgacc gtctcggtga agccgcgcaa
    18001 cggtcgcttg tcgccgggcg ccggcgccac ctcggcgcgc accacgagaa agcggtccca
    18061 gcggcgccag aagtcgtaga ggccctcgac gaactcgtgc agctgctcgc gccgcgcgag
    18121 caggtcgccc ggagcgcagg cggtgatgga gacgcgctcc agcgggttgt tggcgagcag
    18181 ccggatcaga tcgaggagcc gcgcgcggcg agggtcgccg tccccgccgg ccggggcctc
    18241 gaggcccgcc cgcgcgggcc ctgagagccc gagctcgtcg agcgcctgcg ggttgtgcgc
    18301 ctccaggtgg tcgacgtaga gcccgagcac gcgggcgaag gtgacgctct ccacgagctc
    18361 ctgcgtgccg gcgcacacgg agccctccga gtacatgacc gcctggcggt cgtcgaggtc
    18421 cagtcgctgc agggacatgt gctctcctga ggtctcacgg gctcgccggc ggtgcgccgg
    18481 cgagcccgga gactaccaca ggaggccgct cctgtcggct cacagcaggc cgggggtgtc
    18541 cgccaggtgc ggtgggacgc atcgcagcat cgtggagcgt gcagccgcct gtgccgtcga
    18601 tctcctcgac gatatcaagg accggtctcg cgagcattcc tcccgctccg cctctcgcag
    18661 tccgtgcagc tgcaacgcgg ctcacgcagg tccgacgttc ccgccagctg ggcggtccgg
    18721 cgtcaggccg ctcgcgccgg cgtcagctcg cggccgccag ctcgctggtg cagtgcgggc
    18781 accgggtcgc gccgacgggg atctcgctca ggcagtgcgg gcactccctg gtcgtcggat
    18841 cctggccctt ggcgcgccgt tcggcgagct tgttcatcgg cagcacgacg acgaagtaga
    18901 cggcggcggc gatgagcagg aacgtgatca gcgcgttgag gaaggagccg tacttgatga
    18961 caccctcccc gatcttgaac gacaggctgc tgaagtcggg ctgcccgccg acggcggcga
    19021 tgatctgcat cagcacgtcg ttgacgaagg acgtgatcac cgccccgaac gcggcgccga
    19081 tgaccacggc gacagccagg tcgacgacgt tgccgcgcat gatgaagtcg cggaatccct
    19141 tgaacatgga gcccctcctc ctgtggcttc gcgccgcacc cggcgcactt caccccccga
    19201 gtctacccgc gggcgcgggg acgacaagga acgattggct cctcgatgga gcgggaaatg
    19261 acagaagacc gtccattgcg tggccggcgg ctccgaggta gactcccgca gccagcgacc
    19321 accacgactc acgaggaggc gaacagatga cagccaagac cgccagcggc acgaccgcat
    19381 cgtccctcgt gagggtcctg tcctgagcat gcgctcgctc tgagcggcgc tccggccggc
    19441 cgccctccag gcgccggccc ctcctgatcc ttccttcgcc ctgctgggcc cggtgcggga
    19501 cgacgcccgc gcgcgctggc gcgcacttcc cacacaccgt cccgaaagga cacaccagcg
    19561 atggatcacg tacacgtacg gcaacgactc caggccttcg ccgcccgcct ccggcgcgcg
    19621 gcaggtccgg cgggccacga gagctccgga gtggagcgcg ccgccgcgac ccgcgaggcc
    19681 tcggtcggcc tgcgcagcat gaacacgctc ggcgcaggcc ggcggtgaac ccgcacgccg
    19741 tccggcgccc ag
//
LOCUS       DASUMX010000162        10392 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_339586, whole genome shotgun sequence.
ACCESSION   DASUMX010000162 DASUMX010000000
VERSION     DASUMX010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 10392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10392
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339586"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /locus_tag="VFZ86_08140"
     CDS             <1..677
                     /locus_tag="VFZ86_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642293.1"
                     /translation="ADLGSVTLLRGTQAREAKRTLSLFNDLVILVVALTVALIVAALL
                     VSVRRLRTAIWLGLGTLLAFVVARLIAVRLEQAVVDAVETRGGAAVARSVLSSAVRSL
                     DAFFVWIAVAGAIVAVAALLAGRPTWLQAIGRGVAELFGVASDLSTPDTRAGRWMAAH
                     LDLLRILGVAVAVVVLLFVTGSPTAVIVVVLALVVYELALGAYAAGVPRELEEDAGGD
                     PPARAG"
     gene            complement(730..1563)
                     /locus_tag="VFZ86_08145"
     CDS             complement(730..1563)
                     /locus_tag="VFZ86_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642294.1"
                     /translation="MTLLLAAIPGNVLGDARRPLFALLVGFIVTFLIARLNTRLTRRH
                     GRSRMGIASVVTPGGLHIHHSVFGIIAMVIFGILTFALQPGSPWLEALAFFFGGAAAL
                     TLDEFALILHLEDVYWTGEGRKSIDAVILGVTFITLLLTGLLPRTFSEIGDYITLSRW
                     VLFVLILSGAIFVVVSYLKGKLFVGTVGIFVPPVALAGAFRLAKPGSPWARWRYRDRP
                     LALERARRRDEGFHQKWERRKHRLWDVIGGKPHLPLPHLDGHRDRHDRGPKDGSRETR
                     V"
     gene            complement(1680..2606)
                     /locus_tag="VFZ86_08150"
     CDS             complement(1680..2606)
                     /locus_tag="VFZ86_08150"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF459 domain-containing protein"
                     /protein_id="HEX5642295.1"
                     /translation="MQDGNNSAFTSRSSRDRRRRRRQARRRLLVLIIIIVVIVVAAAV
                     VVVLGRGGESEAAGGTGGAAAGATPSPRPTPATPPPKVWVASAGDPVRVWVGGDSMGG
                     ELGWALEPVLQDAGAFKPVTYYKVSSGICRYDFFDWQRQIEAVVKAKKPQAAVIMMGT
                     NDTQSVWMDGDWIPYGKMAWKQAYERRVGDIIDTLLEGGARRVYWVGMPIMGEDWRNS
                     RMRLINRIFRKQSEKRPGAEYVDIWDLYTTPDGSFDASLRLADQVHFTAEGQALLAGT
                     VYKAIKADWLPPGSEAPSASPAASPSASASAL"
     gene            2732..3559
                     /locus_tag="VFZ86_08155"
     CDS             2732..3559
                     /locus_tag="VFZ86_08155"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HEX5642296.1"
                     /translation="MDRPAVTPDAGPPARRRLLAESPRFADSFGLVLVLLVLSYFVIA
                     AGGDGDAGRIVSIVLFAATTWLALRAAQVKRRILRIALALIPLATLLAVVLMLAGSEE
                     TASIVGRVLTILLVVVAPIAILKRLVEHPVISLNTFYGAVCVYLLIAMFFATTYGLIA
                     LVSGEQFFAQLTVTPQDTPAVDYLYFSFVTITTTGYGDFSAATGFGRMTAVLEAIFGQ
                     LYLITVVALVVQNLGQQSRLGRRMEEEQRREAVAAEATEGVPDGVRTAGAPGPDEDA"
     gene            complement(3604..3996)
                     /locus_tag="VFZ86_08160"
     CDS             complement(3604..3996)
                     /locus_tag="VFZ86_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HEX5642297.1"
                     /translation="MSVRALAILMTGVIVFGVADVCYVLYKRLMAPPRGLLEITDILA
                     TFGAFMAVLIAIEIFVNIVSYLRDDVIHVRIVLATALMAIARKVIILDYSTTSSEYVY
                     ATAAVVAAMALAYWLVVRQRPADVTGAE"
     gene            4214..5551
                     /locus_tag="VFZ86_08165"
     CDS             4214..5551
                     /locus_tag="VFZ86_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015190710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEX5642298.1"
                     /translation="MNAYDSRLRRVVGGTFWLSLYVALSLTPLLVAFVYRDHPPRDFW
                     TEFSVALGFVALAMIGLQFAITARFRHIAAPYGIDIVLRFHRQISILAVAVAVAHPLI
                     LFATREDTRALLNVLTAPWRARFAVAGTLALLVLVAISVWRRRLRLRYEHWRTAHGVL
                     AILGLTFALVHIELVGWYVDLPWKRALWAAMSLAVICLLLYVRFVVPLRLLRLPWVVD
                     EVVGERGRAWTLVLRPVGHPGMKFLPGQFAWLRIGRSPFAIEDHPFSFSSSAEDGERI
                     AMTIKEAGDFTGGVGALTAGTPAYVDGPHGAFSIDRYEGKGCVFVAGGVGITPVMSML
                     RTLADRGDARPQLLVYGAGRWEDVTFREELEALRARLDLRIVYVLSEAPADWQGARGR
                     ITGELLAEHLPDDRAAYLYFVCGPPAMMDAAERSLLHAGVPWPSIYTERFDLV"
     gene            complement(5691..6209)
                     /locus_tag="VFZ86_08170"
     CDS             complement(5691..6209)
                     /locus_tag="VFZ86_08170"
                     /inference="COORDINATES: protein motif:HMM:NF015317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqhA family protein"
                     /protein_id="HEX5642299.1"
                     /translation="MVASSRFFVLLATLSLFLAFMAVMVSTIIVTVTTILHAIGGDST
                     QNEMIGKLIKQADYALLATVLYVLALGLYSLFVDDRVPMPLWLRIHDLGQLKELLAGV
                     VVVAIAVIFLGQALTWDGATDLLAPGLASAAVITALALFLWQAARERRSHEDGRRDDG
                     RHDDGEESRRRV"
     gene            complement(6265..6852)
                     /locus_tag="VFZ86_08175"
     CDS             complement(6265..6852)
                     /locus_tag="VFZ86_08175"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEX5642300.1"
                     /translation="MSAYGNGPDRPRPEVVSETVVVVDGRRAASGAWGLVLAVGIVAV
                     VFGILVLANIWGSVHLVAIFAGLFLLFAGVLQFFIGGGGKTGRIVAGVIAIVAGIALI
                     AWPEASVKTVAVIVGLAFLIWGASVAIAAISARGEGYGVVVGFGVLLAVVGLVFIIWP
                     GPTVTLLMILVGLSALLFGISAIVQALAMRRALHG"
     gene            complement(6899..7360)
                     /locus_tag="VFZ86_08180"
     CDS             complement(6899..7360)
                     /locus_tag="VFZ86_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HEX5642301.1"
                     /translation="MKAHTVYRTFRVPERRAFVRITDDVQRAVDESGIREGMALVAAM
                     HITAGVWINDDEPGLQADVLEWLDKLAPPSWQPPANDVARQLLPDDGDYRHHRGGEDN
                     GDAHLKNLLVHHQVIVPVTEGRLDLGPWQAVFYCEFDGRRAKRLIIKVLGE"
     gene            complement(7367..7891)
                     /locus_tag="VFZ86_08185"
     CDS             complement(7367..7891)
                     /locus_tag="VFZ86_08185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfcE family phosphodiesterase"
                     /protein_id="HEX5642302.1"
                     /translation="MVKSSGTARIGVISDTHGHLDPQVLEEFAGVDHIIHAGDIMDGA
                     TLEALTAIAPVTAVAGNMDDGKLGKLPREVAGKVGGVRFIVGHKRKRLLKRLALGKID
                     GVGKHESPDLVVCGHDHLPAVEWVDGTLFLNPGSASAPHYEDDDPTIAIVESGPVGLG
                     VRFVRVPRRADDEG"
     gene            complement(8015..8941)
                     /locus_tag="VFZ86_08190"
     CDS             complement(8015..8941)
                     /locus_tag="VFZ86_08190"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5642303.1"
                     /translation="MDDRIETTTLPADAGPRPPGTRMTFLGHASTVIEMDGVRLVTDP
                     VLRRTVGPLYRRVPRPLTGPLVDPDAILISHLHLDHYDPRSLRLFRRDTPVFAPVGAG
                     LSLRWRGFKDIHEVTPGERLRVGPLEVVATDARHRGTRHPLAARTPSLGYVVAGSHTV
                     YFAGDTAFFSGIADVWPERLDLALLPIAGIGPLMPEFKHMSPRHAVQAMELLRPRLVV
                     PIHWGTYHLPGTALFRFGPDFHRRAPFLFMAQAADLEPDVHTVMLRPGETLSLQETLA
                     GHEGHAVAGWPPRPQEPGPSELAGPGVPGGVL"
     gene            9097..9528
                     /locus_tag="VFZ86_08195"
     CDS             9097..9528
                     /locus_tag="VFZ86_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642304.1"
                     /translation="MATIQQQIEVAADPAHVRSTWAHFVEWAHTGPGHLLCDDLACVD
                     AVRSGLVDFVPAANGRTTVIFRLEEIRDGPGPAEVKRRLNHDLVVFKDYVERSGLVRR
                     KPTAAEEAAFEIEADRKGDPPRHVRLSSEGDTTFWRSHFPT"
     gene            9585..10358
                     /locus_tag="VFZ86_08200"
     CDS             9585..10358
                     /locus_tag="VFZ86_08200"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX5642305.1"
                     /translation="MRVASFNVRTGLGWDGCDSWPLRAGACAAAIAGLDADVVGLQEV
                     RLFQERGLSRRLPGYAGAGAGRDDGRGRGERCTVAYRPARLRLLSWTVRWFSDTPGRP
                     GSRSWGNPIARIVTLCRFGDGRTGAVFGVANAHWDGASPASRRRSAEALLGWLDPALP
                     WIVLGDLNATAGDPAVAHLLAAGLRDTLAHLGERGPQAGTHHPWDGSTDGTRIDYVLV
                     DRGWDVLAARIDHARPGGRLPSDHWPVVATVALLPAGAA"
BASE COUNT         1439 a         3698 c         3767 g         1488 t
ORIGIN      
        1 cggcggacct cggctccgtc acgctgctca gggggaccca ggcgcgcgag gcgaagcgga
       61 cgctcagcct gttcaacgac ctcgtgatcc tcgtggtagc gctcaccgta gcgctcatcg
      121 tggcggcgct gctcgtctcc gtgcgacgcc tgcggacggc gatctggctc ggcctcggga
      181 cgctgctggc cttcgtcgtg gcacgcctga tcgcggtgcg gctggagcag gccgtcgtgg
      241 acgcggtgga gacccggggt ggggcagcgg tggcgcgctc cgtcctgagc tccgcggtcc
      301 ggagcctcga cgcgttcttc gtctggatcg cggtcgccgg ggcgatcgtc gcggtggccg
      361 ccctcctcgc cggccggccc acgtggctgc aggccatcgg gagaggcgtc gccgagctct
      421 tcggtgtcgc ctccgacctc tccaccccgg acacgcgcgc cggccgctgg atggcggccc
      481 acctcgatct gctgcggatc ctcggcgtcg ccgtcgcggt cgtcgtgctg ctcttcgtga
      541 cgggctcgcc gaccgccgtg atcgtcgtcg tcctcgcgct ggtggtgtac gagttggcac
      601 tcggcgccta cgcggccggc gtgccccgcg agctcgagga ggacgccggc ggcgacccgc
      661 cggcccgggc cggctgacgc cacgcggccg gcggccgctc cggcgggctc cgtggactcc
      721 ggccgtcgcc tacacgcggg tctcgcgcga gccgtccttg ggaccgcggt cgtggcggtc
      781 gcggtggccg tcgagatgcg gcagggggag gtgcggcttg ccgccgatga cgtcccagag
      841 acggtgcttg cgccgctccc acttctggtg gaagccctcg tcgcggcgac gggcgcgctc
      901 cagcgccaac gggcggtcgc ggtaccgcca gcgcgcccac ggcgaacccg gcttggcgag
      961 gcggaacgcc ccggccagcg cgaccggcgg tacgaagatc cccaccgtgc cgacgaacag
     1021 cttgcccttg aggtagctca cgacgacgaa gatggcgccg ctcaggatga gcacgaacag
     1081 cacccagcgc gagagggtga tgtagtcgcc gatctcgctg aaggtgcgcg gcaacagccc
     1141 cgtgagcagc agcgtgatga aggtgacgcc caggatgacg gcgtcgatgc tcttgcggcc
     1201 ctcgccggtc cagtacacgt cctcgaggtg caggatcagg gcgaactcgt cgagggtgag
     1261 ggccgccgcg ccgccgaaga agaaggcgag cgcctcgagc cacgggctcc ccggctgcag
     1321 ggcgaacgtg aggatcccga agatcaccat ggcgatgatg ccgaacaccg agtggtggat
     1381 gtgcaggccg cccggcgtga cgacgctggc gatgcccatg cgcgaacggc cgtggcgccg
     1441 ggtcagacgg gtgttgagcc gggcgatgag gaacgtgacg atgaagccga cgagcagcgc
     1501 gaacagcggg cgcctggcgt cgccgagcac gttaccgggg atggcggcga ggagaagggt
     1561 catgacactc ccattatcct cccgcggcgg cgagcggaat catcgccgcc cgccggggcg
     1621 cgaggggagc gtgcagcgtg ccggcgcgga cgcggcggag gacgccggcg cgcggccggt
     1681 cagagcgcgg aagcggaggc cgaggggctc gccgccggcg aggcgctcgg cgcctccgac
     1741 cccggcggca gccagtcggc cttgatggcc ttgtagaccg tcccggccag cagtgcctgc
     1801 ccctccgcgg tgaagtgcac ctggtcggcg agccgcagcg acgcgtcgaa cgagccgtcg
     1861 ggggtggtgt agaggtccca gatgtcgacg tactcggccc cgggccgctt ctccgactgc
     1921 ttccggaaga tcctgttgat cagccgcatg cgtgagttgc gccagtcctc ccccatgatc
     1981 ggcatgccga cccagtagac gcgccgcgcg ccgccctcga gcagggtgtc gatgatgtcg
     2041 ccgacgcgcc gctcgtaggc ctgcttccag gccatcttgc cgtaggggat ccagtcgccg
     2101 tccatccaga ccgactgggt gtcgttggtc cccatcatga tcacggcggc ctgcggcttc
     2161 ttcgccttca ccaccgcctc gatctgccgc tgccagtcga agaagtcgta gcggcagatg
     2221 ccgctcgata ccttgtagta ggtgacgggc ttgaacgcgc cggcgtcctg caggacgggc
     2281 tccagggccc agccgagctc gccgcccatc gagtcgccgc cgacccacac gcggacgggg
     2341 tcgccggcgc tcgcgaccca gaccttgggc ggcggggtgg ccggcgtggg gcgcggtgag
     2401 ggcgtggcgc ccgccgccgc gccgcccgtg cctccggcgg cctcgctctc gccgccgcgc
     2461 ccaagaacga cgaccacggc ggcggcgacc acgatgacga cgatgatgat gatgagcacc
     2521 aggagacgac gccgtgcctg ccgcctgcgc cggcgccggt cgcggctgga acgcgacgtg
     2581 aacgcggagt tgttgccgtc ctgcatgggc tcggggacct ctcggcgagt gtacaggaag
     2641 gggttcgcgc cgcgctcagc ggcacctgcc gggcgcgtga gcccccggcc gtttcgcacg
     2701 accacgcggt gggatacagt gtcgcctgac gatggaccga ccggccgtga cacccgacgc
     2761 gggacccccc gccaggcgac gtctcctggc ggagtcgccg cggttcgccg acagcttcgg
     2821 gctcgtcctc gtgctgctcg tcctcagcta cttcgtgatc gcggcgggag gcgacggcga
     2881 cgccggtcgc atcgtctcca tcgtcctctt cgcggccacc acctggctcg cgctgcgcgc
     2941 ggcgcaggtc aagcgccgca tcctgcgcat cgcccttgcc ctcattcccc tggccaccct
     3001 gctggcggtc gtcctcatgc tcgccggcag cgaggagacc gccagcatcg tcggcagggt
     3061 cctgacgatc ctgctggtgg tcgtggcccc gatcgcgatc ctgaagcgcc tcgtggagca
     3121 ccccgtgatc agcctcaaca cgttctacgg cgccgtgtgc gtctacctgc tcatcgcgat
     3181 gttcttcgcc accacctacg gcctcatcgc cctggtcagc ggggagcagt tcttcgccca
     3241 gctcaccgtg accccccagg acacgccggc cgtcgactac ctctacttca gcttcgtgac
     3301 catcaccacg accgggtacg gggacttcag cgccgcgacg ggcttcgggc gcatgacggc
     3361 ggtgctcgag gcgatcttcg gacagctcta cctcatcacg gtcgtcgcgc tcgtcgtcca
     3421 gaacctcggg cagcagagcc ggctcgggcg gaggatggag gaggagcaac gcagggaggc
     3481 cgtcgcggcg gaagcgacgg agggcgtgcc ggacggcgtc cggacggccg gcgcaccggg
     3541 gccggacgag gacgcctgag gagtcccggc gccgcggccg gcctcacccg cccgcgccct
     3601 ccctcactcg gctccggtga cgtcggccgg ccgctggcgc acgaccagcc agtaggcgag
     3661 cgccatggcg gcgaccaccg ccgcggtcgc gtagacgtac tccgaggacg tcgtggagta
     3721 gtcgaggatg atgaccttgc gggcgatggc catgagcgcc gtggcgagga cgatgcgcac
     3781 gtggatgacg tcgtcgcgca ggtagctcac gatgttgacg aagatctcga tggcgatcag
     3841 gacggccatg aacgcgccga acgtggccag gatgtcggtg atctcgagca ggccccgggg
     3901 cggggccatc agccgcttgt acagcacgta gcagacgtcg gcgacgccga acacgatgac
     3961 gccggtcatc agaatggcca gcgcccgtac cgacatgcgg atgacccagt gcagccccct
     4021 gacgaggggc tcgtcgtcgg gtcccagttg ctccgccaca cggacctccg gatcttccgc
     4081 ggcgatgcgc gcgcaccgcc gcccgtctga gccggtttcg gcgcgccgcc gacgttccct
     4141 tcgcggcgcc gtgacgccgc gacggcggag cgcggcgcgt gaagagcggg cggcccatgc
     4201 gtacactggt cacatgaacg cgtatgactc gcggctgagg cgcgtcgtcg gcggcacgtt
     4261 ctggctgtcg ctctacgtgg ccctgtcgct caccccgctc ctcgtggcct tcgtctaccg
     4321 ggaccatcca ccccgggact tctggacgga gttctccgtc gccctcgggt tcgtcgcgct
     4381 ggcgatgatc ggcctccagt tcgccatcac cgcccggttc cgtcacatcg ccgcgcccta
     4441 cggcatcgac atcgtcctcc gcttccaccg gcagatctcg atcctcgcgg tcgccgtggc
     4501 ggtcgcgcac ccgctgatcc tcttcgccac tcgagaggac acgcgggcgc tgctcaacgt
     4561 cctgacggcg ccgtggcgag cccgcttcgc agtcgccggg acgctggcgc ttctcgtcct
     4621 cgtggcgatc tccgtctggc gccggcggct gcggctgcgc tacgagcact ggcggaccgc
     4681 ccacggagtc ctcgccatcc tcggcctcac cttcgccctg gtccacatcg agctcgtcgg
     4741 ctggtacgtg gaccttccct ggaagcgtgc gttgtgggcg gccatgagcc tggcggtcat
     4801 ctgtctgctc ctctacgtgc gcttcgtggt ccccctccgg ctgctccgcc tgccgtgggt
     4861 cgtggacgag gtcgtgggcg agcgcggccg ggcgtggacg ctcgtgctgc ggccggtggg
     4921 ccaccccggc atgaagttcc tgccgggcca gttcgcctgg ctcaggatcg gacgatcgcc
     4981 gttcgccatc gaggatcacc cgttctcgtt ctcctccagc gcggaggacg gtgagcggat
     5041 cgcgatgacc atcaaggagg ccggcgactt cacgggaggc gtcggcgcgc tgacggcggg
     5101 cacgccggcc tacgtcgacg gcccgcacgg cgccttcagc atcgaccggt acgagggcaa
     5161 gggctgcgtg ttcgtcgccg gcggcgtggg catcaccccc gtgatgagca tgctccgcac
     5221 cctggccgat cgcggcgacg cgaggccgca actcctggtg tacggcgccg gccggtggga
     5281 ggacgtgacg ttccgcgagg agctcgaagc gcttcgcgcc cgcctcgacc tgcgcatcgt
     5341 ctacgtgctg tccgaggcgc cggccgactg gcagggagcc agagggcgga tcaccgggga
     5401 gctcctcgcc gagcacctgc cggacgacag agcggcgtac ctctacttcg tctgcgggcc
     5461 cccggcgatg atggacgcgg cggagcggtc gctcctgcac gccggggtcc cttggccgag
     5521 catctacacg gagcgcttcg atctggtatg aagcgccgca tggactggag gcggtgaacc
     5581 tgcgtcacgt gcacaccacc cgggtcgtcg ctgtcgtggc cgcgctcgtc gtggccgcgt
     5641 cggtggcctt cgggcttctg cgctcctgac gagcggccgc gctgctcgcg tcagacgcgg
     5701 cgcctgctct cctcgccgtc atcgtgccgg ccgtcatcgc gccggccgtc ttcgtgcgag
     5761 cgccgctcgc gcgcggcctg ccacaggaag agcgccagcg cggtgatgac ggcggccgag
     5821 gcgaggcccg gcgccagcag atcggtcgcg ccgtcccagg tcagcgcctg ccccaggaag
     5881 atgacggcga tcgccaccac gaccacgccg gcgagcagtt ccttgagctg accgagatcg
     5941 tggatgcgca gccagagcgg catcggcacg cggtcgtcga cgaagaggct gtagagaccg
     6001 agcgcgagca cgtagagcac cgtggcgagc agcgcgtagt cggcctgctt gatcagcttg
     6061 ccgatcatct cgttctgcgt gctgtcgccg ccgatggcgt gcaggatcgt ggtgacggtg
     6121 acgatgatcg tcgacaccat gaccgccatg aacgcgagga acaggctcag ggtcgccagg
     6181 aggacgaaga agcggctgga cgcgaccatg cgcccgatca tgtcggacgg cgggcggcgc
     6241 ccatcttccg gcgcggcgct cacgtcaccc gtgcagcgca cgccgcatgg cgagcgcctg
     6301 gacgatcgcc gagatgccga agagcagcgc ggacaggccg acgaggatca tcagcaacgt
     6361 caccgtcgga cccggccaga tgatgaaaac gagcccgacg acggcgagca ggacgccgaa
     6421 gccgacgacg acgccgtagc cttctccgcg cgcgctgatg gcggcgatcg ccacagacgc
     6481 gccccagatg aggaacgcga gcccgacgat gacggcgacg gtcttgaccg acgcctcggg
     6541 ccacgcgatg agcgcgatgc cggcgacgat ggcgatgaca ccggcgacga tgcggccggt
     6601 cttgccgccg ccgccgatga agaactgcag gacgcccgcg aagaggagga agaggccggc
     6661 gaagatggcc accaggtgca cgctgcccca gatgttggcc aggaccagga tgccgaagac
     6721 gaccgcgacg atgccgacgg cgagcacgag gccccaggcc ccgctcgcgg cccggcggcc
     6781 gtcgacgacg accaccgtct cgctcaccac ttccggacgc ggccggtcag gtccgttccc
     6841 gtacgcgctc atgtgcacct ccgctctcgt ctggccgcca ctgccctggg cggggagtct
     6901 attccccgag gaccttgatg atgagccgct tggcgcgacg gccgtcgaac tcgcagtaga
     6961 agacggcctg ccaggggccc aggtcgaggc gcccttccgt gaccggcacg atcacctggt
     7021 ggtggacgag caggttcttg agatgggcgt cgccgttgtc ctcgccgccg cgatggtggc
     7081 ggtagtctcc atcatcgggg agcagctgcc gggccacgtc gttggccggc ggctgccagg
     7141 acggcggcgc cagcttgtcg agccactcga gcacgtccgc ctggagaccc ggctcatcgt
     7201 cgttgatcca cacgccggcg gtgatgtgca tggccgcgac cagcgccatc ccttcgcgga
     7261 tgccgctctc gtccacggct cgctgcacgt cgtcggtgat gcgcacgaac gcgcgccgct
     7321 cgggcacgcg gaaggtcctg tagacggtgt gcgccttcac gggtcctcag ccctcgtcgt
     7381 cggcccgccg cggtacgcgc acgaagcgca cgccgagccc caccgggccg gactcgacga
     7441 tcgcgatggt cgggtcgtcg tcctcgtagt gaggggcgct ggccgacccc gggttgagga
     7501 agagcgtgcc gtccacccac tccaccgccg gcaggtggtc gtgcccgcac accacgaggt
     7561 caggggactc gtgcttgccg accccgtcga tcttgccgag cgccagccgc ttgagcagcc
     7621 gcttgcgctt gtggccgacg atgaagcgga cgccgccgac cttgccggcg acctcgcgcg
     7681 gcagcttgcc cagcttgccg tcgtccatgt tgccggccac ggcggtgacc ggcgcgatcg
     7741 ccgtcaatgc ctcgagggtg gcgccgtcca tgatgtcgcc ggcgtggatg atgtggtcga
     7801 cgccggcgaa ctcctcgagg acctgggggt cgagatggcc gtgcgtgtcc gagatgacgc
     7861 cgatgcgtgc agtcccactc gacttgacca tcaccctcct ccgggtcgca ggaggtcatc
     7921 atgccacccc ggcgcccccc aacgccatac tccccggcga gaggctgcgc ggaacccggc
     7981 gcgagggcga cgggtcgagg ccgcccgcgc cgctctagag gacgccgccg gggacgccgg
     8041 ggccggcgag ctcggagggc ccgggttcct gggggcgcgg cggccagccg gccacggcgt
     8101 ggccctcgtg gccggccagc gtctcctgca gggagagggt ctcgcccggg cgcagcatca
     8161 cggtgtgcac gtccggctcg aggtccgccg cctgcgccat gaagaggaac ggcgcccgcc
     8221 ggtggaagtc cgggccgaag cggaacaggg cggtccccgg caggtggtag gtgccccagt
     8281 ggatgggcac gacgaggcgc ggccgcagca gttccatggc ctggaccgcg tgccgcgggc
     8341 tcatgtgctt gaactcgggc atgagcggcc cgatgccggc gatgggcagc agcgcgaggt
     8401 cgagccgctc cggccacaca tcggcgatgc cggagaagaa cgccgtgtcg ccggcgaagt
     8461 acacggtgtg ggaccccgcg acgacgtagc ccaggctcgg ggtccgcgcc gccagcgggt
     8521 gccgggtccc gcggtgcctg gcgtccgtgg cgacgacctc cagcgggccg acgcgcagcc
     8581 gctcgccggg cgtcacctca tggatgtcct tgaacccgcg ccagcgcaga gacaggcccg
     8641 cgcccacggg cgcgaagacg ggggtgtcgc ggcggaacag gcgcagcgag cgcgggtcgt
     8701 agtgatcgag gtggaggtgc gagatgagga tcgcgtccgg gtcgacgagc gggccggtga
     8761 gcggccgcgg cacgcgccgg tagagcggcc ctaccgtccg tcgcagcacc gggtcggtga
     8821 cgaggcggac gccgtccatc tcgatgacgg tggaggcgtg cccgaggaac gtcatccggg
     8881 tccccggcgg ccgtggcccg gcatcggccg gcagcgtcgt cgtctcgatc cgatcgtcca
     8941 tgctcctcag tgtacggtgt ggccgcgccg ggtgcgcctc aaaccagcga gccgccgcgc
     9001 ccatctcatt cctgacgcga atcgtgagat ttcaccgtct ccacagcggg tagcctcgaa
     9061 caagcttgcc gggatcgatg gaagaacgga gtcaccatgg cgaccatcca gcaacagatc
     9121 gaggtggcgg cggaccctgc ccacgtgcgc tccacctggg cccacttcgt ggaatgggcg
     9181 cacaccggac ccggccatct cctctgcgac gatctcgcct gtgtggacgc ggtgcgttcc
     9241 gggctcgtcg acttcgtgcc ggcggcgaac ggccggacga cggtgatctt ccgccttgag
     9301 gagatccggg acggtcccgg ccccgcggag gtcaagcgcc ggctcaacca cgacctcgtc
     9361 gtcttcaagg actacgtcga gcggagcggc ctggtgcggc gcaagccgac cgccgcggaa
     9421 gaggcggcgt tcgagatcga ggcggaccgc aagggcgacc cgccgcggca cgtgcggctg
     9481 agcagcgagg gagacaccac cttctggcgg agccacttcc ccacgtgagc gccggcgccg
     9541 agggcgacga gcaacgaggc cggcgatcgg gtccaccggc ctcgttgcgc gtcgcttcct
     9601 tcaacgtgcg caccggcctg gggtgggacg ggtgcgacag ctggccgctg cgggccggcg
     9661 cctgtgcggc ggcgatcgcc ggcctcgacg ccgacgtcgt cggcctgcag gaggtccgcc
     9721 tcttccagga gcgggggctg tcgcgccggc tgcccggcta cgccggcgcc ggcgccggtc
     9781 gtgacgacgg ccgcgggcgc ggcgaacgct gcacggtcgc gtatcgcccg gcgcggctgc
     9841 ggttgctgag ctggacggtc cgctggttct ccgacacgcc cgggaggccc gggtcgcgct
     9901 cctggggcaa cccgatcgcc cgcatcgtca ccctctgccg gttcggcgac gggcggacgg
     9961 gggcggtctt cggcgtcgcc aacgcgcact gggacggcgc ctcgcccgcc tcgcggcgac
    10021 gcagcgccga ggcgctcctc gggtggctgg acccggcgct cccctggatc gtcctgggag
    10081 acctgaacgc gacggcagga gacccggccg tcgcgcacct gctcgccgcc ggcctgcgcg
    10141 acacgctcgc ccatctgggc gagcgcgggc cgcaggccgg cacgcaccac ccgtgggacg
    10201 ggtcgaccga cgggacgcgc atcgactacg tgctcgtcga ccgcggctgg gacgtcctgg
    10261 ccgcgcgcat cgaccacgcc cggccggggg gacgtctgcc ctcggaccac tggccggtgg
    10321 tggccacggt cgcgctgctg ccggccggcg cggcctgacc ggcccccgcc acggggacgc
    10381 cgccggcgac cc
//
LOCUS       DASUMX010000163         2275 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1923560, whole genome shotgun sequence.
ACCESSION   DASUMX010000163 DASUMX010000000
VERSION     DASUMX010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1923560"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="VFZ86_08205"
     CDS             <1..496
                     /locus_tag="VFZ86_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642306.1"
                     /translation="LYLIGAVTRRQGEVVVLRVKPPTFPKGASEYGETQVRYWSLNET
                     NRNTSTPLGLRDDQFKVASDGFVYVAIGDKGIRRQARQRGYNFMPWRIRGQTGAVIYR
                     NMVTDPSYAGSIDKVPILDLSDSRNIYAQNATRFIGDYAPSGAVISLRRFLKGGGRIT
                     PPRR"
     gene            complement(509..2086)
                     /locus_tag="VFZ86_08210"
     CDS             complement(509..2086)
                     /locus_tag="VFZ86_08210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642307.1"
                     /translation="MVPVLTTFAALAALLAGPAMASPNWPGWAPPALAAQGATSYPEV
                     PPGPATVLPDFVGSAVAPHPLPNARVPQNPYLGQNPYGYLHDDSWNSDTADGPGPLGH
                     DLETISSTLGLPAVLQWASSTSSLSFDSHGRLVVAFLQDGLVTGASVLLLDPVSLDVL
                     FSYPFGTSAIGSIYFYVDDQDRVVMATGPRAITTLRERGTEAAPTLELVPERSYDLSR
                     VIPEGDHLAGLLPDWDGNILFATAGEGKATGPRVGVVDPDARPRVKWVELGKGEYMAN
                     GMAVTRTASYAITSKALYKLTVGDGRPRVEWRAGYDHSVDKSQLGQLCVGSGTSPTIL
                     GGGKYVTINDSATPMKVLVFRTATKLQPGQRRLIASIPVFADMAGQAAANSILGYRDS
                     MVIQNSYGHSDAYDKDGFLRSTDSLPGLERIDIKPGGKGLRKVWVNDEVTSVNLPKLS
                     TQTGLIYVIDRQDDENGVDAYYWTAVDFRTGKVVWRKLAGTGVAWDSYFAPSSLGPTG
                     TFYNGNYGGLAAVRDGK"
BASE COUNT          359 a          752 c          801 g          363 t
ORIGIN      
        1 cctgtacctc atcggcgccg tcacgcggcg gcagggcgag gtcgtggtgc tgcgcgtcaa
       61 gccgccgacg ttcccgaaag gcgcgtccga gtacggcgaa acgcaggtgc gctactggtc
      121 gctcaacgag acgaaccgga acacgtccac gccgctcggc ttgagagacg accagttcaa
      181 ggtggcctcc gacggcttcg tctacgtggc gatcggcgac aagggcatcc gcagacaggc
      241 caggcagcgc ggctacaact tcatgccctg gcgcatcaga gggcagaccg gcgccgtgat
      301 ctaccggaac atggtcacgg acccgtccta cgccggcagt atcgacaagg tgccgatcct
      361 cgacctcagc gactcgcgga acatctacgc gcagaacgcc acgcgcttca tcggtgacta
      421 cgcgccgtcg ggtgcggtca tcagcctgcg gcggttcctc aagggtgggg ggaggatcac
      481 accgccgcgg cggtagaaca ggggcgggtc acttcccgtc ccgcacagcg gcaagccctc
      541 cgtagttgcc gttgtagaac gtccccgtgg gcccgaggga cgacggcgcg aagtagctgt
      601 cccaggccac accggtgcct gccagcttgc gccacaccac cttgccggtg cggaagtcca
      661 cggccgtcca gtagtacgcg tcgacgccgt tctcgtcgtc ctgcctgtcg atgacgtaga
      721 tgagcccggt ctgcgtggac agcttgggga ggttgacgga ggtgacctcg tcgttcaccc
      781 agaccttgcg cagccccttg ccgcccggct tgatgtcgat gcgctccagg ccgggcaggc
      841 tgtccgtcga ccggaggaag ccgtccttgt cgtaggcgtc cgagtgcccg tagctgttct
      901 ggatgaccat ggagtcgcgg tacccgagga tagagttagc ggcggcttgg cccgccatgt
      961 ccgcgaacac cggtatggat gcgatgagcc tgcgctgtcc cggctgcagc ttggtcgccg
     1021 tacggaagac gaggaccttc atcggcgtgg cgctgtcgtt gatcgtcacg tacttgccgc
     1081 ccccgaggat ggttgggctg gtccccgaac cgacgcacag ctgccccagc tgggacttgt
     1141 cgacgctgtg gtcgtacccg gcacgccact ccacacgtgg ccggccgtct ccgacggtga
     1201 gcttgtacag cgccttggat gtgatggcgt acgaggctgt cctggtcacg gccatcccgt
     1261 tcgccatgta ctcgcccttg ccgagctcca cccacttcac ccgcggccgc gcgtccgggt
     1321 cgacgacgcc cacgcgcggc cctgtcgcct tcccttctcc ggccgttgcg aagaggatgt
     1381 tgccgtccca gtcgggcagg agccccgcga gatggtcgcc ctccgggatc acccgcgaga
     1441 ggtcgtagct tcgctcgggc acgagctcga gcgtgggcgc ggcctctgtc ccgcgctcgc
     1501 ggagcgtggt gatggcgcga ggaccggtgg ccatcacgac gcggtcctgg tcgtcgacgt
     1561 agaagtagat gctcccgatc gcgctggtgc cgaaggggta gctgaacagg acgtcgagtg
     1621 acacggggtc gagcaggagg acggacgcgc cggtcacgag gccgtcctgc aggaaggcga
     1681 cgacaagacg tccgtgactg tcgaaggaga ggctgctggt gctcgacgcc cactgcagaa
     1741 cggcaggcaa gccgagcgtg gaggagatgg tctcgaggtc gtggccgaga ggtccgggcc
     1801 cgtcggcggt gtcgctgttc cagctgtcgt cgtgcaagta gccgtagggg ttctggccga
     1861 gatacgggtt ctgaggaacg cgagcgttcg gtagcgggtg tggagcgacc gcggagccga
     1921 cgaagtcggg cagcacggtc gccggcccgg gaggcacctc ggggtaggag gtggccccct
     1981 gggctgccag ggcgggcgga gcccagccgg gccagttcgg tgacgccatg gcgggtccgg
     2041 cgagcaacgc agccagcgcc gcgaaggtcg tcagtacggg aaccatcgtc cacactcgcc
     2101 tcatgacccc ctccagtcac gtcgcggtcg aacggccggg cgtccccacg cgccatcagc
     2161 ctcgccttga agcatgtcac gattcggcgc ggtgggccag agacgggggc cggcgcggca
     2221 gccgccttgc ggtcaccgcg ccggctccca ctccccgaga ctcagccggt gagcg
//
LOCUS       DASUMX010000164        10000 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_54391, whole genome shotgun sequence.
ACCESSION   DASUMX010000164 DASUMX010000000
VERSION     DASUMX010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 10000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10000
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54391"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(219..1085)
                     /locus_tag="VFZ86_08215"
     CDS             complement(219..1085)
                     /locus_tag="VFZ86_08215"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit B family protein"
                     /protein_id="HEX5642308.1"
                     /translation="MTKTLAYYPGCSLHGTSGEFDASFRATARALDLVLQEIPGWECC
                     GNTAAHSINRLLSVALPVNELAKVEQDMKLDAVAVPCAACYSRFKTGAHEVAESEETA
                     ADVRAVVGRAYNGGVEVDNLIDVYHDAVGLEELAAKVTRPFAGLKVACYYGCLLTRPP
                     KVTLADDPEYPTHMDEVCEAVGCEAVDWNYKTDCCGASLALTEQGIVDDLVGRILTDA
                     KACGAQAVIVACPLCQVNLDGRQVEIAKKDAGWQGIPVVFLSQLVGRALGLDDKTLGL
                     KKHLVDITAVMA"
     gene            complement(1082..1666)
                     /locus_tag="VFZ86_08220"
     CDS             complement(1082..1666)
                     /locus_tag="VFZ86_08220"
                     /inference="COORDINATES: protein motif:HMM:NF024581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX5642309.1"
                     /translation="MTATCTPSASLSRQVTAGCDENAYKCYQCKKCTSGCPVARYSDM
                     HPAMIMRAVQLGQFDMIFDDRFIWLCTGCETCTTRCPQSIDIATIIDELKIMARKQGR
                     IPADTPSAQMLKLNYDSFVRWGRMWEVELITLDVLKRPTSMKNWFTLGPRMLLKGKIN
                     PTLKRGDTMAMKRMVQTAESITRAREARNAGGRS"
     gene            complement(1676..2620)
                     /locus_tag="VFZ86_08225"
     CDS             complement(1676..2620)
                     /locus_tag="VFZ86_08225"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006968224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEX5642310.1"
                     /translation="MTATEYKAGTNLEKIFSEGKFAVTGECGPPKSADGGVIAGKADM
                     LKGYVDAVNITDNQTAVVRIASLPSAVTVQQHGLEAIMQMTCRDRNRLAMQADLLGAH
                     TLGVHNVLCLTGDHMSFGNHPEAKGVHDLDAINLIQMLKDMRDEKKFQCGDEMDVAPK
                     FYIGCAENPFGDPFEFRSIRLKKKILAGADFMQTQCIFNVPKFKKWMEEVRAEGLHQE
                     IKIMAGLTPLKGVGGARYMKNFVPGMDVPDDIVERMAGVEKGPASHAEGKKLAVEMIK
                     ELAEVEGVAGVHIMAVEWEEAVPEIVEAAGLMPRPTVA"
     gene            complement(2617..3300)
                     /locus_tag="VFZ86_08230"
     CDS             complement(2617..3300)
                     /locus_tag="VFZ86_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014827467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HEX5642311.1"
                     /translation="MITAERKPLDEILALVGDKKKVLVAGCDTCVAICLTGGEKEAEI
                     LASELRIKAEQDGREAEIEHTAAIRQCEWEYLDMIADKVKDADIVVSMACGIGIQSMA
                     EKFSPQLVVPAVNTNMLGMPQEHAVWLERCGACGDCVIGETAGICPVVRCSKSLMNGP
                     CGGSHDGLCEVKTPEKEDLDCAWHLIWERLKEQGREDLMIKNRPPKKWNLSSSGGPRK
                     MVREEAKPV"
     gene            complement(3405..3791)
                     /locus_tag="VFZ86_08235"
                     /pseudo
     CDS             complement(3405..3791)
                     /locus_tag="VFZ86_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822575.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
     gene            complement(3901..8346)
                     /locus_tag="VFZ86_08240"
     CDS             complement(3901..8346)
                     /locus_tag="VFZ86_08240"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5642312.1"
                     /translation="MSGSPPKSTGAALVVGGGVAGIQCSLDLAEGGYKVYLVEKAPAL
                     GGHMAQLDKTFPTNDCSMCTLAPKLVEAGRHLNIEIVTNSELIGLEGEPGSFDAKIYH
                     HARFVDPDICTSCGECSPVCPVTVPDPYNEGLNDRKAITKLYAQAIPNTYFVTKKGHS
                     PCKRACAVHTSAQGYLALIADGRYAEAYTVASDPNPFTAVCGRVCTHKCETDCTRGDV
                     DEPLAIAGLKRFVSDYAFANVPLPEKAEVTYKEKVAVVGAGPSGLTAARDLALLGYAV
                     TVFESKPEPGGMLRFGIPEYRLPKAALQQDIDRVLALGVDLQCDKAAGKDFTVDGLMK
                     KDGGGYKAVYLAVGLQGGRTLPVPGADAKGVLDAVSVLRAASLGEKVDMGKNVVVIGG
                     GDVAFDAGRTALRLGAEKVTINCIEDDQSVPASDDELTEGLDEGISFNCSCMPSQIVT
                     DASGRASKVEFLACSLGDPDERGWRPPVAIAGSEHELECDTVIFAIGQSMVDDFLEGS
                     KGITVEKGQILTDKATLGTGKKGVFAGGDAAASAPWTAIEAVAAGRRAARSIHNFLRG
                     EDLLPIEDDVMDEAKPEDAVLEQTAVMARLPMPHLPGAARKIVWDEVNTGFSEEQAVA
                     EAKRCLDCAICSECMECVRACGPGALLHGERDEEIDVEVGAVVMATGFDLHDPGDKGE
                     YGYQRYPNVLSALEYERMLSASGPTIGSVQRRSDGAHPKKIAFIQCVGSRDQEHEYCS
                     SVCCMFANKQAMLTIDHEPDCEPTVFLMDMRAQGKGFDAFYQRALDKGVGFIRSRPSY
                     IKEDPLTRDLLISWEDEGGRLHETRYDMVVLSAGLEPARKAQEAAGHLGVALNRHGFC
                     ELHEFNPLETSRKGVFVVGPFGEPKDIPDSVAQASAAAAQVMTGLADSRHTLTVDKEY
                     PAEKNVAGVEPRVGVFVCHCGSNIAGVIGVESVAEYAKELPGVAFATDTMYTCSSDSL
                     SLIRDAIEEHDLNRVVVASCTPRTHEPIFQDTLREAGLNPFLFEMANIRDQASWVHAN
                     EPEKATQKAKELVQMSVARARLLEPLFKVDVPLTHAAVVVGGGVAGMTSALALGDMGH
                     EVHLIERSPKLGGHVLELGDTIKGNDPTALVTQLEQRLVDNPNVKIHLEAELADFHGF
                     IGNFSSIIGEKDGKRTPVDHGVVVVATGGVEERPELYGLGSGQKVVTGMDLERMLKEH
                     DPALAAVKSVGFILCAGSLDENKPYCSRTCCAQSIKNAIRLKEQDPDRQVYVWFKEVR
                     TFGLLEEYYTRARELGVIFTRYDNDSKPEVSANGSVSVKARDPYLRRDVELPLDLLVL
                     ATPTVPSTGGSELSKLLKVPLTADGFYLEAHVKLRPVDFGSEGIFLCGAAHYPKSIEE
                     TISQAYAAAGRAASILAKSVLKAGGVVAEVDTEKCAACLTCVRICPYEVPIIDLETKK
                     AKIEAAACQGCGVCVSECPVKAITLHHYTDAQVFAKEEALFMEVS"
     gene            8586..9869
                     /locus_tag="VFZ86_08245"
     CDS             8586..9869
                     /locus_tag="VFZ86_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX5642313.1"
                     /translation="MGVQEQYRDYVMTGFVKRVEPVVADHGDGAYLYDADGTEYIDCF
                     AGISVTNAGHHNQQILDAAIAQANKLVHACSYVYHVEPVGALAQKLAEVTPGTLQKTF
                     FGNGGAEAIEGAFRLAKRYSGKREFLAIESSFHGRSFATLSVTGNSGRKKGGGPYMPG
                     VAFTPTPYCYRCFAETTPDRCDLLCARRVGEIARLHLSGDVCAFIAEPVMGEGGIIVP
                     PDGYFKRVKEILDEMGILFIADEVQSGFGRTGKLFAIDHYGVEPDIMTLAKGIADGWP
                     LSAFIAKPEIADAFQIGDHLSTFGGNPVSCAAALANIDFLLDGVVEGAARKGEALMAK
                     FRELAKTQPLIGDVRGKGLMVGVELIADPATKAYGTAQAGFVRQYCLDHGVLIGVGGN
                     FGSVLRIQPPLVIDDAQLDRVFATVADALEAAPRQ"
BASE COUNT         1400 a         3327 c         3543 g         1730 t
ORIGIN      
        1 cgacctgcgc agccctgtgc cgtgccttcc acgcctacac ccgctcaggc gtccgcgtca
       61 acgttggcgg cgcctgttgt caggagaggc aacgcaccgt tccggtgccc tcaggtgggt
      121 ccgtacgtga agaggggcgc cgcgaccgcg gcgcccctct tcgtatctcg gcgtggaccc
      181 tcgtgccggc gcagccggcc tgcaggcggc gcgacgcgct acgccatcac cgccgtgatg
      241 tccacgaggt gcttcttgag gccgagcgtc ttgtcgtcca gaccgagggc acggccgacg
      301 agctggctga ggaacaccac ggggatgccc tgccagccgg cgtccttctt ggcgatctcg
      361 acctggcggc cgtcgaggtt cacctggcag agcgggcagg cgacgatcac ggcctgcgcg
      421 ccgcacgcct tggcgtcggt gaggatgcgg ccgacgaggt cgtcgacgat gccctgctcg
      481 gtgagcgcca gtgaggcgcc gcagcagtcg gtcttgtagt tccagtccac ggcctcgcag
      541 ccgacggcct cgcagacctc gtccatgtgg gtcgggtact cggggtcgtc ggcgagcgtg
      601 accttgggcg gccgggtgag caggcagccg tagtagcagg cgaccttgag gccggcgaac
      661 ggccgggtga ccttggcggc gagctcctcg aggcccacgg cgtcgtggta gacgtcgatg
      721 aggttgtcga cctcgacgcc gccgttgtag gcgcggccga ccacggcacg cacgtcggcc
      781 gccgtctcct cgctctcggc gacctcgtgg gcgcccgtct tgaagcggct gtagcaggcg
      841 gcgcacggga cggccaccgc gtcgagcttc atgtcctgct cgaccttggc gagctcgttg
      901 accggcaggg ccacgctgag caggcggttg atggagtgcg ccgcggtgtt gccgcagcac
      961 tcccagcccg ggatctcctg gagcaccagg tcgagcgccc gggccgtcgc cctgaagctg
     1021 gcgtcgaact cgcccgacgt gccgtgcagc gagcagccgg ggtagtacgc aagggtcttg
     1081 gtcatgaccg gccccccgcg ttccgggcct cacgggcccg ggtgatgctc tcggcggtct
     1141 ggaccatacg cttcatggcc atcgtgtccc cgcgcttgag cgtggggttg atcttgccct
     1201 tgagcagcat cctgggaccg agcgtgaacc agttcttcat gctggtcggc cgcttgagga
     1261 cgtcgagcgt gatgagctcg acctcccaca tgcggcccca gcgcacgaac gagtcgtagt
     1321 tgagcttgag catctgggcc gacggggtgt cggcggggat gcggccctgc ttgcgcgcca
     1381 tgatcttgag ctcgtcgatg atggtcgcga tgtcgatgct ctgggggcag cgggtggtgc
     1441 aggtctcgca cccggtgcac agccagatga accggtcgtc gaagatcatg tcgaactgcc
     1501 cgagctggac ggcgcgcatg atcatcgccg ggtgcatgtc cgagtaccgg gcgaccggac
     1561 acccggacgt gcacttcttg cactggtagc acttgtaggc gttctcgtcg cagccggcgg
     1621 tgacctgccg gctgagcgag gcgctcggtg tgcaggtagc ggtcatgcgc gcctcctagg
     1681 cgaccgtggg acgcggcatg agtccggccg cctccacgat ctcgggcacg gcctcttccc
     1741 actcgaccgc catgatgtgc acgccggcga cgccctcgac ctcggccagt tccttgatca
     1801 tctcgacggc gagcttcttg ccctcggcgt gcgacgccgg gcccttctcg acgccggcca
     1861 tgcgctcgac gatatcgtcc ggcacgtcca tgcccggcac gaagttcttc atgtaccggg
     1921 cgccgccgac gcccttgagc ggcgtgaggc cggccatgat cttgatctcc tggtgcaggc
     1981 cctcggcgcg gacctcctcc atccacttct tgaacttggg gacgttgaag atgcactggg
     2041 tctgcatgaa gtcggcgccg gcgaggatct tcttcttgag ccggatggag cggaactcga
     2101 acgggtcgcc gaaggggttc tcggcgcagc cgatgtagaa cttcggcgcc acgtccatct
     2161 cgtcgccgca ctggaacttc ttctcgtcgc gcatgtcctt gagcatctgg atgaggttga
     2221 tggcgtcgag gtcgtgcacg cccttggcct cggggtggtt gccgaagctc atgtggtcgc
     2281 cggtgaggca gagcacgttg tgcacgccca gggtgtgggc gccgagcagg tcggcctgca
     2341 tggcgaggcg gttcctgtcc cggcaggtca tctgcatgat cgcctcgagg ccgtgctgct
     2401 gcacggtgac ggcggacggg agggaggcga tgcggacgac ggcggtctgg ttgtcggtga
     2461 tgttgaccgc atcgacgtag cccttgagca tgtcggcctt gccggcgatg acgccgccgt
     2521 cggcgctctt gggcggaccg cattcgccgg tgacggcgaa cttgccctcg ctgaagatct
     2581 tctcgaggtt ggtgccagcc ttgtactcag tcgcggtcat accggcttcg cctcctcgcg
     2641 caccatcttc cgcgggccgc cggagctgct gaggttccac ttcttcggcg gccggttctt
     2701 gatcatcagg tcctcacgac cctgctcctt gaggcgctcc cagatgaggt gccaggcgca
     2761 gtcgaggtcc tccttctccg gcgtcttgac ttcgcagagg ccgtcgtgcg agccgccgca
     2821 cgggccgttc atgaggctct tgctgcagcg cacgacgggg cagatgccgg cggtctcgcc
     2881 gatgacgcag tcgccgcagg cgccgcagcg ctccagccag acggcgtgct cctgcggcat
     2941 gcccagcatg ttggtgttga cggccggcac gacgagctgg ggcgagaact tctcggccat
     3001 gctctggatg ccgatgccgc aggccatgct gacgacgatg tccgcgtcct tgaccttgtc
     3061 ggcgatcatg tcgaggtact cccactcgca ctggcggatg gctgccgtgt gctcgatctc
     3121 ggcctcacgg ccgtcctgct cggccttgat ccgcagctcg ctggcgagga tctcagcctc
     3181 cttctcgccg cccgtgaggc agatggcgac acaggtgtcg cacccggcca cgaggacctt
     3241 cttcttgtcg ccgacgagcg cgaggatctc gtcgaggggt ttgcgttcgg cggtgatcat
     3301 tctgcgtgtc cttcctcact ggtggcggcg gacgtagcgg ggtcgtcggg catggccgcg
     3361 ccgccgagcg cttcgcgcgc ggcggcgtgg gcatccacac ccgcacgttt gacggggttg
     3421 gggccgagcg ccttgatgcg ctcggtgaac tcggtcgcga cctcggcgaa cttggcgccc
     3481 atggccgacg acatgttgta catctccacg cgctccggct cgatgccgac ctcggcgagc
     3541 aggtccttga cgtggttcac gcgcttgcgc gcccacaggt tgcccttgag gtagtggcac
     3601 tcgccctcga ggcagccggc ggcgtacacg ccgtccgcgc ccgcctcgat cgccttgagc
     3661 aggtggatga cctcgagctt gccggtgcac ggcagcttga tcatgcggat gttcgatggg
     3721 tactgcaggc gcatgctgcc ggccaggtcg gccgccgcat aggcgcagta gtggcacgcg
     3781 aacacgatga tctcgggctc gaagtcgggg tcgaccggct tcttcggcgc ctcctcggcg
     3841 gcctcgggag cggcttcgcc gccctcggcc accggggtct tcacgtcggc gtcggccatg
     3901 tcagctcacc tccatgaaga gcgcctcttc cttggcgaag acctgcgcgt cggtgtagtg
     3961 gtgcagcgtg atcgccttga ccgggcactc gctgacgcag acgccgcagc cctggcaggc
     4021 cgcagcctcg atcttggcct tcttggtctc gaggtcgatg atcggcacct cgtaggggca
     4081 gatgcgcacg cacgtgaggc aggctgcgca cttctccgtg tcgacctccg cgacgacgcc
     4141 gccggccttg agcaccgact tggccaggat ggacgcggcg cgtcccgcgg cggcgtaggc
     4201 ctgcgagatc gtctcttcga tgctcttggg gtagtgggcg gcgccgcaga ggaagatgcc
     4261 ctcggagccg aagtccacgg ggcgcagctt gacgtgcgcc tcgaggtaga agccgtcggc
     4321 ggtgagcggc accttgagca gcttgctcag ctcgctgccg cccgtggacg gcaccgtcgg
     4381 cgtggccagc acgagcaggt cgagcggaag ctccacgtcg cggcgcaggt acgggtcgcg
     4441 cgccttgacg ctgacgctgc cgttggcgct gacctccggc ttggagtcgt tgtcgtagcg
     4501 cgtgaagatg acgccgagct cacgcgccct ggtgtagtac tcctcgagga ggccgaacgt
     4561 gcggacctcc ttgaaccaga cgtacacctg gcgatccggg tcctgttcct tgagccggat
     4621 cgcgttcttg atgctctggg cgcagcaggt gcgcgagcag tacggcttgt tctcgtcgag
     4681 cgagccggcg cagaggatga agcccacgct cttgaccgcg gcgagcgccg ggtcgtgctc
     4741 cttgagcatg cgctcgaggt ccatcccggt gacgaccttc tggccggaac cgagaccgta
     4801 gagctcgggc ctctcctcga cgccgccggt cgcgaccacg acgacgccgt gatcgaccgg
     4861 cgtgcgcttg ccgtccttct cgccgatgat gctcgagaag ttgccgatga agccgtggaa
     4921 gtcggcgagc tcggcctcga ggtggatctt gacgttcggg ttgtcgacga ggcgctgctc
     4981 gagctgcgtg accagcgccg tcgggtcgtt gcccttgatg gtgtcgccga gctcgagcac
     5041 gtggccgccg agcttgggcg agcgctcgat caggtgtacc tcgtggccca tgtcgccgag
     5101 cgccagcgcg ctggtcatgc cggcgacgcc gccgccgacg accacggccg cgtgcgtgag
     5161 cgggacgtcg accttgaaca gcggctcgag gagccgggcc cgggccacgc tcatctggac
     5221 cagctccttg gccttctgcg tggccttctc cggctcgttg gcgtggaccc agctcgcctg
     5281 gtcgcggatg ttggccatct cgaagaggaa cgggttgagg ccggcctcgc gcagggtgtc
     5341 ctggaagatc ggctcgtgcg tgcgcggcgt gcagctcgcc acgaccacgc ggttcaggtc
     5401 gtgctcctcg atggcgtcgc ggatgagcga gaggctgtcc gaggagcacg tgtacatcgt
     5461 gtcggtggcg aacgccacgc cgggcagttc cttggcgtac tcggcgacgc tctcgacgcc
     5521 gatgacgccg gcgatgttgc tgccgcagtg gcagacgaag acgccgacgc gtggctccac
     5581 gccggccacg ttcttctcgg ccgggtactc cttgtcgacc gtgagcgtgt ggcgcgagtc
     5641 ggcgaggccg gtcatgacct gcgccgcggc ggcggatgcc tgggcgacgc tgtcggggat
     5701 gtccttgggc tcgccgaacg ggccgaccac gaacacgccc ttgcggctgg tctcgagcgg
     5761 gttgaactcg tgcagctcgc agaagccgtg ccggttgagg gcgacgccga ggtggccggc
     5821 cgcctcctgc gccttgcgcg caggctccag gccggccgag agcaccacca tgtcgtagcg
     5881 cgtctcgtgc agccgtccgc cctcgtcctc ccagctgatc agcaggtccc tggtcagcgg
     5941 gtcttccttg atgtagctcg ggcgcgaacg gatgaagccg acgcccttgt ccagcgcgcg
     6001 ctggtagaag gcgtcgaagc ccttgccctg ggcgcgcatg tccatgagga agacggtggg
     6061 ctcgcagtca ggctcgtggt cgatggtgag catcgcctgc ttgttggcga acatgcagca
     6121 gacgctggag cagtactcgt gctcctggtc gcgggagccc acgcactgga tgaaggcgat
     6181 cttcttggga tgagctccgt cgctgcggcg ctggacggag ccgatggtgg ggccgctggc
     6241 gctcagcatg cgctcgtact cgagcgccga gaggacgttg gggtagcgct ggtagccgta
     6301 ctcgcccttg tcgccggggt cgtggaggtc gaagcccgtc gccatgacca cggcgccgac
     6361 ctcgacgtcg atctcctcgt cgcgctcgcc gtgcagcagg gcgccggggc cgcaggccct
     6421 gacgcactcc atgcactcgg agcagatggc gcagtcgagg cagcgcttgg cctcggccac
     6481 cgcctgctcc tcggagaagc cggtgttgac ctcgtcccag acgatcttgc gcgccgcgcc
     6541 cggcaggtgc ggcatgggga ggcgcgccat gacggcggtc tgctcgagga cggcgtcctc
     6601 gggcttggcc tcgtccatga cgtcgtcctc gatggggagg aggtcttcgc cgcgcaggaa
     6661 gttgtggatg ctgcgcgccg cgcgacggcc cgcggcgacg gcctcgatgg ccgtccacgg
     6721 ggcgctggcc gcggcgtcgc cgccggcgaa cacgcccttc ttgccggtgc cgagcgtggc
     6781 cttgtcggtg aggatctggc ccttctcgac ggtgatgccc ttggacccct cgaggaagtc
     6841 gtcgaccatg ctctggccga tggcgaagat gacggtgtcg cactcgagct cgtgctccga
     6901 gccggcgatg gccaccggcg ggcgccagcc gcgctcgtcc gggtcgccga gcgagcaggc
     6961 gaggaactcg accttgctgg cgcggccgga ggcgtctgtg acgatctgcg agggcatgca
     7021 ggagcagttg aagctgatgc cttcgtccag gccctcggtg agctcgtcgt cggaggccgg
     7081 cacgctctgg tcgtcctcga tgcagttgat ggtgaccttc tcggcgccga gccgcagtgc
     7141 ggtgcgcccg gcgtcgaagg ccacgtcgcc gccgccgatg acgacgacgt tcttgcccat
     7201 gtcgaccttc tcgccgagcg aggcggccct gaggacgctg acggcgtcga ggacgccctt
     7261 ggcgtcggcg ccggggacgg gcagggtgcg gccgccctgg agaccgacgg cgaggtacac
     7321 ggccttgtag ccgccgccgt ccttcttcat gaggccgtcg acggtgaagt ccttgccggc
     7381 ggccttgtcg cactgcaggt cgacgccgag cgcgagcacg cggtcgatgt cctgctgcag
     7441 ggcggccttg ggcaggcggt attcggggat gccgaagcgc agcatgccgc ccggctccgg
     7501 cttgctctcg aagacggtga ccgcgtagcc cagcagggcg aggtcgcggg cggcggtgag
     7561 gccactgggg ccggcgccga ccacggcgac cttctccttg tacgtgacct cggccttctc
     7621 cggcagcggc acgttcgcga acgcgtagtc gctgacgaac cgcttgaggc cggcgatggc
     7681 gagcggctcg tcgacgtcac cgcgggtgca gtcggtctcg cacttgtgcg tgcagacgcg
     7741 gccgcagacg gcggtgaagg ggttggggtc gctggcgacg gtgtacgcct cggcgtagcg
     7801 gccgtcggcg atgagggcca ggtagccctg cgcgctggtg tgcacggcac aggcgcgctt
     7861 gcaggggctg tggcccttct tggtcacgaa gtacgtgttg gggatcgcct gcgcgtacag
     7921 cttggtgatg gctttgcggt cgttgaggcc ctcgttgtag ggatccggca ccgtgaccgg
     7981 gcacacaggg gagcactcgc cgcagctcgt gcagatatcc gggtcgacga accgggcgtg
     8041 gtggtagatc ttggcgtcga agctccccgg ctcgccctcg aggccgatga gctccgagtt
     8101 ggtgacgatc tcgatgttca ggtgcctgcc ggcctcgacc agcttgggcg ccagggtgca
     8161 catcgagcag tcgttggtgg ggaaggtctt gtcgagctgg gccatgtgac caccgagggc
     8221 cggcgccttc tcgacgaggt agaccttgta ccccccttcg gcaaggtcga gcgagcactg
     8281 gatgcccgcg acgccaccac cgacgacaag cgcagcaccg gtggatttgg gtggtgaacc
     8341 cgacatacgc actcctttgc gatacgcggc ggtcccaggt acgagtgggg tgaccagact
     8401 caacggccca cttgggtggc tcaattatag ggcccgcttg tgaccgcgtc cacacggccc
     8461 agacgcgcgt gcgaggcctc gtcgaggggg tcagcgcggg accggaccgg cgccatctga
     8521 taacctactg tcgcaaaagt caatcaagtg attaaccaag cgtcagccga aggggagtcg
     8581 atagcatggg cgtgcaggaa cagtaccgcg actacgtgat gacagggttc gtcaagcgcg
     8641 tcgagccggt ggtcgcggac catggcgacg gggcctacct ctacgacgcc gacggcaccg
     8701 agtacatcga ctgcttcgcc ggcatcagcg tcaccaacgc cggccaccac aaccagcaga
     8761 tcctggacgc cgccatcgcc caggccaaca agctcgtgca cgcctgcagc tacgtctacc
     8821 acgtcgagcc ggtcggcgcg ctcgcccaga agctcgccga ggtcacgccc ggcacgctgc
     8881 agaagacgtt cttcggcaac ggcggcgccg aggccatcga gggcgccttc cgcctcgcca
     8941 agcgctactc gggcaagcgc gagttcctgg ccatcgagtc gagcttccac ggccgcagct
     9001 tcgcgaccct ctcggtcacc gggaactccg gtcgcaagaa gggcggcggc ccgtacatgc
     9061 cgggcgtcgc gttcacgccc acgccgtact gctaccgctg cttcgccgag accacgccgg
     9121 acaggtgcga cctgctctgc gcgcgccgcg tcggcgagat cgcccgcctg cacctctcgg
     9181 gtgacgtctg cgccttcatc gccgagccgg tgatgggcga gggcggcatc atcgtcccgc
     9241 cggacggcta cttcaagcgt gtcaaggaga tcctcgacga gatgggcatc ctgttcatcg
     9301 ccgacgaggt ccagagcggg ttcgggcgca ccggcaagct gttcgccatc gaccactacg
     9361 gcgtcgagcc ggacatcatg acgctggcca agggcatcgc cgacggctgg ccgctgtccg
     9421 ccttcatcgc caagcccgag atcgccgacg ccttccagat cggcgaccac ctcagcacgt
     9481 tcggcggcaa ccccgtgagc tgcgccgcgg cgctcgccaa catcgacttc ctcctggacg
     9541 gggtcgtcga gggcgcggcg cgcaagggcg aggccctgat ggcgaagttc cgggagctcg
     9601 ccaagacgca gccgctcatc ggcgacgtgc gcggcaaggg cctcatggtc ggcgtcgagc
     9661 tcatcgccga cccggcgacc aaggcctacg gcaccgccca ggccggcttc gtgcgccagt
     9721 actgcctcga ccacggcgtg ctcatcggcg tgggcggcaa cttcggcagc gtgctgcgca
     9781 tccagccgcc gctcgtcatc gacgacgccc agctcgacag ggtgttcgcg acggtggccg
     9841 acgccctcga ggcggcaccc aggcagtgat cggctgagag agaccagatg agccgggccg
     9901 ggaggcccgg cgtccgcggc ggccgtcggg gacgtcgccg cagccgtcgt gaggggcggg
     9961 aggcccgcac gtggcaaggg ccgcccgccc cacacgcgtg
//
LOCUS       DASUMX010000165        22671 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_489194, whole genome shotgun sequence.
ACCESSION   DASUMX010000165 DASUMX010000000
VERSION     DASUMX010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 22671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22671
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489194"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="VFZ86_08250"
     CDS             complement(<1..187)
                     /locus_tag="VFZ86_08250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642314.1"
                     /translation="MAGFEPAPRGMLTERSEWSAAQIIRVTAIVTLTVLAIVGVLEFL
                     SQVRTILLWVLIGVILAV"
     gene            282..358
                     /locus_tag="VFZ86_08255"
     tRNA            282..358
                     /locus_tag="VFZ86_08255"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:316..318,aa:Ile,seq:cat)
     gene            complement(519..1385)
                     /locus_tag="VFZ86_08260"
     CDS             complement(519..1385)
                     /locus_tag="VFZ86_08260"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5642315.1"
                     /translation="MSLPKRVLVTGASGNVGREVVRDLRGREIEVRAAVPDEKAAQGV
                     PKDVPWVPFDFTSPDTFDPALEGVDRVFLMRPPHMSDAAAFEPFLAAMKQADVRQVTF
                     LSLLGVEKNPIVPHHGIEKGLRASGLGWTMVRPAFFMQNLTTTHLADLREGRIVVPAG
                     AGRTSFIDVRDIGAVAALTLAEVGHLGKAHSITGREALTYAECAALLSEASKREIVYT
                     NPSGREFARHMADQGFPPDYIRVMRGIYLVCKLRLAGRITDELPRLLGRPAISFAQFA
                     QDHVGLIDEPLS"
     gene            complement(1771..2268)
                     /gene="arr"
                     /locus_tag="VFZ86_08265"
     CDS             complement(1771..2268)
                     /gene="arr"
                     /locus_tag="VFZ86_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018621553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)--rifampin ADP-ribosyltransferase"
                     /protein_id="HEX5642316.1"
                     /translation="MASANEGPFVVSEPCPVCGGQGAINGQPCLPCHGTGVQRFFHGT
                     KADLEPGDLIGPGHRSNFGQRKQGTYAYLTGTLDAAVWGAELALGEGPGRIYVVEPTG
                     PIMDDPNLTDKKFPGNPTKSYRSREPLRVTGEVKEWQGHSPEALKAMRDNLERLRRQG
                     VEPID"
     gene            complement(2524..4695)
                     /gene="katG"
                     /locus_tag="VFZ86_08270"
     CDS             complement(2524..4695)
                     /gene="katG"
                     /locus_tag="VFZ86_08270"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF011635.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HEX5642317.1"
                     /translation="MSDASSSPVNTPAATRVREWWPEQLDLTVLHRNPPAADPMGPDF
                     DYAAEFATLDLEAVRRDLMSLMTDSQEWWPADYGHYGPLFIRMAWHSAGTYRISDGRG
                     GGGLGAQRFAPLNSWPDNGNLDKARRLLWPVKRKYGRKISWADLIILAGNCALESMGF
                     MTFGFGGGRADVWEPDDIDWGAEKAWLEDKRYGGDRELANPLAAVQMGLIYVNPEGPN
                     GTPSALAAARDIRETFRRMAMNDEETVALIAGGHTFGKTHGAGDAANVGPEPEAAPLQ
                     QMGLGWKSGHGTGSGNDTITSGLEGAWTPTPTSWDNSFFETLFRYDWDLTKSPAGAWQ
                     WVPTDPSAADTVPDPHDPTKRHAPVMLTTDLALRMDPIYEPISRRFLENPQEFAEAFA
                     KAWFKLTHRDMGPITRYLGPLVPAEPQIWQDPVPPVSHPLVGAQDIAALKGTILASGL
                     SVSQLVSTAWASASTFRGTDKRGGANGARIRLEPQGGWAVNEPAKLAQVLRALETIQQ
                     EFEAQAVGGKRISLADLIVLGGCAGVEKAARDAGFEVEVPFTPGRTDTTQEQTDVESF
                     AVLEPTADGFRNYQGDGQLKPAEQLLVEKATLLTLSAPEMTVLVGGLRVLGANHDGSE
                     LGVLTARKGELTNDFFVNLVDMATTWEPSDETEATFEGRDRSTGEARWTASRVDLVFG
                     SNSQLRALVEVYAGDDAQEKFVRDFVAAWDKVMMLDRFDVA"
     gene            complement(4756..5217)
                     /locus_tag="VFZ86_08275"
     CDS             complement(4756..5217)
                     /locus_tag="VFZ86_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014261374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEX5642318.1"
                     /translation="MSARVDEVTRRVDLLSQGLRDAGLRLTHQRLEIVRVVAADETHP
                     DVETVFRSVRQRVPTISLDTVYRTLSTLTERGLIVRVLFTPGPARYDANPARHHHFVC
                     TRCGLIRDVEDEDLDAIRPTAEVARIGRPETVSVQFRGVCAACRSKEETTA"
     gene            5446..6195
                     /locus_tag="VFZ86_08280"
     CDS             5446..6195
                     /locus_tag="VFZ86_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010422613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HEX5642319.1"
                     /translation="MTDVQGKSVEVCFSPGLFPDILTKDDFVVVLVDILRATTTICTA
                     VANGVEAIIPVATHEEARRLKAEGYLVASEKDGVQLDFADFGNSAFSFTRDRIGGRML
                     VYCTTNGTRALKIAESAGGIAIGAFVNISALTEWLARRRQNVVVLCSGWKDRFCLEDT
                     LFAGALTGRLLATGLFRTECDSAHASMDLWSLAEGDVLGYIEKAAQRHRLKRLELDDV
                     IPYSFACDQVAVVPVFDGGVIKDAAHTSREG"
     gene            6192..7604
                     /locus_tag="VFZ86_08285"
     CDS             6192..7604
                     /locus_tag="VFZ86_08285"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX5642320.1"
                     /translation="MRQVSDTGLEKILGDLYPYRSTTETFRRVPPEPVDKDSILAEVA
                     GLSHAEDALGDKGTVSGSIYAGDHAHYHFLGQVFDQFSHANVLQRDMYPSATKFEGEI
                     IAMTSDLLHGTGVGVVTSGGSESLMTALYAYREQARARGVTRPNLVMPVTAHCSLDKG
                     AHWMDVEVRHAPLTDGYLADVAAMADLVDDQTVALVGSAGNYAHGLIDPIEELAELAR
                     SRGIGLHVDGCLGGWLLPWVERLGYEVPPWDFRVPGVTSISADTHKYGYALKGSSVLL
                     YRDKELRKHQYFTYPTWPGGLYVSPGFAGSRSGGILASTWAALVATGESGYLAAADAI
                     MRTATTIKEGIRDGIPELEVIGDPLFLIAFKAADDLDIYLVNDSLIAQGWRMIALQLP
                     PALHFCITRPNARPGLADAYLEALHTAVAYARDNKGKKALSGAVYGFGGTALGTEMLK
                     FAMARFLDAMHDLPDEPGAA"
     gene            7604..9208
                     /locus_tag="VFZ86_08290"
     CDS             7604..9208
                     /locus_tag="VFZ86_08290"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013828483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HEX5642321.1"
                     /translation="MSEPWVLAVDLGNGGPKVAVVSLEGEVLRVAFRPVHARIGLDGA
                     ATQDAVEWWAGLRSAAREAISGVGADGDRLHVVAITGQYGSSVPVGAGGEPVGEVLLW
                     ADTRARELAREVIGGPLNIGGFAPHKVLPFVRITGGAPSPSGADPTGHALLLRERLRE
                     VYEKTRVILEPVDYLGLRFTGRAAATPASMLASWLTDNRIGAPFGYVDELLVRARREK
                     SKLPELLPTGAVLGPLLPDVAEELGVAAGVPVVCGVPDLHAAVIGSGAIAPYDTHVAI
                     STTAWISSRVPFKRTDILHQIATVPGIDAAHPIVVNNQETGGAALHWLREQIVAPNDG
                     LMGGGSGIGAEGAAAEALAPSFDDLIRLAAQAPAGCEGLLFTPWLNGERSPAEDKVVR
                     AGWLNLSLRSDRAMLVRSVLEGVAYNARWLFDAYEKFLRRSVPKVRILGGGAQSELWC
                     QIYADVFGRPVEQVADPRNAQLRGVALWARICLGELTLDDVPGLVPVPESFVPTDAAA
                     VYARGYAEYRKLFGRLKGLYHRLNSG"
     gene            complement(9221..10348)
                     /locus_tag="VFZ86_08295"
     CDS             complement(9221..10348)
                     /locus_tag="VFZ86_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5642322.1"
                     /translation="MPEPAPADETTAPSVALIDCAEVTCLIEEGHELGCLSSGDVATA
                     LRDVEVTATQLEELLIVLAEMGIEIIEDDARAETDAAGEAAEDGDAGAKLDLSVKAVS
                     SDPVRLYFHEMGKVPLLTGVEEVALARRIERGDAAARRKLIESNLRLVVSIAKRHAGR
                     GMPLLDLIQEGNLGLMRAVEKFDYRRGFKFSTYATWWIRQAITRALADQSRTIRVPVH
                     MVENIQTYVRVQRLLIQEIGREPVPEEIAAEMGVTVAKIREIQRISQEPASLHQQVGD
                     EGDSQLGDFVEDTKSAAPVEEVAEIVRHEEIMAVLGLLSRRERTVLRLRFGLDDGRPR
                     TLEEVGQVFGVTRERIRQIEARTLAKLKAIREAQCLQEYLE"
     gene            10715..10921
                     /locus_tag="VFZ86_08300"
     CDS             10715..10921
                     /locus_tag="VFZ86_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX5642323.1"
                     /translation="MATGKVKWFNAAKGYGFIEQADGDDLFVHFSEIQSEGFKSLDEG
                     QAVSFEAGTGKNNKPQATNVRVIS"
     gene            complement(10989..11381)
                     /locus_tag="VFZ86_08305"
     CDS             complement(10989..11381)
                     /locus_tag="VFZ86_08305"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5642324.1"
                     /translation="MPQGRVRVLIVDDHELVREGLKALLSRDPAIDVVGEASTGLEAL
                     ELVPRVLPDVALMDLRMPVMDGAEATARIRDAYPRVQVVILSGAGDSALAKAALEGGA
                     VSYLIKDARHDALTRAIHEAASGAAGTP"
     gene            complement(11722..13053)
                     /locus_tag="VFZ86_08310"
     CDS             complement(11722..13053)
                     /locus_tag="VFZ86_08310"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX5642325.1"
                     /translation="MPGYDVVVIGSGTAAQNVAPRCAQEGLRVAVVDRLAYGGTCGQR
                     GCDPKKVLLAAAEAVSRARGLDGRGLTGPPTIDWPALIARKRTFTEPLPERIEGWMRD
                     AGAETLHGTARLRSAHEVQVDGRTLEAAEVVVASGARPMDLGIPGQELVTTSTGFLEL
                     GDLPPRVVFIGGGYITFEFAWLARMAGAAVTILHRSAHVLKGFDKQLADALVERYRSL
                     GIDVLTEAPVNEVTRRGDELVVAHETGEVAADLVVHGAGRVADLDDLGLESAGIAAGP
                     RGVRVDEHLRSVDDAHVWAAGDAADKGLPLTPVASKEGKVVAAGILGEDAVYDGRAVP
                     SVCFSDPPLAAVGMSTAEAQGRGDAVHVERADASEWFSQRRVGQTHAGMAVVTDAASG
                     RLLGAHLLGVNADEVVNVLALAVRHGLTAADLTAMTWSYPTATSDLPYVLG"
     gene            13335..14099
                     /locus_tag="VFZ86_08315"
     CDS             13335..14099
                     /locus_tag="VFZ86_08315"
                     /inference="COORDINATES: protein motif:HMM:NF012669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HEX5642326.1"
                     /translation="MPAERRARIVSSLKDQRAVRVPALSEDLGVSEITIRRDLLLLEQ
                     EGVLDRTYGGAVAKKQLAAESRWEEAAAVNAAQKDAIAAAAAEMIEPGDTVFFGSGTT
                     VVGILRHVDPELEARVVTHSLAVAAEARGLSLEVLFLGGLYRPQLDAVEGTWPLDMIN
                     QVYADKAFLGADGLDVRAGLTSPSMAAATVELAMIRRTRGEVVVLADSSKIGLVGPLV
                     ICPLDQIDVVLVDDGIDPAVVKQIQRGGPQCEVVPA"
     gene            14125..15066
                     /locus_tag="VFZ86_08320"
     CDS             14125..15066
                     /locus_tag="VFZ86_08320"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline/ethanolamine kinase family protein"
                     /protein_id="HEX5642327.1"
                     /translation="MDEVASVEHLTPEAAIARIPDWNGLDVQVETLGGGITNHNYVVT
                     VSGRPDLPWGGKYVMRIPGQGTDRFIDREREHRNAVAAAEAGVTPPILHMLEPEMCTV
                     VPFIEGETMHPEDLAGHPDRLEKVVDVMRAYHDKATFANDIHVFDMIREYLDMARGVD
                     ATFPDDMDWMLGLGDRIEEAMKRDQPAPVACHCDLLSENFILEPGGRMWVIDWEYGGM
                     ADPYFDLGDFCVEHPLSDDEERFIITRYCGSFSEERYARMMLHKIVADLWWSIWAWIQ
                     VRLSKIDFDFQDYGMNRVARLRANAARPEFESWLATV"
     gene            15085..15897
                     /locus_tag="VFZ86_08325"
     CDS             15085..15897
                     /locus_tag="VFZ86_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642328.1"
                     /translation="MNDDGTRPAGEMGPDVGMGKVDPEMARTFQEEAVRVVRAGEEQG
                     VHLRVIGALAFWHHCPEFGYLQEKLNRVYTDIDFAGYGKQDQQIRKLFVSIGYDEDLE
                     INAFHAEGGRLIFNNPTNHIHVDVFMDKLDFCHPIPWKGRLEVDSPTIPLAELLLEKM
                     QIVKINEKDIIDTIMLLLEHEICGDDEDHINSQHVAKLCAGEWGLWRTTTMNLEKVRD
                     YLPHLDLDEAHKQIVTTRVAELMSAMDDYPKGTRWKLRARVGDKVKWYKEID"
     gene            15912..16913
                     /locus_tag="VFZ86_08330"
     CDS             15912..16913
                     /locus_tag="VFZ86_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642329.1"
                     /translation="MGLFSRKGGSSDDRVRIFFATDVHGSERTYRKFVNGAKFYKVDH
                     LVMGGDIEGKFLVPIVAEGGGHYRVTLQEDISHLDGDDELKALKERIETLGFYHVVVT
                     EEEIREMQADQSLVDAAFKREAHARLERWIALADERLAGTDTMMYVTGGNDDPQELID
                     TLEEKQSDHVINCEGRIVMLSDTFPMANCGYSNPTPWNTPRETDEETLEKYLEESLQG
                     IDDFTSAIFNFHVPPYDSTLDDCPKLDWSTDPPAPVVVAGVPQSAPAGSHAVRNVVER
                     HQPLLMLTGHIHESRGLVKVKRTTVVNPGSEYGEGILRGCILTLAPGKVVGYQMTSG"
     gene            16979..18613
                     /locus_tag="VFZ86_08335"
     CDS             16979..18613
                     /locus_tag="VFZ86_08335"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX5642330.1"
                     /translation="MATTAAKPEVFSRKASGLSRVMSPWSAFMYNFLTMGVIFPWTFV
                     WAPAAFPGVSVWKACLLAILLELPIALAYVWMATAMPRSGGDYVFQSRVLGGAIGFPV
                     VMSGFVIWILQWVALAGWLLATLGVAPLFMGLGVYYENQGLIDAAVWAQGSQGVVTIS
                     IIGAAAMMILLVTGFKNYVRLQYAMFAATGILVVILLVQFLRTTPAEFAVAMNHFSNV
                     VDGRTDFYRWLQKDVADAGVNLLPAITFGAILLATPIVWTSLQWATYSVQQGGEIKGA
                     RVFKNQMFIIIGSMIAVGVVLALIAWAEERAVGTAFFNAVSHSYYYVVSASGDGIGSV
                     LPFPGMFAIVISPNPIIVILVAVSFMLASLQITCNCYIGMTRIMVGMSLDRTLPTWFS
                     KVSPRFRSPVNAHLIYFALGCVAILGYNYWTQWYSMTLGVTLACGYVFTISCLAAALL
                     PFRAKALYEASPGAKYTLGGFPLVTIFGIIGFVFGLWALIMFLTNSAYGLTGTTPYIV
                     VAGIFVVCLIGYWIGRAYNKGKGIDVNYAFLEVPPE"
     gene            18815..20023
                     /locus_tag="VFZ86_08340"
     CDS             18815..20023
                     /locus_tag="VFZ86_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5642331.1"
                     /translation="MGTPETIAVESAHMPPFFAKTPISIARGEGVWVWDEEGRRYLDL
                     TAGWGVTSIGHAHPVIVRALAEQGARIIQNPDSGLTYSPARARLLELLAGILPAGLTR
                     VFFTNSGAEANDAAVKLARKATGRTEVIATEGSFHGRTISMVSATGQARHRDKFRPQM
                     PGYRFVPYGRADAVAAALGADVAALIVEPVQGEGGVRIPAPDYLRELSRLCRANGSLL
                     VVDEVQTGFCRTGPLFATAAAGAEADFLTMAKGIAGGFPLGAFAMTDAVAGRLEAGDH
                     GGTYCGNPLACAVAEAVIRHLIDEGVAARVEELGGEALARMGSWRERFPETVAGARGA
                     GLLLIVELTGEEAAAAVAAECLRRGAFVRQTQGNGIRVFPALTITRAELDQGLTVLEE
                     SIAAVADRAG"
     gene            20045..20923
                     /locus_tag="VFZ86_08345"
     CDS             20045..20923
                     /locus_tag="VFZ86_08345"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX5642332.1"
                     /translation="MAQVSVLGLGAMGSTLARVLLERGHRVTVWNRSDSERAADVAAA
                     GAARAATPAAAVSAAPLVLMCVTDYPAAGDVLAAPGALAGLAGRTLVQLTNGNEEQVR
                     AQMARVRAAGGRMLAGGILGYPRHIGGRETVIMYAGDAEAFEEHAATLAGLAGAQRFS
                     GADPGPPNAAYVAAFGFYFAALAGFLEAAALAASRGVAPAEFAAAMPGMTALLLDHID
                     DAARRIEDDDYAGDQATVDVHLVGSERRRRTLADAGLASLMTGAFVEYCRRAQAAGEG
                     GEDVAALFKRMRAPSP"
     gene            complement(21062..21193)
                     /locus_tag="VFZ86_08350"
     CDS             complement(21062..21193)
                     /locus_tag="VFZ86_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642333.1"
                     /translation="MTTSGYNPTAMPGPETRYIPHIPDFTPRSTDGIVTDGSKTEGD"
     gene            complement(21397..21966)
                     /locus_tag="VFZ86_08355"
     CDS             complement(21397..21966)
                     /locus_tag="VFZ86_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016456587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5642334.1"
                     /translation="MRVLIAEDEALIRMDLREMLEEEGHEVVGEARDGAEALTLARAT
                     RPDIVFMDVKMPNVDGIEAASAISAESIAPVVMVTAFSQAGLVEEAARAGAMGYVVKP
                     FSRNDIVPAMQVAVSRHAEMSLLAQQVGDLEERLETRKLLDRAKGLLMAKGLSEPEAF
                     HRLQKLAMDKRKSLREVAEAVVTAHEAME"
     gene            complement(21989..>22671)
                     /locus_tag="VFZ86_08360"
     CDS             complement(21989..>22671)
                     /locus_tag="VFZ86_08360"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX5642335.1"
                     /translation="GALVLVEDLTEARRRERELGVKEATIREVHHRVKNNLQTIASLL
                     RLQARRSGSDEVRRALAEAVGRAAAMATVHDMLAHSAEECVDFAEVARRVVGLVRSSV
                     VGDDPRFAVRVSGVTGPVDAGPATSLALVLTELVHNSLEHAFGPVDRGTVSVDLSRDA
                     EGLMLIVRDDGRGLPADFDPATSKGLGLSIVRSLVEEDLGGTLSLSPGEGAGIAIRVP
                     LGRGGDSL"
BASE COUNT         3335 a         7924 c         7936 g         3476 t
ORIGIN      
        1 ccaccgccag gatcacgccg atgagcaccc acaggaggat ggtgcggacc tgggagagga
       61 actcgaggac gccgacgatc gcgagcacgg tcagcgtgac gatggccgtg acgcggatga
      121 tctgggccgc actccactcg ctgcgctccg tgagcatgcc gcgcggcgct ggttcgaacc
      181 cggccaccac gtcacctccc cctgtgcgtc ctgctgccga ctgcgtcccc gagagcgtat
      241 cattgggcgg cgccggcgcg gcgtcccgcg ccgccccgca cgggcctata gctcagttgg
      301 tcagagcagc cgactcataa tcggccggtc gcaggttcga gtcctgctgg gcccaccacg
      361 ttcttgttgt cgtgttccgg gtgacactgg acgcttgaag gagcttgctg ccacgtgatg
      421 ggagagaggg ctcctgccac catgaggaag ccgatcacgt cgggcatgag tcgcgtagca
      481 agggtgcttg tcgaccgaca gaagaggcga aggaccagct aggagagcgg ctcgtcgatc
      541 aagccgacat gatcctgtgc gaactgcgcg aagctgatag ccggacgccc caggagtcga
      601 ggtagctcgt cggtgatgcg tccggcgagg cggagcttgc agaccaggta gatgccgcgc
      661 atcacgcgga tgtagtcggg cggaaagccc tggtccgcca tgtgtctggc gaactcgcgt
      721 ccactggggt tggtgtagac gatctcgcgt ttgctggcct cactgagcaa agcggcgcat
      781 tcggcatagg tcagcgcttc acggccggtg atcgagtggg ctttgccgag gtggccgacc
      841 tcggccagag tgagggctgc cacggcgccg atgtcgcgaa cgtcgatgaa gcttgtgcga
      901 ccggcgcctg ccgggacgac gatgcgcccc tcgcgcagat ccgcgagatg agtagtggtc
      961 aggttctgca tgaagaaggc cgggcgtacc atcgtccagc cgaggccgga agcccgcagg
     1021 cccttctcga tgccgtgatg cggcacgatg gggttcttct cgacgccgag cagcgacaag
     1081 aaggtgactt gccgcacatc ggcctgcttc atggctgcca ggaacggctc aaaagctgcg
     1141 gcatcggaca tgtgcggcgg tcgcatgagg aagacgcggt cgacgccctc gagggcggga
     1201 tcgaacgtgt ccggggacgt gaaatcaaac ggcacccagg gcacgtcctt cgggactccc
     1261 tgggcagcct tctcatcagg cacggcggca cgcacttcga tctcgcgacc gcgcaggtca
     1321 cgcaccacct cgcgcccgac gttgccgctg gcgccagtga caagcacgcg cttgggcagg
     1381 ctcatcggtc cacctcgcgt gactcactgg tctcaagtgc gacgcagatg cgctcgagca
     1441 gagcgaagag gtgattgcgt tcgtcgctgg acaggtcgtg caggcgagtc atgcgcttca
     1501 tgctgggttc ggcgatcgac gacatgcgag agatggagcg cctggtcaag acgagtgaaa
     1561 cggtctggac gatcgctcga cccggtggct tgaacaatca gccaaaagga caatacgtca
     1621 tctctcctgg caacgcactc aagggacacg gccggactcg tcgtgccgac ctcgccgaag
     1681 tcctcgtgct ggcggtgtcg gaggagtgtt ggccagggca ggccgtggtg gtggcaagtc
     1741 gatgcgctga tgccacagtg tcagatcgag tcagtcgatc ggctcgacgc cttgccgcct
     1801 caaacgctcg aggttgtccc tcatggcttt gagcgcctcg ggggagtgcc cctgccactc
     1861 cttcacctcg cccgtgaccc ggagcggctc tcgggagcgg tacgacttgg tcggattgcc
     1921 cgggaacttc ttgtcggtca gattggggtc gtccatgatc ggaccggtcg gctccacgac
     1981 gtagatcctg ccggggcctt cgccaagcgc cagctccgcc ccccagacgg ccgcatccag
     2041 ggtgccggtc aggtacgcgt aggtcccctg cttcctctgg ccgaagttgg agcggtggcc
     2101 cggcccgatc aggtctcccg gctccaggtc ggccttcgtg ccgtggaaga accgctgcac
     2161 acccgtgccg tgacacggga ggcagggctg cccgttgatg gcgccctgcc ctccgcagac
     2221 ggggcacggc tctgacacga cgaatgggcc ctcgttggcg gatgccatcg gctgactcct
     2281 ctcgagactc gatccttgtc ttcccaccac gcggccgttc agtgcctgtt ccagaccgag
     2341 tgtgattcgg atcacgcttc gatggacctg tggcgcctgg ccaggggcca cctgggctac
     2401 atcgagaagg ccgccgaagc gcaggaggct cgcggcgccg cgggctgccg aggccgcggc
     2461 gtgaagaggg ggccggtccc gctggaccgg ccccctctca cactcgcacc catctcggct
     2521 cgctcaggcg acgtcgaacc ggtcgagcat catgaccttg tcccacgcgg cgacgaagtc
     2581 gcgcacgaac ttctcctgcg cgtcgtcgcc ggcgtagacc tcgacgagcg cgcgcagctg
     2641 ggagttcgag ccgaagacca ggtcgacgcg gctggcggtc cagcgagcct cacccgtcga
     2701 ccggtcgcga ccctcgaacg tcgcctccgt ctcatcgctc ggctcccacg tcgtggccat
     2761 gtcgacgaga ttgacgaaga agtcgttggt cagctctccc ttgcgcgcgg tcagcacgcc
     2821 gagctccgac ccgtcgtggt tcgcgccgag cacgcgcagg ccgcccacga gcaccgtcat
     2881 ctccggcgcg ctgagcgtga gcagggtcgc cttctcgacg agcagctgct cggcgggctt
     2941 gagctgaccg tcgccctggt agttgcggaa cccgtccgcc gtcggctcca gcacggcgaa
     3001 cgactcgacg tcggtctgct cctgcgtcgt gtccgtgcgc ccgggggtga agggcacctc
     3061 gacctcgaag ccggcgtctc tcgccgcctt ctcgacgccg gcgcacccgc ccagcacgat
     3121 caggtcggcg agcgagatgc gcttgccgcc gaccgcctgc gcctcgaact cctgctggat
     3181 cgtctccagc gcgcgcagca cttgcgccag cttcgcgggc tcgttgacgg cccagccgcc
     3241 ctgcggctcg agtcggatgc gcgcgccgtt ggcgccgccg cgcttgtcgg tgccgcggaa
     3301 cgtcgacgcc gaggcccacg cggtcgagac gagctgagac accgagagcc cggaggcgag
     3361 gatcgtgccc ttgagggcgg cgatgtcctg cgctccgacc agcgggtggg agaccggagg
     3421 gaccgggtcc tgccagatct gcggctcggc cggcaccagc gggccgaggt agcgcgtgat
     3481 cggccccatg tcgcggtgcg tgagcttgaa ccaggccttg gcgaaggcct cggcgaactc
     3541 ctgcgggttc tcgaggaagc gccgggagat gggctcgtag atcgggtcca tccgcagcgc
     3601 gaggtcggtc gtgagcatca ccggcgcgtg ccgcttcgtc ggatcgtgcg ggtccggcac
     3661 ggtgtccgcc gcggacgggt ccgtcgggac ccactgccac gctccggccg ggctcttggt
     3721 caggtcccag tcgtagcgga acagcgtctc gaagaagctg ttgtcccagc tggtcggcgt
     3781 gggggtccag gcgccctcca gcccgctggt gatggtgtcg ttaccactcc cggtgccgtg
     3841 gccgctcttc cagccgaggc ccatctgctg cagcggcgcc gcctcgggct cggggccgac
     3901 gttcgcggcg tcgccggcgc cgtgggtctt gccgaacgtg tgccctccgg cgatcagcgc
     3961 caccgtctcc tcgtcgttca tggccatgcg gcggaacgtc tcccggatgt cccgggccgc
     4021 ggcgagcgcg ctcggggtgc cgttcggccc ctccgggttc acgtagatca ggcccatctg
     4081 gacggcggcc agcggattgg cgagctcgcg gtcgccgccg taccgcttgt cctcaagcca
     4141 cgccttctcg gcgccccagt cgatgtcgtc cggctcccac acgtccgcac ggccgccgcc
     4201 gaagccgaat gtcatgaagc ccatcgactc cagggcgcag ttgccggcga ggatgatgag
     4261 gtcggcccag gagatcttgc ggccgtactt ccgcttgacc ggccacagca ggcggcgtgc
     4321 cttgtcgagg ttcccgttgt cgggccagct gttgagcggg gcgaaccgct gcgcgcccag
     4381 gcccccgccg ccgcggccgt cgctgatccg gtaggtgccg gcgctgtgcc aggccatgcg
     4441 gatgaacagc gggccgtagt gaccgtagtc ggccggccac cactcctgcg agtccgtcat
     4501 cagggacatg aggtcgcgcc ggaccgcctc gaggtccagg gtggcgaact cggcggcgta
     4561 gtcgaagtcc ggtcccatcg ggtcggccgc cggcgggttg cggtgcagca ccgtcaggtc
     4621 gagctgctcg ggccaccact cccggacccg cgtcgccgcg ggcgtgttga ccgggctgga
     4681 gcttgcgtcg gacatgtcag tctctcctct cgaggcggcg gggcggccgc ggggacaacg
     4741 ggcgagcgtt ggcggtcatg ccgtggtctc ctccttgctt ctgcaggcgg cgcagacccc
     4801 gcggaactgg acggataccg tctcggggcg cccgatacgg gcgacctccg cggtcggtcg
     4861 gatggcgtcg aggtcctcgt cctccacgtc ccggatcaga ccacatcgcg tgcagacgaa
     4921 gtggtggtgc cgggccgggt tggcgtcgta gcgcgcgggt cccggcgtga acaggacgcg
     4981 cacgatgagc ccgcgctcgg tgagcgtgga cagcgtgcgg tacacggtgt cgagtgagat
     5041 ggtcgggacc cgctgccgca cggaccggaa gacggtctcg acgtccgggt gcgtctcgtc
     5101 ggcggcgacg acgcgcacga tctcgaggcg ctgatgcgtc agccgcagcc cggcgtcgcg
     5161 aaggccctgc gacagcaggt cgacgcgacg cgtcacctca tcgactcgcg cgctcactgc
     5221 actcctatcc gtcgaagaca cctggagggc ctcaccggcc gccggacgcg cttcttacag
     5281 tagcattctt atataggagc gagacggccg gaacaaccgt tggacggcct tcgtctgaga
     5341 gacggctgcc tcagtctgcg cacgacgcgt ctgcccgcgg cccgtggacc gccgtcgccc
     5401 catcggctag ggtgaccatc caggctgttg tcgcggcggg aaaggatgac tgacgtgcag
     5461 gggaaaagcg ttgaagtctg cttctcgccc gggctgttcc cggacatcct gaccaaagac
     5521 gacttcgtag tcgtgctggt ggacatcctg cgcgcgacga ccaccatctg caccgcggtc
     5581 gcgaacgggg tggaggcgat catccccgtc gccactcacg aagaggcacg acggctcaag
     5641 gccgaggggt acctcgtcgc gtccgagaag gacggcgtgc agctcgactt cgcggatttc
     5701 ggcaactcgg ccttcagctt caccagggac aggatcggcg gaaggatgct ggtctactgc
     5761 acgaccaacg gtacgcgggc gctcaagatc gcggagagcg ccggcggcat cgccatcggg
     5821 gccttcgtca acatctcggc gctgacggag tggctcgccc ggcggcggca gaacgtggtc
     5881 gtcctgtgct cgggctggaa ggaccggttc tgtctcgagg acacgctctt tgcgggagcc
     5941 ctgacgggga gactgctggc gacgggcctg ttccgcaccg agtgcgactc ggcgcacgcc
     6001 tcgatggacc tctggagcct ggccgagggc gacgtgctgg gctacatcga gaaggctgcc
     6061 cagcgccatc gcctgaagag actcgaactg gacgacgtca tcccgtactc gttcgcgtgc
     6121 gaccaggtgg cggtcgtgcc agtgttcgac gggggcgtga tcaaggacgc ggcacacacg
     6181 agcagggaag ggtgagacag gtgagcgaca cgggcctcga gaagatcctc ggcgacctct
     6241 acccgtacag gagcacgacc gagaccttcc ggcgcgtgcc gccggagccc gtcgacaagg
     6301 attccatcct ggccgaggtc gccggcctgt cccacgccga ggacgcgctc ggcgacaagg
     6361 gcacggtgtc gggcagcatc tacgccggcg accacgccca ctaccacttc ctcggccagg
     6421 tgttcgacca gttctcccac gccaacgtcc tgcagcgcga catgtacccg agcgcgacca
     6481 agttcgaggg cgagatcatc gccatgacct ccgacctcct gcacgggacg ggcgtcggcg
     6541 tcgtgaccag cggcggctcg gagagcctga tgaccgcgtt gtacgcgtac cgcgagcagg
     6601 cgcgcgcccg cggcgtgacc aggcccaacc tggtcatgcc ggtgaccgcg cactgctcgc
     6661 tggacaaggg cgcgcactgg atggacgtcg aggtgcggca cgcgccgctc accgacggct
     6721 atctcgccga cgtcgccgcc atggccgacc tcgtcgacga ccagacggtc gcgctggtcg
     6781 gcagcgccgg caactacgcg cacggcctca tcgacccgat cgaggagctc gccgagctgg
     6841 cgcggtcgag gggcatcggc ctgcacgtcg acggctgcct cggcggctgg ctgctcccgt
     6901 gggtcgagcg cctcggctac gaggtgccgc cgtgggactt ccgcgtcccg ggcgtgacct
     6961 cgatctctgc cgacacgcac aagtacggct acgccctcaa gggctccagc gtcctgctgt
     7021 accgcgacaa ggagctgcgc aagcaccagt acttcacgta ccccacctgg cccggcggcc
     7081 tgtacgtctc gccgggcttc gccggctcgc gcagcggcgg catcctcgcg tccacgtggg
     7141 ccgcgctggt ggccacgggg gagtcgggct acctcgcggc cgccgacgcc atcatgagga
     7201 cggcgaccac catcaaggag gggatccgcg acgggatccc cgagctcgaa gtcatcgggg
     7261 atcccctctt cctcatcgcc ttcaaggcgg cggacgacct cgacatctac ctcgtcaacg
     7321 actcgctcat cgcccagggc tggcggatga tcgccctgca gttgccgccg gcgctgcact
     7381 tctgcatcac ccgcccgaac gcgcgccccg gcctcgccga cgcgtacctc gaggcgctgc
     7441 acaccgccgt cgcgtacgcg cgcgacaaca agggcaagaa ggcgctgtcc ggcgccgtgt
     7501 acggcttcgg cggcacggcg ctcggtaccg agatgctcaa gttcgccatg gcccgcttcc
     7561 tcgacgccat gcacgacctg ccggacgagc cgggcgccgc ctgatgagcg agccctgggt
     7621 cctcgccgtc gacctcggca acggcggtcc caaggtcgcc gtcgtctcgc tcgaggggga
     7681 ggtgctgcgg gtcgccttcc gtcccgtgca cgcccgcatc ggcctcgacg gcgccgccac
     7741 ccaggacgcg gtcgagtggt gggccggcct gcgctcagcc gcgcgcgagg cgatctccgg
     7801 ggtgggcgcc gacggggacc ggctgcacgt cgtcgcgatc accggccagt acgggtcgtc
     7861 ggtgccggtg ggggccggcg gcgagccggt gggggaggtg ctgctctggg ccgacacacg
     7921 cgcgcgcgag ctggcgcgcg aggtcatcgg cggcccgctg aacatcggcg gcttcgcccc
     7981 gcacaaggtg ctcccgttcg tgcgcatcac cggcggcgcg ccgtctccca gcggcgccga
     8041 ccccacgggt cacgcgctgc tgttgcgcga gcgcctgcgt gaggtctacg agaagaccag
     8101 ggtcatcctc gagccggtgg actacctggg gctccgcttc accggtcgcg cggctgcgac
     8161 accggcgtcc atgctggcct cctggctcac cgacaaccgt atcggagctc cgttcggcta
     8221 cgtcgacgag ctcctggtgc gggcgcggcg ggagaagtcc aagctccccg agctcctgcc
     8281 caccggcgcg gtgctggggc cgctgcttcc ggacgtcgcc gaggagctcg gcgtcgccgc
     8341 gggcgtgccg gtcgtctgcg gcgtcccgga cctgcacgcc gccgtcatcg gctcgggggc
     8401 catcgcgccc tacgacaccc acgtggccat ctccacgacg gcgtggatca gctcccgggt
     8461 ccccttcaag cgcaccgaca tcctgcacca gatcgccacg gtccccggca tcgacgcggc
     8521 gcatccgatc gtcgtgaaca accaggagac gggaggcgcc gcgctccact ggctgcgcga
     8581 gcagatcgtg gcgcccaacg acggcctcat gggcggcggc agcgggatcg gggcagaggg
     8641 cgcggccgcc gaggccctgg cgccctcgtt cgacgacctc atacgtctgg cggcgcaggc
     8701 tcccgccggg tgcgagggcc tgctgttcac gccgtggctg aacggcgaac ggagccccgc
     8761 cgaggacaag gtcgtgcgcg ccggctggct caacctctcg ctgcgctcag accgcgcgat
     8821 gctggtgcgc tcggtcctcg agggggtggc ctacaacgcc cgctggctgt tcgacgccta
     8881 cgagaagttc ctgcggcgct ccgtccccaa ggtacgcatc ctcggcggag gcgcacagag
     8941 cgagctctgg tgccagatct acgccgacgt cttcggacgg cccgtcgagc aggtcgcgga
     9001 cccgcgcaac gcgcagctcc gcggcgtcgc cctgtgggcg cgcatctgcc tgggagagct
     9061 gacgctcgac gacgtccccg ggctcgttcc cgtgccggag agcttcgtgc ccacggatgc
     9121 cgccgccgtc tacgcccgcg ggtacgcgga gtaccgcaag ctgttcggcc gcctcaaggg
     9181 cctctatcac cgcctgaaca gcggctgagc gcccgacttc ctactcgaga tactcctgca
     9241 ggcactgcgc ctcgcggatc gccttgagct tggccagcgt ccgcgcctcg atctggcgga
     9301 tgcgctcgcg cgtgaccccg aacacctggc cgacctcttc cagcgtgcgg gggcggccgt
     9361 cgtcgaggcc gaagcgcagg cggagcacgg tgcgctcgcg gcgcgagagc aggccgagca
     9421 cggccatgat ctcctcgtgc cggacgatct cggcgacctc ctcgacgggc gccgccgact
     9481 tggtgtcctc gacgaagtcg ccgagctggg agtcgccctc gtcgccgacc tgctggtgca
     9541 ggctcgccgg ctcctggctg atcctctgga tctcgcggat cttggccacc gtcacgccca
     9601 tctcggcggc gatctcctcg gggaccggct cgcggccgat ctcctggatg agcagccgct
     9661 gcacgcgcac gtacgtctgg atgttctcga ccatgtgcac ggggacgcgg atggtgcgcg
     9721 actggtccgc cagggcccgg gtgatggcct ggcggatcca ccaggtggcg taggtgctga
     9781 acttgaagcc gcgccggtag tcgaacttct ccacggcgcg catcagcccg aggttgccct
     9841 cctggatgag gtcgaggagc ggcatgcccc tgccggcgtg ccgcttggcg atgctcacca
     9901 cgaggcgcag attggactcg atgagcttgc gacgcgcggc cgcgtcgccg cgctcgatcc
     9961 gtctggcgag ggcgacctcc tccacgccgg tgagcagcgg caccttgccc atctcgtgga
    10021 agtagaggcg cacgggatcg ctgctgacgg ccttgacgga gaggtcgagc ttggccccgg
    10081 cgtcgccgtc ctccgccgcc tcgccggcgg cgtcggtctc ggcgcgggcg tcgtcctcga
    10141 tgatctcgat gcccatctcg gcgaggacga tgagcagttc ctcgagctgg gtggccgtga
    10201 cctcgacgtc gcgcagcgcc gtggccacgt cgccgctgct caggcagccc agctcgtggc
    10261 cctcctcgat gaggcacgtg acctcggcgc agtcgatcag cgccacggag ggcgcggtgg
    10321 tctcgtctgc cggagccggt tcgggcacgc gctgtctcct gccgttgatc ctgcgcccag
    10381 gtgatcggga cggcgaatgc gcggaggagc cggggcgccg gcgtccgccg ctcgcggcgg
    10441 gcgacgcggg cgccgagtcg agcacgagag acgtcaggcg acgagcggag cgaggctgcc
    10501 agtgtagcag gccggtgccc ccggcgctcg ttcgcgaggc tgcgcgacgt gaggccgggc
    10561 gcgggcgcgc cggcgcgcac gatcgcccgc catctggtac actctcgcgc gacgggtcga
    10621 gttgggcccg cggaattgcg acgtgacaca ccgcatttcg acgcggcggt cacgtctttt
    10681 tttgcccgag tgagggcgaa gggacttgat cagcatggca acaggcaaag tgaagtggtt
    10741 caacgcggcc aaggggtacg ggttcatcga gcaggctgac ggcgacgatc tgttcgtcca
    10801 cttctctgag atccagagcg agggcttcaa gtccctcgac gagggtcagg ccgtctcgtt
    10861 cgaggcgggc accggcaaga acaacaagcc gcaggcgacg aacgtgcgcg tcatctcgta
    10921 gcactgcgtt tcgcacttcg cgggtcccga ggaccgtgcg gctccacgag ccgcacggtc
    10981 ctcgttcttc atggtgtgcc ggccgcgcct gaagccgcct cgtggatcgc ccgcgtcagg
    11041 gcgtcgtggc gcgcatcctt gatgaggtag ctgaccgctc cgccctcgag cgccgctttg
    11101 gccagcgcgc tgtcgccggc gcccgagagg atcaccacct gcacgcgcgg gtacgcgtcc
    11161 cggatccgcg ccgtggcctc cgcgccgtcc atgaccggca tgcgcaggtc catcagcgcg
    11221 acgtccggga ggacgcgggg caccagctcg agcgcctcca gcccggtgga cgcctccccg
    11281 acgacgtcga tcgcgggatc tctcgacagc agggccttca ggccctcccg gaccagctcg
    11341 tggtcgtcga cgatgaggac tctgactcgc ccctgcggca cccacacctc ccggcacccg
    11401 taccactgac ctccgccgga gatacctccg ccggtgattc cattctcttc cagtctgccc
    11461 ggaccagccg acgaatcagc gtcgacttcc tgctccggcg ggcgggcggg cggagcgagg
    11521 ccgcgagggc cggcgcaccc ggaccgggcg ggcgacgagg tgcggagacc ctcagcgccg
    11581 ggcggccgcc cgggcggggt agatgcgcaa cgcgtgtcgc accggcagtc ccggccgagg
    11641 cgcccggcgc gccttccgtg gcccggcgca ggcgcggtcc cggcgccgaa ggcgcggcgc
    11701 cggctgcccg gtcgcccccg gtcagcccag gacgtacggc aggtccgacg tggccgtcgg
    11761 gtagctccac gtcatggccg tgagatcggc ggcggtgagg ccgtgcctga cggccagggc
    11821 gaggacattg acgacctcgt cggcgttgac gccgagcagg tgcgcgccga ggaggcggcc
    11881 gctcgccgcg tccgtgacga ccgccatgcc ggcgtgcgtc tggccgacgc gccgctggct
    11941 gaaccactcg ctcgcgtcgg cgcgctcgac gtggacggcg tctccccggc cttgtgcctc
    12001 ggccgtgctc atgccgacgg cggcgagggg cgggtcgctg aagcacacgc tcgggacggc
    12061 gcggccgtcg tagacggcgt cctcgccgag gatgccggcc gcgaccacct tgccctcttt
    12121 ggaggcgacc ggcgtgagcg gcagtccctt gtcggcggcg tcgccggccg cccacacgtg
    12181 cgcgtcgtcc acgctccgca ggtgctcgtc gacgcgcacc ccgcgcgggc cggccgcgat
    12241 gcccgcggac tcgaggccga ggtcatccag gtccgcgacc cgcccggcac cgtgcaccac
    12301 gaggtcggcg gcgacctcgc cggtctcgtg cgcgaccacg agctcatcgc cgcgccgggt
    12361 cacctcgttg accggcgcct ccgtgagcac gtcgatgccg agcgagcggt accgctcgac
    12421 gagcgcgtcc gcgagctgct tgtcgaaacc cttcagcacg tgcgcggagc ggtgcaggat
    12481 cgtcactgcg gcgccggcca tgcgcgcgag ccaggcgaat tcgaacgtga tgtagccgcc
    12541 gccgatgaag acgacccgcg gcggcaggtc ccccagttcc aggaacccgg tgctcgtggt
    12601 caccagctcc tggcccggga tgcccagatc catgggccgg gcgccgctgg ccacgaccac
    12661 ctcggcggct tcgagtgtcc ggccgtccac ctgcacctcg tgcgcgctcc gcagtcgcgc
    12721 cgtgccgtgc agggtctcgg cgccggcgtc gcgcatccag ccctcgatcc tctcgggtaa
    12781 cggctccgtg aacgtgcgct tgcgcgcgat cagggccggc cagtcgatcg tgggcgggcc
    12841 cgtgagcccc cggccgtcca gcccgcgcgc gcgactcacg gcctccgccg ccgcgagcag
    12901 gaccttcttg gggtcgcagc cgcgctgccc gcaggtcccg ccgtaggcga ggcggtccac
    12961 gaccgccaca cgcaggccct cctgcgcgca gcgcggcgcc acgttctggg cggccgtgcc
    13021 gctgccgatg acgacgacgt cgtatccggg catcgctcac tccttcgctc gcctaggccg
    13081 tggtatgccc ggtatgcggg acgccggcac ccccgggccg gacaggcgtg agcgctcgcc
    13141 gttcaaaccc gttcatgagt gtttgggatt cattcacgat cggtcaggtg cggatgcgca
    13201 gcgcccttga cacgcgatca tctggtcgta ggatgcgatc atctttgaac gggagtgatt
    13261 gttgatgatt gatggcccgc aggcgtaagg ctgcaaaagg ggagcgggtg gcgacaccgg
    13321 cgccgcgaga gatgttgccc gcagagcgtc gcgcgcgcat cgtctcctcg ctcaaggacc
    13381 agcgcgccgt ccgcgtgccg gcgctgagcg aggacctcgg cgtctcggag atcaccatcc
    13441 gccgcgacct gctcctgctg gagcaggagg gcgtgctcga ccgtacctac ggcggcgccg
    13501 tggccaagaa gcagctggcc gcggagtcac gctgggaaga ggccgcggcg gtcaacgccg
    13561 cgcagaagga cgccatcgcc gctgccgccg cggagatgat cgagccgggg gacacggtct
    13621 tcttcggctc gggcaccacc gtcgtcggca tcctgcgcca cgtcgacccc gagctcgagg
    13681 cgcgcgtggt cacgcacagt ctcgccgtgg cggccgaggc tcgtggcctc agtctcgagg
    13741 tgctcttcct gggcggtctg taccggccac agctggacgc cgtcgagggt acgtggccgc
    13801 tggacatgat caatcaggtc tacgccgaca aggcgttcct cggcgccgac ggcctggacg
    13861 tgcgcgcggg gctcacctcg ccgagcatgg cggcggcgac cgtcgaactg gcgatgatcc
    13921 gccgcacgcg cggcgaggtg gtcgtgctgg ccgactccag caagatcggg ctggtcggcc
    13981 cgctggtcat ctgcccgctc gaccagatcg acgtggtgct ggtcgacgac ggcatcgatc
    14041 ccgcggtcgt caagcagatc cagcgcggcg ggccccagtg cgaagtggtc ccggcgtgaa
    14101 ggtccccgtg gagacgaagg aagggtggac gaggtggcat cggtcgagca tctgacgccg
    14161 gaggcggcga tcgcccgcat cccggactgg aacggcctcg acgtacaggt cgagaccctg
    14221 ggcggcggca tcaccaacca caactacgtc gtgaccgtgt ccggccgccc ggatctgccg
    14281 tggggcggca agtacgtcat gcgcatcccg gggcagggca cggaccgctt catcgaccgc
    14341 gagcgcgagc accgcaacgc cgttgcggcg gccgaggccg gcgtcacgcc gccgatcctg
    14401 cacatgctcg agcccgagat gtgcaccgtg gtccccttca tcgagggcga gacgatgcac
    14461 ccggaggacc tcgccggcca tcccgaccgg ctggagaagg tcgtcgacgt catgcgcgcc
    14521 taccacgaca aggccacctt cgcgaacgac atccacgtgt tcgacatgat ccgcgagtac
    14581 cttgacatgg cgcgcggcgt ggacgcgacc ttccccgacg acatggactg gatgctcggc
    14641 ctgggcgaca ggatcgagga ggcgatgaag cgcgaccagc ccgcgcccgt cgcctgtcac
    14701 tgcgatctgc tctccgagaa cttcatcctg gagccgggcg ggcgcatgtg ggtgatcgac
    14761 tgggagtacg gcggcatggc cgacccgtac ttcgacctcg gcgacttctg cgtggagcac
    14821 cccttgtctg acgacgagga acgcttcatc atcacgcgat actgcgggtc cttcagcgag
    14881 gagcggtacg cgaggatgat gctgcacaag atcgtcgccg acctctggtg gagcatctgg
    14941 gcctggatcc aggtgcggct ctcgaagatc gacttcgact tccaggacta cggcatgaac
    15001 cgggtggcga ggctgcgcgc caatgctgcg aggcccgagt tcgagtcgtg gctcgcaacg
    15061 gtgtaggtgg aaatggagca gcgcatgaac gatgacggaa cgcgcccggc cggtgagatg
    15121 ggcccggacg tcggtatggg caaggtcgac ccggagatgg ctcgcacgtt ccaggaggaa
    15181 gccgtccgcg tcgtcagggc cggcgaggag cagggcgtgc acctgcgtgt catcggcgcc
    15241 ctcgccttct ggcaccactg cccggagttc gggtacctgc aggagaagct gaaccgtgtg
    15301 tacacggaca tcgacttcgc cggctacggc aagcaggacc agcagatccg caagctgttc
    15361 gtctcgatcg gctacgacga ggacctcgag atcaacgcct tccacgccga gggcggccgc
    15421 ctgatcttca acaaccccac gaaccacatc cacgtggacg tgttcatgga caagctggac
    15481 ttctgccatc ccatcccctg gaagggcagg ctcgaggtcg actcgccgac gatcccgctg
    15541 gctgagctgc tgctcgagaa gatgcagatc gtcaagatca acgagaagga catcatcgac
    15601 acgatcatgc tgctgctcga gcacgagatc tgcggcgacg acgaggacca catcaactcg
    15661 cagcacgtcg ccaagctgtg cgccggcgag tggggcctgt ggcgcaccac caccatgaac
    15721 ctcgagaagg tgcgcgacta cctgccgcac ctcgacctcg acgaagcgca caagcagatc
    15781 gtgacgacgc gcgtcgccga gctcatgagc gccatggacg actaccccaa gggcacccgc
    15841 tggaagctgc gcgcgcgggt gggcgacaaa gtcaagtggt acaaagagat cgattagggg
    15901 gcggatgacc catgggactc ttcagcagaa agggcggctc gagcgacgat cgcgtgcgca
    15961 tcttctttgc caccgacgtg cacggctcgg agcgcaccta ccgcaagttc gtgaacggcg
    16021 ccaagttcta caaggtcgac catctcgtca tgggcggcga tatcgagggc aagttcctcg
    16081 tgccgatcgt cgccgagggc ggcggtcact accgggtcac gctgcaggag gacatctctc
    16141 atctggacgg cgacgacgag ctcaaggcgc tcaaggagcg catcgagacg ctcggcttct
    16201 accacgtcgt cgtgaccgag gaggagatcc gcgagatgca ggccgaccag agcctcgtcg
    16261 acgcggcctt caagcgcgag gcccacgcgc gcctcgagcg ctggatcgcg ctcgccgacg
    16321 agcgcctggc cggcaccgac accatgatgt acgtgaccgg gggcaacgac gacccgcagg
    16381 agctcatcga cacgctcgag gagaagcaga gcgaccacgt gatcaactgc gagggccgca
    16441 tcgtgatgct gagcgacacc ttccccatgg cgaactgcgg gtacagcaac cccacgccgt
    16501 ggaacacgcc gcgggagacc gacgaggaga cgctggagaa gtacctggag gagtcgctcc
    16561 aggggatcga cgacttcacc agcgcgatct tcaacttcca cgtcccgccg tacgactcca
    16621 cgctcgatga ttgtccgaag ctcgactggt cgacggaccc gccggcgcct gtcgtcgtgg
    16681 ccggcgtgcc gcagtcggcg ccggccggca gccacgccgt ccgcaacgtg gtcgagcgcc
    16741 atcagccgct gctcatgctc acggggcata tccacgagtc gcgaggcctg gtgaaggtca
    16801 agcgcaccac cgtggtgaat ccgggcagtg agtacggcga ggggatcctg agggggtgca
    16861 tcctgacatt ggcgcccggc aaggtcgtgg gttatcagat gacgtccggg tgaggagaag
    16921 gggcagcctc gccgaacgtg tctgtgaagt ctcgagagac tgacgaagag ggggagagat
    16981 ggctacaacc gcagcgaaac ccgaggtctt cagccggaag gcttcggggc tgtccagagt
    17041 gatgtcgccc tggtcggcgt tcatgtacaa cttcctgacc atgggcgtga tcttcccgtg
    17101 gacgttcgtg tgggcgcctg ccgcgttccc gggcgtctcc gtgtggaagg cgtgccttct
    17161 ggcgatcctg ctcgagctgc ccatcgcgct cgcctacgtg tggatggcga ccgcgatgcc
    17221 gcgctccggc ggcgactacg tcttccagag ccgcgtgctc gggggcgcta tcgggttccc
    17281 ggtcgtgatg agcggcttcg tcatctggat cctgcagtgg gtggcgctcg ccggctggct
    17341 gctcgccact ctcggtgtgg cgccgctctt catggggctc ggggtgtact acgagaacca
    17401 gggcctcatc gacgctgcgg tctgggcgca gggaagccag ggcgtggtca ccatcagcat
    17461 catcggcgcc gccgcgatga tgatcctgct cgtgaccggc ttcaagaact acgtacgcct
    17521 gcaatacgcg atgttcgccg ccaccggcat cctcgtcgtc atcctgctcg tgcagttcct
    17581 gcgcaccaca ccggcggagt tcgccgttgc catgaaccac ttctccaacg tcgtggacgg
    17641 ccgcacggac ttctaccggt ggctgcagaa ggacgtggcc gacgccgggg tgaacctgct
    17701 gccggccatc acgttcgggg cgatcctgct cgccacgccg atcgtgtgga ccagcctgca
    17761 gtgggccacg tacagcgtgc agcagggcgg ggagatcaag ggcgcccgtg tgttcaagaa
    17821 ccagatgttc atcatcatcg gctcgatgat cgccgtcggc gtcgtcctcg ccctcatcgc
    17881 ctgggccgag gagcgcgcgg tgggtaccgc gttcttcaac gccgtctcgc actcgtacta
    17941 ttacgtggtg agcgcttccg gcgacgggat aggcagcgtc ctgcccttcc cgggcatgtt
    18001 cgcgatcgtg atctcgccca acccgatcat cgtcatcctg gtcgcggtga gcttcatgct
    18061 cgcgtcgctg cagatcacct gcaactgcta catcggcatg acccgcatca tggtcggcat
    18121 gtcgctcgac cgcacgctgc cgacctggtt ctccaaggtc agcccgcggt tccgctcgcc
    18181 ggtcaacgcg cacctgatct acttcgcgct cggctgcgtc gcgatcctcg gctacaacta
    18241 ctggacgcag tggtactcca tgacgctcgg cgtgacgctc gcgtgcggct acgtgttcac
    18301 catcagctgc ctggcggccg cgctgctgcc gttccgcgcc aaggcgctct acgaggcctc
    18361 tccgggcgcc aagtacacgc tcggcgggtt cccgctggtc acgatcttcg gcatcatcgg
    18421 cttcgtgttc ggtctgtggg cgctgatcat gttcctgacc aactcggcct acggcctcac
    18481 gggcacgacg ccgtacatcg tcgtggccgg tatcttcgtc gtctgcctga tcggctactg
    18541 gatcggccgc gcctacaaca agggcaaggg catcgacgtc aactacgcct tcctcgaggt
    18601 gccgccggag tgagcctgag ccggtagtac gcgaggcgcg ttcttcggag ggcgccgggg
    18661 ctccgagccc cggcgccctc cgcgcgtcgg cgccgcgccc ggctcacgag cgccgcttcg
    18721 ccgccacggg cgcgcgtggg agaatggacg ggcaccgccg gccgggccgc cgaaggcacg
    18781 cccggctgga ccacccgtag cgccagagga gaccatgggg acccccgaga ccatcgccgt
    18841 cgagagcgcc cacatgcccc cgttcttcgc caagacgccc atctcgatcg cgcgcggcga
    18901 gggcgtgtgg gtgtgggacg aggagggccg gcggtacctg gacctcaccg ccggctgggg
    18961 cgtcacctcc atcgggcacg cccatccggt gatcgtgcgc gcgctggcgg agcagggcgc
    19021 gcgcatcatc cagaacccgg actccgggct gacctactcc ccggcgcggg cgcggctgct
    19081 cgagctgctc gccgggatcc tgccggccgg gctcacccgc gtcttcttca ccaacagcgg
    19141 ggccgaggcc aacgacgcgg ccgtcaagct ggcgcgcaag gccaccggcc gcaccgaggt
    19201 catcgccacc gagggcagct tccacgggcg cacgatcagc atggtctcgg ccaccggcca
    19261 ggccaggcac cgggacaagt tccggccgca gatgccgggc taccgcttcg taccctacgg
    19321 ccgggcggac gcggtcgcgg ccgcgctcgg cgcggacgtg gcggcgctca tcgtcgagcc
    19381 ggtgcagggc gagggcggcg tgcgcatccc cgcgccggac tacctgcgcg agctctcgcg
    19441 gctgtgccgc gccaacggca gcctgctggt cgtcgacgag gtgcagaccg ggttctgccg
    19501 caccgggccg ctgttcgcga ccgcggccgc cggcgcggag gcggacttcc tcaccatggc
    19561 caagggcatc gccggcggct tcccgctcgg cgccttcgcg atgacggacg ccgtcgccgg
    19621 gcggctcgag gcgggcgacc acggcggcac ctactgcggc aaccccctcg cctgcgccgt
    19681 ggcggaggcg gtcatccggc acctcatcga cgagggcgtg gcggcacgcg tcgaggagct
    19741 gggcggcgag gcgctcgcgc gcatgggctc ctggcgcgag cgcttccccg agaccgtcgc
    19801 cggcgcccgc ggcgccggcc tgctgctcat cgtcgagctc accggagagg aggcggccgc
    19861 cgccgtcgcc gccgagtgcc tgcggcgggg agcgttcgtg cggcagaccc agggcaacgg
    19921 gatccgcgtg ttcccggcgc tgaccatcac gcgcgcggag ctggaccagg ggctgaccgt
    19981 cctcgaggag tcgatcgccg ccgtcgccga cagggccggc tgacgccggg cgaggaggaa
    20041 cgacatggca caggtgagcg tgctcggact gggcgcgatg ggcagcacgc tcgcgcgggt
    20101 gctgctggag cgcggtcatc gcgtgaccgt gtggaaccgc agcgactcgg agcgggcggc
    20161 ggacgtggcc gcggccgggg cggcgcgagc cgccacgccg gcagcggcgg tgtccgccgc
    20221 cccgctcgtc ctcatgtgcg tcaccgacta cccggcggcc ggggacgtcc tcgcggcgcc
    20281 cggcgcgctc gccgggctcg ccgggcgcac cctggtgcag ctcaccaacg gcaacgagga
    20341 gcaggtgcgc gcgcagatgg cgcgggtgcg tgccgccggc gggcgcatgc tggccggcgg
    20401 catcctcggg tacccgcggc acatcggagg gcgggagacg gtgatcatgt acgcgggcga
    20461 cgccgaggcg ttcgaggagc acgccgccac gctggccggc ctcgccgggg cgcagcgctt
    20521 ctccggcgcc gaccccggcc cgccgaacgc ggcgtacgtc gcggcgttcg gcttctactt
    20581 cgcggcgctg gccgggtttc tcgaggccgc cgcgctcgcc gcgtcgcgtg gcgtcgcccc
    20641 ggcggagttc gccgcggcca tgccgggcat gacggcgctg ctgctcgacc acatcgacga
    20701 cgccgcacgg cgcatcgagg acgacgacta cgccggcgac caggccacgg tggacgtcca
    20761 cctggtcggg tcggagcgcc ggcggcggac gctcgcggac gccggcctgg cgtcgctgat
    20821 gaccggcgcc ttcgtcgagt actgccgccg ggcgcaggcg gcgggggagg gcggcgagga
    20881 cgtcgccgcc ctcttcaagc gcatgcgtgc tccgtcgccc tgagtacgga gagcgccccg
    20941 tcccgcgtcg ccccgccccc cacggcaaca gaaaagaagg ggggcgggac cccttgggcc
    21001 ccgccccccg acgatcgagc cgcggtgtgc acgccgcggc cgcctgaggt cgtggagtat
    21061 ctcagtcgcc ttccgtcttc gagccgtcgg tcacgatgcc gtcggtgctc cgcggcgtga
    21121 agtccgggat gtgcgggatg tagcgcgtct cggggccggg catcgcggtc gggttgtagc
    21181 cgctggtcgt catgtcgtgc ctccgtttca cctgctcggc tgcgccgagc tcgtacagtc
    21241 gatccggggc gacaaaaaag cgcccccggt cgcttgggac cggagagcgc cattgctctc
    21301 gtctgaagcc ggcacgccgt tgtgccgagc ctcagcgtag cacataccca ttgtgcgcag
    21361 aagcgacgca acggctcgcg cctgcccggg aggcctctac tccatcgcct cgtgcgccgt
    21421 gacgaccgcc tcggcgacct cgcgcagtga cttgcgcttg tccatcgcca gcttctgcag
    21481 gcggtggaag gcctccggct cgctgagccc cttggccatc agcagccctt tggccctgtc
    21541 gagcagcttg cgcgtctcga ggcgctcctc gaggtcgccg acctgctgcg cgagcaggct
    21601 catctcggcg tggcggctca cggcgacctg catcgccggc acgatgtcgt tgcgggagaa
    21661 gggcttgacc acgtagccca tggcgccggc gcgggcggcc tcctcgacca ggccggcctg
    21721 agagaaggcc gtgaccatga ccaccggcgc gatggactcg gcgctgatgg cggacgccgc
    21781 ctcgatgccg tcgacgttgg gcatcttgac gtccatgaac acgatgtcgg ggcgcgtggc
    21841 acgggccagc gtgagcgcct ccgcaccgtc gcgcgcctcg ccgacgacct cgtggccctc
    21901 ctcctcgagc atctcgcgca ggtccatgcg gatcagcgcc tcgtcttcgg cgatcagcac
    21961 gcgcacgcgc cgcacctcct cctcgtcgtc acagactgtc cccgcctctg cccagcggca
    22021 cacggatggc gatgcccgcg ccctcgccgg ggctcagcga gagcgtcccg cccaggtcct
    22081 cctccaccag cgagcgcacg atggagagac cgaggccctt ggaggtggct gggtcgaagt
    22141 ctgcgggcag gccccggccg tcgtcgcgga cgatcagcat gaggccttcg gcgtcgcggc
    22201 tgaggtccac cgagaccgtg ccacggtcga ccggcccgaa cgcgtgctcc agcgagttgt
    22261 gcaccagctc cgtgagcacc aaggccagag aagtcgccgg gccggcgtcg acggggccgg
    22321 tgaccccgga cacgcgcacg gcgaagcgcg ggtcgtcgcc gaccacgctg ctgcgcacca
    22381 ggccgaccac gcgtcgcgcc acctcggcga agtcgacgca ctcctccgcc gagtgcgcca
    22441 gcatgtcgtg cacggtggcc atcgcggcgg cgcgcccgac cgcctccgcc agggcgcgcc
    22501 gcacctcgtc gctcccgctg cgccgggcct gcaggcgcag cagcgaggcg atggtctgca
    22561 ggttgttctt gacgcggtgg tggacctcac ggatggtcgc ctccttgacg ccgagctcgc
    22621 gctcgcgccg gcgcgcctcc gtgaggtcct cgaccagcac cagcgctcct g
//
LOCUS       DASUMX010000166         3094 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_345241, whole genome shotgun sequence.
ACCESSION   DASUMX010000166 DASUMX010000000
VERSION     DASUMX010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3094
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345241"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="VFZ86_08365"
     CDS             complement(<1..198)
                     /locus_tag="VFZ86_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014515963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HEX5642336.1"
                     /translation="MAGPLRIVVVGGGMAGLGAARVLEAARAQDPSVDWHLYEQDARL
                     GGKVHTVRRDGFVVEGGPDSAI"
     gene            complement(198..1157)
                     /gene="hemH"
                     /locus_tag="VFZ86_08370"
     CDS             complement(198..1157)
                     /gene="hemH"
                     /locus_tag="VFZ86_08370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEX5642337.1"
                     /translation="MTESRVDVALLAYGGPESPEQIVPFLERLMGRAPNPGTVAAVKE
                     RYALIGGASPLPAITARQAVALQAKLVQELAFPIRVRHGFLYTEPGVVDCVRSLDAHE
                     VVALPMSPFSSRLTSGRYLAQLAAAEDPTTGGHGRDIPLLEGWFASQGFVRTISRRVA
                     EALDGCDVSEWAVLFTAHSVPAETIDEGDPYVDQLQQTIAQLIPQIVPGDWRFGFQSK
                     GRGGGEWMEPEADDAVRVLAEDGWKKILVVPVGFVSDNVETLYDLDIVLRAQIEDLGI
                     EYRRSHAPNDSPFFIEALAGVVTDYLAHRPVALQRPDGPHSHG"
     gene            complement(1177..2202)
                     /gene="hemE"
                     /locus_tag="VFZ86_08375"
     CDS             complement(1177..2202)
                     /gene="hemE"
                     /locus_tag="VFZ86_08375"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEX5642338.1"
                     /translation="MRLLDACARKPVDATPVWLMRQAGRYLPEYRAIREKMSFVEMCR
                     RPDIAAEVTLQPLRRFELDAAIVFADILLPLEPMGIGFHFSKDDGPVIERPIRGARDL
                     EGVHSIDAASDLQYVMDTIKLVRHELDPSIPLIGFSGAPFTLASYIVEGGHSRNYAHV
                     KKLMYEDPVTFGRLMSVISEVVVDYLKAQIAAGAQVVQLFDSWVGWLGPYDYELLVLP
                     HVRFVVDQVKGLGAPVIYFGNGASAMLDLVGKAGSDVYGVDWRIELDRAWDQLGDVAV
                     QGNLDPITLLGPPKAIEERVADVLRRAGGRPGHIFNLGHGLIPQVPPDNVKYLVDAVH
                     RLSAASL"
     gene            complement(2360..2599)
                     /locus_tag="VFZ86_08380"
     CDS             complement(2360..2599)
                     /locus_tag="VFZ86_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642339.1"
                     /translation="MGQVHPMPNQSEDHAATVADKPPATVRSIDSAHCGLCGAKLSAR
                     ALRSRVVSPQCCDSTITVCRTCRKAALSEGYRPAE"
     gene            2840..>3094
                     /locus_tag="VFZ86_08385"
     CDS             2840..>3094
                     /locus_tag="VFZ86_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642340.1"
                     /translation="MKRLLVAAALCAAILLLAAPVALAASAPLHAAGHSGASARAPFA
                     GDRMDRAPLKFEPLARRILTGSGAISLNVYPFNGPAEVGAE"
BASE COUNT          461 a         1050 c         1138 g          445 t
ORIGIN      
        1 gatcgccgag tcagggccgc cctcgacgac gaacccatcg cggcgcacgg tgtgcacctt
       61 gccgccgagg cgggcgtcct gctcgtagag gtgccagtcg acggagggat cctgcgcgcg
      121 cgccgcctcg aggacgcgcg ccgcccccag cccggccatg ccgccgccga cgacgacgat
      181 gcggagcggg ccggccatca cccgtgggag tgcggcccgt ccggccgctg cagggcgacg
      241 ggccggtgcg ccaggtagtc ggtgaccacg ccggccagcg cctcgatgaa gaacggcgag
      301 tcgttggggg cgtgtgagcg gcggtactcg atgccgaggt cctcgatctg cgcgcgcagg
      361 acgatgtcga ggtcgtagag cgtctcgacg ttgtcgctca cgaacccgac cgggacgacg
      421 aggatcttct tccagccatc ttccgccagg acgcgcaccg cgtcgtcggc ctcgggctcc
      481 atccactcgc cgccgccgcg gcccttgctc tggaacccga agcgccagtc accaggcacg
      541 atctgaggga tcagctgggc gatggtctgc tgcagttggt ccacgtaggg atcgccctca
      601 tcgatggtct cggccgggac gctgtgcgcc gtgaacagga cggcccactc gctcacgtcg
      661 cagccgtcga gcgcctcggc gacacggcgg ctgatggtcc gcacgaagcc ctggctcgcg
      721 aaccagccct ccagcagggg gatgtcgcga ccgtggccgc cggtggtggg atcctcggcg
      781 gcggccagct gcgcgaggta cctgccgctg gtgagccgcg aggagaaggg actcatcggc
      841 agggcgacga cctcgtgcgc gtcgagcgag cggacgcagt cgacgacgcc cggttcggtg
      901 tagaggaaac cgtggcgcac gcggatgggg aaggcgagct cctgcaccag cttcgcctgg
      961 agagcgaccg cctggcgcgc ggtgatggcg gggagcggcg aggcgccgcc gatgagcgcg
     1021 tagcgctcct tgacggcggc gaccgtcccc gggttcggcg cgcgccccat cagccgctcg
     1081 aggaacggca cgatctgctc cgggctctcg gggcccccgt acgcgagcag ggcgacgtcg
     1141 acgcgactct cggtcatgcc gtcatcatcc ggctgctcaa agcgacgcgg ccgaaagccg
     1201 gtgcacggcg tcgaccaggt acttgacgtt gtccggcggt acctgcggga tcagcccgtg
     1261 accgaggttg aagatgtgcc ccggccggcc gccggcgcgc cgcagcacgt cggcgacgcg
     1321 ctcctcgatc gccttgggcg gcccgaggag cgtgatgggg tccaggttgc cctgcacggc
     1381 cacgtcgccg agctggtccc aggcgcggtc gagctcgatg cgccagtcga cgccgtagac
     1441 gtcgctgccg gccttgccga cgaggtcgag catggccgag gcgccgttgc cgaagtagat
     1501 gaccggcgcc cccaggccct tgacctggtc gacgacgaag cgcacgtgcg gcagcaccag
     1561 gagctcgtag tcgtaggggc cgagccagcc gacccacgag tcgaagagct gcaccacctg
     1621 ggcgccggcg gcgatctgcg ccttcagata gtcgacgacg acctcggaga tgacgctcat
     1681 gaggcgcccg aacgtgaccg ggtcctcgta catgagcttc ttgacgtgcg cgtagttgcg
     1741 cgagtggccg ccctcgacga tgtagctcgc gagggtgaac ggcgccccgg agaagccgat
     1801 gagagggatg gacgggtcga gctcgtggcg caccagcttg atggtgtcca tgacgtactg
     1861 caggtccgac gcggcgtcga tgctgtggac gccctcgagg tcgcgggcgc cgcggatggg
     1921 ccgctcgatg accgggccgt cgtccttgct gaagtggaag ccgatgccca tgggctccag
     1981 cggcagcagg atgtcggcga agacgatcgc cgcgtccagc tcgaagcggc ggagcggctg
     2041 cagtgtgacc tccgccgcga tgtccggccg ccggcacatc tcgacgaagc tcatcttctc
     2101 gcggatggcg cggtactcgg gcaggtagcg gccggcctga cgcatgagcc agacgggcgt
     2161 ggcgtcgacg ggtttgcggg cgcaggcatc gaggaggcgc atgggcatca cctggtgagg
     2221 tggagcggga tggcagggac ggtgattgta acaaacggga gcccatgggc tcccgtcgag
     2281 gatgagatgg cgggccggcg gccgctcatt ggtcgccgta gacaaccagc caactggcgg
     2341 tgcaccgcag gtccaccggc tactcggccg gcctgtaccc ttcgctcaag gccgccttgc
     2401 ggcacgtacg gcacacggtg atcgtcgaat cgcagcactg tggcgagacg acgcgggagc
     2461 ggagcgccct ggcgctgagc ttggcgccgc acagaccgca gtgggcgctg tcgatggagc
     2521 ggaccgtggc agggggcttg tccgccacgg tggcggcatg gtcttccgac tggttcggca
     2581 tcgggtggac ctggcccatg gtgtcctccc tccagctgtg ccacggcctc cggggcgggg
     2641 gtccggcaag cacttgtggc gtattcactt cctccccgga atcggccggc cggtaacccg
     2701 gcttgaggcc cagaggactg ccggcgttgc cacacgcagc gacacctgga cgacccttcc
     2761 atggccgtgg tccgcagctc cgggtaggat ggctgcaggg gaagcgggcc tcccggcccg
     2821 caacgtggag ggagatcgca tgaaacgtct gctcgtcgcg gctgcactgt gcgccgccat
     2881 cctgctcctc gcggcgccgg tcgcgctggc cgcgagcgcg ccgctgcacg cagccggaca
     2941 cagcggcgcc tccgcacgcg cgccgttcgc cggtgaccgc atggaccgtg cgccgctcaa
     3001 gttcgagccg ctcgcccgtc gcatcctcac cggcagcggc gccatcagcc tcaacgtgta
     3061 ccccttcaac ggacccgccg aggtcggcgc ggag
//
LOCUS       DASUMX010000167         6135 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1928152, whole genome shotgun sequence.
ACCESSION   DASUMX010000167 DASUMX010000000
VERSION     DASUMX010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 6135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6135
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1928152"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="VFZ86_08390"
     CDS             <1..278
                     /locus_tag="VFZ86_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEX5642341.1"
                     /translation="GWNDLEWGPAGAGMARGLPAPATVYFVHSYAAVPADPSVVAATT
                     DYGGPVVAAVARDNVWAVQFHPEKSSRVGLALLAGFVATIRQEAGTP"
     gene            275..1000
                     /gene="hisA"
                     /locus_tag="VFZ86_08395"
     CDS             275..1000
                     /gene="hisA"
                     /locus_tag="VFZ86_08395"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEX5642342.1"
                     /translation="MIVFPAVDIQDGKAVRLRRGDFDDVTVFSDDPVDLARYWQDQGA
                     EALHVIDLDAARTGELTNFPIVEQIVKALDIPVQYGGGIRTPKSLAFMAGIGVHWVVM
                     GTAAVTALDLLDDAVNWLGDRLVVGLDCTDGMCATHGWQQRSQMSAIRMVKVLAEHGV
                     QRVVYTDTARDGMLGGPNFPGLKDLAEVSPLEIILSGGVALLDDLRRLKRLDEPKVVG
                     VIVGRALYEKSFSLPQALAVFAD"
     gene            1112..1936
                     /gene="hisF"
                     /locus_tag="VFZ86_08400"
     CDS             1112..1936
                     /gene="hisF"
                     /locus_tag="VFZ86_08400"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEX5642343.1"
                     /translation="MHRKRIIPCLDVRDGRVVKGVNFVDIRDAGDPIELASRYDAEGA
                     DELIFLDITASHERRDIIIDLAARCAEQVFIPFTIGGGLSSVEDVRAVLAAGADKVTM
                     NTAAVQDPGLVRRCAERFGAQCIVVAIDARRVPGSGEEGRPSRWEVYLHGGRTPTGID
                     AIAWAARAAEAGAGEFMLTSMDRDGTLDGYDLELTRAVAREVGVPVIASGGVGTLEHL
                     AAGLIEGEADAVLAASIFHYGTYSIAEAKEYLAARGVPVRPVAAAAGAAPDGAGSR"
     gene            1933..2634
                     /locus_tag="VFZ86_08405"
     CDS             1933..2634
                     /locus_tag="VFZ86_08405"
                     /inference="COORDINATES: protein motif:HMM:NF013468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HEX5642344.1"
                     /translation="MSGARRIDLARPDGAGGPRLAELLAAARLVCFPTDTVYGVGGVV
                     TPAVRAAVIAAKGREAGKPLQLVYPSLELLEASLALGPRLLGAARRLLPGPFTLLLPY
                     PPGLAFPPAGEVRRRAQGVGGERTETVATLGVRVPAWPAAARVLGELPFPLLASSANP
                     SGGQAPRSVDEVDGGLLAACDLVLDAGPVSGAASAIVDLSRYEAGGEWRILRRGAIDE
                     RGVAALLAGDGEESR"
     gene            2634..3527
                     /locus_tag="VFZ86_08410"
     CDS             2634..3527
                     /locus_tag="VFZ86_08410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEX5642345.1"
                     /translation="MRTLRFYQVDVFTDRPFAGNPLAVFPEAEGLTAVQMQAIASEMN
                     LSETTFVTPPEADGDARVRIFTPAVELPFAGHPSVGTACEIVRLRLVDVYEPITRVVL
                     ELGVGPTPVDVSVRGGVPVAATVHQRRPVFHAPAPRGSVAAVLGLDLEDLHPEFDPVP
                     VDTGLCYTIVPLVSQRALAGVSIDLELVADFERRHAEIYPCAFTGQDDPWLEARGMFP
                     LVGITEDPATGSAAGPLAAYMARAGVLPIDEERVVLQGAQIGRPSFLTVAVSGAPDAI
                     TDVRVGGPVQPVLTGELSLPE"
     gene            3664..4107
                     /gene="rpiB"
                     /locus_tag="VFZ86_08415"
     CDS             3664..4107
                     /gene="rpiB"
                     /locus_tag="VFZ86_08415"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HEX5642346.1"
                     /translation="MKIAAGADHGGFALKQAVVRRLRSLGHEVVDVGTDSEEVSVDYP
                     VYGHKVAEMVASGEAERGVLVCGTGLGICMSANRHRGVRAADCFTPHLAEMARRHNDS
                     NVLCMGGRILSEDEAWAITEVWLETPFEGGRHERRVAEIDGEEPA"
     gene            4140..5372
                     /gene="glyA"
                     /locus_tag="VFZ86_08420"
     CDS             4140..5372
                     /gene="glyA"
                     /locus_tag="VFZ86_08420"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEX5642347.1"
                     /translation="MYHLDPELNELLRCELIRQQETLELIASENFASRAVLEATGSVL
                     TNKYAEGTPGARYYGGCEVVDQVEQLAIDRCKEVFGAEHANVQPHSGSSANLAALFAL
                     LDAGDTMMAMALAHGGHLTHGMGINYSGRTYNVVPYHVRPDTEVIDFDEVRALALEHK
                     PKLIICGYSSYPRIVDFAAFREIADECGALLMADIAHVAGLIAAGVHPNPVPYCEVVT
                     STTHKTLTGPRAGIIMCRAEYADAIDRAVFPGMQGGPLMHVIAAKAVCLRIAQSDEFH
                     KLQRQIVKNCAVLAETLAEGGLRLVSGGTDVHLALVDLRPAGLTGLECQELLERVNIT
                     ANRNVVPFEDASFNVASGLRVGSPAVSTRGFMETEMRQTGELMLRAIAARDNDAALAE
                     IRHEVVELLDRFPLYEFL"
     gene            5372..5857
                     /locus_tag="VFZ86_08425"
     CDS             5372..5857
                     /locus_tag="VFZ86_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015721294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HEX5642348.1"
                     /translation="MGRAEGPGWHRPTWDEYFLQLARQAATRSTCLRRQVGAVLVRDK
                     RILATGYNGAPRGVAHCLDIGCLREQLGIPSGERHELCRAIHAEQNAVIQAAIHGVAI
                     EGATLYCTHHPCILCAKILINCGVHEIHYLEGYPDELSREMLEEVGVVLLKEELPSTG
                     C"
     gene            5935..>6135
                     /locus_tag="VFZ86_08430"
     CDS             5935..>6135
                     /locus_tag="VFZ86_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HEX5642349.1"
                     /translation="MGRLAHRTGAVDTPTDDRRMHQLATPLLGGLALYIGWMVPVMLL
                     VEVDRPVWGIIGGATIVVAVGLF"
BASE COUNT          821 a         2271 c         2175 g          868 t
ORIGIN      
        1 tcggctggaa cgacctggag tgggggccgg ccggcgcggg catggcccgc gggctgccgg
       61 ccccggccac cgtgtacttc gtgcactcgt acgcggccgt cccggccgac ccgtcggtcg
      121 tggccgcgac gaccgactac ggcgggccgg tggtggccgc agtggcgcgc gacaacgtct
      181 gggcggtaca gttccatccc gagaagagtt cgcgcgtcgg gctcgcgctg ctggccgggt
      241 tcgtcgccac catccgtcag gaggccggca ccccatgatc gtcttccccg ccgtcgacat
      301 ccaggacggc aaggcggtgc gcctgcgccg cggcgacttc gacgacgtca ccgtcttcag
      361 cgacgacccg gtggacctgg cccgctactg gcaggaccag ggagccgagg cgctgcacgt
      421 catcgacctc gacgccgccc gtacgggtga gctcaccaac ttcccgatcg tggagcagat
      481 cgtgaaggcg ctcgacatcc ccgtgcagta cggcggcggc atccgcaccc ccaagtcgct
      541 cgcgttcatg gccggcatcg gcgtgcactg ggtcgtcatg ggcactgccg cggtcaccgc
      601 cctcgacctg ctcgacgacg ccgtgaactg gctcggcgac cgtctggtcg tcggcctcga
      661 ctgcaccgac ggcatgtgcg ccacgcacgg ctggcagcag cgctcgcaga tgagcgccat
      721 ccgcatggtc aaggtcctcg ccgagcacgg cgtccagcgc gtggtctaca ccgacaccgc
      781 gcgcgacggc atgctcggcg gccccaactt cccggggctc aaggaccttg ccgaggtgtc
      841 gcccctcgag atcatcctct cgggcggcgt cgcgctgctg gacgacctgc gccgcctcaa
      901 gcggctcgac gagcccaagg tcgtgggcgt catcgtcgga cgggcgctct acgagaagtc
      961 gttctcgctg ccgcaggcgc tggccgtctt cgccgattga ccgccgcagg tggatcaacg
     1021 agtggggcgc cggtggtaca ccgctcaatg accggcgcag ctggatcgac gaggggtgcc
     1081 ccgtgaggca caccgctctc tgacggcgcc cgtgcaccgc aagcgcatca tcccgtgcct
     1141 cgacgtccgt gacgggcgcg tggtcaaagg cgtcaacttc gtggacatcc gcgacgccgg
     1201 cgaccccatc gagctcgcgt cccgctacga cgccgaaggc gccgacgagc tcatcttcct
     1261 cgacatcacc gcgtcgcacg agcgccgcga catcatcatc gacctcgccg cgcgctgcgc
     1321 cgagcaggtg ttcatcccgt tcaccatcgg cggcggtctc tcgagtgtcg aggacgtccg
     1381 cgccgtcctc gccgccggcg ccgacaaggt cacgatgaac acggccgcgg tgcaggatcc
     1441 ggggctcgtg cgtcgctgcg cggagcggtt cggcgcgcag tgcatcgtcg tcgccatcga
     1501 cgcgcgcagg gtccccggaa gcggggagga ggggcgcccg tcgcgctggg aggtctacct
     1561 gcacggcggg cgcacgccca ccggtatcga cgcgatcgcg tgggcggcgc gcgcggccga
     1621 ggccggcgcc ggcgagttca tgctcacgag catggaccgc gacggcacgc tcgacggcta
     1681 cgacctggag ctcacgcgcg ccgtggcgcg cgaggtgggg gtgccggtga tcgcctccgg
     1741 cggcgtcggc acgctcgagc acctcgccgc gggtctcatc gagggcgagg ccgacgcggt
     1801 gctggcggcg agcatcttcc actacgggac ctacagcatc gccgaggcca aggagtacct
     1861 cgccgcgcgg ggcgtgccgg tgcggccggt ggccgccgcc gcgggcgccg cgccggacgg
     1921 cgccggctcc cggtgagcgg cgcgcgccgc atcgacctcg cgcggccgga cggcgcgggc
     1981 gggccgcggc tcgcggagct gctggccgcg gcgcgcctcg tctgctttcc cacggacacc
     2041 gtgtacgggg tcggcggcgt cgtgacgccg gccgtgcgcg cggcggtgat cgcggccaag
     2101 ggccgcgagg ccggcaagcc gctgcagctc gtctacccct cgctggagct gctcgaggcc
     2161 tccctggcgc tggggccccg gctgctcggt gcggcgcgcc ggctcctgcc ggggccgttc
     2221 accctcctgc tcccgtatcc gccggggctg gcctttcccc cggcgggcga ggtgcggcgc
     2281 cgcgcgcagg gcgtcggggg cgagaggacg gagacggtcg cgacgctggg cgtgcgcgtg
     2341 cccgcgtggc ccgcggccgc gcgggtcctc ggcgagctgc cgttcccgct gctcgcctcg
     2401 agcgccaacc cgagtggcgg gcaggcgccc cggtccgtgg acgaggtgga cggcggcctg
     2461 ctggccgcct gcgacctcgt cctcgacgcc gggccggtga gcggcgcggc gtcggccatc
     2521 gtcgacctca gccgctacga ggccggcggc gagtggcgta tcctgcgccg tggagccatc
     2581 gacgaacgag gggtcgccgc gctgcttgcc ggcgacgggg aggagtcacg ctgatgagga
     2641 cgctgcgctt ctaccaggtg gacgtgttca ccgaccgtcc gttcgccggc aacccgctgg
     2701 ccgtgttccc ggaggcggaa ggcctcaccg ccgtccagat gcaggcgatc gccagcgaga
     2761 tgaacctctc ggagacgacg ttcgtgacgc cgcccgaggc ggacggcgac gccagggtgc
     2821 gcatcttcac acccgccgtc gagctcccgt tcgcgggaca ccccagcgtg ggcaccgcct
     2881 gcgagatcgt ccgcctgcgc ctcgtcgacg tctacgagcc catcacgcgc gtcgtcctcg
     2941 agctcggcgt cgggccgacc cccgtcgacg tctccgtgcg cggcggcgtg cccgtcgcgg
     3001 ccaccgtgca ccagcggcgg ccggtcttcc acgctccggc gccgcgcggc tccgtcgccg
     3061 ccgtcctcgg cctcgacctc gaggacctgc accccgagtt cgaccccgtg ccggtcgaca
     3121 ccggcctctg ctacaccatc gtcccgctcg tgtcgcagcg cgccctggcc ggcgtctcca
     3181 tcgacctgga gctcgtcgcc gacttcgagc gccggcacgc cgagatctac ccgtgcgcgt
     3241 tcaccggcca ggacgacccc tggctcgagg cgcgcggcat gttccccctg gtcggcatca
     3301 ccgaggaccc ggcgaccggc agcgccgccg ggccgctcgc cgcgtacatg gcgcgcgcgg
     3361 gcgtgctgcc catcgacgag gagcgcgtcg tgctccaggg cgcccagatc gggcggccga
     3421 gcttcctcac ggtggccgtc tccggggcgc cggacgcgat cacggacgtg cgcgtcggcg
     3481 gcccggtgca gcccgtgctg acgggcgagc tctccctgcc cgagtgatga gggccgcagc
     3541 atgactgata caatcgacgc cctgtagccg aggccgcggg cgggggcggc gggcgcgctg
     3601 cgaggccgcc ttccgtccgt tccgcgtgcg ctcgtcggcc gtcccgacca gaaggagctc
     3661 gtcatgaaga tcgccgccgg cgccgaccac ggtgggttcg cgctcaaaca ggccgtcgtg
     3721 cgccgcctgc gctctctcgg gcacgaggtc gtcgacgtgg gcaccgactc cgaggaagtc
     3781 tcggtggact acccggtcta cggccacaag gtcgcggaga tggtggcgtc gggcgaggcc
     3841 gagcgcggcg tgctcgtgtg cggcaccggc ctcggcatct gcatgtctgc caaccgccac
     3901 aggggcgtgc gcgccgccga ctgcttcacc ccgcacctcg ccgagatggc gcggcggcac
     3961 aacgacagca acgtcctctg catgggcggc cgtatcctca gcgaggacga ggcctgggcg
     4021 atcaccgagg tctggctcga gacgcccttc gagggcgggc ggcacgagcg ccgggtcgcc
     4081 gagatcgacg gagaggagcc tgcttgagcc ccgtcggccg cgagtggcgc cgggagcccc
     4141 tgtaccacct cgacccggaa ctgaacgagc tcctgcgctg cgagctcatc cgccagcagg
     4201 agacgctcga gctcatcgcc agcgagaact tcgccagccg cgccgtgctc gaggcgacgg
     4261 ggtcggtgct caccaacaag tacgccgagg gcacgccggg agcgcgctac tacggcggtt
     4321 gcgaggtcgt cgaccaggtc gagcagctcg ccatcgaccg ttgcaaggag gtcttcggcg
     4381 ccgagcacgc caacgtgcag ccgcactcgg gctcgtcggc caacctcgca gccctgttcg
     4441 cgctgctgga cgcgggcgac acgatgatgg ccatggcgct cgctcacggg gggcacctca
     4501 cgcacgggat ggggatcaac tactcgggcc gcacctacaa cgtggtgccg taccacgtgc
     4561 gccccgacac cgaggtcatc gacttcgacg aggtgcgcgc cctggcgctg gagcacaagc
     4621 cgaagctcat catctgcggc tacagctcgt atccgcggat cgtcgacttc gccgccttcc
     4681 gcgagatcgc cgacgagtgt ggggcgctgc tcatggccga catcgcccac gtcgccgggc
     4741 tcatcgccgc cggcgtgcac cccaacccgg tgccctactg cgaggtcgtc accagcacca
     4801 cgcacaagac cctcaccggg ccgcgcgccg gcatcatcat gtgtcgcgcc gagtatgcgg
     4861 acgccatcga ccgcgccgtg ttcccgggca tgcagggcgg cccgctcatg cacgtgatcg
     4921 ccgccaaggc cgtgtgcctg cgcatcgcgc agagcgacga gttccacaag ctgcagcgac
     4981 agatcgtcaa gaactgcgcc gtgctcgccg agacgctggc cgagggcggc ctgcgcctcg
     5041 tgtccggcgg caccgacgtg cacctggcgc tcgtcgacct gcggcccgcg ggcctgaccg
     5101 gcctcgagtg ccaggaactg ctggagcgcg tgaacatcac ggcgaaccgc aacgtggtgc
     5161 cgttcgagga cgcctcgttc aacgtggcca gcggcctgcg cgtcggctcg ccggcggtga
     5221 gcacgcgggg gttcatggag accgagatgc gccagaccgg cgagctcatg ctgcgcgcca
     5281 tcgccgcccg cgacaacgac gcggcgctcg ccgagatccg tcacgaggtc gtcgagctcc
     5341 tcgaccggtt cccgctgtac gagttcctgt gatgggacgt gcagaagggc cgggctggca
     5401 caggccgacc tgggacgagt acttcctcca gctcgcccgc caggccgcga cccgctccac
     5461 ctgcctgcgc cgccaggtcg gcgccgtgct ggtgcgcgac aagcgcatcc tggcgaccgg
     5521 gtacaacggc gcgccgcgcg gcgtcgccca ctgtctcgac atcggctgtc tgcgcgagca
     5581 gctcggtatc ccgtcgggcg agcgccacga gctctgccgg gccatccacg ccgagcagaa
     5641 cgccgtcatc caggcggcca tccacggcgt ggccatcgag ggggccacgc tctactgcac
     5701 ccatcacccg tgcatcctgt gcgccaagat cctcatcaac tgcggtgtgc acgagatcca
     5761 ctacctcgag ggctatccgg acgagctttc gcgggagatg ctggaagagg tcggggtcgt
     5821 gctgctcaag gaggaactgc catcgacggg ctgctgagcc tgtccagctc gccgcggaac
     5881 gcgatcctgg gcttcctgtt cgcggcgctc atcgtctacg cgctcacgcc gctgatgggc
     5941 cgcctggcgc accgcaccgg cgccgtcgac acgcccacgg atgaccggcg catgcaccag
     6001 ctggcgaccc ctctcctcgg cggcctcgcc ctgtacatcg gctggatggt ccccgtgatg
     6061 ctgctggtgg aggtggaccg gccggtgtgg ggcatcatcg gcggcgccac catcgtcgtc
     6121 gccgtcgggc tgttc
//
LOCUS       DASUMX010000168         5183 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1928322, whole genome shotgun sequence.
ACCESSION   DASUMX010000168 DASUMX010000000
VERSION     DASUMX010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 5183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5183
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1928322"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /gene="tsf"
                     /locus_tag="VFZ86_08435"
     CDS             <1..219
                     /gene="tsf"
                     /locus_tag="VFZ86_08435"
                     /inference="COORDINATES: protein motif:HMM:NF013085.4"
                     /note="EF-Ts; functions during elongation stage of protein
                     translation; forms a dimer; associates with EF-Tu-GDP
                     complex and promotes exchange of GDP to GTP resulting in
                     regeneration of the active form of EF-Tu; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEX5642350.1"
                     /translation="QIKGKPENVVEKIITGKLEKFFGETCLMEQAFVKDPDRTIEQVR
                     TDLVGVIGENVEVRRFVRFQLGETTVAG"
     gene            225..962
                     /gene="pyrH"
                     /locus_tag="VFZ86_08440"
     CDS             225..962
                     /gene="pyrH"
                     /locus_tag="VFZ86_08440"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEX5642351.1"
                     /translation="MEGEPGTSAFHRVLLKLSGQALGSETRAIDPATTKSIAGTLKVA
                     RSLDVDIAIVVGGGNIFRGMAAAAGGMDRTTGDHIGMMATVQNALALQDALEDEGVDT
                     RVMSAIEIRAICEPYIRRRAIRHLEKGRVVICAAGTGNPYFSTDSAAALRALELECEA
                     ILMAKRKYDGVYSADPDKDPSAEFIPEITHLQAIERGLKVMDTTALSLCMDNRMPIHV
                     FRMTGDNIVRVLSGERVGTVVRAPAGS"
     gene            982..1539
                     /gene="frr"
                     /locus_tag="VFZ86_08445"
     CDS             982..1539
                     /gene="frr"
                     /locus_tag="VFZ86_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011234093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEX5642352.1"
                     /translation="MEKELLADAKHRMHKAVEAVKNEFNTVRSGRASVGLLDRIHIDY
                     YGASTPLKQMANIAAPEPRLLTVQPFDKTAMKSIEKAIMESDLGLNPSSDGVVIRLPI
                     PALTEERRRDLVKLVHRLAEDGRVAVRNVRRDCMKDLKELAHEGEVGEDAEVRAEKEL
                     QKYTDDNVHQIDDLLKHKEAEILEV"
     gene            1580..2470
                     /gene="uppS"
                     /locus_tag="VFZ86_08450"
     CDS             1580..2470
                     /gene="uppS"
                     /locus_tag="VFZ86_08450"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HEX5642353.1"
                     /translation="MASLRQWLARWRLSRSTGAAEPAAGGATCRYLAIIMDGNGRWAQ
                     GRGLPVAAGHRAGAKALRRVLERALDLGIEEVTVYSFSTENWNRPKDEVEALMGLFVE
                     MMDTQVPDMHERGARVRFVGRREGAPDELVRRIEEAEALTAANTRMTLYIAFSYGGRR
                     ELLDAAEGLAAEYAASLGSAGETAPGAQDGAAGKPAPAFAEDDLRRQLYAPEMHDPEL
                     LIRTSGELRISNFLLWQCAYAELYFSDRYWPDFGPEDLDAALADYAGRQRRFGGRRAA
                     PAGGAGAGSSAADAGEDGAS"
     gene            2467..3249
                     /locus_tag="VFZ86_08455"
     CDS             2467..3249
                     /locus_tag="VFZ86_08455"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEX5642354.1"
                     /translation="MTTRIITGVVLGVLAVVVVVHGGLIFFLLMLALALLGLNEFYRL
                     TRKYRPLPLAGFLGVALMAGMAWFYSPFAVFGAVCAGVLFTALLGLLAGPKPGVTVRM
                     AVTVLGMLYVGLGFSAVLLMRQLDVGTAVLLTVIFGTWAGDTMAYFTGKFFGSTPMAP
                     VLSPKKTWEGFAGGAISTVLLVVFIGLYTPLGAADSLVLGAVIAVAGPLGDLFESLVK
                     RDVQIKDSGRGIPGHGGVLDRFDALIFSSVSAYFVLTLVLGF"
     gene            3399..4172
                     /gene="rfbF"
                     /locus_tag="VFZ86_08460"
     CDS             3399..4172
                     /gene="rfbF"
                     /locus_tag="VFZ86_08460"
                     /EC_number="2.7.7.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047343 - glucose-1-phosphate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate cytidylyltransferase"
                     /protein_id="HEX5642355.1"
                     /translation="MKVAILAGGLGTRLAEETQVKPKPMAEIGEHPILWHIMKIFGEQ
                     GYDEFVVCLGYKGYLVKEYFANYFLHNADVTFDLRGREMIVHENAAEPWRVTLVDTGA
                     ETQTGGRLRRVADYLGGERFMMTYGDGVADIDLSALLAVHEAQGRLATVTAVQPPGRF
                     GALEMDDAGTAVHDFVEKPRGDGGWMNGGFFVLEPAVLDYIAGDDTLWEKEPLERLAR
                     DGQLAAYRHYGFWQPMDTLRDKRALEELWESGAAPWKTW"
     gene            4157..>5183
                     /gene="rfbG"
                     /locus_tag="VFZ86_08465"
     CDS             4157..>5183
                     /gene="rfbG"
                     /locus_tag="VFZ86_08465"
                     /EC_number="4.2.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047733 - CDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glucose 4,6-dehydratase"
                     /protein_id="HEX5642356.1"
                     /translation="MEDLVMFGGAFSGRRVFVTGHTGFKGSWLTSWLLGLGAEVTGYA
                     LAPETEPSLFDALDLAARVRHVVGDVRDRDRLTRVMQEAGPSVVFHLAAQPLVRRGYA
                     EPHETFATNVMGTVGLLEAARACDAVDAVVIVTSDKCYDNADTGRAFRDGDPLGGRDP
                     YSASKGCAELVTASYRASFFDAGRPAGIASVRAGNVIGGGDWAPDRIIPDCVRALAAG
                     DPVVVRNPDAVRPWQHVLEPLAGYLSLAARLVDEGSGFAGAWNFGPADEDTSRPVRWV
                     VETFLNAWGEGSWATPQPGAQQPHEARSLALDSGKARERLAWAPVWDATAAVGHTATW
                     YREYYRRP"
BASE COUNT          795 a         1787 c         1856 g          745 t
ORIGIN      
        1 cagatcaagg gcaagcccga gaacgtggtc gagaagatca tcacgggcaa gctggagaag
       61 ttcttcggcg agacctgtct catggagcag gccttcgtca aggacccgga ccgcaccatc
      121 gagcaggtcc gcaccgatct cgtcggcgtc atcggcgaga acgtcgaggt gcgccgcttc
      181 gtgcgcttcc agctcggcga gacgacggta gcgggctgag ccccatggaa ggcgagccag
      241 gcacctccgc cttccaccgc gtcctcctca agctcagcgg acaggcgctg ggcagcgaga
      301 cgcgggcgat cgacccggcg acgacgaagt cgatcgccgg cacgctcaag gtcgcccgca
      361 gcctcgatgt cgacatcgcc atcgtcgtcg gcggcggcaa catcttccgt gggatggccg
      421 ccgccgccgg cggcatggac cgcaccaccg gcgaccacat cggcatgatg gcgacggtgc
      481 agaacgccct cgccctgcag gacgccctgg aggacgaggg cgtcgacacg agagtgatga
      541 gcgccatcga gatccgcgcc atctgcgagc cctacatccg ccgccgcgcc atccggcacc
      601 tcgagaaggg gcgcgtggtc atctgcgccg ccggcaccgg caacccctac ttcagcaccg
      661 actcggccgc ggccctgcgc gccctggagc tcgagtgcga ggccatcctc atggccaagc
      721 gcaagtacga cggcgtctac agcgccgacc ccgacaagga cccctcggcg gagttcatcc
      781 ccgagatcac ccacctgcag gcgatcgagc gcggcctcaa ggtcatggac acgaccgcgc
      841 tcagcctctg catggacaac cgcatgccga tccatgtgtt caggatgacc ggcgacaaca
      901 tcgtgcgcgt gctgtccggc gagcgcgtgg gcaccgtggt gcgcgcgccc gccgggagct
      961 gacccggcaa agggaggacc ggtggagaaa gagctgctcg ctgacgccaa gcatcgcatg
     1021 cacaaggccg tggaggccgt caagaacgag ttcaacaccg tgcgcagcgg ccgggccagc
     1081 gtcggcctgc tcgaccgcat ccacatcgac tactacggcg cctcgacgcc gctcaagcag
     1141 atggccaaca tcgccgcgcc cgagccccgg ctgctcaccg tgcagccgtt cgacaagacg
     1201 gcgatgaaga gcatcgagaa ggccatcatg gagagcgacc tcggcctcaa cccgagcagc
     1261 gacggcgtcg tgatccgcct gccgatcccc gcgctcaccg aggagcgccg ccgcgatctg
     1321 gtcaagctgg tgcaccggct ggcagaggac ggccgcgtcg ccgtgcgcaa cgtgcgccgc
     1381 gactgcatga aggacctcaa ggagctcgcc cacgagggcg aggtcggcga ggacgcggag
     1441 gtgcgcgccg agaaggagct ccagaagtac acggacgaca acgtccacca gatcgacgat
     1501 ctgctgaagc acaaagaggc ggagatcctc gaggtctgag ccgcctgccg ccggcgccga
     1561 ccctcaggac caccatccca tggcctcgct gcggcagtgg ctggcgcgct ggcgcctgag
     1621 cagatcgacc ggcgccgcgg agcctgcggc cggcggcgcc acctgccgct atctcgccat
     1681 catcatggac gggaacggcc gctgggccca agggcggggt ctgcccgtag cggccgggca
     1741 tcgggcaggc gcgaaggcgc tgcgccgcgt gctcgagcgt gccctcgacc tcggcatcga
     1801 ggaggtcacg gtgtactcgt tcagcaccga gaactggaac cggccgaagg acgaggtcga
     1861 ggcgctcatg ggactgttcg tcgagatgat ggacacgcag gtgcccgaca tgcacgagcg
     1921 cggcgcgcgc gtgcgcttcg tcgggcgtcg cgaaggggcg ccggacgaac tggtgcgccg
     1981 tatcgaggag gctgaggcgc tgaccgccgc caacaccagg atgacgctgt acatcgcctt
     2041 cagctacggc ggccggcgag agctgctgga cgcggccgag ggcctggcgg cggagtacgc
     2101 cgcctcgctg gggagcgcgg gggagaccgc gcccggtgcc caggacgggg cggccggaaa
     2161 gcccgcgcct gcgttcgccg aggacgacct ccgccgccag ctctacgcgc ccgagatgca
     2221 cgacccggag ctcctgatcc ggacctccgg ggagctgcgt atcagcaact tcctgctctg
     2281 gcagtgcgcc tacgcggagc tgtacttcag cgaccgctac tggccggact tcggccccga
     2341 ggacctggac gcggcgctcg ccgactacgc cggccgccag cgccgcttcg gcgggcgccg
     2401 ggcggcgccc gccggcgggg ccggcgccgg gtcgtccgcc gcggacgccg gcgaggacgg
     2461 cgcgtcgtga cgacgcgcat catcaccggc gtcgtcctcg gcgtgctcgc cgtcgtcgtg
     2521 gtcgtccacg gcgggctcat cttcttcctg ctcatgctcg ccctggcctt gctcgggctc
     2581 aacgagttct accggctcac caggaagtat cgccccctgc cgctggccgg cttcctcggc
     2641 gtcgcgctga tggccggcat ggcctggttc tacagcccgt tcgcggtgtt cggcgccgtg
     2701 tgcgccggcg tgctcttcac ggcgctgctc ggcctgctcg ccggccccaa gcccggcgtc
     2761 accgtgcgca tggcggtgac ggtcctcggc atgctctacg tcggcctcgg cttcagcgcc
     2821 gtgctgctca tgcggcagct ggacgtgggc acggccgtcc tgctcacggt gatcttcggc
     2881 acctgggccg gggataccat ggcgtacttc accggcaagt tcttcggcag cacgcccatg
     2941 gcgccggtgc tctcccccaa gaagacctgg gaggggttcg ccggcggcgc catcagcacg
     3001 gtgctgctgg tcgtcttcat cggcctctac acgccgctcg gagcggccga ctcgctcgtg
     3061 ctcggcgccg tgatcgccgt cgccggcccg ctcggcgacc tgttcgagag cctcgtgaaa
     3121 cgcgatgtgc agatcaagga ctccggccgc ggcatccccg gtcacggcgg cgtgctcgac
     3181 cgcttcgacg cgctgatctt ctcgtcggtc tcggcgtact tcgtgctcac gctcgtcctg
     3241 ggcttctagc ccgggcggag gctcgtgctc ctcacgggat caggccgctg cgctgtccgc
     3301 atcccgaggc gttgtcgtag aatcctcggg agtcgtgtcc ggggcggcgg cagccggccg
     3361 cgtcgcgcac gaagggaccg gggaggacgg gagacagcgt gaaagtagcc atcctcgcgg
     3421 ggggtctggg aacccgcctc gcggaagaga cgcaggtcaa gcccaagccc atggcggaga
     3481 tcggcgagca ccccatcctc tggcacatca tgaagatctt cggggagcag gggtacgacg
     3541 agttcgtcgt gtgcctcggc tacaagggct acctggtcaa ggagtacttc gccaactact
     3601 tcctgcacaa cgcagatgtg acgttcgacc tccgcggccg cgagatgatc gtccacgaga
     3661 acgccgccga gccttggcgc gtgaccctgg tggacaccgg ggcggagacg cagacaggcg
     3721 ggcgcctcag gcgggtggcc gactacctcg gaggcgagcg gttcatgatg acctacggtg
     3781 acggcgtcgc cgacatcgat ctctccgcgc tgctggccgt gcatgaggca cagggcaggt
     3841 tggcgacggt cacggcggta cagccaccag ggcgcttcgg cgccctcgag atggacgacg
     3901 ccggaacggc ggtgcacgat ttcgtggaga agcccagggg agacggcggg tggatgaacg
     3961 gcgggttctt cgtgctggag ccggccgtgc tcgactacat cgccggagac gacacgctct
     4021 gggagaagga gccgctggag cggctggctc gcgacgggca actggccgcg taccgtcact
     4081 acggcttctg gcagccgatg gacacgctgc gggacaagcg tgccctggag gagctgtggg
     4141 agtcgggtgc ggcgccgtgg aagacctggt gatgttcggc ggcgccttct ccggccggag
     4201 ggtcttcgtc accggccaca ccgggttcaa gggttcgtgg ctgacgtcgt ggctgctggg
     4261 cctgggggcg gaggtcacgg gctacgccct cgctccggag acagagccca gcctgttcga
     4321 cgcgctggac ctcgccgcgc gcgtgcggca cgtcgtcggc gatgtccgcg accgcgaccg
     4381 gctcacccgc gtgatgcagg aggcggggcc gtcggtggtc ttccacctgg ccgcgcagcc
     4441 tctggtgcgg cgtggctacg ccgagccgca cgagaccttc gcgaccaacg tcatgggcac
     4501 ggtcggcttg ctcgaggcgg cgcgcgcctg cgacgcggtc gacgcggtgg tgatcgtcac
     4561 cagcgacaag tgctacgaca acgcggacac cgggcgggcc tttcgtgacg gggatcccct
     4621 gggaggccgc gacccgtaca gcgccagcaa gggctgcgcg gaactggtca cggcctccta
     4681 ccgcgcgagc ttcttcgacg ccggccggcc ggcagggatc gcctcggtcc gcgccgggaa
     4741 cgtcatcggg ggaggcgact gggcgcccga ccgcatcatc cccgactgcg tgcgggccct
     4801 tgcggccggc gacccggtcg tcgtccgcaa cccggacgcg gtccggccgt ggcagcatgt
     4861 cctcgagccg cttgcgggct atctgtcgct ggccgctcgc ctcgtggatg aggggtccgg
     4921 gttcgccggg gcgtggaact tcgggcccgc cgacgaggac acgtcgcgtc cggtccgctg
     4981 ggtcgtcgag acgttcctga acgcctgggg ggagggatcg tgggcgacgc cgcagccggg
     5041 agcgcagcag ccacacgagg ctcggtcgct ggcgctcgac agcggcaagg cgcgagagcg
     5101 ccttgcctgg gctccggtat gggatgcgac ggcggcggtg gggcacaccg cgacctggta
     5161 tcgcgagtac tatcggcggc ccg
//
LOCUS       DASUMX010000169         9412 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_778036, whole genome shotgun sequence.
ACCESSION   DASUMX010000169 DASUMX010000000
VERSION     DASUMX010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 9412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9412
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_778036"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..943
                     /locus_tag="VFZ86_08470"
     CDS             <1..943
                     /locus_tag="VFZ86_08470"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5642357.1"
                     /translation="TPLPAGYRLHHPDPGTAAAVQAVLDAAESADSGEPRRHMTVVAT
                     QWEDPECRIERDWWVVAAPGGEIVGVAWLWPELASEVVADHYVHPGHRGLGLGEVLLD
                     TIESRVSELPPRGADGAPRQLVVWSEDADAARRRSLERRGFTAGRQYFEMALDLREAP
                     PAPPWPAGVEGRPFRGGVDDRRVHEVDVAAFAEHFLFKADSFETWRLWHLDAPNADPT
                     LWRLAWDGDRLVGFVMAVTSDEGALISSLAVLKPWRGRGIGRALLGAAFAVLRERGET
                     VVRLYVDAANVTDAVDVYEAAGMHVARRFDVMQKPLA"
     gene            complement(966..2063)
                     /locus_tag="VFZ86_08475"
     CDS             complement(966..2063)
                     /locus_tag="VFZ86_08475"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5642358.1"
                     /translation="MSWSRIRAIYRKDMRDALRDSRVLTALLFPLALGLVYSFIFPDE
                     IGNTEKIKVGIVSSTPTRLTSIIPREAGRTVDLTFVTMKDEAELERDVRSEEVDIGLV
                     IPPDFDADVRAGLSPVLITVLPSSPTLSGDVAVALLSRSVEALAAQPPPALIERRSLP
                     PETGGDTAVIDVLGARTQFILVATLMLLAMIAVYAVPTVLVEETEKKTTDALTLIAST
                     TDVIAAKALFGMGLSIVGVPVLFIITRADPAAPVPLAVAVALSALVLVGIGLLTTRVF
                     RTQQQVNTWSGVVMLVILAPAFTIGLSAPAIVNKILWLLPSVHTFRLIANAFAGRTLY
                     PGEGLSFGVLIAWALGAYGILWWSLARREDL"
     gene            complement(2060..2926)
                     /locus_tag="VFZ86_08480"
     CDS             complement(2060..2926)
                     /locus_tag="VFZ86_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5642359.1"
                     /translation="MTTAGASTAVTIEIEHLVKHYGEVRAVDGVSLSAREGEILGLLG
                     HNGAGKTTMIRMLTGRARPTSGTARVCGCDVVHERERMKPLINLVFEEQNLYERLSGR
                     ENLRLFAELYGLPKSRVEELLEAVRLREAATRKVKTYSSGMKQRLLVARALLNRPKVL
                     FLDEPTRGLDPTSAREVRDMVREQARKGTTVFLTTHYMEEADELCDRVAFLSSGRIVA
                     LDSPRELKLRYGERTAMVLLDTREEHTVGLDDPRDAERLQAWMAQGRVLTVHSREGTL
                     EDVFINLAGRPL"
     gene            complement(2940..3476)
                     /locus_tag="VFZ86_08485"
     CDS             complement(2940..3476)
                     /locus_tag="VFZ86_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642360.1"
                     /translation="MRKVSRAWYWLALAILAAGVLLPAVWGVTSVDGALEKADAFPRA
                     AVPGAVTVTVTDPGDQMIYFTGSGDQSPAALGLRVTDPAGASVPVTPYDLVVKLDLAG
                     DVGVAVATFPAAAEGAYVVTSTGNGAPEGAITVGTNVAMDTLPNVLSALAVMLLSVVT
                     AIAIVIVTLVRGSTRERS"
     gene            complement(3672..5378)
                     /locus_tag="VFZ86_08490"
     CDS             complement(3672..5378)
                     /locus_tag="VFZ86_08490"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX5642361.1"
                     /translation="MSFTIRNRLLLYFVCLVVFTAVAIAGITVVIASRDARERVVGQL
                     ESVATLKQQQVDSWIGGLRLNLDIVLSEEGIEQDLRTLTRAAPGAAERDAAYRRVLHR
                     FSWVATRVGLFEELLFMGPEGEVLVSTDRGHERQQLGMNDYFTEGMRGEFIQEPSWSL
                     SLNKMTVVASRPVGAGGEALGVLAGRANLDSLNAVMRGRAGLGQTGETYLVGSNHRLL
                     TELRTAGYTIPNAYVRTSGADAAVDGNESGSATYPGYAGQTVIGVYAWIPQLKVALMA
                     EQGEAEALKATRLALWTTGAVALLAALLAIIIGTTLIHSIVRPLSELGETAGRIADGE
                     LDLSARVKRDDEIGKLAGSFNRMTGRLRELAASKERQRLARDLHDAVSQTLFSISLMA
                     EVLPRVYEKDPAAGKQRLEELRELTRGALAEMRMLLLELRPAALAETSLPDLLRQLGE
                     AVVGRARIPVDVRIDEAVSPPPAVAVALYRIAQEALNNVVKHAAAGQATVTLRGSTGD
                     GHAGVELTVADDGCGFDIDDVRSGRLGLGIMAERAESIGALLELHSAPAQGTVVRVVW
                     SP"
     gene            complement(5450..6439)
                     /locus_tag="VFZ86_08495"
     CDS             complement(5450..6439)
                     /locus_tag="VFZ86_08495"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5642362.1"
                     /translation="MSLVSRSLLAALAVVLVLAPAAVLAACGNDSASPSPAASPDKVT
                     LQLNWFHEAEFVGYYVAAAKGFYGARDLDVTILEGGPGEPAIDSVLDGRADFAISSFG
                     EQRNAVAEDLPTVALMSAFQIPPLVIFSLAESGIASPADLAGKRVGVTTDYWKDVLDE
                     TLQAAGVKPSRVTAIPVEAGDLEPLYDGTVDAWLGYAQDEPIRAEVEGHRVRNVFPAD
                     YGVGGYEGLLLADRQTVEGAPELVRRFVQASQQGWRYAVEHPDEAARILLDWAPEPGL
                     EFQELAVRAVAPLVDAPQAVIGWIDAERWRLLMADDFDPANPGFTTLFSAAAP"
     gene            complement(6439..7053)
                     /locus_tag="VFZ86_08500"
     CDS             complement(6439..7053)
                     /locus_tag="VFZ86_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5642363.1"
                     /translation="MLVDDHAVVRSGLSAFLLAFDDLEYVGEAAGGAEAVERCLVLKP
                     DVILMDLVMPDVDGAEATRRIKEACPHVQVIALTSYKEDDLVQGALKAGALSYLLKNV
                     TADELAGAIRAAHAGRPILAPEAAEVLIKAATAPAAVEAEGLTTRELEILRLMVSGES
                     NPDIAARLFVSRSTVKFHVSNILMKLGATSRTEAVAIALQKGLV"
     gene            7320..7772
                     /locus_tag="VFZ86_08505"
     CDS             7320..7772
                     /locus_tag="VFZ86_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642364.1"
                     /translation="MDAMKHEAQRSRRAVVAEREPTVRSALRALTTQGLGMQVIGEAC
                     DEAALHRQVQALRPDLVIVAWDLVDDHAEAALAALRGASEGLRVVVLGLRPETRPQAL
                     TAGADAYISMVDAPDVVTRVLQTCPRPAGSGHTRADEKTNEPGGPSWD"
     gene            7799..>9412
                     /locus_tag="VFZ86_08510"
     CDS             7799..>9412
                     /locus_tag="VFZ86_08510"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX5642365.1"
                     /translation="MSQHTDTTTPPRGGRSGGFTERLARLSALHPWRTIGLWAVLIVL
                     AVAAVGSLLGTGLTSDMKFRAAKPDSLVGQELLEQRLTGPRQITDFVIVRSATMTVDA
                     PSFKAYVDSLAGRITALGPDVVKGVSTYYQTKDPTAVSKDRHATLIPVVMAGDIDDAL
                     ANVDELHETALSLERHGFTVAQTGDASINEMFTKLAEKDLQRGEIFGVPAALIVLLLV
                     FGAVLAACMPILLSIISIILALAITALIGQVYPMNTFVLNILTMMGLAVGIDYTLFVI
                     SRYREERARGLAKIDAISATGATANRAIFFSGMTVVLAMTGMVIIPLDIMISMGVGVM
                     LVVLTTLLTALIALPALLSLLGDKVDALRLPFIGRRAARHLNGHDGIWERLSHSIMRR
                     PVVWLTVAVTVLLVLAAPVVMMKEGNNTASAATLPANEYAKQGWDQLDRDFSLGKANP
                     VMIVVDGRVDSPAVGLAIGRLKAAMTADGAFGPSQVTANEKGDLTLITTTLAGDPAGE
                     ATQNLVKRLREDIVPRAAGDVASKIYVTGN"
BASE COUNT         1399 a         3357 c         3317 g         1339 t
ORIGIN      
        1 gaccccactg ccggcgggct acaggctgca tcatcccgac cccgggacgg cggcggccgt
       61 ccaggcggtc ctcgacgccg ccgagagcgc cgacagcgga gagccgcgcc ggcacatgac
      121 cgtcgtcgcg acccagtggg aggatcccga gtgccgcatc gagcgcgact ggtgggtggt
      181 cgcggccccc ggcggcgaga tcgtcggcgt cgcctggttg tggccggagc tggcctccga
      241 ggtcgtcgcc gaccactacg tgcacccggg gcatcgcggg ctcggcctcg gggaggtcct
      301 cctcgacacc atcgagtcgc gcgtctccga actgccgccg cggggtgcgg acggagctcc
      361 gcggcagctc gtcgtgtgga gcgaggacgc ggacgctgcg cggcgccgct cgctcgaacg
      421 ccgcggcttc acggccggac ggcagtactt cgagatggct ctcgatctgc gcgaagctcc
      481 tccggcgccg ccctggcccg cgggtgtgga gggacgcccg ttccgcggcg gcgtcgacga
      541 caggcgggtg cacgaggtcg acgtcgcggc gttcgcagag cacttcctct tcaaggccga
      601 ctccttcgag acctggcggc tctggcacct cgacgcgccc aacgccgacc ccacgctgtg
      661 gcggctcgcc tgggacggcg acaggctcgt cgggttcgtc atggccgtca cgtccgacga
      721 gggagccctc atctcgagcc tcgcggtgct caagccgtgg cgcggccggg gcatcggccg
      781 cgccctgctc ggcgccgcgt tcgcagtgct gcgcgagcgc ggcgagaccg tcgtgcgcct
      841 ctacgtggac gcggcgaacg tgaccgatgc cgtggacgtc tacgaggcgg caggcatgca
      901 cgtggcacgc cggttcgacg tgatgcagaa gccgctcgcc tgacgcgcca cgccccgagc
      961 agcgctcaga gatcctcgcg ccgcgccaga ctccaccaga ggatgccgta cgcccccagt
     1021 gcccacgcga tgaggacgcc gaaggagagc ccctctccgg ggtagagcgt gcgcccggcg
     1081 aacgcgttgg cgatgagccg gaaggtgtgg acggagggga gaagccagag gatcttgttg
     1141 acgatcgccg gcgccgacag gccgatggtg aatgcgggag cgaggatgac cagcatcacg
     1201 acaccgctcc acgtgttcac ctgttgctgc gtcctgaaga cccgcgtggt caggagcccg
     1261 ataccgacca gcacgagggc cgagagcgcg accgccacgg ccagaggcac gggagcggcg
     1321 gggtcagcac gggtgatgat gaacagcacg ggcaccccaa caatgctgag ccccatcccg
     1381 aagagcgcct tggccgcgat cacgtccgtg gtcgaggcga tgagggtcag ggcgtccgtc
     1441 gtcttcttct cggtctcctc cacgaggacg gtggggaccg cgtacacggc gatcatggcc
     1501 agcagcatga gcgtggcgac gaggatgaac tgggtgcgcg ccccgagaac gtcgatgacg
     1561 gcggtgtcgc cgccggtctc cggcggcagc gagcgtcgct cgatgagggc cggcggcggc
     1621 tgcgcggcca gagcctcgac gctgcggctg agcagagcga ccgcgacgtc cccgctcagc
     1681 gtgggcgatg atggcaggac ggtgatcagc accggcgaga ggccggcgcg cacgtcggcg
     1741 tcgaagtccg gcgggatcac gaggccgatg tcgacctcct cgctccgcac gtcgcgttcc
     1801 agctcggcct cgtccttcat ggtcacgaag gtcaggtcca ctgtccgacc ggcctcgcgg
     1861 gggatgatgg acgtgagcct ggtaggggtg ctcgagacga tgcccacctt gatcttctcc
     1921 gtgttcccga tctcgtcggg gaagatgaag gagtagacca gccccagtgc gagcggaaag
     1981 aggagcgcgg tgaggacgcg gctgtcgcgg agcgcgtccc gcatgtcctt gcggtagatg
     2041 gcgcggatgc gtgaccagct cacaggggcc tccccgcaag gttgatgaac acgtcctcga
     2101 gcgtgccctc ccgggagtgc acggtgagga cccgcccctg ggccatccat gcctggaggc
     2161 gttccgcgtc ccgcgggtcg tcgagaccca ccgtgtgctc ctcgcgcgtg tcgagcagca
     2221 ccatcgccgt ccgctcccca taccggagct tgagctctcg cgggctgtcg agggcgacga
     2281 tgcgtccgga ggagaggaag gcgacgcgat cgcagagctc gtcggcctcc tccatgtagt
     2341 gcgtggtgag gaagaccgtc gtccccttgc gggcttgctc gcgcaccatg tcgcgcactt
     2401 cgcgcgccga ggtgggatcg aggccgcgcg tgggctcgtc gaggaacagc accttgggcc
     2461 ggttgagcag ggcacgggcc acgagcaggc gctgcttcat gccgctcgag taggtcttca
     2521 ccttgcgcgt cgcggcttcc cgcaggcgca cggcctcgag aagctcctcg acgcgggact
     2581 tgggaaggcc gtagagctcg gcgaagagtc gcaggttctc gcggccgctg agccgctcgt
     2641 agaggttctg ctcctcgaag accaggttga tgagcggctt catgcgttcg cgctcgtgca
     2701 cgacgtcgca gccgcagacc ctggccgtgc cgctcgtcgg ccgcgcccgg cccgtgagca
     2761 tccggatcat cgtcgtcttg cccgccccgt tgtggccgag caggccgagg atctcgccct
     2821 cgcgcgccga caggctgacg ccgtcgacgg cgcgcacctc gccgtagtgc ttgaccaggt
     2881 gctcgatctc gatggtgacg gctgtcgagg ctcctgccgt cgtcacggca cttctccggt
     2941 catgatctct cccgggtcga cccgcggacg agcgtgacga tcacgatggc gatcgcggtg
     3001 accacgctga gcaacatgac cgcgagggcg ctgaggacgt tcgggagcgt gtccatggcc
     3061 acgttggtcc caacggtgat cgcgccttcg ggggcgccgt tccccgtgga ggtcacgacg
     3121 taggcgccct cggcggcggc ggggaacgtg gcgacggcca cgcccacgtc accggcgaga
     3181 tccagcttca cgacgaggtc gtacggcgtg acgggcacgg aggcgccggc cgggtccgtg
     3241 acccgcagcc ccagcgccgc cgggctctgg tctcccgacc cggtgaagta gatcatctgg
     3301 tccccggggt ccgtgacggt gacggtcacc gcgccgggga ccgccgcacg cgggaacgca
     3361 tcggccttct cgagcgcgcc gtccacgctc gtcacgcccc agaccgccgg cagcagcacg
     3421 ccggcggcga ggatcgccag ggcgagccag taccacgccc gggagacctt cctcacgcct
     3481 ccgtcagatg gcgcgacgcg tcgtgctggg atgactgccg acgtggtgtc catgactccc
     3541 ccttgggatc ccgagttgac tccggcacca cgctacggcc gcgtgctcgt gagcgcgacg
     3601 gccagacagg gggtcttctc agctggccgt gcggtgaggg ccggcccggc cacctggcca
     3661 gggccgtgag gtcacggcga ccagacgacg cgcaccacgg tgccctgcgc gggcgccgag
     3721 tgcagctcga gcagggcgcc gatggattcg gcgcgctcgg ccatgatgcc gaggcccagg
     3781 cggcctgagc gcacgtcgtc gatgtcgaac ccgcagccgt cgtccgcgac ggtgagctcc
     3841 acgccggcgt ggccgtctcc ggtcgagccg cgcaacgtca ccgtcgcctg cccggccgcc
     3901 gcgtgcttga ccacgttgtt cagggcctcc tgcgcgatgc ggtacagggc gaccgcgacc
     3961 gcgggcggcg ggctcaccgc ctcgtcgatc cggacgtcga cggggatcct ggcgcgtccc
     4021 accaccgcct cgcccagctg gcggagcagg tcgggcagac tcgtctccgc cagcgcggcg
     4081 gggcgcagct cgagcagcag catgcgcatc tccgcgagcg ccccccgcgt gagctcccgg
     4141 agctcctcca gccgctgctt gccggccgcg gggtccttct cgtacacccg cggcaacact
     4201 tcggccatca ggctgatgga gaagagcgtc tggctcacgg cgtcgtgcag gtcgcgcgcg
     4261 agccgctgcc gctccttgct cgcggcgagc tcgcggagcc ggcccgtcat gcggttgaag
     4321 gagccggcga gcttcccgat ctcatcgtcc cgcttgaccc gggcgctcag atccagctcc
     4381 ccgtcggcga tgcggccggc cgtctctccc agctccgaca gcggccggac gatgctgtgg
     4441 atgagcgtcg tcccgatgat gatggccagg agcgccgcca ggagcgcgac cgcgccggtg
     4501 gtccacaagg cgagccgcgt ggccttgagg gcctcggcct cgccctgctc ggccatgagc
     4561 gccaccttga gctgcgggat ccacgcgtag acgccgatca ccgtctgccc ggcgtacccc
     4621 gggtaggtcg ccgagccgct ctcgttcccg tccacggccg cgtcggcccc gctcgtgcgc
     4681 acgtaggcgt tcgggatcgt gtagcccgcg gtgcgaagtt ccgtcagcag acggtggttc
     4741 gagcccacga gataggtctc gccggtctgc ccgagcccgg cgcggccgcg catcacggcg
     4801 ttgaggctgt cgagattggc gcggccggcg aggacgccca gcgcctcgcc gccggccccg
     4861 acggggcggc tcgcgacgac ggtcatcttg ttcagggaga gcgaccacga gggctcctgg
     4921 atgaactcgc cccgcatgcc ctcggtgaag tagtcgttca tgccgagctg ctgtcgctcg
     4981 tgccctctgt cggtcgagac gagcacctcg ccctcgggcc ccatgaagag gagctcctcg
     5041 aacagcccga cgcgggtggc gacccaggag aaccggtgca ggacgcggcg ataggcagcg
     5101 tcgcgctcgg cggcgccggg agcggcccgg gtgagggtgc gcagatcctg ctcgatcccc
     5161 tcctcggaca ggacgatgtc gaggttcagg cggaggcctc cgatccagga gtccacctgc
     5221 tgctgcttca gcgtggccac ggactcgagc tggcccacga cccgttcgcg ggcgtcgcga
     5281 gaggcgatga cgaccgtgat gccggcgatg gcgacggcgg tgaagacgac cagacagacg
     5341 aagtagagga ggagcctgtt cctgatggtg aaggacatgg gaaggcggga gcggagcaag
     5401 atgccgttcc ttcagcggag cgcgggatgc acgtcgagcc tggaggcggt cagggtgcgg
     5461 cggccgagaa gagggtggtg aagcccgggt tcgccggatc gaagtcgtcg gccatcagca
     5521 ggcgccagcg ctcggcgtcg atccacccga tcaccgcctg cggcgcgtcc acgagggggg
     5581 cgaccgcgcg caccgccagc tcctggaact cgaggcccgg ctccggcgcc cagtcgagca
     5641 ggatccgcgc cgcctcgtcc gggtgctcga ccgcgtagcg ccagccctgc tggctcgcct
     5701 gcacgaagcg gcggacgagc tcgggggcgc cctccacggt ctgccggtcc gccagcagca
     5761 ggccctcgta gccgcccacc ccgtagtcgg cggggaagac gttcctcacc cggtgcccct
     5821 cgacctcggc gcggatgggc tcgtcctgcg cgtacccgag ccaggcgtcc accgtgccgt
     5881 cgtacagcgg ctccagatcg cccgcctcca ccgggatcgc agtcacccgc gacggcttca
     5941 cgccggcggc ctgcagcgtc tcgtccagca cgtccttcca gtagtcggtc gtgaccccca
     6001 cacgcttccc cgcgaggtcg gcagggctgg cgatgcccga ctcggccagg gagaagatga
     6061 ccagcggagg gatctggaag gcggacatca gcgcgaccgt cggcaggtcc tcggccacgg
     6121 cgttccgctg ctccccgaac gaggagatgg cgaagtccgc gcgaccgtcg agcacggagt
     6181 cgatcgcggg ctcgccgggc ccgccctcga ggatggtgac gtcgaggtct ctggccccgt
     6241 agaacccctt ggccgcggcc acgtagtagc cgacgaactc cgcctcgtgg aaccagttca
     6301 gctgcagcgt gaccttgtcg ggggaggccg ccggcgaggg cgacgcggag tcgttgccgc
     6361 aggcggcgag gacggccgcc ggcgccagga cgaggacgac ggccagcgcg gcgagcagcg
     6421 accggctgac gaggctcatc agacgagccc cttctgcagc gcgatcgcga ccgcctcggt
     6481 gcgggaggtg gccccaagct tcatgaggat gttgctgacg tggaacttga ccgtggaccg
     6541 gctgacgaag aggcgtgccg cgatgtccgg gttgctctcg cccgacacca tgagccggag
     6601 gatctcgagc tcccgggtgg tgagcccctc cgcctcgacg gccgccggtg cggtcgccgc
     6661 cttgatgagc acttcggccg cctccggggc gaggatcgga cggccggcgt gcgcggcgcg
     6721 gatggcgccg gccagttcgt ccgcggtgac gttcttgagc aggtagctca gggcgccggc
     6781 cttgagcgct ccctgcacca ggtcgtcctc cttgtagctg gtgagggcga tcacctggac
     6841 gtggggacag gcctccttga tgcggcgggt ggcctcggcg ccgtccacgt ccggcatgac
     6901 gagatccatg aggatcacgt ccggcttgag gacgaggcac cgctccacgg cttcggcgcc
     6961 gccggcggcc tcacccacgt actcgaggtc gtcgaaggcg aggaggaacg cggaaaggcc
     7021 gctccggacg acggcgtgat cgtcgacgag catgacccgg atgggctccg actctgctat
     7081 ctcccctctc ccttccggcc cccgtcggcg tgctggcacc ggcccgccgt cgaccacgcg
     7141 tggagcgcgg acgggcactt ggatgccagc cctacatgct agacgccgga gcgcgctcag
     7201 ccaagcccag tgtgcgaccc ctggccagcc ggccagtgaa aggaccggtc acctcaccag
     7261 ccacaggtct cccccgccgg cgggcgtcaa cgaaggcccg ccgccctagc cttggaccca
     7321 tggacgcaat gaagcacgaa gcgcagcgga gcagacgcgc cgtagtggcc gagcgagagc
     7381 ccacagtacg gagcgccctg cgcgcgctga cgacccaggg gctcggcatg caggtgatcg
     7441 gcgaggcctg cgatgaagcg gcgctccacc gccaggtgca ggcgctgcgc ccggacctcg
     7501 tgatcgtggc ctgggacctg gtggacgacc acgccgaggc ggcgctcgcc gccctgcgcg
     7561 gcgcgtcgga gggcctgcgc gtcgtcgtcc tcggcctccg gccggagacg cgcccgcagg
     7621 cgctcaccgc cggcgccgac gcctacatca gcatggtgga cgcgcccgac gtcgtgacgc
     7681 gcgtcctgca gacgtgcccg cgaccggccg ggtccggcca cacgcgggcc gacgagaaga
     7741 cgaacgagcc agggggaccg tcatgggact gagagcaagg cacacgggag gagcgaccat
     7801 gtcgcagcac acggacacga cgacgccgcc gcggggcggc aggagcggcg gcttcacgga
     7861 gcgcctggcg cgcctcagcg ccctccaccc gtggcgcacc atcggcctct gggcggtgct
     7921 catcgtcctg gccgtggccg cagtcggttc gctgctcggc acaggcctca cctcggacat
     7981 gaagttccgc gccgccaagc cggacagcct ggtcggccag gagctcctcg agcagcgcct
     8041 caccggaccg cggcagatca ccgacttcgt gatcgtgcgg tcggcgacga tgaccgtcga
     8101 cgcgccctcc ttcaaggcgt acgtcgacag cctcgccgga cggatcaccg ccctcgggcc
     8161 ggacgtggtc aagggcgtgt ccacctacta ccagaccaag gatccgacgg ccgtgagcaa
     8221 ggaccggcat gccaccctga tccccgtcgt gatggccggc gacatcgacg acgcgctcgc
     8281 caacgtcgac gagctccacg agacggccct ttcactcgag cgtcacggct tcaccgtcgc
     8341 gcagaccggc gacgccagca tcaacgagat gttcaccaag ctggcggaga aggacctgca
     8401 gcgcggcgag atcttcggcg tccccgccgc gctcatcgtg ctcctcctcg tcttcggcgc
     8461 cgtgctcgcc gcgtgcatgc cgatcctgct cagcatcatc agcatcatcc tggccctggc
     8521 catcaccgcg ctcatcggcc aggtctaccc gatgaacacc ttcgtcctga acatcctcac
     8581 catgatgggc ctggccgtcg gcatcgacta cacgctgttc gtcatcagcc gctaccgcga
     8641 ggagcgcgcc cgcggcctcg ccaagatcga tgccatctcc gccaccggcg ccaccgccaa
     8701 ccgcgccatc ttcttcagcg gcatgaccgt ggtgctggcg atgaccggca tggtcatcat
     8761 cccgctcgac atcatgatca gcatgggcgt cggcgtgatg ctcgtcgtac tcaccacgct
     8821 gctcacggcg ctcatcgccc tcccggccct cctgagcctg ctcggcgaca aggtcgacgc
     8881 cctgcgcctg ccgttcatcg gccgccgggc cgcccgccat ctcaacggtc atgacgggat
     8941 ctgggagcgc ctctcgcaca gcatcatgcg gcgccccgtg gtctggctca ccgtcgccgt
     9001 caccgtcctg ctcgtcctgg ccgcccccgt ggtcatgatg aaggagggca acaacacggc
     9061 cagcgccgcg accctgccgg ccaatgagta tgccaagcag ggttgggacc agctcgaccg
     9121 cgacttcagc ctcggcaagg ccaacccggt catgatcgtc gtcgacggcc gcgtggactc
     9181 tccagcggtc gggctggcga tcgggcggct gaaggcggcg atgacggccg acggcgcctt
     9241 cgggccctcc caggtcaccg cgaacgagaa gggcgacctc acgctcatca ccaccacgct
     9301 ggcgggcgac cccgccggcg aggcgaccca gaacctcgtc aagcggctgc gcgaggacat
     9361 cgtcccgcgg gccgccggcg acgtcgcctc gaagatctac gtcaccggca ac
//
LOCUS       DASUMX010000170         2690 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1640158, whole genome shotgun sequence.
ACCESSION   DASUMX010000170 DASUMX010000000
VERSION     DASUMX010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1640158"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..876
                     /locus_tag="VFZ86_08515"
     CDS             <1..876
                     /locus_tag="VFZ86_08515"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEX5642366.1"
                     /translation="DPAVAAAPLALAVKPKVTVSHGTLVPFLPSRFVVKVAPTAYDGV
                     IVLKVVHRRALVGTYRARVKDGRAVFKAPLRGVDGFTLTYVVPAAGGLTGRTVQSKVS
                     VRARTLSAGSSGPYVKGMLTALQRLRFRVPNVGTSFTAQVKDSVMAFQKAYRLPRTYV
                     FNSECWRKLDGAKVIKPRYAKPSTHIEVDKARQILMIVKGGKPYGIICVSTGATGNTP
                     EGTFRIQQKHPYTSSGFGGTLVRTMGFLGDFAIHGWADVPPYPASHGCVREPIWACYW
                     TYDNSWVGETVYIYR"
     gene            complement(914..1822)
                     /locus_tag="VFZ86_08520"
     CDS             complement(914..1822)
                     /locus_tag="VFZ86_08520"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HEX5642367.1"
                     /translation="MRTSLIAVPYDLGRAEVGSGRGPGAYLKAGAAEALRARGHEVEV
                     VTVRREAPFVSELDAVLEVDRAVAAAVTAALRDDALPLVVAGNCNVTLGVRAGLQHGG
                     GADAALVWLDAHGDFNTPATSETGYLDGMPLAMLCGRAYREAVEHQLVMAPLREATVV
                     HAGGRDFDREELESLLASAVRVVDGGELRGRGPAEALAPSLDALAAERREAMRLDPEA
                     RPPAHLHVDIDVLDPTAAPGVAFPTPRGLSAAQLLEVIDLVRERFALAALTLASYTPD
                     RDEAGITLAAGLAVLQRATTELAHRR"
     gene            complement(1830..2288)
                     /locus_tag="VFZ86_08525"
     CDS             complement(1830..2288)
                     /locus_tag="VFZ86_08525"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5642368.1"
                     /translation="MERVKCEIREASRDDRGMLLLLAEETLHPLAEGAGHPERYHAGD
                     MIALLERADVFVAECGGEPGGFVAAETDEDACAVRCICVNPGFEARGVANQLLDWVEG
                     LAIERRLSRLVAHVPATDTPSLRLYRGHGFSGHEEDDEMLALEKRLPAAD"
     gene            complement(2409..>2690)
                     /locus_tag="VFZ86_08530"
     CDS             complement(2409..>2690)
                     /locus_tag="VFZ86_08530"
                     /inference="COORDINATES: protein motif:HMM:NF013962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX5642369.1"
                     /translation="EPAAQDLGGRYKTSIVCSMDQDEPGSLLLILSEFAYRHVNLAKI
                     ESRPSKKSLGHYVFFIDIEGSIESQPVAQALKCLACKLPWIKVLGSYPA"
BASE COUNT          364 a         1005 c          945 g          376 t
ORIGIN      
        1 gacccggcgg tggccgccgc gcctctggcg ctcgcggtga agcccaaggt gaccgtttcg
       61 cacggcacgc tggtgccctt cctaccgtcg aggttcgtcg tcaaggtcgc gccgaccgcc
      121 tacgacggcg tgatcgtgct caaggtcgtg catcgccgcg ccctcgtggg cacctacagg
      181 gcgcgcgtca aagacgggcg cgccgtcttc aaggccccgc tccgcggcgt cgacggcttc
      241 acgctcacct acgtggtgcc ggccgccggc ggcctcaccg ggcgcaccgt gcagtccaag
      301 gtgtcggtgc gcgccaggac gctctccgcc gggtcctcgg gcccctacgt caagggaatg
      361 ctcacggcgc tgcagcgcct gcgcttccgc gtccccaacg tcggcaccag cttcaccgcc
      421 caggtcaagg actcggtgat ggccttccag aaggcctacc gcctgccgcg cacctatgtc
      481 ttcaacagcg agtgctggcg caagctggac ggggccaagg tcatcaagcc ccgctacgcg
      541 aagccctcca cgcacatcga ggtcgacaag gcccgtcaga tcctcatgat cgtcaagggc
      601 ggcaagccgt acggcatcat ctgcgtctcc accggggcga ccggcaacac gccggagggt
      661 acgttccgca tccagcagaa gcacccgtac acgagctcgg ggttcggcgg caccctggtc
      721 cggaccatgg gcttcctcgg cgacttcgcc atccacgggt gggcggacgt gccgccgtat
      781 ccggccagcc acggctgcgt gcgcgagccc atctgggcct gctactggac ctacgacaac
      841 tcctgggtcg gcgagaccgt ctacatctat cgctgacgcg ccggcgcgtt gacgaaggac
      901 cgggcgccgg ccgctatctg cggtgcgcca gctccgtggt cgcgcgctgg agcaccgcga
      961 ggccggcggc cagcgtgatc ccggcctcgt ccctgtccgg cgtgtacgag gcgagcgtga
     1021 gcgcggcaag ggcgaagcgc tcgcgcacca ggtcgatgac ttccagcagc tgagccgcgg
     1081 acaggccgcg cggcgtgggg aaggcgacgc cgggcgccgc cgtcgggtcg agcacgtcga
     1141 tgtcgacgtg caggtgcgcc ggcggcctgg cctccgggtc gagccgcatc gcctcgcggc
     1201 gctccgcggc gagcgcgtcc agcgatgggg cgagggcctc ggccggtccc cggccgcgca
     1261 gctcgcctcc gtccacgacg cgcacggccg aggcgagcag cgactccagc tcctcgcggt
     1321 cgaagtcgcg gccgccggcg tgcaccaccg tcgcctcgcg cagcggcgcc atcaccagct
     1381 ggtgctcgac cgcctcacgg taggcgcggc cgcagagcat ggccagcggc atgccgtcga
     1441 ggtagccggt ctcgctggtg gccggcgtgt tgaagtcccc gtgcgcgtcg agccagacga
     1501 gggcggcgtc cgcgccgccg ccgtgctgca gcccggcgcg gaccccgagc gtcacgttgc
     1561 agttgccggc caccacgagc gggagcgcgt cgtcgcgcag cgccgccgtc actgccgcgg
     1621 ctacggcgcg gtccacctcg agtaccgcgt cgagctcgga cacgaacggc gcctcgcgcc
     1681 gcacggtgac gacctcgacc tcgtggccgc gggcacgcag cgcctccgcg gccccggcct
     1741 tgaggtaggc tccggggccg cgcccgctgc cgacctcggc acggccgagg tcgtagggga
     1801 cggcgatgag agaggtacgc accggcgcct cagtccgccg ccggcaggcg cttctcgaga
     1861 gcgagcatct cgtcgtcctc ctcgtgaccg ctgaagccgt gcccgcggta caggcgcagc
     1921 gacggagtgt ccgtcgccgg cacgtgggcc acgagacgcg acagcctgcg ctcgatcgcc
     1981 aggccctcga cccagtccag cagctggttg gcgacgccgc gcgcctcgaa ccccgggttc
     2041 acgcagatgc agcggaccgc gcaggcgtcc tcgtcggtct ccgcagcgac gaacccgccg
     2101 ggctcgccgc cgcactccgc cacgaagacg tcggcgcgct cgagcagcgc gatcatgtcg
     2161 ccggcgtggt agcgctcggg gtggccggcg ccctcggcga gcgggtgcag ggtctcctcc
     2221 gcgagcagca gcagcatccc gcggtcgtcc cggctcgcct cgcggatctc acacttgacg
     2281 cgctccatgc gcacctccgg aagacgcttc ctgcggccgg accgccgtga cgatcgcggc
     2341 cgcctggtcg tgagggtggg gaggggcggc cgcaccggct cgccccggcc cggtgtccgc
     2401 ccgccggctc atgccggata cgaccccagc accttgatcc acggcagctt gcaggcgagg
     2461 cacttcagcg cctgcgccac cggctggctc tcgatgcttc cttcgatgtc gatgaagaag
     2521 acgtagtggc ccagcgactt cttggacggc cgcgactcga tcttggccag gttgacgtgc
     2581 cggtaggcga actcgctgag gatgagcagc aggctgccgg gctcgtcctg gtccatgctg
     2641 cagacgatgc tcgtcttgta gcggccgccg aggtcctgcg cggccggctc
//
LOCUS       DASUMX010000171         2026 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1353570, whole genome shotgun sequence.
ACCESSION   DASUMX010000171 DASUMX010000000
VERSION     DASUMX010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1353570"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..131
                     /locus_tag="VFZ86_08535"
     CDS             <1..131
                     /locus_tag="VFZ86_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019694396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEX5642370.1"
                     /translation="AGGASVALTWAWAVFHILIILLQAFIFMVLSVVYVAMAHEHH"
     gene            189..455
                     /gene="atpE"
                     /locus_tag="VFZ86_08540"
     CDS             189..455
                     /gene="atpE"
                     /locus_tag="VFZ86_08540"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012357116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEX5642371.1"
                     /translation="MAQYFALVQAYTALGIGIIIGLGAIGACIGIGIMGSKFLEAAGR
                     QPELIPQLQKFMFLLAGLIDAAFLIGVGIAMFFGFANPLLSAVK"
     gene            484..954
                     /locus_tag="VFZ86_08545"
     CDS             484..954
                     /locus_tag="VFZ86_08545"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEX5642372.1"
                     /translation="MNINATLFVQALSFAFLIWFSVKFIWPPLNRAIDERNKRIADGL
                     AAAEKGKASLVEAQAKGESAMKEARERASGLLGDSEKRGAQIVEEAKAAAKEEADRII
                     AGAREQIASEVAKAKTELREQVAALAVAGAEKILQREIDAKSHAEMLGKLKAQL"
     gene            964..1500
                     /locus_tag="VFZ86_08550"
     CDS             964..1500
                     /locus_tag="VFZ86_08550"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004402.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEX5642373.1"
                     /translation="MAEIATLARPYAEASFRSAQESGDLAGWSGGLALAGAIAADPQM
                     ADVLGNPRFTRAQKLELFFGVGGDGLSAPVRNLVTILVDGARAVLLPEISAQFDALKR
                     EHESVLKVRIVSARPLADAERDDLVAEVGRRYKRTVEATVEIDASLIGGARVHIGDKV
                     IHASMRDALAQMAVALTR"
     gene            1530..>2026
                     /locus_tag="VFZ86_08555"
     CDS             1530..>2026
                     /locus_tag="VFZ86_08555"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763893.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEX5642374.1"
                     /translation="MQINPAEISELIKSKIQNLQVSAEKRTEGTVISVTDGICRVHGL
                     TDVMQGEMLEFPGNTFGTALNLERDSVGAVVLGSYEHISEGDTVKCTGRILEVPVGPE
                     LIGRVVNSLGQPIDGKGPIDAKMTEPIEKVAPGVIWRKSVSQPVQTGLKAIDAMVPVG
                     RGQREL"
BASE COUNT          361 a          703 c          661 g          301 t
ORIGIN      
        1 tcgccggcgg cgcctccgtg gccctcacct gggcatgggc cgtgttccac atcctgatca
       61 tcctgctgca ggcgttcatc ttcatggtgc tgtcggtcgt gtacgtcgcg atggcccacg
      121 agcaccacta gaaaacgaat cgactcactt cctcaacttc aacacccgca cctccgaaag
      181 gaaaaacaat ggctcagtac ttcgccctcg tccaagccta cacggcgctc ggcatcggca
      241 tcatcatcgg cctgggcgcc atcggcgcct gtatcggcat cggcatcatg ggctccaagt
      301 tcctggaagc cgccggccgc cagcccgagc tcatcccgca gctgcagaag ttcatgttcc
      361 tgctcgcggg cctgatcgac gcggcgttcc tgatcggcgt cggcatcgcg atgttcttcg
      421 gcttcgcgaa cccgctcctt tcggccgtca agtaatcagg ttccaacgga agggtcgttc
      481 gccatgaaca tcaacgccac cttgttcgtc caggcgctgt cgttcgcgtt cctcatctgg
      541 ttctcggtca agttcatctg gccgccgctc aaccgtgcca tcgacgagcg caacaagcgg
      601 atcgccgacg ggctcgcggc cgccgagaaa ggcaaggcct cgctggtcga ggcccaggcc
      661 aagggcgaga gcgcgatgaa ggaagcgcgc gagcgggcct cgggcctgct gggagacagc
      721 gagaagcgcg gcgcgcagat cgtcgaggag gcgaaggctg ccgccaagga agaggccgac
      781 cgcatcatcg ccggcgcgcg cgagcagatc gcctccgaag tcgccaaggc gaagacggag
      841 cttcgcgagc aggtcgccgc actcgcggtg gccggcgccg agaagatcct gcagcgcgaa
      901 atcgacgcga agtcgcacgc cgagatgctc ggcaagttga aggcccagct ctaggacccc
      961 cccatggccg aaatcgccac cctcgcccgc ccctacgccg aagcgtcgtt ccgctcggcg
     1021 caggagtccg gggacctcgc gggatggtcc ggcgggctcg cgctcgccgg cgccatcgcc
     1081 gccgacccgc agatggccga cgtgctgggc aatccccggt tcacgcgcgc ccagaagctc
     1141 gagctcttct tcggggtggg cggcgacggg ctttcggcgc cggtgaggaa cctcgtgacg
     1201 atcctcgtcg acggcgcccg cgccgtgctg ctgccggaga tctccgccca gttcgacgcg
     1261 ctcaagcgcg agcacgagag cgtgctcaag gtgcgcatcg tgagcgcgag gcccctggcg
     1321 gacgccgagc gcgacgacct ggtggccgag gtcggccgca ggtacaagcg cacggtcgag
     1381 gccacggtgg aaatcgacgc gtccctcatc ggcggcgccc gcgtccacat cggcgacaag
     1441 gtgatccacg cctcgatgcg cgacgcgctc gcccagatgg cagtcgcgct cacgcgctga
     1501 cgaagcacac acgtatctcg gagccacaca tgcaaatcaa tccggctgaa atcagcgaac
     1561 tcatcaagtc gaagatccag aacctccagg tgagcgccga gaagcgcacg gagggaacgg
     1621 tgatctcggt gaccgacggc atctgccgcg tccacggcct cacggacgtg atgcagggcg
     1681 agatgctcga gttcccgggc aacaccttcg gcacggccct gaacctcgag cgcgactccg
     1741 tcggcgccgt ggtgctcggc tcctacgagc acatctccga gggcgacacc gtcaagtgca
     1801 cgggccgcat cctggaggtc ccggtcggcc ccgagctcat cgggcgcgtc gtgaactccc
     1861 tgggccagcc catcgacggc aagggcccga tcgacgccaa gatgaccgag ccgatcgaga
     1921 aggtcgcccc gggcgtcatc tggcgcaaga gcgtgtcgca gccggtgcag acgggcctga
     1981 aggccatcga cgcgatggtg ccggtgggcc gcggccagcg cgagct
//
LOCUS       DASUMX010000172        31286 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_60226, whole genome shotgun sequence.
ACCESSION   DASUMX010000172 DASUMX010000000
VERSION     DASUMX010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 31286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31286
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60226"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..767)
                     /locus_tag="VFZ86_08560"
     CDS             complement(<1..767)
                     /locus_tag="VFZ86_08560"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5642375.1"
                     /translation="MASAKFEREYSQHLVEQVLKGKMTRRQMLVRASVFGLSLTAAGQ
                     LLAACGGDDGGTEASPSASGAPEPVLGGNLKWIGPAPITDPDPVTIYDQGGIVLVYQF
                     LEYLIDLNNDNSLRPKLAESWTPNDAVDVWTFKLRSGVTFNDGSPFGAEDVVTTMERV
                     LDPDNGSGALSQLSGVLSPGGAKAVDDLTVEFNLDKPYADFPYMVSSGNYNTAILSRN
                     YTGDIIKNPVGTGPFMLDQYVTKQKATFKKNPTYWGK"
     gene            1141..1881
                     /locus_tag="VFZ86_08565"
     CDS             1141..1881
                     /locus_tag="VFZ86_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003890470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX5642376.1"
                     /translation="MRVASARRTPERTSPLYLWLAALLRDQIEAGSLSAHGPVPSERV
                     LSERYGVSRMTARHALETLTLEGYLYRNPRRGTFVAEPRLRFSVGSFTRNMAERVPGT
                     EILSAATVAPDPAVAQLLGVPVAGHVHVLQRLRTAMGEPIAIENIQLSAARFPDLLEH
                     DLAGSLWALLRERYGVHPQRADARVVAVILDPFEAEVLGVEAGNPAIVLTRIVYDADG
                     GVVELARDVYRGDRTEFEVSAPVEGVEP"
     gene            complement(1946..3304)
                     /locus_tag="VFZ86_08570"
     CDS             complement(1946..3304)
                     /locus_tag="VFZ86_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008758385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5642377.1"
                     /translation="MRDIAEAARTTPVMHETEVLVVGGGPGGLAAALAAARAGAATTL
                     VDRYGCLGGVITQVGVDTLAWYRHEGTIEAGGIGAEFERRAQEMGATAPDFQSQSQAL
                     DSELFKVVADELVAESGVTPLLHCLVTEPVVEDARVRGVVTESKSGRLAILAERVVDA
                     TGDADLAVRAGAPCHTHPRGSVMGVTVMFSCSGVDKGRFLDHVAASAPTYGDWGKCWS
                     VRTDGKEDGLFSPYLQEPFDRARAEGLIPEDIRSIGGTWGRLTDAGEATSLNMVVMAG
                     YDCTDVRDLTRAEIEGRRQALLAIEALRRHTPGFESARLRAFGSTLGTRDSRKIAGRY
                     ELTGDDVRGEARFEDSVGIFPEFLDGYGVLVLPTTGRYFQVPYGILVPRGLDDLLVAG
                     RCVAGDKISHAATRNQMCCAVTGQAAGVAAAVSLRDGRSTGEVDVTHVQQELRRQGVR
                     LD"
     gene            3424..5175
                     /locus_tag="VFZ86_08575"
     CDS             3424..5175
                     /locus_tag="VFZ86_08575"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HEX5642378.1"
                     /translation="MRKRHAASALTCRSPWDARGRQIERLANELFDVLVVGGGVTGCG
                     VALDAASRGLSVALIEKRDFAAGTSSRSSKLIHGGLRYLERFDLGLVREALHERRLLT
                     QRLAPHLVHPTPFLLPLEHGLKDRAYLGAGLLLYDALAGVRPAMPRHRHLSLEACRRA
                     VPSLKEGAVTGGIRYYDAQVDDARFAVTLAGTASAQGACCVPAVEVTGFLHDGPSLAG
                     VHARDLEDDAELDIRARVTINATGVWTTAMERLAGVASPLPVRPSKGVHIVVPRERID
                     SELALILPTEKSVLFVLPWSTHWIIGTTDTPWEFGLDHPAASRADVDYLLEHANAILR
                     RPLTTEDITAVYVGLRPLVAQDAADTAAVSREHVVRRSAPGLVSIAGGKYTTYRVMAR
                     DAVDVAARDLPFCVPPSHTEAVPLLGAVGSAQAGQRARLHPAAAGLSPGQLDHLARRY
                     GTLALRVLDLVAEDPALGAPVDGAGTYMGAEVRFAVLNEAALHVDDVLTRRTHIAFEA
                     PDRGRRAVEDVARLMAPALEWDEAAVSHEIQHYRERLDAESAAQRMLDDAAADAARAP
                     VRDVRLEHERAGESGAR"
     gene            complement(5172..6260)
                     /locus_tag="VFZ86_08580"
     CDS             complement(5172..6260)
                     /locus_tag="VFZ86_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015904812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX5642379.1"
                     /translation="MTSVDEVIRRQAGRDPSRFEIRPGDQAMLAVVTTGNGGFETLEV
                     REVRRPRPDEGEVLVRVLAAGVNATDVNTRVGWYEAGGWSAPSPFPFIQGTDCCGLAE
                     EVGPGGDATLEGRRVLVRPCMRPHGFSSPDTLWLGSDCDGAFAQYVLAPASEVYPVSA
                     GLSDAELGVVPCSFGTAENMLLRAGVRPGQHVVVCGASGGVGSAAVQLARLRGADVTA
                     LTGSDKAARVLALGASRVVDRAHDPAEVLGEQSADVVVDNVSGPGLDRLLSVLRRGGV
                     YVTSGAVAGPQVSLDKRTLYLRDLTLLGCTAWDEPVFPALVGLLERDELRPPVARVLP
                     LERIAEAQAEFLEKRHVGSFALVPPASS"
     gene            complement(6306..8081)
                     /locus_tag="VFZ86_08585"
     CDS             complement(6306..8081)
                     /locus_tag="VFZ86_08585"
                     /inference="COORDINATES: protein motif:HMM:NF013481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HEX5642380.1"
                     /translation="MDFYTGRILRLDLSRLAATVEPLPEDWARLYVGGKGILLRYLFE
                     ELPPGADALGPENPLILATGPFAGTAAATCSRLAVGCKSPATGTLLDSYVGGSFAPEL
                     KFAGYDLVLATGRAPHPVLVLIEDDRVEFLPAEGRFWGLETAALEQKVRKEMGPWHKV
                     LSTGPAGENLVPFACLSTDQYHKAGRGGAGAVMGSKNLKAVVVRGTGAVTVGDARAFL
                     TDMYALQTERILTADNAWTWEEGTPYLVELVNAAGALPTRNWRSGQFEGAEAIQSAEL
                     QRVRTKIRACTQCPLACRQVHRFGEHVCEGPEFETLGLCGSNCGIGDLATIAAFNHAC
                     DELGLDTMSTGAVVALAMDLCGKGAADYGLTFGDAAGYLAAPGMIARREGWGAELALG
                     ARDLAALKGRPELAPHIKGLELPAYDPRGTFGMGLGYATSDRGACHMRAFTAADDILG
                     GEAPADSLEGKPALTAEQQDFSAVAWTGVWCANMYVDTDFLGAHFRHLWGRETSHEEL
                     MTIGARIWNLGRLLNLREGFAREHDRLPAILSVPHPDGAAAGKTVSAAEFRDALDEYY
                     RVRGWDADGVPTEETLARLSLDVRL"
     gene            8241..8915
                     /locus_tag="VFZ86_08590"
     CDS             8241..8915
                     /locus_tag="VFZ86_08590"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5642381.1"
                     /translation="MTAPPGGWSTDEDGHARLTEHLHFAFEDPLSSLPETARNLLVAA
                     KEIVADEGFEALTLKAVSVRAGENKAMVSYYFDNKAGLVAAVLDSVIHDEYLTSLDRM
                     KDVAPEERIPRLVEEMRRMAESADDFTVFFELLPHVLRDDVLRRRMVMLVRWYWKMKL
                     EWLGIRDGAGGLADPDLLGIAQLLSAIIDGLAIQAAVDPDVDLANPYRALARMLECLE
                     VARADA"
     gene            complement(8948..10351)
                     /locus_tag="VFZ86_08595"
     CDS             complement(8948..10351)
                     /locus_tag="VFZ86_08595"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HEX5642382.1"
                     /translation="MNGAATAVLDERGCRKIHEAALRLLEEVGCAILDPEALALLGAN
                     GARVDGERARFGEALVERARSTAPARYTVAGRRPELDLHVGLGEPAVLASASGPPFVL
                     SGGAYRTGTLADLTTAVRLAHLSPNIDVLGYSVEPGDVPEERRPRVVAHLHATSSDKN
                     ARFTVTSLTELQVATDVLEILYGADWHARPRLWSVINTTSPLQFSAEGAQVLLRLARL
                     GQPVLVAVCAMGGTTAPITPAGLLAVQHAELLVGLVLTQLAAPGTPFLYGGTSSLSSM
                     QSGALMIGSPAYWSLMEATVLLGHWLGVPVRAGGSVTDAHVPDAQAGMESALAMDTVL
                     RTGAQYVLHAAGILSSFNCFSPEKFVIDDEVITGIRTARRPIAVDDETLALDVVAAAG
                     PGGSVLGHAHTRRHARDGIRRTIMNRAPYETWRSQGARDLAAVATARVEELLQSYEPP
                     DDLDTLVRGQLDAYCLG"
     gene            complement(10376..11824)
                     /locus_tag="VFZ86_08600"
     CDS             complement(10376..11824)
                     /locus_tag="VFZ86_08600"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HEX5642383.1"
                     /translation="MTDLPAALESRLKVLSDEKCQMIYDAALTIISEMGMTVPHAEAR
                     EILVKGAGATAEGELVKIPREAVDAARATVPSMIPVYDRNGEPAMELGGYNSYFGTGS
                     DLMSIYDFETRERRLSVLADTARMAHLCDGLPNIDFIMSAAHPHDIEAYASYLECFRA
                     MVSNTTKPMVMTAAGVEDLEVMWKIASAFRGGAEQLREKPYFIQYGEPVSPLQHAPEV
                     LDKLLFCADWGIPLIYSPAPIAGATAPITHAGHIVQAVAESLFGVVIHQLRRPGSPLL
                     TGMGPVKLDMNTVQALYNSAEYYTTYLGITEMAKWMDIPNWGYAGTSDSQCVDANAGI
                     DITELTLLSMQAGSNLNHDIGYLDFGLTGAGELVVLTDEVISMNRRALEGIEVSEETL
                     ALDTIAAVGQGGDFLGKKHTKRHVRNLQWRPTILNRSTQKNWEAAGSLDATEKAHLKL
                     VDIMTNHTPEPLSAEMKQTVDGLVDGYVAAGK"
     gene            complement(11846..13099)
                     /locus_tag="VFZ86_08605"
     CDS             complement(11846..13099)
                     /locus_tag="VFZ86_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012630827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5642384.1"
                     /translation="MHSLAEDLTPPGRGFVPADRVARLGTETVFAVSAEAAALAAQGR
                     TIYPFHLGDLNIPTAEPIVEAMYRAVRDGKTTYCPNAGIMPLREALAEDVGSARGVTY
                     AAENVSVQPGGKPVIGKWLMAAMNMGDEVLYPNPGFPIYSSFIEFVGGTAVPYGYVDD
                     GERYTIDIDAIERRITPRTRLLILNDLHNPTAAECTSEELDRIAEIAVRHDLLVLSDE
                     AYFDVRFRGASESIVSRPGMAERTVILYTFSKKYAMTGWRLGAAVGPAPIIEIITRMN
                     VNIESCPNHFAQYGALEALTGDQTGPREMMARLQGRRDAALRLLNATPGVRCHTPEST
                     FYLYPDVTDAVRLAGCAGHEDFRRLLLDRTGVSFCTRTHFGKALPDEKRYHLRLAYSG
                     VTTAQIEEGLGAFKAFVESHEPAES"
     gene            complement(13126..13980)
                     /gene="panB"
                     /locus_tag="VFZ86_08610"
     CDS             complement(13126..13980)
                     /gene="panB"
                     /locus_tag="VFZ86_08610"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEX5642385.1"
                     /translation="MPAKKGLFDLHEMKRAGEKIAWLTAYDYPTATFEEAAGIDMILV
                     GDSLGMCVYGYPGTVPVTMDQCIVHSEAVRRGAPNTFIVGDMPLGSYQASDEDAVTNA
                     IRFLKEANVDAIKLEGGVRTVSRVKAILDAGVAVCGHIGLTPQSSGQLGGHKAQGRTL
                     ESAKLVVEDARALYEAGVQLLLVEAVPPEVAGFIRRELPIPVLGIGAGEDVDGQLLIV
                     SDVLGTFAAFTPKFVKKYADLAAVSTAALTEYVADVRGSAFPEEQHCYRMLEGEQDKF
                     AAWAADAS"
     gene            complement(14031..14495)
                     /locus_tag="VFZ86_08615"
     CDS             complement(14031..14495)
                     /locus_tag="VFZ86_08615"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX5642386.1"
                     /translation="MDALDREVLLALQEDGRMSNLALARKLGLSPSAMLGRVRRLERS
                     GAIRGYRAVIDPAALGVTLRALVVARLREHSEESIREFENGIKEVPGVRACYHVTGQF
                     DMVVELALRDLDHLGQLIRVDIARIPGVMNLETMLVLAESVADEGWPIVVEE"
     gene            complement(14703..15695)
                     /locus_tag="VFZ86_08620"
     CDS             complement(14703..15695)
                     /locus_tag="VFZ86_08620"
                     /inference="COORDINATES: protein motif:HMM:NF012968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX5642387.1"
                     /translation="MAGETPRIALVLDATGAAAERHAQALGGFLRDGLRFSPEHPADA
                     VTHLFYAEESARERLLALAPTRLVRLVRAPPHRPDLMAAALAAFAAGGGAELFVFGGP
                     LGAESAARLAARSGGGVLTGVLDAVAAAGALSCRRHVSSHHLIGRFELRARPWCVTLD
                     AGWLDAGFEPPAEHVVQASTELREDDSSAPRLCDDVEVFEPPATSDLEAAQFLVVAGR
                     GAGGRAGVERIATAADRMGAAFGVTRPVVMNAWAAPDRQIGVSGTRAAPAVCIVAGSS
                     GAPAFLWGVERAGFIVAVDTDEHSAMAGEADAVVLDDAVTVLEALADIVARERL"
     gene            complement(15695..16528)
                     /locus_tag="VFZ86_08625"
     CDS             complement(15695..16528)
                     /locus_tag="VFZ86_08625"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642388.1"
                     /translation="MTALRIAVGFKVTPDYEALRPADWARAATGPSAHGEAVRYVRRV
                     FNVFDEAALELALRLRDARTERGLETSLVAFSVAGREADPFLATLQALGYEGARVEPD
                     ADLDFAPATTAALVAAATRHLGGEVLLLGSRCGPGDTGLVPFLAAEALGRPSLPGVTA
                     VEPAADRLRVTFSAGDQPLRATIAPPCVLAVGNALVSLLRVPTLKDRLATRDRVVTTL
                     TPADLGVDVPAALSREPSALVGLETIDRSRAGVVVAGASPAEEARALYEEHLHARLER
                     I"
     gene            complement(16554..17996)
                     /locus_tag="VFZ86_08630"
     CDS             complement(16554..17996)
                     /locus_tag="VFZ86_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX5642389.1"
                     /translation="MPLSREAIVEGLRGIVGPDAVVTDEKVLVESSVDNFRKLQNIFD
                     VHTMPVPAAIALPADTAQVARVLTWADDNGVNIIPRTGATATEGGLESVVPDSVILDG
                     SAMNEIVKVDPYNMQATCQCGVPLQALEDRAREHGLTTGHSPQSKPVAHMGGLVATRS
                     IGQFSTLYGGIEDMVVGLEAVFPGGEVCRIKNVPRRAAGPDIRHIVIGNEGALCFITE
                     VTVKLFPYFPENNVFLGWTLKDMGLGFEVLRDVMVNGYKPSVARVYDPGDAGIHFSHF
                     AAPDDCVLLFMAEGAAGIATATADGIQAIVDQHPECTQVPSEYIETWFNDLCWDASKI
                     TAEDERIRQTRNVNRTTEISADWSSINEIYQAAKQRIIDEIPDITMMGGHSSHSYING
                     TNMYFNYFFNVVGVGPEEETEKYYLPIIGIICEETLKRGGSIVHHHGMGKARAPWVKD
                     EYGSSYSMLETLKRSFDPNGVMNKGTIIPL"
     gene            complement(18034..19464)
                     /locus_tag="VFZ86_08635"
     CDS             complement(18034..19464)
                     /locus_tag="VFZ86_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006444265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HEX5642390.1"
                     /translation="MAGKYLIGIDNGTQSTKVVIYDLEGTIVCEARRTLLPMEHPQPG
                     YAVHPDDDLWESIVAASREVMTKFTGDPTDILGVGLCTIRCCKTFLKEDGSLLEPVMS
                     WMDTRAYQPWIPDDPELAYATTSSGYMTHRFTGQFRDSAANNIILQWPIDTDTWQWDP
                     ALFERFNVTRDLLVELQMPGEVAGYVTAEAAAATGIPEGLPVVTTANDKAVEALGSGS
                     LDERTALVSLGTYIASMVHGHENRQTAVEFWTNFACIPHRYLYESGGVRRGMWTLSWF
                     LDLLGPELAAAAATQEVSREEYLEREAELVPAGSCGLMTVLDWLAPTDKPFRKGEIIG
                     FDARHTRAHVYRSILEAIALTMKQNVDAMCAELGIDLREIVVSGGGSNSPLFMNIFAD
                     VFGVPASRNVGSSGASLGSAICAAVATGAYPDFPTAAGAMKQSRESFAPNLENHDVYR
                     RMNDKAYRAIHEATDPILEQSFSIFH"
     gene            complement(19483..20238)
                     /locus_tag="VFZ86_08640"
     CDS             complement(19483..20238)
                     /locus_tag="VFZ86_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5642391.1"
                     /translation="MDYFSLKGKNAIVTGGNTGLGQAFTLALAAAGADLFVPNIIDDD
                     GQTGPLVEERGGRYEFMKADITAAGAPAEIVAACVDRLGSLDILVNSAGICPLAAVLD
                     FGREQWDATVAVNLTAAFEMAYEAAKVMIPQRSGKIINICSLFSFLGGRWSSAYAATK
                     HGLAGFTKAYCDELAEYDIQVNGIAPGYYATEITTATRSDPVTNQRVLDHIPAARWGD
                     PADLMGATVFLAGRASDYVSGHVLVVDGGYLVR"
     gene            complement(20385..21305)
                     /locus_tag="VFZ86_08645"
     CDS             complement(20385..21305)
                     /locus_tag="VFZ86_08645"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5642392.1"
                     /translation="MTLKIGYIGLGALGRHLAGSLLRAGFPLTVYDVDASCSVDLGKA
                     GATCAGSIAETAKASDTIITCLPSPEAVASVVAEEGGVLENLAPGGTWIDMSTNDAHE
                     LQRLSSLAAAKGVATLEAPVTGGVHRAAAGMITVLVGGDEEVYEKHRPAFDAMGGRVF
                     YIGELGQASVIKVITNMLAFIHLVADGEALMLAKQGGIDLKLAWDVIKASSGNSFVHE
                     TEGQLILSGSYNINFTMDLARKDLFFAHQMGREFGVPLELGGLTEQIFARARAMYGGG
                     AQSPMVVKMLEEAVGTELRAPGFPTELEPE"
     gene            complement(21349..22881)
                     /locus_tag="VFZ86_08650"
     CDS             complement(21349..22881)
                     /locus_tag="VFZ86_08650"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HEX5642393.1"
                     /translation="MPRKRAARRRPPHSTRPQGAHTDGEGSMRARTQWLSEAEKSAIV
                     DEAVELLDRVGMRYAGSAVLPLLEERGAQVDHATGIARLPRQLVEWAAGQCPRSVLMA
                     GLTEADDVLLDEGEPFHFVPSGCVAKTLDFRTGRRRASTLQDLRECTALMDELPQLDI
                     MWTQVSASDVPLEQRELTEYFTMFTETSKHVTFVDCPGEVDAVLRICEALSGDLDRFR
                     ARPRISTVVTAASPLQVDGGALDTHIALARHGVPIEVYSMTIAGATSPVTLAGSVTQG
                     LAEFLGIATALQVSAPGARLVFCFGSGVLDMLRTTFALGSLESGLMGAMATEVGHYLG
                     VPTLNPGLSTDAKHAGIQAGYEKAMKAATVCGAVPDMVSGWGLIDSHNTMSLPQSVID
                     NEMAAMLHRLHRPVEVSEATRAGEAIAAVGPGGGFLGVKETAKRIRAGEHYMPTLSNR
                     LSYEKWAEDGVKEFDDACAQVELRLAEHASKEPYIDGAQLDEIAAICRADDEALRRAR
                     RS"
     gene            23058..23552
                     /locus_tag="VFZ86_08655"
     CDS             23058..23552
                     /locus_tag="VFZ86_08655"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX5642394.1"
                     /translation="MGTTELDDLDRRIVKLLRPNARRSYASIARVIGVSEPTVRNRVD
                     RLIKREAILPWTGVNPAAIGLPVDVMVGIRVNRGYGKGVGESLAAMKNVAYLGYTTGS
                     FDILIEVHLPDNEALYRFLNEDLDQIEGIAYTDTWHILRTDTTNFEWAGEDAGNEPLG
                     DEAP"
     gene            23746..25110
                     /gene="mtbB"
                     /locus_tag="VFZ86_08660"
                     /pseudo
     CDS             23746..25110
                     /gene="mtbB"
                     /locus_tag="VFZ86_08660"
                     /inference="COORDINATES: protein motif:HMM:NF021053.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="[dimethylamine--corrinoid protein]
                     Co-methyltransferase"
     gene            25113..26582
                     /locus_tag="VFZ86_08665"
                     /pseudo
     CDS             25113..26582
                     /locus_tag="VFZ86_08665"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
     gene            26668..28104
                     /locus_tag="VFZ86_08670"
     CDS             26668..28104
                     /locus_tag="VFZ86_08670"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HEX5642395.1"
                     /translation="MSYSSKEIGAGRVSFLTEEQKQRIYATALEILGEMGMVVLHDEG
                     EQVMLEGGCTKDADGNVRVPAALVEQARESVPAGFLVYDRAGEPAMDLGGLRSYFGNG
                     SDVMHLHDLETGERRLGRLADVTEAARLCDALPDIDFVMSGAYPDGMDASEAYPRQFR
                     AMIAATAKPLVMTAGGVETVEPMWKMACAVRGGAEALRRKPYFVMYNQPVSPLKHPFE
                     TVDKLLFCADRGIPSTYCPSPVAGGTAPITTAGQITLGIAEALFGLVMHQLRAPGAPF
                     VIGQGPNTLDMATSQSSYNSPEFIRAYACEVEMAKWLDIPNWGFSGHTDAQVVDAQAG
                     MEADEITKLSMQLGANLNHDVGYMDFGLSGSLAEVVLVAEFISRNRQLLKEIEVSDET
                     LAVDVIKAVGPGGDFLGQRHTRRHLRGNQWRGQLLNRASYDRWMENGGSDVTEKARQK
                     ARDLLATHEVAPLPEGLAERLDAVVPAG"
     gene            complement(28135..29625)
                     /locus_tag="VFZ86_08675"
     CDS             complement(28135..29625)
                     /locus_tag="VFZ86_08675"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5642396.1"
                     /translation="MSQDPLHDFQLLIRSRYGALLVETTEEDRAEALAERAAWQLQLP
                     LFVWRRATGLRRAGAPTGVYGTEDPRTALGHVVSSQLPALYVFHGLGPELERPDVADL
                     LREAARQLAGRSGAIVLTGANVRLPGSLRVEVGPLHLPMPTRDEYESLQQRVVKDVST
                     RMGVQVSLAPADQDRLLANLQGLTLAEAEKLLTRVLVEDGRLDAGDVKRIVEAKRELV
                     ERDGLLEFQPAVHSLDDIADLVHLKEWLEKRRLFMEDRQRAADFGLEFPRGVLLVGVP
                     GCGKSLCAKAVAAAWGLPLVRLDPGALYDKYIGETEQNFRRAMASADRLAPLVLWIDE
                     IEKAFAAGGEDADGGTSLRVLGSFLSWLQERRGDVFVVATANDVQRLPAELLRKGRFD
                     ETFFVDLPDEETRAVILDIHLRRRKQAPDRFDLRLLAAESEGYSGAELEQVVISALYT
                     AFSAKVAPTTALLLDELRRTPPIASTAREKIAFLRAWAEGRTVPAN"
     gene            complement(29622..30608)
                     /locus_tag="VFZ86_08680"
     CDS             complement(29622..30608)
                     /locus_tag="VFZ86_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642397.1"
                     /translation="MGDSDAVADSSAVPAEVIPGLRTLAERVGAPDFVLPWLDRFYDQ
                     GDVDVVLAAGDGDARRADDGLARFGVTQLDRAVRRAVLDRDEAGVQRPADFHARLEIW
                     AMFEGWKDVPLAVHRRLADWEVDSYATRIRPDVEATRDGRPGDSYEARYAYVLLEEAE
                     TIIEAQDHIYLWPCDCRSIVSRCRKPMNVCLRFENDRGLGWEISRERARDVLRTADKA
                     GLMHTADVHDDPAGTSSICNCCTDCCFPHLAADRLGVSAAWPVRRHIARIDMNHCKAC
                     GRCGLRCPFAAIVCRSDGRPGFDAALCRGCGLCATACSADAIALLALAEGRR"
     gene            complement(30776..31186)
                     /locus_tag="VFZ86_08685"
     CDS             complement(30776..31186)
                     /locus_tag="VFZ86_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019341644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEX5642398.1"
                     /translation="MPRVRSSILLVCGWTSLGLGVVGLAVPILPTVPFVLLAAACFVR
                     SSERCHRWLVTHPLFGRHIADYEAGRGIARRAKIVALATLWASVLLSVFLFVPLLAAD
                     VAMLAVAAAVTIYIVRLPTADARGAAPQDPTHDG"
BASE COUNT         4604 a        10696 c        11282 g         4704 t
ORIGIN      
        1 tccttgcccc agtaggtggg gttcttcttg aaggtggcct tctgcttggt cacgtactgg
       61 tcgagcatga acgggccggt gccgaccggg ttcttgatga tgtcgcccgt gtagttgcgc
      121 gacaggatgg cggtgttgta gttgccggag gacaccatgt acgggaagtc ggcgtacggc
      181 ttgtcgaggt tgaactcgac ggtgaggtcg tcgacggcct tggcgccgcc gggcgagagg
      241 acgccgctga gctgcgagag ggcgccggag ccgttgtcgg gatcaaggac gcgctccatg
      301 gtggtgacga cgtcctcggc gccgaagggg ctgccgtcgt tgaacgtgac gccggagcga
      361 agcttgaagg tccagacgtc gaccgcgtcg ttcggcgtcc agctctcggc gagcttgggc
      421 cgcagcgagt tgtcgttgtt gaggtcgatg aggtactcga ggaactggta gacgaggacg
      481 atgccgccct ggtcgtagat ggtgaccggg tcgggatcgg tgatcggcgc ggggccgatc
      541 cacttgaggt tgccgcccag gacgggctcg ggcgcgccgg acgcgctggg tgatgcctcg
      601 gtgcccccgt cgtcgccgcc gcaggcggcc aggagctggc cggcggccgt cagcgacagg
      661 ccgaacaccg acgcacgcac gagcatctga cgcctggtca tcttgccttt gaggacctgc
      721 tcgaccaggt gctgactgta ctctctctcg aacttcgcgc tagccatatc gttactcccc
      781 ctgacgcagt cttgtgtgaa ccacacagaa tctggcgata gcccgctgca gggccgcccc
      841 cgatagatag gggtcaccat acacacgctc gctgtggata gcaaggtgga attgaccaat
      901 cagttagtcg ccgtccccct ctatcaagtg cagatattcg agtcaaagca tgactgaccg
      961 gatttcaggt ccttcggccg tgctttgacc gatagaaatg cgccggaccc ccgcagccgc
     1021 cgtcggtcgg agcgacgctc ggatgccttg gtatgtacca tatccccgca tggtacgctt
     1081 gccgcgggcc ggccgcagcc gagacgccgg ccgcgactga ggatctcgag ctgccagggg
     1141 gtgagggtcg cgagcgcacg gcgcacaccg gaacggacga gcccgctgta tctgtggctc
     1201 gccgcgctcc tccgcgacca gatcgaggcc gggagcctca gcgcgcacgg ccccgtcccc
     1261 tccgagcgcg tcctgagcga acggtacggc gtcagccgca tgaccgctcg tcacgccctc
     1321 gagacactga ccctcgaggg ctatctctac aggaacccca ggcgcggcac cttcgtggcg
     1381 gagccgcgcc tgcgcttcag cgtcggcagc ttcacgcgca acatggccga acgagtgccg
     1441 ggcaccgaga tcctctcggc ggcgaccgtg gcgccggacc cggcggtcgc tcaactgctg
     1501 ggtgtccccg tcgccggcca cgtgcacgtg ctgcagcgcc tgcgcaccgc gatgggagag
     1561 ccgatcgcca tcgagaacat ccagctctcg gccgcgcgct tccccgacct gctggagcac
     1621 gacctggccg gatccctctg ggcgctcctg cgcgagcgtt acggcgtgca tccccagcgg
     1681 gcggacgcgc gcgtggtcgc cgtcatcctg gacccgttcg aggccgaggt gctgggcgtc
     1741 gaagcgggga atcccgccat cgtcctcacg cgcatcgtgt acgacgcgga cggcggcgtg
     1801 gtggagctgg cgcgggatgt ctaccgcggc gaccgcacgg agttcgaggt gagcgcgccc
     1861 gtggagggcg tcgagcccta gcgccggccg ggcggggcgg tgaggccgcc ctgcggatgg
     1921 gccggacggt gagctgcgcc ggcggtcagt ccagccgcac gccctggcgc cgcagctcct
     1981 gctgtacgtg cgtcacgtcg acctcgcccg tcgagcggcc gtcacggaga gagacggccg
     2041 ccgcgacccc ggcagcctgc cccgtgacgg cgcagcacat ctggttgcgc gtcgccgcgt
     2101 gcgagatctt gtcaccggct acgcagcggc cggcgacgag caggtcgtcc aggccccgcg
     2161 ggacgaggat gccgtagggc acctggaagt agcgcccggt ggtcggcagc accagcaccc
     2221 cgtagccgtc gaggaactcg gggaagatcc ccaccgagtc ctcgaagcgc gcctcgccgc
     2281 gcacgtcgtc gccggtcagc tcgtagcggc cggcgatctt gcgagagtcc cgcgtcccca
     2341 gcgtcgagcc gaacgcccgc aggcgcgcgc tctcgaagcc gggcgtgtgg cgccggagag
     2401 cctcgatggc cagtagcgcc tggcggcgcc cctcgatctc ggccctcgtg aggtcgcgca
     2461 cgtccgtgca gtcgtagccc gccatcacga ccatgttcag cgaggtcgcc tctccggcat
     2521 cggtgaggcg gccccacgtc cctccgatgc tgcggatgtc ctcggggatg aggccttcgg
     2581 cacgggcccg gtcgaacggc tcctggaggt aggggctgaa gagaccatcc tcttttccgt
     2641 ccgtcctgac gctccagcac ttgccccagt cgccgtaggt cggcgcgctc gccgcgacgt
     2701 ggtcgaggaa gcgccccttg tcgacgccgg agcacgagaa catcacggtg acgcccatga
     2761 cggagccgcg cggatgggtg tggcaggggg cgccggcgcg gacggcgagg tccgcgtcgc
     2821 cggtcgcgtc cacgacgcgt tccgcgagaa tggcgaggcg gccggacttg ctctcggtca
     2881 cgacgcctct gacgcgggcg tcctcgacga ccggctcggt gacgaggcag tggagcagcg
     2941 gcgtcacgcc ggactcagcc acgagctcgt ccgcaaccac cttgaagagc tcggagtcga
     3001 gcgcctggct ctgcgattgg aagtccggcg ccgtagcgcc catctcctgg gcgcggcgct
     3061 cgaactcggc gccgatgccg ccggcctcga tggtgccctc gtgccggtac caggccaagg
     3121 tgtcgacgcc gacctgggtg atgacgccgc cgagacaccc gtagcggtcg accagggtgg
     3181 tcgccgctcc ggcgcgagca gcggcaaggg cggcggcgag accgcccggc ccgccgccca
     3241 ccacgagaac ctccgtctcg tgcatgaccg gcgtcgtcct cgccgcctcc gcgatgtccc
     3301 tcatcgagct ctcccgtggc gcgaggcgcc gtcgtcccgt cactgtcttg acagagtatg
     3361 gcacagggtt catggtacat accagacggc gtggtacata ccaagagcga ccgaggagcg
     3421 acgatgagga aacgccacgc ggcatccgcc ttgacctgcc gttcgccgtg ggacgcccgt
     3481 gggcggcaga tcgaacggct ggcgaacgag ctcttcgacg tgctcgtcgt cggcggcggc
     3541 gtcacgggct gcggagtagc gctcgatgcc gccagtcgcg ggctctcggt cgccctgatc
     3601 gagaagcgcg acttcgccgc cggcacatcc agccggtcca gcaagctcat ccacggcggc
     3661 ctgcggtacc tggagcgctt cgacctcggg ctcgtgcgcg aggcgctgca cgaacggcgc
     3721 ctgctcaccc agcgcctcgc gccgcatctc gtccacccga cgccgttcct gctcccgctc
     3781 gagcacggtc tcaaggatcg cgcctatctg ggcgcgggcc tgctgctgta cgacgccttg
     3841 gccggcgtgc gccctgcgat gccgcggcac cggcacctct cgctcgaggc ctgtcggcgc
     3901 gcggtgccgt cgctcaagga gggcgccgtc accggaggta tccgctacta cgacgcgcag
     3961 gtggacgacg cccgcttcgc ggtcacgctg gccggcaccg cgtccgcgca gggtgcctgc
     4021 tgcgtgccgg ccgtcgaggt gacggggttc cttcatgatg gcccatctct ggcgggtgtc
     4081 cacgcacgcg acctggagga tgacgcggag ctcgacatcc gcgcccgtgt gaccatcaac
     4141 gcgaccggcg tctggaccac ggcgatggag cgcctcgccg gcgtcgcgtc cccgctgccg
     4201 gtgcgcccgt cgaaaggggt ccacatcgtc gtgccacgcg agcgcatcga ctcggagctc
     4261 gccctcatcc tccccaccga gaagagcgtg ctcttcgtgc tcccgtggag cacgcactgg
     4321 atcatcggca cgaccgacac gccgtgggag ttcggcctgg accaccccgc cgccagccgc
     4381 gccgacgtcg actacctgct ggagcacgcc aacgcgatcc tgcgccggcc gctgaccacc
     4441 gaggacatca cggccgtgta cgtcgggctc cggccgctgg tcgcgcagga tgcggcggat
     4501 accgccgccg tctcgcgcga gcacgtcgtg cggcgcagcg caccggggct cgtgagcatc
     4561 gccggcggca agtacacgac ctaccgcgtc atggcgcgcg acgccgtcga cgtcgctgcg
     4621 cgggaccttc ccttctgcgt gccgccgagc cacacggagg ccgtgccgct gctcggcgcc
     4681 gtcggatcgg cgcaggcggg gcagcgggcg cggctccatc cggcggcggc cggcctctcg
     4741 cccgggcagc tcgaccacct tgcgcgccgg tacggcacgc ttgcgctccg ggtcctcgac
     4801 ctggtcgccg aggacccggc gctcggcgca cccgtggacg gcgccggcac ctacatgggc
     4861 gcggaggtgc gcttcgccgt gctcaacgag gcggcgctgc acgtcgacga cgtgctcacg
     4921 cgccgcacgc acatcgcctt cgaagcgccc gaccgcggcc gccgcgccgt cgaggacgtc
     4981 gcgcgcctga tggctccggc gctcgagtgg gacgaggcgg cggtcagcca cgagattcag
     5041 cactaccgcg aacggctcga cgccgagagc gccgcgcaga ggatgctgga cgacgcggcc
     5101 gcggacgcgg cgcgcgcgcc ggtgcgcgac gtacgcctgg agcacgagcg cgcaggcgag
     5161 agcggagcgc gctagctgga cgcgggaggc acgagggcga agctgcccac gtgccgcttc
     5221 tcgaggaact ccgcctgcgc ttcggcgatg cgctcgagcg ggaggacgcg agccaccggc
     5281 ggccgcagtt cgtcacgctc aaggaggccg acgagcgccg ggaagaccgg ctcgtcccag
     5341 gcggtgcatc cgagcagggt gaggtcgcgc aggtagagcg tgcgcttgtc gaggctcacc
     5401 tgcgggccgg cgacggcgcc cgaggtgacg tagacgccgc cccggcgcag tacgctcagc
     5461 aggcggtcga gtcccggccc ggagacgttg tcgacgacca cgtcggcgga ctgctccccg
     5521 aggacctcag ccgggtcgtg cgctcggtcc acgacgcggc tcgcaccgag cgccagcacc
     5581 cgtgcggcct tgtcgctacc ggtgagcgcc gtcacgtccg cgccacgcag gcgcgcgagc
     5641 tgcacggcgg ccgatccaac cccgccggag gcgccgcata cgacgacgtg ctgcccggga
     5701 cgcacgccgg cgcggagcag catgttctcg gcggtcccga aggagcacgg gaccacgccg
     5761 agctcggcgt cgctgaggcc ggcgctgacc gggtacacct cggacgccgg cgcgaggaca
     5821 tactgcgcga acgcgccgtc gcagtccgag cccagccaga gcgtgtcggg tgaggagaag
     5881 ccgtgcgggc gcatgcaggg cctgaccagc acgcgcctgc cctccagtgt cgcgtcgcca
     5941 cccggcccta cctcctccgc gagaccgcag cagtcggtgc cctggatgaa ggggaacggt
     6001 gaaggcgcgc tccagccgcc ggcctcgtac cagccgacgc gggtgttgac gtcggtggcg
     6061 ttgacgcccg cggcgagtac ccggacgagc acctcgccct cgtccggacg cggccgccgc
     6121 acctcgcgca cctccagcgt ctcgaatccg ccgttcccgg tggtgacgac ggcgagcatc
     6181 gcctggtcgc cggggcggat ctcgaaccgg ctggggtccc gcccggcctg gcggcggatc
     6241 acctcgtcca cggacgtcac ggcgccccct tcgcggcctg ccgggccggc tgcggccgaa
     6301 ggccgctaga gccggacgtc cagcgagagc ctcgccagcg tctcttcggt gggcacgccg
     6361 tcggcgtccc accctcgcac gcggtagtac tcgtccagcg cgtcccggaa ctcagccgcg
     6421 ctcacggtct tgcccgcggc ggcgccgtcg ggatgcggca cggagaggat cgccggcagc
     6481 cggtcgtgct cgcgcgcgaa cccttcgcgc agattgagca gcctgccgag gttccagata
     6541 cgcgcgccga tggtcatgag ctcctcgtgg gacgtctcgc gcccccagag atggcggaag
     6601 tgggcgccga ggaagtcggt gtcgacgtac atgttggcgc accagacgcc ggtccaggcc
     6661 acggcgctga agtcctgctg ctccgcggtg agcgccggct tgccctcgag ggagtcggcc
     6721 ggcgcttctc ctccgaggat gtcgtccgcc gcggtgaagg cacgcatgtg gcaggcgccg
     6781 cggtcggagg tcgcgtagcc caggcccatg ccgaaggtgc cgcgggggtc gtaggccggc
     6841 agctcgagac ccttgatgtg cggggcgagc tcgggccggc ccttgagcgc ggcgaggtcg
     6901 cgggcgccga gcgccagctc ggcgccccag ccctctcgcc gcgcgatcat ccccggcgcc
     6961 gcgaggtagc cggccgcgtc gccgaaggtg aggccgtagt cggcggcgcc tttgccgcac
     7021 aggtccatcg cgagggccac gacggccccc gtcgacatcg tgtccagccc cagctcgtcg
     7081 caggcgtggt tgaaggcggc gatggtggcg agatcgccga tgccgcagtt ggagccgcac
     7141 aggccgaggg tctcgaactc cggcccctcg cagacgtgct ccccgaaccg gtgcacctgc
     7201 cggcaggcga gcgggcactg cgtgcaggcg cgtatcttgg tgcgcacgcg ctgcaactcg
     7261 gcggactgga tggcctcggc tccctcgaac tggcccgagc gccagttgcg cgtcggcagg
     7321 gcccccgccg cgttcaccag ctcgacgaga tacggtgtgc cctcctccca ggtccaggcg
     7381 ttgtcggcgg tgaggatccg ctcggtctgg agggcgtaca tgtccgtgag gaaggcgcgc
     7441 gcgtcgccga cggtgaccgc gccggtgccg cggaccacga cggccttgag gttcttgctg
     7501 cccatcacgg cgcctgcgcc gccgcgcccg gccttgtggt actggtcggt ggagagacag
     7561 gcgaagggga ccaggttctc gccggcgggg cccgtggaga ggaccttgtg ccacgggccc
     7621 atctccttgc ggaccttctg ctcgagggcc gccgtctcga ggccccagaa gcggccctcg
     7681 gccggcagga actccacccg gtcgtcctcg atcagtacga gcaccgggtg cggcgccctg
     7741 ccggtcgcca gcacgaggtc gtacccggcg aacttgagtt cgggagcgaa cgacccgccg
     7801 acgtaggagt cgaggagggt cccggttgcc gggctcttac agccaacggc caggcgcgag
     7861 cacgtggccg ccgccgtacc tgcgaagggg ccggtggcga ggatgagggg attctccggg
     7921 ccaagggcgt cggcgccggg agggagctcc tcgaagaggt agcgcaagag gatgcccttg
     7981 ccgccgacgt acagccgcgc ccagtcctcg gggagaggct cgaccgtcgc cgcaaggcga
     8041 ctgaggtcga gccgcaggat ccgtccggtg tagaagtcca cgccgctcct cgccacaggc
     8101 ccgccatggt cgtccaggag gacccgtgct acaatggcgc ccattaatcg accggtcaat
     8161 tagccgcttc attgcaccgt gaccggaggg tgatgtcaac cccggtccgc gtcgccgggc
     8221 cactgggagg cgaacgtccg atgacggcac cgccgggagg ctggagcacc gacgaggacg
     8281 gtcacgcccg cctcacggag catctccact tcgccttcga agaccccctg tcgtcgctgc
     8341 cggagacggc gcgcaacctg ctcgtcgcag ccaaggagat cgtcgccgac gaggggttcg
     8401 aggctctgac gctgaaagcc gtcagcgtgc gcgcggggga gaacaaggcg atggtctcct
     8461 actacttcga caacaaggcc ggtctcgtcg ccgccgtgct cgactccgtg atccacgacg
     8521 agtacctcac ctcactcgac cgcatgaagg acgtcgcccc ggaagagcgc atccccaggc
     8581 tggtcgagga gatgcggcgc atggcggagt cggccgacga cttcacggtg ttcttcgagc
     8641 tcctgccgca cgtgctgcgc gacgacgtgc tgcgtcgccg catggtcatg ctggtccgct
     8701 ggtactggaa gatgaagctc gagtggctgg gaatccggga cggcgcagga ggccttgccg
     8761 accccgacct gctcggcatc gcccagctcc tgagcgccat catcgacggc ctcgcgatcc
     8821 aggcggccgt cgacccggac gtcgacctcg ccaatccgta ccgtgcgctt gcgcggatgc
     8881 tcgagtgcct cgaggtggcc agggccgacg cctgagcagg ccggcgcggc ggaggctccg
     8941 ccgcgcctca gcccaggcag taggcgtcga gctgcccgcg gacgagcgtg tccaggtcgt
     9001 ccggcggctc gtaggactgc aggagctcct cgacacgtgc cgtcgcgacg gcggcgaggt
     9061 cccgcgcgcc ttgggaccgc caggtctcgt agggcgcgcg gttcatgatc gtccgccgga
     9121 tgccgtcgcg cgcgtgccgg cgggtgtgcg cgtggccgag cacggagccg cccggccccg
     9181 ccgccgccac gacgtccagc gccagggtct cgtcgtcgac cgcgatgggc cgccgcgcgg
     9241 tgcggatgcc cgtgatgacc tcgtcgtcga tgacgaactt ctccggcgag aagcagttga
     9301 aggacgagag gatgccggcg gcgtgcagga cgtactgcgc gccggtgcgc agtacggtgt
     9361 ccatggccag cgccgactcc atgccggcct gggcgtcggg cacgtgcgcg tcggtgacgg
     9421 agccgccggc gcgcacgggg acgccgagcc agtggccgag gaggaccgtg gcctccatga
     9481 gcgaccagta cgccggcgag ccgatcatga gcgcaccgct ctgcatggag gagagcgacg
     9541 acgtgccgcc gtacaggaac ggcgtgcccg gggccgcgag ttgggtgagc acgaggccga
     9601 cgagcagctc ggcgtgctgc acggccagca ggccggccgg cgtgatcgga gcggtcgtgc
     9661 cgcccatggc gcagacggcc acgagcacgg gctggccgag acgcgccaga cgcagcagca
     9721 cctgcgcgcc ctccgccgag aactgcagcg gcgacgtcgt gttgatgacc gaccagaggc
     9781 gcggccgcgc gtgccagtcg gcgccgtaca ggatctcgag gacgtccgtg gccacctgga
     9841 gctcggtgag tgaggtgacc gtgaagcggg cgttcttgtc gctcgacgtg gcgtgcagat
     9901 gcgccacgac tctgggccgc cgctcctccg gcacgtcgcc cggctctacg ctgtaaccga
     9961 ggacgtcgat gttgggcgag aggtgggcca gccgcacagc cgtcgtcagg tcggcgagcg
    10021 tgcccgtgcg gtacgcgccg cccgagagga cgaagggcgg gccggaggcg ctcgccagca
    10081 cggcgggctc gccgagcccg acgtgcaggt cgagctcggg ccgccggccc gccaccgtgt
    10141 agcgcgcggg ggcggtggag cgcgcccgct cgacgagtgc ttcgccgaag cgggcgcgct
    10201 ccccgtccac ccgagcgccg ttcgccccca ggagcgcgag cgcctcaggg tcgaggatgg
    10261 cgcagccgac ctcctcgagc agccggaggg ctgcctcgtg gatcttgcgg cagccccgtt
    10321 cgtcgaggac ggccgtggcg gctccgttca ctccggctcc tgcatgcccg tcctgctact
    10381 tgccggccgc gacgtacccg tcgaccagtc cgtccaccgt ctgcttcatc tcggcggaga
    10441 gcggctccgg cgtgtggttg gtcatgatgt cgacgagctt gaggtgcgcc ttctcggtgg
    10501 cgtcgagcga gccggcggcc tcccagttct tctgcgtgct gcgattgagg atcgtcggcc
    10561 gccactgcag gttccgcacg tgccgcttgg tgtgcttctt gccgaggaag tcgccgccct
    10621 gaccgacggc tgcgatggtg tcgagcgcca gcgtctcctc gcttacctcg atgccctcga
    10681 gcgcgcgccg gttcatggag atgacctcgt cggtgagcac gaccagctcg cccgcgccgg
    10741 tgaggccgaa gtcgaggtag ccgatgtcgt ggttgaggtt cgagccggcc tgcatggaga
    10801 gcagcgtgag ctcggtgatg tcgatgcccg cgttggcgtc gacgcactgc gagtcgctgg
    10861 tgccggcgta gccccagttg gggatgtcca tccacttggc catctcggtg atgccgaggt
    10921 aggtggtgta gtactcggcc gagttgtaga gcgcctggac ggtgttcata tccagcttga
    10981 ccgggcccat gccggtgagc agcggagagc cgggacgacg cagctggtgg atgaccacgc
    11041 cgaagagcga ctcggcgacg gcctgcacga tgtggccggc gtgggtgatc ggggccgtgg
    11101 cgccggcgat cggagccggc gagtagatga gggggatgcc ccagtcggcg cagaagagca
    11161 gcttgtcgag cacctcgggg gcgtgctgca gcgggctgac gggctcgccg tactggatga
    11221 agtacggctt ctcgcgcagc tgctcggcgc cgccgcggaa ggccgaggcg atcttccaca
    11281 tgacctcgag gtcctcgacc ccggcggcgg tcatgaccat cggcttggtc gtgttgctga
    11341 ccatggcgcg gaagcactcg aggtaggagg cgtaggcctc gatgtcgtgc gggtgcgcgg
    11401 ccgacatgat gaagtcgatg ttggggaggc cgtcgcacag gtgcgccatg cgcgccgtgt
    11461 cggcgaggac gctcagacgg cgctcgcgtg tctcgaagtc gtagatgctc atgaggtccg
    11521 agcccgtacc gaagtacgag ttgtagccgc cgagctccat ggcgggctcg ccgttgcggt
    11581 cgtacacggg gatcatcgac ggcacggtgg cgcgggcggc gtccacggct tcgcgcggga
    11641 tcttgaccag ctcgccctcg gcggtggcgc cggcgccctt gacgaggatc tcgcgcgcct
    11701 cggcgtgggg gacggtcatg cccatctccg agatgatcgt gagggcggcg tcgtagatca
    11761 tctggcactt ctcgtccgag agcaccttga ggcggctctc gagggcggcg ggcaggtcgg
    11821 tcatcgtggt gtctccttgg ggggtctagg actctgcggg ctcatgggac tcgacaaagg
    11881 ccttgaacgc gccgaggccc tcctcgatct gtgcggtggt gacgcctgaa taggccaggc
    11941 gcagatggta ccgcttctca tccggcagcg ccttcccgaa gtgcgtgcgg gtgcagaacg
    12001 acacgccggt ccggtcgagg agcaggcgcc ggaagtcctc gtggccggcg cagccggcca
    12061 ggcggacggc gtccgtgacg tccggataca ggtagaacgt agactccggg gtgtggcagc
    12121 gcacgcccgg cgtcgcgttc agcagccgga gcgccgcgtc gcggcgtccc tggagccggg
    12181 ccatcatctc gcgcggcccg gtctgatcgc cggtgagcgc ctcgagcgcg ccgtactggg
    12241 cgaagtggtt ggggcacgat tcgatgttca cgttcatgcg cgtgatgatc tcgatgatgg
    12301 gcgcgggccc gaccgccgct cccagccgcc agccggtcat cgcgtacttc ttgctgaagg
    12361 tgtagaggat gacggtgcgc tcggccatgc ccgggcgcga gacgatcgac tcgctcgcgc
    12421 cgcggaagcg cacgtcgaag taggcctcgt cgctgagcac gaggaggtcg tgcctcacgg
    12481 cgatctcggc gatgcggtcg agctcctcgg aggtgcactc ggcggccgtc gggttgtgca
    12541 ggtcgttgag gatgaggagg cgggtgcgcg gcgtgatgcg ccgctcgatc gcgtcgatgt
    12601 cgatggtgta gcgctcgccg tcgtcgacgt agccgtaggg cacggcggtc ccgcccacga
    12661 actcgatgaa cgagctgtag atggggaagc cggggttggg atagaggacc tcgtcgccca
    12721 tgttcatcgc cgccatgagc cacttgccga tcaccggctt gccgcccggt tgcaccgaga
    12781 cgttctcggc cgcgtaggtg acgccgcgcg cggagccgac gtcctcagcg agcgcctcgc
    12841 gcagcggcat gatgccggcg ttcggacagt aggtcgtctt gccgtcgcgg acggccctgt
    12901 acatggcctc cacgatgggc tcggccgtgg ggatgttgag gtcgcccagg tggaacgggt
    12961 agatcgtccg accttgggcg gcgagcgcgg ccgcctcggc cgagacggcg aacaccgtct
    13021 cggtcccgag gcgcgccacc cggtccgccg gcacgaagcc gcggccgggc ggggtgaggt
    13081 cttcggcgag cgagtgcatc acatttcctt ccggcgcccc gcgcctcagc tcgcgtccgc
    13141 ggcccaggcg gcgaacttgt cctgctcgcc ttcgagcatg cggtagcagt gctgctcttc
    13201 ggggaaggcg ctgccgcgca cgtcggcgac gtactcggtg agcgccgccg tcgagacggc
    13261 ggcgaggtcg gcgtacttct tcacgaactt gggcgtgaac gcggcgaagg tgccgagcac
    13321 gtcgctgacg atgagcagct gaccgtccac gtcctcgccg gcgccgatgc cgagcaccgg
    13381 gatcggcagc tcgcggcgga tgaagccggc gacctcgggc ggcaccgcct cgacgagcag
    13441 cagctgcacg cccgcctcgt agagggcacg ggcgtcctcg accaccagct tggcggactc
    13501 gagggtgcgc ccctgcgcct tgtggccgcc gagctggccg gagctctgcg gcgtgaggcc
    13561 gatgtggccg cagacggcga cgccggcgtc gaggatcgcc ttgacgcggg agaccgtgcg
    13621 cacgccgccc tcgagcttga tggcgtcgac gttggcctcc ttgaggaagc ggatggcatt
    13681 ggtcaccgcg tcctcgtcgg aggcctggta gctgcccagc ggcatgtcgc cgacgatgaa
    13741 cgtgttcggg gcgccgcggc gcacggcctc gctgtgcacg atgcactggt ccatggtcac
    13801 cggcacggtg cccgggtagc cgtagacgca catgccgagc gagtcgccga cgaggatcat
    13861 gtcgatgccg gccgcctcct cgaacgtggc ggtcgggtag tcgtaggcgg tgagccaggc
    13921 gatcttctcg ccggcgcgct tcatctcgtg caggtcgaag agacctttct tggcgggcat
    13981 gggtggacct cctgtgcagg acgggggaag ccgcggcgag gaccgcgtgc ctattcttct
    14041 acgacgatcg gccagccttc gtcggcgacg gactccgcca ggacgagcat ggtctcgagg
    14101 ttcatgacgc cggggatgcg ggcgatgtcg acgcggatga gctgcccgag gtggtcgagg
    14161 tcgcgcagcg cgagctcgac caccatgtcg aactggccgg tgacgtggta gcaggcgcgg
    14221 acgccgggga cctctttgat gccattctcg aactcgcgga tggactcctc gctgtgctcg
    14281 cgcaggcggg ccacgacgag tgcgcgcaac gtgacgccga gagcggcggg atcgatgacc
    14341 gcgcggtagc cgcggatcgc accgctgcgc tccaggcgac ggactcggcc gagcatggcc
    14401 gacggagaga ggccgagctt gcgcgcgagc gcaaggttgg acatgcgccc gtcctcctgg
    14461 agagccagga gcacttcacg gtcgagagca tccacgccgt ccccctccgg gagccgttgg
    14521 tcgtcggccc cgagtattca gtcttatgtt cgcccagtca agcgcagaag agaacattct
    14581 tccgtaaggt ggccgttgaa ctgttcgaat tgtccgcttc gcctttggaa cggatgcgcc
    14641 catacctcct ccggcgtgag gcccgagggg cgggtgctgc cgggtgagcc gaccgggcag
    14701 cgtcagaggc gctcgcgggc gacgatgtcg gcgagcgcct cgagcacggt gactgcgtcg
    14761 tcgagcacga cggcatcggc ttcgccggcc atggccgagt gctcgtcggt gtcgaccgcg
    14821 acgatgaacc ccgcccgctc gacgccccag aggaaggcag gcgcgcctga ggaccccgcg
    14881 acgatgcaga cggcgggtgc cgcgcgggtg cctgagactc cgatctgccg gtcgggagcc
    14941 gcccaggcgt tcatcacgac cggtcgcgtc accccgaagg ccgcgcccat gcggtccgct
    15001 gcagtggcga tccgctccac gccggcgcgg ccgccggcgc cgcggccggc gacgacgagg
    15061 aactgcgccg cctcgaggtc ggaggtggcc ggcggctcga agacctcgac gtcgtcgcag
    15121 agtctcgggg cggaggagtc gtcctcgcgc agttccgtcg aggcctgcac tacgtgctcc
    15181 gccgggggtt caaagccggc gtcgagccag ccggcgtcca gcgtgacgca ccacgggcgc
    15241 gcgcgcagct cgaaccgccc gatgaggtgg tgcgaggaga cgtgcctgcg acaggagagg
    15301 gcgccggcgg ccgcgaccgc gtccaggacg ccggtgagga cgccgccccc tgagcgggcg
    15361 gccaggcgcg cggccgactc ggcgccgagc gggccgccga agacgaagag ttcggcgccg
    15421 ccgcccgccg cgaaggcggc cagggcggcg gccatcaggt ccggtcggtg cggcggcgcg
    15481 cggaccaggc ggaccagccg ggtcggtgcc agcgcgagca gccgctcgcg ggcgctctct
    15541 tccgcgtaga agaggtgtgt gaccgcgtcc gccggatgct cgggggagaa gcgcagcccg
    15601 tcgcgcagga agccgcccag cgcttgcgcg tgccgctctg cagccgcgcc ggtggcgtcg
    15661 aggacgagcg cgatgcgcgg cgtctcgccg gccatcagat ccgctccagc cgggcatgca
    15721 ggtgctcctc gtacagcgca cgggcctctt ccgcgggcga cgcgcctgcg acgacgacgc
    15781 cggcgcggct ccggtcgatg gtctccagcc cgacgagcgc cgagggctct cgcgagagag
    15841 ctgcagggac gtcgacaccg aggtccgccg gcgtgagcgt ggtgacgacc cggtcccgag
    15901 tcgccagccg gtccttgagc gtcggcacgc gcaacaggga gacgagggcg ttgccgaccg
    15961 ccaggacgca cggcggcgcg atggtcgcgc gcagtggctg gtcgccggcc gagaaggtca
    16021 cgcggaggcg gtccgcggcc ggctcgactg ccgtcacccc gggcaggctc ggccggccca
    16081 gcgcctcggc ggcgaggaag ggtaccaggc cggtgtcacc cgggccgcag cgcgagccga
    16141 ggagcaggac ctcgccgccg aggtgtcgcg tcgcggcggc gaccagcgcc gccgtcgtcg
    16201 ccggggcgaa gtcgaggtcg gcgtccggct cgacccgcgc gccctcgtaa ccgagggcct
    16261 gcagggtcgc gaggaagggg tcggcctcac ggccggcgac ggagaacgcc acaagggagg
    16321 tctcgaggcc gcgctccgtg cgcgcatcgc gcagccgcag cgcgagctcc agcgcggcct
    16381 cgtcgaagac gttgaacacg cgacgtacgt atcggacggc ctcgccgtgc gcactgggcc
    16441 cggtcgcggc ccgggcccag tcggccggcc gcagcgcctc gtaatccggc gtcaccttga
    16501 agcctacggc gatccgcagc gcggtcatgg gatgtggctc ccgccagacc cgctcagagc
    16561 gggatgatcg tccccttgtt catcacgccg ttggggtcga acgagcgctt cagcgtctcc
    16621 agcatggagt aggaggagcc gtactcgtcc ttgacccacg gcgcccgtgc cttgcccatg
    16681 ccgtggtggt ggacgatcga cccgccgcgc ttcagcgtct cctcgcagat gatgccgatg
    16741 atcggcagat agtacttctc ggtctcctcc tcgggcccga cgccgaccac gttgaagaag
    16801 tagttgaagt acatgttggt gccgttgatg tagctgtgcg aggagtgccc gcccatcatc
    16861 gtgatgtccg ggatctcgtc gatgatccgc tgcttcgccg cctggtagat ctcgttgatc
    16921 gagctccagt cggcgctgat ctcggtggtg cggttcacgt tgcgcgtctg gcggatgcgc
    16981 tcgtcctccg cggtgatctt gctcgcgtcc cagcagaggt cgttgaacca cgtctcgatg
    17041 tactcgctgg gcacctgcgt gcactccgga tgctggtcga cgatggcctg gatgccgtcg
    17101 gccgtcgccg tcgcgatccc cgccgcgcct tcggccatga acagcagcac gcagtcgtcc
    17161 ggcgccgcga agtgcgagaa gtggatgccg gcgtcgcccg ggtcgtagac ccgtgccacg
    17221 gacggcttgt acccgttgac catgacgtcg cgcagcacct cgaagccgag ccccatgtcc
    17281 ttgagcgtcc agcccaggaa cacgttgttc tcggggaagt aggggaacag cttgacggtc
    17341 acctcggtga tgaagcagag cgcgccctcg ttgccgatga cgatgtggcg gatgtcgggg
    17401 ccggcggcgc gtcgcggcac gttcttgatg cggcagacct cgccgcccgg gaacacggcc
    17461 tcgaggccga cgaccatgtc ctcgatgccg ccgtacagcg tcgagaactg gccgatgctg
    17521 cgcgtcgcga cgaggccgcc catgtgggcc acaggcttgg actgcggcga gtggccggtc
    17581 gtcaggccgt gctcgcgcgc acggtcctcg agcgcctgca ggggcacgcc gcactggcag
    17641 gtggcctgca tgttgtacgg gtcgaccttg acgatctcgt tcatggccga cccatcgagg
    17701 atcaccgagt cgggcacgac gctctcgagg ccgccctcgg tcgccgtggc gcccgtcctc
    17761 gggatgatgt tgacgccgtt gtcgtcggcc caggtcagga cccgggcgac ctgagccgtg
    17821 tcggccggca gcgctatggc tgccggtacg ggcatggtgt gcacgtcgaa gatgttctgc
    17881 agcttgcgga agttgtccac gctgctctcg acgagcacct tctcgtcggt gacgaccgcg
    17941 tccgggccca cgatgccgcg aaggccctcg acgatggctt ctctgctcag tggcatctcg
    18001 gcgttccttt gcgttccgtg ggttggtcct gggtcagtgg aagatggaga acgactgctc
    18061 gaggatcggg tccgtggctt cgtggatggc gcggtaggcc ttgtcgttca tgcgccggta
    18121 gacatcgtgg ttctccaggt tcggcgcgaa tgactcgcga ctctgcttca tggcgccggc
    18181 ggcggtcggg aagtcgggat acgcaccggt ggccacggcg gcgcagatgg cggatccgag
    18241 gctcgcgccg ctgctgccca cgttgcgcga cgcggggacg ccgaacacgt cggcgaagat
    18301 gttcatgaag agggggctgt tggagccgcc gccggagacg acgatctcgc gcaggtcgat
    18361 gccgagctcg gcgcacatgg cgtcgacgtt ctgtttcatc gtcaaggcga tggcctcgag
    18421 gatcgaacgg tagacgtgcg ccctggtgtg gcgggcgtcg aagccgatga tctcgccctt
    18481 gcggaaaggc ttgtccgtcg gcgcgagcca gtcgagcacc gtcatgagac cgcagctccc
    18541 ggcggggacc agctcggcct cacgctccag atactcctcg cgcgacacct cctgggtcgc
    18601 cgccgccgcc gcgagctccg gccccagcaa gtcgaggaac cagctgagcg tccacatgcc
    18661 gcggcgcacg ccgccgctct cgtagaggta gcggtgcggg atgcaggcga agttcgtcca
    18721 gaactccacc gccgtctggc ggttctcgtg cccgtggacc atggaggcga tgtaggtgcc
    18781 cagcgagacg agcgcggtgc gctcgtcgag cgagccggag ccgagcgcct ccaccgcctt
    18841 gtcgttggcg gtggtcacga ccggcagacc ctccgggatg ccggtcgccg cagccgcctc
    18901 ggcggtcacg tagccggcga cctcgcccgg catctgcagc tcgaccagca gatcgcgagt
    18961 gacgttgaac cgctcgaaca gggcgggatc ccactgccac gtgtcggtgt cgatgggcca
    19021 ctgcaggatg atgttgttgg cggcgctgtc gcggaactgc ccggtgaacc ggtgcgtcat
    19081 gtagcccgag gaggtggtgg cgtaggcgag ctcgggatcg tccgggatcc agggctggta
    19141 ggcgcgcgtg tccatccagc tcatcacggg ctcgagcagc gatccgtcct ccttgaggaa
    19201 ggtcttgcag cagcggatgg tgcagaggcc gacgccgagg atgtcggtgg ggtcgcccgt
    19261 gaacttcgtc atcacctcgc ggctcgccgc cacgatggac tcccagagat cgtcgtcggg
    19321 gtgtacggcg taccccggct gcgggtgctc catggggagg agcgtccgcc tggcctcgca
    19381 gacgatcgtg ccctcgaggt cgtagatgac cactttggtg ctctgggtgc cgttgtcgat
    19441 accgatgaga tacttgccgg ccatcgctca gtccttcccg cgctagcgta cgaggtagcc
    19501 gccgtcgacg accaggacgt ggccgctgac gtagtcggac gccctgccgg cgaggaacac
    19561 ggtggcgccc atgaggtccg ccgggtcgcc ccagcgcgcg gccgggatgt ggtccaggac
    19621 gcgctggttg gtcaccgggt cgctgcgcgt cgccgtggtg atctcggtcg cgtagtaacc
    19681 gggcgcgatc ccgttgacct ggatgtcgta ctcggcgagc tcgtcgcaat aggccttggt
    19741 gaagccggcg aggccgtgct tggtggccgc atacgccgac gaccagcgcc cgccgaggaa
    19801 cgagaagagc gagcagatgt tgatgatctt gccggagcgc tgcgggatca tgaccttggc
    19861 cgcctcgtac gccatctcga aggcggccgt gaggttcacc gcgaccgtcg cgtcccactg
    19921 ctcgcggccg aaatccagga cggcggccag ggggcagatg ccggcgctgt tgacgaggat
    19981 gtcgagcgag ccgagccggt cgacgcaggc ggcgacgatc tctgccggtg cgcccgcggc
    20041 ggtgatgtcc gccttcatga actcgtagcg cccgccgcgc tcctcgacga gtgggccggt
    20101 ctggccgtcg tcgtcgatga tgttgggcac gaagaggtcg gcgccggcag cggcgagcgc
    20161 gagcgtgaat gcctggccga gacctgtgtt gccgccggtg acgatggcgt tcttcccctt
    20221 gagggagaag tagtccatcc cgaactccct gatgtccgtc acgggtctct cctttggcgc
    20281 tgtcggcggg ctctcgggca cgcagtattt ggccggatgt tcgtcaagtc aagccgcccg
    20341 gagaacatcc tgtcgccgcg cgagcgattc gctgcatttc tggcttactc gggctcgagc
    20401 tccgtgggga agccgggcgc gcgaagctcg gtgcccacgg cctcctcgag catcttgacg
    20461 accatgggcg actgcgctcc tccgccgtac atggcgcggg cacgggcgaa gatctgttct
    20521 gtgaggccgc cgagctccag cggcacgccg aactcgcgac ccatctggtg ggcgaagaag
    20581 agatccttgc gggccaggtc catcgtgaag ttgatgttgt agctgccgct caggatcagc
    20641 tgaccctccg tctcgtggac gaagctgttg ccggagctgg ccttgatcac gtcccaggcc
    20701 agcttgaggt cgatgccgcc ctgcttggcg agcatgaggg cctcgccgtc ggcgaccagg
    20761 tggatgaagg cgagcatgtt ggtgatcacc ttgatcaccg aggcctggcc gagctcgccg
    20821 atgtagaaga ctcgcccgcc catggcgtcg aaggcgggcc ggtgcttctc gtacacctct
    20881 tcgtcgccgc cgacgaggac ggtgatcatg ccggcggcgg cgcggtggac accgccggtg
    20941 accggcgcct cgagcgtcgc gacgcccttg gcggcggcga ggctcgagag gcgctgcagc
    21001 tcgtgtgcgt cgttggtgct catgtcgatc caggtcccgc cgggggcgag gttctcgagg
    21061 acgccgcctt cttcggccac cacggaagcc acggcctcgg gggaaggcag gcacgtgatg
    21121 atggtgtcgg aggcctttgc ggtctcggcg atggagccgg cgcaggtggc gcccgccttg
    21181 ccgaggtcga cggagcagct cgcgtccacg tcgtagacgg tgagcgggaa gccggcccgg
    21241 agcaggctgc ccgcgaggtg cctgccgagc gcgccgagcc cgatgtagcc gatcttgagc
    21301 gtcatgagtc ctccgttcgg gagctacgag tgtgtgggcg ccgcgccgtc agctccggcg
    21361 agcgcgccgg agcgcttcgt cgtccgcacg gcagatggcc gcgatctcgt ccagttgcgc
    21421 gccgtcgatg tacggttcct tgctcgcgtg ctcggccagt ctgagctcca cctgggcgca
    21481 cgcgtcgtcg aattccttga cgccgtcctc cgcccacttc tcgtaggaga gccggttcga
    21541 cagcgtcggc atgtagtgct cgccggcgcg gatgcgcttg gcggtctcct tcacgccgag
    21601 gaagccgccg cctgggccca ccgcggcgat ggcctcgccg gcacgcgtgg cctccgacac
    21661 ctccacgggc cggtggaggc ggtgcagcat cgccgccatc tcgttgtcga tgaccgactg
    21721 cggcaacgac atggtgttgt gtgagtcgat caggccccag ccggagacca tgtcgggcac
    21781 ggcgccgcag accgtcgcgg ccttcatcgc cttctcgtag cccgcttgta tgcccgcgtg
    21841 cttggcgtcg gtcgagagcc cagggttgag cgtcggcacg ccgaggtagt ggccgacctc
    21901 ggtggccatg gcccccatca gcccactctc gagtgagccc agcgcgaagg tcgtgcgcag
    21961 catgtcgagc acgcccgagc cgaagcagaa gaccaggcgg gcgcccggtg cggagacctg
    22021 cagagccgtc gcgatgccga ggaactcggc gaggccctgc gtgacgcttc ccgccagtgt
    22081 caccggcgag gtcgctccag cgatcgtcat cgaatacacc tcgatcggca cgccgtgtct
    22141 ggccagggcg atgtgggtgt cgagggcgcc gccgtcgacc tgcagcggcg acgcggccgt
    22201 cacgaccgtc gagatgcgcg gccgggcgcg gaaccgatca aggtcgccgg agagcgcctc
    22261 gcagatgcgc agcacggcgt ccacctcgcc ggggcagtcc acgaaggtca cgtgcttgga
    22321 ggtctcggtg aacatcgtga agtactccgt gagctcacgc tgctcgagcg ggacgtcgga
    22381 cgcgctgacc tgcgtccaca tgatgtcgag ttgcggcagt tcgtccatca gcgccgtgca
    22441 ctcccgcagg tcctgcagcg tgctggcgcg gcgccggccg gtgcggaagt ccagcgtctt
    22501 ggcgacgcag ccgctgggca cgaagtggaa gggctcgccc tcgtccagca gcacgtcgtc
    22561 ggcctcggtg aggccggcca tcaggacgct tcgagggcac tggccggccg cccactccac
    22621 cagttggcgg ggaaggcggg cgatgccggt ggcgtggtct acttgggcgc cgcgctcctc
    22681 gagcagcggc aggaccgcgg agccggcgta acgcatgccc acgcggtcga gcagctcgac
    22741 cgcctcgtcg acgatagcgc tcttttcggc ctcgctcagc cactgcgtcc tggctctcat
    22801 ggatccctcg ccgtccgtgt gggccccttg ggggcgtgtg ctgtgcgggg gtctgcgcct
    22861 ggccgcacgt ttccggggca ctgtagaccg tcctgcaatg attcgcaaga aaagtcgccc
    22921 gacgactgtt gcttctcaac gcataatcgc gataatgcgt tggaacgcaa tgcattgcag
    22981 ggggtcgatt gctgcgaaag gcgcagcgac cgtgcgatgg cggggaggcg gcgatggtca
    23041 ggtggggaca ggcgcccttg gggacgactg aactggacga cctggaccgc cgcatcgtca
    23101 agctcctgcg gccgaatgcg cgccggtcct atgccagcat cgcccgtgtc atcggcgtct
    23161 ctgagcccac ggtgcgcaac cgggtcgacc gcctcatcaa gcgggaagcc atcctcccct
    23221 ggaccggcgt gaacccagcc gcgataggcc tgccggtgga cgtcatggtc ggcatccgcg
    23281 tgaaccgtgg gtacggcaag ggcgtcggtg agagtctcgc ggccatgaag aacgtggcct
    23341 atctcggcta cacgaccggc tccttcgata tcctgatcga ggtgcacctg ccggacaacg
    23401 aagccctgta ccggtttctg aacgaggacc tggaccagat cgagggcatc gcctacaccg
    23461 acacctggca catcctccgc accgacacca cgaacttcga gtgggcgggc gaggacgccg
    23521 gcaacgaacc tctcggggac gaggcgccct gagggtggca cggatccgag ctccacgagt
    23581 cgtcccatgc ggcgatggcg gcggaagacg gcgacgacgg ttgacatcgc tacgcggctc
    23641 ctgtgtagtg gccgctaatt gaccggttga ttagtgcggt cgcctacgtg cgcggccgct
    23701 cgggagggag tgcgcatgac gaacgagccc cgggtgccga cccgcatggg agacggcacc
    23761 ttcacgcatc tgacgcgcag cgagatacgc gcggatctcg tggccgggtc ggagatcgcc
    23821 gccaagcgag ccaaggtggc gccgctcacc gacgacgaga tcgaccacct gctcgagatc
    23881 tacgcctcgc cgctgcgctt catcggcgtg gacgtcggcg aggagatcgt gctgagctgc
    23941 gacggcaccg gcatgaagac ccacgcgacg cgcatccagg acctgcagag ctacgagcag
    24001 tggatgggcg ccgacctgct ggagctctgc gtcggcgact actcgctcaa ggtcgtgcgc
    24061 acgatcctcg cctatgaggc gcagtacatg cacgacgccc agctcgcgac catcgcgccg
    24121 ctgcagtacg gcgtcatgac caacctcggc ctctactcca agccggacgg tccctgcgac
    24181 aactggaacg agctcttgcc gctgggcaag atcgccgagg cccgggcggc tgaggaagaa
    24241 gcgatcgaga tggccgtcgc cgacatggtc aagctggccg actcgcagtg ggaggccggc
    24301 gccgacgcgc tcgactggga caccaccggc gcctcgggcg accccgagtt cctcgccgct
    24361 ctgcgcgcca cgaagatcat ccgcgagaag tacccggaca tgggcatcga gctcggcatg
    24421 gccggcgagt tcgtgctcgg tatgcacggc gagctcgagt ggggcggcaa gcgtctcgcc
    24481 ggcctgtggc ccaaggacca gctgctgctg gcgcaggagg ccggcgccac catgttcggc
    24541 cctgtcgtca acatcaacac cggcaagtcg ctggcctgga acatggcgcg cgccatcgcc
    24601 ctgatcaagc cgtgcatgga cgtggcgacg atccccgtgc accccaacgc cggcatgggc
    24661 gtcggcggcg tgccgatgtt cgtgcacctg ccctccgacg cggtctgccg cgcggcgaag
    24721 tccttcgtcg aactgctgca cgtcgacggg ttgtaggtgg gctcgggcga tccccacggg
    24781 atgcacaacg cccacgcgca agccgcgagc atcggcggta ttcgcgcagc cggagacctg
    24841 gttgcccgta tgcagatgac tcggggcatg cgcctcaagg aagccaagca gtacgtcgcc
    24901 gacaagctcg gcgtgacgcc gttcgacctc tctgatccgg tcgtgatgac cgacgtgcgc
    24961 aacgagcacg ggttcggccg cgtgcacagc tacgcgatca gtcagccctg gcaggccaac
    25021 cagatcgagg cgaagttcaa catcgcccgt gtgctggacg tgccgatcaa cagcgtcgag
    25081 aagttcaagg aacgcacggc gggaggctga ccatgatcag gaacccgcgc gcaggagcgc
    25141 gcaagagcgg cggcttcagg ctcgaggtgt tcaccgagga cgagctgcgg gagatccacg
    25201 gtgcgacgct cgaggtgctg tcgcgcacgg gcgtcttcgt cgaggacgac gaggcccggg
    25261 agaccttcgc cgcggccggc gccgaggtcg acggcgagac gcacgtggtg cgcctgccgg
    25321 cgcacctcgt cgaggacgcg ctgcgctcgg cgccctccac cgtcgtcctg tacggccgcg
    25381 acccggccag ggacgtcgtg ctcgaggacg gtcgggtcgg cttcaccaac ttcggcgagg
    25441 gcatccaggt cgtcgacccg tacaccggcg agctgcacga gagcctgaag gcagacgtcg
    25501 ccgactgcac gaagatcatc gacgcgctgc ccgagatcga cgtggtcgag cggccgctcg
    25561 gcgcccacga cgcgccgccg gacacggcgg cgctgcacaa cgccgaggcc atcttcagca
    25621 acaccaccaa gcacgcgacc atcgggccgc tctcgggctt ctgcgcgcgc aagatgatcg
    25681 acatggcggc ggcgatcgtc ggggggcggg agcagctgcg ccgccgcccc atcctgtctt
    25741 tcctcacctg cccggtgagc ccgctcaagc tggtcaagga cgcctgcgag atcatcatcg
    25801 agggcgcccg cgccggcatc ccggtcaacg tgctctcgat ggcgatggcc ggcggctcca
    25861 gccccgtgtc gctcgccggc acgctcgtca cccacaacgc cgaggtgctg tccggcatca
    25921 cgctggcgca gctcacgcag cggggcgcgc cggtcatcta cggctcctcg accacggcgc
    25981 tcgacctgcg cttcgccagc gcctcggtgg gcagccccga gtgcggcatg atcggcgccg
    26041 gggtcgcctg cctcgcccgc ttctacctgc tgcccagctg ggtcgccggt gcgtagggcg
    26101 actccaaagt ggcggacgcc caatccggtc acgagaagac gatcaccagc ctgctgccgg
    26161 cgttggcagg ggccaacctg atctacgggc tggggatgct ggagtcgggc gtcaccatcg
    26221 attacgcgca gctggtcatg gacgcggagt tcgcgcgcat ggtcaagtac accgtgccgg
    26281 gcatcccggt caccgacgcc acactgtccg tggacgtcat cggcgaggtc gggcccttca
    26341 gcgacttcct ctcgcacgag gacaccctga agggcatgcg cgggcagtcg tcgtccaggc
    26401 tcatcgaccg gcgggtccgc gaggagtgga ccgccgccgg ggcgacgagc atgtacgacc
    26461 gcgcgctcgc cgaggcacgg cgcatcctcg agacgcacgt gccggagccg ctgccggatg
    26521 gcgttgcggc agaacttaag tcgataatcg ccgctgctga gagcgaatta ggagtgcgct
    26581 gactcgcttg acggtgtagt gttcggtctg gcgcgcgacc acctgcggcc gggcgccaga
    26641 ccgaacaacg cctttccagg agcgacgatg tcatacagca gcaaggagat cggcgccggc
    26701 cgcgtcagct tccttaccga agagcagaag cagcgcatct acgccacggc tctcgagatc
    26761 ctcggcgaga tgggcatggt cgtgctccac gacgagggcg agcaggtgat gctcgagggc
    26821 ggctgcacga aggatgcgga cggcaacgtc cgcgtccccg ccgccctcgt cgagcaggcg
    26881 cgcgagtcgg tgccggcggg cttcctggtc tacgaccgcg ccggcgagcc ggcgatggac
    26941 ctcggcggcc tccgctccta cttcggcaac ggctccgacg tcatgcatct acacgacctg
    27001 gagaccggcg agcgccgcct cggaaggctc gcggacgtca ccgaggcggc ccgcctctgc
    27061 gacgcgctgc ccgacatcga cttcgtcatg tcgggcgcct accccgacgg catggacgcg
    27121 agtgaggcgt acccgcggca gttccgcgcc atgatcgccg ccaccgccaa gccgctcgtc
    27181 atgaccgccg gcggcgtcga gaccgtcgag ccgatgtgga agatggcctg cgccgtgcgc
    27241 ggcggcgccg aggcgctgcg ccggaagccg tacttcgtca tgtacaacca gccggtcagt
    27301 ccgctcaagc accctttcga aacggtcgac aagctgctct tctgcgccga ccgcggcatc
    27361 ccgtccacct actgcccgtc gccggtcgcc ggcgggaccg ccccgatcac cactgccggc
    27421 cagatcaccc tgggcatcgc cgaggcgctg ttcgggctcg tgatgcacca gctgcgcgcc
    27481 cccggcgcgc ccttcgtcat cggccagggt cccaacacgc tcgacatggc gacgtcgcag
    27541 tcgtcgtaca actcgcctga gttcatccgc gcctacgcgt gtgaggtcga gatggccaag
    27601 tggctggaca tccccaactg ggggttcagc ggccacaccg acgcccaggt cgtcgacgcc
    27661 caggcgggca tggaggccga tgagatcacc aagctctcca tgcaactggg cgccaacctc
    27721 aaccacgacg tcggctacat ggacttcggc ctctccggct cgctggcgga ggtcgtgctc
    27781 gtcgccgagt tcatctcgcg caaccggcaa ctgctcaagg agatcgaggt cagcgacgag
    27841 accctggccg tcgacgtgat caaggccgtc ggccccggcg gcgacttcct cggtcagcga
    27901 cacacgcgcc gccacctgcg tggcaaccag tggcgcgggc agctgctcaa ccgcgccagc
    27961 tacgaccgct ggatggagaa cggcgggagc gacgtcaccg agaaggcgcg ccagaaggcg
    28021 cgcgacctgc ttgccacgca cgaggtcgcg cctctgccgg agggcctggc cgagaggctc
    28081 gacgcggtgg tgccggccgg ctgagccgcc gccggccgcc ggcgcccggc cgcgtcagtt
    28141 ggccgggacg gtgcgtccct cggcccaggc gcgcaagaag gcgatcttct cgcgcgcggt
    28201 cgaggcgatc ggcggcgtcc gccggagctc gtcgagcagc agggcggtgg tcggagccac
    28261 cttcgccgag aaggccgtgt acagcgccga gatgacgacc tgctccagct ccgccccgct
    28321 gtacccctcg ctctcggctg cgagcagccg gaggtcgaac cggtccggcg cctgcttgcg
    28381 gcgccgcagg tggatgtcga ggatgaccgc cctcgtctct tcgtcgggca ggtcgacgaa
    28441 gaacgtctcg tcgaaccggc ccttgcgcag cagctcggcc ggcaggcgct gcacgtcgtt
    28501 ggcggtggcg accacgaaca cgtcgccgcg gcgctcctgc agccaggaga ggaacgagcc
    28561 caggacccgc agcgacgtcc cgccgtccgc atcctcgccg ccggcggcga acgccttctc
    28621 gatctcgtcg atccacagca ccagcggcgc cagcctgtcc gcggaggcca tggccctgcg
    28681 gaagttctgc tcggtctccc cgatgtactt gtcgtagagc gcgccggggt ccaggcgcac
    28741 gagcggcagg ccccacgccg cggcgaccgc cttggcgcag aggctcttgc cgcagccggg
    28801 cacgccgacg agcagcacgc cgcgcgggaa ctcgaggccg aagtccgcgg cgcgctgccg
    28861 gtcctccatg aacaggcgcc gcttctcgag ccactccttg aggtggacga ggtcggcgat
    28921 gtcgtccagc gagtggaccg cgggctggaa ctcgaggagg ccgtcccgct caacgagctc
    28981 ccgcttggcc tcgacgatgc gtttgacgtc gcccgcgtcg aggcggccgt cctccacgag
    29041 cacacgggtg agaagcttct cggcctccgc gagggtgagg ccctgcaggt tggccagcag
    29101 gcggtcctgg tcggccggcg ccagcgagac ctggaccccc atccgcgtgg agacgtcctt
    29161 gacgacacgc tgctgcaggc tctcgtactc gtcgcgcgtg ggcatgggga ggtgcagcgg
    29221 gccgacttcc acgcgcaggg agcccgggag cctgacgtta gcgccggtca gcacgatcgc
    29281 gcccgagcga ccggcgagct gccgcgccgc ctcgcggagg aggtccgcca cgtcgggcct
    29341 ctccagctcc gggcccaggc cgtggaacac gtagagggcc gggagctgcg agctcacgac
    29401 gtgcccgagc gcggtgcgcg gatcctcggt gccgtaaacg ccggtgggcg cccccgcgcg
    29461 gcgcagcccg gtggcgcgcc tccacacgaa gagaggaagc tgcagctgcc acgcggcgcg
    29521 ttcggcgagc gcctcagcgc ggtcctcctc cgtcgtctcg accagcaggg cgccgtagcg
    29581 ggagcggatc aggagctgga agtcgtgaag ggggtcttgg ctcatcgccg gccctcggcc
    29641 agcgcgagca acgcgatggc gtccgcgctg caggcggtcg cgcagaggcc gcagccgcgg
    29701 cagagggcgg cgtcgaaccc cggccggccg tccgaccggc agacgatggc cgcgaaggga
    29761 cagcgcaggc cgcagcggcc gcaggccttg cagtgattca tgtcgatgcg cgcgatgtgc
    29821 cggcgcacgg gccacgccgc gctcaccccc aggcgatccg ccgcgaggtg cgggaagcag
    29881 cagtcggtgc agcagttgca gatggacgag gtgccggccg ggtcgtcgtg cacgtcggcc
    29941 gtgtgcatca ggcctgcctt gtcggcggtg cgcaggacgt cgcgagcgcg ctcgcgggag
    30001 atctcccagc ccaggccgcg gtcgttctcg aagcgaaggc agacgttcat cggcttgcgg
    30061 caccgggaga cgatcgagcg gcagtcgcag ggccagaggt agatgtggtc ctgggcctcg
    30121 atgatggtct cggcttcctc gaggagaacg taggcgtagc gcgcctcgta ggagtcgccg
    30181 ggacgcccgt ccctggtcgc ctcgacgtcg gggcggatcc ttgtggcgta ggagtcgacc
    30241 tcccagtcgg cgagccggcg gtggacggcg agcgggacgt ccttccagcc ctcgaacatg
    30301 gcccagatct cgaggcgcgc gtggaagtcg gccggcctct ggaccccggc ctcgtcgcgg
    30361 tcgagcacgg cgcggcgtac ggcgcggtcc agctgcgtca cgccgaaacg cgcgagaccg
    30421 tcgtcggctc tcctcgcatc gccgtcgccg gccgcgagga ccacgtcgac gtcgccctgg
    30481 tcgtagaagc ggtcgagcca ggggagcacg aagtccggcg cgccgaccct ctctgccagg
    30541 gtgcgcagcc cggggatcac ctccgcgggt accgcgctac tatccgccac tgcgtctgaa
    30601 tcgcccacgc acgctcctcg ccggcttgtc aggacgccac cgatggtacg tcatcacagg
    30661 ccactcccgc accagggcgg cggctgcgct acgatgggac tcgtggaggg acgattgggg
    30721 cagagtggcg cccgccgagg ccgggttcat cgggcgggcg ctcagctggc cttcctcacc
    30781 cgtcgtgtgt cggatcctga ggagcggctc cgcgcgcatc cgccgtgggg agccggacga
    30841 tgtagatggt gacggcggct gcgaccgcga gcatggcgac gtccgccgcg agcagcggca
    30901 cgaacagaaa gacgctcagc agcacgctcg cccagagcgt agcgagcgcg acgatcttcg
    30961 cccggcgagc gatgccgcgt ccggcctcgt agtcggcgat gtggcgcccg aagagcgggt
    31021 gcgtgaccag ccagcggtgg cagcgctccg aactgcgcac gaagcacgcc gcggcgagca
    31081 gcacgaacgg caccgtgggc aggatcggga ccgcgaggcc gacgacgccc aggccgagcg
    31141 acgtccagcc gcagacgagc aggatcgagg agcgtactcg tggcatgtgg ccagtctagg
    31201 cggcccgcgg ccgccccccc ttgatgctcg tcaacggcgc ggccggcgtc tcctgcgcat
    31261 gcgctccccg cttcccgtca gatgag
//
LOCUS       DASUMX010000173         3599 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1497489, whole genome shotgun sequence.
ACCESSION   DASUMX010000173 DASUMX010000000
VERSION     DASUMX010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3599
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1497489"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            404..1069
                     /locus_tag="VFZ86_08690"
     CDS             404..1069
                     /locus_tag="VFZ86_08690"
                     /inference="COORDINATES: protein motif:HMM:NF024937.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HEX5642399.1"
                     /translation="MTAWILAIGRMLGLGSIAGIRPSLTLAVIGVVGYFDWGVETNAT
                     FSWLNSWLAIGIFVVLAILESAFDKISKVDRLQDRLIMPYRLVMGGVAGAATIPFGWK
                     GVVVGAAVGAGASWFAQYTKHLSRPKSVATPAVVTLISTGEELGAFFGSVLVLAVPYF
                     GYACVAVTGFIYWRVRTLRRSKYRKMRRAAARGKPPAGPADALRDDRAPPAEPGGGEG
                     ADE"
     gene            1062..1694
                     /locus_tag="VFZ86_08695"
                     /pseudo
     CDS             1062..1694
                     /locus_tag="VFZ86_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939472.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
     gene            1827..2096
                     /locus_tag="VFZ86_08700"
     CDS             1827..2096
                     /locus_tag="VFZ86_08700"
                     /inference="COORDINATES: protein motif:HMM:NF012911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEX5642400.1"
                     /translation="MKSVDVRVSGVVQGVYYRAGARREGALRGLRGWVRNESDGSVSL
                     RLQGAPEAVDAMLDWCRVGPPAARVSRLTVADAPADESLHDFEVR"
     gene            2097..2807
                     /locus_tag="VFZ86_08705"
     CDS             2097..2807
                     /locus_tag="VFZ86_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX5642401.1"
                     /translation="MHDGQGTPDHRDTQIRTPVTLDDVRADHEVQVFVRKADENLGVL
                     GYTEHGPRHCALVAEIAGNVLLRLGYPERTAELAAIAGYLHDIGNGINRLDHGIGAAL
                     LSRHILERLGMGPDEYAEVMCAIGNHEEEYGQAVSPLAAAVILGDKSDVHRSRVRVID
                     PETDDIHDRVNMATTRASLVVDPEEHAITLELDIDTGIIQVMQYFEIFLDRMVMGRRA
                     AEFLGCRFGFVINGTRLL"
     gene            2886..3212
                     /locus_tag="VFZ86_08710"
     CDS             2886..3212
                     /locus_tag="VFZ86_08710"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEX5642402.1"
                     /translation="MGKTKKRRGGFLVGAIAGAVAGLLLAPKSGRETREQLFGQGGIG
                     TQVDRIKGAIGAGKDSAAGQNEELRRKIEETRARLRSQMDAGGNGVGGEDAYGGPAGT
                     APGDVS"
     gene            3305..>3599
                     /locus_tag="VFZ86_08715"
     CDS             3305..>3599
                     /locus_tag="VFZ86_08715"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX5642403.1"
                     /translation="MRRVDRRLPAPDADAALDGLTVVQLSDFHAGFTPSFNLRATRKA
                     VDLALAAEPDLVVITGDFAGGAHGLSELRRQLLRLRAPLGVYSVFGNHDHGDSK"
BASE COUNT          501 a         1261 c         1346 g          491 t
ORIGIN      
        1 tgccatgtac gtagcgctgt ctctgtcgac gggtctgcct ctggtgacgg ccgacaaccg
       61 gcttgtgcgg atgctctccg gcacgggctg cgaggtcatt cctctggatg agatcaccgt
      121 gacgtgattc cgcctccgga atgatcaagc gccgcgagag gtctcctgcc gcagccagtc
      181 gcggaccatc tcgccgagca gggcgaaggt cgcgtcgagg tcgacggagt gccgttcgag
      241 gaacgccgcc ccgcacgctg acgccgtgac cgttgcgtgg gccgagtcgg gctggcggcc
      301 tccgcgtcgg atggtcgcgc ctgtggtcgc tccccactgg cacgaggtcc acagacgcgg
      361 cccccgtgac tgacgggtac aatcgagcga tggaggacgc gtcttgaccg cctggatcct
      421 ggcgatcggg cggatgctgg ggctcgggtc catcgccggc atccggccgt cgctcaccct
      481 cgccgtcatc ggcgtcgtcg gctacttcga ctggggcgtc gagaccaacg ccaccttctc
      541 ctggctgaac agctggctgg ccatcgggat cttcgtcgtc ctcgccatcc tcgagtcggc
      601 cttcgacaag atctccaagg tggaccggct gcaggaccgg ctgatcatgc cgtaccgcct
      661 ggtcatgggc ggcgtggccg gggcggcgac catcccgttc gggtggaagg gcgtggtggt
      721 cggggcggcg gtcggcgccg gcgcctcctg gttcgcccag tacaccaagc acctcagcag
      781 gcccaagagc gtggcgactc cggcggtggt gacgctcatc agcaccggcg aggagctcgg
      841 ggccttcttc ggcagcgtcc tggtgctcgc cgtgccctac ttcggctacg cgtgcgtggc
      901 ggtgaccggc ttcatctact ggcgcgtgcg cacgctgcgg cgatccaagt accgcaagat
      961 gcggcgggct gctgcacgcg ggaagccgcc ggccgggccg gccgacgcgt tgcgggacga
     1021 ccgggctcca ccggcggagc cgggcggcgg agaaggcgca gatgagtgac gcgtccgacc
     1081 tcggcgtgcg ccggctgcgg atggtcgagg agcaactcgt ccgccgcggg ctgcgcgacg
     1141 aggacgtgct ctccgccatg cgctcggtcc cgcggcacct cttcgtgccc gaggccgcgg
     1201 ccgcggacgc ctacgccgac tcgccgctgc cgatcggcct cggccagacg atctcgcagc
     1261 cgtacatggt cgcgctcatg accgcgctgc tcgaggtgga cgtccgctca cgcgtgctcg
     1321 agatcggcgc cgggagcggg taccagagtg cggtgctcgg agaggtggcg ggcgaggtct
     1381 tcgcgatcga gcggctggct ccgctggcgg agcgtgctcg cgagacgctc gaccgtctcg
     1441 ggtacgccaa catccgcctg gcgatcggtg acggcgcgag ggggtggcac gagcaggccc
     1501 cgttccaggg gatcctcgtg gccgcggccg ccgacgaggt gccgccggaa ctgctcggcc
     1561 agctcgccga cggcggccgc ctcgtgatcc ccgtgggccg cggtcgcggc gagcaggtgc
     1621 tgaccatcat cgagcgcgac ggcgacgcgt tcatcgagcg gtacgacacg cgctgccgct
     1681 tcgtgccgct catccgcgac gtcagcccgg acgatcgccg cgccgccgct ctgcggggga
     1741 gcggcgcgga cgtggacgcg ggctgggcct cgcccggcgg cgaggacgcc cgcggcgaca
     1801 gcacctaccc tggggaggca cgtgagatga agtccgtgga cgtcagggtc agcggcgtcg
     1861 tgcagggcgt gtactaccgc gccggcgcgc gccgcgaggg ggcgctgcgc ggcctgcgcg
     1921 gctgggtcag gaacgagagc gacggctccg tgtccctgcg cctgcagggc gcgcccgagg
     1981 ccgtcgacgc catgctcgac tggtgccgcg tcgggccgcc ggcggcgcgc gtctcgcgac
     2041 tcacggtcgc ggacgcccct gccgatgaga gcttgcacga cttcgaggtg aggtagatgc
     2101 acgacggcca ggggacgccc gaccacaggg acacgcagat ccgcacgccg gtgacgctgg
     2161 acgacgtgcg cgccgaccac gaggtccagg tgttcgtgcg caaggcggac gagaacctcg
     2221 gcgtgctcgg ctacaccgag cacgggccgc gtcactgcgc cctcgtcgcc gagatcgccg
     2281 ggaacgtgct cctgcgcctc ggctacccgg agcggacggc cgaacttgcc gccatcgccg
     2341 gctacctcca cgacatcggc aacggcatca accgcctgga ccatggcatc ggggcggcgc
     2401 tcctcagccg ccacatcctg gagcgcctcg gcatgggccc ggacgagtac gccgaggtca
     2461 tgtgcgccat cggcaaccac gaggaggagt acggccaggc ggtcagtccg ctggccgccg
     2521 cggtgatcct cggcgacaag agcgacgtgc accgcagccg ggtccgcgtc atcgacccgg
     2581 agacggacga catccacgac cgcgtcaaca tggccaccac gcgcgcgtcg ctggtcgtgg
     2641 acccggagga gcacgcgatc acgctggagc tggacatcga caccggcatc atccaggtga
     2701 tgcagtactt cgagatcttc ctcgacagga tggtgatggg ccgccgtgcc gccgagttcc
     2761 tgggctgccg gttcggcttc gtcatcaacg ggacgcgcct gctgtaagcg gcgcgcggcc
     2821 ggccgccggc gtggcggcgg cgcggccatc tgagatactg ggacgcaagc gaccggaggt
     2881 ggacggtggg caagacgaag aagaggcgcg ggggattcct ggtcggcgcg atcgcaggcg
     2941 ccgtggccgg gctgctcctg gcgcccaaga gcgggcgcga gacgcgcgag cagctcttcg
     3001 gccagggcgg gatcggcacg caggtggacc gcatcaaggg cgcgatcggc gccggcaagg
     3061 actcggccgc cggccagaac gaggagctgc gccgcaagat cgaggagacg cgcgcgcggc
     3121 tgcgcagcca gatggacgcc ggcggcaacg gcgtcggcgg cgaggacgcg tacggcggtc
     3181 ccgccggcac ggcccccggc gacgtcagct gagacggccg gcgcgcaacg ggctgagggc
     3241 ggcggctgcg ggcgccgggg ccgccgcgct gggcctcgtc tacctgctgc acgaggccca
     3301 gtgggtgcgc cgcgtggacc ggcgcctgcc ggcgccggac gcggacgcgg cgctcgacgg
     3361 cctcaccgtc gtgcagctct cggacttcca cgccgggttc acgcccagct tcaacctgcg
     3421 cgccacgcgc aaggccgtcg acctcgcgct ggcggccgaa cctgacctgg tcgtcatcac
     3481 cggcgacttc gccggcggcg cccacggcct gtcggagctc cgccggcagc tgctccgcct
     3541 gcgcgcgccg ctcggcgtct acagcgtgtt cggcaaccac gaccacggcg acagcaagg
//
LOCUS       DASUMX010000174         7958 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1786055, whole genome shotgun sequence.
ACCESSION   DASUMX010000174 DASUMX010000000
VERSION     DASUMX010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 7958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7958
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1786055"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1303)
                     /locus_tag="VFZ86_08720"
     CDS             complement(<1..1303)
                     /locus_tag="VFZ86_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HEX5642404.1"
                     /translation="MSTPAPNPPERGDFIREIVAADLREGRRDTVVTRFPPEPNGYLH
                     IGHAKSICLNFGIAAEFGGRCHLRFDDTNPMKEEQEYIDAIEADIRWLGFDWGEHLYF
                     ASDYFEQLYEWAVELITAGKAYVDDLSADEIRGHRGTFTEPGRDSPWRDRPASESLDL
                     FTRMRAGEFPDGARVLRARIDMAAPNINLRDPVLYRILHAAHPRTGDAWCVYPTYDFA
                     HGQSDAIEGITHSICTLEFEAHRPLYDWLIENLPVPSRPHQYEFARLNLTHTVLSKRV
                     LLRLVNEGTVRGWDDPRMPTMSALRRRGFPAEGIREFATMVGVAKADNTVEVEMLEHC
                     ARDVLNRTALRRFAVLDPLRVIIENYPEGQTEEMEVPNNPEDPSAGSRRVTFSRELWI
                     ERDDFMEEPVRKFFRLAPGREVRLRSAYFVTCDEVVKDDEGR"
     gene            complement(1364..2308)
                     /gene="dmeF"
                     /locus_tag="VFZ86_08725"
     CDS             complement(1364..2308)
                     /gene="dmeF"
                     /locus_tag="VFZ86_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006423992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family Co(II)/Ni(II) efflux transporter
                     DmeF"
                     /protein_id="HEX5642405.1"
                     /translation="MHDDLTGWQHGHSFGQEVRRPGEGRTLVVIAITATMMVVEIVAG
                     LAFASMALLADGLHMASHTAALGIAAFAYVYARRHATDPRYTFGTGKVNALGGFTGAV
                     LLAAFAVFMAAGSIDRLIHPVTIAFDYAIAVAVAGLVVNGVSAVVLSGGRDARGHAGE
                     HAHDDHAHDDHNLRSAYLHVLADALTSVLAIVALLGAKYLGSTWMDPAMGIVGAVLVA
                     RWSIGLLRASGAVLLDRQGPRGLREKIVASIEADGDSRVADLHLWSIGPGVYAAEVSV
                     VAHAPEAADEYKRRLPRGLGVEHLTVEVHGAPADPRAC"
     gene            complement(2511..3293)
                     /locus_tag="VFZ86_08730"
     CDS             complement(2511..3293)
                     /locus_tag="VFZ86_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006859920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX5642406.1"
                     /translation="MNETIRLLDARCSTRAYDPAPPTEEEKRVVLHAAMRAPTAGNMM
                     LYSIIEIEDQALKDRLAVTCDHQPFIARAPWVLLFVADFQKWMDLFAACGCDELPGVP
                     SGVTPGPGDLLMACNDALIAAQNAVVAAESLGIGSCYIGDIRELGETHAELLHLPRYT
                     FPAALLCLGRPKGRPRRTERCEAHVLHKNAYRRLSPSELRAASRELDAIHGPCGSRPG
                     IGGCVQDVYARKFTADFSAEMNRSVAWWLERWCSGGAPAAAE"
     gene            complement(3393..4820)
                     /locus_tag="VFZ86_08735"
     CDS             complement(3393..4820)
                     /locus_tag="VFZ86_08735"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF015536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HEX5642407.1"
                     /translation="MRKPVVTTVLLLLLLAVVVAAAGLGGAATALPAQAGPAASSSHW
                     CNFILAGKDATADGSVLMGYNNDWSVNNYVYMQVVPGAAARNQYVRLLTWGGVPEGGI
                     NVHQLGVNYGTATTLDAAVLAADPFVKKGYGGEIWDIILQQCSTAQEAITLLGQMAQT
                     GFTSGAAGSFGIADPQEAWVFELLGGHHWVAQRVPDDAVLAHPNIVTVRQIDLGDPDN
                     FRGSADLASFAAGIGRYDPAGGPLDVAWAYHDRDVLLSYYNTNRLWGAFDSVAPSLEL
                     DPSMPYADRPVYVVPDDPVTRQDIQAICRYHYEGTAIDQTQDYTLMSPHAQTNRPICH
                     STTDYSAVWQLRSRKPVDIGGVMWVAPSRPCSSAYVPFYGSITSVPSSWTDKTAFNLF
                     RAVADSLDKRGTVDGELRYAHYIPLVQGVYGAFEAECASAQASLETEAARLSGSARIT
                     YLTDYSAQRAMQAQDLASGLPAQMP"
     gene            complement(4924..5928)
                     /locus_tag="VFZ86_08740"
     CDS             complement(4924..5928)
                     /locus_tag="VFZ86_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642408.1"
                     /translation="MSPVLAARLAALRARGAAALIALALAVLALPACGAGGSSVATPP
                     ASPAPASSTSSPAATSTTPSALASPTAQPSGGAVTTAKLARQFPAPSGPLDLRITGSA
                     RWGGVPVRHLTYRSEGAVVTGTLSAPDGAGPFPAVLYAPGVGCSRDMFAADIAALQRQ
                     GIAALVIDPPDGRDPYVRPITTTPDVAAEAHVRYVTDLRRGLDVLRSLPAIDADRLGY
                     VGYSWGGFVGGYLGGLRAPVRAYVLTYAGADWVGADPTAADGFAADPAAAVGAAPGGS
                     YLFIAGVGDPLFSRASVTRYARAAPGGRRIEWLPGGHGDYWAAPAASGMHRSWLLRRL
                     "
     gene            complement(5925..>7958)
                     /locus_tag="VFZ86_08745"
     CDS             complement(5925..>7958)
                     /locus_tag="VFZ86_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEX5642409.1"
                     /translation="LAVPIMFWSPMGTALLGRELPAPFGVSSEVFQFLLSLPVVFYSS
                     AIFFRGAVRALRARTLDMMVLVAVAIGAGWGYSVAVTFWISGEVFYEAAAFLAAFVLL
                     GHWLEMRARGGANEAIRTLLDLAPPMALVLRGGEPVEVPTAEVVVGDELLIRPGAKVP
                     VDAEVLDGESHVDESMVTGESMPVGKRAGDRLVGATINGNGALRARASAVGADTALAQ
                     IVKLVQEAQSSKAPAQRLADRAAFWLVLVALAGGALTFFVWLGPAGAGVSTALLFAIT
                     VVVITCPDALGLATPTAVMVGTGLGARRGILFKSAVALEQMAGLDTVVFDKTGTLTQG
                     RPAVVSVSALGAFGRDEALALAAAVEGQSEHPLAAAIVRAARDGGIPIPPAERFRAVP
                     GHGAVAHVDGRRVVVGNARLLEQEGVGLKGSRASAEQLAGAGRTVVFVAVDGVPAGLL
                     AIADEIRPSARTAVADLRARGLRPVMLTGDTAATAERIAAELGIDEVLAEVLPDGKAA
                     EVEKLQRQGRRVAMVGDGVNDAPALARADVGIAIGAGTDVAVETADVVLMRSDPADVG
                     RAVAVGRGTVRKMRQNLGWAIGYNAIALPIAAGVFAPLGLTLRPEIAAISMSGSSILV
                     AVNALLLKRLDLPPDTAGEAETAAERAPGAAGSGGSSAAGPRQGDDDAVRPAA"
BASE COUNT         1119 a         2797 c         2996 g         1046 t
ORIGIN      
        1 tccggccctc gtcgtccttg acgacctcgt cgcaggtgac gaagtaggcc gagcgcagcc
       61 gcacctcgcg tcccggcgcc aggcggaaga acttgcgcac cggctcctcc atgaagtcgt
      121 cgcgctcgat ccagagctcg cgggagaagg tcacgcggcg gctgcccgcg gacggatcct
      181 ccgggttgtt ggggacctcc atctcctcgg tctggccctc ggggtagttc tcgatgatca
      241 cgcggagcgg gtcgagcacg gcgaagcggc gcagcgccgt gcggttgagc acgtcgcgcg
      301 cgcagtgctc gagcatctcc acctcgacgg tgttgtctgc cttggcgaca ccgaccatgg
      361 tggcgaactc gcggatgccc tccgcgggaa agccccggcg gcggagggcg gacatcgtag
      421 gcatgcgcgg gtcgtcccag ccacgcaccg tgccctcgtt gacgaggcgc agaagcacgc
      481 gcttggacag cacggtgtgc gtcaggttca ggcgcgcgaa ctcgtactgg tgcggccgcg
      541 acggcaccgg caggttctcg atgagccagt cgtagagcgg ccggtgggcc tcgaactcca
      601 gggtgcagat cgagtgcgtg atgccctcga tcgcgtccga ctgcccgtga gcgaagtcgt
      661 aggtgggata cacgcaccag gcgtcgccgg tgcgcgggtg cgcggcgtgg aggatgcggt
      721 acagcacggg gtcgcgcagg ttgatgttgg gcgcggccat gtcgatcctg gcgcgcagca
      781 cccgggcgcc gtcggggaac tccccggcgc gcatgcgtgt gaagaggtcg aggctctcgc
      841 tggccgggcg gtcccgccag gggctgtccc ggccgggctc ggtgaaggtg cctcggtgcc
      901 cgcggatctc gtcggcggag aggtcgtcca cgtacgcctt gcccgccgtg atgagctcca
      961 ctgcccactc gtacagctgc tcgaagtagt cggaggcgaa gtacaggtgc tcgccccagt
     1021 cgaaccccag ccagcggatg tcggcctcga tggcgtcgat gtactcctgc tcttccttca
     1081 tcgggttggt gtcgtcgaag cgcaggtggc agcgcccgcc gaactcagcg gcgatgccga
     1141 agttgaggca gatcgacttg gcgtggccga tgtgcaggta gccgttgggc tccggcggga
     1201 agcgggtgac gacggtgtcg cgccgcccct cgcggagatc ggcggcgacg atctcgcgga
     1261 tgaagtcgcc gcgctcgggt gggttcggcg caggggtact cacagcggcc gaggatagca
     1321 caggcccggc gccgcacgag cgcgactcgt cagctcctac ccgtcagcag gctctcgggt
     1381 ccgccggcgc gccgtgcacc tccaccgtca ggtgttccac gccgagtccc cgggggagcc
     1441 tgcgcttgta ctcatccgcc gcctcaggcg cgtgcgccac cacgctcacc tcggcagcgt
     1501 agacgcccgg cccgatggac cagaggtgaa ggtcggccac ccggctgtcg ccgtccgcct
     1561 cgatgctcgc gacgatcttc tcccgcagcc cccgcggccc ttgcctgtcc agcaggacgg
     1621 cgccgctcgc gcgcaggagc ccgatggacc agcgcgcgac gagcacggct ccgacgatgc
     1681 ccatcgcggg gtccatccag gtggacccca ggtacttggc gccgagcagg gccacgatgg
     1741 cgaggaccga ggtgagcgcg tcggcgagca cgtgcaggta cgcggagcgg agattgtggt
     1801 cgtcgtgcgc gtggtcgtcg tgcgcgtgct ccccggcgtg gccgcgcgcg tcccggccac
     1861 cgctcaggac gaccgcgctg acgccgttca cgacgaggcc ggccaccgcc accgcgatcg
     1921 cgtaatcgaa ggcgatggtg acggggtgga tgaggcgatc gatgctgccc gccgccatga
     1981 agacggcgaa ggctgccagc agcacagccc cggtgaaccc gccgagagcg ttcaccttgc
     2041 ccgtcccgaa ggtgtaccgg gggtcggtgg cgtggcgccg cgcgtacacg taggcgaacg
     2101 ccgcgatccc gagggcggcg gtgtgggagg ccatgtgcag gccatcggcc aggagcgcca
     2161 tcgaggcgaa ggcgaggccg gcgacgatct ccaccaccat catcgtcgcg gtgatcgcga
     2221 tcacgaccag tgtgcggcct tctccgggcc tgcggacctc ctgcccgaag gagtggccgt
     2281 gctgccagcc ggtcaggtcg tcgtgcatgg cgtcaggata ccgggcacgg cgcgcagcgg
     2341 gtacaacccg actgtggcac cgacctcgaa ggagaagcca tgacgatcag agaccggatg
     2401 acgaggcgcg gggcggcggc ggcgctgatc gccgtcatcg cgctcgtgac cttcgcggtg
     2461 ccggcgtggt gcgtcgcctc cggcacctgg agctggtgag cccgcggcgt ctactccgca
     2521 gccgcgggcg ctccacccga gcaccagcgc tcgagccacc acgccaccga gcggttcatc
     2581 tctgcggaga agtccgcggt gaacttacgc gcgtacacgt cctggacgca gccgccgatc
     2641 cccggccgcg atccgcacgg gccgtggatc gcgtcgagct cacgtgacgc cgcgcgcaac
     2701 tccgacgggc tgagccggcg gtacgcgttc ttgtgcaaca cgtgggcctc gcaccgctcg
     2761 gtccgtcgcg ggcgcccctt ggggcggccg aggcacagca gcgccgccgg gaaggtgtag
     2821 cgcggcaggt gcagcagctc ggcgtgcgtc tcgccgagct ccctgatgtc gccgatgtag
     2881 caggagccga tgccgaggga ttccgccgcg acgaccgcgt tctgcgccgc gatgagcgcg
     2941 tcgttgcagg ccatcagcag gtcgccgggg cccggcgtca cgccgctcgg cacaccgggc
     3001 agctcgtcgc acccgcaggc cgcgaacagg tccatccact tctggaagtc ggcgacgaag
     3061 agcagcaccc acggcgcccg cgcgatgaac ggctggtggt cgcaggtcac cgccagccgg
     3121 tccttcagcg cctggtcctc gatctcgatg atcgagtaca gcatcatgtt gccggccgtc
     3181 ggcgcgcgca tcgccgcgtg cagcaccacg cgcttctcct cctcggtggg cggcgccggg
     3241 tcgtaggcgc gcgtcgagca ccgcgcgtcg agcagccgga tggtctcgtt cactgcgcct
     3301 cctccagacg cggcgtgatc gccgctcgac ggaccggggg cggcgcggcg cagccgcccc
     3361 gcccccggtc cgtcaaaccg tcacacgccg ggtcacggca tctgcgcggg cagacccgag
     3421 gccaggtcct gcgcctgcat cgcgcgctgc gccgagtagt ccgtcaggta ggtgatgcgc
     3481 gcggagccgc tcaggcgggc ggcctcggtc tccaggctcg cctgcgcgct ggcgcactcg
     3541 gcctcgaacg cgccgtagac cccctgcacc agcgggatgt agtgcgcgta gcggagctcg
     3601 ccgtccaccg tgccgcgctt gtccaggctg tcggcgacgg cgcggaagag attgaacgcc
     3661 gtcttgtcgg tccaggacga cgggacgctc gtgatcgagc cgtagaacgg cacgtaggcg
     3721 ctcgagcagg gacggctcgg cgcgacccac atgacgccgc cgatgtcgac cggcttccgc
     3781 gagcgcagct gccacacggc gctgtagtcg gtggtcgagt ggcagatggg ccggttggtc
     3841 tgcgcgtgcg ggctcatcag cgtgtagtcc tgggtctggt cgatggcggt tccctcgtag
     3901 tggtaacggc agatggcctg gatgtcctgg cgcgtgaccg ggtcgtcggg gaccacgtac
     3961 acgggccggt cggcgtaggg catgctcggg tcgagctcga gcgacggggc gacgctgtcg
     4021 aacgcgcccc agagccggtt ggtgttgtag tacgacagca gtacgtcgcg gtcgtggtag
     4081 gcccaggcga cgtccaaggg tccgccggcg ggatcgtagc ggccgatgcc cgcagcgaag
     4141 gacgccaggt cggccgagcc gcggaagttg tcggggtctc cgaggtcgat ctggcgcacg
     4201 gtcacgatgt tggggtgcgc gaggacggcg tcatccggca cgcgctgcgc gacccagtgg
     4261 tggccgccga gcagctcgaa gacccaggcc tcctgcggat cggcgatgcc gaagctgccg
     4321 gccgctcccg acgtgaaccc ggtctgcgcc atctggccca gcagggtgat ggcctcctgc
     4381 gccgtggagc actgctgcag gatgatgtcc cagatctcgc cgccgtagcc cttcttgacg
     4441 aaggggtcgg cggcgaggac cgccgcgtcg agcgtcgttg cggtgccgta gttgacgccc
     4501 agctggtgga cgttgatgcc gccctcgggc acgccgcccc aggtgagcag gcggacgtac
     4561 tggttgcggg cggcggcgcc ggggaccacc tgcatgtaga cgtagttgtt gacggaccag
     4621 tcgttgttgt agcccatgag caccgagccg tcggcggtcg cgtccttgcc ggcgaggatg
     4681 aagttgcacc agtgcgagct tgacgccgcc gggccggcct gcgccggaag cgccgtcgcc
     4741 gcgccgccca ggccggccgc cgctacgacg acggccagca ggaggaggag caggactgtg
     4801 gtgacgacgg gcttgcgcat gactcctcct ctggatggcg tgacaacgag gcctttcgtc
     4861 gcccgcccga tatcgcctgc acgaggggcc ggccggcgga agccggcggc gaagaatgtg
     4921 tagtcagagg cggcgcagca gccaggatcg atgcatcccg gacgcggccg gggcggccca
     4981 gtagtcgccg tggccgccgg gcagccactc gatgcgtctt ccgccgggcg cggcgcgcgc
     5041 gtagcgggtc acgctcgcgc gcgagaagag cgggtcgccg actccggcga tgaagagata
     5101 ggacccgccc ggcgcggcgc cgaccgctgc ggcggggtcg gcggcgaagc cgtccgccgc
     5161 cgtggggtcg gcgccgaccc aatcggcgcc cgcgtacgtc agcacgtagg cgcgcacggg
     5221 cgcgcgcagg ccgcccaggt acccgccgac gaagccgccc cagctgtagc ccacgtagcc
     5281 gagccggtcc gcgtcaatcg cgggcaggga gcgcagcacg tcgagcccgc gacgcaggtc
     5341 ggtgacgtag cggacgtgcg cctcggcggc cacatccggc gtggtcgtga tggggcggac
     5401 gtacgggtcg cggccgtccg gcgggtcgat gaccagcgcc gcgatgccct gccgctgcag
     5461 cgcggcgatg tccgccgcga acatgtcgcg cgagcagccg acgccgggcg cgtagagcac
     5521 ggcggggaac gggccggcgc cgtccggcgc cgagagcgtg ccggtgacga cggcgccctc
     5581 gctgcggtag gtcaggtgcc ggaccgggac accgccccag cgggccgagc cggtgatgcg
     5641 gaggtcgagc gggccggacg gggcggggaa ctggcgggcg agcttggcag tcgtgaccgc
     5701 gccgccggac ggctgtgccg tcggcgatgc gagcgcggac ggggtggtcg aggtggcggc
     5761 cggcgacgaa gtcgacgagg ccggcgcagg ggaggccggc ggcgtggcga cggatgagcc
     5821 gccggcgccg caggcgggca gcgcaagcac ggccaaggca agcgcgatga gcgcggcggc
     5881 tccacgagcg cgaagcgccg cgagacgggc ggcgagcacc gggctcacgc cgccggccgg
     5941 acggcgtcgt cgtcgccctg tcgcggaccg gcggcgctgc tcccgccgga gccggcggcg
     6001 cccggcgccc gctccgcggc ggtttccgcc tccccggccg tgtcgggagg caggtccagg
     6061 cgcttgagca gcagggcgtt caccgccacg aggatactcg agccggacat cgagatggcg
     6121 gcgatctccg gccgcagcgt gaggccgagg ggcgcgaaca cgccggcggc gatcggcagc
     6181 gcgatggcgt tgtagccgat cgcccagccc aggttctggc gcatcttgcg caccgtgccg
     6241 cggccgacgg cgacggcgcg ccccacatcg gccgggtcgg agcgcatcag cacgacgtcg
     6301 gccgtctcca ccgcgacgtc ggtgccggcg ccgatggcga tgccgacgtc ggcgcgcgcg
     6361 agcgccgggg cgtcgttgac gccgtcgccg accatcgcga cccggcggcc ctgccgctgc
     6421 agcttctcca cctcggcggc cttgccgtcg ggcagcacct cggcgagcac ttcgtcgatg
     6481 ccgagctcgg cggcgatgcg ctccgccgtc gccgccgtgt cccccgtgag catgaccggc
     6541 cgcaggccgc gcgcgcgcag gtcggccacg gcggtccgtg ccgacgggcg gatctcgtcc
     6601 gcgatcgcga gcaggcccgc cggcacgccg tcgacggcga cgaacacgac ggtgcgaccg
     6661 gcgccggcga gctgttcggc gctcgccctg gagcccttca acccgacgcc ctcctgctcg
     6721 agcagccgcg cgttgcccac gaccacgcgc cgcccgtcca cgtgggcgac cgccccgtgc
     6781 ccgggcaccg cccggaaccg ctctgcgggc gggatcggga tgccgccgtc gcgggccgcg
     6841 cgcacgatcg ccgcggcgag gggatgctcg ctctggccct ccacggcggc ggcgagggcc
     6901 agcgcttcgt cgcgtccgaa ggcaccgagg gcggagacgc tcacgacggc cggccttccc
     6961 tgcgtgagcg tcccggtctt gtcgaagacc accgtgtcca gcccggccat ctgctccagg
     7021 gcgaccgcac tcttgaagag gatcccgcgc cgcgcgccca gaccggtgcc gaccatcacg
     7081 gccgtcggcg tggccagccc gagcgcgtcg ggacaggtga tgacgaccac ggtgatggcg
     7141 aagagcagcg ccgtggagac gccggcgccg gccgggccga gccagacgaa gaaggtgagg
     7201 gcgccgccgg cgagcgccac gaggacgagc cagaacgcgg cccggtcggc gagccgctgg
     7261 gccggcgcct tgctgctctg cgcctcctgc acgagcttga cgatctgggc gagcgcggtg
     7321 tcggcgccga cggcgctcgc ccgcgcccgc agcgcgccgt tgccgttgat ggtcgccccg
     7381 acgagccggt cgccggcgcg cttgcccacc ggcatgctct cgccggtgac catcgactcg
     7441 tccacgtggc tctccccgtc cagcacctcc gcgtccacgg ggaccttggc gccgggcctg
     7501 atcagcagct cgtcgccgac gacgacctcc gcggtcggca cctccaccgg ctcgccgccg
     7561 cgcaggacga gagccatagg cggcgccagg tccagcagcg tgcggatcgc ctcgttggcg
     7621 ccgccgcggg cgcgcatctc gagccagtgg ccgagcagga cgaacgcggc gaggaaggcg
     7681 gccgcctcgt agaacacctc gccgctgatc cagaaggtga cggcgaccga gtagccccac
     7741 ccggcgccga tggccacggc caccaggacc atcatgtcga gggtccgcgc gcggagggca
     7801 cggacggcgc cgcggaagaa gatcgccgac gagtagaaga ccaccggcag gctgaggagg
     7861 aactggaaca cctcggacga cacgccgaag ggcgccggga gttcgcggcc gagcagggcc
     7921 gtgcccatgg gcgaccagaa catgatgggc acggccag
//
LOCUS       DASUMX010000175         3172 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1065969, whole genome shotgun sequence.
ACCESSION   DASUMX010000175 DASUMX010000000
VERSION     DASUMX010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1065969"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="VFZ86_08750"
     CDS             complement(<1..411)
                     /locus_tag="VFZ86_08750"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEX5642410.1"
                     /translation="MRRFDLHALRFHDASEVWRTLPVEVAPFHYGGLEYRVPDDIVEA
                     KLTAARVGENLTLTLEVSTHVLGPCQRCLADADLTVDARGIEYVRHGESGATGEDEDE
                     EAYVVAHQVDLERWVRDVIAEELPVKILCADDCRG"
     gene            complement(448..915)
                     /locus_tag="VFZ86_08755"
     CDS             complement(448..915)
                     /locus_tag="VFZ86_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEX5642411.1"
                     /translation="MDVLVLIDKLDDIIHNARSVPLTDSVMIDREEIYDILDQMRSTI
                     PEEIKQARWIVKERQEMLSEAKQEAERVLAEAQERAEKLASDTEVVRLAEKNAHQIME
                     DAREREREVRLGAEDYADEVLGNLEMNLDKFIAAVRRGRERLQGRGSDDELQP"
     gene            complement(943..1449)
                     /gene="coaD"
                     /locus_tag="VFZ86_08760"
     CDS             complement(943..1449)
                     /gene="coaD"
                     /locus_tag="VFZ86_08760"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEX5642412.1"
                     /translation="MPSNVTAICPGTFDPVTVGHLDIITRGAAKFGRVVVGLIEVPQR
                     KSPLFTPEEREVFLREALAGHDNIVVDRFNELVVEFARRWDAHVILKGLRAISDFEYE
                     FAMAQLNRKLAPDIETVFMMASPEHSFLSSSGVREIAAFGGSVADLVPPAVARRLADI
                     YPPRVLRS"
     gene            complement(1449..2099)
                     /locus_tag="VFZ86_08765"
     CDS             complement(1449..2099)
                     /locus_tag="VFZ86_08765"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016747431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HEX5642413.1"
                     /translation="MRVVGGAWRGRPLSAPPGRGTRPTSDKVREAVFDVLGALPEARE
                     VAASSPSGHAALDVFAGSGALGIEALSRGAGSCTFVESAPPALRALRANLERVGVPVL
                     RGAGSPAPAGTPSPHAVVLAGDVRRVLAADARRGARYTLVFADPPYDLYEQVRPALVR
                     LLGRVLVPGAVLVVESAAGTAAGLPWTVVREKRYGDTRVTFLVADAASPAEGESAH"
     gene            complement(2152..>3172)
                     /locus_tag="VFZ86_08770"
     CDS             complement(2152..>3172)
                     /locus_tag="VFZ86_08770"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF004490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HEX5642414.1"
                     /translation="VTLVLAPLVAACGAVFGKMSGRGLGHTGGTLDKLESIPGFRVQL
                     PQGDFLRQLERIGVAVVSQSERVVPADRLLYALRDVTGTVECDGLIAASIMAKKVASG
                     TSAVVLDLKVGRGAFAADLDHAREIGRLCRVIGTAYGRPTSCLYTAMDEPLGRTVGNR
                     LEVEEAWQVLRGEGPGDVREVTLALAAELLPLADLGVDAREARARAEAALADGRAAEH
                     FERWCYVQGGRWRPGEFHRLAGREVKAARAGYVTAADALAIGRAAQVAGAGRRTVDDA
                     VDPAAGVLLDHAVGDRVEAGEPLATVFSRDPARRREAGLVLERAFVVSEEEPPARTLI
                     LGREE"
BASE COUNT          427 a         1196 c         1116 g          433 t
ORIGIN      
        1 accgcggcag tcgtcggcgc agaggatctt gaccggcagc tcctcggcga tcacgtcacg
       61 cacccagcgc tcgaggtcga cctggtgggc gacgacgtag gcctcctcgt cctcgtcctc
      121 accggtggcc ccggactccc cgtgccgcac gtactcgatc ccgcgcgcgt ccaccgtcag
      181 gtcggcgtcc gccaggcagc gctggcacgg gccgaggacg tgggtgctca cctccagcgt
      241 gagggtgagg ttctcgccca ctcgcgccgc cgtgagcttc gcctccacga tgtcgtcggg
      301 cacccggtac tcgagtccgc cgtagtggaa cggcgcgacc tcgaccggca gcgtgcgcca
      361 gacctcgctg gcgtcgtgga accgcagggc atgcaggtcg aatctgcgca tggtctcaga
      421 ccgcgctcgg cgggcgccgc cggcggctta cggctgcagc tcgtcgtccg agccgcggcc
      481 ctggagacgc tcccggccgc ggcgcaccgc ggcgatgaac ttgtcgaggt tcatctcgag
      541 attgccgagg acctcgtcgg cgtagtcctc ggcccccagc cgcacctcgc gctcgcgctc
      601 gcgcgcgtcc tccatgatct ggtgcgcgtt cttctcggcg agccgcacga cctcggtgtc
      661 ggaggcgagc ttctcggccc gctcctgggc ctcggcgagc acgcgctcgg cctcctgctt
      721 ggcctcggag agcatctcct gccgctcctt gacgatccag cgcgcctgct tgatctcctc
      781 ggggatcgtg gagcgcatct ggtcgaggat gtcgtagatc tcctcgcggt cgatcatgac
      841 gctgtcggtg agcggcacgg atcgtgcgtt gtggatgatg tcgtcgagct tgtcgatgag
      901 aaccaggacg tccatactca gcaaccctcc ttgggtagcc gcctacgagc gcagcacgcg
      961 cggcggatag atgtccgcca gacggcgggc gacggccggg gggaccaggt cggcgacgga
     1021 acccccgaac gcggcgatct ccctgacacc actcgagctc aggaagctgt gctcgggcga
     1081 ggccatcatg aagactgttt cgatgtcggg cgccagcttc ctattgagct gcgccatggc
     1141 gaactcgtac tcgaagtcgg agatggcgcg caagcccttg aggatgacgt gcgcgtccca
     1201 cctgcgggcg aactccacga cgagttcgtt gaagcggtcg acgacgatgt tgtcgtgccc
     1261 ggcgagcgcc tcgcgcagga agacctctcg ctcctccggc gtgaacagcg gcgacttgcg
     1321 ctgggggacc tcgatgaggc cgacgacgac gcggccgaac ttggctgcgc cgcgcgtgat
     1381 gatgtcgagg tggcccacgg tcacggggtc gaacgtgccc ggacagatgg cggtgacgtt
     1441 gctgggcatc agtgtgccga ttctccttct gccggcgacg cggcgtccgc cacgaggaac
     1501 gtgacgcgcg tgtcgccgta gcgtttctcc cgcacgacgg tccaaggcag cccggccgcc
     1561 gtaccggcgg ccgactccac gaccagcacc gcgccgggga cgagcacccg cccgagcagt
     1621 ctgacgaggg cggggcgcac ctgctcgtag aggtcgtacg gcgggtccgc aaacacgaga
     1681 gtatatcgtg cgccgcgacg ggcgtcagcc gccagcaccc ggcgcacgtc gccggccagc
     1741 acgacggcgt gaggcgaggg cgtgcccgcg ggggccggcg acccggcgcc gcggagcacc
     1801 gggacgccga cccgttccag gttggcgcgc agggcgcgca gggcaggcgg cgccgactcc
     1861 acgaaggtgc agctgccggc gccgcgggag agcgcctcga tcccgagggc gccgctcccg
     1921 gcgaagacgt cgagagcggc gtggccggac gggctcgagg ccgcgacctc gcgcgcctcc
     1981 ggcagcgcac caagcacgtc gaacacggcc tcgcggacct tgtcggaggt cggccgcgtg
     2041 ccgcgcccgg gaggcgcgct cagcggccgg ccgcgccacg cgccgccgac caccctcacg
     2101 gccgggcctc cggctcccgg aggagcccgg accccggcgc gccctcgccg gtcactcttc
     2161 cctgccgagg atcagcgtcc gcgccggagg ctcttcctcg ctcacgacga acgcccgctc
     2221 cagcacgaga ccggcctcgc ggcggcgggc cgggtcccgc gagaacacgg tcgcgagagg
     2281 ctctcccgcc tccacccggt cgccgaccgc gtggtccagg agcacgccgg cggccgggtc
     2341 gacggcatcg tccaccgtgc gccgcccggc gccggcgacc tgcgccgcgc ggccgatcgc
     2401 cagggcgtcg gcggccgtca cgtagccggc gcgcgcggcc ttgacctcgc gcccggcgag
     2461 ccggtggaac tcgccgggtc gccagcggcc gccctgcacg tagcaccagc gctcgaagtg
     2521 ctcggcggcg cgcccgtcgg cgagcgccgc ctccgcccgc gcgcgcgcct cgcgcgcgtc
     2581 cacgccgagg tccgccagcg gcagcagctc ggcggcgagc gccagcgtga cctcgcgcac
     2641 gtcgcccgga ccctctcccc gaagcacctg ccacgcctct tccacctcga gccggttgcc
     2701 cacggtccgt cccagcggtt cgtccatggc cgtgtacagg cacgaggtcg ggcgcccgta
     2761 ggcggtcccg atcacccggc agaggcggcc gatctcccgg gcgtggtcca ggtcggccgc
     2821 gaacgctccc cgccccacct tgaggtcgag gaccaccgcg gacgtgccgc tcgcgacctt
     2881 cttggccatg atgctcgcgg cgatgagccc gtcgcactcc actgtgccgg tgacgtcccg
     2941 cagcgcgtac agcagccggt ccgccgggac gacgcgttcg ctctggctca ccaccgccac
     3001 gccgatgcgc tccagttggc ggaggaagtc cccctgcggc agctgcacgc ggaacccggg
     3061 gatcgactcg agcttgtcca gcgtcccgcc ggtgtgcccc aggccgcggc cggacatctt
     3121 gccgaagacg gcgccgcagg ccgcgacgag cggcgccagc accagcgtga cc
//
LOCUS       DASUMX010000176         6330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_493323, whole genome shotgun sequence.
ACCESSION   DASUMX010000176 DASUMX010000000
VERSION     DASUMX010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 6330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6330
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493323"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="VFZ86_08775"
     CDS             complement(<1..501)
                     /locus_tag="VFZ86_08775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642415.1"
                     /translation="MEWSLRRRVIVIVAAAAALAAAGFAAAPAGAATYPPYPSKLVVK
                     SELRFKANLVRDNLIRAPRLVFFGGSRSQRFDPAFARRRTGLSAVNIANSCARPEAAW
                     GYLNWFYKRWPEARIRWVWGMQSGMLRDLDLDPALLQDARFYRYFPDDLLAQQRRLLP
                     DSVAEMP"
     gene            640..1173
                     /locus_tag="VFZ86_08780"
     CDS             640..1173
                     /locus_tag="VFZ86_08780"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEX5642416.1"
                     /translation="MQSRGAYMALSVITGLLMIVLGGLFLAQPGLSLASVIWLVGIFA
                     IVYGLFLAIAGIAGRHEGGGWGIAVGALAVIMGIVTLAWPAATGLAILYVIAAWAIIS
                     GIADIAGAFMGGVSGGRRIWLVIIGLLGIAVGIYFFVHPVTGVLALLWVVGVYMVALG
                     ILRIIAGFMQPPRPQAA"
     gene            complement(1219..1872)
                     /locus_tag="VFZ86_08785"
     CDS             complement(1219..1872)
                     /locus_tag="VFZ86_08785"
                     /inference="COORDINATES: protein motif:HMM:NF024581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX5642417.1"
                     /translation="MTDARDNPYAHIADDTFAAAPRRDPELVKRILEDPRMHDHKDGF
                     QSCIQCGVCTSGCPAARFTGYSPRETARRALDGDVSLLEDDSLWYCYYCYTCQSRCPR
                     HNSVAVINQVVRGMQVENGRGRPHVEMFAEWCGNFYEKGMGGDPHLMFPAIGEAWGEK
                     WTDFMERLLEVRAELGLGDLFPPEDVVDEVRTIFEATGFKERLAAVRGEAEGGGAEA"
     gene            complement(1865..3040)
                     /locus_tag="VFZ86_08790"
     CDS             complement(1865..3040)
                     /locus_tag="VFZ86_08790"
                     /inference="COORDINATES: protein motif:HMM:NF024924.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX5642418.1"
                     /translation="MAAVADTHDYPQAVLDEFERLRQECGICYQCGTCTSSCPSGREL
                     YRGPRRIVRLILAGEIEAVLRSDDLWRCTECGTCTSVCRMGIDVAGILHSLRQLEREH
                     GGIHCAERTAAAVATTHLNKRRRIDNLRFGAAMLSRGYLPKDKVGAAELGMKLAKQMV
                     PGGRKLPAGEALPAGAVTLPFYAGCSIPQDHELHALVHEAAAGFGVRLEEAADAGCCG
                     HPSRGAVPSIFETEERVCTACPACDASLAESGIEAVPLWDALTGLAGRSSSITLRAAG
                     ARFVPYVGCLADRDAALAALGDAAGQAGAAMVVDYPTLHNGCCGALGGMYRGATKSSA
                     RLLQFAARQRAPVVTTCVLCRDNLRSAARELKLDVRVNFWPEYFRAVPTTPEERTDD"
     gene            complement(3024..5465)
                     /locus_tag="VFZ86_08795"
     CDS             complement(3024..5465)
                     /locus_tag="VFZ86_08795"
                     /inference="COORDINATES: protein motif:HMM:NF014692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HEX5642419.1"
                     /translation="MSAAGQGRPLVLLCECAGTLGNIDFDRLERHAGLHADVLRGTHW
                     CSRVGQAQMLELMDAEAEGEAGEGRPLVFAGCSADFAARRFQKLLARGLQLEIADIRE
                     GCSWVHGDDVDAVTDKAARVIDSSIAYPDAQADTMSYAERHDTVVVIGGGVAGTQAAA
                     ELAQMGHHVELVERRPFLGGRAARIGTVFPTNDCGQCLPTTDAQAGTRKCFHRNVAID
                     HPDLSIHRRSTVEAVTGHPGDFQVSIRTLPNIVTDACINCGTCETVCEVESSEPGKKA
                     IFTEFYDGRVIRTVDLETCTFCGACAGECPVEAIDFAQSPSRTVVHAGAILTATGCEP
                     APEKFFDYLGYGHDGVVTQVELAEMLDDWAAQASLGRMPVEELVMVQCAGSRDQRHLP
                     HCSRLCCMIALKHAIRLKALFPAMKVTICYLEMRTAGVGYENWFLDARRAGVEFLRGT
                     PPEVQFDAAGRPVIEVEDVTAARKRVLRPDLVVLSTGMVPAEGTDKLAQTLAVDHDKD
                     GFIEILDRKNRATETSGEGIFVCGSAAGPKALIEVNTEASAVASEIHNFLTSAGRRGT
                     AASRVDASQCVGCDTCQAMCPFGAITLVDRPEGEARPDEVKGDGKLAVIDAQSCRACG
                     ICAANCPEVAIAHNLSDDALFGRIATMTQGVAKPVVGFYCKECAGAAIGLAGQRRDRY
                     PEGVRLIELPCLGRVSALHIVEAARLGAAGVFLAGCAEGRCQYRSGDTSALEQMTIAG
                     EVLAEGGAPLPMELWHLCAVDRMSVGRRIRMFHARAVGDELSDELVEHEMELIGATAS
                     AGAGCAGGEPCGCGR"
     gene            complement(5462..5917)
                     /locus_tag="VFZ86_08800"
     CDS             complement(5462..5917)
                     /locus_tag="VFZ86_08800"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX5642420.1"
                     /translation="MPLTHRGREILDRVPGGSLRSTVGVGCEIVRHEELCIGCGTCAR
                     NCPSGASERGDFFDVNQLLDAPEGSRRGELGAALRRLMQHEPDGPVEVPERVTVYRTI
                     IYDAERCLGCGTCARGCPAGAIEARPPRADAAPAPTAGVSAPAAEGVSA"
     gene            complement(5917..>6330)
                     /locus_tag="VFZ86_08805"
     CDS             complement(5917..>6330)
                     /locus_tag="VFZ86_08805"
                     /inference="COORDINATES: protein motif:HMM:NF014692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HEX5642421.1"
                     /translation="LPPTVRLVLMPCTGRVGPLHLLSALAEGADGVMVAGCLLGQCHY
                     REGNFNAVDRVAFVQRLLKSVGVKPERCRMFTMSAGEPPKFVAAVREMDRVIGGLPPL
                     PRSGDGMSRAPRAGAGAASGAAPSQDLLSATRGAA"
BASE COUNT          839 a         2234 c         2333 g          924 t
ORIGIN      
        1 gggcatctcg gcgaccgagt ccgggagcag ccggcgctgc tgcgccagga ggtcgtccgg
       61 gaagtagcgg tagaagcgcg cgtcctgcag cagcgccgga tccaggtcga ggtcgcgcag
      121 catgccgctc tgcatccccc agacccagcg gatcctcgcc tcgggccagc gcttgtagaa
      181 ccagttcagg taaccccagg cggcctcggg acgggcgcaa ctgttggcga tgttgacggc
      241 gctgaggccc gtgcgccggc gggcgaacgc cgggtcgaag cgctggctgc gcgagccgcc
      301 gaagaagacg agccgtggag cccggatgag gttgtcgcga acgaggttcg ccttgaagcg
      361 aagctccgac ttgaccacga gcttcgaggg atacggcggg taggtggcgg cgccggccgg
      421 cgcggcggcg aagccggctg ccgcgagggc ggccgccgcg gccacgatga cgatcacgcg
      481 gcgcctcagg ctccactcca tcacgctccc cctccctcgg ccggtaccgg ccgtcctctg
      541 cgtgctcctg cggcctgcct ccagtatacc ggtcccccat cctgcgctcc gggtggtaga
      601 gtccggcggc acagaacacc acctcgaaag ggagtaccga tgcagagcag aggcgcctac
      661 atggcgttgt ccgtcatcac cggcctgctg atgatcgtcc tcggcggcct gttcctcgcg
      721 cagcccgggc tgagcctcgc cagcgtgatc tggctggtcg gcatcttcgc gatcgtctac
      781 ggtctgttcc tcgccatcgc cggcatcgcc ggccgtcacg agggcggagg ctgggggatc
      841 gccgtcggcg cgctcgccgt gatcatgggc atcgtgacgc tcgcctggcc cgcggccacg
      901 ggtctcgcga tcctctacgt catcgccgcc tgggccatca tcagcgggat cgccgacatc
      961 gccggcgcgt tcatgggcgg cgtcagcggc gggcgtcgca tctggctggt gatcatcggt
     1021 ctgctgggca tcgccgtggg catctacttc ttcgtccacc ccgtcaccgg cgtgcttgcg
     1081 ctgctgtggg tggtcggcgt gtacatggtc gctctcggca tcctgcgcat catcgccggg
     1141 ttcatgcagc cgccgcggcc gcaggccgcc tgagccgcac cggaccggcg cccggagacg
     1201 ggcgccggtc cggcatcgtc acgcttcggc gccgccgccc tcggcctcgc cgcgcacggc
     1261 tgccaggcgc tccttgaagc ccgtcgcctc gaagatcgtc cggacctcat cgaccacgtc
     1321 ctccggaggg aacaggtcgc cgagcccgag ctcggcgcgg acctccagca ggcgctccat
     1381 gaagtcggtc cacttctcac cccacgcctc cccgatggcg gggaacatga ggtgcgggtc
     1441 gccgcccatg cccttctcgt agaagttgcc gcaccactcg gcgaacatct cgacgtgcgg
     1501 acgtccgcgg ccgttctcga cctgcatgcc gcgcaccacc tggttgatga ccgccacgct
     1561 gttgtggcgc ggacagcggc tctggcacgt gtagcagtag tagcagtacc agaggctgtc
     1621 gtcctcgagc agggagacgt caccgtccag ggcgcggcgc gccgtctcgc gtggcgagta
     1681 cccggtgaag cgcgccgcgg ggcagcctga ggtgcagacg ccgcactgga tgcagctctg
     1741 gaagccgtcc ttgtggtcgt gcatgcgcgg gtcctcgagg atgcgcttca ccagctcggg
     1801 gtcgcgccgt ggggcggccg cgaacgtgtc gtccgcgatg tgcgcgtacg ggttgtccct
     1861 ggcgtcagtc atcggtgcgc tcctcggggg tggtgggaac ggcccggaag tactcgggcc
     1921 agaagttgac gcggacgtcc agcttgagct cgcgcgcggc cgagcggagg ttgtcgcggc
     1981 agaggacgca cgtcgtcacg acgggcgccc gctgccgcgc ggcgaactgc aggagccgcg
     2041 cgctgctctt ggtggcgccc cggtacatgc cgcccaaggc gccgcagcag ccgttgtgca
     2101 gcgtcgggta gtcgacgacc atcgcggcgc cggcctggcc ggccgcgtcg ccgagcgccg
     2161 ccagggcggc gtcacggtcg gcgagacagc cgacgtaggg gacgaagcgg gcacccgccg
     2221 cccgcagtgt gatgctcgag ctgcgaccgg ccagtccggt gagcgcgtcc cagagcggga
     2281 ccgcctcgat gccgctctcc gccaggctcg cgtcgcacgc gggacaggcc gtgcagacgc
     2341 gctcctcggt ctcgaagatg ctgggtacgg cgccccggct cgggtggccg cagcagccgg
     2401 cgtccgcagc ttcttcgagg cggacgccga acccggccgc ggcctcgtgc accaacgcgt
     2461 gcagctcgtg gtcctgcggg atcgagcagc cggcatagaa cggcaacgtg acggcgccgg
     2521 ccggcagcgc ctcgccggcc ggcagcttcc tgccgcccgg taccatctgc ttcgcgagct
     2581 tcatcccgag ctccgccgcg ccgaccttgt ccttcgggag gtagccccgg gagagcatgg
     2641 ccgcgccgaa gcgcaggttg tcgatgcgcc gccgcttgtt caggtgtgtc gtggcgacag
     2701 cggcggcggt gcgctcggcg cagtggatgc cgccgtgctc gcgctccagt tgccgcaggc
     2761 tgtgcaggat gccggcgacg tcgatgccca tccggcacac cgacgtgcag gtgccgcact
     2821 cggtgcagcg ccagaggtcg tcgctcctga ggaccgcctc gatctcaccg gcgaggatga
     2881 gacggacgat gcggcgcgga ccgcggtaca gctcacggcc gctcgggcag ctcgaggtgc
     2941 aggtgccgca ctggtagcag atgccgcact cctgcctgag gcgctcgaac tcgtccagta
     3001 cggcctgcgg gtagtcgtgc gtgtcagcga ccgcagccac agggctcacc tcccgcgcag
     3061 ccggcgcccg ccgaggcggt ggcgccgatg agctccatct cgtgctcgac gagctcgtcg
     3121 gagagctcgt cgccgacggc cctggcgtgg aacatgcgga tgcgccggcc gacgctcatg
     3181 cggtcgacgg cgcagaggtg ccagagctcc atgggcagcg gcgccccgcc ctcggccagc
     3241 acctccccgg cgatcgtcat ctgctcgagc gcgctcgtgt cgccgctgcg gtactggcag
     3301 cggccctccg cgcagccggc gaggaacacg ccggcggcgc ccagacgggc cgcctcgacg
     3361 atgtgcagcg cgctcacccg tccgaggcac ggcaactcga tgagccgtac tccctcgggg
     3421 tatctgtcgc gccgctggcc ggcgaggccg atggcggcgc cggcgcactc cttgcagtag
     3481 aagccgacca ccggcttggc gacgccctgc gtcatggtcg cgatgcgacc gaacagggcg
     3541 tcgtcgctga ggttgtgcgc gatcgcgacc tcggggcagt tggcggcgca gatgccgcag
     3601 gcgcggcagc tctgggcgtc gatgacggcc agcttgccgt cacccttcac ctcgtccggc
     3661 cgcgcctccc cctccggacg gtcgaccagc gtgatggcgc cgaaggggca catggcctgg
     3721 catgtgtcgc agccgacgca ctgggaggcg tcgacgcgcg aggctgcagt gccgcggcgc
     3781 ccggccgagg tcaggaagtt gtggatctcg ctcgccacgg cggaggcctc ggtgttcacc
     3841 tcgatgaggg ccttgggccc ggcggcggag ccgcagacga agatgccctc gccgctggtc
     3901 tcggtggcgc ggttcttgcg gtcgaggatc tcgatgaagc cgtccttgtc gtggtctacg
     3961 gcgagcgtct gcgcgagctt gtccgtgccc tccgccggca ccatgccggt gctgagcacg
     4021 acgaggtccg gccgcagcac gcgcttgcgc gcggcggtga cgtcctcgac ctcgatcacc
     4081 ggccggccgg ccgcgtcgaa ctggacctcg ggcggcgtgc cgcgcaggaa ctcgacgccg
     4141 gcgcggcggg cgtccaggaa ccagttctcg tagcccaccc cggccgtccg catctcgagg
     4201 tagcagatgg tgaccttcat ggccgggaac agcgccttga gccggatcgc gtgcttgagc
     4261 gcgatcatgc agcagaggcg cgagcagtgc ggcaggtgac gctggtcgcg cgagcccgcg
     4321 cactggacca tgacgagctc ctcgaccggc atccggccga gggacgcctg cgccgcccag
     4381 tcgtcgagca tctcggcgag ctccacctgg gtgacgacgc cgtcgtggcc gtagccgagg
     4441 tagtcgaaga acttctccgg agccggctca cagccggtgg cggtgaggat cgcgccggcg
     4501 tggaccacgg tgcggctcgg cgactgcgcg aagtcgatcg cctccacggg gcactcgccg
     4561 gcgcacgcgc cgcagaacgt gcacgtctcg aggtcgacgg tgcggatcac gcggccgtcg
     4621 tagaactcgg tgaagatcgc cttcttgccg ggctcggacg attcgacctc gcagaccgtc
     4681 tcgcaggtgc cgcagttgat gcacgcgtcg gtgacgatgt tggggagcgt gcggatgctc
     4741 acctggaagt cgccggggtg gccggtgacc gcctcgacgg tggagcggcg gtggatgctg
     4801 aggtccgggt ggtcgatggc gacgttgcgg tggaagcact tgcgcgttcc cgcctgcgcg
     4861 tcggtggtgg gcaggcactg gccgcagtcg ttggtgggga acaccgtgcc gatgcgggcg
     4921 gcccggccgc cgaggaaggg ccgccgctcg acaagctcga cgtggtggcc catctgggcg
     4981 agctcggcgg cggcctgcgt gccggccacg ccgccgccga tgaccacgac ggtgtcgtga
     5041 cgctcggcgt agctcatggt gtccgcctgg gcgtccggat aggcgatcga cgagtcgatc
     5101 acgcgggcgg ccttgtcggt gaccgcgtcc acgtcgtcgc cgtggaccca gctgcagcct
     5161 tcgcggatgt cggcgatctc gagctggagg ccgcgcgcca ggagcttctg gaagcggcgc
     5221 gcggcgaagt cggcggagca gccggcgaag acgagcgggc gtccttcgcc ggcctcgccc
     5281 tcggcttcgg cgtccatcag ctcgagcatc tgcgcctgcc cgacgcggct gcaccagtgg
     5341 gtgccgcgga ggacgtctgc gtgcaggccc gcgtgccgct cgagccggtc aaagtcgatg
     5401 ttgccgaggg tgccggcgca ctcgcagagg agcacgagcg ggcgcccctg gccggcggcg
     5461 ctcacgcgct caccccctcg gcggccgggg cggagacgcc ggccgtgggg gccggcgcag
     5521 cgtccgcccg tggcggccgc gcctcgatcg cgccggcggg gcagccgcgg gcgcacgtgc
     5581 cgcagcccag gcacctctca gcgtcgtaga tgatggtgcg gtacacggtg acgcgttccg
     5641 gcacctcgac ggggccatct ggctcgtgct gcatgagccg gcgcagcgcc gcgccgagct
     5701 cgccgcggcg gctgccctcc ggcgcgtcca gaagctggtt cacgtcgaag aagtcgccgc
     5761 gctcggaggc gccgctgggg cagttcctcg cgcaggtacc gcagccgatg cacaactcct
     5821 cgtgccggac gatctcgcag ccgacgccga cggtgctccg cagcgagccg ccgggtacgc
     5881 ggtccaggat ctcgcggccg cggtgggtga gcggcatcag gcagctcccc tcgtcgcgct
     5941 gagcaggtcc tgggaaggcg cggcgccgga cgccgcgccg gccccggcgc gcggcgcccg
     6001 gctcatgccg tcgccggacc gggggagagg cggcaggccg ccgatcaccc ggtccatctc
     6061 gcgcacggcg gccacgaact tgggcggctc gccggcgctc atcgtgaaca tgcgacagcg
     6121 ctcgggcttg acgccgacgc tcttgagcag gcgctgcacg aacgccacgc ggtcgacggc
     6181 gttgaagttg ccctcgcggt agtgacactg ccccagcagg cagcccgcga ccatgacgcc
     6241 gtcggcgcct tcggccagcg cgctcagcag gtgcaggggg ccgacgcgtc ccgtgcaggg
     6301 catgagcacg aggcgcaccg tcggcggcag
//
LOCUS       DASUMX010000177         6395 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1067739, whole genome shotgun sequence.
ACCESSION   DASUMX010000177 DASUMX010000000
VERSION     DASUMX010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 6395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6395
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1067739"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1248)
                     /locus_tag="VFZ86_08810"
     CDS             complement(<1..1248)
                     /locus_tag="VFZ86_08810"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEX5642422.1"
                     /translation="MRTSGREADHVDLDHLDWVEDAPRRTRSGADRRSAERRRGGAKA
                     PAADRRAGERRTAERRSSAGAATRSSGGERAASKPARETARRATKPPAPIAGPGVAER
                     RTLTLVTFLLVVYGLIMAYSASAAQAYFQYGSSFYFFGRQALWVCLGVGAMYVLSRVD
                     YAWFRRAAVPLAGLAFAGLCLVLVPGIGSVINGARRWIIVGGQGVQPSEFAKLACIVL
                     VATLVVRRPREVLTLGGFLKLVVICIVPAAALIMLQPDLGTTLVLVTAVVAVLVASGA
                     PLRYLFGLGAAGCAAVLALIAVEPYRLERLVTFFDPWKDPQGSGFQATQSLISIASGR
                     VFGVGLGNSVQKFGFLPEQTTDMITGIIGEELGLVGLLVLLALYVALAWACFRIALNC
                     KDLFGKLLATGITAMIVGQACINI"
     gene            complement(1255..2676)
                     /gene="murD"
                     /locus_tag="VFZ86_08815"
     CDS             complement(1255..2676)
                     /gene="murD"
                     /locus_tag="VFZ86_08815"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEX5642423.1"
                     /translation="MELADVRCLAVLGLRRTGRPAALLARRELPAARIVGLDEGTPPD
                     DDTLEVLAAAGVEVLTGPAAVLPGAAGLLVKSPGVPETSAVVQEALRRGVPVWSEVEF
                     AARFLGNPIVGITGTNGKTTTTELTGAVFRDARLPVAVGGNIGHALAGMPGEIPPDAT
                     VVAELSSFQLEHIERFRPAVAVLLNLAEDHLDRHGTYRAYVDAKLRIFENQTPDDAAL
                     LCADDAGLLEELAAGRLGGRARRGWFSALTGRADGPGGEELIAGIGADDVLWVRVHGE
                     LRRLCRRDELALRGDHNLLNSLAAAAAACTAGAPVSAAAETLRTFEGVPHRLQVGDVV
                     AGVTWVNDSKATNVDATLKALTAYAGGVHLILGGYDKGAEFAELAAATEGRVKQALLI
                     GATAPRLAAAFAARVAAAGPQATPYVVCGDLQGAVTAAAASAEPGDVVLLSPACASWD
                     QYRDYEQRGEQFLRLVSELRARS"
     gene            complement(2686..3672)
                     /gene="mraY"
                     /locus_tag="VFZ86_08820"
     CDS             complement(2686..3672)
                     /gene="mraY"
                     /locus_tag="VFZ86_08820"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEX5642424.1"
                     /translation="MLRAMGASVLAMVLVLVIGPAFIRWLRVNEFGQNIREDGPEGHK
                     TKEGTPTMGGALIWFAVLIAYLVFSRFSVASLTVFIAALGNALIGFADDWIKIVRKRS
                     LGLSARYKLLLQLLLALFIGFVALRFVGLTTAVDVPFTSYRLELGTLGFYALIFLTLA
                     GFSNAVNLTDGLDGLAAGASAIVLVAMAGIAFIIGRNTSDPGITDLMVVAGCAGGAAL
                     GFLWYNAFPADVFMGDTGSLGLGGAIAGMAVMTRTELVLVIIGGLFVIEAGSVAAQVI
                     SFKLFHRRVLLMAPLHHHFELKAWSETKIIVRFWIIAAIFAGAGFAAYYATF"
     gene            complement(3665..5032)
                     /gene="murF"
                     /locus_tag="VFZ86_08825"
     CDS             complement(3665..5032)
                     /gene="murF"
                     /locus_tag="VFZ86_08825"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEX5642425.1"
                     /translation="MLPLTLGEVAAACGGRLDAPDPHLEVRRVCTDSRRVRSGDLFVA
                     LRGENFDGDEYAAAALAGGAAAAVVRSETAAALPPAAACIVVDDGVAALQALATSVRR
                     RSGVKVVGITGSAGKTSTKDILAALLRPVARTVATSANLNNEIGVPLTLLEIEPDTEV
                     AVVEMAMRGAGQIRELARVALPDVGVITNIAPVHLELVGTIEDVASAKAELVEELTDG
                     TAVVPADEALLDRHVRRHRGRVVTFGTPDADVHVVDEERRGESTHVLLDAFGHRARMD
                     FSFTGGHYLRDALAAVAAFVELGYRLDQAAEGAAQVAFSDLRGALSALPGGGLLLNDA
                     YNANPVAMKAAVDHLVAIADGRPTVAVLGDMYELGPGAGTYHRAVGEHCAAAGVRLVA
                     VGDLARDYLTGAPGERWFPTLDECLAALPEVATPGSAVLVKASRLMRMERVAEALSGE
                     GAPHA"
     gene            complement(5037..>6395)
                     /locus_tag="VFZ86_08830"
     CDS             complement(5037..>6395)
                     /locus_tag="VFZ86_08830"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001124.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEX5642426.1"
                     /translation="GHAFAAAAVEAGAAALVCERDTGVGAPQVVVPSVRRAMALAAAR
                     WYGDPSRELRVVGVTGTNGKTTTAHLVAGLFAAAGEPSGLLGTVVNRIGGVEHPVKLT
                     TPESLDLQRMFREMAEAGDRACAFEVSSHALAQDRAAGIAFDAVVFSNLTRDHLDYHK
                     DLEDYFGAKRRLFLPDEGRNGHAVAVVNVADEFGARLARECRPAYGDDLWTCAVDEEG
                     ASGAVVVARDLQLRADGSSFSLVCSRLGIDERVSLRLAARFNVENALAAAAAGLALGL
                     PVEAVLRGLAVTEGVPGRFQAVRAGQPFSVVVDYSHTPDSLENALQAARAVTSGRVLV
                     VFGCGGDRDRGKRPLMGGIGARLADRAVVTSDNPRTEEPLAIIDEIAAGVPRELAPRV
                     VIEPDRREAIRIALAEARPDDTVVIAGKGHEQGQLIGDRKIPFDDRAVAEEELARLSG
                     EW"
BASE COUNT          904 a         2299 c         2401 g          791 t
ORIGIN      
        1 gatgttgatg cacgcctggc cgacgatcat cgccgtgatg ccggtcgcca gcagcttgcc
       61 gaacaggtcc ttgcagttga gcgcgatgcg gaagcacgcc cacgccagcg cgacgtacag
      121 ggcgagcagg acgagcaggc cgacgagccc gagctcttcc ccgatgatgc cggtgatcat
      181 gtcggtggtc tgctcgggca ggaacccgaa cttctgcacg gagttgccga ggccgacccc
      241 gaacacccgc ccggaggcga tcgagatcag cgactgcgtc gcctggaacc cgctgccctg
      301 ggggtccttc cacgggtcga agaacgtgac caggcgctcg agccggtacg gttccacggc
      361 gatgagcgcg agcacggcgg cgcagcccgc cgcgcccagc ccgaacaggt agcgcagggg
      421 cgcccccgag gcgaccagga cggcgacgac ggcggtcacc aggacgagcg tggtgccgag
      481 gtccggctgg agcatgatga gcgcggccgc ggggacgatg cagatgacga cgagcttcag
      541 gaaccccccg agcgtcagga cctcccgggg ccgcctgacg acgagcgtcg ccacgagcac
      601 gatgcaggcg agcttggcga actcgctcgg ctgcaccccc tgcccgccca cgatgatcca
      661 gcgccgggcg ccgttgatca cgctgccgat gcccgggacc aggacgaggc acaggccggc
      721 gaaggcgagc ccggcgagcg ggacggcggc cctgcggaac caggcgtagt ccacgcgcga
      781 gagcacgtac atggcgccga cgccgaggca gacccagagc gcctggcggc cgaagaagta
      841 gaagctgctg ccgtactgga agtacgcctg cgcggccgag gcgctgtagg ccatgatgag
      901 gccgtagacg acgagcagga aggtgacgag cgtgagcgtg cgccgttcgg cgacgccggg
      961 gccggcgatg ggcgccggcg gcttcgtcgc gcggcgggcc gtctcgcgtg cgggcttgct
     1021 tgccgcccgc tcacccccgc tgctgcgggt cgcggcgccc gctgaggagc gccgctcggc
     1081 cgtgcgccgc tcgccggcgc gccggtccgc ggcgggagcc ttggcgccgc cgcggcggcg
     1141 ctccgcgctg cggcggtcgg cgcccgagcg cgtacgtcgc ggagcgtcct cgacccagtc
     1201 gaggtggtcg agatcgacgt gatccgcctc gcgtcctgac gtcctcactc ggcgtcagct
     1261 ccttgctctc agctcgctca ccagcctgag gaactgttcg cctcgctgct cgtagtccct
     1321 gtattggtcc cagctcgcgc aggcgggact gagcaggacc acgtcgcccg gctcggccga
     1381 ggcggccgca gcggtgacgg cgccctgcag gtcgccgcag acgacgtagg gcgtcgcctg
     1441 aggtccggcc gcggccaccc gggcggcgaa cgccgccgcc agccgcggcg ccgtcgcgcc
     1501 gatgagcagc gcctgcttga cgcggccttc ggtcgcggcg gcgagctcag cgaactccgc
     1561 gcccttgtcg tagccgccca ggatgaggtg cacgcccccc gcgtaggcgg tgagcgcctt
     1621 gagcgtcgcg tccacgttgg tcgccttgga gtcgttcacc caggtcaccc cggcgacgac
     1681 gtccccgacc tgcaggcggt gcgggacgcc ctcgaacgtg cgcagggtct ccgcggccgc
     1741 cgagaccggc gcgccggcgg tgcaggcggc ggccgcggcg gcgagcgagt tcagcaggtt
     1801 gtggtcgccg cgcagcgcca gctcgtcgcg gcggcagaga cggcgcagct cgccgtgcac
     1861 ccgcacccac agcacgtcgt cggcgccgat gccggcgatg agctcctccc cgcccggccc
     1921 gtcggccctc cccgtcaggg cggagaacca gccgcgccgg gcccggccgc ccagccgccc
     1981 ggcggcgagc tcctcgagga ggccggcgtc gtccgcgcac agcagcgccg cgtcgtcggg
     2041 cgtctggttc tcgaagatgc gcagcttcgc gtccacgtag gcgcggtagg tgccgtgccg
     2101 gtcgaggtgg tcctccgcga ggttgaggag gacggccacg gccggccgga accgctcgat
     2161 gtgctcgagc tggaagctcg acagctcggc caccaccgtc gcgtccggcg ggatctcgcc
     2221 gggcatgccg gccagggcgt ggccgatgtt cccgccgacg gccaccggca ggcgggcgtc
     2281 gcggaagacg gcgccggtga gctcggtcgt cgtcgtcttg ccgttggtgc cggtgatgcc
     2341 cacgatgggg ttgccgagga agcgggccgc gaactcgacc tcgctccaca ccgggacgcc
     2401 gcggcgcagc gcctcctgga ccacggcgct cgtctccggg acgccggggc tcttgaccag
     2461 caggccggcg gcgcccggca gcaccgccgc cgggccggtc aggacctcga ccccggcggc
     2521 cgcgagcacc tcgagcgtgt cgtcgtccgg cggcgtaccc tcgtcgaggc cgacgatccg
     2581 cgccgccggc agctcacgcc gggcgagcag cgccgccggc ctgccggtcc tgcgcaggcc
     2641 gagcacggcc aggcagcgca cgtcggcgag ctccatcgtc ccccctcaga aggtcgcgta
     2701 gtaggccgcg aacccggcgc ccgcgaagat ggcggcgatg atccagaacc gtacgatgat
     2761 cttcgtctcg gaccacgcct tgagctcgaa gtggtggtgc aacggcgcca tgagcagcac
     2821 gcggcggtgg aacagcttga acgagatgac ctgcgcggcc acggacccgg cctcgatgac
     2881 gaagaggccg ccgatgatga ccagcacgag ctcggtgcgc gtcatgaccg ccatgcccgc
     2941 gatcgcgccg ccgaggccga gcgagccggt gtcccccatg aagacgtcgg ccgggaacgc
     3001 gttgtaccag aggaagccga gcgccgcgcc gccggcgcac ccggcgacga ccatgaggtc
     3061 ggtgatgccc ggatcgctcg tgttgcgccc gatgatgaag gcgatgcccg ccatggcgac
     3121 cagcacgatg gcggaggctc ccgccgccag gccgtcgagg ccgtcggtga gattgacggc
     3181 gttggagaag ccggcgagcg tgaggaagat gagcgcgtag aacccgaggg tcccgagttc
     3241 gagccggtac gaggtgaacg gcacgtcgac cgccgtggtg aggccgacga agcgcagcgc
     3301 gacgaagccg atgaacagcg ccagcagcag ctgcaggagc agcttgtagc gcgcggacag
     3361 gccgagcgag cgtttgcgca cgatcttgat ccagtcgtcg gcgaagccga tcagcgcgtt
     3421 gcccagcgcg gcgatgaaca cggtcagcga ggcgaccgag aagcggctga acaccagata
     3481 cgcgatgagg acggcgaacc agatgagcgc gccacccatg gtcggcgtgc cctccttggt
     3541 cttgtgaccc tccgggccgt cctcgcggat gttctgcccg aactcgttga cgcgcagcca
     3601 gcggatgaac gccggcccga tgaccaggac cagcaccatg gccagcacgc tcgcgcccat
     3661 ggccctaagc atgcggcgct ccttctccgg agagcgcctc ggccacgcgc tccatgcgca
     3721 tgaggcgcga ggccttgacg aggacggcgc tgcccggcgt cgcgacctcg ggcagcgctg
     3781 ccagacactc gtccagcgtc gggaaccagc gctcgccggg cgcccccgtg aggtagtcgc
     3841 gggcgaggtc gccgacggcg acgaggcgca cgccggcggc ggcgcagtgc tcgccgacgg
     3901 cgcggtggta cgtgcccgct cccgggccga gctcgtacat gtcgccgagc accgccaccg
     3961 tggggcgccc gtcggcgatc gcgacgaggt ggtccacggc ggccttcatg gcgacgggat
     4021 tggcgttgta ggcgtcgttg aggagcaggc cgccgcccgg cagcgcggag agagcgccgc
     4081 gcaggtcgct gaacgccacc tgcgcggcgc cctccgccgc ctggtcgagg cggtagccga
     4141 gctccacgaa cgcggccacg gcggccagcg cgtcgcgcag gtagtggccg ccggtgaagc
     4201 tgaagtccat gcgcgcgcgg tgcccgaacg cgtcgagcag cacgtgtgtg ctctcaccgc
     4261 ggcgttcctc gtccaccacg tgcacgtcgg cgtcgggcgt cccgaacgtg accaccctgc
     4321 cgcggtgccg gcgcacgtgg cggtccagca gggcctcgtc ggccggcacg accgccgtcc
     4381 cgtcggtgag ttcctcgacc agttccgctt tggcggaggc gacgtcctcg atggtgccga
     4441 cgagctccag atggaccggg gcgatgttgg tgatcacgcc gacgtccggc agcgccacac
     4501 gggcgagctc gcggatctga ccggcgccgc gcatcgccat ctcgacgacc gcgacctcgg
     4561 tgtccggctc gatctcgagc agcgtgagcg gcacgccgat ctcgttgttg aggttcgcgg
     4621 aggtggccac cgtgcgggcg accggccgca gcagcgccgc gaggatgtcc ttggtggaag
     4681 tcttgccggc gctgccggtg atgccgacga ccttgacgcc ggagcggcgg cggacggagg
     4741 tggccagcgc ctgcagcgcg gcgacgccgt cgtcgacgac gatgcaggcc gcggccggcg
     4801 gcagggccgc cgcggtctcg ctacgcacga cggccgccgc ggcgcctccg gcgagggcgg
     4861 cggcggcgta ctcgtcgccg tcgaagttct cacctctgag ggcgacgaac agatcacccg
     4921 agcggacgcg gcggctgtcg gtgcacacgc gccgcacctc gaggtgggga tcgggggcgt
     4981 cgaggcggcc gccgcaggcg gccgcgacct cgccgagcgt cagcggcagc accgcgtcac
     5041 cactccccac tcagacgcgc gagctcttcc tcggccacgg cgcggtcgtc gaacgggatc
     5101 ttgcggtcgc cgatgagctg tccctgctcg tgccccttgc cggcgatgac gacggtgtcg
     5161 tctggccgcg cctcggcgag agcgatgcgg atggcctcgc gccggtccgg ctcgatgacg
     5221 acccggggcg ccagctcccg cggcacgccc gcggcgatct cgtcgatgat cgcgaggggc
     5281 tcctcggtgc gcggattgtc ggaggtgacg accgcgcggt cggcgaggcg ggcgccgatg
     5341 ccgcccatga gcggccgctt gccgcggtcg cggtcgccgc cgcagccgaa gaccacgagg
     5401 acgcgccccg acgtgacggc gcgagccgcc tggagcgcgt tctcgagcga gtcgggcgtg
     5461 tgcgagtagt cgaccacgac gctgaagggc tggccggcgc gcaccgcctg gaagcggccg
     5521 gggacgccct cggtgacggc gagtccgcgc aggacggcct cgacgggcag cccgagggcc
     5581 agcccggccg ccgccgcggc gagggcgttc tcgacgttga agcgcgccgc gagccgcagc
     5641 gacacacgct cgtcgatccc gaggcgcgag cagacgagcg agaagctgga gccgtcggcg
     5701 cgcagctgca gatcgcgggc gacgacgacg gcgccggagg cgccctcctc gtcgaccgcg
     5761 caggtccaga ggtcgtcgcc gtaggccggg cggcactccc gcgccaggcg cgcgccgaac
     5821 tcgtccgcca cgttgacgac tgcgacggcg tggccgttgc ggccctcgtc cggcaggaag
     5881 agccggcgct tggcgccgaa gtagtcctcg aggtccttgt ggtagtcgag gtggtcgcgc
     5941 gtcaggttgc tgaagacgac cgcgtcgaag gcgatgccgg cggcgcggtc ctgggcgagg
     6001 gcgtgcgagc tgacctcgaa cgcgcacgcc ctgtcccccg cctccgccat ctccctgaac
     6061 atgcgctgca ggtcgaggga ctccggcgtg gtgagcttga cggggtgctc gaccccgccg
     6121 atgcggttga cgaccgtgcc gaggagcccg gacggctctc ccgcggcggc gaacaggccg
     6181 gccacgaggt gcgccgtggt cgtcttgccg ttcgtcccgg tgacgccgac cacgcggagc
     6241 tcgcgcgagg ggtcgccgta ccagcgcgcc gccgccagcg ccatcgcgcg gcgcaccgac
     6301 gggacgacga cctgcggcgc gccgacccca gtgtcgcgct cgcacacgag cgccgcggcg
     6361 ccggcctcca cggccgcggc ggcgaacgcg tgacc
//
LOCUS       DASUMX010000178        10638 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_494213, whole genome shotgun sequence.
ACCESSION   DASUMX010000178 DASUMX010000000
VERSION     DASUMX010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 10638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10638
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494213"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..906
                     /locus_tag="VFZ86_08835"
     CDS             118..906
                     /locus_tag="VFZ86_08835"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX5642427.1"
                     /translation="MADSATDGDQRRIGVQVIARAAEICRELATATDGLTTAELVERL
                     GLSRPTVYRILRALMDEDFVRQVAAGRYAIGPAFLVIGGSSYGGLRHDLRPFMRDLSN
                     ELGETIDLAVLDSGEALFVDQYVAVQGLRVVAHMGARLPLYCTASGKALLAELSPGEA
                     ERELPETLEAYTPNTGTDRAALLRELEEVRRTGVAYDNEEYVMGVCAVAAPVRDAVGA
                     VGALTVEVPASRFSGREDTLTRAVLRQRDLAHRALVGAGQTRRD"
     gene            1198..3810
                     /locus_tag="VFZ86_08840"
     CDS             1198..3810
                     /locus_tag="VFZ86_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005031368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX5642428.1"
                     /translation="MGEAVRLTNSSTGGPVFVYVEDGKIVRITPMDLDDAVDAASWKI
                     EARGKTFRPPRKTTYSVYTAGFKSMIYSDRRIKTPMKRVDFDPDGERNIQNRGISGYE
                     PISWDEATDIVVSEINRVKREAGPAAILSSPSSHHMWGNVGYRHSTYFRFMNMMGFTY
                     ADHNPDSWEGWHWGGMHMWGFSWRLGNPEQYDLLEDTLENTEMIVFWSSDPETNTGIY
                     SAHESTPRRFWMKDLGIKMVFIDPFYNHTAGLIGDKWFSPKIGTDHALSFAIAHTWLT
                     EDTYDKAYVATHAHGFDEWADYVLGKTDGVPKTCEWAEAESGVPACEIRALAREWAKK
                     KTMLAAGGLGGWGGACRASHGIEWSRGMIALATMQGMGKPGSNIYSTTQGTPVDYDFY
                     FPGYAEGGISGDCENSAAGFKFAWRMFDGKTTFPSPSNINTSAGQHIPRLKVPECIMD
                     GEFQWAGKGFAGGDIQHQLHQYRYPAPGYPRIKMLWKYGGPWIGTMTATNRYARMYAD
                     PSLEFVVSQSIWFEGEVPFADIILPACTNFERWDISEFANCSGYIPDTYGQCNHRVIS
                     LQAKCIEPVGESKSDYQIYAMIAEKLGIGPMFTEGKDELAWVEQYYNATDMPKHMSFA
                     EFKEKGYFIVPDNPERKKTPALRWFAEDREKDTPDWGPRLNNQVGRKGLQTSTGKVEF
                     IATSLKNFEDQGFVDEYRPSMHTYVPAWEGPACELARKYPLGMLSPHPRFSFHTMGDG
                     KDSFMNDIKDHRVLVDGHYYWIIRMNRLDAAARGIRSGDLVRAFNDRGSVIVCAQVGE
                     RMQPGTVHTYESCAEYDPLGKPGRSADRGGCMNILTPDRYMSKYASGMAPNTAQIEVE
                     KWDGGIYETEESVQ"
     gene            3807..4688
                     /locus_tag="VFZ86_08845"
     CDS             3807..4688
                     /locus_tag="VFZ86_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005031370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX5642429.1"
                     /translation="MKQWYKVIDVARCHDCNNCFMADKDEFVGNDWPGYTAAQPRHGH
                     RWVDILRRERGQYARNDVAYLPVPCQHCENAPCIDASDGAITKREDGIVMIDMVKAKG
                     NKALAQSCPYHAIWWNEEADIAQKCIFCAHLIDDPTWTPHTTRCTHSCPTDAMATYFV
                     EPEEMARMIAAEKLEAYLPDLGTKPHTLYKNLFRFTLNFITAGVLVDGDCYEGATVTL
                     RSHEGGIACDAGGAGMADGGDTATLARRRTNFFGEFKFDGLEYGEYELEVDAGGKRYA
                     ARVLVDNESQNLGFIEL"
     gene            4801..5355
                     /locus_tag="VFZ86_08850"
     CDS             4801..5355
                     /locus_tag="VFZ86_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005031371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HEX5642430.1"
                     /translation="MTKIVVFNGSPRMDGNTTTILDLVAKGARDRGAEVKYYTLFKMK
                     FMACQSCFACRVKQDCVINDELREALHEVTAADAVVIGSPVYMMQMTGPVKNLYDRLF
                     PLMDGEGRPRFGAKKMISVYSQSVDDVHAYDSYFEYTAAMYPSFGFEWVDNIVCTNAN
                     DPESADHDAELQIRAYEAGRALAR"
     gene            5585..8617
                     /locus_tag="VFZ86_08855"
     CDS             5585..8617
                     /locus_tag="VFZ86_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008621094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX5642431.1"
                     /translation="MAGTDGRRVARAVGQAKHAAGRAKKTAAEAQFTATLAGLEVALR
                     ATAGRYPAFAARLRERDLVAQIRLAGRARGRAYTIKGGRVRSRAGLHPAPDLTMTFDS
                     AEVAARIMRPGRDYLEFIDALKNFQMVVEGPDELAVWFSETLQMMLTAGLEFGVDAGA
                     GVRRFTSNTNGGPVFVYVRDGRILRITPIEFDEDDAEAWTIEARGRSFTPPRKTTVSS
                     HTLAWKSLIYSPDRLLHPMKRVDFDPDGERNPQNRGTSGYERISWDEALDLVASEIKR
                     VKRDHGPGAIMNGSGSHHTWGVLGYWLSARIRFFNMLGWSPVMHNPDSWEGWYWGAAH
                     HWGQSARNGGGETYGTVEDLLKHAEMVVFWSADPEATSGVYGAHDGTIRRQWLKDLDI
                     PCVHIDPFHNHTAQLLGGKWIAPRPGTDSALVLAIAHVWITEGLYDAGYVAERTVGFE
                     KWRAYILGEEDGAGKTPEWQEAETGVPAREVRALARAWGKKKTYLAAGGLIGFGGACR
                     TATGSDWARGVVCLMAMQGLGKPGVNMGCLQQGTPLDTHFFFPGYAEGGYSGDLYGTA
                     LSINMYQRMPQLATVNTVSQVVPRLKIPEAVMDGHCEAYPTDPKSIEGQFQRYEYPAP
                     GHSRIKMYYKYGGSHFGTMTDTNRFARMYRSDKLEFVVNQGIWMEGETKFADVILPAC
                     TNFERWDISEFANCGGYIQHAYTQCNHRVAVMQHKCIEPLGESRSDHQIFLDLATRLG
                     LGSMYSEGVTELDWCKRLFDATDLPRSVSWKDFLKKGYYVIPPPPEERRDPVSYRWFA
                     EGRAKDTPELTPLPADYTEEFGMGLQTQSGKLEFEASSLKRFDPDDPERAPIMRYVPA
                     WEGPHAAELYGKYPLQLITPHPRHSFHTQSDGKDAVVNDVRDHRVLVDGRYYWIVRVN
                     PTDAAARGLGQHDLVRVFNDRGAVICAVRLTERVPAGTVHSYESSAVYDPVGEPGNSP
                     DRGGCMNLLTPSRMMIRRAHALAANSCLVQIESWDERA"
     gene            8633..9412
                     /locus_tag="VFZ86_08860"
     CDS             8633..9412
                     /locus_tag="VFZ86_08860"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX5642432.1"
                     /translation="MPRRRADDEGVDGGMQVITRAAAILGALTEPEGGGRSLAQLAAQ
                     TGLPKTTVHRICAALEHVGYVHTDAATGRRELGSSLLRLAVVGRRDLIAVLEPSLERL
                     SMDLNETVDLGVLDGGQVLFVAQHPAPERALMAIARVGAQFPAYTLASGKALLAQLPR
                     EDALRRLPRRLEPTLGGRPRTRETLLRELDEVRATGVAYEREEMRHGICAVAVAVTDV
                     DGRAASVAVPMPAARFAESEDHVAARLLALRDQTEARLGGA"
     gene            9423..10187
                     /locus_tag="VFZ86_08865"
     CDS             9423..10187
                     /locus_tag="VFZ86_08865"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5642433.1"
                     /translation="MDAFDFSGRRVLVVGAGGLGAAIAAGFAAHGAAVAAADVDGERA
                     AGLARELSAAHGVTTTGIRVDVTDETSVRAMVVAAVRELGGLDVLVNAAGVTVRTRAE
                     DLAPDDWRRILDVNATGTFLCCQAAGAQMARAGGGAIVNLSSVRGRYGATFGQAEYSA
                     SKGAVDALTRSLAAQWAEHGIRVNAVAPTFVETDLTRSVLADEQFAAGLRASIPMGRW
                     GETEDVVGPVLFFASPAARFVTGQILYVDGGLTARV"
BASE COUNT         1792 a         3712 c         3646 g         1488 t
ORIGIN      
        1 tgcgggacgg cgccccgact cgatggccgc cgcggcggtc cagttgaccg gccttcgatg
       61 acggtgcgag cattgcgacg gactggccgg cgcggggaga aaggcctgct cgacgacatg
      121 gcggacagcg cgacagacgg ggatcagcgg cgcatcggcg tgcaggtcat cgcgcgcgcc
      181 gccgagatct gccgcgagct ggcgacggcg acggatggcc tgacgacggc cgagctcgtc
      241 gaacggctcg gcctgtcccg tcctaccgtc taccgcatcc tgcgggcgct catggacgag
      301 gacttcgtgc gtcaggtcgc ggccggccgc tacgccatcg ggccggcctt cctcgtgatc
      361 ggcggttcgt cctacggggg cctgcggcac gacctgcgcc ccttcatgag ggacctctcc
      421 aacgagctgg gagagaccat cgacctcgcg gtgctggaca gcggcgaagc gctgttcgtg
      481 gaccagtacg tcgcggtgca agggctccgc gtggtcgccc acatgggggc acggctccct
      541 ctgtactgca cggcgagcgg caaggcgctg ctcgcggagt tgtcgcccgg cgaggccgaa
      601 cgcgagttac cggagacgct cgaggcctac accccgaaca ccgggaccga ccgggccgcc
      661 ctgctccgcg agctcgagga ggtgcgcagg accggggtcg cctacgacaa cgaggagtac
      721 gtgatgggcg tctgcgccgt agccgcgccc gtccgcgatg ccgtcggcgc agtcggggcg
      781 ttgacggtgg aggtcccggc gagccgcttc agcgggcgcg aagacacgct cacgcgcgcc
      841 gtgctgcggc agcgcgacct cgctcatcgc gccctggtcg gggcgggcca gacgaggcga
      901 gactagaccc tcctcccgct cacaccgcca tgcggatcgc ttgacgttcc tcccgaccga
      961 ctctagtctg tgtccgtaat gtgggcggta tgtccgcatt gtgagctacg acggaatggc
     1021 tgcggtcttg aggcaccggg tgcgccatca ccgacgcacg gcatcatcga cagcggggtg
     1081 tcacgccgcg acttcctgaa caaggcccgc cgacgggcgc ggcgccgggc gtgggcggcg
     1141 ccctcgcgag gccctgacag aggggcgaca ggtctacagg accaaggaga cggccaaatg
     1201 ggagaagcag tccgcctgac gaactcgtcg accggtggcc cggtcttcgt ctatgtggag
     1261 gacggcaaga tcgtccgcat cacccccatg gacctggacg acgccgtcga cgcggcctcg
     1321 tggaagatcg aggcgcgcgg caagaccttc cggcctccac gcaagacgac gtactcggtc
     1381 tacaccgccg gtttcaagtc catgatctac tccgaccgcc gcatcaagac cccgatgaag
     1441 cgcgtggact tcgacccgga cggagagcgc aacatccaga atcgcggcat ctccggctac
     1501 gagccgatct cgtgggacga ggccaccgac atcgtggtga gcgagatcaa ccgcgtgaag
     1561 cgcgaggccg gtcccgcggc gatcctctcc agccccagct cccaccacat gtggggcaac
     1621 gtcggctacc gccacagcac ctacttccgc ttcatgaaca tgatgggctt cacctacgcc
     1681 gaccataacc cggacagctg ggaaggctgg cactggggcg gcatgcacat gtggggcttc
     1741 agttggcgcc tcggcaaccc cgagcagtac gacctgctcg aggacacgct ggagaacacc
     1801 gagatgatcg tcttctggtc gtccgacccg gagaccaaca ccggcatcta ctccgcgcac
     1861 gagtccacgc cgcgccgctt ctggatgaag gacctgggca tcaagatggt cttcatcgac
     1921 ccgttctaca accacaccgc cgggctcatc ggcgacaagt ggttctctcc caagatcggc
     1981 accgaccacg cgctctcgtt cgccatcgcc cacacctggc tgaccgagga cacctacgac
     2041 aaggcgtacg tggccacgca cgcccacggc ttcgacgagt gggccgacta cgtgctcggc
     2101 aagaccgacg gcgtgcccaa gacctgcgag tgggccgagg ccgagtccgg cgtccccgcc
     2161 tgcgagatcc gcgccctcgc ccgcgagtgg gccaagaaga agaccatgct ggccgccggc
     2221 ggcctgggag gctggggcgg cgcctgccgc gcctcgcacg gcatcgagtg gagccgcggc
     2281 atgatcgccc tcgccaccat gcagggcatg ggcaagccgg gcagcaacat ctactcgacg
     2341 acccagggca cgcccgtcga ctacgacttc tacttccccg gctacgccga gggcggcatc
     2401 tccggcgact gcgagaactc cgccgcgggc ttcaagttcg cctggcggat gttcgacggc
     2461 aagaccacgt tcccgtcgcc gtcgaacatc aacacctccg ccggccagca catcccgcgc
     2521 ctcaaggtgc cggagtgcat catggacggc gagttccagt gggccggcaa gggcttcgcc
     2581 ggcggcgaca tccagcacca gctgcaccag taccggtatc cggctcccgg ctatcccagg
     2641 atcaagatgc tgtggaaata cggcgggccg tggatcggga cgatgacggc gaccaaccgc
     2701 tacgccagga tgtacgcgga cccgagcctc gagttcgtcg tcagccagtc catctggttc
     2761 gagggcgagg tgcccttcgc cgacatcatc ctcccggcct gcaccaactt cgagcgctgg
     2821 gacatcagcg agttcgccaa ctgctcaggc tacatccccg acacctacgg ccagtgcaac
     2881 caccgcgtga tctccttgca ggccaagtgc atcgagcctg tcggcgaatc caagtcggac
     2941 taccagatct acgcgatgat cgccgagaag ctcggcatcg ggccgatgtt caccgagggc
     3001 aaggacgagc tggcctgggt ggagcagtac tacaacgcca ccgacatgcc caagcacatg
     3061 agcttcgccg agttcaagga gaagggctac ttcatcgtgc cggacaaccc ggagcgcaag
     3121 aagacgccgg cgctgcgctg gttcgccgag gaccgcgaga aggacacgcc cgactggggc
     3181 ccgcgcctga acaaccaggt cggccgcaag ggcctgcaga cgagcaccgg caaggtcgag
     3241 ttcatcgcca ccagcctcaa gaacttcgag gaccagggct tcgtggacga gtaccgtccc
     3301 tcgatgcaca cgtacgtgcc cgcctgggaa ggcccggctt gcgagctcgc caggaagtac
     3361 ccgctcggga tgctgtcacc gcacccgcgc ttctcgttcc acaccatggg cgacggcaag
     3421 gacagcttca tgaacgacat caaggaccac cgcgtcctgg tggacggtca ctactactgg
     3481 atcatccgca tgaaccgcct cgacgcggcg gcccggggca tcaggagcgg cgacctcgtg
     3541 cgcgccttca acgaccgcgg ctcggtcatc gtctgcgcgc aggtcggcga acgaatgcag
     3601 ccgggcaccg tgcacaccta cgagtcgtgc gccgagtacg acccgctcgg caagcccggc
     3661 aggtccgccg accgcggcgg gtgcatgaac atcctcaccc ccgaccgcta catgtccaag
     3721 tacgcgtcgg gcatggctcc gaacactgcc cagatcgaag tggagaaatg ggacggcggc
     3781 atctacgaga ccgaggagtc ggtgcagtga agcaatggta caaagtcatc gacgtggccc
     3841 ggtgccacga ctgcaacaac tgcttcatgg ccgacaagga cgagttcgtc ggcaacgact
     3901 ggcccgggta caccgccgcc cagccccggc acggccaccg ctgggtggac atcctgcggc
     3961 gcgagcgcgg ccagtatgcg cgcaacgacg tggcgtacct cccggtgccg tgtcagcact
     4021 gcgagaacgc gccgtgtatc gacgcgtcgg acggcgccat cacgaagcgc gaagacggca
     4081 tcgtgatgat cgacatggtg aaggccaagg gcaacaaggc tttggcccag tcctgcccct
     4141 accacgccat ctggtggaac gaagaagccg acatcgccca gaagtgcatc ttctgcgcgc
     4201 atctgatcga cgatcccacc tggacgccgc acacgacccg ctgcacgcac agctgcccga
     4261 ccgacgccat ggcgacctac ttcgtcgaac cggaagagat ggcgcggatg atcgcggcgg
     4321 agaagctgga ggcctacctt ccggacctcg gcaccaagcc gcacaccctg tacaagaacc
     4381 tgttcaggtt caccctgaac ttcatcaccg cgggggtcct tgtcgacggc gactgctacg
     4441 agggcgcgac cgtcaccttg cgctcgcacg agggtgggat cgcctgcgac gccggcggtg
     4501 cgggcatggc cgacggcggc gacacggcga cgctcgcgag gcgccgcacc aacttcttcg
     4561 gggagttcaa gttcgacggg ctcgagtacg gcgagtacga gctcgaggtg gacgccggcg
     4621 gcaagcggta cgccgccagg gtcctcgtcg acaacgagtc gcagaacctg ggcttcatcg
     4681 agctctgagt cgagccgccg ttcgagagaa ggggcgagag ggagagccgc ggggccttcc
     4741 ctctcgcctc cagtcaggtg ggatcgcccg aggccgctcg cggagagagg acagcgcgtc
     4801 atgacgaaga tcgtggtgtt caacggcagc ccgcgcatgg acggcaacac gacgacgata
     4861 ctggaccttg tggccaaggg cgcccgggac cgtggggccg aggtcaagta ctacacgctg
     4921 ttcaagatga agttcatggc gtgccagagc tgcttcgcgt gccgcgtgaa gcaggattgt
     4981 gtcatcaacg acgagctgcg cgaggcgctg cacgaggtca cggccgcgga cgccgtcgtg
     5041 atcggctcgc ccgtctacat gatgcagatg accgggcccg tcaagaacct gtacgacagg
     5101 ctgtttcccc tgatggacgg agagggaagg ccgaggttcg gcgccaagaa gatgatcagc
     5161 gtgtactcgc agagcgtgga cgacgtgcac gcctacgact cctacttcga gtacaccgcg
     5221 gccatgtatc cgtcgttcgg cttcgagtgg gtggacaaca tcgtgtgcac caacgccaac
     5281 gaccccgaga gcgcggacca cgatgccgag ctgcagatcc gcgcctacga agcgggcagg
     5341 gccctcgcgc gctgagtgct ccgtcgggcg ctaccggcgg cgcgccatga tgcccccgtg
     5401 atccggggac gggcgcgcgg accggcgccc gttcgcatgt agcgaggcct ctcccgcccc
     5461 tcccccgtcc aacgattgac ccggcagcgg gcgttcgcta tcgtaccgtt atacggaata
     5521 gatattccgt ttaacggaac aagcgagtga cctatagtgg cactcgccgg gtgcggagga
     5581 ccgcatggct ggcacggacg ggcgccgggt ggcgcgggca gtcggacagg cgaaacacgc
     5641 cgcaggccgg gcgaagaaga cggccgccga ggcgcagttc acggcgacgc tggccggcct
     5701 cgaggtcgcg ttgcgcgcga cggccgggcg gtacccggcg ttcgccgcca ggctgcgcga
     5761 gcgcgacctg gtcgcccaga tccgcctcgc gggcagggcg cgggggcgcg cctacaccat
     5821 caagggcggc agggtgcgct cgcgtgcggg gctgcaccct gcgcccgacc tcaccatgac
     5881 cttcgactcg gcggaggtcg cggcccgcat catgcggccg ggccgcgact acctcgagtt
     5941 catcgacgcg ctcaagaact tccagatggt ggtcgagggc ccggacgagc tggccgtctg
     6001 gttctccgag acgctgcaga tgatgctcac cgccggcctc gagttcggcg tggacgcggg
     6061 cgctggcgtc cgccgcttca ccagcaacac caacggcggg cccgtcttcg tctacgtgcg
     6121 ggacgggcgc atcctgcgca tcacccccat cgagttcgac gaggacgacg ccgaggcgtg
     6181 gacgatcgag gcgcgcgggc ggtcgttcac gcccccccgc aagacgacgg tcagctcgca
     6241 cacgctcgcc tggaagtcgc tcatctactc gccggaccgc ctgctccacc ccatgaagcg
     6301 cgtggacttc gatccggacg gcgagcgcaa cccgcagaac cgcggcacgt ccggctacga
     6361 gcgcatcagc tgggacgagg cgctcgacct ggtggcctcc gagatcaagc gggtcaagcg
     6421 cgaccacggc ccgggcgcga tcatgaacgg cagcggctcg caccacacct ggggcgtgct
     6481 gggctactgg ctgagcgccc gcatccgctt cttcaacatg ctcggctggt cgccggtgat
     6541 gcacaacccg gacagctggg agggctggta ctggggcgcc gcgcaccact ggggccagag
     6601 cgcgcgcaac ggcggcggcg agacctacgg caccgtggag gacctgctca agcacgccga
     6661 gatggtcgtc ttctggtctg ccgacccgga ggccaccagc ggcgtctacg gcgcgcacga
     6721 cggcaccatc cgccgccagt ggctcaagga cctcgacatc ccctgcgtgc acatcgaccc
     6781 gttccacaac cacacggccc agttgctcgg cggcaagtgg atcgccccgc gcccgggcac
     6841 ggacagcgcc ctggtgctcg ccatcgctca tgtctggatc accgaggggc tgtacgacgc
     6901 cggctacgtc gccgagcgca ccgtcggctt cgagaagtgg cgggcctaca tcctcggtga
     6961 ggaggacggg gcggggaaga cgcccgagtg gcaggaggcc gagacgggcg tgccggcgcg
     7021 cgaggtgcgc gcgctcgcgc gcgcctgggg gaagaagaag acgtacctcg ccgccggcgg
     7081 cctgatcggc ttcggcggcg cttgccgcac ggcgaccggc agcgactggg cgcgcggcgt
     7141 ggtctgcctc atggccatgc aaggtctcgg caagcccggc gtcaacatgg gctgcctgca
     7201 gcagggcacg ccgctcgaca cgcacttctt cttccccggc tacgcggagg gcggctactc
     7261 gggcgacctg tacggcaccg ccctcagcat caacatgtac cagcgcatgc cgcagctcgc
     7321 caccgtgaac accgtctccc aggtcgtgcc gcggctcaag atcccggagg ccgtcatgga
     7381 cggccactgc gaggcctatc ccaccgaccc caagtccatc gagggccagt tccagcgcta
     7441 cgagtacccg gcgccgggtc acagccggat caagatgtac tacaagtacg gcggctccca
     7501 cttcggcacc atgaccgaca ccaaccgctt cgcgcgcatg taccgctcgg acaagctgga
     7561 gttcgtggtc aatcagggca tctggatgga aggggagacg aagttcgccg acgtcatcct
     7621 cccggcctgc accaacttcg agcgctggga catcagcgag ttcgccaact gcggcggcta
     7681 catccagcac gcctacacgc agtgcaatca ccgcgtcgcg gtgatgcagc acaagtgcat
     7741 cgagccgctc ggcgagtcgc gctccgacca ccagatcttc ctcgacctcg cgacacggct
     7801 cgggctcggg tcgatgtact cggagggcgt caccgagctg gactggtgca agcggctgtt
     7861 cgacgccacc gacctgcccc gttccgtctc gtggaaggac ttcctcaaga agggctacta
     7921 cgtcatcccg ccgccgccgg aggagcggcg cgacccggtg tcgtaccgct ggttcgcgga
     7981 gggtcgcgcc aaggacacgc ccgagctgac gcccctgccg gccgactaca ccgaggagtt
     8041 cggcatgggc ctgcagacgc agtcgggaaa gctcgagttc gaggcctcga gcctcaagcg
     8101 cttcgacccc gacgacccgg agcgggcgcc catcatgcgc tacgtcccgg cgtgggaggg
     8161 cccgcacgcc gccgagctct acgggaagta cccgctgcag ctcatcacgc cgcacccgcg
     8221 gcacagcttc cacacccaga gcgacggcaa ggacgcggtg gtgaacgacg tccgcgacca
     8281 ccgcgtcctc gtcgacggcc gctactactg gatcgtgcgc gtgaatccca cggacgcggc
     8341 cgcgcgcggc ctcgggcagc acgacctggt ccgcgtgttc aacgatcgcg gcgccgtgat
     8401 ctgcgcggtc cggctgacgg aacgcgtccc ggcaggtaca gtgcactcgt acgagtcgtc
     8461 cgccgtctac gatcccgtgg gcgagccggg gaactccccc gaccggggcg gctgcatgaa
     8521 cctgctcacg ccgagccgca tgatgatccg cagggcgcac gccctggcgg ccaactcgtg
     8581 tctcgtgcag atcgagtcgt gggacgagcg ggcctgacgc gagaggggac acatgccgag
     8641 gcggcgggca gacgacgagg gcgtggacgg cgggatgcag gtgatcacgc gggcggcggc
     8701 gatcctgggc gccctgaccg agcctgaggg cggcggccgc agcctcgctc agctcgcggc
     8761 ccagacgggg ctgcccaaga cgaccgtgca ccgcatctgc gcggcgctcg agcacgtcgg
     8821 ctacgtgcac accgacgcgg ccacggggcg gcgggagctc ggctccagcc tgctgcgcct
     8881 ggcggtggtg ggccgccgcg atctgatcgc ggtgctcgag ccctcgctgg agcgcctgtc
     8941 gatggacttg aacgagaccg tggacctggg cgtgctcgac ggcggccagg tcctgttcgt
     9001 ggcccagcat ccggcgccgg agcgcgcgct catggcgatc gcgcgcgtcg gcgcgcagtt
     9061 ccccgcctac accctggcga gcggcaaggc gctgctggcg cagctgccgc gcgaggacgc
     9121 actgcgccgc ctgccacggc ggctggaacc caccctcggc gggcgcccgc gcacgcgcga
     9181 gacgctgctg cgcgagctcg acgaggtgcg cgccaccggg gtcgcgtacg agcgcgagga
     9241 gatgcgccac ggcatctgcg ccgtggccgt ggcggtcacc gacgtcgacg gccgcgcggc
     9301 atccgtcgcg gtgccgatgc cggcggcccg gttcgcggag agcgaagacc acgtggcggc
     9361 gcggctgctg gcgctgcgcg accagaccga ggcccggctg ggcggagcct gaggaggcgg
     9421 ggatggacgc gttcgacttc tcgggccggc gcgtgctcgt ggtgggcgcc ggcggcctcg
     9481 gcgccgcgat cgccgccggg ttcgcggcgc acggcgccgc ggtcgccgcc gccgacgtcg
     9541 acggcgagcg ggccgccggg ctcgcccgag agctctcggc ggcgcacggc gtcaccacca
     9601 ccggcatccg ggtggacgtc accgacgaga cctccgtgcg cgcgatggtc gtggccgccg
     9661 tgcgtgagct cggcggcctg gacgtcctcg tcaatgccgc cggcgtcacg gtgcgcacgc
     9721 gcgccgagga tctcgcgccg gacgactggc ggcgcatcct cgacgtcaac gccaccggca
     9781 cgttcctctg ctgccaggcc gccggcgctc agatggcgcg ggccggcggc ggcgcgatcg
     9841 tcaacctctc gtcggtgcgc ggccgctacg gcgccacctt cggccaggcg gagtactccg
     9901 ccagcaaggg cgccgtggac gcgctcacgc ggtcgctcgc cgcccagtgg gcggagcacg
     9961 gcatccgcgt caacgccgtg gcgccgacct tcgtcgagac cgacctcacg cgctcggtcc
    10021 tggccgacga gcagttcgcg gccgggttgc gtgccagcat cccgatgggc cgctggggcg
    10081 agacggagga cgtcgttggc ccggtcctgt tcttcgcctc accggcggcg cggttcgtga
    10141 ccggccagat cctgtacgtg gacggcggtc tcacggcgcg cgtctagctc tttccgcgca
    10201 gcaggatcct cggtccggta cgcggccgag ccgcgcctcg gcggcccttg cgcacgcacg
    10261 gcgccgatgc ttgacctgcc cgaacccctg cactagtgtt cgcatcatgg actagttgtt
    10321 ccgcatcgtg ggcactcgct cgcgacggga acaggcgtga ggggggcccg gtggagggtc
    10381 ggtccggcag cggcagagcg tcagatcgcg ccgcaggacc agcgcacgac cgcagcgggc
    10441 cggacacgag gcgagaggag cactgcgatg gctgagcgtg agatctgggg tgtcgacaag
    10501 atccgcaacc tgttcgatct gagcggcaag acggcgatcg tcaccggcgg ctccggagcc
    10561 ttcggccacg acgccgccaa gggcctcgcg gccttcggcg ccaacgtcgt ggtcaccagc
    10621 cgtcgcgccg agaccctc
//
LOCUS       DASUMX010000179         8998 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_638898, whole genome shotgun sequence.
ACCESSION   DASUMX010000179 DASUMX010000000
VERSION     DASUMX010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 8998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8998
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_638898"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..918
                     /locus_tag="VFZ86_08870"
     CDS             <1..918
                     /locus_tag="VFZ86_08870"
                     /inference="COORDINATES: protein motif:HMM:NF039585.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HEX5642434.1"
                     /translation="VTVAKLLAPYTPFVAEEMYQNLVRSADPEAPESVHLCAWPAADE
                     QAIDAGVSFDMAAARRVVEMGRAARNAAAVKTRQPLAEVVVALPEAERRAVERLQDVV
                     LDELNVKDLRLVGGEEELVAYVVKPNLKVLGPKLGKRLGPLQAALKEADAAALVAAVR
                     ADGAAVIALPDGEISLAEDELLIETGSPEGYQVESEAGRVVALKTHVDDALREEGLAR
                     ELIHAVQLARKAADLRIEDTISLTLDVPEELRPPAERHRGAIMAETLASALALGEAAG
                     DHRETARVDGHEVGIGISVTGTIFTVTYG"
     gene            1014..1703
                     /locus_tag="VFZ86_08875"
     CDS             1014..1703
                     /locus_tag="VFZ86_08875"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5642435.1"
                     /translation="MRDPSLDLLVDLARPTSDDRVLDYATGAGMAGFIVAPDVATVEA
                     ADEQPDMLEEGMRLSAELGLVNVAFTLVDLHALPYKDGTFSLVVSRNAFHLLPDPGTA
                     LAELGRVLAPGGRVVILDAVVDETTDTAFNEIARLREPAHRRHYRPDELDTIVAGAGF
                     SVAGRGSVRSTIDLAYWLQAAAVPPPKAALIRGRFKALPVEVQMGMDVAFSDKSVSFS
                     YDVAGLRLERV"
     gene            complement(1947..2261)
                     /gene="trxA"
                     /locus_tag="VFZ86_08880"
     CDS             complement(1947..2261)
                     /gene="trxA"
                     /locus_tag="VFZ86_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX5642436.1"
                     /translation="MDAVSQDNFEAEVIKAPGKVLVDFWAEWCGPCRMIGPVLDEMAG
                     DYPDVKFVKLNVDEAPAVAQQFSVMNIPTILAFEAGEAKHRIVGAMPKAKLVEELGAF
                     LG"
     gene            complement(2326..3345)
                     /gene="trxB"
                     /locus_tag="VFZ86_08885"
     CDS             complement(2326..3345)
                     /gene="trxB"
                     /locus_tag="VFZ86_08885"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEX5642437.1"
                     /translation="MSDPSSDTGAEVYDVVIVGAGPAGLTAAIYASRGRLKTAVLERN
                     MAGGQIALTDLVENYPGFPEGISGFDLSQKMKEQAEKFGAEMREIEGVAELRSDPEGC
                     WIVATDREQIRTRAVILAPGVEPRRSGIPGEAEFIGRGVSWCATCDGALYRGKTVAVI
                     GGGDAAVEEGMFLTKFAEKVYLVHRRDELRAAPIAQERAFANPKFEFVWDSIPKQIDG
                     AEMVEALEVENVKTGEGRSLPVNGVFMYIGQIPNTAWLKDTVELDEYGHIVTDELLRT
                     KLPGVFACGDARANPLKQIAMAVGEGALAAVQVGHYLDELDSAPGTTTAAGESAAAPP
                     ATTQR"
     gene            complement(3445..4473)
                     /locus_tag="VFZ86_08890"
     CDS             complement(3445..4473)
                     /locus_tag="VFZ86_08890"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014321358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEX5642438.1"
                     /translation="MAAGYTVAIAGATGVVGGTVRAILEERDFPIGRLRLLASTRSVG
                     RKLPYRGEDVEVQELGPGSFAGVDLVFFAAGGSVSKEFVPQAVAAGATVIDKSSMFRQ
                     DPGVPLVVPEVNADHLAAHTGIIATPNCSTIQMVVALKPIYDAAGIERVVVSTYQAVS
                     GSGQGGIAALAAQARQWAAGEPVEQQFYPHQIAFNVLPHIDGFLPTGSTKEEQKMVDE
                     TVKIFADPDLKVTATCVRVPVFGAHSEAVNVQTKRKLSAAEAREILAAAPGVVVVDDP
                     AELQYPMPLPAEGEDPVYVGRIREDATIANGLDLWVVADNLRKGAALNAVQIAEELVA
                     RDLLRARA"
     gene            complement(4473..5744)
                     /locus_tag="VFZ86_08895"
     CDS             complement(4473..5744)
                     /locus_tag="VFZ86_08895"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEX5642439.1"
                     /translation="MSVVVQKYGGTSVADAARIGNVARRIVATFEQGNSVCAVVSARG
                     DTTDELLELAREITPNPPEREMDMLLSAGERISCALLAMAIHRLGYDAVSLTGSQAGI
                     VTDTVHMKARILDIHPARVKDAMDDGKIVLVAGFQGVSTEKNVTTLGRGGSDTTAVAL
                     AHQLDAAVCEIYTDVDGVYTANPSLVAKARKIDAISYDEMLEMAASGAQVLALRSVEY
                     ARKYDIPIHVRSSFTEAEGTWVTDGGHRMDTEQQLEQPIIRAVSYTTDEAKVTLRGVP
                     DRPGVAARVFTALAKGHVNVDTIIQNTSESGHSDISCTIPAEDIPAADKALKEIVKEI
                     GAREYDTDAEIAKISLIGAGMRSHPGVAAKMFQTLADLGINLDMIGTSPIKISCVIAK
                     EHVDEAVRSLHTVFDLDEDAVSHEPSDRGSC"
     gene            complement(5769..6500)
                     /gene="nadE"
                     /locus_tag="VFZ86_08900"
     CDS             complement(5769..6500)
                     /gene="nadE"
                     /locus_tag="VFZ86_08900"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HEX5642440.1"
                     /translation="MEALARHLTDWIAAEVAAAGGSGAVFGLSGGIDSAVVAALAKRA
                     FPHHTLGVLMPCHSDPQDAEDAAIVAHHFDVPSCTVDLAPVYDLLLEQLAVSCTDMPD
                     SRLATANLKPRLRMTTLYAFANQLDYRVLGTGNRSELAVGYFTKYGDGGADFLPLGDL
                     VKGEVRDLARHLGVPERIVAKPPSAGLWADQTDEAEMGLTYEELDACLLTSEASPAVK
                     AKVDAMHAASEHKRALPRIAPKPGA"
     gene            complement(6596..7930)
                     /locus_tag="VFZ86_08905"
     CDS             complement(6596..7930)
                     /locus_tag="VFZ86_08905"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011309506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEX5642441.1"
                     /translation="MTMTDQRQAAIEKLVKDENVKHIQLWFTDILGNLKMVEIPDRQL
                     PNVIESGAAFDGSSISGYAEIEESDIVAMPDWDTFKVLPWSQGSERTAFVFCDVLDRN
                     YKPFEGDPRWVLRRQLERAKKLGLDFYVGPELEYFYFKDSVKPELTDDGGYFDVLPAD
                     LGNDLRKQTMRTLDKLDIPMEASHHEVAPSQHEIDPHYDKALLMADRVMIIRLIVKEV
                     AAHNGVHATFMPKPMHGENGSGMHVHQSLFRGDSNAMFSETDSDHLSDIAKGFIAGQL
                     KHMREFTSILNQYVNSYKRLVPGYEAPVYISWAHRNRTALIRVPLYVQGKESTVRAEL
                     RNPDPGANPYLAFAVMLGAGLDGIEKGYELPPGVEPNIYKMTAGEREDIGLGALPNNL
                     YEAIQHTQDSEMVRRVLGDHVFERFVTNKLNEWYEYREQVTDWEIRKYFSKI"
     gene            8130..>8998
                     /locus_tag="VFZ86_08910"
     CDS             8130..>8998
                     /locus_tag="VFZ86_08910"
                     /inference="COORDINATES: protein motif:HMM:NF012531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit alpha"
                     /protein_id="HEX5642442.1"
                     /translation="MSKRRSAIDLPGAAVNGARRAHPPRSDGAVAAWHAGGRDAGGRD
                     HSACGVGLVARISGEPDREVVELALGALAAMEHRGGVGADGEASDGAGVLTAIPWRVL
                     EPWLANQRIAEPRPFSTAVGMVFLPREQGKRTLARELVNKALRDEGLVVVGWREVPLR
                     PEALPPSVRERHPHVEQVVAQSPMLAGDELERALLLARRAVGRALIRQPVIRRLEDLY
                     ICSLSSRTVVYKGMVTAPVLPHFYPDLQDPGFVSQFAVLHRRFSTNTAPKWSLAQPMR
                     LLAHNGEINTLLG"
BASE COUNT         1217 a         3033 c         3292 g         1456 t
ORIGIN      
        1 gtcaccgtgg cgaagctgct cgcgccctac acgccgttcg tcgccgagga gatgtaccag
       61 aacctggtca ggtccgctga cccggaggcg cccgagagcg tgcacctctg cgcgtggccg
      121 gccgccgacg agcaggcgat cgacgccggc gtgagcttcg acatggcggc tgcgcggcgc
      181 gtcgtcgaga tgggccgcgc ggcgcgcaac gcggccgccg tcaagacgcg ccagccgctg
      241 gccgaggtcg tcgtggcgct gcccgaggcc gagcgccgcg cggtcgagcg cctgcaggac
      301 gtcgtcctcg acgagctcaa cgtcaaggac ctgcgcctcg tgggcggcga ggaggagctc
      361 gtcgcctacg tcgtcaaacc caacctcaag gtgctcggcc ccaagctggg caagcgcctg
      421 gggccgctgc aggcggcgct caaagaggcg gacgcggcgg cgctggtcgc cgccgtgcgc
      481 gccgacggcg ccgccgtcat cgccctgccg gacggcgaga tcagtctcgc cgaggacgag
      541 ctcctcatcg agaccggctc gcccgagggc taccaggtgg agagcgaagc cggccgcgtc
      601 gtcgcgctca agacgcacgt cgacgacgcg ctgcgcgagg agggactcgc gcgcgagctc
      661 atccacgcgg ttcagctcgc gcgcaaagct gccgacctac gtatcgagga caccatcagc
      721 ctgacgctgg acgtgccgga ggagcttcgc ccgccggccg agcgccaccg cggcgccatc
      781 atggccgaga cgctggccag cgcgctggca ctcggcgagg cggccggcga ccaccgcgag
      841 accgccaggg tcgacggcca cgaggtcggc atcggtatca gcgtgaccgg caccatcttc
      901 accgtcacct acggctgacg ggggggaccg gccggcggcc agccggccgc ggacccggcc
      961 ggcgacccgt gctcgacggg cggccggcga cgaagggagg agccgtggaa cagatgcgtg
     1021 acccgtcgct cgacctgctc gtcgacctcg cccggcccac gtccgacgac cgagtgctcg
     1081 actacgcgac gggcgccggc atggccgggt tcatcgttgc cccggacgtg gcgaccgtgg
     1141 aggccgccga cgagcagccc gacatgctcg aggagggcat gcggctctcc gccgagctcg
     1201 gcctcgtcaa cgtcgccttc acgctggtcg acctccacgc gcttccgtac aaggacggca
     1261 cgttcagcct cgtcgtctcc cgcaacgcct tccacctgct gcccgacccc ggcaccgcgc
     1321 tggccgagct ggggcgcgtg ctcgcgcccg ggggacgcgt cgtcatcctc gacgccgtgg
     1381 tggacgagac caccgacacg gctttcaacg agatcgcacg cctccgtgag ccggcccacc
     1441 gccggcacta ccgccccgac gagctggata ccatcgtggc cggcgccggg ttttccgtgg
     1501 ccggccgggg gagcgtgcgc agcaccatcg acctcgcata ctggctgcag gcggccgcgg
     1561 tgccgccgcc gaaggcggcg ctcatccgcg gccgcttcaa ggcgcttccg gtcgaggtcc
     1621 agatgggcat ggacgtcgcc ttctcggaca agtccgtgag cttcagctac gacgtggccg
     1681 gcctccggct cgagcgcgtc tgagcgctcc ccagcgggcc tgaacctccc gagcggcctg
     1741 aacgccgccc ggcgcggacc cgctcgaccc cgccgggctc gtccggcgcg aggtgatgtg
     1801 cgaacgccct gctacgcttg gccgtacgct tgggagtaca gcggtacatc cgcgcgtaca
     1861 tccgggcgtg cgactccctg cggcactaca cctgcccggg tcctgcagtg tgcttggacg
     1921 ccgggggtcg cgcccgctcg ggtgtatcag cccaggaagg cgccgagctc ctcgacgagc
     1981 ttggccttgg gcatcgcgcc gacgatgcgg tgcttggcct cgccggcctc gaaggcgagg
     2041 atcgtgggga tgttcatgac gctgaactgc tgcgccacgg ccggcgcctc gtccacgttg
     2101 agcttcacga acttgacgtc ggggtagtcg cccgccatct cgtcgaggac cgggccgatc
     2161 atgcggcagg ggccgcacca ctcggcccag aaatcgacca ggaccttgcc gggagccttg
     2221 atgacctcag cctcgaagtt gtcctgtgag acagcgtcca cctacatcct cctgtgagtc
     2281 gttgtgagcc ggcgcgcatc agcgtagcgc gccggcgcgc gttcgtcagc gttgcgtggt
     2341 cgcggggggt gcggcggcgc tctcgccggc cgccgtggtg gtccccggcg cggaatcgag
     2401 ctcgtcgaga tagtgcccga cctgcacggc cgcaagcgct ccctcgccga cggccatggc
     2461 gatctgcttc agcgggttgg cgcgggcgtc gccgcaggcg aacacgcccg gcagcttcgt
     2521 gcgcagcagt tcgtcggtga cgatgtggcc gtactcgtcc agctcgacgg tgtccttgag
     2581 ccacgccgtg ttcgggatct ggccgatgta catgaagacg ccgttgaccg ggaggctgcg
     2641 cccctcgccc gtcttgacgt tctccacctc gagggcctcg accatctcgg cgccgtcgat
     2701 ctgcttgggg atggagtccc agacgaactc gaacttggga ttggcgaacg cgcgctcctg
     2761 ggcgatgggg gcggcgcgga gctcgtcgcg gcgatggacc aggtacacct tctcggcgaa
     2821 cttggtgagg aacatgccct cctcgaccgc ggcgtcgccg ccgccgatca cggcgacggt
     2881 cttgccgcgg tagagggcgc cgtcgcaggt ggcgcaccag ctgacgccgc ggccgatgaa
     2941 ctcggcctcg ccggggatgc ccgagcgccg cggctccacg cccggcgcga ggatgaccgc
     3001 gcgcgtgcgg atctgctcgc ggtcggtggc cacgatccag cagccctcgg ggtcgctgcg
     3061 gagctcggcc acgccctcga tctcgcgcat ctcggcgccg aacttctccg cctgctcctt
     3121 catcttctgc gagaggtcga agcccgagat gccctcgggg aagccggggt agttctcgac
     3181 gaggtcggtg agcgcgatct ggccgccggc catgttgcgc tcgaggacgg ccgtcttgag
     3241 gcggccgcgg ctggcgtaga tggccgccgt gagccccgcg gggccggcgc cgacgatgac
     3301 gacgtcgtac acttcggcgc cggtgtcact gctgggatca gacatgtcgt tcctcgtggt
     3361 gggcgggctg gtcagtggat gcgcgtgagt atacccgccg ggaacgggcg cagtcggaga
     3421 ttccgggcac ggcggccggc cggctcaggc ccgggcccgg agcagatccc gggccacgag
     3481 ctcctcggcg atctgcaccg cgttgagcgc cgcgcccttg cgcaggttgt ccgccacgac
     3541 ccacaggtcg aggccgttgg cgatggtcgc gtcctcgcgg atgcggccga cgtacacggg
     3601 gtcctcaccc tcggccggca gcggcatggg gtactgcagc tccgccgggt cgtcgacgac
     3661 caccacgccc ggcgccgcgg cgaggatctc gcgcgcctcc gcggcgctca gcttgcgctt
     3721 cgtctggacg ttgacggctt cgctgtgcgc gccgaacacg ggcacgcgca cgcaggtggc
     3781 cgtgaccttg aggtccgggt cggcgaagat cttgacggtc tcgtcgacca tcttctgctc
     3841 ctctttggtg ctgccggtgg gcaggaagcc gtcgatgtgc ggcagcacgt tgaaggcgat
     3901 ctggtggggg tagaactgct gctcgacggg ctcgcccgcc gcccactgcc gcgcctgtgc
     3961 ggcgagcgcg gcgatgccgc cctggccgct gccggagacc gcctggtagg tgctgacgac
     4021 gacgcgctcg atgccggcgg cgtcgtagat gggcttgagc gcgacgacca tctggatggt
     4081 ggagcagttg ggcgtcgcga tgatgccggt gtgcgccgcc aggtgatcgg cgttgacctc
     4141 gggcaccacc agcggcacgc cggggtcctg gcggaacatg ctcgacttgt cgatcacggt
     4201 ggcgccggcc gcgaccgcct ggggcacgaa ctccttgctc acggacccgc cggcggcgaa
     4261 gaagacgagg tcgacgccgg cgaacgagcc agggccgagc tcctgcacct cgacgtcctc
     4321 gccccggtac ggcagcttgc ggccgaccga gcgcgtgctc gcgaggagcc gcagtcttcc
     4381 gatggggaag tcgcgctcct cgaggatcgc gcgcacggtg ccgccgacga cgccggtcgc
     4441 gccggcgatg gcgacggtgt acccggcggc catcagcagc tccccctgtc ggacggctcg
     4501 tggctcaccg cgtcctcgtc gaggtcgaac acggtgtgca gcgagcgcac ggcctcgtcg
     4561 acgtgctcct tggcgatgac gcaggagatc ttgatgggcg aggtgccgat catgtccagg
     4621 ttgatgccga gatcggccag cgtctggaac atcttggcgg cgacgccggg gtggctgcgc
     4681 atgccggcgc cgatcaggct gatcttggcg atctcggcgt cggtgtcgta ctcgcgcgcg
     4741 ccgatctcct tgacgatctc cttgagcgcc ttgtcggcgg cggggatgtc ctcggccggg
     4801 atggtgcacg agatgtcgct gtggccgctc tcgctggtgt tctggatgat ggtgtcgacg
     4861 ttgacgtggc ccttcgcgag tgcggtgaag acgcgcgccg cgacgcccgg ccggtccggg
     4921 acgccgcgca gcgtgacctt ggcctcgtcg gtggtgtagc tgacggccct gatgatcggc
     4981 tgttccagct gctgctcggt gtccatgcgg tggcctccgt cggtgaccca cgtgccctcg
     5041 gcctcggtga aactggagcg cacgtggatg gggatgtcgt acttgcgcgc gtactcgacc
     5101 gagcgcaggg cgagcacctg cgcgccgctg gccgccatct cgagcatctc gtcgtaggag
     5161 atggcgtcga tcttgcgtgc cttggcgacc aggctgggat tggcggtgta gacgccgtcg
     5221 acgtcggtgt agatctcgca gaccgccgcg tcgagctggt gcgcgagcgc cacggcggtc
     5281 gtgtcggagc cgccgcgccc cagggtggtg acgttcttct ccgtggacac gccctggaaa
     5341 ccggcgacga ggacgatctt gccgtcgtcc atggcgtcct tgacgcgcgc agggtggatg
     5401 tcgaggatgc gcgccttcat gtgcaccgtg tcggtgacga tgcccgcctg cgagccggtg
     5461 aggctcacgg cgtcgtagcc gaggcggtgg atcgccatgg cgagcagggc gcagctgatg
     5521 cgctcgccgg cggagaggag catgtccatc tcgcgctcgg gcgggttggg ggtgatctcg
     5581 cgggccagct cgagcagctc gtcggtggtg tcgccacggg cgctcacgac cgcgcagacg
     5641 ctgttgccct gctcgaacgt ggcgacgatg cgccgcgcca cgttgccgat gcgggcggcg
     5701 tcggcgaccg acgtgccacc gtacttctgg acgacgacag acaaggtgaa cctccgaaaa
     5761 aggcgggctt aggcgccggg cttgggggcg atgcggggga gcgcgcgctt gtgctcgctg
     5821 gcggcgtgca tggcgtcgac cttcgccttg acggcgggcg acgcctcgga cgtcagcaag
     5881 caggcgtcga gctcctcgta ggtgaggccc atctcggcct cgtcggtctg gtcggcccac
     5941 aggccggccg acggcggctt ggcgacgatg cgctcgggga cgcccaggtg gcgggcgagg
     6001 tcgcggacct cgcccttgac caggtcgccg agcgggagga agtcggcgcc gccgtcgccg
     6061 tacttggtga agtagccgac ggccagctcg ctgcggttgc cggtgcccag cacgcggtag
     6121 tcgagctggt tggcgaacgc gtacaacgtg gtcatgcgca gccgcggctt gaggttggcg
     6181 gtggccaagc ggctgtccgg catgtccgta cagctgacgg cgagctgctc gagcagcagg
     6241 tcgtagaccg gcgcgaggtc gacggtgcag gacggcacgt cgaagtggtg cgcgacgatg
     6301 gccgcgtcct cggcgtcctg gggatcgctg tggcagggca tgaggacgcc cagggtgtgg
     6361 tgcgggaacg cgcgcttggc gagcgccgcg acgacggccg agtcgatgcc gccggagagg
     6421 ccgaagaccg cgccgctgcc gccggccgcg gcgacctcgg cggcgatcca gtcggtgaga
     6481 tgtcgggcga gagcttccat gcgtcgcttt cggaagggca agtctaccgt acggcgaacg
     6541 ggccgggagg cgctgcgccc cccggcccgt ccggtcccct cgcgaggcgc gagcctcaga
     6601 tcttggagaa gtacttgcgg atctcccagt cggtgacctg ctcgcggtac tcgtaccact
     6661 cgttgagctt gttcgtcacg aagcgctcga acacgtggtc gccgaggacg cggcggacca
     6721 tctcgctgtc ctgggtgtgc tggatcgcct cgtacaggtt gttgggcagg gcgccgaggc
     6781 cgatgtcctc gcgctcgccg gcggtcatct tgtagatgtt gggctcgact cccggcggca
     6841 gctcgtagcc cttctcgatg ccgtcgaggc cggcgccgag catgacggcg aaggcgaggt
     6901 acgggttcgc gcccgggtcc gggttacgca gttccgcccg gacggtgctc tccttgccct
     6961 gcacgtacag cggcacgcgg atgagtgcgg tgcggttgcg gtgcgcccag ctgatgtaca
     7021 ccggggcctc gtagccgggc accaggcgct tgtagctgtt cacgtactgg ttgaggatcg
     7081 aggtgaactc gcgcatgtgc ttgagctggc cggcgatgaa gcccttggcg atgtcgctga
     7141 ggtgatccga atcggtctcg ctgaacatcg cgttggagtc gccgcggaag aggctctggt
     7201 gcacgtgcat gccgctgccg ttctcgccgt gcatgggctt gggcatgaag gtcgcgtgga
     7261 cgccgttgtg ggccgcgacc tccttgacga tgaggcggat gatcatgacc cggtcggcca
     7321 tgaggagcgc cttgtcgtag tgcgggtcga tctcgtgctg cgacggggcg acctcgtggt
     7381 ggctcgcctc catggggatg tcaagcttgt cgagcgtgcg catggtctgc ttgcgcaggt
     7441 cgttgccgag gtcggcgggc agcacgtcga agtagccgcc gtcgtcggtg agttcgggct
     7501 tgaccgagtc cttgaagtag aagtactcga gctcgggacc gacgtagaag tcgaggccca
     7561 gcttcttggc gcgctcgagc tggcggcgga gcacccagcg ggggtcgccc tcgaacggct
     7621 tgtagttgcg gtcgaggacg tcgcagaaga cgaacgcggt acgctcgctg ccctgggacc
     7681 acgggaggac cttgaaggtg tcccagtcgg gcatcgcgac gatgtcgctc tcctcgatct
     7741 cggcgtagcc gctgatggaa gagccgtcga aggcagcgcc gctctcgatc acgttgggga
     7801 gctgccggtc ggggatctcg accatcttga gattccccag gatgtccgtg aaccacaatt
     7861 ggatgtgctt cacgttctcg tctttgacga gcttctcgat tgcggcctgg cgctgatcag
     7921 tcatagtcat cctttcaggc caatcgggtt tatggcggcg ggtggcgccg tatggacccg
     7981 gcgattctga ccggtcggcg gcgcggattc aagtgagggg tgctgcaagt cacgcagcgc
     8041 ccccctcgct gacccagctt cgcaggtcag tcgcggagcg ggcgccggtg ggctacaatg
     8101 tcccaacgtc acccgcccgg taggactgct tgtctaaacg tcgctctgcg atcgacctcc
     8161 cgggtgcggc ggtcaacggg gcccgccgcg ctcaccctcc acgttccgat ggggccgtcg
     8221 cggcgtggca cgccggcggg cgcgacgccg gcgggcgcga ccactccgcc tgcggcgtcg
     8281 gtctggtcgc gcgtatctcg ggcgagccgg accgcgaagt ggtcgagctc gccctcggcg
     8341 ccctggccgc gatggagcat cgcggcggcg tcggcgccga cggcgaagcc tcggacggcg
     8401 ccggcgtcct gacggcgatc ccctggcgcg tgctcgagcc gtggctcgcc aaccagcgca
     8461 tcgccgagcc gcggccgttc tcgaccgcgg tcggcatggt gttcctgccg cgggagcagg
     8521 gcaagcgcac cctcgcccgg gagctcgtga acaaggcgct gcgcgacgag ggcctcgtcg
     8581 tcgtgggctg gcgtgaggtg ccgctgcgcc cggaggcgct gcccccgagc gtgcgcgagc
     8641 gccacccgca cgtggagcag gtcgtggcgc agtcgccgat gctggccggc gacgagctgg
     8701 agcgcgccct gctcctggcc cgcagggccg tcggcagggc gctcatccgc cagcccgtga
     8761 tccgccgcct ggaggacctc tacatctgct cgctctcctc gcggaccgtg gtctacaagg
     8821 gcatggtcac ggcgccggtg ctgccccatt tctatcccga cctgcaggat cccggcttcg
     8881 tcagccagtt cgccgtgctg caccgccgtt tcagcaccaa cacggcgccc aagtggagcc
     8941 tggcgcagcc gatgcgcctc ctggcgcaca acggtgagat caacacgctt ctgggcaa
//
LOCUS       DASUMX010000180         6606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_206821, whole genome shotgun sequence.
ACCESSION   DASUMX010000180 DASUMX010000000
VERSION     DASUMX010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 6606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6606
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206821"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1138
                     /locus_tag="VFZ86_08915"
     CDS             <1..1138
                     /locus_tag="VFZ86_08915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEX5642443.1"
                     /translation="GPERAVDAAVAVLGKPAVASAAPRIQPLALSTATRKSLVARKPL
                     CDELRTYAGEASGAGDVEPQDLQRVKWRTVLIFVSLGIAFYVLIPQLADVSGVWSKLK
                     AADWTWALYALGASALTYLGATLTVTGAVSSRVPFFSTLGTQLAGSFVNRIVPARVGG
                     IATNVRYLQKQGIDLAVASSSVGLQQLSGLLVHVTLSLVFLLWAGRSGAEAFDFLPSG
                     ETVLIGLTILLALSGLLFLLPAGRGVLRKRVAPILRRAGEGVGEVVRRPLKLAELLGG
                     SVFLTLSYIAALVFSVYAFGAPDVSVASIGVVFLVGSAISSAAPTPGGIGAVEAALIA
                     GLTAVGLDRDVAVPAVFLYRIATFWLPILPGWIAFVVLQRRGDL"
     gene            complement(1184..1813)
                     /locus_tag="VFZ86_08920"
     CDS             complement(1184..1813)
                     /locus_tag="VFZ86_08920"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX5642444.1"
                     /translation="MSAALLFAALTALGLLLTRVLTPSPVSRLDAEVIGWFATQRTPT
                     LTALSTVGSDLAMTLAVFVMTAVVAVVMRVRLGRWREALVLVVCVLGEWVLFRLVNGA
                     VARERPGPMLETAGTASFPSGHAGIAVAFYGGLALIILRIVTQRGLALALASLCFAVP
                     VLVALTRLYRGMHFPTDLLGSWLLAGIWIGVVVATLLPRPRAGRGPTQP"
     gene            2054..4468
                     /locus_tag="VFZ86_08925"
     CDS             2054..4468
                     /locus_tag="VFZ86_08925"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEX5642445.1"
                     /translation="MTQAHAVTSPFPAPPEIEEPAVERHVRSPADALRLTVGVIVSLL
                     GLLLAAGGSNAAVGFDRDILGVFENLPDAVERVLIATAQAVATLFPFVVLALVLYRRR
                     FRLLGMLVVAGVTAQVALAGINAGVVSLFGQPELLEAIARPGWVATAAFPDSEYIAGM
                     VAVITLAAPWMNTRWRRTAWGMVALALLFRVVSGTDLPSDLVLALGVGITSGSAWLLV
                     FGAPNRQPTGAHIAEAMQRTGIPLSRLKPASVDARSSVPYFGETADGRPVFMKVLSTD
                     QRDRNLLFRIYRFLRFKGVDDRGAFVSLQRFIEHEALVALYVNDAGTRTPRLLTVTDV
                     GDDGFLLAYERIKGDSLDSVEDDTLTDDMLRQIWREVRRLRSQRVAHRDLRLSNVFLA
                     SDGRPWMIDFGSAEMAATDLMLDLDVAELLASTAVKVGARRAVDPAVAELGKKAVAGA
                     APHLQPLALTRATSTSLAAKKPLVKELREYAAEASGAGSVEPVDLQRVKPRTLLTFAA
                     LALATYLLVPQLVGVAAYWSTLTRASWVWALWAIVASALCYVAGAFALKGAVPVRIPL
                     IPTIGAKLAGAFFNRITPATLGGMGITVRFLQKRGVDLAVATTSAGLQTITSMVATLA
                     LAVVFLAWAGASGSVTSLLPGTWTLLAIGAVLASLGLFLLLPWGRKLFRARLLPLVKR
                     AGLGMAEVARRPAKLAELLGGQLAVSLLYIAALALSVQALGGGPSLATIGVVYLFGTA
                     AASVVPTPGGIGTVEVALIGGLTAAGMPSEAAVPAVFLYRIATFWLPILPGYIAYVVL
                     QRRESL"
     gene            4544..4801
                     /locus_tag="VFZ86_08930"
     CDS             4544..4801
                     /locus_tag="VFZ86_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642446.1"
                     /translation="MRPRLLLGVLAVVLLILAIWLVPMALAAAGGSAGSPEPSGQAQA
                     PPSGTECPFLQAHPWLDPHGSADGAGGPAATSGANGQIDYY"
     gene            complement(4823..6523)
                     /locus_tag="VFZ86_08935"
     CDS             complement(4823..6523)
                     /locus_tag="VFZ86_08935"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEX5642447.1"
                     /translation="MEASLNTPAVTAISAGRRRLGALLVADGLVSQHQIEAALARQQV
                     DRRRLGDVLVREGLVSEADLVEALAAQLRLEVADLDQVYFSPETLGLLPRDFMVNRRV
                     LPLKVEDDGTLVLAMTDPLDIVAIDEVRLRTKRIVRPVICTESAFEEASATFFNTRGK
                     LRELSEEDESLVSDEAALAHDASIIEIVDGLISDAVSMKASDIHVEPHAEALHVRCRI
                     DGVLHKLREFPTEVQAGIVSRIKIMGNLDIAERRMPQDGRTSFETSSGKAVDLRLATI
                     PTLHGENVSIRILEVSPLPPTLDSLGLTGPSLERFEEAIRRPEGGILICGPTGCGKST
                     TLYTTLELVKSPQSKIYTVEDPIERKLEGVMQSEVKAAIGLDFAGLLRTLLRSDPDLI
                     MVGEVRDAETARMAAEAAMTGHLVFSTLHTRDAASAVHRLVEMGLPPYMVAAAFTCVV
                     SQRLVRRLCPHCRKPRTVRASAWEKLGLGEAPRRQMKVYDAGGCTRCFGTGYLGRAGL
                     FEVLSLDEELADMIEQGLPVRELREAARLRGVESVREDGVQKVLAGETSLSELMRVVA
                     "
BASE COUNT          892 a         2386 c         2303 g         1025 t
ORIGIN      
        1 ggggcccgaa cgcgcggtcg acgccgccgt cgccgtgctc ggcaagccgg cggtggcgag
       61 cgcggcgccg cgcatccagc cgctcgcgct cagcacggcc acgcgcaagt cgctggtggc
      121 gcgcaaaccg ctctgcgacg agctacggac gtacgccggc gaggcgagcg gcgccggcga
      181 cgtcgagccc caggacctgc agcgtgtcaa gtggcgcacg gtgctcatct tcgtctcgct
      241 cggcatagcc ttctacgtgc tcatcccgca gctcgccgac gtcagcggcg tgtggtcgaa
      301 gttgaaggcg gctgactgga cgtgggcact ctacgcactc ggcgcctcgg cgctgaccta
      361 cttgggcgct acgctcaccg tgaccggcgc cgtttcttcc cgcgtcccct tcttcagcac
      421 ccttgggacg caactggccg ggtccttcgt gaaccgcatc gtccccgccc gcgtcggcgg
      481 catcgcgacc aacgtgcgct acctccagaa gcagggcatc gacctcgcgg tggcatcgtc
      541 gagcgtcgga ctgcagcaac tctccggcct tctcgtacac gtcaccctgt ccctcgtctt
      601 cctgctgtgg gccgggcgca gcggtgccga ggccttcgac ttcctgcctt cgggcgagac
      661 cgtgctcatc ggcctcacga tcctcctggc tctcagcggg ctcctgttcc tcctgccggc
      721 gggccgcggc gtgctgcgca agcgcgtcgc gccgatcctc cgtcgcgccg gggagggcgt
      781 cggcgaggtc gtgcgccggc cgctcaagct ggcggagctc ctcggagggt cggtgttcct
      841 gaccctctcg tacatcgccg cactggtctt ctccgtgtat gccttcggcg ctcctgatgt
      901 gagtgtggcc agcatcggcg tcgtgttcct ggtcggctcc gccatcagct cagccgcgcc
      961 gactccggga ggcatcggcg cggtggaggc ggcgctcatc gccgggctca ccgcggtcgg
     1021 cctcgaccgg gacgtggcgg tgcccgccgt gttcctgtac cgcatcgcca ccttctggct
     1081 gccgatcctg ccggggtgga tcgccttcgt ggtgttgcag cggcgcggcg acctgtgacg
     1141 accgacgacc tccgacgacg gagccgctga gcctctgctc cgctcagggc tgggtcgggc
     1201 cccgaccggc tctcggccgc ggcagcaacg tcgcgacgac gacgccgatc cagatcccgg
     1261 cgaggagcca cgacccaagc aggtcggtcg ggaagtgcat cccccggtag aggcgcgtca
     1321 gggcgacaag gaccggcacc gcgaagcaca gcgacgcgag ggcgagcgcg agccccctct
     1381 gggtcacgat ccgcaggatg atcagcgcga ggccgccgta gaaggcgacc gcgatgccgg
     1441 cgtggccgga ggggaagctg gcggtgcccg ccgtttccag catcggtccg ggacgctcac
     1501 gcgcgacggc gccgttcacc agccggaaca ggacccactc ccccaggacg cagaccacca
     1561 gcaccagcgc ttcgcgccag cgccccagcc ggacgcgcat cacgacggcg accaccgcgg
     1621 tcatgacgaa caccgcgagg gtcatggcga ggtcgctgcc gacagtcgac agggcagtga
     1681 gcgtcggcgt gcgctgggtc gcgaaccagc cgatcacctc tgcgtccagg cgcgacaccg
     1741 gcgagggcgt gagcacgcga gtgaggagca ggccgagcgc cgtcagcgcc gcgaagagga
     1801 gcgccgcgga caagccgagg cgagccgccc gacggaccgc gtgtggtggg cgagacatcc
     1861 cgttctcgag catcgacggc ctctcgtggt ggcggccctt gtctcgtagt aggtctccgc
     1921 aaccgccgca tctacactcc ccacgcgttg gggaaggccc ggccgcgcaa cggtcatcaa
     1981 cggacgactg cgggaagagg actaccgggg ctcctcgcaa ctcccgacca ccgccgcaga
     2041 cggagggaac gtgatgaccc aggcacacgc ggtgacatcg ccgttcccag cgcctccgga
     2101 gatcgaggag ccggccgtcg agcgccacgt gcgctcgccg gcggacgctc tgcgcctgac
     2161 cgtcggcgtc atcgtctcgc tgctgggcct gctgcttgcc gccggtggct ccaacgcggc
     2221 ggtcggcttc gaccgcgaca tcctcggggt cttcgagaac ctgcccgacg ccgtggagcg
     2281 cgtccttatc gccaccgccc aagcggtcgc cacgctgttc cccttcgtcg tgctggccct
     2341 cgtgctgtac cggcggcggt tccgcctgct gggcatgctc gtcgtggccg gagtcaccgc
     2401 ccaggtcgcg ctggccggca tcaacgccgg cgtcgtctct ctgttcgggc agccggagct
     2461 gctcgaggcc atcgctcgcc ccggctgggt agccacggcc gcgttccccg actcggagta
     2521 catcgcgggg atggtggcgg tcatcaccct ggccgcaccc tggatgaaca cccgctggcg
     2581 gcgcacggct tggggaatgg tcgcactggc gctgctgttc cgcgtcgtct cgggcaccga
     2641 cctccccagc gacctcgtgc ttgcgctcgg cgtcggcatc acgtcgggct ccgcgtggtt
     2701 gctggtcttc ggagccccca accggcagcc cacgggagcg catatcgccg aggccatgca
     2761 gcggaccggc atcccactgt cacggctcaa gcccgcgtcg gtcgacgccc gcagctcggt
     2821 cccctacttc ggggagaccg ccgacgggcg tcccgtcttc atgaaggtgc tctcgaccga
     2881 ccagcgcgac aggaacctgc tgttccgcat ctaccgcttc cttcgcttca agggcgtcga
     2941 cgaccgcggc gcgttcgtgt ccctgcagcg cttcatcgag cacgaggcgc tggtcgcgct
     3001 ctacgtgaac gacgcgggga cgcgcacgcc ccggctgctc acggtgaccg acgtcggtga
     3061 cgacggcttc ctgctcgcct acgagcgcat caagggagac tcgctggaca gcgtggagga
     3121 cgacaccctc accgacgaca tgctgcgcca gatctggcgg gaggtgcgcc gcctgagaag
     3181 ccagcgcgtc gcgcaccggg acctgcggct ctccaacgtc ttcctcgcct ccgacggacg
     3241 cccgtggatg atcgacttcg gatccgcgga gatggcggcg acggacctga tgctcgacct
     3301 ggacgtcgcc gagctgctcg cctccacggc cgtgaaggtg ggcgccaggc gagccgtcga
     3361 cccggccgtc gcggaactgg gcaagaaagc agtcgccggc gcggcgcctc acctccagcc
     3421 tctcgcgctg acgagggcca cgagcacctc gctggccgcc aagaagccgc tcgtgaagga
     3481 actgcgcgag tacgccgcgg aggcgagcgg agccgggagc gtcgagcccg tggacctcca
     3541 gcgggtcaag ccgcgcacgc tcctcacgtt cgcggccctc gcgcttgcca cgtatctgct
     3601 ggtgccccag ctcgtcggcg tggcggcgta ctggtcgacc ctgacgcggg cgagctgggt
     3661 ctgggcgctg tgggccatcg tcgcgtcggc gctctgctac gtggcgggcg cgttcgcgct
     3721 gaagggcgcc gtccccgtcc gcatccctct catccccacc atcggcgcga agctggccgg
     3781 cgcattcttc aaccgcatca cgccggccac gctggggggg atgggcatca cggtgcgctt
     3841 tctgcagaag cgcggcgtcg acctcgccgt ggccacgacg agcgcgggcc tgcagaccat
     3901 caccagcatg gtcgcgaccc tggcgctcgc ggtcgtcttc ctcgcgtggg ccggcgccag
     3961 cggctccgtg accagcctgc tccccggcac gtggacactc ctcgccatcg gcgccgttct
     4021 cgcctcactc ggcctcttcc tcctgctccc ctgggggcgg aagctcttcc gtgcccgcct
     4081 gctgcctctg gtgaagcggg cgggtctggg gatggcggag gtcgcgcgcc ggcccgccaa
     4141 gctcgccgag ctcctcggcg gccagctcgc ggtcagcctc ctctacatcg cggcgctggc
     4201 gctgtcggtg caggcgctgg ggggcggccc gagtctggcc accatcggcg tcgtctacct
     4261 cttcgggacc gcggccgcat cggttgtccc gacgcccggc ggtatcggca cggtcgaggt
     4321 cgccctgatc ggcggcctca ccgcggccgg catgcccagc gaggcggccg tgccggccgt
     4381 cttcctgtac aggatcgcga ccttctggct accgatcctg ccgggctaca tcgcctacgt
     4441 ggtgctccag cgccgcgagt cgctgtgacc gggcggcatg ggtcaccagg gacgagggta
     4501 gaagccggaa acaagactta tcctctccgc aaaggggctt gtcatgcgac caaggcttct
     4561 gctcggcgtg ctcgccgtcg tcctgctcat cctcgcgatc tggctcgtgc cgatggcgct
     4621 cgccgcggcc ggcggctctg ccggctctcc cgagccgagc ggccaggccc aggcgccgcc
     4681 ttcaggcacg gagtgcccgt tcctgcaggc gcatccgtgg ctcgacccgc acggctccgc
     4741 cgacggcgcg ggaggcccgg ctgcgacgag cggtgcgaac ggccagatcg actactactg
     4801 aggactgccc gccggacgcg ggctacgcga ccacccgcat cagctcggag aggctcgtct
     4861 ctccggcgag gaccttctgg acgccgtcct cgcgcacgga ctcgacgccg cgcaggcgag
     4921 cggcctcgcg gagctcgcgc accggcagtc cctgctcgat catgtcggcg agctcctcgt
     4981 ccagcgagag cacctcgaag agtcccgctc gccccaggta gccggtgccg aagcagcggg
     5041 tgcaaccgcc ggcgtcgtag accttcatct ggcgccgtgg cgcctcgccc aggccgagct
     5101 tctcccaggc gctcgcgcgc acggtcctcg gcttgcggca gtgcgggcag agacggcgta
     5161 cgagccgctg cgagacgacg caggtgaagg ccgcggcgac catgtacggc ggcaggccca
     5221 tctcgaccag gcggtgcacg gccgaggcgg cgtcgcgcgt gtgcagcgtg gagaacacca
     5281 ggtgcccggt catggcggcc tcggccgcca tgcgggcggt ctccgcatcc ctgacctccc
     5341 ccaccatgat gaggtcgggg tcgctgcgca gcagggtgcg cagcaggccg gcgaagtcga
     5401 gcccgatggc ggccttgacc tcgctctgca tgactccctc cagcttccgc tcgatggggt
     5461 cctcgaccgt gtagatcttg ctctgcggtg acttgaccag ctcgagggtg gtgtacaggg
     5521 tggtggactt gccgcagccc gtgggcccgc agatcaggat gcctccctcc ggccggcgga
     5581 tggcctcctc gaagcgctcc agcgagggtc cggtgaggcc gagtgaatcc agcgtcggcg
     5641 gcaggggaga gacctcgagg atgcggatcg agacgttctc gccgtgcagg gtggggatcg
     5701 tggccaggcg caggtcgacg gccttcccgc tgctggtctc gaaggaggtg cggccgtcct
     5761 ggggcatgcg ccgctcggcg atgtccaggt tgcccatgat cttgatgcgg ctgacgatgc
     5821 cggcctggac ctccgtgggg aactcgcgca gcttgtgcag cacgccgtcg atgcggcagc
     5881 gcacgtgcag cgcctcggcg tggggttcga cgtggatgtc ggaggccttc atgctgacgg
     5941 cgtcggagat gaggccgtcc acgatctcga tgatcgaggc gtcgtgggcg agggccgcct
     6001 catcggagac gagcgactcg tcctcctcgc tcagctcccg cagcttgccc ctcgtgttga
     6061 agaaggtggc gctggcctcc tcgaaggcgc tctcggtgca gatgaccgga cgcacgatgc
     6121 gcttggtgcg cagcctgacc tcgtcgatgg cgacgatgtc gagggggtcg gtcatcgcca
     6181 gcaccagggt cccgtcgtcc tccaccttga gcggcaggac gcggcggttg accatgaagt
     6241 cgcgcggcag gaggccgagc gtctccggcg agaagtagac ctggtcgaga tcggcgacct
     6301 cgaggcgcaa ctgcgccgcc agggcttcga ccaggtccgc ctcggagacg agcccctcgc
     6361 ggacgagcac gtctcccagt cgccgccgat ccacctgctg gcgggcgaga gcggcctcga
     6421 tctggtgctg gctgaccagg ccgtctgcga cgagcagcgc gcccagcctg cggcggcccg
     6481 cggagatcgc cgtcacggcg ggggtgttca ggcttgcctc catggcgcac tcaccttcct
     6541 gccgcccggc cgagggctcg actgtggatg tccgggcgac atccaagtgc atcggcacga
     6601 gcgcgg
//
LOCUS       DASUMX010000181         6670 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_2076930, whole genome shotgun sequence.
ACCESSION   DASUMX010000181 DASUMX010000000
VERSION     DASUMX010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 6670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6670
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2076930"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="VFZ86_08940"
     CDS             <1..549
                     /locus_tag="VFZ86_08940"
                     /inference="COORDINATES: protein motif:HMM:NF013991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase"
                     /protein_id="HEX5642448.1"
                     /translation="ALTVQDAVLAYEAIRAAGPAGLGASERHDVREREVTVTLLEAMG
                     EARRRDAIAREYVTGFRLSFSLGADTILRCWRDGARFSAAVQTAFLTLLAETPDTLIA
                     RKNGVAAAADVSRRAAEVLAAGGALKEEGRAGLRALTRELRDEAHALNPGTTADLVAA
                     SLFIALTEGGLLGEVSALTDRW"
     gene            complement(533..1294)
                     /locus_tag="VFZ86_08945"
     CDS             complement(533..1294)
                     /locus_tag="VFZ86_08945"
                     /inference="COORDINATES: protein motif:HMM:NF013168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HEX5642449.1"
                     /translation="MDVIPVLDLMGGRVVQGFRGERQAYQAVRSVLAPGCEPVAIARA
                     LCRECGGPAVYVADLDAIAGSGDHLATVGELADRLGAQPWVDAGVSSEGAAERLLGAG
                     AARVIVGTETLSELGALRALGDALSAGQVVVSLDMGERGVLSRCPALSGRAPMQALEV
                     LAAEGLRDVILLALASVGTGAGPDVGTLRAARAAFPKLRLIAGGGVRDSEDLRRLAAC
                     GADGVLLATALHRGWIAAPDIRAVRSDGHPFTSGP"
     gene            1284..2327
                     /locus_tag="VFZ86_08950"
     CDS             1284..2327
                     /locus_tag="VFZ86_08950"
                     /inference="COORDINATES: protein motif:HMM:NF020032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX5642450.1"
                     /translation="MTSTPALPSAILVHEYVTGGGWQGPSIPPGLAREALAILRALLA
                     DLRAWGRFTVVTTRDRRLPTPGLDADRIVDLDHAVYPGSLLDLGRACGAALVVAPESG
                     GALEHVSALLADAGVMLLGSPARAVAVAADKWECHRRFVRAGLPTPPTVCVTPAGAEA
                     AAARLGYPVVVKPLDGAGCDGVSLAADARRLRTALRCPTLSRAPRVLVQGYVEGQAAS
                     VSLLVARGRSIALSLNGQQVRAGAPFAYDGGVASLPHPRSAEARQLARRAVALVPDLC
                     GYVGVDLVLGEGGCWLIEMNPRPTTSYVGLRRVIGLNMSAAVWDACRDGVLPDEVAPP
                     APAAFGAGWADDA"
     gene            2317..3408
                     /locus_tag="VFZ86_08955"
     CDS             2317..3408
                     /locus_tag="VFZ86_08955"
                     /inference="COORDINATES: protein motif:HMM:TIGR03123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEX5642451.1"
                     /translation="MTPDPLVTIGWDVGGVQVKAARVESRAGRPREIRTAVRALEIWR
                     RRDQLPDILRELAGELSVDATAAHALTMTAELSDAFRDKREGVLFVMDGLGAAFPRSA
                     VLAFDGDGVFVPLPEARTRPLAFAATNWIASALYAAPRIGEGILVDVGSTTTDIVPIR
                     DGRLACEGRTDLDRLIAGELVYSGVLRTNPNTLASTVPVRGRPCRLAAEQFAVMADVY
                     LILGQIDAEDYTCPTPDGRAASPSASRERLARLVCADREMLSDGEILGIARYLRERHL
                     QAIGEGLLQVLSRWEALRPVAVAPCGAGAFLASAVAARLGLEILPVAGLWGAAGATLP
                     AAAAACLLASHLESLPDPDVEASQRDTLP"
     gene            3405..4070
                     /locus_tag="VFZ86_08960"
     CDS             3405..4070
                     /locus_tag="VFZ86_08960"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate/uridylate kinase"
                     /protein_id="HEX5642452.1"
                     /translation="MIDAVVKVGGSLGRGERLREVCACLAEAGRRHRLLVVPGGGAAA
                     DVVREQDARFGLRPSTAHWMAVLAMDQYGLLLTDLTPGAEAVRSLDRARTRLAEEGGV
                     VVLLPSEPLRRADALPHSWSVTSDAIAAWVTQAADGRLLVLLKDHYGMAKLAPAAASE
                     TPRLMDVAAVAASGSVDGHLAGLFSAAAFDLWMIDGEQPRRLARLLDDGSTEGVKLVR
                     QAP"
     gene            complement(4045..4755)
                     /locus_tag="VFZ86_08965"
     CDS             complement(4045..4755)
                     /locus_tag="VFZ86_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010875780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(5-formylfuran-3-yl)methyl phosphate synthase"
                     /protein_id="HEX5642453.1"
                     /translation="MDLLISVVGEGEVHPAVAGGADIVDVKNPREGSLGANFPHVIRR
                     VRELTPAAVPVSVAIGDVPNLPGMAALAAAGAAGCGVQFVKVGLLGPRDHDEAYAVLA
                     AVCRAARDEAPGVRVMATAYADAHTTGSLPPAELPAVAAEAGADGCMIDTAAKGAGTL
                     FTALAAAELERFVSRCAEAGLLCALAGSLSAADVPRLRELAPDIVGFRGAACGDGRRE
                     GTLDRDAVQRLKELVAQA"
     gene            4834..5913
                     /locus_tag="VFZ86_08970"
     CDS             4834..5913
                     /locus_tag="VFZ86_08970"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEX5642454.1"
                     /translation="MPASERRRGSALEGAAVETRDGLLFTVKGVLHPPDRIVAYLRYV
                     PDPQGERERGRDRYRRVYSSADREAALRARDISWRLDDPALGVPVDAVPWTEVARVHD
                     PRARLRELQARGPADPLERDALALAALLCEASGAPSGALGLTGSLLLGLHTPASDIDL
                     VVYGDRECRRVHAALSRLLDDPSSAVARPQGKELAAIHAVHRADTPLSADDFARLQAR
                     KVNEGRFAGRPFFIRFVKLPADVGERWGDPRFAPAGEALVEARVTSDRDAIFTPCRYA
                     LDEVRSLEGASVDGLGEVISYRGRFAEQARAGHRVRAYGALEHQIWSDGREALRLVVG
                     SRPGDYLLALEEDSDPPRPAGREAP"
     gene            5910..>6670
                     /locus_tag="VFZ86_08975"
     CDS             5910..>6670
                     /locus_tag="VFZ86_08975"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HEX5642455.1"
                     /translation="MNAAGTSPRSVEVGVLIAGGGSRRAGLDKRFLVLEGRTLLVRNL
                     AFLRSLFPEVAVVIGRTQSLDLGDEAGVHVLRDAWPGSSPLVGIATALQHFGRPVFVL
                     AADIAFPQREAAEAVIAASRSRDVAMPAGANDFRQPLFAVYGPRCLPPMARLLESGRH
                     RIVDIFPSVSVAEVSGFDEALFRNVNTLADFESARRESARRSPTGARPALVAIVGKSD
                     SGKTTLIEKLVPELVKLGLRVGTVKHDAHSFEIDHP"
BASE COUNT          834 a         2504 c         2453 g          879 t
ORIGIN      
        1 gccctgacgg tccaggatgc cgtcctggcc tacgaggcca tccgcgccgc cgggcccgcg
       61 gggctgggcg cgagcgagcg ccacgacgtc cgcgagcgcg aggtcaccgt gacgctgctc
      121 gaggcgatgg gcgaggcacg ccggcgcgac gccatcgccc gcgagtacgt caccggcttc
      181 cgcctctcct tctcgctcgg cgcggacacg atcctccgct gctggcgcga cggcgcccgt
      241 ttctccgcag cggtccagac ggccttcctc accctcctgg cggagacccc ggacacgctc
      301 atcgcgcgca agaacggcgt cgccgctgcg gcggacgtgt cacgacgcgc ggccgaggtt
      361 ctggccgccg gtggggccct gaaggaagag gggcgcgccg ggcttcgggc gctgacgcgc
      421 gagctccggg acgaggctca cgcgctcaac cccggcacga ccgcggacct cgtcgcggcg
      481 tcgctgttca tcgccctgac ggagggcggc ctgctcggtg aggtgtccgc gctcacggac
      541 cgctggtgaa cgggtggccg tccgagcgca cagcgcggat gtccggcgcg gcgatccagc
      601 cgcgatgaag cgcggtggcg aggagcaccc cgtcggcgcc gcacgcggcc agccggcgca
      661 ggtcctcgga gtcgcgcacg ccgccgcccg cgatcaggcg cagcttcggg aacgccgccc
      721 gcgcggcccg cagcgtgccc acatctggcc cggcgccggt acccacgctg gccagcgcga
      781 gcaggatgac gtcgcgcagg ccttcggcgg cgaggacctc cagcgcctgc atgggcgccc
      841 gtccgctcag cgcggggcat ctgctgagga cgccacgctc gcccatgtcg aggctgacga
      901 ccacctgccc ggcggacagg gcatcaccga gcgccctcag cgcaccgagc tcggagaggg
      961 tctcggtgcc gacgatgacg cgtgcggctc ccgcgccgag gagccgctcc gcggcgccct
     1021 cggagctcac gcccgcgtcc acccacggct gggccccaag gcggtcggcc agctcaccga
     1081 cggtcgcgag gtggtcgccg ctgccggcga tcgcgtcgag gtcggcgacg tacaccgccg
     1141 gcccgccgca ctcgcggcac agcgctcgcg cgatcgccac gggctcgcac ccgggggcga
     1201 gcacgctgcg cacggcctgg taggcctgcc gctccccacg gaatccctgc accacccgac
     1261 cgcccatgag gtctaggacc gggatgacgt ccacgccggc tctcccttca gcgatcctcg
     1321 tgcacgagta cgtcaccggc ggcggctggc agggaccgag catccctccc ggactcgcgc
     1381 gcgaggcgct ggccatcctc cgagccttgc ttgcagacct gcgggcctgg ggccggttca
     1441 ccgtcgtgac gacgcgcgac cggcgtctgc cgacgccggg gctggacgcg gatcgcatcg
     1501 tcgatctcga ccatgcagtc tatcccggat cgctgctcga cctcggccgg gcgtgcggag
     1561 cggcgctggt cgtggccccg gagagcggag gggcactgga acacgtgagc gcgctgctgg
     1621 ccgacgccgg agtcatgctc ctgggcagcc cggcgcgcgc ggtcgccgtc gccgcggaca
     1681 agtgggagtg ccaccgtcgc ttcgtgcgcg ccgggctgcc gacgccgccc accgtctgtg
     1741 tcacgccggc cggcgccgag gcggccgcgg cgagactggg ttacccggtc gtggtgaagc
     1801 cgctcgacgg cgccggatgc gacggcgtca gcctggccgc ggatgctcgg cggctgagaa
     1861 cggccctgcg ctgcccgacc ctgagccggg ctccccgggt gctcgtgcag ggctacgtcg
     1921 agggccaggc ggccagcgtc tccctcctgg tcgcccgggg gcgctcgatc gccctgagcc
     1981 tgaacgggca gcaggtgcga gcaggcgcgc cgttcgccta cgatggcggg gtcgcgtccc
     2041 tgccgcaccc acggagcgcg gaggcgcggc agctcgcgcg aagggcggtc gccctggtgc
     2101 ccgatctgtg cgggtacgtg ggcgtcgatc tggtgctggg tgagggcgga tgctggctga
     2161 tcgagatgaa tccgcggccg acgacatcgt acgtcgggct gcgccgcgtg atcggtctca
     2221 acatgagcgc cgcggtctgg gacgcctgcc gcgacggcgt cctgccggac gaggtcgcgc
     2281 cgccggcgcc ggcggcgttc ggagcagggt gggccgatga cgcctgaccc cctggtgacc
     2341 atcggctggg acgtgggcgg cgtgcaggtc aaggccgccc gggtcgagtc acgggccggc
     2401 cgtcctcgcg agatccggac ggccgtgcgt gctctcgaga tctggcggcg aagagaccag
     2461 ctcccggaca tcctcaggga gctggcgggc gagctctccg tggacgccac cgccgcgcac
     2521 gccctcacca tgacggcgga gctctcggac gcgttccggg acaagcgcga aggcgtgctg
     2581 ttcgtgatgg acggcctcgg cgcagccttt ccgcgcagcg ccgtcctggc cttcgacggc
     2641 gacggcgtgt tcgtccccct gcccgaggcg cggacacgac cgctcgcgtt cgcagccacg
     2701 aactggatcg cctcggccct gtacgccgcc ccgcgcatcg gcgagggcat cctcgtggac
     2761 gtggggagca cgaccaccga catcgtcccc atccgcgacg gacggctcgc gtgtgaagga
     2821 cgcacggatc tcgaccggct catcgccgga gagctggtgt acagcggcgt cctgcgcacc
     2881 aatcccaaca ccctggcgag tacggtgccg gtccgcggcc gcccctgccg gctcgcggcc
     2941 gagcagttcg cggtgatggc cgacgtctac ctgatcctgg ggcagatcga cgcggaggac
     3001 tacacctgcc ccacgccgga cggacgggcc gcctcgccct ccgcgtcgcg cgagcgcctg
     3061 gcacgcctcg tgtgcgccga ccgcgagatg ctgagcgacg gcgagatcct cgggatcgcg
     3121 cgctacctgc gcgagcgaca cctgcaggcg atcggcgagg gtctcctcca ggtcctgtcg
     3181 cggtgggagg cgctccgccc ggtcgccgtg gccccgtgtg gcgccggcgc cttcctggcg
     3241 tccgcggtcg ccgcgcggct cggcctcgag atcctcccgg tggccggcct gtggggagcc
     3301 gccggcgcga ccctgccggc cgccgccgcc gcgtgccttc tggcgagcca cctcgagagc
     3361 ctgcccgatc ccgacgtcga ggcgagtcag cgagacacgt tgccgtgatc gacgcggtcg
     3421 tcaaagtcgg tggcagcctg ggccgcggcg agcgcctgcg cgaggtgtgc gcctgcctgg
     3481 ccgaggcggg gcgtcgccat cgtctcctgg tcgtcccggg cggcggggcg gcggccgatg
     3541 tcgtgagaga gcaggacgcg cgtttcgggc tgcggccgtc gacagcgcac tggatggccg
     3601 ttctcgccat ggaccagtac gggctgctcc tgaccgacct cacgcccggc gcggaggcgg
     3661 tccgctcact cgaccgggca cgcacgcgcc tcgcggagga gggcggcgtc gtcgtcctcc
     3721 tcccgtcgga gccgctgcgg cgcgcggacg ccctgccgca cagctggtcg gtgacctccg
     3781 acgccatcgc cgcgtgggtc acgcaggcgg cggatgggcg cctcctggtg ctgctcaagg
     3841 accactacgg catggcgaag ctcgcgccgg ccgcggcgag cgagaccccg cgcctcatgg
     3901 acgtcgccgc ggtggccgcc tccggcagcg tcgacggtca cctggcagga ctcttcagcg
     3961 cggccgcgtt cgatctgtgg atgatcgacg gcgagcagcc gcggcgcctc gcgcgcctgc
     4021 tcgacgacgg gagcacggag ggggtcaagc ttgtgcgaca agctccttga ggcgctggac
     4081 ggcgtcgcga tccagcgtgc cctcccggcg gccgtcgcca caggcggcgc cgcgaaagcc
     4141 gacgatgtcg ggagcgagct cacgcagccg gggaacgtcc gcagcgctca gcgagccggc
     4201 cagcgcgcag agcaggccgg cctctgcgca gcgggacacg aagcgctcga gctcggccgc
     4261 ggccagcgcg gtgaacagcg tccccgcccc cttggcggcg gtgtcgatca tgcagccgtc
     4321 cgcgccggct tccgcggcga cggcgggcag ctccgccggc ggcagagaac cggtcgtgtg
     4381 agcatcggcg taggcggtcg ccatgacccg cacgccgggg gcttcgtcgc gcgccgcgcg
     4441 gcagaccgcc gccagcacgg cgtacgcctc gtcgtggtcg cgcggtccga gcagcccgac
     4501 cttgacgaac tgcacgccgc agcccgcggc tcccgccgca gccagggcgg ccatgccggg
     4561 caggttgggg acgtcgccga tggcgacgct cacgggaacg gcagccggcg tgagctcgcg
     4621 cacgcgccgg atcacgtggg ggaagttcgc gccgagcgag ccctctcgcg ggttcttgac
     4681 gtcgacgatg tccgcgccgc cggccaccgc gggatggact tcaccctcgc ccaccacgct
     4741 gatgagtagg tccatgacgg tcggccctcg actctcctcg acgcccgcca gcgtacatgc
     4801 gctgccgacg ggcacgcaag cggggtgctg acgatgccgg cgagcgagcg gcggcgcgga
     4861 tcggcgctcg agggcgccgc cgtcgagacc cgcgacggct tgctgttcac ggtcaaggga
     4921 gtcctgcacc cgcccgaccg catcgtcgcc tatctgcgct acgtgcccga tccgcagggc
     4981 gagcgagagc gcggacggga ccgctaccgc agggtctaca gcagcgccga ccgcgaggcg
     5041 gcgctgcgcg cgcgggacat ctcctggcgg ctcgacgacc cggcgctcgg cgtgcccgtg
     5101 gacgccgtgc cgtggaccga agtggcgcgc gtccacgacc cgcgcgcgcg cctgcgcgag
     5161 ctccaggcga gggggccggc ggatccgctc gagagagacg cgctcgcgct cgcggcgctg
     5221 ctgtgcgagg cctccggcgc accttccggc gcgctcggac tgaccgggtc gctgctgctc
     5281 ggcctccaca cgccggcgtc ggacatcgac ctcgtggtgt acggcgaccg ggagtgtcgc
     5341 cgggtgcacg cggcgctctc gcgcctgctg gacgatcctt cctcggcggt ggcgcgcccc
     5401 cagggcaagg aactcgcggc catccatgcc gtgcaccgcg cggacacgcc actgtccgcc
     5461 gacgatttcg cccgcctgca ggcgcgcaag gtgaacgaag gccggttcgc cggccggcct
     5521 ttcttcatcc gcttcgtcaa gctgccggcc gacgttggcg agcgctgggg ggatccgcgc
     5581 ttcgcgccgg cgggagaggc cctcgtggag gcccgggtga ccagcgaccg cgacgcgatc
     5641 ttcaccccct gccgctacgc gctcgacgag gtgaggtccc tcgaaggcgc ctccgtcgac
     5701 ggcctgggcg aggtgatctc gtaccgggga cgcttcgccg agcaggcgcg cgccggtcac
     5761 cgggtccgcg cgtacggcgc gctcgagcac cagatctgga gcgacggtcg tgaggcgctg
     5821 cgcctcgtcg tgggcagccg cccgggcgac tacctgctgg cgctcgagga ggactcagac
     5881 ccgccgcgac cggcgggccg ggaggcgccg tgaacgcggc gggcacctcc ccccgatccg
     5941 tcgaggtcgg cgtgctcatc gccggcggcg gaagccggcg ggccggcctg gacaagcgct
     6001 tcctcgtcct cgagggacgt acgctcctcg tccgcaacct cgcctttctc cgcagcctct
     6061 ttcccgaggt ggccgtggtc atcggccgca cgcagagcct cgacctcggt gacgaggccg
     6121 gcgtccacgt cctcagagac gcgtggccgg gctcctcgcc gctcgtcggc atcgccacgg
     6181 cgctgcagca cttcgggcgg ccggtgttcg tgctcgcggc ggacatcgcc ttccctcagc
     6241 gggaggccgc ggaagccgtc atcgcggcct cccggagccg cgacgtcgcg atgcccgcgg
     6301 gcgcgaacga cttccgccag ccgctcttcg ccgtctacgg gccgcgctgc ctccccccca
     6361 tggcgaggct cctcgaatcg ggtcgccacc gcatcgtcga catcttcccg agcgtctccg
     6421 tggccgaggt gagcggcttc gacgaggctc tctttcgcaa cgtcaacacg ctcgccgact
     6481 tcgagagcgc ccggcgtgag agcgcccggc gctcgcccac gggcgcacgg cccgccctcg
     6541 tcgccatcgt cggcaagagc gactccggca agaccaccct gatcgagaag ctggtgcccg
     6601 agctggtgaa gctcggcctg cgcgtcggca ccgtcaagca cgacgcgcac tcgttcgaga
     6661 tcgaccaccc
//
LOCUS       DASUMX010000182        23432 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_208133, whole genome shotgun sequence.
ACCESSION   DASUMX010000182 DASUMX010000000
VERSION     DASUMX010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 23432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23432
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208133"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..1509
                     /gene="mtbB"
                     /locus_tag="VFZ86_08980"
                     /pseudo
     CDS             124..1509
                     /gene="mtbB"
                     /locus_tag="VFZ86_08980"
                     /inference="COORDINATES: protein motif:HMM:NF021053.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="[dimethylamine--corrinoid protein]
                     Co-methyltransferase"
     gene            1565..2248
                     /locus_tag="VFZ86_08985"
     CDS             1565..2248
                     /locus_tag="VFZ86_08985"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEX5642456.1"
                     /translation="MRREDVVDQDRRDLVMFDFDGVIVDSLDVFGAAFLDACAATGVE
                     GFTRTDDLLAVMESNFFTGMRARGIDDERVAEVLRRVGDALIRARHWLKPFPLVPDVL
                     EDLADTRTVVVVTSSPGEVVAGWLRTHQVHGVTEVAGAESAQSKVEKIHALLARFPGQ
                     EVYWYVGDTAGDIREAREAGVTPLGVAWGWHEPEMLLEAGAERIVASPAELLAVVAPE
                     QARDFFGAG"
     gene            complement(2274..2759)
                     /locus_tag="VFZ86_08990"
     CDS             complement(2274..2759)
                     /locus_tag="VFZ86_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642457.1"
                     /translation="MTGAPRIVGRRFPSVAGTALSGELVHVPEDFAGAPVLLLCAYRR
                     GTQADVDRWAAFAERRLPGLAVRELPIISSRLWRPLQGWIDGGMRGGVPPSLWGSVVT
                     LYEEGAAARAFIGDGGGQRTQVVLLDGAGVVAFHDAGGFGEEAAEELVAAAAALGRRE
                     G"
     gene            complement(2822..3994)
                     /locus_tag="VFZ86_08995"
     CDS             complement(2822..3994)
                     /locus_tag="VFZ86_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642458.1"
                     /translation="MELSDRPLEAELAEIAEHAEPGRTARLAVRHWGWDGRGGATLET
                     TGRELGGITRERVRQLCERLAGRLAEPRVAAPALERALVLAAHAAPTTAHDLARRLAD
                     ARIASQPFDPAGVLTAAAVLGRDATFTLESVKGVRVALPSPPDPENDTVHVIAAIADT
                     ARGLVRRAGAARVGEVTGRVAADLAVWVDDELVTAVVSEPADFAWLERRTGWFSLPSV
                     ARNAVVSRLAKVLSVGGAVSLADLHAGIRRDERMREFVMPEYILAELCARVPGVTVSG
                     SLVIADQPQAPEEVLETTELTLLRALMRAGGEADRHELERACVAAGMKLSSFNNRIAY
                     SPIISDLGQGRYGLRGLGGGGDGRASWRAAGDAGAAADDEVPRPGDLAAFRPARRR"
     gene            4189..5001
                     /gene="ppk2"
                     /locus_tag="VFZ86_09000"
     CDS             4189..5001
                     /gene="ppk2"
                     /locus_tag="VFZ86_09000"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008675934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HEX5642459.1"
                     /translation="MSTPPAEKPGHHKKLDREQYEAELEKLQVELVKLQEWIKHAGLR
                     VAVIFEGRDAAGKGGTIKRVTEHLNPRVVSIAALATPTERERTEWYFQRYVEHLPSAG
                     EMVLFDRSWYNRPGVERVMGFCTEDEVWEFFRTCPEFERMLIRSGIILLKYWFSVSDE
                     EQEKRFQARLKDPLKVWKLSPMDLESRMRWVEYSQAKDEMFAYTDTKDSPWWVVNADD
                     KRRARLNMIRHLLSQIPYEDVTHKDLKLAERPPDKGYVRPPIDSQTFVPEIY"
     gene            complement(5035..7395)
                     /locus_tag="VFZ86_09005"
     CDS             complement(5035..7395)
                     /locus_tag="VFZ86_09005"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HEX5642460.1"
                     /translation="MAALLVAVWVAPRASGAAADWIRTPSVRLHSVSPSDDGRLVVAG
                     PSARGAGAPVTLDAGMEFTMAGVTCDVPATGAVTLRLRTSLDGSAWGPWLEAPLELGG
                     DGAVATAFTDALWTGPARFAQVAAATGARGGPAALTGVRLVAIDPTESGGIAARVGGA
                     ARRFAATVAGVSLDSPASAAAAAPVIVTRSEWGADESLRNAAPSYAPVKMAFVHHTAS
                     GNVYSRDDAPALVRGIYAYHTKSLHWNDIAYNFLVDRFGNVYEGRYGGVSRGVAGAHV
                     YGFNTGSTGVSVMGTFIDEAPPAEAVAALQRLLAWKLAVHGLDPQGAARLTCGAGDKY
                     AAGATVAFPVIAGHRQANYTECPGAALYGLLPSVRANVAKRVGSALVATLRASTPLIS
                     PNGDGVLDTTVLDVGVSAPADWRVAVRAAGGQAVASWSGQGTSAAITWNGASGGARVP
                     DGVYTAELTATPEGGEPAVTSVQITVDTVAPRLSGASSTGSFSPNGDGQAESAAVAYT
                     PAEACAVRVGILDAQGDALRWLHGWRSRETRSYSVGWDGRITSGGGLVAAPDGQYRFT
                     VERRDAAGNIARQGVRISVDRTIGFPAAVPVTLSPDGNGVRDATALTFKLTRKATVTV
                     RVLLDAVVVRTLSLGALAAGAHSATWDGRAESGEDLASSRPTFTVTAVSTLGESRVTK
                     GLVVDLYRPRLYAAGGRTTSVGTAAKLGFKVTDPFSAKADVRYAITDAKGRRVASGHP
                     GWRPTGTAQSISWRPRARGVYTVTYRAVDLGGNREAATAHTVVTVR"
     gene            complement(7651..8052)
                     /locus_tag="VFZ86_09010"
     CDS             complement(7651..8052)
                     /locus_tag="VFZ86_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642461.1"
                     /translation="MPSPDALSGPSAEGRRLSSTGGGSLLPLAGCVPVPGPRRRPVTA
                     VAVDDELVWVTAVPALELGTARAPSPAAFVHLCVQLAAALAAAGGPALEAALSDADLV
                     DAAARAVAGYLDDLPDLPLVGEPFGGEEGTD"
     gene            8207..8422
                     /locus_tag="VFZ86_09015"
     CDS             8207..8422
                     /locus_tag="VFZ86_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HEX5642462.1"
                     /translation="MSDSVYRVMEVIGSSSESWEQAARNAVEKAGIHREDLRIAEAVE
                     FDMKIEGKQIVSYRTKVKISYKYHEIV"
     gene            complement(8435..9850)
                     /locus_tag="VFZ86_09020"
     CDS             complement(8435..9850)
                     /locus_tag="VFZ86_09020"
                     /inference="COORDINATES: protein motif:HMM:NF017885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEX5642463.1"
                     /translation="MRDEHQTSIGALIASLREQQGWSQRALAKWVGIDQSAVSRIEAG
                     RRRLSADELQRFADALHVSADELLGAAPAIHSLVTDDAAASAAGPPSSARAHLMHASQ
                     AAPEHAALERVVRPAPRHAFRSYVSGPDAPFGRPGLAGLPGEVAGVARDWFALHELAK
                     DPEPAQRWSTGHSAEGKPGRVAHSAASPRGGAPDADVLYDRVARFWRSELHVDPDDGP
                     LPDLVPLLEDGYGAQVIVARVAGATAPVSGADTPPAAETPVAAAFADAGVPFVFVNAA
                     RPVVLQRFALAHAFAHLVLGHGDVVDHHIEWSRNAPPEAAANDFAEELLAPVRAVQRW
                     YDRRAASRVTGVDDLLHLGNAFGISAWAALYRSRAAGRLQGKQFHVLRSELRRHEWEL
                     LPRQAYLGGLRDTLTHLTPDEALPAGEYGRPAVLRVPAAMRAWALSAVRGGRLSLEGA
                     AAMLHLEPGGLAEQLARLGLE"
     gene            10079..11128
                     /locus_tag="VFZ86_09025"
     CDS             10079..11128
                     /locus_tag="VFZ86_09025"
                     /inference="COORDINATES: protein motif:HMM:NF042637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6569 family protein"
                     /protein_id="HEX5642464.1"
                     /translation="MTKNNEVPGGAALVEALRLGERQAHGPLAVFPLRLGPGDGPGYI
                     TLREAIGRQQATVTEVSDGGSVPELRVVNKGDERVLVLDGEELRGAKQNRVLNTTILI
                     GGHSSLVVPVSCTEQGRWSYASREFEESEIVADRQVRYAMKESINASLVAGAGHRSDQ
                     GRVWHEVGELHGRHATTSPTGAMRDAYQMRKGDLDAVLAAFPLQDGQQGMLVLHGARV
                     VGLDLVSRAPQYAELHDKLLRSYAFEALVRDGEPGDRAVADAFLERIADLPGSRYKSP
                     GLGWDVRYEGGGVLGSALTYRGHAVHAAFFDVGGVRGASDDGGSGGDPRGASRPRPDW
                     RIADARERARRRQAH"
     gene            11140..11988
                     /locus_tag="VFZ86_09030"
     CDS             11140..11988
                     /locus_tag="VFZ86_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642465.1"
                     /translation="MTEDTRQRAYKAAAHITREQLRRDDPERAVILEQVESSDVIVVA
                     GIYDHVESVLGALDVPHLVVQPHELDQLALRPEQLLIVNCPGQIAPAAVRRVRAFVEA
                     GGSLFTTDWALKHVIEPAFPGVLAFDTAPTRDDVVRIEVLDGDNIYLQGVLDGQDDPQ
                     WWLEASSYPISVVDEARVEVLITSRELGERYGERPVAVWFRWGEGDVFHMISHYYLQR
                     TEERSARHAAPAGAYFAEKGVPMGQEMCQDVADLSVADVESAKPSTTFMSNVIIEKKR
                     RQAREG"
     gene            11988..12551
                     /locus_tag="VFZ86_09035"
     CDS             11988..12551
                     /locus_tag="VFZ86_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642466.1"
                     /translation="MRVTTDLRPGIAVEFEQVQGVPMAWVRLPDLADVPDDDLRRACR
                     HAVNEAFVAQGAERGSVLARALKPLDEELRRRGLEPDGWPVVGPDPACAPSAAWGEGP
                     GREDDVPPPRQGLPSLRGLDEAQLRQAHLCAMRRVFHATGPACIGMRATLLEPVEAEM
                     RRRGLAARYWSALDRSRRAAAFWSALN"
     gene            12710..14302
                     /locus_tag="VFZ86_09040"
     CDS             12710..14302
                     /locus_tag="VFZ86_09040"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5642467.1"
                     /translation="MSAWSPLRYRVYRYLWIALLVSNIGTWMQAVGAAWLMVELDASP
                     AVVALVQTATYLPVVVLGVLAGAAADLIERRRLLLVTQMIMLCAAGALAVLSGLGLVT
                     PFWLLVLTFALGVGATLNAPAWQAIQPEIVPPQELKQAVTLGGANINLGRAIGPAVGG
                     LLIVAAGPWLVFALNAASFAFVLAVLWRWKRPVEEQVGPPERFAGAVRAGIRYAMFSH
                     ILHGVLVRSFAFGVASSGFMSLLPVYASEELGWGSGGLGILFAAMGLGAVLTTAVVPS
                     LRERLSADGVFAVGSALVAAGLVTLALLRAPTPAVVASFVIGTGWLCCLSTLNVASQE
                     VLPGWVRARGLALYLTAISAGIALGSALWGKLANGTGAPAAYAWGALAIVVTVGLAWR
                     WRFDRIADVDLRPAPMAAPEARLVAGQDADSPALVVVAYEVREDAEDDFLRALRLVGR
                     VRRRTGATDWSVYRDADKPRRFIETFVLPSWQEHLRQHQRRTVTDLELQEDVRQYLRP
                     GAEPTAKHFVAPPEPSMRFWSR"
     gene            complement(14310..17081)
                     /locus_tag="VFZ86_09045"
     CDS             complement(14310..17081)
                     /locus_tag="VFZ86_09045"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEX5642468.1"
                     /translation="MYLDADRPAPPLATRDAAAAACADGDRSGPRLLFFDVPSRRDLA
                     QRAAAAAPGARFASRRAWTEDPGEPRRPLLGGEVPVGLWYWKGSRDEFEAQATEILLR
                     LVHPLVARGLPFCLGLPFAEDVLEPRRLAVGDAARLDLEDIGRLLRAATAGRGDPPAR
                     AAAGSSEPAVVGLPAAGLPTAPLPTRDGRPSSGPDAVQLDAAQRAAVEHARGPARVLA
                     PAGSGKTKTLISRVVELVDRGADPSGILMLAFNRKAAEQLEERLAALGIASTRRLGSP
                     LEEASPSRRPRRRGGPPGHPGRPSGVHCATFNAFGYRYQREVLRARFSLDHDGRSLRA
                     LMARAMESAGVSLRELKPRRGSDPVGAFMQGLTRVRAALEPPAGVEVRIESLGDTPLL
                     DLPFAPIHAQYARAQAATGLQSFDDQIYFAVADMLAEPSHRAFIQARFDHVLVDEFQD
                     LNGAQLALVDVLSRPHRRLFVVGDDDQLIYGWRQADPRGILEFHRRMPPRPWSATYTL
                     CTNYRCSRAVVETGARLVARNLVREAKDIRPREGAQDGAVRFFGAASWPERAATICAF
                     LHAEKQRLGCDWRDLAVLCRYRSQQLLVALALDAGDVPRTPALGCTLFTHPAATLLRA
                     YLDLVRAPDELPGPALAGLLNRPNRYLSAAVVEAIGAASRPWARLRAIGAQEPPAGPR
                     PLSALVELVDRLGPELATAEVLTSEDLVWTVVEAFTLDDLWAAEADAAAAGDGAGSDG
                     AGPLEVFDSALLLAETYPDPGEYLRTWDRLRADEEAHAGLADDTLAREESEEDRVVIG
                     TIHAAKGREYDSIVIPDYDCDVSRWSASEVEEERRVVYVGVTRARDAALFTVDSTSGF
                     VHQFLRELVEAPDPGEHEALSAWLAEEPGAELRELIAGRLAEIEVLFPELVPQEEDAA
                     PGAAGPSPP"
     gene            complement(17167..17742)
                     /locus_tag="VFZ86_09050"
     CDS             complement(17167..17742)
                     /locus_tag="VFZ86_09050"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX5642469.1"
                     /translation="MTTPRIERVAPWVARAPDVPAWWFRRASRLHGENHTRRVHVHAQ
                     RLLERLRWDEADARLVLRAALWHDIGRETDGVEPPHGARGAARADELGLTAGLAPDDA
                     ALVRFAITRHSLPDGGAAAQAAELAVAEDPARCPARPERALRILWLLKDADALDRVRL
                     GFGERADPRQLRHPEAIDQIGFADRLYAALK"
     gene            complement(17797..17961)
                     /locus_tag="VFZ86_09055"
     CDS             complement(17797..17961)
                     /locus_tag="VFZ86_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="C2H2-type zinc finger protein"
                     /protein_id="HEX5642470.1"
                     /translation="MSLEMHPCPHCPAEFDSLKSLEHHIKASHHEDLPDEKFRCSTCD
                     DAFMKQVEWL"
     gene            18115..18660
                     /locus_tag="VFZ86_09060"
     CDS             18115..18660
                     /locus_tag="VFZ86_09060"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020475308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HEX5642471.1"
                     /translation="MDAHPPDMTLADRMLVVEGDITRLDVDAIVNAANSSLLGGGGVD
                     GAIHRAAGPGLLAECRGLGGCATGEARITRGYDLPARHVIHTVGPVYRQHDPAEAERL
                     LRSCYTESLRLACEAGLETVAFPGISTGVYGYPKAEACDAAVRAVAAWLAAHALPRAV
                     TFCCFGAEDAALHRARLGLTS"
     gene            18698..19855
                     /locus_tag="VFZ86_09065"
     CDS             18698..19855
                     /locus_tag="VFZ86_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642472.1"
                     /translation="MASPRWSDFGSIWKTIREVDVLAIRREAEHDLTVACAGDREALE
                     YLRGLLLEGPDRYPSPFTALGLVPLDQVHARERLIREADLLLVALRADVSLSPGEVAG
                     LERLESLSPRRRLLVLVGEPAAPSRSWLRRLDEGKTLVVRPEAAGSPDRLAAAVLEAL
                     PPQRQLAAARRLPGLRLQYARRLTAEVSLSNGAFALASGVPSLVPFLGVPITAADTVV
                     LTKNQALMVYRLALAFGAPPEFQRRMMEITPVIGAAVVWRQVAGGLVGLVPGYGILPK
                     TAVAFAGTYITGRAAELWYATGLVSKDDMRRISAEATAKARSMAGEQARRAREARDAA
                     GRARRATTRVRENARRLTRRRGPRTTSDAPPAGQPAPGAFLDAPPASPEKE"
     gene            complement(19952..20332)
                     /locus_tag="VFZ86_09070"
     CDS             complement(19952..20332)
                     /locus_tag="VFZ86_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010239681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diol dehydratase small subunit"
                     /protein_id="HEX5642473.1"
                     /translation="MTPGVARTPRALSGRPVEEITLEAAVRGDLSPADLRVHPDVLRH
                     QAAVAEEHGNPQLGENLRRAAELTALPDAEVLAVYEALRPGRSTFAGLEAIAARLETA
                     DAPLCAALVREAAEVCARRGLLAG"
     gene            complement(20329..22653)
                     /locus_tag="VFZ86_09075"
     CDS             complement(20329..22653)
                     /locus_tag="VFZ86_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011731202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol/glycerol family dehydratase large
                     subunit"
                     /protein_id="HEX5642474.1"
                     /translation="MTNGDERIPAAGDVPPRLGRLDALDERPVNLDGFAQRDDEAGLV
                     AFASANDPEPSVALDADGRVVELDGRAAADYDLIDAFIAARGLDPVVAMEANAVDDIA
                     FARLIVDPGTPRAEVVRLAAGATPEKLARVVALLQPVELQMAVAKMRVRRTPSNQAHV
                     TNRLDDPLLLAADAATAVAYGFREVETTVPVNADAPSNALAVLIGSQVGACGALTQCS
                     IEEALELELGLRGLTTYAETVSLYGTEKAFIDGDDTPWSKAFLCSAYASRGLKMRVTS
                     GGGAEALMGAAEGRSMFSLESRCVALARAMGAQGVQNGGIDGASVVGCVPDGMRELLA
                     ENLLVMLRDLESCSGNDALVSESDMRRTAHTVPLFLAGSDFLFSGFGSIPRYDNMFGP
                     SNFNAEDLDDYLVLQRDWGVDGGLRPVTEQRMREVRERAAKACLAVYRELGLIAPGDE
                     PTAGFTDDHVGEVVVAAGSKDVTPPDPQAILAASDAIMERQIGILDVVRALFATGFEE
                     EARRVLAMGRARVAGDYLQTAAIFDEQMDCLSKVTDPNDYRGPGTGYEVPAARREEIA
                     AIRQQRSRADLLARHAAGAPPATAGPQPFRLVERGPAQVGADPREVCIGVSPAFATKL
                     WLTMSGLPVGEALAEVMAGIAAEGCTPRVVRVSSTVDLGVIGLTAAELAGSGVGVGLQ
                     AKGTALIHRKDQAPLACLELLSIAPLLTAAMYRELGANAGRHAKGLRPAPVRNPYTDQ
                     AIEARYHAEVVSLIAVERGESVPGAPPVDLETRR"
     gene            22915..23160
                     /locus_tag="VFZ86_09080"
     CDS             22915..23160
                     /locus_tag="VFZ86_09080"
                     /inference="COORDINATES: protein motif:HMM:NF015947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEX5642475.1"
                     /translation="MKAKVQRWGNSLAVRIPMSLARELGLHDQSAVALRQDQGRIVIE
                     LLRSAPTLEELLAEVTQQNKHTDLDWRPAGGLETGER"
     gene            23157..>23432
                     /locus_tag="VFZ86_09085"
     CDS             23157..>23432
                     /locus_tag="VFZ86_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012031924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEX5642476.1"
                     /translation="MKYWRPWTPACGDIVWVRDATQRRRPALVLSPAAYSARVGLALV
                     CPIASEPKGYPFEVLLPPGLAVCGVVVADRVASIDWQACGARLAGAAP"
BASE COUNT         3268 a         8123 c         8924 g         3117 t
ORIGIN      
        1 gccgccggcg ccctcacgaa acgcacacgc agagcagacc agatgggcaa gcccccggga
       61 cggcctggga cttctgacag cgacacagtg gaggccgtat agatggcaaa gatcctcacg
      121 agaatgggtg acaactcgaa cgtcgagttc accatggacg aactccgcca ggagttcatc
      181 gacgggtcgg agttcgcggc caagaaggcc aaggttccgc cgctgaccga gaacgagatc
      241 gactggctcg tcgacatgtt cgccgccccg acacgcatct ggggcgtgga gcgtggccac
      301 gaggtcgtca tgaccaagga cgggtgcgtc aacgcgctga actcgtcgca gatgtccagc
      361 ggtgtcgtgg ctccggtcaa ccgcgaggtc ggcgtgcggc tctacgagtc gatgctgggc
      421 ttcgactcca tggagatctc gcacaacgac tactcggtca agccgcagcg cttcctcaag
      481 aagctcgaga tgctccacgt cgagatcctg caggagacca cgatcctccc gctgctctac
      541 gggttcatgc cgaacctggg cctgtacttc aagaccgacg gtcccttcga caacccctac
      601 gacctcatgc agcagggcaa gatcaaggag tcgcaggaga cgcaggaggc ggccggcgcc
      661 aagtgcctcg aagactgcat cgagatgagc gactgcatgg tcgagatggg cgccgacggc
      721 atcgactacg acaccgtggc gtccaccggc gacggtgagt tcgcggcggt actcaagagc
      781 accgagcacg tcgccaagga gaccggcatg ccggtcgaga tcggcatgtc gaccgagatg
      841 gtgaccggca tccacggcac catcgagtac aacggcaaga gattggccgg catgtggccg
      901 caccagcagc tgcaggtgtg cgaggaggcc ggcgtcgcga tcttcggccc ggtcagcacc
      961 accaacacca agaagtccac gccgtggaac ctcggcaagg cgctcgtctt cgtcaagtcg
     1021 tgcaccgaca tcgccaaggt gccgatccac ccgaacgtgg gcatgggcgt cggcggcgtg
     1081 ccgatgttcg agaccccggc cgtcgacgtc gtgacccgct gcagcgccgc catgatcgag
     1141 atcggcaagg cagacggctt gtaggtgggt accggtgacc ccgccggcat gcacatggcg
     1201 catctgttgg cctcgtccat gggcggcatc agggcagcgg gagatctcgt tgctcgtatg
     1261 cagctcagca agaagatgag gatcgacgaa gccaagaagt acgtcgccga caagctccac
     1321 gtgaccccgc tggaactcag cgatccctac accatgcggc ttctcaggga ggagctcgac
     1381 atcgggacga tcacgggtgt gccgggcgtc gccaagggca tcgcggccaa ggctcgtatc
     1441 gcgcagctgc tcgacatcga gatcaactgc gtcgagcagt tcaagaagaa gaccggactg
     1501 cactactgag tctgtgagcg ggccggtcgg ccgcgcgagc ggccggccgg cccgtcattc
     1561 tgtcgtgaga cgagaggacg tcgtggacca ggaccgccgg gacctcgtga tgttcgactt
     1621 cgacggcgtg atcgtcgact cgctcgacgt gttcggcgcc gcgttcctcg acgcctgcgc
     1681 ggcgaccgga gtggaggggt tcacccggac ggacgacctg ctggcggtga tggagagcaa
     1741 cttcttcacc ggcatgcgcg cacgcgggat cgacgacgag cgggtcgcgg aggtcttgcg
     1801 ccgggtcggc gacgcgctga tccgcgcccg gcactggctc aagccgttcc cgctggtccc
     1861 cgacgtgctc gaggacctgg cggacacgcg caccgtcgtc gtcgtcacgt cgagtcccgg
     1921 agaggtcgtc gcgggctggc tgcgcacgca ccaggtgcac ggcgtgaccg aggtcgccgg
     1981 ggcggagtcc gcgcagagca aggtcgagaa gatccatgct ctcctggccc ggttccccgg
     2041 ccaggaggtc tactggtacg tcggcgacac ggccggcgac atccgcgagg cgcgtgaggc
     2101 cggcgtgacg ccgctgggcg tcgcctgggg ctggcacgag ccggagatgc tcctggaggc
     2161 cggcgcggaa cgcatcgtcg cctcgccggc cgaactgctc gcggtcgtcg cgccggagca
     2221 ggcccgggac ttcttcggcg ccggctgacc ggcgcggacg cctgccgcag cggtcagcct
     2281 tcgcggcgcc ccagtgccgc ggcggcggcg accagctcct ccgccgcctc ctcgccaaag
     2341 ccgccggcgt cgtggaacgc gaccaccccg gcgccgtcca gcagcaccac ctgcgtgcgc
     2401 tgaccgccgc cgtccccgat gaacgcgcgt gcggcggcgc cttcctcgta cagtgtcacg
     2461 acgctccccc agagggacgg cggcacgccg ccgcgcatgc cgccgtcgat ccagccctgc
     2521 agcggcctcc acagccgcga ggagatgatc ggcagctcgc ggacggcgag gccgggcagc
     2581 ctgcgctctg cgaaagccgc ccagcggtcg acgtcggcct gggtgccgcg ccggtacgcg
     2641 cagagcagca gcaccggcgc gccggcgaag tcctcgggca cgtgcacgag ctcgccggag
     2701 agcgccgtgc cggctacgga cgggaagcgc cggcccacga tgcgcggcgc cccggtcaac
     2761 gggcgcctct cttgccggcg gcgccggcgg tggcgcgccg ggggagcgtg gccgccacca
     2821 tctagcgccg ccgcgccggc cggaaggcgg cgaggtctcc ggggcgaggg acctcgtcgt
     2881 ccgccgcggc tccggcatct ccggcggcac gccacgacgc gcgtccgtcg ccaccgccgc
     2941 ccaggccgcg cagtccgtag cggccctgcc cgaggtcgct gatgatcggt gagtaggcga
     3001 tgcggttgtt gaagctggag agcttcatgc cggcggccac acacgcacgt tcgagctcgt
     3061 gccggtcggc ctcgcctccg gcgcgcatca aggcgcgcag cagcgtgagc tcggtggtct
     3121 ccaggacctc ctcgggagcc tgcggctggt ccgcgatcac caggctgccg gagacggtga
     3181 cgcccgggac gcgcgcgcag agctccgcga ggatgtactc gggcatcacg aactcgcgca
     3241 tgcgctcgtc ccggcggatg cccgcatgga ggtcggcgag gctgaccgcc ccgccgacgc
     3301 tgagcacctt ggcgaggcgt gagacgacgg cgttcctggc gaccgacggc agggagaacc
     3361 agccggtgcg ccgttcgagc caggcgaagt cggccggctc ggagacgacg gcggtgacga
     3421 gctcgtcgtc gacccacacc gcgaggtcgg cggcgacgcg ccccgtgacc tcgccgacgc
     3481 gggccgcgcc ggcgcgacgc accaggccgc gggcggtgtc ggcgatggcg gcgatgacgt
     3541 ggacggtgtc gttctcggga tcgggcgggg agggcagggc gacgcggacg cccttgacgg
     3601 actcgagggt gaacgtggcg tcgcgtccga gcaccgcggc ggcggtgagc acgcccgcgg
     3661 ggtcgaacgg ctgcgacgcg atgcgcgcgt cggccaggcg ccgggcgagg tcgtgcgccg
     3721 tggtcggcgc ggcgtgggcg gcgagcacga gggcccgctc gagcgccggc gcggcgacgc
     3781 gcggctccgc cagccgcccg gcgaggcgct cgcagagctg acgcacccgc tcgcgcgtga
     3841 tcccgcccag ctcgcgtccg gtcgtctcga gcgtggcgcc gccacgtccg tcccagcccc
     3901 agtggcgcac ggcgaggcgc gccgtgcggc cgggctcggc gtgttcggcg atctcggcca
     3961 gttcggcctc gaggggacga tcagacagct ccacgggcac tccgctccac gctgctcacc
     4021 tggggaagag gatacaccgt cgggtctgcg cgcactcccg cgtctcgagc gcctgcacta
     4081 tgatgtggcg gaccgctcac gtctgcgtcc ggggggagct cgatggccga caagcagaag
     4141 gacaggaaga aggccgaggc tccggagaag ggcgggccgc tgccgagcat gagcacgccg
     4201 ccggcggaga agcccggcca ccacaagaag ctcgaccgcg agcagtacga ggccgagctc
     4261 gagaagctcc aggtcgagct cgtcaagctg caggagtgga tcaagcacgc cgggctacgc
     4321 gtcgcggtga tcttcgaggg ccgcgacgcc gccggcaagg gcggcaccat caagcgcgtg
     4381 acggagcacc tcaatccacg cgtcgtcagc atcgccgccc tggccacgcc gacggagcgc
     4441 gagcgcacgg agtggtactt ccagcggtac gtggagcacc tgccgtccgc cggcgagatg
     4501 gtcctcttcg accgctcgtg gtacaaccgc cccggcgtcg agcgcgtgat gggcttctgc
     4561 accgaggacg aggtctggga gttcttccgc acctgccccg agttcgagcg catgctcatc
     4621 cgcagcggga tcatcctgct caagtactgg ttcagcgtga gcgacgagga gcaggagaag
     4681 cggttccagg cacgcctcaa ggatcccctc aaagtctgga agctcagccc gatggacctc
     4741 gagagccgca tgcgctgggt ggagtactcg caggccaagg acgagatgtt cgcctacacc
     4801 gacaccaagg actcgccctg gtgggtggtc aacgccgacg acaagcggcg ggcgcggctg
     4861 aacatgatcc gccacctgct cagccagatc ccgtacgagg acgtgaccca caaggacctc
     4921 aagttggccg agcggccgcc tgacaagggg tacgtgcggc cgccgatcga cagccagacc
     4981 ttcgtgccgg agatctactg agggcgaggg cgcggccggc gcgcgccggc ggcgtcagcg
     5041 caccgtgacg acggtgtggg cggtggccgc ctcgcggttg ccgcccaggt cgacggcgcg
     5101 gtaggtcacc gtgtacacgc cgcgcgcccg cggtcgccag gagatgctct gcgcagtgcc
     5161 cgtcgggcgc cagccgggat gacctgaggc cacccgccgg cccttggcgt ctgtgatcgc
     5221 gtacctcacg tcggccttgg cgctgaacgg gtccgtgacc ttgaagccga gcttggcggc
     5281 ggtcccgacg gaggtcgtcc tgccgccggc cgcgtagagc cgcggccggt acaggtcgac
     5341 caccagcccc ttggtcacgc ggctctctcc cagcgtggac acggcggtca ccgtgaacgt
     5401 gggccgggag ctggcgaggt cctcacccga ctctgcccgc ccgtcccagg tggccgagtg
     5461 ggcgccggcg gcgagcgcgc ccagcgacag cgtgcgcacg acgacggcgt cgagcaggac
     5521 gcgcacggtg acggtggcct tgcgcgtcag cttgaaggtg agggccgtcg cgtcgcggac
     5581 gccgttcccg tcgggggaga gggtgacggg cacggcggcc gggaagccga tggtgcgatc
     5641 gacagagatc ctgacgccct gccgggcgat gttgcccgcg gcgtcgcggc gctcgacggt
     5701 gaagcgatac tgcccgtcgg gcgcggccac gaggccgccg ccggaggtga tgcggccgtc
     5761 ccagccgacc gagtacgacc gggtctcgcg ggaccgccag ccgtggagcc agcggagcgc
     5821 gtcgccttgc gcgtcgagga tgccgacgcg gaccgcacag gcctccgcag gggtgtaggc
     5881 gaccgcggcg gactcggcct gcccgtcgcc gttggggctg aacgagcctg tggacgaggc
     5941 tccactgagc cggggcgcga ccgtgtcgac ggtgatctgc acgctcgtca cggccggctc
     6001 gccgccctcc ggtgtggctg tgagctcggc ggtgtagacg ccgtcgggga cgcgcgctcc
     6061 gccggaggcg ccgttccacg tgatggcggc gctcgtgccc tggccgctcc acgaggcgac
     6121 cgcctggccg ccggcggcgc gcacggcgac gcgccagtcg gcgggagcgc tgacgcccac
     6181 gtcgagcacg gtggtgtcga gcacgccgtc gccgttgggg ctgatgagcg gcgtgctcgc
     6241 acggagcgtg gccacgaggg cgctcccgac acgcttcgcc acgttggcgc ggacggacgg
     6301 gaggagcccg tacagggccg cgcccgggca ctcggtgtag ttcgcctggc ggtgcccggc
     6361 gatgaccggg aacgcgaccg tggcgccggc ggcgtacttg tccccggcgc cgcaggtgag
     6421 cctggccgcg ccctgagggt cgagcccgtg gacggccagc ttccaggcca gcagtcgctg
     6481 cagcgccgcc accgcctcgg ccggcggcgc ctcgtcgatg aaggtcccca tgacggagac
     6541 gccggtgctg ccggtgttga agccgtacac gtgtgccccg gcgaccccgc gggacacgcc
     6601 gccgtatcgt ccctcgtaga cgttgccgaa gcggtcgacg aggaagttgt aggcgatgtc
     6661 gttccagtgc agggacttgg tgtggtaggc gtagatgccg cgcacgagcg cgggagcgtc
     6721 gtcgcgcgag tagacgttgc cgctcgcggt gtggtgcacg aacgccatct tcacaggagc
     6781 gtacgacggc gccgcgttgc gcagggactc gtcggcgccc cactccgagc gcgtgacgat
     6841 gacgggggcg gcggcggccg cggaggccgg tgagtcgagg ctgacgccgg ccacggtggc
     6901 ggcgaagcgc cgggctgcgc ccccgacgcg cgcggcgatg cctccggact cggtggggtc
     6961 gatggcgacg agacgcacgc cggtgagcgc cgccggcccg ccccgggcgc ccgtcgccgc
     7021 cgcgacttgc gcgaagcggg cggggcccgt ccagagggcg tcggtgaagg cggtggccac
     7081 cgcgccgtcg ccgccgagct cgagcggcgc ctccagccac ggcccccagg cggagccgtc
     7141 caggctggtg cgcaggcgca gggtcactgc gccggtggcg gggacgtcgc aggtcacgcc
     7201 ggccatggtg aactccatgc cggcgtcgag agtgaccggg gcgccggcgc ctcgggcgga
     7261 gggcccggcg acgacgaggc gcccgtcgtc cgatggtgag acggagtgca ggcgcacgga
     7321 cggtgtccgg atccagtccg ccgccgcgcc gctcgcccgc ggcgcgaccc agacggcgac
     7381 gagcagcgcg gccactgcga cgcagacggt cgcgagggcg cgctcgaggc gtggatgccg
     7441 aaccgtatga gggcgagtcg cgtggacgcc ggtctcccgc agtcgcttgc tcctgctcca
     7501 gaacgccggc ggcgggcgga ccccggcccg gcgggggaac aggccttgcc ttcacggggt
     7561 cggccggccg cgtcggttcc ttgagcaaga agtcggctca ggcaggcgat tacgcctgcc
     7621 tggcctgctg cgggatgcct gcgaagcctg ctagtcggtg ccctcttcgc cgccgaaggg
     7681 ctcgccgacc aggggcaggt ccgggaggtc gtcgaggtag ccggcgacgg cccgtgccgc
     7741 ggcgtccacc aggtcggcgt ccgagagcgc cgcctcgagg gccgggccgc cggccgccgc
     7801 gagggccgcc gcgagctgca cgcagaggtg gacgaaggcg gcgggggacg gcgctcgcgc
     7861 cgtgccgagc tcgagggccg ggaccgccgt gacccagacg agctcgtcgt ccacggccac
     7921 ggccgtcacc ggccggcgac gcgggccggg caccgggacg cacccggcga gcgggagcag
     7981 cgagccaccg ccggtggaag agaggcggcg cccctccgcg gaggggccgg agagagcgtc
     8041 tggactcggc attgcggctc ctcggaggtc gctgtggatc gggcgttcgc gggcgagagt
     8101 agcagccggg gcggacagat gttcgtgtgg ggggaggttc ccattccgcc ggttccggct
     8161 aagatacctc accatcccgt gaccacagcc aagcgaagga gtcgccatgt cggacagcgt
     8221 ctaccgggtg atggaagtca tcggctcgag cagcgaatcg tgggagcagg ctgcgcggaa
     8281 cgccgtggag aaggccggca tccatcgtga ggacctgcgc atcgccgagg ccgtcgagtt
     8341 cgacatgaag atagagggca agcagatcgt ctcgtaccgg acgaaggtta agatctcgta
     8401 caagtatcac gagatcgtct gagaggggcg gccgttactc gagcccgagg cgcgcgagct
     8461 gctccgccag tccgccgggc tcgaggtgga gcatggcggc ggcgccttcc aggctcagcc
     8521 ggccgccgcg gaccgccgag agcgcccagg cgcgcatggc ggccggcacg cgcagcaccg
     8581 ccggccttcc atactctccc gccgggagcg cctcgtccgg ggtgaggtgc gtcagggtgt
     8641 ctcggagacc gcccaggtag gcttggcgcg gcagcagttc ccactcgtgc cgccgcagtt
     8701 cagagcgcag cacgtggaac tgcttgccct gcagccgtcc ggccgcccgg gaacggtaca
     8761 gcgccgccca ggcgctgatg ccgaacgcgt tgcccagatg aaggagatcg tcgacaccgg
     8821 tcacgcgcga cgcggcgcgg cggtcgtacc agcgctggac cgcgcggacg ggcgcgagca
     8881 gttcctctgc gaagtcgttg gcggccgcct ccggcggcgc gttgcggctc cactcgatgt
     8941 gatggtcgac cacgtcgccg tgtccgagga ccaggtgggc gaaggcgtgg gcgagcgcga
     9001 aacgctgcag gacgaccggc ctggcggcgt tgacgaagac gaacgggaca ccggcgtccg
     9061 cgaaagcggc cgcgacgggc gtctccgcgg ccggcggggt gtcggcgccc gagacgggtg
     9121 cggtcgcgcc ggccacgcga gcaacgatca cctgcgcgcc gtacccgtcc tcgagcagcg
     9181 gcacgaggtc ggggagcggc ccgtcgtcgg ggtcgacgtg cagctcactg cgccagaagc
     9241 gggcgacgcg gtcgtagagc acgtcggcgt ccggtgctcc cccgcgcggg gaggcggcgc
     9301 tgtgcgcgac gcgcccgggc ttgccctccg ccgagtgtcc ggtgctccag cgctgcgccg
     9361 gctcgggatc cttggccagc tcgtgcaggg cgaaccagtc gcgcgccacc ccggccactt
     9421 cgccgggcag tccggcgaga ccaggacgac cgaacggagc gtcggggcct gagacgtagg
     9481 agcggaaggc atgacgggga gccggccgga ccacgcgctc gagggccgcg tgttcgggcg
     9541 ccgcctgcga ggcgtgcatc aggtgtgcgc gcgccgagga aggcggcccg gctgccgacg
     9601 cggccgcgtc gtcggtcacg agggagtgga tcgccggcgc ggctccgagg agctcgtcgg
     9661 ccgagacgtg cagcgcgtcc gcgaaacgct gcagctcatc cgcggagagg cgtcgccggc
     9721 ccgcctcgat gcggctcacg gcggactggt cgatgccgac ccacttcgcc agggcgcgct
     9781 ggctccatcc ctgctgctcg cgcaggctcg cgatcagcgc gccgatgctc gtctgatgtt
     9841 cgtcgcgcat cgtttccgtc acctcctcct tctacactct gcatcagtct acgtgaagac
     9901 atgccccgtt tcctgcatcg agcgaacgtg acaggtcttc aggatgatgt gctattgtca
     9961 tcatcatatg gtactttacc gaagactgtg atgccgctga ggcatcgtag tccatcagga
    10021 gggcgccgct cgtcggccgg gccgggccgg cgggcggcca cggagacgga ggtgtgtcgt
    10081 gacgaagaac aacgaggtgc ccggtggcgc cgccctggtg gaggcgctgc gactggggga
    10141 gcgtcaggcc cacgggccgc tggcggtgtt cccgctgcgg ctcggccccg gcgacggccc
    10201 cggctacatc accctgcgtg aggccatcgg ccggcagcag gcgacggtca ccgaggtgag
    10261 cgatggcggc tcggtgccgg agctgcgcgt cgtcaacaag ggggacgagc gcgtcctcgt
    10321 gctcgacggc gaggagctgc gcggcgccaa gcagaaccgc gtcctcaaca cgaccatcct
    10381 cataggcggg cacagcagcc tggtcgtccc ggtcagctgc accgagcagg ggcgctggtc
    10441 ctacgcgtcg cgtgagttcg aggagagcga gatcgtggcc gaccgtcagg tgcgctacgc
    10501 gatgaaggag agcatcaacg cttcgctggt ggcgggcgcc ggtcacaggt cggatcaggg
    10561 ccgggtgtgg cacgaggtcg gcgagctgca cggccggcac gccaccacct cgccgacggg
    10621 cgccatgcgc gacgcctacc agatgcgcaa gggagacctc gacgccgtgc tggctgcctt
    10681 cccgctgcag gacggccagc agggcatgct ggtgctgcac ggcgcccgcg tggtcggcct
    10741 cgacctcgtc tcgcgggcgc cgcagtacgc cgagctgcac gacaagttgc tgcgcagcta
    10801 cgcgttcgag gcgctggtgc gtgacggcga gccgggcgac agggccgtcg ccgacgcctt
    10861 tctggagcgc atcgccgacc tgcccgggag ccggtacaag agcccgggcc tcggctggga
    10921 cgtgcgctac gaaggcggcg gcgtgctcgg ctccgccctc acctaccgcg gacacgcggt
    10981 gcacgccgcg ttcttcgatg tcggcggcgt acgcggggcc tcggatgacg gcggctccgg
    11041 cggagacccg cgtggcgcga gccggccgcg cccggactgg cgcatcgccg acgcacgcga
    11101 gcgcgcccgc aggcgccagg cccactgaag gaggagacca tgacagaaga cacccgtcag
    11161 cgcgcctaca aggcggccgc ccacatcacc cgcgagcagc tcaggcgtga cgatcccgag
    11221 cgcgccgtca tcctcgagca ggtcgagagc agcgacgtga tcgtggtcgc cggcatctac
    11281 gaccacgtcg agtcggtact cggcgcgctc gacgtgccgc acctggtggt gcagccgcac
    11341 gagctcgatc agctggcgct gcgccccgag cagctgctca tcgtcaactg cccgggccag
    11401 atcgcgccgg cggcggtccg ccgcgtccgc gccttcgtcg aggccggcgg cagcctgttc
    11461 accaccgact gggcgctcaa gcacgtgatc gagccggcgt tccccggtgt gctggccttc
    11521 gacacggcgc cgacgcggga cgacgtggtg cgcatcgagg tgctcgacgg cgacaacatc
    11581 tacctgcagg gcgtgctcga cggccaggac gacccgcagt ggtggctcga ggcctcgtcg
    11641 taccccatca gcgtggtcga cgaggcgcgg gtcgaggtgc tcatcaccag ccgcgagctc
    11701 ggcgagcgct acggcgagcg tccggtcgcg gtgtggttcc gctggggcga gggcgacgtg
    11761 ttccacatga tcagccacta ctacctgcag cgcacggagg agcgcagcgc gcgtcacgcg
    11821 gcccctgccg gcgcctactt cgccgagaag ggcgtgccga tgggccagga gatgtgtcag
    11881 gacgtggccg acctcagcgt ggccgacgtg gagtcggcca agccgtccac cacgttcatg
    11941 agcaacgtga tcatcgagaa gaagcgccgg caggcgaggg aggggtgatg agggtcacca
    12001 ccgacctgcg gccgggcatc gcggtggagt tcgaacaggt acagggcgtg ccgatggcgt
    12061 gggtgaggct gccggatctc gccgacgtcc cggacgatga tctgcgcagg gcgtgcaggc
    12121 acgcggtgaa cgaggcgttc gtggcccagg gggccgagcg aggctcggtc ctcgctcgcg
    12181 ccctgaagcc tctcgacgaa gagctgcgtc gccgcggcct ggagccggac ggctggccgg
    12241 tggtcggccc tgatccggcg tgcgcgccgt ccgccgcgtg gggtgagggg ccgggacgag
    12301 aggacgacgt ccccccgccg cgccagggac ttcccagtct ccgcggtctc gacgaggcgc
    12361 agttgcgaca ggcccacctc tgcgcgatgc gccgggtctt ccacgccacc gggccggcat
    12421 gcatcggcat gcgtgcgacc ctgctcgagc ccgtcgaggc cgagatgcgc cgccgcggcc
    12481 tcgcggcccg ctactggtcc gcgctcgatc gctcgcgccg ggccgccgcc ttctggtccg
    12541 cgctgaactg aacgaggctg atccggaact gggagtcctg ctccagtctc cgagtccagt
    12601 cgcgcgagta ctgtgctcac agtatgctcc ccggacaacg gtggcccgag gggccggatg
    12661 ccggcatccg gccgctcctc gccgctgtgc ctggggtagg ctggaggcca tgtccgcctg
    12721 gtcgccgctt cggtaccgcg tctaccgcta tctctggatc gccctgctcg tgtcgaacat
    12781 cggcacctgg atgcaggccg tcggcgccgc gtggctgatg gtggaactgg atgcgtcgcc
    12841 ggccgtcgtg gccctggtgc agacggcgac gtatctgccg gtggtggtgc tcggcgtgct
    12901 ggccggcgcc gccgccgacc tcatcgagcg gcgccggctc ctgctcgtga cgcagatgat
    12961 catgctgtgc gccgccggcg ccctggccgt gctctcgggg ctcggcctgg tgaccccgtt
    13021 ctggctgctg gtgctgacct tcgcgctcgg cgtcggagcc acgctgaacg cccccgcctg
    13081 gcaggcgatc cagcccgaga tcgtgccgcc ccaggagctc aagcaggcgg tcacgctggg
    13141 cggcgccaac atcaacctcg gccgggccat cggcccggcc gtcggcggcc tgctgatcgt
    13201 cgcggcgggg ccgtggctgg tgttcgcgct caatgccgcg tcgttcgcgt tcgtgctcgc
    13261 cgtgctgtgg cgctggaagc gcccggtcga ggagcaggtc ggaccgcccg agcgctttgc
    13321 cggggcggtc cgtgccggca tccgctacgc gatgttctcg cacatcctgc acggcgtact
    13381 ggtgcgctcg ttcgccttcg gggtcgccag ctcgggcttc atgtcgctgc tcccggtgta
    13441 cgccagcgag gagctcggct ggggatccgg cggtctcggc atcctgttcg ccgcgatggg
    13501 actcggggcg gtactgacca cggccgtcgt cccgtcactg cgtgagcggc tcagcgccga
    13561 cggcgtgttc gcggtgggca gcgcgctggt cgcggccggg ctggtgacgc tggcgctgct
    13621 gcgcgcgccc acgccggcgg tggtcgcgtc gttcgtgatc ggcaccgggt ggctgtgctg
    13681 cctctcgacg ctcaacgtcg cctcgcagga ggtgctgccc ggctgggtgc gtgcccgggg
    13741 gctcgcgctc tacctcacgg cgatctccgc cggcatcgcg ctcggcagcg ctctgtgggg
    13801 caagctggcc aacggtaccg gggcccccgc ggcctacgcc tggggtgcgc tggcgatcgt
    13861 ggtcaccgtc gggctggcgt ggcgctggcg gttcgaccgg atcgcggatg tggacctgcg
    13921 cccggcgccg atggccgccc cggaggcgcg gctggtcgca ggccaggacg ccgacagccc
    13981 ggccctggtg gtggtggcct acgaagtgcg ggaggacgcc gaggacgact ttctccgcgc
    14041 cctgcgcctg gtaggccggg tgcggcgtcg tacgggggcg accgactggt cggtctaccg
    14101 cgacgcggac aagccgcgcc gcttcatcga gaccttcgtg ctgcccagct ggcaggagca
    14161 cctgcgccag caccaacggc gcacggtgac cgacctggaa ctgcaggagg acgtgcgcca
    14221 gtacctgcgt ccgggcgccg agccgacggc gaagcacttc gtggcgccgc cggagccgtc
    14281 catgcgcttc tggtcgcggt aaggcgggct cacggcggcg aagggccggc cgctcccggc
    14341 gccgcgtcct cctcctgcgg cacgagctcc gggaacagca cctcgatctc ggcgagccgg
    14401 ccggcgatga gctcgcgcag ctcggcgccg ggctcttcgg cgagccaggc gctcagcgcc
    14461 tcgtgctcgc cggggtccgg cgcctccacc agctcccgca ggaactggtg gacgaagccc
    14521 gaggtgctgt cgaccgtgaa cagcgccgcg tcgcgagccc gcgtgacgcc cacgtacacg
    14581 acgcggcgct cctcctccac ctcggaagcg ctccagcggg acacgtcgca gtcgtagtcg
    14641 gggatgacga tcgagtcgta ctccctgccc ttggcggcgt ggatggtgcc gatgaccacg
    14701 cggtcctcct cggactcctc gcgcgcgagt gtgtcgtcgg ccaggccggc gtgtgcctcc
    14761 tcgtcggcgc gcaggcggtc ccacgtgcgc aggtactcgc ccgggtccgg gtaggtctcg
    14821 gcgaggagca aggccgagtc gaagacctcc agtgggccgg cgccgtcgga gcccgcgccg
    14881 tcgccggccg cggcggcatc ggcctccgcg gcccagaggt cgtcgagcgt gaatgcctcg
    14941 acgaccgtcc agacgaggtc ctcggaggtg aggacctcgg ccgtcgcgag ctccgggccg
    15001 aggcggtcga cgagctcgac gagggcggag agcgggcgcg gcccggccgg aggctcctgg
    15061 gctccgatcg ctcgcagcct cgcccacggc cgcgaggccg cgccgatggc ctccacgacc
    15121 gcggcgctca ggtagcggtt cggccggttg agcagcccgg cgagtgccgg tcccggcagc
    15181 tcgtccggag cgcgcaccag gtccaggtac gcgcgcagga gggtggccgc cgggtgcgtg
    15241 aacagcgtgc agccgagcgc cggtgtccgc ggcacgtcgc cggcgtcgag cgcgagcgcg
    15301 accaggagct gctgcgagcg gtaacggcag agcacggcga ggtcgcgcca gtcgcagccg
    15361 agccgctgct tctcggcgtg caggaacgcg cagatcgtcg cagcgcgctc cggccaggag
    15421 gccgcgccga agaagcggac ggcgccgtcc tgcgctccct ctcgagggcg gatgtccttg
    15481 gcctcgcgga cgaggttgcg cgcgaccagg cgagcgccgg tctccacgac cgcacgcgag
    15541 cagcggtagt tggtgcacag cgtgtacgtg gccgaccagg gcctgggcgg catccggcgg
    15601 tggaactcga ggatgccgcg cgggtcggcc tgccgccagc cgtagatgag ctggtcgtcg
    15661 tcgccgacca cgaacagccg ccggtgcggg cgggagagca cgtccaccag cgcgagctgc
    15721 gcgccgttca ggtcctggaa ctcgtcgacc aggacgtggt cgaagcgcgc ctggatgaac
    15781 gcgcggtggc tcggctcggc gagcatgtcc gcgaccgcga agtagatctg gtcgtcgaac
    15841 gactggagcc cggtagcggc ctgcgcacgg gcgtactgcg cgtggatggg ggcgaagggc
    15901 aggtccagca gcggcgtgtc gccgagggac tcgatgcgga cctcgacgcc ggccgggggc
    15961 tcgagcgccg cgcgcacgcg cgtgaggccc tgcatgaagg cgccgaccgg gtcgctgccg
    16021 cggcgcggct tgagctcccg cagggagacg ccggcggact ccatggcgcg cgccatgagg
    16081 gcgcggagcg agcggccgtc gtggtcgagc gagaaacgcg cgcgcagcac ctcgcgctgg
    16141 tagcggtaac cgaaggcgtt gaaggtggca cagtggacgc cggacgggcg gccggggtgg
    16201 ccgggcggtc cgccgcggcg gcgcgggcgg cgcgagggcg acgcctcctc cagcggcgac
    16261 ccgaggcggc gcgtgctcgc gatgccgagc gccgccagcc gctcctcgag ctgctccgcg
    16321 gccttgcggt tgaaggcgag catgaggatc ccggacgggt ccgcgccgcg gtccacgagc
    16381 tcgacgacgc ggctgatgag cgtcttggtc ttgcccgagc ccgcgggcgc gaggacgcgg
    16441 gccggcccgc gggcgtgctc gacggcggcc cgctgcgccg cgtcgagctg gaccgcgtcg
    16501 gggccgctcg aggggcggcc gtcgcgggtg gggagcgggg cggtgggcag gccggcggcg
    16561 ggaaggccga cgacggccgg ctctgaagac ccggcagcgg cgcgggcagg agggtctccg
    16621 cgcccggccg tcgccgcccg caggagccgg ccgatgtcct ccaggtcgag ccgcgcggca
    16681 tcgccgacgg cgagacggcg cggctcgagc acgtcctccg cgaacgggag accgaggcag
    16741 aacgggaggc cgcgggcgac gagagggtgg acgaggcgca gaaggatctc cgtcgcctgc
    16801 gcctcgaact cgtcgcgcga ccccttccag taccagagtc cgaccgggac ctcgccgccc
    16861 aggagggggc gacggggctc gccgggatcc tcggtccacg cgcgccgtga ggcgaagcgc
    16921 gcgcctgggg cggccgccgc agctcgctgc gcgaggtcgc ggcggctcgg gacgtcgaag
    16981 aacagcaggc gcggcccgct gcggtcaccg tcggcgcatg ccgctgcggc tgcgtcgcgt
    17041 gtggcgaggg gcggcgcggg gcggtcggca tcgaggtaca tgggcgagga cggtagcagg
    17101 cctcgcccga gaggacgagg gtgacgaggg tggacgttga cgcgccccgg gcggcgccgg
    17161 gcgtctctac ttcagcgccg cgtacagccg atcggcgaac ccgatctggt cgatcgcctc
    17221 cgggtgccgc aactgccgcg ggtcggcgcg ctcgccgaag ccgagacgga cgcggtcgag
    17281 cgcgtcggcg tccttgagca gccagaggat gcggagggcg cgctccggcc gtgccgggca
    17341 gcgcgccggg tcttcggcca cggcgagctc ggccgcttgt gccgcggcgc ccccgtccgg
    17401 tagcgagtgc cgggtgatgg cgaagcggac cagggcggcg tcgtccggag cgaggccggc
    17461 ggtgagcccg agctcgtcgg cgcgagcggc gccgcgggcc ccgtgcggag gttccacgcc
    17521 gtcggtctcg cggccgatgt cgtgccagag agcggcccga aggacgagcc gcgcatcagc
    17581 ctcgtcccag cgcaggcgct cgagcaggcg ctgcgcgtgc acgtgtacgc gccgcgtgtg
    17641 gttctcgccg tggagccgcg acgcacgccg gaaccaccac gcgggtacgt ccggcgcgcg
    17701 tgcgacccac ggcgccacgc gctcgatccg gggagtcgtc atggaggcag catagcgcgc
    17761 cggccggacg agaccggcgg cggcgcggtc cccggctcag agccactcca cctgcttcat
    17821 gaacgcgtcg tcgcacgtgg agcaacggaa cttctcgtcg ggcagatcct cgtggtgact
    17881 cgccttgatg tggtgctcga gggacttgag tgaatcgaac tcggccgggc aatgggggca
    17941 cgggtgcatc tcgaggctca ctgtgacctt cctttcatcg gcaagcgttg gggcgcggac
    18001 gtcgagatac ccaaggggcc gccgtcgaaa tctgaccgtg ggggcaggca atggtgagac
    18061 gcggccgcgg cagcaaccgg gggctgcggc gttcccgtct ggtactctca gaccatggat
    18121 gcgcacccgc cggacatgac tctcgcggac cgtatgctgg tcgtcgaggg ggacatcacc
    18181 cgcctcgacg tcgacgcgat cgtgaacgcc gccaacagtt ccctgctcgg agggggcggt
    18241 gtggacggcg ccatccaccg tgccgccggg ccgggcctgc tggccgagtg tcgcggcctc
    18301 ggaggctgcg cgacgggtga ggcgcgcatc acccgaggct acgacctccc ggcgcggcac
    18361 gtgatccaca ccgtcgggcc cgtctaccgg cagcacgacc cggccgaggc tgagcgcctg
    18421 ctgcgttcgt gctacacgga gtcgctgcgg ctggcatgcg aggcgggact cgagaccgtc
    18481 gccttccccg gtatctcgac cggcgtctac gggtacccca aggccgaggc gtgcgacgcg
    18541 gccgtgcgcg ccgtggccgc gtggctcgcg gcgcacgcac tgccccgcgc cgtcaccttc
    18601 tgctgcttcg gcgcggagga tgcggcgctg caccgggcgc ggctcgggct gacgagctag
    18661 ccgcgcctcc cgacaccacg cgacgaagag gtggccaatg gcgagtcccc gctggagcga
    18721 cttcggcagc atctggaaga cgatccgcga ggtggacgtc ctcgccatcc gccgggaggc
    18781 ggagcacgac ctgaccgtcg cgtgcgcggg cgaccgtgag gcgctggagt accttcgcgg
    18841 cctgctgctg gagggacccg accgctatcc ctcgccgttc accgccctcg ggctggtccc
    18901 tctcgatcag gtgcacgcgc gcgagcgcct gatcagggag gcggacctgc tgctcgtcgc
    18961 gctgcgcgcc gacgtctctc tcagtcccgg cgaggtcgcc gggctggagc gcctcgagtc
    19021 gctctcaccg cgacgccggc tgctggtgct cgtgggcgag ccggcggctc cgtccaggag
    19081 ttggctgcgg cggctcgacg agggcaagac cctcgtcgtc cggcccgaag ccgccggctc
    19141 cccggaccgg ctcgccgccg cggtcctcga agccctgccg ccgcagcggc agctggcggc
    19201 cgcgcgccgc ctgccgggtc tgcggctcca gtacgcgcgg cggttgaccg ctgaggtgtc
    19261 cctctccaat ggagccttcg ccctcgcctc cggcgtgccg tcgctcgtgc cattcctggg
    19321 cgtgcccatc accgccgccg acaccgtggt gctcaccaag aaccaggcgc tgatggtcta
    19381 tcggctcgcg ctcgcattcg gtgcgccgcc cgagttccag cggcgcatga tggagatcac
    19441 cccggtgatc ggcgccgcgg tcgtctggcg tcaggtcgcc gggggactgg taggcctcgt
    19501 gcccggctac gggatcctgc ccaagaccgc ggtcgccttc gccgggacgt acatcaccgg
    19561 gcgggcggcg gagctgtggt acgccaccgg cctggtgtcg aaggacgaca tgcggcggat
    19621 ctccgccgag gcgacggcca aggccaggtc gatggccggc gagcaggcgc ggcgtgcccg
    19681 cgaggcgcgg gacgccgccg gccgggcgcg ccgggcgacg acccgcgtgc gcgagaacgc
    19741 ccggcggctg acccgccggc gtggcccgcg gaccacctcc gacgcccctc cggccggcca
    19801 gccggcgccg ggggccttcc tggatgcgcc gccggcgtcg cccgagaaag agtagcaact
    19861 gcgggtgccg gggggccagc cgggcccgag aagaacccgg cggcgttgaa cctggcgcat
    19921 caaggttcgc cgtgcgagtc ggcgagggac gctagccggc gagcaggccg cggcgggcgc
    19981 agacctccgc ggcttcccgc accagggctg cgcacagggg tgcgtccgcg gtctcgagcc
    20041 gcgcggcgat ggcctcgagc ccggcgaacg tcgaacgccc ggggcgcagc gcctcgtaca
    20101 ccgccagcac ctcggcgtcg ggcagcgccg tgagctctgc ggcgcggcgc aggttctcgc
    20161 ccagctgcgg gttgccgtgt tcctcagcga ccgcggcctg gtggcgcagc acgtcggggt
    20221 ggacgcgcag gtcggcgggg gagaggtcgc cgcggacggc ggcctcgagg gtgatctcct
    20281 cgactggacg gccgctgagg gcgcggggcg tgcgcgcgac gccgggcgtc accggcgcgt
    20341 ctccaggtcc acgggcggcg cgccgggcac cgactcgccg cgttccacgg cgatcaggga
    20401 gaccacctcg gcgtggtagc gcgcctcgat cgcctggtcg gtgtaggggt tgcggacggg
    20461 cgccggccgc agaccctttg cgtggcggcc ggcgttcgcg cccagctcgc gatacatcgc
    20521 cgcggtgagc agcggcgcga tcgagagcag ctccaggcac gccagcggcg cctggtcctt
    20581 gcggtggatc agcgccgtac cctttgcctg cagaccgacg ccgacgccgg agccggcgag
    20641 ctcggcggcg gtcagcccga tcaccccgag gtcgacggtg gaggagacgc gcacgacgcg
    20701 gggagtgcag ccctcggcgg cgatgccggc catgacctcg gccagcgcct cgcccaccgg
    20761 caggccggac atcgtgagcc agagcttggt cgcgaacgcg ggggagacgc cgatgcagac
    20821 ctcgcgcggg tcggcgccca cctgggctgg accgcgctcg accaggcgga acggctgtgg
    20881 gccggccgtg gccggcggcg cgccggccgc gtggcgcgcc agcaggtcgg cgcgtgagcg
    20941 ctgctggcgg atggcggcga tctcctcgcg gcgcgccgcc ggcacctcgt agccggtgcc
    21001 gggaccccgg tagtcgttgg ggtcggtgac cttggagagg cagtccatct gctcgtcgaa
    21061 gatcgccgcc gtctgcaggt agtcgccggc gacgcgggcg cggcccatgg ccaggacccg
    21121 ccgcgcctcc tcctcgaacc cggtagcgaa gagcgcccgg acgacgtcga ggatgccgat
    21181 ctgccgctcc atgatcgcgt cggacgccgc caggatcgcc tgcgggtctg gcggcgtgac
    21241 gtccttggag cccgcggcga cgacgacctc gcccacgtgg tcgtcggtga agcccgccgt
    21301 cggctcatcg cccggcgcga tcagccccaa ctcgcggtac accgccaggc acgccttcgc
    21361 cgcgcgttcg cgcacctccc tcatgcgctg ttccgtcacc ggccgcagcc cgccgtccac
    21421 gccccagtcg cgctgcagca cgaggtagtc atccaggtcc tcggcattga agttgctcgg
    21481 gccgaacatg ttgtcgtagc gcgggatgga gccgaagccg ctgaagagga agtccgagcc
    21541 ggcgaggaac agcgggacgg tgtgggcggt gcggcgcatg tcggactcgc tgaccagcgc
    21601 gtcgttgccg gagcacgact cgaggtccct gagcatgacg aggaggttct cggcgagcag
    21661 ctcgcgcatg ccgtcgggga cgcagccgac gacgctggcg ccgtcgatgc cgccgttctg
    21721 cacgccctgg gcgcccatgg cgcgggcgag cgcgacgcag cgcgactcga ggctgaacat
    21781 ggagcggccc tcggcggcgc ccatgagggc ctccgcgccg ccgccgctgg tcactcgcat
    21841 cttgagcccg cgggaggcgt aggcggaaca caggaacgcc ttggaccacg gcgtgtcgtc
    21901 gccgtcgatg aaggccttct cggtgccgta gaggctgacg gtctcggcgt aggtggtgag
    21961 tcccctgagg ccgagctcga gctcgagggc ctcctcgatg gagcattgcg tgagcgcgcc
    22021 gcaggcccct acctgcgagc cgatgagaac ggcgagcgcg ttggagggcg cgtcggcgtt
    22081 gacgggcacg gtcgtctcga cctcgcggaa gccgtaggcg accgcggtgg ccgcgtcggc
    22141 ggccagcagc agcgggtcgt cgagccggtt ggtgacgtgc gcctggttgc tgggcgtacg
    22201 gcgcacgcgc atcttggcga ccgccatctg gagctcgacc ggctgcagca gcgcgacgac
    22261 gcgagcgagc ttctcgggcg tggcgccggc ggcgaggcgg acgacctccg cgcgcggggt
    22321 gccggggtcg acgatcaggc gtgcgaaggc gatgtcatcg acggcgttcg cctccatcgc
    22381 gaccacgggg tcgaggccgc gcgcggcgat gaaggcgtcg atgaggtcgt agtccgcggc
    22441 cgcccgaccg tcgagctcga cgacgcgacc gtccgcgtcg agggcgacgg agggctcggg
    22501 atcgttcgcc gaggcgaacg cgacgagccc cgcctcgtcg tcgcgctggg cgaagccgtc
    22561 cagattgacc ggccgctcgt ccagcgcgtc gagccggccg aggcggggcg ggacatcgcc
    22621 ggctgccggg atgcgctcgt cgccgttcgt catgcccgga tactaccgag gcggcgcccg
    22681 tgctccaacg tccgcggcgt cgcgaatgcc aacctggggc cggccgccgg gggcttgtca
    22741 gggaccctgt cagggcgctc tcttctcgta gagacgttgt agagacgaaa tcggagaaga
    22801 cgccgagccc tgcctgtctt gtagagacat tgtagagacg cgttcagagg ccgggaaccg
    22861 gatgaggcca ccgggagccg gcggagtgga agggcaggca ctgaggagca catgatgaag
    22921 gcgaaggtcc agcggtgggg gaacagcctg gcggtcagga tccccatgtc cctcgcgcgg
    22981 gagctcgggc tccatgacca gtccgcggtc gcgctgcggc aggaccaggg caggatcgtg
    23041 atcgagctgc tgcgctcggc cccgaccctg gaggaactgc tcgcggaagt gacgcagcag
    23101 aacaagcaca cggacctcga ctggcgcccc gcgggcgggc tggagaccgg cgagagatga
    23161 agtactggcg gccctggacg ccggcgtgcg gcgacatcgt gtgggtccgg gacgcgacgc
    23221 aacggcggcg gccggccctc gtgctgtcgc cggccgcgta cagcgccagg gtggggctcg
    23281 ccctcgtgtg tccgatcgcc agcgagccca agggctaccc cttcgaggtc ctgttgccgc
    23341 ctggacttgc cgtgtgcgga gtcgtcgtcg cggaccgggt cgcgagcatc gactggcagg
    23401 catgcggcgc cagactggcc ggcgcggcgc cc
//
LOCUS       DASUMX010000183         5497 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1502018, whole genome shotgun sequence.
ACCESSION   DASUMX010000183 DASUMX010000000
VERSION     DASUMX010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 5497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5497
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1502018"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(129..1562)
                     /locus_tag="VFZ86_09090"
     CDS             complement(129..1562)
                     /locus_tag="VFZ86_09090"
                     /inference="COORDINATES: protein motif:HMM:NF024220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HEX5642477.1"
                     /translation="MSRKRKLALVFVAFAVLVIAAPALAADRTPQVVARAEAGATTYW
                     YTGTEQIYLVPAGTTAVTITAAGAVGGADWRNAKPGGGRAVVTATVPLPLGTTVLYVV
                     VGGPGSVPASDRSGAPGGFNGGGASIISWGGSGGGASDVRTQPISVALTDTDSRLVVA
                     GGGGGAGSGEGSGGTAGSAGFSAVTGAGAGGSGSDASATESAAGNGGLGGTMGATAGA
                     LAPSSTGIAAEAGQLGVGGAGSAGWYDFFMTNVYVTFAGGGGGGYFGGGGGGDGTYGG
                     GGGAGSSYWVESAVDTSMTRSAGNAYVSISPPSALPVITGFSPASGTVGTSVTLTGTG
                     FTGATSVKFGGTAATSFSVVGDTQITTAVPAGATSGTISVTAPGGTATSAGAFTVASP
                     RPVVTRLSPTAGKRGVTVTITGRAFGSKSRTSWVKFGTVKCGTYVSWSNTRIKVRVPV
                     RAAFGKRSVRVTTAGGTSNAKTFTVKR"
     gene            complement(1838..3187)
                     /locus_tag="VFZ86_09095"
     CDS             complement(1838..3187)
                     /locus_tag="VFZ86_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX5642478.1"
                     /translation="MKVVRRVEGAPAASGVGMGVRRGTGLLFLLFLACCAPAALAAAA
                     QPARAASISVQVRAADTLLPLTGIRADLYQSMPWGWGGDWRCGYSDPVSGGVRFDGLA
                     AGTYVLELHDPASAYASCCWSDAFTRAEATKIVLANDTAYAADVELLPAGRVAGVVTD
                     EYGTPLGGISIWVGMDGQAGTGRTAADGGFDIGGLLPGAAWKLYFEDTENEDYVYQWW
                     DGQRVFEDATPLSVTSGQTTTVAVKMLTSAHIDGTITTRFGLPARDVAAQAFRLSDTG
                     RWVDWSGECCHEDGSFRIFHLPAGTYIVAFWDTDRPSHKRYYPDTALRSEAKAFTLAA
                     GETIDLHEVVWNDTQRPTPAAPDAEIVDQDEMADVRMRVNDPRPFGPRADVTIKVRTR
                     GGRLIKTVVLPRRRVNTWLHGRFLCDLPRGRYRYSVHARDSGGNRETRPAVNTLRVR"
     gene            3221..3482
                     /locus_tag="VFZ86_09100"
                     /pseudo
     CDS             3221..3482
                     /locus_tag="VFZ86_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008051974.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
     gene            complement(3532..4518)
                     /locus_tag="VFZ86_09105"
     CDS             complement(3532..4518)
                     /locus_tag="VFZ86_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016878491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HEX5642479.1"
                     /translation="MSDGTQSNAEAGGTVSVTWENYPRANCHASFRANVGRVGVGRFY
                     HVRDLAPVDEGQLGIGENRDTLYSLGVFDLTEPVTITKPETSGRYQSMLVQNEDQYLK
                     LSTTGPGRYTITREQAGTRYIGVNFRTFVDPNDPADVREVQAIQDAIVVEQASAGSFE
                     IPDWDWESYRRIDGAFRVLYYTMDDWSNAFGDVGEVDPVKFYVASASGWTGVPEPHEA
                     LILQGSPPRNDGTTPHTLTVRDVPVDGFWSVTVYDRNLSLNKNDYDAYIVNSVTAKPN
                     DDGSITIHFGGDPDQPNFIYTPEGWCYLVRLYGTREAIADGSYRFPKPQPVG"
     gene            complement(4683..>5497)
                     /locus_tag="VFZ86_09110"
     CDS             complement(4683..>5497)
                     /locus_tag="VFZ86_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009110069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HEX5642480.1"
                     /translation="DRPATDFVDISGKYMNAIPPSDASLFESVATVVQEEPLEAVDPE
                     TRGLLAAVGIRKDAPFAPDARMQAILAEAAAVGNATARAVLFSPRDPELLYYPDGAWK
                     MGYKGDGTDFSPGGVLDLDARTFYFSITDQISPAMWVKMVGKASQYAWADHDAEGRYL
                     DGGRTYTLHLPPDIPVKDFWSLTVYDPQTRSMLQTDQRFPMVGSQRPGLQANADTSVD
                     VYFGPEPPAGNEANWLQTVPGKGWFLVLRLYGPLEPWFDKTWRPGEIELVEA"
BASE COUNT          788 a         1939 c         1892 g          878 t
ORIGIN      
        1 gacagcgcga tgacctcctc tctacatccg gaccgtcaag cagtcagcgc cgcacgcctg
       61 tgaccctcgg cggttttgcg cacgtccgcg gcgccggcgc tcaactcgtc tgcagtgcat
      121 tccgccggtt agcgcttcac cgtgaatgtc ttggcgttgc tcgtcccgcc ggcggtcgtc
      181 acgcgcacgg agcgcttccc gaaagcagcc ctcaccggca ccctgacctt gatgcgcgtg
      241 ttgctccatg agacgtaggt gccgcacttc accgttccga acttcaccca actcgtgcga
      301 cttttggagc cgaaggctct gccggtgatc gtcacggtga cgccgcgctt gcctgcggta
      361 ggcgacagcc tggtcaccac gggccgtgga gaggcgacgg tgaaggcccc tgcgctggtc
      421 gcggtgcctc caggagcggt cacgctgatc gtgccgctgg tcgcgccggc gggcaccgca
      481 gtcgtgatct gggtatcgcc gaccacgctg aagctcgtcg cggctgtgcc gccgaatttg
      541 accgacgtgg cgcctgtgaa gcccgtgccg gtcagggtca ccgaggtgcc caccgtaccc
      601 gaggcggggc tgaagcccgt gatcacgggc agcgctgacg gcggcgagat agagacgtac
      661 gcgttaccgg cgctccgggt catcgaggtg tccacggccg actcgaccca gtagctggac
      721 cccgctccgc cgcctccgcc gtaggtgccg tcgccccctc caccgccgcc gaagtagccg
      781 ccgcctccgc cacccgcgaa ggtcacgtag acgttggtca tgaagaagtc gtaccagccc
      841 gccgagcctg ctccgccaac accaagttgc ccggcctcgg cagcgatgcc ggtcgagctg
      901 ggggccaggg cacccgccgt ggcgcccatg gtgccgccaa ggcccccgtt ccctgctgcg
      961 gattctgtgg ccgacgcgtc gctgccgctg ccgccggctc ccgcgccggt gaccgcgctg
     1021 aagcctgcgc tgcctgcagt gccgccgctg ccctcgccgg agccggcgcc accgccgccg
     1081 ccggcgacca cgagccgcga atcggtgtcg gtcagcgcga cactgatcgg ctgggtacgc
     1141 acgtcggagg cgccgccgcc tgagccgccc caagatatga tgctcgcgcc tccgccgttg
     1201 aagcctcccg gggcgccgct tcggtcggac gcgggaacgc tacccggtcc gccgaccacg
     1261 acgtagagca cggtcgtgcc cagcggcaag ggcacggtgg ccgtcaccac cgcacgtccg
     1321 ccgccaggct tcgcgttgcg ccaatcggcc ccgccgacgg cgcctgcggc tgtgatcgtc
     1381 acagcggtcg tcccggccgg caccagatag atctgctcgg ttcccgtgta ccagtatgtc
     1441 gtcgccccag cctcagctcg ggcgacaacc tgtggagtcc ggtcagccgc cagagcgggc
     1501 gcggcgatga ccagtacggc gaaagcgacg aaaacgagcg cgagtttcct cttcctgctc
     1561 atgactcccc ctgtcccctt gcggctctgt ctgcgagacc gtaggagatg gccgatttcg
     1621 cgacatcacc cgaaaagggt ggcattcgcg ccgtggcgcg tctcaggagt ctcgcatgcg
     1681 gtcggtctac gcccgggcgg cggggagtgc aagcgtttgg aactggaagc aacactctcc
     1741 caactgagct agaggcccac gaggggactc cgtagaccgc gcgtagtagg gggaggaccg
     1801 gcttgagccc ggcgaccgtc ggccggacgc ggcctcgtca gcgcacgcgc agcgtgttga
     1861 ccgccggccg cgtctcgcgg ttcccgccgg agtcacgcgc gtgcacgctg tagcggtacc
     1921 ggccgcgcgg gaggtcgcac aggaagcgac cgtggagcca ggtgttcacc ctgcggcgcg
     1981 ggagcaccac cgtcttgatg agcctgcctc cgcgcgtcct caccttgatg gtgacgtcgg
     2041 ccctggggcc gaacggacgc ggatcgttga cgcgcatgcg cacgtccgcc atctcgtcct
     2101 ggtccacgat ctcggcgtcg ggagcggccg gcgtgggtcg ctgcgtgtcg ttccacacga
     2161 cctcgtgcag gtcgatcgtc tcgccggcgg ccagcgtgaa ggccttggcc tccgagcgca
     2221 acgcggtgtc ggggtagtag cgcttgtggc tcgggcggtc ggtgtcccag aaggcgacga
     2281 tgtaggtgcc ggccgggagg tggaagatcc ggaaggaccc gtcttcgtgg caacactccc
     2341 cggaccagtc gacccagcga ccggtgtcgc tgagccggaa ggcctgcgcg gccacgtcgc
     2401 gcgcgggcag gccgaagcgc gtggtgatgg tgccgtcgat gtgcgccgac gtgagcatct
     2461 tcacggcgac cgtcgtggtc tggccggagg tgacggagag cggcgtcgcg tcctcgaaca
     2521 cgcgctggcc gtcccaccac tggtagacgt agtcctcgtt ctcggtgtct tcgaaataca
     2581 gcttccaggc ggcgccaggc aggaggccgc cgatgtcgaa gccgccgtcg gcggccgtcc
     2641 tccccgtgcc ggcctgcccg tccatcccga cccagatact gatgccgccg agaggagtcc
     2701 cgtactcgtc ggtgaccacc ccggccacgc ggccggcggg cagcaactcg acgtcggccg
     2761 cgtaggcggt gtcgttggcg agcacgatct tcgtcgcctc ggccctcgtg aaggcgtcgc
     2821 tccagcagca ggacgcatag gccgaggccg ggtcgtggag ctcgaggacg taggtgccgg
     2881 cggcgagccc gtcgaagcgc acgcctcccg agaccgggtc gctgtacccg caccgccagt
     2941 cgccgcccca gccccacggc atggactggt acaggtccgc ccggatgccc gtgaggggaa
     3001 gcagcgtgtc cgcggctcgc acctggactg agatgctcgc ggcacgcgcc ggctgcgcag
     3061 cggccgccag cgccgccggc gcacagcacg cgaggaagag caggaacagc agacccgtcc
     3121 ccctgcgaac gcccatgccg acccccgacg ccgccggcgc gccctccacg cgccgcacta
     3181 ccttcatcat acacccggga ggcggcgctc tccgatggtg gacgccgaag agaagtactt
     3241 cgacggcgcc cggacgtacc ggctgacgct gcccagggac atccccgccg cccggttctg
     3301 gtccctcacg gtctacgccg gccagacccg ctccatgcac gcgacgcccc agggctaccc
     3361 gcgcgccggc agccggaggc gtggcactcg gcaactggat ccagaccctg cccggcagga
     3421 gctggtacgt gatgctgcgc ctctacagcc cgctcgagcc gtacttcgac aagtcgtggc
     3481 gggtgggggg gtcgagctgg tggggtgagg agcgggcctg gtgcagccgc ctcaccccac
     3541 gggctgcggc ttggggaacc gatagctccc gtcggcgatg gcctcgcgtg tcccgtacag
     3601 ccggacgagg tagcaccagc cctcgggcgt gtagatgaag ttgggctggt ccggatcgcc
     3661 gccgaagtgg atcgtgatgc tgccgtcgtc gttcggcttc gccgtgacgc tgttgacgat
     3721 gtaggcgtcg tagtcgttct tgttgaggct caggttgcgg tcgtagaccg tgaccgacca
     3781 gaacccgtcg acggggacat cgcgcacggt gagcgtgtgt ggcgtcgtgc cgtcgttccg
     3841 gggcggggaa ccctggagga tcagcgcctc gtgcggctcg ggcaccccgg tccaccccga
     3901 ggcgctggcg acgtagaact tcaccgggtc gacctcgccg acgtcgccga acgcgttgga
     3961 ccagtcgtcc atcgtgtagt agagcaccct gaacgcccca tcgatccgcc ggtatgactc
     4021 ccagtcccag tcggggatct cgaacgaacc ggcagacgcc tgctccacca cgatcgcgtc
     4081 ctggatcgcc tggacttctc tcacatcggc cgggtcgttc gggtccacga aggtgcggaa
     4141 gttcacgccg atgtagcgcg tgcctgcctg ctcccgcgtg atcgtgtacc tgccgggccc
     4201 ggtggtgctc agcttgaggt actggtcttc gttctggacg agcatcgact ggtaccgtcc
     4261 gctggtctcg ggcttggtga tcgtcaccgg ctccgtcagg tcgaagacgc ccagcgagta
     4321 gagggtgtcc cggttctccc cgatgcccag ctggccctcg tcgacgggcg cgagatccct
     4381 cacgtggtag aacctgccga ccccgaccct gcccacgttg gcccggaacg aggcgtggca
     4441 gttggcccgc gggtagttct cccacgtgac ggagaccgtc ccgccggctt ctgcgttgga
     4501 ttgtgtcccg tcgctcatcc ccgatgctcc cttcagccgc tcctgggtgc tccgcatgca
     4561 gccccagcct gcttgaatcc tcgggtaggc cgaggcggca cacaatgtct cgagggacag
     4621 tcgggggaca gtgtggcggg acagtctgac tcaggccacc ggctccacct cgccgccctc
     4681 cgtcacgcct cgacgagctc gatctcgccg ggccgccagg tcttgtcgaa ccagggctcg
     4741 agcggcccgt agaggcgaag caccaggaac cagcccttgc cgggcacggt ctggagccag
     4801 ttcgcctcgt tgccggcggg cggctccgga ccgaagtaca cgtccactga ggtgtcggcg
     4861 ttcgcctgga ggcccggccg ctggctgccg accatcggga agcgctggtc ggtctgcagc
     4921 atcgaccgcg tctgcgggtc gtacacggtg agggaccaga agtccttgac ggggatgtcc
     4981 ggcggcaggt ggagtgtgta cgtcctgccg ccgtccaggt accggccctc ggcatcgtgg
     5041 tcggcccagg cgtactgcga ggccttcccc accatcttca cccacatcgc cgggctgatc
     5101 tggtccgtga tgctgaagta gaacgtgcgc gcgtcgaggt cgagcacccc gcccggcgag
     5161 aagtcggtgc cgtcgccctt gtaccccatc ttccacgcgc cgtccggata gtagaggagc
     5221 tccgggtccc ggggactgaa gaggacggca cgcgccgtgg cgttgccgac cgcggcggcc
     5281 tcggcgagga tcgcctgcat ccgcgcgtcg ggcgcgaacg gcgcgtcctt gcggatgccg
     5341 acggccgcga gcaggccgcg cgtctccggg tcgacggcct cgagcggctc ctcctggacg
     5401 acggtggcga cggactcgaa gagcgacgcg tcgctgggcg ggatcgcgtt catgtacttg
     5461 ccggagatgt ctacgaagtc ggtcgccggc cggtcct
//
LOCUS       DASUMX010000184        25429 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_353655, whole genome shotgun sequence.
ACCESSION   DASUMX010000184 DASUMX010000000
VERSION     DASUMX010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 25429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25429
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353655"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VFZ86_09115"
     CDS             <1..258
                     /locus_tag="VFZ86_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018158159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX5642481.1"
                     /translation="EVWRAGREIELTATEFKLLRYLLLNARRVLSKDQILDHVWNYDF
                     GGNANIVETYVSYLRRKVDAEGAPLIHTVRGVGYSLRLPKD"
     gene            347..1753
                     /locus_tag="VFZ86_09120"
     CDS             347..1753
                     /locus_tag="VFZ86_09120"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5642482.1"
                     /translation="MSLRTRLLVAVLGCVAAGLVVAGLITYFSLRGFLLERVDAQLRD
                     ARVPTAMALAGSQGGLPGGPGEEANLPPGTYGQLRSTGGDVLNAIQFSYSLEELPTPV
                     LPDPLPVATDRRGTVFTAGSTAGGQAPGFRVLVENVPDLGRVLVVAIPLNETRQTLTR
                     LLTIEVVVSLAVLAALAAAAWWLIKRDLRPLETMAVTADAIAAGDLARRVQPAEPRTE
                     VGRLGLSLNTMLGRIEEAFAERAATEEKLRRFLADASHELRTPLTSIRGYSEVFDRAK
                     DDPADLELAMRRIEEESKRMGVMVEELLVLARLGEGREPERLPVDLARVVADAVSDAR
                     AAKPERDIALESPEAVDVLGDDHQLRQVVANLLDNALRHTPEEARVQVALRAEDGRAE
                     LAVADDGPGLAADVAAKVFEPFYRADTSRARETGGAGLGLAIVAAIVEAHGGGVRLDT
                     APGAGATFTVTLPLLGGR"
     gene            complement(1750..5046)
                     /locus_tag="VFZ86_09125"
     CDS             complement(1750..5046)
                     /locus_tag="VFZ86_09125"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5642483.1"
                     /translation="MVTTLFCDLVAFTAMSDEADPEDVDATLRHYHAAVCRVIEAHGG
                     VVEKYIGDAVVGVFGVPSVHEDDAERAVRACLRIVQALTGMTRPDGSALEARCGVDTG
                     EAVVRLDVDPASGCGFLVGDAVNTAARLQAAAPPMGVAVGARSYELTRHVFVYEELSP
                     VAAKGKKAPLAVWRPVRPVARTGLRTSIHPGGPFVGRHDELHALEETLAAVRALGRRR
                     SCLIVGEPGIGKSRLVLEFARTLESDPESLVWRQGRCLPYGEGVALAPLAEIFRAHCG
                     ILDSDDPRAMDRRIEAALPPSADAAWLRQRLRALLGLPAPASPRDENFSGWRRILDGL
                     ATSHPAVVVFEDLHWGHDVLLDFIDSFVVESGSKPLLLVATTRPELLARRPSLAAPGS
                     GWSRSLLQPLTPADIERLVARSQRGPSHERAAAIAELSGGNPLFAETYAGLLADGGVL
                     PASGPTGVAGGQSPPAPDAIRAVLAARLDTLPRAHKTALADASVFGEAFWLGGVEAMS
                     PLPGAELARIFAALVDRQFVRPAATSSLQGEEEYLFWHALARDVAYGQQTRAERARRH
                     AAAAAWLEEKGRERPEDHAGVVAHHYATAHDLAKAAGRLELAEALRPLAARSLEFAGT
                     HALAVDVPAAERYLARALELIDRADAMYGRALAGRARALLRTGRNHEAESLIDGAVAS
                     LRAAGDLRGTASALCVQSDVAAELGKPYVHFVEEAGLLLADDGPSPELTRVLSRLSWV
                     PLQQRSAGGPEEAIRRSERTIAACGDLDVPEAVDALDCRGMARIDLGDVGGLDDLQDA
                     LAAASRLGLGEELLVLQFNTGSALLALRGVEASMAMSRSGMEAARLRGSSSFLRAHRA
                     GLFVGLRHAGAWNEALAEARDLEPELDRADDVFTHVLVRSVLAVLLVERGQAGEASPW
                     VEWLIEKGRENEIRWCKVLALAGSAVALQALGQGQRAMKLLREVGALERGVVAAIGQE
                     IVLPVLRDVLHEGDVGLALRLCSIERLGTPWDHHVDTTVRAALAEARGDHEAAAAGFS
                     DAAGRWREWGVPAEQAHALFGLARCLTMLGRAREAAGPLAEARDVFARLEARPALAEA
                     DAWSARGTGV"
     gene            5386..5640
                     /locus_tag="VFZ86_09130"
     CDS             5386..5640
                     /locus_tag="VFZ86_09130"
                     /inference="COORDINATES: protein motif:HMM:NF018058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HEX5642484.1"
                     /translation="MTQSSGSQKSGGNGFLIGGGVLLALLLIFIFQNTAETTFKFLFW
                     SFTFPLWLGLLITAIVAFVVGQFALMWRRHKRRQARRDAR"
     gene            complement(5723..6046)
                     /locus_tag="VFZ86_09135"
     CDS             complement(5723..6046)
                     /locus_tag="VFZ86_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642485.1"
                     /translation="MEVDAPLEFADLEWSEFLMDSLLHGYARGLIDAEPLLDEDDMEA
                     GKVEFITEGERLVRVEVRLDYTPRSKSAADQELRRAQATLERDLEKYRLFVRDRCDRL
                     QCRPE"
     gene            6264..7766
                     /locus_tag="VFZ86_09140"
     CDS             6264..7766
                     /locus_tag="VFZ86_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HEX5642486.1"
                     /translation="MNTAPPLTITPKTTIHTLLKEYPFLLDFLAEYHPEFKKLTNPVL
                     RRTVGRMATLDAVAEQGNVPLNRLTDDIATEVEKRTGTRPPVADVANAETIDPARLAE
                     LHAIVKDLHAGKSVEEVKPRFEELIQDVEATEIAAMEQKLIEGGVPDTEVKRLCDVHV
                     QVFADALEGHAAVTAPPGHPLDTFQRENQALLQVTASLRKVAEAIGEPPDGAAWARLK
                     GALSDTVERLAEVDKHYLRKENQLFPFLEDHGVEGPSKVMWAIHDDIRALVKQARATI
                     TEDDAAMAVSTCLALAKMVDDMVTKEEKVLHPMAIDTLSEAEWAKIRAGEDDIGYAFI
                     EDVPAWPAAGTAAEAGAAGAPAGAAEAGALAAAGLLALDTGELNLEELNLVLGVLPID
                     FQYVDEHDRVRFYSEGHRIFPRSPGVIGRKVQNCHPPASVHKVQQIIDAFRAGEKDTA
                     DFWIEMQGKFLHIRYFAVRDGGGAYRGVVETVQDVTAIRALEGQRRLLDW"
     gene            7840..8232
                     /locus_tag="VFZ86_09145"
     CDS             7840..8232
                     /locus_tag="VFZ86_09145"
                     /inference="COORDINATES: protein motif:HMM:NF024983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX5642487.1"
                     /translation="MLWKHGGLTPELADLVFTFLPLDLSVADENDVLVYWKGDTYKTC
                     DARYIGRDVRDCHPEHSMACLEEILSAFKAGTKDVAEGWEQKGETMKRTRYIALRDHD
                     GAYKGILEVNEDVTSARALEGDQALPGW"
     gene            8260..8385
                     /locus_tag="VFZ86_09150"
     CDS             8260..8385
                     /locus_tag="VFZ86_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642488.1"
                     /translation="MTTLVAATGAGAWLLLIGAAIAGLAFGMIMNSVLTGRRKKK"
     gene            complement(8396..10126)
                     /locus_tag="VFZ86_09155"
     CDS             complement(8396..10126)
                     /locus_tag="VFZ86_09155"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX5642489.1"
                     /translation="MRPRWRAGVLAAAGAATLLAGCGIRVAAGPSPSTAPARTATLVA
                     SAPASPSAAPSPQVAPPQDVPSAVPTAAPSAAPAPALSGRRAAKLAAAARRVSAAAAV
                     RRALPALDPLVASMVARSGVPGAAVAVVAGDTVMYSRSFGLREMGRPDPVDDDTLFQL
                     GAVSRAYTSTMLAALAGEGELGWDQPVRRAWPGFRLRDPWATREATFRDLTAGRSGMP
                     AHAGDELLAFGYGRAEVLRRLRQLRPAAGFRAEFAPQDGMVTAAAAAAERATGRSWAR
                     LVRERVLEPVGDHGTVLTWRGFLRAVDKATPHRLVAGTMVPQDPADESVFAPSLGVSA
                     SLSGLVTFARLQLNGGAVAGARVAPAGLLAQTLRPATAVADTPSGPEAAAPGWMLSTF
                     DGRLLASAEGGLASGSSATVTLLPDDGVAVIVLANAYPEGAALGGAIARTLTDLAVLG
                     APQEDWLAREQAALATERASVPAAGLALPPQAPAAAPAPRARDVYAGVYENRYYGRVT
                     VRPDAGDGLVVRLGRGQTLRYAPWSADIWRDMASGTAAVFAVRGGRAQAVTLTQLTFD
                     GRRGAFARVD"
     gene            10306..11643
                     /locus_tag="VFZ86_09160"
     CDS             10306..11643
                     /locus_tag="VFZ86_09160"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX5642490.1"
                     /translation="MTPHVEDRERTDIATCLGEIDRALTDVLEGRGVHYVAEATCSPV
                     ALPLVHTVNRLLRQLEEIVRFVLPLSRGELSAPLPPPDNRMAAALVQMHARLRQLTRQ
                     TQEIARGDYTQRIDFMGEFSEAFNAMVALLEERERTLTDEIARRRQAEDELQRERDLL
                     VAGPVVTFRWGVDDDGTVEYVSPNVSAYGYAPEEFTSGRRVYAGIVDPADIGWISEDG
                     NDKSRAGLDCWTQEYRIIDAEGQTHWVRDFTHTVRDADGAATAYEGYIIDITPQKAAE
                     TALRQREEQLRMLSLADGLTGLYNRRGLFALGEHAMRSARRRAGGLGVVYVDVDGLKA
                     INDRFGHAQGDQALCAVADVIRASIRESDVVGRVGGDEFVVLAEDDPSVTRELVARLR
                     RRMDRANERSGRPFRISLGIGAADWEPGEQVTLQELIERAGQRMHDDRRERRR"
     gene            11640..13217
                     /locus_tag="VFZ86_09165"
     CDS             11640..13217
                     /locus_tag="VFZ86_09165"
                     /EC_number="1.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009376823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinic semialdehyde dehydrogenase"
                     /protein_id="HEX5642491.1"
                     /translation="MSPGHSQHQWSPRVSAAMLARLAARAAVAAPAGATPVEAPFTGA
                     VLATVPKGRPADVEAACAAAREAQVEWARRPIEERARVFLRYHDLVIACAQGLLDLIQ
                     LESGKARIHAYEEVLDVAIQARYYAHAAERYLREQRKQGALPLLTSTRVYHRPRGVIG
                     IVTPWNYPFTLAISDALPALVAGNGVVLKPDTQTPLTALRAADLLEEAGLPRGLLQVV
                     TGRGSELGEPLVDAVDYVMFTGSTATGRTVAAQCGARLKDCSMELGGKNALLVLPDAA
                     LWRAVPGALQGITSSSGQLCISIERLYVHDAVYDVFVPRLAERLKAVRLGSSLTFADD
                     MGSLASADQLAKVAAQVDDAVGKGAQVLAGGSARPDLGPYFYEPTLLAGVTEDMELCR
                     AETFGPVAAVYRCSSVDEMVARANDSEFGLNASVWTRDVEAGRAIAARLQTGTVNVNE
                     AYAATWASAAPMGGWKQSGLERRHGRHGILKYTDPQTVSVQRLLNIGTPPLLSHGQYA
                     TVMTRALRLLKHVPGIK"
     gene            complement(13266..13970)
                     /locus_tag="VFZ86_09170"
     CDS             complement(13266..13970)
                     /locus_tag="VFZ86_09170"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5642492.1"
                     /translation="METSYDKDGLYLGRLADVFGDPTRRGIYRHLRGCEQPQSASEVA
                     EVFGLHRTVARAHLERLTELDLVTTATRRRAGGGRPAKIYAVSDARLEIMLPPRRYER
                     LARLLLRLVHETLEPEVAATTAAALGRTCGEQTAEEFAGGDTRAPAALSPRAVMDWMD
                     LAGYGATLSANGNGATVIEVHNCVYRELSQEYPDVVCAFDRGMLCGMLGVDASVHAQT
                     RALSRGDAYCRHEFQL"
     gene            14175..14378
                     /locus_tag="VFZ86_09175"
     CDS             14175..14378
                     /locus_tag="VFZ86_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642493.1"
                     /translation="MIKVRSFATPIKIFATMRELHDLDAQVADFLNEEGADKVFSMSD
                     STTVGEHGETIGLIRAVAYRVPD"
     gene            14414..15316
                     /locus_tag="VFZ86_09180"
     CDS             14414..15316
                     /locus_tag="VFZ86_09180"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010491076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5642494.1"
                     /translation="MSHDEDTAGEGPAHPIPPEARAANLALWNHWTTVHEKSAFYDVE
                     GFKAGATSLWPIEREELGPLVHEGTSLLHLQCHFGLDTLSWARLGAGVVGLDLSDEAI
                     GLARRLAAEIGVADRAEFVCADLYDADAHLGGRLFDIVFVSWGAIEWLPDLERWAGIV
                     ARHLRPGGTFYMAEIHPFAYALDEVPGEHDVQVKYRLLPAPEAPDVDPVEGSYADPGA
                     DTTGLTAYGWTHSLAEITGALIGAGLRLEHLHEFPTSPSPFWSWMVRDEDRWWWLPDA
                     EGGLRKDLPLSYSLRASKPANAEG"
     gene            15319..16419
                     /locus_tag="VFZ86_09185"
     CDS             15319..16419
                     /locus_tag="VFZ86_09185"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5642495.1"
                     /translation="MLDDYLKANLASWDEAVGLHVGSELYDVAGFKAGRTALSAIELD
                     ELGPLVGEGTRLLHLQCHFGLDTLSWARRGAEVTGVDFSGEGIRTARVLADEVGLASR
                     ATFVQSDIESLPDKLTGQFDVVFTSWGALIWLADLERWADVIAHFLRPGGVFYIAEFH
                     PYAFLLADDATPDSLRVGYPYFMHGVPQRFDEDGDYADPDAKVKNTVTFEWLHGFAEI
                     VDPLLRRGLRLDFLHELPYTVPGLQLPFVEKDDRGMQRVKGHHEDFPLSFTLKMTREG
                     RTSGRAGRPPGDRARLLGDEDLLAILEEAIADERAAQEKYRRGLERCADPAACEVFEQ
                     LLREEQAHERVLEARYVEVKKRVGLSGAGRTD"
     gene            complement(16632..17462)
                     /locus_tag="VFZ86_09190"
     CDS             complement(16632..17462)
                     /locus_tag="VFZ86_09190"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HEX5642496.1"
                     /translation="MLPPAFRPALVAADLDGTLIARDLSWAEGLPEAVADLAPAGIHA
                     VVCTGRMFQSARRVAARLGVSAGPVVCYQGALVADLGTGEWLRHVPMDGRAAAEVVRH
                     VAGMGRQLNAYIDDNLYVEQVTEWARRYAEHVEVGIHTVADLEAEVVRRPPTKLVLVT
                     SPADADAVLAGLQERWRGRLYVVRSQPEYIEFADASVSKSGALAWLCARLGVAREQVV
                     TLGDGMNDVDMLAWAGLGVAVAEADEPVRAAADLVVPRAGIPGLLRELARGADPRAQK
                     "
     gene            complement(17631..18185)
                     /locus_tag="VFZ86_09195"
     CDS             complement(17631..18185)
                     /locus_tag="VFZ86_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642497.1"
                     /translation="MTPLARVIAVIVGIVTLLLAGGALVRQAAMAVDAGVSWPIAAWW
                     ADLTIRSSTATIVAAAVMTVVTVLLIVFAYRQVSPGATPQVIEFSVPEGTARLSVPAL
                     RKALRRRLEAMLPGSRVTEIAVRMDDAGWSVRVEADVPVCDLRDVHGAVLEAFRDDMR
                     RVAAVDLVRLDLIVTSMRAPARKA"
     gene            complement(18182..18637)
                     /locus_tag="VFZ86_09200"
     CDS             complement(18182..18637)
                     /locus_tag="VFZ86_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642498.1"
                     /translation="MALDWDFSPFAYITPNGHALLAWLLLLAMVVLATLVWLVLRHDS
                     DSLWLASPAGDGGVLVPNEALERPAASASGRSHPDVVRADVELFKRGADLRGRVTVWA
                     RPLADAGLVGGAVDAAVRRQVARLTGRELERLDVRVKVLKVTQLVRYLP"
     gene            complement(18750..19133)
                     /locus_tag="VFZ86_09205"
     CDS             complement(18750..19133)
                     /locus_tag="VFZ86_09205"
                     /inference="COORDINATES: protein motif:HMM:NF015721.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEX5642499.1"
                     /translation="MSETPKPTAAPGGGGVTISDSVVAKVAFKAIGGIDGIHALGGTS
                     ARALAGLRGDKGTPGISVDLREGSVDIDITMSIVFGASVPAVAEACRKAVSEQVEATT
                     GLPVRAVNVLVTDVVFPEDQAGAAG"
     gene            complement(19241..21493)
                     /locus_tag="VFZ86_09210"
     CDS             complement(19241..21493)
                     /locus_tag="VFZ86_09210"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HEX5642500.1"
                     /translation="MTETGKAVIELSANALKVLQRRYLKKGENGELLETPEEMFRRVA
                     RAVAQAERLYNPAAPVEEWEGTFYELMTSLEFLPNSPTLMNAGRELGQLSACFVLPVG
                     DSMESIFEAIKNTALIHKSGGGTGFSFSRVRPHNDVVNSTRGVSSGPLSFMSVFDAAT
                     ETIKQGGTRRGANMGIMRVDHPDVLDFITAKQDDDRLNNFNISVAVTDAFMRAVEADE
                     EYALVNPRSGETVRTLSARKVFKNMVNLAWKNGDPGIVFIDRINEDNPTPQLGDIEST
                     NPCGEQPLLPYESCNLGSINLSRVVPDGHIDYGALGRIVRTAVRFLDDVIDVNRYPLP
                     EIEQMTKGNRKIGLGVMGFADMLLRLQVPYDSDEACRIAGEVMGFIHDEARAESEDLA
                     RERGVFPNFEGSIYDSPAGARVRNATVTTIAPTGTISIIAGCSSGIEPIFALSYVRAN
                     VLDDDRMVEGHPYFEQVARERGFYSDELMKRIAEVGSARHTDGVPQDVQQVFATAHDI
                     TPEWHIKLQAAFQKSTDNAVSKTVNFPNEATVEDVENVYRMAFRTGCKGVTIYRDGSR
                     DEQVLNVGQTKKKREPEPGLGIATTRPARPQLLTGETQRMDTGCGKLFVIMNDDEYGP
                     REVFANMGKAGGCASSNTEALGRLISLALKKGASAQEIVEQLKGIRCHVPYGMGPNAT
                     LSCADAIGKALERRYVAGAAARASATPEPQLSLVEVAQGACPECGSTMRHEGGCAVCN
                     DPSCSYSKCS"
     gene            complement(21486..22064)
                     /gene="nrdR"
                     /locus_tag="VFZ86_09215"
     CDS             complement(21486..22064)
                     /gene="nrdR"
                     /locus_tag="VFZ86_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013376883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEX5642501.1"
                     /translation="MRCPFCSSDESKVVDSRDSEAGDAIRRRRECLACERRYTTYERI
                     EEVPLVVIKSDGREELFTRHKVLNGLLRACEKRGIALDRLERACDDIENDLRRVPGRP
                     VTTRMVGERALRHLRDIDKVAYIRFASVYRQFEDIEEFQQELAQLELAGVEPLPGEEP
                     LPGIDAGESFIDEIIFTTPRRAVTTEGRTGHD"
     gene            22303..23034
                     /locus_tag="VFZ86_09220"
     CDS             22303..23034
                     /locus_tag="VFZ86_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014395501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HEX5642502.1"
                     /translation="MNVIVLPTYNERDNLRHALGRIREVADGHGVQLHTLIVDDNSPD
                     GTGALADELAREVPDVSVMHRAGKEGLGKAYIAGFREALAMGAERIFEMDADLSHDAT
                     YLPHFLRLIDQGADLVLGSRYVRGGGVENWGLSRKIISRGGCLYAQTILGLPLRDLTG
                     GYKCFRRAVLETVDLDTVDTKGYGFQIEMTYRVYKLGFKVVELPIIFVDRKVGESKMS
                     NDIVVEAMKNVWKLRFSRVPTRRTA"
     gene            23059..24177
                     /locus_tag="VFZ86_09225"
     CDS             23059..24177
                     /locus_tag="VFZ86_09225"
                     /inference="COORDINATES: protein motif:HMM:NF020318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1786 family protein"
                     /protein_id="HEX5642503.1"
                     /translation="MTSPAQPAGVLAIDVGAGTTDILFTRPGQPLENAVKLVVPSATQ
                     VVGARIAAATARGQTVVFRGPVMGGGAVTAAMKAHLAAGQAFVATETAAASFADDLDR
                     VRALGARVVGEGAADEVLRRLPAGAAVEVASGDLDAAALTQALRLLGAEPAFAAVAVA
                     VQDHGYAPGGSNRVLRFALWQQAVAERRPIGELFYAPVAVPPSLTRLCAAADAATALA
                     GGAPALVADTGPAALYGALPREVENAVLVNVGNGHTVCLLALEGRVAGVFEHHTSAID
                     GPGLELRLRRWLAGDLESEEVRADHGHGAVLAPGAADGDPLRLPLVVTGPRRELLAGS
                     QLPVEFAAPHGDMMLTGCFGLLRALCERHAGLREEPAA"
     gene            24174..24692
                     /locus_tag="VFZ86_09230"
     CDS             24174..24692
                     /locus_tag="VFZ86_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HEX5642504.1"
                     /translation="MSRPLSTGLVVPVDAARQLVAPWLDRLPAAGRALPPHVTALWPF
                     LPAVAADDAVERRLQELLAGARAFPFTLERVAGLADAVVLVAEPAEPFTALTRLLWDA
                     WPECPPFGGAYDDIAPHVVVAIDPSTADRAAIEATLAPRLPLAARAAEVLLVEEVESG
                     AVRVRRRFSLGA"
     gene            complement(24689..>25429)
                     /locus_tag="VFZ86_09235"
     CDS             complement(24689..>25429)
                     /locus_tag="VFZ86_09235"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642505.1"
                     /translation="RGLTITNGLVVLSDPVTGLPAAVMDAATVTAWRTGASVGVAARH
                     LARPDAATVGVLGCGVQARASVRALATVLPELREVRCHDATTAAGEAFAVALGAELPD
                     LRFTRVAAPEDVVRGAGVVVSAITMTADIRPPLGPGLLEPGALAVALDYDAAWSAPAM
                     SECDRFYCDDTATVWATQAAGVRLAGIPGDVAGDLGELAAGLVDGRRTPDERLFCLNL
                     GMALEDVVTGELALRRARELGAGRELPL"
BASE COUNT         3740 a         9309 c         8969 g         3411 t
ORIGIN      
        1 gaggtctggc gcgcgggccg cgagatcgag ctcacggcca ccgagttcaa gctgctccgc
       61 tacctgctcc tcaacgcgcg ccgcgtgctc agcaaggacc agatccttga ccacgtgtgg
      121 aactacgact tcggcggcaa cgccaacatc gtcgagacct acgtcagcta tttgcggcgc
      181 aaggtggacg ccgagggcgc gccgctcatc cacaccgtgc gcggcgtcgg ctacagcctg
      241 cgcctgccca aggactaatc ggcgaagatg gacgagatca acggagctct attgagcggc
      301 cgccggcccg cagggaccca gcggcggacg gactgaaggc gacaccgtga gcctgcgcac
      361 ccgtctgctg gtggccgtcc tgggctgcgt cgcggccggc ctggtcgtgg ccggcctcat
      421 cacgtacttc tcgctgcgcg ggttcctgct ggagcgcgtc gacgcgcagc tcagggacgc
      481 gcgcgtgccc accgccatgg cgctggcggg cagccagggt ggcctgccgg gcgggccggg
      541 cgaagaggcc aatctgccgc ccggcacgta cggacaactc cgcagcaccg gcggcgacgt
      601 cctcaacgcc atccagttca gctacagcct ggaggaactg cccacgcccg tcctgcccga
      661 ccccctgccg gtcgcgaccg accgccgggg tacagtgttc acggccggat ccacagccgg
      721 agggcaggcg cccgggttcc gggtgctcgt ggagaacgtc cccgacctcg ggcgcgtgct
      781 cgtcgtggcc atccctctca acgagacccg gcagacgctg acgagactgc tgaccatcga
      841 ggtcgtcgtc tcgctcgcgg tgctcgccgc gctggcggcc gccgcctggt ggctgatcaa
      901 gcgcgacctg cggccgctcg agaccatggc cgtcacggcg gacgccatcg ccgccgggga
      961 cctcgcgcga cgcgtgcagc cggccgagcc gcggacggag gtcggacggc tcggcctcag
     1021 cctgaacacg atgctcggcc gcatcgagga ggcgttcgcg gagcgcgccg ccaccgagga
     1081 gaagctgcgg cgcttcctcg ccgatgcctc gcacgagctg cgcaccccgc tgaccagcat
     1141 ccgcggctac tccgaggtgt tcgaccgcgc caaggacgac cccgcggacc tcgagctggc
     1201 gatgcgccgc atcgaagagg agagcaagcg catgggcgtg atggtcgaag agctgctggt
     1261 gctcgcgcgg ctcggcgagg ggcgcgagcc ggagaggctg ccggtggacc tcgcgcgcgt
     1321 cgtggccgac gccgtgagcg acgcgcgcgc cgccaagccg gagcgcgaca tcgccctcga
     1381 gagcccggag gccgtcgacg tcctcggcga cgaccaccag ctccgccagg tcgtcgccaa
     1441 cctgctggac aacgcgctgc gccacacgcc ggaagaggcg cgagtccagg tggcgctgcg
     1501 ggccgaagac ggccgcgccg agctggccgt cgccgacgac ggccccgggc tcgcggcgga
     1561 cgtcgccgcc aaggtgttcg agcccttcta ccgcgccgac acctcgcgtg cccgcgagac
     1621 cggcggcgcc ggcctcggcc tcgcgatcgt cgccgccatc gtggaggccc acggcggcgg
     1681 cgtccggctc gacaccgcgc cgggcgccgg cgcgaccttc acggtcacgc tgccgctgct
     1741 cggcggacgt tagacccctg tgccccgcgc cgaccaggcg tccgcctccg ccagcgcggg
     1801 cctggcctcg agccgtgcga agacatctct ggcttcggcg agcggccctg ccgcctcccg
     1861 cgctctcccg agcatggtga ggcagcgcgc aaggccgaac agcgcgtgcg cctgctccgc
     1921 tgggacgccc cactcacgcc agcgaccggc ggcgtcggag aagccggcgg cggcggcctc
     1981 gtggtcgcca cgcgcttccg ccagcgccgc ccgcaccgtc gtgtcgacat gatgatccca
     2041 gggagtgcct aaccgctcga tggagcacag gcgcagtgcg agcccgacgt cgccctcgtg
     2101 aagcacgtcc cgcagcaccg gcaggacgat ctcctgcccg atcgctgcga ccacgccgcg
     2161 ctccagcgcg ccgacctccc gcaggagttt catggccctc tgcccctggc cgagcgcctg
     2221 gagcgcgacg gcgctgccgg ccagggcgag caccttgcac cacctgatct cgttctcgcg
     2281 acccttctcg atcagccact ccacccacgg cgacgcctct ccggcttgac cgcgctcgac
     2341 cagaaggacg gccaggacgg atcgcaccag cacgtgcgtg aagacgtcgt ccgcccggtc
     2401 gagctccggt tcgaggtcgc gagcctccgc gagggcctcg ttccacgcgc cggcgtggcg
     2461 aagcccaacg aagagtccgg cgcggtgagc gcgcagaaag cttgagctcc cacgaagccg
     2521 ggccgcctcc atcccgctcc tggacattgc catcgaggcc tcgacgccgc gcagagcaag
     2581 gagcgcggaa ccagtgttga actgcagaac gagaagttcc tcaccgagac cgagccggct
     2641 cgcggccgca agggcgtctt gcaggtcgtc gagtccgccc acgtcgccaa ggtcgatgcg
     2701 cgccatccct cggcagtcca gggcgtccac ggcctccggg acatcaaggt ctccgcaggc
     2761 cgcgatggtc cgctctgacc ggcggatcgc ctcctcgggg cctccggccg agcgttgctg
     2821 cagcggcacc cacgagagcc tcgacaggac ccgggtcagc tcaggcgacg gcccatcatc
     2881 cgcaagcaga aggccggctt cctcgacgaa gtggacgtac ggcttgccga gctccgccgc
     2941 cacatcgctc tggacgcaga gagccgacgc tgtgcctcgg aggtctccgg cggcgcgcag
     3001 ggacgccacg gcgccgtcta tcagagactc ggcctcgtga ttccgtcccg tccggagcag
     3061 ggccctagcg cgacctgcga gcgcccgccc gtacatcgca tcagcccgat cgatgagctc
     3121 gagcgcccga gcgaggtagc gctcggcagc ggggacgtcg accgcgagcg catgcgtgcc
     3181 ggcgaactcc aggctgcggg cggcgagcgg ccggagcgct tcggcgagct cgaggcgacc
     3241 tgctgccttc gcaaggtcgt gcgcggtcgc gtagtggtgg gcgacgacgc ccgcgtgatc
     3301 ctcggggcgc tccctcccct tctcctccaa ccacgcggcg gcggcggcgt ggcggcgtgc
     3361 ccgctccgcc ctcgtctgct ggccgtacgc cacgtcgcgg gccagcgcgt gccagaagag
     3421 gtactcctcc tctccctgga gcgaggacgt cgcggctgga cgtacgaact ggcgatcgac
     3481 gagcgcagcg aagatcctcg ccagctctgc gcccggcagt ggagacatcg cctccacgcc
     3541 gcccagccag aaggcctcac cgaacacgga ggcgtcggcg agtgccgtct tgtgcgcgcg
     3601 cggcagcgta tccaggcgtg cggcaagcac ggcgcggatc gcgtccggcg cgggcgggct
     3661 ctgcccgccg gccactcccg tcgggccgga ggccggaagg acgccgccgt cggcgagcag
     3721 cccggcgtac gtctcggcga agagcgggtt tcccccggag agctcggcga tggcggcggc
     3781 acgttcatga gacggccccc gctgcgacct ggcgacgagt cgctcgatgt cggccggcgt
     3841 cagcggctgc agcaagctgc gcgaccagcc cgagccgggc gcggccaggg acggtcgcct
     3901 ggcgagcagt tcgggtctgg tggtcgccac gagcagcagc ggcttggagc cactctcaac
     3961 cacgaacgag tcgatgaagt ccagcagcac atcgtgcccc cagtgaaggt cctcgaagac
     4021 gaccacggcg ggatgtgaag tggcgaggcc atccaggatg cgtcgccacc ccgagaagtt
     4081 ctcgtcgcgg ggcgaagcgg gggcaggcag ccccaggagc gcgcgcaggc gctgccgcag
     4141 ccacgccgca tccgccgatg gcggcaacgc cgcctcgatc cgccgatcca ttgcccgcgg
     4201 atcgtcgctg tcgaggatcc cgcagtgcgc cctgaagatc tcggccagcg gagcgagtgc
     4261 gactccctcc ccgtagggaa ggcagcgccc ctgccgccat accagggact ccgggtcgct
     4321 ctccagcgtc cgcgcgaact ccagcaccag ccggctcttc ccgatgccgg gctcgccgac
     4381 gatcaggcag gagcgacgcc tgccgagggc tcggactgcg gcgagcgtct cctcaagcgc
     4441 atgcagttcg tcgtggcgcc cgacaaaggg cccgcctgga tggatcgacg tgcgcaggcc
     4501 ggtccgcgcg acgggcctga ccggccgcca caccgcaagg ggagccttct tgcctttggc
     4561 ggcaacaggt gacaactcct cgtacacgaa gacatgccgg gtgagctcgt aggagcgcgc
     4621 gccgactgcg acgcccatcg gcggcgccgc cgcctgcagt ctggcagcgg tgttgacggc
     4681 gtcccctacg aggaaaccac acccggacgc cggatcgacg tcgaggcgca cgacagcctc
     4741 tcccgtgtcc acgccgcacc gggcctcgag agcggaaccg tccgggcggg tcataccagt
     4801 aagcgcttga actatgcgca gacaggcgcg cacggcgcgc tccgcgtcgt cctcgtgcac
     4861 ggacggaacg ccgaacactc cgaccacggc gtcgccgatg tatttctcga ccacaccccc
     4921 gtgggcctcg atcacacgac acacggccgc gtgatagtgc cgtagcgtgg cgtccacgtc
     4981 ctcgggatcg gcctcatcac tcatcgccgt gaaagcgaca aggtcgcaga agagcgtcgt
     5041 gaccactttg cgctcttcgg tcgccccgtc cgccgccggc gcgagcggcg aaccacagct
     5101 ggggcatgcc cacgtcccct ccgcacaaat gagcccgcag tcaggacaag ggtccatagc
     5161 cccccccatc acgcgccgtc gcgatgcgag ctgcctcgac tccccttgca gtcgctcacc
     5221 cgattgtatg cccggacaga ccccacggcc gtttcggggt ccacgcgccg ggcaggaagc
     5281 agcggcatct gcggcgtacc ccacgggtaa gacggtcacg atgtatgccg cgcgactgca
     5341 ccgggagtcc gcgggggcaa gcgaggcact ccgaggaggc gcgacatgac acagagctca
     5401 ggctcacaga agtcgggcgg caacggcttc ctgatcggcg gcggcgtcct gctggcgctg
     5461 ctgctcatct tcatcttcca gaacaccgcc gagacgacgt tcaagttcct gttctggtcg
     5521 ttcacgtttc cgctctggct cgggctcctg atcaccgcca tcgtggcgtt cgtggtcggg
     5581 cagttcgcgc tgatgtggcg gcgccacaag cgccgccagg cgcggcgcga cgcccggtag
     5641 ccgctccagg agaccgttac ggcgggcggg gcgccggcgc ggccgcgaag cggctcctcc
     5701 ggcgccccgc cgcatcaccc ggtcactccg gacggcactg cagccggtcg cagcggtcgc
     5761 gcacgaacag gcggtacttc tccagatcgc gctcgagcgt ggcctgcgcg cggcgcagct
     5821 cctggtcggc cgcggacttg ctcctcggcg tgtagtccag gcgcacctcg accctcacca
     5881 gtcgctcacc ctcggtgatg aactcgacct taccggcctc catgtcgtcc tcgtcgagca
     5941 ggggttcggc gtcgatcagg cccctggcgt agccgtgcag cagagaatcc atgaggaact
     6001 cgctccactc gagatcggcg aactccagtg gtgcgtcgac ttccacggac gcttggatcg
     6061 tagccacagc gcctcccttc agcgtggtcc gcccccagac gccgctggga gcctcacgca
     6121 gagtctcccc acagaacgga tcgcgcagcg gcccggcgtg tttgccggcc ggaccggcgc
     6181 ggagcttgag caaggtcaag aatgacgccg ttcacgcggt ggtaccatcg ccccgaaggt
     6241 aatcccaacc tatggagtcg ggtatgaaca ccgcgccgcc gctgaccatc acgcccaaga
     6301 ccaccatcca caccctgctc aaggaatatc ccttcctcct ggacttcctc gcggagtacc
     6361 acccggagtt caagaagctg accaatccgg tgctgcgccg cacggtgggc cgcatggcga
     6421 cgctggacgc ggtggcggag cagggcaacg tgccgctgaa ccggctcacc gacgacatcg
     6481 cgaccgaggt ggagaagcgc accggcacgc gcccgcccgt ggccgacgtg gccaatgccg
     6541 agaccatcga cccggcgcgg ctcgccgagc tgcacgccat cgtcaaggac ctccacgccg
     6601 gcaagtcggt ggaggaggtc aagcctcggt tcgaggagct gatccaggac gtcgaggcca
     6661 ccgagatcgc cgccatggag cagaagctca tcgagggcgg cgtgccggac accgaggtca
     6721 agcgcctctg cgacgtgcac gtgcaggtgt tcgccgacgc gctcgagggc cacgccgcgg
     6781 tcaccgcgcc gcccggccat cccctggaca cgttccagcg cgagaaccag gcgctgctgc
     6841 aggtgacggc gtcactgcgc aaggtggccg aggcgatcgg cgagccgccg gacggcgccg
     6901 cgtgggcgcg cctgaagggc gcgctgagcg acaccgtgga gcggctcgcc gaggtcgaca
     6961 agcactacct gcgcaaggag aaccagctct tccccttcct cgaggaccac ggcgtcgagg
     7021 gtccgagcaa ggtcatgtgg gccatccacg acgacatccg cgccctcgtc aagcaggcgc
     7081 gggccaccat caccgaggac gacgcagcca tggcggtgag cacctgcctc gcgctggcca
     7141 agatggtcga cgacatggtg accaaggagg agaaggtcct gcaccccatg gcgatcgaca
     7201 cgctgagcga agcggagtgg gcgaagatcc gcgccggcga ggacgacatc ggctacgcct
     7261 tcatcgagga cgtgccggcg tggccggccg cggggacggc ggcggaggcc ggcgccgcag
     7321 gcgctccggc cggagcggcc gaggccggag cgctcgctgc cgccggcctg ctcgccctcg
     7381 acaccggaga gctgaacctc gaggagctca acctcgtgct cggcgtcctc cccatcgact
     7441 tccagtacgt cgacgagcac gacagggtgc gcttctacag cgaggggcac cggatcttcc
     7501 cgcgcagccc cggcgtcatc gggcgcaagg tgcagaactg ccacccgccg gcgagcgtgc
     7561 acaaggtgca acagatcatc gatgcgttcc gcgccggcga gaaggacacg gcggacttct
     7621 ggatcgagat gcagggcaag ttcctgcaca tccgctactt cgccgtcagg gacggcggcg
     7681 gcgcctaccg cggcgtcgtg gagacggtgc aggacgtgac cgcgatccgg gcgctggagg
     7741 gacagcgccg gctgctcgac tggtagccgg cgctccggcc ggcagcggtg cgatacgatg
     7801 accgctgcag gcaatcactc cgagaggcgg tcccatccca tgttgtggaa acacggcggt
     7861 ctcacgcccg aactggcgga cctggtcttc accttcctcc cgctcgacct cagcgtcgcg
     7921 gacgagaacg acgtcctggt ctactggaag ggcgacacct acaagacctg cgacgcgcgc
     7981 tacatcggcc gcgacgtgcg cgactgccac ccggaacaca gcatggcctg cctcgaggag
     8041 atcctgagcg cgttcaaggc cggtacgaag gacgtggccg agggctggga gcagaagggc
     8101 gagacgatga agcgcacgcg gtacatcgcc ctgcgcgatc acgacggcgc ctacaagggc
     8161 atcctcgagg tcaacgaaga cgtgacgagc gcgcgcgccc tggagggcga ccaggccctc
     8221 cccggctggt gacgcacgga cgcgtgaggg agtgaggaca tgacgaccct cgtcgccgcg
     8281 accggcgccg gcgcgtggct gctgctcatc ggcgccgcca tcgccgggct cgccttcggc
     8341 atgatcatga acagcgtcct gaccgggcgg cggaagaaga agtagagccg cggcgtcagt
     8401 cgacgcgggc gaacgcgccg cggcggccgt cgaacgtgag ctgcgtgagc gtgacggcct
     8461 gggcccggcc gccgcgcacg gcgaagaccg cggccgtccc cgaagccatg tcccgccaga
     8521 tgtcggcgct ccagggcgcg tagcgcaggg tctgcccgcg ccccaggcgg acgacgagcc
     8581 cgtcgcccgc gtccggccgc accgtcacgc gcccgtagta cctgttctcg tacacccccg
     8641 cgtacacgtc gcgcgcccgc ggcgcagggg cggccgccgg cgcctgcggc ggcagcgcga
     8701 ggccggccgc aggcacggac gcacgctccg ttgcgagcgc ggcctgctcg cgcgccagcc
     8761 aatcctcctg aggggcaccg agtaccgcga gatcggtgag cgtgcgcgcg atcgccccgc
     8821 cgagcgccgc gccctccggg taggcgttgg cgagcacgat caccgcgacg ccatcgtcgg
     8881 gcagcagggt cacggtggcg ctggagccgc tcgcgaggcc gccttcggcg ctcgccagca
     8941 gacgcccgtc gaacgtagac agcatccacc cgggcgcggc cgcctcgggc ccggacggcg
     9001 tgtcggccac ggccgtcgcc gggcgcagcg tctgcgcgag cagcccggcc ggggccacgc
     9061 gcgcgccggc gaccgccccg ccgttgagtt gcagccgggc gaacgtcacc agcccgctga
     9121 ggctcgcgct gacgccgagc gagggcgcga acacgctctc gtccgccggg tcctgcggca
     9181 ccatcgtccc tgccacgagc cggtgcggcg tcgccttgtc gacggcccgc aggaagccgc
     9241 gccacgtgag caccgtgccg tggtcgccga ccggctcgag cacccgctcg cgcaccaggc
     9301 gcgcccacga gcgacccgtg gcgcgctcgg cggcggcggc cgccgccgtg accatcccgt
     9361 cctggggcgc gaactcggcg cggaagccgg cggccggacg cagctgacgc agacgccgca
     9421 gcacctcggc gcgaccgtac ccgaacgcga gcagctcgtc gccggcgtgc gccggcatcc
     9481 cgctgcgccc ggccgtgagg tcgcggaacg tcgcctctcg cgtcgcccag gggtcgcgca
     9541 gccggaatcc gggccacgcc cgccgcaccg gctggtccca gccgagctcg ccctctccgg
     9601 cgagtgcggc cagcatcgtc gacgtatacg cgcgcgacac cgcgcccagc tggaagagcg
     9661 tgtcgtcgtc gaccggatcg ggacggccca tctcccgcag accgaagctg cgtgagtaca
     9721 tgacggtgtc cccggcgacg acggccacgg cggcgccggg gacgccgctg cgcgccacca
     9781 tgctcgcgac gagcgggtcg agggcgggga gcgccctgcg gacggcggcc gcggcggaca
     9841 cgcgccgcgc ggcggccgcg agcttcgccg cgcggcgtcc ggacagggcg ggtgcaggag
     9901 cggcggacgg tgcggccgtc ggcacggccg agggcacgtc ctggggaggc gccacctgcg
     9961 gcgacggcgc cgcggagggc gaggccggcg ccgaggcgac cagggtcgcc gtccgtgccg
    10021 gcgcggttga aggcgacggt cccgcggcca cgcggatgcc gcagccggcg agcagcgtcg
    10081 ccgcgccggc cgccgcgagc acgcccgcgc gccatctggg tctcacctca gcctcccgga
    10141 agctcgtccc cttgttcgcc gcggccgccg gcataccctc gccgccggcc gggtgtagac
    10201 tgcctcccac ggggggtcga ggagacggat gacgatggac ggagacacgg ccagcggacg
    10261 ggggcgccgt gcggcgaccg ccgctcgcga gaggagtggc tcgcggtgac gccgcacgtc
    10321 gaggaccgcg agcgcaccga catcgcgacc tgcctcgggg agatcgaccg ggcgctcacg
    10381 gatgtgctcg aggggcgcgg cgtccactac gtggccgagg cgacgtgctc tccggtcgca
    10441 ctcccgctcg tgcacaccgt caatcggctg ctccgccagc tggaggagat cgtccgcttc
    10501 gtgctcccgc tctcccgcgg cgagctctcg gctccgctcc caccgcccga caaccggatg
    10561 gcggcggcgc tcgtgcagat gcacgcgcgt ctcagacagc tcacgcggca gacccaggag
    10621 atcgcccgcg gcgactacac ccagcgcatc gacttcatgg gcgagttctc cgaggccttc
    10681 aacgccatgg tcgccctcct cgaggagcgc gaacgcacgc tcaccgacga gatcgcccgg
    10741 cgccggcagg ccgaggacga gctgcagcgc gagcgcgacc tgctggtggc gggtccggtg
    10801 gtcacgttcc gctggggagt cgacgacgac gggacggtgg agtacgtctc ccccaacgtc
    10861 agcgcctacg ggtacgcgcc ggaggagttc accagcggcc gccgcgtgta cgccggcatc
    10921 gtggatccgg cggacatcgg ctggatcagc gaggacggca acgacaagtc gagggccggc
    10981 ctcgactgct ggacgcagga gtaccgcatc atcgacgccg agggccagac gcactgggtc
    11041 cgcgacttca cgcacacggt gcgcgacgcc gacggcgcgg cgacggccta cgagggctac
    11101 atcatcgaca tcacccccca gaaggcggcc gagaccgcgc tccgccagcg cgaggagcag
    11161 ctgcgcatgc tctcgctcgc ggacggtctc accggcctgt acaaccgccg cggtctcttc
    11221 gcgctcggcg agcacgccat gcgcagcgcg cgccgccgtg ccgggggcct cggggtcgtc
    11281 tacgtggacg tcgacgggct caaagccatc aacgaccgct tcggccacgc ccagggcgac
    11341 caggcgctgt gcgcggtggc cgacgtgatc cgcgccagca tccgcgagtc ggacgtggtg
    11401 gggcgcgtcg gcggcgacga gttcgtggtc ctggccgagg acgatccctc cgtgacgcgg
    11461 gagctggtgg cccggctgcg ccgccgcatg gaccgcgcca atgagcggtc ggggcggccg
    11521 ttccgcatct cgctgggcat cggcgcagcc gactgggaac cgggcgagca ggtcaccctg
    11581 caggagctca tcgagcgcgc cggccagcgc atgcacgacg accggcggga gcgccggcgc
    11641 tgagccccgg tcactcgcaa caccagtggt cgccacgggt gagcgcggcg atgctcgcgc
    11701 gcctcgcggc gcgcgccgcc gtcgccgccc ccgccggggc gacgccggtc gaggccccgt
    11761 tcaccggcgc cgtgctggcc accgtcccca agggccgtcc cgccgacgtg gaggctgcgt
    11821 gcgccgcggc gcgagaggct caggtggagt gggcgcgccg ccccatcgag gagcgcgccc
    11881 gggtgttcct gcgctaccac gacctcgtga tcgcctgcgc gcagggactc ctcgacctca
    11941 tccagctcga gagcggcaag gcacgcatcc acgcgtacga agaggtgctc gacgtcgcca
    12001 tccaggcacg ctactacgcg cacgccgccg agagatacct gcgcgagcag cgcaagcagg
    12061 gcgccctccc gctgctcacg tccacgaggg tctatcaccg gccgcgcggg gtgatcggca
    12121 tcgtcacgcc gtggaactac cccttcacgc tcgcgatcag cgatgccctc cccgcgctcg
    12181 tcgccggcaa cggggtcgtc ctcaagcccg acacgcagac gccgctgacg gccctccggg
    12241 cggcggacct gctggaagaa gcggggctcc cccggggcct gctgcaggtg gtcaccggtc
    12301 gcgggtccga gctcggagag ccgctcgtcg acgccgtcga ctacgtgatg ttcaccggct
    12361 cgacggcgac ggggcgcacg gtcgccgccc agtgcggcgc gcgactcaag gactgctcca
    12421 tggaactggg cggcaagaac gccctgctgg tgctccccga cgccgcgctc tggagggcgg
    12481 tcccgggcgc gctgcagggc atcacgtcca gcagcggcca gctgtgcatc agcatcgaac
    12541 ggctctacgt gcacgacgcc gtctacgacg tcttcgtacc gcgcctggcc gagcgcctca
    12601 aggccgtgcg gctcgggtcg agcctcacgt tcgccgacga catgggctct ctggccagcg
    12661 ccgaccagct cgccaaggtc gcggcgcagg tggacgacgc cgtcggcaag ggagcccagg
    12721 tgctggcggg cggcagcgcc cgccccgacc tcggcccgta cttctacgag ccgacgctcc
    12781 tcgccggcgt caccgaggac atggagctgt gccgcgccga gaccttcggc ccggtcgcgg
    12841 ccgtctaccg ctgctcgtcg gtggacgaga tggtggcgcg ggcgaacgac agcgagttcg
    12901 ggctcaacgc gagcgtctgg acgcgcgacg tcgaggccgg tcgcgcgatc gccgcccgtc
    12961 tccagaccgg gacggtgaac gtcaatgagg cctacgcggc cacgtgggcg tcggcggcgc
    13021 cgatgggcgg ctggaagcag tcggggctgg agcgccgcca cggacggcac ggcatcctca
    13081 agtacaccga tccgcagacg gtctccgtcc agcgcctgct caacatcggt acgccgccac
    13141 tgctgagcca cggccagtac gcgacggtga tgacgcgggc cctgcgcctg ctcaagcacg
    13201 tgcccggcat caagtgacga gggtggaggc gctcggcgcc tccggagcgc gatctgggcg
    13261 agcgcctaga gctggaactc gtggcggcag tacgcgtcgc cgcggctcag cgcgcgcgtc
    13321 tgcgcgtgga ccgaggcgtc gacgccgagc atgccgcaga gcatgccgcg gtcgaacgcg
    13381 cagacgacgt ccgggtactc ctgggagagc tcccggtaga cgcagttgtg gacctcgatg
    13441 accgtcgcgc cgttgccgtt ggccgagagc gtcgcaccgt agccggcgag gtccatccag
    13501 tccatgaccg cgcgcggcga gagggcggcg ggagcacgcg tgtcgccgcc ggcgaactcc
    13561 tctgccgtct gctcgccgca ggtgcggccg agagccgccg cggtggtggc cgcgacctcg
    13621 ggctcgagcg tctcgtgcac caggcgcagg agcagccgcg ccagacgttc gtagcggcgc
    13681 ggcggcagca tgatctccag ccgcgcgtcg ctgaccgcgt agatcttggc cggccggccg
    13741 ccgccggcgc gccggcgcgt cgccgtggtg acgaggtcca gctcggtgag cctctccagg
    13801 tgagcgcggg cgacggtgcg gtgcaggccg aagacctcgg cgacctcact ggcggactgc
    13861 ggctgctcgc agccgcgcag gtgacggtag atcccgcgcc gggtgggatc gccgaacacg
    13921 tcggcgagcc tgcccagata caggccgtcc ttatcgtacg aggtctccac gaatgcgaca
    13981 ctatcgcact tcctttcaca atccaaggct tctgatggtc gttgagcaca caatccggcc
    14041 atcacctgag aggggtgtcc tgcgacgtcg gcgccgcggc gatgcggcct tcccgacgtt
    14101 atggaagtcg gccccgggga taagcagcag acagcggccg caagcccgca ggtgcaccga
    14161 cgagaggagc agccatgatc aaggtgagga gcttcgccac gccgatcaag atcttcgcca
    14221 cgatgcgcga actgcacgac ctggatgcgc aggtagccga cttcctgaac gaggagggcg
    14281 ccgacaaggt gttctcgatg agcgacagca cgacggtggg cgagcacggg gagaccatcg
    14341 gcctcatccg cgcggttgcc taccgggtgc cggactgagc gccgccgtgg acgccgccac
    14401 ggggcaccct gagatgagcc acgacgaaga caccgcgggc gagggcccgg cccacccgat
    14461 cccacccgag gcgcgagcgg cgaatctggc gctctggaac cactggacca cggtccacga
    14521 gaagagcgcc ttctacgacg tcgagggctt caaggcggga gcgacctccc tctggccgat
    14581 cgaacgcgag gagctcggcc ccttggtgca cgagggcacc tccctgctgc acctgcagtg
    14641 ccacttcggg ctcgacacgc tcagctgggc gcggctcggc gccggcgtcg tcgggctgga
    14701 cctgagcgac gaggccatcg ggctggcgcg gcgcctggcc gccgagatcg gcgtggccga
    14761 ccgcgccgag ttcgtctgcg ccgacctcta cgacgcggac gcccacctcg gcggccggct
    14821 cttcgacatc gtgttcgtca gctggggcgc gatcgagtgg ctcccggacc tggagcgctg
    14881 ggcgggcatc gtcgcgcggc acctgaggcc cggcggcacc ttctacatgg ccgagatcca
    14941 cccgttcgcc tacgccctgg acgaggtgcc gggcgaacac gacgtacagg tcaagtaccg
    15001 gctcctgccg gcgcccgagg cgccggacgt cgatccggtg gagggcagct acgccgaccc
    15061 cggggcggac acgacgggcc tcaccgccta cggctggacg cacagcctcg ccgagatcac
    15121 cggggcgctg atcggcgcgg gcctccgcct ggagcacctc cacgagttcc ccacctcccc
    15181 gtcgccgttc tggagctgga tggtccgtga cgaggaccgc tggtggtggc tgcccgacgc
    15241 cgagggcggg ctgcgcaagg acctgcccct gagctactcg ctgcgcgcct ccaagcctgc
    15301 caacgcggag ggctgacgat gctcgacgac tacctcaagg cgaatctggc gagctgggac
    15361 gaggcggtcg ggctgcacgt cggctcggag ctgtacgacg tcgccgggtt caaggccggt
    15421 cgtaccgcgc tgagcgcgat cgagctcgac gagctcggcc cgctggtcgg tgaggggacc
    15481 aggctcctgc acctgcagtg ccacttcggg ctcgacacgc tgagctgggc ccgccgtggc
    15541 gcggaggtca ccggcgtcga cttctccggc gagggcatcc ggacggcgcg cgtgctggcc
    15601 gacgaggtcg gcctcgcctc gcgcgccacc ttcgtccaga gcgacataga gagcctgccg
    15661 gacaagctca ccgggcagtt cgacgtggtc ttcacctcct ggggagcgct catctggctg
    15721 gccgacctcg agcgctgggc cgacgtgatc gcgcacttcc tgcggcccgg cggtgtgttc
    15781 tacatcgccg agttccaccc ctacgcgttc ctgctcgccg acgatgcgac cccggactct
    15841 ctccgcgtcg gctacccgta cttcatgcac ggcgtcccgc agcgcttcga cgaggacggc
    15901 gactacgccg accccgacgc caaggtgaag aacacggtga cgttcgagtg gctgcacggc
    15961 ttcgcagaga tcgtggaccc gctgctgcgc cgcggcctgc gcctcgactt cctgcacgag
    16021 ctgccctaca ccgtacccgg actgcaactt cccttcgtcg agaaggacga ccgcggcatg
    16081 cagcgcgtca agggccacca cgaggacttc cccctcagct tcaccctcaa gatgacgcgg
    16141 gaggggcgga cgtccggccg cgccgggcgg ccgccgggcg accgtgcgcg cctgctcggc
    16201 gacgaggacc tgctggcgat cctcgaggag gcgatcgccg acgagcgcgc cgcccaggag
    16261 aagtaccgcc gcgggctcga gcgctgcgcc gaccccgcgg cgtgcgaggt cttcgagcaa
    16321 ctgctccgcg aggagcaggc gcacgagcgc gtgctcgagg cgcgctacgt ggaggtcaag
    16381 aagcgcgtcg ggctcagcgg ggccggccgg accgactgag cgggcccggc tcgagaccgg
    16441 cggccgcgca acatttcttc acagccgcgc gcgtcgtccg tcttgaccgc gccgcgcgct
    16501 cgtcgtattc tttttgtgcg ccatcacagt caaaacagtg atcgcgtgtc ccgtcagggg
    16561 gacaaagacc ggtgctatcc caccagcacc gaggcccctc gaaggcggcg cctcccctcc
    16621 aggcgccgcc ttcacttctg tgcgcgcggg tccgccccgc gcgcgagctc gcgcagaagg
    16681 ccggggatgc cggcgcgcgg caccacgagg tcggccgccg cgcgcaccgg ctcgtcggcc
    16741 tccgccaccg cgacaccgag cccggcccag gcgagcatgt ccacgtcgtt catgccgtcg
    16801 ccgagagtga ccacctgctc cctggcgacc ccgagccgcg cgcacagcca ggcgagggca
    16861 ccgctcttgc tcaccgacgc gtcggcgaac tcgatgtact ccggctgcga gcggacgacg
    16921 tacagccgcc cgcgccagcg ctcctgaagg cccgcgagga cggcgtccgc gtccgccggc
    16981 gacgtgacca gcacgagctt ggtcggcggc cgccggacca cctcggcctc gaggtcggcc
    17041 accgtgtgga tgccgacctc cacgtgctcg gcgtagcgcc gcgcccactc cgtgacctgc
    17101 tccacgtaca ggttgtcgtc gatgtaggcg ttgagctgcc gccccatgcc cgcgacatgg
    17161 cgcaccacct cggcggccgc ccggccgtcc atgggcacgt ggcgcagcca ctcgccggtg
    17221 ccgagatccg cgacgagggc gccctggtag cacacgaccg gccccgcgct cacgcccagc
    17281 cgcgcggcca cccggcgggc gctctggaac atgcggccgg tgcagacgac cgcatggatc
    17341 ccggccgggg cgaggtccgc aacggcttcg ggcagcccct ccgcccacga gaggtcccgt
    17401 gcgatgaggg tgccgtcgag gtccgccgcc acgagggctg ggcggaaggc aggcggcagc
    17461 acggcgcctc cggagctcac cggccgccgg tcaacggatt gcccgcactg cggtcacggg
    17521 tacagtcaag ccgtatcgca cagtccgata cgcggagaag agcgacaccc cagcacccac
    17581 ggggcgcctt cttacgaaga cctggtgatt cccaggtctt cgttttgcca tcaggccttc
    17641 cgcgccggag cgcgcatcga cgtcacgatg aggtcgagcc gcaccaggtc cacggccgcg
    17701 acccggcgca tgtcgtcgcg gaacgcctcg agcacggcgc cgtggacgtc acgcaggtcg
    17761 cagacgggga cgtccgcctc cacgcgcacg ctccaccccg catcgtccat gcgtacggcg
    17821 atctcggtca cgcgggatcc cgggagcatg gcctcgagcc ggcggcgcaa ggccttgcga
    17881 agggccggga ccgagaggcg ggcggtcccc tcggggaccg agaactcgat gacctgcggc
    17941 gtggcgcccg gcgagacctg acggtacgcg aacacgatga gcaggaccgt cacgacggtc
    18001 atgacggcgg ccgcgacgat cgtcgccgtg gacgagcgga tcgtcaggtc cgcccaccag
    18061 gcggcgatcg gccaggagac gccggcgtcg acggccatcg ccgcctggcg caccagggcg
    18121 ccgccggcga gcagcagggt cacgatgccg acgatgacgg cgatgacgcg cgcgagcgga
    18181 gtcacgggag atacctcacc agttgcgtca ccttgagcac cttgacgcgc acgtccaggc
    18241 gctcgagctc gcggccggtg agccgcgcca cctggcgccg cacggccgcg tccacggcgc
    18301 cccccaccag gcccgcgtcg gcgagcggac gcgcccacac cgtcacccgg ccgcgcaggt
    18361 ccgcgccgcg cttgaacaac tcgacgtcgg cgcgcaccac gtcggggtga gagcggccgg
    18421 aggccgacgc ggccggccgc tcgagagctt cgttgggcac caggacgccg ccgtcaccgg
    18481 ccggggaagc gagccacagg gagtcggagt cgtgacgcaa caccagccag acaagcgtcg
    18541 cgagcacgac catggcgagc agaagcagcc aggccagcag tgcgtgcccg ttcggggtga
    18601 tgtacgcgaa cggagagaag tcccagtcga gggccacggc gagcagccgc agggcgaaga
    18661 gcagcgcgac gagacccccc gccagcagca ggaggaacgt cgcgacgcgg gcgatggcgc
    18721 cggccgccgc tccggcgggc cggcgtctct cagcccgcgg cgccagcctg gtcctccggg
    18781 aacaccacgt cggtgacgag cacgttgacg gcgcgcaccg gcaggccggt ggtggcctcg
    18841 acctgctcgg agaccgcctt gcggcaggcc tccgccacgg ccggcacgct cgcgccgaac
    18901 acgatgctca tggtgatgtc gatgtcgacg ctcccctcgc gcaggtcgac cgagatgccc
    18961 ggcgtgccct tgtcgccgcg cagacccgcc agcgcccggg cgctggtgcc gcccagggcg
    19021 tggatcccgt cgatgccgcc gatcgccttg aaggcgacct tggcgaccac gctgtcagag
    19081 atggtgacgc cacccccgcc gggggccgca gtgggcttcg gggtttcgct catggctctc
    19141 ctccgtcttc ggtcgtccgc gctcatcaac gagcgacggc cgcggccgtc gtgcctactg
    19201 tacccggcgc gggcgccggc gacgaatcag ggccgcggcg tcagctgcac ttgctgtagc
    19261 tgcagctcgg gtcgttgcac acggcgcagc cgccttcgtg acgcatggtg gagccgcatt
    19321 cagggcacgc accctgcgcc acctcgacca gggagagctg cggctccggc gtggcgctcg
    19381 ccctcgcggc cgcaccggcg acgtaccgtc gctccagggc cttgccgatg gcgtcggcgc
    19441 agctgagcgt ggcgttgggg cccatgccgt agggcacgtg gcagcggatg cccttgagct
    19501 gctcgacgat ctcctgcgcc gaggcgccct tcttgagagc gagcgagatg agccggccca
    19561 gcgcctcggt gttgctcgag gcgcacccgc cggccttgcc catgttggcg aagacctcgc
    19621 gcgggccgta ctcgtcgtcg ttcatgatga cgaagagctt gccgcagccc gtgtccatgc
    19681 gctgcgtctc gccggtgagc agctgcgggc gcgccggccg cgtggtggcg atgcccaggc
    19741 caggctcggg ctcgcgcttc ttcttggtct ggccgacgtt gagcacctgc tcgtcgcggc
    19801 tgccgtcgcg gtagatggtg acgcccttgc agccggtgcg gaaggccatg cggtagacgt
    19861 tctccacgtc ctccacggtg gcctcgttgg ggaagttgac cgtcttggac acggcgttgt
    19921 cggtgctctt ctggaacgcc gcctggagct tgatgtgcca ctcgggcgtg atgtcgtggg
    19981 ccgtggcgaa gacctgctgc acgtcctgcg gcacaccgtc ggtgtgacgc gcggagccga
    20041 cctcggcgat cctcttcatg agctcgtcgc tgtagaagcc gcgctcgcgg gccacctgct
    20101 cgaagtaggg gtggccctcg accatgcggt cgtcgtccag cacgttggcg cgcacgtagc
    20161 tgagcgcgaa gatcggctcg atgccgctgg agcagccggc gatgatgctg atggtgcccg
    20221 tgggcgcgat ggtggtgacc gtggcgttgc gcacccgggc gccggccggc gagtcgtaga
    20281 tcgatccctc gaaattgggg aacacgccgc gctcgcgcgc gaggtcctcg ctctccgcac
    20341 gcgcctcgtc gtggatgaat cccatgacct ctccggcgat acggcaggcc tcgtcagagt
    20401 cgtagggcac ctgcaggcgc agcagcatgt cggcgaaccc catgacaccg aggccgatct
    20461 tgcggttgcc cttggtcatc tgctcgatct cgggcagcgg gtagcggttg acgtcgatga
    20521 cgtcgtcgag gaagcgtacg gcggtgcgca cgatgcgtcc cagggcgccg tagtcgatgt
    20581 ggccgtccgg gacgacgcgg gacaggttga tcgagcccaa attacaagat tcgtacggca
    20641 agagtggctg ctcaccgcat ggatttgtac tctcgatatc gccgagctgc ggcgtggggt
    20701 tgtcctcgtt gatgcggtcg atgaagacga tgccggggtc gccgttcttc caggcgagat
    20761 tgaccatgtt cttgaagacc ttgcgcgcgc tgagcgtgcg caccgtctcg ccggagcgcg
    20821 ggttgaccag cgcgtactcc tcgtccgcct cgacggcgcg catgaacgcg tcggtgacgg
    20881 cgaccgagat gttgaagttg ttgaggcggt cgtcgtcctg cttggcggtg atgaagtcga
    20941 gcacgtcggg gtggtcgacg cgcatgatgc ccatgttggc gccgcggcgc gtgccgccct
    21001 gcttgatggt ctcggtggcg gcgtcgaaca cgctcatgaa cgagagcggg ccgctggaga
    21061 cgcctctcgt gctgttcacc acgtcgttgt gggggcgcac gcgcgagaag gagaagccgg
    21121 tgccgccgcc gctcttgtgg atgagggcgg tgttcttgat ggcctcgaag atggactcca
    21181 tggagtcgcc cacggggagg acgaagcacg cggagagctg gccgagctcg cggccggcgt
    21241 tcatgagggt ggggctgttg gggaggaact ccaggctcgt catgagctcg tagaaggtgc
    21301 cctcccactc ctcgaccgga gcggcggggt tgtacaggcg ctcggcctgc gcgacggcgc
    21361 gcgcgacgcg ccggaacatc tcctcggggg tctcgagcag ttcgccgttc tcgccctttt
    21421 tgaggtagcg gcgctgcagc accttgagtg cgtttgcgga cagctcgatc actgccttcc
    21481 ccgtttcagt catgccccgt tctcccctcg gtcgtcaccg cacgccgcgg cgtggtgaag
    21541 atgatctcgt cgatgaagct ctcgccggcg tcgatgcccg gaaggggctc ctcgcccggc
    21601 agcggctcga cgcctgccag ttccagctgc gccagctcct gctggaactc ctcgatgtcc
    21661 tcgaactgcc ggtacacgga ggcgaaccgg atgtacgcca ccttgtcgat gtcgcgcagg
    21721 tggcgcagcg cccgctcgcc gaccatcctg gtcgtgaccg ggcgccccgg cacgcggcgc
    21781 aggtcgttct cgatgtcgtc gcaggcccgc tcgagccggt ccagggcgat gccgcgcttc
    21841 tcgcacgcgc gcagcaggcc gttgaggacc ttgtggcgcg tgaacagctc ttctctgccg
    21901 tcgctcttga tcaccacgag cggtacctct tcgatgcgtt cgtaggtcgt gtagcgccgc
    21961 tcgcaagcga ggcattcgcg gcgacgcctg atcgcgtcgc ccgcctcgga gtcgcgcgag
    22021 tcgacgactt tgctctcatc gctgctgcag aatggacaac gcataggaat ccgacctcac
    22081 cacaagatgt agtgcccccg tcagtgcgga caccactata gtgtattcgc gcccgcccgc
    22141 aaccgtcctt ggcgccgccg ttcgagcgat tcgtgactgg cgaccctcac cctctactcg
    22201 agagtgatgg tatgtgccgc agagcacacg gccggcccgc gcggagcacc gtaccaagcg
    22261 acccggacgg aggggcgcgg cgggcgctat acttgccgca ccgtgaacgt catcgtcctc
    22321 cccacttaca acgagcgcga caacctgcgt cacgctctcg gccgcatccg cgaggtcgcg
    22381 gacggccatg gggtccagct gcacacgctc atcgtcgacg acaactcgcc cgacggcacc
    22441 ggcgccctgg ccgacgagct ggcgcgcgag gtgcccgacg tcagcgtcat gcaccgggcc
    22501 ggcaaggagg gtctcggcaa ggcgtacatc gccgggtttc gcgaggccct cgccatgggc
    22561 gccgagcgca tcttcgagat ggacgccgac ctcagccacg acgccaccta cctgccccac
    22621 ttcctccgcc tcatcgacca gggggcggac ctcgtcctcg ggtcgcggta cgtgagggga
    22681 ggcggggtcg agaactgggg cctctcccgc aagatcatca gccgcggcgg ctgcctgtac
    22741 gcgcagacca tcctcggcct gccgctgcgc gacctcaccg gcggctacaa atgcttccgc
    22801 cgcgcggtcc tcgagaccgt cgacctcgac accgtcgaca ccaagggcta cggcttccag
    22861 atcgagatga cctaccgcgt ctacaagctg gggttcaagg tggtcgagct gcccatcatc
    22921 ttcgtcgacc gcaaggtggg cgagagcaag atgagcaacg acatcgtggt cgaggcgatg
    22981 aagaacgtct ggaagctgcg cttcagccgc gtccccaccc ggcggaccgc ctgagcccgc
    23041 gccgggcgct cctccgccgt gacctcccca gcacagcccg ccggcgtcct cgcgatcgac
    23101 gtgggcgccg gcaccaccga catcctcttc acgcgacccg gccagccgct cgagaacgcg
    23161 gtcaagctgg tcgtgccctc ggcgacccag gtcgtgggag cgcggatcgc cgccgcgacg
    23221 gcccgcggcc agacggtggt cttccgcggg ccggtcatgg gcggcggcgc cgtcaccgcg
    23281 gcgatgaagg cgcacctcgc cgccgggcag gcgttcgtgg ccaccgagac ggcggcggcg
    23341 agcttcgccg acgatctcga ccgggtgcgc gcgctcggcg cgcgcgtggt gggcgaaggc
    23401 gccgcggacg aggtgctgcg gcgcctgccg gccggcgccg ccgtggaggt cgcctccggc
    23461 gacctggacg cggcggcgct cacccaggcc ctgcgtctgc tcggcgccga gccggcgttc
    23521 gccgccgtcg ccgtggccgt gcaggaccac ggctacgcgc ccggcggctc caaccgcgtc
    23581 ctgcgcttcg cgctgtggca gcaggcggtc gccgagcggc ggccgatcgg cgagctcttc
    23641 tacgcgccgg tggccgtgcc gccctcgctc acccggctgt gcgccgccgc ggacgcggcc
    23701 acggccctgg ccggcggcgc gcccgcgctc gtggccgaca ccgggccggc ggcgctctac
    23761 ggcgccctgc cgcgcgaagt ggagaacgcc gtcctcgtca acgtcggcaa cggccacacg
    23821 gtgtgcctgc tggcgctgga gggccgcgtg gccggcgtgt tcgagcacca cacctcggcg
    23881 atcgacgggc ccggcctcga gctccgcctg cgccgctggc tggccggcga cctggagagc
    23941 gaggaggtgc gcgccgacca cggtcacggc gccgtgctcg ccccgggcgc cgcggacggc
    24001 gaccccctcc gcctgccgct cgtcgtcacc gggccacgcc gcgaactgct cgccggcagc
    24061 cagctgcccg tcgagttcgc ggcgccgcac ggcgatatga tgctcaccgg ctgcttcggc
    24121 ctgctgcgcg cgctgtgcga gcggcatgcg ggcctgcgcg aggagccggc agcgtgagcc
    24181 gcccgctctc caccgggctc gtcgtgccgg tcgacgctgc gcggcagctc gtcgccccct
    24241 ggctcgaccg cctcccggcc gccgggcgcg cgctgccgcc gcacgtcacc gcgctctggc
    24301 cgttcctgcc cgcagtggcg gccgacgacg ccgtggagcg ccggctccag gagctgctcg
    24361 ccggcgcccg cgcctttccc ttcacgctcg agcgcgtcgc cgggctggcc gacgccgtcg
    24421 tcctcgtggc cgagccggcc gagcccttca ccgcgctcac gcggctgctc tgggacgcgt
    24481 ggcccgagtg cccgccgttc ggcggcgcct acgacgacat cgcgccacac gtcgtggtcg
    24541 cgatcgaccc gtcgacggct gaccgcgccg ccatcgaggc gacgctcgcg ccgcgcctgc
    24601 cgctggcggc ccgcgcggcg gaggtgctcc tcgtggaaga ggtggagagc ggcgccgtgc
    24661 gcgtccggcg gcgcttctcg ctcggcgcct agagcggcaa ctcccgccct gcgccgagct
    24721 cccttgcgcg gcgcagcgcg agctcgccgg tcaccacgtc ttcgagcgcc atcccgagat
    24781 tgagacagaa caggcgctcg tccggcgtcc tgcggccgtc gacgaggccg gcggccagct
    24841 cgccgagatc gccggcgacg tcgccgggga tgcccgcgag gcgcacgccg gccgcctgcg
    24901 tcgcccacac cgtcgccgtg tcgtcgcagt agaaccggtc gcactcgctc atggccggcg
    24961 cactccaggc ggcgtcgtag tcgagggcca ccgccagcgc cccgggctcg agcagcccgg
    25021 ggccgagcgg cggccggatg tccgcggtca tggtgatggc cgagacgacg acgccggcgc
    25081 ccctcacgac gtcctccggc gcggcgaccc tcgtgaagcg caggtccggc agttccgcgc
    25141 cgagggcgac ggcgaaggcc tcgccggccg cggtggtcgc gtcgtggcag cgcacctccc
    25201 gcaactcggg cagtacggtc gcgagcgccc gcacggacgc gcgggcctgg acgccgcagc
    25261 cgagcacgcc cacggtcgcg gcatccgggc gcgccaggtg gcgcgccgcg acacccacac
    25321 tggcgccggt gcgccacgcc gtcacggtgg ccgcgtccat caccgccgcc ggcagccccg
    25381 tcaccgggtc gctgagcacg accagaccgt tggtgatcgt caagccgcg
//
LOCUS       DASUMX010000185         2566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_641978, whole genome shotgun sequence.
ACCESSION   DASUMX010000185 DASUMX010000000
VERSION     DASUMX010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_641978"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..915
                     /locus_tag="VFZ86_09240"
     CDS             <1..915
                     /locus_tag="VFZ86_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642506.1"
                     /translation="VASPVFDGATIEEIDELLIRSSEETPDNPVEFKINRKEPAGRQC
                     SGKVWLYDGRTGERFDNKVTIGYMYILKLLHLVDDKIHARSTGPYSLVTQQPLGGKAQ
                     FGGQRFGEMEVWALEAYGAAYVLQEMLTIKSDDTVGRVKAYEAIVKGENIAKPNIPES
                     FKVLMKEIQSLALDMSVQSVEGDVLEVREEDDDLLRAAEELGIDLSAGLRRQQAEAGE
                     LAQVGVDSSALRDVIGAGTEPVEEGDGEPVVDDDSAAAAQDLLAAAAALASDVDLTAV
                     GGELAGLDDILDLDAADLEPDDADSDED"
     gene            941..>2566
                     /locus_tag="VFZ86_09245"
     CDS             941..>2566
                     /locus_tag="VFZ86_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEX5642507.1"
                     /translation="MIDINNFDSIRIGLASPKQIETWSKGEVTKPETINYRTLKPERD
                     GLFCERIFGPTKDWECYCGKYKRVRYKGIICERCGVEVTRAKVRRERMGHIRLAAPVS
                     HIWFFKGVPSRMGYLLDIAPKELEKVLYFGGSYVVISVDAERRDNDLLMLQEKIDEIK
                     ARYDLYAEARRGAIEAMHDEVVEVLDGARDIDDFHRDSDPEDEFGYFNYFDIYESYRR
                     IAMNAAALGPDEIKLQRTKTDKLRDDYIDRGFKNVEHAKEQIDKAWDQFKTIAPRDVI
                     GDESLYQQMERMFGRESGYEPESFGVYFTGGTGAEAVRELLKRVDLEAEAAELRETIQ
                     TAKGQRQARAVKRLKVVSAFLRSGNHPEWMILDVIPVIPPELRPMVQLDGGRFATSDL
                     NDLYRRVINRNNRLKRLLDLQAPDIIVNNEKRMLQEAVDALFDNGRRGRAVTGPGNRP
                     LKSLSDMLKGKQGRFRQNLLGKRVDYSGRSVIVAGPHLKMHQCGLPKMMALELFKPFI
                     MSRLVGRKMVQNIKAAKKMVESMVPEVWDILEEV"
BASE COUNT          489 a          885 c          835 g          357 t
ORIGIN      
        1 gtggcgtccc cggtgttcga cggcgccacc atcgaggaga tcgacgagct gctcatccgc
       61 agcagcgagg agaccccgga caacccggtc gagttcaaga tcaaccgcaa ggagccggcc
      121 ggccggcagt gctcgggcaa ggtgtggctc tacgacggcc gcaccggcga gcgcttcgac
      181 aacaaggtca ccatcggcta catgtacatc ctcaagctgc tgcatctcgt ggacgacaag
      241 atccacgcgc gcagcaccgg tccgtactcc ctcgtcacgc agcagcctct gggcggcaag
      301 gcccagttcg gcggccagcg cttcggcgag atggaggtgt gggcgctcga ggcgtacgga
      361 gccgcgtacg tgctccagga gatgctcacg atcaagtcgg acgacaccgt cggccgcgtc
      421 aaggcctacg aggcgatcgt caagggcgag aacatcgcca agcccaacat ccccgagtcg
      481 ttcaaggtgc tcatgaagga gatccagagc ctggctctcg acatgagcgt ccagagcgtc
      541 gagggcgatg tgctggaggt ccgcgaggag gacgacgatc tcctccgcgc cgccgaagag
      601 ctgggcatcg acctcagcgc cggcctgcgt cgccagcagg ccgaggccgg cgagctcgcg
      661 caggttggcg tcgactcttc cgccctgcgg gacgtcatcg gcgccggcac cgagccggta
      721 gaggagggtg acggcgagcc cgtggtggac gacgactccg ccgcggcggc tcaggatctc
      781 ctggccgccg ccgcggccct ggcgagcgac gtcgacctca ccgccgtcgg cggcgagctg
      841 gcgggtctcg acgacatcct tgacctcgac gccgcggacc tcgagcccga cgacgccgat
      901 tccgacgaag actgaccccg cctaggcaga gggagcaata gtgatcgata tcaacaactt
      961 cgactcgatc cggatcgggc tggcatcgcc caagcagatc gagacgtggt ccaagggcga
     1021 ggtcaccaag cccgagacga tcaactaccg cacgctcaag cccgagcgtg acggtctgtt
     1081 ctgcgagcgc atcttcggtc ccaccaagga ctgggagtgc tactgcggca agtacaagcg
     1141 cgtgcgctac aagggcatca tctgcgagcg ctgcggcgtc gaggtcactc gcgccaaggt
     1201 gcgccgcgag cgcatgggcc acatccggct ggccgcgccg gtcagccaca tctggttctt
     1261 caagggcgtg ccctcgcgca tgggctacct gctcgacatc gcccccaagg agctggagaa
     1321 ggtcctctac ttcggcggca gctacgtggt gatctcggtc gacgccgagc gccgcgacaa
     1381 cgacctgctg atgctccagg agaagatcga cgagatcaag gcgcgctacg acctctacgc
     1441 cgaggcgcgc cgcggcgcga tcgaggccat gcacgacgag gtcgtcgagg tcctcgacgg
     1501 cgcccgcgac atcgacgact tccaccgcga ctccgacccc gaggacgagt tcgggtactt
     1561 caactacttc gatatctacg agtcctaccg gcgcatcgcc atgaacgccg ccgccctcgg
     1621 cccggacgag atcaagctcc agcgcaccaa gaccgacaag ctgcgcgacg actacatcga
     1681 ccgcggcttc aagaacgtcg agcacgccaa ggagcagatc gacaaggcct gggaccagtt
     1741 caagaccatc gccccgcgcg acgtcatcgg cgacgagtcg ctgtaccagc agatggagcg
     1801 catgttcggc cgcgagtccg gctacgagcc ggagagcttc ggcgtgtact tcaccggcgg
     1861 caccggcgcg gaggccgtgc gcgagctgct caagcgcgtc gacctcgagg ccgaggccgc
     1921 cgagctgcgc gagaccatcc agaccgccaa gggccagcgc caggcgcggg cagtcaagcg
     1981 cctcaaggtc gtctccgcct tcctgcgcag cggcaaccac cccgagtgga tgatcctcga
     2041 cgtgatcccg gtcatcccgc cggagctgcg ccccatggtg cagctggacg gcggccgctt
     2101 cgcgaccagc gatctcaacg acctctaccg gcgcgtgatc aaccgcaaca accgcctcaa
     2161 gcggctgctc gacctgcagg cgccggacat catcgtcaac aacgagaagc gcatgctgca
     2221 ggaggccgtc gacgcgctgt tcgacaacgg ccgccgcggc cgcgcggtga ccggccccgg
     2281 caaccgcccg ctcaagtcgc tcagcgacat gctcaagggc aagcagggcc ggttccgcca
     2341 gaacctgctc ggcaagcgcg tcgactactc gggccgctcg gtgatcgtgg ccgggccgca
     2401 cctcaagatg caccagtgcg gcctccccaa gatgatggcg ctcgagctct tcaagccgtt
     2461 catcatgagc cgcctcgtgg gccgcaagat ggtccagaac atcaaggcgg ccaagaagat
     2521 ggtcgagtcg atggtgcccg aggtctggga catcctcgag gaggtc
//
LOCUS       DASUMX010000186         5186 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1071107, whole genome shotgun sequence.
ACCESSION   DASUMX010000186 DASUMX010000000
VERSION     DASUMX010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 5186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5186
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1071107"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="VFZ86_09250"
     CDS             <1..458
                     /locus_tag="VFZ86_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642508.1"
                     /translation="NRYSSRGLLLWNRYDQRFAAGYTLREALDAYIARMLHTRTSVAP
                     DTRAREYFEKTIALLNEHGTTPVIVIMPIHPRVLRVMKEHDMGGERQQLRDYLAALGE
                     TASIKVLDFTTIRSFNGEAGWFYDGVHINRRNTNRVITAVKAKAGEYLK"
     gene            468..1415
                     /locus_tag="VFZ86_09255"
     CDS             468..1415
                     /locus_tag="VFZ86_09255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX5642509.1"
                     /translation="MAAGQGPMQKKAPAAGGDAAGGGAAAIRREVVALLGQVLAHDLT
                     QLSAALTYYTVLSLLPALIVIVSLLGMVGLQPDTVHALLDTVGELGAPWAAEFVSTTL
                     ESVLRSQNSGVVFGISLLFSLWAASAYVGSFMAASDRIYEIVRRRPFWTGIPLRLGLA
                     LALLVLLSVTAAAVALVGPFGQWVADATGIGRGPLHLWTWAKWPLLIALGLLLFTLLY
                     KFTPSRGQPALWWLLPGAAVGIALWIGASALFSLYLDNFASYNRVYGTLGAAVAFLVW
                     AWLLNIALLVGVEVNREVESRRPGRSGAEDGAGRAAASV"
     gene            1471..2400
                     /gene="nadA"
                     /locus_tag="VFZ86_09260"
     CDS             1471..2400
                     /gene="nadA"
                     /locus_tag="VFZ86_09260"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEX5642510.1"
                     /translation="MPELNALQKELRRLAAERDVVILAHNYQRPEVQDAADHTGDSLE
                     LSRIAAKDPHQVILFCGVHFMAETAYILAPQKTVLMPDKRAGCPMADMVTVAGLRKLK
                     AEHPDAVVVAYVNTSADIKAETDVCCTSANAVQIVERFPKEQKIIFVPDRNLGDYVAR
                     QTGREATMILWQGWCHVHDDFRAAEIEEIRAANPGSVLMAHPESRREVLDIADVVTST
                     SGMLRYPQTSDARTFIVATETGLLYRLGVLYPDRTFLPASNQAVCPNMKRTTLEKCVA
                     TLSDEWDDVLEHEVVVPEPIRVKALQAVERMLA"
     gene            complement(2421..3377)
                     /gene="cysK"
                     /locus_tag="VFZ86_09265"
     CDS             complement(2421..3377)
                     /gene="cysK"
                     /locus_tag="VFZ86_09265"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEX5642511.1"
                     /translation="MKIAEDITALIGGTPLVRLRALSERSGATIVGKLESFNPGGSVK
                     DRIGLSMIDAAEEAGLISPDRTTIVEPTSGNTGIALAMVAAGRGYRTVLTMPETMSVE
                     RRNLLRAYGAELVLTPGAQGMKGAIARADELGREIEGAFVPQQFENPANPEVHRRTTA
                     EEVWGDTDGEVDVFVAGVGTGGTVTGVGEVLRERKPDVRIVAVEPADSPVLSGGQPGP
                     HKIQGMGAGFVPKVLNTQVYDEVVQVTNEQAFEAARELAAEGILVGISAGANAYAAYL
                     VGSRPENRGKLIVTVLCDTGERYLSTVLFAEPEPAAEGSTGI"
     gene            complement(3403..3843)
                     /locus_tag="VFZ86_09270"
     CDS             complement(3403..3843)
                     /locus_tag="VFZ86_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX5642512.1"
                     /translation="MNVSSKSRYALMALVELDLRTRGEQKPVRLTDLAAERRIPEQYL
                     EQLFAALRRAGVLTGHRGVGGGFTFARRPDRVSVLAVVEALDGAPDFEPSAREMSDPR
                     ERATAGAVWRAAGDAYQDVLARTTVSDLAERELQLGAGGPTYEI"
     gene            3989..4255
                     /locus_tag="VFZ86_09275"
     CDS             3989..4255
                     /locus_tag="VFZ86_09275"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEX5642513.1"
                     /translation="MPAITVSLPAALTAPQPARDVACEASTVDEALRAVVEQAPQYAQ
                     RIFYGDRLLVVVTLNGRHLPPSTVRETELTTGDRVEVLLPVAGG"
     gene            4379..4867
                     /locus_tag="VFZ86_09280"
     CDS             4379..4867
                     /locus_tag="VFZ86_09280"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HEX5642514.1"
                     /translation="MRLWATRETRSELMLAALRVLMGVIFLAVWADNLIKGYYSPSGW
                     ADFVQQYADTTKVGLYADLLNSVMIPNATVFAYGQFAIEFVVCGLFLVLGLFTPVSAL
                     LGAAFQLNLLIATSGTGEWPGTYIIMCLLLVAVGLAQSGRTLGLDALLARRDPHPRLP
                     VY"
     gene            complement(4905..>5186)
                     /locus_tag="VFZ86_09285"
     CDS             complement(4905..>5186)
                     /locus_tag="VFZ86_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642515.1"
                     /translation="RLLFGLRLSDDGPFRAVRTDLLERLRLEERAYAFPTEMAVKARL
                     SGARIAVRDTRYRQRAGSSKIAGTWRGSALAVRDISWCLLRLRLFGFRA"
BASE COUNT          735 a         1892 c         1768 g          791 t
ORIGIN      
        1 gcaaccgcta ctcctcgcgg ggcctcctgc tgtggaaccg gtacgaccag cggttcgccg
       61 ccggctacac gctgcgcgag gccctggacg cgtacatcgc gcgcatgctc cacacgcgca
      121 cgtcggtcgc gccggacacg cgggccaggg agtacttcga gaagaccatc gcgctgctca
      181 acgagcacgg gaccacgccg gtcatcgtga tcatgcccat ccatccgcgc gtgctgcgcg
      241 tcatgaagga gcacgacatg ggcggcgagc ggcaacaact gcgcgactat ctcgccgccc
      301 tcggagagac ggcgagcatc aaggtcctcg acttcacgac gatccgcagc ttcaacggcg
      361 aagccggctg gttctacgac ggcgtgcaca tcaaccgccg caacaccaac cgtgtgatca
      421 ccgcggtcaa ggccaaggcg ggcgagtacc tcaagtagag gcgccgtatg gcggcagggc
      481 agggaccgat gcagaagaag gctccggcgg cggggggtga tgcggccggc ggcggcgcgg
      541 ccgcgatccg gcgcgaggtg gtcgcgcttc tcggccaggt gctcgcgcac gacctcacgc
      601 agctctcggc cgcactcacg tactacaccg tgctctccct gctgccggcg ctcatcgtga
      661 tcgtgtccct gctcgggatg gtcggactgc agccggacac cgtgcacgcg ctcctcgaca
      721 cggtcggtga gctcggggcg ccgtgggccg ccgagttcgt ctccaccacg ctcgagtccg
      781 tcctgcgctc ccagaactcc ggcgtcgtct tcggcatcag cctcctcttc tcgctctggg
      841 cggcttcggc ctacgtgggc tccttcatgg cggcgtcgga ccggatctac gagatcgtgc
      901 gccggcggcc gttctggacg ggcatcccgc tgcgcctcgg gctcgccctc gcgctgctgg
      961 tcctgctctc ggtgactgcg gccgccgtcg ccctcgtcgg gccgttcggc cagtgggtcg
     1021 cggacgccac cgggatcggc cgcgggccgc tccacctgtg gacctgggcc aagtggccgt
     1081 tgctgatcgc cctcggtctg ctcctcttca cgctgctgta caagttcacg ccgagccgcg
     1141 ggcagccggc gctgtggtgg ctgctcccgg gcgccgccgt cggtatcgcc ctgtggatcg
     1201 gcgcgtccgc cctcttcagc ctctacctcg acaacttcgc ctcgtacaac cgcgtgtacg
     1261 gcaccctcgg cgcagcggtg gcgttcctcg tgtgggcctg gctgctcaac atcgccctgc
     1321 tcgtcggggt cgaggtgaac cgcgaggtcg agtcgcgaag gccgggacgc tccggcgccg
     1381 aggacggcgc ggggcgagcc gcggcgagcg tataatcggg gccccgccaa cgacgggccc
     1441 gcccgcccgc agaccgaccg ccggtgaccc atgcccgagc tgaacgcgct ccagaaagaa
     1501 ctgcgccgcc tcgccgccga gcgtgacgtg gtcatcctcg ctcacaacta ccagcgtccc
     1561 gaggtgcagg acgccgccga ccacaccggc gactccctcg aactctcgcg catcgccgcc
     1621 aaggacccgc accaggtgat cctgttctgc ggggtgcact tcatggccga gaccgcctac
     1681 atcctcgcgc cgcagaagac cgtgctgatg cccgacaagc gcgccggctg cccgatggcc
     1741 gacatggtca ccgtcgccgg gctgcggaaa ctcaaggccg agcacccgga cgcggtcgtc
     1801 gtcgcctacg tcaacacgtc ggcggacatc aaggccgaga ccgatgtctg ctgcacgagc
     1861 gccaacgcgg tgcagatcgt ggagcggttc ccaaaggagc agaagatcat cttcgtcccg
     1921 gacaggaacc tgggcgacta cgtggcccgg cagaccggtc gcgaagcgac gatgatcctg
     1981 tggcaaggct ggtgccacgt ccacgacgac ttccgcgccg ccgagatcga ggagatccgc
     2041 gccgccaacc cgggcagcgt gctcatggca catccggaga gccggcgcga ggtgctcgac
     2101 atcgccgacg tggtcacgag cacgtcgggg atgctccgct atccgcagac gagcgacgcc
     2161 cggacgttca tcgtggccac cgagacgggg ctgctctacc ggctcggcgt gctgtacccg
     2221 gacaggacgt tcctgcccgc ctcgaaccag gcggtctgcc cgaacatgaa gcggacgacg
     2281 ctcgagaagt gcgtcgcgac gctgagtgac gagtgggacg acgtgctcga gcacgaggtc
     2341 gtcgtccccg agcccatccg cgtcaaggcc ctccaggccg tggagcggat gctggcctag
     2401 cgggaggcgc ctgcttcagg tcagatgccg gtcgacccct ccgccgccgg ctctggctcc
     2461 gcgaacagca ccgtcgagag ataccgctca ccggtgtcgc acagcacggt gacgatcagc
     2521 ttgccccggt tctccggccg gctgccgacc aggtacgcgg cgtaggcgtt ggcgccggcc
     2581 gagatgccga cgaggatgcc ctccgcggcc agctcgcggg ccgcctcgaa ggcctgctcg
     2641 ttggtcacct gcacgacctc gtcgtagacc tgcgtgttca gcaccttggg gacgaacccg
     2701 gcccccatgc cctggatctt gtgcgggccg ggctggccgc cgctgagtac gggcgagtcc
     2761 gcaggctcca cggcgacgat cctcacgtcg ggcttgcgct ctctgaggac ctctccgacg
     2821 ccggtcacgg tgccgccggt gccgacgccg gccacgaaga cgtccacctc accgtcggtg
     2881 tcgccccaga cctcctcggc ggtggtgcgc cggtggacct cgggattcgc cgggttctcg
     2941 aactgctggg gaacgaacgc gccctcgatc tcccggccca gctcgtccgc gcgcgcgatc
     3001 gcgcccttca tgccctgcgc acccggcgtg aggacgagct cggcgccgta cgcgcgcagc
     3061 agattgcgcc gctccacgct catggtctcg ggcatggtga gcaccgtccg gtagccgcgt
     3121 cccgcggcga ccatggcgag cgcgatcccc gtgttgccgc tcgtcggctc gacgatcgtc
     3181 gtcctgtcgg gcgagatgag gccggcctcc tcggcggcgt cgatcatcga caggccgatc
     3241 cggtccttga cgctgccgcc ggggttgaac gactcgagct tgccgacgat cgtcgcgccg
     3301 gaccgctccg aaagggcgcg caacctgacg agcggagtgc cgccgatcag cgccgtgatg
     3361 tcttctgcga tcttcactgg tgtgctcctc tcgtggccat gctcagatct catacgtggg
     3421 cccgccggcc cccagctgca gctccctctc ggcgaggtcg ctgacggtcg tgcgcgccag
     3481 cacgtcctga tacgcatcac ccgcggcacg ccagacggcg ccggcggtgg ctcgctcccg
     3541 tgggtcggac atctcccgcg cggacggctc gaagtcgggt gcaccgtcca gcgcctccac
     3601 gaccgccagg acgctgaccc tgtcggggcg gcgcgcgaag gtgaaccccc caccaacgcc
     3661 gcggtgaccg gtcaggacac cggcgcgtcg cagggcggcg aagagctgct cgaggtactg
     3721 ctccgggatc cggcgctccg cggcgaggtc cgtgagacgc acgggcttct gctcgccgcg
     3781 agtgcgcagg tcgagctcga cgagcgccat gagggcgtac ctgctcttgg atgagacgtt
     3841 cacctgactc ctcggacggg gcgctcggcg gcgcccatcg tcgattctct ctcttcccga
     3901 tatgttttgt cgagaatcat cgtcgagccg gcggccgcac caggcagacg tggcataatc
     3961 gcatccgcac ccgccaccag gaggacacgt gcccgcgatc accgtcagcc tgccggccgc
     4021 gctcacggcc ccgcagcccg cccgcgacgt cgcctgcgag gcgtcgactg tggacgaggc
     4081 cctgcgcgcg gtcgtggagc aggcgccgca gtacgcgcag cgcatcttct acggcgaccg
     4141 tctgctggtc gtcgtgacgc tcaacggcag gcacctgccg ccgtcgacgg tccgggagac
     4201 cgagctgacc accggcgacc gcgtcgaggt cctgctgccg gtcgccggcg gctgaccgcc
     4261 ggcggctgga ccctccgggc cccggcggat gccgtcgtcc ggggcccgtc gcctgcccgg
     4321 tgcgtcgcct ggcggtcggc tgggtaccct ccgtctgtcg accgaggagg gcaacaccgt
     4381 gagactgtgg gcgacgcgcg agacgcgcag cgagttgatg ctggccgcgc tgcgcgtcct
     4441 gatgggcgtc atctttctcg ccgtctgggc ggacaacctc atcaaggggt actactcgcc
     4501 gagcggctgg gcggacttcg tgcagcagta cgccgacacc accaaggtcg gcctctacgc
     4561 ggacctgctg aactcggtga tgatccccaa cgcgaccgtg ttcgcctacg ggcagttcgc
     4621 catcgagttc gtcgtctgcg ggctgttcct cgtgctcggc ctgttcacgc cggtgtcggc
     4681 gctgctcggt gctgccttcc agctcaacct gctgatcgcg acgtcgggca ccggcgagtg
     4741 gccgggcacc tacatcatca tgtgcctgct gctggtcgcc gtcggcctcg cgcagtcggg
     4801 gcggacgctg gggctggacg cgctgctggc ccggagggac ccgcacccgc ggctgccggt
     4861 ctactgacgc gcggcgtccg ggccgggcgt cgccggcttc ccggtcaggc ccggaacccg
     4921 aacaggcgca ggcgcagcag gcaccaggag atgtcccgaa cggcgagcgc cgagccgcgc
     4981 caggtgccgg cgatcttgga cgacccagcc cgctgccggt agcgcgtgtc gcggacggcg
     5041 atgcgcgcgc cggagaggcg cgccttcacc gccatctccg tcgggaaggc gtacgcccgc
     5101 tcctccagcc gcagccgctc cagcaggtcg gtgcggacgg cgcggaacgg tccgtcatcg
     5161 ctgagccgca ggccgaagag cagccg
//
LOCUS       DASUMX010000187         4559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_66657, whole genome shotgun sequence.
ACCESSION   DASUMX010000187 DASUMX010000000
VERSION     DASUMX010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4559
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66657"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(252..1031)
                     /locus_tag="VFZ86_09290"
     CDS             complement(252..1031)
                     /locus_tag="VFZ86_09290"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5642516.1"
                     /translation="MRTSEVAEQVYWLPLGAGLRAANAYFIGSAGSWCLVDAGWPRDA
                     PAIRQAADELFGASARPAAVLLTHDHPDHAGAARELALAWGCQVWMHPLEVLLASADA
                     EAIRRYAGPLDRWVILPLLRLMGRRRMQAILAKGSLAEVAAPLDADAPVPGLPEWEVF
                     PTPGHTPGHVAYVRRDDRVVLTGDALVSVDLNSLRGIVSQERRLAEPPWYTTWSWPAA
                     KASAAAVAGLAPRVVAAGHGAPLTGPDAGTMLRAFLCASRR"
     gene            1262..2203
                     /locus_tag="VFZ86_09295"
     CDS             1262..2203
                     /locus_tag="VFZ86_09295"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX5642517.1"
                     /translation="MGAGPDLDRPPVIAMIGMATVDYLYVLDDHPQADSVNRALDYQT
                     AVGGSAGRGAIAARRLGGETRLLAACGTGVHAEVLRTELAAEGVACTWVAYDQPSQHS
                     AVIVAREAATRMIVWLPQPMADARMIAHLPAFLEGVDVALLDATDEALATAALHECEK
                     RAITTVIDTGSGRPWTRSLLGRVDHVIAPETFVQKETGHSAERAVVELWDDSCRVVFA
                     VTQGPRGGVFTTGREHERLQRWEAVPVAGVDSCGAGDTFHGAYAWAVARGLSPAECFA
                     AASWSAGLKVSRLGNEGIPTLEQLENARASSGVGKPV"
     gene            2270..3337
                     /locus_tag="VFZ86_09300"
     CDS             2270..3337
                     /locus_tag="VFZ86_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018779944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase"
                     /protein_id="HEX5642518.1"
                     /translation="MSGPQPTDGPLTPEELQLATRNRGMPLEALRHDVTPSGMHYLLV
                     HFDIPDVDPATWRLEIAGNVAAPRTLGLRDLRELPSRSLPVTMECAGNGRARLSPRPV
                     SQPWLVEAVGTAIWTGVPLRDVLAPAGVRPGTAELVFTGADHGVQGGAEQDYERSLTV
                     RDALRPEVLLAYEMNGRPLEPQHGAPVRLLVPGWYGMTSVKWLTRITAVTEPFRGYQQ
                     AVAYRYQRDEEDPGEGVSRMRVRALMIPPGHPDFATRRRFVRAGEVELRGRAWSGSGA
                     VERVEVGVDGVWADATLEPPVGEFAWRGWSFRWPAAPGEHVLSCRARDAAGQIQPLEQ
                     SWNFQGMGNNLVQTVPVTVQA"
     gene            complement(3378..>4559)
                     /locus_tag="VFZ86_09305"
     CDS             complement(3378..>4559)
                     /locus_tag="VFZ86_09305"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEX5642519.1"
                     /translation="LRIRGILRDPRSQDRYLATAQIGITIASLGLGMYGEEVVAGWLE
                     APFEQFGALGTAAAHGLASIVAVLLMTYVHIVVGEMVPKSIALQRAEHTAVLVSPVMR
                     VIWIVFFPLVMLLNGIGNAILRIFGVERQRGSVEQYHTAEELGYIVRESLEGGRLGSE
                     SGRVLQELLDFGDLTAGEVMVPRVHVVGIPIDASREEIVELLVSSPHTRYPVYREDLD
                     DVVGILHVKDVLRVLTQDTESEGYPVRPVPYVPETARVDVVLAAMRRENTQLAVILDE
                     HGGTAGLVSIEDLFEEVVGEIEEEAEQPDSWIDEADRLHVVGTVRIEEVGEHFEVELE
                     HEDVDTVSGLVLMLLDRPPEVGDRVTYDGVVFDVLAVEGHGVAECAVTALPPEAGDSA
                     E"
BASE COUNT          644 a         1600 c         1687 g          628 t
ORIGIN      
        1 tcccactggg tcgcgacgac ggtcatgtgc cggcgcggct ctccgctgtc ggcgctctcg
       61 gcggcgtcga ggaccgcctg gacggccgcc gccgtcccgg ggtcgggatg atgcagcctg
      121 tagcccgccg gcagtggggt ctggggcact ggtcctcctg acgaagtgag gctcatcgag
      181 tgtacccgcg tgcggacggc ggcaggcgca ccggcgcccg atcgaccagg ggcggcacgg
      241 cgcacgcggc gtcagcgtcg cgaggcacag aggaacgcgc gcagcatcgt gccggcgtcc
      301 ggacccgtca gcggcgcgcc gtggccggca gcgacgacgc gcggcgcgag cccggcgacg
      361 gcggcggccg aggctttcgc ggccggccag ctccaggtgg tgtaccacgg cggctcggca
      421 agcctgcgct cctggctgac gatgccgcgt agtgagttga ggtcgacgct cacgagcgcg
      481 tcgcccgtga gcacgacgcg gtcgtccctg cgcacgtacg cgacgtgccc gggcgtgtgc
      541 cccggtgtgg gaaacacctc ccactcaggg aggccgggca cgggtgcatc cgcgtcgagg
      601 ggcgccgcca cttccgcgag gctgcccttg gcgaggatcg cctgcatccg ccgcctcccc
      661 atcaggcgta ggagcggcag gatgacccac ctgtcgagtg ggccggcata gcggcggatg
      721 gcctccgcgt ccgcgctggc gaggagcacc tccagcgggt gcatccacac ttggcatccc
      781 cacgccagcg cgagctcgcg ggcggcgccc gcgtggtcgg ggtggtcgtg ggtgagcagc
      841 acggcggcgg ggcgggcact cgcgccgaac agctcgtccg ccgcctgtcg gatcgccggc
      901 gcgtcgcgcg gccagccggc gtcgacgagg caccaggagc cggccgagcc gatgaagtac
      961 gcgttggctg cccgcagccc tgcgccgagc ggcagccagt acacctgctc tgccacctcg
     1021 ctggtccgca tgccgtccgc ccgttggccg tctttggatg ccgcccgcac gttgacacta
     1081 ccagcagact cgagcgccgg gccgtttcgg gggcggcggg ccgccgatcg cagccgccgg
     1141 acagtacgtt cgttttgcct cgcctcgcgc ctgcccatat cctgaccagg cgcggcggcg
     1201 tccgcgggag cggatccccc gacaagacga gcgcgccaaa ggcgagcgca gaggaggccc
     1261 agtgggtgcc ggaccagacc tggaccgacc gccggtcatc gcgatgatcg gcatggcgac
     1321 ggtggactac ctctacgtcc tcgacgacca cccacaggcg gactcggtga accgagcgct
     1381 cgactaccag acggcggtgg ggggatcggc cggccggggg gcgatcgcgg cgaggaggct
     1441 cggcggcgag acgaggcttc tggccgcgtg tgggacgggc gtgcacgccg aggtcctgag
     1501 gacggagttg gccgccgagg gcgtcgcctg cacgtgggtc gcctacgacc agccctcgca
     1561 gcactcggcg gtcatcgtcg cgcgggaagc cgcgacccgg atgatcgtct ggctgccgca
     1621 gccgatggcg gacgcacgga tgatcgcgca tctgccggcg ttcctcgaag gcgtcgacgt
     1681 cgcgctcctg gacgcgaccg acgaagccct ggcgacggcg gcgctccacg aatgcgagaa
     1741 gagagcgatc accaccgtca tcgacaccgg gtccgggcgc ccctggacaa ggagcctgct
     1801 cggccgggtc gaccacgtga tcgcccccga gaccttcgtg cagaaggaga ccgggcactc
     1861 tgcggagagg gcggtcgtcg agctctggga cgactcctgc cgcgtggtgt tcgccgtgac
     1921 gcaggggccg aggggcgggg tgttcacgac gggcagggag cacgagcgcc tgcagcggtg
     1981 ggaggccgtg ccggtcgccg gagtggattc ctgcggggcc ggcgacacct ttcacggcgc
     2041 ctacgcctgg gcggtggcgc ggggactctc gcccgccgag tgcttcgccg ccgcctcctg
     2101 gtccgcgggg ctcaaggtgt ctcggctcgg taacgagggc atccccacgc tggagcagct
     2161 cgagaacgcg cgcgcgtcga gcggcgtggg caagcccgtg tgagcgacgg cggcgggccc
     2221 gggcaaccgt ccgccgtttg acccgtctga gatcggcaac gctgcaggta tgagcggtcc
     2281 acagccgaca gacggtcctc tcacccccga ggaactgcag ctcgccacgc gcaaccgcgg
     2341 catgccgctc gaggcgcttc gccacgacgt gacgccgtcc gggatgcact accttctggt
     2401 gcacttcgac atccccgacg tcgatccggc gacgtggcgg ctggagatcg ccggcaacgt
     2461 cgctgcaccg cgaacgctgg gcctgcgaga cctgcgcgag ctgccgtcgc ggagcctgcc
     2521 ggtgacgatg gagtgcgccg gcaacggccg cgcgcggctc tcgccacggc ctgtgagcca
     2581 gccgtggctg gtcgaggccg tggggaccgc gatctggacc ggtgtgcccc tccgcgacgt
     2641 gctcgcgccg gccggcgttc ggcccggcac cgcggagctg gtgttcacgg gcgccgacca
     2701 cggcgtccag ggcggagcgg agcaggacta cgagcgcagt ctcaccgtcc gggacgcgct
     2761 gcgtcccgag gtgctgctcg cgtacgagat gaacggccgg ccgctggagc cccagcacgg
     2821 agcccccgtg cgcctgctgg tcccgggctg gtacggcatg accagcgtga agtggctcac
     2881 gcggatcacc gccgtcaccg agccgttccg cggctaccag caggccgtcg cctatcggta
     2941 ccagcgcgac gaggaggacc cgggcgaggg cgtgagcagg atgagggtgc gtgcgctcat
     3001 gatcccgccc gggcaccccg acttcgcaac gcggcggcgc ttcgtccgcg cgggtgaggt
     3061 ggagctgcgc gggcgggcct ggagcggctc cggcgccgtg gagcgcgtcg aggtgggcgt
     3121 cgacggcgtc tgggcggacg ctacactcga gccgccggtc ggcgagttcg catggcgcgg
     3181 gtggagcttt cgctggccgg ccgcgcccgg ggagcacgtg ctctcgtgcc gggcgcgaga
     3241 cgcggcaggg cagatccagc cgctcgagca gtcctggaac ttccagggca tgggcaacaa
     3301 cctggtgcag accgtgccgg tgaccgtgca ggcgtgacgc aggacgagcg gaccgccggc
     3361 gagcacgacg gccggtctca ctcggcagag tcgccggcct ccggcgggag tgcggtcacg
     3421 gcgcactccg cgaccccgtg gccctcgacc gccagcacgt cgaagacgac gccgtcgtac
     3481 gtcacgcggt cgccgacctc gggagggcgg tccagcagca tgagcacgag gccgctcacc
     3541 gtgtcgacgt cctcgtgctc gagctcgacc tcgaagtgct cgccgacctc ctcgatgcgg
     3601 accgtgccga ccacgtgcag gcggtcggct tcgtcgatcc acgaatcggg ctgctccgcc
     3661 tcctcctcga tctcgccgac gacctcttcg aagaggtcct cgatgctcac cagcccggcg
     3721 gtgccgccgt gctcgtcgag gatgacggcg agctgggtgt tctcgcgccg catggccgcg
     3781 agcaccacat cgacgcgcgc ggtctcgggc acgtagggca cgggacgcac cgggtacccc
     3841 tcagattcgg tgtcctgcgt gagcacgcgc aagacgtcct tgacgtggag gatgccgacg
     3901 acgtcgtcca ggtcttcgcg gtacacgggg tagcgggtgt gtggtgagga gaccagcagc
     3961 tcgacgatct cctctctgct cgcgtcgatg gggatcccga cgacgtgcac gcgcggcacc
     4021 atcacctcgc cggccgtgag gtcgccgaag tcgagcagct cctgcagcac tctgcccgac
     4081 tcgctgccca gccgtccgcc ctccaggctc tcgcgcacga tgtagccgag ctcttcggcg
     4141 gtgtggtact gctcgacgct gccgcgttgc cgctctacgc cgaagatccg gaggatggcg
     4201 ttgccgatgc cgttgaggag catgaccagg ggaaagaaga cgatccagat gacgcgcatc
     4261 accgggctca cgagcaccgc ggtgtgctcg gcgcgctgca gggcgatcga cttcggcacc
     4321 atctccccga cgacgatgtg gacgtacgtc atgagcagga cggcgacgat gctggccaga
     4381 ccgtgggccg ccgccgtccc aagcgctccg aactgctcga agggcgcttc gagccagccc
     4441 gcgacgacct cctccccgta catcccgagc cccagactgg cgatggtgat gccgatctgg
     4501 gcggtcgcca ggtacctgtc ctggctgcgt gggtcgcgca ggatcccacg gatccgcag
//
LOCUS       DASUMX010000188        35156 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1071451, whole genome shotgun sequence.
ACCESSION   DASUMX010000188 DASUMX010000000
VERSION     DASUMX010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 35156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35156
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1071451"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..105
                     /locus_tag="VFZ86_09310"
     CDS             <1..105
                     /locus_tag="VFZ86_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008824688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX5642520.1"
                     /translation="ISGEKKQLHVIDAEDCIKCGICRDVCRFDAVLVQ"
     gene            115..879
                     /locus_tag="VFZ86_09315"
     CDS             115..879
                     /locus_tag="VFZ86_09315"
                     /inference="COORDINATES: protein motif:HMM:NF024900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX5642521.1"
                     /translation="MDAELIIDGQTVTAAEGSSLLSAARAAGRDVPGLCHHEAVAPIA
                     SCRLCLVEVQRPGRDHVHLTTSCDYPVSAGLVVVTDSARIRTHRTMNLELLLRRAPEA
                     PVLLALAADLGVTTPRFAPLADASLPDCILCELCVRVCSALGYNALAAIGRGEAKSVG
                     PPFGLAEAPDCVGCGSCVEECPTDCIPMVDTPTTRTIWGRTFALKACVRCGRPITTRE
                     HAAAMDAAWQLPEKDEGLCAVCKKRVVSERLAAPGR"
     gene            complement(1245..1625)
                     /locus_tag="VFZ86_09320"
     CDS             complement(1245..1625)
                     /locus_tag="VFZ86_09320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642522.1"
                     /translation="MKRALLPLATGCVLVGVVAIVLAVVGWSWGWFVLANGALAVLLL
                     MLFRGSVTGADPDRPPGVARVSAARPVGPFDPGTLGAGESALEPDPDVAAEARAEDVA
                     TGFGFLWTAVAPAGAAVLAALMYL"
     gene            complement(1622..2548)
                     /locus_tag="VFZ86_09325"
     CDS             complement(1622..2548)
                     /locus_tag="VFZ86_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017299529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX5642523.1"
                     /translation="MAAESKKAVVAAIIGNLAIAVVKFIAAAMTGSSAMISEGIHSLV
                     DTGNGGLLFYGLKRGARPADEQHPFGHGMEVYFWSLIVAVSIFGIGGGMSIYEGITHI
                     QHPVALQDPTINYIVLAVAMLFESYSFSVAWRIFRKSKGRRRTLAAIHHGKDPSLFTV
                     LFEDTAALLGLAVAFVGIFLSHQLEEPVIDGLTSVVIGGILVVAALWLAYESRSLLVG
                     EAADPEMVAEIRRLILADDAVADVGPVLTMHLGPDDVLLNIEAVFSPGLSAEEIHDAV
                     HRVEGRISGPYPEVSRIFIEVEALTGPRKGEP"
     gene            2657..2875
                     /locus_tag="VFZ86_09330"
     CDS             2657..2875
                     /locus_tag="VFZ86_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642524.1"
                     /translation="MAGSSEYECGECRHRFRAPEGVPRDARTLMCPACGSIDLNLMSV
                     ERPRPVVMVPRAGTPSPLWREPRETKAS"
     gene            2957..3691
                     /locus_tag="VFZ86_09335"
     CDS             2957..3691
                     /locus_tag="VFZ86_09335"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX5642525.1"
                     /translation="MLRAVTFDFWSTLFVDSRGREREALRARVLADTLCAAGLQASGY
                     ALDESLRASWDHFDRVWAREHRTPPCAELVDAILGSLGVTVPDDLLVPLVDRFERLVL
                     EVPPEPMPGAVYTMPQLAERYRLAIICDTGYSPGAVLRQLLDRHDLLGHFEYAYFSNE
                     HGMSKPDPRVFVHVLEELDVRPAEAAHVGDIQRTDVAGAQAAGMSAVHFVGANNADVP
                     HSTGDAIVSHFEELPQALGSLICAGC"
     gene            3678..5039
                     /locus_tag="VFZ86_09340"
     CDS             3678..5039
                     /locus_tag="VFZ86_09340"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX5642526.1"
                     /translation="MPAAERPRLLGRQRRPYLIAALAGLAVFVLVTILMRTGALDGLD
                     TRVVTDFAGGRSQPLTGIAETLDALGAWWLLLLLVAVLVGGLWWSGRMVQALYLAASV
                     AAAVIVNPVLKQVFARTRPEDDQLVTVSSTAFPSGHTTASATIATALAVIAWPTRWRW
                     PVIAAAAVFSLAMAVSRVYLGVHWPSDVVGGLALGFTVAMTVRLLMPWPSPEEAEAAR
                     QAAGAAAPAASDLPGIDVVFLDWGNTLMVDSGMREGPMKDWDTVEAEAGAKEALLRLR
                     GRYRLVVATNAEDSPAPDVRFALARVGLDECVDEVITSADVGDHKPNYAFYRAALLQE
                     GVRGLPLDPRRAVMVGDGTTNDIAGAQRAGMRTIWYNPTKRRFPDGAPPPDAVIRKLA
                     DLPAAVDKLAGVAPEKRSRKRSEAETAAAAAAAAAAAKDLRAETAAEDSAASAAAAIP
                     DGR"
     gene            5042..5581
                     /locus_tag="VFZ86_09345"
     CDS             5042..5581
                     /locus_tag="VFZ86_09345"
                     /inference="COORDINATES: protein motif:HMM:NF021561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2284 domain-containing protein"
                     /protein_id="HEX5642527.1"
                     /translation="MDLSHPEQWLRRALQLGVDAATPIDADAVVAAEWVRMKCLYGCD
                     EPGIRKTCPPNLPPVAVTQRLLSEYRRGVLLEVGPIVGREASDPESRRLNDAALALER
                     DLFLAGHHKAWMMGAGPCDLCDACGTGRECPTPEKARPSLEGCGVDVFATVRNAGRTI
                     DVAWDEDDEYRFFALVLVE"
     gene            complement(5587..6462)
                     /locus_tag="VFZ86_09350"
     CDS             complement(5587..6462)
                     /locus_tag="VFZ86_09350"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA family NAD(P)-binding protein"
                     /protein_id="HEX5642528.1"
                     /translation="MKTTSGPVYVVGAASHQGRATLDRLGAFDVEARALFDERDARRH
                     APRWDVPGTRTARFDDPSALDRTLRGAGALVLILEDPDTGPAERLRHGRSMIEAASRA
                     GVGHIVFTAATGPDHHRLSCDVSAETERYLVRQGVPATVLRPATFMEEVPWYWLSRFG
                     RELTLATPFGADNHLPLVALDDVGALVALAVTHGEEFVGRTIDVAGDTRTPLDIAARL
                     SEALGEPVRYEEVQVEGVFVYQEASTPVHDIEWLRRVHPQLHTFSSWLETGGGLELCR
                     HHLGLAASPETRQAA"
     gene            complement(6591..8144)
                     /gene="gltX"
                     /locus_tag="VFZ86_09355"
     CDS             complement(6591..8144)
                     /gene="gltX"
                     /locus_tag="VFZ86_09355"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEX5642529.1"
                     /translation="MSEHSHIRVRFAPSPTGYLHLGSARTGLYNYIFARQHEGTFILR
                     IEDTDLSRSTDAAIVQIIASMKEMGLHWDEGPGVPGPHGPYRQTERDGIYHEYVETLL
                     ESGHLYPCFCTPGELEAERTAAQADGRGWVYSGRCRALAPEDVERRRAAGEPHTLRVR
                     VPAGRTVFHDILRGEVAFENETMGDFIVQRSDGTVTYNLAVVVDDVTMEITHVIRGDD
                     HISNTPKQVVIYEALGAGVPEFLHMPLLFGTDKKKLSKRHGATKLEQLTAEGFLVEVV
                     RNYLCFLSAEFDETMITWSLDDLVRDFDVTRLGTSPSIFDPEKLRWMNGRYLRAMTDE
                     WLGGRLVEYLTRVGFYGDPAGVGELAAHGPQALAAAVEGAETEHLPPTVPDEEQEALT
                     REVAPLVHEKLEVLADFVPMAGWFFRPLAFAPEALERLRGTGGAAETLAAAAAGLRDL
                     EPWDVPAIESLVRDLPATLELKPKAVFAALRLGMSGQSVTPGLFESLWVLGRDQAVAR
                     LEQTAALLV"
     gene            8311..8595
                     /locus_tag="VFZ86_09360"
     CDS             8311..8595
                     /locus_tag="VFZ86_09360"
                     /inference="COORDINATES: protein motif:HMM:NF019278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEX5642530.1"
                     /translation="MVGVGHRNQQFPELRWCTPERGYSGWMAESYFEYTSPTEAIVRK
                     EYDASQLTVLEGEELDVLDVVGEWWLCRNDRGIKGWVPQRILEDATPGDS"
     gene            8607..8978
                     /locus_tag="VFZ86_09365"
     CDS             8607..8978
                     /locus_tag="VFZ86_09365"
                     /inference="COORDINATES: protein motif:HMM:NF019278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEX5642531.1"
                     /translation="MITDSKSRQVRVVTSCTREQVQEIRMRKGDSLAVGHRNQQHPAF
                     IWSATEDGHHGWVPEDYVEVVGGRDAVARRDYDSTHLTVSSGERLEAIEQVGDYLLCR
                     NAAGVEGWVPESCVEETVEEA"
     gene            8975..9355
                     /locus_tag="VFZ86_09370"
     CDS             8975..9355
                     /locus_tag="VFZ86_09370"
                     /inference="COORDINATES: protein motif:HMM:NF019278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEX5642532.1"
                     /translation="MTEAQLPRRVRVIEPHVTNDPNPIRFRAGDTLGVGHHDQVWKRY
                     VWGTDQAGRSGWVPDTYLEFGADGHTAIALRDYDSTELSVGRKQILEVLDEAGGWYLC
                     RAESGSSGWVPGTSVEPVEPAEPT"
     gene            complement(9348..11105)
                     /locus_tag="VFZ86_09375"
     CDS             complement(9348..11105)
                     /locus_tag="VFZ86_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642533.1"
                     /translation="MTRGRQSRRVAPGLVALLACALLASVLLAAGLGLPAAASAAGSV
                     DPTAPGPVKPVPPVLDDLMGRGDGVAAPALPIGLGALPGARETAAFAAGRVAVGVVFL
                     ESDGSIMTSSEDWTRQDPDYPGRDRRQLVLAMIQGALDWWNARSPDGSLELFLPAAGS
                     YGAPQTVPTGYEPIRRDVAVSWKGRPVLSDAAWRWQAMGRLGFAHDATDDTPYPETLF
                     ADRLRRRNGADWAFVVYVVDSLQDEDGMFRNRVVAYTADLFGPYCTLAYDNDGYHFAS
                     FDAVLAHEMGHVFGALDEYAPPVPGYPSTGDLRSGYLGVRNGNAVRGGTTDLPCIMGG
                     SNATLAAFRDGDLCRWTAGQTGLRDSDADTRPDVVDTRPGISTRLESTDAGGVVTLRG
                     TVTEQPRPRGRISGGVYFRRDLSIKVPHEARYRVDDGAWQPLTATDGAFDEPSEGWTL
                     TTEPLTPGHHDLELEATTGETAGRTRDLWAGPTPVTLELATNAAFTPTAVTVAAGSAA
                     RVYVRTTSAGLPVARLTPLRLVRLVDGKVMARLTTGENGVWTGTLRPARTRAYEVRFA
                     GAGQFLGPVRSGRVTIRVR"
     gene            complement(11143..12594)
                     /locus_tag="VFZ86_09380"
     CDS             complement(11143..12594)
                     /locus_tag="VFZ86_09380"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX5642534.1"
                     /translation="MIIIPLIILTIAAIVIVVLSFVGIRGFRRGFRQGLREDAPEHAP
                     LRTTLFRTVLVTLLVLFLLVIVIVDRQQPREDQWPADEGLAKLDRFISRGEARWIDDE
                     AHAAIRQALLDATDAQGSGGGTVFLDGTMITDLTPATTARLEERGWDVGQGLVGPEDR
                     DWRYVAWRTSDERAVLYVYDPAPWWSHVVQYLLSLLIGLALLSPFAAVAAWYLNRRIV
                     KPVQQVAHASVVLADGGIPVAIPTKAPLELSVMAQSFNRMARKLKKAQDTERDFLMSV
                     GHELKTPLTAIDGYAELLQDGAVAPGEAAEVLSAESGRLRRLITDLLDLARIDRSEFT
                     VVDEPVDLADTAREAVARFEGLAGSLGVALVADAAEPAPARGDAGRLLQVASNLVENA
                     LRATSSGGRVEVVARPGRLSVRDTGPGLTEKDLDHAFERFYLHRKYSTGPTVGTGLGL
                     AIVKDLTEAMGGSVEAESEPGEGSTFTIDLATT"
     gene            complement(12591..13256)
                     /locus_tag="VFZ86_09385"
     CDS             complement(12591..13256)
                     /locus_tag="VFZ86_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013161723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5642535.1"
                     /translation="MTTVLVVEDEHNIATVVRIALERDGHRVVAVRTGEEALAELPRH
                     PVGLVVLDLGLPGMDGLEVCRRIRAGSSLPIIMLTARDEEADRVVGLELGADDYVPKP
                     FSPRELAARVKAVLRRSTASPRADATTVGDVSLSRSRHEVHIGDRPVQLSSTEFNLLA
                     FLMENAGLVFSRELLLERVWGLEFPGGTRTVDQHVAQVRAKLDLPGFIETVRGAGYKV
                     VSR"
     gene            13476..14423
                     /locus_tag="VFZ86_09390"
     CDS             13476..14423
                     /locus_tag="VFZ86_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEX5642536.1"
                     /translation="MPQLRIVVLVKQVPDTANVTGEAMKEDGTINRAALPAVFNPEDL
                     NALEEALKLKDRHDARVTVLTMGPPAAVQVLRESLYRGADEVILLTDKAFAGADTLAT
                     SYALSLAVQKLGGADLVLCGRQAIDGDTAQVGPQTAEKLGYPQITCVSRVDELRAPDG
                     EAGSGAGGEAGSITATRSIEGGFETLTSSLPCLLTVTGEANDPRPPSVKLVMTYKGIA
                     AEAMPEYDDSYLDPERDEAEVCMIGKDGKPVCRARVIQWDHAALAADREALGLRGSPT
                     RVKEIESVVLAQSEARMVAPDEAGITELVHELIEEHILG"
     gene            14416..15558
                     /locus_tag="VFZ86_09395"
     CDS             14416..15558
                     /locus_tag="VFZ86_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010527472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEX5642537.1"
                     /translation="MVDTANTNDVWVVVEQHDGEAAEVSLELLGRARELADQLGVQVG
                     AVLIGAGEAAKALAPALVARGADTVYFVAHTDLAHYLAQPYTQVVTGLIREHEPQIVL
                     YGATTTGRDLAPRVASALRTGLTADCTDLRIGDHALKGEEFKDLLYQIRPAFGGNIIA
                     TIISPLHRPQMATVREGVMVMPEEDRRRDGRIIAVPVTRARERIAVRAGVVLSDEDFV
                     VTLVKRVQEEKRVDLKGARIVVSGGVGVGSREGFALVEELARTVGGVVGASRAAVDAG
                     WIDHDHQVGQTGTTVRPKLYIACGISGSVQHRAGMDQSARILAINNDPLAPIFSVAHY
                     GVVGDLHKVIPLLIQAYKTKGAGGGAAPAPGQAAPAQPAAADGGAS"
     gene            15555..17309
                     /locus_tag="VFZ86_09400"
     CDS             15555..17309
                     /locus_tag="VFZ86_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019129347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5642538.1"
                     /translation="MSNYFGDNADLQQTLRRLDLARVVRLREDDYAQARDFAYAPADF
                     EDALDSYARTLEIVGDIAGEFVEPRAEDVDRAGAELIDGEVVYAPGITESLERLTQAE
                     LMGITLPRKYGGLNFPVSVSVMIVEMVSRADPALMNIFGLQDISETVNKFADDAMKAA
                     YLPRFASGEVTGSMALTEPEAGSDLQNVQLKATEQPDGTWRLNGVKRFITNGNGRISL
                     VLARSEEGTTDARGLSMFLYERDEHMRIRRLEDKLGIHGSPTCELQFDEAPALLVGER
                     RRGLTTYVMSLMNGARLAIAAQAQGIAEAAFRAAAKYAAERIQFGAPIGELTAVKGLL
                     ADMKVSIEAARALLYETALIVDTKEVLEHRIAQLQAAEKAGEAPPAGPDTDVKSLRAE
                     LKQYTRLAALYTPLAKACCTEMANQVAYDSLQVHGGSGYMRDFAVERHARDARITNIY
                     EGTTQLQVVAAIGGILGGTLAGRLDEYDAEDFSATPDLLARVRGARARLAEAIARVRD
                     TDDVRFRDFHARRLAEMAIDVDCAYLLLRPAQTDARRLLAAEYFVAAAAARVEGAAAQ
                     VLTGDPSTLDALTTLAAG"
     gene            complement(17351..18865)
                     /locus_tag="VFZ86_09405"
     CDS             complement(17351..18865)
                     /locus_tag="VFZ86_09405"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HEX5642539.1"
                     /translation="MDDHARPGAVAGRGVEDLLEAVVANVGEALDLWSVDLWAFSDDA
                     DTLECRAYWCRDPKTAKTRGCVGAVIGLDQSHDLRRLVLTGQLVERHVDDGLPPADAA
                     ALAQAGFTHRIDVPLLAGAEVLGVLSLAETRSGHRLSEEERGRLGELSRLAAAVLRAS
                     GLYEAERARTERLTAMLAAGRGISATQSPAAIVAAVREEAAGLVSGVVCDVQVMLRQD
                     DGTFAPAGEAGKERPAGGWRADALARQAADLGRPEQARTADGRARLLLPLTAGERAGG
                     YLEFTAALRRPFREAEIELASLLAGQAAAALERAKAFRAVQSRSATDVLTGLYSRWYF
                     YERLYAEVARARRYRQPLSLVVAELDGEEELARRRGASFRDAALAALARLLMTSLRDK
                     VDVACRLGGGRFALLLPNTPPGPDAAGLVAERVRQRVAATRLSNDELGALGSFTMSFG
                     VAGFPEDAEDADDLAGVAEDRLAAARAAGGDRVEPPPPEPEEDAEDEAPDDEPA"
     gene            complement(18858..20963)
                     /locus_tag="VFZ86_09410"
     CDS             complement(18858..20963)
                     /locus_tag="VFZ86_09410"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX5642540.1"
                     /translation="MTEIRDARERAARDPYRTVLEVATTIASSLDLDEVIQTIARQVG
                     EALDVQWCDINEYDPEARTMTYVAVWSRELRRVDVDYIGTVVSLGDRPERDAVIRRGD
                     LLEEYVDDDDLDPLEREVMIRYDEKAVMEMPLVFRGEPLGVLGVVESRRDRRFTDEER
                     QLLRLLAGPAATAIGNARLFRQQQEQERRLGTLLDASRTLTASVHLDEVLDGVARLAA
                     EAAGATYATVYEYRPHHDAIVYRAEHSPGRPPEGVHDDALGTVYALADLPGERVILGA
                     PEAVLEQISDPALPGDRRRSMESWRQKTCLSLPLRAAGAPQGILRLTMTDAERRFTAP
                     ELELLVALAESAGAAIHNARLFRAQEEESERLLELLEVSRRLTATFDRREVMTALQES
                     AAKLLAGGATAAVWLPEAGCPPAVTPRTLGAEAAELAQEALAAGCTVDRAGAERSGLA
                     VPLAIKDRVEGVLVVEADGHRRFVRGEREALQVLANQAAAALENERLYRRAEQEAIRD
                     GLTGLYNHRHFQERLRQECRRAQRYGTPLSLLMLDLDDFKEFNDEFGHQIGDEALREV
                     GQILFAVTRRGVDIAARYGGEEFAVILPHTRAADLPAQGASAAGGDDPEAPPPAGAGA
                     LIVAERVRAAIAGHAFPGHGGRRYAHTTATVGVADLRAGDDAASLIAAADAVLYEGKR
                     SGRDQVVARGADGRAETHG"
     gene            complement(20956..23043)
                     /locus_tag="VFZ86_09415"
     CDS             complement(20956..23043)
                     /locus_tag="VFZ86_09415"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HEX5642541.1"
                     /translation="MSSAAETEPFRLAMEAGAEITAALAGDDAPTVIVRRVAQALDAW
                     ECDLYEYDPETRTLTAAALWARELTDEDLAWVGTRISLDERPGYVPIIEGGACVLSAV
                     DDPDLDPADRTAMLAWGETATLAVPLHFRDRGVIGCLTLIEKREGRRFTDEDCELVVL
                     LAGPAAIAVCNARLFRLQQEQNRHLSSLLDTGRAITSAVTLEDSLTSICRTAAAALDT
                     EECIAYEYDAGRDAIVCRAFWGVHGAPPALDERGVYPLDDYPTDREILRRGEVVQETL
                     SDASLAGDVRASMEQWGEKACLNVPLIVEGERLGILVLIETERERTFGASEVELARAL
                     GEQAATSIRHARLYRRGEQQNRRLLTLLETSRVLVSSLDADAVLGQVRRDVADLFGVG
                     GDAVAMLLRRGEAFVPFEPGPVAAAAGEQAAVELDELHRRAVDERAVALSAEDGGSRV
                     VVPFVAGDVVEGLLDIVAASSSAFSDDELELVQILASQAAAALANAGLYRMLERQAIT
                     DGLTGLYNHRYVYERLNQEIARAQRYGLPLSLLMIDIDDFKSFNDRFGHPTGDLVLVE
                     VGRILGTHIRVGIDIAARYGGEEFVIVLPNTAREGAQVVGTRLAHQLSSLPGAPPPHE
                     EGALGVSERIRVSVAASELPGVDGDSRITVSIGVACYPGVAGGPGELVRNADKALYLA
                     KRLGKNRVEVFGD"
     gene            23444..23641
                     /locus_tag="VFZ86_09420"
     CDS             23444..23641
                     /locus_tag="VFZ86_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642542.1"
                     /translation="MIYCVVPPELGEDAYKRLVEHYRDNPNVKVIMDRRHGERRTDRS
                     GGGERQTRDRRRRRAPGSYDI"
     gene            23660..24142
                     /locus_tag="VFZ86_09425"
     CDS             23660..24142
                     /locus_tag="VFZ86_09425"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015751144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEX5642543.1"
                     /translation="MNEPLPRGSETSLVLTHLMQPADANYLGNIHGGIIMKLIDEAAG
                     ACAFRFCRTNIVTAAIDRIDFHHPVHIGNLVRLLANVNFTGRTSVEVGVRVEAEDLRT
                     GMVTHTNSAYLVFVALSGSGRPMPVPRLTPQTDEQRLWYEKAAERRRKRLAEREPADD
                     "
     gene            complement(24221..25018)
                     /locus_tag="VFZ86_09430"
     CDS             complement(24221..25018)
                     /locus_tag="VFZ86_09430"
                     /inference="COORDINATES: protein motif:HMM:NF018798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HEX5642544.1"
                     /translation="MTMQLGGMALKDGVLLQSDGYWAAAVREEGGGIRVVSGSKVRLP
                     GREALRRVPVVRGVARLGEAMAVLPAVRRATGTPVLPQEDPKLLAATAASAAVTVALR
                     ATRLGSPVLRELAVAGVSLAPVLVALRDSRLSRYHGAEHKSIAAYESGGDPAGADKEH
                     ARCGSNLVAPMVVTSLATNLVLRAAGRERQPLATFIAGLVSIGTAMELFSWMSRHQDH
                     PLARTLGRPGIEMQRLFTTSEPTTEQLDVADVALRELLRLEGEAPAA"
     gene            25206..25901
                     /locus_tag="VFZ86_09435"
     CDS             25206..25901
                     /locus_tag="VFZ86_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642545.1"
                     /translation="MGFFDTVRDKAGALATDAGRAGKVTAAQARLVVLQNDLRKAERE
                     LGHAAFALAERGELDHPDLAHAVERLRATAAEVSAKEAEIAALRDEPLAPAAASGEAP
                     APAATVAATTVQTAAGPVTVAAAEPEPVVAATPPAPEAAPEAPAAAPTEAGARPEAET
                     AVAEAAAKPARRRTAVKPPIPVAKKAPAKKAPPASKATARKTSSRAGATPKKSSRPRS
                     GPRPAGKNHPAGT"
     gene            25993..27003
                     /locus_tag="VFZ86_09440"
     CDS             25993..27003
                     /locus_tag="VFZ86_09440"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX5642546.1"
                     /translation="MTDQTCIPGTTDARIPICELKSGQAVEGPYCCSDKIAATDRNGK
                     AYLRLKLRDASGEVAAIHFDPSDDALGITVGDVVIVGGTFSVHPQYGAQVQVRRLRAA
                     DPDEYAACDLVPVSPIGPAELAERLHVLVDSVEEPHVRALLERALDGTREPGATFAVA
                     PAAVRNHHAYRHGLLEHSLIVAEAAAGVASRFASVDYDLVVAGGILHDIGKTLAYTSD
                     GMAPQMTDSGRLHGEIVMGHDMVRDLIDEDPDFPPDLALRLRHIIVSHHGMREKGSPV
                     VPQTREAVIVHYCDDMTARIGAIDDAEAAAPAGDRWTSRIFMIDAPAYVGAREEAADA
                     EE"
     gene            complement(27013..27321)
                     /locus_tag="VFZ86_09445"
                     /pseudo
     CDS             complement(27013..27321)
                     /locus_tag="VFZ86_09445"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
     gene            complement(27334..27462)
                     /locus_tag="VFZ86_09450"
     CDS             complement(27334..27462)
                     /locus_tag="VFZ86_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642547.1"
                     /translation="MLKEGDKAPAVTGVSYDGTGFDLGAPGGRTVLFFYPKANTGG"
     gene            27600..27812
                     /locus_tag="VFZ86_09455"
     CDS             27600..27812
                     /locus_tag="VFZ86_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005148064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEX5642548.1"
                     /translation="MARKLKVSVDYDRCTGVGACEQVCPEVFFMNDEGLPDVLEEQPH
                     ETLWAAVERAEEACPEEAVILEWTGE"
     gene            complement(27828..28463)
                     /locus_tag="VFZ86_09460"
     CDS             complement(27828..28463)
                     /locus_tag="VFZ86_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014346065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5642549.1"
                     /translation="MLENIHWLGHDSFRLDGTATVYIDPWKLPSGSPPAGLVLVTHDH
                     FDHLSLPDIEAIAGPDTVVVGPASVTGQVRGLETVTVAPGDTASVAGVEVLAVHAYNL
                     DKFRAPGQPYHPRESGGVGYVVELDGVRYYHAGDTDVVPEMADVRCDVAFLPVGGKFT
                     MTWEEAAEACDLIGPAVAVPMHYGEIIGDIRDAERFRDRSRVPVTILPLER"
     gene            complement(28794..28988)
                     /locus_tag="VFZ86_09465"
                     /pseudo
     CDS             complement(28794..28988)
                     /locus_tag="VFZ86_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003719639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
     gene            complement(29183..29782)
                     /gene="metW"
                     /locus_tag="VFZ86_09470"
     CDS             complement(29183..29782)
                     /gene="metW"
                     /locus_tag="VFZ86_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009615228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HEX5642550.1"
                     /translation="MQRRIDYKLIEDLVPEGATVLDLACGDGELLSELIAEKHVRGSG
                     VEIDQAAVEKCIGRGLSVFHGDLDAGLADFGDQSHDVVILSLSLQQLRRPRMIVREMV
                     RVGRLAIVSFPNFAHWTPRSQLLLRGRMPVSRDLPYQWWDTPNIHLCTVQDFRKLCRE
                     EGLRIEHEMYLRAVDKDPPARIVAPNLLARIGIFAVTRC"
     gene            complement(29794..30996)
                     /locus_tag="VFZ86_09475"
     CDS             complement(29794..30996)
                     /locus_tag="VFZ86_09475"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013163619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEX5642551.1"
                     /translation="MTESKPAARKEIGDVAMGYDDDAGNAGIVRTRRVTFDQPGQELA
                     LEGGGTLAPFTLAYETYGSLSERKDNAVLVTHALSGDAHVAGRHSANDKRPGWWDMMI
                     GPGKGLDTDKYFVICANVIGGCTGSTGPASTNPATGKPFGTDFPIITIADMVNAQVRL
                     LDHLGIDKLLAAVGGSMGGMQVLELVVSHPDRTHLCVPLATAARQPTQAIAFNEVGRQ
                     AIMADPNWRGGHYYGGKTPAKGLSVARMVGHITYLSDEAMEEKFGRRLQDILDYTFTF
                     SADFEVESYLRHQGLSFTNRFDANTYLYITRALDYFDLSRQHGTLVKAFEEVKARFLV
                     MGFSSDWLHPPYQLKEIVSALRATHKHVSYYEVESHYGHDAFLLEREKMEGIIAAFLS
                     SGARTYHR"
     gene            complement(31037..32332)
                     /locus_tag="VFZ86_09480"
     CDS             complement(31037..32332)
                     /locus_tag="VFZ86_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013074810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
                     /protein_id="HEX5642552.1"
                     /translation="MTDSDTKAQGFETLALHGGQEPDPATNARAVPIYQTTAYTFESS
                     DQAADLFSLRESGNIYTRMMNPTNDVFEKRVAALEGGVGALAFSSGSAAVTFSILNIA
                     GAGDHIVSANSLYGGTYNLFVHTLPRFGLDIDLVDPADPEDFRRAIKPTTKLLYAETV
                     GNPKLDTLDIEAVAAIAHEAGVPLIVDNTMATPYLIRPFDHGADIVVHSATKFIGGHG
                     TSIGGVVVDSGAFDWSQNDKFPGLVEPDASYHGLKFHEALGPITYIVKLRVSLLRDVG
                     AALSPFNAFLFLQGLETLPLRMERHSLNARGVAQYLQGHAKVAWVGYPGLECHATHGI
                     ACKYHYRGLFGAMVGFGVAGGREAGRRFVESTRLLSHVANIGDAKSLVIHPATTTHSQ
                     LTAAEQLECGVSDDFVRLSVGIETLDDIVADIDQALAKA"
     gene            complement(32348..32797)
                     /locus_tag="VFZ86_09485"
     CDS             complement(32348..32797)
                     /locus_tag="VFZ86_09485"
                     /inference="COORDINATES: protein motif:HMM:NF014173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX5642553.1"
                     /translation="MIHLSTKSDYAIKALVRLALADGTAPVTARDIVAFTGIPPKFLE
                     QVMHDLRQAGLVESQRGKGGGYVIARDPATVSFADVIDLIDGRRADPAARAGSNGGKA
                     RMRGGGRADALVAPVWREVRDCTRAILGAATIADAAARAAEAPMYYI"
     gene            32937..33872
                     /gene="murB"
                     /locus_tag="VFZ86_09490"
     CDS             32937..33872
                     /gene="murB"
                     /locus_tag="VFZ86_09490"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEX5642554.1"
                     /translation="MTPPSDQTQATADPRVREALRRELPGLEEAVPLASITAYGVGGP
                     AQFLVRAGTTGVLVHAVLTARELGLPVTVLGRCTNVLVGDAGVSGLTVLARNEGTTLH
                     GNVLRAEAGVELPGLVADLAGQSLAGLTFAANIPGSVGGAVVGNAGAYGESVSDALLD
                     VHLLEEGKEHVVGPDRLDFGYRTSLLKSRRDIVVLSASFQLHHGHRHELLRRIADDAA
                     LRKSKHPLEYASCGSYFKNPSRELPAARLIEDAGLKGLRVGRAHVSEKHANFLVAEPG
                     ASADDITTLAAEVVRRVKETSGYELTEEVRRLGFA"
     gene            33988..34494
                     /locus_tag="VFZ86_09495"
     CDS             33988..34494
                     /locus_tag="VFZ86_09495"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX5642555.1"
                     /translation="MRRGQPEARAELFRLYAPQVRRILYLQGFCEEVDDAVQEVFIKV
                     YRAREIPREEAFLAWFYRVILNTGRDHGRRRKTRYGLMDKLQQIAPEATVDPPSEPAD
                     PALRAALATLPDELRECVALRFFADLPLDEIARAQDIPVGTVKSRLHGAAKKLRAALD
                     EQGYEREC"
     gene            34526..>35156
                     /locus_tag="VFZ86_09500"
     CDS             34526..>35156
                     /locus_tag="VFZ86_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642556.1"
                     /translation="MTDERTEYGMPAAGGFDDDRLLAYALGLEDDRELLQAAAADADL
                     GERLAAMRADVDAIGAQVRAAVPEPDASYADLSGERWASLREYLEVPASAAAPRRGRR
                     WWRVVAPVAALAVLALVAGTVALDQGGQPDSIGSSAEVVRTATDTSDSFTGQSESTTS
                     GEGAAADPLTAPGTETTPGAAVTPPPEEAPQTAAQSLAEQLDRFAVVVLA"
BASE COUNT         5084 a        12798 c        12408 g         4866 t
ORIGIN      
        1 atcagcggcg agaagaagca gctgcacgtg atcgacgcgg aggactgcat caagtgcggc
       61 atctgccgcg acgtctgccg gttcgacgcc gtgctggtgc agtaggaggc cgtcgtggac
      121 gcagagctca tcatcgacgg ccagaccgtc accgcggcgg agggctcgag cctgctgagc
      181 gcggcgcgcg ccgccggccg ggacgtgccc ggcctctgcc atcacgaggc cgtggcgccc
      241 atcgccagct gccgcctctg cctggtcgag gtccagcgcc cgggccggga ccacgtgcac
      301 ctgacgacct cgtgcgacta ccccgtgtcg gccggcctcg tcgtggtgac ggactcggcg
      361 cgcatccgca cgcaccgcac catgaacctg gagctgctgc tgcggcgcgc cccggaggcc
      421 ccggtgctgc tcgcgctcgc cgcggacctc ggcgtgacca cgccgcgctt cgcgccgctg
      481 gcggacgcgt cgctcccgga ctgcatcctc tgcgagctct gcgtgcgcgt ctgctcggcc
      541 ttgggctaca acgcgctggc ggcgatcggg cgcggcgagg ccaagagtgt gggtccgccc
      601 ttcgggttgg ccgaggcgcc ggactgcgtc ggctgcggtt cctgcgtgga ggagtgcccg
      661 accgactgca tcccgatggt ggataccccg acgacgcgca cgatctgggg ccggaccttc
      721 gcgctcaagg cttgcgtgcg ctgcggaagg ccgatcacga ccagggagca cgcggccgcc
      781 atggatgcgg cctggcagtt gccggagaag gatgagggcc tctgcgccgt gtgcaagaaa
      841 cgcgtcgtga gcgagcgcct cgccgcaccg ggacgctgac acgtacgcag tgacggccgg
      901 cgcatcggcg ccggccgtca cgtcacttca tccatgcggc gggtgcgtcc ccgtcctggt
      961 gatcgtcgcc cgtctgaaaa gtctgttccc gccggcagcc tcactcacac cctcaccccg
     1021 gtgaggcggt cgccgcgaac gaccggttcc ggccggcgcc gcggacgagg cagccgcgca
     1081 ccccgacgag gactcgctca ggtccggccg gagtcggccc tctcagcgat cgtcggtcgg
     1141 gacgccgaac tgccgcagga tgcggcgcgc cacagccgtg agcgcccagc cgagagcggc
     1201 cacggccagg agcgcgacga cgacggcgac tgcctgcacg acgcctacag gtacatcagt
     1261 gcggcgagga ccgccgcgcc cgccggcgcg acggcggtcc agaggaagcc gaagccggtc
     1321 gccacgtcct cggcgcgagc ctcggccgcc acgtccggat ccggctcgag tgcgctctcg
     1381 cccgcaccga gcgtcccggg gtcgaacggc ccaacgggac gtgcggcgga gacgcgcgcc
     1441 acgccgggcg gacggtcggg atccgccccc gtcaccgacc cgcggaacag catcaggagc
     1501 aggacggcga gcgcgccgtt ggcaagcacg aaccatcccc agctccaccc cacgaccgcc
     1561 aggacgatcg cgacgacgcc gaccaggacg caccctgtgg ccagcggcag cagcgcgcgc
     1621 ttcatggctc tcccttcctc ggccccgtga gggcctcgac ctcgatgaag atgcggctga
     1681 cctcggggta cggcccgctg atgcgcccct cgacgcgatg cacggcatcg tggatctcct
     1741 cggcgctcag gccgggcgag aagacggctt cgatgttgag cagcacgtcg tcgggcccga
     1801 ggtgcatcgt cagcacggga ccgacgtcgg ccacggcgtc gtccgccagg atgaggcggc
     1861 ggatctcggc gaccatctcg ggatccgccg cctcccccac cagcaggctc cggctctcgt
     1921 aggcgagcca cagcgccgcg accaccagga tccctccgat caccaccgag gtgagaccgt
     1981 cgatcaccgg ctcctcgagc tggtggctca ggaagatgcc cacgaaggcg accgcgagcc
     2041 ccagcaacgc ggccgtgtcc tcgaacagca cggtgaagag gctgggatcc ttgccgtgat
     2101 ggatcgccgc cagggtcctc ctgcgaccct tgctcttgcg gaagatgcgc caggcgacgc
     2161 tgaaggagta gctctcgaag agcatggcga cggccagcac gatgtagttg atcgtcgggt
     2221 cctggagcgc cacgggatgc tggatgtgcg tgatgccctc gtagatggac atgccgccgc
     2281 cgatgccgaa gatgctcacg gcgacgatga ggctccagaa gtagacctcc atgccgtgcc
     2341 cgaacgggtg ctgctcgtcg gccggcctgg ccccccgctt gagcccgtag aagagcaggc
     2401 cgccgttgcc cgtgtcgacc agcgagtgga tgccttcgct gatcatggcc gagctgccgg
     2461 tcatggccgc ggcgatgaac ttgacgacgg cgatcgccag attgccgatg atcgccgcga
     2521 cgaccgcctt cttcgattcg gctgccattg agacggagtc tactgcacgc gggcaggcga
     2581 acccggtcct cacccgaatt acaggacgac ggtttggggt atgtatgtgg tcgtggccct
     2641 tgaagggagt accacgatgg ctggaagcag cgagtatgag tgcggcgagt gccgtcaccg
     2701 cttccgtgcg ccggagggag tgccgcgcga cgcgcggacg ctcatgtgtc ccgcgtgcgg
     2761 cagcatcgac ctcaacctca tgagcgtgga gcggccgcgc ccggtcgtga tggtcccgcg
     2821 cgcgggcacg ccctccccgc tgtggcggga gccgagggag accaaggcct cgtagcgcca
     2881 ctcggtggtc gtcgcccccg gccacggagc cgaccgggag cgccgtccgt cgcgtacggt
     2941 ctggtacgct aggatcatgc tgagggccgt cacgttcgac ttctggagca ccctgttcgt
     3001 cgactcgcgc gggcgtgagc gagaggcgct gcgcgcacgc gtccttgcgg atacgctgtg
     3061 cgccgccggg ctgcaggcct ccggctacgc gctggacgag tcgctccgcg caagctggga
     3121 ccacttcgac cgggtctggg cgcgggagca ccgcacaccg ccgtgcgccg agctcgtgga
     3181 cgccatcctc ggctcgctcg gcgtcacggt gccggacgat ctgctggtcc cgctcgtcga
     3241 ccgcttcgag cgtctcgtcc tcgaggtgcc gccggagccc atgcccggcg ccgtctacac
     3301 catgccgcag ctcgccgagc gctaccggct ggcgatcatc tgcgacacgg ggtactcccc
     3361 cggcgccgtg cttcgtcaac tgctggaccg ccacgacctg ctcggccact tcgagtacgc
     3421 ctacttctcc aacgagcacg gcatgagcaa gccggacccg cgcgtgttcg tgcacgtgct
     3481 cgaggagctg gacgtgcgtc cagcggaggc ggcccacgtc ggcgacatcc agcgcaccga
     3541 cgtcgccggg gcccaggcgg ccgggatgtc cgccgtgcac ttcgtcggcg ccaacaacgc
     3601 cgacgtacct cactccacgg gagatgccat cgtcagccac ttcgaagagc tgccgcaggc
     3661 gctcggcagt ctgatctgtg ccggctgctg agcgccctcg ccttctcgga cggcagcgcc
     3721 ggccgtacct gatcgccgca ctcgccggtc tggccgtgtt cgtcctcgtg acgatcctca
     3781 tgcgcaccgg cgcgctggac gggctggaca cccgtgtggt cacggacttc gccgggggcc
     3841 gcagtcagcc gctcaccggc atcgccgaga cgctggacgc gctcggcgcg tggtggctgc
     3901 tcctcctcct ggtcgccgtc ctggtggggg gactgtggtg gtccgggcgg atggtgcagg
     3961 cgctgtacct ggcggcgagc gtcgcggctg ccgtgatcgt caacccggtc ctcaagcagg
     4021 tgttcgcgcg cacgcggccg gaggacgacc agctcgtcac cgtgtccagc acggccttcc
     4081 cgagcggcca cacgaccgcg tcggcgacca tcgccaccgc gctcgccgtc atcgcctggc
     4141 ccacgcgctg gcgctggccg gtgatcgcgg cggcggctgt gttctcgctc gccatggccg
     4201 tctcgcgcgt gtacctgggc gtccactggc cgtccgacgt cgtgggaggc ctcgcgctcg
     4261 ggttcaccgt cgcgatgacc gtccggctgc tgatgccgtg gccgtcgccg gaggaggccg
     4321 aggccgcgcg gcaagcggcc ggggcggccg cgcccgcggc ctccgacctt cccgggatcg
     4381 acgtcgtctt cctcgactgg ggcaacacgc tcatggtcga cagtggcatg cgcgaaggcc
     4441 cgatgaagga ttgggacacg gtggaggcgg aggccggcgc aaaggaggcg ctgctccgcc
     4501 tgcgcggccg gtaccgtctg gtggtcgcca ccaacgcgga ggactcgccc gctccggacg
     4561 tgcgcttcgc gctcgcgcgg gtcggcctgg acgagtgcgt cgacgaggtc atcacctcgg
     4621 cggacgtcgg ggaccacaag cccaactacg ccttctaccg cgccgcgctc ctgcaggagg
     4681 gcgtccgcgg gcttcccctg gacccacgcc gggccgtcat ggtaggggac ggcaccacca
     4741 acgacatcgc cggcgcccag cgggccggca tgcgcaccat ctggtacaac cccaccaagc
     4801 gccggttccc agacggggcg ccgccgcccg acgcggtgat ccgcaagctc gccgatctgc
     4861 cggcggcggt ggacaagctc gccggcgtcg cgccggagaa acggtcccgc aagcggagcg
     4921 aagcggagac ggcggcggcc gcagccgcgg cagcggcggc cgccaaggac ttgcgcgccg
     4981 agaccgcagc agaggattcc gcggcgtcgg ccgcggccgc catccccgac gggaggtagc
     5041 aatggatctg tcacaccccg agcagtggct ccgccgggca ctccagctgg gcgtcgacgc
     5101 agccacgccc atcgatgcag acgccgtcgt cgccgccgag tgggtgcgca tgaagtgcct
     5161 ctacgggtgc gatgagccgg gcatccgcaa gacctgcccg cccaacctgc cccccgtcgc
     5221 ggtcacgcag cgactgctct cggaataccg ccgtggcgtg ctcctcgagg tcggtcccat
     5281 cgtcggccgc gaggccagcg accccgagtc ccggcgtctc aacgacgccg ccctcgccct
     5341 ggagcgcgac ctgttcctcg cgggccacca caaggcctgg atgatgggcg ccgggccgtg
     5401 cgacctgtgc gacgcctgcg gcacgggcag ggagtgcccg acgccggaga aggcgcggcc
     5461 ctccctggag ggttgcggtg tggacgtctt cgccaccgtg cgcaacgccg ggcgcaccat
     5521 cgacgtggcg tgggacgagg acgacgagta ccgcttcttc gccctcgtcc tcgtcgagta
     5581 ggtccgtcag gccgcctgcc gcgtctcggg tgaggcggcc agtcccaggt ggtgacggca
     5641 gagctcgagt ccgccgcccg tctcgagcca gctggagaac gtgtgcaact gcgggtggac
     5701 gcgccgcagc cactcgatgt cgtgcaccgg cgtcgaggcc tcctggtaca cgaacacgcc
     5761 ctcgacctgc acctcctcgt accgcaccgg ctcgccgagc gcttccgaga gccgtgcggc
     5821 gatgtcgagc ggcgtccgcg tgtcgccggc cacgtcgatg gtgcgaccga cgaactcctc
     5881 gccgtgggtc accgcgagcg cgaccagggc gcccacgtcg tccagcgcca ccagcgggag
     5941 gtggttgtcc gccccgaacg gcgtcgccag cgtcagttca cgtccgaacc ggctgagcca
     6001 gtaccacggc acctcctcca tgaaggtggc cggccgcagc accgtcgccg gcacgccctg
     6061 ccggacgagg taccgctccg tctcggcgct gacgtcacac gacagccggt ggtggtccgg
     6121 ccccgtggcg gcggtgaaga cgatgtgtcc gacgcctgcc cgcgacgccg cttcgatcat
     6181 gctccggccg tgacgcaggc gctcggccgg gccggtgtcc gggtcttcca ggatgaggac
     6241 gagcgcgccg gcgccccgca gggtgcggtc cagcgccgat ggatcgtcga accgggccgt
     6301 gcgcgttccg gggacgtccc agcgcggggc gtgccgacgc gcgtcgcgtt cgtcgaagag
     6361 ggcgcgcgcc tcgacgtcga atgcgccaag ccggtcgagg gtggcccgtc cctgatggct
     6421 ggccgcaccc accacgtaga ccggccccga tgtggtcttc atgagactca cccgcctcca
     6481 agctcacggg aactgcttcc gataccgctg ttgttcccgt aacggctcgc agcggggcgt
     6541 tggagactct ggggactccc acggacgacc gtccgcaccg cgaggcgcga tcagacgagc
     6601 agcgccgccg tctgctcgag ccgggcgacg gcctggtcgc ggccgagcac ccagaggctc
     6661 tcgaagagcc cgggggtgac gctctggccg ctcatgccga gccgcagcgc ggcgaacacg
     6721 gctttgggct tgagctcgag cgtcgcgggg aggtccctga ccagcgactc gatggcgggc
     6781 acatcccacg gctcgaggtc gcgcagaccg gcggcggcgg ccgcgagcgt ctccgccgct
     6841 ccgcccgtgc cccggagccg ctcgagcgcc tccggcgcga acgcgagcgg ccggaagaac
     6901 catccggcca tggggacgaa gtcggcgagc acctcgagct tctcgtgcac caggggcgcg
     6961 acctcgcggg tgagcgcctc ctgctcctcg tcgggcaccg tcggcgggag gtgctcggtc
     7021 tcggcgccct cgactgcggc ggcgagggcc tgcggcccat gcgcagccag ctcgccgacg
     7081 ccggccgggt cgccgtagaa gccgacgcgc gtgaggtact cgacgaggcg gccgccgagc
     7141 cactcgtccg tcatcgcgcg caggtagcgc ccgttcatcc agcgcagctt ctccgggtcg
     7201 aagatgctcg ggctggtgcc cagcctggtc acgtcgaagt cccgcaccag atcgtccagc
     7261 gaccaggtga tcatcgtctc gtcgaactcg gcgctgagga agcagaggta gttgcgcacc
     7321 acctcgacga ggaacccctc cgcggtgagc tgctcgagct tcgtcgcgcc gtggcgcttg
     7381 ctgagcttct tcttgtccgt cccgaagaga agcggcatgt gcaggaactc cggcacgccg
     7441 gcgcccaggg cctcgtagat cacgacctgc ttgggcgtgt tgctgatgtg gtcgtcgccg
     7501 cggatcacgt gcgtgatctc catggtcacg tcgtccacga ccaccgccag gttgtaggtg
     7561 acggtgccgt ccgagcgctg cacgatgaag tcgcccatgg tctcgttctc gaaggcgacc
     7621 tcgccacgca ggatgtcgtg gaagacggtg cggccggcgg gtacgcgcac gcgcagcgtg
     7681 tgcggctctc cggccgcccg ccggcgctcg acgtcctcgg gcgccagcgc ccggcacctg
     7741 cccgagtaca cccagccacg gccgtccgcc tgggcggccg tgcgctcggc ctcgagctcc
     7801 cccggcgtgc agaagcaggg gtacaggtga ccgctctcga gcagcgtctc gacgtactcg
     7861 tggtagatgc cgtcgcgctc tgtctggcgg tacgggccgt gcggccccgg cacccccggc
     7921 ccctcgtccc agtgcaggcc catctccttc atggaggcga tgatctgcac gatcgccgcg
     7981 tccgtactcc gactgaggtc ggtgtcctca atgcggagga tgaacgtccc ctcatgttga
     8041 cgggcgaaga tgtagttgta gagtcctgtg cgggcactgc cgagatgcag gtagcccgtg
     8101 ggtgaaggcg cgaaacggac gcggatgtgc gagtgctcgg acattgaccg cccatgatac
     8161 cgcatcaccc ggagcggtgg ccgggcaacg gctttccata tcgaggaaca agactacaat
     8221 ggccagatgg acacagaagt gaacagagtc cgagtgatcg agtcgcgacc ccgcgaccac
     8281 gtacgaaacc tcagcgtccg agtcggcgac atggtcggcg tcgggcaccg caaccagcag
     8341 ttccccgagc tgcgctggtg cacgcccgag cgcggctact ccggctggat ggccgagtct
     8401 tacttcgagt acaccagccc caccgaggcg atcgtccgga aggagtacga cgcttcccag
     8461 ctcaccgtgc tcgaaggcga ggagctcgac gtcctcgacg tcgtcggcga gtggtggctc
     8521 tgccgcaacg accgcggcat caagggctgg gtcccccaac gcatcctcga ggacgccacc
     8581 cccggcgact cctgactgga gtgaccatga tcaccgattc gaaaagccgg caggtccggg
     8641 tcgtcacctc atgcacgcgt gagcaggtgc aggagatccg catgcgcaaa ggcgactccc
     8701 tggccgtcgg ccaccgcaac cagcagcatc ccgccttcat ctggagcgcg accgaggacg
     8761 ggcaccacgg ctgggtgccc gaggactacg tcgaggtcgt cggcggccgc gacgccgtgg
     8821 cacggcgcga ctacgactcg acgcacctca ccgtgagcag cggcgagcgt ctcgaggcga
     8881 tcgaacaggt gggggactac ctcctttgcc gcaacgcggc gggcgtcgag ggctgggttc
     8941 ccgagtcgtg cgtcgaggag accgtggagg aagcatgacc gaggcgcaac tcccgcggcg
     9001 cgtccgcgtc atcgagccgc acgtgaccaa cgacccgaac ccgatccgct tccgcgccgg
     9061 cgacacgctc ggcgtcgggc accacgacca ggtctggaag cgctacgtgt ggggcaccga
     9121 ccaggcgggc aggtccggtt gggtgccgga cacctatctc gagttcggcg ccgacggcca
     9181 cacggcgatc gccctgcggg actacgactc gaccgagctc tcggtcgggc gcaagcagat
     9241 cctcgaggtg ctcgacgagg ccggtggctg gtacctgtgc cgcgcggagt cgggctcgtc
     9301 cggctgggtg ccgggcacat ccgtcgagcc cgtggagccg gccgagccta cctgacgcgg
     9361 atggtcacgc gcccggacct gaccgggccg aggaactggc cggcgccggc gaagcgcacc
     9421 tcgtaggcgc gcgtgcgcgc cggcctcagt gtcccggtcc acacgccgtt ctcgccggtc
     9481 gtcaggcgcg ccatcacctt gccgtcgacc aggcgcacca gccgcagcgg cgtcaggcgc
     9541 gccaccggca ggccggcact cgtggtgcgc acgtagacgc gcgccgctga tccggccgcg
     9601 acggtgacgg cggtcggggt gaacgccgcg ttcgtcgcca gctcgagggt gaccggcgtg
     9661 gggccggccc acaggtccct ggtccggccg gccgtctcac cggtcgtcgc ctccagctcg
     9721 aggtcgtggt ggcccggcgt gagcggctcc gtcgtcagcg tccatccctc cgacggctcg
     9781 tcgaacgcgc cgtcggtggc ggtgagcggc tgccaggcgc cgtcgtccac ccggtaccgc
     9841 gcctcgtgcg gcaccttgat gctcaagtcc ctgcggaagt agacgccgcc gctgatgcgg
     9901 ccccggggac ggggctgctc cgtcaccgtg ccgcgcagcg tcacgacgcc acccgcgtcg
     9961 gtcgactcga gccgtgtgga gatcccaggc cgcgtgtcca ccacgtccgg ccgtgtgtcg
    10021 gcgtcgctgt cgcgcaagcc cgtctggccg gcggtccagc ggcagagatc accgtcgcgg
    10081 aaggcggcga gcgtcgcgtt ggatccgccc atgatgcagg gcaggtcggt cgtccctccg
    10141 cgcaccgcgt tgccgttgcg cacgccgagg tagccggacc tgaggtcgcc ggtgctcggg
    10201 tagccgggaa cgggcggggc gtactcgtcg agcgcgccga acacgtggcc catctcgtgc
    10261 gccagcaccg cgtcgaagct ggcgaagtgg taaccgtcgt tgtcgtaggc gagcgtgcag
    10321 tatgggccga agaggtcggc ggtgtaggcc accacgcggt tgcggaacat gccgtcctca
    10381 tcctgcaggc tgtcgaccac gtagacgacg aaggcccaat ccgcgccgtt ccgcctgcgc
    10441 agccggtcgg cgaagagcgt ctccgggtag ggcgtgtcat cggtcgcgtc gtgggcgaac
    10501 cccagcctgc ccatggcctg ccagcgccag gcggcatccg acagcaccgg ccggcccttc
    10561 cagctcaccg ccacgtccct cctgatgggc tcgtagcccg tcggcacggt ctgcggcgcg
    10621 ccgtaagagc ccgccgccgg caggaacagc tcgagggagc cgtccggcga cctggcgttc
    10681 caccagtcga gcgccccctg gatcatggcc aggacgagct ggcgacgatc ccggccgggg
    10741 tagtccgggt cctggcgcgt ccaatcctca ctgctcgtca tgatgctgcc gtcgctctcg
    10801 aggaagacga cgccgacggc cacccggccg gcggcgaagg cggccgtctc gcgggcgccg
    10861 ggcagggcgc cgaggccgat ggggagggcg ggcgcggcca cgccgtcccc gcgccccatg
    10921 agatcgtcga gcaccggtgg gaccggcttc accggcccgg gcgctgtcgg gtcgacactg
    10981 ccggccgcgc tcgcggccgc cggcagcccc aagcccgctg caagcaggac gctcgcgaga
    11041 agggcgcacg ccagcagcgc gaccagcccg ggcgcgacgc gacgtgactg acggccccgt
    11101 gtcatggcga cgattgtcgc acggggccgt cagccggtcg actcaggtcg tcgccaggtc
    11161 gatcgtgaac gtgctcccct cgcccggctc gctctcggcc tcgacgctcc ctcccatcgc
    11221 ctcggtgagg tccttgacga tggccaggcc gaggccggtg cccacggtcg gcccggtgct
    11281 gtacttgcgg tgcaggtaga accgctcaaa ggcgtgatcg agatccttct cggtgagccc
    11341 gggacccgtg tcgcgaacgc tgagccgacc gggcctggca acgacctcca cacgaccgcc
    11401 gctgctcgtg gcccgaagcg cgttctccac gaggttcgag gcgacctgca gcaggcgccc
    11461 ggcgtcgccg cgggccggcg ccggctccgc cgcgtcggcg accagcgcga cgcccagaga
    11521 cccggcgaga ccctcgaacc tggcgaccgc ctcgcgagcg gtgtccgcca ggtccacggg
    11581 ctcgtccacg acggtgaact cggaacggtc gatgcgcgcc aggtcgagca ggtcggtgat
    11641 caggcgccgc agacggccgg actccgcgct caggacctcg gccgcctccc ccggcgcgac
    11701 ggccccgtcc tggagcagct cggcgtagcc gtcgatcgcc gtcagcggcg tcttgagctc
    11761 gtggccgacc gacatcagaa agtcgcgctc ggtgtcctgc gccttcttca gcttgcgggc
    11821 catgcggttg aaggactgcg ccatcaccga gagttcgagc ggtgccttcg tcgggatcgc
    11881 cacagggatc ccgccgtccg ccagtaccac gctggcgtgc gcgacctgct ggacaggctt
    11941 gacgatgcga cggttcagat accacgccgc gaccgcggcg aacggcgaga gcagggcgag
    12001 gccgatgagg agactgagca ggtactgcac cacgtgcgac caccatggcg ccgggtcgta
    12061 gacgtagagg actgcgcgct cgtccgacgt gcgccaggcg acgtagcgcc aatcgcggtc
    12121 ctcgggcccg accaggcctt gcccgacgtc ccagcctctc tcctcgagcc gggcggtcgt
    12181 ggcgggagtc agatccgtga tcatcgtccc gtccaggaac accgtgccgc cgccggagcc
    12241 ttgtgcgtca gtggcatcga gcagcgcctg acggatggct gcgtgggcct catcgtcgat
    12301 ccacctcgcc tcaccgcgcg agatgaagcg gtcgagcttc gccaacccct catcggctgg
    12361 ccactgatcc tcacgtggct gctgccggtc cacgatcacg atcacgagca ggaagagcac
    12421 gagcagcgtg acgaggacgg tgcggaagag cgtggtgcgc agcggcgcat gctcgggagc
    12481 atcctcacgc aacccctgcc ggaagccgcg ccggaagccg cggatcccga cgaacgacag
    12541 tacgacgatc acgatcgcgg caatggtcaa gatgatgaga gggatgatga tcatcgcgac
    12601 accaccttgt agccggcgcc gcggaccgtc tcgatgaacc cgggcaggtc cagcttggcg
    12661 cgcacctgcg ccacgtgctg gtccacggtg cgcgtgccgc cggggaactc gaggccccag
    12721 acgcgctcca gcaagagctc gcgcgagaag acgagaccgg cgttctccat gaggaaggcg
    12781 agcaggttga actcggtcga gctcagctgg acgggccggt cgccgatgtg cacctcgtgg
    12841 cgcgaccggc tcaagctgac gtcgccgacg gtcgtggcgt cggcgcgcgg ggaggcggtg
    12901 ctgcgccgca gcaccgcctt gacgcgcgcc gccagctcgc gcgggctgaa cggcttgggc
    12961 acgtagtcgt cggcgccgag ctcgaggccc acgacgcggt cggcttcctc gtcgcgcgcc
    13021 gtcagcatga tgatcggcag gctcgagccc gcccggatgc ggcggcacac ctcgagcccg
    13081 tccatgccgg gcagcccgag gtccagcacc acgagcccga cggggtggcg cggcagctcc
    13141 gccagcgcct cctcccccgt gcgcacggcg acgacgcgat gcccgtcgcg ctccagcgcg
    13201 atgcggacca cggtcgcgat gttgtgttcg tcctcgacca cgagcacggt ggtcatgtcg
    13261 cccctcctcc tgcggtggac agatgctacc agcaggccca tcctcgcttc gccgcgtcgc
    13321 caccctgtca cgggcatgtc agagcggtgt aagagcgtcg ggcataccgt gagggcgcac
    13381 cgcgcaacgt gcgcgcgcga ggggcatctg gtaaccttcc ggcttcgtga acgtggctca
    13441 tcacacactc gatccctcga ccctggacgc ctgacatgcc gcagctgcgc atcgtcgtcc
    13501 tcgtcaagca ggtcccggac acggccaacg tgaccggcga ggccatgaaa gaggacggca
    13561 ccatcaaccg ggcggcgctg ccggccgtgt tcaaccccga ggacctgaac gcgctggaag
    13621 aggcgctcaa gctcaaggac cgtcacgacg cgcgcgtcac ggtgctgacg atgggaccgc
    13681 cggccgccgt gcaggtgctg cgcgagtcgc tgtaccgcgg cgccgacgag gtcatcctgc
    13741 tcacggacaa ggcgttcgcc ggcgccgaca cgctcgcgac cagctacgcc ctgtcgctcg
    13801 ccgtgcagaa gctcggcggc gccgacctcg tgctctgcgg ccgccaggcg atcgacggcg
    13861 acacggccca ggtcggcccg cagacggccg agaagctcgg ctacccgcag atcacctgcg
    13921 tgagccgcgt cgacgagctg cgggcgccgg acggcgaagc cggctccggc gctggaggcg
    13981 aagccggcag catcaccgcc acgcgcagca tcgagggcgg cttcgagacg ctcacgagct
    14041 cgctcccgtg cctgctcacc gtcaccggcg aggccaacga cccgcgcccg ccgagcgtca
    14101 agctcgtgat gacctacaag ggcatcgccg ccgaggccat gcccgagtac gacgacagct
    14161 acctggatcc cgagcgtgac gaggccgagg tgtgcatgat cggaaaggac ggcaagcccg
    14221 tctgccgcgc ccgcgtcatc cagtgggacc acgcggcgct ggccgccgac cgcgaggcgc
    14281 tgggcctgcg cggctcgccg acgcgcgtca aagagatcga gagcgtcgtc ctcgcgcaga
    14341 gcgaagcgcg catggtcgcg cccgacgagg ccggcatcac cgagctcgtg cacgagctga
    14401 tcgaggagca catccttggt tgatacagcg aacaccaacg acgtctgggt cgtcgtcgag
    14461 cagcacgacg gtgaggcggc cgaggtcagc ctcgaactgc tcggccgcgc ccgcgagctg
    14521 gccgaccagc tcggcgtgca ggtcggcgcc gtgctcatcg gggcgggcga ggccgccaag
    14581 gcccttgcgc ccgccctcgt cgcccgcggg gccgacaccg tctacttcgt cgcccacacc
    14641 gacctcgcgc actatctggc acagccgtac acccaggtcg tcaccggcct catccgcgag
    14701 cacgagccgc agatcgtcct gtacggcgcc accaccaccg gccgcgacct ggcgccgcgc
    14761 gtggccagcg ccctgcgcac cggcctcacc gccgactgca ccgacctgcg catcggcgac
    14821 cacgcgctca agggcgagga gttcaaggac ctcctgtacc agatccggcc cgcgttcggc
    14881 ggcaacatca tcgccaccat catcagcccg ctgcaccggc cgcagatggc caccgtgcgc
    14941 gagggcgtca tggtcatgcc cgaggaggac cgccggcggg acgggcgcat catcgccgtg
    15001 cccgtcaccc gggccaggga gcgcatcgcc gtccgcgccg gcgtcgtgct gagcgacgag
    15061 gacttcgtcg tgacgctggt gaagcgcgtc caggaggaga agcgcgtgga cctcaagggc
    15121 gcgcgcatcg tcgtctccgg cggggtcggc gtgggctcgc gcgagggctt cgcgctggtc
    15181 gaggagctgg cccgcacggt cggcggcgtc gtcggcgcca gccgcgccgc cgtggatgcg
    15241 ggctggatcg accacgatca ccaggtcggt cagaccggca ccaccgtccg acccaagctc
    15301 tacatcgcct gcggcatcag cggctccgtg cagcaccgcg ccggcatgga ccagagcgcg
    15361 cgcatcctgg cgatcaacaa cgacccgctg gcgccgatct tcagcgtcgc ccactacggc
    15421 gtggtcggcg acctgcacaa ggtcatcccg ctgctgatcc aggcgtacaa gacgaagggc
    15481 gcgggcggcg gagccgctcc cgcgcccggc caagccgcgc ccgcccagcc cgccgcggca
    15541 gacggaggtg cgtcgtgagc aactacttcg gcgacaacgc cgacctccag cagacgctgc
    15601 gccgcctcga cctcgcgcgc gtcgtgcgcc tgcgcgagga cgactacgcg caggcacgtg
    15661 acttcgccta cgcgcctgcg gacttcgagg acgccctcga ctcgtacgcg cgcacgctcg
    15721 agatcgtcgg cgacatcgcc ggagagttcg tcgagccgcg cgcggaggac gtcgaccgcg
    15781 ccggcgccga gctgatcgac ggcgaggtcg tgtacgcccc cggcatcacc gagagcctcg
    15841 aacgcctcac ccaggccgag ctcatgggca tcaccctgcc gcgcaagtac ggcggcctca
    15901 acttcccggt ctccgtcagc gtcatgatcg tcgagatggt cagccgcgcc gacccggcgc
    15961 tcatgaacat cttcggcctg caggacatca gcgagaccgt caacaagttc gccgacgacg
    16021 cgatgaaggc cgcctatctg ccgcggttcg ccagcggcga ggtcaccggc agcatggcgc
    16081 tcaccgagcc cgaggccggc agcgacctcc agaacgtcca gctcaaggcc accgagcagc
    16141 cggacggcac ctggcgcctc aacggcgtga aacggttcat caccaacggc aacgggcgca
    16201 tcagcctcgt cctcgcgcgc tcagaagagg gcaccaccga cgcccgcggc ctgagcatgt
    16261 tcctctacga gcgcgacgag cacatgcgca tccgccgcct cgaggacaag ctcggcatcc
    16321 acggctcgcc cacgtgcgaa ctgcagttcg acgaagcgcc ggcgctgctc gtcggggagc
    16381 gccggcgcgg cctcacgacc tacgtcatga gcctcatgaa cggcgcccgc ctcgccatcg
    16441 cggcgcaggc gcagggcatc gccgaagccg ccttccgcgc ggcggccaag tacgccgccg
    16501 agcgcatcca gttcggcgcg ccgatcggcg agctcaccgc ggtcaagggc ctgctcgccg
    16561 acatgaaggt gagcatcgag gcggcgcgcg cgctgctcta cgagaccgcg ctcatcgtcg
    16621 acaccaagga agtcctggag caccgcatcg cgcagctgca ggcggcggag aaggccggcg
    16681 aggctccgcc ggccggcccg gacaccgacg tcaagagcct ccgcgccgag ctcaagcagt
    16741 acacgaggct ggccgcgctg tacacgcccc tggcgaaggc ctgctgcacc gagatggcca
    16801 accaggtcgc gtacgactcg ctccaggtgc acggcggctc ggggtacatg cgcgacttcg
    16861 ccgtggaacg gcacgcccgc gacgcgcgga tcaccaacat ctacgagggt acgacgcagc
    16921 tgcaggtggt cgcggccatc ggcggcatcc tcggcggcac gctggccggc cggctggacg
    16981 agtacgacgc cgaggacttc tcggccacgc ccgatctgct cgcgcgcgtg cgcggcgcgc
    17041 gggcgcgact cgccgaggcg atcgctcggg tgcgcgacac ggatgacgtg cgcttccgcg
    17101 acttccatgc ccgccgcctc gccgagatgg cgatcgacgt ggactgcgcc tatctgctgc
    17161 tccgaccggc gcagaccgac gcgcgccgcc tgctcgcggc ggagtacttc gtcgccgcgg
    17221 ccgcggcgcg cgtggagggc gccgccgcgc aggtgctcac cggcgacccc tccacgctgg
    17281 acgcgctcac caccctggcg gccggctgag cggacgagcc cggcagggcg ccggaccagc
    17341 gagccgccgg tcaggccggc tcgtcgtccg gcgcctcgtc ctccgcgtcc tcctcgggct
    17401 ccggcggcgg gggctcgaca cggtcgccgc cggccgcccg cgccgccgcg agccggtcct
    17461 cggcaacgcc ggccaggtcg tccgcatcct cggcgtcctc ggggaacccc gccacgccga
    17521 agctcatggt gaaactgccc aacgcgccca gctcgtcgtt ggagagcctt gtggcggcca
    17581 cgcgctgacg cacccgctcc gcgaccagcc ccgcggcatc gggccccggc ggcgtgttgg
    17641 gcagcaggag cgcgaaccgg ccgccgccga ggcggcacgc cacgtcgacc ttgtcgcgca
    17701 ggctggtcat cagcagtcgc gccagcgcgg cgagcgcggc gtccctgaag gaggccccgc
    17761 gcctgcgcgc cagctcctcc tcgccgtcga gctcggcgac cacgagtgag agcggctggc
    17821 ggtaccgccg cgcacgagcg acctcggcgt acagccgttc gtagaagtac cagcggctgt
    17881 acagcccggt gaggacgtcg gtggcggacc ggctctgcac ggcgcggaac gccttggccc
    17941 gctcgagcgc agccgcggct tggccggcga gcagtgacgc gagctcgatc tccgcctcgc
    18001 ggaacgggcg gcgcagcgcc gccgtgaact cgaggtagcc gccggcgcgc tcgccggccg
    18061 tcagcggcag caggagccgg gcgcgaccgt cggcggtccg cgcctgctcc ggccgcccga
    18121 gatccgcggc ctgacgcgcc agggcgtcgg cgcgccaacc tccggccgga cgctccttcc
    18181 cggcctcgcc ggcgggagcg aacgtgccgt cgtcctggcg cagcatcacc tggacatcgc
    18241 agaccacccc gctcacgagc cccgcagcct cctcgcgcac cgcggcgacg atcgccgccg
    18301 gcgactgcgt cgcggagatg ccgcgcccgg ctgcgagcat ggccgtgagc cgctccgtcc
    18361 gcgcgcgctc ggcctcatag agccccgacg cgcgcagcac cgcagccgcg aggcgggaga
    18421 gctccccgag acggccccgt tcctcctcgg agagacggtg cccggagcgc gtctcggcga
    18481 ggctcaggac gccgagtacc tcggcccctg cgaggagcgg gacgtcgatg cggtgggtga
    18541 agccggcctg ggcgagggcg gcggcgtcgg caggcggcag cccgtcgtcg acgtggcgtt
    18601 ccacgagctg ccccgtcagc accagcctgc gcaggtcgtg gctctggtcc aggccgatca
    18661 ccgcgccgac gcagccgcgc gtcttcgccg tcttggggtc gcgacaccag tacgcgcggc
    18721 actccagcgt gtccgcgtcg tcgctgaagg cccacaggtc cacggaccac aggtccagcg
    18781 cctcgccgac gttcgcgacg acggcctcga gcaggtcctc gacgccgcgg ccggccacgg
    18841 cccccggccg cgcatggtca tccatgcgtc tccgcccggc catcagcgcc acgtgcgacc
    18901 acctggtcgc ggccggagcg cttgccctcg tagaggaccg cgtcggcggc cgcgatgagc
    18961 gacgccgcat cgtcgccggc gcgcagatcg gccaccccca ccgtcgccgt cgtgtgcgcg
    19021 tagcggcggc cgccgtgccc cgggaaggcg tgcccggcga tggcggctcg cacgcgttcg
    19081 gcgacgatca gcgcgccggc gccggccggt ggcggcgcct ccgggtcgtc gccgccggcc
    19141 gccgacgcgc cctgagcggg cagatccgcg gcccgcgtgt gcgggaggat gacggcgaac
    19201 tcctcgccgc cgtaccgcgc ggcgatatcc acgcctcgcc gcgtgacggc gaagaggatc
    19261 tgccccacct cgcgcagcgc ctcgtcgccg atctggtgcc cgaactcgtc gttgaactcc
    19321 ttgaagtcgt cgaggtcgag catcaacaac gacagcgggg tgccgtagcg ctgcgcgcgg
    19381 cggcactcct ggcgcaggcg ctcctggaag tgccggtggt tgtagaggcc cgtgaggccg
    19441 tcccggatcg cctcctgctc ggcgcgccgg tacaggcgct cgttctcgag tgccgccgct
    19501 gcctggttgg cgagcacctg cagcgcttca cgctcgcccc gtacgaaacg gcggtggccg
    19561 tcggcctcga ccaccagcac cccctccacg cggtccttga tcgcaagggg gacggccagg
    19621 cccgaccgct cggcgccggc gcggtcgacc gtgcagccgg cggcgagcgc ctcctgcgcg
    19681 agctcggcgg cctcggcgcc gagagtccgc ggtgtcacgg ccggcgggca gccggcctcg
    19741 ggcagccaca cggcggccgt cgcgccgccg gcgagcagct tcgcggcgct ctcctgcagc
    19801 gcggtcatca cctcgcgccg gtcgaaggta gcggtcaggc ggcggctgac ctcgagaagc
    19861 tcgagcagcc gctcgctctc ctcctcctgc gcgcggaaca ggcgtgcgtt gtggatcgcg
    19921 gccccggcgg actccgcgag tgcaacgagc agctcgagct cgggcgccgt gaaccgccgc
    19981 tcggcgtcgg tcatcgtgag ccgcaggatg ccctgcgggg ctccagcggc gcgcagcggc
    20041 agactcaggc acgtcttctg cctccaggac tccatggacc ggcgccggtc gccgggcaag
    20101 gcggggtccg agatctgctc cagcaccgcc tcgggcgccc ccaggatcac gcgctcgccg
    20161 gggagatcgg ccagcgcgta caccgtcccc agggcatcgt cgtgcactcc ctccgggggg
    20221 cgtcccgggg agtgctcggc ccggtacacg atcgcgtcgt ggtgcgggcg gtactcgtag
    20281 accgtggcgt aggtggcgcc ggcggcctcc gcggcgagcc gcgcgacgcc gtccagcacc
    20341 tcatcgaggt gcacggacgc ggtgagcgtc ctgctcgcgt ccaggagggt gccgagccgg
    20401 cgctcctgct cctgctgctg cctgaacagg cgcgcgttgc cgatcgccgt cgccgccggc
    20461 ccggccaaca ggcgcagcag ctgtctctct tcgtcggtga agcgccggtc gcggcgcgac
    20521 tcgaccaccc cgaggacgcc caggggctcg ccgcggaaaa cgagcggcat ctccatgacg
    20581 gccttctcgt cgtaccggat catcacttcg cgctcgagcg ggtcgaggtc gtcgtcgtcc
    20641 acgtactcct cgagcaggtc gcccctgcgg atgaccgcat cgcgctccgg acggtcaccc
    20701 aggctcacca cggtcccgat gtagtccacg tctaccctgc ggagctccct cgaccacacg
    20761 gcgacgtacg tcatggtccg cgcctcgggg tcgtactcgt tgatgtcgca ccactggaca
    20821 tcgagcgcct cgccgacctg gcgggcgatc gtctggatca cctcgtcgag gtcgagggag
    20881 gacgcgatcg tggtcgccac ctcgaggacg gtgcggtacg gatcgcgagc ggcgcgctcg
    20941 cgggcgtcgc ggatctcagt caccgaacac ctccacccgg ttcttgccga ggcgcttggc
    21001 gaggtagagc gccttgtcgg cgttgcgcac cagctccccg gggccgccgg cgacgcccgg
    21061 gtaacacgcc acgccgatgc tcaccgtgat ccgggagtct ccgtccacgc cgggcagctc
    21121 cgacgcggcc accgagacgc ggatgcgctc gctcacgccc agggcgccct cttcgtgcgg
    21181 cgggggggcc ccgggcagcg aggagagctg atgcgcgagg cgcgtgccca ccacctgcgc
    21241 gccctcccgc gcggtgttgg gcaggacgat gacgaactcc tcaccgccgt agcgcgcggc
    21301 gatgtcgatg cccacgcgga tgtgcgtgcc caggatcctc ccgacctcga cgagcacgag
    21361 atcgccggtg gggtggccga agcggtcgtt gaaggacttg aagtcgtcga tgtcgatcat
    21421 cagcagcgac agcggcaggc cgtagcgctg tgcgcgagcg atctcctggt tgagccgctc
    21481 gtagacgtag cggtggttgt agaggccggt cagaccatcg gtgatcgcct ggcgctcgag
    21541 catgcggtac aggccggcgt tggcgagcgc ggcggccgcc tgactggcga ggatctgcac
    21601 gagctcgagc tcgtcgtcgc tgaaggcgga tgacgaggcg gcgacgatgt cgagcagccc
    21661 ctcgacgacg tcgcccgcga cgaacgggac gacgacgcgt gaccccccat cctcggcgct
    21721 cagcgccaca gcgcgctcgt cgacggcgcg gcggtggagc tcgtccagct cgaccgccgc
    21781 ctgctcgccc gcagccgcgg ccaccggacc aggctcgaag gggacgaacg cctcgccccg
    21841 ccggagcagc atcgccacgg cgtctccgcc gacgccgaac agatcggcga cgtcgcgccg
    21901 cacctgtccg agcacggcgt cggcgtccag ggaagagacc aggacgcggc tcgtctcgag
    21961 cagggtcagg agacgtctgt tctgctgctc gccgcgacgg tacagccggg cgtggcggat
    22021 ggaggtcgca gcctgttcgc ccagagcgcg cgcgagctcc acttccgacg cgccgaaggt
    22081 ccgctccctc tcggtctcga tgagcaccag gatccccagc cgctcgccct cgacgatgag
    22141 cgggacgttg aggcaggcct tctcgcccca ctgctccatg gacgcacgca cgtccccggc
    22201 gagggacgcg tccgagagcg tctcctgcac cacctcgccg cggcgaagga tctcccggtc
    22261 cgtcgggtag tcgtccagcg ggtagacgcc gcgttcgtcg agagcaggcg gcgcgccgtg
    22321 gacgccccag aaggcgcggc agacgatcgc gtcgcgcccg gcgtcgtact cgtaggcgat
    22381 gcactcctcg gtatcgagcg ccgccgccgc ggtccggcag atgctggtca gtgaatcctc
    22441 gagcgtgacg gccgacgtga tggcgcgccc cgtgtcgagg agagagctga ggtggcggtt
    22501 ctgctcctgc tgcagacgga agagccgcgc gttgcacacc gcgatcgccg ccggccccgc
    22561 gaggaggacg acgagctcgc agtcctcatc ggtgaagcgg cggccctccc gcttctcgat
    22621 cagcgtgagg cagccgatca cgccccggtc gcgaaagtgc agcgggacgg cgagtgtggc
    22681 cgtctcgccc cacgccagca tcgccgtgcg gtccgccggg tcgagatccg ggtcgtcgac
    22741 ggccgacagg acgcaggcgc cgccctcgat gatcggcacg tacccgggcc gctcgtccag
    22801 ggatatccgc gtcccgaccc aggcgaggtc ctcatccgtg agctcgcgcg cccagagcgc
    22861 ggccgcggtg agcgtcctcg tctcggggtc gtactcgtag aggtcgcact cccacgcgtc
    22921 gagcgcctgg gcgacgcgcc ggacgatgac cgtgggcgcg tcgtcgccgg cgagcgccgc
    22981 ggtgatctcg gcgccggcct ccatcgccag ccggaagggc tcggtctcgg cggcgctgct
    23041 caccgcaccg cgcgaggctc tgccggctcg agctcgctca gcctcgggca ccggtccgag
    23101 gccggcgccg cggccacgat gtcggcgaaa cacgggaaca cgctgacccc cccagtcgga
    23161 cgccgccgcc tcgcgtcgcc acagcttacc agacggtccg cggaccggga gacggcggtc
    23221 gctcaaggta tcggcgcgcc tgccgatact atgagcggta cccccttcag aagggccgga
    23281 ccggcgcccg tcgccggtcc ggccctttcc tttcccggcc cttcgtgccc gcgcccggag
    23341 cgcccggccc ggagcgccgg cgtggcgccg cggatgcggc ccgctctctg gccccggacg
    23401 gcggccatgt gctaccgttg ccgcaatcct tggggaggcc gccgtgatct actgcgtggt
    23461 acccccggag ctcggtgaag acgcgtacaa gcggctcgtc gagcactacc gggacaaccc
    23521 gaacgtcaag gtcatcatgg accgccgcca cggcgagcgc cgcacggacc gctccggcgg
    23581 cggcgagcgc cagacgcgcg accggcggcg gcggcgcgct ccgggctcct acgacatctg
    23641 acggccccgg ggcagaccca tgaacgaacc actgccgcgc ggcagcgaga cgagcctcgt
    23701 gctcacccac ctcatgcagc ccgccgacgc caactacctc ggcaacatcc acggcggcat
    23761 catcatgaag ctcatcgacg aggccgccgg cgcctgcgcc ttccgcttct gccgcaccaa
    23821 catcgtcacg gcggcgatcg accgcatcga cttccaccac ccggtgcaca tcggcaacct
    23881 cgtgcggctc ctcgccaacg tcaacttcac gggcaggacc tcggtcgagg tcggggtccg
    23941 tgtggaggcg gaggatctcc gcaccggcat ggtcacgcac accaactccg cctatctcgt
    24001 gttcgtggct ttgagcggca gcggcaggcc gatgcccgtc ccgcggctca cgccgcagac
    24061 cgacgagcag cggctctggt acgagaaggc ggcggagcgc cgccgcaagc ggctcgccga
    24121 gcgcgagccg gccgacgact gatccgccgc gagacgcggc ttctgcggcg ggccggcgac
    24181 gcaggcgcgc ccaggccgac ggccggtcgc cgcacgccgg tcaggcggcc ggcgcctcac
    24241 cctccaggcg cagcaactcg cgcagcgcca cgtcggccac gtccagctgc tcggtcgtgg
    24301 gctcgctggt cgtgaagagg cgctgcatct cgatgccggg cctgccgagc gtgcgcgcca
    24361 gcggatggtc ctggtggcgc gacatccagg agaacagctc catcgcggtg ccgatgctga
    24421 cgaggccggc gatgaaggtg gccaagggtt gccgctccct gccggcggcg cgcagcacga
    24481 ggttggtggc gaggctcgtg accaccatcg gcgccacgag attggagccg cagcgggcgt
    24541 gctccttgtc cgcgccggcc gggtcgccgc cgctctcgta ggcggcgatg ctcttgtgct
    24601 cggcgccgtg atagcgcgag agccgggagt ccctgagcgc gacgagcacg ggggcgaggc
    24661 tcacgccggc cacggccagc tcccggagca ccggcgagcc aagcctcgtc gcacgcagtg
    24721 cgaccgtgac cgcggcgctc gccgccgtcg ccgccagcag cttcgggtct tcctgcggca
    24781 gcaccggcgt accggtggcc ctccgcaccg cgggcaggac ggccatcgcc tcaccgagcc
    24841 gcgcgacgcc gcgcacgacc gggacgcgcc gcagcgcctc ccgtcccggc agccgcacct
    24901 tgctccccga gacgaccctg atgccgccgc cctcctcccg cacggcggcg gcccagtagc
    24961 cgtcgctctg cagaaggact ccgtctttca aggccatacc gccgagctgc atcgtcatcg
    25021 tccccttgct ccagagcttt ccccaacact ggcgcaagcc gccgcgattc tcaagccggg
    25081 ctgagcgaga tgccgcctcg ctccacgcca caccgaggga ccgggtctgg acgcgagacc
    25141 gcgcaggcga gacacttggc accggtacac gccggcgccg tgccggcacg gcaagggagg
    25201 gcgagatggg cttcttcgac accgtcagag acaaagccgg cgccctcgcc accgacgccg
    25261 ggcgcgccgg caaagtgacc gccgcccagg cccgcctggt ggtgctgcag aacgacctgc
    25321 gcaaagcgga acgagagctc ggtcacgcgg cgttcgcgct cgccgagcgc ggggagctcg
    25381 accatcccga cctggcgcat gcggtcgagc ggctgcgggc caccgccgcc gaggtgagcg
    25441 ccaaagaggc ggagatcgcc gcacttcgcg acgaacccct cgcgccggcc gccgcgtccg
    25501 gggaggcgcc ggcccccgca gcgacggtgg cggcgacgac cgtgcagacg gccgccggcc
    25561 ctgtgaccgt cgcggcggcg gagccggagc cggtggtcgc tgccacgccc ccggcgccgg
    25621 aagccgcgcc ggaggcgccg gccgccgctc caacggaggc aggtgctcgg ccggaggccg
    25681 agacggccgt cgcggaagcg gcggcgaagc ccgcgcggcg caggaccgcc gtcaagccgc
    25741 ccatacccgt cgcgaagaag gcgccggcga agaaggcgcc gcccgcgagc aaggcgacgg
    25801 ccaggaagac ctcatcccgg gccggcgcca cgcccaagaa gtcgtcgcgg ccgcggtcgg
    25861 gaccgcggcc ggccggcaag aaccaccccg ccggcacctg acgcctgctc tgggcccatc
    25921 gcaccgtgaa cccctccgcc ggcggcccgc gtccgggccg tctggtaccc tgacggggac
    25981 caaggagacc caatgaccga ccagacctgc atccccggca ccaccgacgc acgcatcccc
    26041 atctgcgagc tcaagagtgg ccaggccgtc gagggaccgt actgctgctc agacaagatc
    26101 gccgccaccg accgcaacgg caaggcctat ctgcggctca agctgcgcga cgccagcggc
    26161 gaggtcgccg ccatccactt cgacccgtcg gacgacgcgc tcggcatcac cgtcggcgac
    26221 gtcgtcatcg tcggcggcac cttcagcgtg catcctcagt atggcgccca ggtgcaggtc
    26281 cggcgcctgc gggccgccga ccccgacgaa tacgcggcct gcgacctggt gcccgtgtcg
    26341 cccatcggcc cggccgagct tgcggagcgc ctccacgtgc ttgtcgactc cgtcgaggag
    26401 cctcatgtgc gcgcccttct cgagcgcgcg ctggacggca cccgggagcc cggagcgacc
    26461 ttcgcggtgg cgccggcggc cgtgcgcaac catcacgcgt accgccacgg actgctcgag
    26521 cactcgctca tcgtggccga ggctgcggcc ggcgtggcgt cacgcttcgc ctccgtcgac
    26581 tacgacctcg tcgtcgccgg cggcatcctg cacgacatcg gcaagaccct ggcctacacc
    26641 agcgacggca tggcgccgca gatgaccgac tccgggcgcc tgcacggcga gatcgtcatg
    26701 ggccacgaca tggtgcgcga cctcatcgac gaggaccccg acttcccgcc ggacctcgcc
    26761 ctccggctgc gccacatcat cgtgtcgcac cacggcatgc gcgagaaggg cagcccggtg
    26821 gtgccgcaga cgcgggaggc ggtcatcgtc cactactgcg acgacatgac cgcccgcatc
    26881 ggcgccatcg acgacgccga ggcggccgcc ccagccgggg atcgctggac cagccgcatc
    26941 ttcatgatcg acgcgccggc ctacgtcggc gcgcgtgaag aggccgcgga cgccgaagag
    27001 taggcgcgcc gctcagcccc agatcttcct gcagtcctcg agcacggccg ccgcgtgccc
    27061 tttgggcttc acggccggat agaccttgcg cagcgtcccg tcggcgtcga tgagccaggt
    27121 ggtgcgcttg gccacgccca ccctcggccg cgccacgcca aacgcggccg ccaccgtgtg
    27181 ctcgccgtcg tcgctgatga gcggcatctt gaggtcgtac ttccgcgccc agcgctcgtt
    27241 ggcggcgggc ttgtcgacgc tcgcgccgac cacctgcacg tccagtgagg cgaactcgtc
    27301 cagcaggccg ttgaactcaa cggcttcggt cgttcagccc ccggtgttcg ccttgggata
    27361 gaagaacagg acggtgcgtc cgcccggtgc gccgagatcg aagccggttc cgtcgtaaga
    27421 gacgccggtg accgccggcg ccttgtcgcc ttccttgagc atgtggaccc ctccgtgtga
    27481 tcggcgccgc ggccccggaa gcggagccgc acgtgccttc ggccgcctgc ttacccatcg
    27541 ggcgcgcgcc gccaaacgac gccggcgcgc ccggcccgcc tccgaggtag actccgcgca
    27601 tggcacgcaa gctgaaggtg agcgtcgact acgaccggtg cacgggggtc ggggcctgcg
    27661 agcaggtctg ccccgaggtg ttcttcatga acgacgaagg gctgccggac gtgctcgagg
    27721 agcagcccca cgagacgctc tgggccgccg tggagcgcgc cgaggaggcc tgcccagagg
    27781 aggccgtgat cctggagtgg accggggagt aggccgggcg gccttcctca gcgctcgagc
    27841 ggaaggatcg tgaccgggac acggctgcga tcgcggaacc gttcggcgtc gcggatgtcg
    27901 ccgatgatct ccccgtagtg catgggaacg gccacggccg ggccgatcag atcgcacgcc
    27961 tcggctgctt cctcccacgt catcgtgaac ttgccgccga cgggcaggaa cgcgacatcg
    28021 cagcgcacgt ccgccatctc cgggacgacg tccgtgtcgc cggcgtggta atagcggacc
    28081 ccgtcgagct cgaccacgta gccgacgcct cccgactccc gcgggtgata cggctggccg
    28141 ggggcgcgga acttgtccag gttgtaggcg tgcaccgcga gcacctccac gccggcgacg
    28201 ctcgcggtgt cgccgggagc gacggtcacg gtctcgaggc cacgcacctg accggtgacg
    28261 gaggccggac cgaccaccac ggtgtccggc cccgcgatcg cttcgatgtc ggggaggctg
    28321 aggtggtcga agtggtcgtg ggtcacgaga acgaggccgg ccggcggcga gccggacggc
    28381 agcttccagg ggtcgatgta gacggtcgcc gtcccgtcga gacggaacga atcgtggccg
    28441 agccagtgga tgttctcgag catcagtccc tcccgtgatg gctgcgtccg gcccttaccg
    28501 gaagacgaca ggacgacggg ccgggcgccg gatcccgagc gatcctccgg cggccggccc
    28561 gtcaggaact cagcgccctc tcaggcctcg gtctcgtccg cggcctcgtc ctcgtcctcg
    28621 tcgccgtcct cgtcctcgtc gccggcctcg tcctcgtcgc cggcctcggg ctcgtcgccg
    28681 gcctcaggct cgtcgccggc ctcgggctcg tcgtcggcct cgggctccgc gtcagcctcg
    28741 ggctccgcgg cggcctcagg ctcggcggcc gcggccggcg ccgccgcagc ggtcgcaggc
    28801 cggacgttcg tcgcctgcgg gcccttgcgg ccctgctcga cctcgtactc gaccagctgg
    28861 tccttgtcga tcgcggtcac gccgtcggcc aggccggtga cgtgcacgaa gatgtcgtta
    28921 tcgttctcct gctcgatgaa gccgaaggcc ttctcggtcg agaaccactt gactttaccc
    28981 tgaggcactt tgttctcctt ttgatcttga agcgcgtccc gccggtgctc ggtggtgtga
    29041 cgcgatgcgc tccttgtgcc gctcgcgccg gtctctgaga cgcagagcgt accgacagtg
    29101 tacccgcgat ccgggagacg cgaaaaccgg gcgccgtggc cttgaggcgg cgcgccgcgg
    29161 aggcccggga agccgccgcc ggtcagcagc gcgtgacggc gaagataccg atccgcgcca
    29221 gcaggttcgg agcgacgatg cgcgccggcg ggtccttgtc caccgcacgc aaatacatct
    29281 cgtgctcgat gcgaaggccc tcctcgcggc acagcttgcg gaagtcctgg acggtgcaca
    29341 ggtggatgtt gggggtgtcc caccactgat agggcaggtc gcgcgagacc ggcatgcgcc
    29401 cgcgcagaag gagctgcgag cgcggggtcc agtgggcgaa gttggggaag ctcacgatgg
    29461 cgaggcggcc gacgcgcacc atctcgcgca cgatcatgcg tggccggcgc aactgctgga
    29521 ggctcaggct caggatcacg acgtcgtggg actggtcgcc gaagtcggcg aggccggcgt
    29581 cgaggtcgcc gtggaacacc gagaggccgc ggccgatgca cttctccacc gcggcctggt
    29641 cgatctccac cccgctgccg cgcacgtgct tctcggcgat gagttcgctg aggagctcac
    29701 cgtcgccgca ggcgaggtcg agcaccgtgg cgccttccgg cacgaggtcc tcgatgagct
    29761 tgtagtcgat gcgtcgctgc accctcgcgc ctcctacctg tggtacgtcc tggccccgct
    29821 ggagaggaag gcggcgatga tgccctccat cttctcccgc tcgagcagga acgcgtcgtg
    29881 accgtagtgg ctctccacct cgtagtagct cacgtgcttg tgcgtcgcgc gcagcgcgct
    29941 gacgatctcc ttgagctggt agggcgggtg cagccagtcg ctgctgaagc ccatgaccag
    30001 gaaccgcgcc ttgacctcct cgaacgcctt gaccagggtc ccgtgctgcc gggagaggtc
    30061 gaagtagtcg agcgcgcgcg tgatgtagag gtacgtgttg gcgtcgaacc ggttggtgaa
    30121 gctcagcccc tggtggcgca ggtagctctc cacctcgaag tcggccgaga aggtgaacgt
    30181 gtagtcgagg atgtcctgca gacgccggcc gaacttctcc tccatggcct cgtcgctgag
    30241 ataggtgatg tggccgacca tgcgcgccac gctgaggccc ttggccggag tcttgccgcc
    30301 gtagtagtgg ccgccgcgcc agttggggtc ggccatgatc gcctggcggc cgacctcgtt
    30361 gaaggcgatc gcctgggtcg gctggcgcgc ggcggtcgcg agcggcacgc agaggtgggt
    30421 gcggtcgggg tgcgagacca ccagctcgag cacctgcatg ccgcccatgg acccgcccac
    30481 ggcggcgagc agcttgtcga tgccgaggtg gtcgagcagc ctcacctggg cgttcaccat
    30541 gtcggcgatg gtgatgatcg ggaagtcggt gccgaagggc ttgccggtcg ccggattcgt
    30601 cgacgcgggc ccggtgctgc cggtgcaacc gccgatgacg ttggcgcaga tgacgaagta
    30661 cttgtcggtg tccaggccct tgcccgggcc gatcatcatg tcccaccagc ccgggcgctt
    30721 gtcgttggcg ctgtgccgcc cggcgacgtg cgcgtcgccg gagagggcgt gggtgacgag
    30781 caccgcgttg tccttgcgct cgctcaagga gccgtacgtc tcgtaggcga gggtgaacgg
    30841 cgccagcgtg ccgccgccct cgagggcgag ttcctgcccc ggctggtcga acgtcacgcg
    30901 ccgggtgcgg acgatgccgg cgttgccggc gtcgtcgtcg taccccatgg cgacgtcgcc
    30961 gatctccttg cgtgctgcgg gcttgctctc ggtcatggtc ccggcgattc tatcgaaggc
    31021 tgcgacgccg ggccggtcag gccttcgcca gcgcctggtc gatgtcggcg acgatgtcgt
    31081 cgagcgtctc gatgccgacg ctgagacgca cgaagtcgtc gctcacgccg cactcgagtt
    31141 gctccgccgc cgtgagctgg gagtgggtgg tcgtggcggg gtggatcacg agggatttcg
    31201 cgtccccgat gttggccacg tgcgagagga gccgcgtgct ctcaacgaag cgccggccag
    31261 cctcgcggcc gccggcgacg ccgaacccga ccatggcccc gaacaggccg cgatagtggt
    31321 acttgcaggc gatcccgtgc gtagcgtggc actcgagtcc ggggtagccg acccaggcga
    31381 ccttggcgtg cccctgcagg tactgggcca cgccgcgggc gttgaggctg tggcgctcca
    31441 tgcgcagggg gagcgtctcc aggccctgca ggaacaggaa ggcgttgaac ggggacaggg
    31501 cggcgccgac gtcacgcagc aggctcacgc gcagcttgac gatgtacgtg atgggcccca
    31561 gcgcctcgtg gaacttgagc ccgtggtagc tcgcgtccgg ctcgacgagg ccggggaact
    31621 tgtcgttctg cgaccagtcg aaggcgccgg agtcgacgac gacgccgccg atggaggtgc
    31681 cgtggccgcc gatgaacttg gtcgccgagt ggacgacgat gtcggcgccg tgatcgaacg
    31741 gccggatgag atacggggtg gccatcgtgt tgtccacgat cagcggcaca cccgcctcat
    31801 gggcgatggc cgccacggcc tcgatgtcga gcgtgtcgag cttggggttg cccacggtct
    31861 cggcatagag cagcttggtg gtgggcttga tggcgcgacg gaagtcctcc gggtcggcag
    31921 ggtcgaccag gtcgatgtcc aggccgaagc gcggcagcgt gtggacgaag aggttgtagg
    31981 tgccgccgta caggctgttg gcgctcacga tgtggtcgcc ggcgccggcg atgttgagga
    32041 tgctgaacgt cactgcggcc gagcccgagc tgaaggcgag ggcgccgaca ccgccctcga
    32101 gcgcggcgac gcgcttctcg aacacgtcgt tggtcgggtt catcatccgc gtgtagatgt
    32161 tgccggactc gcgcaggctg aagaggtcgg cggcttggtc cgagctctcg aacgtgtacg
    32221 ccgtcgtctg gtagatgggg acggcccgcg cgttggtggc cgggtccggc tcctgacccc
    32281 cgtgcagcgc gagggtctcg aagccctggg ccttcgtatc gctgtcggtc atgtccactc
    32341 tctcctgtca gatgtagtac atgggggcct cggcggccct ggcggcggcg tcggcgatgg
    32401 tggccgcgcc gaggatggcg cgcgtgcagt cccgcacctc gcgccagacg ggggcgacca
    32461 gggcgtccgc ccggccgccg ccgcgcattc gcgccttgcc gccgttggag ccggcgcgcg
    32521 cggcggggtc cgcgcgccgg ccgtcgatga ggtcgatcac gtcggcgaag ctcaccgtcg
    32581 cggggtcgcg ggcgatgacg tagccgccgc ccttgccgcg ctggctctcc acgaggccgg
    32641 cctgacgcag gtcgtgcatc acctgctcga ggaacttggg ggggatgccg gtgaacgcga
    32701 cgatgtcgcg cgcggtgacc ggcgccgtgc cgtcggcgag cgccagccgg acgagggcct
    32761 tgatcgcgta gtcgctcttg gtggagaggt ggatcacgtc gctcccgtgc gccggataat
    32821 tcctacttct cactaggatt caggacttta ttgacgacac aggtgcttgt caaacgccgc
    32881 gccgggcctc ccccgcccgc cgacggcggg actgcgcgcc tgttacgatg tggcgcatga
    32941 ccccgccgag cgaccagacg caggccacgg ccgaccctcg cgtgcgcgag gcgctgcgac
    33001 gcgagctgcc cggcctcgaa gaggccgtcc ctctcgcgtc gatcaccgcg tacggcgtcg
    33061 gcggcccggc gcagttcctg gtgcgcgccg ggacgaccgg cgtgctggtg cacgccgtgc
    33121 tcacggcgcg cgagctggga ctgcccgtca ccgtgctcgg ccgctgcacc aacgtgctcg
    33181 tcggcgacgc cggcgtgtcc ggtctcaccg tgctggcccg caacgaaggc acgacgctgc
    33241 acggcaacgt gctgcgcgcg gaggccggcg tggagctgcc gggcctggtg gccgacctgg
    33301 cggggcagag cctcgccggg ctcaccttcg ccgccaacat cccgggctcg gtcggcggcg
    33361 ccgtggtcgg caacgccggc gcgtacggcg agtccgtgag cgacgcgctg ctcgacgtcc
    33421 acctgctcga ggagggcaag gagcacgtcg tcgggccgga ccgcctcgac ttcggctacc
    33481 gcacctcgct gctcaagtcg cggcgcgaca tcgtcgtcct ctccgcctcg ttccagctcc
    33541 accacggtca ccgccacgag ctgctgcggc gcatcgccga cgacgcggcg ctgcgcaaga
    33601 gcaagcaccc gctcgagtac gcgagctgcg gcagttactt caagaacccc tcgcgggagt
    33661 tgccggcggc ccgcctgatc gaggacgccg gcctcaaggg cctgcgcgtc ggacgcgcgc
    33721 acgtgagcga gaagcacgcc aacttcctcg tcgccgagcc gggagcgtcc gcggacgaca
    33781 tcacgacgct cgccgccgag gtcgtgcgcc gcgtcaagga gacgagcggc tacgagctga
    33841 ccgaggaggt gcggcggctc ggcttcgcct gagggccgcc gcgctatact tctcgggtcc
    33901 tgacgcgccg aacccgcccg gcagcacggg cgtctctcac ccaggaggaa ggcaatagag
    33961 gaactgccgc acggtctgat cgaccgcttg cgcagggggc agcccgaggc gcgtgccgag
    34021 ctcttccggc tctacgcgcc ccaggtgcga cgcatcctct acctccaggg gttctgcgag
    34081 gaggtcgacg acgccgtgca ggaagtgttc atcaaggtct atcgggcccg ggagatcccg
    34141 cgagaagagg cgttcctcgc gtggttctac cgcgtgatcc tcaataccgg gcgcgaccac
    34201 ggccgcaggc gcaagacgcg ctacgggctc atggacaagc tccagcagat cgcccccgag
    34261 gcgaccgtgg acccgccgtc cgagccggcc gaccccgcgc tgcgggccgc gctggcgacg
    34321 ctgccggacg agctccgcga gtgcgtcgcg ctccgcttct tcgccgacct cccgctggac
    34381 gagatcgcac gggcccagga catccccgtg ggcaccgtga agtcgcgcct gcacggcgcg
    34441 gccaagaagc tgcgcgccgc cctcgacgag caaggctacg agcgcgagtg ctgaggctac
    34501 gtacgacgat ggaagatgac cgacgatgac tgacgagcgc acagagtacg gcatgcccgc
    34561 ggcaggcggg ttcgacgatg accgcctgct ggcgtacgcg ctggggctcg aggacgaccg
    34621 ggagctgctg caggccgccg ctgccgacgc cgacctcggc gagcggctcg cggcgatgcg
    34681 cgccgacgtc gacgccatcg gcgcgcaggt ccgggccgcg gtgccggagc cggacgcgtc
    34741 gtacgcggac ctctccggcg agcgctgggc cagcctcaga gagtacctcg aggtcccggc
    34801 gagcgccgcc gcgccgcgcc gtgggcgccg ctggtggcgg gtcgtcgcgc cggtcgcggc
    34861 gctcgccgtc ctcgccctcg tcgccggcac cgtcgccctg gaccagggcg gccagcccga
    34921 ttccatcggc tcctccgccg aggtggtgcg gaccgccacg gacacgagcg attcgttcac
    34981 cgggcagagc gagagcacga cgtccggcga aggtgcggct gccgacccgt tgaccgcgcc
    35041 gggaacggag acgaccccgg gagcggcggt gaccccgccc ccggaggagg ccccgcagac
    35101 ggcagctcag agcctcgccg agcagctcga ccgcttcgcc gtcgtggtgc tcgcca
//
LOCUS       DASUMX010000189         2240 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_67396, whole genome shotgun sequence.
ACCESSION   DASUMX010000189 DASUMX010000000
VERSION     DASUMX010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67396"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="VFZ86_09505"
     CDS             <1..462
                     /locus_tag="VFZ86_09505"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5642557.1"
                     /translation="LAAAVACGKLRILDAEEIAGEVGRGAPVVAFAIRDTAELQRARD
                     TVDEVLTQAGVDDINRQRTILCISEAVTNMLLHGGGHGDMRVRRLDDRLRFVVADRGP
                     GLSFLNWIEPPAKGGQASMGYGYKIILDYLDAVALHTGPGGTTLLLDRTVH"
     gene            476..787
                     /locus_tag="VFZ86_09510"
     CDS             476..787
                     /locus_tag="VFZ86_09510"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX5642558.1"
                     /translation="MLRTSIAQGPAPTVYLAGEMDLDCADALREAVTEAARGAQMVAL
                     DFAGVEFIDSSGTGIFVRLALDLKSQGTPLVARNLSPAVRGVFDMLRVRELVGDEVFP
                     D"
     gene            complement(809..1105)
                     /locus_tag="VFZ86_09515"
     CDS             complement(809..1105)
                     /locus_tag="VFZ86_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642559.1"
                     /translation="MPPRRLLKLKHERTDLGFLGASLSEDGGRDASWMLRVESPSSFE
                     AFHDHLADGSSMALSMVTRDGEQLRGEAYVSSISDGVDTATVVVLTGAGPLRRA"
     gene            1255..>2240
                     /locus_tag="VFZ86_09520"
     CDS             1255..>2240
                     /locus_tag="VFZ86_09520"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5642560.1"
                     /translation="MTNEMGRVLVTGATGFVGLEVARRLCEHDPAPRLLVRRPLRGAL
                     LASLGAELMQGDLESPESLTRAVQGIDTVIHLGARAIFEEYELVRPTIVDGSVALMRA
                     CVAAGVRRFVYASSLLVYQGREAPVDQHTPAFTRHGYGRAKIEAERALTHIADQAGIE
                     LAILRLPHVYGVRDLMFAQVRRGLVFFPGNGRNLFAHLHVDDAARLLVAAAETGWTGI
                     SPVADDLPVNWNDFFAEIRKYYPRFRSIGTPEALALLGTSMLTPIRRLSRYPSLYTPD
                     AVRGWNVNLTVKQGLVWDDLEMTPTYPSVYQGIPAVIDDCIEFHWVHPIADRV"
BASE COUNT          325 a          824 c          770 g          321 t
ORIGIN      
        1 ctcgccgcgg ccgtggcttg cggcaagctc cgcatcctcg acgcggagga gatcgcgggc
       61 gaggtcggcc ggggggcgcc ggtcgtcgcg ttcgcgatcc gcgacacggc cgagctgcag
      121 cgggcccgcg acaccgtgga cgaggtcctc acgcaggcgg gggtcgacga catcaaccgc
      181 cagcggacca tcctctgcat ctcggaggcc gtgaccaaca tgctgctgca cggcggcggc
      241 cacggcgaca tgcgcgtccg tcgcctcgac gaccgcctgc gcttcgtcgt cgccgaccgg
      301 gggccggggc tcagcttcct caactggatc gagccgccgg cgaagggcgg ccaggcgtcc
      361 atggggtacg gctacaagat catcctcgac tacctggacg cggtcgcgct ccataccggc
      421 ccgggcggca ctacgctact attggaccgc acggtccact gacccaggag atcgcatgct
      481 gcgcacttcc atcgcccaag gcccggcgcc caccgtctac ctcgccggcg agatggacct
      541 cgactgcgcc gacgccctgc gcgaggcggt cacggaggcc gcgcgcggag cgcagatggt
      601 cgccctggac ttcgccggcg tggagttcat cgactcgtcg ggcacgggga tcttcgtgcg
      661 gctggccctc gacctcaaga gccagggcac accgctcgtc gcgcgcaacc tctccccggc
      721 cgtgcgcggc gtgttcgaca tgctgcgcgt gcgcgagctg gtcggcgacg aggtcttccc
      781 cgactgatcc gccgggaccc cggccgcgtc aggcgcgccg cagcggcccg gcgcccgtca
      841 gcaccacgac cgtcgccgtg tccaccccgt cgctgatgct cgacacgtag gcttcgccgc
      901 gcagctgctc gccgtccctg gtcaccatgc tcagcgccat ggaggagccg tcggcgaggt
      961 ggtcgtggaa cgcctcgaag ctggacggcg actccacgcg cagcatccag ctcgcgtcgc
     1021 gacccccatc ctcggacagg ctggcgccga ggaaccccag atcggtccgc tcgtgcttca
     1081 gcttgaggag tcgtcgcggc ggcatcgctg gactcatcgg ccgcggggcg cagaacctga
     1141 ggcctgccgg gtcagccggg catcgactac actaccggca gcgatgctga gcccgcgtcg
     1201 gtcgtcggtg gggacgccgg gaagcaccga ccggcgccga cgagaggcgg agtgatgacg
     1261 aacgagatgg gccgcgtgct ggtcaccggc gcgaccggct ttgtcggcct cgaggtggcg
     1321 cgccggctct gcgagcacga ccctgccccc aggctcctgg tgcgcaggcc gctccgcggc
     1381 gccttgctcg cctcgctcgg ggcggagctc atgcaggggg acctggagag cccggagagc
     1441 ctgacgcgcg ccgtccaggg catcgacacc gtgatccacc tgggggccag ggcgatcttc
     1501 gaggagtacg agctcgtgcg gccgacgatc gtcgacggct cggtcgcgct gatgcgcgcc
     1561 tgcgtcgccg ccggcgtgcg acgcttcgtg tacgccagca gcctgctcgt gtatcagggc
     1621 cgggaggccc ccgtcgacca gcacacgccg gcgttcacgc gccacggcta cggacgggcg
     1681 aagatcgagg cggagcgcgc gctgacccac atcgcggacc aagccggtat cgagctcgcg
     1741 atcctgcgcc tgccccacgt ctacggcgtc cgcgacctga tgttcgccca ggtccgccgc
     1801 ggcctggtct tcttcccggg gaacggcagg aacctcttcg cccacctgca cgtcgacgac
     1861 gccgcccggc tcctcgtcgc cgccgcggag accggctgga cgggcatcag cccggtcgcc
     1921 gacgacctcc cggtgaactg gaacgacttc ttcgccgaga tcaggaagta ctaccctcgg
     1981 ttccgcagca tcggcacgcc cgaggctctc gccctgctcg gaacctcgat gctgacgccg
     2041 atccgccgac tgagccgcta cccgtcgctc tacactcccg acgccgtccg gggatggaac
     2101 gtgaacctga cggtgaagca gggactggtg tgggacgacc tcgagatgac gccgacgtac
     2161 ccgtcggtct accaggggat ccccgcggtg atcgacgact gcatcgagtt ccactgggtg
     2221 catcccatag ccgaccgagt
//
LOCUS       DASUMX010000190        14695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_2079854, whole genome shotgun sequence.
ACCESSION   DASUMX010000190 DASUMX010000000
VERSION     DASUMX010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 14695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14695
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2079854"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="VFZ86_09525"
     CDS             <1..434
                     /locus_tag="VFZ86_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642561.1"
                     /translation="SVGFDWDSAAEAFPKIAEEHAELAELFHEAAAAEAAAAAQPESS
                     VRPPVSADPHRADPRVAHEVGDLLFATVNVARLLHVDPELALREAGNRFERRVTGAAE
                     LAAADGRDWSALDLPTQELYYQRAKGGEAPDPGEPPADAGA"
     gene            complement(525..695)
                     /locus_tag="VFZ86_09530"
     CDS             complement(525..695)
                     /locus_tag="VFZ86_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642562.1"
                     /translation="MWDEKGEIMDVPYYTCEDCGAAFTRTELLPDVEEDLLSCPECGG
                     LDIGLVEEEPAA"
     gene            810..2096
                     /gene="eno"
                     /locus_tag="VFZ86_09535"
     CDS             810..2096
                     /gene="eno"
                     /locus_tag="VFZ86_09535"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017756365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEX5642563.1"
                     /translation="MTTIIDVTARQILDSRGNPTVEVEVELESGAVGRADVPSGASTG
                     EYEAVELRDDSGEYLGKGVRTAVDNVNNVIAEEIVGFDATDQRLVDRMLIELDGTPNK
                     AKLGANAILGVSLATAYAAAAALELPLYQYVGGVNAFTLPVPMMNIMNGGKHADNNVD
                     LQEFMVMPVGAESFEEALRICAEVYHSLKGVLKKRNLSTAVGDEGGFAPDLGSNEEAI
                     QVIMEAIDAAGYEPGADARIALDPAASSFFDKEKGLYVLEGEGRDLTSAEMVDYYAAL
                     CAKYPIVSIEDGLDENDWDGFQLMTEKLGDRVQIVGDDLFVTNVERLAQGIEKRSANS
                     ILIKLNQIGTLSETLDAIQMATRAGWTAVCSHRSGETEDTTLADLAVATNAGQIKSGA
                     PARTDRVAKYNQLLRIEEMLGDVAYYPGMNAFYNLR"
     gene            complement(2234..3502)
                     /locus_tag="VFZ86_09540"
     CDS             complement(2234..3502)
                     /locus_tag="VFZ86_09540"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HEX5642564.1"
                     /translation="MRHERPLLQLVDQPYPPSACVDVAGEVARGLERAGLHAQVTRGD
                     RVAITAGSRGIRDLPAVLAAAVAAVRKAGGDPFVAPCMGSHGGASAEGQRAVLERLGV
                     TAESVGAEVVSSMDVVSVGESAFGVPVWASRDLAEADGVIVVNRVKPHTDFHGKVESG
                     LVKMLVIGAGKHAGALSAHNLTVRHGFPAVLADHGERLLAGLPVLCGIAVVEDQREQT
                     AALEVLRPDEFFAREPVLLERARELLPRLPFAQLDVLVVDRLGKDVSGAGMDTNVIGR
                     VSFWGGGDRPAEPRITRIFVRGLTPATHGNACGIGMADFTTARCAAEIDRAATGVNCI
                     TSNCPEDARIPIACATDREAISAAVQTAGVRDPGEVRLAWVRDTAHLERIAVSEALLD
                     EVRAGETLRPAGPLFPLPYDENGDLRSPFS"
     gene            3580..4353
                     /locus_tag="VFZ86_09545"
     CDS             3580..4353
                     /locus_tag="VFZ86_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX5642565.1"
                     /translation="MYCPECGNDSGNAKFCPECGADLAGVQDALGGKTGAGKVAGQGG
                     AAAPQAAAGGPKRLSPAVIWGGFGALAVVVIVIVVMMSGGFGGSAEDPAAAGASGAPA
                     QAVEADTSGSYEELVARANDLYDQGDAAFQNQDFEQGGAYFAAAAKIYAAAWAKQATD
                     PGVGTDMAVAQFYSGDIAGALKQIDAVLEAEPDFQKGWLNKGIFLSHESRISEQAGDA
                     KTAEKQLDQARVALTKAVAIDPKSDAGKQADESLQQLQQ"
     gene            4492..4941
                     /locus_tag="VFZ86_09550"
     CDS             4492..4941
                     /locus_tag="VFZ86_09550"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEX5642566.1"
                     /translation="MAQATSTAGSGPGQSRRAPSGAGRYAPRPHFGRLALLVLLVVGA
                     AFYVSPLRDFFAQQDRYEKAAAELEAARRDNAALEREVDLLATKAYIAQLARSDAMLV
                     PPDTQVFVIRGLPGKDEENRLSTATPPVQASISVLDRVEDLWRTLLH"
     gene            4978..5526
                     /locus_tag="VFZ86_09555"
     CDS             4978..5526
                     /locus_tag="VFZ86_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018838365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF501 domain-containing protein"
                     /protein_id="HEX5642567.1"
                     /translation="MSGAPPLVAGADAATVAGQLGRPPHAMSAVAARCPFGFPAVVED
                     LPYDGDGRPFPTLYYCTCPTLVAAAGRLESGGGVARWTSRLAAEPELARSAADAAADS
                     AVRRADLVEAHGLTMVDGGASLTSGVGGVSDLLAVKCLHAHVAHALARPGYVLGEAIL
                     AEVAEPWCEDRSCAAFVRGGAA"
     gene            5523..6461
                     /locus_tag="VFZ86_09560"
     CDS             5523..6461
                     /locus_tag="VFZ86_09560"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HEX5642568.1"
                     /translation="MRVAIVDLGTNTCRLFLAEVTDGRVAQAARVTTVVRLGQGVDET
                     GRLHADAVARTHACLAGYAARLEAYGPELRLLIATSVVRDALDGKQFLKAMRYEFGLP
                     WRILSGEEEGTLAFRGGTSGLGDRAPDPLLLVDIGGGSTEFAVGSAGEPPAFVRSLDV
                     GVVRLTERFFHADPPPAAQIAALADHVAAAIERGVPADHRAAVRGAVGVAGTYTTLVA
                     NKLGMTEYDARLVQGHVLSPADIDGAIARFAALTSAERGRLPGIQPGREDVILAGALI
                     AREACRAFGLDAVSVSEADLLEGAALALADGSLAPG"
     gene            6714..6803
                     /locus_tag="VFZ86_09565"
     tRNA            6714..6803
                     /locus_tag="VFZ86_09565"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6748..6750,aa:Leu,seq:taa)
     gene            complement(6827..8185)
                     /locus_tag="VFZ86_09570"
     CDS             complement(6827..8185)
                     /locus_tag="VFZ86_09570"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HEX5642569.1"
                     /translation="MKPAAEEKLGDRLISDWVSGAHLRFASDEARERYQARARLIVDT
                     IRLQDTGKIPVIPAATQKFALDYEGVTFREAMTEFDKAFAAYVRQYEDTDFDAWVGPE
                     FIFPAGMFEALAWKRVRLPGVDLPGDKSFQFVEAEYMKADEYDEYLDDPSDWVLRKYL
                     PRLSPKLEPLAQLPPLRNMLVFHQGLPDLVLAAAENPGVVEAMAALKASGEAVQEWYE
                     YLGRLDQDLGGRLGIPHLLGAIAAAPFDIISTYYRGWRGAIVDMYTRPEKMKALMERL
                     LPWQLQYGIDGAKALGHPIVAVYLYKGADGLMSDEQYEEFYWPTLKRLLLGLVEEGLL
                     VWVFTQGKYDSRLHHFKEIPAGTCLIHLESGTDALHAKEVLAGSQCIEGNVSSALLAT
                     ASVDEVEAYCLRLAEVCGEGGGFMMDFSAFLDEARRENVKKMIEVAKRYGRQETAPPP
                     LP"
     gene            complement(8185..8820)
                     /locus_tag="VFZ86_09575"
     CDS             complement(8185..8820)
                     /locus_tag="VFZ86_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008617913.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HEX5642570.1"
                     /translation="MPHELRDAIVGLRMDEALALARGMVGDGTDPLAIIAEGSEAMQA
                     IGRRYADGEAFIPELIMGGEIMRSLSAEVLPEGGGPNAGNSKGTVVIGTVQGDIHDIG
                     KDIVALMLGIDGYTVHDLGTDVPVESFVAAVREHDPDIVALSGLLTLAFGAMQSTVEG
                     IAAAGLRDQVRIMIGGAAVDENVRAYTGADGWGDDVSHAVKLAEEWTGGGA"
     gene            8949..9710
                     /locus_tag="VFZ86_09580"
     CDS             8949..9710
                     /locus_tag="VFZ86_09580"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX5642571.1"
                     /translation="MSKLNRVDRSSFEPPYAQVARSVRERIVSGEYTAGDRLPSEAEL
                     CELYGVSRMTARRAVALLVRDGVVMTENGRGTFVKAPELGAATFDLGSLRRLVDDDAT
                     SVKISEARIVPSSPRVSGKLAVGPDSPVVVIKRIVSSGGEPVFYHSEYLVFDPRRPLV
                     EAEYGVTALRDLLANAGKSGFKYGRLDLHASTLSETEASYLGEQPGVPAWVVEHLFYD
                     FENRPVSWGRFIGRADRLRFSATVGVADVPDGARR"
     gene            9707..10351
                     /locus_tag="VFZ86_09585"
     CDS             9707..10351
                     /locus_tag="VFZ86_09585"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HEX5642572.1"
                     /translation="MTSPPADDLVGLIVGMDQGGSLAVIRRRLAGDEDPRRLMEECRV
                     GMEQVGRMYERGEYFVTALIMAGEIFRQAADTLLPLIAATESPQDSRKVVIATVKGDI
                     HDIGKNIAVTLLEARGFTVVDLGVDVGAEEVADAVVAERPDVLGLSCLLTTGFEALRE
                     TIVLVRERTAGREPRVPVVIGGTALDQRAADHAGADGWCTDAADGIAVIQTLLG"
     gene            10432..10941
                     /locus_tag="VFZ86_09590"
     CDS             10432..10941
                     /locus_tag="VFZ86_09590"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX5642573.1"
                     /translation="MAETEPSVQDEYFLLWVLLAQTKDAILRARERDYARYGITNDRR
                     AVLYAVERNGGSATPVEISRNLFRELHSVTEMLKRMEADGLITREKTSGRSRIVAKIT
                     EKGRDVFTESLHNETDKRVFSVLTKRERERLRSSLMKVRGRVLQELGIPEWQLDLVLP
                     TEAADEQGS"
     gene            10938..12194
                     /locus_tag="VFZ86_09595"
     CDS             10938..12194
                     /locus_tag="VFZ86_09595"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5642574.1"
                     /translation="MTRLGELRRRPAVWRTPAGRGYILLAAMTLAFGLTMNTSNAVTT
                     NYFADVLHFTGPQFGYITAIREIPGLLLIFITALLYRMSQQRLTALALVVLAVGTAGY
                     ALATSFADVLPWVVLGSLGFHTVLQTQVALGMSLSEERHSGKVLGAMAGIGQAGALVG
                     MTAVMLLFLRWPDVYREVYVVCGFIALLGAAAIIGFPHLHEGKPIAAQMPRQRFVLKR
                     DYRFYYILNVLDGARQQLFFSFGLWVLVSRFGMGVSAVSLVVIVSTVVAMVFSPWAGR
                     MIDRHGERRTLSAINVAYIVALAGYALMGNVWLACAFYVLYSFIAPFSAIGAATYLRK
                     IAVPADIAPSLAMGVTLLHATAIVVPVAAGLILNYVGYQVPFFIGCGFALVAALVTLR
                     LDPRRQRSAARVALDEAGRAGRAAEA"
     gene            12209..12991
                     /locus_tag="VFZ86_09600"
     CDS             12209..12991
                     /locus_tag="VFZ86_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015577189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HEX5642575.1"
                     /translation="MKLDHIDGLLLDMDGVLGVSWTPLPGAAEAVTRLHAAGVPLRVL
                     TNTTALPQARFGAVLREAGFPFADEEILTASVLAATWLRAERPGARVFLIGDAQPEDL
                     AGVEMVRADEAPDVVLVSGADDSFCFATFNEVLALLRGGAELVAMHRNLFWMTRRGEK
                     LDAGAYLLGLEAATGIEAVVLGKPAPGSFRAGLDALGLPAERVAMVGDDVENDVLAAQ
                     AVGVTGVLVRTGKFREDQLARASGTPDHVVDSVLDVPGLLGL"
     gene            13139..14062
                     /locus_tag="VFZ86_09605"
     CDS             13139..14062
                     /locus_tag="VFZ86_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX5642576.1"
                     /translation="MEGWIALIVVVVVVAFIVVAVLSGIRIVRPTHRGLVERLGKYNR
                     FAGPGIHLTIPFGIERMFQVDIRETLVDATPQTIITNDNLNARVDAQVYLKVKPDEES
                     VKASQYNVANYQLQIVQLARTTLRNIIGTLSLKSANSERGRINEELRTTLRTETANWG
                     LEILRTELKEIDPPADVQETMNKVVKAENEKIAAVDYATATETQADGQRRAEIKKAEG
                     IKQAKVLAAEGEAQAIQLVNEAAERYFVGNAQVLRKLETVEKAFWQNAKIVVPADGSL
                     VNVIGDLAGIAPVPAKAAQPNPTPGQPQQRR"
     gene            complement(14113..14490)
                     /locus_tag="VFZ86_09610"
     CDS             complement(14113..14490)
                     /locus_tag="VFZ86_09610"
                     /inference="COORDINATES: protein motif:HMM:NF016854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HEX5642577.1"
                     /translation="MTAWRKPDEALVRRLDETVAALEFDRPVDYRPMFGCPAYFTGGN
                     MFAGVWEETMMLRLSEDDRTEVIAAGGAAFEPMPGRPMKEYVALPPSMVADPDTAARW
                     VHRAAAYAASLPPKKKKSRKKKT"
BASE COUNT         2132 a         5335 c         5106 g         2122 t
ORIGIN      
        1 ccagcgtcgg cttcgactgg gactcggccg ccgaggcgtt ccccaagatc gccgaggagc
       61 acgccgagct cgccgagctg ttccacgagg ccgcggccgc cgaggccgcc gcggccgcgc
      121 agcccgagtc gtcggtccgg ccgcccgtct ccgccgaccc ccaccgcgcc gaccctcgcg
      181 tggctcacga ggtcggcgac ctgctgttcg ccacggtcaa cgtcgcgcgg ctgctgcacg
      241 tggatccgga gctcgcgctg cgcgaggccg gcaaccgctt cgagcgcagg gtgacggggg
      301 cggccgagct cgccgcggcc gacggcaggg actggtcggc gctcgacctg ccgacgcagg
      361 aactgtacta ccagcgcgcc aagggcggag aggcgccgga tccgggcgag ccgcccgcgg
      421 acgccggcgc ctgaacccgg cgccggaccg gcgtctcagg ccgccggcgc ctgaacccgg
      481 caccggaccg gcgtctcagg ccgccggcgc ctcgacccgg cgtctcacgc cgccggctcc
      541 tcctcgacga gcccgatgtc gaggccgccg cactcggggc acgagagcag gtcttcctcc
      601 acgtccggga ggagctccgt cctggtgaag gctgcgccgc agtcctcgca cgtgtagtag
      661 ggaacgtcca tgatctcccc tttctcgtcc cacaccgggt atgttcccgg cgtcaggccg
      721 cgccaaatgt gtgcgaacgg tcgggggacg atccgtgtga tagcgtccca tgcgctcacg
      781 gcaagccaac gcgaggagga ccccgcgcaa tgaccaccat catcgacgtc acagcacggc
      841 agatcctcga ctcccgcggc aaccccacgg tcgaggtcga ggtcgagctc gagtccggcg
      901 ccgtcggccg cgccgacgtg ccttccggcg cgtccaccgg cgagtacgaa gcggtcgagc
      961 tccgcgacga cagtggcgag tacctcggca agggcgtgcg caccgccgtg gacaacgtca
     1021 acaacgtgat cgccgaggag atcgtcggct tcgacgccac cgaccagcgc ctggtcgacc
     1081 gcatgctgat cgagctcgac ggtacgccga acaaggccaa gctgggcgcc aacgccatcc
     1141 tcggcgtctc gctggccacc gcctacgccg ccgcggcggc gctcgagctg ccgctctacc
     1201 agtacgtcgg cggcgtcaac gccttcacgc tgccggtgcc catgatgaac atcatgaacg
     1261 gcggcaagca cgccgacaac aacgtcgacc tgcaggagtt catggtcatg ccggtcggcg
     1321 cggagagctt cgaggaggcg ctgcgcatct gcgccgaggt gtaccacagc ctcaagggcg
     1381 tgctcaagaa gcggaatctg agcacggccg tcggcgacga gggcggcttc gcgcccgatc
     1441 tcggcagtaa cgaagaggcc atccaggtga tcatggaggc catcgacgcc gcaggctacg
     1501 agcccggcgc cgacgcgcgc atcgcgctcg acccggccgc ctcgagcttc ttcgacaagg
     1561 agaagggcct ctacgtcctc gagggcgagg ggcgcgacct gacgagcgcc gagatggtcg
     1621 actactacgc ggcgctctgc gccaagtacc cgatcgtcag catcgaggac ggcctcgacg
     1681 agaacgactg ggacggcttc cagctcatga ccgagaagct cggcgaccgc gtgcagatcg
     1741 tcggcgacga cctcttcgtc accaacgtcg agcgcctcgc ccagggtatc gagaagcgct
     1801 cggccaacag catcctgatc aagctcaacc agatcggcac gctcagcgag acgctcgacg
     1861 ccatccagat ggcgacgcgc gccggctgga ccgccgtgtg ctcgcaccgc tccggcgaga
     1921 ccgaggacac cacgctcgcc gatctcgccg tggccaccaa cgccggccag atcaagagcg
     1981 gcgccccggc gcgcaccgac cgcgtcgcca agtacaacca gctgctgcgc atcgaggaga
     2041 tgctcggcga tgtggcctac tacccgggga tgaacgcctt ctacaacctg cggtaggctc
     2101 ggagcccgcg gccgcggcgg tgggcgccgg gggcacggtg tcgtgctcgc tgcgatcgag
     2161 acatgctggc tcggacgccc tgccgcgagg cccactcccg gacgcgaggc gacccggaga
     2221 agcccgagaa ggatcaggag aacggcgacc gcaggtcgcc gttctcgtcg taggggagag
     2281 ggaagagcgg gccggcgggg cgcagcgtct cgccggcccg cacctcgtcc agcagggcct
     2341 cggacacggc gatccgctcc aggtgcgccg tgtcccgcac ccaggccagc cgcacctcgc
     2401 cggggtccct gacgccggcc gtctggaccg ccgcggagat cgcctcccgg tccgtcgcgc
     2461 aggcgatggg gatgcgcgcg tcctccgggc agttggaggt gatgcagttc acgccggtcg
     2521 ccgcccggtc gatctccgcg gcgcagcggg cggtggtgaa gtcggccatg ccgatgccgc
     2581 aggcgttgcc gtgggtggcc ggcgtgaggc cgcgcacgaa gatcctcgtg atgcgcggct
     2641 cggcggggcg gtcgccgccg ccccagaacg acacccggcc gatgacgttg gtgtccatgc
     2701 cggcgccgct gacgtccttg cccagccggt ccacgacgag gacgtcgagc tgggcgaacg
     2761 gcagccgcgg cagcagctcg cgcgcgcgct cgagcagtac gggctcgcgc gcgaagaact
     2821 cgtccgggcg cagcacctcg agcgccgccg tctgctcgcg ctggtcctcc accacggcga
     2881 tcccgcacag cacgggcagg ccggcgagca gccgctcgcc gtggtcggcg agcacggcgg
     2941 ggaacccgtg gcgcaccgtg aggttgtgcg ccgagagggc gccggcgtgc ttgccggcgc
     3001 cgatcaccag catcttgacc aggccgctct cgaccttgcc gtggaagtcg gtgtgcggct
     3061 tgacgcggtt gacgacgatg acgccgtccg cctcggcgag gtcgcgggac gcccagaccg
     3121 gcacgccgaa ggcgctctcg cccacggaca ccacgtccat cgacgagacg acctcggcgc
     3181 cgacgctctc ggccgtcacg ccgaggcgct cgagcaccgc tcgctgtccc tcggccgacg
     3241 cgccgccgtg gctgcccatg cagggcgcga cgaaggggtc gccgcccgcc ttgcggacgg
     3301 cggcgaccgc ggcggccagc acggcgggaa ggtcgcggat gccccggctg ccggccgtga
     3361 tcgcgacgcg gtcgccgcgc gtcacctgtg catgcagacc cgcccgctcc agacctcggg
     3421 ccacctcccc ggcgacgtcc acgcacgcgc tcggtgggta cggctggtcc acgagttgca
     3481 gaagtggcct ctcgtgcctc acactgtctc ctcagatacc gccgaccgta tcgcggtcga
     3541 gcctaccacg acccacactg actcaaagga agcgtaagca tgtactgtcc cgaatgcggc
     3601 aacgactccg gcaacgccaa gttctgcccc gagtgcggcg cggacctggc cggcgtgcag
     3661 gacgctctgg gcggcaagac cggcgccggc aaggtcgccg gtcagggcgg cgcggccgcg
     3721 ccccaagccg ccgcgggcgg ccccaagcgc ctctcgccgg ccgtcatctg gggcggcttc
     3781 ggcgccctcg ccgtggtcgt catcgtcatc gtcgtgatga tgtccggcgg cttcggcggc
     3841 tctgcggagg accccgccgc cgccggggcc agcggcgcac ccgcgcaggc cgtggaggcc
     3901 gacaccagcg gcagctacga ggagctcgtc gcccgtgcca acgacctcta cgaccagggc
     3961 gacgccgcgt tccagaacca ggacttcgaa cagggcggcg cgtacttcgc cgccgccgcc
     4021 aagatctacg cggcggcgtg ggccaagcag gcgaccgatc cgggcgtggg caccgacatg
     4081 gcggtcgccc agttctacag cggcgacatc gccggcgcgc tcaagcagat cgacgcggtc
     4141 ctcgaggccg aacccgactt ccagaagggc tggctcaaca agggcatctt cctcagccac
     4201 gagtcccgga tcagcgagca ggccggcgac gcgaagaccg ccgagaagca gctcgaccag
     4261 gccagggtcg cgctcaccaa ggccgtggcg atcgatccca agtcagatgc cggcaagcag
     4321 gccgacgaga gcctgcagca gctccagcag tagacgcgca cacagacgcg ggacccccag
     4381 ggttcgcggc cgccgggcag ggacacacgc cggcggccgc gaacccttag ggcgagaagg
     4441 ggccgtcctg cgcagtgcgg gcggcacatg gcgagccggg agcaggagag cttggcccag
     4501 gcgacctcaa cagcgggctc cggaccgggg cagtcgcggc gcgcgccgtc gggcgcgggc
     4561 cggtacgcac cgcgtcccca cttcgggcgt ctggcgctgc tcgtgctgct ggtcgtcggc
     4621 gccgcgttct acgtctcgcc gctgcgcgac ttcttcgcgc agcaggaccg ctacgagaag
     4681 gccgcggccg agctcgaggc ggcgcgccgg gacaacgcgg cgctcgagcg cgaggtcgac
     4741 ctcctcgcca ccaaggccta catcgcccag ctcgcgcgct cggacgcgat gctcgtgcct
     4801 cccgacaccc aggtcttcgt gatcaggggg ttgccgggca aggacgagga gaaccgcctc
     4861 agcacggcga cgccgcccgt ccaggcctcc atctccgtcc tcgaccgcgt cgaggacctg
     4921 tggcgcaccc tgctgcactg aggcgccggt gacccgcgct gccgcggcga ggcccgcatg
     4981 agcggggcgc cgccgctggt ggccggcgcg gacgccgcta ccgtcgccgg ccagctgggc
     5041 cgcccgcctc acgcgatgag cgccgtcgcg gcgcgctgcc cgttcgggtt cccggccgtc
     5101 gtcgaggacc tgccctacga cggcgacggc cggcccttcc cgacgctgta ctactgcacg
     5161 tgcccgacac tggtcgccgc cgccggcagg ctcgagagcg gcggcggcgt ggcgcgctgg
     5221 acctcccggc tcgccgccga gcccgagctc gcgcgctcgg cggcggacgc cgccgccgac
     5281 tccgccgtcc gccgcgcaga ccttgtcgag gcgcacgggc tcaccatggt ggacggcggc
     5341 gcctcgctca ccagcggcgt cggcggcgtg agcgacctgc tcgccgtgaa gtgcctgcac
     5401 gcgcacgtgg cgcacgcgct cgcgcggccg ggctacgtgc tcggcgaggc gatcctcgcc
     5461 gaggtcgccg agccgtggtg cgaggaccga agctgcgccg ccttcgtgcg cggtggtgcg
     5521 gcgtgagggt cgccatcgtc gatctgggca ccaacacctg ccggctgttc ctcgccgagg
     5581 tcacggacgg acgcgtggcg caggcggcgc gcgtgacgac ggtcgtgcgg ctgggccagg
     5641 gtgtcgacga gaccgggcgc ctgcacgcgg acgccgtggc gcggacccac gcctgcctcg
     5701 cggggtacgc tgcacgcctc gaggcctacg ggcccgagct gcgcctcctc atcgccacca
     5761 gcgtcgtgcg cgacgccctc gacggcaagc agttcctcaa ggccatgagg tacgagttcg
     5821 gcctgccgtg gcggatcctc agtggggagg aggaggggac actcgccttc cgcggcggga
     5881 cctcggggct gggcgatcgc gcgccagacc ccctgctgct cgtcgacatc ggcggcggca
     5941 gcaccgagtt cgccgtcggc tccgccggcg agccgccggc gttcgtgcgc agcctggacg
     6001 tcggcgtggt gcggctcacg gagcgcttct tccatgccga cccgccgccg gcggcgcaga
     6061 tcgcggccct cgcggaccac gtcgccgccg ccatcgagcg cggcgtgccg gcggaccacc
     6121 gcgccgcggt ccgcggcgcg gtcggcgtcg ccggcaccta cacgacgctc gtcgccaaca
     6181 aactcggcat gaccgagtac gatgcgcggc tcgtgcaggg ccacgtactc tcgccggccg
     6241 acatcgacgg cgcgatcgcc cgcttcgccg cgctgacgag cgcggagcgc ggccggctcc
     6301 cgggcatcca gcccggccgc gaggacgtca tcctcgccgg cgcgctcatc gcccgcgagg
     6361 cctgccgcgc gttcggcctc gacgccgtca gcgtcagcga ggccgacctc ctcgagggcg
     6421 ccgcgctcgc cctcgccgac ggctcgctgg cgccgggttg accgtcccag cgacggcctg
     6481 tagcttgcgg caaagcacgc cgtccgcccc gggaggtccc tcgtgccgct gcgccgcctg
     6541 acgcgtcgct acgtcgtcgt cgctccgctg ctcaccttcg ccgcgtggct cttcgtcacc
     6601 ggcgccttcg gcccggccgc ctgaccgacg gcgggcgtcc ggtagccgcg gcgcttcacg
     6661 ccgcactccg tcgcacgctc cgcctgcccg cacccgtcat atactgcacg cgtgccggag
     6721 tggcggaact ggtagacgcc ggggacttaa aatcctctgt cgccgctggc ggcgtccggg
     6781 ttcgaacccc ggctccggca ccacgtgccc gccacgcccc catctcctac ggcagagggg
     6841 gaggtgcggt ctcctggcgc ccatagcgct tcgcgacctc gatcatcttc ttgacgttct
     6901 cccgccgggc ctcgtcgagg aaggccgaga agtccatcat gaagccgccg ccttcgccgc
     6961 agacctcggc gagccggagg cagtaggcct cgacctcgtc gacggacgcc gtggccagga
     7021 gcgcgctcga gacgttgccc tcgatgcact gcgaccccgc gagcacctcc ttcgcatgaa
     7081 gagcgtcggt gccgctctcg aggtggatga ggcacgtccc cgccgggatc tccttgaagt
     7141 ggtgcaggcg cgagtcgtac ttgccttgcg tgaagaccca caccagcagg ccctcttcca
     7201 ccagcccgag gaggagccgc ttgagcgtcg gccagtagaa ctcctcgtac tgctcgtcgg
     7261 acatgagtcc gtcggctccc ttgtacaggt acacggcgac gatcggatgt ccgagagctt
     7321 tcgctccgtc gatgccgtac tgcagctgcc aaggcaggag gcgctccatg agggccttca
     7381 tcttctccgg ccgcgtgtac atgtcgacga tggcgccgcg ccagccgcgg tagtaggtgc
     7441 tgatgatgtc gaacggcgcc gcggcgatgg cgccgagcag gtgcgggatg cccagccggc
     7501 cgccgaggtc ctggtcgagg cgcccgaggt actcgtacca ctcctgcacg gcctcgcctg
     7561 acgccttgag cgcggccatg gcctcgacga cgccggggtt ctcggctgcg gccaggacga
     7621 ggtcgggcag gccctggtgg aagacgagca tgttgcggag cggcggcagc tgcgccagcg
     7681 gctcgagctt gggcgacagc cgcggcaggt acttgcgcag cacccagtcc gaggggtcgt
     7741 ccaggtactc gtcgtactca tcggccttca tgtactcggc ttccacgaac tggaacgact
     7801 tgtcgccggg caggtcgacc cccggcaggc gcacgcgctt ccaggccagc gcctcgaaca
     7861 tgcccgccgg gaagatgaac tcgggcccga cccaggcgtc gaagtccgtg tcttcgtact
     7921 gacgcacgta ggcggcgaac gccttgtcga actcggtcat cgcctccctg aacgtgacgc
     7981 cctcgtagtc gagggcgaac ttctgcgttg cggcggggat gaccgggatc ttgccggtgt
     8041 cctggagccg gatggtgtcg acgatgagcc gggctctggc ctggtacctc tccctggcct
     8101 cgtcgctcgc gaaccgcagg tgcgcgccgc tcacccagtc actgatgagc cggtcgccga
     8161 gcttctcctc tgctgcaggc ttcatcatgc gcccccgccg gtccactctt cggcgagctt
     8221 gacggcgtga ctcacgtcat cgccccagcc gtcggctccc gtgtacgcgc gcacgttctc
     8281 gtcgactgcg gcgccgccga tcatgatcct gacctggtca cggagccccg cggccgcgat
     8341 gccctcgacg gtactctgca tggcgccgaa ggccaacgtg agcagcccgc tcagcgcgac
     8401 gatgtccggg tcgtgctccc tcacggcggc gacgaagctc tcgaccggca catcggtgcc
     8461 gaggtcgtgg acggtgtacc cgtcgatccc gagcatgagc gcgacgatgt ccttgccgat
     8521 gtcgtggatg tcgccctgga ccgtgccgat gacgaccgtg cctttggagt tccccgcgtt
     8581 cggtccacct ccctcgggaa gcacttccgc cgagaggctc ctcatgatct cgccgcccat
     8641 gatcagctcg gggatgaacg cctcgccgtc ggcgtagcgg cggccgatcg cctgcatcgc
     8701 ctcgctgccc tctgcgatga tggcgagcgg gtccgtcccg tcgcccacca tgccccttgc
     8761 gagtgccagc gcctcgtcca tgcgcagacc cacgatcgcg tccctcaact cgtgcggcat
     8821 gcgtcctcct ccatctgctc aacgggggaa tcactctagc agatgcccga atacctgtct
     8881 agacaggtat ggagccatgg tacaatgcag acgcgcgagc cagcgtggag tctccggccg
     8941 gagggtgcgt gagcaagctc aaccgcgtcg atcgttcatc gttcgaaccc ccgtacgccc
     9001 aagtggcgcg cagcgtgcgg gagcgcatcg tcagcggcga gtacacggcc ggcgaccgcc
     9061 tgccctccga ggccgagctc tgcgagctgt acggggtgag tcgcatgacg gcgcgccggg
     9121 cggtggcgct cctggtgcgc gacggcgtcg tcatgaccga gaacggacgc gggacgttcg
     9181 tcaaggctcc ggagctcggt gccgcgacgt tcgacctcgg aagcctgcgc cgcctcgtgg
     9241 acgacgacgc tacgtcggtg aagatcagcg aggcgcggat cgtgccgtcc tcgccgcggg
     9301 tgagcggcaa gctcgccgtg ggccccgact cccccgtggt cgtcatcaaa cgcatcgtca
     9361 gcagtggcgg ggagccggtc ttctaccaca gcgagtacct cgtcttcgat ccgcggcgtc
     9421 cgctcgtcga ggccgagtac ggggtgaccg cgctgcgcga cctgctggcg aacgcgggca
     9481 agtcgggctt caagtacggt cgtctcgacc tgcacgcctc cacgctctcg gagaccgagg
     9541 cgtcctacct cggcgaacag ccgggcgtgc cggcgtgggt ggtcgagcac ctcttctacg
     9601 atttcgagaa ccggccggtg agttggggcc ggttcatcgg ccgggccgac cgcctcaggt
     9661 tctcggccac ggtcggtgtc gccgacgttc cggatggggc gcgccggtga catctccgcc
     9721 tgcagacgat cttgtcggcc tgatcgtagg gatggaccag ggcggcagcc tcgccgtcat
     9781 ccgccggcgg ctcgccggcg acgaggaccc acgcaggctg atggaggagt gccgcgtggg
     9841 gatggagcag gtcggcagga tgtacgagcg cggcgagtac ttcgtcaccg cgctcatcat
     9901 ggctggggag atcttccgcc aggccgcgga caccctgctt cccctgatcg cggccacgga
     9961 atctcctcag gactcgcgca aggtggtcat cgcgacggtc aagggcgaca tccacgacat
    10021 cggcaagaac atcgcagtga cgctgctcga ggcccggggc ttcacggtcg tcgacctcgg
    10081 cgtcgacgtc ggcgcagaag aggtcgcgga tgccgtcgtg gcggagcgtc cggacgtgct
    10141 cggactctcg tgcctgctga ccacgggatt cgaggccttg cgcgagacga tcgtgctggt
    10201 gcgcgaacgc actgccggac gggagccgcg cgtgccggtc gtcatcggcg gcacggccct
    10261 cgaccagcgc gccgcggacc acgcaggcgc cgacggctgg tgtacggacg ccgccgacgg
    10321 catcgccgtc atccagaccc tgctgggctg atccacagac gcacggcccg accggccgat
    10381 gctaggatgg tcacaccctg catgagcggc gcttcgaggc tgggagtcga gatggcggag
    10441 accgaaccgt cggtccagga tgagtacttc ttgctctggg tgctgctggc ccagaccaag
    10501 gacgccatct tgcgggcgcg ggagcgcgac tacgcgcggt acggcatcac caacgaccgg
    10561 cgcgcggtcc tctacgccgt cgagcgcaac ggcggcagcg ccaccccggt cgagatctcc
    10621 cgcaacctct tccgcgagct ccactccgtc accgagatgc tcaagaggat ggaggccgac
    10681 ggcctcatca cgagagagaa gacgtccgga cgctcgcgga tcgtggcgaa gatcacggag
    10741 aagggacgcg acgtcttcac cgagtccctg cacaacgaga ccgacaagcg cgtgttttcc
    10801 gtcttgacga agagagaacg tgagcgtctg aggtcgtcgc tcatgaaggt ccgcgggcgc
    10861 gtccttcagg aactcggcat ccccgagtgg cagctcgacc tcgtgctccc caccgaagcg
    10921 gcggacgagc agggcagttg acccggctcg gtgagctccg gcgccggccg gcggtgtggc
    10981 ggacgcccgc cgggcgcggc tacatcctcc tcgccgccat gacgctggcg ttcggcctca
    11041 cgatgaacac cagcaacgcg gtgaccacca actacttcgc cgacgtgctg cacttcacgg
    11101 ggccgcagtt cggctacatc acggccatcc gggagatccc ggggctcctg ctgatcttca
    11161 tcacggcgct gctgtaccgc atgtcgcagc agcgcctcac ggcgctcgct ctggtcgtcc
    11221 tcgccgtggg cacggccggc tacgcgctgg cgacgagctt cgcggacgtg ctgccgtggg
    11281 tcgtgctcgg cagcctcggg ttccacaccg tcctccagac gcaggtcgcg ctcggcatga
    11341 gcctctccga ggagcgtcac agcggcaagg tgctgggcgc catggccggc atcgggcagg
    11401 cgggggctct cgtggggatg acggcggtga tgctcctctt tctgcgctgg ccggacgtct
    11461 accgcgaggt gtacgtggtc tgcgggttca tcgccctgct cggggccgcc gccatcatcg
    11521 gcttcccgca cctgcacgag ggaaagccga tcgcggcgca gatgccgcgc cagcgcttcg
    11581 tcctgaagcg cgactaccgc ttctactaca tcctcaacgt gctggacggc gcccggcagc
    11641 agctgttctt ctcgttcggc ctgtgggtgc tggtgagccg cttcgggatg ggcgtctcgg
    11701 cggtctcgct ggtggtcatc gtctcgaccg tggtggccat ggtgttctcg ccctgggccg
    11761 ggcgcatgat cgaccgccac ggtgagcgcc gcacgctctc ggcgatcaac gtcgcctaca
    11821 tcgtcgcgct cgccggctac gcgctcatgg gcaacgtgtg gctggcgtgc gccttctacg
    11881 tgctgtacag cttcatcgcg ccgttctcgg cgatcggcgc ggcgacgtac ctgcgcaaga
    11941 tcgctgttcc ggcggacatc gcgcccagcc tggccatggg cgtgaccctg ctgcacgcca
    12001 cggccatcgt ggtgcccgtc gcggccggtc tcatcctcaa ctacgtcggc taccaggtgc
    12061 cgttcttcat cggctgcggc ttcgcgctcg tggcggcgct ggtcacgctg cggctggacc
    12121 cacggcggca gcgctccgcc gcccgggtgg cgctcgacga ggccgggcgc gctggccggg
    12181 ccgccgaggc gtgagatagg ctcaggcgat gaagctggac cacatcgacg gcctgctgct
    12241 ggacatggac ggcgtgctcg gcgtctcgtg gacgccgctg ccgggcgccg cggaggccgt
    12301 cacgcgcctg cacgccgccg gcgtgcccct gcgcgtgctc accaacacca cggcgctgcc
    12361 acaggcgcgg ttcggcgccg tgctccgcga agcggggttc ccgttcgccg acgaggagat
    12421 cctcacggcg tcggtgctgg ccgccacgtg gctgcgcgcg gagcgtccgg gcgcgcgcgt
    12481 cttcctcatc ggggacgcgc agccggagga cctcgccggg gtcgagatgg tccgggcgga
    12541 cgaggcgccg gacgtcgtcc tcgtctccgg cgccgacgac tcgttctgct tcgcgacctt
    12601 caacgaggtc ctggccctgc tgcgcggcgg cgcggagctc gtcgccatgc accgcaacct
    12661 gttctggatg acccggcgcg gcgagaagct ggacgccggg gcctacctcc tcggcctcga
    12721 ggccgccacg ggcatcgagg ccgtcgtcct cggcaagccg gcgcccgggt cgttccgcgc
    12781 cggcctggac gcgctcggcc tgccggccga gcgcgtcgcc atggtcggcg acgacgtgga
    12841 gaacgacgtc ctcgccgccc aggccgtggg cgtcacgggc gtgctcgtgc gcacgggcaa
    12901 gttccgcgaa gaccagctgg cccgtgcctc cggcacgccc gaccacgtcg tcgactccgt
    12961 gctcgacgtt cccgggctgc tcgggctcta gtctgcccgg cctccggccg cggcccctgt
    13021 cgtgccttcg gccgcgtgag tccttgccat tgccggggaa caaccctagc atcggctgtg
    13081 aacttcatcg tgccccgggc ggccgccggc cgctcctcgc gcacggaaag gggtcgtcat
    13141 ggagggttgg atagcgctca tcgtcgtcgt cgtggtggtg gcgttcatcg tggtcgccgt
    13201 tctgtcgggc atccgcatcg tgcgccccac gcaccgcggt ctggtggagc gcctcggcaa
    13261 gtacaaccgg ttcgcggggc cgggcatcca cctcaccatc ccgttcggca tcgagcgcat
    13321 gttccaggtg gacatccgcg agaccctggt cgacgcgacg ccgcagacca tcatcaccaa
    13381 cgacaacctc aacgcccgcg tcgacgcgca ggtctacctc aaggtcaagc cggacgagga
    13441 gagcgtcaag gcgtcgcagt acaacgtcgc caactaccag ctgcagatcg tgcagctggc
    13501 gcggaccacc ctgcgcaaca tcatcggcac gctgagcctc aagtcggcca acagcgagcg
    13561 cggccgcatc aacgaggagc tgcgcaccac gctccgcacc gagaccgcca actgggggct
    13621 cgagatcctg cgcacggagc tcaaggagat cgacccgccg gcggacgtgc aggagacgat
    13681 gaacaaggtg gtcaaggcgg agaacgagaa gatcgccgcc gtcgactacg ccaccgccac
    13741 cgagacgcag gccgacggcc agcgccgcgc cgagatcaag aaggccgagg gcatcaagca
    13801 ggccaaggtc ctcgccgccg agggcgaggc gcaggccatc cagctggtca acgaagcggc
    13861 cgagaggtac ttcgtcggca acgcccaggt gctgcgcaag ctcgagacgg tggagaaggc
    13921 gttctggcag aacgccaaga tcgtcgtccc ggcggacggc agcctggtga acgtgatcgg
    13981 cgacctcgcg ggcatcgcgc cggtgccggc caaggcggcg cagcccaacc cgacgcccgg
    14041 ccagccgcag cagcggcgct gagcgcccgt cggcggagac gagcggcgta ggcgcggccc
    14101 tcgcggccgc gcctaggtct tcttcttgcg cgacttcttc ttcttcggcg gcagcgacgc
    14161 ggcgtacgcc gccgccctgt gcacccaccg ggccgccgta tccgggtcgg cgaccatgga
    14221 cggcggcagg gcgacgtact ccttcatcgg gcgccccggc atgggctcga acgccgcgcc
    14281 gcccgcggct atgacctctg tccggtcgtc ctcggagagc cgcagcatca tcgtctcctc
    14341 ccatacgccg gcgaacatgt tgccgccggt gaagtacgcc gggcagccga acatgggccg
    14401 gtagtccacc ggccggtcga actcgagcgc cgcgaccgtc tcgtccaggc gccggacgag
    14461 tgcctcgtcg ggtttgcgcc atgcggtcac cgcgtgtgct cctcgtcttc cgccgaatcc
    14521 ggccccggct acttcttctc gtggcgcggt acgacgagca cggggatgtc gctgtggtgc
    14581 agcagcttga gggcggtgga cccgaggaac gcggcggaca tcgcgcccga gccgtggctg
    14641 ccgacgacga tggccacggc cttcttctcg cgggcctgct cgagcagcac gtcga
//
LOCUS       DASUMX010000191         4363 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1649143, whole genome shotgun sequence.
ACCESSION   DASUMX010000191 DASUMX010000000
VERSION     DASUMX010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4363
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1649143"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="VFZ86_09615"
     CDS             <1..378
                     /locus_tag="VFZ86_09615"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX5642578.1"
                     /translation="VSIVGFATGGTVDGIVLVVYFVAYQQFENNLLQNVVYSKTVSLS
                     PFIVFLAALIGASLGGIVGVLLAIPLTSAGWVLAGDLLALRRERAERRGGRKPLVTDP
                     PPEDGPGASLGSRPDEEPAAEDD"
     gene            complement(388..2553)
                     /locus_tag="VFZ86_09620"
     CDS             complement(388..2553)
                     /locus_tag="VFZ86_09620"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX5642579.1"
                     /translation="MTARDAMTAPVACNRDCGGGCPLLATVDGGRVVRIADNPAGGPY
                     LRGCVRGFQAWRQQQAGDRLTHPLVRTGPRGSGRFREAGWEEAVTLVADGLARVRERH
                     GDASILALGGSGSCRGALHDTEDLTARYLNLSGGHVEQTSTYSSAASSYTQPVVLGTQ
                     YAGVDPATLRHSSMIVLWGANLVDCIMGCEWRARVREAKRRGVPVVVVDPRRTTTARL
                     LGTEWLPVLPGTDSALMLALLHELIAGGAVDERFVAAHATGYDALRRRVLGLDPGEGA
                     ADVAPPAAGGTAAGPPRGAPATPEWAAGVCGVPAARIRALARSWASHRPVALVPGLSF
                     QRTLGGEEAVRLAIALQTVTGDLGRPGGSSGGRTWGGLPKPRVGSIPVPPNPAAAGIP
                     CNDWAGAVLHGRAGGHSADVKAAYDTGGNYVVQGADVALNIRAMLALEFSVCHDLFLT
                     TTARYCDVVLPATHWLERSDIVFTSADYVLYSQRVVAAPGLARDDYDIFADLAEASGC
                     GTAFSEGRDSAAWLDHFLEGSEVDDPEEFRRTGVHWGADRERVGLAEFSADPVGAPLA
                     TPSGRVELAGAACAAAGLSEVPEARVLEPEPESPLSFISPKSRLRVHTQFAALPWVRE
                     RDDRTLWINPADAAPRGVADGDDVLVTNERGRLRCACHVTGDVMPGVVSLLAGIEPSF
                     DEDGCDVAGSANVLTSPEPTLPSRGSRLLSTLVEVRRAG"
     gene            complement(2720..3667)
                     /locus_tag="VFZ86_09625"
     CDS             complement(2720..3667)
                     /locus_tag="VFZ86_09625"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEX5642580.1"
                     /translation="MAELLVYCERIDTARELVAGGRELAAALGLGVSAVALGPGAGAA
                     ASELAAAGADTVYVSEDAAFEGWPADAVAAGLAQVAGLAGATVVLVGSTRRGKETAPR
                     LAQKLGAGCVTDVSALVAEGGDLVAARYAFGGATVAREKVTTPVKVFAVMPKTFSDEG
                     AVAGAGAVQTPTLNVESRVRVVDRRAKEGDHVNLDAAPLIVGVGRGFNSREDLALGED
                     LAEALGAVVGCTKSLADFEWLGEDRIIGLSGAKTAPDLYLGVGISGQIQHTVGVAGAK
                     LIAAVNKDKEAPIFAMADYGLVGDLYEVVPALVARLKDV"
     gene            complement(3670..>4363)
                     /locus_tag="VFZ86_09630"
     CDS             complement(3670..>4363)
                     /locus_tag="VFZ86_09630"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="electron transfer flavoprotein beta subunit/FixA
                     family protein"
                     /protein_id="HEX5642581.1"
                     /translation="VPYKIGDLEKNALQAAVDLRDAVGGKVVALCLAEGNRKAAEDIK
                     VALAMGADEAAVVEDPALAAPDQAAVAAALAAAVEKLGACDLLLFGEGSSDNYTGQVG
                     ARVAELLGLPQIGYARKVEATEGGVRCERSMDDMIETLETTLPAAVTVVSEINEPTIP
                     GLMQIMKAKSKPLQVWSLADLGIEAPAAVQETVSNLAEEQDRKHVLFEGTAAEQADAF
                     VDALIREGVLGR"
BASE COUNT          550 a         1664 c         1577 g          572 t
ORIGIN      
        1 gtctcgatcg tcgggttcgc gaccggcggg accgtcgacg gcatcgtgct ggtcgtctac
       61 ttcgttgcct accagcagtt cgagaacaac ctgctgcaga acgtcgtcta ctcgaagacg
      121 gtctcgctct ccccgttcat cgtcttcctg gccgccctca tcggggcaag cctcggcggg
      181 atcgtcggcg tgctgctcgc gatcccgctc acctccgccg gctgggtgct cgccggcgac
      241 ctcctcgcgc tgcgccgcga gcgggccgag cggcgtggcg gccgcaagcc gctcgtcacc
      301 gacccgccgc ccgaagacgg accgggcgcg agcctcggct ccaggcccga cgaggagccc
      361 gccgccgagg acgactgaag cgcggtctca gccggcgcgc cgcacctcga cgagcgtcga
      421 gagcaggcgg gagccgcggc tggggagcgt cggctcgggg ctggtgagca cgttggcgga
      481 cccggcgacg tcgcaaccgt cctcgtcgaa gctcggttcg atgccggcga gcagcgagac
      541 gacccccggc atgacgtcgc cggtgacgtg gcaggcacag cgcagccggc cgcgctcgtt
      601 cgtcaccagc acgtcgtcgc cgtcggcgac tccccggggc gcggcgtcgg cggggttgat
      661 ccacagggtc cggtcgtcgc gctcgcgcac ccacggcagc gcggcgaact gcgtgtgcac
      721 gcgcagccgc gacttgggcg agatgaagga gagcggcgac tccggctccg gctcgagcac
      781 ccgcgcctcc gggacctcgg agagcccggc cgccgcgcag gccgccccgg ccagctccac
      841 gcgccccgac ggcgtggcga gaggggctcc gaccgggtcg gcggagaact ccgcgagccc
      901 cacgcgctcg cggtccgcac cccagtggac gccggtgcgc cggaactcct cgggatcgtc
      961 gacctcggag ccctccagga agtggtcgag ccacgccgcc gagtcccggc cctcgctgaa
     1021 cgccgtgccg cacccgctcg cctcggccag gtcggcgaag atgtcgtagt cgtccctggc
     1081 cagcccgggc gcggctacca cgcgctgcga gtacagcacg tagtcggctg aggtgaacac
     1141 gatgtcactg cgctccagcc agtgcgtggc cgggagcacg acgtcgcagt agcgggccgt
     1201 cgtcgtcagg aagaggtcgt ggcagacgga gaactcgagc gcgagcatgg cgcggatgtt
     1261 cagggcgacg tccgcgccct gcacgacgta gttgccgccg gtgtcgtagg cggccttgac
     1321 gtccgcggag tggccgccgg cgcggccgtg cagcaccgcg ccggcccagt cgttgcaggg
     1381 gatgccggcg gccgcggggt ttggcgggac cgggatgctg cccacgcgag gcttggggag
     1441 tccgccccag gtgcggccgc cgctcgaccc gcccgggcgg ccgaggtcgc cggtcacggt
     1501 ctgcagggcg atcgcgaggc gcacggcctc ctcgccgccc agcgtccgct ggaaggagag
     1561 cccggggacg agcgcgaccg gccggtggct cgcccaggag cgcgcgagcg cgcggatgcg
     1621 cgctgccggc acgccgcaca cgccggcggc ccattccgga gtcgccggcg ccccgcgggg
     1681 cggtccggcc gcggttccac cggccgccgg cggcgcgacg tccgcggcgc cctcgccggg
     1741 atcgagcccg agaacgcggc ggcgcagggc gtcgtagccg gtggcgtgcg cagcgacgaa
     1801 gcgctcgtcg acggcgcccc cggcgatgag ctcgtgcagc agcgccagca tgagggcgct
     1861 gtcggtgccc gggagcaccg gcagccactc cgtaccgagc agcctcgccg tggtggtgcg
     1921 ccgcgggtcg acgaccacga ccggcacgcc ccgccgcttc gcctcgcgca cccgtgcccg
     1981 ccactcgcat cccatgatgc agtccaccag gttggctccc cagagcacga tcatggacga
     2041 gtgacggagc gtcgccggat cgacgccggc gtactgcgtg cccagcacca ccggctgggt
     2101 gtagctggag gccgccgagc tgtacgtcga tgtctgctcc acgtgcccgc cgctgaggtt
     2161 gaggtagcgg gcggtgaggt cctcggtgtc gtgcagcgcg ccgcggcacg accccgaccc
     2221 ccccagcgcc aggatcgagg cgtcgccgtg ccgttcgcgc acgcgcgcga gaccgtcggc
     2281 gacgagcgtg accgcctcct cccatccggc ctcgcgaaag cggcccgagc cccgggggcc
     2341 ggtgcgcacc agcgggtgcg tcaggcggtc cccagcctgc tgctgccgcc acgcctggaa
     2401 cccgcgcacg cagccccgga gatacgggcc gcccgccggg ttgtcggcga tgcgcaccac
     2461 gcggccgccg tccacggtcg cgagcaacgg gcagccgccg ccgcaatcac ggttgcaggc
     2521 gaccggcgcc gtcatcgcgt cgcgagccgt catcgtccgt cccctctggt cgtcgtggtc
     2581 accctgagga taccggacgc aaagagggcc ggggcgccgc gaggcggccc cggcccgggc
     2641 gctccggcgg agccgtctca caccgccgaa gcagccgtgg tgtgacgccg ggggcggcgg
     2701 acggcgccgc ccggccgcgt cacacgtcct tgaggcgcgc gacgagcgcc ggcaccacct
     2761 cgtagaggtc gccgaccagg ccgtagtcgg ccatggcgaa gatcggcgcc tccttgtcct
     2821 tgttcacggc ggcgatcagc ttggcgccgg ccacgccgac ggtgtgctgg atctggccgg
     2881 agatgcccac gccgaggtac aggtccggag ccgtcttggc gccggagagg ccgatgatgc
     2941 ggtcctcgcc gagccactcg aaatcggcga ggctcttggt gcagccgacg acggcgccca
     3001 gcgcctccgc gagatcctca cccagggcga ggtcctcgcg ggagttgaag ccgcgcccca
     3061 cgccgacgat gagcggcgcg gcgtcgaggt tgacgtggtc gccctctttg gctcgcctgt
     3121 cgacaaccct caccctcgac tcgacgttga gggtcggcgt ctggacggct cctgcgccgg
     3181 ccaccgcgcc ctcgtcgctg aacgtcttgg gcatgacggc gaacaccttg accggggtcg
     3241 tgaccttctc tcgcgccacg gtggcgccgc cgaacgcata ccgcgcggcg accaggtcgc
     3301 cgccctcggc gacgagcgcg ctcacgtcgg tgacgcaccc cgcgccgagc ttctgcgcca
     3361 ggcgcggcgc cgtctccttg ccgcgccgcg tggatcccac gagcacgacg gtcgcgccgg
     3421 cgaggccggc gacctgggcg aggccggcgg ccacggcgtc ggccggccag ccctcgaagg
     3481 cggcgtcctc gctcacgtac acggtgtcgg cgccggcggc ggcgagctcg ctcgccgcgg
     3541 ccccggcgcc cggccccagc gccacggcgc tcacgccgag gccgagggcc gccgccagct
     3601 ctcttccccc cgcgaccagc tcgcgggcgg tgtcgatcct ctcgcagtag accagcaact
     3661 cggccatcgt tacctcccca ggacgccttc gcggatcagg gcgtcgacga aggcgtcggc
     3721 ctgctcggcc gcggtgccct cgaacaggac gtgcttgcgg tcctgctcct ccgccaggtt
     3781 gctcaccgtc tcctgcaccg ccgccggcgc ctcgatgccg aggtcggcca gcgaccacac
     3841 ctgcagcggc ttgctcttcg ccttcatgat ctgcatgagg cccgggatcg tcggctcgtt
     3901 gatctcgctg accacggtca cggccgccgg cagcgtcgtc tccagggtct cgatcatgtc
     3961 gtccatgctg cgctcgcagc ggacgccgcc ctccgtcgcc tcgaccttgc gcgcgtagcc
     4021 gatctgcggc aggccgagca gttcggcgac acgcgcgccg acctggccgg tgtagttgtc
     4081 gctcgagccc tcgccgaaca gcagcaggtc gcaggcgccg agcttctcga cggcggccgc
     4141 gagagccgcg gccacggccg cctggtccgg cgccgccagc gccggatctt cgacgaccgc
     4201 ggcctcgtcg gcgcccatgg cgagcgcgac cttgatgtcc tcggcggcct tgcggttgcc
     4261 ctcggcgagg cacagggcga ccaccttgcc gcccacggcg tcgcgcaggt ccaccgccgc
     4321 ctggagggcg ttcttctcga ggtcgccgat cttgtacggc acg
//
LOCUS       DASUMX010000192         3902 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_643836, whole genome shotgun sequence.
ACCESSION   DASUMX010000192 DASUMX010000000
VERSION     DASUMX010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3902
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_643836"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..984)
                     /locus_tag="VFZ86_09635"
     CDS             complement(<1..984)
                     /locus_tag="VFZ86_09635"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HEX5642582.1"
                     /translation="MPRAKLTVWGDAECRRLHEATLKLLADAGVDVRHEPGLELFQAA
                     GATVEGQRVRIPARLVEEALASAPREWVIKPRGGDTAPLILSNGQVHYGTGSDVLYVR
                     DPETGERRRVRRADVEGMAALCEQLPQIDFVMSMGLPEDAPQEVDDLVQVAAMLRGTR
                     KPLLVAPRDGSVLARMQEMAELCGEKESLAIYAMPSPPLMHDPDALTKVIGCAELLIP
                     LVYAPAPNMGATGPRSIAGAVLVGNAETLSGLVLHQHVNRGAPFVYGVGAGAMDMRTM
                     TDPYVSPDALLAQQVGTDVARHYGLPSFSYAGMSDSKLLDEQWTAEAALSMD"
     gene            1221..2264
                     /locus_tag="VFZ86_09640"
     CDS             1221..2264
                     /locus_tag="VFZ86_09640"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEX5642583.1"
                     /translation="MFPPIGDESACLHWLWTALYAPDGVSTVCRHCRVMRRFHRVSRR
                     RAYACDHCGTQVYPTAGTFMQGSGLGVATWFAAAWQMMQGDGNVAPRALADELGVSYK
                     TALRIKRKLEEALGPGGADADLLERLRRDAGGPGALAAGVPARDRSSRARENIRAAAC
                     RAFAARGPSATRIADIAREAGVSSAIIHYYYKSKDEVLLAALQWANEHTSRQLVELHR
                     QTTDPIELLRRTIDLAIPSEGVLRDEYVLWIETWARARLHPRLLAECAAMSEGWTRFF
                     QQLVEDGTAAGVFHPIAPADEIAQRMVALSDGLSFRSAIGYRGMQVHQVRELLVSFAA
                     EQLGVPAAALAEP"
     gene            complement(2292..2930)
                     /locus_tag="VFZ86_09645"
     CDS             complement(2292..2930)
                     /locus_tag="VFZ86_09645"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX5642584.1"
                     /translation="MAATAAERAYELLKDRIITTRMKPGAMIEEAVLVSDLGLGRTPV
                     REALKRLEAERLVVVLPRRGMYVAEVTLAELRELEEVRLSLETTSARLAVQRITPGQL
                     AELEDLLAESADGPDGGVSAGERSLEMDRRLHELIWRASDNSLLEVECGRLFNLSMRM
                     WYMLVDRLESSDLHEEVFAEFLAAARNKDEQRAEDAVRNHILLFGDSIRRHL"
     gene            complement(3117..>3902)
                     /locus_tag="VFZ86_09650"
     CDS             complement(3117..>3902)
                     /locus_tag="VFZ86_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012663107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HEX5642585.1"
                     /translation="WFFVLLLGPTLFLINLTIGATGQYILNFLPMSLYTGIGDDTNWL
                     GSWTIFYWAWWMAWAPFVAVFIARISKGRSIRQLVAFTLILPTIADLLWYGVIGGAGI
                     KYDITEELNANGLESAIFATAQNLPLTSILVVVIFVLIVAFFLTAADSASMSLAMFTS
                     TEEDPNKYLRLFWAIALGAVAAVLAATGSLQVVQTASIVVAFPMAFLFFPVIYGVFRG
                     LSQSIRESAVEVAAAPAGQPETAAAGEALTAGAATVVQARTDA"
BASE COUNT          622 a         1360 c         1335 g          585 t
ORIGIN      
        1 gtccatgctg agtgcggcct cggcagtcca ctgctcgtcg aggagcttgg agtcggacat
       61 gccggcgtag gagaagctcg gcaagccgta gtggcgagcc acgtcggtgc cgacctgctg
      121 ggcgagcagc gcgtccggcg agacgtaggg atcggtcatg gtgcgcatgt ccatcgcacc
      181 ggcgccgacg ccgtagacga acggcgcgcc cctgttgacg tgctggtgga gcaccaggcc
      241 gctgagagtc tcggcgtttc caacgagcac cgcgccggcg atgctccggg gtcccgtcgc
      301 ccccatgttg ggcgccggtg cgtagaccag cgggatgagc agctccgcgc agccgatgac
      361 cttggtcagc gcgtcagggt cgtgcatgag ggggggcgac ggcatcgcgt agatggccaa
      421 gctctccttc tcgccgcaga gctccgccat ctcctgcatg cgcgcgagca cggagccgtc
      481 gcgcggcgcc acgagcagcg gcttgcgcgt gccgcggagc atcgccgcga cttggacgag
      541 atcgtcgacc tcctgcgggg catcctcagg caggcccatg ctcatgacga agtcgatctg
      601 cggcaactgc tcgcagagcg ccgccatgcc ctcgacgtcg gcgcggcgga cccggcggcg
      661 ctcaccggtc tccgggtccc tcacgtacag cacgtcggag ccggtgccgt agtggacctg
      721 cccgttgctc aggatgagcg gcgcggtgtc gccgccgcgc ggtttgatga cccactcgcg
      781 cggcgcgctc gcaagcgcct cctcgacgag gcgcgcgggg atacggacgc gctggccctc
      841 gaccgtcgcg ccggcggcct ggaacagctc caggccgggc tcgtggcgca cgtccacgcc
      901 tgcgtcggcc agcagtttca aggtggcctc gtgcagacgc cggcactcgg cgtcgcccca
      961 gacggtgagt ttcgccctcg gcattccgat cctcccgacc gtgtcgtggg agcagacagc
     1021 gctggcgccg ctcgctccgt acggttcgcc tgacttcgac atcaagtcat ttcacgctca
     1081 aagtggaatg tcaagcacgt atacggtcga ggcatgattc acaagttcgc ctgaaccgtg
     1141 taccatgcgc caagtaaggc ggcgatgagg ggtggacggt ggatcaggag acactcgcac
     1201 ttacggcgac gggagcgagg atgttcccgc cgatcgggga cgagtcggcc tgcctgcact
     1261 ggctctggac ggcgctgtac gccccggacg gcgtcagcac ggtctgccgc cattgccgcg
     1321 tgatgcgtcg cttccaccgt gtgtcgcgac gccgcgctta cgcctgcgac cactgcggca
     1381 cccaggtgta tcccaccgcc ggcaccttca tgcagggctc gggtctgggc gtcgccacct
     1441 ggttcgccgc cgcctggcag atgatgcagg gcgacggcaa cgtcgcgccg cgcgcactcg
     1501 ccgacgagct cggcgtgagc tacaagaccg cgctgcgcat caagcgcaag ctcgaggagg
     1561 cgctgggccc gggcggcgcc gacgcggacc tgctggagcg gctgcgccgc gacgccggtg
     1621 gacccggggc gctcgccgcc ggcgtccccg cgcgcgaccg gtcctctcgg gcgcgcgaga
     1681 acatccgcgc cgcggcctgc cgggctttcg cggcgcgcgg cccgtcggcg acgcgcatcg
     1741 ccgacatcgc gcgcgaggcg ggcgtctcga gcgcgatcat ccactactac tacaagtcca
     1801 aagacgaggt gctgctcgcc gccctgcaat gggccaacga gcacacaagc cggcaactgg
     1861 tcgagcttca ccgccagaca acggacccga tcgagctgct acgtcggacc atcgacctcg
     1921 cgatcccctc cgagggggtg ctgcgcgacg agtacgtgct ctggatcgag acctgggcgc
     1981 gggcacgcct gcatccgcgc ctgctcgccg agtgcgccgc gatgtcggag ggctggacca
     2041 ggttcttcca gcagttggtg gaggacggga ctgcggccgg agtgttccac cccatcgccc
     2101 cggccgacga aatcgcccag cgcatggtcg ccctctcgga cggcctcagc ttccgctcgg
     2161 cgataggcta ccgaggcatg caggtgcacc aggtccgcga gctgctcgtg agcttcgccg
     2221 ccgagcagct cggcgtgccg gcagccgcgc tcgccgagcc ctgacgccag gttcgcacca
     2281 ccgccggtga ctcagaggtg acggcggatc gaatccccga agagcaggat gtggttgcgg
     2341 accgcatctt cagcccgttg ctcgtccttg ttcctggcgg ccgccaagaa ctcggcgaag
     2401 acctcttcgt gtagatcgga ggactcgagc cggtccacga gcatgtacca catgcgcatc
     2461 gagaggttga agagcctgcc gcactcgacc tcgagcaggg agttgtcgct cgcccgccag
     2521 atgagttcgt gcagccgcct gtccatctcg agactgcgct cgccggcgga gactccaccg
     2581 tccggaccat cggcggactc ggcgagcagg tcctccagct ccgcgagttg ccccggggtg
     2641 atgcgctgca cggcgaggcg ggcgctcgtc gtttccagag agagccgcac ctcttccaac
     2701 tcccgcagtt ccgcgagggt gacctcggcc acgtacatgc cacgcctcgg cagaaccacg
     2761 accagccgct ccgcctcgag acgcttgagc gcctcccgga cgggagttcg gcccaacccg
     2821 aggtcgctga ccaggaccgc ctcttcgatc attgcgcccg gcttcatgcg cgtggtgatg
     2881 atcctgtcct tgagcagctc gtacgcccgc tctgcggcag tcgcggccat gtcgaatcct
     2941 tctcggtcct gggtctccct gtgccgtggc gggaccggcg ccctgccacg gcgctcacga
     3001 ttctcctatc ggacgccccg cgcacgcaac aggcgggtcg cccgcgaagg cggcccccgg
     3061 tcccgacccg ccgggccaag agggagaggc ccggcgggtc ggagcggagc gcgcggtcag
     3121 gcgtccgtgc gcgcctggac gacggtggca gcgccggccg tgagcgcctc acccgcggct
     3181 gcggtctcgg gctgccctgc gggcgccgcg gcgacttcga cggccgactc ccgaatcgac
     3241 tggctaaggc cgcggaacac gccgtagatc accgggaaga acaggaacgc catcgggaag
     3301 gccacgacga tcgacgccgt ctgcaccacc tgcaagctgc cagtcgcagc cagcaccgcc
     3361 gccacggcac ccagagcgat ggcccagaac aggcggaggt acttgttcgg gtcctcttcc
     3421 gtcgaggtga acatcgccag cgacatcgac gccgagtcgg cggcagtcag gaagaaggcc
     3481 acgatcagca cgaagatcac gaccaccagg atgctggtga gaggcaggtt ctgggcggtg
     3541 gcgaagatcg cgctctccag gccgttggcg ttgagctcct cggtgatgtc gtacttgatg
     3601 ccggcgccgc cgatcacgcc gtaccacaag aggtcggcga tcgtcgggag gatcagcgtg
     3661 aacgccacga gctggcggat ggagcggccc ttggagatgc gggcgatgaa cacggccacg
     3721 aacggcgccc aagccatcca ccacgcccag tagaagatcg tccagctgcc gagccagttg
     3781 gtgtcgtcgc cgatccccgt gtacaggctc atcggcagga agttcaggat gtactgcccc
     3841 gtggcgccga tcgtgagatt gatcaggaac agcgtcggac ccagcagcag cacgaagaac
     3901 ca
//
LOCUS       DASUMX010000193         2229 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_930630, whole genome shotgun sequence.
ACCESSION   DASUMX010000193 DASUMX010000000
VERSION     DASUMX010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_930630"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..495
                     /locus_tag="VFZ86_09655"
     CDS             118..495
                     /locus_tag="VFZ86_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642586.1"
                     /translation="MNTHIDIDQGRLRVTLHGLDIVWTTRRRMIVPLAHVVGARLDPY
                     IHRRGPWLGAGRTDALLGYAVAAGSMVVHGRHEFWDVHHPEKAIVIDLVDEAYDRLVL
                     EVDDPDALVVEINAAVATSEAAA"
     gene            complement(505..1461)
                     /gene="speB"
                     /locus_tag="VFZ86_09660"
     CDS             complement(505..1461)
                     /gene="speB"
                     /locus_tag="VFZ86_09660"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEX5642587.1"
                     /translation="MTYDSLPFSGIATFFKAPLVPMPTTADADVAVVGIGWDEGTTSR
                     SGARMGPRALREASTLYAFQRGAEPFWDGEAGVELLGGIRWADTGDVALGPMWSPERY
                     HNAVIDKLQPIMSSGLFPVTLGGDHSIGYPVLKALYQARGGKPVHLVQFDTHMDYWEE
                     EGGSRFSHASPIIRSHEAGFIAGLTQYGIRSLHTPGDNIALAQSRGAHIFWCQQAKDA
                     LVDDLVEHLPKGGDVYITFDIDALDPAIAPGTGTPEPGGFSYYEAKEILLAVCARCNV
                     VGFDVVEVAPQYDGPGQVTSLHGARLILDTVGAVFRRRATLG"
     gene            complement(1458..>2229)
                     /locus_tag="VFZ86_09665"
     CDS             complement(1458..>2229)
                     /locus_tag="VFZ86_09665"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5642588.1"
                     /translation="DIACLGYIEEMILRYKATPDSIAGVNVEFIQGENGIQIPPPEWP
                     GRLSALCKKYGWILYNDEVQEGMGRCGEWFLVDAYPDVEVELLSLGKATSGGLIPICY
                     TLGSDRMSEPAGELYTGGTFAGSPVGCMVGIKLIEIIERDNVLANVKEIEKIAKAKFG
                     AMRDKYEIVGDVRVKGCYQCVEFVEDKDSKTPAHDLTREVVYAMERRGVIPIYEPAFN
                     WFRPTPALNMPPELFALGCDIVEECVAEVSKAHAKGIA"
BASE COUNT          323 a          762 c          780 g          364 t
ORIGIN      
        1 ccagcgtcgt cggccagggc tccgtgttca ccgtgctgct gccggcgtga cgccgtaggg
       61 gcgggcgggc tcccgcgccg ccggtcagcc gctcgccccg acgggaatac tccgggcatg
      121 aacacccaca tcgacatcga ccaggggcga ctgagggtga cgctacacgg actcgacatc
      181 gtctggacca cccgcaggcg catgatcgtg ccgctcgcgc acgtcgtggg cgcgcggctg
      241 gacccgtaca tccaccgtcg cggcccctgg ctcggggcgg gccgcaccga cgcgctgctc
      301 ggctacgccg tcgcggccgg ttcgatggtg gtgcacggcc gccacgagtt ctgggacgtc
      361 catcatcccg agaaggcgat cgtgatcgat ctcgtggacg aggcgtacga ccgcctggtg
      421 ctcgaggtgg acgatcccga tgcgctcgtc gtggagatca acgccgccgt cgcaacgagc
      481 gaggcggcgg cgtagtccct gccgctaccc cagtgtcgcg cgcctccgga agacggcgcc
      541 cacggtgtcg aggatcagcc gtgcgccgtg cagcgacgtg acctgaccgg ggccgtcgta
      601 ctgcggcgcc acctccacga cgtcgaagcc gaccacgttg cagcgggcgc acacggcgag
      661 caggatctcc ttggcctcgt agtagctgaa gccgccgggc tcgggcgtcc cggtgcccgg
      721 cgcgatggcc gggtcgaggg cgtcgatgtc gaaggtgatg tagacgtcgc cgcccttggg
      781 caggtgctcg accaggtcgt cgaccagcgc gtccttggcc tgctggcacc agaagatgtg
      841 cgccccgcgg ctctgggcca gggcgatgtt gtcgccgggg gtgtgcaggc tgcggatgcc
      901 gtactgcgtg aggcccgcga tgaagcccgc ctcgtggctg cggatgatgg ggctggcgtg
      961 cgagaagcgc gagccgccct cctcttccca ataatccatg tgcgtgtcga actggaccag
     1021 gtgcaccggc ttgccgcccc tggcctggta cagggccttg agcaccgggt agccgatgga
     1081 gtggtcgccg ccgagcgtga cggggaagag gcccgagctc atgatcggct gcagcttgtc
     1141 gatcaccgcg ttgtggtagc gctcgggcga ccacatgggg ccgagcgcga cgtcgccggt
     1201 gtccgcccag cggatgccgc cgagcagctc cacgccggcc tcgccgtccc agaacggctc
     1261 ggcgccgcgc tggaaggcgt agagagtgga ggcctcgcgc aaggcgcgag gccccatgcg
     1321 cgcgcccgag cggctggtgg tgccctcgtc ccagccgatg ccgacgacgg ccacgtcggc
     1381 gtcggccgtc gtcggcatcg gcaccagcgg cgccttgaag aaggtcgcga tgccgctgaa
     1441 gggcagtgaa tcgtaggtca tgcgatgcct ttcgcgtgcg ctttgctcac ctcggccacg
     1501 cattcttcca cgatgtcgca gccgagcgcg aacagctcgg gcggcatgtt gagcgccggc
     1561 gtgggccgga accagttgaa cgccggctcg tagatcggga tcacgccccg ccgctccatg
     1621 gcgtagacga cctcgcgcgt gaggtcgtgc gccggcgtct tgctgtcctt gtcctcgacg
     1681 aactcgacgc actggtagca gcccttgacg cgcacgtcgc cgacgatctc gtacttgtcc
     1741 ctcatggcac cgaacttggc cttggcgatc ttctcgatct ccttgacgtt ggccagcacg
     1801 ttgtcgcgct cgatgatctc gatgagcttg atgccgacca tgcagcccac gggggagccg
     1861 gcgaaggtgc ctcccgtgta gagctcgccg gccggctcgc tcatgcggtc cgaccccagc
     1921 gtgtagcaga tggggatgag cccgccggac gtggccttgc cgagcgagag caactcgacc
     1981 tccacgtccg ggtacgcgtc caccaggaac cactcgccgc agcggcccat gccctcctgg
     2041 acctcgtcgt tgtagaggat ccagccgtac ttcttgcaga gcgccgacag acgccccggc
     2101 cactccggcg gcgggatctg gatgccgttc tcgccctgga tgaactccac gttgacgccg
     2161 gcgatgctgt cgggcgtggc cttgtagcgc aggatcatct cctcgatgta gccgaggcag
     2221 gcgatgtcg
//
LOCUS       DASUMX010000194        14853 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1363046, whole genome shotgun sequence.
ACCESSION   DASUMX010000194 DASUMX010000000
VERSION     DASUMX010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 14853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14853
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1363046"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            199..786
                     /locus_tag="VFZ86_09670"
     CDS             199..786
                     /locus_tag="VFZ86_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642589.1"
                     /translation="MIWGGFGVAAVVIVLIVVFVTTGGGGEPTGAAPTGGTQSVQPVA
                     ADTSGSYGELVERGNGLYDQGAAAFESEQWDQGAAYFAAAAKVYAAAWKLQSTDPGVG
                     TDFATSLFYSGQIEPAIAQVEQVLAQTPTFQTGWFNKGNYLAEKARIAEQDGSAKVAK
                     AAYADARAAYEKAVALGADSASGQQAQQRMDALPN"
     gene            821..2239
                     /locus_tag="VFZ86_09675"
     CDS             821..2239
                     /locus_tag="VFZ86_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015607138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 domain-containing protein"
                     /protein_id="HEX5642590.1"
                     /translation="MVELLPEITQARKLTPRERAALGKAARKRAPRSAHARWEVSAGR
                     PDPVGLLEEQAADRVSDLVPIRYGRMLVSPGTFYRGGALLMATDLAGTPDSGLRAQLC
                     GDAHLLNFGLFQSPERRLVFDINDFDETLPGPWEWDVKRLAASFEIAARDRGFPAATR
                     RSIVLHSVRAYREAMHEMAEKRAIQLWYTRLDADLIREHVTVVGARDARRMARDMSRA
                     ETKDDLRALSRLTCEVEGRTRLRSAPPLLVPVRELLEGDERARFTHSVEQALRAYRET
                     LPRDRRAAYDLYRYRDMARKVVGVGSVGTRAWVLLFFGRDEGDPLFLQAKQAQASVLE
                     RFVGRSRFRGAGRRVVEGQRLMQAVGDILLGWYSVTGFDGRPYDFYVRQLWDGKGTFN
                     VTAMTEGAWHTYAEMCSWVLARAHSRSGDRIAIAAYLGGADVFDRAVADFADAYAEQN
                     QRDYDALRAAVDSGRVSAQGGV"
     gene            2290..3855
                     /locus_tag="VFZ86_09680"
     CDS             2290..3855
                     /locus_tag="VFZ86_09680"
                     /inference="COORDINATES: protein motif:HMM:NF021619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PocR ligand-binding domain-containing protein"
                     /protein_id="HEX5642591.1"
                     /translation="MEAEGYELASLIDLDRVQRLCESLSQAFDFALAVLDRSGNVLIA
                     TGWQDICTRFHRENADTLSGCLESDLRINRRLAQGLDPSEHYAYRCRNGLWDVAFPLV
                     VAGEHVANVYTGQFLFDDDDVDRAEFARRARRLGFDEQAYLAALDRVPVISHERLQKT
                     VRFLADFVGMLGEMGVAALQERRKHAQLVESEERYHRLFDNATEGLTVFRVERGPGGE
                     VEDVVVVDLNPIQAERTRVTRAQLIGRRLSECDGHDERLRAYYDVVRGAAEARCATRS
                     EACLHARDAYELLSAYPAGDEDLWVLSATDVTDLREAERALHRQDEAIRAAYVDVLDA
                     VTGGKLILLTEEQLPEELGRSLGRQFAFGAPSELAAARGMIVRAAEACFPGRIRRNDL
                     LSTAGEALDNAVKHAGGGTFQAFCRGESLQVAITDEGPGIDFRTLPRATLVPGFSTAA
                     SLGMGFTIMLQLCERVLLSTRPGRTVVVLEIAAAGEPARDQSARGPAPADGSGLTSRS
                     AVTSESGVSPNVA"
     gene            complement(3759..4139)
                     /locus_tag="VFZ86_09685"
     CDS             complement(3759..4139)
                     /locus_tag="VFZ86_09685"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5642592.1"
                     /translation="MRVLLATDRPDLGHALTLFLSERSIQVVDVVDDVGSLMDRAAAS
                     RPDVVLVDWCLGDAVSSRMVADLMDRDDPTPVIVLTTAQDRSRAHACGAAAYATFGDT
                     PDSLVTALREVSPDPSAGAGPRAD"
     gene            complement(4273..4905)
                     /locus_tag="VFZ86_09690"
     CDS             complement(4273..4905)
                     /locus_tag="VFZ86_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015788095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5642593.1"
                     /translation="MSDHDDIRVLIVDDHQMVREGLKVLLSTYADIDVVGEAANGLEA
                     QEQCRLLDPHVVLMDVRMPVMDGAEATAEIMANCPGVRVIALTSYIDQESVEKTLDAG
                     AISYLLKDARPEALTQAIRDAVEGRGTIDSGVMRALMERRHDNVGGNLTAREREVLAL
                     LAGGLSNKEIAERLILSVGTVRLHVSNILSKLGAPNRTTAAAIAMKHGLT"
     gene            complement(4902..6932)
                     /locus_tag="VFZ86_09695"
     CDS             complement(4902..6932)
                     /locus_tag="VFZ86_09695"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEX5642594.1"
                     /translation="MNLPEVLMGVAAGIAILAALTHGLVGFSTRPPDRAGIAFAIAAA
                     AAAAGALSVLALYTIHDIDLHIAVMKWAYFPATVVWTAAVVWFVAFVADVRPMRFLLA
                     LTAGFVFTLVVNVVLPRGILHEQRGGLMEMDAMGGSVMVMTESSPHILQNVTDVLTLI
                     SFGFLCYAVYRVSRRPAQTRARYLGLMTALLAVATLFDAVIEHRVVITFNTLYLSQVS
                     FAAVIIAGSLVLRRESLRVEMELQLYRTHMDELVEARVRELDEAHERLAEESRERRAT
                     EEVLRRRVEELDVLTRMAQILAGRTTLGQALHEATSATSGLFKARYVRVRLLTDGEAA
                     LEAAACLDAAAPPAAGDGRAAGRDGIQALSDLDLAVTDRVMADCEMVAEDVADWPGLP
                     ETVRRQARADGVGPVLAEPLVASSGPAGALVIARDAGASPFTEEERQLARTIGEALAA
                     VIEIDRLYHRAAEEAAEEERQALARDLHDAVTQSIYSATLIAEALPAVWERDPAEGAH
                     NLERLRRLVRAAMAEMRTLLFELRPAALESAPLAALLERLGDALGGQTQVAVDIEADE
                     TIDLPPDVKIVFYRVAQEAFNNVAKHARASSVTARVSAVDGGASLTVADDGRGFDPHE
                     IAGGMGLSIMRERLRRIAGTLEVDTTPGGGTTIRAAWPSTAYDEGAAERMGA"
     gene            complement(6949..10260)
                     /locus_tag="VFZ86_09700"
     CDS             complement(6949..10260)
                     /locus_tag="VFZ86_09700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642595.1"
                     /translation="MRRRGRGGPREGDLDGHRPARLTAALAALTAAVTLAAGLAACSG
                     DEAAGPQAGVAPRGASAVDAGVTNGATTYLARDLIAIMADAPSQDARFAAASLAAQKD
                     AAGPAAPEPRLDPGATPDYFGIANWANSPRLRKFVDRLPGVGPDAANALGQYIPVAVP
                     DTVTYPGADYYELAVREYSEQLHSDLPRTRLRGYVQLNQGTDASGRNTVRPAGIHYLG
                     PLIRARRGRPVRIKFVNQLAPGAAGDLFLPVDTTTRGTGTGPDGGEDVYPQNRASLHL
                     HGGLTPWISGGSQYQWITPAGEKSPYRSGPSLVNVPDMWFDARGVPVAEGAPGATNDP
                     GPGATTLYFPNDQSARFLCLHDDTYGLTRLSVYAGEAAPYLLGDEVEDELVETGTVPA
                     EELPLVIEDKTFVPAAERLRTTDPTWDIARWGGLGALWYPHVYMPNQNGVGEEGAVSD
                     QPTINAKGRWDYLPWYWTGYTGTENGPVANPLFGTVPSQPEENPGTPSPSVVPNAFHD
                     TMLVNGTAYPYLEVERKAYRLRILNACGDRQLNLQLYYATSNEIDETAADGSPELQTA
                     SGDVSMLPAQPSMSGGWPARWPTDTRVGGVPDPKAAGPAMIQIANDGGLLPRAVTHKP
                     TPVGLENRLPTDQEASMGEYASIVAVTTKALYLAPGERADVIVDFSKVPAGSKLILYN
                     DAPAPAPNGDGRVDYYTGDRDKTAIGGAPATESGYGPSTRTVMQFQVDGPAAPPFDVA
                     RLQRALPAAYAAAQDPVLVPTAAYRDVYGDALEPDADGQDPAAPDVPEEESGTLTFTP
                     LERVYRLTLPVQGKMVSDLFDPLFGRKTGTLGVDAPLSAIGIRTAVPYSAIDPATEYL
                     ALDGEATAPQIGDATQIWRVTHDGSLSHSVTFTGFAVQVLARARREGETRAPDPAELG
                     WKDTLRVDPLESVLIAMRPVLPELPFKLPASSRALDVTLPLGAEGAFTQLDPVTAAPL
                     ARPVVNAPAELGFEAYWGIHLQGGEEHHTVRPLVLQGTTTAPSGVTAAAAGAAVELAW
                     TAPLFPPPVTGYVVERAADEGFSSDVQAFTAAEDATTYTDATAQSGSTYFYRVRAESA
                     AGWSAWSAAAEVSVP"
     gene            10352..10870
                     /locus_tag="VFZ86_09705"
     CDS             10352..10870
                     /locus_tag="VFZ86_09705"
                     /inference="COORDINATES: protein motif:HMM:NF025745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4418 family protein"
                     /protein_id="HEX5642596.1"
                     /translation="MKVLAVLIIIMGLVIIIVPQYTNCEAGKDQPATLNMQTSDAGVI
                     EVASTVAMEGTMESAAVPYRMMKCLWSARAEIVAGVPLIALGALLLFARRKETIRVIG
                     IVTTLLGVLTILIPTSVIGVCMNPEMVCNTQMKPTLLIAGGITVALGIAVLVLGEMRR
                     PTDGPAEAAAAA"
     gene            10899..12086
                     /locus_tag="VFZ86_09710"
     CDS             10899..12086
                     /locus_tag="VFZ86_09710"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX5642597.1"
                     /translation="MIRLARSGISSNPLRSWLVGGCALLVAAMVLTTVVISRGGQESL
                     RLARERLGADVVVVPRGSVSGVEGALLLGNATNTYMPRGVVDKVRSVPGVAQASPQLY
                     LTSMANSSCCSVSLMFMFAFDPVTDFTIEPWLQEKLGQDLQEGQAVGGSDVFVPEGED
                     KIKLYGYDFDLVANLERTGTNLDQTLFMTYPTAYAMQQVSQTEAKMALPVLSDTVSSV
                     LVKVADGADRSEVAMAIGEAVPEVDAVPSVQMFSSYGDQISGVLRTLIVIVVLTVILS
                     LTLMAVIFWMSVNERSRQIGVLRALGATQRFVLLAYMTEAALLALAGGLAGVVLAAVV
                     VYFFHDLLVSALGFTILLPSAPSLIGLVVVGMLGALAVAAAAAFVPALRASRQEPAVA
                     MRE"
     gene            12092..12796
                     /locus_tag="VFZ86_09715"
     CDS             12092..12796
                     /locus_tag="VFZ86_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011194988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5642598.1"
                     /translation="MTAVSLRGVGKAYGDKDAATFAALRDVDLDVETGEFLILTGRSG
                     CGKTTLLNIASGLATPTSGSVKVEGVDLWSISDAERSNLRNRTMGFVFQFPSLLPGLT
                     LAQNVTLPLEFSAEEHADAAGRARELLETVGLGHRADALPRELSAGQQQRVVVARSLI
                     NRPTLLLADEPSSDLDQDTEAEIMEIFSRIHREQGITIIMATHARNLISFGTRHLEMA
                     DGTLRPDGVAAAGNGD"
     gene            13040..>14853
                     /locus_tag="VFZ86_09720"
     CDS             13040..>14853
                     /locus_tag="VFZ86_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020490331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642599.1"
                     /translation="MAARVARHMSLARASILVFIVVALLTALFMVKMSQGEAMPTGPT
                     DSTTVPHYFGPWPNWALSPLTLPDATVTIDGDGTGAAAVATVGGNGVITGIHVTNPGS
                     GYTTATVGITGAGTGAAASATITSTGAIVAVTVGNPGSGYKAPVVTISGGGATADATA
                     TAYGGVDAITLQNAGTGYQFPTVDFDMPDDPNGIKAEAAATIDANGAITGITITNPGS
                     GYATAPTVVIRDGTLMDPVANAGAGASAVATINVTTVHLLTFGAGYTSAPTVTITDSV
                     GTGSGATATAAVNNGLISAIDVTAAGSGYVTGGGIRKFQDQLPMLCNPAVPGSCEDAA
                     AANNLGKFLPLGVPEEKTYPDPDGNPILSDEYEIGLIQYRSKFNTDLPATLARAYVQI
                     DTSADAATTGYPLENELLDGTKVPVMIDGLQAYGVTSPQWLGPVIAATKNKPVRIVFR
                     NLLPTGSDGDLFLPTDSTLMGSGMGPMGLADPVDEGSVMDGVRNPVCSEAPKSPDCFK
                     DNRATLHLHGGISPWISDGTPHQWITPANEDTPWPEGVDVRYVPDMPVGNDPRDGVMT
                     FYYTNQQSARLMFYHDHAWGTTRLNVYAGEAAGYIISD"
BASE COUNT         2094 a         5320 c         5322 g         2117 t
ORIGIN      
        1 ttctgtcccg attgcggcgc gcctacgggc gcgcccgccg cggctgccac cccggtggca
       61 acggccgccg gccggcgtac cggcacgcgc ggccgaagcc cgggagccgc gcgccggcag
      121 cagcgccctg ccaggcaggc ggtcaagaag ccggcgcctg agactaccgc cggctctccg
      181 cgccgtctgt ccccggcggt gatctggggc gggttcgggg tcgccgccgt cgtgatcgtc
      241 ctcatcgtcg tcttcgtgac gacaggtggc ggcggggagc cgaccggggc cgccccgacg
      301 ggcgggacgc aaagcgtcca gccggtcgcg gccgacacca gcggcagcta cggagagctg
      361 gtggagcgcg gcaacggcct gtacgaccag ggggcggcgg ccttcgagtc agagcagtgg
      421 gaccagggcg cggcctactt cgccgctgcc gccaaggtct acgccgcggc gtggaagctg
      481 cagagcaccg acccgggcgt cggcaccgac ttcgcgacct cgctcttcta ctccgggcag
      541 atcgagccgg cgatcgcgca ggtcgagcag gtgctcgcgc agacgccgac gttccagacc
      601 ggctggttca acaagggcaa ctacctcgcg gagaaggccc gtatcgcgga acaggacggg
      661 agcgccaagg tggccaaggc ggcgtacgcc gacgcgcgcg cggcctacga gaaggccgtc
      721 gcgctcggcg ccgacagcgc ctccggccag caggcccagc agcggatgga cgcactgccg
      781 aactaggacg cgcggcgagg ccgggtgtag agtcatcctg atggtcgagc tgctcccaga
      841 gatcacgcaa gcgcgcaagc tgacgccgag ggagcgcgcc gccctcggta aagcggcgcg
      901 caagcgcgcg ccacgctcgg cgcacgcgcg ctgggaggtg tccgccggcc ggccggaccc
      961 ggtcggtctg ctcgaggaac aggcggccga ccgggtgtcg gacctggtgc ccatccgcta
     1021 cggccgtatg ctcgtctcgc cgggcacgtt ctaccgggga ggcgcgctgc tgatggcgac
     1081 ggatctcgcc ggcacccccg actccgggct gcgggcgcag ctgtgcggcg atgcgcacct
     1141 gctcaacttc ggcctgttcc agtcgccgga gcggcgtctc gtcttcgaca tcaacgactt
     1201 cgacgagacg ctgccgggac cgtgggagtg ggacgtcaag cgcctcgccg cgagcttcga
     1261 gatcgcggcg cgcgaccgcg gctttcccgc cgccacgcgc cggtctatcg tcctgcacag
     1321 cgtccgtgcc tatcgcgagg ccatgcacga gatggcggag aagcgcgcca tccagctctg
     1381 gtacacgcgc ctcgacgccg acctcatcag ggagcacgtg acggtggtgg gagccaggga
     1441 cgccaggcgg atggcacggg acatgtcgcg cgcggagacg aaggacgacc tccgcgcgct
     1501 ctcgcggctc acctgcgagg tcgagggcag gacgcgcctg cgcagcgccc cgccgctgct
     1561 cgtgccggtc cgcgagctgc tggagggaga cgagcgcgcg cgcttcacgc actccgtcga
     1621 gcaggctctt cgcgcctatc gggagaccct gcccagagac cgccgggcgg cgtacgacct
     1681 ctatcgctac cgggacatgg cgcgcaaggt cgtcggggtc gggagcgtcg gcacgcgggc
     1741 gtgggtgctg ttgttcttcg gccgggacga aggcgatccg ctgttcctgc aggccaagca
     1801 ggcgcaggcc tcggtgctgg agcgcttcgt cgggcgcagc cggttccgcg gcgcgggccg
     1861 gcgcgtggtg gagggtcagc gcctcatgca ggcggtcggc gacatcctgc tcggctggta
     1921 cagcgtgacc gggttcgacg gccggcccta cgacttctac gtgcgccagc tgtgggacgg
     1981 caagggcacg ttcaacgtca ccgcgatgac ggagggcgcg tggcacacct acgcggagat
     2041 gtgctcgtgg gtgctcgcgc gcgcccactc gcgctcgggc gaccgcatcg ccatcgccgc
     2101 gtacctcgga ggcgcggacg tcttcgaccg tgccgtggcc gatttcgccg atgcctacgc
     2161 cgagcagaac cagcgcgact acgacgctct tcgcgcagct gtggactccg ggcgggtcag
     2221 cgcgcagggc ggcgtctgag gggccggccc gcgcgcggcg gcgccgggca ggcgaagggg
     2281 agcgccacga tggaggccga ggggtacgag ctcgcgagtc tgatcgatct ggaccgcgtc
     2341 cagcgcctct gcgagagcct ctcccaggcg ttcgacttcg ccctcgccgt cctcgaccgc
     2401 tccggcaacg tcctcatcgc caccgggtgg caggacatct gcacccgctt ccaccgcgag
     2461 aacgcggaca cgctgagcgg gtgtctcgag agcgacctgc gcatcaaccg gcggctggct
     2521 caggggctgg acccgtccga gcactacgcc taccgctgcc gcaacgggct gtgggacgtg
     2581 gcctttccgc tggtcgtcgc cggtgagcac gtggccaacg tctacaccgg gcagttcctc
     2641 ttcgacgacg acgacgtgga ccgggccgag ttcgcgcggc gggcccggcg gttgggattc
     2701 gacgagcagg cctacctggc ggctctggac cgcgtgccgg tcatctcaca cgagcgactc
     2761 cagaagaccg tccgcttcct cgccgacttc gtcggcatgc tcggcgagat gggggtcgcc
     2821 gcgctgcagg agcggcgcaa gcacgcgcag ctggtggaga gcgaggagcg ctaccaccgg
     2881 ctcttcgaca acgcgaccga ggggctcacc gtgttccgcg tggaacgggg accgggcggt
     2941 gaggtcgagg acgtcgtggt cgtcgacctc aaccccatcc aagccgagcg gacgcgggtc
     3001 acgcgcgctc agctcatcgg tcgcaggctc agcgagtgcg acggccacga tgagcgctta
     3061 cgcgcctact acgacgtggt gaggggcgcc gcggaggctc ggtgcgccac gcgcagcgag
     3121 gcgtgcctgc acgcgcggga cgcgtacgag ctcctgtccg cctacccggc gggagacgag
     3181 gacctgtggg tgctctcggc cacggacgtg accgacctcc gcgaggcgga gcgggcgctg
     3241 caccgccagg acgaggccat ccgcgcagcg tacgtcgacg tcctggacgc ggtgaccggg
     3301 ggcaagctca tcctcctcac ggaggagcag ctccccgagg agctggggag atcgctcggc
     3361 cggcagttcg ccttcggcgc gccgtcggag cttgcggcgg ctcgcggcat gatcgtgcgc
     3421 gcggcggagg cctgcttccc cggccggatc cgccgcaacg atctgctgag caccgcgggc
     3481 gaggcgctgg acaacgcggt caagcacgcg ggaggcggca cgttccaggc tttctgccgc
     3541 ggcgagagcc tgcaggtcgc gatcaccgac gaaggcccgg ggatcgactt caggaccctg
     3601 ccccgggcca cgctcgtgcc cggcttctcc accgccgcga gcctcggcat gggcttcacc
     3661 atcatgctgc agctctgcga gcgggtgctg ctgtccacgc gacccgggcg caccgtggtg
     3721 gtgctcgaga tcgccgccgc cggggagccg gcgcgggatc agtcggcgcg cgggcctgcg
     3781 ccggcggacg gatccgggct cacctcgcgc agcgcggtca ccagcgagtc cggcgtgtcg
     3841 ccgaacgtcg cgtaggccgc cgcgccgcac gcgtgcgcgc gcgagcggtc ctgcgcggtg
     3901 gtgaggacga tgaccggcgt agggtcgtcg cggtccatga ggtccgcgac catccggctc
     3961 gacaccgcgt caccgaggca ccagtcgacg aggaccacgt ccggtcgcga ggccgcggcc
     4021 cggtccatca ggctgcccac gtcgtcgacg acatcgacga cctggatgct gcgctcggag
     4081 aggaagaggg tcagagcgtg cccgaggtcc ggccgatcgg tcgcgagcag cacacgcatg
     4141 cccccctcct tccgctcggg atgctcgttc gagcgcctcc tcgcgcatca tcgcgcgccg
     4201 ggacggcgcg cgcctcgggc atacggccta tccgccccct gacaagtgtg ataggcgcgc
     4261 cgactgcggg gctcaggtga gcccgtgctt catcgcgatc gcggcggcgg tggtgcggtt
     4321 gggggcgccc agcttggaga ggatgttgct gacgtgcagg cggacggtgc ccacgctcag
     4381 gatgagccgc tccgcgatct ccttgttgct gagcccgccc gccagcagcg cgagcacctc
     4441 gcgctcgcgc gcggtgagat tcccgcccac gttgtcgtgg cggcgctcca tgagcgcgcg
     4501 catgacaccg ctgtcgatcg tgccgcgtcc ctcgacggcg tccctgatgg cctgggtgag
     4561 cgcctcgggg cgcgcgtcct tgagcaggta gctgatcgcc ccggcgtcga gcgtcttctc
     4621 gacggactcc tggtcgatgt agctcgtgag ggcgatgacg cggacgcccg gacagttggc
     4681 catgatctcg gccgtggcct cggcgccgtc catcaccggc atgcggacgt ccatgaggac
     4741 cacgtgcgga tcgaggagcc ggcactgctc ctgggcctcg agcccgttcg ccgcctcccc
     4801 gaccacgtcg atgtcggcgt acgtggacag cagcaccttg aggccctcgc gcaccatctg
     4861 gtggtcgtcg acgatgagca cgcggatgtc gtcgtggtcg ctcacgcacc cattctttcc
     4921 gctgctccct cgtcgtaagc agtcgatggc cacgcggcgc ggatggtcgt ccccccgccc
     4981 ggcgtggtgt ccacctcgag tgtgccggcg atgcgccgca ggcgctcgcg catgatgctg
     5041 aggcccatgc cgccggcgat ctcgtgagga tcgaagccgc ggccgtcgtc cgcgaccgtg
     5101 agtgaggcgc cgccgtcgac ggccgacacg cgcgcggtga cgctggaggc gcgcgcgtgt
     5161 ttggcgacgt tgttgaacgc ctcctgggcg acacggtaga agacgatctt gacgtccggc
     5221 ggcaggtcga tggtctcgtc ggcctcgatg tccaccgcca cctgcgtctg gccgccgagg
     5281 gcgtcgccga ggcgctcgag cagcgcggcg agcggcgccg actcgagggc ggcgggacgg
     5341 agctcgaaga gcagcgtgcg catctcggcc atggcggcgc ggacgagccg gcgcaggcgc
     5401 tccaggttgt gcgcgccctc ggccgggtcc cgctcccaca cggccggcag cgcctcggcg
     5461 atcagcgtgg cgctgtagat gctctgcgtc accgcgtcgt gcaggtcgcg cgccagcgcc
     5521 tggcgctcct cctcggccgc ctcctcggcc gcccggtggt agagccggtc gatctcgatc
     5581 accgcggcga gcgcctcgcc gatcgtgcgc gccagctggc gctcctcctc cgtgaagggg
     5641 ctggcgccgg cgtcccgggc gatgaccagc gcccccgccg ggcctgagga cgccaccagg
     5701 ggttcggcga ggaccggccc caccccgtcg gcccgggcct gccggcggac cgtctcgggc
     5761 aggccgggcc agtcggcgac gtcctcggcg accatctcgc agtcggccat gaccctgtcg
     5821 gtgaccgcca gatccaggtc gctgagcgcc tggatcccgt cgcggccggc cgcgcgcccg
     5881 tcgcccgccg ccggcggcgc ggccgcgtcg aggcaggcgg ccgcctccag cgcggcctcc
     5941 ccgtcggtca gaagccgcac gcggacgtag cgtgccttga agaggccgga ggtggcgctc
     6001 gtcgcctcgt ggagagcctg gccgagcgtg gtgcgccccg cgaggatctg cgccatgcgc
     6061 gtgaggacgt cgagttcctc gaccctgcgg cgcagcacct cttcggtggc gcgccgctcc
     6121 cttgactcct cggccagacg ctcgtgggcc tcgtcgagct cgcggacgcg ggcctcgacg
     6181 agctcgtcca tgtgcgtccg gtacagctgc agctccatct ccacccggag cgactcccgc
     6241 cgcagcacga ggctcccggc gatgatgacc gccgcgaagc tgacctgcga gaggtacagc
     6301 gtgttgaagg tgatgaccac ccggtgctcg atgaccgcgt cgaacagcgt ggccacggcg
     6361 aggagcgcgg tcatgagccc caggtagcgg gcgcgcgtct gcgcgggccg gcgggacacg
     6421 cgatacaccg cgtagcacag gaacccgaag gagatgagcg tcagcacgtc ggtcacgttc
     6481 tggaggatgt gcggcgacga ttccgtcatg accatgaccg agccgcccat cgcgtccatc
     6541 tccatgagcc cgcccctctg ctcgtggagg atcccgcgcg gcaggacgac gttgacgacc
     6601 agcgtgaaga cgaagccggc cgtcagcgcg agcaggaagc gcatcggccg cacgtcggcg
     6661 acgaaggcga cgaaccagac gaccgccgcc gtccacacga cggtcgccgg gaagtacgcc
     6721 cacttcatga cggcgatgtg caggtcgatg tcgtggatcg tgtacagggc gagcacggag
     6781 agtgcgccgg cggccgccgc cgccgccgcg atcgcgaagg cgatgcccgc gcggtcgggc
     6841 ggccgcgtgc tgaagccgac gaggccgtgc gtcagggccg ccaggatggc gatccccgcc
     6901 gccacgccca tgagcacctc tggtaggttc acccgtccct ccgcccggtc agggcacgct
     6961 cacctcggcc gccgccgacc acgccgacca gccggcggcg gactccgcgc gcacgcggta
     7021 gaagtacgtg ctcccgctct gcgcggtcgc gtccgtgtac gtcgtcgcgt cttcggcggc
     7081 ggtgaacgcc tgcacgtccg agctgaagcc ttcgtcggcc gctcgctcga cgacgtagcc
     7141 ggtcacgggc ggcgggaaca gcggcgccgt ccacgcgagc tcgacggcgg cgccggccgc
     7201 cgccgccgtc acgcccgacg gcgccgtggt cgtcccttgc aggaccagcg gacggacggt
     7261 gtgatgctcc tcgccgccct gcagatggat accccagtac gcctcgaaac cgagctccgc
     7321 cggcgcgttg acgaccggac gcgccagcgg cgccgcggtg acggggtcca gctgcgtgaa
     7381 cgcgccctcg gcgcccagcg gcagcgtgac gtccagcgcg cgcgagctgg cgggcagctt
     7441 gaacggcagc tcgggcagca cgggccgcat cgcgatgagc accgactcca gcgggtcgac
     7501 gcgcagggtg tccttccagc cgagctcggc cgggtcgggc gcgcgcgtct cgccctcgcg
     7561 ccgcgcgcgc gccagcacct gcacggcgaa gccggtgaac gtcacgctgt gcgagaggct
     7621 gccgtcgtgc gtgacgcgcc agatctgcgt ggcgtcgccg atctgcggcg cggtcgcctc
     7681 cccatccagc gcgagatact cggtggcggg gtcgatcgcc gagtagggga cggccgtcct
     7741 gatgccgatc gccgagagcg gggcgtccac cccgagcgtt cccgtcttgc gaccgaagag
     7801 cgggtcgaag aggtcgctca ccatcttccc ctggacgggg agggtgagcc ggtagacccg
     7861 ctccaggggc gtgaacgtca gggtgccgga ctcctcctcc gggacgtccg gcgccgccgg
     7921 atcctgcccg tcggcgtcgg gctcgagagc gtcgccgtag acatcccggt acgcggccgt
     7981 cggcaccagg acgggatcct gcgccgccgc gtaagcggcc ggaagcgccc gctggagccg
     8041 cgccacgtcg aacggcggcg cggccggtcc gtccacctgg aactgcatga ccgtgcgggt
     8101 gctggggccg tacccggact cggtggccgg agcgccgccg atcgccgtct tgtcgcgatc
     8161 gcccgtgtag tagtcgacgc ggccgtctcc gttgggggct ggggccggcg cgtcgttgta
     8221 caggatcagc ttggagccgg ccgggacctt cgagaagtcc acgatgacgt cggcgcgctc
     8281 gccgggcgcc aggtagagcg ccttggtcgt cacggcgacg atggacgcgt actcgcccat
     8341 cgacgcctcc tggtcggtgg ggagccggtt ctcgaggccc acgggcgtgg gcttgtgcgt
     8401 caccgcccgg ggcagaaggc cgccgtcgtt ggcgatctgg atcatcgccg gtccggcggc
     8461 cttggggtcc ggcacccctc ccacgcgggt gtccgtcggc cagcgcgccg gccagccacc
     8521 gctcatcgac ggctgcgccg gcagcatgga cacgtcgccc gaggctgtct gcagctcggg
     8581 ggagccgtcc gcggcggtct cgtcgatctc gttggacgtc gcgtagtaga gctgcaggtt
     8641 cagctggcgg tcgccgcagg cgttgaggat gcgcagccgg taggccttgc gctcgacctc
     8701 cagatacggg taggcggtcc cgttgaccag catggtgtcg tggaacgcgt tgggcaccac
     8761 ggaggggctg ggcgtgccgg ggttctcctc gggctgcgag gggaccgttc cgaagagcgg
     8821 gttggcgacg gggccgttct ccgtccccgt gtagccggtc cagtaccagg ggaggtagtc
     8881 ccagcgcccc ttggcgttga tcgtgggctg gtcgctgacg gcgccctcct cgcccacgcc
     8941 gttctggttg ggcatgtaga cgtgcggata ccagagggcg ccgaggcctc cccagcgggc
     9001 gatgtcccag gtgggatcgg tggtccgcag ccgttccgcc gccgggacga aggtcttgtc
     9061 ctcgatgacg agcggcagct cctcggccgg gacggtcccg gtctccacca gctcgtcctc
     9121 tacctcgtcg cccaggaggt agggcgccgc ctcgcccgcg tagacgctga ggcgcgtgag
     9181 gccgtaggtg tcgtcgtgca ggcagaggaa gcgggcgctc tggtcgttgg ggaagtagag
     9241 cgtggtggcg cccgggcccg ggtcgttggt ggctccgggc gcgccctcgg cgaccggcac
     9301 gccacgggcg tcgaaccaca tgtcggggac gttgaccagg ctgggcccgg aacggtaggg
     9361 gctcttctcg cccgccggcg tgatccactg gtactgcgag ccgccgctga tccagggggt
     9421 gagcccgccg tgcaggtgca gcgacgcgcg gttctgcggg tagacgtcct ctccgccgtc
     9481 cgggccggtc ccggtccccc tcgtcgtggt gtcgacgggc aggaagaggt cgccggccgc
     9541 cccgggcgcc agctggttga cgaacttgat gcgcaccggg cgcccgcggc gggcgcggat
     9601 cagcggcccg aggtagtgga taccggccgg ccgcacggtg ttgcgcccgc tcgcgtccgt
     9661 cccctggttg agctgcacgt agccgcggag gcgggtccgg gggaggtcgc tgtgcagctg
     9721 ctccgagtac tcgcggaccg ccagctcgta gtagtccgcg ccgggatacg tgaccgtgtc
     9781 gggcacggcc accgggatgt actgcccgag cgcgttggcc gcgtcgggac cgacacccgg
     9841 gagcctgtcc acgaacttcc gcagccgcgg gctgttggcc cagttggcga tgccgaagta
     9901 gtcgggggtc gcgccggggt cgaggcgcgg ctcgggagct gcgggtccgg cggcatcctt
     9961 ctgggcggcc agcgacgcgg cggcgaagcg ggcgtcctgc gagggcgcgt cggccatgat
    10021 ggcgatgagg tcgcgggcga ggtaggtggt ggcgccgttg gtgacccccg cgtcgacggc
    10081 cgaggcccct cgcggcgcga cgccggcctg cggccccgcc gcctcgtcgc cgctgcaggc
    10141 ggcgagaccg gcggcgagcg tgaccgctgc ggtcagcgcg gccagagccg ccgtcagcct
    10201 tgccggtctg tggccgtcca ggtcgccctc ccgaggtccg ccccgtccgc gtcgccgcaa
    10261 gcgattctgg acgggctcaa aacgctacca tacaatcgcg gcagcatggg tcccgacgcc
    10321 ggacgggacc cgcttccggg aaggggcctc agtgaaagtg ctcgccgtcc tcatcatcat
    10381 catggggctg gtcatcatca tcgtgccgca gtacaccaac tgcgaggccg gcaaggacca
    10441 gcctgccacc ctcaacatgc agaccagcga cgccggggtg atcgaggtcg cgagcacggt
    10501 ggccatggag ggcaccatgg agtccgccgc cgtgccgtac cgcatgatga agtgcctctg
    10561 gtcggcgcgc gcggagatcg tcgccggcgt cccgctcatc gcgctcgggg cgctgctcct
    10621 cttcgcccgg cgcaaggaga ccatccgcgt gatcggcatc gtgaccacgc tcctcggggt
    10681 cctcaccatc ctcatcccga catccgtcat cggggtctgc atgaaccccg agatggtctg
    10741 caacacacag atgaagccca ccctgctcat cgccggcggc atcaccgtcg cgctcggcat
    10801 cgccgtgctg gtgctgggcg agatgcggcg ccccaccgac ggccccgcgg aggccgcggc
    10861 cgccgcgtga gagggtgccc ggcctgagcc ctcatagctt gatcaggctc gcccgctcgg
    10921 ggatctccag caaccccctg cggagctggc tcgtcggcgg ctgcgcgctg ctcgtcgccg
    10981 ccatggtgct caccacggtc gtcatctccc gcggcggcca ggagagcctg cgcctcgcgc
    11041 gcgagcggct cggcgcggat gtcgtggtgg tcccccgcgg atccgtcagc ggggtggagg
    11101 gcgcgctgct cctgggcaac gcgaccaaca cctacatgcc gcgcggggtg gtcgacaagg
    11161 tcaggagcgt gcccggcgtc gctcaggcct cgccgcagct ctacctcacg tcgatggcga
    11221 actcgtcctg ctgctcggtg tcgctgatgt tcatgttcgc cttcgacccg gtcaccgact
    11281 tcaccatcga gccctggctc caggagaagc tcggccagga cctgcaggag ggccaggccg
    11341 tcggcggctc ggacgtgttc gtcccggagg gcgaggacaa gatcaagctc tacggctacg
    11401 atttcgacct cgtcgccaac ctcgagcgca cgggcaccaa cctggaccag acgctgttca
    11461 tgacctaccc gacggcatac gccatgcagc aggtgtcgca gaccgaggcc aagatggcgc
    11521 tccccgtcct ctccgacacc gtctcgtcgg tcctcgtcaa ggtcgccgac ggcgccgatc
    11581 gcagcgaggt cgccatggcg atcggcgagg ccgtgcccga ggtggacgcg gtgccgtcgg
    11641 tgcagatgtt cagctcctac ggagaccaga tctcgggcgt gctgcgcacg ctcatcgtga
    11701 tcgtggtgct caccgtcatc ctgtcgctga cgctcatggc ggtcatcttc tggatgtccg
    11761 tgaacgagcg cagccgccag atcggggtgc tgcgcgcgct cggtgcgacg cagcgcttcg
    11821 tgctcctcgc ctacatgacc gaggccgccc tgctggcgct ggccggcggc ctcgcgggcg
    11881 tcgtgctggc cgcggtcgtg gtctacttct tccacgacct cctggtgtcc gcgctcgggt
    11941 tcacgatcct gctgccctcg gcgccgagcc tcatcgggct cgtggtcgtc ggcatgctgg
    12001 gcgcgctggc ggtcgcggcg gcggcggcgt tcgtgcccgc cctgcgcgcc tcgcgtcaag
    12061 agccggccgt cgcgatgagg gagtgaggga gatgaccgcg gtatccctgc ggggggtcgg
    12121 caaggcttac ggcgacaagg acgcggcgac gttcgccgcg ctgcgcgacg tcgacctcga
    12181 cgtcgagacc ggcgagttcc tcatcctcac cggacgctcc ggctgcggca agaccacgct
    12241 gctcaacatc gcctcgggcc tcgcgacgcc gacgagcggc agcgtcaagg tggagggcgt
    12301 cgacctgtgg agcatcagcg acgccgagcg cagcaacctg cgcaaccgca ccatggggtt
    12361 cgtcttccag ttcccgagcc tgctgcccgg gctcacgctg gcgcagaacg tgacgctgcc
    12421 gctcgagttc agcgccgagg agcacgccga cgccgccggg cgggcgcgcg agctgctcga
    12481 gacggtgggc ctcggccatc gcgccgacgc gctgccgcgc gagctctccg ccgggcagca
    12541 gcagcgggtg gtcgtcgccc ggtcgctcat caaccgcccg acgctcctgc tggccgacga
    12601 gccgagcagc gatctcgacc aggacaccga ggccgagatc atggagatct tcagccgcat
    12661 ccacagggag cagggcatca ccatcatcat ggcgacgcac gcgcgcaacc tcatctcctt
    12721 cggcacccgt cacctcgaga tggccgacgg gaccctgcgc cccgacggtg ttgccgcggc
    12781 aggcaacggg gattgacgcc cccagctggg cgcgtcgaga cgcggttccg agcgtcccgc
    12841 ccagcatgcc tatgacgttt gtctaggtga agtcaggcct tccagccgtg ttcgctacct
    12901 gagaaatcgg gtcatgatta gcccggattc gtcccggtgg ggcgcgagcg cctgcgtggc
    12961 cccaccggcg gactcaccaa catgcgtcgg caacgacgta gaaaagtcgg ggttgtaggc
    13021 acaggggaag gggtttcaga tggctgccag agtcgcacgg cacatgtcgc tggcgagggc
    13081 cagcatcctc gtgttcatcg tggtggcgtt gctcaccgcg ctgttcatgg tcaagatgag
    13141 ccagggcgaa gcgatgccca caggcccgac cgacagcacc accgtacccc actactttgg
    13201 accctggccc aactgggccc tgagcccgct caccctgccc gacgccaccg tcaccatcga
    13261 cggtgacggt acgggcgccg cggccgtcgc cacggtgggc ggcaacggcg tcatcacagg
    13321 gatccacgtc accaacccgg gcagcggcta caccacggcg acggtcggca tcacgggagc
    13381 cgggaccggg gcagcggcga gcgcgacgat caccagcaca ggggcgatcg tggccgtcac
    13441 cgtcggcaac cccggcagcg gctacaaggc gcccgtcgtc accatctccg gcggcggcgc
    13501 gaccgcggac gcgaccgcga cggcctacgg cggcgtcgac gcgatcacgc tgcagaacgc
    13561 cggcaccggc taccagttcc cgacggtcga cttcgacatg ccggacgacc ccaacggcat
    13621 caaggccgag gcggccgcga cgatcgacgc caacggggcg atcaccggca tcaccatcac
    13681 caaccccggc tcggggtacg ccacggcgcc gaccgtcgtc atccgcgacg gcacactgat
    13741 ggacccggtc gccaacgccg gcgcgggcgc ctcggcggtc gccaccatca acgtcaccac
    13801 cgtgcacctc ctcacgttcg gcgccggcta tacctcggct ccgaccgtga ccatcacgga
    13861 ctccgtcggc accggctccg gcgccaccgc cacagctgcg gtgaacaacg gcctcatcag
    13921 cgcgatcgac gtgacggccg ccggctccgg ctacgtcacc ggcggcggca tccggaagtt
    13981 ccaggaccag ctgcccatgc tctgcaaccc cgccgtcccc ggcagttgcg aggatgccgc
    14041 ggccgcgaac aacctcggca agttcctccc cctcggtgtg cccgaggaga agacctaccc
    14101 cgacccggac ggcaacccca tcctgtccga cgagtacgag atcggcctta tccaataccg
    14161 gtccaagttc aacaccgacc tgccggcgac gctcgcgcgc gcctacgtcc agatcgacac
    14221 gtcggcggac gctgcgacca ccggctaccc gctggagaac gagctcctcg acgggaccaa
    14281 ggtgccggtc atgatcgacg gcctgcaggc ctacggcgtc acctccccgc agtggctcgg
    14341 cccggtcatc gccgccacca agaacaagcc ggtgcgcatc gtgttccgca acctcctgcc
    14401 gaccggctcc gacggcgacc tcttcctgcc caccgacagc acgctgatgg gctccggcat
    14461 gggccccatg ggtctggccg acccggtcga cgagggctcc gtcatggacg gggtgcgcaa
    14521 ccccgtctgc agcgaggcgc ccaagtcgcc cgactgcttc aaggacaacc gcgccaccct
    14581 gcacctccac ggcggcatca gcccgtggat cagcgacggt acgccgcacc agtggatcac
    14641 ccccgccaac gaggacacgc cgtggcccga gggcgttgac gtgcgctacg tgcccgacat
    14701 gcccgtgggc aacgacccgc gggacggcgt catgaccttc tactacacca accagcagag
    14761 cgcgcggctg atgttctacc acgaccacgc ctggggcacg acgcgcctca acgtgtacgc
    14821 gggcgaggcc gccggctaca tcatctccga cga
//
LOCUS       DASUMX010000195         8584 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1218270, whole genome shotgun sequence.
ACCESSION   DASUMX010000195 DASUMX010000000
VERSION     DASUMX010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 8584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8584
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1218270"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..801
                     /locus_tag="VFZ86_09725"
     CDS             82..801
                     /locus_tag="VFZ86_09725"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX5642600.1"
                     /translation="MRIAAIADLHCGVEGGPEMSRILQGVRDAADVLLVGGDLTNLGL
                     LEEGEELLRALEGLRLPVVAVLGNHDHESGRAAELAALLRDGGIHLLDGTAWELDGVG
                     FAGVKGFAGGFTPHRLAAFGEAAIKAFVEVAVREAAALDHALASLETRHRVAVLHYAP
                     VAATLAGEPPQIHPFLGDSLLEEVLDRHGAAVAVHGHAHDGFAEGLTSGGIRVLNVSR
                     SVLGRRPGGQSYVTFDVPARG"
     gene            complement(814..1884)
                     /locus_tag="VFZ86_09730"
     CDS             complement(814..1884)
                     /locus_tag="VFZ86_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019607752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HEX5642601.1"
                     /translation="MRTPRLRRSDTGAPGIRRRRRGGSFFYLWPDGERVRDEATLQRI
                     ADLKIPPAWRQVWISPWPDGHLQALGTDAAGRRQYLYHERWRLRRESRKFARMEAFAA
                     ALPGLRRQVSEALALPGLPRERVLACAVRLLDRAYVRSGGSEYVANGSFGLATLRREH
                     AVVRAHGRILLKFPGKAGVLQRRSLVDPEAGRVLRSLKRRDGGGEELLAYRGRDGAWV
                     DVRSADINAYIKQVTGGDFTAKDFRTWHATVLAAVALAVSVPAMKPESARRRAVARAV
                     AEVSDYLGNTPAVCRRSYVDPRVIDRHREGRTIVAALDRVGGCAAQDDTEAWEGIEAA
                     VLGLLDGAVPRLDRRFAWLVAA"
     gene            2079..2531
                     /locus_tag="VFZ86_09735"
     CDS             2079..2531
                     /locus_tag="VFZ86_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX5642602.1"
                     /translation="MATVESSIDVNVDLTTAYNQWTQFESFPQFMENVKSVHQLDDRR
                     LRWVAEIGGQTREWDAEIVEQIPDQRIAWSSVGGEPNGGIVTFQSLGDTTTKVHLRME
                     YEPQGLLESAGDLLGVVDRQVKGDLERFKRFIESRGEETGSWRGTVAV"
     gene            2656..3720
                     /locus_tag="VFZ86_09740"
     CDS             2656..3720
                     /locus_tag="VFZ86_09740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX5642603.1"
                     /translation="MIDRTQVKLEEELRQSREDLDRAQAVGHLGSWRLDIRENVLTWS
                     DENYRIFGMPVGAPLTYETFLGVVHPDDRALVDRRWRAGLRGEPYDIEHRLLVAGRVK
                     WVREKAFLEYDESGRLLGGFGITQDVTDRRAAEEALRESEAERAKQLERARLARDLHD
                     SVSQAIFAASLKAEALSEAGKCADPQIAETAAQVSRLCRGALAELRTMLLELRGERLE
                     DLPMETLLRQLVDAIEGRTSTQVGLTVEGTSALPTAVHVAFYRVAQEALNNLARHARA
                     TRARVEASLGEAEARIEVSDNGCGFEPRDFGPGHLGLRSMRERAAEIGADLTLASARD
                     EGTRVILLWRDDQSAGHRPT"
     gene            3919..5331
                     /locus_tag="VFZ86_09745"
     CDS             3919..5331
                     /locus_tag="VFZ86_09745"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5642604.1"
                     /translation="METSPQRHSLWVATTEMPTYSPLPGDTRAQVLVIGGGMTGLQTA
                     YLLKRAGAEVVLIERHLLGRGVSGHTTAKITALHGLAYHRLEGMFGPDKASVYADANQ
                     RALEAMLELIATEAIDCDLMRQAACTYAVTPQEREEVELEAESARAAGLAADVRDDLP
                     LPYETRGGVWLADQAHFHPGHYMKALAELIDGGGCRLHEQTTALSLDDEAMSVTTDRG
                     VVTAEHVVVATHFPVFDHGPLSMRAMARRSYVVAGRASEPPQGMFITVADDHKSLRPQ
                     TTADGASYLLMGGEPHRTGEGGDTRARYQALEGWAADTFQPDALEFAWSTQDIWTNDD
                     VPFIGRFRKDIDRVWMATGFKGWGMTHSMVAARIMSEAIQGRSTPWSELYDPWERSLL
                     HGAGKLLSQAAVSIKSLVFEKMVSGDPACTHMGCTTQWNEAEGSWDCPCHGSRFTESG
                     EVLHGPAIEPLKTKPTPTTE"
     gene            5389..5520
                     /locus_tag="VFZ86_09750"
     CDS             5389..5520
                     /locus_tag="VFZ86_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642605.1"
                     /translation="MPAVPTKDEYMQRCICGDCETFKEFGMSKGYCCAEGYAEEIEA"
     gene            6315..6737
                     /locus_tag="VFZ86_09755"
     CDS             6315..6737
                     /locus_tag="VFZ86_09755"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX5642606.1"
                     /translation="MDIFEELHSEHEEVSKLIAKLESGRDEATFETLRTELTAHSKAE
                     EQVFYKRLEDEESVRDTVLEGYEEHKVVVKVLTDMAQTTSDDEKFMAKLAVLKESVEH
                     HVEEEEGTLFEGARPLLAEGEPESLYEQFEAAKSKLEG"
     gene            6753..>8584
                     /locus_tag="VFZ86_09760"
     CDS             6753..>8584
                     /locus_tag="VFZ86_09760"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017154698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HEX5642607.1"
                     /translation="MAEKKARQAKRKPAKRAKRAAPPEVARGAADEVHQRAEGDAPAL
                     TTNQGLRISDNQNSLRAHRTGPTLLEDFVLREKIFHFDHERIPERIVHARGSAAHGFF
                     ELTDSLAEYTTAKVLTEVGEKTPVFARFSTVAGGAGSIDTPRDVRGFAVKLYTKEGNW
                     DLVGNNIPVFFIQDAIKFPDIVHAVKMEPDRAFPQAASAHDTFWDFISLTPESMHMVM
                     WIMSDRTIPRSLRMMEGFGVHSFRLVNAAGESTFVKFHWRPKLGLQSTLWDEAVKIAG
                     ADPDFHRRDLYEAIESGAFPEWELAVQLFTQEEADKLPFDHLDATKLIPEEIMPLRVI
                     GRMVLDRWPDNFFAETEQVAYCPANIVPGIDFSNDPLLQGRLFSYLDTQLSRLGSPNF
                     HQLPINAPKCPFGNHQRDGHMQMTQPKGRVAYEPNSLADTPDDLPREAADGGFTSFAA
                     AESGEKGRIRAESFADHYSQARQFYLSQSPPEQAHLASALVFELSKVAHVHIRQAMVG
                     HLRHIDADLAKRVAAGLALDPLPEAPPTAVAPVDLDRSPALQLIGKMKDTLEGRQVAI
                     LVADGSDGDAVQALKAAATEAGATVKIVAPKVGGATLADGTLLEA"
BASE COUNT         1429 a         2983 c         2974 g         1198 t
ORIGIN      
        1 gttccgggtc gacgtcgccg agcgaggcta tcgcgacgcc ggtgctcatc cccccatcct
       61 cgatcgctcg gggagaccgc tgtgaggatc gccgcgatcg ccgatctgca ttgcggcgta
      121 gagggaggcc cggagatgag ccgcatcctt cagggcgtgc gggatgcggc ggacgttctg
      181 ctcgtgggcg gcgatctcac caacctcggc ctgctcgagg agggcgagga gttgctgcgc
      241 gcgctcgagg ggctccgcct ccccgtggtg gcggtgctcg ggaaccacga ccatgagagc
      301 ggccgcgccg ccgagctcgc cgcgctgctg cgcgacggcg gcatccacct gctcgacggc
      361 accgcctggg agctggacgg cgtgggcttc gcgggcgtca aaggcttcgc cggcgggttc
      421 acgcctcatc gcctcgcggc gttcggcgag gcggccatca aggccttcgt ggaggtggcc
      481 gtgcgcgagg cggcggctct ggaccacgcg ctggcgagct tggagacccg gcaccgcgtg
      541 gccgtcctgc actacgcgcc ggtcgcggcg acgctcgccg gcgagccgcc gcagatccac
      601 ccctttctgg gcgactcgct gctcgaggag gtgctcgacc ggcacggcgc cgccgtggcg
      661 gtgcacgggc atgcccacga cggcttcgcg gagggcctga ccagcggcgg catccgcgtg
      721 ctcaacgtct cgcgcagcgt cctcggccgg cgcccgggcg gtcagtccta cgtcacgttc
      781 gacgtgccgg ctcgcggcta gcgggaccgc acgtcaggcg gcgaccagcc aggcgaagcg
      841 ccggtccagc ctgggaaccg cgccgtcgag gaggccgagc accgcggcct cgatcccctc
      901 ccaggcctcg gtgtcgtcct gcgccgcgca accacccacg cggtcgagcg cggcgacgat
      961 cgtcctcccc tcccggtgcc ggtcgatcac ccgggggtcg acgtaggagc ggcggcagac
     1021 cgccggcgtg ttgccgaggt agtcggatac ctcggcgacg gcgcgcgcga cggcgcgccg
     1081 gcgcgcgctc tccggcttca tcgccgggac cgagacggcc agcgccacgg cggcgagcac
     1141 cgtcgcgtgc cacgtgcgga agtccttggc ggtgaagtcg ccgcccgtca cctgcttgat
     1201 gtacgcattg atgtcggccg aacgcacgtc gacccaggcg ccgtcgcgac cacggtacgc
     1261 gagcagttcc tccccaccgc cgtcgcgccg cttcagcgac cggaggacgc ggccggcctc
     1321 cgggtccacc aggctgcggc gctgcagcac cccggccttc ccggggaact tcagcaggat
     1381 ccgcccgtgc gcccgcacga ctgcgtgttc gcggcgcagc gtcgccagac cgaacgagcc
     1441 gttcgcgacg tactcgctgc ccccggagcg gacgtaggcg cggtcgagca ggcgcacggc
     1501 acacgccagc acgcgctcgc gcggcaggcc cggaagcgcg agcgcctcgg acacctggcg
     1561 gcgaagaccc ggcagtgccg ccgcgaacgc ctccatgcgt gcgaacttcc tggactcgcg
     1621 ccgcaggcgc cagcgctcgt gatagaggta ctggcgccgc cctgcggcgt ccgtgcccag
     1681 cgcctgcagg tgtccgtccg gccacggcga gatccagacc tggcgccagg cgggggggat
     1741 cttgaggtcg gcgatccgct gcagcgtggc ttcgtccctg acccgctccc cgtcaggcca
     1801 gagatagaag aacgaccccc ctcggcggcg ccggcggatg ccgggcgccc ccgtgtcgga
     1861 ccgccgcagc cgcggggtcc gcaccctcgt cgaggccgaa gccgttgcag acgaccctcc
     1921 catacttcgc ggcattcccc cgcttcctcg cgccgagacc ccgcaccgat gacgaggcgt
     1981 gcaacgggat cgccaggcca cggcatggcg tgtagaccgc cgagccggtg gaagacccaa
     2041 agagtaagga aggacaagcg acagggaggc cagcttcaat ggcgaccgtg gaatcatcca
     2101 tcgacgtgaa cgtagacctc accaccgcct acaaccagtg gacgcagttc gagagcttcc
     2161 cccagttcat ggagaacgtg aagtcggtcc accagctgga cgaccggcgc ctgcgctggg
     2221 tcgccgagat cggcggacag accagggagt gggacgccga gatcgtggag cagatccccg
     2281 accagcgcat cgcgtggtcg agcgtcggcg gtgagccgaa cggcgggatc gtgacgttcc
     2341 agagcctcgg cgacaccacg accaaggtac acctgcgcat ggagtacgag ccgcagggct
     2401 tgctcgagag cgccggcgac ctgctcggcg tggtcgaccg ccaggtcaag ggcgatctcg
     2461 agcgcttcaa gcgcttcatc gagagccggg gcgaagagac cggctcctgg cgcggcaccg
     2521 tcgccgtctg acgcggaggt cgccggcgcc gcgggagtac gccggggcca ccagcctaca
     2581 tcagctcgga ggggccggcg ggatacgccg gcccctccct cgctgcggcc gtgtgcggag
     2641 acgtatgcta tgacgatgat cgaccggacg caggtgaagc tcgaagagga gcttcgtcag
     2701 agtcgagagg acctggaccg ggcccaggct gtggggcatc tcgggagctg gcgtctggac
     2761 atccgcgaga acgtcctcac gtggtcggac gagaactacc gcatcttcgg gatgcccgtc
     2821 ggagccccgc tgacctatga gaccttcctg ggagtcgtgc atcccgacga ccgcgccctc
     2881 gtcgacaggc ggtggagggc gggcttgcgg ggcgagccgt acgacataga gcaccggctg
     2941 ctcgtggctg ggcgggtcaa gtgggtgcgt gagaaggcgt ttctggagta cgacgagtcc
     3001 ggccggctgc tcggcggctt cggcatcacc caggacgtca ccgatcgccg ggctgccgaa
     3061 gaagcgctgc gggagagcga ggcagagcgc gccaagcagc tggagcgggc gcgcctggcc
     3121 cgcgatctgc acgactccgt gagtcaggcg atcttcgccg catcgctgaa ggcggaggct
     3181 ctgagcgagg ccggcaagtg cgccgatcct cagatcgcag agacggccgc gcaggtgagc
     3241 cgactctgcc gcggtgcgct cgccgagctg cggaccatgc tgctcgagct gcgcggcgag
     3301 cggctggaag accttccgat ggagacgctc ctgcgccagt tggtcgacgc catcgaggga
     3361 cgcacgagca cacaggtcgg gctcacagtg gaagggacct cggcgctgcc gaccgccgtc
     3421 catgtggcct tctatcgtgt tgcccaggag gcgctgaaca acctcgcgcg acatgcgagg
     3481 gccactcggg cccgcgtcga ggcgtccctc ggtgaagcgg aggccaggat cgaggtcagc
     3541 gacaacgggt gcggcttcga gccgcgggac ttcggaccag gacacctcgg ccttcgctcg
     3601 atgcgggagc gcgcggcgga gataggagcc gacctgacac tggccagcgc cagggatgaa
     3661 ggcacccgtg tcatcctgct gtggcgggat gatcagtccg ccggtcaccg cccgacctga
     3721 gcgctacgcg ctgcgtattc gcccgcaccc tttcaggtgc cgccgcctct ccacgccggc
     3781 tccacccatg cgtcgacggc gtaagccttg cggccacggg tagtgcaaga ggagccttgg
     3841 ggagcgcctg agatcgggtt cctggctgcg accgtgggtc ggttcgctcc cctggccctg
     3901 cgcgacgaaa ggagccagat ggagacctca ccgcagcgtc actccctgtg ggtcgcgacc
     3961 accgagatgc cgacgtactc gcccctgcct ggtgacacgc gcgcgcaggt gctcgtcatc
     4021 ggcggtggca tgaccggcct gcagacggcc tacctgctca agcgagccgg cgcagaggtc
     4081 gtactgatcg agcgacacct tctcggccgc ggcgtgagcg gtcacaccac ggccaagatc
     4141 accgctctgc acgggctcgc ctaccaccgt ctcgagggga tgttcggccc cgacaaggcg
     4201 tccgtctacg cggacgccaa ccagcgcgcc ctggaggcga tgctggagtt gatcgcgacc
     4261 gaggccatcg actgcgacct catgcggcag gccgcgtgca cgtatgcggt caccccgcag
     4321 gagcgcgaag aggtcgagct tgaggcggag tcggcgcgag ccgccggcct cgcggcggac
     4381 gtccgcgacg acctccctct gccctatgag acgcgcggcg gggtatggct ggccgatcag
     4441 gctcacttcc atcccggcca ctacatgaag gcgctcgccg agctcatcga cggcggcggg
     4501 tgccgcctgc acgaacagac gacggcgttg tcgctcgatg acgaggccat gagcgtcacc
     4561 acggaccgcg gcgtcgtcac ggcggagcac gtggtcgtag ccacccactt cccggtcttc
     4621 gaccatgggc cgctgagcat gcgggccatg gcgcggcgtt cctacgtcgt ggcgggccgc
     4681 gcgtccgagc cgccgcaggg catgttcatc acggtcgccg acgaccacaa gtcgctgcgg
     4741 ccgcagacca cagctgacgg cgcgtcgtac ctcctcatgg gcggtgagcc gcatcggacg
     4801 ggcgagggtg gcgacacgcg ggcacgctac caagccctcg agggctgggc ggccgatacc
     4861 ttccagcccg atgccctgga gttcgcctgg tcgacccagg acatctggac gaacgacgac
     4921 gtgcccttca tcggccgctt ccgcaaggac attgaccgcg tctggatggc tacaggcttc
     4981 aagggctggg gcatgacgca cagcatggtc gcggcgcgca tcatgagcga ggccatccag
     5041 ggccgatcga cgccctggtc cgagctctac gatccgtggg aacgttcgct cctgcacggc
     5101 gccggcaagc tcttgagcca ggcggccgtg agcatcaaga gcctggtgtt cgaaaagatg
     5161 gtgagcggcg acccggcttg cacccacatg ggctgcacga cgcagtggaa cgaagcggag
     5221 ggcagctggg actgcccctg tcacggctca cggttcaccg agagcggcga ggtcttgcac
     5281 ggacccgcca tcgagcctct gaagacgaag ccaacgccga caaccgagtg acacaagcag
     5341 tcggcgcggt cgcaggcagc ctgaaccagt cgttcaacga gagacgccat gccggcagtc
     5401 ccgaccaaag acgagtacat gcagcgttgc atctgcggcg actgcgagac cttcaaggag
     5461 ttcgggatga gcaagggcta ctgctgcgca gaaggctacg ctgaagagat cgaagcctag
     5521 gaacgagggc ggagctgcgt cgccgggcaa gagccggacg tctccacgca ggagctgggt
     5581 ccggagtagc tcgcggaggt cctgacctca cgccgctcca gctgcctggc ggaggccagc
     5641 gcctgtccca tctcccctct cagccgatgc taccgatgag ggcctgcgat cgacacctcg
     5701 tgcagcggca atcctcgcga tgccgccatc ccccccgggg ggcgctccag atgggaatct
     5761 gctgcggctc actgcggccg cgtcggtacc agaagttgat gccgtcatag aagtacagac
     5821 gcgcgcgctc gtggtcctcc gtccaggccc tgcgctccga ctgcaccacc tcgtcacggc
     5881 tcatcgtccc gcatcgccgg gtcattgctc atcctccagt cttgccagcc gtcagaggct
     5941 cttgaggtgc tcacggcgcg atccttccgc gccgtgaggg cccgcacgtc tctcgcgccg
     6001 taccggggag atgagcttag gacccagggc ttcggtgcgc atcggccatg cgcactgatg
     6061 gggggatagc cgcaagcacg gtctctcact cggtcatctg accgacccga gatagccgtc
     6121 ccgtgccatc tcacagcgga cagagacgac ggcagggagc ttcatcacgc ggggacaacg
     6181 aggtgcagtt ctcgcccaca agccggcggt atccggcgta acggcgatgc cgtcacgcat
     6241 cgcatgagcc gggtaggagt tttgcgggcc gcacgcacgc ggccgtacac caatccgacc
     6301 cgggaggaga cccgatggac atcttcgagg aactgcacag cgagcacgag gaggtcagca
     6361 aactcatcgc caagctcgag agcggccgcg atgaggcgac cttcgagacg ctgcgcaccg
     6421 agctcacggc ccactccaag gcagaggagc aggtcttcta caagcgactc gaggacgagg
     6481 agtcggtacg cgacaccgtg ctcgagggct acgaggagca caaggtggtc gtcaaggtcc
     6541 tcacagacat ggcgcagacg acctcggacg acgagaagtt catggccaag ctggcggtgc
     6601 tcaaggagag cgtggagcac cacgtcgagg aggaggaggg cacgctgttc gagggcgccc
     6661 ggccgctgct cgccgaaggc gagcccgagt ccctgtacga gcagttcgag gccgccaaga
     6721 gcaagctcga aggctgaaac ggggggatcg ccatggctga gaagaaggca cgccaggcca
     6781 agaggaagcc ggcgaagcgc gcgaagcgcg ccgcaccgcc ggaggtggcg cggggcgcgg
     6841 ccgacgaggt gcatcagcgc gcggagggtg acgcccccgc cctgaccacc aatcagggcc
     6901 tccgcatctc cgacaaccag aactcgctgc gcgcccaccg cacggggccg accctgctcg
     6961 aggacttcgt cctgcgcgag aagatcttcc acttcgacca cgagcgcatc ccggaacgca
     7021 tcgtgcacgc gcgcggcagc gccgcgcacg gcttcttcga gctgacggac tcgctcgcgg
     7081 agtacacgac cgccaaggtc ctcacggagg tcggcgagaa gacgcctgtg ttcgcgcgct
     7141 tctcgaccgt ggccggcggc gccggttcca tcgacacgcc gcgcgacgtg cgcgggttcg
     7201 cggtcaagct ctacaccaag gagggcaact gggacctcgt cggcaacaac atcccggtgt
     7261 tcttcatcca ggacgcgatc aagttccccg acatcgtcca cgcggtgaag atggagccgg
     7321 accgggcctt cccccaggcg gcgagcgcac acgacacctt ctgggacttc atctcgctca
     7381 ccccggagtc catgcacatg gtcatgtgga tcatgtcgga ccgcaccatc ccgcgctcgt
     7441 tgcgcatgat ggaggggttc ggcgtgcaca gcttccggct cgtcaacgcg gcgggtgagt
     7501 cgacgttcgt caagttccac tggcggccca agctcggcct gcagtccacg ctctgggacg
     7561 aggcggtcaa gatcgcgggc gccgacccgg acttccaccg ccgcgacctg tacgaggcca
     7621 tcgagagcgg cgcctttccg gagtgggagc tggccgtcca gctgttcacg caggaggagg
     7681 cggacaagct ccccttcgac cacctcgacg cgaccaaact gatccccgag gagatcatgc
     7741 cgctgcgcgt catcgggcgg atggtcctcg accgctggcc cgacaacttc ttcgccgaga
     7801 ccgagcaggt ggcgtactgc cccgccaaca tcgtccccgg catcgacttc tccaacgacc
     7861 cgctgctgca gggacggctg ttctcgtatc tcgacacgca gctctcgcgg ctgggctcgc
     7921 ccaacttcca ccagctcccg atcaacgcgc ccaagtgccc gttcggcaac caccagcgtg
     7981 acgggcacat gcagatgacg cagccgaagg gtcgcgtggc gtacgagccg aactcgctgg
     8041 cggacacccc cgacgacctg ccgcgcgagg cggcggacgg cgggttcact tcgttcgcgg
     8101 ccgccgagag cggcgagaag gggcgcatcc gcgccgagag cttcgccgac cactacagcc
     8161 aggcgcggca gttctacctg agccagtcgc ctccggagca ggcccacctc gcctcggcgc
     8221 tcgtcttcga gctctcaaag gtggcgcacg tgcacatccg ccaggcgatg gtcggccacc
     8281 tgcggcacat cgacgccgac ctggccaagc gggtggccgc cggcctggcg ctcgacccgt
     8341 tgcccgaggc gccgccgacg gctgtggccc ccgtggacct cgaccggtcg ccggcgctcc
     8401 agctgatcgg caagatgaag gacacgctcg agggccggca ggtcgccatc ctcgtggccg
     8461 acggctcgga cggggacgcc gtccaggcgc tgaaggcggc cgccacagag gccggcgcca
     8521 cggtcaagat cgtggcgccc aaggtcggtg gcgcgaccct ggccgacggc acgctcctcg
     8581 aggc
//
LOCUS       DASUMX010000196         3883 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1218354, whole genome shotgun sequence.
ACCESSION   DASUMX010000196 DASUMX010000000
VERSION     DASUMX010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3883
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1218354"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /gene="manB"
                     /locus_tag="VFZ86_09765"
     CDS             complement(<1..656)
                     /gene="manB"
                     /locus_tag="VFZ86_09765"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="converts mannose-6-phosphate to
                     mannose-1-phosphate; the resulting product is then
                     converted to GDP-mannose by ManC which is then used in the
                     synthesis of mannose-containing glycoconjugates that are
                     important for mediating entry into host cells; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEX5642608.1"
                     /translation="MTIPDGIFKAYDVRGLYPQELDEAAAERIGCALAQQLGASRLGV
                     GMDPRPSSTGLLEAFAAGAAAGGASTVDFGLVPTEMLYFGVASRGLDGGAMITASHNP
                     PQYNGMKLVGEGALPLSGDAGIPELKERSQALEELPARSAGAGLERVDLYPAFVEHLH
                     GLVDVAAFRPYKVVMDAANGVAGKLAPRVFAGTAIDAVEMYFEVDGTFPNHSPNPLLE
                     "
     gene            complement(653..1813)
                     /locus_tag="VFZ86_09770"
     CDS             complement(653..1813)
                     /locus_tag="VFZ86_09770"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HEX5642609.1"
                     /translation="MSAARAIPRQAVILVGGEGTRLRPVTSRVPKPVAPVVERPFVAY
                     ILDNLALHGVERAIFSTGFLAEAIEAVIGDGAGYGLEVAYAVEEEPLGTAGAIANCDG
                     LLDEGSFYVFNGDVLSDVDLSALAATHLDKGGMGTIFLTPVDDPRRYGLVELHEDGSV
                     ASFLEKPGEWEGTALINAGVYVLEPEVLEMIPRGRLFSIERGVFPKLAAAGSLYGYVD
                     HGYWRDIGTPDSYLQAHFDILERQVRTSVADELGEQYLHISPSAEVDPAARVVPPCYV
                     ADGARVGAGARVGPLAVLGAGAVVGEGATVVEAVVQAGVVIGAHAQVEGSILVQGSSV
                     GAGTQLSGAVLGEGCVVGAGNRLAGGICVYPDTRLPDNSIQFYEQLRGREGS"
     gene            complement(1821..3134)
                     /locus_tag="VFZ86_09775"
     CDS             complement(1821..3134)
                     /locus_tag="VFZ86_09775"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEX5642610.1"
                     /translation="MDAAPIGVVGTGYVGLVSAVCFAHLGHDVVCMDVDEGKIARLER
                     GEVPIYEPGLEKLVTDNAARLSFTTSTDVLLERCRILFIAVDTPPSPSGDADLSRVQH
                     AVREIAARGGPRLLVMKSTVPVGTGDKITADLEAMGASDITYASNPEFLREGAAIQDF
                     LEPDRIVIGSFREEDGAAVEDLYRGIDTHVVRTSVPTAEMVKYASNAFLATKISFINE
                     IANVCEQVGADVRTVAHGMGLDHRIGPHFLQAGIGYGGSCFPKDVQALKQLAGNTGYH
                     FQLLNAVIEVNALQKRRVVGKLKERLGTLRGATIALLGLTFKPNTDDLREAASIVLAG
                     RLIHEGAKVMAYDPMLDAGEHPLFPGVAICADPLAALEGADAAVLVTEWQEFARLDWS
                     AAAATMARPLLIDGRNFIDPLVAAEAGLEYEGIGLGDEVPAPVAR"
     gene            complement(3235..>3883)
                     /locus_tag="VFZ86_09780"
     CDS             complement(3235..>3883)
                     /locus_tag="VFZ86_09780"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5642611.1"
                     /translation="ERRAARRSRHEGVARATATAPEQAAAAAAPWTAAGDAEADDGRA
                     AVRDDGTAVRERGEDGASGTARTTPARQARRSGWAPRVVASAALLLLAASWLPPYLAL
                     RAENAALAASSDGDVATALDHARRAAAWDPLAVGPLITEATLLQQLGRNREALERLQA
                     ATKLQPQNFVVWYELGVLQHGTLGRDKAARAAFTRALALNPDDAASRYELELLAQ"
BASE COUNT          529 a         1398 c         1422 g          534 t
ORIGIN      
        1 tcctcgagga gcgggttggg cgagtggttg gggaaggtgc cgtcgacctc gaagtacatc
       61 tcgacggcgt cgatcgccgt gcccgcgaac acccgcggcg ccagcttgcc ggcgacgccg
      121 ttggcggcgt ccatgaccac cttgtacggg cggaacgcgg cgacgtcgac gagcccgtgc
      181 aggtgctcga cgaaggccgg gtacaggtcg acgcgctcga ggccggcgcc ggcgctgcgt
      241 gccggcagct cctcgagcgc ctggctgcgc tccttgagct ccgggatgcc ggcgtcgccg
      301 ctcagcggca gcgcgccctc gcccacgagc ttcatcccgt tgtactgcgg cgggttgtgc
      361 gaggccgtga tcatcgcgcc gccgtccagc ccgcgagagg cgacgccgaa gtagagcatc
      421 tcggtcggga cgaggccgaa gtcgacggtg gaggcgccgc ccgcggcggc gccggcggcg
      481 aaggcctcca gcagccccgt ggacgacggg cgcgggtcca tgcccacgcc gagccgcgac
      541 gcgcccagct gctgcgccag ggcgcacccg atgcgctccg ccgccgcctc gtcgagctcc
      601 tgcgggtaga ggccacggac gtcgtaggcc ttgaagatgc cgtccgggat cgtcatgagc
      661 cctccctgcc gcgcagctgt tcgtagaact ggatggagtt gtcgggaagc cttgtgtccg
      721 ggtacacgca gatgccaccg gcgagccggt tgccggcgcc gaccacgcag ccctcaccga
      781 ggacggcgcc gctgagctgc gtcccggcgc cgacgctgga gccctggacc aggatgctgc
      841 cctcgacctg cgcgtgggcg ccgatcacca cgcccgcctg gaccacggcc tcgaccaccg
      901 tggcgccctc gcccaccacg gcgcccgcgc cgaggacggc gagcggaccg acgcgcgccc
      961 cggcgcccac acgggccccg tcggcgacgt agcagggcgg caccacgcgc gccgccggat
     1021 cgacctcggc gctcggcgag atgtgcaggt actgctcccc gagctcgtcc gccacagagg
     1081 tgcggacctg ccgctcgagg atgtcgaagt gcgcctgcag gtagctgtcc ggcgtaccga
     1141 tgtcgcgcca gtacccgtgg tccacgtagc cgtagagcga gcctgccgcg gcgagcttgg
     1201 ggaacacgcc ccgttcgatc gagaacaacc gccctcgcgg gatcatctcg aggacttcgg
     1261 gctccaggac gtacacgccg gcgttgatga gcgccgtgcc ctcccactcc ccgggcttct
     1321 ccaggaagct ggcgaccgac ccgtcctcgt ggagctccac caggccgtac cgccgcgggt
     1381 cgtcgaccgg cgtgaggaag atggtgccca tcccgccctt gtccaggtgc gtcgcggcga
     1441 gggcgctcag gtcgacgtcg ctgagcacgt cgccgttgaa gacgtagaag ctgccctcgt
     1501 cgagcaggcc gtcgcagttg gcgatggcgc cggccgtgcc cagcggctct tcctcgaccg
     1561 cgtaggcgac ctcgaggccg tacccggcgc cgtcgccgat cacggcctcg atggcctccg
     1621 cgaggaagcc cgtggagaag atcgcgcgct cgaccccgtg gagggccagg ttgtcgagga
     1681 tgtaggcgac gaacggccgc tccaccaccg gcgccacggg cttgggcacg cgggaggtga
     1741 cggggcgcag ccgcgtgccc tcgccgccga cgaggatgac ggcctgccgc gggatggctc
     1801 gcgccgcgct catgacgggc tcagcgggcg acgggcgcag ggacctcgtc gcccaggccg
     1861 atgccctcgt actcgagccc ggcctccgcc gcgacgagcg ggtcgatgaa gttgcgcccg
     1921 tcgatgagga gcgggcgggc catggtggcc gcggcggcgc tccagtcgag acgcgcgaac
     1981 tcctgccact cggtgaccag taccgccgcg tcggcgccct cgagcgccgc gaggggatcc
     2041 gcgcagatcg cgacgccggg gaacagcggg tgctcgccgg cgtcgagcat ggggtcgtag
     2101 gccatcacct tggcgccctc gtggatgagg cggccggcca gcacgatcga ggccgcctca
     2161 cgcaggtcgt cggtgttggg cttgaacgtg agcccgagca gcgcgatggt ggcgccgcgc
     2221 agcgtaccca gccgctcctt gagcttgccg accacgcggc gcttctgcag cgcgttgacc
     2281 tcgatgaccg cgttgagcag ctggaagtgg tagccggtgt tgcccgccag ctgcttgagc
     2341 gcctgcacgt ccttggggaa gcacgacccc ccgtagccga tgccggcctg caggaagtgc
     2401 gggccgatac ggtggtcgag acccatgccg tgagcgaccg tgcgcacgtc ggccccgacc
     2461 tgctcgcaga cgttggcgat ctcgttgatg aaggagatct tggtggcgag gaacgcgttg
     2521 gaggcgtact tgaccatctc cgccgtaggc acgctggtgc gcaccacgtg ggtgtcgatg
     2581 ccacggtaga ggtcctcgac cgccgctccg tcctcctcgc ggaagctccc gatgacgatg
     2641 cggtccggct cgaggaagtc ctggatggcg gcgccctcgc ggaggaactc cgggttcgac
     2701 gcgtaggtga tgtcgctcgc gcccatcgcc tcgaggtcgg ccgtgatctt gtcgccggtg
     2761 cccacgggca ccgtgctctt catgaccagc agccgcgggc cgccccgcgc cgcgatctcg
     2821 cgcaccgcgt gctgcacgcg cgagaggtcg gcgtcgccgg agggcgaggg gggcgtatcg
     2881 acggcgatga agaggatgcg gcagcgctcg agcagcacgt cggtggacgt ggtgaaggag
     2941 agccgcgccg cgttgtctgt gacgagcttc tcgagccccg gctcgtagat gggcacttca
     3001 ccccgctcga gcctggcgat cttgccttcg tcgacgtcca tgcacacgac gtcgtgcccg
     3061 aggtgagcga agcagacggc cgagacgagg ccgacgtacc cggtaccgac gacccctatg
     3121 ggagcggcat ccatgcggaa cagaacctcc gtgaaaggtg agaaagagcc ccgtttacgg
     3181 gcggaagtct accacgggag gctggcgacc ggcgcgacgc ccgtcgccga cggcctactg
     3241 ggcaagcagc tcgagctcgt agcggctggc ggcgtcgtcg gggttcagcg ccagggcgcg
     3301 cgtgaaggcg gctctcgcag ccttgtcccg cccgagcgtg ccgtgctgga ggacaccgag
     3361 ctcgtaccag acgacgaagt tctgcggctg cagcttcgtc gccgcctgca ggcgttcgag
     3421 cgcctcgcgg ttgcggccga gctgctgcag gagcgtggcc tcggtgatca gggggcccac
     3481 cgcgagcggg tcccacgcgg ccgcccgccg cgcgtggtcc agcgcggtcg cgacgtcgcc
     3541 gtcgctcgag gcggccaggg cggcgttctc ggcgcgcagg gccaggtacg gcggcagcca
     3601 gctggcggcc agcagcagca acgccgcgct ggccaccacg cgcggcgccc agccggagcg
     3661 gcgagcttgc cgcgccggcg tcgtgcgcgc cgtcccggac gcgccgtcct cgccgcgctc
     3721 ccggaccgcc gttccgtcgt cacgcaccgc agcgcgcccg tcgtccgcct cggcgtcccc
     3781 ggccgccgtc cacggcgccg ccgcggcggc tgcctgctcg ggagccgtgg cggtcgcccg
     3841 cgcgactccc tcgtgccgcg acctgcgcgc cgcccgccgc tcg
//
LOCUS       DASUMX010000197         8115 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_212630, whole genome shotgun sequence.
ACCESSION   DASUMX010000197 DASUMX010000000
VERSION     DASUMX010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 8115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8115
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212630"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..912
                     /locus_tag="VFZ86_09785"
     CDS             <1..912
                     /locus_tag="VFZ86_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017623324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5642612.1"
                     /translation="LFLVSVVVFWATQALPGDTARAILGREAANVERYEALKEQLGLN
                     EPLYRQYFDWLGGVLTGDLGNSLAQGTPVTDLLGRRVLNSFILVFIAALVSVPVSLLL
                     GSLTALRRDSKFDVTTTVGSLALAALPEFVIGILLILLFATQVFKVLPAVSRVDSSVP
                     IVQQLELFVLPALTLTLAVAPYITRILRASTIEVLESEYVMMARLKGLPERLVLNRHA
                     VPNALAPALQVTALNLAWLAGGVVVVEYLFAFPGMGSLLVDSVANRDMPMVQAICLII
                     AAVYVLANLTADILTILVSPRLRTGLR"
     gene            909..1832
                     /locus_tag="VFZ86_09790"
     CDS             909..1832
                     /locus_tag="VFZ86_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017592500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5642613.1"
                     /translation="MSTPEIRVNDSELVEPPVVHADRRRSEWLMMLREAWRMGRTKIG
                     VGIFAFIVLIAVAGPWVAPYSPTEFVAPPFSPPETSTMWLGSDYIGHDVLTRVLYGGR
                     TVILLALAATVIGLALGVTLGLITGYARRWIDESVMRILDVVLAFPQIVLTLLFVSIL
                     GPRLWLIVVIVGVSHAPRIARVARAATLEVSQRDFVRAAEAFGIARWKILFNEILPSI
                     TSPLLVEFGLRLTYSIGLIAALSFLGFGMQPPAADWGLMINENRIGIIIQPWPVLMPV
                     LLIGILTIGTNLIADGLGRALIGIERDTGVQ"
     gene            1832..3655
                     /locus_tag="VFZ86_09795"
     CDS             1832..3655
                     /locus_tag="VFZ86_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008029159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5642614.1"
                     /translation="MESATTATTATTEERELVLKVEGLRIVLDPSGVDIDDEVSMEIH
                     RGEVLGLVGESASGKTTVGTSLLDYQRRGAKIAGGSVEIDGHNVLDMDAGKLRSIRGG
                     AISYVPQDSSASLNPALRIGTQLMEILEVHGFGSSQADREARLAQMMAEVLLPSDKAF
                     LRRYPHQLSGGQQQRVAIAMAFANRPKVIVLDEPTTALDVTTQAHVLETVRDLTRSYG
                     VAALYITHDLAVVANLADRIAVMYAGKVVEIGTRDELFDKGHHPYTRKLLASIPDITG
                     ERALRGIPGWAPRPGQRPPGCAFTPRCDWAVDKCTEQFPPVEVVSPGHRVRCWRYKEV
                     GAQGAVLQAETGARTVMETAAEEKQIISVRDVTAYYQHKETLHGITTAVAEGHCLSLV
                     GESGSGKTTLARCIAGLHPHKIVGDIEFMGKPLARDARGRSRAIRQAIQYIFQSPYSS
                     LNPRKTIAQILSQPLKVFFDLSRDEMEDRMVKALEQVALDSSLLSRYPDQLSGGERQR
                     LAIARSLAAEPTVLICDEVTSWLDVSVQAAIIELLAALQRDMNLSMLFVTHNLALVRS
                     ISQEVAVMNDGRIVEYGLVDAVLEDPQADYTKKLLSDTPTV"
     gene            3812..5263
                     /locus_tag="VFZ86_09800"
     CDS             3812..5263
                     /locus_tag="VFZ86_09800"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HEX5642615.1"
                     /translation="MRAHMTWLDEGEKKSFVEEALGILERVGVALKGSAALPVLADLG
                     ASVDTATGVVRFPPDLVLRAVEACPRRVLFAGSSPEYDVVLDEGEPAHFCSSGCAAFV
                     LDHESGVKRPSTLTDLQAATILLDEVPEVDVLWTTITANDVPVEVRELVVCYVVLAEA
                     RKHVTLVDSPSQAEPLLRIMDIVSGDAEAFRARPRFSTLLTVASPLRIDGPLLDFHAA
                     TAARGAPVEVYTVPMAGATSPVTLAGTIVQGLAEFLAVATALQALAPGARAVMGVSGA
                     TMDMRSAGISYAAPECALMNVACVELAHHLGVPAAVPGLATDAKYTGVQAGYEKALKG
                     LATAGVGADVLSGGVGMIDSVNTLFLPQIVVDAEVIGMIRRVLGEVDTGRDEMLLDMI
                     ERVGIGGDYLKEKETARRLRAGVHFAPAISTRQAYDQWVADGRDEVARARERMDVLFA
                     GRAERGRPLADDTLAELAGACGVTPGMARELGH"
     gene            5454..6827
                     /locus_tag="VFZ86_09805"
     CDS             5454..6827
                     /locus_tag="VFZ86_09805"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX5642616.1"
                     /translation="MADVPHTSEQGSLDADMANHLWPPFTQMQGLKPTVMDRAEGALV
                     WDTDGNEYIDAFASLWTVNVGHGRKEIHDAISAQMEKLSIYHIFQIGNEPAIRLAAKV
                     AEHMPGDLNHVFLTLGGGESVETAVKMARQYWRNKGKGTKYMVMFRDRSYHGTTMTSV
                     SAQGLTVNRAKFEPLLPGFIKLGAPYCYRCAWNKTYGEDCRMECATQVAQEIAFYGPE
                     NVGTMLGETMIGTGGVIPPPPEYWPMVNEICKANDVLVINDEVITGFGRSGTWFGFEQ
                     FGSDPDLITLAKGMSSGYLPLGAVGAKDHVFDAFLGGPGEAFMSGATYMGHPLCCAAG
                     IANLEIVEREKLVERCAELGPYLQDGLATLLAHPIVGDVRGVGLVAGIEYVKDKETRE
                     WFAPAQGVAAKIRDEAFRRGVFVRLLGGGHVHAVAPPFIISKEQIDTVVRVLDESIGV
                     VEKDLGY"
     gene            6868..7887
                     /locus_tag="VFZ86_09810"
     CDS             6868..7887
                     /locus_tag="VFZ86_09810"
                     /EC_number="1.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HEX5642617.1"
                     /translation="MGKPRVGVVGYGVIGQRLADGVALQQDMELAGVVDVAPTLSTRA
                     LCEAGMPYDLYVVEAGQKAAFDAAGIPVSGTMDDLLGKVDIVLDSSPGGVGRKNKELY
                     QAKGVKAVFQGGEKNDIVDVFFHGYANYEKGVGKDYLKLTSCNTTGLIRAVDCLDREV
                     GVERVIVTIVRRVADPGDTHRGLVDVAQVEPVPNHQAVDLMTIMPHIEATGLLVHVPT
                     THGHIINVIATTKGDVSKERALELFEAHPRIRNVKIADGFNSNAALFRYARDLGNKRA
                     DMYDIAVFDETIAKSGREFMFAINIPQESVVIPETVDAIRAALSLQTDRLEAVGLTNK
                     YLGLA"
     gene            7929..>8115
                     /gene="pgk"
                     /locus_tag="VFZ86_09815"
     CDS             7929..>8115
                     /gene="pgk"
                     /locus_tag="VFZ86_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010263561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEX5642618.1"
                     /translation="MKYGINTLDDFDWRGKVVLCRVDINAPLDKATGGLRDVTRLEGC
                     APTVRELAEAGARVVLLA"
BASE COUNT         1244 a         2896 c         2767 g         1208 t
ORIGIN      
        1 ctgttcctgg tgtcggtggt cgtcttctgg gccacgcagg ccctccccgg ggacaccgcc
       61 cgcgcgatcc tcggccggga ggcggcgaac gtcgagcgct acgaggcgct gaaggagcag
      121 ctcggactca acgagcccct ctatcggcag tacttcgact ggctcggcgg cgtcctcacc
      181 ggcgacctcg gcaactcgct cgcgcagggc acgccggtca ccgatctgct cgggcggcgc
      241 gtcctcaact ccttcatcct ggtgttcatc gcggccctcg tgagcgtgcc cgtctctctg
      301 cttctgggct ccctgacggc gctgcgacga gactcgaagt tcgacgtcac cacgaccgtc
      361 ggcagcctgg cgctggcggc cctgccggag ttcgtgatcg gcatccttct gatcctcctg
      421 ttcgcgaccc aggtgttcaa ggtgttgccg gcggtctcgc gcgtggactc cagtgtgccg
      481 atcgtccagc aactcgagct gttcgtcctg ccggcgctca cgctgaccct tgcggtggcg
      541 ccgtacatca cccgcatcct gcgcgccagc accatcgagg tgctggagag cgagtacgtg
      601 atgatggcgc ggctgaaggg gctgccggag cggctggtgc tcaaccgcca cgccgtcccc
      661 aacgcgctgg ctccggcgct ccaggtcacg gcgctcaacc tcgcgtggct ggccggcggc
      721 gtcgtcgtgg tcgagtacct cttcgccttc cccggcatgg gctcactgct cgtcgattcc
      781 gtggccaacc gtgacatgcc gatggtgcag gccatctgcc tcatcatcgc cgcggtgtac
      841 gtgctcgcca acctgaccgc cgacatcctc acgatccttg tcagcccacg actgaggacg
      901 ggtctgcgat gagtactcca gagatcagag tcaacgactc cgagctcgtc gagcctcccg
      961 tcgtccacgc cgaccgcaga cggtccgagt ggctgatgat gctgcgtgag gcgtggcgca
     1021 tggggcgcac caagatcggc gtcggcatct tcgccttcat cgtcctgatc gccgtggccg
     1081 gcccctgggt ggccccctac tcgcccacgg agttcgtcgc gccgccgttc tcgccgccgg
     1141 agacgtccac gatgtggctg ggcagcgact acatcggcca cgacgtgctc acgcgcgtgc
     1201 tctacggcgg gcgcaccgtc atcctgctcg ccctcgcggc caccgtcatc ggcctcgctc
     1261 tcggcgtgac cctggggctc atcaccgggt acgcgcggcg ctggatcgac gagtccgtca
     1321 tgcgcatcct cgacgtcgtg ctcgccttcc cgcagatcgt cctcacgctg ttgttcgtct
     1381 ccatcctcgg cccgcgactg tggctcatcg tggtcatcgt cggggtgtcg cacgcgccgc
     1441 gcatcgcccg cgtcgcccgc gccgcgacgc tcgaggtctc gcagcgcgac ttcgtgcgcg
     1501 ccgccgaggc gttcggcatc gcgcgctgga agatcctctt caacgagatc ctgccgagca
     1561 tcaccagccc gctgctggtc gagttcggcc tgcggctgac ctactccatc ggcctcatcg
     1621 ccgcgctgtc gttcctcggg ttcggcatgc agccgccggc ggccgactgg ggcctgatga
     1681 tcaacgagaa ccgcatcggc atcatcatcc agccgtggcc cgtcctcatg ccggtgctgc
     1741 tcatcggcat cctgacgatc ggcaccaacc tgatcgcgga cggcctcggc cgcgccctga
     1801 tcggcatcga gcgcgacacg ggggtgcagt gatggagtcg gcgaccacgg cgacgacggc
     1861 cacgaccgag gagcgcgagc tcgtcctcaa ggtcgagggc ctgcgcatcg tcctcgaccc
     1921 ctcgggcgtc gacatcgacg acgaggtctc catggagatc caccgcggcg aggtgctcgg
     1981 tctcgtcggc gagtcggcct ccggcaagac caccgtgggc accagcctgc tcgactacca
     2041 gcgccgcggc gccaagatcg ccggcgggag cgtcgagatc gacggacaca acgtgctcga
     2101 catggacgcc gggaagctgc gcagcatccg cggcggcgcg atcagctacg tgccgcagga
     2161 ttcgtccgcg tcgctcaacc cggcgctgcg catcgggacg cagctcatgg agatcctcga
     2221 ggtgcacggc ttcggctcgt cgcaggcgga ccgcgaggct cggctggcgc agatgatggc
     2281 cgaggtgctg ctgccctccg acaaggcgtt cctgcggcgc tatccgcacc agctctcggg
     2341 cggccagcag cagcgcgtcg ccatcgccat ggcgttcgcc aaccggccca aggtgatcgt
     2401 gctcgacgag ccgaccaccg ccctcgacgt gaccacgcag gcgcacgtgc tcgagaccgt
     2461 ccgcgatctc acgcgctcgt acggcgtggc ggcgctctac atcacccacg acctcgcggt
     2521 ggtcgccaac ctggccgacc gcatcgccgt gatgtacgcc ggcaaggtcg tggagatcgg
     2581 tacgcgcgac gagctgttcg acaaggggca ccacccctac acgcgcaagc tcctggcgtc
     2641 catcccggac atcaccggcg agcgggccct gcgcggcatc cccggctggg cgccgcgtcc
     2701 ggggcagcga cccccgggtt gcgcgttcac cccccgttgc gactgggcgg tcgacaagtg
     2761 caccgagcag ttcccgccgg tcgaggtggt gagccccggc catcgcgtgc gctgctggcg
     2821 ctacaaggag gtcggagcgc agggggccgt gctccaggcc gagaccggcg cgcgcacggt
     2881 catggagacc gcggccgagg agaagcagat catcagcgtg cgagacgtga ccgcctacta
     2941 ccagcacaag gagaccctgc acggcatcac caccgccgtc gccgagggcc actgtctctc
     3001 gctggtcggg gagtcggggt ccggcaagac gaccctggca cgctgcatcg ccggcctgca
     3061 tccgcacaag atcgtcggcg acatcgagtt catgggcaag ccgctggcgc gcgacgcgcg
     3121 tggccggtcg cgcgccatcc ggcaggcgat ccagtacatc ttccagagcc cctacagctc
     3181 gctcaacccg cgcaagacga tcgcccagat cctgtcgcag ccgctcaagg tcttcttcga
     3241 tctctcgcgc gacgagatgg aggatcggat ggtcaaggcg ctcgagcagg tggctctgga
     3301 ctcgtccctg ctcagccgct acccggacca gctgtccggc ggcgagcgcc agcgtctggc
     3361 catcgcgcgc agcctcgcgg ccgagccgac ggtcctgatc tgcgacgagg tcacctcgtg
     3421 gctggatgtg tcggtgcagg cggcgatcat cgagctcctc gccgccctgc agcgcgacat
     3481 gaatctctcc atgctgttcg tcacccacaa cctcgccctg gtgcgcagca tctcgcagga
     3541 ggtcgccgtc atgaacgacg gccgcatcgt ggagtacggg ctcgtggacg ccgtgctcga
     3601 ggacccgcag gccgactaca ccaagaagct gctgtcggac acgccgacgg tgtaggcggc
     3661 tccggcggcg ggcggacgca acctcgcccg ccgccggaac gcctccccgc cgcacctgcc
     3721 ggccgccccg gcttcgcggc gccccgcgca cgaagcctca cggcgatcag acccatggaa
     3781 gcgacctgag caaggagatc ggaggaggcc gatgcgtgcc cacatgacct ggctggacga
     3841 gggcgagaag aagtcgttcg tcgaggaggc gctgggcatc ctcgagcgcg tcggcgtcgc
     3901 gctcaagggg tccgcggccc tgccggtgct cgccgacctg ggggcctccg tcgacaccgc
     3961 cacaggcgtg gtgcgcttcc cgcccgacct ggtgctccgc gccgtggagg cgtgcccccg
     4021 ccgcgtgctc ttcgcgggct cgtcgcccga gtacgacgtg gtgctggacg agggcgagcc
     4081 ggcgcacttc tgctcctcgg gctgcgccgc cttcgtgctc gaccacgaga gcggcgtcaa
     4141 gcgcccctcc accctgaccg acctgcaggc ggcgacgatc ctgctggacg aggtgcccga
     4201 ggtcgacgtt ctgtggacga cgatcaccgc caacgatgtg ccggtcgagg tccgcgagct
     4261 cgtcgtctgc tacgtcgtgc tcgccgaggc gcgcaagcac gtgacgctcg tcgacagccc
     4321 gtcgcaggcg gagccgctcc tgcgcatcat ggacatcgtc tccggcgacg ccgaggcgtt
     4381 ccgcgcgcga ccgcggttca gcacgctgct caccgtcgcg tcgccgctgc gcatcgacgg
     4441 cccgctcctc gacttccacg cggcgacggc cgcgcgcggc gcccccgtgg aggtgtacac
     4501 ggtgccgatg gccggggcga cctcgccggt gacgcttgcg gggaccatcg tccaggggct
     4561 cgccgagttc ctcgccgtcg ccaccgcgct gcaggcgctg gcgccgggtg ctcgcgcggt
     4621 catgggcgtc tcgggggcca ccatggacat gcgctcggcc ggcatctcct acgccgcgcc
     4681 cgagtgcgcg ctgatgaacg tggcctgtgt cgagctcgcg caccacctcg gcgtgccggc
     4741 ggcggtgccg gggctcgcga ccgacgcgaa gtacaccggc gtgcaggccg gctacgagaa
     4801 ggcgctcaag ggcctggcga cggccggcgt cggcgccgac gtgctgagcg gtggggtcgg
     4861 gatgatcgac tcggtcaaca cgctcttcct gccacagatc gtggtggacg ccgaggtgat
     4921 cgggatgatc cggcgcgtgc tcggcgaggt cgacacggga cgcgacgaga tgctgctcga
     4981 catgatcgaa cgcgtgggca tcggcggcga ctatctcaag gagaaggaga cggcgcggcg
     5041 gctgcgggcg ggggtgcact tcgcccccgc gatctcgacg cgccaggcct acgaccagtg
     5101 ggtcgccgac gggcgcgacg aggtggcgag agccagggag cgcatggacg tgctcttcgc
     5161 cggccgcgcc gagcgcggcc ggccgctggc cgacgacacg ctggcggagc tcgccggcgc
     5221 gtgcggggtc acgcccggca tggcgcgtga gctgggccac tgatccgccg gcgcgtgcgg
     5281 gggtactggc gctcacccac ctgggtggat atactcatct gaccccgtgg gctacacgag
     5341 gtgcgagacc gcgaggaccc tcgcgggtgg actggtggag cagcgccggc gccggcggaa
     5401 gccgggccgg ccggaccgat gcgaccgtcg ccggcggtcg caagggaggc gcaatggcag
     5461 acgtgccgca cacttctgag caggggtcgc tcgacgccga catggcgaac cacctgtggc
     5521 cccccttcac ccagatgcag ggcctcaagc cgaccgtcat ggaccgcgcc gagggtgcgc
     5581 tggtctggga caccgacggc aacgagtaca tcgacgcgtt cgccagcctc tggaccgtga
     5641 acgtcggcca cgggcgcaaa gagatccacg acgcgatcag cgcgcagatg gagaagctct
     5701 cgatctacca catcttccag atcggcaacg agccggcgat caggctcgcc gccaaggtgg
     5761 ccgagcacat gcccggcgac ctcaaccacg tgttcctcac gctcggcggg ggtgagtcgg
     5821 tcgagaccgc cgtcaagatg gcgcggcagt actggcgcaa caagggcaag ggcaccaagt
     5881 acatggtcat gttccgcgac cgctcctacc acggcacgac catgaccagc gtgagcgccc
     5941 agggcctcac cgtgaaccgc gccaagttcg agccgctgct gccgggcttc atcaagctcg
     6001 gcgcgcccta ctgctaccgc tgcgcctgga acaagaccta cggcgaggac tgccgcatgg
     6061 agtgcgccac gcaggtggcc caggagatcg ccttctacgg ccccgagaac gtcggcacca
     6121 tgctcggcga gaccatgatc ggcaccggcg gcgtcatccc gccgccgccg gagtactggc
     6181 ccatggtcaa cgagatctgc aaggccaacg acgtgctggt gatcaacgac gaggtcatca
     6241 ccggcttcgg ccgcagcggc acctggttcg gcttcgagca gttcggctcc gaccccgacc
     6301 tgatcaccct ggccaagggc atgtcctccg gctacctgcc gctgggcgcc gtgggcgcca
     6361 aggaccacgt gttcgacgcc ttcctcggcg gtcccggcga ggcgttcatg agcggcgcca
     6421 cctacatggg ccacccgctc tgctgcgccg ccggcatcgc caacctcgag atcgtcgagc
     6481 gcgagaagct ggtcgagcgc tgcgccgagc tcggcccgta cctgcaggac ggcctcgcga
     6541 cgctgctcgc gcacccgatc gtgggcgacg tgcgcggcgt cggcctcgtg gccggcatcg
     6601 agtacgtcaa ggacaaggag acgcgcgagt ggttcgcgcc ggcgcagggc gtcgccgcga
     6661 agatccgcga cgaggcgttc aggcgcggcg tcttcgtgcg cctgctcggc ggcggccacg
     6721 tgcacgcggt cgcgccgccg ttcatcatca gcaaggagca gatcgacacc gtcgtgcgcg
     6781 tgctggacga gtcgatcggc gtcgtcgaaa aggacctcgg gtactagtcg ccgctgcggc
     6841 ggcatgagac ttcaaggagc caggatcatg ggcaagccaa gagtgggtgt ggtgggctac
     6901 ggcgtcatcg gccagcggct ggcggacggc gtggcgctgc agcaggacat ggagctggcc
     6961 ggcgtcgtcg acgtcgcgcc gacgctctcg acgcgggcgc tctgcgaggc cggcatgccc
     7021 tacgacctct acgtggtcga ggccggccag aaggccgcct tcgacgcagc cggcatcccg
     7081 gtcagcggca ccatggacga cctcctcggc aaggtcgaca tcgtgctcga ctcctcgccg
     7141 ggcggtgtcg gccgcaagaa caaggagctc taccaggcca agggcgtgaa ggccgtgttc
     7201 cagggcggcg agaagaacga catcgtcgac gtcttcttcc acggctacgc caactacgag
     7261 aagggcgtcg gcaaggacta cctcaagctg acgagctgca acaccaccgg cctcatccgc
     7321 gccgtggact gcctcgaccg cgaggtcggc gtggagcgcg tgatcgtgac catcgtgcgt
     7381 cgcgtggccg accccggcga cacgcaccgc gggctcgtcg acgtcgccca ggtggagccc
     7441 gtgccgaacc accaggccgt cgacctcatg acgatcatgc cgcacatcga ggccaccggc
     7501 ctgctcgtgc acgtgcccac cacgcacggc cacatcatca acgtgatcgc caccaccaag
     7561 ggcgacgtga gcaaggagag ggcgctcgag ctcttcgaag cgcacccgcg catccgcaac
     7621 gtcaagatcg ccgacggctt caacagcaac gcggcgctgt tccgctacgc ccgcgacctc
     7681 ggcaacaagc gcgccgacat gtacgacatc gccgtgttcg acgagaccat cgccaagagc
     7741 gggcgcgagt tcatgttcgc catcaacatc ccgcaggagt cggtcgtgat ccccgagacc
     7801 gtggacgcga tccgcgccgc gctcagcctg cagaccgacc ggctcgaggc cgtgggcctc
     7861 accaacaagt acctcgggct cgcctgaacg gtaccgacgg ccggcaagcg ggctgagggg
     7921 gcgcagcggt gaagtacggc atcaacacgc tggacgactt cgactggcgc ggcaaggtgg
     7981 tgctgtgccg cgtggacatc aacgcgccgc tggacaaggc gaccggcgga ctgcgcgacg
     8041 tgacgcggct cgagggctgc gcgccgaccg tgcgggagct cgccgaggcc ggcgcaaggg
     8101 tcgtgctgct cgctc
//
LOCUS       DASUMX010000198         4328 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_500579, whole genome shotgun sequence.
ACCESSION   DASUMX010000198 DASUMX010000000
VERSION     DASUMX010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4328
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500579"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(266..886)
                     /locus_tag="VFZ86_09820"
     CDS             complement(266..886)
                     /locus_tag="VFZ86_09820"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5642619.1"
                     /translation="MSEEGERRRGREATTAVILDAARELFSERGYASVTVRDIGERAG
                     VSHALVHQYIGSKADIFRSVLRRGEGVLASAAPDTPDLLESARLILRRGLEEPGRSHA
                     RLLMGSALSGIPYDRTAGRFAAIELLIELAERQARSASEAERAERDLDPRLVVACVGS
                     LFLGWISGAPWMRPAAGLQDMDEAELADGLERVILSILRDHVPGVA"
     gene            965..2278
                     /locus_tag="VFZ86_09825"
     CDS             965..2278
                     /locus_tag="VFZ86_09825"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX5642620.1"
                     /translation="MAALTTIVAIIILWGVVSRPLDRRGITSAMVFVGAGFLVGTSVL
                     GLLDVSVEGEAAELVTEVALVLLLFSDAARLDLGALRHELRWPTRLLLIGLPLTMLAG
                     TGVGVLVFPGMALASVFLLSTMLCSTDAALGQKVVTDVSVPARVRQALDVESGLNDGL
                     AVPFFLVALDLANAELTTGITWAVVSNAAVQIGWGLAAGVAAGLLGALLFRAADKRAW
                     IGREWRQIVPLAAALLAYALAGTLGGSGFIAAFVGGMVFGAVSGAPGSAVTLFTEETG
                     SLLAAVTWMGFGAIALGLVAPNVTWQVVVYAALSLTLVRMVPVALALLGTGARRPTMT
                     FIGWFGPRGLASVVFALLVLERGVPEGETLLATVVVTVALSVLLHGLTSAPFVRIYHR
                     WYDAHAEAHPEAAEAEAATMPRRRLHLQAADLERLGRVQDEGERR"
     gene            2302..3000
                     /locus_tag="VFZ86_09830"
     CDS             2302..3000
                     /locus_tag="VFZ86_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642621.1"
                     /translation="MERSDRTSPAFKSAAERGLTTPRAAGVAGIIFSLFFVVALALLA
                     EVVGVGLDTETVMANLEGSNSTLGLIGLYLVPFAGIAFLWFIAVVRDRIGVHEDRFFA
                     TAFLGSGLLFVAMLFAAAAVLGGLIAGNSLGTAAPTDIATVGFARSVGYSFLFIYSAK
                     SAGVFVLATSTIIRRSGWPRWASISGFIVAAVLILSVSFFEPIVMLLPAWVSAISIYV
                     LVTGGRRPVGVPSS"
     gene            3265..>4328
                     /locus_tag="VFZ86_09835"
     CDS             3265..>4328
                     /locus_tag="VFZ86_09835"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX5642622.1"
                     /translation="MSKQPNILMIVTDQEYAHQSLPAGFVLSNRDRIHSRGVTFVNHH
                     ATTTVCTPSRSVMYTGRHAPHTGMFDNTNFAWIDDMRADPEALPTIGHMLRDLGYHTA
                     YKGKWHLSELPAPGSREAMEPYGFSEYQDWGDAIGGPMDGLSKDPKTSDEAVDWLTKR
                     APEVAATQPWYMAVNFVNPHDVMFYDTDGEETVQANAMFPIFDAPDTPLYNQKWQTAL
                     PGSFFDDLEGQPPAVRNYERLCDVYYGRIPMDRRDMWHNHVNYYLNCLLDVDRHVGAV
                     LDALEASGQADDTIIIFTSDHGEMGGAHHLRQKGSVAVRETVNVPLVIVDPRHPGGGR
                     TDAVGSHLDLAPTVLAYAGLP"
BASE COUNT          630 a         1483 c         1469 g          746 t
ORIGIN      
        1 gggcgcgaag acgatgccga ggatgggcca cagcgccgag ccgaaggcgg cgtcccagcg
       61 ccccggctgg gcgatccacc agacgatgtc gatggcgcgc gcgccgagca gcaggaggat
      121 ggtgaagaga cagcacatgg gtggctccga tctgggtcgt ggagggagcg caggcgactg
      181 cctcgccttg cccgagcggt ggaagcgcgt cggccacaac ttaccatcgg gtcacactgc
      241 gacgcaagca ttggtccggc gtctcctagg cgacgcccgg cacgtggtct ctcaggatgc
      301 tgaggatcac tcgctcgagc ccatcggcga gctcggcctc gtccatgtcc tggagtcccg
      361 cggccggccg catccagggc gcaccggaga tccagccgag gaagaggctc ccgacgcagg
      421 cgaccacgag gcgcgggtcg aggtcccgct cggcacgctc ggcctcggac gccgacctcg
      481 cctgccgctc ggcgagctcg atgagcagct cgatcgccgc gaaccggccg gcggtgcgat
      541 cgtacggtat gccgctgagc gccgatccca tgagcaggcg ggcgtgagac cggccgggct
      601 cttcgagtcc gcgccgcagg atgaggcgcg cgctttcgag caggtccggg gtgtccggcg
      661 ccgccgacgc caagacgccc tcgccgcgtc tgaggacgga gcgaaagatg tcggccttgc
      721 tgccgatgta ctgatgcacg agcgcatgcg acaccccggc gcgctcaccg atgtctcgaa
      781 cggtgacgga ggcgtacccg cgctccgaga agagctcgcg tgccgcgtcg aggatgaccg
      841 ccgtggtcgc ttcccttccg cgtctgcgct cgccctcctc gctcatccag tcccccttgc
      901 tcgtatctga ccctctggta acatctgccg cagcttcgtc gagcccgtcg ggagcagaga
      961 cacaatggcc gcactgacga cgatcgtcgc catcatcata ctgtggggcg tcgtctcgag
     1021 accccttgac cgccgtggca tcacctcggc gatggtcttc gtgggcgccg gcttcctcgt
     1081 cgggacctcg gtgctcggac tgctggacgt gtccgtcgag ggcgaggcag ccgagctcgt
     1141 cacggaggtg gcgctcgtcc tcctcctgtt cagcgacgcc gcccggctcg acctgggggc
     1201 gctgcgccac gagctcaggt ggccgacgcg cctgctcctc atcgggctgc ccttgacgat
     1261 gctggcgggg acgggtgtcg gcgtgctggt gttccccggc atggcgctcg cctccgtgtt
     1321 cctcctctcc accatgctct gctccaccga cgcggcgctc gggcagaagg tcgtcaccga
     1381 cgtatcggtg ccggcgcgcg tccggcaggc gctcgacgtg gagagcgggc tcaacgacgg
     1441 cctcgccgtc ccgttcttcc tcgtcgcgct cgacctcgcc aacgccgagc tcacgaccgg
     1501 gatcacgtgg gccgtggtca gcaacgccgc cgttcagatc ggttgggggc tcgcggccgg
     1561 ggtcgccgcg ggcctgctgg gcgcgctgct gttccgcgcc gcggacaagc gcgcctggat
     1621 cggcagggag tggcgccaga tcgtgccgct cgccgctgcg ctgctcgcct acgccctggc
     1681 gggcacgctc ggcggcagcg gcttcatcgc cgccttcgtc ggcggcatgg tcttcggggc
     1741 ggtctcgggg gcgcccggct ccgccgtcac gctgttcacg gaggagaccg gcagcctgct
     1801 cgccgcggtc acctggatgg ggttcggcgc catcgcgctc ggcctggtcg ccccgaacgt
     1861 cacctggcag gtcgtcgtgt acgcggcgct cagcctcacg ctcgtgcgca tggtcccggt
     1921 cgcgctcgcc ctgctgggca cgggcgcccg gcggcctacg atgacgttca tcggctggtt
     1981 cgggccgcgg gggctcgcct cggtcgtgtt cgcgctcctg gtgctggagc ggggcgtgcc
     2041 ggagggggag accttgctcg ccaccgtcgt cgtcaccgtg gccctgagcg tgctgctgca
     2101 cggcctcacg tcggcgccgt tcgtgcgtat ctatcaccgc tggtacgacg cgcacgcgga
     2161 ggcgcacccg gaggcggccg aggcagaggc ggcgacgatg ccgcgccggc gtctccacct
     2221 gcaggcggcg gatctcgagc gactcggtcg cgtgcaggac gagggagagc gcagatgact
     2281 ccaggaggaa ggacgacgac tgtggaacga tcagaccgca catcgcccgc gttcaagagc
     2341 gccgccgagc gcgggctcac gaccccgcga gcagccggcg tggcggggat catcttctcg
     2401 ctcttcttcg ttgtagcact cgccctgctg gcagaggtgg tgggcgtagg actcgacacc
     2461 gagacggtga tggcgaacct ggaaggctcg aactcgaccc ttgggctgat cggcctgtac
     2521 ctggtgccct tcgcggggat cgccttcctg tggttcatcg ccgtcgtccg cgaccgcatc
     2581 ggcgtgcatg aagacaggtt cttcgccacc gcgttcctcg gcagcggact gctcttcgtg
     2641 gccatgctgt tcgccgccgc ggccgtgctc ggtggcctca tcgccggcaa cagcctcggc
     2701 acggcggcgc ccacggacat cgcgacggtg ggcttcgcgc gctcggtcgg ctactcgttc
     2761 ctgttcatct actcggccaa gtccgccggc gtcttcgtcc tcgccacgtc gaccatcatc
     2821 cggcgctcgg gctggccacg ctgggcctcc atcagcggct tcatcgtcgc cgccgtgctc
     2881 atcctgagcg tgagcttctt cgagccgatc gtgatgctgc tgccggcgtg ggtgtccgcg
     2941 atcagcatct acgtgctggt gacgggaggc cggcgtccgg tcggggtacc gtcctcttga
     3001 tctgcgacga gtcgcatctc tcgtctggat ggggtgagcg gtgacacaag gggggcatgt
     3061 gtggtcagga gtcagtgctg ctgtcccggc ggcctcatgg tgatcggcct gcacggacag
     3121 gaactcgccg ggtttgcccg cgtcgtagtc ttgcgcgtac gctccgaaga gatcgatgat
     3181 ggccgatcgg tcagcggcgt tgagcgaggc gtcgccgggc gcgtttcctg agggtggcgt
     3241 tgcgatgtct ggggaggatg aatcatgagc aagcagccaa atatcctgat gatcgtgacc
     3301 gatcaggaat atgcccacca gtctctgccg gcgggattcg tcttatccaa ccgggatcgc
     3361 atccactccc gcggggtcac gttcgtcaat caccacgcca ccaccacggt gtgcaccccc
     3421 tcccgctcgg tgatgtacac cggcaggcat gcgccgcaca ctggcatgtt cgacaacacg
     3481 aacttcgcct ggatcgacga catgagggcc gatccggaag ccttgcccac catcggtcat
     3541 atgctgcgtg acctgggcta ccacaccgcc tataagggca agtggcacct ctcagagctt
     3601 ccagctccgg gctcccgtga agccatggaa ccctacggct tctccgagta ccaggattgg
     3661 ggcgacgcga taggcgggcc catggatggg ctctcgaaag accccaagac ttcggatgag
     3721 gccgtcgact ggctgacgaa gcgtgccccc gaggtcgctg cgacccagcc gtggtacatg
     3781 gccgtgaact tcgtcaaccc tcacgacgtg atgttctacg ataccgacgg cgaagagacg
     3841 gtccaggcca acgccatgtt cccgatcttc gacgcgcccg atacgccgct ctacaaccag
     3901 aaatggcaaa cggccttgcc gggctccttc ttcgacgacc tggagggaca gccgccggcc
     3961 gtccgcaatt acgagcgcct gtgcgacgtg tactacggcc ggatcccgat ggatcgccgg
     4021 gacatgtggc acaaccatgt caactactac ctcaactgcc ttctcgacgt agaccggcac
     4081 gtcggagcag tgctggatgc tctggaggct tccggccagg ccgatgacac gatcatcatc
     4141 ttcacctcgg accacggcga gatgggcggc gcccaccacc tgcgccaaaa aggcagcgtg
     4201 gctgtccggg aaaccgtcaa cgtgcctctg gtgatcgtgg atccgcgcca ccctggcggg
     4261 ggcaggacgg atgcggtggg ctcccatctc gacctggctc ccaccgttct ggcgtatgcc
     4321 ggcctgcc
//
LOCUS       DASUMX010000199         3284 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_500637, whole genome shotgun sequence.
ACCESSION   DASUMX010000199 DASUMX010000000
VERSION     DASUMX010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3284
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500637"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..513
                     /locus_tag="VFZ86_09840"
                     /pseudo
     CDS             1..513
                     /locus_tag="VFZ86_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012183971.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
     gene            complement(645..1223)
                     /locus_tag="VFZ86_09845"
     CDS             complement(645..1223)
                     /locus_tag="VFZ86_09845"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5642623.1"
                     /translation="MRKRLNADRRDELLDGVMRIIAERGFSQVTIAEIARELQCSAAT
                     LYKIAPSKDSLVLFAIARWADVTFADIEARSGKGETASERARAYFRAGAERTRPLSLA
                     FYADIGRYESTRLAWWTSVVERYIDRFVELVVSAEEAGEIRSVNAHLLAEVLRQIGTV
                     TRNDDVLRASGLTHEEAVLEVDSLIWEGLLRT"
     gene            1392..1781
                     /locus_tag="VFZ86_09850"
     CDS             1392..1781
                     /locus_tag="VFZ86_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006172624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX5642624.1"
                     /translation="MTKTVAEMIGEAKARIEDVSPKDAADAMAAGEAVFLDVREPVEW
                     EHHVEGAVQVPRGLLEFAADPTSPRYKAELNPAGRVIVYCRSGARAALACATLKDMGY
                     QDVANLSGGFAAWQEAGLPVNEHHDGF"
     gene            1840..3006
                     /locus_tag="VFZ86_09855"
     CDS             1840..3006
                     /locus_tag="VFZ86_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5642625.1"
                     /translation="MAETERVSTTVPLDWSEVWFTNCPMVSANNVDQELGWCREEYKK
                     IGVEYAYFRHAPENNWYPHYIHNLDNLIRMGGLYPPIHVHADLRRTVLLGATWVYEGG
                     CMAVRARDDIFKMSDLKGKKIGISKSLNTIKNDWWRIQEHMGILNMLKLNDMTMDDIE
                     LVEFPYPDDWYDKPEMLQVCMNNPSELWMRRDHKHDLAFRPLENALLGGKVDAIYTQS
                     KVFQHLQEATGKIKMIEDLTRYPDWTVQVANTPAVLTCTDVMADEHPKLVVAYMKAMV
                     KVGRWANENKHAAGAILNRQTFYLDAEDTYQGIKDVDMVPSLSAQNMECVRIGKDFMF
                     EHGYIQRDFDVDEWARPEFLEKAAEEVLKEEWERRSWSKLPHGGGLEVEGTRLG"
BASE COUNT          601 a         1107 c         1087 g          489 t
ORIGIN      
        1 tggagcggca tcgtcgacaa ggcgaaggcc aaggccgtcg ccagacacct catgtcaccg
       61 gcgatgtact cgggctgggg cgtgaggacg cttgccgtcg gcgaagcccg ctacaacccg
      121 atcggctacc acgtcggcac cgtgtggccc ttcgacagct ccatcgtcgc gtggggcctc
      181 cgccgctacg gcttcaagga ggaggcggcg cgcatcgccg caggcatcct cgacgccgcc
      241 gagttcttcg acggtcgtct gccggaggcg ttcggcggct accccaggga gctgacgaag
      301 tacccggtgc agtacccgac cgcctgcagc ccgcaagcct ggtcgacagg tgcgccgctg
      361 ctcctgctgc ggacgatgct cgggctcgag ccgaccgggg accacctcat cgtcgacccg
      421 gtcctcccga gcggcatcgg ccgtctggaa ctgctcgaca tccccgggcg ctggggcagg
      481 atggacgcct tcggccgggg ccgggtcgac gtcgagaagg ggccccggtc gcggtcgcag
      541 ctcggcgtcg tgccgcacgc ggccaagccg aagcgtcgca agggcggtcg agactcgtcg
      601 ccggacgcgt gatgcgcatc gccgggggga gcagaccgcg ccgcctacgt ccggagcagc
      661 ccctcccaga tgagtgagtc gacctcgagc acggcttcct cgtgggtgag accgctggcg
      721 cgcaggacgt cgtcgttgcg cgtcaccgtc ccgatctgcc ggagcacctc cgcgagaaga
      781 tgcgcgttga ccgagcggat ctcgccggcc tcctccgcgc tcacgaccag ctcgacgaag
      841 cggtcgatgt agcgctcgac gacactcgtc caccaggcca gccgcgtcga ctcgtaccgc
      901 ccgatgtcgg cgtagaaggc gagtgacagc gggcgcgtgc gctcggcgcc ggcgcggaaa
      961 tacgctcggg cccgctccga ggcggtctca cccttgccgg agcgggcctc gatgtccgcg
     1021 aaggtgacgt cggcccagcg cgcgatggcg aacagcacga ggctgtcctt gctgggcgcg
     1081 atcttgtaca gcgtcgcggc ggagcactgg agctcacgcg cgatctccgc gatcgtgacc
     1141 tgggagaagc cgcgttccgc gatgatgcgc atcacgccgt cgagcagctc gtcccggcga
     1201 tccgcgttca gacgctttct cacttcgacg gcctcccagg ccagcgtgcc cactcgttcg
     1261 cccaaggcta tcccagcagc acgagggccg cattccgacg ctcatcatac agactatcgg
     1321 taactagaga agtattgcgt tactctaaat cgtactccat actggcgggc atccgtacca
     1381 gggaggaccc gatgacgaag acggtcgccg agatgatcgg cgaggcgaag gcccggatcg
     1441 aggacgtgtc gccgaaggac gcagcggacg cgatggcagc gggcgaagcc gtgttcctcg
     1501 acgtgcgcga gccggtggag tgggagcacc acgtcgaggg cgccgtccag gtgccgcgtg
     1561 gcctgctcga gttcgcggcc gaccccacga gcccgcgcta caaggcggag ctcaatccgg
     1621 ccggccgcgt gatcgtgtac tgccgctccg gcgcgcgcgc cgccctggcg tgcgccacgc
     1681 tcaaggacat gggctaccag gacgtggcca acctgtcggg cggcttcgcg gcctggcaag
     1741 aagccggcct gccggtgaac gagcatcacg acggtttctg agcaccgccc gcccgcacac
     1801 ctcgaacgca cacgacggaa gacgagagga gatctgacga tggcagaaac ggagagggtc
     1861 agcaccacgg tgccgcttga ctggagcgag gtgtggttca ccaactgccc catggtttcg
     1921 gcgaacaacg tcgaccagga actcggctgg tgccgcgagg agtacaagaa gatcggcgtg
     1981 gaatacgcct acttccgcca cgcccccgag aacaactggt acccgcacta catccacaac
     2041 ctcgacaacc tcatccgcat gggcggcctc tacccgccga tccacgtgca cgccgacctg
     2101 cgccgcaccg tgctgctcgg cgccacatgg gtctacgagg gcggctgcat ggcggtgcgc
     2161 gcccgcgacg acatcttcaa gatgagcgac ctcaagggca agaagatcgg gatctcgaag
     2221 agcctgaaca ccatcaagaa cgactggtgg cgcatccagg agcacatggg catcctgaac
     2281 atgctcaagc tgaacgacat gaccatggac gacatcgagc tggtcgagtt cccctatccg
     2341 gacgactggt acgacaagcc cgagatgctc caggtgtgca tgaacaaccc gtcggagctg
     2401 tggatgcgcc gcgaccacaa gcatgacctc gccttccgcc cgctggagaa cgcgctgctc
     2461 ggcggcaagg tggacgccat ctacacgcag agcaaggtct tccagcacct gcaggaggcc
     2521 acgggcaaga tcaagatgat cgaggacctg accaggtacc ccgactggac cgtccaggtc
     2581 gccaacaccc ccgccgtcct cacgtgcacg gatgtcatgg ccgacgagca ccccaaactg
     2641 gtcgtggcct acatgaaagc gatggtcaag gtcgggcgct gggccaacga gaacaagcac
     2701 gccgccggcg cgatcctcaa ccgccagacc ttctacctcg acgccgagga cacgtaccag
     2761 ggcatcaagg acgtcgacat ggtgccgagc ctgagcgcgc agaacatgga gtgcgtcagg
     2821 atcggcaagg acttcatgtt cgagcacggc tacatccagc gcgacttcga cgtggacgag
     2881 tgggcccggc cggagttcct cgagaaggcg gctgaagaag tgctcaagga ggagtgggag
     2941 cgccggagct ggagcaagct gccgcacggt ggcgggctcg aagtcgaggg cacacggctc
     3001 gggtgatacg caagctcgtg attgtgcgga ccactgaccg tctggtaact tccgttcatc
     3061 tcactgaagc cctcttgggg ggtacggtcg gccggagccg gagggggcaa ctcctgatcc
     3121 gccgcataca ctgattggag tcgcccatgt gctgtctctt caccatcctc ctgctgctcg
     3181 gcgcgcgcgc catcgacatc gtctggtgga tcgcccagcc ggggcgctgg gacgccgcct
     3241 tcggctcggc gctgtggccc atcctcggca tcgtcttcgc gccc
//
LOCUS       DASUMX010000200         6453 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_501036, whole genome shotgun sequence.
ACCESSION   DASUMX010000200 DASUMX010000000
VERSION     DASUMX010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 6453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6453
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501036"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="VFZ86_09860"
     CDS             complement(<1..614)
                     /locus_tag="VFZ86_09860"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HEX5642626.1"
                     /translation="MRIRTVSLVALAVAGVIAVIAIVAAPAFAASPSASAGRSSSVTP
                     VKKPPVVKNYIRYGAGRKAQTADYCRRHYRQHTYWLTDPKVIVLHHTAGSTWQSAWYT
                     FDANTAYNGEKPGVSAQFIVHTDGTIYQCMSLSLRARHAIGMNWKSIGIEFVQEARSG
                     KDGHWMDRQILNRDRQMAAGLRLVRYLQARFGITTANVIGHAMA"
     gene            complement(701..2896)
                     /locus_tag="VFZ86_09865"
     CDS             complement(701..2896)
                     /locus_tag="VFZ86_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015181252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX5642627.1"
                     /translation="MDEGTDQKRAVSPDRRAVAPYGAWRSPVTARLIAEGGVGIMWPQ
                     SVGESLYWTELRPLQDGRYVVVRRAPDGAIADVTPEDISARTLVHEYGGGMYAAFRNE
                     DGGESVLFSDQADQRLYRQDLRPAGAGRSGVDAPADAAAPPGSAAPSAWSAPRPVTPA
                     PPSDRAHRYADGRVTPDGRLLVSVRERHEAGGEVVNELVSLPTDGSAEPRVIASGHDF
                     YAAPRLGPDGRRVAWLSWDHPGMPWDGTELWTAELAADGGVTGERLVAGGPDESVLQP
                     LWSPTGALWLASDRTGWWNLYAVEADPAVGGASGTSAGGRPEPCPLVTRAAEFATVPW
                     VFGLQSYAFLSDGRLLVSFTEDGRDHLAVLDAPEAGAPAADSGADSAAGPDSDPSHEP
                     VALRELDSPFTVVASLTPLGSRAGVVVASHTEGWQVAVLDPDGGEAEVVRRGRHIPVD
                     PAYISRPEPIVFPTEGGLTAHANYYAPANPDFIAPDGELPPLLVHVHGGPTSSHDAKL
                     DLDVQYWTSRGIAVVAVNYGGSTGYGRAYRERLKGEWGVVDTVDAINAARYLIARGDA
                     DPARVGIRGGSAGGYTTLNALTRFDFFSAGASLFGLADLEEFATGGTHKFESQYLESL
                     VGPYPERRDVYRERSPIHHVDDLSCPVILLQGLEDAIVPPHQAEIIVEALRRKDLPYA
                     YLAFEGEQHGFRKAENIIRAEEAELYFYGRAWGFTPADEIEPVVIENLG"
     gene            complement(3100..5466)
                     /locus_tag="VFZ86_09870"
     CDS             complement(3100..5466)
                     /locus_tag="VFZ86_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642628.1"
                     /translation="MRIVARGAVTRTARLVPLLLALLALLAVAQVAAAAPPGPTMDLE
                     QLAVALESGPLDGYLLTTMDGTTPEPITVQVQSLVDYSWGSLILFEASGPWIERIGGI
                     AAGMSGSPIYVDDGGVDKLVGALSYGDAFTLGGLGLATPIEYMAAIESDYAVGALAAL
                     QAPRPPAPGAYPLAEPVKTSAGVVRSVVVARSATAAAGVGATSGQPVMTPLGILEIGG
                     AKPGSKAFERVAARFEKTGLLVRAASGDGRWAGAPTPDLTGGSPCAILFSQGAVWVGA
                     AGTVTYVDGESAMLFGHPFEQLGAIDAILTGGDVQGVWASGYIPYKLIAPRDVKGACV
                     QDRNWGIQARLGQVPDLFPVATTVTIADRAVTVSDDSEVSEWLVTADAYPGLPADIVA
                     QVVWNAVDQYSYPGSAETVTTVTASDHTGTYTIVHENLWSNSWDVSYDTGTDAYWILS
                     ELAADPDGVLHPRVESVDVTTTVSPTQRTARIARVTLPDGIGVGDTTVRIEYYRYGSA
                     ELQTVDAVLVIPAGTPLYGRLVVQPATWGGEDDGEGENTADSAPPRTLAELVDQLNGT
                     PMNSDLIVSYLPGDEGGEGEPGAAASIDATIPTDYVFSSYYSAATARVMIEAQPRRVA
                     YGGAVQVTGIVDASSETTVHIYRRYADSDTAVKVATTTATPVDGMASFAAVVPGLKKN
                     ATLIARTDPMDGTLPGSASVSVKVVAKVWLSGSSRLAIHVRPGEADGTARLQKKRGGV
                     WTAYKTVKITDGEGFTTLPKGTHTLRVRFSGSALCAPGTSRAYTIRVP"
     gene            complement(5614..>6453)
                     /locus_tag="VFZ86_09875"
     CDS             complement(5614..>6453)
                     /locus_tag="VFZ86_09875"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEX5642629.1"
                     /translation="ANRAPNPNFGQALVLGGQQLYGGTSAIEQLFFYTYKYDHVNTFK
                     GAYHINPDYSHWFGWSEVNQDLDLIKGEEAKLRRLAAQYDPGFVVTTEKPTTGQPVTF
                     DASGLAAWGNASANTYEWWTGDGGHAMPAAAAVLSYTYAGAGTYTVELTCADGDLVND
                     AANPAACSGKRTTSLRVTVKDAAKLTLAAPARVEAGRTLTLTGKLTTGSAGDRTVRLQ
                     TQTRSGGWKTAAAKTVTVAAGTAKAVTFKYKLTKTTTFRLSYGGSASVWSAVSQTRKA
                     RPL"
BASE COUNT          823 a         2260 c         2395 g          975 t
ORIGIN      
        1 ttggccatcg cgtggccgat gacgttggcg gtggtgatgc cgaaccgcgc ctgcaggtag
       61 cgcaccagcc gcagtcccgc ggccatctgc cggtcgcggt tcaggatctg ccggtccatc
      121 cagtggccgt ccttgcccga ccgtgcctcc tgcacgaact cgatgccgat cgacttccag
      181 ttcatgccga tcgcgtgacg cgcgcgcagg ctgagcgaca tgcactgata gatggtgccg
      241 tccgtgtgga cgatgaactg cgccgagacg cccggcttct cgccgttgta ggcggtgttg
      301 gcgtcgaacg tgtaccaggc gctctgccag gtcgaccccg ccgtgtggtg gagcacgatg
      361 accttggggt ccgtgagcca gtacgtgtgc tggcggtagt gccgccggca gtagtcggcc
      421 gtctgcgcct tgcgcccggc gccgtagcgg atgtagttct tgacgaccgg cggcttcttc
      481 acgggcgtca cgctcgagga cctccccgcc gaggcactcg gggacgcggc gaacgcgggc
      541 gccgccacga ttgctatgac ggcgatgacg cccgccacgg cgagcgcgac gaggctgacg
      601 gtgcggatgc gcatggcgtt cgagtatacg ccccgccgat tggatcacct tcggggcgcc
      661 gcgaaagggt gaccgtaggg agaccgcatt cggaccctgg tcagcccagg ttctcgatca
      721 ccaccggctc gatctcgtcg gccggcgtga acccccacgc gcgtccgtag aagtacagct
      781 ccgcctcctc cgcgcggatg atgttctccg ccttgcggaa gccgtgctgc tcgccctcga
      841 acgcgaggta ggcgtagggc aggtccttgc gccgcagcgc ctccacgatg atctccgcct
      901 ggtgcggcgg gacgatggcg tcctccaggc cctggagcag gatgaccggg cagctgagat
      961 cgtcgacgtg gtggatgggc gagcgctcgc ggtagacgtc gcgccgctcg ggatagggcc
     1021 cgaccaggct ctcgaggtac tgcgactcga acttgtgcgt gccgccggtg gcgaactcct
     1081 cgaggtcggc gaggccgaag aggctggcgc cggcgctgaa gaagtcgaag cgcgtgagcg
     1141 cgttgagcgt ggtgtagccg ccggcgctgc cgccgcggat gccgacgcgc gcggggtcgg
     1201 cgtcgccgcg cgcgatcagg tacctggcgg cgttgatcgc gtccacggtg tcgacgacgc
     1261 cccactcgcc cttgagccgc tcgcggtacg cgcggccgta gccggtcgac ccgccgtagt
     1321 tgacggcgac cacggcgatg ccgcgcgacg tccagtactg gacgtcgagg tcgagcttgg
     1381 cgtcgtgcga cgaggtgggg ccgccgtgca cgtggaccag taacggcggc agctcaccgt
     1441 ctggcgcgat gaagtcgggg ttcgccggcg catagtagtt ggcgtgcgcc gtgaggccgc
     1501 cctccgtggg gaacacgatc ggctccgggc ggctgatgta tgccgggtcg acggggatgt
     1561 gccggccccg gcgtaccacc tcggcctcgc caccgtccgg gtcgaggacc gcgacctgcc
     1621 agccctccgt gtggctcgcc acgaccactc cggcccggga tccgagcggc gtcagcgagg
     1681 cgaccacggt gaagggcgag tcgagctcgc gcagcgcgac gggctcgtga gatgggtcgg
     1741 aatccggccc cgcggccgag tcggcgcccg agtcggcggc cggggcgccg gcctccggcg
     1801 catcgagcac ggcgaggtgg tcgcgcccgt cctcggtgaa gctcacgagg agccggccgt
     1861 cgctcaggaa cgcgtagctc tgcaggccga agacccaggg cacggtcgcg aactcggcgg
     1921 cgcgcgtgac gagcgggcag ggttcggggc gtccgccggc ggacgtgccg ctcgcgccgc
     1981 cgacggctgg gtccgcctcc accgcgtaga ggttccacca gccggtgcgg tcgctcgcga
     2041 gccagagcgc cccggtgggg ctccagagcg gctgcaggac cgactcgtcc ggcccgccgg
     2101 cgacgagccg ctcgccggtg acgccgccgt cagccgcgag ttccgccgtc cacagctcgg
     2161 tgccgtccca cggcatgccg gggtggtccc acgagagcca ggcgacgcgg cgcccgtccg
     2221 ggccgagccg cggcgccgcg tagaagtcgt ggccggaggc gatgactcgg ggctcggccg
     2281 atccgtcggt ggggaggctc accagctcgt tgaccacctc tccgccggcc tcgtgccgct
     2341 cgcgcacgct cacgagcagg cggccgtccg gcgtgacgcg cccgtcggcg tagcggtggg
     2401 cgcggtccga cggcggggcc ggggtgacgg ggcggggggc ggaccaggcg gagggggcgg
     2461 cggagcccgg cggcgcagct gcgtccgccg gcgcgtccac gccggaccgg ccggcgccgg
     2521 ccggccgcag gtcctgccgg tacaggcgct ggtcggcctg gtcgctgaag aggacgctct
     2581 cgccgccgtc ctcgttgcgg aaggcggcgt acatgccgcc gccgtactcg tgcaccagcg
     2641 tccgcgcgct gatgtcctcg ggcgtgacat cggcgatcgc gccgtccgga gcccggcgca
     2701 cgaccacgta gcggccgtcc tgcagcgggc gcagttcggt ccagtacagg ctctcgccca
     2761 cgctctgcgg ccacatgatg cccacgccgc cctcggcgat gagcctggcc gtcaccgggg
     2821 aacgccacgc cccgtagggc gcgaccgctc gccggtccgg cgacaccgcg cgcttctggt
     2881 cagtcccttc gtccatggat cgtcctcacg gtagacggag aggcgcggcg gtctgggcgt
     2941 gccgccgcgc ctccgggtgc tgcccggccg ggggggtagc aagcggccgg acgcacgtcc
     3001 ttggcagcgc aacgggacgc tgcgcgcgtc ccgttgcctt ttcaagcgcg tcctaccccg
     3061 tgcgccgccg cggcaaccgc gtcgccgtcg cgtaccgtct cacggcaccc ggatggtgta
     3121 cgcgcgggac gtcccgggcg cgcacaacgc gctgcccgag aacctgacgc gcagcgtgtg
     3181 cgtgcccttg ggcagcgtgg tgaagccctc accgtccgtg atcttcacgg tcttgtaggc
     3241 ggtccagacg ccgcccctct tcttctggag cctggccgtt ccgtcggcct cgcccggcct
     3301 cacgtggatg gccagccgcg aggatccgga cagccacacc ttggcgacga ccttgaccga
     3361 gaccgaggcg ctgcccggca gcgtgccgtc catcggatcc gtgcgtgcga tcagtgtcgc
     3421 gttcttcttg agtcccggga ccacggccgc gaagctcgcc atgccgtcca ccggggtcgc
     3481 ggtcgtcgtc gccaccttga ccgccgtgtc cgagtccgcg tagcggcggt agatgtggac
     3541 ggtcgtctcg ctcgaggcgt ccacgatgcc ggtcacctgg acggcgccgc cgtaggcgac
     3601 cctgcgtggc tgtgcttcga tcatgacgcg cgcggtcgcc gccgagtagt agctgctgaa
     3661 gacgtagtcg gtcgggatcg tcgcgtcgat gctggcggcc gcgcccggct cgccttcccc
     3721 gccctcgtcg cccggcaggt acgagacgat gaggtcgctg ttcatcggcg tgccgttcag
     3781 ctggtcgacg agctcggcaa gggtgcgtgg cggcgctgag tccgcggtgt tctcgccctc
     3841 accgtcatcc tcgccccccc aggtcgcggg ctggacgacc aggcgcccgt acagcggcgt
     3901 gcctgcggga atgacgagga cggcgtcgac ggtctgcagc tccgcggagc cgtagcggta
     3961 gtactcgatg cgcaccgtgg tgtcgcccac gccgatgccg tcggggaggg tgacgcgggc
     4021 gatgcgcgcc gtacgctgcg tgggcgagac ggtcgtcgtc acgtccacgg actcgacccg
     4081 cggatggagg acgccgtcgg ggtccgccgc caactcggag aggatccagt aggcgtcggt
     4141 gccggtgtca tacgagacgt cccaggaatt gctccacagg ttctcgtgca cgatggtgta
     4201 tgtcccggtg tggtcggacg cggtcacggt cgtgacggtc tcggcgctgc ccgggtagct
     4261 gtactgatcg accgcgttcc acacgacctg ggcgacgatg tcggccggca gtccgggata
     4321 ggcgtcggcg gtcaccagcc actcgctgac ctcggagtcg tcgctcacgg tcaccgcgcg
     4381 gtcggcgatg gtcaccgtgg tcgcgaccgg gaacagatcc gggacctgcc cgaggcgcgc
     4441 ctggatcccc cagttgcggt cctggacgca ggcgcccttg acgtcgcgtg gggcgatcag
     4501 cttgtagggg atgtagccgc tcgcccacac gccctgcacg tcgccgccgg tgaggatggc
     4561 gtcgatggct ccgagctgct cgaaggggtg cccgaagagc atcgccgact cgccgtcgac
     4621 gtaggtgacg gtgccggcgg cgccgaccca gacggcgccc tgcgagaaga ggatcgcgca
     4681 gggcgacccg ccggtgaggt cgggcgtggg cgcaccggcc catctgccgt cgccgctggc
     4741 cgccctcacc agcaggccgg tcttctcgaa cctggcggcg acgcgctcga acgccttgct
     4801 gcccggcttg gcgccgccga tctcgaggat gccgagcggc gtcatcaccg gctggccgct
     4861 cgtagccccg acgcctgcgg cggcagtggc gctgcgcgcg accacgacgc tgcgcaccac
     4921 gccggccgag gtcttcaccg gctcggcgag cggataggcg ccgggcgccg gcggccgggg
     4981 agcctgcagt gccgccagcg cgccgaccgc gtagtcgctc tcgatcgccg ccatgtactc
     5041 gatcggcgtg gcgaggccga gcccgcccag ggtgaaggcg tcgccgtagg agagggcgcc
     5101 gacgagcttg tcgacccctc cgtcgtcgac gtagatgggg ctgccgctca tgccggcggc
     5161 gatgcccccg atccgctcga tccagggccc ggaggcctcg aagaggatca gcgagcccca
     5221 cgagtagtcg acgaggctct gcacctgcac ggtgatcggc tccggcgtgg tgccgtccat
     5281 cgtcgtgagg aggtagccgt cgagcggacc ggactcgagc gccacggcga gctgctcgag
     5341 gtccatcgtc ggcccgggag gcgcggctgc ggcgacctgg gcgactgcga gcagcgccag
     5401 cagcgccagc agcagcggaa cgaggcgcgc cgtacgtgtg acggcgccgc gggccacgat
     5461 tctcatggat cctccagatg tcgcggcgac ggccgggacg gcgtcgctcc ccggcacagc
     5521 gtacaccgcg gggcaaggcg gctgggaacg gacgggggcg ggcggccgtc acggccgccc
     5581 gccccgcctt gcacgtgctt cagaccaccc cgctcagagt ggtctggcct tgcgcgtctg
     5641 gctgaccgcg ctccacaccg acgcgctgcc gccgtagctc aggcggaacg tcgtcgtctt
     5701 cgtgagcttg tacttgaacg tcaccgcctt ggcggtgccg gcggcgaccg tcacggtctt
     5761 ggcggcggcg gtcttccagc cgcccgacct ggtctgcgtc tgcaggcgca ccgtccggtc
     5821 tccggcggag ccggtggtca gcttgccggt cagcgtgagc gtcctgccgg cctcgacgcg
     5881 ggcgggcgcg gccagcgtga gcttcgccgc gtccttgacc gtgacccgga gcgacgtcgt
     5941 ccgcttgccg gagcacgccg ccgggttcgc ggcgtcgttg acgaggtcgc cgtccgcgca
     6001 ggtgagctcg accgtgtacg tccccgcgcc ggcgtacgtg tacgacagca ccgccgcggc
     6061 cgcaggcatc gcgtggccgc cgtcgccggt ccaccactcg taggtgttgg cggaggcgtt
     6121 gccccacgcg gccaggccgg acgcgtcgaa ggtcaccggc tggccggtgg tgggcttctc
     6181 cgtggtcacg acgaagccgg ggtcgtactg ggcggccagc cggcgcagct tggcctcctc
     6241 gcccttgatc aggtcgaggt cctggttgac ctcggaccag ccgaaccagt ggctgtagtc
     6301 gggattgatg tggtacgcgc ccttgaacgt gttcacgtgg tcgtacttgt aggtgtagaa
     6361 gaagagctgc tcgatcgccg acgtgccgcc gtagagctgc tgtccgccca gcaccagcgc
     6421 ctgcccgaag ttggggttcg gggcgcggtt cgc
//
LOCUS       DASUMX010000201         8007 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_2082547, whole genome shotgun sequence.
ACCESSION   DASUMX010000201 DASUMX010000000
VERSION     DASUMX010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 8007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8007
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2082547"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            198..887
                     /locus_tag="VFZ86_09880"
     CDS             198..887
                     /locus_tag="VFZ86_09880"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX5642630.1"
                     /translation="MTAPQRDAGLPRGPCVPVSVHRLRYAWLAAIGLLLLVVTALWSL
                     GDQPYAAERRAFDLVNGLPEALAYLLIPVMQAGSLAAVFAAGILALIARRPRLAGLVV
                     MAGGSAWVLAKLVKRVVERGRPDEYLTDVVIRGAVQMGQGFPSGHAAVIAAMATVATP
                     YLSRPGRLVVWTVVGLVALARVYTGAHLPLDVVAGVMVGWGFGCVVNLTLGTPAFACC
                     PRSRLDCTVLE"
     gene            975..2705
                     /gene="sulP"
                     /locus_tag="VFZ86_09885"
     CDS             975..2705
                     /gene="sulP"
                     /locus_tag="VFZ86_09885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate permease"
                     /protein_id="HEX5642631.1"
                     /translation="MATRIVRWHRLQPPTLPIRDWLPGYRRRWLRPDLLAAAAVWAVL
                     VPEGIAYASLAGLPPATGLFAALAPLLAYAVLGTCRQLTVGPSSAIAAYSAAAVAPLA
                     LGDDGRFIALSALLALLVGALLLVAGLARAGFVADFFARPVLTGFVAGLALVIGVGQL
                     YKLVGVESGGTTFFGKLEALVSQLGDVNGPTLAIGLTSLAVIFGLRAYAPKAPSALVA
                     VLLGTVAVAVFDLQDYGVAIVGTIPDGLPTPALPSFGLADVIDLLPDAFALALICFAE
                     SVAGARALAAKHRYEVDADQELIALGVSNLGAGLLQGFTVDASLSRSAVADSSGVKSQ
                     LSNIILFCLLVVTMLFLMPLFHDLPEAVLGAIVIAAVAHLVDVGALRRLRRADSTDFV
                     VAVLCFAGVLVFGLLTGLAIAVVLSLLALVYRAYRPSYAILGRAPGAVDDERLRYRGI
                     EDHPDVQTFPGLVILRIDGELFFANARWFRETVRSLVHDQSPPVREVLVHAGAVPHID
                     TTAAAMLKGLIAELREDGVELALARVTTGLLHDLERNGVARLIGDERFFDTVAGGVDD
                     FLHRSQPRLG"
     gene            2768..3985
                     /locus_tag="VFZ86_09890"
     CDS             2768..3985
                     /locus_tag="VFZ86_09890"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX5642632.1"
                     /translation="MIADTPKTAAELGLMRGSPPPPDKRVTFWNWETPPFNRWSFQHV
                     PAFVRTARVWRGDGPVSRFERSPVRLDDLALETPAGEQVTVADVLRRTCTDGFLVLHE
                     GRIVAEEYFNGMTPAGRHLLMSVSKSIVGMLAGIVIGRGALSADDLVTDVVTELRGTS
                     FEQATVRHLLDMRTGTRFSEDYDDPQSDVCRSERVADWGPLLPGEESSGLYGYVPTLG
                     NARDHGGAFEYRSILTDVLGWVLARASGQSLPELLSSALWAPLGAEEDADVTVDRHGC
                     AWVDGGICVTLRDLARLGQMHLQDGWLDGRQIVPAEWVRDTRTGGADSRTAFAASAWG
                     AMLPAAFYRNQFWVVRPEDGVYTGWGIHGQFLYIDVPARVVIAKLSTLPRALDREVWA
                     DQVAALDAIAASL"
     gene            complement(4184..5320)
                     /locus_tag="VFZ86_09895"
     CDS             complement(4184..5320)
                     /locus_tag="VFZ86_09895"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011022857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HEX5642633.1"
                     /translation="MRHGKTIVLVTAALVTLLCVAAPAFAATLTPVEELGKALFFDEN
                     LSLNRDQSCATCHAPEVGWTGPTAAINAHGAVYEGSDPGMFGDRKPPTSAYGGDSPVL
                     HYDEVAGLWIGGMFWDGRATGWTLGDPLAEQAQGPFLNSREQALASSDDVIAAVKLST
                     YAGLFEQVWGPDSLDADVAYDYVGLSIAAYEASSEVNPFSSKYDAWVAGKAKLTRPEM
                     QGFRLFMNKGKCGNCHVPPLFTDFTYDNLGIPKNLENPVYTWNSIYWVDPGLGGFLKS
                     MAEPESVWAAEMGKHKVPTLRNVDLRPSPDFVKAYGHNGYFKSLRDIVHFYNTRDVAV
                     WDPPEYAATVNTTEMGNLRLTAVQEKAIVTFMKTLSDGYLPQGR"
     gene            complement(5861..7072)
                     /locus_tag="VFZ86_09900"
     CDS             complement(5861..7072)
                     /locus_tag="VFZ86_09900"
                     /inference="COORDINATES: protein motif:HMM:NF027964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HEX5642634.1"
                     /translation="MSMRILQLGVGSVGEVTARTVASAPEVSAVVLADLDASRPQAVA
                     GKLPPGKAELLQLDVHDKEALVKALAGVDLVINGLIPEVNLDVMAACLEAGTHYLDMA
                     AAGPRDVVGTADVDEELALSDAFKKAGLTGLVFFGIDPGASDVFARTLYDQLDTVERL
                     MVLDGDASSADGVEFAPSFSPTTMVEECLILPPHAYEDGRKIRRTSLSSKFDFTFPDP
                     VGPLTVWNVDHEESQLMPLFLGDKGLRNADFYIALDEGWANLLLAWRKLGFDHNREIE
                     FGGARFRPLDLLVSRLPRSVDLIGRMHGAVCVGTLAEGSVGGRRVRRFMYQITSHDEA
                     YEKHGVQGTGWQTGVPAACAAIMLARGLAPGPGVFAPEQIDPAPFLELMTAHGTPWGV
                     VDLPPDEELPV"
     gene            complement(7069..>8007)
                     /locus_tag="VFZ86_09905"
     CDS             complement(7069..>8007)
                     /locus_tag="VFZ86_09905"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX5642635.1"
                     /translation="GGSGAALASGTAMLATGSDIGGSIRIPASLCGVVGYKPPYGRVP
                     ALPPFNLDHYCHDGPMARTVEDCRLLENVMAGPHRDDIVSLRPKLTIPEDLPGAEGLR
                     IAMDADLGGFDVDDEVRENLQVAAGVFRDLGAQVEDAAIRLDVGDVREAARAHFAAIF
                     GSLIGRVLPDHRHLMTSYAVAFAEDAAAPTVGFYRSLEIESEVYAQIAGVLEDHDLLV
                     TPVFGVPALKATTADYDIEVMYRRGLTLVFNLCSRCPVLVVPCGRSRDGVPMGIQIVG
                     RTYDDVSVFRAASAFAVARPWFADASLRPAFEGSPT"
BASE COUNT         1068 a         2812 c         2860 g         1267 t
ORIGIN      
        1 gtcctgtctc ggtcgcgcgg tctcttcatg gtggtgcgcg cgccttgagg gacggcgcgg
       61 attcgagggt gtgcggtcct gacagcacca tccccgggat ccgggaggac aaccacgacc
      121 gcgtttactc ccgtggccgg ctggctaggt cacaagagac acgggcgccc gcgcgcccac
      181 ccacggaggg agcgagcatg acggcaccgc agcgagacgc cggcctcccg agggggccgt
      241 gcgtccccgt cagcgtgcat cgcctgcgtt acgcgtggct ggcggccatc ggtctcctgc
      301 tgctcgtggt caccgccctg tggagcctgg gcgaccagcc gtacgccgcc gagcggcgcg
      361 ccttcgacct cgtgaacggc ctgccggagg cgctcgccta cctcctcatc cccgtgatgc
      421 aggcggggag cctggcggcg gtgttcgccg cgggcatcct cgccctgatc gcgcggcggc
      481 cgcgcctggc cggtctggtg gtgatggccg gcggctccgc gtgggtgctc gccaagctcg
      541 tcaagcgggt cgtcgagcgc gggcggcccg acgagtacct cacggacgtc gtcatccgcg
      601 gagcggtgca gatgggccag gggttcccgt ccggtcacgc cgcggtgatc gcggcgatgg
      661 ccacggtggc cacgccctat ctgagccgtc ccgggcgtct cgtcgtctgg acggtggtcg
      721 ggctggtcgc gctggcccgc gtctacacgg gcgctcacct gccactcgac gtcgtcgccg
      781 gcgtcatggt cgggtggggg ttcggctgcg tcgtcaacct cacgctgggg acaccggcat
      841 tcgcatgctg cccgcggtcc cgcctcgact gcacggtgct tgagtgacga gcggctgagc
      901 cgtgaggccc tccacggcgt agaatgccgc cgaggatgct ccggtaccac cggggaccgc
      961 ggggaggcga gagcatggcg acacggatcg tgcgctggca ccggctgcag cccccgacgc
     1021 tgcccatccg cgattggctg ccggggtacc ggcgccgctg gctgcggccc gatctcctcg
     1081 cggcggcggc ggtgtgggcg gtgctcgtgc cggaggggat cgcctacgcc tcgctcgccg
     1141 gcttgccgcc ggctaccggc ctgttcgccg ccctggcgcc gctgctggcg tacgcggtcc
     1201 tcggtacctg ccggcagttg acggtcggac cgagctcggc gatcgccgcg tactcggcgg
     1261 ccgccgtcgc gccgctcgcg ctcggcgacg acggccgctt catcgctctc tcggcgttgc
     1321 tcgccctgct cgtcggcgcg ctgctcctgg tcgcggggct ggcgcgcgcc gggttcgtcg
     1381 ccgacttctt cgcgcgcccg gtgcttaccg gcttcgtcgc cggcctcgcc ctggtcatcg
     1441 gcgtcggcca gctgtacaag ctggtgggcg tcgagagcgg cggcaccacc ttcttcggta
     1501 agctcgaggc gctggtgagc cagctcggcg acgtcaacgg cccgacgctg gccatcggcc
     1561 tgacctcgct ggccgtcatc ttcgggctcc gggcgtacgc ccccaaggcg ccgtccgcgc
     1621 tggtcgccgt cctgctcggc accgtcgcgg tcgcggtctt cgacctccag gactacggcg
     1681 tggcgatcgt cggcacgatc cccgacggct tgcctacccc cgcactgccc agcttcgggc
     1741 tcgccgacgt gatcgacctg ctgcccgacg cgttcgcgct ggcgctcatc tgcttcgcag
     1801 agtcggtggc cggcgcccgc gccctcgccg ccaagcaccg ctacgaggtg gacgccgacc
     1861 aggagctcat cgcactcggc gtctccaacc tcggggccgg cctgctgcag gggttcaccg
     1921 tcgacgccag cctgtcgcgc tcggcggtag ccgattcctc gggggtgaag tcgcagctct
     1981 ccaacatcat cctgttctgc cttctggtcg tgacgatgct gttcctcatg ccgctgttcc
     2041 acgacctgcc cgaggcggtg ctgggggcga tcgtgatcgc ggccgtcgct catctggtcg
     2101 acgtcggcgc gctgcggcgc ctgcgacggg ccgactccac cgacttcgtc gtcgccgtcc
     2161 tctgcttcgc cggggtcctg gtgttcgggc tgctcacggg gttggcgatc gcggtggtcc
     2221 tgtccctcct ggccctcgtg tatcgcgcct accgccccag ctacgccatc ctcggccggg
     2281 cgcccggcgc cgtcgacgac gagaggctgc gctaccgtgg catcgaggat caccccgacg
     2341 tgcagacgtt ccccggactc gtgatcctcc gcatcgacgg cgagctcttc ttcgccaatg
     2401 cgcgctggtt ccgcgagacc gtgcgttcgc tggtgcacga ccagagccct cccgtgcgcg
     2461 aggtgctcgt gcacgccggc gccgtgccac acatcgacac gacggccgcg gcgatgctca
     2521 aggggctgat cgccgagctg cgcgaggacg gcgtcgagct ggccctcgct cgggtgacca
     2581 ccgggctctt gcacgacctg gagcgcaacg gcgtcgcccg gctcatcggt gacgagcggt
     2641 tcttcgacac cgtcgccggg ggcgttgacg acttcctgca ccgctcgcag cctcgactcg
     2701 gctgacgcga ccccggcacg acgggggcgt ctggttggac cggccggccc ggactatgct
     2761 gtgcgccatg atcgccgaca cccccaagac ggccgccgag ctggggctca tgcgcggcag
     2821 ccccccgccc ccggacaagc gggtcacgtt ctggaactgg gagacgccgc cgttcaaccg
     2881 ctggtcgttc cagcacgtgc ccgccttcgt gcggacggcg agggtctggc gcggagacgg
     2941 gcccgtgagc cgcttcgagc ggagcccggt ccgcctcgac gacctcgcgc tcgagacccc
     3001 tgcgggcgag caggtcacgg tcgccgacgt gctgcggcgc acctgcacgg acgggttcct
     3061 cgtgctgcac gaggggcgca tcgtcgccga ggagtacttc aacggcatga cgcccgccgg
     3121 ccgccacctg ctcatgtcgg tgtccaagtc gatcgtcggc atgctcgcgg gaatcgtgat
     3181 cgggcggggc gcgctctccg ccgacgacct ggtcaccgac gtcgtgacgg agctgcgggg
     3241 tacctccttc gagcaggcga cggtgcgtca cctgctcgac atgaggacgg gcacccggtt
     3301 ctcggaggac tacgacgacc cgcagtcgga cgtgtgccgg tcggagcggg tcgccgactg
     3361 gggcccgctc ctccccggcg aggagtcctc ggggttgtac ggctacgtcc ccaccctcgg
     3421 caacgcgcgg gaccacggcg gcgccttcga gtaccgctcc atcctcacgg acgtgctcgg
     3481 ctgggtgctg gcccgggcct cgggccagag cctgcccgag cttctgagca gcgcgctctg
     3541 ggctccgctg ggcgccgagg aggacgcgga cgtgaccgtg gaccgccacg gctgcgcgtg
     3601 ggtcgacgga gggatctgcg tcacgctgcg tgatctcgcc cgtctcggcc agatgcacct
     3661 ccaggacggg tggctggacg gccggcagat cgtcccggcc gagtgggtcc gcgacacccg
     3721 cacggggggc gccgattcgc gcacggcgtt cgcggcgagc gcgtggggcg cgatgctccc
     3781 ggccgccttc taccgcaacc agttctgggt cgtccgcccc gaggacggtg tgtacaccgg
     3841 atggggcatc cacgggcagt tcctctacat cgacgtgccc gcccgcgtgg tgatcgccaa
     3901 gctctccacg ctgccgcggg cgctcgaccg ggaggtctgg gcggaccagg tggcggcgct
     3961 ggacgcgatc gcggcgtcgc tctgacggcg gtcaccacgt gatccgctcg ttcgagcgcg
     4021 gcgaggagtg gcggtactgc tacgcggacg gcgcttacgc ggagtagggt cggcggcggc
     4081 ggcgtcggct cgtgcgccgg ggtcatggtt ggccttgcgg cggcagagaa cgggcggggg
     4141 cgcctccggc ccccgcccgt gagcctcggc tccgcggccg acgtcagcgg ccctggggca
     4201 ggtagccgtc gctcagtgtc ttcatgaagg tgacgatggc cttctcctga acggcagtca
     4261 gacgcaggtt gcccatctcg gtcgtgttga cggtggccgc gtactcgggc gggtcccaga
     4321 cggcgacgtc gcgcgtgttg tagaagtgga cgatgtcccg caggctcttg aagtagccgt
     4381 tgtgcccgta ggccttcacg aagtcgggcg acggccgcag atcgacgttg cgcagtgtcg
     4441 gcaccttgtg cttccccatc tcggccgccc agacgctctc cggctcagcc atcgacttca
     4501 ggaaaccgcc gagaccgggg tcgacccagt agatgctgtt ccaggtatag acggggttct
     4561 ccaggttctt ggggatgccc aggttgtcgt aggtgaagtc ggtgaacagc ggcggcacgt
     4621 ggcagttgcc gcacttgccc ttgttcatga acagccggaa gccctgcatc tcaggcctgg
     4681 tcaacttggc cttgccggcc acccacgcgt cgtacttgga gctgaacggg ttcacctctg
     4741 acgaggcctc ataggcggcg atggacaggc cgacgtagtc gtaggcgaca tctgcgtcga
     4801 ggctgtcggg gccccacacc tgctcgaaca ggccggcata ggttgacagc ttgaccgcgg
     4861 cgatgacgtc atccgagctt gccagggcct gctcgcgcga gttcaggaac ggcccttgcg
     4921 cctgctcggc aagcgggtct cccagagtcc agccggtcgc gcgaccgtcc cagaacatgc
     4981 cgccgatcca caggccggca acctcgtcgt agtgaaggac cgggctgtca ccgccgtacg
     5041 ccgatgtcgg cggcttgcgg tcgccgaaca tcccggggtc ggagccctcg tagaccgcgc
     5101 cgtgagcatt gattgccgcg gtcggtccgg tccagccgac ttccggcgcg tggcaggtgg
     5161 cgcacgactg gtcccgattc agggacaggt tctcgtcgaa gaacagcgcc ttgcccagct
     5221 cttccaccgg tgtcagtgtg gccgcgaacg ccggcgccgc gacgcagagt agcgtcacca
     5281 gcgccgcagt cacgagcacg atcgtcttgc cgtgtctcat cggtaccccc ttggttccct
     5341 ccgtcggctg tcacgatggc gacggccgac ccatgcccgc aggcgagccg aagcccgccc
     5401 tcacgtgtcc attctgccca ccacgaaccc ggcacgaaca gtccgcttcg aggcagaagc
     5461 gctgtcagtc caggcaacgt cggcgtgtcc gtcgcgaggc cgccgacgag cgcggcgggg
     5521 ccgccgcagc acggaagacg ctgacgtcgt cgtacatgac ggcctgtggg cggaggtcct
     5581 gcctgctcgt cagggcatca gatccttcat ccgtgtcggc tcagggtacc tccgggggta
     5641 tctagctacc aacgtcggtc ggcgatccga gacagtgatc cggcgcacgt ggtccggatc
     5701 ggcacaagga gacgccacga gcgcacgcga gcaggatcgg aagcgcgatc cccgcctgga
     5761 gttcgactgc gagatcgatc cagaggccgg cgccaccgac gaggcgtgtc tgcgcgatta
     5821 tctgcgccgg aacctgccgc cggccgtgtg acgacggccg tcagacgggc agttcctcgt
     5881 ccggcggcag gtcgaccacg ccccacggtg tgccgtgcgc cgtcatcagc tcgaggaacg
     5941 gcgccgggtc gatctgctcc ggggcgaaga cgcccggccc cggggccagc cccctggcca
     6001 gcatgatcgc cgcgcaggcg gcgggcacgc cggtctgcca gccggtgccc tgcacgccgt
     6061 gcttctcgta ggcctcgtcg tgcgaggtga tctggtacat gaacctccgt acgcgcctgc
     6121 ctccgacgct gccctcggca agggtcccga cgcagacggc gccgtgcatc ctgccgatga
     6181 ggtcgaccga cctgggcagg cggctgacga ggaggtcgag cgggcggaag cgggcgccgc
     6241 cgaactcgat ctcgcggttg tggtcgaatc cgagcttgcg ccaggcgagc agcaggttgg
     6301 cccagccctc gtcgagggcg atgtagaaat cggcgttcct gaggcccttg tccccgagga
     6361 acagcggcat cagctgcgac tcctcgtggt cgacgttcca gacggtgagc ggcccgaccg
     6421 ggtcggggaa cgtgaagtcg aacttgctgg agagcgaggt gcggcggatc ttcctgccgt
     6481 cctcgtaggc gtgcggcggc aggatgaggc actcctcgac catggtcgtc ggcgagaagc
     6541 tcggcgcgaa ctcgacgccg tcggcgctgg aggcgtcgcc gtcgaggacc atgagccgct
     6601 cgacggtgtc gagctggtcg tagagcgtgc gcgcgaagac gtcgctggcg cccgggtcga
     6661 tgccgaagaa gacgagcccc gtgaggcccg ccttcttgaa cgcgtcgctg agcgccagtt
     6721 cctcatcgac gtcggcggtg ccgacgacgt cgcgcgggcc cgccgccgcc atgtcgaggt
     6781 agtgcgtgcc ggcctccaga caggcggcca tgacgtcgag gttgacctcc gggatcaaac
     6841 cgttgatcac gaggtcgacg ccggcgaggg ccttgaccag cgcctccttg tcgtggacgt
     6901 cgagctggag cagctccgcc ttgccgggcg ggagcttgcc ggcgaccgcc tgggggcgcg
     6961 acgcgtcgag gtcggcgagc acgacggcgc tcacctcggg ggcggacgcc acggtccgtg
     7021 ccgtcacctc gccgaccgaa ccgacgccca gctgcaggat gcgcatgctc atgtggggct
     7081 cccttcgaag gctggacgga gagaggcatc agcgaaccag ggccgggcta cggcgaacgc
     7141 cgatgcggcc cggaagacgc tgacgtcgtc gtacgtgcgg ccgacgatct ggatgcccat
     7201 cggcacaccg tcgcgggagc ggccgcacgg cacgacgagc accgggcagc ggctgcacag
     7261 gttgaagacc agggtcagcc cgcggcggta catcacctcg atgtcgtagt cggccgtcgt
     7321 cgccttgaga gcggggacac cgaagacggg cgtgacgagc agatcgtggt cttccaggac
     7381 accggcgatc tgcgcgtaga cctcgctctc gatctccagc gagcggtaga agccgacggt
     7441 cggcgccgcc gcgtcttcgg cgaaggcgac ggcgtacgag gtcatgaggt ggcggtggtc
     7501 gggcagcacg cgcccgatga ggctcccgaa gatcgccgcg aagtgcgccc gcgcggcctc
     7561 gcggacgtcg cccacgtcaa gccggatggc tgcgtcctcg acctgcgcgc cgaggtcgcg
     7621 gaacacgccc gcggcgacct gcaggttctc gcggacctcg tcgtccacat cgaagccgcc
     7681 gaggtccgcg tccatcgcga tgcgcaggcc ctccgccccc ggcaggtctt ccgggatcgt
     7741 gagcttcggg cgcagcgaga cgatgtcgtc gcgatgggga cccgccatca cgttctccag
     7801 caggcggcag tcctcgacgg tccgcgccat cggcccgtcg tggcagtagt ggtccaggtt
     7861 gaacggcggg agggccggga cccggccgta gggcggcttg tagccgacca ccccgcacag
     7921 cgaggcgggg atccggatgg agccgccgat gtccgagccg gtggccagca tcgcggtgcc
     7981 cgacgccagc gccgcaccgg acccgcc
//
LOCUS       DASUMX010000202         4002 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_214067, whole genome shotgun sequence.
ACCESSION   DASUMX010000202 DASUMX010000000
VERSION     DASUMX010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4002
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214067"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="VFZ86_09910"
     CDS             <1..352
                     /locus_tag="VFZ86_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642636.1"
                     /translation="LRPAIVRATIAVRHFVHVAARPKIVRRGARVKISGTVAPTARQL
                     GADASAVTLRVERSTASGWRKVAGRTVTPRADGAYSWSWRPRKAGSYRVRATAAATQE
                     LLAGASAPVRIRVR"
     gene            560..1084
                     /gene="infC"
                     /locus_tag="VFZ86_09915"
     CDS             560..1084
                     /gene="infC"
                     /locus_tag="VFZ86_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013132287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEX5642637.1"
                     /translation="MRDRANEPRIRINDGITVPQVRLIDENGEQLGIKPIRFAQDYAA
                     DKNLDLVEVAPTADPPVCRIMNYSKYKYELELKAKQARKHQSQIVIKEIKFRPKVGVH
                     DYETKKGHVLRFLEHKDKVKVTIMFRGREMVHPERGEALLMRLAEDVKEYGVIESKPL
                     QDGRNMVMMLAPVK"
     gene            1101..1298
                     /gene="rpmI"
                     /locus_tag="VFZ86_09920"
     CDS             1101..1298
                     /gene="rpmI"
                     /locus_tag="VFZ86_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017614403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEX5642638.1"
                     /translation="MPKTKTHSASKKRFRLTGTGKVVRRHGMKSHLLEHKSSKRKRGF
                     RKLYDVSPADSKGIKRLLGLR"
     gene            1375..1746
                     /gene="rplT"
                     /locus_tag="VFZ86_09925"
     CDS             1375..1746
                     /gene="rplT"
                     /locus_tag="VFZ86_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016700250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEX5642639.1"
                     /translation="MSRVKRSVSARKKRRKVLEQAKGYRATKNSSYKRAKEQVQRSGQ
                     YAYRDRRVRKREFRKLWIVRINAGARENGLSYNQFMHGLQLAEIELDRKILADLAVSD
                     PAYFASLVDQARAAIDAAKAA"
     gene            1884..2618
                     /locus_tag="VFZ86_09930"
     CDS             1884..2618
                     /locus_tag="VFZ86_09930"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX5642640.1"
                     /translation="MVAAALAAGYRPQAVFLREEAAGELGERLGLDPASVFTVVDRVA
                     ARISTLETPADVVAVLPLPERRPVSSLLGPAVVPLVVYVDGVQDPGNVGTLIRAAVAF
                     GASALVTSAGSADLYGPKTVRASMGALFGLPVFAELPLGDVVGQLPAATVYGLAAHGG
                     EPLDGARLERPAVLVVGAERAGLSRAALAHVGRLLTIPLPAAPGGAVESLNAGVAGAI
                     ALYEFSRRPAGAPEDPSRRSPAAQEG"
     gene            2621..3712
                     /gene="pheS"
                     /locus_tag="VFZ86_09935"
     CDS             2621..3712
                     /gene="pheS"
                     /locus_tag="VFZ86_09935"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEX5642641.1"
                     /translation="MDDAHLQELKQRAPGLAAIYHTGRDTVLRAADLAALEQARVQIL
                     GRKSALTEFLRSIPTLPAEERPLVGKGGNLIRKELEELVEERETELKREALSDSLAHE
                     RIDVTLPGQPFPAGHEHLITQTMREVEDIFIGLGYSIAEGPEVELDYYNFTALNTPPS
                     HPARSAHDTFWVVEQRETMPEAVVDADRGVLLRTHTSPVQVRVMERQQPPVYVVCLGK
                     VYRRDSDATHTPMFHQVEGLVVDEGITLADLKGTVAHFTRAFFGPDRSIRVRPHFFPF
                     TEPSIEVDVDCQLCGGKGCRSCKYSGWLEIMGAGMVDPAVYSFVGYDPEKVSGFAFGM
                     GVERMAMLRHGIPDLRLFYDNDVRFLRQF"
     gene            3729..>4002
                     /locus_tag="VFZ86_09940"
     CDS             3729..>4002
                     /locus_tag="VFZ86_09940"
                     /inference="COORDINATES: protein motif:HMM:NF013734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX5642642.1"
                     /translation="MRVPFSWIKEYVAWDGTVEEMAEVLTMSGTEVEGIDWVGAPRDP
                     ENLGRFVVGKVLTREKHPNADKLNLCTVEVGERNGGVRQIVCGAHNF"
BASE COUNT          653 a         1426 c         1366 g          557 t
ORIGIN      
        1 gctgcgaccc gccatcgtcc gcgcgacgat cgccgtcagg cacttcgtgc acgtggcggc
       61 gcggccgaag atcgtgcgcc gcggcgcgag ggtcaagatc tcgggcacgg tcgcgccgac
      121 ggcgcggcag ctcggcgccg acgcctccgc cgtgacgctg cgcgtcgagc gcagcacggc
      181 gtccgggtgg cgcaaggtcg ccgggcgcac cgtcacgccg cgcgccgacg gcgcgtattc
      241 gtggtcctgg cggccgcgca aggccgggtc gtaccgtgtc cgcgcgaccg ccgcggcgac
      301 gcaggaactg ctcgccggcg ccagcgcgcc ggtccggata cgggtccgct gaccgggaac
      361 ggcgcgctgc gggctgttcg acgctcgcag cccgttcgtg tatactgccg tgcgccacaa
      421 gtgggagctt gcgagcaccc caccctgcgc ccgttggtag gccgacaggg tccgtcacga
      481 gaaaggagat cgtgagggct cgccgctggc gagccttttt ccgtatatgg gcagaccatt
      541 ttctccgaga gatagattca tgagggaccg ggccaacgag ccccgtatcc gcatcaacga
      601 cggcatcacg gtgccgcagg tgcgcctcat cgatgagaac ggcgaacagc tgggcatcaa
      661 gcccatccgc ttcgcccagg actacgcagc cgacaagaac ctcgacctcg tcgaggtagc
      721 gccgaccgcc gacccgcctg tgtgccgcat catgaactac agcaagtaca agtacgagct
      781 ggagctcaag gccaagcagg cgcgcaagca ccagagccag atcgtgatca aggagatcaa
      841 gttccggccc aaggtcggcg tgcacgacta cgagaccaag aagggtcacg tgctccgctt
      901 cctggagcac aaggacaagg tcaaggtcac gatcatgttc cggggacgcg agatggtcca
      961 tccggagcgc ggcgaggcgc tgctcatgcg cctcgcggag gatgtcaagg aatacggcgt
     1021 catcgagtcc aagcccctgc aagacgggcg caacatggtc atgatgctcg cgccggtgaa
     1081 gtagctggag ggaggtacgc atgccgaaga ccaagactca cagcgcgtcc aagaagcgct
     1141 ttcgcctcac cggcacgggc aaggtcgtcc gtcgccacgg gatgaagagc cacctcctgg
     1201 agcacaagag ctcgaaacgt aagcggggct tccgcaagct gtacgacgtc agcccggctg
     1261 attccaaggg gatcaagcgg ctgctcgggc tccgctgagc ctgccgacct aagatcacga
     1321 gccggcgcgg ccgcttgatg gccacgaacc ggtgcagttc caaggagtac gacaatgtct
     1381 cgtgtcaaac gaagcgtcag tgcgcgcaag aagcgccgca aggtactcga gcaggccaag
     1441 ggctaccgcg cgaccaagaa ctcgtcctac aagagggcga aggagcaggt ccagcggagc
     1501 ggtcagtacg cgtaccgcga ccgccgcgta cgcaagcgcg agttccgcaa gctctggatc
     1561 gtgcgcatca acgcgggcgc gcgggagaac ggtctctcgt acaaccagtt catgcacggg
     1621 ctgcagctcg ccgagatcga gctcgaccgc aagatcctcg ccgacctcgc ggtgagcgac
     1681 ccggcctact tcgcctcgct cgtcgaccag gcccgcgcgg ccatcgacgc ggccaaggcc
     1741 gcctgacgac cgggagcgag tcgcttgggg ttttcgggcc accactgacc agcccgcaga
     1801 acgccctcgt caagcgggcg gcgtctctgc gccagcgcaa gttccgcgac cgcgaaggcg
     1861 ccttcctggc ggagggcgcc gacgtggtcg cggctgcgtt ggcggcgggg taccgcccgc
     1921 aggccgtgtt cctgcgcgag gaggccgcgg gcgagctcgg cgagcgcctc ggcctcgatc
     1981 cggcgtccgt cttcaccgtc gtcgatcgcg tggcggcgcg catctccacg ctggagacgc
     2041 ccgccgacgt ggtcgccgtg ctgccgctgc cggagcgccg gcccgtctcg tcgctgctcg
     2101 ggccggccgt cgtccccctc gtggtctacg tcgacggcgt ccaggacccg ggcaacgtcg
     2161 gcaccctgat ccgggcggcg gtcgccttcg gcgcctccgc cctcgtcacg tcggcgggct
     2221 cggccgacct ctacggcccc aagacggtcc gcgccagcat gggcgcgctc ttcggcctgc
     2281 cggtgttcgc cgagctgccc ctcggcgacg tggtggggca gctgccggcg gcgaccgtgt
     2341 acgggctcgc cgcgcacggc ggcgagccgc tggacggcgc tcgactcgaa cggcccgccg
     2401 tgctcgtggt cggcgccgag cgggccggcc tctcgcgcgc cgcgctcgcg cacgtcggcc
     2461 gcctgctcac gatcccgctg ccggcggcgc cgggcggcgc cgtcgagagc ctcaacgccg
     2521 gcgtggccgg cgcgatcgcc ctctacgagt tctcgcgccg cccggccggc gctcccgaag
     2581 acccgtcacg ccgctctccc gcggcgcagg aaggatgacc atggacgacg cccacctgca
     2641 ggagctgaag cagcgagccc caggcctcgc ggccatctac cacaccggcc gcgacaccgt
     2701 cctgcgcgcc gccgacctgg ccgcgctcga gcaggcgcgc gtccagatcc ttgggcgcaa
     2761 gagcgcgctc acggagttcc tgcgctcgat ccccaccctg cccgccgagg agcgcccgct
     2821 ggtcggcaag ggcggcaacc tcatccgcaa ggagctcgag gagctcgtgg aggagcgcga
     2881 gacggagctg aagcgcgagg cgctctccga ctcgctggcc cacgagcgca tcgacgtcac
     2941 cctgcccggc cagccgttcc cggccggtca cgagcacctc atcacccaga cgatgcgcga
     3001 ggtcgaggac atcttcatcg gcctcggcta cagcatcgcc gagggtcccg aggtcgagct
     3061 cgactactac aacttcacgg cgctcaacac cccgcccagc caccccgcgc gctccgccca
     3121 cgacacgttc tgggtcgtcg agcagcgcga gaccatgccc gaggcggtcg tggacgccga
     3181 ccgcggcgtc ctgctgcgca cccacacctc tcccgtccag gtgcgcgtca tggagcggca
     3241 gcagccgccc gtgtacgtcg tctgcctcgg caaggtctac cggcgcgact cggacgcgac
     3301 gcacacgccg atgttccacc aggtcgaggg cctcgtggtc gacgagggca tcaccctggc
     3361 cgacctcaag ggcaccgtcg cgcacttcac gcgggcgttc ttcgggccgg accgcagcat
     3421 cagggtccgg ccgcacttct tcccgttcac cgagccgtcc atcgaggtcg acgtggactg
     3481 ccagctctgc ggcggaaagg gctgccgctc gtgcaagtac tccggctggc tcgagatcat
     3541 gggcgccggc atggtcgacc cggcggtgta cagcttcgtc ggctacgacc cggagaaggt
     3601 cagcggcttc gccttcggta tgggggtgga gcgcatggcg atgctgcggc acggcatccc
     3661 cgacctgcgc ctgttctacg acaacgacgt ccgcttcctg cggcagttct gaaggagggc
     3721 ggatcacgat gagagtcccg ttcagctgga tcaaggagta cgtcgcctgg gacggcacgg
     3781 tcgaggagat ggccgaggtc ctcaccatgt ccggcaccga ggtggagggc atcgactggg
     3841 tcggcgcgcc gcgcgacccg gagaacctcg gccgcttcgt cgtcggcaag gtgctcacca
     3901 gggagaagca ccccaacgcc gacaagctca acctctgcac cgtcgaggtg ggggagagga
     3961 acggcggcgt gcggcagatc gtctgcggcg cccacaactt cg
//
LOCUS       DASUMX010000203         5112 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1365784, whole genome shotgun sequence.
ACCESSION   DASUMX010000203 DASUMX010000000
VERSION     DASUMX010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 5112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5112
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1365784"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1460)
                     /locus_tag="VFZ86_09945"
     CDS             complement(<1..1460)
                     /locus_tag="VFZ86_09945"
                     /inference="COORDINATES: protein motif:HMM:NF023650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HEX5642643.1"
                     /translation="MDALSPGAGQPVVVARRGPLRRFGAWYRRHVWAATALLILLVAV
                     AGFAALQVAAWGKVGMGVAVAGQRLEGLDRREAAAMVAFRLVDRPGEVRLDLGEAEPL
                     TLSFRQLGITVDPEATAQRALEAGRTRLPLGLSLWLPGRAQDVAPVIRVDATRYKKGL
                     EAVRAQVDAPARDARLKLSGGGVAVVAAEDGREVDAVALERALLAALGAGRRYAGPVP
                     TRVIRPEVSTVDAEARASAAAAYLARPVTLRYRSEAVTLTPAQMAGMLSVNTGGDADQ
                     YPLTFHNKRAAEELHRIFAFAETPPVDATVEVNDDGTVNVTESRSGMLLDMPVLMDEL
                     DDAATGGGLRTIFVALTPAFPELSTADVENMGLSSLGSQFVTYFDTRNTSRATNIALA
                     AKLVDGAVVEPGETFSLNAAMGPRTANRGFDYAPVIAADGVLRQGVGGGICQYATTLF
                     NAVFFAGLPVVEREAHSLYISHYPIGRDATVAWGAV"
     gene            complement(1505..4033)
                     /locus_tag="VFZ86_09950"
     CDS             complement(1505..4033)
                     /locus_tag="VFZ86_09950"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX5642644.1"
                     /translation="MRAVVMAGGEGTRLRPLTSNQPKPMVSLCGKPCMEHIVELLKRH
                     GIEETVVTLMFLPKVIRDYFGDGSTLGVRMQYSVEQAPAGTAGSVKLAEDELRDDTCI
                     VISGDALTDFDLSEIVAFHKQRGAMVTIALKRVENPLEFGVVVVDEEGRIERFLEKPT
                     WGQVFSDTVNTGIYVLEPEVFDHIPADTKYDFSQELFPKLFEMGAPLYGYVAEGYWQD
                     IGSLPQYLTANRDLLDGKVQAGLPGIELQNRIFLGDGVNLDSLENMQGPAFVGNYVKI
                     HPTASIGAYSVLGSNVVVKEHAETRNSVIAANTYVGASVKVFGSIIGRNCDLKAGATF
                     SEGVAVGDESVVGEQAYIGPDVKIYPNKTVESGAQINSSIIWESRGSSQLFGKDGITG
                     LINVDITAELALKMAMAYGTVLKKGARVTTSRDAHPASRVIKRAIISGLNSTGVVVRD
                     LRIASSAINRFEVKNGNTQGGVHVRIASWDPEMIQIQIIEPPGINLSEARQKDIEKYY
                     GRQDFRRAFYSEFGEIEFPDRAMETYVRGLLRSWDVERIHSRAYRLVIDYSYSPASLT
                     LPYLLGSLGAEVLSVRAFNEQHHVSMGAEELGANIQEVQRLVRTMEADLGLVIGPGAE
                     RLYVVDDRGAEVPLEKALLLFVKLVAQQASRGEKLVLPLTVTHLAEALAEPFGVEVER
                     TKVSLPALAQASTEDGVVFAGSLGGGYIFPRFLPAFDAVMSLGKLLELLAPQARPLSE
                     QLTEIPTSTLVHKTVACPWALKGTVMRTATEHLQREAADGGARIGLLDGIQLRRDGGW
                     VQLLPDADEPVFHVYAEGADHEESERLAEGFLEVVRGVIRDNAG"
     gene            complement(4105..4686)
                     /locus_tag="VFZ86_09955"
     CDS             complement(4105..4686)
                     /locus_tag="VFZ86_09955"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX5642645.1"
                     /translation="MEHAPATGLVRYVPNALTVLRLCALPVIVYLYSLDVPGSSWTTA
                     IVILFAALSDVADGLIARKYHVQSEFGRWVDPIVDRAFFFTIVAMLWYFGTLPWLAVV
                     PLLIRDGIILVLAIPTRVYTTKGPEISRWGKASNFILICALQWFIVDLRVLGWAFFAV
                     GATLYIASGLWYGYKVILYVRRAGPGDGEAAPN"
     gene            4777..4854
                     /locus_tag="VFZ86_09960"
     tRNA            4777..4854
                     /locus_tag="VFZ86_09960"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4812..4814,aa:Pro,seq:ggg)
BASE COUNT          739 a         1747 c         1840 g          786 t
ORIGIN      
        1 tccacggcgc cccacgccac ggtggcgtcg cggccgatcg ggtagtgcga gatgtagagc
       61 gagtgcgcct cgcgctccac caccggcagg ccggcgaaga acacggcgtt gaagagcgtg
      121 gtggcgtact ggcagatgcc gccgccgacg ccctggcgca gcacgccgtc ggcggcgatc
      181 accggcgcgt agtcgaagcc gcggttggcc gtgcgcgggc ccatggccgc gttcagggag
      241 aacgtctccc ccggctcgac cacggcgccg tcgacgagct tcgccgccag cgcgatgttg
      301 gtggcgcgcg aggtgttgcg cgtgtcgaag taggtgacga actgggagcc gagcgacgag
      361 agccccatgt tctccacgtc cgccgtgctg agctcgggga acgcgggcgt gagcgccacg
      421 aagatggtgc gcaggccgcc cccggtggcc gcgtcgtcga gctcgtccat gagcacgggc
      481 atgtcgagca gcatgcccga cctgctttcg gtgacgttga cggtgccgtc gtcgttgacc
      541 tcgaccgtgg cgtccaccgg cggcgtctcg gcgaaggcga agatgcggtg cagctcctcg
      601 gcggcacgct tgttgtggaa ggtgagcggg tactggtcgg cgtcgccgcc ggtgttgacg
      661 gagagcatgc cggccatctg cgccggcgtg agcgtcacgg cctcggagcg gtagcggagc
      721 gtgaccgggc gcgcgaggta ggcggccgcg gcggaggcgc gggcctccgc gtcgacggtg
      781 ctcacctcgg gccggatgac cctggtcggg accggaccgg cgtagcggcg cccggcgccc
      841 agcgcggcca gcagggcgcg ctcgagagcg accgcgtcga cctcgcgccc gtcctcggcc
      901 gcgacgacgg cgacgccccc gccgctgagc ttgagccgcg cgtcgcgggc cggcgcgtcg
      961 acctgcgcgc gcacggcctc gaggcccttc ttgtagcggg tcgcgtcgac gcggatgacg
     1021 ggggcgacgt cctgcgcgcg gccgggcagc cacaggctga gaccgagcgg cagccgcgtc
     1081 ctgccggcct ccagcgcccg ctgcgcggtc gcctcggggt cgacggtgat gccgagctgc
     1141 cggaacgaca gcgtgagcgg ctcggcctcc cccaggtcga ggcggacctc cccgggccgg
     1201 tcgacgagcc ggaaggcgac catggcggcc gcctctctgc ggtccagccc ctcgagccgc
     1261 tgcccggcga ccgcgacgcc catgccgacc ttgccccagg cggcgacctg cagcgcggcg
     1321 aagccggcga ccgcgaccag gaggatcagc agcgcggtcg ccgcccacac gtgacggcga
     1381 taccacgcgc cgaagcgccg cagcggtccg cggcgcgcga cgacgacggg ctgcccggcc
     1441 ccgggcgaga gtgcgtccat accatccatt gtaccggcag ccttcgccgc cggcctcccg
     1501 cgtcctaccc ggcgttgtcc cggatgacgc cgcggacgac ctcgaggaag ccctcggcca
     1561 ggcgttcgga ctcctcgtgg tcggcgccct cggcgtacac gtggaagacg ggctcgtcgg
     1621 cgtcgggcag cagctgtacc cagccgccgt cgcggcgcag ctggatgccg tcgaggaggc
     1681 cgatcctggc gccgccgtcc gcggcctcgc gctgcaggtg ctcggtggcg gtgcgcatga
     1741 ccgtgccctt gagcgcccac gggcacgcca ccgtcttgtg gacgagcgtg ctggtgggga
     1801 tctcggtgag ctgctcgctc agcggtcgcg cctgcggcgc gagcagctcg agcagcttgc
     1861 ccaggctcat gacggcgtcg aacgccggca ggaagcgcgg gaagatgtag ccgccgccga
     1921 gcgagccggc gaacaccacg ccgtcttcgg tggacgcctg ggcgagcgcc ggcagcgaca
     1981 ccttggtgcg ctccacctcg acgccgaagg gctcggccag cgcctcggcg aggtgcgtca
     2041 ccgtgagcgg cagcacgagc ttctcgccgc gggaggcctg ctgcgcgacg agcttgacga
     2101 acagcagcag cgccttctcc agcggcacct cggcgcccct gtcgtcgacc acgtacaggc
     2161 gctcagcgcc ggggccgatg accagcccga ggtcggcctc catggtgcgc acgagacgct
     2221 ggacctcctg gatgttggcg ccgagctcct cggcgcccat gctcacgtgg tgctgctcgt
     2281 tgaaggcacg caccgagagc acctcggcgc cgaggctgcc cagcaggtac ggcagcgtca
     2341 gcgacgccgg cgagtacgag tagtcgatga ccagccggta ggcgcggctg tggatgcgct
     2401 ccacgtccca ggagcggagg aggccacgca cgtaggtctc catggccctg tccgggaact
     2461 cgatctcgcc gaactcgctg tagaaggcgc ggcggaagtc ctggcggccg tagtacttct
     2521 cgatgtcctt ctggcgcgcc tcgctcaggt tgatgcccgg aggctcgatg atctggatct
     2581 ggatcatctc cgggtcccag ctcgcgatgc gcacgtggac gccgccctgc gtgttgccgt
     2641 tcttgacctc gaagcggttg atggcgctgc tggcgatgcg caggtcgcgg accacgacgc
     2701 cggtggagtt gagccccgag atgatggcgc gcttgatgac ccgcgacgcc gggtgggcgt
     2761 cgcggctcgt ggtgacgcgc gcgcccttct tgagcaccgt gccgtaggcc atcgccatct
     2821 tcagcgcgag ctcggcggtg atgtcgacgt tgatgagccc ggtgatgccg tccttgccga
     2881 agagctgcga gctgccgcgc gactcccaga tgatgctgct gttgatctgg gcgccggact
     2941 cgaccgtctt gttggggtag atcttgacgt ccgggccgat gtaggcctgc tcgccgacca
     3001 ccgactcgtc gccgaccgcc acgccctcgc tgaacgtggc cccggccttg aggtcgcagt
     3061 tgcgcccgat gatggacccg aagaccttga ccgaggcgcc cacgtaggtg ttcgcggcga
     3121 tgacgctgtt gcgcgtctcg gcgtgctcct tgaccacgac gttgctgccc aggaccgagt
     3181 aggccccgat ggaggccgtg gggtggatct tgacgtagtt gcccacaaag gcggggccct
     3241 gcatgttctc gagcgagtcc agattgacgc cgtcgccgag gaagatgcgg ttctgcagct
     3301 cgatcccggg cagcccggcc tggaccttgc cgtccagcag gtcgcggttg gcggtgaggt
     3361 actgcggcag gctgccgatg tcctgccagt agccctcggc gacgtagccg tagagcggcg
     3421 cccccatctc gaacagcttg gggaacagct cctggctgaa gtcgtacttg gtgtccgcgg
     3481 ggatgtggtc gaacacctcg ggctcgagca cgtagatgcc ggtgttgacg gtgtcgctga
     3541 agacctggcc ccaggtgggc ttctcgagaa agcgctcgat ccgcccctcc tcgtcgacca
     3601 ccacgacgcc gaactcgagc gggttctcca cgcgcttgag ggcgatggtg accatggcgc
     3661 cgcgctgctt gtgaaaggcg acgatctcgc tgaggtcgaa gtcggtcagc gcgtcgccgc
     3721 tgatgacgat gcacgtgtcg tcgcggagct cgtcctcggc cagcttgacg ctgccggcgg
     3781 tgccggccgg agcctgttcc accgagtact gcatgcgcac gccgagcgtg gacccgtcgc
     3841 cgaagtagtc gcggatgacc ttgggcagga acatgagcgt gacgacggtc tcttcgatgc
     3901 cgtgccgctt gagcagctcc acgatgtgct ccatgcacgg cttgccgcac agcgacacca
     3961 tcggcttggg ctggttgctc gtgagcggcc gcaggcgggt gccctcgccg ccggccatga
     4021 ccactgcgcg cacgcgtcac ctcagctgtt cggaggaccg accccagtac ggctagcgta
     4081 ccagatgcgc gcgggcgtca ggcgtcagtt cggggcggct tcgccgtcgc ccggaccggc
     4141 ccggcgtacg tacaggatga ccttgtagcc gtaccagagc ccgctggcga tgtagagcgt
     4201 cgccccgacg gcgaagaacg cccagccgag caccctcagg tcgacgatga accactgcag
     4261 cgcgcagatg aggatgaagt tggacgcctt gccccagcgg ctgatctccg ggcccttggt
     4321 cgtgtagacg cgcgtgggga tcgccaggac caggatgatg ccgtcgcgga tgagcagcgg
     4381 caccaccgcc agccacggca gcgtcccgaa gtaccacagc atggcgacga tcgtgaagaa
     4441 gaaggcgcgg tcgacgatgg ggtccaccca gcgcccgaac tcgctctgca cgtggtactt
     4501 gcgcgcgatg agcccgtcgg cgacgtcgct gagcgcggcg aagaggatga cgatcgccgt
     4561 cgtccagctg gagcccggca catccaggct gtacaggtag acgatcaccg gcagcgcgca
     4621 cagccgcagc accgtgagcg cgttggggac gtagcggacg aggccggtgg ccggggcgtg
     4681 ttccataggc gggagcatag caggcccggc cgcggcggct ctgccccgcc gcccggccgg
     4741 cccgtcgtgc ctgtgctaaa atgcccgctg tacggtcggc gtgtggcgca gcctggttag
     4801 cgcactagtc tgggggacta ggggtcgcca gttcaaatct ggccacgccg accatcttct
     4861 tcctcctccg cgcacagcac tcgggccgcg cgcgcgggca tcggtcagac cctgatcctc
     4921 ttcgtggcgt atcgcagaag cagtggcagc gcggccatga ctgcccacac gagtagtcgg
     4981 cgtttcatgc ttggcctctc atcggcgtgc gcaaggccct gcgtctactc ccgcagggcc
     5041 ttggcgaact ccacgacctc actgacgttc tggacgggtg tgatgtcgaa gtcctgcgcg
     5101 atcgctatcg ag
//
LOCUS       DASUMX010000204         2062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1797717, whole genome shotgun sequence.
ACCESSION   DASUMX010000204 DASUMX010000000
VERSION     DASUMX010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1797717"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1854)
                     /locus_tag="VFZ86_09965"
     CDS             complement(<1..1854)
                     /locus_tag="VFZ86_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642646.1"
                     /translation="MIVAAAGPPTYLVISAGALIVGVIAIAAFRYWPRRKPPAPEAAT
                     AETEIDEPPADRHVRTPSDALHLIVGVLLVLLGMLIAAGASNTLVGFERDTIGAFDAL
                     PEALSRALYLLFQAVASLIPAAVGLVVLWRRRFRLFGMLILAALVAQSLASFLNSVIV
                     ETFGTPELLVAVHRPEWLRDVASIGVIDSSFVAGMVSTVVLGSPWISRAWRRLGWSVV
                     VVAVLFRIVAAQEGPLDVVLAVGAGVLGGSLVLLVFGAPNKRPRGPAVVAALVRAGIP
                     LVRLERAGVDARSSTPYFGETEDGSSIFIKVLGRDERSADLMFRIYRFLRLKDVGDKG
                     PFTSLQRMVEHEALVALYANDSGVLTPRLVVTTSVGDDGFLLAYERTAGDSLDRVEDE
                     ALSDDVLRGIWEGVAVMRQRRIAHRDLRLANVMLAPDGRPWFIDFGFAEIAATDQMLD
                     TDAAELLSSTALKVGPERAVDAAVAVLGKPAVASAAPRIQPLALSTATRKSLVARKPL
                     CDDLRKYAAEASGAGDVEPQDLQRIKPRTVLTFIALGLAFYVLIPQLADVSGVLTELR
                     HADWTWALWAVVASAATYVAAAIGLMGAVPGHVPFFGTVGAQLAGSFVNRIT"
BASE COUNT          319 a          727 c          768 g          248 t
ORIGIN      
        1 cgtgatgcgg ttcacgaacg agcccgcgag ctgggcgccg accgtgccga agaagggcac
       61 gtgccccggc acggcgccca tgagcccgat cgccgcggcg acgtacgtgg cggccgacgc
      121 gaccactgcc cacagcgccc acgtccagtc ggcgtgcctg agctccgtca gcacgccgct
      181 gacgtcggcc agctgcggga tgaggacgta gaacgccagt ccgagcgcga tgaacgtgag
      241 gaccgtgcgc ggcttgatac gctgcaggtc ctgcggctcg acgtcccccg cgccgctcgc
      301 ctcggctgcg tacttgcgca ggtcgtcgca gagcggcttg cgcgccacca gcgacttgcg
      361 cgtggccgtg ctgagcgcga gcggctggat gcgcggcgcc gcgctcgcca ccgccggctt
      421 gccgagcacg gcgacggcgg cgtcgaccgc gcgttcgggc cccaccttga gggcggtcga
      481 cgagagcagc tccgccgcat cggtgtcgag catctggtcg gtggccgcga tctcggcgaa
      541 gccgaagtcg atgaaccagg ggcggccgtc cggcgccagc atcacattgg cgagccgcag
      601 gtcgcgatgc gcgatgcggc gctgccgcat caccgcgacg ccttcccaga tgccgcgcag
      661 cacgtcgtcg ctgagcgcct cgtcctcgac gcggtcgagc gagtcgccgg cggtgcgctc
      721 gtaggccagc agaaagccgt cgtcgccgac gctggtggtc acgacgaggc gcggcgtgag
      781 caccccggag tcgttcgcgt agagcgcgac gagcgcttcg tgctccacca tgcgctgcag
      841 cgaggtgaac ggccccttgt cgccgacgtc cttcaggcgc aggaagcggt agatgcggaa
      901 catcaggtcg gcgctgcgct cgtcgcggcc cagcaccttg atgaagatcg agctgccgtc
      961 ctcggtctcg ccgaagtagg gggtggagct gcgcgcgtcc acgccggctc gctcgagacg
     1021 gaccagcggg atgccggccc ggaccagggc ggcgacgacc gccgggcccc ggggacgctt
     1081 gttgggcgcc ccgaagacga gcagcaccag ggacccgccg agcacgccgg cgcccaccgc
     1141 cagcacgacg tccagcggac cctcctgcgc cgcgacgatg cggaagagca ccgccacgac
     1201 gacgacggac cagcccaggc gccgccaggc ccggctgatc cacggcgacc ccagcacgac
     1261 cgtggagacc atgccggcga cgaagctgga gtcgatcacg ccgatggagg cgacgtcgcg
     1321 cagccactcc ggccggtgga cggcgaccag cagttccggc gtcccgaagg tctcgacgat
     1381 gacgctgttc aggaacgacg ccagcgactg cgcgacgagg gccgcgagga tcagcatccc
     1441 gaagagccgg aacctgcggc gccacagtac gaccagcccg acggcggcgg ggatgagcga
     1501 ggccacggcc tggaacagca ggtagagcgc ccgcgagagc gcctcgggca gggcgtcgaa
     1561 ggcgccgatc gtgtcccgct cgaacccgac cagcgtgttc gaggcgccgg ccgcgatcag
     1621 catgccgagg agcacgagga gcacgccgac gatgaggtgg agcgcgtccg acggcgtgcg
     1681 cacgtggcgg tcggccggcg gctcgtcgat ctcggtctcg gcagtcgccg cctcgggggc
     1741 cggcggcttg cgcctcggcc agtaccggaa cgcggcgatg gcgatgacgc ccacgatgag
     1801 agcgccggcc gagatcacca ggtacgtcgg ggggccggcg gcggcgacga tcacgactca
     1861 ggcccgcttg ccgcgccggt cggcgcgcgg ccgcgtcgag agagaggggg tcgagccgat
     1921 cgtcctgtct cggtcgcgcg gtctcttcat ggtggtgcgc gcgccttgag ggacggcgcg
     1981 gattcgaggg tgtgcggtcc tgacagcacc atccccggga tccgggagga caaccacgac
     2041 cgcgtttact cccgtggccg gc
//
LOCUS       DASUMX010000205         2033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_360346, whole genome shotgun sequence.
ACCESSION   DASUMX010000205 DASUMX010000000
VERSION     DASUMX010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360346"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VFZ86_09970"
     CDS             <1..190
                     /locus_tag="VFZ86_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642647.1"
                     /translation="LLPVSAQPAAGVVAPTAAMVGRAVACGAPGVVRGPDAVLPLYGR
                     APDAKPRPGAASVAGGTS"
     gene            187..675
                     /gene="rimI"
                     /locus_tag="VFZ86_09975"
     CDS             187..675
                     /gene="rimI"
                     /locus_tag="VFZ86_09975"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEX5642648.1"
                     /translation="MNLRVVPDRPHHELVVRPMQFPDLEAVAALEARTFTLPWSVAVF
                     SGQLARESGICLVCEDGGRIIAYLIADMFVDVWHLMNLCVAEEARRGHVASLLLEAYF
                     AITERKGHRGHTLEVRVSNGPAIELYRSFGFVSTGVRPGYYSDDREDAVIMWKDWEGD
                     SA"
     gene            672..1751
                     /gene="tsaD"
                     /locus_tag="VFZ86_09980"
     CDS             672..1751
                     /gene="tsaD"
                     /locus_tag="VFZ86_09980"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEX5642649.1"
                     /translation="MTEAAHAGQAPPTRLILAVETSCDDTSAAVVRGREVLSSIVSSQ
                     NELHAQFGGVVPEVASRRHTELVNEVVAEAMSQAGAGWDDLGAVAVTQGPGLIGALLV
                     GLATGKSLAYRRGLPLIPVNHLQGHIAANYALGVEAPFVCLVASGGHTLLAVVEEGVR
                     YRVAAKTMDDAAGEAFDKGARLLGLGYPGGKELDGLAARGDPGFARFPRAVPRGRGFS
                     FSGLKTALLYDLRGREEAEIAAHRADIAASYQAAIVRQLVDKTLAGAAAEGLRTVAVA
                     GGVAANSGLRRALTEACEAEGLRLSLPPLSLCTDNAGMIGLAAGFLPAIPWPGYLDLD
                     AFASDAEAKAARPPRKGSARRAPRP"
     gene            1852..>2033
                     /locus_tag="VFZ86_09985"
     CDS             1852..>2033
                     /locus_tag="VFZ86_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX5642650.1"
                     /translation="MTHRPASVFPPAPLSDAPRPAANLFVDCLENEGVEFVFGIPGEE
                     TLDLSEALDRSQLITFV"
BASE COUNT          254 a          761 c          728 g          290 t
ORIGIN      
        1 gctgctgccc gtgtcggcgc agccggccgc gggcgtcgtc gcgccgacgg ccgcgatggt
       61 gggccgggca gtcgcctgcg gcgcgcccgg cgtcgtgcgc ggccccgacg cggtgttgcc
      121 cctctacggc cgcgctcccg acgccaagcc gcgtccgggc gcggcgtcgg tcgccggagg
      181 gacgtcatga acctcagggt cgtccccgac aggccgcatc acgagcttgt ggtgcggccg
      241 atgcagttcc ccgacctgga ggcggtggcg gccctggagg cgcgcacgtt cacgctgccg
      301 tggtcggtcg ccgtcttcag cggtcagctc gcgcgcgaga gcggcatctg tctcgtctgc
      361 gaagacggcg gccgcatcat cgcctatctc atcgccgaca tgttcgtcga cgtatggcac
      421 ctcatgaacc tctgcgtcgc cgaggaggcg cggcgcgggc acgtcgcctc gctcctcctg
      481 gaggcgtact tcgccatcac cgagcgcaag gggcaccgcg ggcacacgct ggaggtccgc
      541 gtctccaacg gccccgccat cgagctgtac cgcagcttcg ggttcgtctc cacgggggtc
      601 cgccccgggt actacagcga cgaccgcgag gacgcggtca tcatgtggaa ggactgggaa
      661 ggtgactcgg cgtgacggag gctgcccacg ccgggcaggc tccgcccacc cgcctcatcc
      721 tcgccgttga gacctcgtgc gacgacacca gcgcggcggt cgtgcgcggg agggaggtcc
      781 tctcgtcgat cgtctcgtcc cagaacgagc tgcacgcgca gttcggcggc gtggtgccgg
      841 aggtggcctc gcgccgccac accgagctcg tcaacgaggt cgtcgccgag gcgatgagcc
      901 aggccggcgc cggctgggac gacctcggcg ccgtggccgt gacgcagggc ccggggctca
      961 tcggcgccct gctcgtcggg ctggccaccg gcaagtctct ggcctaccgg cgcggcttgc
     1021 ccctcatccc cgtgaaccac ctgcaggggc acatcgccgc caactacgcg ctcggggtgg
     1081 aggcgccgtt cgtgtgcctg gtcgccagcg gcgggcacac gctgctcgcc gtggtcgagg
     1141 agggcgtgcg ctatcgggtc gccgccaaga ccatggacga cgcggccggc gaggcgttcg
     1201 acaagggcgc ccggctgctg gggctcggct acccgggcgg caaggagctc gacggcctgg
     1261 cggcgcgcgg agacccgggg ttcgcccgtt tcccgcgcgc cgtgcccagg ggccgtgggt
     1321 tcagcttcag cggcctcaag acggcgctac tctacgacct caggggcagg gaggaggcgg
     1381 agatcgccgc gcaccgcgcc gacatcgccg cttcgtacca ggccgccatc gtccgccagc
     1441 tggtggacaa gacgctggcc ggcgccgccg ccgagggcct gcgcaccgtc gccgtcgccg
     1501 gcggcgtggc cgccaattcg ggcctgcgcc gggccctgac cgaagcctgc gaggccgagg
     1561 gtctgcggct gtcgttgccg cccctcagcc tctgcaccga caacgccggc atgatcggcc
     1621 tggcggccgg gttcctgccg gcgatcccct ggccgggcta cctcgacctg gacgcgttcg
     1681 ccagcgacgc cgaggcaaag gcggcgcgcc cgccgcggaa gggctccgcc cgccgcgccc
     1741 cacgtcccta gccgttgccg ctcactcgac acgctgtccg tccgaacagc tcggactcgg
     1801 ccggccgtta gcctcggggc taggtgccgg aatgctagac tcggtcccag catgacgcac
     1861 cggcccgcat ccgtgtttcc gcccgcaccc ctcagcgacg cgccgcgccc ggcggcgaac
     1921 ctcttcgtcg actgtctcga gaacgagggc gtggagttcg tcttcggcat cccgggcgag
     1981 gagaccctcg acctcagcga ggcgctcgac cgctcgcagc tcatcacctt cgt
//
LOCUS       DASUMX010000206        11122 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_504544, whole genome shotgun sequence.
ACCESSION   DASUMX010000206 DASUMX010000000
VERSION     DASUMX010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 11122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11122
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504544"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            199..1632
                     /locus_tag="VFZ86_09990"
     CDS             199..1632
                     /locus_tag="VFZ86_09990"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HEX5642651.1"
                     /translation="MTVRVEWLTGAEREAVYAEAVAILERVGMKMKGAAVLPLLREAG
                     ARVDGDGVVRFPSELVERAVAACPREVLMAGETTASDVLLDGSRSFFNVSGCAAKTLD
                     RETGVVRPSTLADLRDGTIVLDATPELDVVWTFLTATDVPLERRELVEYYTYLTETAK
                     PVVFVDCPSETGHVRRIFETVSGDLERFRARPRVSLLCAARSPLEVNGGLLDVAVEFA
                     SLGSPVWAYSMPISGATAPVTPAGTLALLWAEVLGTITAIETAVPGAPVIACCGPGIL
                     DMRHTTMSLGNLESTLMGAASVEIGHHLGIPVHNSGLSTDAKHCGVQAGYEKGLKVMA
                     AVATGADIVSGGFGFLDSSSTFSLPMVPVDAEIAAMARRMARGIEISPETLMGDSFAR
                     VGIGGDFLREKETRRRVRAGEHYVPRIGSRLPFEQWLAEGRTEVDAAGDSVRAALAAR
                     AERGPRLTGEQRAELAGICGIGPEHAA"
     gene            1700..3166
                     /locus_tag="VFZ86_09995"
                     /pseudo
     CDS             1700..3166
                     /locus_tag="VFZ86_09995"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
     gene            3216..4577
                     /gene="mtbB"
                     /locus_tag="VFZ86_10000"
                     /pseudo
     CDS             3216..4577
                     /gene="mtbB"
                     /locus_tag="VFZ86_10000"
                     /inference="COORDINATES: protein motif:HMM:NF021053.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="[dimethylamine--corrinoid protein]
                     Co-methyltransferase"
     gene            4981..5709
                     /locus_tag="VFZ86_10005"
     CDS             4981..5709
                     /locus_tag="VFZ86_10005"
                     /inference="COORDINATES: protein motif:HMM:NF040370.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase family protein"
                     /protein_id="HEX5642652.1"
                     /translation="MNKIVPLWSVPRSISTGFERMMFERGDFKVIHEPYGYFFYLEAA
                     HKEPVGMQPQEGHPRTFEGTTEMIEAASADKPVFFKDMAYYVSDVADEDFMKKFTNTF
                     IIRDPALTLVSYHKLDPTVTLREIGFESQFKLFELAKKITGEVPAVVDAEDLLIDAGS
                     VVKQYCEKVGIPFLPESLTWEPEFKQEWKDWEMWHLDAGDSTGFQKDMEDFGYTVDDV
                     PELKEMYEQCKPLYEKMYEVRLRP"
     gene            5840..7066
                     /gene="sat"
                     /locus_tag="VFZ86_10010"
     CDS             5840..7066
                     /gene="sat"
                     /locus_tag="VFZ86_10010"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HEX5642653.1"
                     /translation="MNDHPDPTQGPAGLITPFGGKLVDLVVHDPDERAALLKRAQNLP
                     VVPLSLRAQYDLEMLAVGAFSPLDRFMAHDEYESVLYEMHLESGELFPIPLALPCREE
                     VLRGHPREIALSDEEGVILALLEVDEVYPADILREMRAVLGGRDSAHPLVIEAKGWPS
                     HYLAGRPRVFDLPRHRLHGPLCLTPAELRARLTAMGHSTVVAFQTRNPMHRIHEELTK
                     RALRTVGGTLLIHPAVGVTRPQDVAAALRVEAYEVLVRKYYDPSDTLLSLMPLAMRFA
                     GPREAVWHAIIRRNYGATHLIVGRDHAGPGPDSHGVPFYGPYDAQAMAEHYSHEIGVK
                     TILARELAYLTDEDRYVELNEVPYGARVSSISGTQVREEYLAKGKKLPEWFTRPEVAE
                     VLQRAYAQAGDASPGA"
     gene            7047..8726
                     /locus_tag="VFZ86_10015"
     CDS             7047..8726
                     /locus_tag="VFZ86_10015"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEX5642654.1"
                     /translation="MHRPAPDRGLLHDLVAGLTVGASQVGNAMAYTLLAGVPPVHGLY
                     AVAAGTSTGALAAGSQRMPMVPTAALCLAAGGALATLPPEQRTAGLFTLAVLTGAIML
                     VLGLLKAGGLVRFVSNAVMIGLMAGIGVQILLSQLGALTGFSSRYDNKILEAADLFIH
                     PGASDVETTAIGLATVLLVVVLRRTRLKLFAMALALLAMTAAVAALSLESVAVVGDIA
                     PIPRALPWPQLPDFSLVPSLLVPAASLVIIGLVQAAGISRTVPNADGSFGDTSRDFVG
                     HGVANIFSGVFSGAVVGGSVQATALNVGAGARTRRASVITAAFVVVVILLAAPLVQQV
                     PLAVTAGILIVAATSALQPRAARDVWRADKMSAAVMLVTFVLVLVVPLQYAVLAGATI
                     SVLKYIYLASLDVHVTQVVTDEDGRLREAEAPAALASGSVTVLDIYGSMFFAAAPKIR
                     ESLPSVGDARCPVVVLRLRGRGTLHSATIALLRDYAAECAANGGRLYLAGVGREMEDQ
                     LRRTGLLELLGADAVVAATDEIYGACALAQERGESWLAAHADPPPAGPAAP"
     gene            complement(8776..9624)
                     /locus_tag="VFZ86_10020"
     CDS             complement(8776..9624)
                     /locus_tag="VFZ86_10020"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX5642655.1"
                     /translation="MLAAVLALLASFSWGTSDFLAGLESRRSTAWTAALGGQAVAALS
                     LLALIVVVGPARPGLGALAAPAIGGVAGGLGVVLQYRALALVDMSLVSPIIAGAALVP
                     VLWGTASGERPGALQVVGIVLTLVGIVLISRKGRGAVTATARSFDRTGILMAVASAAA
                     FGFFLVALDHGGESDPLWTVTVARTAAVLALAAVAAVARPVIRLRRRALPALLLIGVL
                     IAAANFLFTSATTLGYLSVVGVLGWLNPAITMVWARLVLRERLGPLQLGAAALVFAGI
                     VCLTLG"
     gene            complement(9698..10288)
                     /locus_tag="VFZ86_10025"
     CDS             complement(9698..10288)
                     /locus_tag="VFZ86_10025"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX5642656.1"
                     /translation="MKGEMDGHPETQPYVRLGSLPRGTIELDDLDRRIMKLLRHDGRL
                     SYAHIARTVGLSEPTVRKRIDRLVSAGAIINAARINPAPIGFPIDAMIGLKVVRGRVK
                     EVGRKLAEMENVAYVAYVAGSFDIIIEAFLPHTEGLFKFLNEDLDRIDGIASTETWNV
                     LRTEKFFYNWEGEDVGLDLPPDETGPDRDDGRSPQP"
     gene            10649..>11122
                     /locus_tag="VFZ86_10030"
     CDS             10649..>11122
                     /locus_tag="VFZ86_10030"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5642657.1"
                     /translation="MAEQNDRSGYEVGDITRFINGTRVTRRQVLKGAAAIGALSALGP
                     LASACGGDETTTEPSASAAGTPNKGGDLMVGIVGGSAKDTADPHTGSFEPDIAIQYIM
                     YEGLTAWDFDLNLQNQLAESIEPNADGTVWQVKLRDGVMWHDGTPVTADDVVFSLD"
BASE COUNT         1703 a         3916 c         3924 g         1579 t
ORIGIN      
        1 ggacgagggc ctgcccttca agaccgtcgt cggcggcgcc ccctgcacgc agcgctgggc
       61 cgaccagatc ggcgccgacg cctacgccga ggacgccaac gacggcgtca agaagatcga
      121 cgcgctgctg gggaagtaag ctgtccccgg cgaggtcggg cgcccgcgag cgccaagggg
      181 cggacaaggg gtgatcggat gacggtgcgg gtggagtggc tcaccggcgc ggagcgcgag
      241 gccgtgtacg ccgaggcggt cgccatcctg gagcgcgtcg gcatgaagat gaagggcgcc
      301 gccgtgctgc ccttgctgcg tgaggcgggc gccagggtgg acggcgacgg tgtcgtcagg
      361 ttcccctccg agctggtcga gcgcgcggtc gcggcgtgcc cgcgcgaggt cctcatggcc
      421 ggcgagacga ccgcgtccga cgtgctcctc gacgggtcgc gatcgttctt caacgtcagc
      481 ggctgcgccg ccaagacgct cgacagagag accggcgtcg tacggccgtc cacgctcgcc
      541 gacctgcgcg acggcaccat cgtcctggac gccacgccgg agctggacgt cgtctggacg
      601 ttcctcacgg cgaccgacgt gccgctcgag cggcgggagc tggtcgagta ctacacctac
      661 ctcacggaga cggccaagcc ggtcgtcttc gtcgactgcc cgagcgagac cggccacgtg
      721 cgccggatct tcgagacggt ctccggcgac ctcgagcgct tccgcgcgcg gccgcgcgtg
      781 tcgctgctgt gcgcggcccg ctcgcccctc gaggtgaacg gcgggctgct cgacgtcgcc
      841 gtcgagttcg cctctctggg ctcgccggtc tgggcgtact ccatgccgat ctcgggggcc
      901 acggcgccgg tcacgccggc ggggacgctg gcgctcctgt gggcggaggt gctcggcacc
      961 atcaccgcga tcgagacggc cgtccccggg gcgcccgtga tcgcctgctg cggccccggc
     1021 atcctcgaca tgcgccacac gacgatgtcg ctcggcaacc tcgagagcac gctgatgggc
     1081 gccgcctcgg tcgagatcgg ccaccatctc ggcatccccg tgcacaactc aggcctctcg
     1141 acggacgcca agcactgcgg cgtgcaggcc ggctacgaga agggcctcaa ggtgatggcg
     1201 gccgtcgcga cgggcgccga catcgtcagc ggcggcttcg ggttcctcga ctcgtcgagc
     1261 acgttcagcc tgcccatggt gccggtcgac gccgagatcg ccgccatggc gcggcgcatg
     1321 gcgcgcggga tcgagatctc gcccgagacg ctgatgggcg actcgttcgc gcgcgtgggc
     1381 atcggcggcg acttcctccg tgagaaggag acgcgccggc gcgtgcgcgc cggcgagcac
     1441 tacgtgccgc ggatcggctc ccggctgccg ttcgagcagt ggctcgccga gggcaggacc
     1501 gaggtggacg ccgccggaga ctcggtgcgg gcggcgctcg ccgcgcgtgc cgagcgaggg
     1561 ccgcggctga ccggcgagca gcgcgccgag ctcgccggca tctgcgggat cgggccggag
     1621 cacgcggcat gaccgggccg gagaagcgcc cgaccacgaa ggaaccagac cacaccacag
     1681 agacgaggga ggtgcgcccg tgagacgcaa tgcccatgcg ggacgcgcac gccgcgacgg
     1741 ctgcgagttc cgcgtactga ccgacgacga cctcgccgac atccatctgg ggacgctcga
     1801 ggtcctcgag cggacgggcg tgttcgtcga ggacgaggag gcccgcgaga tcttcgccgg
     1861 ggccggcgcc acggtcgacg ccaggggcgt cgtgcgcatc cccggccacg tcgtcgagca
     1921 ggccatccgc acggcgccca gcaagctccg gctcgccggc cgcagtccca ggaacgacat
     1981 cgtgctcgag gccggccgcg tcggcttcac caacttcggc gagggcgtcg cgatcatcga
     2041 tccctacacg ggcgagctca aggtgccgac caagcaggac gtcgccgcta cggccaaggt
     2101 cgtggacgcg ctgcccaacg tggacgtcta cgagcgcgcc gtggccgccg aggacatcga
     2161 ccagcgggtc gtccacctgc accagtacga ggcctgggcg accaatacct cgaagcacgg
     2221 cttcatgggc tccggcaacg gctggctctc ccgcaagctg gtcgagatgg ccgccgccat
     2281 cgccggcggg cgcgagcagc tcaaggcgcg cccgatcatc agcttcatca cctgcccggt
     2341 gagcccgctc aagctggtgc aggacacctg cgagatcatc atgaccggcg cgcgcgagca
     2401 cgtggccgtc aacatcctct cgatggcgat ggccggcggc tcgtcgccgg tgacgctcgc
     2461 cggcacgctg gtgacgcaca acgccgaggt gctcgccggc atcaccctgg gccagctcac
     2521 ccggccgggt gcgggggccg tgtacggcag ctccaccacg gccatggacc tgcgcctggc
     2581 ctcggccagc gtcggctcgc ccgagtgcgg cctcatcagc gcggcggtcg cccagatcgc
     2641 gcgcttctac ctgctcccca gctgggtcgc cggtgcgtag ggcgacgcca agacggtcga
     2701 tgcccaaagc ggccacgaga agaccctgac cggcctgatg ccggcgttgg caggcgccaa
     2761 cctgatctac ggtctgggga tgatcgagtc gggcatgacc ttcgacttcg gccagctcgt
     2821 catggacgac gagttcgccg ggatgatcaa gcagtgtgtg cgcggcatcc gcgtggacga
     2881 cgagacgctc gcgctcgacg tgatcgacga catcggtccg ttcggcgact tcctcagcca
     2941 cgaccacacg atggcgcaca tgcgcgagca gtcgcagccc gcggtgatcg accgtcgcgt
     3001 ccgggaggac tgggcggcga gcggttccct caccctgcag gaccgcgccc tcgccaaggc
     3061 gcgcacgatc ctcgagaccc acgagccgga cccgctgccc gacggcgtgg ccgacgagct
     3121 cgcggcgatc atccgtgggg ccgagcgcga gctcggcatc gcctgacgat gggttccgga
     3181 gaggaggcgc cgaggtgagc accatccgca cgcgcatggg cgacggctac gtgaccgagc
     3241 tcacgccgca ggagctgcgc gacgatctcg tggccggttc ggccgacgcg gtcaagcgcg
     3301 gcaaggtccc gccgctcgag gagaacgaga tcgactggct ggtggacctg ttcaccaacc
     3361 ccagccgcat catgggcgtc gagcccggcc acgaggtcat cctgaccaag gacggctcgg
     3421 tcaacacgct ctactcggcg cagctctcga gcggcgtggg catcggcctg tcgcgcgagc
     3481 agtgcttccg catcttcgag cgcgccttct gcttcgacac catggagatc ggccacccgg
     3541 actactccta caagccggtc aagccgatcg tcgccctcga ggcgatcggc gtggagcagg
     3601 cgctgctcac cgccgtcatc ccggtgttct acggggcgat gcccaacttc ggcctctacg
     3661 cgcagccgga cgggccgttc cccaatccgg ccgatctgct cccgcgcggc gagatcgcgg
     3721 ccgcgcgcga ggcgcagatg cagatggccg cggcctgcaa ggccgacatg ctctacgtct
     3781 cgcgcatgat ggccgaggtg ggcagcgacg gcatcaactt cgacaccacc gcctcgaccg
     3841 gcgacgccga gttcctcgcc gtgctggaga cggtcgagga gctggccgcg accaccgacc
     3901 tcgcgatcga ggtcgggatg gcggccgagt tcgtgctcgg cttccacggc gagctcgagt
     3961 accacggcaa gcggctcgcc ggcatgtggc cgcaccagca ggtcaaggtc gtcgaggagg
     4021 cgggcgcgca cctgttcggg ccggtcgtga acaccaactc gcgccggagc acgccctgga
     4081 acgtggcccg cgcggtcacg atcgtcaagg agtgcagccg ggtcgcgacc atccccatcc
     4141 acgccaacgt cggcatgggc gtgggcggcg tgccgatgtt cgaggtgccg ccggccgacg
     4201 tgctcacgcg cgcgagcgcg gccatggtcg agatcggccg ggtcgacggc ttgtaggtgg
     4261 gcaccggcga tccctacggg atgtcgatca gccacgcgct cgcctccggc atggggggca
     4321 tccgcaccac tggagatctg gtggccagga tgcagctccg caggaagatg aggctccccg
     4381 aggcgaagaa gtacgtggcc gacaagctca gggtcggcgt ggaggacctc gccgactcga
     4441 cggtgatgcg gggcctgcgc gaggagctcg acatcggcgt catcaccgcg gtgcccggcg
     4501 cgcgcaaggg tatcgaggcc aagcgcaaca tcgccgagct gctcgacgtc gaggtgccct
     4561 gcctcaagcg cctgtagagc cagacggctg ccctctcgcg aggggtgtgc ccaccgcccc
     4621 gtcccgcgcc gaaggcgcgg ggcggggcgg tctgcgcagg cgcgcccgcg cgcccgcgcg
     4681 ccgcgcgcgc tgccgccggg atgttttaga ttccgaagcg attcggcctt caggattagt
     4741 aagtaccgat tgagagtcct cttcgtgcgg ttgagggcca gaatcggtga gaatcgctgt
     4801 gattccgtca cgtgaggctt gccaggacgc gagcccggtg ctagtctcgg atgcggggat
     4861 cagggtgtcg ccacggcggc ccggcgaggc cgtcgcccag cacggcgctt cggtatcccc
     4921 ggagccgcga cggcggatcc agcagtgctc ggcacacacc actgatcgag gagcagccac
     4981 atgaacaaga tcgtccccct ctggtccgtc ccgcggtcca tctcgacggg cttcgagcgc
     5041 atgatgttcg agcgcggcga cttcaaggtc atccacgagc cgtacggcta cttcttctac
     5101 ctcgaggccg cgcacaagga gcccgtgggc atgcagccgc aggagggcca tccgcggacc
     5161 ttcgagggca cgaccgagat gatcgaggcc gcctccgccg acaagccggt gttcttcaag
     5221 gacatggcgt actacgtcag cgacgtcgcg gacgaggact tcatgaagaa gttcaccaac
     5281 acgttcatca tccgcgaccc cgccctcacg ctggtctcgt accacaagct cgaccccacg
     5341 gtgacgctgc gtgagatcgg cttcgagtcg cagttcaagc tgttcgagct cgccaagaag
     5401 atcaccggcg aggtgccggc ggtcgtcgac gccgaggacc tgctcatcga cgccgggtcc
     5461 gtggtcaagc agtactgtga gaaggtcggc atcccgttcc tgcccgagtc gctcacctgg
     5521 gagcccgagt tcaagcagga gtggaaagac tgggagatgt ggcacctcga cgccggcgac
     5581 agcaccggct tccagaagga catggaggac ttcggctaca cggtcgacga cgtgccggag
     5641 ctcaaggaga tgtacgagca gtgcaagccg ctgtacgaga agatgtacga ggtgcgcctg
     5701 cgtccgtgac gaggctcatc cacccgggag ggcctgtgtg caacaggcct tcccgggggg
     5761 atccgtccgt ggccacgccc gctccgcccg gtaccgtgtg aggacccgaa ccgcaccacc
     5821 gcgggcgagg agctctgccg tgaacgatca tccagatccg acccaaggcc ccgcaggcct
     5881 catcacgccg ttcggcggca agctggtcga cctggtggtc cacgaccccg acgagcgcgc
     5941 cgccctgctc aagcgggccc agaacctccc cgtggtcccg ctctcgctgc gcgcccaata
     6001 cgacctcgag atgctggccg tcggcgcgtt ctcgccgctg gacaggttca tggcgcacga
     6061 cgagtacgag tcggtcctct acgagatgca cctcgagagc ggcgagctgt tcccgatccc
     6121 gctcgccctt ccctgccgcg aggaggtcct gcgggggcac ccgcgcgaga tcgcgctctc
     6181 cgacgaagag ggggtcatcc tcgccctgct cgaggtggac gaggtctacc cggccgacat
     6241 cctgcgcgag atgcgcgcgg tactcggcgg cagggacagc gcgcatccgc tcgtcatcga
     6301 ggccaaaggc tggccgagtc actacctcgc cggccgcccg cgcgtgttcg acctgccgcg
     6361 ccaccggctg cacgggcctc tctgcctgac gcccgcggag ctgcgcgccc gcctcaccgc
     6421 catgggccac tcgaccgtgg tggcgttcca gacgcgcaac ccgatgcacc gcatccacga
     6481 ggagctcacc aaacgcgcgc tgcggacggt cggcgggacg ctcctcatcc atccggccgt
     6541 gggcgtcacc cggccccagg acgtggccgc cgcgctccgc gtggaggctt acgaggtgct
     6601 cgtgcgcaag tactacgatc cgtcggacac gctgctctcc ctgatgcccc tggccatgcg
     6661 cttcgcgggg ccgcgggagg cggtgtggca cgccatcatc cggcgcaact acggcgccac
     6721 ccacctcatc gtcgggcgtg accacgcggg ccccggcccg gactcgcacg gcgtgccctt
     6781 ctacgggccc tacgacgcgc aggcgatggc cgagcactac tcgcacgaga tcggggtcaa
     6841 gaccatcctc gcgcgggagc tcgcctacct caccgacgag gaccgctacg tggagctcaa
     6901 cgaggtgccg tacggcgcgc gcgtctcgtc catctccggc acccaggtgc gcgaggagta
     6961 cctggccaag ggcaagaagc tgcccgagtg gttcacgcgg ccggaggtcg cggaggtcct
     7021 gcagcgggcg tacgcgcagg ccggcgatgc atcgcccggc gcctgaccgc ggcctcctgc
     7081 acgacctggt cgcgggcctc acggtagggg cctcccaggt cggcaacgcc atggcgtaca
     7141 cgctgctcgc cggcgtgccg ccggtccacg gcctgtacgc cgtcgccgcc ggtacgtcga
     7201 cgggggcgct cgccgcaggc tcccagcgca tgccgatggt cccgacggcc gcgctctgcc
     7261 tcgcggccgg cggcgcgctg gcgacgctgc cgcccgagca gcgcacggcc ggcctgttca
     7321 cgctggcggt gctcaccggc gccatcatgc tggtcctggg gctcctcaag gccggcggac
     7381 tcgtgcgctt cgtctccaac gcggtgatga tcggcctgat ggccggcatc ggcgtgcaga
     7441 tcctgctctc gcagctcggc gcgctcaccg gcttctcgag ccggtacgac aacaagatcc
     7501 tcgaggccgc cgacctgttc atacaccccg gagcctccga cgtggagacc accgccatcg
     7561 gcctggccac cgtgctcctg gtcgtggtgc tcaggcgcac ccgcctcaag ctgttcgcga
     7621 tggcgctggc gctgctggcg atgacggcgg ccgtcgccgc gctgtcgctc gagtccgtgg
     7681 cggtcgtggg cgacatcgcg cccattccgc gagcgctgcc ctggccgcag ctgcccgact
     7741 tctcgctggt cccgtcgctg ctggtgccgg cggcctccct ggtgatcatc ggcctcgtcc
     7801 aggccgccgg gatcagccgc acggtcccca atgccgacgg ctcgttcggg gacaccagca
     7861 gggatttcgt cgggcacggg gtggcgaaca tcttctccgg cgtcttcagc ggcgcggtgg
     7921 tgggaggctc cgtgcaggcc accgccctca acgtcggagc cggcgcgcgg acgcgccgcg
     7981 cgtcggtgat caccgccgcc ttcgtggtcg tcgtgatcct tctggcggcg ccgctggtgc
     8041 agcaggtgcc gctggcggtg acggcgggga tcctcatcgt cgccgcgacg agcgcgctgc
     8101 agccgcgggc cgcgcgcgac gtctggcgcg cggacaagat gtccgccgcc gtcatgctcg
     8161 tgaccttcgt cctcgtcctc gtcgtccccc tgcagtacgc ggtgctcgcc ggggcgacga
     8221 tctccgtgct caagtacatc tacctggcgt cgctcgacgt ccacgtcacg caggtcgtga
     8281 cggacgagga tgggcgcctg cgggaggcgg aggccccggc cgcgctggcg agcggcagcg
     8341 tcaccgtcct ggacatctac ggcagcatgt tcttcgcggc cgcccccaag atcagggagt
     8401 cgctcccctc cgtcggcgac gcgcgctgtc ccgtcgtggt gctgcgcctg cgcggccgcg
     8461 ggacgctgca cagcgccacg atcgcccttc tccgcgacta tgcggccgag tgcgcggcga
     8521 acggggggcg gctgtatctc gcgggagtcg gtcgcgagat ggaggaccag ctgcgccgca
     8581 ccggcttgct ggagctgctg ggggcggacg cggtggtcgc ggcgaccgac gagatctacg
     8641 gcgcctgcgc gctcgcccag gagcgcgggg agtcgtggct ggccgcgcac gccgacccgc
     8701 cccccgccgg cccggccgcg ccctgaggtc gcgtcggccg tcgccggcgc gaggcgcagg
     8761 gaggacgccg gcgcctcagc cgagcgtgag gcagacgatg ccggcgaaca ccagtgcggc
     8821 cgcgcccagc tggagcggcc cgaggcgctc acggagcacg agcctcgccc agaccatggt
     8881 gatggcgggg ttgagccacc cgaggacccc gacgacgctg agatagccga gggtcgtcgc
     8941 cgaggtgaac aggaagttgg cggcggcgat caggaccccg atgaggagga gggccggcag
     9001 cgcgcggcgg cgcagccgga tgaccggccg ggcgacggcg gccacggcgg ccagcgccag
     9061 caccgccgcc gtgcgcgcga ccgtcaccgt ccacagcggg tcggactcgc cgccgtggtc
     9121 gagggcgacc aggaagaagc cgaaggcggc cgccgaggcc acggccatga ggatgccggt
     9181 gcggtcgaac gagcgcgccg tcgccgtgac ggcgccacgt cccttgcgcg agatgaggac
     9241 gatccccacg agcgtgagca cgatgccgac gacctggagg gcgccgggcc gctcgccgct
     9301 cgccgtaccc cagagaacag gcacgagagc cgcgccggcg atgatcggcg agaccaggct
     9361 catgtcgacg agcgccagcg cgcggtactg caggacgacg ccgaggccgc cggccacgcc
     9421 gccgatcgcc ggcgccgcca gggcgccgag accgggccga gccggtccga cgaccacgat
     9481 cagggcgagc aacgagagcg ccgcgaccgc ctgaccgccg agcgccgccg tccaggcggt
     9541 cgagcggcgc gactcgaggc cggcgaggaa gtcggaggtg ccccacgaga agctggcaag
     9601 gagggcgagg acggcggcca gcattcaggt cctgggggaa gtcactcggg ggcgcgcgac
     9661 ccgggatgaa gcaggaagcc ggccgcgcca gctgagctca aggttgcgga gagcggccgt
     9721 cgtcccggtc cggcccggtc tcgtccggtg ggaggtcaag gccgacgtcc tcgccctccc
     9781 agttgtagaa gaacttctcg gtgcgcagca cgttccaggt ctcggtgctg gcgatgccgt
     9841 cgatcctgtc gaggtcctcg ttgaggaact tgaacaggcc ttcggtgtgg ggcaggaacg
     9901 cctcgatgat gatgtcgaag ctgccggcga cgtacgcgac gtacgcgacg ttctccatct
     9961 ccgccagctt gcggcccacc tccttgacgc gcccgcgcac caccttgaga ccgatcatcg
    10021 cgtcgatggg gaagccgatg ggcgccgggt tgatgcgggc cgcgttgatg atcgcgcctg
    10081 cgctcaccag gcgatcgatg cgcttgcgca cggtcggttc ggagaggccc accgtgcgcg
    10141 cgatgtgcgc atagctcagc cgcccgtcgt ggcgcagcag cttcatgatc cgccggtcga
    10201 ggtcgtcgag ctcgatggtg ccgcgcggca acgagcccag gcggacatag ggctgcgttt
    10261 cgggatgacc atccatttcg cctttcatag atgcgaagta tagcgattcc acgcaactcg
    10321 ttcaatgaat cggtcggaac acgttggttg cgctgtgatt ccatcatcat gccttgtcag
    10381 ctcagcaccc gggtgctacc ctgcgagtgt cgttcattga caatctggta gcgtgggcct
    10441 agagtccaat ccaagactgg cgacccaccc aggtggataa atctactcga cggatctggg
    10501 gaggtccgtg ggccgccgtt gatctcggcc aagggggttc gtctaacgca agttcacgtg
    10561 acgccgggat acgggtgatc ggccgtccga ggacggcgct cttccgtgtc ccgcctgcag
    10621 tgtaagaaca ggaatggggg acggcatcgt ggcagaacag aacgacaggt cagggtacga
    10681 ggtcggggac atcacccgct tcatcaacgg cacgcgggtc acccgccgcc aggtactgaa
    10741 gggggctgcc gccatcggcg cgctctccgc cctcgggccg ctggcgtcgg cttgcggcgg
    10801 ggacgagacc accacggagc cctccgcctc cgcggcgggc accccgaaca agggcggcga
    10861 cctcatggtc ggcatcgtcg gcggctcggc caaggacacg gccgatccgc acaccggctc
    10921 gttcgagccc gacatcgcca tccagtacat catgtacgag ggtctcaccg cctgggactt
    10981 cgacttgaac ctccagaacc agctggccga gtccatcgag cccaacgccg acggcacggt
    11041 ctggcaggtc aagctgcgcg acggggtgat gtggcacgac ggaacgcccg tcaccgccga
    11101 cgacgtcgtc ttctccctgg ac
//
LOCUS       DASUMX010000207         6538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1799268, whole genome shotgun sequence.
ACCESSION   DASUMX010000207 DASUMX010000000
VERSION     DASUMX010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 6538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6538
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1799268"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VFZ86_10035"
     CDS             complement(<1..188)
                     /locus_tag="VFZ86_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX5642658.1"
                     /translation="MKTAYFDHAATTPVDPRVLEAMLPWFSERYGNPSELHRLGREAR
                     AAVEAARAQVAGAHGAGD"
     gene            complement(185..598)
                     /locus_tag="VFZ86_10040"
     CDS             complement(185..598)
                     /locus_tag="VFZ86_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008339414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RrF2 family transcriptional regulator"
                     /protein_id="HEX5642659.1"
                     /translation="MRISSRCEYGLRAMVYLAGHDSGRPVPLPEIAAGESIPAPFLER
                     ILARLREAGLVKATRGVSGGYQLARPAADVAVGDVVTALEGPLSLVGCVPDDGACARA
                     DSCASRIVWRRLDSAISGALNGITLEDLTREAVSP"
     gene            complement(733..1269)
                     /locus_tag="VFZ86_10045"
     CDS             complement(733..1269)
                     /locus_tag="VFZ86_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642660.1"
                     /translation="MGIFKRNRTFLLGAVLILVIGALLFGGVIAVRVMAESSATPAPA
                     ATQPASYNSVSSYGMTGYPALTLEPTAETPDFITKRLDEGRGIVLLVYVKGASDDMEM
                     LSSFNDIKANYAADSSFFSFEARESKQLGDTLMQLRVSDPPILAIIRGDGTVANMYTG
                     WIGFKVMEQEIADAVRGL"
     gene            complement(1293..1446)
                     /gene="ssrS"
                     /locus_tag="VFZ86_10050"
     ncRNA           complement(1293..1446)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VFZ86_10050"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(1556..3319)
                     /gene="aspS"
                     /locus_tag="VFZ86_10055"
     CDS             complement(1556..3319)
                     /gene="aspS"
                     /locus_tag="VFZ86_10055"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011232049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEX5642661.1"
                     /translation="MLRDTYPGELRAADVGREVRLAGWVARRRDHGGLIFIDLRDRSG
                     MVQLVVDPEHAAEAHALAEQVRSEFVLAVTGAVVARSPETVNPKLPTGEVEVQVAGLE
                     ILNRAKTPPFEIETAEQVDVDETLRLRYRYLDLRRAPMLANLELRNDATQAARAYLNA
                     NRFIEVETPDLTKSSPEGARDFLVPSRLQPHRFYALPQSPQMFKQILMVAGLDRYYQF
                     ARCFRDEDLRADRQPEHTQIDLETSFLTVPEIRELLEGLMTAIFEGVGETAPATPLPR
                     LSYDEAMLRYGSDKPDLRYGFEIVELSETFAQSEFKVFRGAVDGGGVVRAIRAPGGGS
                     LSRAQIDKLTELAKEHGAKGMAYVYVEEGRALRGPIVKFLAEAEQAALVEKVGGEPGD
                     LVVFAAGEALVAAEVLGALRLALIGLLAPEPSARWSLLWVEEFPLFEIDKETGTLTYG
                     HNPFSLPTAATMPLMESDPLAVRGAQYDLVLNGVELGSGSLRNHDAAVQRQILRALGM
                     SDERIDEAFGWFLEALEYGAPPHGGIGLGFDRVIMLLAGADSIRDVIAFPKTSSGGDP
                     MTGAPGPVAAEQLKELRVRLG"
     gene            complement(3319..4635)
                     /gene="hisS"
                     /locus_tag="VFZ86_10060"
     CDS             complement(3319..4635)
                     /gene="hisS"
                     /locus_tag="VFZ86_10060"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEX5642662.1"
                     /translation="MTAPRAPKGTYDLLPDAAAERDAVVAAAARVFAIYGYRHVVTPE
                     FEETGLFERGVGEATDIVRKEMYTFTDKGERMLTLRPEGTAPVCRAYVEHGMHKLPQP
                     VKLWYYCPMFRYEKPQAGRYREHYQIGAEAIGSAEPAVDAEVILLLAAIFAELGVEGV
                     TLNMNSMGDPQCRPAYVEKLREYLRGREKELCGDCLERIDLNPLRTFDCKVESCRAVL
                     EEAPRISDHLCPECREHFDAVLALVRAAGLEPNLDFRLVRGLDYYTRTTFEFNSDRLG
                     AQSGVGGGGRYDGLVEQIGGPPTPGVGWGSGLERIALAMREPLAVPASTGVYVVAFGD
                     DARPAAFACAQKLRRAGVPAELDLAGRSPKGQLKQAGRSGARFAVLLGLEELAGEVVR
                     LKDLAGGGEEDVGAADLPALVAARLAGVAAPASGAPPAHRSLESET"
     gene            complement(4632..5282)
                     /locus_tag="VFZ86_10065"
     CDS             complement(4632..5282)
                     /locus_tag="VFZ86_10065"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5642663.1"
                     /translation="MPEPVEVFAVPLGPLQANCFIVVQGEQGLVIDPGDEANVVIEAF
                     TEFGFAPAAVLVTHGHFDHFGGVTPVARHFDIPVYVGAEDAGQMADGGLGSMAGFDIE
                     PVETARTIEGEQTLELPITVLAIPTPGHSRGSYTFAIGEEELFVGDLLFQSSVGRTDL
                     PGGDMDQLLNSVAALIRRFPPDATVHCGHGPDTSLGRELALNPFLSPIRHDPEHRW"
     gene            complement(5421..>6538)
                     /locus_tag="VFZ86_10070"
     CDS             complement(5421..>6538)
                     /locus_tag="VFZ86_10070"
                     /inference="COORDINATES: protein motif:HMM:NF040246.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HEX5642664.1"
                     /translation="LAWLHQMMEWQSETGDPREFMDTLRVDLFEDEVFVFTPKGDVKS
                     LSAGSTPIDFAFAVHTDVGNHTVGAKVNGRIVPLHTKLSSGDIVEVITSRSSRGPSRD
                     WLDIVNSPRARQKIRQHFRREQREDSEQSGRDLLQEALRREGLPAQKILSSKVFAQIC
                     KDVGYSKPDDLYVALGSGRLPVKTVANKVMQRAGTMKEATPPPEMLPSRPVQDAEEPQ
                     ALSGEFGIAVEGMSDIMVRMAKCCKPIPGDEIVGYISLGKGITIHRKDCKNARALMKN
                     PERFTKVGWKGLGGMAFRVEIMIEALDRNHLLEDVARTLSDSGVNIIGGSVQTLPDGV
                     VRDRFTLEVGDVRQLDNILANIRSIQTVYDAYRVVGG"
BASE COUNT          953 a         2237 c         2361 g          987 t
ORIGIN      
        1 ttgtcgccgg cgccgtgggc gccggcgacc tgggcgcgcg ccgcctcgac ggccgcgcgc
       61 gcctccctgc ccagccggtg cagctcggac gggttgccgt agcgctccga gaaccaggga
      121 agcatcgcct cgagcacccg aggatcgacc ggcgtcgtgg ccgcgtggtc gaagtaggcc
      181 gtcttcatgg tgacactgcc tcccgcgtga gatcctcgag ggtgatgccg ttgagcgccc
      241 cgctgatggc gctgtccaga cgccgccaga cgatgcgcga cgcgcacgag tcggcgcgag
      301 cgcaggcgcc gtcgtcgggc acgcagccga cgagcgacag cgggccctcc agcgccgtga
      361 ccacgtcgcc gacggcgacg tcggcggcgg gacgcgccag ctgatagccg ccgctgaccc
      421 cgcgcgtcgc cttcacgagg cccgcctcac gcagccgcgc gaggatgcgc tcgaggaacg
      481 gagcggggat gctctcgccg gcggcgatct ccggcagcgg caccggccgg ccgctgtcgt
      541 ggccggccag atagaccatg gcccgcagtc cgtactcaca gcgcgatgag atgcgcacga
      601 ggctccctcc gctcagtggc ggcgaatccc aaccaataga gtcggcatta tgctaccacg
      661 gcggtcggga cggtcaaaca gaagcggcgc cccggagggc gccgcctgga gtcgctcgtc
      721 tggtgagaac ggtcagaggc cgcggaccgc gtccgcgatc tcctgctcca tgaccttgaa
      781 gccgatccag cccgtgtaca tgttggccac cgtgccgtcg ccgcggatga tggcgaggat
      841 gggcggatcg ctgacgcgca gctgcatcag ggtgtcgccg agctgcttgg actcgcgcgc
      901 ctcgaaggag aagaacgagc tgtcggcggc gtagttcgcc ttgatgtcgt tgaaggagga
      961 gagcatctcc atgtcgtcgc tggcgccctt gacgtagacg agcaggacga tgccgcggcc
     1021 ctcgtccagg cgcttggtga tgaaatcggg agtctcggcc gtcggctcga gggtgagcgc
     1081 cgggtaaccg gtcatgccgt agctgctgac cgagttgtac gatgccggct gggtggcggc
     1141 gggcgccggc gtggcgctcg actccgccat cacgcgaacg gcgatgacgc cgccgaacag
     1201 cagcgcgccg atcacaagga tcaggaccgc ccctagcagg aacgtccggt tgcgcttgaa
     1261 gatccccatg ccggcctccc ccctcagaga gaaagagctc caccttgccg tgctcgcgtt
     1321 attctcgaac ctgctctcgc aggtgggtgc cctcccggaa cgtccacgga cggtccggcg
     1381 aacgagctcc cgatgtgttc gtgttggctc gagaatgtgg cgaggtcacg aacaaggtcg
     1441 agccgcgcca tcctagcaaa cgtccactga ttcaacaatc ttgtcggcag ccctgcaaaa
     1501 tgcttgaggc cgaggccggg acaggcgtgg cctgagctga cgcgggtcgc ggccgctacc
     1561 cgaggcggac ccgcagctcc ttgagctgct cggccgccac aggccccggc gcgcccgtca
     1621 tggggtcgcc gccggaggac gtcttgggaa aggcgatgac gtcgcggatg gagtcggccc
     1681 cggcgagcag catgatgacg cggtcgaagc cgaggccgat gccgccgtgc ggcggcgccc
     1741 cgtactcgag cgcctcgagg aaccagccga aggcctcgtc gatacgctcg tcgctcatgc
     1801 ccagggcgcg caggatctgg cgctggacgg cggcgtcgtg gttgcgcagg gaaccggagc
     1861 ccagctcgac gccgttcagc accaggtcgt actgcgcgcc gcgcaccgcg agcgggtcgc
     1921 tctccatgag cggcatggtc gccgccgtcg gcaggctgaa cgggttgtgg ccgtaggtga
     1981 gcgtgcccgt ctccttgtcg atctcgaaga gcgggaactc ctcgacccag agcagtgacc
     2041 agcgcgcgga cggctcgggc gcgagcaggc cgatgagggc gaggcgcagc gcgccgagca
     2101 cctcggcggc cacgagcgcc tcgccggcgg cgaagacgac caggtcgccg ggctcgccgc
     2161 cgaccttctc gacgagcgcg gcctgctcgg cctcggcgag gaacttgacg atgggaccgc
     2221 gcagagcgcg gccctcctcg acgtacacgt aggccatccc cttggcgccg tgctccttgg
     2281 cgagctccgt gagcttgtcg atctgggccc ggctgaggga accgccgccg ggcgcccgga
     2341 tggcgcgcac caccccgccg ccgtcgacgg cgccgcggaa gaccttgaac tcgctctgcg
     2401 cgaacgtctc gctgagctcg acgatctcga agccgtagcg caggtccggc ttgtcggagc
     2461 catagcgcag catcgcctcg tcgtaggaga gcctgggcag cggcgtcgcg ggggcggtct
     2521 cccccactcc ttcgaagatc gccgtcatga ggccctcgag cagctcgcgg atctcgggca
     2581 ccgtgaggaa cgacgtctcg aggtcgatct gcgtgtgctc cggctggcgg tcggcgcgca
     2641 ggtcctcgtc gcggaagcag cgcgcgaact ggtagtagcg gtccaggccg gcgaccatga
     2701 ggatctgctt gaacatctgc ggcgactgcg gcagcgcgta gaaacggtgc ggctggaggc
     2761 gcgatgggac gaggaagtcc cgcgcgccct ccggagacga tttcgtgaga tccggcgtct
     2821 ccacctcgat gaagcggttc gcgttgagat acgcgcgagc cgcctgggtc gcgtcgttgc
     2881 gcagttctag gttggccagc atgggcgcgc ggcgcaggtc cagatagcgg tacctgaggc
     2941 gcagcgtctc gtccacatcg acctgctcgg ccgtctcgat ctcgaacgga ggcgtcttgg
     3001 cgcggttcag gatctcgagc ccggcgacct ggacctcgac ctcgccggtc gggagcttgg
     3061 ggttcaccgt ctccggcgag cgcgcgacga cggcgcccgt cacggcgagc acgaactcgg
     3121 accggacctg ctcggcgagc gcgtgcgcct cggcggcgtg ctcggggtcg acgaccagct
     3181 ggaccatgcc gctgcggtcg cgcaggtcga tgaagatgag gccgccgtgg tcacggcggc
     3241 gagccaccca gccggcgagg cgcacctcgc ggccgacgtc ggcggcgcgc agctcgccgg
     3301 gataggtgtc gcgcagcatc aggtctcact ctccagtgaa cggtgcgccg gcggtgcccc
     3361 gctcgctggc gcagctacgc cggccagtcg ggcggcgacg agcgccggca ggtcggcggc
     3421 gccgacgtcc tcttccccgc cgcccgccag atctttgagc cgcacgacct cgccggcgag
     3481 ctcctcgagc ccgagcagca ccgcgaagcg ggctccgctg cggcccgcct gcttgagctg
     3541 gcccttgggg ctgcgcccgg cgaggtccag ttccgccggc acgccggcgc ggcgcagctt
     3601 ctgggcgcag gcgaacgccg ccgggcgggc gtcgtcgccg aaggcgacga cgtagacgcc
     3661 ggtcgaggcg gggaccgcga gcggctcgcg catcgccagg gcgatgcgct cgaggccgct
     3721 gccccagccc acgcccggcg tcggcgggcc gccgatctgc tcgacgagcc cgtcgtaacg
     3781 gccgccgccg cccacgccgc tctgggcgcc gaggcggtcc gagttgaact cgaaggtcgt
     3841 gcgcgtgtag tagtccaggc cgcgcacgag ccggaagtcg aggttgggct cgagcccggc
     3901 cgcccggacc agcgcgagca cggcgtcgaa gtgctcgcgg cactccgggc acaggtggtc
     3961 gctgatgcgc ggggcctcct cgagcaccgc gcggcagctc tccaccttgc agtcgaacgt
     4021 gcgcagcggg ttgaggtcga tgcgctccag gcagtcgccg cagagctcct tctcgcggcc
     4081 gcggaggtac tcgcggagct tctcgacgta ggcgggccgg cactgcggat cgcccatgct
     4141 gttcatgttg agcgtgacgc cctcgacgcc cagctcggcg aagatcgccg ccagcaggag
     4201 gatcacctcc gcgtccacag ccggctcggc ggagccaatg gcctccgcgc cgatctggta
     4261 gtgctcgcgg tagcgcccgg cctgcggctt ctcgtaacgg aacatcgggc agtagtacca
     4321 gagcttcacc ggctgcggca gcttgtgcat gccgtgctcc acgtaggcgc ggcagacggg
     4381 cgccgtgccc tccggacgca gcgtcagcat gcgctcgccc ttgtcggtga acgtgtacat
     4441 ctccttgcgc acgatgtccg tcgcctcgcc gacgccgcgt tcgaagagac cggtctcctc
     4501 gaactcgggc gtgacgacgt ggcggtagcc gtagatggcg aagacacggg ccgcggcggc
     4561 gacgacggcg tcgcgctccg cggccgcgtc ggggagcagg tcgtaggtgc ccttgggggc
     4621 gcgtggcgcg gtcaccagcg gtgctccggg tcgtgacgga tgggcgagag gaacgggttg
     4681 agcgccagct cgcgaccgag cgacgtgtcg ggaccgtggc cgcagtgcac ggtggcgtcc
     4741 ggcgggaacc ggcggatgag cgcggccacc gagttcagca gctggtccat gtccccgccc
     4801 ggaaggtcgg tgcggccgac gctgctctgg aacaggaggt cgccgacgaa cagctcctcc
     4861 tcgccgatgg cgaacgtgta cgagccgcgc gagtgccccg gcgtcgggat ggcgagcacc
     4921 gtgatgggca gctcgagcgt ctgctcgccc tcgatggtgc gcgccgtctc gacaggctcg
     4981 atgtcgaagc cggccatgga cccgaggccg ccgtcggcca tctggccggc gtcctcggcg
     5041 cccacgtaga cggggatgtc gaagtgcctg gcgacgggcg tgaccccgcc gaagtggtcg
     5101 aagtggccgt gcgtgacgag cacggccgcc ggcgcgaaac cgaactcggt gaacgcctcg
     5161 atgacgacgt tggcctcgtc gccggggtcg atgaccagcc cctgctcccc ctgcacgacg
     5221 atgaagcagt tggcctggag cgggccgagc ggcacggcga agacttcgac gggttcaggc
     5281 attcttacct cgagtggtcg gggtggagcg gtgtgcctgc gggcgccccg ctcgttgatt
     5341 ccactgcgta gatcaatcgg cgagacagcg tatcagacgg cggcgaggac ggtcgaccgc
     5401 gccgccggcc ggcaacggcc ctacccgccg acgacccgat aggcgtcgta gacggtctgg
     5461 atgctgcgga tgttggcgag gatgttgtcg agctggcgca cgtcgccgac ctcgagtgtg
     5521 aatcggtccc gtactacgcc atcgggcagc gtctggacgc tgccgccgat gatgttcacc
     5581 cccgaatccg acagcgtgcg cgcgacgtcc tcgagcaggt ggttgcggtc gagcgcctcg
     5641 atcatgatct cgacccggaa cgccatgcct cccaggccct tccagcccac cttggtgaag
     5701 cgctcggggt tcttcatgag ggcacgggcg ttcttgcagt ccttgcggtg gatggtgatg
     5761 cccttgccga gcgagatgta gccgacgatc tcgtcccccg ggatgggctt acagcacttg
     5821 gccatgcgca ccatgatgtc gctcatgccc tcgacggcga tgccgaactc gcccgagagc
     5881 gcctgcggct cctcggcgtc ctggacgggc cgcgacggca gcatctccgg cggcggcgtg
     5941 gcctccttca tggtgcccgc gcgctgcatg accttgttgg cgacggtctt gaccggcagc
     6001 cggcccgagc ccagcgccac gtacaggtcg tcgggcttgc tgtagccgac gtccttgcag
     6061 atctgggcga acaccttgga gctcaggatc ttctgcgccg gcaggccctc gcggcgcagc
     6121 gcctcctgga gcaggtcgcg gccggactgc tccgagtcct cgcgctgctc acggcggaag
     6181 tgctggcgga tcttctggcg cgcccgcggc gagttgacga tgtcgagcca gtcgcgcgac
     6241 ggaccgcggc tcgacctgct ggtgatgacc tcgacgatgt cgccgctgct gagcttcgtg
     6301 tgcagcggca cgatgcgccc gttgaccttg gcgccgacgg tgtgattgcc gacgtcggtg
     6361 tgcacggcga aggcgaagtc gatcggcgtg ctgcccgcgg acaggctctt gacgtcgccc
     6421 ttgggcgtga acacgaacac ctcgtcctcg aagaggtcca cgcgcagggt gtccatgaac
     6481 tcgcgcgggt cgcccgtctc cgactgccac tccatcatct ggtgcagcca ggcgagct
//
LOCUS       DASUMX010000208         3507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1944120, whole genome shotgun sequence.
ACCESSION   DASUMX010000208 DASUMX010000000
VERSION     DASUMX010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3507
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1944120"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2053
                     /locus_tag="VFZ86_10075"
     CDS             <1..2053
                     /locus_tag="VFZ86_10075"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX5642665.1"
                     /translation="KAAEAAARQAAGQAAALVSADRRTTILPVTMAVPSGEVDEHMAR
                     LYEIVSGADGQSGFTVVLTGTGAWERDASEIAESDLRRGEAIGVPIALLILLIVFASL
                     VAALVPLALGVVAIIVGAALTALLGEGFMMSVFALNVVMMMGLAVGIDYTLFILSRFR
                     EELAAGRAVPDAVGRSAATASRAVLFSGMTVIIALCGMLVVPFTIFTSLGAGAILVVA
                     AAVAAATTLLPAVLGLLGRRVNALRLPLGRLGRRAHTADASVSPAAQPGSSPAPSGGF
                     WARTAAAIMRRPVVSLAAGAGVLLLLASPGLDMQRGESGITGLPQEIGTRRGYEILAA
                     DFSAGLTSPILVAVDGDLQDPRVAVALEKLRAAAQADGRFSVTAFESSDDGDFGVLSL
                     AVDADGTGAEAARAVRELRSDIIPAATAAAPLRVLVGGGPAQFADMIDLIDLYTPIVI
                     AVVLSLSCLLLLVAFRSLVIAIKAVFMNLLSVGAAYGLLTLVFQEGAGASLFGFQQTG
                     VVESWVPLLLFCILFGLSMDYHVFLLSRIRERYDQCGDTREAVSSGLQSTAGIITGAA
                     LIMVAVFAGLAAGEMVMFQQIGFGLAVAVAIDATLVRIVVVPSTMALLGRWNWYLPRW
                     LQWLPHVSLDERVPEDGLADVSVPSPSAGDPAAGAPGATPPRPRPSAARTPAPNPARR
                     P"
     gene            complement(2077..2247)
                     /locus_tag="VFZ86_10080"
     CDS             complement(2077..2247)
                     /locus_tag="VFZ86_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642666.1"
                     /translation="MNARVELACTVCTEEFVVPKAVYEAHSGVVLCPCCGSTDLLLLG
                     EVQWTGQRSLAL"
     gene            complement(2351..2566)
                     /locus_tag="VFZ86_10085"
     CDS             complement(2351..2566)
                     /locus_tag="VFZ86_10085"
                     /inference="COORDINATES: protein motif:HMM:NF018096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1059 domain-containing protein"
                     /protein_id="HEX5642667.1"
                     /translation="MYEVSCRDLGVADCEFDVMAHSLERLERAVLTHARYSHPDACAA
                     LDADPDSPERRALRARILAAAREVAVV"
     gene            complement(2814..2984)
                     /locus_tag="VFZ86_10090"
     CDS             complement(2814..2984)
                     /locus_tag="VFZ86_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642668.1"
                     /translation="MRIHRELRSARTSALTTEERHQALRAAEKSEPKGDRPTPGTGEA
                     RGATPGSRAGSP"
     gene            3098..3382
                     /locus_tag="VFZ86_10095"
     CDS             3098..3382
                     /locus_tag="VFZ86_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642669.1"
                     /translation="MGCCLIVGILAASPRLILLGMWIFSDYLTRAGLPFIWGLIGFLF
                     APSTTIAYSIAENSFGGLQGWGTVIFAVGIILDILIYYSGRKGRRERRST"
BASE COUNT          407 a         1239 c         1286 g          575 t
ORIGIN      
        1 caaggccgcc gaggcggccg cccggcaggc ggccggtcag gcggcggccc tggtctccgc
       61 cgaccgccgc acgaccatcc tgccggtgac gatggcggtg ccgtcgggcg aagtggacga
      121 gcacatggct cgcctgtacg agattgtcag tggcgcggac ggacagagcg gcttcaccgt
      181 ggtcctgacc ggcaccggag cgtgggaacg cgacgcttcc gagatcgccg agagcgacct
      241 gcgccgcgga gaggcgatcg gtgtcccgat cgcgctgttg atcctcctca tcgtgttcgc
      301 ctccctggtc gcggctctcg tgccgctggc gctcggcgtg gtggcgatca tcgtcggggc
      361 ggcgctcacg gcgctcttgg gcgaggggtt catgatgtcg gtgttcgctc tcaacgtcgt
      421 gatgatgatg ggcctggccg tgggtatcga ctacacgctg ttcatcctct cccgcttccg
      481 cgaagagctg gcggcgggcc gcgcggtgcc cgatgccgtc gggcggtccg cggccacggc
      541 cagccgcgcc gtgctcttca gcggcatgac cgtgatcatc gcgctgtgcg gcatgctcgt
      601 cgtgccgttc acgatcttca ccagcctcgg cgccggcgcg atcctggtcg tcgccgcagc
      661 ggtcgccgcg gcgacgacgc tgctgcctgc cgtgctcggc ctgctgggcc gccgcgtgaa
      721 cgcgctgcga ctgccgctgg gccggctggg gcggcgcgcc cacacggctg atgcgtccgt
      781 gtcgccggcc gcgcagccgg gctcgtcccc ggcgccgtcc ggagggttct gggcgcggac
      841 cgccgccgcc atcatgcgac gcccggtcgt gagcctggcg gccggggccg gcgtcctcct
      901 gctcctcgcc tcgcccggtc tggacatgca gcgcggcgag tcgggcatca ccggcctgcc
      961 ccaggagatc ggcacccggc gtgggtacga gatcctcgcc gcggacttct ccgcggggct
     1021 cacgtcaccc atcctggtgg ccgtcgacgg cgacctgcag gacccgcggg tcgccgtggc
     1081 gctggagaag ctgcgtgcgg cggcgcaggc cgacggccgc ttctccgtca ctgccttcga
     1141 gagcagtgac gacggcgact tcggcgtgct cagtctcgcc gtcgacgccg acggcaccgg
     1201 cgctgaggcg gcccgggcgg tgcgcgaact gcgcagcgac atcatccccg ccgcgactgc
     1261 cgccgccccc ttgcgcgtcc tcgtcggcgg cggccctgcc cagttcgccg acatgatcga
     1321 cctcatcgat ctctacacac ccatcgtcat cgccgtcgtg ctgtcgctca gttgcctcct
     1381 gctgctcgtc gcgttccgct cgctggtgat cgcgatcaag gcggtcttca tgaacctgct
     1441 cagcgtcggg gccgcgtacg gtctgctgac gctggtgttc caggagggcg ccggcgccag
     1501 tctgttcggg ttccagcaga ccggcgtggt cgagtcttgg gtgccgctgc tcctcttctg
     1561 catcctgttc ggcctctcca tggactacca cgtgttcctg ttgagccgga tccgcgagcg
     1621 ctacgaccag tgcggcgaca cccgggaggc cgtctcttcc gggctgcagt ccacagcggg
     1681 aatcatcacc ggagcggcgc tcatcatggt cgccgtgttc gcggggctcg ccgcgggcga
     1741 gatggtcatg ttccagcaga tcggtttcgg gctggccgtg gccgtggcga tcgacgcgac
     1801 cctggtgcgc atcgtggtcg tcccgtcgac catggcgctg ctcgggcgct ggaactggta
     1861 cctgccccgg tggctgcagt ggctgccgca cgtgagcctg gacgagcggg ttccggaaga
     1921 cgggctcgcg gatgtctccg tgccttcccc gtcggccggc gacccggccg cgggcgcgcc
     1981 gggcgctacg ccgccgcgcc cgcggccaag cgccgcgcgg actcccgcgc cgaacccggc
     2041 gcgtcgtccg tagaaggact cctcacgagt ggtggctcag agggcgagcg accgttgccc
     2101 cgtccactgc acctcgccga gcagcagcag gtcggtgctg ccgcaacacg ggcacagcac
     2161 cacgccggag tgtgcctcgt acacagcctt gggtaccacg aactcctcgg tgcacaccgt
     2221 gcacgcgagt tcgacgcgcg cgttcatctc gtctcctcat cgtcgtgtgc cgtgcggact
     2281 ttgtacgcga cgggcgagaa ggcggcgtga ggcgtgtgtg tgcgggcgat gaaggcgcgg
     2341 cccgcggcgc tcagacgacg gcgacctcgc gggcggcggc gaggatccgc gccctgaggg
     2401 cgcgacgctc cggcgagtcc ggatccgcgt ccagcgccgc gcaggcgtct ggatgggagt
     2461 agcgcgcgtg cgtcaggacg gcgcgctcca ggcgttcgag ggagtgggcc atcacgtcga
     2521 actcgcagtc cgcgacaccc aggtcgcggc acgacacctc gtacatggtc cagcctcctc
     2581 tcaccggcag cgacgatgac gccggtagtg cgcacctcca gtattccctg ccggagggcc
     2641 cgatgcggga gcgggaacgc gaatggggtg cgcgccgggc cccttctggg gtgcgcaccg
     2701 gatgccggca gtgcgggagg gggctccctg ccggcctcgg cggccggccc ggccgcgagc
     2761 gtgccgtgcc gggcctcagg gccggggggc gagaaagagg cctgccgcgg cgctcagggg
     2821 ctgccggcgc gcgagccggg ggtggcgccg cgcgcttcgc cggtccccgg cgtgggccgg
     2881 tcgcccttgg gctccgactt ctcggccgcc cggagcgcct gatggcgctc ttccgtggtc
     2941 aaggccgagg tcctggcgga gcgcaactcc cggtggatcc tcatgcggtg agtcatcatg
     3001 acgaccagag tgtgcccggc gcgtgttagc gttgcgtgag ttgtggctga agaggcgacg
     3061 acccgttccg gagcgcgggc gggtgagggg agacggcatg ggctgttgtc tgatcgtcgg
     3121 gatcctggcg gcgtcgccgc ggctcatcct cttggggatg tggatcttct ccgactacct
     3181 gacgcgcgcc gggctcccgt tcatctgggg cctcatcggg ttcctcttcg cgcccagcac
     3241 caccatcgcc tactcgatcg ctgagaactc cttcggcggc ctgcaaggct gggggacggt
     3301 gatcttcgcg gtggggatca tcctcgacat cctcatctac tacagcggcc gcaagggccg
     3361 gcgggagcgg cgctcgacct gagcccgcag gggccggcgt ctcgtccgcc gtgcgcgggg
     3421 ccctgcgggc cgcacgtcgc tcaacgtcac tacggcgcct gcggcgcctg cgagcgtcag
     3481 tccctgggcg gggctgcggc cggcggc
//
LOCUS       DASUMX010000209         2514 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1655812, whole genome shotgun sequence.
ACCESSION   DASUMX010000209 DASUMX010000000
VERSION     DASUMX010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1655812"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..1509
                     /gene="mtbB"
                     /locus_tag="VFZ86_10100"
                     /pseudo
     CDS             121..1509
                     /gene="mtbB"
                     /locus_tag="VFZ86_10100"
                     /inference="COORDINATES: protein motif:HMM:NF021053.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="[dimethylamine--corrinoid protein]
                     Co-methyltransferase"
     gene            1517..>2514
                     /locus_tag="VFZ86_10105"
     CDS             1517..>2514
                     /locus_tag="VFZ86_10105"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HEX5642670.1"
                     /translation="MASQLDLSRRQPLLPLGSDFAVRFYDAAKLDRYEDAVLGVLERT
                     GVRFGSPRALDILAEHGAQVDRATGVVRFAPDLVTAALAKAPRTFWLGSRDGTLDLDL
                     ASGETYNTTNGCGTEVVDWHTGVRRPPTKDDLAAITRMVDYLGSVQFWWPNVAAADKG
                     DTHTLHELDAAWNNTAKHLQGMVQGEREARYAVEMATVVAGGAEQLRRRPPMSNLIGT
                     VQPLVNDKDGIEAALVFAEAGIPVCFVTMPTYGTTAPATKAGAYVMGFAELVSATVCV
                     QLAHPGAPVILFPLPVHADPRTAALTTAPLNHRGLFLPGELAHHFGLPAMTGYAGT"
BASE COUNT          385 a          934 c          860 g          335 t
ORIGIN      
        1 cgcccggagc gccgtcttga cacccggccg accgcgccga tatcgtccag agagctttcg
       61 gttagttata cgactaacta acgaggatcg ccccgcggtc gggggacgac gggaggatct
      121 atggcgcagc gcatcccgac gaggcttggc gacggctcgc tcgtcgagct gaccagggga
      181 cagatcgagg cggacctgag cgccggctcg gaggcggcgg tcaagcgggc cagggtcgag
      241 ccgctcacgc aggacgagct cgaccacctg ctcgacatct tcacctcgcc ggccaagttc
      301 agctccgtcg attacgggga cgaggtgatc ctcggcttcg acgggtcggg caacgccgac
      361 atcggcgccc cggtgctgga gcaagtcgtc taccagaacc accacggcgc cgacatgatc
      421 gagctctggc accacgacta ctcgtacaag gccgtgcgca ccgtcctctc gttccagacg
      481 caattgctca aggacgccca gcagcagacg gtcatcccgc tcaactacgg cgccatgccc
      541 gatctgggcc gctactcaca gccggacggc ccctgcccca actggtcgga gctgatgccg
      601 ctcggcagga tcgcggaggc gagggcggcc caggaagagg cggtcgagca cgccgagcgc
      661 gacatgtggt gcgtcgccga ggcgatgatc gcggcgggcg ccgacgggct cgacttcgac
      721 accgccggcg cctccggcga cgccgacctg ctggccacgc tgcgcacggc gcgccgcctg
      781 cgcgagacct accccgacat cggtgtcgag atcggccagg cggccgagct ggtgctcggc
      841 atgcacggcg agctcgagtt cgactgcgtc cggctggccg gccagtggcc gctcggccag
      901 ctccgcgcct gcaccgccgc gggcgccacg atcttcgggc cggcggtgaa cgtgaacacc
      961 acgaagtcgc tggcgtggaa caccgcccgc gcctgcgcgc tggtcaagcc tgtgaccgcc
     1021 gaagccacca tccccgtgca catgaacgcg gggatgggcg tcggcggcgt ccccatgacc
     1081 ccgtaccctc ccggcgacgc cacctcccgc gcctccaggg cgctcgtcga catcctcaag
     1141 ctcgacgggt tgtaggtcgg catcggagac cccctcggga tggcctcctc tcacgcgctc
     1201 gcctcgggca tgggcgggat gcggaccgcc ggagatctgg tggcccgcat gcagatgacc
     1261 cggggtatgc gcctcccgca agccaagcgg tacgtcgccg acaagctcgg ctgctcggtg
     1321 ctcgacctca gcgacaccgt ggcgatgcac gacatccgcc gcgacctcgg cctcggccgc
     1381 atcccggtgc aggacctcac ctacgccgac cacccgggcg cgatggaggc caagttccgc
     1441 atcgccgagg tgctcggcgt cccgatcaac tcggtgcagc gcttcaaggc gcacgccggg
     1501 ctggcctgac gggcccatgg cctcgcagct cgacctcagt cgtcgccagc cgctgctgcc
     1561 gctcggctcg gacttcgcgg tgcgcttcta cgacgcggcg aagctggaca ggtacgagga
     1621 cgccgtgctc ggcgtgctgg agcggaccgg cgtgcgcttc ggctcgccca gggcgctcga
     1681 catcctggcg gagcacggcg ctcaggtgga tcgcgcgacc ggcgtcgtgc gcttcgcgcc
     1741 cgacctcgtg acggccgcgc tggccaaggc cccgcggacg ttctggctcg ggtcgcgcga
     1801 cggcacgctc gacctcgacc tcgcgagcgg cgagacctac aacaccacca acggctgcgg
     1861 caccgaggtc gtcgactggc acaccggcgt gcgccggccg ccgaccaagg acgacctcgc
     1921 cgccatcacg cgcatggtgg actacctggg ctcggtccag ttctggtggc ccaacgtggc
     1981 cgcggccgac aagggcgaca cgcacacgct gcacgagctg gacgccgcct ggaacaacac
     2041 cgccaagcac ctccagggca tggtgcaggg cgagcgcgag gcgcggtacg ccgtggagat
     2101 ggcgaccgtg gtcgcgggag gggcggagca gctgcgccgc cgccccccca tgagcaacct
     2161 catcggcacc gtgcagccgc tggtcaacga caaggacggc atcgaggcgg cgctcgtgtt
     2221 cgccgaggcc ggcatccccg tgtgcttcgt cacgatgccg acctacggga ccacggcgcc
     2281 ggccaccaag gccggcgcct acgtgatggg gttcgccgag ctggtcagcg ccaccgtctg
     2341 cgtgcagctg gcgcaccccg gcgcgccggt gatcctcttc cctctgccgg tgcacgccga
     2401 cccacggacg gcggcgctga ccacggcgcc gctcaaccac cgcggcctct tcctccccgg
     2461 cgagctggcg caccacttcg ggctgccggc gatgacgggg tacgccggca ccga
//
LOCUS       DASUMX010000210         2485 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1369067, whole genome shotgun sequence.
ACCESSION   DASUMX010000210 DASUMX010000000
VERSION     DASUMX010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1369067"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="VFZ86_10110"
     CDS             <1..630
                     /locus_tag="VFZ86_10110"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX5642671.1"
                     /translation="AHLDGITYTTYKDMGRMGLSTGGWIYKDVELPRKYVLGEEGKGF
                     YVNMDGFNAARVLVAAACLGGAEKALEISGEYTAQRTTFGKPLGKWEGISFEMADDWM
                     QLDMLKLNLLRGAWMIGQEHTNPGTFGRKEINKVISTCKCLSPALCHDIARHGMMYHG
                     AFGYTKETPLEMLLRGVMSYEVGAEGGLMIMRGIIARDEWGETHNPFKD"
     gene            668..1957
                     /locus_tag="VFZ86_10115"
     CDS             668..1957
                     /locus_tag="VFZ86_10115"
                     /inference="COORDINATES: protein motif:HMM:NF015451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5642672.1"
                     /translation="MESVECIVVGGGLAGLSAAYGLAEAGLEVMVLERGDYSGAKNVT
                     GGRLYVKPIRGFYPELWDEAPFERPVARELVTMMGPGAHTTVEIASDRFTEGEPQSYT
                     VVRARLDQWLAEKVMEKGAMVVPNMKVDELLHKDGRVIGIRAGGDEIGASVVIVAEGV
                     LRLLSSAAGLAAEPQARHHALGYKEIIELPAGVIEDRWRLNPGEGAAQLFMGECTRGM
                     MGGAFLYTNKESISLGMVVGMEDMRRRQDGTESWQLLDAFKELPQIRPLVAGGAVAEY
                     SAHAVPEGGISQVPRLAGDGYLVAGDTAGLSLNALVTVRGMDLAIASGYFAAEAVKKA
                     KAAGDFSAAGLASYEASLRESFVLKDLETARAIPGFMENPRLFTHYPKAVSGLLESVY
                     TVGERPQAGLFKKVWKSARKDFMNVATVKDAWSMRKI"
     gene            1970..2251
                     /locus_tag="VFZ86_10120"
     CDS             1970..2251
                     /locus_tag="VFZ86_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015945339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX5642673.1"
                     /translation="MPMKIDAKLGLDVFKLDKEPHIVIDTDVCTAVCTDEACIYVCPA
                     DLYEKDDQGRMTVNWEGCLECGTCMICCDHRALSWTYPSGPFGVQYRMS"
     gene            2265..>2485
                     /locus_tag="VFZ86_10125"
     CDS             2265..>2485
                     /locus_tag="VFZ86_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein beta subunit/FixA
                     family protein"
                     /protein_id="HEX5642674.1"
                     /translation="MDIVVCVKTNPDLQMVRIKNREAVTEAVPYKIGDLEKNALQAAV
                     DLRDAVGGKVVALCLAEGNRKAAEDIKVAL"
BASE COUNT          444 a          821 c          878 g          342 t
ORIGIN      
        1 gcgcacctcg acggcatcac ctacacgacg tacaaggaca tgggccgcat gggcctcagc
       61 accggcggtt ggatctacaa ggacgtcgag ctgcccagga agtacgtgct cggcgaagag
      121 ggcaagggct tctacgtgaa catggacggc ttcaacgccg cccgcgtgct cgtggcggcc
      181 gcgtgcctcg gcggcgcgga gaaggcgctc gagatcagcg gcgagtacac ggcccagcgc
      241 accacgttcg gcaagccgct cggcaagtgg gagggcatct ccttcgagat ggccgacgac
      301 tggatgcagc tcgacatgct caagctcaac ctgctgcgcg gcgcgtggat gatcggccag
      361 gagcacacca acccgggcac gttcgggcgc aaggagatca acaaggtcat cagcacctgc
      421 aagtgcctga gccccgcgct ctgccacgac atcgcccgcc acgggatgat gtaccacggc
      481 gccttcggct acaccaagga gacgcccctc gagatgctgc tccgcggcgt catgagctac
      541 gaggtcggcg ccgagggcgg cctgatgatc atgcgcggca tcatcgcccg cgacgagtgg
      601 ggcgagaccc acaacccgtt caaagattga cggtactgac ctgacgaggc cggagggagg
      661 ccgccacatg gaatcagtcg agtgcatcgt cgtgggtggc ggcctcgccg gcctctccgc
      721 ggcctacggc ctggccgagg ccgggctcga ggtcatggtc ctcgagcgcg gggactactc
      781 gggcgccaag aacgtcaccg gcgggcgcct gtacgtgaag cccatccgcg gcttctaccc
      841 ggagctgtgg gacgaggcgc ccttcgagcg gccggtggcg cgcgagctgg tcacgatgat
      901 gggccccggc gcgcacacca cggtcgagat cgcctcggac aggttcaccg agggtgagcc
      961 gcagagctac accgtcgtgc gcgccaggct cgaccagtgg ctcgccgaga aggtgatgga
     1021 gaagggcgcg atggtcgtgc ccaacatgaa ggtcgatgag ctgttgcaca aggacggccg
     1081 cgtgatcggc atccgcgccg gcggcgacga gatcggcgcg agcgtcgtca tcgtcgccga
     1141 gggcgtcctg cggctgctca gcagcgcggc cgggctggcg gcagagccgc aggcgaggca
     1201 ccacgcgctc ggctacaagg agatcatcga gctgccggcg ggcgtgatcg aggaccgctg
     1261 gcgcctcaac cccggcgagg gcgccgccca gctcttcatg ggtgagtgca ccaggggcat
     1321 gatgggcggc gcgttcctct acaccaacaa ggagagcatc tccctgggca tggtggtggg
     1381 catggaggac atgcgccgcc ggcaggacgg cacggagagc tggcagctcc tcgacgcgtt
     1441 caaggagctg ccgcagatcc ggccgctcgt cgccgggggc gcggtcgccg agtactcggc
     1501 gcacgccgtg cccgagggcg gcatcagcca ggtgcccagg ctcgccggcg acggctacct
     1561 ggtggccggc gacaccgcgg ggctcagcct caacgccctg gtcaccgtgc gcggcatgga
     1621 tctcgccatc gccagcggct acttcgccgc cgaggcggtc aagaaggcga aggcggccgg
     1681 cgacttctcg gccgccggcc tcgcctccta cgaggcgtcc ctgcgcgaga gcttcgtgct
     1741 caaggacctc gagacggcgc gggcgatccc cggcttcatg gagaacccgc gcctgttcac
     1801 gcactacccc aaggccgtct cggggctgct cgagagcgtc tacaccgtcg gcgagcggcc
     1861 ccaggccggc ctcttcaaga aggtctggaa gagcgccagg aaggacttca tgaacgtggc
     1921 gaccgtcaag gacgcctggt cgatgcggaa gatctgagag gagggcgaca tgccgatgaa
     1981 gatcgacgcc aagctcggcc tcgacgtctt caagctcgac aaggagccgc acatcgtcat
     2041 cgacaccgac gtgtgcacgg cggtctgcac ggacgaggcc tgcatctacg tgtgccctgc
     2101 cgacctctac gagaaggacg accagggccg catgaccgtg aactgggagg gctgcctcga
     2161 gtgcggcacc tgcatgatct gctgcgatca ccgcgccctc tcgtggacgt acccgagcgg
     2221 ccccttcggg gtccagtacc ggatgagctg aaggaggtga cggcgtggac atcgtcgtct
     2281 gcgtgaagac caatccggac ctccagatgg tccgcatcaa gaaccgcgag gcggtcaccg
     2341 aggccgtgcc gtacaagatc ggcgacctcg agaagaacgc cctccaggcg gcggtggacc
     2401 tgcgcgacgc cgtgggcggc aaggtggtcg ccctgtgcct cgccgagggc aaccgcaagg
     2461 ccgccgagga catcaaggtc gcgct
//
LOCUS       DASUMX010000211         2674 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_938554, whole genome shotgun sequence.
ACCESSION   DASUMX010000211 DASUMX010000000
VERSION     DASUMX010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_938554"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..837)
                     /locus_tag="VFZ86_10130"
     CDS             complement(<1..837)
                     /locus_tag="VFZ86_10130"
                     /inference="COORDINATES: protein motif:HMM:NF012907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HEX5642675.1"
                     /translation="MPVDADSTRRRARRPRRRLPALVVIVAVGFVAVAAALALATALD
                     DDRPHGPYLIGAWTFGDRGSLERAVDAGALDEVSADWLQSRADGTVVAPRLDTGFIEA
                     ARDRDCRVFVTLTDYDQAEHTFDPSISAAILRTAATRRRHAAAVAAWCREYDVAGVDV
                     DWEAVKGSRRDAFSAFVEELAARLHADDRLIAVDVYPKLREPGGWDGPRGQDWERLGR
                     AVDQFRVMTYNYSGSWSGPGPLSPPEWMDRVIGFAETQVAPRRIVMGLGFYGREWRGS
                     ATA"
     gene            974..1534
                     /locus_tag="VFZ86_10135"
     CDS             974..1534
                     /locus_tag="VFZ86_10135"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014261535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEX5642676.1"
                     /translation="MKEKLGATNALYPLVTAIVGATVDGTPDFATIAHVGIAHLKGIT
                     LGMGNIHLTNKGIIENETFSVNLPSEELVALTDHIGMVSGTQEDKSGLFELFYGELDT
                     APMIQECKVTMECKLIDHVKLPTHDLFVGEVVGTYADLDVLTNGIVDIAKLKPLLFDM
                     ASKKYWGLGDPVAKCWSVGKEWKPAS"
     gene            complement(1568..2086)
                     /locus_tag="VFZ86_10140"
     CDS             complement(1568..2086)
                     /locus_tag="VFZ86_10140"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEX5642677.1"
                     /translation="MSIEYVRQQLGDAERILYTERHHWVFFVAEMLKWILFAAAVLAL
                     IVLLRVWWLPDAGWVWWLLLVFIVPAARIAWGFLSWRMNVYVLTSRRVIESTGVLSKR
                     VADSSLEKLTDIVLRQSVLGRMLHYGDIVVLTASSGAGINNLKQIRRPMEFKTEMANA
                     KEALDREMGASD"
     gene            2170..>2674
                     /locus_tag="VFZ86_10145"
     CDS             2170..>2674
                     /locus_tag="VFZ86_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642678.1"
                     /translation="MRLRPSVLVLSLVVCAVALGAAAALPQAAAASAPVRSYRGLGAW
                     VDIYDAKAWDHPAAAVRDMAARGVRTLYLETANYHLPADSTMFRRADAGRLIEEAHAR
                     GIKVVAWYLPGFKDPARDYRRSVAAIRYRTPSGRRFDSFALDIEASIVKDVALRNSRL
                     RALSRRIR"
BASE COUNT          425 a          963 c          915 g          371 t
ORIGIN      
        1 ggccgtcgcg gagccgcgcc actcgcggcc gtagaagccg agacccatga cgatgcgccg
       61 tggggccacc tgcgtctccg cgaagccgat cacccggtcc atccactccg gcggggagag
      121 cgggccgggg ccggaccacg acccggagta gttgtaggtc atcacgcgga actggtcgac
      181 cgcgcggccg agccgctccc agtcctgccc ccgcgggccg tcccagccgc ccggctcgcg
      241 cagcttggga tagacgtcca cggcgatcag gcggtcgtcc gcgtgcaggc gtgcggcgag
      301 ctcctccacg aacgccgaga acgcgtcacg gcgcgaaccc ttgaccgcct cccagtccac
      361 gtcgacgccg gcgacgtcgt actcgcgaca ccaggcggcg acggccgcgg cgtgccgccg
      421 tcgcgtcgcc gccgtcctga ggatcgccgc cgagatcgag gggtcgaagg tgtgctccgc
      481 ctggtcgtag tcggtcagcg tgacgaacac gcggcagtcc ctgtcccgcg ccgcctcgat
      541 gaaccccgtg tcgagacgcg gcgccaccac cgtgccgtcc gcccgggact gcagccagtc
      601 ggcgctgacc tcgtcgagtg cgcccgcgtc caccgccctc tccagcgaac cgcgatctcc
      661 gaaggtccag gcgccgatga ggtacgggcc gtgcgggcgg tcgtcgtcga gcgccgtggc
      721 cagcgcgagg gccgcggcga cggcgacgaa ccccacggcg acgatgacga ccagggccgg
      781 caggcgccgc cggggccggc gagcgcggcg ccgcgtgctg tcggcgtcga cgggcatgcg
      841 cccatgatga cggctgcgcg cgcggcgcga aaccggcgga gcccagggct gcgaggctcc
      901 ctcgattacg ctcgcccccc gggcgggtat gcagcggtca cacaccgcac atcccgcgac
      961 cagtgaggtc accgtgaagg agaagctcgg cgccaccaac gcactctacc cgctcgtcac
     1021 cgccatcgtc ggcgccaccg tggacggcac acccgacttc gcgaccatcg cccacgtcgg
     1081 catcgcccat ctcaaaggca tcaccctcgg catgggcaac atccacctga ccaacaaggg
     1141 catcatcgag aacgagacgt tcagcgtgaa cctgccctcg gaggagctgg tcgcgctcac
     1201 cgatcacatc ggtatggtga gcggcaccca ggaggacaag agcggtctct tcgagctctt
     1261 ctacggggag ctggacacgg cgccgatgat ccaggagtgc aaggtgacga tggagtgcaa
     1321 gctcatcgac cacgtcaagc tgccgaccca cgacctcttc gtgggcgagg tcgtgggcac
     1381 ttatgcggat ctggatgtgc tcaccaacgg gatcgtcgac atcgccaagc tcaagccgct
     1441 gctcttcgac atggcgagca agaagtactg gggtctcggc gacccggtgg ccaagtgctg
     1501 gagcgtcggc aaggagtgga agccggcgag ctgaggcgcc tcgggcggtg cgcggggggc
     1561 gggcgcgtca gtcgctcgcg cccatctccc gatcgagcgc ttccttggcg ttggccatct
     1621 cggtcttgaa ctccatgggc cggcggatct gcttcaggtt gttgatgccg gcgccggagg
     1681 aagccgtgag cacgacgatg tcgccgtagt gcagcatgcg gccgaggacg gactgcctga
     1741 gcacgatgtc ggtgagcttc tcgagcgacg agtcggcgac gcgcttgctc agcacgcccg
     1801 tggactcgat gaccctgcgg ctggtgagca cgtacacgtt catgcgccag gagaggaagc
     1861 cccaggcgat gcgcgccgcc ggcacgatga agacgaggag cagccaccac acccagccgg
     1921 cgtccggcag ccaccacacc ctgagcagga cgatcagcgc gaggacggcc gccgcgaaga
     1981 ggatccactt gagcatctcg gcgacgaaga acacccagtg gtgccgctcg gtgtagagga
     2041 tcctctccgc gtcgccgagc tgctgccgca cgtactcgat gctcacatgc cctcctcgcg
     2101 ccccggtgtc gggtgcaccc taccacggct atactgagcc ccaataccgg ccaccgcagg
     2161 ggtcttctcg tgcgccttcg tccttccgtc ctcgtcctgt cgctcgtcgt ctgcgcggtc
     2221 gcgctggggg cggccgcggc gctgccgcag gcggccgccg cctcggcgcc cgtccgcagc
     2281 tatcgcggtc tcggcgcgtg ggtcgacatc tacgacgcca aggcctggga ccacccggcg
     2341 gcggcagtca gggacatggc ggcgcgcggc gtccgcacgc tctacctgga gacggccaac
     2401 taccacctgc ccgcggactc gaccatgttc cggcgcgccg acgccggcag gctgatcgag
     2461 gaggcgcacg cacgcggcat caaggtggtg gcctggtatc ttcccggctt caaggacccc
     2521 gccagggact acaggcgcag cgtggcggcg atccgctacc gcacgccgtc cggccggcgc
     2581 ttcgactcgt tcgcgctcga catcgaggcg agcatcgtca aggacgtcgc cctgcgcaac
     2641 agccggctgc gggcgctctc gaggcgcatc cgcg
//
LOCUS       DASUMX010000212         9938 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1513532, whole genome shotgun sequence.
ACCESSION   DASUMX010000212 DASUMX010000000
VERSION     DASUMX010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 9938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9938
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1513532"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1368
                     /gene="ftsH"
                     /locus_tag="VFZ86_10150"
     CDS             <1..1368
                     /gene="ftsH"
                     /locus_tag="VFZ86_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002563101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEX5642679.1"
                     /translation="VLLYGPPGTGKTLLARAVAGEAGVPFFSISGSDFVEMFVGVGAS
                     RVRDLFDQAKQNAPCIIFVDEIDAVGRHRGAGMGGGHDEREQTLNQLLVEMDGFEMKD
                     NIILIAATNRPDILDPALLRPGRFDRQIVVDRPDRPGRKQILDVHSRGKPIDKDIDID
                     ALAAQTPGFTGADLANLVNEAALLAARHGKKLIGMTELEEGIMRVLAGPEKKTRIISE
                     KEKAITAYHEMGHALVGHFLEFTDPVHKISVVSRGQALGYTISLPSEDKFLTTKQELM
                     DTLSMTLGGRAAEEVVFGEITTGAANDLEKATATAKQMIMRYGMSEELGPRTLGHDQS
                     MPFLGREFQQQADYSEEIAKQIDDEIRRIIEEAHQRAKDLLAEKRDLLDRISKILITR
                     ETIEAHEFQALVDGLPEDEVFRERDEKEAQRRAEPPEAEKRQRAKQPPVPKPAHVNPV
                     NPEAT"
     gene            1397..1966
                     /gene="folE"
                     /locus_tag="VFZ86_10155"
     CDS             1397..1966
                     /gene="folE"
                     /locus_tag="VFZ86_10155"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEX5642680.1"
                     /translation="MDSDKIKAGVRLILEGIGEDPERPGLRKTPQRVADMYADIFSGI
                     GAPDPAELLTAIPGDHHREMVLIKDIDFYSICEHHLLPFHGRAHVAYIPSDHGHIAGL
                     SKLARVVRQVAARPQLQERITSEVADAIETSLQPTGALVLIEAEHLCMSMRGVRTPGS
                     KTVTSAVRGLIQTSEATRAEVFALINAPH"
     gene            1978..2820
                     /gene="folP"
                     /locus_tag="VFZ86_10160"
     CDS             1978..2820
                     /gene="folP"
                     /locus_tag="VFZ86_10160"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEX5642681.1"
                     /translation="MLPAAPTFSLDLQFPAIMGVVNVTPDSFSDGGRFLAADAALEQA
                     LTLVREGASIVDIGGESTRPGSEVVPEDEELRRVVPVVEALAGSVGVPISVDTMKAGV
                     ARRALAAGAAIINDVSALRFDDEMVDVVIESGCPVCLMHMKGQPKTMQDDPRYDDVVD
                     EVLRFLEERLVFAQARGVREEQIMVDPGIGFGKTVAHNLALLDGLDRFVALGRPVLLG
                     ASRKRFLGAILGNEPTGRAIGTVATTVIGYLAGAHIFRVHDVKPNFEALRVALAVREG
                     VTAP"
     gene            2860..3225
                     /gene="folB"
                     /locus_tag="VFZ86_10165"
     CDS             2860..3225
                     /gene="folB"
                     /locus_tag="VFZ86_10165"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEX5642682.1"
                     /translation="MTRSLSIILEGIELRGRCGVTAEERAIGQTLVVDVRLDPQSLSS
                     GQSDDLDDTVNYSRVVDYVRGVVEGDEFHLLERLATVICDGLWDAFDPALVEVAVSKI
                     APPTKLPIHAARVEVVRTA"
     gene            3252..3731
                     /gene="folK"
                     /locus_tag="VFZ86_10170"
     CDS             3252..3731
                     /gene="folK"
                     /locus_tag="VFZ86_10170"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEX5642683.1"
                     /translation="MSLGSNLGDRAGYLRAAREALSALPATTVVAASRVYETAPQERD
                     DQDAFLNQVVCLETGLQPLDLLAECQRIESEHGRVRTLRFGPRTLDIDILLFQDVASD
                     DPGLTLPHPRMVKRAFVLVPLAEVWEQARGMPDMDVEKLGREAARDQRVRLYDATEG"
     gene            3770..5410
                     /gene="nadB"
                     /locus_tag="VFZ86_10175"
     CDS             3770..5410
                     /gene="nadB"
                     /locus_tag="VFZ86_10175"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019536391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HEX5642684.1"
                     /translation="MTRPYVASHSGEPLHRRSFDVLVLGGGIAGLTAAIGAARRWRVG
                     LLTKSTFEETTTFLAQGGIAAAIGEEDSPELHFEDTVQAGAGLCDDEAVRVLVNEGPD
                     RVRELMEICPRFDKVDGQIVLGREGAHSLPRIVHAGGDATGSEVSSALAEAARLGSRV
                     QLYEDEFVIDLLTVDGRCVGALTLNLADGGLTLNVAMVTVLATGGAGQVYGRTTNPSV
                     STGDGVAMAYRAGAAIRDLEFVQFHPTGLAVPGQQTVQLITEALRGEGAYLRNSAGQR
                     FMTEVDPRGELASRDIVVRGMVAEMRREGTHHVYLDATHLDAAWLQERFPRVTAGLRD
                     HGLDLATELIPVAPVCHYFIGGVVTDVWGRTTVPGLYASGEVASTGVHGANRLASNSL
                     LEGLVFSDRVVRDLDRYIGRLGEDVRRLSFDIPEATPDAGGSDAPAARRRLTRVLQEK
                     VGVLRRADDLQAALDELGRLTSELRFGIMGEAEYEVLNMLTLGTQIAKCALLREESRG
                     VHLRDDFPGLDDERWRTHITLRLPEHERTEGVGRVGPS"
     gene            5407..6291
                     /gene="nadC"
                     /locus_tag="VFZ86_10180"
     CDS             5407..6291
                     /gene="nadC"
                     /locus_tag="VFZ86_10180"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEX5642685.1"
                     /translation="MTSRQAGERAAGTGPLRARGYRQAARMAARRALREDLAGYGDIA
                     GSAFDGHGAARVVGREGGVLSGVAALEETAALVDPALAVRVFVEDGERFAAGDVVAEL
                     RGPLSAILAAERTALNFLCRLSGVATLTATYVAETAGTGARIAATRKTTPGLRALEKQ
                     AVVHGGGTPHRFGLFDGAMIKDNHVAAAGGVAAAVERVRAGAAHLHALEVEVDTLEQL
                     DEAIAAGAAIVLLDNMDTATVGAAVARVAGRAVVEVSGGVRLERVRELAEAGADIISV
                     GALTTRAPWIDLALDLET"
     gene            6294..7067
                     /locus_tag="VFZ86_10185"
     CDS             6294..7067
                     /locus_tag="VFZ86_10185"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018046775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEX5642686.1"
                     /translation="MLLVVDVGNTQTHLGVIDGGAVVDEWRIATVRHRTSDEIAGLLQ
                     GFFSLQSRRLREVVGEVGIASVVPRLTQQWEEMCRKYLGIPAFVVGPGARTGMRIRTR
                     NPAEVGADRIVNAVAAFEMYGGPCIVADYGTATTFDVISAEGDYLGGAIAPGIEVSLE
                     ALTSRAAKLIKVELIEPEHAIGKSTTEALQSGAVYGFAGEVEGVVHAIWAEMGEKCPV
                     IATGGLAEMIARHTDVIEKVDPFLTLRGIEIVMRRQQRD"
     gene            7087..8121
                     /locus_tag="VFZ86_10190"
     CDS             7087..8121
                     /locus_tag="VFZ86_10190"
                     /inference="COORDINATES: protein motif:HMM:NF013380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEX5642687.1"
                     /translation="MATPGPPPSGTGPARSGPAGGPCATDPLLRPLAIGGLRLERRLF
                     MAPMAGLTTSAFRRSVRRWGAGLVFTEMISAYGVHYHNRRTLDYFAHREDEHPLGFQL
                     FGADADVLADAAGRAVAAGADVVDLNMACPVRKVVKTGAGAALLAEPDRAAAIVAAVV
                     AAVEDAAPGRGVPVTVKIRSGLRDGDGLGSRAAPRLAAAGAAAVCIHPRTAAQLYRGR
                     ADHAVTFALAAALPVPVIASGDVDGREAALALLDGGAAAVMLARGAVGAPWLFAQVLG
                     EAEPSAAGKLEEVRRFAAEVLEECGGRGVGHLRQFWPGFRRHGALDKALSLQLMQARD
                     EAGLRTLLGL"
     gene            8280..8759
                     /gene="greA"
                     /locus_tag="VFZ86_10195"
     CDS             8280..8759
                     /gene="greA"
                     /locus_tag="VFZ86_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012772085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEX5642688.1"
                     /translation="MVDREIILTPEGFQRLKEEIEYLSSVKRDEVAERIRASRDFGDI
                     SENSEYDDAKNEQAMLEARIYALEERLRSAIVIDSDSITTDKVGVGTKVTLQDMQRGD
                     VVQYAIVGSAEADPGAHKLSNESPVGNAIMGHKPGDKVTVSVPQGSKKFKVLAIEKA"
     gene            8771..>9938
                     /locus_tag="VFZ86_10200"
     CDS             8771..>9938
                     /locus_tag="VFZ86_10200"
                     /inference="COORDINATES: protein motif:HMM:NF012379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HEX5642689.1"
                     /translation="MDEQHADHGDALAERRDKLTAWRGAGAAYPTRYQPRDEIARLQA
                     AYESLESGQDSPDVRRVAGRVVARRVHGKLTFVVVKDMSGELQLFCQLDALGEERYHA
                     LEHVDLGDFVGAEGTMMRTRRGELSLRITSWQLLSKSLRGMPEKYHGLTSVETRYRKR
                     YLDLAANDDSREVFIKRARIIAAIRRFLDDRGFVEVETPVLQPLYGGALARPFVTHHN
                     ELERDLYLRIATELYLKRCIIGGIDRVYEIGKDFRNEGVSFKHNPEFTMLETYEAYVD
                     YNHVMTMVEEMTAHAAEAVGSLKVTFREHDIDLTPPWPRLTMRKAIENETGIDIGADD
                     ARTVADLQARIKEKGLEDVVPLAPAWGAQVDELFSECVEPKLIQPVFITEHPLET"
BASE COUNT         1502 a         3631 c         3472 g         1333 t
ORIGIN      
        1 gtgctgctgt acggcccgcc cggcaccggc aagacgctgc tcgcgcgtgc cgtcgccggc
       61 gaggcgggcg tcccgttctt cagcatcagc ggctccgact tcgtcgagat gttcgtcggc
      121 gtcggtgcct cgcgcgtgcg cgacctgttc gaccaggcca agcagaacgc gccctgcatc
      181 atcttcgtcg acgagatcga cgccgtcggc cgccaccgcg gcgccggtat gggcggcggc
      241 cacgacgagc gcgagcagac gctcaaccag ctgctcgtcg agatggacgg cttcgagatg
      301 aaggacaaca tcatcctcat cgccgccacc aaccggccgg acatcctcga cccggcgctg
      361 ttgcgcccag gccgcttcga ccggcagatc gtggtggacc gcccggaccg gccgggccgc
      421 aagcagatcc tcgacgtgca ctcacgcggc aagccgatcg acaaggacat cgacatcgac
      481 gcgctcgccg cgcagactcc gggcttcacc ggcgccgacc tggccaacct cgtcaacgag
      541 gcggcgctcc tcgcggcccg tcacggcaag aagctcatcg gcatgaccga gctcgaagag
      601 ggcatcatgc gcgtgctcgc cggccccgag aagaagacgc gcatcatcag cgagaaagag
      661 aaggccatca ccgcctacca cgagatgggt cacgcgctcg tcggccactt cctcgagttc
      721 accgacccgg tgcacaagat ctccgtggtc agccgcggcc aggcgctcgg ctacaccatc
      781 tcgctgccca gcgaggacaa gttcctgacc accaagcagg agctcatgga cacgctgtcc
      841 atgaccctcg gcggccgcgc cgcggaagaa gtcgtcttcg gcgagatcac caccggtgcg
      901 gccaacgacc tcgagaaggc cacggccacg gccaagcaga tgatcatgcg ctacggcatg
      961 agcgaggaac tcggcccgcg gacgctcggc cacgaccagt cgatgccgtt cctcggccgc
     1021 gagttccagc agcaggccga ctactccgag gagatcgcca agcagatcga cgacgagatc
     1081 cggcgcatca tcgaggaggc gcaccagcgc gccaaggacc tgctcgccga gaaacgcgat
     1141 ctgctcgacc gcatcagcaa gatcctcatc acgcgcgaga ccatcgaggc gcacgagttc
     1201 caggcgctgg tcgacggact gcctgaggac gaggtcttcc gcgagcggga cgagaaggaa
     1261 gcccagcgcc gcgccgagcc tccggaggcc gagaagcgcc agcgcgccaa gcagccgccg
     1321 gtgcccaagc ccgcgcacgt gaacccggtc aaccccgagg ccacctgacc tgccggcgac
     1381 ggcagcaggg cgcaacgtgg actccgacaa gatcaaggcg ggtgtacgcc tcatcctcga
     1441 gggcatcggc gaggacccgg agcggccggg gctgcgcaag acgccgcagc gcgtcgccga
     1501 catgtacgcc gacatcttct ccggcatcgg cgcccccgac ccggcggaac tgctcaccgc
     1561 catccccggc gaccaccacc gtgagatggt gctcatcaag gacatcgact tctactcgat
     1621 ctgcgagcac cacctgctgc cgttccacgg gcgggcgcac gtggcctaca tccccagcga
     1681 ccacggccac atcgcggggc tgagcaagct ggcgcgcgtg gtgcgtcagg tggcggcgcg
     1741 cccgcagctc caggagcgga tcacgagcga ggtcgccgac gccatcgaga cgtccctgca
     1801 gccgaccggc gcgctcgtgc tcatcgaggc cgagcacctg tgcatgagca tgcgcggcgt
     1861 gcgcacgccg ggctccaaga cggtcaccag cgccgtgcgc gggctcatcc agaccagcga
     1921 ggccacgcgc gccgaggtgt tcgccctgat caacgcgccg cactgacgcg gccccccatg
     1981 ctgccggccg cgccgacctt ctcgctcgac ctccagttcc cggccatcat gggcgtcgtc
     2041 aacgtcacgc ccgactcctt ctccgacggc ggcaggttcc tcgccgccga cgccgccctc
     2101 gagcaggcgc tcacgctggt gcgcgaagga gcctcgatcg tcgacatcgg cggcgagtca
     2161 acgaggccgg gctccgaggt ggtgccggag gacgaggagc tgcgccgcgt ggtgccggtg
     2221 gtcgaagccc tcgccggcag cgtcggcgtg cccatctccg tcgacacgat gaaggccggg
     2281 gtcgcgcgcc gcgcgctcgc cgcgggcgcg gcgatcatca acgacgtcag cgcccttcgg
     2341 ttcgacgacg agatggtcga cgtcgtgatc gagtctggct gcccggtgtg cctgatgcac
     2401 atgaagggac agcccaagac gatgcaggac gacccgcgct acgacgacgt ggtcgacgag
     2461 gtgctgcgtt tcctcgagga acgcctggtc ttcgcccagg cgcgtggggt gcgcgaagaa
     2521 cagatcatgg tcgatcccgg catcgggttc ggcaagaccg tcgcccacaa tctcgccctg
     2581 ctcgacggcc tggaccgctt cgtcgcgctc ggtcgtccgg tcctgctggg cgcctcgcgc
     2641 aagcggttcc tcggggcgat cctcggcaac gagccgacgg gccgtgccat cggcaccgtg
     2701 gcgacgacgg tgatcggcta cctcgccggc gcgcacatct tccgcgtgca cgacgtcaag
     2761 cccaacttcg aggcgttgcg ggtcgcgctc gccgtgcgcg agggcgtcac cgccccctga
     2821 cccgaccacc ggccaccggc cggaaagagg aggcgcacga tgacgcgaag cctgtccatc
     2881 atcctcgagg ggatcgagct gcgcgggcgc tgcggggtca ccgccgagga gcgggcgatc
     2941 ggtcagacgc tggtcgtgga cgtgcgtctc gatccccagt cgctgagctc gggccagagc
     3001 gacgacctcg acgacacggt caactacagc cgcgtcgtcg actacgtgcg cggcgtggtc
     3061 gagggcgacg agttccacct cctcgagcgg ctcgccacgg tgatctgcga cgggctctgg
     3121 gacgcgttcg acccggcgct cgtggaggtc gccgtgagca agatcgcgcc gcccaccaag
     3181 ctgcccatcc acgcggcgcg cgtcgaggtc gtgcgcaccg cgtgaggtcg gctacgggca
     3241 gtcgcgtctt cgtgtcgctc ggctccaatc tcggcgaccg ggcggggtac ctgcgcgccg
     3301 cgcgcgaggc gctctcggca ctgcccgcca ccaccgtcgt cgccgcgtcg cgcgtctacg
     3361 agaccgctcc gcaggagcgc gacgaccagg acgcgttcct caaccaggtg gtctgcctgg
     3421 agaccggcct gcagccgctg gacctgctgg ccgaatgcca gcgcatcgag agcgagcacg
     3481 gtcgcgtgcg caccctgcgc tttggcccgc gcacgctcga tatcgatata ctgctcttcc
     3541 aagacgtcgc gtccgacgat cccgggctca cgctcccgca tccgcgcatg gtcaagcgag
     3601 cgttcgtgct cgttcccctc gccgaggtgt gggagcaggc gcggggcatg ccggacatgg
     3661 acgtcgagaa gctggggcgg gaggccgccc gggaccagcg ggtgaggttg tacgacgcca
     3721 cggaagggtg agcggtgagc gatgacgagc cggcgatgag gcgcgcttca tgacgcgccc
     3781 gtacgtcgcg agccactccg gggagccgct gcaccggcgg agcttcgatg tcctcgtgct
     3841 cggcggcggc atcgccggac tcaccgccgc catcggcgcc gccaggcgct ggcgcgtcgg
     3901 gctgctcacc aagtcgacct tcgaagagac caccacgttc ctcgcccagg gcggcatcgc
     3961 cgcggccatc ggcgaggagg actcgcccga gctgcacttc gaggacaccg tccaggccgg
     4021 cgccggcctc tgcgacgatg aggcggtgcg cgtcctcgtg aacgagggcc ccgaccgcgt
     4081 gcgcgagctc atggagatct gcccgcggtt cgacaaggtc gacgggcaga tcgtgctcgg
     4141 gcgggagggt gcgcactcgc tgccccgcat cgtccacgcg ggcggcgacg ccaccggcag
     4201 cgaggtctcc agcgccctcg ccgaggccgc gcgcctcggc agccgcgtgc aactctacga
     4261 ggacgagttc gtcatcgacc tgctcaccgt cgacggccgc tgcgttggcg cgctcaccct
     4321 caacctggcg gacggcgggc tcacgctgaa cgtcgcgatg gtgacggtgc tcgcgaccgg
     4381 cggcgccggg caggtgtacg ggcgtaccac caatccgtcg gtgagcaccg gcgacggcgt
     4441 ggccatggcc taccgcgccg gcgccgcgat ccgtgatctc gagttcgtgc agttccaccc
     4501 cacgggcctc gcggtccccg gccagcagac cgtgcagctc atcaccgagg cgttgcgcgg
     4561 cgagggggcc tacctgcgca actccgccgg ccagcgcttc atgaccgagg tcgacccgcg
     4621 cggtgagctc gcctcgcgcg acatcgtcgt gcgcgggatg gtcgccgaga tgcgtcgcga
     4681 gggcacccac cacgtctacc tggatgccac ccacctggac gccgcctggc tgcaggagcg
     4741 gttcccacgc gtgaccgccg gcctgcgcga ccacggcctc gacctggcga cggagctcat
     4801 cccggtggcg ccggtgtgtc actacttcat cggcggcgtc gtgacggacg tgtggggccg
     4861 caccacggtg ccgggcctgt acgccagcgg cgaggtcgcg agcacggggg tccacggcgc
     4921 caaccggctg gcgagcaact cgctgctcga gggactcgtg ttctccgacc gcgtcgtgcg
     4981 cgatctggat cgctacatcg ggcggctggg cgaggacgtg cgccgcttga gcttcgacat
     5041 ccccgaggcc acgccggacg cgggcggcag cgatgcgccc gccgcccgcc gccgcctcac
     5101 ccgcgtcctg caggaaaagg taggcgtgct gcggcgggcg gacgacctgc aggcggcgct
     5161 cgacgagctc ggccgcctca cgtcggagct ccgtttcggc atcatgggtg aggccgagta
     5221 cgaggtgctc aacatgctca cgctcgggac gcagatcgcc aagtgcgcgc tgctgcgcga
     5281 ggagtcgcgc ggcgtgcatc tgcgtgacga tttcccgggg ctggacgacg agcgctggcg
     5341 cacgcacatc acgctgcggc tgccggagca cgagcgcacg gaaggtgtgg gcagggtggg
     5401 gccgtcatga cgagcaggca ggccggcgag cgggccgccg gaaccggacc gctccgcgcc
     5461 cgcggctacc gtcaggccgc gcgcatggcc gcgcgccgcg ccctgcgcga ggacctcgcc
     5521 ggctacggcg acatcgccgg atcggccttc gacggccacg gcgcggcgcg cgtcgtgggg
     5581 cgtgagggcg gcgtcctctc cggcgtcgcc gcgctcgagg agacggccgc gctggtggat
     5641 ccggcgctcg ccgtgagagt cttcgtcgag gacggcgagc gcttcgcggc cggcgacgtc
     5701 gtcgcggagc tgcgcggccc gctctcggcg atcctggccg ccgagcgcac ggcgctcaac
     5761 ttcctctgcc ggctgagcgg tgtcgccacc ctgaccgcga cctacgtcgc cgagacggcc
     5821 ggcaccgggg cgcgcatcgc cgcgacgcgc aagaccacgc ctggccttcg cgcgctcgag
     5881 aagcaggccg tggtccacgg cgggggcacg cctcaccggt tcggcctctt cgacggcgcg
     5941 atgatcaagg acaaccacgt cgccgcggcc ggcggcgtcg ccgccgcagt ggagcgcgtg
     6001 cgcgccggcg cggcgcacct gcacgcgctc gaggtcgagg tggacacgct cgaacagctg
     6061 gatgaggcga tcgcggccgg cgccgccatc gtgctgctcg acaacatgga cacggcgacc
     6121 gtaggcgcgg ccgtcgcacg cgtggccggt cgcgccgtcg tcgaggtctc cggcggcgtg
     6181 cggctcgagc gcgtgcgcga gctcgccgaa gccggcgcgg acatcatctc cgtcggcgcg
     6241 ctcaccacgc gcgcgccgtg gatcgacctg gccctcgacc tggagacctg accgtgctgc
     6301 tggtcgtcga cgtcggcaac acgcagaccc acctgggggt catcgacggc ggcgcggtgg
     6361 tcgacgagtg gcgcatcgcc acggtccgcc accgcaccag cgacgagatc gccggcctgc
     6421 tgcagggctt cttctccctg cagagccgcc ggctcagaga ggtcgtcggc gaggtcggca
     6481 tcgcctccgt ggtcccgcgc ctcacgcagc agtgggagga gatgtgccgc aagtacctcg
     6541 gcatcccggc gttcgtcgtc ggccccgggg cgcgcaccgg catgcgcatc aggacgcgca
     6601 acccggccga ggtcggcgcc gaccgcatcg tcaacgccgt cgccgccttc gagatgtacg
     6661 gagggccgtg catcgtcgcc gactacggca cggccaccac gttcgacgtc atctccgccg
     6721 agggcgacta cctcggcggc gccatcgccc ccggcatcga ggtctcgctc gaggcgctca
     6781 cctcgcgcgc ggccaagctc atcaaggtgg agctcatcga gcccgagcat gccatcggca
     6841 agtcgaccac cgaggcgctg cagtccggcg ccgtctacgg gttcgccggc gaggtggagg
     6901 gcgtggtgca cgccatctgg gcggagatgg gagagaagtg cccggtgatc gccaccgggg
     6961 gcctggccga gatgatcgcc aggcacaccg acgtcatcga gaaggtagac cccttcctca
     7021 cgctgcgcgg catcgagatc gtgatgcggc gccagcagcg ggattgaggc gacgggcttg
     7081 acgggcgtgg cgacgcccgg gccgccgccc tccggcacgg gtccggcccg gtccgggccg
     7141 gccggcggtc cctgcgccac cgatccgctg ctccggccgc tcgccatcgg cggcctccgg
     7201 ctcgagcgcc gcctcttcat ggctcccatg gccggcctga ccacgtcggc cttccggcga
     7261 agcgtccgcc gctggggtgc cggcctcgtg ttcacggaga tgatctcggc ctacggggtg
     7321 cactaccaca accggcgcac gctggactac ttcgcgcacc gggaggacga gcacccgctg
     7381 gggttccagc tgttcggggc cgacgcggac gtgctcgccg acgcggcggg gcgggccgtc
     7441 gcggccgggg ccgacgtcgt cgacctcaac atggcgtgcc cggtccgcaa ggtcgtcaag
     7501 accggcgccg gcgcggcgct gctcgcggag ccggaccgcg ccgccgccat cgtcgccgcg
     7561 gtcgtggcgg cggtggagga cgccgcgccg gggcgcggcg tcccggtcac cgtcaagatc
     7621 cggtcgggcc tccgcgacgg tgacgggctt ggcagccgcg ccgcgccgcg cctcgccgcc
     7681 gccggcgccg ccgccgtctg catccacccg cggacggcgg cgcagctcta ccgcggccgc
     7741 gccgatcacg cggtgacgtt cgcgctcgcc gcggcactgc cggtgccggt gatcgcctcc
     7801 ggcgacgtcg acgggcggga ggcggcgctg gcgctgctcg acggcggcgc cgcggcggtc
     7861 atgctggccc gcggcgctgt cggcgcgccc tggctcttcg cgcaggtgct cggggaggcc
     7921 gagccctcgg ccgccggcaa gctcgaggag gtacgccgct tcgcggccga ggtcctcgag
     7981 gagtgcggcg gccgcggcgt cggccacctg cgtcagttct ggcccggatt ccgccgccac
     8041 ggcgccctgg acaaggccct ttcgctccag ctcatgcagg cgcgggacga ggccgggctg
     8101 cgtacgttgc tcggcctctg agatctcctc cggacgccgc cgctgcgcct ccgctcaggc
     8161 tcgcggcgga ggcgcccgcg ccgcgtttgc gcaaagactg ggcatgccct atacttgctc
     8221 gccacttcgg cacgcggctc cgaacggggc cgcgttgccg cgttgaagag gaggaatcag
     8281 tggtcgaccg cgagatcatc ctcacccccg agggcttcca gcgcctcaag gaggagatcg
     8341 agtacctctc gtccgtcaag cgcgacgagg tcgccgagcg tatccgggcc tcccgcgact
     8401 tcggcgacat ctcggagaac tcggagtacg acgacgcgaa gaacgagcag gcgatgctcg
     8461 aggcccgcat ctacgcgctc gaggagcgcc tgcgcagcgc catcgtcatc gactcggact
     8521 ccatcacgac cgacaaggtc ggcgtgggca ccaaggtcac ccttcaggac atgcagcgcg
     8581 gcgacgtcgt ccagtacgcc atcgtcgggt cggccgaggc cgatccgggc gcgcacaagc
     8641 tgtccaacga gtccccggtg gggaatgcga tcatgggcca caagcctggc gacaaggtga
     8701 cggtgagcgt cccccagggc tccaagaagt tcaaggtcct ggccatcgag aaggcctgac
     8761 ggaccgcgcc gtggacgagc agcacgccga ccacggcgac gccctcgccg agcggcgtga
     8821 caagctcacg gcctggcgcg gcgccggcgc cgcctacccc acgcgctacc agccgcgcga
     8881 cgagatcgcg cggctgcagg ccgcctacga gtcgctcgag tccggacagg actccccgga
     8941 cgtgcgccgg gtcgccggcc gcgtcgtcgc gcgccgcgtg cacggcaagc tgacgttcgt
     9001 cgtcgtcaag gacatgagcg gcgagttgca gctgttctgc cagctcgacg ccctcggcga
     9061 ggagcgctac cacgcgctgg agcacgtcga cctgggtgac ttcgtcggcg ccgagggcac
     9121 catgatgcgc acccggcgcg gcgagctcag cctgcgcatc acctcctggc agctcctcag
     9181 caagtcgctg cgcggcatgc cggagaagta ccacggcctc accagcgtcg agacgcggta
     9241 ccgcaagcgg tacctggacc tcgccgccaa cgacgactcg cgcgaggtct tcatcaagcg
     9301 cgcgcgcatc atcgccgcca tccgcaggtt cctggacgac cgcggcttcg tcgaggtcga
     9361 gacgccggtg ctgcagccgc tctacggcgg cgccctcgcg cgcccgttcg tcacgcacca
     9421 caacgagctg gagcgcgacc tctacctgcg catcgccacc gagctgtacc tcaagcgctg
     9481 catcatcggg ggcatcgacc gcgtgtacga gatcggcaag gacttccgca acgagggcgt
     9541 gagcttcaag cacaatcccg agttcacgat gctcgagacc tacgaggcct acgtcgacta
     9601 caaccacgtc atgacgatgg tcgaggagat gacggcgcac gcggccgagg ccgtcggatc
     9661 gctcaaggtc acgttccgcg agcacgacat cgacctcacg ccgccgtggc cgcgcctcac
     9721 catgcgcaag gccatcgaga acgagaccgg catcgacatc ggcgccgacg acgcgcgcac
     9781 cgtcgccgac ctgcaggcgc gcatcaagga gaaggggctc gaggatgtcg tgccgctcgc
     9841 ccccgcctgg ggcgcccagg tcgacgagct gttcagcgag tgcgtcgagc ccaagctcat
     9901 ccagcccgtg ttcattaccg agcacccgct cgagacca
//
LOCUS       DASUMX010000213        25931 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1370377, whole genome shotgun sequence.
ACCESSION   DASUMX010000213 DASUMX010000000
VERSION     DASUMX010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 25931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25931
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1370377"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="VFZ86_10205"
     CDS             <1..578
                     /locus_tag="VFZ86_10205"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEX5642690.1"
                     /translation="EPYRRERLVTFFDPWRDPQGSGFQATQSLISIASGRVFGVGLGN
                     SVQKFGFLPEQGTDMITGIIGEELGLVGLLVLLALYAALAWACFRIALNCKDLFGKLL
                     ATGITAMIVGQACINVGAALGLLPLTGVPLPLVSLGGTSMLVVLAGLGILLNIATNRR
                     SHIAVSAQRRDGAAGGGRDRRSPAAGARRRG"
     gene            490..1605
                     /locus_tag="VFZ86_10210"
     CDS             490..1605
                     /locus_tag="VFZ86_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007574552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5642691.1"
                     /translation="MPSALSDATVLLAAGGTGGHLQPALAVAAEAQARGARVVLVTTP
                     SQVERVRDQYRTYALELKGFERRLDVPAYARTFKLLGAAVPRVRRILRETRPDVAVGG
                     GGFASGPVVAVAAASGVPGLALEADAHLGVANRLLRPFVKRFCLSFPIEGLEPPRYVV
                     TGRPLGPGQLDATPEQGREAFALHAGLPVVLAFGGSQGAQSLNRACVDAFGGKELEFQ
                     LVHVCGPRNAEQVRRELEERGERFERYRLVAYTDRLAAAMAAADLVVARSGGSVAELA
                     ALGKPAILVPYPHATADHQRKNAREMVAGGAALLVDDAELDGALLARLVAELLGDRAR
                     LAAMAAASAALGRPDAAQRVADQIEDVLTTRRRRGAR"
     gene            1602..3014
                     /gene="murC"
                     /locus_tag="VFZ86_10215"
     CDS             1602..3014
                     /gene="murC"
                     /locus_tag="VFZ86_10215"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003540036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEX5642692.1"
                     /translation="MSHEAAKDFPQPVHFIGIGGAGMSGIAAVLHELGVDVRGSDLKT
                     SRYTRHLTEAGVPVTVGHDAANLGDAAVVVTSTAISAANPELRAALAAGLPVLRRAEM
                     LARVMELRRGIAVAGTHGKTTTSSMLSHVLRQAGLDPTFLVGGELNDIGSNAGVGQGE
                     WLVAEADESDGSLLFLRPEVAIVSNVELDHHANYGCLDDVHDVFRRFVALLPEDGLLV
                     LVAGSGGEALAAHTPAPVVVVGLEEGDLRAEVERVDDRGSVFTVRDAAGEALARVELR
                     VPGEHNVLNALTALAVLRHAGVSPEEAAPHLLTFSGAARRFQLIGEHDGVMVVDDYAH
                     HPTEIAATLTAAMKGRHERVIAVFQPHLFSRTRYLQREFGQALTLADEAIVTEIFPAR
                     EEPEPGVSGKLIVDAYLAERPGGPVSFLPRLGDVVARLASQVRPGDLVLTLGAGDVFH
                     AGEQLLVTLAGQGEAPAGAE"
     gene            3105..3980
                     /locus_tag="VFZ86_10220"
     CDS             3105..3980
                     /locus_tag="VFZ86_10220"
                     /inference="COORDINATES: protein motif:HMM:NF020067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HEX5642693.1"
                     /translation="MLLTLAILAVPTAVYAWGRTSSSFGIATIRVAGTHLVPEKRVLR
                     LLREEYAGRNLFGVTGGDVRKTLAPLCFVRGARIDRDFPETLAVTITEYEPAAYALAG
                     DRWYVVDRDGYVICSAAVAAEQLAKKPAKKPSPSPTASSAGAVEADAAAAALDPGEAA
                     GDGGSEQPGSVLERLIAGPGKAALALPRMAVTGKVREGRVVSDEAAAEMLSVIAALPR
                     SLQRSLTAVQHADGQLTLRFAGGPVTTWGDAERTLAKSVALRTVLREYADAGKRCTQL
                     DVSIPDRTLAKPVLQ"
     gene            4250..5338
                     /gene="ftsZ"
                     /locus_tag="VFZ86_10225"
     CDS             4250..5338
                     /gene="ftsZ"
                     /locus_tag="VFZ86_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEX5642694.1"
                     /translation="MIDASANYLAVIKVVGVGGGGTNAVNRMVDAGLRGVEFIAVNTD
                     AQALQMCDADVKIHIGSKITRGLGAGANPTIGYQAAMESKDELRDAIKGADMVFVTAG
                     KGGGTGTGAAPVIAELAKELSALTVGVVTRPFAFEGKRRARQASDGIQSLRDKVDTLI
                     VIPNDRLLQVVERRTSVIEAFKVADDVLRQGVQGITDLITVPGLINLDFADVRTIMKD
                     AGSALMGIGVAGGENRAVEAARAAVSSPLLEASVEGARGILLNITGGNDLGLFEINEA
                     AEIISSAADADCNIIFGAVIDPSLGDEVRVTVIATGFDREVPVEQPARQEVAEREPKG
                     TPLFAAESKPSFDVDDDVLDIPSFLRDH"
     gene            5450..7090
                     /gene="secD"
                     /locus_tag="VFZ86_10230"
     CDS             5450..7090
                     /gene="secD"
                     /locus_tag="VFZ86_10230"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEX5642695.1"
                     /translation="MRRHRNLIILVVMAALVAVSLYYIIPPSVKTRLGLDLQGGLEVV
                     YTARTADGKAPSQAQLDQTVGILDRRVNGLGVTESQIQTQGADQIAIALPGITDAEQA
                     LSVIGKTAQLEFFKDDPESRPVGPVESKEAALTQLRDQGVSKAEVEQLRQEGTSDDYS
                     LVVSPADAANEIPEQWSVYARPPAMTGEAVKSARAGFDNTTGKPNVLIDFTSDGSGQF
                     QEITRELYRTGLLKQVPQTFAIVLDNVMESDPMIDYTDPLLRDGISGGAEISGGNMTV
                     QESKDLAFVLNTGALPVKLETAYQQQVSATLGKDSLDQALVAGLAGLVLVLLYMLLFY
                     RFLGLVADLALIVYGILLWGLFNVIPVTMTLPGIAGLILTIGVAADANVVIFERIKEE
                     VSHGKTVRSAVNSGYALGFKTILDANILTMLTAVVLFIFATAQPKGFAVTLILGVLVS
                     MFTAVLFTRAILGVLAGFAFFNRPSLMGVSPSQIQAGRAAQASASRAAASRGRRAGAT
                     TTTEPATEGAVAGGEAAPAGRPPAPRRKSTSTKRKKRR"
     gene            7095..8042
                     /gene="secF"
                     /locus_tag="VFZ86_10235"
     CDS             7095..8042
                     /gene="secF"
                     /locus_tag="VFZ86_10235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEX5642696.1"
                     /translation="MSFFRRLYQFDYMGHKKWWFAFSGVLIVISLASLIFNGLEFGLE
                     FQEGTRVTVAFQTPPPLGDLRDAVSEAGYPNAQIQQTANVSGGAVEGFQIQTPMLSGE
                     QQVELRAALDEKFTLESSAGKPVWGIETVSASFGRQVVSSSLKAIAIALLLIVAYVTI
                     RFQWKYAVGGIAAVIHDTLIVVGIYSLTGREVTTATIAAVLTIMGYSLYDNVIVFDRI
                     RENEPKLGRMAYADMVNESLWETLTRSLNTSFMSLLPVTCLLLFGGETLKDFAFALFV
                     GIILGAYSTFFVASPVLTVLKEREPRYKKLKAQATADRA"
     gene            8261..8704
                     /locus_tag="VFZ86_10240"
     CDS             8261..8704
                     /locus_tag="VFZ86_10240"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX5642697.1"
                     /translation="MASRAIRSRETTAEQARLRLCAEGGDGRPPVVQLEGEFDLDTVA
                     EIDRFLRRALGPLYDQDHLVMDLSRTTFVDSSFVAFLVRLSAAQRAKRRELLLVRPAG
                     QVRKVFGIVGLANVVPVFETTEAAVDSLLNGRLPVIPPAFEAAGA"
     gene            complement(8778..9191)
                     /locus_tag="VFZ86_10245"
     CDS             complement(8778..9191)
                     /locus_tag="VFZ86_10245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642698.1"
                     /translation="MTRRPPGANTMEFRSTRALRGSRLQDVADVMLKTKTYGVGRICM
                     VDGCGTRLSAYNPSGVCALHGGAWQDEYHRTTRKATHREEITRHCLFGQCGREFTTTN
                     PAKKYCSDACRMRAFQARVMESRRMDDAVPIRRAS"
     gene            complement(9231..9590)
                     /locus_tag="VFZ86_10250"
     CDS             complement(9231..9590)
                     /locus_tag="VFZ86_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011438656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX5642699.1"
                     /translation="MEDKPQFRVDYEATTAEVGIVILEGEIDIYSAPEFKEVLVNGIE
                     GGAKRVIVDLSAVTFIDSTALGVLVSGAKRVRPRNGNLDIVCTDENIIRIFEITGLDR
                     IFGIFPSRGEALRAASA"
     gene            complement(9740..10543)
                     /locus_tag="VFZ86_10255"
     CDS             complement(9740..10543)
                     /locus_tag="VFZ86_10255"
                     /EC_number="2.3.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF019403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015019 - heparan-alpha-glucosaminide
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0007041 - lysosomal transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase"
                     /protein_id="HEX5642700.1"
                     /translation="MAGSQKVTGSSPVTSTIHSPSHSSPQGPTATRFWEIDALRGLAV
                     LAMIAFHFAWDWAYVNDGRLGDASRYASGMIAGTFITLLGLSIALDRARVRAAGGSQW
                     RRTGHRFLLIGGSAAVVTLATWLFLPDEFVFFGILHLLAACTVLVALSAPLGAVANAF
                     LGLALLVIGWSTLLDAPGPGALWSVAGWWAPRATVDWYPLAPWAGFAFLGLALGLTLY
                     AGGRRRFSLRDLSAPTAPLRFLGRHALPIYLVHQLLLLPLAWLLARLLR"
     gene            complement(10495..10570)
                     /locus_tag="VFZ86_10260"
     tRNA            complement(10495..10570)
                     /locus_tag="VFZ86_10260"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10535..10537),aa:Ala,seq:ggc)
     gene            10720..12201
                     /locus_tag="VFZ86_10265"
     CDS             10720..12201
                     /locus_tag="VFZ86_10265"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX5642701.1"
                     /translation="MIDGSGMESGHDPGPGRGLTVLALRIALLLVVVAGVLQLQAAAG
                     YVVATGSITFQVRPAWPGGHLVMPLGPAGELSLRTHRTPMDVIMDYRLPAQTAALLDD
                     PSAGLPRLEGGARAAFSRYLIGRLPWLLLVGAAAGLLVAGFRTRRRLVLAAAAGAAAA
                     MVLGGAFALASYATLDRSPEVRYSGLASRVPSVLPLLRALSSGGDQGDRLSRLQDFLD
                     GLEAVATQLTTEPRRPARAEVVRLLLASDIHDNVFGARAAARLAAGGGEPVDAVLLAG
                     DITDRGTAEEARLFVRVFGRGDPPVLLVGGNHEDEPALRVFRDAGYRVLEGTLEDVAG
                     VPILGASDPVASQPRVSSDEELLGVYGARLAGLWAAADPRPQVLLVHDARQAQDTITA
                     AAEEEAALVVAYGNDHVAGVHGEDGVVLVDAGTAGASGYEAIGAASPTSAPAREPPAG
                     SRSVYTFQLIDFSRSAEPRLTGVTTVSYTGTGRTVVTYTPFEP"
     gene            12340..14817
                     /gene="leuS"
                     /locus_tag="VFZ86_10270"
     CDS             12340..14817
                     /gene="leuS"
                     /locus_tag="VFZ86_10270"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEX5642702.1"
                     /translation="MDRRYDPHAIEEKWQRLWKERDAYRTPDESDKPKFYALDFFPYP
                     SGDGLSVGHCRNYVPTDAVARFMRMRGYNVLHPMGWDAFGLPAENYAIKMGVHPRVTT
                     ERNIANYHRQMDLIGLSFDWSREVTSCFPDYYRWTQWFFLLLYQRGLAYQATGQQWWC
                     PSCKTILANEQVEQGRCWRCGSLVTKKDLEQWYFRITDYAQRLLDDLGTIDWPEQIKL
                     MQTNWIGRSEGAEVDFGLQGRDEKLTVFTTRPDTLFGATYMVLAPEHPLVDALTDDEH
                     RAAVVEYQDQARRQSEIERMSTEKEKTGVFTGAYAVNPVNDAPVPIWISDYVLMGYGT
                     GAIMAVPAHDERDFAFATRFDIPIVEVIAPESGPQGALTEAYTGDGVMVNSAQFDGLQ
                     APGEAFEAVVAWLGERGAARKKVNFKLRDWLISRQRYWGAPIPIVHCEKCGTVPVPDD
                     QLPVLLPDLEDFHPTDDGRSPLARAEEWVHTTCPGCGGPAHRETDTMDTFACSSWYHF
                     RFASPHDEAAAFDREKTRYWLPVDLYVGGAEHAVMHLLYARFFCKVAQDAGYLDFGEP
                     YARLRNQGMIQAEDGQKMSKSKGNVVTPDSVVEKYGADALRVYELFIAPFEQSVAWSD
                     RGVQGTYRFLGRFWNLVGEVVEAQGSGVAAGEAEAEKGRQVLRALNKTIRKVTRDMDA
                     FRFNTAVSALMELQNQVLDVWSSARGSLTLAQWREVVRDMTLLVCPMAPHVAAEVWEL
                     LGHGDDVLDAPWPAWDEELAADAVVTVVVQVNGKLRDRLEVPVDAEKDDVLAMARAAE
                     NAARFLDGKQVVKEIYVPGKLVNFVVR"
     gene            complement(14814..15239)
                     /locus_tag="VFZ86_10275"
     CDS             complement(14814..15239)
                     /locus_tag="VFZ86_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642703.1"
                     /translation="MTSKAMFTDDEWHTLQWAVTDTMAYVSMADAGFWDTFKEASAAA
                     KFMAQAKASSGSPLVHDLAGDIKFGRDKAVSHDMADMGGEVTARVRQAVEVVAAKSPA
                     DLTAFKGFLLGVAQATADAVKGVGDHEAAAIAKLQDVLF"
     gene            15431..16087
                     /locus_tag="VFZ86_10280"
     CDS             15431..16087
                     /locus_tag="VFZ86_10280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEX5642704.1"
                     /translation="MQLSRRQIIAYVAVAAVVVAVGVRYVVLPRQAGPPQAQAVVLAP
                     VAASPASQDAGASAGATAASPAPDMMVYVCGAVRAPGVVRLPPGARVTDALQVAGGPT
                     AKAELAAVNLAAPVSDGQQIVVPERGAAVVAAQASGGSSTGGAPALAPAAAAGGLINI
                     NTASLEELDALDGVGPSTAQKIIDYRTANGGFTTIEEIKEVPGIGDAKFAAMQDAITV
                     "
     gene            16097..18469
                     /locus_tag="VFZ86_10285"
     CDS             16097..18469
                     /locus_tag="VFZ86_10285"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEX5642705.1"
                     /translation="MRLPAPPHVLLGAYCLGLCLVLAWRPPPELLAAAAALAAGALIV
                     GVRAPALSGRGPVAGLAGLAALFLCAGAVLGGARLDAVARSSLAAYTGDRVSLAAVLT
                     DLPAIDADQVTLALRVTDVGGRRLREPAHLRLRLEDDKAFAHDPTGPLTEGALLQVAS
                     VRVAPLPEPRPGAFDYGRYLRRRGEHVLLEGDFADLVVTGRREGLQGMVDRLRLASRA
                     HLAAGVHSPVADVLQGMVLGDDEGVDAALVEDFRASGLLHIMAVSGENVVLLCTMWSF
                     AFALLGMHRLLRTALLLPVVATYVLLTGASPSIVRAGVAGICGLLAILASRPSDGWLL
                     WLAPAAWLLTANPNNLFDVSFQLSFGAVVGLLVLAGPLTRAVSFLPGPLPEQVGVTTA
                     ASISTAPVSMLTFGSASLVSVPANVAGGFVLGPIMFLGMLSLLLGFVSEWLSAPLNVV
                     AGLFIGFLLAVSHLFAGLPGAVYEYQGVSLRLLLVLGLLAEIALVGALAARAGEGVLA
                     YVRDRRRWAPLAGATAALLAAALLLAPAPVRPPDVPTLTFLDVGEGAATLLQVPGGPT
                     VLVDAGPEALARTLREHAVGEVDLLVLSHGHDDHVGGLADVIGAVTIRAALLPQPEEP
                     SAALDDLEDRLRAAGTDVRRCTTDLRLGGAGWSLDVLPSAPVGGVDQNQRENDDALVA
                     VVGLAGQRVLLPGDAEGQVLENLDLPPCLVVGVPHHGSDGGFDAGFLRQLAPRLAVVP
                     VGPNRHGHPDQDALAVLTAAGVPYARTDRSGDIALSAGSEGLSVTAEREG"
     gene            complement(18476..19240)
                     /locus_tag="VFZ86_10290"
     CDS             complement(18476..19240)
                     /locus_tag="VFZ86_10290"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5642706.1"
                     /translation="MSGLVPFLRKEFSETVRTWRLWVVPGFLLFSALSSPLVTYLMPT
                     LLDQLGTAQQGLSITVADPTALQAYLEYLGNLGQLTLFALVIAYGGIVSGEVRGGTAA
                     LTLAKPLSRAAFVTGKWLSQALVVAAGAAFGTLICIALTQLLFEDGPAGRTVLAVGLW
                     IAYALMLLAVMVLLSVELKAPAAASGAGIGVYAALVVLAQFEATSGATPAGLPAAGLA
                     IVRGEPAQWALPLVTTVAVAAACLVAAVLRFERREI"
     gene            complement(19237..20655)
                     /locus_tag="VFZ86_10295"
     CDS             complement(19237..20655)
                     /locus_tag="VFZ86_10295"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5642707.1"
                     /translation="MLTAGAAVHGSGVTLVETTLPIATHVAATGTSPALVALLVVLGA
                     VEVGLLAWALVDLYRRPSAQINGGSKIPWLILCLLLQIIGPIVYLAAGRERVKPAHDP
                     LAGGGPAGSAAGRAVAGGATVAGVAAAGAVPPPLAVQRADAATVADALFAARGAEPDG
                     AAASGPAVELRDVRKTFGDAVALDGLTLSVPPGSIYGFLGPNGAGKTTTLRILAGLAH
                     ADAGAVRILGRDVAGGGADARAVIGFLPDVPAFYKWMTAPEYLRFAGSLFGLSGRELD
                     DRVTAMLELAGLAGVRTRVGGFSRGMKQRLGVAQALIHGPRLLLLDEPTSALDPIGRR
                     DVLDMIASMRGRTTVFFSTHILSDVERVCDTVAVIARGRVVAQSGIDELRSRRGGLNR
                     LSIEVDDPRRLWEGLAGAPWLRTVEAGESGELLVTCDDVGAAQRELPALVGRLGLALR
                     RFGVDELDLEDVFVDLVGEDRR"
     gene            20684..21691
                     /gene="holA"
                     /locus_tag="VFZ86_10300"
     CDS             20684..21691
                     /gene="holA"
                     /locus_tag="VFZ86_10300"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEX5642708.1"
                     /translation="MAEPTPLLPAYLIVGTDRPKVRRAVVRLRQRVIDEAGSDLNVVL
                     LDAEHDPVEAFLDAAVSPGLTFGTRLLLVLNGHKWKAKARQAVVAYLQDPMPDTCVTV
                     EGETFAASDALYKAVSKLGGVLRFDLPKKYEMAKWVRERAKAHRLPLSQIVAKHLLDR
                     CGTDPRHADRLEREIEKLALYCRGQEATEADVDAICTPDDDAVIFDLMDAVGHRDRTH
                     GFALLEALYASGNPRNDANGVLYSLKRHIEKLDAASQLPHADQTTAAKQLGVHPFTAK
                     KLLEQREHFDRRRLGRAYRALAEAEAGLRGRAPATLESIGGVNDGERLVVELALARLL
                     T"
     gene            complement(21806..22072)
                     /gene="rpsT"
                     /locus_tag="VFZ86_10305"
     CDS             complement(21806..22072)
                     /gene="rpsT"
                     /locus_tag="VFZ86_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004004703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEX5642709.1"
                     /translation="MANIKQQKKRIRLAAKQRLRNRHAKSTLKTLFKQLEDEVAEGQT
                     EEAAKTATFLTSRIDKAAAKGILHKNNAAHKKSRVAHTMARLSS"
     gene            complement(22211..23347)
                     /locus_tag="VFZ86_10310"
     CDS             complement(22211..23347)
                     /locus_tag="VFZ86_10310"
                     /inference="COORDINATES: protein motif:HMM:NF012907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HEX5642710.1"
                     /translation="MTRASREVRAARLRRQRLLVLLLAAGALAVVVVLLFTVGPFGSH
                     LDRPGDPPLGYLIEPLGGRQHRVTAWTLGDAASLTSATAANAVDEIDFDWYHTQADGS
                     VTAENEDPALVGAARDKGLNIFATVTNSTSSGSAFSRDVAAAVLASPDRRRRFIDDLL
                     TLVEDMGYDGVDLDWEELRPADRDSFSVLVEELAEALHAEQRFLSIAVMPKTSEPGEW
                     DNQKYADWKRIGKAVDEFKIMTYSYSGPWGDPGPQAPAEWVDRVLTFAERKVPPRKIS
                     MGIPFYGFDWYGDSCDTVSAHRGASLAQTHDATVGRDPSSDEATMTFTDGGVTHTAYF
                     QDEAAVAAKLAALRAGHPGIAGISAWVMGQEASGFWPLITRELR"
     gene            23576..24754
                     /locus_tag="VFZ86_10315"
     CDS             23576..24754
                     /locus_tag="VFZ86_10315"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEX5642711.1"
                     /translation="MTGLRQTLATLGLLAGATVALATGGCASEHVAQAALEPTSEAAL
                     ASASPSSSPSPSPSAPSELAGWGNRRLAAQLVVCSVPATNPLSGCRYARMGVGGIVIL
                     GGGARSSIGGDLAAVLRAARGGVKPLIASDEEGGQVQRLRYVIYPLPSPRVQGGWSAA
                     KTRATAKDYGARLRKLHVSVVFGPVADLDIEGRYMSSLDRCFSPRPDVVGSHVLAWVS
                     GLRASRVLATVKHWPGHGWATDTHSGAARVPSLSVLRRYDMVPFEKAFDAGVPLVMVG
                     HLRSKGLTGDETPASLSRTAMTYLRGRISDKTVIITDSLSMAATTKAVGLRLPAAAVR
                     ALKAGADLALFATGDPGPVIDAVTAAIKSGRIPRAEAEAKVLRVLELKRSAGLAPAAQ
                     "
     gene            24881..>25931
                     /locus_tag="VFZ86_10320"
     CDS             24881..>25931
                     /locus_tag="VFZ86_10320"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HEX5642712.1"
                     /translation="MSDHKRRLALAAVIVASATAVSRLTGLGREVITAAVYGVDASYN
                     TFVSVSVIPQLVQQLFADAAISAAFVPVFTALLAKGERDRAYRLAANLLGLIVVVVGA
                     AVVILALAAGPLTQWMYPELTGTPAGADLAADLLRILVPTILFLSLAGAISGVLYSFE
                     RFTMPAVVSIVWNLTIIAAIAIFNDTLGVDAIAWGMLVGTVLEVVMLAGAARYHGRWL
                     WPGFGLTDPLLRRVLLLMVPITITLGVLNFNALIGTWFAQFVSDRAAAEIGYAFRLYQ
                     LPQGIFAVTIGTVLFPSLSRFAAQKDETRFRDTVSMGVRQMVFVSLPFVAWFLVMPAA
                     FVQLVYERGQFGADAT"
BASE COUNT         3598 a         9294 c         9327 g         3712 t
ORIGIN      
        1 tggagccgta ccggcgggag cgtctggtca ccttcttcga cccgtggagg gacccgcagg
       61 gcagcgggtt ccaggcgacg cagtcgctga tctccatcgc ctcggggcgg gtgttcggcg
      121 tcgggctcgg caactccgtg cagaagttcg gctttctccc ggagcagggc accgacatga
      181 tcaccggcat catcggcgag gagctcgggc tcgtcggcct cctcgtcctg ctcgcgctct
      241 acgcggcgct ggcgtgggcc tgcttccgca tcgccctcaa ctgcaaggac ctgttcggca
      301 agctgctcgc caccggcatc acggcgatga tcgtcggcca ggcgtgcatc aacgtcgggg
      361 ccgcgttggg gctgctcccg ctcaccgggg tgccgctccc gctggtctcg ctcggcggca
      421 ccagcatgct ggtggtgctg gccggcctcg ggatcctgct caacatcgcc accaatcgaa
      481 ggagtcacat tgccgtcagc gctcagcgac gcgacggtgc tgctggcggc gggcgggacc
      541 ggcggtcacc tgcagccggc gctcgccgtc gcggctgagg cgcaggcccg cggcgcccgc
      601 gtcgtcctgg tgacgacgcc gtcgcaggtc gagcgcgtcc gcgaccagta caggacctac
      661 gcgctcgagc tcaaagggtt cgagcgccgc ctcgacgtgc ccgcctacgc gcggaccttc
      721 aagctcctgg gcgccgcggt gccgcgcgtg cgccgcatcc tccgcgagac gcgcccggac
      781 gtggccgtcg gcggcggcgg cttcgccagc gggcccgtcg tggccgtcgc cgccgcgagc
      841 ggcgtccccg ggctggccct cgaggccgac gcccacctcg gcgtcgccaa ccggctgctc
      901 aggccgttcg tcaagcggtt ctgcctgagc ttccccatcg aggggctcga gccgccccgc
      961 tacgtcgtca cggggcggcc gctcgggccg gggcagctcg acgcgacgcc cgagcagggg
     1021 agggaggcct tcgcgctgca cgcgggcctg ccggtcgtgc tcgcgttcgg cggcagccag
     1081 ggggcgcaga gcctcaaccg cgcctgcgtc gacgcgttcg gcggcaagga gctggagttc
     1141 cagctcgtcc acgtctgcgg gccgcgcaac gccgagcagg tgcggcgcga gctcgaggag
     1201 cgcggggagc ggtttgagcg ctatcgcctg gtcgcctaca ctgaccgctt ggccgccgcc
     1261 atggcggcgg ccgatctggt cgtggcgcgc tcgggaggat ccgtcgccga gctcgccgct
     1321 ctcggcaagc cggcgatcct ggtgccgtac ccgcacgcca ccgccgacca ccagcgcaag
     1381 aacgcgcgcg agatggtcgc cggcggcgcg gcgctcctgg tggacgacgc cgagctggac
     1441 ggagcgctgc tggcgcgcct cgtcgccgag ctgctcggcg accgggcgcg cctggccgcg
     1501 atggcggccg cgagcgcggc gctcggcagg ccggacgcag cgcagcgggt ggccgaccag
     1561 atcgaggacg tcctgaccac gagacgacga cggggggcgc gatgagtcac gaggcggcga
     1621 aggacttccc gcagccggtc cacttcatcg ggatcggcgg cgcgggcatg agcggcatcg
     1681 ccgccgtgct ccacgagctc ggcgtcgacg tgcgcggctc ggacctcaag acgtcgcgct
     1741 acacgcggca cctcacggag gccggggtcc ccgtcaccgt cgggcacgac gcggccaacc
     1801 tcggcgacgc cgcggtggtg gtcacctcca cggcgatctc cgcggccaat ccggagctgc
     1861 gcgccgcgct ggcggccggg ctgcccgtcc ttcgccgggc ggagatgctc gcccgtgtca
     1921 tggagctgcg ccggggcatc gccgtggccg gcacgcacgg caagacgacc acgtcctcga
     1981 tgctctcgca cgtcctgcgt caggccggcc tcgaccccac gttcctggtg ggcggcgagc
     2041 tcaacgacat cggctccaac gccggcgtgg gccagggaga gtggctggtc gccgaggcgg
     2101 acgagagcga cggcagcctg ctcttcctgc gtcccgaggt cgcgatcgtg agcaacgtgg
     2161 agctcgacca ccacgccaac tacggctgtc tggacgacgt gcacgacgtc ttccggcgct
     2221 tcgtcgctct cctgccggag gacggcctgc tggtcctggt ggcggggagc ggtggggagg
     2281 ctcttgccgc gcacacgccc gcgcccgtcg tggtggtcgg cctcgaggag ggggacctgc
     2341 gcgcagaggt ggagcgggtc gacgaccgcg gcagcgtctt caccgtgcga gacgcggccg
     2401 gggaggcgct cgcccgggtg gaactgcggg tgccgggcga gcacaacgtg ctcaacgcgc
     2461 tgacggcgct ggccgtgctg cgccacgccg gcgtctcgcc agaggaggcg gcgccgcacc
     2521 tgctcacgtt cagcggcgcc gcccgccgct tccagctcat cggcgagcac gacggcgtga
     2581 tggtggtcga cgactacgcc caccatccca cggagatcgc cgccacgctc accgccgcga
     2641 tgaagggccg acacgagcgc gtcatcgccg tgttccagcc gcatctgttc tcgcgcacgc
     2701 gctacctgca gcgcgagttc gggcaggcgc tcacgctcgc cgacgaggcg atcgtcaccg
     2761 agatcttccc ggcgcgcgag gagcccgagc cgggcgtgag cggcaagctc atcgtcgacg
     2821 cgtacctcgc cgagcggccc ggcggccccg tgtcgttcct gccgcggctg ggcgacgtgg
     2881 tcgcccggct ggcgtcacag gtgcgcccgg gcgacctcgt gctcacgctc ggcgccggcg
     2941 acgtgttcca cgccggcgag cagctgctcg tcacgctcgc ggggcaaggg gaagcccctg
     3001 ccggcgccga ataatctggg gtacttcgca acccccgacc gagggccggc gccatgacga
     3061 gggcacgccg ggcgagccgc ccgcgattcc gtctgctacc taccgtgctg ctcacgctgg
     3121 ccatccttgc ggtgcccacc gcggtgtacg cctggggccg cacctcgtcc tcgttcggca
     3181 tcgccacgat ccgcgtggcc ggcacgcacc tcgtccccga gaagcgcgtg ctccgtctgc
     3241 tgcgggagga gtacgccggc cgcaacctct tcggcgtcac cggcggcgat gtgcgcaaga
     3301 cgctcgcgcc gctctgcttc gtccgcggcg cacgcatcga ccgcgacttc ccggagaccc
     3361 tggcggtcac gatcacggag tacgagccgg cggcctacgc cctggccggc gaccgctggt
     3421 acgtggtcga ccgcgacggc tacgtcatct gctccgcggc cgtggcggcg gagcagctcg
     3481 cgaagaagcc ggccaagaag ccgtcgccct cgccgaccgc gagctccgcc ggcgccgtcg
     3541 aggccgatgc ggccgcggcg gcgctggacc ccggggaggc cgccggcgac ggcggatccg
     3601 agcagcccgg ctccgtcctc gagcggctga tcgccgggcc ggggaaggcc gcgctcgcgc
     3661 tgccgcgcat ggccgtcacc ggcaaggtgc gtgaggggcg cgtcgtctcc gacgaggcgg
     3721 ctgcggagat gctctccgtg atcgcggccc tgccacgctc actgcagcgc agtctcaccg
     3781 ccgtgcagca cgccgacggg cagctcaccc tgcgcttcgc cgggggcccg gtgaccacat
     3841 ggggcgacgc ggagcggacg ctcgccaagt cggtggccct gcgcaccgtg ctcagggagt
     3901 acgccgacgc gggcaagcgg tgtacccagc tggacgtgtc catcccggac aggacgctcg
     3961 ccaagccggt cctccagtga cggtggagcc tcttctcaac ctgtggtcga gccggggcgc
     4021 ctcgacggtc catccgcagg tctgtttgac acccctcagg ggcgtgggta aggtaaggcg
     4081 acggtcgggc cggcaggtct tccgggacgt ccacggatgt caggaatccc tgcaaacagc
     4141 gtgagtttgc ggcaccgatg aagatgaaac gagcaacact caggcggggc ttgagggttc
     4201 gccaggagtc acgacggcga gtctcgacca cgacaggagg gtcagcacga tgatcgacgc
     4261 gtcagcgaat tatctcgccg tcatcaaggt cgtgggcgtg ggcggcggcg gcaccaacgc
     4321 cgtcaaccgc atggtcgacg cagggctccg cggcgtcgag ttcatcgccg tcaacaccga
     4381 cgctcaggcg ctgcagatgt gcgacgccga cgtcaagatc cacatcggca gcaagatcac
     4441 gcgcggcctc ggcgccggcg ccaaccccac catcggctac caggccgcga tggagagcaa
     4501 ggacgaactg cgcgacgcca tcaagggcgc ggacatggtc ttcgtgaccg caggcaaggg
     4561 cggcggcacc ggcacagggg ccgccccggt catcgccgag ctggccaagg agctctcggc
     4621 gctcaccgtc ggtgtcgtca cgcgcccgtt cgccttcgag ggcaagcgcc gcgcgcgcca
     4681 ggcctcggac ggcatccaga gcctgcgcga caaggtggac acgctcatcg tcatccccaa
     4741 cgaccggctg ctgcaggtgg tcgagcggcg cacatcggtc atcgaggcct tcaaggtcgc
     4801 cgacgacgtg ctgcgccagg gcgtccaggg catcaccgac ctcatcaccg tcccgggcct
     4861 catcaacctc gacttcgccg acgtgcgcac gatcatgaaa gacgcgggct cggcgctgat
     4921 gggtatcggc gtggccggcg gcgagaaccg cgccgtcgag gcggcgcggg cggcggtcag
     4981 ctcgccgctg ctggaggcca gcgtcgaggg cgcccgcggc atcctcctca acatcacggg
     5041 cggcaacgac ctcgggctct tcgagatcaa cgaggcggcg gagatcatca gcagcgccgc
     5101 cgacgccgac tgcaacatca tcttcggcgc cgtcatcgac ccgtcgctgg gcgacgaggt
     5161 ccgcgtcacg gtcatcgcga ccgggttcga ccgcgaggta cccgtcgagc agccggcgcg
     5221 gcaggaggtc gcggagcgcg agcccaaggg cacgccgctc ttcgccgccg agtccaagcc
     5281 gtcgttcgac gtggacgacg acgtcctgga catcccgagc ttcctgcgcg accactgaac
     5341 ccggaggtgc gatccggcgt ctcctcgcgt gccaagttgc gacgctctcg tgcgtccggt
     5401 acgctctacc gcctgacggc ccagccaggg ccgcaggctt cgtgaacacg tgagaagaca
     5461 ccgtaacctc atcatcctcg tcgtcatggc cgcactcgtg gcggtgtcgc tgtactacat
     5521 catcccgccg agcgtgaaga cgcggctcgg gctcgacctt cagggcgggc tcgaggtggt
     5581 gtacacggcg aggaccgccg acggcaaggc gccttcacag gcgcagctcg accagaccgt
     5641 cgggatcctc gaccgccgcg tcaacggcct cggcgtcacc gagtcccaga tccagacgca
     5701 gggcgccgac cagatcgcca tcgcactgcc tggtatcacg gacgcagagc aggcgctctc
     5761 ggtgatcggc aagaccgccc agctcgagtt cttcaaggac gatcccgagt cgcgtcccgt
     5821 cggcccggtc gagagcaaag aggccgccct cacgcagctt cgcgaccagg gcgtctcgaa
     5881 ggccgaggtc gagcagctcc gccaggaggg cacctccgac gactattctc tcgtcgtgag
     5941 tccggcggac gcggccaacg agatccccga gcagtggtcc gtgtacgcgc ggccgccggc
     6001 gatgacggga gaagccgtca agagcgcccg cgccggcttc gacaacacga ccggcaagcc
     6061 caacgtgctc atcgacttca cgagcgacgg cagcgggcag ttccaggaga tcacacggga
     6121 gctgtaccgg accggcctcc tcaagcaggt cccgcagacg ttcgccatcg tgctcgacaa
     6181 cgtcatggag tcggacccga tgatcgacta caccgaccct ctgctgcgcg acggcatcag
     6241 cggcggcgcg gagatcagcg gcggcaacat gaccgtccag gagtccaagg acctggcctt
     6301 cgtgctcaac accggcgccc tgcccgtcaa gctcgagacc gcctaccagc agcaggtctc
     6361 cgcgaccctc ggcaaggact ccctcgacca ggcgctcgtc gccggcctgg ccggcctcgt
     6421 cctggtgctc ctctacatgc tcctgttcta ccggttcctg gggctcgtcg ccgacctcgc
     6481 gctgatcgtc tacggcatcc tgctctgggg gctcttcaac gtcatcccgg tgacgatgac
     6541 actgcccggc atcgccggtc tgatcctcac catcggggtg gcggccgacg ccaacgtcgt
     6601 catcttcgag cgcatcaagg aagaggtcag ccacggcaag accgtgcgct ccgcggtcaa
     6661 ctcgggctac gcgctcggct tcaagacgat cctcgacgcg aacatcctca ccatgctcac
     6721 ggcggtcgtg ctcttcatct tcgcgacggc gcagcccaag gggttcgccg tcaccctcat
     6781 cctgggcgtg ctcgtcagca tgttcacggc cgtgctcttc acgcgagcca tcctcggcgt
     6841 gctggccgga ttcgcgttct tcaaccggcc gtcgctcatg ggggtgtcgc cgtcgcagat
     6901 ccaggctggg cgtgccgcgc aggcgagcgc gagccgtgcg gcggcgtcgc gcggtcggcg
     6961 cgccggcgcc acgaccacca cggagccggc caccgagggc gctgtcgcgg gcggcgaagc
     7021 cgctcccgcg ggacggccgc cggcgccgcg ccgcaagtcc acctcgacca agcgcaagaa
     7081 gcggaggtag cggggtgtcc ttcttccgcc ggctgtacca gttcgactac atgggccaca
     7141 agaagtggtg gttcgccttt tcgggcgtcc tcatcgtcat cagcctcgcc agcctcatct
     7201 tcaacggtct ggaattcggg ctcgagttcc aggaggggac gcgcgtcacc gtcgccttcc
     7261 agacgccgcc gcctctcgga gacctccgcg atgcggtgag cgaggccggg tacccgaacg
     7321 cgcagatcca gcagaccgcc aatgtgtcgg gcggcgccgt cgaaggcttc cagatccaga
     7381 cgccgatgct ctcgggcgag cagcaggtcg aactgcgcgc cgcgctcgac gagaagttca
     7441 cgctcgagag ctcggccggc aaaccggtct ggggcatcga gacggtgagc gcctcgttcg
     7501 gccggcaggt cgtgagctcc tcgctcaagg cgatcgccat cgcgcttctg ctcatcgtcg
     7561 cctacgtcac gatacgcttc cagtggaagt acgcggtcgg gggcatcgcc gccgtcatcc
     7621 acgacacgct gatcgtcgtc ggcatctact cgctcacggg ccgcgaagtg accaccgcga
     7681 ccatcgccgc cgtcctcacg atcatgggct attcgctcta cgacaacgtc atcgtcttcg
     7741 accgcatccg cgagaacgag cccaagctcg gccgcatggc ctacgccgac atggtcaacg
     7801 agtcgctgtg ggagacgctg acgcgctcgc tcaacacgtc gttcatgagc ctgctgccgg
     7861 tcacgtgcct gctcctcttc ggcggggaga cgctcaagga cttcgccttt gcgctcttcg
     7921 tcggcatcat cctcggcgcg tactccacct tcttcgtcgc gagcccggtg ctcacggtgc
     7981 tcaaggagcg cgagccgaga tacaagaagc tcaaggcgca ggccacggcc gaccgggcct
     8041 gacgcacctc ggctccgacc ctccagtcgc ggcgcatgcc gcctcgacgt gaggtcgagt
     8101 gtggggtgcg cggaacgcca tcgttcaggc ttcgcgagag gctgacgcga gaacctctcg
     8161 atactgccac gtttacgctt tacttccgcg cggtgctgcc ctagcgtcgc tcacgcgccc
     8221 cgccggggcg cgctccatcc cgtcagcacg aaggggatcc gtggcgagtc gagcgatccg
     8281 atcgcgggag accaccgccg aacaggcgcg gctgcgcctg tgcgcagagg gcggggacgg
     8341 cagaccgccc gtggtgcagc tcgagggcga gttcgacctc gacacggtgg ccgagatcga
     8401 ccgcttcctg cgcagggcgc tgggtcccct gtacgaccag gaccatctgg tgatggacct
     8461 ctcgaggacc acgttcgtcg actccagctt cgtcgcgttc ctcgtgcggc tgtcggcggc
     8521 gcagcgggcc aagcggcgcg agctgctgct ggtgcgcccg gccgggcagg tgcgcaaggt
     8581 gttcggcatc gtcggcctgg ccaacgtcgt gcccgtgttc gagaccaccg aggccgcggt
     8641 ggacagcctg ctcaacgggc gcttgccggt gatccccccg gccttcgagg ccgcgggcgc
     8701 ttaggccgca agcgggtcag gccgccccgg cgccgccggc ggagccggcg ggcgcacgcg
     8761 gcgcggcgca gggagtctca gctcgcgcgc cgtatgggga cggcgtcgtc catacggcgc
     8821 gactccatga cgcgggcctg gaaggccctc atgcggcagg cgtcgctgca gtacttcttg
     8881 gcggggttgg tggtggtgaa ctcccggccg cactgcccga agaggcagtg ccgcgtgatc
     8941 tcctcacggt gggtggcctt gcgcgtagtg cggtgatact cgtcctgcca cgccccgccg
     9001 tgcagcgcac acacgcccga cgggttgtac gcggagagac gggtaccgca cccgtccacc
     9061 atgcagatcc gccccactcc gtatgtcttc gtcttcagca tgacgtcggc cacgtcctgg
     9121 agcctgctgc cccgaagcgc tctcgtgctg cggaactcca tcgtgttcgc ccccggcggc
     9181 cgccgtgtca ccctccggcg ccgcctcctg gtcgtgctgc ggcgtctcgc ctaggctgac
     9241 gccgccctga gcgcctcgcc ccggctgggg aagatcccga agatccggtc gaggccggtg
     9301 atctcgaaga tgcggatgat gttctcgtcc gtgcagacga tgtcgaggtt gccgttgcgg
     9361 ggccgcaccc gcttcgcgcc gctcacgagc accccaagcg cagtggagtc gatgaacgtg
     9421 accgcggaca gatcgacgat cacccgtttc gcgcctcctt cgatgccgtt gaccagcacc
     9481 tctttgaact ccggcgcgga gtagatgtcg atctcgccct cgaggatcac gatgccgact
     9541 tcggcggtgg tggcctcgta gtcgacacgg aactgcggct tgtcctccat acgcgcctcc
     9601 tccggccagc acggttcgtg gcagccttga cggcctccga gtgcatgtgc ccggatactt
     9661 tccgtgagga ggtgccgcta aacccgccgt cccaggcccg cagacggcgg tgacgagggg
     9721 cggcggtggc cgccggcggt cagcgcagca gtcgcgccag cagccaggcc agtggcagca
     9781 gaagcaactg gtggacgagg tagatcggca gcgcgtggcg gcccagaaag cgcagcgggg
     9841 ccgtgggcgc gctgaggtcg cgcagcgaga agcgtctgcg gccgcccgcg tagagcgtca
     9901 gcccgagagc gagaccgagg aaggcgaacc cggcccacgg cgccaggggg taccagtcga
     9961 ccgtggcgcg aggcgcccac cagccggcga ccgaccacag ggcgccgggg cccggcgcgt
    10021 cgagcaacgt gctccagccg atcaccagca gggcgaggcc gaggaacgcg ttggcgaccg
    10081 cgccgagcgg ggcggacagc gccaccagga cggtgcacgc ggccagcagg tggaggatgc
    10141 cgaagaagac gaactcgtcg ggcaggaaga gccaggtggc cagggtgacg acggccgcgg
    10201 agcctccgat gagcaggaag cgatgacccg tgcggcgcca ctgggagcct cccgcggcgc
    10261 gcacgcgggc gcggtcgagc gcgatggaga ggccgagaag ggtgatgaag gtccccgcga
    10321 tcatgcccga ggcgtaccgc gaggcgtcac caagccgccc gtcgttgacg taggcccagt
    10381 cccaggcgaa gtggaaggcg atcatcgcca gtaccgccag accgcgaagc gcgtcgatct
    10441 cccagaagcg cgtggccgtg gggccttgcg gcgacgaatg tgacggtgag tggatggtgg
    10501 aggtaaccgg gctcgaaccg gtgacctttt ggctgccagc caaacgctct cccaactgag
    10561 ctataccccc accgaagggg tgccgaccgt gccggcaggc tcgaaagtat agcatacccg
    10621 ctctcgccgg caacctgtcg cccgggcttg ggagcgcgga cgcggaagcg ggcagaaccg
    10681 gagtgaacga aggagcgatg acggcgtggc aggcagctgg tgatcgacgg cagcgggatg
    10741 gagtcggggc acgacccggg cccgggacga gggctcacgg tgctggccct ccgcatcgcg
    10801 ctgctgctcg tggtcgtggc cggcgtgctg cagttgcagg ctgccgccgg ctacgtcgtg
    10861 gccacggggt cgatcacctt ccaggtccgg ccggcgtggc cgggtggaca cctggtgatg
    10921 ccgctggggc cggcgggcga gctctcgctc cgcacccacc ggaccccgat ggacgtgatc
    10981 atggactacc gcctgccggc gcagaccgcg gcgctgctcg acgacccttc cgccgggctg
    11041 ccgcggctcg agggcggcgc ccgtgcggcg ttctcgcgct acctgatcgg caggctgccg
    11101 tggctgctgc tcgtgggcgc ggccgccggg ctgctcgtgg cgggcttccg gacgcgccgc
    11161 cgcctcgtcc tggcggccgc cgcaggcgcc gccgccgcca tggtgctcgg cggcgccttc
    11221 gccctggcga gctacgcgac gctggaccgc tcgcccgagg tgcggtactc cgggctcgcg
    11281 tcgcgcgtcc cgtccgtcct gcccctgctc cgcgcgctga gcagcggcgg cgaccagggc
    11341 gaccgtctca gccgcctgca ggacttcctg gacggtctgg aggcggtggc gacgcagctg
    11401 acgacggagc cgcgccggcc ggcgcgcgcc gaggtcgtgc ggctgctgct cgcgagcgac
    11461 atccacgaca acgtgttcgg cgcgcgagcc gcggcgcgcc tcgccgccgg cggaggcgag
    11521 cccgtcgacg ccgtgctgct cgccggcgac atcaccgaca gggggacggc cgaggaggca
    11581 cggctcttcg tgcgcgtgtt cggccgcgga gacccgcccg ttctcctcgt cggcggcaac
    11641 cacgaggacg agccggcgct ccgtgtcttt cgcgacgccg gctatcgcgt gctcgagggc
    11701 acgctcgagg acgtcgccgg cgtccccatc ctgggcgctt ctgatcccgt cgcgtcgcag
    11761 ccccgggtga gctcggacga ggagctcctc ggtgtctacg gagcgcgtct cgcgggtctc
    11821 tgggcggccg ccgacccgcg accgcaggtg ctgctcgtgc acgacgcccg gcaggcgcag
    11881 gacacgatca ccgcggccgc ggaagaggag gcagccctcg tggtcgccta cggcaacgac
    11941 cacgtggcgg gggtgcacgg cgaggacggg gtcgtcctgg tcgacgccgg cacggcgggc
    12001 gcctccgggt acgaggccat cggcgccgca tcgccgacgt ctgcgccggc ccgcgaaccg
    12061 ccggccgggt cacgctccgt ctacacgttc cagctcatcg acttctcccg ctccgcagag
    12121 ccgcgactca cgggggtgac gacggtgagc tacaccggaa cggggcggac cgtcgtcacg
    12181 tacacgccct tcgagccgtg agcccggcgg gacgggtcgc tgcctgccac cgcgccggcg
    12241 cgcggagggc ggcggagcct gccgtgcgcc tcatgtagaa tcgaaggccg cgcgccgccg
    12301 aaggcccgcg cgcgccggcc gcagcgagag aggactcacg tggacagacg gtacgacccg
    12361 cacgccatcg aggagaagtg gcagcgtctc tggaaggagc gcgacgccta ccgcacgccc
    12421 gacgagagcg acaagcccaa gttctacgcc ctcgacttct tcccgtaccc gtccggcgac
    12481 gggctcagcg tcggccactg ccgcaactac gtccccaccg acgcggtcgc gcggttcatg
    12541 cgcatgcgcg gctacaacgt cctccacccc atgggctggg acgccttcgg cctgccggcg
    12601 gagaactacg ccatcaagat gggcgtgcac ccgcgggtga cgaccgagcg caacatcgcc
    12661 aactaccacc ggcagatgga cctcatcggt ctctcgttcg actggtcgcg cgaggtcacc
    12721 agttgcttcc ccgactacta ccgctggacg cagtggttct tcctgctgct ctaccagcgc
    12781 gggctggcct accaggcgac gggacagcag tggtggtgcc ccagctgcaa gacgatcctc
    12841 gccaacgagc aggtggagca gggccgctgc tggcgctgcg gcagcctggt gaccaagaag
    12901 gacctcgagc agtggtactt ccgtatcacc gactacgcgc agcgcctgct ggacgacctg
    12961 gggaccatcg actggcccga gcagatcaag ctcatgcaga ccaactggat cgggcgcagc
    13021 gagggcgccg aggtcgactt cgggctgcag ggccgcgacg agaagctcac ggtgttcacg
    13081 acgcggcccg acacgctctt cggcgccacc tacatggtgc tggcgccgga gcacccgctg
    13141 gtcgacgcac tgaccgacga cgagcaccgg gcggcggtcg tggagtacca ggaccaggcc
    13201 cgccgccaga gtgagatcga gcgcatgagc acggagaagg agaagacggg cgtcttcacc
    13261 ggcgcgtacg cggtgaaccc cgtgaacgac gcgccggtcc ccatctggat cagcgactac
    13321 gtgctcatgg ggtacggcac cggcgccatc atggcggtgc cggcgcacga cgagcgcgac
    13381 ttcgccttcg ccacccggtt cgacatcccg atcgtcgagg tgatcgcgcc ggagtccggc
    13441 ccgcagggcg ccctgacgga ggcgtacacc ggcgacggcg tcatggtgaa ctcggcgcag
    13501 ttcgacggcc tgcaggcgcc gggcgaggcg ttcgaggccg tcgtcgcctg gctcggggag
    13561 cgcggcgccg cgcgcaagaa ggtcaacttc aagctccgtg actggctcat cagccgccag
    13621 cgctactggg gcgcgccgat ccccatcgtg cactgcgaga agtgcgggac ggtgccggtg
    13681 cccgacgacc agctgcccgt gctgctgccc gacctcgagg acttccaccc cacggacgac
    13741 ggccgctcgc cgctggcgcg cgccgaggag tgggtgcaca ccacgtgccc gggctgcggc
    13801 gggccggcgc accgcgagac cgacaccatg gacacgttcg cctgctcgag ctggtaccac
    13861 ttccgcttcg cctcgccgca cgacgaggcg gccgcgttcg accgcgagaa gacgcggtac
    13921 tggctgcccg tcgacctgta cgtcggcggc gccgagcacg cggtcatgca tctgctgtac
    13981 gcgcgcttct tctgcaaggt cgcgcaggac gcgggatacc tggacttcgg cgagccctac
    14041 gcgcgcctgc gcaaccaggg catgatccag gccgaggacg gccagaagat gagcaagagc
    14101 aagggcaacg tcgtcactcc ggacagtgtg gtcgagaagt acggcgccga cgccctgcgc
    14161 gtgtacgagc tgttcatcgc gccgttcgag cagagcgtcg cctggagcga ccgcggcgtg
    14221 cagggcacgt accgttttct ggggcgcttc tggaacctcg tcggcgaggt cgtggaggca
    14281 caggggagcg gggtggcggc cggcgaggcg gaggccgaga agggccgcca ggtcctgcgc
    14341 gcgctcaaca agaccatccg caaggtcacg cgcgacatgg acgcgttccg cttcaacacg
    14401 gccgtgtcgg cgctcatgga gctgcagaac caggtgctcg acgtgtggtc gtcggcgcgc
    14461 gggtcgctca cgctggcgca gtggcgcgag gtggtgcgcg acatgacgct gctggtgtgc
    14521 cccatggcgc cgcacgtggc ggcggaggtc tgggagctgc tcggccacgg cgacgacgtg
    14581 ctggacgcgc cctggcccgc gtgggacgag gagctcgccg ccgacgcggt ggtgaccgtc
    14641 gtggtgcagg tcaacggcaa gctgcgtgac cgcctcgagg tgcctgtgga cgcggagaag
    14701 gacgacgtgc tggcgatggc gcgcgcggcc gagaacgcgg cgcgcttcct cgacggcaag
    14761 caggtcgtca aggagatcta cgtgcccggc aagctcgtga acttcgtggt gcgttagaac
    14821 agcacgtcct gcagcttcgc gatcgccgcc gcctcgtggt cgccgacgcc cttgacggcg
    14881 tcggccgttg cctgcgcgac gccgagaagg aaccccttga acgccgtgag gtcggcgggc
    14941 gacttggccg cgacgacctc cacggcctga cgcacccgcg ccgtcacttc tccgcccatg
    15001 tcggccatgt cgtgggagac cgctttgtca cgtccgaact tgatgtcccc ggcaagatcg
    15061 tggacgagcg ggcttccgct ggacgccttg gcctgcgcca tgaacttcgc cgccgcgctg
    15121 gcctccttga aggtgtccca gaagccggcg tccgccatcg agacgtaggc catcgtgtcg
    15181 gtcaccgccc actgcagcgt gtgccattcg tcgtcggtga acatcgcctt gctggtcatg
    15241 ccgcctcccg ggtgtcgtgt gccgtcgcgc ccacctccga ccctatccca gcgcggtcac
    15301 cccgtgaaat cgcccctgag gggtgatgcg gggcgcggga cgctgccgta gcctgcgacg
    15361 cgacgggcgc cggacgcgcc cccggcgccc gtcacttccc tcgcagcgac cgcggcgccg
    15421 gaggtgggcc gtgcagctct cgcgacgcca gatcatcgcc tacgtcgccg tcgccgcggt
    15481 ggtcgtcgcg gtcggcgtcc gctacgtcgt gctgccgcgg caggccggcc cgccgcaggc
    15541 gcaggcggtc gtgctcgcgc cggtcgccgc gtcgccggcg agccaggacg cgggagcgtc
    15601 ggccggggcc acggcggcct cgccggcgcc ggacatgatg gtgtacgtgt gcggcgccgt
    15661 gcgcgcgccc ggcgtcgtcc ggctcccgcc gggggctcgg gtgaccgacg cgctccaggt
    15721 ggccggcggc cccacggcca aggccgagct cgcggcggtc aacctggcgg ccccggtctc
    15781 ggacggccag cagatcgtcg tccccgagcg cggggcggcg gtcgtggcgg cgcaggcgtc
    15841 cggcgggtcg tccaccggcg gagcgccggc gctcgcccct gcggccgccg ccggcgggct
    15901 gatcaacatc aacaccgcct cgctggagga actggacgcg ctcgatgggg tcggcccgtc
    15961 cacggcgcag aagatcatcg actaccgcac ggcgaacggc ggcttcacga cgatcgagga
    16021 gatcaaggag gtcccgggca tcggcgacgc caagttcgcg gcgatgcagg acgccatcac
    16081 cgtctgaggc cgcgacgtgc gcctgccggc tcctccgcac gtgctgctcg gcgcctactg
    16141 tctggggctc tgcctcgtcc tggcgtggcg gccgccgccg gagctcctgg cagcggccgc
    16201 cgcgctggcc gccggcgcgc tcatcgtcgg cgtccgcgcg ccggccctct cgggtcgcgg
    16261 cccggtggcc ggtctcgccg ggctggccgc gctgttcctc tgcgccggag ccgtgctcgg
    16321 cggcgcccgc ctcgacgccg tcgcccggtc ctcgctcgcg gcgtacacgg gcgaccgcgt
    16381 ctcgctggcg gcggtcctca cggacctgcc ggcgatcgac gccgaccagg tgaccctggc
    16441 cctgcgggtg accgacgtcg ggggcaggcg actgcgcgag ccggcacacc tgcgcctgcg
    16501 actcgaggac gacaaagcct ttgcgcacga ccccaccggg cccctcacgg agggcgcgct
    16561 gctgcaggtg gcgtccgtgc gcgtcgcgcc gctcccggag ccgcgtcccg gcgccttcga
    16621 ctacggccgc taccttcggc ggcgtggcga gcacgtgctg ctcgagggcg acttcgccga
    16681 tctggtggtg accggccgcc gtgaggggct gcagggcatg gtggaccgcc ttcgtctggc
    16741 gtcgcgcgcc catctcgccg ccggggtgca ctctccggtc gccgacgtgc tgcagggcat
    16801 ggtcctcggc gacgacgagg gggtggacgc ggcgctcgtc gaggacttcc gcgccagcgg
    16861 cctgctgcac atcatggccg tctccgggga gaacgtggtg ctgctctgca ccatgtggtc
    16921 gttcgccttc gcgctgctcg gcatgcaccg gctcctgcgt accgcgctgc tgttgcccgt
    16981 cgtggcaacg tacgtgctgc tcacgggggc gtcgccgtcg atcgtgcggg ccggcgtcgc
    17041 cggcatctgc gggctgctcg ccatcctcgc ctcgcgcccg agcgacggct ggctgctctg
    17101 gctcgccccg gcggcgtggc tgctcacggc caatcccaac aacctgttcg acgtgagctt
    17161 ccagctgtcg ttcggcgcgg tcgtcgggct gctggtgctc gccgggccgc tgacccgtgc
    17221 cgtctccttc ctgccggggc cgctgccgga gcaggtgggc gtcacgacgg ccgccagcat
    17281 ctccaccgca ccggtgtcga tgctgacgtt cggctccgcc tcgctcgtca gcgtgcctgc
    17341 caacgtggcc ggggggttcg tgctgggccc gatcatgttc ctcggcatgc tctcgctcct
    17401 cctggggttc gtgagcgagt ggctgtcggc gccgctcaac gtcgtggccg ggctgttcat
    17461 cgggtttctg ctcgccgtgt cgcacctctt cgccgggctg ccgggggcag tctacgagta
    17521 ccaaggcgtc tcgctgcggc tgctgctggt cctcgggctg ctcgccgaga tcgcgctggt
    17581 cggggcgctc gcggcgcggg ccggtgaggg cgtgctcgcc tacgtgcgcg accgccgccg
    17641 atgggcgccg ctcgccggcg cgacggcggc gctgctcgcg gcggcgctgc tcctggcgcc
    17701 cgcgccggtg cggccgccgg atgtgccgac gttgacgttc ctcgacgtcg gcgagggggc
    17761 ggccactctc cttcaggtgc ccggcggccc aacggtgctg gtggacgctg gccccgaggc
    17821 tctggcgcgg acgctgcggg agcacgccgt cggggaggtc gacctgctcg tgctctccca
    17881 cgggcacgac gaccacgtcg gcggtctcgc ggacgtcatc ggcgccgtca cgatccgggc
    17941 ggcgctgctg ccgcagccgg aggagccgtc cgctgccctc gacgacctcg aggaccgcct
    18001 gcgcgccgcg ggcacggacg tgcgcaggtg cacgacggac ctgcggctgg gcggagccgg
    18061 gtggagcctc gacgtcctgc cgagcgcccc ggtggggggc gtcgaccaga accagaggga
    18121 gaacgacgac gcgctggtgg cggtggtggg gctggcgggc cagcgcgtgc tcctgccggg
    18181 cgacgcggag gggcaggtgc tcgagaacct ggacctgccg ccctgcctcg tggtcggcgt
    18241 gccgcatcac gggagcgacg gcgggttcga cgccgggttc cttcggcagc tcgcgcctcg
    18301 cctcgcggtg gtccccgtcg gccccaaccg gcacgggcac ccggaccagg acgccctggc
    18361 ggtgctgacg gccgccgggg tgccctacgc gcgcacggac cgatccggcg acatcgccct
    18421 ctcggcgggg agcgagggtc tcagcgtgac cgcggagcgc gaaggttgag gacgctcaga
    18481 tctcgcggcg ctcgaagcgc agcacggcgg ccaccaggca cgccgcggcg acggccacag
    18541 tcgtcacgag cggcagcgcc cactgcgcgg gctcgccacg cacgatcgcg agtcccgctg
    18601 cggggagccc agccggggtg gcgccgctgg tggcctcgaa ctgcgcgagc acgaccaggg
    18661 ccgcgtacac gccgatgccg gcgccggagg ccgccgccgg agctttgagc tcgacgctga
    18721 gcagcaccat gaccgccagc agcatgagcg cgtaggcgat ccagaggccc acggcgagaa
    18781 cggtcctgcc ggccgggccg tcctcgaaca gcagctgcgt cagggcgatg cagatgagcg
    18841 tcccgaacgc cgcaccggcg gcgaccacca gcgcctgaga gagccacttg ccggtcacga
    18901 acgcggcgcg ggagagcggc ttggccagcg tgagcgccgc cgtgcctccg cgcacctctc
    18961 cgctcacgat cccgccgtag gcgatgacga gtgcgaacaa cgtgagctgg ccgaggttgc
    19021 cgaggtactc gaggtaggcc tgcagggcgg tggggtcggc gacggtgatg ctgagcccct
    19081 gctgcgccgt gccgagctgg tcgagcagcg tcggcatgag gtaggtgacc agcggcgagc
    19141 tcagggcgga gaagagcagg aaccccggca cgacccagag gcgccaggtg cgcacggtct
    19201 cgctgaactc cttgcgcagg aaggggacga ggccgctcat cggcggtcct ccccgacgag
    19261 gtcgacgaag acgtcctcga ggtcgagctc gtcgacgccg aagcgccgca gcgcgaggcc
    19321 cagccggccc acgagggccg gcagctcgcg ctgcgccgcc ccgacgtcgt cgcaggtcac
    19381 gaggagctcg ccgctctcgc ccgcctccac cgtccgcagc cacggggcgc ccgcgagtcc
    19441 ctcccagagg cgccgtgggt cgtcgacctc gatgctgagg cggttcagcc cgcccctgcg
    19501 gctgcgcagc tcgtcgatgc cggactgcgc gacgacgcgg ccgcgggcga tcacggcgac
    19561 ggtgtcgcag acgcgctcga cgtcgctgag gatgtgcgtg gagaagaaga ccgtcgtgcg
    19621 cccgcgcatg gaggcgatca tgtcgagcac gtcgcgccgg ccgatcggat cgagcgcgct
    19681 ggtcggctcg tcgagcagca gcaggcgcgg cccgtggatg agcgcctggg cgacaccgag
    19741 ccgctgcttc atgccgcgcg agaagccccc gacccgcgtc cgcaccccgg ccagcccggc
    19801 gagctcgagc atggcggtca cgcggtcgtc cagctcccgg ccggaaaggc cgaacaggct
    19861 gccggcgaag cgcaggtact cgggcgccgt catccacttg tagaaggccg ggacgtcggg
    19921 gaggaagccg atgacggcgc gcgcgtcggc gccgccgccc gccacgtcgc gaccgaggat
    19981 gcggaccgcg ccggcgtcgg cgtgcgcgag gccggccagg atgcgcaagg tcgtcgtctt
    20041 gccggccccg ttggggccga ggaacccgta gatggagccg ggcggcacgc tgagcgtgag
    20101 gccgtccagc gcgaccgcgt cgccgaaggt cttgcgcacg tcgcgcagct ccacggccgg
    20161 gccggaggcg gctgcgccgt ccggctccgc gccgcgcgcc gcgaagagcg cgtcggccac
    20221 cgtcgcggcg tcggcgcgct ggaccgccag cggcggggga acggcgccgg cggccgcaac
    20281 gcccgcgacc gtagcgccgc cggccacagc gcgtccggcg gcgctccccg cgggtccgcc
    20341 gccggccagg gggtcgtgcg ccggcttcac ccgctcccgc cccgccgcga ggtagacgat
    20401 gggcccgatg atctgcagca gcaggcagag gatcagccag gggatcttgc tgccgccgtt
    20461 gatctgcgcc gacggccgcc ggtacaggtc cacgagcgcc caggccagca gtcccacctc
    20521 gacggcgccg aggacgacga gcagcgcgac gagcgccggc gacgtgccgg tcgccgcgac
    20581 gtgtgtcgcg atgggcagcg ttgtctcgac caatgtcacc cctgatccgt ggacggcggc
    20641 gccggccgtg agcatcctat cgccatgcta tcctcacggc accatggccg agcccacccc
    20701 gctgcttccc gcctacctca tcgtcggcac cgaccgcccc aaggtgcgac gcgccgtcgt
    20761 gcgcctgcgg cagcgcgtga tcgacgaggc cggcagcgac ctcaacgtgg tgctgctcga
    20821 cgccgagcac gacccggtcg aggcgttcct cgatgcggcg gtcagccccg gcctcacgtt
    20881 cgggacccgc ctgctcctcg tcctcaacgg ccacaaatgg aaggcaaagg cgcggcaggc
    20941 cgtggtcgcc tacctgcagg accccatgcc cgacacgtgc gtcacggtcg agggcgagac
    21001 gttcgccgcc tcggacgcgc tgtacaaggc ggtgagcaag ctcggcggcg tgctgcgctt
    21061 cgacctgccc aagaagtacg agatggcgaa gtgggtgagg gaacgggcca aagctcaccg
    21121 tctgccgctc agccagatcg tcgccaagca cctgctcgac cggtgcggca ccgatcccag
    21181 gcacgccgac cggctcgagc gtgagatcga gaagctcgcg ctgtactgcc gcggccagga
    21241 ggccaccgag gccgacgtgg acgcgatctg cacgccggac gacgacgcgg tgatcttcga
    21301 cctcatggac gccgtggggc accgcgaccg cacccacggc ttcgcgttgc tcgaggccct
    21361 ctacgcttca ggcaacccgc gcaacgacgc caacggcgtg ctgtacagcc tcaagcggca
    21421 catcgagaag ctcgacgcgg cgtcccagct gccgcacgcc gaccagacga cggccgcgaa
    21481 gcagctcggc gtgcacccct tcacggccaa gaagctgctg gagcagcgag agcacttcga
    21541 ccggcgccgc ctgggccgcg cgtaccgcgc gctcgccgag gccgaggctg ggttgcgcgg
    21601 ccgggcgccg gcgacgctcg agtcgatcgg cggcgtcaac gacggcgagc gcctcgtggt
    21661 cgagctggcg ctggcgcggc tgctgacgta ggggagaggc cggcccggct gcggcggccg
    21721 gccctgccgc tgaacgggga cgacgaagcg ggcgggccgc tccaggcggc ccgcccgctt
    21781 cgtcgtcgtt ctcagccgcg aaccgtcagg aagagaggcg cgccatggtg tgcgcgacgc
    21841 ggctcttctt gtgggcggcg ttgttcttgt gaaggatccc cttggcggcg gccttgtcga
    21901 tgcgcgaggt caggaaggtc gcggtcttcg ccgcttcctc ggtctggccc tcggcgacct
    21961 cgtcctcgag ctgcttgaac agcgtcttga gggtcgactt ggcgtgccgg ttgcgcagcc
    22021 gctgcttggc tgccaggcgg atacgcttct tctgctgctt gatgttggcc acgtgatccc
    22081 ttcgatctgg ctagattcgt gacggttcca ggtgcagtgg ggctgcgtcc ggacgcgcga
    22141 tgctatcacg gcgcgtcagc gcgcggcaaa cgtcggcggg gtcaggcaga gggtcggagc
    22201 ccggctccgc tcagcgcagc tcgcgcgtga tgaggggcca gaagccggag gcctcctggc
    22261 ccatcaccca ggcgctgatg ccggcgatcc cggggtggcc cgcgcgcagg gctgcgagct
    22321 tggcggcgac ggccgcctcg tcctggaagt aggccgtgtg ggtgaccccg ccgtcggtga
    22381 aggtcatggt cgcctcgtct gaggaggggt cgcggcccac ggtggcgtcg tgggtctgcg
    22441 ccagcgacgc gccgcggtgc gcggagacgg tgtcgcagga gtcgccgtac cagtcgaagc
    22501 cgtagaaggg gatgcccatc gagatcttgc gcggcggcac tttgcgctcg gcgaacgtga
    22561 gcacgcggtc cacccactca gccggcgcct gcgggccggg gtcgccccag gggccgctgt
    22621 agctgtaggt catgatcttg aactcgtcca cggccttgcc gatccgcttc cagtcggcgt
    22681 acttctggtt gtcccactcg ccgggctcgg acgtcttggg catgacggcg atggagagga
    22741 agcgctgctc ggcgtgcagg gcctcggcga gctcctccac gagcaccgag aagctgtccc
    22801 ggtcggccgg cctgagctcc tcccagtcga ggtcgacgcc gtcgtagccc atgtcctcga
    22861 ccagggtgag caggtcgtcg atgaagcggc ggcggcggtc gggcgaagcc agcaccgccg
    22921 ccgcgacgtc acggctgaac gcgctgcccg agctggtcga gttggtcacg gtggcgaaga
    22981 tgttcaggcc cttgtcgcgg gccgctccga cgagagcggg atcctcgttc tcggcggtga
    23041 cgctgccgtc cgcctgcgtg tggtaccagt cgaagtcgat ctcgtcgacg gcgttcgcgg
    23101 cggtcgccga agtcagcgag gcggcgtcgc cgagcgtcca cgccgtcacg cggtgctgcc
    23161 gcccgccgag gggctcgatg aggtagccga ggggagggtc accgggtcgg tcgaggtgcg
    23221 acccgaacgg cccgaccgtg aagagcaaca cgaccaccac ggccagagcg ccggcggcga
    23281 gcagcaggac cagcagtcgc tgccggcgca gccgcgcggc cctcacctca cgggacgcgc
    23341 gcgtcacgcg acgatgatcg ctcctctgtg gagcctctcc tgcgtcgttg ggcgtcggtg
    23401 cagggacgct gcttcactcc tctgaacgcg ttgcgtgcgg acatgacaag cacgaccttg
    23461 gccggaccaa ggctaggtgc aggctcgcgg gaggtcaact gctccccctc cggacaggca
    23521 cgcttgcgcg gcggcgctct ggcccctgcg cgagtgcggt ggtacgttcc acgccatgac
    23581 cggtctccgg cagacactgg caacgctcgg tctccttgcc ggcgcgaccg tggcgctcgc
    23641 gacgggcggg tgcgcctcgg agcacgtcgc gcaggcggcc ctggagccga cgtccgaggc
    23701 cgcgctcgct tccgcgtcgc cgtcgtcctc gccgtcgccg agcccgagcg cgccgtcgga
    23761 gctcgccggg tggggcaacc gccggctggc cgcgcaactc gtcgtgtgca gcgtgcccgc
    23821 gacgaacccg ctctccggat gccggtacgc gcgcatgggc gtcggcggca tcgtcatcct
    23881 cggcggcggg gcgcgcagca gcatcggcgg cgacctcgcc gccgtcctgc gcgccgcgcg
    23941 cggcggcgtc aagcccctca tcgccagcga cgaggagggc gggcaggtgc agcggctgcg
    24001 ctacgtcatc tacccgttgc catcgccccg tgtgcagggc gggtggagcg ccgcgaagac
    24061 cagggcgacc gcgaaggact acggagcgcg gcttcgcaag ctgcacgtct cggtggtgtt
    24121 cgggccggtg gcggacctgg acatcgaggg ccgctacatg agctccctcg accggtgctt
    24181 ctcgcctcgg ccggacgtcg tcgggagtca cgtcctggcg tgggtgagcg gcctccgggc
    24241 gtcgcgggtc ctcgcgaccg tcaagcactg gcccggtcac ggctgggcca ccgacacgca
    24301 cagcggcgcg gcgcgcgtcc cctcgctctc ggtgctgcgg cgctacgaca tggtgccgtt
    24361 cgagaaggcg ttcgacgccg gtgtgccgct ggtgatggtg ggccacctgc ggtccaaggg
    24421 gctcaccggg gacgagacgc cggccagcct ctcgcggacg gcgatgacct acctgcgcgg
    24481 ccgcatcagc gacaagaccg tcatcatcac cgactcgctg tccatggccg cgacgaccaa
    24541 ggccgtgggc ctcaggttgc cggcggcggc ggtgcgcgcg ctcaaggccg gcgccgacct
    24601 cgcgctgttc gccacgggcg acccggggcc ggtgatcgac gccgtgacgg cggcgatcaa
    24661 gtccgggcgc atccccaggg ccgaggcgga ggcgaaggtg ctgcgcgtcc tggaactcaa
    24721 gcgcagtgcg ggtctggcgc cggcggcgca gtaggcgcag gccggcgccg gctgtacacc
    24781 ggtaagggcg gccgggccgg gaggcgggcc cgccctctgc tagactctcg ccggcctcgc
    24841 cgggcagtac ttcgccgccg gcggtcgaat caagggactc atgagcgatc acaagcgccg
    24901 gctcgccctc gcggccgtca tcgtcgcctc cgccaccgcc gtctcgcgcc tcaccgggct
    24961 cgggcgggag gtcatcaccg ccgccgtcta cggcgtcgac gcgtcctaca acaccttcgt
    25021 ctcggtctcc gtgatcccgc aactcgtgca gcagttgttc gccgacgccg cgatcagcgc
    25081 cgcgttcgtg cccgtgttca cggcgctgct ggcaaaagga gagcgagacc gcgcctaccg
    25141 gctcgccgcc aacctgctcg ggctgatcgt ggtggtcgtg ggggcggccg tcgtgatcct
    25201 ggcgctggcc gccggcccgc tcacgcagtg gatgtacccg gagctcaccg gcacgcccgc
    25261 cggcgcggac ctcgccgccg acctcctgcg catcctcgtg ccgacgatcc tgttcctctc
    25321 gctggccggc gccatcagcg gcgtcctcta ctccttcgag cgcttcacca tgccggccgt
    25381 ggtgtccatc gtctggaacc tgacgatcat cgccgccatc gccatcttca acgacacgct
    25441 cggcgtggac gcgatcgcct ggggcatgct cgtcggcacc gtcctcgagg tcgtgatgct
    25501 ggccggcgcc gcccgctacc acgggcgctg gctgtggccc ggcttcggcc tcaccgaccc
    25561 gctgctgcgc cgcgtgctgc tgctcatggt gccgatcacg atcacgctcg gcgtgctcaa
    25621 cttcaacgcc ctgatcggca cgtggttcgc ccagttcgtg agcgaccgcg cggcggccga
    25681 gatcggctac gcgttccgcc tctaccagct gccgcagggc atcttcgcgg tgaccatcgg
    25741 caccgtcctg ttcccgtcgc tctcgcggtt cgcggcgcag aaggacgaga cccggttccg
    25801 cgacaccgtc tcgatgggtg tgcgccagat ggtcttcgtc tcattgccgt tcgtcgcctg
    25861 gttcctggtg atgccggccg cgttcgtcca gctcgtgtac gagcgggggc agttcggcgc
    25921 ggacgcgacg a
//
LOCUS       DASUMX010000214         2718 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_796252, whole genome shotgun sequence.
ACCESSION   DASUMX010000214 DASUMX010000000
VERSION     DASUMX010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_796252"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="VFZ86_10325"
     CDS             complement(<1..489)
                     /locus_tag="VFZ86_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013164436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HEX5642713.1"
                     /translation="MSTTLTDEAIIAAAGRVAEPPRPVPFAFAKRHGVLVRALTEQSA
                     DVVLRAGASPIALAEVRRHLRRPLTVERADADRFEMLLRQAYEGGSSAAMDAMGGLEE
                     DTDLGSLAQEIPEPSDLLESEDEAPVIRLINALLTQAVKENASDIHIEPFENRLVIRF
                     RVD"
     gene            complement(486..2684)
                     /gene="gspD"
                     /locus_tag="VFZ86_10330"
     CDS             complement(486..2684)
                     /gene="gspD"
                     /locus_tag="VFZ86_10330"
                     /inference="COORDINATES: protein motif:HMM:TIGR02517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HEX5642714.1"
                     /translation="MKSKFKDRVAVKLAAGALAAALALGMPAAAPGQSQGSITPNYKD
                     ADLGQIIEAVSAVTGKNFIVDPRVRAQVTMLSSTPMSPDAFYEAFLSILQVHGFVAVQ
                     SGDVVKIVPDANARQLPANDLPSRISSTSDEIVTQVVSVKNVSAAQLVPILRPLIPQY
                     GHLAAYPTSNMLIISDRAANVSRMVRIIQRIDQTGDENIDVIPMQHASASDVVRIVNS
                     LYVTAGAEGGGVPTVKMVADDRTNSVLVSGESSQRLRLKTLITHLDTPLEAGGDTQVR
                     YLRYADAEKISQKLREQIQGITAAAAPGAAGPGGGAPAIVSGGGGGVDRSITIWAEPQ
                     TNALVVTAPPKVMRSVMSIVDRLDIRRAQVLVEAILVEMSFDKSMDLGINWLIGDRDS
                     NGNLLPAGGFVQPVDGTGIGQILQGVLNPESLAGLPSGLTMGVGQIVEGGFSWAALIR
                     AIGGQGNTNIIATPSIVTLDNEEAEIKIAQEVPFVTGQYTNQGVSTPGGNVNPFQTIN
                     REEVGNILKITPQINEGDSVVLKISQEASSIAASSQQVSTNDLITNKRTISTNVLVED
                     GGIIVLGGLISDEVRESKSQVPFLGSIPILGELFKTRSVDKVKTNLMVFIRPRILRDG
                     VDAAIETNAKYNYIRNQQLERNDGKIPLMPGERQPTLPALEALVPPELLNPARQASPM
                     SDTPAERAIATDGTPLPLPPPAAAPAQPVTPTTPFGNEPVAPPPGDRPPP"
BASE COUNT          363 a          888 c          989 g          478 t
ORIGIN      
        1 gtccacccgg aagcggatca ccagccggtt ctcgaacggc tcgatgtgga tgtcggaggc
       61 gttctccttc acggcctggg taaggagcgc gttgatcagg cggatgaccg gcgcctcgtc
      121 ctcgctctcg agcaggtcgg agggctccgg gatttcctgt gcgaggctgc cgaggtcggt
      181 gtcctcctcg aggccgccca tggcgtccat ggcggcgctc gagccgccct cgtaggcctg
      241 gcgcagcagc atctcgaagc gatccgcgtc cgcgcgctcg acggtgagcg ggcggcgcag
      301 gtggcgtcgc acctcggcga gggcgatcgg ggaggcgccg gctcgcagca cgacgtccgc
      361 cgactgctct gtgagcgcgc gcaccagcac gccgtggcgc tttgcaaagg cgaagggcac
      421 gggccgcggc ggctcggcga cccggcccgc cgcagcgatg atggcctcgt cggtgagggt
      481 ggtgctcacg gcggcggccg gtcgccgggc ggcggcgcga ccggctcatt cccgaacggc
      541 gtcgtcgggg tcaccggttg cgccggggcc gcggcgggtg gcgggagcgg cagcggggta
      601 ccgtccgtcg cgatcgcacg ctcggccggc gtgtcgctca tcggcgaggc ctgccgggcc
      661 gggttcagca gctccggggg caccagcgcc tcgagcgcgg gaagggtcgg ctgccgctcg
      721 cccggcatca gcgggatctt gccgtcgttc cgctcgagct gctggttacg gatgtagttg
      781 tacttcgcat tggtctcgat ggccgcgtcc acgccgtcgc gcaggatgcg cggacggatg
      841 aacaccatca ggttggtctt gaccttgtcc acgctgcgcg tcttgaacag ctcgccgagg
      901 atcgggatgg acccgaggaa cggcacctgc gatttcgact cgcgcacttc gtccgagatc
      961 aggccgccga gcacgatgat gccgccgtcc tcgaccagca cgttggtcga gatggtgcgc
     1021 ttgttcgtga tcaggtcgtt ggtgctgacc tgctgcgagc tggcggcgat gctcgaggcc
     1081 tcctgcgaga tcttgaggac caccgaatcg ccctcgttga tctggggcgt gatcttgagg
     1141 atgttgccga cttcctcgcg gttgatcgtc tggaacgggt tgacgttgcc gcccggcgtc
     1201 gacacgccct ggttcgtgta ctggcccgtg acgaacggca cttcctgggc gatcttgatc
     1261 tcggcttcct cgttgtcgag cgtgacgatc gagggcgtgg cgatgatgtt ggtgttgccc
     1321 tggccgccga tcgcgcggat cagcgcggcc cagctgaagc cgccctcgac gatctggccg
     1381 acgcccatgg tcaggccgct cggcaggccc gcgaggctct cggggttcag gacgccctgc
     1441 aggatctggc cgatgccagt gccgtccacc ggctgcacga agccgccggc gggcagcagg
     1501 ttgccgttgc tgtcgcgatc cccgatcagc cagttgatgc cgaggtccat ggacttgtcg
     1561 aaggacatct cgaccaggat ggcctcgacc agcacctggg cgcgacggat gtcgaggcgg
     1621 tccacgatgg acatcaccga gcgcatgacc ttgggcggtg cggtcacgac gagtgcgttg
     1681 gtctgcggct ccgcccagat cgtgatgctg cggtccaccc cgccgccgcc gccgctgacg
     1741 atggccggtg cgccgccgcc gggacccgcc gcgccgggcg ccgcggccgc cgtgatgccc
     1801 tggatctgct cgcgcagctt ctgcgagatc ttctcggcgt ccgcgtagcg caagtagcgc
     1861 acctgcgtgt cgccgcccgc ctcgagcggc gtgtcgaggt gcgtgatgag cgtcttcagg
     1921 cgcaggcgct gagagctctc gccgctcacc agcacgctgt tggtgcggtc gtcggcgacc
     1981 atcttcaccg tcggcacgcc gccaccctcg gcgcccgcgg tcacgtacag cgaattgacg
     2041 atgcgcacca cgtccgaggc gctcgcgtgc tgcatcggga tgacgtcgat gttctcgtcg
     2101 ccggtctggt cgatgcgctg gatgatgcgc accatgcggc tcacgttcgc cgcacggtcc
     2161 gagatgatca gcatgttgga ggtcgggtag gccgcgaggt gcccgtactg ggggatgagc
     2221 ggcctcagga tcggcacgag ctgggcggcg ctcacgttct tgaccgacac cacctgcgtg
     2281 acgatctcgt ccgaagtgga gctgatgcgc gagggcaggt cgttggcggg cagctggcgg
     2341 gcgttggcgt cgggcacgat cttgacgacg tccccggact gcacggccac gaagccgtgc
     2401 acctgcagga tggacaggaa cgcctcgtag aaggcgtcgg gggacatcgg cgtggaggaa
     2461 agcatcgtga cctgcgcccg cacccggggg tccacgatga agttcttgcc ggtcacggcg
     2521 ctcacggcct cgatgatctg gccgaggtcc gcgtccttgt aattgggggt gatggatccc
     2581 tggctctggc ccggtgccgc cgcgggcatg ccgagcgcca gggctgcggc gagtgcgccg
     2641 gcggccagct tcactgcgac ccgatccttg aacttggatt tcatcgtcac tccgcgggat
     2701 ccgggtcttc ggaggcct
//
LOCUS       DASUMX010000215         4317 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_796257, whole genome shotgun sequence.
ACCESSION   DASUMX010000215 DASUMX010000000
VERSION     DASUMX010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4317
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_796257"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="VFZ86_10335"
     CDS             <1..429
                     /locus_tag="VFZ86_10335"
                     /inference="COORDINATES: protein motif:HMM:NF018184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HEX5642715.1"
                     /translation="ASQLALTRRERVTMKTIGVGLDAGFGKLRQHVAATGAETVGPPF
                     VFSPDFGKDEFDLVICMPVTDGAVAGEGIAVEEIPGGHVVSTTHKGPYTEVPEGYGAL
                     FKWVEEHGRKPAGAARETYLNDPKDVPESELLVEIAIPLA"
     gene            683..1054
                     /locus_tag="VFZ86_10340"
     CDS             683..1054
                     /locus_tag="VFZ86_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642716.1"
                     /translation="MSGLRRILLYGDSVVLGSVGASLGRAAGFELVRLRAARPTAAEL
                     RAARPDVILFDIENDHPRAAFRLLEDDPDLLLLGISPDVNLVRLWEGRQYRELTADAL
                     ATLIAGGAGQRSTSDGKEGAA"
     gene            1051..2205
                     /locus_tag="VFZ86_10345"
     CDS             1051..2205
                     /locus_tag="VFZ86_10345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642717.1"
                     /translation="MRDHTCGGSTTAEGRSGRRAGSLFLLAALVGAFILFLVATPRAD
                     AFVYWTNSHGDSIGRANNDGTGVNQTFIMFVGHPKNIIVDDYHIYWSTWDTIGRAGLD
                     GTNANVNFITGCQPRGLAIYNDHIYWADYSGYVGRATTSGTGIDRTWIDCKAIPDNLT
                     QHAGYFYWSWRRAGADDPRIARCNPPTAAGGTVNYTNILGDYWVRPIQGICATASHLY
                     WRSDGEIGRASTPYGLSPDRNYITGLAGAGGMTVYDDHIYYTTIGSFTYPEGIGRVGT
                     GGGGLNNALITGCSEPMDVDVDGHSAPTSLLGVARDISAAGLPTRLATPLVRKLRSAK
                     AALDRDQPKVALNIIDATIGLIRAQSGKHTPSEQADRWIQVLGLIKVHII"
     gene            2246..3388
                     /locus_tag="VFZ86_10350"
     CDS             2246..3388
                     /locus_tag="VFZ86_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642718.1"
                     /translation="MDTTLRAPARARHGARMRGLVLLGLLVSSFALLLALTPRAEAFV
                     YWTNQTGYSIGRANNNATGVNQNFITGLDRVFDVYVDDWHIWWTGWNSIGRASLDGTN
                     VNNSFITGITPTGLTVYDHHVYWSDYSGYIGRAATDGTGIDRTWINCKAIPYNVTQHL
                     GYFYWNWSDSYGNDYIARDNPTADGGTVSHMDVLGEYWIEFPYGLCATASHLWWRTDD
                     AIGRASTPYGLSPDRNYITGLEGYGSGLAEYNDYLYYTTSGFWEGPANIGRVKNDGSG
                     LNNAHITGCSTPSGVFVNGGSAATSVVALAGQIDAAEPTAGRLPSLVKKLTNAKAALD
                     RGNVKTARNIVDAAINEIEAQSGKMIPAETAERWITALTLIRVHIV"
     gene            3834..4202
                     /locus_tag="VFZ86_10355"
     CDS             3834..4202
                     /locus_tag="VFZ86_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642719.1"
                     /translation="MGERRILLYGDSVVLGSVGASLGRTPQFELIHLETPRASAAELE
                     AFRPDVILFDIENGHPEAAFCLLESDPDLLLLGISPDVNLVRLWTGRQYSELSADALA
                     ALAAGGTGQAPKSPRKDGES"
BASE COUNT          777 a         1495 c         1406 g          639 t
ORIGIN      
        1 gcctcgcagc tcgcgctcac gcgccgcgag cgggtgacga tgaagaccat cggcgtggga
       61 ctggacgccg ggttcgggaa gctcaggcag cacgtcgccg cgaccggcgc cgagacggtc
      121 gggccgccct tcgtcttctc gccggacttc ggcaaggacg agtttgacct ggtcatctgc
      181 atgcccgtga cggacggcgc cgtcgccggc gagggcatcg ccgtcgagga gatccccggc
      241 ggtcacgtcg tgtccacgac ccacaagggc ccctacacgg aggtccccga gggctacgga
      301 gccctgttca agtgggtcga ggagcacggt cgcaaacccg ccggcgccgc ccgcgagacc
      361 tacctgaacg accccaagga tgtccccgag agcgagctgc tcgtcgagat cgcgataccg
      421 ctcgcctgag cacgggttgc gctgagggcg gcgagccgag aagcaggcga cgactgtcta
      481 cgtccgcgct caccccgacg acccgatctt cctgagcgac ttctccacct ccttgagcct
      541 gaggacctcc gggccgccat acgccgtgca catggcatga ctgtcccgcg actgtccccc
      601 cgactgtccc gcgggacatt gtgcgccggc cccgcctgct cgagagtcag gcaggtcggt
      661 gggacctgcg aaaggcacgg gcatgagcgg actgcggcgc atccttctct acggcgattc
      721 ggtcgtcctc ggcagcgtgg gcgccagcct cggccgagct gccgggttcg agctggtccg
      781 cctgcgcgca gcgcggccga ccgccgccga gctgcgggcg gcccggccgg acgtcatcct
      841 cttcgacatc gagaacgacc atccgcgggc ggcgttccgc ctgctcgagg acgacccgga
      901 cctgctgctg ctcggcatca gccccgacgt gaacctggtg cgactctggg agggccgcca
      961 gtaccgcgaa ctcactgccg atgccctggc gacgctcatc gccggcggcg ccgggcagcg
     1021 atccacatct gacggaaagg aaggcgcggc atgagagacc acacgtgcgg aggcagtacc
     1081 accgcggagg ggcgcagcgg aaggcgcgcg ggcagcttgt tcctgctcgc cgctctcgtc
     1141 ggcgccttca tcctgttcct ggtggccacg cccagggcgg atgccttcgt ctactggacc
     1201 aactcccacg gagacagcat cggccgcgcc aacaacgacg gcaccggagt caaccagacg
     1261 ttcatcatgt tcgtcgggca cccgaagaac atcatcgtcg acgactacca catctactgg
     1321 tcgacctggg acaccatcgg tcgcgccggc ctcgacggca ccaacgcgaa cgtcaacttc
     1381 atcaccggct gccagccccg gggtctcgcg atctacaacg accacatcta ttgggccgac
     1441 tactcgggct acgtcggtcg ggcgacgacg agcggcacgg gcatcgatcg cacctggatc
     1501 gactgcaagg cgataccaga caacctgacc cagcacgcgg gctacttcta ctggtcgtgg
     1561 cgtcgcgccg gggcggacga tccgcgcatc gcccgatgca accctccgac cgccgccggc
     1621 ggcaccgtca actacaccaa catcctgggc gactactggg tccgccccat ccagggcatc
     1681 tgtgccaccg caagtcacct gtactggagg tccgacggcg agatcggacg cgcgagcacg
     1741 ccctacggcc tcagcccgga ccgcaactac atcaccgggc ttgccggcgc cggtgggatg
     1801 acggtgtacg acgaccatat ctactacacc acgatcggct ccttcacgta tccggagggc
     1861 atcggtcgcg tgggcacggg cggcggaggc ctgaacaacg ccctcatcac cggctgttcg
     1921 gaacctatgg acgtggacgt ggacggtcac agcgcgccga cgagcctgct cggcgtcgcc
     1981 cgcgacatct cggcggccgg cctccctacc cgcctggcca cgccgctggt gcggaagctc
     2041 aggagcgcca aggcggccct ggacagggac cagccgaagg tcgcgctcaa catcatcgat
     2101 gcgacgatcg gccttatccg ggcgcagtcc ggcaagcaca ccccctcgga gcaggcggat
     2161 cgctggatcc aggtgctcgg cctgatcaag gtccacatca tctagcgagg cgcagcgggc
     2221 gccgaggcaa cgatagggag gaaccatgga cacgacactg agggcgcctg cgagggcgcg
     2281 gcacggcgcg cggatgagag ggctggtcct gctcggactg ctcgtctctt ccttcgctct
     2341 gctcctggcc ttgacgccga gggcggaggc cttcgtctac tggaccaacc agaccgggta
     2401 cagcatcggc cgcgccaaca acaacgccac cggcgtgaac cagaacttca tcaccggcct
     2461 cgatcgcgtc ttcgacgtgt acgtcgacga ctggcacatc tggtggacgg gctggaactc
     2521 catcggtcgc gcaagcctcg acggcaccaa cgtgaacaac agcttcatca ccggcatcac
     2581 ccccacaggc ctcaccgtct acgaccacca cgtctactgg tcagactact cgggctacat
     2641 cgggcgcgcg gcgacggacg ggaccggcat cgaccggacg tggatcaact gcaaggcgat
     2701 cccgtacaac gtgacgcagc acctcggcta cttctactgg aactggtcgg actcgtatgg
     2761 aaacgattac atcgcccgcg acaacccgac cgcggacggc gggaccgtga gccacatgga
     2821 cgtcctcggc gagtactgga tcgaattccc ctacggtctg tgcgccaccg cgagccacct
     2881 ctggtggcgg actgacgacg ccatcggccg cgcgagcacg ccgtacggtc tcagccccga
     2941 ccgcaactac atcactggtc tggagggcta cgggagcggt ctggccgagt acaacgacta
     3001 cctctactac acgacgtcag gcttctggga gggcccagca aacatcggcc gcgtgaagaa
     3061 cgacggcagc gggctcaaca acgcgcacat cacgggctgc tcgacgccgt ccggcgtctt
     3121 cgtcaacggc gggagtgccg cgacgagcgt cgtcgccctg gccggccaga tcgacgcggc
     3181 cgagccgacg gcgggccgcc tcccgtcatt ggtgaagaag ctcacgaacg ccaaggcggc
     3241 cctcgacagg ggcaacgtca agacggcgcg gaacatcgtg gatgcggcga tcaacgagat
     3301 cgaggcccag tcgggcaaga tgatcccggc ggagacggcc gagcgctgga tcaccgcgct
     3361 cacgctcatc agggtgcaca tcgtctagcg gggacgctca gatgcaagac ggggggcggc
     3421 ggaccggcgg tacgccgccc cccgtcgcgt gcttccgcat gatctccggc atgtggtact
     3481 cgacggccgt cgtgcgactg tgactgcctg gacaaaaggg tcctccgagc gcgcaagcgc
     3541 cccttcaggc tgcccgcgcg ggatcgatga gactgaagac ctgctgcagc gcctcacgtc
     3601 ggctcgcgac cagggggaac gcggtacgca ggcccgcgat catgagtgtc cggctaatga
     3661 gcgtaggatc gttcgttctt ggccgattcc ggaacctcgt cgtaaggcgg atctccgctc
     3721 cggcgtccct gaacactgtc ccccgggact gtctctgact gtccctcggg acattgtgcg
     3781 ccgcctgcgc ctgaccaaga gttgtgggca tggggacccg caaagaaaca agtatgggtg
     3841 agcggcgcat tctcctctac ggcgactcgg tcgtcttggg gagcgtaggc gcgagcctcg
     3901 gccggactcc tcagttcgag ctgatccatc tcgagacccc gcgggccagt gcagccgagc
     3961 tcgaggcatt ccggccagac gtcatcctct tcgacataga gaacgggcat ccggaggcgg
     4021 ccttctgcct gctcgagagc gatccagacc tgctgctgct cgggatcagc cctgacgtca
     4081 acctggtgcg gctctggacg ggccgccagt acagcgaact ctctgccgat gcgctggcgg
     4141 cgcttgccgc cggcgggaca gggcaggcac cgaaatcacc tcgaaaggac ggcgagtcat
     4201 gagcaggttg gtctcgctga ggtagtccgc gtcacgtcgg cgcagaagat cgagaactgg
     4261 cgccgcgggc gccgaggcaa aagggaggaa ccatggacac gacactgagg gcgcctg
//
LOCUS       DASUMX010000216         4014 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_76968, whole genome shotgun sequence.
ACCESSION   DASUMX010000216 DASUMX010000000
VERSION     DASUMX010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4014
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76968"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VFZ86_10360"
     CDS             <1..237
                     /locus_tag="VFZ86_10360"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF014316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEX5642720.1"
                     /translation="GPARLAQALGIGRAQNGLPVWEPPLAVLPRPADAPGPDVVTTAR
                     IGVRGGDQKPWRFVDAGSRFLSRPLSRTPILPSG"
     gene            274..1275
                     /locus_tag="VFZ86_10365"
     CDS             274..1275
                     /locus_tag="VFZ86_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NGG1p interacting factor NIF3"
                     /protein_id="HEX5642721.1"
                     /translation="MKLREIYETAVRMGIEADPRGKEGVDEFLARTKKRYDALPDHLK
                     ELADPEALTNPFNDTRIYVGDPEAEVKTLLGGIDMNTGEVLLADRLREKGVPVDAIYT
                     HHPESWGLTRLDSVMEVQADIWATLGVPIQAGEKLIFERMDEVRRRLMPINYDQAIDA
                     ARLLDMPFFSAHTPTDNLVVDFLNKWFAKKKPSTIEDVQKALLEIPEYRIAAQKGAGP
                     YAGKSGGTSRAGKIWVDMTGGTEGPKTVVEKLADAGVGTIVGMHMGQELRDEADKHSI
                     HVVIAGHMSSDSLGINLFLDELERGGVKTIATSGLIRVHRDKRGKVKSEEPGALPLR"
     gene            1345..3783
                     /locus_tag="VFZ86_10370"
     CDS             1345..3783
                     /locus_tag="VFZ86_10370"
                     /inference="COORDINATES: protein motif:HMM:NF013316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEX5642722.1"
                     /translation="MTVPELLAPAGEPRALRAALSAGADAVYFGLARWSARAFAGNFA
                     GEAAPEAVETAHLYDARAYLALNTLLKDDEVEPALAALEAPYLAGLDALIVADLGFAA
                     RVREEYPDLPLHASTQLNTHSSAQLEALRRLGFSRAILARELSLAEIAALETRGLELE
                     AFVHGALCYGYSGDCLLSSMVGGRSGNRGRCSQSCRLRYALRRTGSAALEPSRIMSTS
                     DLSAIGVLPELLAAGVSSFKVEGRMKDAAYVGVTTAVYREALDAAVADPERYDVHTTW
                     SARLEQSFSRSFTAAHLQGRHDEVRSGGRGGHRGVLVGRVSATDEGTGSVEIRLSQPV
                     AEGDVVYLYTPWGQTEPRRLEAGGDASLTLRVHERVALKDRLFRLEAADVGELGRDLV
                     TGRVALRPLELRMRLTGEVGSPATLAVTESRSGHAVPVETAGPLAAARTAALTEARAA
                     DALGALGGTPYRLSGLEFAVAGEVFLAVSELKDLRRRAVAALGERRLAAGRRTAGRAA
                     EGSDGGTGPDARSAAGTAGADAAQYADRAAAPPPVVLRLRVGESPLPLPQGGAYCLEV
                     LAGDAPAAVARSLEELRASGAHVRARLPEVLFDADAAWLAELLALPWHALVARAAGVL
                     PHLTGAFVTEYPLQGLNGLAAGVAAGLAGRWPPAVTASPEAALEEVAALAAALAAAAR
                     ARGQAPPAVEILAFGRQQVLHTRDLLGRAEGLYEAPGPGEHVSLLLEDAKGYEFPAEA
                     DAGGTRVFNARVTNLAPNLDELRAAGVATFVVEQAAMDQAERAAFAAGGLEALAPLAS
                     RERSTTGHLFRGVA"
BASE COUNT          525 a         1531 c         1467 g          491 t
ORIGIN      
        1 ggcccggcgc ggctcgccca ggcgctcggc atcggccggg cccagaacgg cctgccggtc
       61 tgggagccgc cgctggcggt cctgccgcgg ccggcggacg cgcccggtcc cgacgtggtc
      121 accacggcgc gcatcggcgt ccgcggcggc gaccagaagc cgtggcgctt cgtcgacgcc
      181 ggcagccgct tcctctcgcg cccgctctcg cgcacgccca tcctgccctc tgggtaagat
      241 aggagggatt ccagttcacc agaaaggacc accgtgaagc tccgcgagat ctacgagacc
      301 gccgtgcgca tgggcatcga ggccgacccg cgcggcaagg agggcgtcga cgagttcctg
      361 gcgcgcacca agaaacggta cgacgcgctc ccggaccacc tcaaagagct cgccgacccg
      421 gaagcgctca ccaacccctt caacgacacg cgcatctacg tcggcgatcc cgaggccgag
      481 gtcaagaccc tgctcggcgg catcgacatg aacaccggcg aggtgctcct tgccgaccgc
      541 ctgcgcgaga agggcgtccc tgtcgacgcc atctacaccc accacccgga gtcgtggggc
      601 ctcaccaggc tggacagcgt gatggaggtc caggccgaca tctgggcgac gctcggcgtg
      661 cccatccagg ccggcgagaa gctcatcttc gagcgcatgg acgaggtcag gcgccgcctc
      721 atgcccatca actacgacca ggccatcgac gccgcccgcc tgctcgacat gccgttcttc
      781 tcggcgcaca cgcccaccga caacctcgtg gtcgacttcc tgaacaagtg gttcgcgaag
      841 aagaagccca gcaccatcga ggacgtgcag aaggcgctgc tcgagatccc cgagtaccgc
      901 atcgcggcgc agaagggagc cgggccgtac gcgggcaaga gcgggggtac gtcgcgcgcc
      961 ggcaagatct gggtcgacat gaccggcggc accgagggcc ccaagacggt cgtggagaag
     1021 ctggcggacg ccggcgtcgg caccatcgtc ggcatgcaca tgggccagga gctgcgcgac
     1081 gaggccgaca agcacagcat ccacgtcgtc atcgccggcc acatgagcag cgactcgctg
     1141 ggcatcaacc tgttcctcga cgagctcgag cgcggcggcg tgaagacgat cgccacgagc
     1201 ggcctgatcc gcgtgcaccg cgacaagcgc ggcaaggtca agagcgaaga gcccggcgcg
     1261 ctgccgttgc gctagcgccg ggcccgggtc tcgcccggca ggaccgtgcc gccgaccgac
     1321 cagcccgcgt ccgcgggccg cagcgtgacc gtcccggagc tgctcgcgcc ggcgggggag
     1381 ccgcgcgccc tgcgcgcggc cctctccgcc ggcgcggacg ccgtctactt cggcctcgcg
     1441 cgctggagcg cgcgcgcctt cgccggcaac ttcgccggcg aggcggcgcc ggaggcagtc
     1501 gagaccgccc acctgtatga cgcgcgcgcg tacctggcgc tcaacacgct gctcaaggac
     1561 gacgaggtgg agccggcgct cgcggcgctg gaggcgccgt acctcgccgg cctcgacgcg
     1621 ctcatcgtcg ccgacctcgg cttcgccgcg cgcgtgcgcg aggagtatcc ggacctgccg
     1681 ctccacgcca gcacgcagct gaacacgcac agctcggcgc agctggaggc gctgcggcgg
     1741 ctcggcttct cgcgcgccat cctggcgcgc gagctcagtc tcgccgagat cgcagcgctc
     1801 gagacgcgcg ggctcgagct cgaggccttc gtccacgggg cgctgtgcta cggctactcc
     1861 ggcgactgcc tgctgagcag catggtcggc ggccgcagcg gcaaccgcgg ccgctgcagc
     1921 cagtcgtgcc gcctgcgcta cgcgctgcgg cgcaccggct cggccgcgct cgagccgtcg
     1981 cgcatcatgt ccaccagcga cctctcggcg atcggcgtgc tgccggagct gctcgcggcc
     2041 ggcgtctcga gtttcaaagt cgagggccgc atgaaggacg ccgcctacgt cggcgtgacg
     2101 accgccgtgt accgcgaggc cctcgatgcg gcggtcgccg accccgagcg ctacgacgtg
     2161 cacaccacgt ggagcgcgcg gctcgagcag agcttctcgc gctccttcac cgcggcgcac
     2221 ctccaggggc gccacgacga ggtccgcagc ggcggccgcg gcgggcatcg cggcgtcctc
     2281 gtcggtcgcg tgagcgcgac ggacgagggg acaggctccg tggagatcag actgagccag
     2341 cccgtggccg agggcgacgt cgtctatctg tacacgccgt ggggccagac ggagccgcgg
     2401 cggctggagg cgggcggcga cgcgagcctc acgctgcgcg ttcacgagcg cgtcgccctc
     2461 aaggaccgcc tcttccgcct ggaggccgcg gatgtcggcg agctgggacg cgacctcgtc
     2521 accggccggg tcgcgctgcg gccgctcgag ctgcgcatgc gcctgaccgg ggaggtgggg
     2581 tcgccggcca cgctcgccgt gaccgagtcg cgctcggggc acgcggtccc cgtggagacc
     2641 gcgggaccgc tcgcggccgc gcggacggcc gcgctcacgg aggcccgcgc ggcggacgcc
     2701 ctcggcgccc tcggcggcac gccctaccgg ctctcgggtc tcgagttcgc cgtggccggc
     2761 gaggtgttcc ttgcggtcag cgagctcaag gacctgcggc ggcgggccgt ggcggcgctg
     2821 ggcgagcgcc gcctggccgc cggtcgccgc accgccggcc gcgctgcgga gggctcggac
     2881 ggcggcacgg gcccggatgc gcggagcgca gccgggacgg cgggcgcgga cgccgcgcag
     2941 tacgccgacc gcgccgccgc accgccgccg gtcgtcctga ggctccgcgt cggcgagtcg
     3001 ccgctcccgc tgccgcaggg cggcgcctac tgcctggaag tgctggcggg cgacgctccc
     3061 gcggcggtcg cccgctccct cgaggagttg cgcgcatccg gcgcccacgt gcgcgcgcga
     3121 ctgcccgagg tcctcttcga cgccgacgcg gcctggctcg ccgagctgct cgcgctgccg
     3181 tggcatgcgc tcgtcgcgcg cgccgccggc gtcctgccgc atctgaccgg cgcgttcgtc
     3241 accgagtacc cgctgcaagg cctcaacggg ctcgccgccg gggtggccgc cggcctggcc
     3301 ggcaggtggc cgccggccgt cacggcatcg ccggaggcgg cgctcgagga ggtcgcggcc
     3361 ctggcggcgg cgctggcggc cgcggcgcgc gctcgaggcc aggcgcctcc ggccgtcgag
     3421 atcctcgcct tcgggcggca gcaggtgctg cacacccgcg accttctggg ccgggcggag
     3481 gggctctacg aggcgcccgg cccgggcgag cacgtctcgc tgctgctgga ggacgccaag
     3541 ggctacgagt tcccggcgga ggcggacgcg ggcggcacgc gcgtgttcaa cgcgcgcgtc
     3601 acgaatctgg cgccgaatct ggacgaactg cgcgcggccg gcgtcgccac cttcgtcgtc
     3661 gagcaggcgg cgatggacca ggccgagcgc gccgccttcg cggccggcgg cctggaggcg
     3721 ctcgcgccgc tggcgtcgcg cgagcgctcc accaccggcc acctcttccg cggcgtggcc
     3781 tgaccgttct ccggccgggc cccggctcgc cctgtgctat catccacggc ggccgcgccg
     3841 cccggcgcgg gctctcgtgc ctcgcgcccg gagcctccgg ccggcccgtc cgagtcgccg
     3901 tacccgttcg aaaggaccga gccgtggccg acagccgcct cctggacgac ctcatgatcg
     3961 gcgccgtcga cgtgctgccc gagggcgcgc tcgaggccaa gctcgacctc gccg
//
LOCUS       DASUMX010000217         2241 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_2090312, whole genome shotgun sequence.
ACCESSION   DASUMX010000217 DASUMX010000000
VERSION     DASUMX010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2090312"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1187)
                     /locus_tag="VFZ86_10375"
     CDS             complement(<1..1187)
                     /locus_tag="VFZ86_10375"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
                     /protein_id="HEX5642723.1"
                     /translation="MRARQVGITDTTLRDGHQSLWATRMHTSEMIPVLELMDSIGFRS
                     LECWGGATFDASLRFLDEDPWERLRLIKRHVVRTPLQMLLRGQNIVGYRHYGDDIVRR
                     FVYKAAENGMDVFRVFDALNDIRNYETAAAAIKETGKHFQGAVVYTISPVHSLDHYMD
                     HAHRLVDMGADSICIKDMAALLSPFYAEQLVGRLKAELSVPIQLHCHYIGGLAPMTYL
                     KAIEAGVDVIDTATVPLAFGNSQPATEMVVSALTGTPYDSGLDLEKLFEMAKYFEKVR
                     LEGGWDRGVTSLTHMQVYSHQVPGGMISNLESQLKEQSALDRLPEVLQEIPIVRAEVG
                     FPPLVTPLSQIVGTQAVLNVLSGRRWHIVPDEMKQYLRGKYGAAPGPISREVLLRVLE
                     GEEP"
     gene            complement(1209..1775)
                     /gene="efp"
                     /locus_tag="VFZ86_10380"
     CDS             complement(1209..1775)
                     /gene="efp"
                     /locus_tag="VFZ86_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010898947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEX5642724.1"
                     /translation="MADVVNTNQFKTGMAIEIDGQPFTIVEFQHVKPGKGGAFVRTKV
                     RNIATGAVLDKTFRAGEKFDRMHTQSRKMAFLYATDEEVVLMDNETYEQVSVSPAMAE
                     GALKWVVENMEVEIMYLNGVPFEVDAPNFVELTITKSDPGVQGNTAQGATKPATLESG
                     ATIQVPLFIEEGEKVKVDTRSGEYLGRA"
     gene            complement(1881..>2241)
                     /locus_tag="VFZ86_10385"
     CDS             complement(1881..>2241)
                     /locus_tag="VFZ86_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEX5642725.1"
                     /translation="LRHMYDAVLAAQLAGLAAVRAGAHGQADVDASARRVIAEAGYGE
                     RFGHGTGHGVGLEVHEAPALGRVRGDRLEAGMVCTVEPGVYVEGLAGVRIEDTVLVTP
                     DGCERLTTSPKELQVVD"
BASE COUNT          322 a          734 c          800 g          385 t
ORIGIN      
        1 ggctcctcgc cctccaggac gcgcagcagg acttcgcgcg agatgggtcc cggtgcggcg
       61 ccgtactttc cgcgcaggta ctgcttcatc tcgtcgggca cgatgtgcca gcgccgcccg
      121 gagagcacgt tgagcaccgc ctgggtgccg acgatctggc tcaggggcgt caccagcggc
      181 gggaagccga cctcggcgcg gacgatgggg atctcctgca ggacctcggg cagcctgtcc
      241 agcgcgctct gctccttgag ctggctctcg aggttgctga tcatgccgcc ggggacctgg
      301 tgggagtaca cctgcatgtg cgtgagcgag gtgacgccac ggtcccaacc accctccagg
      361 cggaccttct cgaagtactt ggccatctcg aagagcttct cgaggtccag cccgctgtca
      421 tacggcgtgc ccgtgagcgc ggacacgacc atctcggtcg ccggctgcga gttgccgaag
      481 gccagcggca cggtggccgt gtcgatgacg tccaccccgg cctcgatcgc cttgaggtag
      541 gtcatcgggg cgaggccgcc gatgtagtgg cagtgcagct ggatgggcac ggagagctct
      601 gccttgaggc ggccgacgag ctgctcggcg tagaacggcg agagcagcgc cgccatgtcc
      661 ttgatgcaga tggagtcggc gcccatgtcg acgagccggt gggcgtggtc catgtagtgg
      721 tcgagcgagt gcaccggcga gatggtgtac acgacggcgc cctggaagtg cttgccggtc
      781 tccttgatgg cggcggcggc cgtctcgtag ttgcggatgt cgttgagcgc gtcgaagacg
      841 cgaaagacat ccatcccgtt ctccgccgcc ttgtacacga agcggcgcac gatgtcgtcg
      901 ccgtagtgcc ggtagccgac gatgttctgg ccacgcagca gcatctgcag aggcgtgcgc
      961 acgacgtgcc tcttgatgag ccgcagacgc tcccaggggt cttcgtcgag gaaccgcagg
     1021 gacgcgtcga aggtggcgcc gccccagcac tcgagcgagc ggaagccgat cgagtccatg
     1081 agctcgagga ccgggatcat ctcgctggtg tgcatgcgcg tggcccacag cgactggtgg
     1141 ccgtcgcgca gcgtggtgtc ggtgatgcct acctgacggg ctcgcatgtg ctctcctgcc
     1201 ccgagcgctc aggcgcgccc gagatactcc ccgctgcggg tgtcgacctt gaccttctcg
     1261 ccctcctcga tgaagagcgg tacctggatg gtcgcgccgg attcgagggt ggcgggcttg
     1321 gtggcgccct gggcggtgtt gccctgcacg ccgggatcgg acttggtgat ggtgagctcg
     1381 acgaagttcg gcgcgtccac ctcgaacggc acgccgttga ggtacatgat ctcgacctcc
     1441 atgttctcga cgacccactt gagcgcgccc tccgccatgg cgggtgagac cgagacctgc
     1501 tcgtaggtct cgttgtccat gagcacgacc tcttcgtcgg tcgcgtacag gaacgccatc
     1561 ttgcgcgact gggtgtgcat gcggtcgaac ttctcgccgg cgcggaaggt cttgtcgagc
     1621 accgcgccgg tggcgatgtt gcggaccttg gtgcgcacga acgccccgcc cttgccgggc
     1681 ttgacgtgct ggaactcgac gatggtgaac ggctgaccgt cgatctcgat ggccatgccg
     1741 gtcttgaact ggttcgtgtt gacgacgtct gccatgggtc ctactcaggg gtcgaggggg
     1801 ctggtctgcg cacgtggtag gccatgatac cgcaaggctg cccgggtgtg caggcgcggg
     1861 gacggagacc gccgcgcgcc tcagtcgacg acctgcaact ccttgggcga ggtcgtgagg
     1921 cgctcgcagc cgtccggcgt cacgaggacg gtgtcctcga tgcggacgcc ggcgaggcct
     1981 tcgacgtaga cgcccggctc gacggtgcac accatgccgg cctcgagccg gtcgccgcgg
     2041 acgcgcccga gcgccggcgc ctcgtggacc tcgagcccga cgccgtgccc ggtgccgtga
     2101 ccgaagcgct cgccgtaccc ggcctcggcg atcacccgcc gggcggacgc gtccacgtcc
     2161 gcctggccgt gggcgcccgc gcgcaccgcg gcgaggccgg cgagctgcgc cgcgaggacg
     2221 gcgtcgtaca tgtggcgcag c
//
LOCUS       DASUMX010000218         4627 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_797592, whole genome shotgun sequence.
ACCESSION   DASUMX010000218 DASUMX010000000
VERSION     DASUMX010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4627
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_797592"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..1039)
                     /gene="argF"
                     /locus_tag="VFZ86_10390"
     CDS             complement(77..1039)
                     /gene="argF"
                     /locus_tag="VFZ86_10390"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014733865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEX5642726.1"
                     /translation="MPEKAKRHLSELDLTGRSFLTIHDYTPAEVVTLLDLAAALKDVQ
                     KRRVPHAFLEGRAVAMIFMKTSTRTRMSFEVGIEQLGAQPVFLNAGDIQLRVGETIKD
                     TANVMSRYVDCIMIRTFAQQDVEDLARYGQIPVINGLTDDHHPCQGMADALTIREHVG
                     RLEGVKLAYIGDGNNVAHSLAEVGAKTGMHVVICTPEDYRPDAAIIARAQADAAETGA
                     TVTLTANVEAAVANAHVIYTDTFTSMGQEAEHDARLAAMKAYQVNAELLELAGPQVKV
                     MHCLPAHWGEEITEEVLYSESSVVFDQAENRLHAQKAIMAALIA"
     gene            complement(1074..2396)
                     /locus_tag="VFZ86_10395"
     CDS             complement(1074..2396)
                     /locus_tag="VFZ86_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HEX5642727.1"
                     /translation="MAERTKVLIMGAAGRDFHNFNTYFREREQYEIVAFTATQIPYIN
                     DRKYPAELAGPYYPDGIPIHDESEMPELVKKHGVEQVVFAYSDVPYEYVMHRSAIVNA
                     LGADFRLMGPDKVMVKSTKPVIAVCAVRTGAGKSQTTRKIQTMLREAGKKVVSIRHPM
                     PYGDLVAQRVQRYATLDDLKKYECTIEEMEEYEPHVMAGNVIYAGVDYEAIVREAEKE
                     ADVILWDGGNNDFSFYAADLYITVADPHRPGDGLVYYPGEVNLRLADAVVLNKIDTAD
                     RAGIEAELEAVRSVNPDAQIIMGASPTTVDKPELIKGKRVLVVEDGPTLTHGEMKYGA
                     GTVAARQFGAAEFIDPRPFAVGEIKATFEKYPNVGVLLPAMGYSEQQLKDLETTINTS
                     GADVVVIGTPMDLRRIVDIKLPAVQVGYDLQEIGSPTLADVLREHKFI"
     gene            complement(2442..3512)
                     /locus_tag="VFZ86_10400"
     CDS             complement(2442..3512)
                     /locus_tag="VFZ86_10400"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/ornithine racemase family PLP-dependent
                     enzyme"
                     /protein_id="HEX5642728.1"
                     /translation="MPARATVTVDLGKVRANARTVVDHLPGISVVAVTKVTCGSPQVA
                     RAMLAGGARALGESRLENAQRLRDAGLTAPVWLLRAPPPELAGETVQLTDVSLQSELE
                     TVVALENAAAAAGRRHAIVAMVDLGDLREGMLPDALPAFLQRVNAMDHIDIAGIGVNL
                     TCYGAIVPDERNLGELATLARQSEQQLGRPLLVSGGNSGSIGMVTSGRMPASVDTLRI
                     GETILLGVDTLTREPTLGLHRDAFTVSAPVIECLVKPSLPRGTSAQDAFGTFPAFSDR
                     GERRRAICALGRQDAPPEGLTPVDPRVEVLGASSDHLILDVDALDRPPALGEPISFVP
                     TYAATLQLFTSPYVHKVFTPPA"
     gene            complement(3599..4384)
                     /locus_tag="VFZ86_10405"
     CDS             complement(3599..4384)
                     /locus_tag="VFZ86_10405"
                     /inference="COORDINATES: protein motif:HMM:NF018533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEX5642729.1"
                     /translation="MPEGDTIHHAARRIAAVLAGHVPDEITTPHPRFGRDRWPQRLAG
                     RRLEAADALGKHLLLRFEGGLVVHSHLRMTGAWRVLRRGERWPRSARSAWLVLRRGEV
                     EVVQFNGPVLELLTAARARGDPRLASLGPDLVASRPFDEARFLRRLRQDDPSRTLGDV
                     LLDQHVVAGIGNFWKSEACWRAGIDPWRRIADVADEEALAVVRGVRPLMQESAGHGRQ
                     DVFRTIYGRAGLPCPRCGAPALIQARGQGDDNRTTYWCPVCQR"
     gene            complement(4377..>4627)
                     /locus_tag="VFZ86_10410"
     CDS             complement(4377..>4627)
                     /locus_tag="VFZ86_10410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642730.1"
                     /translation="VSGRPVLSLHAGGRSLRRLGRPDDLEPALVALAAAVRAGRLPRI
                     VMDTIDGEPAVASPLAASLVGLGFRRGLHDFSLAPGDA"
BASE COUNT          636 a         1542 c         1708 g          741 t
ORIGIN      
        1 cgtcctcggg ttgccgatct cccccaagta cttcgagcac atcatgtggt tcccgagctg
       61 gatctgagcc cgcgcctcag gcgatgagcg ccgccatgat cgccttctgg gcgtgcagcc
      121 ggttctccgc ctggtcgaag accacggagc tctccgagta cagcacctct tcggtgatct
      181 cctcgcccca gtgcgccggc aggcagtgca tcaccttgac ctgcgggccc gccagctcca
      241 gcagctccgc attcacctga taggccttca tcgccgccag gcgggcgtcg tgctccgcct
      301 cctgacccat gctcgtgaag gtgtccgtgt agatgacgtg ggcgttggcg accgcggcct
      361 cgacgttggc ggtgagcgtc acggtggcgc cggtctcggc ggcgtccgcc tgcgcccggg
      421 cgatgatggc ggcgtcgggc cggtagtcct ccggcgtgca gatcacgacg tgcatgccgg
      481 tcttggcgcc gacctcggcg agcgagtggg cgacgttgtt gccgtcgccg atgtaggcga
      541 gcttgacgcc ctcgaggcgg ccgacgtgct cgcggatggt gagcgcgtcg gccatgccct
      601 ggcaggggtg gtggtcgtcg gtgaggccgt tgatcaccgg gatctggccg taccgggcga
      661 ggtcctccac atcctgctgg gcgaaggtgc ggatcatgat gcagtcgacg tagcggctca
      721 tcacgttggc ggtgtccttg atggtctcgc cgacgcgcag ctggatgtcg ccggcgttga
      781 ggaagaccgg ctgggcgccg agctgctcga tgccgacctc gaagctcatg cgcgtgcgcg
      841 tgctcgtctt catgaagatc atggcgacgg cgcggccctc gaggaacgcg tgcgggacgc
      901 ggcgcttctg aacgtccttg agcgcggcgg cgaggtcgag cagggtgacg acctcggccg
      961 gcgtgtagtc gtggatggtc aggaagctgc ggccggtgag gtcgagctcg gagaggtgac
     1021 gcttggcctt ctcgggcatg gtaccccctt cgttcagatg ccggagcagg ctatcagatg
     1081 aacttgtgct cgcggaggac gtcggcgagc gtcgggctgc cgatctcctg gaggtcgtaa
     1141 ccgacctgca cggccggcag cttgatgtcg acgatgcggc gcaggtccat cggcgtgccg
     1201 atgacgacca cgtcggcgcc ggaggtgttg atggtcgtct ccaggtcctt gagctgctgc
     1261 tcggagtagc ccatggccgg cagcagcacg ccgacgttcg ggtacttctc gaaggtcgcc
     1321 ttgatctcac cgacggcgaa cgggcgcggg tcgatgaact cggcggcgcc gaactgccgt
     1381 gcggccacgg tgccggcgcc gtacttcatc tcgccgtgcg tgagcgtggg gccgtcctcg
     1441 accaccagga cgcgcttgcc cttgatcagc tcgggcttgt cgacggtggt cggcgaggcg
     1501 cccatgatga tctgtgcgtc cgggttcacc gaacgcacgg cctcgagctc ggcctcgatg
     1561 ccggcgcggt cggcggtgtc gatcttgttc agcaccacgg cgtcggccag ccgcaggttg
     1621 acctcgccgg ggtagtacac gaggccgtcg ccgggacggt gcgggtcggc gacggtgatg
     1681 tacaggtcgg cggcgtagaa gctgaagtcg ttgttgccgc cgtcccagag gatgacgtcg
     1741 gcttccttct cggcctcgcg cacgatggcc tcgtagtcga cgccggcgta gatcacgttg
     1801 ccggccatga cgtgcggctc gtactcttcc atctcctcga tggtgcactc gtacttcttc
     1861 agatcgtcga gcgtcgcgta gcgctgcacg cgctgcgcga ccaggtcgcc gtacggcatg
     1921 gggtgacgga tggagacgac cttcttgccg gcctcgcgca gcatggtctg gatcttgcgc
     1981 gtggtctggc tcttgccggc tccggtgcgc acggcgcaga cggcgatcac cggcttggtg
     2041 ctcttgacca tgaccttgtc gggacccatg aggcgaaagt cggcgccgag ggcgttgacg
     2101 atggccgagc ggtgcatgac gtactcgtag ggcacgtcgg agtaggcgaa gacgacctgc
     2161 tcgacgccgt gcttcttgac cagctcgggc atctcggact cgtcgtggat cgggatgccg
     2221 tcggggtagt acgggcccgc gagctccgcc gggtacttgc ggtcgttgat gtacgggatc
     2281 tgcgtggccg tgaaggcgac gatctcgtac tgctcccttt cgcggaagta ggtgttgaag
     2341 ttgtggaagt cgcgccccgc ggctcccatg atcaggactt tcgttcgctc tgccatgctc
     2401 actcctgtgg ttgcaggtcc ctccgcgcca cgatccttgt gtcaggccgg cggagtgaac
     2461 actttgtgga cgtagggtga cgtgaacagc tgcagggtcg cagcgtaggt gggcacgaag
     2521 ctgatgggct ctccgagcgc gggcgggcgg tcgagcgcgt ccacgtccag gatgaggtgg
     2581 tcgctcgagg cgcccagcac ctcgacgcga gggtcgaccg gcgtcaggcc ctcgggcggc
     2641 gcgtcctggc gcccgagggc gcagatcgcg cgccgccgct ccccgcggtc gctgaaggcc
     2701 gggaaggtgc cgaacgcgtc ctgggcgctc gttccccgcg gcagcgacgg cttgaccagg
     2761 cactcgatga ccggcgccga gacggtgaag gcgtcgcgat gcaggccgag cgtgggctcg
     2821 cgcgtgagcg tgtcgacgcc gagcaggatg gtctcgccga tgcgcagcgt gtcgacggac
     2881 gcgggcatgc ggccggaggt gaccatgccg atggagcccg agttgccgcc cgagacgagg
     2941 agcgggcggc cgagctgctg ctcgctctgc cgcgcgagcg tcgccagctc gccgagattc
     3001 cgctcgtcgg ggacgatcgc cccgtagcac gtgagattga cgccgatgcc ggcgatgtcg
     3061 atgtggtcca tggcgttgac acgctggagg aaggcgggca gcgcgtccgg cagcatgccc
     3121 tcgcgcaggt cgccgaggtc gaccatggcg acgatcgcgt gccgccgccc cgcagccgcg
     3181 gcggcgttct cgagcgccac caccgtctcg agctcgctct gcagcgagac gtcggtgagc
     3241 tggacggtct cgccggccag ctccggcggc ggcgcgcgca gcagccagac cggcgccgtg
     3301 aggccggcgt cgcgcaggcg ctgggcgttc tccaggcgcg actcgcccaa cgccctcgca
     3361 cctcccgcga gcatggcccg agccacctgg ggcgagccgc aggtgacctt ggtgacggcg
     3421 acgaccgaga tgccgggaag gtgatcgacg acggtgcgcg cgttcgcgcg caccttgccc
     3481 agatcgaccg tgactgtggc tctcgccggc atcgccctcc catgcataag atatatgcac
     3541 gagagtatcg ttcccgaccc tccccgcgca agcgcgatcc accgtttggg agggctgctc
     3601 agcgctggca gaccgggcac cagtaggtgg tgcggttgtc gtcaccctgc ccgcgcgcct
     3661 ggatgagcgc cggggcgccg caacgtgggc acggaaggcc ggccctgccg tagatcgtcc
     3721 ggaagacgtc ctggcggccg tgcccggcgg actcctgcat caggggccgg acgccgcgta
     3781 ccaccgccag cgcctcttcg tccgcgacgt ccgcgatccg ccgccagggg tcgatgcccg
     3841 cgcgccagca ggcctccgac ttccagaagt tgccgatgcc ggcgacgacg tgctggtcga
     3901 ggagcacgtc gcccagcgtg cgcgagggat cgtcctggcg caggcggcgg aggaaccgcg
     3961 cctcgtcgaa cggccgggag gcgacgaggt ccgggccgag cgacgcgaga cggggatcgc
     4021 cgcgcgccct cgccgccgtg agcagctcga gcacgggccc attgaactgg acgacctcca
     4081 cctccccccg tcgcaggacc agccacgccg agcgcgcgga ccgcggccag cgttccccgc
     4141 gccgcagcac acgccacgcg cccgtcatgc gcaggtgcga gtggacgacg aggccgccct
     4201 cgaagcgcag gagcaggtgc ttgcccaagg cgtcggcggc ctcgagtcgc cggcccgcga
     4261 ggcgctgcgg ccagcggtcg cggccgaacc gggggtgcgg cgtcgtgatc tcgtcgggca
     4321 catggcccgc gagcacggcg gcgatgcgcc gggcggcgtg gtggatggtg tcgccctcag
     4381 gcatcgccgg gcgccagcga gaaatcgtgc agcccgcgac ggaagcccag cccgacgaga
     4441 ctggcggcga gcgggctggc gacggcgggc tcgccgtcga tggtgtccat cacgatccgg
     4501 ggcaggcgcc cggcgcgcac cgcggcggcg agcgccacga gcgccggctc gaggtcgtcc
     4561 gggcggccga gccggcgcag cgagcgcccg cccgcgtgca gcgagagcac cggccggccg
     4621 ctgaccg
//
LOCUS       DASUMX010000219        11536 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_79618, whole genome shotgun sequence.
ACCESSION   DASUMX010000219 DASUMX010000000
VERSION     DASUMX010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 11536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11536
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79618"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="VFZ86_10415"
     CDS             complement(<1..413)
                     /locus_tag="VFZ86_10415"
                     /inference="COORDINATES: protein motif:HMM:NF021485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2202 domain-containing protein"
                     /protein_id="HEX5642731.1"
                     /translation="MKRSFLVLVVVAVTLGAFGAMLVAAHPSLAKTPLSGTEAADLQY
                     VREEEKLARDVYQELDGLYGEQIPIFAKIAVSENTHTLAIKALLDRYGVTDPAADDEH
                     GVFVNHELQKLYDELVESGSASLREGLLVGVVIEEL"
     gene            547..1911
                     /locus_tag="VFZ86_10420"
     CDS             547..1911
                     /locus_tag="VFZ86_10420"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002716216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HEX5642732.1"
                     /translation="MGKSERWSPDSWRAWPSEQQPEWPDADALEAVVAQLRALPPLVF
                     AGESRQLRRALAGVAAGDAFLLHVGDCAESFRDLSAPRIRDNLKIMLQMATVLTYGAA
                     LPVVKLGRVAGQFAKPRSSPTERVGGEELPSFRGHIVNDDAPEAAARVPDPARLLQAY
                     HQSAARLNLLRAFTKGGFADLRQVHLWNLEFVARSSEGRRYEGIARGIDDALRFMTAC
                     GIDLEREHQLHEVDLFTSHEGLILPYEEALTRRDSLSGDWYDCSAHLLWVGERTRDPG
                     GAHIEFFSGVENPLACKLGTAAGGDQVVALCERLDPGRDPGRLTLIVRLGADRVEDLL
                     PPLIRAVREAGHPVIWACDPMHGNGLLTHEGVKTRRFDDIMRELRGFFRVCRAEGVWP
                     GGVHLEFTGDDVTECIGGVEPVSEGDLGVRYSTLCDPRLNAKQSLDVAFQVAELLAPG
                     RPRR"
     gene            2019..2558
                     /locus_tag="VFZ86_10425"
     CDS             2019..2558
                     /locus_tag="VFZ86_10425"
                     /inference="COORDINATES: protein motif:HMM:NF024637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642733.1"
                     /translation="MQCGLSIGDTATFCQVCGTPVEPAATAPAAEPAPVAGPAPVAGP
                     APEAEPAGVEVAPEDALSLRMAETLRLLQDASGCESTDAARAADLYREAICGCLEATE
                     DPLGSEDVRRDLLRGFDRLSSVLQREGLPAEALEVADSAASLGLLDSPFEMDQEQFGA
                     LRDRRESLRHILHGDSAQL"
     gene            complement(2604..3101)
                     /locus_tag="VFZ86_10430"
     CDS             complement(2604..3101)
                     /locus_tag="VFZ86_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642734.1"
                     /translation="MPRWFSIAAIFIVTAFVLHAFLVIVSLAAGVVSGQGLLTMAGPV
                     TFIVEFGLAAVITYLIIKPRASTPPVLSDEEIDKRDADLEADARRRVQAIEQHYRDEQ
                     RLERLKAEVVKWREARDLRAYATEALTDLGDDDVPTGEGGSLRAELQWALEYADSIDP
                     LRARD"
     gene            3318..3905
                     /locus_tag="VFZ86_10435"
     CDS             3318..3905
                     /locus_tag="VFZ86_10435"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEX5642735.1"
                     /translation="MTLRERLTASVTDIRARGQRLVQLNLELLTSELKAKGRKFGAAI
                     GLFAAAGLLALYAVAFALATVAVLLALVLPLWAAMLIVTAALFVLVAILVLVGRDQLR
                     KIGDPQPAAAIAEAKVTADLVKANARGTAAGVRARMRPRPAAPTDGGQAPVAAPAVAA
                     SPDAPPSNAPPSNAPPPDSPPRDTPPPSDPGARDS"
     gene            3902..4192
                     /locus_tag="VFZ86_10440"
     CDS             3902..4192
                     /locus_tag="VFZ86_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642736.1"
                     /translation="MSDTPSVDVNASGEGAAAGAAKAPARPVAEIRADIERERAGLDR
                     SFAALRGDLDEAVDAGKQRAARARRKVVVAVPAAATALVAALFLRSRRRGRG"
     gene            complement(4250..4546)
                     /locus_tag="VFZ86_10445"
     CDS             complement(4250..4546)
                     /locus_tag="VFZ86_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642737.1"
                     /translation="MSYLATFVIGLVLTGAWLWAVARWAGFVVPTADLLIIVGLCSGL
                     AVLPGAGWILAMLFLTLLVVKLTEADPWPDVVLVVTGSGVVWVVAGITRALLAS"
     gene            4627..5745
                     /locus_tag="VFZ86_10450"
     CDS             4627..5745
                     /locus_tag="VFZ86_10450"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEX5642738.1"
                     /translation="MDDQMPRSRAAVRLARSFGRTALSSGRRWWIGGAVVVVLGAAVL
                     GRDDLALMARSFAGADWHWAALALVLMLASLALRSLALQVIVNALDAGRARLRDAFSA
                     TSIGLLANSLIPIKVGTVLSPYVLFVLLRRRGAQAAFPTILGVTLTERMFAIATFLAL
                     SLAFLGTLDVPAWAVNVLLVCAGLITVPLVAGVVFARRRDWVARECARGGPRVRRMGR
                     WLPELVESQRIMRRPLAVLGVTGTQVLAWLLQLAAAVAMLWAFHLEAAGLRGAALVIV
                     LTNLIGLIPATPGNVGTFQVAAVAALATYGVAAGPALAYALGLQALQLLVGIVAGLAA
                     LAAQDLTLVDMAGRSRQAAAALRNAEPAMTPAEDPLVM"
     gene            5822..7348
                     /locus_tag="VFZ86_10455"
     CDS             5822..7348
                     /locus_tag="VFZ86_10455"
                     /inference="COORDINATES: protein motif:HMM:NF019886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HEX5642739.1"
                     /translation="MKRSTVSTRQPPAGGPGGASHPDALLRELRRGERDAFVRYFELF
                     REPVYGFTRRLLGDEAAAVAATTETFAVVLRRAVLDERAADLETLTYRCALDACVARA
                     DAGGVPARPASETAGRFEAALDALDLRRRAALLLHDVHGLSAAGIAAVLAIAEEAAGA
                     ELFRAREEFRAAFEASSPRPEGRGCRQAEQAVAGAVGLGLAGDELQRLRLHAAYCRPC
                     RRAMRAWGAAAVGLAAVLEPAPLPEALAAAPVFGTAIAAAPVPSPRAGLLAALRPAAH
                     FVRSRTVAYVVAVACLALAAGVAIHAQGVRPSILFQSVGPAVRLVTGSPGEAQPGSGQ
                     SEQESARPSPSVVTRAAFVVAGVEPQAAAPVVAPAPDTAAPAASADGAPDVAPREVAA
                     GDPETVAGGSAGAKAVTGRSGKDNAGGRSGKAESDRGRSVAAKAEHGRPESERAAASS
                     RRASASRATAAKATRKATAAKKAARADTRSREAQKPKKHAKPGKHAKPGKPAKPRKGG
                     "
     gene            7359..8393
                     /locus_tag="VFZ86_10460"
     CDS             7359..8393
                     /locus_tag="VFZ86_10460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX5642740.1"
                     /translation="MTDGPGPGVAGADAVARSRLLLQAAETINSSLDSGALETTILGE
                     AARLTGAGAAALLVPRGDVLVAREALGIGDRARDSFVVPLETSIYGRALVSGEIVVAE
                     LEEPEAGGAAEPAPLSALPDRPWRAVMAAPLQSHRAAYGVLALLFADAHPCSDEERAV
                     VRTLAIHAAIALDNRRLMREKEHMAVRDGLTDVFNRGYLELTLERTVKDVRRNGGDVS
                     ILFVDVDGMKDVNDTHGHHAGDVLLIELAHLLRDSCRETDVVARYGGDEFVVLMPATG
                     AAGAAAVERKVEAAIAARNAAATAPPYLSASTGTHTATAEGVDTLLQEADRRMYAVKR
                     ARGGRGGSPD"
     gene            8467..9057
                     /locus_tag="VFZ86_10465"
     CDS             8467..9057
                     /locus_tag="VFZ86_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642741.1"
                     /translation="MPRYIAIHPHACTERDLRRLTARHDEMPEDTLWNRSWCGVNKPV
                     AYCDWEAADEQAVARVLGAFRVPYSAIHRVTYFRGDAPDGLAARVRVCHDGNGSPRRF
                     IAVHPVSLEEEPLKRLVSRWREELPRDVLWRCTWAGVDAPISFCDWEAPCEEAVACVF
                     GKTGVPCDALHEVRYFDRRLSVWHDPEHPLLEPAVA"
     gene            9149..10090
                     /locus_tag="VFZ86_10470"
     CDS             9149..10090
                     /locus_tag="VFZ86_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020494654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX5642742.1"
                     /translation="MYAVVIEQGQVRWQERPDPEPGDHEILVAVRGAGVNGADISQRA
                     GTYPAPPGWPQDIPGLEMAGEVVVAGRRVTRFGAGDRVMALVGGGGQAELALVDEAHA
                     LPVPAGLSWEEAGGFMEACATAYDALFRQAGLTLGDRVLVTGAAGGVGSAGVQLAALA
                     GARVVASVRDAAQREAVAALGATEVVDPAEVAARGPYDVVLELVGAASLPSALGALAT
                     GARVVVIGIGGGSVVEIDLKALMRSRAGIRASTLRARPGGEKAVLVRQLGDRVVPALA
                     EGRLRVPVAAAFRLADAAAAYERFTAGGKLGKVVLVA"
     gene            10256..11113
                     /locus_tag="VFZ86_10475"
     CDS             10256..11113
                     /locus_tag="VFZ86_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012145904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HEX5642743.1"
                     /translation="MGDPTFVPNSTGKLRKVLLCPPAYFDFRPINEITRKVLADGEHA
                     DLDAVRREHEGFAQAYRDAGVEVVLMEPDPGLPYMVYARDFGACLAEGALIGSFREPV
                     RQGEDLLYERKLRELGVPIIGRVDRGFFEGGDFWFLDEATIAHGVVARSDWEGVRAAA
                     RILEPLGYAVVGVQLPSRNLHLDMAFNIVAPGVAVCAPEQLPDFFLRMLEKRRFELVE
                     VESAGVFKHHCNLQCLGDGRVLTFSGNTAVNQKLTALGLEVLTPELTQILKGGGGPHC
                     MTFPLLRDA"
     gene            11106..>11536
                     /locus_tag="VFZ86_10480"
     CDS             11106..>11536
                     /locus_tag="VFZ86_10480"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX5642744.1"
                     /translation="MPERAFIDLSRPLHPGMPVFPEDPEVAFAPAAALEPWRVTRLSL
                     GTHSGTHIDAASHYIPGGSTIDAYPLERFVLPSFVVPVAAGEDQAVEWPDLAATLPDS
                     LDGAAVLLHTGWDRHWGSPRALRHPFLSEAAAAGLVDRGAGL"
BASE COUNT         1487 a         4236 c         4236 g         1577 t
ORIGIN      
        1 agctcctcga tgacgacgcc gaccaacagg ccttcccgga gggaggcgct gcccgactcc
       61 acgagttcgt cgtagagctt ctgcaactcg tggttcacga acacgccatg ttcgtcgtcc
      121 gccgcggggt ccgtcacgcc atagcggtcc aggagagcct tgatagcgag ggtgtgcgtg
      181 ttctcggaca cggcgatctt cgcgaagatg gggatttgtt ctccgtagag gccgtcgagc
      241 tcttggtaga cgtcgcgggc gagcttctcc tcctcgcgca cgtactgcag gtcggcggcc
      301 tcggtcccgc tgagcggcgt cttcgcgagc gacggatgcg cggcgaccag catcgcgccg
      361 aaagcgccga gcgtgacggc gacgaccacg aggacgagga acgatcgttt catcggaccc
      421 tcctgagttg gacgactcgt gcacctctgt tgacgcagcg gggcggcgaa cgttacgcgc
      481 cgcgtcgttg tgcagcgtgt ccgcgatatc ctaggaggcg gacgccgact gaggaggacg
      541 tgcatcgtgg ggaagagcga gcgttggagc ccggactcct ggcgtgcctg gccgtcggag
      601 cagcagcccg agtggcccga cgccgacgcg ctcgaggcgg tcgtcgcgca gctcagggcg
      661 ctgcctcctc tggtgttcgc cggcgagtcc cggcagctgc gccgggccct tgccggggtc
      721 gccgcgggag acgccttcct cctccacgtc ggcgactgcg ccgagtcgtt ccgcgacctc
      781 tcggcgccgc gcatccgcga caacctcaag atcatgctgc agatggcgac ggtgctcacc
      841 tacggggcgg cgctgccggt cgtgaagctc ggccgcgtgg cggggcagtt cgccaagccg
      901 cgctccagcc cgaccgagcg cgtcggcggc gaggagctgc cgtcgttccg cgggcacatc
      961 gtcaacgacg acgcccccga ggcggccgcg cgtgtccccg atcccgcgcg gctgctgcag
     1021 gcctaccacc agtcggccgc gcggctcaac ctcctgcgcg ccttcaccaa gggcggcttc
     1081 gccgacctga ggcaggtcca cctctggaac ctcgagttcg tggcgcgctc gtccgagggg
     1141 cgccgctacg aagggatcgc gcgcggcatc gacgacgccc tgcggttcat gaccgcctgc
     1201 ggcatcgacc tggagcgcga gcaccagctc cacgaggtcg acctcttcac gagccacgag
     1261 ggcctgatcc tgccctacga ggaggcgctc acgcggcgcg acagcctgag cggcgactgg
     1321 tacgactgct cggcccatct gctgtgggtc ggcgagcgca cgcgcgatcc cggcggcgcg
     1381 cacatcgagt tcttctcggg ggtcgagaac ccgctggcgt gcaagctcgg gacggctgcc
     1441 ggcggcgacc aggtcgtggc gctctgcgag cggctcgacc ccgggcggga cccgggcagg
     1501 ctgacgctca tcgtccggct cggcgccgac cgggtggagg acctcctgcc gccgctcatc
     1561 cgcgccgtac gcgaggcggg gcatcccgtg atctgggcct gcgacccgat gcacggcaac
     1621 ggcctgctga cgcacgaggg ggtcaagacc cgccgcttcg acgacatcat gcgggaactg
     1681 cgcggcttct tccgcgtctg ccgcgccgag ggcgtctggc cgggcggcgt gcacctcgag
     1741 ttcacaggag acgacgtcac ggagtgcatc gggggcgtgg aaccggtgtc cgagggcgat
     1801 ctcggcgtgc gctactcgac tctctgtgac ccgaggctca acgcgaagca gtcactcgac
     1861 gtcgccttcc aggtggcgga gcttctggcg cccggacgtc cacgcaggta gcgtgcagct
     1921 gaccgcgcga ggtccccacc ctacggcttc tccggattct gccgatactg cggtgtggac
     1981 gaggcacgcg acgaaggaga gaggccgtgc gtgcgtgtgt gcagtgcgga ttgtcgatcg
     2041 gcgacaccgc gaccttctgc caggtgtgtg ggacgcccgt cgagccggcg gcgacggccc
     2101 ctgcggccga gccggctccc gtggccgggc cggctcccgt cgccgggccg gctcccgagg
     2161 ctgagcccgc cggcgtcgag gtcgcacccg aggacgccct gtctctccgc atggccgaga
     2221 ccttgcgact gctgcaggac gcctccggat gcgaatcgac ggacgcggcc cgggccgccg
     2281 acctgtatcg agaagcgatc tgcggctgcc tggaggctac cgaggatcct ctcggctcgg
     2341 aggacgtgcg ccgggacctg ctccgcggtt ttgaccgcct cagcagcgtg ctgcagcggg
     2401 agggacttcc ggcggaggcg ctcgaggtgg ccgacagcgc ggcgtcgctc ggcctgctcg
     2461 actctccgtt cgagatggac caggagcagt tcggcgcgct gagggatcgc cgagagagcc
     2521 tgcgccacat cctccacgga gactccgccc agctctgacc gggcggcggc gtcgcccggc
     2581 cggtcacact ccgccggcag gagtcagtcc cgggcgcgca gcgggtcgat gctgtccgcg
     2641 tactcgagcg cccactgcag ctcggcgcgc agcgatccac cctcgcccgt ggggacgtcg
     2701 tcgtcgccga ggtccgtcag cgcctcggtg gcataggccc gcaggtcgcg cgcctcacgc
     2761 cacttgacca cttcggcctt gagccgctcg agccgctgct cgtcgcggta gtgctgctcg
     2821 atcgcctgga cgcggcgccg ggcgtcggcc tcgaggtcgg cgtcgcgctt gtcgatctcc
     2881 tcgtcgctga gcaccggggg ggtcgacgcc ctgggcttga tgatgaggta cgtgatcacc
     2941 gcggccaggc cgaactcgac gatgaaggtg acgggacccg ccatggtcag gaggccctgc
     3001 ccggagacca cgcccgcggc cagcgagacg atcaccagga aggcgtgcag gacgaacgcg
     3061 gtgacgatga agatggctgc gatgctgaac catcgaggca cggtgtgatc ctccccccgc
     3121 gaacgtcggc ccgtccgcgc gtccgttccc tgaccgccac ccggcgcgac cggcattcgt
     3181 ggagtggctc gcaccatact agccgcaatc tccgcctggt gggaagcacc ttccgcctga
     3241 ggcggggagg tgtcgacgcc acgacggttt gtgccctgcg gacccgcggg tagggtcgca
     3301 tcgaccggag gtggagcatg acactgcggg aacgactgac tgcgtccgtc accgacatcc
     3361 gcgcgcgggg ccagcgcctc gtccagctca atctggaact gctgaccagc gagctcaagg
     3421 cgaagggccg caagttcggc gccgccatcg ggctgttcgc ggcagcgggc ctcctcgcgc
     3481 tctacgcggt cgcgttcgcc ctcgcgaccg tcgccgtcct gcttgccctc gtgctgccgc
     3541 tctgggccgc catgctcatc gtcaccgcgg cgctgttcgt gctggtcgcg atccttgtcc
     3601 tggtgggccg cgaccagctg cgcaagatcg gcgacccgca gcctgcggcc gccatcgccg
     3661 aggccaaggt cacggccgac ctcgtcaagg cgaacgcccg cgggaccgcc gcaggagtgc
     3721 gcgcgcggat gcgcccgcgg ccggccgcgc cgacggacgg cgggcaggcg ccggtggccg
     3781 cgccggcagt ggcggcgtca ccggacgcgc cgccgtcgaa cgcgccgccg tcgaacgcgc
     3841 cgccgccgga ctccccgccg cgggacaccc cgccgccatc cgacccggga gcgagggact
     3901 catgagcgac acgccgtcag tcgatgtgaa cgcctccggc gagggcgccg cggccggagc
     3961 cgccaaggcg ccggcgcgtc ccgtcgccga gatccgcgcc gacatcgagc gggagcgcgc
     4021 cgggctggac cgctcgttcg ccgccttgcg cggagacctg gacgaggcgg tcgacgccgg
     4081 caagcagcgc gccgcccggg cgcgccgcaa ggtggtggtg gccgtgccgg ccgccgccac
     4141 ggcgctggtc gcggccctct tcctgcgcag tcgcagacgc ggccgcggct aggcgaacgg
     4201 cgcttccggc ccctgaggcc ggtgagaccg ccgcagccgc cggttcccgt caggaggcga
     4261 gcagcgcgcg ggtgatgccg gccacgaccc acaccacccc cgagccggtc accacgagca
     4321 cgacgtcggg ccaggggtcg gcctcggtga gcttcacgac cagcagggtc aggaagagca
     4381 ttgccaggat ccagccggcg cccggcagga ccgccagccc gctgcacagt cccacgatga
     4441 tgagcaggtc ggcggtgggg acgacgaacc cggcccagcg ggccacagcc cacagccagg
     4501 cgcccgtcag gacgaggccg atgacgaagg tggcgaggta ggacatgcgg ccaccctacc
     4561 gcagcgagag tacgtcctga ttcgtgggtt gtgacccacg aatgctcggc gcatactcgc
     4621 gtgacgatgg atgaccagat gccgcgcagc cgggccgccg tgaggctcgc ccggtccttc
     4681 ggccggaccg cgttgtcctc cggccgccgc tggtggatcg gcggggcggt cgtcgtggtc
     4741 ctcggcgccg ccgtgctcgg gcgcgacgac ctggcgctga tggcacgctc cttcgccggg
     4801 gcggactggc actgggccgc cctggcgctc gtgctcatgc tggcctcgct ggccctgcgc
     4861 agcctcgcgc tccaggtcat cgtcaacgcc ctcgacgcgg gccgcgcccg gcttcgcgac
     4921 gcgttctcgg ccacgagcat cggcctgctg gccaactcgc tgatccccat caaggtgggt
     4981 accgtcctct cgccctacgt gctgttcgtg ctcctgcgcc gccgcggggc gcaggcggcg
     5041 ttcccgacga tcctcggggt caccctcacc gagcgcatgt tcgcgatcgc caccttcctc
     5101 gcgctgtcgc tggcgttcct gggcacgctg gacgtaccgg cttgggcggt caacgtgctg
     5161 ctggtgtgcg cggggctgat caccgtcccg ctcgtcgccg gcgtcgtctt cgcgcgccgg
     5221 cgcgactggg tcgcccgcga gtgcgcccgc ggcggcccgc gggtgcggcg catgggccgc
     5281 tggctgccgg agctggtcga gagccagcgc atcatgcgcc ggccgctggc ggtgctcggg
     5341 gtgacgggca cgcaggtgct cgcctggctc cttcagctgg cggcggcggt ggcgatgctc
     5401 tgggcgttcc atctcgaggc ggccgggctg cgcggagccg ccctggtcat cgtgctcacc
     5461 aacctcatcg gcctcatccc ggcgaccccg ggcaacgtcg gtaccttcca ggtggccgcg
     5521 gtcgcggcgc tcgccaccta cggcgtggct gcgggtccgg cgctcgccta cgctctcggc
     5581 ctgcaggctc tgcagctcct ggtggggatc gtggccgggc tggccgcgct cgccgcccag
     5641 gacctgacgc tcgtagacat ggccggcagg agccggcagg cggcggcggc gctccgcaac
     5701 gccgagcccg cgatgactcc ggcggaggat ccgctggtga tgtgagcctc ccgcaggcgt
     5761 ggtcggcagg ggcaacgccg gacgcttcag cgtcccgcga aggtgccgat agggagagca
     5821 gatgaagaga tccacggtat ccaccagaca gcccccggct ggtggccccg gcggcgcgtc
     5881 acacccggac gcgttgctgc gggagctccg ccgcggagaa cgtgacgcct tcgttcgcta
     5941 cttcgagctg ttccgcgagc ccgtctacgg attcacccgc cggctgctcg gcgacgaggc
     6001 ggcggcggtg gctgcgacca cggagacgtt cgcggtcgtc ctacgccgcg ccgtcctcga
     6061 cgaacgcgcc gccgatctgg agaccctgac ctaccgctgc gcgctcgacg cctgcgtcgc
     6121 gcgcgccgac gcgggcggcg tcccggcgcg gccggcgagc gagacggccg ggcgcttcga
     6181 ggcggcgctc gatgctctcg acctgcgccg ccgcgcggcg ctgctgctgc acgacgtgca
     6241 cggcctgagc gccgcgggta tcgccgccgt cctggctatc gccgaggaag ccgccggagc
     6301 ggagttgttc cgcgcccgcg aggaattccg cgcggcgttc gaagcgtcgt cgccgcgtcc
     6361 cgagggccgt ggctgcaggc aggccgagca ggctgtggcc ggcgccgtcg gtctcggcct
     6421 cgccggcgac gagctgcagc ggctgcgcct gcacgccgcc tactgccggc cgtgccggcg
     6481 ggcgatgcgc gcgtggggtg cggcggccgt cggtctggcc gcggtgctcg agccggcgcc
     6541 gctgccggag gcccttgcgg cggcgccggt cttcggcacc gccatcgcgg cggctcccgt
     6601 cccgtctccg cgcgccggcc tgctcgccgc gctgcgcccg gcggcgcact tcgtgcggag
     6661 caggaccgtc gcctacgtgg tcgcggtcgc ctgtctcgcc ctcgcggccg gggtcgccat
     6721 ccacgcgcaa ggcgtgcgtc cctccatcct cttccagagt gtcgggcccg cggtgcggct
     6781 ggtcaccggt tcgccgggcg aagcgcagcc cgggtcaggc cagagcgagc aggagtcggc
     6841 acggccgtcg ccgtccgtcg tcacgagagc ggccttcgtc gtggcgggcg ttgaaccaca
     6901 ggcggccgcg ccggtcgtgg cgccggcgcc cgacaccgcc gcacccgccg cttcggccga
     6961 cggcgctccg gacgtcgcgc cacgggaggt cgccgccggc gaccccgaga ccgtggccgg
     7021 cggctcggcg ggggccaagg ccgtgacggg gcgctccggc aaggacaacg ccggcgggcg
     7081 ttccgggaaa gccgagagcg accgcgggcg atccgtggcc gccaaggccg agcacggccg
     7141 ccccgagtcc gagcgcgccg ccgcgtcgtc gcgccgcgcc tcggcgtcgc gcgcgacggc
     7201 cgccaaggcg acgcgcaagg cgacggcggc caagaaggcc gcgagggccg acaccaggtc
     7261 aagggaagcc cagaagccca agaagcacgc gaagcccggg aagcacgcta agcccgggaa
     7321 gccggcgaag cccaggaagg gcggctgagg gccgcccgat gacggacggg cccggaccgg
     7381 gcgtcgccgg cgccgacgcg gtcgcgcgga gccgcctgct gctccaggcg gcggagacca
     7441 tcaactcgag cctcgactcc ggggcgctcg agacgacgat cctcggtgag gccgcgcgcc
     7501 tgaccggcgc cggcgcggcg gcgctgctcg tcccccgggg cgacgtgctg gtcgcgcggg
     7561 aggccctcgg gatcggcgac cgcgcccgtg acagcttcgt cgtcccgctc gagacctcca
     7621 tctacggacg tgcgctcgtc tccggcgaga tcgtcgtcgc cgagctcgag gagccggagg
     7681 ccggcggcgc ggcggagccg gcgccgctct cggcgctgcc cgacaggccg tggcgcgcgg
     7741 tgatggcggc tccactccag agccaccgtg ccgcgtacgg cgtgctggcg ctcctcttcg
     7801 cggatgcgca cccgtgcagc gacgaggagc gcgccgtggt gcgcacgctc gcgatccacg
     7861 ccgcgatcgc cctcgacaac cgccgcctca tgcgcgagaa ggagcacatg gccgtccgcg
     7921 acggcctgac cgacgtcttc aaccgcgggt acctcgagct cactctggag cgcacggtga
     7981 aggacgtgcg ccgcaacggc ggggacgtga gcatcctgtt cgtcgacgtc gacgggatga
     8041 aggacgtcaa cgacacgcac ggccatcacg ccggtgacgt cctgctgatc gagctggccc
     8101 acctcctgcg cgacagctgc cgggagacgg acgtcgtcgc gcgctatggc ggcgacgaat
     8161 tcgtggtcct catgccggcc accggcgccg ccggcgccgc cgcggtggag cgcaaggtgg
     8221 aggccgcgat cgcggcgcgc aacgcggccg ccacggcgcc tccgtacctc tccgccagca
     8281 cgggcacgca caccgcgacc gccgagggcg tggacacgct gctgcaggag gccgaccgcc
     8341 gcatgtacgc ggtcaagcgc gcccgcggcg ggcgcggcgg ttcacccgat tgaggtgacg
     8401 cgcggccggc cgcgctccgg ctagcctcgg tcgcgtcacg cacgcgccgc caccggagga
     8461 gtggccatgc ccaggtacat cgcgatccat ccgcatgcct gcacggagcg agacctcaga
     8521 cgactcaccg cgcggcatga cgagatgccg gaggacaccc tgtggaaccg cagctggtgc
     8581 ggcgtgaaca agccggtggc ctactgcgac tgggaagccg cggacgagca ggccgtcgcg
     8641 cgggtgctcg gcgcgttccg cgtcccgtac agcgccatcc accgcgtgac gtacttcagg
     8701 ggggacgcgc ccgacgggct cgcggcccgg gtgcgcgtct gccacgacgg caacggcagc
     8761 ccgcgccgct tcatcgccgt gcaccccgtg tcgctcgagg aggagccgct caagcggctc
     8821 gtcagcaggt ggcgcgagga gctcccccgt gacgtcctgt ggcgctgcac ctgggccggg
     8881 gtcgacgcac ccatctcctt ctgcgactgg gaggcgccct gcgaggaggc cgtcgcctgc
     8941 gtgttcggca agaccggcgt gccctgcgac gcgctccacg aagtgcgcta cttcgaccgc
     9001 cggctgagcg tctggcacga tccggagcat ccgctcctgg aaccggcggt cgcgtaagag
     9061 agcccgccga cggcggcgcc tcgggcgttg cgtgatggga gaaccggcgg cgcgggtaag
     9121 agccggcgga acgaacgagg aggccggcat gtacgccgtc gtgatcgagc agggacaggt
     9181 gcgctggcag gagcgtccgg acccggagcc cggggaccac gagatcctgg tggccgtgcg
     9241 gggcgccggg gtcaacgggg ccgacatcag ccagcgcgcg ggcacgtacc cggcgccgcc
     9301 cgggtggccg caggacatcc cgggcctcga gatggccggg gaggtggtcg tcgccgggcg
     9361 gcgcgtcacc cggttcggcg ccggcgaccg ggtcatggcg ctcgtcggcg gcggcggcca
     9421 ggccgagctg gcgctggtgg acgaggccca cgcgctgccg gtccccgcgg ggctgtcctg
     9481 ggaggaggca ggcgggttca tggaggcctg cgccaccgcg tacgacgcgc tcttccgaca
     9541 ggccggtctc acgctgggcg accgcgtgct cgtcaccggc gcggccggcg gggtcgggtc
     9601 cgccggcgtg cagctcgcgg cgctcgccgg cgcgcgcgtc gtcgcctccg tgcgcgacgc
     9661 cgcccagcgc gaggcggtcg cggcgctcgg ggcgacggag gtcgtcgatc cagccgaggt
     9721 cgcggcgcgc ggcccgtacg acgtcgtgct cgagctcgtc ggcgccgcca gccttccctc
     9781 cgcgctcggc gcgctggcga ccggcgcgcg cgtcgtcgtc atcggcatcg gcggcggctc
     9841 cgtcgtcgag atcgacctca aggcgctcat gcggagccgt gccggcatcc gggcctccac
     9901 cctgcgcgcc cggccgggcg gggagaaggc cgtcctggtc cggcagctcg gcgaccgggt
     9961 cgtgccggcg ctcgccgagg gacggttgcg cgtgcccgtg gccgccgcct tcaggctcgc
    10021 cgacgccgcg gccgcctacg agcgcttcac ggccggcggc aagctcggca aggtcgtgct
    10081 cgtggcctga gcttgtggcg ggccgatcac gccaccaggt gcatctgcac agtttaacgc
    10141 gtcgttacgg tgcagacgca cccttccttc ggaaagcggc tccgcttact ctcgcggcga
    10201 ccgtgcccgc ccgcgggcac ggtcgccggt cggcgcacga gaggagcggc acgcgatggg
    10261 agaccccacg ttcgtcccca actcgacagg gaagctccgc aaggtgctgc tgtgccctcc
    10321 cgcgtacttc gacttccggc ccatcaacga gatcacccgc aaggtcctgg ccgacggcga
    10381 gcacgccgac ctcgacgcgg tgcggcgcga gcacgagggc ttcgcccagg cctaccgcga
    10441 cgccggcgtc gaggtcgtgc tgatggagcc ggaccccggc ctgccgtaca tggtgtacgc
    10501 gcgcgacttc ggcgcgtgtc tcgccgaggg ggcgctcatc ggcagcttcc gcgagccggt
    10561 gcggcagggc gaggacctgc tctacgagcg caagctgcgc gagctgggcg taccgatcat
    10621 cggccgcgtc gaccgtggct tcttcgaggg cggcgacttc tggttcctcg acgaggcgac
    10681 gatcgcccac ggcgtcgtcg cccgcagcga ctgggagggc gtgcgcgccg ccgctcggat
    10741 cctcgaaccg ctgggttacg cagtcgtcgg ggtgcagctg ccctccagga acctgcacct
    10801 cgacatggcg ttcaacatcg tggctccggg cgtcgccgtc tgcgccccgg agcagttgcc
    10861 cgacttcttc ctgcgcatgc tcgagaagcg ccggttcgag ctggtcgagg tcgagagcgc
    10921 cggcgtcttc aagcaccact gcaacctgca gtgcctcggc gacggccgcg tgctcacgtt
    10981 ctccggcaac acggccgtca accagaagct cacggcgctc gggctcgagg tgctgacgcc
    11041 cgagctgacg cagatcctga agggcggggg cggtcctcac tgcatgacct tccccctctt
    11101 gcgcgatgcc tgagcgggcc ttcatcgacc tgagccggcc gctccacccg ggcatgcccg
    11161 tgttcccgga agacccagag gtggccttcg cgcccgccgc cgcgctcgag ccctggcggg
    11221 tgacacggct ctccctgggc acgcacagcg ggacccacat cgacgccgcc tcgcactaca
    11281 tccccggcgg ctcgaccatc gacgcctacc cgctcgagcg gttcgtcctc ccgtccttcg
    11341 tcgtgcccgt cgccgccggc gaggaccagg ccgtcgagtg gccggacctc gccgccacac
    11401 tgccggactc gctcgatgga gccgccgtcc tgctgcacac cggctgggac cgtcactggg
    11461 gctcgccgcg ggcgctgcgc cacccgttcc tcagcgaggc cgccgccgcc ggactcgtgg
    11521 accggggcgc cgggct
//
LOCUS       DASUMX010000220         8343 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_367080, whole genome shotgun sequence.
ACCESSION   DASUMX010000220 DASUMX010000000
VERSION     DASUMX010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 8343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8343
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367080"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..785)
                     /locus_tag="VFZ86_10485"
     CDS             complement(<1..785)
                     /locus_tag="VFZ86_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010511028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein T"
                     /protein_id="HEX5642745.1"
                     /translation="MSGEAGPRLLRTPFHAQHVAAGAKMVDFGGWDMPVQYPTGIIAE
                     HLATRGGAGLFDVSHMGRFVLRGPGAVPFLQHVLTNNAEALDLLQAQYTMLPTATGGA
                     VDDAYLYRFVEGEYLLVVNASNRRKDWGHFHEHLSHFPDVEMTDETGEIAMLALQGKT
                     SRDILSGLIESGALPEPLRNRLSVATLRLGDDHRVEARIARTGYTGEPVAFELFVPAH
                     DGPALWDTLVGKGAVPVGLGARDTLRLEAGLPLYGHELGADPQG"
     gene            complement(782..1987)
                     /locus_tag="VFZ86_10490"
     CDS             complement(782..1987)
                     /locus_tag="VFZ86_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003803482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5642746.1"
                     /translation="MSAPTSPVAERISRLGTETAFAVSAEAGAWKALGHTVYPFHLGD
                     MDLPTPANITEAAFRAIKDGKTGYCPNAGIPELREALAADVAASHGVPYGPENVAVQP
                     GGKPVIEKFLLTLMDPGDEVLYPSPGFPIYESQIDFFGGVARPYGFIPGERDFLLDFD
                     AIEAAISPRTKLLIFNNLHNPTGAESTGEEIEALAELVLKHDLYVLADEAYWDVRYSG
                     RSRSIAALPGMRERTVILHTFSKKFAMTGWRLGGAIGPKDLIARIATLNVNQESCTTH
                     FVQWAGVEALTGDQSGPEEIVRVLQERRDVAVDLLDQIPGVSCYRPEATFYLFPDVTG
                     LMERKGFQTYDELRRAALEQTGCSFCTRLHFGRALPGEERRYVRIAYSGISATLIEEG
                     LGRLKEWAQ"
     regulatory      complement(2073..2184)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            2323..3345
                     /gene="trpS"
                     /locus_tag="VFZ86_10495"
     CDS             2323..3345
                     /gene="trpS"
                     /locus_tag="VFZ86_10495"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEX5642747.1"
                     /translation="MSQQADRPARRPRVFSGIQPSGTLHLGNYLGAVKNWVAMQETHD
                     CVYCAVDLHAITVRQVRAELRRNTVDLVNLFLASGVDPERSIVFVQSHVAAHSELMWL
                     LNTVTYMGELRRMTQFKDKTAGAEGESIAVGLFDYPVLMAADILLYQADAVPVGEDQK
                     QHVELTRDLAERFNNAFGKAFVVPEPIIREGGARVMSLDDPAKKMSKSAGSENSYIAV
                     MDAPDAIRRKIRRAVTDSGAEVKGGPDKPALTNLLDIHAALSGDSVAVIEARYEGKGY
                     ADFKTDLAEIVVEALAPIQARIRELEADKSYTLEVLTTGAERAEAVAGRTLAKARERI
                     GLVPRP"
     gene            3426..3830
                     /locus_tag="VFZ86_10500"
     CDS             3426..3830
                     /locus_tag="VFZ86_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642748.1"
                     /translation="MISIETMKFRTADMIGEEFPLTFWRGKGRIVASDDTSLSLLVRG
                     VRTDLTWDRLAVTWNRLLSNHTLTVDELGGEADGVGICSIFAYMQADGLCVLDADGML
                     TLAETEGVPVHQFADMSRPTSWAPWRRKIHGD"
     gene            3865..4764
                     /locus_tag="VFZ86_10505"
     CDS             3865..4764
                     /locus_tag="VFZ86_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015539219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HEX5642749.1"
                     /translation="MITLAATHRVFAFGGAVEPALRVPAGTRVRLETADCFDDQVRVP
                     GDVLDAIDWDRVNPATGPVYVDGVGPGDVLSVLIESIAFGTQGVMAVAPGFGVHAADF
                     REQVHRIVPIEDGRALIDDGVAVPLRPMVGVIGVAPAGDPVATASPGAHGGNMDTRLI
                     GEGAVLYLPVAQEGALFAAGDLHAAMGDGEICGTGVEVSGSVTVRLDVRSDLHVDEPV
                     LETRDLVVTIASAETLDDAAENATRHMSDLLTGRLGVSLEVATMLMSAGGDLQVSQIV
                     DPLRTARFALPKSVIAGLGGDLI"
     gene            4842..5717
                     /locus_tag="VFZ86_10510"
     CDS             4842..5717
                     /locus_tag="VFZ86_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HEX5642750.1"
                     /translation="MNEGYGAQGMVGRLRRVVMRRPGETMAAAEPAAWHYTSAIDLTE
                     ARAAHDAFADALRAWGVEVLYHEEPLPEHSDSVFVFDPVLVTDEGTLVLSMGKERRRG
                     EEGPLAQTLEDCGVPVFGRLEGEARAEGGDTLWLDHDTLAVGRGFRTNAEGVRQLRGL
                     LEPAGVSVQDYDLPYFTGPEACLHLLSLVSPVDVDLAVAYLPLMPTALWTELTRRGIR
                     LLEVPEEEFARTQATNVLAVAPRKCIMLDENPVTRQLLQMADCEVVTFPGAPLSFKAE
                     GGPTCLTRPVLRDVE"
     gene            5726..7054
                     /locus_tag="VFZ86_10515"
     CDS             5726..7054
                     /locus_tag="VFZ86_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012615344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HEX5642751.1"
                     /translation="MAELSAVERAALEALDLDGLVALLRELIAIPSLDGHETPAQRRV
                     AEWLAAEGFETDVWPIDLDELAGHPDWSHEVEREEALGVVGWIGERAVGATADGAGRD
                     LLLNGHIDVAPIGDEAAWTAPPWEPVLRDGRVYGRGAVDMKGGLCCALFAAKAIRDAG
                     PRLRGRLSVASVAGEEDGGTGTLAAIVRGHITHGAVITEPTRLRVVSAQAGSLMFRLT
                     VHGRSAHGCVREEGASAVEAFVPLLAALRRLEAERCGVEDGRAAVAGDPRSPLFAGYR
                     LPWPIEVGTVRAGDWASSVPDTLVAEGRYGVAVGEDVAAARRAFEDAIARAAAAHPWL
                     AAHPPEVEWWGGRFDPAVTDPADPIVKAVTGAVADVTGSAPPVEGVTYGADMRLLVNV
                     GGIPTVLFGPGDVRVAHMPDEYVPVDDLRTAARSLVLTALRFCGVRETLQ"
     gene            complement(7083..>8343)
                     /locus_tag="VFZ86_10520"
     CDS             complement(7083..>8343)
                     /locus_tag="VFZ86_10520"
                     /inference="COORDINATES: protein motif:HMM:NF027964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HEX5642752.1"
                     /translation="SHGGRDTMKVLIVGAGMQGQVLTWNLGRNPAVTEIVVADYDEQR
                     AAFVAGQVGNGKALSTFINAADSDAVAEAGEGCKLIVNAVIPEFNKSIMTACLKCGAA
                     YIDMAQGQTRTQTIDEAYLDQMTLAPEFEKQGQLALLSTGMDPGVTNTFAANGYEDLD
                     TCFEIRIKDYALFDSPVPLQVWSQETYYTDCAQPPLLYEDGEFKRVEIFGRREKYEFP
                     EPFGLGTVICHDHEEVSTLPRLLPKALGEKGLRYVDFKMGGDEEGIDADLALVGSGMT
                     SKYPVKLKDGSEVRPIDVFVATLAPNPPADELARLALAGEITDNGVVTVDCIGEKNGK
                     PASIGYFIFPPDIQWVNGQIPGATCVSYGTSTPASIYAEFIVEDKITQKGMVCPEELE
                     RGVRDAFIAEMGVRNMPAFRKETVAAN"
BASE COUNT         1213 a         2827 c         2988 g         1315 t
ORIGIN      
        1 ccttgtgggt ccgcgcccag ctcgtggccg tagagaggca ggccggcctc aagccgcagg
       61 gtgtcgcggg cgccgagccc gacgggcacg gcgcccttcc cgaccagtgt gtcccagagg
      121 gccggcccgt cgtgcgccgg cacgaagagc tcgaaggcga ccggttcgcc ggtgtagccg
      181 gtgcgggcga tgcgcgcttc cacgcggtgg tcgtcaccca gacgcagcgt cgccacgctc
      241 agcctgttgc gcagcggctc cggcagcgcc ccactctcga tcaggccact caggatgtcc
      301 cgggacgtct tgccctgcag ggccagcatc gcgatctcgc cggtctcgtc ggtcatctcg
      361 acgtcgggga aatgcgagag atgctcgtgg aagtgccccc agtccttgcg tcggttcgag
      421 gcgttgacga cgagcaggta ctcaccctcc acgaagcggt agagataggc gtcgtcgacg
      481 gcgcccccag tcgccgtcgg cagcatggtg tactgcgcct ggaggagatc cagggcctcg
      541 gcgttgttcg tgagcacatg ctgcaggaac ggcacggctc ccggaccacg gagcacgaag
      601 cgccccatat gcgagacgtc gaacaggccg gctccgccac gggtggcgag atgctcggcg
      661 atgatcccgg tcgggtactg gaccggcatg tcccagccac cgaagtccac catcttcgcg
      721 cccgcggcaa cgtgctgggc gtggaacggc gtgcgcagca ggcgcgggcc cgcctcgccg
      781 ctcactgcgc ccactccttg agccggccga ggccttcttc tatgagtgtg gcgctgatgc
      841 ctgagtatgc gatgcgcacg tagcggcgct cctctccggg cagcgcgcgg ccgaagtgca
      901 gacgggtgca gaagctgcag cccgtctgct ccagcgccgc gcgccgcagc tcgtcgtagg
      961 tctggaagcc cttgcgttcc atgagcccgg tgacgtcggg gaacaggtag aacgtcgcct
     1021 ccgggcggta gcagctgact cccgggatct ggtccagcag gtcgacggcg acgtcgcggc
     1081 gctcctggag gacgcgcacg atctcttcag gtcccgactg gtcaccggtg agcgcctcga
     1141 cgccggccca ttggacgaag tgggtggtgc acgactcctg attgacgttc agggtggcga
     1201 tgcgcgcgat gaggtccttc ggaccgatcg cgcccccgag ccgccagccc gtcatcgcga
     1261 acttcttgct gaacgtgtgc aggatgacgg tgcgctcacg catgccggga agcgccgcga
     1321 tcgaccggct gcgcccggag tagcgcacgt cccagtaggc ctcgtccgcc aggacgtaca
     1381 ggtcgtgttt caggacgagc tccgccagcg cctcgatctc ctcacccgtg ctctccgccc
     1441 cggtcgggtt gtggaggttg ttgaagatca gcagcttcgt gcgcggggag atcgcggcct
     1501 cgatcgcgtc gaagtccagc aggaagtcgc gttcgcccgg gatgaagccg tagggcctgg
     1561 cgacgccgcc gaagaagtcg atctggctct cgtagatggg aaagccgggg ctggggtaga
     1621 ggacctcgtc gccggggtcc atcagcgtga gcaggaactt ctcgatgacc ggcttgcctc
     1681 ctggttgcac ggcgacgttc tcgggtccgt aaggcacccc gtgcgaggcg gcgacgtcgg
     1741 cggccagcgc ctcgcgcagc tccgggatgc cggcgttggg gcagtatccg gtcttgccat
     1801 ccttgatggc gcgaaaggcg gcctcggtga tgttcgcggg cgtgggcagg tccatgtcgc
     1861 cgagatggaa ggggtagacg gtatggccga gcgccttcca cgcgccggcc tcggcggaga
     1921 cggcgaacgc cgtctcggtc ccgaggcggc tgatgcgctc ggccactggg ctggttggtg
     1981 cgctcatgcc gctcacccct taaatgtcga gggacagggt gatgtcccca tcaccctgtc
     2041 cgccgcggac gaggtccgcc actccagggg cccgtcccgt cgcgttccgt ttgcctgaga
     2101 gtttcggcgg cgcatccggc gccatccttg ccccttcggc gcccgcacgt tccttcgagt
     2161 tgcgggctct cacgcgacca tccaccgggc atcctcggtt ctcaggcact gtagcaccgc
     2221 gattccgcag gtcgcaacgt cggagcggca catctccgct cgacgggcct cgcgcgcgcg
     2281 gtcggtggtc tgccccgccc cgcttggata gactccggag ccgtgagcca acaggcagac
     2341 agaccagccc gccgcccgcg tgtcttcagc ggcatccagc cgtcggggac gctgcacctg
     2401 ggcaactatc tcggcgcggt caagaactgg gtcgcgatgc aggagaccca tgactgcgtg
     2461 tactgcgcgg tggacctgca cgcgatcacc gttcgccagg tccgcgccga gcttcgtcgc
     2521 aacaccgtcg acctggtgaa cctgttcctg gcgagcggcg tggacccgga gcgttccatc
     2581 gtcttcgtgc agagccatgt ggccgcgcac agcgagctca tgtggctcct caacaccgtc
     2641 acgtacatgg gcgagttgcg gcgcatgaca cagttcaagg acaagaccgc gggcgccgag
     2701 ggcgagagca tcgccgtcgg gctgttcgac tacccggtgc tcatggccgc ggacatcctg
     2761 ctgtaccagg ccgatgccgt gcccgtcggg gaggaccaga agcagcacgt ggagctcacg
     2821 cgcgacctcg ccgaacgctt caacaatgcc ttcggcaagg cgttcgtggt gcccgagccg
     2881 atcatccgcg agggcggcgc ccgcgtcatg agcctcgacg atccggccaa gaagatgagc
     2941 aagagcgccg ggtctgagaa cagctacatc gcagtgatgg atgcgccgga cgccatccgc
     3001 aggaagatcc ggcgcgcggt caccgactcg ggcgccgagg tcaagggcgg cccggacaag
     3061 ccggcgctga ccaacctgct cgacatccac gcagctctca gcggcgactc cgtcgccgtc
     3121 atcgaggcgc ggtacgaggg caagggctac gcggacttca agaccgacct ggccgagatc
     3181 gtcgtcgagg cgctcgctcc gatccaggcc cgcatccgtg agctcgaggc ggacaagagc
     3241 tacacgctcg aggtcctcac gaccggcgcc gagcgcgccg aagcggtcgc gggtcgcacg
     3301 ctcgcgaagg cccgcgagcg catcggactg gtgccgcggc cgtaaggacc ctgttgcggc
     3361 ggccgctcgt cacgcgactc gatgcccgct agactaggcg tctgcagcgg tcgcccggag
     3421 gcatgatgat ctcgatcgaa acgatgaagt tccgcaccgc cgacatgatc ggcgaggagt
     3481 tcccgctgac gttctggcgc ggcaagggcc gcatcgtggc ctccgacgac acgagcctgt
     3541 cgctgctcgt ccgtggtgtg cgcaccgacc tcacgtggga ccggctcgcg gtcacgtgga
     3601 accggctcct ctccaaccac acgctcacgg tggacgaact gggcggcgag gccgacggcg
     3661 tcggcatctg cagcatcttc gcctacatgc aggccgacgg gctgtgcgtg ctcgacgccg
     3721 acggcatgct cacgctggcc gagacagagg gcgtgccggt gcaccagttc gccgatatgt
     3781 cgcggcccac ctcgtgggct ccctggcgcc gcaagatcca cggcgactga cccccggacc
     3841 cgtcgcggcg acctccaagc ggccgtgatc acgctcgccg ccacccatcg cgtcttcgcc
     3901 ttcggcggtg ccgtcgagcc ggcgctgcgc gtgcccgcag gcacgcgtgt ccggcttgag
     3961 acggccgact gcttcgacga tcaggtgcgg gtgccgggcg acgtcctgga cgcgatcgac
     4021 tgggacaggg tgaacccggc cacgggcccc gtgtacgtgg atggggtcgg ccccggggac
     4081 gtcctcagcg tcctcatcga gtccatcgct ttcggtacac agggcgtgat ggcagtggcg
     4141 ccggggttcg gtgtccacgc agcggacttc cgcgagcagg tgcaccgcat cgtgccgatc
     4201 gaggacggac gtgcgctgat cgacgacgga gtggcggtcc cgctgcggcc catggtcggc
     4261 gtgatcggcg tcgcgccggc cggcgacccg gtcgcgaccg cgtctccggg cgcgcacggc
     4321 ggcaacatgg acacccggct gatcggagaa ggcgccgtgc tctacttgcc ggtcgcccag
     4381 gagggcgcgc tgttcgcggc aggcgacctg catgcggcga tgggcgacgg ggagatctgc
     4441 ggcaccggcg tcgaagtgag cggcagcgtc accgtgcggc tcgacgtgcg cagcgacctg
     4501 cacgtcgacg agccggtgct cgagacgcgc gatctcgtgg tgaccatcgc ctcggcggag
     4561 acgctcgacg acgccgcgga gaacgccacg cggcacatga gcgacctgct caccggccgc
     4621 ctcggcgtct cgctggaggt cgcgaccatg ctcatgagcg ccggcggcga cctgcaggtc
     4681 tcgcagatcg tcgatccgtt gcgcactgcc cggttcgcgc tccccaagag cgtcatcgcc
     4741 gggctcggcg gcgatctgat ctagcggatg cggctgccac tgcagagtcc ttacggctgc
     4801 gacccgacga cgcgacgttc ggccgggatc aagggggaat gatgaacgag ggctatggag
     4861 cccagggcat ggtgggacgg ctgcgccgcg tcgtgatgcg gcggcctggc gagacgatgg
     4921 ccgccgccga acccgcggcg tggcactaca cgagcgcgat cgacctcacg gaggcccgcg
     4981 ccgcgcacga tgcgttcgcc gacgcgctgc gcgcctgggg cgtcgaggtg ctgtaccacg
     5041 aggagccgct gccggagcac agcgacagcg tgttcgtctt cgacccggtc ctggtcaccg
     5101 acgagggcac gctcgtgctc agcatgggca aggagcgccg gcggggcgaa gaaggaccct
     5161 tggcgcagac gctggaggac tgcggcgtgc cggtcttcgg ccgtctcgag ggcgaggctc
     5221 gcgccgaagg cggggacacc ctgtggctgg accacgacac actggcggtc ggccgcgggt
     5281 tccggacgaa cgccgaaggc gtgcgtcagc tccgcggcct cctcgagccg gccggcgtct
     5341 cggtccagga ctacgacctg ccgtacttca cgggcccgga ggcctgcctc cacctgctca
     5401 gcctcgtcag cccggtcgac gtcgacctgg ccgtggccta cctgccgctc atgccgacgg
     5461 cgctgtggac cgagctcacg cgtcgcggca tccggctgct cgaggtcccg gaggaggagt
     5521 tcgcgcggac acaggcgacc aacgtgctcg ccgtcgcgcc gcgcaagtgc atcatgctgg
     5581 acgagaaccc ggtgaccagg cagctcctgc agatggccga ctgcgaggtc gtcaccttcc
     5641 ccggcgcgcc gctctcgttc aaggctgagg gcgggccgac ctgcctcacg cggccggtct
     5701 tgcgcgacgt ggagtgaggc aggcggtggc cgagctcagc gcggtcgaac gtgcagcgct
     5761 ggaggcgctc gacctcgacg gcctcgtggc gcttctgcgc gagctgatcg cgatcccgag
     5821 cctggacggg cacgagaccc cggcccagcg gcgcgtggcc gagtggctgg cggccgaggg
     5881 cttcgagacc gacgtctggc cgatcgatct ggacgagctc gccggtcatc cggactggag
     5941 ccacgaggtc gagcgtgaag aggcactcgg tgtggtcggc tggatcggcg agcgagcggt
     6001 cggggcgacg gcagacggag ccggccgcga cctgttgctc aacggccaca tcgacgtggc
     6061 tcccatcggc gatgaggcag cctggacggc gccgccctgg gagccggtcc tgcgggacgg
     6121 cagagtctac ggccgcggcg ccgtcgacat gaagggcggc ctgtgctgtg ccctgttcgc
     6181 cgccaaggcg atccgcgacg ccggcccgcg actgcggggc cgcctgagcg tcgccagcgt
     6241 ggcgggcgag gaggacggcg ggacaggcac cctggccgcc atcgtccgcg gccacatcac
     6301 ccacggtgcc gtgatcacgg aaccgacgcg cctgcgagtg gtctcggcgc aggcgggctc
     6361 gctgatgttc cgcctgaccg tacacggccg gtccgcacac ggttgcgtgc gcgaggaggg
     6421 cgcaagcgcg gtcgaggcgt tcgtgccgct gctggcggcg ctccggcgac tggaggcgga
     6481 gcgctgcggc gtggaggatg gccgtgccgc cgtcgccggc gatccccgct cgccgctctt
     6541 cgccggctac cgcctgccgt ggccgatcga ggtcggcacc gtacgcgccg gcgactgggc
     6601 gagcagcgta cccgacactc tcgtcgccga gggtcgctac ggcgtcgccg tcggcgagga
     6661 tgtggccgcg gcccggcggg cgttcgaaga cgcgatcgcc cgcgccgccg cggcccaccc
     6721 ctggctcgcc gcccatccgc cggaggtcga gtggtggggc gggcgtttcg acccggcggt
     6781 gaccgacccg gccgatccga tcgtcaaggc cgtgaccggc gccgtcgcgg acgtcaccgg
     6841 atcggcgcct ccggtcgagg gcgtcaccta cggcgccgac atgcgccttc tcgtcaacgt
     6901 cgggggcatc ccgaccgtgc tcttcggccc gggcgacgtc cgcgtcgccc acatgcccga
     6961 cgagtacgtg ccggtcgacg acctgcggac ggctgcccgg tcgctggttc tcacggcgct
     7021 gcgcttctgc ggcgtgcgcg agaccttgca gtgagcgtga cgccggagga cgttgtgctc
     7081 cttcagttgg cggcgacggt ctccttgcgg aaggccggca tgttgcgcac gcccatctcg
     7141 gcgatgaacg cgtcgcgcac gccacgctcc agctcctcgg ggcagaccat gcccttctgg
     7201 gtgatcttgt cctcgacgat gaactcggcg tagatgctgg ccggggtgct ggtgccgtag
     7261 ctgacgcagg tggctccggg gatctggccg ttcacccact ggatgtccgg cgggaagatg
     7321 aagtagccga tgctggccgg cttgccgttc ttctcgccga tgcagtcgac ggtgacgacg
     7381 ccgttgtcgg tgatctcgcc ggcgagggcg agcctggcca gctcgtcggc cggcgggttg
     7441 ggagccaggg tggcgacgaa gacgtcgatg ggccgtacct cgctgccgtc cttcagcttg
     7501 accgggtact tgctggtcat tcccgagccg acgagggcga ggtcggcgtc gatgccctcc
     7561 tcgtcgccgc ccatcttgaa gtcgacgtag cgcaggccct tctcgccgag ggctttgggg
     7621 agaagtcgtg gcagcgtgct gacctcttcg tggtcgtggc agatgacggt gccgaggccg
     7681 aacggctccg gaaactcgta cttctcgcgg cggccgaaga tctccacgcg cttgaactcg
     7741 ccgtcctcgt acagcagcgg cggctgagcg cagtcggtgt agtacgtctc ctgcgaccag
     7801 acctggagcg gcaccggtga gtcgaagagc gcgtagtcct tgatgcggat ctcgaagcac
     7861 gtgtcgaggt cctcgtaccc gttggcggcg aaggtattgg tgacgcccgg atccataccg
     7921 gtgctgagaa gggcgagctg accctgcttc tcgaactcgg gagcgagggt catctggtcc
     7981 agatacgcct cgtcgatggt ctgcgtgcgt gtctggcctt gagccatgtc gatgtaggcg
     8041 gcgccgcact tgaggcacgc cgtcatgatc gacttgttga actcggggat gacggcattg
     8101 acgatgagct tgcagccttc gccggcctca gcgacggcgt cgctgtcggc ggcgttgatg
     8161 aacgtggaca acgctttgcc gttgccgacc tgcccggcga cgaaggcggc gcgctgctcg
     8221 tcgtagtcgg cgacgacgat ctcggtgacg gccgggttgc ggccgaggtt ccaggtgagg
     8281 acctgaccct gcatgccggc gccgacgata aggaccttca tcgtgtctct ccctccgtgt
     8341 gac
//
LOCUS       DASUMX010000221         2793 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_942448, whole genome shotgun sequence.
ACCESSION   DASUMX010000221 DASUMX010000000
VERSION     DASUMX010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_942448"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VFZ86_10525"
     CDS             <1..193
                     /locus_tag="VFZ86_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002629955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2891 family protein"
                     /protein_id="HEX5642753.1"
                     /translation="RAWMLEGIASGLPAKDRRRAALLASSRAHAAAALPAVTGEHYEG
                     GHWLGTFAVYLTTRAGLRE"
     gene            complement(165..539)
                     /locus_tag="VFZ86_10530"
     CDS             complement(165..539)
                     /locus_tag="VFZ86_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642754.1"
                     /translation="MKTRNRDTNTTPGLPPPPAPTSRDANPWSMAEAQAPVRKGQPPA
                     AATPSRPTRFPTKPPSSVEPRRRPVVPLVILAFMAATAIGLVADALRSGSFEEAIGPV
                     VTVVFVAFMIWRAVTRAGRRGS"
     gene            728..2287
                     /locus_tag="VFZ86_10535"
     CDS             728..2287
                     /locus_tag="VFZ86_10535"
                     /inference="COORDINATES: protein motif:HMM:NF017591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase PhoX"
                     /protein_id="HEX5642755.1"
                     /translation="MTPSKLLSLTVALTLGAAVASADSFTGPQSSQTPYVVPTADGWE
                     VTSLITVGDSAKESPYVMVGIPDGMGAVAGKFAENGRYVADKAFMTVFLNHELGTTSG
                     VERAHGTKGAFVSQWTVHLNSLQVKWGEDLIRRVLTWQGGTFADTTGLAVFNRFCSGD
                     LPPLTGFFNSTTGNGFDGLIYMNGEEAGAEGRAFAHVVTGDEKGTSYELPYLGKFSWE
                     NSVAHPNSGDRTLVVGLDDSSPGQVYLYVGDKQDNGNPVERAGLQNGRLYGVQVTDGG
                     ANYAGGTVPRENAGAINGAFTLHAFTRDEAAGTGAALQALSVANGVTEFARPEDGHWD
                     TRNPNVFYWVTTGGSGQTARLYRFTFDSIQNPTGGTVELVVDAASLVGTDGATARSFD
                     NLVVDADGNILIQEDPGGTSYIAKTWKIDPTKSLGQDGFAVQIFESDRERFLLGGSSY
                     LTQDEENSGVIEVTDIVRNANWYEAGRRYYLGNTQAHYGIPGELVQGGQVYLMASPMA
                     AMPAVQPRKGK"
     gene            complement(2350..>2793)
                     /locus_tag="VFZ86_10540"
     CDS             complement(2350..>2793)
                     /locus_tag="VFZ86_10540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642756.1"
                     /translation="RLEPRATARAVETVNGAVTLAEDVLVSGDAGTVNGRLTLMRGAR
                     VDGRLENVNGALRLEGATVRGGIETVNGDVFVGTGSRVDGGIRVEKPGGGSWRKSDRL
                     PRITIESGAEVNGTLRFEREVELYVGAGATVGPVEGVAPKRQPLP"
BASE COUNT          445 a         1001 c          924 g          423 t
ORIGIN      
        1 ccgcgcgtgg atgctcgagg gcatcgcgag tggcctgccc gcgaaagacc gccgccgcgc
       61 ggcgctgctc gcctcgtcgc gtgcgcacgc cgccgccgcg ctgcccgcgg tcaccggcga
      121 gcactacgag ggcgggcact ggctcggcac gtttgcggtc tatctcacga cccgcgccgg
      181 cctgcgcgag tgaccgcccg ccagatcatg aacgccacga acacgaccgt gacgaccggg
      241 ccgatcgctt cctcgaaact gcccgacctc agggcgtcgg cgacgagccc gatcgcggtc
      301 gctgccatga aggcgaggat cacgagcggc acgacgggcc gcctgcgcgg ctcgacgctg
      361 ctgggcggct tggtcggaaa gcgcgtcggc cgacttggcg tcgccgcagc gggcggctga
      421 cccttcctga ccggcgcctg cgcctccgcc atcgaccagg ggttggcatc gcgcgaggtc
      481 ggcgccgggg gcggcggcag gcccggcgtg gtgttggtgt cccggttcct ggtcttcatc
      541 ctgcggacct cgcggcctgt ggctggcgcg tagcgacaga ttacgccggc gcgcgccgac
      601 caaaccaccc gcaaccgtca tcgaacgtaa acgcaggggt aaagaactgt tcatgcatcg
      661 cgcccacgat cctctcggcg tgcgggctgg cccaggcccg cgttcacccc aacccgtgga
      721 gagatgcatg accccgtcga aattgctttc cctgaccgtg gccctgaccc tgggcgccgc
      781 cgtcgccagc gccgacagct ttaccggccc acagagctcg cagaccccct acgtcgtacc
      841 gaccgccgat ggctgggagg tgacttcgct gatcaccgtg ggcgattcgg ccaaggagtc
      901 gccctacgtg atggtcggca tcccggacgg catgggtgcc gtggccggaa agttcgccga
      961 gaacggcagg tacgtcgccg acaaggcgtt catgactgtc ttcctgaacc acgagctcgg
     1021 caccacctcc ggcgtggagc gcgcgcacgg tacgaagggt gccttcgtgt cacagtggac
     1081 cgtgcatctc aactccctgc aggtgaagtg gggcgaagac ctgattcggc gcgtcctgac
     1141 ctggcagggc ggcaccttcg ccgacacgac tggcctggct gtcttcaacc gcttctgctc
     1201 gggtgacctg ccgcccctca ccggcttttt caactcgacg accggcaacg gattcgacgg
     1261 cctgatctac atgaacggcg aggaggccgg cgccgaaggg cgggcctttg cgcacgtcgt
     1321 caccggcgac gagaagggca cctcgtacga actgccctac ctcggcaagt tttcgtggga
     1381 gaactccgtc gcgcacccga actcgggtga caggacgctc gtggtcggac tcgacgattc
     1441 ctcgcccggc caggtctacc tgtacgtcgg agacaagcag gacaacggca atcccgtcga
     1501 gcgtgccgga ctgcagaacg ggaggctcta cggagtccag gtcacggacg gcggcgcgaa
     1561 ctacgccggc ggcaccgtgc cgcgcgagaa tgcaggcgcg atcaacggcg ctttcactct
     1621 gcacgccttc acgcgcgacg aggccgccgg cactggggcc gccctgcagg ccctgtcggt
     1681 ggccaacggc gtcacggagt tcgctcgtcc cgaagacgga cactgggata cacggaaccc
     1741 caacgtcttc tactgggtga ccaccggcgg cagcggccag acggcacggc tctaccgctt
     1801 caccttcgat tcgatccaga accccaccgg cggcacggtc gagttggtcg tcgatgcggc
     1861 gagcctcgtc ggcaccgacg gggccacggc acgctccttc gacaacttgg tggtggacgc
     1921 cgacggcaac atcctcatcc aggaggatcc gggcggcacg agctacatcg cgaagacctg
     1981 gaagatcgac ccgacgaagt cgctggggca ggacggcttc gccgtgcaga tcttcgaatc
     2041 ggaccgcgag cgcttcctgc tcggcggctc gagttacctg acgcaggacg aggagaactc
     2101 tggcgtcatc gaggtcacgg acatcgtgcg gaacgcgaac tggtacgaag ccggtcgccg
     2161 ctactacctc ggcaacacgc aggcgcacta cggcatcccg ggggagctcg tgcagggcgg
     2221 ccaggtttac ctgatggcgt cgccgatggc cgccatgcct gcggtgcagc cgcgcaaggg
     2281 caagtaatcc cttcgatccc cgcaacggac gcgggacggg ggcggtcgtt tcgaccgccc
     2341 ctttttcatt tacggcagcg gctggcgctt cggcgcgacg ccctcgacgg gccccacggt
     2401 ggcgccggcg cccacgtaca gctcgacctc gcgttcgaag cgcagcgtcc cgttgacctc
     2461 tgcgcccgat tcgatggtga tccgaggaag ccggtcggac ttgcgccacg acccgccgcc
     2521 cggcttctcg acccggatcc cgccgtccac ccgtgagccg gtgcccacga acacgtcgcc
     2581 gttcacggtc tcgatgccgc cgcgcaccgt cgcgccctcg agccggagcg cgccgttcac
     2641 gttctcgagg cgaccgtcca cgcgcgcacc ccgcatcagc gtcagcctgc cgttgaccgt
     2701 gccggcatcg ccgctcacca gcacgtcctc cgcgagcgtg accgcgccgt tcaccgtctc
     2761 gacggcacgc gccgtggcac gcggctcgag gcg
//
LOCUS       DASUMX010000222         2224 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_799989, whole genome shotgun sequence.
ACCESSION   DASUMX010000222 DASUMX010000000
VERSION     DASUMX010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_799989"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            176..805
                     /locus_tag="VFZ86_10545"
     CDS             176..805
                     /locus_tag="VFZ86_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005410764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642757.1"
                     /translation="MRKCTAATSAVLLCLALAPAFAVVPTQEAVTPAAAFMVALAEHC
                     GKAYAGRIVANEPATTPGSPPDPFEGKTLVMHVRECTPGEIRVPFHVGEDRSRTWVIT
                     RTDDGLRLKHDHRHEDGSPDVLTLYGGDTTAPGTATRQEFPADAESKALFERQGLKAS
                     VANVWSLEIVPAQRYVYELARPGRRFRVEFDLTTPVAPPPPPWGEAAAP"
     gene            910..1494
                     /locus_tag="VFZ86_10550"
     CDS             910..1494
                     /locus_tag="VFZ86_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HEX5642758.1"
                     /translation="MKIFSRLFDRALHWSRHPHAERYLAVVSFTESSFFPVPPDVLLA
                     PMTLARPQRWWRLAALTTVTSVLGGLLGYAIGYVALESVTPLLHRVGYWGHFETAHDW
                     FERYGFWAIFAAGFTPIPYKVFTIAAGAAHMALLPFTLGSLVGRGARYFLVAGLVRWG
                     GAPIEHHIRRYVDGIGWATLGILVVGYLAWTVLR"
     gene            1610..>2224
                     /locus_tag="VFZ86_10555"
     CDS             1610..>2224
                     /locus_tag="VFZ86_10555"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEX5642759.1"
                     /translation="MRIDRYVHDERLTWGAAGVLMAVTALHLSGIARLLVAPPETVAF
                     EAPPVFQVSLQKLPPPRLPPALQPRPADAPPPTDAVENRPRQTRPVPILVDTPTTDPV
                     PPDTQVKKYEIGDELLPGVPGPPMPEGTGDDEPLYADAAPVEETPVLWNLKLVDMRPP
                     VYPPRCLRLGIEGRVKIRVLVGENGRPQEVTVGESSGEASLDQAA"
BASE COUNT          329 a          807 c          743 g          345 t
ORIGIN      
        1 gatagaagtt cggcagcacc gcggccgtgg gcgacatcag catcgacagc ggcaggttga
       61 ccagcccgaa ggcgatcagc agcgaggtgg gcagtcggga cggcttcggc ggcatcgggt
      121 aggctgggtg cgggcccgga gatccgattg tcgttcatgt gccggaggat tgcccatgcg
      181 caagtgcacc gccgcgacgt ccgccgtact cctctgcctg gcgcttgcac ctgcctttgc
      241 ggtggtgccg acgcaggagg ccgtcacccc cgccgccgct ttcatggtcg ccctcgcgga
      301 acactgcggc aaggcctatg ccgggcgcat cgtcgccaac gagcccgcga ccacccccgg
      361 gtcaccgccc gatcccttcg agggcaagac gctggtgatg cacgtgcgcg aatgcacgcc
      421 gggcgagatc cgcgtcccct tccacgtcgg cgaggatcgc tcgcgcacgt gggtcatcac
      481 ccgcaccgac gacggcttgc gcctgaagca cgatcaccgt cacgaggacg gctcaccgga
      541 cgtgctgacc ctgtacggag gcgacaccac ggccccgggc acggcaacgc ggcaggagtt
      601 cccggccgac gccgagtcga aggcgttgtt cgagcggcag ggactcaagg cgtcggtcgc
      661 caacgtctgg tcgctcgaga tcgtcccggc gcaacgctac gtttacgagc tcgcgcgtcc
      721 gggtcggcgg ttccgcgtcg agttcgacct cacgacgccg gtcgcgccgc cccctccgcc
      781 gtggggcgaa gcagccgcgc cctgagtcct cgtcggccac gagaggcagg accgaccatt
      841 tcgcagattt gagccaggcc cagtggacca acggggttcg gcgcgccgtc tcccgctaaa
      901 atcctgcgga tgaagatctt ctcccggctg ttcgaccgcg ccctgcactg gagccggcac
      961 cctcacgcag aacgctatct cgccgtcgtc agcttcacgg agtcgtcgtt ctttccggta
     1021 cccccggacg tcctgctcgc gccgatgacg ctcgcgcggc cgcaacgctg gtggcgcctg
     1081 gccgcgttga ccaccgtcac gtcggtgctc ggcggactgc tcgggtacgc gatcggctac
     1141 gtcgcactcg agtccgtgac gccgctgttg catcgggtgg gctactgggg ccacttcgag
     1201 acggcgcatg actggttcga gcgctacgga ttctgggcga tcttcgccgc gggcttcacg
     1261 ccgatcccct acaaggtgtt cacgatcgcg gcgggggcgg cccacatggc cctcctgccc
     1321 ttcaccctcg gctcgctcgt cggccgtggg gcccgctact tcctggtggc gggactggtg
     1381 cgctggggag gcgccccgat cgagcaccac atccgccgct acgtggacgg catcggctgg
     1441 gccaccctcg gcatcctagt ggttgggtac ctggcttgga ccgtgctgcg gtaagtctct
     1501 ggtccgctag gtatttctgg ctgcgaccag ccgcagccca ggcgccgcaa atccggtaga
     1561 ttccaaggcg atcgcttcca tcccggaggg acgcgagtcc cggttcccga tgcgcatcga
     1621 ccgctacgtc cacgacgaac gcctgacctg gggcgcggcg ggcgtcctga tggccgtgac
     1681 tgcgctgcac ctcagcggga ttgcacgact cctggtcgcg ccgcccgaga ccgtggcgtt
     1741 cgaagcccca ccggtgttcc aggtcagcct gcagaagctg ccgccgccgc gactcccgcc
     1801 cgcgctccag ccgcggccgg cggacgcacc gccccccacc gacgccgtcg aaaaccggcc
     1861 acgccagacg cggcccgtgc cgattctcgt cgacacgccg accactgacc cggtcccgcc
     1921 cgacacccag gtcaagaaat acgagatcgg cgacgagctg ctgccgggcg tgcccggtcc
     1981 gcccatgccg gaaggcacgg gcgacgacga gccgctctat gccgacgcgg cgccggtgga
     2041 ggaaaccccg gttctctgga atctcaagct cgtggacatg cgcccccccg tctacccgcc
     2101 gcgctgcctg cgcctcggga tcgaagggcg ggtgaagatc cgcgtgctcg taggcgagaa
     2161 cggccggccc caggaagtca ccgtgggcga gtcgagtggc gaggcgtcgc tcgaccaggc
     2221 cgcc
//
LOCUS       DASUMX010000223         8101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1661750, whole genome shotgun sequence.
ACCESSION   DASUMX010000223 DASUMX010000000
VERSION     DASUMX010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 8101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8101
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1661750"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2617
                     /locus_tag="VFZ86_10560"
     CDS             <1..2617
                     /locus_tag="VFZ86_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642760.1"
                     /translation="LVLELARSVEASPDLITWRQGRALAYGDAAGFSALQEVLKAHAG
                     IMDSDDAASVERKLDRVLPEVEHRAWLRQRLGALLGLEVSSITEDESLIAWSQLLEHI
                     TASGPAILVLEDLHWAGEGMLAFLQRLAERRMAVPLLVLMTARPELAGRTLPLAAPIF
                     AVPLAPLTQMETAGLMSSLLQHPLPPDVLTPVLDKIGGNPLYAEEFTRLLQDRGLLAL
                     EDGQLRLRHGEELPVPASLRAVLAARLDGLPALHKELLGDAAVIGDTFWRGGVAEISA
                     RSAEIVESVMGSLAEHELVRSLATPSIAGEAEYTFWHALARDVAYSQLPRKARATKHE
                     AAAAWIERRAGPHASDFADMLAHHYCSALDLLGSIGETESADRLVEPAIRYLVLAGDR
                     AMNLDVAVAEQRYAKALELAGSTGPERFRVLAKWAGALAMEDRDLEAVRAYRDAVEGL
                     IGSGDVGAAALALCRLSRCLSSLGDPTAPDLPAQAVRLIEDMEPSSEQVFIYAVYAQV
                     LFIYGASHPETVIEAATRALEVAGALGLPAPAEALFWRGYARLDLGDLAGLEECERAV
                     RLASDQGLGQERARIEQMNAILALRVKGPRESVVACDRGLEFSRRRGLEAFVVEFRMS
                     RIEALCLAGEWDYALAEIRDLVPVLEEAEDTWHLLGVRISEALVLARRGLPSGAAGFI
                     EWLERQGRDHRAAQMRAGALLAAAAVRSAEGRDEVTLHLVAEFLRVTEEASAYLGVLE
                     DMPGVMRSAAACGGAQHAISLARRTAALVPVQRLPLDHAAAMTVAALADELGGDVRTA
                     AANHAEAAVAWHDLSVPYEEAQALLGQGRCLMTLDRPDEALEQFNAARPIFERLGAEP
                     ALAETDEQLGRAARS"
     gene            2949..3080
                     /locus_tag="VFZ86_10565"
     CDS             2949..3080
                     /locus_tag="VFZ86_10565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642761.1"
                     /translation="MPSVSLHQNAPEFSLLDFSGRPFSLLEYRGEKHVLLVFNRGFT"
     gene            3093..3434
                     /locus_tag="VFZ86_10570"
                     /pseudo
     CDS             3093..3434
                     /locus_tag="VFZ86_10570"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
     gene            3684..4112
                     /locus_tag="VFZ86_10575"
     CDS             3684..4112
                     /locus_tag="VFZ86_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642762.1"
                     /translation="MRRLWIVVAIVCGMLALASMASASMRGEVRSFVAAVAAVMPDGD
                     VTASPSPEPSLSATESAMPSPAPSAASAEEDVDETGAQGSHGTAVSTVARDKDAVGSK
                     TLANGKTITNHGMAVSAVARAKAGGGDGHSGGQGKGKAGH"
     gene            4470..5465
                     /locus_tag="VFZ86_10580"
     CDS             4470..5465
                     /locus_tag="VFZ86_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642763.1"
                     /translation="MSRVHPFQVFVWAVLVLSALLVVPATAAALDGSPPRVYLQADPA
                     VADNGPFGYGVWSTKSVFLEVVASDPESGVSKIEHAIAVPDSEEFVWLEGSKFSVADE
                     GVWWAVARATNGDGVTGVFLADDLAPKLWTGEWVSIDRTVPTTVASGAVSGHWYGTPV
                     TVTLAASDGAGGSGFLITEYRLDASGWTAGSDVPVSAQGKHTLRYRSSDLANNMEAGK
                     TLTFGIDTRRPKTKAPYAAGAARGAIATLKYKVADPRPGSPTAEVTIKVMNRSGKLVK
                     TLGPYAGRAVNTLLSARFTVPRSWSAGAYRFAVSAKDKAGNTQVLPAGSNRLVVR"
     gene            complement(5687..6568)
                     /locus_tag="VFZ86_10585"
     CDS             complement(5687..6568)
                     /locus_tag="VFZ86_10585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEX5642764.1"
                     /translation="MRRRPVLAVVLCLGMIALLGVGASPAAAATCVDCHAVPPDGPPA
                     PHGVLVAAVTDCITCHRGMSPHPASPRIPGLSLSGKSSAAGLVLAGKLWQPGALNLID
                     GPNGSYMLKVGLPDVTVYLQQRLWADTEFTDLTQVTTDAGMFSEGAYSYTVPSPVPFA
                     AYRAISQGAVLTPDFGSSSIWRPAAAVLLPKPRLTLKLSGLTNGSIALGRKLKVSGTV
                     APIDLAGQDVFVKWQRRVGRTWADVRDHFEKIGATGAYSWTFTVRQRGLFRIRAMIEA
                     TPDTAAAVTTWRRFTVK"
     gene            6760..7137
                     /locus_tag="VFZ86_10590"
     CDS             6760..7137
                     /locus_tag="VFZ86_10590"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5642765.1"
                     /translation="MGYTVFRPEERAFEPPQRGDQRRGRASLSEALHEMRANIWRLPA
                     GARGTRHLERVQEEMFVVLEGAATLLLGDPPERVELPRGSVAVVETGTALQLRNESDA
                     EVVVLIVGAPPVQGEAEYLPDVD"
     gene            7203..>8101
                     /locus_tag="VFZ86_10595"
     CDS             7203..>8101
                     /locus_tag="VFZ86_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEX5642766.1"
                     /translation="MGHATSLGGAHQDLAKRLQRGPVAMVEPRDPAARQAWREILETL
                     FSPEDAALAARLPVVPTTVADLARRTGLGAGALRARLDAMAGKGLVLDLPDARTGATT
                     YLLAPPVVGFFELSMMRLHDGLPKARLARAYEAYMAGDSAFDAEVSGIPTLISRTLVQ
                     ETALYDDLASEVLDWERATSYVERADCLAVTNCFCRHTAAHLGTACDYPMETCMSLGT
                     AARYLVRQGHARQVGREEGRELLEAAREHGLVHIGDNVRRELNYICSCCSCCCVELRS
                     AQKGMPVMQPSAFQPAVAGERCTG"
BASE COUNT         1222 a         2725 c         2833 g         1321 t
ORIGIN      
        1 cctggtgctc gaactcgccc gcagcgttga agcttcaccc gatttgatca cgtggcgcca
       61 ggggagagcg ctggcctacg gagacgccgc cggcttctct gccctgcagg aggtgctcaa
      121 ggcccacgca ggcatcatgg acagcgatga cgccgcttcc gtcgagcgca agctggaccg
      181 cgtgcttccg gaagtggagc atcgagcgtg gctccgccag cggctcggtg cgctcctcgg
      241 gctggaggtc tcttcgatca ccgaagatga gagcctgatc gcgtggtcgc agctgctcga
      301 acacatcacg gcctcgggtc cggcgatcct cgtgctggag gacctgcatt gggcgggtga
      361 gggcatgctg gcgttcctgc agcggctggc cgagcgccgc atggcagtcc cgctcctcgt
      421 gctcatgacc gctcggcccg aactggcagg gcgcacccta cctctggccg cgccgatctt
      481 cgcggtaccg ctggctcccc tcacgcagat ggagaccgct ggactcatgt cgtcgctgct
      541 ccagcatccg ctccccccag atgtactgac ccccgtgctc gacaagatcg gagggaaccc
      601 tctctatgcg gaggaattca cgcgcctgct ccaggaccgc gggctgctgg ctcttgaaga
      661 cgggcaactg cgcctcaggc acggcgagga gttgccagtc ccggcgtcgc tccgagcagt
      721 cctcgcagcg cgtcttgacg gcctccctgc gctgcacaag gaacttctcg gggacgcggc
      781 cgtcatcggg gacaccttct ggcgcggggg cgtcgcggag atctctgcca ggagcgccga
      841 gatcgtggaa tcggtcatgg gctcgctggc ggagcatgag ctcgtccgtt ctcttgcgac
      901 cccgtccatc gcaggtgaag cagagtacac gttctggcat gcgctggctc gtgacgtcgc
      961 gtactcccag ttgccgcgca aggctcgggc gacaaagcac gaggcagctg ccgcctggat
     1021 cgagcggcgg gcagggccgc acgcatccga cttcgccgac atgctcgccc accattactg
     1081 ctccgccctt gatctgctcg gctcgatcgg tgagacggag tcagccgatc gcctggtcga
     1141 gccggcgatc cgctacctgg tgctcgcagg tgaccgcgcc atgaacctcg atgtggcagt
     1201 tgccgagcag cgctatgcca aggctctcga gctggccggg tccactggcc ctgagcgctt
     1261 tcgcgtgctc gccaagtggg ccggtgcgct ggccatggaa gacagggatc tcgaggccgt
     1321 tcgcgcctat cgggatgccg ttgagggcct catcggctcg ggagacgtgg gtgcggccgc
     1381 cctggcgctc tgccgcctca gtcggtgtct gtcgtcgctc ggcgacccga ctgcgcctga
     1441 cctgcccgcc caggcggtcc gactcatcga ggacatggag ccctcatccg aacaggtctt
     1501 catctacgcg gtgtacgcac aagtgctctt catctacggt gcgagtcatc ctgagacggt
     1561 gatcgaggcg gcgacgcgag cactcgaggt cgcaggcgcg ctcgggctgc ccgcaccagc
     1621 ggaggccctg ttctggcggg gctatgcgcg cctcgacctg ggtgaccttg ccggcctcga
     1681 ggagtgtgag cgggccgtgc ggttggcgag cgatcagggc ctcgggcaag agagagcccg
     1741 gatcgagcaa atgaatgcca tcttggccct gcgcgtgaag ggtccacgcg aatccgtggt
     1801 cgcctgcgat cggggtcttg agttctcccg gcgccgcggg cttgaggcct tcgtcgtcga
     1861 attccgcatg tcccggatcg aagcgctttg cctggctggc gagtgggact acgcgctcgc
     1921 tgagatccga gacctggtac ctgtgctcga ggaggccgag gacacctggc acctcctggg
     1981 cgtccggatc tcggaggcgc tcgtgctggc gcgacggggg cttccgtccg gcgccgccgg
     2041 gttcatcgag tggcttgaga gacaggggcg ggaccatcgc gccgctcaga tgagggcggg
     2101 cgcgttgctc gccgcagcgg ctgtccgcag cgcggaggga agggatgaag tgacgctgca
     2161 tctggtcgct gaattcctga gagtcaccga agaggcctcc gcctaccttg gtgtgctcga
     2221 ggacatgcca ggagtcatgc gttctgcggc cgcgtgcgga ggggcccagc acgccatcag
     2281 tctcgcccgg aggaccgcgg ccctggtgcc ggtgcagcgg ctgccgctgg accatgccgc
     2341 cgcgatgacc gtggcggccc tcgcagacga gctcggcggt gatgtgcgga cagcagccgc
     2401 gaaccatgcc gaagcggccg tcgcttggca tgacttgtcc gtgccttatg aggaagccca
     2461 ggcgctgctc ggtcaggggc gctgcctgat gacgctcgac aggccagacg aggcactgga
     2521 gcagttcaat gccgctcgcc cgatcttcga acggctcgga gccgagccgg ccttggccga
     2581 aacggatgag cagctgggaa gagccgctcg gtcttgagcc aggagcagcc acgcaatccg
     2641 ctccgccgtc tcctccagga ccactgccac gcgtcggcga cctgacctgc aagaccttca
     2701 tcaccggttc gactcccgtc gtcgcctcca acgacttccc tgcacctggt gattgcccac
     2761 tgcggccgcc gcgatgtgga gccacctcgg cccggcgtgg aatcgcgtcg cactggtaga
     2821 gttggcccgt gaaggagccg cgtctcatca ttttggggac gggatcagaa ggccgtgact
     2881 cggcgacggt cggtggcgac accgggacgt gccggcgctg aggcgccttc gcggcaaagg
     2941 aggaacagat gcccagcgtc tccctgcacc agaacgcccc tgagttcagc ctgctcgact
     3001 tctcgggcag gccgttcagc ctgctggagt atcgaggcga gaaacacgtg ctgctcgtct
     3061 tcaaccgcgg cttcacctga ccgttctgcc gacggcatat ggcgcagttg cgccgtgact
     3121 actccgagtt cctggcccgc gacgtcgaag tcgtggtcgt cggccccgaa gatgcatctg
     3181 ccttcgccgc ctactggcag aaggagtccc tgccgttcgt cggcctgccc gatgccaggc
     3241 acagcgtgct caagctctac gggcaggagg tgaaactctt caagatgggg cggatgccgg
     3301 cccaggtgtt gatcgacaag gccggcgtgg tgcgcttcgt gcactacgga cacagcatga
     3361 gcgacattcc cgagaatgcg gagatcctgg ctctggtgga cgagatcagc gccgcaccag
     3421 acccggtcgc ctagcggccc cgcagccctc cttcaaggct cgacctcgtg cgtcgtcctt
     3481 ctgacggcgc cgaggtggtt cgcgatcact catggggaag gcgcaggacg tgggtggcgg
     3541 tgtcgatctc gtgacgtctt cttcccgacg cagtgacgtc ggacgcgctg ctgtcgcctg
     3601 gtcggacaac agagatgcat gcggcggcgc cgatgggagt cgatagggtg ggtacggaac
     3661 atcgaacgtg agggaggttc gccatgcgcc ggctctggat cgtcgtcgcc atcgtgtgcg
     3721 gcatgcttgc cctcgccagt atggcatcgg cctcgatgcg cggtgaggtc cgttcgttcg
     3781 tcgctgcggt ggccgccgtg atgcccgatg gcgatgtgac cgcctcgccc tcgccggagc
     3841 ccagcctcag cgcgacagag tcagccatgc cgtcccccgc gccgagcgct gcgagcgccg
     3901 aggaggatgt cgatgagacc ggggctcagg gcagccacgg cacggccgtc tcaacggtgg
     3961 ccagggacaa ggatgcggtg ggcagcaaga cgctcgccaa cggcaagacc atcaccaacc
     4021 acggcatggc cgtctcggcc gtggcccgcg ccaaggcggg cggcggcgat ggtcactccg
     4081 gcggccaggg gaaggggaag gccggacact gacccccagg gcggagcctc gcgtcggcga
     4141 ccctgccggt caacgctgct gtgcccgggg cgctcggaga tcaccttcgg gtgaattgcc
     4201 tgcctgcagc gccagcatcc gccggtggtg tgtcgtatcg cgcttcttcg cggtcgtcgc
     4261 tgtctgctgc tgcttgtctc cgtctgcacg gcggcgccgg cgggagcgcc gcgacgggtg
     4321 acacgcaggg gggcggagac gtcttccgtc tcgccttcac ccttttcggg tgatgccgcc
     4381 cctgcgccga catggcagca tccaaatcgg gtgacccttg ctccggggag ggtcgtgctg
     4441 cctgcaccag cgatcacagg gaggggatca tgtcgcgcgt ccatccattc caggtcttcg
     4501 tctgggctgt gcttgtcctg agcgcgttgc tcgtcgtgcc cgctacggcc gcggccctcg
     4561 acggctcacc ccctcgcgtc tacctgcagg ccgatcccgc agttgccgac aatggtcctt
     4621 tcggttacgg agtgtggagc acgaagtcgg tcttcctcga ggtcgtcgcc agcgaccccg
     4681 agtccggcgt gagcaagatc gagcacgcga tcgccgtgcc ggacagcgag gagttcgtgt
     4741 ggctggaggg gtcgaagttc tcggtcgccg atgagggggt ctggtgggcg gtggcccgcg
     4801 cgaccaacgg tgacggcgtg accggtgtct tcctggccga cgacctggct cccaaactgt
     4861 ggaccgggga gtgggtcagc atcgacagaa cggtgccgac gaccgtcgcc agcggcgcag
     4921 tcagtgggca ctggtacgga accccggtca cggtgaccct ggcggcgagc gacggcgccg
     4981 gcggctcggg cttcctgatc accgagtaca ggctcgacgc aagcgggtgg actgcgggaa
     5041 gtgacgtccc ggtctcggct cagggcaagc acacgttgcg ttaccgctcg agcgacctgg
     5101 cgaacaacat ggaggccggc aagacactca ccttcggcat cgacacgcgc cggccgaaga
     5161 ccaaggctcc gtacgcggcc ggcgccgccc gcggcgcgat cgcgacgctc aagtacaagg
     5221 tcgccgaccc ccggccgggg agcccgacgg cggaggtcac gatcaaggtg atgaaccgtt
     5281 ccggcaagct cgtgaagacg ctcgggccct acgcgggcag agccgtgaac accctgctgt
     5341 ccgcgcgctt cacggtgccg cgctcgtgga gcgcaggcgc gtaccgtttc gccgtctctg
     5401 cgaaggacaa ggccggcaac acgcaggtgc tcccggccgg ctccaacagg ctggtcgtga
     5461 ggtagtcacg gcggcgtgat gagcgaggcg tacgcactcg cgccgtccgt tctgccgtag
     5521 ccagcacggg catcgcccgt acgccgtgcg cgccgccggg cccggatgtc gctcagccga
     5581 ttgccgacgg ccggcagaca agaagctcgg cagccgagct cgtgcgggcg gcccaaggta
     5641 gcgggcctca ttggcctggc aacgctccca tcagctacgg cttgggctac ttcaccgtga
     5701 accggcgcca ggtcgtcacg gcggcggcgg tgtctggcgt cgcctcgatc atggcgcgga
     5761 tgcggaagag accgcgctgc ctcaccgtga acgtccagct gtaggcaccg gtggcgccga
     5821 tcttctcgaa gtggtccctg acatccgccc acgtgcggcc caccctcctc tgccacttca
     5881 cgaagacgtc ctggccggcg agatcgatgg gcgccaccgt gccgctcacc ttgagcttcc
     5941 gtccgagagc gatcgacccg ttggtgaggc cgctcagctt gagcgtcagc ctcggcttcg
     6001 gcaagaggac ggccgccgcc ggccgccaga tgctgctcga tccgaagtcc ggcgtgagga
     6061 cggcgccctg ggagatggcg cggtaggctg cgaacggcac cggagacggc accgtgtaac
     6121 tgtacgcgcc ttcgctgaac atcccggcat cggtggtcac ctgagtcagg tctgtgaact
     6181 cggtgtcggc ccagagccgc tgctgcaggt agacggtgac gtcggggagc ccgaccttca
     6241 gcatgtagga cccattggga ccgtcgatga ggttgagcgc gcccggctgc cagagcttgc
     6301 ccgccagcac aagcccggcg gcggacgact tcccgctgag cgacagaccc gggatcctcg
     6361 gtgacgccgg atgcgggctc atgccgcggt ggcaggtgat gcagtcggtg acggcggcca
     6421 cgaggacgcc gtggggagcc ggtgggccgt cgggcggcac ggcatgacag tccacgcagg
     6481 tcgccgccgc cgcgggcgag gcgccgactc cgagcagagc gatcatgccc agacaaagga
     6541 ccactgccaa cactggtctg cgtctcatgg acccctcctg tctcgtcgcc tccccgaggc
     6601 gtgtctcaag cagtacgtct caagggtcgt atgaagacgg tctacgcccg ggcaccgggg
     6661 agttcaagcg tttgtcatcc gctcagaaga cggcggatcc gcgtcgtgtg tcaggcgcgg
     6721 agccttgcac ctccctggct ggtcggctag cttgccacca tgggctacac ggtcttccgt
     6781 cccgaggagc gcgcgttcga accgccgcag cgcggtgacc agcggcgggg ccgcgcgtcg
     6841 ctctccgagg ctctgcacga gatgcgcgcc aacatctggc gcctgccggc cggcgcgcgc
     6901 ggtacgcgtc acctcgagcg cgtccaggag gagatgttcg tggtcctcga gggcgccgcg
     6961 acactcctcc tcggcgaccc gcccgagcgg gtcgagctcc cacgtggttc cgtcgcggtc
     7021 gtcgagacgg ggaccgccct gcagctcagg aacgagagcg atgcggaggt ggtcgtcctc
     7081 atcgtgggcg ctcctccggt gcagggcgag gccgagtacc tgcccgacgt ggactgatgc
     7141 acggaagagc tattgagcgg ccgccggccc gaagggcgtc gcggcggacg gactagggga
     7201 tcgtgggaca cgcgacgagc ttggggggcg ctcatcagga cctggcgaag cgcctgcagc
     7261 gagggccggt cgccatggtc gagccgcggg atccggctgc gcggcaggcc tggcgcgaga
     7321 tcctcgagac gctcttctcg cccgaggacg ccgccctggc tgcccgcctg ccggtcgttc
     7381 ccaccaccgt cgccgacctt gcccggcgca ccggcttggg cgcgggcgct ctccgcgccc
     7441 gcctcgacgc gatggccggc aagggcctcg tccttgacct gcccgacgcg cggaccggcg
     7501 cgacgacgta cctgctcgcg ccgcccgtgg tggggttctt cgagctctcc atgatgcgct
     7561 tgcacgacgg cctgccgaag gcgcggctgg cccgggccta cgaggcctac atggccggcg
     7621 acagcgcgtt cgacgcggag gtctcgggga tccccacgct catcagccgc acgctcgtgc
     7681 aggagaccgc cctctacgac gacctcgcct cggaggtcct cgactgggaa cgagccacct
     7741 cgtatgtgga gagggccgac tgcctggccg tcacgaactg cttctgccgc cacactgccg
     7801 cgcatctcgg caccgcctgc gactatccga tggagacgtg catgagtctc gggacggcgg
     7861 cccgctacct cgtccggcaa ggccacgccc ggcaagtcgg cagggaggag gggcgcgagc
     7921 tcctcgaggc cgcccgggag cacggcctcg tgcacatcgg cgacaacgtg cggcgcgagc
     7981 tgaactacat ctgcagctgc tgctcgtgct gctgcgtcga gctgcgctcg gcgcagaagg
     8041 gcatgccggt gatgcagccg agcgccttcc agccggcggt cgccggtgaa cgatgcaccg
     8101 g
//
LOCUS       DASUMX010000224         6465 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1085877, whole genome shotgun sequence.
ACCESSION   DASUMX010000224 DASUMX010000000
VERSION     DASUMX010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 6465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6465
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1085877"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="VFZ86_10600"
     CDS             <1..427
                     /locus_tag="VFZ86_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642767.1"
                     /translation="FLRAVPSLLRGEPFRPEGSRGILDETLAQGFGVLADDADREIVI
                     GAYCRPWLADVEFRPLAPEEFASFDEPGFVKIAVSLAAEPLGEDSSRFVTRTRAVATD
                     AEARRKFRRYWSRMSAGIILIRWFFLPLLKRQVERQAST"
     gene            671..2062
                     /locus_tag="VFZ86_10605"
     CDS             671..2062
                     /locus_tag="VFZ86_10605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX5642768.1"
                     /translation="MIRALRARERFLTGIMGSLESFFTIDAQWRCTFANEAGVALLGV
                     AEEQILGRDVRELLPDDLREKVSAQLDLAMSGRRAVTLRSKDSRHGPPMHEAIAYPVS
                     DGGLAVYIRDVTRLELAEADAVEAERRYEELVENVNSAILRWTRDGSLTFFNKYAERL
                     FGWRADEVLGRHVSFLLPESDEGGEGLSGLAEDIVAHPGRYANNVNENVCKDGSRLWM
                     AWTNRALLDERGEIREVLAVGNDVTALLAAQAALREREEERIAQEERNRLARDLHDSV
                     TQALFAATMKAEALTLATDALSAENVQAVEDVRRLNRGALAQMRTLLLELRGDPLEEV
                     PLQPLLRQLAEAAEARSSATVLLTIRGDGKLPPDLHVAVYRITQEALTNVTRHAKASK
                     AWVDLDLKPGEVHLVVGDDGRGFDPSTVGTAHLGLKSMRERAALVGAELDILSTSHAG
                     TVVTFGWSTRKRH"
     gene            complement(2101..2736)
                     /locus_tag="VFZ86_10610"
     CDS             complement(2101..2736)
                     /locus_tag="VFZ86_10610"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5642769.1"
                     /translation="MGVPEFFADQLRNPTGFFGRFVMSRFFDRSSAAINQLTMRSLAL
                     EPADRVLEVGFGSGDLIAHVAPVVREGFVAGVDFSPDMVSVATKRLAALVSAGRVELR
                     CAGAERLPYDEGSFTKACTVNTVYFWPEPAVAMKELARVVGPGGRLVIGFSPAEAMRR
                     MPKRLTEHGFTLFEPEDMRHLFEDAGFGAIEMVPGHGPRGDFLCAIGTRSA"
     gene            complement(2858..3418)
                     /locus_tag="VFZ86_10615"
     CDS             complement(2858..3418)
                     /locus_tag="VFZ86_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX5642770.1"
                     /translation="MRPLRYSINVTLDGCCDHREGVADEELHRHAAENLGRADALLFG
                     RVTYEMMEAAWRPPAPAGARPEWMQPWMVPFARSIDAAKKYVVSSTLERVDWNAELVR
                     GDLGKAVRQLKREPGKGLLTGGVMLPLALAELGLIDEYEFVVHPRIAGHGPTLFAGLS
                     QRIDLKLVGRLELGSGAVALRYEPRR"
     gene            complement(3446..3889)
                     /locus_tag="VFZ86_10620"
     CDS             complement(3446..3889)
                     /locus_tag="VFZ86_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015460307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEX5642771.1"
                     /translation="MADRATKGSGDAAAPVLLSGGNPQIAKADGDAPVQAYIAAMPGW
                     KSEVGRRLDAIVARTVPDVRKAVRWNSPFYGVEGQGWFLNFHCFTKYVKVAFFNGTSL
                     EPLPPGESKDEHARYLTIYEDDQFDEQQFADWVGQASRLPGWGTS"
     gene            4097..4693
                     /locus_tag="VFZ86_10625"
     CDS             4097..4693
                     /locus_tag="VFZ86_10625"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5642772.1"
                     /translation="MGRGEAGAAEAAVQVGVMAPGSEQADVLLRAARTGDPDAFMELL
                     APERSRLESLARQLLGDRADVADVLQEVYLSAYRALPKYRGDSRLATWLYRITYNACL
                     RHQARRPETGGADVEEGPPSPDHAASVVARLDLAAALAELPIEQRALVLMIDRDGFGY
                     RAAAEALSIPLGTVSSRLAAARAKLRDALAPDEEGGGR"
     gene            4690..5781
                     /locus_tag="VFZ86_10630"
     CDS             4690..5781
                     /locus_tag="VFZ86_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642773.1"
                     /translation="MNRDHDELVDEHRDRELGAALAALPHPELPDDMDARLRAALEVE
                     HRRRRGRRSIATLGLAATLAAIALASAALAGAFDSSSPAPWPEPPLPAESVYPTNAAG
                     ETYGANKPLVERPDLMAAVNRQGVHGYIRRSDMEPPPGSPEEAEQANKDSLRGYTIPL
                     YEADGVTQIGVFQVGGPGSGSGGTMADGTKFEQTSDEDGNIITTTTHPDGSVTIATEA
                     LDGTVTTQELTAAEAKRLRQETAATPKPTTTPTRGPDKPRDWLLRRMSQLARDAGDAH
                     ATAWWELQSRYYLKPIEGDKTPESPYQQWASVWLVILHGDFAGGDWRYWLLDQDSHNV
                     LSSGQSDTRFVMSGPQLPPPQGPITLGGE"
     gene            complement(6135..>6465)
                     /locus_tag="VFZ86_10635"
     CDS             complement(6135..>6465)
                     /locus_tag="VFZ86_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="spore germination protein GerW family protein"
                     /protein_id="HEX5642774.1"
                     /translation="KAVFGEPVEKDGVTIVPVARVRYGGGGGGGRGPGRKKKGDAGDA
                     DQVGFGHGGGVQAAPVGYIELSGGQATYRRIADPARPMAVALLFPLVGAIAVAIVALS
                     SRLRSRG"
BASE COUNT         1010 a         2278 c         2245 g          932 t
ORIGIN      
        1 cttcctgcgg gccgtgccct ccctgctcag gggcgaaccc ttcaggcccg agggctcgcg
       61 gggcatcctc gacgagacgc tcgcccaggg cttcggggtg ctcgccgacg acgccgacag
      121 agagatcgtc atcggcgcat actgccggcc gtggctcgcg gacgtggagt tccggcccct
      181 ggcgccggag gagttcgcgt cgttcgacga accggggttc gtgaagatcg cggtctccct
      241 cgctgccgaa ccgctcgggg aggacagttc gcgattcgtc acgcgcacga gagccgtggc
      301 gacggatgcc gaggcgcggc gcaagttccg ccggtactgg tcacggatgt cggcgggcat
      361 catcctcatc cgttggttct tcctccccct gctgaagagg caggtggagc gtcaggcctc
      421 cacctgaact ggtcctcgca ggcattcggc aagccgcgcc acagactacc cgcactggct
      481 cgcggcctcg ctgccgacgc cggcttgccg acaagcacta ccgggtagcc tgacagcgga
      541 gcctcgtctt tgtgagccca gcggacccta gactttgagg gtctttcccc cgaacgcaga
      601 ggaccatcgt ttgccggacg tcgtgcccga gagccgtgcc gagcttgtga gcgagcttga
      661 tcgcgatgcg gtgattcgcg ccctccgggc gcgagaacgg ttcctgaccg ggatcatggg
      721 cagcctcgag agcttcttca cgatcgatgc gcagtggcgc tgcaccttcg ccaatgaggc
      781 gggagtcgcc ttgctggggg tggcagaaga gcagatcctc gggcgggacg tgcgggagtt
      841 actccccgac gacctacgcg agaaggtctc tgcgcagctg gacctcgcca tgagtggccg
      901 cagggccgtc accctccggt ccaaggattc gaggcatggc ccacccatgc acgaggcgat
      961 cgcctatccc gtgagcgacg gggggctcgc cgtctacatc cgcgacgtca cacgactgga
     1021 gctggcggaa gcagatgcgg tcgaggccga gcggcgttac gaggagctcg tggagaacgt
     1081 gaacagcgcg atcctgcgct ggacacggga cggcagtctg accttcttca acaagtacgc
     1141 cgagcgcctg ttcggctggc gcgcggacga ggtcctgggg cggcacgtca gcttcctgct
     1201 ccccgagagc gacgagggtg gcgagggcct ctccggactc gccgaggata tcgtcgctca
     1261 ccccggccgc tatgcgaaca acgtcaacga gaacgtgtgc aaggacggca gccggctgtg
     1321 gatggcgtgg accaaccgag ccctcctcga cgagcgcggc gagatcaggg aggtgctcgc
     1381 cgtgggtaac gacgtcaccg cgctcctcgc cgcccaggca gccctccgcg agagggagga
     1441 ggagcggatc gcgcaggagg agaggaaccg cctggcgcgc gacctgcacg actcggtgac
     1501 gcaggcactc ttcgccgcga ccatgaaggc cgaggctctg acgctggcga ccgacgccct
     1561 gtcggccgag aacgtccagg ccgtcgagga cgtgcggcgg ctgaaccgcg gcgctctggc
     1621 gcagatgcgc acgctcctgc tcgagctgag gggggacccg cttgaggagg tgccgctcca
     1681 gccgctgctg cggcagctgg cagaggccgc ggaggctcgc tcgagcgcga cagtcctcct
     1741 gaccatccgc ggggacggga agctgccgcc tgatctgcat gtcgcggtct accggatcac
     1801 gcaggaggcg ctcaccaatg tgacgcgcca cgccaaggcg tcaaaggcgt gggtggacct
     1861 ggacctcaag ccaggcgagg tccatctcgt cgtcggggac gacggtcgcg gtttcgaccc
     1921 ctcgaccgtc ggaacggccc atctcggcct caagtcgatg agggaacgag ccgcactggt
     1981 gggcgccgaa ctcgacatcc tgtcgacgtc gcacgccgga acagtcgtga cgttcggctg
     2041 gtcgacgcgt aagcgacact gaagacgcgc tgccgtggcg gagctgctcc tgaggtcgtt
     2101 ctaggcggac ctcgtcccga tggcgcagag gaagtcgccg cgcgggccgt ggcccggcac
     2161 catctcgatg gcgccgaagc cggcgtcctc gaacagatgc cgcatgtcct ccggctcaaa
     2221 gagggtgaag ccgtgctcgg tcagtcgctt cggcatcctg cgcatggcct ccgcggggct
     2281 gaagccgatg acgagcctgc ctccggggcc caccacgcgc gcgagttcct tcattgcgac
     2341 ggccggctcg ggccagaagt agacggtgtt gaccgtgcac gccttggtga agctcccctc
     2401 gtcgtagggc aggcgctcgg cgccggcgca gcggagctcc acccggcccg cgctcaccag
     2461 tgcggcgagg cgcttggtgg cgaccgagac catgtccggc gagaagtcga cgccggcgac
     2521 gaagccctcg cggacgacgg gagccacgtg ggcgatcagg tcgccggagc cgaaacccac
     2581 ctcgagcact cggtcggccg gctcgagcgc cagcgagcgc atggtcagct ggttgatcgc
     2641 cgcgctggag cggtcgaaga accgcgacat cacgaagcgg ccgaagaacc ccgtgggatt
     2701 ccgcagctgg tcggcaaaga actcaggcac tcccatggac tcccctcccg gtggactcag
     2761 ccgagtctag ttccccgcgg gctcctgaaa caggccccga cggccgggcg tcgtgcggct
     2821 ccgatgcagc cgactcgggc tgccaggccc gatgtctcta cctcctcggc tcgtagcgca
     2881 gcgcgaccgc ccccgagccg agctccagcc ggccgacgag cttcaggtcg atacgctgag
     2941 aaagcccggc gaacaacgtc ggcccatgac ccgcgatcct gggatgcacc acgaactcgt
     3001 actcgtcgat caatcccagc tccgccaagg ccagcgggag catgacgcct ccggtcagca
     3061 gtcccttgcc aggctcccgc ttgagttgcc gaacggcctt ccccagatcc ccgcgcacga
     3121 gctccgcgtt ccagtcgacc cgctcgaggg tgctcgacac gacgtacttc ttcgccgcgt
     3181 cgatggaccg ggcgaagggc accatccagg gctgcatcca ctcaggcctt gctccggccg
     3241 gcgccggcgg ccgccacgcg gcctccatca tctcgtaggt cacccggccg aagaggaggg
     3301 cgtcggcccg gcccaggttc tcggccgcgt gacgatgcag ctcttcgtcc gcgaccccct
     3361 cacgatgatc gcagcacccg tcgagggtga cgttgatgga atagcgaagg ggtcgcatct
     3421 caccgcgagc ctgtgcgatg cgtcgtcacg acgtgcccca gccgggcaac cgactcgctt
     3481 gcccgaccca gtcggcgaac tgctgctcgt cgaactgatc gtcctcgtag atggtgagat
     3541 agcgcgcgtg ctcgtctttg gactcgcccg gcgggagcgg ctccagcgac gtcccgttga
     3601 agaacgccac cttcacgtac ttcgtgaagc agtggaagtt gaggaaccag ccctgcccct
     3661 ccacgccgta gaagggcgag ttccagcgca ccgccttccg cacgtccggg acggtgcgcg
     3721 ccacgatcgc gtccaggcgg cgtccgacct cgctcttcca gcccggcatg gccgcgatgt
     3781 aggcctgcac gggagcgtcg ccgtctgcct tcgcgatctg cgggttgccg cctgagagga
     3841 ggaccggcgc ggctgcgtct ccagacccct tggtcgccct gtccgccatt gctcacactc
     3901 cctgccggtg ggtctaccga tggtgtggtg attcccctgg cgggttcatc gctaccggcc
     3961 gggtcaggcg ttggaggtag cgcgcccgcg cttgcggcac tccctcgcgt ctgattgccg
     4021 cgaccgaaca gaacggactc cgcgcgtatc taagtagagt gtgagtgggt gaagagcact
     4081 ccgcgaagga gacgtcgtgg gtcgaggaga ggcaggggcg gcggaggccg cggtgcaggt
     4141 gggggtgatg gcccccggct ccgagcaggc ggacgtgctg ctgcgcgcgg cccgcacagg
     4201 cgaccccgac gccttcatgg agctgctcgc tccggagcgc tcccgcctcg agtcactcgc
     4261 gcgccagctg ctgggcgacc gggccgacgt cgccgacgtg ctgcaggagg tctacctctc
     4321 ggcgtatcgt gcgctgccca agtacagggg cgactcccgc ctcgccacct ggctctaccg
     4381 catcacgtac aacgcctgcc tgcgccatca ggcccgccgc ccggagacgg gcggcgcgga
     4441 cgtggaagag ggaccgccct cgccggacca cgccgcctcg gtggtcgcgc gcctggacct
     4501 cgcggccgcg ctggctgagc tgcccatcga gcagcgcgcc ctcgtgctga tgatcgaccg
     4561 cgacggcttc ggctaccgtg ccgccgccga agcgctctcg attcctctgg gcacggtgag
     4621 ctcgcgcctc gcggccgcga gggccaagct gcgcgacgcg ctcgcgcccg atgaggaagg
     4681 gggcggtcga tgaaccggga ccatgacgag ctcgtggacg agcaccgcga tcgcgagctg
     4741 ggcgcggcgc tcgccgccct gccgcatccc gagctgccgg acgacatgga cgcccggctg
     4801 cgggcggcgc tcgaggtgga gcatcgcagg cgccgcgggc gccggagcat cgccaccctc
     4861 ggtctcgccg ccacgctggc cgcgatcgcg ctcgcaagcg ccgcgctggc cggcgccttc
     4921 gactcgagct cgccggcgcc gtggcccgag ccgccgcttc cggccgagtc cgtgtatccg
     4981 acgaacgccg ccggcgagac ctacggcgcc aacaagccgc tcgtcgagcg gcccgacctc
     5041 atggccgcgg tgaacaggca gggggtgcac ggctacatcc gcaggagcga catggagccg
     5101 ccgcccggct cgcctgagga ggccgagcag gcgaacaagg acagccttcg gggctacacg
     5161 atcccgctct acgaggccga cggggtcacg cagatcggcg tcttccaggt cggcggtccc
     5221 ggcagcgggt ccggcgggac gatggccgac gggacgaagt tcgagcagac gtccgatgag
     5281 gacggcaaca tcatcacgac gacgacgcat ccagacggca gcgtgacgat cgcgaccgag
     5341 gcgctcgacg gcacggtcac gacccaggag ctgaccgcgg cggaggcgaa gcggctcagg
     5401 caggagacgg cggcgacgcc gaagccgacc accactccta cccgcggacc cgacaagcca
     5461 cgggactggc tgctcaggcg tatgtcccag ctcgctcgcg acgccggcga cgcccacgcg
     5521 accgcctggt gggagctgca gtccaggtac tacctgaagc ccatcgaggg cgacaagacg
     5581 ccggagtccc cgtaccagca gtgggcaagc gtctggctcg tcatcctgca cggcgacttc
     5641 gccggaggag actggaggta ctggctgctc gaccaggatt cgcacaacgt cctctcctcg
     5701 gggcagagcg acacacgctt cgtcatgtcg ggcccacagc tcccgccgcc gcaggggccg
     5761 atcacgctcg gcggcgagtg acgcagcgga ggcgccatcc gagacggtcg cgagacgcct
     5821 gaatctcatt ggtggccgct cgggcgaagg ctgtggccgg gctcgatgag gtcacgcgcg
     5881 cagcacttga ggccgccgag gacacggccg gcgaggatgc gcggcccgcg cccgacaacg
     5941 tcatcgctct cgccgctcga aggcgtcagc tcctgcagtt ccttcgcttc gggctgctgg
     6001 gccgcgcggg cgggtctcgg agtgacctcg tttcaggccg cgcctcttgc cttgcggagc
     6061 tctcgcgcca gtgctctgtg aaggtcgacg gggacgacgg cgacgtcctg tggcacgcgg
     6121 tcggcgcggc gacctcagcc ccgcgacctg agccgcgacg agagcgccac gatggcgacc
     6181 gcgatcgccc ccacgagcgg gaagagcaac gcgacagcca tggggcgtgc cgggtcggcg
     6241 atgcgcctgt acgtggcctg gccgcccgac agctcgatgt agccgacagg cgcggcctgc
     6301 acgccgccgc cgtggccgaa gccgacctgg tctgcgtcgc cggcgtcgcc cttcttcttg
     6361 cgtcccggtc cacggccccc gccgcccccc ccgccgtacc gcacccgggc gacgggcacg
     6421 atggtcacgc cgtccttctc gacgggctcg ccgaacaccg ccttg
//
LOCUS       DASUMX010000225         8766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1518259, whole genome shotgun sequence.
ACCESSION   DASUMX010000225 DASUMX010000000
VERSION     DASUMX010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 8766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8766
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1518259"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..179)
                     /locus_tag="VFZ86_10640"
     CDS             complement(<1..179)
                     /locus_tag="VFZ86_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HEX5642775.1"
                     /translation="MTGQRQSGATAGKPALVAIVGKSDSGKTTLIEKLVPELVKLGLR
                     VGTVKHDAHSFEIDHP"
     gene            complement(223..1281)
                     /locus_tag="VFZ86_10645"
     CDS             complement(223..1281)
                     /locus_tag="VFZ86_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014987327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5642776.1"
                     /translation="MSLEAAVGLRKGTLDLDVRLEVRSSGVVVLLGPNAAGKTTLLRA
                     LAGLVPLERGRVTLDDAVLEDTTAGVRTPTERRPIGVVFQDYLLFPHMTVLENVAFGL
                     RARGTGRALARARALEMLERVGLSDRASAKPRALSGGQAQRVALARALATDPRLLLLD
                     EPLSAMDAGARAELRRGLSRHLAAFPGTCLVITHDPIEAMTLGDQLVVLEAGRVVQAG
                     TPEELSSRPRSRYVADLLGLNLYHGRADERSIVLTGGRRLIAADPVPASGEVFAVIPP
                     RAVALHRVLPEGSPRNVWKGVVEDLDVVGNHVRAHVSGELPIIAEVTPGAVASMRLDD
                     GGPVFVAVKAVEVEVYEA"
     gene            complement(1316..2311)
                     /locus_tag="VFZ86_10650"
     CDS             complement(1316..2311)
                     /locus_tag="VFZ86_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019526121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5642777.1"
                     /translation="MNSRRRQAVERGTGGPPLRDGDHTGGPAGGRSRETAGGLAVVAG
                     DAIAPPPRPEPPPWSAPAPATTRAARRGDERAPLGFVPLAAVAVAFLTLPLVALLVRA
                     PWGSLPGDLTAPGVLAALRLSLICSVGALGLSLLFGVPLAWLLARVPFRGRNLVRALV
                     LLPMVLPPVVGGVALLLAFGRNGIAGQWLDSLAGVTLPFTTLGAMLAETFVAMPFLVI
                     TVEAGIRAMDRRYEDAAATLGAGRWRTFRLVTLPLIAPALAAGGALCWARALGEFGAT
                     ITFAGNLPGSTQTMPLAVYLALQNNPDAAITLSLVMLAISLAVLVGLRSHWFPSR"
     gene            complement(2308..3087)
                     /gene="modA"
                     /locus_tag="VFZ86_10655"
     CDS             complement(2308..3087)
                     /gene="modA"
                     /locus_tag="VFZ86_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018848272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX5642778.1"
                     /translation="MTRPTGRRSLAAVGTLLAALALVVCACGANADAGGERSRELSVF
                     AAASLTDAFTQLGDRFTAAHPGVTVTFNFAGSNDLVTQLRQGAPADVLATADTRSMEV
                     AGDLVDEPLAFAGNKLAVAVAPGNPEGIGGLADLARADLKVVLAAPEVPAGKYAEEAL
                     AKAGVSVAPVSLEVSVKGVVTKVSLGEADAGVVYVTDVAAAGEKLDGIAIADDHNVLA
                     TYPLAALVANAHPDDARAFIDFVLSPEGQQVLADHGFLPAP"
     regulatory      complement(3161..3295)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            3412..3699
                     /locus_tag="VFZ86_10660"
     CDS             3412..3699
                     /locus_tag="VFZ86_10660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642779.1"
                     /translation="MALLIIDHVVGDYETFKQVFLDDQERRRRLGSKSARVYRVDGDP
                     NNIRVVIEFETAELARAHAEGLELHEAIKWATGNVSMPQFEVLEPVMETDA"
     gene            complement(3721..4866)
                     /gene="mobB"
                     /locus_tag="VFZ86_10665"
     CDS             complement(3721..4866)
                     /gene="mobB"
                     /locus_tag="VFZ86_10665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HEX5642780.1"
                     /translation="MAPPPTDQDTVRVGVLLAGGESRRMGLDKRGLRLGGDTLLQRNL
                     AFLAGIFPVVCLSVRSAGQAPPDLPAGVVVIPDEVTGSPLAGLASVLGRLGEPVFAMA
                     ADLIAPDPGAVGRVLEAFRDADVALPVADDHLEPLHAVYGPDCLPYMRRLLAAGAHSI
                     LDLFPLVRVARVAFADTAPFFNVNTPADWAEAQRRLGVGPARGDAMELERPARGPGTP
                     AVLGVVGRPNSGKTTLIEQLIPQFTRRGLRVGAVKRVARFDIDVPGKDSWRHSRAGAA
                     AYTVASASKLAFVAQRDGEASLDEIVARYFGGYDVVICEGYRREAPDVVEVFRAGAGY
                     DSPVCEPAEALALVTDTELAHRRRFGLDDAAALATFLVERLDLEARA"
     gene            5063..7666
                     /locus_tag="VFZ86_10670"
     CDS             5063..7666
                     /locus_tag="VFZ86_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005031368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX5642781.1"
                     /translation="MGEVQRLTNCTTGGPVFVDVEDGRIVRMYPIDLADDDKGDWVIE
                     ARGRKFTPPRRTTLSPHAQAQRSMVYSPTRILHPMKRVDWDPEGERNTHNRGVSGYEE
                     ISWDEALDLVTTELIREKRENGPGSILTTCSSHHLWGNLGYRHSAYYRFLNLMGMVHS
                     AHNPDSWEGWHWGAMPMWGNSHRLGIPEQYDLLEDALKHTEMIVFWSSDPESTGGGIY
                     SAFESTSRRYWMKELGIKMVFVDPYYNPTAELFSDKWFAPRLGTDVAFGLGIAHVWLA
                     EGLYDKEYVAARTTGFDEWRDYVLGKTDNVPKTPEWAESECGIPARQIRALAREWGSK
                     KVMLAAGGLGGWGGACRSATGNEWARTMVALAAMQGYGKPGVNIWGTSQGAPSDCSFV
                     FPGYAEGGMSGDTEKSAAGFRMLYRIFKPETGLHPTVNAHDSPEGIAIPRLRIPEAMM
                     HEKFEWRGKGFCGPSIQSQMQKYQYPAPGYPFVTMYWRYGGSFFGTMQQTNRYVKAYR
                     EGGVRTVVNQSIWMEGEAKFADIILPACTNFERWDMSEFGNCSGYIPDNHTQTSHRVI
                     VFQKKCIEPLGESRSDYDIFAAVAGRMGMGDVFTEGGMDEYDWCKAYFDATDLPQITT
                     WEEFEQKGYVVVPIPDDYKSTPAMRWFAEGRKRDTPDWGPRPGDTVGFEGLQTPSGKI
                     EFVSRTLQHLELDVVDPERPAMGPQFIPSWEGHHTTELYGTYPLQMVSPHPRFSFHTM
                     GDSKGTWLNEVEEHRILHEDGHRYWIMRLNSVDAAARGIAEGDLIRAYNDRGSVILSA
                     QITERVAPGTVHSYESCADYLPTGEPGESTDIAGCVNILTNKRFITPTSIAMSNNSCL
                     IQVEKWEGEAS"
     gene            7663..8496
                     /locus_tag="VFZ86_10675"
     CDS             7663..8496
                     /locus_tag="VFZ86_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005037602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX5642782.1"
                     /translation="MKSWHLIVDVARCHDCNDCFLADKDEFVDNEFPPYSAAQPWHGH
                     RWMNIKRKERGQYPLVETAYLPMPCMHCDEAPCLTADGAVYKRPDGLVIIDPVKARGR
                     KEIVASCPYSAIYWNEEKQLAQKCTGCAHLLDDGWTETRCSQVCPTEAIKLVLAEEDD
                     VERMVEEEGLARYRDSLGTRPRVYYKNLHRWDKAFVGVSVVFGDSDECAEGARVSVSR
                     EGAATREGTTDIFGEVKIDHLTPGREYAVKIEADGYKAAELTVQLDASVNAGTVVLEK
                     A"
BASE COUNT         1374 a         3100 c         3170 g         1122 t
ORIGIN      
        1 gggtggtcga tctcgaacga gtgcgcgtcg tgcttgacgg tgccgacgcg caggccgagc
       61 ttcaccagct cgggcaccag cttctcgatc agggtggtct tgccggagtc gctcttgccg
      121 acgatggcga cgagggcggg cttgcccgcc gtcgcgccgc tctgccgctg cccggtcatg
      181 cgctgcagcg tagcgaagaa cccggcgggg cgaaacccgg gctcacgcct cgtagacctc
      241 gacctcgacc gccttgacgg cgacgaacac cggcccgccg tcgtccaggc gcatggaggc
      301 gacggccccc ggggtgacct cggcgatgat cggcagctcg ccgctgacgt gcgcgcgcac
      361 gtggttgccg acgacgtcga ggtcctcgac gacgcccttc cagacgttgc gcggcgagcc
      421 ctccggcagc acgcggtgca gcgccacggc gcgtggcggg atgacggcga acacctcacc
      481 gctcgccggc accggatcgg ccgcgatcag gcgccggccg ccggtgagca cgatcgagcg
      541 ctcgtcggcc cggccgtggt acaggttcag gccgagaagg tcggccacgt accgggagcg
      601 tgggcgcgac gagagctcct ccggcgtgcc ggcctgcacc acgcgccccg cctcgaggac
      661 gaccagctgg tcgcccagcg tcatggcctc gatggggtcg tgcgtgatca cgaggcacgt
      721 gccggggaag gcggcgagat gccgggagag gccgcgccgc agctcggcgc gggcgccggc
      781 gtccatcgcc gacagcggtt cgtccagaag gagcaggcgc gggtcggtcg cgagcgcccg
      841 cgccagggcg acgcgctgcg cctgccctcc ggagagcgcc ctgggcttcg ccgacgctct
      901 gtcgctcagc ccgacgcgct cgagcatctc gagcgcgcgt gcgcgcgcga gggcgcggcc
      961 ggtcccgcgc gcgcggaggc cgaaggcgac gttctcgagc accgtcatgt gcgggaacag
     1021 caggtagtcc tggaagacca cgccgatggg ccgccgctcc gtgggcgtgc gcacgccggc
     1081 cgtggtgtcc tcgaggacgg catcgtcgag cgtcacccgc ccccgctcca gcggcacgag
     1141 cccggccagc gcccgcagca gcgtggtctt gccggcggcg ttggggccga gaagcacgac
     1201 cacgccgctg ctccgcacct cgagccgcac gtcgaggtcc agcgtgccct tgcgcaggcc
     1261 gaccgccgcc tcgaggctca cgcctcgcct cccctgtcga ctgcgcccgc cccggtcatc
     1321 gcgacgggaa ccagtgactg cgcaggccga cgagcacggc cagcgagatc gccagcatga
     1381 cgaggctcag ggtgatcgcc gcgtcggggt tgttctgcag cgccaggtac acggcgagcg
     1441 gcatcgtctg cgtgctgccc gggaggttgc cggcgaaggt gatggtggcc ccaaactcgc
     1501 ccagggcacg cgcccagcag agagcgccgc cggcggcgag cgccggcgcg atcagcggca
     1561 gggtcacgag ccggaacgtc cgccagcgcc ccgcgcccaa ggtggcggcc gcgtcctcgt
     1621 agcgcctgtc catggcgcgg atgcccgcct cgaccgtgat gaccaggaag ggcatggcga
     1681 cgaaggtctc ggccagcatc gctcccaggg tcgtgaaggg cagcgtgacg ccggccaggc
     1741 tgtcgagcca ctgcccggcg atgccgttgc gcccgaacgc gagcagcagc gcgacgccgc
     1801 cgacgaccgg cggcagcacc atgggcagca gcaccagggc gcgcacgagg ttgcggccgc
     1861 ggaaggggac gcgcgcgagc agccaggcga gcggcacgcc gaagaggagc gagaggccga
     1921 gcgcgcccac cgagcagatc agcgacaggc gcagggcggc gagcacgccg ggcgccgtga
     1981 ggtctccggg caggctcccc cacggcgcgc gtaccagcag cgcgaccagc ggcagggtca
     2041 ggaaggcgac cgccaccgcg gcgagcggga cgaagccgag cggcgcgcgc tcgtcgcccc
     2101 ggcgcgcggc gcgcgtcgtc gccggcgccg gcgcggacca gggcgggggc tcgggccgcg
     2161 gcggcggcgc gatggcgtcg ccggcgacga cggcgaggcc tcccgccgtc tccctgctgc
     2221 gcccgccggc cgggccgccc gtgtggtctc cgtcgcggag cggtggtcct ccggtgcccc
     2281 gctcgacagc ctgtctacgg cggctgttca cggcgccggc aggaacccgt ggtcggcgag
     2341 gacctgctgg ccttccggcg agaggacgaa gtcgatgaag gccctcgcgt cgtccgggtg
     2401 tgcgttcgcg acgagggcgg cgagcgggta ggtcgcgagc acgttgtggt cgtccgcgat
     2461 cgcgatcccg tcgagcttct ccccggcggc cgcgacgtct gtgacgtaca cgacgccggc
     2521 gtccgcctct cccagcgaga ccttggtgac gacgcccttg acggacacct ctagggagac
     2581 cggtgcgacg ctgacgccgg ccttcgcgag agcttcctcc gcgtacttgc cggcgggcac
     2641 ctccggcgcc gccagcacca ccttgaggtc cgcgcgcgcg aggtcggcga gcccgccgat
     2701 cccttccgga ttgccggggg cgacggcgac ggccagcttg ttgccggcga aagcgagtgg
     2761 ttcgtccacg aggtcgccgg ccacctccat gctcctggtg tcggccgtcg cgagcacgtc
     2821 ggccggggcg ccctgtcgca gctgcgtcac caggtcgttg ctgccggcga agttgaaggt
     2881 gacggtgacg ccggggtgcg ccgcggtgaa ccggtcgccg agctgggtga aggcgtcggt
     2941 cagcgacgcc gccgcgaaga cgctgagctc gcgcgagcgc tcgccgccgg cgtccgcgtt
     3001 ggccccgcag gcgcagacga ccagggccag ggcagccagg agcgtgccga ccgccgcgag
     3061 tgaccggcgg cccgtggggc gcgtcacgac ggcgtgtgcc cggggccgag agtggtgggg
     3121 tgggcccggc ccccgtgggt cgagtgggtc atgtccctgg ctcctttcca agcacgggga
     3181 ggtccgggcg tttccgcccg ggcccacgac ggcgacccca cgtcgccgcc ccacggtctg
     3241 tccgtggtac ggcccggcgc ccgtagcggg atgccgtagg gcgaagggct tggagatgga
     3301 cgggcagagg gtaccaccgg gggcagggac gacgccagtc cggtggccac cggaacgccg
     3361 gcggcactgg agtatgattg cgccgcggat ccggcccgaa ggaggcggcg tatggcgctg
     3421 ttgatcatcg accacgtcgt tggcgattac gagacgttca agcaggtctt cctggacgac
     3481 caggagcgcc gccggcgact ggggtcgaag agcgcccgcg tgtacagggt cgacggcgat
     3541 ccgaacaaca tcagggtcgt catcgagttc gagaccgcgg agctggcacg cgcccacgcg
     3601 gagggactgg agctgcacga ggccatcaag tgggcgacgg gcaatgtctc catgcctcag
     3661 ttcgaggtgc tcgagcccgt catggagacc gacgcgtagt cacgcgccgg gcgccggagg
     3721 ctaggcgcgt gcttcgaggt cgaggcgctc gaccaggaac gtcgccagcg cggcagcgtc
     3781 gtcgagtccg aagcggcgcc tgtgggcgag ctccgtgtcg gtgacgagcg ccagcgcctc
     3841 ggccggctcg cacaccggcg aatcgtagcc ggcgcccgca cggaagacct ccacgacgtc
     3901 gggcgcctcg cgccggtacc cctcgcagat cacgacgtcg tagccgccga agtagcgggc
     3961 gacgatctcg tccaggctcg cctccccgtc gcgctgcgcg acgaaggcga gcttggacgc
     4021 cgaagccacg gtgtacgcgg cggctcccgc ccgggagtgc cgccacgagt ccttgcccgg
     4081 cacgtcgatg tcgaagcgcg ccacccgctt gacggcgccg acgcggagcc cgcggcgcgt
     4141 gaactgtggg atgagctgct cgatgagggt cgtcttgccg ctgttcggcc ggccgacgac
     4201 gccgaggacc gcgggcgttc cggggccgcg cgccggccgc tccagctcca tcgcgtcgcc
     4261 gcgcgccggc ccgacgccca gccggcgctg cgcctcagcc cagtccgccg gcgtgttgac
     4321 gttgaagaac ggcgccgtgt cggcgaacgc cacgcgcgcg acgcgcacga gaggaaagag
     4381 atcgaggatg ctgtgcgcgc cggccgcgag cagccgccgc atgtacggca ggcagtcggg
     4441 cccgtagacg gcgtggagcg gttccagatg gtcgtcggcg accggcagcg cgacgtccgc
     4501 atcacggaac gcctcgagca cgcgcccgac ggcgccgggg tccggtgcga tcaggtcggc
     4561 cgccatggcg aagaccggtt cgccgaggcg gccgagcacc gacgccagcc cggccagcgg
     4621 agagcccgtc acctcgtccg ggatcacgac cactccggcc ggcaggtccg gcggcgcctg
     4681 cccggcgctg cgcacggaca ggcagaccac ggggaagatg ccggcgagaa aggcgaggtt
     4741 gcgctgcagc agcgtgtcgc cgccgaggcg cagcccgcgc ttgtccagcc ccatgcgccg
     4801 cgactcgccg ccggcgagca gtaccccgac gcgtaccgtg tcctggtctg tcggcggagg
     4861 ggccatggcg aggatggagt gtagctcatg tggagcggcg cggcggaagc ggcaagcgcg
     4921 cttgacaccg ggtcctgcga cgatacgatg cccgtgatgc gggcgagcat gtccgcatcg
     4981 cggatgaatg tggcgttcgg gggaacgcgg tcggccgcgg tcggccgccg gtgtcgcatc
     5041 tgggaagcga ggtcttacga gtatgggtga agtccagcga ctcacaaact gcacgaccgg
     5101 cggtccggtc ttcgtggacg ttgaggacgg caggatcgtc cgcatgtacc cgatcgacct
     5161 cgccgacgac gacaagggcg actgggtgat cgaggcgcgg ggcaggaagt tcacgccgcc
     5221 gcgccgcacg acgctctctc cgcacgcgca ggcgcagcgc tccatggtct actcgccgac
     5281 gcgcatcctg catccgatga agcgcgtcga ctgggacccc gagggcgagc gcaacaccca
     5341 caaccgcggc gtctccggct acgaggagat cagctgggac gaggcgctcg acctggtgac
     5401 caccgagctg atccgcgaga agcgcgagaa cggcccgggc tccatcctca ccacctgcag
     5461 ctcgcaccat ctgtggggca acctcggcta ccgtcacagc gcctactacc gcttcctcaa
     5521 cctcatgggc atggtgcact ctgcgcacaa ccccgacagc tgggaaggct ggcactgggg
     5581 ggccatgccg atgtggggca acagccaccg gctcggcatc cccgagcagt acgacctcct
     5641 cgaggacgcg ctcaagcaca ccgagatgat cgtcttctgg tcgtccgacc ccgagagcac
     5701 cggcggcggc atctactccg ccttcgagag cacctcacgc cgctactgga tgaaggagct
     5761 cgggatcaag atggtcttcg tcgatcccta ctacaacccg accgccgagc tcttctccga
     5821 caagtggttc gcgccgcgcc tcggcaccga cgtcgccttc ggcctcggca tcgcccacgt
     5881 gtggctcgcc gagggcctct acgacaagga gtacgtcgcc gcgcgcacca ccggcttcga
     5941 cgagtggcgg gactacgtgc tcggcaagac ggacaacgtc cccaagacgc ccgagtgggc
     6001 cgagagcgag tgcggcatcc ccgcccgcca gatccgcgcc ctcgcgcgcg agtggggaag
     6061 caagaaggtc atgctcgccg ccggcggcct gggcggctgg ggcggcgcct gccgctcggc
     6121 cacgggcaac gagtgggcgc gcaccatggt ggcgctcgcg gcgatgcagg gctacggcaa
     6181 gccgggcgtc aacatctggg gcacgtccca gggcgcgccg tcggactgtt ccttcgtgtt
     6241 tcccggctac gccgagggcg gcatgtccgg cgacaccgag aagtcggccg ccggcttccg
     6301 catgctctac cgcatcttca agcccgagac cgggctgcac ccgacggtga acgcgcacga
     6361 ctcacccgag ggcatcgcca tcccgcgcct gcgcatcccc gaggcgatga tgcacgagaa
     6421 gttcgagtgg cgcggcaagg gcttctgcgg cccgtcgatc cagagccaga tgcagaagta
     6481 ccagtacccg gcgccgggct acccgttcgt caccatgtac tggcgctacg gcggctcctt
     6541 cttcggcacg atgcagcaga ccaaccgcta cgtgaaggcg taccgcgagg gcggcgtgcg
     6601 caccgtcgtc aaccagtcca tctggatgga gggcgaggcg aagttcgccg acatcatcct
     6661 cccggcgtgc accaacttcg agcgctggga catgagcgag ttcggcaact gctccggcta
     6721 catcccggac aaccacaccc agaccagcca ccgcgtgatc gtcttccaga agaagtgcat
     6781 cgagccgctc ggcgagagca ggtcggacta cgacatcttc gccgccgtcg ccgggcgcat
     6841 gggcatgggc gacgtcttca ccgagggcgg catggacgag tacgactggt gcaaggcgta
     6901 cttcgacgcc accgacctgc cgcagatcac gacctgggag gagttcgagc agaaggggta
     6961 cgtcgtcgtg ccgatcccgg acgactacaa gagcacgccg gcgatgcgct ggttcgccga
     7021 ggggcgcaag cgcgacactc ccgactgggg gccgaggccc ggcgacaccg tcggcttcga
     7081 gggcctgcag acgccgtccg gcaagatcga gttcgtgagc cgcacgctcc agcacctcga
     7141 actggacgtc gtcgatcccg agcggccggc gatgggaccg cagttcatcc cgagctggga
     7201 gggccatcac acgacggagc tgtacggcac ctacccgctg cagatggtga gcccgcaccc
     7261 gcgcttcagc ttccacacca tgggcgactc caagggcacg tggctgaacg aggtggagga
     7321 gcaccgcatc ctgcacgagg acggccaccg ctactggatc atgcgcctga acagcgtgga
     7381 cgcggcggcg cggggcatcg ccgagggcga cctgatccgc gcctacaacg accgcggctc
     7441 ggtcatcctc tccgcgcaga tcaccgagcg cgtggcgccg ggcaccgtgc actcctacga
     7501 gagctgcgcc gactacctgc cgaccggcga gcccggcgag tccaccgaca tcgccggctg
     7561 cgtcaacatc ctcaccaaca aacggttcat caccccgacg tccatcgcga tgtccaacaa
     7621 ctcctgcctg atccaggtcg agaagtggga gggggaggct tcatgaagag ctggcacctg
     7681 atcgtcgacg tcgcccgctg ccacgactgc aacgactgct tcctggccga caaggacgag
     7741 ttcgtcgaca acgagttccc gccgtactcg gcggcccagc cctggcacgg ccaccgctgg
     7801 atgaacatca agcgcaagga gcgcggccag tacccgctgg tcgagaccgc ctacctgccg
     7861 atgccgtgca tgcactgcga cgaggcgccc tgcctcaccg ccgacggcgc cgtctacaag
     7921 cgcccggacg gcctcgtcat catcgatccg gtcaaagcca gggggcgcaa ggagatcgtc
     7981 gccagctgcc cgtactcggc catctactgg aacgaggaga agcagctggc gcagaagtgc
     8041 accggctgcg cccacctcct cgacgacggc tggaccgaga cgcgctgcag ccaggtgtgc
     8101 cccaccgagg cgatcaagct cgtgctcgcc gaagaggacg acgtggagcg gatggtcgag
     8161 gaggagggcc tcgcgcgcta ccgcgactct ctgggcacca ggccgcgcgt gtactacaag
     8221 aacctccacc gctgggacaa ggcgttcgtc ggcgtgagcg tggtcttcgg cgacagcgac
     8281 gagtgcgccg agggcgcgcg cgtctcggtg tcgcgtgagg gcgcggcgac gcgcgagggc
     8341 acgaccgaca tcttcggcga ggtcaagatc gaccacctca cgccgggccg cgagtacgcc
     8401 gtgaagatcg aggccgacgg ctacaaggcc gcggagctca cggtccagtt ggacgccagc
     8461 gtcaacgccg ggacggtggt cctggagaag gcgtgaggag agggccggcg cccctcgaag
     8521 cggcggcgcc ggcctcaagt tcgctgcggc cccccggagc cggacccccc cgcccggcct
     8581 ccatccccgc agcagcagcc cgcgacgttc cacggtcgcg aggcgcgtga ggggcgccgt
     8641 cccgtgtgtc ccgggacggc gccccgtctc gccggccgtc gcgccgccaa gttgaccggc
     8701 ctccgatgac ggtgcgagca tggcgagcga tttcgtcggc gcgggagaaa ggcgtgcacg
     8761 acatgg
//
LOCUS       DASUMX010000226        13389 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_81926, whole genome shotgun sequence.
ACCESSION   DASUMX010000226 DASUMX010000000
VERSION     DASUMX010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 13389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13389
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81926"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            282..1781
                     /locus_tag="VFZ86_10680"
     CDS             282..1781
                     /locus_tag="VFZ86_10680"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEX5642783.1"
                     /translation="MTGTTVGERAHVQTRGFPRLVGRITALSRRRVLVTTLVVVTILG
                     ALALDLAIPSYPIAGFYLVPVTLAALTLRVRTTIAVSLLCLGLALYVIVIQDRWDGPT
                     ITVVCFSVLSGAALIALAYLFKQVDQLYETERSTTEMLESLAAQLQTLQEVVVLDSDR
                     PLSELLRRVIDQASQLLGSDGCCVYRYDPQRDVLEVAAASGLSSCGGTLPATGSAEPV
                     ARALAGHAPVAVSRSREGGALLAVPLLVRSEAYGVLALTYLEERAFTDLDIRLAASFG
                     GQVALAIENARLRDEIGQAAAATERSRLARDLHDSVTQSLFAASLKAEAVRRRWRPSS
                     EEARDNVQDVERLARGALAEMRTLLLEMRPDALAEASLATLIEQLAAATGGGSRVTVH
                     VDLRGSAPLPQDVSVALFRIAQEALQNVNRHSGATEAWVMLDMTGPGVRLSVRDDGHG
                     FDEAAVTPEHLGLAMMRERGEESHVEVTVESAPGAGTTVTAVWRPDEEP"
     gene            1778..2443
                     /locus_tag="VFZ86_10685"
     CDS             1778..2443
                     /locus_tag="VFZ86_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012188659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5642784.1"
                     /translation="MSEGGGVPTGAGEQRIKVVIVDDHVLVRSGLEVVLGMFDDIELV
                     GQAGDGEEAVRLCREIRPDVVLMDLVMPGVSGVEATQQILATSPGTKVVALTSFTEDA
                     LIGETLRAGAIGYLMKNVTADQLADAVRAAHAGRSTLAPEAADALVRSVSAPPPGADS
                     LTAREREVLKLMADGLTNADIAERLVIGVATVKTHVSSIISKLAVSTRTEATATAIRR
                     GLV"
     gene            2751..5105
                     /locus_tag="VFZ86_10690"
     CDS             2751..5105
                     /locus_tag="VFZ86_10690"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5642785.1"
                     /translation="MARRKRREGHSLHKVLGVGALFSTAYGNVGSSIYYALGVVAVAA
                     LGLTPAVFVITGALFVCTAWSYSEATAAMPEAGGASSFTRRAFNEFASFGIGWAQMLT
                     YTATIAISALFVPQYLSIFWPILKEPPYNVIGGAITILILVVLNVVGIKEAASLNVVL
                     ALLDLGTQVLIMIIALVLLLEPNILISQIHWGIAPTWSQFLYGLAMGTVAYTGIETIS
                     NMAEEAHHPDKDVPRSMNFVIIAVLVVYIGMPLAALSSMTVDYNTLAVDPTTGMTVPV
                     EVVPGEPEGTYRLKSDPTVTAYVPLEERADGTMVIPAQEPTGEVTTVDGQQVTRLYGT
                     QLGSNFLADPVLGMVRFLPDNVAWLRWILGPWVGILAATILFIATNAGIIGVSRLAFS
                     LGQHRQLPRVLGRVHPTRLTPYIAIVLFGVVAVILILPGEIPLLADLYAFGSMIAFTA
                     AHVSVIVLRRKEPDLARPFRPPLNITIRGTSYPLTAVLGAMGTFSVWCVILYYKPWSG
                     LIGIAWVGIGIVAYVIFRKVHGYSLTRTVKSPTLSASLQEDVVYDQLLVPVRDTQVSE
                     EMMVLACQLATERRSSIDALYVIEVPLNLPIDASLPEERERARQVLENAAQAADMFGV
                     KLTPVMVTARSAGRAIVEEAIARRSEVIVLGSQGKRRIADKVFGRTIDYVLNNLPCEA
                     IINVIPKTTPGVAGVTAAASAGAVSSSAPPGAGPSGEVPAAAGGEAATGRGRARRRAH
                     GLPLSRYLPSRSGRDGSGAGDDAGSAAEGPVGAAPPDAGKRGSE"
     gene            complement(5118..6518)
                     /locus_tag="VFZ86_10695"
     CDS             complement(5118..6518)
                     /locus_tag="VFZ86_10695"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX5642786.1"
                     /translation="MRTRSLRWRLALTYAGMALLTAVILGGILIAVLANHYTRAEDSY
                     LDASAKLVVADPPPLTTYQDLTWWAQAAALTTQTRVQVYAQDGTLVVDSGSPDELDAE
                     ALLDRDDGFGHGGRERDRDGLPNPLGNGLFGTGGGARSDRSLTMDMGNGAYVKLSEGP
                     ASGRDALMSAAVAWILAAALAVGLAALAGSLIAARIARPVVALTEASDRMAAGDLGAR
                     APVAGGDEVGRLGESFNGMAARVETTVTSLRRFVADAAHEIGTPLTALQADLELAERK
                     ATSADERRLVDRALAQTGRLASLSDNLLQLSRLEAGEPAGERQSTDLAAVALELADGV
                     ASRAEQAGVELVLDVAAGPLPVPMGHARLQTVLANLLDNAVKFTPEGGSVTLATRREG
                     AQVVATVADTGVGIPADEQREIFERFHRARNVSAYPGNGLGLAIVKAAVERSGGTVSF
                     ASSEAGTTFVVRLPLA"
     gene            complement(6530..7231)
                     /locus_tag="VFZ86_10700"
     CDS             complement(6530..7231)
                     /locus_tag="VFZ86_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005281774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5642787.1"
                     /translation="MAEHAQTILVIEDDPAVGEALVEGIGTEGYRVHWDTTGGEGIAF
                     ARDHSPQLIVLDVRLPDGNGFDFCRQIRQLGLRQPILMLTVQSDQMDKVLGLEMGADD
                     YLTKPYHLRELIARIRSLLRRAYGEFASTEADVLYAGDLTIDRTRATVTRDGRSLNLT
                     PTEFRLLVFLAQHPGQVFSRGQLIENVWGSDADFYDDKTVSVHVRRLREKIEHEPSNP
                     SVILTVPGIGYRLAI"
     gene            complement(7307..7873)
                     /locus_tag="VFZ86_10705"
     CDS             complement(7307..7873)
                     /locus_tag="VFZ86_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642788.1"
                     /translation="MSDPTKENASATPPENPPAGEAPEAATPATPAAEAAPAQDQVAA
                     AAAEQPTAAMPPAYPAAPAGAPAATGGPWYRRRWAVVTGAVAAAVILFLGGMAVGTTI
                     DGDDRGEFRGDAGPGMFRDGSGGGFGHDGWGDTGRGGGQGMVPPGMGQGGQGHGWDRD
                     GDGDPHGWQTPGATPTPDPSTTPQALQL"
     gene            8105..8611
                     /locus_tag="VFZ86_10710"
     CDS             8105..8611
                     /locus_tag="VFZ86_10710"
                     /inference="COORDINATES: protein motif:HMM:NF025716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4405 domain-containing protein"
                     /protein_id="HEX5642789.1"
                     /translation="MTVRARLWLDLALFGALFLAYNPAWTGVAVHEWLCVIAIAPLLF
                     HVIVNWDQTLQILRRFAELVKTTPKVNLVVDAALFVAAVTVMLSGLLISQSVARTLGI
                     TIVPDALWMSTHAWSADATILLLLVHFVLHWRWIVNVAGKLGRPRRQAPADPLAAIRL
                     ATRRLDAP"
     gene            8632..8961
                     /locus_tag="VFZ86_10715"
     CDS             8632..8961
                     /locus_tag="VFZ86_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642790.1"
                     /translation="MKNLSRNTAAVLVITLFTGLAVFSAIQLGGRLFGTSTASAQTLT
                     SQAVTGQLEDSSDGVESVDEYYYDDGSGGYGNGYGGSSSADSGSASTTQTCPATGCSA
                     TSCHATQ"
     gene            complement(9077..10567)
                     /locus_tag="VFZ86_10720"
     CDS             complement(9077..10567)
                     /locus_tag="VFZ86_10720"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX5642791.1"
                     /translation="MDDSDGSDGDGEGVGVQALAHRDTQLEALAGGRPGAFEQFYRDH
                     HARIYNLAARIVGDPDDAADITQEVFLRAYAHPLQAGDGARPEPWLYRIAVNASYDHL
                     RRRAARPSTSLEAVPEVAARRDFFAAAETTRLVEGCLAGLTPRYRTALILKDLHGLST
                     AEIAQVMDLHEGTARVVLHRARSAFRKAFRSAAPTGAGGASALGLAAFLPDLPIPAQL
                     HSPPVIDFAPPVAGPPAAPHAARPPAGAEQALLPGGAPVAAPVGVPAAATAAAACTPA
                     PAGILAGAGGIAGFKVAVAVLATVVVTGGGVAAGHFADSPAVRDVAGTAVRTVAATSP
                     LAGHNGATWTHREMIRQRLQAAGLAPSGAGSGSAAGGAAVRDGSGGGAQSQVGPVAGS
                     SNGAEASTGGGAVTGGAATGSGVASQATGGGSGSGATAGGSEGGAQGTDTGEAGTSTG
                     GTDAGGDAGGTGGGETDSGGGSGDTPPPDGTGTGTGSGTGTGGGGT"
     gene            complement(10664..11221)
                     /locus_tag="VFZ86_10725"
     CDS             complement(10664..11221)
                     /locus_tag="VFZ86_10725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642792.1"
                     /translation="MRLVKFLVLIVVVGLAASSIALARDQLQDKDQIQQQDKIQLNDC
                     DVSCDQTPDQDRTRDQLKTQDCLLVSATADESGDLVKARDRDRDRDGSCDGSAAQDRT
                     RTRVRTQSESCDETCAQTQAQTQTQTQTQAQTQTQAQTQTQTQAGTQTQAGTQTQTQT
                     GQPDDAGSGGGSTSGSGSGSSGGGH"
     gene            11608..13353
                     /locus_tag="VFZ86_10730"
     CDS             11608..13353
                     /locus_tag="VFZ86_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642793.1"
                     /translation="MKKLALILIAAAALAVAFGAAASTTAAETVTYQDYTLPGVEFDW
                     QTVVNNGVQAPGDPRKFNSYNPPSLNVDRLVVFRARTKGGTGGQPAHGVFTRDMGVAA
                     APVTTIFDRKTAVPQPNNIVWQPTDLLTTFIEPPSFPRIDMRSDTVASRGNHQPVWGY
                     VLPDGSETRAGHTGIYTDPYGTLITGASNLGAIPDFPFFAVPGTDGVLFDVFPGAPAV
                     TDGATVVFKGNYTLGTLGRTGVYYRDLTDSPIPLLDGVTQLAPAGGTGPVVRIADTDT
                     IIPGTRTTRFGSTAPPSAARRLAVFAGFDNEASPTKGGIYRAPLTGADPTLTTLVKIG
                     GQVPGEKKGVVFNRLGEGVSFDGRFVAFWGAWGSATRKLVLQCPEEGNAARLAYCREL
                     YPNGFATTVPVNQGIFVYDTVGRTTAVVAKSPSNFSDFVYWNFSGRVPGVGEGGEDDD
                     TGEPARWRSATFVAVSGLVDGSLTDATFHVAFKARTGRIVDGAYVDPVDGIYLRKGPN
                     KAPIAAVVETGMDGTLLDPAAVVPGTQVPIPVTSMGIERDGFRGDALAINVSMGSEEA
                     GWAGIYLATVPAALR"
BASE COUNT         1797 a         4770 c         4825 g         1997 t
ORIGIN      
        1 ctccaggtac gtgcggcagc tgtagcggcg gtcgatgagc tcgcgggcgc ccgtcacctc
       61 tgcacctcct cctggattcg aagcatcacc gctcccttgc cttcggcacg cgcatggatg
      121 gccctccagg gaatcgagtc cagtcgcgcg tctgcactcc ggcaatgcta ccgttgtcgt
      181 ccgaaggggt cgacgggagg atggacctgg tgttccgggc gagggccgtt gttggccgcc
      241 gccggcggcg gtaggctggg cgccggcgac ggaggagcgg tgtgaccggt acgacggtcg
      301 gtgagcgggc ccatgtgcag acccgggggt tcccgcgact cgtcgggcgg atcaccgccc
      361 tgagtcgccg tcgcgtgctg gtgacgacgc tggtggtggt caccatcctc ggcgctctgg
      421 ccctcgacct ggcgatcccg agctatccca tcgccggctt ctacctcgtc ccggtgacgc
      481 tcgcggcgct gaccctgcgc gtgcgcacca ccatcgccgt gagcctgctc tgtctgggcc
      541 ttgcgctcta cgtcatcgtg atccaggacc gctgggacgg tccgaccatc accgtcgtgt
      601 gcttcagcgt cctcagcgga gccgcgctga tcgccttggc ctatctgttc aagcaggtcg
      661 accagctgta cgagacggag cgctccacca ccgagatgct cgagtcactc gccgcccagc
      721 tgcagaccct gcaggaggtc gtggtcctcg actcggaccg tcccctgtcc gagctcctgc
      781 gccgcgtgat cgaccaggcc agccagcttc ttgggagcga cggctgctgt gtctaccgct
      841 acgacccgca gcgcgacgtc ctcgaggtgg ccgccgccag tggtctgtcg tcgtgcggcg
      901 gcacgttgcc ggccacgggg agcgccgagc ccgtcgcccg cgcgctcgcc ggccacgccc
      961 cggtcgcggt ctcccgttcg cgcgagggcg gcgcgctgct cgccgtgccc ctcctcgtgc
     1021 gcagcgaggc ctacggcgtg ctcgcgctca cctacctcga ggagcgcgcc ttcaccgacc
     1081 tcgacatccg cctcgccgcg tccttcggcg ggcaggtcgc gctggccatc gagaacgcgc
     1141 gtctgcgcga cgagatcggg caggcggccg cggccaccga gcgctcgcgc ctggcccgcg
     1201 acctgcacga ctccgtgacg cagtcgctgt tcgccgcgag cctcaaggcc gaggccgtcc
     1261 gccgtcgctg gcggccgagc agcgaggagg cgcgcgacaa cgtgcaagac gtggaacggc
     1321 tcgcgcgcgg ggcgctcgcc gagatgcgca ccctgcttct cgagatgcgt ccggacgcgc
     1381 tcgccgaggc gtcgctcgcc accctcatcg aacagctcgc ggcggccacc ggaggcggct
     1441 cgcgcgtgac cgtgcacgtg gacctgcgcg ggagcgcgcc gctgccccag gacgtcagcg
     1501 tggcgctgtt ccgcatcgcc caggaagcgc tgcagaacgt gaaccgccat tccggcgcga
     1561 ccgaggcgtg ggttatgctc gacatgacgg gacccggcgt acgcctgtcc gtgcgcgacg
     1621 acgggcacgg gttcgacgag gcggcggtga cgccggagca cctcgggctc gcgatgatgc
     1681 gtgaacgcgg ggaggaatcg cacgtcgagg tcaccgtcga gagcgcgccg ggcgccggca
     1741 ccacggtgac cgcggtctgg cgtccggacg aggaaccatg agcgagggag gcggcgtgcc
     1801 cacaggcgca ggcgagcaga ggatcaaggt cgtcatcgtc gacgaccacg tgctggtgcg
     1861 cagcggcctc gaggtcgtgt tgggcatgtt cgacgacatc gagctggtcg gccaggcagg
     1921 cgacggcgag gaggccgtgc ggctgtgccg ggagatccgt cccgacgtcg tcctcatgga
     1981 cctcgtcatg ccgggcgtgt ccggggtgga ggcgacgcag cagatcctcg ccacgtcgcc
     2041 cggcaccaag gtggtcgccc tgacgagctt caccgaggat gccctcatcg gcgagacgct
     2101 gcgcgccggc gccatcggtt acctcatgaa gaacgtgact gccgaccagc tcgccgacgc
     2161 cgtgcgcgcg gcacacgccg ggcgctcgac gctggctccg gaggcggccg acgcgctcgt
     2221 gcgctcggtc tccgcgccgc ccccgggcgc cgattccctc accgcccggg agcgtgaggt
     2281 gctcaagctc atggcggacg ggctcaccaa cgccgacatc gccgagcgcc tcgtcatcgg
     2341 cgtcgccacc gtcaagacgc acgtcagcag catcatctcc aagctcgcgg tgtccacgcg
     2401 caccgaggcc accgcgacgg ccatccgccg cgggctcgtc tgactcccgc cgcggctcac
     2461 gcacctcatc cgttggtccg cctcatcctt aaggcggacg cagggctcct ctgaaaaggt
     2521 gggccgagtc tcccccgcaa gctcgatgta gggcggggcc gggactgctt caatggagtc
     2581 tcgccagcag tcccggtccg tcgcgtagga ggatgaggtg atcgagagga tcctgcttcc
     2641 cgcgctcacg ggcagcgtct cgcgggcagt cgtcgtgtgc gccggctgcc cggtcatggt
     2701 cgtgcgcgag cccggctcca gggtctgacc gggctcgcac aggacccgcc gtggcacgcc
     2761 gcaaacgccg agaaggacac tcgctgcaca aggtgctggg ggtcggagcc ctcttcagca
     2821 ccgcgtacgg caacgtaggc tcgtccatct actacgccct cggcgtcgtc gctgtggccg
     2881 cgctgggctt gacgccggcg gtgttcgtca tcaccggcgc gctcttcgtg tgcacggcat
     2941 ggtcctactc cgaggccacg gccgccatgc ccgaggccgg cggcgcctcc agcttcacgc
     3001 gccgcgcctt caacgagttc gccagcttcg gcatcggctg ggcgcagatg ctcacgtaca
     3061 cggccaccat cgccatctcg gccctgttcg tgccgcagta cctctcgatc ttctggccga
     3121 tcctcaagga gccgccgtac aacgtcatcg gcggcgccat cacgatcctc atcctcgtcg
     3181 tcctcaacgt cgtcggcatc aaggaggcgg ccagcctcaa cgtggtgctc gcgctcctgg
     3241 acctggggac gcaggtcctg atcatgatca tcgcgctcgt cctgctgctg gagcccaaca
     3301 tcctcatttc ccagatccac tggggcatcg cgccgacctg gagccagttc ctctacggcc
     3361 tcgccatggg caccgtggcg tacacgggca tcgagaccat ctccaacatg gccgaggagg
     3421 cgcaccaccc cgacaaggac gtgccgcgct ccatgaattt cgtcatcatc gccgtcctgg
     3481 tcgtctacat cggcatgccg ctggcggcgc tctcgtcgat gaccgtcgac tacaacacgc
     3541 ttgcggtcga ccccacgacc ggcatgaccg tgccggtcga ggtcgtcccc ggggagcccg
     3601 agggcaccta ccgcctgaag tccgacccga ccgtgaccgc ctacgtaccg ctcgaggagc
     3661 gggcggacgg gaccatggtc atcccggccc aggagcccac cggcgaggtc accacggtcg
     3721 acgggcaaca ggtgacgagg ctgtacggca cccagctcgg cagcaacttc ctcgccgacc
     3781 cggtgctggg catggtgcgc ttcctgccgg acaacgtcgc ctggttgcgc tggatcctcg
     3841 gcccttgggt cggcatcctg gctgccacca tcctgttcat cgccaccaac gccggcatca
     3901 tcggcgtctc gcgcctggcc ttctcgctgg gccagcaccg ccagttgccg cgtgtgctcg
     3961 ggcgcgtcca tcccacgcga ctcaccccct acatcgccat cgtgctcttc ggcgtcgtcg
     4021 cggtgatcct catcttgccc ggcgagatcc cgctgctggc ggacctctac gccttcggct
     4081 cgatgatcgc gttcaccgcc gcgcacgtgt cggtgatcgt gctgcggcgc aaggagcccg
     4141 acctggcgcg gccgttccgg ccgccgctca acatcaccat ccggggcacg tcgtacccgc
     4201 tcaccgccgt gctgggcgcg atgggcacct tctcggtctg gtgcgtgatc ctgtactaca
     4261 agccgtggag cggcctcatc ggcatcgcct gggtgggcat cggcatcgtc gcctacgtga
     4321 tcttccgcaa ggtgcacggc tactcgctca ccaggacggt caagtcgccc acgctgtcgg
     4381 cgtcgctcca ggaggacgtg gtctacgacc agctgctggt gccggtgcgc gacacccagg
     4441 tgagcgagga gatgatggtg ctggcgtgcc agctggccac cgagcgccgg tcgtcgatcg
     4501 acgcactcta cgtgatcgag gtgccgctca acctgccgat cgacgcgagc ctgccggagg
     4561 agcgcgagcg ggcgcggcag gtgctcgaga acgccgccca ggccgccgac atgttcggcg
     4621 tgaagctcac gccggtcatg gtcaccgcac ggtcggcggg gcgggcgatc gtggaagaag
     4681 ccatcgcccg ccgctccgag gtcatcgtcc tgggctcgca gggcaagcgg cgcatcgccg
     4741 acaaggtctt cgggcgcacc atcgactacg tgctcaacaa cctgccctgc gaggcgatca
     4801 tcaacgtgat ccccaagacc acgccggggg tggcgggggt gaccgcggcg gcgtccgccg
     4861 gcgcggtctc atcgtccgcg cctccgggcg ccggcccctc gggcgaagtg ccggcagcgg
     4921 ccggcggcga ggcggcgacg ggtcgcggca gagccaggcg cagggcccat gggctgccgc
     4981 tgagccggta cctgccgtcg cggtccggac gggacggatc cggagccggg gatgatgccg
     5041 gcagcgctgc ggagggcccg gtcggggcgg ctccgccgga cgccggcaag cgcggctccg
     5101 agtaggcgag gcgcgctcta cgcgagcggc aggcggacca cgaaggtggt gccggcctcc
     5161 gagctcgcga acgagacggt gccgccgcta cgctccaccg ccgccttgac gatggcgagc
     5221 cccaggccgt tgcccgggta ggccgagacg ttgcgcgcgc ggtggaagcg ctcgaagatc
     5281 tcgcgctgct cgtccgcggg gatgccgacg ccggtgtcgg ccaccgtggc caccacctgc
     5341 gcgccctccc tgcgcgtcgc gagggtgacg ctgccgccct ccggcgtgaa cttgacggcg
     5401 ttgtcgagca ggttggcgag gacggtctgc agccgggcgt gccccatcgg gacggggagg
     5461 gggccggcgg cgacgtcgag caccagctcg acgccggcct gctccgcccg tgaggccacg
     5521 ccgtcggcaa gctccagcgc gacggcggcg aggtcggtgc tctggcgctc gccggcgggc
     5581 tcgcccgcct cgaggcggga gagctgcagc aggttgtccg agaggctggc gaggcggccg
     5641 gtctgggcga gcgcccggtc gacgaggcgg cgctcgtcgg cgctcgtcgc cttgcgctcg
     5701 gccagttcga ggtcggcctg cagggcggtg agcggcgtgc cgatctcgtg cgccgcgtcg
     5761 gcgacgaacc ggcgcagcga ggtcaccgtg gtctcgacgc gggcggccat gccgttgaag
     5821 gactcgccga ggcgcccgac ctcgtcgccg ccggcgacgg gggcgcgggc gccgaggtcg
     5881 ccggccgcca tgcggtcgct cgcctccgtg agcgcgacca ccggtcgcgc gatgcgcgcg
     5941 gcgatcaggg agccggcgag ggccgcgagg ccgacggcga gcgcggccgc gaggatccag
     6001 gccacggccg cgctcatcag cgcgtcgcgg ccggacgccg gcccctcgga gagcttcacg
     6061 taggcgccgt tgcccatgtc catggtgagc gagcggtcgg agcgcgcgcc gccgccggtg
     6121 ccgaagagcc cgttgccgag ggggttcggc aggccgtcgc ggtcgcgctc gcggccgccg
     6181 tggccgaagc cgtcgtcgcg gtcgagcagc gcctccgcgt caagctcgtc tggcgagccc
     6241 gagtcgacga cgagcgtgcc gtcctgcgcg tacacctgca cgcgcgtctg ggtggtgagc
     6301 gccgccgcct gggcccacca ggtgaggtcc tggtacgtgg tgagcggcgg cgggtccgcc
     6361 accaccagct tggcgctcgc gtccaggtac gagtcctcgg cgcgcgtgta gtggttggcc
     6421 agcacggcga tgaggatgcc gccgaggatg acggccgtca gcagcgccat gcccgcgtag
     6481 gtgagcgcga gccgccatct gagcgagcgg gtccgcacgg ccccgcgcct cagatcgcca
     6541 gccggtaccc gatgccgggc acggtgagga tcacggaggg gttgctcggc tcgtgctcga
     6601 tcttctcgcg tagcctgcgc acgtggacgc tcacggtctt gtcgtcgtag aagtcggcgt
     6661 cgctgcccca cacgttctcg atgagctggc cgcggctgaa gacctggccc ggatgctggg
     6721 cgaggaacac gaggagccgg aactcggtcg gcgtgaggtt gagcgagcgg ccgtcgcggg
     6781 tgacggtggc gcgggtgcgg tcgatggtca ggtcgccggc gtagagcacg tccgcctcgg
     6841 tggacgcgaa ctcgccgtag gcacggcgca ggagcgagcg gatgcgggcg atgagctcgc
     6901 gcaggtggta gggcttggtg aggtagtcgt cggcgcccat ctcgaggccg aggaccttgt
     6961 ccatctggtc cgactgcacg gtgagcatga ggatgggctg gcgcaggccg agctggcgga
     7021 tctgccggca gaaatcgaag ccgttgccgt cgggcaggcg tacgtcgagg acgatgagct
     7081 gcggagagtg gtcgcgagcg aaggcgatgc cctcgccccc ggtggtgtcc cagtggactc
     7141 tgtagccctc ggtcccgatg ccctcgacga gggcctcgcc caccgcgggg tcgtcctcga
     7201 tgacgaggat ggtctgggcg tgttcagcca tggcgacagc gtagcacggg catgcggccg
     7261 cccccgccgg gcgaggcagg ggcggccgca cgggtccgcg ctcgggtcac agttggagag
     7321 cctgcggcgt ggtggaaggg tccggggtgg gcgtcgcgcc cggggtctgc cagccgtgag
     7381 ggtcgccgtc gccgtcgcgg tcccagccgt ggccctggcc gccctggccc atgccgggag
     7441 ggaccatgcc ctggccgccg ccgcgaccgg tgtcgcccca gccgtcgtgg ccgaagcccc
     7501 cgccgctgcc gtcgcggaac atcccgggac cggcgtcgcc gcggaactcg ccgcgatcgt
     7561 cgccgtcgat ggtggtgccg acggccatgc cgccgaggaa gaggatcacc gcggcggcca
     7621 ccgcgccggt gacgaccgcc cagcggcgcc ggtaccaggg gccgcccgtg gcggcgggag
     7681 cgccggcggg cgcggccggg tacgccggcg gcatggccgc cgtgggctgc tcggccgcag
     7741 cagccgcgac ctggtcctgc gccggcgccg cttccgctgc gggcgtggcc ggggtcgccg
     7801 cctccggcgc ctcgccggcc ggcgggttct ccggcggcgt cgcgctggcg ttctccttgg
     7861 tcgggtcgga catgacgcac ctctctgtcg ggtcgcgtgc cggcacagcg ccggtgtggt
     7921 gggacgacta tctccggccc aggttgcgcg ctgtctgcgc ggctactaca ggtccgtaac
     7981 cccggcggag agacggttac ggacctgtag ccgccgctca atcccggctt catatccgtc
     8041 tcctacagtg gtcgtggccg tcatgaggcg gcccggagcg gaccccccga cgaaaggagc
     8101 gcccatgacc gtgcgagccc gcctctggct cgacctcgcg ctgttcggcg cgctcttcct
     8161 cgcctacaac cccgcgtgga cgggcgtggc ggtgcacgag tggctgtgcg tgatcgccat
     8221 cgcgccgctg ctcttccacg tgatcgtgaa ctgggaccag acgctgcaga tcctgcggcg
     8281 cttcgccgag ctcgtgaaga cgacgcccaa ggtcaacctg gtggtggacg ccgccctctt
     8341 cgtcgccgcg gtgacggtga tgctgagcgg gctgctgatc tcgcagtcgg tcgcaaggac
     8401 cctcgggatc acgatcgtgc cggacgcgct gtggatgagc acgcacgcgt ggtctgccga
     8461 cgcgaccatc ctcctgctcc tcgtgcactt cgtcctgcac tggcgctgga tcgtgaacgt
     8521 ggccggcaag ctcggccggc cgcgtcggca ggcgccggcc gaccccctgg ccgcgatccg
     8581 cctggcgacg cgccggctcg acgccccctg ataccccatc gaaggagaac gatgaagaac
     8641 ctgtccagga acaccgcagc ggtcctcgtc atcacgctgt tcaccggtct cgccgtgttc
     8701 tcggcgatcc agctcggcgg tcgtctgttc ggcacctcca cggcgagcgc ccagacactg
     8761 accagccaag ccgtgaccgg ccaactagag gactcgagtg acggcgtgga gagcgtcgac
     8821 gagtactact acgacgacgg gtcaggcggc tacggcaacg gctacggcgg gtcgagcagc
     8881 gcggactcgg gcagcgcctc gaccacccag acgtgtccgg cgacggggtg cagcgcgacg
     8941 agttgccacg cgacgcagtg acgcccggct gacgaacgcg cgtaggcggg gaggacgggt
     9001 acgtcagacg agcgtggcga ctgagccggc ccggtcgtga agacaaggtt ccggccggtc
     9061 ccgtagccgg ttcggctcag gtgccgcccc cacccgtgcc ggtcccgctg ccggtcccgg
     9121 tgccggtccc gtccggggga ggggtgtcgc ccgagccgcc gccggagtcg gtctctcctc
     9181 cgccggtccc gccggcgtca ccgccggcgt ccgtccctcc ggtgctcgtg cctgcctcgc
     9241 cggtgtcggt cccttgggcg ccgccctcac tgcccccagc ggtggcgccc gacccggagc
     9301 ccccaccggt cgcctgcgag gcgacgccgc tccctgtggc ggcgcccccc gtgacggcgc
     9361 cgccgccggt acttgcctcg gcgccgttgc tgctcccggc gaccgggccg acctgggact
     9421 gcgcgcctcc gccggagccg tcgcggacgg ccgccccgcc ggcggcggac ccggagccgg
     9481 cgccactggg cgccagcccg gcagcctgca ggcgctggcg gatcatctcc cggtgcgtcc
     9541 aggtcgcgcc gttgtgccct gcgagcgggg acgtcgcagc gaccgtcctg acggcggtcc
     9601 ccgcgacgtc gcggacggcg ggtgagtccg caaagtgccc ggccgcgacc cccccgccgg
     9661 tgacgaccac cgtcgcgagc accgcgaccg cgaccttgaa gccggcgatc ccgccggcgc
     9721 cggcgaggat cccggccggc gccggcgtgc aagcggccgc cgccgtcgct gccgcgggca
     9781 cgcccaccgg tgcagcgact ggtgcgccgc cgggaagcag cgcctgctcg gcccctgccg
     9841 ggggacgggc agcgtggggc gcggcgggcg gccccgctac cggcggcgcg aagtctatga
     9901 ccggcggcga gtgcagttgc gccgggatgg gcagatcagg caggaaggcg gcgaggccga
     9961 gcgccgacgc gcccccggct cccgtgggcg cggccgaacg gaaggccttg cggaacgccg
    10021 agcgcgcccg gtgcaggacc acgcgcgccg tgccctcatg caggtccatc acctgcgcga
    10081 tctcggcggt gctcagtccg tgcaggtcct tcaggatcag cgccgtgcgg taccgcggcg
    10141 tcagcccggc gaggcacccc tcgacgagcc gcgtcgtctc ggctgcggcg aagaagtctc
    10201 tgcgcgccgc gacctcgggc acagcctcga gcgaggtgga cggccgcgcg gcgcgacggc
    10261 gaagatgatc gtaggaggcg ttcacggcga tcctgtagag ccacggctcg gggcgagcgc
    10321 cgtcaccggc ctgcaagggg tgcgcgtacg cccgcaggaa gacctcctgc gtgatgtcgg
    10381 cggcgtcgtc ggggtcgccg acgatgcgcg ctgccaggtt gtagatgcgg gcgtggtggt
    10441 cgcggtagaa ctgttcgaac gcccccggcc ggccgccggc gagcgcctcg agctgtgtgt
    10501 ctctatgtgc aagggcctgc acgccgaccc cctcgccgtc gccgtcgctg ccatcgctgt
    10561 cgtccatcca cgttcagtgt acccgaagga tgcacgccgc ccgccgtatg cgcaggggcc
    10621 agtgagagag gcatacgggg gcggcgcgcg tggtggggct gtgtcagtgg ccgccgccgc
    10681 ttgaaccgga accggagccg ctggtggagc cgccgccgga gccggcatcg tctggctgtc
    10741 cggtctgggt ctgggtctgc gtcccagcct gcgtctgtgt cccagcctgg gtctgcgtct
    10801 gagtctgcgc ctgcgtctgg gtctgagcct gggtctgagt ctgggtctga gtctgcgcct
    10861 gggtctgcgc acaggtctca tcacacgact cgctctgcgt gcgcaccctg gtccgcgtgc
    10921 ggtcctgagc agcgctcccg tcgcaagagc cgtcacggtc gcggtcacgg tcgcgcgcct
    10981 tcacgaggtc gcccgactcg tcggcggtgg cggagacgag gaggcagtcc tgcgtcttca
    11041 gctggtcacg cgtgcggtcc tggtccggcg tctggtcgca gctgacgtcg cagtcgttca
    11101 gctggatctt gtcctgctgc tggatctggt ccttgtcctg aagctggtcg cgcgccaggg
    11161 cgatgctgct cgccgccaag ccgacgacga cgatcagcac caggaacttc actagtctca
    11221 cgactgcctc cttctcgcgg tatccggtcc tactccctta acgcacgcgg gtgcgccggc
    11281 gttacatctt ggaggcaggg tggagacggg aggcagctgc ggccacgagt ggggtgacgg
    11341 ccgagtcaac aaggttaggt ttcgtgcgtc gtcggtaccg taacccccaa ggagaccact
    11401 ggggggccag tgccttttct cgcggctcgc cgcgtcggca tcccgtcacc acgcgacgcg
    11461 tgcagtgcag ggcggaccgg cacgcgctca gtggcgactt cttgggagcc agggtgaagg
    11521 gggccatatg cacgatcctg caggcgcacg ccgcatccga gcggtaatgg aggaccagcg
    11581 aaggcttcgc cagcgagggg gggaccgatg aagaagctcg cgctcattct gatcgcggcg
    11641 gcggcgcttg ccgtggcgtt cggcgccgcc gcatcgacga ccgcggccga aaccgtgacc
    11701 taccaagact acactcttcc cggcgtcgag ttcgactggc agaccgtggt gaacaacggc
    11761 gtccaggctc cgggtgaccc ccggaagttc aacagctaca acccgccctc gctgaacgtc
    11821 gaccggctgg tcgtgttccg tgcgcggacc aagggcggga cgggcggaca gcccgcccac
    11881 ggcgtcttca cccgcgacat gggcgtcgcg gccgcgcccg tgacgaccat cttcgaccgc
    11941 aagaccgccg tgccgcagcc gaacaacatc gtgtggcagc cgaccgacct gctcaccacg
    12001 ttcatcgagc cgccgtcctt cccgcgcatc gacatgcggt cggacaccgt cgcctcgcgc
    12061 gggaaccacc agccggtgtg ggggtacgtg cttccggacg gctcggagac gcgtgccggg
    12121 cacaccggca tctacacgga cccctacggg acgctgatca ccggagcgag caatctcggt
    12181 gcgatccccg acttcccgtt cttcgcggtt ccggggaccg acggggtcct gtttgacgtc
    12241 ttccctggcg cgcccgcggt gactgacggc gccaccgtcg tgttcaaggg gaactacacg
    12301 ttgggcacgt tgggcaggac cggcgtctac taccgcgacc tgacggactc gccgatcccg
    12361 cttctcgacg gagtcaccca gctcgcaccg gcgggcggca ctgggccggt ggtccgcatc
    12421 gccgacaccg acacgatcat ccccggcacg aggacgacca ggttcggctc caccgccccg
    12481 cccagcgcgg ccaggcgact ggcggtgttc gccggcttcg acaacgaggc gagtcccacc
    12541 aaaggcggca tctaccgggc gccgctcacc ggagcggatc cgacgctcac cacgctggtc
    12601 aagatcggcg gccaggtgcc gggcgagaag aagggtgtcg tcttcaacag gctgggtgag
    12661 ggtgtctcgt tcgacgggcg cttcgtcgcc ttctggggcg cctggggatc cgcgaccagg
    12721 aagctggtgc tgcagtgccc cgaggagggg aacgcggcac ggctcgccta ctgcagggag
    12781 ctgtatccga acggcttcgc gacgaccgtc ccggtcaacc agggcatctt cgtgtacgac
    12841 accgtcggcc gcaccacggc ggtggtcgcg aagtcgccct ccaacttctc cgacttcgtc
    12901 tactggaact tctcgggccg cgtccccggc gtcggtgagg gcggggagga cgacgacacc
    12961 ggcgagcccg cgcgctggcg ctccgccacg ttcgtcgcgg tgtccggtct ggtcgacggc
    13021 tcgctgaccg acgcgacctt ccacgtcgcc ttcaaggcga ggaccggcag gatcgtcgac
    13081 ggggcgtatg tggacccggt cgacggtatc tacctgcgca aggggccgaa caaggcgccc
    13141 atcgcggccg tggtcgagac cggcatggac ggtacgctgc tcgaccccgc ggccgtcgtc
    13201 ccgggcacgc aggtccccat cccggtcacg tcgatgggta tcgagcgcga cggcttccgc
    13261 ggcgatgccc tcgccatcaa cgtgagcatg ggcagcgagg aggccggctg ggccgggatc
    13321 tacctggcga cggtgccggc ggcgctgagg tagggcgcgt tgcggcacaa cgaacggggc
    13381 gggccgggg
//
LOCUS       DASUMX010000227         5510 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_2095016, whole genome shotgun sequence.
ACCESSION   DASUMX010000227 DASUMX010000000
VERSION     DASUMX010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 5510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5510
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2095016"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..847)
                     /locus_tag="VFZ86_10735"
     CDS             complement(<1..847)
                     /locus_tag="VFZ86_10735"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF004490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HEX5642794.1"
                     /translation="MDFVHDVIAWKRDGNELDEPRVRAFVEGVVSGAVPSYQASALLM
                     AMVLRGLSDAETLALAQAMVESGKTLDLGVLRRPVLDKHSSGGVGDKVTLVLAPLVAA
                     CGAVFGKMSGRGLGHTGGTLDKLESIPGFRVQLPQGDFLRQLERIGVAVMSQSERVVP
                     ADRLLYALRDVTGTVECDGLIAASIMAKKVASGTSAVVLDLKVGRGAFAADLDHAREI
                     GRLCRVIGTAYGRPTSCLYTAMDAPLGRTVGNRLEVEEAWQVLCGEGPGDVREVTLAL
                     AAELLP"
     gene            complement(859..1650)
                     /locus_tag="VFZ86_10740"
     CDS             complement(859..1650)
                     /locus_tag="VFZ86_10740"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642795.1"
                     /translation="MAAKPEAALRRVAAWRPHAAAVYGSGLWALPAGGCVEDEVSYDA
                     LGWPSGAVPGHQNVLRLARVPVADGRELRLALACGRPHRYEGWTDEELETPVRALAAA
                     GVSRLVLTNTCGALRPGAAVGGVVTCSAVVDLQRPPGGDAPERLSLCAHEDAARVASA
                     LGAGAAPVAGTYVSVCGPQFETPAEVAWLAGYGDVVGMSAAPEARAAAQSGVECVLLA
                     LVANVAAAVSSHEEVLAAGGRLARLLAAGLAIAVTARWPDLLDAP"
     gene            complement(1650..2783)
                     /locus_tag="VFZ86_10745"
     CDS             complement(1650..2783)
                     /locus_tag="VFZ86_10745"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEX5642796.1"
                     /translation="MTRVFVCVLDGCGAGALPDAAAYGDEGSDTLRHVLERTGVPLPN
                     LSALGLGEVVGLDLGTPRPEATYGRLVERGAGKDTTTGHWEMMGVVLARPFPTYPDGF
                     PPEVLEPFEAAIGRRVLCNRPASGTGVIEELGDEHVASGRPIVYTSADSVFQIACHED
                     VVPVPTLYEWCETARGILRGPHAVGRVIARPFDGVSGQYARTPRRRDFSLAPPGPTYL
                     DLLQERGVPVVGVGKIGEIFVQRGVDVDDHTTDNTAGIAACTRHLREMEDGLLFANLV
                     DFDQVWGHRNDVEGFAGGLAAVDAALPAWEALLEAEDVLILCADHGVDPTTESTDHSR
                     EYSPLLALGLAPGRYDGAQEDVGATAFAHLTGEEPPLSGRPIG"
     gene            complement(2784..3746)
                     /locus_tag="VFZ86_10750"
     CDS             complement(2784..3746)
                     /locus_tag="VFZ86_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase"
                     /protein_id="HEX5642797.1"
                     /translation="MTRERSSPGGRTGPPPPPADPLVREFLDYVQYERGLSPNTVTAY
                     GRDLTAFAAALAAAGAEPGSATPAQVRAYFAGSGGDGAPSSVARRTAAVRAFYAYLVR
                     EGVREDDPAVLLRTPKRPQELPRVLSVEEVEDVLAAVVRAGPLGQRDLAALELLYGCG
                     LRVSELLGLRDGDVDVEGGIVRCVGKGDKERVVPMGGAAAAAVSRYVADGRRALLKGR
                     RRPQLILNARGGPLTRQGFDYILRRVLARADMLGAASAHTFRHSFATHLLAAGADLRS
                     VQEMLGHANVATTQLYTHVTVEHLREVFLETHPRARRRPGGKEA"
     gene            complement(3764..4888)
                     /locus_tag="VFZ86_10755"
     CDS             complement(3764..4888)
                     /locus_tag="VFZ86_10755"
                     /inference="COORDINATES: protein motif:HMM:TIGR01883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX5642798.1"
                     /translation="MRAGVDAELVDLFVRLCGIASPTGAEREVADAVAAHLGGLGLGV
                     HEDGSAPVTGCGCGNLVARIAGRGEAEPLALCAHLDTVPVSGAPCVVVDEGFVRSDGA
                     TVLGADDKAAVAVLLLLARALVAEPPAGDVELVFTAGEEAGLLGAKALDLTGLTARRV
                     FVLDSDGAPGTLIVASPTQKRVTAEFQGVAAHAGIAPEKGRNAIVAAARAIGAMRLGR
                     IDDETTANIGVVNGGSASNVVAEHCVVAGEARSLDAAKVARLAEEMVQAMAAEAGAAG
                     IDVEIDVSEGFRGYRHAPESPLLEIGARAAALAGLEVRLAAGGGGSDANVFNAAGLPA
                     LTLGVGFENAHSPRERMSLERLGELATMAAAVVRAAATAA"
     gene            complement(4873..>5510)
                     /locus_tag="VFZ86_10760"
     CDS             complement(4873..>5510)
                     /locus_tag="VFZ86_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003388456.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEX5642799.1"
                     /translation="PATVNDRLCTADGVIVPGGFGQRGVEGKIDAIKYCRVNKVPFLG
                     VCLGMQCAVAEFARDVCDMAGANSTEFDPETAYPVIDLLPEQKNVEDMGGTMRLGASH
                     VKLVPGTRAQVAYDAGEVWERHRHRYEVNNDLRPQLEAAGLVISGTTPDGRLVEICEL
                     PDHPWFVASQFHPEFKSRPTRPAPLFRDFVGAAARLRSERDDELEDVCAPA"
BASE COUNT          716 a         2135 c         1993 g          666 t
ORIGIN      
        1 gcggcagcag ctcggcggcc agcgccagtg tgacctcgcg cacgtcgccg gggccttctc
       61 cgcacagcac ctgccacgcc tcctccacct cgagccggtt gcccacggtc cgccccagcg
      121 gcgcgtccat ggccgtgtac aggcacgagg tcgggcgccc gtaggcggtc ccgatcaccc
      181 ggcagaggcg gccgatctcc cgggcgtggt ccaggtcggc cgcgaacgct ccccgcccca
      241 ccttgaggtc gaggaccacc gccgacgtgc cgctcgcgac cttcttggcc atgatgctcg
      301 cggcgatgag cccgtcgcac tccaccgtcc cggtgacgtc ccgcagcgca tacagcagcc
      361 ggtccgccgg gacgacgcgt tcgctctggc tcattaccgc cacgccgatg cgctccagct
      421 ggcggaggaa gtccccctgc ggcagctgca cgcggaagcc ggggatcgac tcgagcttgt
      481 ccagcgtccc gccggtgtgc cccaggccgc ggccggacat cttgccgaag acggcgccgc
      541 aggccgcgac gagcggcgcc agcaccagcg tgaccttgtc ccccaccccg ccgctcgagt
      601 gcttgtcgag caccggacgg cgcaggacgc cgaggtccag cgtcttgccg ctctcgacca
      661 tcgcctgcgc cagcgcgagc gtctcggcgt cgctgagccc gcgcagcacc atcgccatga
      721 gcagcgcgct cgcctggtag gagggcacgg ccccgctgac cacgccctcg acgaaggcgc
      781 gcacgcgcgg ctcgtcgagc tcgttgccgt cgcgcttcca ggcgatcacg tcgtgcacga
      841 agtccacgtc actccccctc agggcgcgtc gagcagatcc ggccagcgcg ccgtcacggc
      901 gatcgcgaga ccggcggcga gcagccgggc gaggcggccg ccggcggcga gcacctcctc
      961 gtgcgagctc accgcggccg cgacgttggc caccagcgcc agaagaacgc actctacgcc
     1021 gctctgggcg gcggcgcgag cctccggcgc cgccgacatc cccacgacat cgccgtagcc
     1081 cgcgagccag gcgacctccg ccggcgtctc gaactgcggg ccgcacaccg agacgtacgt
     1141 gccggcgacg ggggccgcgc cggcgcccag ggcggaggcc acgcgggcgg cgtcctcgtg
     1201 cgcacacagg gacaggcgct ccggcgcgtc tccacccggc ggacgctgca ggtccacgac
     1261 ggcggagcag gtgacgaccc cgccgacggc ggcgcccggc cgcaacgcgc cgcacgtgtt
     1321 ggtcagcacc agccgggaga cgccggccgc ggcaagcgcc cgcaccggcg tctcgagctc
     1381 ctcgtccgtc cagccctcgt agcggtgcgg ccgcccgcag gccagggcga gccggagctc
     1441 gcgcccgtcg gcgacgggca cgcgcgcgag ccgcaggacg ttctggtggc cgggcaccgc
     1501 gcccgacggc cagccgagcg cgtcgtacga gacctcgtcc tcgacgcacc cgcccgcggg
     1561 caacgcccag agcccggagc cgtagaccgc ggcggcatgc ggccgccagg ccgccacgcg
     1621 ccgcagagcg gcctcaggct tcgccgccat cagccgatcg gacgcccgga gagcggcggt
     1681 tcctcgccgg tcaggtgcgc gaacgccgtc gcgcccacgt cctcctgggc gccgtcgtac
     1741 cggcccggcg ccagccccag ggccagcagc ggcgagtact cgcgcgagtg gtcggtgctc
     1801 tccgtggtgg ggtccacgcc gtggtcggcg cacaggatca gcacgtcctc ggcctcgagg
     1861 agcgcctccc acgcgggcag cgccgcgtcc accgcggcga ggccgccggc gaagccctcc
     1921 acgtcgttgc ggtggcccca cacctggtcg aagtcgacga ggttggcgaa cagcagcccg
     1981 tcctccatct cgcgcaggtg ccgggtgcag gcggcgatgc cggccgtgtt gtcggtggtg
     2041 tggtcgtcca cgtcgacgcc gcgctgcacg aagatctcgc cgatcttgcc cacgccgacg
     2101 acggggacac cccgctcctg aagcaggtcg aggtaggtcg ggcccggcgg cgccagcgag
     2161 aagtcgcgcc gccgcggcgt gcgcgcgtac tggccggaga cgccgtcgaa aggccgcgcg
     2221 atgacgcgcc ccacggcgtg cggcccccgc aggatgccgc gcgcggtctc gcaccactcg
     2281 tagagcgtcg gcaccggcac gacgtcctca tggcaggcga tctggaacac cgagtcggcg
     2341 gacgtgtaga cgatgggccg gccgctggcg acgtgctcgt cgcccagctc ctcgatcacg
     2401 ccggtaccgg aggccggccg gttgcagagc acgcgccggc cgatcgccgc ctcaaatggc
     2461 tccagcacct cgggcggaaa gccgtccggg taggtcggga acggccgcgc gagcaccacc
     2521 cccatcatct cccagtggcc cgtcgtggtg tccttgccgg cgccgcgctc caccaggcgc
     2581 ccgtaggtcg cctccgggcg cggcgtcccg aggtccagcc cgacgacctc accgagtccg
     2641 agggcggaga ggttggggag cgggacgccg gtgcgctcga gcacgtgccg cagcgtgtcc
     2701 gacccctcgt cgccgtaggc ggcggcgtcg ggcagcgccc ccgcgccgca gccgtccagc
     2761 acgcacacga acacgcgcgt catctacgcc tccttgccgc cgggccgtcg ccgtgcccgg
     2821 gggtgcgtct ccaggaacac ctcgcggagg tgctccacgg tcacgtgagt gtacaactgg
     2881 gtggtcgcca cgttggcgtg gccgagcatc tcctgcacgc tgcgcaggtc ggccccggcg
     2941 gcaagcaggt gcgtggcgaa cgagtgccgg aacgtgtgcg cgctggccgc accgagcatg
     3001 tctgcgcggg cgagcacgcg gcgcaggatg tagtcgaacc cctggcgcgt gagcggcccg
     3061 ccgcgcgcgt tcaggatgag ctgcgggcgg cggcggcctt tcaggagagc ccgccgcccg
     3121 tcggcgacgt accggctcac ggccgccgcg gccgcgccgc ccatgggcac cacgcgctcc
     3181 ttgtccccct tgccgacgca gcgcacgatc ccgccctcga cgtccacgtc cccgtcgcgc
     3241 aggcccagca actcgctcac gcgcagcccg cagccgtaga gcagctcgag cgcggcgagg
     3301 tcgcgctgtc ccagaggccc ggcccgcacc accgccgcca ggacgtcctc gacctcctcg
     3361 acggagagga cgcgcggcag ctcctgcggc cgtttgggcg tgcgcagcag gaccgccggg
     3421 tcgtcttcgc gcacgccctc gcgcaccaga taggcgtaga aggcgcgcac cgccgccgtc
     3481 cggcgcgcca cgctggacgg cgcgccgtcg ccgccggagc cggcgaagta ggcgcggacc
     3541 tgcgcgggcg tggcgcttcc cggctccgcg ccggccgccg cgagggccgc cgcgaacgcg
     3601 gtgaggtcgc ggccgtaggc ggtcacggtg ttggggctga ggccgcgctc gtactgaacg
     3661 tagtcgagga actcccggac caggggatcg gcgggaggtg gcggaggccc ggtccgcccg
     3721 cccggagaac ttcgttcgcg ggtcacgcgc cgccccgggg ccgctacgcg gccgtcgcgg
     3781 cggcgcgcac cacggccgcc gccatggtcg ccagctcgcc gagccgctcg aggctcatgc
     3841 gctcgcgcgg cgagtgcgcg ttctcgaagc cgacgccgag cgtgagcgcc ggcaggccgg
     3901 cggcgttgaa gacgttcgcg tccgagccgc cgcccccggc ggccaggcgg acctcgagac
     3961 ccgcgagcgc cgccgcccgg gcgccgatct ccagcaacgg gctctcgggc gcgtgccggt
     4021 agccgcggaa cccttcgctg acgtcgatct cgacgtcgat gccggccgct cccgcctccg
     4081 ccgccatcgc ctgcaccatc tcctcggcga gcctcgcgac cttggcggcg tccaggctgc
     4141 gcgcctcacc ggccaccacg cagtgttccg cgaccacgtt cgaggcgctg cctccgttca
     4201 ccacgccgat gttcgccgtg gtctcgtcgt cgatgcggcc cagacgcatc gcgccgatgg
     4261 ctcgcgccgc ggcgacgatg gcgttgcgcc ctttctcggg ggcgatgccg gcgtgcgccg
     4321 cgaccccctg gaactccgcc gtcacgcgct tctgcgtcgg cgaggcgacg atgagcgtgc
     4381 ccggcgcgcc gtcgctgtcg agcacgaaca cccgccgtgc cgtgaggccg gtcaggtcca
     4441 gcgccttggc gcccagcagg ccggcctctt cccctgcggt gaagacgagc tcgacgtcgc
     4501 ccgcgggcgg ctcggcgacg agcgcgcggg cgagcagcag caggacggcg acggcggcct
     4561 tgtcgtcggc gccgagcacc gtggcgccgt cgctgcgcac gaacccctcg tcgacgacca
     4621 cgcacggcgc gcccgagacc ggcacggtgt cgaggtgggc gcacagggcg agaggctccg
     4681 cctccccccg gccggcgatg cgggcgacca ggttgccgca gccgcagccg gtgacgggag
     4741 cgctgccgtc ctcgtggacg ccgaggccga ggccgccgag atgcgccgcg acggcgtcgg
     4801 cgacctcccg ctcggccccg gtcggcgacg cgatgccgca gagccgcacg aagaggtcga
     4861 cgagctccgc gtctacgccg gcgcgcacac gtcctccagc tcgtcgtcac gctcgctgcg
     4921 caggcgggcg gcggcaccca cgaagtcgcg gaagagcggc gccggccgcg tgggccgcga
     4981 cttgaactcg gggtggaact gggaggcgac gaaccagggg tgatcgggca gctcgcagat
     5041 ctcgacgagc cgtccgtccg gagtggtgcc gctgatcacg aggccggcgg cctcgagctg
     5101 cgggcgcagg tcgttgttga cctcgtagcg gtggcggtga cgctcccaga cctcgccggc
     5161 gtcgtaggcg acctgggccc tggtgccggg gaccagcttc acgtgggagg cgccgagccg
     5221 catggtgccg cccatgtcct ccacgttctt ctgctccggc aacaggtcga tgaccgggta
     5281 ggcggtctcg gggtcgaact cggtggagtt ggcgccggcc atgtcgcaga cgtcgcgcgc
     5341 gaactcggcg acggcgcact gcatgcccag gcacacgccg aggaacggga ccttgttcac
     5401 gcggcagtac ttgatggcgt cgatcttgcc ctccaccccg cgctgcccga aaccgccggg
     5461 gacgatcacg ccgtcggccg tgcagagccg gtcgttcacc gtcgccggcg
//
LOCUS       DASUMX010000228         4769 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_226001, whole genome shotgun sequence.
ACCESSION   DASUMX010000228 DASUMX010000000
VERSION     DASUMX010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4769
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226001"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(273..1346)
                     /locus_tag="VFZ86_10765"
     CDS             complement(273..1346)
                     /locus_tag="VFZ86_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEX5642800.1"
                     /translation="MTANASEPLHWAVFFAIVAIALAIDLGFVGRKGDVVSTRTAYAW
                     SAVWISLGIGFSGLVYLWYGSEAAQQYLAGYLIEESLSIDNLFVFLLVFGSLGIALQY
                     QRKVLFWGILGAMLMRGVFIVAGVSLINRFEWIVYIFGAVLVYTAYRLVRSSGVQVDP
                     EKNPLVRFARRLFPVAADYKGRRFVIRRGGKRFITPLVLALVAIESADLVFAIDSVPA
                     VLAVSNITFVVFTSNIAAILGLRSLYFALAGSLQRFEYLSWGLAVVLGFVGVKMLISH
                     WLHIPVLISLGVIVVAVGTSVIVSLVLTRGAEPDADVLVAAGRGDLVEKRGEAPEEPD
                     APEGGDAPANEDAPAHGDTAASR"
     gene            complement(1566..1730)
                     /locus_tag="VFZ86_10770"
     CDS             complement(1566..1730)
                     /locus_tag="VFZ86_10770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642801.1"
                     /translation="MRDPQTLPRTCGLLTCPECGLELETLLLRCPRCLATIPLGCSGN
                     CRECGKDDGR"
     gene            complement(1751..3622)
                     /gene="feoB"
                     /locus_tag="VFZ86_10775"
     CDS             complement(1751..3622)
                     /gene="feoB"
                     /locus_tag="VFZ86_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HEX5642802.1"
                     /translation="MSAATATAPCCPSPTVDAPAGARRIVLVGNPNVGKSLFFNAFTG
                     LYVDVSNYPGTTVAVSSGHMGDDVVLDTPGIYGVSSFNDEETVARDIVMEADVVLNVV
                     DAVHLERDLFLTQQIIDMGIPMVVALNMADEARAKGVEIDVEELSSRLAVPVVPTVAV
                     RREGFDQVREALADARPGRSDPELQERLAALLDRTGTRPEALLVLEADPFVSARTGVS
                     VEGQRDAIYQDRRRRVDAVVAAAVRHPGSRTDLGARLGRALVRPLVGVPVLALTLFAM
                     YEVIGVFVAGTVVGFTEGTVMQGYVEPFLRGAVERVTGSSGVAYSILAGEFGVVTMTV
                     TYILGLLLPLVVAFYLLMSTLEDSGYLPRIAALSDRLMSGIGLNGRAIIPMILGFGCI
                     TMATMTTRILGSERERRIATALMAFAIPCSAQLGVVVALLAAAGGGTIALAYGAIMVL
                     IFGLIGLALDRVLPGKSTDLLIDLPPLRLPRPRNVLTKTYHKTWMFLKEVSLYFVAGA
                     LLLSLLEVTGALQGLQGLLAPLTVGWLGLPSAASNAFVMGFVRRDFGAAGLFDLGLGG
                     TQILVALVTITLFVPCIASVLIIMKERGKAFTAAVWFGSVGLAFFVGGVVAKLAGVL"
     gene            complement(3619..3846)
                     /locus_tag="VFZ86_10780"
     CDS             complement(3619..3846)
                     /locus_tag="VFZ86_10780"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HEX5642803.1"
                     /translation="MTLLDARPGRPVRILKIADDQARAQCVRFGIGEGTIIDSAETLP
                     LGPVLVRHCRQEICVGRRLAGRVLVEDAVGA"
     gene            4010..4417
                     /locus_tag="VFZ86_10785"
     CDS             4010..4417
                     /locus_tag="VFZ86_10785"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5642804.1"
                     /translation="MQNLIVWADIPATDLDRAMAFYGAVLQRPFVKVEGMEGIALPGP
                     DEDAPAGDAAEQQMPVAFDLFVGEQARPSEDGSTIYLESNGDAQGMLQRAADAGGEIL
                     MPATFMGDMVGTMGFFRDSEGNRVGVHQNGPLT"
     gene            complement(4457..>4769)
                     /locus_tag="VFZ86_10790"
     CDS             complement(4457..>4769)
                     /locus_tag="VFZ86_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013979865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642805.1"
                     /translation="SNDNASYWQGYLGYPVLAVLLARGVLHADAAAVDAMAGVPWYDL
                     NTRFKRDYEAAVARVLGELSAHGGDPALVEREVAAVLDQLAALDLRRASRGRRPPAAH
                     D"
BASE COUNT          803 a         1634 c         1698 g          634 t
ORIGIN      
        1 ccgtggccat ctgtgcggcg atcgacggcg agacgagccg tccgcgcact gcggtcacgc
       61 gctctgggtc gaccacgtgc acgtcgcagt ggtcgagcag gtcctcgtcg tccttgccgc
      121 agatcaccgg cgcctcgagc cgaccgtcgg tcatcgccct ctcctctgaa catatgaaca
      181 gatgttcata tgttacccgg agcgcgacct cccgacaagc ccgtccgttg tctgccggcc
      241 ttacgtgggt cgggcgccgc gccccgtccg cctcacctgc tcgcggccgt gtccccgtgc
      301 gccggtgcgt cctcattcgc cggcgcgtcc ccaccctccg gcgcatccgg ctcctccggc
      361 gcttcgccgc gcttctcgac gaggtcgccg cggccggcgg ccacgagcac gtcggcgtcc
      421 ggctccgcgc ctcgcgtgag gaccagcgag acgatcaccg acgtccccac cgcgaccacg
      481 atgacaccga gcgagatgag cacggggatg tgaagccagt ggctgatcag catcttgacg
      541 ccgacgaacc ccagcacgac ggcgagcccc cagctgaggt actcgaagcg ctgcaggctg
      601 ccggcgagcg cgaagtagag cgagcgcagc ccgaggatcg cggcgatgtt gctggtgaag
      661 accacgaatg tgatgttgga gacggcgagc acggccggga ccgagtcgat ggcgaacacg
      721 aggtcggccg actcgatggc gaccagggcg agcaccagcg gcgtgatgaa cctcttgccg
      781 ccgcggcgga tgacgaagcg gcggcccttg tagtccgccg ccacggggaa gaggcgccgc
      841 gcgaagcgca ccagcgggtt cttctccggg tccacctgta cgccgctcga gcgcacgagc
      901 cggtaggccg tatacacgag tactgcgccg aagatgtaga cgatccattc gaagcggttg
      961 atgaggctca ccccggcgac gatgaagacg ccgcgcatga gcatcgcccc gaggatgccc
     1021 cagaacagca ccttgcgctg gtactgcagg gcgatgccga gcgagccgaa gacgagcagg
     1081 aagacgaaga ggttgtcgat gctgagcgac tcttcgatga gatagccggc gaggtactgc
     1141 tgcgccgcct cgcttccgta ccacaggtac acgaggccgg agaagccgat gcccagcgag
     1201 atccagacgg cgctccaggc gtaggcggtg cgcgtgctga cgacgtcgcc cttgcggccc
     1261 acgaacccga ggtcgatcgc cagggcgatg gcgacgatgg cgaagaagac cgcccagtgc
     1321 agcggttcgc tcgcgttggc ggtcactcca cgtcctccac tcgtcgtctc ggggcggcag
     1381 gcaaaaagac ctccggcacg cctgtgccga aggtcttgcc gttcgccggc gtggccgggg
     1441 tctcgcgacc cgtgatgacg accgcgtcac tcgccctgtc gggcgagccg gctactcccc
     1501 tacgggccgc gagcgtaccc gccggacagt ggcagggtca accgagagcc gtcgacgcgg
     1561 gcgcgtcagc gcccgtcgtc cttgccgcac tccctgcagt tgccgctgca gcccaacggg
     1621 atcgtggcga ggcagcgagg gcaacggagc aggagggtct cgagctcgag tccgcactcg
     1681 gggcaggtga ggaggccgca cgtccgcggc agggtctggg ggtcgcgcat cgcccccggc
     1741 cggggcatcg tcacagcacc ccggcgagct tcgcaacgac gccgccgacg aagaaggcca
     1801 gtcccacgct gccgaaccag accgcggcgg tgaaggcctt gccgcgctcc ttcatgatga
     1861 tgagcaccga ggcgatgcag ggcacgaaca gcgtgatcgt caccagcgcg acgaggatct
     1921 gggtgccgcc gaggccgagg tcgaacaggc cggcggcgcc gaagtcgcgc cgcacgaagc
     1981 ccatcacgaa ggcgttcgac gcggccgagg gaaggccgag ccagccgacg gtgaggggcg
     2041 cgaggagacc ctgcaggccc tggagcgcgc ccgtcacctc gagcaggctg agcagcagcg
     2101 cgccggcgac gaagtacagg ctcacctcct tcaggaacat ccacgtcttg tggtacgtct
     2161 tggtgagcac gttgcgcggg cgcggcaggc gcagcggcgg caggtcgatc agcaggtcgg
     2221 tcgacttgcc gggcaggacg cggtcgagcg ccagaccgat gagcccgaag atgaggacca
     2281 tgatcgcccc gtaggcgagc gcgatcgtgc cgccgccggc cgccgcgagc agagccacga
     2341 ccacgccgag ctgcgccgag caggggatcg cgaaggccat gagggccgtc gcgatccgcc
     2401 gctcgcgctc gctgccaagg atgcgggtgg tcatcgtcgc catcgtgatg cacccgaagc
     2461 ccaggatcat ggggatgatg gcgcgcccgt tgaggccgat gcccgacatc aggcggtctg
     2521 agagcgccgc gatgcgcggc aggtaaccgg agtcctcgag ggtcgacatc agcaggtaga
     2581 aggcgacgac gagcgggagg agcagcccga ggatgtaggt caccgtcatc gtgaccacgc
     2641 cgaactcacc cgcgaggatg ctgtaggcga cgccggacga tcccgtcacg cgttcgaccg
     2701 cgccgcgcag gaacggctcc acgtagccct gcatcacggt gccttcggtg aagccgacca
     2761 cggtgccggc cacgaacacg ccgatgacct cgtacatggc gaagagcgtg agggcgagca
     2821 ccgggacccc gacgagcggg cgcaccaggg cgcgccccag cctggctccc aggtcggtcc
     2881 tgcttcctgg gtgacggacg gcggcggcga ccacggcgtc cactcgccgc cgccggtcct
     2941 ggtagatcgc gtcgcgctgt ccctcgacgc tgacgccggt ccgcgcggag acgaacggat
     3001 cggcctcgag aacgagcagc gcctccggcc tggtccccgt gcggtcgagc agggcggcga
     3061 ggcgctcctg cagctccggg tcggagcgcc cggggcgcgc gtccgcaaga gcctcgcgca
     3121 cctggtcgaa gccctcgcgg cgcacggcga cggtggggac cacggggacg gcgagccggc
     3181 tggagagctc ctcgacgtcg atctccacgc ccttggcccg tgcctcgtcc gccatgttga
     3241 gcgcgacgac catggggatg cccatgtcga tgatctgctg ggtgaggaag aggtcgcgct
     3301 ccagatggac cgcgtcgacc acgttgagga cgacgtccgc ttccatgacg atgtcgcgcg
     3361 ccaccgtctc ctcgtcgttg aacgaggaca cgccgtagat gccgggcgtg tccagcacca
     3421 cgtcgtcacc catgtgtccg ctggagacgg cgacggtcgt gcccgggtag ttggagacgt
     3481 cgacatagag gccggtgaag gcgttgaaga agagcgactt gccgacgttg gggttcccga
     3541 caaggacgat cctgcgagcg ccggctgggg cgtcgacggt gggcgacgga cagcagggcg
     3601 ccgtcgcggt cgctgcgctc acgcgcccac cgcgtcctcg acgaggaccc gacccgccag
     3661 cctgcgcccc acgcagatct cctgccggca gtggcgcacg agcaccggcc ccaacgggag
     3721 cgtctcggcg gagtcgatga tcgttccttc accgatgccg aagcgcacgc actgggcgcg
     3781 cgcctggtcg tcggcgatct tgaggatgcg taccggccgg ccgggccggg cgtcgagcag
     3841 ggtcattcgt cgtcctttcg gagggagtcc gacagggatg acttagggaa gcctaatcag
     3901 cagaaacagg atagccatgt aggaaaccaa cgtcaaacgc attgaccccg gtcgccggcg
     3961 ggaatcagtc caccacgtca ggcaaccgct gagcagcgga ggacacgaca tgcagaatct
     4021 catcgtctgg gccgacatcc cggccacgga cctcgaccgc gccatggcct tctacggcgc
     4081 cgtcctgcag cgcccgttcg tcaaggtgga gggcatggag ggcatcgccc tgcccggccc
     4141 cgacgaggac gctccggccg gcgatgcggc ggagcagcag atgccggtgg cgttcgacct
     4201 cttcgtcggc gagcaggcga ggccgagcga ggacggcagc accatctatc tggagagcaa
     4261 cggcgacgcc cagggcatgc tccagcgcgc ggccgacgcc ggcggcgaga tcctcatgcc
     4321 ggccacgttc atgggcgaca tggtcggcac catgggcttc ttcagggaca gcgagggcaa
     4381 ccgcgtcggc gtgcaccaga acggtccgct gacctgaccg gacggtgccg gcgatcggcc
     4441 gcgactgccc gtcgcctcag tcatgcgcgg ccggcgggcg gcggccgcgg gacgcgcgcc
     4501 gcaggtcgag ggccgcgagc tggtccagca cggcggcgac ctcgcgctcc acgagcgccg
     4561 ggtcgccgcc gtgcgccgac agctcgccga gcacgcgcgc gacggccgcc tcgtagtcgc
     4621 gcttgaaccg cgtgttgagg tcgtaccagg ggacgccggc catggcgtcg accgcggccg
     4681 cgtcggcatg gagcacccca cgtgcgagca gtacggcgag gaccgggtag ccgaggtacc
     4741 cctgccagta cgaggcgttg tcgttactc
//
LOCUS       DASUMX010000229         4120 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1663861, whole genome shotgun sequence.
ACCESSION   DASUMX010000229 DASUMX010000000
VERSION     DASUMX010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4120
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1663861"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            164..925
                     /locus_tag="VFZ86_10795"
     CDS             164..925
                     /locus_tag="VFZ86_10795"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX5642806.1"
                     /translation="MTSDDEQRIDTGGAGDTAAEVHPAEDPPPEGPAHGAVRYSRRRV
                     IVLGGAVAAGVAAVIAGVRAFGGPSEVADKVGGPVADQFGTFPVRSVESVPDLTPEEW
                     VVTVDGLVESPLTVDRATWAGLERKAETVDFHCVEGWSVDDVRWGGVAPAVLLDQAGV
                     RPEAKYAVFHAHGGVYLSTLPLELVRDSQSLLADSLSGAPLPPKHGGPLRLVVPKQLG
                     YKSVKWVERIELTSQVKTGYWEGNGYPEDAPVPGS"
     gene            1121..1285
                     /locus_tag="VFZ86_10800"
     CDS             1121..1285
                     /locus_tag="VFZ86_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005843250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HEX5642807.1"
                     /translation="MTKYVCTVCDYVYDPAVGDPDNGIEPGTAFEDLPDDWVCPECGV
                     DKEEFEAVQD"
     gene            1288..2034
                     /locus_tag="VFZ86_10805"
     CDS             1288..2034
                     /locus_tag="VFZ86_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5642808.1"
                     /translation="MGVLELRDVTLELGGRRILNGLTVDFWEGHVHALVGPNGAGKST
                     LAGAIMGLPDYRHLSGEILLDGEPIQDLPVDERARRGITLAWQEPARFEGLRVDRFIL
                     AGARERSAATVERVLGRVGLDPEKYATRAVDKTLSGGERKRVELASILAMEPRLVLMD
                     EPDSGIDVEALTRIFDALADFKERGATVIMITHSQEVLKHAEHAFLLCDGRIIDKGAV
                     GRIDDYFGDNCLSCGHTNVPTRNEMAGVAE"
     gene            2031..2981
                     /locus_tag="VFZ86_10810"
     CDS             2031..2981
                     /locus_tag="VFZ86_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HEX5642809.1"
                     /translation="MSSPTREQVETARELLASLGDFGGHIKGDDIAHLEVHGNEVVGA
                     HLVPGLQVDVDELDDGIEAWIHLDKGARIAKPVHLCFGMLPAAGLQRILMHVEAEEDS
                     FASVQAHCTFPNAVDITHRMDADVVVGPGATYQYFERHLHGAEGGVIVVPKTRVHVKE
                     GGRFKTEFELIKGMAGVIDFDYEVDCDAHSTLDMIARIIGRGRDKIRINETGHLNGEG
                     ATAVLQSHLALRDDAFAEIYNTLTASAPHARGHVDCKEIVQGNAVAKAVPIVEVSHAL
                     AHVTHEAAIGSVDSKQLQTLMSRGLDEDAATDLIIEGLLG"
     gene            complement(3036..>4120)
                     /locus_tag="VFZ86_10815"
     CDS             complement(3036..>4120)
                     /locus_tag="VFZ86_10815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642810.1"
                     /translation="PSASAPLVQGIQGTYTWRPVDGPGEDGTFGALAGGDAGGKASVP
                     EDETVFSYHPPESAYDATTRTETTVGGNGGRAALATRTVGQWPPVWRVATHSPLDYQG
                     LAAIVRTAVEDGDEAVGIKPVKDDERAVWRAAMKLDGKEVNVVVDQQTGIVTWCSDDE
                     ATFTATVDWDAPPPADTTYSVDVPAGTETETIGDTDAYEPSPAAAGRTAGYAPLVSDL
                     APDGYGLQSVATVADGTQVYDWTGHPTEAGPISPPRELVIGQLYTRGLSWFTLEQLGP
                     KATDFYGAGLKDWPASSSDERLSLQQTTLQYGRLKGATAYTWYQESGPSLFAAGRRRA
                     VFITGALTRQELIAFAEGLQPVAPPQ"
BASE COUNT          626 a         1433 c         1503 g          558 t
ORIGIN      
        1 cagccgcccg tgaggggcgg tgagacggag gtgcggggcg gcggccgacg aggccgccgc
       61 ccccgcgtac gggagcgacg cagcgacgct caccccgggg ccttggctcg tttcaggatg
      121 ggaatagtcc ggttgagtga gacggacgag catgaggccc gcgatgacga gcgacgacga
      181 gcagaggatc gataccggcg gcgccggcga cacggcggcg gaagtccatc cggccgagga
      241 cccgccgccc gaagggccgg cgcacggcgc cgtgcggtac tcgcgccgcc gcgtgatcgt
      301 cctcggcggc gcagtcgctg cgggagtcgc cgccgtgatc gccggcgtcc gtgcgttcgg
      361 cgggccttcc gaggtggccg acaaggtcgg cggcccggtg gccgaccagt tcggtacctt
      421 ccccgtgcgc agcgtcgagt ccgtgccgga cctgacgccc gaagagtggg tcgtcacggt
      481 ggacggcctg gtcgagtcgc cgctgaccgt cgacagggcc acctgggccg gcctggagcg
      541 caaggccgag acggtcgact tccactgcgt ggagggctgg agcgtcgacg acgtccgctg
      601 gggcggcgtg gcgccggccg tcctgctcga ccaggccggc gtgaggcccg aggcgaagta
      661 cgccgtcttt cacgcgcacg gcggcgtcta cctgagcacg ctgccgctcg agctcgtgcg
      721 cgactcccag tccctgctcg cggattctct gagcggcgca ccgctgccgc ccaagcacgg
      781 cggtccgctg cgcctcgtcg tccccaagca gctgggctac aagagcgtca agtgggtgga
      841 gcggatcgag ctcaccagcc aggtgaagac agggtactgg gaggggaacg gctacccgga
      901 ggacgcgccg gtccccggga gctgaggctg gacggaaggc cccggcgccg gaatcccatc
      961 ctcgaacggg tcaggcaaag cgccgtcctc gggtttcggg acgcagggct cgcgggcacc
     1021 gttctctgcg gacaggagag agaccgcccg gcgcgataca ctggagcccg cgccgcaccg
     1081 tccgcgctcc cgagcaaagc gatcctccag gaggccgagg atgaccaagt acgtctgcac
     1141 cgtctgcgac tacgtgtacg accccgcggt aggggacccc gacaacggca tcgagccggg
     1201 cacggcgttc gaggacctgc ccgacgattg ggtctgcccc gagtgcggcg tggacaagga
     1261 agagttcgag gccgtccagg actgagaatg ggcgtccttg agctgcggga cgtcacgctc
     1321 gagctcggcg gccggcgcat cctcaacggc cttaccgtcg acttctggga ggggcacgtc
     1381 cacgccctgg tggggcccaa cggcgccggc aagagcacgc tggccggcgc gatcatgggc
     1441 ctgcccgact accggcacct gagcggcgag atcctgctcg acggcgagcc catccaggac
     1501 ctgccggtgg atgagcgggc gcgccgcggc atcacgctcg cgtggcagga gccggcgcgg
     1561 ttcgaggggc tccgggtgga ccggttcatc ctggccggag ccagggagcg gagcgccgcg
     1621 acggtcgagc gggtgctggg ccgcgtgggc ctggaccccg agaagtacgc gacgcgcgcc
     1681 gtggacaaga cgctgtccgg cggcgagcgc aagcgcgtgg agctcgccag catcctcgcc
     1741 atggagcccc gcctggtgct catggacgag cccgactcgg gcatcgacgt ggaggcgctc
     1801 acgcgcatct tcgatgccct cgccgacttc aaggagcgcg gtgccacggt catcatgatc
     1861 acccacagcc aggaggtgct caagcacgcc gagcacgcct tcctgctctg cgacgggcgc
     1921 atcatcgaca agggcgccgt gggccgcatc gacgactact tcggcgacaa ctgcctgagc
     1981 tgcggccaca ccaacgtgcc cacgaggaac gagatggcgg gggtggccga gtgagctcgc
     2041 ccaccaggga acaggtcgag accgcgcgcg agctgctcgc cagcctcggc gacttcggcg
     2101 ggcacatcaa gggcgacgac atcgcccacc tcgaggtgca cggcaacgag gtggtcgggg
     2161 cgcatctcgt ccccggcctg caggtcgacg tcgacgagct cgacgacggc atcgaggcct
     2221 ggatccacct ggacaagggc gcgcgcatcg ccaagccggt gcacctgtgc ttcggcatgc
     2281 tgcccgccgc tggcctgcag cgcatcctca tgcacgtgga ggcggaggag gacagcttcg
     2341 ccagcgtgca ggcgcactgc accttcccca acgccgtcga catcacccac aggatggacg
     2401 ccgacgtggt cgtcggaccc ggcgccacct accagtactt cgagcgccac ctgcacggcg
     2461 cggagggcgg cgtgatcgtg gtgcccaaga ccagggtgca cgtcaaggag ggcggccgct
     2521 tcaagacgga gttcgagctc atcaagggca tggccggcgt catcgacttc gactacgagg
     2581 tcgactgcga cgcgcacagc acgctcgaca tgatcgcgcg catcatcggc cgcggccgcg
     2641 acaagatccg catcaacgag accggccacc tgaacggcga aggtgcgacc gccgtgctgc
     2701 agtcgcacct ggcgctgcgc gacgacgcct tcgccgagat ctacaacacg ctcacggcga
     2761 gcgcgccgca cgcccgcggc cacgtcgact gcaaagagat cgtgcagggc aacgcagtgg
     2821 ccaaggccgt gccgatcgtc gaggtgagcc acgcgctggc gcacgtgacg cacgaggccg
     2881 cgatcggcag cgtcgacagc aagcagctgc agacgctgat gagccgcggg ctcgacgaag
     2941 acgcggccac cgacctgatc atcgaggggc tgctgggctg acgcgcgtca ggccggggcg
     3001 cgctcgcctg agccccggcg tgactgacac cgcgcttatt gcggcggcgc gactggctgc
     3061 agcccctcgg cgaacgcgat gagttcctgg cgggtgagcg cgccggtgat gaacactgcc
     3121 cggcgccgcc cggcggcgaa cagcgacggc ccgctctcct ggtaccacgt gtaggccgtc
     3181 gcgcccttga gcctcccgta ctgcagcgtc gtctgctgga gcgagagcct ctcgtcggat
     3241 gatgaggccg gccagtcctt gagaccggcg ccgtagaagt cggtcgcctt ggggccaagc
     3301 tgctccagcg tgaaccagct gaggcctcgc gtgtacagct ggccgatgac cagctcccgc
     3361 ggcggggaga tggggcccgc ttctgtcggg tgcccggtcc agtcgtagac ctgcgtgccg
     3421 tccgcgaccg tggcgacact ctggagcccg tagccgtcag gcgccaggtc tgagacgagc
     3481 ggagcgtagc cggcggtgcg acccgccgcc gccggtgacg gctcgtaggc gtccgtgtcc
     3541 ccgatggtct ccgtctctgt gcccgccggc acgtcgacgc tgtaggtcgt atcggccggc
     3601 ggcggcgcgt cccagtcgac cgtcgcggtg aaggtggcct cgtcgtcgct gcaccaggtg
     3661 acgatgccgg tctgctggtc cacgacgacg ttcacctcct tgccgtccag cttcatcgcc
     3721 gcccgccaga ccgcgcgctc atcgtccttg accggcttga tgccgacggc ttcgtcgccg
     3781 tcctcgaccg cggtgcgcac gatcgcggcc agaccctggt agtccagcgg gctgtgcgtg
     3841 gcgacgcgcc agaccggcgg ccactggccg acggtgcggg tggcgagggc ggcgcgtccg
     3901 ccgttgccgc cgacggtggt ctcggtgcgc gtcgtggcgt cgtaggcaga ctcgggcggg
     3961 tgatagctga agacggtctc gtcctccggg acgctcgcct tcccgcccgc gtcgccgccg
     4021 gcgagggcgc cgaacgtgcc gtcctcgccc gggccgtcga cggggcgcca cgtgtacgtc
     4081 ccctggatgc cctgcaccag cggcgcggac gcggacgggc
//
LOCUS       DASUMX010000230         3516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1663886, whole genome shotgun sequence.
ACCESSION   DASUMX010000230 DASUMX010000000
VERSION     DASUMX010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3516
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1663886"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(153..1436)
                     /locus_tag="VFZ86_10820"
     CDS             complement(153..1436)
                     /locus_tag="VFZ86_10820"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX5642811.1"
                     /translation="MSGVDEASAGKSRLARTLAWSRTPIWPTVAVVVVLAFIPLVWRN
                     DYYISLFVLILLFAIMTQSWNITIGMCGIWNFGQLAIIALGGYATGLLVVKLGISPWL
                     ALLAGVVAGGVSAVIMVLPSIRLRGIYASMLTFAFAEVVRLLIIADSTGFTGGPFGLP
                     GVTGLFDWLSPSWSLRAYFWLTLVLTAAVMLFVHRMMNSPFGMGLVSLRESIRFSIGM
                     GINRRVAFIMATAVSGLIAGLAGGLYATFYHGITPSFMGLGPMGLLVIMVVVGGLGTV
                     RGPIIGAFVVTMLSELLRDTVMWRLVVQGALLLIVLAFWPAGLDGLFERGYDRVRAWM
                     NAGQDEGRPGVPAAAVAEGSTDQAAGRVAAAAVTDQVQRADRTDVAAAEPARPTKKVG
                     RFKAWLNADQPPKPGDTAGGPADDPVRRGADAGDA"
     gene            complement(1433..2320)
                     /locus_tag="VFZ86_10825"
     CDS             complement(1433..2320)
                     /locus_tag="VFZ86_10825"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX5642812.1"
                     /translation="METLLQAIISGLVFGAIYAIMTMGLTLVYGCLRTLNMAQGAFGM
                     VGGYAAWVLASRAGAPPLVGLVAGAVAAAGIGILTHFISVKPLVGRKDIDFEMTAYIS
                     TLVVAIMLSASIILLFGARNKAIDPLVEGRLQIFGTVSVSWQQVAVAVVALVCLGGLS
                     LLLNRSRQGLSIMAVAQQFDAARLMGVPAGRVYWVTMALAGMLGGIAGVLLAPLYFVS
                     PSAGDLPILKGLTVAIVAGLGSIRGTIYAAFIIGLIEALVATYVGSLWALPVLFSLIV
                     VFMIVRPYGLFGKPEEERL"
     gene            complement(2324..3034)
                     /locus_tag="VFZ86_10830"
     CDS             complement(2324..3034)
                     /locus_tag="VFZ86_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011749324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5642813.1"
                     /translation="MLEVTHLRAGYGAATVLEDVSISVDEGKFVVLTGPNGHGKTTLL
                     RVLSGLLPAGEGEVTFLGEPLNGKSAAAIVGRGLVHIPQGDLLFVDLTVEQNLFMGAY
                     LSAAWRSRRDRIKRVYELFPRLEERRGQQARTLSGGERRMLGIGRGLMSNARLLMVDE
                     PALGLAPVLVQEVYATLDRIARSEATVLLVEETLANARDIADYVYLMDTGRIVHEGTM
                     AEFGADVLSSTYLGNRGS"
     gene            complement(3027..>3516)
                     /locus_tag="VFZ86_10835"
     CDS             complement(3027..>3516)
                     /locus_tag="VFZ86_10835"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5642814.1"
                     /translation="YFGHRARGVVALSYDPETVERAEQAAEFVGLQDALADPGELLSA
                     FDRKRVMFAAALATRPDVLLLDEPAGGLSDQECAHLIDLIARVKAQGITVMIVEHVMS
                     VLMTVSDRVMMMHQGAKFFVGTPGEVQANPEVIKVYLGNVGAEAGVTLEELGALPEVR
                     DA"
BASE COUNT          590 a         1232 c         1211 g          483 t
ORIGIN      
        1 gcgggcacgt gctggaacga ccagcggttg aacggcggcg tctcccagtt ccagaacgtg
       61 acccgcttgt ccgggggcgg ggggctgccg cgcatgagcc ccagctcggc ggccgtcttg
      121 ggggtgtcgg cgatcacgcc ctcgacccct tcctaggcgt ctccggcgtc ggcgccacgc
      181 cgcaccgggt cgtccgccgg tcctcccgcc gtgtcgcccg gcttgggcgg ctggtcggcg
      241 ttgagccacg ccttgaacct gccgaccttc ttcgtgggcc gtgcgggctc cgccgcggcc
      301 acgtcggtcc ggtcggcgcg ctgcacctgg tccgtgaccg cggcggccgc gacccggccc
      361 gcggcctggt ccgtggaccc ctcggcgacg gcggcggccg gcaccccggg tcgtccctcg
      421 tcctggccgg cgttcatcca cgcccgcacc cggtcgtagc cgcgctcgaa caagccgtcg
      481 aggccggccg gccagaaggc cagcacgatc agcagcaggg ccccctgcac caccagccgc
      541 cacatgaccg tgtcgcgcag gagctcgctc agcatggtca cgacgaacgc cccgatgatc
      601 ggccctcgca ccgtgccgag gcccccgacg acgaccatga tcaccaggag ccccatgggg
      661 ccgaggccca tgaagctggg ggtgatgccg tggtagaagg tggcgtagag gccgcccgcc
      721 agtccggcga tcaggccgga aacggcggtg gccatgatga aggcgacgcg ccggttgatg
      781 cccatgccga tggagaagcg gatggattcg cgcaagctca cgagacccat gccgaacggc
      841 gagttcatca tgcggtgcac gaagagcatg accgccgcgg tcagcaccag cgtcagccag
      901 aagtacgcgc gcaggctcca ggagggcgag agccagtcga acagtccggt cactccgggc
      961 agcccgaacg gcccgccggt gaagccggtg ctgtcggcga tgatgagcag ccggaccacc
     1021 tccgcgaagg cgaacgtgag catcgaggcg tagatgccgc ggagacggat gctgggcagc
     1081 accatgatca ccgccgatac cccgcctgcg accacgcccg ccagcagcgc cagccacggc
     1141 gagatcccga gcttgacgac gaggagcccc gtcgcatagc cgccgagggc gatgatggcg
     1201 agctggccga agttccagat tccgcacatg cctatggtga tgttccagct ctgggtcatg
     1261 atcgcgaaca gcaggatgag gacgaacaga ctgatgtagt agtcgttgcg ccacaccaga
     1321 gggatgaacg ccagcaccac gacgaccgcg acggtcggcc agatcggcgt ccggctccag
     1381 gcgagcgtcc tggcgagcct gctcttgccg gctgaggcct cgtcgacgcc gctcacaggc
     1441 gctcctcctc cggtttgccg aacaggccgt aggggcgcac gatcatgaac acgacgatga
     1501 ggctgaagag cacgggcagc gcccaaagac tgccgacgta ggtggccacc agcgcctcga
     1561 tcaggccgat gatgaacgcc gcgtagatcg tgccgcggat gctgcccaga ccagcgacga
     1621 tggcgaccgt gaggcccttg aggatgggca ggtcgccggc cgacggcgag acgaagtaca
     1681 gcggcgccag caggactccg gcgatgccgc cgagcatgcc ggccagcgcc atggtgaccc
     1741 agtacacgcg tcccgccggc acgcccatga gccgcgccgc gtcaaactgc tgcgccacgg
     1801 ccatgatcga gagcccttgt cgcgaacggt tcagcagcag gctcaggccc ccgaggcaga
     1861 ccagggcgac cacggccacg gcgacctgct gccacgacac ggagaccgtg ccgaagatct
     1921 ggagccgccc ttcgaccagc gggtcgatcg ccttgttgcg ggccccgaac agcaggatga
     1981 tcgaggcgga gagcatgatc gccacgacga gcgtggagat gtacgcggtc atctcgaagt
     2041 cgatgtcctt gcggcccacg agcggcttga ccgagatgaa gtgcgtgagg atgccgatgc
     2101 cggccgccgc gacggcgccg gcgaccaggc cgaccagcgg cggcgcgccg gcgcgggagg
     2161 cgagcaccca ggcggcgtag ccccccacca tgccgaacgc tccctgcgcc atgttcagtg
     2221 tgcgcaagca cccgtagacg agggtgagcc ccatcgtcat gatcgcgtag atcgcgccga
     2281 agacgagccc gctgatgatc gcttgtagca aagtctccat gcatcagctc ccgcgattgc
     2341 cgaggtaggt ggacgagagg acgtcggcgc cgaactcggc catcgttccc tcgtggacga
     2401 tccgtcccgt gtccatgagg tacacgtagt cggcgatgtc ccgggcgttt gccagcgtct
     2461 cctccacgag cagcaccgtc gcctcgctgc gcgcgatcct gtccagcgtg gcgtagacct
     2521 cctgcacgag gacgggggcc aggcccaggg ccggctcgtc gaccatgagg agcctcgcgt
     2581 tgctcatgag accgcggccg atgccgagca tgcgccgctc accgcccgag agcgtgcgcg
     2641 cctgctgccc gcgccgctcc tcgaggcgag ggaagagctc gtagacgcgc ttgatgcggt
     2701 cacgccggct cctccaggcg gccgagaggt aggcgcccat gaacaggttc tgttccacgg
     2761 tgagatccac gaacaggagg tcgccctggg ggatgtggac cagaccgcgg cccacgatgg
     2821 cggccgccga cttgccgttg agcggctcgc cgaggaacgt gacctcgccc tcgccggccg
     2881 gcaacagccc gctgagcacg cgcaggagcg ttgtcttgcc gtgaccgttc ggccccgtga
     2941 ggaccacgaa cttgccctcg tcgacggaga tgctcacgtc ctcgagcacc gtcgccgcgc
     3001 cgtagccggc cctcaggtgg gtgacctcaa gcatcgcgga cctccggcag ggcgccgagc
     3061 tcctcgagcg tgacccctgc ctcggcgccg acgttcccca gatagacctt gatgacctcg
     3121 gggttggcct ggacctcccc gggggtaccg acgaagaact tggcgccctg gtgcatcatc
     3181 atgacgcggt cggagaccgt catgaggacg ctcatgacgt gctccacgat catcacggtg
     3241 atgccctgtg ccttgacgcg cgcgatcagg tcgatgaggt gcgcgcactc ctggtcactc
     3301 agcccgcccg ccggctcatc cagcaggagg acgtccggtc gcgtggccag ggcggcggcg
     3361 aacatgacgc gtttgcggtc gaacgccgag agcagctccc cgggatcggc gagcgcgtcc
     3421 tgcaggccca cgaactcggc ggcctgctcg gcgcgctcga ccgtctccgg gtcgtagctc
     3481 agggccacca cgccgcgcgc gcggtggccg aagtag
//
LOCUS       DASUMX010000231         2015 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_2096126, whole genome shotgun sequence.
ACCESSION   DASUMX010000231 DASUMX010000000
VERSION     DASUMX010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2096126"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..316)
                     /locus_tag="VFZ86_10840"
     CDS             complement(44..316)
                     /locus_tag="VFZ86_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006332270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2TM domain-containing protein"
                     /protein_id="HEX5642815.1"
                     /translation="MPTEEELREHAIKAIKKKRDFWNHVIAYCIVNVFLVVIWYWNGA
                     GYFWPGWVLAGWGIGLAFNAWDAYGRGSRPISEDEIQREMERQRRG"
     gene            486..1787
                     /gene="purB"
                     /locus_tag="VFZ86_10845"
     CDS             486..1787
                     /gene="purB"
                     /locus_tag="VFZ86_10845"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEX5642816.1"
                     /translation="MIERYTLPEMGAIWTDQARMQAWLDVEVAAVRAWNRLGKIPDDA
                     VREIEAKAAFDVARVNEIEATTNHDVIAFLTNVAEYVGDASKYVHYGMTSSDMLDTAL
                     ALQIKQAGRLLERDLVALGEVLKRRAFEFRDTVEVGRTHGIHAEPITFGMKLGIWAFE
                     VSRHLKRMRVATVAASVGKMSGAVGAYNNIDPRVEDLVCEELGIGREPISNQVVQRDR
                     HAEFLTVIALAGASIEKFATEVRHLQRSEVREAEEAFGKGQKGSSAMPHKRNPILSER
                     MAGCARVLRGNALVGMENIALWHERDISHSSAERIVLPDSTILLDYMLQKFTKLIDNL
                     VVDEERMLANLGQSYRLVFSGAVLLALVDKGMLREDAYRVVQDSAMHAWREGVDFGML
                     IKQNDEAMAVMTPGEVDAAMDPDQYRAGRDVIFARLEKLEF"
BASE COUNT          321 a          714 c          702 g          278 t
ORIGIN      
        1 gtcgccggcc gtcaccggcg cagcggcgcg gccgagcctc ggctcagccg cgccgctgcc
       61 gctccatctc ccgctggatc tcgtcctcgc tgatcggccg gctgccgcgc ccgtacgcgt
      121 cccacgcgtt gaaggcgagc ccgatgcccc agccggcgag cacccagccc ggccagaagt
      181 agccggcgcc gttccagtac cagatcacga cgaggaagac gttgacgatg cagtaggcga
      241 tgacgtggtt ccagaagtcg cgcttcttct tgatcgcctt gatcgcgtgc tcgcgcagtt
      301 cctcttccgt cggcatggct tcctcctgtg atcgcctgcg tcgccgccgc cgctctgctc
      361 gattgtgaac ccgcggcccg gcgcgcgcca gacgtcgcgg cgtgtgtgag agaatctcgg
      421 gcgacgacag gcggcgcccg gcgcgggccg ggccagccgt gacgcggcgg ccgcggaggg
      481 caccgatgat cgagcgctac acgctccctg agatgggcgc gatctggacg gaccaggcga
      541 ggatgcaggc ttggctcgac gtggaggtcg ccgccgtgcg ggcctggaac aggctcggca
      601 agatccccga cgacgccgtc cgcgagatcg aggccaaggc cgccttcgac gtcgcccgcg
      661 tcaacgagat cgaggccacg accaatcacg acgtcatcgc cttcctcacc aacgtggccg
      721 aatacgtcgg cgacgccagc aagtacgtcc actacggcat gaccagcagc gacatgctcg
      781 acaccgcgct ggcgctccag atcaagcagg ccggccggct gctcgagcgc gacctcgtcg
      841 ccctgggcga ggtgctcaag cggcgcgcgt tcgagttccg cgacacggtc gaggtcggcc
      901 gcacccacgg catccacgcc gagcccatca ccttcggcat gaagctcggc atctgggcgt
      961 tcgaggtcag ccgtcacctc aagcgcatgc gcgtggccac cgtcgccgcg tcggtgggca
     1021 agatgagcgg cgccgtcggc gcctacaaca acatcgaccc gcgcgtcgag gacctggtct
     1081 gcgaggagct cggcatcggc cgcgagccca tcagcaacca ggtggtgcag cgcgaccggc
     1141 acgccgagtt cctcaccgtc atcgccctcg ccggcgcgag catcgagaag ttcgccaccg
     1201 aggtgcggca cctgcagcgc tcggaggtcc gtgaggccga ggaggcgttc ggcaagggcc
     1261 agaagggctc ctcggccatg ccgcacaagc gcaaccccat cctcagcgag cgcatggccg
     1321 gctgcgcccg cgtgctgcgc ggcaacgcgc tggtcgggat ggagaacatc gccctgtggc
     1381 acgagcgcga catcagccac agctctgccg agcgcatcgt gctgcccgac agcaccatcc
     1441 tgctcgacta catgctgcag aagttcacga agctcatcga caacctcgtg gtggacgaag
     1501 agcgcatgct ggcgaacctg ggccagagct accgcctcgt gttcagcggg gcggtcctgc
     1561 tggccctcgt cgacaagggc atgctgcgcg aggacgccta ccgcgtcgtg caggactccg
     1621 ccatgcacgc ctggcgcgag ggcgtcgact tcgggatgct catcaagcag aacgacgagg
     1681 ccatggcggt gatgacccct ggggaggtcg acgccgccat ggacccggac cagtaccggg
     1741 ccgggcgcga cgtgatcttc gcccggctgg agaagctcga gttctgagcg ccgcggtcgc
     1801 cgatcgggtc atggcggggg gcgcggcggc cgatagactg ggcgtagtgg ccgcgcgagc
     1861 gcctgcggcc gagtcgacct cccgaggggg accggatggc cgagtgcccg agctgtaacg
     1921 ctccgctcaa ggacggcgac tggacctgcg gcgcgtgcgg agcgccggcc ggagtggccg
     1981 gcgcctcgac cggcggcccg gcctacggag cgccg
//
LOCUS       DASUMX010000232         6250 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_371343, whole genome shotgun sequence.
ACCESSION   DASUMX010000232 DASUMX010000000
VERSION     DASUMX010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 6250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6250
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371343"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /locus_tag="VFZ86_10850"
     CDS             <1..688
                     /locus_tag="VFZ86_10850"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5642817.1"
                     /translation="DAVMANSSVVARLLRDVVAPERLSVVLNGTTGLEEQVEPASDYL
                     PGEPLVLTVGQLIERKGTADLITALGKLRRDGRRVQLAIVGEGPLRQALEERAAAEGV
                     AGDVHFVGRMEHARVLAMMARAQLFALPSWDEAFGLVYTEAMAQGTPVLALQGGGPED
                     FIDHGVSGGWLIPARDPGAIAGVIARVLDDPAGAAAIGAAGRATALGLSWQRNARLTR
                     DVYERMLERR"
     gene            743..1381
                     /locus_tag="VFZ86_10855"
     CDS             743..1381
                     /locus_tag="VFZ86_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008147255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HEX5642818.1"
                     /translation="MSIERFDNVLVLAPHTDDGEFGCGGTMARLIENGVKVTYAAFST
                     AAKSVPEGFPKDVLKHEVRAATGILGIAETDLKVYDFEVRTFPTMRQDILEEMIVLQR
                     ELDPDCVLLPALIDLHQDHKTVAEEGLRAFKRTTVMAYEIPWNNLNFSQQAYVRLEER
                     HIQKKIEALGCYASQGHRNYLNEDYIRNVALTRGINIGCEYAEVFEVYRWIL"
     gene            1416..2459
                     /locus_tag="VFZ86_10860"
     CDS             1416..2459
                     /locus_tag="VFZ86_10860"
                     /inference="COORDINATES: protein motif:HMM:NF026968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX5642819.1"
                     /translation="MTLPALKIVLTAAGCPGASTLIGQLKANREREIHIHGVDMRPDA
                     VGRFMCDGFSLVPPGASPEYIPGLAAVVERERPDVLFVQSSHEIETVARHRDVFEALG
                     ARVLADGVRGIEIASDKAVMHAACGEIPVPQPELLRPASLEGFVAGARELGYPDVPVC
                     FKPPVAKGSRGFRILSAHVDRVHDLLYEKPINRYMTLDDFVDLFTGYEPFPELMLMEY
                     VDAPEITVDLFADKGEVVFHQTRTREEFLTGLAMAFKTIDRPELVELTRKVVRHLDLD
                     YYQSVQWIGGRLLEINPRVSTFVYQEDFNIPYLGIKYTLGEIGTDDVAEIQARVRTSR
                     RSVRYYDQVFWDD"
     gene            2518..3774
                     /locus_tag="VFZ86_10865"
     CDS             2518..3774
                     /locus_tag="VFZ86_10865"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX5642820.1"
                     /translation="MHILFLTHYFTPENNAPAARVHGLAREWARSGHRVTVLTCAPNV
                     PAGVVYEGYENKLYQEEWIDGIRTVRVWTWLAANKGRVRRGLNYVSYLAAAGAAGPLL
                     RPRADIVIATTPQFFAGWAGWPVARAHGAPFVLEVRDIWPDSIVAVGALGEGRITRTL
                     GKLERALYDSADHIVAVGDGYRMNMIRKGVGPSRISVITNGVDTHLFEPREPDQELRA
                     RLGFSPGTFVVTFAGTIGMASGLEVALGAARRLKEKGRDDIAFLLVGDGAVRAGLEET
                     ARAQGLANVVFTGLVPRTDLPAYLACSDACLVHFRKDELFGTILPSKFFEDAAMERPI
                     LLGFEGDARAMLHEADCGIAFEPSNPEELAAAAERLAAATPQERRRLGENGRRYVLEN
                     FDRRRLAHDYLEVLERVRADHKRGRR"
     gene            3872..4759
                     /locus_tag="VFZ86_10870"
     CDS             3872..4759
                     /locus_tag="VFZ86_10870"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HEX5642821.1"
                     /translation="MSTDDSTSIDVGSWFSTLARGWWVIVGLIILGIVVGAVVTLAQP
                     EEYTATASVYIGQTTDAGGNPMAGLNSNAKAAVQLLASQVVLEEAAERAGMDLSASRL
                     RRQTTVETPSSTVKTTTSVVNIVVISVTDTDAERAAAAANALAEVLLERLESGVDEKI
                     AVLEKQLADSQKAYAASLARSVAAQEALADIAKGGGSAADKAAAGSTWVALTQAAATE
                     QESLSKTIQQTELLLLSARQNEQPRMLHEAAVPDAPSGPSMTMNVAVGALAGLVIGVI
                     VVFARRGLAERRAARAAAA"
     gene            4867..>6250
                     /locus_tag="VFZ86_10875"
     CDS             4867..>6250
                     /locus_tag="VFZ86_10875"
                     /inference="COORDINATES: protein motif:HMM:NF027224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BNR-4 repeat-containing protein"
                     /protein_id="HEX5642822.1"
                     /translation="MPSASRVSVAALVVLVVALLVPAAAPAADHTGHIDLQDGLTLAE
                     QFGYRPDYRQHVVTFDAANVPSIRSRGASEDDTSFVQRLEDGAWVRHDMLEALREAYP
                     DFAGTLHAGGYGTDRVDWDVDGRAYTVLTIRLDDDGGGFRNVLMASTDGCVTWQVLEL
                     PFGDDTPLTDPHDWGNVTAEHDGGRPLEGPPLIAVWRQIGPWKGQWAALNHLQVIQPR
                     WVGGTLTLQPPVTVSSAALTLLQCSGGASFAVRSGDKTFFVYSTVVPRGSAATPTYAA
                     TYDHETNTVGTSTLAARSRPPNDLHCTPGICMDSTGTLHVVTGAHGYPFRYARSLAPL
                     STAAWTRQVNVLMSGYRARGTDTDGLGKQTYLSMVCGPDDVLHVVSRQARRNVDEHFR
                     GSGYSALVHQSLQPGSTAWSTPHLVVAPPLPGYSQYYQKLTVDRLGRLFVSCSYFSLR
                     DPPATRAYRRF"
BASE COUNT          953 a         2330 c         2117 g          850 t
ORIGIN      
        1 cgacgcggtg atggcgaact ccagcgtcgt cgcccgcctg ctccgcgacg tggtcgcacc
       61 ggagaggctg tcggtcgtgc tcaacggcac caccggtctc gaagagcagg tcgagccggc
      121 gtccgactac ctccccggcg aaccgctcgt gctcaccgtg gggcagctga tcgaacgcaa
      181 gggcacggcc gacctcatca ccgccctggg caagctgcgc cgcgacggcc ggcgcgtgca
      241 gctcgcgatc gtcggcgagg ggccgctgcg ccaggcgctc gaggagcgcg ccgcggccga
      301 gggcgtggcc ggcgacgtcc acttcgtcgg ccgtatggag cacgcccggg tgctcgcgat
      361 gatggcgcgc gcgcagctct tcgccctgcc gagctgggac gaggccttcg ggctcgtcta
      421 caccgaggcc atggcccagg gcacgcccgt gctcgccctc caggggggag gccccgagga
      481 cttcatcgac cacggcgtga gcggcggctg gctgatcccc gcgcgtgatc ccggcgccat
      541 cgcgggagtc atcgcccggg tgctcgacga cccggccggg gcggcggcga tcggcgctgc
      601 gggtcgcgcc accgccctcg gcctcagctg gcagcgcaac gcccgcttga cgcgcgacgt
      661 ctacgaacgc atgctcgagc gccggtgagg cggtacggga cgatttggta gacttctacg
      721 gttcgctgat gaaaggccct tcgtgagcat cgaacgcttc gacaacgtgc tcgtgctggc
      781 gccgcacacc gacgacggtg agttcggctg cggcggcacc atggcgcgcc tcatcgagaa
      841 cggcgtcaag gtcacctacg ccgccttcag caccgccgcc aagagcgtcc ccgagggctt
      901 tcccaaggat gtgctcaagc acgaggtccg cgctgcgacc ggcatcctcg gcatcgccga
      961 gaccgacctc aaggtgtacg acttcgaggt gcgcaccttc ccgaccatgc gccaggacat
     1021 cctcgaggag atgatcgtcc tgcagcgcga gctcgacccg gactgcgtgc tgctgccggc
     1081 gctgatcgac ctgcaccagg accacaagac cgtggccgag gaaggcctgc gcgccttcaa
     1141 gcgcaccacc gtcatggcct acgagatccc gtggaacaac ctcaacttct cgcagcaggc
     1201 ctacgtacgg ctcgaggagc gccacatcca gaagaagatc gaggccctgg ggtgctacgc
     1261 cagccagggc caccgcaact acctgaacga ggactacatc cgcaacgtcg ccctcactcg
     1321 cggcatcaac atcggctgcg agtacgccga ggtgttcgag gtctaccgct ggatcctcta
     1381 gcgccccacc cagccgcgac cagtccggag tccccatgac gctgccggcg ctcaagatcg
     1441 tcctcaccgc cgccggctgc cccggcgcga gcacgctgat cggacagctc aaggccaacc
     1501 gcgagcgcga gatccacatc cacggcgtcg acatgcggcc ggacgccgtc ggcaggttca
     1561 tgtgcgacgg cttcagtctc gtgccgccgg gggcctcgcc cgagtacatc ccggggctcg
     1621 ccgccgtcgt cgagcgcgag cgcccggacg tgctcttcgt ccagtcctcg cacgagatcg
     1681 agacggtggc ccgtcaccgc gacgtgttcg aggcgctcgg ggcgcgcgtg ctcgccgacg
     1741 gcgtgcgcgg catcgagatc gccagcgaca aagcggtcat gcacgccgcc tgcggcgaga
     1801 tccccgtgcc gcagccggag ctgctccggc cggcgagcct cgaggggttc gtcgccggcg
     1861 cccgggagct cggctacccg gacgttcccg tgtgcttcaa gccaccggtc gccaagggtt
     1921 cgcgcggctt ccgcatcctc tccgcgcacg tcgaccgcgt ccacgacctc ctctacgaga
     1981 agccgatcaa ccggtacatg acgctcgacg acttcgtcga cctcttcacc ggctacgagc
     2041 cgttccccga gctcatgctg atggagtacg tcgacgcgcc cgagatcacc gtcgacctct
     2101 tcgccgacaa gggggaggtg gtcttccacc agacgcgcac ccgcgaggag ttcctcaccg
     2161 gccttgcgat ggcgttcaag accatcgacc gcccggagct ggtggagctc acgcgcaagg
     2221 tcgtccggca cctggacctg gactactacc agagcgtgca gtggatcggc ggccggctgc
     2281 tcgagatcaa cccgcgcgtc agcacgttcg tgtaccagga ggacttcaac atcccgtacc
     2341 tcgggatcaa gtacacgctc ggcgagatcg gcacggacga cgtggcggag atccaggcgc
     2401 gggtgcgcac gagccgcagg tcggtgcgct actacgacca ggtgttctgg gacgactgag
     2461 agagcggccg gccggcgccg gccggccccg gcccgccgag gaggaggctc accgggcgtg
     2521 cacatcctgt tcctgacgca ctacttcacc cccgagaaca acgcccccgc cgcgcgcgtg
     2581 cacggtctcg cgagggagtg ggcgcgcagc gggcaccgtg tcacggtgct gacctgcgcg
     2641 cccaacgtgc ccgccggggt cgtgtacgag ggctacgaga acaagctgta ccaggaggag
     2701 tggatcgacg gcatccgcac ggtccgcgtc tggacctggc tcgcggccaa caagggccgc
     2761 gtgcgccgcg gcctcaacta cgtctcgtac ctggccgcgg cgggcgcggc cggcccgctg
     2821 ctgcggccgc gcgccgacat cgtcatcgcc accactccgc agttcttcgc cggctgggcc
     2881 ggctggccgg tcgcgcgcgc gcacggcgcg ccgttcgtgc tcgaggtccg cgacatctgg
     2941 cccgactcca tcgtcgccgt gggcgcgctc ggagagggtc gcatcacccg gacactcgga
     3001 aagctggagc gcgcgctcta cgacagcgcg gaccacatcg tcgccgtcgg cgacggctac
     3061 cgcatgaaca tgatccgcaa gggcgtcggc ccttcccgca tctcggtcat caccaacggc
     3121 gtcgacaccc acctgttcga gccgcgcgag cccgaccagg agctccgcgc gcggctgggc
     3181 ttctcgcccg ggaccttcgt cgtcaccttc gccggcacca tcggcatggc cagcggcctc
     3241 gaggtcgcgc tcggcgcggc ccgcaggctc aaggagaagg gccgcgacga catcgccttc
     3301 ctgctcgtcg gcgacggcgc cgtgcgcgcc gggctggagg agaccgcgcg cgcgcagggc
     3361 ctcgccaacg tggtcttcac cggcctcgtg ccgcgcaccg accttccggc ctacctcgcc
     3421 tgcagcgacg cctgcctcgt gcacttccgc aaggacgagc tgttcgggac gatcctgccg
     3481 agcaagttct tcgaggacgc cgccatggag cggccgatcc tgctcgggtt cgagggcgac
     3541 gcgcgggcga tgctgcacga ggcggactgc ggcatcgcct tcgagccgtc caaccccgag
     3601 gagctggcgg cggcggccga gcgcctcgcc gccgccacgc cgcaggagcg gcggcggctc
     3661 ggcgagaacg gccgccgcta cgtgctcgag aacttcgacc gccgcaggct cgcgcacgac
     3721 tacctcgaag tcctcgagcg cgtgcgcgcc gaccacaagc gcggccgccg gtgagcgccc
     3781 gggccggctg cgccgccggg cctgcgccgc gacccgctcc atccgtctgc ggtacactgg
     3841 cgcgaagcgg cagccgcccc cggaggagcg catgagcact gacgacagca ccagcatcga
     3901 cgtcgggtcc tggttctcca cgctcgcccg cggctggtgg gtgatcgtcg ggctcatcat
     3961 cctgggcatc gtcgtcggcg ccgtcgtcac cttggcgcag cccgaggagt acaccgccac
     4021 cgcatccgtg tacatcggac agaccaccga cgccggcggc aaccccatgg ccgggctcaa
     4081 ctccaacgcc aaggccgccg tccagctgct cgccagccag gtggtgctcg aggaggccgc
     4141 cgagcgcgcc ggcatggacc tctccgcgag ccggctgcgc agacagacca cggtggagac
     4201 accgtcgtcg acggtcaaga cgacgacgtc ggtggtcaac atcgtcgtca tcagcgtcac
     4261 cgacacggac gcggaacgcg cggccgcggc cgccaacgcg ctcgccgagg tgctgctcga
     4321 acgcctggag agcggcgtgg acgagaagat cgcggtactc gagaagcagc tcgcggacag
     4381 ccagaaggcc tacgccgcgt cactggcacg cagcgtcgcc gcccaagagg cgctcgccga
     4441 catcgccaag ggcggaggct cggccgccga caaggcggcg gcggggtcca cctgggtggc
     4501 gctcacgcag gcggcggcga ccgagcagga gtcactctcc aagacgatcc agcagacgga
     4561 gctcctgttg ctgtcggccc ggcagaacga gcagccgcgg atgctgcacg aggcggcggt
     4621 ccccgacgcg ccgagcgggc cgagcatgac gatgaacgtc gccgtcggcg cactcgccgg
     4681 attggtgatc ggggtcatcg tcgtcttcgc gcgccggggc ctcgcggagc ggcgcgccgc
     4741 ccgcgccgcc gcggcctgac cgccagttct ccgcaacccg gctcgcgacg agccacggcc
     4801 ggaggacgac gcgggacgga tggtagactg gcgcggtcat ggggtccatc acaggaggtg
     4861 gcagcagtgc cctccgcatc ccgcgtgtcc gtcgccgcgc tcgtcgtgct cgtcgtcgcc
     4921 ctgctggtcc cggccgcggc gccggccgcc gaccacacgg ggcacatcga ccttcaggac
     4981 gggcttaccc tggcggagca gttcgggtac cgtcccgact accgccagca cgtcgtgacc
     5041 ttcgacgcag ccaacgtgcc gagcatccgc agccgtggcg cgagcgagga cgacacttcc
     5101 ttcgtgcagc gcctcgagga cggcgcctgg gtgcggcacg acatgctcga ggctctgcgc
     5161 gaggcgtacc ccgacttcgc cggcacgttg cacgcagggg ggtacggaac cgaccgcgtc
     5221 gactgggacg tggacgggcg cgcctacacc gtgctcacca tccgcctcga cgacgacggc
     5281 ggcggtttcc gcaacgtgct catggcctcg acggacggct gcgtcacctg gcaggtgctc
     5341 gagctgccgt tcggcgacga cacccccctg accgacccgc acgactgggg caacgtcacc
     5401 gcggagcacg acggcggccg gccgctcgag gggccgccgc tcatcgccgt ctggcggcag
     5461 atcggcccct ggaagggaca gtgggccgcg ctcaaccatc tccaggtcat ccagccccgc
     5521 tgggtcgggg ggacgctcac cctgcaaccg ccggtgacgg tgagctcggc ggcgctcacg
     5581 ctgctccagt gctccggcgg cgcctcgttc gccgtcaggt cgggggacaa gaccttcttc
     5641 gtctacagca ccgtcgtgcc gcgcggcagc gcggcgacgc ccacctacgc cgccacctac
     5701 gaccacgaga cgaacacggt ggggacgagc accctggcgg ccaggtcgag gccgcccaac
     5761 gatctccact gcacccccgg catctgcatg gacagcaccg ggacgctgca cgtggtcacc
     5821 ggcgcgcacg gctatccctt ccgctacgcg cgctccctgg cgccgctctc gaccgccgcc
     5881 tggacgcgcc aggtcaacgt gctcatgtca ggctaccgcg ctcggggcac cgacacggac
     5941 ggcttgggca agcagaccta cctctcgatg gtctgcggac ccgacgacgt gctgcacgtc
     6001 gtcagccgcc aggccaggcg caacgtcgac gagcacttcc gcggcagcgg gtacagcgcc
     6061 ctggtgcatc agtcgctgca gcccggctcg accgcctgga gcaccccgca cctcgtggtc
     6121 gcgccgccgc tccccgggta ctcgcagtac taccagaagc tcaccgtgga tcgcctcggg
     6181 cggctcttcg tctcctgcag ctacttctcg ctgcgcgacc cgccggcgac gcgggcctac
     6241 cggcggttcc
//
LOCUS       DASUMX010000233         8654 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_659990, whole genome shotgun sequence.
ACCESSION   DASUMX010000233 DASUMX010000000
VERSION     DASUMX010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 8654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8654
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_659990"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /gene="thiT"
                     /locus_tag="VFZ86_10880"
     CDS             complement(<1..570)
                     /gene="thiT"
                     /locus_tag="VFZ86_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012446766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HEX5642823.1"
                     /translation="MSTPVVHSPGAPDVRARLNLNPAHWGVRAIAEIGVAVALAAALS
                     WIAQAFPLRMPQGGSFGLEMLPILFIALRRGVVPGVVAGALFGLLQLAGVAGTPYIYH
                     PLQAALDYPLAFAALGLAGLVPIGELHGARGVARLTAAVAAGTGLRFLCHFLSGLIFF
                     AEYAPAWEAPWLYSITYNLLYLLPSAVVTA"
     gene            1187..2032
                     /locus_tag="VFZ86_10885"
     CDS             1187..2032
                     /locus_tag="VFZ86_10885"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5642824.1"
                     /translation="MESFLDRLLADQGAAARRSAPRPAPPAASASAPTVATADGALPA
                     VPLGLEAPRIAIYDTLTSPPRVIAVEEEDLPALIASLAEKTYHYCREQGGQVPYSVLQ
                     EIIENLLHACFRDVVITILDNGQTIRISDHGPGVGDKDRAFLPGFTTATAYQRQIIRG
                     VGSGLPLARESLQFLRGILTAEDNLGGGAVFTIKMPSATETPPAAGGLPKVKLTTRQT
                     KVLVLLMELGSAGPTAIAKELGFSAATVYRELVVLQDMGLIHSLGDGKRALHEEGLRL
                     LESIL"
     gene            2115..3503
                     /gene="dnaA"
                     /locus_tag="VFZ86_10890"
     CDS             2115..3503
                     /gene="dnaA"
                     /locus_tag="VFZ86_10890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEX5642825.1"
                     /translation="MWEEAKKHLRGRIADAAFQFWFDRTVPLGLDDGAFVIGVPNDFA
                     REWIEKRLVGQVAEALEQVLGDAVEVKIVVDARAAALPDKPAAETPVETPVETAAPKV
                     KRGDRRKKPRATRAAGDLNPRYVFDRFVTGPNNEYATAVARSVAESPASAYNPVFIYA
                     DTGLGKTHLTQAIAHAALEQHPELQVRYVTCERFTDDFINAVTDKGRIEGFKQTYRDN
                     DVLIIDDVQFLAEKQQTQVEFFHTFNALYEAGKQIVITSDRPPRELEVLEERLRSRFG
                     QGVVVDISRPDLETRIAILRKQVKWEGFEIAEPEALELIAQKVTTNIRELYGALTRAV
                     SFASIRGVDVTVETTKEALKDIVPRAYQHPVTIEMVQNEVARQFGLHVNDLRGNRRTQ
                     DVAYARHIAMYLSRELTEASLPQIGTRFGGRHHSTVIHAVDKVERQLKDGHDPQLQDL
                     VALIGARLKTAH"
     gene            3763..4875
                     /gene="dnaN"
                     /locus_tag="VFZ86_10895"
     CDS             3763..4875
                     /gene="dnaN"
                     /locus_tag="VFZ86_10895"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEX5642826.1"
                     /translation="MKFTCDRSALVDKLGVLSRGVSTRSALPVLSGILLQAGEGRLDL
                     FSTDMELSIKANLSTPVEREGEIVVPARLFSDVVRNLPDEEVVIEAGEAAIKVSAGRA
                     AFSLNAWAAADFPQTSTFDMTGAFTVGRDPFVETLNKVGRAASRDETRPILTGVLMTI
                     MGDTLKMVATDSYRLAVKETKLGHALETEVQAIVPVKALGEVTRLAAALGPGDIEVAV
                     GENQAVFKLSDPAGDVWIASRLIEGQFPNYRQLLPDTFEHQVTIDRGQLMATARRVSL
                     LAQKNAPLRMSFADNRLTMKALTQDVGQAEEALDVEFSGEPFEIGFNPGYLIEGVDAV
                     DDEAAVLRFTSPLRPGLVSGPGDDFTYLIMPIRLSS"
     gene            4906..5121
                     /locus_tag="VFZ86_10900"
     CDS             4906..5121
                     /locus_tag="VFZ86_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019854082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEX5642827.1"
                     /translation="MDDVAVSGPLPLGAFLKLAGVAATGGHAKIIVQEGDVSVNGEVE
                     RRRGRTLQPGDVVAVSGREYRVWSSPA"
     gene            5103..6395
                     /gene="recF"
                     /locus_tag="VFZ86_10905"
     CDS             5103..6395
                     /gene="recF"
                     /locus_tag="VFZ86_10905"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HEX5642828.1"
                     /translation="MVVSGLRLVNFRSYCDATVRFGPGLNVVVGENATGKTNLLEAAW
                     FVLRGSSPRTRRDEKLITWNERFARVELTLESEEDAGSHQVDVGFAPGQGKRMRWGGL
                     EITSLDDLRGRSQVFIFVPESLLLVKGSPARRRAHLDAFAAPLDPQYAGAARALQGVL
                     RQRNAQLAAVKNGADPAALDPWDQQFARAATEFGRRRRDLVDELTAGFAETAAALAPA
                     GERFTLRLIAQLESVDYDEASLLDELRERRPGEVSRGLSLFGPHRDDLKLLEVGQTTG
                     RSPASRELGAAAQAAPSASAIPTVSGGPACGPVDLPRGGRDLRLFGSQGEQRAAVLAL
                     LLAEQRLAAARTGQQGPLFLDDVMSELDDPRRRLLVRMLTTAGQVIITTTNRHYFTDD
                     ELARATVIELPLDGSLAAGQAVPPPDDAPPAQPALDAL"
     gene            6392..7042
                     /locus_tag="VFZ86_10910"
     CDS             6392..7042
                     /locus_tag="VFZ86_10910"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HEX5642829.1"
                     /translation="MSGERRGARRGPSEGAGGRRPTGGRRDDLPRRLGDIITPALGRL
                     AGGDEARAYSAWARAVGDPVSGGTRPKAFRRGQLTIECASSVWANELTYLGPQILRRM
                     DEVAPGHPVKRFRFVVGRLPAPPAGVGDAVDGATTAHRDHPPEGQPIELPTQESGTPA
                     AKEKRRRQQPAPDAQEAAQAQVEGVHDARLRVAIEASLRRSSEEPSGASGRGTPSG"
     gene            7179..>8654
                     /locus_tag="VFZ86_10915"
     CDS             7179..>8654
                     /locus_tag="VFZ86_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HEX5642830.1"
                     /translation="MKKSNYDAQDIQVLEGLEPVRERPGMYIGSTGPRGLHHLVYEVI
                     DNSVDEALAGYCTAIVATINPDNSITVADNGRGIPVGIMEKYNLPAATIVLTMLHAGG
                     KFGGAGYKVSGGLHGVGVSVVNALSEWLELDIYVDGYHWNQRFERGNPVTELVKREKL
                     DKDGPTGTTVSFLADPDIFEEVDYDYRVLAQRLRETAFLTKGLKISLNDERADGESVT
                     FQYEGGIRDFVAYINREKDAIHRSIIYLENETPDGSVEIAMQWNSSYNESVFTFANNI
                     NTTEGGSHLTGFRSALTRTLNDYARQKNLLKEKEDNLTGEDMREGLTALVSVKLKNPQ
                     FEGQTKTKLGNTEIATLVATSVNEKLAEFLEENPSEGRQIVEKAINAARARSAARKAR
                     DLTRRKGLLEGSTLPGKLADCSIKDAALSEIYLVEGDSAGGSAKQARDRSFQAILPLR
                     GKIINVEKARIDKMLQNQEILTMISAIGAGIGEEFDVEQARY"
BASE COUNT         1509 a         3029 c         2852 g         1264 t
ORIGIN      
        1 cgccgtcacg acggccgacg gcagcaggta cagcaggttg taggtgatcg agtacaacca
       61 cggcgcttcc cacgcgggcg cgtactcggc gaagaagatc aggccggaga ggaagtgaca
      121 cagaaagcgc aggccggtgc cggccgccac ggcggcggtg agccgagcca ctcctcgcgc
      181 cccgtggagc tcgccgatcg ggacgagccc ggcgagcccc agagcggcga acgccagcgg
      241 atagtcgagc gccgcttgga gagggtgata gatgtacggg gtgccggcga cgccggcgag
      301 ttgcagcagt ccgaagaggg cgccggcgac gactccgggc accaccccgc gccgcagggc
      361 gatgaacagg atgggcagca tctcgaggcc gaacgatcca ccctgcggca tgcgcagggg
      421 aaaggcttgg gcgatccagc tcaaagccgc ggccagcgcg acggcgacac cgatctcggc
      481 gatggcgcgc acgccccagt gggcgggatt gaggttgagg cgcgcgcgca cgtcgggcgc
      541 gcctggtgaa tggacgacgg gtgtgctcac gttgacatct ctcccttcgc tggcattacc
      601 cagcagcgcc acgggcgccg ccgccccgcg ggggcggtca ggttcgatgc gtcggcgcca
      661 ggaagcgcca tctcagctcc tcacgcggga gctccgccgg gacatcgaca gtgtaccgca
      721 gcggatgggg cgggcgggcc acgagcgttt catcccgctg ctcctccgtt cacagccccc
      781 tgtggatagt gtggataact cgcttttgtc atgctctcag gctcacgatt gcggtggata
      841 aggcgttgac gcagaaatgc tgccttgaca cgacgctcac acggtactca tccgcagaaa
      901 tcacgccttt gcagggaagt acgctctatt ctgctccgca ggaacggggt tgacagatcg
      961 cgtaccgacg ggtttggacc acccagagcg gcatgccacc gtccaccccc cctgaaaccg
     1021 gggtttccac aacggccgca aattgggcat tcccgggcgg ccccgccggg tcgctttcca
     1081 caaatcgtga acaggctgtg gacaacttcc atctttcacc tgcaaaacca cgtttcatca
     1141 tgtgccatgg aggcttttcg gaggccctgc tatactcggc gcccccatgg agtcgttcct
     1201 cgacaggctg ctggccgatc agggggccgc cgcccggcgt tccgcgccgc ggcccgcccc
     1261 ccctgcggcc tcggcgagcg cccccactgt cgccactgcc gacggcgcgc ttcccgcagt
     1321 cccgctcggc ctcgaggccc cccgcatcgc gatctacgac accctgacga gtccaccgcg
     1381 cgtgatcgcc gtcgaggagg aggacctccc cgccctcatc gcgtctctgg cggagaagac
     1441 gtaccactac tgccgtgagc aaggggggca ggtgccgtat tcggtcctcc aggagatcat
     1501 cgagaacctc ctgcacgcct gcttccgcga cgtcgtgatc accatcctcg acaacggtca
     1561 gacgatccgc atctccgacc acggccccgg ggtgggggac aaagaccgcg cgttcctccc
     1621 cggcttcacc accgcgaccg cctaccagcg gcagatcatc cgcggcgtgg gctcaggcct
     1681 gccccttgcc cgtgagtctc ttcagttcct gcgcggcatc ctgaccgccg aggacaatct
     1741 cggcggcggc gccgtgttca ccatcaagat gccgtccgcg acggagacgc cgccggccgc
     1801 cggcgggctc cccaaggtca agctcacgac ccgccagacg aaggtgctgg tgctgctcat
     1861 ggagctcggc tctgcgggcc ccaccgccat cgccaaagag ctcggattct cggcggccac
     1921 ggtctatcga gaactcgttg tactgcaaga catggggttg atacactcgc tcggcgacgg
     1981 caagcgagcc ttgcacgagg agggtctgcg cctcctcgag agcatcctct gaccgacgtt
     2041 catgaacccc cggcgggccg ccgcatcccc ggggtcgacc gggtggggca gcgacggacg
     2101 acgagctcac acgtatctgg gaagaggcga agaagcacct ccgcggacgc atcgccgacg
     2161 ccgccttcca gttctggttc gacaggaccg tcccgctggg tctcgacgac ggcgcgttcg
     2221 tcatcggcgt tcccaacgac ttcgcccgcg agtggatcga gaagcgcctc gtcggccagg
     2281 tcgccgaggc gctcgagcag gtgctcggcg acgccgtcga ggtcaagatc gtcgtggacg
     2341 cgcgagcggc ggcgctgccg gacaagcccg ccgccgagac acccgtcgag acgccagtcg
     2401 agacggcggc gccgaaggtc aagcgggggg accgccgcaa gaagcctcgc gcgacccgtg
     2461 ccgccggcga cctcaacccg cggtacgtct tcgatcgctt cgtcaccggt cccaacaacg
     2521 agtacgcgac cgcggtagcg cgcagcgtcg cggagtcgcc ggcgagcgcc tacaacccgg
     2581 tcttcatcta cgccgacacc gggctgggca agacgcacct cacacaggcg atcgcccacg
     2641 cggccctcga gcagcacccg gaactgcagg tccgctacgt cacctgcgag cggttcaccg
     2701 acgacttcat caacgccgtc accgacaagg ggcgcatcga gggcttcaag cagacctacc
     2761 gcgacaacga cgtgctcatc atcgacgacg tgcagttcct ggcggagaag cagcagacac
     2821 aggtggagtt cttccacacg ttcaacgcgc tgtacgaggc cggcaagcag atcgtgatca
     2881 ccagcgaccg gccgccgcgc gagctggaag tgctcgagga gcgcctgcgc tcgcgcttcg
     2941 gtcagggcgt ggtggtcgac atcagccggc cggacctcga gacccgcatc gccatcctgc
     3001 gcaagcaggt caagtgggag ggcttcgaga tcgccgagcc cgaggctctc gagttgatcg
     3061 cgcagaaggt cacgaccaac atccgtgagc tctacggagc cctgacgaga gccgtcagct
     3121 tcgcctccat ccgtggggtc gacgtcaccg tcgagaccac caaggaggcg ctcaaggaca
     3181 tcgtccccag ggcgtaccag catccggtca ccatcgagat ggtccagaac gaggtggccc
     3241 ggcagttcgg cctccacgtc aacgacctgc gcggcaaccg gcgcacccag gacgtcgcct
     3301 acgcacggca catcgcgatg tacctctcca gggaactcac ggaggcctcg ctcccacaga
     3361 tcggcaccag gttcggcggc cggcaccaca gcaccgtcat ccatgcggtc gacaaagtgg
     3421 agcggcaact caaggatggc cacgatccac agttacagga cctcgtggct ctcatcggcg
     3481 cccgcctcaa gaccgctcac tgagacctct ccacaccccc tgtggacgac tccctcgtca
     3541 cctgtggacg atccccacgg gagtgttgaa atcggctatg cttcgctcac catcgacgcc
     3601 gccgcgggga ggagcgcaac gttctgcaca caccgtccac accactgtgc agaggctccc
     3661 gccctcactg tgcagcgaca agaccacctc tccacatcgt caacaggcgc tatggtgacg
     3721 gcgaggagta tctacttctc tttcttttca taaggagccg ggatgaagtt cacgtgtgat
     3781 cgctctgcgc tggtcgacaa gctcggcgtc ctctcgcgcg gcgtctccac gcgcagcgcc
     3841 ctccccgtgc tgtccgggat cctgctgcag gccggtgagg gtcgcctcga cctgttctcc
     3901 accgacatgg aactctcgat caaagccaat ctgtccaccc cggtcgagcg ggagggcgag
     3961 atcgtcgtcc cggcgcgcct cttcagcgac gtcgtccgca acctccccga cgaggaggtc
     4021 gtgatcgagg ccggcgaagc ggcgatcaag gtcagcgccg gccgggccgc tttctcgctc
     4081 aacgcctggg cggcggccga cttcccgcag acgtccacgt tcgacatgac cggcgccttc
     4141 accgtgggcc gggatccgtt cgtcgagacg ctcaacaagg tgggccgcgc tgcttcgcgt
     4201 gacgagacgc ggcccatcct caccggcgtc ctcatgacga tcatgggcga cacgctgaag
     4261 atggtggcga ccgacagcta ccggctggcg gtcaaggaga ccaagctcgg tcacgcgctc
     4321 gagaccgagg tccaggccat cgtcccggtg aaggcgctcg gagaggtcac gcgtctcgcc
     4381 gccgccctcg gcccgggcga catcgaagtc gcggtcggcg agaaccaggc tgtgttcaag
     4441 ctcagcgacc cggccggcga cgtgtggatc gcctctcgtc tcatcgaggg ccagttcccc
     4501 aactacaggc aactgctccc ggacaccttc gagcatcaag tgaccatcga tcgcggccag
     4561 ctcatggcga ccgcccggcg cgtcagcctc ctggcgcaga agaacgcgcc gctgcgcatg
     4621 tccttcgccg acaaccgtct cacgatgaag gcgctcaccc aggatgtggg gcaggccgag
     4681 gaggccctgg acgtcgagtt ctcgggagaa ccgttcgaga tcggcttcaa tcccgggtat
     4741 ctcatcgagg gtgtcgacgc cgtcgacgat gaggcggccg tgctgcgctt caccagcccg
     4801 ctgcgcccgg gcctcgtcag cggccccggc gacgacttca cgtatctcat catgccgatc
     4861 cggctgagca gctgaccgct gccgcctgac ccggccaccg tcgacgtgga cgacgtcgcc
     4921 gtgagcggcc cgctgccgct gggagcgttc ctcaagctcg ccggcgtcgc ggcgaccggc
     4981 ggtcacgcca agatcatcgt ccaggagggt gacgtcagtg tgaacggcga ggtcgagagg
     5041 cggcgcggac ggacgctcca gccaggcgac gtcgtcgccg tgagcggcag ggagtaccgc
     5101 gtgtggtcgt ctccggcctg agactggtca acttccgctc gtactgcgac gccacggtcc
     5161 ggttcgggcc tggtctcaat gtggtggtcg gtgagaacgc gaccggcaag acgaacctcc
     5221 tcgaggccgc ttggttcgtg ctgcgtggga gctcgccgcg gacgcgccgc gacgagaagc
     5281 tcatcacctg gaacgagcgg ttcgcgcgcg tggagctgac gctggagagt gaagaggacg
     5341 cagggtcgca tcaggtcgac gtgggattcg cccccggcca gggcaagcgc atgcgctggg
     5401 gagggctcga gatcacctcg ctcgacgacc tgcgcgggcg cagccaggtg ttcatcttcg
     5461 tgcccgagag cctgctgctg gtcaagggca gcccggcgag gcggcgtgct catctggacg
     5521 ccttcgccgc gccgctcgat ccgcagtacg cgggggcggc gcgcgcgctg cagggcgtcc
     5581 tccgccagcg caatgcccaa ctggcggccg tgaagaacgg cgccgacccg gcagcgctcg
     5641 atccctggga ccagcagttc gcgcgggcgg cgacggagtt cggacgccgc cgccgcgacc
     5701 tcgtcgacga gctcacggcg gggttcgcgg agaccgccgc cgccctcgcg ccggccggcg
     5761 agcgcttcac cctcagattg atcgcgcagc tggagagcgt cgactacgac gaggcgtcgc
     5821 tgctcgacga gctgagggag cgccggccgg gagaggtgag ccggggactc tctctgttcg
     5881 ggccccaccg cgacgacctg aagctcctcg aggtggggca gacgacgggc cggtcgccgg
     5941 cgtcgcggga gctcggggcg gcggcacaag ccgcgccgtc agcctccgcg atacccacgg
     6001 tgagcggcgg cccggcctgt ggtcctgtcg atcttccccg gggcggcagg gacctgcggc
     6061 tcttcggctc ccagggcgag cagcgagccg ccgtcctcgc cctgctgctg gcggagcaac
     6121 gccttgccgc cgcgcgtacc ggccagcagg ggcccctctt cctcgacgat gtcatgagcg
     6181 agctcgatga cccccgccgc cgcctgctcg tgcgcatgct gacgacggcg gggcaggtga
     6241 tcatcacgac gaccaaccgg cactacttca ccgatgacga gctcgcacgg gcaacggtca
     6301 tcgaactccc gctggatggc tcgctcgccg cgggccaagc cgtgccgcct ccggacgacg
     6361 caccgccggc gcagccggcg ctggacgcct tgtgagcggc gagcggcgcg gcgcccggcg
     6421 gggcccttcc gaaggcgccg gcggtcgcag gccgaccggc ggccgccggg acgacctgcc
     6481 acgcaggctc ggagacatca tcacgccggc gctcggcagg ctggctgggg gagacgaggc
     6541 gcgtgcgtat tccgcatggg cgcgcgccgt cggggatccg gtgagcggcg gtacgcgccc
     6601 gaaggcgttc cgccgcgggc agctcaccat cgaatgcgcg tcctcggtgt gggccaacga
     6661 gttgacctat ctcggcccgc agatcctgcg gcgcatggac gaggtggcgc cgggtcatcc
     6721 ggtcaagcgg ttccgcttcg tcgtcggccg cctgccggcg cctcccgcag gcgttgggga
     6781 cgcggtcgat ggcgcgacga ccgcgcatcg cgatcaccct ccagaaggcc agcccataga
     6841 gctgccgacg caggagtcgg ggaccccggc ggcgaaggaa aagcgtcggc gccaacagcc
     6901 cgcgcccgac gcgcaggagg cggcgcaggc ccaggtggag ggagtccacg acgcaagact
     6961 ccgggtggcg atcgaagcct ctctgcgacg gtcatcggag gagccctcgg gagcttctgg
     7021 tcgcggcacc ccatccggtt gacagaaacc gcctgtttgc ggcgattttg gcgccgccaa
     7081 ggggtcgccc tcaacctcag gttttgatat actcaggcag gcaggtcctg cctgcccccc
     7141 actgccatcg cccagcccct cggccactgt gaggtcgtgt gaagaagtcg aactacgacg
     7201 cccaagacat ccaggtcctc gagggcctcg agccggtccg cgaacggccc ggcatgtaca
     7261 tcggctccac ggggccgaga ggcctccacc acctcgtcta cgaggtgatc gacaactcgg
     7321 ttgacgaggc gctcgcgggg tactgcaccg ccatcgtcgc caccatcaat cccgacaact
     7381 cgatcaccgt cgcggacaac ggccgcggca tccccgtcgg gatcatggag aagtacaacc
     7441 tgcccgccgc caccatcgtg ctcacgatgc tgcacgccgg cggcaagttc ggcggcgcgg
     7501 gctacaaggt ctccggcggg ctgcacggcg tcggcgtatc ggtcgtcaac gcgctcagtg
     7561 agtggctcga gttggacatc tacgtggacg gttaccactg gaaccagcgc ttcgaacgcg
     7621 gcaatccggt caccgagttg gtcaagaggg agaagctcga caaggatgga ccaaccggca
     7681 cgaccgtctc cttcctcgcc gaccccgata tcttcgagga agtcgactac gactaccgcg
     7741 tgctcgccca gcgcctgcgc gagacggcct tcctcaccaa gggtctcaag atctccctga
     7801 acgacgagcg cgccgacggc gagagcgtca ccttccagta cgagggtggc atccgcgact
     7861 tcgtggcgta catcaaccgc gagaaggacg ccatccaccg cagcatcatc tacctcgaga
     7921 acgagacccc cgacggctcg gtagagatcg cgatgcagtg gaactcgtcg tacaacgagt
     7981 ccgtcttcac cttcgccaac aacatcaaca ccaccgaggg cggctcgcac ctcaccggtt
     8041 tccgttccgc gctcacgcgc acgctcaacg actacgcccg gcagaagaac ctcctcaagg
     8101 agaaagagga caacctcacc ggcgaggaca tgcgcgaggg cctcacggcg ctcgtctcgg
     8161 tcaagctcaa gaacccgcag ttcgagggtc agaccaagac caagctgggc aacaccgaga
     8221 tcgccacact ggtggcgacg tcggtcaacg agaagctggc ggagttcctc gaggagaatc
     8281 ctagcgaggg gcgtcagatc gtcgagaagg cgatcaatgc ggcgcgggcg cggagcgccg
     8341 cgcgcaaggc gcgtgacctc acgcgccgca agggcctgct cgaaggcagc acgctgcccg
     8401 gcaagctggc cgactgctcc atcaaggacg cggccctcag cgagatctac cttgtggagg
     8461 gcgactcggc cggtggctcg gccaagcagg cgcgtgaccg ctcgttccag gccatcctgc
     8521 cgctgcgcgg caagatcatc aacgtcgaga aggcgcgcat cgacaagatg ctccagaacc
     8581 aggagatcct gacgatgatc agcgcgatcg gcgccggcat cggcgaggag ttcgacgtcg
     8641 agcaggcgcg ctac
//
LOCUS       DASUMX010000234         3580 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1665129, whole genome shotgun sequence.
ACCESSION   DASUMX010000234 DASUMX010000000
VERSION     DASUMX010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3580
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1665129"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(382..2016)
                     /locus_tag="VFZ86_10920"
     CDS             complement(382..2016)
                     /locus_tag="VFZ86_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP binding domain-containing protein"
                     /protein_id="HEX5642831.1"
                     /translation="MTDYSVSPGGEKVPLPAAEDYEGEYRRLERLASQARADGMEVVV
                     VMGLGFVGAVMAAIVADSVGADGRPTKFVIGCQRPSTRSYWKIGLLNQGISPIKAEDP
                     EVDAIIARCVLEKRTLTATYAGDCLKLADCVVVDVQCDYAKQELGNMRSGQTDMAALE
                     ATLRTIGDRIPERCLVLIETTVAPGTTEFVAWPILKKAFAARGLQRTPLLAHSYERVM
                     PGREYVASIRDFWRVCSGCTEEARARVESFLRAVLNTDEYELTVMDRPIESETAKIVE
                     NSYRATILAFLNEWSIFAERNGVDLLKVVDAIKKRPTHDNMIFPGPGIGGYCLPKDGG
                     LGYWAYKHILGFEDGDQVFRMSTAAIDINDTRSLHVATLTRDALRDMGRYIAGAEVLL
                     CGASYRQDVGDTRYSGSEVVVRKLTEMGAEMRVHDPYVEHWHEFEKQESYPAPGHSLA
                     RFFRNQDHLTSLRVQKDLVEALRGVEALILAVPHAPYLALSPDDVVAWAGKPIAVVDC
                     FGILSDDRIRRYFELGCEVKGLGRGHVQRLKDEVRS"
     gene            2432..>3580
                     /locus_tag="VFZ86_10925"
     CDS             2432..>3580
                     /locus_tag="VFZ86_10925"
                     /inference="COORDINATES: protein motif:HMM:NF012340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin like domain-containing protein"
                     /protein_id="HEX5642832.1"
                     /translation="MDRSSRALLVACVLTLCAALLGGPRAAASGAAPPWESAGPYPAS
                     YDLRDHGKVTGVREQDHKSTCWIIAATGSLESCLLPGALYDFSENNLANHQGSLLDFG
                     GRADSELSTAYFARWDGPVLERQDPYPFPGASPEGLRPALHVQEVLFLPPRVGAGDDA
                     ALKWAITAYGGVDATMCWTVGEDALDRTTYAFSAWQPGLTLNHHVTCVGWDDAYPASS
                     FATRPPGPGAWLVKNTWGTDWGLAGYFWISYHDLYFGGQMAVFTGAEPASNHDAIYQY
                     DALGRSDTYGYGTEAASFASRFSCAGSGTLSAVSFYTPVPDSLYEVRVARSVAELPAA
                     AVAGSGTIPVAGYHTVRFAEPQAVTAGKDFVVAVTLVTPGSTEPVPLEH"
BASE COUNT          488 a         1260 c         1257 g          575 t
ORIGIN      
        1 gggatccgac tatcacggaa ggacgacctt tcgatggagg acaggtgcga ggcgcggatc
       61 gcggccgcgg gatgtgctga tcggtctgcc ttgagcggac ttcaatcgac ggtactcccg
      121 tccttgcgtc ggtctgctcc cggatccgcg gggaaaatgc ctctttctgc agtggaccgc
      181 cgcgtcctcc acgcgctcgt ggactcgcaa aggcccaatg gcgggcagtc tcccagactg
      241 acatcagccg tgcaacccgg cgcgactagc acgaattcca gcggattagt acggcgtggg
      301 ggtgcgccgg cacgggtggc gggacttccc gctgcgggcg gtcgttcctg tcgccgtccg
      361 gcctcgtgcc cgcgccgcgg cctaggagcg cacctcgtcc ttgagccgct gcacgtgccc
      421 gcggccgagc cccttgacct cgcagccgag ctcgaagtac cgcctgatcc tgtcgtcgct
      481 gaggatgccg aagcagtcga ccacggcgat gggcttgccc gcccacgcga cgacatcgtc
      541 cggtgagagc gcgaggtacg gcgcatgcgg caccgccagg atcagcgcct cgactccgcg
      601 cagcgcctcg acgaggtcct tctgcacgcg caggctcgtg aggtggtcct ggttgcggaa
      661 gaaccgcgcc agggagtgtc ccggcgccgg gtaggactcc tgcttctcga actcgtgcca
      721 gtgctcgacg tagggatcgt ggacgcgcat ctcggcgccc atctccgtca gcttgcggac
      781 gacgacctcg gagccgctgt agcgcgtgtc cccgacgtcc tgacggtagc tcgcgccgca
      841 gagcagcacc tcggcgcccg cgatgtagcg ccccatgtcg cgcagtgcgt cgcgcgtcag
      901 cgtcgccacg tggagggagc gggtgtcgtt gatgtcgatc gccgcggtgc tcatcctgaa
      961 cacctggtcg ccgtcctcga agccgaggat gtgcttgtac gcccagtagc ccagaccacc
     1021 gtccttgggc aggcagtacc cgccgatgcc cggcccgggg aagatcatgt tgtcgtgcgt
     1081 cggacgcttc ttgatggcgt cgacgacctt gagcaggtcg acgccgttcc gctccgcgaa
     1141 gatgctccac tcgttgagga aggcgaggat cgtcgcgcgg taggagttct cgacgatctt
     1201 ggcggtctcg gactcgatcg gccggtccat gaccgtgagc tcgtactcgt ccgtgttcag
     1261 caccgccctg aggaagctct cgacgcgggc gcgggcctcc tcggtgcagc cgctgcacac
     1321 gcgccagaag tcgcggatgc tggcgacgta ctcgcggccg ggcatcacgc gctcgtagct
     1381 gtgcgcgagc agcggcgtgc gctgcaggcc gcgggcggcg aacgccttct tgaggatcgg
     1441 ccaggcgacg aactcggtcg ttccgggcgc gacggtcgtc tcgatcagca ccaggcagcg
     1501 ctccgggatc cggtcgccga tcgtcctgag ggtcgcctcg agcgcggcca tatcggtctg
     1561 gccgctgcgc atgttcccga gttcctgctt ggcgtagtcg cactgcacgt ccacgaccac
     1621 gcagtcggca agcttgaggc agtcgccggc gtaggtcgcc gtgagcgtcc tcttctcgag
     1681 cacgcagcgg gcgatgatcg cgtcgacctc ggggtcctca gccttgatcg gggagatgcc
     1741 ctggttgagg aggccgatct tccagtagct gcgcgtgctg gggcgctggc atccgatcac
     1801 gaacttggtc ggccggccgt ccgcaccgac cgagtccgcg acgatggcgg ccatcacggc
     1861 gccgacgaac cccagcccca tgacgacgac gacctccatg ccgtccgcgc gtgcctgcga
     1921 ggccagccgc tcgaggcgcc ggtactcgcc ctcgtagtcc tcggccgcgg ggagcgggac
     1981 cttctcgccg ccgggactca ctgaatagtc ggtcatgacg cgctgccttc ctgccgaggg
     2041 gcgcctgcgc ggcggtgtgc cggccggtcc gccgcgtgtt gcgccattat cggacaggag
     2101 gggccgcctc gaccagcgag gcggcccttc gcgcaccctg tccggccgcg gcggttctcg
     2161 gccgtgttgc cgcgcctgac gacgccaacc gctttctccg gccctccgac gggcctaggc
     2221 tcgttctgcg gccccgcctg ccgattcgcc tggtcttgcc cggcgagacg tctctgccct
     2281 ccaagctggg accatggcgg ggccgcatct tcggtgacgc tccccgcgac ggccgcgggc
     2341 gccgtggtgg ccgtgggcgg tagccttgac gcgagccgcg acgcggggga taagtgtcca
     2401 cgacccacct gagcgcggcc gggaggacgg ggtggaccga tcgtcgagag cactgctggt
     2461 ggcctgcgtc ctgacgctgt gcgccgcgct gctcggcggc ccgagggccg ccgcgtcggg
     2521 cgcggcgccc ccgtgggaat ccgccggacc gtaccccgcc agctacgacc tgcgcgacca
     2581 cggcaaggtc acgggggtcc gggagcagga ccacaagagc acctgctgga tcatcgccgc
     2641 gaccggctcg ctggaatcgt gcctgctccc gggcgcgctc tacgacttct ccgagaacaa
     2701 cctggccaac caccaggggt cgctgctcga cttcgggggc cgcgccgaca gcgagctgtc
     2761 cacagcctac ttcgcgcgct gggacggtcc cgtgctggaa cgccaggacc cgtacccctt
     2821 cccgggggcg tcgcccgagg gcctgcggcc ggcgctgcac gtccaggagg tcctcttcct
     2881 tccgccgcgc gtgggggcgg gggacgacgc ggcgctcaag tgggcgatca ccgcctacgg
     2941 cggcgtcgac gccacgatgt gctggacggt cggcgaggat gcgctggacc ggacgacgta
     3001 cgccttctcc gcgtggcagc cgggactcac gctcaatcac cacgtcacct gcgtgggctg
     3061 ggacgacgcc tatccggcct cgagcttcgc gaccaggccg ccggggccgg gcgcctggct
     3121 ggtcaagaac acctggggca cggactgggg cctcgccggg tacttctgga tctcctacca
     3181 cgacctctac ttcggcgggc agatggccgt gttcacgggc gcggagcctg cgtcgaacca
     3241 cgacgccatc taccagtacg acgccttggg acggagcgac acctacgggt acggcacgga
     3301 ggcggcgtcg ttcgcgagcc gcttctcctg cgccggcagt ggcacactga gtgcggtgag
     3361 cttctacacg ccggtgccgg attcgctgta cgaggtgcgc gtcgcccgct cggtggcaga
     3421 gctcccggcg gcggcggtcg ccggcagcgg gacgatcccg gtggccggct accacaccgt
     3481 ccggttcgcc gaaccgcagg cggtgacggc cggaaaggac ttcgtcgtgg ccgtcaccct
     3541 cgtcaccccg gggtcgacgg agccggtccc gctggagcac
//
LOCUS       DASUMX010000235        28417 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_371989, whole genome shotgun sequence.
ACCESSION   DASUMX010000235 DASUMX010000000
VERSION     DASUMX010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 28417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28417
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371989"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1188)
                     /locus_tag="VFZ86_10930"
     CDS             complement(<1..1188)
                     /locus_tag="VFZ86_10930"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEX5642833.1"
                     /translation="MKAGDLLTISPLIALTLTAVVVMLMAAFWRSHALALGITLGGLA
                     ATFGTLFVAASRTERIVTPLVSMDAYTLFFIGLLTVAAAIVALLSWSYLQQLRVRPEE
                     YYVLLLIATLGGATLVAGAHFASFFLGLEILSVSLYALIVYAVHREDAVEAAVKYLVL
                     AGATSAFLIFGMALVYAESGTMSAGGVGKLIAGLGGGDVVVTIGIVMLLVGVGFKLAV
                     VPFHMWTPDVYEGAPAPVTAYVATVSKGAVFALLLRFMLPVSSDQGSTIFLALTAVAI
                     ASMVAGNLLALRQDNVKRILAYSSIAHLGYLLVAFLASGERAGVAVGFYLVAYFATTL
                     AAFGVVTVLSPEDRDADRIDDYRGLGARRPWLAAIFAVALFSLAGMPLTMGFIGKFVI
                     VAAG"
     gene            complement(1185..2819)
                     /locus_tag="VFZ86_10935"
     CDS             complement(1185..2819)
                     /locus_tag="VFZ86_10935"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEX5642834.1"
                     /translation="MILLWYILIPAVAAPVAWLLGRQNPVLARWTALLGTAAPLALVA
                     GQWIANADALRAPFAPAGPGPAGLVAGSWIADVKAEWIDSLGISFFLAADGLTLIMLL
                     LTFALGLLAVLASWRGITERVGVFHLMVLWTVAGLAGVFLSLDLFLFYFFFEMMLIPM
                     YFLIAVWGHERRVYSAIKFFIFTQVSGLLMLIAIVALVFIHGRATGVYTFDFTQLLGT
                     RMGSTTAMLLMLGFFAAFAVKLPAWPLHTWLPDAHTDAPTAGSVLLAGVLIKIGAYGM
                     IRFMVPLFPDAARDFRVWALILGVVGIIYGAIMAYAQTDMKRLVAYTSVSHMGFVLLG
                     IFAWNTLALQGVILQLVCHALSTGALFILVGGLQDRIHTRDMDKMGGLWQVAPRMGGT
                     AMFFAMASLGLPGLGNFVAEFLILVGVWQVSPWAAVLGAVGLVFATVYALWMMQRAFQ
                     GEETHGLSFVDLTKREIGMFAGMIAALVLLGFWPQPLITTARQTVEGVQVMTSGSTLN
                     RGPDLGGTGDPAEATQAAGAAAAPNGETVAADGGAP"
     gene            complement(2824..4722)
                     /gene="nuoL"
                     /locus_tag="VFZ86_10940"
     CDS             complement(2824..4722)
                     /gene="nuoL"
                     /locus_tag="VFZ86_10940"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEX5642835.1"
                     /translation="MLELLWLIPALPAAGFVLLVLLDRRLGRAGAAWIGAGSVGLSAL
                     VALLVCGSFITSPPAGDAYHQVLWTWMDVAGFAPAIGLYLDAVSVTWVLVITFVGFLI
                     HLYSTEHMEHDEGFTRFFMYLNLFVASMLVLVLADNLLLLYLGWEGVGLCSYLLISFW
                     HTDPQNVYSGRKAFIVTRVGDTALIAGLFLLFTQLHTLEIQPMLAAAEESWASGSAVA
                     VAAALLILGGAVGKSAQVPLQTWLPDAMAGPSPVSALIHAATMVTAGVYLIARTSVIF
                     ELAPDARLVVAIVGTVTLLIAGFSALVQHDLKRALAYSTISQIGYMFLALGVSGWEAG
                     VFHFMTHAFFKALLFLGAGVIIEALADEHDIFKMGGLRRELPAVFWTFTIGAAALAAL
                     PFVTDGFYSKDLIIATSLGTVDGNVWFYVGSLVGAFVTALYAFRLVWLVFFGERRTAV
                     TRRPRWRQMVPLWLLAVLSVVSGFVWMPAWVSGFHPLEDFLDTALPPIEWTGQYAHFA
                     ESAGAALLIIVVVYAGIGISYLVWMPWRRGTHAFAASPAIRPLAAFWLGGWGFDWLYE
                     RVFVLPVKWFARVARNDLVDYVVGGIAWLTSAGWGALSAGQTGRLRAYVAVAGFGIVL
                     VLAVVVLR"
     gene            complement(4725..5033)
                     /gene="nuoK"
                     /locus_tag="VFZ86_10945"
     CDS             complement(4725..5033)
                     /gene="nuoK"
                     /locus_tag="VFZ86_10945"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010960630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEX5642836.1"
                     /translation="MAAVSLDHALVLAAILFAIGFAGLLTRRNLIVVLACIEIMLNAA
                     GLAFVAAGARWGQADGQVMFLFVLAMAAAEVAVGLAIAIRFRQSVKSLDIDDADKMRG
                     "
     gene            complement(5033..5605)
                     /gene="nuoJ"
                     /locus_tag="VFZ86_10950"
     CDS             complement(5033..5605)
                     /gene="nuoJ"
                     /locus_tag="VFZ86_10950"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEX5642837.1"
                     /translation="MTALFYIAAVVAVLSTLMVVTRRHLVHALLYLVISLFAVAVVFY
                     TLGAPFVAALEVIVYAGAIMMLFLFAVMLLNLQADSPLRVPRTAWVGPAILVAILLAE
                     MIYGLTMETQPLAGAEVGPQAVSEALYGPYVLGVELASMLLLAALIGARHVAARTSDE
                     EDAAASMERGRAAAGTPSSAQSPAAEEGDA"
     gene            complement(5602..6138)
                     /gene="nuoI"
                     /locus_tag="VFZ86_10955"
     CDS             complement(5602..6138)
                     /gene="nuoI"
                     /locus_tag="VFZ86_10955"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEX5642838.1"
                     /translation="MPRIGRTVWSTLVGVGVVISHAWRSRDTIQYPEEMVYLPPRYRG
                     RIILSRDPDGEERCVACYLCSAACPVDCIALQATTDENGRRYPEWFRINFSRCIFCGF
                     CEDACPTYAIQLTPDVEMSEYERSSMVYEKEDLLISGPGKYHEHDFYRVAGLAIGGKG
                     KGEAENEAPPSDVRGLLP"
     gene            complement(6257..7213)
                     /gene="nuoH"
                     /locus_tag="VFZ86_10960"
     CDS             complement(6257..7213)
                     /gene="nuoH"
                     /locus_tag="VFZ86_10960"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEX5642839.1"
                     /translation="MAWYWQLLVTLLVLVAVLTIAAGLIWYERRLLSLVQDRYGPNRV
                     GPAGLLQVLADTFKILFKEDWVPPFADKRVFVVAPSLVLITALLSFIVVPVAEGWVVA
                     DLNVGLLFVLAMSSLAVYSVVLAGWSSRSKYSLVGGLRAAAQMVSYEVFMGLSLVGVI
                     TLAGSFNLSDIVEGQQRSVYYVFPQFIGFVVFLVAGLAETRRIPFDLVEAESELVAGY
                     HTEYSGMKFGLFLVGEYVAITLVSALIVTLFFGGWLGPWLPDVVWFLLKTFVFITLFI
                     IIRGTLPRLRYDQLMALGWKAMLPLSLLNLLITGAIVVARAG"
     gene            complement(7213..10146)
                     /locus_tag="VFZ86_10965"
     CDS             complement(7213..10146)
                     /locus_tag="VFZ86_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010960634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX5642840.1"
                     /translation="MSGNGQPPKLVTVIIDGAEYQAEEGRNMLDVALALGFDLPFFCW
                     HPVLGSIGACRQCAVRLYWTDREGREKSEIAMACMTEAQEGTRMDIFDEEAIRFRAEM
                     IELMMLSHPHDCPVCDEGGECHLQDMTVMTGHSYRRYRGRKRTFENQDLGPFVTHELN
                     RCITCYRCTRFYNDYAGGRDFGVFGLRNQVYFGRAESGTLESEFSGNLIEVCPTGVFD
                     DKPFSRRYSRKWDLQTSPSVCPDCGLGCTTTLSARYGELRRVQGRFNRDVNKMFLCDR
                     GRFGFEFVNAPERVRTARIGPGLLPPQTMMGPAIEEPAGAEAAQAEPQPIADAPTEEG
                     ARLQAEAHAAAAPEGQGGQAAGTAALLGELALDAAAGLLRGRRAVGIGSPRASLEANY
                     ALRALVGPSRFFRGMSDTDDRLVGAVLEIAADPRLRLGSAADVHGADAVLVLGEDLTN
                     TAPMLDLTIRTWLHLRPNEVEERNHIRRWNDAGITRIKRREPSALWIATTHATKLDEV
                     AAETQRAAPDDLVRLALALAHEVDAEAPAVPGLGDDEAELVRRWAAALTAGSAPLVVA
                     GCSSGSAELLRASAQLAAALHRAARGESVTEERATLVLTVPEPDSVGLRMLGGGTLVQ
                     ALAAVARGESEVVVVLDDDLDRRAPALLVDDLLRREVPVVALAALEDRLTATAAVVLP
                     AATFAEETGTWVSHEGRAQRYFAALPPADGVRPSWRWLRELLVRLGRREARGWTTPDD
                     VLAALEMELPAFRGAGAAAPAASWRRDGRKVARQPLRWSGRTAVDAGRTVFEPGPAPD
                     ADSALAFSLEGLQPPASPDELRVRTWWPGWNSGNGLHKFGEELTAVGPARPSGVRLLD
                     GAAGHELPPVPAPARFAARDGEAVLVALHHIYGSEQLSMYTPGIRERAPAPFIGLNEE
                     DAERLAVRDGDLLELWLPWMDTRAPFALVPSLVTGTAAVPVGLPGLPYISLPARARLT
                     RAEEQ"
     gene            complement(10143..11495)
                     /locus_tag="VFZ86_10970"
     CDS             complement(10143..11495)
                     /locus_tag="VFZ86_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEX5642841.1"
                     /translation="MTSRPGLPGYPADDRPLTRAMRADGLPATIDDYIAAGGYEGLRR
                     ALEGLRPPEVTTMVTESGLRGRGGAGYSTGRKWSTMPLGADAPHPKYIVCNGDEMEPG
                     SFKDRFLLERNPHLLLEGMLLAGFATESDEGYIFLRQQYDTAVVAIEHAIAEARERGY
                     LGKDVLGSGFPFDIELHVSIGRYICGEASAMLNALESQRANPRARPPHMTGAGLFSRP
                     TVVNNVETYMAVPGIVRHGPDWWKGLSVTDEGGTKIFGVAGRVARPGCVELPMGVPMR
                     ELIEVHAGGMLSGFALRAVSPGGASTSFVPAAQIDVPLGFSTMDEVKSRLGTGTLVLL
                     DDQTCPVGMTLNMMQFFAQESCGWCTPCREGLQWMAALLEDIEYGRGEPGDTGVLAEG
                     VWFMDSDKCFCDLAPGAMQPLESALRLFPDDFARHVAEGGCRYRRHGSDAPALRGTTW
                     "
     gene            complement(11492..11959)
                     /gene="nuoE"
                     /locus_tag="VFZ86_10975"
     CDS             complement(11492..11959)
                     /gene="nuoE"
                     /locus_tag="VFZ86_10975"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019019134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HEX5642842.1"
                     /translation="MSLSDEETRQLDAILGHYPDRRAAAATAMRIVQARRRWLSDEAL
                     ADLGEYLGVTVAELDSIATFGNMVFRKPVGRHIVLVCDSFACWSLGYAELRAAVEAEL
                     GVGMGQTTEDGRFTLLPIVCLGACDRGPAMMVDDELHGPVAPADVAGILGGYA"
     gene            complement(12060..13967)
                     /gene="nuoC"
                     /locus_tag="VFZ86_10980"
     CDS             complement(12060..13967)
                     /gene="nuoC"
                     /locus_tag="VFZ86_10980"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014812487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HEX5642843.1"
                     /translation="MDDAKQLPGDQQPEREDTREEVLDEARAEHAEPVAGAPQPSQWP
                     PRAAGGGEAPPPAVLRELRERFPDVPLTEQPTADGIPTAWVPPGDALAVLRYLGRDAA
                     EPFAMLYDLGGVDERSRLHREGQPDAAFSVFYHLVSYGRNADVRLKVALPEESPHVDS
                     AAGVWPAADWYERELWDMFGVGVAGHPRLKRLLMPPWWDGHPLRKEHPSRGTEYGPFD
                     MPDSLVDAWQEELRFKPEEWGLPGTEDDPTLMYLNIGPQHGATHGPFRVIVGLRDEEI
                     VHLVPDIGYHHRGSEKLAERQSWHTFIPYTDRVDYLGGVTNNLPYVLSVEKLCGIEVP
                     DRAQLIRVLLCEAYRIASHLVFYGTFGQDIGALSPVFYMFEDREILFDDVIEPITGGR
                     MHPSWFRIGGVAEDLPEGWRQRVLDYCDYLPARLDEYDRLLMDNRILKARTVGVAPIS
                     VDDAVAWGLTGPMLRAAGLPWDWRKQRPYSHYDWFDFDVPVGTTGDAYDRAAVHVEEM
                     RQSLRIMRQVVEAMPDGPYKAYHPLATPPMKQPHTMHDIESLITHFLGVTWGPVVPPG
                     EASVYTEGTKGQYSYYTISDGGNSAYRTRIRTASFPHLQVLPLLCTGYEIADLVTVLG
                     SIDFVMADVDR"
     gene            complement(13967..14566)
                     /locus_tag="VFZ86_10985"
     CDS             complement(13967..14566)
                     /locus_tag="VFZ86_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013220840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEX5642844.1"
                     /translation="MLDVEGAPPGDADIPVVTSSLERLVAWGRKNSLWPFGFGLSCCF
                     VEMATSLTARYDIARYGAEVLRISPREADVMIVAGTVFKKMAPVVVQLHRQMMEPRWV
                     ISMGSCANSGGMFDVYSVVQGVDSFLPVDVYIQGCPPPPTSLMEGLLLLQAQVSIEKR
                     PLSWVVGEQGVVKPRGISLRDLRHDDVVATDKLRPPDEM"
     gene            complement(14617..15054)
                     /gene="ndhC"
                     /locus_tag="VFZ86_10990"
     CDS             complement(14617..15054)
                     /gene="ndhC"
                     /locus_tag="VFZ86_10990"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEX5642845.1"
                     /translation="MAVEVWPAALYAALVLVVAALILTLSWLLGGRRYHGPETEPYES
                     GIKPAGPLPRRLSVEFYQVALFFVIFDLEAVFILTWAVAARSLGWLGYLELLVFVLLL
                     FAGLVYLWRVGGLDWGAAGRRRRTAHAAGRKAPAAGDGAGGAR"
     gene            15263..16135
                     /locus_tag="VFZ86_10995"
     CDS             15263..16135
                     /locus_tag="VFZ86_10995"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX5642846.1"
                     /translation="MSRPPVQPPMWGREPGLSWPAPALLVLALVAAVLACVAFAAAGV
                     SVSAAAASAPPPLQSPPAEVLPPQGSGSFTPAATPQPVPSSTYPPGTGGRVYGENCSG
                     CHGARGEGLVGPPLAAAAFASLVGPMVVEGGISMPAFAGVLSDADVDAVSDFVAGELS
                     DPEARTAQVTPGGDLFRVYCSGCHSAIGSGGAMPSLNNAPNIRQYPPAEAIAAMILGR
                     GNMPSFAGNTLDVRQQASVALYVQVIDDPPSPGGFGLGYLGPVPEGAAGAVALLLLIL
                     ISVWLAWRSRKAVP"
     gene            16147..16980
                     /locus_tag="VFZ86_11000"
     CDS             16147..16980
                     /locus_tag="VFZ86_11000"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEX5642847.1"
                     /translation="MTTWPVGLGIVAAIAGAVSFAVCFVIGASNAWLGGSLALALLGL
                     GLALAYWGRDLADDHVIAGRYPVPPDDPEGGAALATQLDEHASVITRRSFLSKALVLG
                     IGVFALSQVVLLGALGPLPGDSLRTTGWRKGRRLVDSEGEPVTRDVLASGGFLVAFPE
                     DGDHLERADSQIVLLHFVNGEFTPQPGRETWSPEDFVAYSRVCTHAGCSVAQYADVDQ
                     LLLCPCHQSTFDVLNGAAPTSGPASRPLPQLPLAIDDEGVLYAQSDFTEPIGPGFWNA
                     V"
     gene            16977..18341
                     /locus_tag="VFZ86_11005"
     CDS             16977..18341
                     /locus_tag="VFZ86_11005"
                     /inference="COORDINATES: protein motif:HMM:NF012263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HEX5642848.1"
                     /translation="MSNHDLKRGLGKAFGVRTSPLRPAGRWLHTNLGGSRLLREELRH
                     IFPDSWAFFLGEIAMYCFVILVATGIFLALFFQASEAPVIYEGSYEPLAGAEMSIAYD
                     SVLQLSLDVPAGLLMRQLHHWAALVFVGALIIHMIRMFVTAAYRRPRRINWLIGLSLV
                     LLVGMNGLMGYSLPDDLLSGTGLRISFAITESIPFVGPALATLLFGGEFPSGGLIARV
                     YPVHIFLLPAAIGGLLGAHLGLLWLQRHTQFPGPGRSDRVIVGTPMVPAYALRTTGYL
                     LLVAGVCTAFAAFVQINPVWLYGPYRAWDATTAAQPDWYMGWLEGGVRMMPGWDIQIG
                     GLLIPALFWPAVVLPGLIFTPLFLVPWIDWVANRDGAFHNVLLLPGQHPARTALAAGF
                     ITFLSLLLVAGGNDVFAFAYGWSQPWLLEVLQWLTIVLPPVVAVVTYALLRGGARRWA
                     STVR"
     gene            complement(18368..19207)
                     /locus_tag="VFZ86_11010"
     CDS             complement(18368..19207)
                     /locus_tag="VFZ86_11010"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HEX5642849.1"
                     /translation="MDYLRAAQPRIVALFLVTVLAAMLLCGSPAAWRALVVLAATALT
                     VGGAALLNNVIERDVDRSMARTRRRPTASGRLAPARGALAGLAAVAAGAAALWAVAGG
                     LAAVFALLGAFYYVVVYTLLLKPRTALSAVPGGVAGVFPALIGWAATGAAWSGAILFL
                     CVLIPVWSPPHSWALTLALGDDYRASGIPTPPARYGEEATRRLIAGFVAVLTVVLAAP
                     LVAGLFGAVYAAGAVAAAVVLWAFTARLLGRPAGGAAWALFKATGPCLALVLAAAVAD
                     QLL"
     gene            19373..20365
                     /gene="coxB"
                     /locus_tag="VFZ86_11015"
     CDS             19373..20365
                     /gene="coxB"
                     /locus_tag="VFZ86_11015"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEX5642850.1"
                     /translation="MSRGPAVAAGAFAVVLAALVLGGCDSPDILRSEGSAGNEIVTLA
                     KWIFGILAFVLITVWGILFFVIVTGKGRREQDASQTKGNLRIEIVWTAIPAVIVTVLF
                     ILTVVITNRIDMPAKGAQFTAIGRQWWWDFDYKDLGFKSPNEIYAPVGRPIYIDVESA
                     DVIHSFWVPQMGGKTDMIPGHVNHVRFVPLKTGEYLGECSEFCGVQHGKMRFLFVVVE
                     PEEYTAWVERQQQPAAKATGPEAVAGEKVIASVGCGSCHAIRGTTLKGTFGPDLTHFG
                     SRRGIGAYTLRNTPENLLRWIQDTQAVKPDSNMPNVPLPLVQQQQLTAYLGELK"
     gene            20362..22833
                     /gene="ctaD"
                     /locus_tag="VFZ86_11020"
     CDS             20362..22833
                     /gene="ctaD"
                     /locus_tag="VFZ86_11020"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HEX5642851.1"
                     /translation="MSLRRAIDRGAAGAPLTGAANSPAAAHAERGGLGAWLTTTDHKK
                     IGLMYIGTALVFFLIAVIFAMLMRTQLIKPEMGLLSPQTYNEIFSMHGTTMVFLFGMP
                     ILIGLANYLVPLQIGARDMAFPRANALSYWLLLFGGLVLYASFLFGGALDTGWFSYAP
                     LTLKAYSPHDGVTFWIVSLVLLGGSSILGSINFIVTCLRFRAKGMGLWQMPIFSVATF
                     INSFLILFAIPSLSAALALLYLDRQFGTSFFDPTGGGDPIIWQHLFWFFGHPEVYILI
                     LPAFGIMSEVVPAFSRKPLFGRSSMVVMLGVIGLLGFLVWAHHMFAAGLPSLFNTIMA
                     GTSMLIAIPTGVKIFNWLATMWGGALRFTTSLLYACGLIALFTVGGITGVTLAVVPWD
                     WQVTDTYYVVAHFHNVLLAGTVFAAFAGIYYWFPKMTGRFLSERIGKAQFWLTAFGFA
                     LTFFPLYALGIMGMPRRVYTYAPDVGWNELNLVSSIGGYILALGFVLLFVNIIRSLRS
                     GEPAGENPWRAWTLEWATTSPPQHGNFARLPEIRSDRPLWDAENGYEDEGGRVEEIAW
                     VDVPAEPEQRTITPFWVAFGILILGIGLLGVPLVFIVGTALILITLAVWMNGRWTEID
                     PTPPPTPDAEHRFSFIGLGTLAFIGSESVFFASLIAAVIHLRIHNSVFGGGSGVEITL
                     PLINTVILFASGVTAHYAQTAYRARRKGGFFFLLVLTIVLGVIFLGGQAWEYWHAGFG
                     LSDSIIASSFYVLTGFHGFHVLCGILALVYLLFRARRELRRRPGERDLPEPTPGTSGL
                     VDGGTYYWHFVDAVWVVVFVVVYLL"
     gene            22850..24556
                     /locus_tag="VFZ86_11025"
     CDS             22850..24556
                     /locus_tag="VFZ86_11025"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HEX5642852.1"
                     /translation="MRRGLGRRLRRRRVPPAGVLAFLLFAAAVGAAALPAAAAAHSAL
                     ESSDPAADASVPVSPRQIVLTFSEAPDAELSLIRVLDAQGAAVPGMSAPQVAPGASTA
                     LQVTPSEPLADGVYTVNWRVVSSVDGHVESGAFAFGVGEQPAPDSAIVVELLHTSPWA
                     TALAGLGRWLLYAGLALLVGAATTSLIVYGGRLPRGGVTVLRAAAAVAVAGLVAMVWS
                     QKVLIGAPSLLPLFVMRQGLLLLGLGVALVLCIGAVVLADLWPARWSLWLLGGAGAAA
                     VLLHVLAGHAASPSSFQWLNLLAQWVHMTAIGVWVGGLFWLLLGFRGRDAAERGAAVG
                     VFTRLATAVLGVVLLTGLARAAAEVGSVSGFYDTRYGITLLVKIALVAGLVGLGALNH
                     FFWAPGVRAASGGAADRRFGLTSRGELGVALAVLAVTAVLSGVVPARLAAADPATSGG
                     STGQISASGSDYATSVRVELTLTPGVAGRNDYVLWADDYDTGDPLETVTAVSLKCALP
                     ARPSVAPVTVELTKAPDGSWTGGGLDFSVAGRWQVDVAIQEEAKGSVVPLELTVPAAS
                     SP"
     gene            24676..25332
                     /locus_tag="VFZ86_11030"
     CDS             24676..25332
                     /locus_tag="VFZ86_11030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642853.1"
                     /translation="MRRSATAVLTTALMLVLLGVLAAPVLAHETASAGGVNLELGWGT
                     EPAYQGQLNTIQLIVTHKADGDPVNDPGARLTTTVTYGDQEQEFALTPTYDPDYGTGT
                     PGEYAALVIPTAPGDYTFHVTGTVEGAKVDLKVTSSPKTFSPVEDASAVQFPVKVPAT
                     DQVAERLDKELPRMAAAAEVADMESQVSSATTLGYVGIAVGAVGIVLAAVALLMRRRA
                     "
     gene            complement(25397..26068)
                     /locus_tag="VFZ86_11035"
     CDS             complement(25397..26068)
                     /locus_tag="VFZ86_11035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642854.1"
                     /translation="MLVATFGPTTAWQGREIIWDVDRFILVGHGAIPAAGVLDYDRLG
                     QLVWAQPELRAWVAEVHRWETGGQPATAGAAPAGGAGASGRRGLPAWAIVLIVVAVAF
                     VVLSAILGAVLPAFIVRTTESFGNDAVVRSGVQTIHAGIESYAADHDGQFPPAGEVNA
                     VGMRAYIPAWPVNPYNNLPMADGGGAGNYRYDVSADGGVYKLTGYGSDGQVVIDLSGG
                     SGQSV"
     gene            complement(26109..26600)
                     /locus_tag="VFZ86_11040"
     CDS             complement(26109..26600)
                     /locus_tag="VFZ86_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642855.1"
                     /translation="MDVGATGRLQRDPGQSRPQTHASATYPPDAERCAAELLSIVAYL
                     TAHDGPLQASPDADTAISPWHERLLRKAAERLDVIDAFMRARVAFLAAGKDRGYYAPE
                     AELALAGATLDATRAATAVVAALLESRLPSPHAFDAMSAAALGIIPALDALGEATPSP
                     HAR"
     gene            complement(26667..27002)
                     /locus_tag="VFZ86_11045"
     CDS             complement(26667..27002)
                     /locus_tag="VFZ86_11045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642856.1"
                     /translation="MELRETIDPGICGYPVVVTARTEDARHVEFSFESECELLAEFAR
                     RIAEISPVDAIQSLSREENPVLAQARELLRTAGCCEACVVPVGAVKAMYVITDLALPR
                     DVTLTLTSE"
     gene            complement(27037..27852)
                     /gene="ric"
                     /locus_tag="VFZ86_11050"
     CDS             complement(27037..27852)
                     /gene="ric"
                     /locus_tag="VFZ86_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein"
                     /protein_id="HEX5642857.1"
                     /translation="MTKVKIAAPLPPGMVRGVLHVTIEETSVRDEDPTPETSPVGDIV
                     AGDYRTAAVFERHGIDFCCGGAVTLAAACAAHRVDPSVLLRELEAATRTPARPDADYG
                     NWTLTRLIDHIKAIHHAYVRANVAQTAAYTRKIADVHGAQHPELAEIVAAFDQMATEL
                     TAHLEQEEAVVFPAIKRAEAAGRTGAAPDAADAETIARGIEALVREHEEVGTALHHIR
                     DLAMGYGLPPDACATWSLTYERLQAFEADLHKHVHLENNLLFPRAGDRFVPVA"
     gene            complement(27943..28140)
                     /locus_tag="VFZ86_11055"
     CDS             complement(27943..28140)
                     /locus_tag="VFZ86_11055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642858.1"
                     /translation="MKKFAVTILAAGALLVVPSSALAANASGDPGEAFGQHHAAMAQD
                     GHLGADMTPGMHKGFSGWTPM"
BASE COUNT         4121 a        10042 c        10281 g         3973 t
ORIGIN      
        1 gccggcggcg acgatcacga acttgccgat gaaccccatc gtcagcggca tgccggccag
       61 cgagaagagc gcgacggcga agatggcggc cagccacggc cgtcgcgcgc ccagcccgcg
      121 atagtcgtcg atcctgtcgg cgtcgcggtc ctccggggag agcacggtga ccacgccgaa
      181 ggcggcgagc gtggtggcga agtaggcgac gaggtagaag ccgacggcga cgccggcgcg
      241 ctcgccgctg gcgaggaacg ccaccagcag gtagccgagg tgggcgatcg aggagtacgc
      301 caggatccgc ttgacgttgt cctggcgcag cgccagcagg ttgccggcga ccatggaggc
      361 gatggcgacc gcggtgaggg cgaggaagat ggtcgagccc tggtcggagc tgacggggag
      421 catgaagcgc agcaggaggg cgaacacggc ccccttggag acggtcgcca cgtaggcggt
      481 gaccggcgcg ggcgcgccct cgtacacgtc cggcgtccac atgtggaagg gcacgacggc
      541 cagcttgaag ccgacgccga cgagcagcat cacgatgccg atggtgacga ccacgtcgcc
      601 cccgccgagg ccggcgatca gcttgccgac gccgccggcc gacatcgtcc cggactccgc
      661 gtacaccagc gccatgccga agatgaggaa tgccgaggtg gctccggcga gcaccaggta
      721 cttcaccgcc gcctcgaccg cgtcctcgcg gtgcaccgcg tagacgatca gcgcgtagag
      781 cgagacgctc aggatctcga gcccgaggaa gaacgaggcg aagtgcgcgc cggccaccag
      841 cgtcgccccg ccgagtgtgg cgatgagcag cagcacgtaa tactcctcgg gccgcacgcg
      901 caactgctgc aggtagctcc acgagagcag cgccacgatc gccgcggcga ccgtgagcaa
      961 gccgatgaag aacagcgtgt aggcgtccat cgagacgagc ggcgtgacga tgcgctcggt
     1021 gcgggaggcc gcgacgaaga gcgtgccgaa cgtcgcggcc agcccaccga gcgtgatccc
     1081 cagcgccagg gcgtgagagc gccagaacgc cgccatcagc atcaccacga ccgccgtcag
     1141 cgtcagggcg atgagcggcg agatggtgag gaggtcgccc gccttcacgg cgcgcctccg
     1201 tcagcggcga cggtctctcc gttcggggcg gcagccgcgc ctgcggcctg cgtggcctcg
     1261 gccgggtcgc cggtcccacc caggtcgggc ccccgattca gcgtgctgcc gctcgtcatc
     1321 acctggacgc cctccaccgt ctgtctggcc gtggtgatca gcggttgcgg ccagaagccg
     1381 agcagcacga gcgcggcgat catgccggcg aacatgccga tctcccgctt ggtcaggtcg
     1441 acgaacgaca agccgtgtgt ctcctccccc tggaaggcgc gctgcatcat ccacagcgcg
     1501 tagacggtcg cgaacaccag tccgacggcg ccgaggacgg ccgcccacgg gctcacctgc
     1561 cagacgccga ccaggatgag gaactcggcg acgaagttgc cgagccccgg caggccgagc
     1621 gaggccatgg cgaagaacat ggccgtgccg cccatccggg gcgccacctg ccagaggccg
     1681 cccatcttgt ccatgtcgcg cgtgtggatg cggtcctgca ggcctcctac caggatgaag
     1741 agggcgccgg tggagagggc gtggcacacg agttgcagga tcacgccctg caacgcgagc
     1801 gtgttccagg cgaagatgcc cagcagcacg aaacccatgt ggctgacgct ggtgtaggcg
     1861 accagccgct tcatgtcggt ctgggcgtac gccatgatcg cgccgtagat gatcccgacc
     1921 acgccgagga tcagcgccca cacgcggaag tcgcgggcgg cgtccgggaa cagcggcacc
     1981 atgaagcgga tcatcccgta ggcgccgatc ttgatgagca cgccggccag cagcacggag
     2041 ccggcggtcg gcgcgtcggt gtgcgcgtcc ggcagccagg tgtgcagcgg ccacgccggg
     2101 agcttgacgg cgaaggcggc gaagaacccg agcatgagca gcatggccgt ggtcgagccc
     2161 atccgggtgc ccagcaactg cgtgaagtcg aaggtgtaga cgccggtcgc gcgcccgtgg
     2221 atgaacacca gcgcgacgat ggcgatcagc atgagcagcc cgctgacctg cgtgaagatg
     2281 aagaacttga tcgccgagta cacacgccgc tcgtggcccc agacggcgat caggaagtac
     2341 atcgggatga gcatcatctc gaagaagaag tagaagagga acaggtcgag cgagaggaag
     2401 acgccggcca gcccggccac cgtccagagg accatcaggt ggaagacgcc gacgcgctcg
     2461 gtgatgccgc gccacgaggc gagcacggcg agcaggccga gcgcgaaggt gagcagcagc
     2521 atgatgagcg tgaggccgtc ggcggcgagg aagaagctga tgcccagcga gtcgatccac
     2581 tccgccttga cgtcggcgat ccaggagccg gccaccaggc cggccgggcc gggacctgcc
     2641 ggcgcgaagg gggcccgcag ggcgtccgcg ttcgcgatcc actgcccggc caccagcgcc
     2701 agcggggcgg ccgttcccag cagcgccgtc cagcgcgcga gcacggggtt ctggcgcccg
     2761 agcagccagg cgacgggtgc tgcgaccgcc gggatcagga tgtaccagag gaggatcacc
     2821 gcgtcacctc agcaccacga cggcgaggac gaggacgatg ccgaacccgg cgacggcgac
     2881 gtaggcgcgc aggcgccccg tctggccggc gctcagcgcg ccccagccgg cgctggtgag
     2941 ccaggcgatg ccgccgacca cgtagtcgac gaggtcgttg cgggcgacgc gcgcgaacca
     3001 cttgaccggg agcacgaaga cgcgctcgta gagccagtcg aagccccagc cgccgagcca
     3061 gaaggcggcc agcggccgga tggcgggtga ggcggcgaag gcgtgcgtcc ctcggcgcca
     3121 cggcatccag acgaggtagg agatgccgat gccggcgtag acgacgacga tgatcaggag
     3181 ggccgcgccg gcgctctcgg cgaagtgcgc gtactgcccg gtccactcga tggggggcag
     3241 cgccgtgtcg aggaagtcct cgagcgggtg gaacccgctg acccacgccg gcatccagac
     3301 gaagccgctg acgacggaga gcacggcgag cagccagagc gggaccatct gccgccagcg
     3361 cggccgtcgc gtgaccgcgg tgcgccgctc gccgaagaac acgagccaca cgaggcggaa
     3421 cgcgtagagc gcggtgacga acgcgccgac cagcgagccg acgtagaacc agacgttgcc
     3481 gtccaccgtg ccgaggctcg tggcgatgat gaggtccttg ctgtagaagc cgtcggtgac
     3541 gaacggcagg gcggcgaggg cggcggcgcc gatggtgaag gtccagaaca cggcgggcag
     3601 ctcgcgacgg agcccgccca tcttgaagat gtcgtgctcg tcggcgagcg cctcgatgat
     3661 cacgccggcg ccgaggaaca gcagagcctt gaagaaggcg tgcgtcatga agtggaacac
     3721 gcccgcctcc cagcccgaga cgccgagcgc caggaacatg tagccgatct ggctgatggt
     3781 cgagtaggcg agcgcgcgct tgaggtcgtg ctgcaccagc gcgctgaagc cggcgatgag
     3841 cagcgtcacc gtgccgacga tcgcgaccac cagcctggcg tccggcgcga gctcgaagat
     3901 gacgctggtg cgcgcgatga ggtagacgcc ggcggtgacc atcgtcgccg cgtggatgag
     3961 cgcgctgacc ggcgacgggc cggccatcgc gtccgggagc caggtctgca gcggcacctg
     4021 cgccgacttg ccgacggcgc cgccgaggat gagcagcgcg gcggcgaccg cgacggccga
     4081 gccggaggcc cagctctcct cggccgccgc cagcatgggc tggatctcaa gcgtgtgcag
     4141 ctgcgtgaac agcaggaaga ggccggcgat cagcgccgtg tcgccgacgc gcgtgacgat
     4201 gaacgccttg cggccgctgt acacgttctg tgggtccgtg tgccagaagg agatgagcag
     4261 gtagctgcag aggcccacgc cctcccatcc caggtagagg agcagcaggt tgtcggccag
     4321 caccaggacg agcatcgagg cgacgaacag gttgaggtac atgaagaacc gggtgaagcc
     4381 ctcgtcgtgc tccatgtgct cggtggagta gaggtggatg aggaagccga cgaaggtgat
     4441 gaccagcacc caggtgacgc tgacggcgtc gaggtagagg ccgatcgcgg gggcgaagcc
     4501 ggcgacgtcc atccaggtcc agaggacctg gtggtacgcg tcgccggccg gcggcgaggt
     4561 gatgaagctg ccgcagacga gcagcgccac cagcgccgag aggccgacgg agccggcgcc
     4621 gatccaggcg gcgccggcgc ggccgagccg ccggtccagc aggacgagca gcacgaagcc
     4681 ggcggcgggg agtgcgggga tgagccagag gagctcgagc atcgtcagcc cctcatcttg
     4741 tcggcgtcgt cgatgtcgag gctcttcacc gactggcgga agcggatggc gatggcgagg
     4801 cccaccgcca cctcggcggc cgccatcgcc agcacgaaga ggaacatgac ctggccgtcg
     4861 gcctgacccc agcgcgcgcc ggccgccacg aaggcgaggc ctgcggcgtt cagcatgatc
     4921 tcgatgcacg ccagcaccac gatgaggttg cggcgcgtga gcagcccggc gaacccgatg
     4981 gcgaagagga tcgccgcgag cacgagggcg tggtcgaggg agacggcggc catcaggcgt
     5041 cgccctcctc ggccgccggg ctctgcgcgg aggacggcgt gccggcggcg gcgcggccgc
     5101 gctccatgga cgccgccgcg tcctcctcgt cgctcgtgcg cgccgccacg tggcgcgcgc
     5161 cgatgagcgc ggcgaggagc agcatggaag cgagctccac gccgaggacg taaggcccgt
     5221 agagcgcctc gctgaccgcc tgcggtccca cctcggcgcc ggccagcggc tgcgtctcca
     5281 tggtgaggcc gtagatcatc tcggccagca ggatggccac gaggatcgcc ggccccaccc
     5341 acgccgtgcg cggtacccgc agcggcgagt cggcctgcag gttgagcagc atcacggcga
     5401 agaggaacag catcatgatg gcgccggcgt acacgatcac ctcgagcgcc gcgacgaacg
     5461 gcgcgcccag tgtgtagaag accacggcca cggcgaacag cgagatcacg aggtacagga
     5521 gcgcgtgcac gaggtggcgg cgcgtcacca ccatcagcgt gctgagcacg gccaccaccg
     5581 cggcgatgta gaagagcgcg gtcacggcag gagacccctc acatccgagg gtggcgcctc
     5641 gttctcggcc tcgcccttgc ccttgccgcc gatggcgagg ccggccaccc ggtagaagtc
     5701 gtgctcgtgg tacttgccgg ggcccgagat gagcaggtcc tccttctcgt acaccatgct
     5761 ggaacgctcg tactcgctca tctccacgtc gggggtgagc tggatggcgt aggtcgggca
     5821 cgcgtcctcg cagaaaccgc agaagatgca gcgcgagaag ttgatgcgga accactcggg
     5881 gtagcggcgg ccgttctcgt cggtggtcgc ctgcagggcg atgcagtcca ccgggcaggc
     5941 cgcggagcac aggtaacagg cgacgcagcg ctcctcgccg tccgggtcgc gcgagaggat
     6001 gatgcgcccg cggtagcgcg gcggcaggta gaccatctcc tccgggtact ggatggtgtc
     6061 gcggctgcgc caggcgtggc tgatcacgac gccgacgccg acgagcgtgg accacacggt
     6121 gcgcccgatc cgcggcagca gcggctggcc gcggcgcgcc agcccgccgg tgacgaccac
     6181 gtccgtggtc agcggcgggt gggtctccac cggcgccggc gggcgcgact gccgcgtgcc
     6241 gtcgctcgtc atctcatcac ccggccctcg ccacgacgat cgctccggtg atcaggaggt
     6301 tgaggaggct gagcggcagc atcgccttcc agccgagcgc catcagctgg tcgtagcgca
     6361 gccgcgggag cgtgccgcgg atgatgatga acagcgtgat gaagacgaac gtcttgagca
     6421 ggaaccagac gacgtcgggc agccacgggc cgagccagcc gccgaagaac agggtgacga
     6481 tgagcgcgga gaccagcgtg atcgccacgt actcgccgac gaggaacagg ccgaacttca
     6541 tgcccgagta ctcggtgtgg tagccggcga ccagctcgct ctcggcctcg acgaggtcga
     6601 aggggatgcg gcgcgtctcg gcgaggccgg cgacgaggaa gacgacgaag ccgatgaact
     6661 gggggaagac gtagtagacg ctgcgctgct gtccctcgac gatgtccgag aggttgaagg
     6721 agcccgccag cgtgatcacc ccgacgagcg agaggcccat gaagacctcg tagctcacca
     6781 tctgggcggc ggcgcgcagg ccaccgacca gggagtactt gctgcgcgac gaccagccgg
     6841 cgagcacgac gctgtacacg gccagcgacg acatcgcgag cacgaagagc agcccgacgt
     6901 tgaggtcggc gacgacccag ccctcggcga cgggcacgac gatgaacgag agcagcgccg
     6961 tgatcagcac cagggacggc gcgaccacga acacgcgctt gtcggcgaac ggcgggaccc
     7021 agtcctcctt gaagaggatc ttgaaggtgt ccgccagcac ctggagcagg cccgccgggc
     7081 cgacgcggtt ggggccgtag cggtcctgca cgaggctgag cagccggcgc tcgtaccaga
     7141 tgagcccggc ggcgatggtc agcacggcga ccagcacgag cagggtgacg agcagctgcc
     7201 agtaccaggc catcactgct cctccgcgcg cgtgagccgc gcgcgcgccg gcaacgagat
     7261 gtacgggagg ccggggagtc cgacgggcac cgcggccgtg cccgtcacca gcgacggcac
     7321 cagcgcgaag ggcgcgcgcg tgtccatcca gggcagccag agctcgagca ggtcgccgtc
     7381 gcgcaccgcg agccgctcgg cgtcctcctc gttgaggccg atgaagggcg ccggcgcccg
     7441 ctcgcggatg cccggtgtgt acatgctgag ctgctcggag ccgtagatgt ggtgcagcgc
     7501 gacgagcacc gcctcgccgt cgcgcgccgc gaaccgcgcc ggcgccggga ccggcggcag
     7561 ctcgtggccg gcggcgccgt cgagcaggcg gacgccggac ggcctggccg ggccgacggc
     7621 cgtgagctcc tcgccgaact tgtgcaggcc gttgcccgag ttccagcccg gccaccaggt
     7681 gcggacgcgg agctcgtccg gtgatgccgg cggctgcagg ccctcgagcg agaacgcgag
     7741 cgccgagtcc gcgtcgggcg ccggcccggg ctcgaacacg gtgcggccgg cgtcgacggc
     7801 ggtgcgcccg ctccagcgca gcggctggcg ggcgaccttg cggccgtcac gccgccacga
     7861 ggcggccggc gcggcggcgc ctgcgccgcg gaacgccggc agctccatct cgagcgcggc
     7921 cagcacgtcg tccggcgtcg tccagccgcg cgcctcgcgg cgcccgagcc gcacgagcag
     7981 ctcacgcagc cagcgccagg acgggcgcac gccgtccgcc ggcggcaggg cggcgaagta
     8041 gcgctgcgcc ctgccctcgt ggctcaccca ggtcccggtc tcctccgcga acgtcgccgc
     8101 cggcagcacc acggcggccg tggcggtcag gcggtcctcc agcgccgcca gcgcgaccac
     8161 gggcacctcg cggcgcagca ggtcgtcgac gagcagtgcg ggggcacgcc ggtcgaggtc
     8221 gtcgtcgagc accacgacga cctcgctctc tccgcgcgcg acggcggcca gcgcctgcac
     8281 gagcgtgcct cctccgagca tgcgcaggcc cacgctgtcg ggctccggga cggtgaggac
     8341 gagcgtcgcg cgctcctcgg tgacgctctc tccgcgcgcg gcacggtgga gtgccgcggc
     8401 gagctgcgcc gaggcgcgga gcagctcggc cgacccgctc gagcagccgg cgacgaccag
     8461 gggcgccgag ccggcggtga gcgccgccgc ccagcggcgc accagctcgg cctcgtcgtc
     8521 gccgaggccg gggacggccg gcgcctcggc gtcgacctcg tgcgcgagcg cgagcgccag
     8581 gcgcaccagg tcgtcgggcg ccgcccgctg cgtctcggcg gcgacctcgt ccagcttggt
     8641 cgcgtgcgtg gtcgcgatcc agagcgcgct cggctcgcgg cgcttgatgc gcgtgatgcc
     8701 ggcgtcgttc cagcgacgga tgtggttgcg ctcctccacc tcgttggggc gcaggtgcag
     8761 ccacgtgcgg atggtgaggt cgagcatcgg cgccgtgttg gtgaggtcct cgccgaggac
     8821 gagcacggcg tcggcgccgt ggacgtcggc cgccgacccc agccgcaggc gcgggtcggc
     8881 ggcgatctcg agcacggcgc cgaccaggcg gtcgtcggtg tcggacatgc cccggaagaa
     8941 cctgctgggg ccgacgagcg cgcgcagcgc gtagttggcc tccagcgagg cgcgcggcga
     9001 gccgatgccc acggcgcggc ggccgcgcag caggcccgcc gcggcgtcga gcgccagctc
     9061 tccgaggagg gcggccgtgc ctgcggcctg gccgccctgc ccttccggcg ccgccgcggc
     9121 gtgcgcctca gcctgcaggc gcgcgccctc ttccgtcggc gcatcggcga tcggctgcgg
     9181 ctccgcctgc gctgcctccg cgccggccgg ctcctcgatg gccggcccca tcatcgtctg
     9241 cggcgggagc agcccgggcc cgatccgggc cgtgcgcacc cgctccggcg cgttgacgaa
     9301 ctcgaagccg aagcggccgc ggtcgcagag gaacatcttg ttgacgtcgc ggttgaagcg
     9361 cccctggacc cggcgcagct cgccgtagcg cgccgagagc gtcgtggtgc agcccagccc
     9421 gcagtccggg cacaccgacg gcgacgtctg caggtcccat ttgcgcgagt agcggcgcga
     9481 gaacggcttg tcgtcgaaca cgccggtggg gcagacctcg atgaggttgc cgctgaactc
     9541 gctctccagg gtgccgctct cggcgcgccc gaagtacacc tggttgcgga ggccgaacac
     9601 gccgaagtcg cggccgccgg cgtagtcgtt gtagaagcgc gtgcagcggt agcaggtgat
     9661 gcagcggttg agctcgtggg tcacgaacgg gccgagatcc tggttctcga aggtccgctt
     9721 gcggccgcgg tagcggcggt agctgtgccc cgtcatgacg gtcatgtcct gcaggtggca
     9781 ctcgccgccc tcgtcgcaga ccgggcagtc gtgcgggtgg ctgagcatca tgagctcgat
     9841 catctcggcg cggaagcgga tggcctcctc gtcgaagatg tccatcctcg tgccctcttg
     9901 cgcctcggtc atgcaggcca tggcgatctc gctcttctcc ctgccctcgc ggtcggtcca
     9961 gtagaggcgc acggcgcact ggcggcaggc gccgatggac cccagcacgg ggtgccagca
    10021 gaagaagggc aggtcgaagc ccagggcgag agcgacgtcg agcatgttgc ggccctcttc
    10081 ggcctggtac tcggcgccgt cgatgatgac ggtgacgagc ttcggcggct gtccgttgcc
    10141 gctcaccacg tcgtcccccg cagcgccggc gcatcggagc cgtggcgccg gtagcggcag
    10201 ccgccctcgg cgacgtggcg cgcgaagtcg tccgggaaca ggcgcagcgc gctctcgagc
    10261 ggctgcatgg cgcccggcgc caggtcgcag aagcacttgt cggaatccat gaaccagacg
    10321 ccctcggcga gcacgccggt gtcgcccggc tcgcctcggc cgtactcgat gtcctcaagc
    10381 agcgcggcca tccactgcag gccctcgcgg cagggcgtgc accacccgca cgactcctgg
    10441 gcgaagaact gcatcatgtt gagcgtcatg ccgacggggc aggtctggtc gtcgaggagg
    10501 acgagggtcc cggtgccgag gcggctcttc acctcgtcca tggtggagaa ccccagggga
    10561 acgtcgatct gggcggccgg gacgaacgag gtggaggcgc cgcccgggct gacggcgcgc
    10621 agcgcgaacc cggacagcat gccgccggcg tgcacctcga tgagctcccg catcggcacg
    10681 cccatcggga gctcgacgca gccgggcctg gccacgcggc cggcgacgcc gaagatcttg
    10741 gtgccgccct cgtccgtgac ggagaggccc ttccaccagt ccgggccgtg ccgcacgatg
    10801 ccgggcacgg ccatgtaggt ctcgacgttg ttgacgaccg tcggccgcga gaagaggccc
    10861 gcgccggtca tgtgcggcgg ccgggcgcgc gggttggcgc gctggctctc cagcgcgttc
    10921 agcatcgcgg aggcctcgcc gcagatgtag cggccgatgc tcacgtgcag ctcgatgtcg
    10981 aaggggaagc cggaaccgag cacgtccttg ccgaggtagc cgcgctcgcg ggcctcggcg
    11041 atcgcgtgct cgatcgcgac caccgccgtg tcgtactgct ggcgcaggaa gatgtagccc
    11101 tcgtccgact cggtggcgaa gcccgcgagc agcatgccct ccagcagcag gtgggggttg
    11161 cgctccagga ggaaccggtc cttgaagctg cccggctcca tctcgtcccc gttgcagacg
    11221 atgtacttgg ggtgcggcgc gtccgcgccg agcggcatcg tgctccactt gcggccggtg
    11281 gagtagccgg cgccgccgcg gccgcgcagg cccgactcgg tcaccatggt ggtgacctcg
    11341 ggcggccgca ggccctcgag cgcccgccgg aggccctcgt aaccgccggc ggcgatgtag
    11401 tcgtcgatcg tcgcgggcag gccgtcggcg cgcatggcgc gcgtcagcgg cctgtcgtcg
    11461 gccgggtagc ccgggagtcc cggccggctg gtcacgcgta ccccccgagg atgccggcca
    11521 cgtcggccgg cgccaccggg ccgtgcagct catcgtccac catcatggcc gggccgcggt
    11581 cgcaggcgcc gaggcagacg atcggcagca gcgtgaagcg gccgtcctcc gtcgtctgcc
    11641 ccatgccgac gccgagctcc gcctccacgg cggcgcgcag ctcggcgtag cccaggctcc
    11701 agcaggcgaa gctgtcgcag acgagcacga tgtggcggcc caccggcttg cggaacacca
    11761 tgttgccgaa ggtcgcgatg gagtcgagct ccgcgaccgt caccccgagg tactcgccga
    11821 ggtccgccag cgcttcgtcc gagagccagc gccgccgcgc ctgcacgatg cgcatcgccg
    11881 tcgcggcggc cgcccgccgg tccgggtagt gcccgaggat ggcgtccagc tggcgggtct
    11941 cttcgtcgga gaggctcatg acgcccactc ttccggcgta cacgagcggg acgccgtagg
    12001 cattgacccg cgtagccgag tcaactggcg gtgcgccgga ggtacaccgg atataccgct
    12061 catcggtcca cgtccgccat gacgaagtcg atgctgccga gcacggtcac gaggtcggcg
    12121 atctcgtagc ccgtgcagag cagcggcagg acctgcaggt gcgggaacga cgcggtgcgg
    12181 atcctggtcc ggtacgccga gttgccgccg tcgctgatgg tgtagtagct gtactggccc
    12241 ttggtgccct ccgtatagac gctcgcctct ccgggcggca ccaccggccc ccaggtgacg
    12301 cccaggaagt gggtgatgag cgactcgatg tcgtgcatcg tgtgcggctg cttcatcggc
    12361 ggcgtcgcca gcgggtggta cgccttgtac ggcccgtcgg gcatggcctc cacgacctgg
    12421 cgcatgatgc gcaggctctg gcgcatctcc tcgacgtgca cggcggcgcg gtcgtaggcg
    12481 tcgccggtgg tcccgacggg cacgtcgaag tcgaaccagt cgtagtggct gtacgggcgc
    12541 tgcttgcgcc agtcccaggg cagaccggcc gcccggagca tcgggccggt cagcccccag
    12601 gcgacggcgt cgtccacgga gatcggcgcg acccccaccg tccgcgcctt caggatccgg
    12661 ttgtccatca gcagccggtc gtactcgtcg aggcgcgccg gcaggtagtc gcagtagtcg
    12721 agcacgcgct gccgccaccc ctcgggaagg tcctccgcga cgccgccgat gcggaaccag
    12781 ctgggatgca tgcgcccgcc ggtgatgggc tcgatgacgt cgtcgaagag gatctccctg
    12841 tcctcgaaca tgtagaagac cggcgagagc gcgccgatgt cctggccgaa cgtgccgtag
    12901 aagacgaggt ggctggcgat gcggtaggcc tcgcagagca gcacgcggat gagctgcgcc
    12961 ctgtccggca cctcgatgcc gcagagcttc tcgaccgaga gcacgtaggg caggttgttg
    13021 gtcacgccgc ccaggtagtc cacgcgatcg gtgtagggga tgaaggtgtg ccagctctgg
    13081 cgctcggcca gcttctccga gccgcggtgg tggtagccga tgtcggggac gaggtggacg
    13141 atctcctcgt cgcgcaggcc gacgatcacg cggaacggcc cgtgcgtggc gccgtgctgc
    13201 gggccgatgt tgaggtacat gagcgtgggg tcgtcctcgg tgcccggcag gccccactcc
    13261 tccggcttga agcggagctc ctcctgccag gcgtcgacca gggagtccgg catgtcgaag
    13321 gggccgtact cggtgccgcg ggacgggtgc tccttgcgca gcgggtgccc gtcccaccac
    13381 ggcggcatga ggagacgctt gagacgcggg tggccggcga cgccgacgcc gaacatgtcc
    13441 cagagctcgc gctcgtacca gtcggcggcg ggccacacgc cggccgccga gtcgacgtgc
    13501 ggcgactcct ccggcagcgc caccttgagc cgcacgtcgg cgttccgccc gtaggagacg
    13561 aggtggtaga agacgctgaa ggcggcgtcg ggctggccct cgcggtggag ccgcgaacgc
    13621 tcgtcgacgc cgccgaggtc gtagagcatc gcgaacggct cggcggcgtc gcgcccgagg
    13681 tagcgcagca ccgcgagggc gtccccgggc ggcacccacg cggtcgggat accgtcggcg
    13741 gtcggctgct ccgtcagcgg cacgtccggg aaccgctcgc gcagctcgcg cagcacggcc
    13801 ggcggcgggg cttcgcctcc gccggccgcg cgcggcggcc actggctcgg ctggggggcg
    13861 ccggcgacgg gctcggcgtg ttccgcacgc gcctcgtcga gcacctcttc gcgcgtatcc
    13921 tcgcgctccg gttgctggtc gcccggcagt tgcttcgcgt cgtccatcac atctcgtccg
    13981 ggggccggag cttgtcggtc gccaccacgt cgtcgtggcg caggtcgcgc agcgagatcc
    14041 cgcgcggctt caccacgccc tgctcgccga ccacccagct gagcgggcgc ttctcgatcg
    14101 agacctgggc ctgcaggagc agcaggccct ccatgagcga ggtgggcggc ggcgggcagc
    14161 cctggatgta cacgtcgacg ggcaggaacg agtccacgcc ctgcacgacg ctgtacacgt
    14221 cgaacatgcc gccggagttg gcgcaggagc ccatggagat gacccagcga ggctccatca
    14281 tctgccggtg cagctgcacg acgaccggcg ccatcttctt gaacaccgtg cccgcgacga
    14341 tcatgacgtc cgcctcccgc ggcgagatgc gcagcacctc ggcgccgtag cgggcgatgt
    14401 cgtagcgggc cgtgaggctg gtcgccatct cgacgaagca gcagctgagg ccgaacccga
    14461 acggccacag ggagttcttg cgtccccagg cgaccaggcg ctcgagactg gaagtgacga
    14521 ccgggatgtc ggcgtcgccc ggcggcgcgc cctcgacgtc gagcagccgc ccgagcgccc
    14581 gggcggtccg cgacgggtcg ggtccccggc gaggcttcac cgggcgcccc ccgcgccgtc
    14641 acccgcggcg ggcgccttgc ggccggcggc gtgcgccgtc ctgcgacgcc ggcccgcggc
    14701 cccccagtcg agcccgccga cccgccacag gtacacgagg ccggcgaaga gcagcaggac
    14761 gaagacgagc aactccaggt agccgagcca gccgaggctg cgcgccgcga ccgcccaggt
    14821 gaggatgaac acggcctcca ggtcgaagat cacgaagaag agggcgacct ggtagaactc
    14881 gaccgagagc cggcgcggca gcggccccgc cggcttgatg ccggactcgt acggctccgt
    14941 ctcgggcccg tggtagcgcc gtccgccgag cagccacgag agcgtgagga tcagcgccgc
    15001 gacgacgagc acgagggccg cgtacagcgc cgccggccac acctccacag ccaaaaggcc
    15061 tccgctcacg cccaccccca tcgcgccccg tagggacccc gtcgacgtcc tttacgtcac
    15121 cacgttccca tggtagagaa caatcagtat caagcgtagt cgcctgtgtc catgcaatca
    15181 tcgtcgacgc cgtctcgtcg actcatgctg taccctgtgc ggtgaaagcg cagccggggc
    15241 gaagagaggg gagtccaggg gagtgagccg accgccggtc cagccgccga tgtggggccg
    15301 agaacccggc ctgtcgtggc ccgcgccggc gttgctcgtc ctcgcgctgg tcgccgccgt
    15361 gctcgcctgc gtggccttcg ccgcggccgg cgtctccgta tccgccgccg cggcctccgc
    15421 ccccccgccg ctccagtcgc cgcccgccga ggtcctgcct ccgcagggct ccggatcgtt
    15481 cacaccggcg gcgacgccgc agccggtgcc gtcgtccacc tatccgccgg gtaccggcgg
    15541 gagggtctac ggcgagaact gctccggctg ccacggcgcc cgcggcgagg gcctcgtcgg
    15601 tcctcccctg gcggcggcgg ccttcgccag cctggtcggg ccgatggtcg tggagggcgg
    15661 catcagcatg ccggcgttcg ccggggtcct gagcgacgcg gacgtggacg ccgtgtccga
    15721 cttcgtcgcc ggcgagctct ccgatccgga ggcccggacc gcgcaggtca cgccgggcgg
    15781 cgacctcttc cgtgtctact gctccggctg ccacagcgcc atcggcagcg gcggcgcgat
    15841 gccgtccctc aacaacgcgc ccaacatccg ccagtacccg cccgccgagg cgatcgcggc
    15901 gatgatcctc gggcgcggca acatgccgtc gttcgccggc aacaccctcg acgtgcgcca
    15961 gcaggcgtcc gtcgcgctgt acgtgcaggt gatcgacgac ccgccctcgc cgggcgggtt
    16021 cgggctgggg tatctcgggc cggtccccga gggcgccgcc ggcgccgtcg cgctgctcct
    16081 cctcatcctc atctccgtct ggctcgcctg gcgctcgcga aaggccgtcc cgtgacaccc
    16141 ccgcgcgtga ccacctggcc cgtcgggctc ggcatcgtcg ccgccatcgc cggcgcggtc
    16201 tccttcgccg tctgcttcgt gatcggcgcg agtaacgcct ggctcggcgg ctcgctcgcc
    16261 ctggcccttc tcggcctggg tctcgcgctc gcgtactggg gtcgcgacct ggccgacgac
    16321 cacgtcatcg cgggccggta ccccgtgccg cccgacgacc ccgagggtgg agccgcgctg
    16381 gccacccagc tcgacgagca cgcgtcggtc atcacgagga ggagcttcct cagcaaggcg
    16441 ctcgtgctcg gcatcggcgt gttcgcgctc agccaggtgg tgctgctcgg cgcgctcggc
    16501 cccttgccgg gcgacagcct ccgcaccacg ggctggcgca agggccgccg gctggtggac
    16561 tccgagggag agcccgtcac ccgggacgtg ctcgcgagcg gcgggttcct cgtcgccttc
    16621 cccgaggacg gcgaccacct ggagcgggcc gactcccaga tcgtcctgct gcacttcgtg
    16681 aacggtgagt tcacccctca gcccgggcgc gagacgtgga gccccgagga cttcgtcgcc
    16741 tactcgcgcg tctgcaccca cgccggctgc tccgtggccc agtacgccga cgtggaccag
    16801 ttgctgctgt gcccgtgcca ccagtcgacg ttcgacgtcc tcaacggcgc cgcgcccacc
    16861 agcggcccgg cgagccgccc gctgccgcaa ctgccgctgg ccatcgacga cgagggcgtg
    16921 ctgtacgcgc agagtgactt caccgagccc atcggccccg gcttctggaa cgctgtatga
    16981 gcaatcacga cctcaagcgg ggactgggca aggcgttcgg ggtgcgcacc tctccgctgc
    17041 gcccggcggg gcgctggctg cacaccaacc tcggcggcag ccgcctgctg cgcgaggagc
    17101 tgcgccacat cttcccggac agctgggcgt tcttcctcgg cgagatcgcc atgtactgct
    17161 tcgtcatcct ggtcgcgacc gggatcttcc tcgcgctctt cttccaggcg tcggaggcgc
    17221 cggtgatcta cgagggctcc tacgagccgc tcgcgggcgc cgagatgtcc atcgcctacg
    17281 actcggtgct gcagctgagc ctggacgtgc ccgccggcct gctcatgcga cagctgcacc
    17341 actgggcggc gctggtcttc gtcggcgccc tcatcatcca catgatccgc atgttcgtga
    17401 cggccgcgta ccggaggccg aggcgcatca actggctgat cggcctcagc ctggtgctcc
    17461 tcgtcggcat gaacgggctc atgggctact cgctgccgga cgacctgctc tcgggcaccg
    17521 gcctgcgcat ctcgttcgcg atcacggagt cgatcccgtt cgtcggcccg gcgttggcga
    17581 cgctgctgtt cggcggcgag ttcccgtccg gcgggttgat cgcccgcgtg tacccggtgc
    17641 acatcttcct gctgccggcg gcgatcggcg gcctgctggg cgcgcacctg gggctgctct
    17701 ggctgcagcg gcacacgcag ttcccgggtc cggggcgcag cgaccgcgtc atcgtcggca
    17761 cgccgatggt gccggcctac gcgctgcgca ccaccggcta cctgctgctg gtcgccgggg
    17821 tgtgcaccgc cttcgccgcc ttcgtgcaga tcaaccccgt ctggctctac ggcccgtacc
    17881 gcgcctggga cgcgaccacg gcggcgcagc cggactggta catgggctgg ctcgagggcg
    17941 gggtgcgcat gatgcccggc tgggacatcc agatcggcgg cctgctcatc ccggcgctgt
    18001 tctggccggc ggtggtgctg ccgggcctca tcttcacgcc gctgttcctg gtgccgtgga
    18061 tcgactgggt cgcgaaccgg gacggcgcct tccacaacgt gctgctgctg cccggccagc
    18121 accccgcgcg caccgcgctg gcggccgggt tcatcacgtt cctctcgctg ctgctggtgg
    18181 ccggcggcaa cgacgtcttc gcgttcgcgt acggctggtc acagccctgg ctcctcgagg
    18241 tcctccagtg gctgaccatc gtgctgccgc cggtcgtggc ggtcgtgacc tacgcgctgc
    18301 ttcgcggcgg ggcccggcgg tgggcgtcga cggtgcgctg aacgccgcgg ccgtcgcggc
    18361 cggccgctca gaggagctgg tcggcgacgg ccgccgccag cacgagcgcg aggcacggcc
    18421 ccgtcgcctt gaagagcgcc cacgcggcgc cgcccgccgg ccgcccgagc aggcgcgcgg
    18481 tgaacgccca cagcaccacg gcggcggcga ctgcgccggc cgcgtagacg gcgccgaaga
    18541 ggccggcgac gagcggcgcc gccagcacca ccgtcagcac ggcgacgaag ccggcgatca
    18601 ggcggcgggt ggcctcctcg ccgtagcgcg cgggcggcgt ggggatgccc gaggcgcggt
    18661 aatcgtcgcc gagggcgagc gtgagcgccc acgagtgcgg cggcgaccag accgggatga
    18721 gcacgcagag gaagaggatc gcgccggacc aggccgcgcc ggtcgccgcc cagccgatca
    18781 gggcggggaa gacgccggcc accccgccgg gcacggcgct cagcgcggtg cgcggcttga
    18841 gcagcagcgt gtagaccacc acgtagtaaa aggcgccgag cagcgcgaag acggcggcga
    18901 ggccgccggc gacggcccag agtgcagcgg ccccggcggc cacggcggcc aggccggcga
    18961 gcgcgccgcg cgccggcgcg agacggccgg aggccgtggg gcgccggcgc gtccgcgcca
    19021 tgctccggtc cacgtcgcgc tcgatgacgt tgttgagcag ggccgcgccg ccgacggtga
    19081 gcgcggtggc cgcgagcacc acgagcgcgc gccacgcggc cgggctgccg cagagcagca
    19141 tcgcggccag cacggtgacg aggaagagcg cgacgatacg cggctgcgcc gccctgaggt
    19201 agtccatggc cgccattcta gtaggtggac ctgcggcgcc ccgccagatg acccggctgc
    19261 ggcggtcatt gccgcatcgc cgtcgattcg tgggatgata ggcgcgcagc gccgcagggg
    19321 cggcaccgga cgagggggag aagtgcccgg gagcgtcaag gaggagcgtc gcgtgtcgcg
    19381 ggggccggcc gtggccgccg gcgcgttcgc cgtggtgctg gccgccctgg tcctcggcgg
    19441 ctgcgactcg ccggacatcc tgcgctcgga gggctcggcc ggcaacgaga tcgtcacgct
    19501 ggccaagtgg atcttcggca tcctcgcctt cgtcctgatc accgtctggg gcatcctctt
    19561 cttcgtcatc gtcacgggca aagggaggcg cgagcaggac gcctcgcaga ccaagggcaa
    19621 cctgcgcatc gagatcgtct ggacggccat ccccgcggtg atcgtcaccg tgctgttcat
    19681 cctgaccgtc gtgatcacca accgcatcga catgccggcc aagggcgcgc agttcacggc
    19741 gataggccgg cagtggtggt gggacttcga ctacaaggac ctgggcttca agtcgcccaa
    19801 cgagatctac gcgccggtgg gccgcccgat ctacatcgac gttgagtccg ccgacgtgat
    19861 ccactcgttc tgggtgccgc agatgggcgg caagaccgac atgatcccgg ggcacgtgaa
    19921 ccacgtccgc ttcgtacccc tcaagaccgg tgagtacctc ggcgagtgct ccgagttctg
    19981 cggggtccag cacggcaaga tgcgcttcct gttcgtggtc gtggagccgg aggagtacac
    20041 cgcctgggtc gagcggcagc agcagccggc ggccaaggcg acgggccctg aggccgtcgc
    20101 cggcgagaag gtcatcgcca gcgtgggctg cggcagctgc cacgccatcc gcggcacgac
    20161 gctcaagggc accttcgggc cggacctcac gcacttcggc agccgcaggg gcatcggcgc
    20221 ctacacgctg cgcaacacgc ccgagaacct gttgcgctgg atccaggaca cgcaggccgt
    20281 caagcccgac agcaacatgc ccaacgtgcc gctgccgctg gtgcagcagc agcagctgac
    20341 cgcctacctc ggagagctca agtgagcctc cggcgggcca tcgaccgggg cgccgcaggg
    20401 gcgccgctca ccggtgcggc gaactcgccg gccgccgccc acgccgagcg cggcggcctc
    20461 ggcgcctggc tcaccacgac ggaccacaag aagatcggtc tcatgtacat cggcacggcg
    20521 ctggtgttct tcctcatcgc tgtgatcttc gcgatgctca tgcgcacgca gctcatcaag
    20581 ccggagatgg ggctgctctc gccgcagacg tacaacgaga tcttctccat gcacggcacg
    20641 acgatggtgt tcctcttcgg catgccgatc ctcatcggcc tcgccaacta cctggtcccg
    20701 ctgcagatcg gcgcgcgcga catggcgttc ccgcgcgcca acgcactcag ctactggctg
    20761 ctgctgttcg gcgggctcgt cctgtacgcc agcttcctct tcggcggcgc gctggacacg
    20821 ggctggttca gctacgcgcc gctgacgctc aaggcgtact cgccgcacga cggcgtcacc
    20881 ttctggatcg tctcgctggt gctgctcggg ggctcgtcga tcctcggctc catcaacttc
    20941 atcgtcacgt gcctgcgctt ccgcgccaag ggcatgggcc tctggcagat gccgatcttc
    21001 tccgtggcca ccttcatcaa ctcgttcctc atcctcttcg ccatccccag cctgagcgcc
    21061 gcgctcgcgc tgctctacct cgaccgccag ttcggcacca gcttcttcga cccgaccggc
    21121 ggcggcgacc cgatcatctg gcagcacctc ttctggttct tcggccaccc ggaggtgtac
    21181 atcctcatcc tcccggcctt cggcatcatg agcgaggtcg tgccggcgtt ctcgcgcaag
    21241 cccctgttcg ggcgcagctc catggtcgtg atgctgggcg tgatcggcct cctcgggttc
    21301 ctggtgtggg cgcaccacat gttcgcggcg gggctgccga gcctcttcaa cacgatcatg
    21361 gccggcacca gcatgctgat cgcgatcccc acgggggtga agatcttcaa ctggctggcg
    21421 acgatgtggg gcggcgccct gcgcttcacg acctcgctgc tctacgcctg cggcctcatc
    21481 gccctgttca ccgtgggcgg catcaccggc gtcacgctgg ccgtggtgcc gtgggactgg
    21541 caggtgacgg acacctacta cgtcgtcgcc cacttccaca acgtgctgct ggccggcacg
    21601 gtgttcgcgg ccttcgccgg catctactac tggttcccca agatgaccgg ccggttcctc
    21661 tccgagcgca tcggcaaggc gcagttctgg ctcaccgcct tcggcttcgc gctcaccttc
    21721 ttccctctgt acgcgctcgg gatcatgggc atgccgcgga gggtgtacac ctacgcgccg
    21781 gacgtcggct ggaacgagtt gaacctggtc tcgtccatcg gcggctacat cctcgccctc
    21841 gggttcgtgc tgctcttcgt caacatcatc aggagcctgc gctccggcga gccggccggc
    21901 gagaacccgt ggcgggcctg gacgctcgag tgggcgacca cctcaccgcc tcagcacggc
    21961 aacttcgccc gcctgccgga gatccgcagc gaccggccgc tctgggacgc ggagaacggg
    22021 tacgaggacg agggcgggcg tgtcgaggag atcgcctggg tggacgtccc ggcggagccg
    22081 gagcagcgca cgatcacgcc gttctgggtg gccttcggga tcctcatcct cggcatcggc
    22141 ctgctgggcg tgccactggt gttcatcgtc ggcaccgcgc tcatcctgat cacactcgcc
    22201 gtgtggatga acgggcgctg gacggagatc gacccgacgc cgccgccgac gccggacgcc
    22261 gagcaccgct tctccttcat cggcctcggc acgctggcct tcatcggctc cgagtcggtg
    22321 ttcttcgcct cgctcatcgc ggcggtgatc cacctgcgca tccacaactc ggtgttcggc
    22381 ggcggcagcg gcgtggagat cacgctgccg ctcatcaaca ccgtcatcct cttcgccagc
    22441 ggcgtcaccg cgcactacgc gcagacggcc taccgagcgc ggcgcaaggg cggcttcttc
    22501 ttcctgctcg tgctcaccat cgtgctgggc gtgatcttcc tcggcggcca ggcctgggag
    22561 tactggcacg ccgggttcgg cctcagcgac tcgatcatcg cctcctcgtt ctacgtgctc
    22621 acgggcttcc acggcttcca cgtcctgtgc ggcatcctgg cgctcgtcta cctcctcttc
    22681 cgcgcgcgcc gcgagctgcg ccggcggccc ggagaacggg acctgccgga gcccacgccc
    22741 ggcacctccg ggctggtgga cggcggcacc tactactggc acttcgtcga cgccgtgtgg
    22801 gtggtcgtgt tcgtcgtcgt ctacctcctc tgagccccgg aggcgcagcg tgaggcgcgg
    22861 gctgggacgg cggctgaggc ggcggcgcgt gccgccggcc ggcgtgctgg cgttcctgct
    22921 gttcgcggcg gcggtgggcg cggcggccct gccggcagcc gccgccgcgc actctgcgct
    22981 cgagagctcc gaccccgccg cggatgcgtc cgtccccgtc tcgccgcggc agatcgtgct
    23041 cacgttctcc gaggccccgg acgccgagct ctcgctgatc cgggtcctcg acgcccaagg
    23101 cgcggcggtg cccggcatgt ccgcgccgca ggtcgcgccg ggcgcgtcca ccgcgctgca
    23161 ggtgacgccg tccgagccgc tggccgacgg cgtctacacg gtcaactggc gcgtggtctc
    23221 gagcgtggac ggccacgtgg agtccggcgc gttcgccttc ggtgtcggcg agcagcccgc
    23281 gcccgacagc gccatcgtcg tcgaactgct gcacacctcg ccgtgggcca ccgcgctcgc
    23341 cggtctgggt cgctggctgc tctacgcagg cctcgccctg ctcgtcggcg ccgcgaccac
    23401 cagcctgatc gtctacggcg gccggctgcc ccgcggcggc gtcaccgtgc tgcgcgccgc
    23461 cgccgccgtg gccgtcgcag gtctcgtcgc catggtgtgg tcgcagaagg tgctgatcgg
    23521 ggcgccgagc ctgctgccgc tgttcgtcat gcgtcagggg ctgctgctgc tggggctcgg
    23581 cgtcgccctc gtcctgtgca tcggcgccgt cgtcctcgcc gacctgtggc cggcccgctg
    23641 gagcctctgg ctcctcgggg gagccggcgc cgcggcggtc ctcctgcacg tcctggctgg
    23701 ccacgcggcg tccccgtcgt cgttccagtg gctgaacctg ctcgcccagt gggtgcacat
    23761 gacggcgatc ggcgtctggg tcggcgggct cttctggctg ctgctcgggt tccgcggccg
    23821 ggatgccgcg gagcgggggg cggcggtcgg tgtcttcacg cgcctggcga cggccgtgct
    23881 cggcgtcgtg ctgctgacgg gcctcgcgcg cgcggccgcg gaggtgggct cggtgagcgg
    23941 cttctacgac acgcgctacg gcatcacgct gctggtcaag atcgcgctgg tcgccgggct
    24001 cgtcgggctc ggcgccctca accatttctt ctgggcgccg ggggtgcgcg ccgcctcggg
    24061 cggcgcggcg gaccggcgct tcggcctcac ctcacgcggc gagctcggcg tggcgctcgc
    24121 cgtcctcgcc gtgacggccg tgctcagcgg ggtggtgccg gcgcgcctcg ccgcggccga
    24181 ccccgcgacg agcggcggct ccaccggcca gatctccgcc tccggcagcg actacgccac
    24241 ctccgtgcgc gtggaactca cgctcacccc gggggtcgcc ggccgcaacg actacgtgct
    24301 ctgggcggac gactacgaca ccggcgaccc gctcgagacc gtcaccgccg tgagcctgaa
    24361 gtgtgcgctg cccgcgcgcc cgtcggtcgc gcccgtgacg gtggaattga ccaaggctcc
    24421 cgacggcagc tggaccggcg gcggcctgga cttctccgtg gccggccgct ggcaggtcga
    24481 cgtcgccatc caggaggagg ccaagggctc ggtggtcccg ctcgagctca ccgtcccggc
    24541 ggcgtcctct ccctgaggcc gccggcgccg gcggggagac cgcgcggctc caggccgccg
    24601 cccgtttcga ccggcgccgc cccgggcagg ttcgcctcga cccgccggca cgggtcgtca
    24661 cgcaagggag ggaacatgag acgttccgcg acggcggtcc tgaccaccgc cctgatgctc
    24721 gtcctgctcg gcgtcctcgc ggcccctgtg ctcgcgcacg agacggcgtc cgccggaggg
    24781 gtgaatctcg agctcggatg gggcacggag ccggcctacc aggggcagct gaacaccatc
    24841 cagctcatcg tcacgcacaa ggccgacggc gacccggtca acgaccccgg cgccaggctg
    24901 acgaccacgg tcacctacgg cgaccaggag caggagttcg ccctgacgcc gacctacgac
    24961 ccggactacg gcacgggcac cccgggcgag tacgccgcgc tggtgatccc gaccgccccg
    25021 ggcgactaca cgttccacgt caccggcacc gtggagggcg ccaaggtgga cctcaaggtg
    25081 acgagctctc cgaagacgtt ctcaccggtg gaggacgcgt cggcggtgca gttcccggtc
    25141 aaggtcccgg cgaccgacca ggtggccgag cggctcgaca aggaactgcc gcggatggcg
    25201 gcggcggccg aggtggcgga tatggagagc caggtctcgt ccgcgacgac gctcgggtac
    25261 gtgggcatcg ccgtgggggc cgtcggcatc gtgctggccg ccgtggcgct gctgatgcgg
    25321 cggcgcgcct gaggccggcg cgcctgaagt ccttcgggct ggcacgccgg ccggccggcg
    25381 gctgggccgc ggcccctcag accgactggc cggagcctcc gctgaggtcg atcacgacct
    25441 ggccgtcgct gccgtagccg gtgagcttgt agacgccgcc gtccgcgctg acgtcgtagc
    25501 ggtagttgcc cgcgccgccg ccgtcggcca tcggcagatt gttgtagggg ttgaccggcc
    25561 aggccgggat gtacgcgcgc atgcccaccg cgttgacctc cccggccggc gggaactgcc
    25621 cgtcgtggtc ggcggcgtag gactcgatgc ccgcatggat ggtctgcacg ccggaccgga
    25681 cgaccgcgtc gttgccgaag ctctccgtgg tgcgcacgat gaacgcgggc agcaccgcgc
    25741 ccaggatggc gctgaggacc acgaaggcga cggcgacgac gatgaggacg atggcccagg
    25801 cgggcaggcc gcggcgcccg gatgcgcccg cgccgccggc aggagcggcc cccgccgtcg
    25861 ccggctgccc gccggtctcc cagcggtgga cctcggcgac ccaggcgcga agctcgggct
    25921 gggcccagac gagctgcccg agccggtcgt agtccagcac gccggcggcg gggatggcgc
    25981 cgtggccgac caggatgaac cggtccacgt cccagatgat ctcgcggccc tgccacgcgg
    26041 tggtggggcc gaacgtcgcg acgagcatgc tcccctcctc ggagtcgtcc gagcctagca
    26101 cgccgcgttc agcgtgcgtg aggagaaggc gtagcctcgc cgagcgcgtc gagcgcgggg
    26161 atgatgccca gcgcggccgc gctcatggcg tcgaaggcgt gcggcgacgg gagccgcgac
    26221 tccagcaggg ccgcgacgac ggccgtcgcg gcacgggtcg cgtcgagggt cgcccctgcg
    26281 agcgcgagct ctgcctcggg cgcgtagtac ccgcggtctt tgccggccgc gaggaaggcg
    26341 acgcgggcgc gcatgaacgc gtcgatcacg tccaggcgct cggccgcctt gcgcaggagg
    26401 cgctcgtgcc acggcgagat cgcggtgtcc gcgtcgggcg aagcctggag gggcccgtcg
    26461 tgcgccgtga ggtaggcgac gatcgagagg agttcggcgg cgcagcgctc ggcgtccggc
    26521 gggtacgtgg cggaggcgtg ggtctgcggc cgggactgcc ccgggtcacg ctgcagccgt
    26581 cctgtggcgc ccacgtccat ccaccctccc tcagaggcaa gcttgcaccc ggcggcccct
    26641 gtgcgcaagg ccggccatga gcttgactac tcagacgtga gcgtgagcgt gacgtccctg
    26701 gggagcgcga ggtcggtgat cacgtacatg gccttgaccg cgccgaccgg caccacgcag
    26761 gcctcgcagc agcccgccgt gcgcagcagc tctcgcgcct gcgcgaggac cgggttctcc
    26821 tcccggctca gcgactggat cgcgtcgacg ggcgagatct ccgcgatccg ccgggcgaat
    26881 tccgcgagga gctcgcactc ggactcgaag ctgaactcga cgtgcctggc gtcctcggtc
    26941 cttgcggtga cgacaacggg gtagccgcag atgccggggt ctatcgtctc tcgaagctcc
    27001 atgggtctct ctttcggcag ggcgcggtga tcccgctcag gccaccggca cgaaccggtc
    27061 gcccgcccgg gggaacagga ggttgttctc gaggtggacg tgtttgtgga ggtcggcctc
    27121 gaacgcctgc aggcgttcgt aggtgagcga ccaggtggcg caggcgtcgg ggggaagtcc
    27181 gtagcccatg gccaggtccc ggatatggtg cagggctgtg ccgacctctt cgtgctcgcg
    27241 gacgagcgcc tcgatgccgc gcgcgatcgt ctcggcatcc gccgcatcgg gagccgcgcc
    27301 ggtcctgccc gcggcctcgg ctcgcttgat ggccgggaag accactgctt cctcttgctc
    27361 gaggtgggcc gtcagctcgg tcgccatctg gtcgaacgcg gccacgatct cggcgagctc
    27421 ggggtgctgg gcgccgtgca cgtccgcgat cttgcgggtg taggcggcgg tctgggcgac
    27481 gttcgccctc acgtaggcgt ggtggatggc cttgatgtgg tcgatgaggc gcgtcagggt
    27541 ccagttcccg tagtccgcgt ccggccgcgc gggagtcctc gtcgccgcct cgagctcgcg
    27601 cagaagcacc gacgggtcga cgcggtgagc ggcacacgcg gcggccaggg tcacggcccc
    27661 gccgcagcag aagtcgatcc cgtgcctctc gaagaccgcg gccgtgcggt agtcgccggc
    27721 gacgatgtcg ccgaccgggg acgtctcggg tgtggggtct tcgtcgcgca cgctcgtctc
    27781 ctcgatggtg acgtgaagga cacctctcac catccccggc gggagcggag cggcaatctt
    27841 gaccttggtc aacgtccgag cgcaggcagt cggcggaacg cccgctcacc gtgtgttgcg
    27901 atgagcgggc gttgcctcag agctccccgt gagagggcga cctcacatcg gcgtccagcc
    27961 ggagaaaccc ttgtgcattc ctggggtcat gtcggcaccg agatgaccat cctgcgccat
    28021 cgcagcgtgg tgctggccga aggcctcgcc ggggtcgccg gaggcgttgg cagccagtgc
    28081 gctgcttggg acgacgagga gcgcgcctgc ggcgaggatc gtgacggcga acttcttcaa
    28141 aggaaatcct cctctggttg cgtttcgggt cgtgcgagtc gcgacccgac gtccaggcga
    28201 gaatgtaacc gcccgatgtg gaggtcctgt gaagaccgtg agagctgcgc gcgatggcgg
    28261 caagcctcgc ggggatcgtc agctatatcg ccatggacgg gtcacgagca tgtagatgcc
    28321 ccagatcacg aacccgatga tgccgagcca gatgaagaac ctgaggttcg agaagaggat
    28381 gcccgccaag atgaacgcga gcagaaggcc cccgacg
//
LOCUS       DASUMX010000236        19275 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_372100, whole genome shotgun sequence.
ACCESSION   DASUMX010000236 DASUMX010000000
VERSION     DASUMX010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 19275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19275
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372100"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="VFZ86_11060"
     CDS             <1..549
                     /locus_tag="VFZ86_11060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX5642859.1"
                     /translation="LRRQLLRLRPPLGVYGVFGNHDHGDSKAPFVQPTDPAFVETCGV
                     RLLFNETVALTRAGAAVEIAGVDDTDGGRDDLPAVLAALERRPGGLRLLLSHHAAVIR
                     RTAPGDFHMTFAGDTHGGQICLPLPGRRILLSDLGAEFAEGAYDVGGRPLYVTRGVGT
                     SMLPFRAFCRPEVVVFRVEAGG"
     gene            546..887
                     /locus_tag="VFZ86_11065"
     CDS             546..887
                     /locus_tag="VFZ86_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642860.1"
                     /translation="MRHSSSAILLVVLLLAAVTAPALSGCGNADPFAGLYWEPSTARR
                     VEIRKEGDAYLLYYGAALRPFTATRDGDELRIAEPMGGQTIVRLGEAEGTLELETAGE
                     TTLLKRLQQHQ"
     gene            complement(897..2030)
                     /locus_tag="VFZ86_11070"
     CDS             complement(897..2030)
                     /locus_tag="VFZ86_11070"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HEX5642861.1"
                     /translation="MSPSLVSHVRAEHGTGLGASVGRLVEPFGGWAGIVRPGERVAVK
                     INLLRAAPPEKAVTTHPETLACVLRALKAAGAEPFVADSPGGPNGPAKVARAYKLSGI
                     AGVCAAEGVQIVDVEDDRAVLEAPEGRLYRSFPIGKAFLDADAIVQVGVLKTHQLMRL
                     TGGVKLTYGVIPGLAKAHLHVKAQRREDFADMLLDLHLAVRPRFTIIDAIVAMEGQGP
                     GSGTPRALGSLFAARDCLALDAALADRTAHERRHVYTVAASARRGLLDLEDPYTLAGD
                     PIEPETDFKPVRRDMQELLPPSLHRLARNLITARPRLVDPDACIRCNECAAICGTRAI
                     AMDPLPDYDDAACVRCFACTEVCPTAAIDNVSPRLVRLFARDR"
     gene            complement(2027..2614)
                     /locus_tag="VFZ86_11075"
     CDS             complement(2027..2614)
                     /locus_tag="VFZ86_11075"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5642862.1"
                     /translation="MAEKFDPKHAGKLENPERLIELPPAKLVELLRLTGAETVVDFGA
                     GTGMYAIPLAAAVPTGRVLAVDEQPELLDRLRAKLAAHPGAGVEPVLSQGGRAPLDDA
                     VADRMLIVNVLHHVDDDPDALAEIWRLLAPGGLLVAAEFARMDRPVGPPNDHVLSLDR
                     VRALLTGAGLVELAVYAPGTVGLYHNVIVAEKPRP"
     gene            complement(2631..2921)
                     /locus_tag="VFZ86_11080"
     CDS             complement(2631..2921)
                     /locus_tag="VFZ86_11080"
                     /inference="COORDINATES: protein motif:HMM:NF014624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEX5642863.1"
                     /translation="MEEYMDQDEAGRLINRLRRVEGQSRGLQRMIEQGRPCEEIFTQL
                     AATKAALDRVGVLLISLKMRECLNEEAGDAVASPEAVEKALEAFLKYARCVK"
     gene            complement(3019..3879)
                     /locus_tag="VFZ86_11085"
     CDS             complement(3019..3879)
                     /locus_tag="VFZ86_11085"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX5642864.1"
                     /translation="MIDLHTHTTYSDGSQTPTELVEEAAAKGLSAVAVTDHDTVDGLP
                     EALAAGERLGVEVVPGVEINLEHDRVTMDMLGYFLAGMPTDELQRELKELRAYRVERN
                     DRMLERLADLGLPLSRTDLAAAGEDGAVGRPHIAEAMVRRGYAASISEAFELYLRRGA
                     PAWVDRRRLALGRALKLLRASGALPVLAHPGIIRTDAAGLNHIVRDAARAGLAGIECY
                     YPLHDAVTVARCLGLAEKYALVPTGGSDSHGTVKPEARLGVGSLGEPVPDEVLRDLRC
                     VAADQLQRLG"
     gene            3976..5010
                     /locus_tag="VFZ86_11090"
     CDS             3976..5010
                     /locus_tag="VFZ86_11090"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX5642865.1"
                     /translation="MRIGMIAPPWFPLPPQRYGGIEFVVSLLTEGLVARGHDVTLFAS
                     GDSETRARLSYIFARAPFEHIEKGAHLEVMHSIEAYKRAREFDVIHDHDGLASRAMGA
                     LVHELVGTPVVATLHGPADTITQEMLSSLRRDLRFIAISEYQRLGFPDLQFVGTIPNA
                     IDVEHMPFSAEKDDYLLFIGRMTADKGAHTAIDVARRLDQRLIMAGKVNEGPEREFFA
                     AEVEPYLSDKIHFRGEVDHDTKVELYKRARCTLFPIQWPEPFGLVMIESLACGTPVIA
                     MRQGSVPEVIEHGRSGFIVDTPDEMVAAFARIDEIDPAECRRAVEERFGSETFVAAHE
                     AAYAQLTAAG"
     gene            5016..6443
                     /locus_tag="VFZ86_11095"
     CDS             5016..6443
                     /locus_tag="VFZ86_11095"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX5642866.1"
                     /translation="MKLPYDDVHLIADPLYGYLRITLSRAGEIAEPDVIDNPWVQRLK
                     RIHQLQSSWWVFPTAEHSRFAHSLGTMHLAGKLARQVYPTLAEVEPGTPSLPLVEETL
                     RAAGLLHDLGHGPFSHFCDEEYLLPVFGIDHEQISQHLIVGELGPLLAELRRSPSGPF
                     AAGEAVDPWHVAFLVRAEEGAAATPPAAATPPSPDPIPRWVRLLHRALSGAVTVDNLD
                     YVRRDARMCGVGLGPIDVDRLVYYTFCTADGLTFHKRALSSLRAFLNARFYMYENVYF
                     HRIGHAIDLHLHEIFRPTLDLILPASPLEEPGAYRRLSEWSLFTTVEAWLDEPLHSTR
                     RALAEEWQSIIHRHVKYSMVYEHHVERQQPLPAARDLSREEFHAAIVRQLAPDLRGLE
                     FVVDIASQDPRPLNPFVGPKRLPVYDPATDRIEEQLLGGILEFVPAKAHLYRVFSVGR
                     EHERELALAAQAALGEIEPQSLTNL"
     gene            complement(6839..8245)
                     /gene="fumC"
                     /locus_tag="VFZ86_11100"
     CDS             complement(6839..8245)
                     /gene="fumC"
                     /locus_tag="VFZ86_11100"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEX5642867.1"
                     /translation="MDEGWRIEHDSMGEVRVPAEHYWGAQTQRSLEHFPIGTERFRWG
                     RRVIWALGVLKLAAARANEELGRLPQDKAELIARAAAEVMDGALDSEFPLVVFQTGSG
                     TQTNMNANEVIANRANELAGAGRGAGAPVHPNDDVNLGQSSNDTFPAAMHIAAAAELE
                     DGLMPAIEELRDTLAGKASEFEGVVMVGRTHLQDATPVTLGQVVSGWVAQLDQALTTV
                     RGTLPGLHELALGGTAVGTGLNTDPRFAETAAAHISSLTGKPFHPAANFFAALSAHDA
                     VVNASAALRTLAGALMKIANDVRWYASGPRDGLGELRIPENEPGSSIMPGKVNPTQAE
                     ALTQVAVRVFGNDTTVAFAGSQGNFQLNVYKPVMLHALLESAQLLAESARAFDTFCAR
                     GIEPDLEVIRRHLDASLMLVTALTPHIGYEKAAAVAEKAHREGLSLKDAAVGLGHVTA
                     EEFDAWVRPADMTHPLDA"
     gene            8369..8827
                     /locus_tag="VFZ86_11105"
     CDS             8369..8827
                     /locus_tag="VFZ86_11105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642868.1"
                     /translation="MKMRLLTGVMDPDQVAWARWLHDVMSSWPAQLAILLMWAGLALL
                     LARHARREWEGGLRNPYLLGGLIAGGVFFVAQFLFASSLAGGVTWPAWPFVVEFLVLF
                     ALWLYAFVRWITRQRGGPGDDEARRRGAALDRVIEQDGRSATAGSPPPPA"
     gene            8873..9223
                     /locus_tag="VFZ86_11110"
     CDS             8873..9223
                     /locus_tag="VFZ86_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HEX5642869.1"
                     /translation="MSEASGPPRRLAREEKTIAAMIALYCRDHHATAAADAGEGLCGE
                     CAELLAYARLRLEKCRYGADKPTCANCETHCYKPAMRERVREVMRYSGPRMLRRHPVL
                     AVAHLADGRKTPAD"
     gene            complement(9224..10279)
                     /locus_tag="VFZ86_11115"
     CDS             complement(9224..10279)
                     /locus_tag="VFZ86_11115"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEX5642870.1"
                     /translation="MKRTRRRASWQAVWIKRAVVLALTGLGLYVVWPSLAAVFSSGPQ
                     LRQVNPLWGLPIVAAELASFACMWALLRLALDVKPWFPVATAQLAGNAFSRIVPGGAA
                     AGGALQFDMLRQAGIGSTWAATGVTAVSLISTATLLGLPVISIVSILITGASVERNLF
                     RVGSIAVVVCLGVMAGGAVLLFADRPLLWVGDVVQKARNRILRRRAPLEDLADRLVDE
                     RDLIRRVLGGGWWQALLYSAGNWLLDLTALLIALAAVGAQPRASVVLLAYVVAAFLGM
                     IPITPGGLGFVEAGLVGTLSLAGIGTDQALLATLVWRLAAYWMPIPLGAAAYLLAARK
                     YGRPQHVEADAEGRIVS"
     gene            complement(10341..11807)
                     /gene="cls"
                     /locus_tag="VFZ86_11120"
     CDS             complement(10341..11807)
                     /gene="cls"
                     /locus_tag="VFZ86_11120"
                     /inference="COORDINATES: protein motif:HMM:TIGR04265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEX5642871.1"
                     /translation="MSVFLSLLPAWLSTVFYIYWFVLIIMVIMDDREPSITLAWLFVL
                     LLLPFIGVVFYILFGRDWKVVARRRGWVQRLHDTEREEMAPVYERNAGAAERFRREWA
                     SDMAVPIQRAITSENVAALLPATSLELFNDGAHKFRRLKEDLAGARRFIHMQYFIWKQ
                     DRLTAELVPILLDRLEAGVEIRITYDWIGCITFPKAELRQLKRAGAQVEADVTDLLRI
                     NYRNHRKIVVIDGEVGYTGGMNIGQEYIDGGERFATWRDTHLRLTGQAVAALEKLFAA
                     RWFEHTEKTGGENLFAAEYMPAPDPDAVATGTLVEVAAQGVEDPWSAARRTHMVAIGQ
                     AEESVWIQSPYFVPDYSIYDVMINAALSGIDVRFMMTGIPDKPIALWAAQTYYRKLIE
                     AGARVFLYQAGFLHSKTIVVDRTVSAVGTMNMDQRSLKLHKEMMVWVFDRSFAGQVAD
                     AFEVDMTNCHEVTLEDVLGVGRLKQFRNQAARLFSNVL"
     gene            11931..12431
                     /locus_tag="VFZ86_11125"
     CDS             11931..12431
                     /locus_tag="VFZ86_11125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642872.1"
                     /translation="MAHDPMHLFVGLYETGEAAERDFDNVVALHRQGLVAAYDAALIV
                     RGEDGALRVERKKHSGHHVLTGLGIGALLGVLTPFVAIPFGLLGAGGGALVRHAEGSL
                     PKKDAEELGASLTGSEAALAVVSNKTDSGRAGQMLPGAARRIAHVLDVEQEDFAEALR
                     RAMDEE"
     gene            12530..12868
                     /locus_tag="VFZ86_11130"
     CDS             12530..12868
                     /locus_tag="VFZ86_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEX5642873.1"
                     /translation="MWTILIFIVIGMVAGWAASLLIRREKYPSDWGVLFLIGIAGSLI
                     AGIIVNLLLGEGFKIAPGGIIGSIAVACLLLWLYVRAQNRGRRQRRAAPRADGQHRER
                     KGGRKHHTKR"
     gene            12883..14262
                     /locus_tag="VFZ86_11135"
     CDS             12883..14262
                     /locus_tag="VFZ86_11135"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX5642874.1"
                     /translation="MSRVLIVVANREDFPEPAFPVGALYVARAVEAAGGEARVFDAGG
                     YRRPLPALCAELRAWRPDTVGLSLRNADNASWPATLTYAGWYARVAATVREAAPQARL
                     VLGGPAFSIFPREMRRALLVADGVAGDGEVAARLIVEGRLPAGICERPLEDLAGGGLP
                     DDLGAVFPGAVRYRTAGVQTARGCPHGCVYCTYPGLEGAHLRRRPPEAVADEMERLRR
                     EVGATEQFIVDSSFNADEGHMTAVCEELRRRRSRPGAPLADVSFSCYLQPRLGDPGVF
                     GLLRAAGCTSVDFGIDTAAESLLPGLGKSFSIAGLRASTAAAKAAGIDVCHSLLFGGP
                     GETPATVAESVRVTDEVAPTAVVAMVGLRIYPGTELAGLAREAGLIGERQDLLEPRFY
                     AAGRLAGDPEVAWLPRQVREAAAARRSWFLPGARDWSAAWGPRLLRRFGKAGPLWRNF
                     PRPRWYRYV"
     gene            complement(14253..15056)
                     /locus_tag="VFZ86_11140"
     CDS             complement(14253..15056)
                     /locus_tag="VFZ86_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015725883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5642875.1"
                     /translation="MSHHTISVRDLGFAYPDGTRALDDVSFEVGHGEAIAVVGANGAG
                     KSTLLLHLNGLLTPERGSIDIGGIPVTKGTLPDIRRTVGMVFQDPDDQLFMPTVAEDV
                     GFGPLNLGLTPDDVNARVAAALARVGAEHLAERPPYHLSGGEKRSVAIATVLAMEPSI
                     LVMDEPSAGLDPRARRRLIGLLQGFEHTRIVATHDLDLAAQLCERTIVMGGGRLLADR
                     PTVAVFSDDALLEAGHLERPLGMLPCPVCGAAPEDGGSAPPGAAAARQT"
     gene            complement(15073..15885)
                     /gene="cbiQ"
                     /locus_tag="VFZ86_11145"
     CDS             complement(15073..15885)
                     /gene="cbiQ"
                     /locus_tag="VFZ86_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HEX5642876.1"
                     /translation="MSSVATGLSALDRLDRLARLDTPVHRVDPRAKVITTAVYIVCVV
                     SYGKYDLLGLVPFAVFPVVLATEGDLPLGFLGRLLLAVSPFALVMGAFNPLFDREVVA
                     QVGAWGVTGGWVSYASIVLRFLLTAGAALVLTATTSFTGICLALQKLKVPDVLVTQLL
                     LLFRYIFVLGDEASRMGQARRLRSFGRRGMGWRVHGQLLGQLLLRTFARAQRIYLAMK
                     ARGFDGEIRVARRLHFRASDLWFTLGWSAAFIALRAVNVPLALGHLVTGAVP"
     gene            complement(15898..16314)
                     /gene="nikR"
                     /locus_tag="VFZ86_11150"
     CDS             complement(15898..16314)
                     /gene="nikR"
                     /locus_tag="VFZ86_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HEX5642877.1"
                     /translation="MSDLVRFGVSIDERLLDRFDELITEKAYVNRSEALRDLIRGALV
                     EDQWEAGDAEAIGTVTLVYDHHSHDLADKLTDHQHTHHEEIVSTLHVHLDATYCLEVV
                     VLRGVAREIKRIADGLIGTKGVKHGRFVATTTGEIA"
     gene            16538..17602
                     /locus_tag="VFZ86_11155"
     CDS             16538..17602
                     /locus_tag="VFZ86_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015044799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HEX5642878.1"
                     /translation="MHMADALISPAVGGAMWAATGVVVAYSARKVRNDLDESKIPLMG
                     VAGAFVFAAQMLNFAIPGTGSSGHLGGALMLAILLGPEAAFLVMASILGVQALFFADG
                     GLLALGANIFNLGFFPAFIAYPLIYRRIVGDGPELKRGRIFAGSLLAAVVGLGLGAFA
                     VVLETTASGVTELPFSTFLLLMQPIHLAIGVVEGLVTAAVVLFVHGAQPELLARTATG
                     ASLGGVRLKPVIIGLGVAALVAGVALSWFAATSSDGLEWSIARATGSAEELQTSSSAH
                     DTAADLQDRTALLPDYGLAAEDTAAEPAAGEDAPAWPAVDAGTSLSGLVGGAVVAGVV
                     VLMGFLLHRRAARRRARAPG"
     gene            17682..18638
                     /locus_tag="VFZ86_11160"
     CDS             17682..18638
                     /locus_tag="VFZ86_11160"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX5642879.1"
                     /translation="MTGGAGFLGAAVVRRLVDRGDQVVGLVRTDEAATKLTRLGAVPV
                     RGDVTDARTLDQLLTDVDLVFHIAGDYRIGIREAEHEVMFKTNVDGTTLVLDAAVSAG
                     VQRIVYVSTVGVFGNTRGKVVDETYERPDRDFLSYYDATKYMAHRVAVGRQAAGAPLV
                     IAQPGAIYGPGDRSELGAQIDQAARGKYTIRAFSELGFTVGYIDDVADGVVLTQEKGA
                     AGEAYVLGGEVTRLGTVLDTVADLTGHKRARIKLPSTVFSAARPLGGLVGRLAGTGPN
                     LHEVVRASKDVTYWASSAKAERELGYAPRGLKQGLRDLLAAR"
     gene            complement(18666..>19275)
                     /locus_tag="VFZ86_11165"
     CDS             complement(18666..>19275)
                     /locus_tag="VFZ86_11165"
                     /inference="COORDINATES: protein motif:HMM:NF025734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heme-binding domain-containing protein"
                     /protein_id="HEX5642880.1"
                     /translation="PYGRSSHSNPPATNPFQWTDPQAEAIARESCYDCHSNETEWWWA
                     TNIAPFSWLVQRDVDGGRGEFNFSEWDGQPSVEAIAEAINGAMPPFQYTLIHPGAKLT
                     DAEKRTLLEGYAAGMAASGGQTEATPSATPSATPTSGSSAEAVAIINQRCSISTCHTP
                     DRALAFRASSAAEAQALLDDMIRRGAQVTTEEQQALVQYFTQ"
BASE COUNT         2686 a         6804 c         7013 g         2772 t
ORIGIN      
        1 ctccgccggc agctgctccg cctgcgcccg ccgctcggcg tctacggcgt gttcggcaac
       61 cacgaccacg gcgacagcaa ggcgcccttc gtgcagccga cggacccggc cttcgtcgag
      121 acctgtggcg tccgcctgct cttcaacgag acggtggccc tgacgcgcgc cggcgcggcg
      181 gtggagatcg ccggcgtcga cgacaccgac ggcggccgcg acgacctgcc ggccgtgctc
      241 gccgcgctcg agcgccggcc cggcggcctg cgcttgctgc tctcgcacca cgccgcagtg
      301 atcaggcgga cggcgccggg ggacttccac atgaccttcg ccggcgacac ccacggcggc
      361 cagatctgcc tgccgctgcc gggccgccgc atcctgctca gcgacctcgg cgccgagttc
      421 gccgagggcg cctacgacgt cggcggccgg ccgctctacg tcacgcgcgg cgtgggcacg
      481 agcatgctgc cgttccgcgc cttctgccgc ccggaggtcg tggtgttccg cgtggaggcc
      541 ggcggatgag acactcgtcc tcggccatcc tcctggtcgt gctcctgctg gcggcggtca
      601 cggcgccggc gctgtccggc tgcgggaacg ccgacccctt cgccgggctc tactgggagc
      661 cgtcgaccgc gcgtcgcgtc gagatccgca aggagggcga cgcctacctg ctctactacg
      721 gagcggcgct gcggccgttc acggcgacgc gggacggcga cgagctccgc atcgcggagc
      781 cgatgggcgg tcagaccatc gtgcgcctcg gcgaggccga gggcacgctc gagctggaga
      841 cggcgggcga gaccacgctg ctcaagcggc tgcagcagca ccagtagcgg cccgcgtcag
      901 cggtcgcggg cgaagaggcg gacgaggcgc ggcgagacgt tgtcgatggc cgccgtgggg
      961 cagacctcgg tgcacgcgaa gcagcgcacg caggccgcgt cgtcgtagtc ggggagaggg
     1021 tccatggcga tggcgcgggt gccgcagatg gcggcgcact cgttgcagcg gatgcaggcg
     1081 tccggatcca ccagccgcgg acgcgcggtg atcaggttgc gcgccagccg gtgcagcgac
     1141 ggcggcagca gctcctgcat gtcccggcgc accggcttga agtcggtctc gggctcgatg
     1201 ggatcgccgg cgagggtgta cgggtcctcg aggtcgagga ggccgcggcg tgcggaggcg
     1261 gcgaccgtgt agacgtggcg gcgctcgtgg gcggtgcggt cggcgagcgc cgcgtccagc
     1321 gccaggcagt cgcgggccgc gaagagcgag ccgagcgcgc gcggcgtgcc gctccccggc
     1381 ccctggccct ccatggcgac gatcgcgtcg atgatggtga accgcgggcg cacggcgagg
     1441 tggaggtcga ggagcatgtc cgcgaagtcc tcccgccgct gggccttgac gtggagatgc
     1501 gccttggcca gccccgggat gacgccgtac gtgagcttga cgcccccggt gaggcgcatg
     1561 agctggtgcg tcttgaggac gccgacctgg acgatggcgt cggcgtccag gaacgccttg
     1621 ccgatgggga acgagcggta caggcgtccc tccggcgcct ccaggacggc gcgatcgtcc
     1681 tccacgtcca cgatctggac gccttcggcg gcacacacgc cggcgatgcc gctgagcttg
     1741 taggcgcgcg cgaccttggc ggggccgttg gggccgccgg ggctgtcggc gacgaaaggc
     1801 tcggccccgg ccgccttgag cgcgcgcagc acgcaggcga gcgtctccgg atgcgtggtg
     1861 acggccttct cgggcggcgc cgcgcggagc aggttgatct tgacggcgac gcgctccccc
     1921 ggccgcacga tcccggccca gccgccgaag ggctcgacga ggcggccgac cgacgcgccg
     1981 agccccgttc cgtgctcggc gcgcacgtgg gagaccagcg agggactcaa ggcctcggct
     2041 tctccgcgac gatcacgttg tggtacagac ccaccgtgcc gggcgcgtac accgcgagct
     2101 ccacgaggcc cgcaccggtg agcagcgccc gcacccgatc gagagaaagc acgtgatcgt
     2161 tgggcggccc gaccggccgg tccatgcgcg cgaactcggc ggccaccagc aggccgccgg
     2221 gcgccagcag ccgccagatc tcggccaggg cgtccgggtc gtcgtcgacg tggtgcagta
     2281 cgttgacgat gagcatgcga tcggccacgg cgtcgtccag cggcgccctg ccgccttggc
     2341 tcaggaccgg ctcgacgccg gcgccggggt gggcggccag cttcgcgcgc agacggtcga
     2401 gcagctcggg ctgctcgtcc acggcgagca cgcgcccggt ggggacggcg gcggccaagg
     2461 ggatggcgta catgccggtg cccgcaccga agtccacgac ggtctcggcg ccggtgaggc
     2521 ggagcagttc caccagcttt gccggcggca gctcgatgag cctctccggg ttctcgagct
     2581 tcccggcgtg ctttgggtcg aacttctcgg ccactgctgc gccgggaccg ctacttgacg
     2641 cagcgggcgt acttgagaaa ggcctcgagg gccttctcga cggcctccgg ggaggcgacg
     2701 gcgtcgccgg cctcctcgtt gaggcactcg cgcatcttca gcgagatgag cagcacgccc
     2761 acgcggtcga gcgccgcctt ggtggcggcg agctgggtga agatctcctc gcagggacgg
     2821 ccttgctcga tcatccgctg caggccacgg gactggccct cgacccggcg caggcggttg
     2881 atcaggcgcc ctgcctcgtc ctgatccatg tactcctcca cacgcacgac tatacccccg
     2941 ggggggatga gggacaacag atttcccggc ggaggctgcg ctacccggta cccacgcccc
     3001 gtaggggtat ccgaggcgtc agccgaggcg ctgcagctga tctgccgcca cgcagcgcag
     3061 gtccctcagc acctcgtccg ggaccggctc gcccagggag ccgacgccca ggcgagcctc
     3121 gggtttgacg gtcccgtgag agtcggagcc gccggtgggg accagggcgt acttctccgc
     3181 cagtccgagg cagcgcgcca ccgtcaccgc gtcgtgcagc gggtagtagc actcgatgcc
     3241 ggcgaggccg gcgcgcgcgg cgtcccgcac gatgtggttc agccccgcgg cgtccgtgcg
     3301 gatgatgccg gggtgcgcga gcacggggag cgcgcccgag gcgcgcagca gcttgagggc
     3361 gcgccccagc gcgagccgcc gtctgtccac ccaggcgggg gcgccgcggc gcaggtagag
     3421 ctcgaacgcc tcgctgatcg aggccgcgta cccgcggcgc accatcgcct cggcgatgtg
     3481 cgggcggccg acggcgccgt cctcaccggc ggcggcgagg tcggtccggc tgagcggcag
     3541 gccgagatcg gccaggcgct cgagcatccg gtcgttgcgc tcgacccggt acgcgcgcag
     3601 ctccttgagc tcgcgctgga gctcgtcggt cggcatgccc gcgaggaagt agccgagcat
     3661 gtccatggtc acccggtcgt gctcgaggtt gatctccacg cccgggacga cctccacgcc
     3721 gaggcgctcg ccggcggcca gcgcctcggg caggccgtcc actgtgtcgt ggtcggtgac
     3781 ggcgacggcc gacaggcctt tcgccgccgc ctcttccacg agctcggtgg gcgtctgcga
     3841 cccgtccgag tacgtcgtgt gcgtgtgcag gtcgatcatg cttcaaccct accccgacgg
     3901 cgcaggagct gcgctgggcc ccgccggggc cgggtatgat gcgtgtagtc aagaatgccc
     3961 ctctggaggt cagcgatgcg catcgggatg atcgcgccgc cctggttccc cctgccgccg
     4021 cagcggtacg gcggcatcga gttcgtcgtc agcctcctca cggagggcct cgtggcgcgc
     4081 ggtcacgacg tgacactgtt cgccagcggc gactcggaga cgcgggcgcg gctcagttac
     4141 atcttcgccc gcgccccctt cgagcacatc gagaagggcg cccacctcga ggtgatgcac
     4201 agcatcgagg cgtacaagcg cgcgcgcgag ttcgatgtca tccacgatca cgacgggctc
     4261 gccagccgcg cgatgggcgc actcgtccac gagctcgtcg gcacacccgt cgtggccacg
     4321 ctgcacgggc cggccgacac catcacccag gagatgctga gctcgctccg ccgggacctc
     4381 cggttcatcg cgatcagcga gtaccagcgg ctgggctttc ccgacctgca gttcgtcggc
     4441 accatcccca acgccatcga cgtggagcac atgccgttca gcgcggagaa ggacgactac
     4501 ctgctgttca tcggccgaat gaccgccgac aagggcgcgc acaccgccat cgacgtcgcc
     4561 cgccgcctgg accagcgcct catcatggcc ggcaaggtca acgagggccc cgagcgggag
     4621 ttcttcgccg cggaggtcga gccgtacctc tccgacaaga tccacttccg cggcgaggtc
     4681 gaccacgaca ccaaagtcga gctctacaag cgcgcgcgct gcacgctgtt ccccatccag
     4741 tggccggagc cgttcggcct cgtcatgatc gagtccctgg cctgcggcac gccggtgatc
     4801 gccatgcgcc agggctccgt gcccgaggtc atcgagcacg gccgcagcgg gttcatcgtc
     4861 gacacgccgg acgagatggt cgcggcgttc gcgcgcatcg acgagatcga ccctgccgag
     4921 tgccgtcgcg cggtggagga gcggttcggc agcgagacgt tcgtggccgc gcacgaggcg
     4981 gcgtacgcgc agctcaccgc ggcaggctga ggtccatgaa gctgccgtac gacgacgtcc
     5041 acctcatcgc cgacccgctg tacggctatc tgcgcatcac gctctcacgg gccggggaga
     5101 tcgccgagcc ggacgtcatc gacaacccct gggtgcagcg gctcaagcgc atccaccagc
     5161 ttcagagttc gtggtgggtg ttccccaccg cggagcacag ccggttcgcc cacagcctcg
     5221 ggaccatgca cctggcaggc aagctggcgc ggcaggtgta ccccaccctg gccgaggtcg
     5281 agccggggac gccgtccctg cctctggtcg aggagaccct gcgcgcggcc ggcctgctgc
     5341 acgacctcgg tcacggaccg ttcagccact tctgcgacga ggagtacctg ctccccgtct
     5401 tcgggatcga ccacgagcag atctcgcagc acctgatcgt cggcgagctg gggccgctgc
     5461 tggccgagct gcggcgcagt ccctcggggc cgttcgccgc gggcgaggcg gtggatccct
     5521 ggcacgtcgc ctttctcgtg cgcgcggagg agggcgccgc cgccaccccg ccggccgccg
     5581 ccacgccgcc ctcgccggac cccatcccgc gctgggtccg cctgctgcac cgcgcgctct
     5641 ccggagccgt cacggtcgac aacctcgact acgtgcgccg ggacgcgcgc atgtgcggcg
     5701 tcggcctcgg gccgatcgac gtcgaccggc tcgtctacta caccttctgc accgcggacg
     5761 gcctcacgtt ccacaagcgc gcgctgagct cgctgcgcgc ctttctcaac gcgcgcttct
     5821 acatgtacga gaacgtgtac ttccaccgca tcggccacgc catcgacctg cacctgcacg
     5881 agatcttccg gcccacgctc gacctcatcc tgccggccag tccgctcgag gagccgggag
     5941 cgtaccggcg gctcagcgag tggtcgctgt tcaccaccgt cgaggcatgg ctggacgagc
     6001 cgctgcacag cacgcggcgg gccctggcgg aggagtggca gagcatcatc caccgccacg
     6061 tcaagtacag catggtgtac gagcatcacg tcgaacggca gcagccgctg cccgcggcgc
     6121 gcgacctgag ccgcgaggag ttccacgccg ccatcgtccg gcagctggct cccgacctgc
     6181 gcggcctcga gttcgtggtc gacatcgcct cgcaggaccc gcggccgctc aatccgttcg
     6241 tcggaccgaa gcggctgccc gtgtacgacc ccgccaccga ccggatcgag gagcagctgc
     6301 tcggcggcat cctcgagttc gtccccgcca aggcccacct gtaccgggtg ttctcggtgg
     6361 gccgcgagca cgagcgtgag ctcgccctcg cggcccaggc ggcacttggc gagatcgagc
     6421 cgcagagcct caccaacctc tgaacggggt cgccggcgag cgcactgatc gtcggggtcc
     6481 ggcgtgaggt gatctgcccc ttccggggac tgccgcctga tctcttcgag gatatcaagg
     6541 ggcagcgccg cgcagggata ccctcaccct acggcaggcg catccgcggg acgggtgccg
     6601 gagggtcagc cgacgacgcg gacgatgacg agtacgaaga ccgcgatcat caccgcgagc
     6661 accacgagca gcgcgatgat cagggtgttg agcatgccgt cgcccgagcg ggcggaggag
     6721 gtcttcgtgg ccggccggcg ggccggcgcc ggccgcgagg cttggcgcgc tgcccgatca
     6781 cgctcgcgct gtgcggcgtt ccgctcgccg cgccgccggt ctggccgcga cttcgcgctc
     6841 acgcgtccag cgggtgggtc atgtctgcgg gccgcaccca cgcatcgaac tcctcggcgg
     6901 tcacgtggcc gaggccgacg gcggcatcct tgagcgacag gccttcgcgg tgcgccttct
     6961 cggcgaccgc ggccgccttc tcgtagccga tgtgcggcgt gagcgccgtc accagcatga
     7021 gcgaggcgtc gaggtgccgg cggatcacct cgaggtccgg ctcgatgccc cgcgcgcaga
     7081 aggtgtcgaa ggcgcgcgcg gactccgcca gcagttgcgc cgactcgagc agcgcgtgca
     7141 gcatcaccgg cttgtagacg ttgagctgaa agttgccctg cgagccggcg aaggcgacgg
     7201 tcgtgtcgtt gccgaacacg cgcacggcga cctgggtgag cgcctccgcc tgggtcggat
     7261 tgaccttgcc gggcatgatc gacgaacccg gctcgttctc ggggatgcgc agctcgccga
     7321 gcccgtcgcg cggacccgac gcgtaccagc gcacatcgtt ggcgatcttc atgagcgcgc
     7381 cggccagcgt gcgcagagcc gcgctcgcgt tgacgaccgc gtcgtgcgcc gagagcgcgg
     7441 cgaagaagtt ggcggccggg tggaacggct tgccggtgag gctactgatg tgggccgccg
     7501 cggtctcggc gaagcggggg tcggtgttca gcccggtccc gaccgccgtg ccgccgaggg
     7561 ccagctcgtg caggccgggc agcgtgccgc ggaccgtcgt cagcgcctgg tcgagctggg
     7621 ccacccagcc cgagacgacc tggcccagcg tcaccggcgt cgcgtcctgc aggtgcgtgc
     7681 ggccgaccat gaccacgccc tcgaactccg acgccttgcc ggcgagcgtg tcgcgcagct
     7741 cctcgatcgc cggcatcagc ccgtcctcga gctcggcggc ggcggcgatg tgcatcgccg
     7801 cggggaaggt gtcgttgctc gactgcccca gattgacgtc gtcgttgggg tggacggggg
     7861 cgccggcgcc cctgcctgcg ccggcgagct cgttcgcgcg gttggcgatc acctcgttgg
     7921 cgttcatgtt ggtctgcgtg ccgctgccgg tctggaacac gaccaggggg aactctgagt
     7981 cgagcgcgcc gtccatgacc tcggcggcgg cgcgcgcgat gagctcggcc ttgtcctgcg
     8041 gcaggcggcc gagctcctcg ttggcccgcg ccgccgcgag cttcaggacg ccgagcgccc
     8101 agatcacgcg gcggccccag cggaaccgct ccgtgccgat ggggaagtgc tccagcgagc
     8161 gctgcgtctg cgcgccccag tagtgctcgg cggggacgcg cacctcgccc atgctgtcgt
     8221 gctcgatgcg ccagccctcg tccatgccgg taagactacc ccgtcaacgg cgggagatgt
     8281 ccagcggtgc cagccgctcg ccgcggagac cgcacggcgt atgcttgggt gacgggcggg
     8341 ccgcggtccg cacccacagg gaggacacgt gaagatgcgg ctcctgacag gcgtgatgga
     8401 tccggaccag gtggcttggg cgcggtggct gcacgatgtg atgtcgtcct ggccggcgca
     8461 gctggcgatc ctgctgatgt gggctgggct cgcgctgctc ctcgcgcgcc acgcgcggcg
     8521 cgagtgggag ggcggcctgc gcaatcccta cctcctcggg ggcctcatcg caggtggcgt
     8581 cttcttcgtg gcccagttcc tcttcgccag ttcgctggcg ggcggcgtga cctggccagc
     8641 gtggcccttc gtggtcgagt tcctcgtgct gttcgcgctg tggctgtacg ccttcgtgcg
     8701 ctggatcact cgccagcgcg gcggacccgg ggacgacgag gctcgcaggc gcggcgccgc
     8761 tctcgatcgg gtcatcgagc aggacggccg ctccgccacg gccgggtcac cgccgccgcc
     8821 cgcctgacca gagtcaaggc cgcttccgcc cgcgggcggg tatgagtggg tcatgagcga
     8881 agcgtccgga ccgccgcgcc gcctggcgcg tgaggagaag accatcgcgg cgatgatcgc
     8941 gctgtactgc cgcgaccatc acgcgaccgc ggctgccgat gcgggcgaag ggttgtgcgg
     9001 cgagtgcgcc gagctgctgg cctacgcgcg cctccgtctg gagaagtgcc gctacggcgc
     9061 ggacaagccc acctgcgcca actgcgagac gcactgctac aagcccgcga tgcgcgagcg
     9121 cgtgcgcgag gtgatgcgct acagcgggcc gaggatgctc cgtcgccacc cggtgcttgc
     9181 cgtcgctcac ctcgccgacg ggcgcaagac tcccgccgac tagctagctg acgatccgtc
     9241 cctccgcatc agcttcaacg tgctggggcc ggccgtattt gcgcgccgcg aggaggtacg
     9301 cggcggcgcc cagggggatg ggcatccagt aggcggcgag gcgccagacc agcgtcgcca
     9361 gcagcgcctg gtcggtgccg atgccggcga ggctgagcgt accgaccagg ccggcctcca
     9421 cgaaccccag gccgccgggc gtgatgggga tcatgccgag gaacgccgcg acgacgtagg
     9481 cgaggagcac gaccgacgcg cgcggttgcg cgcccacggc cgcgagcgcg atcagcagcg
     9541 ccgtgaggtc gagcagccag ttgccggcgc tgtagaggag cgcctgccac cagccgccgc
     9601 ccagcacgcg acggatgagg tcgcgctcat cgaccaggcg gtcggccagg tcctccagcg
     9661 gggcgcgcct gcgaaggatg cggttgcgcg ccttctggac cacgtctccg acccagagca
     9721 gcgggcggtc ggcgaacagc agcaccgccc ctccggccat caccccgagg cagaccacga
     9781 cggcgatcga gccgacgcgg aacaggttgc gctccaccga ggccccggtg atgaggatcg
     9841 agacgatgct gatcaccggc agaccgagca gcgtggccgt ggagatcagc gagaccgccg
     9901 tcacgccggt cgccgcccac gtgctgccga tgccggcttg gcgcagcatg tcgaactgca
     9961 aggcgccgcc tgcggcggcg ccccccggca cgatgcggct gaaggcgttg ccggccagct
    10021 gcgccgtcgc caccgggaac cacggcttga cgtcgagcgc caggcgcagc agcgcccaca
    10081 tgcaggcgaa gctggcgagc tccgccgcga cgatgggaag accccagagc gggttgacct
    10141 gtctgagctg cgggccggac gagaagaccg cggcgaggct cggccacacg acgtacaggc
    10201 cgagcccggt cagcgcgagc acgacggccc gcttgatcca gactgcctgc cagctcgccc
    10261 tgcggcgagt cctcttcacg gcgatcttcg gccgttcctg tggctcgggc tgagccaggg
    10321 tccctcctcg ggaaggatgg tcagagcacg ttcgagaaga gccgggccgc ctggttgcgg
    10381 aactgcttca ggcggcccac gccgagtacg tcctccagcg tcacctcgtg gcagttcgtc
    10441 atgtcgacct cgaacgcgtc ggcgacctgt ccggcgaagc tcctgtcgaa cacccacacc
    10501 atcatctctt tgtgcagctt gagactgcgc tggtccatgt tcatcgtacc cacggcgctc
    10561 accgtgcggt cgacgacgat cgtcttcgag tgcaggaagc cggcctggta gaggaagacg
    10621 cgggcgccgg cctcgatgag cttgcggtag taggtctgcg cggcccagag cgcgatcggt
    10681 ttgtccggga tgccggtcat catgaagcgc acgtcgatgc cgctgagagc ggcgttgatc
    10741 atcacgtcgt agatgctgta gtcggggacg aagtagggcg actggatcca gaccgactcc
    10801 tcggcctggc cgatcgcgac catgtgggtg cggcgggccg cgctccatgg gtcctcgacc
    10861 ccctgcgcgg ccacctcgac gagcgtcccg gtcgccaccg cgtccgggtc cggcgccggc
    10921 atgtactctg ccgcgaacag gttctcaccg cctgtcttct ccgtgtgctc gaaccaccgc
    10981 gccgcgaaca gcttctccag cgccgcgacg gcctggccgg tcaggcgcag atgcgtgtcg
    11041 cgccaggtgg cgaagcgctc gccgccgtcg atgtactcct gaccgatgtt catgccgccg
    11101 gtgtagccga cctcgccgtc gatcacgacg atcttgcggt ggttgcggta gttgatgcgc
    11161 agcaggtcgg tgacgtcggc ctccacctgg gcgccggccc gcttgagctg ccggagctcc
    11221 gccttgggga acgtgatgca gccgatccag tcgtacgtga tgcggatctc gacgccggcc
    11281 tcaagacggt cgagcaggat cggcaccagc tcggcggtga ggcggtcctg cttccagatg
    11341 aagtactgca tgtggatgaa gcgtctggcc ccggccagat cctccttgag ccgccggaac
    11401 ttgtgggcgc cgtcgttgaa caactcgagc gaagtggccg gcaggagcgc ggcgacgttc
    11461 tcgctcgtga tcgcccgctg gatgggtacg gccatgtcgc tcgcccactc gcggcggaac
    11521 cgttctgcgg cgccggcgtt gcgttcatag accggcgcca tctcctcccg ctcggtatcg
    11581 tgcagccgct ggacccagcc gcgccgccgc gcgacgacct tccagtcccg cccgaagagg
    11641 atgtagaaga cgacgccgat gaacggcagc aacagcagga cgaacagcca ggcgagcgtg
    11701 atggacggct cgcggtcgtc catgatcacc atgatgatca ggacgaacca gtagatgtag
    11761 aagaccgtgc tcagccacgc gggcaggagc gagaggaaga cggacatggc ggcacgctat
    11821 ccgaccgcga cggccgtgtc acgcagccgt gcgcgagctc ccgcgcccgg cgtggctcgc
    11881 ggtggcgcgc ccggggtagg gtggtgacga ccgacccgaa ggagggacac atggcgcacg
    11941 accccatgca cctgttcgta ggtctgtacg agaccggaga ggcggcggag cgcgacttcg
    12001 acaacgtcgt cgcgctgcac cggcagggcc tggtggccgc ctacgacgcg gcgctcatcg
    12061 tgcgtggcga ggacggcgcg ctgcgcgtcg aacgcaagaa gcacagcggg catcacgtcc
    12121 tgaccgggct cggcatcggg gcgctcctcg gcgtgctcac gccgttcgtc gccatcccct
    12181 tcgggctcct cggggccggc ggcggcgcgc tggtcaggca cgccgagggc agcctgccga
    12241 agaaggatgc cgaggagctc ggggcgtcgc tcacgggcag cgaggcggcc ctcgccgtgg
    12301 tcagcaacaa gaccgacagc ggacgcgcgg ggcagatgct ccccggggcg gcgcggcgca
    12361 tcgcccacgt gctggatgtg gagcaggagg acttcgccga ggccctgcgc cgggcgatgg
    12421 acgaggagtg aacccggcga tggcccgggc gtagcgagcg gcaggggctc cctccgcgat
    12481 agaatccgcg cgcccgtcgt cgcctatccg aacaggagca cccgcagtca tgtggaccat
    12541 cctcatcttc atcgtcatcg gcatggtcgc cgggtgggcg gccagcctgc tcatccgtcg
    12601 cgagaagtac cccagcgact ggggcgtcct gttcctcatc ggcatcgccg ggtcgctgat
    12661 cgccgggatc atcgtcaacc tcctcctggg cgaggggttc aagatcgccc cgggcgggat
    12721 catcggctcc atcgccgtcg cctgcctgct gctctggctc tacgtgcgcg cgcagaaccg
    12781 cggccggcgg cagcgccgcg ccgcaccgcg cgccgacggc cagcaccgcg agcgtaaggg
    12841 cggcaggaag caccacacca agcggtagtc catactgtac ggatgagccg cgtcctcatc
    12901 gtcgtcgcga accgcgagga cttccccgag cccgcctttc ccgtcggcgc gttgtacgtg
    12961 gcgcgcgccg tcgaggccgc cggcggcgag gcgcgcgtct tcgacgcggg cgggtaccgg
    13021 cggccgctcc cggcgttgtg tgcggagctg agggcgtggc ggccggacac cgtggggctc
    13081 tcgttgcgca acgccgacaa cgcctcctgg cccgccacgc tcacgtacgc gggctggtac
    13141 gcacgcgttg cggccaccgt gcgcgaggcc gcgccgcagg cgcggctcgt cctcggcggc
    13201 ccggcgttct cgatcttccc gcgggagatg cgccgtgcgc tgctcgtcgc cgacggcgtg
    13261 gccggcgacg gcgaggtggc ggctcgcctg atcgtggagg gccgcctgcc ggccggcatc
    13321 tgcgagcgcc cgctcgagga cctggccggg ggcggcctgc cggacgacct cggcgcggtg
    13381 ttccccggag ccgtccggta ccgcaccgcc ggcgtgcaga cggcgcgcgg ctgtccgcat
    13441 gggtgcgtct actgcaccta cccggggctc gagggcgctc acctgcggcg gcggcctccc
    13501 gaggcggtcg ccgacgagat ggagcggctg cggcgggagg tcggcgcgac cgagcagttc
    13561 atcgtcgact cctcgttcaa cgccgacgaa ggccacatga cggcggtgtg cgaagaactg
    13621 cgccggcggc gcagccggcc cggcgcgccg ctcgcggacg tctccttctc ctgctacctg
    13681 caaccccgcc tcggcgaccc gggcgtgttc ggcctgctcc gggcggcggg gtgcacctcc
    13741 gtggacttcg gtatcgacac cgccgccgag tcgcttctcc ccggcctcgg caagtcgttc
    13801 tcgatcgccg gcctgcgcgc ctcgacggcg gcggccaagg ccgccgggat cgacgtgtgc
    13861 cactcgctgc tgttcggcgg cccgggcgag acgcccgcca cggtcgcgga gagcgtgcgc
    13921 gtgaccgacg aggtggcgcc gaccgccgtg gtggcgatgg tcggcctgcg catctacccg
    13981 ggcacggagc tcgccgggct ggcccgcgag gcgggcctca tcggcgagcg ccaggacctg
    14041 ctggagccgc ggttctacgc cgcggggcgc ctcgccggcg acccggaggt ggcgtggctg
    14101 ccgcggcagg tgagagaggc ggcagccgcg cgccggagct ggttcctccc cggcgcgcgc
    14161 gactggagcg ccgcgtgggg accgcggctg ctgcggcggt tcggcaaggc agggccgctg
    14221 tggcgcaact tcccgcggcc ccgctggtac cgctacgtct gacgcgccgc cgcggcgccg
    14281 ggcggcgcgg atccaccgtc ctcgggcgcc gcgccgcaga cggggcaggg cagcatgccg
    14341 agcggccgct cgaggtggcc cgcctcgagc agcgcgtcgt cgctgaagac cgcgaccgtc
    14401 gggcggtccg ccagcaggcg gccgccgccc atcacgatgg tccgctcgca gagctgcgcg
    14461 gccaggtcga ggtcgtgcgt cgcgacgatg cgcgtgtgct cgaacccctg caggaggcct
    14521 atgaggcgcc gccgggcgcg cgggtcgagg ccggcggacg gctcgtccat caccaggatg
    14581 ctgggctcca tggccagcac cgtggcgatc gcgaccgagc gcttctcgcc gccggacagg
    14641 tggtagggcg ggcgctccgc gaggtgctcg gcgccgacgc gcgcgagcgc tgcggcgacg
    14701 cgggcgttga cgtcgtcggg cgtgaggccg agattgagcg gcccgaagcc gacgtcctcg
    14761 gcgacggtcg gcatgaacag ctggtcgtcc ggatcctgga acaccatgcc gacggtgcgg
    14821 cggatgtccg gaagggtccc cttggtcacc gggatgcctc cgatgtcgat gctgccgcgt
    14881 tcgggcgtga gcagcccgtt gaggtgcagc agcagcgtcg acttgccggc gccgttggcg
    14941 ccgacgaccg cgatcgcctc gccgtgaccg acctcgaagg agacgtcgtc cagcgcccgc
    15001 gtcccgtccg gataggcgaa gccgagatcg cggacgctga tggtgtggtg gctcacggga
    15061 cgctccgctc gctcacggga ccgctccggt gacgaggtgg cccagcgcca gcgggacgtt
    15121 gacggcgcgg agcgcgatga acgcggccga ccatcccagc gtgaaccaga ggtcgctcgc
    15181 gcgaaagtgc agccgccggg cgacccggat ctcaccgtcg aacccgcgcg ccttcatcgc
    15241 caggtagatg cgctgcgccc gggcgaaggt gcgcaggagc agctggccga gcagctggcc
    15301 gtggacgcgc cagcccatac cgcggcgtcc gaagctgcgc aggcgccgcg cctgtcccat
    15361 gcgcgaggcc tcgtcgccga gcacgaagat gtagcggaag agcaacagga gttgggtgac
    15421 gagcacgtcg gggaccttga gcttctgcag ggcgaggcag atgccggtga agctggtcgt
    15481 cgcggtgagc accagcgccg cgcccgccgt caacagaaag cgcagcacga tcgaggcgta
    15541 ggagacccag ccgccggtga cgccccaggc gccgacctgt gccacgacct cgcggtcgaa
    15601 gaggggattg aaggcgccca tgacgagagc gaagggggag accgcgagga gcaggcggcc
    15661 gaggaagccg aggggcagat cgccctccgt cgccaggacg acggggaaga ccgcgaacgg
    15721 cacgaggccc agcaggtcgt acttgccgta ggagacgacg cagacgatgt agacggcggt
    15781 cgtgatgacc ttcgcgcgcg ggtctacgcg gtggacgggc gtgtcgagcc gggcgagacg
    15841 gtcgagacgg tcgagggcgg agaggccggt ggcgacggag gacacgtgcc gccggcccta
    15901 cgcgatctcg cccgtcgtgg tcgccacgaa cctgccgtgc ttcacgccct tcgtgccgat
    15961 gagcccgtcg gcgatgcgct tgatctcccg cgcgacgccg cgcagcacca ccacctcgag
    16021 gcagtaggtg gcgtcgaggt gcacgtgcag cgtcgacacg atctcctcgt ggtgcgtgtg
    16081 ctggtggtcc gtgagcttgt cggcgaggtc gtgcgagtgg tgatcgtaga ccagggtcac
    16141 ggtcccgatg gcctcggcgt cacccgcctc ccactggtcc tcgacgagcg cgccgcggat
    16201 gaggtcgcgc agcgcctcgg agcggttcac gtaggctttc tcggtgatga gctcgtcgaa
    16261 gcggtcgagc aggcgctcgt cgatcgagac gccgaagcgg acgaggtcgg acacggcgcc
    16321 tctcccttcc cgcgatcgtg gcgtgccacg cgatgggcat cgggatggca cgcgtcgtac
    16381 catccccggc agaagaagtg gcaccatccg cgcacttctt gacgcccgtc gcaggctgcc
    16441 ctacagtgcg gtcctacagt agcacgagtt tgcagtccgt agcaccgaag cccgccgatc
    16501 ggcccgcgac ccactcagcc cgggaggaca cggccccgtg cacatggcag acgcgctcat
    16561 ctctcccgcc gtcggcggcg ccatgtgggc cgcgaccggt gtcgtcgtcg cctacagcgc
    16621 gcgcaaggtg cgcaacgacc tggacgagag caagatcccg ctgatgggcg tcgccggcgc
    16681 cttcgtcttc gccgcgcaga tgctcaactt cgccatcccc ggcacgggct cgagcggcca
    16741 cctcggaggg gcgctgatgc tcgccatctt gttggggccg gaggccgcct tcctggtgat
    16801 ggcctcgatc ctgggcgtcc aggcgctgtt cttcgccgac ggcgggctgc tggcgctcgg
    16861 cgccaacatc ttcaatctcg ggttcttccc ggcgttcatc gcctacccgc tcatctacag
    16921 gaggatcgtg ggggacggcc cggagctcaa gagggggcgg atcttcgccg gctctcttct
    16981 cgccgccgtc gtcggcctcg gtctgggcgc gttcgccgtc gtgctggaga cgaccgcctc
    17041 cggcgtcacc gagttgccgt tctcgacctt cttgctcctc atgcagccca tccacctggc
    17101 gatcggcgtg gtggaggggc tggtgacggc ggccgtggtc ctcttcgtgc acggggcgca
    17161 gcccgaactg ctggcgagga ccgcgacggg cgcgtcgttg ggcggcgtgc gcctcaagcc
    17221 ggtgatcatc ggcttggggg tggcggcgct ggtcgccggc gtcgcgctct catggttcgc
    17281 ggcgacgagc tccgacggtc tggaatggtc catcgccagg gccacgggct ccgcggagga
    17341 gctgcagacg agctcctcgg cacacgatac ggcagccgac ctgcaggacc gcaccgcgct
    17401 cctgccggac tacggcctgg ctgcggagga cacggcggcg gagcccgccg ccggggagga
    17461 cgcgccggcg tggccggcgg tcgacgccgg caccagtctc tccggcctcg tcggcggcgc
    17521 cgtcgtcgcc ggtgtggtcg tcctcatggg gttcctgctt caccgtcgcg ccgcgcgccg
    17581 gcgggctcgc gcgcccggct gacgggcgcg agcgctcgcc ccgcgtgcag gccggcgtgg
    17641 gctcgcgtcg aacgggtagg gacgcgcgta tgaaaagctt tgtgacaggt ggtgctgggt
    17701 tcctcggcgc ggcggtcgtc cgccgcctgg tcgaccgcgg tgatcaggtc gtcgggctgg
    17761 tgcgcacgga cgaggcggcc acgaagctga cccgcctcgg cgccgtcccc gtgcgcggcg
    17821 acgtcaccga cgcgcgcacc ctcgaccagc tgctcaccga cgtcgacctc gtgttccaca
    17881 tcgccggaga ctaccggatc ggcatcaggg aggcggagca cgaggtgatg ttcaagacca
    17941 atgtggacgg cacgactctc gtcctcgacg cggccgtctc cgccggggtg cagcggatcg
    18001 tctacgtctc caccgtcggc gtcttcggca acacccgcgg caaggtcgtg gacgagacct
    18061 acgagcggcc cgaccgagac ttcctcagct actacgacgc caccaagtac atggcgcacc
    18121 gggtggcggt ggggcggcaa gctgccgggg cgccgctggt gatcgcgcag cccggcgcca
    18181 tctacgggcc gggcgaccgc agcgagctcg gcgcccagat cgatcaggcg gcgcggggca
    18241 agtacaccat ccgcgccttc tccgagctgg ggttcaccgt gggctacatc gacgacgtcg
    18301 ccgacggcgt cgtgctcacc caggagaagg gcgccgccgg agaggcctac gtgctcggcg
    18361 gcgaggtgac ccgtctcggg acggtcctcg acacggtggc cgatctcacc gggcacaagc
    18421 gcgccaggat caagctgccc tcgacggtgt tctcggcggc acggccgctc ggtggcctcg
    18481 tcggccgcct cgccggcacc gggccgaacc tgcacgaggt cgtccgcgcc agcaaggacg
    18541 tcacctactg ggcctcgagc gccaaggcag agcgagaact ggggtacgcg ccgcgcggac
    18601 tcaagcaggg gctccgcgac ctcctcgcgg cgcgctgacc caggggcgcg ggacgcgccc
    18661 cggcgtcact gcgtgaagta ctggaccagc gcctgctgct cctcggtcgt cacctgcgcc
    18721 ccgcgccgga tcatgtcgtc gagcagtgcc tgcgcctcgg cggcgctcga ggcgcggaac
    18781 gcgagcgctc tgtcgggagt gtggcaggtg gagatgctgc agcgctggtt gatgatcgcc
    18841 acggcctcgg cgctggagcc ggaggtcggc gtcgccgagg gcgtcgccga gggcgtcgcc
    18901 tcagtctggc cgccgctcgc cgccatgccg gccgcgtaac cctccagcag cgtgcgcttc
    18961 tcggcgtcgg tcagcttggc gccggggtgg atgagcgtgt actggaaggg cggcatcgca
    19021 ccgttgatgg cctccgcgat cgcctccacc gatggctgcc cgtcccattc ggagaagttg
    19081 aactcccccc ggccgccgtc gacgtcgcgc tggaccagcc aggagaacgg ggcgatgttg
    19141 gtcgcccacc accactcggt ctcgttgctg tggcagtcgt agcaggactc cctggcgatg
    19201 gcctcggcct gtgggtcggt ccactggaag gggttggtgg ccgggggatt ggagtggctg
    19261 ctgcgcccgt agggc
//
LOCUS       DASUMX010000237         2959 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_946729, whole genome shotgun sequence.
ACCESSION   DASUMX010000237 DASUMX010000000
VERSION     DASUMX010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_946729"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="VFZ86_11170"
     CDS             <1..459
                     /locus_tag="VFZ86_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642881.1"
                     /translation="LILTDVPRAYVDYGTPEQTALERVTASQMRAYAAAGHFKAGSMG
                     PKVEACLRFVDAGGESVIASLTEVGPAMAGRAGTHIVPDGEGRRASEPAGGEDAASGK
                     PGAKPQAKAAGAKKGAARKQPAKRPRRSTEAAAGADVIDIATARRRTAAR"
     gene            488..1582
                     /gene="aguA"
                     /locus_tag="VFZ86_11175"
     CDS             488..1582
                     /gene="aguA"
                     /locus_tag="VFZ86_11175"
                     /EC_number="3.5.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047632 - agmatine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase"
                     /protein_id="HEX5642882.1"
                     /translation="MTTTIDSTPRADGFRMPGEFEPHRQIWMLWPQRPDNWRLGAKPA
                     QRAWVEVASAISRFEPVTVGVDHDQYENAVDLLPDAVRVIELSSNDAWMRDCGPTFVV
                     NDQGDVRLVDWDFNAWGGLHDGLYFPWDKDQLVPQKLAQVEGVDRYKARIVMEGGSIH
                     VDGEGTVITTEECLLSPGRNPEMTRAQIEEHLRDYLGVEKVIWLAKGIDPEETNGHVD
                     DVACFVRPGVVLAAVTEDEDDWRYELLQDNLRVLRAATDARGRGLEVHTVPMPAIMEI
                     TDEEAWGVDSAEGSIPRRPGDKTAASYLNFLIVNGGVILPAFDDPNDGVAREIIAALF
                     PEREVVTVPGREIVLGGGNVHCITQQQPRG"
     gene            complement(1622..2083)
                     /locus_tag="VFZ86_11180"
     CDS             complement(1622..2083)
                     /locus_tag="VFZ86_11180"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5642883.1"
                     /translation="MKPRDDTVTITEFSMDDYAVVHVLWQRGDLWMRPSDGPEATRLK
                     LTRDPGLFLVARSPDGRVVGTVMGGWDGRRAYVYHLAVVPERRREGIADALMDELEER
                     FRKLGALKAKLQILVENETSKAFFARRGYLLETDCEPWGKELVEGGAPPSR"
     gene            2249..2779
                     /locus_tag="VFZ86_11185"
     CDS             2249..2779
                     /locus_tag="VFZ86_11185"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX5642884.1"
                     /translation="MDALDLMLARRSIRRFTSQDVSREDEQRLIDAAFAAPSANNDRP
                     WHFVCVRDPEMRRRLRGMHEWTRMLDKAPLVIAVLGRAEGHPWWIEDCSAATENILLE
                     AASLGLGAVWCGMREDPDAVAGYERACCEALGAPHDQWRALALIAVGHPAEQKKPRTQ
                     RQASKLSYERFGRRSR"
BASE COUNT          460 a         1061 c         1057 g          381 t
ORIGIN      
        1 ctcatcctca ccgacgtgcc ccgggcctac gtcgactacg gcacccccga gcagaccgcg
       61 ctcgagcgcg tcaccgccag ccagatgcgc gcctacgccg ccgccggcca cttcaaggcc
      121 ggctccatgg gccccaaggt cgaggcctgc ctgcgcttcg tcgacgccgg cggcgagagc
      181 gtcatcgcca gtctcaccga ggtcggcccg gcgatggccg gcagggcggg gacgcacatc
      241 gtgcccgacg gggagggacg gcgggcttcc gagcccgcag gaggcgagga cgccgcctcc
      301 ggcaagcccg gcgccaaacc gcaggcgaag gccgccggcg cgaagaaggg ggcggcccgc
      361 aagcagcccg ccaagcgtcc gcgccgctcc accgaggcgg ccgccggcgc cgacgtcatc
      421 gacatcgcca ccgcccggcg gcggaccgcc gcccggtgac ggccccagcg gtcatcagga
      481 ggtccccatg accacgacca tcgacagcac gccccgcgcc gacggcttcc gcatgcccgg
      541 ggagttcgag ccgcacaggc agatctggat gctgtggccg cagcgtcccg acaactggcg
      601 actcggcgcg aagccggcgc agcgcgcctg ggtggaggtc gcctcggcga tctcgcgctt
      661 cgagcccgtg acggtcgggg tggaccatga ccagtacgag aacgcggtgg acctcctccc
      721 cgacgccgtg cgcgtgatcg agctctccag caacgacgcc tggatgcgcg actgcggccc
      781 gaccttcgtc gtcaacgacc agggtgacgt ccgcctcgtc gactgggact tcaacgcctg
      841 gggcgggctc cacgacggct tgtacttccc ctgggacaag gaccagctgg tgccgcagaa
      901 gctcgcccag gtggagggcg tcgaccgcta caaggcccgc atcgtgatgg agggcggctc
      961 gatccacgtg gacggcgagg gcacggtcat caccaccgag gagtgcttgc tgagtcccgg
     1021 gcgcaacccg gagatgacgc gcgcgcagat cgaggagcac ctgcgtgact acctgggcgt
     1081 cgagaaggtg atctggctgg cgaagggcat cgaccccgag gagaccaacg gccacgtgga
     1141 cgacgtcgcc tgcttcgtgc gaccgggtgt cgtgctggcc gccgtcaccg aggacgagga
     1201 cgactggcgc tacgagctcc tgcaggacaa cctgcgcgtg ctgcgcgcgg ccaccgacgc
     1261 ccgagggcgc ggcctcgagg tgcacaccgt accgatgccg gcgatcatgg agatcaccga
     1321 cgaggaggcc tggggcgtcg acagcgccga gggctcgatc ccgcggcggc ccggcgacaa
     1381 gaccgccgcc tcgtacctca acttcctcat cgtcaacggc ggcgtgatct tgccggcgtt
     1441 cgacgacccg aacgacgggg tggcccgcga gatcatcgcc gcgctgttcc cggagcgcga
     1501 ggtcgtcacg gtgcccggcc gggagatcgt gctcggcggc ggcaacgtgc actgcatcac
     1561 gcagcagcag ccgcgcggct gaggcgagcc tcgcctccct cacggggagg cccggggctc
     1621 atcacctgga gggcggcgcg ccgccctcca cgagctcttt gccccacggc tcgcagtcgg
     1681 tctccagcag gtacccgcgg cgggcgaaga aggccttgga ggtctcgttc tcgacgagga
     1741 tctgcagctt ggccttgagc gcgccgagct tgcggaagcg ctcctcgagc tcgtccatga
     1801 gcgcgtcggc gataccctcg cggcggcgct ccgggaccac cgcgaggtgg tagacgtagg
     1861 cgcggcggcc gtcccagccc cccatcaccg tgcccaccac cctgccgtcc ggggagcgcg
     1921 cgaccaggaa gagaccgggg tcgcgcgtca gcttgaggcg cgtcgcctcg ggcccgtccg
     1981 acggccgcat ccacagatcg ccgcgttgcc acagcacgtg gaccacggcg tagtcgtcca
     2041 tgctgaactc ggtgatggtg acggtgtcgt cgcgcggctt catgcggtct ccttggggcg
     2101 cgattgtacc gcgcgtggcc ggcgacgaca gccgaacccg cggccggcga tccttgcgct
     2161 gtcggtcgcc tcggagggcc ggagcatgga tgcgcacggc tcgcgggtac aatcggctca
     2221 gccacagccg agtacgggag ggagcgtgat ggacgcactc gacctcatgc tggcacgacg
     2281 cagcatccgt cgcttcacat cgcaggacgt gtcgcgcgaa gacgagcaga ggctgatcga
     2341 cgcggcattc gctgccccga gcgcgaacaa cgaccgcccc tggcacttcg tctgtgtccg
     2401 cgacccggag atgcgccggc ggctcagggg catgcacgag tggacgcgga tgctcgacaa
     2461 ggcgccgctc gtcatcgccg tcctgggtcg cgcggaaggc cacccctggt ggatcgagga
     2521 ttgctccgcc gccaccgaga acatcctcct cgaggcggca tcgctcgggc tgggcgccgt
     2581 ctggtgcggg atgcgggagg acccggacgc ggtcgccgga tacgagcgcg cctgctgtga
     2641 ggccctcggc gcgcctcacg accagtggcg cgcgctcgcc ctcatcgccg taggtcaccc
     2701 cgccgagcag aagaagccgc gcacgcagcg ccaggcgagc aagctcagct acgagcgttt
     2761 cggccggcgc tcgcgctgac gggaggccgc cgtgagcgcc gtccccagca ccgaggaccg
     2821 catccaggac acggcgcggt ccatcttcgc cgccatggcc ggcgacaagc ccgggcggct
     2881 cagccgcgag cgcctgcagg gtgaggtgat ggagtgggcg atggcggacg agcatctcaa
     2941 ggtcgagatg ctgcggttc
//
LOCUS       DASUMX010000238         2178 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_228379, whole genome shotgun sequence.
ACCESSION   DASUMX010000238 DASUMX010000000
VERSION     DASUMX010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228379"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="VFZ86_11190"
     CDS             complement(<1..296)
                     /locus_tag="VFZ86_11190"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX5642885.1"
                     /translation="MQAVVIREVGAFELADLPAPEPGTGEALIRVSVTGLCRTDLKIV
                     RHGHRDLTLPRVPGEEVVGEIVRLGPHDTSAPQGSEPFAPGDRVYVYPGVWCGE"
     regulatory      complement(418..612)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            660..1157
                     /locus_tag="VFZ86_11195"
     CDS             660..1157
                     /locus_tag="VFZ86_11195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642886.1"
                     /translation="METRPGTIVNVMSLVVLVLCLALSWSPWYVAGTLGYEPEIVVSY
                     PAARSVAYWAAAVLLAVACAAAFVASRHGARRRAAWRGLSLAASAGACSSVVFGLFVE
                     PGLATRLRGTSFAPVALVTADPQALLASGVDTFFMSPGYGLWAVLATAIAGVVTAIVG
                     LWARD"
     gene            1266..>2178
                     /locus_tag="VFZ86_11200"
     CDS             1266..>2178
                     /locus_tag="VFZ86_11200"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HEX5642887.1"
                     /translation="MAREPLTRAEFLKILAAGAGGVVFLGGSEDALAALAGADDDLDE
                     AALNGDAGRAAALPYMTVVHGTPVDVNVRTAIAKLGGMRRFVSAGDDVVIKPNICAAR
                     AAKYAATTNPIVVATLVRLARGAGARRVRVMDNPISSSPSSCYSASGIAAAVKAAGGS
                     MQVMSASRYVTYDIPGGTLGRLPLYRDMLACDVLINVPIAKQHGSTGLTLSGKNLMGC
                     TSNRGKMHNLGLSRSIAELNAKLKPDLTVLDAVRILVRNGPTGGSLSDVRRKDVVIAS
                     KDWVAVDAYATRLFGKTPRFVPYIAAAA"
BASE COUNT          289 a          824 c          741 g          324 t
ORIGIN      
        1 cactcgccgc accagacgcc cgggtagacg tagacgcggt cccccggcgc gaacggctcg
       61 gagccttgag gagcggacgt gtcgtgcggc ccgaggcgca cgatctcacc caccacttcc
      121 tctcccggca cgcgcggcag cgtgaggtcg cggtgcccgt gccggacgat cttgaggtcg
      181 gtgcggcaga ggccggtgac gctcacccgg atgagggcct cgcctgttcc gggctcgggc
      241 gccggcagat cggccagctc gaacgccccg acctcacgga tcaccactgc ctgcatcgcg
      301 ccgcctttcg gtctccaaac gcacagaagc ccgggccgtc gcactcgtct ggcccgggcc
      361 ccccgttttc gaggccgcag cgcgcgtcgc cgccggcccg ttcaccacgc gggtcgcgtc
      421 gacagcgcac ctggcggcag gtcttctggc tcccggatcg tcctcgggcc gcgccttccc
      481 gccggttccc cggcagtggc ttctcgcagc cttcgtcccc ggtcacagcg gcgggtccgc
      541 gccggtcttg caccggcttc cctcgagtgg ccctcgcggg cgcctccaag cgccgcggat
      601 ggtacgcgag agccggcgac ggggtcaagc cacgaggccg gggcgataga ctccggcagg
      661 tggagactcg tcccggaacg atcgtcaacg tgatgtcgct cgtcgtcctc gtcctttgcc
      721 tggctctgtc ctggagcccc tggtacgtgg ccggcacgct cgggtacgag cccgagatcg
      781 tcgtctcgta cccggctgcg aggtccgtcg cgtactgggc ggctgcagtc ctgctggcgg
      841 tcgcctgcgc cgcggccttc gtcgcctccc ggcacggcgc ccggaggcgc gccgcgtggc
      901 gcggcctctc gctcgcggcc agcgccggag catgctcgag cgttgtgttc ggactcttcg
      961 tcgaaccggg tctggcgact cggctccggg gaacctcctt tgccccggtc gccctcgtca
     1021 ccgccgatcc tcaggccctc ctggcgagcg gcgtcgacac gttcttcatg tcgcccggct
     1081 atggcctctg ggcggtgctc gcgacagcga tcgccggcgt ggtgacggcg atcgtcgggc
     1141 tctgggccag agactgacga cccgtcgacg cgcgtcgtct ccccgccggc gacgccggcg
     1201 tctcattctg gcagtagcgc cgccggggag taccatggac gcgacggagc accagggggt
     1261 ggaccatggc acgcgaaccg ctcacccgcg ccgagttcct caagatcctc gccgccggcg
     1321 ccggaggcgt cgtcttcctc gggggctcgg aggacgcgct ggccgcgctc gccggcgccg
     1381 acgacgatct ggacgaggcg gcgctcaacg gcgacgccgg ccgcgccgcg gcgctcccct
     1441 acatgacggt cgtgcacggc acccccgtcg acgtcaacgt caggacggcg atcgccaagc
     1501 tgggtgggat gcgccgcttc gtctcggccg gcgacgacgt ggtcatcaag cccaacatct
     1561 gcgccgcgcg ggcggcgaag tacgcggcga ccaccaaccc catcgtggtc gccaccctcg
     1621 tgcgcctcgc ccgcggcgcc ggcgcccgca gggtccgcgt gatggacaac cccatctcgt
     1681 ccagcccgag cagctgctac tcggccagcg ggatcgccgc ggcggtcaag gctgccggcg
     1741 gctcgatgca ggtgatgagc gcctcccgct acgtgaccta cgacatcccc ggaggcaccc
     1801 tcgggcgcct cccgctctac cgggacatgc tcgcctgcga tgtgctcatc aacgtcccga
     1861 tcgccaagca gcacggttcg accggcctca cgctgagcgg caagaacctg atggggtgca
     1921 ccagcaaccg cggcaagatg cacaacctcg ggctgagccg ctcgatcgcc gagctgaacg
     1981 ccaagctcaa gccggacctc accgtactgg acgccgtcag gatcctggtg cgcaacggcc
     2041 ccaccggcgg cagcctgagt gacgtgaggc gtaaggacgt cgtgatcgcg agcaaggact
     2101 gggtcgcggt cgacgcctac gcgacgcggc tgttcggcaa gacgccgcgc ttcgtgccgt
     2161 acatcgccgc cgccgcca
//
LOCUS       DASUMX010000239        25331 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_87008, whole genome shotgun sequence.
ACCESSION   DASUMX010000239 DASUMX010000000
VERSION     DASUMX010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 25331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25331
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87008"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..827)
                     /locus_tag="VFZ86_11205"
     CDS             complement(6..827)
                     /locus_tag="VFZ86_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEX5642888.1"
                     /translation="MRVACVQLNTRDDVAANVRAAVDLVEAAADRGARLVALPETWAF
                     KGPREGIVATAEAPDGPSNRALAAVAARRGVWLLAGSVYEPAPGGLVANVSALFGPTG
                     DLRAAYRKIHLFDVTTATAQYQESDEVVPGEEVVTADIDTVDGAAVRLGLSICYDLRF
                     PGLYSSLALRGARILCVPAAFTAYTGAAHWEVLLRARAIENGCFVVAPDQVGEHLPGR
                     DCFGHSMIVDPWGEVLARLDDGVGLCVADLDLARLEAVRGQVPSLSHRRPDVYDC"
     gene            complement(875..1828)
                     /gene="speB"
                     /locus_tag="VFZ86_11210"
     CDS             complement(875..1828)
                     /gene="speB"
                     /locus_tag="VFZ86_11210"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEX5642889.1"
                     /translation="MKGYGPDGGARSPRIAGVTTFLRLPHVTQFDDVDVAVVGLPFDT
                     GLAVRTGARFGPRAVREASLTIHPAYNPAQRVAVFERLSVVDAGDVRAVAGGFIDRSL
                     AAMEETLGQVHGAGAVPLGVGGDHTVTLAELRAAAAKHGPVALLHFGAHTDCLDEGAG
                     ERHIHSTVIRRAQEEGVIDASRSALLGMRGGLDSPDEYEQARELGFTVVPWDDLAQLG
                     TGVVAAAVEAIGDAKAFITVDVDFVDPGFAPAVGTPEVGGPTSAQALALIRGCRGLDL
                     AGADVVEVVPELDSSHLTATVAATIAYELLSLMACSGGTCE"
     gene            1983..3641
                     /locus_tag="VFZ86_11215"
     CDS             1983..3641
                     /locus_tag="VFZ86_11215"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX5642890.1"
                     /translation="MPDERLRILVIDDDGDLTATVAAVLTANGYEVLTATTGEQGLEL
                     AFTGNPHLILLDVTMPGMDGHQVCRELQFGYTKDIPVVFFTARTELAHMMEANRSGAS
                     AYITKPFRPDHLLATITNVLRDSSVYHDDVTGLPTLANVQLEVQRMLFDHGQLGIIYV
                     SIGGVQALEQLQGFEVVDLVYRVVGQGLENARGSLIRGDDFVSISSLGDAFLIVLSPA
                     RKNGFISEEDLLLVKERLEKELLDRLENDLESRLLTSVDLFVGCSRLTQSPKIRFKRA
                     LLDAIARATQSIEVERDEIRCRLRAEFDAIVAGQRLSCVFQPIVSLKDFAAIGFELLS
                     RGPVESELHRPDALFEVARNEGRVTELDRLCQRTASKAGEALPPHCLRFVNTEPLTWF
                     LHSQSAGFVQEFVEATPSSHRGLTVIEITENSVIDDFDQMREIVRQLRAHGFRVAIDD
                     AGAGYAGLQTMVEIEPDFIKLDISLIRGVESSIVKQRLVRTLRDFCREAAITLIAEGI
                     ETVKQLDALRELGVSHGQGFLFGLPSADQPVRDAYPPLEAVTPK"
     gene            3910..5274
                     /locus_tag="VFZ86_11220"
     CDS             3910..5274
                     /locus_tag="VFZ86_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HEX5642891.1"
                     /translation="MSEYKKKFVLDEERIPKAWYNIVPDFPEPPAPPLNPATLQPAGL
                     ADLEPIFPMSIIKQEVSAERYIEIPEEVHQIYRMWRPSPLIRATGLEKALDTPAKIFF
                     KYEGVSPAGSHKSNTAVAQAYYNKLDGTTRLATETGAGQWGCALSMACQMFDLECMVY
                     MVKVSFHQKPYRRSMMELWGANVIPSPSDTTNSGRAVLAEDAESLGSLGIAISEAVED
                     AVTHPGSKYALGSVVNHVLLHQTVIGEEAILQCQDADAYPDVVIGCVGGGSSFGGIGF
                     PFLRENLAGKTKTRLIGVEPTACPTLTKGQFVYDFGDEAQSTPLFKMYTLGHDFMPAG
                     IHAGGLRYHAMAPLISHAYHLGLMEAVAVPQTRVFAAAQLFTRTEGILPAPESAHAIR
                     VAIDEALKAKEEGTSKNILFNLTGHGFLDLGAYDAYLSGKLEDYDHPEEAIAASIAKL
                     PKVD"
     gene            complement(5504..6259)
                     /locus_tag="VFZ86_11225"
     CDS             complement(5504..6259)
                     /locus_tag="VFZ86_11225"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEX5642892.1"
                     /translation="MSPSRGRVLLACDLDGTLLDREGVPVAGAGEAVAALISAGALFA
                     VCTGRPLRAARRAAAALGAEPLIYACYHGALVAEASGRVLRHLPLPAREAGEVVGQAL
                     AAGVAVTVWDVDEPRELEPDSGGDAPGDAVSRLVLHGEPGAVARLFGRLRAEWAGRLR
                     VEPIRPGFVGVFAPVVDKGDALRFVAARLGVPGERTVACGDGTADESLLAAAAVRIAV
                     GDPPHVLGHLPDVVVTSWARLPETLRAQVLPLL"
     gene            complement(6280..7755)
                     /locus_tag="VFZ86_11230"
     CDS             complement(6280..7755)
                     /locus_tag="VFZ86_11230"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5642893.1"
                     /translation="MKRRGLRFRLALTHLAIAVLAIVVVGVIVVYTGSRRFDSYLEQV
                     QGKRNAAVLTSLETTYKAPDGWDATAIYALSQVALFNNVDVAVYDTDGRLVFTVQGRH
                     MGRGMMGGQGMMGGGGGMMGGAGGSAPASPQGTPLNAADLTVQSASIVVAGQQVGTAE
                     IYAPKGARAAAEDAYQSALTRNLIIAAAIAGVLALLVSLLVSRRITGPLEELTDAAGD
                     VSAGNLDVRVAPRGDDEVAALASAFNSMADRLARDEQWRRDMTSDLSHELRTPLATIQ
                     SRVEALEDGILPPTPENLRVIGEEVERLGRLLGALRSLNELESEDLSVEHEPVDLEEV
                     AAAAADRHRPAFLTKGVELTEELQPGLVLGDRDRLLQVVANLLDNALKFTPAGGHVVL
                     AVDTVDRRDERAGGALAGPAGRSVRLTVSDDGPGVDAVDLPFVFDRFYRAQETRGTSG
                     AGLGLAICRGLVEAQGGSIAADERPGGGARFTVLLPAAGRA"
     gene            complement(7752..8456)
                     /locus_tag="VFZ86_11235"
     CDS             complement(7752..8456)
                     /locus_tag="VFZ86_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006303759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5642894.1"
                     /translation="MPGPARILVVEDEPRIAEVVQRYLEGQGHSVDRAASGEEALAAA
                     ARRRPDLVILDLGLPGITGEEVCRRLRAESDVPIIMLTAKDAEEDLVRGLQLGADDYV
                     TKPFSPRELTARVRALLRRARAEDEPQADVLERAAGRLLVDSPRRRVTLDGEPVDLTE
                     SEFRLLQALARFPGRVYTRPELVERLQGMEFEPYERTIDAHIKNLRRKLGDDARAPRL
                     VLTVYGRGYAFAEDQR"
     gene            8546..10393
                     /locus_tag="VFZ86_11240"
     CDS             8546..10393
                     /locus_tag="VFZ86_11240"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX5642895.1"
                     /translation="MPATSEEVTLPNLKVYRVAWVVTAVLVVVALFTLGSPDTPRLSQ
                     EPPSFDGEAAYADLKTIVEDFPQRVAGTDPDARMAIWVEEQFRAAGLETHIDPFAATV
                     NGEDVALQNVWGVSAGRRRGTIMVLANRDVPPLATQGAGDNASGVAALLGLADAFSVT
                     AHEHTVIFLCTTGDAFGALGARQFVEAHQTDDLLATVALRDVAKRDSDGVSVDGWSSV
                     PKTAPPWLWLLTAPSARRDSGLEVELPAAPAQMLRLAVPTGAGSQGPFVAAGVPAITV
                     SADGPSAPAPSDTLKTVSVETLTKVGTTAQNMLMAVDAGSEAGAPSGGTIFLTRNATL
                     PGGALAGILAALLLPLLAVAVDLFAHCRRGRIRLRPAFVRAGLHLAPWLVLIAIVYFA
                     NLVGLLPHSPDAVIPPDSRLADTPRYLRVVVLVVLMLLAYFYAVAVERRFERRFATDA
                     RATIFVSHLLLVLIALLLLLVNPYTVLLILPAAILWPLARPGGWARSILPVYLGLLMV
                     PVPLIYYALQLDIGWKVWWYFFLLFENRTIPAAAVLLAALFFSTAGVLAHTLHERGLA
                     PGALSWPAVERRGTDRMGDAEWDAVTEVTTRRTRHGGRARRRSRGPLKR"
     gene            complement(10456..11961)
                     /locus_tag="VFZ86_11245"
     CDS             complement(10456..11961)
                     /locus_tag="VFZ86_11245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEX5642896.1"
                     /translation="MKVMRFLLGLAIGAGVALLVAPKSGRELREQLIGGASGKLLGAA
                     PDEYPQPEPVAEWGAAATAVAEAPPVEESWTPAEPVAEETAVVEPVAEDVVVEPVAEE
                     TAEAVAVEDAVAAEAVAEEAVAQEAPAPKPFPDWEERPGEGAGEDLRARIDETRATLE
                     SELAQPFADETAERADDAAAVADASVVEAVAAEVVAEEAVVEAAAAEIVAEEAVAEAE
                     VAEVVAEEAVLEAVAAEQVAEDAVTGALLADDGDAAAAEFAAEEALAEADVAATVAEQ
                     AVEEADAAELVAAVASEEAAFSEIVAEEAIVEAAVEEAVAEEAVQEAELAEAVAEEAI
                     AEEAVADEVVAEAPLAGVEPEPFAGPAVPPPLAAAWPTEPEPAVTEPVAAEEPPVAEE
                     PEPIVAEEPPVAEEPEPVAAEEPAPSVEETGAQAAAAREGASIDQAEMRRRIEETRAR
                     LKAKAFDAMMSGEAALLSRDSGEKPVPGSDTPELDAETDTAIDESLSQEEY"
     gene            complement(12100..12495)
                     /locus_tag="VFZ86_11250"
     CDS             complement(12100..12495)
                     /locus_tag="VFZ86_11250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642897.1"
                     /translation="MSGVYVGILIFLVVALIALVVVWVLARRMKSDLDTSPSSVAGAR
                     TSLELGPASVTRLRDLSAEPILLKQNEEGVRVQIEHRPMLPLMAFVGKDVSAALNEAA
                     MKVSEQWGPRWVVLLTAGEDGSVTAQRLA"
     gene            complement(12492..13850)
                     /locus_tag="VFZ86_11255"
     CDS             complement(12492..13850)
                     /locus_tag="VFZ86_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019542095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEX5642898.1"
                     /translation="MDDLFSMSDEQVAAEAPLAARMRPRTLAEFSGQEHIIGPGTVLR
                     AAVQGGQLFSMILFGPPGCGKTTLARLIAAETGARLVQLSAVSSGTADVRAAIKGARE
                     ARTLSAARTVLFIDEIHRFNKAQQDALLPAIEDGIVTLIGATTENPYFEVNSALLSRC
                     QLYRFEPLTPELVEEVVTAALQDEERGLGGRGVTLGDGALAFLAGMSRGDARVALNAL
                     ETGWRSRSAASPGAPVELTLDDLQDAAQKSPVMYDREDAHYDTVSAFIKSMRGSDPDA
                     AVYYLASMIAGGEDPKFIARRMIVFASEDVGNADPQALQVAIAVARAVEFVGLPECRI
                     NLSQAATYLALAPKSNAAYTAVDAALDDVRRQGNQPPPPHLRDANYRGAKELGHGAGY
                     KYPHASGGWVSQQYLPDRLSGRRYYVPIRGIESRLAARWEEIRRQAASGEGDGDEEAK
                     RS"
     gene            13968..14471
                     /locus_tag="VFZ86_11260"
     CDS             13968..14471
                     /locus_tag="VFZ86_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEX5642899.1"
                     /translation="MRKVLVLNTTYEPLNVCSVRRALVLLLKDKAEVLEQSGSALHSE
                     RGDYEIPLVIRLTTYVRIPRRMTSARISRRAVFARDKHRCQYCGSARHLTVDHVVPRS
                     RGGSDTWDNLVTSCAPCNRKKADRPLHVAGLRLMRPPRPPGPAAFVLLHVDHVHESWR
                     PYLSYAS"
     gene            complement(14535..16382)
                     /locus_tag="VFZ86_11265"
     CDS             complement(14535..16382)
                     /locus_tag="VFZ86_11265"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5642900.1"
                     /translation="MAVKRTRHGGRLLVTLVVGLLAIGLLWGIAGAMASSESPAADGG
                     KTILRLGWTNDPDNLNPFIGYESSSYEIWAINYELLVGFRASDFANVPGAGLATAWET
                     SADGKVWTFTITDKSKWQDGEPLTASDVAFTFNYCIDNELGMFIDYVKFIDKVEAPDD
                     THVVFTCSKPKANMLGLWIPILPEHIWSKIDPAEVETKFQNPPPIIGSGPFQTVEWKK
                     GEYVRMVANKEYWRGAPKVDEVIYQTYQNSDTMAQDLKTGAIQSGWNIPSAQFDPLNN
                     EPDLESIRAVTIGFDQLGFNCADQKKFPKSTGHPVLQDPAFRTALQWAVDTDKIVSIG
                     YSGNAAAADTLLTRDFYPPDADFHLTPEDPYTFDLEKAKAALDAAGYTDGDGNGIREY
                     EGEDITLRLYARTESTESQNCGKLITGWFEEVGLDIDYEVIDDGALGDKQYAYDGDEF
                     APDFDLFIWGWGGDVDPNFILSIMTTDSIESWSDCNWSNAEYDQLFLEQQTTIDVQER
                     IAIVHRMQQIVYDESPYILLVYPKDLLAANKGTWTGWVRANNDKGSWWYNTQPDTYLA
                     VHPGSAEAAETDGGSSTGLIVGVVVAAAAVILIVVLLLRRRAGRVETEA"
     gene            16483..16758
                     /locus_tag="VFZ86_11270"
     CDS             16483..16758
                     /locus_tag="VFZ86_11270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642901.1"
                     /translation="MIIDDAREPRLQLGDAEPFRIDSAEVTRDIERSTLTSIVLDGEP
                     FPLPVGARVTLWTGPNVVFVGKAVDEHHVLDLLSTETDDELADDEII"
     gene            16783..17847
                     /locus_tag="VFZ86_11275"
     CDS             16783..17847
                     /locus_tag="VFZ86_11275"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HEX5642902.1"
                     /translation="MKGLILSGGAGTRLRPITHTAAKQLVPVANKPVLFYGLEAMKAA
                     GIREVGIVVGDTRAEIEAAVGDGSRFGLAVTYLPQEAPLGLAHAVLISEAFLGDEPFV
                     MYLGDNLLKEGIAPFVQAFAQSDPDALILLQKVADPQSYGIAELDGDGRVARLVEKPA
                     EPRSDLALVGVYLFTQAIFDSVKAITPSARGELEITDAIQHMVDRGLRVEPHVVTGWW
                     KDTGRLEDMLEANRLVLGTIARDVRGELLETTLEGAVQIGAGSRLERCTVRGPVVIGA
                     GCRLTDTFVGPYTSISDGVVVEHAELEHSIVLENSRISHLGTRMSDSLIGRDCVIAHS
                     DARPVAYRFLVGDCCQIGIL"
     gene            17883..18371
                     /locus_tag="VFZ86_11280"
     CDS             17883..18371
                     /locus_tag="VFZ86_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HEX5642903.1"
                     /translation="MIDGVMVKQLKVIPDERGRLVECLRCDDDLFIRFGQFYMTTTFP
                     GVVKGWHLHDAQWDNIVCVKGMIKLVLFDGRGAAGDAAAPGHEPSPTVGQVDEFFLGE
                     HRPLLVRVPPGLWHGWKCVGVDEAYIVNAPTEVYRYDDPDQRELPHDSDAIPYDWGVT
                     LR"
     gene            18391..19236
                     /gene="rfbD"
                     /locus_tag="VFZ86_11285"
     CDS             18391..19236
                     /gene="rfbD"
                     /locus_tag="VFZ86_11285"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEX5642904.1"
                     /translation="MPRWFVTGAGGQLATAFARVLEGEVFLSHESTLDIRDAEAVRTA
                     VHAFAPDAVLHCAAYTDVDGAEADPETAKAVNVLGTRNVVQAVRGTHTAVVYFSTDYV
                     FDGRKSTPYVESDRTRPLNVYGRTKLAGESEVLSWVHGIVFRTAWLFGETGRNFVKTI
                     LAAARERAGTGEPLRVVDDQVGSPTYAGHLAAAVDEALQRGISPGLYHLAGSGYCSWC
                     ELAGEVVDLAGLDVEVEPITTREAGRPARRPAFSALDTERPIPRLPHWADGVATAVDN
                     LIPLG"
     gene            19258..20259
                     /gene="rfbB"
                     /locus_tag="VFZ86_11290"
     CDS             19258..20259
                     /gene="rfbB"
                     /locus_tag="VFZ86_11290"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEX5642905.1"
                     /translation="MRLVVTGGAGFIGANFVRYMLARHDDVEIVNLDKLTYAGNLENL
                     RDVEDDPRYRFVRGDICDAAVVGETLRGADAVVNFAAETHVDRSISGPQDFIRTDVLG
                     THTLLEAVRRLEIPRYLQISTDEVYGSIETGAFTEESDLDPSSPYSASKAGADLLVLA
                     YHRTFGTPVLITRSSNNYGARQYPEKIVPLFITNAIDDLQLPVYGDGLNVRDWLYVDD
                     NCAAIDLVLREGAPGQVYNIGGGNEVQNLTITHRILELLGKGEELIRFVADRPGHDRR
                     YAIDCSKLKALGWRPEVPFEAGIARTVDWYRDNPEWWRPIKSGEWRAYYARQYGEIA"
     gene            20281..20763
                     /locus_tag="VFZ86_11295"
     CDS             20281..20763
                     /locus_tag="VFZ86_11295"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5642906.1"
                     /translation="MATGIPDGYRLERRPPTVRELRALNDAVGWTDLPVDDDAVARGL
                     AASLFGVVVLTAAGETVACARLIGDGGIYYYIQDLIVHPDHQGRGIGDLLMGEVWRYL
                     GEHAGRGAFIGLMAAEGRAGFYERWSFAKRPPGSPGMALGWIPAGPPPTPGSVAPASE
                     "
     gene            complement(20810..21844)
                     /locus_tag="VFZ86_11300"
     CDS             complement(20810..21844)
                     /locus_tag="VFZ86_11300"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEX5642907.1"
                     /translation="MTLTVRPHLAVLMTVLLAVCAPALLLAGPAAAAEPVTLTATISS
                     PDVVYPGSATISGTAPVPGAALSLLELPAGAADWAPAGSATAGGDGAFRFTVAPSVST
                     EYRVVYEPAGQPAAQADVSLRVRPRVTTAFPASLWLGGSVWLRGSVAPAHPGGTVIID
                     RRVDGVWEPFLTATLNADSRFALPWTPGGFGYYRLRARMDADAGHESGAAPSELVIVN
                     RPNAHRVPLRYAHYIVIVRHEYRLYYYEHGALVRAFNVALGRPGYRTPLGYYRVYGKR
                     KPAGGALGSCAMFYRRAGGIAIHGTNEPWLLSRPAPRDFSHGCARMYNSQALWLYART
                     PRGTHVHNLR"
     gene            complement(21856..23199)
                     /locus_tag="VFZ86_11305"
     CDS             complement(21856..23199)
                     /locus_tag="VFZ86_11305"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HEX5642908.1"
                     /translation="MSSRRILRHILTVTTLSAATLLALASPVMAKSYTDVPQSHWAYA
                     SISSVTNRTVAGHRLLDDYKTLFRPERAITRELLARSIVLASGHYGESITPLAIADVP
                     KGYRYYSVIQMAVHLGYMGVDKGGNFRPTEKVAAAAAETVMVRWLKDRYSSYDWSLLG
                     TLAPSRWQPNAGWKTSAPSYLPAIVASRQLMLRYNHPLSADAHETIPGEPIDRAEVAY
                     MFHRAYKVASEWQLSGLGGFKTISFPPLSDRQKQISRFSLKYIGYPYVWAGEYPAKDS
                     PYGAQASGGFDCSGFVFYVMRMHFGYTGITLNQRGASDMAAAAKPRITRSQLKSGDLI
                     FFGPKGPSSTVGSIYHAALYLGRGWFIHSTGSSDGVTLASLNNSSYWKAAFAWGRRVL
                     KPAELVVPNPSPSPTSSQAPVASGSAAPTASPSASVAPASASPMPATASPTPALP"
     gene            23501..25018
                     /locus_tag="VFZ86_11310"
     CDS             23501..25018
                     /locus_tag="VFZ86_11310"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HEX5642909.1"
                     /translation="MPHEPMHTPHPTSPARPATCASPRRGGRRGKLALAGAFVLLILL
                     AVAGPAAAFTSQYAFTISGHGWGHGIGMSQWGAYGYAKHGWTYKAILKHYYTGISFAS
                     VEDSIVRVNLRSGLGAVKLTCPSEYTVQGTGAAWTIPAGTTATTTWTSTGYKVVAGAF
                     NRVFTGDPTFTPSKGALRLITKTDLGDDGAYRGTIRVTHTSGGLMMLNRVRLESYLRG
                     VVPHEVSWTWPREALRAQACAARAYALGSLQPGKSWDVYCDVRDQAYAGAGIEQASTN
                     AAVRDTTGVCPSYGGKPILATYFSSSGGRTENIELAWPGASTIPYLKGVPDPYDTYAT
                     LHDWGPLRRTPSQIGRPLGAAGSVRAVYTVKRGTSPRIVKAAVIGSGGTKYVDGSSLR
                     MKLGLNSTWAVFTSMGISPAARDGAAVGAGGSITLKGRLYPAIADGAKVSLRAYYGGA
                     WHSKGVVTKRVSESLPGGYAARYSAYSVRVAPAATTKYYFSSGKAKSPVTTIKVD"
     gene            complement(25134..>25331)
                     /locus_tag="VFZ86_11315"
     CDS             complement(25134..>25331)
                     /locus_tag="VFZ86_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020678356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HEX5642910.1"
                     /translation="LEHLDAIVLLFSVARYLERLADHATNIAEDVLYMVEGEIQRHNQ
                     SAVAPGTAEELLELGDQGCGD"
BASE COUNT         3744 a         9208 c         8755 g         3624 t
ORIGIN      
        1 aggcgtcagc agtcgtacac gtccgggcgt cgatggctga gtgacggcac ctgcccgcgc
       61 accgcttcga gccgggcgag gtccagatcg gcgacgcaga gaccgacgcc gtcgtcgagc
      121 cgcgccagca cctcgcccca cgggtcgacg atcatgctgt ggccgaagca gtcgcgcccc
      181 ggcagatgct cgcccacctg gtccggggcg acgacgaagc aaccgttctc gatggctcgg
      241 gcgcgcagca gcacctccca gtgcgccgct cccgtgtacg ccgtgaacgc cgccggtacg
      301 cagaggatgc gcgcgccgcg cagggcgagc gagctgtaca ggccggggaa gcgcaggtcg
      361 tagcagatcg agaggccgag cctcaccgca gccccgtcga cggtgtcgat gtcggcggtg
      421 accacttcct cacccggcac gacctcgtcg gactcctggt actgggcggt cgcggtcgtg
      481 acatcgaaga ggtggatctt gcgatacgcg gcgcgcaggt ctccggtcgg gccgaagaga
      541 gcggagacgt tggcgacgag gccgccgggc gccggctcgt agacggaccc ggcgagcagc
      601 cagacgccgc gccgcgccgc caccgcggcg agggcacggt tggacgggcc gtcgggcgcc
      661 tccgcggtgg cgacgatccc ctctcgcggg cccttgaagg cccacgtctc cggcaaggcg
      721 acgaggcgcg cgccccggtc ggccgccgcc tcgaccagat cgacggcggc gcggacgttg
      781 gcggcgacgt cgtcgcgcgt gttcagctgc acgcaggcga cgcgcaggac gcgggccggc
      841 ggcgcgccgg cggacgcggc ctccggcccg gcgctcactc gcaggtcccg ccggaacagg
      901 ccatcagcga cagcagttcg taggcgatgg tcgccgccac cgtcgccgtc aggtggctgc
      961 tgtcgagctc cgggacgacc tcgacgacgt cggcgccggc gaggtcgaga ccgcggcagc
     1021 cgcggatgag cgcgagcgcc tgcgccgagg tcggcccgcc gacctcgggc gtaccgacgg
     1081 ccggcgcaaa gcccgggtcg acgaagtcga cgtcgacggt gatgaacgcc ttggcgtcgc
     1141 cgatggcctc gaccgccgcc gcgaccaccc cggtgcccag ctgcgcgagg tcgtcccacg
     1201 ggaccacggt gaagccgagc tcccgcgcct gctcgtactc gtcgggcgag tcgagcccgc
     1261 cgcgcatccc gaggagagcc gagcgcgacg cgtcgatcac gccctcctcc tgggcgcggc
     1321 ggatcaccgt gctgtggatg tgccgctcgc cggcgccctc gtcgaggcag tccgtgtgcg
     1381 cgccgaagtg cagcagcgcg accgggccgt gcttcgcggc ggcggcgcgc agctcggcca
     1441 gggtgaccgt atggtcgccg ccgacgccca gcggcaccgc gccggcgccg tgcacctggc
     1501 ccagcgtctc ctccatggcg gccaggctgc ggtcgatgaa gccgccggcg accgcccgca
     1561 cgtcgcccgc gtcgaccacg gagagtcgct cgaacaccgc cacacgctgt gcggggttgt
     1621 acgccgggtg gatggtcaag gacgcctcgc gcaccgcacg ggggccgaag cgggctcccg
     1681 tgcgcacggc gaggcccgta tcgaacggga ggccgaccac ggccacatcc acgtcgtcga
     1741 actgcgtgac gtggggcagc cgcaggaacg tggtgacgcc ggcgatccgc ggcgagcgcg
     1801 cgccgccgtc agggccgtat cccttcatca tcgcctcccg cgcctcgagt gccgtccccg
     1861 caagtctact gtccgccgcc gccccgcggc agtgctgctc atccttttcc ccactctgcc
     1921 gataggactc gtgcgcgggc gagcgaacac agtggcacgc ccgcagaccg gcaggaggca
     1981 cggtgccgga cgagaggctc cgcatactgg tgatcgacga cgacggcgac ctgaccgcga
     2041 cggtcgccgc cgtcctcacc gccaacggct acgaggtcct caccgcgacc accggcgagc
     2101 aaggcctgga gctcgccttc accggcaacc cgcacctgat cctcctcgac gtgaccatgc
     2161 cgggcatgga cggccaccag gtctgccgcg agctccagtt cggctacacc aaggacatcc
     2221 ccgtggtgtt cttcaccgcg cgcacggagc tggcgcacat gatggaggcc aaccgctccg
     2281 gcgcgtccgc ctacatcacc aagcccttcc gccccgacca cctgctcgcc acgatcacca
     2341 acgtcctgcg cgactccagc gtctaccacg acgacgtcac cggactgccg acgctggcca
     2401 acgtccagct cgaggtccag cggatgctgt tcgaccacgg gcagctcggc atcatctacg
     2461 tgagcatcgg cggggtgcag gcgctggagc aactgcaggg cttcgaggtc gtcgacctgg
     2521 tgtacagggt cgtcggccag ggcctggaga acgcccgcgg cagcctgatc cgcggcgacg
     2581 acttcgtctc catctccagc ctcggcgacg cgttcctcat cgtcctctcg cccgcccgca
     2641 agaacggctt catcagcgag gaggatctgc tcctggtcaa ggagcgtctc gagaaggagc
     2701 tgctggaccg gctggagaac gacctcgaga gccgcctgct gaccagcgtc gatctcttcg
     2761 tcggctgctc gcggctcacg cagtcgccca agatccgctt caagcgcgcg ctgctcgacg
     2821 ccatcgcgcg cgcgacccag tcgatcgagg tcgagcgcga cgagatccgc tgccgccttc
     2881 gcgcggagtt cgacgcgatc gtcgcagggc agcggctgag ctgcgtgttc cagccgatcg
     2941 tcagcctcaa ggacttcgcg gcgatcggct tcgagctgct gtcgcgcggc cccgtcgaga
     3001 gcgagctcca ccggccggac gcgctcttcg aggtcgcgcg caacgaaggc cgcgtcacgg
     3061 agctcgaccg cctgtgccag cgcacggcct ccaaagccgg cgaagctctc ccgccgcact
     3121 gcctgcgctt cgtgaacacg gagccgctga cgtggttcct gcactcgcag agcgccggct
     3181 tcgtgcagga gttcgtcgag gcgacgcctt cttcgcaccg cggcctcacg gtcatcgaga
     3241 tcaccgagaa cagcgtgatc gacgacttcg accagatgcg cgagatcgtg cgtcagctgc
     3301 gcgcgcacgg cttccgcgtc gccatcgacg acgccggcgc cggctacgcc ggtctccaga
     3361 ccatggtcga gatcgagccc gacttcatca agctcgacat ctcgctgatc cgcggcgtcg
     3421 agtcctccat cgtcaagcag cgtctggtgc gcacgctgcg cgacttctgc cgcgaggccg
     3481 cgatcaccct catcgccgag ggcatcgaga cggtcaagca gctcgatgcc ctgcgagagc
     3541 tcggcgtctc gcacgggcag ggcttcctct tcggcctgcc cagcgcggac cagcccgtgc
     3601 gcgacgcgta cccgccgctg gaggccgtga cccccaagtg agatgctggc ggccgccaga
     3661 cccgtgtgat acagtgccgc gcacgatgac gacccacgac gccaaccgcg gcagcacctc
     3721 ctgcgccaat cggcgtcgct gggcctggcg cgcgtagcgc gcccacttct gacacccgcg
     3781 cgggccctgc ccgcaccccc tgaccgcatc gaagtggtcg tcccgtcccc tcacacggag
     3841 cacaggatca gtgcatcttc atccaggacc gcaacccaag atcgagcgtg attcgagagg
     3901 aggtaggtca tgagcgagta caagaagaag ttcgtccttg acgaggagag gatccccaag
     3961 gcctggtaca acatcgtgcc ggacttcccc gagcccccgg ccccgccgct caacccggcg
     4021 acgctgcagc ccgccggcct cgccgacctc gagcccatct tcccgatgag catcatcaag
     4081 caggaggtct cggccgagcg ctacatcgag atccccgaag aggtccacca gatctatcgc
     4141 atgtggagac cgtcgccgct catccgcgcc accgggctcg agaaggcgct cgacacgccg
     4201 gccaagatct tcttcaagta cgagggcgtc agcccggccg ggtcgcacaa gagcaacacc
     4261 gccgtcgcgc aggcctacta caacaagctg gacggcacca cgcggctcgc caccgagacc
     4321 ggcgccggcc agtggggctg cgccctgagc atggcctgcc agatgttcga cctcgagtgc
     4381 atggtctaca tggtcaaggt gagcttccac cagaagccgt accggcgcag catgatggag
     4441 ctctggggcg ccaacgtgat ccccagtccc agcgacacga cgaactccgg tcgcgccgtg
     4501 ctggccgagg acgccgagtc gctcggtagc ctcggcatcg ccatcagcga ggcagtagag
     4561 gacgccgtca ctcaccccgg ctccaaatac gccctcggca gcgtcgtcaa ccacgtgctc
     4621 ctgcaccaga cggtcatcgg cgaggaggcg atcctccagt gccaggacgc cgacgcctat
     4681 ccggacgtcg tcatcggctg cgtcggcggc ggctcgagct tcggggggat cggcttcccg
     4741 ttcctgcgcg agaacctcgc cggcaagacg aagacgcggc tcatcggcgt ggagcccacc
     4801 gcctgcccga cgctcaccaa gggccagttc gtgtacgact tcggcgacga ggcccagagc
     4861 acgccgctct tcaagatgta cacgctggga cacgacttca tgccggccgg catccacgcc
     4921 ggcggcctgc gctaccacgc gatggcgccg ctcatcagcc acgcctatca tctcggcctg
     4981 atggaggccg tcgccgtgcc gcagaccagg gtgttcgcgg cggcgcagct cttcaccagg
     5041 accgagggta tcctgccggc tcccgagagc gcccacgcga tccgcgtcgc gatcgacgag
     5101 gccctgaagg ccaaggagga gggaacgagc aagaacatcc tcttcaacct caccggtcac
     5161 ggctttctcg acctcggcgc ctacgatgcc tacctcagcg gcaagctcga ggactacgac
     5221 catccggaag aggcgatcgc cgcgtcgatc gcgaagctcc cgaaagtcga ctgagcacag
     5281 cgcggcgggg gatcgccccg ccgcccgcct cgtgcccggg tggagcgtca accgggggtg
     5341 gaggtcgccg ctctccccac gcccggcgtg gcgcgacgtc ccccgaccgg gcgctgggcc
     5401 cttgatctcc tcgaggagat caagggccca gcgtgcgtac gcgacccgcc gagcgctgcc
     5461 gggaccgcct cgcgggacgc acggcggcgc agcggagggg ccgtcagagc agcggcagga
     5521 cctgggcgcg cagagtctcg gggagccgcg cccagctcgt caccacgacg tccggcaggt
     5581 gcccgagcac gtgcggcgga tccccgacgg cgatccgcac cgccgcggcc gcgagcagtg
     5641 actcgtccgc cgttccgtcg ccgcaggcga ccgtccgctc ccccggcacg ccgagccgcg
     5701 cggcgacgaa ccgcagggcg tcgcccttgt caacgacagg cgcgaagacc ccgacgaagc
     5761 cgggccggat gggctcgacg cgcagccgac cggcccactc cgcgcgcagt cggccgaaca
     5821 gccgcgcgac ggcgccgggc tccccgtgga ggacgagccg gctgactgcg tcccccggcg
     5881 cgtcaccccc tgagtccggc tcgagctcgc gcggttcgtc cacgtcccag acggtcacgg
     5941 ccacccccgc cgccagcgcc tggccgacga cctcacccgc ctcgcgggcc gggagaggca
     6001 ggtgccgcaa gaccctgccc gacgcctcgg ccacgagggc cccgtggtag caggcataga
     6061 tgaggggttc cgcgccgagc gcggcggcgg cgcgtcgcgc ggcccgcagc gggcgccccg
     6121 tgcagacggc gaagagggcc cccgcggaaa tcagcgcggc gaccgcctcc cccgcgcccg
     6181 cgaccggtac gccttcgcgg tcgagcaggg tcccgtccag gtcgcacgcg agcaggacac
     6241 ggccgcgcga cggactcatc ggcgcagcca cggcgtgtct caggcgcgtc cggcggccgg
     6301 cagcagcacg gtgaaccggg cgccgccccc gggcctctcg tcggcggcga tcgagccgcc
     6361 ctgcgcctcg accagcccac ggcagatggc caggccaagc ccggcgcccg acgtgccccg
     6421 cgtctcctgc gcgcggtaga accggtcgaa gacgaacggc aagtcgaccg catccacgcc
     6481 cggaccgtcg tcgctgaccg taagacgcac ggaccggccg gcaggccccg ccagggcgcc
     6541 cccggcccgc tcgtcccgcc ggtccacggt gtccacggcg aggaccacgt gtcctccggc
     6601 gggcgtgaac ttgagcgcgt tgtcgagcag gttcgccacc acctgcagca gccggtcgcg
     6661 atcgcccagc acgagccccg gctgcaactc ctccgtgagc tcgacgccct tggtgaggaa
     6721 cgccgggcgg tggcggtcgg cggcggcagc ggccacctcc tccaggtcca cgggctcgtg
     6781 ctccacgctg aggtcctcgc tctcgagctc gttgagactg cgcagcgcac cgagcagccg
     6841 gccgaggcgc tcgacctcct caccgatcac gcgcaggttc tccggcgtgg gcgggaggat
     6901 cccgtcctcc agcgcctcca cccgcgactg gatggtcgcc agcggggtgc gtaactcgtg
     6961 ggagaggtcg ctcgtcatgt cccggcgcca ctgctcgtcg cgggccagcc ggtcggccat
     7021 ggagttgaag gccgaggcca gcgccgcgac ctcgtcgtcg ccccggggcg ccacgcgcac
     7081 gtccaggttg ccggccgaga cgtctccggc ggcgtcggtg agctcctcga gcgggccggt
     7141 gatgcgccgg ctcacgagca ggctcacgag cagggcgagc acgccggcga tggccgcggc
     7201 gatgatgagg ttgcgcgtga gggcgctctg ataggcgtcc tcagcggcgg cgcgcgcgcc
     7261 cttgggcgca tagatctcag cggtgcccac ctgctgcccg gcgacgacga tggacgcgct
     7321 ctgcacggtg aggtcggcgg cgttcagcgg cgtcccctgc ggcgacgcgg gcgccgaccc
     7381 gccggcgccg cccatcatcc cgccgccgcc gcccatcatg ccctggccgc ccatcatgcc
     7441 acggcccatg tgccggccct ggacggtgaa gaccagccgg ccgtcggtgt cgtagaccgc
     7501 cacgtccacg ttgttgaaga gcgccacctg gctgagcgcg tagatggcgg tggcgtccca
     7561 gccgtccggc gccttgtagg tggtctcgag cgaggtgagg acggcggcgt tccgcttgcc
     7621 ctgcacctgc tcgaggtaac tgtcgaagcg ccgcgaaccc gtgtacacga cgatcacccc
     7681 gaccacgacg atggcgagca ccgcgatggc gaggtgggtc agggcgagcc ggaagcggag
     7741 accgcgccgc ttcaccgctg gtcctcggca aaggcgtagc cgcggccgta gaccgtgagc
     7801 acgagccgag gcgcacgggc gtcgtcgccc agcttgcgcc gcaggttctt gatgtgggcg
     7861 tcgatggtgc gctcgtacgg ctcgaactcc atgccctgca ggcgctccac cagttccggc
     7921 cgcgtgtaca cgcggccggg gaaccgggcg agcgcctgga gcagacggaa ctcggactcg
     7981 gtgagatcga cgggttcacc gtccagggtg acgcgccggc gtgggctgtc gaccagcagt
     8041 cgcccggcgg cccgttcgag cacgtcggcc tgcggctcgt cctcggcgcg ggcgcgtcgc
     8101 agcagcgccc gcaccctggc agtcagctcg cgcgggctga agggcttcgt cacgtagtcg
     8161 tcggcgccca gctgcagccc ccgcacgagg tcctcctcgg cgtccttggc ggtcagcatg
     8221 atgatgggca cgtcggattc ggcgcgcagg cgccggcaca cctcctcgcc ggtgatgccg
     8281 gggagaccca ggtcgaggat cacgaggtcg gggcggcgcc gcgcggcggc ggcgagagct
     8341 tcttctccgc tcgccgcccg gtccacgctg tggccctggc cctcgaggta acgctgcacg
     8401 acttcggcga tgcgaggttc gtcttcgacg acgaggatgc gggcgggtcc gggcatgagg
     8461 tcatcatacc cgccggcctc gggccaggcc gcctgagcgc ctccccagcc gaggccccgg
     8521 cctcggctcc gggccatctg ataggatgcc agccacgtcc gaggaggtca ccctgcccaa
     8581 cctgaaggtg taccgagtcg cctgggtggt cacggccgtt ctcgtcgtcg tcgccctgtt
     8641 cacgctcggc agtcccgaca cccccaggct cagccaggag ccgccgtcgt tcgacggcga
     8701 agccgcatac gccgatctca agaccatcgt cgaggacttc ccccagcggg tcgccggcac
     8761 cgatccggac gcgcgcatgg ccatctgggt cgaggagcag ttccgggctg ccgggctcga
     8821 gacgcacatc gatcccttcg cggccacggt caacggcgag gacgtggcgc tgcagaacgt
     8881 ctggggcgtc tcagctggcc gcaggcgcgg caccatcatg gtcctcgcca accgggacgt
     8941 cccgccgctc gccacgcaag gcgcggggga caacgcctcg ggcgtcgccg cgctcctcgg
     9001 cctcgccgac gccttcagcg tcaccgccca cgaacacacc gtcatcttcc tgtgcaccac
     9061 cggcgacgcg ttcggcgcgc tgggcgcgcg gcagttcgtc gaggcgcacc agacggacga
     9121 cctcctcgcg acggtcgcgc tgcgcgacgt cgccaagcgg gacagcgacg gcgtcagcgt
     9181 ggacggctgg agctcggtcc ccaagacggc gccgccgtgg ctctggctgc tcacggcccc
     9241 ttcagccagg agggactcgg gcctcgaggt cgagttgccg gcggcgccgg cgcagatgct
     9301 tcgcctggcg gtccccacgg gcgccggcag ccagggtccg ttcgtggcgg ccggcgtgcc
     9361 ggccatcacg gtgtccgccg acggcccgtc ggcgccggcg ccgagcgaca cgctcaagac
     9421 ggtgtccgtg gagacgctga ccaaggtcgg gaccaccgct cagaacatgc tgatggccgt
     9481 cgacgccggc agcgaggcag gagcgccgag cggcggcacg atcttcctga cgcgtaacgc
     9541 gacgctgccg ggcggcgcgc tcgcgggcat cctcgccgcc ctgctcctgc cgctgctcgc
     9601 ggtcgcggtg gacctgttcg cccactgccg ccgcggccgc atccgcctgc ggccggcctt
     9661 cgtgcgcgca ggcctgcacc tcgccccgtg gctggtgctg atcgccatcg tctacttcgc
     9721 caacctggtg ggcctgctgc cgcacagccc cgacgcggtg atccctccgg attcgcgtct
     9781 tgcggacacc cctcgctacc tgcgcgtcgt cgtgctggtc gtgctgatgc tgctggctta
     9841 cttctacgcg gtcgcagtcg agcgccggtt cgagcgacgc ttcgccaccg acgcgcgcgc
     9901 caccatcttc gtctcccatc tcctgctggt gctcatcgca ctcctgctgc tgctcgtgaa
     9961 cccgtacacg gtgctgctga tcctcccggc cgccatcctc tggccgctcg ccagacccgg
    10021 cgggtgggca cggtcgatcc tccccgtcta cctcggcctg ctgatggtgc cggtgccgct
    10081 catctactac gcgctccagc tcgacatcgg ctggaaggta tggtggtact tcttcctgct
    10141 gttcgagaac cgcaccatcc cggccgccgc cgtcctgctg gcggcgctgt tcttctcgac
    10201 cgccggcgtg ctcgcgcaca ccctgcacga gcgcgggctc gccccgggag cgctctcgtg
    10261 gccggcggtg gagcgccgtg gcacggaccg gatgggcgac gcggaatggg acgccgtcac
    10321 cgaggtcacg acgcgccgca cgcggcacgg cggccgcgca cggcggcgaa gccgcgggcc
    10381 cctcaagcgc tgagaggcca acgacgaggc cggggggctc ccctgcgggc gccccccggc
    10441 ctcgtcacgc tcggctcagt attcttcctg ggagagagac tcgtcgatgg ccgtgtcggt
    10501 ctcggcgtcg agttccgggg tgtcgctgcc cgggacgggc ttctcgccgc tgtcgcgcga
    10561 gagcagggcg gcctcgccgc tcatcatggc gtcgaacgcc ttggccttga ggcgggcgcg
    10621 cgtctcctcg atccggcgac gcatctcggc ctgatcgatc gaggctccct cccgagccgc
    10681 ggcggcctgg gctcccgtct cttcgacgga gggcgccggc tcctcggcgg cgacaggctc
    10741 cggctcctcg gcgactgggg gctcctcggc gacgataggc tccggctcct cggcgactgg
    10801 gggctcctca gcggcgacgg gctcggtaac ggcaggctcg ggctccgtcg gccatgcggc
    10861 cgccagcggg ggcggtacgg caggaccggc gaagggctcg ggctctacgc cggcaagcgg
    10921 cgcctcagcg acgacctcgt ccgcgacggc ttcctcggca atggcttcct ccgcgaccgc
    10981 ctcggcgagt tccgcctcct gcacagcttc ctcggcgacg gcctcttcga cggcggcctc
    11041 cacgatcgcc tcctcggcca cgatctcgga gaacgccgcc tcctcggatg cgacggcggc
    11101 gacgagctcg gccgcgtccg cctcctcgac cgcctgctcc gcgacggtgg cggcgacgtc
    11161 ggcctccgca agcgcctcct cagcagcgaa ctccgcagcg gctgcgtcgc cgtcgtcggc
    11221 gagcagggct ccggtcacgg cgtcttcggc cacctgctcg gccgccacgg cctccaggac
    11281 ggcttcctcg gccacgacct cggcgacttc cgcctcggcg accgcctctt cggcgacgat
    11341 ctccgccgcc gcggcttcca cgacggcctc ctcggccacg acctcggcag ccacggcttc
    11401 cacgactgag gcgtcggcga ctgcggccgc atcgtcggca cgctcggccg tctcgtcggc
    11461 gaacggctgc gccagctcgc tctcgagcgt cgcacgcgtt tcgtcgatgc gtgcgcgcag
    11521 gtcctcgccg gcgccctcgc cggggcgctc ttcccagtcc gggaacggct tgggcgccgg
    11581 ggcctcttgg gcgacggcct cttccgccac ggcctctgcc gccacggcat cctcgaccgc
    11641 gacggcctcg gcagtctcct cggcgacggg ctcgaccacg acatcttccg cgaccggctc
    11701 gacgacggcg gtctcctcgg cgaccggctc ggccggggtc cacgactctt caacgggagg
    11761 cgcttccgcc acggcggtgg ccgcggcgcc ccactcggcg accggctccg gctgcgggta
    11821 ctcgtcggga gcggcgccca gcagcttgcc gctcgcgccg ccgatcagct gctcgcgcag
    11881 ttcacgcccg ctcttcgggg cgacgagcag agcgacgccc gccccgatcg cgagtccgag
    11941 caggaacctc atcaccttca tcgctgcctc cttgaccgct gccgtgccag ccgcacaggc
    12001 gcgcgaagcc ggcgaaagtg tacgtcggaa gaaacgtgcc caggctttac agtttacagc
    12061 cctgtatacg ctgggccccg gtgcagtcct gccggaagat caggccagcc gctgcgccgt
    12121 gacgctgccg tcctctcccg ccgtcaagag gacgacccac cgcggacccc actgctcact
    12181 caccttcatc gccgcctcgt tgagcgcagc gctgacgtcc ttgcccacga aggccatgag
    12241 cggcagcatg ggccggtgct cgatctgcac ccgcacgccc tcctcgttct gcttgagcag
    12301 gatgggctcc gcgctgaggt cgcgaagccg ggtgacgctg gccgggccca gttccaggga
    12361 tgtcctggcg ccggcgaccg agctcggcga tgtgtcgagg tcgctcttca tccggcgggc
    12421 cagcacccag acgacgacca gcgctatgag cgcgacgacg aggaagatga ggatccctac
    12481 atacaccccg ctcacgaccg cttcgcctcc tcgtcaccgt cgccttcgcc ggacgcggcc
    12541 tgccgccgga tctcctccca gcgagccgcc agccggctct cgatccctcg tatcggcacg
    12601 taatagcggc gccctgagag ccggtcgggc aggtactgct ggctgaccca gccgccgctc
    12661 gcgtgcgggt acttgtaccc cgcgccgtgg ccgagttcct tcgcgccccg gtagttggcg
    12721 tcgcggagat gaggcggcgg cggctggttg ccctggcggc gcacgtcgtc gagggccgcg
    12781 tccaccgccg tgtaggcggc gttgctcttg ggcgccagcg ccaggtaggt cgccgcctgg
    12841 ctcaggttga tgcgacactc gggcaagccc acgaactcca cggcgcgcgc aacggcgatc
    12901 gccacctgca gcgcctgcgg gtcggcgttg ccgacgtcct cgctggcgaa gacgatcatg
    12961 cggcgcgcga tgaacttggg gtcctcgccg ccggcgatca tggaggcgag atagtagacc
    13021 gcggcatccg ggtcgctgcc gcgcatgctc ttgatgaacg cggagacggt gtcgtagtgc
    13081 gcatcctcgc ggtcgtacat gacgggggac ttctgcgccg catcctgcag atcgtcgagg
    13141 gtgagctcga cgggcgctcc cggcgacgcc gcggagcggg agcgccagcc cgtctcgagg
    13201 gcgttgagcg cgacgcgcgc gtcgccgcgc gacatgccgg cgaggaaggc gagcgcgccg
    13261 tcgccgagcg tgacgcccct gccgcccagg ccgcgctcct cgtcctgcag cgcggccgtc
    13321 acgacctcct cgaccagttc gggtgtgagc ggctcgaagc ggtagagctg gcagcggctg
    13381 agcagcgccg agttgacctc gaagtagggg ttctcggtgg tcgcgccgat gagggtgacg
    13441 atcccatcct ctatcgccgg caggagcgcg tcctgctgcg ccttgttgaa gcggtggatc
    13501 tcatcgatga agagcacggt gcgcgccgcg ctgagggtgc gcgcctcccg cgcgcccttg
    13561 atcgcggcgc gcacgtccgc ggtgccggag ctcaccgccg agagctgcac cagccgggcg
    13621 ccggtctcgg cggcgatgag ccgtgccagc gtggtcttgc cgcagcccgg cggcccgaag
    13681 aggatcatgc tgaacagctg gccgccctgg acggcggcgc ggagcaccgt gcccggcccg
    13741 atgatgtgct cctgcccgga gaactccgcg agagtacgcg gccgcatgcg cgccgccagc
    13801 ggcgcctcgg ccgcgacctg ctcgtcgctc atggagaaga ggtcgtccat ccagcccagt
    13861 gtatgctcgc cgcaggccaa gcatcgagcg gggcgccaac gagccagggt accggctcgg
    13921 atgtcgcaga ccaggagcac cgctcaatct gctactattc cgggacgatg cgcaaggtgc
    13981 tcgtcctgaa caccacctac gagccactca acgtgtgctc cgtacgacgt gcgctcgttc
    14041 tcctgctcaa ggacaaagcc gaagtactcg agcaatcggg gtcggccctg cacagcgagc
    14101 gcggcgacta cgagatcccc ctcgtcatcc gcctcacgac ctacgtccgc atcccgcgcc
    14161 ggatgacgtc cgcgcgcatc tcccgccgcg cggtcttcgc ccgcgacaag caccgctgcc
    14221 agtactgcgg cagcgcgcgg cacctcaccg tggaccacgt ggtgccgcgg tcccggggcg
    14281 gctcggacac ctgggacaac ctcgtcacgt cgtgcgcgcc ctgcaaccgg aagaaggcgg
    14341 acaggccgct gcacgtggcg gggctgcggc tgatgcggcc gccgcggcct ccggggccgg
    14401 cggcgttcgt gctgctccac gtggaccatg tccacgagag ctggcggccc tacctcagct
    14461 acgcctcgta gactgcaccg gaagggtgcg ggaggggggc gccggggctc gcgtcccggc
    14521 gcccccctcg ccgctcaggc ctcggtctcg acgcgccctg cccgtctccg cagcagcagg
    14581 acgacgatga ggatgacggc ggcggcggcg accacgaccc ccacgatgag gcccgtgctg
    14641 gacccgccgt cagtctcggc ggcctcggcg ctgccggggt gcaccgcgag gtaggtgtcc
    14701 ggctgcgtgt tgtaccacca ggagcccttg tcgttgttgg cgcgcaccca tccggtccac
    14761 gtgcccttgt tggccgcgag gaggtccttg gggtacacga gcaggatgta cggcgattcg
    14821 tcgtagacga tctgctgcat cctgtggacg atcgcgatgc gctcctgcac gtcgatcgtc
    14881 gtctgctgct cgaggaacaa ctggtcgtac tccgcgttgg accagttgca gtcgctccag
    14941 ctctcgatcg agtccgtggt catgatcgag aggatgaagt tcgggtcgac gtcgccgccc
    15001 cagccccaga tgaagaggtc gaagtcgggg gcgaactcgt cgccgtcgta ggcgtactgc
    15061 ttgtcaccga gggcgccgtc gtcgatgacc tcgtagtcga tgtcgaggcc gacctcctcg
    15121 aaccagccgg tgatgagctt gccgcagttc tggctctccg tcgactccgt gcgcgcgtag
    15181 aggcgcagcg tgatgtcctc gccctcgtac tcgcggatgc cgttgccgtc cccgtcggtg
    15241 tacccggccg cgtcgagagc agccttggcc ttctcgaggt cgaacgtgta cgggtcctcg
    15301 ggcgtgaggt ggaagtccgc gtcgggcgga tagaagtccc tggtgagaag cgtgtcggcc
    15361 gcggcggcgt tgccgctgta gccgatggag acgatcttgt ccgtgtcgac ggcccactgc
    15421 agggcggtgc ggaacgccgg gtcctgcagc acgggatgac ccgtcgactt ggggaacttc
    15481 ttctggtcgg cgcagttgaa gccgagctgg tcgaagccga tcgtgaccgc gcggatggat
    15541 tcgaggtcgg gctcgttgtt cagcgggtcg aactgcgcgg acgggatgtt ccagccgctc
    15601 tggatggcgc cggtcttgag gtcctgcgcc atcgtgtcgg agttctgata ggtctggtag
    15661 atgacctcgt cgactttggg ggcgccgcgc cagtactcct tgttggcgac catgcgcacg
    15721 tactcgccct tcttccattc caccgtctgg aaggggccgg agccgatgat cggcggtggg
    15781 ttctggaact tcgtctcgac ctcggcgggg tcgatcttgc tccagatgtg ctcgggcagg
    15841 atggggatcc acaacccgag catgttcgcc ttgggcttgg agcaggtgaa caccacgtgg
    15901 gtgtcgtccg gcgcttcgac cttgtcgatg aacttgacgt agtcgatgaa catgcccagc
    15961 tcgttgtcga tgcagtagtt gaaggtgaag gcgacgtcgc tcgccgtcag cggctcgccg
    16021 tcctgccact tcgacttgtc ggtgatcgtg aacgtccaga ccttgccgtc ggcggacgtc
    16081 tcccaggcag tcgccaggcc cgccccgggg acgttggcga aatcgctggc gcggaatccg
    16141 accagcagct cgtagttgat cgcccagatc tcgtagctgg acgactcgta gccgatgaac
    16201 gggttgaggt tgtccggatc gttggtccat ccgagccgca ggatggtctt gccgccgtcg
    16261 gcggcggggc tctcgctcga ggccatcgcg ccggcgattc cccagagaag cccgatggcc
    16321 agcagaccga ccaccagcgt gacgagcagc cggcccccgt gtcgtgtgcg tttgacggcc
    16381 acgttcgctc ctttcattcc cccccgcaga accgtgcagg tccctctact caagcacgaa
    16441 tccgggactt cagccagtct cgcgggtggt atactccgcg ccgtgatcat cgacgacgcc
    16501 agagagccac ggctgcagct gggcgacgcg gagccgttcc gcatcgacag cgccgaggtc
    16561 acgcgcgaca tcgagcgctc cacgctgacc agcatcgtcc tcgacggcga gccgttcccg
    16621 ctgccggtcg gggcccgcgt cactctgtgg acgggcccca atgtcgtctt cgtcggtaag
    16681 gccgtagacg aacatcacgt cctcgacctc ctctccaccg agacagacga cgagctcgcc
    16741 gacgacgaga tcatctgact cgcgggcgcg gggggcacgc ccatgaaagg cctcatcctc
    16801 agcggcggcg ccgggacgcg cctgcggccc atcacccaca ccgccgccaa gcagctcgtg
    16861 ccggtcgcga acaagccggt gctcttctac ggcctcgagg ccatgaaggc cgcggggatc
    16921 cgcgaggtcg gcatcgtcgt cggcgacacg cgggccgaga tcgaggccgc ggtgggcgac
    16981 ggcagccgct tcgggctcgc ggtcacctac ctgccgcagg aggcgccgct gggcctcgcg
    17041 cacgcggtcc tcatcagcga ggcgttcctc ggcgacgagc ccttcgtcat gtacctcggc
    17101 gacaacctgc tcaaagaggg catcgcgccg ttcgtgcagg cgttcgcgca gagcgacccc
    17161 gacgcgctca tcctcctgca gaaggtcgcc gacccgcaga gctacggcat cgccgagctc
    17221 gacggcgacg gccgcgtggc gcggctcgtg gagaagccgg ccgagccgcg cagcgacctc
    17281 gccctggtgg gcgtgtacct gttcacgcag gccatcttcg acagcgtcaa agccatcacg
    17341 cccagcgccc gcggcgagct cgagatcacc gacgccatcc agcacatggt cgaccgcggc
    17401 ctgcgcgtcg agccgcacgt ggtcaccggc tggtggaagg acaccggcag gctcgaggac
    17461 atgctcgagg ccaaccgtct ggtgctcggc accatcgcgc gcgacgtccg cggcgaactg
    17521 ctcgagacca cgctcgaggg agcggtgcag atcggcgccg gcagccggct ggagcgctgt
    17581 accgtgcgcg gcccggtggt catcggcgcc gggtgcaggc tcacggacac cttcgtcggc
    17641 ccgtacacgt ccatcagcga cggcgtggtc gtcgagcacg ccgagctgga gcactcgatc
    17701 gtcctcgaga acagccgcat ctcgcacctg ggcacgcgta tgtccgacag cctcatcggt
    17761 cgcgactgcg tcatcgccca cagcgacgcc cgcccggtgg cctaccgctt cctggtcggc
    17821 gactgctgcc agatcggcat cctgtagacg caccccgggc gagcacgggg aggagggacc
    17881 caatgatcga cggcgtcatg gtcaagcagc tcaaggtgat ccccgacgag cgcggccgcc
    17941 tcgtcgaatg cctgcgctgc gacgacgacc tcttcatccg cttcggccag ttctacatga
    18001 cgaccacgtt ccccggcgtc gtcaaggggt ggcacctcca cgacgcccag tgggacaaca
    18061 tcgtgtgcgt caagggcatg atcaagctcg tgctgttcga cggccgcggg gccgcaggcg
    18121 acgcggccgc gccggggcac gagccctcgc cgacggtcgg ccaggtcgac gagttcttcc
    18181 tcggcgagca ccggccgctg ctggtgcgcg tcccgccggg cctctggcac ggctggaagt
    18241 gcgtcggcgt cgacgaggcc tacatcgtca acgcgccgac cgaggtgtac aggtacgacg
    18301 accccgacca gcgggaactg ccgcacgaca gtgacgcgat cccctacgac tggggcgtca
    18361 cgctgcgcta ggcgcggccg gcgaagctgt atgccgcgct ggttcgtcac aggggccggg
    18421 ggccaactgg ccacggcctt cgcgcgcgtc ctcgagggcg aggtcttcct cagccacgag
    18481 agcacgctgg acatccggga cgccgaggcc gtgcgcacgg ccgtgcacgc gttcgccccc
    18541 gacgccgtgc tgcactgcgc cgcctacacc gacgtggacg gcgccgaggc cgatccggag
    18601 acggcaaagg cggtcaacgt gctcggcacg cgcaatgtcg tccaggcagt ccgcgggacg
    18661 cacaccgccg tggtctactt cagcaccgac tacgtcttcg acgggcggaa gtccaccccg
    18721 tacgtggaga gcgaccgcac gcgcccgctc aacgtgtacg gccgcaccaa gctcgccggc
    18781 gagagcgagg tcctgagctg ggtgcacggc atcgtcttcc ggaccgcctg gctgttcggc
    18841 gagaccggcc gcaacttcgt gaagacgatc ctcgctgcgg cgcgggagcg cgccgggacg
    18901 ggcgagcccc tccgggtcgt ggacgaccag gtcggctcgc ccacctacgc cggtcacctc
    18961 gctgcggccg tcgacgaggc gctgcagcgg ggtatcagcc ccggcctcta ccacctcgcg
    19021 gggagcgggt actgctcctg gtgcgagctg gccggcgagg tcgtcgacct cgccggcctc
    19081 gacgtggagg tcgagccgat caccacccgt gaggcgggcc ggccggcgcg ccggccggcc
    19141 ttctcggcgc tcgacacgga gcgtcccatc ccccgtctcc cccactgggc ggacggcgtg
    19201 gccacggccg tcgacaatct catcccgctc ggataggaag accggaggag gcacaccgtg
    19261 aggctcgtgg tcaccggcgg cgccgggttc atcggcgcca acttcgtgcg ctacatgctc
    19321 gcccggcacg acgacgtcga gatcgtgaac ctcgacaagc tcacctacgc cggcaacctc
    19381 gagaacctgc gcgacgtcga ggacgacccg cggtacaggt tcgtacgcgg cgacatctgc
    19441 gacgcggccg tcgtcggcga gacgctgcgc ggcgccgacg ccgtcgtcaa cttcgccgcc
    19501 gagacccacg tcgaccgctc catcagcggg ccgcaggact tcatccgcac ggacgtgctc
    19561 ggcacccaca ccctcctcga ggcggtgcgc cgactggaga tcccgcgcta cctgcagatc
    19621 agcaccgacg aggtctacgg cagcatcgag accggggcgt tcacggagga gtccgacctc
    19681 gacccctcca gcccgtactc ggcgagcaag gccggcgccg acctcctcgt actcgcctac
    19741 caccgcacct tcggcacgcc ggtgctcatc acgcgcagtt ccaacaacta cggcgcccgg
    19801 cagtacccgg agaagatcgt cccgctgttc atcaccaacg ccatcgacga cctgcaactg
    19861 cctgtgtacg gcgacgggct caacgtccgc gactggctgt acgtggacga caactgcgcg
    19921 gcaatcgacc tcgtgctgcg cgagggcgcg ccggggcagg tctacaacat cggcggcggc
    19981 aacgaggtac agaacctcac gatcacgcac cgcatcctgg agctgctggg caagggcgag
    20041 gagctgatcc gcttcgtcgc cgaccgcccc ggccacgacc gccgctacgc gatcgactgc
    20101 tcgaagctca aggccctggg ctggcgcccg gaggtgccgt tcgaggccgg gatcgcgcgc
    20161 acggtggact ggtaccgcga caacccggaa tggtggcggc cgatcaagtc gggggagtgg
    20221 cgcgcttact acgcccgcca gtacggcgag atcgcctgag cgccgtcggt gggtccccgc
    20281 gtggcgacag gaatcccaga cgggtacaga ctcgagcggc gcccgccgac cgtccgggag
    20341 ctgcgggcgc tcaacgatgc ggtcggctgg accgatcttc ccgtggacga cgacgccgtg
    20401 gcgcgaggcc tcgcggcctc gctgttcggc gtcgtcgtgc tcaccgccgc cggcgagacc
    20461 gtcgcttgcg cccgcctcat cggggacggc ggcatctact actacatcca ggacctcatc
    20521 gtgcatcccg accaccaggg gcgcgggatc ggcgacctcc tcatggggga ggtgtggcgc
    20581 tacctggggg agcacgccgg ccgaggcgcg ttcatcggcc tgatggcggc ggagggcaga
    20641 gcgggcttct acgaacgctg gagcttcgcg aagcggccac cggggagtcc gggcatggcc
    20701 cttggctgga ttccggcagg ccctccgccg acacccggct ccgtggcgcc cgcctcggag
    20761 tgatctgaag ccgccggtct gcgggacggc cacggcgggc cggcgccggt cagcgcaggt
    20821 tgtgcacgtg cgtcccgcgc ggcgtgcgcg cgtagagcca cagcgcctgg ctgttgtaca
    20881 tgcgcgcgca gccgtgggag aagtcccgcg gggcagggcg gctcagcagc cacggctcgt
    20941 tcgtgccgtg gatggcgata ccgcccgcgc gacggtagaa catggcgcag gagccgagcg
    21001 cgccgccggc gggcttgcgc ttgccgtaga cacggtagta gccgagcggg gtgcggtagc
    21061 cggggcgccc gagcgccacg ttgaaggcgc ggacgagggc gccgtgctcg tagtagtaca
    21121 gccggtactc gtggcggacg atcacgatgt agtgcgcgta gcgcagcggc accctgtggg
    21181 cgttcggccg gttcacgatg acgagctccg aaggcgccgc accgctctcg tgcccggcgt
    21241 cggcgtccat gcgcgcgcgc aggcggtagt agccgaagcc gccgggcgtc cacgggagcg
    21301 cgaagcgcga gtcggcgttc agcgtggcgg tcaggaacgg ctcccagacg ccgtcgaccc
    21361 ggcggtcgat gatcacggtc cctccgggat gcgccggcgc gacggagccc cgcagccaga
    21421 ccgagccgcc cagccagaga ctggcaggaa acgcggtcgt cacacgggga cgcacgcgca
    21481 ggctcacgtc cgcctgcgcc gccggctggc cggcgggctc gtacaccacg cggtactcgg
    21541 tggagacgga cggcgcgacg gtgaacctga acgcaccgtc accaccggcc gtcgcggacc
    21601 ccgccggcgc ccaatccgcg gcgccggccg ggagctcgag cagcgagagc gcggcgccgg
    21661 gtacgggagc tgttccgctg atggtcgccg accccgggta gacgacgtcc gggctggaga
    21721 tcgtcgcggt cagcgtcacc ggctccgcgg cggccgccgg accggcgagg aggagcgcag
    21781 gagcgcagac ggccagcagc acggtcatca gcacggccag gtgagggcgg acggtcaggg
    21841 tcacgagccg aacggtcagg gcagcgccgg ggtcggcgac gcggtcgcag gcatgggcga
    21901 ggcggacgcc ggggcgacgg aggccgaggg agacgctgtg ggagccgcgg acccgctcgc
    21961 gacgggcgcc tgcgacgacg tgggcgacgg cgacgggttc ggcacgacga gctccgccgg
    22021 cttgagcacg cggcgacccc aggcgaaggc cgccttccag tagctggagt tgttcagcga
    22081 cgccagggtc acgccgtcgc tggagccggt ggagtggatg aaccagccgc ggccgagata
    22141 cagggcggcg tggtagatgg accccactgt cgagctcggc cccttgggcc cgaagaagat
    22201 caggtcgccc gacttgagct gactgcgggt gatgcgcggc ttggccgcag cagccatgtc
    22261 cgaggcgcca cgctggttca gggtgatgcc cgtgtagccg aagtgcatcc gcatcacgta
    22321 gaagacgaac ccggagcagt cgaaaccgcc ggacgcctgg gcgccgtacg gtgagtcctt
    22381 ggcggggtac tcgcccgccc agacgtatgg gtagccgatg tacttgagcg agaacctgga
    22441 gatctgcttc tgccgatcgc tgaggggcgg gaagctgatc gtcttgaacc cgccaaggcc
    22501 ggagagctgc cactcgcttg ccaccttgta ggcccggtgg aacatgtagg ccacctcggc
    22561 ccgatcgatc ggctcgccgg ggatggtctc gtgcgcatcc gcgctgagcg ggtggttgta
    22621 gcgcagcatc agctgccgcg aggcgacgat ggcggggagg taggagggcg cgctcgtctt
    22681 ccaccccgcg ttcggctgcc agcgcgacgg cgccagcgtg ccgagcagcg accagtcgta
    22741 ggaggagtac ctgtccttga gccagcggac catcacggtc tccgcagcgg cggcggcgac
    22801 cttctccgtg ggccggaagt tgccgccctt gtccacgccc atgtagccca ggtgcacggc
    22861 catctggatc acggagtagt aacggtaccc cttgggaacg tcggcgatgg ccagcggcgt
    22921 gatgctctcg ccgtagtggc ccgaggcgag gacgatcgag cgcgcgagga gctctcgcgt
    22981 gatcgcgcgc tccggccgga acagggtctt gtagtcgtcc agcagccggt ggccggccac
    23041 cgtgcggttg gtcaccgacg agatggaggc gtaggcccaa tgactctgcg gcacgtcggt
    23101 gtacgacttg gccatgaccg gcgaagcgag ggcgagcagg gtcgccgccg agagcgtggt
    23161 gaccgtgagg atgtggcgga ggatgcggcg tgagctcatg tctcgtactg ctatcggcgt
    23221 caaggccggc tgcttgagcg cagcccgagc ctgcctcgcg ggcccggcca cgacttcgcc
    23281 gccgggctcg cctttcccct ctactggtcg ccgagggtag cacgcggcgg gcggcggcgc
    23341 gtccccgtcg cagttcgcgg ccggccgacg tggccgtcgt tgcgagcccc acgcctcaag
    23401 aagaggagac cacgggtcga tgccagaagc agtcacgacc actgcaggcc cgtttgcccc
    23461 gccggagcgg ggaagggtat cctcgagcga ggaggcgctg gtgcctcacg agccaatgca
    23521 caccccgcac cccacatccc cagcccgtcc agcgacgtgc gcgtcgccgc gtcgcggtgg
    23581 gcgccggggc aagctcgcgc tcgccggcgc cttcgtcctc ctcatcctgc tcgccgtcgc
    23641 cggaccggca gccgccttca cgagccagta cgccttcacc atcagcggcc acggctgggg
    23701 acacggcatc ggcatgtcgc aatggggggc ctacggctac gccaagcacg gctggaccta
    23761 caaggccatc ctgaagcact actacaccgg catctcgttc gcgagcgtcg aggactcgat
    23821 cgtccgcgtc aacctgcgca gcgggctcgg cgccgtcaag ctcacctgcc ccagcgagta
    23881 cacggtccag gggaccggcg ctgcgtggac catccccgcc ggcacgaccg cgaccaccac
    23941 ctggacgagc accggctaca aggtcgtcgc cggagccttc aacagggtct tcaccggcga
    24001 cccgaccttc acgccgtcca agggcgcgct gcgcctcatc accaagacgg acctcggcga
    24061 cgacggcgcc taccgcggca cgatcagggt cacccacacg agcggcggcc tcatgatgct
    24121 caaccgcgtg cggctcgaga gctacctgcg cggcgtggtc ccgcacgagg tgtcgtggac
    24181 atggccacgg gaggcgctcc gggcgcaggc ctgcgccgcc cgcgcctacg cgctgggcag
    24241 tctccagccg ggcaagtcat gggacgtcta ctgcgacgtc cgcgaccagg cctacgccgg
    24301 cgcggggatc gagcaagcca gcaccaacgc cgccgtgcgc gacaccacgg gcgtatgccc
    24361 aagctacggc ggcaagccga tcctggccac atacttctcc agctcgggcg gccgcaccga
    24421 gaacatcgaa ctcgcgtggc cgggagcgtc gacgatcccc tatctcaagg gcgttcccga
    24481 cccctacgac acgtacgcca cgctgcacga ctggggaccg ctgcgccgca cgccgtccca
    24541 gatcggccgg ccgctcggcg ccgccgggtc ggttcgcgcc gtctacaccg tgaagcgcgg
    24601 cacatctccg cgcatcgtca aggcggcggt catcggcagc ggcggcacca agtacgtcga
    24661 cggcagctcg ttgcgcatga agctcgggct gaacagcacc tgggcggtgt tcaccagcat
    24721 gggcatcagt cccgccgccc gcgatggagc cgccgtcggc gcgggcggca gcatcacgct
    24781 caagggtcgc ctctacccgg cgatcgccga cggggcgaag gtctccctgc gcgcctacta
    24841 cggcggcgcc tggcacagca agggagtcgt caccaagcgc gtctccgaga gcctgcctgg
    24901 ggggtacgcg gcgcgctact ccgcgtacag cgtcagggtc gccccggccg ccaccaccaa
    24961 gtactacttc tcgagcggca aggccaagtc gcccgtcacg accatcaagg tggactagct
    25021 ccgtacgcgc ggcggcccgg gcatggtagg ggaagcccgg gccgcctgtg ccctccgcgc
    25081 gccggaacag gatcgacggc tgggagggcg agcttgagcg tcacgcaggc tggtcagtca
    25141 ccacatccct gatcgccgag ctccaggagc tcctcggcgg tccccggcgc cactgccgac
    25201 tggttgtgcc gctggatctc gccttcgacc atgtagagga cgtcctcggc gatgttggtg
    25261 gcgtggtccg ccagccgctc gaggtagcgc gcgacgctga acagcagcac gatcgcgtcg
    25321 agatgctcga g
//
LOCUS       DASUMX010000240        31289 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1236324, whole genome shotgun sequence.
ACCESSION   DASUMX010000240 DASUMX010000000
VERSION     DASUMX010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 31289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31289
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1236324"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..1790)
                     /locus_tag="VFZ86_11320"
     CDS             complement(45..1790)
                     /locus_tag="VFZ86_11320"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX5642911.1"
                     /translation="MATDASKTGEQTLFVRKASGLVKGWSSMDGFRYSFFSVNLFLAI
                     WSFAYATFIPGGSLFWAIVITAIFIVLEIIVYAGLISAMPRAGGDYVWITRIFHSSIG
                     FVIAGVGWWFILWLWTPIYADMGVQTTVKPIFRILGLHGAADWLGTATGIFVASLVVI
                     AIASILVAVGMKTYANFQKWAMWIGLAGVIICGILLLVTSGDSFKEKFNQAAEKYYGG
                     DAALLDLTVYEQDEAGLFVYDDAGALVPVEGETHAGAFEAIEAVGAGTGMPTSTFSGG
                     LSWATWTIIPFMLFWMAWPNWGATLYGEVRGAKDFRKNIYQMLGGLLVPTIIALSFLG
                     LMTWKVGYQAFMGTMAAFWWGISPLGGDYISPTAMISWIVPNAAFQVVLVAAISLLLF
                     GWFGTLFLSSTRMIFASAFDRILPERAAKVTSGGVPTVALLLMTIPSIIVAALYAYTP
                     VVEERGVTIVKLFTYDATFGIIIMFLFSGLAFMVMKWRARGIWENSALPKSGIAGIPW
                     MAIIAALYCAFMVFNIFMFVWDPDGVYYINLWESAVFMGVLYLAGVVIWLIAWMVRKK
                     QGMELEAVAKEIPVE"
     gene            complement(1873..2820)
                     /locus_tag="VFZ86_11325"
     CDS             complement(1873..2820)
                     /locus_tag="VFZ86_11325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX5642912.1"
                     /translation="MKVRLFFATDVHGSEVCWRKFINSAKHYEADVLILGGDMTGKAI
                     VPIVQTGPEQWRYHMLDITHDLNGAEDLAKAERLIKDHGYYPVALTPEERDEYTKDED
                     KLEELFHEQMRKTVADWVEWADAKLEGSGIQCIVCPGNDDRWDIDEILATSKHMQVGE
                     GNVLDLPEGYQLASSGWTNLTPWDTPREEDEPKLKERIMGIVKQATVPPERLVLGLHA
                     PPYDTQLDVAPKIDWETLTVQGQDSAHVGSTAVKEVIEEVQPILSLHGHIHESRAAVR
                     IGRTLAINPGSSYVEGMVAGVVVDLDGKSKLKRYRLTSG"
     gene            complement(2864..3799)
                     /locus_tag="VFZ86_11330"
     CDS             complement(2864..3799)
                     /locus_tag="VFZ86_11330"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline kinase family protein"
                     /protein_id="HEX5642913.1"
                     /translation="MATEAHHLSPDELIARIDDWKGKEIRWEELGGGITNHNYIVWVN
                     GGWGNPGGGKYVLRVPGAGTDMFIDRDIERACMLEVARIGTSPRVAHVIEPENAMVID
                     FIDGEVMHPDTMAGHPERIKQAVEAVRVYHDKAVFSNKIAIFDMLRRYTKVAQDINAP
                     MPERLAALLERMPEIEEAVGRNPVPDVACQNDLLSENFIIDADGRMWIIDWEYGGMTD
                     PYFDLGDHVMEHPYSREEERLIIETYLGEMDERLFGRMMLYKIVSAVWWDVWAMIQHT
                     VSKIDFDYMEWAMERVGRAERGIADPDYTKWLAAQ"
     gene            complement(3954..5336)
                     /locus_tag="VFZ86_11335"
     CDS             complement(3954..5336)
                     /locus_tag="VFZ86_11335"
                     /inference="COORDINATES: protein motif:HMM:NF017207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monomethylamine:corrinoid methyltransferase"
                     /protein_id="HEX5642914.1"
                     /translation="MIDLIEVFDRAETGPLTSETDYYMKRYVPKLTEVIARHKVKWDK
                     ETIINADDTLTDAVFEAAVDLIAEAGAYCPETNRVMQFTRDEIVVACEQAPKATTFGE
                     GRERNVMRGRRPDTTDDRPWVHVGGGIYTTDEGVFVDTVEKMASYNIVDSVSVPSILH
                     LRGKDSRVRSPQEILSACKTVRLAREGIRRSGRAGMPIINGISAASQPASMFAASYPD
                     FGLRPSDGFLIDFLSEMVVGYEALQKVAFYNSISANIGSTSTPLFGGYAGGAEGVAVT
                     ATAYMIMGNLVFRGSYHYNAPLHSKLHLSSTKPLIWAIAMSNAAIGRNMQYPMVNLPY
                     LGGGSGTKQYHYEMACYMLAVVTSGGNVFSGHPAMAVQSDSLVPDDHKFHAEIGLAAS
                     KLTRAQAEPIAQKLFPLFGDKLKDPDKGLTFQEVYDMRTKRIVNADYQKAMDEVREEL
                     AGMGLEVPVS"
     gene            complement(5374..6021)
                     /locus_tag="VFZ86_11340"
     CDS             complement(5374..6021)
                     /locus_tag="VFZ86_11340"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HEX5642915.1"
                     /translation="MADAEHFDKLKQSIIDQDEDQVLAAVNAALDDGVEAKAIIDQGL
                     LPGLNVIGEQFECEDIFLPELMQAALAFQSAMVILEPKIKEQGAEGEKKGKIVLGTVK
                     GDLHSIGKNILKLLYETSGFEVWDLGIDVDLFQFVDKAKEVDADIIAMSALLTTTLVG
                     QKDVIEALRDQGARDKFKVIIGGGATTKEWADTIGADGWAATAYEAVTLSTKLVS"
     gene            complement(6095..7897)
                     /locus_tag="VFZ86_11345"
     CDS             complement(6095..7897)
                     /locus_tag="VFZ86_11345"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HEX5642916.1"
                     /translation="MGGQGRRPAAHGQGRDLPQGLSRRGVSLRDARPGHRRRAPGAAA
                     HAGDRRAVRGQLGHRRVLHRPRPERRRIPLLPRAQRGVRRFHRGPPRRAGRHGRTRQG
                     ARPRGRRERGGTTMRYVELLTEAEVRRTHEASLEVLETVGILAHFAPAREVFKKHGCI
                     VDDETGLVRFPAEIVEKYRKLAPPSFTFRGRDPQYDRTIPDDGPIVVTGSSAPNVVDP
                     VTGVERRATSADIANIAHLINELPGFDVFSISTLADDAPEGQFSLSRFYPALKNCLKP
                     VRSNTPNMSDLQQVVELGELIAGGREAYAERPCINHHYCPMVSPLTMDVDSTEAVMWL
                     TERGLPVYTTVVPNAGLTSPMTLMGTLVVGNAEFLAITVFQQMVKEGTPIIYASLPTV
                     ADMRTGAYAPGAIETGILLAAQAQLARFYGVPSGGYIGLTNAHSNDAQAGYETGMNTV
                     LGVSAGATLLNMGGLYSSLMAFDFAKAVVDNEIAIMDKRAYRGLEFSEENLALDEIAA
                     VGPGGSYMGRKHTKARMKTTAVLPRLANRDMRQQWEDAGRPTINDRALAEARRILAQP
                     NEAVWDGELDARIREHFAGLVAGDATPLEADTTS"
     gene            complement(7879..8766)
                     /locus_tag="VFZ86_11350"
                     /pseudo
     CDS             complement(7879..8766)
                     /locus_tag="VFZ86_11350"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
     gene            complement(8814..9605)
                     /locus_tag="VFZ86_11355"
     CDS             complement(8814..9605)
                     /locus_tag="VFZ86_11355"
                     /inference="COORDINATES: protein motif:HMM:NF012669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HEX5642917.1"
                     /translation="MSSEVPEVHEMIPAERRARIVDLVKERRAVRVSSLSEDLGVSGM
                     TIRRDLERLEVEGVLVRTHGGAVLKRHISEETFYGEREAASAEEKRRIAEHAAALIEP
                     GETVFLSSGTTAAQVLRHIDPSLEARIVTHNVGALQEARGLRVEVVLVGGLYRPRSNA
                     VSGPLALEHISRFHAGKMLIGVDGFDLEEGLTTPTMGMAGVERAMIAQTRGEVIALAD
                     ASKIGVVADVVICGLDQVDTVMVDDGADKSMREEIVRMGLRCIVV"
     gene            complement(10040..11107)
                     /locus_tag="VFZ86_11360"
     CDS             complement(10040..11107)
                     /locus_tag="VFZ86_11360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642918.1"
                     /translation="MPDRGREAPSERPRAACLRGGALEDHHPIRRLALAALVAVATLA
                     AAGALAVPASARAGELRQNRDRAEAIAAEIAALDAQIGTSVTRYSRATRALEAVRTQL
                     QGNRRLQRLAYRQLGLARATLAARAVAMYKHDDVSPMDAVFGAADLSDLVTQLTMVRT
                     IARCDRDVVHTIETTKRELSDRAVKLAADERTAEKLVVTCERELGTIRSRLDERRAAL
                     AGVRSDIRRLVTAAQKASPDPVATVEPPAAAEGGVSDGSGPWWPLIQSAAAANGVSAR
                     GMYRLMMIESGGSAGVVGPGGYHGLFQYAPSTWRGSWNPWRSASISDGAAQIKATALA
                     IRQGNGHAWWDPSYTWAFQGD"
     gene            11387..12529
                     /locus_tag="VFZ86_11365"
     CDS             11387..12529
                     /locus_tag="VFZ86_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012834466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5642919.1"
                     /translation="MQAIAVKEFRELRRDRRTLAMLFLLPFLFLVVFGYAASFDIDDV
                     PTAVAGPGAAAAAGLLPETFDVVSTDEDGDRAAAQDLLRRGEAVVAVVTPGTGGGAAT
                     VFVDGTELFAAQASLRRLAELQAPDPPGGSGGQSPLAGAGASTVQVDVLFNPELRTAV
                     IMIPGLCGIILVFVGTIATALGVVRERQSGTMEQLAVMPFRPRDVFLGKIAPYLLIAT
                     ADMVVVVVAGMLLFDVPFRGSVATFALGALLFLFVTLGTGVLISSVSQTQAQALQLAV
                     MTMVPQFLLSGLFFPLYSMPWAVRWIGYLLPLTWFIKVARGVMVRGTPIGALWLPLVV
                     LAVMAAVVFTVSTLRFRRELAPTGRSRGGEGDTQGAAAEPAAGAAA"
     gene            12526..14142
                     /locus_tag="VFZ86_11370"
     CDS             12526..14142
                     /locus_tag="VFZ86_11370"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5642920.1"
                     /translation="MSTATVAGAWGVRDLTVRYGGRVALDGVTLDVPAGSVTAVIGGD
                     GAGKTTLLRALAGVVEPAGGSVRRPERRRVGYVAGAAAVYGDLSVDENIAFVAGAYGL
                     TAAERDERMARLLAQTGLIGVGSRLAGRLSGGMRQKLAFALAMLHEPELLVLDEPTTG
                     VDPVSRAELWRLIAAAAAGGAAVLVSTTYLDEAGRAANVALLEDGRVRTGGAAVRRGE
                     EARAARAGSGSADRGRGPGVARGESLAVAAQAVRRYGELVAVGGVDLEVHPGEVVGLM
                     GANGAGKTTLLRLLLGLLRPTSGRVLLFGRPPSRQTRGRLGYVPQGLGLWEDLSVAEN
                     LSFSAQAFGAPPPALEPDLAAASATLVRDLPLGLRRRLAFAAALAHGPELLVLDEPTS
                     GVGLDARAALWETIHQAAVAGAGVLVTTHHLDEAGECDRLVLMAGGQVVAEGTLAGIV
                     GDGTAVAVRAVRWDQAFAALDAAGLPAALVGRDLRVPGGDLPAVRAALDGAGVPAHLA
                     VVPATFEETFVRLARAGQDRGSPDHERTTQ"
     gene            14139..15893
                     /locus_tag="VFZ86_11375"
     CDS             14139..15893
                     /locus_tag="VFZ86_11375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5642921.1"
                     /translation="MTDPVSPAPAPGGPPSADPGHPRKWWILIVVSFGMFMALLDVTI
                     VNIAMPAIITDLDTTVAQASWVLNSYNLVLAVFFLSMGRVADRYGQKRVFIFGLVTFT
                     IFSLLCGFAPNIEWLVAFRAGQGLGGAALLTISLAIVLGVFPKRQQGAAVGIWGALGT
                     AAAAVGPALGGVLVTYGDWSWIFFVNVPIGVVAVVVCAWVIPRGERKPAAEGGIDIPG
                     MVISGAGLFCLTLALVEGDTWGWTSATVIALFVAAAVSFPLFMWWETSTASPMFPVSL
                     LRIRSFTAANTAILFVGVAMGGTFLMLVIFLVSVLGFSELRAALAMTIMPVLALIVAP
                     NAGRLNDRIGPRYPAAAGAACFAIGLTLLAQLGGDTTLLDVMWRAAFIGVGMGLAMPT
                     LSAASMASLPPQVRGVGSGSLNTMRQVGFTVGVALLVAVFTHTVAQNARQATRQAAGV
                     IAAQPQLSAAEKGAYTAALIENARAAAGSGGGAARMTTSPLQGAPRPPGSSAAVFEEL
                     DSTLATLYRDVVAESFTWPFLVAALAALVAIIPALLTGRRLGEHEGHHEMTRSERIEA
                     AGRSPADGAGVPTVDDER"
     gene            15883..16530
                     /locus_tag="VFZ86_11380"
     CDS             15883..16530
                     /locus_tag="VFZ86_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012183498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX5642922.1"
                     /translation="MSAEARRSGRRQGESSSRADILAGARASFAEHGYDRATIRDIAR
                     RARVDPALVIHYFHSKEALFGEALELPVHPGEVFARGLAAGPGRLGATIIRTFLESWE
                     PPENRVRLMAMLRSALTNETAMDMMRDLLVREVFGPITEALGVPDAQLRATLVGSQLL
                     GLAVLRYVGRIEPLASASVDELVAAIGPTLQRYLTEDLGRGGAVDRGADAAPPVR"
     gene            complement(16523..17650)
                     /locus_tag="VFZ86_11385"
     CDS             complement(16523..17650)
                     /locus_tag="VFZ86_11385"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX5642923.1"
                     /translation="MTTPSFEQGPIRPPSEAHSLLVRVIRNCTWNRCTFCPVYKGAKS
                     SLRPVEEVLADVDAMAEAAEVLRRRGVHAVREGLVPPEAYQVSLFLGDGARTVFLQDA
                     DPCAVKPDKLAAVIRRVRERFPTVERVTTYGRASTLARRAPEHLALLADAGLTRVHLG
                     LESGADEVLMAIDKGCTGGDLIAAGEKVLGAGLELCFYLMPGLGGRADAAAHVAGSAR
                     VISAVARSAPAGNPLVVRLRTAAVVPSTPLGARAAAGAFVLPDDVEVAQELRDLLEQV
                     GEARFELRSDHMLNLLQELEGSLPRDRERLTALLDAYLGWSRDDQARFAIGVRLGVFR
                     RLADYDDPTRRRALDRQFEGYEQPSADELLQAASALRSRFI"
     gene            complement(17658..20333)
                     /locus_tag="VFZ86_11390"
     CDS             complement(17658..20333)
                     /locus_tag="VFZ86_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX5642924.1"
                     /translation="MQLLDDQHWHALPDGEIVDLLETDAAEGLDRFAVEHRRAQFGLN
                     QLTPPKGPGPLRRFALQFTDPLVLILLVSAIVTIFLDEWVDAIVIFGVVLLNAIVGYL
                     QEAKAVAAIDALSRAMTAEATLLRAGEQLRLPATEIVPGDLVLVQAGDKVPADLRLLR
                     TRDLRVDESALTGESVPVEKDHSVVPRDTVLAERVDMAYASTLVTYGTGAGLVVGTGD
                     RTEVGRISQLIQSAHDLKTPLTRKIDQFSKVLLVVIVVLAALTFAIGLVRGEDLVEMF
                     RASIALAVAAIPEGLPAAVTVTLAIGVNRMAKRRAIIRKLPAVETLGSTMVICTDKTG
                     TLTQNEMTVQRIVAAGEESRVSGVGYGPEGDIVPGPSAAAREVLLAGLLCNDTALNLT
                     DGRYEVAGDPTEAALLTSAAKAGLVAAGEATAMPRLDAIPFESAYQYMATLHDAGERR
                     RVVYVKGAVERVLERCSAAVDGAGRPAPLDAAAVHAQVDALAAGGLRVLAFARGTPPD
                     GTDEIGHDDVAGGLTFLGLQAMMDPPRPAAIAAVEACHHAGISVKMITGDHAATAAAI
                     AHEIGIVGAADGALTGSEMAAVHDDEFIELADRTNVFARVTPEQKLRLVEALQSKGKV
                     VAMTGDGVNDAPALKQADIGVAMGITGTDVAKDAADMVLTDDDFASIEAAVEEGRGVF
                     DNLVKFIAYALPTNVGQGLVILAGVLLGTALPIEPLQILWINMITAVLLGLGLAFEPK
                     EPGIMDRHPRAPGAPIVSRGVLIRIVVSGLILLVSAFAVFEWALANDLGEEAARTSAV
                     NVFMSVQIFYLFACRSLRRSLFTYNPFGNRIILLGVAVVAGLQVLFTYAPFMNTAFDT
                     TAISGTEWLVILGIGVAVMVFMDLVGVLLRRLHID"
     gene            complement(20437..22983)
                     /locus_tag="VFZ86_11395"
     CDS             complement(20437..22983)
                     /locus_tag="VFZ86_11395"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX5642925.1"
                     /translation="MVRIAWRSLTAHKLRTFLTTLAILLGVAMISGTYVLTDQIDRGF
                     KQVFTDAYKGTDVTVTRQARFSGQLTGATEGLPEDMVRQVEAVDGVGEVFGYVSGMGA
                     VAVDGEVVETGGSPTLFFSYSPNDIAPPQYTEGRPPEQPGEVAVIEKLAQDKKLSPGS
                     PITVITDGGGHEATVSGVFRFAAQSSLGGSILIHTTLSDAQQWFDMQGRVSEIDVKAE
                     AGVSADELARRIRAALPEYTEVKTGAQAAADQTKALSDAIGAFLRPVLLSFGGIAVLV
                     GAFIIFNAFSMTVAQRRREFAMLRALGASRAQVLWSITGEAFVMGVLASVLGLFAGLG
                     VAAGVNYVFQAAGFDIPRSGLVMQPRTVVIALVVGIVVTVLSAVIPAQRATRVPPMAA
                     LQEGAVLPPSRFARFMPALAVVVAILGGLLIGGGMYGPGGTVVRLGTIAFGAVLVFLA
                     VAIASRYFVGPLAAWLGWPLQKLAPISGRLARDNSRRNPSRTALTAAALMIGLAVVVF
                     VAVLAQGLKSSFIDSYDRTVRADFVIASSNFMTIPTDTATRVQSVQGVETAVSLDAQQ
                     VQAENGSLPVVWGIDPVAIERVWSFDWISGGDDVLTELRLDGAIVEEQTASSLGVERG
                     GTIEVTTVEGEQATLRVLGVYRDPMMLNGIAVGTSGYNALFPKPQTFMVVAKAQPGAD
                     IAATQKEIETALASAPTAEVQTTEQYKEGTVDLVNQLLLLIYGLLALSVIISLFGIVN
                     TLVLAVYERTREIGLVRAIGMSRGQVRATVRYESVITSIIGAIMGIVVGIVFAWVVTT
                     RFAGQGITFSVPGVQLAVFLVLAVVVGVIAAILPARRAARIDILQAIHYE"
     gene            complement(22983..23720)
                     /locus_tag="VFZ86_11400"
     CDS             complement(22983..23720)
                     /locus_tag="VFZ86_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017936355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5642926.1"
                     /translation="MGLADAVVQARGIERDYGQGDALVRALAGVEIDLQRGAYTAIMG
                     PSGSGKSTLMHILAGLDRPTAGNVWIDGQEITTLKERALTQLRRDKIGFIFQTFNLLP
                     TLNAAENISLPLTIAGRKGDQAWVDELVETVGLGDRLTHKPAELSGGQQQRVAVARAL
                     ASRPSVLFADEPTGNLDSRTGEEVLQLLRHSRDVMGQTIVMVTHDAHAATYADRIVFL
                     KDGAIAHDCGRLGRDEIYDVIKSLEDA"
     gene            complement(23927..25315)
                     /locus_tag="VFZ86_11405"
     CDS             complement(23927..25315)
                     /locus_tag="VFZ86_11405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642927.1"
                     /translation="MREDRLLRVLRPSLALAVLAAALVLLGLAIAGPAGAATAAASPA
                     PTEPGVVVVTREDVVRFGEDIVVSKGSQVPTVVAFGGDVTVNGTVTDTVVAFGGYVVI
                     NGTVEKSVVAFGGDVKLGPKAIVGSDLAPTDSALVLMGGELVKAPGAQVTGRTEKIEG
                     ISWNGAGAWAARAWLENRLWGFSLAGWLVQTAFFLVLALVAAALMPQQLRGVHLRLGR
                     KPWASLGWGALLFFVVVPAILVVLVISLIGLLLVVPYALVVLLAYFFVTTGVASFLAQ
                     KVLTGFGGRENLMLAVTLGVVGTTVVSRIPFAGPLLVTVMMVFGAGAAALGVAEWRRG
                     KREEAAALAAANAAAAAAQGAAYPASAAALPAQAAAITPLVYTAPETPPAAPDVTAVA
                     PAVPAPETVVADVPPAVSAGEGIAEAPAAPAEPAAPVEPAAPASPTPETPGSGTPQND
                     VQGDVGTGPAQS"
     gene            complement(25342..26082)
                     /locus_tag="VFZ86_11410"
     CDS             complement(25342..26082)
                     /locus_tag="VFZ86_11410"
                     /inference="COORDINATES: protein motif:HMM:NF025168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing protein"
                     /protein_id="HEX5642928.1"
                     /translation="MNDSALVRFFGVVRSPRTWLGILFNWLAFPLGLFYFVFLVTGLS
                     LGIGLVVVWVGIPILLVVAGAWWLFGAFERLQAKHLLGADVPPAPREWEKVDGVWAKL
                     KAHFGCAATWKDLVYLLAKLPFGIVSTTLLVVGASMVFWFVSMPFFAVFDVPVINNTW
                     VPPLWFGILCLPLGVLVFFVALHVLNAWSWVCARWAEVMFRGPAQAPAMAPLVPAPPP
                     PPAPAPPVPAPTPAASAPAPPTDDETAS"
     gene            complement(26240..26920)
                     /locus_tag="VFZ86_11415"
     CDS             complement(26240..26920)
                     /locus_tag="VFZ86_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017625465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5642929.1"
                     /translation="MTVKIVLADDEQLVRSGLRLILETEDDIEVVGEANDGAEAVTLT
                     RRLDPDVVLMDVQMPVMNGIEATREIVALGRAESSRVVILTTFDLDEYVYEALKAGAS
                     GFLLKRTPAEDLVAGIRVVAEGEALLAPSVTQRLIETFRNRPEATPQRDASELDDLTD
                     REREVLVLVARGMTNGDIAAHLFLSEGTVKTHVKRIFAKMGLHDRTQAVILAYEVGLV
                     RPGESPAE"
     gene            complement(26958..28178)
                     /locus_tag="VFZ86_11420"
     CDS             complement(26958..28178)
                     /locus_tag="VFZ86_11420"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX5642930.1"
                     /translation="MERPADILAAPAGAAPAPPRRPRVLDPIPVSVFLALVFTAAGLV
                     ELLTRGSVPAGYHADGAALIGLTVASGLTLAVLWWSPLTSLVAATVLLCVQSLAGYEF
                     TQAAIWAVIVASFATVAFDHWKRAVAAGFVVSVGIVIVFVALPVLTWQSALSTWASLS
                     IVWVVAVIVRVYRGSIERAERRAALFAADREARAREAVTEERARMARELHDSVGHALN
                     VVVLHAGAAQRVIEKKPELAREALESIETAGRQALGDIERMLGILRAPEEEACCDVTP
                     GMGQLETLCDQVREAGLPVALTVTGAPRPLPASLDLTAYRIVQESLTNTLKHAGKTRA
                     TVTVRYEERALAIEVLDEGRGVTPATAGGGRGLLGMRERVATFRGELEAGPRPEGGFG
                     VRARLPLDGAGETM"
     gene            complement(28198..28556)
                     /gene="ssrA"
                     /locus_tag="VFZ86_11425"
     tmRNA           complement(28198..28556)
                     /gene="ssrA"
                     /locus_tag="VFZ86_11425"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(28710..29060)
                     /locus_tag="VFZ86_11430"
     CDS             complement(28710..29060)
                     /locus_tag="VFZ86_11430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642931.1"
                     /translation="MSEEQLECQECSAQCEKVVYPAACLAMNCRFLYAFKEDGDTFFG
                     CIEKVFPHEIDLKMFQEIERGKGGFGVVKVARQPLPQCSIAVQSCYATGEGPLCRNMY
                     FRRRDRREVQTVED"
     gene            29334..30722
                     /locus_tag="VFZ86_11435"
     CDS             29334..30722
                     /locus_tag="VFZ86_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009141725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEX5642932.1"
                     /translation="MDAVMLARIQFALTIGYHFIFVPISIGVGMMVVLAERRHYKSGI
                     AADRAASNFWIKLFTATFAIGVATGITMEFAFGTNWAEYSRFVGNIFGAPLAAEGLIA
                     FFLESTFLGILLFGRKRVSRGFYYASAWLVWIGALLSALWILIANSWMQTPAGYEIKD
                     GKAVLTSFWAAALNPSIGPRYFHTVSAALLTGCFVVAGVCAYYMLKKRHAQFARRTML
                     WAAAVGLILAISMPFIGHWGALVVAEYQPVKMAAFENIGDTQKNAPLYLFGWVNSAGD
                     TTGISIPSGLSLMLGLSPDHEVTGLNSVAAADRPPVQLTFQSYHLMIALGFLFILVFF
                     VAIVLHRLGRLERTRWFLWVLIGCIPLSLIAINMGWTAAEVGRQPWVVQGLMRTSDAV
                     SPLVSAGEIWATIGLFVLIYTVLFIAWLRIFLGIIKKGPEDPAEMLEAEKASAEPLSP
                     DTAGPAPAGAGR"
     gene            30727..>31289
                     /gene="cydB"
                     /locus_tag="VFZ86_11440"
     CDS             30727..>31289
                     /gene="cydB"
                     /locus_tag="VFZ86_11440"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009141726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEX5642933.1"
                     /translation="MTLANIWFFLVALLLTVYVILDGFDLGAGVLYPFLGKTDADKSV
                     IRAAIGPVWDGNEVWLITGAGAIFAAFPMVYAMTFSGFYLAIMLVLFGLILRAVSLEF
                     RHRDSSWSGVWDAAFFVGSLVPSVLVGVALGNVIRGVPMDAQGNYIGTFWELLNPYSL
                     LVGVTGLFLLVQHGAAWLAVKAEGELHA"
BASE COUNT         4553 a        10967 c        10914 g         4855 t
ORIGIN      
        1 gccggcgcct ggatcacttc gccgcgaagg cgggtcccgt cggcttattc gaccgggatc
       61 tccttggcga cggcctcgag ctccatgccc tgcttcttgc ggaccatcca cgcgatcagc
      121 cagatcacga cgccggccag atagaggacg cccatgaaga cggctgactc ccacaggttg
      181 atgtagtaga cgccgtcagg atcccacacg aacatgaaga tgttgaagac catgaaggcg
      241 cagtagaggg ccgcgatgat ggccatccac gggatgccgg cgatgcccga cttgggcagg
      301 gcgctgttct cccagatccc gcgggcgcgc cacttcatga ccatgaaggc gagcccgctg
      361 aagaggaaca tgatgatgat gccgaacgtg gcatcgtagg tgaagagctt gacgatcgtg
      421 accccgcgct cctccacgac gggggtgtac gcgtagagcg ccgccacgat gatcgacggg
      481 atcgtcatga gcagcagggc gaccgtcggc acgccgccgg aggtcacctt ggccgcccgc
      541 tcgggcagga tgcggtcgaa cgcggacgcg aagatcatgc gcgtcgacga gaggaagagc
      601 gtgccgaacc agccgaagag cagcaggctg atggcggcca ccagaacgac ctggaaggcc
      661 gcgttgggga cgatccagct gatcatcgcc gtcggcgaga tgtaatcgcc gccgaggggc
      721 gagatgcccc accagaacgc cgccatggtg cccatgaagg cctggtagcc gaccttccag
      781 gtcatcagcc cgaggaagga cagggcgatg atcgtcggca cgagaagccc gccgagcatc
      841 tggtagatgt tcttgcggaa gtccttggcc ccgcggacct cgccgtacaa ggtggcgccc
      901 cagttcggcc acgccatcca gaacagcatg aacgggatga tggtccaggt ggcccagctc
      961 aggccgccgc tgaacgtgga ggtcggcatg cccgtgccgg cgccgacggc ctcgatggcc
     1021 tcgaacgcgc ccgcgtgagt ctcgccctcg acgggcacca gagcaccggc gtcgtcgtag
     1081 acgaacaggc cggcctcatc ctgctcgtac acggtgaggt cgagcagcgc cgcgtcgccg
     1141 ccgtagtact tctcggcggc ctggttgaac ttctccttga aggagtcgcc ggaggtcacc
     1201 agcagcagga tgccgcagat gatgacgccg gcgaggccga tccacatggc ccacttctgg
     1261 aagttggcgt aggtcttcat gccgacggcg accaggatgg aggcgatcgc gatgaccaca
     1321 agtgacgcca cgaagatgcc cgtcgccgtg ccgagccagt cggccgcccc atggaggccg
     1381 aggatacgga agatcggctt gaccgtggtc tgcacgccca tgtcggcgta gatcggcgtc
     1441 cagagccaca ggatgaacca ccagcccacc ccggcgatca cgaagccgat ggagctgtgg
     1501 aagatgcgtg tgatccagac gtagtctcca cccgcgcgcg gcatcgccga gatcaggcct
     1561 gcgtacacga tgatctcgag gacgatgaag atcgcagtga tgacgatcgc ccagaacagg
     1621 ctgccgcccg ggatgaacgt ggcgtacgcg aacgaccaga tcgcgaggaa caggttcacg
     1681 gagaagaacg aataccggaa cccgtccatc gacgaccagc ccttcaccag gccactcgcc
     1741 ttgcgcacga acagagtctg ctcgcccgtc ttgctagcgt cagtcgccat cagctaagtc
     1801 cccctttgtc cattgtcgcc cggcctgcca gacgtgcggg ccgggccctc cctgccgtcc
     1861 tccgagtggc cctcacccgg aggtcagcct gtagcgcttc agcttgctct tgccatcgag
     1921 gtcgaccacg acgcccgcga ccatgccttc gacgtacgag ctgcccgggt tgatggcgag
     1981 cgtgcggccg atacggaccg ccgcccggct ctcgtggatg tgcccgtgca gcgacaggat
     2041 gggctggacc tcttcgatca cctccttgac ggcggtggaa ccgacgtgcg cagagtcctg
     2101 gccctggacg gtgagcgtct cccagtcgat cttcggggcc acgtcgagct gcgtgtcgta
     2161 gggcggcgcg tgcaggccga gcaccaggcg ctcgggcggc accgtcgcct gcttgacgat
     2221 gcccatgatg cgctccttga gcttgggctc gtcctcttcg cgcggggtgt cccacggcgt
     2281 gaggttggtc cagccgctgg acgccagctg gtacccctcc gggaggtcga ggacgttgcc
     2341 ctcgcccacc tgcatgtgct tggacgtcgc gaggatctcg tcgatgtccc agcggtcgtc
     2401 gttgcccggg cagacgatgc actggatgcc gctgccctcg agcttcgcgt ccgcccactc
     2461 gacccagtcc gccacggtct tgcgcatctg ctcgtggaac agctcctcga gcttgtcctc
     2521 gtccttcgtg tactcgtcgc gctcctcggg cgtcagggcc acggggtagt agccgtggtc
     2581 cttgatcaga cgctccgcct tggcgaggtc ttcggcgccg ttcagatcgt gcgtgatgtc
     2641 gagcatgtgg tagcgccact gctcggggcc ggtctggacg atcgggacga tcgccttgcc
     2701 ggtcatgtcg ccaccaagga tgaggacgtc ggcctcgtag tgcttggccg agttgatgaa
     2761 cttgcgccag cagacttccg agccatggac gtccgtggcg aagaagagcc ttaccttcac
     2821 gccgcacccc ctttcgccgc gtacgcgggt cgaatcggta ctgtcactga gccgccagcc
     2881 acttcgtgta gtcggggtcg gcgatgccgc gctcggcacg tccgacgcgc tccatcgccc
     2941 attccatgta gtcgaagtcg atcttcgaca cggtgtgctg gatcatcgcc cagacgtccc
     3001 accacacggc ggagacgatc ttgtagagca tcatccggcc gaacagccgc tcgtccatct
     3061 cgcccaggta ggtctcgatg atgagccgct cctcctcgcg cgagtacgga tgctccatga
     3121 cgtggtcgcc gaggtcgaag tacgggtcgg tcatgccgcc gtactcccag tcgatgatcc
     3181 acatcctgcc gtcggcgtcg atgatgaagt tctccgagag caggtcgttc tggcaggcga
     3241 cgtccggtac ggggttccgt cccacagcct cctcgatctc gggcatccgc tcgaggagcg
     3301 ccgcgagccg ctcaggcatg ggggcgttga tgtcctgcgc caccttcgtg taccggcgca
     3361 gcatgtcgaa gatcgcgatc ttgttgctga agacggcctt gtcgtggtac acgcggactg
     3421 cctcgacggc ctgcttgatg cgctccgggt gcccggccat cgtgtcgggg tgcatgacct
     3481 cgccgtcgat gaagtcgatg accatggcgt tctccggctc gatgacgtga gcgacccggg
     3541 gagacgtgcc gatcctggcc acctcgagca tgcacgcgcg ctcgatgtcg cggtcgatga
     3601 acatgtcggt acccgcgccg gggacgcgca gcacgtactt gccgccgccc ggattgcccc
     3661 agccaccgtt cacccagacg atgtagttgt ggttggtgat accgccgccg agctcctccc
     3721 agcggatctc cttgcccttc cagtcgtcga tgcgcgcaat cagctcgtcg ggcgaaaggt
     3781 ggtgagcctc tgttgccatg taccctccct ttcctacggg cggcgctgcg gccgcacggg
     3841 tgtcggcgtt accgaagagg cgcctgtcgc gcgccgagtg cgcggcgccg tccccgccgc
     3901 cgtgaggcgg gcggggacgg cgcgggaagt cgagcgacgt gggaggcgca agctcacgaa
     3961 acggggacct cgaggcccat gccggcgagc tcctcgcgga cctcgtccat ggccttctgg
     4021 tagtcggcgt tcacgatccg cttggtcctc atgtcgtaga cctcctggaa cgtgaggccc
     4081 ttgtccgggt ccttgagctt gtcgccgaag agcgggaaca gcttctgggc gatgggctcc
     4141 gcctgggcgc gcgtgagctt ggaggcggcg agcccgatct ccgcgtggaa cttgtggtcg
     4201 tccgggacga gggagtccga ctggacggcc attgcggggt gacccgagaa gacgtttcca
     4261 ccagacgtca cgaccgcgag catatagcag gccatttcgt aatggtactg cttggtgccc
     4321 gagccgccgc ccaggtaggg aaggttgacc atcggatact gcatgttgcg gccgatggcg
     4381 gcgttggaca tggcgatcgc ccagatcagc ggcttggtcg acgagaggtg cagcttgctg
     4441 tgcagcgggg cgttgtagtg atagctgccg cggaagacca ggttgcccat gatcatgtac
     4501 gcggtggcgg tgacggccac gccctcggcg ccgcccgcgt agccgccgaa gagcggcgtg
     4561 ctggtggagc cgatgttggc gctgatgctg ttgtagaagg cgaccttctg cagcgcctcg
     4621 tacccgacga ccatctcgga gaggaagtcg atcaggaagc cgtcgctcgg gcgcaggccg
     4681 aagtccggat acgacgccgc gaacatcgac gccggctggg aggcggcgga gatgccgttg
     4741 atgatcggca tgcccgcgcg gccggagcgg cggatgccct cgcgcgccag gcgcacggtc
     4801 ttgcaggccg agaggatctc ctgcggcgag cgcacgcgcg agtccttgcc gcgcaggtgc
     4861 aggatcgagg ggacgctcac cgagtcgacg atgttgtagg acgccatctt ctcgacggtg
     4921 tcgacgaaga ccccctcgtc ggtggtgtag atgccgccgc cgacgtgcac ccacgggcgg
     4981 tcgtcggtgg tgtcgggacg acggccgcgc atgacgttgc gctcgcgccc ctcgccgaac
     5041 gtggtcgcct tcggcgcctg ctcgcaggcg acgacgatct cgtcacgcgt gaactgcatg
     5101 acgcggttgg tctcggggca gtaggcgccc gcctccgcta tgagatcgac cgcggcctcg
     5161 aacacggcgt ccgtcagcgt gtcgtcggcg ttgatgatgg tctctttgtc ccacttcacc
     5221 ttgtggcggg cgatcacctc ggtgagcttg gggacgtagc gcttcatgta gtagtcggtc
     5281 tcggaggtga gcgggccggt ctcggcgcga tcgaagacct cgataaggtc gatcatgtcg
     5341 tgctcctcgt agtcaggggg tcgtcgggcc gggtcaggag accagcttgg tggacagggt
     5401 cacggcctcg tacgccgtcg ccgcccagcc gtcggcgccg atcgtgtccg cccactcctt
     5461 ggtggtcgcg ccgccgccga tgatgacctt gaacttgtcg cgcgcgccct ggtccctcag
     5521 cgcctcgatc acgtccttct ggccgacgag cgtcgtggtc agcagggccg acatggcgat
     5581 gatgtccgcg tcgacttcct tggccttgtc gacgaactgg aacaggtcga cgtcgatgcc
     5641 caggtcccac acctcgaagc cggacgtctc gtagaggagc ttgaggatgt tcttgccgat
     5701 ggagtggagg tcgcccttga cggtgccgag gacgatcttg cccttcttct caccctccgc
     5761 gccctgctcc ttgatcttgg gctcgaggat caccatcgcc gactggaagg cgagggcggc
     5821 ctgcatcagc tcggggagga agatgtcctc gcactcgaac tgctcgccga tcacgttgag
     5881 gcccggcagc aggccctggt cgatgatggc tttggcctcg acgccgtcgt ccagcgcggc
     5941 gttgacggcg gcgaggacct ggtcctcgtc ctggtcgatg atcgactgct tcagcttgtc
     6001 gaagtgttct gcgtccgcca tggaatgcac ctcttggtcc ggttgaacgt tgccggccgc
     6061 cctccggcga ccgctcgcgc gtgggtactt ctgctcagct cgtcgtgtca gcctccagcg
     6121 gtgtcgcgtc gccggcgacg aggccggcga agtgctccct gatcctggcg tccagctcgc
     6181 cgtcccagac ggcctcgttg ggctgcgcca ggatgcggcg cgcctcggcg agagcgcggt
     6241 cgttgatggt cggccggccg gcgtcctccc actgctggcg catgtcgcgg ttggcgagcc
     6301 tgggcagcac ggccgtggtc ttcatgcgcg ccttggtgtg cttgcggccc atgtagctgc
     6361 cgccgggtcc caccgcggcg atctcgtcga gcgcgaggtt ctcctcgctg aactccaggc
     6421 cgcggtaggc gcgcttgtcc atgatcgcga tctcgttgtc gacgacggcc ttggcgaagt
     6481 cgaacgccat gagcgagctg tagaggccgc ccatgttgag cagcgtcgcg ccggcggaca
     6541 cgccgagcac cgtgttcatg ccggtctcgt agccggcctg ggcgtcgttg gagtgcgcgt
     6601 tggtgaggcc gatgtagccg cccgagggga ccccgtagaa gcgcgccagc tgcgcctgcg
     6661 cggcgagcag gatgcccgtc tcgatggcgc ccggcgcgta cgcgccggta cgcatgtcgg
     6721 cgacggtggg caagctcgcg tagatgatcg gcgtgccctc cttgaccatc tgctggaaca
     6781 cggtgatggc gaggaactcg gcgttgccga ccaccagcgt gcccatgagc gtcatcggcg
     6841 aggtgaggcc ggcgttcggc accacggtcg tgtagacggg caggccgcgc tcggtgagcc
     6901 acatgacggc ctcggtcgag tccacgtcca tggtgagcgg gctgaccatc gggcagtagt
     6961 ggtggttgat gcacgggcgc tcggcgtagg cctccctgcc gccggcgatg agctccccga
     7021 gctccacgac ctgctgcagg tcgctcatgt tcggggtgtt gctgcgcacc ggcttgaggc
     7081 agttcttgag cgccgggtag aaccggctca ggctgaactg cccctcgggc gcgtcgtcgg
     7141 ccagcgtcga gatgctgaag acgtcgaagc cgggcagctc gttgatcagg tgggcgatgt
     7201 tggcgatgtc cgccgacgtt gcccggcgct cgacgccggt gaccgggtcg accacgttcg
     7261 gcgccgacga gccggtcacc acgatggggc cgtcgtcggg gatggtgcga tcgtactgcg
     7321 ggtcgcggcc gcggaacgtg aagctcggcg gcgccagctt gcggtacttc tcgacgatct
     7381 ccgccgggaa ccgcaccagg ccggtctcgt cgtccacgat gcagccgtgc ttcttgaaga
     7441 cctcgcgcgc cggcgcgaag tgggccagga tgcccaccgt ctcgaggacc tcgagtgagg
     7501 cctcgtgcgt acgcctgacc tccgcctccg tgagcagctc gacgtacctc atggtcgttc
     7561 cccctctctc tctgcgaccc cgcgggcggg cgccttggcg cgtgcggcca tggcggccag
     7621 ctcgtcgagg tgggcctcga tgaaatcgac gaacgccacg ttgtgctcga ggaagaagag
     7681 gtattcgtcg tcgttcgggt cgcggtcgat gtagtacgcg acgatgtccc agctgaccac
     7741 gtacagcgcg gcgatcgcca gcatgcgcgg cagcacccgg agctcgccgt cggtgaccgg
     7801 gccgggcgtc gcggagcgag acgcctcgtc ttgataggcc ttgaggaaga tctcggcctt
     7861 gtccatgcgc agctggccgt cgtccttgtc ctcccatgag gtgcagaagt agacgatgcc
     7921 gatggcgatg tcgaagatgc ggtagtcgag cttggtccag tcgaagtcga acaggccgac
     7981 cccgtgctcg tcgcgccact tgaggttgcc ggggtggtag tcgttgaacg tggggcagcg
     8041 cggcagcccc tcgagctcgg gagccagcgt gaggccccgg tcgatcgccg cgaggatctc
     8101 cggcagcctg ccgaggaagt agtggtcgta cttggtgccc ttcgtcccgg ccgcgtagcc
     8161 gttgaacgtg ccgggcatgg tcgcgaggta ctccatgatg ggcggttgct cgcggaacag
     8221 ctcgccgggc tcgaagtcgt gggcggcgtg atggaagcgc gcgaggatgc gggcggcgtc
     8281 ctcgtactcc gtgtccgtgc agcggttctc ggtccaggtg tacttgtcct cgccgccgag
     8341 catctcgtac accgcgaaga agcgcgtgac gcgctcgtcg ccgaggagct cgtcgcgcgt
     8401 caccagcgtg tcgccgtgcc tggtctcgaa cacgcgcgcg ggaagctcga agccgttctt
     8461 gcggatgtgg ccgacgagcg cgtgctcgta gcggatctcg cgctcgaccg tgccgtactt
     8521 gtacttgcgc acgaagtacc gcgcctcacc gtcgccgtcc ctggtgacga cggcgaagct
     8581 caggttctcg aagccgccga agatctgctc gacgcggagc acgtcgccga gatcgtagtc
     8641 ggtggccacg acctccgcga gctggccgct gaggaactcg acctgcagcg cgtcgcgcag
     8701 ggtctgggca tcggcggcga ggcgtatcgc cgtctcgaag agctcgttcg ccttgcttac
     8761 gtccactcca cctcagttcg gtcggcgcgt cgcgctcggc gcgcgcgcaa cattcagacg
     8821 acgatgcatc gcagccccat gcgcacgatc tcctcgcgca tgctcttgtc ggcgccgtcg
     8881 tcgaccatga ccgtgtcgac ctggtcgagg ccacagatga cgacgtcggc caccacgccg
     8941 atcttggaag cgtcggcaag ggcgatgacc tcgccgcgcg tctgcgcgat catggcgcgc
     9001 tcgacgccgg ccatgcccat ggtaggagtc gtgagaccct cctcgaggtc gaacccgtcc
     9061 acgccgatga gcatcttgcc ggcgtggaag cggctgatgt gctcgagcgc gaggggaccg
     9121 ctgacggcgt tggaccgcgg ccggtagagg ccccccacga ggacgacctc gacgcgcaga
     9181 ccccgagcct cctggagcgc accgacgttg tgcgtgacga tgcgcgcctc gagtgagggg
     9241 tcgatgtgcc ggagcacctg cgccgccgtg gtgccggagc tgaggaacac cgtctcccca
     9301 ggctcgatga gcgcggcggc gtgctcggcg atgcggcgct tctcctcggc gctggccgcc
     9361 tcgcgctcgc cgtagaacgt ctcctcggag atgtgccgct tgaggaccgc gccgccgtgc
     9421 gtgcgcacga ggacaccttc gacctcgagc cgctcgaggt ctctgcggat ggtcatcccc
     9481 gagacaccca ggtcttcgct gagcgacgac accctgaccg ctcgccgttc cttgaccagg
     9541 tcgacgatgc gggcgcgccg ctccgcgggg atcatctcgt ggacttccgg gacttctgac
     9601 gacacaggcg gttcccccag tacgtagcgc ctcacggccc ggcaagaccg aacaaagtcg
     9661 aacacatctt aaacgtgccc cactcagtgt caagcgctgg acggcgttca ggcacgagaa
     9721 gagtgcacgg aagagaacgt gagacctgac ggccgggcgc tgaactgccc acatctacca
     9781 tcctgcgccg gaggctccca ctgttcgcgt cccacggcgc ccggccgttc ccgtgcctgc
     9841 acgggtggct cgcatcggct ctcaccgatg gagaggtccc gacgatgcac tgcccgatga
     9901 cgtgaaatcc gagcactttc gagcaatacc gaacgaagtc taacgcatcc cggcacgccg
     9961 tcaacccccg gggagcacgc gcgggacccg gtgcgagagc agtgcggatg cgcggccggc
    10021 gggcggaccc cgccgcgact cagtcgccct ggaaggccca ggtgtaggag ggatcccacc
    10081 aggcgtgacc gttgccctgg cggatggcga gcgccgtcgc cttgatctgc gccgcgccgt
    10141 cgctgatgct cgccgaccgc caggggttcc agctccctct ccaggtggag ggtgcgtact
    10201 ggaagagtcc gtgatacccc ccgggcccga cgacgccggc cgaccccccc gactcgatca
    10261 tcatgaggcg gtacatgccg cgggcgctca caccgttggc ggcggctgcg ctctggatga
    10321 gcggccacca cggcccggaa ccgtcactga cgccaccctc ggccgcggcc ggcggctcga
    10381 cggtcgccac ggggtcggga gaggccttct gggcggcggt cacgaggcgg cggatgtcgg
    10441 agcgcacgcc tgccagcgcc gcccgtcgct cgtcgaggcg ggagcggatg gtaccgagct
    10501 cgcgctcgca ggtcacgacg agcttctccg cggtgcgctc gtcggccgcg agcttgacgg
    10561 ccctgtccga gagctcgcgc ttggtcgtct cgatcgtgtg cacgacgtct ctgtcgcagc
    10621 gggcgatggt gcgcaccatc gtcagctggg tgaccagatc gctcaggtcg gccgcgccga
    10681 acaccgcgtc catcgggctc acgtcgtcgt gcttgtacat ggcgaccgcc cgcgccgcga
    10741 gggtcgcccg cgccagcccc agttgcctgt acgccagacg ctgcagccgg cggttcccct
    10801 gcagttgcgt gcggaccgcc tcgagcgcgc gcgtcgcgcg cgagtaccgc gtcacggagg
    10861 tgcctatctg cgcatccagg gcggcgatct ccgcggcgat ggcctcggcc ctgtcgcggt
    10921 tctgccggag ttctcctgcc cgtgccgaag cgggtacggc gagcgccccg gcggccgcga
    10981 gcgtcgcgac ggcgaccagg gcggccagcg ccaggcgacg tatagggtga tggtcttcca
    11041 gtgcgcctcc tcggaggcag gcggcccggg gccgctcgct cggcgcctcg cggccgcggt
    11101 caggcacggc cgaaaggtag gggacgaaac ctaccactcc cgcgcactcg tcttcaagcg
    11161 caggaatcca agcggccgcg acgacgtgtc gctttcgcac aattcccgca cgcagtccgc
    11221 cggaatcggc aggacacgtg gcgcagtgcg acggttccgg cagaccttcg tcaccagagc
    11281 ggtcgcgtga tcgtctgtgg tgaaccgtga gcattgaccg cgctttcaac acgtggtgaa
    11341 atcaacgtct gttgaagagc atctgacacc gacggaggga ggcggcgtgc aagccatcgc
    11401 agtcaaggag ttccgggaac tgcgacgaga ccgccgcacc ctcgcgatgc tgttcctgct
    11461 gccgttcctg ttcctcgtgg tgttcggcta cgccgccagc ttcgacatcg acgacgtgcc
    11521 gacggcggtc gccggaccgg gagctgccgc ggcggccggg ctgctgcccg agacgttcga
    11581 cgtcgtgagc acggacgagg acggcgaccg cgccgccgcg caggacctgc tgcggcgcgg
    11641 cgaggccgtg gtcgccgtcg tcacgcccgg cacgggcggc ggagcggcga ccgtcttcgt
    11701 cgacgggacg gagctcttcg ccgcgcaggc gtccctgcgt cgcctcgcgg agctccaggc
    11761 gccggatccg ccgggcggct ccggaggaca gagtcccctt gccggcgccg gcgcttccac
    11821 ggtgcaggtg gacgtgctct tcaacccgga actgcgtacg gcggtcatca tgatccccgg
    11881 cctctgcggg atcatcctcg tgttcgtcgg caccatcgcc acggcgctcg gcgtggtgcg
    11941 cgaacggcag agcggcacga tggaacagct cgccgtgatg ccgttccggc cgcgcgacgt
    12001 gttcctcggc aagatcgccc cctacctgct catcgccacc gccgacatgg tcgtcgtcgt
    12061 ggtggcgggc atgctgctgt tcgacgtccc cttccgcggg tcggtcgcga ccttcgcgct
    12121 cggggcgctg ctcttcctct tcgtcacgct cggcaccggg gtgctcatct cgagcgtctc
    12181 ccagacccag gcacaggcac tgcagctggc ggtcatgacc atggtgccgc agttcctgct
    12241 gagcggcctc ttcttcccgc tgtactcgat gccctgggcc gtgcgctgga tcgggtacct
    12301 gctgccgctc acctggttca tcaaggtggc gcggggcgtg atggtgcgcg gcacaccgat
    12361 cggagcgctc tggctgccgc tggtggtgct ggcggtgatg gccgcggtgg tgttcacggt
    12421 ctcgacgctg cggttccggc gcgagctcgc gcccacgggg cgctccaggg gcggcgaagg
    12481 cgacacgcag ggcgcggccg ccgagccggc ggccggggcg gccgcgtgag cacggcgacc
    12541 gtagccggcg cctggggcgt gcgcgacctg accgtccgct acggcgggcg agtggcgctc
    12601 gacggcgtga cgctggacgt gcccgccggc tcggtgaccg ccgtcatcgg cggggacggc
    12661 gccggcaaga cgacgctgct gcgggcactg gccggagtgg tggagccggc gggcgggagc
    12721 gtgcgccggc cggagcgccg ccgggtgggc tacgtcgccg gcgccgcagc cgtctacggc
    12781 gacctcagcg tcgacgagaa catcgccttc gtcgccggcg cctacggcct caccgccgcg
    12841 gagcgcgatg agcgcatggc gcgcctgctg gcgcagacag ggctcatcgg cgtcggctcg
    12901 cggctcgcgg gcaggctctc gggcggcatg cgccagaagc tggccttcgc gctggccatg
    12961 ctgcacgagc ccgagctgct cgtcctcgac gagccgacca ccggtgtgga tccggtcagc
    13021 cgcgccgagc tctggcggct gatcgccgcg gccgccgcgg gcggggccgc ggtgctcgtc
    13081 tccaccacgt atctcgacga ggccgggcgg gccgcgaacg tcgcgctgct cgaggacggc
    13141 cgcgtgcgca ccggcggcgc ggccgtgcgg cgcggcgagg aggcgcgtgc ggcgcgagcc
    13201 gggagcggca gcgcagaccg cggccgcggg cccggcgtcg cgcggggcga gtcactcgcc
    13261 gtcgccgcgc aagccgtacg ccggtacggc gagctcgtgg ccgtcggcgg cgtggacctc
    13321 gaggtgcacc ccggcgaggt ggtcgggctc atgggagcca acggagccgg caagacgacg
    13381 ctgctgcggc tcctcctcgg cctgctccgc cccacgtccg ggcgggtgct gctgttcggc
    13441 cgcccgccct cgcgacagac gcgcgggcgg ctcggctacg tcccgcaggg actgggactc
    13501 tgggaggacc tgagcgtcgc cgagaacctc tcgttctcgg cccaggcctt cggcgctcct
    13561 ccgccggcgc tcgagcccga cctcgcggcg gcctcggcca cgctcgtccg cgacctgccg
    13621 ctcggcctgc gccggcgcct cgccttcgcc gccgcgctcg cgcacgggcc ggagctgctc
    13681 gtgctggacg agccgacctc cggcgtgggg ctcgacgccc gggcggcgct ctgggagacg
    13741 atccaccagg cggcggtcgc cggcgcaggt gtcctggtca ccacccacca cctcgacgag
    13801 gccggcgagt gcgaccgcct ggtgctgatg gccggcgggc aagtcgtcgc cgagggcacg
    13861 ctcgccggca tcgtcggcga cggcaccgcc gtcgccgtgc gggccgtgcg ctgggaccag
    13921 gcgttcgcgg cgctcgacgc ggccggcctg cccgccgccc tcgtgggacg cgatcttcgc
    13981 gtgccgggcg gcgacctgcc ggccgtccgc gccgcgctcg acggcgccgg cgtgcccgcc
    14041 cacctcgccg tcgtgcccgc caccttcgaa gagacgttcg tccgcctcgc gcgcgcgggg
    14101 caggaccgcg gatcgccgga ccacgaaagg accacccagt gaccgatccc gtctcccccg
    14161 cccctgcacc cggcggccct ccgtccgcag acccgggtca cccgcgcaag tggtggatcc
    14221 tcatcgtcgt cagcttcggc atgttcatgg ccctgctgga cgtcaccatc gtcaacatcg
    14281 cgatgccggc gatcatcacg gacctggaca ccaccgtcgc ccaggcgtcg tgggtgctca
    14341 actcctacaa cctcgtgctc gccgtcttct tcctctccat gggccgggtc gccgaccgct
    14401 acgggcagaa gcgcgtcttc atcttcggcc tcgtgacgtt cacgatcttc tcgctgctct
    14461 gcggcttcgc cccgaacatc gagtggctgg tcgccttccg cgccggccaa ggcctgggcg
    14521 gcgcggcgct cctcaccatc tcgctggcga tcgtgctcgg cgtgttcccc aagcggcagc
    14581 agggcgcggc cgtgggcatc tggggcgccc tgggcaccgc cgccgccgcc gtcggccccg
    14641 ccctcggcgg cgtgctcgtc acctacggcg actggtcgtg gatcttcttc gtcaacgtcc
    14701 ccatcggcgt cgtcgccgtc gtcgtctgcg cctgggtgat cccgcgcgga gagcgtaagc
    14761 cggccgccga gggcggcatc gacatcccgg gcatggtcat ctccggcgcc ggcctgttct
    14821 gcctgacgct ggcgctcgtg gagggtgaca cctggggctg gacgtcggcg acggtcatcg
    14881 cgttgttcgt ggccgcggcg gtgagcttcc cgctgttcat gtggtgggag acctccacgg
    14941 cgtcgccgat gttcccggtg agcctgctgc gcatccgctc gttcaccgcc gccaacacgg
    15001 ccatcctgtt cgtgggcgtg gcgatgggtg gcacgttcct catgctcgtc atcttcctgg
    15061 tctccgtgct cgggttctcc gagcttcgtg ccgcgctcgc catgacgatc atgccggtgc
    15121 tcgcgctgat cgtcgccccc aacgccggcc gcctgaacga ccgcatcggc ccccgctacc
    15181 cggccgcggc cggggcggcg tgcttcgcca tcgggctcac gctgctggcg cagctcggcg
    15241 gcgacacgac gctgctggac gtgatgtggc gtgcggcctt catcggcgtc ggcatgggcc
    15301 tggccatgcc gaccctctcg gccgcctcca tggcctccct gccgccgcag gtccgcggcg
    15361 tgggctcagg gtccctgaac accatgcgtc aggtcggctt cacggtcggc gtcgccctgc
    15421 tcgtcgccgt cttcacgcac accgtggcgc agaacgcccg gcaggccacg cgtcaggccg
    15481 ccggggtgat cgccgcgcag ccgcagctga gcgcggcgga gaagggcgcc tacacggccg
    15541 cgctcatcga gaacgccagg gccgcggccg gcagcggcgg cggcgcggcc cggatgacca
    15601 cctcgcctct ccagggcgcg ccgcggccgc ccggctcctc ggcggcggtc ttcgaggagc
    15661 tcgacagcac cctcgcgacg ctgtatcgcg atgtcgtcgc cgagtcgttc acgtggccgt
    15721 tcctcgtggc ggcgctcgcg gctctggtcg ccatcatccc tgcgctgctc acgggccgaa
    15781 gactgggcga gcacgaggga caccacgaga tgacacgttc cgagcgtatc gaagccgccg
    15841 gtcggtcccc ggcggacggg gctggtgtgc cgacggtgga cgatgagcgc tgaggcgcgc
    15901 aggagcggcc ggcgacaggg cgagagctcg tcgcgggcgg acatcctcgc gggcgcccgg
    15961 gcgtcgttcg cggagcacgg ctacgaccgc gccaccatcc gggacatcgc gaggcgcgcg
    16021 agggtggacc ccgccctggt catccactac ttccactcca aggaggcgct cttcggcgag
    16081 gcgctcgaac tgcccgtgca cccgggcgag gtcttcgccc gcggcctggc cgccggcccc
    16141 ggccggctgg gcgccaccat catccgcacg ttcctcgagt cgtgggagcc gcccgagaac
    16201 agggtgcgcc tcatggccat gctgcgctcg gcgctcacca acgagacggc gatggacatg
    16261 atgcgcgacc ttctcgtgcg cgaggtgttc ggccccatca cggaggcgct cggcgtgccc
    16321 gacgcgcagc tccgcgcgac gctcgtcggc tcgcagttgc tcggcctggc cgtcctgcgc
    16381 tacgtcgggc gcatcgagcc gctggcctcg gcgtcggtcg acgagctggt cgcggcgatc
    16441 ggaccgacgt tgcagcgcta cctgacggag gacctcggac gaggcggcgc cgtggatcgc
    16501 ggcgcggacg cggcgccgcc ggtcagatga agcgcgagcg gagcgccgac gctgcctgca
    16561 gcagttcgtc ggcgctcggc tgctcatagc cctcgaactg ccggtcgagc gcccggcggc
    16621 gcgtggggtc gtcgtagtcg gccaggcgcc ggaagacgcc gagccgcacg ccgatcgcga
    16681 agcgcgcctg gtcgtcgcgc gaccacccca gatacgcgtc gagcagcgcc gtgaggcgct
    16741 cgcggtcgcg cggcagcgac ccttcgagct cctgcagaag gttgagcatg tggtcggagc
    16801 ggagctcgaa ccgggcctcg cccacctgct ccagcaggtc gcgcaactcc tgcgccacct
    16861 ccacgtcgtc aggcaggacg aacgcgcccg ccgcggcgcg ggcgccgagg ggcgtgctcg
    16921 gcacgacggc ggccgtgcgc aggcgcacga ccagcgggtt gccggcgggg gcgctccgcg
    16981 ccaccgcgct gatcacgcgg gctgagcccg cgacgtgcgc ggcggcgtcc gcacgcccgc
    17041 ccagccccgg catgaggtag aagcacagct cgaggccggc gccgagcacc ttctcgccgg
    17101 ccgcgatcag gtcgccgccg gtgcatccct tgtcgatggc catgagcacc tcgtcggctc
    17161 ccgactcgag gccgaggtgc acgcgcgtca gccccgcgtc ggcgagcagc gcgaggtgct
    17221 ccggcgcgcg ccgcgccagc gtgctcgcgc gcccgtacgt ggtcacgcgc tcgaccgtcg
    17281 ggaagcgctc gcggacgcgg cggatgaccg ccgcgagctt gtccggcttg acggcgcacg
    17341 ggtcggcgtc ctgcaggaag accgtacgcg ccccgtcgcc gaggaagagc gacacctggt
    17401 acgcctcggg gggcacgagc ccctcgcgca cggcgtgcac gccgcggcgg cgcagcacct
    17461 ccgcggcctc cgccatggcg tcgacgtcgg cgagcacctc ctccaccggc cgcagggacg
    17521 acttcgcccc cttgtacacg gggcagaacg tgcagcggtt ccaggtgcag ttgcggatca
    17581 cgcgcaccag caggctgtgc gcctcgctgg gcgggcggat ggggccctgc tcgaacgacg
    17641 gggtggtcat cacgcgctca gtcgatgtgc agccggcgca ggagcacgcc caccaggtcc
    17701 atgaacacca tgaccgcgac gccgatgccg aggatcacga gccactccgt gccggagatg
    17761 gccgtggtgt cgaacgccgt gttcatgaac ggcgcgtagg tgaacagcac ctggagcccg
    17821 gcgaccaccg cgacgccgag gaggatgatg cggttgccga acgggttgta ggtgaagagc
    17881 gaccggcgca gcgagcggca ggcgaacagg tagaagatct gcaccgacat gaagacgttg
    17941 acggcggagg tgcgcgcggc ctcttcgccc aggtcgttgg cgagcgccca ctcgaacacg
    18001 gcgaaggcgg acacgagcag gatgagtccg gagacgacga tgcggatgag cacgccgcgc
    18061 gagacgatcg gcgccccggg cgcccgcggg tgccggtcca tgatgcccgg ttccttgggc
    18121 tcgaacgcga ggccgaggcc cagcaggacg gcggtgatca tgttgatcca gaggatctgc
    18181 agcggctcga tgggcagggc cgtgcccagc agaacgccgg cgaggatcac cagcccctgg
    18241 ccgacgttgg tgggcaacgc gtaggcgatg aacttgacga ggttgtcgaa gacgccgcgg
    18301 ccctcctcga cggcggcctc gatggaggcg aagtcgtcgt cggtgaggac catgtccgcg
    18361 gcgtccttgg ccacgtccgt gccggtgatg cccatggcca cgccgatgtc ggcctgcttg
    18421 agcgccgggg cgtcgttgac gccgtcgccg gtcatggcca cgaccttgcc cttggactgc
    18481 agagcctcca cgagacggag cttctgttcc ggcgtgaccc tggcgaagac gttcgtcctg
    18541 tcggcgagct cgatgaactc gtcgtcgtgc accgcggcca tctcagagcc cgtgagggcg
    18601 ccgtcggcgg ctccgacgat gccgatctcg tgggcgatcg ccgccgcggt ggcggcgtgg
    18661 tcgccggtga tcatcttcac cgagataccg gcgtggtgac aggcctcgac ggcggcgatg
    18721 gcggccggcc gcggaggatc catcatcgcc tgcaggccga ggaaggtgag gccgccggcg
    18781 acgtcgtcgt ggccgatctc gtcggtcccg tccggcggcg tgccgcgggc gaaggccagg
    18841 acgcgcaggc ccccggcggc cagcgcgtcc acctgcgcgt ggacggccgc ggcgtccagc
    18901 ggcgccggcc tgccggcccc gtccaccgcg gccgagcagc gctcgagcac cctctcgacg
    18961 gcgcccttca cgtagacgac gcggcggcgc tcgcccgcgt cgtgcagcgt cgccatgtac
    19021 tggtaggcgc tctcgaaggg gatcgcgtcc aggcgcggca tcgcggtcgc ctcgccggcg
    19081 gcgaccaggc cggctttggc ggcgctcgtc agcagggccg cctcggtcgg gtcgccggcc
    19141 acctcgtagc ggccgtccgt caggttgagc gcggtgtcgt tgcagaggag gccggccagc
    19201 aggacctcgc gcgcggccgc ggacgggccg gggacgatat ctccctccgg cccgtacccc
    19261 actccgctca cgcgggactc ctcgccggcg gcgacgatgc gctgcaccgt catctcgttc
    19321 tgcgtcagcg tgcccgtctt gtcggtgcag atgaccatgg tgctgccgag cgtctccacc
    19381 gccggcagct tgcggatgat ggcgcgccgc ttcgccatgc ggttcacgcc gatcgccagt
    19441 gtgaccgtga cggccgcggg caggccctcg gggatcgccg ccacggcaag ggcgatggag
    19501 gcgcggaaca tctcgacgag gtcctcgccg cggacgaggc cgatggcgaa ggtgagcgcc
    19561 gcgaggacga cgatgacgac cagaagcacc ttggagaact ggtcgatctt gcgcgtgagc
    19621 ggcgtcttga ggtcgtgcgc cgactggatg agctggctga tcctgccgac ctcggtgcgg
    19681 tcgccggtgc cgacgacgag cccggcgccg gtgccgtacg tgacgagggt ggaggcgtag
    19741 gccatgtcga cccgctcggc gagcaccgtg tcgcgtggca ccacggagtg gtctttctcc
    19801 accggcaccg actccccggt gagcgccgac tcgtccacgc gcaggtcgcg cgtgcgcagg
    19861 agacgcaagt cggcgggcac cttgtcgccg gcctgcacga gcaccaggtc gccgggcacg
    19921 atctcggtgg ccggcaggcg cagttgctcc ccggcccgca gcagcgtcgc ctcggcggtc
    19981 atcgcccggc tgagcgcgtc gatcgcggcg acggccttgg cctcctgcag gtacccgacg
    20041 atggcgttga ggagcacgac gccgaagatg acgatggcgt ccacccactc gtccaggaag
    20101 atcgtgacga tcgccgagac gagcaggatg aggacgagcg ggtcggtgaa ctgcagcgcg
    20161 aagcgccgca gcggaccggg gcctttgggc ggcgtgagct ggttcaggcc gaactgggcg
    20221 cggcggtgct cgaccgcgaa gcggtcgagg ccctccgcgg cgtccgtctc gaggaggtcg
    20281 acgatctcgc cgtcgggaag cgcgtgccag tgttggtcgt cgagcaactg catgggaacc
    20341 cctccgaggc gtcggcggcc ggtgcccgca gatgataccc gagcaccggc cgcttctgct
    20401 cacttaccgg gcgcgcgccg ttgcgccccg ccccgctcac tcgtaatgga tcgcctgcag
    20461 gatgtcgatc ctcgccgccc tgcgcgccgg caggatggcg gcgatcacgc cgacgacgac
    20521 cgccaggacg aggaacacgg ccagctggac gccgggcacc gagaacgtga tcccctgccc
    20581 ggcgaaccgc gtcgtgacca cccacgcgaa cacgatgccg acgacgatgc ccatgatcgc
    20641 gccgatgatc gaggtgatga cgctctcgta ccgcaccgtc gcgcgcacct gcccgcgcga
    20701 catgccgatg gcgcgcacga ggccgatctc gcgcgtgcgc tcgtacaccg ccagcaccag
    20761 cgtgttcacg atcccgaaca gcgagatgat gacgctgagc gcgaggagcc cgtagatgag
    20821 gaggagcagc tggttcacga ggtcgacggt gccctccttg tactgctcgg tggtctgcac
    20881 ctccgccgtg ggcgccgaag ccagcgcggt ctcgatctcc ttctgcgtgg cggcgatgtc
    20941 ggcgcccggc tgcgccttgg cgaccaccat gaacgtctgc ggcttgggga acagcgcgtt
    21001 gtagccggag gtgccgaccg cgatgccgtt gagcatcatg ggatcgcggt agacgccgag
    21061 caccctgagc gtcgcctgct cgccctccac cgtcgtgacc tcgatcgtcc cgccccgctc
    21121 cacgcccagg gacgacgccg tctgttcctc gacgatcgcc ccgtcgagcc gcagctcggt
    21181 cagcacgtcg tcgccgccgc tgatccagtc gaagctccag acgcgctcga tggcgaccgg
    21241 gtcgatgccc cacaccacgg gcagcgaccc gttctcggcc tgcacctgct gggcgtcgag
    21301 cgagaccgcc gtctccacgc cctgcaccga ctggaccctg gtcgcggtgt cggtggggat
    21361 ggtcatgaag ttactgctcg cgatgacgaa gtcggcgcgc accgtgcggt cgtagctgtc
    21421 gatgaacgag ctcttgaggc cctgcgcgag cacggcgacg aagaccacga cggcgaggcc
    21481 gatcatgagc gccgcggcgg tgagcgccgt gcggctcggg ttgcggcggc tgttgtcgcg
    21541 ggcgaggcgg ccgctgatgg gcgccagctt ctgcagcggc cacccgagcc aggccgcgag
    21601 cggcccgacg aagtagcggc tggcgatcgc cacggcgagg aagacgagca cggcgccgaa
    21661 ggcgatggtg ccgagacgga ccacggtgcc gcccggcccg tacatgccgc cgccgatgag
    21721 gaggccgccg aggatggcga ccacgacggc gagcgccggc atgaagcgcg cgaagcgcga
    21781 cggcggcagg acggcgccct cctgcagggc ggccatcggc gggacgcgcg tcgcccgttg
    21841 cgccgggatc accgccgaga gcaccgtgac gacgatgccc acgacgaggg cgatgacgac
    21901 ggtgcgcggc tgcatgacga gcccgctgcg cgggatgtcg aagccggcgg cctggaagac
    21961 gtagttgacg ccggcggcga ccccgagccc ggcgaagagc cccagcaccg aggcgaggac
    22021 gcccatgacg aacgcctcgc cggtgatgct ccagagcacc tgcgcgcgcg aggcgccgag
    22081 cgcgcgcagc atcgcgaact cccggcggcg ctgggcgacc gtcatggaga aggcgttgaa
    22141 gatgatgaag gcgccgacca gcacggcgat gccgccgaag ctgagcagca ccgggcgcag
    22201 gaaggcgccg atcgcgtcgc tgagcgcctt ggtctggtcg gccgccgcct gggcgccggt
    22261 cttgacctcg gtgtactcgg ggagcgcggc acggatgcgg cgcgcgagct cgtccgccga
    22321 gacccccgcc tccgccttga cgtcgatctc ggagacgcgg ccctgcatgt cgaaccactg
    22381 ctgcgcgtcc gagagcgtcg tgtggatgag gatcgagccg cccagcgacg actgggcggc
    22441 gaacctgaac acgccggaca ccgtcgcctc gtgcccgccg ccgtcggtga tcaccgtgat
    22501 cggcgatccg ggcgagagct tcttgtcctg ggcgagcttc tcgatgacgg cgacctcgcc
    22561 gggctgctcc ggcgggcgcc cctccgtgta ctgcgggggc gcgatgtcgt tgggcgagta
    22621 ggagaagaag agcgtcggcg agcctccggt ctccaccacc tcgccgtcca cggccacggc
    22681 gcccatcccg ctcacgtagc cgaacacctc gccgaccccg tccacagcct ccacctgtcg
    22741 caccatgtct tccgggagcc cctcggtggc gccggtgagc tggccggaga acctggcctg
    22801 ccgcgtcacc gtgacgtcgg tccccttgta ggcgtcggtg aagacctgct tgaacccgcg
    22861 gtcgatctgg tcggtgagca cgtacgtgcc gctgatcatc gccacgccga gcaggatggc
    22921 gagcgtggtc aggaacgtcc tgagcttgtg cgccgtgagg ctgcgccacg cgatgcggac
    22981 catcaggcgt cctcgagcga cttgatgacg tcgtagatct cgtcgcggcc gagccgcccg
    23041 cagtcgtggg cgatggcgcc gtccttgagg aacacgatgc ggtccgcgta ggtcgccgcg
    23101 tgcgcgtcat gggtgaccat gacgatggtc tgtcccatga cgtcgcgcga gtgccggagg
    23161 agctgcagca cctcctcccc ggtgcgcgag tcgaggttgc cggtgggctc gtcggcgaag
    23221 agcaccgacg gccgcgaggc cagcgcgcgg gcgacggcca cgcgctgctg ctggccgccg
    23281 ctgagctcgg cgggcttgtg cgtcagccgg tcgccgagcc ccaccgtctc gacgagctcg
    23341 tccacccagg cctggtcacc cttgcgtccg gcgatggtca gcggcaggga gatgttctcc
    23401 gccgcgttca gcgtcgggag caggttgaac gtctggaaga tgaagccgat cttgtcgcgc
    23461 cgaagctgcg tcagggcgcg ctccttgagc gtggtgatct cctgcccgtc gatccagacg
    23521 ttgccggcgg tcgggcggtc cagcccggcg aggatgtgca tgagcgtgct cttgccggaa
    23581 cccgacggcc ccatgatcgc cgtgtacgcg ccacgctgga ggtctatctc cacgcccgcg
    23641 agcgcgcgca cgagcgcgtc gccctggccg taatcccgct cgatgccacg cgcctggacg
    23701 acggcgtcgg cgaggcccac ggtcgtcccg gcctcacagg tggccgccgc cggaccatct
    23761 gcctgcacga tcgtcgtgtc gacgttgtcg gtcacgtctt ctcccttggt cgtcgccgcc
    23821 gctggcggtg cgtggtgggc cgcgcctccc ggcgcggccc accacctgct gtcatctgat
    23881 atccccacgg gacccaccga acggaacccg caccgttcgc gaaggcctac gactgcgccg
    23941 gtccggtccc cacgtccccc tgcacgtcgt tctggggcgt gccggatccg ggcgtctcgg
    24001 gcgtcggcga cgccggcgcg gcaggttcga ccggggcggc gggctcggcc ggggcagccg
    24061 gcgcctccgc gatcccctca ccagccgaaa ccgcgggcgg cacatcggcg acgaccgtct
    24121 ccggcgcggg gaccgccggc gcaacggcag tcacgtccgg cgccgccggc ggagtctccg
    24181 gcgccgtgta caccagcgga gtgatcgcgg cggcctgcgc ggggagcgcc gctgccgacg
    24241 ccgggtacgc ggcgccttgc gccgccgcgg cggcggcgtt cgcggccgcg agagccgcgg
    24301 cctcctcgcg cttgccacga cgccactccg ccacgcccag ggcagccgcg ccggcgccga
    24361 agaccatcat caccgtcacc agcaggggac cggcgaaggg gatccgggac acgacggtcg
    24421 tccccacgac cccgagcgtc acggcgagca tcaggttctc cctgccgcca aagccggtga
    24481 gcaccttctg cgcgaggaac gacgccacac cggtggtgac gaagaagtac gccagaagca
    24541 ccaccagggc gtagggcacc accagcagca gaccgatgag gctgatcacc agcacgacga
    24601 ggatcgccgg gacgacgacg aagaagagca gcgcgcccca cccgagcgac gcccagggct
    24661 tgcgtccgag acgcaggtgc acgccacgca gttgctgcgg catcagcgcc gctgccacca
    24721 gcgccagcac gaggaagaac gcggtctgca cgagccagcc ggccaggctg aagccccaga
    24781 ggcgattctc cagccacgcc cgggccgccc acgcgccggc tccgttccag ctgataccct
    24841 cgatcttctc cgtccgtccg gtgacctgcg cgccgggagc cttgaccagt tccccgccca
    24901 tcaggacgag cgccgagtcc gtcggcgcga ggtcactccc gacgatcgcc ttcgggccca
    24961 gcttgacgtc gccgccgaac gcgacgaccg acttctcgac ggtcccgttg atgacgacgt
    25021 agccgccgaa cgcgaccacg gtgtcggtca cggtgccgtt caccgtgacg tcgccgccga
    25081 acgcgaccac cgtcggcacc tgcgaaccct tggacacgac gatgtcctcg ccgaagcgga
    25141 ccacgtcctc acgcgtcacc acgacgacac cgggctcggt cggggccggc gacgcggcgg
    25201 ccgtggcggc gcccgcaggg ccggcgatcg ccaggcccag cagcaccagg gcggcggcga
    25261 gaacggcgag tgcgagcgat gggcgcagca ctctcaggag tcggtcttct ctcatggggt
    25321 cacctccgcg agcagacatg gtcagctcgc cgtctcgtcg tcggtgggcg gggccggcgc
    25381 cgatgccgcc ggagtgggcg ccggcacggg gggcgccggc gcgggaggcg gcggcggcgc
    25441 cggcacgagc ggtgccatcg ccggcgcctg ggccgggccg cggaacatca cctcggccca
    25501 tcgggcgcac acccagctcc acgcgttgag cacgtgcagc gccacgaaga acacgaggac
    25561 gccaagcggc aggcagagga tgccgaacca cagcggcggc acccaggtgt tgttgatcac
    25621 cggcacgtcg aagacggcga agaacggcat cgacacgaac cagaacacca tcgaggcgcc
    25681 gacgaccagg agcgtcgtcg agacgatccc gaagggcagc ttggcgagca gatagacgag
    25741 gtccttccac gtcgcggcgc agccgaagtg cgccttgagc ttggcccaga cgccgtcgac
    25801 cttctcccac tcccgtgggg cgggcggcac gtcggccccg aggaggtgct tcgcctgcag
    25861 gcgctcgaag gcgccgaaca accaccaggc gccggcgacc acgagcagga tcgggatgcc
    25921 gacccacacc accaccaggc cgatgccgag cgaaaggccc gtgacgagga acacgaagta
    25981 gaacaggccg agcgggaagg cgagccagtt gaagaggatg cccagccagg tccgtgggct
    26041 ccggacgacg ccgaagaagc gtacgagcgc cgagtcgttc aaggggtcac ctcccgtggt
    26101 cttccgcagc ttgcaggaga agcgtagccg cgagaccgcc cggggtcgtc accccggggc
    26161 gggaacctcg gcggaccggc ggtgggattc ggaagccggc cggcataccc ccacgggggt
    26221 acgcggccgg ccggccggct cactcagccg ggctctcccc gggccgcacg agcccgacct
    26281 cgtaggcgag gatcacggcc tgggtacggt cgtgcagccc catcttggcg aagatccgct
    26341 tcacgtgcgt cttcaccgtg ccttcgctga ggaacaggtg ggcggcgatg tccccgttgg
    26401 tcatgccccg cgccaccagc acgaggacct cgcgctcgcg gtcggtgagg tcgtcgagct
    26461 cgctggcatc ccgctgcggg gtcgcttcgg ggcggttgcg gaaggtctcg atgaggcgct
    26521 gcgtcacgga tggggccagc agcgcctccc cctccgcgac cacgcggatc ccggcgacga
    26581 ggtcctcggc gggggtgcgc ttgagcagga acccgctcgc ccccgccttg agcgcctcgt
    26641 agacgtactc gtcgaggtcg aaggtcgtga ggatgacgac gcgcgagctc tccgcgcgcc
    26701 ccagcgcgac gatctcgcgc gtcgcctcga tgccgttcat caccggcatc tggacgtcca
    26761 tgagcacgac gtcggggtcc agccgccgcg tgagcgtgac ggcctccgcg ccgtcgttcg
    26821 cctccccgac cacctcgatg tcgtcctccg tctcgaggat gagccgcagg ccggagcgca
    26881 ccagctgctc gtcgtcggca agcacgatct tgacggtcat gtctcctccg ttctcgcgat
    26941 gcggtaccgc gccgcgctca catggtctcc cccgcgccgt ccagaggcag ccgcgcgcgc
    27001 acgccgaacc cgccctcggg ccgcggcccg gcctcgagct cgcccctgaa ggtggccacg
    27061 cgttcgcgca tgccgagcag gccgcgcccg ccgccggcgg tagccggcgt gacgccgcgg
    27121 ccctcgtcca gcacttcgat cgcgagcgcc cgttcctcgt agcgcaccgt gacggtggcg
    27181 cgcgtcttcc cggcgtgctt cagcgtgttg gtcagcgact cctggacgat gcggtacgcc
    27241 gtcaggtcga ggctcgccgg cagcggccgc ggcgctcccg tgacggtcag ggcgaccggg
    27301 agccccgcct cgcgcacctg gtcgcacagc gtctccagtt gccccatccc gggggtgacg
    27361 tcgcagcacg cctcttcctc gggcgcacgc aggatcccga gcatgcgctc gatgtcgccg
    27421 agggcctggc ggccggccgt ctcgatcgac tccagcgcct cgcgtgccag ctccggcttc
    27481 ttctcgatga cgcgctgcgc cgcaccggcg tgaaggacga ccacgttgag cgcgtgaccc
    27541 acgctgtcgt gcaactcgcg agccatgcgc gcccgctcct cggtgaccgc ctcgcgggcg
    27601 cgcgcctcgc ggtcggccgc gaagagcgcg gcccgccgct cggcccgctc gatgctgccg
    27661 cggtagacac ggacgatcac cgccaccacc cacacgatgg agagcgaggc ccacgtgctc
    27721 agcgccgact gccaggtcag caccggaagg gcgacgaaga cgatgacgat cccgaccgag
    27781 acgacgaagc ccgcagctac ggcgcgcttc cagtggtcga aggccacggt ggcgaaggag
    27841 gcgacgatca ccgcccagat ggcggcctgc gtgaactcgt agccggccag cgactggacg
    27901 cacagcagca cggtcgcggc cacgagactc gtcagcggcg accaccacag caccgccagc
    27961 gtgagcccgc tcgcgacggt caagccgatg agcgccgcgc cgtcggcgtg gtacccggcc
    28021 ggcacggagc cgcgcgtgag cagctcgacc aggccggcgg ccgtgaacac gagagcgagg
    28081 aagacgctga ccgggatcgg atccagcacg cgcgggcgcc gcggtggcgc gggagcagcg
    28141 cccgccggcg cggcgaggat gtcggctggc cgttccacgc ccgcagtata ggtgagttgg
    28201 tggaggcggc gggaatcgaa cccgcgtcca cggacgccca agcggagtct ctacaggctt
    28261 agccggcgca tttgatctcg ctcgcggccc ccgcgtcggc cgggtgccgt ccgccagccc
    28321 cggtcagtct cgggtgcgct cgctcggagc agtgcgaccg caccccagcc cgcttgatga
    28381 caccgcgagc cccggccgcg ggccgccggg tgcgatgcgt cacctaatta cttaggcggc
    28441 gagtgcgaga gaattattct cggcaagtag tgtgttccgg cagtttaacg aggccaccgg
    28501 acctcggcct gctgctccca cccaggtaca gcccatgtcg agacctgttc gcccccaact
    28561 gggcagcctc agtggctgct gctctctgag tataccagcc ccagagacgg caaaaccgcg
    28621 gcgctgccgc ggttttgcct gcagatctca aagctcgctc agtgtctggt gaacctaccg
    28681 cgcaccagac gtcagcccgt ctacgtgggc taatcctcta cggtctgcac ctcacgccgg
    28741 tcgcggcgcc ggaagtacat gttgcggcag agcgggccct cgcccgtggc gtagcagctc
    28801 tgcacggcga tggagcactg cggcaggggc tggcgcgcca ccttgaccac gccgaaccca
    28861 cccttgccgc gctcgatctc ctggaacatc ttcaggtcga tctcgtgcgg gaacaccttc
    28921 tcgatacagc cgaagaacgt gtcaccgtct tccttgaacg cgtacagaaa gcggcagttc
    28981 atggcgagac acgcggccgg atagacgacc ttctcgcact gcgccgagca ttcctgacat
    29041 tcaagctgtt cttcagacaa gcggatcagc ctcctagaca gcacaacggc cggcgttcgg
    29101 tgccggcccg cgaggaatag tagcacgggc cttccagcct gaacaggtgt cttaacgccc
    29161 gcttaatact tcgccgacag ggataacgcc cggtcttcca cgtcgttgtg cccgctcgca
    29221 caagtcctgc attgacaatg catcacgtcg atagtgaaaa tatgcacgta ctgtcgggcg
    29281 ccgaggggat ggcgccgacc cacgcgcttg ccaacgggag gtggcggaca cgcatggacg
    29341 ccgtaatgct cgcacgcatc cagttcgcgt tgaccatcgg ctatcacttc atcttcgtcc
    29401 ccatcagcat cggcgtgggg atgatggtcg tgctcgccga gcgtcgacac tacaagagcg
    29461 ggatcgccgc cgacagggcc gcctccaact tctggatcaa gctgttcacg gccaccttcg
    29521 ccatcggcgt ggccaccggc atcaccatgg agttcgcgtt cggcaccaac tgggccgagt
    29581 actcgcgctt cgtcggcaac atcttcggcg ctccgctggc cgccgagggc ttgatcgcct
    29641 tcttcctcga gtcgaccttc ctcggcatcc tgctgttcgg ccgcaagcgg gtctccagag
    29701 gcttctacta cgcgtccgcc tggctggtgt ggatcggcgc gctgctctcc gcgctctgga
    29761 tcctcatcgc caactcgtgg atgcagacac cggcgggcta cgagatcaag gacggcaagg
    29821 ccgtgctcac gagcttctgg gccgcggcgc tcaacccgtc catcggcccg cgctacttcc
    29881 acaccgtctc cgcggccctg ctgaccggct gcttcgtggt cgcgggcgtg tgcgcctact
    29941 acatgctcaa gaagcggcac gcgcagttcg cgcggcggac catgctctgg gcggcagccg
    30001 tcggcctcat cctggcgatc tccatgccct tcatcggcca ctggggcgcc ctcgtggtcg
    30061 ccgagtacca gccggtcaag atggccgcct tcgagaacat cggcgacacg cagaagaacg
    30121 ctcccctgta cctcttcggc tgggtcaaca gcgccgggga caccaccggc atcagcatcc
    30181 cgagcgggct cagcctcatg ctgggcctga gccccgacca cgaggtcacg gggctcaaca
    30241 gcgtcgctgc ggccgacagg ccgcccgtcc agctcacgtt ccagagctac cacctcatga
    30301 tcgccctggg cttcctcttc atcctcgtgt tcttcgtcgc catcgtcctg cacagactgg
    30361 gtcgcctcga gcgcacccgc tggttcctct gggtgctcat cggctgcatc cctctctcgc
    30421 tcatcgccat caacatgggc tggaccgccg ccgaggttgg ccggcagccc tgggtcgttc
    30481 agggactgat gcgtaccagc gacgccgtgt cgccgctcgt gtcggcaggc gagatctggg
    30541 cgaccatcgg gctcttcgtc ctgatctaca cggtgctctt catcgcctgg ctgcggatct
    30601 tcctcggcat catcaagaag ggccccgagg acccggccga gatgctcgag gccgagaagg
    30661 cgagcgccga gccgctctcg cccgacaccg cagggcccgc tcccgccggc gcggggaggt
    30721 gacgggatga ccctggccaa catctggttc ttcctggtcg cgctcctgct caccgtgtac
    30781 gtgatcctcg acggcttcga cctgggagcc ggcgtgctct acccgttcct cggcaagacc
    30841 gacgcggaca agagcgtcat ccgcgccgcg atcgggccgg tctgggacgg caacgaggtc
    30901 tggctcatca ccggcgccgg agccatcttc gccgcgttcc cgatggtcta tgccatgacc
    30961 ttcagcggct tctacctggc gatcatgctc gtgctcttcg gcctcatcct gcgagccgtc
    31021 tcgctcgagt tccgtcaccg cgacagctcc tggagcggag tctgggacgc cgccttcttc
    31081 gtcggcagcc tggtgccgtc ggtgctggtg ggcgtcgccc tgggcaacgt gatccgcggc
    31141 gtgccgatgg acgcgcaggg caactacatc ggcaccttct gggagctcct caacccgtac
    31201 tccctcctcg tcggcgtcac cggcctcttc ctgctcgtgc agcacggggc cgcgtggctg
    31261 gcggtcaagg cggagggcga gctgcacgc
//
LOCUS       DASUMX010000241        10964 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1092686, whole genome shotgun sequence.
ACCESSION   DASUMX010000241 DASUMX010000000
VERSION     DASUMX010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 10964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10964
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1092686"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..759)
                     /locus_tag="VFZ86_11445"
     CDS             complement(<1..759)
                     /locus_tag="VFZ86_11445"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF005719.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEX5642934.1"
                     /translation="MGESINGTRKQVAEAIQARDAEFIKTLAQDQIDAGAGLLDVNGG
                     VAGGNEVEDLLWLIEIVMGVTDMQLMVDSANPKALDAGVQAIIDKGGKVPFINSISGE
                     QPRIDAVMPLVEKHKCPVVGLCLSDEGIPPTAEDRFAVAKQIFDLCTGAGLPAEDLWI
                     DPLVMAVSADPSAPGVTMDTLKMVKDRLPSRTTGGLSNVSFGLPNRGLLNRTFVAMCA
                     GLGLDGLIVDVRNKQMMATIKSIEALRGEDNFCGS"
     gene            complement(942..1181)
                     /locus_tag="VFZ86_11450"
     CDS             complement(942..1181)
                     /locus_tag="VFZ86_11450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642935.1"
                     /translation="MAGDVIESTHVLFVTGPARERLYRRFSVLFDGRDDVEVRIDRRV
                     AERRRGIGSALDRERRSGDRRRRRPDWIVPPPEGV"
     gene            complement(1254..2060)
                     /locus_tag="VFZ86_11455"
     CDS             complement(1254..2060)
                     /locus_tag="VFZ86_11455"
                     /inference="COORDINATES: protein motif:HMM:TIGR01856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HEX5642936.1"
                     /translation="MSAPAGLPGDYHVHTTWSDGAGTIEDVARRAAAVGLPEIAIAEH
                     CTPLPGPGETWWLRPEQLGAYVDDVRAVAARHADVRVLLGVEAEYVAGQEVELAELLS
                     AWPFEVVVLGIHEVDGLTFDDPALRQDPRWEDPDALIAAYYRTVRDACAWGRFDILAH
                     VDYIGLWGHRPGPAVQPEIDAALDALAASGAAVELNTDRFSDPAGVMYPSPDILRAAR
                     VRGIPLVIDSDAHEVVHVGRAWGEAVAVARAAGFRETLRLSDRALVPLPD"
     gene            complement(2057..3913)
                     /locus_tag="VFZ86_11460"
     CDS             complement(2057..3913)
                     /locus_tag="VFZ86_11460"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5642937.1"
                     /translation="MDDPRNGWHERHRVVTLTITVVVLLTLVAACVGVVSAAASSSSS
                     PSPDAGKVTLRLGWTESPLNLNPFIGYSNSYEIWLLNYDTLVAVGADGLPSKETGLAE
                     DWTLSADEKEWTFKIRQGVTWQDGEPLTARDVAWTFNTIIENDLSLAVYLEDVEKAVA
                     VDDTTLKVSCSAPKANMLLTQVYIYILPEHIWGELSVDELSNTFRNPVPIVGSGPFQT
                     VEFKKDEYVKLVRNPTYWGRQPTVDEVIFQYYTNADTMVQDLKSGAVDGAQVIPATQF
                     KQLQDEAGIEAIPYPLYNWEYIDVNCYESPDSLGNPVLRDAKFRVAMAWAIDREKCAS
                     LAWSGLADPGYGIFPEKGWPEAADPYYRPEAADTIGFDLEKARTLMDEAGYRDDDGDG
                     VREDEQGEPITLRLWARDISPESQVQGKLITGWLQEIGLDIDYSVVDEGALGDDIWNY
                     KGDVYAPDYDLALWDFMGYIDPGDSAACFTTDQIENYNEMCWSNVEYDELTVAQYREM
                     DTAARMELLKQAQGVMYAEQPMIVIDYPSVLQAVNVERWEGWQPYVGGSVWNNFLDRR
                     SYLELKPRVAETAGDEGGSSVMQWVVVGVVAFAGAAIVVALKRRGDGRAVEE"
     gene            4125..5504
                     /locus_tag="VFZ86_11465"
     CDS             4125..5504
                     /locus_tag="VFZ86_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009769997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX5642938.1"
                     /translation="MAPEAARTTVEAPETRWARTVDGACIAYHELGSGPITVVSIPGW
                     ISHLEIYWEQPRYVRFMRRLSQNMRVLVFDKRGLGMSDRVAGSPDLGVLLDDVRAVMD
                     AAGVERAALLGWGTPGPELAAFFAATHPDRTLCLMLYGPIHYRREADFEWGLAPERWR
                     EDVARCMRDWGTLRGAEEFCAIGFVHPPPDSGFDSWAARIARYSATPASYEAFERMWF
                     DTDVRPVVSSIQAPTAAFYAAGDREDEELAVGQAALIPGAVAVPVSTPSEVIWIHDPD
                     PIVSAIEHFIASVRHEEAELDRVLATVLFTDIVGSTDRACELGDRGWAELLQRHHQTL
                     RALIRRYRGTEVKTTGDGFLATFDGPARAVKCAQGVCEAVRPLGIEVRAGCHTGEIEL
                     LGDDVGGVAVHIGARVGALAGPSEVLVTSTVKDLVAGSGLVFQDRGKHHLKGIPDAWR
                     LYAAGADTS"
     gene            5556..6497
                     /locus_tag="VFZ86_11470"
     CDS             5556..6497
                     /locus_tag="VFZ86_11470"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX5642939.1"
                     /translation="MGEMDPVDLRTLERTPLFQALPRGDRGRVARLATVDTYRNGEVV
                     VRAGDPGSTFIVMLSGDALVEPAGGGERMLVSDEFFGELSLIDGRPRAATVTAAGPLK
                     VARIGREDFRALLADEPELAAGLLPGVAAVARDLLRADAGVLPDHGRVGEWRTGDDTA
                     LVEAVGEELDGRDALGWLLLLRHVGVFESLSENHLHRVARLFTIGRFADGDNVVIAGA
                     PGDSLYIILNGRARVRTPSGHAATLDADACFGELALIDGAPRSATVTAIGDLTTAKLE
                     RSRFNKLLRAEPGIAVGLLDGLVAIVRDMEEANAPVA"
     gene            6646..8076
                     /locus_tag="VFZ86_11475"
     CDS             6646..8076
                     /locus_tag="VFZ86_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX5642940.1"
                     /translation="MTEQNFLSQMEPRGSIRLPSTGVPKTRWARTVDGVWIAYHDIGR
                     GPQTLIFTNSMYSHLEVYWELPQFVRFMNRLSASVRVLNFDRRGTGMSDRVELSPSLE
                     ARMDDVIAVMDAAGVERAAVYGWSSGSAALAALHAGTHPERTTALLIDGWLAVKWAPD
                     FPWGVTPEEWDAYLARLVEIWGDDDHGLEIGQLTCGNRPEDGPWDDPDFVRWHSRLAR
                     YSATPGSFEAFERDEYEMDVRDIARHIRVPTAVMVKAGNMPADIRNAEYNAALIPGAR
                     LIRVRGAANIPMFDAIDTYTDAMIGFLRSVEDEERALDRVLATVLFTDIVGSTDKACQ
                     LGDNRWTELLGRHNEVARALVARYRGSEIATAGDGLLATFDGPARAVRCAQGICEAVR
                     PLGLEVRAGCHTGEIELLGADVGGIAVHIGARIAAFAGPSEVLVSSTVKDLVAGSGLV
                     FEDRGEHTLKGVPERWRLYAAVPSGD"
     gene            complement(8187..9821)
                     /gene="groL"
                     /locus_tag="VFZ86_11480"
     CDS             complement(8187..9821)
                     /gene="groL"
                     /locus_tag="VFZ86_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223986.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEX5642941.1"
                     /translation="MAKELKYHDEARRSLERGVNALADAVKITLGPKGRYVVLDKKFG
                     APTITNDGVTIAREIELEDVFENQGAQLVKEVATKTNDIAGDGTTTATLLAQAIVREG
                     LRMVAAGANPMAIKHGIEEAVNEAVASIKKQSKEISGKEEIARVATISARDRGVGDVI
                     ADAIEKVGKDGVVNVEESNTFGLDLEFTEGMQFDKGYLSPYMVTDPDRMEAVLEDPYI
                     LIANQKIGAVQDLLPILEKVIQAGKQLLIVSEDVEGEALATLVVNKLRGTFTSVAVKA
                     PGFGDRRKRMLEDIAILTGGEVITEEMGLKLDNTQISQLGRARKVVVTKDNTTIVDGA
                     GDTEAIKGRIKQIKSEIENTDSDFDREKLQERLAKLAGGVAVIKVGAATEVEMKETKH
                     RVEDALNATRAALEEGIVPGGGVVLVNAIPAIKVDKKSDDVDFRSGKQIIARALEEPL
                     RQIAENAGLEGSVVVNKVKTLKAGEGLNADTDKYEDLVKAGIIDPAMVTRSALQNAAS
                     IAKNILTTECIVADKPEEGGGGMGGGMPGGGMPGMM"
     gene            complement(9841..10137)
                     /gene="groES"
                     /locus_tag="VFZ86_11485"
     CDS             complement(9841..10137)
                     /gene="groES"
                     /locus_tag="VFZ86_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009936171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEX5642942.1"
                     /translation="MKLKPLEDRVIIKAVKEEEKTASGIVLPDTAKEKPMMGEVVAAG
                     DGKWDEDGKKRVPMDVKKGDKVIYGKYSGTEYKTADGDELLILRASEILAVVEK"
     gene            10458..10895
                     /gene="dtd"
                     /locus_tag="VFZ86_11490"
     CDS             10458..10895
                     /gene="dtd"
                     /locus_tag="VFZ86_11490"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015234307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HEX5642943.1"
                     /translation="MRVLLQRVSRAAVAVDGAETGAVGRGFLALVGVTHEDDAAAARR
                     LAAKTARLRVFADDAGLMNLALGDVGGAVLAVSQFTLYAGTRRGNRPSFTGAAPPDRG
                     EAVYEAYVEALRAEGVPVETGMFGAHMQVELVNDGPVTIRLEA"
BASE COUNT         1623 a         3722 c         3774 g         1845 t
ORIGIN      
        1 cgagccgcag aaattgtcct cgccgcggag ggcctcgatg gacttgatgg tggccatcat
       61 ctgcttgttg cgcacgtcga cgatcaggcc gtcgaggccg aggcccgcgc acatggccac
      121 gaaggtgcgg ttgagcaggc cgcggttcgg gagaccgaag ctcacgttgc tgaggccgcc
      181 ggtggtgcgg gacggcaggc ggtccttgac catcttgagc gtgtccatgg tgacgccggg
      241 agccgacgga tcggcggaga cggccatgac cagcgggtcg atccagagat cctcggccgg
      301 caggccggcg ccggtgcaca ggtcgaagat ctgcttggcg acggcgaagc ggtcctcggc
      361 ggtcggcggg ataccctcgt cgctgaggca gagcccgacg acggggcact tgtgcttctc
      421 gacgagcggc atgacggcgt cgatgcgcgg ctgctcaccg gagatcgagt tgatgaacgg
      481 caccttgccg cccttgtcga tgatcgcctg cacgcccgcg tcgagcgcct tgggattggc
      541 ggagtcgacc atgagctgca tgtcggtcac gcccatgacg atctcgatga gccacagcag
      601 gtcctcgacc tcgttgcctc cggcgacgcc gccgttgacg tcgagaaggc cggcgccggc
      661 gtcgatctga tcctgcgcca gtgtcttgat gaactccgcg tcacgcgcct ggatcgcctc
      721 ggcgacctgc ttgcgcgtcc cgttgatcga ttcacccatc aacagcattg aacaacctcc
      781 acgacgatta cccgggacgg acgtctgcag agtatatagg gccgtgcgcc cgcggcgcac
      841 cgccggtcgg attgcctgcg ccagcgcgct gcgaaggccc gccgaggacg ccggcgcggg
      901 catggtgatc aggcgcccgc cggggcgggg tcacgggatg gtcagacgcc ttcgggcggg
      961 gggacgatcc agtctggtcg ccggcggcgg cggtcgccgg accgccgctc gcggtcgagc
     1021 gcgctgccga tgccgcgccg ccgctcggcg acacgacggt cgatcctgac ctccacgtcg
     1081 tcacgcccgt cgaagagcac gctgaagcgc cggtacagcc gctcccttgc cgggccggtg
     1141 acgaacagta cgtgcgtgga ctcgatgacg tcgcctgcca tacgcccagt gtacccctcg
     1201 cggcggcgtc gccaacagca cccgtgcggc cggggtcgtc cgcgaccgtg gatctagtcg
     1261 ggaaggggga cgagggcgcg gtcggagagg cgcagagtct cccggaaccc cgccgcgcgc
     1321 gcgacggcga cggcctcgcc ccacgcccgg ccgacgtgga ccacctcgtg ggcgtccgag
     1381 tcgatgacga gagggatgcc gcgcacacgc gcggcgcgca ggatgtccgg cgacggatac
     1441 atgacgccgg cagggtcgga gaaccggtcc gtgttgagct cgacggcggc gccggacgcc
     1501 gcgagagcgt cgagcgccgc gtcgatctcc ggctggaccg cgggacccgg ccggtgcccc
     1561 cagagcccga tgtagtccac gtgggcgagg atgtcgaagc ggccccacgc gcaggcgtcg
     1621 cgcacggtgc ggtagtaggc ggcgatcagc gcgtcgggat cctcccagcg cgggtcctgg
     1681 cgcagcgccg gatcgtcgaa ggtgagcccg tcgacctcgt ggatgccgag cacgacgacc
     1741 tcgaacggcc acgccgagag caactccgcg agctcgacct cctgcccggc gacgtactcc
     1801 gcctccacgc cgagcagcac cctgacgtcg gcgtgcctgg cggccacggc gcgtacgtca
     1861 tccacgtacg caccgagttg ctccggccgg agccaccagg tctcgccggg gcccggcaac
     1921 ggagtgcagt gctcggcgat cgcgatctcc ggcagaccga ccgcggcggc gcggcgagcg
     1981 acgtcctcga tcgtgccggc gccgtccgac cacgtcgtgt ggacgtggta gtcgccgggc
     2041 agtccggcgg gtgcgctcac tcttcgaccg cgcgcccgtc gccgcgccgc ttgagcgcca
     2101 cgacgatggc ggcgccggcg aacgcgacga cgccgacgac gacccactgc atgaccgagg
     2161 acccgccctc gtcacccgcc gtctccgcga ccctgggctt gagctcgagg tagctccgcc
     2221 ggtcgaggaa gttgttccac acgctgccgc cgacgtacgg ctgccagccc tcccacctct
     2281 cgacgttcac ggcctgcagc accgacgggt agtcgatgac gatcatcggc tgctcggcgt
     2341 acatcacgcc ctgcgcctgc ttgagcagct ccatgcgcgc cgccgtgtcc atctcccggt
     2401 actgcgccac ggtgagctcg tcgtactcca cgttcgacca gcacatctcg ttgtagttct
     2461 cgatctggtc ggtggtgaag caagcggcgg agtcgcccgg gtcgatgtag cccatgaagt
     2521 cccagagcgc gaggtcgtag tcgggtgcgt agacgtcacc cttgtagttc cagatgtcgt
     2581 cgccgagcgc gccctcgtcc accaccgagt agtcgatgtc gaggccgatc tcctggagcc
     2641 agccggtgat gagcttgccc tgcacctggc tctccggcga gatgtcgcgc gcccacaggc
     2701 gcagcgtgat gggctcgccc tgctcgtcct cacggacgcc gtcgccgtcg tcatcccggt
     2761 agccggcctc gtccatgagc gtcctggcct tctcgaggtc gaagccgatg gtgtccgccg
     2821 cctccggccg gtagtagggg tccgccgcct cgggccagcc cttctcgggg aagatgccgt
     2881 accccgggtc ggcgaggccg ctccaggcga ggctcgcgca cttctcgcgg tcgatggccc
     2941 acgccatggc tacgcggaac ttcgcgtcgc gcagcaccgg gttgcccagc gagtccgggc
     3001 tctcatagca gttgacgtcg atgtactccc agttgtacag ggggtacggg atcgcctcga
     3061 taccggcctc gtcctgcagc tgcttgaact gcgtggcggg gatgacctgg gcgccgtcga
     3121 cggcgccgct cttgaggtcc tgcaccatcg tgtccgcgtt ggtgtagtac tggaagatga
     3181 cttcgtccac ggttggctgc ctgccccagt acgtggggtt gcggaccagc ttgacgtact
     3241 cgtccttctt gaactccacc gtctggaacg ggcccgaccc gacgatgggc acgggattgc
     3301 ggaaggtgtt gctcagctcg tccacgctga gctcgcccca gatgtgctcc ggcaggatgt
     3361 agatgtagac ctgcgtcagc agcatgttgg ccttgggcgc gctgcaggag accttgagcg
     3421 tcgtgtcgtc gaccgccacc gccttctcca cgtcctcgag gtagacagcg aggctcaggt
     3481 cgttctcgat gatggtgttg aaggtccagg cgacgtcgcg cgccgtgagc ggctcgccgt
     3541 cctgccacgt gacgccctgg cggatcttga acgtccactc cttctcgtcc gcggagagcg
     3601 tccagtcctc ggccagaccg gtctccttgg acggcaggcc gtccgcgccg acggcgacca
     3661 gggtgtcgta gttgaggagc cagatctcgt aagagttgct gtagccgatg aacgggttga
     3721 ggttgagtgg gctctcggtc cagccgaggc gcaaggtgac cttcccggcg tccggtgacg
     3781 gcgaggacga ggatgaggcg gccgccgaga cgacgcccac acacgcagcc acgagtgtga
     3841 gcaggacgac gaccgtgatg gtgagcgtga cgacgcggtg tcgttcgtgc caaccgttgc
     3901 gtggatcgtc catttgactc cccctgtgct gcacggtccg gagccgcctc gttctctggg
     3961 ggaggcgctc cgagtgttcg cggacgctac cgagcgtacg aggtcggcgt caagaagctg
     4021 cctcgttcgt cgcgcgcggt gaacgccccg gaccttgtcc cacccgacga ccctcactat
     4081 tgtggtaagg caggtcagca cgggctccgt cgaaggactg tgcgatggca ccggaagcag
     4141 cgaggaccac tgtggaagcc cccgagaccc gctgggccag gacggtcgac ggcgcctgca
     4201 tcgcgtatca cgaactgggg tcgggcccca tcaccgtggt ctcgatcccc ggctggatct
     4261 cccacctcga gatctactgg gagcagccgc gatacgtgcg gttcatgcga cggctctccc
     4321 agaacatgcg cgtgctcgtc ttcgacaagc gcggcctcgg catgtccgac cgggtcgccg
     4381 gatcgccgga tctcggtgtg ctgctcgacg acgtgcgcgc cgtgatggat gctgcaggag
     4441 tcgaacgcgc ggcgctgctc ggctggggca cgccgggccc cgaactcgcc gcattcttcg
     4501 ccgccacaca cccggatcgg acactctgcc tgatgctcta cgggcccatc cactatcgca
     4561 gagaggcgga cttcgagtgg ggtctggcac cggagcgctg gagggaggac gttgctcgat
     4621 gcatgcggga ctggggaacg ctgagaggcg cagaggagtt ctgtgcgatc ggattcgtcc
     4681 accccccacc ggactctggt ttcgactcat gggcggcgcg catcgcgcgc tactcggcca
     4741 ccccggcgtc ttacgaagcg ttcgaacgca tgtggttcga caccgacgtg aggcccgtcg
     4801 tctcgtccat ccaggcgccc acggccgcgt tctacgcggc tggcgacagg gaggacgagg
     4861 agctggccgt tggccaggcc gccctcatcc ccggagctgt cgcggtgccg gtcagcacgc
     4921 cttcggaggt catctggata cacgacccgg acccgatcgt gtcagcgatc gaacacttca
     4981 tcgcctccgt acggcatgag gaggccgagc tggaccgcgt cctggccacc gtcctcttca
     5041 ccgacatcgt cggttcgacc gacagagcgt gcgagctcgg cgaccgcggc tgggccgagc
     5101 tcctccagcg ccaccaccag accctgcgcg ccctcatccg gcggtaccgg ggcacagagg
     5161 tgaagacgac cggcgacggc ttcctcgcca ccttcgacgg ccccgcacgc gcagtgaagt
     5221 gcgcgcaggg cgtgtgcgag gcggtccggc cgctcggcat cgaggtgcgg gccgggtgtc
     5281 atacgggaga gatcgagctg ctcggcgacg acgtgggtgg agtcgccgtc cacatcggtg
     5341 cccgtgtcgg cgctcttgca ggaccgtccg aggtcctcgt gacctccacc gtcaaggacc
     5401 tggtggccgg ctccggcctc gtgttccagg accgcggcaa acatcacctc aagggcatcc
     5461 cggatgcgtg gcgactctac gccgccggcg ccgacacgag ctgacctgct acgcgtcgcg
     5521 gccggccgac tgttgcgacc gcagaagggg ggaccatggg cgagatggat ccggtcgacc
     5581 tgaggacgct cgagcgaact ccacttttcc aggcgctgcc tcggggggac cgcgggcgtg
     5641 tggcgcggct cgcgaccgtg gacacctacc ggaacggcga ggtggtcgtc cgcgccggtg
     5701 accccggcag caccttcatc gtcatgctga gcggagacgc cctcgtcgag ccggccggcg
     5761 ggggcgagcg tatgctcgtg tcggatgagt tcttcgggga gctctcgctc atcgatggcc
     5821 ggcctcgggc tgcgaccgtg accgctgccg ggcccctcaa ggtggcccgc atcggccgcg
     5881 aggacttccg tgcgctcctc gccgacgagc cggagctggc ggccgggctg ctccccggcg
     5941 tggccgctgt ggcgcgcgac ctgctgcgcg cggatgccgg cgtactcccc gaccacggcc
     6001 gcgtcggcga gtggcgcacg ggggacgaca cggcgctggt ggaggcggta ggtgaggaac
     6061 tcgacggccg ggacgcactc gggtggctgc tgctcctgcg gcacgtcggc gtgttcgaga
     6121 gcctcagcga gaaccacctg caccgcgtgg cgaggctgtt cacgatcgga cgcttcgccg
     6181 acggagacaa tgtggtcatc gccggtgcgc ctggcgactc gctgtacatc atcctgaacg
     6241 gccgggcgcg cgtaaggaca ccgagcggtc acgccgccac cctcgacgcc gacgcctgtt
     6301 tcggcgaatt ggccctgatc gacggcgcgc caaggtccgc gacggtgaca gccatcggcg
     6361 acctgacgac ggcgaagctc gaacgctcga ggttcaacaa gctgctcagg gccgagccgg
     6421 gcatcgccgt cggcctgctt gacggcctgg tggccatcgt gcgcgacatg gaggaggcga
     6481 acgcgccggt cgcctgaggg ccaagccgtg ccggcgcagg tgcgcgatga acgacctccc
     6541 aagaacacga tgggcgacga ccgcggacgg cgcctcgatt gccaaccagg tcctcggggg
     6601 cggcgagcgg acgatcgtcg tggggcgact gtactcgctc acgccatgac cgagcagaac
     6661 ttcctcagcc agatggaacc ccggggcagc attcgattgc ccagcaccgg cgtccccaag
     6721 acgcgctggg cgcggaccgt cgacggcgtc tggatcgcgt atcacgacat tggccgcggt
     6781 ccccagacgc tcatcttcac gaacagcatg tactctcatc tggaggtgta ctgggaactg
     6841 ccgcagttcg tgcggttcat gaaccgcctc tcagccagtg tccgcgtcct caacttcgat
     6901 cggcgcggga cgggcatgtc cgacagggtg gagctctccc cgtccctcga agcacgcatg
     6961 gacgacgtca tcgccgtcat ggatgcggcc ggcgtcgagc gtgccgcggt ctacgggtgg
     7021 agcagcggct ccgcagcgct cgccgcgctc cacgccggca cccatcccga gcggaccacg
     7081 gctctcctga tcgacggctg gctcgcggtg aagtgggctc ccgacttccc ctggggagtc
     7141 acacccgagg agtgggacgc ctacctcgcc agactggtcg agatttgggg tgacgatgat
     7201 cacgggctcg agatcgggca gctcacctgc ggcaacagac ccgaggacgg cccctgggat
     7261 gaccccgact tcgtgcgctg gcactccaga ctcgcccgct actccgcgac cccagggagc
     7321 ttcgaggcat tcgagcgcga cgagtacgag atggacgtgc gtgacatcgc ccgccacatc
     7381 cgcgtaccga ccgccgtcat ggtgaaggcg ggcaacatgc ccgcagacat ccgcaatgcg
     7441 gagtacaatg cggcgctcat ccccggagcg cgactgatcc gtgtccgagg agccgccaac
     7501 atcccgatgt tcgacgcgat cgacacgtac acggatgcaa tgatcgggtt cctccgatcg
     7561 gtcgaagacg aggagcgcgc gctcgaccgt gtcctcgcga ccgtcctctt cacggacatc
     7621 gtcggctcga cggacaaggc ctgtcagctc ggcgacaatc gctggacgga gctgctcggc
     7681 cggcacaacg aggtcgcacg ggcactggtt gcgcgctaca ggggatccga gatcgccact
     7741 gccggcgacg gcctcctcgc caccttcgac ggcccggcgc gagcggtgcg atgtgcgcaa
     7801 ggcatctgtg aggcggtacg gccgctcggc ctcgaggtcc gcgccggctg tcacaccggc
     7861 gagatcgagc tgctgggcgc ggacgtcggc ggcatcgccg tgcacatcgg cgcccggatt
     7921 gcggcgttcg ccggcccctc cgaggtactc gtcagctcca ccgtcaagga cctcgtcgcc
     7981 ggctctggcc tcgtcttcga ggaccgcggc gagcacaccc tgaagggcgt gccggagcgg
     8041 tggcggctgt acgccgcggt gccctccggc gactgagtgg cctagagaac ggcgaaggcc
     8101 ggcggtgcgt gcgcaccgcc ggccttcgac cggacgcgaa agaggcgtgc tcttccgtat
     8161 ggcttagaag cgagtcgcga ctcggatcac atcatccccg gcattccgcc gccgggcata
     8221 ccgccgccca ttccgccgcc gccctcttcg ggcttgtcgg cgacgatgca ctcggtggtg
     8281 aggatgttct tggcgatcga cgcggcgttc tggagcgccg aacgggtgac catggcgggg
     8341 tcgatgatgc cggccttgac caggtcctcg tacttgtcgg tgtcggcgtt gaggccttcg
     8401 ccggccttga gcgtcttcac cttgttcacg acgaccgaac cctcgaggcc ggcgttctcg
     8461 gcgatctgac gcagcggctc ctcgagcgcg cgagcgatga tctgcttgcc cgaacggaag
     8521 tccacgtcgt ccgacttctt gtcgaccttg atcgcgggga tggcgttgac gagcacgacg
     8581 ccgccgcccg gcacgatgcc ctcttcgaga gcggcgcgcg tggcgttgag agcgtcctcg
     8641 acgcggtgct tggtctcctt catctcgacc tcggtggccg cgccgacttt gatgacggcc
     8701 acgccgccgg ccagcttggc gagacgctcc tggagcttct cacggtcgaa gtcggagtcg
     8761 gtgttctcga tctcgctctt gatctgcttg atgcggccct tgatcgcctc ggtgtcgccg
     8821 gcgccgtcga cgatggtcgt gttgtccttc gtcacgacga ccttacgcgc gcggccgagc
     8881 tgcgagatct gcgtgttgtc gagcttgagg cccatctcct cggtgatgac ctcgccgccg
     8941 gtgaggatgg cgatgtcctc gagcatgcgc ttgcggcggt cgccgaagcc gggggccttg
     9001 accgcgacgc tggtgaacgt gccgcggagc ttgttcacga cgagggtcgc gagggcctca
     9061 ccctcgacgt cctcggagac gatgagcagc tgcttgccgg cctggatgac cttctcgagg
     9121 atcgggagga ggtcctgcac ggcgccgatc ttctggttgg cgatgaggat gtagggatcc
     9181 tcgagcacgg cctccatacg atcggggtcg gtcaccatgt acgggctgag gtagcccttg
     9241 tcgaactgca tgccctcggt gaactcgagg tcgaggccga aggtgttgct ctcctcgacg
     9301 ttgaccacgc cgtccttgcc gaccttctcg atggcgtcgg cgatgacgtc gcccacaccg
     9361 cggtcgcggg cggagatggt ggccacgcgg gcgatctcct ccttgccgga gatctccttg
     9421 ctctgcttct tgatcgacgc gacggcctcg ttgacggcct cttcgatgcc gtgcttgatg
     9481 gccatcgggt tggcgccggc ggccaccatg cgcaggccct cgcgcacgat cgcctgggcg
     9541 aggagcgtag cggtcgtcgt gccgtcaccg gcgatgtcgt tggtcttggt cgcgacttcc
     9601 ttgacgagct gggcgccctg gttctcgaag acgtcctcga gctcgatctc gcgagcgatg
     9661 gtcacaccgt cgttggtgat cgtcggggcg ccgaacttct tgtcgagcac cacgtaccgg
     9721 cccttggggc cgagggtgat cttcacggcg tcggcgagcg cgttcacacc gcgctcgaga
     9781 gagcgccggg cttcgtcgtg atacttgagt tccttagcca agattcgtca gcctcccctc
     9841 ttatttctct actacggcca ggatctcgct ggcgcgcaga atcagcagtt cgtcaccgtc
     9901 ggcggtcttg tactcggtgc ccgagtactt gccgtagatg accttgtcgc ccttcttgac
     9961 atccatgggg acgcgcttct tgccgtcctc gtcccacttg ccgtcgccgg cggcgacgac
    10021 ctcacccatc atcggcttct ctttcgccgt gtcggggagc acgatgccgc tggcggtctt
    10081 ctcctcttcc ttgaccgcct tgatgatgac gcggtcttcg agcggcttga gcttcatgca
    10141 gcaaccctcc ttggtgtgta caccgtggtt tctctcgact tggtactggc actctcgggg
    10201 agagagtgct aatccacaca gctggctatg gtatgaccgg tcctgggaga tgtcaaaccc
    10261 cacagtgcat acgaggcaga tacccaggtg acgccgtagg aaagtgcttg tttgcaggta
    10321 gtatgatgcc ccgcgatgac gacgtcgcgc ctgtcagtcc agacgcacga gtgccgccgg
    10381 gcttcgccgt tgtcgcacct ggcctccgcc ggaccgcctc gctaggattg cggcacgatg
    10441 aacgcacgcg tctccgtatg agagtgctgc tgcagcgggt gagccgcgcc gcggtcgccg
    10501 tggacggcgc ggagaccggc gcggtcggcc gcggcttcct cgccctcgtg ggcgtcaccc
    10561 acgaggacga cgcggccgcg gcccgcaggc tcgccgccaa gacggcgcgg ctgcgcgtgt
    10621 tcgccgacga cgccggcctg atgaacctgg cgctcggcga cgtcggcggc gccgtgctcg
    10681 ccgtctcgca gttcaccctg tacgccggca cgcggagggg caaccggccg agcttcaccg
    10741 gagccgcgcc gccggatcgc ggcgaggccg tctacgaggc ctacgtggag gcgctgcgcg
    10801 ccgaaggcgt gccggtcgag accggcatgt tcggcgcgca catgcaggtc gagctggtca
    10861 acgacggccc cgtgacgatc cgcctggagg cctgaccggg agctaagccc ggagacgacg
    10921 gcgcccgggc cgcctgcagg cggcccgggc gcatgagcac acgg
//
LOCUS       DASUMX010000242        29353 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1236812, whole genome shotgun sequence.
ACCESSION   DASUMX010000242 DASUMX010000000
VERSION     DASUMX010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 29353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29353
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1236812"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..461
                     /locus_tag="VFZ86_11495"
     CDS             168..461
                     /locus_tag="VFZ86_11495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="C2H2-type zinc finger protein"
                     /protein_id="HEX5642944.1"
                     /translation="MPAVTTPKTFVCGVCSATLKSQRDLDNHMCLLHDDCGEAHLGTP
                     ITFRCVTCGEAFALRSDLFSHQKELGHGHPGEWDKARPAGAERPRRRPQSRGR"
     gene            639..1106
                     /locus_tag="VFZ86_11500"
     CDS             639..1106
                     /locus_tag="VFZ86_11500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642945.1"
                     /translation="MRRVIAILALTGAVLLLAAAPAVAAEYRVTKASGALAGRVAPGP
                     DPGQGDRIGIVRDLSKFRGAVYSRQTSSGYTGWPVSNGTSYVAWVTKSSTTRFLVKKV
                     PWGATAGRVTRLSNGRWLAQKRVDGRWRTVGRIQEGCRGQWAAGAARLLLWQR"
     gene            1412..3499
                     /locus_tag="VFZ86_11505"
     CDS             1412..3499
                     /locus_tag="VFZ86_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEX5642946.1"
                     /translation="MVEFTRRQFIKIGGGGLVGLWVGSQFGGFTQVAEAAIPGGSLDP
                     VNVPKFQTPLLIPPVMPKAGTIKLKGGKNADYYEISMKQFAQQILPATLPPTTVWGYG
                     AVAAASKRGLMIHNAPSLTIEAQAGRPVRVKWINGLVGTDGNYLPHLLPVDPTLHWAN
                     PPGGTAERDTRPMFMSTPGPYTGPVPIVTHVHGAVGVGDESDGYAEAWYLPAASNTPA
                     GFADRGTWYDFFAGKAAAGYGATWGPGYATFQYPNDNRASTIWYHDHALGMTRVNVYA
                     GPAGFYIIRGGPAGDGAVLDSRTGLAAILPGPAPKENDTFPSTKAYYEIPIAIQDRAF
                     NADGSLFYPDTRAFFDDDAGPYIPASDISPIWNPEFFGNTIMVNGNTWPFLNVEQRRY
                     RFRLLNGCQSRFLILDFSAITGAEVWQIGNEGGFLSAPVNITGLGNQLLMGLAERADL
                     IVDFTNVPAGNYVLGNVGADAPFGGLPIEGPELADPASTGQIMQFRVGPVLAPDATTP
                     PQFLVLPPLTPLPAPVRSRPLALLEMMSMADPARGPAAAMLGTVDTVNGTAMHHEWMA
                     AVTENPQVGDTEVWEFYNFTADAHPMHVHEVVFEVVDRQPISFTEGAMGPENVALAGP
                     ARPPEAWESGFKDTVTAYPGEVTRIRAQFAKSGQFVWHCHIVEHEDNEMMRPYRIGPE
                     QPGQPMPHTHTMP"
     gene            complement(3655..4323)
                     /locus_tag="VFZ86_11510"
     CDS             complement(3655..4323)
                     /locus_tag="VFZ86_11510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642947.1"
                     /translation="MATREQQDDRLPARPAAPVFASVLGPEDRLPDRPFTDPQETKGD
                     LLVMSRMLELERELALTWGPDIDGPGISEQTSAGGRRLLAVPDGRALLRARDVTAVGF
                     FGRLREDVDHAVLFEHERRIAQLFPRYAPLGFLSYLDVGPEHGRYGNLILFWTPDVPD
                     AWHRGEAHRRAVADAPGHYLHIRLHKGRIPGPFMGAGEVQVLRTQYLDFAAGAPWRAL
                     RIYT"
     gene            complement(4581..6605)
                     /locus_tag="VFZ86_11515"
     CDS             complement(4581..6605)
                     /locus_tag="VFZ86_11515"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX5642948.1"
                     /translation="MKRLVLPILMALLLALVLPVAASALTYDAAVDELVANGYTTDIE
                     DALTDLGTDPQLGFRLAGTWAEHDASYLVRDELEAMGLSDVRLEAVPVDAWDFKGATV
                     EVAGETLTASSFAGVPGTDGPLEAELVYVGTGTAAEFDAAGIVAGKLVLVDGEFDDWW
                     LSFVGAEATHRGAAGVVMTYGENTYPWYSYPEALGSNDGEYDDDWVPMVYVAWRDGDW
                     IKAKLAAAGGSLMATMTSDVKITLAEDGGVGYNVVGVLEGSDPDLAPVLLASHLDAHF
                     RAGLDDTGAAVNELLTAKAMTDSGVQPRRTVIFFFTCAEEYGYTDCWYDWAIGAWYAI
                     TQEHPDWPGKLAMMLNIELMAETGADFRMRAAADVLPFLEAVAADSGDALPYGIEAEP
                     TPSTWTDQWSWNASGVPTVTTSAGGPEYDRYYHTDLENKARVDHDYLGGIAKWNHRVI
                     QGLDEGVLPYDLRARGDQLLGTIQPGKLVRAGVSEAAATRFKTAATKFRAAAWQWELR
                     KAKTPKGKVDAVNEKLVALEKTINATFTSRDQYDFTAYPHEAGTTDAIYLTKAITAAK
                     TRNAARAQQMISWWVGINWYASLFSPEVCAEDLVRHYPDYEHITWGALGTPPLVVDCI
                     DEYVMLGEGDFAGAIAGLRADRAAVRADLRDRIDTMTAVLNDLTKGVNAL"
     gene            complement(6783..8651)
                     /locus_tag="VFZ86_11520"
     CDS             complement(6783..8651)
                     /locus_tag="VFZ86_11520"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013873057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEX5642949.1"
                     /translation="MSALPTDAARPALEAAGELARQLRVDSIRSVASAGSGHPTSSLS
                     AADLMAVLFVRHLHYDWSRPDRPDNDHLIFSKGHASPLLYSLYKAVGVVDDRELLETY
                     RRAGSRLQGHPTPALPWVDVATGSLGQGLPDGVGVALAGKRLDRADFRVWVLCGDSEM
                     AEGSMWEALDKASLYELDNLTVIVDVNRLGQTGPTGLEWDLEAYRRRVEAFGCRALVV
                     DGHDLEQIDDALSLAARSQLPTVVLARTVKGKGVPEVEDAVGWHGKPLKEDVAEAAVR
                     ALGGVSDHRVETPPRPERPAAAVRERRALALPKYEMGMKVATRAAFGDALAALGAWPE
                     VVAIDGEVGNSTFTEKFRDAHPDRFFQMYIAEQQMIGTAVGMSVRGYIPFAATFAAFL
                     TRAYDFIRMAAVSGADIRLCGSHAGVEIGQDGPSQMGLEDLAALRAVHGSTVLYPSDA
                     TSAAALTATMAELGGVSYLRTTRGAYPVLYPSDEEFPVGGSKLLHASADDRVLLAGAG
                     VTLHTCLAAAEQLRERGIPARVIDLYSVKPLDADGLAAAAREAGRRIVVVEDHYPEGG
                     LGEAVLAALAPLREEFRVEHLAVRGLPGSGTPAELMAAAGIASADIEAAALRLLEG"
     gene            8913..9620
                     /locus_tag="VFZ86_11525"
     CDS             8913..9620
                     /locus_tag="VFZ86_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX5642950.1"
                     /translation="MAILYAGTSGYAYPSWKPEFYPADLPAARFLRHYAGRLNCVEIN
                     YTFRRMPSEKALTAWAEATPPGFAFAVKAHQAITHRNRLKEGAREPLEYFLGTLAPLR
                     DAGRLGPVLLQLPPNLKLDLERLERFLELLPRELRFAFEFREASWFREEVYALLRAHG
                     AALCVAEAEELTVPDVVTAGFAYYRMRKPPYDEAALASLRRRAEGLTGGGRDVYVVFK
                     HEEEAGGALEAERLLAG"
     gene            9716..10462
                     /locus_tag="VFZ86_11530"
     CDS             9716..10462
                     /locus_tag="VFZ86_11530"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX5642951.1"
                     /translation="MQYEEFLDLVGSRRTIRAIKPDPVPDDVVRKLLEAARWAPTGFN
                     MQPVELLVVADAGLRASVRSIVDDYKNADFFALEATREEWQGSPWSAETHGPWRCPEA
                     PVMIAVLGDTRRRVGLPMAARYATQKGDSIFESSLANAFLYLLLAAHSLGLAAQPVSG
                     VKYPSVQGRVKHLLNLPDFIYVYDLVLAGWSAMDKPVAGKLTRSLDEMVHYDRATDDE
                     FPSEEEIRRQIVRLRAGNVARHVEGDEAGG"
     gene            10567..12645
                     /locus_tag="VFZ86_11535"
     CDS             10567..12645
                     /locus_tag="VFZ86_11535"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEX5642952.1"
                     /translation="MDPSEFHAPGAGKIVTSPEGYAAFVPAPLPPRISYTKDLVLALS
                     RADAALGELSGVGGELPDPQLLDASFKRQEALCSSRIEGAQVSLSDLYLDQVGAARQT
                     VEREHLREVRACISTLRFGVELLREEPLSLEFVKALHERLLRGEVGGARTPGEFRTSQ
                     NWLGPPGATMATATYVPAPVDQLPEALAAFEQFLQERGRLPDLIQCAMLHAQFESIHP
                     FVGGNGRLGRLLITLFLIERERLTRPLLYLSAFLEGHRDEYYALLQRVRTHNEWTPWL
                     TFFAVGVHETAERATRQARALIRHYERDREKVKGHALRLADELFRTPAMTVPEAKRIL
                     EVTDPTARRAVRELEARGLLAEWSEKRWPRVYLARHVLDAVLHPLEDLRSGPGTADLG
                     TVVKSEATQAQAKPARRADRLMAEAMDTIDAARARGVQLRLTGGLAVRRYCIDLDFMD
                     REYSDIDFVGRSDQKKEIRDVFETLDYTENRYVSQSTDSGQLQYIKNEALEGMKAESK
                     AAAAGERSTYEPPLVDHIDIFLDVMRMDHDIDVGERLEIDDYAISPADAFIAKMQIGK
                     INQKDVHDVIALVKDVPLRDVDDDASIDLLYIAEVCSHDWGLYQDITTNLDIALAMVD
                     DYGLTEAQQDRVYARLAAIKESVESASKTLRWRLRATVGDRVAWRREIEDTEGTQIIA
                     PEWDWRRDLG"
     gene            12661..14640
                     /locus_tag="VFZ86_11540"
     CDS             12661..14640
                     /locus_tag="VFZ86_11540"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX5642953.1"
                     /translation="MTGMVPRDVYELTAVADPRLSPDGTTVAYVAGSVDEKGNAYPAA
                     VWLAAADGSAPPRAFTSGEKADADPRWSPDGTRLAFTSDRADKASQLYVMPVAGGEPR
                     KLTSLKEDVTQAAWSPDGTRIAFVARVRDAAYEEEDDKKRQPRRLTRLQYKLDSVGWT
                     ADRPQHLFVVPADGSAEPEQVTHGDYEDHSPCWSPDGRTLAFVSARHEDWDLEMVNDV
                     YLVDAAGGEPRRLTQGGGSIGSVSWAPDGSRLAVERYAGVYDDPKHTDIALVDPADGA
                     ITPLTASLDLNCSTYPGMREPLWDGDGLVFVAEDHGRVHVYRVPADGSGEPRLIAGGE
                     RVVTGLDVAGSRLVFTASEPARLSELYDGGPAAGGDEDPAAVGRRLTSAGDAFAAGRE
                     LVRPERFTAVSADGSQVDAWIMRPAGYEEGKKYPTLLNIHGGPYGQYGVGFFDEFQVY
                     CGAGYAVVFSNPRGSSGGTEAWARAIRGPGDEGPGWGSVDYEDCMAAVDEAVRRFDFV
                     DADRLGVIGGSYGGYMTSWIVGHTDRFKAAVSERAVNNFDSQWGSSDFGWDFKGYTGT
                     FLYEDLDLYRGVSPVTHAENIHTPLLILHSEEDLRCPVEQAEHLFVTLRLLKRPVELV
                     RFPAESHELTRSGSPLHRVQRFDLVVEWFDRHLKG"
     gene            14653..16725
                     /locus_tag="VFZ86_11545"
     CDS             14653..16725
                     /locus_tag="VFZ86_11545"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEX5642954.1"
                     /translation="MDAAAFGERASGHLVTAEGGHPAFMPAPLPPELTFTAELAERLS
                     AADAALGELSGLAGGRRDPQILVAPFLRQEAVLSSRIEGTPATLVDLLFDEVAAAPDL
                     ELRESLREVRNYVAALQYGVERLADVPLGTTLVLELHERLLRGVRDAAWTPGEFRSGQ
                     NWIGPPGSTLETAVYVPPPVPEMHAALTEWDRFLGERTGLPPLVQCALMHERFEAIHP
                     FLEGNGRLGRLLITLFLIERGRLSHPLLYPSAYIEAHRDEYYDRLQAVRTDGAWEPWL
                     LFFLDAVRETAERASAQVRALMALREQYRWKVSGYARELVDDLFRTPFVTVPEAQQAL
                     GVSNATARKAVRELQEWGMLEELAARRWPRAYIARPILDAIQGPLEDLRLRSEAQSRS
                     APLKSVTDVEEPPEKPAERHMAEAMALIDEARRYGVQVRLMGGLAVRRYCTDLGFMDR
                     EYSDIDLVGLSVQNRELHEVLTRLGYAENRAVTEATGAGQLQYVKTQVLEGAGGAYRI
                     GAGQRDDRPAPLVDHVDLFMDVMRMDHDLDVRDRLLVDDYAISPADAFIGKLQIGRLN
                     AKDAHDVIALLKDVPLRQVDDETSICVPYVAETCAGDWGLCQDVLANIDAVLQMVDDY
                     DLTEEEAARVYGRLAALREAIEAEEKSVGWRLRARVGRRVAWRRTIEDTEGTDVIAPE
                     WDWRRDLG"
     gene            16813..17100
                     /locus_tag="VFZ86_11550"
     CDS             16813..17100
                     /locus_tag="VFZ86_11550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase"
                     /protein_id="HEX5642955.1"
                     /translation="MAYVLIEHRVGDFETFRSVYLDDAARRGRLGSRGGRVFRVADDP
                     GNVIVILEWDTVEHARDFAGSLELEQAMEWSASDVSTPRVTVLDVAIESPR"
     gene            17122..17409
                     /locus_tag="VFZ86_11555"
     CDS             17122..17409
                     /locus_tag="VFZ86_11555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642956.1"
                     /translation="MVYVVLEHEIHDFETLKQVYTDDAERRRRMGCRGGRIWRGADDP
                     NMVSVIMEWDTVENAREFAGSFELEQAMHWATVKAPTTRVTVYQEAHSSAY"
     gene            17541..17861
                     /locus_tag="VFZ86_11560"
     CDS             17541..17861
                     /locus_tag="VFZ86_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018816325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX5642957.1"
                     /translation="MADEYLPDVYLGFREHYPAVAEALDGLGEAVDGAGPLGERDVRL
                     VKLGLAVGALAEGAVKSNARKALAAGASPDEVRQVGILAITTCGFPTAIAGLGWIDEV
                     LKPG"
     gene            complement(17892..18605)
                     /locus_tag="VFZ86_11565"
     CDS             complement(17892..18605)
                     /locus_tag="VFZ86_11565"
                     /inference="COORDINATES: protein motif:HMM:NF014060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide amidohydrolase"
                     /protein_id="HEX5642958.1"
                     /translation="MNDDLQPLPGVTVTAAERAVRISSERPLRVLGSAVVGGGFGVTR
                     EILNVHVDDQYDGERPDEDLAAVAAELGIHGPFVGLMTAAYTQFARCAVERLGDLAVA
                     AVVSVGLSNTSSAGVTPPIGAGPAGGAAAPGPGTINIVLLVDGALTPAAMVNAVITAT
                     EAKTMTLAAWDVKTPEGDAASGTSTDTVVVACTGRGEELGYAGPATPVGWLAARAVRA
                     AMTRICEEKVARDGGRHGW"
     gene            18753..19097
                     /locus_tag="VFZ86_11570"
     CDS             18753..19097
                     /locus_tag="VFZ86_11570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642959.1"
                     /translation="MPLLTGSIEADVPVSFADREWREFIGRSVYDRFPEGYEDLRSSL
                     AELDADDGRVTIAALGTGRARVDVEIDYTPRDPADPGSDVARAQRELDRDLDKYRDFV
                     LRRCDEELCRRN"
     gene            19253..20098
                     /locus_tag="VFZ86_11575"
     CDS             19253..20098
                     /locus_tag="VFZ86_11575"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEX5642960.1"
                     /translation="MSRGGAPAFADRPNLSVLLQEPRYEVIPVQGIEQKVAALPYGAT
                     VTVTASPAQGLERTLDVSTALAARGYRVVPHFAARMVTGRGHLERLVQRLEGTEIREV
                     FVIGGDADPPAGDYADAGDLLEELVGMAHPFARIGIGGYPEGHPLVPDDRLLEALLRK
                     QPFASHIVTQLCFDAGALAAWVADIRAAGVEMPVVVGLPGVVERRKLAEISLRTGVGS
                     SLRYLRKHGRQIVTLARSRRYDPTPLAEAVADHAADEALGIRGVHLFTFNQVEQTRDW
                     VAGLA"
     gene            complement(20112..21017)
                     /locus_tag="VFZ86_11580"
     CDS             complement(20112..21017)
                     /locus_tag="VFZ86_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX5642961.1"
                     /translation="MSRKGWLLFISLCVIWGVPYLFIRIAVRELPPPALVFLRTAPAA
                     LLLLPFALRRGEVRRVLRSWPWVLLYTLVELCIPWLLLAHAEERISSSLAGLLIATVP
                     LIGAVLYRAFGVADHFDARRLTGLLVGFAGVVALVGLDIASSDALGIAETIVVAFCYA
                     VGPIIISRRLADLPAIGVIAASLVVTAVLYAPPGIATMPASLSAETIGSVAVLAVVCT
                     AVAFLLFFALIREVGPARTTVITYVNPLVAVLLGVALLSEPFTLGIAIGMPLILAGSL
                     LATARSLRATGPPPRDELEAGPPVP"
     gene            21176..21685
                     /locus_tag="VFZ86_11585"
     CDS             21176..21685
                     /locus_tag="VFZ86_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX5642962.1"
                     /translation="MDVFEAIEKRYSVRGYKPDAVEDEKLASVLEAARRAPTAANRQP
                     FRMLVVKTQGREEELARVYGRRWLTQAPLVIAFVAVPGEAWRRMDDKPYDEVDATIAM
                     DHLVLAATALGLGTCWIAAFDPDAAREVFGLPDDVELVALTPLGYPDKAPAATGRRPL
                     QELVRYERW"
     gene            21763..22179
                     /locus_tag="VFZ86_11590"
     CDS             21763..22179
                     /locus_tag="VFZ86_11590"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX5642963.1"
                     /translation="MGFVDETIIVAVDVADAYSLWLDYEQYPRFMEGIDEVAVVGYCR
                     LRWRGVVCGETLVWESDVVDRIQDTRLRWRAVDGRETGDVDVQKLDHRQTLVHFQLEY
                     DPTVWGVDPVRLEECLRTRVRNALSAFKELAERAVA"
     gene            22379..23092
                     /gene="ftsE"
                     /locus_tag="VFZ86_11595"
     CDS             22379..23092
                     /gene="ftsE"
                     /locus_tag="VFZ86_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEX5642964.1"
                     /translation="MIVFEDVTKIYDPDVVGLQDFTCHIGKGEFVFLVGASGSGKSTL
                     IKLVLKELVPDSGRILVAGRNLGTLRHSKVPLLRRNIGCVFQDFKLLPNKTVYDNVAY
                     ALEVVGEPRRSIRRKVPEILSLVGLNEKTNSYPHEISGGEQQRVSVARAFVNHPPLLL
                     ADEPTGNIDPETAVGIMQLLVRINRTGTTVVVATHDRDMVNRMRRRVLQLDVGRLVRD
                     ERRGGYDQVAEVEEQEALT"
     gene            23089..24000
                     /gene="ftsX"
                     /locus_tag="VFZ86_11600"
     CDS             23089..24000
                     /gene="ftsX"
                     /locus_tag="VFZ86_11600"
                     /inference="COORDINATES: protein motif:HMM:NF038347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HEX5642965.1"
                     /translation="MRLGFFLGEAFGSMRRNWVMAMAAVITVFISTAILGAVLVTRDN
                     LNQGATSLKNRVMIEVFIRDEATDEEKAALEQRIQSIPEIKKSTYISKEEALERFRKR
                     FGERIVQNLPINPLPASWEIQVNDANQVDAVAAKFFDDPAVDNDPGTHNGVKYARETV
                     RKMLGTINLIGIGMWIATAIFALAAVLLISTTVRLSIFARRREVEIMRLVGATNWFIR
                     WPFVLEGFITGLIGAIVAAGFVWLCNWALSNWLKSSDIDFLNVRVFSMWWQSGSWPAG
                     LLPTLAVLGAVLGALGSMVALRRYLKT"
     gene            24082..25293
                     /locus_tag="VFZ86_11605"
     CDS             24082..25293
                     /locus_tag="VFZ86_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009372206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX5642966.1"
                     /translation="MTSPRRSSRVASFIAALLLAAVTLALAGALATSARAETAAELQQ
                     KLRSARARIEAAKEHKATLNEQISDLDGRLSAIQRKLDGLGGQIAAVEDRLSVTREKL
                     AVLRKELRLKKLELAAAQAELELEQRNFAERAILSYKSDDLGYLDVLLASSDFEDLIS
                     RVNVIHDLLGGNDALVGGLEQARDGVDAEKDAIARREGAVADAAQVLQEKSDELAALR
                     AAQAASQAAALAVRRQKQGALKGVNKDLAELERQEDQLLAESQALAGVIAGSSGSGQG
                     TGSLIYPVNGPVTSPFGWRIHPILRYRKFHTGLDFGVGYGTPIRASDSGTVIYSTWMG
                     GYGNVIIVDHGNGLSTLYAHQSSVAVGSGARVTRGQTVGYVGSTGFSTGPHLHFEVRV
                     NGNPVDPMGYL"
     gene            25346..26527
                     /locus_tag="VFZ86_11610"
     CDS             25346..26527
                     /locus_tag="VFZ86_11610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX5642967.1"
                     /translation="MRALRTIIVVLLVSLGFLGGYFAGWYLHGENTVRLSPTQQTSAK
                     EAGELQQRIIEELQGRYYRPVDVDKLSEAGVNGMLKSLKDPYTVYLTPREVQEFEEKE
                     RGSYSGIGASLQKTEDGLVITSVFDGSPAKAAGLVPGDRILSVDGKSTKSVAIETSIS
                     RIKGEEGTTVVLSIQSKDKTKPASDVRVVRRTIEIPETSKRIIDDKGTKVGYVQLYEF
                     GGLAGRDVRENVDELSKKGAEWFILDLRYNGGGLLTQAVDVTGVFQTGLVTSTAGLHS
                     PEEELTATGPVATDKPMVVLVNGFSASASEIVTGALKDHDRATIIGTKTFGKGLVQSI
                     VDLGNGAALKLTTAVYLTPDGTNINKKGITPDIVAADDPKTKKDDALQRALQYIAAQE
                     K"
     gene            26532..28550
                     /locus_tag="VFZ86_11615"
     CDS             26532..28550
                     /locus_tag="VFZ86_11615"
                     /inference="COORDINATES: protein motif:HMM:NF012975.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HEX5642968.1"
                     /translation="MSPARRPEEPRDRARPLPYRVARWGKFWSLEPLFADQRSYLVAK
                     GGTPPRLDDLVLAVPSHGDRRRIVEVLGGADDVKAVLRALLHAKGVRQGFADAVLEEA
                     AAVKGRAARPDHGRRDLTALPTFTIDPDTARDFDDAISVAREGEGYRAHVHIADVSYF
                     VDDDGEIEREARRRTSSLYLPLFAEPMLPAALSSDLCSLVPRQPRKCVTVEFTFDAGG
                     RRTSTQYYRSLISSDHRLTYGFADTVIGPAGVAAGEGVDDPAGALAAAAPLADPKAAV
                     AALPGAHLVAGRAEPPRDVPDATMAADDALSAQLLLAAELAGVLRRRRLARGALTIGS
                     FEPEYAFDHAGELSGAAARPETPSHALVEEFMLAANEAVAEYLLRKKARTLYRVHEPP
                     EPASTRELLDQLEELGVPTPAFPSGPAVPAAQLQEAYGRLSRLVGEASVREKRGRLAW
                     STLILRSLKQARYGPANLGHFGLASPAYLHFTSPIRRYPDLVTHRALLFHLGEAGSEL
                     GDAELATAADDCSTRERDFSRLELTGDDVALCFLLERRLEAEGWEQMFTGEIVGLVGG
                     GLFLRFGEVFEGFLSSRRLGGERFEVSEHETALVGEATGIRYRLGDPVDVKVERVDRL
                     SGKVDLAPAHPGGGRTATAPGRRPARRTTGSRPPRRRAPGRETQRKNR"
     gene            28613..29101
                     /gene="smpB"
                     /locus_tag="VFZ86_11620"
     CDS             28613..29101
                     /gene="smpB"
                     /locus_tag="VFZ86_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018703025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEX5642969.1"
                     /translation="MTRETGIKRVAENRKARHDYFIDDTFEAGIALVGTEVKSLREGR
                     INLRDTYVEVRGSATSPELFLVGAHISPYDQGNIWNHDPLRARKLLLHRSEIERIAGK
                     VREKGYTVVPTKVYFKDGRAKVEIGLARGKKLYDKRHDLADRDAKRDIERALRGRGED
                     TR"
     gene            29157..>29353
                     /locus_tag="VFZ86_11625"
     CDS             29157..>29353
                     /locus_tag="VFZ86_11625"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF013494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEX5642970.1"
                     /translation="MDTLSQEQIAAALGELDGWGLDGEALTRTFRFADFVHAIGFVEH
                     LAEVAEEQKHHPDIDIRFNKVT"
BASE COUNT         4414 a        10487 c        10377 g         4075 t
ORIGIN      
        1 tacctggccg tcctccgcgg tgaggacgcc tactaccggc gcttcgtcac ccgcctgggc
       61 tgaggctgct ccgcgcgcct gcgtcgccgc tgcccgggtg tccttctgcc cgcctgaggg
      121 aatacatact tgcctgagca accgtcggcc ctggaggatg gtgccggatg cccgcagtca
      181 ctactcccaa gaccttcgtc tgcggggtct gcagcgcgac cctgaagtcg cagcgggacc
      241 tcgacaacca catgtgcctg ctccacgacg attgcggaga ggcgcatctc ggcacgccga
      301 tcacgttccg ctgcgtcacc tgcggggagg cgttcgcgct ccgcagcgac ctcttctccc
      361 accagaagga actgggccac ggccatcccg gcgagtggga caaggctcgg ccggcggggg
      421 cggagcgccc gcgccgccgg cctcagagcc gcggccgctg agcgcggcgc gctcagctca
      481 gcccgagccg cacgcacgcc gagcctcgag atcagtcggc ctcggtcgcg ggcattcccc
      541 aacgcacggc gtgtgacggc gggcccgatg tgggtaagac catcgaccat cgggcggccg
      601 gaggtcggca gcccggcacg tcgacacgga ggtcggccat gaggcgtgtg atcgccatcc
      661 ttgcgttgac cggggccgtg ctcctgctgg cagccgcgcc ggcggtcgcg gccgagtaca
      721 gagtgacgaa ggcatccggc gcgctcgccg gtcgcgtggc gccgggtccg gatcccgggc
      781 agggagaccg gatcggcatc gtgcgcgacc tgagcaagtt caggggcgcc gtctactcgc
      841 ggcagaccag ctcgggctac acgggctggc cggtgtcgaa cgggacgtcc tacgtcgcct
      901 gggtcaccaa gagcagcacc acccggttcc tcgtcaagaa ggttccctgg ggcgcgaccg
      961 ccggccgcgt gaccaggctc tcgaacggac gctggctcgc gcagaagcgg gtggacggcc
     1021 gctggaggac cgtgggcagg atccaggagg gctgcagggg ccagtgggcg gcgggcgccg
     1081 cgcgcctgtt gctctggcag cgctgacggc ttcgccggcg gcgtcgctcc gcacacagcg
     1141 cccggcgcga ggtccccata ccgctcacag gtgttgtcac ttctgacaac gctcccgctt
     1201 cctgcaggcc gcgggactac gacatctgtc cagtcaaagt cacgtagttc ggcagtgtgt
     1261 gtgtcgtcgg gccggggaac accatgctgc acatcttcac gatgcttaac gccagatgaa
     1321 ggcaactaca tgtgagccct ccgagcgggc cgcgggcagt ggttctgccg attcggaagg
     1381 cgatacccaa ctgacggggg aggagagtcc gatggtcgag ttcaccagac gacagttcat
     1441 caagatcggg ggcggcgggc tcgttgggct ctgggtggga tcccagttcg ggggcttcac
     1501 ccaggtggcg gaggccgcca tacccggcgg ctcgctcgac cccgtgaatg tgcccaagtt
     1561 ccagacgccg ctgctcatcc caccggtgat gcccaaggcg gggaccatca agctcaaggg
     1621 cggcaagaac gcggactact acgagatctc gatgaagcag ttcgcccagc agatcctccc
     1681 ggctaccctg cctccgacca cggtctgggg gtacggggcg gtcgctgcgg cgagcaaacg
     1741 gggactcatg atccacaacg caccgtcgct gactatcgaa gcacaagccg gccggccggt
     1801 gcgcgtcaag tggatcaacg gcctggtcgg cacggacggg aactacttgc cgcatctgct
     1861 gccggtcgat ccgacgctgc actgggccaa ccctcccggc gggaccgcgg agcgagacac
     1921 gcggccgatg ttcatgtcga ccccgggccc atacacgggc ccggtgccga tcgtcacaca
     1981 cgttcacggt gccgtgggcg tgggggacga gagtgatggc tacgcggagg cgtggtatct
     2041 gccggccgcg agcaacaccc cagccggctt cgcggatcgt ggcacctggt acgacttctt
     2101 tgccggcaag gcggcggccg gctacggcgc cacctggggt cccggctacg ccaccttcca
     2161 gtatccgaac gacaacaggg cgtcgacgat ctggtaccac gaccacgccc tcggcatgac
     2221 cagggtcaac gtctacgccg gcccggccgg cttctacatc atccgcggtg gtcctgcagg
     2281 ggacggcgcg gtgcttgact cacggaccgg acttgctgca atcctgcctg gcccggcgcc
     2341 gaaggagaac gacacgttcc catccacgaa ggcctactac gagattccaa tcgccatcca
     2401 ggaccgggcc ttcaacgctg acggttcgct cttctatccg gacacacgcg cgttctttga
     2461 cgacgacgcc ggtccgtaca tcccagcctc ggacatctct cccatctgga acccggagtt
     2521 cttcggcaac accatcatgg tgaacggcaa cacctggccg ttcctgaacg tcgagcagcg
     2581 gcgctatcgc ttccgcctgc tgaacggctg ccagtcgcgc ttcctgatcc tcgacttcag
     2641 cgcgatcacg ggcgctgagg tgtggcagat cggcaacgaa ggcggattcc tgtcggctcc
     2701 ggtgaatatc acaggtctgg gcaaccagct gctgatgggc ttggccgagc gcgccgactt
     2761 gatcgtcgac ttcaccaacg tgccggccgg gaactacgtg ctcggcaacg tcggtgcaga
     2821 cgctccgttc ggcggcctcc cgatcgaggg gccggagctc gccgatcccg caagcacggg
     2881 gcagatcatg cagttcaggg tcgggccggt cctggctccg gacgcgacga ccccgccgca
     2941 gttcctggtg ctgccgccac tgacgcccct tccggcgccg gtcaggtcac ggccgctggc
     3001 gctgctcgag atgatgtcga tggcggatcc cgctcgaggg ccagcggctg ccatgctcgg
     3061 caccgtcgac accgtcaacg gaacggcgat gcatcacgaa tggatggccg ccgtcacgga
     3121 gaaccctcag gttggcgaca ctgaggtgtg ggagttctac aacttcacgg ccgacgctca
     3181 cccgatgcac gtgcatgagg tcgtcttcga ggtggtcgac cggcagccca tctccttcac
     3241 cgagggtgcg atgggaccgg agaacgttgc gctggctggg cctgcccggc caccggaagc
     3301 atgggagagc ggcttcaagg acaccgtgac cgcctacccc ggcgaagtga cccggatccg
     3361 cgcccagttt gcgaaatccg gtcagttcgt gtggcactgt cacatcgtcg agcacgagga
     3421 caacgagatg atgcggccgt acaggatcgg tccggagcag ccgggccagc cgatgccgca
     3481 tacgcacacg atgccctgac agcgctagac acctgacgcg ggaacggcgc cggcggccgc
     3541 gaggccgccg gcgccttcgc gtggtgcccg actcgcgcac gctgggatcc gggagaccac
     3601 ccgggctggc cgttcccgtc ggcgactgcg gaagcgagag cgccggccgc cggttcaggt
     3661 gtagattcga agcgcccgcc aaggcgcgcc ggcggcgaag tccaggtact gggtgcggag
     3721 gacctggacc tcgccggcgc ccatgaacgg cccggggatg cggcccttgt ggagccggat
     3781 gtgcaggtag tggcccggcg cgtcggcgac ggcgcggcgg tgggcctcgc ctcggtgcca
     3841 cgcgtcgggc acgtccgggg tccagaagag gatcagattg ccgtacctgc cgtgttccgg
     3901 cccgacgtcc aggtaactga ggaacccgag cggagcgtac cgcgggaaca gctgggcgat
     3961 gcgtctctcg tgctcgaaca ggacggcatg gtcgacgtcc tcccgcaggc gcccgaagaa
     4021 cccgacagcg gtgacgtcgc gcgccctgag cagggcgcgg ccgtcgggta ccgcgagcag
     4081 ccggcgtccg cccgcggagg tctgctcgct gatcccgggg ccgtcgatgt cgggtcccca
     4141 cgtgagcgcg agctcccgct cgagctccag catgcgggac atgacgagca gatcgccctt
     4201 ggtctcttgg gggtccgtga acggcctgtc cggcaggcgg tcttccggcc cgagtacgga
     4261 agcgaacaca ggcgccgccg gccttgcggg caagcggtcg tcctgttgtt ctcgcgtcgc
     4321 catctcctgg gtctcctgga gatgacgtcc cacgcggcag gcgcgccgta aacgccgacg
     4381 ggcgcggagg gaagacggcg aggtacaggc gatggcgtca cgcgccttcg actccgagag
     4441 gagctcctct gcaggctcgg cgacagcgac tcctgctcca tcggccgaat ggaggagggg
     4501 cggcggtgtc gtccgacact gccgccccct cgtcccccgt cgcgggcgtc tcccccctcc
     4561 ggcgcgcccg accgctgcgc tcagagcgcg ttgacgccct tggtgaggtc gttgagcacg
     4621 gcggtcatgg tgtcgatgcg gtcacgcagg tccgcccgca ccgccgcgcg gtcggccctg
     4681 aggcccgcga tcgccccggc gaagtcgccc tcgccgagca tcacgtactc gtcgatgcag
     4741 tcgaccacca gcggcggcgt accgagcgcg ccccaggtga tgtgctcgta gtcggggtag
     4801 tggcgcacca ggtcctcggc gcagacctcg gggctgaaca agctggcgta ccagttgatg
     4861 ccgacccacc agctgatcat ctgctgcgcc cgcgccgcgt tgcgcgtctt ggccgcggtg
     4921 atcgccttcg tcaggtagat cgcgtccgtc gtccccgcct cgtgcgggta ggccgtgaag
     4981 tcgtactgat cgcggctcgt gaacgtcgcg ttgatggtct tctcgagcgc gacgagcttc
     5041 tcgttgacgg cgtcgacctt gcccttgggc gtcttcgcct tgcgcagctc ccactgccac
     5101 gccgccgcgc ggaacttggt ggcggcggtc ttgaagcgcg tcgctgccgc ttcgctcacg
     5161 ccggcgcgca cgagcttgcc cggctggatg gtgcccagca gctggtcgcc gcgggcgcgc
     5221 aggtcgtagg ggagcacgcc ctcgtcgaga ccctggatca cgcggtggtt ccacttggcg
     5281 atgccgccca ggtagtcgtg atcgacgcgg gccttgttct cgaggtcggt gtggtagtag
     5341 cggtcgtact cggggccgcc ggcggaggtc gtcaccgtcg gcacgccgga ggcgttccag
     5401 ctccactggt cggtccaggt gctcggcgta ggctcggcct cgatgccgta gggcagcgcg
     5461 tcgcccgagt ccgcggcgac cgcctcgagg aacggcagca cgtcggcggc cgcgcgcatg
     5521 cggaagtcgg cgcccgtctc ggccatgagc tcgatgttga gcatcatcgc cagcttgccg
     5581 ggccagtcgg gatgctcctg cgtgatcgcg taccaggcgc cgatcgccca gtcgtaccag
     5641 cagtcggtgt agccgtactc ctcggcgcag gtgaagaaga agatgacggt gcgccgcggc
     5701 tggacgccgc tgtccgtcat cgccttggcg gtgagcaact cgttcaccgc ggcgccggtg
     5761 tcgtccaggc cggcgcggaa gtgggcgtcg aggtgggacg cgaggagtac cggcgcgagg
     5821 tccggatcgc tgccttcgag cacgccgacc acgttgtagc cgacgccgcc gtcctcggcc
     5881 agggtgatct tcacgtcgct cgtcatcgtg gccatcagcg acccacccgc cgcggcgagc
     5941 ttcgccttga tccaatcacc gtcccgccag gcgacgtaga ccatcggcac ccagtcgtcg
     6001 tcgtactcgc cgtcgttgct gccgagcgcc tcggggtagc tgtaccacgg gtacgtgttc
     6061 tctccgtagg tcatcacgac cccggcggcg ccgcgatgcg tggcctcggc gccgacgaag
     6121 ctcagccacc agtcgtcgaa ctcgccgtcg accagcacca gcttgccggc cacgatcccg
     6181 gcggcgtcga actcggccgc cgtgccggtc ccgacgtaga cgagctcggc ctcgagcggg
     6241 ccgtcggtgc cgggtacccc ggcgaacgat gaagcggtga gggtctcacc ggcgacctcg
     6301 accgtcgcgc ccttgaagtc ccaggcgtcg acgggaacag cctcgaggcg cacgtcggag
     6361 agccccatgg cctcgagttc gtcgcgcacg aggtacgagg catcgtgctc ggcccacgtg
     6421 ccggcgaggc ggaagcccag ctgcgggtcg gtcccgaggt cggtgagggc gtcttcgatg
     6481 tcggtcgtgt agccgttggc gacgagctcg tcaacggctg cgtcgtaggt cagggcgctc
     6541 gccgccaccg gcaggacgag cgccagcagc aaggccatca ggatgggaag gacgagacgt
     6601 ttcacggtac cccctttgcc gcatgcgcgg cgggcacagg cgcccgccgc ccgtcgcgcc
     6661 ggggatccct tcagacggcc ccggcgatcg tgcacccaac ctaccgatag cctcgagttg
     6721 gggtcaacag cgccgccggg aagtgttgtc cagactgcga acgcccgcga cgggggctct
     6781 cgtcaccctt ccagcagacg cagcgcggcc gcctcgatgt ccgcggacgc gatcccggcc
     6841 gcggccatga gctcggccgg cgtccccgac cccgggagcc cgcggaccgc gaggtgctcg
     6901 acgcggaact cctcgcgcag cggggcgagg gcggcaagca cggcttctcc caggccgccc
     6961 tccgggtagt ggtcctcgac gacgacgatg cggcgcccgg cttcgcgcgc cgccgcggcc
     7021 aggccgtccg catcgagggg cttgaccgag tagaggtcga tcacccgggc cgggatgccg
     7081 cgttcgcgga gctgctccgc ggccgcgagg caggtgtgca acgtcacccc ggcgcccgcg
     7141 agcaggacgc ggtcgtcggc ggaggcgtgc aggagcttgg agccgccgac cgggaactcc
     7201 tcgtcgctcg ggtagagcac ggggtaggcg ccgcgggtgg tgcgcaggta gctcacgccg
     7261 cccagctcgg ccatggtggc ggtgagcgcc gccgccgagg tggcgtcgct gggatagagc
     7321 accgtcgacc cgtgcacggc gcgcagggcc gcgaggtcct cgaggcccat ctgcgagggc
     7381 ccgtcctgcc cgatctccac gccggcgtgc gagccgcaca ggcggatgtc cgcaccggag
     7441 acggcggcca tgcggatgaa gtcgtaggcc cgcgtgagga acgccgcgaa ggtcgccgcg
     7501 aacgggatgt agccgcgcac gctcatgccc acggcggtcc cgatcatctg ctgctcggcg
     7561 atgtacatct ggaagaaccg gtccggatgc gcgtcccgga acttctcggt gaaggtggag
     7621 tttccgacct ccccgtcgat ggccacgacc tcgggccacg cgccgagcgc cgcgagggcg
     7681 tcgccgaacg cggcgcgtgt cgcgaccttc atgcccatct cgtacttcgg cagggcgagc
     7741 gcgcgccgct cgcgcaccgc cgccgccggc cgctccgggc gcggcggcgt ctcgacccgg
     7801 tggtcgctca cgccgccgag ggcgcgcacg gcggcctccg cgacgtcctc cttcagcggt
     7861 ttgccgtgcc agccgacggc gtcctccacc tcgggcacgc cctttccctt caccgtgcgc
     7921 gcgagcacga ccgtgggcag ctgcgagcgc gccgccagtg acagggcgtc gtcgatctgc
     7981 tcgagatcgt gcccgtccac gacgagggcg cggcagccga aggcctcgac gcgccggcgg
     8041 tacgcctcaa ggtcccactc cagcccggtg ggccccgtct gcccgagccg gttgacgtcg
     8101 acgatcaccg tgaggttgtc cagctcgtag agcgacgcct tgtcgagggc ctcccacatc
     8161 gagccctcgg ccatctcgct gtccccgcag agcacccaga cgcggaaatc ggccctatcc
     8221 agccgcttgc cggccagcgc gacgccgacg ccgtcgggca gcccctgccc cagcgagccg
     8281 gtggcgacgt cgacccaggg gagcgccggc gtcgggtggc cctgcaaccg cgaccccgcc
     8341 cgccggtagg tctccaggag ctcgcggtcg tcgacgacgc cgactgcctt gtagagggag
     8401 tagaggagcg gtgaagcatg tcccttggag aagatcaggt ggtcgttgtc ggggcggtcc
     8461 ggccggctcc agtcgtagtg gaggtgccgg acgaacagca cggccatgag gtcggccgcg
     8521 gagaggctgg aggtggggtg gccggaaccg gccgaagcca cgctgcggat gctgtccacg
     8581 cgcagttgcc gcgccagctc gccggcggcc tcgagggccg gccgggcggc gtctgtggga
     8641 agggcgctca tcgtcgatct cctcgtcgtt ctcgtcgctg cgcgactgcc ttggcgaccc
     8701 cccgccttgc ctgttgcttt cccgcgaggg gcgacggcat gcaggcgggg agttgcgccg
     8761 cgggcgctcc cggccggaca ctgtcggagc gacggcggcg gcgttttggc ggcccgcgac
     8821 cgggcaggtg caaggcagac gggcggcgcg gcaggaccct cctctgccgg caccgcccgt
     8881 ctggacgagg cggcgaggcg atcggacggc agatggcgat cctgtatgcg ggtacatcgg
     8941 ggtacgcgta cccctcgtgg aaaccggagt tctatcccgc cgacctcccg gccgcgcggt
     9001 tcctgcgtca ctacgccggg cggctcaact gcgtggagat caactacacc ttccggcgca
     9061 tgccgtcgga gaaggccctc accgcgtggg cggaggccac gccgcccggc ttcgccttcg
     9121 ccgtcaaggc gcaccaggcg atcacccaca ggaaccggct caaggagggc gcccgcgagc
     9181 cgttggagta cttcctcggg acgcttgcgc cgctgcgtga cgcggggcgg ctcggcccgg
     9241 tgctgctgca actgccgccc aatctcaagc tcgacctcga gcgtctggag cgcttcctcg
     9301 agctgctccc gcgcgagctg cgcttcgcat tcgagttccg agaggcgtcg tggttccgcg
     9361 aggaggtcta cgcgctcctg cgcgcgcacg gcgccgcgct ctgcgtggcc gaggctgagg
     9421 agctgacggt cccggacgtg gtgaccgccg gcttcgccta ctaccgcatg cgcaagcctc
     9481 cctacgacga ggccgccctg gcgagccttc gccgccgcgc cgaaggactg accggcggcg
     9541 gccgcgacgt ctacgtcgtc ttcaagcacg aggaagaggc gggcggcgcg ctcgaggcgg
     9601 agcggctgct cgccggctga gagcgcggcc cggtggccga gccgcgaccg ccgtgcgcca
     9661 ctcggcccgt gcaggctagg atggggccgt agcccgtcac acactgggag gacacgtgca
     9721 gtacgaggag ttcctcgatc tcgtcggcag ccgccggacg atccgcgcca tcaagccgga
     9781 cccggtgccg gacgacgtgg tgcgcaagct gctcgaggcg gcgcgctggg cgccgaccgg
     9841 cttcaacatg caacccgtgg aactgctggt ggtcgcggac gccggactcc gtgcgtcggt
     9901 ccggagcatc gtcgacgact acaagaacgc cgacttcttc gccctcgagg cgacgcgcga
     9961 ggagtggcag gggtcgccct ggtctgccga gacgcacggc ccgtggcgct gccccgaggc
    10021 gccggtgatg atcgccgtgc tcggcgacac ccgccggcgc gtcggcctgc ccatggccgc
    10081 gcgctacgcg acccagaagg gcgactccat cttcgagtcg tcgctcgcca acgcgttcct
    10141 gtacctcctg ctggccgcgc acagcctcgg gctcgcggcc cagcccgtct ccggggtcaa
    10201 gtacccctcc gtgcagggcc gcgtgaagca cctcctgaac ctcccggact tcatctacgt
    10261 gtacgacctc gtgctcgccg gctggtcggc gatggacaag ccggtcgccg gcaagctgac
    10321 gcggagcctg gacgagatgg tccactacga ccgggccact gacgacgagt tcccgagcga
    10381 ggaagagata cgccgccaga tcgtgaggct gcgcgccggc aacgtggcac gtcacgtgga
    10441 gggcgacgag gcaggcggct gaggtccccg gcgtccttgc acgttcttcg ccaattcgta
    10501 acgaatgtgc ggtatggttt caaacgaccg tcacggacca gcgattgttg aagcgaggca
    10561 ggcgtcatgg accccagtga gttccacgcc ccgggtgccg gcaagatcgt gacctccccg
    10621 gagggctacg ccgccttcgt gccggcgccg ctccctccgc ggatctccta caccaaggac
    10681 ctcgtgctgg ccctgtcgcg cgccgacgcc gcgctcggcg agctctccgg cgtcggcggc
    10741 gagctgcccg acccgcagct gctcgacgcc tcgttcaagc gccaggaggc gctctgctcg
    10801 tcgcgcatcg agggtgcgca ggtgagcctc tccgacctct acctcgacca ggtcggcgcc
    10861 gcgcgtcaga ccgtggagcg cgagcacctg cgagaggtgc gcgcctgcat ctcgaccctg
    10921 cgcttcggcg tggaactgct gcgggaagag ccgctctccc tggagttcgt caaggcgctg
    10981 cacgagcggc tcctgcgcgg tgaggtgggc ggcgcccgca cgccgggcga gttccgcacc
    11041 tcgcagaact ggctcgggcc gcccggcgcc accatggcca cggccaccta cgtgccggcc
    11101 ccggtcgacc agctgccgga ggcgctggcc gcgttcgagc agttcctgca ggaacgcggc
    11161 cggctgccgg acctcatcca gtgcgccatg ctccacgcgc agttcgagag catccacccg
    11221 ttcgtgggcg gcaacggacg tctgggccgc ctgctcatca cgctgttcct catcgagcga
    11281 gagcggctga cgcgcccgct cctgtacctg tcggcgttcc tggagggaca ccgggacgag
    11341 tactacgcgc tgctgcagcg cgtgcgcacg cacaacgagt ggacgccgtg gctcacgttc
    11401 ttcgccgtcg gcgtgcacga gaccgccgag cgcgcgaccc gccaggcgcg cgcgctcatc
    11461 cgccactacg agcgcgaccg cgagaaggtc aagggccacg ccctgcgcct ggcggacgag
    11521 ctcttccgca cgccggcgat gacggtgccc gaggccaagc ggatcctcga ggtgacggac
    11581 cccacggcac gccgcgcggt ccgcgagctc gaggcgcgcg gcctgctcgc ggagtggtcg
    11641 gagaagcggt ggccgcgcgt ctacctcgcg cggcacgtgc tggacgccgt gctgcacccg
    11701 ctggaagacc tgcgcagcgg gccgggcacg gccgatctgg gcacggtggt caagtcggag
    11761 gcgacgcagg cgcaggcgaa gcccgcgcgc cgcgccgacc gcctcatggc ggaggccatg
    11821 gacaccatcg acgcggcgcg cgccaggggc gtgcagctgc gcctcaccgg cgggctcgcc
    11881 gtgcggcgct actgcatcga cctcgacttc atggaccgcg agtactccga catcgacttc
    11941 gtcggccgct cggaccagaa gaaggagatc cgcgacgtct tcgagacgct cgactacacg
    12001 gagaaccgct acgtctctca gtccaccgac tccgggcagc tgcagtacat caagaacgag
    12061 gccctcgagg gcatgaaggc cgagtcgaag gcggccgcgg ccggcgagcg cagcacgtac
    12121 gagccaccgc tcgtcgacca catcgacatc ttcctcgacg tcatgcgcat ggaccacgac
    12181 atcgacgtcg gcgagcggct ggagatcgac gactacgcga tctcgccggc cgacgcgttc
    12241 atcgccaaga tgcagatcgg caagatcaac cagaaggacg tccacgacgt catcgctctg
    12301 gtgaaggacg tgccgctgcg ggacgtggac gacgacgcct cgatcgacct gctctacatc
    12361 gccgaggtct gctcgcacga ctgggggctg taccaggaca tcaccaccaa cctcgacatc
    12421 gccctggcga tggtcgacga ctacgggctc acggaggcac agcaggaccg cgtgtacgcg
    12481 cggctcgcgg ccatcaagga gtcggtcgag agcgcgagca agacgctccg ctggcgcctc
    12541 cgggccacgg tgggcgatcg ggtcgcctgg cggcgcgaga tcgaggacac ggaggggacg
    12601 cagatcatcg cgccggagtg ggactggcgg cgcgacctgg gctagacaag gggggcaggg
    12661 atgacgggca tggtcccacg ggacgtgtac gagctgacgg cggtcgcgga cccgcggctc
    12721 tcgccggacg ggacgacggt ggcctacgtg gccggctccg tcgacgagaa ggggaacgcg
    12781 taccccgccg cggtctggct ggcggcggcc gacgggtcgg cgccgccgcg cgcgttcacc
    12841 agcggggaga aggccgacgc cgacccgcgc tggtcgccgg acggcacgcg cctcgccttc
    12901 acctccgatc gcgcggacaa ggcttcgcag ctgtacgtga tgccggtggc cgggggcgag
    12961 ccgcgcaagc tcacgagcct caaggaggac gtgacgcagg cggcgtggtc gccggacggc
    13021 acgcgtatcg ccttcgtcgc ccgcgtgcgc gacgcggcct acgaggaaga ggacgacaag
    13081 aagcggcagc cgcgccgtct cacgcgcctg cagtacaagc tcgacagcgt ggggtggacg
    13141 gcggaccggc cgcagcacct gttcgtcgtg ccggcggacg gctcggccga gccggagcag
    13201 gtcacgcacg gcgactacga ggaccactcg ccgtgctggt cgcccgacgg gcggacgctc
    13261 gccttcgtct cggcccgtca cgaggactgg gacctcgaga tggtcaacga cgtgtacctg
    13321 gtggacgccg ccggcgggga gccgcggcgg ctcacgcagg gcggcggcag catcggcagc
    13381 gtgtcctggg cgccggacgg gtcccggctc gccgtcgagc gctacgccgg cgtctacgac
    13441 gacccgaagc acaccgacat cgcgctggtc gaccctgcgg acggcgcgat cacgccgctc
    13501 accgcttccc tcgacctgaa ctgctcgacc taccccggga tgcgcgagcc gctctgggac
    13561 ggcgacggcc tcgtcttcgt cgccgaggac cacgggcgcg tgcacgtgta ccgcgtgccc
    13621 gcggacggct ccggcgagcc gcgcctgatc gccggcggcg agcgcgtcgt gaccgggctg
    13681 gacgtggccg gcagccggct cgtcttcacg gcaagcgagc cggcgcggtt gagcgagctg
    13741 tacgacggcg gtccggcggc cggcggggat gaggacccgg ccgccgtggg ccggcggctc
    13801 acctccgccg gcgacgcctt cgccgccggg cgcgagctgg tgcgacccga gcgcttcacc
    13861 gccgtctcgg cggacggcag ccaggtcgac gcctggatca tgcggcccgc cggctacgaa
    13921 gaggggaaga agtacccgac cctcctcaac atccacggcg ggccgtacgg ccagtacggc
    13981 gtcggcttct tcgacgagtt ccaggtctac tgcggcgccg gctacgccgt cgtcttcagc
    14041 aacccgcgcg gctcctccgg cggcaccgag gcgtgggcgc gggccatccg cggccccggc
    14101 gacgagggcc ccggctgggg ctccgtggac tacgaggact gcatggccgc cgtggacgag
    14161 gcggtccgcc gcttcgactt cgtcgacgcg gaccgcctcg gcgtcatcgg cggctcctac
    14221 ggcggctaca tgacctcgtg gatcgtgggc cacaccgacc gcttcaaggc cgccgtctcc
    14281 gagcgcgccg tcaacaactt cgactcgcag tggggctcca gtgacttcgg ctgggacttc
    14341 aagggctaca cgggcacgtt cctgtacgag gacctggacc tgtaccgggg ggtctctccc
    14401 gtcacccacg ccgagaacat ccacactccg ctgctcatcc tccattccga ggaggacctg
    14461 cgctgtcccg tggagcaggc cgagcacctc ttcgtgacgc tgcgcctgct caagcggccg
    14521 gtggagctcg tgcgcttccc cgccgagtcg cacgagctca cgcgctccgg cagccccctc
    14581 catcgcgtgc agcgcttcga cctggtggtg gagtggttcg accgccacct caagggctga
    14641 ggcgggaaga cgatggacgc ggcggcattc ggcgagcggg ccagcgggca cctggtgacg
    14701 gccgagggcg gccacccggc gttcatgccg gcgccgctgc cgccggagct gacgttcacg
    14761 gcggagctgg ccgagcgcct ctccgctgcg gacgcggcgc tcggcgagct ctccggcctc
    14821 gccgggggac ggcgcgaccc gcagatcctc gtcgcgccgt tcctgcgcca ggaggccgtg
    14881 ctctcctcgc gcatcgaggg cacgccggcc acgctcgtcg accttctctt cgacgaggtc
    14941 gcggctgcgc ccgacctcga gctgcgcgag agccttcgtg aggtgcgcaa ctacgtggcc
    15001 gcgctgcagt acggcgtcga gcggctcgcg gacgtgccgc tgggcaccac cctcgtgctc
    15061 gagctgcacg agcggctgct ccgcggcgtc cgcgacgccg cctggacgcc gggcgagttc
    15121 cgcagcggcc agaactggat cgggccgccg ggctcgaccc tcgagacggc ggtctacgtg
    15181 ccgccgccgg tgccggagat gcacgcggcc ctgaccgaat gggacaggtt cctgggagag
    15241 cgcaccggcc tgccgccgct cgtgcagtgc gcgctcatgc acgagcgctt cgaggccatc
    15301 cacccgttcc tcgagggcaa cggccgtctc ggccggctgc tcatcacgct gttcctcatc
    15361 gagcgcggcc gcctctctca cccgctgctc tacccctccg cctacatcga ggcccaccgc
    15421 gacgagtact acgaccggct gcaggcggtg cgcaccgacg gcgcctggga gccgtggctc
    15481 ctcttcttcc tcgacgccgt gcgcgagacg gccgagcgcg cctccgccca ggtgcgtgcg
    15541 ctcatggcgc ttcgcgagca gtaccgctgg aaggtctcgg ggtacgcgcg ggaactggtg
    15601 gacgacctct tccgcacgcc gttcgtcacc gtccccgagg cgcagcaggc gctcggcgtc
    15661 agcaacgcca cggcgcgcaa ggcggtccgc gagctgcagg agtgggggat gctcgaggag
    15721 ctggccgcgc gacgctggcc gcgcgcctac atcgcgcggc ccatcctcga cgccatccag
    15781 gggccgctcg aggacctgcg cctgaggtcc gaggctcagt ccaggagcgc gccgctcaag
    15841 tcggtgaccg acgtggagga gccgcccgag aagccggccg agcgtcacat ggccgaggcc
    15901 atggccctca tcgacgaggc gcgccgctac ggcgtgcagg tgcgtctcat gggcggcctc
    15961 gccgtgcgcc gctactgcac cgacctcggc ttcatggacc gcgagtactc ggacatcgac
    16021 ctcgtgggcc tgtcggtgca gaaccgggag ctgcacgagg tcctcacgcg gctcgggtac
    16081 gccgagaacc gggcggtgac ggaggcgacg ggcgccggcc agctccagta cgtcaagacc
    16141 caggtgctgg agggcgccgg cggcgcctac cgcatcggcg ccgggcagcg ggacgaccgc
    16201 ccggcgcccc tcgtcgacca cgtcgacctg ttcatggacg tcatgcgcat ggaccacgac
    16261 ctcgacgtac gcgaccgcct gctcgtcgac gactacgcga tctcgccggc ggacgcgttc
    16321 atcggcaagc tgcagatcgg gcgcctcaac gccaaggacg ctcacgacgt gatcgccctg
    16381 ctcaaggacg tcccgctgcg ccaggtcgac gacgagacct cgatctgcgt gccgtacgtg
    16441 gcggagacct gcgccggcga ctggggcctc tgccaggacg tgctcgccaa catcgacgcg
    16501 gtcctgcaga tggtcgacga ctacgacctg accgaggagg aggcggcgcg cgtctacggc
    16561 cggctggcgg cgctccgcga ggcgatcgag gctgaggaga agtccgtggg ctggcgcctc
    16621 cgtgcgcgcg tcggcaggcg ggtcgcgtgg cggcgcacga tcgaggacac cgaagggacg
    16681 gacgtcatcg cgccggagtg ggactggcgg cgggacctgg gctgacgctt ccgcctccgg
    16741 ccgacgaccg accggcgcgg cacggagggc gtacactgtg cgcgtgggcc cgcgagcgaa
    16801 aggcggcagg ctgtggcgta cgtgctgatc gagcaccggg tcggtgactt cgagaccttc
    16861 aggagtgtgt acctggacga cgccgcgcgc cgcgggcggc tcggctccag aggcggccgt
    16921 gtcttccgcg tcgccgacga cccgggcaac gtcatcgtca tcctggagtg ggacaccgtg
    16981 gagcacgccc gcgacttcgc cggctctctc gagttggagc aggcgatgga gtggtcggcc
    17041 agcgatgtct cgacgccgcg ggtgactgtc ctcgacgtgg ccatcgagtc gccgcgctga
    17101 gcggcgagaa aggcggcagg catggtgtac gtggtcctgg agcatgagat ccacgacttc
    17161 gagacgctca agcaggtcta caccgacgac gccgagcgtc gccggcgcat gggctgccgg
    17221 ggcgggcgca tctggcgcgg cgccgacgac cccaacatgg tctcggtgat catggagtgg
    17281 gacaccgtgg agaacgcgcg cgagttcgcc ggctcgttcg agctggagca ggcgatgcac
    17341 tgggcgaccg tcaaggcgcc gaccacgcgc gtcacggtct atcaggaggc gcacagctcg
    17401 gcgtactgac cggtccccgc ggtctggcgc cgccgggccg gcgcgtcctg cgcccgtttc
    17461 gggcccgtct ccccgtgaaa tgacgggagc gaagacggcg gggacgtcac gcccgccgca
    17521 ggcgaacgag gaggaccacc atggccgacg agtatctgcc cgacgtgtac ctgggcttcc
    17581 gtgaacacta tccggccgtg gccgaggcgc tcgacggcct cggcgaggcc gtggacgggg
    17641 cgggaccgct cggcgagcgc gacgtgcgcc tcgtcaagct cggcctcgcc gtcggggcgc
    17701 tggccgaggg cgcggtgaag tcgaacgcgc gcaaggcgct ggcggccggc gcgtcgccgg
    17761 acgaggtgcg gcaggtcggc atcctcgcga tcacgacctg cgggttcccg acggccatcg
    17821 ccggcctcgg ctggatcgac gaggtcctca agcccggctg agccgcggcc cgcctcgcgg
    17881 cggccgcggc ctcaccagcc gtggcggccg ccgtcgcgcg cgaccttctc ctcgcagatc
    17941 cgcgtcatcg cggcgcgcac ggcgcgcgcg gccagccagc cgacgggcgt ggccgggccg
    18001 gcgtagccga gctcctcgcc gcggccggtg caggctacga cgacggtgtc ggtggacgtc
    18061 ccgctcgccg cgtctccctc cggggtcttc acgtcccacg cggcgagggt catggtcttg
    18121 gcctccgtcg cggtgatgac ggcgttgacc atcgccgccg gcgtcagcgc gccgtcgacg
    18181 agcaggacga tgttgatggt gcccgggccc ggcgccgcgg ccccgcccgc cgggccagcc
    18241 ccgatcggcg gggtcacgcc cgccgacgac gtgttgctca gccccacgga gacgacggcg
    18301 gccaccgcga ggtcgcccag ccgctccacg gcgcagcggg cgaactgcgt gtacgccgcc
    18361 gtcatgagcc cgacgaacgg cccgtggatg ccgagctcgg cggcgacggc ggcgaggtcc
    18421 tcgtccggcc gctcgccgtc gtactggtcg tccacgtgca cgttgaggat ctcgcgcgtc
    18481 acgccgaacc cgccgcccac cacggccgag ccgagcaccc gcagcgggcg ctcgctcgag
    18541 atgcgcacgg cccgctcggc ggcggtgacg gtgacgccgg gcagcggctg gagatcgtcg
    18601 ttcacgtgtc cttctctcgg gcgcggggcg gagcgcccgc catcctaccg cacgctccgg
    18661 cgtcctcggt ccgaggtttg agctcccggc gagcggcgaa acgtcaagta gcccgggcac
    18721 gtctccgagg gcccggacac aaggagtaca cgatgccact gttgacagga tccatcgagg
    18781 ccgacgtccc ggtctcgttc gcagaccgcg agtggagaga gttcatcggc cggtcggtct
    18841 acgaccgctt ccccgaaggg tacgaggacc tgcgttcgtc gctcgccgag ctggacgccg
    18901 acgacggccg ggtcaccatc gccgccctgg gcacagggag ggcgcgggtc gacgtcgaga
    18961 tcgactacac cccgcgcgac cccgccgacc cggggagcga cgtcgcgcgc gcccagcgcg
    19021 agctggaccg tgacctggac aagtaccgcg acttcgtgct gcgccgttgc gacgaggagc
    19081 tctgccgccg caactgagcg agccgcgcgg gcgggcggcc ggcgagcttc gccgcccgcc
    19141 cgcgcgccgt cggaggggcc tctccgactc cgcccgcctc tcccgtgctt acgatcgctc
    19201 acctgggtat aaggggactg aatcagtccc ctttaccgga gtgaggtgac ccatgagtcg
    19261 tggcggagcg cccgcgttcg cggatcggcc caacctgtcc gtcctgctcc aggaaccgcg
    19321 ctacgaggtg atccccgtac aggggatcga gcagaaggtc gccgccctgc cgtacggcgc
    19381 caccgtcacc gtcaccgcgt cgcccgcgca gggcctcgag cgcacgctgg acgtgagcac
    19441 cgctctcgcc gcgcgcggct atcgcgtggt gccgcacttc gccgcgcgga tggtaacggg
    19501 gcgcggtcac ctcgagcggc tggtgcagcg cctcgagggg accgagatcc gcgaggtgtt
    19561 cgtgatcggc ggcgacgccg acccgccggc gggcgactac gccgacgccg gggacctgct
    19621 ggaagaactc gtgggcatgg cgcacccctt cgcgcgtatc ggcatcggcg gctatccgga
    19681 gggccacccg ctcgtgccgg acgaccggct gctcgaggcg ctcctgcgca agcagccgtt
    19741 cgccagtcac atcgtgaccc agctctgctt cgacgcgggc gccctggccg cgtgggtggc
    19801 ggacatccgc gccgcaggcg tcgagatgcc ggtggtcgtc ggtctgcccg gcgtggtgga
    19861 gcgtcgcaag ctcgccgaga tctcgctgcg caccggcgtg gggtcgtcgc tcaggtacct
    19921 gcgcaagcac ggacgccaga tcgtgaccct ggcgcgctcg cgccggtacg acccgacccc
    19981 actcgccgag gctgtggcgg accacgccgc ggacgaggcc ctcgggatcc gcggcgtgca
    20041 cctcttcacg ttcaaccagg tggagcagac ccgggactgg gtggccgggc tggcgtagcc
    20101 acacggacgc gtcaggggac cggcgggccc gcctccagtt catcccgcgg cggcggcccc
    20161 gtcgcgcgca gcgaccgcgc cgtggcgagc agcgagccgg ccaggatcag cggcatgccg
    20221 atggcgatcc ccagcgtgaa gggctcgctc aggagcgcca ccccgagcag gacggcgacc
    20281 agcgggttga cgtaggtgat caccgtggtg cgcgccggcc ccacctcacg gatcagcgcg
    20341 aagaagagaa ggaacgccac cgcggtgcac accacggcca gcaccgcgac cgagccgatg
    20401 gtctccgccg agagcgacgc cggcatggtc gcgatgccgg ggggcgcgta cagcaccgcg
    20461 gtcaccacca gcgacgccgc gatcacgccg atcgccggca ggtccgcgag ccggcggctg
    20521 atgatgatcg gtccgaccgc gtagcagaag gccacgacga tcgtctcggc gatccccagc
    20581 gcgtcgctgg aggcgatgtc caggccgacg agggcgacga cgccggcgaa cccgacgagg
    20641 agcccggtga gccggcgcgc gtcgaagtgg tcggccaccc cgaaggcgcg gtacagcacc
    20701 gcgccgatca ggggcacggt ggcgatcagc agtcccgcca gcgagctcga gatgcgctcc
    20761 tcggcgtgcg cgagcagcag ccacgggatg cagagctcga cgagcgtgta cagcagcacc
    20821 cagggccagc tgcgcagcac gcggcggacc tctccgcgcc gcagcgcgaa ggggaggagc
    20881 aggagcgccg ccggcgccgt gcgcaagaag acgagcgccg gcggcggcag ctcgcgcacg
    20941 gcgatgcgga tgaacaggta cggcactccc cagatcacgc agagggagat gaacagcagc
    21001 cagcctttgc gcgacaccgg gggtccttcc gtcggacgtg cgccgcccat cctatcgcgt
    21061 gccggcgcgg cgcccggcag gacggagcgg cccgcggcgc gatacagtgg cggagcgttc
    21121 ccggccttca ccggggtcga caaggcgacg gtcagcgacc ggcagaggag acgtcatgga
    21181 tgtgttcgag gccatcgaga agcggtacag cgtgcgcggg tacaagcccg acgccgtcga
    21241 ggacgagaag ctcgccagcg tgctggaggc ggcgcggcgc gccccgaccg ccgccaaccg
    21301 gcagccgttc cgcatgctcg tcgtgaagac gcagggccgg gaggaggagc tggcgagggt
    21361 ctacggccgc cgctggctca cgcaggctcc gctcgtgatc gccttcgtcg cggtgccggg
    21421 agaggcctgg cggcgcatgg acgacaagcc ctacgacgag gtcgacgcca ccatcgccat
    21481 ggaccacctc gtgctcgcgg ccaccgccct cggtctcggc acctgctgga tcgccgcgtt
    21541 cgacccggac gccgcccgcg aggtcttcgg cctccccgac gacgtcgagc tggtcgcgct
    21601 cacgccgctc ggctaccccg acaaagcgcc ggccgccact ggacgacgcc cgcttcagga
    21661 gctcgtgcgc tacgagcgct ggtgacgccc ggcgactgcc ggcctcgcct cccgggtagg
    21721 agcagggtgt agcgcgcagt cggccgccac ggggaggcgg gcatgggatt cgtggacgag
    21781 accatcatcg tggccgtgga cgtcgccgac gcctactctc tctggctcga ctacgagcag
    21841 tacccgcgct tcatggaggg catcgacgaa gtggccgtcg tcgggtactg ccgcctgcgc
    21901 tggcgcggcg tggtgtgcgg cgagaccctg gtgtgggaga gcgacgtcgt cgaccgcatc
    21961 caggacacgc gcctgcgctg gcgcgcggtg gacggtcgcg agaccggcga cgtcgacgtc
    22021 cagaagctgg accatcgcca gacgctcgtc cacttccagc tcgagtacga tcccacggtg
    22081 tggggcgtcg accccgtccg tctcgaggag tgcctgcgga cgcgcgtccg caacgccctc
    22141 agcgcgttca aggaactggc ggaacgcgcc gtggcgtagg gtgccgcggc aggaacgtcg
    22201 cgccccgcgg cgaacccttg ccacggggtc gccggccata ccagtgctca ttggctcgct
    22261 ccactggtcc ttctcagctc acggcggccc ctgttttccg tgccgggttg aggccttgtg
    22321 aagcggccct cataatcgtc acttggccgt tgacggctga caagggagcg ctaatcccat
    22381 gatcgtcttc gaagacgtca ccaagatcta cgacccggac gtggtcggcc tccaggactt
    22441 cacctgccac atcggcaaag gtgagttcgt gttcctggtg ggcgcctccg gttccggcaa
    22501 atccacgctg atcaagctgg tgctcaagga gctcgtgccc gactcgggtc gcatcctcgt
    22561 ggccggccgc aacctcggca ccctgcggca cagcaaggtg ccgctgctgc ggcgcaacat
    22621 cggctgcgtg ttccaggact tcaagctgct gccgaacaag acggtctacg acaacgtggc
    22681 gtacgcgctc gaggtcgtcg gcgagccccg gcgcagcatc cgccgcaagg tcccggagat
    22741 cctcagcctg gtcggcctca acgagaagac caacagctac ccgcacgaga tcagcggcgg
    22801 cgagcagcag cgcgtcagcg tggcgcgggc gttcgtgaac cacccgccgc tgctcctcgc
    22861 cgacgagccg accggcaaca tcgatcccga gacggcggtc ggcatcatgc agctgctcgt
    22921 gcgcatcaac cgcacgggca cgactgtcgt cgtcgccacc cacgaccgcg acatggtcaa
    22981 ccgcatgcgc cggcgcgtcc tgcagctcga cgtcgggcgc ctggtgcgcg acgagcgccg
    23041 cggcggctac gaccaggtcg ccgaggtcga ggagcaggag gcgctcacgt gaggctcggt
    23101 ttcttcctcg gcgaggcgtt cggctcgatg cgccgcaact gggtcatggc catggcggcg
    23161 gtgatcaccg tgttcatcag cacggccatc ctcggcgcgg tcctcgtgac gcgcgacaac
    23221 ctcaaccagg gcgcgaccag cctcaagaac cgcgtcatga tcgaggtctt catcagggac
    23281 gaggccaccg acgaggagaa ggcggccctc gaacagagga tccagtcgat ccccgagatc
    23341 aagaagtcca cgtacatcag caaggaagag gctctcgagc gcttccgcaa gcggttcggc
    23401 gagcgcatcg tccagaacct gcccatcaac ccgctgccgg cgagctggga gatccaggtc
    23461 aacgacgcca accaggtgga cgcggtggcg gccaagttct tcgacgaccc ggcggtcgac
    23521 aacgacccgg gcacccacaa cggggtgaag tacgcgcggg agacggtgcg caagatgctc
    23581 ggcaccatca acctgatcgg catcgggatg tggatcgcca ccgcgatctt cgcgctcgcg
    23641 gcggtgctgc tcatcagcac caccgtgcgc ctcagtatct tcgcgcggcg gcgcgaggtc
    23701 gagatcatgc ggctggtggg ggcgaccaac tggttcatcc gctggccctt cgtgctcgag
    23761 ggcttcatca ccggcctgat cggcgcgatc gtcgccgccg ggttcgtctg gctgtgcaac
    23821 tgggccctca gcaactggct caagagcagc gacatcgact tcctcaacgt ccgggtcttc
    23881 agcatgtggt ggcagagcgg ctcctggccg gcgggcctgc tgcccacgct cgcggtgctc
    23941 ggcgcggtgc tcggcgccct gggcagcatg gtggctctgc ggcgctacct caagacgtga
    24001 ggagcgcggc ggctcgccgc tgagcgaggc cccggggtgc gagaggacgc cgcgctcagc
    24061 cgccgaactc tactcactgc catgaccagt ccgcgcagat cctccagggt cgcctcattc
    24121 atcgccgctc tcctcctcgc ggcggtcacc ctggcgctcg ccggggcgct cgccacgagc
    24181 gcgcgcgccg agacggccgc cgagctgcag cagaagcttc gctccgcgcg cgccaggatc
    24241 gaggccgcca aggagcacaa ggcgacgctg aacgagcaga tcagcgacct cgacggcagg
    24301 ctgtcggcga tccagaggaa gctcgacggc ctgggcggcc agatcgccgc cgtcgaggac
    24361 agactctcgg tcacgcgcga gaagctcgcc gtgctgcgca aggagttgcg cctcaagaag
    24421 ctcgagctcg cggcggcgca ggccgagctc gagctcgagc agcgcaactt cgccgagcgc
    24481 gccatcctga gctacaagag cgacgacctc ggctacctcg acgtcctcct cgcgtcgtcc
    24541 gacttcgagg acctcatcag ccgcgtcaac gtgatccacg acctcctggg cggcaacgac
    24601 gccctcgtcg gcggcctcga gcaggcccgc gacggggtgg acgccgagaa ggacgccatc
    24661 gccaggcgcg agggcgccgt cgccgacgcc gcccaggtcc tgcaggagaa gagcgacgag
    24721 ctcgccgccc tgcgcgccgc ccaggcggcg agccaggcgg ccgccctcgc cgtgcgccgc
    24781 cagaagcagg gcgcgctcaa gggcgtgaac aaggacctcg ccgagctcga gcgccaggag
    24841 gaccagctcc tcgccgagag tcaggcgctc gccggcgtca tcgccgggtc ctcgggcagc
    24901 ggccagggca cggggagcct catctacccg gtgaacgggc cggtcacgtc gccgttcggc
    24961 tggcgcatcc acccgatcct ccgctacagg aagttccaca caggcctcga cttcggcgtg
    25021 ggctacggta cgcccatccg cgcctccgac agcggcaccg tcatctactc cacgtggatg
    25081 ggcggctacg gcaacgtgat catcgtggac cacggcaacg gcctgtccac gctgtacgcg
    25141 caccagtcga gcgtggccgt ggggagcgga gcacgcgtca cgcgcgggca gacggtcggc
    25201 tacgtgggct cgacggggtt cagcaccggc ccgcacctgc acttcgaggt gcgggtcaac
    25261 ggcaaccccg tcgacccgat gggctacctc tgagccggcg cgttcgtttg ccgcccctcc
    25321 cgcgaccgac taccatctac ggttgatgcg cgcgctacgc accatcatcg tcgtccttct
    25381 cgtctcgctc gggttcctcg gcggctactt cgccggctgg tacctgcacg gggagaacac
    25441 cgtgcgcctc agtcccacgc agcagacgtc ggccaaggag gccggcgagc tgcagcagcg
    25501 catcatcgag gagctgcagg gccgctacta ccggccggtg gacgtcgaca agctctcgga
    25561 ggccggggtc aacggcatgc tcaagtcgct caaggacccc tacaccgtgt acctcacgcc
    25621 cagggaggtg caggagttcg aggagaagga gcgcggctcc tactcgggca tcggcgcctc
    25681 cctgcagaag accgaggacg gcctcgtcat cacgtcggtg ttcgacgggt cgccggcgaa
    25741 ggccgcgggc ctcgttcccg gcgaccggat cctcagcgtc gacggcaagt ccaccaagag
    25801 cgtcgccatc gagaccagca tcagccgcat caagggcgag gagggcacca ccgtcgtcct
    25861 cagcatccag tccaaggaca agacgaagcc cgccagcgac gtcagggtcg tgcgcaggac
    25921 catcgagatc cccgagacga gcaagcgcat catcgacgac aagggcacca aggtcggcta
    25981 cgtgcagctc tacgagttcg gcgggctcgc cggccgcgac gtgcgcgaga acgtggacga
    26041 gctgagcaag aagggcgcgg agtggttcat cctcgacctg cgctacaacg gcggcgggct
    26101 gctcacgcag gccgtcgacg tgaccggggt cttccagacc gggctggtca cgtccaccgc
    26161 cggcctgcac tcgcccgagg aggagctcac cgccaccggc ccggtcgcca cggacaagcc
    26221 gatggtcgtg ctcgtcaacg gcttctccgc cagcgcctcg gagatcgtga ccggcgcgct
    26281 caaggatcac gaccgcgcca ccatcatcgg caccaagacc ttcggcaagg gcctggtcca
    26341 gagcatcgtc gacctgggca acggcgccgc gctcaagctc accaccgcgg tctacctcac
    26401 gcccgacggc accaacatca acaagaaggg catcacgccc gacatcgtcg ccgcggacga
    26461 ccccaagacg aagaaggacg acgcgctgca gcgcgcgctg cagtacatcg ccgcccagga
    26521 gaagtagctc gatgagccct gcgcggcgtc cggaggagcc acgggaccgc gcccgtccgc
    26581 tgccgtaccg cgtcgcccgc tggggcaagt tctggtcgct cgaacccctc ttcgcagacc
    26641 agcgctcgta tctcgtcgcc aagggcggca cgccgcccag gctcgacgac ctcgtgctcg
    26701 ccgtgccctc gcacggcgac cgccgccgga tcgtcgaggt cctcggcggc gccgacgacg
    26761 tcaaggccgt cctgcgcgcg ctgctgcacg ccaaaggcgt gcgccagggg ttcgccgacg
    26821 ccgtgctgga ggaggcggcc gccgtcaagg ggcgcgccgc gcgccccgac cacggccgcc
    26881 gcgacctcac ggcgctgccc acgttcacca tcgaccccga caccgcgcgc gacttcgacg
    26941 acgccatctc cgtcgcccgc gagggcgagg gctaccgcgc gcacgtgcac atcgccgacg
    27001 tctcgtactt cgtcgacgac gacggcgaga tcgagcgcga ggcgcgccgc cgcacctcca
    27061 gcctctacct gccgctgttc gcggagccca tgctgccggc ggcgctcagc agcgacctct
    27121 gcagcctggt gccccggcag ccgcgcaagt gcgtcaccgt cgagttcacc ttcgacgccg
    27181 ggggccggcg gacgagcacg cagtactacc gctcgctcat cagcagcgac caccggctca
    27241 cctacgggtt cgccgacacg gtgatcgggc ctgccggcgt ggcggccggc gagggggtcg
    27301 acgaccccgc cggcgcgctc gcggccgcgg cgccgctcgc cgaccccaag gccgccgtgg
    27361 ccgccctgcc cggcgcgcac ctcgtcgccg gccgggcgga gccgccgcgc gacgtgcccg
    27421 acgcgaccat ggcggcggac gacgcgttga gcgcgcagct gctgctggcc gccgagctcg
    27481 ccggggtgct gcgccgccgg cgcctcgccc gcggggcgct caccatcggc tcgttcgagc
    27541 ccgagtacgc gttcgaccac gcgggcgagc tgagcggagc cgccgcgcgc ccggagacgc
    27601 cctcgcacgc gctggtcgag gagttcatgc tcgccgccaa cgaggccgtg gccgagtacc
    27661 tgctgcgcaa gaaggcgcgc acgctgtacc gcgtgcacga acccccggag ccggcgagca
    27721 cgcgcgagct gctcgaccag ctggaggaac tcggcgtgcc cacgccggcg ttccccagcg
    27781 gcccggcggt gcccgccgcc cagctccagg aggcgtacgg gcggctcagc cgcctcgtcg
    27841 gcgaggcgag cgtccgcgag aagcgcggcc ggctcgcctg gtccacgctc atcctgcgct
    27901 cgctcaaaca ggcgcggtac gggccggcca acctcgggca cttcggcctc gccagcccgg
    27961 cgtacctgca cttcacctcg ccgatccgtc gctacccgga cctcgtcacg catcgcgccc
    28021 tgctgttcca ccttggtgaa gcaggctccg agctgggaga cgccgaactg gcgaccgcgg
    28081 cggacgactg ctccacgcgc gagcgcgact tctcgcgcct ggaactcacg ggcgacgacg
    28141 tcgcgctgtg cttcctcctc gagcgcaggc tcgaggcgga gggctgggag cagatgttca
    28201 ccggcgagat cgtagggttg gtgggcggtg ggctgttcct gcgcttcggc gaggtgttcg
    28261 agggattcct ctcgtcacgc cgcctgggcg gcgagcgctt cgaggtgagc gagcacgaga
    28321 cggcgctcgt gggcgaggcc accgggatcc gttaccggct cggcgacccg gtggacgtca
    28381 aggtcgagcg cgtcgaccgg ctctccggca aggtcgacct cgcaccggcg caccctggag
    28441 gaggccgcac cgcgaccgct cccggccgtc gtccggcgcg gcggaccacg ggctcgcgcc
    28501 cgccgcgccg ccgcgccccc gggcgcgaga cgcagcgcaa gaaccgctga ggcggccgag
    28561 gcggcgctcc gcgcccggcg cggcgcctca ccgatgcggt accctaggcg ccatgacacg
    28621 tgagaccggg atcaagcgcg tcgcggagaa ccgcaaggcg cgccatgact acttcatcga
    28681 cgacaccttc gaggccggca tcgcgctggt cggcaccgag gtgaagtcgc tgcgcgaggg
    28741 gcgcatcaac ctgcgcgaca cctacgtgga ggtgcgcggg agcgccacct cgcccgagct
    28801 cttcctcgtc ggcgcccaca tcagccccta cgaccagggc aacatctgga accacgaccc
    28861 gctgcgggcg cgcaagctgc tcctgcaccg gagcgagatc gagcgcatcg ccggcaaggt
    28921 gcgcgagaag ggctacacgg tcgtcccgac caaggtgtac ttcaaggacg ggcgcgccaa
    28981 ggtggagatc ggcctggcgc gcggcaagaa gctctacgac aagcggcacg acctcgccga
    29041 ccgcgacgcc aagcgggaca tcgagcgtgc gctgcgcggt cgcggggaag acacgcggta
    29101 ggagccggcg tcatcccgtg gcgccggcct gacgagcttt gaggggggag gctcccatgg
    29161 atacgctgag ccaggagcag atcgcggcgg cgctgggcga gctcgacggc tggggtctgg
    29221 acggcgaggc gctgacgcgc acgttccgct tcgccgactt cgtgcacgcc atcggcttcg
    29281 tcgagcacct cgccgaggtg gccgaggagc agaagcacca cccggacatc gacatccgct
    29341 tcaacaaggt cac
//
LOCUS       DASUMX010000243         6270 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1236915, whole genome shotgun sequence.
ACCESSION   DASUMX010000243 DASUMX010000000
VERSION     DASUMX010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 6270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6270
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1236915"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..548
                     /locus_tag="VFZ86_11630"
                     /pseudo
     CDS             102..548
                     /locus_tag="VFZ86_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009349332.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The beta chain is a
                     regulatory subunit; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
     gene            559..990
                     /gene="atpC"
                     /locus_tag="VFZ86_11635"
     CDS             559..990
                     /gene="atpC"
                     /locus_tag="VFZ86_11635"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HEX5642971.1"
                     /translation="MSDERTLQVEVVTPDGQVYAGDVAMVVLPGVDGELGILPRHQPL
                     VTLLAIGETRIRTVTGDWDYIATGIGYAEVLFDRVLVVVDHGELAGAIDIARAEEAQE
                     RARERMRASGDLGAQAEVDYFRAEQALRRAENRLNVARRTR"
     gene            1033..2376
                     /gene="murA"
                     /locus_tag="VFZ86_11640"
     CDS             1033..2376
                     /gene="murA"
                     /locus_tag="VFZ86_11640"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009077032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEX5642972.1"
                     /translation="MDGSSEHDARLEVRGGRRLEGDVVLSGAKNSALKLLAASLLTTD
                     PCVIRRVPRITDVATMVTMLRALGADAGGQGDALRVQAGSGLTGCAPYELVRTMRASI
                     IVMGPLVARLGEATIAMPGGCNIGPRRIDFHIRGLEKLGAHIDIEHGFIRVHSDGLKG
                     AVVPLDYPSVGATENLVMAATAAEGETVIENAAREPEIMDLCAFLGGMGAEIEGAGSS
                     TIHVAGGRPLHGVDHAVIADRIEAGTYLLMGAATAGRVTTVGLDPAHLELFLAKLRAM
                     GVQVEEGDERITVSRDGPLAPVDISTLPHPGFATDLQAQTMVLLSLAEGTSIITENVF
                     ENRFVVVDELNRLGAGIETSGHHAVIAGPRTLSGTVVEAPDLRGGAALVTAALVADGV
                     TEVRGIHHIDRGYEDLSGKLRGLGADVSRAGDEPRTGPETRASLRSCDDEAARSA"
     gene            2397..4445
                     /locus_tag="VFZ86_11645"
     CDS             2397..4445
                     /locus_tag="VFZ86_11645"
                     /inference="COORDINATES: protein motif:HMM:NF015755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEX5642973.1"
                     /translation="MGSYRVYRVGEDEHVREASVAAPSPLARGGSGAGRGRETAPRRW
                     WLWALLAAAAVAAALLYWFYGREVVGNSRAALDLAELSGRVPDWVVVGAPVVVVAAVA
                     LVTAYLAFGRSLAVKLAGVAVVVLVLATPGLAVGYANGLVSDVGGTGSAARTPEEVAA
                     IEAADEEIDRPLPDKPMNILLIGSDKSTVPGDPGRSDTQMLVRLDPETKSISMLSLPR
                     DLLVEIPGYGQDKMNTAYSYGGPKLVIETFKELTGLPVNGWIEINFAGFWHVVNILGG
                     VYLPIDHKYFVPASADYKSINLEPGYQLVRGKQALNYVRYRHDQKGDFTRMQRQQLFL
                     KEAQRQSERWSGDWGRVLKLIKAITGETKSSLSSLKKIQPLVDLAFEVDTAEVYQAHL
                     EGLTPMIDGISYVTATEGEIADAVSRFTHPAQAPAKAKAERIGKKMFAVKVYNGSGID
                     GLATTAASQLSAQGYDAAAVADDYGYPGTVTGVYAPETLESQAKAIGAMLSPSEVTVV
                     PRAQGTSEGITVFVASSFDGTIEIPEEAVQEQQTLLKDQKVEWATWQGFDAETPLRLQ
                     APTAWASGFTYDQFRDYSIETTEGKTSKAFVAVVRTAQSGYWSIQAMRWLDPPAIRDP
                     SARQKIGGRTYMLFYRGDRLHMVAWKERGTLYWVLNTLDDQLSNDLMMGLARSCKPVK
                     "
     gene            4442..6100
                     /locus_tag="VFZ86_11650"
     CDS             4442..6100
                     /locus_tag="VFZ86_11650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642974.1"
                     /translation="MNRLRAVARRLDPWAALLLGGLLAMGVVILLATPGPWQRVVGEE
                     APVRLEAPAPDDVALFVMAGRGGSCTGIVWLHLDAEKKALAAVVVAPRISGFSPTDGY
                     APVAAIAGSAGPSAAAAALGGALGVSIDAWIALDRKAMDLAVEAMVPMNEVRAARTRF
                     REARAAWRGRGGEANAWATQYESLRISLPQVPFEELGVVAFSNYVLGFGFVRSDLTLQ
                     GATSLAEALKEVDRDLVEVRAAPVIVERSRGGEVWHADASRVEPLRQSLALGIRPPET
                     GRLITERARVARVLVVAPLARQAADRYAGEVRRRLAASAGAPVEVTLVSGDDERLAFR
                     AARELDRRPALAVLVAPAEPGARATVAVTKVCALLRDRRQEAVVSGPLPASVGEPSSA
                     AADREALAAAVSAGRLPVSWPSPPSRDGEGDGGRGLIAAARSNVQTLVRACWPGTLAP
                     RLASTRLGFAYVAARHTGVGVRSPSSTVTERLLRRLRLWGYPGTRLAPDAAVPDQVGG
                     ATLIAFRPGSREAALALAGDLGLPPGSVFRADDAPRKLVLADGN"
BASE COUNT          929 a         2213 c         2295 g          833 t
ORIGIN      
        1 tgaggtgagc gacgagcgca cactccaggt cgaggtcgtc acgccggacg gccaggtgta
       61 cgcaggggac gtcgccatgg tggtgctccc cggcgtggac ggcgagctcg ggatcctccc
      121 gcgccatctc ggagaagggc atctacccgg ccgtcgaccc gctcgactcc aactcgcgca
      181 tcctcacgcg cgacgccgtg ggcgacgagc actacgaggt cgcgctgcgc gtccaggaga
      241 tcctgcagcg ctacaaggag ctgcaggaca tcatcgccat cctcggcatg gacgagctct
      301 ccgacgagga caaggtcatc gtgcagcgcg cccgcaagat cgagcgcttc ctcagccagc
      361 cgttcaccgt cgccgaggcg ttcacgggca cgccgggcaa gtacgtcccg ctggccgaca
      421 cggtgcgcgg cttcaaggac gtcgtcgagg gcaagtgcga cgagcttccc gagcaggcct
      481 tcttcatggt cggcggcatc gaggaggcgg tcgagaaggc caagaagctc tccggcgccg
      541 acgcgtgaga ggcgtgaggt gagcgacgag cgcacactcc aggtcgaggt cgtcacgccg
      601 gacggccagg tgtacgcagg ggacgtcgcc atggtggtgc tccccggcgt ggacggcgag
      661 ctcgggatcc tcccgcgcca ccagccgctg gtgaccttgc tggccatcgg cgagacgcgc
      721 atccgcacgg tcacgggtga ctgggactac atcgcgaccg gcatcgggta cgcggaggtc
      781 ctcttcgaca gggtgctcgt cgtcgtcgac cacggcgagc tcgccggcgc catcgacatc
      841 gcgcgcgcgg aggaggcgca ggaacgcgcc cgcgagcgca tgagggcgag cggcgacctg
      901 ggcgcgcagg ccgaggtcga ctacttccgc gccgagcagg cgctgaggcg cgccgagaac
      961 cgcctcaacg tcgccagacg cacccgctga gacgccggcc cgcggttcac cggctatact
     1021 actgagcgtc tgatggacgg ctcgagcgaa cacgacgcgc gcctcgaggt gcgcggcggc
     1081 cggcggctgg agggcgacgt cgtcctctcg ggggccaaga actccgccct caagctgctc
     1141 gccgccagcc tgctcaccac cgacccctgc gtcatccgcc gcgtcccgcg gatcaccgac
     1201 gtggccacga tggtgacgat gctgcgcgcc ctcggcgccg acgccggcgg ccagggcgac
     1261 gcgctgcgcg tgcaggcagg gtccggactc accggctgcg ctccgtacga gctggtgcgc
     1321 acgatgcgcg ccagcatcat cgtcatgggc ccgctcgtgg cgcgcctcgg ggaggccacc
     1381 atcgccatgc cgggcggctg caacatcggg ccgcggagga tcgacttcca catccgcggc
     1441 ctggagaagc tcggcgccca catcgacatc gagcacgggt tcatccgcgt gcacagcgac
     1501 gggctcaagg gcgccgtggt cccgctcgac tacccgagcg tcggcgccac cgagaacctg
     1561 gtgatggcgg cgacggccgc cgagggcgag acggtgatcg agaacgccgc ccgggagccg
     1621 gagatcatgg acctctgcgc cttcctcggc ggcatgggcg cggagatcga aggggcaggc
     1681 tcgagcacca tccacgtcgc cggcgggcgg ccgctgcacg gtgtggacca cgcggtgatc
     1741 gccgaccgca tcgaggcggg tacgtacctg ctcatgggcg ccgccaccgc aggccgggtc
     1801 acgaccgtgg gcctggaccc ggcccacctc gagctgtttc tcgccaagct gcgcgcgatg
     1861 ggcgtgcagg tggaggaggg cgatgagcgc atcacggtga gccgtgacgg cccgctcgcc
     1921 ccggtcgaca tctcgacgct cccgcacccc ggcttcgcca ccgacctgca ggcgcagaca
     1981 atggtcctgc tgtcgctcgc cgaagggacc tcgatcatca ccgagaacgt gttcgagaac
     2041 cgcttcgtcg tggtcgacga gctcaaccgg ctcggagccg gcatcgagac cagcggccac
     2101 cacgccgtga tcgccggccc gcggacgttg agcgggaccg tggtcgaggc cccggacctg
     2161 cgcggtggag cggcgctggt caccgccgca ctcgtggcgg acggcgtcac ggaggtgcgc
     2221 ggcatccacc acatcgaccg cgggtacgaa gacctgtccg gaaagttgag aggcctcggc
     2281 gccgacgtct cgcgcgcggg agacgagcca cgaaccgggc ctgagacgcg cgcgtctctc
     2341 cggtcatgcg acgacgaggc cgcacgcagc gcgtgagcgc gccgcggcga atgagtatgg
     2401 gcagctaccg ggtctacagg gtcggagaag acgagcacgt ccgggaggcg agcgtcgccg
     2461 cgccctcgcc gctggcgcgc ggcggctccg gggcaggccg cggccgggaa acggcgccgc
     2521 ggcgctggtg gctctgggcg ctcctcgccg cagcggccgt cgccgcggct ctgctctact
     2581 ggttctacgg ccgcgaggtc gtgggcaact cccgggccgc cctcgatctg gccgagctct
     2641 cgggcagggt cccggattgg gtggtggtgg gggcgccggt cgtggtggtc gccgccgtcg
     2701 ccctggtcac cgcctacctc gccttcggac gcagcctcgc ggtcaagctg gccggcgtgg
     2761 ccgtggtggt gctcgtgctg gccacgcccg gactggccgt cggctacgcc aacggcctcg
     2821 tctccgacgt cggcgggacg ggcagcgccg ccaggacccc cgaggaggtc gcggcgatcg
     2881 aggcggccga cgaggagatc gaccggccgc tccccgacaa gccgatgaac atcctgctga
     2941 tcggctcgga caagagcacg gtcccgggcg accccggccg ttcggacacc cagatgctgg
     3001 tgcgcctcga cccggagacc aagagcatct cgatgctgtc gctgccgcgc gatctgttgg
     3061 tcgagatccc cggctacggc caggacaaga tgaacacggc ctactcctac ggggggccga
     3121 agctcgtgat cgagacgttc aaggagctca ccgggctgcc cgtcaacggc tggatcgaga
     3181 tcaacttcgc cgggttctgg cacgtggtca acatcctggg cggcgtgtac ctccccatag
     3241 accacaagta cttcgtcccg gccagtgccg actacaagtc gatcaacctg gagcccgggt
     3301 accagctggt gcgcggcaag caggcgctca actacgtccg ctaccgtcac gaccagaagg
     3361 gcgacttcac gcggatgcag cgccagcagc tgttcctcaa ggaggcgcag cgtcagtcgg
     3421 agcggtggag cggcgactgg ggccgggtgc tcaagctgat caaggcgatc acgggcgaga
     3481 ccaagtcgag cctcagttct ctcaagaaga tccagccgct ggtggacctc gccttcgagg
     3541 tcgacacggc cgaggtgtac caggcgcacc tcgagggcct cacgcccatg atcgacggca
     3601 tctcctacgt gaccgcgacc gagggagaga tcgccgacgc ggtgagccgt ttcacccacc
     3661 cggcgcaggc gccggcgaag gcgaaggcgg aaaggatcgg caagaagatg ttcgccgtga
     3721 aggtctacaa cggcagcggc atcgacgggc tggccaccac cgcggcgtcg cagctgagcg
     3781 cgcagggcta cgacgcggcg gcggtggcag acgactacgg ctatccgggc acggtgacgg
     3841 gcgtgtacgc gcccgagacc ctcgagagcc aggccaaggc gatcggagcg atgctctcac
     3901 cctcggaagt gactgtcgtc ccgagggcgc aggggaccag cgagggcatc accgtgttcg
     3961 tcgcctcgtc gttcgacggc accatcgaga tccccgaaga ggccgtacag gagcagcaga
     4021 cgctgctgaa ggaccagaag gtcgagtggg cgacgtggca gggcttcgac gccgagacgc
     4081 ccctccggct gcaggcgccc acggcgtggg cgtcgggctt cacctacgac cagttccgcg
     4141 actactcgat cgagacgacg gagggcaaga cctccaaagc gttcgtcgcc gtcgtccgga
     4201 cggcgcagtc cggctactgg agcatccagg cgatgcgctg gctggacccg cccgccatcc
     4261 gggaccccag cgccaggcag aagatcggcg gccgcaccta catgctcttc taccggggtg
     4321 accggctgca catggtcgcc tggaaggagc gcgggacgct gtactgggtg ctcaacacgc
     4381 tcgacgacca gctctccaac gacctcatga tgggcctcgc gaggtcctgc aagcccgtga
     4441 agtgaaccgg ctgcgcgcag tggcgcggcg gctcgaccct tgggcagcgc tcctgctcgg
     4501 cggcctcctc gccatggggg tcgtcatcct gctggccacg cccggcccgt ggcaacgcgt
     4561 ggtgggcgag gaggccccag tgcgcctgga ggcgccggcc cccgatgacg tggcgctgtt
     4621 cgtcatggcc ggccggggcg gctcctgcac cggcatcgtc tggctgcacc tcgacgcgga
     4681 gaagaaggcg ctcgcggcgg tcgtggtcgc gccgcgcatc tccgggttct cgccgacgga
     4741 cgggtacgcg ccggtcgcgg cgatcgccgg cagcgccggg ccgagcgccg ccgccgcggc
     4801 gctcgggggc gcgctcggcg tgagcatcga cgcgtggatc gccctcgacc gcaaggcgat
     4861 ggacctcgct gtggaggcca tggtcccgat gaacgaggtc cgcgccgcgc ggacgcggtt
     4921 tcgcgaggcc cgcgcggcgt ggcggggacg cggcggtgag gcgaacgcgt gggcgacaca
     4981 gtacgagagc ctgcgaatct ccctgcccca ggtgccgttc gaggagctcg gggtggtcgc
     5041 cttctccaac tacgtgctgg gcttcgggtt cgtgcgcagc gacctcaccc tgcagggagc
     5101 gacgtccctc gcggaggcgc tcaaggaggt cgaccgcgac ctcgtggagg tgcgcgccgc
     5161 gcccgtcatc gtggagcgtt ctcgcggcgg cgaggtgtgg catgcggacg cgagccgcgt
     5221 ggagcctctg cgccagtcgc tcgcgctggg catccgtccg cccgagacgg ggagactgat
     5281 cacggagcgc gcccgcgtcg cgcgcgtgct cgtcgtcgcg cccctcgcgc ggcaggcggc
     5341 ggaccgttac gccggcgagg tgcgccggcg tctcgccgcg tcggcagggg cgcccgtcga
     5401 ggtgaccctg gtgtccggcg acgatgagcg cctggcgttc cgcgccgcgc gcgaactcga
     5461 ccggcggccg gcgctcgcgg tgctggtcgc gccggcagag cccggtgcgc gcgcgacggt
     5521 cgcggtcacc aaggtgtgcg cgctgctgcg cgaccgccgc caggaggcgg tcgtctccgg
     5581 tccgttgccg gcgtctgtgg gcgagccgtc gtccgccgcc gcggaccgcg aggcccttgc
     5641 ggcggcggtg agcgccggac gcctgccggt ctcctggccg tcccctccga gccgggacgg
     5701 agagggtgac ggcggccggg gcctcatcgc ggccgcccgg agcaacgtcc agacgctcgt
     5761 gcgcgcctgc tggccgggga ccctcgcacc gcgcctcgcc tcgacgcgcc tcgggttcgc
     5821 ctacgtggcc gcgcgccaca ccggcgtcgg ggtcaggagc ccctcctcga cggtcaccga
     5881 gcgcctgctg cgacgcctgc gcctgtgggg ctatcccggg acgcgtctcg ctccggacgc
     5941 tgccgtcccg gaccaggtcg gcggcgccac cctcatcgcc ttccggcccg gctcacgcga
     6001 ggcggcgctg gccctcgccg gcgacctggg gctgccgcca ggaagtgtct tcagggctga
     6061 cgacgcgccg cgaaagctgg tcctggcgga cgggaactga cgcaggaacg ccgtcggcgc
     6121 cccgcccggg agggcggggc gccgacggcg cagtaccgat cgtcgtccgg ctaggcccga
     6181 ctgcgacgga gcagcgcccc cccgccgatg agcgcgaagc ccgctgcgag gatcaggaac
     6241 acctcaccac cggtgaacgc gagctcaccg
//
LOCUS       DASUMX010000244         6150 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_663692, whole genome shotgun sequence.
ACCESSION   DASUMX010000244 DASUMX010000000
VERSION     DASUMX010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 6150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6150
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663692"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2022)
                     /gene="ligD"
                     /locus_tag="VFZ86_11655"
     CDS             complement(<1..2022)
                     /gene="ligD"
                     /locus_tag="VFZ86_11655"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HEX5642975.1"
                     /translation="MAELRTYRRKRDFTKTGEPEGGSERTPEAAEERVAGGLFCIQKH
                     AATRLHHDLRLELDGVLVSWAVPKGPSLDPRNKRLAVHVEDHPVEYGDFEGTIPKGEY
                     GGGTVMLWDTGTWEPMGDPHKGLEKGELKFRLSGERLHGGWVLVHTRGAGGDEENQWL
                     LIKERDDEARPGEPDPWGADDRSVATGRTMEEIAAGTAPRERRARASGGGGAKSDGRA
                     QDARARIPASIPLSLATLVEEPPEGDGWLHEIKYDGYRIAARVDRTGVRLFSRNQLDW
                     TARFSEVAEALEALATDGAWLDGEVVVLDARGVSDFGRLQRFVKEGQPGDLTYIAFDL
                     LFEDGEDLRALPLLERKRRLEHLLRRGGQAVHGVVRYGDHIEGLGGDVFAEACLQGLE
                     GVVSKRAGDTYAGRRTRSWVKSKCGRRQEFVVGGFTDPSGARSGFGALLLGAYDGEGA
                     LRYAGRAGSGFDDATLTRLHARLKKLERRTPPFSDPPTGAQARGVHWTSPKLVAEVAF
                     TEWTSEGQLRHPVFKGVREDKAPEDVVREVEPRGGADAPGESPARAAADDSRGGASAR
                     RASPGADKPVVRGVRISHPDRVVFPDPGLTKLDVARYYEAVAELMVPYLERRPLTVIR
                     CPGGIGAECFFQKNVTPSVPRSVAKVRVRGADEKTVVYPVVDTPEALLAM"
     gene            complement(2029..2850)
                     /locus_tag="VFZ86_11660"
     CDS             complement(2029..2850)
                     /locus_tag="VFZ86_11660"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEX5642976.1"
                     /translation="MARPLWKGAISFGLVTIPVSVFTATRRDELRFRQLDRRDNTPIR
                     EKRVNEETGEEVPWEDVVKGFEYDEGAFVILDQDDFSKANVKASETIDIVQAVPSDAI
                     PPQYFERPLYVVPAKTGVKAYHILRETLRRTGRVAVATVVMRGRQHLAALLADEQVIT
                     LELLRFSHEIKAVSEIEAGDALEAPDVSEKEIQMAEQLVAALDEPWQPEQFRDEYRDD
                     LLALIEQKAKTGKTPVKRGGGGRQKGAEVVDMMELLKKSVGEAKGRKGQRGRKVG"
     gene            complement(2909..4186)
                     /gene="dacB"
                     /locus_tag="VFZ86_11665"
     CDS             complement(2909..4186)
                     /gene="dacB"
                     /locus_tag="VFZ86_11665"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HEX5642977.1"
                     /translation="MKAARTPRARLLVALTSLALALFLATPGVAAAGLKDRIAATLAR
                     YGMAGSGTSVAVFDLTAKRSVYQLRPDVLRLPASNEKLVTSSAALAAWTAGFRFSTQL
                     FIDAPGPDEEGVVHGDVYLRGLGDPTFSTASFQKRHYGMETGDVHDLVTGLKDLGVTR
                     VTGRVIADEGYFDSTRRVANWRPSVLAYCGPLSALTLNEGFAGDGGYVADPALAAATT
                     LTKRLRAAGVRVAHAPARGVAPVTATLACTERSAPLSRVLAAMNKPSDNFLAEELLKG
                     LGAGFGAGGTTVAGADVAERFLQSIGLTQGFRIRDGSGLSYQDKLTARVVLRILGAMA
                     RRPDFPVFRRSLAIAGVDGTLKDRMRGTAAAGNVRAKTGTLNAASSLSGYVTTANGHT
                     LSFAILMNGSPVPVARAHAAQDAVAVLLARATP"
     gene            complement(4236..4868)
                     /locus_tag="VFZ86_11670"
     CDS             complement(4236..4868)
                     /locus_tag="VFZ86_11670"
                     /inference="COORDINATES: protein motif:HMM:NF013116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HEX5642978.1"
                     /translation="MAILLDSASLDDAAAAARLGFVRGITTNPALMARETKEPLAHLA
                     RLLEAFPGGPICYQPTRSDHVAMAEQARAAAALAPDRVVAKLPATLEAITLAVELREE
                     GIDSALTAVYSPAQALLAHEAGCMWIIPYVDRAARHSAGAVVVEQAAILEQLDSPTRI
                     LAASLKTAPQVVESILDGAHDITAPLDVLRALPAHPLTESAVREFAAHWE"
     gene            5073..>6150
                     /locus_tag="VFZ86_11675"
     CDS             5073..>6150
                     /locus_tag="VFZ86_11675"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEX5642979.1"
                     /translation="MSVDVDPVRTIDTPTRLVHGPGAVSFLPEVVAELEMRRPLLVTD
                     EGVVAAGLAARVIDLLPDPVVFTEVVPNPDIDLADAAADLYVAEGCDGLVALGGGSPM
                     DVAKAVGVVAAHGGSIAQYEWGRDPIACRIPPLVTVPTTAGTGSEVTLWAVITDPGRK
                     VKFNVGGTALIAAHAAVIDPELMLGLPPAVTAATGMDALAHAVECYTCDYHQFFNDAV
                     ALEAVELVAAWLERAVADGSDLAARTGMAHAATLGGMAYGTESAGAAHAMSQSAGGVH
                     DCSHGALTARLLAPVCEYNAPASPERYARLARGLGADVAGLDPLEAALAGVRELDRLT
                     DAVGIPTLAQLGFSEDEIPLLARIA"
BASE COUNT          769 a         2196 c         2299 g          886 t
ORIGIN      
        1 catggccagc agcgcctccg gcgtgtccac cacggggtag acgaccgtct tctcgtccgc
       61 gccgcgcacg cgcaccttgg ccacggagcg cgggaccgac ggcgtcacgt tcttctggaa
      121 gaagcactcg gcgccgatcc cgccggggca gcggatgacg gtgaggggcc gccgctcgag
      181 gtagggcacc atgagctcgg cgaccgcctc gtagtagcgg gcgacgtcga gcttggtgag
      241 gccggggtcg gggaagacga cgcggtccgg gtggctgatc ctgacgccgc ggaccaccgg
      301 cttgtccgcg ccgggggagg cgcgccgcgc ggaggcgccg ccacgggagt cgtccgccgc
      361 cgccctcgcc gggctctcgc ccggggcgtc cgcgccgccg cgcggctcga cctcgcgcac
      421 cacgtcttcg ggcgccttgt cctcgcgcac gcccttgaag accgggtggc gcagctggcc
      481 ttcgctcgtc cactccgtga aggcgacctc ggccacgagc ttcggcgacg tccagtgcac
      541 cccgcgggcc tgcgcgcccg tcggcgggtc ggagaacgga ggggtgcggc gctcgagctt
      601 cttcaggcgc gcgtgcaggc gggtgagggt ggcgtcgtcg aacccgctcc cggcgcgccc
      661 ggcgtagcgg agcgcgccct ccccgtcgta cgcgccgagc aggagggcgc cgaagccgct
      721 gcgcgcgccg gacggatccg tgaagccgcc gaccacgaac tcctggcggc ggccgcactt
      781 gctcttgacc cacgagcgtg tgcgccggcc ggcgtacgtg tcgccggcgc gcttggagac
      841 cacgccctcg aggccctgca ggcaggcctc ggcgaagacg tcgccgccca gaccctcgat
      901 gtggtcgccg tagcgcacca cgccgtgcac ggcctggccg ccgcgccgga gcaggtgctc
      961 cagccggcgc ttgcgctcca gcagcggcag cgcgcgcaga tcctcgccgt cctcgaacag
     1021 caggtcgaag gcgatgtagg tgaggtcccc cggctgcccc tccttgacga accgctgcag
     1081 gcggccgaag tcgctgacgc cgcgcgcgtc gaggaccacg acctcgccgt ccagccaggc
     1141 gccgtcggtg gcgagcgctt cgagagcctc ggcgacctcg gagaaccgcg ccgtccagtc
     1201 cagctggttg cgcgagaaca ggcgcacgcc ggtccggtcc acgcgggcgg cgatgcggta
     1261 gccgtcgtac ttgatctcgt gcagccagcc gtcgccctcc ggcggctcct cgaccagcgt
     1321 cgcgaggctg agggggatgg aggcggggat acgggcgcgg gcatcctgcg cgcggccgtc
     1381 acttttcgcg ccgccgccgc cgctcgcccg ggcgcggcgc tcgcgcggcg ccgtgccggc
     1441 ggcgatctcc tccatggtgc ggccggtcgc gacgctgcgg tcgtccgcgc cccacgggtc
     1501 gggctcgccc ggccgcgcct cgtcgtcgcg ctccttgatg aggagccact ggttctcctc
     1561 gtcgccgccg gcgccgcgcg tgtgcaccag cacccagccg ccgtgcaggc gttcgccgga
     1621 gaggcggaac ttgagctcgc ccttctcgag gcccttgtgc gggtcgccca tcggttccca
     1681 ggtgcccgta tcccagagca tcaccgtgcc gccgccgtac tcacccttgg ggatcgtgcc
     1741 ctcgaagtcg ccgtactcga cggggtggtc ctcgacgtgc acggcgaggc gcttgttcct
     1801 ggggtcgagg ctcggccctt tagggacggc ccaactcacg aggacgccgt cgagctccag
     1861 ccgcaggtcg tggtgcagac gggtggcggc gtgcttctgg atgcagaaca ggccgccggc
     1921 tacgcgctcc tcggcggcct ccggtgtccg ctcgctcccg ccctccggct cgccggtctt
     1981 cgtgaagtcg cgcttgcgcc ggtaggtgcg cagctcggcc attcgggctc agcccacctt
     2041 gcggccgcgc tggcccttgc gccccttggc ctcacctacg ctcttcttga ggagctccat
     2101 catgtcgacg acctcggcgc ccttctgccg gccgccgccg ccgcgcttga ccggcgtctt
     2161 gccggtcttg gccttctgct cgatcagggc gagcaggtcg tcgcggtact cgtcgcggaa
     2221 ctgctccggc tgccacggct catcgagcgc cgcgacgagc tgctcggcca tctggatctc
     2281 cttctcggac acgtcgggcg cctcgagcgc gtcgccggcc tcgatttcgc tcaccgcctt
     2341 gatctcgtgg gagaagcgca gcagttccag ggtgatgacc tgctcgtcgg cgaggagcgc
     2401 ggccaggtgc tggcggccgc gcatcacgac ggtcgcgacg gcgaccctgc cggtgcgccg
     2461 cagggtctcg cgcaggatgt ggtacgcctt gacgccggtc ttggccggca ccacgtacag
     2521 cggccgctcg aagtactgcg gggggatcgc gtcgctgggg accgcctgca cgatgtcgat
     2581 cgtctcgctg gccttgacgt tggccttgct gaagtcgtcc tggtcgagga tcacgaaggc
     2641 gccctcgtcg tactcgaacc ccttgacgac gtcctcccac ggcacctctt cgccggtctc
     2701 ctcgttcacg cgcttctcgc ggatgggcgt gttgtcgcgc cggtcgagct ggcggaagcg
     2761 caactcgtcg cgccgggtcg cggtgaacac cgacaccggg atggtgacga ggccgaagct
     2821 gatagcgccc ttccagagtg gacgtgccat gcactcaggg tgcccagcga ggccgcccgg
     2881 gtaacgcgtc gtgcgtggag gcggcccgtc agggggtggc gcgcgcgagg agcacggcga
     2941 cggcgtcctg ggccgcgtgc gcccgcgcga ccggcacggg gctgccgttc atcaggatcg
     3001 cgaagctgag ggtgtggccg ttggccgtgg tcacgtaccc ggagaggctg ctcgcggcgt
     3061 tgagcgtccc cgtcttggcg cggacgttgc cggcggcggc ggtgccgcgc atgcggtcct
     3121 tgagcgtgcc gtccacaccg gcgatcgcca gggagcggcg gaagacgggg aagtccgggc
     3181 gcctggccat ggcgccgagg atcctgagga ccacgcgcgc ggtgagcttg tcctggtagc
     3241 tgaggccgga gccgtcgcgg atgcggaagc cttgagtcag gccgatggac tgcaggaagc
     3301 gctcggcgac gtcggcgccg gccaccgtgg tgccgccggc gccgaacccc gctccgagcc
     3361 ccttgagcag ctcctcggcg aggaagttgt cgctcggttt gttcatcgcc gcgagcacgc
     3421 ggctcagcgg cgccgagcgc tccgtgcagg cgagcgtcgc ggtgacgggc gcgaccccgc
     3481 gggcgggcgc gtgcgcgacg cgcacgccgg cggcgcggag ccgtttggtg agtgtggtcg
     3541 cggcggcgag cgcaggatcc gcgacgtagc cgccgtcgcc ggcgaagccc tcgttgagcg
     3601 tcagggccga gagcggcccg cagtaggcga ggacgctcgg ccgccagttg gcgacgcgcc
     3661 gcgtgctgtc gaagtagccc tcgtcggcga tcacgcggcc ggtgacgcgc gtcacgccga
     3721 gatccttgag gccggtgacc aggtcgtgga cgtcgccggt ctccatgccg tagtgccgct
     3781 tctggaagga cgcggtggag aaggtggggt cgccgaggcc gcgcaggtag acgtcgccgt
     3841 gcacgacgcc ctcctcgtcg ggtcccgggg cgtcgatgaa gagctgggtg ctgaagcgga
     3901 agccggcggt ccaggcggcg agcgccgccg aggacgtgac gagcttctcg ttggaggccg
     3961 gcaggcgcag cacgtccggc cgcagctggt agacggagcg cttcgcggtg aggtcgaaga
     4021 cggcgaccga ggtgcccgag ccggccatcc cgtagcgggc cagggtcgcc gcgatgcggt
     4081 ccttgaggcc ggccgccgcg acgccgggcg tggcgaggaa gagcgcgagg gccaggctcg
     4141 tcagggcgac gagcagcctg gcccggggtg tccgtgcagc cttcatcgcg gtccaccgta
     4201 gcaggacgcg gcgggcggcg cctcccgccg cgccgtcact cccagtgcgc cgcgaactcg
     4261 cgcaccgccg actccgtgag cgggtgcgcg ggcagcgcgc gcagcacgtc cagcggcgcc
     4321 gtgatgtcgt gggcgccgtc gaggatgctc tccacgacct gcggcgccgt cttgaggctg
     4381 gcggccagga tgcgcgtggg gctgtcgagt tgctccagga tcgccgcctg ttccacgacg
     4441 accgcgcccg ccgagtgccg ggcggcgcgg tccacgtacg ggatgatcca catacaaccg
     4501 gcctcgtggg cgagcagcgc ctgcgccggc gagtagacgg cggtgagggc gctgtcgatg
     4561 ccctcctctc gcagctccac cgccagggtg atcgcctcga gcgtcgccgg cagcttggcg
     4621 accacgcggt cgggcgcgag ggccgccgcg gctcgcgctt gctccgccat cgcaacgtgg
     4681 tcgctccggg tgggctgata gcagatgggg cctcccggga acgcctcgag cagccgggcg
     4741 aggtgcgcga gcggctcctt cgtctctcgc gccatgagcg ccgggttggt ggtgatgccg
     4801 cggacgaagc cgagcctggc ggccgcggcc gcgtcgtcga gagaggctga gtcgagcagg
     4861 atcgccatcg ttcactccag cgtcgagtct gtccgggaag gtgcgtgcgc accgcggcaa
     4921 ccttcccacc aagcacgctc tcgggcaagt gggaaggaac acaactctta ccgtgaagca
     4981 gagagtgaga cttctgcgcc tgggcagtgc cgcgacgcta ggatgggggc ggcacgcacc
     5041 agagacatga gacgaagagc ggaggccgca gcatgagcgt ggacgtggac cctgtgcgca
     5101 ccatcgatac gccgacgcgg ctggtgcacg gccccggggc cgtctccttc ctcccggagg
     5161 tggtcgcgga actcgagatg aggcggccgc tcctcgtgac ggacgagggc gtcgtggccg
     5221 ccggcctcgc cgcccgcgtc atcgacctgc tgccggaccc ggtcgtcttc accgaggtgg
     5281 tgcccaaccc cgacatcgac ctcgccgacg ccgccgcaga cctgtacgtg gccgagggct
     5341 gcgacggact cgtggccctg ggcggaggct cgccgatgga tgtcgccaag gcggtcggcg
     5401 tcgtggccgc ccacggcggc tcgatcgcgc agtacgagtg ggggcgcgat cccatcgcct
     5461 gccgcatccc gccgctggtg accgtgccca ccacggcggg caccggcagc gaggtcactc
     5521 tctgggcggt gatcaccgac ccgggccgca aggtgaagtt caacgtcggc ggcacggctc
     5581 tcatcgcggc gcacgccgcc gtcatcgacc ccgagctcat gctcgggctg ccgccggcgg
     5641 tcactgccgc gacgggcatg gacgcgctgg cgcacgcggt cgagtgctac acgtgcgact
     5701 accaccagtt cttcaacgac gccgtggccc tcgaggccgt cgagcttgtc gctgcgtggc
     5761 tcgaacgcgc ggtcgcggac ggctccgacc tcgcggcgcg cacggggatg gcgcacgcgg
     5821 cgaccctggg cggcatggcc tacggcaccg agagcgccgg cgccgcccac gccatgagcc
     5881 agtctgcggg cggcgtccac gactgttcgc acggcgccct cacggcacgg ctgctggcgc
     5941 cggtgtgcga gtacaacgcg ccggcgagtc ccgagcggta cgcgcgcctc gcgcgcgggc
     6001 tcggggcgga cgtcgccggc ctcgacccgc tggaggcggc gctggccggc gtccgcgagc
     6061 tcgaccggct gaccgacgcg gtcggtatcc cgacgctcgc gcagctcggc ttctccgagg
     6121 acgagatccc gctgctggcg cggatcgcct
//
LOCUS       DASUMX010000245         3511 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_807611, whole genome shotgun sequence.
ACCESSION   DASUMX010000245 DASUMX010000000
VERSION     DASUMX010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_807611"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="VFZ86_11680"
     CDS             <1..636
                     /locus_tag="VFZ86_11680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642980.1"
                     /translation="YDHRGEEGINLIRQYVEDYAAEHSGRYPLPAEVDGDGGAGSGHY
                     WPSNPWDHYDMAQRRDRGSFSYEVSADRTSYVLRLHRALKNDYVLRGAIMTSPWRHLL
                     ASLEDEILRRSGRILAGYVDQWSLQHAGALPTVAEMAPAGATGAAHPDWPQDPSGGAM
                     QPGAGPGAYSYAPGAAGAYTLTVHLHSGDFQAGGTAPSPAAPARGAGSPES"
     gene            654..1352
                     /locus_tag="VFZ86_11685"
     CDS             654..1352
                     /locus_tag="VFZ86_11685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642981.1"
                     /translation="MTRRVIVVALATAVLALASAAAAPRAEAVSFVDKQVQAGALLIQ
                     NYINTYGQAHRFVYPPKSMVKKGGGLPNATVIWPSNPWTGRIMAPGTSRGTYTYTLGA
                     GNLSYRLTVHLSSGNYVLKSAMPSWFKTERNQAARQNLLLLQRYVEAYAAAHGGSYPA
                     ADLVTSAGFGASYVWPANPWTGAGMAAGDELGSYSYAGGGASYALKVMLTSGWSASFG
                     PLPVMGQLTTQQGG"
     gene            complement(1476..2945)
                     /locus_tag="VFZ86_11690"
                     /pseudo
     CDS             complement(1476..2945)
                     /locus_tag="VFZ86_11690"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
     gene            3098..3235
                     /locus_tag="VFZ86_11695"
     CDS             3098..3235
                     /locus_tag="VFZ86_11695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642982.1"
                     /translation="MSPRWLTMLLIALLIAALTFFVSRKLGFTLLLLPLFFVWGGRPR
                     D"
     gene            3235..>3511
                     /locus_tag="VFZ86_11700"
     CDS             3235..>3511
                     /locus_tag="VFZ86_11700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642983.1"
                     /translation="MVTPPKRVSREIRSARFSRRPPSFPVTLVALAIVFALAWTFFVR
                     SGPSASAPLVQGIQGTYTWRPVDGPGEDGTFGALAGGDAGGKASVPED"
BASE COUNT          471 a         1316 c         1201 g          523 t
ORIGIN      
        1 tacgaccacc gcggcgaaga gggcatcaac ctcatccgcc agtacgtcga ggactacgcc
       61 gccgagcaca gcggccggta ccccctcccc gccgaggtcg acggcgacgg cggcgccggc
      121 agcggccact actggcccag caacccctgg gaccactacg acatggcgca gcgccgcgac
      181 cgcggctcct tctcctacga ggtctcggcg gaccggacct cgtacgtcct gcgcctgcac
      241 cgagccctga agaacgacta cgtcctgcgc ggcgccatca tgacgagccc gtggcggcac
      301 ctgctggcga gtctcgagga cgagatcctg cggcgcagcg ggcgcatcct cgccggctac
      361 gtcgaccagt ggtccctgca gcacgccggc gcgctgccca cggtcgccga gatggcgccg
      421 gccggcgcga ccggcgcggc gcaccccgac tggccgcaag accccagcgg cggcgccatg
      481 cagcccggcg caggaccagg cgcgtacagc tacgcgccgg gcgccgccgg cgcctacacc
      541 ctcaccgtcc acctccactc cggcgacttc caggccggcg gcaccgcgcc gtcgcctgcg
      601 gcgccggcgc gcggcgccgg ctctccggag tcctgactcc tcaggaggca cccgtgaccc
      661 gacgtgtgat cgtcgtcgcc ctggcgaccg cagtcctcgc cctggcatcc gccgcggcgg
      721 cgccccgcgc cgaggccgtc agcttcgtcg acaagcaggt ccaggccggc gcgctgctca
      781 tccagaacta catcaacacc tacggccagg cgcaccggtt cgtctacccg cccaagtcca
      841 tggtcaagaa gggcggcggg ctgcccaacg cgaccgtgat ctggccctcg aacccctgga
      901 ccggccggat catggccccc ggcacctcgc gcggcacgta cacctacacg ctcggcgccg
      961 ggaacctgag ctaccgcctc accgtgcacc tctccagcgg caactacgtg ctcaagagcg
     1021 ccatgccctc gtggttcaag accgagcgca accaggcggc caggcagaac ctgctgctgt
     1081 tgcagaggta cgtcgaggcc tacgccgccg cccacggcgg ttcgtacccg gccgcggacc
     1141 tggtgacgtc cgccggcttc ggcgcgagct acgtctggcc cgccaacccg tggaccggcg
     1201 cgggaatggc cgccggcgac gagctgggca gttacagcta cgccggcggc ggcgcgagct
     1261 acgcgctgaa ggtgatgctc accagcggct ggagcgcctc gttcggcccg ctgccggtga
     1321 tgggccagct gacgacgcag cagggcggct gatccgaagc tgaggggcgg gcggccggga
     1381 gggccacccg ccccgtcttc gttcgttgct cggcccggcg gcggcgaagc tgccgtcgga
     1441 actgccggtc gcgtccatcg cgccggccgc gccggtcacg tgccggcgcg ctccctgtcc
     1501 gccttgtcga cgatgccgcg gacctgcgcc agcacgtcgc cgggcagcgg ctcgggcgtg
     1561 tgcgtcgcga gcaggtctct ggccttctcc atggcgcgcg tgtacatgtc gctcgcgccg
     1621 cgtcgctccc agtcctcgcg cacgcgccgg tcgatcagct ccggctgcga cgtgtcgtgc
     1681 atgtgccgca tcgtcgcgtc gaggctgagg aagtcgccga acgagccgac cgcggcgatg
     1741 tcgtccaccg ccagcgactc gtcgtccacg cgcacgccgc cgacgacgtg cttgatcatc
     1801 cgcgccatct cgttgtcgat gacgagctgc gcgtaatcga aggtgacgcc cgactcgatc
     1861 attcccaggc cgtagatcag gttggtccct gccaacgctg ccgtgagggc cgtgagggtc
     1921 ttctcgtgac cggtctgcac gtcactgagc ttgctgtccg cctacgcacc ggcgatgtag
     1981 ctcggcaggc ggtactgcct ggcgagctgg gccgcgccgg cgctgatcag cgcgcactcc
     2041 ggcgtgccga cggaggccgc caccagacgg aggtccatgg cggtcgtcga cgagccgtac
     2101 agctgcttgc tgccgcgctc ggtgagctgg ccgaggacga tgccggcgag cacctcggcg
     2161 ttgtgcgtca ccagcgtccc tgcgagcgtc accgggctgg agccgccggc catcgccatg
     2221 ctgagcacgc agctcggcag gccggcgcgc gccgactcga tgacgatctc gcagaagtcc
     2281 ttgacgagct tgagcgggct gaccgggcag gtgcccatca ccatcatcgg acgcctgcgg
     2341 acctcgtccc tgccgccctg gacggcggcg agcatgtcca cggtgcggtg caccgcgtcg
     2401 ccgtcctggg cgccgcccga gatcggcttg gtctgattga cgatggccgc ctcgtggttg
     2461 tgcaggctga acgactccgg gggctgatcg ctggagacga ccgccatctc gaagacgtcg
     2521 acctcgggga gagcgtcgac gagacgcgcg atctcgccga ggtctctctt cgtcgacgcg
     2581 cgcagctccc cggtctgcgg gtcgatgacc ttgatgccct cgccgaagtt gctgaagccc
     2641 acgcggccgg gctcgaggca gatgtcgttc ctcgggtcgc ggccgcacag cacgaccttg
     2701 ggcggcgcgc tcgcgatcgc gtcctccacg acgtgcggcg ggatgcgcac gatgtgcgcg
     2761 tccctgtcga cgacgcaccc gccgtcgctg tagatgtcga gcgcctcgtc cagctccacc
     2821 cagacgccgg tgcgctccag cacctcgagc gtcgcgagat ggatgtcctg gatctcgtcc
     2881 tcggtgaaga cgtctaactt gaggccgcca ctccggcggc ggccggcgaa caggttgcgc
     2941 ttcacgccct gcctcctcag gctgcggatg attcgggcgg tgctacgtaa tcacagcgcg
     3001 ggcgggcggc gccaccggcc gctcgccgcg acaccgcctg cgcccgcgcc gggacgagcg
     3061 gcttgcgtcc gggtggcgcg gcgggcaggc taagtccatg tctccccgct ggctgaccat
     3121 gctgctcatt gccttgctga tcgcggcgct gaccttcttc gtctcgcgca agctcggctt
     3181 caccttgctg ttgctgcccc tgttcttcgt gtggggcggc cggccccgag actgatggtc
     3241 acgcctccga agcgggtcag ccgggagatc cgctcggcac ggttcagccg caggccgccg
     3301 agcttcccgg tcaccctcgt cgcgctcgcc atcgtcttcg ccctcgcgtg gacgttcttc
     3361 gtgcgctcgg gcccgtccgc gtccgcgccg ctggtgcagg gcatccaggg gacgtacacg
     3421 tggcgccccg tcgacggccc gggcgaggac ggcacgttcg gcgccctcgc cggcggcgac
     3481 gcgggcggga aggcgagcgt cccggaggac g
//
LOCUS       DASUMX010000246         4722 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1813763, whole genome shotgun sequence.
ACCESSION   DASUMX010000246 DASUMX010000000
VERSION     DASUMX010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4722
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1813763"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VFZ86_11705"
     CDS             <1..232
                     /locus_tag="VFZ86_11705"
                     /inference="COORDINATES: protein motif:HMM:NF015987.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEX5642984.1"
                     /translation="ERSRPFRARPGIRLDADMAAAIRKLAQLDAETRALPGRDCGVCG
                     APTCAALAEDIVMERAARALCPYVVLEEESAT"
     gene            229..582
                     /locus_tag="VFZ86_11710"
     CDS             229..582
                     /locus_tag="VFZ86_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine kinase"
                     /protein_id="HEX5642985.1"
                     /translation="MKLEDIARELGFTELTPEVAGNGETEIDRGYASDLLSDVLAHAP
                     EGGLLVTLQVHLNVIAVASHAELAAVVFAGGRKPEDDVLAKAAAEGLSLYSAPADTFD
                     IVGRLYALGVKGNHA"
     gene            579..1319
                     /locus_tag="VFZ86_11715"
     CDS             579..1319
                     /locus_tag="VFZ86_11715"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX5642986.1"
                     /translation="MNVCFADLHVHTALSPCADDEMTPPAIVAAALAEGLAMIAVCDH
                     NSAGNVAAVQEAAGARLAVLAGMEITTAEECHVVGLFPDAAAAEAAGADVGATLGPID
                     DGYEAFFGEQRLLDAAGRETGRERLALATATTLDVDRVVELVHRHGGLAVAAHIDRGS
                     FGVIGQLGFFPAEAGFDAVELSRHVPAGSERVAEFAVHGLPILHSSDAHYRADVGAAR
                     TAVTCERPGFEELALAVHGRDGRSIGDA"
     gene            1312..1806
                     /locus_tag="VFZ86_11720"
     CDS             1312..1806
                     /locus_tag="VFZ86_11720"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5642987.1"
                     /translation="MHDLSLYLLDILENSVRAGATVIATAIAADRERDELSILVEDDG
                     PGLPVEPEQALDPFFTTKANKKTGLGLSLFRQAAEAAGGGLSVGRSDELGGVSVRAWM
                     RLGDVDRPPLGDVAASVATMVVTNPEIEFRVDVSDDGARTSLRGPDIPQRFGELTAFQ
                     EAHQ"
     gene            1864..2403
                     /locus_tag="VFZ86_11725"
     CDS             1864..2403
                     /locus_tag="VFZ86_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007715801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEX5642988.1"
                     /translation="MAEAVKSCACCAEEPTEEELLERLDAVIAEYKGRPGALIPVLQL
                     AQGIFGYLPEVALKHIARELRKPYSEVAGVVGFYSFFTTVPRGKHLIRICLGTACYVR
                     GGVAVMDAFKKELGIDLGGTTEDRQFSLEIGRCFGACGLAPVIMIDDDVHHRVKPKRV
                     NEILAQYAEEPVLAETRSA"
     gene            2403..2774
                     /locus_tag="VFZ86_11730"
     CDS             2403..2774
                     /locus_tag="VFZ86_11730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HEX5642989.1"
                     /translation="MADKITSPEQLKALAEKAKADIDLREGRKETQVTVHMGTCGIAA
                     GAREIVAAFMTEINANGVSSASLHQSGCAGLCEEEPMATITLADGTLYRYGLLDKDKV
                     HTIVVNHLVGGTPVEAYLIKT"
     gene            2792..>4722
                     /gene="nuoF"
                     /locus_tag="VFZ86_11735"
     CDS             2792..>4722
                     /gene="nuoF"
                     /locus_tag="VFZ86_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEX5642990.1"
                     /translation="MADVRSHFLICTGGGCIASGALDVSEAVREELKARELAGEVDVV
                     ETGCLGPCVQGPVALVYPDGVFYQNIKVDDVPEIVEEHLLKGRVVERLVSHAPGTDRT
                     QAEMDDIPFFNRQVKIVLRNSGIIDPLNIDEYIARDGYQALAKVLTSMKPEQVVEEVL
                     ASGLRGRGGAGFPTGMKWRFAQQQDNDVKYILCNADEGDPGAFMDRSVLEGDPHSLIE
                     GMVIGAFAIGAHQGYVYVRAEYPLAVERLEHALGQAREYGLLGQDIMGSGYDFDLEIR
                     MGSGAFVCGEETALMTSIEGHRGEPRPRPPFPAVSGLWGKPSVLNNVETFANIAPILL
                     KGAEWYASTGTESSKGTKVFALGGNVQHTGLVEVPIGMQLGELIYEVGGGIPGGKAYK
                     AAQLGGPSGGCIPKEHLNVPVDYATLQELGAIMGSGGVIVMDEDTCMVDIARFFLEFT
                     QEESCGKCPPCRVGTKRMLEIVERICHGEGEEGDIERLVALGETISQTALCGLGQTAA
                     NPVLSTIRHFRHEYEAHIKDKYCEAGVCSTMFKARCSNACPASVNIPGFVSLVGEERY
                     NDALKLHRERNPLASICARVCFHPCESKCMRSSLDGSLAIRHVKRFMVEQEQEPQLPE
                     ILENAENAARKVAVVGSGPA"
BASE COUNT          756 a         1729 c         1605 g          632 t
ORIGIN      
        1 cgagcgctcg cgcccgttcc gcgcgcgccc gggcatccgc ctggacgccg acatggcggc
       61 cgccatccgc aagctggccc agctcgacgc cgagacccgg gccctgcccg gcagagactg
      121 cggcgtgtgc ggcgccccca cctgcgccgc gctggcagaa gacatcgtca tggagcgcgc
      181 cgcacgcgcg ctctgtccgt acgtcgtcct ggaggaggag agcgcgacat gaagctcgag
      241 gacatcgccc gggagctcgg gttcacggag ctcacgccgg aggtcgccgg caacggcgag
      301 accgagatcg accgcggcta cgcgtcggac ctgctcagcg acgtgctcgc gcacgccccc
      361 gagggcggcc tgctggtcac cctgcaggta cacctcaacg tgatcgccgt tgccagccac
      421 gccgagctcg ccgcggtcgt gttcgccggc ggccgcaagc ccgaggacga cgtcctcgcc
      481 aaggcggccg cggagggcct ctcgctctac tcggcgcccg ccgacacgtt cgacatcgtc
      541 ggccgcctct acgcactcgg tgtgaaaggg aaccatgcgt gaacgtctgc ttcgcagacc
      601 tgcacgtgca cacggcgctg tcgccctgcg cggacgacga gatgacgccg ccggcgatcg
      661 tcgcggcggc gctcgctgag ggcctggcga tgatcgccgt ctgcgaccac aacagcgcgg
      721 gcaacgtcgc cgcggtccag gaggccgccg gcgcgcgcct ggccgtgctc gccggcatgg
      781 agatcaccac ggcggaggag tgtcacgtgg tgggcctctt ccccgacgcc gcggccgccg
      841 aggccgccgg cgccgacgtg ggcgcgaccc tgggacccat cgacgacggc tacgaggcct
      901 tcttcggcga gcagcgcctg ctcgacgcgg cggggcggga gaccggccgc gagcggctgg
      961 cgctcgccac cgccacgacg ctcgacgtcg accgggtggt cgagctggtg caccggcacg
     1021 gcgggctcgc cgtggcggcg cacatcgacc gcgggagctt cggcgtgatc ggccagctcg
     1081 gcttcttccc cgccgaggcc ggcttcgacg cggtcgagct ctcccgccac gtgccggccg
     1141 gctcggagcg cgtcgcggag ttcgccgtgc acggcctgcc gatcctgcac tcgtccgacg
     1201 cgcactaccg cgcagacgtg ggcgcagcgc gcaccgccgt cacctgcgag cggccgggct
     1261 tcgaggagct ggcgctggcc gtccacggcc gcgacggacg gagcatcggc gatgcatgac
     1321 ctctcgctct acctcctcga catcctcgag aactcggtgc gcgccggggc caccgtgatc
     1381 gccacggcga tcgccgccga ccgcgagcgc gacgagctct cgatcctcgt cgaggacgat
     1441 gggccgggcc tgccggtgga gccggagcag gccctcgacc cgttcttcac gaccaaggcg
     1501 aacaagaaga ccggcctcgg cctgagcctg ttccgccagg cggccgaggc cgccggcggc
     1561 gggctcagcg tgggccggtc cgacgagctc ggcggcgtct ccgtgcgcgc ctggatgcgc
     1621 ctcggcgacg tcgaccgccc gccactgggc gacgtcgccg cctcggtcgc cacgatggtc
     1681 gtgaccaacc cggagatcga gttccgggtc gacgtgagcg acgacggggc gcgcacctcc
     1741 ctgcgcggcc cggacatccc gcaacgtttc ggagaactca ccgcgttcca ggaggcacac
     1801 cagtgagcca cggacactat ctgcgaactg tgatcgcagc ggactcaagc gaaggagagg
     1861 ggaatggctg aagcagtgaa gtcgtgcgcg tgctgcgccg aggagccgac cgaggaggag
     1921 ctcctcgaac gcctcgacgc ggtcatcgcc gaatacaagg gcaggccggg cgccctgatc
     1981 cccgtgctgc agctggcgca gggcatcttc ggctacctgc ctgaggtcgc cctcaagcac
     2041 atcgcgcgcg agctgcgcaa gccgtacagc gaggtcgccg gtgtggtggg cttctactcg
     2101 ttcttcacca cggtcccgcg cggcaagcac ctcatccgca tctgcctcgg cacggcctgc
     2161 tacgtgcgcg gcggcgtggc cgtgatggac gcgttcaaga aggagctcgg gatcgacctc
     2221 ggcggcacca ccgaggaccg ccagttctcg ctcgagatcg gccgctgctt cggcgcctgc
     2281 ggcctcgccc cggtcatcat gatcgacgac gacgtccacc accgcgtcaa gccgaagcgc
     2341 gtgaacgaga tcctcgccca gtacgccgag gaacccgtac tcgccgagac aaggagcgcc
     2401 tgatggccga caagatcacc agccccgagc agctcaaggc gctggccgag aaggccaagg
     2461 ccgacatcga cctgcgcgag ggccgcaagg agacgcaggt gacggtgcac atgggcacct
     2521 gcggcatcgc cgccggcgcg cgtgagatcg tcgccgcctt catgaccgag atcaacgcca
     2581 acggcgtctc ctcggcgagc ctgcaccagt ccggctgcgc cggcctctgc gaagaggagc
     2641 cgatggccac catcaccctg gccgacggga ccctctaccg ctacggcctg ctcgacaagg
     2701 acaaggtgca cacgatcgtc gtcaaccacc tcgtcggggg cacccccgtc gaggcgtacc
     2761 tcatcaagac ctgagagcga gtgatccgac tatggctgac gtgagaagtc acttcctgat
     2821 ctgcaccggc ggcggctgca tcgcctccgg cgccctcgac gtgagcgagg cggtgcgcga
     2881 ggagctgaag gcgcgcgagc tcgccggcga ggtcgacgtc gtcgagaccg gctgcctcgg
     2941 cccctgcgtg cagggccccg tggcgctcgt ctatcccgac ggcgtcttct accagaacat
     3001 caaggtcgac gacgtgcccg agatcgtcga ggaacacctg ctcaaggggc gcgtggtcga
     3061 gcgcctcgtg agccacgcgc ccggcaccga ccggacgcag gcggagatgg acgacatccc
     3121 gttcttcaac cgccaggtca agatcgtgct gcgcaactcc ggcatcatcg acccgctcaa
     3181 catcgacgag tacatcgccc gcgacggcta ccaggcgctc gccaaggtgc tcacctcgat
     3241 gaagcccgag caggtggtcg aagaggtcct cgcctccggc ctgcgcggcc gcggcggcgc
     3301 cggcttcccc accgggatga agtggcgctt cgcccagcag caggacaacg acgtcaagta
     3361 catcctctgc aacgccgacg agggcgaccc cggcgcgttc atggaccgct cggtgctcga
     3421 gggcgacccg cactcgctca tcgagggcat ggtcatcggc gccttcgcca tcggcgcgca
     3481 ccagggctac gtctacgtgc gcgccgagta ccccctcgcc gtcgagcgcc tcgagcacgc
     3541 gctcgggcag gcgcgcgagt acggcctgct cggacaggac atcatgggct cgggctacga
     3601 cttcgacctc gagatccgca tgggctccgg cgccttcgtc tgcggcgagg agacggcgct
     3661 catgacctcg atcgaggggc accgcggcga gccgcgcccg cgcccgccgt tcccggccgt
     3721 cagcggcctc tggggcaagc ccagcgtgct caacaacgtc gagaccttcg ccaacatcgc
     3781 gccgatcctc ctcaagggcg ccgagtggta cgcgagcacc ggcaccgaga gcagcaaggg
     3841 caccaaggtg ttcgccctcg gcggcaacgt gcagcacacc ggcctcgtgg aggtcccgat
     3901 cggcatgcag ctcggcgagc tcatctacga ggtcggcggc ggcatcccgg gcggcaaggc
     3961 gtacaaggcc gcccagctcg gcggtccctc gggcggctgc atccccaagg agcacctcaa
     4021 cgtgcccgtg gactacgcca ccctgcagga gctcggcgcc atcatgggct ccggcggcgt
     4081 catcgtcatg gacgaggaca cctgcatggt cgacatcgcc cgcttcttcc tcgagttcac
     4141 gcaggaggag agctgcggca agtgcccgcc gtgccgcgtc ggcaccaagc ggatgctcga
     4201 gatcgtcgag cgcatctgcc acggtgaggg cgaggagggc gacatcgagc gtctcgtcgc
     4261 cctcggcgag accatcagcc agaccgcgct ctgcggcctc ggccagacgg cggcgaaccc
     4321 ggtgctcagc accatccgcc acttccgcca cgagtacgag gcccacatca aggacaagta
     4381 ctgcgaggcc ggcgtctgct cgacgatgtt caaggcgcgc tgctccaacg cgtgcccggc
     4441 cagcgtcaac atccccggct tcgtgagcct ggtgggcgag gagcgctaca acgacgcgct
     4501 caagctgcac cgcgagcgca acccgctggc gagcatctgc gcccgcgtct gcttccatcc
     4561 ctgcgagagc aagtgcatgc gctcgagcct cgacggctcc ctcgcgatcc gtcacgtcaa
     4621 gcgcttcatg gtcgagcagg agcaggagcc gcagctgccg gagatcctgg agaacgccga
     4681 gaacgccgcg cgcaaggtcg cggtcgtcgg ctccggcccc gc
//
LOCUS       DASUMX010000247         3851 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1814157, whole genome shotgun sequence.
ACCESSION   DASUMX010000247 DASUMX010000000
VERSION     DASUMX010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3851
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1814157"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..954)
                     /locus_tag="VFZ86_11740"
     CDS             complement(127..954)
                     /locus_tag="VFZ86_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015540514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX5642991.1"
                     /translation="MGKVLVIDLNICNGCYNCQVACKDEHVGNDWSPIAKPQPDTGQF
                     WNKVTDLERGQVPKVKVTYLHSICQHCDDAPCIPACNVEAIYRRDDGAVIIDPDRCRG
                     NQLCLEACPYEDVIWFNDSLNIAQKCTFCAHLLDDGWTEPRCVDACPTGAFRFGDEED
                     PEIQALVARAEPLKPELHVKPRVFYIGLPKPFIAGAVFEPDVDECTEGARVTAQKAGN
                     GTVYEAVTDSYGDFWLRDVEPGEYTVLVEKEGYLPQKMGPVDATRDINVGDIAVWKA"
     gene            complement(954..3551)
                     /locus_tag="VFZ86_11745"
     CDS             complement(954..3551)
                     /locus_tag="VFZ86_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015942856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX5642992.1"
                     /translation="MAEDMSKTGASGEKTLIRDISFCGVPENGSNASMVDVKDGKIVR
                     IRPMHYDWKYKKADLNPWKFEVRGSTFEPSMKTLIPPYSIAYKKRVYSPARIRYPMMR
                     VDFDPNGERNPQNRGVSKYKRISWDEALDVITSEMQRIKEKYGPTALLYQCDQHGENK
                     VVQACHGAGRKLLRAWGGFTLQIRQPDSWEGWWWGNKHFWGCEPVGQGQQSNLLWDIS
                     KNAELLLFWGCDQETTPWGWQGQLPSRLSYWWSEIGIKQIYVCPDLNYAAAVHADRWI
                     PVLPNTDAALYLAITHRWFSKGTYDKEYLATHAVGVDKYEAYVMGEEDGVEKSPEWAE
                     PICGVKARVIKALADEWASKRTTVVIGNGGPGIRGPYATEPARLQGICLAMQGLGKPG
                     QNQAKMIEWGLFDKPDQYAQPKPLRVPYLRKAYTGGHPDESNHPSFIPKTYIPKAILE
                     GECDWWGCESETADRVNQFEHYKYPADGCSRIHMVWTDSPSWITCWNSGNDYVKAMRH
                     PDIEFMFAQHPWLENDCLMADVILPVNTKLEEDDIAGDIFSGQTNLLFPEHKCIEPLG
                     ESFSDYEIVVMLAERLGLKEEYTGGKTIPELIKYGWETSRCEDLISWEELNEKGYYAV
                     PTDDGWEDIPAGFYNFYKDPEKYPLTTPTGKLEFEASGLVENFPDDLERPPVPKWVAQ
                     GVTHEETVGTERSKKYPLLVCSNHPRWSVHSQHDDITWLREIKTCKVTGPDGYQYHPI
                     WLHPSEAEKRGIKDGDVVSIFNDRGTVLAGAYITERIMPGVIYIDHGAKWDPIVPGEI
                     DRGGAINTIVPRGTTSKNAVGHAVSGFLADCEKTDMEELKAKYPEAFEKPFHPCAGPG
                     LEACIWGRA"
BASE COUNT          513 a         1244 c         1346 g          748 t
ORIGIN      
        1 gcggggccgc ccgaacggct gatgatggct cttgcccagg gatgaaggag gggccgggcc
       61 tcgcggcccg gcccctccgg ccggctctgt tcaagcgccg tccggcgcct tgtcgtcagg
      121 ccccgctcac gccttccaga ccgcgatgtc gccgacgttg atgtccctgg tggcgtcgac
      181 ggggcccatc ttctgcggca ggtagccttc cttctcgacg agcaccgtgt actcgcctgg
      241 ctcgacgtcc ctgagccaga agtcgccgta gctgtcggtg acggcttcgt agaccgtgcc
      301 attgccggcc ttctgcgccg tgacccttgc accctcggtg cactcgtcca cgtccggctc
      361 gaacacggcg ccggcgatga acggcttggg cagcccgatg tagaacacgc gtggcttgac
      421 gtgcagctcg ggcttgaggg gctcggccct ggcgacgagc gcctggatct ccgggtcctc
      481 ctcgtcgccg aaccggaacg ccccggtcgg gcaggcgtcc acgcagcgcg gctcggtcca
      541 gccgtcgtcc aggaggtgcg cgcagaaggt gcacttctgg gcgatgttca gtgagtcgtt
      601 gaaccagatg acgtcctcgt aggggcacgc ctcgaggcac agctggttgc cgcggcagcg
      661 gtccgggtcg atgatcaccg ccccgtcgtc gcggcggtag atcgcctcga cgttgcaggc
      721 cgggatgcac ggcgcgtcgt cgcagtgctg gcagatgctg tgcaggtagg tgaccttgac
      781 tttcggcacc tggccgcgct cgaggtcggt gaccttgttc cagaactggc cggtgtcggg
      841 ctggggcttg gcgatgggcg accagtcgtt gccgacgtgc tcgtccttgc aggccacctg
      901 gcagttgtag cagccgttgc agatgttgag atcgatgacg aggaccttgc ccatcacgcc
      961 ctcccccaga tgcaggcttc caagccgggc ccggcacagg ggtggaacgg cttctcgaac
     1021 gcttccggat acttggcctt cagctcttcc atgtcggtct tctcgcagtc ggcgaggaac
     1081 ccgctcacgg cgtggccgac ggcgttcttg gaggtggtgc cgcggggcac gatggtgttg
     1141 atggcgccgc cgcggtcgat ctcgccgggg acgatcgggt cccacttggc gccgtggtcg
     1201 atgtagatca cgcccggcat gatgcgctcg gtgatgtacg cgccggcgag cacggtgccg
     1261 cggtcgttga agatgctgac cacgtcaccg tccttgatgc cgcgcttctc ggcctcggac
     1321 gggtgcagcc agatcgggtg gtactggtag ccgtcgggac cggtcacctt gcaggtcttg
     1381 atctcgcgca gccaggtgat gtcgtcgtgc tggctgtgga cgctccagcg cgggtggttg
     1441 gagcacacga gcagcggata cttcttcgag cgctccgtac ccacggtctc ctcgtgcgtg
     1501 acgccctggg cgacccactt gggcaccggc ggccgctcga ggtcgtccgg gaagttctcg
     1561 acgaggccgg acgcctcgaa ctcgagcttg ccggtcggcg tggtgagcgg gtacttctcc
     1621 gggtccttgt agaagttgta gaagccggcg gggatgtcct cccagccgtc gtcggtcggc
     1681 accgcgtagt agcccttctc gttgagctcc tcccagctga tgaggtcctc gcacctcgag
     1741 gtctcccagc cgtacttgat gagctcgggg atggtctttc cgccggtgta ctcctccttg
     1801 aggccgaggc gctcggcgag catgacgacg atctcgtagt cgctgaagct ctcgccgagc
     1861 ggctcgatgc acttgtgctc ggggaagagc aggttggtct ggccgctgaa gatgtcgccg
     1921 gcgatgtcgt cctcttcgag cttggtgttc acgggcagga tgacgtcggc catcaggcag
     1981 tcgttctcga gccacgggtg ctgcgcgaac atgaactcga tgtccgggtg gcgcatggcc
     2041 ttgacgtagt cgttgcccga gttccagcag gtgatccagc tcggcgagtc ggtccacacc
     2101 atgtggatcc tggagcagcc gtcggccggg tacttgtagt gctcgaactg gttgacgcgg
     2161 tcggcggtct cggactcgca gccccaccag tcgcactcgc cctcgaggat cgccttcggg
     2221 atgtacgtct tggggatgaa cgacgggtgg ttgctctcgt ccgggtggcc gccggtgtag
     2281 gccttgcgca ggtacggcac gcgcagcggc ttgggctggg cgtactggtc gggcttgtcg
     2341 aacaggcccc actcgatcat cttggcctgg ttctggcccg gcttgcccag gccctgcatg
     2401 gcgaggcaga tgccctgcag gcgggccggc tccgtggcgt aggggccgcg gatgccgggg
     2461 ccgccgttgc cgatgacgac cgtcgtacgc ttgctcgccc actcgtcggc gagggccttg
     2521 atgacgcgcg ccttgacgcc gcagatgggc tccgcccact ccggcgactt ctcgacgccg
     2581 tcctcctcgc ccatgacgta ggcctcgtac ttgtcgacgc cgacggcgtg cgtggcgagg
     2641 tactccttgt cgtaggtgcc cttgctgaac cagcggtgcg tgatcgccag gtagagcgcg
     2701 gcgtccgtgt tggggagcac cgggatccag cggtcggcgt gcacggcggc ggcgtagttg
     2761 aggtcggggc acacgtagat ctgcttgatg ccgatctcac tccaccagta gctcagccgg
     2821 ctcgggagct ggccctgcca gccccacggg gtggtctcct ggtcgcagcc ccagaacagc
     2881 agcagctcgg cgttcttgga gatgtcccac agcaggttgc tctgctggcc ctggccgacg
     2941 ggctcgcagc cccagaagtg cttgttgccc caccaccagc cttcccagct gtcgggctgg
     3001 cggatctgga gggtgaagcc gccccaggcg cgcagcagct tgcgccccgc gccgtgacag
     3061 gcctgcacga ccttgttctc gccgtgctgg tcgcactggt agagcagggc ggtcgggccg
     3121 tacttctcct tgatgcgctg catctcgctg gtgatgacgt ccagcgcctc gtcccaggag
     3181 atgcgcttgt acttgctcac gccgcggttc tgcgggttgc gctcgccgtt ggggtcgaag
     3241 tcgacgcgca tcatcgggta gcggatgcgg gcgggggagt agacgcgctt cttgtaggcg
     3301 atgctgtacg gcgggatcag ggtcttcatg ctgggctcga acgtgctgcc gcgcacctcg
     3361 aacttccagg ggttgagatc cgccttcttg tacttccagt cgtagtgcat cgggcggatg
     3421 cggacgatct tgccgtcctt gacgtccacc atcgacgcgt tggaaccgtt ctccgggacg
     3481 ccgcagaaac tgatgtctct gatgagcgtc ttctcgccgg aggcgccggt cttgctcatg
     3541 tcctccgcca tacccttacc tccccatggt cgctgacgcg gacccgaggc gttggccggc
     3601 gcgcgggggt ccgacaggtc gtccgcaatg cggattagct cgaccgcatc acgggcatcg
     3661 tccatccggc cgggggcatt gtcaagcgat gtccgccgtg gtccggacgc ctcagtacac
     3721 ccgcagcgcg tccaggcgca cggcgaggaa gcgcgccagg gcggcggcgt cctcgaggcc
     3781 gaagcggtgc gcgtgctcga ggccggcgtc ggtgaccagg gcgatggcct cgccggggcc
     3841 gcagagcggc t
//
LOCUS       DASUMX010000248         5908 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1382866, whole genome shotgun sequence.
ACCESSION   DASUMX010000248 DASUMX010000000
VERSION     DASUMX010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 5908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5908
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1382866"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="VFZ86_11750"
     CDS             complement(<1..421)
                     /locus_tag="VFZ86_11750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642993.1"
                     /translation="MNGMVLVVLVLAAVMAAIVAVSLVVLHRVGGSAERRADELRDEV
                     EAAGDEWVVPLAGAVYQGGGSPHTRSRGRGVLGLTGRRLVFLPIAGERVSVPRVRVTG
                     VRAEERRRDAAAAHRRRLVLTLDDGEEMSFLVDDAAAW"
     gene            complement(483..557)
                     /locus_tag="VFZ86_11755"
     tRNA            complement(483..557)
                     /locus_tag="VFZ86_11755"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(523..525),aa:Asn,seq:gtt)
     gene            complement(664..1758)
                     /gene="rpoD"
                     /locus_tag="VFZ86_11760"
     CDS             complement(664..1758)
                     /gene="rpoD"
                     /locus_tag="VFZ86_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEX5642994.1"
                     /translation="MLSTVEEVRVLLEEGKEQGYLAAEHILEVLADVDLTPEQIDDVY
                     VAFHDLGIDVVGADGVHHTHGGQAAGGDQEPEAEAETKLDLSIKTASNDPVRMYLREI
                     GKVPLLTAEQEVSLAKRIERGDMDAKNALIQANLRLVVSIARRYANRGLTLLDLIQEG
                     NLGLIRAVEKFDYRRGFKFSTYATWWIRQAVSRALADQARTIRIPVHMVETINKLIRV
                     QRQLLQDLGREPTPEEIATDMGLTPDKVREIQKISQEPVSLENPVGDEGDSQLGDFIE
                     DEQSVAPSDAVGELMQGEDLGRVLELLTSRERRVLEMRYGLTDGRPHTLEEVGVQFGV
                     TRERIRQIEAKTLAKLRAYREARSLRDFVE"
     gene            complement(1911..3032)
                     /gene="rpoD"
                     /locus_tag="VFZ86_11765"
     CDS             complement(1911..3032)
                     /gene="rpoD"
                     /locus_tag="VFZ86_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEX5642995.1"
                     /translation="MTDAAEERNKPVDSGDLGIEEVRLLVDEGREQGYLASDHIADAL
                     QDVELTPDQIDGIYNLFADLGIDILEGEGAGAEVGPEAKPEDDVVPKLDLSVKTPTND
                     PVRMYLKEIGKVPLLTAEEEVSLARRIERRDMDAKRKLIEANLRLVVSIAKRYVGRGM
                     LFLDLIQEGNLGLIRAVEKFDYRKGYKFSTYATWWIRQAITRAIADQARTIRIPVHMV
                     ETINKLIRVQRQLLQDIGREPTPEEIAAEMGTTPQKVREILKISQEPVSLETPIGEEE
                     DSQLGDFIEDEDATMPVEAVSEIMQKEELNAVLGTLTHRERKVIELRFGLKGEHPRTL
                     EEVGQTFGVTRERIRQIEAKTLAKLKAYRDSQRLRDFLD"
     gene            complement(3044..4873)
                     /gene="dnaG"
                     /locus_tag="VFZ86_11770"
     CDS             complement(3044..4873)
                     /gene="dnaG"
                     /locus_tag="VFZ86_11770"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEX5642996.1"
                     /translation="MARIAQSSIEQVVAAADMVEVVGQYTDLKKAGANFSGRCPFHEE
                     RTPSFSVNPAEKLYYCFGCGVGGNLIGFVMAKENMDFPEAVEYLADKYGIALEFEESS
                     ARGDADRRRRERLRTLLEDATRYYERVLWDSPAATQARAYLAARGLADEVCRAFRVGY
                     SLPGWERLRDGALAKGFGEQDLLDAGLVIPGKRGRPYDRFRGRIMFPLGDERGRTLGF
                     GARTLGDEKPKYLNSPETPLYHKSEALFGLDKAKAAAHQEDRVYVVEGYTDVLALAQA
                     GVANVVASMGTALTEQQLGRLSRLTRNVYLCYDADAAGIGAMQRALTLGRRMNLSLHV
                     VRIPDGLDPADYVLAGAGGDGFRALAADAQTLLQFHVRLALATHDLDKPDGRARAFAR
                     LREVLSETATPMERDEELRYVADKLQLSAESVRYLLSGGGRPATGAGSGGTKGTLERS
                     GASAVGSGGAHELEVRFLAGCLAQPGPGREALAGVDESFFASAETRAALRRVKARLVA
                     GADGNTPPSADDQAAADEGGEAFAEVIVRAGRERFTATVVEELFLRLQEAQVSRLVAR
                     LKVTMKADDSERQGEELARLEGVRRSLREALRALPVDDDTEVR"
     gene            complement(4875..5336)
                     /locus_tag="VFZ86_11775"
     CDS             complement(4875..5336)
                     /locus_tag="VFZ86_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5642997.1"
                     /translation="MTVAMHRRTALIVLVSAGLIVLACLPAVVTFGLGWLQLPRIQPW
                     GAPPAKDTTSLEAWTLFAVFYLAWMLGLTVLLVWSFDRIGHHWRYYERSPLPPKKTRR
                     RARATMQAMNAEQQATVQAMRRRELREAQRRRDRERAEAMRRGPDGGGGAR"
     gene            complement(5333..>5908)
                     /locus_tag="VFZ86_11780"
     CDS             complement(5333..>5908)
                     /locus_tag="VFZ86_11780"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX5642998.1"
                     /translation="AVRRGCSVARLGREIDVQLADGRALVVRDARKRDARGVTAMLDA
                     VAAEPAATLVLRPGEALVVDWRRRIATTLRSPSGLFLVAESGGSVVGNLGLWPDGNPA
                     SAHVAWIGMSVAAGYRSAGAGGALLEAAAEWAQAAGFRRVVLGVFPENVRAMAFYERH
                     GFVREGLRREQYRRGDQYHDEVLMARSLTDR"
BASE COUNT          798 a         2120 c         2060 g          930 t
ORIGIN      
        1 gccacgcggc ggcgtcgtcg acgaggaagc tcatctcctc gccgtcgtcc agcgtcagca
       61 ccagccggcg ccggtgcgcg gcggccgcgt cgcgccggcg ctcctccgcc ctgaccccgg
      121 tgacgcgcac gcgcggcacg ctcacccgct ccccggcgat cggcaggaac acgaggcgcc
      181 ggccggtgag gcccagcacg ccgcgccccc ggctgcgcgt gtggggggag cctccgccct
      241 ggtagaccgc gccggcgagc ggcaccaccc actcatcgcc ggcggcctcc acctcgtcgc
      301 gcagctcgtc ggcgcggcgc tcggcgctgc cgccgacgcg gtgcagcacc acgagcgaga
      361 cggcgacgat ggcggccatc acggccgcga gtacgaggac cacgaggacc atgccgttca
      421 cggctcctcc aaggcagaag gggcgccgcg gccctggtgg accacggcgc cccgctgatg
      481 actggctcct cgggtaggac tcgaacctac aacccttcgg ttaacagccg aatgctctac
      541 cattgagcta ccgaggatcg ctccggccga cagagtatag gcgatgcccc gccggcgcga
      601 caaacggcag ccgtccgcgc cccggcgcag gcggggcgct cgcgcgcccg gccgtgatga
      661 gcctcactcc acgaagtcgc gcaggctccg cgcctcgcga tacgcgcgga gcttggccag
      721 cgtcttggcc tcgatctgac ggatgcgctc acgggtgacg ccgaactgga cgccgacctc
      781 ctccagcgtg tgcgggcggc cgtcggtgag gccgtagcgc atctcgagca cccggcgctc
      841 gcgcgacgtg agcagctcga gcacgcggcc gaggtcctcg ccctgcatga gctcgccgac
      901 ggcgtcgctc ggggcgaccg actgctcgtc ctcgatgaag tcgccaagct gcgaatcgcc
      961 ctcgtcgccg acggggttct cgaggctcac cggctcctgg ctgatcttct ggatctcgcg
     1021 caccttgtcg ggcgtgaggc ccatgtcggt ggcgatctcc tcgggcgtgg gctcgcggcc
     1081 aaggtcctgg agcagctgcc gctgcacgcg gatgagcttg ttgatggtct cgaccatgtg
     1141 caccgggatg cggatggtgc gtgcctggtc ggcgagcgcc cggctcacgg cctggcggat
     1201 ccaccaggtg gcgtaggtgc tgaacttgaa cccccgccgg tagtcgaact tctccacggc
     1261 gcggatgagc ccgaggttgc cctcctggat caggtcgagc aacgtgagcc cgcggttcgc
     1321 gtagcggcgg gcgatggaga cgacgaggcg caggttggcc tggatgagcg cgttcttggc
     1381 gtccatgtcg ccgcgctcga tgcgcttcgc cagcgagacc tcctgctcgg ccgtgagcag
     1441 cgggaccttg ccgatctcgc gcaggtacat gcgcaccggg tcgttgctgg ccgtcttgat
     1501 ggagaggtcg agcttggtct cggcctccgc ctcgggctcc tggtcgccgc cggccgcctg
     1561 gccgccgtgc gtgtggtgga cgccgtcggc cccgaccacg tcgatgccga ggtcgtggaa
     1621 ggccacgtag acgtcgtcga tctgctccgg cgtgaggtcg acgtcggcga ggacctcgag
     1681 gatgtgctcg gccgcgaggt agccctgctc cttgccctct tcgagcagga cgcggacttc
     1741 ctcgaccgtc gagaggatcg actcgtcagg cggtcgatct gctgctgact ctgtcactga
     1801 ctgcttcagc tccttgcttg cagcggcgga gggaacgggg ctggccgttc tggccacccc
     1861 gctcccggct ccgctgagtc gtgttcgttg tccggtcccg caaacctcgg ctagtcgagg
     1921 aagtcgcgca gccgctgcga gtcgcggtac gccttgagct tggccagcgt cttggcctcg
     1981 atctggcgga tgcgctcgcg tgtcacgccg aacgtctgac cgacctcctc caaggtcctc
     2041 gggtgctctc ccttgagtcc gaaacgcagc tcgatgacct tacgttcgcg gtgcgtgagc
     2101 gtgccgagta ccgcgttcag ctcttccttc tgcatgatct cgctcacggc ctcgaccggc
     2161 atggtcgcgt cttcgtcctc gatgaaatcg ccgagctggg aatcctcttc ttccccgatc
     2221 ggcgtctcca gactcaccgg ttcctggctg atcttcagga tctcgcgcac cttctgagga
     2281 gttgtaccca tctcggccgc gatctcctcc ggcgtgggct cacggccgat gtcctggagc
     2341 agctgtcgct gcacgcggat gagcttgttg atggtctcga ccatgtgcac cgggatgcgg
     2401 atggtgcgcg cctggtcggc gatggcccgc gtgatcgcct ggcggatcca ccaggtggcg
     2461 taggtgctga acttgtatcc cttgcgatag tcgaacttct cgacggcgcg gatgaggccg
     2521 agattgccct cctggatgag gtcgaggaac agcatgccgc gccccacgta gcgcttggcg
     2581 atggagacca ccaggcgcag gttggcctcg atgagcttgc gcttggcgtc catgtcgcgg
     2641 cgctcgatcc gcctcgccag cgacacttct tcttctgcgg tgagcagggg caccttgccg
     2701 atctccttga ggtacatgcg caccgggtcg ttggtcggcg tcttgaccga gaggtcgagc
     2761 ttggggacga cgtcgtcctc gggcttggcc tcggggccca cctccgcgcc ggcgccctcg
     2821 ccctcgagga tgtcgatgcc gaggtcggcg aagaggttgt agatgccgtc gatctggtcc
     2881 ggcgtgagct ccacgtcctg cagggcgtcg gcgatgtggt cgctcgcgag gtagccctgc
     2941 tcgcgcccct cgtcgaccag cagacggacc tcctcgatgc ccaggtcgcc actgtcgacc
     3001 ggcttgttgc gttcttcggc cgcgtcggtc acgtcagtcg ccctcaccgc acctcggtgt
     3061 cgtcgtcgac gggaagagcg cggagcgcct ctcgcaggct gcggcgcacg ccctcgagtc
     3121 tcgcaagctc ctcaccctgc ctctcactgt cgtcagcctt catcgtgacc ttgagccgcg
     3181 ccacgagccg gctcacctgc gcctcctgca ggcgcaggaa cagctcctcc acgacggtcg
     3241 cggtgaaccg ctcccgcccc gcgcgcacga tgacctccgc gaaggcctcc ccgccctcgt
     3301 ccgcggccgc ctggtcgtcg gccgatggcg gggtgttccc gtcggcgccg gccaccaacc
     3361 gcgccttgac gcggcgcagc gcggcgcgcg tctccgcgct ggcgaagaag ctctcgtcga
     3421 cgcccgcgag cgcctcgcgg ccgggaccgg gctgcgcgag gcagccggcc aggaacctga
     3481 cctcgagctc gtgggcgccg ccggaaccga ccgcggacgc gccgctccgc tcgagggtgc
     3541 ccttcgtgcc gccggagccg gcgcccgtcg ccgggcgacc gccgccggag agcagatagc
     3601 gcacgctctc cgcggagagc tgcagcttgt cggcgacgta ccgcagctcc tcgtcgcgct
     3661 ccatcggcgt cgccgtctcg gagaggacct cccgcagtcg cgcgaacgcg cgtgcccgtc
     3721 cgtcgggctt gtcgaggtcg tgagtggcga gcgccaagcg gacgtggaat tgtagcagcg
     3781 tctgcgcgtc ggccgcaagc gcccgaaaac cgtcgccgcc ggcccccgca agcacgtagt
     3841 cggcagggtc gagaccgtcg gggatgcgca ccacgtgcag ggaaaggttc atcctgcggc
     3901 cgagcgtcag cgcgcgctgc atggcgccga tgccggcagc gtcggcgtcg tagcagaggt
     3961 agacgttgcg ggtgaggcgt gagagacggc ccagctgttg ctcggtgagg gccgtcccca
     4021 tgctcgccac cacattggcg acgccggcct gagcgagggc caggacgtcg gtgtagccct
     4081 cgaccacgta cacgcggtcc tcctggtgcg cggcggcctt ggctttgtcg aggccgaaca
     4141 gcgcctcgct cttgtggtag agcggcgtct ccggcgagtt gaggtacttg ggcttctcgt
     4201 cgccgagcgt gcgggcgccg aagccgagcg tgcgtccgcg ctcatccccg agggggaaca
     4261 tgatgcggcc gcggaagcgg tcgtaggggc ggccgcgctt gcccgggatc accagaccgg
     4321 cgtcgaggag gtcctgctcg ccgaacccct tggcgagcgc gccgtcgcgc aggcgctccc
     4381 accccggcag gctgtagccc acgcgaaagg cgcggcagac ctcgtccgcg agaccgcgcg
     4441 ccgccaggta cgcccgcgcc tgcgtcgccg ccggagagtc ccacaggacg cgctcgtagt
     4501 acctggtcgc gtcctcgagc agcgtgcgca ggcgctcgcg gcgccggcgg tccgcgtcgc
     4561 cgcgagcgct cgactcctcg aactcgagcg cgatcccgta cttgtcggcg aggtactcga
     4621 cggcctcggg gaagtccatg ttctccttgg ccatgacgaa cccgatgagg ttgcccccga
     4681 cgccgcagcc gaagcagtag tagagcttct ccgccggatt gacggagaac gaaggcgtgc
     4741 gctcctcgtg gaaggggcag cgcccgctga agttcgcccc ggccttcttg aggtcggtgt
     4801 actggccgac gacctcgacc atgtcggccg cggccacgac ctgctcgatg ctgctctggg
     4861 cgatgcgggc cacgtcagcg agcccctccg ccgccgtccg gaccacggcg catcgcctcg
     4921 gcccgctcgc ggtcgcgccg ccgctgcgcc tcgcgcagct cgcggcggcg catcgcctgg
     4981 accgtcgcct gctgctcggc gttcatggcc tgcatcgtcg cccgagcccg gcgccgcgtc
     5041 ttcttcggcg gcagcggcga gcgctcgtag taccgccagt ggtggccgat gcggtcgaac
     5101 gaccacacca gcagcaccgt gagccccagc atccaggcga ggtagaacac ggcgaacagc
     5161 gtccaggcct cgagggaggt cgtgtctttg gcgggcggcg ccccccaggg ctggatacgg
     5221 ggcagctgca gccagcccag gccgaaggtg acgacggccg ggaggcaggc gagcacgatg
     5281 aggccggcac tgaccagcac gatgagcgcc gtgcggcggt gcatcgcgac cgtcaccggt
     5341 ctgtgaggga acgagccatc agcacctcgt catgatactg gtcgccccgg cggtactgct
     5401 cgcggcgcag tccctcccgt acgaacccgt gacgctcgta gaaggccatg gcgcgcacgt
     5461 tctcggggaa cacgccgagc accacgcgac ggaagccggc ggcctgcgcc cactccgccg
     5521 ccgcctcgag gagcgcgccg ccggcgccgg cgctgcggta gccggcggcg accgacatgc
     5581 cgatccaggc gacgtgcgcc gaggccgggt tcccatcggg ccacagcccg aggttgccca
     5641 cgacggagcc gccggactcg gcgacgagga agaggccgct gggagagcgc agggtcgtcg
     5701 cgatgcgccg gcgccagtcg acgacgaggg cttcgcccgg acgaaggacc agcgtggccg
     5761 ccggctccgc ggcgacggcg tcgagcatcg ccgtgacgcc gcgcgcgtcc cgcttgcgcg
     5821 cgtcgcgcac cacgagcgct cgcccgtcgg cgagctgaac gtcgatctct cgtccgagcc
     5881 tggccacaga gcaccccctt cggacggc
//
LOCUS       DASUMX010000249         4175 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_233022, whole genome shotgun sequence.
ACCESSION   DASUMX010000249 DASUMX010000000
VERSION     DASUMX010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4175
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233022"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(181..885)
                     /locus_tag="VFZ86_11785"
     CDS             complement(181..885)
                     /locus_tag="VFZ86_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020510453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5642999.1"
                     /translation="MIETPAGATRVLVVDDEPSITDLVSMALRYEQFDVRVAGNGREA
                     LDAVESFSPDLAVLDIMMPGIDGFEVARRLRNRGAQLPILFLTARDATEDKVRGLTLG
                     GDDYMTKPFSVEELVARIRAILRRVQPAAASPGRLAFADLELDEDTHEVWRAGREIEL
                     TATEFKLLRYLLLNARRVLSKDQILDHVWNYDFGGNANIVETYVSYLRRKVDAEGPPL
                     LHTVRGVGYSLRLPKD"
     gene            1074..2531
                     /locus_tag="VFZ86_11790"
     CDS             1074..2531
                     /locus_tag="VFZ86_11790"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5643000.1"
                     /translation="MFFTYVFRELRRRHRQALLTALGLAVGVALVVAVTAYADGVGRA
                     QDQVLQSLYGVGTDITVSQQRKMDEGGAQRFGMQPPDDSQAGERFSRSRVMTSPGQQP
                     LSTGKLARIAALDGVGDATGSIAMTVMNVSGEFAQVMQQGGGEGVAAAAGAQPSSGSS
                     QQDQAQMAPIDIVSFSVTGVDTADLELGPLSSAQVASGRSLTSADADARVMLVTKAYA
                     RQNSLSVGDTKRIDGRKFAVVGVVALPAGSTSSDLYIPLSWAQKLSDNEGKVNQIYVR
                     ADSADDIAAVKEAIEQVLPKATVTTSEDLAGQVSGSLSSASTLADRLGTWLAVAALIA
                     AFAVASLLTVSAVSRRVREFGTLKALGWKGRRIVGQVLGESVAVGVVGAVLGVALGVL
                     GAWLIAAFSPALEATMGNALQGAAQGGPPGMQQAADAIGGAAQSVTVSLTAPVSPQLV
                     GIAVALALAGALIAGAFGGWRASRLRPADALRRVD"
     gene            2594..3328
                     /locus_tag="VFZ86_11795"
     CDS             2594..3328
                     /locus_tag="VFZ86_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018565080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5643001.1"
                     /translation="MLYGLTGVTKRYTQRGKQIDALAGIDLAIDEGEFLAIQGSTGSG
                     KTTLLQMLGAMDRPSSGTLDFDGRDLAEMREGDLSELRSHAFGFIFQGFNLIPTLTAQ
                     ENVETALVPWRTPAGERRRRAGDALAGVGLADRARHIPSELSGGEQQRVAIARALVRA
                     PRVILADEPTGNLDERTRDDIIGLLEQLWRERGQTLIVVTHDTWVASRASRRIRLDQG
                     RVEGEDAGAGADLDSAFLAEPAVAGH"
     gene            complement(3360..4064)
                     /locus_tag="VFZ86_11800"
     CDS             complement(3360..4064)
                     /locus_tag="VFZ86_11800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643002.1"
                     /translation="MDELATGIHRWTAAHPEWRPRAEEVGSYALVANEALLLVDPLLP
                     ADADDRSAPLLAQLDRMAAAAVRVEVLVTIPYHTRSAEALYERFAAERPTRLWGHAHV
                     RKRLRAGTPLEVVPMEASGSVADIAGGAAEAYTIGRPRRSEHPMYVPSLRAVVFGDAV
                     VGAHGGLRFWNMSSGTGEHWYREVFAPTLAPLAERPIDHVLVTHGPPVIGDGQRQLAL
                     CLASPPVSAYWSPSRE"
BASE COUNT          579 a         1502 c         1520 g          574 t
ORIGIN      
        1 cgcagcgaga agtacgtgat gaggccggcc acgaccaggc cggccgcgac gcagcccagg
       61 acggccacca gcaggcgggt gcgcaggctc acggtgtcgc cttcagtccg tccgccgctg
      121 ggtccctgcg ggccggcggc cgctcaatgg tgctcgctct gcttcgcatc gcttcgggca
      181 ttagtccttg ggcaggcgca ggctgtagcc gacgccgcgc acggtgtgga ggagcggcgg
      241 gccctcggcg tccaccttgc gccgcaggta gctgacgtag gtctcgacga tgttggcgtt
      301 gccgccgaag tcgtagttcc acacgtggtc gaggatctgg tccttgctga gcacgcggcg
      361 cgcgttgagg agcaggtagc ggagcagctt gaactcggtg gccgtgagct cgatctcgcg
      421 gcccgcgcgc cagacctcgt gcgtgtcctc gtcgagctcg aggtcggcga aggcgagccg
      481 gcccggcgag gcggcggccg gctgcacgcg cctgaggatc gcgcggatgc gcgcgacgag
      541 ctcctcgacg ctgaacggct tggtcatgta gtcgtcgccg ccgagcgtga ggccgcggac
      601 cttgtcctcg gtcgcgtcgc gcgccgtgag gaagaggatc gggagctgcg cgccccggtt
      661 gcgcaggcgg cgcgccacct cgaaaccgtc gatgcccggc atcatgatgt cgagcacggc
      721 gaggtcgggc gagaagctct cgaccgcgtc gagcgcctcg cgcccgttgc cggcgacgcg
      781 cacgtcgaac tgctcgtagc ggagcgccat cgagacgagg tcggtgatgc tgggctcgtc
      841 gtcgacgacc agcaccctgg tggctccggc cggtgtctcg atcatgctga ccctccgctc
      901 cgcgcgttcc ctcgggcttc tcgcggagag tgtagccccc ttttctgaga atcgcctgtg
      961 agttcgctga acccaccatt cacaggcacc gctcaggcgc ggctcagcga cctcccaagc
     1021 ccgcgacgta caaccgggcc tgtgcaagga agccaggcgg acaaggaggc ggcatgttct
     1081 tcacctacgt gttccgggag ctgcgccggc ggcaccgcca ggcgctgctc accgccctcg
     1141 ggctcgccgt cggcgtggcg ctcgtggtcg ccgtgaccgc gtacgccgac ggcgtgggca
     1201 gggcgcagga ccaggtgctg cagtcgctct acggtgtcgg caccgacatc accgtgagcc
     1261 agcagcggaa gatggacgag ggcggcgcgc agcgcttcgg catgcagccg ccggacgact
     1321 cccaggcggg ggagaggttc agccgctcgc gggtgatgac cagcccgggc cagcagcccc
     1381 tctcgaccgg gaagctcgcc cggatcgccg ctctcgacgg cgtcggcgac gccaccggca
     1441 gcatcgcgat gaccgtcatg aacgtctcgg gggagttcgc gcaggtcatg cagcagggcg
     1501 ggggagaggg agtcgcggct gcggccggcg cgcagccgtc gtccggctcg tcccagcagg
     1561 accaggcgca gatggcgccg atcgacatcg tctcgttctc ggtcaccggc gtcgacaccg
     1621 ccgacctcga gctcgggccc ctgagctcgg ctcaggtcgc gtcggggcgc tcgctgacga
     1681 gcgccgacgc ggacgccagg gtgatgctcg tgaccaaggc ctacgccagg cagaactcac
     1741 tcagcgtcgg cgacaccaag aggatcgacg gcaggaagtt cgcggtcgtc ggcgtcgtcg
     1801 cgctgcccgc cggcagcacc tcgtccgatc tctacatccc cctgagctgg gcgcagaagc
     1861 tcagcgacaa cgagggcaag gtcaaccaga tctacgtcag ggccgacagc gccgacgaca
     1921 tcgccgccgt caaggaggcg atcgagcagg tcctgcccaa ggcgaccgtg acgacgtcgg
     1981 aggacctcgc cggccaggtg agcggatccc tctccagcgc ctcgacgctc gccgaccggc
     2041 tcggtacctg gctcgcggtc gcggcgctga tcgccgcctt cgccgtcgcc agcctgctca
     2101 ccgtgtccgc cgtgtcccgc cgcgtgcgcg agttcgggac gctcaaggcg ctcggatgga
     2161 agggccggcg gatcgtcggc caggtgctcg gcgagtccgt cgcggtgggc gtggtcgggg
     2221 cagtgctggg ggtggcgctc ggcgtcctcg gcgcctggct gatcgccgcc ttctccccgg
     2281 cgctcgaggc gacgatgggc aacgccctgc agggcgccgc gcaaggtggg ccgcccggca
     2341 tgcagcaggc ggcggacgcc atcggcggcg ccgcccagag cgtcaccgtg agcctcacgg
     2401 cgccggtgag cccgcagctg gtcggcatcg ccgtggccct ggccctggcc ggcgccctga
     2461 tcgccggcgc cttcggcggc tggcgagcct cgaggctccg tcccgcggat gcgctccgca
     2521 gagtggattg atcgccgtgg gctcatcaac gacgggtgtg ccggaggcgc acccgtcact
     2581 gagaggagtg acgatgctct acggactcac cggagtgacc aagcgctaca cccagcgcgg
     2641 caagcagatc gacgcgctgg ccgggatcga tctggccatc gacgagggcg agttcctcgc
     2701 catccaggga tcgaccggat ccgggaagac gaccctgctg cagatgctgg gcgccatgga
     2761 ccggccttcg agcggcacgc tcgacttcga cggccgcgac ctggcggaga tgcgcgaggg
     2821 cgacctctcg gagctcagga gccacgcgtt cggattcatc ttccaggggt tcaacctcat
     2881 ccccacgctg acggcgcagg agaacgtgga gaccgctctg gtcccgtggc gcacgcctgc
     2941 gggcgagcgc cgccgccggg ccggggacgc gctcgccggc gtcggtctcg ccgaccgggc
     3001 gcggcacatc ccctcggagc tgagcggcgg cgagcagcag cgcgtggcga tcgcgcgggc
     3061 gctcgtcagg gcgccccgcg tgatcctcgc cgacgagccc accggcaacc tcgacgagcg
     3121 cacgcgcgac gacatcatcg gcctgctcga gcagctctgg cgcgagcgcg gccagaccct
     3181 gatcgtcgtg acgcacgaca cctgggtcgc gagccgcgcc tcacggcgca tccggctcga
     3241 ccagggcagg gtcgagggcg aggacgccgg cgccggcgcg gacctcgact cggcgttcct
     3301 cgccgagcct gccgtcgccg gccactgacg cacgcggatc ctccgtccgc gtgcgcccct
     3361 cactcgcggg aagggctcca gtacgcgctc accggcggtg acgccaggca cagcgccagc
     3421 tgccgctgcc cgtcgccgat caccggcggc ccgtgggtga ccagcacgtg gtcgatgggc
     3481 cgctccgcga gcggtgcgag ggtcggcgcg aacacctcgc ggtaccagtg ctcaccggtg
     3541 ccgctgctca tgttccagaa gcgcagcccg ccgtgcgctc ccacgaccgc gtcgccgaac
     3601 accacggcgc gcagcgacgg cacgtacatg gggtgctcgc tgcgccgcgg ccggccgatg
     3661 gtgtacgcct cggccgcgcc gccggcgatg tcggccaccg acccggaagc ttccatcggg
     3721 acgacctcga gcggcgtccc ggcgcggagt cgcttgcgca cgtgggcgtg cccccagagg
     3781 cgggtcggac gctccgcggc gaagcgctcg tagagcgcct ccgcgctgcg cgtgtggtag
     3841 gggatcgtca cgagcacctc gacccggacc gcggccgcgg ccatgcggtc gagctgcgcc
     3901 agcagcggcg cgctccggtc atcggcgtcc gccgggagca gcgggtcgac gagcaggagc
     3961 gcttcgttcg ccaccagcgc gtacgacccg acctcctcgg ccctgggccg ccactccgga
     4021 tgcgcggccg tccaccggtg gatgcccgtc gccagctcgt ccatctcgcc tcctcgctcg
     4081 tctcccgtaa cgctgtcatg tcccggcggc gggcgcgaca atcccggagc cgtggacgaa
     4141 gccggcctct cgggccgggg aaggacgcgg cgggg
//
LOCUS       DASUMX010000250         2566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_378019, whole genome shotgun sequence.
ACCESSION   DASUMX010000250 DASUMX010000000
VERSION     DASUMX010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378019"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /gene="rnpB"
                     /locus_tag="VFZ86_11805"
     ncRNA           <1..195
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VFZ86_11805"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(463..1887)
                     /locus_tag="VFZ86_11810"
     CDS             complement(463..1887)
                     /locus_tag="VFZ86_11810"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HEX5643003.1"
                     /translation="MLPPKGVLIQLTSYTFLFAFLPVTLLVYWLLPRAVWRLAFLVLA
                     SWVFVGWFDWRFVPIMVGATTIDWVAGLLLAGGGTLRRRRWILAVALAANLAILGYFK
                     YRGFFLDSLEGLGSLVGVDADLPALRVLLPLGISFYTFSAMSYVIDVYRGDVTATRSF
                     LRYAAFIALFPRAIAGPILRWRDVEGDFGSLPRRLTTRLAALGLFFLACGMAKKLLVA
                     DVLLPHVDELFANAAHLHFFSGWAAALGYTLQLYFDFSGYSDMAVGVAYLLGFHLPQN
                     FDSPYKAANPSQFWRRWHMTLSFWMRDYLFIPLGGSRRGTVVTVRNLLIVFVLVGLWH
                     GAGWTFIIWGLLHGVYQSVHVVARKKGFTPRNEWLNRGLTFVAVVVAWVFFRAATLEQ
                     AGSVLKAMAGLNGLGSLYSAKVQIGYAFTLLVFAGLAWVNLAPNTWEIEVKPTWRYGI
                     LFGLLLGAAVLAMGRPAEFVYVQF"
     gene            1912..2412
                     /locus_tag="VFZ86_11815"
     CDS             1912..2412
                     /locus_tag="VFZ86_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011056674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5643004.1"
                     /translation="MRPTAAQMAAALGLQPHAEGGFYLETYRAAQTLRTPRGERPAAT
                     AILFLVTAESASRLHRLTSDELWVYQGGLPLELVTIAPGGELQRRVLGDFEGEALLPQ
                     ALVPAGSWQGARLAGGPHLPAELAWALVSCVVTPGFDFADFELGDREELLAEHPEHAA
                     LILALT"
BASE COUNT          432 a          884 c          933 g          317 t
ORIGIN      
        1 gaggtaagag ctcaccagcg gtcctggtga caggccggct cggcaagccc cgcccggtgc
       61 aagaccaaat aggggagcgt tcgagggttg ctcgcccgag gctcccgggt ttcggtcgca
      121 tgagccgtcc ggcaacgggc ggcccgagat ggatggtcac ccacgacagg atccggctta
      181 cggcttgcta cagaaaggcc cctcggtcca gctggacgag gggcctttcc aatgcgcggg
      241 gcggccgggc ggtgtccgtg gccggcggcc ggcgcggcag tcgacgccgg cacctgccca
      301 cggggatggg gtagtgcgtt tcggactagc acgtctgcac gtacgccctg atgtacaacg
      361 gtacacccgc gagtaccttg gcacgtgcca gactgaagcg acactaccta tcccgcgcgg
      421 cgccgcggcg ggcggcgcgg cgggcgccgc cactccgccg cctcagaact gcacgtagac
      481 gaattcggcg ggcctgccca tggccagcac cgccgccccc agcagcagcc cgaacaggat
      541 cccgtaacgc caggtgggct tcacctcgat ctcccacgtg ttcggcgcca gattgaccca
      601 cgcgaggccg gcgaacacga gcagcgtgaa ggcgtagccg atctgcactt tggccgagta
      661 gaggctgccg aggccgttca ggccggccat ggccttgagc acgctgccgg cctgttccag
      721 cgtcgcggcg cggaagaaca cccaggcgac gaccactgcc acgaaggtca ggccgcggtt
      781 cagccactcg ttcctcgggg tgaagccctt cttgcgcgcc actacgtgca ctgactggta
      841 gacgccgtgc agcaggcccc agatgatgaa cgtccacccg gcgccgtgcc agagcccgac
      901 gaggacgaac acgatgagca ggttgcggac cgtgacgacg gtgccgcgac gcgagccgcc
      961 caaggggatg aagaggtagt cgcgcatcca gaacgacagg gtcatgtgcc agcggcgcca
     1021 gaactggctc gggttggcgg ccttgtacgg cgagtcgaag ttctgcggga ggtggaagcc
     1081 gaggaggtag gcgacgccca cggccatgtc cgagtacccg ctgaagtcga agtagagctg
     1141 gagggtgtag ccgagggccg ccgcccaccc gctgaagaag tggaggtggg cggcgttcgc
     1201 gaagagctcg tccacgtgcg gcagcaggac gtcggcgacg agcagcttct tggccatgcc
     1261 gcaggcgagg aagaagaggc cgagcgccgc cagccgcgtc gtcaggcggc gcggcaggct
     1321 gccgaagtcc ccctccacgt cgcgccagcg caggatcggc ccggcgatag ctcgcgggaa
     1381 caacgcgatg aacgcggcgt agcggaggaa gctccgcgtg gcggtcacgt cgccgcggta
     1441 gacgtcgatc acgtagctca tcgcgctgaa cgtgtagaac gagatgccga gcggcaggag
     1501 cacgcgcagc gccggcaggt cggcgtccac acccaccaag gagccgaggc cctccaggct
     1561 gtcgaggaag aagccgcggt acttgaagta gccgaggatc gcgaggttgg cggcgagggc
     1621 gacggcgagg atccagcggc ggcggcgcag cgtcccgccg cctgccagga gcaggccggc
     1681 cacccagtcg atggtcgtgg cgccgaccat gatgggcacg aagcgccagt cgaaccaccc
     1741 gacgaagacc caggaggcga gcacgaggaa ggcgaggcgc cagaccgcgc gcggcagcag
     1801 ccagtacacc agcagcgtga ccggcaggaa ggcgaacagg aacgtgtagc tcgtcagctg
     1861 gatgaggacc cccttcggcg gcagcatcct atcagcgtgt atgctgccgg catgcgaccg
     1921 accgccgccc agatggccgc cgcgctcggg ctccagccgc acgccgaggg cggcttctac
     1981 ctcgagacgt accgcgccgc gcagacgctg cgcacgccgc gcggcgagcg cccggccgcc
     2041 acggccatcc tgttcctcgt caccgcggag tccgcgagcc gcctccaccg cctcacgagc
     2101 gacgagctct gggtgtacca gggcgggctg ccgctcgagc tggtgaccat cgcgccgggc
     2161 ggggagctgc agcggcgcgt gctcggcgat ttcgagggcg aggccctgct gccgcaggcg
     2221 ctggtgccgg ccggcagctg gcagggcgcc cgcctcgcgg gcggtccgca cctgccggcc
     2281 gagctcgcct gggcgctggt cagctgcgtc gtcacgccgg gcttcgactt cgccgacttc
     2341 gagctcggcg accgcgagga gctcctcgcc gagcaccccg agcacgcggc gctcatcctc
     2401 gcgctgacgt gacgtcgcgc ggccgacccg gaggcggccc ggcaggccgt gcatcccggc
     2461 cgggcagtcc cgtccggacc gcgtgatacg ctcgccgcga ccatgaccgg cggggccgac
     2521 gcacaccagg ggctgctcgt cagcggccgt ttcccggagc tcgagg
//
LOCUS       DASUMX010000251         3681 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_2104164, whole genome shotgun sequence.
ACCESSION   DASUMX010000251 DASUMX010000000
VERSION     DASUMX010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3681
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2104164"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /gene="manB"
                     /locus_tag="VFZ86_11820"
     CDS             <1..835
                     /gene="manB"
                     /locus_tag="VFZ86_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934644.1"
                     /note="converts mannose-6-phosphate to
                     mannose-1-phosphate; the resulting product is then
                     converted to GDP-mannose by ManC which is then used in the
                     synthesis of mannose-containing glycoconjugates that are
                     important for mediating entry into host cells; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEX5643005.1"
                     /translation="VVMDAANGVAGKLAPRVFAGTAIDAVEMYFEVDGTFPNHSPNPL
                     LEENSREIRARVVAEKADLGIAWDGDADRCFFIDADGEFVPGDFVTALLAEALLLKHP
                     GAKILYDLRASRAVPDVITANGGVPLLNRVGHAFFKQRMRREGGLFGGEVSGHYYFAD
                     NYNADSGFIPALLILELLSQKRATLAELLEPLRSKYFISGEINSTVDDVPAALRRIRE
                     RFSDGELGDLDGISVDYAQWHFNVRPSNTEPLVRLNLGADSRALMEEKRDLVLSVIRD
                     G"
     gene            843..1928
                     /locus_tag="VFZ86_11825"
     CDS             843..1928
                     /locus_tag="VFZ86_11825"
                     /inference="COORDINATES: protein motif:HMM:NF006426.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HEX5643006.1"
                     /translation="MTDLGPARAAEIDVSGMFAEVAGLPCQLREGYENARARLAGAFF
                     GTFPAIPPAEPQGVALCGMGGSAIGADLVLACLTGLSVPATVVRGYALPEWVGPEVLV
                     IVVSYSGETEEALACAAQARARGCAPVCVSSGGSLAAFAEAEGLPLVLVPGGGQPRAA
                     VGSLSMPLLATLEAAGLCAEHAADVTGAAAQLDADDAILAPASPDEQNPAKALARRLH
                     QRLAVVYGAGPTAPVARRWKGQINENAKAPAFFNELPELDHNELMGWTSLPHVTSSTV
                     ALFLHDESGEARLARRAELTACDYEALGVSTELVTARGASRLARLFSLVQLGDYVSCY
                     LALLYGVDPTPVDAIQALKASLAGDGA"
     gene            1935..2516
                     /locus_tag="VFZ86_11830"
     CDS             1935..2516
                     /locus_tag="VFZ86_11830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643007.1"
                     /translation="MPDEAVALVLTAAVPACRSAGAALFPGARQLELTRGQLRPPAPF
                     EGPLFALLAERPRGAVVGLVVPVLAAGAAGAGLPPGALVPVADHVGLEGRGPLTGRWP
                     AGVPRDFPSMTGIYQPADVRPRPGARVYSSGVVAAGVADVRRLSRFEARAVREGGWPI
                     VSDTLVPVAVVAAYYGVKLAACGVVQAPRSERE"
     gene            2527..>3681
                     /gene="ahcY"
                     /locus_tag="VFZ86_11835"
     CDS             2527..>3681
                     /gene="ahcY"
                     /locus_tag="VFZ86_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012189895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEX5643008.1"
                     /translation="MSNDYDIKDIGLAPEGVLRIEWADMHMPVLKAIRERFAQEKPLQ
                     GVRIGACLHVTTETANLMRTLVAGGADVVLCASNPLSTQDSVAAALVEEYGVRVYAIN
                     GEDEKTYYAHINACVDHRPNITMDDGADVIGVLHGERPDMAADIIGGTEETTTGVIRL
                     KALEAEGRLMFPIVAVNEANTKHMFDNRYGTGQSTLDGVIRATNILIAGSTVCVAGYG
                     WCGRGLAMRMKGHGADVIVLEVDPLRALEAVMDGFRVMPVAEAAKVAKLFVTATGDIH
                     VLRGEHFEAMQDGTIIANTGHFNVEIDIPALEKLAVGKRTVRDLVDEYTMADGRRLYL
                     LAEGRLVNLSAAEGHPAAVMDMSFANQSLSAEYMVAHHADLESKVYVVPQD"
BASE COUNT          514 a         1380 c         1277 g          510 t
ORIGIN      
        1 ggtggtcatg gacgccgcca acggcgtcgc cggcaagctg gcgccgcggg tgttcgcggg
       61 cacggcgatc gacgccgtcg agatgtactt cgaggtcgac ggcaccttcc ccaaccactc
      121 gcccaacccg ctcctcgagg agaacagccg cgagatccgc gcgcgcgtgg tggccgagaa
      181 ggccgacctg ggcatcgcct gggacggcga cgccgaccgc tgcttcttca tcgacgcgga
      241 cggcgagttc gtgcccggcg acttcgtcac cgcgctcctc gccgaggcgc tgctgctcaa
      301 gcaccccggg gcgaagatcc tctacgacct ccgcgccagc cgcgcggtgc ccgacgtgat
      361 caccgccaac ggcggcgtac ccctgctgaa ccgcgtcggg cacgccttct tcaagcagcg
      421 catgcgccgc gagggcgggc tgttcggcgg cgaggtgtcc ggccactact acttcgccga
      481 caactacaac gccgactccg gcttcatccc ggcgctgctc atcctcgagc tgctgtcgca
      541 gaagagggcg acgctggcgg agctgctcga gccgctgcgc tcgaagtact tcatcagcgg
      601 cgagatcaac tcgaccgtgg acgacgtccc ggccgccttg cgacgcatcc gcgagaggtt
      661 ctccgacggc gagctgggcg acctcgacgg gatctccgtg gactacgcgc agtggcactt
      721 caacgtgcgc ccgtccaaca cggagccgct ggtgcggctc aacctcggcg ccgactcacg
      781 ggcgctcatg gaggagaagc gcgatctcgt gctctccgtg atccgcgacg gctgaggtgg
      841 acgtgaccga cctcggaccg gcgcgggcgg cggagatcga cgtctccggc atgttcgccg
      901 aggtcgccgg gctcccgtgc cagctgcgtg aggggtacga gaacgcccgc gcccggctcg
      961 ccggcgcgtt cttcggcacg ttcccggcca tcccgccggc ggagccgcag ggcgtcgcgc
     1021 tgtgcgggat gggcggctcg gccatcggcg ccgacctcgt cctggcctgt ctcacggggc
     1081 tgtccgtgcc cgcgacggtg gtgcgagggt atgcgctgcc ggagtgggtc ggcccggagg
     1141 tgctggtgat cgtggtcagc tactccggcg agaccgagga ggcgctcgcc tgcgcagcgc
     1201 aggcgcgcgc gcgcggctgc gccccggtgt gcgtctcgtc cggcggcagc ctcgccgcct
     1261 tcgccgaggc ggagggtctg ccgctcgtcc tcgtgcccgg cggcggccag ccgcgcgccg
     1321 ccgtggggtc cctgtcgatg ccgctgctcg ccaccctgga ggctgccggc ctgtgcgcgg
     1381 agcacgccgc cgacgtcacc ggcgccgcgg cgcagctcga cgccgacgac gcgatcctcg
     1441 cccccgccag ccccgatgag cagaatcccg cgaaggccct cgcgcgccgt ctccaccagc
     1501 ggctggcggt cgtctacggc gccggtccca cggcgcccgt cgcccggcgc tggaagggcc
     1561 agatcaacga gaacgccaaa gcgccggcct tcttcaacga gctgccggag ctggaccaca
     1621 acgagctcat gggctggacg agcctgccgc acgtgacctc gtcgacggtc gccctgttcc
     1681 tgcacgacga gtccggggag gcgcgcctcg cgcgtcgcgc cgagctgacg gcctgcgact
     1741 acgaggcgct cggggtgtcc accgaactgg tcaccgcgcg cggcgcctca cggctcgcgc
     1801 ggctcttctc cctcgtgcag ctcggcgact acgtgagctg ctacctcgcg ctgctgtacg
     1861 gcgtcgaccc cacgccggtc gacgccatcc aggcgctcaa ggcgagcctc gccggcgacg
     1921 gcgcctgagc gcccgtgcca gacgaggccg tcgcgctcgt cctgaccgcc gccgtcccgg
     1981 cgtgccgctc cgccggcgcc gccctgttcc cgggcgctcg gcagctggaa ctgacgcggg
     2041 gacagctgcg cccgcccgcg ccgttcgagg ggccgctgtt cgcgctgctg gcggagcgcc
     2101 cgcgcggcgc cgtcgtcggg ctggtggtgc cggtgctcgc cgccggcgcg gcgggagcgg
     2161 gcctcccgcc gggcgcgctc gtgccggtcg ccgaccacgt cggcctcgag gggcgcggcc
     2221 cgctcaccgg ccgctggccg gccggcgtgc cgcgcgactt ccccagcatg accggcatat
     2281 atcaaccggc cgacgttcgt ccgcgtcccg gggctcgggt atactcgtcc ggcgtcgtgg
     2341 cggcgggcgt cgcggacgtc cggcgcctct cgcgcttcga ggcgcgggcg gtccgcgaag
     2401 ggggctggcc catcgtgtcc gacaccctgg tccccgtcgc cgtcgtcgcc gcgtactacg
     2461 gcgtcaagct ggccgcctgc ggagtggtgc aggcgccccg atccgaaaga gagtgaggct
     2521 ctgcgcatga gcaacgacta cgacatcaag gacatcggcc tcgcccccga gggcgtgctg
     2581 cgcatcgagt gggcggacat gcacatgccc gtgctcaagg ccatccgcga gcgcttcgca
     2641 caggagaagc cgctccaggg cgtgcgcatc ggcgcctgcc tccacgtgac caccgagacg
     2701 gccaacctca tgcgcacgct ggtcgccggc ggcgccgacg tggtgctctg cgccagcaac
     2761 cctctgagca cgcaggacag cgtcgccgcc gcgctcgtcg aggagtacgg cgtgcgcgtc
     2821 tacgccatca acggcgagga cgagaagacc tactacgcgc acatcaacgc ctgcgtcgac
     2881 caccggccca acatcaccat ggacgacggc gccgacgtca tcggcgtgct gcacggcgag
     2941 cgtcccgaca tggccgcgga catcatcggc ggcaccgagg agaccaccac cggcgtcata
     3001 cgtctcaagg cgctggaggc cgagggccgg ctcatgttcc cgatcgtggc cgtcaacgag
     3061 gccaacacca agcacatgtt cgacaaccgc tacggcaccg gccagagcac gctcgacggc
     3121 gtgatcaggg cgaccaacat cctcatcgcc ggcagcaccg tctgcgtcgc gggctatggc
     3181 tggtgcggcc gcggcctcgc catgcgcatg aagggccacg gcgccgacgt catcgtgctc
     3241 gaggtcgacc cgctgcgtgc gctcgaggcc gtcatggacg gcttccgcgt catgccggtg
     3301 gccgaggccg ccaaggtcgc caagctcttc gtcaccgcca ccggcgacat ccacgtgctg
     3361 cgcggcgagc acttcgaggc catgcaggac ggcaccatca tcgccaacac cggccacttc
     3421 aacgtggaga tcgacatccc ggcgctcgag aagctggcgg tcggcaagcg caccgtgcgc
     3481 gacctcgtcg acgagtacac catggcggac ggccgccgcc tctacctgct ggccgaaggc
     3541 cggctggtca acctctcggc cgccgagggc catccggccg ccgtcatgga catgagcttc
     3601 gccaatcagt cactcagcgc cgagtacatg gtggcgcacc acgccgacct cgagagcaag
     3661 gtctacgtgg tgccacagga c
//
LOCUS       DASUMX010000252         6943 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_379514, whole genome shotgun sequence.
ACCESSION   DASUMX010000252 DASUMX010000000
VERSION     DASUMX010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 6943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6943
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379514"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="VFZ86_11840"
     CDS             <1..639
                     /locus_tag="VFZ86_11840"
                     /inference="COORDINATES: protein motif:HMM:NF024138.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX5643009.1"
                     /translation="DEDAPDVVTVAMGSLEGLIALRHGLGQVAGCHLLDPATGDFNRS
                     YARALFPGRRLTLLTLAHRQQGLMLPPGNPRGYGALEEAVAAGARIVNRNRGSGTRVA
                     LDRLLAAAGVEGATVDGYDDEVTTHRQAARLVADGSAEVALGIYAAAKAEGLDFVPLL
                     EERYDLVTPTSVYESGILDPLLALLRSERLRRAVRALGGYATRETGRVTTLV"
     gene            complement(669..948)
                     /locus_tag="VFZ86_11845"
                     /pseudo
     CDS             complement(669..948)
                     /locus_tag="VFZ86_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010562043.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(1179..1382)
                     /locus_tag="VFZ86_11850"
     CDS             complement(1179..1382)
                     /locus_tag="VFZ86_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007931475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HEX5643010.1"
                     /translation="MALSARNQLSGAVKSVVLGTVMAEVVVDVGGQEVVSAITRQAVE
                     NLDVKAGDEVRVIIKSTEVMIEK"
     gene            complement(1483..2544)
                     /locus_tag="VFZ86_11855"
     CDS             complement(1483..2544)
                     /locus_tag="VFZ86_11855"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5643011.1"
                     /translation="MVRAGSAIVAVDRFELPERTTHVLLGPNGAGKSTFLRVLNGLER
                     ATGRIWFDGREIASGRDRRALRRATAAVFQRSHLLATSVRANVEMGLRARGVRGEEAH
                     ARALRAMELVGVEHLAERGRDKLSGGEAQRVSVARALAIEPRVLFMDEPMASLDPPTR
                     RSLLADLRSVIADLSIAVVWVTHDRDEAVAVADAVTFLSGGQVRQTGPAGDVFQRPRT
                     TEIAHFLGLELYIDGEVVLDRGEARLVLADGTSIVCGEAEPGPSVGCLYPEDVVLFRQ
                     PPEEGSTSLRNIRKGTVTSIETLGRVRQVTVACGVLTLVATVTQAAVAELKLEVGSPV
                     FAGFKASALHVLPRPPRRT"
     gene            complement(2616..3314)
                     /locus_tag="VFZ86_11860"
     CDS             complement(2616..3314)
                     /locus_tag="VFZ86_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5643012.1"
                     /translation="MGQIWEGFEEALRLLFSGDAEVYQIALRSLWVSTLALTIAAVIG
                     VPLGAALAQIRFWGRGGVVSLVNTGMGVPPVVVGLFVGLLLARNGPFGDLRLLYSNQA
                     MVIAQLVIALPIVVGLSMAAIQHLDEGLRMQIVALGAGRLRTFWLLLREARITLMAAL
                     MVAYGALLSEVGAVQVVGGNIRGQTQVLTTGIVERMRSGQQATAIALGIILMLLVLGV
                     LGGMTIIQQRSRRT"
     gene            complement(3350..4258)
                     /locus_tag="VFZ86_11865"
     CDS             complement(3350..4258)
                     /locus_tag="VFZ86_11865"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX5643013.1"
                     /translation="MFITASQRLKLLLMFLVVVFALMAAACGDDGAAATEESPAGDGA
                     YEADQLILATTTSLKDSGLLDELVLPAFNEAHPDITVKTIAVGSGEAMQMGRDGEADV
                     LLVHSPADEAAFMEEGNGTIRLPVAQNYFVIVGPADDPAGVADAADAAEAFAAIPDSG
                     TPFVSRGDDSGTHKKELSVWEAAGVTPEGDWYTSTGQGMGETLQVTSERQGYTLTDLG
                     TFLSMQDSLDLEVLSEESEDLRNIYSVIVVNQLVHPKTNAAGAEMFAQILVASDMQAQ
                     IGEFGTAEFGQPLFAPNAAALGGPDY"
     regulatory      complement(4448..4569)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            complement(4690..5409)
                     /locus_tag="VFZ86_11870"
     CDS             complement(4690..5409)
                     /locus_tag="VFZ86_11870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643014.1"
                     /translation="MLNVADVEAAVVTAAKVNTLTLNAVSITGVSISGQTITGGLIRT
                     AGSSHRVEISPQAYKDVRMPSGYPGAEYNPAALGAYYNSYDPDSPILTISGPDIGRGQ
                     ASITISGAGTINLFPTNPSTPRVRALGALRLIQLRERRERGRHLDQLPSGRVARAGVA
                     PARADLARARGAHGEAHRGRRKRLPPARPHRDVDGALRQCSGLRRLQPWRAPTAPCRA
                     AFGCRRPVVWEWSMTTCIATR"
     gene            5608..6585
                     /gene="moaA"
                     /locus_tag="VFZ86_11875"
     CDS             5608..6585
                     /gene="moaA"
                     /locus_tag="VFZ86_11875"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEX5643015.1"
                     /translation="MDGSQHAQGRVDYLRLSITDRCNLRCTYCMPPEGIKARGHGEIL
                     SYEELAVFARVAADVGITKVRITGGEPLVRTGCADLVGMLSRTSGIDDISLTTNGLLL
                     PRFADELAAAGLRRVNVSLDSLDPGRFTRITRGGRLEDTLAGLDAAFAAGFSPIKINT
                     LLLPGVEEELAAFVDLTREYDVHVRFIEFMPLDRRVAGEDLGGEGRLLPAGDVLRRLM
                     QQYVLIGHDGPYGHGPAQYWKVAGARGTIGFIAGVSEHFCETCNRLRLTADGRLRTCL
                     FSGRETDIRPLLGDPAVLRAAIETAVAGKTYDRCREALANERSMSQIGG"
     gene            6587..>6943
                     /gene="moaC"
                     /locus_tag="VFZ86_11880"
     CDS             6587..>6943
                     /gene="moaC"
                     /locus_tag="VFZ86_11880"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007067365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEX5643016.1"
                     /translation="MELSHLDERGRAAMVDVGGKDDTERVARAEAAVVMAPETLAIIM
                     DEAVPKGDVIAVARLAGIMAAKRTHELIPLCHQLNLTRVAVEIEADEALPGLHIVTEA
                     RLRGRTGVEMEALVAAS"
BASE COUNT         1070 a         2448 c         2335 g         1090 t
ORIGIN      
        1 gacgaggacg ctccggatgt ggtgaccgtc gccatgggca gcctcgaggg actcatcgcg
       61 ctgcgccatg ggctcgggca ggtggcgggg tgccacctgc tggatcccgc caccggggac
      121 ttcaaccgct cctacgcacg cgcgctcttc ccgggacgcc ggctcacgct cctgaccctc
      181 gcgcaccggc aacaggggtt gatgcttcct cccgggaacc cccggggcta cggcgcgctc
      241 gaggaggcgg tcgccgcggg agcccgcatc gtcaaccgca accgcggctc gggcacgcgg
      301 gtcgcgctgg accggctgct ggccgcggcc ggagtcgagg gtgcgacagt cgacggctac
      361 gacgacgagg tcacgactca tcgacaggcc gcgcgactgg tggccgacgg gtccgcggaa
      421 gtagccttgg gcatctacgc cgccgccaag gccgaaggac tcgatttcgt gcccctcctc
      481 gaggaacgct acgacctcgt gacgcccacg tccgtctacg agagcggcat cctcgacccg
      541 ctgctggcgt tgctgcgcag cgagcgcttg aggcgcgcag tccgcgcctt gggcggctac
      601 gcgacgcggg agaccggcag ggtcaccact ctcgtctagc cggccggtcg cccaggcggc
      661 gccggccgtc acgacaccag cttctccctg agcacctcat agggagtctt gccgccgagc
      721 gctgtgtgcg gtcgctgcag gttgtagaac tcctcccaga cggcgagctt ggcgcgcagg
      781 tcgacgtcgc cggtgtagtc cagcagctgg tggaactcga gctggtcggt gaggtgggag
      841 cgctccacct tgccgcctca ggtcagggct cgccggcttg atgaagacgt gctgcatgcc
      901 gaggtcctcg cagtgccagt ggaacttggc ctgccactcg tgcccgttgc gagcatgcgc
      961 ctgtgcctga ggagtcagcg caggagagag ggcgcagttc atgcccatcc aaatgctctt
     1021 gagaccggcc tgcgcgggac gacttgggca tggaacggcg tctcaagaca ccgccaaccg
     1081 cacgagctgc cgccatcttg ctgcccgtcg tcgagggcca ccatcacaag ggcggcaaca
     1141 gccaggccgg tgacgcgtca gagctcaacc atgcgcctct acttttcgat catgacctcg
     1201 gtggacttga tgatcaccct cacctcgtcg cctgctttga cgtcgaggtt ctcgacggct
     1261 tggcgagtga ttgcggacac tacctcctgc ccgccgacgt ccacgaccac ctcggccatc
     1321 actgttccaa gtaccacact cttcactgct ccagagagct gattgcgtgc actcaatgcc
     1381 atgccaatcc cccaaatacg ccacttcact agaccgtacc ccgactgagt acatgacaag
     1441 gcgcaggtgt gttgaatcga agccgatttc aacgtctggt catcacgtgc gccgaggcgg
     1501 acggggcaga acgtgcaggg cgctggcctt gaagcccgcg aagaccggcg aaccaacctc
     1561 gagcttcagc tctgcgacag cagcttgagt cacggtcgcg accagcgtca ggacgccaca
     1621 agcaaccgtc acctgccgta ctctgcccag agtctcgatt gaagtgaccg tccccttgcg
     1681 tatgttgcgc aggcttgtgc tgccttcctc cggtggttgg cgaaacagga ccacatcctc
     1741 tggatagagg cagccgacgc tgggtcctgg ttcggcctcg ccacatacga tgctcgtgcc
     1801 atcggcgagg accagacggg cctcgccgcg gtcgaggaca acctccccgt cgatgtacaa
     1861 ctcgaggccc aggaagtggg cgatctcggt cgtcctcggt cgctgaaaga catccccagc
     1921 cggccccgtc tgtcgcacct gcccgccgct gaggaaggtg accgcgtcgg cgaccgccac
     1981 ggcctcgtcg cggtcgtggg tgacccacac gacggctatc gacagatcgg cgatgacgga
     2041 gcgcagatct gccagcaggg agcggcgcgt gggtgggtcg agcgacgcca tcggctcgtc
     2101 catgaacagc accctgggct cgatcgccag cgcgcgcgcc acgctcactc gctgcgcctc
     2161 cccgccggag agcttgtccc ggccgcgctc cgccaggtgc tcgacgccga ccagttccat
     2221 ggcgcgcagc gcgcgggcgt gcgcctcctc gccacgcacg ccccgggcgc gcaggcccat
     2281 ctcgacgttc gcgcggacgc tcgtggccag cagatgcgaa cgttggaaga ccgcggccgt
     2341 ggccctgcgc agcgcccgcc ggtctcgccc ggaggcgatc tcgcgtccgt cgaaccagat
     2401 gcgcccggtc gcgcgttcca ggccgttgag cacccgcagg aaggtgctct tgccggcgcc
     2461 gttaggaccg agcaggacgt gcgtcgtccg ttcgggcagc tcgaaacggt cgacggcgac
     2521 gatggccgag ccagcgcgga ccacacggat ctccgatgcc gcgagcaccg gcgcctgacc
     2581 cgcgcggcct gtggcagccg tgttcccgga cgtcgtcacg tgcgtctgct gcgctgctgg
     2641 atgatcgtca tcccccccag cacccccagg accagcagca tgaggatgat gccgagcgcg
     2701 atggcggtgg cctgttgccc cgaccgcatg cgctccacga tcccggtggt gagcacctgc
     2761 gtctgacccc tgatgttgcc gcccacgacc tggacggcgc ccacctccga gaggagcgcc
     2821 ccataggcga ccatgagcgc cgccatgagc gtgatgcgtg cttctcgcag cagcagccag
     2881 aaggtgcgca ggcggccggc gccgagcgcg acgatctgca tccgcaggcc ctcgtccagg
     2941 tgctggatgg cggccatgga gaggccgacg acgatgggca gggcgatgac cagctgggcg
     3001 atcaccatcg cctggttgga atacaggagg cggaggtcgc cgaagggccc gttgcgtgcc
     3061 agcagcaggc cgacgaagag gccgaccacg accggcggca cgcccatgcc ggtattgacg
     3121 agcgagacca ctccgcccct gccccagaag cggatctggg cgagggcggc tccgagcggc
     3181 accccgatca ccgccgcgat ggtcagcgcc agcgtcgaga cccagagcga gcgcagggcg
     3241 atctggtaga cctcggcgtc accgctgaac agcaggcgca gcgcctcctc gaagccttcc
     3301 cagatctgcc ccacgtaccg tcccccgctt gtcgccgcct acaacgctat cagtagtccg
     3361 gtcctcccag cgcggcagca ttcggagcga acaacggctg tccgaactca gccgtgccga
     3421 actccccgat ctgcgcctgc atgtcggagg caaccaggat ctgcgcgaac atctcggcgc
     3481 ctgccgcgtt cgtcttgggg tgcacgagct ggttgaccac gatcacgctg tagatgttgc
     3541 gcaggtcctc gctctcctct gagaggacct cgaggtcgag cgagtcctgc atggagagga
     3601 aggtgcccag gtcggtcagc gtgtagccct ggcgctcgct cgtcacctgc agggtctcgc
     3661 ccatgccctg cccggtgctc gtgtaccagt cgccttccgg cgtcacgccg gctgcctccc
     3721 agaccgacag ctccttcttg tgcgtcccgg agtcgtcgcc gcgggagacg aacggcgtgc
     3781 cgctgtccgg gatggcggcg aaggcctccg ccgcgtcggc cgcgtcggcc accccggcgg
     3841 gatcgtccgc gggaccgacg atgacgaagt agttctgggc caccggcagc cggatggtgc
     3901 cgttgccctc ctccatgaac gccgcctcat cggccggcga gtgcacgagg agcacgtccg
     3961 cctcaccgtc gcgtcccatc tgcatggcct cgccggaccc gaccgcgatc gtcttcacgg
     4021 tgatgtccgg gtgagcctcg ttgaaggccg ggaggacgag ctcgtccagg aggcccgagt
     4081 ctttgaggct ggtcgtcgtg gcgaggatga gctggtctgc ctcgtaggct ccgtcgccgg
     4141 ccggggactc ctctgtggcg gcggcgccgt catcgccgca ggctgccgcc atcagggcga
     4201 agacaacgac aaggaacatg agcaagagtt tcaggcgctg gctggctgtg atgaacatga
     4261 ggaccctcct cctccgatgg acgttctcgg cattaaggcc ggaccgtgtt gaccgacgct
     4321 tcccttggct gcagccgtca ggtggacacg tcctggctct tcgccatcgg agtggggaga
     4381 ccgacgtccg cagctgcaga agctcgacgt gcgtccgcag ggaactgtgg cgccgggcca
     4441 tgagcccggt ccgtcggacg cctctccttt ccacactggg agacacggcg gtttcccgct
     4501 gtaccctgcc ggcatccggc cccataggca gtgcccgtag gagaccggcg ctcggagaag
     4561 ccgcaccata ctaaggaatc acttatagac ttcgcaaggg caaggagacg agcaccgaac
     4621 ccgggaccct ctttacctct caaaggtcgg tcctcgaccc gattgctcgc cgcgctcgcc
     4681 tctccaggtt cagcgggtgg cgatgcaggt cgtcatcgac cactcccaga cgacgggacg
     4741 tcgacatccg aaggctgcgc gacacggcgc cgtcggtgct cgccagggtt gtagtcggcg
     4801 tagtccggag cattggcgca gagccccatc gacgtcacgg tgaggacgag ccggcggtag
     4861 tcgctttcga cgccctcgat gcgcttctcc atgcgcgcct cgcgctcgtg ccaggtctgc
     4921 tcgagctggc gccactcccg ctcgcgcgac tcgccctgat ggcagctgat ccagatgccg
     4981 tcctcgctca cgccgctcac gcagttgtat gagccgcagc gcgcccaagg cgcggacgcg
     5041 cggcgtcgac gggttggtcg ggaacaggtt gatcgtgccg gcgccgctga tggtgatgct
     5101 ggcctgaccc cggccgatat ccgggccgct gatggtgagg atgggcgagt cggggtcgta
     5161 gctgttgtag taggcgccca gcgcggccgg gttgtactcc gcgccggggt agccgctcgg
     5221 catcctgacg tccttgtagg cctgcggcga gatctcgacc cggtgactgg acccggccgt
     5281 gcggatcagg ccacccgtga tcgtctgccc gctgatgctc acgccggtga tcgataccgc
     5341 gttgagcgtc agggtgttga ccttggcggc ggtcacgacg gcggcctcca cgtcggcgac
     5401 gttgagcatc gcggccgtga tcgcggcctt caaggcgtcg gcggtgcacc ccatcccgag
     5461 gcacgaacgg cgtcccaggt cgcccggctg agccgcgtcc tcaggcgatc gcccgcgcgg
     5521 cgtgggtcgc gccccagtcc tgtcccggca gtcgccgccc tctgctcatg tgacggaccc
     5581 gggcgcgagc ggtacacttg ccctcccatg gacggctccc agcacgcaca gggcagggtc
     5641 gattacctgc gtctctccat caccgaccgg tgcaacctgc gctgcacgta ctgcatgccg
     5701 ccggagggga tcaaggcgcg cggccacggc gagatcctga gctacgagga gctcgccgtc
     5761 ttcgcgcgcg tcgccgcgga cgtcggcatc accaaggtgc gcatcaccgg cggcgagccg
     5821 ctcgtccgca ccggctgcgc cgacctcgtc ggcatgctca gccgcaccag cggcatcgac
     5881 gacatctcgc tgaccaccaa cggcctgctg ctgccccgtt tcgcggacga gctcgccgcg
     5941 gcggggctca ggcgcgtcaa cgtgagtctg gacagcctcg acccggggcg cttcacgcgc
     6001 atcacgcgtg gcggccgcct cgaggacacg ctggccggcc tcgacgcggc gttcgcggcc
     6061 ggcttctcgc ccatcaagat caacacgctc ctcctgcccg gcgtggagga ggagctcgcc
     6121 gcgttcgtcg acctcacgcg cgagtacgac gtgcacgtgc gcttcatcga gttcatgccg
     6181 ctggacaggc gggtcgccgg cgaagacctc gggggggagg ggcggctgct gccggccggc
     6241 gacgtgctgc gccggctcat gcagcagtac gtgctgatcg gccacgacgg cccctacggc
     6301 cacggcccgg cgcagtactg gaaggtcgcc ggcgcgcgcg gcaccatcgg cttcatcgcc
     6361 ggcgtctcgg agcacttctg cgagacgtgc aaccgcctgc gtctcaccgc cgacggccgc
     6421 ctgcgcacct gcctgttctc ggggcgcgag accgacatcc gtccgttgct gggcgatccc
     6481 gccgtcctgc gcgcggcgat cgagaccgcc gtcgcgggca agacctacga ccgttgccgc
     6541 gaggcgctgg ccaacgagcg gtcgatgtcc cagatcgggg ggtgacgtgg agctcagcca
     6601 ccttgacgag cgcggtcgcg ccgccatggt cgacgtcggc ggcaaggacg acaccgagcg
     6661 cgtggctcgc gccgaagcgg ccgtggtcat ggcgccggag acgctcgcca tcatcatgga
     6721 cgaggccgtg cccaagggcg acgtcatcgc cgtcgcgcgg ctggcgggca tcatggccgc
     6781 gaagcgcacg cacgagctca tcccgctctg ccaccagctg aacctgacca gggtcgcggt
     6841 ggagatcgaa gccgacgagg cgctgcccgg cctgcacatc gtcaccgagg cgcggctgcg
     6901 cggccgcacc ggcgtggaga tggaggcgct cgtggccgcg tcg
//
LOCUS       DASUMX010000253        53334 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_667343, whole genome shotgun sequence.
ACCESSION   DASUMX010000253 DASUMX010000000
VERSION     DASUMX010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 53334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53334
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_667343"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VFZ86_11885"
     CDS             <1..183
                     /locus_tag="VFZ86_11885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643017.1"
                     /translation="LEGDGISVETTTTTFGDFAFEKLPKGKAFTVTVALDGYARQVFE
                     VKTHTDVDLGEVVLAR"
     gene            286..2886
                     /locus_tag="VFZ86_11890"
     CDS             286..2886
                     /locus_tag="VFZ86_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013976523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX5643018.1"
                     /translation="MDATQQGVRRLTNLTTGGPVHVDVEDGRIRRIVPLQLDESDAPS
                     WTIAARGRSFTPPRKTTLSPWTVAHRSTIYAPNRILTPLKRVDFDPQGARNIDKRGES
                     AYEPIGWDEALDMVAAEMIRLKREYGPAAMLSTAGSHHLWGNVGYRFSAYNRFMNLAG
                     FTYAEHNPDSWEGWQWGGAHMWGFAHRLGIPEQYDLLGDALEHTELMVFWSADPETTG
                     GVYGSFESTSRRFWLKELGVKMIFIDPYDSPTAQLFGDKWLAPRPGTDVALGLAVAHT
                     WLDEGTYDKEYIADRTHGFGEWRDYVLGRSDNVPKTPEWAEAECGVPARQIRALAREW
                     GRKRTMLAAGGMGGWGGACRASSGNEWSRMMIALAAMQGMGKPGSNIWSTTSGVPFDP
                     AFFFPGYAEGGISGDTAGTAAGKKLANRMWPDGGTFSNPQHSPEGQTVPRLRIPEAMN
                     HERLEWHGKGFCGSSIESQFREYHYPAAGYPHVQMYYRYGGSFIGTMTETNRYVRAYR
                     EGKIPFVVNQSIWMEGEARFADIVLPACTNFERWDIGDWAHASGYGMHKFEQVNHRLI
                     VLEQRCIEPLGESKSDYEIFAALAGKLGVGDIYTEGGLTELDWVKRIFHASDLPGRVS
                     WDEFAEKGYYLVPVGDDHRATPALRWFAEDRKRDTPDWGPPPWDTVGGKGLATQSGKI
                     EFVSSSLKRFYASGDVVDEERPVLGPQYIESWEGHHTEELTARYPLQLISPHPRFSFH
                     TMGDGKDAWAADVEDHRELKDDGWYYWLIRLNPDDAAARGIADGDLVRAFNDRGSVIL
                     CARVTGKIRPGTTHSAESCSDYAPIGAHGHSPDRAGCVNILTSRRFITPTSPGQACNT
                     CLIEVERWSG"
     gene            complement(2932..3648)
                     /locus_tag="VFZ86_11895"
     CDS             complement(2932..3648)
                     /locus_tag="VFZ86_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020279799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5643019.1"
                     /translation="MLAINDIHTWYGDSYILQGVDMEVRDATVVALLGRNGMGKTTTI
                     RSIIGLTPPRKGSITFNGTELVGLPPNQIARTGIGLVPQGRMIFASLSVTENLTMAAR
                     TGGKDDPWTLEKIYEIFPRLKERARNKGNLLSGGEQQMLTIARALMTNPDLIMLDEPS
                     EGLAPIVVQEVYRVIEKLKAAGQSILLVEQDFGMAMSVADYAYIMSKGSIVYGSAPAD
                     LIDNETVKAQYLGVGTDDEV"
     gene            complement(3654..4418)
                     /locus_tag="VFZ86_11900"
     CDS             complement(3654..4418)
                     /locus_tag="VFZ86_11900"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5643020.1"
                     /translation="MAALLEVEGLSVHFGGLAAVKNVTFTIGEGERLGMIGPNGAGKT
                     TLFNLLTGQLKPSGGKVVFKGQDITHKPVYARTQLGIARSFQITSLFPNESVLTNALI
                     ALQGTRAGRYRVWGVFKRDKALYAAAQGLLESIDLWEKRQEMVSSLAYGEQRKLEMGL
                     SLASGPELLMLDEPSCGLTPTESKDITERIRALGKKITVLMIAHDMDLVFGVAERIIL
                     LHFGEISCEGTCDEIRANQTVKDIYMGSHKASTAAG"
     gene            complement(4402..5475)
                     /locus_tag="VFZ86_11905"
     CDS             complement(4402..5475)
                     /locus_tag="VFZ86_11905"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX5643021.1"
                     /translation="MATRPADLLLGRLMRRAVAAPPVPVEGRSAAALLRTWAPAIAII
                     LLMLVLPSFVPSYIQSLVTKMMIFALFGVSMNILWGYGGIPTFGHAAFFGFGGYACGI
                     LIVHGGISNFFVNLVLVVVLTALLGAGLGIPAFRVYGVGASDNPIYFLLATLAFGELL
                     VRVAISARTLTGGSTGMSGIPWPDLGIPGITVNSSYTYYFLVLAVVVVCLILVYRLVN
                     SHYGYALRGIHDNERRMQALGYNTWLYKYTSYIIAGAFGGIAGALFAYFGGVMVPGNM
                     GMAMTDIVFLIVILGSASIYFGPVIGAVAVVGVEYLASVYLPERWPLIMAALIVVVIM
                     VIPQGIGVMLLDLWRKVFRGRAA"
     gene            complement(5475..6335)
                     /locus_tag="VFZ86_11910"
     CDS             complement(5475..6335)
                     /locus_tag="VFZ86_11910"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX5643022.1"
                     /translation="MSTFIITTLNGVSYAFILFLLAAGFSLTFGVMGVLNLAHGALFV
                     LGAYFGLAIISQLDQFWLGVVVAAAGTGLVGLGIYYFFLRHLYGKVPEQAVLTIGLGY
                     AIANIVLWVWGPFAKMGLTPETFTGVVRVGDLAFPIYRLVICGIGLAVLVFLWWMQDK
                     TRVGAIIRAGMDNKEMTRSLGINYGLVSTLVFLFGAALAGFAGIIGTPVLGAYPSMGD
                     ALLLLTLMVVVVGGTGYIQGTMLGALIIGLLDTFGKAYVPSVALFTSYVIFIVVLLVR
                     PSGLIGRKVF"
     gene            complement(6681..7919)
                     /locus_tag="VFZ86_11915"
     CDS             complement(6681..7919)
                     /locus_tag="VFZ86_11915"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5643023.1"
                     /translation="MQRKVVFAVLALVLVLGAFGALVLAACGSETTDSRSTESAEPAA
                     AATGEPIKIGFDEGFTGFMAVDATMTDKGIKLAIDQLGGQIAGRPVEYIKADNGSDPV
                     QAVDKARQLVENDGIHVMIGPIFSPANAAVTDYLAKSGGGIPSISIYGQPIDNMKTAN
                     GLSFVPSGLFSFAGYQTGKYAAEEMGWKTANCINYEDTAGRQLQEGFNHGFEEAGGKI
                     LSTQFVPIDAVDFSSYLTTLKDADCTYWWIFGNGAAPFVKQYNDYGLEAPLITAMASN
                     LTEPQLRDLGEVGVGLIGGEFYTPEIDNPVNKEFVEAYKAQEDGEYPTMQSFGAWLAV
                     NLFAGAVEANGGDTSPDKLKEAMSSVTIDTPAGPYKMSAYQDMFVGTGNYYMMETKQV
                     GDRIAWVPVFTYEGLLHDQK"
     gene            complement(8243..9064)
                     /locus_tag="VFZ86_11920"
     CDS             complement(8243..9064)
                     /locus_tag="VFZ86_11920"
                     /inference="COORDINATES: protein motif:HMM:NF036639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaF C-terminal domain-containing protein"
                     /protein_id="HEX5643024.1"
                     /translation="MADEALRFIQVGELVKDISEGTLPADDALAGRSLSLHAEDATVT
                     ALSFARGGRLSWEVTAGPGAGETGEETCHITRPREEVAVVDYVASTRRATAVTIVLDL
                     ARSAATTVVGTLPTADQAARGAFALATGGTELTLVAAGIVPAVIDGPFVPGAHPHVPT
                     DDLVGKRVQYVYSQTEAYEHIYLSRDLYTWQCLRGAERGLADTDRCHYRKVGEELYLF
                     VWREKVIPTLGVVLVDWRAKRSNGRLFGYEGSDFGALATTPISARATLLNVTTHE"
     gene            complement(9111..10496)
                     /locus_tag="VFZ86_11925"
     CDS             complement(9111..10496)
                     /locus_tag="VFZ86_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX5643025.1"
                     /translation="MNAEALEAHLMELSWPDAPSMVTEAPGPKVLALMEESARYESFT
                     RGGGGFPVILEQGKGVTVKDADGNMYIDMAAGVAVNAVGRNHPKVLAAIRRQCDVIMH
                     TTDITNPKRIELARKVSGIMPDGLAGECHTAVYQAGSDAVETAIKFARAYTGRSQLIA
                     FHGAYHGVWCGTAAMTTSYNYRHGWGPLIAGVQHLPYAYCYRCPFGLEYPGCDVQCGK
                     YVDDILNGPYTGADDVAAVVIESQQGEGGYIAPPPEFLEMIKAACEKNGCLYIADEVQ
                     SGAGRTGRMWAVQHSKVVPDMLTWGKGMGGDMPMAGVTYRSEMEGALREGSQPSTFAG
                     NAMGCEVAMTNIDILTDPATDLIGRAAVLGEEIKGMFVDAMPNVPVIGDVRGNGLMVG
                     IEVVEDRDTKEPLAGEKVAEIAFKLLNSGILMTPCGRHANVFRFMPPLTVPRDYAVRA
                     TEIMIDILKGY"
     gene            complement(10714..11610)
                     /gene="sucD"
                     /locus_tag="VFZ86_11930"
     CDS             complement(10714..11610)
                     /gene="sucD"
                     /locus_tag="VFZ86_11930"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014703068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEX5643026.1"
                     /translation="MSILVDKSSRVLVQGMTGREGTFHTERMLAAGTSVVAGVTPGKG
                     GQSACGLPVFDTVGEAVQATGADVSVIFVPARFAKAAVVQAAEGGVRVAVLITEGIPV
                     RDMSEAVAELKLDDGRVAERPFVLIGPNCPGIVTPDGCNAGIMAASLFRPGRVGIVSR
                     SGTLTYEIAMGLTRAGLGQSTCVGMGGDPIHGLGFRECLELFEADTQTEAVVLVGEIG
                     GDDEERAAGFAAEHLTKPLVAYIAGFTAPPGKRMGHAGAIISGSSGTAAAKAAALEAA
                     GIPVARRPDETPVLVAEALAAI"
     gene            complement(11607..12761)
                     /gene="sucC"
                     /locus_tag="VFZ86_11935"
     CDS             complement(11607..12761)
                     /gene="sucC"
                     /locus_tag="VFZ86_11935"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF001913.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEX5643027.1"
                     /translation="MDLFEHQGKELFAAAGIPVLPSRVVFTEEEAERAAAELGYPLAV
                     KAQVLTGGRGKAGGVRVVTRPQELGEAVSAILRLIIKDKPVHSVLLEQGASVMRELYL
                     AVTLDRHAKAPLLIFSARGGVDIELVAKQDPAALLRLPLDAVSPDCGGPRDAVVAAAA
                     LGDTQLDEQLGDLVDRLWTLYRERDASLVEVNPLAVVRDPARLVALDAKVTIDDNALY
                     RQPDLAAQRPDEDARERAAREAGVTYLTLDGDVGVLGNGAGLVMSTLDMIGAAGGAAA
                     DFCDVGGGARAERIAAALDIITSDDRVRALLVNIFGGITRGDEVARGVLAWLETTGVD
                     LPLVVRLDGNNAAEGLQLIAAAGIDGLSVTLSAQEAVESVVAAAKTGSRA"
     gene            complement(12794..13576)
                     /locus_tag="VFZ86_11940"
     CDS             complement(12794..13576)
                     /locus_tag="VFZ86_11940"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009261099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX5643028.1"
                     /translation="MSLKVYNLSHTFTQFIPEWPSTPSVNVKVSKFHAKDGVYEVEWE
                     GIMHRCTHMDAPLHVTENTPSIADYPLWRLVGTGVCVDIPKGKWGVITPEDLENATPK
                     IEEGDIVMINTGFHRLWGDTDEYFAYGCGMGAEGARWLVDRKVKLVGYGHQANDHPIA
                     TKLVDHGLGPTQPHLIDEYKEFTGRDAKADFPSWEPAHKILMCEGGIPGIENVGGDLD
                     EVTGKRCTFLALPWRWPGGDGCMVRVLAVIDPDQTFRFGTGQ"
     gene            complement(13605..14411)
                     /locus_tag="VFZ86_11945"
     CDS             complement(13605..14411)
                     /locus_tag="VFZ86_11945"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007989199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX5643029.1"
                     /translation="MTDVVTDPYSGLQLIELSHVWGHGVPSEPGQLDVRMVRAVKHAQ
                     HGVLAWRVTTSLHTGTHMNAPIHMVQRGDDLAAVSPDRLFGNAAVLDIPRKSWEAVTA
                     ADLEAATPAVQDGDIVVIVTGWHHNYSDGLEYFGEAPGLTRDAAEWLIKKDPKLVAVD
                     TPFIDMPLATSMGPHRGGPQMKRLAVEYSAATGRDAKEDFPDWFPAHKTLAAAGIPTV
                     EQVGGDVDLVKGKRATVAATPWKFEHGDACPVRMIAMLDPSGDLRIDAGD"
     gene            complement(14453..15646)
                     /locus_tag="VFZ86_11950"
     CDS             complement(14453..15646)
                     /locus_tag="VFZ86_11950"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014183696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEX5643030.1"
                     /translation="MRDVVIVSAARTALGRVGGAIKSVQPEELAKIVILAAVERAGVE
                     PPSVDEVIIGQTKQSADAANLARVAALKAGFPVEVPAYTVMRQCGSGLQAIHNAVQVI
                     RAEEADIVVAGGVESMSLAPYYLRGARFGFTSGNTVVVDSNTESQPRSQPYEVYGDLT
                     MGLTAENLAEKYDISREEQDAFALQSQERAADAIASGRFKDEIVPVVVPQRKGESVVF
                     DTDEHPRKTSLEQLAKLKPVFKQGGSVTAGNSSGRNDGGACLVLMDAETAKARRIRPM
                     ATIRAQATAAVDPRIMGIGPAPAARKALQRAGIALSDVGLIEVNEAFAAQSLAVVKEL
                     ELGQEIVNVNGGAIALGHPLGCSGARILTTLLYEMRRRGTRYGLAGICIAGGQGMATI
                     VEAVG"
     gene            complement(15662..16438)
                     /locus_tag="VFZ86_11955"
     CDS             complement(15662..16438)
                     /locus_tag="VFZ86_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009261122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5643031.1"
                     /translation="MEDLFSLEGKSAVVVGGAGGMGRAIAAGLIRYGADVALADVNPE
                     ALEQVAAALVEATGKPVRSYVIDCTDEASVKGVVDSTVADMGGRVDVLVNALGYNVKG
                     AATEFPMDEWDKLFSVNVKGVMMSCKHFGAQMVKQGGGKIINLSSVRGQRGALGGNSA
                     YCASKGAVDMITKQLACELAQHKVFVNALGPIITVTPMTAERISNEAARYERMLLNVP
                     MGRMAHVDDLVGPSVFLASAASDFITGTVLYVDGGTMSFV"
     gene            complement(16456..17313)
                     /locus_tag="VFZ86_11960"
     CDS             complement(16456..17313)
                     /locus_tag="VFZ86_11960"
                     /inference="COORDINATES: protein motif:HMM:NF017652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HEX5643032.1"
                     /translation="MKLPRGKVIITASLNGAFVNKAMNPNVPEQPDEIARASRECYDA
                     GAAIVHIHARDEQGKPAGTKEKFAEIQAAVKAECREVIVQFSTGGGTNLSIEERVSCL
                     DADPEMASLNMGTLMRQTGPNAGEPFSNMTKDIEAWAAKMKEMHIKPEMECYSQSMYR
                     DVHNLIKKDLIEPPYYINFVLGMMHQGAIEANPDTLYSMYQFMPQDAYFNTTATGAAE
                     LPITTMGMIMGGCARVGLEDNIYYKKGVLAEGNAQLVERTVRIARELNLEPATPDEAR
                     EILGVVKKY"
     gene            complement(17385..18335)
                     /locus_tag="VFZ86_11965"
     CDS             complement(17385..18335)
                     /locus_tag="VFZ86_11965"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5643033.1"
                     /translation="MDVKKVAVVGAGTMGPGLAQVFACAGHPVSLYSRSAATLEKAMS
                     VTAANMQTFAGHGLLSRDDVSAALARITTTQSVEEAGAGADLVIESIAENLEAKRAVF
                     ADLDRACPDGAIFTSNTSYLDIYQVVPERRLPDTVIAHWFAPPQIIPLVEVVKGESTS
                     DETVELVVALLKKVDRVPVVMEKFVPGFCINRFLRIIGREVFFLLDNGYMTAEQLDLA
                     VKASIIPRAMVLGFVQRYDFTGLDLSLNNLQNSDFLEPPIDNAPSSLVGHVEKGELGV
                     KSGKGFFDYSDRDLREVLRTRDDALIEVFNQSKDLIYKRV"
     gene            complement(18587..19435)
                     /locus_tag="VFZ86_11970"
     CDS             complement(18587..19435)
                     /locus_tag="VFZ86_11970"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX5643034.1"
                     /translation="MAKETRKAGKKKRQPAANGPLEAGAEAFVVRALAKGLAMLGLFD
                     AEHREWTLDEMVSELQLPRMTGYRMARTLQSAGYLVTDTQSGRYRLGPALLAATYLSE
                     GYAELVAIARPYLQALVEETGESATLAVDVDGVAVCVDMLDSPRPHKREVAVGRVIGD
                     TANAHGKMYAASMPDGERERLLSLPHERLTPRTITDPEELAAELRRVREEGVAFDIEE
                     RNLGTCAVAAPVRDQMGHVIGSIGVIVPTGRFGTEQRETCVSAVKASAAALSGFLGYA
                     GADARV"
     gene            complement(19462..21174)
                     /locus_tag="VFZ86_11975"
     CDS             complement(19462..21174)
                     /locus_tag="VFZ86_11975"
                     /inference="COORDINATES: protein motif:HMM:NF013481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HEX5643035.1"
                     /translation="MTDLLNVDLTSGAVAREPLGADLEPLGGHGLTSAIVCGEVHPEA
                     DPLGPGNVLVYAAGLFAGTVVPNGGRLSVGGKSPLTGGIKEANSGGSAARALAELGLR
                     ALKVTGQADETSVLVVTAGGGTLVPAPELAGLGSFATAERLWEQYGDKVSLICTGPAG
                     EMGAKAAAVLVTTPDHHMRAAARGGLGAVMGSKNLKAIVVDAAGGPGVVVADKDALKE
                     ASKALATGIHSHPAMAALEALGSAFLVTVTQSMGCLATRNFSAGTWENAPDISGEHMV
                     EIISARPNGATKHSCMKGCIINCSQVYTDDAGEVITSGVEFETLGLMGSNCHIKDLDQ
                     IARLDRLCDDLGLDTMEVGAAMGVAMEGGLLDWGDGEGAFALLSKLRDGDADARMLAD
                     GCVTTGQRLGVARVPAVKGQSFAAYDPRVLKGTGTTYITSPQGADHTAGNALPSPANP
                     TYDPASAEGQAQISEFLQCYFAAIDSLGMCLFATLPALDMPELQGEFVKAAAAITGEA
                     LPEDYLIRMGDQIVRGERDFNRRAGFTAKDDRPPAFITEEAVLPSGTRYDVAEADLDA
                     MFTS"
     gene            complement(21333..22721)
                     /locus_tag="VFZ86_11980"
     CDS             complement(21333..22721)
                     /locus_tag="VFZ86_11980"
                     /EC_number="4.1.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050371 - tyrosine phenol-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine phenol-lyase"
                     /protein_id="HEX5643036.1"
                     /translation="MTEPRRRSWAEPFKIKMVEPLKMTTREEREAAIREAGYNTFLLR
                     SEDVYIDLLTDSGTSAMSDRQWAGMMLGDEAYAGSRNFYHLESSVQDVYGYKYLVPTH
                     QGRGAEHLLSQTLIKQGDVIPGNMYFTTTRLHQELAGGRFVDVIIDEAHDPTSEHPFK
                     GDVDLAKLQRVIDEVGAERIPYVCIATAVNMAGGQPISLANLRQVRALCSDHGIRIIH
                     DATRVAENAYFIKEREPGYADRTVAEIVREICGLTDGCTMSAKKDALVNIGGFLALNE
                     WEVFEACRNLVVVYEGLHTYGGMAGRDMEAVAIGLRESVDFDHIKARVGQVKYLGEKL
                     QDYGVPIVVPIGSHGVFLDARAILPHLDQDLYPAQALAAELYLDSGVRAMERGNVSAG
                     RDPQTGQNRRPALELVRLTVPRRVYTQAHMDVVAESVAEVWDQRGAVKGLTMVYEPSD
                     LRFFQARFEPMG"
     gene            complement(22771..24165)
                     /locus_tag="VFZ86_11985"
     CDS             complement(22771..24165)
                     /locus_tag="VFZ86_11985"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HEX5643037.1"
                     /translation="MPQFRLHTKALDAEGCHAVHGATLEVLEKTGVEVQHEDALSLLK
                     AAGANVEGSRVCMPAGLVDDALAAAPRSITLASRGVTSGLDLTSGPVYYGTGSDCIFV
                     LGPGPRDRRDGTLADVEEMAALQEKLPNIDFVLSMVHPHELPASVAPVAQFAAMLRGT
                     SKPLIMVPEDARHLALFNEMAATCGGAGSWGIYAMPTPPLTHGRESVDRLAGCARLGI
                     PMVYAGAYLPGATAPASAAACVLLANAEVLSGLVISELVSPGAPFVYGTSQGWMEPRT
                     GHIVYCGAEEMAVQQASADLAAHYGLPSFGTGGCSDSLLLDEQWALEAGMTLLTAASS
                     GVTLLHDLGYVASGTASSYESIVVADELAGWVKAYVAGVTIDEESLAAAEIAAAGPGG
                     THLGRKYSRHVRDFFRPRLISQDSLESWRAAGAASFLERAAERTRELRDAERAYTPGD
                     DALRELDRLVGQAT"
     gene            24382..24927
                     /locus_tag="VFZ86_11990"
     CDS             24382..24927
                     /locus_tag="VFZ86_11990"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5643038.1"
                     /translation="MVGGRLREARRSRGMTLDDVAREAGLTKSFVSAVERGDTSPSIG
                     SLYRICEVLGIPVASLFETAPAPESNVVRHTDVRGVSFGGEGVENYVLSPRAERRAQV
                     IETHVAPGGSPGPEPWSHSGDLIIATVLEGTLEFRFEDHTTALHAGDTISYSPAEPHS
                     WGNPSDDAPAVALFFGMPAEY"
     gene            25004..26884
                     /locus_tag="VFZ86_11995"
     CDS             25004..26884
                     /locus_tag="VFZ86_11995"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5643039.1"
                     /translation="MKTFWRILKLLGDYKWLTILGFALAFTQMGLGLVVPRIIQLTIN
                     NALIGGETGLLVTYGLALFGIAVVRLFVGVGRRLASGKASLGIEYDLRNRMWTHILRQ
                     SFSYFDRWPTGQLMSRAMSDVQNVRMFLGYGLIFFVTNIVMMVAIAAILLVMEWKLAL
                     MSLAFLPLLLLVTVRFSLRLKPILVDVQQKIADVTAAAEENVVGSRIVRIFAREDDEL
                     VKFSDRSYKVFEASVAAARTRAVYIPLIAFIPNLAVAFLLYYGGRQVIEGDLSLGSLV
                     AFYSYLMMLVYPAQVIGWLTGLAQRAIASGERVYEVLDSPLEMTEKPDAQPLRDAFGL
                     TAAQATGDGAAGGDGTALAGAAAPVGGAGPSGAVTFDDVWFSYVDRPVLRGVSLEVPA
                     GHTIALVGHTGCGKTTLTNLVPRFYDTGGGRVLIDGQDVRDLQLADLRRHIGIVNQDP
                     FLFSASIAENIRFGRPEATDDEVRAAARTAQTDDFIEDLPESYETVIGERGFTLSGGQ
                     RQRIAIARALVMDPRILILDDATSSVDVETEFRIRAALQEVMRGRTTFIIAHRPSTIS
                     LAEEVVVLDQGQIVERGRHVELMAAGGVYAHMFGDAEREGCCLDDAGEEKTGPAASGG
                     AA"
     gene            26884..28710
                     /locus_tag="VFZ86_12000"
     CDS             26884..28710
                     /locus_tag="VFZ86_12000"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5643040.1"
                     /translation="MATTSRHSAQHGTGASLEELKGWRAFLHLASELKLSRLARLASP
                     YRRRAALSIVAMVIVTLSSLAVPYLLKVAIDGGITAGNVTVLTWVIVAFVAVSLVNLG
                     ASYLQTYLTSWVGSHIIYDLRRQLFAHLQKLSLDFFSRQKTGWIVSRLTNDIDALDQL
                     VTEGVTSLVTNSLTFIGSIVFLFILDWRLALATLTVMPFLIVATLIFRARSARAYAQV
                     RNKIGDVSAHLQESISGVRVLKAFRREESDYERLKVANAAYREVNMRTVVQSGVYFPF
                     VEFVSTSAVVIVLWYGGSLIGGGSLEIGVLVAFIGYLNSFFDPLQQLSQLYNTFQASM
                     AAVQKIYKVLDTDPDMLDAPGAVDLPDAHGAVELRDVTFGYDPAKPVLHEVDLRIPAG
                     KTVALVGATGAGKSTVIKLLARFYDPQQGEVLIDGHDLREVTQRSLREQLAVVPQEAF
                     LFSGSVLDNIRFARPRASDDEVKRVARIVGVHDFVEGLPDGYETEVQEGGSALSTGQR
                     QLISFARALLADPRILILDEATSSVDAESERRIERAMEVLFSGRTSIIVAHRLSTVRY
                     ADEIVVVDGGRILERGTHDSLVASGGRYAGLYRDWEATGLAL"
     gene            complement(28948..30300)
                     /locus_tag="VFZ86_12005"
     CDS             complement(28948..30300)
                     /locus_tag="VFZ86_12005"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5643041.1"
                     /translation="MTRLADNAAALREPEFRKLFLGQATSVVGVMFTIVALPFAVLEI
                     GGSATDIGIVEAANLVPLAVFLLIGGVWADRLPRQKVMLVADFLRFGLQCTTAALLVA
                     GVAEVWHLALLQVGMGISEAFFRPAAVGLVPQVVSAARLQQANALSGLVMSMSITLGA
                     VFAGLLVAAVGPGWGIGIDGLTYLASAWFLLRLRPAAFRGRREAAAPAAAPGRALSGE
                     EGATGGEIAAAGPGSRGFLRDLTAGWREFTSHTWLWVMVAGVSLFLFAIEGPIQVLGP
                     IVARDVYDGARTWGFTSAAMGVGQIAGGLLSLRWRPRRPMLVIAAGMSLAAVPVALLA
                     MEAPVWTLYVSLAVMGVEWGLYDPFWTTCMQRVVAPGMISRVSSYDYFGSLAFYPAGL
                     ALAGPLADAFGIDTVLWGSATVALLVSAFQVSWRDVRAPKDMGPLEPAGAHAAKSLDG
                     "
     gene            complement(30377..32626)
                     /locus_tag="VFZ86_12010"
     CDS             complement(30377..32626)
                     /locus_tag="VFZ86_12010"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX5643042.1"
                     /translation="MLWSLAFAAGLTASVSVASQLKGGGFTDPSSPSQQGQRQMQERL
                     GFGPASLTIVFASDTLSARGPAFRAQVEESLSGVEEAGLDGLTGIRTAWSSGDASFFS
                     GDGTATFAVLEFDGTSEEVQAQIPALRLALEPTELTTLLTGDPAVYAELEQRSADDLR
                     KAETYTIPVAVLVLVLIFGTLVAAALPVIGGGMAVTVTLGLFWLIAQVLDISVFALNV
                     ATLLGLAVGIDYALFMVGRFREELAAGVAVAEAVETTVATAGRSIFFSGLTVVVGLLG
                     LMIIPYMSLRSMGLGGALVVLVSVLAALTLLPALLGLLGPRVDALRVIRRKNGGGVFW
                     TRWSDWVMRHPVPVLIGTIALVVVFAWPVLRIESDIPGATALPRDSEARKGYDLLLRR
                     FDAAALSPIEVLVTWEGEDDPFAPANLARLHAFGRELEALPGVDRVTSIVTLPGMESA
                     AGAAAFWQGVEAAAAASPREDGALQTEASGLPGLVQGMLGAEQVKGARKLAAATTAPG
                     TALLRVVPEAPPTSEEAQALAVDISDAGGPPGTTTYVAGASLTVHDFVDAIYSRFPWI
                     IAFVVGVTGLVLFLMLRSVVLPVKAVVMNVCSLLAGYGAMVWIFQEGNLEGLFQFTAT
                     GAIDAELPVVLFCTVFGVSMDYEVFLLSRMREEWDATHDNVRAVSFGLARTGRIVTSA
                     ALIIVVVGLSFAFTSIVVTKAIGVGLAVAVALDATVIRVLMVPAAMRLLGRWNWWLPS
                     WLAWLPRIE"
     gene            32761..33198
                     /locus_tag="VFZ86_12015"
     CDS             32761..33198
                     /locus_tag="VFZ86_12015"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX5643043.1"
                     /translation="MPGPVERTSAGGVVVRTDDGVEVCLIRPVGRSVWGLPKGGVEEG
                     ETLAETALREVAEETGLAGVVEQELGSIDYSFYSREQGGRIHKTVHYFLVRATGGDTS
                     QHDHEVSDAKWVRLRQALRLMTYPNEREMVRRAAEALGRPVTD"
     gene            complement(33208..34056)
                     /locus_tag="VFZ86_12020"
     CDS             complement(33208..34056)
                     /locus_tag="VFZ86_12020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643044.1"
                     /translation="MDLTRDISAADLPFDEEAADLWEPAFSHLAEAIAGSESAFLDAL
                     ESAGRGAGITQKVPMAELLDAYSRGGEVVRAGLLRRGGDGAEAAVRRLLALEHAALTR
                     VAAGYAAGLTDTIDRLRRDAVEASPLDADSGAMKPAEFGERLSLEVERCQRMDLPLGL
                     VELAVEGDDGEWRATGSSSRAEVGRLGACVRENLRGYDSVGLTDGGDLVLVLPDISRR
                     GLAGAAERLRRELDACGGRHGATPELTFALAHYDFVDVNAQEMLTVLGRSVEQAREAR
                     QPIVWA"
     gene            34176..34982
                     /locus_tag="VFZ86_12025"
     CDS             34176..34982
                     /locus_tag="VFZ86_12025"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5643045.1"
                     /translation="MDVTLLGTLGWMPRGARETTCFAVREGPALLVFDAGTGLRRLLD
                     PEHAGLLEDAREVHLFLSHYHLDHVCGLAYLSGVFAGRDVVLHPPGRELTGVDPLAAV
                     AELVRRPYNPVDLADMKRVRVEPTAPGENDVADHVVSVRAQQHSDTSVSFRLDDALVI
                     ATDTQVDPAAVPFAEGAGLWLHEAWYWSGDPGLAGVPGELRPGYSAHSEATAVAGLAA
                     QAAVARLILVHLNPLAGEASYLPMRDAARAVFPRTDVVADGTVATTAASV"
     gene            35026..35556
                     /locus_tag="VFZ86_12030"
     CDS             35026..35556
                     /locus_tag="VFZ86_12030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643046.1"
                     /translation="MIRFRDKRFLAVAIVVAALLVFALPAFLAFRYSAPEQRGQFLSR
                     PWRGWSFAYAALAVPGGAELKTSGMALRKADWIYKGTAVDPREVQLLFVSSKRPYTFA
                     HSIDGRTVTSTVVPSYRFIWQVQGTVGTISDKADTVVALLDYDSGRLLYDVRDDLTPD
                     ELAPRPADGASPSPAP"
     gene            35585..36400
                     /locus_tag="VFZ86_12035"
     CDS             35585..36400
                     /locus_tag="VFZ86_12035"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEX5643047.1"
                     /translation="MIIATAFYETNWFKAVVYVVIAIVVARVVDFVLARRDRAMLRLL
                     GKQPDHADRTRYIMIRRLVFIGILFIGVGIALIQIPAVSTLARAMLASAAIIAGVIGI
                     AARAPIANLVSGIMIAFSQPVRLNDYISVDGEFGTVEQISLIYTYIRAGDGRRVVIPN
                     EAFAMKAVHNYSMGSPGGMVHVDLSLPLDADVERIAQAMLEVGESLAPAPVGKQVSVE
                     VADISGGGVTLRLHAWASDPLRRREIASDLRAALLRRLTGDGLLGGEEAGGGD"
     gene            36387..37733
                     /locus_tag="VFZ86_12040"
     CDS             36387..37733
                     /locus_tag="VFZ86_12040"
                     /inference="COORDINATES: protein motif:HMM:NF016104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000009 - alpha-1,6-mannosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0004376 - glycolipid mannosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006506 - GPI anchor biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyltransferase family protein"
                     /protein_id="HEX5643048.1"
                     /translation="MEATDVQGGSEDAAGGGEAAAPTVEAVAADDDGSAAGAEPDASL
                     PRGGRPLTWALATAIGSRVLIFAVAFAAAALIGIGGLPVGWRFPLRAEVFSGWLGSLF
                     NPWAHWDGVWYIKIATSGYAEADGSTAFFPLFPMLLRYVGVLLDGNLLITGIVISLLC
                     YAGCVWLLYRLVRRDFDEKIAARAVIYLAIGPLSFFLQAVYTESLFLLLSLACFVFAR
                     EGRWRLAGVMGLLATLTRSTGVLLLIPMAFYYYKRRGWDFRKTDSHVANLLMVVEGLL
                     VWMTYLALAFGKPLAFSSAQAQWERGLGAPNYTVTRAVVATVWGLRKVVSAEYYRWLW
                     EAPRPGSEYSNLATNVLNLLFLAAAALLLWYGARRLPGAYSWYALAALAYPLFFPSKY
                     VPLMSYPRFTLTVFPLYVALALYTSDRPRAHKVVVSLGVVLLIAFTAKFALFSWVA"
     gene            complement(37750..39399)
                     /locus_tag="VFZ86_12045"
     CDS             complement(37750..39399)
                     /locus_tag="VFZ86_12045"
                     /inference="COORDINATES: protein motif:HMM:NF021379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HEX5643049.1"
                     /translation="MSRRRRTAKAPTRMTGPAGGFFAPAPIEELDDERMKILLRRRAL
                     YVIGGAILFTVVYALLSWLKYRAYMDARYDLGNMVQAVYNTAHGHFLEITTGDLEPRQ
                     MSRLGSHVDPILALFALPWLVWPSPVMLLVGQAAIAATGAWPAFRLGTRVMRDPTAGA
                     FFAGAYLLYPALGFLVLNEFHPVALATPLLLWAFLYMEEDRWVRAAVFLVLAAACKET
                     VPLVIAFMGAYFAVRKRSPWPLVVTLLAVAWFAVAVWVVIPHYNGDQSAFISRYGDYG
                     SGAGEVAKNALLHPGQTAEDVFAESNLRYLLSLLWPLGFTSVLSPLTALIALPEYALN
                     ALSATVFQRRIEFHYTALEIPFLFAAAVLGVSRLWRWLGGGLRRSEELMRGERIQRST
                     LALLVLLCALAGNFVLGPLPFSLPGAAYGGADYARTAHDAVLDEAIALIPEDARVSAN
                     NNAGAQLAARRVAYIFPYFAKADWVIVDQRHPFFYDREDEEMHSLALGRLVLDPNFRS
                     VFAKDGVLVFERIGAGGGSVPGGQTPAATPTPGETASPAAP"
     gene            complement(39396..40391)
                     /locus_tag="VFZ86_12050"
     CDS             complement(39396..40391)
                     /locus_tag="VFZ86_12050"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HEX5643050.1"
                     /translation="MSAGETAGRRPEEAPREPDGAVRRPSSRRRLFVRLAQAAIVAAT
                     VYFLAAYLARSWGSIQEFDWTFDAGWLAASGAAFLVYYFAQAGFWWLLLRGCGAPSPF
                     WPAASVWGKSILARYVPGNVFMFVGRAWMSHSRGLVIERVTAAMVYEQALGVCGALLA
                     VAVLFPFWEYRPGVTALSLIVIPVLVALMHPRVFGPLAGWALRLLHRPPLEVTVGFGS
                     VLVMLILFTLDWLVAGTGAWLLARAVTGLGVDALPLVIVAYALAYVAGMVAFIIPSGI
                     GVREAVLTASLSRELPGGVALTWALLLRLWVTAMELVFVGVVVGVELLVRRRGAA"
     gene            complement(40388..41506)
                     /locus_tag="VFZ86_12055"
     CDS             complement(40388..41506)
                     /locus_tag="VFZ86_12055"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5643051.1"
                     /translation="MTRAVAYFGTYEPDYPRNAVLIAGLREHGVEVHEFHAALPRLSA
                     AEMAGASGAARLAAGVLAAHARLFAQHRVVLEVDAVVVGYPGHFLVPFGRVLAAFRHA
                     RLVFDPLVSLLDTFAGDRALVRADGARAAAIRAVDEVAFRLPDLVLADTWAHAAYYQG
                     LFGLARRRVAVVPVGALPEPRADGAARELGPGEPLTVLQYGKWSPLHGAEVVLGAAEL
                     LRAEPFRFVLIGEGQLSADLRAAIAARSLTNVEWLGQLPAGELRRHTLAADVCLGVFG
                     GSEKAGRVVPNKVFDALACGRPVISADSDGAREWLRDGETALLTPAGDAAALAAALRR
                     LLDERERARLGAAALVLYRRAFTPTAVAGALLAALERA"
     gene            complement(41503..42531)
                     /locus_tag="VFZ86_12060"
     CDS             complement(41503..42531)
                     /locus_tag="VFZ86_12060"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX5643052.1"
                     /translation="MGPLLSLVIPVYNEADSLVPLVAEIDEALAAYGSPYEIVFVDDG
                     SSDGSFEVMQGLAEARDDVRVVKLRRNFGKAAALSHGFAAARGDTIVTMDGDRQDDPA
                     EIPNLTAPLSQGFDLVSGWKQRRQDPLNKTLPSRFFNWTVRRSSGIPLHDFNCGLKAY
                     RREVVDTISVYGELHRYIPVVAAQAGFRVTEAKVSHRRRTAGRSKYGWQRYMRGYLDL
                     LTVLFLGRYQHRPQHLFGGFGTLLILVGVLIEVYLTIDKLVFGNPIGQRPLLLLGALL
                     IIVGVQLLSLGLIGELIANSRARRGPDPAQIAALLGPETSAAAPEPPAEGGGDVASRG
                     AAGAAAQP"
     gene            42608..44425
                     /locus_tag="VFZ86_12065"
     CDS             42608..44425
                     /locus_tag="VFZ86_12065"
                     /inference="COORDINATES: protein motif:HMM:NF012647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HEX5643053.1"
                     /translation="MSRKTWLVLAVAALIAVSFAASGIAAKKSSEDRPRYVTRGLDYL
                     HARQLDNGGFSTAANTAWAVLGAVASGERMGSSAWTKKGKNPFEYLQSVNHEAAATSA
                     TVDNAPVYYARMIMAYVAVDRRDRVFVAGTPHVDLLAKLYTYQDMADGSSTKGSFSPS
                     SSTRNFDAVNTTSWAALSMYNFGLSTEERFLLAETWLAGMQSGSDGGFPAEAGKSSDC
                     RTTALAIQALSVGPGSLSWDAAAARQFLKDNQNADGGFPVSPGGRTDAEATSAAIQAI
                     LALGEHPDDAFWMAGINTPTYALGVLQQTNGAYRLTSRERLRPLAVTSWALTAMRRKS
                     FASFPRNIGVAETAFKFRPVFRTVSPKNGAKFKSHVVLIRATYTDFYPKGTGIRPSAC
                     RLYVNNENKSRPADIEKYGLRLLLKNVPNGAHTYKIELRDQAGNVKVIERRFTVAVPS
                     PSPTYTPAPRPTYNPGPVYPTVLPTSVPTPRPYTTPTPTPTPTPYSTVTPYPYYSPSP
                     AASPVVTGSPLPSPSASASPAGAGDGEEGSAAGFVGGTLLAMLPIGAVISYLLLQRRE
                     ESLAAASQGTVLDGGGSAWDRFKQTLARSKDLTRPSSRE"
     gene            44433..45440
                     /locus_tag="VFZ86_12070"
     CDS             44433..45440
                     /locus_tag="VFZ86_12070"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX5643054.1"
                     /translation="MNDLQQLIYDIARILLWPVLIAAVICLVWVLIELGVLLYELWLR
                     FRYRDLDALEVRALRARKAFRDGKPRTAYRYLQENTYSLVVVRFLYDLIRNYQTERLA
                     AKPLKLLQEYEFYTIKRLERTRILVRIGPMLGLMGTLIPLAPALVGLASGDVQELSDN
                     LVTAFSVTVIGLLIGGLAFLVSIVRDRLYSQDISDMEYLLELLEGSDVRLRSGRRLSK
                     TGVWDTEAPVEYEVADEDAERTAIITLPATPDEPLLPVATTPGRNGGSGQAAAQRSPA
                     VPAGPAPAARPDDGVGLTFDDPSMDWTRDDDPFAGLGLIDPEGTDDPKPRGSGGPLRR
                     R"
     gene            45444..46046
                     /locus_tag="VFZ86_12075"
     CDS             45444..46046
                     /locus_tag="VFZ86_12075"
                     /inference="COORDINATES: protein motif:HMM:NF021440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2149 domain-containing protein"
                     /protein_id="HEX5643055.1"
                     /translation="MPANDDHSRLQNLGTVMGAPESGLRYMRRRRVGTADRNGDPLYG
                     LANLFVVAIALAVGLLVAALAAAGVSGLLTSENMTIVTNPGTPEMQVIVKEGDQITKL
                     DMQSGAEVAGVGTLIGSFYRLADGTVVYVPAGTTAPTGSTPVPGTTPAPVVTPTPGIT
                     PTPGATPTPGATIPPTAPPTPGTTAFPTPTPAVTLTPASL"
     gene            complement(46053..47036)
                     /locus_tag="VFZ86_12080"
     CDS             complement(46053..47036)
                     /locus_tag="VFZ86_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016354084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEX5643056.1"
                     /translation="MSDARDPDTESQSAGRSGGVGKKALGKGLGALIGESRAALGRSG
                     AVPTLGEGRLRELPLADISVNRRQPRRTFADDELRELAASIEALGVVQPVVVRPLHPE
                     GQAAAGVPAPTGIRFELIAGERRLRAAKLAGLDYIPALVRPADEISSLEIALAENVAR
                     EDLNAIEEALAYAALVDEFDLTHERIAQLVGRSRVAVTNLLRLLDLPDEVQAMIERGD
                     LMEGHGRALLALPDHGERRRVARLAVADGLTVRQVEALVRGLGEKGTDMAAPAPRPAA
                     GRDFTELADELYGVFDAPVKIRSGKRGGSIQIGFKDAAELQRLVDLLRSLG"
     gene            complement(47029..47790)
                     /locus_tag="VFZ86_12085"
     CDS             complement(47029..47790)
                     /locus_tag="VFZ86_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEX5643057.1"
                     /translation="MTRVIAVTNQKGGVGKTTTAVNLAACLAEAEFDVLLLDMDPQAN
                     ASSGLGVRPGDPRLTSYGVLGGAAGIAEATVTTVLRHLSIVPACPDLAGAVVELANAD
                     GNEFVLEYALAGHVETYDFVFIDCPPSLGVLTVNSLVAAQEVLVPVQAEYYALEGLAA
                     LLETIGLVQEHLNPGLTILGVLVTMYDGRTRLGREVAGEVREHLGEHVFQTIIPRNVR
                     LSEAPSHGLPISRYDATCAGSDAYFDLAKEVVSRE"
     gene            complement(47907..48644)
                     /locus_tag="VFZ86_12090"
     CDS             complement(47907..48644)
                     /locus_tag="VFZ86_12090"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070043 - rRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEX5643058.1"
                     /translation="MSTDFRTRAALERVAATHSLEVEQTDALSAFVELLLGWRRANVT
                     GLTDPVAVVDTLIGDSLALLSVPQLRQREAGAWLDLGAGAGIPGIPLAVAVPDATVTL
                     LDAVAKKCAFLEAAVTAAGLTGRAHVVCARSEHHASAGAPGRDAYTVVLARAVSELPV
                     LVELAAPLLAEGGVLLASKTRRALEEEGEAAAVAAGRCGLAPEPPVVLSASPLDEAVC
                     VVFRKREPTADWLPRREGVAGKRPLGV"
     gene            complement(48662..49696)
                     /locus_tag="VFZ86_12095"
     CDS             complement(48662..49696)
                     /locus_tag="VFZ86_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019634217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HEX5643059.1"
                     /translation="MSAPLTLFASDNYAGAHPAVLEAVASANEGWAPAYGDDPWTGRL
                     RERLREVLGDVEAFPVFNGTGGNVTVLAALLRPYEAVICPETAHINVDECGAPERIAG
                     AKLVDVPTPDGKLTPELLRPRLVGFGDQHHVQARVVSISQSTELGTVYAPQEIAALAE
                     TAHEAGLLLHMDGARLVNAAQALGLGLRELTTDCGVDVLTLGGTKSGLLGAEAAVFFR
                     PELAAGYLYARKQGTQLASKMRYISAQLLRLFEGDLWRETAGHANAMARRLGDAVATT
                     PGVTLAYPVQANAVFAALPAAVIERLHERYHFYVWDEDAGVVRWMCSWQTSAGDVDAL
                     AGALREAVSG"
     gene            complement(49693..51762)
                     /locus_tag="VFZ86_12100"
     CDS             complement(49693..51762)
                     /locus_tag="VFZ86_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6421 family protein"
                     /protein_id="HEX5643060.1"
                     /translation="MLDALRQRTVAKVLFDEHHGEAWSIRPEAAARMRPSHPAAASYA
                     TAAAELTARDFDAVTTAGRPLDEVALADVDVLVIAHPSDPKWERTVGADSPVFSPAEI
                     AAVQDFVARGGGLVVLGEEEEDKYGGNLNDLLAPFGVRFENTIVFEYDPGDGVPSWVV
                     GESAPKPADPGILHGVGEVGFYRAGALSTDDPGAVVLRTRATADPPGAALAAATQYKE
                     GRVVVVADSDLFGDDYLSRRDNRRLWMNLLYWVSLAAFRADATPIVSEAAQDPAWLHL
                     KEATGALRLLQEPKGEVDLSRHDAGEVRALVVAMAEAIADLAPRFPHQKEYLAQVVAD
                     LDAWVESGCGKPDFKDSLALFRPDLERRDGVEHLVVFPMYTPNGSPDTRFEALITRTP
                     WPEFVSQIERELYDNPKFVPVQLVDNTAGYDSECAVLFPETVSVAERPTNHFGAIFCD
                     RESARYRRTTLWGAEVLDIDLPPDARALASSPDLALETYILWDMIHDRWHSHGDLPFD
                     PFMIRQRLPYWMYSLEELRVDLATYGTADELAASFPFARYVKYAILFDRLLRFPITGN
                     RVRNYDGLGGQLLFGFLHAHGVVRWTDNRLLIDWERIDSAVAELRGQVEQLYRHGIET
                     SKVTYWIAAHDLISPYVTPNVGSQWRKEARVYSDEAEPRAWIDRVLDDEFPLNMFYES
                     LKKKVAT"
     gene            complement(51905..52327)
                     /locus_tag="VFZ86_12105"
     CDS             complement(51905..52327)
                     /locus_tag="VFZ86_12105"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5643061.1"
                     /translation="MEDYVKLDIPAKAEFVSLGRLALSGLLRSRGGYSDDAVADLKLA
                     LTEACSNSVRHAYDHDQGQVHLEFTVHSDCVTVLIKDQGGGFHEDDDDCPECRTLTDQ
                     LAEGGMGISIIRAVVDDFDLRKPDEGGTVLVLTKRRDL"
     gene            complement(52396..>53334)
                     /gene="amrS"
                     /locus_tag="VFZ86_12110"
     CDS             complement(52396..>53334)
                     /gene="amrS"
                     /locus_tag="VFZ86_12110"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HEX5643062.1"
                     /translation="SGVCRARVNRDGVLYAITYAKVCSVAADPIEKKPLYHFFPGTTV
                     LSLGSLGCNFACRHCQNWQIAHADPAEATRDLRDLPIRNLSVLAANNECQGVAWTYNE
                     PTIWLEYILDGAQAAHEHGLYTVMVTNGYITDDALSILGPHIDAYRVDVKGFTDQQYK
                     DLCKIPDLTPVLESAERAKTEHGCHVEIVTNVIPTFNDDAATLRGIAGWIVERLGAET
                     PWHVTRFMPHLEFAHLPPTPIATLEAAVKTGHDAGLEFVYLGNVPGHEGENTVCPNCK
                     RLLVRRTGSAVEDVALRGTFCAMCGQNVNVRTSLKK"
BASE COUNT         8202 a        18621 c        18451 g         8060 t
ORIGIN      
        1 ctcgagggcg acggcatctc cgtggagacg accacgacga cgttcggtga cttcgccttc
       61 gagaagctgc cgaagggcaa ggcgttcacg gtcaccgtgg cactcgacgg gtatgcgcgc
      121 caggtgttcg aggtgaagac ccacaccgac gtggacctcg gcgaggtcgt cctcgcgagg
      181 taggcgcggc aaggtccgtg atatggacgt cctattccgt gcaatggacc gttcggtgcg
      241 ctacgatgcc ggcacgccaa cgaacgcctg ggtggtgacg ggagcatgga cgcgacgcag
      301 cagggagtca ggcgactcac caatctgacc accggcgggc cggtgcacgt ggacgtcgag
      361 gacggccgga tccggcgcat cgtcccgctg cagctggacg agagcgatgc gccgtcctgg
      421 acgatcgccg cgcgcggccg ctcgttcacg ccgccgcgca agacgacgct ctcgccgtgg
      481 accgtcgccc accggtccac catctacgcg cccaaccgca tcctcacgcc gctcaagcgc
      541 gtcgacttcg acccgcaggg cgcgcgcaac atcgacaagc gtggcgagtc ggcctacgag
      601 cccatcgggt gggacgaggc gctcgacatg gtcgccgccg agatgatccg cctcaagcgc
      661 gagtacgggc cggcggccat gctgtccacc gccggctcgc accacctctg gggcaacgtc
      721 ggctaccgct tcagcgccta caaccggttc atgaacctcg cgggcttcac ctacgccgag
      781 cacaaccccg acagctggga ggggtggcag tggggaggcg cgcacatgtg gggcttcgcg
      841 caccgcctcg gcatccctga gcagtacgac ctcctcgggg acgcactcga gcacaccgag
      901 ctgatggtct tctggtccgc cgaccccgag accacgggcg gcgtgtacgg ctctttcgag
      961 agcacctcac gccgcttctg gctcaaggag ctcggcgtga agatgatctt catcgacccg
     1021 tacgacagcc cgacggcgca gctcttcggc gacaagtggc tggcgccgcg gccggggacg
     1081 gacgtggccc tgggcctggc cgtcgcgcac acctggctcg acgagggcac ctacgacaag
     1141 gagtacatcg ccgaccgcac gcacggcttc ggcgagtggc gggactacgt gctcggcagg
     1201 agcgacaacg tccccaagac gccggagtgg gcggaggccg agtgcggcgt cccggcccgg
     1261 cagatccgcg ccctcgcccg cgagtggggc cgcaagagga ccatgctggc ggccggcggc
     1321 atgggcggct ggggcggcgc ctgccgggcc tccagcggca acgaatggtc gcggatgatg
     1381 atcgccctgg ccgccatgca gggcatgggc aagccgggca gcaacatctg gtccaccacg
     1441 agtggcgtgc cgttcgaccc ggcgttcttc ttccccggct acgccgaggg cggcatctcc
     1501 ggcgacaccg ccggcacggc cgccggcaag aagctcgcca accgcatgtg gccggacggg
     1561 ggcacgttca gcaacccaca gcattcgccc gagggacaga cggtcccgcg cctgcgcatc
     1621 ccggaggcga tgaaccacga gcgtctcgag tggcacggca agggattctg cggctcgtcc
     1681 atcgagtcgc agttccgcga gtaccactac cccgcagcgg gctacccgca cgtgcagatg
     1741 tactaccgct acggcggctc gttcatcggg acgatgaccg agaccaaccg ctacgtgcgc
     1801 gcgtaccgcg agggcaagat cccgttcgtg gtcaaccagt cgatctggat ggagggcgag
     1861 gcccggttcg ccgatatcgt cctgccggcg tgcaccaact tcgaacgctg ggacatcggt
     1921 gactgggctc acgcctcagg ctacggcatg cacaagttcg agcaggtcaa tcaccggctc
     1981 atcgtgctcg agcagagatg tatcgagccg ctcggcgaga gcaagtccga ctacgagatc
     2041 ttcgcggcgc tcgcgggcaa gctcggcgtg ggcgacatct acacggaggg cgggctcacc
     2101 gagctcgact gggtcaagcg catcttccac gccagcgacc tgcccgggcg cgtctcgtgg
     2161 gacgagttcg ccgagaaggg ctactacctg gtgccggtcg gagacgacca ccgggccacg
     2221 ccggcgctgc gctggttcgc cgaagaccgc aagcgcgaca ccccggactg gggcccgccg
     2281 ccgtgggaca ctgtgggcgg caagggcctc gcgacgcagt ccggcaagat cgagttcgtc
     2341 agttccagcc tcaagcgctt ctacgcgtcc ggtgacgtgg tcgacgagga gcgcccggtc
     2401 ctgggcccgc agtacatcga gagctgggag ggccaccaca cggaggagct caccgcgcgc
     2461 tacccgctcc agctcatctc gccgcacccg cgcttcagct tccacaccat gggcgacggc
     2521 aaggacgcct gggctgcgga cgtcgaggac caccgcgagc tcaaggatga cggctggtac
     2581 tactggctca tccgcctcaa ccccgacgac gcggcggcgc gcggcatcgc cgacggcgac
     2641 ctggtgcgcg cgttcaacga ccgcggctcc gtcatcctgt gcgcgcgcgt gaccgggaag
     2701 atccggccgg ggacgaccca ctccgcggag tcctgctccg actacgcgcc catcggcgca
     2761 cacgggcatt cgcccgaccg cgccggctgc gtcaacatcc tcacgtccag gcgcttcatc
     2821 acccccacct cgcccgggca ggcgtgcaac acctgcctca tcgaggtgga gcgctggagc
     2881 gggtgagacg gagccgccgt gtgcgccgct tcccgcggcg catctggctg gtcacacctc
     2941 gtcgtcggta ccgactccga ggtactgggc cttcacggtc tcgttgtcga tcaggtccgc
     3001 gggcgcggag ccgtagacga tggagccttt gctcatgatg taggcgtagt cggccacgct
     3061 catggccata ccgaagtcct gctcgacgag gaggatggac tggccggccg ccttgagctt
     3121 ctcgatgacg cggtacacct cctggaccac gatcggcgcc agcccctccg acggttcgtc
     3181 gagcatgatg aggtcggggt tggtcatgag cgcgcgcgcg atggtgagca tctgctgctc
     3241 gccgccgctg aggaggttgc ccttgttcct ggcgcgctct ttcagccgcg ggaagatctc
     3301 gtagatcttc tcgagcgtcc acgggtcgtc cttgccgccg gtgcgagcgg ccatggtgag
     3361 attctcggtg acggacagcg aagcgaagat catccggccc tgcggcacca ggccgatgcc
     3421 cgtgcgcgcg atctggttgg gcggcagccc gacgagctcg gtgccgttga aggtgatgct
     3481 gcccttgcgc ggcggagtga ggccgatgat ggagcggatc gtcgtggtct tccccatgcc
     3541 gttgcggccg aggagcgcta ccaccgtggc gtccctgacc tccatgtcga cgccctgcag
     3601 gatgtagctg tcgccgtacc aggtgtggat atcgttgatc gccagcatcc cgatcacccg
     3661 gccgcggtgc tcgccttgtg cgaacccatg tagatgtcct tgacggtctg gttggcgcgg
     3721 atctcgtcgc aggtgccctc gcacgagatc tctccgaagt gcagaaggat gatgcgctcc
     3781 gcgacaccga agacgaggtc catgtcgtgg gcgatcatga gcacggtgat cttcttgccc
     3841 agtgcgcgga tgcgctcggt gatgtccttg ctctcggtcg gcgtgaggcc gcagctgggc
     3901 tcgtcgagca tgagcagttc cggcccgctg gcaaggctca gtcccatctc cagcttgcgc
     3961 tgctcgccgt aggccagcga gctgaccatc tcctggcgct tctcccacag gtcgatggac
     4021 tcgagcaacc cctgcgccgc cgcgtacagc gccttgtccc gcttgaagac gccccagacg
     4081 cgatagcgtc cggcgcgcgt cccctgcaga gcgatgagcg cgttcgtgag cacggattcg
     4141 ttggggaaga ggctggtgat ctggaacgac cgcgcgatgc cgagctgggt gcgcgcgtac
     4201 accggcttgt gggtgatgtc ctggcccttg aagaccacct tgccgccgga cggcttgagc
     4261 tggccggtga gcaggttgaa gagcgtggtc ttgccggccc cgttggggcc gatcatgccg
     4321 aggcgctcgc cctccccgat cgtgaaggtg acgttcttga cggcggcgag gccgccgaag
     4381 tgcacggaga ggccctcgac ctcaagcagc gcggccacgg aacaccttcc tccacaggtc
     4441 gaggagcatc acgccgatgc cctgcgggat caccatgatg accaccacga tcaaggcggc
     4501 catgatgagc ggccagcgct cgggcaggta cacgctcgcc aggtactcga cgccgacgac
     4561 cgcgacggct ccgatgacgg ggccgaagta gatcgaggcg ctgccgagga tgacgatgag
     4621 gaagacgatg tcggtcatgg ccatgcccat gttgcctggg accatgacgc cgccgaagta
     4681 ggcgaagagc gcgccggcga tgccgccgaa cgcgccggcg atgatgtacg acgtgtactt
     4741 gtagagccag gtgttgtagc cgagcgcctg catgcggcgc tcgttgtcgt ggatgccgcg
     4801 gagcgcgtag ccgtagtgcg agttcaccag cctgtagacc aggatgaggc acacgacgac
     4861 gacggcgagc acgaggaagt agtaggtgta cgacgagttg acggtgatac ccggtatccc
     4921 gaggtccggc cagggaatgc ccgacatgcc ggtggacccg ccggtgaggg tgcgcgccga
     4981 gatcgcgacg cggaccagca gctcgccgaa cgccagcgtc gccaacagga agtagatcgg
     5041 gttgtcggag gcccccacgc cgtagacgcg gaaggcgggg atgccgagtc cggctccgag
     5101 cagcgcggtg agcacgacca cgagcacgag gttcacgaag aagttggaga tgcccccgtg
     5161 gacgatgagg atgccgcagg cgtagcctcc gaagccgaag aaggcggcgt gaccgaacgt
     5221 cgggatgcct ccgtagcccc agaggatgtt catgctcacc ccgaagaggg cgaagatcat
     5281 catcttcgtg acgaggctct ggatgtacga cggcacgaag ctgggcagca cgagcatcag
     5341 gaggatgatc gcgatcgccg gggcccaggt cctgagcagc gcggccgcgc tgcgcccctc
     5401 caccggcacc ggcggggcgg cgaccgccct gcgcatcaga cggccgagca gcaggtcggc
     5461 gggcctcgtc gccatcagaa gacctttctt ccgatgaggc cgctcgggcg gaccagcagc
     5521 acgacgatga agatcacgta cgacgtgaac agcgccaccg acgggacgta cgcctttccg
     5581 aaggtgtcga gcagcccgat gatgagcgcg ccgagcatgg tgccctggat gtagccggtg
     5641 cctccgacca cgacgaccat gagcgtgagc aggagcagcg cgtcacccat ggacgggtag
     5701 gcgccgagga ccggcgtgcc gatgatcccg gcgaagccgg cgagcgcggc gccgaacagg
     5761 aagacgagcg tgctgacgag gccgtagttg atgccgaggc tccgcgtcat ctccttgttg
     5821 tccatgcccg cgcggatgat ggcgcccacg cgggtcttgt cctgcatcca ccagaggaag
     5881 accagcacgg caaggccgat gccgcagatg acgaggcggt agatcgggaa ggcgaggtcg
     5941 cccacgcgca cgacaccggt gaacgtctcg ggtgtgagcc ccatcttggc gaagggaccc
     6001 cagacccaga ggacgatgtt ggcgatggcg tagcctaacc cgatggtgag caccgcctgt
     6061 tccggtacct tgccgtagag atggcgcagg aagaagtagt agatgccgag gccgaccagc
     6121 cccgtgccgg ccgccgccac gacgacgccc agccagaact ggtcgagctg gctgatgatc
     6181 gcgagaccga agtacgcgcc gagcacgaag agggcgccgt gcgccaggtt gaggactccc
     6241 atgaccccga aggtgaggga gaacccggcg gccaacagga acaggatgaa cgcgtacgag
     6301 acgccgttca gcgtcgtgat gatgaatgta ctcacacgcc cccctggcgt cgagacgagt
     6361 actccgtcgg cgatcccggc ttcacaccgg ctggcacggg cacgctctgc ctgacctcaa
     6421 ccctcctgcc gcgttacggg aatgatatac cgatatcagt ctttgacaag cgacctgccg
     6481 ggatggccct cccgcccgcc tcaggctgtg tgctggctgc ccgtgtcagg caccgttcgc
     6541 cgcgccttcg ggccgtcatc tcccctgata tcgggagtac agccacacgg gggcaagggg
     6601 gagggggctc acggtcttgc gccgtgagcc ccctccgtgc tcgcgaacga cgaggcgccg
     6661 cgggcgcccg cgccgccgag ctacttctga tcgtgcaaca ggccctcgta ggtgaagacc
     6721 ggcacccacg cgatgcggtc gcccacctgc ttggtctcca tcatgtagta gttgccggtg
     6781 cccacgaaca tgtcctggta cgcgctcatc ttgtaggggc cggccggcgt atcgatggtc
     6841 acgctcgaca tggcctcctt gagcttgtcc ggggaggtgt cgccgccgtt ggcctcgacg
     6901 gcgccggcga acaggttcac ggccagccag gcgccgaacg actgcatggt cgggtactcg
     6961 ccgtcctcct gcgccttgta ggcctcgacg aactccttgt tcacggggtt gtcgatctcc
     7021 ggggtgtaga actcgccgcc gatcaggccg actcccacct ctccgaggtc cctgagctgc
     7081 ggctcggtga ggttgctggc catggccgtg atgagcgggg cctcgaggcc gtagtcgttg
     7141 tactgcttga cgaagggcgc agcgccgttg ccgaagatcc accagtacgt gcagtcggcg
     7201 tccttgagcg tcgtgaggta ggagctgaag tcgaccgcgt cgatcggcac gaactgcgtg
     7261 ctcaggatct tgccgccggc ctcttcgaag ccgtggttga acccctcctg gagctggcgg
     7321 ccggccgtgt cctcgtaatt gatgcagttg gccgtcttcc agcccatctc ctccgccgcg
     7381 tacttgccgg tctggtagcc ggcgaacgag aacaggccgc tggggacgaa cgagaggccg
     7441 ttggcggtct tcatgttgtc gatcggctgc ccgtagatgg agatcgacgg gatgccgccg
     7501 ccgctcttgg ccagatagtc ggtgaccgcc gcgttcgccg gtgagaagat cgggccgatc
     7561 atgacgtgga taccgtcgtt ctcgacgagc tggcgggcct tgtccactgc ctggacgggg
     7621 tcggaaccgt tgtcggcctt gatgtactcg acgggccggc ccgcgatctg tcccccgagc
     7681 tggtcgatgg cgagcttgat gcccttgtcg gtcatcgtcg catcgaccgc catgaacccg
     7741 gtgaagccct catcgaaccc gatcttgatc ggctcgccgg tcgctgcggc ggcgggctcg
     7801 gcggattcgg tgctgcggga gtcggtcgtc tccgacccac aagccgccag aacgagggcg
     7861 ccgaaggctc cgagaacgag tacgagcgcc aggacggcga acacgacttt cctctgcatt
     7921 gtcagtcccc ctgagaagat gatcggaaag tagtcggtgt gctgcgctgc cgaaacggat
     7981 gcgacgcgcc ggcgctcctg gggtccctcc gccgtgaccg cccgtccgct gcgacgggag
     8041 acggcgaccc tgctgccggt cgcgaagctg aggtgctgcg ggtccctgct cctttcgtcg
     8101 gccctcgccg ctgtgaggac gagcctcccc tgaaggccca ttgtccagaa cggccagttc
     8161 tctgcgtccc cgtagtgcaa agggtaaggg tgcaaggtac cgcacgcggc cgcacgctgc
     8221 aagtgccacg aggcgaagcg gactactcgt gggtcgtcac gttgagcagg gtcgcccgtg
     8281 cagagatcgg cgtcgtggcc agagctccga agtcgctgcc ctcgtacccg aacagcctgc
     8341 cgttggagcg cttggctctc cagtccacca ggaccacgcc gagcgtcggg atgaccttct
     8401 cgcgccacac gaagaggtag agttcctcgc cgaccttgcg gtagtgacag cggtcggtgt
     8461 cggcgagtcc ccgctccgcc ccgcgcaggc actgccacgt gtagaggtcc cggctcaggt
     8521 agatgtgctc gtacgcctcg gtctggctgt agacgtactg cacgcgcttg cccacgaggt
     8581 cgtccgtggg cacgtgcggg tgcgccccgg gcacgaacgg accgtcgatg acggccggca
     8641 cgatgccggc ggcgacgagc gtgagctccg tccctcccgt cgccagcgcg aacgccccgc
     8701 gcgcggcctg gtccgccgtc ggcagggtgc cgacgacggt ggtcgccgcg ctccgcgcca
     8761 agtcgagcac gatggtcacg gccgtggctc gccgggtgct ggccacgtag tcgacgacgg
     8821 ccacctcctc gcgaggccgc gtgatgtggc aggtctcctc gcccgtctca cccgcgcccg
     8881 gcccggcggt gacctcccag gagagccgac cgccccgcgc gaacgacagc gccgtgaccg
     8941 tcgcgtcctc ggcgtgaagg ctcagtgacc tgccggcgag ggcgtcgtcg gccggcaggg
     9001 tgccctcgct gatgtccttg acgagctcac cgacctgaat gaagcgaagg gcctcgtccg
     9061 ccatgagcgc ctcccttgtc agatcacgct ctctctgcga cgcgccgagg tcagtacccc
     9121 ttgaggatgt cgatcattat ctccgtggcc ctgaccgcgt agtcgcgcgg cacggtgagc
     9181 ggcggcatga agcggaacac gttcgcgtgc cgcccgcatg gcgtcatgag gatgccgctg
     9241 ttgagcagct tgaaggcgat ctcggcgacc ttctcgccgg ccagcggctc cttggtgtcg
     9301 cggtcctcga ccacttcgat gccgaccatc aggccgttgc cgcgcacgtc gccgatgacg
     9361 ggcacgttcg gcatggcgtc gacgaacatc cccttgatct cctcgcccag gacggcggcg
     9421 cgaccgatca ggtcggtggc gggatcggtg aggatgtcga tgttggtcat cgcgacctcg
     9481 caccccatcg cgttgccggc gaaggtgctc ggctgcgagc cctcacgcag cgccccttcc
     9541 atctcggagc gataggtgac tccggccatg ggcatgtcgc cgcccatgcc cttgccccac
     9601 gtgagcatgt cggggacgac cttggagtgc tggacggccc acatcctgcc ggtgcggccg
     9661 gcgcccgact gcacttcgtc ggcgatgtac aggcacccgt tcttctcgca ggccgccttg
     9721 atcatctcca ggaactccgg cgggggcgcg atgtagcccc cctcgccctg ctgcgactcg
     9781 atgaccacgg cggcgacgtc gtcggcgccc gtgtacgggc cgttgaggat gtcgtccacg
     9841 tacttgccgc actggacgtc gcagcccggg tactccaggc cgaacgggca gcggtagcag
     9901 tacgcgtacg gcaggtgctg gacgccggcg atcagcgggc cccagccgtg gcggtagttg
     9961 taggacgtgg tcatggccgc ggtgccgcac cagacgccgt ggtaggcgcc gtggaaggcg
    10021 atgagctgag agcggccggt gtaggcgcgg gcgaacttga tcgccgtctc gacggcgtcg
    10081 ctgcctgcct gatagacggc cgtgtggcac tcgccggcaa gaccgtcggg catgatgccc
    10141 gagaccttcc tggcaagctc gatgcgcttg gggttggtga tgtccgtggt gtgcatgatg
    10201 acgtcgcact ggcgccggat ggccgcgagg accttggggt ggttgcgtcc cacggcgttg
    10261 accgccacgc ccgcggccat gtcgatgtac atgttgccgt cggcgtcctt gacggtcacg
    10321 cccttgccct gctcgaggat gacagggaag ccgccgccgc cgcgagtgaa cgattcgtac
    10381 ctggccgact cctccatcag tgcgagcacc ttgggacccg gcgcctcggt caccatgctc
    10441 ggcgcatccg gccaggacag ctccatcagg tgtgcttcga gtgcttcagc gttcatgggc
    10501 gtcacctcgt tcacatcgag tctgcgaccg atccgctcgt cgcggcagag tcgctacatg
    10561 atactgcaat accacatgac gacaaggaga ctacgcgtac tgcggcatgc gtggccgcgg
    10621 tagttcggag aacgacgcag ccgcgcaaac ggcgctggtg caggaggtcg atggtcgcga
    10681 tgatctgccc ggtctgactg cgcactccgg atgtcagatc gcggccaggg cctctgcaac
    10741 gaggacgggt gtctcgtctg gacggcgggc gaccgggatg cccgccgcct cgagtgcagc
    10801 ggccttggcg gccgccgtgc ccgacgaccc cgagatgatc gcgccggcat gacccatgcg
    10861 cttgccgggc ggagccgtga aaccggcgat gtaggcgacc agcggcttgg tgaggtgctc
    10921 ggctgcgaag ccggcggcgc gttcttcgtc gtcgccgccg atctcgccca ccagcaccac
    10981 ggcctcggtc tgcgtgtccg cctcgaagag ttcgaggcac tcccggaagc caagcccatg
    11041 gatggggtcc ccgcccatgc ccacgcaggt cgactgcccc agacctgcgc gcgtgaggcc
    11101 catggcgatc tcgtacgtca gggtcccgct gcgggagacg atacctacgc ggccgggcct
    11161 gaacagcgac gccgccatga tcccggcatt gcacccgtcc ggcgtgacga tgcccgggca
    11221 gttgggaccg atgaggacga agggccgctc ggcgactctg ccatcgtcca gcttgagctc
    11281 cgcgacggcc tcgctcatgt cgcggacggg gatgccctcc gtgatgagca cggcgacacg
    11341 gacgccgccc tcggcggcct ggacgacggc cgccttcgcg aagcgggccg gtacgaagat
    11401 gacgctcacg tccgcaccgg tggcctggac ggcctcgccc acggtgtcga agaccggcag
    11461 tccgcaggcg gactggccgc ccttgcccgg ggtgacgccg gcgaccacag aagtgccggc
    11521 ggcgagcata cgctcggtgt ggaaggtgcc ttctcggcca gtcatgcctt gcacaaggac
    11581 ccggctggac ttgtcgacga ggatgctcat gcccggctcc cggtcttcgc cgctgcgacg
    11641 acggactcga cggcctcctg ggcgctcagc gtgacgctca ggccgtcgat ccccgccgcg
    11701 gcgatcagct gcagtccctc cgcggcgttg ttgccgtcga ggcgcacgac gagcgggaga
    11761 tcgacgccgg tcgtctcgag ccaggcgagc acgccgcggg cgacctcgtc cccgcgcgtg
    11821 ataccgccga agatgttgac gagcagcgcc cggacgcggt cgtcggacgt gatgatgtcg
    11881 agagcggcgg cgatgcgctc tgcgcgcgcg ccgccgccga catcgcagaa gtccgccgcg
    11941 gcgccaccgg cggcgccgat catgtcgagc gtgctcatga cgagaccggc gccgttgccc
    12001 agcacgccca cgtcgccgtc gagcgtgaga taggtgacgc cggcctcccg agcggcgcgc
    12061 tcccgggcgt cctcgtccgg ccgctgcgcg gcgaggtcgg gctgacggta cagcgcgttg
    12121 tcgtcgatgg tcaccttggc gtcgagagcg acgagtcggg cgggatcgcg aacgaccgcc
    12181 agcgggttga cctcgaccag cgatgcgtcg cgctcgcggt agagcgtcca cagacggtcc
    12241 acgaggtcgc cgagctgctc gtccaactgc gtatcgccga gcgccgcggc ggcgacgacc
    12301 gcgtcgcgcg gcccgccgca gtccggcgac acagcatcga gcggcagccg cagcaacgcg
    12361 gcgggatcct gcttcgccac cagctcgatg tcgacgccgc cgcgcgccga gaagatgagc
    12421 agcggcgcct tggcgtggcg gtcaagggtg acggccagat acagctcgcg catcaccgaa
    12481 gcgccctgct ccagcagcac gctgtggacc ggcttgtcct tgatgatcag ccggaggatc
    12541 gccgagacgg cctcgccgag ctcctgcggc cgcgtgacca cgcgcacgcc gccggccttg
    12601 ccccggccgc ccgtgagcac ctgagccttc acggcgagcg ggtagccgag ctctgccgcc
    12661 gcgcgctcgg cttcctcctc ggtgaagacg acccgcgacg gaagcacggg gatgcccgcg
    12721 gccgcgaaga gctccttacc ctggtgttcg aacaggtcca ctggtctacc tcatcgcctg
    12781 gcggcctcgg cccttactgc ccggtgccga agcggaaggt ctgatccggg tcgatcacgg
    12841 ccagcacccg caccatgcag ccgtcgccgc ccggccagcg ccacggcagc gcgagaaacg
    12901 tgcagcgctt gccggtgacc tcgtccaggt cgccgccgac gttctcgatg cccgggatgc
    12961 cgccctcgca catgaggatc ttgtgcgccg gctcccagct ggggaagtcc gccttggcgt
    13021 cgcggccggt gaactccttg tactcgtcga tgaggtgtgg ctgtgtcggg ccgagaccgt
    13081 ggtcgacgag cttggtggcg atggggtgat cgttggcctg gtggccgtag cccaccagct
    13141 tgacctttct gtcgaccagc caccgggcgc cctcggcgcc catgccgcag ccgtaggcga
    13201 agtactcgtc ggtgtctccc cacagacggt ggaagcccgt gttgatcatg acgatgtcgc
    13261 cctcttcgat cttcggcgtc gcgttctcca ggtcctcggg cgtgatgacg ccccacttgc
    13321 ccttggggat gtcgacgcag acgcccgtac cgacgagccg ccagagcgga tagtcggcga
    13381 tgctcggcgt gttctcggtg acgtggagcg gggcgtccat gtgggtgcag cggtgcatga
    13441 tgccctccca ctccacctcg tagacgccgt ccttggcatg gaacttgctc accttgacgt
    13501 tgacgctcgg cgtggacggc cactccggga tgaactgggt gaacgtgtgg ctcaggttgt
    13561 agaccttcag actcatgtcc ggttcccttc tcgtccgcgg atcgtcagtc gccggcgtcg
    13621 atacgcaggt cgccggacgg gtcgagcatg gcgatcatcc gcaccgggca agcgtctccg
    13681 tgctcgaact tccagggagt cgccgcgacg gtggcgcgct tgcccttgac gaggtcgacg
    13741 tcgccgccca cctgctcgac cgtcgggatg ccggcggcgg ccagggtctt gtgggcgggg
    13801 aaccagtccg gaaagtcctc tttggcgtcc cggccggtcg ccgccgagta ctcgacggcg
    13861 aggcgcttca tctgcggccc gccgcggtgc ggccccatgg aggtggcgag cggcatatcg
    13921 atgaacggcg tgtccacggc cacgagcttc gggtccttct tgatgagcca ttcggccgcg
    13981 tcccgggtga ggccgggagc ctcgccgaag tactcgagac cgtccgagta gttgtgatgc
    14041 cagccggtga cgatgacgac gatgtcgccg tcctgcaccg ccggcgtggc cgcctcgagg
    14101 tcggcggccg tgacggcctc ccagctcttc ctggggatat cgagcacggc ggcattgccg
    14161 aacaggcggt ccggggagac ggccgccagg tcgtcgccgc gctggaccat gtggatgggc
    14221 gcgttcatgt gcgtgcccgt gtgcaggctg gtggtgacgc gccaggcgag cacgccgtgc
    14281 tgggcgtgct tgacggcgcg caccatgcgc acgtccagct gtcccggctc ggacgggacg
    14341 ccgtggcccc acacgtggct cagctcgatg agctgcaatc ccgagtaggg atcggtgacc
    14401 acatctgtca tctctctctc cttcatggtg gtcagcccgg cggacctgcc ggctacccga
    14461 cggcctcgac gatcgtggcc atgccctggc cgcccgcgat gcagatgccg gcgagcccgt
    14521 accgcgtgcc acgccggcgc atctcgtaaa gcagcgtcgt caggatacgg gcgcccgagc
    14581 agcccaacgg gtggccgagc gcgatggcgc cgccgttgac attgacgatc tcctggccga
    14641 gctccagctc cttgaccacg gcgaggctct gcgccgcgaa ggcctcgttg acctcgatca
    14701 ggccgacgtc cgacagcgcg atcccggcgc gctggagggc cttgcgcgca gcgggcgccg
    14761 ggccgatgcc catgatgcgc gggtccacag ccgcggtggc ctgcgcacgg atggtcgcca
    14821 tgggcctgat gcggcgggcc ttggccgtct ccgcatccat gagcacgaga cacgcgccac
    14881 cgtcgttgcg tcccgaggag ttgcccgcgg tgacgctgcc gccctgcttg aagacaggct
    14941 tgagcttcgc cagctgctcg aggctcgtct tgcgtgggtg ctcgtcggtg tcgaacacga
    15001 cggactcgcc cttgcgctgc ggtaccacga cagggacgat ctcatccttg aagcggccgc
    15061 tcgcgatggc gtcggcagcg cgctcctgac tctgcagcgc gaacgcgtcc tgctcctcgc
    15121 gcgagatgtc gtacttctcg gcgaggttct ccgccgtgag gcccatggtg aggtcgccgt
    15181 agacctcgta gggctgggag cgcggctggc tctccgtgtt ggagtcgacc acgacggtgt
    15241 tgccggaggt gaagccgaag cgggcgccgc gcaagtagta cggcgccagg ctcatgctct
    15301 cgacgccgcc ggcaacgacg atgtcggctt cttcggcgcg gatgacctgg actgcgttgt
    15361 ggatggcctg cagcccggag ccgcactggc gcatgaccgt gtaggccggc acctccaccg
    15421 ggaagccggc cttgagcgcc gccacccgcg cgaggttggc ggcgtcggcg ctctgcttgg
    15481 tctggccgat gatgacctcg tcgaccgacg gcggctccac cccggcacgc tcgacggcgg
    15541 cgaggatcac gatcttcgcg agctcctcgg gctggacgct cttgatggcg cctccgacgc
    15601 gcccgagggc ggtgcgggcg gcagagacga tgacgacgtc acgcatgggc gaccaccatc
    15661 ctcagacgaa ggacatcgtg ccgccgtcca cgtacaggac ggtgccggtg atgaagtcgg
    15721 aggccgcgga ggcgaggaag accgaggggc ccacgaggtc gtcaacgtgg gccattcgcc
    15781 ccatgggcac gttgagcagc atgcgctcgt agcgcgccgc ctcgttgctg atgcgctcgg
    15841 ccgtcatcgg cgtcaccgtg atgatggggc cgagcgcgtt gacgaagacc ttgtgctgcg
    15901 caagctcgca ggcgagctgc ttggtgatca tgtcgacggc gcccttgctc gcgcagtagg
    15961 cgctgttgcc gccgagcgcg ccgcgctggc cgcgcaccga cgagaggttg atgatcttgc
    16021 ccccgccctg cttgaccatc tgggcgccga agtgcttgca ggacatcatg acgcccttga
    16081 cgttgacgct gaagagcttg tcccactcgt ccatcgggaa ctcggtggcc gcgcccttga
    16141 cgttgtaccc gagcgcgttc acgagcacgt ccacgcggcc acccatgtcg gccaccgtgc
    16201 tgtcgaccac acccttgacg ctggcctcgt ccgtacagtc gatgacgtag ctgcgcaccg
    16261 gcttgccggt cgcttccacg agggccgcgg cgacctgctc gagcgcctcc gggttgacgt
    16321 ccgccagcgc gacgtccgcg ccgtagcgga tgagtcccgc ggcgatggcc cggcccatgc
    16381 cgcccgcgcc gcccacgacg acggcggact tgccctcgag tgaaaagaga tcctccattg
    16441 cccggtcccc ttccctcagt acttcttgac gacgccgagg atctcgcgag cctcgtcggg
    16501 cgtggcaggc tcgagattga gctccctggc gatgcggacg gtccgttcga caagctgcgc
    16561 gttgccctcg gcaagcacgc ccttcttgta gtagatgttg tcctcaagcc cgacgcgcgc
    16621 gcagccgccc atgatcatgc ccatggtggt gatgggaagc tcggcggcgc cggtggccgt
    16681 cgtgttgaaa taggcgtcct gcggcatgaa ctggtacatc gagtacaggg tgtcggggtt
    16741 cgcctcgatc gcgccctgat gcatcatgcc gagcacgaag ttgatgtagt acggcggctc
    16801 gatcaggtcc ttcttgatga gattgtgcac gtcccggtac atgctctgcg agtagcactc
    16861 catctcgggc ttgatgtgca tctctttcat cttggccgcc cacgcctcga tgtctttggt
    16921 catgttcgag aagggctctc cggcgttcgg acccgtctga cgcatcagcg ttcccatgtt
    16981 gagcgaggcc atctccgggt cggcgtccag acacgagacg cgctcctcga tggagaggtt
    17041 ggtcccgccg ccggtcgaga actggacgat gacttccctg cactcggcct tgacggcagc
    17101 ctggatctcg gcgaacttct ccttcgtgcc ggcgggcttg ccctgctcgt cacgggcgtg
    17161 gatgtggacg atcgcagcgc cggcgtcgta gcactcacgg gaggcgcgcg cgatctcgtc
    17221 cggctgctcc ggcacgttgg ggttcatggc cttgttgacg aacgcgccgt tgagcgacgc
    17281 ggtgatgatg actttgcctc gcgggagctt catggtccat ccctccactt cttgccgggt
    17341 gagccggccg gtctccgtga cttcgcacgg cgcgcaggcc ggtctcagac gcgcttgtag
    17401 atcaggtcct tcgactggtt gaagacctcg atgagggcat cgtcgcgggt cctgaggacc
    17461 tcacgcaggt ctctgtcgct gtagtcgaag aaacccttac cgctcttgac gcccagctcg
    17521 cccttctcga cgtgcccgac gaggctgctc ggcgcattgt cgatcggcgg ctcgaggaag
    17581 tccgagttct gcaggttgtt cagactcagg tcgagacccg tgaagtcata ccgctgcacg
    17641 aatcccagca ccatcgcccg cgggatgatg ctcgccttca ccgcaaggtc gagttgttcg
    17701 gcagtcatgt agccgttgtc gaggaggaag aagacctcgc ggccgattat gcgcaggaac
    17761 cggttgatgc agaaacccgg gacgaacttc tccatgacca cgggcacgcg gtcgaccttc
    17821 ttgagcagtg cgacgacgag ctcgaccgtc tcgtccgagg tcgactcacc cttgacgacc
    17881 tcaacgagcg ggatgatctg cggcggcgcg aaccagtgag cgatgacggt gtcgggcaga
    17941 cgccgctcgg ggaccacctg gtagatgtcg aggtacgacg tgttgctcgt gaagatggcg
    18001 ccgtccggac acgcccggtc caggtcggcg aacaccgcgc gcttcgcctc caggttctcg
    18061 gcgatgctct cgatgacgag gtcggcgccg gcgccggcct cttcgaccga ctgcgtcgtg
    18121 gtgatacgag ccagcgctgc cgacacgtcg tcgcggctga ggaggccgtg ccccgcgaac
    18181 gtctgcatgt tggcggcggt gacggacatc gccttttcga gcgtcgccgc ggagcgtgag
    18241 tacagtgaga ccgggtgtcc cgcacaggcg aacacctggg caagacctgg tcccatggtg
    18301 ccggccccga cgactgctac cttcttgacg tccatgacat ccactgctgc ggctcctcac
    18361 gttcgacgcc gaccgtccac tggcgcggtg aggagtctcc cccgcgttgg aggaagtccg
    18421 cggtcaagag gcgacccgcc acgctaccgc acaatggtat cgccatatca caatatcagc
    18481 gagcgcggac tgtagcaaca tacgcgggtc atcacgcgga cctcctgcat gcgaggtcct
    18541 ttgcgcgggt cgcgtggatc cggcggccgc gcgtgacgag ccggtgtcag actcgcgcgt
    18601 cggccccggc gtagcccagg aaccccgaga gcgccgcggc ggacgccttc accgcgctca
    18661 cgcaggtctc gcgctgctct gtgccgaagc ggccggtcgg cacgatgacg ccgatgctgc
    18721 cgatgacgtg acccatctgg tcccggacgg gcgcggccac ggcgcacgtg cccaggttgc
    18781 gctcttcgat gtcgaaggcg acgccctcct cgcgcacccg tcgcagctcc gcggcgagct
    18841 cctccggatc ggtgatggtc cggggcgtga gccgctcgtg cggcagcgag agcaggcgct
    18901 cgcgttcgcc gtccggcatc gacgccgcgt acatcttgcc gtgtgcattg gcggtgtcgc
    18961 cgatgacgcg gccgacggcg acctcacgct tgtgcggccg cggggagtcg agcatgtcga
    19021 cgcacacggc cacgccgtcg acgtccacgg cgagcgtcgc ggactcgccc gtctcctcga
    19081 cgagcgcctg caggtacggg cgagcgatgg ccaccagctc ggcgtagccc tccgagaggt
    19141 acgtcgcggc gagaagggcc gggccgagac ggtagcgccc gctctgcgtg tccgtgacga
    19201 gatagccggc gctctgcagg gtccgggcca tgcggtagcc ggtcatgcgc gggagctgga
    19261 gctccgagac catctcgtcg agggtccact cgcgatgctc ggcgtcgaag agaccgagca
    19321 tcgccagccc tttcgccaag gcgcggacga cgaacgcctc tgcgcccgcc tccagcggtc
    19381 cgttggcggc cggctgccgc ttcttcttcc cagccttgcg cgtctctttg gccatggccc
    19441 ctgtcttctt tctctcgtgg atcagctcgt gaacatcgcg tccaggtcgg cctcggcgac
    19501 gtcgtaccgg gtaccggacg gcaggaccgc ctcctccgtg atgaacgccg gagggcggtc
    19561 gtccttggcc gtgaagccgg cgcggcggtt gaagtcgcgc tccccgcgta cgatctggtc
    19621 gcccatcctg atgaggtagt cctcggggag cgcctcgccg gtgatggccg ccgcagcctt
    19681 gacgaactcc ccctgcagct ccggcatgtc gagcgcgggg agtgtcgcga agaggcacat
    19741 gcccagcgag tcgatcgcgg cgaagtagca ctgcaggaac tcggagatct gtgcctgccc
    19801 ctcggcggaa gcgggatcgt aggtcggatt ggccggactg ggcagggcgt tgcccgcggt
    19861 gtggtcggcg ccctgcgggc tggtgatgta ggtggtgccg gtgcccttga gcacgcgcgg
    19921 gtcgtaggcc gcgaagctct ggcctttgac cgcgggaacg cgggcgacgc cgaggcgctg
    19981 cccggtcgtg acgcagccgt cggcgagcat gcgcgcgtcg gcgtcgccgt ccctgagctt
    20041 gctgagcagc gcgaacgccc cttcgccgtc gccccagtcg agcaggccgc cctccatggc
    20101 gacgcccatg gcggcgccga cttccatcgt gtcgagcccg aggtcgtcgc agaggcgatc
    20161 gagccgtgcg atctggtcga ggtccttgat gtggcagttc gagcccatca gcccgagcgt
    20221 ctcgaactcg acgccggagg tgatgacctc gccggcgtcg tccgtgtaca cctgcgagca
    20281 gttgatgatg cagcccttca tgcagctgtg cttggtggcc ccattgggac gcgcggagat
    20341 gatctcgacc atgtgctcgc cgctgatgtc cggcgcgttc tcccaggtcc cggcggagaa
    20401 gttcctggtg gccaggcagc ccatggactg cgtgacggtg acgaggaacg ccgagccgag
    20461 cgcctcgagg gcggccatgg ccggatgcga gtggatgccg gtggcgagag ccttggaggc
    20521 ctccttgagg gcgtccttgt cggcgacgac gacgcccggc ccccctgcgg cgtcgacgac
    20581 gatcgccttg aggttcttgg agcccatcac ggcgcccaga ccgccgcgtg cagccgcgcg
    20641 catgtgatgg tcgggcgtgg tgacgaggac cgcggcggcc ttggcgccca tctcccccgc
    20701 cgggccggtg cagatgagcg agaccttgtc gccgtactgc tcccagaggc gctccgcggt
    20761 ggcgaacgaa ccgaggccgg cgagctccgg cgccgggacg agcgtgccgc cgcccgccgt
    20821 gaccaccagg accgacgtct cgtcggcctg ccccgtcacc ttgagggcgc gcaggccgag
    20881 ctcggccagc gcacgcgcgg ccgagccgcc ggaatttgcc tccttgatgc ctccggtcag
    20941 cgggctcttg ccccccacgg agagacgccc gccgttgggg acgacggtgc ccgcgaagag
    21001 tccggcggca tacaccagga cgttgccggg tccgagggga tcggcctcgg gatggacttc
    21061 accgcagacg atggccgagg tcaggccatg gccacccagc ggctcgaggt ccgcacccag
    21121 cggctccctc gccaccgcgc cactcgtcag gtcgacgttg aggaggtcgg tcacggtcgc
    21181 tcacatcctt tcccgggcac ggagcgtctc gctcgctcgt cctcccgcgc cccggtgctg
    21241 accacgaagt gatatgtata tatcactcca acgacctgag cattatgcgg ccagacgctc
    21301 gcgcatgcaa tagagccgcg cgcgtggacg cctcacccca tcggctcgaa gcgcgcctgg
    21361 aagaagcgca gatcgctggg ctcgtagacc atcgtcaggc ccttgaccgc gccgcgctgg
    21421 tcccagacct ccgccacgga ctccgccacg acgtccatgt gcgcctgcgt gtacacgcgc
    21481 cgtgggacgg tgaggcgcac gagctcgagg gcaggacgcc ggttctgacc cgtctgcggg
    21541 tccctgccgg cgctgacgtt gccgcgctcc atggcgcgca cgccgctgtc caggtagagc
    21601 tcggcggcca gtgcctgcgc cggatacagg tcctggtcga ggtgcggcag gatcgccctg
    21661 gcgtccagga acacgccgtg actgccgatg ggcacgacga tcggcacgcc gtagtcctgc
    21721 agcttctcgc cgaggtactt gacctggccg acgcgcgcct tgatgtggtc gaagtcgacg
    21781 ctctcgcgca gaccgatggc gaccgcctcc atgtcgcggc cggccatgcc gccgtacgtg
    21841 tgcaggccct cgtagacgac gaccaggttg cggcacgcct cgaagacctc ccactcgttg
    21901 agcgccagga agccgccgat gttgaccagg gcgtccttct tggcgctcat cgtgcagccg
    21961 tcggtgagcc cgcagatctc cctgacgatc tccgcgaccg tcctgtccgc gtagcccggc
    22021 tcgcgttcct tgatgaagta ggcgttctcg gcgacgcgcg tggcgtcgtg gatgatgcgg
    22081 atgccgtggt cgctgcagag cgcgcgtacc tggcgcaggt tggcgaggct gatcggctgg
    22141 ccgcccgcca tgttcacggc ggtggcgatg cacacgtaag ggatgcgctc ggcgcccacc
    22201 tcgtcgatga ccctctgcag cttggcgagg tcgacgtcgc ctttgaaggg gtgctcgctg
    22261 gtggggtcgt gcgcttcgtc gatgatgacg tcgacgaagc gcccgcctgc cagctcttgg
    22321 tggaggcggg tcgtcgtgaa gtacatgttg ccggggatca cgtcgccctg cttgatgagc
    22381 gtctggctga gcaggtgctc ggcgccgcgg ccctggtgcg tcggcaccag gtacttgtag
    22441 ccgtagacgt cctggactga cgattcgagg tggtagaagt tgcggctgcc ggcataggcc
    22501 tcgtcgccga gcatcatgcc ggcccactgg cgatcgctca tcgcggacgt gccggagtcg
    22561 gtgagcaggt cgatgtacac gtcctcgctg cgcagaagga acgtgttgta gccggcctca
    22621 cggatggcgg cctcgcgctc ctcgcgcgtc gtcatcttga ggggctccac catcttgatc
    22681 ttgaagggct ccgcccagct gcggcggcgt ggctcggtca tcaggtcctc catcactgtc
    22741 gtccacggcc ggcggaggcc ggccggaagg tcacgtggcc tggcccacca ggcggtcgag
    22801 ctcgcgcagc gcgtcgtcgc cgggcgtgta ggcgcgctcg gcgtcgcgca gctcgcgggt
    22861 acgctcggcc gctcgctcga ggaaggaagc ggcgcctgcg gcccgccagg actcgagaga
    22921 gtcctgggag atcaggcgcg gccggaagaa gtcccggacg tgccgtgagt acttgcggcc
    22981 gaggtgcgtg ccgcctggtc cggccgcagc gatctcggcc gcggccaggg actcctcatc
    23041 gatggtcacg ccggcgacat aggccttgac ccagcccgcc agttcgtccg cgacgacgat
    23101 ggactcatac gacgaggccg tgcccgaggc gacgtacccg aggtcgtgca ggagcgtgac
    23161 gcccgaagac gccgcggtga gcagcgtcat gccggcctcg agcgcccact gctcgtccag
    23221 caggagggag tccgagcagc cgccggtccc gaacgagggc agcccgtagt gggcggcgag
    23281 gtcggccgag gcctgctgta ccgccatctc ctccgctccg cagtacacga tgtgaccggt
    23341 gcgcggttcc atccatccct gtgaggtgcc gtagacgaag ggcgctcccg ggctcacgag
    23401 ctcgctgatg acgaggccgc tgagcacctc cgcattggcg agcagcacac aggccgcggc
    23461 agacgccggc gccgtggcgc cgggcagata cgcgccggcg tagaccatgg ggatgccgag
    23521 ccgtgcgcat ccggcgagcc tgtccaccga ctctctgccg tgcgtcagcg gcggagtcgg
    23581 catcgcgtag atgccccaac tgcccgctcc cccgcacgtg gccgccatct cgttgaacag
    23641 cgcgaggtgc cgggcatcct cggggaccat gatcagcggc ttggacgtgc cgcgaagcat
    23701 cgccgcgaac tgcgcgaccg gtgcgacgga agcgggcaac tcgtgagggt ggaccatcga
    23761 gagcacgaag tcgatgttgg gcagcttctc ctggagggcc gccatctcct ccacgtcggc
    23821 cagggtaccg tcacggcggt cgcgtggccc cggcccgagc acgaagatgc agtcggagcc
    23881 ggtgccgtag tagacgggtc cggacgtgag gtcgagcccg gacgtcacgc cgcgcgacgc
    23941 gagggtgatg ctgcgcggcg cggcggccag cgcgtcgtcg acgaggccgg cgggcatgca
    24001 cacgcgtgat ccctcgacgt tggccccggc ggccttcagg agcgacagcg cgtcctcgtg
    24061 ctgtacctcg acgccggtct tctcgagcac ctcgagcgtc gccccgtgga ccgcgtggca
    24121 gccctcggcg tccagggcct tggtgtggag ccggaactgt ggcatctatg tcctccccgt
    24181 cgcgcatcac ggccgctgcg ggaccccgcg gcacaggcca cactttccat caggagtgga
    24241 cggaagtcaa gtaatagagg atactgctgc acaggtcggg tctttgacgc ccgccacggc
    24301 gctccgctac agtcgaggcc catgtcccag ccgcccgcca gagacgatcg ctcccgcgcc
    24361 gatgaacggt ggccggccag gatggtgggc gggcggctgc gcgaggcccg ccggagccgc
    24421 ggcatgacgc tcgacgacgt cgcccgcgag gcgggcctca cgaagtcgtt cgtgagcgcg
    24481 gtagaacgtg gggacacgtc gccctcgatc ggctcgctct accgcatctg cgaggtgctc
    24541 ggcatccccg tcgcatcgct gttcgagacc gcgccggcgc cggagagcaa cgtcgtccgg
    24601 cacaccgacg tgcgcggcgt ctcgttcggc ggcgagggcg tggagaacta cgtcctctcg
    24661 cctcgtgcgg agcgccgcgc ccaggtgatc gagacccacg tcgcgccggg cgggtcgccc
    24721 ggccccgagc cctggtccca ctcgggcgac ctgatcatcg cgacggtcct cgagggcacg
    24781 ctcgagttcc gcttcgagga ccacacgacg gcgctccacg caggcgacac gatctcgtac
    24841 tcgccggccg agccgcactc gtggggcaac ccgagcgacg acgcgccggc cgtcgcgctg
    24901 ttcttcggca tgccggcgga gtactgaggc tccgcggccc agcgtcgaca gaggcgcacc
    24961 tgccgcgcgt gcccgagcgg cctgagggta agctccccca cagatgaaga cgttctggcg
    25021 catcctcaag ctcctcggcg actacaagtg gctcaccatc ctgggcttcg ccttggcctt
    25081 cacccagatg ggcctcggat tggtcgtgcc gcgcatcatc cagctcacga tcaacaacgc
    25141 gctgatcggg ggcgagaccg gccttctggt cacctacggt ctcgccctct tcggcatcgc
    25201 cgtcgtccgc ctcttcgtcg gcgtcggtcg caggctcgcc agcggcaagg ccagcctcgg
    25261 catcgagtac gacctgcgca accgcatgtg gacgcacatc ctgcgccagt cgttcagtta
    25321 cttcgaccgt tggcccaccg gccagctcat gagccgggcg atgagcgacg tccagaacgt
    25381 gcgcatgttc ctgggctacg gtctcatctt cttcgtcacc aacatcgtga tgatggtggc
    25441 catcgccgcg atcctcctcg tcatggagtg gaagctggcg ctcatgtcgc tggcgttcct
    25501 gccgctgctg ctgctggtga cggtccgctt cagtctccgt ctcaagccga tcctcgtcga
    25561 cgtgcagcag aagatcgccg acgtcacggc tgcggcggag gagaacgtgg tcggcagccg
    25621 catcgtgcgc atcttcgcgc gcgaggacga cgagctcgtg aagttcagcg accgcagcta
    25681 caaggtgttc gaggcgagcg tggccgccgc caggacgcga gccgtctaca tcccgctcat
    25741 cgccttcatc cccaacctcg ccgtggcctt cctgctctac tacggcgggc gtcaggtgat
    25801 cgagggcgac ctgagtctcg gctcgctggt cgccttctac agctacctga tgatgctcgt
    25861 gtatccggcg caggtgatcg gctggctcac cggcctcgcg cagcgggcga tcgcgagcgg
    25921 cgagcgggtc tacgaagtgc tggactcgcc cctcgagatg acggagaagc cggatgcgca
    25981 gccgctgcgg gacgcgttcg ggctgaccgc cgcgcaggcg accggggatg gcgcggcggg
    26041 cggcgacggg acggccctcg ccggcgcggc cgccccggtc ggcggcgccg gcccctccgg
    26101 cgccgtcacc ttcgacgacg tctggttcag ctacgtggac cggccggtgc tgcgaggcgt
    26161 gagcctcgag gtcccggccg gccacaccat cgcgctcgtc ggccacaccg gctgcggcaa
    26221 gacgacgctc accaacctcg tgccgcgctt ctacgacacc ggcggagggc gcgtgctcat
    26281 cgacggccag gacgtacgcg acctgcagct cgccgacctg cgccggcaca tcggcatcgt
    26341 caaccaggac cccttcctct tcagcgcctc catcgccgag aacatccgct tcgggaggcc
    26401 ggaggcgaca gacgacgagg tccgcgcggc cgcccgtacg gcgcagaccg acgacttcat
    26461 cgaggacctc cccgaaagct acgagaccgt catcggggag cgcggcttca cgctcagcgg
    26521 cggccagcgc cagcgcatcg ccatcgcgcg ggcgctcgtc atggacccgc gcatcctcat
    26581 cctcgacgac gcgaccagca gtgtcgacgt ggagacggag ttcaggatcc gggccgcgct
    26641 gcaggaggtc atgcgcggcc gcaccacctt catcatcgcc catcgcccga gcaccatctc
    26701 gctcgccgag gaggtcgtgg tcctcgacca gggccagatc gtggagcgag gcagacacgt
    26761 ggagctcatg gccgccggcg gcgtctacgc gcacatgttc ggcgacgccg agcgcgaggg
    26821 ctgctgcctg gacgacgccg gcgaggagaa gaccggcccg gcggcctcgg ggggtgcggc
    26881 ctgatggcca ccacctcgcg acacagcgca cagcacggca ccggcgccag cctcgaagag
    26941 ctgaagggct ggcgcgcgtt cctgcacctg gcgagcgagc tcaagctctc gcggctcgcc
    27001 cgcctcgcct ccccgtaccg acggcgcgcc gccctgagca tcgtcgcgat ggtcatcgtc
    27061 acgctctcga gcctcgccgt gccctacctg ctcaaggtcg ccatcgacgg cggcatcacc
    27121 gccgggaacg tgaccgtcct cacctgggtc atcgtcgcct tcgtcgccgt cagcctcgtc
    27181 aacctcggcg ccagctacct gcagacgtac ctcaccagct gggtcgggtc gcacatcatc
    27241 tacgacctgc ggcggcagct cttcgcccac cttcagaagc tcagcctcga cttcttcagc
    27301 cggcagaaga ccggctggat cgtgagccgg ctgacgaacg acatcgacgc cctggaccag
    27361 ctcgtgacgg agggcgtcac gtcgctggtc accaactcgc tgacgttcat cggctccatc
    27421 gtgttcctct tcatcctcga ctggcgcctg gccctcgcca cgctcacggt gatgccgttt
    27481 ctcatcgtcg ccacgctcat cttccgcgcc aggtcggcgc gcgcctacgc ccaggtgcgc
    27541 aacaagatcg gcgacgtgag cgcccacctg caagagtcga tctcgggggt gcgcgtcctc
    27601 aaggccttcc gccgcgagga gagcgactac gagcggctca aggtggccaa cgccgcctac
    27661 cgcgaggtca acatgcgcac cgtcgtgcag agcggcgtgt acttcccgtt cgtggagttc
    27721 gtctcgacgt ccgccgtcgt catcgtcctc tggtacggcg gctccctcat cgggggcggc
    27781 agcctcgaga tcggcgtgct ggtcgccttc atcggctacc tgaacagctt cttcgacccc
    27841 ctgcagcagc tcagccagct ctacaacacc ttccaggcct cgatggccgc cgtccagaag
    27901 atctacaagg tgctcgacac cgaccccgac atgctggacg cgccgggcgc cgtggacctg
    27961 cccgacgcgc acggcgccgt ggagctccgc gacgtgacgt tcggctacga ccccgccaag
    28021 ccggtgctgc acgaggtgga cctccggatc ccggcgggga agaccgtcgc gctcgtcggc
    28081 gccaccggcg ccggcaagtc gacggtcatc aagctgctcg cccgcttcta cgatcctcag
    28141 cagggcgagg tgctcatcga cggtcacgac ctgcgcgagg tcacacagcg ctccctgcgc
    28201 gagcagctgg ccgtcgtgcc gcaggaggcg ttcctcttca gcggctccgt gctcgacaac
    28261 atccgcttcg cgcgcccgcg cgccagtgac gacgaggtca agcgcgtcgc ccgcatcgtc
    28321 ggtgtgcacg acttcgtcga gggcctcccg gacggctacg agacggaggt gcaggaggga
    28381 gggtcggccc tgagcaccgg gcagcgacag ctgatcagct tcgcccgggc gctgctcgcc
    28441 gaccccagga tcctgatcct ggacgaagcg accagcagcg tcgacgcgga gagcgagcgc
    28501 cgcatcgagc gcgccatgga ggtgctcttc agcgggcgca ccagcatcat cgtcgcccac
    28561 cgcctgagca cggtgcggta cgccgacgag atcgtcgtgg tcgacggcgg ccgcatcctg
    28621 gagcgcggta cgcacgacag cctcgtcgcc tccggcgggc gctacgccgg gctgtaccgc
    28681 gactgggaag cgaccggcct ggcgctttga gcgttcggcc gtgcgcgccg gatgggcggt
    28741 caggccacct gtgcgagcag gtccttcatg ctcgtgaacc cgccgtgcac ccagcgtgat
    28801 gacggcagct cagcgcgcca tccagagaat cctcgtcgag cgaccctgag agctccacct
    28861 ccccgtgtcc gcttcgggac ggccatggtc gatgggactt gaacgccctg cgagcgacgg
    28921 cagttccgca cggcgccgcg ccgtctctca accgtcgagg gacttggccg cgtgagcacc
    28981 ggctggctcg agcggtccca tgtccttagg ggcgcgcacg tccctccagg agacctggaa
    29041 ggcgctgacg agaagagcca cggtcgcgct cccccagagc acggtgtcga tgccgaaggc
    29101 gtccgcgagc ggcccggcca gcgcaagccc cgccgggtag aaggccagcg agccgaagta
    29161 gtcgtagctg gagacgcggg agatcatgcc gggggccacg acgcgctgca tgcacgtggt
    29221 ccagaacggg tcgtacaagc cccactcgac ccccatgacg gcgagcgaga cgtacagcgt
    29281 ccagaccggc gcctccatgg cgaggagtgc gaccggcacc gcggcgaggc tcatcccggc
    29341 ggcgatcacc agcatcgggc gccgcggccg ccagcggagg ctgagcaggc cgccggcgat
    29401 ctgcccgacg cccatcgccg ccgaggtgaa gccccaggtc cgggcgccgt cgtagacgtc
    29461 gcgggcgacg atcgggccga gcacctggat gggaccctcg atggcgaaca ggaacagaga
    29521 gaccccggcg accatcaccc acagccacgt gtgactcgtg aattcgcgcc agccggcagt
    29581 cagatcacgg agaaagccgc ggctgcccgg tccggcggct gcgatctccc ctccggtcgc
    29641 gccctcttca ccggacaggg cgcgaccagg cgcggcggcc ggggcggccg cctcccggcg
    29701 accgcggaat gccgccggcc gcaggcgcaa gaggaaccag gcgctcgcga gatacgtgag
    29761 tccatcgatg ccgatgcccc agccgggccc gacggcggcg acgagcaggc cggcgaacac
    29821 ggcgccgagc gtgatcgaca tgctcatcac gagtccgctg agcgcgttgg cctgctgcag
    29881 ccgtgcggca ctgaccacct gcggcacgag gccgacggcc gccggcctga agaaggcctc
    29941 agagatgccc atgccgacct ggagcagcgc gagatgccac acctccgcca cccctgcgac
    30001 cagcaacgcc gcggtcgtgc actggagacc gaagcgcagg aagtccgcga ccagcatgac
    30061 cttctgccga gggagccggt cggcccacac gccgccgatg aggaggaaca ccgccagcgg
    30121 tacgagattg gctgcttcga cgatgccgat gtcggtcgcc gagccgccga tctcgagcac
    30181 ggcgaagggg agggccacga tcgtgaacat gacgccgacc acggaggtgg cctgcccgag
    30241 gaacagcttg cggaactcgg gctcacgcag ggcggcggcg ttgtcggcga gcctggtcac
    30301 ctgggaatcc tactcgccgg gccggccggc ggcccgtgcg gagaggcggg ccgtgggtgc
    30361 gccggcgcgc cgccggctac tcgatccgcg gcagccaggc cagccagctc ggcagccacc
    30421 agttccagcg gccgagcagg cgcatggccg ccggcaccat cagcacgcgg atgacggtgg
    30481 cgtccagcgc gacggcgacg gcgaggccga caccgatcgc cttggtcacg acgatcgacg
    30541 tgaacgcgaa cgacaggccc accaccacga tgatgagcgc cgcgctggtg acgatgcggc
    30601 cggtccgcgc gagcccgaag ctcaccgcgc ggacgttgtc gtgggtcgcg tcccactcct
    30661 cgcgcatgcg cgagagcagg aagacctcgt agtccatgga gacgccgaag accgtgcaga
    30721 acagcaccac cggcagttcg gcgtcgatcg cccccgtcgc cgtgaactgg aagagtccct
    30781 ccagattgcc ctcctggaag atccagacca tggccccgta ccccgcgagc agggagcaga
    30841 cgttcatgac gacggccttc acagggagga cgacgctgcg cagcatcagg aagaggacga
    30901 gccctgtcac cccgacgacg aaggcgatga tccagggaaa ccggctgtag atggcgtcca
    30961 cgaagtcgtg gaccgtcagc gaggcgccgg cgacgtacgt ggtcgtgccc ggcgggccgc
    31021 cggcgtcgga gatgtccacg gccagcgcct gggcctcctc ggaggtcggc ggcgcctcgg
    31081 gcacgacgcg gagcagagcc gtgcccggcg ccgtggtcgc cgcggcgagt ttccgggcgc
    31141 ccttgacctg ctcggcgccc agcatgccct ggacgaggcc cggcaggccg gacgcctcag
    31201 tctgcagcgc cccgtcctcg cgcggcgaag cggccgcggc cgcttcgacc ccctgccaga
    31261 acgcggcggc gccggcagct gactccatgc ccggcagcgt cacgatgctg gtgacgcgat
    31321 cgacaccggg gagtgcctcc agttcccgtc caaaggcgtg cagacgcgcg aggttcgcgg
    31381 gcgcgaaggg gtcgtcttcg ccctcccagg tgaccagcac ctcgatgggc gagagcgcgg
    31441 ccgcgtcgaa gcgccgcagc agcaggtcat agcccttccg cgcctcggag tcgcgcggca
    31501 gggccgtcgc ccccgggatg tcggactcga tgcgcaacac cggccaggcg aagacgacca
    31561 ccagcgcgat ggtcccgatc agcacgggca cgggatgccg catgacccag tcgctccagc
    31621 gggtccagaa caccccgccg ccattcttgc gccggatcac gcgcagcgcg tccacccgcg
    31681 gcccgagcag gccgagcagt gcgggcagca acgtcagggc ggcgaggacg ctgacgagca
    31741 cgacgagcgc gccgcccagc cccatcgaac gcagcgacat gtacgggatg atcatgaggc
    31801 cgagcaggcc gacgacgacg gtgaggccgc tgaagaagat ggaacggcct gcggtggcca
    31861 ccgtggtctc caccgcctcg gcgacagcga cgccggcggc gagctcttcc cggaaccggc
    31921 cgaccatgaa gagcgcgtag tcgatgccca cggcgaggcc gagcagcgtc gcgacgttca
    31981 gagcgaagac ggagatatcg aggacctggg cgatgagcca gaagaggccc agggtgacgg
    32041 tcacggccat gccgccgccg atgaccggca gggcggcggc caccagcgtc ccgaagatga
    32101 gcacgagcac gaggaccgcc accgggatcg tgtaggtctc ggccttgcgc aagtcatcgg
    32161 cggaccgctg ctcgagttcc gcgtagacgg ccgggtcgcc ggtcaggagg gtcgtcagct
    32221 ccgtgggctc gagcgccagg cgcagcgccg ggatctgcgc ctgcacctcc tcgctggtgc
    32281 cgtcgaactc cagcacggcg aacgtggccg tgccgtcccc ggagaagaag ctcgcgtcgc
    32341 cggagctcca cgccgtccgg atgccggtca agccgtccag ccctgcctcc tcgacgcccg
    32401 agaggctctc ctccacctgc gcccggaacg ccgggccgcg agcggacagc gtgtcgctgg
    32461 cgaacacgat ggtgaggctc gcgggtccga atccgaggcg ctcctgcatc tggcgctggc
    32521 cctgctggga gggtgacgag gggtcggtga agccgccgcc cttgagctgg gaggccacgc
    32581 tgacgctggc ggtgaggccg gcggcgaacg ccagcgacca cagcacgacg atcaacttgc
    32641 ggcgacggta cacagaccgc ccaagcgcgg cgaacacctc cgacccctcc ccgagatgag
    32701 ccgttcgaac gatatactac gtggaagcaa ggggtccgcc ccgatggcgg gccgatcagc
    32761 atgcctggac ctgtcgaacg cacatccgcc ggtggcgtcg tcgtccgcac ggacgacggg
    32821 gtcgaggtgt gcctcatccg gcctgtggga cgctccgtct ggggactccc caaaggcggc
    32881 gtggaagagg gcgagaccct cgccgagacc gcgctgcgcg aggtcgcgga ggagacgggg
    32941 ctggcgggcg tggtcgagca ggaactgggc agcatcgact actccttcta ctcgcgagag
    33001 cagggcgggc gcatccacaa gaccgtgcac tacttcctgg tgcgcgccac cggcggggac
    33061 acgtcgcagc acgaccacga ggtgagcgac gccaagtggg tgcggctgcg gcaggcgctg
    33121 cggctcatga cctatcccaa cgagcgggag atggtgcggc gggcggccga ggctttgggc
    33181 cgcccggtca ccgactgacg ccggccgcta cgcccagacg atcggctgac gcgcctccct
    33241 cgcctgctcc acgctgcggc cgagcacggt gagcatctcc tgcgcgttca cgtccacgaa
    33301 gtcgtagtgg gcgagcgcga aggtgagctc cggcgtcgca ccgtgacgac cgccgcaggc
    33361 gtcgagctcg cggcgcagac gctccgccgc ccccgcgagc ccgcggcggc tgatgtccgg
    33421 cagcacgagc accaggtcac cgccgtccgt gagaccgacg ctgtcgtagc cacgcaggtt
    33481 ctcgcgcacg caggcgccga ggcggccgac ctcggcgcgc gatgagctgc cggtggcgcg
    33541 ccactctccg tcgtctccct cgacggcgag ctcgaccagg cccagcggca ggtccatccg
    33601 ctggcagcgc tcgacctcga gcgagagccg ctcgccgaac tcggcgggct tcatcgcgcc
    33661 gctgtccgcg tcgagcggcg acgcctccac ggcgtcgcgc cggagccggt cgatcgtgtc
    33721 ggtgaggccc gcggcatagc ctgccgcgac acgggtgagc gccgcgtgct cgagtgcgag
    33781 cagccgccga acggcggcct cggcgccgtc tccgccgcgc cgcagcagcc ccgcacgcac
    33841 gacctcgccg ccacggctgt aggcgtcgag caactctgcc atgggcacct tctgggtgat
    33901 gccggcgcca cggccggcgg attcgagtgc gtcgaggaag gcgctctcgc tgccggcgat
    33961 ggcctcggcc agatgcgaga aggcaggttc ccagagatcc gccgcctcct cgtcgaaggg
    34021 gaggtcggcc gctgagatat cgcgcgtgag atccactccg gctccgatgg ccgcttcagg
    34081 agcgtgactc gctgcgtcgc cttccaggag attgtaccgt cggccagaaa agtacacact
    34141 cgttggcagg ccaacgagga cgaaggtgag gacacatgga cgtcactctg ctcggcacgt
    34201 tgggctggat gccgcgcggc gcgcgggaga ccacctgctt cgcggtccgc gagggaccgg
    34261 cgcttctcgt gttcgacgcc gggaccggcc tccgccgcct cctcgacccc gagcacgccg
    34321 gtctgctcga ggacgcccgc gaggtgcacc tcttcctcag ccactaccac ctcgaccacg
    34381 tctgcggact ggcctacctc tccggggtgt tcgccgggcg ggacgtcgtt ctccatccgc
    34441 ccggccggga gctgacgggc gtcgacccgc tggccgccgt ggcggagctc gtgcgccggc
    34501 cgtacaaccc cgtggacctg gccgacatga aaagggtccg cgtggagccc accgcgcccg
    34561 gcgagaacga cgtcgccgat cacgtggtga gcgtgcgcgc ccagcagcac tcggacacga
    34621 gcgtgtcgtt ccgcctggac gacgccctcg tcatcgcgac cgacacgcag gtcgacccgg
    34681 ccgccgtccc gttcgccgag ggcgccggcc tctggttgca cgaggcctgg tactggtccg
    34741 gcgacccggg cctcgccggc gtgcccgggg agttgcgccc cggatactcg gcgcacagcg
    34801 aggcgacggc cgtcgcgggc ctcgcggcgc aggccgccgt cgcccgcctg atcctcgtgc
    34861 acctcaaccc gctcgcgggc gaggcctcct acctgcccat gagggacgcc gcccgagcgg
    34921 tgttcccacg caccgacgtc gtcgccgacg gcacggtcgc gacgactgcg gcgtccgttt
    34981 gacacgccgg gccgcgcgcc gcgaggattc ctccgtggcc ccgggatgat ccgcttccgc
    35041 gacaagcgct ttctcgccgt ggcgatcgtc gtcgccgcgc tcctcgtgtt cgcgctgccc
    35101 gcgttcctcg ccttccgcta ctcggcgccg gagcagcgtg ggcagttcct ctcgcgtccg
    35161 tggcgcggct ggagcttcgc ctacgcggcc ctcgccgttc ccgggggggc cgagctcaag
    35221 acgtccggca tggcgctgcg caaggccgac tggatctaca agggcacggc cgtcgatccg
    35281 cgtgaggtgc aactcctgtt cgtcagcagc aagcgtccct ataccttcgc gcactccatc
    35341 gacgggcgga ccgtcacgag caccgtcgtc cccagctacc gcttcatctg gcaggtccag
    35401 gggacggtgg gcaccatctc ggacaaggcc gacaccgtcg tcgcgctgct ggactacgac
    35461 tccgggcggc tgctctacga cgtcagggac gacctgacgc cggacgagct cgcgccgcga
    35521 ccggctgacg gcgcgtctcc cagtccggcg ccgtgaggcg cgagaccacc ggcggagggg
    35581 accgttgatc atcgccaccg cgttctacga gacgaactgg ttcaaggcag tcgtctacgt
    35641 cgtcatcgcg atcgtcgtgg cgcgcgtggt cgacttcgtc ctcgcgcgcc gcgacagggc
    35701 catgctcagg ctcctcggca agcagcctga ccacgccgac cgcacccggt acatcatgat
    35761 ccggcggctg gtcttcatcg gcatcctgtt catcggggtg ggcatcgcgc tcatccagat
    35821 cccggcggtc agcacgctcg cacgggccat gctggcctcg gcggcgatca tcgccggcgt
    35881 gatcgggatc gccgcccggg cccccatcgc caacctcgtc agcggcatca tgatcgcctt
    35941 ctcccaaccg gtgcggctga acgattacat cagcgtcgac ggtgagttcg gcacggtgga
    36001 gcagatctcg ctcatctaca cctacatccg cgccggcgac ggccgccgcg tcgtgatccc
    36061 caatgaagcc ttcgcgatga aggcggtgca caactactcc atgggctcac ccggcggcat
    36121 ggtccacgtc gacctctcgc tgccgctcga cgccgacgtg gaacggatcg cccaggcgat
    36181 gctcgaggtc ggcgaatcgc tggctcccgc ccccgtggga aaacaggtca gcgtcgaggt
    36241 cgccgacatc tcgggcggcg gcgtcacgct gcggctgcac gcgtgggcct ccgatccgct
    36301 gcggcggcgc gagatcgcca gtgacctgcg cgccgcgctg ctccgccggc tcaccggcga
    36361 cggcctcctc ggcggggagg aggccggtgg aggcgactga cgtgcagggg ggctccgaag
    36421 acgcggccgg cggcggggag gccgcagccc ccacggtcga ggccgtagcg gccgacgacg
    36481 acggatcagc cgcgggcgcc gagcccgacg cgtccttgcc cagaggcggg cgtcctctca
    36541 cctgggcgct cgccaccgca atcggcagcc gcgtcctgat cttcgccgtc gccttcgccg
    36601 cggccgccct catcggtatc ggcggcctgc ccgtcggctg gcgcttcccg ctccgcgccg
    36661 aggtcttctc cggctggctc ggctcgctct tcaacccctg ggcccactgg gacggcgtct
    36721 ggtacatcaa gatcgccacg agcggctacg ccgaggccga cggcagcacg gcgttcttcc
    36781 cgctcttccc gatgctgctg cgctacgtcg gtgtcctgct cgacggcaac ctgctgatca
    36841 cgggcatcgt catctcgctg ctgtgctacg ccgggtgcgt gtggctcctc taccgccttg
    36901 tgcgacggga cttcgacgag aagatcgccg cccgcgccgt gatctacctc gccatcgggc
    36961 cgctgtcgtt ctttctgcag gccgtctaca cggagtccct gttcctgctc ctctcgctcg
    37021 cctgcttcgt gttcgcccgc gaaggccgct ggcggctcgc gggcgtcatg ggcctgctcg
    37081 cgacgctcac ccgcagcacc ggcgtgcttc tcctcatccc gatggccttc tactactaca
    37141 agcggcgcgg ctgggacttc aggaagaccg actcccacgt ggccaacctg ctcatggtcg
    37201 tcgagggtct cctcgtctgg atgacgtatc tcgccctcgc cttcggcaag ccgctcgcct
    37261 tctcgtccgc gcaggcgcaa tgggagcgcg gcctcggcgc tcccaactac acggtgacac
    37321 gggcggtggt ggccacggtg tgggggttgc gcaaagtcgt ctcggccgag tactaccgct
    37381 ggctctggga ggctccgcgg cccggatccg agtacagcaa tctggccacc aatgtcctca
    37441 acctgctgtt cctcgccgcc gccgcgctgc tgctgtggta cggggcgcgg cggctgcccg
    37501 gtgcctactc ctggtacgcg ctggcggcgc tggcgtaccc gctcttcttc cccagcaagt
    37561 acgtgccgct gatgtcgtat ccgcgcttca cgctcaccgt cttcccgctg tacgtcgccc
    37621 tcgcgctgta caccagcgac cggccgcggg cgcacaaggt ggtggtgtcg ctgggggtcg
    37681 tcctcctcat cgcgttcacg gcgaagttcg ccctcttcag ctgggtggcc tgaaggacgg
    37741 gccagaccct cagggagcgg ccggggaagc cgtctccccc ggcgtcggcg tcgcggcggg
    37801 ggtctggccg ccggggacgg aacccccacc ggcgccgatc cgctcgaaca cgagcacgcc
    37861 gtccttggcg aacaccgagc ggaagttcgg gtcgagcacc agccggccga gggcgagcga
    37921 gtgcatctcc tcgtcctccc tgtcgtagaa gaacgggtgc cgctggtcga cgatgaccca
    37981 atccgccttc gcgaagtacg ggaagatgta cgcgactcgc cgcgccgcga gctgtgcgcc
    38041 ggcgttgttg ttggccgaga ccctggcatc ctcggggatc agcgcgatcg cctcgtcgag
    38101 cacggcgtcg tgcgccgtcc tggcgtagtc ggcgccgccg taggccgctc ccggcaggga
    38161 gaaggggagc ggccccagca cgaagttgcc cgccagggca cagagaagga cgaggagggc
    38221 gagcgtggag cgctggatac gctcgcccct catcagctcc tcactcctgc ggaggccacc
    38281 gcccagccag cgccacagcc gcgagacgcc gagcacggcg gcggcgaaca ggaaggggat
    38341 ctcgagcgcc gtgtagtgga attcgatgcg gcgctggaac accgtggccg acaacgcgtt
    38401 cagcgcgtac tcgggaagcg cgatgagcgc cgtcagcggg ctcaacaccg aggtgaagcc
    38461 caagggccac agcagcgaca gcaggtagcg caggttggac tccgcgaaca cgtcttcagc
    38521 ggtctgtccg gggtgcagca gcgcgttctt cgcgacctct cccgcgccgc tgccgtagtc
    38581 cccatagcgg gagatgaagg cgctctggtc gccgttgtag tgcgggatga cgacccacac
    38641 cgcgacggcg aaccacgcga cggcgagcag ggtcacgacg agcggccacg gcgagcgctt
    38701 gcgcaccgcg aagtaggcgc ccatgaaggc gatcacgagc gggacggtct ctttgcacgc
    38761 cgccgcgagg acgaggaaca cggcggcacg tacccaacgg tcctcctcca tgtacaggaa
    38821 cgcccagagc agcagcggcg tcgccagggc gacggggtgg aactcgttga ggacgaggaa
    38881 gccgagcgcc ggatacagca ggtacgcgcc cgcgaagaac gcccctgccg tcgggtcgcg
    38941 catgacgcgc gtgcccagcc tgaaggccgg ccacgcgccg gtggccgcga tcgcggcctg
    39001 gccgacgagc agcatcaccg ggctcggcca gaccagccac ggcagtgcga acagcgcgag
    39061 gatggggtcc acgtgcgagc cgagccgcga catctgccgc ggctcgaggt ctccggtggt
    39121 gatctcgagg aagtggccgt gcgccgtgtt gtacacggcc tgcaccatgt tgccgaggtc
    39181 ataacgagcg tccatgtacg cccggtactt gagccacgaa agcagggcgt agaccaccgt
    39241 gaagaggatc gcgccgccga tcacgtagag ggcgcggcgg cgcagcagga tcttcatgcg
    39301 ctcatcgtcg agctcctcga tgggagcggg cgcgaagaag ccgccggccg gccccgtcat
    39361 gcgcgtcggg gccttcgccg tccgcctgcg ccggctcacg ccgcgcccct ccggcgcacg
    39421 agcagctcga cgccgacgac gacaccgacg aacaccagtt ccatcgccgt cacccacagg
    39481 cgcagcagca gcgcccaggt gagggcgacg ccgccgggca gctccctgga cagcgacgcg
    39541 gtgagcaccg cttcacgcac gccgatgccg ctcgggatga tgaacgcgac catcccggcc
    39601 acgtaggcga gcgcgtaggc gacgatcacg agcggaagcg cgtccacacc gaggccggtg
    39661 acggcccgcg ccagcagcca cgcgcccgtt cccgcgacga gccagtcgag cgtgaacagg
    39721 atcagcatca cgagcacgga accgaacccg acggtgacct cgagcggcgg tcggtgcagc
    39781 agccgcagcg cccatcccgc cagcggcccg aacacgcgag ggtgcatgag ggccacgagc
    39841 acggggatga cgatcaggct gagcgcggtg acgccggggc ggtactccca gaaggggaac
    39901 aggacggcca cggccagcaa cgccccacac acaccgagcg cctgctcgta gaccatcgcc
    39961 gcggtcaccc gctcgatgac caggccacgg gagtgactca tccaggcgcg accgacgaac
    40021 atgaagacgt tgccgggcac gtagcgcgcc aggatcgact tgccccacac cgacgcggcc
    40081 ggccagaacg ggctcggcgc gccgcagccg cgcaggagca accaccagaa cccggcctgg
    40141 gcgaagtagt agacgaggaa ggcggcgccg gaagccgcga gccagccggc gtcgaacgtc
    40201 cagtcgaact cctggatgct gccccacgag cgggccagat aggcggcgag gaagtacacc
    40261 gtcgccgcca cgatcgccgc ctgggccaga cggacgaaga ggcgccggcg cgaagagggg
    40321 cggcggaccg ccccgtcggg ctcccgcgga gcctcctcgg gacgccggcc ggcggtctcc
    40381 ccggcgctca cgcgcgctcc agggcggcga gcagcgcgcc ggcgaccgcg gtcggcgtga
    40441 acgcgcgccg gtacagcacg agggcggccg caccgaggcg ggcccgctcg cgctcgtcga
    40501 gcagccggcg caaggcggcc gcaagggcgg ccgcgtcgcc ggccggcgtg agcagcgcgg
    40561 tctcgccgtc tcgcagccac tcgcgcgcgc cgtcgctgtc cgcgctgatg accggccggc
    40621 cgcaggcgag cgcgtcgaag accttgttgg gcacgacgcg cccggccttc tcggagccgc
    40681 cgaagacgcc caggcagacg tcggcggcga gcgtgtggcg gcgcagctcg ccggcgggca
    40741 gctgaccgag ccactccacg ttcgtcaggc tgcgcgcggc gatcgccgcg cgcaggtcgg
    40801 ctgagagctg cccttcgccg atgagcacga agcggaacgg ctcggctcgg agcagttccg
    40861 ccgcaccgag cacgacctcg gcgccgtgca gcgggctcca cttgccgtac tgcagcaccg
    40921 tgagcggctc gccggggccg agctcccggg cagcgccgtc cgccctcggc tctggcagcg
    40981 caccgacggg gacgaccgcc acgcggcgcc gcgccagccc gaacagtccc tgatagtagg
    41041 cggcgtgcgc ccaggtgtcg gcgagcacca ggtccgggag gcggaaggcg acctcgtcca
    41101 cggcacggat cgcggcggcc ctggcgccgt ccgcgcgcac gagggcgcgg tcgccggcga
    41161 acgtgtccag cagggacacc agtgggtcga acaccaggcg cgcgtggcga aaggcggcga
    41221 gcacacgccc gaacggcacc aggaagtggc cgggatagcc cacgacgacc gcgtcgacct
    41281 cgaggacgac gcggtgctgc gcgaacaggc gcgcgtgcgc cgcgaggacg ccggcggcca
    41341 ggcgcgccgc gccactcgcg ccggccatct cggccgcgct gaggcgcggc aacgcggcgt
    41401 ggaactcgtg gacctcgacg ccgtgttcgc ggaggcccgc gatgagcacc gcgttgcgcg
    41461 ggtagtccgg ctcgtaggtc ccgaagtacg cgacggcccg cgtcacggct gtgcggcggc
    41521 tccggcggcg ccccgcgacg caacgtctcc gccgccttcg gcgggcggct cgggcgccgc
    41581 ggccgacgtc tccggcccga gcagcgcggc gatctgcgcc gggtccggac cgcgtcgtgc
    41641 gcgggaattg gcgatgagct cgccgatcag cccgaggctg agcagctgca cgccgacgat
    41701 gatgagcagc gccccgagga ggagcagcgg gcgctgcccg atgggattgc cgaacacgag
    41761 cttgtcgatg gtcaggtaga cctcgatcag cacgccgacg aggatgagca gcgttccgaa
    41821 cccgccgaag agatgctggg ggcgatgctg gtaccgcccg aggaacagca ccgtgagcag
    41881 gtcgaggtag ccgcgcatgt aacgctgcca gccgtacttg gaacgacccg cggtgcgccg
    41941 gcggtgactg accttggcct ccgtgacgcg gaatccggcc tgcgcggcga cgaccgggat
    42001 gtagcggtgc agctcaccgt agacggagat cgtgtcgacg acctcgcgcc ggtacgcctt
    42061 gaggccgcag ttgaagtcgt gcagcgggat gcccgaggag cggcgcaccg tccagttgaa
    42121 gaaccgggac ggcagggtct tgttcagcgg gtcctggcgc cgctgcttcc atcccgagac
    42181 caggtcgaag ccttgagaga gcggagccgt gaggttgggg atctccgccg ggtcgtcctg
    42241 gcggtcgccg tccatcgtga cgatggtgtc gccgcgggcg gcggcgaagc cgtgggagag
    42301 cgccgccgcc ttgccgaagt tgcgcctcag cttgacgacg cgcacgtcgt cgcgagcttc
    42361 ggcgagcccc tgcatgacct cgaaggagcc gtcgctggat ccgtcgtcca cgaagacgat
    42421 ctcgtagggg ctgccgtacg cggccagcgc ctcgtcgatc tcggcgacga gcggcacgag
    42481 gctgtccgcc tcgttgtaga cggggatcac gagtgagagc agggggccca tacggctctc
    42541 taggatacat cctcggtgca cgctggcggg gggcagaagc gcttggtagc atggagcgac
    42601 gatgctgatg agcaggaaga cctggctcgt gctcgcagtc gccgctctca tcgcggtgtc
    42661 cttcgccgcg agcgggatcg ccgcgaagaa gtcgtccgag gaccggccaa ggtacgtgac
    42721 gcgcgggctt gactacctgc acgcgcggca gctggacaac ggaggattct cgaccgcggc
    42781 gaacacagcc tgggccgtcc tgggcgcggt ggccagcgga gagcgcatgg gctccagcgc
    42841 gtggaccaag aagggcaaga acccgttcga gtacctgcag agcgtcaacc acgaggcggc
    42901 ggccaccagc gccaccgtgg acaatgcgcc cgtctactac gcccgcatga tcatggccta
    42961 cgtagccgtg gaccggcgag accgggtctt cgtcgccggc accccgcacg tcgacctcct
    43021 cgccaagctg tacacctacc aggacatggc cgacggctcg tccaccaaag gcagcttctc
    43081 gccgtccagc tccacgcgga acttcgacgc cgtgaacacc acttcgtggg ccgccctctc
    43141 catgtacaac ttcggcctgt ccaccgagga gcgcttcctg ctcgccgaga cgtggctggc
    43201 gggcatgcag agcgggtcgg acggcgggtt ccccgcggag gccggcaaga gctcggactg
    43261 ccgcaccacg gcgctcgcca tccaggcgct cagcgtcggg cccggcagcc tgtcgtggga
    43321 cgcggcggcg gcgcggcagt tcctcaagga caaccagaac gccgacggcg ggttcccggt
    43381 gtcgcccggc ggccgcaccg acgccgaagc cacgtcggcc gccatccagg ccatcctcgc
    43441 cctcggcgag catccggacg acgccttctg gatggccggc atcaacaccc cgacgtacgc
    43501 cctcggcgtc ctccagcaga ccaacggcgc gtaccggctg acctcccgcg agcgcctgcg
    43561 cccactcgcc gtgaccagct gggcgctgac ggcgatgcgg cgcaagagct tcgcctcgtt
    43621 ccccaggaac atcggcgtgg cggagacggc gttcaagttc cgccccgtgt tccgcaccgt
    43681 ctcgcccaag aacggcgcca agttcaagtc gcatgtagtc ctgatcaggg cgacctacac
    43741 ggatttctat cccaagggga ccggcatcag gccctcggcc tgccggctct acgtgaacaa
    43801 cgagaacaag agccgcccgg cggacatcga gaagtacggg ctccgcctgc tgctcaagaa
    43861 cgtccccaac ggcgcgcaca cctacaagat cgagttgcgc gaccaggccg gtaacgtcaa
    43921 ggtgatcgag cgccggttca ccgtcgcggt cccgagcccg tctcccacgt acacgccggc
    43981 cccccggcct acctacaatc ccggccctgt ctacccgacg gtacttccca ccagcgtccc
    44041 gacgcccagg ccgtacacca cgcccacccc cacgcccaca ccgacgccgt actccacggt
    44101 gacgccgtac ccgtactaca gcccgtcgcc cgcggcgagc cccgtggtca cagggtcgcc
    44161 gctcccctcg cccagcgcgt cggcgagccc agcgggagca ggtgacggcg aagaaggtag
    44221 tgccgcaggc ttcgtgggtg gtaccctgct cgccatgctt cccatcggcg ccgtcatctc
    44281 ctatctgctc ctgcagcgcc gcgaggagag cctcgctgcg gccagccagg gtacggtgct
    44341 ggacggcgga ggcagcgcct gggatcggtt caagcagacc ctcgcccggt ccaaggacct
    44401 gacgcgacct tcgtcgcggg aatgagccgt cagtgaacga ccttcagcag ctcatctacg
    44461 acatcgccag gatccttctc tggcccgtcc tcatcgccgc cgtcatctgc ctcgtctggg
    44521 tgctcatcga gctcggcgtg ctgctgtacg agctgtggct gcggttccgt taccgcgacc
    44581 ttgacgcgct cgaggtccgc gccctgcgcg cgcgcaaggc gttccgcgac ggcaagccgc
    44641 gcacggccta caggtacctg caggagaaca cctactccct ggtcgtcgtc cgcttcctct
    44701 acgatctcat ccgcaactac cagacggagc gcctggcggc caaaccgctg aagctcctgc
    44761 aggagtacga gttctacacc atcaagcgac tcgagcggac ccggatcctc gtgcgcatcg
    44821 gccccatgct gggcctcatg ggcacgctga taccgctggc gcccgccctg gtgggtctgg
    44881 ccagcggcga tgtccaggag ctctccgaca acctggtcac cgcgttctct gtgaccgtga
    44941 tcggcctgct catcggcggc ctcgcgttcc tggtcagcat cgtgcgcgac cgcctctact
    45001 cccaggacat ctcggacatg gagtatctgc tggagctgct cgaaggctcc gatgtgagac
    45061 tgcgctccgg ccgtcgcctc tccaagaccg gcgtctggga caccgaggcg ccggtcgagt
    45121 acgaggtcgc cgacgaggac gcggagcgca ccgcgatcat cacgctgccg gcgacgccgg
    45181 acgagccgct gctccccgtg gccaccacgc ccggccgcaa cggcggctcc ggacaggccg
    45241 ccgcgcagcg ctcgccggcg gtccccgcag ggccggcgcc ggcggctcgg ccggacgacg
    45301 gcgtcggcct cacgttcgac gaccccagca tggactggac ccgggacgac gacccgttcg
    45361 ccggcctcgg cctcatcgac ccggagggca cggacgatcc caagccccgc gggtccggtg
    45421 ggcccttgcg ccgtcgctga gcgatgccgg cgaacgacga ccactccagg ctgcagaacc
    45481 tcggcaccgt gatgggcgcg cccgagagcg gcttacgcta catgcggcgg cgcagggtcg
    45541 gcacggccga ccgcaacggc gacccgctct acggtctcgc caatctgttc gtggtggcca
    45601 tcgcgctcgc cgtgggcctt ctcgtcgcgg ccctcgcggc ggccggtgtc agtggcttgc
    45661 tcaccagcga gaacatgacg atcgtcacca acccgggcac gcccgagatg caggtgatcg
    45721 tcaaggaggg tgaccagatc accaagctcg acatgcagtc gggggctgag gtggcgggcg
    45781 tgggaacgct catcggctcg ttctacaggc tcgccgacgg caccgtcgtg tacgtcccgg
    45841 cggggacgac ggcgccgacc ggctccacgc cggtacccgg gacgacccct gcgcccgtcg
    45901 tgacgcccac gccggggatc acgccgacgc cgggagcgac gccgacgccg ggagcgacca
    45961 tccctcccac ggcgcccccg acacccggca cgacggcctt ccccacgccg acgccggcgg
    46021 tgacgctcac ccccgcttcg ctctgacgcg cgtcagccga gcgagcgcag gaggtcgacg
    46081 agccgctgca actcggccgc gtctttgaag ccgatctgga tggacccgcc gcgtttgccg
    46141 ctgcggatct tcaccggagc gtcgaacacc ccgtagagtt cgtccgccag ctcggtgaag
    46201 tcccggccgg cggcagggcg aggcgcgggt gcagccatgt ccgtcccctt ctcgcccagc
    46261 ccgcgcacaa gcgcctcgac ctgacgcacc gtgagaccgt cggccaccgc gaggcgcgcg
    46321 acgcggcgtc gctcaccgtg gtcgggcagc gccaggaggg cgcgcccatg cccctccatg
    46381 aggtcgccgc gctcgatcat cgcctgtacc tcatccggca ggtcgagcag gcgcagcagg
    46441 ttcgtgacgg cgacccggct gcgcccgacg agctgggcga tccgctcgtg ggtgagatcg
    46501 aactcgtcca ccagagcggc gtaggccagc gcctcctcga tcgcgttgag atcctcgcgc
    46561 gccacgttct cggcaagcgc gatctccagc gaggagatct cgtccgccgg acgcaccagc
    46621 gcagggatgt agtcgagacc ggcgagcttc gcggcgcgaa gacgccgctc gccggcgatg
    46681 agctcgaagc ggatgccggt gggggcaggc acgcccgcgg ccgcctgccc ctccgggtgc
    46741 agcggccgca ccaccacagg ctggaccacg ccgagcgcct ctatggaggc ggccagctcc
    46801 cgcaattcgt cgtcggcgaa cgtccgccgc ggttgccgcc ggttgacgct gatgtctgcc
    46861 agcggaagct cgcggaggcg gccttcgccc agggtgggga ccgcgccgga gcgccccagc
    46921 gccgcgcggc tctcgccgat caacgccccg aggcccttgc ccagagcctt ctttccgacg
    46981 ccgccggacc gtcccgcgga ctggctctca gtgtcgggat cgcgtgcgtc actcacggga
    47041 gacgacctcc ttggcgagat cgaagtaggc gtcgctgccg gcgcaggtgg catcatagcg
    47101 cgagatcggc aggccgtggc tgggcgcctc gctcagcctg acgttgcgcg ggatgatggt
    47161 ctggaagacg tgctcgccca ggtgctcgcg gacctctccg gcgacctcac gcccgagtcg
    47221 cgtgcgcccg tcgtacatcg tcaccagcac gcccaggatg gtgaggccgg ggttcaggtg
    47281 ttcctgcacc agcccgatcg tctcgagcag cgccgccagc ccctccagcg cgtagtactc
    47341 ggcctgcacg gggaccagga cctcttgcgc cgcgaccagc gagttgaccg tcagcacccc
    47401 cagcgagggc gggcagtcga tgaagacgaa atcatacgtc tcgacgtgac ccgccagcgc
    47461 atactcgaga acgaactcgt tcccgtcggc attggcgagt tcgaccacgg cccccgccag
    47521 gtccgggcac gccggaacga tcgagaggtg ccggagcacg gtcgtcaccg tggcctcggc
    47581 gatgccggcg gcgccaccga gcacaccgta gctggtgagc cgggggtccc cggggcgcac
    47641 gccgaggccg gatgacgcgt tggcctgcgg gtccatgtcg agcaggagca cgtcgaactc
    47701 agcctcagcc aggcaggcgg cgaggttgac ggcggtggtg gtcttgccga cgccgccctt
    47761 ctggttggtc acggcgatga cgcgggtcac cgggctcctt tcgcggggca ccgatactag
    47821 cagagatgac ccgtggtcct ggcgggcgcg gaggcggctc tggtgaaagg gcgcgacccg
    47881 atgggagcgt cgcgagcgcg acgcggtcag acgcccagcg gtcgtttgcc ggccacgccc
    47941 tcgcgtcggg ggagccagtc ggcggtcggc tctctcttcc ggaagacgac gcagacggcc
    48001 tcgtccagtg gcgacgcgga cagcaccacg ggcggctcgg gcgcgaggcc gcaccggcct
    48061 gcggccacgg ccgcggcctc accctcctcc tcgagcgcgc gccgtgtctt gctggccagc
    48121 agcacgccgc cctccgcgag gagaggggcg gcgagctcga cgagcaccgg cagctccgag
    48181 acggcgcgcg cgaggaccac ggtgtaggca tctcgtccgg gcgcgcccgc ggacgcgtgg
    48241 tgctcgctgc gggcgcacac cacgtgcgcg cggccggtga gcccggctgc ggtgacggcg
    48301 gcctcgagga acgcgcactt cttggcgacc gcatcgagca gcgtgacggt cgcgtccggc
    48361 accgccaccg ccagcgggat gccggggatg ccggcgccgg cgccgagatc gagccaggcg
    48421 ccggcctcgc gttgccgcag ctgcggcacc gagagcaggg ccagagagtc gccgatcagc
    48481 gtgtcgacca cggcgacagg gtcggtgagt ccggtcacgt tcgcacgccg ccagccgagc
    48541 agcagctcca cgaaggcgga gagtgcatcg gtctgctcga cctccaggct gtgcgtggct
    48601 gccacgcgct ccagcgcggc ccgcgtcctg aagtctgtgc tcatggttcc cgtcaccgcc
    48661 ctcagccgga cacggcctcc cgcaacgcgc cggcaagagc gtcgacgtcc cccgcgctgg
    48721 tctgccagga gcacatccag cgcacgaccc ccgcatcctc gtcccacacg tagaagtggt
    48781 accgctcgtg cagccgctcg atgacggccg ccggcagcgc cgcgaagacc gcgttggcct
    48841 gcaccggata cgcgagcgtg acacccggtg tggtggcgac cgcatcgccg agccgccggg
    48901 ccatggcgtt ggcgtgcccc gcggtctccc tccagaggtc gccttcgaac aggcggagca
    48961 gctgcgccga gatgtagcgc atcttcgagg ccagctgcgt cccctgcttg cgcgcataca
    49021 ggtagccggc agcgagctcc ggccggaaga agacggcggc ctcggcgccg agcaggccgc
    49081 tcttggtccc ccccagcgtc aacacgtcga cgccacagtc cgtggtcagc tcccgcagcc
    49141 caagtcccag cgcctgcgcc gcgttgacga gccgggcccc gtccatgtgg aggagcagcc
    49201 cggcctcgtg tgccgtctcc gccagcgcgg cgatctcctg cggagcgtag accgtgccca
    49261 gctcggtcga ctgggagatc gagacgaccc gcgcctgcac gtgatgctgg tcgccgaagc
    49321 cgacgagccg ggggcgcagc agctccggcg tcagcttgcc gtccggcgtg ggcacgtcga
    49381 cgagcttggc gccggcgatg cgctccggag cgccgcactc gtccacgttg atgtgcgccg
    49441 tctccgggca gatcaccgcc tcgtaggggc gaagcagtgc ggcgaggacg gtcacgttgc
    49501 cgcccgtgcc gttgaagacg gggaaggcct cgacgtcgcc gagcacctcg cgaaggcgct
    49561 cgcgcaggcg cccggtccag gggtcgtccc cataggcggg cgcccatccc tcgttggcgg
    49621 acgccacggc ctcgagaacg gcgggatgcg ctcccgcgta gttgtcgctg gcgaagagcg
    49681 tgagcggggc actcacgtcg cgaccttctt cttcagcgac tcgtagaaca tgttgagagg
    49741 gaactcgtcg tccagcaccc tgtcgatcca ggcgcgcggc tcggcctcgt cggagtagac
    49801 gcgcgcctcc ttgcgccact gggagccgac gttgggggtc acgtacgggg agatgaggtc
    49861 gtgcgcggcg atccagtacg tcaccttgct ggtctcgatc ccgtgccgat acagctgctc
    49921 gacctgcccg cgcagctcgg cgacggcgga gtcgatccgc tcccagtcga tgagcagccg
    49981 gttgtcggtc caccgcacga cgccgtgcgc gtgcagaaag ccgaagagca gctggccgcc
    50041 cagaccgtcg tagttgcgca cgcggttgcc ggtgatgggg aagcgcagga gacggtcgaa
    50101 gaggatcgcg tacttgacgt agcgcgcgaa ggggaaactc gccgccagct catcggccgt
    50161 gccgtaggtg gcgaggtcga cgcgcagctc ctcgagcgag tacatccagt acggcagccg
    50221 ctggcggatc atgaacgggt cgaagggcag gtcgccgtgg ctgtgccagc gatcgtggat
    50281 catgtcccag aggatgtacg tctcgagcgc caggtccggc gacgacgcca gcgcgcgggc
    50341 gtccggcggc aggtcgatgt cgaggacctc cgcgccccag agcgtggtgc gccgatagcg
    50401 cgccgactcg cggtcacaga agatcgcgcc gaagtggttg gtaggccgct cggccacgct
    50461 caccgtctcg gggaagagca ccgcgcactc gctgtcgtag cccgcggtgt tgtccaccag
    50521 ctgcaccggg acgaacttgg ggttgtcgta gagctcgcgt tcgatctgcg agacgaactc
    50581 cggccaggga gtccgtgtga tcagcgcctc gaagcgcgtg tcgggcgagc cgttgggcgt
    50641 gtacatcgga aagacgacga ggtgctccac gccgtcgcgc cgctcgaggt cggggcggaa
    50701 gagggcgagc gagtccttga agtccggctt gccgcagccg ctctccaccc aggcgtccag
    50761 gtccgcgacg acctgggcca ggtactcctt ctggtgcggg aaccggggcg cgaggtccgc
    50821 gatggcctcg gccatcgcca ccacgagcgc ccgcacctct ccggcatcgt gccggctgag
    50881 gtcgacctcg cccttgggct cctgcagaag acgcagcgcg ccggtcgcct ccttgaggtg
    50941 cagccacgcc ggatcctgcg ccgcctccga gacgatgggc gtcgcgtcgg cgcggaatgc
    51001 ggccagggac acccaataca ggaggttcat ccagagccgg cggttgtcgc gccgcgacag
    51061 gtagtcgtcg ccgaagaggt ccgagtcggc gacgaccacg acgcggccct ccttgtactg
    51121 cgtcgccgcg gcgagcgcag cgccgggcgg gtccgccgtc gcgcgcgtgc gcagtacgac
    51181 ggcgccgggg tcgtctgtgg acagagcccc ggcgcggtag aacccgacct ctccgacgcc
    51241 gtgcaggatg ccggggtcgg cgggtttcgg cgccgactca cccaccaccc acgaggggac
    51301 cccgtccccc gggtcgtact cgaacacgat cgtgttctcg aaccggaccc cgaagggggc
    51361 gaggaggtcg ttgaggttcc cgccgtactt gtcctcctcc tcttctccca gcacgacgag
    51421 gccgccgccg cgggcgacga agtcctgcac ggcggcgatc tccgccggcg agaacacagg
    51481 ggagtctgcg cccaccgtgc gctcccattt gggatcgctc ggatgggcga tgacgaggac
    51541 gtccacgtcc gccagcgcca cttcatccag aggccggccg gccgtggtga cggcgtcgaa
    51601 gtctcgggcc gtgagctccg cggcggctgt cgcgtaggag gcggccgccg gatgggacgg
    51661 ccgcatcctg gccgccgcct ccggccggat cgaccaagcc tctccgtgat gttcgtcgaa
    51721 cagcaccttg gccacggtgc gctggcgcag ggcatcgagc attgggggcc tcctgccctt
    51781 cgaggtcgtg acgcgacttg ggcgcgcgac gtgtcgcgtc ctggtgaagg agttcttcct
    51841 gccccggcgg cggccccaaa cctgccgacg ccgacggcgg cgcggccggc gccgggacgg
    51901 cctgctagag gtcgcggcgc ttggtgagga cgaggaccgt gccgccctcg tccggcttgc
    51961 gcaggtcgaa gtcgtcgacc accgcgcgga tgatggagat gcccataccg ccttcggcca
    52021 gctgatcggt gagcgtgcgg cactccggac agtcgtcgtc gtcctcgtgg aagcccccgc
    52081 cctggtcctt gatgagcacc gtgacgcagt cgctgtgcac ggtgaactcg aggtggacct
    52141 gaccctggtc gtggtcgtac gcatgccgga cggagttgga gcaagcctcc gtgagcgcca
    52201 gcttgaggtc ggcgaccgcg tcgtccgagt atccgccgcg tgagcggaga aggccggaca
    52261 aagcgagccg accgagcgac acgaactcgg ccttcgcggg gatatcgagc ttcacgtagt
    52321 cttccacgac cgctgaatcc tatcgagttg tgggcgttcg ggccaagagc tcagccatta
    52381 cgtctaccgt gacggtcatt tcttgaggga ggtccggacg ttcacgttct gtccgcacat
    52441 cgcacagaat gttccccgga gcgcgacatc ctcaaccgcc gacccggtac ggcggaccag
    52501 gagccgttta cagttcgggc agaccgtgtt ctccccttca tgaccgggca cgttgccgag
    52561 gtacacgaac tccaggccgg cgtcgtgacc ggtcttgacc gccgcctcga gcgtggcgat
    52621 cggcgtcggc gggagatgcg cgaactcgag gtggggcatg aagcgcgtca cgtgccaggg
    52681 cgtctccgcg ccgagccgct ccacgatcca cccggcgatg ccgcgcagcg tcgcggcgtc
    52741 gtcgttgaag gtggggatga cgttggtgac gatctcgacg tggcagccgt gctccgtctt
    52801 ggcacgctcg gccgactcca gcaccggcgt gagatcgggg atcttgcaca gatccttgta
    52861 ctgctgatcc gtgaagccct tcacgtcgac gcggtaggcg tcgatgtgcg gcccgaggat
    52921 cgagagggcg tcatcggtga tgtagccgtt ggtgaccatc accgtgtaca gcccatgctc
    52981 gtgcgccgcc tgggcgccgt ccaggatgta ctcaagccag atcgtgggct cgttgtacgt
    53041 ccaggccaca ccttggcact cgttgttcgc ggccagcacg gagaggttgc ggatgggcag
    53101 gtcgcgcagg tcgcgcgtgg cctccgccgg gtccgcgtgc gcgatctgcc aattctggca
    53161 gtggcggcag gcgaagttgc agcccaggct gccgagcgag agcaccgtgg tgccggggaa
    53221 gaagtggtag agcggcttct tctcgatggg gtcggcggcg acgctgcaca ccttggcgta
    53281 ggtgatggcg tagaggacgc cgtcgcggtt gacccgcgcc cggcacacgc cgct
//
LOCUS       DASUMX010000254         4009 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_954449, whole genome shotgun sequence.
ACCESSION   DASUMX010000254 DASUMX010000000
VERSION     DASUMX010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4009
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_954449"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="VFZ86_12115"
     CDS             <1..622
                     /locus_tag="VFZ86_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276265.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HEX5643063.1"
                     /translation="AEFAALEFGKAMNLQECEVINKQVMHPAEGTLIEMKGRLDVVVD
                     PARLVIPPEPVVMKDEEIKAFVSEKGIKCVAATVGEDEHSVGMREIIDIKHGGLEKWG
                     FSCEYLGTSVPVEKVLDAAIEHAAQVVLISTIITHGDMHRDNMEKLADLAVEKGVRDR
                     LLLISGGTQVTDELARSWGMDAGFGRGAKGIGVASFVVTAMRERGM"
     gene            624..1973
                     /locus_tag="VFZ86_12120"
     CDS             624..1973
                     /locus_tag="VFZ86_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007952733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HEX5643064.1"
                     /translation="MTDPRPVDALVAEIGSTTTVVSAFAGLTDYPQATPCLVAQGVAA
                     TSVADGDVTSGVDAARAQLEAAVGFSVRPELTLATSSAAGGLRMTVHGLTQRMTAMAA
                     REAALGAGAVVKYQTAGRLRAHDLREIDAVRPNLVLLAGGVEGGDCDTVLFNAGELAR
                     LEARPIVVYAGNSVVCSDAQELLERAGFKVKLTNNVYPAVDELDIVPARHVIHDAFEE
                     HITHAPGMERIGEVVNGRILPTPGAVLIAAERLAEDVGDLVVVDVGGATTDVHSITDG
                     SPEIAALTIEPQPHSKRTVEGDLGTYVSARHVVELLAAQERPVRLPPALPETQEEIAA
                     ALTLTHYAAVTGVQRHAGQLAHLYTPTGRQTVARGRDLTACRLVIGTGGALTRLPGGE
                     AVLADTRARSGGGERLLPPPDARVALDRDYIFACCGALLQHFAPEAVTALLRASAGV"
     gene            2112..2648
                     /locus_tag="VFZ86_12125"
     CDS             2112..2648
                     /locus_tag="VFZ86_12125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643065.1"
                     /translation="MTGRVIALVTVAALLILAGCGGAGVPAPSSPPASPESVVTGFVF
                     AYGPHQPKNVLDTPRGTFTKDMILASSITVPFSLSDDELARIFRRIVAIDFWSYPSRY
                     VSRGDGRWFEPPAGMQYETRLTVVTEQGSKTVRWSGGFHADDRRAQDLRDVAALIRRI
                     IESIAEYKRLPPAEGGYL"
     gene            complement(2743..2958)
                     /locus_tag="VFZ86_12130"
     CDS             complement(2743..2958)
                     /locus_tag="VFZ86_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HEX5643066.1"
                     /translation="MAGSVYKVIEIIGTSDTSWEEAAKTAVEEAASHLEDLRVAEVVQ
                     LDMHMVDNKVAAYRAKVKVSFKYHKDA"
     gene            3087..>4009
                     /locus_tag="VFZ86_12135"
     CDS             3087..>4009
                     /locus_tag="VFZ86_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX5643067.1"
                     /translation="MTTPAVHPALAPFTAQVREWFARAFAEPTPAQVQAWPAISAGAH
                     TLVSAPTGSGKTLAAFLWALDRCVAEPPPADPRERTLRLVYISPLKALGYDVERNLKA
                     PLRGIGADVRVGVRTGDTPQRERAAMLRKPPDVLITTPESLYLMLTSQARELFTGTQW
                     VIVDEIHAVAQTKRGAHLAVTLERLAEQAGRDVQRIGLSATQKPLDEVARFLVGPGRE
                     CTIVDAGVRKPLDLRIHVPVESMVEPDAPLELDAIPGGEATRRSIWPAIYPELLRLVR
                     DHRSTLVFVNNRRAAERIALRLNDLARQEAE"
BASE COUNT          576 a         1444 c         1435 g          554 t
ORIGIN      
        1 cgcggagttc gcggcgctcg agttcggcaa ggccatgaac ctgcaggagt gcgaggtcat
       61 caacaagcag gtcatgcacc cggccgaggg aacgctcatc gagatgaagg gccggctgga
      121 cgtggtcgtc gacccggccc gcctcgtcat cccgcccgag cccgtcgtca tgaaggacga
      181 ggagatcaag gccttcgtca gcgagaaggg catcaagtgc gtggccgcca ccgtgggcga
      241 ggacgagcac tcggtgggca tgcgcgagat catcgacatc aagcacggcg gcctcgagaa
      301 gtggggcttc tcgtgcgagt acctgggcac ctcggtgccg gtcgagaagg tgctggacgc
      361 ggccatcgag cacgcggcgc aggtcgtcct catcagcacg atcatcacgc acggcgacat
      421 gcaccgggac aacatggaga agctcgccga cctggccgtg gagaagggcg tgcgcgaccg
      481 gctgttgctc atctccggcg gcacgcaggt caccgacgag ctcgcccgca gctggggcat
      541 ggacgccggc ttcgggcgcg gggccaaggg catcggcgtc gcgtcgttcg tcgtcacggc
      601 gatgcgcgaa cgcgggatgt gacgtgacgg acccgcggcc cgtggacgcg ctcgtcgccg
      661 agatcggctc gacgaccacc gtcgtctcgg cgttcgccgg cctgacggac tacccgcagg
      721 cgacgccctg tctcgtggca cagggcgtcg ccgccacctc ggtcgccgac ggcgacgtca
      781 ccagcggcgt ggacgccgcc cgggcgcagc tggaggcggc cgtcggcttc tcggtcaggc
      841 cggagctgac gctggcgacc agctcggcgg ccggcggcct gcgcatgacc gtccacggcc
      901 tcacgcagcg catgacggcg atggccgccc gggaggcggc tctgggggcc ggcgcggtcg
      961 tcaagtacca gaccgccggc cgcctgcgcg cgcacgacct gcgcgagatc gacgccgtgc
     1021 ggcccaacct cgtcctgctc gccggcgggg tggagggcgg cgactgcgac accgtgctct
     1081 tcaacgccgg ggagctcgcc aggctcgagg cgcggcccat cgtcgtgtac gccggcaaca
     1141 gtgtcgtgtg cagcgacgcc caggaactgc tggagcgcgc cggtttcaag gtcaagctca
     1201 ccaacaacgt ctatccggcc gtggacgagc tcgacatcgt ccctgcccgg cacgtcatcc
     1261 acgacgcctt cgaggagcac atcacgcacg ccccgggcat ggagcgcatc ggcgaggtcg
     1321 tgaacggcag gatcctgccg acgccgggcg ccgtgctgat cgccgccgag cggctcgccg
     1381 aggacgtcgg cgacctggtc gtcgtcgacg tcggcggcgc gacgaccgac gtgcactcga
     1441 tcaccgacgg cagcccggag atcgcggcct tgaccatcga gccgcagccg cacagcaagc
     1501 gcaccgtgga gggcgacttg ggcacctacg tgagcgcgcg gcacgtggtc gagctgctgg
     1561 cggcgcagga gcggccggtg cgcctgccgc cggcgctgcc ggagacgcag gaggagatcg
     1621 cggcggcgct gacgctgacg cactacgccg ccgtcaccgg ggtgcagcgc cacgcggggc
     1681 agctcgcgca cctctacacg ccgaccgggc ggcagacggt cgcgcgaggg cgcgacctca
     1741 ccgcctgccg gctcgtgatc ggcaccggcg gcgcgctcac gcgtctgccc ggcggagagg
     1801 ccgtgctcgc ggacacgcgc gccaggagcg gcggcgggga gcgcctgctg ccgccgccgg
     1861 acgcgcgcgt cgccctcgac cgcgactaca tcttcgcctg ctgcggcgcg ctgctgcagc
     1921 acttcgcgcc ggaggccgtg acggcgctcc tgcgcgccag cgccggcgtg tgacgcgccg
     1981 cggcgcgcac gcagcatcgg cagatccgtc cggcgcaccc cgagggaggc tcgcacgtct
     2041 ccctcgtgtg acggctcgca agcggcgggc cgcggagagc gggaacctca ctcgcgaagg
     2101 caggtgcagt catgaccggc cgggtcatcg ccctcgtcac tgtggcagcg ctgctcatcc
     2161 tggcaggctg tggcggcgcg ggagtcccgg cgccctcctc gccgccggct tcaccggaga
     2221 gcgtcgtcac gggattcgtc ttcgcctacg gcccgcacca gccgaagaac gtgctggata
     2281 cgccgcgcgg cacgttcacc aaggacatga tcctggcctc gtcgatcacg gtccccttca
     2341 gtctcagcga cgacgagctg gcgcgcatct tccggaggat cgtcgcgatc gacttctggt
     2401 cgtatccctc acgctatgtg tcgcgcgggg acggccgatg gttcgaacct ccggcgggca
     2461 tgcagtacga gacccgtctc acggtggtca cagaacaggg gagcaagacg gtccgctgga
     2521 gcggcggctt ccacgcggat gaccgccggg cacaggacct ccgtgacgtg gccgcgctca
     2581 tccggcgcat catcgagtcc atcgccgagt acaagcggct ccctccggcg gagggcggct
     2641 atctgtgagg cggtggtgcg tgccggcggc cggagccgcc ggcacgtcag ggaccgtcag
     2701 gcggagtcat ggggagcgat ggcgccgagg cggtcgcgcc gttcacgcat ccttgtggta
     2761 cttgaacgag accttcacct tcgcacggta ggcggcgacc ttgttgtcga ccatgtgcat
     2821 gtcgagttgc acgacctcgg cgacgcgcag gtcctcgagg tggctcgccg cctcctcgac
     2881 cgcggtcttc gccgcctcct cccacgacgt gtcgctggtg ccgatgatct cgatgacctt
     2941 gtagacgctt cctgccatgg ctcctccctc cggggacgtg acggtgcccc atcatcctct
     3001 cgctgcaggc cccgcgtgac gagcgtttgc ccgtccgtcc cggcggcgtg tgatgcttgc
     3061 cccgcccgcg ggcatccaca gtgcacatga cgacgccggc cgtacatccc gccctcgccc
     3121 ccttcaccgc gcaggtccgt gagtggttcg cgcgggcgtt cgcggagccc acgcccgcgc
     3181 aggtgcaggc ctggccggcc atctccgccg gcgcgcacac gctcgtctcc gcgcccaccg
     3241 ggtccggcaa gacgctcgcc gccttcctgt gggcgctcga ccgctgcgtc gccgagccgc
     3301 cgccggccga cccgcgggag cggacacttc ggctcgtcta catctcgccg ctcaaggccc
     3361 tggggtacga cgtggagcgc aacctgaagg cgccgctgcg cggcatcggg gccgacgtcc
     3421 gggtcggcgt gcgcacgggc gacacgccgc agcgggaacg ggcggcgatg ctgcgcaagc
     3481 cgcccgacgt gctcatcacc acgcccgagt cgctgtacct catgctcacc tcgcaggcgc
     3541 gggagctctt cacgggcacg cagtgggtga tcgtcgacga gatccacgcg gtggcgcaga
     3601 ccaagcgcgg cgcccacctc gcggtgacgc tggagcggct ggccgagcag gcgggccgcg
     3661 acgtgcagcg catcggcctc tcggcgacgc agaagccgct cgacgaggtc gcccggttcc
     3721 tggtggggcc gggcagggag tgcacgatcg tcgacgccgg cgtgcgcaag ccgctcgacc
     3781 tgcggatcca cgtgcctgtg gagtccatgg tagaaccgga tgcgccgctc gagctcgacg
     3841 cgatccccgg cggcgaggcc acccggcgca gcatctggcc ggcgatctac cctgagctgc
     3901 tgcgcctggt ccgcgaccac cgctcgaccc tcgtcttcgt gaacaaccgc cgcgcggcgg
     3961 agcgcatcgc tctgcggctg aacgacctcg cgaggcagga ggccgagga
//
LOCUS       DASUMX010000255         8024 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1817509, whole genome shotgun sequence.
ACCESSION   DASUMX010000255 DASUMX010000000
VERSION     DASUMX010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 8024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8024
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1817509"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..957
                     /locus_tag="VFZ86_12140"
     CDS             <1..957
                     /locus_tag="VFZ86_12140"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5643068.1"
                     /translation="VVEEVIEEQAKPLGLWADTWRRLRRNHLALVGLGIIIVFISIGT
                     VEAVFYQMHWHVRASDEATASDAPGYLAPYDPNMVNYDLSPSGLGSPPSWSHPFGTDY
                     LGRDVLSRTLVATRVAMLVGIIAVAIALTLGLILGPVSGYYGGVVDSIIMRAADIFFA
                     FPYILFVLLIMSVLGQGFQNVFIAIGVLGWATFARVVRGQILSVKSMEFVEAARAQGA
                     GDLRIIFRHVLPNSMAPVYVSVAMAIGGAIATEAALSYLGIGIQPPNSSWGLMISDAV
                     NYLAGGNWWWLLFPSIALVTTVFGFISFGNGLRDATDPKLKE"
     gene            960..1985
                     /locus_tag="VFZ86_12145"
     CDS             960..1985
                     /locus_tag="VFZ86_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5643069.1"
                     /translation="MAERLLTVKDLSTHFFTHAGVVKAVNGVSFTLDRGETIGIVGES
                     GSGKTVAAMSLMRLIPDPPGKVVEGSLMLGDKDLRTLNDADMRAVRGNDIAMIFQDPM
                     TSLNPVMKVGKQIAEAVMLHQDKGKSEALRMAAALLARVGIPDADKRIDQYPFEFSGG
                     MRQRAMIAMAISCNPDVLIADEPTTALDVTIQAQIIDVMREMQAQYESGVILITHDLG
                     VIAEISDKVMVMYGGRTVEYGPVDEIFYNAHHPYTWGLLASLPRLDESEKTRLTPIKG
                     QPPNLITLPAGCPFTPRCPYRREQCRDNWPEHESVGLEHGVHCWIPVEERLAIRGDLP
                     QFKEPAA"
     gene            2035..3081
                     /locus_tag="VFZ86_12150"
     CDS             2035..3081
                     /locus_tag="VFZ86_12150"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013132092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HEX5643070.1"
                     /translation="MSLLEVRDLKKHFPIRTGVFKRTTGYVYAVDGVSFAVEKGETMG
                     LVGESGGGKSTVGRTLLRLTEATGGDVTFEGIDVMKASRGDMNTLRKDMQIVFQDPYA
                     SLNPRMRIRDIVGEGLKIHDVGTEAERKKRVAELMDTVGLNSEHAERYPHEFSGGQRQ
                     RIGIARALALNPKLIVLDEPVSALDVSIRAQVLNLLEDLQDEFGLTYLFVSHDLSVVK
                     HISDRVAVMYLGKIVEISDSAVLYDMPQHPYTEALLSAVPIPDPHTERSRRRIVLEGD
                     VPSPANPPKGCVFHPRCPRAQEICTTAMPRLAITGEAGPRHEVACYFPARFPGGQRTI
                     SDEPYREVIPEPQA"
     gene            3116..4048
                     /locus_tag="VFZ86_12155"
     CDS             3116..4048
                     /locus_tag="VFZ86_12155"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEX5643071.1"
                     /translation="MSLFRTSILAALSVIVALTGAGALAACGDGGDAASPSASPDPVV
                     LRVDGRPIRQSAIDALRAEFRLGGTEDTEARALEEAVRREVVRREAERLEVGADAGEV
                     TARRARLVSQLGGEEALTAALAKVPITDAQLRSGLRDGVLREAVQDAKYGSLVATDAQ
                     ARAYYDENRASFRREASAHLEMIQVRAERIGESALRRLGEGRPFDEVARQFSTDPESK
                     AAGGDMGTVTLASLPAVLRRAVFAARPGEVTGPVEGPGGWYLIRVSELQRAGVAPFAD
                     VRPDILEELTRSRRYEALEAWLDAAREKASVERF"
     gene            4117..4806
                     /locus_tag="VFZ86_12160"
     CDS             4117..4806
                     /locus_tag="VFZ86_12160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643072.1"
                     /translation="MRNALILLAVVALLILAMGALNNGTAFDVDYVAGTVSAVSLFWV
                     SAVIAALVFVVGLAAAWFAKSAVAGSRRKLEAELQSTYERLREAEALVARPAPAPEPA
                     PVAAVVPAEVAEAETVVAAEEATVVAGADETAPAREDVTLIAGEEATVVAGEEATVVA
                     DERGTDVASDAPGEPGEQTAVTMAGATPAGEAGAPDGDAGADGADETRAAESPAEDAA
                     AGEGEAGRTGS"
     gene            4900..7476
                     /gene="recJ"
                     /locus_tag="VFZ86_12165"
     CDS             4900..7476
                     /gene="recJ"
                     /locus_tag="VFZ86_12165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEX5643073.1"
                     /translation="MTDPGSWRIAPAPFADVRRLAEELGVSDVLAQVLVRRGLGDPAA
                     ARAFLHPDFRVHDPYLMTGMSDARRRIDQALRRGEAIAIHGDYDADGITATFLLVSVL
                     ADLGADVRWRLPNRFSEGYGVSSVAVDELAGAGVKLLITVDCGINARAEVAQAQALGM
                     DVIVTDHHELEGDPPACTVVTPKLGAYPCRHLAGVGVAFKLAHALLEEPGDELADLPL
                     ALRPYTDLVAVGTIADVVPLIEENRVLTAIGLGRLRSAPRPGLATLLEVAGGRPGAVD
                     AGAVGFRLGPRLNAAGRLEDASIALELLGAADRDAALPLALRLNELNRERQAIEAAML
                     EAAVAMVPDPPPPALVLSSPDWHEGVVGIVASRVAERFHRPAILLSEGDEEAKGSGRS
                     IPAFDLLGAVERTASHLLTFGGHRAACGLRLRRDAIPAFREAFVAEAASALGPDELRR
                     VRDVDAIVGGDELTLGLADELELLAPHGFGNRQVTLLLHAAEVVAPRLTRDKRHAQYR
                     VRCDGNSCQAIHFNFDDLAGLAEPGRHDVALSLGKNDYNGSVSAQVQVRSLHRLHAPQ
                     TDLCPTGCDLSCRDRARGDHLWRLVLGAGGGARMAADDGAAGEAAATAALHAARAAGR
                     IHDRRGRAPVSRLAALVAGGERVLLLVADVARRRPLLTRDVLAPGLPCGGAYVQAACA
                     QRLTAATVAGVVMTTPDLALADPAFASSFAQVVLLDPPLTITTWAGLAAAAPDAGLHA
                     LWGAAEAAFAGRVRETQLDLDAEMRRVWRALAAGSGRFDDALEQELLGGGAVLAPVAT
                     AAAAVEALREAGLLVVDQDGGYHLQRPQSKVDVTRTEAHRRWRNRYQTPDFLQTCLTT
                     QL"
     gene            7416..>8024
                     /locus_tag="VFZ86_12170"
     CDS             7416..>8024
                     /locus_tag="VFZ86_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX5643074.1"
                     /translation="MAQQVSDTRLPADLPDDAALTQVPDQYGPLLDDLFAAIERYDAD
                     PDRELITRAFVAACILHGRQSRKSGEAYIHHPVGTALNAAELKLDSVTIAAALLHDVV
                     EDTGTPLSWIASEFGDEIAMLVDGVTKLTKMHFTSQEEEQAENYRKMIIAMATDIRVI
                     LIKLCDRLHNMHTLCYLSKQKQIQKAKETLEVYAPLAHRLGIH"
BASE COUNT         1149 a         2971 c         2795 g         1109 t
ORIGIN      
        1 gtcgtcgagg aggtcatcga ggagcaggcc aagccgctcg gcctgtgggc cgacacgtgg
       61 cggcggctcc ggcgcaacca cctcgccctc gtcggcctcg gcatcatcat cgtcttcatc
      121 agcatcggca cggtcgaggc cgtcttctac cagatgcact ggcacgtccg cgccagcgat
      181 gaggccacgg cctccgacgc gccggggtac ctcgccccgt acgacccgaa catggtcaac
      241 tacgaccttt caccctccgg gctcggctca ccaccgtcct ggtcgcatcc gttcggcacc
      301 gactacctcg gccgcgacgt gctctcgcgc acgctggtcg cgacccgcgt cgccatgctc
      361 gtcggcatca tcgccgtcgc catcgcgctc acgctggggc tgatcctcgg gccggtctcg
      421 gggtactacg gcggcgtcgt cgattcgatc atcatgcgcg ccgccgacat cttcttcgcg
      481 ttcccgtaca tcctgttcgt gctgctcatc atgagcgtgc tcggtcaggg gttccagaac
      541 gtcttcatcg ccatcggcgt gctcggctgg gcgaccttcg cgcgtgtggt gcgcggccag
      601 atcctgagcg tcaagtccat ggagttcgtc gaggccgcgc gcgcgcaggg cgccggcgac
      661 ctgcgcatca tcttccgcca cgtgctgccc aacagcatgg ccccggtcta cgtctccgtc
      721 gccatggcca tcggcggcgc catcgccacc gaggccgcgc tgagctacct cggtatcggc
      781 atccagccgc ccaactcgtc gtggggcctc atgatctccg acgcggtcaa ctacctggcc
      841 ggcggcaact ggtggtggct gctgttcccc agcatcgcgc tcgtgaccac cgtgttcggt
      901 ttcatctcgt tcggcaacgg cctgcgcgac gcaaccgacc cgaaactcaa ggaataggca
      961 tggcggaacg ccttctcacg gtcaaggacc tctccacgca tttcttcacg cacgccggcg
     1021 tcgtgaaggc ggtcaacggc gtgagtttca cgctggaccg cggcgagacc atcggcatcg
     1081 tcggcgagtc cggctccggc aagaccgtgg cggccatgtc cctgatgcgc ctcatccccg
     1141 acccgcccgg caaggtggtc gaggggtcgc tcatgctcgg cgacaaggac ctgcgcaccc
     1201 tgaacgacgc ggacatgcgg gcggtgcgcg gcaacgacat cgcgatgatc ttccaggacc
     1261 cgatgaccag cctgaacccc gtcatgaagg tcggcaagca gatcgccgag gcggtcatgc
     1321 tccaccagga caaagggaag agcgaggcgc tgcggatggc ggccgccctg ctggcccgcg
     1381 tgggcatccc cgacgcggac aagcgcatcg accagtaccc gttcgagttc tccggcggca
     1441 tgcgccagcg cgcgatgatc gccatggcga tcagctgcaa tccggacgtc ctgatcgccg
     1501 acgagcccac cacggcgctc gacgtgacca tccaggcgca gatcatcgac gtcatgcgcg
     1561 agatgcaggc gcagtacgaa tccggcgtca tcctcatcac ccacgacctc ggcgtcatcg
     1621 ccgagatctc ggacaaggtc atggtcatgt acgggggacg cacggtcgag tacgggcccg
     1681 tggacgagat cttctacaac gcccaccacc cgtacacgtg gggcctgctg gcgtcgctgc
     1741 cgcggctcga cgagtcggag aagacgcggc tcacgccgat caagggccag ccgccgaacc
     1801 tcatcacgct gcccgccggc tgcccgttca cgccgcgctg cccgtaccgc cgcgagcagt
     1861 gccgcgacaa ctggcccgag cacgagtccg tcggtctgga gcacggcgtg cactgctgga
     1921 tcccggtcga ggaacgcctc gccatccgcg gcgaccttcc ccagttcaag gagccggcgg
     1981 catgagcggt ccacctccgg cggcccgctc gtgtacgcgg gaaggcgagg cgtcatgagc
     2041 ctgctcgagg tccgcgacct caagaagcac ttccccatcc gcacgggcgt gttcaagcgc
     2101 accaccgggt acgtgtacgc ggtcgacgga gtcagcttcg ccgtggagaa gggcgagacg
     2161 atggggctgg tgggggagag cggcggcggc aagtccacgg tggggcgcac gttgctgcgt
     2221 ctcaccgagg cgaccggcgg tgacgtgacc ttcgagggca tcgacgtgat gaaggcgtcg
     2281 cgcggcgaca tgaacacgct gcgcaaggac atgcagatcg tcttccagga cccgtacgcc
     2341 tcgctcaacc cgcggatgcg catccgcgac atcgtcggcg aagggctcaa gatccacgac
     2401 gtcggcacgg aggcggagcg caagaagcgc gtcgcggagc tcatggacac cgtgggcctc
     2461 aactccgagc acgccgagcg ctacccgcac gagttctcgg gtgggcagcg ccagcgcatc
     2521 ggcatcgccc gcgccctggc gctcaacccc aagctcatcg tgctcgacga gccggtgagc
     2581 gcgctcgacg tctccatccg cgcccaggtc ctcaacctcc tcgaggacct gcaggacgag
     2641 ttcgggctca cgtacctctt cgtctcccac gacctctcgg tcgtcaagca catctccgac
     2701 cgcgtcgcgg tcatgtacct gggcaagatc gtcgagatca gcgacagcgc cgtgctctac
     2761 gacatgccgc agcacccgta caccgaggca ctgctctcgg cggtgcccat cccggacccg
     2821 cacacggagc ggtcgcggcg acgcatcgtc ctcgagggtg acgtgcccag ccccgccaac
     2881 ccgcccaagg gctgcgtgtt ccatccgcgg tgcccgcgtg cacaggagat ctgcaccacg
     2941 gccatgccgc ggctggcgat caccggcgag gccgggccga ggcacgaggt cgcctgctac
     3001 ttcccggcgc gcttccccgg cggacagcgg acgatctccg acgagccgta ccgcgaggtc
     3061 atccccgagc cacaggcctg aacgcactcc gaagcgcgcg cgtctccgtg acgacatgag
     3121 cctcttccgc acctccatcc tcgccgcgct gagcgtgatc gtcgcgctga cgggcgccgg
     3181 tgcgctcgcc gcctgcggcg acggcggcga cgcggcttct ccctcggcgt cacccgaccc
     3241 cgtggtgctg cgcgtcgacg ggcggcccat ccgccagagc gccatcgacg ccctgcgcgc
     3301 ggagttccgc ctcggcggca ccgaagacac ggaggcccgg gcgctggagg aggccgtgcg
     3361 ccgcgaggtc gtccgccgcg aggcggagcg tctcgaggtc ggcgccgacg ccggcgaggt
     3421 gacggcgcgg cgcgcgcgac tggtgagcca gctgggcggg gaagaggcac tgaccgccgc
     3481 actcgcgaag gtccccatca ccgacgcgca gttgcgcagc gggctgcgcg acggcgtgct
     3541 gcgtgaggcc gtccaggacg ccaagtacgg gtcgctcgtc gcgaccgacg cccaggcgcg
     3601 tgcctactac gacgagaacc gcgcgtcgtt ccgccgcgag gcgtcggcgc acctggagat
     3661 gatccaggtg cgcgccgagc gcatcggcga gagcgcgttg cgccggctcg gcgagggccg
     3721 gcccttcgac gaggtcgcgc ggcagttctc caccgacccg gagtccaagg ccgccggcgg
     3781 ggacatgggc acggtcactc tggcgtcctt gccggccgtc ctccgcaggg cggtcttcgc
     3841 ggcccgcccc ggcgaggtga ccgggcccgt cgagggtccc ggcggctggt acctcatccg
     3901 ggtgtcggag ctgcagcggg cgggcgtcgc tccgttcgcc gacgtccggc cggacatcct
     3961 cgaggaactc acgcggagcc ggcggtacga ggctctcgag gcctggctcg acgccgcgcg
     4021 cgagaaggcg agcgtcgaga ggttctgagg ccccgccctg tgccggccgc ggcgcgttgc
     4081 tatactcggc ccgacagaga ctaccggagg tcccccgtgc gcaacgcgct catcctgctc
     4141 gccgtcgtcg ccctgctcat cctcgccatg ggcgcgctca acaacgggac ggccttcgac
     4201 gtcgactacg tcgccggcac cgtgagcgcc gtgtcgctgt tctgggtctc ggcggtcatc
     4261 gccgcgctcg tgttcgtcgt ggggctggcg gcggcgtggt tcgccaagtc cgccgtcgcg
     4321 ggctcgcgac gcaagctcga ggcggagctg cagtccacgt acgagcgact gcgcgaggcc
     4381 gaagccctgg tcgccaggcc tgcgccggcg cccgaacccg cgccggtggc ggcggtcgtc
     4441 cccgcggagg tggccgaggc agagaccgtg gtcgcggcgg aggaggccac cgtggtggcc
     4501 ggcgcggacg agacggcgcc ggcgcgcgag gatgtgacgc tgatcgccgg cgaggaggcg
     4561 accgtggtgg cgggcgagga ggcgaccgtg gtggccgacg agcgcgggac cgacgtcgcc
     4621 tccgacgcac cgggagagcc gggcgagcag accgcggtga ccatggccgg ggcgacgccc
     4681 gctggcgaag cgggcgcgcc ggacggagac gccggtgcgg acggggcgga cgagactcgc
     4741 gcagcggaga gtccagccga agacgccgcg gcgggcgagg gcgaggccgg ccggaccggc
     4801 tcctgacggc ttcgacggtc ggccttccgc ggtcgggggg cgccgggtgc ggcacgcatc
     4861 cgtcgtgccg gggcggtaca ctcgccgggt gatgcactcg tgaccgaccc aggctcctgg
     4921 cgcatcgcgc cggccccgtt cgccgacgtc aggcgactcg ccgaggagct cggcgtgagc
     4981 gacgtcctcg cccaggtgct cgttcgccgc gggctcggcg atcccgccgc ggcgcgcgcc
     5041 ttcctccacc cggacttccg cgtccacgac ccgtatctca tgacgggcat gagcgacgcg
     5101 cgccgccgca tcgaccaggc gctgcgccgg ggagaggcca tcgccatcca cggcgactac
     5161 gacgccgacg gcatcaccgc gaccttcctg ctggtgagcg tgctcgccga tctcggcgcg
     5221 gacgtccgct ggcgactgcc caaccggttc agcgagggct acggggtctc gtcggtggcg
     5281 gtcgacgagc tcgccggggc cggggtcaag ctgctcatca ccgtcgactg cggcatcaac
     5341 gcccgggcgg aggtggctca ggcccaggcg ctcggcatgg acgtcatcgt gaccgaccac
     5401 cacgagctcg agggggaccc tcccgcctgc acggtcgtca cgcccaagct gggcgcctat
     5461 ccgtgccgcc acctcgcggg agtcggggtc gcgttcaagc tggcccacgc gctgctcgag
     5521 gagcccgggg acgagctggc agacctgccg ctcgcgctcc gcccgtacac cgacctcgtg
     5581 gccgtcggga ccatcgccga cgtggtgccg ctcatcgagg agaaccgcgt gctcaccgcc
     5641 atcgggctgg ggcggctgcg cagcgcgccg cgccccggtc tggcgaccct gctggaggtg
     5701 gccggcgggc gtccgggcgc ggtcgacgcg ggagccgtgg gtttccgtct gggcccccgg
     5761 ctcaacgccg ccggccggct cgaggacgcc tcgatcgctc tggagctgct gggcgccgcc
     5821 gaccgcgacg ccgcgctgcc gctcgcgctc aggctcaacg agctcaaccg cgagcggcag
     5881 gccatcgagg cggcgatgct ggaggcggcg gtcgccatgg tgccggaccc gccgccgccc
     5941 gcgctcgtgc tctcgtcacc agactggcac gagggcgtcg tgggcatcgt cgcgtcgcgc
     6001 gtcgccgagc gcttccaccg cccggcgatc ctgctcagcg agggcgacga ggaggccaag
     6061 ggctcgggac ggagcatccc ggcgttcgac ctgctcggcg ccgtggagcg cacggcgagc
     6121 cacctgctca cgttcggcgg gcatcgcgcc gcctgcggcc tgcgcctgcg gcgcgacgcc
     6181 atccccgcgt tccgcgaggc cttcgtcgcc gaggccgcgt ccgcgctcgg ccccgacgag
     6241 ctgcggcggg tgcgcgacgt cgacgccatc gtcggcggcg acgagctcac gctcggtctg
     6301 gccgacgagc tcgagctgct cgcgcctcac ggcttcggca accggcaggt gacgctgctg
     6361 ctgcacgctg cggaggtggt ggcgccgcgc ctcacgcggg acaagcggca cgcccagtac
     6421 cgcgtgcgct gcgacggcaa ctcctgccag gccatccact tcaacttcga cgacctcgcc
     6481 gggctcgcgg agccgggacg ccacgacgtc gcgctctcgc tcggcaagaa cgactacaac
     6541 ggctcggtga gcgcccaggt gcaggtgcgc agcctgcacc gtctgcatgc tccgcaaacg
     6601 gacctgtgcc ccaccggatg cgacctcagc tgccgcgacc gggcgcgcgg cgaccacctc
     6661 tggcggctgg tgctcggcgc cgggggcggc gcccgcatgg cggcggacga cggcgccgcc
     6721 ggcgaggcgg ccgccaccgc ggcgctgcac gccgcccgtg ccgccggccg catccacgac
     6781 cggcgcggcc gcgcgcccgt ctcgcggctc gccgcgctgg tcgcgggcgg ggagcgcgtc
     6841 ctcctcctcg tcgccgacgt ggcgcgccgc cgcccgctgc tcacgcggga cgtgctcgcg
     6901 cccgggctgc cctgtggggg cgcctacgtg caggccgcct gcgcgcagcg gctcacggcc
     6961 gccacggtcg ccggcgtggt catgaccacg ccggacctcg ccctggccga tccggcgttc
     7021 gcctcctcgt tcgcgcaggt cgtcctgctc gatccgcccc tcaccatcac cacgtgggcg
     7081 ggtctcgccg ccgcggcgcc ggacgccggc ctgcacgcgc tctggggcgc cgccgaggcc
     7141 gcattcgcgg gccgcgtgcg cgagacccag ctggacctgg acgcggagat gcggcgggtc
     7201 tggagggccc tcgccgccgg ctcgggccgg tttgacgatg cgctcgagca ggaactcctt
     7261 gggggaggag ccgtgctcgc gccggtcgcc accgccgcgg ccgccgtcga ggctctgcgc
     7321 gaggccggcc tgctcgtcgt ggatcaggac ggcggctacc atctccaacg gccgcagagt
     7381 aaggtcgacg tcacccgaac ggaagcgcac agacgatggc gcaacaggta tcagacacca
     7441 gacttcctgc agacctgcct gacgacgcag ctctgacaca ggtccccgac cagtacgggc
     7501 cgctgctgga cgacctgttc gcggccatcg agcgttacga cgccgacccc gaccgcgagc
     7561 tcatcacccg cgccttcgtc gccgcctgca tcctgcacgg ccggcagagc cgcaagagcg
     7621 gtgaggccta catccaccac ccggtgggca cggcgctcaa cgccgcggag ctcaagctcg
     7681 actccgtgac catcgccgcc gctctcctcc acgacgtcgt cgaggacacc ggtacgccgc
     7741 tcagctggat cgcgtccgag ttcggcgacg agatcgcgat gctcgtcgac ggcgtcacca
     7801 agctcaccaa gatgcacttc acctcgcagg aggaggagca ggccgagaac taccgcaaga
     7861 tgatcatcgc gatggccacc gatatccggg tcatcctgat caagctctgc gaccgcctgc
     7921 acaacatgca cacgctctgc tatctgagca agcagaagca gatccagaag gccaaggaga
     7981 cgctcgaggt ctacgcgccg ctggcgcacc gtctcggcat ccac
//
LOCUS       DASUMX010000256         8842 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_954794, whole genome shotgun sequence.
ACCESSION   DASUMX010000256 DASUMX010000000
VERSION     DASUMX010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 8842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8842
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_954794"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..232)
                     /locus_tag="VFZ86_12175"
     CDS             complement(53..232)
                     /locus_tag="VFZ86_12175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643075.1"
                     /translation="MRYGGDLVVLFMLGGGVMFTLLSVAAWVTGRRAGAVFLAIVAGA
                     NLAFALGLLDRASGG"
     gene            374..1300
                     /locus_tag="VFZ86_12180"
     CDS             374..1300
                     /locus_tag="VFZ86_12180"
                     /inference="COORDINATES: protein motif:HMM:NF024875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S double cluster binding domain-containing
                     protein"
                     /protein_id="HEX5643076.1"
                     /translation="MTTLPDWTSVLETLAAAGWTARVVAAERIGELRDRVAGVLASGE
                     LPGPTARHIAGELAFAWPDGVPEARAVVVAATPRPLTRATLTVGGEPREVVVPPHYAG
                     YRTVPDGLAAALGEALASSGHAAARFEPPLKALAACAGLARYGRNNVAYVPGLGSYLM
                     LAACATDAPPPADAAWDEPLSLDRCERCSACLRACPSDAIRADRFLLQTDRCLTMANE
                     DEAPFPDWVDPAWHHCAVGCLRCQQACPENAAVELRIASPETFDERETAAVLAATPGD
                     GLPATTREKLERCGLDYSPTLIARNLLALLGS"
     gene            1418..1873
                     /locus_tag="VFZ86_12185"
     CDS             1418..1873
                     /locus_tag="VFZ86_12185"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5643077.1"
                     /translation="MTHPIVLGYDGSACSAAALDETIELARDTSGAQVVLVYCHEPPP
                     GLSCALDPGCAAAKELRDYERRVAAEVEPMLRHAADRVRAAGIQTEVLLMWDDPARAL
                     EAVAHERGSHVIVVGSHGEGRFAGAIGRHTPFELLHHTDVPVLVVPHRH"
     gene            complement(1884..3395)
                     /gene="glpK"
                     /locus_tag="VFZ86_12190"
     CDS             complement(1884..3395)
                     /gene="glpK"
                     /locus_tag="VFZ86_12190"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011526040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEX5643078.1"
                     /translation="MGEATTGKLVLALDTGTTSGRALVIDASGVVLASAQQEVALGTP
                     HAGWVEQDAEELWLAQLATARAALEKAGVAASAVAGIGIANQRETTILWDRATSAPVA
                     PAIVWQDRRTAGFCEELRLAGAEALVRRKTGLLLDPYFSGTKIRWLLDNVPGVRERAQ
                     AGELAFGTVDSWMAWRLSGGRCHVTDASNASRTLLYDIRAGAWDDELLALFGVPRALL
                     PDVVDSSGVVGESDPALLGAAVPIAGMAGDQQAALFGQACTRRGMIKVTYGTGCFLLL
                     HTGDEPAAAPPGLISTVALQRDGRRTYALEGSVFIGGAAVQWLRDGLGIVGSGAEATS
                     LAQSVSSSDGVVFVPALAGLGAPHWDPYARGGIFGLTRGTTAAHVARATLEGIAFQVA
                     DLYQAEADGAAVAPAEVRADGGAAASDLLLQFQADLLGVPVTRAGQPEATAFGAAWLA
                     GLATGVWADEEEVAALWRAGATFAPAAETGARDLQRRWRAAVDAVRGFARETQ"
     gene            3601..4200
                     /locus_tag="VFZ86_12195"
     CDS             3601..4200
                     /locus_tag="VFZ86_12195"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX5643079.1"
                     /translation="MVRATRKLVEGGAMGWRKILEAEHRLVLEVADAAEAECRHIEET
                     GAVRADLVGDMIGFFRYFNDGLHDPKEEGLLFARCHRRGMTEDDEPLEQMIGEHEWCR
                     GKLDGLERTLERMDPGDRAAALSFSASLREFIQVVRCHIEVEEEVFFDTAQHYLTAND
                     RRELTEEFEAVHWDEVEEGVASYWEDLAHRLYMAEVRSV"
     gene            complement(4264..6102)
                     /gene="gltA"
                     /locus_tag="VFZ86_12200"
     CDS             complement(4264..6102)
                     /gene="gltA"
                     /locus_tag="VFZ86_12200"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HEX5643080.1"
                     /translation="MTGIDHELETLAQQGDLASFCPHCHTALNVYDEKTRRVWVSLDV
                     EVDDVCCDLLVNPRLEEFERVCSPCWTGEAIANDVRCPHCRASLIEAEGLCGKCESRV
                     FTIDVSLHTRVAPLSCCTRADCHWHGVPKRTRAKILAEAPRQKKPEQDPRLRVRNFQE
                     VSYGYDSDLAVAEASRCLQCKKPACVDGCPVGVDIPGFVKLVAASDFAGAARLIREKN
                     ALPAVCGRVCPQDDQCEKVCLLGKKGEPLAIGALERFAADFERQTDQVALPARAPKTG
                     CRIAVVGSGPAGLIMAADMAVKGHSVTIFEALHSPGGVLVYGIPEFRLPKAIVEAETG
                     NLRHLGVRFELNSIVGKLYGLTELFDIGFDAVYVATGAGLPGFMGLPGENLCNVVSAN
                     EYLTRVNLMKAYLFPEYDTPAPLGTRVAVVGGGNVAMDCARTALRMGAREVTIVYRRT
                     RAEMPAREEEIQHAEQEGIRFEYLTSPIAYEGDEQGWVREMRCVRMKLGEPDESGRRR
                     PVLVLKSDFLIDVDMVVVAVGAGPNRALFEDARGLERNERGYIRTLTESGRTSVPKVW
                     AGGDIVTGAATVILAMGAARKAADDMHEFLSDGAAAWTEVHAAASG"
     gene            complement(6112..6942)
                     /locus_tag="VFZ86_12205"
     CDS             complement(6112..6942)
                     /locus_tag="VFZ86_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HEX5643081.1"
                     /translation="MHTIVDRQELTKRDLISLTLEAPQIARKIRPGQFVVLRVNETGE
                     RVPLTVAYRDVANGTVTIIFQVVGKSTAMLASLGVGDAIKDLCGPLGVAEHIENIGTV
                     VGIGGGSGIAVLHHLLTGHREAGNHIIGVVGARQKDLLILEDEMRALCDELVVTTDDG
                     SYGVHGKVTDALASLVERHQHIDKVLAVGPLIMMRAITEMTRIYGLPTEVSLNPIMVD
                     GTGMCGGCRCSVGGETKFACVDGPHFDAFKVDFDELIQRNSMYGRDERISLLTSIKAR
                     "
     gene            complement(7077..8438)
                     /gene="lysA"
                     /locus_tag="VFZ86_12210"
     CDS             complement(7077..8438)
                     /gene="lysA"
                     /locus_tag="VFZ86_12210"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEX5643082.1"
                     /translation="MTRMAGTVTVKGGRLFFDGVDVAGLAGRVDTPFFLYSQARIAAN
                     VARLRRAFEARHAASRVFFAGKACSNLWFLEQVRAAGAGIEVNSGGELWKARRAGFSP
                     RQAVFNGVAKSRLELEEAVAAGVRGTVADSLCELRRLDEVARDLGTPARVLPRVDVNV
                     SGGTHPGLETASGGKAGIDIGEAMEAFRLAASSEHLELAGMHLHIGSQITAVEPYLRA
                     LGVALDLLDGVERELGVTVPALDLGGGFAVAYRERPLQPDGDYFFTGLGVEDYAAAIC
                     AEVARRRPGLELWVEPGRSVAADTAILVTRVESEKTKRLRDRRGRVVAVDRWLLVDAG
                     YNTFFDPVLYGWYYPLVPVSRAGAAADGSFRVAGPLCDGGDVYGGDDGTPYRRLPAAT
                     APGDLLAFHDAGAYTLELMTPYNARPTAAAYAVTEGAEVLRIRAPQSREDLVALDEAK
                     PLS"
     gene            8591..8833
                     /locus_tag="VFZ86_12215"
     CDS             8591..8833
                     /locus_tag="VFZ86_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013679881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEX5643083.1"
                     /translation="MPDEAKPALTLDCIGLYCPEPLFQTRESIDAIAVGEVLEVIADD
                     PAAEEDLKRFAKRAGHEVVSLENDGVELRILLRRLK"
BASE COUNT         1208 a         3297 c         3154 g         1183 t
ORIGIN      
        1 ggacagggcc tgacgcggtc gcctcggagc ggcggcggcc ggaagcgccg gatcagccgc
       61 ccgaggcccg gtcgagcagc ccgagggcga aggcgaggtt ggcgccggcg acgatcgcca
      121 gaaagacggc gccggcgcgg cggccggtga cccaggcggc gacggagagc agcgtgaaca
      181 tcacgccgcc gccgagcatg aacagtacga ccagatcgcc gccgtaccgc acgcgcgcct
      241 cctctctcac cgcggcccgt acggccgccc gggcccagtc tgaagccgcc gcgcggccgc
      301 gacaagccgc gcggttgcgg cgcgcccggc agggccgcac actcggggcc gaccgccggc
      361 cccgaggagg acgatgacga cgctgcccga ctggacgagc gtgctggaga cgctggctgc
      421 cgccggctgg acggcgcgcg tggtcgcggc ggagcgcatc ggggagctca gggaccgggt
      481 ggccggggtc ctcgcctccg gcgagctgcc cggccccacc gcccggcaca tcgccggcga
      541 gctcgcgttc gcctggccgg acggcgtccc cgaggcgcgc gcggtcgtcg tcgccgccac
      601 tccccgcccg ctgacgcgcg ccacgctcac ggtgggcggc gagccccgtg aggtggtggt
      661 gccgccgcac tacgccggct accgcaccgt cccggacggc ctcgccgcgg cgctcggcga
      721 ggcgctcgcc tcctccggcc acgcggcggc gcgcttcgag ccgccgctca aggccctcgc
      781 cgcctgcgcc ggcctggccc gctacgggcg caacaacgtc gcctacgtcc ccggcctcgg
      841 cagctacctg atgctggcgg cgtgcgccac ggacgcgccg ccgccggcgg acgccgcctg
      901 ggacgagccg ctgtcgctgg accggtgcga gcgctgcagc gcctgcctgc gcgcctgccc
      961 cagcgacgcc atccgcgccg accggttcct cctgcagacc gaccgttgcc tcaccatggc
     1021 gaacgaggac gaggccccgt tccccgactg ggtcgacccg gcgtggcatc actgcgccgt
     1081 gggctgcctg cgctgccagc aggcctgccc cgagaacgcc gccgtggagc tccgcatcgc
     1141 ctcgcccgag acgttcgacg agcgcgagac ggcggccgtc ctggcggcta caccgggcga
     1201 cgggctgccg gcgaccaccc gcgagaagct ggagcgctgc ggcctcgact actctccgac
     1261 gctgatcgcc cgcaacctgc tcgccctcct cggctcctga ggcggcgcgc ggcccgcgcg
     1321 cctagcacaa gccggcggcg ggaatcatcg tggtgtggag ccccgcctcc ggcgtgaggc
     1381 cggagggcga atggggcctc ggggaagggg atccgccatg acacatccca tcgtcctcgg
     1441 ctacgacggc tcggcctgct cggccgccgc gctcgacgag accatcgagc tcgcccgcga
     1501 caccagcggc gcccaggtcg tcctcgtcta ctgccacgag ccgccgcccg ggctgagctg
     1561 cgcgctcgac ccggggtgcg ccgccgccaa ggagctgcgc gactacgagc ggcgcgtcgc
     1621 ggccgaggtg gagccgatgc tccgccacgc cgcggaccgc gtccgcgcgg cgggcatcca
     1681 gaccgaggtg ctcctgatgt gggacgaccc cgcgcgcgcg ctcgaggccg tggcgcacga
     1741 gcgcggctcg cacgtcatcg tcgtgggctc gcacggcgag gggcggttcg ccggggcgat
     1801 cggccggcac acgccgttcg agctcctcca ccacaccgac gtgccggtgc tcgtggtgcc
     1861 gcaccggcac tgaccgggct gcgctactgg gtctcgcgcg cgaacccccg gacggcgtcc
     1921 accgccgccc gccagcgccg ctgcaggtct cttgcgccgg tctcggctgc cggcgcgaac
     1981 gtcgcgcccg cgcgccacag cgcggccacc tcctcctcgt cggcccacac gccggtcgcg
     2041 agtccggcga gccaggcggc gccgaacgcc gtcgcctctg gctgcccggc ccgggtgacc
     2101 ggcacaccga gcaggtccgc ctggaactgc aggagcaggt cgctcgccgc ggcccccccg
     2161 tcggcgcgca cctccgccgg cgccaccgcg gcgccgtcgg cctccgcctg atagaggtcc
     2221 gcgacctgga aggcgatgcc ctccagcgtg gctcgcgcga cgtgcgccgc ggtggtcccg
     2281 cgcgtgaggc cgaagatgcc gccgcgcgcg tacgggtccc agtgcggcgc gccgaggccg
     2341 gcgagcgccg gtacgaagac cacgccgtcg ctcgacgaca cgctctgcgc cagagatgtg
     2401 gcctcggcgc cactgccgac gatgccgagg ccgtcgcgca gccactgcac ggcggcgccg
     2461 ccgatgaaca cgctcccctc gagcgcgtac gtgcgtctgc cgtcacgctg gagggccacg
     2521 gtgctgatca ggcccggggg cgcggccgca ggctcgtcgc cggtgtgcag cagcaggaag
     2581 cagcccgtgc cgtaggtgac cttgatcatg ccgcggcgcg tgcaggcctg cccgaagagc
     2641 gccgcctgct ggtcgccggc catcccggcg atgggcacgg cggcgcccag cagcgccggg
     2701 tccgactcgc cgacgacgcc gctgctgtcc acgacgtcgg gcagcagcgc ccgcgggacg
     2761 ccgaagagcg ccaggagctc gtcgtcccag gcgccggcgc ggatgtcgta gagcagcgtc
     2821 cgcgacgcgt tgctcgcgtc ggtcacgtgg cagcggccgc cgctgagccg ccaggccatc
     2881 cagctgtcca cggtgccgaa cgcaagctca ccggcctgcg cgcgctcgcg cacgccgggg
     2941 acgttgtcca ggagccagcg gatcttggtg cccgagaagt acggatcgag gaggagccct
     3001 gtcttgcgcc tgaccagcgc ctcggcgccg gcgaggcgca gctcctcgca gaagccggcg
     3061 gtccgccggt cctgccagac gatcgccggc gcgaccggcg cggaggtcgc ccggtcccac
     3121 aggatggtgg tctcacgctg gttcgcgatg ccgatgccgg cgaccgcgga ggcggccact
     3181 ccggccttct cgagcgcggc ccgcgcggtg gccagctggg ccagccagag ctcctccgcg
     3241 tcctgctcca cccagccggc gtgcggcgtg ccgagcgcga cctcctgctg ggcgctcgcg
     3301 aggacgacgc cggaggcgtc gatgacgagc gcccggccgc tcgtcgtgcc ggtgtcgagc
     3361 gccaggacca gcttgccggt ggtcgcctct cccatgccgc ctccgtccgt cgtctccgcc
     3421 cgcgcgcctg cggccgcggg cgtcgtctct ggcccgacat gatacccgct cccggacgca
     3481 gcgcccgtgc ccggagccgg gtcacgccca cccccgctcg tcgggtgaag accccatatt
     3541 ggagtcccac tacgcacttc atggccccgg cgcgggtaca gtgattgtga aaggagtcgc
     3601 atggtgcgtg cgacccgcaa gctcgtggaa ggaggagcca tgggctggag gaagatcctg
     3661 gaggccgagc accgcctggt gctggaggtc gccgacgcgg ccgaagccga atgtcgtcac
     3721 atcgaggaga cgggtgccgt ccgtgccgat ctggtgggcg acatgatcgg cttcttccgc
     3781 tacttcaacg acggcctcca cgaccccaag gaggagggcc tgctgttcgc ccgctgccat
     3841 cgccgcggga tgacggagga cgacgaaccg ctcgagcaga tgatcggcga gcacgagtgg
     3901 tgccgcggca agctcgacgg gctcgagagg acgctggagc ggatggaccc gggtgaccgg
     3961 gcggccgctc tcagcttctc cgcaagcctg cgcgagttca tccaggtggt gcgttgccac
     4021 atcgaggtcg aggaagaggt cttcttcgac accgcccagc actacctcac cgcgaacgac
     4081 cggcgagagc tcaccgagga gttcgaggcg gtgcactggg acgaggtcga ggagggcgtc
     4141 gcctcctact gggaggacct ggctcaccgg ctctacatgg cggaggtccg atccgtctga
     4201 acgggcgcag gagctcctcc aggcggggtc acggcggctg agcgccccgg ggccgtgacc
     4261 tcgtcatccg gaggccgcgg cgtggacctc ggtccaggcc gcggccccgt cgctcaggaa
     4321 ctcgtgcatg tcgtcggccg ccttgcgcgc cgcgcccatc gcgaggatca ccgtcgccgc
     4381 gcccgtcacg atgtcgccgc cggcccacac cttcggcacg ctggtccggc cggactccgt
     4441 gagcgtcctg atgtagccgc gctcgttgcg ctccaggccg cgggcgtcct cgaagagggc
     4501 ccggttaggg ccggcgccca cggcgacgac caccatgtcg acgtcgatga ggaagtctga
     4561 cttgaggacg aggaccggcc ggcggcgccc cgactcgtcc ggctcgccca gcttcatgcg
     4621 cacgcagcgc atctcgcgca cccatccctg ctcgtcgccc tcataggcga tggggctggt
     4681 gaggtactcg aagcggatcc cctcctgctc ggcgtgctgg atctcctcct cccgcgccgg
     4741 catctcggcc cgggtgcgcc ggtagacgat ggtgacctcg cgcgcgccca tgcgcagcgc
     4801 cgtgcgcgcg cagtccatgg ccacgttgcc gccgccgacg accgcgaccc tcgtgcccag
     4861 cggcgccggc gtgtcgtact ccgggaacag gtacgccttc atgaggttga cgcgggtgag
     4921 gtactcgttg gcggagacca cgttgcagag gttctctccg ggcaggccca tgaacccggg
     4981 gaggcccgcc cccgtggcca cgtacacggc gtcgaacccg atatcgaaca gctccgtgag
     5041 cccgtagagc ttgccgacga tgctgttgag ctcgaacctg accccgaggt ggcgcaggtt
     5101 gccggtctcc gcctccacga tcgccttggg caggcggaac tcggggatgc cgtagacgag
     5161 caccccgccg ggactgtgca gcgcctcgaa gatggtgacg ctgtggccct tgacggccat
     5221 gtcggcggcc atgatgagac ccgccgggcc gctgccgacc acggcgatgc ggcagcccgt
     5281 cttcggcgcc cgcgccggca gcgcgacctg gtcggtctgg cgctcgaagt cggcggcgaa
     5341 ccgctcgagc gcgccgatgg cgagcggctc gcccttcttg ccaagcaggc acaccttctc
     5401 gcactggtcg tcttgcgggc agacccgccc gcacacggcc ggcagcgcgt tcttctcgcg
     5461 gatgaggcgg gccgcgccgg cgaagtcgct cgcggccacc agcttcacga acccggggat
     5521 gtcgacgccg accggacagc cgtcgacgca ggccggcttc ttgcactgca ggcagcggct
     5581 cgcctccgcc acggccaggt cgctgtcgta gccgtagctg acctcctgga agttgcgcac
     5641 gcgcagcctg gggtcctgct ccggcttctt ctgccgcggc gcctcggcga ggatcttggc
     5701 gcgcgtcctc ttgggcaccc cgtgccagtg gcagtccgcg cgcgtgcagc acgagagcgg
     5761 cgcgacgcgc gtgtgcaacg acacatcgat ggtgaagacg cgcgactcgc acttgccgca
     5821 caacccctcg gcctcgatca gcgaggcgcg gcagtgcggg cagcggacgt cgttcgcgat
     5881 cgcctcgccg gtccagcagg gcgagcagac gcgctcgaac tcctcgagcc tcgggttcac
     5941 gagcaggtcg cagcagacgt cgtccacctc cacgtcgagg ctgacccaga cgcgcctggt
     6001 cttttcgtcg tagacgttca gcgccgtgtg gcagtgcggg cagaagctcg cgagatcgcc
     6061 ctgctgggcg agcgtctcga gctcgtggtc gatgccggtc atcacggcct cctaccgcgc
     6121 cttgatggac gtgagcagcg agatgcgctc gtcgcgcccg tacatgctgt tgcgctggat
     6181 cagctcatcg aagtcgacct tgaacgcgtc gaagtgaggg ccgtccacac aggcgaactt
     6241 cgtctccccg ccgacgctgc agcggcagcc gccacacatg ccggtcccgt cgaccatgat
     6301 cgggttgagg ctgacctcgg tgggcaggcc gtagatgcgc gtcatctcag tgatggcgcg
     6361 catcatgatg agcggcccga cggcgaggac cttgtcgatg tgctggtgcc gctcgaccag
     6421 gctcgcgagc gcgtcggtca ccttgccgtg cacgccgtag ctgccgtcgt cggtggtcac
     6481 gaccagctcg tcgcagaggg cgcgcatctc gtcctcgagg atcaggagat ccttctggcg
     6541 cgccccgacc accccgatga tgtggttgcc ggcctcgcgg tgcccggtga gcaggtggtg
     6601 cagcaccgcg atgccggatc cgccgccgat gcccaccacg gtgccgatgt tctcgatgtg
     6661 ctccgccacg ccgagggggc cgcacaggtc cttgatggcg tcgccgacgc cgagcgacgc
     6721 gagcatcgcg gtgctcttgc cgacgacctg gaagatgatg gtgacggtgc cgttggccac
     6781 gtcgcggtaa gcgacggtga gcggcaccct ctcccccgtc tcgttcacgc gcaggacgac
     6841 gaactgcccg gggcggatct tgcgcgcgat ctggggcgcc tcgagcgtga ggctgatgag
     6901 gtcgcgcttg gtgagctcct gccggtcgac gatcgtgtgc atggccagtc ccccctgacc
     6961 cgatgactcg atgacgtcgc gcggccgctg cggtcgcgtg gctcctggtc tcactatacc
     7021 catccgccac ggttgcgcat ggggtgaaga cccgggcgga agcggccggg acgcgctcag
     7081 gacagcggct tcgcctcgtc gagcgccacg aggtcctcgc gggactgcgg ggcgcggatg
     7141 cgcagcacct ccgcgccctc ggtcaccgcg taggcggcgg ccgtcggcct ggcgttgtac
     7201 ggggtcatga gctccagcgt gtaggcgccg gcgtcgtgga aggcgagcag atcgcccggc
     7261 gcggtcgccg ccggcaggcg ccggtagggc gtgccgtcgt ccccgccgta cacatcgccg
     7321 ccgtcgcaca gaggcccggc gacgcggaag ctcccgtcgg ccgccgcgcc ggcgcggctc
     7381 accgggacga gcgggtagta ccagccgtac agcacggggt cgaagaaggt gttgtagccg
     7441 gcgtcgacca gcagccagcg gtccaccgcc acgacccgcc cgcgccggtc cctcaggcgc
     7501 ttggtcttct cgctctccac gcgcgtgacc aggatcgccg tgtcggcggc gacggagcgg
     7561 ccaggttcga cccagagctc gagccccggg cgccgccgcg cgacctccgc gcagatcgcg
     7621 gcggcgtagt cctccacgcc gagaccggtg aagaagtagt cgccgtccgg ctgcaggggg
     7681 cgctcgcggt aggcgacggc gaagccgccg cccaggtcga gcgccgggac ggtcacgccc
     7741 agctcgcgct cgacgccgtc gagcaggtcg agcgcgacgc cgagcgcccg caggtacggc
     7801 tctaccgcgg tgatctgcga gccgatgtgg aggtgcatcc ccgcgagctc gaggtgctcg
     7861 gacgacgcgg ccaggcggaa cgcctccatg gcctcgccga tgtcgatgcc ggccttgcca
     7921 ccgctcgccg tctcgaggcc ggggtgcgtg cctcccgaga cgttgacgtc gacgcgcggc
     7981 aggacgcgcg ccggcgtgcc gaggtcgcgc gcgacctcgt ccagccggcg cagctcgcag
     8041 agcgagtcgg cgacggtgcc gcggacgccg gccgcgaccg cctcctcaag ctcgaggcgg
     8101 ctcttggcga cgccgttgaa cacggcctgg cgcggcgaga acccggcgcg ccgcgccttc
     8161 cagagctcgc cgcccgagtt cacctcgatg cccgcgccgg cggcgcggac ctgctcgagg
     8221 aaccagaggt tggagcaggc cttgccggcg aagaacacgc gactcgccgc gtgccgcgcc
     8281 tcgaacgccc gccgcaggcg ggcgacgttc gcggcgatcc gcgcctgcga gtagaggaag
     8341 aacggggtgt cgacccggcc ggccaggccg gcgacgtcga ccccgtcgaa gaacagccgg
     8401 ccgcccttca ccgtcaccgt ccccgccatc cgtgtcatcc gccgcctccc cgccgtccct
     8461 gcgccgccag cctaccagag cccggccaaa cccctcccgg cgcgcagtga ataccaaccg
     8521 tgttggtcgg gtatagaccg cgctggaggg agtgcagtca cgcctccgca cctgcgaagg
     8581 gagaaaccac atgccagacg aagcgaagcc ggccctcacg ctcgactgca tcgggctgta
     8641 ctgtcccgag ccgctgttcc agacgcgcga gagcatcgac gcgatcgcgg tcggcgaggt
     8701 cctggaggtc atcgccgacg atcccgccgc cgaggaggac ctcaagcgct tcgccaagcg
     8761 cgccggtcac gaggtcgtct cgctcgagaa cgacggcgtc gagctccgca tcctcctccg
     8821 caggctcaag tagaagacga aa
//
LOCUS       DASUMX010000257         2673 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_523048, whole genome shotgun sequence.
ACCESSION   DASUMX010000257 DASUMX010000000
VERSION     DASUMX010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_523048"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1754
                     /gene="ptsP"
                     /locus_tag="VFZ86_12220"
     CDS             <1..1754
                     /gene="ptsP"
                     /locus_tag="VFZ86_12220"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEX5643084.1"
                     /translation="APGIVRAEPPAAGAVLTGVPVSPGAAAGPARLLRRRAVVAPGRA
                     AADPPAEWSALERALEATAADVRRAGAAVGARAAAADAAIFDAHLLFLEDDALLVPAR
                     AGVFDRHETAARAWTDAVSAAAEVWHGLEDPYQRARATDLRDVGEQVLAHLAGPGETT
                     AVVEAGDVVVAPDLAPADVAALASSAVAGIALAHGGPASHAAILARALGLPAVAGAGE
                     ALLAVPDGTALLVDGDAGAVTVAPPSEAVAEVARRRALQARATAEARAAATAPAVTRD
                     GVTVRVEANIAGPQDVPAALAGGADGVGLLRTEYLFLDAATMPGEDEQAAAYSAVAEA
                     LGGRPLTVRTLDAGADKPLAFLAPAAEANPFLGVRGLRLSLAQPGLLRTQLRAVLRAA
                     AGHDLRVMFPMVSTVDELRRAREILDEARASLRDEGVATPAELEVGVMLEVPSAALVA
                     EKLAPLVAFFSVGSNDLTQYTLAAERGNAAVAALSDPLHPAVLRLIRLTAEAAAAAGR
                     RMAVCGEVAGDRLAVPLLIGLGVTELSMNAVAIPAAKQAVRATDAARAAGLAEQALDA
                     ESAAAVRRLLAAAAPSA"
     gene            1809..2414
                     /locus_tag="VFZ86_12225"
     CDS             1809..2414
                     /locus_tag="VFZ86_12225"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003153301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HEX5643085.1"
                     /translation="MTSGSDDTARILAMVGSLRRGSYNRLLYEAAVELAPAGMTFIEA
                     DLRSLPLYDDDVRLAQDYPEPARRLRADIAAADGVLIVSPEYNHTVPGVLQNAIDWAS
                     RPPDQPFKGKPVAIMGASTGRLGTVRMQLTLRTTLDSLEAHALLKPEVMVSSAREAFD
                     ETGLADPTARQLVAAQMAAFGAWIGRLSPAPQPAPDDAGGR"
     gene            complement(2441..>2673)
                     /locus_tag="VFZ86_12230"
     CDS             complement(2441..>2673)
                     /locus_tag="VFZ86_12230"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEX5643086.1"
                     /translation="VAGLDPLEAALAGVRELDRLTDAVGIPTLAQLGFSEDEIPLLAR
                     IAFDDPQTVGNPRELGVADYESIYRSAFARGA"
BASE COUNT          298 a         1006 c         1055 g          314 t
ORIGIN      
        1 aggcgccggg aatcgttcgc gccgagccgc cggcggccgg cgccgtgctc accggcgtcc
       61 cggtgtcgcc gggcgcggcc gccgggcccg cgcgcctgct gcgccggcgc gccgtagtgg
      121 cgccgggccg ggccgccgcg gacccgccgg cggaatggag cgccctggag cgggcgctcg
      181 aggccacggc ggcggacgtg cggcgggcgg gtgcggcggt gggcgcccgc gccgcggcgg
      241 cggacgcggc gatcttcgac gcccacctgc tgttcctgga ggacgacgcg ctgctggtcc
      301 ccgcgcgcgc gggcgtcttc gaccgccacg agacggcggc gcgcgcgtgg accgacgccg
      361 tctccgccgc ggccgaggtg tggcacgggc tggaggatcc gtaccagcgt gcccgggcga
      421 cggacctgcg cgacgtggga gagcaggtgc tcgcccacct cgcagggccc ggcgagacca
      481 ctgccgtggt ggaggccggc gacgtcgtcg tggcgcccga cctggcgccg gcggacgtcg
      541 ccgcgctggc gtcctcggca gtcgcgggca tcgccctcgc ccacggcgga ccggcgtcgc
      601 acgcggcgat cctcgcccgc gccctcggcc ttccggccgt ggccggcgcg ggcgaggcgc
      661 tcctggccgt ccccgacggc acagcgctcc tcgtggacgg cgacgccggt gcggtgaccg
      721 tggcgccgcc gtccgaggcg gtcgcggagg tcgcacggcg acgagccctg caggcgcggg
      781 cgaccgccga ggcgcgcgcc gcggccacgg cgcccgccgt cacgcgcgac ggcgtcaccg
      841 tgcgcgtgga ggcgaacatc gcggggccgc aggacgtgcc ggctgcgctg gccggcggcg
      901 ccgacggcgt gggcctgctg cgcaccgagt acctgttcct ggatgccgcg accatgcccg
      961 gcgaggacga gcaggccgcc gcgtactcgg ccgtggccga ggcgctcggc ggccgtcccc
     1021 tcacggtgcg caccctggac gccggggccg acaagccgct cgccttcctg gcaccggcgg
     1081 cggaggccaa ccccttcctc ggcgtgcgcg gcctgcggct gagcctggcg cagcccgggc
     1141 tgctgcgcac gcagttgcgc gccgtgctgc gcgccgctgc gggacacgac ctgcgcgtga
     1201 tgttcccgat ggtctcgacc gtcgacgagc tgcggcgtgc ccgggagatc ctggacgagg
     1261 cccgggcctc cctgcgcgac gaaggcgtcg cgacgccggc ggagctcgag gtcggggtga
     1321 tgctcgaggt accttccgcc gcgctcgtcg cggagaagct ggcccccctg gtcgcgttct
     1381 tctccgtggg ctccaacgac ctgacgcagt acacgctcgc ggcggagcgc ggcaacgccg
     1441 cggtcgcggc gctctcggac ccgctgcacc cggcggtgct gcggctcatc cgcctcacgg
     1501 cggaggcggc cgcggccgca gggcggcgca tggcggtgtg cggcgaggtg gccggcgacc
     1561 ggctcgccgt ccccctcctc atagggctcg gcgtgacgga gctctccatg aacgccgtgg
     1621 ccatccccgc tgcgaagcag gcggtgcgcg cgacggacgc ggcgcgggcg gcaggtctcg
     1681 ccgagcaggc gctggacgcg gagtcggcag ccgccgtgcg gcggctgctg gccgccgcgg
     1741 ccccttccgc gtagcctccc gagggcgtac cctccacccc atcaccgagc gaccggggag
     1801 tgcccgacat gacgagcgga tccgacgaca ccgcgcgcat cctggcgatg gtcggcagcc
     1861 tgcgccgcgg ctcgtacaac cggctgctgt acgaggcggc ggtcgagctg gcgccggcgg
     1921 ggatgacgtt catcgaggcg gacctccgct ccctgccgct ctacgacgac gacgtgcgcc
     1981 tggcgcagga ctatcccgag ccggcgcggc gcctgcgggc ggacatcgcc gcggcggacg
     2041 gcgtcctcat cgtctcgccg gagtacaacc acacggtccc gggcgtgctg cagaacgcca
     2101 tcgactgggc gtcgcgccca ccggaccagc ccttcaaggg caagccggtg gcgatcatgg
     2161 gcgcgtcgac ggggcggctg gggacggtca ggatgcagct caccctgcgg accacgctcg
     2221 actccctgga ggcgcacgcg ctgctcaagc ccgaggtgat ggtcagctcc gcgcgcgagg
     2281 cgttcgacga gaccgggctg gcggacccga cggcgcgtca gctggtggcg gcgcagatgg
     2341 cggcgttcgg ggcctggata gggcgcctgt ctccggcgcc acagccggcg ccggacgacg
     2401 ccggcgggcg ctgagccgtc ctgtcaggcg cgtccgaccg tcaggcgccg cgcgcgaagg
     2461 cggagcggta gatgctctcg tagtccgcga cgcccagctc acgcgggttc cccacggtct
     2521 gcggatcgtc gaaggcgatc cgcgccagca gcgggatctc gtcctcggag aagccgagct
     2581 gcgcgagcgt cgggataccg accgcgtcgg tcagccggtc gagctcgcgg acgccggcca
     2641 gcgccgcctc cagcgggtcg aggccggcga cgt
//
LOCUS       DASUMX010000258         3539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_92917, whole genome shotgun sequence.
ACCESSION   DASUMX010000258 DASUMX010000000
VERSION     DASUMX010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3539
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92917"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            224..634
                     /locus_tag="VFZ86_12235"
     CDS             224..634
                     /locus_tag="VFZ86_12235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643087.1"
                     /translation="MIWAILAFLGVPLWIIALALVALYLRNRALRNRPGDIAVRILRP
                     GKRRWTRAHALWVSDVLAWRGSPAAWREDLVQVRSVYLHAPDAEQQKKLHGLGDDPVL
                     ASLSLTGGSSLEVAARHEHRAALLGPFGEATEGA"
     gene            complement(715..1020)
                     /locus_tag="VFZ86_12240"
     CDS             complement(715..1020)
                     /locus_tag="VFZ86_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HEX5643088.1"
                     /translation="MSEKRLPPVHPGEVLLEEFLKPLGLSQNRLGLSLGVHPRRINEI
                     VLRKRRVTAETALRLGRYFGTSPQFWLGLQSDYDLDVAADELGERLEREVQVRAKAG"
     gene            complement(1035..1316)
                     /locus_tag="VFZ86_12245"
     CDS             complement(1035..1316)
                     /locus_tag="VFZ86_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002740810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX5643089.1"
                     /translation="MIKSFRDSETERIFDRRFSRRLPNDIQAVALRKLRMLNNAVDLV
                     DLRSPPANRLEKLSGDRAGQHSIRINDQWRICFRWRGGDAHEVEICDYH"
     gene            1647..2120
                     /locus_tag="VFZ86_12250"
     CDS             1647..2120
                     /locus_tag="VFZ86_12250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643090.1"
                     /translation="MKKWKLTWIVMAVAVAVLALAGVALAATDAPSPAATPGAGVACG
                     VTDDPEALAELQALRTDFWDARQAWFDKYGAERTSDEAQAAFQKLRDDRIAKTQAVFD
                     KYGIDATAGSRAGGGYGRGAMMGGGYGNGGMMGGGYGNGGMMGGGCGGCALSGTD"
     gene            complement(2203..2820)
                     /locus_tag="VFZ86_12255"
     CDS             complement(2203..2820)
                     /locus_tag="VFZ86_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012822995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEX5643091.1"
                     /translation="MGADENEYDEAAELERRTLLVLLGINGALFVVEAVAGWIGQATG
                     LVADSLDMLADAFVYAIALSAVGRSRRLQSNAAAVSGVLQIALGLLVVADVARRLLAG
                     SEPVSLLIVGIGLVALAANLTCLALISKHREGGIHMRASWIFSANDVIANIGVIVAGV
                     LVWLLGSRIPDLVIGAAIAVVVIRGGVKILLEVRTEKAEAGSWTH"
     gene            complement(2999..3451)
                     /locus_tag="VFZ86_12260"
     CDS             complement(2999..3451)
                     /locus_tag="VFZ86_12260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643092.1"
                     /translation="MARARGSDLVIVVVALAVAALATVGIFALGSRGSADLSPVSRGE
                     AIFQSGVDADGDLIPRSSSGGGMMGEGGMMGGGCATCHGTDGRGRTTAAFTAPNITYD
                     NLTDPRGMLEPDGGRGPTFSDATLRTAVIEGLDPEGSRLEARRCRSGS"
BASE COUNT          534 a         1224 c         1251 g          530 t
ORIGIN      
        1 ggtgggggtc gccgcgacct tggcgggcgt ggcgctgatc gcggcaggct gacgcccgcg
       61 gcggcgccat cgcgtgattg cgcctggggt taccggtcgg ttatgctcgc gcgacccgcg
      121 cgccgccctg gatgcatgcg gtcctatcgg tgaaggagtc gcacatggtc gcaggagtcc
      181 ccggggcgtt ttcgagactc ggccccgcgc tgggggcagt ctcgtgatct gggccatcct
      241 cgcctttctc ggggtcccgc tctggatcat cgcgctcgcg cttgttgcgc tctacctccg
      301 caaccgcgct ctgcgcaatc gtccgggcga catcgcggtg cgcatcctgc ggccgggcaa
      361 gcggcgctgg acgcgggccc acgctctgtg ggtctcggac gtgctcgcct ggcgaggaag
      421 ccctgcggcc tggcgcgagg acctggttca ggtgcgcagc gtctacctcc acgctccgga
      481 cgcggagcag cagaagaagc tccacggtct cggcgacgac cccgtgctcg caagcctctc
      541 gctcaccggg ggctcgtcgc tcgaggtggc cgcgcgccac gagcaccggg ccgcgttgct
      601 cggccccttc ggagaggcga ccgagggcgc ctgagcggtc ccggtcgcgc gtccgggcga
      661 gcgtcgcact gcgaacccga gctcacgcgt acttggactc gggtgcctcg agcactagcc
      721 tgccttagcg cggacctgga cctctcgctc caaccgctct ccaagctcgt cggcagcgac
      781 atcgaggtcg tagtcggact gcagcccgag ccagaactgc ggcgaggtgc cgaagtagcg
      841 ccccaggcgc agcgccgtct cggcggtgac gcggcgcttg cgcaggacga tctcgttgat
      901 gcgccgcggg tgcacgccga ggctcaggcc cagccggttc tggctgaggc ccagcggctt
      961 caggaactcc tcgagcagca cctctcccgg atggaccgga gggagcctct tctcactcat
     1021 gcgcgctcac ttcctcagtg gtagtcgcag atctcgacct cgtgggcgtc gccgccgcgc
     1081 cagcgaaagc agatgcgcca ctgatcgttg atacggatac tgtgctggcc agcccggtcg
     1141 ccggagagct tctcaagccg gttggcgggt ggactgcgca gatcgacaag atcgacggcg
     1201 ttgttcagca tccgcagctt gcgcagcgcc accgcctgga tgtcattcgg cagcctgcgg
     1261 gagaacctgc ggtcgaagat cctctccgtc tcgctgtcgc ggaacgactt gatcatgggg
     1321 gctatggtat aacgcgtcgc gttatacgtc aaaggaggca gcgcctcgag gagaaccacg
     1381 gaggggtagt cgatgcacgt caccgccgtt gtgggatcgt tgcgcgacgt cgacgagctg
     1441 acggatcccg aagcgagggt cctcgtagat atcccggcgg ggaccgcgtc gcaaggcgtc
     1501 acgccgtgac gccccagccg cgttcacaac ctccgcagaa ccggttcaca ggtccttcaa
     1561 aggactcggg tagcctcaag agcgacagag tgagtgagca ccgcggccgt cggcccggcg
     1621 ctccgaagtc aaaggaaggt gcagatatga agaagtggaa gctcacgtgg atcgtgatgg
     1681 cagtggccgt cgccgtcctc gccctggccg gtgtggcgct ggccgccaca gacgcgccga
     1741 gccccgccgc gacccccggc gccggcgtcg cctgcggcgt gacggacgat cctgaggccc
     1801 tcgccgaact gcaggcgctg cgcaccgact tctgggacgc gcgtcaggcc tggttcgaca
     1861 aatacggcgc cgagcgcacg agcgacgagg cgcaggccgc cttccagaag ctgcgcgacg
     1921 accgcatcgc caagacgcag gcggtcttcg acaagtacgg catcgacgcg acggccggtt
     1981 cccgcgccgg cggcggctac ggccgcggcg cgatgatggg cggcggctac ggcaatggcg
     2041 gcatgatggg cggcggctac ggcaacggcg gcatgatggg cggcggctgc ggaggctgcg
     2101 ccctcagcgg caccgactga ccggacaagc tccttcacgt cgggaaccag tgacgccggc
     2161 cgggcgggcc catgaggctc acccggccgg cgtccttgta gctcagtgag tccaggaccc
     2221 ggcttccgcc ttctcggtcc ggacctccag caggatcttg acaccgccgc ggatgacgac
     2281 cacggcgatg gcggcgccga tcacgaggtc ggggatgcgc gaaccgagca gccagaccag
     2341 caccccggcg acgatgacgc cgatgttcgc gatgacgtcg ttggccgaga agatccagga
     2401 tgcccgcatg tggatgccgc cctcgcggtg cttggagatg agcgcgaggc aggtcaggtt
     2461 cgccgcgagc gcgacgagcc cgatgccgac gatgaggagg ctcaccggct cgctgccggc
     2521 caggagccgg cgcgcgacgt ctgccacgac cagcaggccg agcgcgatct gcaggacccc
     2581 gctcacggcg gcggcgttcg attgcaggcg ccgcgagcgt ccgacggcag agagagcgat
     2641 cgcgtacacg aaggcgtccg cgagcatgtc gagcgagtcg gccaccaggc cggtcgcctg
     2701 gccgatccac ccggccactg cctcgacgac gaacagggcg ccgttgatgc cgagcagcac
     2761 caggagcgtg cgccgctcca gctcagccgc ctcgtcgtac tcgttctcgt cagctcccat
     2821 gcccctccgg caggagtgag cctgcgccgc gccgccgcca gtctagcgtc cccgacccgg
     2881 agacgcctga gggcagggtg gtcgactggg acctgtgccc ggctcgcgcg cgtccgggcg
     2941 ccgacctgct atctcagcgt cttcaggtag gcgagcagat cgctccattc ggagccggtc
     3001 agctgccact gcggcatcgg cgcgcctcca ggcggctgcc ctcggggtcg aggccctcga
     3061 tgacggccgt gcgcagggtg gcgtccgaga acgtgggccc acgaccgccg tccggctcca
     3121 gcatgccccg tgggtccgtc aggttgtcgt aggtgatgtt gggcgcggtg aacgccgcgg
     3181 tcgtccgtcc gcggccgtcg gtgccgtggc aggtcgcaca gccgccaccc atcatgccgc
     3241 cttcgcccat catgccgccg ccgctcgagc tgcgtgggat gaggtcgccg tcggcatcga
     3301 ccccggactg gaagatggcc tcgcctctgg ataccggcga caggtctgcc gaacctcggg
     3361 agcccagcgc gaagatgccg accgtcgcca gcgcggcgac ggcgagcgcg accacgacga
     3421 tcacgaggtc actgccgcgc gcacgagcca tgctagaggt cgccgagcct ctgcaggtac
     3481 tcgtcgcggt cgatctcgcc ggcggcgtag cggcgcttga ggatgtcctt gggcgactc
//
LOCUS       DASUMX010000259         6503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1241738, whole genome shotgun sequence.
ACCESSION   DASUMX010000259 DASUMX010000000
VERSION     DASUMX010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 6503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6503
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1241738"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..839)
                     /locus_tag="VFZ86_12265"
     CDS             complement(144..839)
                     /locus_tag="VFZ86_12265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HEX5643093.1"
                     /translation="MAFAEEMERAHGVSGELVLRRGPAGLEIVANGVFLVSAANAASS
                     RALAAAAWPHVAGGALDVLIGGLGIGDALDEALACERVASVTVAELEPTIVRWFRAHG
                     GEHSRRAEAAERCGRARVVVADVADILTAARGAWDVVALDTDNGPEWLVRGENARLYT
                     AAGLHAVRAALRPGGAAVFWSPEEYPSFEGDLRDVFGDVRTVAAVDVIDGRPLDSTMY
                     VACRGDGCPAARE"
     gene            complement(894..2312)
                     /locus_tag="VFZ86_12270"
     CDS             complement(894..2312)
                     /locus_tag="VFZ86_12270"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HEX5643094.1"
                     /translation="MRPTFEYLSPTEKQFVHEQTVRLLEEVGVAYNTPTLTRLLREAG
                     ATVDEGRLRAKLPWELVERCLRQVPRDILLAGRDPACDCTVGAGGMLYTSDGAATYML
                     DDLTGERHEGTEADLAAMMRLYDAVPEIDFTWATITPGDVDARVGNLEMARITFENSR
                     KHLQDEVRTPDQAPVMIELLEAIAGAPLAERPIWSATNCTIAPLQHDAEMTEAHILMA
                     KAGCPIFVLPMPQMGTTGPMSVLGTSILNMAELLSAVVLFQLAVPGCAMVSAVGSAVA
                     EMRSGLYLAGAPEDGLINVVCIEMSRFYGLRTQGSAVSCDAKACDLQAGAEGMLTGLA
                     SALAGADIMLAFGLMDGAQTASLAKTVLDADTVNAIERFMREDPVDEARALIDDLVEV
                     GIGGHFLARRSTRQLYRAGELWQPRLWHRGTFDQYIGTPLVKDAWDRAQALIAENDVP
                     PLPEDVRRHVRAVIDAYARGRD"
     gene            complement(2329..2805)
                     /locus_tag="VFZ86_12275"
     CDS             complement(2329..2805)
                     /locus_tag="VFZ86_12275"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5643095.1"
                     /translation="MSEIGGGEVVIGPLEDLAAMRRLGVACGLEDDGHGEEGIVAAWG
                     ARDGDELVGALALERRSGLDIPNWLAVAESHRRRGIAAALYAALESEARARGVGRLWV
                     TARAPVFFVAQGFVPAPPGKERDILLGGCLDCVQFGVECEPKALTKRLDDTERHDP"
     gene            complement(2802..4262)
                     /locus_tag="VFZ86_12280"
     CDS             complement(2802..4262)
                     /locus_tag="VFZ86_12280"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HEX5643096.1"
                     /translation="MARARLTYLSADDREFVHEQALRVLAEVGVGYNTPEAIDLLAEA
                     GAQVDRSRLTARVPQELVEGCLATCPSEVRLAGRDPRHDVVVGDGSLTFCADGTATYV
                     LDDLTGRREDGSAEWLRRTMRLYDALPEVDYAWPTISARDLDPLTAGLEIEAISLANL
                     TKHVQDEVRDPAHAAPLIEIFEAVAGGTLWDRPVFSTIDCTVAPLQHEREMTQATLAM
                     VRAGVPLLALPMPLSGTTAPMTVLGTMIVNVAELLSAVVLFQLAQPGCGIISGMGAGV
                     ADMRSGLYLCGTPECALMNVIGIEMSRFYGLPCTGSAITCDAKASNQQAGAEGMLTGA
                     ACALAGADTILAFGCSDGAQSLSFAKVLLDCDSVGALRRLVREDPIDATRALLDDIAA
                     VGIGGHYLGRKSTRRFHHGGEIWQPALWQRGPFEAYEGRPLVAEAAARAEELLGAHEV
                     APLSEDVAAEVDAVITRYARSVGAPARRVSWKGGDP"
     gene            complement(4270..5688)
                     /locus_tag="VFZ86_12285"
     CDS             complement(4270..5688)
                     /locus_tag="VFZ86_12285"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HEX5643097.1"
                     /translation="MARARITYLSAVEKEFVHEKTLEVLEKVGVAYNTPRATDLLAAA
                     GARVDRAALTARLTWDVIEAALRTVPRTVLLAGRDPSQDRLLGGGRLIATSDGMTTHM
                     LDDVTGERRGGTTADLADVTRLCDALEEIDTLWPSPNPGDAPADLLPLVTQATMVRNS
                     TKHIQDEVRTPEMVEPILEIYEAACGAPLTERPCFSVTNCTIAPLQHDREMTEAGLKL
                     VERGVPIFVLPMPQAGTTGPMTLLGTCILNMAELLSGIVLYQAAHPGCALVSGVGSAV
                     ADMRSGGYIAASPEIGLINLICLEMSRFYGLPTQATGMSADAKAADFQAGSEGGMTAL
                     VAALAGADSLIAGGGLDGVQISSFAKYVLDNDQIGALRRYLREEPIDETTALMDDILE
                     VGIGGHFLGRRSTRQHSRTEIWRPRAFQRGTFEEFIGRPLAHEAAARAREILETHEVP
                     PLDEAADRHIDGVIGRWAARAV"
     gene            complement(5906..6022)
                     /gene="rrf"
                     /locus_tag="VFZ86_12290"
     rRNA            complement(5906..6022)
                     /gene="rrf"
                     /locus_tag="VFZ86_12290"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(6108..>6503)
                     /locus_tag="VFZ86_12295"
     rRNA            complement(6108..>6503)
                     /locus_tag="VFZ86_12295"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          928 a         2396 c         2230 g          949 t
ORIGIN      
        1 tcctcgaaga tgtgcagcgg gacgacctcg cccggcagca gcacgatgcc gagcgggaag
       61 agccccagca gccggtcgtc agcgtcaccc acgtcgcgcc tcccggttcg cctcgccgcg
      121 cgcgcggccg gacgaccctc agtctactcc ctcgcggccg ggcatccgtc acccctgcag
      181 gccacgtaca tcgtggagtc gagcggccgt ccgtcgatca cgtccacggc cgccaccgtc
      241 ctgacgtcgc cgaacacgtc cctgaggtcg ccctcgaagc tcggatactc ctcgggagac
      301 cagaagacgg ccgcgccgcc gggacggagg gcggcgcgga ccgcgtgcag gccggcggcc
      361 gtgtacaggc gggcgttctc cccgcgcacg agccactccg gcccgttgtc ggtgtccagg
      421 gcgacgacgt cccaggcgcc gcgcgccgcg gtcaggatgt cggccacgtc ggcgacgacc
      481 acgcgcgccc ggccgcagcg ctccgcggcc tcggcgcgcc gcgaatgctc gccgccgtgc
      541 gcgcggaacc agcgcacgat cgtcggctcg agctccgcga cggtcaccga ggccacgcgc
      601 tcgcaggcca gcgcttcgtc gagcgcgtcg ccgatgccga ggccgccgat gagcacgtcg
      661 agagcgccgc cggccacgtg cggccacgcc gccgcggcga gcgcccggct gctggcggcg
      721 ttggcggcgg agacgagaaa gacgccgttg gcgacgatct cgagccccgc cggtccgcgg
      781 cgcagcacca gctcgccgct gacgccgtga gcgcgctcca tctcctccgc gaacgccatg
      841 cgacgaatcc taggggagcg ccccggccgg cgcgaccgga ggaccgggcg ggctcagtcc
      901 cggccgcgcg cgtaggcgtc gatgaccgcg cgcacgtgcc gccggacgtc ctccggcagc
      961 ggcggcacgt cgttctccgc gatcagagcc tgcgccctgt cccacgcgtc cttgaccagc
     1021 ggcgtgccga tgtactggtc gaaggtgccg cggtgccaca ggcgcggctg ccagagctcc
     1081 ccggcacggt acagctgccg cgtgctgcgg cgggccagga agtgaccgcc gatgcccacc
     1141 tccaccaggt cgtcgatgag cgccctggcc tcgtccaccg gatcctcgcg catgaagcgc
     1201 tcgatggcgt tgacggtgtc cgcgtccagc accgtcttcg ccaggctcgc cgtctgggcg
     1261 ccgtccatga gcccgaaggc gagcatgatg tccgcgcccg ccaaggcgct cgcgaggccg
     1321 gtcagcatgc cctcggcgcc ggcctgcagg tcgcacgcct tggcgtcgca gctgacggcg
     1381 ctgccctggg tgcgcaggcc gtagaagcgg ctcatctcga tgcagacgac gttgatgagc
     1441 ccgtcctccg gcgcccccgc gaggtacagc ccgctgcgca tctcggcgac cgccgacccg
     1501 acggccgaca ccatcgcaca acccgggacc gccaattgga agaggacgac cgcgctgagc
     1561 agctcggcca tgttcaggat gctggtgccc aggacgctca tggggccggt ggtgcccatc
     1621 tgcggcatgg gcagcacgaa gatggggcag ccggccttcg ccatcaggat gtgcgcctcg
     1681 gtcatctcgg cgtcgtgctg gagcggggcg atcgtacagt tggtggccga ccagatcggg
     1741 cgttcggcca gcggggcgcc ggcgatcgcc tccaggagct cgatcatcac cggcgcctgg
     1801 tcgggcgtgc gcacctcgtc ctgcaggtgc ttgcgcgagt tctcgaacgt gatgcgcgcc
     1861 atctcgaggt tgccgacccg cgcatcgacg tcgccgggcg tgatggtggc ccaggtgaag
     1921 tcgatctcgg ggacggcgtc gtagaggcgc atcatcgcgg cgaggtcggc ctcggtaccc
     1981 tcgtgccgct cgccggtcag gtcgtcgagc atgtaggtcg ccgcgccgtc gctggtgtag
     2041 agcatcccgc cggcgccgac ggtgcagtcg caggccggat cgcgcccggc cagcaggatg
     2101 tctcgcggca cctgacgcag gcagcgctcg accagctccc acggcagctt cgcccgcagc
     2161 cggccctcgt cgacggtggc gccggcctcc ctgaggagtc tcgtgagggt cggcgtgttg
     2221 tacgccacgc cgacctcctc cagcaggcgc accgtctgct cgtggacgaa ctgcttctcg
     2281 gtcgggctca gatactcgaa cgtcggtcgc acaaggctcc ctccaccgtc acgggtcgtg
     2341 acgctccgtg tcgtccagtc gcttggtcag ggccttcggc tcgcactcga ccccgaactg
     2401 gacgcagtcc agacaccctc cgagcaggat gtcgcgctcc ttgcccggcg gcgccggcac
     2461 gaacccctgg gcgacgaaga agaccggcgc ccgcgcggtc acccacaacc ggccgacgcc
     2521 ccgcgcacgc gcctcgctct cgagcgcggc gtacaacgcc gcggcgatgc cgcggcgccg
     2581 gtgactctcg gccacggcga gccagttggg gatgtccagg ccggagcgcc gctcgagcgc
     2641 aagggcgccg acgagctcgt cgccgtcgcg ggcgccccac gccgcgacga tcccctcttc
     2701 gccgtggccg tcgtcctcga gcccgcaggc cacgcccagc ctgcgcatcg ccgccaggtc
     2761 ctccagcggc ccgatgacga cctccccccc accgatctcg ctcatggatc cccacccttc
     2821 cagctcacgc gccgggccgg tgcgccgacg ctgcgcgcgt agcgtgtgat gaccgcgtct
     2881 acctccgcgg ccacgtcctc ggacagcggc gcgacctcgt gcgcgcccag caactcctcc
     2941 gcgcgggcgg cggcctccgc cacgagcggc cgcccctcgt acgcctcgaa cggcccgcgc
     3001 tgccacagcg ccggctgcca gatctcgccg ccgtggtgga accgccgcgt gctcttgcgc
     3061 ccgaggtagt ggccgccgat gcccaccgcc gcgatgtcgt ccaacagcgc ccgcgtggcg
     3121 tcgatcgggt cctcccgcac cagccgccgc agcgcgccga cggagtcgca gtccagcagc
     3181 accttggcga agctcagtga ctgggcgccg tccgagcagc cgaaggccag gatcgtgtcg
     3241 gcgccggcca gcgcgcacgc ggcgcccgtc agcatgccct cggcgcccgc ctgctggttg
     3301 ctcgccttgg cgtcgcaggt gatggcgctg ccggtgcacg gcagtccgta gaaacggctc
     3361 atctcgatgc cgatcacgtt catgagcgcg cactcgggcg tgccgcacag gtagagccca
     3421 ctgcgcatgt cggcaacgcc cgcgcccatc cccgagatga tgccgcatcc gggctgggcg
     3481 agctggaaga gcacgaccgc gctgagcagt tcggcgacgt tgacgatcat ggtgcccagc
     3541 accgtcatcg gcgccgtggt cccgctcagc ggcatcggca gcgccagcag cggcacgccg
     3601 gcgcgcacca tcgccagtgt cgcctgcgtc atctcccgct cgtgctggag cggcgccacc
     3661 gtgcagtcga tggtcgagaa aacgggccgg tcccagaggg tgccgccggc cacggcctcg
     3721 aagatctcga tgaggggggc cgcgtgcgcc gggtcgcgga cctcgtcctg gacgtgcttg
     3781 gtgaggttcg ccagggagat ggcctcgatc tcgaggcccg cggtgagcgg gtccagatcg
     3841 cgggccgaga tggtcggcca ggcgtagtcc acctccggga gggcgtcgta caggcgcatc
     3901 gtgcgccgca gccactcggc ggagccgtcc tcgcggcgcc ccgtcaggtc gtcgagcacg
     3961 taggtcgcgg tgccgtcggc gcagaaggtg agcgacccgt cgcccacgac gacgtcgtgc
     4021 cgcgggtcgc gcccggccag ccgcacctcc gaagggcagg tcgcgaggca gccctcgacg
     4081 agctcctggg gcaccctggc cgtgagcctc gacctgtcca cctgggcgcc cgcctcggcc
     4141 agcaggtcga tcgcctccgg cgtgttgtag ccgacgccga cctcggccag cacgcgcagc
     4201 gcctgctcgt ggacgaactc cctgtcgtcc gccgagaggt aggtcaggcg tgcgcgcgcc
     4261 atcgcccgct cagaccgcgc gcgcggccca gcggccgatg acgccgtcga tgtggcggtc
     4321 ggcggcctcg tccagcggcg ggacctcgtg cgtctcgagg atctcccgcg cgcgggcggc
     4381 ggcctcgtgc gcgaggggcc gcccgatgaa ctcctcgaac gtcccgcgct ggaacgcccg
     4441 cggccgccag atctcggtcc gggagtgctg ccgcgtgctc ctgcgcccca ggaagtgccc
     4501 gccgatgccg acctcgagga tgtcgtccat gagcgcggtc gtctcgtcga tgggctcctc
     4561 gcgcaggtag cggcgcagcg cgccgatctg gtcgttgtcg agcacgtact tcgcgaagct
     4621 cgagatctgc acgccgtcca gacctccgcc ggcgatgagc gagtcggcgc cggcgagggc
     4681 ggccacgagc gccgtcatgc cgccctccga gcccgcctgg aagtcggccg ccttggcgtc
     4741 ggccgacatg ccggtggcct gcgtgggcag cccgtagaag cggctcatct cgaggcagat
     4801 caggttgatg agcccgatct ccgggctggc ggcgatgtac cctccggagc gcatgtcggc
     4861 gaccgcggag cccaccccgg agacgagggc gcagccgggg tgcgccgcct ggtagagcac
     4921 gatccccgag agcagctcgg ccatgttcag gatgcacgtg cccagcaggg tcatcggccc
     4981 cgtggtcccg gcctgcggca tgggaaggac gaagatgggc acgccgcgct cgacgagctt
     5041 gagcccggcc tccgtcatct ccctgtcgtg ctgcaggggt gcgatggtgc agttcgtgac
     5101 cgagaagcac gggcgttccg tgagcggcgc cccacacgcg gcctcgtaga tctccaggat
     5161 cggctcgacc atctccggcg tgcgcacctc gtcctggatg tgcttggtgg agttgcgcac
     5221 catcgtggcc tgcgtgacca gcgggagcag gtccgcgggg gcgtcccccg ggttgggcga
     5281 cggccacagg gtgtcgatct cctcgagggc gtcgcacagc cgcgtgacgt cggcgaggtc
     5341 cgccgtggtc ccgccgcgtc gctcgccggt gacgtcgtcg agcatgtgcg tggtcatgcc
     5401 gtcgctggtg gcgatgaggc gcccgccccc gaggaggcga tcctgggagg ggtcgcggcc
     5461 ggcgagcagg acggtccggg ggaccgtcct gagcgcggcc tcgatcacgt cccaggtgag
     5521 gcgcgcggtc agcgctgcgc ggtcaacgcg cgccccggcc gccgcgagca ggtcggtggc
     5581 cctcggcgtg ttgtacgcga cgcccacctt ctccagcacc tcgagcgtct tctcgtggac
     5641 gaattccttc tccacagcgg agaggtaggt gatgcgggcc ctggccacgg cgcgtcctcc
     5701 cttgggtacc agacaggcta ccacagcctc acggagcagc tcaggaaatc gcacggccca
     5761 tgcgaggttt gtcacacaat ccgtcgcagt ctgagtgagg attgcgaact ccgcagcgca
     5821 cgaccgccca gctctccgcc cgtttcgtcc ccgttaacgc cgagagcccg acgcgccttt
     5881 cggcggtcgg gctctcgaag caaaaatccg gcggcgtcct actctcccgc ggggtcgccc
     5941 ccgaagtacc atcggcgctg gagggcttaa cttctctgtt cggaatggga agaggtgtga
     6001 cccctccgct atggccgccg aaaacctggc gccgtcacac tggcgcacaa acattcgtgc
     6061 cggcacgcgg acacggacct gaaaactgca gagcggcatt ggagttcaaa gtcaagccct
     6121 cgggcaatta gtactggtct gctgaacaca ttgctgtgct tacacatcca gcctatcaac
     6181 caggtggtct acctgggccc ttacccattc tagatggtgg gagatctcat ctcgaggcgc
     6241 gcttcccgct tagatgcttt cagcggttat cgcttccaga cgtagctaac cagcagtgcc
     6301 gttggcacga caactgatac accagaggtc tgttcacccc ggtcctctcg tactaggggc
     6361 aacccctctc aaatctccaa cgcccacaga agatagggac cgaactgtct cacgacgttc
     6421 tgaacccagc tcacgtaccg ctttaatggg cgaacagccc aacccttggg acctacttca
     6481 gccccaggat gcgatgagcc gac
//
LOCUS       DASUMX010000260         4255 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1098456, whole genome shotgun sequence.
ACCESSION   DASUMX010000260 DASUMX010000000
VERSION     DASUMX010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4255
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1098456"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="VFZ86_12300"
     CDS             complement(<1..542)
                     /locus_tag="VFZ86_12300"
                     /inference="COORDINATES: protein motif:HMM:NF015686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEX5643098.1"
                     /translation="MDVLLDPVNLLALLTLTLLEIVLGIDNVIFIAIITDSLPPERRN
                     FARRLGLGLALFGRVALVLGVSWLLSLEHPLFTVLEHEVTISGLILIGGGLFLLYKAV
                     REIFNTTELHEEEHGEVRRRASVAGVIAQIVVIDMIFAIDSVLTAVGMTSEVLLIIVA
                     ITIAIAVMMFYAAPVSDFISR"
     gene            complement(626..1423)
                     /locus_tag="VFZ86_12305"
     CDS             complement(626..1423)
                     /locus_tag="VFZ86_12305"
                     /inference="COORDINATES: protein motif:HMM:NF022599.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2927 domain-containing protein"
                     /protein_id="HEX5643099.1"
                     /translation="MPVRLIGRVFSGLFLLLVLAAALYGAWMALDWVDDRNGFGLLDR
                     QPDQPLVFSSLNRYTKEAAGKASFRRYFTTMVVSQPDAGMTDPPVVTKWERPRVVVKL
                     LNSGGPGVASYLRQLVARLNRMQGEVRFAVGDVAPRITVRFLSHEAYARAIGDGSVGS
                     TRTRYYRSSPGMIRASITIDAGRQGSPGQIKATLIHELTHAIGGGGHFTSPSYSRRSV
                     LYEASQVTSWSQNDAAVIRILYSPWIESGMTAEEARASLRRYARSAD"
     gene            complement(1482..1943)
                     /locus_tag="VFZ86_12310"
     CDS             complement(1482..1943)
                     /locus_tag="VFZ86_12310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HEX5643100.1"
                     /translation="MEPVQHIRILQHAYAAQLADATTQYGKAGILEQVTETRRAARLS
                     SGAATAAQMGVTEPAAAFTTSAGLFGCADWAVAPDDDGFVATASRCLLCALVKQMGGP
                     SPCRLFCLDPIEGMIVGLAPDAAVDVRETLYDGGACRVRVRARVEAPAPAV"
     gene            complement(1988..2728)
                     /locus_tag="VFZ86_12315"
     CDS             complement(1988..2728)
                     /locus_tag="VFZ86_12315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5643101.1"
                     /translation="MTTRAATVPSPPDPATLVADYGRLVSSLCWRMTRDREVAREAAQ
                     EVWLAVLEGLPRFRGEASVSTWIYAIARRVIGGYAAAQRTYSVRFLSTYFEGEGEPAP
                     PEIPDLDHDLWVRSMCDQCLCGMLQCLEPDPRLAFLLRDIAGLGYDDVARVLEVEPAA
                     ARQMVSRARRKLDRFLKGHCALAHPDGPCRCRMRRRVEAIDLPAEYARLRVAAGRARV
                     FKDSEQVLPAKDYWLELSQTAIQGHQPQ"
     gene            3014..3514
                     /locus_tag="VFZ86_12320"
     CDS             3014..3514
                     /locus_tag="VFZ86_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007271268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HEX5643102.1"
                     /translation="MDTDILITVLAGVLLVVAAVGTIYPILPGSLIAIVTLLLWAWAL
                     GSWPAWVAAVLGALICVVGWSASAVLTGRKLKQLEVPRWSILVAVVGGVTGMFLIPVV
                     GLFIGFGVALLLSEWVRHGDLRQAWHYSVQTLKATGTGVLVEFLMVCVAAAVWTVGAF
                     AHFWVG"
     gene            complement(3524..3697)
                     /locus_tag="VFZ86_12325"
     CDS             complement(3524..3697)
                     /locus_tag="VFZ86_12325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643103.1"
                     /translation="MDRIVSIDHIAGSARDENVRAERLRHRRAAQERCRRAGLLRRCT
                     PVLPDGTGCCRDA"
     gene            complement(3724..>4255)
                     /locus_tag="VFZ86_12330"
     CDS             complement(3724..>4255)
                     /locus_tag="VFZ86_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5643104.1"
                     /translation="LSARRVGPTGKAYGLDMTDEMLALARRNQRKAGATNVEFLKGEI
                     EDIPLPDASVDVIVSNCVINLSVDKDRVLAEAFRVLRPGGRFAVSDIVFQGDVSLIPD
                     TVMRDIEAWAGCVAGALEEQDYLAKLRRAGFADPSIEVTQVYAADRELCGVDLSDIPQ
                     HVRVVSGFVRAVKPGA"
BASE COUNT          606 a         1468 c         1543 g          638 t
ORIGIN      
        1 cggctgatga agtccgacac cggcgccgcg tagaacatca tcacggcgat cgcgatggtg
       61 atggcgacga tgatcagcag cacctcgctg gtcatgccga ccgccgtcag caccgagtcg
      121 atggcgaaga tcatgtcgat gacgacgatc tgcgcgatca cgcccgccac cgaggcgcgc
      181 cggcgcacct cgccgtgctc ctcctcgtgc agttccgtcg tgttgaagat ctcgcgcacg
      241 gccttgtaaa gcaggaagag cccgccaccg atcaggatca ggccgctgat ggtgacctcg
      301 tgctcgagca cggtgaacag cgggtgctcc agcgacagca gccacgacac gccgagcacc
      361 agggcgacgc ggccgaagag cgcgaggccg agtcccaggc gccgggcgaa gttgcggcgc
      421 tcgggcggca gcgagtcggt gatgatggcg atgaagatga cgttgtcgat gccgaggacg
      481 atctccagca gcgtcagcgt gagcagcgcg agcaggttca cggggtccag aagcacgtcc
      541 atgcgccatc tccttgggat cgcctgccag tgctcttacc cgcacgacga cgggtagccg
      601 cctcgaagcg tcgccgcgga tgccgtcagt ccgcggagcg cgcgtagcgg cgcagcgagg
      661 cccgggcctc ttccgccgtc atgcctgatt cgatccacgg agagtacagg atgcggatga
      721 cggcggcgtc gttctggctc caggaggtga cctggctcgc ctcgtagagg acgctgcggc
      781 ggctgtagga cggggaggtg aagtggccgc cgccgccgat cgcgtgggtc agctcgtgga
      841 tcagggtggc cttgatctgg cccgggctcc cctggcgccc cgcgtcgatc gtgatgctcg
      901 ccctgatcat cccgggcgac gaccggtagt accgcgtccg cgtgctgccg acgctgccgt
      961 cgccgatggc gcgcgcgtag gcctcgtggc tcaggaagcg gacggtgatg cgcggcgcca
     1021 cgtcgcccac cgcgaaccgc acctcgccct gcatgcggtt caggcgcgcg accagttgcc
     1081 gcaggtagga cgcgacgccg ggcccgccgc tgttgagcag cttgacgacg acgcgcgggc
     1141 gctcccactt ggtcaccacc ggcgggtccg tcatgccggc gtccggctgc gacaccacca
     1201 tggtggtgaa gtagcggcgg aacgaggcct tgcccgccgc ctccttggtg tagcggttga
     1261 gggaggagaa gaccagtggc tgatccggct gccggtccag caggccgaag ccgttgcggt
     1321 cgtccaccca gtcgagtgcc atccaggcgc cgtacagggc ggccgccagg acgagcagca
     1381 ggaacaggcc ggagaagacg cggccgatca gacgcacggg cactcacctc cttctcccag
     1441 tttgcccacc agggagggga cgatgccgcg ggcgccggcg ttcagacggc cggcgccggc
     1501 gcctcgacgc gcgcccggac gcgcacgcgg caggcgccgc cgtcgtagag ggtctcccgg
     1561 acgtcgacgg cggcgtccgg cgcgagcccg acgatcatgc cctcgatggg gtcgaggcag
     1621 aagaggcggc acgggctggg gccgcccatc tgcttgacca gggcgcagag caggcagcga
     1681 gaggcggtgg cgacgaagcc gtcatcgtcc ggcgccaccg cccagtcggc gcagccgaag
     1741 agccccgccg aggtggtgaa ggcggccgcg ggctcggtca cgcccatctg tgcggccgtc
     1801 gccgcgcccg acgagagacg ggcggcgcgg cgggtctcgg tcacctgctc gaggatgccc
     1861 gccttgccgt actgcgtcgt cgcgtccgcg agctgggcgg cgtaggcgtg ctgcaggatg
     1921 cggatgtgct ggacgggttc catctgagcc tccatcgtgt cgccgccctc cccgtctggg
     1981 agggccgtca ctgaggttgg tggccctgga tggcggtctg tgacaactcg agccagtagt
     2041 ccttggccgg cagcacctgt tccgagtcct tgaagacgcg cgcccggccg gcggcgacgc
     2101 ggagccgggc gtactcggcc ggcaggtcga tggcctccac ccgccggcgc atgcggcagc
     2161 ggcaggggcc gtcggggtga gccagggcgc agtgcccctt gaggaaccgg tcgagcttgc
     2221 gccgcgcgcg ggagaccatc tggcgcgcgg cggcgggctc gacctcgagc acccgcgcga
     2281 cgtcgtcgta gcccaggccg gcgatgtcgc gcagcaggaa cgcgaggcgc gggtccggct
     2341 cgaggcactg cagcatgccg cacaggcact ggtcgcacat cgagcgcacc cagaggtcgt
     2401 ggtccaggtc ggggatctcc ggcggcgcgg gctcgccctc accctcgaag taggtgctga
     2461 ggaagcgcac gctgtacgtg cgctgcgccg ccgcgtaccc gccgatcacg cgccgcgcga
     2521 tggcgtagat ccaggtggag acgctcgcct cgccgcgaaa tcggggcagc ccctccagca
     2581 ccgcgagcca cacctcctgg gcggcctcgc gcgcgacctc gcggtcgcgc gtcatgcgcc
     2641 agcagagcga cgagacgaga cggccgtagt ctgcgacgag cgtcgctggg tcggggggcg
     2701 acgggactgt ggcggctctg gtggtcacac gtggctcgct ggagaggggc gccttcggcg
     2761 aggtcggttc gcattgtagc agcgcccttc atacgaaacg ggcgggccgg cctcacggcc
     2821 ggcccgcccg ccttgcgttc acctccggcc tcagaacacg aaggccagaa ggatgacgat
     2881 gagtatgatg gccagtacgg cgccaagccc tatgtacatg ggaaccctcc ttctgtctcg
     2941 cacccagggt cttccccgca cacaggttcc ctgtcggctc tgccgttagg gccgcttgtg
     3001 gcaccatagc gcggtggaca ccgacatcct catcaccgtg ctcgcggggg tcctgctggt
     3061 cgtggccgcc gtcgggacga tctaccccat cctgccgggc agcctcatcg cgatcgtcac
     3121 gctgctgctc tgggcctggg ccctgggctc gtggccggcg tgggtcgcgg ccgtcctcgg
     3181 ggcgctcatc tgcgtggtgg gctggtcggc cagcgccgtg ctcaccggcc gcaagctcaa
     3241 gcagctggag gtgccacgat ggtccatcct cgtggccgtc gtcggcggcg tgaccgggat
     3301 gttcctgatc cccgtggtcg gcctgttcat cgggttcggc gtcgccctgc tcctgagcga
     3361 gtgggtgcgg cacggcgacc tgcgccaggc ctggcactac agcgtgcaga cgctcaaggc
     3421 caccgggacc ggcgtgctcg tcgagttcct gatggtgtgt gtggccgccg cggtgtggac
     3481 ggtcggcgcg ttcgcccact tctgggtggg gtaggcgcgc ggctcaggcg tcgcggcagc
     3541 agcccgtgcc gtccggcagc accggcgtgc agcggcgcag gaggccggcg cgcctgcagc
     3601 gctcctgcgc cgcgcgccgg tgccgcagcc tctccgccct gacgttctcg tcgcgggccg
     3661 agcccgcgat gtggtcgatg ctcacgatgc gatccatgac tcctccgtct tctccgcgtc
     3721 tcgtcaggcg cccggcttga cggcgcgcac gaagccgctg accacgcgca cgtgctgcgg
     3781 gatgtcgctg aggtccacgc cgcagagctc tcggtccgcg gcgtacacct gcgtgacctc
     3841 gatcgagggg tccgcgaagc ccgcacgccg cagcttggcc aggtagtcct gctcctccag
     3901 cgcgcccgcg acgcaccccg cccacgcctc gatgtcgcgc atgaccgtgt cggggatgag
     3961 gctcacgtcg ccctggaaga cgatgtccga gacggcgaac cgcccgcccg gcctgagcac
     4021 acggaacgcc tccgcgagga ctctgtcctt gtccacggag aggttgatga cgcaattgct
     4081 gacgatcacg tcgacggacg cgtccggcag cggaatgtcc tcgatctctc ccttgaggaa
     4141 ctcgacgttg gtcgcgccgg ccttgcgctg gttgcgacgc gccagagcga gcatctcatc
     4201 ggtcatgtcg agcccgtagg ccttgccggt ggggccgacg cggcgggcgg agagc
//
LOCUS       DASUMX010000261         7026 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_237075, whole genome shotgun sequence.
ACCESSION   DASUMX010000261 DASUMX010000000
VERSION     DASUMX010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 7026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7026
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237075"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(364..963)
                     /locus_tag="VFZ86_12335"
     CDS             complement(364..963)
                     /locus_tag="VFZ86_12335"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015173025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEX5643105.1"
                     /translation="MAFELPPLPYAEDALEPDYTARTISFHYGKHHKTYVDTLNKLVA
                     GTDLEGKSLEDVIMAVAGKPDKQAVFNNAAQVWNHTFFWSCMKPGGGGAPAGPIAEKI
                     EELGGWEKFVEDFKAAAVGRFGSGWAWLVADNGDVKIVSTPNAEMPAAPGVTPLLVVD
                     VWEHAYYLDYQNLRPVWVQTFFDKLVNWDFVNENLAKAK"
     gene            complement(1033..1428)
                     /locus_tag="VFZ86_12340"
     CDS             complement(1033..1428)
                     /locus_tag="VFZ86_12340"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX5643106.1"
                     /translation="MWHEVAKLDDIAPGGLKYVRAGDDDREICLCEYDGSIYAVSRRC
                     GHENAPLSDGALEGWIVTCPLHNAQFDIRNGKNLSWPIDHDMGPEDEIPEPVQRWFKL
                     EKRLQWKTRVHDLHTYEVRVRDGAIEVDV"
     gene            complement(1431..1757)
                     /locus_tag="VFZ86_12345"
     CDS             complement(1431..1757)
                     /locus_tag="VFZ86_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013195589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016730 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-thioredoxin reductase catalytic
                     domain-containing protein"
                     /protein_id="HEX5643107.1"
                     /translation="MSNEARKESLRYLFDRVVQPLGYKFSPDEELVDELLEAEAKTEQ
                     DLGAPFCPCQGRTGVRAEDMQICCPCIPFHRAHFDAMKRCWCGLFVHLDVTDPDSLPQ
                     FPPDEV"
     gene            complement(1754..2020)
                     /locus_tag="VFZ86_12350"
     CDS             complement(1754..2020)
                     /locus_tag="VFZ86_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEX5643108.1"
                     /translation="MVDEVYVYALSTCPWCRKTKKWFDDSQIAYDFVDVDTLPDEEQD
                     AVADKAYELSGGRRFPVVVINGEVIVGYSPDKFLEHLKGWGGGA"
     gene            complement(2023..2343)
                     /locus_tag="VFZ86_12355"
     CDS             complement(2023..2343)
                     /locus_tag="VFZ86_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012899726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEX5643109.1"
                     /translation="MTGPSWVPTIDDAKVRDGAYVAVFPKGLGVLLARIDGDLFAVRN
                     KCAHMGCPLDGGKLEGPVLTCPCHDWRFDVRTGVFLDAAELALETFPVKVEDGKVHVE
                     VGGG"
     gene            complement(2502..3005)
                     /locus_tag="VFZ86_12360"
     CDS             complement(2502..3005)
                     /locus_tag="VFZ86_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX5643110.1"
                     /translation="MDDPVLSRRSIRRYTDQPVDDDLVERLLRAAMAAPSAGNQQPWR
                     FVVIRDRAMLEAIADMHPYGKMLPGAPVAILVCGDPGDAKWAALWDQDCSACTENLLI
                     EAELLGLGAVWLGIHPLPERETALRELLGIPEHVVPFAVVPFGWPAQRKEPSDRYDAA
                     RVHSERW"
     gene            3359..4846
                     /locus_tag="VFZ86_12365"
     CDS             3359..4846
                     /locus_tag="VFZ86_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5643111.1"
                     /translation="MDTARSHYDAIVVGAGPAGIFAALELVRRNGARVLIVERGPDIS
                     RRSCPARRSGVCAGCEPCDITCGWGGAGAFSDGKLTLTPDVGGWLDTFVGKERLTGLI
                     DYVDGVWIEYGAPEEVHGGGKKFDRIRKEALLHGMTLVESPVRHMGTERSYDILTRMR
                     AELESQVEVLTRSRVDRILTEKSAGESDLRATGVVLEDGTRLTADAVVAAPGRDGAAW
                     LMEECKRLHIAMRTNPVDIGVRVEVSAAVMEPLTTALYESKLIYYTPRFDDPVRTFCM
                     NPYGAVSLENYGDVVTVNGHSYADFKTDRTNFALLVSTDFTEPFDDPIAYGKSIARLA
                     NVLGEDIIVQRLGDLHQGHRSTMDRIRRGTVQPTLQGATAGDLAFVLPYRHLTDILEM
                     LAAMDKLAPGVNGRDTLLYGVEVKFYSARPELDDGLQTPVKNLYAIGDGAGITRGLIQ
                     ASAAGVIAARSILGVRGDGATGEESSGGAGDMAAAGDASAASSIA"
     gene            complement(4868..5995)
                     /locus_tag="VFZ86_12370"
     CDS             complement(4868..5995)
                     /locus_tag="VFZ86_12370"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEX5643112.1"
                     /translation="MERVAAIVLAAGSSSRMGAFKPLLEVRGRTLLERAVGAFTQAGV
                     RDVVVVAGHRRDEVAAAAAGAGARVITNPGYDQGMFSSLRMGTLGLDPGVTRFFVLPA
                     DVPLVRPETIGRLLRQGRVARGGAAAVVYPLSGGVEGHPPLLDGALRAEIRAADPPDG
                     LRELLLGHAAASAVVEVDDPGVLLDADTPEDLDRIRGLAASEGLPDAGRCLRLLAEQG
                     VPRARIDHSLVVTAVATALTTALNVRGQYLIAPLVTAGALLHDVAREQPRHAEAGAAL
                     LERLGYPRVAAVVRLHVRLGERAHDEPDEAQMIYLADKLVQGSSVVGLDARFAARLDR
                     LAGDPAARAAVLERKEEAVHVLRRVEQALGAPVDEVLPAGA"
     gene            complement(5999..>7026)
                     /locus_tag="VFZ86_12375"
     CDS             complement(5999..>7026)
                     /locus_tag="VFZ86_12375"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="XdhC/CoxI family protein"
                     /protein_id="HEX5643113.1"
                     /translation="EQLEAGRAFALVTLVADQGSTPRAAGAEMLVRDDGSIAGSIGGG
                     LIEHTMMTAAATALAERRSRRERADLRGEDLASGEKMVCGGAADVLITFVPPRDAELL
                     AVCRALKDARDAGRRSWYVTASPPGDDAGSFARPVEHCLLAESGELAGAAVCEAAALR
                     RLTAAAALHGTATLPDGRDVVLERVDPPALAVVCGAGHVGCAVAPLLAGLGFRVVVLD
                     DREDLASAERFPGAEVVVRPFDGALAAVGVDERAYVVIVTRGHVHDLDVLVQALRAGA
                     RYVGVMGSRAKRARMLAALRAAGFDEGTIGRVHSPIGVAIGAETPAELAVSIAAELVQ
                     ARAGAGA"
BASE COUNT          980 a         2608 c         2491 g          947 t
ORIGIN      
        1 ggcccctcct gatccttcct tcgccctggt gggcccggtg cgggacgacg cccgcgtgcg
       61 ctggcgcgca cttcccactc accgtcccga aaggacacac cagcgatgga tcacgtacac
      121 gtacggcaac gactccaggc cttcgccgcc cgcctccggc gggcggcagg tccggcgggc
      181 cacgagagct ccggagcgga gcgcgccgcc gcgacccacg aggcctcggt cggcctgcgc
      241 agcatgaaca cgctcggcgc aggccggcgg tgaacccgca cgccgtccgg cgctcagtcg
      301 ccctcgcagg ctgagcgccg gacggccaca ctcctcccca cgccggcggc tacctcgccg
      361 gtgtcacttg gccttcgcga ggttctcgtt gacgaagtcc cagttgacga gcttgtcgaa
      421 gaaggtctgc acccacaccg ggcgcaggtt ctggtagtcg aggtagtagg cgtgctccca
      481 gacgtccacc acgagcagcg gcgtcacgcc cggagccgcc ggcatctcgg cgttcggcgt
      541 gctcacgatc ttgacgtcgc cgttgtccgc gacgagccag gcccagccgg acccgaagcg
      601 gcccacggcc gcggccttga agtcctcgac gaacttctcc cagccgccga gctcctcgat
      661 cttctctgcg atggggcccg ccggcgcgcc accgccgccc ggcttcatgc agctccagaa
      721 gaaggtgtgg ttccagacct gtgccgcgtt gttgaacacg gcctgcttgt ccggcttgcc
      781 ggccacggcc atgatcacgt cctcgaggct cttgccctcg aggtcggtgc cggccaccag
      841 cttgttgagc gtgtcgacgt aggtcttgtg gtgcttgccg tagtggaagc tgatcgtgcg
      901 cgcggtgtag tcaggctcca gcgcgtcttc cgcgtacggg agcggcggca gttcgaacgc
      961 catcgttcct cctgtgagta gtgggacgct ttgcatgacg cttgcggccc cgcggggccg
     1021 ggtggtggcc ggtcagacgt cgacttcgat ggcgccgtcc cgcacgcgca cctcgtaggt
     1081 gtgcaggtcg tgcaccctgg tcttccactg cagccgtttc tccagcttga accagcgctg
     1141 gaccggctcc gggatctcgt cctccgggcc catgtcgtgg tcgatgggcc agctgaggtt
     1201 cttgccgttg cggatgtcga actgggcgtt gtgcaaggga cacgtgacga tccatccctc
     1261 gagcgcgccg tcgctcaggg gcgcgttctc gtggccgcag cggcggctca cggcgtagat
     1321 ggagccgtcg tactcgcaca ggcagatctc acggtcgtcg tcgccggcgc gcacgtactt
     1381 gaggccgccg ggggcgatgt cgtcgagctt cgcgacttcg tgccacatga tcagacctcg
     1441 tccggcggga actggggcag gctgtccggg tcggtgacgt ccaggtggac gaagagaccg
     1501 caccagcagc gcttcatcgc gtcgaagtgg gcgcggtgga acgggatgca gggacagcag
     1561 atctgcatgt cctcggcgcg cacgccggtg cgcccctggc aggggcagaa cggcgcgccg
     1621 aggtcctgct cggtcttggc ctcggcctcg aggagctcgt cgacgagctc ctcgtcgggc
     1681 gagaacttgt agcccagcgg ctggaccacg cggtcgaaca ggtagcgcag cgactccttg
     1741 cgggcctcgt tgctcatgct cctcctcccc agcccttgag gtgctccagg aacttgtcgg
     1801 ggctgtagcc gacgatcacc tcgccgttga tgacgacgac ggggaaccgg cggccgccgc
     1861 tgagctcgta ggccttgtcg gcgacggcgt cctgctcctc gtcggggagg gtgtcgacgt
     1921 cgacgaagtc gtaggcgatc tggctgtcgt cgaaccactt cttggtcttg cggcaccagg
     1981 ggcaggtgct gagcgcgtag acgtagacct cgtcgaccat cctcaccctc cccctacctc
     2041 gacgtggacc ttgccgtctt ccaccttgac ggggaaggtc tcgagcgcca gctcggccgc
     2101 gtcgaggaac acccccgtac gcacgtcgaa ccgccagtcg tggcaggggc aggtgagaac
     2161 aggaccctcg agcttgccgc cgtcgagcgg acagcccatg tgcgcgcact tgttacgcac
     2221 cgcgaacagg tcgccgtcga tgcgcgcaag gagcactccg aggcccttgg gaaagacggc
     2281 gacgtacgcg ccgtcacgga cttttgcgtc gtcaatggtc gggacccagg aaggtcctgt
     2341 cacggctcac cccttcgctc cgcctgcaca gtcgtcgtag gcgacccgtg gggcggggcc
     2401 tgtctcgccg gcgccgggcc ggagggcggc gcccgccgcg tcacgggtcg agtggcaaag
     2461 gtacccgcgc gcgaggaggg acaaactgtc gacgcccgcc gtcaccagcg ctcgctgtgg
     2521 acgcgggccg cgtcgtagcg gtcggaaggc tccttgcgct gcgccggcca gccgaacggg
     2581 acgacggcga agggcacgac gtgctcgggg atcccgagca gctcgcgcag cgcggtctca
     2641 cgctcgggca gcgggtggat gccgagccac acggccccca ggccgagcag ctcggcctcg
     2701 atcagcaggt tctcggtgca ggccgagcag tcctggtccc agagcgcggc ccacttggcg
     2761 tcgcccgggt ctccgcacac caggatcgcg accggcgcgc cgggcagcat cttgccgtag
     2821 ggatgcatgt cggcgatggc ctccagcatc gcgcgatcgc ggatgaccac gaaccgccag
     2881 ggctgctgat tgccggcgga cggcgcggcc atggccgcgc ggagcagccg ctcgacgagg
     2941 tcgtcgtcca cgggctggtc ggtgtacctg cggatgctgc gccggctgag gaccgggtcg
     3001 tccatgctca cctcgggcgt cgcgggaacc tgcaccgcga gcgtacagac ccggcggccg
     3061 ggctggcaaa ccacgtgccc gccggggcgc ggaggcgggg acgggcgggc ggccgtgagg
     3121 ccgcccgccc cgcctggtcc ccgccctcct gccgccgccg cgccggtcct tccccgtcct
     3181 gcgcggcgtc cccctgcggc atgctcgcgg ctcctccgcc gacgagcggc cttgcgccgg
     3241 ggcgcggtga gcggtggcgc ctacgagacg cccggcgatt gtcaggcgtt cgcggcgcgg
     3301 ctatcatctc ggcaagggaa ctgctcgccc ttacgccgac ctccccccct gaacgcctat
     3361 ggataccgca cgctcccact acgacgcgat cgtcgtcggc gccggccccg ccgggatctt
     3421 cgccgcgctc gagctggtcc ggcgcaacgg cgcccgcgtg ctcatcgtcg agcgcggccc
     3481 cgacatcagc cgccgcagct gcccggcgcg caggtccggc gtctgcgccg gctgcgagcc
     3541 ctgcgacatc acctgcggct ggggcggggc cggcgccttc agcgacggca agctcaccct
     3601 cacgccggac gtcggcggct ggctcgacac cttcgtgggc aaggagcgcc tcaccgggct
     3661 catcgactac gtcgacggtg tgtggatcga gtacggcgcg ccggaggagg tccacggcgg
     3721 cggcaagaag ttcgacagga tccgcaagga ggcgctcctc cacgggatga ccctcgtcga
     3781 atcgccggtc cgccacatgg gcaccgagcg ctcctacgac atcctcacgc gcatgcgcgc
     3841 ggagctggag tcccaggtcg aggtgctcac ccgcagccgc gtggaccgca tcctgacgga
     3901 gaagtccgcc ggcgagagcg acctgcgcgc caccggcgtg gtcctggagg acggcacgcg
     3961 gctcaccgcc gacgccgtcg tcgctgcgcc cggacgcgac ggcgccgcct ggctcatgga
     4021 ggagtgcaaa cgcctgcaca tcgccatgcg caccaacccc gtcgacatcg gtgtgcgcgt
     4081 ggaggtctcg gcggcggtca tggagccgct caccacggcg ctctacgaga gcaagctcat
     4141 ctactacacg ccgcggttcg acgacccggt gcgcacgttc tgcatgaacc cctacggggc
     4201 ggtctcgctc gagaactacg gcgacgtcgt caccgtcaac gggcacagct acgccgactt
     4261 caagaccgac cgcaccaact tcgccctgct ggtcagcacc gacttcaccg agccgttcga
     4321 cgaccccatc gcgtacggca agagcatcgc ccggctggcc aacgtcctcg gcgaggacat
     4381 catcgtgcag cgcctcggcg acctgcacca gggccaccgc agcaccatgg accgcatccg
     4441 caggggcacc gtgcagccca cgctgcaggg cgccaccgcc ggcgacctcg cgttcgtgct
     4501 cccgtaccgg cacctcaccg acatcctcga gatgctggcg gcgatggaca agctcgcccc
     4561 cggcgtcaac gggcgcgaca cgctgctcta cggcgtggag gtcaagttct actcggcgcg
     4621 gccggagctc gacgacggcc tgcagacacc ggtcaagaac ctgtacgcca tcggcgacgg
     4681 cgccggcatc acccgcggcc tgatccaggc ctccgccgcg ggcgtgatcg ccgcgcgcag
     4741 catcctcggg gtcagagggg acggcgcgac gggcgaggag tcgtcaggcg gggcgggcga
     4801 catggccgcg gccggcgacg cctcggcagc gagctccatc gcctagaccg gccgggccgg
     4861 gcgtccctca ggcgccggcc ggcagcacct cgtccaccgg cgcccccaga gcctgttcca
     4921 cccggcgcag cacgtgcacg gcctcctcct tgcgctcgag caccgcggcg cgcgccgcgg
     4981 ggtcgccggc aagccggtcc agacgcgcgg cgaagcgcgc gtccaggccg acgacgctgc
     5041 tgccctggac gagcttgtcg gcgagataga tcatctgggc ctcgtccggc tcgtcgtgcg
     5101 cccgctcgcc caggcggacg tggaggcgga cgaccgcggc gacgcgcggg tagccgaggc
     5161 gctcgagcag cgccgcgccc gcctccgcgt gccggggctg ctcccgggcg acgtcgtgga
     5221 gcagcgcccc ggcggtcacg agcggggcga tgaggtactg gccgcgcacg ttgagcgcgg
     5281 tggtgagcgc cgtggccacc gcggtgacga ccagggagtg gtcgatgcgc gccctcggca
     5341 cgccctgctc cgccagcagg cgcagacagc ggcccgcgtc cggcagaccc tcactcgcgg
     5401 ccaatccgcg gatgcggtcg aggtcctccg gcgtgtcggc gtcgagcagc acgccgggat
     5461 cgtccacctc gaccacggcg gacgcggcgg cgtggccgag cagcagctcg cgcagcccgt
     5521 cgggcgggtc ggcggcgcgg atctccgccc gcagcgcgcc gtcgagcagc ggcggatgtc
     5581 cttcgacgcc gccggacagc ggatacacga ccgcagccgc gccaccgcgc gccaccctgc
     5641 cctgcctcaa gaggcgcccg atcgtctccg gccggacgag cgggacgtcc gccggcagca
     5701 cgaagaagcg cgtgacgccc gggtccagcc ccagcgtgcc catgcgcagc gacgagaaca
     5761 tcccctggtc gtagccggga ttggtgatca cccgagcgcc cgcgccggcc gcggccgcgg
     5821 cgacctcgtc gcggcgatgc cccgccacga cgacgacgtc gcggacgccc gcctgcgtga
     5881 acgcgcccac agcccgctcg agcagcgtgc gcccccggac ctcgagcagc ggcttgaagg
     5941 cgcccatgcg ggacgagctg cccgcggcga ggacgatggc ggcgacccgc tccacggctc
     6001 aggcgccggc gccggcgcgc gcctggacga gctcggcggc gatgctcacg gcgagctcgg
     6061 ccggcgtctc cgcgccgatg gcgacgccga tcggcgagtg gacccggccg atggtcccct
     6121 cgtcgaaccc ggcggcgcgc agcgccgcga gcatgcgcgc gcgcttggcg cggctgccca
     6181 tcacgccgac gtagcgggcg ccggcgcgga gcgcctgcac gagcacgtcc aggtcgtgca
     6241 cgtgcccgcg cgtgacgatc accacgtagg cgcgctcgtc cacgcccacc gccgcgaggg
     6301 cgccgtcgaa cggccggacc acgacctcgg cgccggggaa ccgctcggcc gaggcgagat
     6361 cctcgcggtc gtcgaggacg accacgcgga agcccaggcc ggccaggagc ggcgcgaccg
     6421 cgcacccgac gtggccggcg ccacagacga cggcgagtgc cggcggatcc acgcgctcga
     6481 ggacgacgtc gcggccgtcc ggcagggtcg cggtaccgtg aagcgccgcg gcggccgtga
     6541 gacggcgcag cgccgccgcc tcgcagaccg cggcgccggc gagctcgccg ctctccgcca
     6601 gcaggcagtg ctcgacgggg cgggcgaacg agccggcgtc gtcgccgggg ggcgacgcgg
     6661 tcacgtacca ggagcggcgc cccgcgtcgc gcgcatcctt cagggcgcgg cacaccgcga
     6721 gaagctccgc gtcccgcggc ggaacgaagg tgatcagcac gtccgccgcg ccgccgcaga
     6781 ccatcttctc gccgctcgcg aggtcctcgc cgcggaggtc cgcgcgctct cgccgtgaac
     6841 gccgctcggc gagcgccgtc gcggcggcgg tcatcatcgt gtgctcgatc agtccgccgc
     6901 cgatgctgcc ggcgatggag ccgtcgtcgc gcaccagcat ctccgcgccg gcggcgcgcg
     6961 gcgtggagcc ctggtcggcg accagggtga ccagggcgaa cgcgcgcccg gcctccagct
     7021 gctcca
//
LOCUS       DASUMX010000262        25884 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_381867, whole genome shotgun sequence.
ACCESSION   DASUMX010000262 DASUMX010000000
VERSION     DASUMX010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 25884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25884
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381867"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="VFZ86_12380"
     CDS             <1..399
                     /locus_tag="VFZ86_12380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643114.1"
                     /translation="DDIVAVSFMWTMGWYRFHVDIDSGDVTMHDRGYEELTLQPNAGV
                     RADGTVQLAPAQISRAAAQRAQAAPTQATPEPAQPAAQPLAPPAEPETPREAAQKPPE
                     FLSKSLLGQRSDDETASWEQTTARDFDWDR"
     gene            complement(519..1511)
                     /locus_tag="VFZ86_12385"
     CDS             complement(519..1511)
                     /locus_tag="VFZ86_12385"
                     /inference="COORDINATES: protein motif:HMM:NF042666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6612 family protein"
                     /protein_id="HEX5643115.1"
                     /translation="MISRRGAILLLVLALALVVVACGGDEATTADPAAADTASGPPAD
                     QIVKDSEAKMATVNSAAFIADVALKIEGDTAKMTDPTAKALLSDGVTLHAEGSSGNDP
                     TTVNMTMSLGIAGQNLEFGMMSEGEKSWIEYQGTWYALDTKNSKALDEQAAQGAAPTE
                     QLKSMGIDPASWGTEFELVGTEDLNGVEVYHVKAAADPQKLADSLLKAADDPTLGDKL
                     GGSGGELGELSEGLAQNKQQVEELGKTLRAATVDYWIGVDDQYMYKAQFGASMDTSGQ
                     KDMEGVSAVSAQGTVSMSAFDEPVAVEAPENAKSFDTFMNQLFGGMMGGSGMTL"
     gene            complement(1615..2367)
                     /gene="jag"
                     /locus_tag="VFZ86_12390"
     CDS             complement(1615..2367)
                     /gene="jag"
                     /locus_tag="VFZ86_12390"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HEX5643116.1"
                     /translation="MEEPDDLQPIDEEDEEDEEDQEDEETEFATIAGGASIEEAKRTA
                     LEQLRKIVPYVNPADVEYIVVDEGSKGGFFGRGKMEARVEARLRPSDERVEADLPEGA
                     ETLREFIQTVVDLMGVEAHVGASETPERVRAEISGDDLGLLIGRHGATIDALQYIAAI
                     VVNGDRRERRQVIVDAEGYRDRRAAALSALADRTAQKVVRDSVSIALKPMTAAERKVV
                     HLHLKDHPRVETASEGNEPFRAVVVSPKQRTS"
     gene            complement(2417..3532)
                     /gene="yidC"
                     /locus_tag="VFZ86_12395"
     CDS             complement(2417..3532)
                     /gene="yidC"
                     /locus_tag="VFZ86_12395"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEX5643117.1"
                     /translation="MYNFPILSELILLLHWVLVTINDFLEGLGLGYSWTWGLAIIGLT
                     IIVRLILFPLTWKQYKSAQQMQVIQPHVKELQKKYKNDRGKLQEETMKLYSEHRVNPF
                     ASCLPLILQLPVFIALYAAIKGLGPLSAPEYQASVQALNQAPFLWIPQLGFPDPYYIL
                     LVLYVATQMISTELMVATQSDQTQKWIMRAMPIVFVLFLFNFPAGLFVYWVTTNVWTI
                     GQQLVIRKTMPKPEELAARAQAKPKKRSRFMEAMMASQGEAMKQREQKMAAKKGGQDG
                     GAAKQGSVAAGKKPAQGAKKAPPGKGKRKPGQTQGGRPAQGGGQKPKGGSAGQQGKGK
                     PAPRQDTPKPADAAPAVDAGSGGDAGATTSADGPAEE"
     gene            complement(3525..3824)
                     /gene="yidD"
                     /locus_tag="VFZ86_12400"
     CDS             complement(3525..3824)
                     /gene="yidD"
                     /locus_tag="VFZ86_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014137017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEX5643118.1"
                     /translation="MSRILGAVNAGINAVFIGLIRVYQYTLSPMLGQRCKYYPSCSNS
                     AIGALREHGPIKGFGLATWRILRCNPFSNGGYDPVPPRHHHCGHDHTHENVSARV"
     gene            complement(3821..4204)
                     /gene="rnpA"
                     /locus_tag="VFZ86_12405"
     CDS             complement(3821..4204)
                     /gene="rnpA"
                     /locus_tag="VFZ86_12405"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEX5643119.1"
                     /translation="MAAKRSRLSRSSDFQRIYRQGSSTASRFLVLYWFRRPAEPGDEG
                     PRLGLSVSKKLGGAVVRNRVKRLLREAFLASAGQLAEEYDLVVIARPQLIELVTREGE
                     GEKGLVGAAVRELLGRAGLLTAEQA"
     gene            complement(4217..4351)
                     /gene="rpmH"
                     /locus_tag="VFZ86_12410"
     CDS             complement(4217..4351)
                     /gene="rpmH"
                     /locus_tag="VFZ86_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004815746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEX5643120.1"
                     /translation="MKRTYQPNTRKRSKTHGFRKRMSTRAGRSVIKSRRQKGRKRLSA
                     "
     gene            4520..5251
                     /locus_tag="VFZ86_12415"
     CDS             4520..5251
                     /locus_tag="VFZ86_12415"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HEX5643121.1"
                     /translation="MLLEGSIHRFSLAGVLQFLAQGAATGVLEVRDFEEYGFIYLVRG
                     RVEAISLPITDEKLGTRLVKAGCLTEQQLAEALMEDAGLSHDQRKFKPLGQRLIEKGY
                     TTSEEIRHIMERQTLDQVFELAHWTNGVFLYDEPEQMPRFQVAIEGDVQGLLLDAYRR
                     IDEGELARKTRNVVENEVCYACPLGEECGNVIKTKYLKKDVCLWREMAAVLDESTERL
                     RDARQLYRSREADTKQTLDASLDSE"
     gene            5281..6219
                     /locus_tag="VFZ86_12420"
     CDS             5281..6219
                     /locus_tag="VFZ86_12420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HEX5643122.1"
                     /translation="MAGEQGIDVAFRRILDAISDGVYVLGPDHTIVYWSAGAERITGY
                     APDEVVGRRCDEDILVHTDLNGTRLCTGGCPLHDCVDTGREQSIREVFLRRKSGERLA
                     VYVKTTAFEVEGRRYGVEVFGELESVAGKELAGRIQELSDSAVSDPLTGLFNRRYLDA
                     ALAQQFAMFKRLGSRYGVIQMDIDTLKEINDRLGHAAGDEAIRFVAGIVSDHARDMDV
                     AARSGGDEFVIICGLATGDDLAAYGRRLVRLVRDSRFAPAADVALRLTISAGGALVSA
                     ADADERAALARADAAMYDAKHLGRDAFAVAADGSRR"
     gene            6216..7139
                     /locus_tag="VFZ86_12425"
     CDS             6216..7139
                     /locus_tag="VFZ86_12425"
                     /inference="COORDINATES: protein motif:HMM:NF013269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643123.1"
                     /translation="MTPGGGELRRNPASGRLSIVAPARASRPAGAAGPGAGASPCPFC
                     AGNERLTPPEVDAVRPGGGAPDGPGWRVRVVPNKYPALDGRHEVIVHAPAHEAGLEDL
                     GDDALAEVLAVWQRRVTAHLESGAAAVTLIGNLGAGSGASLEHPHEQLFATPVVPPLL
                     LDELVEAERHRNRYGTCVVCDEIERAGDRAVLDGAVAAWVPGACRFNGELWLAPAAHE
                     ADFRVADPAAVAPALRRALTAVKASIAGAPLNFWLHTAPADARGPFHWHLEFAPRTSA
                     LAGFELGTDIALVIKSPVEAAREYRAALPPA"
     gene            complement(7180..8205)
                     /gene="rlmN"
                     /locus_tag="VFZ86_12430"
     CDS             complement(7180..8205)
                     /gene="rlmN"
                     /locus_tag="VFZ86_12430"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEX5643124.1"
                     /translation="MIDQDTIAATLAELGQPAYRARQVYEALTRGLVTSWDAVTTLPL
                     PLRRELAARLSLLTLREVETRAAPKGTARKTLFATADGHPVEAVLMLVAGRATVCVST
                     QVGCAVGCAFCASGRLGLRRDLTAEEVVDQVLHFARILRDEGGRHVTNVVFMGMGEPF
                     HTYDQTLRACRLLNDPAGFGLAARSISVSTVGVVPGIDRFAREPLQLNLAVSLHAATD
                     ELRDRLVPLNRTYPLDAVFAACDRYVKATRRKLLFEYVVLRGVNDTPAQAVALAQRLR
                     HPLYHLNLIAYNETGGAFSRPAPGELEALRLRLERAGVSVTVRHSPGGDIEAACGQLA
                     LRHDAGC"
     gene            8294..9148
                     /locus_tag="VFZ86_12435"
     CDS             8294..9148
                     /locus_tag="VFZ86_12435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643125.1"
                     /translation="MTTVQIIGIAVASLAVLLLIIALVVTRRHDDEPEAEARASSFLD
                     EAPQDTFSGLGKAEHPVEDITLDPALERAAAARSEAAARQAAAAAQAAAEQVQVTGEQ
                     VQRDRQPQPGGLGLDWGPDLSVRATGFDAGPPGPRAESDDETTGELRPDDAAAVPAQH
                     APPAPEGKSGGQVAATQPARPAEGGSAAGEVAAAESGSGGHLVPLSDIIVTTSSKKVD
                     IEDPDVRRMLTELVTFEIDQAAEFRRQGQSIDAILQLTEAEKVSRALGMTESARRIRA
                     MMEELRGE"
     gene            complement(9167..10531)
                     /locus_tag="VFZ86_12440"
     CDS             complement(9167..10531)
                     /locus_tag="VFZ86_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005813663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HEX5643126.1"
                     /translation="MVFITAQEILETVSMIRSENLDVRAVTMGISLDDCAAGEPHRTA
                     ERIHAKIVRRAGKLVEVAAELQEKYGVPIVNRRVSVTPIADVVGSDDPTAYVEVAHAL
                     DAAADEVGIDFLGGFSALVQKGFTRSDTALIEAIPDAVGATQRVCSSVNVASTRAGIN
                     MDAVLRMADVLLAVSKRTAEHDSIGCAKIVVFCNAPEDNPFMAGAMHGSGEAECSINV
                     AISGPGVVRNVVAANPDASFIELADLIKRTTFKITRVGELIAQEASQMLGAELGIIDL
                     SLAPTPAVGDSIAEILECMGLEAVGAPGTTAALAMLNDAVKKGGTMATSSTGGLSGAF
                     IPVSEDKAMARAAADGHLPIEKLEAMTAVCSLGLDMIVVPGDISRDALAGTIADVMAI
                     GMINSKTTGARLIPAAGKQVGDWVTLGGLFGEGPVMKVSTAGNSAFVRRGGRIPAPLQ
                     SLTN"
     gene            complement(10543..10818)
                     /locus_tag="VFZ86_12445"
     CDS             complement(10543..10818)
                     /locus_tag="VFZ86_12445"
                     /inference="COORDINATES: protein motif:HMM:NF001220.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX5643127.1"
                     /translation="MRTTVIVTALGPDGHGLLAQVATTIAEQDANVDDVSQTVANGMF
                     TMIMFVSFDQGETTIAGLKEALEATGDRIGLQISVQHENIFKFMHRI"
     gene            10882..11454
                     /locus_tag="VFZ86_12450"
     CDS             10882..11454
                     /locus_tag="VFZ86_12450"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX5643128.1"
                     /translation="MPPEPTTTEAAPPPPGRLDSLDRTLSRRAAVDWPHPRWFTVPLG
                     GLSLSANYGVLWYVLCLVPLLLGAEDPLLKALYVAAPVTLVEMTGFVIKHFVGRARPP
                     VADPTQAEQIPLPLSKSFPSSHASMAVVGTFTLGALFPQWIPALVALTLVLCFSRVYL
                     GVHYLGDVLGGLLYGLVWAAAWVLLVPAPV"
     gene            11456..12469
                     /locus_tag="VFZ86_12455"
     CDS             11456..12469
                     /locus_tag="VFZ86_12455"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX5643129.1"
                     /translation="MSALGTRGGQLAVATAAVLVLVIGLAAAGAFNGCGSTAAEVGRP
                     SAAVSSSPHGGVAYASAKALARSVGKRPANTWGEIYAREFITGAFQQYGYFPLLDEFI
                     ATAGGRRIHSANIIALKEGETAQRLVVGAHYDSVPVGEGYTDNATGVGLLLEVAARIK
                     NVPTPYTVVFVAFGAEENGSLGAEDYLRGLSDVERGAVIGMIDLDAPAGGDELSVASR
                     FGGATWLRDDALSAADALGVPLVTSPKAPGRPAGVVWAPADDVPFADADIATAILSAV
                     SWEASKGRKLAQTASGPPIWHTPRDTVKYVDTTYPRRVRQQLRQMSLLLETLLTSKLE
                     RTP"
     gene            12466..13566
                     /gene="ychF"
                     /locus_tag="VFZ86_12460"
     CDS             12466..13566
                     /gene="ychF"
                     /locus_tag="VFZ86_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006692058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEX5643130.1"
                     /translation="MKLGIVGLPNVGKTTLFNALTHAQAAATAYAFTSAESNLGVVAV
                     PDPRLDRLDEVLETPRKVNATIDVVDIAGLAEGASRGEGLGNRFLADIRGVDAVAHVI
                     RVFENPEVAHVSDTLDPRADVDLVETELVLSDAELVERRLDKTRKAARSGDKAAKHEL
                     EVLEALHAALMDGNLAKKIERSADDEPLFRELALVSDRPVLFVLNTDDDQSAAAALEQ
                     HAEFVEWARDRGDQVVAVAARLEAELADLEPEDAAEFRAELGAGEAGTLDVVLRAAYE
                     VLGYITFFTGDFRSSESRAWQLPRGWTAKQAAGRIHSDIEHGFVRAQVVNIDDLIELK
                     SFHAAREKALLRTEGKEYVVQDGDVINFMHTG"
     gene            complement(13606..14019)
                     /locus_tag="VFZ86_12465"
     CDS             complement(13606..14019)
                     /locus_tag="VFZ86_12465"
                     /inference="COORDINATES: protein motif:HMM:NF022953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HEX5643131.1"
                     /translation="MRHTTRALVALAALAVLGAAVVGGCGGATDAQPASPSPSAPPPG
                     PADITGVVGDLTPGADAQSVSILVVADGSADDDYDKASVTVGPGATVWTVDGRRGDAS
                     DLAQGARIAVWFEGPVRESYPVQATAGVVQILTSD"
     gene            14132..15109
                     /locus_tag="VFZ86_12470"
     CDS             14132..15109
                     /locus_tag="VFZ86_12470"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5643132.1"
                     /translation="MSSSSRHPSPPPGYRLRATSPDDAAAVARLKAAVDIERHGVSDV
                     TVEYVLEEWALPRLDLARDSWVVEDETGAVVGYGLCWMEAPPDQLAAVQVADPGHRGR
                     GLGALLLRVCEARAEEMVSAAAGHAAVATLGVTAHESDVRRLELLERRGYRRERTFFR
                     LERDLDDSLEPPVWPAAIRTAPFRPGVDDAAVHAAHEEGFADHYGPAEPDLEEWVRSH
                     FADGDPDLGLWLVAWDGAEVVGGIEAMETPSGGFMGELFVRPPWRGRGIGRALMLQEC
                     AELRRRGMQSAFFAVDAANPTGALRLHESIGFRSSHGATHLLEKKLRAP"
     gene            15175..15336
                     /locus_tag="VFZ86_12475"
     CDS             15175..15336
                     /locus_tag="VFZ86_12475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643133.1"
                     /translation="MRERFVVETGGRHAAGRLHGAQLAATRSLFGHARSGRFFGGLWF
                     KIISERGPC"
     gene            15380..16837
                     /locus_tag="VFZ86_12480"
                     /pseudo
     CDS             15380..16837
                     /locus_tag="VFZ86_12480"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
     gene            16932..17828
                     /locus_tag="VFZ86_12485"
     CDS             16932..17828
                     /locus_tag="VFZ86_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010645174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5643134.1"
                     /translation="MSVIKAEHLTKRFGKKVLAVDDVSFAIEAGTITGVLGPNGAGKT
                     TTLRMILDLVRPTSGSVTILGSRYRELRAPAARVGALLDASGFHPGRSGRNALRVIAT
                     GAGIPDARVEESLELVELEDAAKRAVKGYSTGMKQRLALAASLLGDPEVLILDEPANG
                     LDPEGMLWLRTFLRKLAAEGRTVLVSSHVLAEVEQTVDDILIMSKGRLLAQGPIHELT
                     ASTQPVVRVRSPQADRLEVALKSAGAAVNRLDDALAIRGPSAAEVGDLAHARGVALHE
                     LLTEQPSLEELFLQIAGTRREQ"
     gene            17825..18574
                     /locus_tag="VFZ86_12490"
     CDS             17825..18574
                     /locus_tag="VFZ86_12490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643135.1"
                     /translation="MIALARAELLKLRSTRTALGLFIVILVVTVVPLVGLLIFLSPAD
                     LASGGLGAIAVSLVPLVLLVFGILGMTNEYRHGTITYTYLVTPRRWQVMVTKLVAYAV
                     VGIITMLATVVLVYVAIRLGGAIRGVEIDAMGEATLGDYTRQIVVAGLITTFGVALGA
                     LLRAQVVTVAAALIWALFVENIIVVLKPAVGAWLPFTVFQQVGAGQTVDAGTPAASEM
                     LTRPAAFIVSLVYIAVVSVAAVFISLRRDVT"
     gene            18583..19080
                     /locus_tag="VFZ86_12495"
     CDS             18583..19080
                     /locus_tag="VFZ86_12495"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5643136.1"
                     /translation="MEHFSIRGPMLGQGEAAERILRALPGWFGIESALLDYVRAADEL
                     PTLVALPPGTGRAGREVRADEVIGFVTLKETSEDALELHVMGVLPAWRRRGAGRALVE
                     HAAAYSRAEGFSLLHVKTLAPSDPDPGYAATRAFYAAAGFRPLEELPQVWGPENPCLL
                     MVRPL"
     gene            complement(19077..19610)
                     /locus_tag="VFZ86_12500"
     CDS             complement(19077..19610)
                     /locus_tag="VFZ86_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009108009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HEX5643137.1"
                     /translation="MLDQKMQDAFNSQINWELYSGYLYLSMASKFADLGMSGGQNWMT
                     VQYQEELAHARIMFSYVLTRGGRVILEAIDRPESEWPGGLEMYQEALEHEQGVTARIH
                     DMASLALELKDHATYNFLQWFIAEQVEEEETAMDWVGKFKMVGEHPAGLYQLDKELAA
                     RTYTAPTPLAALDAAGA"
     gene            19747..20241
                     /locus_tag="VFZ86_12505"
     CDS             19747..20241
                     /locus_tag="VFZ86_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HEX5643138.1"
                     /translation="MNRAFVKEPDEGAPAEGLPERQISEHPNYVTPAGLRQLEARVGE
                     LETRRLELLEEDDLEGELAYVDRDLRYYTTRLESAILVDPDRQPHRKVAFGAAVTVSQ
                     NGGDLRTFTIVGEDEADLKEGKISFVSPLAEALLEARVGKTVLWRRPAGDLKLTVEEI
                     DYPA"
     gene            complement(20281..21528)
                     /locus_tag="VFZ86_12510"
     CDS             complement(20281..21528)
                     /locus_tag="VFZ86_12510"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX5643139.1"
                     /translation="MSERRTIYESARGRRRRRLRAAKLAALVAVLALAAAAVAAAFAL
                     RDGSGSADGAAASAAPSSAPSPSPARLARLADVEVRQGDKATFRYRVVAAAGTTAVTL
                     VVRDAGGQQVKARRLTEVAEPGERLEAVVPIDLPPGRYSYELRLGDEGLASPSPSTAQ
                     ASDGVASATLVVRKPLPPGFPGENAVAAASEWISGRDGDAAFAVVDTNGEDAGGHRQH
                     ERFQLASLSKAILLVASLRDDPTPDPATEATLTKMITESDNGAANTVFGQVGAKGMRA
                     VAKAAGLEDYEQGSGWIDTRDSARDQARFFWQLESLVPPKGRALARELLAGVIPIQRW
                     GIPAAAGPEGWTSFFKGGWLGLDNKLMTQAAWLENGKKRWALAVMGDENPTRSYGWDT
                     QKGVTGLLLGQEPTPAYLSVVLE"
     gene            complement(21648..21745)
                     /locus_tag="VFZ86_12515"
     tRNA            complement(21648..21745)
                     /locus_tag="VFZ86_12515"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21709..21711),aa:Sec,seq:tca)
     gene            complement(22252..23550)
                     /locus_tag="VFZ86_12520"
     CDS             complement(22252..23550)
                     /locus_tag="VFZ86_12520"
                     /inference="COORDINATES: protein motif:HMM:NF027964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HEX5643140.1"
                     /translation="MKALVVGTGGVGQSIASIAKQRDPEGAWLEKMVMADYDGKRAAD
                     FVATLADPRFVAEQVDASDADAVAALAAKHGADILMNLCAPNFNPPLLQAALKAKVHY
                     LDTAMTLSEWHPTDPFNKTGKKLGDDEYALSPQFEAIGKYALAGFGVEPGMADFFARY
                     AADHLFDEVDYIGIRDGSNLEIPGAEGISFGFSVWTTIEECNNPAIVFENGDFHTVRP
                     FAELEKFYLPEGIGEVEVGHVEHEEVVQIGRNADRLKGVKKAEFKYALGDEFMDFMSA
                     LRSINMDKAEKIKVGNVEVAPRDVIAAAAPDPNEIGKKYVGKTAAGTYVVGKKDGMER
                     KVYLYQVADNQECLERVGVQVVTAQTGFTPVITLELLATGSLGSKPGDPKSGVHNPEA
                     FNADDYVALMPKVGFPGGLLEMESEYKLAHDRQALLDLAK"
     gene            complement(23713..24906)
                     /locus_tag="VFZ86_12525"
     CDS             complement(23713..24906)
                     /locus_tag="VFZ86_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-NAD kinase family protein"
                     /protein_id="HEX5643141.1"
                     /translation="MKKLGLIVNPVAGVGGRVGLKGSDGADVLRRALELGAVRDAPRR
                     ARQALERLGRVREHVEVLAWPGEMGEDEARAAGWEPRVLGSFDGRRSYVLCELERSGA
                     RPAIACDDFVLTTPADTEQAARDLLAAGVDLILFAGGDGTARNICNAVGDAVPVIGVP
                     AGVKIHSAVYATTPAAAGDVAALYLYERPAAVQLREGEVMDIDEEAFRENRVSAHLYG
                     YMTVPYARGLTQSAKAGGVAGEERALNDIATEVIAGMEPGALYILGPGTTTRTVMERL
                     GLPKTLLGVDAVRDGELAGVDLTEGELLALIAAAGQASIVVTVIGGQGHIFGRGNQQI
                     SPAVIRAAGTDHIVVIATQTKLLSLEGRPLLVDTGDPALDAELAGYAKVVTALGERTM
                     YRVGA"
     gene            complement(24934..25428)
                     /locus_tag="VFZ86_12530"
     CDS             complement(24934..25428)
                     /locus_tag="VFZ86_12530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643142.1"
                     /translation="MPQQPAKPGIRVLEAFGLTVFLATAAFLTALFLSGLGGVFESLE
                     TAAAAFSTLCATVAAFAMLIDAADLWVRGRKMSPYAVKMFRSLVFIAVLGALAASLLG
                     GNSLVIPLLVPAMIVYLFIARKRPAARYATAPGGARGGSGGGRTASAAPTKSRQRKGG
                     KKHR"
BASE COUNT         3621 a         9241 c         9246 g         3776 t
ORIGIN      
        1 gacgacatcg tcgccgtcag cttcatgtgg acgatgggct ggtaccggtt ccacgtcgac
       61 atcgacagcg gcgacgtgac catgcacgac cgcgggtacg aggagctcac gctgcagcct
      121 aacgccggcg tgcgtgccga cggcacggtg cagctcgctc cggcgcagat cagccgcgcc
      181 gccgcccagc gcgcccaggc ggcgcccacc caggcgacgc cggagccggc ccagcccgcg
      241 gctcagccgc tcgcccctcc ggccgagccg gagacgccgc gcgaggccgc gcagaagccg
      301 cctgagttcc tcagcaagtc gctgctgggc cagcgcagcg acgacgagac ggcgtcgtgg
      361 gagcagacca cggcgcgcga cttcgactgg gaccgctgag ccgctggcgc gacgtggcct
      421 gcgcgcgccg ccgtacgtga ggagagccgg ggccgcctcg cggcgccccg gctctcctcg
      481 tcttcgggcg gcgctcgtcg cgtcgcgccg gcgtcacctc agagcgtcat cccgctgccg
      541 cccatcatcc cgccgaacag ctggttcatg aacgtgtcga acgacttcgc gttctccggc
      601 gcctcgaccg cgacaggctc gtcgaaggcg ctcatgctga cggtgccctg cgcgctcacc
      661 gcggagacgc cctccatgtc cttctggccc gaggtgtcca tggacgcccc gaactgcgcc
      721 ttgtacatgt actggtcgtc cacgccgatc cagtagtcga ccgtcgcggc ccggagcgtc
      781 ttgcccagct cctcgacctg ctgcttgttc tgcgccagac cctcgctcaa ctcaccgagc
      841 tcgccaccgg aaccgccgag cttgtccccg agcgtcgggt cgtcggcggc cttgaggagc
      901 gagtccgcca gtttctgcgg gtcggcggcg gccttgacgt gatagacctc gacgccgttc
      961 aggtcctcgg tgccgacgag ctcgaactcg gtaccccagc tcgccggatc gatgcccatg
     1021 ctcttgagct gctctgtcgg ggcagcccct tgcgcggcct gctcgtcgag cgccttcgag
     1081 ttcttggtgt ccagcgcgta ccaggtgccc tggtactcga tccacgactt ctcgccctcg
     1141 ctcatcatgc cgaactcgag gttctggccg gcgatcccga ggctcatcgt catgttcacg
     1201 gtggtcgggt cgttgccgct cgagccctcg gcgtggaggg tgacgccgtc cgacagcagc
     1261 gccttcgccg tcgggtcggt catcttggcg gtgtcgcctt cgatcttcag ggcgacgtcg
     1321 gcgatgaacg cggcgctgtt caccgtcgcc atcttggcct cgctgtcctt gacgatctgg
     1381 tcggccggcg ggccggaagc cgtgtcggcg gcggcgggat cggcggtggt ggcctcgtcg
     1441 ccgccgcagg ccacgacgac gagcgccagc gccagcacga ggagcaggat cgccccgcgt
     1501 ctggaaatca tggcacgcat aggtgtcctc ctgtcgtcat ccgggcgcga tcggcgcccg
     1561 ctcctcttct cggccgggag cgctcgctcc tttacggagt gatgcccgcg cggctcagct
     1621 tgtgcgctgt ttgggagaga cgaccacagc ccggaacggt tcgttgccct cggacgcggt
     1681 ctccacgcgc gggtggtcct tgaggtgcag gtggaccacc ttgcgctccg ccgccgtcat
     1741 cggcttgagg gcgatgctca cggagtcgcg cacgaccttc tgcgccgtcc ggtcggcgag
     1801 cgccgagaga gccgcggcgc ggcggtcgcg gtagccctcc gcgtcgacga tcacctggcg
     1861 acgctcgcgg cggtcgccgt tgacgacgat cgcggcgatg tactgaaggg cgtcgatggt
     1921 ggcgccgtgg cgcccgatga ggagccccag gtcgtcgccg ctgatctccg cgcgtacccg
     1981 ttcgggcgtc tcactggcgc ccacgtgcgc ctcgacgccc atcaggtcga cgacggtctg
     2041 gatgaactcg cggagtgtct cggcgccctc gggcaggtcg gcctcgacgc gttcgtccga
     2101 cggccgcaga cgcgcctcga cgcgagcctc catcttgccg cgaccgaaga agccgccctt
     2161 gctgccctcg tcgacgacga tgtactcgac gtcggccggg ttcacgtagg ggacgatctt
     2221 gcgcagctgc tccagcgccg tgcgcttagc ctcttcgatg gaggccccgc cggcgatggt
     2281 ggcgaactcg gtctcttcgt cctcctggtc ctcttcgtcc tcttcgtcct cttcgtcgat
     2341 cggctggagg tcgtcgggct cttccatcga tcctgctccc tggtcgggtg agaagagcgc
     2401 gtgcgccccg gcggcgtcac tcctcggccg ggccgtccgc gctcgtggtc gctcctgcgt
     2461 cgccgcccga gccagcgtcg acggccggcg ccgcgtctgc gggcttgggc gtgtcctgcc
     2521 ggggcgccgg cttgcccttg ccctgctggc cggcggaacc gcccttgggc ttctggccgc
     2581 cgccctgggc gggcctgccg ccctgcgtct gacccggctt gcgcttgccc ttgccgggcg
     2641 gcgccttctt ggcgccctgc gcgggcttct tgcccgccgc gacgctgccc tgcttggcgg
     2701 cgccgccgtc ctggccgccc ttcttggcgg ccatcttctg ctcgcgctgc ttcatggcct
     2761 caccctgcga cgccatcatc gcctccatga agcgactgcg cttcttgggc ttggcctggg
     2821 cgcgggcggc cagctcctcg ggcttgggca tggtcttgcg gatgacgagc tgctggccga
     2881 tggtccacac gttcgtcgtg acccagtaca cgaacaggcc ggccgggaag ttgaacagga
     2941 agagcacgaa gacgatcggc atcgcgcgca tgatccactt ctgcgtctgg tcgctctgcg
     3001 tcgccaccat gagctcggtc gagatcatct gcgtggccac gtacaggacc agaaggatgt
     3061 agtacggatc ggggaaccca agctgtggga tccacaggaa cggcgcctgg ttgagcgcct
     3121 gcaccgaggc ctgatactcg ggagcgctga gcggcccgag tcccttgatg gccgcgtaca
     3181 gcgcgatgaa caccggcagc tgcaggatga gcgggaggca cgaggcgaac gggttgaccc
     3241 ggtgctccga gtagagcttc atcgtctctt cctggagctt gccgcggtcg ttcttgtact
     3301 tcttctgcag ttccttgacg tgcggctgga tgacctgcat ctgctgcgcc gacttgtact
     3361 gcttccaggt gagcgggaag aggatcagcc gcacgatgat ggtgaggccg atgatggcca
     3421 ggccccaggt ccagctgtag cccagcccga ggccttccag gaagtcgttg atcgtgacga
     3481 ggacccagtg cagcagcagg atgagctcgg agaggatcgg gaagttatac acgcgcgctc
     3541 acattctcgt gggtgtggtc gtgcccgcag tggtggtgac gcggcggcac ggggtcgtag
     3601 ccgccgttgc tgaacgggtt gcagcgcagg atgcgccacg tcgcgaggcc gaagcccttg
     3661 atgggaccgt gctcgcgcag cgcgccgatg gcggagttgg agcacgacgg gtagtacttg
     3721 cagcgctgcc cgagcatggg cgagagggtg tactggtaca ccctgatgag gccgatgaag
     3781 accgcgttga tcccggcgtt gacggcgccc aggatgcggc tcatgcctgc tccgcggtga
     3841 ggagcccggc gcggcccagg agctcgcgga cggccgcgcc cacgaggccc ttctcgccct
     3901 cgccctcgcg ggtgacgagc tcgatcagct gcgggcgcgc gatcacgacc aggtcgtact
     3961 cctcggcgag ctgccccgcg ctggccagga aggcctcgcg cagcagccgt ttgacacggt
     4021 tacggacgac ggcgccgccc agcttcttcg acaccgagag gccgagtcgc ggcccctcgt
     4081 cgcccggctc ggccgggcgc ctgaaccagt acagcacgag gaagcgagaa gccgtcgagc
     4141 tcccttgccg gtagatgcgc tggaagtcgg aggagcgaga aaggcgagag cgcttggcgg
     4201 ccatcggggc ccgcggctac gcggagaggc gcttgcgccc cttctgccgg cggctcttga
     4261 tcaccgaccg acccgcgcgc gtgctcatgc gcttgcgaaa gccatgggtc ttgctgcgct
     4321 tgcgggtgtt gggctggtag gtcctcttca ctgcttcctc gcacgattgc cgacagtcag
     4381 aacgtggagt atagccgcgc ggcggaggcc cggtcaattt ggcccgcagg gcccggcccg
     4441 ggcctccgca gcgctcacga atccccagcc actgccgata ggggctaccg agaggaaagt
     4501 cctgctcagg caaggccgta tgctgctcga aggatccatc cacaggttca gcctcgccgg
     4561 cgtcctgcag ttcctcgcgc agggcgccgc caccggcgtg ctcgaggtcc gcgacttcga
     4621 ggagtacggc ttcatctatc tggtgcgcgg ccgcgtcgag gcgatctcgc tgccgatcac
     4681 cgacgagaag ctcggcacgc ggctggtcaa ggctggctgt ctcaccgagc agcagctggc
     4741 cgaggccctc atggaggatg cgggcctctc ccacgaccag aggaagttca agccgctcgg
     4801 ccagcgcctc atcgagaagg gctacaccac gagcgaggag atccgccaca tcatggagcg
     4861 gcagaccctc gaccaggtgt tcgagttggc gcactggacc aacggcgtgt tcctctacga
     4921 cgagcccgag cagatgccgc gcttccaggt tgcgatcgag ggcgacgtgc agggcctcct
     4981 gctcgacgcc taccggcgca tcgacgaggg cgagctcgcg cgcaagacgc gcaacgtcgt
     5041 ggagaacgag gtctgctatg cgtgtccgct cggtgaagag tgcgggaacg tgatcaagac
     5101 caagtacctg aagaaggatg tatgcctctg gcgcgagatg gcggccgtgc tcgacgagag
     5161 caccgagcgg ctgcgcgacg cgcgccagct ctaccgttcg cgcgaagcgg acaccaagca
     5221 gacgctcgac gcgtccctcg actcagagtg agccgggcgc gaagagggta ctgactgcgc
     5281 gtggccggtg agcagggaat agacgttgcg ttcaggcgca tcctcgacgc catcagcgac
     5341 ggggtgtacg tcctcggccc cgaccacacc atcgtgtact ggagcgccgg cgccgaacgg
     5401 atcaccggct acgcgccgga cgaggtcgtc ggtcggcgct gcgacgagga catcctcgtc
     5461 cacaccgatc tgaacgggac gcggctgtgc accggcggct gcccgctcca tgactgcgtc
     5521 gacacggggc gcgagcagtc catccgcgag gtgttcctca ggcgcaagag cggcgaacgc
     5581 ctggccgtgt acgtcaagac caccgcgttc gaggtcgagg gtcgccgcta cggtgtcgag
     5641 gtcttcggcg agctcgagag cgtggccggc aaggagctcg ccgggcgcat ccaggagctg
     5701 tccgactccg ccgtgtccga cccgctcacc ggcttgttca accggcgcta cctcgacgcc
     5761 gcgcttgccc agcagttcgc catgttcaag cggctgggaa gcaggtacgg cgtcatccag
     5821 atggacatcg acacgctcaa ggagatcaac gaccggctcg gtcacgccgc gggggacgag
     5881 gccatccgct tcgtggccgg catcgtctcc gaccacgcga gagacatgga cgtggccgcc
     5941 aggtccggcg gcgacgagtt cgtcatcatc tgcgggctcg cgaccgggga cgacctcgcc
     6001 gcgtatggcc ggcgcctggt gcgcctggtc cgcgactcgc gcttcgcgcc ggcggcggac
     6061 gtcgcgctgc gcctgacgat ctcggccggc ggcgccctcg tgagcgcggc cgacgccgac
     6121 gagcgcgccg cgctggcacg cgccgacgcc gccatgtacg acgccaagca cctcgggcgc
     6181 gacgccttcg ccgtcgccgc cgacgggagc cgaaggtgac gcccggcggc ggcgagctgc
     6241 gccgcaatcc cgccagcggc cgcctctcga tcgtcgctcc ggcgagggcg tcccggcccg
     6301 ccggtgcggc cgggccgggc gccggcgcgt ctccctgccc gttctgcgcc ggcaacgagc
     6361 ggctcacgcc gccggaggtc gacgccgtgc ggcccggcgg cggcgcgcca gacggcccgg
     6421 gctggcgcgt gcgcgtggtg cccaacaagt accccgccct cgacgggcgg cacgaggtga
     6481 tcgtgcacgc gcccgcgcac gaggcgggac tggaggacct cggagacgac gcgctcgccg
     6541 aggtcctcgc cgtgtggcag cgcagggtca cggcgcacct cgagtcggga gcggccgcgg
     6601 tgacgctcat cggcaacctc ggcgccggct cgggcgcctc gctggagcac ccgcacgagc
     6661 agctgttcgc gacgccggtg gtgccgccgc tgctgctcga cgagctcgtc gaggccgagc
     6721 ggcaccgcaa ccgctacggg acgtgcgtgg tgtgcgacga gatcgagagg gcgggcgacc
     6781 gggccgtgct ggacggcgcc gtcgctgcct gggtgcccgg cgcctgccgc ttcaacggcg
     6841 agctctggct ggcgccggcg gcccacgaag cggacttccg tgtcgcggat cccgccgccg
     6901 tggccccggc gctgcgccgt gcgctcaccg ccgtcaaggc gtcgatcgcc ggggccccgc
     6961 tgaacttctg gctgcacacc gcgccggccg acgcgcgcgg accgttccac tggcacctcg
     7021 agttcgcacc gcgcacctcg gccctggccg gcttcgagct gggcacggac atcgccctcg
     7081 tcatcaagag cccggtggag gcggcccggg agtaccgcgc cgccctgccg ccggcctgac
     7141 cgaggggccc cctcagcggc ggcggtcgaa ccccgcggat cagcagcccg cgtcgtggcg
     7201 cagcgccagc tgcccgcagg cggcctcgat gtcgccgccg ggcgagtgcc gcacggtcac
     7261 gctcacaccg gcccgctcca gacgcaggcg cagcgcctcg agctcccccg gagccgggcg
     7321 cgagaaggcg ccgccggtct cgttgtaggc gatcaggttg aggtggtaca gggggtggcg
     7381 cagccgctgc gccagcgcca ccgcctgcgc cggcgtgtcg ttgacgccgc gcagcaccac
     7441 gtactcgaag agcagcttgc gccgcgtggc cttcacgtag cggtcgcacg cggcgaacac
     7501 cgcgtccagg gggtacgtgc ggttgagcgg caccagacgg tcgcgcaact cgtcggtcgc
     7561 ggcgtgcagg ctcacggcga gattcagctg cagcggctcc cgggcaaagc ggtcgatgcc
     7621 gggaacgacg ccgacggtcg agacggagat ggaccgcgcg gccagaccga atcccgccgg
     7681 gtcgttgagc agacgacagg cgcgcagcgt ctgatcgtag gtgtggaacg gctcccccat
     7741 ccccatgaag acgacgttgg tgacgtgccg cccgccttcg tcgcggagga tgcgggcgaa
     7801 gtgcaggacc tggtcgacga cctcctcggc cgtcaggtcc cggcggagcc caagcctgcc
     7861 cgaggcgcag aaggcgcagc ccacggcgca gccgacctgg gtcgagacgc agacggtggc
     7921 cctgccggcc accagcatga gcaccgcctc cacggggtgg ccgtccgccg tggcgaacag
     7981 cgtcttgcgc gccgtgccct tcggggcggc gcgcgtctcc acctcgcgca gcgtgagcag
     8041 cgagaggcgc gccgccagtt cccggcgcaa cggcagcggc agcgtcgtga cggcgtccca
     8101 gctcgtgacc aggccgcgcg tgagcgcctc gtagacctgg cgggcacggt aggcgggctg
     8161 gccgagctcg gcgagcgtgg cggcgatggt gtcctgatcg atcacccggc cattctacgc
     8221 cgggcaggga atggtggagg ggaaggcgaa gagggtacgc gccaggcacg agcggacccg
     8281 ggaacccaac gcaatgacca ccgtgcagat catcggcatc gccgtagcgt ccctcgcggt
     8341 gctcctgctc atcatcgcgc tcgtcgtcac gaggcgccac gatgacgagc cggaggcgga
     8401 ggcgcgcgcg tcctcattcc tcgacgaggc cccgcaggac accttctcgg ggctcggcaa
     8461 ggccgagcat ccggtcgagg acatcaccct cgacccagca ctggagcggg cggcagccgc
     8521 ccggagcgag gccgcggcga ggcaggcggc cgcagcagcg caggcggccg ccgagcaggt
     8581 gcaggtgacc ggcgagcagg tgcagcggga ccggcagccc cagccgggtg gcctcgggct
     8641 cgactggggc ccggacctca gcgtacgcgc gacggggttc gacgcgggac cgccgggacc
     8701 ccgagcggaa agcgatgacg agaccacggg agagctgcgg ccggacgacg ccgcagcggt
     8761 cccggcccag cacgctcccc cggcccccga ggggaagagc ggcggccagg tggccgccac
     8821 gcagcccgcg cggcccgctg agggcgggtc cgccgccggc gaggtggcgg cagcagagtc
     8881 cggctccggc ggtcacctgg tcccgctctc cgacatcatc gtcacgacga gcagcaagaa
     8941 ggtcgacatc gaggacccgg acgtgcgacg gatgctgacg gagctggtga cgttcgagat
     9001 cgaccaggcg gcggagttcc gccggcaggg tcagtccatc gacgcgatcc tgcagctcac
     9061 ggaggccgag aaggtctcgc gcgcgctcgg catgaccgag tcggcgcggc ggatccgggc
     9121 catgatggag gagctccgcg gcgagtagcc gcagccgagc gctgctctag ttcgtcagcg
     9181 actggagcgg ggcaggtatg cgccctcccc gccgcacaaa ggcgctgttg ccggcggtgc
     9241 tcaccttcat caccgggcct tcgccgaaca gcccgcccag cgtgacccag tccccgacct
     9301 gcttgccggc cgcggggatg agccgcgccc cggtggtctt ggagttgatc atgccgatgg
     9361 ccatgacgtc ggcgatggtg cccgccagcg cgtcgcggga gatgtcgccg ggaacgacga
     9421 tcatgtccag cccgagcgag cagacggcgg tcatcgcctc gagcttctcg atcggcaggt
     9481 ggccgtcggc ggcggcccgg gccatcgcct tgtcctcgct caccgggatg aacgccccgg
     9541 agaggccgcc cgtggacgac gtcgccatcg tcccgccctt cttcaccgcg tcgttgagca
     9601 tcgccagcgc ggcggtcgtg ccgggagcgc cgaccgcctc gaggcccatg cactcgagga
     9661 tctcggcgat cgagtcgccg accgccggcg tcggcgcgag tgagaggtcg atgatgccca
     9721 gctcggcgcc cagcatctgc gaggcctcct gggcgatcag ctcgccgacg cgggtgatct
     9781 tgaaggtcgt gcgcttgatg aggtcggcga gctcgatgaa cgaggcgtcc gggttggcgg
     9841 cgacgacgtt gcgcaccacg cccggcccgg agatggcgac gttgatgctg cattcggcct
     9901 cgccgctgcc gtgcatcgcg ccggccatga acgggttgtc ctccggcgcg ttgcagaaca
     9961 cgacgatctt ggcgcagccg atggagtcgt gctctgccgt ccgcttgctc accgcgagca
    10021 ggacgtcggc catgcgcagc acggcatcca tgttgatgcc cgcgcgcgtg gaggccacgt
    10081 tgacggagga acacacgcgc tgggtggcgc cgacggcgtc ggggatcgcc tcgatgaggg
    10141 cggtgtcgct gcgcgtgaac cccttctgga ccagcgcgct gaagccgccg aggaagtcga
    10201 tcccgacctc gtcggcggcc gcgtcgagcg cgtgcgccac ctcgacgtag gcggtcgggt
    10261 cgtcgctgcc gaccacatcg gcgatcggcg tcaccgagac gcgccggttg acgatcggca
    10321 cgccgtactt ctcctgcagc tccgcggcga cctcgaccag cttgccggcg cgacgcacga
    10381 tcttggcgtg gatgcgctct gcggtgcgat gcggctcgcc ggcggcgcag tcgtcgagtg
    10441 agatgcccat ggtgacggcc cggacgtcga ggttctcgga ccggatcatg ctcacggtct
    10501 cgaggatctc ctgggcggtg atgaagacca cggccgccgc ccctagatgc ggtgcatgaa
    10561 cttgaagatg ttctcgtgct gcacgctgat ctgcagcccg atgcggtccc ccgtcgcctc
    10621 cagcgcctcc ttgagcccgg cgatggtcgt ctcaccctgg tcgaacgaga cgaacatgat
    10681 catcgtgaac atgccgttgg cgaccgtctg gctgacgtcg tcgacgttgg cgtcctgctc
    10741 ggcgatggtc gtcgcgacct gggccagcag cccgtgcccg tcggggccga gagcggtgac
    10801 gatgacggtt gtgcgcatcg gggacgtccc tgggagaagg tggccgggag caggcgacca
    10861 gtatactagg ccgcgatccc gatgcccccc gaacccacga ccacagaagc cgcgcccccc
    10921 cctcccggcc gcctcgacag cctcgaccgc accctctcgc ggcgggcggc cgtggactgg
    10981 ccgcacccgc gctggttcac cgtaccgctc ggcgggctct cgctctccgc gaactacggg
    11041 gtgctctggt acgtcctctg tctggtgccc ctgcttctcg gcgccgagga ccccttgctg
    11101 aaggcgttgt acgtggccgc accggtcaca ctggtggaga tgaccggatt cgtgatcaag
    11161 cacttcgtcg ggcgcgcacg acctcccgtg gccgacccta cgcaggccga gcagatcccg
    11221 ctgccgctga gcaagtcgtt cccgtcgtcg cacgcgagca tggcagtggt cggcacgttc
    11281 accctgggcg ccctcttccc gcagtggatc ccggcgctgg tcgcgctcac gctggtcctg
    11341 tgcttcagcc gcgtgtacct cggcgtgcac tacctcggcg acgtgctcgg cggcctcctc
    11401 tacgggctcg tctgggccgc cgcctgggtc ctcctcgtgc cggcgccggt gtgacgtgag
    11461 cgccctgggt acgcgcggcg gccagctggc ggtcgccacc gccgccgtcc tcgtcctggt
    11521 catcgggctc gccgcggccg gagcgttcaa cggctgcggg agcacggccg ccgaggtcgg
    11581 gcgcccttcc gcagccgtgt cctcctcgcc ccacggcggc gtcgcctacg cgagcgccaa
    11641 ggcgctcgca cgcagcgtgg gcaagcgccc cgcgaacacc tggggcgaga tctacgctcg
    11701 cgagttcatc accggcgcgt tccagcagta cggctacttc ccgttgctgg acgagttcat
    11761 cgccaccgcc ggcgggcgcc gcatccactc cgccaacatc atcgccctca aggagggcga
    11821 gaccgcccag cggctcgtgg tcggcgccca ctacgattcc gtccccgtgg gcgagggcta
    11881 caccgacaac gcgaccggcg tcggcctgct gctcgaggtc gccgcccgca tcaagaacgt
    11941 gccgacgccg tacacggtcg tgttcgtggc gttcggcgcc gaggagaacg gatccctggg
    12001 cgccgaggac tacctgcgcg ggctcagcga cgtggagcgg ggcgccgtca tcgggatgat
    12061 cgacctggat gcgccggccg gcggcgacga gctctccgtc gccagccgct tcggcggcgc
    12121 gacctggctg cgcgacgacg cgctgagcgc ggccgacgcg ctcggcgtgc cgctggtgac
    12181 gagcccgaag gcgccgggcc ggccggccgg cgtcgtgtgg gcgccggccg acgacgtgcc
    12241 gttcgccgac gccgacatcg cgacggcgat cctgagcgcc gtctcctggg aggcgagcaa
    12301 gggccgcaag ctcgcccaga cggcgagcgg ccctcccatc tggcatactc cgcgggacac
    12361 cgtgaagtac gtcgacacca cgtacccgag gcgcgtgcgg cagcaactgc gacagatgtc
    12421 cctcctcctc gagaccctgc tgaccagcaa actggagagg accccgtgaa gctcggcatc
    12481 gtcggtctgc ccaacgtcgg caagaccacc ctgttcaacg cgctgacgca cgcgcaagcg
    12541 gccgcgaccg cctacgcgtt cacgtcggcg gagtccaacc tcggcgtggt cgcggtgccg
    12601 gacccgcggc tcgaccggct cgacgaggtc ctggagacgc cgaggaaggt caacgccacc
    12661 atcgacgtgg tggacatcgc cgggctggcc gagggcgcga gccgcggcga ggggctgggg
    12721 aaccggttcc tcgccgacat ccgcggcgtc gacgcggtgg cgcacgtgat ccgcgtgttc
    12781 gagaaccccg aggtggcgca cgtgagcgac accctggatc cgcgcgcgga cgtcgacctc
    12841 gtggagacgg agctcgtgct cagcgacgcc gagctcgtgg agcgccgcct cgacaagaca
    12901 cgcaaggcgg cgcgcagcgg cgacaaggcg gccaagcacg agctcgaggt gctcgaggcg
    12961 ctgcacgcgg cgctcatgga cggcaacctg gcgaagaaga tcgagcgctc ggccgacgac
    13021 gagccgctct tccgcgagct cgcgctggtc agcgaccgcc cggtgctgtt cgtcctgaac
    13081 accgacgacg accagagcgc cgcggccgcc ctggagcagc acgcggagtt cgtggagtgg
    13141 gcgcgcgacc gcggcgacca ggtggtcgcc gtcgcggcgc gcctcgaggc cgagctcgcc
    13201 gacctcgagc cggaggacgc ggccgagttc cgcgccgagc tcggcgccgg cgaggccggc
    13261 accctcgacg tggtgctgcg cgccgcctac gaggtgctcg ggtacatcac cttcttcacc
    13321 ggcgacttcc gcagcagcga gtcgcgcgcg tggcagctgc cccgcggctg gaccgccaag
    13381 caggccgcgg gccgcatcca cagcgacatc gagcacgggt tcgtgcgcgc ccaggtggtc
    13441 aacatcgacg acctcatcga gctcaagtcg ttccacgccg cgcgtgagaa ggcgctgctg
    13501 cgcacggagg gcaaggagta cgtcgtgcag gacggcgacg tgatcaactt catgcacacc
    13561 ggctgagcgc cgggcaccgc gcgtgccgac ccggcacgcc gccagtcagt cgctcgtgag
    13621 gatctgcacg accccggccg tggcctgcac cggataggac tccctgaccg gcccctcgaa
    13681 ccacacagcg atccgcgcgc cctgcgcgag atcgctcgca tccccgcgcc gtccatccac
    13741 ggtccagacg gtcgcgccgg gcccgacggt caccgaggcc ttgtcgtagt cgtcgtccgc
    13801 ggacccgtcg gcgaccacga ggatgctcac cgactgcgcg tcggcaccgg gagtgaggtc
    13861 acccacgacg cccgtgatgt ccgcaggtcc gggcggcggc gcggagggcg acggcgaagc
    13921 cggctgggcg tccgtcgcac ccccgcaacc gccgacgacg gctgccccca gcaccgcgag
    13981 tgcggccagg gccaccagcg ctcgagtcgt gtgtctcatg agcgcacctc ccccagcgcc
    14041 gtcggcgacg ccgtcgtctg cacacggaga cgcgccgcgt acaaccggcg ttcgcgtccg
    14101 gctgtagaat cgagggcgtc ttctccctgc cgtgagctct tcctcccgac atccgagccc
    14161 gccccccggg taccgcctga gggcgacatc gcccgacgac gccgcagccg tcgcccggct
    14221 caaggccgcc gtggatatcg agcggcacgg cgtgtcggac gtgaccgtgg agtacgtgct
    14281 cgaggaatgg gcgctgcctc gcctcgacct cgcgcgagat tcgtgggtcg tcgaagacga
    14341 gacgggcgcc gtcgtcggct acgggctctg ctggatggag gcgccgcccg atcagctggc
    14401 ggccgtgcag gtggccgacc ccggccaccg cggacgtgga ctgggcgcac tgctgctccg
    14461 cgtgtgcgaa gcgcgggcgg aagaaatggt cagtgccgcc gccgggcatg cggcggtcgc
    14521 caccctcggg gtcacggcgc acgagagcga cgtgcgccgg ctggagctgc tcgagcggcg
    14581 cggctatcgg agggaacgca ccttcttccg cctcgaacgc gacctcgacg acagcctgga
    14641 gcccccggtg tggccggccg cgatccggac ggcgccgttc cgccccggag tcgacgatgc
    14701 cgcggtccac gccgcccacg aggaggggtt cgccgaccac tacgggcctg ccgagccgga
    14761 cctcgaggag tgggtccggt ctcacttcgc cgacggggat cccgacctcg gtctctggct
    14821 ggtcgcgtgg gacggcgccg aagtggtggg cggcatcgag gccatggaga ccccgtccgg
    14881 gggcttcatg ggggagctct tcgtgcgccc gccgtggcgc ggccgcggca tcggcagggc
    14941 gttgatgctc caggagtgcg ccgagctgcg ccggcgcggg atgcagagcg cgtttttcgc
    15001 cgtcgacgcc gccaacccca cgggggcgct gcgcctgcac gagtccatcg gcttccgctc
    15061 gtcgcacggc gccacgcatc tgctcgagaa gaagctccgc gcgccttgac ggggtcggct
    15121 gcggcggcgt ctaatgggcc gcgcgccgca cggcgcgcgg cgaaggagca gggcatgcgg
    15181 gagcggttcg tcgtcgagac aggtggtcgc cacgccgcgg gccggctgca cggagcgcag
    15241 ctcgccgcga cgcgcagcct cttcggccac gcccgttccg gccgtttctt cggcggcctc
    15301 tggttcaaga tcatcagcga acgcggaccc tgctgacgcc acagcagcac ggccgccgga
    15361 ggggcgcacg cagcgcgccg gcccggcggc cactcaccgc tccgccaccc gaagaccgac
    15421 ccgaagggga cctgccgtga accgagccac actcagcgtc tgggacgacc gcgcctgccg
    15481 gcgcgtccac gaagcctcgc tgaccgtgct ggagcgcacc ggcgtcgacg tgaaggacga
    15541 tcgcgcccgc gagctccgcg ccgccggcgg cggcacggtc gacggccggc gcgtgcgcct
    15601 gcccgccgcg ctggtggagc gggcgctcgc gagcgcgccg cacagctgga cgctgcgccc
    15661 gcgcggcggc gagacggcgc cgctggagct gcgcgacggc gccggctacc tcggcagcgg
    15721 gccggactgc ctctacgtct ccgacccgga cagcggcgcg cggcggcgct cgacgctggc
    15781 cgacgtcgcg gcggcggccg gcgtggtcga ggcgctgccg aacatcgact tcgccatgag
    15841 catggcgctg ccgcgggacg tgcccaacga ggtggtggac gtcacgcagt tcgccgccat
    15901 gctgcgcggc acgcgcaagc cgatcgtcgt ctcgagcccc ttcggcggcg agacgctgca
    15961 caccatgcac gcgatggcgg cggcctgcgg cgaggcaggc agcttcgcct gcctggcaat
    16021 gagctcgccg ccgttgcagc tggacgacgt ctgctgcacc aagacgctcg cctgcgcaga
    16081 cctggacgtg ccgctggtgc tcgcgccgtc ggtgtcggcc ggggcgcagg gcccggcgtc
    16141 gctcagcgcc tgtgtggcgg tggccaacgc cgaggtgctc gccggcctcg tcctgcacca
    16201 gctcggcaag gccggcgcgc cgttcgtgat gggtgtgggc accggcgtca tgaacatgcg
    16261 gacggcggtc gacgtctaca acgcccccgg ggtgttcctc ggcaaccagg cgcacctcga
    16321 cctgatccgc tggtacgggc tgccgagctg gcactacgcc ggccactcgg acagcaaggt
    16381 cctcgacgag cagtgggccc tggaactggg gatcgcgacg gtcatgggcg cgctctcacg
    16441 cgccacactg ctgcacgacg tcggctacct ggagagcggg atgcagagcg cgctcgaagg
    16501 gctcgtgctc ggcgacgagg tcgccgggta cgcccgagcc ctgctgacgg agctgccggt
    16561 ggacgacgag gcgctcgcgc tggccgaggt cgaggccgcc ggccccggcg gcactcacct
    16621 cggcacccgg atgacgcgcc ggcgcttccg cgacttctgg cgggcgtcgc tgatcgacca
    16681 gaccacctac gagcgctggt ccgcagccgg cagccagacg ctcctcgagc gtgtgcgcgc
    16741 ccgcctcgcc gagatccggg cggcgggccc ggcgttcgcg ctggacgacg cgacgcagcg
    16801 ccgcatcgac gccctggcgg cgggccgccg cccctgacga caccgcggcc gcaagggccg
    16861 ggctgctcgg ccgccgtcct cgtgtagact cccccgcggg cgcgctgcgc gcacgacacc
    16921 ttggagggac gatgtccgtc atcaaggccg aacacctcac gaagcgcttc ggcaagaagg
    16981 tcctcgccgt cgacgacgtg agcttcgcca tcgaggcggg cacgatcacc ggcgtcctgg
    17041 gacccaacgg cgccggcaag acgacgacgc tgcgcatgat cctcgacctg gtgcggccga
    17101 cctccggttc ggtgacgatc ctcggcagcc ggtaccggga gctgcgcgcg cccgccgcca
    17161 gggtcggcgc gctcctcgac gcctccggct tccacccggg gcgctccggc cgcaacgcgc
    17221 tgcgcgtgat cgccaccggc gccggtatcc ccgacgcgcg cgtcgaagag tccctcgagc
    17281 tcgtcgagct cgaggatgcg gcgaagcggg cggtcaaggg ctactccacc ggcatgaagc
    17341 agcggctggc gctggcggcg tcgctgctcg gcgaccccga ggtgctgatc ctcgacgagc
    17401 cggccaacgg cctcgatccg gaggggatgc tctggctgcg cacgttcctg cgcaagctcg
    17461 cggccgaggg caggacggtc ctggtctcca gccacgtcct cgcagaggtc gagcagacgg
    17521 tggacgacat cctcatcatg agcaagggcc ggctgctcgc gcaggggccc atccacgagc
    17581 tcacggcgtc cacgcagccc gttgtgcgcg tgcgctcgcc gcaggcggac aggctggagg
    17641 tcgcgctcaa gtctgccggc gcggccgtca accgtctgga cgacgctctc gcgatccggg
    17701 ggccgtccgc cgccgaggtc ggcgacctgg cccacgcccg cggcgtcgcg ctccacgagc
    17761 tcctcaccga acagcccagc ctcgaggagc tcttcctgca gatcgccggc acgaggaggg
    17821 agcagtgatc gccctcgccc gggccgagct gctcaagctg cgcagcacgc gcacggccct
    17881 cggcctgttc atcgtcatcc tggtggtgac ggtcgtgccc ctggtcgggc tcctgatctt
    17941 cctctccccg gcggacctcg cgtcgggagg gctgggcgcc atcgccgtct cactggtgcc
    18001 gctggtgctg ctcgtgttcg gcatcctcgg catgacgaac gagtaccggc acggcacgat
    18061 cacgtacacg tatctcgtga cgccgcggcg ctggcaggtc atggtgacca agctcgtcgc
    18121 ctatgccgtc gtgggcatca tcacgatgct ggcgacggtc gtgctggtct acgtcgcgat
    18181 ccgcctcggc ggcgcgatcc gcggggtgga gatcgacgcg atgggcgagg cgaccctcgg
    18241 cgactacacc cgtcagatcg tggtcgccgg gctcatcacc accttcggcg tcgcgctcgg
    18301 cgcgctgctc cgagcccagg tggtcaccgt cgccgccgcc ctcatctggg ccctcttcgt
    18361 ggagaacatc atcgtcgtgc tgaagcccgc ggtgggcgcc tggctgccct tcaccgtgtt
    18421 ccagcaggtg ggcgccggcc agacggtgga cgcaggcacc ccggcggcct ccgagatgct
    18481 cacgcgcccg gccgccttca tcgtcagcct cgtctacatc gccgtcgtga gcgtcgcggc
    18541 ggtcttcatc tcgctgcgcc gcgacgtcac ctgaggccac cggtggagca cttctccatc
    18601 cgcggcccca tgctcgggca gggcgaggcg gccgagcgca tcctccgcgc cctcccgggg
    18661 tggttcggca tcgagtccgc gctgctcgac tacgtccgcg ccgcggacga gctgccgacc
    18721 ctcgtcgcgc taccgcccgg aaccggccgc gccgggcggg aggtccgggc ggacgaggtg
    18781 atcgggttcg tcacgctcaa ggagacgagc gaggacgcct tggaactgca cgtcatgggc
    18841 gtcctgcccg cgtggcgccg gcgcggggcc ggccgcgcgc tcgtcgagca cgcggccgca
    18901 tactcccgcg ccgagggctt ctcactgctg cacgtgaaga cgctcgcgcc gagcgacccg
    18961 gacccggggt acgcggcgac gcgcgcgttc tacgccgccg ccggctttcg cccgctcgag
    19021 gaactgccgc aggtgtgggg gccggagaac ccctgcctgc tcatggtccg gccgctctag
    19081 gcgcctgccg cgtcgagcgc cgccagcggc gtcggcgccg tgtaggtgcg cgccgcgagc
    19141 tccttgtcga gctggtaaag gcccgccggg tgctcgccga ccatcttgaa cttgcccacc
    19201 cagtccatgg ccgtctcctc ctcctcgacc tgctcggcga tgaaccactg gaggaagttg
    19261 tacgtggcgt ggtctttgag ctcgagcgcc agcgaggcca tgtcgtggat gcgcgccgtg
    19321 accccctgct cgtgctcgag cgcctcctgg tacatctcca gcccgcccgg ccactccgac
    19381 tcgggccgat cgatcgcctc caggatcacc ctgccgcccc gggtgagcac gtagctgaac
    19441 atgatgcgcg cgtgggccag ctcctcctgg tactgcacgg tcatccagtt ctgtccgcca
    19501 gacatgccga ggtccgcgaa cttgctcgcc atcgagagat agaggtagcc cgagtagagc
    19561 tcccagttga tctggctgtt gaacgcgtcc tgcatcttct ggtccagcat gtcgccctcc
    19621 gtcgctaggt gcgggcctga gcggcccggg tgcctgtccc agctatctac ccaatcgcga
    19681 gggagtgcgc acggggacgt cgaagggaac actgactgtg actcatccct tcgatcggag
    19741 ctcacgatga acagagcgtt cgtcaaggag ccggacgagg gcgcgcctgc cgagggtctt
    19801 cccgagcggc agatcagcga gcatccgaac tacgtgacgc ctgcgggtct gcgccagctc
    19861 gaggccaggg tgggcgagct cgagacacgc cggctggaac tgctggaaga ggacgacctc
    19921 gaaggagagc tcgcctacgt cgaccgtgac ctgcgctact acacgacgcg gctcgagagc
    19981 gcgatcctcg tggaccccga ccgtcagccg caccgcaagg tggcgttcgg cgccgccgtc
    20041 accgtgagcc agaacggcgg cgacctgcgc accttcacca tcgtgggcga ggacgaggct
    20101 gacctcaaag agggcaagat cagcttcgtc tcgccgctcg cggaggcgct gctcgaagct
    20161 cgcgtgggca agacggtgct gtggcggcgt ccggccgggg acctgaagct caccgtcgag
    20221 gagatcgact acccggcctg agcggtgggg cgcgtcgtcc gcgcacgaag gtggcctgct
    20281 ctactcgagc acgaccgaaa ggtacgccgg cgtcggctcc tgccccagca ggaggccggt
    20341 gacgcctttc tgcgtgtccc agccgtacga gcgcgtggga ttctcgtcgc ccatcaccgc
    20401 gagcgcccac cgcttcttgc cgttctccag ccaggccgcc tgggtcatga gcttgttgtc
    20461 caggcccagc cagccgccct tgaagaagct cgtccagccc tcgggacccg ccgccgccgg
    20521 gatgccccag cgctggatgg ggatcactcc cgcgagcagc tcgcgcgcca gggcacgccc
    20581 cttgggcggc acgagggact cgagctgcca gaagaagcgc gcctggtcgc gggcggagtc
    20641 ccgcgtgtcg atccagccgg agccctgctc gtagtcctcc aggccggcgg ccttggccac
    20701 cgcgcgcatg cccttggcgc cgacctgccc gaagaccgtg ttcgcggcgc cgttgtcgga
    20761 ctcggtgatc atcttcgtga gcgtcgcctc ggtcgccggg tccggcgtcg ggtcgtcgcg
    20821 cagggaggcg accagcagga tggccttgga gaggctggcg agctggaagc gctcgtgctg
    20881 ccggtggccg ccggcgtcct ctccgttggt gtccacgacg gcgaaggcgg cgtcgccgtc
    20941 gcggcccgag atccactccg aggccgccgc gacggcgttc tcgccgggga agccaggtgg
    21001 cagcggcttg cgcacgacga gggtcgcgga ggccacgcca tcggaggcct gggccgtgga
    21061 agggctcggc gaagcgagac cctcgtcccc cagccggagc tcgtaactgt agcggcccgg
    21121 cgggaggtcg atcggaacga cggcctcgag tctctcgccg ggctcggcga cctccgtcag
    21181 gcgccgagcc ttgacctgct gcccgccggc gtcgcggacg acgagggtca ccgccgtcgt
    21241 ccccgcggcg gcgacgacgc ggtaccggaa cgtcgccttg tcgccctggc gcacctcgac
    21301 gtcggccagc cgagccagcc gcgccggtga cggtgacggc gccgaggacg gcgcggcgct
    21361 cgcggctgcc ccatcggccg accccgagcc gtcgcgcaga gcgaacgccg cagcgacggc
    21421 cgccgccgcg agcgcgagga ccgcgaccag agcggcaagc ttggcggcgc ggagacgacg
    21481 cctgcgccgc cccctcgccg attcgtagat cgtccgccgc tcgctcatcg cgccatccta
    21541 acgcgcgcgc cgccggccga ggcgggatcg gcggcgctga agccgcgggt caccgtccgc
    21601 tcaggccggc gatcaagccg gcgctcacgg gtgatcgcgg gtgagaatgg cggagcgaca
    21661 tgggagtcga acccacccag gacgggatca ccgccccgca gacggttttg aagaccgcgg
    21721 cagtcaccgg accacgagcc gctccactgc gcatcttgcc acgcgggagg ctccaggtca
    21781 cccgagggtc cccgaagacg ggctccgacc gccgtgcccc gtacacgcca ggactgttag
    21841 tccacgccct gcgggggaac gcgtgaacca tgggaggagc cgagttcgat cagcaaagca
    21901 cgggagactg accaatggta cgggagatag tgcgcgcgga acacgcgcat gcggcgcggc
    21961 gagcggagca gcggtcgcgg cggcggggct acccctctcc ggtcgcgaag gaactgtcag
    22021 cgcggcgagt ccgcgagaag tacgtgcagg cggtccgtcg agtacagggg cggtactgtg
    22081 aacggctcac cgtcccgacc cggagcgttc gaggcggcgc ctgcagggcg ctctcggtcc
    22141 tgagctgacg acgcggccgg cgttaccctc gccggacgcg accgagagcg gcccgcggcc
    22201 ggccgcctgg cgagaggcgg ccggccacgg gcctgcggct tgtcccgggt cctacttcgc
    22261 caggtcgagc agcgcctgac ggtcgtgggc gagcttgtac tcggactcca tctccagcag
    22321 gccgccgggg aacccgacct tcggcatcag cgccacgtag tcgtcggcgt tgaaggcctc
    22381 ggggttgtgg acgccgctct tggggtcgcc cggcttgctg ccgaggctgc cggtggcgag
    22441 gagctcgagc gtgatgacgg gggtgaaacc ggtctgcgcg gtgaccacct ggacgccgac
    22501 ccgctcgagg cactcctggt tgtcggcgac ctggtacagg tacaccttgc gctccatgcc
    22561 gtccttcttg ccgacgacgt aggtgccggc cgccgtcttg ccgacgtact tcttgccgat
    22621 ctcgttgggg tccggggcgg cggcggcgat gacgtcgcgc ggcgcgacct ccacgttgcc
    22681 gaccttgatc ttctcggcct tgtccatgtt gatggagcgc agggccgaca tgaagtccat
    22741 gaactcgtcg ccgagggcgt acttgaactc ggccttcttg acgcccttga gcctgtcggc
    22801 gttgcgcccg atctggacga cctcctcgtg ctccacgtgg ccgacctcga cctcgccgat
    22861 gccctcggga aggtagaact tctcgagctc cgcgaacggg cgcacggtgt ggaagtcgcc
    22921 gttctcgaag acgatggccg ggttgttgca ctcctcgatg gtggtccaga cgctgaagcc
    22981 gaagctgatg ccctccgcgc cggggatctc gaggttgctg ccgtcgcgga tgccgatgta
    23041 gtcgacctcg tcgaagaggt ggtcggcggc gtagcgggcg aagaagtcgg ccatgccggg
    23101 ctcgacgccg aagccggcga gcgcgtactt gccgatcgcc tcgaactgcg ggctgagcgc
    23161 gtactcgtcg tcgccgagct tcttgcccgt cttgttgaac gggtccgtgg ggtgccactc
    23221 gctcagcgtc atcgccgtgt cgaggtagtg caccttcgcc ttgagcgccg cctgcaggag
    23281 cggcgggttg aagttcggcg cgcagaggtt catgaggatg tcggcgccgt gcttcgcggc
    23341 cagcgcggcc acggcgtcgg cgtcgctcgc gtcgacctgc tcggcgacga agcgcggatc
    23401 cgcgagcgtg gcgacgaagt cggcggcgcg cttgccgtcg tagtcggcca tcaccatctt
    23461 ctcgagccag gcgccctcgg ggtcgcgctg tttggcgatc gaggcgatcg actggcccac
    23521 accaccggtg ccgacaacca gagctttcat gacgttccct ctctttgacg tggcttgcgc
    23581 gcgccggagc cggcgcgccg ccctggagga ggcgtgagac gacagctcgg cgacccctcc
    23641 aaggcagttc gtgaggcaga ttgtataatc ttgaggacgc cgcgcaagcg ttaagttagc
    23701 aaccgtggag gtttaggcgc cgaccctgta catcgtgcgc tcgccgaggg cggtgacgac
    23761 cttggcgtag ccggcgagct ccgcgtcgag ggcggggtcg cccgtgtcca ccagaagcgg
    23821 ccggccctcc aggctgagca gcttggtctg cgtggcgatg acgacgatgt ggtccgtgcc
    23881 ggccgcgcgg atcacggcgg ggctgatctg ctggttgccg cgcccgaaga tgtgcccctg
    23941 cccgccgatg acggtgacga cgatggaggc ctgcccggcg gcggcgatga gggcgagcag
    24001 ctcgccctcg gtgaggtcga cgccggcgag ctcgccgtcg cgcacggcgt cgacgccgag
    24061 gagcgtcttg ggcagtccca ggcgctccat gacggtgcgc gtcgtggtgc cgggcccgag
    24121 gatgtacagg gcgcccggct ccatcccggc gatcacctcg gtggcgatgt cgttgagggc
    24181 gcgctcctcg ccggcgacgc ctccggcctt ggcgctctgc gtgaggccgc gcgcgtaggg
    24241 caccgtcatg tagccgtaga ggtgggcgga gacgcggttc tcgcggaacg cctcctcgtc
    24301 gatgtccatg acctccccct cgcggagctg gacggcggcg gggcgctcgt acaggtacag
    24361 cgccgcgacg tcgccggccg ccgccggcgt cgtcgcgtac acggcggagt ggatcttgac
    24421 gccggcgggg acgccgatga cgggcaccgc gtcgccgacc gcgttgcaga tgttccgcgc
    24481 ggtgccgtcg ccgccggcga agaggatgag gtccacgccg gcggcgagca ggtcgcgcgc
    24541 ggcctgctcc gtgtcggccg gcgtggtcag gacgaagtcg tcgcaggcga tggcaggccg
    24601 ggcgccgctc cgctcgagct cgcagagcac gtacgagcgg cgcccgtcga acgatccgag
    24661 cacgcgcggc tcccagccgg cggcgcgcgc ctcgtcctcg cccatctcgc ccggccaggc
    24721 gaggacctcc acgtgctcgc gcacgcgtcc caggcgctcg agcgcctgac gtgcgcgccg
    24781 cggcgcgtcg cgcacggcgc cgagctcgag cgcccggcgc agcacgtccg ccccgtcgct
    24841 gcccttgagg ccgacgcggc cgcccacgcc ggcgaccggg ttcacgatga ggccgagctt
    24901 cttcacgccg gggtcaccgc gagaccagac ggctcagcgg tgcttcttgc cgcccttgcg
    24961 ctgccgggac ttggtgggag ccgcgctcgc cgtgcgcccg ccgccggagc cgccccgcgc
    25021 gccgcccgga gccgtcgcat agcgcgccgc cgggcgcttc cgcgcgatga acaggtagac
    25081 gatcatcgcg ggcaccagga gcgggatgac gagggagttg ccgcccagca ggctcgccgc
    25141 cagcgcgccg aggacggcga tgaacacgag cgagcggaac atcttgacgg cgtagggcga
    25201 catcttgcgg ccgcgcaccc acaggtccgc ggcgtcgatg agcatcgcga acgcggcgac
    25261 cgtggcgcag agcgtcgaga aggccgcggc cgccgtctcc aggctctcga acacgccacc
    25321 caggccggag aggaacagcg ccgtgagaaa cgcggccgtg gcgaggaaca ccgtcagccc
    25381 gaacgcctcg aggacgcgga tgccgggctt cgccggttgc tggggcacgt ggtccgcctt
    25441 tcgtctggtc gccgagaccg gccgcgaagg gcacgtccgg tctcgggcca tgatacccga
    25501 gacgcgcagg aggcgggggg cgcgagcgtg cgcccccgcc cccgcagcca tcgagcgaaa
    25561 ccaccatatg ccctctcgcg actggcgtca tgggtggcat catgggtggc accttgcacg
    25621 acgtcatgag tggcaccatg ggtggcgcca ccggtggtgt caccgccgct cacgcacccc
    25681 caccgcgggc ccagacggag tcccggcggc ggcggagccc ggcggggtcc cagacgcgag
    25741 cggggcgggg gcacccgccc cgccccgccc tcgcagcccc ggacgagatc agctcggggc
    25801 gttcagacct tgtccttgaa cttgcgcttg tacttgcgcc aggtgaaggc ccactgctcc
    25861 gggtcctcca tggcctcgag gcgc
//
LOCUS       DASUMX010000263        16051 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_957324, whole genome shotgun sequence.
ACCESSION   DASUMX010000263 DASUMX010000000
VERSION     DASUMX010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 16051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16051
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_957324"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..894
                     /locus_tag="VFZ86_12535"
     CDS             <1..894
                     /locus_tag="VFZ86_12535"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX5643143.1"
                     /translation="TISDNVVFAQLSIDVGPESTVDIAHRMGITSPLDAVPSITLGTS
                     RVTPLEMADAYATLAAYGVRHKPQAIVKVLVPHDGRYKVDWKPKTTGKRAIPSGVASV
                     VTECLERVASGGTGSLTGSYFPDPRAGKTGTTENGWDVWYVGYTPKLAAAVWMGDLEK
                     SSPMNSAYGGTYCAPMWAKFFAAALKDADDHPSFKDFPWTFTPWEGKMQAMSPSASPS
                     ASGSATPSPTATRTITPKPTATKTVTPTPPPTPTKTPKPTPTATSSAAAKKVVTTAGV
                     GGGGSGLAGAVFNALAGLLGI"
     gene            complement(931..1539)
                     /locus_tag="VFZ86_12540"
     CDS             complement(931..1539)
                     /locus_tag="VFZ86_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HEX5643144.1"
                     /translation="MSSAVLEQFRAVGRDLFVAGAISSHGGNLSVRMGDRILITRRGS
                     MLARLEERDVIETGLEENDANVMLASTEINVHRAIYLGTAAQAIVHAHPPYAIARSLM
                     CDEIIPIDSEGSYLLHKVPVVHTELTAGSKEVAELLPRWLKEYDLVMLRGHGPFAIGH
                     LLEEAYQLTSVLEMSCRIMTIADGLGEQVREYRKGSERYETW"
     gene            complement(1536..3464)
                     /locus_tag="VFZ86_12545"
     CDS             complement(1536..3464)
                     /locus_tag="VFZ86_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HEX5643145.1"
                     /translation="MTPPASALRPLRGLRLDLSRRTCDEEQLPAGVARAWLGGRGLGA
                     CLALRERLFEVEPLAPENLLIFAPGPLTGTGAPASGRYSVTGRSPLTGTVFDGNSGGN
                     FGNALRRLGWDYLIVTGALEEPGYVTLGTDDARGEAPAGAPEPVAGAQPLGGSGGATL
                     HPAAGLWGLDVPASLERLRGLHPGSEAAVIGPAGERGVLFASIVNNRGRSIGRGGLGA
                     VMGAKRLKGLVVDGHGRRTPPVADPERLEFIVYEAEKLLKSNPITSTALPEFGTAVLV
                     NVLDQAGALPTRNHRESRFEGAAAISGEALKREHVHRRSACRGCIIGCARRTTAGGES
                     GEGPEYESIWAFGAECGVSDLTAIVQANYACNRAGMDTITMGVTIACAMELTDLGLLP
                     GGPRFGDARAIIDLAEATAAGEGLGAELGLGSARFAALHGRPELSMSVKRLEMPAYDP
                     RGMKAQGLAYATSNRGGCHLRANMLGPEILGIPKMVDRFATLGKAGLLINIQNLNAVL
                     DSLSVCKFTAFAMKEDYFARQLSAVWGEPVEPQELLLLGERIWNAERIFNLAAGFTRA
                     DDTLPPRLLHEPVPAGPSAGQVVDLPPMLDEYYISRGWDGGGTPSAAKLERLGLSEVA
                     AAAGVAPGTPRAEGTAAR"
     gene            complement(3490..4539)
                     /locus_tag="VFZ86_12550"
     CDS             complement(3490..4539)
                     /locus_tag="VFZ86_12550"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5643146.1"
                     /translation="MTLRPLTAADAADVFAVFAAPAVMRYWNSPPPKDLADAGEWAAH
                     LEDMQRRLGYAQWRVAERAGDRLVGIAGLQPLDGGPEVELTYALEPSAWGRGYATEAG
                     AAALEYGFGEAGLERVVAIAREENRGSVAVLRRLGLRPLGAARYWGKPWAKYALPASA
                     WREERAAATPPLRTERLELRRFAAADRDPLAGVFGDPAVMRFVGAERRPLDEPQLAAA
                     LAVAGQHWEQLGYGPLAVVERASGRLVGEAGLQPLDGGPDVELTYTFARAAWGRGYAT
                     EAARAVLRWAFAGLLLPRVEAVADPGNHASLRVLEKLGMAPLGPRECYASVLAEWGMT
                     FGAWRELAGPAPARG"
     gene            complement(4568..5917)
                     /locus_tag="VFZ86_12555"
     CDS             complement(4568..5917)
                     /locus_tag="VFZ86_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012031644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HEX5643147.1"
                     /translation="MSDVAEEHLTRDAAGGLFWQPAAETLPVPQLGELQMERLRATLE
                     NACENVPFYRKKMEELGVKPGDVTCHGDVADLPFTTKIDLRDHYPFGMFARPRDQVVR
                     VHASSGTTGNPTTVGYTRGDLDVWADLIARTLAAGGVAPGDLLQNAFGYGLFTGGLGL
                     HLGAERLGCTVVPISGGNTERQLKIMRDFGATALSCTPSYAMYLADAARDRGLRPEDL
                     PLRVGFHGAEPWTNELRVQIEAGLGEHALDIYGLSEMGGPGVGYECLCKAGMHVNEDH
                     YLAEIIDPETLRPLPYGEVGELVFTTLTRQAQPLIRYRTRDLCTLIAEPCACGRTSAR
                     MSKPIGRSDDMLIIRGVNVFPSQIEEVLMSIPAIEPHYQIVVDRKGYLDVIEVWVEVG
                     EDIFAETMGALERFQRNVQAQIFNVLNIQVAVKLKEPKAIQRSEGKAVRVVDRRKAL"
     gene            complement(5914..6327)
                     /locus_tag="VFZ86_12560"
     CDS             complement(5914..6327)
                     /locus_tag="VFZ86_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX5643148.1"
                     /translation="MKVTQLTVFLENRSGRLAEVADILGRAGINIRGFSTTEAAEYGI
                     VRLILTDPQRARTLLHDAGFTTHFSEVLCVKVEDVPGGLAGVLDQLAEAGVSIDYLYS
                     ISFWNICFAVRDIDRAVELLAGKVTLLSDEEVRAL"
     gene            complement(6324..6920)
                     /locus_tag="VFZ86_12565"
     CDS             complement(6324..6920)
                     /locus_tag="VFZ86_12565"
                     /inference="COORDINATES: protein motif:HMM:NF013707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HEX5643149.1"
                     /translation="MNGTTTVSLVGVGGQGILLGAAILAETAAAAGLEVKASEVKGMA
                     QRGGSVLSTVRFGKHVWSPVAPRADVVIATELLEGIRGLGLLGPKGTLVCAFTTRITP
                     GSVLRREEGYPDDVSGAAARRGVRLLTVDAEGVAREAGTVRAVNVALLGAASTVLPFG
                     EEQWRRGLEAAIPAKILEVNLRAFELGRAAVASQEVES"
     gene            complement(6917..8704)
                     /gene="iorA"
                     /locus_tag="VFZ86_12570"
     CDS             complement(6917..8704)
                     /gene="iorA"
                     /locus_tag="VFZ86_12570"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HEX5643150.1"
                     /translation="MERQLFFSGNEAIAHGAVRAGARFACGYPGTPSTEIIEATAGLE
                     GIHSEWCVNEKVALETAVGASLAGVRTIVTMKHVGLNVAADPFMTLSLTGVNAGLVVI
                     TADDPGMHSSQNEQDNRNFAKFAKVPMLEPSDSQEAYDFVAEAFKLSEEFDTPVLLRT
                     TTRISHGRGQVLPRVPVEVEPREYARDISKYVMVPQYARQRNRVVLERLEKLRRRAEE
                     SPLNVLEAGTSDLGIIASGVAYAYAREAFPKAWFLKLGMSHPLPYAKVARLYEHVSRV
                     AVVEELDPFFEEQVRALGLPVIGKAAIPADGELDQELVFRALEPYVQRKPPRRRRPVM
                     PPPPRLATPPKPAAGLPVRPPVLCPGCSHRSVYAALRKRRLAPMGDIGCYTLGALPPL
                     LSLDSCLCMGASIGMMAGFNKALGRKAAVGVIGDSTFFHSGITPLVDAFYNEVEGMVV
                     VLDNRTTAMTGHQGHPGTGLHPDRSQSPTIDIQKVCEGIGVRCVTVDATDHKALDAAI
                     KDEAKAPGLGVIVALAPCVLVTREVAGEVVVDEERCSLCGVCVDLGCPALAPGDDAVS
                     VLGTCTGCGLCVEVCKRGALSLACAGDET"
     gene            8925..9920
                     /locus_tag="VFZ86_12575"
     CDS             8925..9920
                     /locus_tag="VFZ86_12575"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX5643151.1"
                     /translation="MSRPSRRRRHPALLRARSRTLFFLLLTALALALGYRAAAAPAAA
                     GALLGLVSIRVARKHPAELEHTFVVLDWALLGMALVLSGGAGSWLLVTVPFLTLGQLA
                     GTSVGEWPFLAGPSLLLLVVLAIADPSLGGSRIGAVAKLAVLVAGGCVAATRLRRRTA
                     PRARRAHAPKVDVSTGLSTGTCLPAFLESAAETALAEHQPLSVVYVRLEHYEDSRNFL
                     GAEGSEGLVRGVARRAERSLCADGRAFRVRPDGLVLVLPGLSLAEARQTASDLAREVG
                     GSLIAGRRQTLATGAASFPTLRSIEELLGAARDEALPEAVSTEVAGTVVPLAAAQ"
     gene            9980..10279
                     /locus_tag="VFZ86_12580"
     CDS             9980..10279
                     /locus_tag="VFZ86_12580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643152.1"
                     /translation="MEIDITSQQVPEGVAVQVKVRLSGRETSRLFVSGDTLVQLPLDG
                     ALPDLDGSPVPRMSIYLSELAGRRDGLTRTFAGEAAAEAFTAAVRTQLETALEAS"
     gene            10276..10617
                     /locus_tag="VFZ86_12585"
     CDS             10276..10617
                     /locus_tag="VFZ86_12585"
                     /inference="COORDINATES: protein motif:HMM:NF015232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HEX5643153.1"
                     /translation="MTDQADILAAQLAPYRETPGIVAALLVSRDGFVVAADADAGFKA
                     DAVAAQVAGVIDIGARLAAELGQPEARYISVDFDGLNLVLAPFGDELMLVLAGLPSAL
                     LCEYRLIKPGA"
     gene            10662..11255
                     /locus_tag="VFZ86_12590"
     CDS             10662..11255
                     /locus_tag="VFZ86_12590"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEX5643154.1"
                     /translation="MEDAVEQLENLARSVGECTRCEQLVASRLRAVPGGGHPHCAVMV
                     VSLASDPADEADGRLAGEALLAGLAEFMPALAESRDQVYVTALAKCVPRSDGAVRAPE
                     QQELENCYPYLSKELTITTPHYVLTVGEETTRFLLGKLFKHRPYEPGDSLELRLFDNP
                     SFKVVPVAAPEDLRQRDAKARKEYTERLRALGQVMGL"
     gene            complement(11295..12185)
                     /locus_tag="VFZ86_12595"
     CDS             complement(11295..12185)
                     /locus_tag="VFZ86_12595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX5643155.1"
                     /translation="MTVTGRRLAAIFNPHSGGGGYKRDLPLIFDALRGLGYRVEGLET
                     REPGDAGRLAREAVADGVDIVAALGGDGTVNETINGLAGSDVPLAIIPTGTVNVLAME
                     LGIPLEPPDAVKLLASGNVSWIDLGRAGDRYFGLMAGVGMDAAVVASMHPAMKKALKE
                     AAFAVQGVGTYLTHDDPLFRVTARERTVEGYFAVFGNSSNYGGAFGITPLADMRDGLL
                     DVCVLKDKSFVSTVWYWTAALINAHIKHPKVEYFRTESAVVEAVEGDQEVLVQTDGEM
                     AGKLPMECRVVPRALRVVVP"
     gene            complement(12182..12997)
                     /locus_tag="VFZ86_12600"
     CDS             complement(12182..12997)
                     /locus_tag="VFZ86_12600"
                     /inference="COORDINATES: protein motif:HMM:NF005596.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HEX5643156.1"
                     /translation="MIADYHIHTGMCRHAEGEAREYVERALELGMTAMGFSDHLPFLG
                     GWEPRHDLTDDWAMPLDELDDYVTLVQDLAREYAQDLRIVLGIEADFIPETMEDTAAV
                     LAQYPFEYVIGSVHIVGDRFGFDHPEMEGRLGAYGIGRIHLESLDLTRQAAESGLFDV
                     AGHLDHAAKFGPPEDAEAVAAAASAALRAVAAAGMALEINTGGLRKPIGRAFPAPALL
                     AEAGGLGIPLVLGSDAHRPGDVGHGFDHAAGLARAAGYTALRSIASGEQEPLP"
     gene            complement(13039..13740)
                     /locus_tag="VFZ86_12605"
     CDS             complement(13039..13740)
                     /locus_tag="VFZ86_12605"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX5643157.1"
                     /translation="MIPLHDDNPVRRFPAVTAALIAANAAVFVVQLFLPRWGVTLEGW
                     YYLLGARPFELTHRVDIPPFGWFPWWATLFTSLFVHGGWLHLIFNLWYLWIFGNNVED
                     AMTRPRYVVFYFVCGMAATTAQVLVAPTSDVPLIGASGAIAGVLGAYLVLFPRARVLT
                     VIPLIIVWPVFEVPAWILLVVWFVLQGVGGAQSYGSGEAGVAFFAHLGGFVAGMLLVL
                     LFARRRRATGARRRV"
     gene            13926..14183
                     /locus_tag="VFZ86_12610"
     CDS             13926..14183
                     /locus_tag="VFZ86_12610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643158.1"
                     /translation="MPGTTQSAEPRVIVIPRQLEGFFYERLTAEFAARDDVRVVVDRR
                     VGERRRPRWGSGAGPFTERRGGDRRAQSPAWSLPDMPFSAS"
     gene            complement(14259..14843)
                     /locus_tag="VFZ86_12615"
     CDS             complement(14259..14843)
                     /locus_tag="VFZ86_12615"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5643159.1"
                     /translation="MAKRILVVDDEPDIRRLVAEALEVTGYDVHTAANGGDALRTAGL
                     YLPDLVLLDIMMPDMDGFAVYERLREKPCELRSPVIFLTARREINDKLLGFEKGAADY
                     ITKPFHIKELLARVKVHLGELGPPRGDVPNPMTDRELEVLGLLASGKTYKQVARALEL
                     SQSTVRNHLHNVYHKLNVVDRAQAVIVSRENGWI"
     gene            complement(14935..15114)
                     /locus_tag="VFZ86_12620"
     CDS             complement(14935..15114)
                     /locus_tag="VFZ86_12620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643160.1"
                     /translation="MNWVILIAGVAGLLVAGYGLVTLTTHAVRARRYGDIAIGVAVVV
                     AVTVLLVAFGDRLIR"
     gene            complement(15149..>16051)
                     /locus_tag="VFZ86_12625"
     CDS             complement(15149..>16051)
                     /locus_tag="VFZ86_12625"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HEX5643161.1"
                     /translation="RLAALAAPVAPLVNGLGRTRLARALARQAGVAAERPLPRFARRP
                     LTRRLSPVPGPQGEHAAARPAAALFVDCFIQYQEPRIGEALARLLAAAGLPLAAADAG
                     CCGRTALSTGQIEKARGHALRALDGLYPLASAGAEILFVEPSCLSMVRDDWARLLPHD
                     HRVAEVAAASRPALELVAGLAAEGRLRFRAGGRALLHSHCHEKALGFAAATAAALAAV
                     PGLEVEDPDAGCCGMSGVFGYEARHYELSVAMAERALLPAVRAASPETVVLATGTSCR
                     TQIGDLAGRTALHPLEFLAARLQP"
BASE COUNT         2222 a         5842 c         5844 g         2143 t
ORIGIN      
        1 acgatctccg acaacgtcgt cttcgcgcag ctcagcatcg acgtcggccc cgagagcacg
       61 gtggacatcg cccaccgcat gggcatcacc agcccgctcg acgccgtgcc gtccatcacg
      121 ctgggcacct ccagggtcac cccgctcgag atggccgacg cctacgccac gctcgccgcc
      181 tacggcgtgc gccacaagcc gcaggccatc gtcaaggtgc tggtgcctca cgacggtcgc
      241 tacaaggtcg actggaagcc caagaccacc ggcaagcgcg ccatcccctc cggcgtcgcc
      301 agcgtggtca ccgagtgcct cgagcgggtc gcgtccggcg gcacgggatc cttgaccggg
      361 tcgtacttcc ccgacccgcg cgccggcaag accggcacca ccgagaacgg ctgggacgtc
      421 tggtacgtgg gctacacgcc caagctcgcg gcggcggtct ggatgggcga cctcgagaag
      481 agttcgccca tgaacagcgc ctacggcggt acctactgcg cgcccatgtg ggccaagttc
      541 ttcgccgcgg cgctcaagga cgccgacgac cacccgagct tcaaggactt cccgtggacg
      601 ttcaccccgt gggagggcaa gatgcaggcc atgtcgccgt cggcgtcgcc gtcggcgagc
      661 ggcagcgcga cgccgagtcc caccgcgacc aggacgatca cgccgaagcc cacggccacc
      721 aagacggtga cgcccacgcc gccgccgacg cccaccaaga cgcccaaacc gacgcccacc
      781 gccacgtcca gcgccgcagc caagaaggtg gtaacgacgg cgggcgtggg cggcggcggg
      841 tccggactgg ccggcgccgt cttcaacgcg ctcgccggcc tgctcgggat ctgagctcac
      901 cggggcggta ggccgcagcg ccggcccgcc ctaccaggtc tcgtaccgct ccgacccctt
      961 gcgatactcc ctgacctgct cgccgagccc gtcggcgatg gtcatgatgc ggcacgacat
     1021 ctcgagcacg ctggtgagct ggtaggcctc ctcgagcagg tggccgatgg cgaacggccc
     1081 gtgcccgcgc agcatcacga ggtcgtactc cttgagccac cgcgggagca gctcggcgac
     1141 ctccttggac cccgcggtca gctcggtgtg caccaccggt accttgtgca gcaggtagga
     1201 gccctcgctg tcgatgggga tgatctcgtc gcacatgagc gagcgggcga tggcgtaggg
     1261 cgggtgcgcg tgcacgatcg cctgcgcggc cgtgccaagg tagatcgccc ggtgcacgtt
     1321 gatctcggtg ctggccagca tgacgttggc gtcgttctct tcgaggcccg tctcgatgac
     1381 gtcgcgctcc tccaggcgcg cgagcatcga cccgcgcctg gtgatcagga tgcggtcgcc
     1441 catgcgcacg ctcaggttgc cgccgtgcga gctgatcgcg ccggcgacga agaggtcccg
     1501 gccgacggcc cggaactgct cgaggacggc cgagctcacc gggcggccgt cccctccgcc
     1561 ctgggcgtcc ccggcgcgac gcccgccgcg gccgcgacct cgctcaggcc caggcgctcc
     1621 agcttggccg ccgagggcgt gccgccgccg tcccagccgc gcgagatgta gtactcgtcg
     1681 agcatcggcg gcaagtctac aacctggccg gcggagggcc cggccggcac cggctcgtgc
     1741 agcagccgcg gcggcagcgt gtcgtcggcg cgcgtgaacc ccgccgcgag gttgaagatg
     1801 cgctccgcgt tccagatgcg ctctcccagc agcagcagct cctgcggctc caccggctct
     1861 ccccacacgg cgctcagctg gcgggcgaag tagtcctcct tcatcgcgaa ggcggtgaac
     1921 ttgcagacgc tgagcgagtc gagcacggcg ttcaggttct ggatgttgat gagcagaccg
     1981 gccttgccga gggtcgcgaa gcgatcgacc atcttgggga tcccgaggat ctcggggccg
     2041 agcatgttgg cgcgcaggtg gcagccgccg cggttgctgg tcgcgtacgc gaggccctgg
     2101 gccttcatgc cgcgcgggtc gtaggccggc atctcgaggc gcttgacgct catcgagagc
     2161 tcggggcggc cgtggagcgc ggcgaagcgc gccgagccga ggccgagctc ggcgcccagg
     2221 ccctcgccgg cggccgtggc ctccgccagg tcgatgatcg cccgcgcatc gccgaagcgc
     2281 gggccgcccg gcagcaggcc gaggtccgtc agctccatgg cgcaggcgat ggtgacgccc
     2341 atggtgatgg tgtccatgcc ggcgcggttg caggcgtagt tggcctgcac gatcgccgtc
     2401 aggtcggaga cgccgcactc ggccccgaac gcccagatgc tctcgtattc ggggccctcg
     2461 ccgctctcgc cgccggccgt cgtgcgccgg gcgcagccga tgatgcagcc gcggcaggcg
     2521 gaccggcgat gcacgtgctc gcgcttgagc gcctcgccgg agatggcggc ggcgccctcg
     2581 aaccggctct cgcggtggtt gcgcgtgggc agagccccgg cctggtcgag cacgttgacc
     2641 agcaccgccg tgccgaactc cggcagcgcc gtcgaggtga tggggttcga cttgagcagc
     2701 ttctcggcct cgtagacgat gaactcgagg cgctcggggt cggccaccgg cggcgtgcgg
     2761 cggccgtggc cgtcgacgac gagccccttg agacgcttgg cgcccatcac cgcgccgaga
     2821 ccgccgcggc cgatggaccg cccgcggttg ttgacgatgc tcgcgaacag cacgccgcgc
     2881 tcgccggcgg ggccgatcac ggcggcctcg gacccggggt gcagcccgcg caggcgctcg
     2941 agcgacgccg ggacgtcgag gccccagagg ccggcggcgg gatgcagcgt cgcgccgccg
     3001 gacccgccga gcggctgcgc gccggcgacc ggctcgggcg cgccggccgg cgcctccccg
     3061 cgggcgtcgt ccgtcccgag cgtcacgtac cccggctcct cgagcgctcc cgtgacgatg
     3121 aggtagtccc agccgaggcg gcgcagggcg ttgccgaagt tgccgccgga gttgccgtcg
     3181 aacacggtgc cggtgagcgg cgagcggccg gtcacgctgt agcggccgct cgccggggcg
     3241 ccggtgcccg tgagcggccc cggcgcgaag atgagcagat tctcgggcgc cagcggctcg
     3301 acctcgaaca gccgctcgcg cagcgccagg caggcgccca ggccgcgacc gccgagccag
     3361 gcgcgcgcga ccccggccgg caactgctct tcgtcgcagg tgcggcggga gaggtcgagc
     3421 cggaggccgc gcagcggccg cagagctgag gcgggaggcg tcacttctcg agtatagacg
     3481 gcgcggcgat cagccgcgcg cgggcgccgg cccggccagc tcccgccacg cgccgaaggt
     3541 catgccccac tccgctagga ccgacgcgta gcactcgcgc ggcccgagcg gcgccatgcc
     3601 gagtttctcc agtacccgca gcgaggcgtg gttgccgggg tcggcgaccg cctcgacccg
     3661 gggcaggagg aggcctgcga acgcccagcg caggacggcg cgcgccgcct cggtcgcgta
     3721 gccgcgaccc cacgcggcgc gcgcgaaggt gtacgtgagc tcgacgtcgg ggccgccgtc
     3781 cagcggctgg agaccggcct cgccgacgag gcgcccggag gcgcgctcga cgacggccag
     3841 gggtccgtag cccagctgct cccagtgctg cccggcgacg gccagcgcag ccgccagctg
     3901 cggctcgtcg agcggccgcc gctcggcgcc gacgaagcgc atcaccgcgg ggtcgccgaa
     3961 caccccggcc agggggtcgc ggtcggccgc cgcgaagcgg cgcagctcga gccgctcggt
     4021 gcgcagcggc ggcgtcgccg ccgcgcgctc ctcccgccag gcgctcgccg gcagcgcgta
     4081 cttcgcccac ggcttgcccc agtaccgggc ggcgccgagc gggcgcaggc cgagcctgcg
     4141 caggacggcg accgacccgc ggttctcctc cctggcgatg gcgacgacgc gctcgagccc
     4201 ggcctctccg aagccgtact ccagcgccgc cgcgccggcc tcggtcgcgt accccctgcc
     4261 ccacgccgac ggctcgagcg cgtaggtgag ctcgacctcg ggcccgccgt cgagcggctg
     4321 gaggccggcg atgccgacca ggcggtcgcc ggcccgctct gccacgcgcc actgcgcgta
     4381 gccgaggcgg cgctgcatgt cctcgaggtg cgccgcccac tccccggcgt ccgcgaggtc
     4441 cttcggcggc gggctgttcc agtagcgcat gaccgccggc gcggcgaaca cggcgaacac
     4501 gtccgccgcg tccgccgcgg tcagcggccg gagcgtcagc ctgcgggtct caagagggaa
     4561 cggcgtgcta cagcgccttg cgcctgtcga ccacgcgcac ggccttgccc tcggagcgct
     4621 ggatggcctt gggttccttg agcttcaccg ccacctggat gttgagcacg ttgaagatct
     4681 gcgcctgcac gttccgctgg aagcgctcga gggcgcccat cgtctcggcg aagatgtcct
     4741 cgcccacctc gacccagacc tcgatcacgt cgaggtagcc cttgcggtcc acgacgatct
     4801 ggtagtgcgg ctcgatcgcc gggatgctca tgagcacctc ttcgatctgg ctcgggaaca
     4861 cgttgacgcc gcggatgatg agcatgtcgt cgctgcgccc gatcggcttg ctcatgcgcg
     4921 ccgaggtccg gccgcaggcg cacggctcgg cgatgagcgt gcacaggtcg cgggtgcggt
     4981 agcggatgag cggctgcgcc tggcgggtga gcgtggtgaa gacgagctcg ccgacctcgc
     5041 cgtagggcag cggccgcagc gtctccgggt cgatgatctc ggccaggtag tggtcctcgt
     5101 tgacgtgcat gccggccttg cacaggcact cgtagccgac gccggggccg cccatctcgg
     5161 agaggccgta gatgtcgagc gcgtgctcgc cgaggccggc ctcgatctgc acgcggagct
     5221 cgttggtcca gggctccgcg ccgtggaagc cgacgcgcag cggcaggtcc tccggccgca
     5281 ggccgcggtc gcgggcggcg tcggccaggt acatggcgta ggacggcgtg cagctcaggg
     5341 cggtggcgcc gaagtcgcgc atgatcttca gctgccgctc ggtgttgccg ccggagatgg
     5401 gcacgacggt gcagccaagg cgctcggcgc cgaggtgcag gccgagcccg ccggtgaaga
     5461 ggccgtagcc gaacgcgttc tgcaggaggt cgcccggcgc cacgccgccg gcggcgaggg
     5521 tccgggcgat caggtccgcc cagacgtcga ggtcgccacg cgtgtacccg acggtggtgg
     5581 ggttgccggt ggtgccggag ctggcgtgga cgcgcacgac ctggtcgcgc ggccgcgcga
     5641 acatcccgaa cgggtagtgg tcgcgcaggt cgatcttggt ggtgaagggc aggtcggcga
     5701 cgtcgccgtg gcaggtcacg tcgccgggct tgacgccgag ctcctccatc ttcttgcggt
     5761 agaacgggac gttctcgcag gcgttctcga gggtcgcccg caggcgctcc atctggagct
     5821 ccccgagctg cgggaccggc agggtctcgg cggccggctg ccagaacaga ccgccggcgg
     5881 cgtccctggt caggtgttcc tcggccacgt cgctcacagg gcccgcacct cctcgtcgct
     5941 gagcagcgtc accttgccgg cgagcagctc gacggcgcgg tcgatgtcgc gcacggcgaa
     6001 gcagatgttc cagaacgaga tgctgtacag atagtcgatg gagacgccgg cctccgccag
     6061 ctggtcgagc accccggcga gaccgccggg gacgtcctcg accttgacgc acagcacctc
     6121 cgagaagtgc gtggtgaacc cggcgtcgtg cagcagcgtg cgggcgcgct gggggtcggt
     6181 gaggatgaga cggacgatgc cgtactcggc agcctccgtc gtggagaacc cgcggatgtt
     6241 gatgcccgcc cggccgagga tgtcggcgac ttcggcgaga cgaccgctgc ggttctcgag
     6301 gaacacggtg agctgcgtga ccttcacgac tcgacctcct ggctggcgac cgcggcgcgc
     6361 ccgagctcga acgcgcgcag attgacttcg aggatcttgg cagggatcgc ggcctcgagg
     6421 ccgcgccgcc actgctcttc gccgaagggg aggaccgtgg aggcggcgcc cagcagcgcc
     6481 acgttgacgg cgcgcacggt gccggcctcc ctggcgacgc cctcggcgtc cacggtgagc
     6541 aggcgcacgc cgcgccgggc ggccgcgccg ctcacgtcgt ccgggtagcc ctcctcgcgg
     6601 cgcagcacac tgcccggcgt gatgcgcgtg gtgaaggcgc agaccagggt gcccttgggg
     6661 ccgagaaggc ccaggccgcg gatgccctca agcagctcgg tggcgatcac cacgtcggcg
     6721 cgcggcgcga ccggcgacca cacgtgcttg ccgaagcgca cggtgctgag cacgctgccg
     6781 ccgcgctgcg ccatgccctt gacctcgctg gccttgacct cgaggcccgc ggcggcggcc
     6841 gtctccgcga ggatcgccgc gccgagcagg atgccctggc cgccgacccc gacgagcgag
     6901 accgtcgtcg tcccgttcac gtctcgtccc cggcgcacgc gagactcagc gcgccgcgct
     6961 tgcacacctc gacgcacagg ccgcagccgg tgcaggtccc gagcaccgag accgcgtcgt
     7021 cgccgggggc gagtgccgga cacccgagat cgacgcagac gccgcagagg ctgcagcgct
     7081 cctcgtcgac cacgacctcg ccggccacct cacgcgtcac gagcacgcag ggcgccaggg
     7141 cgacgatcac gccgagtccc ggagccttgg cctcgtcctt gatggccgcg tcgagggcct
     7201 tgtggtcggt ggcgtcgacg gtgacgcagc gcacgccgat cccctcgcag accttctgga
     7261 tgtcgatcgt ggggctctgg gagcggtccg ggtgcaggcc cgtgccgggg tgtccctgat
     7321 gaccggtcat cgcggtggtg cggttgtcga ggacgacgac catgccctcg acctcgttgt
     7381 agaaggcgtc gacgagcggc gtgatgccgg agtggaagaa ggtggagtcg ccgatgaccc
     7441 ccacggccgc cttgcgcccg agcgccttgt tgaagccggc catcatcccg atgctcgcgc
     7501 ccatgcacag acagctgtcg agggagagca gcggcggcag cgcgccgagc gtgtagcagc
     7561 cgatgtcgcc catcggggcg aggcggcgct tgcgcagcgc agcgtacacc gagcggtgcg
     7621 agcagcctgg gcagagcacc ggcggcctga cgggcagacc ggccgccggc ttgggaggcg
     7681 tggccagcct gggcggcggc ggcatgaccg gcctccgccg ccgcggcggc ttgcgctgca
     7741 cgtagggctc cagcgcgcgg aagaccagct cctggtcgag ctcgccgtcg gccgggatgg
     7801 ccgccttgcc gatcaccggc aggccgaggg cgcgcacctg ctcctcgaag aacgggtcga
     7861 gctcctcgac gacggcgacg cgcgagacgt gctcgtacag gcgggccacc ttggcgtacg
     7921 gcagcggatg cgacatgccc agcttgagga accaggcctt ggggaacgcc tcgcgcgcat
     7981 acgcgtaggc cacgcccgag gcgatgatgc cgaggtccga cgtgcccgct tccagcacgt
     8041 tgagcgggct ctcctccgcc cgccggcgca gcttctccag gcgctcgagg accacgcggt
     8101 tccgctgccg cgcgtactgc gggaccatga cgtacttgct gatgtccctg gcgtactccc
     8161 gcggctccac ctcgaccggc acgcgcggca gcacctggcc gcgcccgtgg gagatgcgcg
     8221 tggtggtgcg caggagcacg ggcgtgtcga actcctcgga gagcttgaag gcctcggcga
     8281 cgaagtcgta ggcctcctgg ctgtccgacg gctcgagcat cgggaccttc gcgaacttcg
     8341 cgaagttgcg gttgtcctgc tcgttctggg aggagtgcat gccggggtcg tcggccgtga
     8401 tcacgacgag gccggcgttg accccggtga gcgacagggt catgaacggg tcggcggcga
     8461 cgttgaggcc gacgtgcttc atggtgacga tggtgcgcac gccggcgagc gacgccccca
     8521 ccgcggtctc gagggcgacc ttctcgttga cgcaccactc gctgtggatg ccctcgagcc
     8581 ctgcggtcgc ctcgatgatc tcggtggaag gggtgcccgg atagccgcag gcgaaccttg
     8641 ccccggcgcg gacggcgccg tgggcgatcg cttcgttccc ggagaagaag agctgacgtt
     8701 ccatgggggg gacgattcta gccgagccgg agcaccgcgc gccactccct ccggcctcgc
     8761 gcgccacggc acggagcgga tgggcgacct gcgcgcgata atctcgttcc cagtctcaac
     8821 ctgaaccctt gaccagagct tgaaggattc agcttgtcct gtcagaatac tcacggcatg
     8881 cagcgcgcaa ccttcacgcc tcgcatgact cgggcggcct cctggtgagc cgcccgtctc
     8941 gcagacgccg gcacccggca ctcctgcgag ctcgctcccg gaccctcttc ttcctgctcc
     9001 tcaccgcgct ggcgctggcg ctcggctacc gcgccgcggc ggcgccggcc gcggccggcg
     9061 cgctcctcgg cctcgtctcc atccgtgtcg cgcgcaagca ccccgccgag ctggagcaca
     9121 cgttcgtcgt gctcgactgg gcgctcctcg gcatggcgct ggtgctctcc ggcggcgccg
     9181 ggagctggct gctcgtcacc gtgccgttcc tgacgctcgg ccagctggcc gggacgtcgg
     9241 tcggggagtg gccgttcctc gcggggccgt cgctgctgct gctcgtcgtc ctcgcgatcg
     9301 ccgacccgtc gctgggcggg agccgcatcg gcgccgtcgc caagctcgcc gtgctcgtcg
     9361 ccggcggctg cgtcgcggcg acgcggctcc ggcgacgcac cgccccgcgg gcacgccggg
     9421 cgcacgcacc caaggtcgac gtcagcacgg ggctctccac gggcacgtgc ctccccgcct
     9481 tcctcgagag cgccgccgag accgcgctcg ccgagcacca gccgctgagc gtggtgtacg
     9541 tgcggctgga gcactacgag gacagccgca acttcctcgg cgccgagggc agcgaggggc
     9601 tcgtccgtgg cgtcgcgcgg cgcgccgaac gcagcctgtg cgccgacggg cgcgccttcc
     9661 gcgtgcggcc ggacggtctc gtgctggtcc tgcccggcct ctcgctcgcc gaggcgcggc
     9721 agacggcgag cgacctcgcg cgcgaggtcg gcggcagcct catcgccggg cgccggcaga
     9781 cgctggccac cggcgccgcc tcgttcccca cgctgcgcag catcgaggag ctgctcggcg
     9841 ccgcccgcga cgaggcgctc ccggaggccg tctccacaga ggtggccggc accgtcgtcc
     9901 cgctggcggc cgcacagtag acgcgggccg cgacggcccg cgtcttgcca ctccccatgc
     9961 gcctcgctag tcttcggctg tggagatcga catcaccagc cagcaggtgc cggagggcgt
    10021 ggccgtccag gtcaaggtac ggctctcggg gcgtgagacc agccggctgt tcgtgagcgg
    10081 cgacaccctc gtccagctcc cgctcgacgg cgccctgccc gacctcgacg gctctcccgt
    10141 cccccgcatg agcatctacc tcagcgagct cgccgggcgg cgggacggcc tcacccggac
    10201 cttcgccggc gaggcggccg cagaggcgtt caccgccgcc gtgcgcaccc aactcgagac
    10261 cgccttggag gcgtcatgac cgatcaggcc gacatcctcg ccgcccagct ggccccgtat
    10321 cgggagacgc ccgggatcgt ggccgccctg ctcgtctccc gcgacggctt cgtggtcgcg
    10381 gccgacgccg acgccggctt caaggccgac gccgtcgccg cccaggtggc cggcgtcatc
    10441 gacatcggcg cccggctcgc cgccgagctc ggccagccgg aggccaggta catctccgtc
    10501 gacttcgacg gcctcaacct cgtgctcgcg cccttcggcg acgagctcat gctcgtgctc
    10561 gcgggtctgc ccagcgccct gctctgcgag tatcgcctca tcaagccagg cgcctgaccg
    10621 ccctgccgga gcagccggca ggacctacca aggagacact catggaagac gcggtcgaac
    10681 aactggagaa cctggcgcgg agcgtcggcg agtgcacccg ctgcgagcag ctcgtggcga
    10741 gccggctccg cgccgtaccc gggggcggcc acccgcactg cgcggtcatg gtcgtctccc
    10801 tcgcgtcgga ccccgccgac gaggcggacg gtcgcctggc cggcgaggct ctgctcgccg
    10861 gcctcgccga attcatgccg gccctcgccg agagccgcga ccaggtgtac gtcaccgctc
    10921 tcgccaagtg cgtgccgcgc agcgacggcg ccgtgcgcgc gccggaacag caggagctcg
    10981 agaactgcta cccgtacctc tccaaggagc tcacgatcac cacgccgcac tacgtgctca
    11041 ccgtcggcga ggagaccacg cggttcctgc tcggcaagct cttcaagcac cgcccctacg
    11101 agcccggcga ctcgctcgag ctgcggctgt tcgacaaccc gtcgttcaag gtcgtgcccg
    11161 tggcggcgcc ggaggacctg cgccagagag atgccaaggc gcgcaaggag tacacggagc
    11221 gcctgcgcgc gctcgggcag gtgatgggcc tgtaggggga cccggcggcc gccggcggcc
    11281 gcggatcgga gcgctcaggg caccacgacg cgcagcgcgc gcggcacgac cctgcactcc
    11341 atcgggagct tgccggccat ttctccgtcg gtctgcacga gcacttcctg gtctccctcc
    11401 accgcctcca cgacggcgct ctcggtgcgg aagtactcga ccttggggtg cttgatgtgc
    11461 gcgttgatga gcgcagccgt ccagtaccag accgtgctga cgaacgactt gtccttgagc
    11521 acgcagacgt ccagcaggcc gtcgcgcatg tcggcgagcg gcgtgatgcc gaacgcgccg
    11581 ccgtagttgg acgagttgcc gaacacggcg aagtagccct ccacggtgcg ctccctggcg
    11641 gtcacgcgga agagcgggtc atcgtgagtg aggtaggtgc cgacgccctg gacggcgaac
    11701 gccgcctcct tgagcgcctt cttcatcgcc gggtgcatgg aggcgaccac ggcggcgtcc
    11761 atgcccacgc cggccatgag gccgaagtag cggtcgccgg cccggccgag gtcgatccag
    11821 ctgacgttgc ccgacgcgag gagcttcacc gcgtccggcg gctcgagcgg gatgccgagc
    11881 tccatggcca gcacgttcac ggtgccggtg gggatgatcg ccagcggcac gtcgctgccg
    11941 gccaggccgt tgatggtctc gttgacggtg ccgtcgccgc cgagcgcggc cacgatgtcg
    12001 accccgtcgg cgacggcctc gcgcgcgagg cgcccggcgt cgccgggctc ccgggtctcg
    12061 agtccctcca cgcgatagcc gaggccgcga agcgcgtcga agatgagcgg caggtcgcgc
    12121 ttgtacccgc cgccgcccga gtgcgggttg aagatggcgg cgagccgcct gcccgtgacg
    12181 gtcacggcag cggctcctgc tcgcccgacg cgatggagcg gagcgccgtg tagccggcgg
    12241 cgcgggcgag cccggcggcg tggtcgaagc cgtggccgac gtcgccgggc cggtgcgcgt
    12301 ccgagccgag cacgagcggg atgccgaggc cgccggcctc ggccagcagc gccggcgccg
    12361 ggaacgcccg gccgatgggc ttgcgcaggc cgccggtgtt gatctcgagg gccatgccgg
    12421 ccgccgccac ggcgcgcagc gccgcggacg cggcggccgc gaccgcctcc gcgtcctccg
    12481 gcggcccgaa cttggcggcg tggtcgaggt ggccggccac gtcgaacagc cccgactcgg
    12541 ccgcctgccg cgtcaggtcg aggctctcga ggtggatgcg gccgatcccg taggcgccga
    12601 ggcgcccctc catctcggga tggtcgaagc cgaagcggtc gccgacgatg tgcaccgacc
    12661 cgatgacgta ctcgaacggg tactgcgcga gcaccgccgc cgtgtcctcc atggtctcgg
    12721 ggatgaagtc ggcctcgatg ccgagcacga tgcgcaggtc ctgagcgtac tcgcgggcga
    12781 gatcctggac gagcgtgacg tagtcgtcca gctcgtccag aggcatggcc cagtcgtcgg
    12841 tgaggtcgtg ccgaggttcc cagccgccga ggaacggcag gtggtcggag aaccccatcg
    12901 cggtcatccc gagctccagg gcgcgctcga cgtactcccg cgcctcacct tccgcgtggc
    12961 ggcacatgcc ggtgtggatg tgatagtcgg cgatcatgag agaccggggg acgcgacggc
    13021 ccgcccgtgc cggcaagctt acacccgccg ccgcgccccg gtggcccggc gcctccgggc
    13081 gaacagcagc acgagcagca tcccggcgac gaacccaccg aggtgggcga agaacgcgac
    13141 gccggcctcc cccgacccgt aggactgggc cccgcccacc ccctgcagca cgaaccagac
    13201 cacgagcagg atccaggccg gcacctcgaa caccggccag acgatgatga gcgggatcac
    13261 ggtgagcacc cgggcgcgcg ggaagagcac gaggtacgcg ccgagcacgc cggcgatcgc
    13321 gccgctggcg ccgatgagcg gcacgtcgct cgtgggggcg accagcacct gcgccgtggt
    13381 cgccgccatc ccgcagacga agtagaagac gacgtaccgc ggccgcgtca tcgcgtcctc
    13441 gacgttgttg ccgaagatcc acaggtacca gaggttgaag atgaggtgca gccagccgcc
    13501 gtgcacgaac agcgacgtga acagagtggc ccaccagggg aaccagccga agggcgggat
    13561 gtccacgcgg tgcgtgagct cgaagggccg cgcgcccagg aggtagtacc agccctcgag
    13621 cgtcacgccc cagcggggca ggaagagctg cacgacgaag accgccgcgt tggcggcgat
    13681 gagcgccgcc gtcacggccg ggaagcggcg caccggattg tcgtcgtgca gcgggatcac
    13741 aggggcgagt gtaccggccc ggccccgcgc atccagcagc cgagccgggc cgcggtggcc
    13801 gttgtcgccc atccggacaa cgccggcgca cggcgcgtca ggctcgggcg aggagaacga
    13861 cccccacacg gcgaatgaca ccgcatctca cgtggtaccg tctgtagtgt cggaggaggt
    13921 cgtgcatgcc cgggacgacg cagagcgccg agccgcgagt catcgtgatc ccgcgtcagc
    13981 tcgaggggtt cttctacgag cgcctgacgg cggagttcgc cgcccgcgac gacgtccgcg
    14041 tggtcgtgga ccggcgcgtc ggcgagcgcc ggcgcccccg ctggggcagc ggcgccggcc
    14101 ccttcaccga gcgcaggggc ggcgaccgtc gcgcccagag cccggcgtgg tccctgccgg
    14161 acatgccgtt ctccgcctcc tgacgcgcga gaagaccgta cgcgagaagg gccggcgtct
    14221 gccggccctc ttcacgttcg gtctcgtctg agcgaggctc agatccagcc gttctcccgg
    14281 ctcacgatga ccgcctgggc gcggtcgacc acgttcagct tgtggtagac gttgtgcagg
    14341 tggttgcgga ccgtgctctg gctcagttcg agcgcccgcg cgacctgctt gtacgtcttg
    14401 ccgctcgcca gcaggccgag cacctcgagc tcgcggtccg tcatcgggtt gggcacgtcg
    14461 cccctgggcg ggccgagctc acccaggtgc accttgacgc gcgccagcag ctccttgatg
    14521 tggaacggct tggtgatgta gtcggcggcg cccttctcga agccgagcag cttgtcgttg
    14581 atctcgcggc gcgccgtgag gaagatgacc ggcgagcgca gttcgcaggg cttctcgcgc
    14641 agtctctcgt acaccgcgaa cccgtccatg tccggcatca tgatgtcgag gagcacgagg
    14701 tcggggaggt agaggcccgc ggtgcgcagc gcgtcgccgc cgttcgcggc cgtgtgcacg
    14761 tcgtacccgg tcacttcgag cgcctcggcg acgagccgcc tgatgtcggg ctcgtcgtcg
    14821 acgacgagga tccgctttgc catcgctcac tctcctgccg ccggggtcgg gggccgcgcc
    14881 ggcgttccca gtatcggcaa caccggggcg aaccttaggg gcaggcgccg gggatcagcg
    14941 gatgaggcgg tcgccgaagg ccaccagcag cacggtgacc gcgaccacca cggcgacgcc
    15001 gatggcgatg tccccgtagc ggcgcgcgcg cacggcgtgg gtggtcagcg tcaccaggcc
    15061 gtagccggcg accaggagac ccgcgacgcc ggcgatcagg atcacccagt tcacgaccgc
    15121 catcgtacac ggtcggcgga ggcgatcgtc agggctgcag gcgggcggcg aggaactcga
    15181 gcgggtgcag ggcggtccgg ccggcgagat cgccgatctg cgtgcggcac gaggtgccgg
    15241 tcgccaggac cacggtctcc gggctcgccg cccgcaccgc ggggagcagc gcgcgctcgg
    15301 ccatggccac gctgagctcg tagtgccggg cctcgtaccc gaagacgccg gacatgccgc
    15361 agcagccggc gtcggggtcc tccacctcga ggccgggcac ggcggccagc gcggcggcgg
    15421 tcgcggcggc aaagccgagc gccttctcgt gacagtggct gtggaggagg gcgcggccgc
    15481 cggcacggaa gcgcaggcgg ccttcggcgg ccaggccggc gacgagctcg agcgccggcc
    15541 tgctcgcggc cgccacctcc gccacgcggt ggtcgtgcgg cagaaggcgc gcccagtcgt
    15601 cgcgcaccat cgagaggcac gacggctcga cgaagaggat ctcggccccg gcggacgcca
    15661 gcgggtacag gccgtcgagc gcccgcagcg cgtgcccgcg agccttctcg atctgcccgg
    15721 tcgagagcgc ggtacggccg cagcagcccg cgtcggccgc ggccagcggc aggccggcgg
    15781 cggcgagcag gcgggcgagc gcctcgccga tgcgcggctc ctggtactgg atgaagcaat
    15841 cgacgaacag cgcggcggcc gggcgggcgg cggcgtgctc cccctgcggc ccgggcaccg
    15901 gcgacaggcg gcgcgtgagc ggccgccggg cgaaccgcgg cagcggccgc tccgcggcca
    15961 cgccggcctg ccgcgccagc gcgcgcgcca ggcgggtgcg gccgagcccg ttcaccagcg
    16021 gcgcgaccgg cgcggcgagg gcggcgagcc g
//
LOCUS       DASUMX010000264         3684 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_383270, whole genome shotgun sequence.
ACCESSION   DASUMX010000264 DASUMX010000000
VERSION     DASUMX010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3684
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383270"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..776)
                     /locus_tag="VFZ86_12630"
     CDS             complement(<1..776)
                     /locus_tag="VFZ86_12630"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEX5643162.1"
                     /translation="MRPRLDRLRALLDGLRVDGVVVTDPVDVRYLSGFRGEDATLVVG
                     HDAALLCTDARFWEQVREEVDGFELSKVTAEPLLAHSVHEAARVLGAGARVGYQGAQL
                     SHASYRRLRRLHAGPLRDVRQGVTLLRAVKDAAEIETMRRAAAVTDEALGLVVARGLV
                     GRKERDVAWDLQAEYHRLGAEGEAFGAIVAAGDHGAQAHALPGERVIRAGELVVIDTG
                     ARVDGYCSDITRTFAAGEPAGELRRMYDAVLAAQLAGLAAV"
     gene            complement(773..1240)
                     /locus_tag="VFZ86_12635"
     CDS             complement(773..1240)
                     /locus_tag="VFZ86_12635"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020875883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEX5643163.1"
                     /translation="MAAKALKNLWLLHGVNLDMLGRRDPSVYGSMTLTELEAYVARHG
                     EALGFTVRSFHTNHEGEFVEKLHQLTNDGADAAIINPGAWTHYSYALHDALELVEAPV
                     AEVHLSDIEDREEWRRVSVIADVVDVRVRGKGAAGYVEALRALAQLVEGRAQG"
     gene            complement(1222..2307)
                     /gene="aroB"
                     /locus_tag="VFZ86_12640"
     CDS             complement(1222..2307)
                     /gene="aroB"
                     /locus_tag="VFZ86_12640"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEX5643164.1"
                     /translation="MKRLTVHAASRTYPILVGRGVLDELGAPVRRLRGEGQVVVVSDE
                     NVAPLYLDRVRASLTAAGLSTSQVILPAGEREKSLARAEELYGVLYDRGLRRSDTLVA
                     LGGGVIGDLTGFVAATYQRGIGLVQVPTTLLAQVDASVGGKVAVDFRAGKNYVGTFYQ
                     PSLVLADLDTLHTLPARELRGGAAEVAKHGLLAGSAVLRRVRQLARGPLAAEAVTQEL
                     VAGSIQYKAGVVERDELEAGLRAVLNFGHTIGHAIEAATGFARFSHGEAVALGLHAAL
                     RISGELARLPEAEELAAHELLDALGLPARLTGVRAQDVCDLISRDKKAGQDGVGYVLL
                     RRPGFPVIGVRVSPELELEVVEWLRKR"
     gene            complement(2304..2882)
                     /locus_tag="VFZ86_12645"
     CDS             complement(2304..2882)
                     /locus_tag="VFZ86_12645"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEX5643165.1"
                     /translation="MPETCDHPPAVALVGFMGAGKSAVGRELAAALGSAFVDTDELIA
                     AQAGPIETIFAERGEAAFRALEAEVVTAAVREARERPRVLALGGGAVLSAAVREALGR
                     LPHVVWLTAPPEVLWRRARTEGQAVRPLALDETAFRALLATRESLYRQVATVVVDTAA
                     KPVAAIAADIAGRLHEAPPEAAARRRAQGGVR"
     gene            complement(2890..>3684)
                     /gene="aroC"
                     /locus_tag="VFZ86_12650"
     CDS             complement(2890..>3684)
                     /gene="aroC"
                     /locus_tag="VFZ86_12650"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEX5643166.1"
                     /translation="DDIRDVIERASARETAAKVACGAVAKRLLAALDCTIASHVVAIG
                     AVEAERGMSLDDVTRVEASPVRCRDEEAGRRMIAAIDAAAEAGDALGGVVEVLAFGFP
                     SGVGSYVQGDQRLRSRLAGAVLSVPAIVGVEFGLGFAAAALPGSRVHDPIAHDTGRGY
                     VRASNGSGGIEGGISTGGTVVLGAAMKPISTLREPLDSVELGTHRAVKSRFERSDVCA
                     VPAVGIVLEAVLALALADAALERFGGATVGDLVAAVAAYQRRIRER"
BASE COUNT          492 a         1423 c         1311 g          458 t
ORIGIN      
        1 acggcggcga ggccggcgag ctgcgccgcg aggacggcgt cgtacatgcg gcgcagctcg
       61 ccggcgggct cgccggcggc gaaggtgcgc gtgatgtcgc tgcagtagcc gtcgacgcgc
      121 gcgccggtgt cgatcaccac cagctcgccg gcgcggatga cgcgttcacc cgggagggcg
      181 tgcgcctggg cgccgtggtc gccggcggcg acgatcgcgc cgaacgcctc gccctccgcg
      241 cccaggcggt ggtactccgc ctggaggtcc caggccacgt cgcgttcctt gcggccgacg
      301 aggccgcgcg caaccaccag gccaagggct tcgtccgtca ccgcggccgc gcgacgcatg
      361 gtctcgatct cggcggcgtc cttgaccgcg cgcagcagcg tgacgccctg gcgcacgtcg
      421 cgcagcggtc cggcgtggag gcggcgcagc cggcggtagg aggcgtgact gagctgcgcc
      481 ccctggtacc cgacgcgcgc gccggcgccg aggacgcgcg cggcctcatg cacgctgtgc
      541 gcgagcagcg gctccgccgt caccttgctc agctcgaagc catcgacctc ctcgcgcacc
      601 tgctcccaga accgcgcgtc ggtgcagagc agcgccgcgt cgtgcccgac gacaagcgtc
      661 gcgtcctcgc cgcggaaccc cgacaggtag cgcacgtcga cgggatccgt gacgaccacc
      721 ccgtcgacgc ggagcccgtc gaggagggcg cgcaaccggt cgagccgcgg cctcatccct
      781 gcgccctgcc ttccacgagc tgggccagcg ccctcagggc ctcgacgtag cccgccgcgc
      841 ccttgccgcg cacgcgcacg tcgacgacgt cggcgatcac cgacacgcgc cgccactcct
      901 cccggtcctc gatgtcggag aggtgcacct cggcgaccgg cgcctcgacc agctcgagcg
      961 cgtcgtgcag ggcgtagctg tagtgcgtcc aggcgccggg gttgatgatg gcggcgtccg
     1021 caccgtcgtt cgtcagctgg tggagcttct cgacgaactc gccctcgtgg ttggtgtgga
     1081 agctgcgcac cgtgaacccg agcgcctcgc cgtgccgcgc cacgtaggcc tcgagctccg
     1141 tcagcgtcat gctgccgtac accgagggat cgcgccggcc gagcatgtcc aggttgaccc
     1201 cgtgcaggag ccagaggttc ttcagcgctt tcgcagccat tccaccacct ccagttcgag
     1261 ctcaggcgag acgcgcacgc cgatgacggg gaagccggga cggcgcagga ggacgtaccc
     1321 cacgccgtcc tgcccggcct tcttgtcgcg gctgatgagg tcgcagacgt cctgcgcgcg
     1381 cacgccggtg agacgggcgg gcaggccgag ggcgtcgagc aactcgtggg ccgccagctc
     1441 ctccgcctcg ggcaggcggg ccagctcgcc cgagatccgc agcgccgcat gcagcccgag
     1501 agcgaccgcc tcgccgtgag agaaacgcgc gaagcccgtc gcggcctcga tcgcgtgacc
     1561 gatggtgtgg ccgaagttga gcacggcgcg caggccggcc tcgagctcgt cgcgttccac
     1621 gacgccggcc ttgtactgga tggacccggc caccagctcc tgcgtcacgg cctccgccgc
     1681 gagcggcccc cgcgcgagct gccgcacgcg gcgcagcacc gcgctccccg caagcaggcc
     1741 gtgcttggcc acctcggcgg cgccgccgcg cagctcgcgc gcaggcagcg tgtgcagcgt
     1801 gtccagatcg gccaggacga ggctcggctg gtagaaggtg cccacgtagt tcttgccggc
     1861 gcggaagtcc acggcgacct tgccgccgac ggaggcgtcc acctgcgcca gcagcgtcgt
     1921 gggcacctgt accagcccga tgccacgctg ataggtggcg gcgacgaacc cggtgaggtc
     1981 gccgatcacg ccgccgccca gcgccaccag ggtgtcgctg cggcgcaggc cgcggtcgta
     2041 cagcaccccg tagagctcct cggcgcgggc gaggctcttc tcccgctcgc ccgccggcag
     2101 gatcacctgc gacgtgctga gtcccgcggc cgtcaggctc gcgcggaccc tgtccaggta
     2161 cagcggcgcc acgttctcgt cgctgaccac gacgacctgc ccttcgccgc gcagcctgcg
     2221 caccggcgcg ccgagctcgt cgagcacgcc gcggcccacg aggatggggt acgtacgcga
     2281 cgcggcgtgt accgtgaggc gcttcacctg acgccgcctt gcgcacgcct gcgggcggcg
     2341 gcctcgggcg gcgcctcgtg caggcgtccc gcgatatcag ccgcgatggc agcgacgggc
     2401 ttcgcggcgg tgtcgacgac gaccgtcgcg acctgccggt acagcgactc acgggtcgcg
     2461 agcagcgcgc ggaaggcggt ctcgtccagg gcgagcggac gcaccgcttg cccctccgtc
     2521 cgcgcgcgcc gccacagcac ctcgggcggc gcggtgagcc agacgacgtg tggcaagcgg
     2581 ccgagggcct cgcgcacggc cgcgctcagg accgcgcccc cgccgagcgc gagcacacgc
     2641 ggccgctccc gggcttccct cacggctgcc gtgacgacct cggcctcgag cgcgcggaac
     2701 gcggcctcgc cgcgctccgc gaagatcgtc tcgatcggac ctgcctgcgc cgcgatgagc
     2761 tcgtcggtgt cgacgaacgc ggaacccagg gccgccgcca gctcccggcc gacggcgctc
     2821 ttgccggcgc ccatgaagcc gacgagggcg acggccggcg ggtggtcgca ggtctcgggc
     2881 accgccggct cagcgctccc ggatgcggcg ctggtaggcc gccacggccg cgacgaggtc
     2941 gccaacggtc gcgccgccga agcgctccag cgcggcatcg gccagggcca gggcgagcac
     3001 cgcctcgagg acgatcccga ccgccggcac ggcacagacg tcggagcgct cgaaccgcga
     3061 cttgacggcg cggtgcgtcc ccaactccac gctgtccagc ggctcccgca gggtggagat
     3121 gggcttcatc gcggcgccca ggacgaccgt ccctccggtg gagatgccgc cctcgatacc
     3181 gccggaaccg ttgctcgcac gcacgtaccc gcggccagtg tcgtgcgcga tcggatcgtg
     3241 cacccggctt ccgggaagcg ccgcggcggc gaacccgagg ccgaactcca cgcccacgat
     3301 ggccggcacg ctcaggaccg cccccgcaag cctcgagcgc aggcgctggt cgccttgcac
     3361 gtagctgccg accccagacg ggaacccgaa ggcgagtacc tcgacgaccc cgccgagcgc
     3421 gtcgccggcc tccgcggcgg cgtcgatggc ggcgatcata cgccgcccag cctcctcgtc
     3481 gcggcagcgc accgggctcg cctccacgcg cgtgacgtcg tcgaggctca tcccgcgctc
     3541 cgcctcgacc gcgccgatgg cgaccacgtg gctggcgatc gtgcagtcga gggcggcgag
     3601 caggcgcttc gcgaccgcgc cgcaggcgac cttggctgca gtctcgcgcg cactcgcgcg
     3661 ctcgatgacg tcgcggatgt cgtc
//
LOCUS       DASUMX010000265         4736 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1391123, whole genome shotgun sequence.
ACCESSION   DASUMX010000265 DASUMX010000000
VERSION     DASUMX010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4736
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1391123"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="VFZ86_12655"
     CDS             complement(<1..483)
                     /locus_tag="VFZ86_12655"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEX5643167.1"
                     /translation="MSRLRYLTAGESHGPGLTAVLDGCPAGLVVDVGAVDAELARRQS
                     GYGRGERQSIEQDRVRVLGGIRHGRTTGAPLALFIPNRDAANWGDVLAVEAPEEGHDA
                     PELVVVPRPGHADLGGALLYGLDDIRDVIERASARETAAKVACGAVAKRLLAALGCTV
                     A"
     gene            complement(613..1443)
                     /locus_tag="VFZ86_12660"
     CDS             complement(613..1443)
                     /locus_tag="VFZ86_12660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643168.1"
                     /translation="MSQNRRLLTYVAIALIVGAALALAVALASSGADTSGVAASSGTT
                     ADSALYSDDAGTTALAAADPAKEQPLLDKFTSKDPFIPFPTPGASATGTPSPNPTSTS
                     EPTDLSAKIKVDGTSYSVVPGDQVPGGSAAAFKVTGVTSGDVTFQVLEGELENGETSF
                     SVNVGEAVRVTFVDSGVSYDISVISVGSASTGGGSGGSSDDASHSITVLSVSSQNGVA
                     MATFEVDGKTYADKKVGDVFTTSWGEIEVLSINVGAQTVTILHGDQTLTLHAGQVIVK
                     "
     gene            complement(1440..2075)
                     /gene="pilO"
                     /locus_tag="VFZ86_12665"
     CDS             complement(1440..2075)
                     /gene="pilO"
                     /locus_tag="VFZ86_12665"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HEX5643169.1"
                     /translation="MRARGKEIYIITAIVAVVLIVAWYFLLFSPKQRELDDLDQQVQS
                     AQTALAEAQNTVVQLEAYKKTAPQSRAEVVRLGKMLPTSEGIPGLIIELSKTAEASGV
                     SVTSIARGTTTPGTPYGIQALTLQVSGRYFDVEDFLYRLEEYVAFRNASFRVTGRLLQ
                     VTQLTLQAATTSESTGGSPPLAVTIGMNAYLWGGVAQTAPAVEDATAGGAQ"
     gene            complement(2072..2728)
                     /locus_tag="VFZ86_12670"
     CDS             complement(2072..2728)
                     /locus_tag="VFZ86_12670"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEX5643170.1"
                     /translation="MKRINLLPQEERAKASRERGLIWAILILVAVVVVLGLVYVKLNS
                     DANAKQDELAAIQAETAVVQAKIAELAPYAELQAQRTSMTETAKGIEESSVPFSTLLQ
                     ELSLVIPENVRLQTLTATVPTTMLPGTALAEAAPGAATSTDVTFTGQTEKHRDVAEFM
                     TRLGLIPQLMNITLTTSTDSSTASTTTTTTFKQFTVTAQLRPYTTQPPTTTLQTEVAP
                     "
     gene            complement(2734..3831)
                     /gene="pilM"
                     /locus_tag="VFZ86_12675"
     CDS             complement(2734..3831)
                     /gene="pilM"
                     /locus_tag="VFZ86_12675"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HEX5643171.1"
                     /translation="MNAVRVGIDIEQASMVGAQIRTGRQGHTLMAVSARALPDGLMFE
                     GEVVDIDGLAAELKTFWKAAGFSGKRFSVGIANQKIVVRTMEFPIIDPKELRAAIEFQ
                     AQESIPIPLDEAILDFEVLSTTPGEGEGGGRQQVLIVAAQRDMVNQFMQVAKKAGLAI
                     EGIDLQAFALMRSVAPPVAFVDQGAPQADGASALINIGAGISNIVVASGGNPQFTRVV
                     NLGYETLVQAVMQNRGITHQEADLLRLTVGLTGSAASAADLEPATVGEVHGVFDSTCE
                     SFADEIRRSIDYYHSLQQDAPIARILISGEGALTRNMCDYLAGALHLPVALGNPLQFI
                     AENKSKLPQQELELIAPRLAIALGLAFEDEE"
     gene            complement(4020..>4736)
                     /locus_tag="VFZ86_12680"
     CDS             complement(4020..>4736)
                     /locus_tag="VFZ86_12680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643172.1"
                     /translation="APLIVGGSGVYVGGCLRGRENVSFADAADAVPGLVAGSPADVVQ
                     GEAYPAAAVHSGVGIFALGTEIHEVADGAASYAQDTDRHTGLPVPDDWLAGPSAEFLL
                     AASAVAGSAGPAFTGAGLDLEQIGAATGDDAITGRCILLPRLDEVTLSGSLDLAAGRL
                     LIIVPGDATVGSPGESTVLSGALVVCGHLRVRGELLLDGSLHAGSIAIDAPTTVSVRS
                     DWRERPLAGAVVMTIVEHGQ"
BASE COUNT          685 a         1642 c         1633 g          776 t
ORIGIN      
        1 ggcgaccgtg cagccgaggg cggcgagcag gcgcttcgcg accgcgccac aggcgacctt
       61 ggcggcggtc tcgcgcgcgc tcgcgcgctc gatgacgtcg cgaatgtcgt cgaggccata
      121 cagcagggcg ccgccgaggt cggcgtgacc cggcctcggg acgacgacca gctccggtgc
      181 gtcgtgtccc tcctccggcg cctccaccgc cagcacgtcg ccccagttgg cggcgtcgcg
      241 attggggatg aagagcgcca gcggggcgcc cgtcgtgcgg ccgtgccgga tgccgccgag
      301 cacgcgcacc cggtcctgct cgatgctctg ccgctcgccg cggccgtacc cgctctggcg
      361 ccgggcgagc tcggcgtcga cggcgccgac gtccacgacc agccccgccg gacagccgtc
      421 cagcacggcg gtcagcccgg gcccgtggga ctccccggcg gtcagatagc gcagtctgct
      481 catggtccta ccttatccgc gccgcgctca caggcacaag gcgagcccgg cccgccgatc
      541 cggcgacgta cgcgcgacgg ggcggcctgc ggccgccccg tcgcctcact gacccgctgc
      601 cggcccgccg ggctatttca cgatcacctg gccggcgtgc agggtgagtg tctggtcgcc
      661 gtgcaggatg gtcaccgtct gggcgccgac gttgatggac agcacctcga tctcgcccca
      721 ggaggtggtg aacacgtcgc ccaccttctt gtcggcgtag gtctttccgt ccacctcgaa
      781 ggtcgccatg gcgaccccgt tctggctgct gacgctgagc acggtgatgc tgtggctcgc
      841 gtcgtcgctg ctcccgccgc tgccgccgcc ggtgctggcg gagccgacgg agatgaccga
      901 gatgtcgtag ctcacgcccg agtcgacgaa ggtcacgcgc acggcctcgc ccacgttcac
      961 ggagaagctc gtctcgccgt tctcgagctc gccttcgagg acctggaagg tgacgtcgcc
     1021 tgaggtcacg ccggtcacct tgaacgccgc cgcggagccg cccggcacct ggtcgcccgg
     1081 caccacgctg taggacgtcc cgtcgacctt gatcttcgcc gacaggtccg tgggttcaga
     1141 cgtactcgtc ggattggggc tcggcgtacc tgtggctgag gcccccggcg tcgggaacgg
     1201 gatgaacggg tccttgctcg tgaacttgtc gagcagaggc tgctccttgg ccggatcggc
     1261 cgcggcgagc gccgtcgtgc cggcgtcgtc ggagtagagc gcggagtccg ccgtcgtgcc
     1321 gctcgaggcg gcgacgccgg aggtgtccgc gccgctgctg gcgagcgcga ccgcgagcgc
     1381 cagagcagcg ccgacgatga gcgcgatggc gacgtaggtg agcaggcgtc gattctggct
     1441 cattgcgcac ctccagccgt cgcatcttca acagccggcg cggtctgcgc caccccgccc
     1501 cagagatagg cgttcatgcc gatcgtgacc gccagcggcg gcgagccgcc ggtgctctcg
     1561 ctggtggtcg ccgcctgcag ggtgagctgc gtcacctgca gcaggcgtcc ggtcacgcgg
     1621 aagctcgcgt tgcggaaggc gacgtactcc tcgaggcggt agaggaagtc ctcgacgtcg
     1681 aagtagcggc cgctgacctg cagcgtgagc gcctggatac catacggggt ccccggcgtc
     1741 gtcgtgccgc gggcgatgct ggtcaccgag acgccggatg cctccgcggt cttgctgagc
     1801 tcgatgatga gcccggggat cccttcgctc gtcggcagca tcttgccgag ccgcacgacc
     1861 tcggcgcgtg actgcggcgc cgtcttcttg taggcctcga gttggaccac ggtgttctgg
     1921 gcctcggcca gcgccgtctg cgcggactgc acctgctggt ccaggtcgtc cagctcgcgc
     1981 tgcttggggc tgaacaggag gaagtaccag gcgacgatca gcacgaccgc cacgatcgcg
     2041 gtgatgatgt agatctcctt gccgcgtgcc ctcatggcgc cacctccgtc tgcagcgtgg
     2101 tggtaggcgg ctgcgtcgtg tacggccgca gctgcgccgt caccgtgaac tgcttgaagg
     2161 tggtcgtggt cgtcgtggac gcggtgctgg agtccgtcga cgtcgtgagc gtgatgttca
     2221 tgagctgcgg gatgaggccg agccgcgtca tgaactcggc cacgtcgcgg tgcttctccg
     2281 tctggcccgt gaaagtgacg tccgtgcttg tagcagcgcc cggcgccgcc tcagccaagg
     2341 ccgtccccgg cagcatggtc gtcgggacgg tcgcggtgag cgtctgcagg cggacgttct
     2401 ccgggatcac caggctgagc tcctgcagga gggtcgagaa cggcacactg gactcctcga
     2461 tgcccttcgc cgtctccgtc atcgacgtgc gctgcgcctg cagttctgcg tatggagcga
     2521 gctcggcgat cttggcctgg acgacggcgg tctccgcctg gatggcagcg agctcgtcct
     2581 gcttggcgtt ggcgtcgctg ttgagcttga cgtacacgag accgagcacg acgacgaccg
     2641 cgaccaggat gaggatcgcc cagatgagac ctcgctcgcg cgaggccttg gcgcgttcct
     2701 cctggggcag caggttgatg cgcttcatgg tgcctactcc tcgtcttcga aggccaggcc
     2761 gagggcgatc gcgagacgag gggcgatcag ctcgagctcc tgctgcggaa gcttggactt
     2821 gttctcggcg atgaactgaa gtggattgcc gagcgctacg ggaaggtgca gagcgcccgc
     2881 gagatagtcg cacatgttgc gggtgagcgc tccctcaccg ctgatcagga tgcgcgcgat
     2941 gggcgcgtcc tgctggagcg agtggtagta gtcgatcgag cgacggatct cgtcggcgaa
     3001 cgactcgcag gtggagtcga acaccccgtg tacctcgccc accgtcgccg gctccaggtc
     3061 ggccgccgac gcggcgctgc ccgtgaggcc gacggtgagg cggagcaggt ccgcctcctg
     3121 atgcgtgatg ccgcggttct gcatgacggc ctgcacgaga gtctcatagc cgaggttgac
     3181 gacgcgcgtg aactgcggat tgccacccga cgccaccacg atgttgctga tccccgcgcc
     3241 gatgttgatg agcgcgctgg cgccgtccgc ctggggcgct ccctggtcga cgaaggcgac
     3301 cggcggagcc acggagcgca tgagcgcaaa ggcctgcagg tcgatgcctt cgatcgcgag
     3361 gccggccttc ttcgccacct gcatgaactg gttgaccatg tcgcgctggg cggcgacgat
     3421 cagcacctgc tggcggccgc cgccttctcc ctcgccgggc gtcgtcgaga ggacctcgaa
     3481 gtcgaggatc gcctcgtcga gcgggatcgg gatggactcc tgcgcctgga actcgatcgc
     3541 ggcgcgcagt tcctttggat cgatgatggg gaactccatc gtgcggacga cgatcttctg
     3601 gttggcgatg cccacggaga accgcttgcc ggagaagccg gccgccttcc agaaggtctt
     3661 gagctccgcc gcgaggccgt cgatgtcgac gacctcaccc tcgaacatga ggccgtcggg
     3721 cagcgcccga gcggacaccg ccatcagcgt gtgcccctgg cggccggtcc tgatctgggc
     3781 accgaccatc gaggcctgct cgatatcgat gcctacgcgc accgcgttca tggccatatt
     3841 cgcccctcag cttgccttgc cctagcgcca gtgtcggcac ccgcgcggga tacctttagg
     3901 cactcgttcg acgggttgac gctcgccccc tgccctccac gtcaaggaag gctactcttt
     3961 cacagactgc gtgccgcgta cagagggttc gcggcacacg tgactcatcc tgcccgcctt
     4021 cactgaccgt gctccacgat cgtcatcacc accgcccccg ccagcggtcg ctcgcgccag
     4081 tccgagcgca ccgagaccgt agtcggagcg tcgatggcga tgctccctgc gtgcagactg
     4141 ccatccagga gcagctcccc gcgcacgcgc agatgcccac acaccacgag ggctcccgag
     4201 agcaccgtcg actccccggg agagcctacg gtggcgtcgc cgggcacgat gatgagcagc
     4261 cggccagcgg caaggtccag agagccgctg agcgtcacct catccagccg cggcagaagg
     4321 atacagcgac ccgtgatcgc gtcatcgcca gtggccgcgc cgatctgctc caggtcgagg
     4381 ccggcgccgg tgaaggcagg accggccgat ccggccacgg ccgacgccgc cagaaggaac
     4441 tcggcggagg gccctgcgag ccaatcatcc gggacgggca gccctgtatg acggtcggtg
     4501 tcctgagcgt aggatgctgc gccgtcagcc acttcgtgga tctccgtgcc gagcgcgaag
     4561 atgcccacgc cgctgtgcac tgccgcggcg gggtacgcct ccccctggac gacgtccgcg
     4621 gggcttccgg cgacgagacc cgggaccgcg tcagcggcgt ccgcgaagct gacgttctcc
     4681 cgccccctca gacagcctcc aacgtacacg ccgctcccgc cgacgatgag cggcgc
//
LOCUS       DASUMX010000266         6478 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1678637, whole genome shotgun sequence.
ACCESSION   DASUMX010000266 DASUMX010000000
VERSION     DASUMX010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 6478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6478
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1678637"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..929)
                     /locus_tag="VFZ86_12685"
     CDS             complement(90..929)
                     /locus_tag="VFZ86_12685"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX5643173.1"
                     /translation="MLAVVLALAASLSYGTGDFLGGSQTRRTSLWTVIVVSQPTGLLL
                     MALIVLVRGEPLPQDVLLPAFAAGLLMVVTLAAYYQALAVGVMSIIGPIFSLSVAVPV
                     IVGLARGERPSALQLAGIAIALGGIFLASREKSAGEHHTATSRVSIGLALLSGTAYGF
                     VMVFYAQGAEADPYWSIALSRLTSATVFALAFLVVRPALRYTRGAAAPLLAIGVFEVG
                     AFTLFSVASTLTYLSIVSVLGSVYPVFLALLAYAFLRERLARAQLVGVAATLAGVALI
                     AAG"
     gene            complement(960..1598)
                     /locus_tag="VFZ86_12690"
     CDS             complement(960..1598)
                     /locus_tag="VFZ86_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010644553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5643174.1"
                     /translation="MTEPGEGQQRRGREATTTAILDAAEELFSERGYAAVSVREVAAR
                     AGVSHALVHRYIGSKAELFRAVLTQRENAILAAAPDNPDLLQSTSLMLRRGLEYGRSH
                     IRLIVRSAMSDLPYDRTTGRFEATERLIALAERAAASAPPAERAEKDLDPRIAVACVV
                     ALFLGWGTTDSWLRPAAGLADMADAELLDGIERVILDILRVEVPGVEADPPV"
     gene            complement(1582..2427)
                     /locus_tag="VFZ86_12695"
     CDS             complement(1582..2427)
                     /locus_tag="VFZ86_12695"
                     /inference="COORDINATES: protein motif:HMM:NF012730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HEX5643175.1"
                     /translation="MKPRFSNSYELFILLLTVLSLLAMALLLLPWLAPATVQLLQIYD
                     FVMCLVFLLDFGLRMKRASSWRTYFFRERGWLDLLGSIPSLGISRYGALLRLARLSRL
                     VRVLRLFRSQGQSGLAKDVLANRGQYAGFITLISAMTVLSVSSVVMVQSESKVAEANI
                     ATGGDALWWALVTITTVGYGDQYPVGAVGRVIGAVVMIAGVGIIGALASIFASVLIPP
                     PEKQVDPAAELGELKTQLAVMHEELTAIRSLLSVEGVLAGCEEHSDPATTARRRGGSR
                     DDRAR"
     gene            complement(2609..2920)
                     /locus_tag="VFZ86_12700"
     CDS             complement(2609..2920)
                     /locus_tag="VFZ86_12700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643176.1"
                     /translation="MYVLNYWHTVSDYAEWKKIFDSDPLGREESGVRRVSIERPVDDE
                     NTVIGELEFDSLGEAETFAGRLEEIWKGSASRTVSNAGLRITEVLEQQQFGSESSRRA
                     A"
     gene            3197..3931
                     /locus_tag="VFZ86_12705"
     CDS             3197..3931
                     /locus_tag="VFZ86_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HEX5643177.1"
                     /translation="MDAVIAREKHRRTAIAVGALFIVGDIAGVLSVLVTRGLLEGPDV
                     LTRIAADQGRLALGALLVLVMGFALAMVPLLMYPVFKKYNEVLALGTVVFRSALETAG
                     YMATAGAMLLLLGLSREPVEAASTGASPFEALGAFLVSAQGSTTAYLTAIAFALGSLM
                     FYALFYQSRLVPRWLSVWGLAGAVLYLAAPLLDMFGHGFGILMAPLAVAEIVLAVWLI
                     AKGLDAPAPARTPAPQRTPVLDRTPA"
     gene            3979..4911
                     /locus_tag="VFZ86_12710"
     CDS             3979..4911
                     /locus_tag="VFZ86_12710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643178.1"
                     /translation="MSTSPRHVVEAQRSRPPGGRAAPWIALALCAAATAAWALGQLAG
                     GRPAYAGDLAPGWLPLAAAALAAAGIALRSDERLRTPRLGRALSWGGLLLLLWAASGL
                     PLDLLRVTSLLVPGLMPPGVDWPGLASRALALAALVVLARQVLARADDPESARDVGSS
                     PPVRWYGYAAFALALPYPVLKTWWALGGSFGLRWPGAEGLDGSFALWLPAVPWLLAAA
                     LSLLLASPPRWMPRRLLLTAGWSATVVVATIGPAAFWSLVGGLIGGDADFGAMAGWVF
                     ALFYGSWFLWAIAAGAATRAYQLRTAALRTPTAA"
     gene            complement(4927..5973)
                     /locus_tag="VFZ86_12715"
     CDS             complement(4927..5973)
                     /locus_tag="VFZ86_12715"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX5643179.1"
                     /translation="MTSVVSEAGEVVEAGPVRDATAAPRALPRSGFAWALLSGVFWGA
                     DGVVLGVALAMTPFIGAAALVAPLAMAALHDGLSTGWMLAFNAATGRLRQLPRSLRSR
                     HGLVLVAAAVIGGPIAMSGYLFGIKYAGPAYTLAISATYPAVGAVLSRIFLRERVTRR
                     GWLGIAVTVAGAVVVTYTPPDGAAPLFYLGIALAAVATLGWGVEGVLAIHAMKAIEPV
                     VAGTLRMATSFVVYLCVVLPLAGGLGVFAAAFGQTSFWIVLGAAAAGAASYLTYYAAN
                     HLVGASRAMPLNSLYAVWAIVFSVVLTGLHPSVQLFAGVLITFGGALLVVSSAPRSNG
                     DLAETEAIFPPATR"
     gene            complement(5970..6131)
                     /locus_tag="VFZ86_12720"
     CDS             complement(5970..6131)
                     /locus_tag="VFZ86_12720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643180.1"
                     /translation="MLPYHPLGAHKYDALDLAWTPFAKPEPAQLEEAAAYVAGLARGV
                     DCVVTQGLG"
BASE COUNT          935 a         2325 c         2332 g          886 t
ORIGIN      
        1 ccgcatgcat ccagggcggc gcgcgggtcg cgcgagcata accgaccggt aaccccaggc
       61 gcaatcacgc gatggcgccg ccgcgggcgt cagcctgccg cgatcagcgc cacgcccgcc
      121 aaggtcgcgg cgacccccac cagctgcgcc cgcgccaggc gttcgcgcag gaacgcgtag
      181 gccaggaggg cgaggaagac cggatacacc gaaccgagta ccgagacgat gctcaggtag
      241 gtcagcgtcg aggccaccga gaacagcgtg aaggcgccga cctcgaacac gccgatggcc
      301 aggagtggcg cggcggcccc cctcgtgtat ctcagggccg gccgcacgac caggaaggcc
      361 agcgcgaaca cggtcgcgga ggtgaggcgc gagagggcga tgctccagta cgggtccgcc
      421 tccgccccct gcgcgtagaa gaccatgacg aagccgtagg ccgtgccgga cagcaacgcc
      481 aggccgatgc tcactctgct cgtggccgtg tggtgctcgc cggcgctctt ctcccgcgag
      541 gcaaggaaga tgccgccgag tgcgatcgcg atccctgcca gctgcagtgc cgacgggcgc
      601 tccccgcgtg cgagaccgac gatcaccggc accgcgacgc tgaggctgaa gatgggcccg
      661 atgatgctca tcacgccgac ggcgagcgcc tggtagtagg cggcgagggt gacgaccatg
      721 agcaggccgg cggcaaacgc cgggagcagc acgtcctgcg ggagcggctc gccgcgaacg
      781 agcacgatca gcgccatcag gagcagcccg gtgggctggc tgacgacgat caccgtccac
      841 agcgaggtcc gccgcgtctg gctgccgccc agaaagtcgc ccgtgccata cgacaggctg
      901 gcggccagag cgaggacgac ggcgagcatg ggcacaggct accagagccc actccacgct
      961 cagaccggcg ggtccgcctc cacaccgggc acctcgactc tcaggatgtc gaggatcacc
     1021 cgctcgatgc cgtcgaggag ctcggcgtct gccatgtccg cgagccccgc ggccggccgg
     1081 agccaggagt cggtggtgcc ccagccgagg aacagcgcga cgacgcaggc gaccgcgatg
     1141 cgggggtcga ggtccttctc ggcgcgctcg gccgggggcg cggacgcggc cgctcgctcg
     1201 gcgagcgcga tgagccgctc ggtggcctcg aaccgtccgg tggtgcggtc gtagggaaga
     1261 tcgctcatcg cgctgcgcac gatgagccgg atgtgactgc gtccgtactc cagcccccgc
     1321 cgcagcatga ggctcgtgct ctggagcagg tcgggattgt cgggcgccgc ggcgaggatg
     1381 gcgttctccc tctgcgtgag cacggcgcgg aagagttcgg ccttgctgcc gatgtagcgg
     1441 tgcacgaggg cgtgcgagac gccggcgcga gcggcgactt cacgcacgct gacggcggcg
     1501 tagccgcgct cggagaacag ctcctctgcc gcgtccagga tggccgtcgt ggtcgcttcg
     1561 cgcccgcggc gctgctgccc ctcacctggc tctgtcatct cggctccccc ctcggcggcg
     1621 tgcggtggtg gccgggtcac tgtgctcttc gcaccccgcg aggactccct cgacgctcag
     1681 gaggctgcgg atcgcggtga gctcctcgtg catgacggcc agctgggtct tgagctcacc
     1741 gagctccgcc gcggggtcga cctgcttctc gggcggcggg atcaggacgc tggcgaagat
     1801 gctcgccagc gcgccgatga tcccgacgcc ggcgatcatg acgaccgcgc cgatcacccg
     1861 cccgacggcg ccgaccggat actggtcccc gtagcccacg gtggtgatcg tcacgagcgc
     1921 ccaccagagc gcgtcgccgc cggtggcgat gttcgcctcc gccaccttgc tctcactctg
     1981 caccatcacg acgctcgaga ccgacagcac ggtcatcgcc gagatcaggg tgatgaagcc
     2041 cgcgtactgg ccacggttcg ccagcacgtc cttcgccagc ccgctctggc cctggctcct
     2101 gaacaggcgc aggacgcgga ccaggcggct gaggcgcgcg agccgcagca gcgcgccgta
     2161 gcgggagata ccgagactcg ggatcgagcc gagcagatcg agccagccgc gctcgcggaa
     2221 gaagtacgtg cgccacgagg acgcgcgctt catgcgcagc ccgaagtcga ggaggaacac
     2281 caggcacatg acgaagtcgt agatctggag caactgcacg gtcgccggcg ccagccacgg
     2341 caggagcagc agcgccatgg ccagcagcga caacaccgtg aggagcagga tgaacagctc
     2401 gtaggagttg ctgaacctgg gcttcaccgc cgactccggt gtcaacgcgc gtctccttcg
     2461 gttggcgggc ccgccgggca agccgacgag cgccccgtgc agctacggac gtgcccaaga
     2521 gttcaccttc agccgcgact accctgccgg caggggccgg gcgggtccgt cgttccagcc
     2581 cgcccggccc cttgacgctc gagcggtctc aggcggcccg gcggctcgac tcgctgccga
     2641 actgctgctg ctccagcacc tcggtgatgc ggagtcccgc gttggacacg gtccggctgg
     2701 cggagccctt ccagatctcc tccagccggc ccgcgaaggt ctctgcctca cccaggctgt
     2761 cgaactcgag ctcgccgatc accgtgttct cgtcgtccac gggtcgctcg atgctgactc
     2821 gacgtacacc ggactcctcg cggcccagcg ggtcgctgtc gaagatcttc ttccactcgg
     2881 catagtcgga gacggtgtgc cagtagttca ggacgtacat gatgctccct ctcgtccagg
     2941 atactcagga tgtccctcta taccccctga cgcgcggacg tgacggttgc cgcactcacc
     3001 ctccgctttc gggggtaggc cgccccgccc cttgcggggt accgcggccc accgaagatg
     3061 cccccgccgc acgacgcgat cgaccccgcc ggggcgacga ccgcgggggc gcggccgcgt
     3121 agcctcttcc acgagaaaga ccatgcccgc ggagcgccga gggggcgccc cgcgggaccg
     3181 catgcgggag gggatcatgg acgcagtcat cgccagagag aagcaccgga ggacggcgat
     3241 cgccgtgggc gcgctgttca tcgtcggaga catcgccggc gtcctgagcg ttcttgtgac
     3301 ccgcggtctc ctcgaaggcc cggacgtcct cacccggatc gccgcggacc agggccggct
     3361 cgcactggga gcgctgctcg tgctggtcat gggcttcgcg ctcgccatgg tcccgctcct
     3421 gatgtatccg gtgttcaaga agtacaacga agtcctggcg ctcggtaccg tcgtcttcag
     3481 gagcgcgctc gagaccgccg gctacatggc caccgccggc gccatgctgc tgctcctcgg
     3541 actgagcagg gagcccgtgg aggcggcgtc caccggcgct tcgccgttcg aggccctcgg
     3601 cgcgttcctg gtgtcggcgc agggctcgac gaccgcgtac ctcaccgcca tcgccttcgc
     3661 cctcggctcc ctcatgttct acgccctctt ctaccagtcg aggctcgtcc cgcgatggct
     3721 gtcggtctgg ggtcttgccg gcgccgtcct gtacctggcc gcgccgctgc tggacatgtt
     3781 cggccatggg ttcgggatcc tgatggcccc cctggcggta gcggagatcg tcctggcggt
     3841 ctggctgatc gccaaggggc tggatgcacc ggcgccggcc cggacgcctg cgccgcagcg
     3901 gacgcccgtg ctggatcgca cgcctgcgtg acggtcgtcg acgccggtcg agtacacgcg
     3961 gaaagggctg agaacgtcat gagtacgtcg ccgcggcatg tggtggaagc gcagaggagc
     4021 cgcccgccgg gcgggagggc cgcgccgtgg atcgcgctgg cgctctgcgc cgcggcgacg
     4081 gcggcctggg cgctggggca gctcgccggc ggacggccgg cctacgccgg ggacctcgct
     4141 ccgggctggc tccccctcgc cgccgccgcg ctcgccgcgg cggggatcgc cctgcggtcc
     4201 gacgagcgtc ttcggacgcc gcgcctgggc cgcgccctga gctggggtgg tctcctgctg
     4261 ctgctgtggg cggcgagtgg gttgccgctc gacctgctcc gcgtcacgag cctccttgtc
     4321 ccgggcctca tgccgcccgg cgtcgactgg cccgggctgg cgtcgcgagc gctggctctc
     4381 gccgccctcg tcgtgctcgc gcgtcaggtc ctggcgcgcg cggacgaccc cgagtccgcc
     4441 cgcgatgtcg ggtcctcccc cccggtccgc tggtacggct acgcggcctt cgcgctcgcg
     4501 ctgccctatc cggtgctcaa gacgtggtgg gcgctgggag gcagcttcgg gctcaggtgg
     4561 ccgggtgcgg aaggcctgga cggctcgttc gccctgtggc tgcccgccgt cccgtggctg
     4621 ctggcggcag ctctgtccct gctccttgcc tctccgccgc gctggatgcc gcgccggctg
     4681 ctgctgacgg cgggctggtc cgccaccgtc gtcgtcgcca cgatcggacc ggcggcgttc
     4741 tggtcgctgg tcggcgggct gatcggcggt gatgccgact tcggagcaat ggccggctgg
     4801 gtcttcgccc tcttctacgg cagctggttc ctctgggcca tcgccgccgg cgccgccacc
     4861 cgcgcgtacc agctgcggac tgcggccctg cggacgccca cggcggcgtg accccccgcg
     4921 ccggagtcag cgggtcgccg gcgggaagat ggcctccgtc tccgccaggt ctccgttact
     4981 gcgcggagcg ctggagacca cgagcagcgc gccgccgaag gtgatcagca ccccggcgaa
     5041 gagctgcacc gaggggtgca ggccggtgag cacgaccgag aagacgatcg cccacacggc
     5101 gtagagcgag ttgagcggca tcgcgcggct ggcgccgacg aggtggttgg cggcgtagta
     5161 cgtgaggtag gaggcggccc cggccgccgc ggctcccagc acgatccaga agctggtctg
     5221 cccgaaggcc gcggcgaaga ccccgaggcc gccggccagc ggcagcacga cgcagaggta
     5281 gaccacgaac gaggtcgcca tccgcagcgt gccggcgacc accggctcga tggccttcat
     5341 ggcatggatc gcgaggacgc cctcgacgcc ccagcccagg gtcgcgaccg ccgccagggc
     5401 gatgccgagg tagaagagcg gcgcggcgcc gtcgggcggc gtgtaggtca ccacgacggc
     5461 gccggcgacg gtcacggcga tgcccagcca gccccggcgc gtgacgcgct cgcgcaggaa
     5521 gatccgcgag agcacggccc cgaccgccgg gtaggtggca ctgatcgcca aggtgtaggc
     5581 cgggccggcg tacttgatgc cgaagaggta gcccgacatc gcgatcgggc ctccgatcac
     5641 cgccgccgcg accagcacga ggccgtgccg gcttcgcagg ctgcgtggaa gctgcctcag
     5701 gcgtccggtg gcggcgttga aggccagcat ccagccggtg gacaggccgt cgtgcagcgc
     5761 ggccatcgcg agcggggcga cgagcgccgc cgcgccgatg aagggcgtca tcgcaagggc
     5821 gacgccgagg acgacgccgt cggcgcccca gaagaccccg gaaagcagcg cccacgcgaa
     5881 gccggaccgc ggcagcgcgc ggggcgcggc cgtggcatca cggacaggtc cggcctccac
     5941 gacctccccg gcctcggaga ccacggacgt catcccagcc cctgcgtgac cacgcagtcc
     6001 acgccgcgcg cgagcccggc cacgtaggcg gctgcttcct cgagttgagc cggctcgggc
     6061 ttcgcgaacg gcgtccacgc caggtccagg gcgtcgtact tgtgcgcccc gagcgggtgg
     6121 tacggcagca actcgacgtt gcgaaggccc gggtggcggg cgacgaagtc cgcgagagca
     6181 cggccccgac cgccggatac gtggcgctga tcgcaagggt gtaggccggg ccggcgtact
     6241 tgatgccgaa gaggtagccc gacatcgcga tcgggcctcc gatcaccgcc gctgcgacca
     6301 gcacgaggcc gtgccggccc cgcaggctgc gcgggagctg cctcagccgc ccggtggcgg
     6361 cgttgaaggt gagcatccag ccggtggaca ggccgtcgtg cagcgcggcc atcgcgagcg
     6421 gggcgacgag cgccgccgcg ccgatgaagg gcgtcatcgc aagggcgacg ccgaggac
//
LOCUS       DASUMX010000267        11627 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1246281, whole genome shotgun sequence.
ACCESSION   DASUMX010000267 DASUMX010000000
VERSION     DASUMX010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 11627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11627
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1246281"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1048
                     /locus_tag="VFZ86_12725"
     CDS             <1..1048
                     /locus_tag="VFZ86_12725"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HEX5643181.1"
                     /translation="RLGREARAAVDTARAQVAAALGAGDKEIVFTAGGTESDNLALFG
                     SLARYQPGHLIVSAVEHPAVMEAARALNRQGWAVDFVPVDAHGIVDLDAYEQAFRDDT
                     RLASIMFANNVVGSIQPVVELARIAHEKGALFHTDAVQAVGSVPVDVAELGADMLSLS
                     GHKLYGPKGVGALYVKRGTRLQPILHGGGHERRLRSGTENVPGIVGLGAALTLCMEQL
                     AETRPRLEALRDRLAQGVLERIPEVRYLGHPTERLPGNVSFSVRYVEGESMLLQLDAH
                     GFMVSSGSACASGSLEPSHVVLAMGLGAEEAHGSMRISLGRENTDEEADAFLEAFPPI
                     VDKLRQMSPLYVKG"
     gene            1053..1484
                     /gene="nifU"
                     /locus_tag="VFZ86_12730"
     CDS             1053..1484
                     /gene="nifU"
                     /locus_tag="VFZ86_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HEX5643182.1"
                     /translation="MYTDLVMDHFTNPRNVGEIEDADGVGQIGNPVCGDVMRISIAVD
                     KDQIADVKFKTFGCGAAVATSSMVTEMVMGKSLTEAADISNKAVAEALGGLPPNKMHC
                     SNLAADALHLAIQDYLVKSGREAEVEKILKALPKAQEHSSH"
     gene            1520..2506
                     /gene="mnmA"
                     /locus_tag="VFZ86_12735"
                     /gene_synonym="trmU"
     CDS             1520..2506
                     /gene="mnmA"
                     /locus_tag="VFZ86_12735"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF001138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEX5643183.1"
                     /translation="MVDEGRTLETATARLWPGTDRRSCCSPGALQRAARTAAQLGLPH
                     HVLDLETEFEDAVVGPFARGYLGGETPNPCVDCNPMRLAALVALAGGLGLSRVATGHY
                     ARLVWRGGEPFVARAADRTKDQSYMLWAVPPQVLARLEFPLGDLTKAETRAAATEAGL
                     EVADEPESQEVCFAVDGYQLFLEARGVEPVSGAIVDAEGAVLGEHQGHWRFTVGQRRG
                     LGVSAAEPLYVLERRAAENEVVVGGREQLEVRSLRVRDAVDRGLGDGSGLEVQLRYRS
                     APLPVAAVEWRGRDLEVGLREPFAGLAPGQAAVFYRDDVIVGGGRVAGPTPG"
     gene            2621..3244
                     /locus_tag="VFZ86_12740"
     CDS             2621..3244
                     /locus_tag="VFZ86_12740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEX5643184.1"
                     /translation="MDSSFATFAMSTPTIVLSYAAAFFCVIVGIAAAYALFKAGGTLG
                     RVDTVLDDVDKQAVPLMQKAGTTLDGVNANLGNVDQITKDVADITDKIDAMANAVEGA
                     VSTPARKAASFSAGVQSAVSSFMRRDKPAPGAEATDAAAWTAPAAPADAATDADAAAA
                     AAAPVTGAATDAAVTVDAAADAADAAAAAAPADAAAAAAAPETDAKV"
     gene            3247..3507
                     /locus_tag="VFZ86_12745"
     CDS             3247..3507
                     /locus_tag="VFZ86_12745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643185.1"
                     /translation="MGLSTRLIVSGAVAGVALSYYVRSRHARTGEGYLTIVRRLPAHA
                     LRWVDDTRGRAQRALDEGKTAARERDQEFTRQLLAAGAPPGA"
     gene            3515..3910
                     /locus_tag="VFZ86_12750"
     CDS             3515..3910
                     /locus_tag="VFZ86_12750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643186.1"
                     /translation="MAERISLEVPATLSALSTVRMVLGGLGARLDFSLEDLDDLFLAT
                     DGLLQAALEAEELDALRVRAVVDGGSLRVSAGAFRSERLRDQVALTPGDCIDLCTLLR
                     RLVDDVVVEQEQDGSFSVVIVKNSSEPAA"
     gene            3907..4845
                     /locus_tag="VFZ86_12755"
     CDS             3907..4845
                     /locus_tag="VFZ86_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013251991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigF"
                     /protein_id="HEX5643187.1"
                     /translation="MSGRDDRRANDPMDLEALPGQARRVPDDDEEPEGSEARQGGPSA
                     DPKGRGKDDSEKLDQYLLRRYHLHGDRKAREQLITMYLPLVRSLARRYASRGEHFDDL
                     VQVGAIGLIKAIDRFDLNRGVELTTYATPNIVGEIKRYFRDKGWSVRVPRGLQELNIR
                     LNKVIDELVPKLQRSPTINELAEAANATPEEVLEALETSQAYNSVSLQASPNGESGEE
                     DAGLIDYLGGEEEAYDTMEDRTTLAPGFAKLDKRERYILHLRFFEGLTQSQIAERVGI
                     SQMHVSRLIRRSLEKLREEIGDLDNPLPPRSSDDAR"
     gene            4881..7508
                     /gene="alaS"
                     /locus_tag="VFZ86_12760"
     CDS             4881..7508
                     /gene="alaS"
                     /locus_tag="VFZ86_12760"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEX5643188.1"
                     /translation="MNAADIRDLFRDFFIARGHLAGEPASLVPAGDASVLFTTAGMQQ
                     YKPYFLGIGTPPSTRITTCQRSFRTSDVENVGKTARHLTFFEMLGNFSFGDYFKAEAI
                     QWSLELSDQLGIPRDKIWVSVFGGDDQVPADEEAVQLWKAHGFGDERIVRLGRSDNFW
                     GPAGPTGPCGPCSELYYDFGPEMGCDDPDCKPGCDCDRYLEYWNLVFVQYDMDEAGGL
                     TPLPKGSIDTGMGLERIASISQGVTNVFETDLFRPLVERGAELAGVEAGASPAIVRAL
                     RTMAEHARGASFLIMDGVLPGNDGRDYVLRRIIRRAVQQAVAIGVEKPFLATLTDTVV
                     EHMSGAYPALVEAQPEIRRVVHEEEVRFRRTLDQGMGILEEALRRARDAGTELPAEVA
                     FELHDTYGFPFDLTREIAAEEEMTVDEERFGHLMEEQRERARAAQKEGAFASGPGEVD
                     DFQRRTTEVRADFVGYERLEVFTVVRALGELGGGRVAVKLAESPFYAEGGGQVADTGW
                     IHTDSGKLEVESVVKFENDQVVVARQVEGKVVEGERAKAMVNAVRRHQTACNHTATHL
                     LHNALRIVLGEHVRQGGSMVRPERLRFDFAVREAPTAEQLRQVEDLVNRRITENHPVR
                     PFVTTKEYAAEIGALAFFEEKYGEFVRVLEIDDFSRELCGGTHVSSTSQIGLFKITSS
                     QSVGANTRRIEAITSAAAVEHYRGLEHEWEALSGELKVRPDRLPATVGKLAEHVRDLE
                     KKLKSAESGERRDLAAELVAAAVEGDGVSVVAGSPQVETTDELLTLADEVRARRPDCV
                     ALLVSDVDGRVAAVVAASDAAVGRGVKAGDVMKAMLPAIGGKGGGKPTLARGGGPEVG
                     GIEAALAAGVAAVKDALGA"
     gene            7509..7904
                     /gene="ruvX"
                     /locus_tag="VFZ86_12765"
     CDS             7509..7904
                     /gene="ruvX"
                     /locus_tag="VFZ86_12765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEX5643189.1"
                     /translation="MKVVALDHGTVRTGVAVSDQTGTLARPLTVVARVGTDAGFAALL
                     ELLQSEEPARIVVGLPLSLDGGEHGQARLARAFAARLAEAVEVPVVLFDERFTSRLAD
                     QRGGTAARDARAAATLLEDYLRTVHDQGT"
     gene            7888..9075
                     /gene="mltG"
                     /locus_tag="VFZ86_12770"
     CDS             7888..9075
                     /gene="mltG"
                     /locus_tag="VFZ86_12770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEX5643190.1"
                     /translation="MTRGPDDAAGGPRPATGERPPGRGWTRYEPRAPSVADDDSGMPR
                     GRGTRREDGPRRHRGRLIGYLLVAVFALVIGAGVGYARAYFSDGEVGGAVTVVVEEGS
                     SLRAIATELERQGVVKHARAFEIRAEADGYATRFMPGTYSFRVNEPYDVLVATLLRGT
                     KPATVKVAIPEGTTLRQAAEIVSGEISAVSEREYIEAARDDPPPFALEGYKKGSTLEG
                     MLFPATYEVLPREVSAESFVKTQLEAFDANFAEVDMERARAADLTEYDVVIIASMIDR
                     EAMVGAERAVVAAVIWNRLRKDMLLQIDATIQYALGETKPLLTFDDLEIESPFNTYKY
                     AGLPPTPISNPGLAALKAAANPTDDEYLYYVARNDGTGRHYFSTTYEQFLADQAKAAA
                     NGQ"
     gene            9198..10007
                     /locus_tag="VFZ86_12775"
     CDS             9198..10007
                     /locus_tag="VFZ86_12775"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011405001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEX5643191.1"
                     /translation="MGDPVSHSLSPRLHNAAFAALGLDYVYVPLPVRAADVGAAVKGL
                     AALGLRGANVTIPHKGAVVPFLDELSEDARLVEAVNTIVVEGERLRGYNTDVEGVRGA
                     LAAAAGDALRGEPALVLGAGGAARAAALALSRLGCPLTVVNRTPAKAERLAALITAGV
                     PGARCAWLPLSALTERDVTRQRVVVNATSLGMTGEGKVPALLADNVTAGQVVFDAVYA
                     SAPTDFLVAAQARGATVIDGLTMLLGQAAEAFSLWTGVPAPLEVMRDAVQR"
     gene            9991..>11627
                     /locus_tag="VFZ86_12780"
     CDS             9991..>11627
                     /locus_tag="VFZ86_12780"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX5643192.1"
                     /translation="MRSNDEKRTGKKRNALTGRGGDELEPIIDPVESYLLDELSLEPE
                     SPADAVDAYLSDGPGRNGDGLATAEAMDPGDGHTEDEGPEDIVRSQRTSKLRLGDILK
                     EMGLVTDEQVVSALGRQKETRKRLGQLLLEDGVVTQLDLTKALAQKFGVSFLDLTETR
                     FDGAATAYIDEKLARRYGAVPVRFLDDNTLLVAMIDPQNLPAQEDLAIITGFPIQPAI
                     ASEEDVFGAIAKIYREKAEVGENTDEIGTGIDEDSLTDIRDATEEAPIVKLVNSVIAQ
                     SVDDAASDIHFEPQAKELMVRFRVDGVLHEIMSIPRRMQSGVISRLKIMADLDIAERR
                     VPQDGRIGLMVGGKPIDMRVATLPTVYGEKIVMRLLDKSNVMLDLEDLGFAEKALKRF
                     QKSFTKPYGAILVTGPTGSGKSTTLYAALNILNNPEKNIITVEDPVEYRLTGINQVQV
                     NTRAGMTFAAALRSILRCDPDIVMVGEIRDAETAENAIQAALTGHLVLSTLHTNDAPS
                     AVTRLVEMGIEPFLVGSALDAVLAQRLCRTLCGHCKEA"
BASE COUNT         1793 a         4173 c         4087 g         1574 t
ORIGIN      
        1 ccggctgggc cgggaggcgc gcgcggcggt cgatacggcg cgcgcccagg tggccgccgc
       61 cctcggcgcc ggcgacaagg agatcgtctt cactgccggc ggcaccgagt ccgacaacct
      121 cgccctcttc ggcagtctcg cccgctacca gcccggccat ctcatcgtga gcgcggtcga
      181 gcatcccgcc gtcatggagg ccgccagggc gctcaaccgc cagggatggg cggtcgactt
      241 cgtgccggtg gacgcccacg gcatcgtcga cctggacgcc tacgaacagg cgttccgcga
      301 cgacacgcgt cttgcctcca tcatgttcgc gaacaacgtg gtcggctcga tccagccggt
      361 cgtcgagctc gcgcgcatcg cgcacgagaa gggggccctc ttccacaccg acgcggtgca
      421 ggcggtcggg tcggtgcccg tcgacgtcgc cgagctgggc gccgacatgc tgagcctctc
      481 cggccacaag ctctacgggc cgaagggcgt aggcgcgctg tacgtgaagc gcggcacgcg
      541 cctgcagccc atcctgcacg gcggcggtca cgagcgccgc ctgcgctccg ggaccgagaa
      601 cgtccccggc atcgtcgggc tcggcgcggc cctcacgctg tgcatggagc agctcgccga
      661 gacacgtccc cggctcgagg cgcttcgcga caggctcgcg cagggcgtgc tggagcgcat
      721 ccccgaggtg cgctatctcg gtcaccccac ggagcggctg ccgggcaacg tctccttcag
      781 cgtgcgctac gtggagggcg agtcgatgct gctgcaactc gacgcgcacg gcttcatggt
      841 ctcgagcggc tccgcctgcg cgtccggatc gctggagccc tcacacgtcg tgctcgccat
      901 gggtctgggc gccgaggagg cgcacggaag catgcgtatc agtctcggga gggagaatac
      961 ggacgaggag gccgacgcct tcctggaagc cttcccgccg atcgtcgaca agctgcggca
     1021 gatgagcccg ctgtacgtga aaggttgagc agatgtacac cgacctggtg atggaccact
     1081 tcaccaatcc gcgcaatgtg ggggagatcg aggacgccga cggcgtcggc cagatcggca
     1141 atccggtctg cggggatgtc atgcgcatca gcatcgccgt cgacaaggac cagatcgccg
     1201 acgtcaagtt caagaccttc ggctgcggcg ccgccgtggc gacctcgagc atggtcaccg
     1261 agatggtcat gggcaagagc ctcaccgagg ccgccgacat ctcgaacaag gccgtggccg
     1321 aggccctcgg cggcctgccg ccgaacaaga tgcactgctc caacctggcg gccgacgcgc
     1381 tgcacctggc gatccaggac tacctcgtga agagcggccg tgaggccgag gtcgagaaga
     1441 tcctcaaggc actcccaaaa gcgcaagagc actcgtcgca ctgagcggcg gggtcgatag
     1501 cgccgtcgcg gcgctcaggc tggtcgacga gggccgcacc ctcgagacgg ccaccgcgcg
     1561 actctggccg gggacggacc ggcgcagttg ctgctccccc ggcgccctgc agcgcgcggc
     1621 gcgcacggcc gcgcagctcg gtcttccgca ccacgtcctc gacctcgaaa ccgagttcga
     1681 ggacgcggtc gtcggcccct tcgcgcgcgg ctacctgggc ggtgagaccc ccaatccctg
     1741 cgtcgactgc aacccgatgc gtctcgcggc gttggtcgca ctcgccggcg gactgggcct
     1801 gtcgcgcgtc gccaccggcc actacgctcg cctggtgtgg cgcggcggcg agccgttcgt
     1861 ggcccgcgcc gccgaccgga ccaaggacca gtcgtacatg ctgtgggccg tgccgccgca
     1921 ggtgctggca cgcctcgaat tcccgctcgg cgacctcacc aaggcggaga cgcgcgcggc
     1981 cgcgacggag gccggtctcg aggtggcgga cgagccggag agtcaggagg tctgcttcgc
     2041 cgtcgacggc taccagctgt tcctggaggc gcgcggcgtc gagccggtga gcggcgctat
     2101 cgtcgacgcg gagggagccg tcctgggaga gcatcaggga cactggcgct tcaccgtcgg
     2161 gcagcggcgg ggactcggcg tgagcgccgc cgagccgctg tacgtgctgg agcggcgggc
     2221 cgccgagaac gaagtggtcg tcggcggccg cgagcagctc gaggtccgct ccctgcgcgt
     2281 ccgcgacgcg gtcgaccgcg gcctgggtga cgggagcggg ctcgaagtgc agttgcgcta
     2341 ccgctcggcg ccgctcccgg tggccgccgt cgagtggcgc ggccgggacc tcgaggtcgg
     2401 gttgcgcgag ccgttcgcgg gtctcgcgcc cgggcaggcg gccgtgttct accgtgacga
     2461 tgtgatcgtc ggcgggggac gcgtcgccgg ccccacgccc ggctgacaga gcgtcccggc
     2521 gcggcttccg cacggcgtgc tcccaacgaa gtcagcggga gcggtatgat acgcttcgga
     2581 gcgtaccgaa gcggcatctg acccccgaaa ggagcgctgc atggactcct cgttcgcgac
     2641 cttcgcgatg agcactccga ccatcgtcct gagctacgcc gcggcgttct tctgcgtgat
     2701 cgtgggcatc gccgccgcct atgccctgtt caaggccggc gggacgttgg gccgtgtcga
     2761 caccgtcctg gacgacgtgg acaagcaggc cgttccgctg atgcagaagg ccgggaccac
     2821 gctcgacggc gtcaacgcca acctgggcaa cgtcgaccag atcaccaagg acgtcgccga
     2881 catcaccgac aagatcgacg cgatggccaa cgccgtcgag ggggccgtga gcaccccggc
     2941 ccgcaaggca gcctcgttca gcgccggcgt gcagagcgcc gtctccagct tcatgcgccg
     3001 cgacaagccg gccccgggcg ctgaggcaac cgacgcggcc gcgtggacgg cgccggcggc
     3061 gcctgccgac gccgccaccg acgccgacgc ggctgctgcc gccgccgcgc ccgttaccgg
     3121 cgccgcaacg gatgccgcgg tcaccgtcga tgccgcagcg gatgccgccg acgcggccgc
     3181 cgctgccgcc cccgccgacg cggccgccgc cgcggcggct ccggagacgg acgcgaaggt
     3241 ctgatcatgg ggctctccac caggctcatc gtcagcggcg ccgtggccgg cgtcgccctc
     3301 tcgtactacg tgcgctcgcg ccacgctcgc accggcgagg gctacctcac catcgtccgc
     3361 cggctgccgg ctcacgccct gcgctgggtc gacgacaccc gcgggcgggc gcagagggct
     3421 ctcgacgagg gcaagaccgc ggcacgggag cgcgatcagg agttcacccg gcagctgctc
     3481 gccgccggcg cgccgcccgg cgcctgaggt cgccatggct gagaggatct ccctcgaggt
     3541 gccggcgacg ctgagcgccc tctccaccgt gcgcatggtg ctcggcggtc tcggtgcgcg
     3601 cctcgacttc tcgctcgagg atctggacga cctcttcctg gcgacggacg gccttcttca
     3661 ggcggcgctc gaggcggagg agctcgacgc tctccgcgtc cgcgcagtcg tcgacggcgg
     3721 ctcgctgcgc gtctccgcgg gagcgttccg ctcggagcgg ctgcgcgatc aggtcgcctt
     3781 gacccccggc gactgcatcg acctgtgcac gctgctccgg cgcctcgtcg acgacgtggt
     3841 cgtggagcag gagcaggacg gctcgttcag cgtggtcatc gtcaagaaca gctcggagcc
     3901 ggcggcgtga gcgggcgaga cgacaggcgc gccaacgatc ccatggacct cgaggccctg
     3961 ccgggccagg cgcggcgcgt cccggacgac gacgaggagc cggagggctc ggaggcgcgg
     4021 caggggggac cctccgccga cccgaagggc cgcggcaagg acgactccga gaagctcgac
     4081 cagtacctgc tgcgccgcta tcacctccac ggcgaccgca aggcgcgcga acagctcatc
     4141 accatgtacc tcccgctggt gcgctcactc gcgcgccgct acgcgtctcg cggcgagcac
     4201 ttcgacgacc tggtccaggt cggcgccatc ggcctcatca aggccatcga ccgcttcgac
     4261 ctgaacaggg gcgtggagct caccacgtac gccacgccca acatcgtcgg cgagatcaag
     4321 cgctacttcc gcgacaaggg ctggtcggtg cgcgtgccgc gcggcctgca ggagctcaac
     4381 atccggctca acaaggtgat cgacgagctg gtgcccaagc tgcagcgctc gccgacgatc
     4441 aacgagctgg cggaagccgc gaacgcgacg cccgaagaag tgctcgaggc gctcgagacc
     4501 agccaggcct acaactcggt gtcgctgcag gcctccccca acggcgagtc gggcgaggag
     4561 gacgccggcc tcatcgacta cctgggcggg gaagaagagg cctacgacac catggaggac
     4621 cgcacgacgc tcgcgcccgg cttcgccaag ctcgacaagc gcgagcggta catcctccac
     4681 ctgcgcttct tcgagggact gacgcagagt cagatcgccg agcgcgtggg tatatcgcag
     4741 atgcacgtct cgcgtctcat cagacgctcg ctcgagaagt tgcgggagga gatcggcgat
     4801 ctcgacaacc cgctgcctcc gcgttcgtcc gacgacgcac gctgaggcca taccgacatc
     4861 cacgcaccga ggactccccc gtgaacgctg ccgacatccg tgacctgttc cgagacttct
     4921 tcatcgcccg cgggcacctg gcgggcgagc ccgccagcct cgtgcccgct ggcgacgcca
     4981 gcgtgctgtt cacgaccgcc gggatgcagc agtacaagcc ctacttcctg gggatcggga
     5041 cgccgccgtc cacgcgcatc accacctgcc agcgctcctt ccgcacctcg gacgtcgaga
     5101 acgtggggaa gaccgcgcgg cacctcacct tcttcgagat gctcggcaac ttctcgttcg
     5161 gcgactactt caaggccgag gcgatccagt ggtcgctgga gctctccgac cagctcggga
     5221 tcccgcgcga caagatctgg gtgagtgtct tcgggggtga cgaccaggtg ccggcggacg
     5281 aggaggcggt gcagctgtgg aaggcgcacg gcttcggcga cgagcgcatc gtccgccttg
     5341 gccgcagcga caacttctgg ggcccggccg gtcctaccgg accctgcggg ccctgctccg
     5401 agctctacta cgacttcggg cccgagatgg gctgcgacga ccccgactgc aagccggggt
     5461 gcgactgcga ccgctacctc gagtactgga acctcgtctt cgtgcagtac gacatggacg
     5521 aggccggcgg gctgacgccg ttgccgaagg gcagcatcga caccggcatg gggctggagc
     5581 gcatcgcgag catctcccag ggcgtcacca acgtgttcga gacggacctg ttccggccac
     5641 tggtcgagcg cggcgcggaa ctggccggcg tcgaggccgg agcctcgccg gcgatcgtgc
     5701 gcgccctgcg gacgatggcc gagcacgcgc gcggcgcgtc cttcctgatc atggacggcg
     5761 tcctgcccgg caacgacggc cgcgactacg tgctgcggcg catcatccgc cgcgccgtgc
     5821 agcaggcggt cgcgatcggc gtggagaagc ccttcctggc gaccctcacc gacacggtcg
     5881 tggagcacat gagcggcgcc taccccgccc tcgtcgaggc gcagccggag atccgccgcg
     5941 tcgtccacga ggaggaggtg cggttccgcc gcaccctcga ccagggcatg ggcatcctcg
     6001 aggaggccct gcgccgtgcc cgcgacgccg ggaccgagct ccccgcggag gtcgccttcg
     6061 agctccacga cacctacggg ttcccgttcg acctcacgcg cgagatcgcc gcggaggagg
     6121 agatgaccgt ggacgaggag cgtttcgggc acctgatgga ggagcagcgc gagcgcgccc
     6181 gcgccgctca gaaggagggc gcgttcgcct ccggcccggg cgaggtggac gacttccagc
     6241 ggcggacgac cgaggtgcgc gcggacttcg tcggctacga gcgcctcgag gtcttcacgg
     6301 tggtgcgcgc gctcggcgag ctcggcggcg gccgcgtggc ggtcaagctc gcggagtcgc
     6361 ccttctacgc cgagggcggc gggcaggtcg ccgacaccgg ctggatccac accgactccg
     6421 gcaagctcga ggtcgagagc gtcgtcaagt tcgagaacga ccaggtcgtg gtggctcgcc
     6481 aggtggaggg caaggtcgtg gagggcgagc gcgccaaggc catggtcaac gcggtgcggc
     6541 gccaccagac cgcctgcaac cacacggcga cgcacctgct gcacaacgcc ctgcgcatcg
     6601 tgctcggaga gcacgtccgg cagggcggct ccatggtgcg ccccgagcgc ctgcgcttcg
     6661 acttcgccgt gcgcgaggcg ccgacggcgg agcagctgcg tcaggtcgag gacctcgtca
     6721 accggcgcat caccgagaac catcccgtgc ggccgttcgt gaccaccaag gagtatgcgg
     6781 ccgagatcgg cgccctcgcg ttcttcgagg agaagtacgg cgagttcgtg cgcgtcctcg
     6841 agatcgacga cttcagccgc gagctctgcg gcggcacgca cgtctcgtcc acctcccaga
     6901 tcggcctctt caagatcacc tccagccagt cggtgggggc caacacgcgg cgcatcgagg
     6961 ccatcacctc ggccgcggcg gtcgagcact accgcggcct cgagcacgag tgggaagcgc
     7021 tctccggcga gctcaaggtg cggccggacc ggctcccggc caccgtcggg aagctcgccg
     7081 agcacgtgcg cgacctcgag aagaagctga agtcggcgga gagcggcgag cgccgcgacc
     7141 tggccgccga gctcgtcgcc gcagccgtgg agggcgacgg cgtgagcgtc gtggccggct
     7201 cgccgcaggt ggagacaacc gacgagctgc tgacgctcgc cgacgaggtg cgcgccagac
     7261 gccccgactg cgtcgccctg ctggtgtccg atgtcgacgg ccgcgtggcg gccgtcgtgg
     7321 cggccagcga cgccgccgtc gggcgcggcg tcaaggccgg cgacgtcatg aaggccatgc
     7381 tgcccgccat cggcggcaag ggcggcggca agccgacgct ggcgcgaggc ggcggccccg
     7441 aggtgggcgg catcgaggcg gccctcgcgg ccggcgtcgc cgccgtcaag gacgccctcg
     7501 gcgcttaggt gaaggtcgtc gcgctcgacc acggcacggt gcgcaccggc gtcgccgtca
     7561 gcgaccagac cgggacgctc gcgcgcccgc tcacggtcgt cgcgagggtc ggcaccgacg
     7621 ccggcttcgc ggcgctcctc gagctcctcc agagcgagga gcccgcgcgc atcgtggtag
     7681 gcttgccgct gtcgctcgac ggcggagagc acgggcaggc gcgcctcgcg agagccttcg
     7741 cggcgcgcct cgccgaagcc gtcgaggtgc ccgtcgtgct gttcgacgag cgcttcacct
     7801 cccggctcgc ggaccagcgc ggagggacgg cggcgcgcga cgcgcgcgcc gcggcgacgt
     7861 tgctcgagga ctacctcagg acggtgcatg accaggggac ctgacgacgc ggccggcggg
     7921 ccgcggcccg ccaccgggga gcgtccgccc ggccggggct ggacgcgcta cgagccacgg
     7981 gcgccgtccg tcgcggacga cgattcgggc atgccgcggg ggcgcggcac gcggcgcgag
     8041 gacggccccc gccggcatcg cggccgcctc atcggctacc tgctcgtcgc ggtcttcgcg
     8101 ctggtgatcg gcgccggcgt cggctacgcg cgcgcctact tctccgacgg cgaggtcggc
     8161 ggcgccgtga ccgtggtcgt cgaggagggc tccagcttgc gcgccatcgc caccgagctg
     8221 gagcggcagg gcgtggtcaa gcacgcccgc gccttcgaga tccgcgccga ggcggacggc
     8281 tacgccacgc ggttcatgcc gggcacctat tccttccgcg tgaacgagcc ctacgacgtc
     8341 ctcgtcgcca cgctgctcag gggcaccaag ccggcgacgg tgaaggtcgc catcccggag
     8401 gggacgacct tgaggcaggc cgccgagatc gtctccggcg agatctcggc cgtctccgaa
     8461 cgcgagtaca tcgaggcggc tcgcgacgat cctccgccgt tcgccctcga gggctacaag
     8521 aagggctcga cgctcgaggg catgctcttc ccggccacct acgaggtcct gccgcgggag
     8581 gtctccgccg aatcgttcgt gaagacgcag ctcgaggcgt tcgacgccaa cttcgccgag
     8641 gtggacatgg agcgggcgag ggcggccgac ctcaccgagt acgacgtggt gatcatcgcg
     8701 tccatgatcg accgcgaggc gatggtgggg gccgagcgcg ccgtcgtcgc ggcggtcatc
     8761 tggaaccgcc tccgcaagga catgctgctg cagatcgacg cgaccatcca gtacgcgctg
     8821 ggcgagacca agccgctgct gacgtttgac gacctggaga tcgagtcgcc gttcaacacg
     8881 tacaagtacg cgggtctgcc gccgacgccc atctccaacc ccggcctcgc ggcgctcaag
     8941 gccgccgcga acccgacgga cgacgagtac ctctactacg tcgcgcgcaa cgacggcacg
     9001 gggcggcact acttctccac cacgtacgag cagttcctcg ccgaccaggc gaaggccgct
     9061 gccaacggcc agtagcgcgt atcgaccgcg cgggcaggag cccggcggcg cgcggccaat
     9121 gtaggggtac gccggcgctc ccgtgccgga cggagggggg caccacggtc aggggcacca
     9181 cacacgtcat cggcatcatc ggcgaccccg tgtcgcactc gctctcgccg cgtctgcaca
     9241 acgcggcgtt cgcggcgctc ggcctcgact acgtctacgt gccgctgccg gtgcgcgccg
     9301 cggatgtcgg cgccgcggtc aagggcctgg cggccctcgg cctccgcggc gccaacgtca
     9361 ccatcccgca caagggggcg gtcgtcccct tcctggacga gctctccgag gacgcccgcc
     9421 tcgtggaggc cgtgaacacc atcgtcgtcg agggtgagcg gctgcgtggg tacaacaccg
     9481 acgtcgaggg ggtccgtggg gcgctcgcgg ccgccgccgg cgacgcgctg cgcggcgagc
     9541 cggcgctcgt ccttggcgcc ggaggggccg cgcgcgccgc cgcactcgcc ctctcccgcc
     9601 tcggctgccc gctcaccgtc gtcaatcgga cgcccgccaa ggcggagcgc ctggcggcgc
     9661 tcatcacggc gggcgtcccc ggcgcgcgct gcgcgtggct tccgttgtcc gccctgacgg
     9721 agcgggacgt gacccggcag agggtcgtcg tcaacgcgac ctcgctgggc atgacgggcg
     9781 agggtaaagt ccctgccctc ctcgccgata acgttacggc ggggcaagtc gtattcgatg
     9841 cggtatacgc ttccgcgccg acggacttcc tcgtggcggc gcaggctcgt ggggctaccg
     9901 tcatcgacgg cctcacgatg ttgctgggac aagcggccga ggcgttcagc ctgtggacgg
     9961 gtgtgccggc gccgctggaa gtgatgcgag atgcggtcca acgatgagaa gcgcaccggg
    10021 aagaagcgca acgccctgac cggccgcgga ggagacgaac tggaaccgat catcgatcct
    10081 gtcgagagct accttctgga cgagctgtcg ctcgagccgg agtctccggc cgacgccgtg
    10141 gacgcgtacc tgagcgacgg ccccggccgc aacggcgacg ggctggccac cgccgaggcg
    10201 atggatcccg gcgacggcca cacggaggat gagggaccgg aagacatcgt ccgcagtcag
    10261 cgcaccagca agctccgcct cggcgacatc ctcaaggaga tggggctcgt cacggacgag
    10321 caggtggtga gcgcgctggg ccgccagaag gagacgcgca agcgcctcgg ccagctgctc
    10381 ctcgaggacg gcgtcgtcac ccagctcgac ctgaccaagg cgctggcgca gaagttcggc
    10441 gtgagcttcc tcgatctcac cgagacgcgc ttcgacggcg ccgccacggc gtacatcgac
    10501 gagaagctgg cacggcgcta cggcgccgtg ccggtccgct tcctcgacga caacaccctc
    10561 ctcgtggcga tgatcgaccc gcagaacctg ccggcgcagg aggacctcgc catcatcacc
    10621 ggcttcccga tccagccggc gatcgccagc gaggaggacg tcttcggggc gatcgccaag
    10681 atctaccgcg agaaggcgga ggtcggcgag aacaccgatg agatcggcac cgggatcgac
    10741 gaggattcgc tcaccgacat ccgcgacgcc accgaagagg cgccgatcgt caagctcgtc
    10801 aactcggtca tcgcccagtc ggtcgacgac gccgccagcg acatccactt cgagccgcag
    10861 gccaaggagc tcatggtccg cttccgcgtc gacggcgtgc tccacgagat catgtcgatc
    10921 ccgcggcgca tgcagtcggg cgtgatcagc cgcctcaaga tcatggccga cctggacatc
    10981 gccgagcgcc gcgtgccgca ggacgggcgc atcggcctca tggtcggcgg caagcccatc
    11041 gacatgcgcg tcgccaccct gccgacggtc tacggcgaga agatcgtcat gcgcctgctc
    11101 gacaagtcga acgtcatgct cgacctcgag gacctcggct tcgccgagaa ggcgctcaag
    11161 cgcttccaga agtcgttcac caagccgtac ggcgcgatcc tcgtcacggg cccgaccggc
    11221 tccggcaagt cgacgaccct gtacgccgcg ctcaacatcc tcaacaaccc cgagaagaac
    11281 atcatcacgg tcgaggaccc ggtggagtac cgcctcaccg gcatcaacca ggtgcaggtg
    11341 aacacccgcg ccgggatgac cttcgcggcg gcgctgcgct cgatcctgcg ctgcgacccg
    11401 gacatcgtga tggtcggcga gatccgcgac gcggagacgg ccgagaacgc gatccaggcc
    11461 gcgctcaccg gccacctcgt cctctcgacg ctgcacacca acgacgctcc gtcggccgtc
    11521 acccggctgg tcgagatggg tatcgagccc ttcctcgtcg gctccgcgct ggacgccgtg
    11581 ctcgcccagc ggctgtgccg caccctgtgc gggcactgca aggaggc
//
LOCUS       DASUMX010000268        19351 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_674565, whole genome shotgun sequence.
ACCESSION   DASUMX010000268 DASUMX010000000
VERSION     DASUMX010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 19351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19351
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674565"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..685)
                     /locus_tag="VFZ86_12785"
     CDS             complement(<1..685)
                     /locus_tag="VFZ86_12785"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX5643193.1"
                     /translation="MISVPGRSSSIADLCARSLEGAALDERDLEVLLRAGEEAPLVFA
                     AARELRRRHSGDAVFLYGFVYFSTYCRNECAFCFYRADNDESPRYRKSAEEVVAICRD
                     LAASGVVLLDLTMGEDPAIHDEPGHTALLDLVGAVSGETGLPVMVSPGLLPESSLREL
                     RAAGADWYALYQETHTRKLYDRLRVGQPFEERAEARKAARRSGLLVEDGLLTGIGDTA
                     ADRARSIVAM"
     gene            complement(682..1344)
                     /locus_tag="VFZ86_12790"
     CDS             complement(682..1344)
                     /locus_tag="VFZ86_12790"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX5643194.1"
                     /translation="MSDRATRSRLETTGFADAVHGRSSVRRYAPDPVPREDVEAMVGI
                     AVRAANAGNAQTWRFVAVEDAGLRGAMGQAVDEVLDEMGAWPEAAGMEKQLKAYRAYA
                     TFFAEAPLVIAVLGRPYHSLADELHERRRLPREERDRLRQRPDLQSIGGAVQLLCTAA
                     HAMGYGSCWMTAPVLAAPAVEELLGVEAPAQLAALVPVGRPRTPPRPSSRRPLEDVLE
                     FR"
     gene            complement(1423..1659)
                     /locus_tag="VFZ86_12795"
     CDS             complement(1423..1659)
                     /locus_tag="VFZ86_12795"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEX5643195.1"
                     /translation="MTTPDGAKEVACRGATVREVIDEAIAVEPRMRARIFREDGRMFA
                     GVFLNGRNINALGGMDTAVADGDKLSVVPPMSGG"
     gene            complement(1695..2207)
                     /locus_tag="VFZ86_12800"
     CDS             complement(1695..2207)
                     /locus_tag="VFZ86_12800"
                     /inference="COORDINATES: protein motif:HMM:NF024585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX5643196.1"
                     /translation="MPHVMISSKKCTGCHMCELACSAYHEGAYRPSVARLYSEVNPTS
                     GATKGHTCLQTACAKCQDACPHDAIVTKEITVKVKGEFAGKEKIGDTVKGYVLVVLDD
                     KCTNCGECYDVCPTNVIHEHPDRHVAFKCDLCDGEPQCIAFCQNPHVLAVDLKVDKKD
                     RELQPAGAPA"
     gene            complement(2236..4038)
                     /locus_tag="VFZ86_12805"
     CDS             complement(2236..4038)
                     /locus_tag="VFZ86_12805"
                     /inference="COORDINATES: protein motif:HMM:NF013481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HEX5643197.1"
                     /translation="MDFYQGKVLRLDLSAGTAAVEPLNMEWAEKYIGGKGLLLRYMWA
                     EVPPKVDPWAAENPVILMTGPFAGTNVSTASRLVVGCKSPQTGILNDSYVGGSFAPEL
                     KFAGYDAVIITGESPEPVVVTIKDDVVSFVPAGPEYWGMQTSQIEEAMRRDFDPDAKT
                     LSIGPGGEKEILWACLSTDQYHKAGRGGHGALWGHKKLKAIAVRGTGTVTVGDAKAFL
                     DDMYRIHHEFVLTEDNLWANEEGTPVLAQVMSDAGVMPTRNWSSGTFDLVENINSDAF
                     LKIRTKNRACYQCAIGCRQFHETDGIAGEGPEYETIALCGGNCGIGDMAALMKFNELC
                     DQLGLDTISSGSVVGLAMDLTEKGIKDFGMRFGETEGYLKAPALIASKEGIGADLALG
                     ARALAAKYGVPELAMEVKNMELPGYDPRGAFGMSLGYATSDRGGCHMRTYPIADEIVA
                     GALPPDSLDGKAMKVVWGNPAEGMIGENFSAVKFAGIWCDFWAVTPEQIAQIFKHAWK
                     REFSEEEVFQIGERIWNLGRLFNLREGVEPDDIPQQLYAEEGAHTTGASAGRAIGTET
                     FKQALQEFYQIRGWDQSGVPSEAKLAEIGVDVRL"
     gene            complement(4256..4555)
                     /locus_tag="VFZ86_12810"
     CDS             complement(4256..4555)
                     /locus_tag="VFZ86_12810"
                     /inference="COORDINATES: protein motif:HMM:NF023376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HEX5643198.1"
                     /translation="MDEDERHINLHATPEMMAGVYANFANVSHSDYEFSITFVRLDHE
                     SEDPGELNGVVVSRVNLSTRFMKELVDAMNDNYAKWLQAENIKNLPEAPGHDEPA"
     gene            complement(4573..5577)
                     /locus_tag="VFZ86_12815"
     CDS             complement(4573..5577)
                     /locus_tag="VFZ86_12815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HEX5643199.1"
                     /translation="MTHTVTLIPGDGTGPELAAALETVIAATGVPIVWEVHDAGLDVM
                     KEYGTPLPQHVLDSIRRTKVAIKGPITTPVGTGFRSVNVALRKELDLYACLRPSKTMK
                     GVRTRFDDIDLVVVRENTEDLYAGIEHMVTDDAAESIKIITRKGTERIARFAFEYARR
                     HGRRKVTAVHKANIMKCTDGLFLRVCGEVAERYPDIEFEDRIVDNMCMQLVQKPELYD
                     VLVLPNLYGDIVSDLCAGLIGGLGVAPGANIGDDAAVFEPVHGSAPKYAGLDKVNPTA
                     LLRSGVLLLEHLGEDAAAKAMEAAIAAVIAEGKDVTYDLGGSAGTSGMAAAIARRITG
                     "
     gene            complement(5574..6086)
                     /locus_tag="VFZ86_12820"
     CDS             complement(5574..6086)
                     /locus_tag="VFZ86_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012881720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEX5643200.1"
                     /translation="MPRGRAWLFGDDISTDHIAPGRLFHLRTDMPELAKHVLEDARPE
                     FAGEMRPGDFVVGGRNFGLGSSREHAPRIIKIAGVGAVLAQSFARIFFRNAINVGLPV
                     LQCDTSGIGELDELEIDLSAGEVRDLTTGAVIPFVPLPPVMVTILNDGGLVAHVKKHG
                     GFALPGGPEA"
     gene            complement(6095..6616)
                     /locus_tag="VFZ86_12825"
     CDS             complement(6095..6616)
                     /locus_tag="VFZ86_12825"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX5643201.1"
                     /translation="MSKQYSAPPEMVIDSSKRYTATIATDNGDIVVELFADKAPRTVN
                     NFVFLAREGYYDGVTFHRVIKGFMAQGGDPTGSGSGGPGYKFADEFHAALKHDGPGIL
                     SMANAGPGTNGSQFFITYTATPHLDGRHTVFGRVVEGMDVVEAIPERDPQRAREPGLA
                     MNTVVITEEEWVT"
     gene            complement(6630..7883)
                     /locus_tag="VFZ86_12830"
     CDS             complement(6630..7883)
                     /locus_tag="VFZ86_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010936225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEX5643202.1"
                     /translation="MRATLAEKLLAAHAGLDAVVPGQIVVCDVDVAVAQDGTGPLAIQ
                     QIQELGAERVKAPECVFFIDHAAPAPRSELANAQKTIRAFCAESGATLSDVEMGVCHQ
                     RVAESYAKPGDLVIGADSHTCMAGALGAFATGMGSTDVAVGMASGKTWLRVPETFRIE
                     VEGAFADGVEAKDLMLTLIGQLGADGATYKALEFGGPGIDAMPMPGRLTLSNMSVEAG
                     AKCGLMPSDETTRAYLAAQGREEAWRPLAADPGAAYERVVTLDLARIVPTVAKPHTVD
                     NIATAAELTGTKVDQVFIGTCTNGRLEDLRSAARILRGRRRAPGTRLIVTPASQATAR
                     AAAAEGLFEVFWEAGAVITNPTCGACVGIHDGILADGDVCISTANRNFHGRMGNPEAF
                     IYLGSPATAAASAVAGEITDPRELL"
     gene            complement(7883..9136)
                     /gene="nifV"
                     /locus_tag="VFZ86_12835"
     CDS             complement(7883..9136)
                     /gene="nifV"
                     /locus_tag="VFZ86_12835"
                     /EC_number="2.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013788675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004410 - homocitrate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HEX5643203.1"
                     /translation="MTSDLSSFVIAARTRPAEPAVRIDDTTLRDGEQTAGVVFSNHEK
                     IRIAKLLDEVGVHQIEVGIPAMGGDEKKTITEIVELGLDTSILAWNRAVVDDIRHSLD
                     CGVDAVAISMSASDIHIEHKLAKSRQWVLDQIRECVAFAKDKNLYVSVNAEDASRADL
                     EFLLRFGQAAREEGADRLRFCDTLGILDPFDTFNVISFLKKEGGLDIEMHTHDDFGMA
                     TANALAGIKAGATYVNTTVNGLGERAGNAALEEVVMALKYLGKVDVGLHTSRFRELSE
                     YVAAASARTIPAWKSIVGTNVFAHESGIHADGVIKNPLNYEAFAPEDVGLERQIVVGK
                     HSGSRTIYRKFQEFGLELTQEDCNGVLELVRKTAVELKRALFDKELMYIYQDYLGRLG
                     DAVPMGNNKGDAGAASATPATGAAT"
     gene            complement(9180..10640)
                     /gene="gatB"
                     /locus_tag="VFZ86_12840"
     CDS             complement(9180..10640)
                     /gene="gatB"
                     /locus_tag="VFZ86_12840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEX5643204.1"
                     /translation="MNGRAWQPVIGLEMHVELDTQTKMFCGCAVSKGDTPNVHTCPVC
                     LAHPGALPVVNKRAVEYAARIALALNCAVRPRNIFHRKNYFYPDLPKAYQISQYDEPL
                     ATDGWFEYWVDGELLRCRINRVHMEEDAAKLIHAGGDTGRIAGSDYSMVDFNRGGTPL
                     IEIVTEPDIPSPEAAREFLQQLRNLVMELGVSACNMEEGQIRWDANISVRPEGSAELG
                     TRTELKNMNSFRYLQQALDAEIPRQIELLEAGGAIEQETLHFDPDTGTTTPLRSKEEA
                     HDYRYFPEPDLVPLEIDPGWVEGLRATQPELPATRVERFIGQYGLSRADATVLGSSGA
                     LAGFYEEVVAAGAGAKPAANWVMGEYLAHLNAAGLEAGHGHVTAERLAKLVKLVGDGT
                     VSTSAAKDVFAKMIEERAEPEAVVEKHGLGQISDTSELEVVVAEVVAANPAQVAQYRG
                     GKQQVIGFFVGQVMKASGGRANPQVVNDLLRRTLDA"
     gene            complement(10637..12169)
                     /gene="gatA"
                     /locus_tag="VFZ86_12845"
     CDS             complement(10637..12169)
                     /gene="gatA"
                     /locus_tag="VFZ86_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEX5643205.1"
                     /translation="MRLTARDAKRLLVDGEVSSAELTKAYLEQIAAVESKVRAYILVT
                     SDNAAKYSTKVDREMQAMRGPLAGLPIAVKDNMVTEGVTTTAASKILANYQPVYTATA
                     VRQVWEDHVVMLGKTNLDEFAMGSSTENSSYQVTANPWDLGTVPGGSSGGSAAAVAAG
                     EALWALGSDTGGSIRQPAALCGIVGMKPTYGAVSRYGLIAFASSLDQIGPLTHSVYDC
                     ALLLQHIVGRDPRDATSVELPEPVRLPAAERLDGLRVGVPAEYLAVGIEPRVRARFDE
                     TLELIEELGGTCVEIRLPHTEYALPAYYIIAPAEASANLARFDGVRYGYRCADPVDLL
                     DLYERTRGEGFGAEVKRRIMIGTYALSAGYYDAYYGQAQKVRTMVRRDFDAAFESVDL
                     IASPTSPTVAFKAGSRTADPLSMYVSDICTIPANLAGLPAISIPCGLADGLPVGFQLI
                     GPAFSENRMLDAAHALEGAIGFDDVPTFRDTRKERDADGWRADWPARPDEVRFSPEDA
                     QS"
     gene            complement(12189..12470)
                     /gene="gatC"
                     /locus_tag="VFZ86_12850"
     CDS             complement(12189..12470)
                     /gene="gatC"
                     /locus_tag="VFZ86_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEX5643206.1"
                     /translation="MISEDDVRYVAKLARLRIEPDEAARMTGELTRILGHIGKMSELD
                     LSDVPPTAHVLDVAGVTRDDREAPSLPRDEALRNAPSVSDDCFRVPRMG"
     gene            complement(12601..13218)
                     /locus_tag="VFZ86_12855"
     CDS             complement(12601..13218)
                     /locus_tag="VFZ86_12855"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5643207.1"
                     /translation="MNGSDRVADDPTAQLSPTAQKLVAAARRLLARGGYEALTVEAVA
                     AEAGAYRDSVRYYFGSKAAFVAAVVDSLSHDQSLSAVAETQQAAPGGDERVHGLVAAD
                     RRLVADRDAFRDFFAILPHVVLDDELRDRVAALYDWYRDLYVEGLGDGTAVEEERLRR
                     FASLMVAMTDGLAVQQLLDPEGVELEPLFALWEAMLRQALVPGED"
     gene            complement(13285..15087)
                     /locus_tag="VFZ86_12860"
     CDS             complement(13285..15087)
                     /locus_tag="VFZ86_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017718218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5643208.1"
                     /translation="MRSVLHLGRTLRLVWRISPGWTLATVALAVVQGVLPLAGLYMIQ
                     LIVNAVTEGIAAADKSAAFAEVAWLILIAGLIGLATALARSASTLVSEAQGLVITDHI
                     SDLIHSQSIAVDLEYYENSKYYDVLHRAQQEAPYRPTKIVNDLVMVGQSGITLIGVIG
                     ILFSIHWSVGLIIVAAALPSAYVRLRYSQRLYRWETARTDADRRAWYLHWLLTDGTYA
                     KEVRLFDLGETFRGWFRELRRVLRGERLRITARRSFADLASQAVATIAVFGTFAYIAW
                     RTLQGSITLGAMMMYYTAFNTGLNSLQSMLGGVAGLYEDNLFLTYYHEFMALDPKVVD
                     PAAPRPVPRPMREGVVFEDVTFDYPDTTRTALADIDLHIRPGEVTALVGANGSGKTTI
                     IKLLCRLYDPQRGRVTLDGVDLRDLAVTELRRGISVIFQDFAKYQLSARQNIWVGNVA
                     LPQDDPAIEEAAHAAGADAVITELGAGYESMLGKWFEDGEELSIGEWQRVALARAFVR
                     DAEILVFDEPTSALDPVSEWQAFEHIRTLAKGRAVVLISHRFSTVRNADRIHIVEQGR
                     IVESGTHDELMTLDGRYASMYEVQARAYQARDMQ"
     gene            15671..17062
                     /locus_tag="VFZ86_12865"
     CDS             15671..17062
                     /locus_tag="VFZ86_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5643209.1"
                     /translation="MQDAVRQQALRESRKALDLMAKSELSRDEQEAVVGDCLANFEKY
                     VNPGILEYRKSCSTDYTAVEWRDEGNVFYDIHGKEFIDCLGGFGIYVLGHRHPTVVKA
                     VMDQLQHQALHSQELLDPMRGYASELLGMVTPGDLQYTFFCNSGTEANEGALKLAKLH
                     AKRKKANHTNGIISCLGGFHGKSFGSLSVTGKAAWREPFYPLAPNVRFIPYGDADALD
                     KELKVCDAIGLDIAAFIVEPVQGEAGARVPPGDFFPKVREICDKWGVLLIADEVQTGM
                     GRTGTLWGVDHWDVAPDIMTMGKALGGAVIPVGCFTSTPEIFEPLMENPYIHTTTFGG
                     NPMATSAVIGALHATLDEEIPRQAAEKGAWLMERFEELRERHPDLLHEVRGLGLLIGL
                     EYCSEEAGYAVATGLFGEGVLTGGTLFSAKTFRIEPPATLTQHEMDEIVARLDLVMGR
                     VQAQLGAGELAAH"
     gene            complement(17168..17944)
                     /locus_tag="VFZ86_12870"
     CDS             complement(17168..17944)
                     /locus_tag="VFZ86_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007256081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5643210.1"
                     /translation="MRLDDIAGRRVLVTAAAGGIGATIAARFAAAGAEVHICDADEDA
                     LRAYLTGHPAVTGQVADVADPEQVDEVVAAATARLGGLDVLVSNAGVAGPAAPVEDMD
                     VEGWRRTLDVNLTGTFLCCRAAVPHLKAAGGGSIAVMSSNAGTMGLPYRAPYVATKWG
                     LIGLVKTLAMELGPYGIRVNAVCPGDVEGERIRRVIALEAQHRGLTPEAVSAERVEAV
                     SLRTMVTADDVASLILFAASAAGAKISGQALLVDGNAERA"
     gene            complement(18084..18890)
                     /locus_tag="VFZ86_12875"
     CDS             complement(18084..18890)
                     /locus_tag="VFZ86_12875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643211.1"
                     /translation="MGQFILPKDIPLDRYPKPEVFLEEGKRIAEAAQQQGIIMRVMGP
                     LALHYYFPDQVDLYAKLERLGERYFTDIDYAVYGKGRKGMAKLMESLGYENDLQTMAM
                     TGQTRQIYFGGKVPMIDIFFDKLDYCHEINYAGRLELDPWSVSLADILLQKLQIWEIN
                     DKDLKDIEYLFTVADFGEDDTKKVNVKFIAERLADDWGFWYTATTNLDRVKDHVDGVN
                     ALSDDQKAKIKQVADEVRARIDREPKTRKWEKRAKKGTKKIWYNTGFSDW"
     gene            complement(18953..>19351)
                     /locus_tag="VFZ86_12880"
     CDS             complement(18953..>19351)
                     /locus_tag="VFZ86_12880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643212.1"
                     /translation="TPWDTPREMSEEDLEKHLRELCAKVKNHDTAIYNFHVPPYGYSL
                     DLCPKLDENLTMAADEKIHAGSLGAKRVIEENQPLLGLHGHIHESRGAQKAGRTLLIN
                     PGSEYSEGILKGVIVMLDKKKIKDYVFTSG"
BASE COUNT         2753 a         6520 c         6902 g         3176 t
ORIGIN      
        1 gcatggcgac gatcgagcgg gcgcggtcgg cggcggtgtc gccgatgccg gtgaggagac
       61 cgtcctccac cagcaggccg ctgcgccgag cggctttgcg ggcctcggcg cgctcctcga
      121 acggctgccc cacgcgcagg cggtcgtaca gcttccgcgt gtgcgtctcc tgatagagcg
      181 cgtaccagtc ggccccggcc gcccggagct cgcgcagcga cgattcgggc agcaggccgg
      241 gcgagaccat gacgggcagg ccggtctcgc cggacacggc gccgacgagg tcgaggagcg
      301 ccgtgtgccc gggctcgtcg tggatcgccg ggtcctcccc catcgtgagg tccagcagga
      361 cgacgcccga ggcggcgagg tcgcggcaga tggcgacgac ctcttcggca gacttgcggt
      421 agcgcgggct ctcgtcgttg tcggcgcggt agaagcagaa cgcgcactcg ttgcggcagt
      481 aggtggagaa gtacacgaag ccgtagagga agacggcgtc gccggagtgc cggcggcgca
      541 gctccctggc ggcggcgaac accaacggcg cctcttcgcc ggcgcggagc agcacctcga
      601 ggtcgcgctc gtccagggcg gcgccctcga gggagcgcgc gcagaggtcg gcgatggaag
      661 agctccggcc cggcaccgag atcaccggaa ctcgaggacg tcctcgagag gccgccgcga
      721 cgagggcctg ggcggcgtcc ggggacggcc gacgggcacc agggcggcga gctgcgccgg
      781 ggcttcgact ccgaggagtt cctccacggc cggcgcggcg agcaccggcg cggtcatcca
      841 gcaggagccg taccccatcg cgtgcgccgc ggtgcagagc agctggacgg cgccgccgat
      901 gctctggagg tcggggcgct gccgcagccg gtcgcgctct tcgcgcggga ggcggcgccg
      961 ttcgtggagc tcgtccgcga gcgagtggta gggccgcccg aggacggcga tcaccagagg
     1021 ggcctcggcg aagaacgtcg cgtaggcgcg gtaggccttg agttgcttct ccatgccggc
     1081 ggcctcaggc caggcgccca tctcgtccag gacctcgtcg acggcctgcc ccatggcgcc
     1141 gcgcaggccg gcgtcctcca ccgcgacgaa ccgccacgtc tgcgcgttgc cggcgttggc
     1201 cgccctgacg gcgatgccga ccatggcctc gacgtcctca cgcggcaccg ggtcgggcgc
     1261 gtagcggcgg acgctggaac gcccgtggac ggcgtcggcg aagcccgtcg tctcgagtct
     1321 cgatctggtg gcgcggtcgc tcatgcgtcc agtgtaccaa cgaggacggg gcgggcgctc
     1381 ctcgaggagc gcacgccccg tccatcctcc gccgccgcgg actcagccgc ccgacatcgg
     1441 cgggaccaca ctgagcttgt cgccgtcggc cacggccgtg tccatcccgc ccagggcgtt
     1501 gatgttgcgg ccgttgagga acaccccggc gaacatgcgc ccgtcctcgc ggaagatgcg
     1561 ggcgcgcatc ctcggctcca ccgcgatggc ctcgtcgatc acctcgcgca cggtggcgcc
     1621 gcggcaggcg acctccttgg cgccgtcggg cgtggtcatg ccgacgggga gtctgacgta
     1681 ggacgtcggc atcctcaggc gggcgccccg gccggctgca gctcgcggtc cttcttgtcg
     1741 accttgaggt cgaccgccag tacgtggggg ttctggcaga aggcgatgca ctgcggctcg
     1801 ccgtcgcaga ggtcgcactt gaaggcgacg tggcggtccg gatgctcgtg gatcacgttc
     1861 gtggggcaga cgtcgtagca ctcgccgcag ttggtgcact tgtcgtccag gacgacgagc
     1921 acgtagccct tcacggtgtc gccgatcttc tccttgccgg cgaactcgcc cttgaccttg
     1981 accgtgatct ctttggtcac gatcgcgtcg tgggggcagg cgtcctggca cttggcgcag
     2041 gcggtctgca ggcaggtgtg tcccttggtg gcgccgctgg tggggttgac ctcgctgtag
     2101 aggcgggcga cggaggggcg gtaggcgccc tcgtggtagg cagagcaggc cagctcacac
     2161 atgtggcagc cggtgcactt cttgctcgag atcatcacgt gaggcattgg ttctccttcg
     2221 cttcggcgtc gcgccttaga ggcgcacgtc cacgccgatc tcggcgagtt tggcttcgct
     2281 gggcacgccg ctctggtccc acccgcggat ctggtagaac tcctgcagcg cctgcttgaa
     2341 cgtctcggtg ccgatggcgc ggccggcgga cgcgccggtg gtgtgggcgc cctcctccgc
     2401 gtacagctgc tgcgggatgt cgtcgggctc gacgccctcg cgcaggttga acagacggcc
     2461 gaggttccag atgcgctcgc cgatctggaa gacctcttcc tcactgaact cccgcttcca
     2521 cgcgtgcttg aagatctgcg cgatctgctc gggcgtgacg gcccagaagt cgcaccagat
     2581 gccggcgaac ttcacggcgc tgaagttctc gccgatcatg ccctcggccg ggttgcccca
     2641 gacgaccttc atcgccttgc cgtcgagcga gtccggcggc agcgcgccgg cgacgatctc
     2701 gtcggcgatc gggtaggtgc gcatgtggca gccgccgcgg tcggacgtgg cgtagccgag
     2761 gctcatgccg aacgcgccgc gcgggtcgta gcccggcagc tccatgttct tgacctccat
     2821 ggcgagctcg ggcacgccgt acttggcggc cagggcgcgg gcgccgagcg cgaggtccgc
     2881 gccgatgcct tccttgctgg cgatgagcgc cggcgccttg aggtagccct cggtctcgcc
     2941 gaagcgcatg ccgaagtcct tgatgccctt ctcggtgagg tccatggcga ggccgacgac
     3001 gctgccgctg ctgatggtgt cgaggccgag ctgatcgcac agctcgttga acttcatgag
     3061 cgcagccatg tcgccgatgc cgcagttgcc gccgcagagc gcgatggtct cgtactcggg
     3121 accctcgccg gcgatgccgt cggtctcgtg gaactggcgg cagccgatgg cgcactggta
     3181 gcaggcgcgg ttcttggtgc ggatcttgag gaacgcgtcc gagttgatgt tctcgaccag
     3241 gtcgaaggtg cccgacgacc agttgcgcgt gggcatgacg ccggcgtcgc tcatgacctg
     3301 cgcgagcacc ggtgtgccct cctcgttggc ccagagattg tcctcggtga gcacgaactc
     3361 gtggtggatg cggtacatgt cgtcgaggaa cgccttggcg tcaccgacgg tgaccgtacc
     3421 cgtgccgcgc acggcgatgg ccttgagctt cttgtgaccc cagagggcgc cgtgaccgcc
     3481 acgtccggcc ttgtggtact ggtcggtgga gaggcaggcc cagaggatct ccttctcgcc
     3541 gccggggccg atggacagcg tcttggcgtc ggggtcgaag tcccggcgca tggcctcttc
     3601 gatctgggac gtctgcatgc cccaatactc gggcccggcg ggcacgaagg agacgacgtc
     3661 gtccttgatg gtgacgacga ccggctcagg tgactcgccg gtgatgatga ctgcgtcgta
     3721 gccggcgaac ttgagctcgg gggcgaacga gccgccgacg tagctgtcgt tgaggatgcc
     3781 ggtctgcggg ctcttgcagc cgacgacgag acgcgaggcg gtgctgacgt tggtcccggc
     3841 gaacggcccg gtcatgagga tgacggggtt ctccgccgcc cacgggtcga ccttgggcgg
     3901 gacctcggcc cacatgtagc gcagaagcag acctttgccg ccgatgtact tctccgccca
     3961 ctccatgttg agcggctcga ccgcggcggt gcctgcgctc aggtccagcc tgagcacctt
     4021 gccctggtag aagtccaatg cgtcctcctg aagacgtgcg atgggatcgc catcgttccc
     4081 ccccggcagc ccgctcgcgg ctccggcggc ggaccaagga gaacgtggag tacaggctct
     4141 gtcattctag ccgacgcggt accggcgcgg caacgccgcg aaaggcgctg aatgcagggc
     4201 aaaacggagc cgaaacggga ggccgcttgg ccattctggc acgcggcggc ccggtctacg
     4261 ccggctcgtc gtgcccgggg gcctccggca ggttcttgat gttctccgcc tgcagccact
     4321 tggcgtagtt gtcgttcatc gcgtccacga gctccttcat gaagcgcgtg gagaggttga
     4381 cgcggctcac gacgacgccg ttgagctcac cgggatcctc gctctcgtgg tcgaggcgca
     4441 cgaaggtgat ggagaactcg tagtcggagt ggctgacgtt ggcgaagttg gcgtacacgc
     4501 ccgccatcat ctccggcgtg gcgtgcaggt tgatgtggcg ttcgtcctcg tccattgagg
     4561 agcctccttg cctcagccgg tgatgcggcg ggcgatggcg gcggccatgc cggacgtgcc
     4621 ggccgagccg ccgaggtcgt acgtgacgtc cttgccctcg gcgatgacgg cggcgatggc
     4681 agcctccatc gccttcgccg ccgcgtcctc gccgaggtgc tcgagcagga gcacgccgga
     4741 gcggagcagc gccgtgggat tcaccttgtc caggccggcg tacttgggcg cgctgccgtg
     4801 gaccggctcg aagacggccg cgtcgtcgcc gatgttcgcc ccgggcgcga cgccgagccc
     4861 gccgatgagc ccggcgcaca ggtcactgac gatgtcgccg tacaggttgg gcagcacgag
     4921 cacgtcgtac agctccggtt tctgcaccag ctgcatgcac atgttgtcga cgatgcggtc
     4981 ctcgaactcg atgtcggggt acctctcggc gacctccccg cagacgcgca ggaacagccc
     5041 gtcggtgcat ttcatgatgt tggccttgtg caccgcggtg accttgcggc ggccgtggcg
     5101 gcgcgcgtac tcgaaggcga agcgcgcgat acgctcggtc cccttgcgcg tgatgatctt
     5161 gatgctctcg gcggcgtcgt ccgtgaccat gtgctcgatg ccggcgtaga ggtcctcggt
     5221 gttctcgcgc acgacgacga ggtcgatgtc gtcgaagcgg gtgcgcacgc ccttcatcgt
     5281 cttggacggg cgcaggcagg cgtagaggtc gagttccttg cgcagcgcca cgttgacgct
     5341 gcggaagccc gtgcccaccg gggtcgtgat cgggcccttg atcgccacct tggtgcggcg
     5401 gatcgagtcg agcacgtgct gcgggagcgg cgtgccgtac tccttcatca cgtcgagacc
     5461 ggcgtcgtgc acctcccaga cgatggggac gccggtcgcg gcgatcacgg tctcgagcgc
     5521 ggcggccagc tcggggccgg tgccgtcccc ggggatcagg gtcacggtgt gggtcatgcc
     5581 tcgggtcctc cgggcagcgc gaagccgccg tgcttcttga cgtgggcgac gagacccccg
     5641 tcgttcagga tggtcaccat caccggcggg agcgggacga aggggatgac ggcgccggtg
     5701 gtgaggtctc tcacctcacc ggcgctcagg tcgatctcga gctcgtccag ctcgccgatg
     5761 ccgctggtgt cgcactgcag caccggcagg ccgacgttga tggcgttgcg gaagaagatg
     5821 cgcgcgaagc tctgcgccag cacggctccg accccggcga tcttgatgat gcgcggcgcg
     5881 tgctcgcggc tcgagccgag gccgaagttg cgccccccca cgacgaagtc gccgggccgc
     5941 atctcgccgg cgaactccgg ccgcgcgtcc tcgagcacgt gcttggcgag ctcgggcatg
     6001 tcggtgcgca ggtggaacag gcgcccgggc gcgatgtggt cggtgctgat gtcgtcgccg
     6061 aacagccagg cccggccgcg cggcagctcg ttgctcacgt gacccattcc tcctccgtga
     6121 tgaccaccgt gttcatggcg agcccgggct cgcgcgcccg ctgggggtcg cgctccggga
     6181 tcgcctcgac gacgtccatg ccctcgacca cacggccgaa caccgtgtgc ctgccgtcga
     6241 ggtgcggcgt ggccgtgtag gtgatgaaga actgtgaccc gttggtgccg gggccggcgt
     6301 tggccatgga gaggatgccg ggaccgtcgt gcttgagcgc cgcgtggaac tcgtccgcga
     6361 acttgtagcc cggaccgccg cttccactgc cggtcgggtc gccgccctga gccatgaacc
     6421 ccttgatgac gcggtggaac gtgaccccgt cgtagtagcc ctcacgggcg aggaacacga
     6481 agttgttgac ggtgcgtggc gccttgtcgg cgaacagctc gacgacgatg tcaccgttgt
     6541 cggtggcgat cgtcgctgtg tagcgcttgc tggagtcgat gaccatctcc ggcggcgcgc
     6601 tgtactgctt gctcacagcg tcctcctttc tagagaagct cgcggggatc ggtgatctcg
     6661 ccggccacgg ccgaggcggc cgcggtggcc ggcgagccca gatagatgaa ggcctcgggg
     6721 ttgcccatgc ggccgtggaa gttgcggttg gccgtgctga tgcagacgtc gccgtcggcg
     6781 aggatgccgt cgtggatgcc gacgcaggcg ccgcacgtgg ggttggtgat gacggcgccg
     6841 gcttcccaga acacctcgaa caggccttcg gccgcggcgg cgcgcgccgt ggcctgcgag
     6901 gccggcgtca cgatgagccg cgtgcccggg gcgcggcggc gcccgcgcag gatgcgcgcc
     6961 gccgagcgca ggtcctcgag gcggccgttg gtgcaggtgc cgatgaacac ctggtccacc
     7021 ttcgtgccgg tcagctctgc cgccgtcgcg atgttgtcca ccgtgtgcgg cttggccacg
     7081 gtcggcacga tgcgcgccag gtcgagtgtc acgacgcgct cgtaggcggc gccgggatcg
     7141 gccgcgagcg gccgccaggc ctcctcgcgg ccctgcgccg ccaggtatgc gcgcgtcgtc
     7201 tcgtcgctcg gcatgaggcc gcacttggcg ccggcctcca cggacatgtt gctcagcgtc
     7261 aggcgtccgg gcatgggcat cgcgtcgatg cccggcccgc cgaactcgag cgccttgtac
     7321 gtggcgccgt cggcgccgag ctgcccgatc agcgtgagca tcaggtcctt ggcctcgacg
     7381 ccgtcggcga aggcgccctc gacctcgatg cggaaggtct cgggcacgcg cagccacgtc
     7441 ttgccgctgg ccatgcccac ggcgacgtcg gtgctgccca tgccggtggc gaaggcgccg
     7501 agcgctcccg ccatgcaggt gtgcgagtcg gcgccgatga cgagatcgcc gggcttggcg
     7561 tagctctcgg cgacgcgctg gtggcacacg cccatctcga cgtcgctcag ggtggcgccc
     7621 gactccgcgc agaaggccct gatcgtcttc tgcgcgttgg ccagctcgct gcgcggcgcc
     7681 ggcgccgcgt ggtcgatgaa gaacacgcac tccggcgcct tgacgcgctc cgcgcccagc
     7741 tcctggatct gctggatggc gagcgggccg gtgccgtcct gggcgacggc gacgtcgacg
     7801 tcgcagacga cgatctggcc gggcacgacg gcgtcgagcc cggcgtgcgc ggccagcagc
     7861 ttctccgcca gcgtcgccct catcacgtcg ccgctccggt ggcgggggtg gcgctggcgg
     7921 ccccggcgtc acccttgttg ttccccatcg gcacggcgtc gcccagccgg ccgaggtagt
     7981 cctggtagat gtacatgagc tccttgtcga acagcgcccg cttgagctcc accgcggtct
     8041 tgcgcacgag ctcgagcacg ccgttgcagt cctcctgcgt gagctcgagg ccgaactcct
     8101 ggaacttgcg gtagatggtg cgcgagccgg agtgcttgcc gaccacgatc tggcgctcca
     8161 gcccgacgtc ctcgggtgcg aacgcctcgt agttgagcgg gttcttgatg acgccgtcgg
     8221 cgtggatgcc gctctcgtgg gcgaacacgt tggtccccac gatgctcttc cacgcgggga
     8281 tcgtccgcgc cgaggcggcg gcgacgtact cactgagctc gcggaagcga ctggtgtgca
     8341 ggcccacgtc caccttgccg aggtacttga gcgccatcac gacctcctcg agcgccgcgt
     8401 tgccggcgcg ctcgccgaga ccgttgacgg tggtgttgac gtaggtggcg ccggccttga
     8461 tgcccgccag ggcgttggcc gtcgccatgc cgaagtcgtc gtgcgtgtgc atctcgatgt
     8521 cgagcccgcc ctccttcttg aggaacgaga tcacgttgaa ggtgtcgaac ggatcgagga
     8581 tgccgagcgt gtcgcagaaa cggaggcggt cggcgccctc ctccctggcc gcctggccga
     8641 agcgcagcag gaactcgagg tcggcgcgcg aggcgtcctc ggcgttcacg gagacgtaga
     8701 ggttcttgtc cttggcgaac gccacgcact cgcggatctg gtcgagcacc cactggcggc
     8761 tcttggccag cttgtgctcg atgtggatgt cgctggccga catgctgatg gccaccgcgt
     8821 cgacgccgca gtccagcgag tgacggatgt cgtccaccac ggcgcggttc caggcgagga
     8881 tcgacgtgtc gaggccgagc tcgacgatct ccgtgatggt cttcttctcg tcgccgccca
     8941 tggccgggat gccgacctcg atctggtgca cgccgacctc gtcgagcagc ttggcgatgc
     9001 ggatcttctc gtggttgctg aacacgacgc cggcggtctg ctcgccgtcg cgcagggtgg
     9061 tgtcgtcgat gcgcacggcc ggctcggcag ggcgggtccg cgcggcgatg acgaagctgg
     9121 agagatcgct ggtcatgttc acctacctca agggacggat gtggtcgggc gtgggacggt
     9181 cacgcgtcga gggtgcgccg gagcaggtcg ttcacgacct gcgggttggc gcgcccgccg
     9241 ctggccttca tcacctggcc gacgaagaag ccgatgacct gctgcttgcc gccgcggtac
     9301 tgcgcgacct gggccgggtt ggcggcgacg acctcggcga cgacgacctc gagctcggag
     9361 gtgtcgctga tctggccgag gccgtgcttc tcgacgacgg cctcgggctc cgcgcgctcc
     9421 tcgatcatct tcgcgaagac gtccttggcg gcgctcgtcg agacggtccc gtccccgacg
     9481 agcttgacga gcttggccag gcgctcggcc gtcacgtggc cgtggccggc ctcgaggccg
     9541 gcggcgttga ggtgggcgag gtactcgccc atcacccagt tggccgccgg cttggcgccg
     9601 gccccggccg ccacgacctc ttcgtagaag ccggcgagcg cgccggagga gcccagcacg
     9661 gtggcgtcgg cacgcgagag cccgtactgc ccgatgaagc gctccacgcg cgtcgccggc
     9721 agctccggct gcgtcgcgcg cagcccctcc acccagccgg ggtcgatctc gagcggcacc
     9781 aggtcgggct ccgggaagta gcggtagtcg tgcgcctcct ctttgctgcg cagcggtgtc
     9841 gtggtcccgg tgtccgggtc gaagtgcagc gtctcctgct cgatcgcgcc gccggcctcg
     9901 agcagctcga tctgccgcgg gatctccgcg tcgagcgcct gctgcaggta gcggaagctg
     9961 ttcatgttct tgagctcggt gcgcgtgccc agctccgcgc tcccctccgg ccggacgctg
    10021 atgttcgcgt cccagcggat ctgcccctcc tccatgttgc aggcgctgac gccgagctcc
    10081 atgacgaggt tgcgcagctg ctggaggaac tcgcgcgccg cctccggcga cgggatgtcc
    10141 ggctcggtga cgatctcgat gagcggggtt ccgccgcggt tgaagtcgac catggagtag
    10201 tcggagccgg cgatgcgccc cgtgtcgccg ccggcgtgga tgagcttggc ggcgtcctcc
    10261 tccatgtgca cgcggttgat gcggcagcgg aggagctcgc cgtccaccca gtactcgaac
    10321 cagccgtcgg tggccagcgg ctcgtcgtac tggctgatct ggtaggcctt gggcaggtcg
    10381 ggatagaagt agttcttgcg gtggaagatg ttgcgcggcc ggacggcgca gttgagcgcc
    10441 agcgcgatgc gtgcggcgta ctcgacggcg cgcttgttca ccacgggcag cgcgccgggg
    10501 tgcgccaggc acacggggca ggtgtgcacg ttcggcgtgt cgcccttgct cacggcgcag
    10561 ccgcagaaca tcttggtctg cgtgtcgagc tccacgtgca tctcgagccc gatgaccggc
    10621 tgccaggcgc gaccgttcat gactgcgcgt cctcgggcga gaagcggacc tcgtccggcc
    10681 tggccggcca gtcggcgcgc cagccgtcgg cgtcgcgctc cttgcgcgtg tcgcggaagg
    10741 tgggcacgtc gtcgaacccg atggctccct cgagcgcgtg cgccgcgtcg agcatgcggt
    10801 tctcgctgaa cgcggggccg atgagctgaa agcccaccgg cagcccgtcg gcgagaccgc
    10861 acgggatgct gatcgccggg agcccggcga gattcgccgg gatggtgcag atgtcgctca
    10921 cgtacatgga cagcgggtcg gccgtgcgcg atccggcctt gaaggcgacg gtgggcgacg
    10981 tcggggacgc gatgaggtcg acgctctcga acgcggcgtc gaagtcgcgg cgcaccatgg
    11041 tgcggacctt ctgggcctga ccgtagtagg cgtcgtaata cccggcggag agcgcgtaag
    11101 tgccgatcat gatacggcgc ttgacctcgg cgccgaaccc ctcgccgcga gtgcgctcgt
    11161 agaggtcgag caggtcgacg ggatcggcgc agcggtagcc gtagcgcacg ccgtcgaagc
    11221 gggcgaggtt cgccgacgcc tcggccggcg cgatgatgta gtacgccggc agggcgtact
    11281 cggtgtgcgg caggcggatc tcgacgcagg tgccgcccag ctcctcgatg agctcgagcg
    11341 tctcgtcgaa gcgggcgcgc acgcgcggct cgataccgac ggcgaggtac tcggccggca
    11401 cgccgacacg cagcccgtcg agccgctccg ccgccggcag ccggaccggc tcgggcagct
    11461 cgacgctggt cgcgtcccgc gggtcgcggc cgacgatgtg ctgcagcagg agcgcgcagt
    11521 cgtagacgga gtgcgtgagc gggccgatct ggtcgagcga gctggcgaag gcgatgaggc
    11581 cgtagcggct caccgcgccg taggtgggct tcatgccgac gatgccgcag agggcggccg
    11641 gctggcggat ggagccgccg gtgtcgctgc cgagcgccca gagcgcctcg ccggcggcca
    11701 cggcggcggc ggagccgccg ctgctgccgc cgggcacggt gccgaggtcc catgggttgg
    11761 cggtgacctg ataggacgag ttctcggtgc tggagcccat ggcgaactcg tccaggttgg
    11821 tcttgccgag catgaccacg tggtcctccc acacctggcg caccgcggtc gccgtgtaca
    11881 ccggctggta gttggcgagg atcttcgacg cggccgtggt ggtgacgccc tcggtgacca
    11941 tgttgtcctt gacggcgatg ggcaggcccg ccagcgggcc ccgcatcgcc tgcatctcgc
    12001 ggtccacctt ggtcgagtac ttggcggcgt tgtcgctggt gacgaggatg taggcgcgca
    12061 ccttgctctc gacggcggcg atctgctcga gatacgcctt ggtgagctcg gcggatgaga
    12121 cctccccgtc cacgagcagg cgcttggcgt cgcgggcggt gagacgcagg ggatcgaggt
    12181 ccgtcacgtc agcccatcct ggggacacgg aagcagtcgt cactcactga aggcgcgttg
    12241 cgcagcgctt cgtcgcgcgg cagactcggg gcctcgcgat cgtcgcgggt cacgccggcg
    12301 acgtcgagga cgtgcgccgt cggcggcaca tcgctgaggt cgagctcgct catcttgccg
    12361 atgtgaccga ggatcctcgt cagctcaccg gtcatgcgcg ccgcctcgtc cggttcgatg
    12421 cggagacggg cgagtttcgc gacgtagcgg acgtcgtctt cggagatcat gtcgcgttcc
    12481 tcctgctggg ccgccgaagc gggacggcca tcttactgca gcctcagatg gcctacaaga
    12541 aacggaggcg gcgagcctgc gccacagccc gggggacgcc cacgccgcgg tcgcgccgcg
    12601 tcagtcctcc cccggcacca gggcctggcg gagcatggct tcccagagcg cgaacagcgg
    12661 ctcgagctcg acgccttcgg ggtcgagcag ttgctgcacg gccaggccgt cggtcatcgc
    12721 gaccatgagg gaggcgaacc ggcgcagccg ctcctcctcc accgccgtgc cgtcgcccag
    12781 accctccacg tagaggtcgc ggtaccagtc gtagagcgcc gcgacccggt cccgcagctc
    12841 gtcgtcgagg accacgtggg gaaggatggc gaagaagtcg cggaaggcgt cgcggtccgc
    12901 caccaggcgg cggtcggcgg cgaccagccc gtgcacgcgc tcgtcgccgc ccggcgccgc
    12961 ctgctgcgtc tcggcgaccg cgctcaggct ctggtcgtgg ctgagcgagt cgaccacggc
    13021 ggcgacgaac gccgccttgc tgccgaagta gtagcgcacg gagtcgcggt aggcgccggc
    13081 ctcggccgcc accgcctcca cggtgagggc ctcgtagccg ccgcgggcca gcaggcggcg
    13141 cgcggcggcc accagcttct gcgccgtcgg cgagagctgg gcggtcgggt catcggccac
    13201 tcggtcgctg ccgttcatgc tgcctcccgc gcgcatcacg ccgttcggga tcgcgacggg
    13261 tgcgttcggt cgtttgtacg aactctactg catgtcgcgc gcctggtagg cgcgcgcctg
    13321 cacctcgtac atgctcgcgt agcgcccgtc gagcgtcatc aactcatcgt gcgtgccgct
    13381 ctccacgatc cttccttgct cgacgatgtg gatgcggtct gcgttgcgca cggtggagaa
    13441 ccggtggctg atgagcacca cggcacgccc cttcgcgagc gtgcggatgt gctcgaaggc
    13501 ctgccactcg ctgacggggt cgagggcgct ggtcggttcg tcgaagacga ggatctcggc
    13561 gtcgcggacg aacgcccgcg ccagagcgac cctctgccac tcgccgatgc tcagctcttc
    13621 gccgtcctcg aaccacttgc cgagcatcga ctcgtagccg gccccgagct cagtgatgac
    13681 ggcatcggca cctgcggcgt gcgccgcctc ctcgatcgcg gggtcgtcct gcggcagcgc
    13741 cacgttcccc acccagatgt tctggcgggc cgagagctgg tacttggcga agtcctggaa
    13801 gatcacgctg ataccgcgcc gcagctcggt gaccgccaga tcccgcagat cgacgccgtc
    13861 gagcgtcacg cggccgcgct ggggatcgta gagccgacag agaagcttga tgatggtcgt
    13921 cttgccagac ccgttcgcgc ccacgagggc ggtgacctca cccggtctga tatgcaggtc
    13981 gatatcggcg agggcggtcc gcgtcgtgtc cgggtagtcg aaggtcacat cctcgaagac
    14041 gacgccctca cgcatgggcc ggggcacggg acgcggcgcc gcggggtcga cgaccttggg
    14101 atccagcgcc atgaactcgt ggtagtaggt cagaaagaga ttgtcctcgt acagccccgc
    14161 gacgccaccg agcatgctct gcagcgagtt caggccggtg ttgaaggccg tgtagtacat
    14221 catcatggcg ccgagggtga tgctgccctg cagcgtgcgc caggcgatgt aggcgaaggt
    14281 accgaacacg gcgatcgtcg ccacggcctg actggcgagg tcggcgaaag agcgccgcgc
    14341 ggtgatgcga aggcgctcgc cgcggagcac gcggcgcaac tcccggaacc agccgcggaa
    14401 ggtctcaccc aggtcgaaca ggcggacctc cttcgcgtag gtgccgtccg tgagcaacca
    14461 gtgcagatac cacgcacggc gatccgcgtc ggtacgcgcc gtctcccagc gatacaggcg
    14521 ctgcgagtag cgcagccgca cataggcgct gggcagggcg gcggcgacga tgatcaggcc
    14581 gacgctccag tggatcgaga acaggatgcc gatcacaccg atcagggtga taccgctctg
    14641 gcccaccatg accagatcgt tcacgatctt ggtgggcctg tagggagcct cctgctgcgc
    14701 ccggtgcagg acgtcgtagt acttggagtt ctcgtagtac tcgaggtcga cggcgataga
    14761 ctgcgagtgg atgaggtccg agatgtggtc ggtgatgacc agaccttgcg cctcgctgac
    14821 cagcgtggaa gcggagcgcg ccagcgccgt cgcgaggccg atcagcccgg cgatgaggat
    14881 cagccaggcg acctcggcga acgcggcgct cttgtcggca gcggcaatgc cctccgtcac
    14941 ggcattcacg atgagctgga tcatgtagag gccggcgagg ggcaggacgc cctggacgac
    15001 ggcgagcgcg accgtcgcca gcgtccagcc cggcgagatg cgccacacca ggcgcagggt
    15061 gcgtccgagg tgcaggacac tgcgcacgcc gatcctgggt gcctcttcag ccagtcagtc
    15121 cgcctttcgc gggttctccg ccgcggcggc gtccgacgga gcatcccacg cggcgacgcc
    15181 gaggctgcgc ccgggcgatc caccgcgtgc gccccgacga cggtgggcgg ccgcgagcag
    15241 gagcaggatg gcggagtcag gcgccggcaa cggccggcgc caggagacac gccgaggtgg
    15301 gcagagtcgt cgagtcacgg ccacgccaga cgataccacg tgctgcggtg cagacgagca
    15361 gcgtcacggg tggccgtcaa ggacccgaac ggctgcgagg gcaccacgcg gcaacagcca
    15421 tgtccaatga atcgggagcc caccacccgg tggccgaccg tcgcagcgcc cacctggcgg
    15481 cgtgatctca gggagtccgc gccgacatga ccgccgcatc gcgcccagct cacgacttcg
    15541 aggggatgga cgccgctgtt tgcaggaagg ccgtcatcgc gatagattga cagcatcgtt
    15601 cggttgtacg accgaactct actgcaaatc aggggattcc gcagtgatca actgtcgaca
    15661 gggggaacga atgcaggatg cagtgaggca acaggcgttg cgagaatcgc gcaaggcgct
    15721 cgacctgatg gcgaaatccg aactgtcgcg cgacgagcag gaggcggtgg tcggcgactg
    15781 tctggcgaac ttcgagaagt acgtcaaccc cggcatcctc gagtaccgca agtcctgctc
    15841 cacggactac accgccgtcg agtggcgcga cgagggcaac gtcttctacg acatccacgg
    15901 caaggagttc atcgactgcc tcggcggctt cggcatctac gtgctcggcc accggcaccc
    15961 caccgtcgtc aaggcggtga tggaccagct ccagcatcag gccctgcact cgcaggagct
    16021 cctcgacccc atgcgcggct acgccagcga gctcctcggc atggtcactc cgggcgatct
    16081 gcagtacacc ttcttctgca actccgggac ggaggccaat gagggcgcgt tgaagttggc
    16141 gaagctgcac gccaagcgca agaaggccaa ccacaccaac ggcatcatca gctgcctggg
    16201 tggcttccac ggcaagtcgt tcggcagcct gagcgtgacc ggcaaggctg cctggcggga
    16261 gcccttctac ccgctggcgc ccaacgtgcg cttcatcccc tacggggacg ccgacgcgct
    16321 cgacaaggag ctcaaggtct gcgacgccat cgggctcgac atcgccgcct tcatcgtcga
    16381 gcccgtccag ggcgaggccg gcgcccgcgt ccctcccggc gacttcttcc ccaaggtccg
    16441 cgagatctgc gacaagtggg gcgtgctgct catcgcggac gaggtgcaga ccggcatggg
    16501 ccgcacgggc acactgtggg gcgtcgacca ctgggacgtc gctcccgaca tcatgaccat
    16561 gggcaaggcg ctgggcggcg ccgtcatccc tgtcggctgc ttcacctcca cccctgagat
    16621 cttcgagccg ctcatggaga acccgtacat ccacaccacc acgttcggcg gcaacccgat
    16681 ggccacgtcc gccgtgatcg gcgccctgca cgcgaccctg gacgaggaga tcccccgcca
    16741 ggccgccgag aagggcgcct ggctcatgga gcgcttcgag gagctccgcg agcggcatcc
    16801 ggacctgctg cacgaagtcc gcggcttggg cctgctcatc ggcctcgagt actgcagcga
    16861 agaggccggc tacgccgtgg ccaccggcct cttcggcgag ggggtgctca ccggcggcac
    16921 gctcttcagc gccaagacct tccgcatcga gccgccggcc acgctgacgc agcacgagat
    16981 ggacgagatc gtggcccgcc tcgacctcgt catggggcgc gtgcaggcgc aactgggcgc
    17041 cggggagctc gcggcgcact gatctccgcg gcgggagacg ccgcgagacg cgcgtacgga
    17101 ccacgggccg gcgccctcag ggcgccggcc cgacggcgtc aggctcccgg acgacgccgg
    17161 tgccgcttca ggcccgctcg gcgttgccgt ccaccaggag cgcctggccg gagatcttcg
    17221 cgccggccgc cgaggcggcg aacaggatga ggctcgccac gtcgtcggcg gtcaccatcg
    17281 tcctcaggga gacggcctcg acgcgctcgg cggagaccgc ctcgggcgtg aggccgcgat
    17341 gctgcgcctc cagcgcgatg acgcgccgga tgcgctcgcc ctccacgtcg ccggggcaga
    17401 ccgcgttgac gcggataccg tacgggccga gctccatggc cagcgtcttc acgaggccga
    17461 tgagccccca cttggtggcg acgtagggag cacgatacgg caggcccatc gtcccggcgt
    17521 tggacgacat gacggcgatc gacccgccgc cggcggcttt gaggtgcggc accgccgcgc
    17581 ggcagcagag gaacgtgccg gtgaggttca cgtccagcgt ccgccgccag ccctccacgt
    17641 ccatgtcctc gaccggcgcg gccgggccgg cgacgccggc gttgctcacg agcacgtcga
    17701 ggccgccgag cctggcggtg gcggcggcca ccacctcatc cacctgctcc gggtccgcca
    17761 cgtcggcgac ctgtcccgtg acggcagggt gaccggtgag gtacgcgcgc agcgcgtcct
    17821 cgtccgcatc gcagatgtgg acctcggcgc cggccgcggc gaaccgcgcc gcgatcgtgg
    17881 cgcctatccc gccggcggcg gccgtcacca gcacgcgcct gccggcgatg tcgtcgagcc
    17941 tcacaggacc tcctcggggc tgctgccggc ggcatcgccg cctgcaggga gcacaacggg
    18001 agcgagggcc gggatgcccg cagacgtccc ggccctcgcc gtgtccgtgc ggcggcctca
    18061 gctgcgccgg gccgccccgc gcctcaccag tcggagaacc cggtgttgta ccagatcttc
    18121 ttggtgccct tcttggcgcg cttctcccac ttcctggtct tgggctcgcg gtcgatgcgc
    18181 gcccggacct cgtcggccac ctgcttgatc ttggccttct ggtcgtcgct cagcgcgttg
    18241 acgccgtcga cgtggtcctt gacgcggtcg aggttcgtgg tcgcggtgta ccagaagccc
    18301 cagtcgtcgg cgagccgctc cgcgatgaac ttgacgttga ccttcttggt gtcgtcctca
    18361 ccgaagtcgg ccacggtgaa gaggtactcg atgtccttga ggtccttgtc gttgatctcc
    18421 cagatctgca acttctgcag caggatgtcg gcgagcgaga cgctccaggg gtcgagctcg
    18481 agacgccccg catagttgat ctcgtggcag tagtcgagct tgtcgaagaa gatgtcgatc
    18541 atcgggacct tgccgccgaa gtagatctgg cgggtctgcc cggtcatggc catggtctgc
    18601 aggtcgttct cgtacccgag cgactccatc agcttggcca tgcccttgcg gcccttgccg
    18661 tagacggcgt agtcgatgtc ggtgaagtac cgctcgccca gacgctcgag cttggcgtag
    18721 aggtcgacct ggtccgggaa gtagtagtgg agcgcgaggg ggcccatgac gcgcatgatg
    18781 atgccctgct gctgcgcggc ctctgcgatc cgcttgccct cctcgaggaa gacctcaggc
    18841 ttgggatagc ggtcgagcgg gatgtccttc ggcaggatga actgtcccat atgtgtctat
    18901 cgcccccttc cgggttcggc gtcgcgttcg aagagtggag tctacgctcg gctcacccgg
    18961 aggtgaagac gtagtccttg atcttcttct tgtcgagcat gacgatgacg cccttgagga
    19021 tgccctccga gtactcgctg cccgggttga tgagcagcgt gcggccggcc ttctgcgcgc
    19081 cgcgcgactc gtggatgtgg ccgtgcaggc cgagcagggg ctggttctcc tcgatgaccc
    19141 gcttggcgcc caggctgccg gcgtggatct tctcgtcggc ggccatggtg aggttctcgt
    19201 ccagcttcgg gcacaggtcg agcgagtacc cgtacggcgg cacgtggaag ttgtagatcg
    19261 ccgtgtcgtg gttcttgacc ttggcgcaca gctcgcgcag gtgcttctcg aggtcttcct
    19321 cgctcatctc gcgcggcgtg tcccacggcg t
//
LOCUS       DASUMX010000269         5889 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_387411, whole genome shotgun sequence.
ACCESSION   DASUMX010000269 DASUMX010000000
VERSION     DASUMX010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 5889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5889
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387411"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="VFZ86_12885"
     CDS             complement(<1..321)
                     /locus_tag="VFZ86_12885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016353349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HEX5643213.1"
                     /translation="MSDLSLFATVPRGLESLLAGELRALGAAHVKQVRAGVAFSGSLE
                     TAYRACLWSRVAGRVLLPVASGTAADGDDLYKTAQSVDWSRHLAVDDTLAVDFTGVND
                     RIRDT"
     gene            complement(438..1583)
                     /locus_tag="VFZ86_12890"
     CDS             complement(438..1583)
                     /locus_tag="VFZ86_12890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5643214.1"
                     /translation="MKRPFSALLIVAVLTAVLVAAGPVLAWPGGSHAQSLDLQEDFNY
                     VGAAVKSMTDSTGHVVNYIDQGEEGWRTVVLIGGAGTGARAFELTEFVRDLREELGIR
                     VICVERNGIGMTEFEPAGADGEVIPADAGDEALALADPVKTREMYARDVLEVLDKEGV
                     GKFSIIGISGGGPYTGELARQAGRRVRSIHLAVALYQSTPGGGYPDPAFLETFRGYII
                     NPMWWWDMTGTTADLIPGWQEAAYEEAAWAAFVRGQDADPHAVAMDYTVYKMTPPDVS
                     MVTAPVYTYYGELDTTVPLVNRDRWIAAYSSAKSIKQRNYPDGVHDVQYRHYDQILLD
                     VAGYGDYLVVAWKGKTRVIPESKWAWYAAHGATLGIQAWVTAQPPES"
     gene            complement(1759..3750)
                     /gene="recQ"
                     /locus_tag="VFZ86_12895"
     CDS             complement(1759..3750)
                     /gene="recQ"
                     /locus_tag="VFZ86_12895"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HEX5643215.1"
                     /translation="MTPTSPAGPAAAPRSARALLREVFGFAEYRPHQEEIVDHLVAGG
                     DAFVLMPTGGGKSLCYQLPAMVRAGTGVVVSPLISLMKDQVDALLAAGVSAAAYNSSL
                     EAGEARGVLRALHAGELDLLYVAPETLMTEGFLERLRGLGEPGSGAAGSGAAGVALFA
                     IDEAHCVSQWGHDFRPEYVQLGRLRGLFPGVPIMACTATADPETRDDVRLRLGLADAA
                     VYVTGFDRPNIRYTVVEKREPLHQLLQFLAAHERESGIVYCLSRKRTEEVADKLRAHG
                     VEAAAYHAGLPADERRRVQDAFARDEVSVVVATVAFGMGIDKSDVRFVVHHDLPKTVE
                     SYYQETGRSGRDGLPADALLLFGLGDAAVVRSLIEGGGRGQNGDEGFERDPERVRIEI
                     HKLNSMVGYADGLSCRREALLGYFGEPYPAPCGNCDICLEPPETFDATEQARMALSCV
                     FRLWERDGFGYGVGYVIDVLRGSENEKILTRGHDALSTYGIGAELSRDHWQSLIRQLI
                     HRGYLAQDIARYSALRLTEAARPLLRGDETLIMAKPRVRVPSKKQRLRADRAGRAADL
                     AGMPVDEALFDELRALRKRLADEQNVPAYVVFSDATLAEMAARRPGTSAELLEVNGVG
                     QTKLERYGDAFLALVAERASGGSAPDGSAPDGSAPGGLF"
     gene            3824..4267
                     /locus_tag="VFZ86_12900"
     CDS             3824..4267
                     /locus_tag="VFZ86_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HEX5643216.1"
                     /translation="MKYSSATIGRVLVIRLEDGDVVHECIEEAARAEGIARAAVILLG
                     GARGGSRVVVGPEDGDARPVVPIERVLHDVHEMAGAGTIFPDESGRPVLHLHAAFGRD
                     DQVTAGCIRTGVRTWVVGEAVVIELAGSQATRRVEQSTGFEVLDA"
     gene            4530..>5889
                     /locus_tag="VFZ86_12905"
     CDS             4530..>5889
                     /locus_tag="VFZ86_12905"
                     /inference="COORDINATES: protein motif:HMM:NF012340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin like domain-containing protein"
                     /protein_id="HEX5643217.1"
                     /translation="MTGPCRRRSRQGRGALLAVLGALLVAAAALPASAAAAPWTGRTF
                     PSSYDLRDRGRVSPIGKQDNHGTCWVFAAMGSLESNLLPEYRLDLSENHLANFQASRL
                     RFEGRAPSNISTAYVARWEGPVLERDDPYPRPGDSPEGLRAVRHVQEVLFLPRRDGAQ
                     ENAAIKWAVMQYGGVDATMAYQHPDYNRATSAYYSRDTVLDHHVGIVGWDDAYPAGRF
                     LRRPPGPGAFLIKNSWGATFGQRGYFWISYHDRSLGAQLAVFNGVESARNHDAIYQHD
                     ALGWSKSVGFNDRTAWFAARYTSGGDGSVTAASFYTAKPGATYEVRVAPSLAEIAAAP
                     VAGAGVVDVGGYHTVPLSTPVPVTTSADFVVAVRLRTPGSLTPIPVEHPTRLLSPRPA
                     VGRSFISRDGVTWKDLRTRSGLGSSDVCLKAFVDSAGARDDAPPRVRLEPASARPGAG
                     TRV"
BASE COUNT          832 a         2124 c         2107 g          826 t
ORIGIN      
        1 cgtgtcccgg atcctgtcgt tgacgccggt gaagtcgacc gccagcgtgt cgtccaccgc
       61 gaggtggcgc gaccagtcga cggactgcgc ggtcttgtag aggtcgtcgc cgtcggcggc
      121 cgtcccgctc gcgaccggca gcagcacccg gccggcgacc cgcgaccaga ggcacgcgcg
      181 gtacgccgtc tcgagcgagc ccgagaacgc gacgccggcg cggacctgct tcacgtgtgc
      241 cgcgccgagc gcgcgcagct cgccggcgag cagcgactcg agaccgcgcg ggacggtggc
      301 gaagagggag aggtcgctca tccggcaagg ctaccacgcc gggacaccgc gccggcctgc
      361 gacgacgtga ccacggagac gccgaagggc ggggcgtgca ggcacgcccc gcccttcatc
      421 aagcgcacgt ccgccgctca gctctccggc ggttgcgcag tcacccacgc ctggatgccc
      481 agcgtcgcac cgtgggcggc gtaccacgcc cacttgctct ccgggatcac ccgcgtcttg
      541 cccttccagg cgacgaccag gtagtcgccg tacccggcca cgtccagcag gatctggtcg
      601 tagtgccggt actgcacgtc gtgcacgccg tccgggtagt tgcgctgctt gatggacttc
      661 gcgctcgagt acgccgcgat ccagcggtcg cggttgacca gcggcacggt cgtgtccagt
      721 tcgccgtagt aggtgtacac cggagccgtc accatggaga cgtccggcgg ggtcatcttg
      781 tagacggtgt agtccatggc caccgcgtgc gggtccgcat cctggccgcg cacgaacgcc
      841 gcccaggccg cctcctcgta ggccgcttcc tgccagcccg ggatgaggtc ggcggtggtg
      901 ccggtcatgt cccaccacca catcgggttg atgatgtaac ccctgaaggt ctcgaggaac
      961 gccgggtccg ggtacccgcc gcccggcgtc gactggtaga gggcgacggc gaggtggatg
     1021 gagcgcacgc gccgcccggc ctgccgcgcc agctcgccgg tgtaggggcc gccgccggag
     1081 atgccgatga tgctgaactt cccgacgccc tccttgtcca ggacctccag gacgtcacgg
     1141 gcgtacatct cgcgcgtctt gacggggtcg gccagcgcca gggcttcgtc gccggcgtcc
     1201 gccgggatca cctcgccgtc ggcgccggcc ggctcgaact ccgtcatgcc gatcccgttg
     1261 cgctccacgc agatcacgcg gatgccgagt tcctcgcgca ggtcgcgcac gaactcggtg
     1321 agctcgaagg cgcgggcgcc ggtgccggcc cctccgatga ggaccacggt gcgccagccc
     1381 tcctcaccct ggtcgatgta gttgaccacg tggccggtgg agtccgtcat cgacttcacg
     1441 gccgcgccga cgtagttgaa gtcctcctgc aggtccagcg actgggcatg ggacccgccc
     1501 ggccacgcca ggaccgggcc cgctgcgacg agcacagcgg tgaggacggc gacgatgagt
     1561 agagcggaga acgggcgttt catccggacc tcctgtgagt acgcggcgga tcgccgcggg
     1621 ccacccaccc gggtggttac gtcacagtac ccatgccggg aaggcggtgt caacgtatgt
     1681 tgggatgacc cgatgcggta tgttccgtcg acgctcggcc acgcgcgccg agcctggcgg
     1741 aagccgcagg cgcggcggct agaacagtcc cccgggggcc gagccgtcgg gagccgagcc
     1801 gtcaggggcc gagccgccgg aggcgcgctc ggcgaccaga gccaggaacg cgtcgccgta
     1861 ccgctcgagc ttggtctggc cgacgccgtt gacctcgagc agttccgcag acgttcccgg
     1921 ccgccgcgcg gccatctcgg ccagggtcgc gtcgctgaag accacgtagg ccggcacgtt
     1981 ctgctcatcc gccagccgct tgcgcagcgc gcgcaactcg tcgaacagcg cctcgtcgac
     2041 gggcatgccg gcgagatcgg ctgcccgtcc ggcgcgatcc gcgcgcagcc gctgcttctt
     2101 gctcggcacg cggacccgcg gcttggccat gatcagggtc tcgtcgccgc gcaggagcgg
     2161 ccgcgccgcc tcggtgagcc ggagcgccga gtagcgcgcg atgtcctggg cgaggtagcc
     2221 acggtggatg agctggcgga tgaggctctg ccagtggtcg cggctgagct cggcgccgat
     2281 gccgtaggtg ctgagcgcgt cgtggccgcg ggtgaggatc ttctcgttct ccgagccgcg
     2341 caggacgtcg atgacgtagc cgacgccgta cccgaacccg tcgcgctccc acagccggaa
     2401 gacgcagctg agcgccatgc gcgcctgctc ggtggcgtcg aaggtctccg gcggttcgag
     2461 gcagatgtcg cagttgccgc acggcgcggg gtacggctcg ccgaagtagc cgagcagggc
     2521 ctctcgccgg cagctcaggc cgtcggcgta gccgaccatc gagttgagct tgtggatctc
     2581 gatgcgcacg cgctccgggt cgcgctcgaa cccctcgtcg ccgttctgcc cgcggccgcc
     2641 gccctcgatg aggctgcgca ccacggcggc gtcgccgagg ccgaacagca gcagggcatc
     2701 ggcgggcagg ccgtcgcggc cggagcggcc cgtttcctgg tagtagctct cgaccgtctt
     2761 ggggaggtcg tggtgcacga cgaagcgcac gtcgctcttg tcgatgccca tgccgaaggc
     2821 gaccgtcgcc acgaccacgc tcacctcgtc gcgcgcgaag gcgtcctgga cgcgccgccg
     2881 ctcgtccgcc ggcaggccgg cgtggtacgc ggcggcctcg acgccgtgcg cgcgcagctt
     2941 gtcggccacc tcctcggtac gcttgcgcga gaggcagtag acgatgcccg actcgcgctc
     3001 gtgcgcggcg aggaactgca gcagctggtg cagcggctcg cgcttctcga ccaccgtgta
     3061 gcggatgttg ggccggtcga agccggtgac gtacaccgcg gcgtcggcga gcccaagacg
     3121 gaggcgcacg tcgtctcgcg tctcggggtc ggcagtggcc gtgcaggcca tgatggggac
     3181 accggggaag aggccgcgca gccggccaag ctggacgtac tcggggcgga agtcgtggcc
     3241 ccactggctg acgcagtgcg cctcgtcgat ggcgaacagg gcgacgccgg ccgcgccgct
     3301 cccggccgcg ccgctcccgg gctcgcccag cccgcgcagc cgctcgagga atccctcggt
     3361 catgagcgtc tcgggcgcca cgtacagcag gtcgagctcg ccggcgtgca gggcgcgcag
     3421 cacgccgcgc gcctcgccgg cctcgagcga cgagttgtag gcggcggcac tgacgccggc
     3481 cgccagcagt gcgtccacct ggtccttcat cagcgagatg agaggcgaga cgacgacgcc
     3541 ggtgccggcg cggaccatgg cgggcagctg gtagcagagg gacttgccgc cgccggtggg
     3601 catgagcacg aacgcgtcgc cgccggcgac caggtggtcg acgatctcct cctggtgcgg
     3661 gcggtactcg gcgaagccga agacctcgcg cagcagggcg cgagcagagc gcggcgcggc
     3721 cgcgggaccg gcgggggagg tcggagtcac gcgttcgagt ctagcaaggt tgacggacgg
     3781 gagcgcggcg ccgggtagga tgcccttcgc aaggaggtca ctgatgaagt acagctcagc
     3841 cacgatcggc cgcgtgctcg tcatccgcct ggaggacggc gacgtggtgc acgagtgcat
     3901 cgaggaggcg gcgcgagccg aaggcatcgc gcgcgccgcc gtcatcctgc tcggcggcgc
     3961 ccgcggcggc agccgcgtgg tcgtcgggcc cgaggacggc gacgcccggc ccgtcgtgcc
     4021 catcgaacga gtgctgcacg acgtgcacga gatggcgggc gccgggacga tcttcccgga
     4081 cgagagcggc cggccggtgc tgcacctgca cgcagccttc ggccgcgacg accaggtgac
     4141 cgccggctgc atccgcaccg gcgtcaggac gtgggtcgtc ggcgaggccg tggtcatcga
     4201 gctcgccggg agccaagcga cccggcgcgt cgagcagtcc acgggattcg aggtgctcga
     4261 cgcctgagcg gcgccgaccg gcgccgatcg cgccaccaaa gaagccgggg cgggacccag
     4321 agggcccgcc ccggctttct cgcccggagt cggtgtccgg cgccggctcg atcccgccgg
     4381 ggatcgcgcc aggtccgtcg ccgcgtccga gaaacctcga tcggcggtac agccgccgat
     4441 tcggcgcatt gtacacgttt tgtctactga ggcaatccct ttagtcaatt gtgatgcgtc
     4501 gcacatgttc agtgtgtatc cttcgtgcca tgaccggacc gtgcagacga cgttcgcggc
     4561 agggccgtgg ggcactcctc gccgtcctgg gcgcgctcct ggtcgcggcg gcggcgctgc
     4621 cggcgtcagc cgccgccgcc ccctggaccg ggcgcacgtt cccgtcgagc tacgacctgc
     4681 gcgaccgcgg acgggtgtcc cccatcggca agcaggacaa ccacggcacc tgctgggtct
     4741 tcgccgccat ggggtcgctg gaatcgaacc tcctgcccga gtaccgcctc gacctctcgg
     4801 agaaccacct ggcgaacttc caggcgtcgc gcctgcgctt cgaggggcgc gcgccgagca
     4861 acatctcgac cgcctacgtc gcccggtggg aggggccggt cctcgagcgc gacgacccgt
     4921 acccgcgtcc gggagactcg cccgagggac tgcgcgccgt gcgtcacgtg caggaagtgc
     4981 tcttcctgcc gcgccgcgac ggcgcccagg agaacgcggc gatcaagtgg gcggtcatgc
     5041 agtacggcgg cgtggacgcg accatggcct accagcaccc cgactacaac cgcgcgacga
     5101 gcgcctacta ctcgcgggac accgtgctcg accaccacgt cgggatcgtg ggatgggacg
     5161 acgcctatcc ggcgggccgc ttcctgcgcc gcccgccggg ccccggcgcg tttctgatca
     5221 agaacagctg gggtgcgacc ttcggccagc gaggctactt ctggatctcc taccacgacc
     5281 gcagcctcgg cgcccagctc gccgtgttca acggcgtcga gagcgctcgc aaccacgacg
     5341 ccatctacca gcacgacgcc ctcggctggt cgaagagcgt cggcttcaac gaccgcaccg
     5401 cctggttcgc cgcgcgctac acgagcggcg gcgacggcag cgtgaccgcc gcgagcttct
     5461 acacggccaa gccgggcgcg acctacgaag tccgggtcgc gccgtcgctg gccgagatcg
     5521 ccgcggcgcc ggtcgccggc gccggcgtcg tggacgtcgg cggctaccac acggtgccgc
     5581 tgtcgacacc cgtccccgtg accaccagcg ccgacttcgt cgtcgccgta cgcctgcgga
     5641 cccccggctc cctgacgccg atcccggtcg agcacccgac caggcttctc tcgccacgcc
     5701 ccgccgtcgg gagatccttc atcagtcgcg acggagtgac gtggaaggac ctccgcacgc
     5761 gctccggact cggctcgtcc gacgtctgcc tcaaggcgtt cgtcgattcg gcgggcgcgc
     5821 gagacgatgc tccgccccgc gtgcgcctcg agcccgcgtc cgcgcgcccg ggtgccggaa
     5881 cgcgcgtcc
//
LOCUS       DASUMX010000270         5662 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1393271, whole genome shotgun sequence.
ACCESSION   DASUMX010000270 DASUMX010000000
VERSION     DASUMX010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 5662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5662
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1393271"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..1237)
                     /locus_tag="VFZ86_12910"
     CDS             complement(29..1237)
                     /locus_tag="VFZ86_12910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643218.1"
                     /translation="MRHRHLATLVAAFALLTLLVAAPIAGATGGSSGPSLSWTKVYGD
                     DPSGWTGRAGLQAVQLRGDFFVMGGRTPDPSQFNPFASILWNDVWKSDDLGRSWRALG
                     TAPWAARGYFQAVTKDGAMFVLGGQNLSAGPGQPSQFFNDVWKSYDGKNWKQLTAAAP
                     WKGRAGLSAIVFGDHIYVMGGSNGDDVAIGGSGRELFNDVWRSRDGRSWQLVTADAAW
                     SPRAGAAVVKKDGYLYLLGGEDGFLCTVGPAGLQCPYFNDVWRSRNGATWDLVTPSAA
                     WSPRPGHQAQVLYGAIVVFGGFGWPSLADPTQPPGPFNPFLPGHPTDQWSSRDGKNWT
                     LLPGSPWNAAGPADIKYDFDSLKVRTGLFGLRESIYTFGGDREISFVNPDPSLVDDDV
                     WRAMPRLWSW"
     gene            1538..1915
                     /locus_tag="VFZ86_12915"
     CDS             1538..1915
                     /locus_tag="VFZ86_12915"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX5643219.1"
                     /translation="MIDETRIGDWLAAYRHAWSTDDPAEIARLFKDDVRYFTAPYREP
                     LEGVGAVTAYWIGEDEGRIPWSFEEQVLAQEGDLFIIRAVTTYPEGGSRDDDPAQFHN
                     LWLVTLDADGRAREFVEYYMLVE"
     gene            2003..3250
                     /gene="trpB"
                     /locus_tag="VFZ86_12920"
     CDS             2003..3250
                     /gene="trpB"
                     /locus_tag="VFZ86_12920"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013042648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEX5643220.1"
                     /translation="MAEQATVHEAEAEHAARRRAVPDEAGFFGKYGGQYLPPQLEAPF
                     AEIVRAYHEIENDAAFIAELRYLRKHFQGRPTPVYHARTLSLANGGAQIYLKREDLNH
                     TGAHKLNHCMGEGLLAKYMGKKKLIAETGAGQHGVALATAAAHFGLACEIHMGVVDIE
                     KQAPNVSRMQLLGATVVPVTHGLQTLKEAVDSAFEAYLADYENSIYCIGSVVGPHPFP
                     LMVRDFQKVVGMEAREQFVEMTGNLPDVVEACVGGGSNAMGIFSGFLDDPVELIGVEP
                     LGHGEGVGEHAATMTYGTEGVIHGFRCYLLQDEQGEPAPVYSCASGLDYPGVGPEHSY
                     LKDSGRVQYVTCSDDACRDAFFALSRREGIIPALESAHAVAHALVKAREMKTGTILVN
                     LSGRGDKDMDYVIEHWGIGDDEG"
     gene            3302..4240
                     /locus_tag="VFZ86_12925"
     CDS             3302..4240
                     /locus_tag="VFZ86_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEX5643221.1"
                     /translation="MPQLGGGLFLADGGIETSLIVHEGLALTDFAAFPLLRTGEGRAA
                     LRRYYRPYAEIARRHGVGLVLESATWRASAGWGAPLGFTAEMLEEIDRKSIRLLEDVR
                     DEFEGGPTRIVVSGCIGPRRDGYDGAEAMTETEAEAYHAEQIAVFADTPADMVAALTL
                     TNVEEALGITRAAARAGMPVAVSFTVETDGRLASGQSLRHAIEAVDAATSAYPCYYMI
                     NCAHPEHFGRALAEGGPWAARIRGLRANASRLSHAELDEAPAPDAGDPEELGREYVEL
                     KRRLPQLTVLGGCCGTDQRHVGHIAAACAPLFQDGA"
     gene            4230..5417
                     /locus_tag="VFZ86_12930"
     CDS             4230..5417
                     /locus_tag="VFZ86_12930"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HEX5643222.1"
                     /translation="MARDAGGRPDARAGPAAAFALDAEAGREIVARRLRAQRLTGAGF
                     ARPEDVVGRLAAVQAQDYGPAKWSLGARVAGATDAQVEGAFASGAILRTHVLRPTWHF
                     VLPADIRWLLTATAPRIRARDARRYAQLGLDGDTLRRSAAALVAALRGGNRLTRREAA
                     AVLTAAGVGVEGQRLPYLLMAAELDALICSGPRSGKQHTHMLLEERAPGARDLPRREA
                     LAELARRFFAGHGPATAKDFAVWATLTLAEARAAIEAAGPGLRAEDLGGLRFWASATG
                     PGSGGERLAAPRRPLVHLVQGYDEIVMGYSETKPLLARPGSPWKPATPPVFRLVVLLD
                     GGVAGFWRPRPKSDRVIVEVSLLEAFGDPAAAALEAEAARYGAFLGLHADVRLVGSPQ
                     RAR"
     gene            complement(5462..>5662)
                     /locus_tag="VFZ86_12935"
     CDS             complement(5462..>5662)
                     /locus_tag="VFZ86_12935"
                     /inference="COORDINATES: protein motif:HMM:NF017273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEX5643223.1"
                     /translation="HMPAGIMVILVVYSLRGISLGEAPHGLPEALAVIATVVLHLVKG
                     NAVASIIGGTAVYVLLVNLVFT"
BASE COUNT          817 a         1963 c         2115 g          767 t
ORIGIN      
        1 tcttggctca gtttggctca gtcccggtct accaggacca caggcgcggc atcgcgcgcc
       61 agacgtcgtc gtcgacgagt gacggatccg ggttcacgaa cgagatctcg cggtcgccgc
      121 cgaacgtgta gatcgactcg cgcagcccga agaggccggt cctgaccttg agcgagtcga
      181 agtcgtactt gatatcggcc gggcctgcgg cgttccaggg gctgcccggc agcagcgtcc
      241 agttcttccc gtcgcggctg gaccactggt cggtcggatg gccggggagg aacgggttga
      301 agggtcccgg cggctgcgtc gggtccgcga gcgacggcca gccgaagccg ccgaacacga
      361 cgatggcgcc gtagagcacc tgggcctggt gtccggggcg cggactccag gccgcggacg
      421 gcgtgaccag atcccacgtg gcgccgttgc gggagcgcca cacgtcgttg aagtaggggc
      481 actgcaggcc ggccggaccg accgtgcaga ggaagccgtc ctcgccgccg aggaggtaca
      541 ggtacccgtc cttcttgacg acggccgctc cggcgcgcgg ggaccaggcg gcgtcggcgg
      601 tgaccagctg ccacgacctg ccgtcgcggg agcgccagac gtcgttgaag agctcgcgac
      661 cgctgccgcc gatggcgacg tcgtcgccgt tgctgccgcc catgacgtag atgtggtcgc
      721 cgaacacgat ggcgctgagg ccggcccggc ccttccacgg tgcggcggcg gtcagctgct
      781 tccagttctt gccgtcgtac gacttccaga cgtcgttgaa gaactgggac ggctgccccg
      841 gtcccgcgga caggttctgg ccgccgagca cgaacatggc gccgtccttg gtcaccgcct
      901 ggaagtagcc gcgggcggcc cacggcgccg tgccgagcgc ccgccacgag cgcccgaggt
      961 cgtcgctctt ccacacgtcg ttccacagga tgctggcgaa cgggttgaac tgggacgggt
     1021 ccggggtgcg gccgcccatc acgaagaagt cgccacgcag ctggacggcc tgcaggccgg
     1081 cacggccggt ccagcccgag ggatcgtcgc cgtagacctt ggtccacgac agcgacgggc
     1141 cggagctgcc gccggtcgct cccgcgatgg gggcggcgac gagcagggtg agcagggcga
     1201 acgccgcgac gagcgtcgcg aggtgacggt gtctcatgtc tctctccttg gaaaggtggt
     1261 cgcgaggtcc tcccagccac ctcgacgacg aatgagcttg tgtacccggg gctccgccgc
     1321 ttcagccacg actcgggctg ccgcgcatcg gcggcgttgt ccacggtgac aacgcacggc
     1381 ggcccaaggg gctcgggagt cgcgccgccc gtctcgcctg cgcccgagcc gaggccgccg
     1441 ctcgtcgacg gttgggtgcg gcgtctgcgc gggcaagtcc tcggggaggc gacgacgagc
     1501 tcgcccgtca cgggacgcgg accacgagga ggctgcgatg atcgacgaga ccaggatcgg
     1561 cgactggctg gcggcgtacc ggcacgcctg gtccaccgac gaccccgcgg agatcgcccg
     1621 gctgttcaag gacgacgtgc gctacttcac cgcgccgtat cgcgaaccgc tggagggcgt
     1681 cggggcggtg accgcctact ggatcggcga ggacgagggc aggatcccct ggtcgttcga
     1741 ggagcaggtc ctggcgcagg agggcgacct cttcatcatc cgtgccgtga ccacctatcc
     1801 cgagggtggg agtcgcgacg acgacccagc gcaattccac aacctctggc ttgtgacgct
     1861 ggacgccgac ggacgcgcgc gcgagttcgt cgagtactac atgctcgtgg agtgagccgg
     1921 aggacggcgg cgcgcaggcc gccgccgggt cgcgtagact caccggcggt ccattcacgc
     1981 gcgagcgcac gaggtgagca cgatggcgga gcaggcgacg gtccacgagg cggaggcaga
     2041 gcacgcggcg aggcggcggg cggtgcccga cgaggccggg ttcttcggca agtacggcgg
     2101 gcagtacctg ccgccccagc tcgaggcgcc cttcgccgag atcgtccgcg cgtaccacga
     2161 gatcgagaac gacgccgcct tcatcgcgga gctccgttac ctgcgcaagc acttccaggg
     2221 acggccgacg ccggtgtacc acgcgcgcac cctgtcgctc gccaacggcg gcgcgcagat
     2281 ctacctcaag cgcgaggacc tcaaccacac gggcgcgcac aagctcaacc actgcatggg
     2341 cgaggggctg ctggccaagt acatgggcaa gaagaagctc atcgccgaga ccggcgccgg
     2401 ccagcacggc gtcgccctcg ccaccgccgc ggcgcacttc ggcctcgcgt gcgagatcca
     2461 catgggcgtg gtggacatcg agaagcaggc gcccaacgtg agccgtatgc agctcctcgg
     2521 cgccaccgtc gtcccggtca cgcacgggct gcagaccctc aaggaggccg tggactcggc
     2581 gttcgaggcc taccttgcgg actacgagaa ctccatctac tgcatcggct ccgtcgtcgg
     2641 ccctcatccc ttcccgctca tggtgcgcga cttccagaag gtcgtgggca tggaggcgcg
     2701 cgagcagttc gtggagatga ccggcaatct gccggacgtg gtggaggcgt gcgtaggcgg
     2761 cgggtccaac gcgatgggaa tcttctccgg cttcctcgac gacccggtgg agctcatcgg
     2821 cgtcgagccc ctggggcacg gcgagggcgt gggcgagcac gcggcgacga tgacgtacgg
     2881 gaccgaaggc gtcatccacg gcttccgctg ctacctgctc caggacgagc agggggagcc
     2941 ggcgccggtg tactcctgcg ccagcgggct ggactacccc ggtgtcgggc ccgagcacag
     3001 ctacctcaag gactcgggcc gcgtgcagta cgtgacctgc tccgacgacg cctgccgcga
     3061 cgcgttcttc gcgctcagcc gcagggaggg catcatcccg gcgctggaga gcgcgcacgc
     3121 ggtggcgcac gcgctcgtca aggcccggga gatgaagacg ggcaccatcc tggtcaacct
     3181 cagcggacgc ggcgacaagg acatggacta cgtgatcgag cactggggca tcggcgacga
     3241 cgagggctga tccgtgtgtc cgcaccgaca gggagggcgc gcggcacgct accgggacgc
     3301 gttgccgcag ctgggcggcg gtctcttcct cgctgacggc ggcatcgaga cgagcctcat
     3361 cgtccatgag gggctggcgc tcacggactt cgccgcgttc ccgctgctgc gcacggggga
     3421 gggacgggct gctctgcgcc gctactaccg cccctacgcg gagatcgccc ggcggcacgg
     3481 cgtggggctg gtgctcgaga gcgcgacgtg gcgggcgagc gccggctggg gtgccccgct
     3541 cggcttcacc gcggagatgc tggaggagat cgaccggaag tcgatccgtc tgctcgagga
     3601 cgtacgcgac gagttcgaag gcgggccgac gcggatcgtc gtcagcgggt gcatcggccc
     3661 gcggcgcgat ggctacgatg gcgctgaggc gatgaccgag accgaggccg aggcgtatca
     3721 cgccgagcag atagcggtct tcgccgacac cccggcggac atggtcgccg ccctgacgct
     3781 gacgaacgtc gaggaggccc tgggcatcac gcgggcggcc gctcgggccg gcatgccggt
     3841 cgccgtctcg ttcaccgtcg agaccgacgg tcgcctcgcg tcgggtcagt ccctgcggca
     3901 cgccatcgaa gcggtcgatg cagccacgtc cgcctatccg tgctactaca tgatcaactg
     3961 cgcccacccg gagcatttcg gccgtgcgct cgcggagggt ggcccctggg ccgcgaggat
     4021 ccgcggcctg cgcgcgaacg cctcacgcct gagccacgcc gagctcgacg aggcgccggc
     4081 gcctgacgcg ggggaccccg aggagctcgg acgcgagtac gtcgagctca agcggcggct
     4141 accgcagctg accgtgctgg gcgggtgctg cggcaccgac cagcgtcacg tcggccacat
     4201 cgcggcggcc tgcgcgccgc tgttccaaga tggcgcgtga cgcaggtggg cggcccgatg
     4261 cccgggccgg cccggccgcg gcgttcgcgc tggacgccga ggcgggcagg gagatcgtcg
     4321 cgcgccggct gcgcgcacag cgcctgaccg gcgccgggtt cgcgcgtccg gaggacgtcg
     4381 tcggccggct tgcggcggtc caggcccagg actacgggcc ggccaagtgg tcgctgggcg
     4441 cgcgtgtggc cggcgcgacc gacgcccagg tggagggcgc cttcgcctcc ggcgcgatcc
     4501 tgcgcaccca cgtgctgcgg ccgacctggc acttcgtcct gccggccgat atccgctggc
     4561 tgctgacggc gacggcgccg cggatcaggg ctcgtgacgc gcgccgctac gcccagctcg
     4621 gcctcgacgg cgacacgctg cggcggagcg ccgcggctct cgtagcggcc ctgcgcggcg
     4681 gcaaccggct gacccggcgc gaggcggcgg cggtcctcac cgccgccggc gtcggcgtcg
     4741 aggggcagcg gctgccgtat ctcctgatgg cggcggagct ggacgcgctc atctgcagcg
     4801 gcccgcggag cggcaagcag cacacgcaca tgctgctgga ggagcgagcg cccggcgcgc
     4861 gcgacctgcc gcgccgcgag gcgctcgcgg agctggcgcg gcgcttcttc gccggccacg
     4921 ggccggcgac cgcgaaggac ttcgccgtct gggcgacgct gaccctggcc gaggcgcgcg
     4981 ccgcgatcga ggcggcgggg ccggggctgc gggccgagga cctgggcggc ctgcgcttct
     5041 gggcgtccgc gaccgggccg gggagtgggg gagagcgcct cgcggcgccg cggcgaccgc
     5101 tggtccacct cgtccagggc tacgacgaga tcgtcatggg ctacagcgag accaagccgc
     5161 tgctggcccg gcccggatcg ccctggaaac ccgcgacgcc gccggtcttc cgcctcgtcg
     5221 tcctgctgga cggcggcgtc gccggcttct ggcgcccgcg cccgaagagc gatcgcgtga
     5281 tcgtcgaggt ctctctgctg gaggcgttcg gcgatcccgc cgcagcggcg ctcgaggccg
     5341 aggcggcgcg ctacggcgcg ttcctggggc tgcacgcgga cgtgaggctt gtgggctcgc
     5401 cgcagcgagc gcggtgacgg ccggccgggg atcgcagccc tcgccgccgg cgctccttca
     5461 gttacgtgaa cacgaggttg acgaggagca cgtagacggc ggtgccgccg atgatgctgg
     5521 ccacggcgtt gcccttcacg aggtgcagca ccaccgtggc gatgacggcg agcgcctccg
     5581 gtagcccgtg cggcgcctcg ccgagcgaga tcccgcgtag cgagtacacc acgaggatga
     5641 ccatgatgcc cgccggcatg tg
//
LOCUS       DASUMX010000271        12876 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1394190, whole genome shotgun sequence.
ACCESSION   DASUMX010000271 DASUMX010000000
VERSION     DASUMX010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 12876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12876
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1394190"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="VFZ86_12940"
     CDS             <1..598
                     /locus_tag="VFZ86_12940"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEX5643224.1"
                     /translation="DAQGNYIGTFWELLNPYSLLVGVTGLFLLVQHGAAWLAVKAEGE
                     LHARAWRWRQVTFWICAVLAVATSLATILTVSRASDNVFGRPAGWLFLAVFIAGVLYT
                     IWQLYKKDGALRSFIGSAIIIVGLAGIAAVGNFPEMVPARGTPESTSLTVERAASEDL
                     TLTVMLILAVIFVPVVLIYTALIYKTFWGKTKAGEADY"
     gene            812..2413
                     /locus_tag="VFZ86_12945"
     CDS             812..2413
                     /locus_tag="VFZ86_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020392486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX5643225.1"
                     /translation="MSASPPVREELDQDTKDLHSMGYAQELDRSMSKFSNFAVSFTII
                     SILSGCLTLYGYGLLHGGPPVMLWGWLLVGTLVIFAGMSLGEICSAFPTAGGLYYWSA
                     KLAPGNSAPIWSWFTGWFNLLGQVAVTAGISFGCAFSISAFFYILTGSDTWLEPYATI
                     LILAVVLFLQGLLNTFSVRLVALLNDVSVYWHLIGVAIIFVLLFWAPESGTHQSINFL
                     FGADGWNAFEGLSGFTFGLYVFLIGLLNAQYTFTGYDASAHVSEETINARISAPKGIV
                     NSIWISMVAGFILLLGVSMAIPTAFPVTINGVEYAGYDAIATDLVPWATIFEYATGHT
                     IGLLLVLIVIVAQFFCGMSSITANSRMVYAFARDGAVPFSDFWHHVSKRQRVPVRTAW
                     FGAIGAFILALPYLYNVVAYAAVTSIAVIGLYISYLTPVFLRRINPGVFEPGPWKLSK
                     TWGPIINWIAIIWVVFIVILLMLPQYSPGNTVETFNYAPVAVLVVVGGAGLWFAVSAR
                     KWFKGPKVQGTAEELAEIERDLNVI"
     gene            2778..3986
                     /locus_tag="VFZ86_12950"
     CDS             2778..3986
                     /locus_tag="VFZ86_12950"
                     /inference="COORDINATES: protein motif:HMM:NF013116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HEX5643226.1"
                     /translation="MKDPNLFEDLVAVSPGMEIWWDSSPVIFANWCDKMLAKADEADK
                     AALKAQFARMYNADDPMSQLFRGVTTNPPLSLAAFKDDPARWEKVAQKVLAENKDVDT
                     EGLFWLLYKEVVRQGSDMFLPLFEASGYKEGFLSGQVDPRSAFDGEAMLAQALEIHAV
                     NPNVMVKVPGTAQGYEVIEELTSRGIATNNTLTFVLSQLLDCAKSVMRGLEKAKANGV
                     DLSKWRSVITHMEARYGDLGGLREFGAEAGVEVTEADVRYAELAIFKKAYRTVKERGY
                     QSKMLSCSLRLGPTVDGEQRIWHLEEKAGASIVVTVPPPFIDEVINFPGADSIHFQAD
                     QIDVDPPADVMEKLLKIPYFARAYDEDGYTREEYNTHPALVKTHEAFSKATNEMVAFA
                     DGCLKGDCKA"
     gene            4031..5113
                     /locus_tag="VFZ86_12955"
     CDS             4031..5113
                     /locus_tag="VFZ86_12955"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEX5643227.1"
                     /translation="MTDIKDKINPYIWTAPEYNTGVLDMAWAHPELGRMMLNENPVPP
                     SDAVVAAITDMAKKGNRYPDRAYRLREKLGKLHGVAAENIALANGSSETIDAMMRIFL
                     QPGDEFLCSNPTFEMFPSRAGYCNAKTVQVPLRDEDLQYDVEGMLAAVNDKTKLILII
                     NPNNPTGIFIEDADLTRFAELGIPMCIDEAYLDYHPQVEAKVDFVKKYPHVFLSHTFS
                     KAFGLAGIRMGYMVGSPTVVDAFNLMYLPWNLSLMAIAAAEAILDTGEVKQKVEYNND
                     WMKKFADALSAKGLKPFEAHGNYMLVDASASGKTSAEIVQAANDTQQVIIKTIKPING
                     KEGYFRVTPGTPEESERFMAFIEAYF"
     gene            5210..7216
                     /gene="tkt"
                     /locus_tag="VFZ86_12960"
     CDS             5210..7216
                     /gene="tkt"
                     /locus_tag="VFZ86_12960"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002646683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEX5643228.1"
                     /translation="MSTVVSDPALQDRCIDTIRFLAVDGVQKANSGHPGMPMGAAAVA
                     YTLFTRHLRYDPADPQWPNRDRFVLSAGHASMLLYSLLHLTGYDLTLDDLKAFRQWGS
                     RTPGHPEYGHTAGVETTTGPLGQGVANAVGMAVAERFLAATYGDVMDHYTYVLAGDGC
                     MMEGISSEAASFAGHQQLGKLILLYDDNHITIDGSTDLAFTEDVCKRYEAYGWHVQKV
                     ADGNDVEAIDAAITAAKAETGKPSFIAVRTHIGCGSPNKQDKAAAHGAPLGADEVKLT
                     KETCAWPLDPEFHVPDEVREAFRAAGKQAAEAHAEWTRKYEAWRAADAARAASWDDAW
                     AHALPEGWDADLPVFTTEDKVATRAASGKVINAVAPHIPTLMGGSADLAPSNNTLIKD
                     APDQQAATPGGRNVRWGVRELAMAAMGNGLATHGGIRPYVATFFVFVDYMRPAMRLSA
                     LMGLPVIYVLTHDSIGVGEDGPTHQPVEHLAMLRATPGFIDLRPADANETVEAWRIAL
                     QTADAPVGLMLTRQNLPTIDREKYAPASGIARGAYVMDDASDGGAPDVILIASGSEVA
                     LALEAHERLVAEGVRSRLVNMASMRLFEQQDAGYRESVLPPACGARVAVEAGVTLGWD
                     RWIGDGGVVIGIDHFGASAPAGTLFQEFGFTADNVYARAKALLA"
     gene            7431..8252
                     /locus_tag="VFZ86_12965"
     CDS             7431..8252
                     /locus_tag="VFZ86_12965"
                     /inference="COORDINATES: protein motif:HMM:NF012669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HEX5643229.1"
                     /translation="MSSDREGQQMSPDVPSEVHEMIPAERRARIVELLEERRAVRVSV
                     LSETLGVSEMTIRRDLELLEQAGLLSRMHGGAILKRRMIEEPLYATNVRAHSEQKRRI
                     AKAAAGMIQPGETVFLSSGTTAAQVLSHVDPQLKARIVTHNVGALSTAQSSSLDIVLL
                     GGSYRQRSNTLEGPLAIEAVSTFHAGRFILGADGVSLEEGVTTPSIGLAGVERAMVRQ
                     TRGEIVVLADASKVGVIGDVAICTLDKVDTVIVDDAVDKDVRDEMERLGIVQIVV"
     gene            complement(8307..9323)
                     /locus_tag="VFZ86_12970"
     CDS             complement(8307..9323)
                     /locus_tag="VFZ86_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HEX5643230.1"
                     /translation="MDAAVIWDPAYAEHDTGEHPEGADRVSSTIDYLEGTDLWPRLTV
                     LTSPPPATEDDVLLVHTPAHLAMIKRAAQGRGTWIDPDTHVSPRSYETALLSAGGALL
                     AAELWGDGLAPFALIRPPGHHATPDRAMGFCLFNNVAIAAARLLQRGYERIAILDWDV
                     HHGNGTQAAFYAEPRILFGSAHQWPHYPGSGFFGECGEGAGEGSTVNVPLPAGADDAD
                     YAGVFDALFEPIVRQFAPQAIFVSAGQDIHRSDPLGEMRVTEAGFAQMALRCVRLAQE
                     LCEGRLAFVLEGGYDRVASARAVEAVLRAVADERAPEVSGGTERAAAAVARAREVQSA
                     YWDL"
     gene            complement(9389..10057)
                     /locus_tag="VFZ86_12975"
     CDS             complement(9389..10057)
                     /locus_tag="VFZ86_12975"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5643231.1"
                     /translation="MSPQDERRRSLARGEGLPEPLLLEDPMEALPPTAQRILAAAQRL
                     LAEGGFEALKLSAIAREAGESKASIGYHFGNKAGLVTALVDSLAHDSNRGLIEETAAT
                     PAGEARVHALIDGESRIAADAESFQSFFDVLPHALRDPDLRERVAALYDGYRETVLRC
                     LDAAGPDAPADLRPFAMLMIAIVDGLAIQHGLDPEGADVRAATELWERLARPYLTVGG
                     GSAA"
     gene            10188..11198
                     /gene="argF"
                     /locus_tag="VFZ86_12980"
     CDS             10188..11198
                     /gene="argF"
                     /locus_tag="VFZ86_12980"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF001986.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEX5643232.1"
                     /translation="MDTTLPALKKYRGRHFLTDLDYTREELEDLLKLAVGLKALWRKR
                     PLTPFLPGRHLAMIFEAASTRTRVSFENGFAELGGNALYLRPGEIHLPGRESIADTAR
                     TLSQYNAAIEIRSKKNETVNELAAWSAVPVINGMDHDRHPCQSMSDALTILEHAGTLA
                     GVTFAYVGDAAHPCNSLSTTLTKLGLNVRVGNAAGYEMAPELVELSTRFAAEAGGSFL
                     LTNDPLEAVAGADFVYTDCWWWTGQEDEYKDRVEAFRPFQVNPDLWSHAKPETKFMHC
                     LPAMRGEEVVDEIADGPMSIIFPQAQNRMHFQKALMLALIGIDELPADPDLQPIGKAL
                     LS"
     gene            11238..12686
                     /locus_tag="VFZ86_12985"
     CDS             11238..12686
                     /locus_tag="VFZ86_12985"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX5643233.1"
                     /translation="MATDLHDGAPGGTLTEKPKVFGLFSMVLFSVSAILVADTVGSSA
                     AMGVQGLGFWIILGLLFFIPYGFVTAELGSAWPDEGGIYVWVKTAFGPGWGTVTSWMY
                     WVNVALWAPSVFVLFIGAIEVAWGVDVPPLWEAVIVIAMVWAIVIVGILPLAWSKFVP
                     DLSAALKVIVLVGLGALGVAYVVQNGAANSFALSAWIPEWNTDSLSFLPIIIYSYMGF
                     ELMSSAGGNIKDPKRDVPKMIILAGAAILAVYMFATFGILASIKTEDVSIVTGITDAL
                     KLMFDEVLGGASWLFNIAIVALLFTFFGNMVTWSIGANQTIGATGLDETAPGVFGHVN
                     RRFGTPDYAFVLMGVIATAITILAYVLDPNQTSVFWTLFALSSIVFLIPYLLMFPALL
                     VLRHTHADQPRPYVIPGGKAGAWIATILCEAGIALTLFLFFYFPAEGVSKGVFWLITG
                     GGTIVSLLIGWWLYWHASHKPGGRNEAETVDA"
BASE COUNT         2074 a         4669 c         4139 g         1994 t
ORIGIN      
        1 ggacgcgcag ggcaactaca tcggcacctt ctgggagctc ctcaacccgt actccctcct
       61 cgtcggcgtc accggcctct tcctgctcgt gcagcacggg gccgcgtggc tggcggtcaa
      121 ggcggagggc gagctgcacg cacgcgcctg gcgctggcgc caggtcacgt tctggatctg
      181 cgccgtgctc gccgtggcga cgtcgctcgc gaccatcctc accgtctcac gcgcctccga
      241 caacgtgttc gggcggccgg ccggctggct gttcctcgcc gtcttcatcg ccggcgtgct
      301 ctacaccatc tggcagctgt acaagaagga cggcgcgctc aggtccttca tcggatcggc
      361 gatcatcatc gtcggcctgg ccggcatcgc cgcggtgggc aacttccccg agatggtgcc
      421 ggcgcgcggc acgcccgaga gcaccagtct cacggtcgaa cgcgcggcgt ccgaggacct
      481 cacgctgacg gtgatgctca tcctggcggt gatcttcgtg cccgtggtcc tgatctacac
      541 cgccctgatc tacaagacgt tctggggcaa gaccaaagca ggcgaggccg actactgagc
      601 gcaggcctga accgaatcac acatcgcctc ttgtcgacgg gcggcgggcc ggagggcccg
      661 ccgcccgttc tcttgctcac tatctaccaa attcgcaaac aacttcgcac atccccttga
      721 caggtcgcgg tgcttggtac tgaatggcgg tcaatacgcg tgagcgtagg ccagttgggc
      781 gagcaagggg gggttcatcg tgccagacga aatgtccgcg tcaccgccgg taagagaaga
      841 gttggatcag gacaccaagg atctgcactc gatgggctac gctcaggagc tcgaccgctc
      901 catgagcaag ttctccaact tcgccgtctc cttcaccatc atctccatcc tctcgggctg
      961 cctcacgctg tacggctacg gcctgctgca cggcggccct ccggtcatgc tctggggttg
     1021 gctgctcgtc ggcacgctcg tcatcttcgc cggcatgtcc ctcggcgaga tctgcagcgc
     1081 cttcccgacc gccggcggcc tgtactactg gtcggccaag ctcgcgcccg gcaacagcgc
     1141 tccgatctgg tcgtggttca ccggctggtt caacctgctc ggccaggtcg ccgtcaccgc
     1201 gggtatcagc ttcggctgcg ccttctccat ctcggcgttc ttctacatcc tcacgggcag
     1261 cgatacctgg ctcgagccgt acgccacgat cctgatcctg gcggtcgtcc tcttcctgca
     1321 gggcctgctc aacacgttca gcgtgcgcct cgtcgccctg ctcaacgacg tgtcggtgta
     1381 ctggcacctc atcggcgtgg cgatcatctt cgtgctgctc ttctgggcgc cggagtcggg
     1441 cacccaccag tcgatcaact tcctgttcgg cgctgacggc tggaacgcgt tcgaggggct
     1501 ctcggggttc accttcgggt tgtacgtgtt cctcatcggc ctcctgaacg cgcagtacac
     1561 gttcaccggc tacgacgcct cggcgcacgt ctctgaggag accatcaacg cgcgcatctc
     1621 ggcgcccaag ggcatcgtca actccatctg gatctccatg gtcgcaggct tcatcctgct
     1681 gctcggcgtc agcatggcga tcccgacggc gttcccggtg accatcaacg gcgtcgagta
     1741 cgccggctac gacgccatcg ccaccgacct cgtgccgtgg gcgaccatct tcgagtacgc
     1801 caccggccat accatcggcc tgctgctcgt cctgatcgtg atcgtggcgc agttcttctg
     1861 cggcatgtcg tcgatcaccg ccaactcgcg catggtgtac gccttcgccc gtgacggcgc
     1921 ggtgccgttc tcggacttct ggcaccacgt cagcaagcgc cagcgcgtgc cggtgcgcac
     1981 cgcctggttc ggggccatcg gggcgttcat cctcgcgctg ccctacctgt acaacgtcgt
     2041 ggcctacgcc gcggtcacgt cgatcgccgt catcggcctc tacatctcgt acctcacgcc
     2101 ggtgttcctg cgccgcatca acccgggcgt gttcgagccg ggcccgtgga agctcagcaa
     2161 gacctgggga ccgatcatca actggatcgc catcatctgg gtcgtcttca tcgtcatcct
     2221 gctgatgctc ccgcagtact cgccgggcaa caccgtcgag acgttcaact acgcgccggt
     2281 cgccgtgctc gtcgtcgtcg gcggcgccgg actctggttc gccgtctcgg cccgcaaatg
     2341 gttcaagggc ccgaaggtcc agggtaccgc cgaagagctc gccgagatcg agcgcgacct
     2401 caacgtcatc tgagtcgcgc gccatcgcgg atctgctccg gaacggggcg ggcctcacgg
     2461 cccgccccgt tcccttgcgg ggacgctcct tcctgcggca tgaccggcgt ggcccggccg
     2521 ggatcggtgg cagggccgct acgggccgct ttgacctgtc gcgcccgcgc gagcaggctc
     2581 gcagtgaggc cggtccgccg ccctcatgga gcggaccgag ctgtcttccg atggcccgcc
     2641 cggccaacgt acggccgcct gctagacaca cgggacggag tacactacga tgcggactga
     2701 tggagcgaat atgtgcagtt gagtgactcc ttgttcactt ttcaccaccc cacggaatcg
     2761 atcctcgagg aggagcaatg aaagacccca atctctttga agacctggta gcggtcagtc
     2821 ccggcatgga gatctggtgg gactcgtcgc cggtcatctt cgccaactgg tgcgacaaga
     2881 tgctggccaa ggccgacgag gccgacaagg ccgcgctcaa ggcccagttc gcgcgcatgt
     2941 acaacgccga cgacccgatg agccagctct tccggggtgt caccaccaac ccgcccctct
     3001 ccctcgccgc cttcaaggac gacccggcgc gctgggagaa ggtcgcccag aaggtcctcg
     3061 ccgagaacaa ggacgtcgac accgagggcc tcttctggct gctctacaag gaagtcgtcc
     3121 ggcagggctc cgacatgttc ctccccctct tcgaggcgtc gggctacaag gagggcttcc
     3181 tctccggcca ggtcgatccg cgcagcgcct tcgacggcga ggccatgctc gcgcaggcgc
     3241 tcgagatcca cgccgtgaac cccaacgtga tggtcaaggt ccccggcacc gcccagggct
     3301 acgaggtcat cgaggagctc acctcgcgcg gcatcgccac caacaacacg ctcaccttcg
     3361 tgctctcgca gttgctcgac tgcgccaagt cggtcatgcg cggcctcgag aaggccaagg
     3421 ccaacggtgt ggacctgagc aagtggcgct cggtcatcac gcacatggag gcccgctacg
     3481 gcgacctcgg cggcctgcgt gaattcggcg ccgaggctgg cgtcgaggtc accgaggccg
     3541 acgtccggta cgccgagctc gcgatcttca agaaggccta ccgcaccgtc aaggagcgtg
     3601 gctaccagag caagatgctc tcctgctccc tgcgtctcgg cccgaccgtg gacggcgagc
     3661 agcgcatctg gcacctcgag gagaaggccg gcgccagcat cgtcgtcacc gtgccgccgc
     3721 cgttcatcga cgaggtcatc aacttccccg gcgccgacag catccacttc caggccgacc
     3781 agatcgacgt cgacccgccg gccgacgtga tggagaagct gctcaagatc ccgtacttcg
     3841 cccgcgccta cgacgaggac ggttacacgc gcgaggagta caacacgcat ccggccctcg
     3901 tgaagacaca cgaggccttc tccaaggcca ccaacgagat ggtcgcgttc gccgacggtt
     3961 gcctcaaggg cgactgcaag gcctgacagc ggggactcac ggtcacctga ctgacgaaag
     4021 gacagtgggc atgacggaca tcaaagacaa gatcaacccc tacatctgga cggctcccga
     4081 gtacaacacg ggcgtcctcg acatggcgtg ggcgcacccc gagttgggcc gcatgatgct
     4141 caacgagaac ccggtgcccc cgtcggacgc ggtggtcgcc gccatcaccg acatggcgaa
     4201 gaagggcaac cgttacccgg accgcgccta tcgcctgcgc gagaagctcg gcaagctgca
     4261 cggcgtcgcg gcggagaaca tcgcccttgc caacggctcg tccgagacca tcgacgcgat
     4321 gatgcgcatc ttcctgcagc cgggcgacga gttcctctgc tccaacccga ccttcgagat
     4381 gttcccctcg cgcgccggct actgcaacgc caagacggtg caggtcccgc tccgcgacga
     4441 ggacctgcag tacgacgtcg agggcatgct ggccgccgtc aacgacaaga ccaagctgat
     4501 cctcatcatc aacccgaaca acccgacggg catcttcatc gaagacgccg acctcaccag
     4561 gttcgccgag ctcggcatcc cgatgtgcat cgacgaggcc tacctcgact accacccgca
     4621 ggtcgaggcc aaggtcgact tcgtcaagaa gtacccgcac gtgttcctct cgcacacctt
     4681 ctccaaggcg ttcgggctcg ccggcatccg catgggctac atggtcggca gccccaccgt
     4741 ggtggacgcg ttcaacctca tgtacctgcc ctggaacctc agcctcatgg cgatcgccgc
     4801 ggccgaggcg atcctggaca cgggcgaggt caagcagaag gtcgagtaca acaacgactg
     4861 gatgaagaag ttcgccgacg cgttgtcggc caagggcctc aagccgttcg aggcgcacgg
     4921 caactacatg ctcgtcgacg cttccgcgag cggcaagacc agcgccgaga tcgtgcaggc
     4981 ggccaacgac acgcagcagg tcatcatcaa gaccatcaag cccatcaacg gcaaggaagg
     5041 ctacttccgc gtcactcccg gcactcccga ggagtccgag cgcttcatgg cgttcatcga
     5101 ggcgtacttc tgagccgact gacgctggcc tgacgttcgt ccgcagcctc cgccctcccc
     5161 cggtcacctt gggagggcgg aggctgacgg ccctcaagaa aggatccgaa tgagcacagt
     5221 ggtgagcgat cccgcgctcc aggacaggtg catcgacacc atccgcttcc tggccgtcga
     5281 cggcgtccag aaggccaact ccggtcaccc cggcatgccc atgggcgctg ccgccgtcgc
     5341 ctacacgctg ttcacgcgtc acctgcgcta cgacccggcc gacccgcagt ggccgaaccg
     5401 cgaccgcttc gtgctctcgg ccgggcacgc gagcatgctg ctctactccc tgctccacct
     5461 gaccggctac gacctcaccc tggacgacct caaggcgttc cggcagtggg gcagccgcac
     5521 gcccggacac cccgagtacg ggcacaccgc cggcgtcgag accacgaccg gcccgctcgg
     5581 ccagggcgtc gccaacgccg tgggcatggc cgttgcggag cggttcctcg ccgccaccta
     5641 cggcgacgtc atggaccact acacctacgt gctcgccggc gacggctgca tgatggaggg
     5701 catcagcagc gaggctgcct cgttcgccgg ccaccagcaa ctcggcaagc tcatcctgct
     5761 ctacgacgac aaccacatca ccatcgacgg ctccaccgac ctcgccttca ccgaggacgt
     5821 gtgcaagcgc tacgaggcct acggctggca cgtgcagaag gtggccgacg gcaacgacgt
     5881 ggaggccatc gacgccgcga tcacggcagc caaggcggag accggcaagc cgtcgttcat
     5941 cgcggtgcgc acgcacatcg gctgcggctc gcccaacaag caggacaagg ccgccgccca
     6001 cggcgcgccg ctcggcgccg acgaggtcaa gctgaccaag gagacctgcg cctggccgct
     6061 cgaccccgag ttccacgtgc ccgacgaggt ccgcgaggcc ttccgcgccg ccggcaagca
     6121 ggccgccgag gcccacgccg agtggacgcg caagtacgag gcgtggcgcg cggccgacgc
     6181 cgcccgcgcc gcctcgtggg acgacgcctg ggcgcacgcc ctgcccgagg gctgggacgc
     6241 ggacctgccc gtcttcacga ccgaggacaa ggtcgcgacc cgcgccgcct cgggcaaggt
     6301 catcaacgcc gtggcgccgc acatcccgac gctcatgggc ggctctgccg acctggcccc
     6361 gtccaacaac acgctgatca aggacgcacc cgaccagcag gcggccacgc ccggcgggcg
     6421 caacgtgcgc tggggcgtcc gcgagctcgc catggccgcc atgggcaacg gtctcgcgac
     6481 gcacggcggc atccgcccgt acgtggccac gttcttcgtc ttcgtcgact acatgcgccc
     6541 ggccatgcgc ctctcggcgc tgatgggcct gccggtcatc tacgtgctca cccacgacag
     6601 catcggcgtc ggtgaggacg gtcccaccca ccagcccgtc gagcacctcg ccatgctgcg
     6661 cgccacgccg ggcttcatcg acctgcggcc ggccgacgcc aacgagacgg tcgaggcctg
     6721 gaggatcgcc ctgcagaccg ccgacgctcc cgtagggctc atgctgacgc gccagaacct
     6781 gccgacgatc gaccgtgaga agtacgcgcc ggccagcggc atcgcccgcg gcgcctacgt
     6841 gatggacgac gcctcggacg gcggcgcgcc ggatgtgatc ctcatcgcca gcggcagtga
     6901 ggtcgcgctc gccctggagg cgcacgagcg cctggtcgcc gagggcgtgc gctcgcgcct
     6961 ggtcaacatg gcctccatgc ggctcttcga gcagcaggac gccggctatc gcgagagcgt
     7021 gctgccgccg gcctgcggcg cgcgcgtcgc ggtggaagcc ggcgtcacgt tgggctggga
     7081 ccgctggatc ggcgacggcg gcgtggtcat cggcatcgac cacttcggcg cctcggctcc
     7141 tgccggcacg ctgttccagg agttcggctt caccgccgac aacgtctacg cgcgcgccaa
     7201 ggccctgctg gcctgacgcg gcgcgagcgg cgcggcggcc tctcgccgcg ccgacggtac
     7261 gacgagacgg ccgccccgcg cccgcggggc ggccgtctcc gcgtctccgg agcggcggcc
     7321 cctgccgccc caagccaggc ccggacggac cccggcatcc gcgccgcgtg cttgaaccgc
     7381 cgcgaggccg accctatgat gtgttcgact atgtgcgaac ttgtagcacg atgagcagcg
     7441 accgcgaggg gcagcaaatg tcgccagacg taccttccga agtccacgag atgatccccg
     7501 cggagcgccg cgcgcgcatc gtcgagctcc tcgaggagcg gcgcgcggtc agggtctccg
     7561 tcctcagcga gacgctcggg gtgtcggaga tgaccatccg tcgcgacctc gaactgctcg
     7621 agcaggcggg cctgctctcg cgcatgcacg gcggcgcgat cctcaagcga cggatgatcg
     7681 aggagccgct gtacgccacc aacgtgcgcg cccactcgga gcagaagcgg cgcatcgcca
     7741 aggcggcggc ggggatgatc cagccgggcg agaccgtctt cctcagctcc ggcaccaccg
     7801 ccgcccaggt gctcagccac gtcgacccgc agctcaaggc gcgcatcgtc acccacaatg
     7861 tcggggcgct ctccaccgcc cagagcagct ccctcgacat cgtgctcctg gggggctcct
     7921 accggcagcg ctccaacacg ctcgagggtc cgctggcgat cgaggccgtc tccacgttcc
     7981 acgccggccg cttcatcctg ggggcggacg gcgtgagcct cgaggagggg gtcaccaccc
     8041 ccagcatcgg cctggccggc gtcgagcggg ccatggtccg ccagacgcgc ggcgagatcg
     8101 tcgtgctcgc cgacgccagc aaagtcggcg tcatcggcga cgtggccatc tgcacgctgg
     8161 acaaggtcga cacggtgatc gtggacgacg cggtggacaa ggacgtgcgc gacgagatgg
     8221 agagactggg gatcgtccag atcgtcgtct gacgggcggc gaggcggcgc ccgacgcgac
     8281 acggagcggc gccggacgcc cggccctcag aggtcccagt acgccgactg cacctcgcgc
     8341 gcccgtgcca ccgcggcggc ggcgcgctcc gtgccgccgc tcacctccgg tgcgcgctcg
     8401 tcggcgaccg cgcgcagcac ggcctcgacc gcccgcgcgc tggcaacgcg gtcgtagccg
     8461 ccctccagca cgaacgcgag cctgccctcg cacagttcct gcgccagacg cacgcagcgc
     8521 agcgccatct gcgcgaagcc ggcctcggtg acgcgcatct cgcccagcgg gtccgagcga
     8581 tggatgtcct ggccggcgga gacgaagatc gcctgcggcg cgaactggcg gacgatgggc
     8641 tcgaacagcg cgtcgaagac gccggcgtag tcggcgtcgt cggcgccggc gggcagcggc
     8701 acgttgacgg tggacccctc acccgcaccc tcgccacact cgccgaagaa cccgctgccg
     8761 gggtagtgcg gccactggtg cgccgagccg aagaggatcc tgggctcggc gtagaacgcg
     8821 gcttgggtgc cgttgccgtg gtgcacgtcc cagtcgagga tggcgatccg ctcgtatccc
     8881 cgctggagca gcctggcggc ggcgatggcc acgttgttga agaggcagaa gcccatcgcc
     8941 ctgtcggggg tcgcgtggtg cccgggcggg cggatgagcg cgaacggcgc cagcccgtcg
     9001 ccccagagct cggccgcgag cagcgcgccc ccggcggaga gcagcgccgt ctcgtagctg
     9061 cgcggcgaca cgtgcgtgtc cggatcgatc caggtgccgc gtccctgcgc cgcgcgcttg
     9121 atcatcgcca ggtgcgccgg cgtgtgcacc aggaggacat cgtcctcggt ggccggcggc
     9181 ggcgaggtga gcacggtgag acgcggccac agatcggtgc cctcgaggta gtcgatcgtc
     9241 gagctgacgc ggtccgcacc ctcggggtgc tcgccggtgt cgtgctcggc gtaggccgga
     9301 tcccagatga cggcggcgtc catggcgcca aggatacccg accgccgggc gccgggacca
     9361 tccgcccgtc cgccgccgcg acgaaggcct acgcggcgct cccgcctccc acggtgaggt
     9421 agggccgcgc aaggcgctcc cagagctcgg tggccgcgcg cacgtcggcg ccctcggggt
     9481 ccaggccgtg ctggatggcg agcccgtcca cgatcgcgat catgagcatg gcgaacggcc
     9541 gcaggtcggc cggcgcgtcg ggaccggcgg cgtccaggca gcgcagcacc gtctcacggt
     9601 agccgtcgta gagggccgcg acccgctcgc gcaggtccgg gtcgcgcagc gcgtgcggca
     9661 ggacgtcgaa gaacgactgg aagctctcgg cgtcggcggc gatgcgcgac tcgccgtcga
     9721 tcagcgcgtg cacgcgggcc tcccccgccg gcgtggccgc cgtctcctcg atgaggccgc
     9781 ggttggagtc gtgcgcgagc gagtcgacca gggcggtgac gaggccggcc ttgttgccga
     9841 agtgatagcc gatgctcgcc ttcgactcgc cggcctcgcg cgcgatggcg gagagcttga
     9901 gcgcctcgaa cccgccttcg gcgagcagcc gctgggccgc cgccaggatc cgctgcgcgg
     9961 tcggcggcag cgcctccatg ggatcctcga gcagcagcgg ctcgggcagc ccctccccgc
    10021 gcgccaggct ccttcttctc tcatcctgtg gcgacatcag gtcaccatcc gttcgccgat
    10081 cgtagttgcc aggctctgca taccagtgta atctacattt gtccgtccgg acaaacaaac
    10141 tttcggcgtc cacgtcgtgc gcgactcgtc gaccaggagg aaccgagttg gataccacgc
    10201 tccccgccct gaagaagtac cgcggccgtc acttcctgac cgacctcgac tacacgcgcg
    10261 aagagctcga ggacctgctc aagctcgccg tgggcctcaa ggccctgtgg cggaagcgcc
    10321 cactcacccc gttcctcccc ggcaggcacc tggcgatgat cttcgaggcc gcctcgacgc
    10381 gcacgcgcgt cagcttcgag aacgggttcg ccgagctcgg cggcaacgcc ctctacctgc
    10441 gcccgggcga gatccacctg cccggccgcg agagcatcgc cgacacggcg cgcacgctct
    10501 cgcagtacaa cgccgccatc gagatccgca gcaagaagaa cgagaccgtg aacgagctcg
    10561 ccgcgtggtc ggcggtgccg gtgatcaacg gcatggacca cgaccggcac ccctgccaga
    10621 gcatgagcga cgcgctcacc atcctggagc acgcgggcac cctggccggc gtcacgttcg
    10681 cctacgtcgg cgacgccgcc cacccgtgca actcgctctc cacgacgctg accaagctcg
    10741 ggctgaacgt ccgtgtcggc aacgccgccg gctacgagat ggcgcccgag ctcgtcgagc
    10801 tctccacgcg gttcgccgcc gaggccggcg ggtcgttcct gctcaccaac gacccgctcg
    10861 aggccgtcgc cggcgccgac ttcgtctaca ccgactgctg gtggtggacc ggccaggagg
    10921 acgagtacaa ggaccgcgtc gaggcgttca ggcccttcca ggtcaatccc gacctgtgga
    10981 gccacgccaa gcccgagacg aagttcatgc actgcctgcc ggccatgcgc ggcgaggagg
    11041 tcgtcgacga gatcgccgac ggcccgatga gcatcatctt cccgcaggcc cagaaccgga
    11101 tgcacttcca gaaggccctc atgctcgccc tgatcggcat cgacgagctg cccgccgacc
    11161 cggacctgca gccgatcggc aaggccctgc tcagctaagc gacacacgca gcacccgcgt
    11221 cagcgaaagg aaccacgatg gcaaccgacc tgcacgatgg agcgccgggg ggaacgctca
    11281 ccgagaagcc gaaggtgttc ggcctgttca gcatggtcct gttcagcgtg tcggcgatcc
    11341 tcgtcgccga taccgtgggc agttccgccg cgatgggcgt ccagggcctc gggttctgga
    11401 tcatcctcgg cctgctcttc ttcatcccgt acggcttcgt caccgccgag ctgggcagcg
    11461 cctggcctga cgagggcggc atctacgtgt gggtcaagac cgccttcggc cccggctggg
    11521 gcacggtgac ctcgtggatg tactgggtca acgtcgccct ctgggcgccc tcggtgttcg
    11581 tcctcttcat cggcgccatc gaggtcgcct ggggcgtgga cgtgccgccg ctctgggagg
    11641 ccgtgatcgt catcgccatg gtgtgggcga tcgtgatcgt cggcatcctg ccgctggcgt
    11701 ggagcaagtt cgtgcccgac ctgagcgcgg cgctcaaggt catcgtgctc gtcggcctgg
    11761 gggcgctggg cgtcgcctac gtggtccaga acggcgccgc caactccttc gcgctcagcg
    11821 cctggatccc ggagtggaac accgacagcc tctccttcct cccgatcatc atctactcgt
    11881 acatggggtt cgagctcatg agctcggccg gcgggaacat caaggacccg aagcgcgacg
    11941 tgcccaagat gatcatcctc gccggcgccg cgatcctcgc cgtatacatg ttcgcgacgt
    12001 tcggcatcct cgcgtcgatc aagacggagg acgtcagcat cgtcaccggc atcaccgacg
    12061 ccctcaagct catgttcgac gaggtgctcg gaggggcgtc ctggctgttc aacatcgcca
    12121 tcgtcgccct cctcttcacg ttcttcggca acatggtgac gtggtcgatc ggcgccaacc
    12181 agaccatcgg cgccacgggc ctggacgaga cggcgccggg cgtgttcggc cacgtcaaca
    12241 ggcgcttcgg cacgcccgac tacgccttcg tgctcatggg cgtcatcgcc accgcgatca
    12301 cgatcctggc ctacgtgctc gacccgaacc agacaagcgt gttctggacc ctgttcgcgc
    12361 tctcgtcgat cgtcttcctg atcccctacc tgctgatgtt cccggcgctg ctcgtgctgc
    12421 gccacacgca cgcggaccag ccgcggccgt acgtgatccc gggcggcaag gccggcgcct
    12481 ggatcgccac gatcctctgc gaggcgggga tcgcgctcac gctcttcctc ttcttctact
    12541 tcccggcgga gggcgtgtcg aagggcgtgt tctggctgat caccggtggt ggcacgatcg
    12601 tctcgctcct cataggctgg tggctctact ggcacgccag ccacaagccc ggcggccgca
    12661 acgaggccga gaccgtcgac gcataaggac cagaggaccc agatggcagc aacaggcaag
    12721 cagcgcggca agaagaccgt cgtcgtcgcc ctcggcggca acgccatcct ccagcccggc
    12781 caggtcggca ccttcgagga gcagctgttc aacatcgacg gcgccatgcg ccgcgtcgcg
    12841 gccctcgccg ccgacggctg gcgcgtcgtg ctcacc
//
LOCUS       DASUMX010000272         3850 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1394204, whole genome shotgun sequence.
ACCESSION   DASUMX010000272 DASUMX010000000
VERSION     DASUMX010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3850
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1394204"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VFZ86_12990"
     CDS             <1..222
                     /locus_tag="VFZ86_12990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HEX5643234.1"
                     /translation="APRPAPPSPEPPRPVPDDGAARARLGDAEAALSRAETEERAARR
                     HWKDAQSELESARAAVEAAQRDLDRLQGR"
     gene            complement(258..587)
                     /locus_tag="VFZ86_12995"
     CDS             complement(258..587)
                     /locus_tag="VFZ86_12995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643235.1"
                     /translation="MTAEVAPDAAGRNGLVCRYRFGGLFARLIRRDGYDQLKGNLQYI
                     AAAICHRAEELHAASSDGRPPPREAGRSPDHYDTRDRGAVARPTPVVRTVTTVGAATH
                     QTPMAAR"
     gene            complement(896..1561)
                     /locus_tag="VFZ86_13000"
     CDS             complement(896..1561)
                     /locus_tag="VFZ86_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016144032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEX5643236.1"
                     /translation="MDAILRTGQFFLHVDTHLATLVEHFGQWVYGILFAVVFCETGLV
                     VTPFLPGDSLLFAAGTLAGAGVLDPLVLGVTLLAAAILGDAVNYFVGRFFGARLLSRP
                     RRFVRPDHIARTEAYYARHGGKTIILARFVPFARTFAPFVAGVIRMDPLRFLLFNVTG
                     AALWVGLFVGGGVWFGNIPWVKDNLALVLVAVVLVSVGPAVVGMARRRLQGVAGGSQE
                     DAS"
     gene            1728..2717
                     /locus_tag="VFZ86_13005"
     CDS             1728..2717
                     /locus_tag="VFZ86_13005"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEX5643237.1"
                     /translation="MKAIVCTAYGDPDVLKLEEVEKPRPKQGEVLVRVHATTASRADC
                     EIRRFEFPAWIWLPIRLAFGVVRPRVRILGQEFAGEVAALGDGTTSYAPGDRVYGTTG
                     IGLGAYAEYVCAGGRRAGSIVSPMPATLSYAEAAAVPYGAMEAHRFLGKAGVGAGQRV
                     LIIGAGGSFGTYAVQLANHAGAEVVAVDSAGKLEMLREIGAAQVIDYTAQDWDDGAGD
                     FDIVFDVIGRAPFGRVVRLLRPGGRYVVGMPHTAQVIRGLWASLTGGKKMVFSSGTAT
                     GEHLRAVTELIEAGALRPVIDRQYPLEQMAEAHRYAETEQKLGNIVIVVGTET"
     gene            complement(2750..3526)
                     /locus_tag="VFZ86_13010"
     CDS             complement(2750..3526)
                     /locus_tag="VFZ86_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010836881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5643238.1"
                     /translation="MTRTLSDTWTLARRSLRHITRSPDTVITVLLMPIALMLLFVYVW
                     GGAMGQQTGSVRYIDFIVPGIIVMTVLSGISYAAVRLNMDVQSGIINRFRTMPVAPSS
                     ILGGQVAASTVSNLFSSLVVLLVAFAMGFRSEAGVTGWLVFAGILLLFTLATTWLAIF
                     FGLLAKTMEGAGAFGYILLLLVFISPSFVPTTSMTPLLRGFAEHQPMTPVVETMRSLL
                     VAGTAGPQVWTALAWILGLLAVSYLLSVAVYKRKAIVVNT"
     gene            complement(3523..>3850)
                     /locus_tag="VFZ86_13015"
     CDS             complement(3523..>3850)
                     /locus_tag="VFZ86_13015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEX5643239.1"
                     /translation="IAILHQGRIVASGTAGELKKLAPGGRIELTFHDEETRAAAAREL
                     GESYACVEGAGATLTIATSGTVAEVAGIFAGLRDARIEPTEFSQKVATLDDAFLKITG
                     SGREDS"
BASE COUNT          655 a         1327 c         1344 g          524 t
ORIGIN      
        1 gcgccgcgtc cggcaccgcc ctcgcccgag ccaccgaggc cggtgccgga cgacggcgcg
       61 gctcgcgcca ggctcgggga cgcagaggcg gcgctcagca gggccgagac cgaggagcga
      121 gcggctcgac gccactggaa ggatgcccag agcgagctcg agagcgcacg tgcggcggtc
      181 gaggccgccc agcgcgacct cgaccgcctc caaggccgct gaaagcagag accggcgata
      241 tcacatcgcg gcgatgttca gcgcgcagcc atcggggtct gatgcgtggc cgcgccgacg
      301 gtcgtgaccg tacgcacgac aggggtgggc cgtgcgaccg cacccctgtc cctggtgtca
      361 taatgatccg gcgacctgcc ggcctcgcgc ggaggaggcc ggccgtcact gctggccgca
      421 tggagctcct cggcgcgatg gcagatcgcc gcggcgatgt actgcaggtt ccccttcagc
      481 tggtcgtagc cgtccctcct gatgaggcgt gcgaacaggc cgccgaagcg gtagcggcac
      541 accagaccat tccgcccggc agcgtcgggt gcgacctcgg cggtcaacct gccctccagg
      601 gcccgtaccc gggtccttcc tcgccaggtc agtgtgagcg acggctccgg gagcgatccc
      661 agatcctcca ttgcgcgtct cagctcaccc gctggcgcgg acgtctcctg tctcaccgtg
      721 agctcgttca tgcctgcctc cttcgtgagc agtcggaccg cgatgagtcc cctacttctc
      781 atctgctcgt tcctggcggg gtctaactcg ccggacaggc catggttccg caggccgttg
      841 gccaagaggt ggaacgcagg tccggagcca cgaagagcgt agccgactgc tggggtcagg
      901 aagcatcctc ctgcgacccg ccggccacgc cctgcaggcg gcggcgcgcc atgcccacga
      961 cggccgggcc gaccgagacg agcacgacgg ccacgaggac cagagcgagg ttgtccttca
     1021 cccaggggat gttgccgaac cacacgccgc cgcccacgaa gaggccgacc cagagggccg
     1081 cgccggtcac gttgaagagc aggaaccgca gcggatccat gcggatgaca ccggcgacga
     1141 agggcgcgaa ggtccgcgcg aagggcacga agcgcgccag gatgatggtc ttgccgccgt
     1201 ggcgcgcgta gtaggcctcg gtgcgggcga tgtggtcggg ccgcacgaag cgccgcggcc
     1261 tgctgagcag cctggcgccg aagaagcggc cgacgaagta gttgaccgcg tcgcccagga
     1321 tggccgccgc cagcagggtc acacctagca ccagcgggtc gagcaccccg gcgccggcga
     1381 gcgtgccggc ggcgaagagc aacgaatcgc cgggcaggaa cggcgtgacg accaggccgg
     1441 tctcgcagaa cacgaccgca aacaggatcc cgtacaccca ctgtccgaag tgctccacga
     1501 gcgtcgccag gtgcgtgtcg acgtggagga agaactggcc ggtgcggagg atggcatcca
     1561 tggcgcggtc ccttcgtctg gccgcgacta cgcggccggg cggtggcgga gcgggtaccg
     1621 taccggctcg ccctggcggc ggcaacccga ctccgggcgt caggacggag agtgcgagat
     1681 aatggcctcg acaggcgcgc gcgactgcga gggactgcga ggtcttcatg aaggcgatcg
     1741 tctgcactgc ctacggcgac ccggacgtcc tcaagctgga ggaggtcgag aagccgcgcc
     1801 cgaagcaggg tgaggtgctc gtccgcgtcc acgccacgac cgcctccagg gcggactgtg
     1861 agatccgccg gttcgagttc cccgcgtgga tctggctgcc catccggctg gcgttcggcg
     1921 tcgtgaggcc gcgggtgcgc atcctcggcc aggagttcgc cggcgaggta gcagcccttg
     1981 gcgacgggac gacctcctac gcgcccggag accgcgtcta cggcacgacc gggatcgggc
     2041 tcggggccta cgccgagtac gtctgcgccg gcggacggcg cgccggcagc atcgtgtccc
     2101 ccatgccggc gacgctgagc tacgccgagg ccgccgccgt cccctacggc gccatggagg
     2161 cgcaccgctt cctgggcaag gcgggcgtcg gcgccgggca gagggtcctc atcatcggcg
     2221 ccgggggcag cttcgggacc tacgcggtcc agttggccaa ccatgccggc gccgaggtgg
     2281 tcgccgtcga cagcgccggc aagctcgaga tgctccgcga gatcggcgcc gcccaggtga
     2341 tcgactacac cgcacaggac tgggacgacg gcgcggggga cttcgacatc gtcttcgacg
     2401 tcatcggccg ggcgcccttc ggccgcgtcg tccgcctgct gcgtccgggc gggcggtacg
     2461 tggtgggcat gccgcacacg gcccaggtca tccgcgggct ctgggcctca ctgaccggcg
     2521 gcaagaagat ggtcttcagc tcgggcaccg caacgggcga gcacctccgc gccgtcacgg
     2581 agctcatcga ggccggcgcg ctccgacccg tgatcgaccg ccagtacccc ctcgagcaga
     2641 tggccgaggc tcaccgctac gccgagacgg agcagaagct gggcaacatc gtcatcgtcg
     2701 tcggcacgga gacctgacca gccgcccggt ttcactggga cgcgcccgcc tacgtgttga
     2761 ccacgatcgc cttgcgtttg tagaccgcca cggaaagaag gtacgagacg gcgagaaggc
     2821 cgaggatcca cgccagcgcc gtccagacct gcgggccggc ggtaccggcg accagcagcg
     2881 aacgcatcgt ctcgaccacg ggcgtcatcg gctgatgctc ggcgaagccc cgcagcaacg
     2941 gcgtcatgga ggtcgtcggc acgaacgagg ggctgatgaa cacgagcaga agcaggatgt
     3001 agccgaatgc gcccgccccc tccatggtct ttgcgagcag gccgaagaag atggccagcc
     3061 atgtcgtcgc gagcgtgaac aacagcagga tcccggcaaa gaccagccat cccgtcaccc
     3121 cggcctccga gcggaagccc atcgcgaacg ccaccagcag gaccaccagg gacgagaaga
     3181 gattggacac ggtggatgct gcgacctgac cgcccaggat ggacgacggc gcgaccggca
     3241 tcgtcctgaa gcgattgatg atgccgctct gcacgtccat gttgaggcgg acggcagcgt
     3301 aggagatgcc gctgagcacc gtcatgacga tgatgcccgg cacgatgaag tcgatgtagc
     3361 ggaccgagcc cgtctgctgc cccatcgccc caccccacac gtagacgaac aggagcatga
     3421 gcgcgatcgg catcagcagc acggtgatga ccgtgtcggg actgcgggtg atgtggcgca
     3481 gactgcgcct ggccagcgtc caggtatctg agagcgtgcg cgtcatgagt cctcccggcc
     3541 gctgccagtg atcttcagaa atgcatcatc gagcgtcgcc accttctggg agaactccgt
     3601 cggctcgatc cgggcatccc ggagtccggc gaagatgccg gccacctccg ctacggtgcc
     3661 actggtggcg atcgtcagcg ttgctcccgc gccctcgaca caggcgtagc tctcgccgag
     3721 ttcccttgcg gcggcggcgc gggtctcttc gtcatggaag gtcagctcga tccgcccacc
     3781 cggcgcgagc ttcttcagct cgccggcagt accactcgcg acgatacgac cctggtgcag
     3841 gatcgcgatg
//
LOCUS       DASUMX010000273        12586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_530867, whole genome shotgun sequence.
ACCESSION   DASUMX010000273 DASUMX010000000
VERSION     DASUMX010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 12586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12586
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530867"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="VFZ86_13020"
     CDS             <1..540
                     /locus_tag="VFZ86_13020"
                     /inference="COORDINATES: protein motif:HMM:NF013213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX5643240.1"
                     /translation="FEELAARPRVVAVGEVGLDFYRDLSPRDAQRAAFRAQIALARRV
                     GLPLVVHVREAGDEAMAQLAAEAGDLTVIMHCFSLPEYVDECNERGYYASFAGNVTYK
                     NAAELRAAAATVRDDRLLVETDAPFLAPVPNRGNSNVPAWVAHTAALVAEVRGVPAAE
                     LAELTTANARRVFGLAERD"
     gene            542..1435
                     /gene="rsmA"
                     /locus_tag="VFZ86_13025"
     CDS             542..1435
                     /gene="rsmA"
                     /locus_tag="VFZ86_13025"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEX5643241.1"
                     /translation="MGASGGRPGAGDRSRPPRPHALRRYGQNHLVDKNVLHAIVDQAA
                     VSPGDVVLEVGAAGGLLTRPVLERARFVHAFEIDRRWLPRLEELAAAEPRFAVHAGDA
                     LKADLGALEPPPTALVANLAYNIAIPLIMTTIAELPSVRRWAVMVQRELGERLFAVPS
                     TKAYAAVSVLTQLACRLEKARPVPASAFRPRPRVESSFVTFVRRETEDDADGGTPDAV
                     AAAPGPAEYAAVSRLVRLAFGQRRKTLVNSLGGAAHAHVTLSRDDVRAALRDLGLSEA
                     ARPEELAPPQWLTFARTVGRA"
     gene            1494..2429
                     /gene="ispE"
                     /locus_tag="VFZ86_13030"
     CDS             1494..2429
                     /gene="ispE"
                     /locus_tag="VFZ86_13030"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEX5643242.1"
                     /translation="MTSDGVALEGDRAEVVAPAKLNLALLVGPVRPDGFHEIASLMLP
                     VSLADRVTVERTPGAGLDVVCDVAPGADNLAAKVVRELEQRLDRSFDVRVTIDKRVPH
                     GGGLGGGSSDAAATLIALERLYDLDLSSRVRYEAAAAVGSDVPFFLWPGAQLAMGRGQ
                     VLKAVQLPALHLVVAMPDLGLSTAAVYRWRDEDAEVTLKEFASRARLLSSRIQAAATP
                     GDVAALVANDLEASVVARKEKVRELLERLRSAGALAAAMTGSGAACFALFDGELEALR
                     AREAAAPARAWYVTDLQPEGRTKPAPRRGRGKPPA"
     gene            2494..2568
                     /locus_tag="VFZ86_13035"
     tRNA            2494..2568
                     /locus_tag="VFZ86_13035"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2526..2528,aa:Gln,seq:ttg)
     gene            2630..4048
                     /gene="glmU"
                     /locus_tag="VFZ86_13040"
     CDS             2630..4048
                     /gene="glmU"
                     /locus_tag="VFZ86_13040"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEX5643243.1"
                     /translation="MTTADTKRPPLAVVILAAGLGTRMKSEVPKVLHEVCGRPMLSYV
                     IDAALSVSPERVVVVTGPEQEATAEILPVGCERAVQQERLGTGDAVRAGLEPLHDFEG
                     DVMVLYGDVPLVDGDFVAGLHQRHLEARAAATLTTVELDDPAHYGRVVRDETGGIQRV
                     VEHKDASGEERQIDEINVGLYVFRSDALRPALDRLGSDNAQGELYLTDAVHLLLDAEL
                     TVATFESDDEEMCMGVNSRVELAVVNAAMRRRLLERLMLAGVTVEDPETTYVDYGVEV
                     GRDTLIRANTHLLGRTTVGAASEVGPGSFLRDAFVGDRARVVASHLYECVISSGCSVG
                     PFAYIRPNTVLAEGAKAGTFVEIKNSRIGERSKVPHLSYVGDAIVGVDSNIAAGNITA
                     NYDGYDKHPTYVGDRVRTGSDTTIVAPVTIGDDAFTAAGSVITRDVPPGALGVAREQQ
                     KNIEGYAGRRAARRLREQETSG"
     gene            4149..5123
                     /locus_tag="VFZ86_13045"
     CDS             4149..5123
                     /locus_tag="VFZ86_13045"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017983559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEX5643244.1"
                     /translation="MTSPRKRLMIFSGRSNPVLAAKIADKLDVAPGSVELKTFSNGEI
                     YARFEENVRGSDMFIVQSPCDDINGELMELLIMIQAAQLASAKRITAVMPYYPYSRQD
                     KKSAAREPITARLVATLLEASKVDRVLSMDLHQGQIQGFFEIPVDHMTAVPMLADYFR
                     YKDFGDTPLVAVSADAGGVKLAKRFINRVPGCDLAVLTKMRPEHNVAETLHFIGDVHD
                     KIAIIVDDIIDTAGSVTSAIDTLYDRGAKEVYVTATHGIFSGPACERIRQSPVKEVVV
                     TDTVHIKLGLDDPKIKVLSVADILARTIRNVFEDESVSEIFAGDNQLF"
     gene            5134..5799
                     /locus_tag="VFZ86_13050"
     CDS             5134..5799
                     /locus_tag="VFZ86_13050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HEX5643245.1"
                     /translation="MDNVSIVVHKRDVTGSRAARRMRKSGLIPGVIYGHGKDAVLIAV
                     EPHALREALTTSAGTHAVLDVTFEGQKRGHKAIVKELTLDRVKSNVVHVDLQEIRLDE
                     TIETTVAIRFEGEAKGVKAGGMLDESTREVTVKGVVTAIPEHLVVDVSELDMNETLQV
                     ADLQVPEGIEVLADPEDVLCSVLPPRKVEAEEGAEGEEAAVAEAAAAEPELVGKKEDE
                     AEG"
     gene            5835..6434
                     /gene="pth"
                     /locus_tag="VFZ86_13055"
     CDS             5835..6434
                     /gene="pth"
                     /locus_tag="VFZ86_13055"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014374627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEX5643246.1"
                     /translation="MAVFRPSASVERLVVGLGNPGPAYEGTPHNIGYRVIEELARRHG
                     IPRARRGFDGRVAEGMVRGRTVLLLQPTTFMNDSGRSVAAALRAVKLRLEDVLVVHDH
                     IDLPFGRLRLMEGGGSGGHNGLKSITGLLGAGYARLRVGVDRPPSTDPDVVAEYVLSP
                     FRESRAEVDRVTAAAADAVELWLDAGLEAAANAVNRLDC"
     gene            complement(6375..7814)
                     /gene="dacB"
                     /locus_tag="VFZ86_13060"
     CDS             complement(6375..7814)
                     /gene="dacB"
                     /locus_tag="VFZ86_13060"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HEX5643247.1"
                     /translation="MGGTVIADALPARAGARGTRIRRTHAFLLLLALVLATACAGALC
                     PAPAVAGVDGAIAASLRQAGLHGAGTGVYVWDLDAALPVYELNAGTSLTPASNLKIVT
                     SAAAILDWGVDHRFVTELCAEDVPVAAGGVLQGDLYLRGLGDPSLSTRAYQQDVFGMT
                     TASFETFARTLKRQGVRKVRGRVLGDASWFDKLRTVPTWKDGLQLECGPLSALSGNQG
                     LRDGNRVSAPATYAAKLMKQALANVGVRVTGPSGTAKVPDTAKVVDRQYSAALRGILK
                     HMNKESDNAFAEMLLKGLGKEFHGDGSTAAGTRASKAALHALGVKTGTYVIQDGSGLS
                     YGNRMTAEGVVKVLGALRQRDDFDDFYDSLAIAGEDGTIHDRMRGTAAAGNARVKTGT
                     LNVAICLSGYVTSANDHLVAFSILMNGGSLDWERATRAQDAIVVALARSTLPGEPLLA
                     VTPVLRQHSVSAVETVHGVGGGLKPGVQP"
     gene            7928..9640
                     /locus_tag="VFZ86_13065"
     CDS             7928..9640
                     /locus_tag="VFZ86_13065"
                     /inference="COORDINATES: protein motif:HMM:NF012340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin like domain-containing protein"
                     /protein_id="HEX5643248.1"
                     /translation="MRRARTCLLLVVALASLAALSGGLPEGTAGAATAPAAFAGAAFA
                     ASSPGAPPWETAGPYPAAFDLRAEGRLTPVRSQDSYSTCWIMAAMGSLESVVLRKEGL
                     PLDFSENNLANHMSSRLDFEGMAPSELALAYYARWEGPVGESSDPYPRPGKSPPYLRA
                     VRHLQEALFLPERQGALDNDAVKWAVMTHGGVDAAVDFRVQDEFESWNASTFAYYNAS
                     RSTPNHHILVVGWDDAYPASKFLAGSRPPGDGAFLIKNSWGADFADHGYLWVSYHDST
                     IGDALVVFSGVEPAGNYDAVYQYDALGRSRWITAGGGEQAWFANRFTGAGAGRVAAVS
                     FYAPVPGTTYQVRVARALQGVAAAPVAAAGVLSVAGYHTVRLEEPAAVTAGEKFVVAV
                     RVTTPGWAEPVPVEAPSGLISPRARAGQSYVSADGAAWTDLTSHTGLSRANVCLKAFV
                     DAAGVADRRAPRVEIRGDVVRRGAQAVIPWRLTDPAFSSASAIVELSLRDAAGRLIAQ
                     RRIPAVAVGERGVWALRASWPRGRYSVRGRAYDVAGGRQATASRTAVVVGGVASAAPA
                     VPRR"
     gene            9762..>12586
                     /gene="mfd"
                     /locus_tag="VFZ86_13070"
     CDS             9762..>12586
                     /gene="mfd"
                     /locus_tag="VFZ86_13070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEX5643249.1"
                     /translation="MFLDYLLNDEKFEDAAPRLRRGAGAYAPSFYAPYVLAAALAGGG
                     GHVVVAPDGEAATRLAADLSVYLDVAVPVLPARGVLYGADVAPSPHVVGERQQARTTL
                     AAGGVVVAEAVALLERFLPLQLQPRPLTLSPGEEIDFDGAVARLAALGYQRIEQVRAR
                     GGFAVRGGLIDVYPALGDPLRVEFWGDEVERLRTFSIYSQRTTGEIRAATVFAAFEAD
                     TSQPEYVNGLHQAVAEWEREGREETPEELERRAAVRALAALAGRFTTLGEQAAAAGAG
                     WAVFNPDETYRALADFDGEVATAVPGAALRERLYAPLADARALLAKALRVDVVQRDQP
                     LQFAASRPQFAARDLASAERDLIRLVNDDYRVFVVFRHEGEAERATFRLRNLSAEVVT
                     PEQLARLGEAGPGLYFVAAPLRDGFVAEDLKLAVVNERTLIRSAPRERRFAAGTRLTT
                     FFDVRPGDHVVHEDHGIARFAGIETRTVAGVTRDYLLLEFRGDDRVFVPHDQIGKVSR
                     YVGASGATPSLDKLGGTHWQTVKTRARTAVVEMAGELLQLYAARQAIPGFAFPPDGEL
                     MRRLEEAFPYEETDDQAEAIDEVKNDLEAPHPMDRLICGDVGYGKTEVALRAAFKAAE
                     AGKQTLVLVPTTILAEQHWMTFEERFADLPVTVGMVSRFRSGAEQRKTLAAFSRGEVD
                     VLVGTHRLLSTDVRPKELGLVIVDEEQRFGVRQKELLRNLKLQVDVMSLSATPIPRTL
                     QMSLSGIRDISVIETAPRGRHEIRTYIGEYKDDLVRVAIEKELAREGQAFYLHNRVET
                     IDQAAEHVRALVPRARVLVAHGQMAERTLEKVMLAFLAGEADVLVTTSIIESGIDIPT
                     ANTLVVERADMLGLAQLYQIRGRIGRSDAHAFAYLLYPSDDLLTSEAAARLTTLSDHT
                     DLGAGFKIAMADLEIRGAGNLLGDEQSG"
BASE COUNT         1746 a         4601 c         4554 g         1685 t
ORIGIN      
        1 ttcgaggagc tcgcggcgcg gcccagggtg gtcgcggtcg gtgaggtcgg cctggacttc
       61 taccgcgacc tctctccgcg cgacgcgcag cgcgccgcgt tccgcgccca gatcgcgctc
      121 gcgcggcgcg tggggctgcc gctcgtcgtc cacgtgcgcg aggccggcga cgaggcgatg
      181 gcgcaactgg ccgccgaggc gggcgacctc acggtcatca tgcactgctt ctcgctgccc
      241 gagtacgtgg acgagtgcaa cgagcgcggc tactacgcga gcttcgccgg caacgtcacc
      301 tacaagaacg ccgccgagct gcgcgcggcg gccgcgacgg tgcgggacga ccggctgctc
      361 gtcgagacgg acgcgccgtt cctggcgccg gtgcccaacc gcggcaacag caacgtgccg
      421 gcgtgggtcg cgcacaccgc ggcgctggtc gccgaggtgc gcggcgtgcc cgccgccgag
      481 ctggcggagc tgacgacggc gaacgcccgc cgcgtgttcg ggctcgcgga gcgggactga
      541 cgtgggcgcc tccggcggcc ggccgggcgc gggcgaccgc agccgccccc cgcggccgca
      601 cgcgctgcgg cgctacggcc agaaccacct ggtggacaag aacgtgctgc acgccatcgt
      661 cgaccaggcc gcggtgagcc ccggcgacgt ggtgctcgag gtcggggccg ccggcggcct
      721 gctcacgcgg ccggtgctcg agcgcgcccg cttcgtgcac gcgttcgaga tcgaccgccg
      781 ctggctcccg cggctcgagg agctcgccgc cgccgagccg cgcttcgcgg tccacgccgg
      841 cgacgcgctc aaggccgacc tcggcgcgct cgagccgccg ccgacggcgc tggtcgccaa
      901 cctcgcctac aacatcgcca tcccgctgat catgacgacc atcgcggaac tgccctcggt
      961 gcggcgctgg gcggtcatgg tccagcgcga gctcggcgag cgcctgttcg ccgtgccctc
     1021 caccaaggcc tacgcggcgg tctcggtgct cacgcagctg gcctgccggc tggagaaggc
     1081 gcggccggtg cccgcctccg cgttccggcc gcgcccgcgc gtggagtcca gcttcgtgac
     1141 cttcgtgcgt cgcgagaccg aggacgacgc cgatggcggc actccggacg ccgtcgcggc
     1201 ggcgcccggc cccgccgagt acgccgcggt ctcgcgcctc gtgcggctgg cgttcggcca
     1261 gcgccgcaag acgctcgtca actcgctcgg cggcgccgcc cacgcgcacg tcacgctgag
     1321 ccgcgacgac gtgcgcgctg cgctgcgcga cctgggactg agcgaggcgg cgcgtccgga
     1381 ggagctcgct ccgccgcagt ggctgacgtt cgcgcgcacg gtcggccgtg cgtgacgagg
     1441 ccggcgacgg gcgtcgagtc ccgggggatc cggcctgatg ggaggaggcg acgatgacga
     1501 gtgacggcgt agcgctcgag ggggaccgcg ccgaggtcgt ggcgccggcc aaactgaacc
     1561 tggcgctgct ggtcgggccg gtgcggccgg acggcttcca cgagatcgcc tcgctcatgc
     1621 tgccggtgtc gctggccgat cgcgtgacgg tggagaggac gccgggcgcc gggctggacg
     1681 tcgtgtgcga cgtcgccccc ggcgccgaca acctcgccgc caaggtggtg cgcgagctcg
     1741 agcagcggct ggaccgctcg ttcgacgtcc gcgtgaccat cgacaagcgg gtgccgcacg
     1801 gcggcggcct gggcggcggc agctccgacg ccgccgcgac gctgatcgcg ctcgagcgcc
     1861 tgtacgacct cgacctctcg tcgcgggtgc gctacgaggc cgccgcggcc gtgggcagcg
     1921 acgtcccctt cttcctctgg ccgggcgcgc agctcgcgat ggggcgcggg caggtgctga
     1981 aggcggtgca gctgcccgcc ctgcacctgg tcgtcgccat gccggacctc ggcctcagca
     2041 ccgccgcggt gtaccgctgg cgcgatgagg acgcggaggt gacgctcaag gagttcgcgt
     2101 cgcgggcgag gctcctctcg agcaggatcc aggcggctgc gactcccggg gacgtcgccg
     2161 cgctcgtcgc caacgacctc gaggcgagcg tggtcgcgcg caaggagaag gtgcgcgagc
     2221 tcctcgagcg cctgcgctcg gcaggagcgc tcgccgcggc catgaccggc agcggcgcgg
     2281 cctgcttcgc gctcttcgac ggcgagctcg aggcgctgag ggcgcgggag gcggctgcgc
     2341 ccgcccgcgc ctggtacgtc accgacctgc agcccgaggg gcgcacgaag ccggccccga
     2401 ggcgcgggcg cggcaaaccg cctgcgtgat acattaacct gccgcttcgc ggcgggcggc
     2461 cggccgcctc gcggcgcccc ggccgtcagg cgctggggcg tagccaagtg gtaaggcacc
     2521 gggttttggt cccggcatcc ggaggttcga atcctcccgc cccagccagt gtcaagccac
     2581 gcgagagccg gcgggccccg cccccggcga catccccaag gtgaaccaga tgaccacagc
     2641 agatacgaag cgcccgccgc tcgccgtcgt gatcctggcg gccggcctcg gcacgcgcat
     2701 gaagtccgag gtgcccaagg tgctgcacga ggtgtgcgga cggcccatgc tctcgtacgt
     2761 gatcgacgcg gcgctcagcg tctcgcccga gcgcgtcgtc gtcgtcaccg gcccggagca
     2821 ggaggcgacc gccgagatcc tgccggtcgg ctgcgagcgc gccgtgcagc aggagcggct
     2881 cggcaccggc gacgccgtgc gcgccggcct ggagcctctg cacgacttcg agggcgacgt
     2941 catggtgctg tacggcgacg tgccgctggt cgacggcgac ttcgtcgccg gcctccacca
     3001 gcggcatctc gaggcccgcg cggccgccac gctcacgacc gtggagctcg acgacccggc
     3061 ccactacggg cgcgtcgtgc gcgacgagac cggcggcatc cagcgcgtcg ttgagcacaa
     3121 ggacgcgtcc ggcgaggagc gccagatcga cgagatcaac gtcggcttgt acgtcttccg
     3181 cagcgacgcg ctgcggccgg ccctggaccg cctcggcagc gacaacgccc agggcgagct
     3241 ctacctcacc gacgccgtgc acctgctgct ggacgccgag ctcacggtcg ccaccttcga
     3301 gagcgacgac gaggagatgt gcatgggcgt caactcgcgc gtcgagctcg cggtcgtcaa
     3361 cgcggcgatg cgccggcgcc tgctcgagcg gctcatgctg gcgggggtca ccgtcgagga
     3421 ccccgagacg acgtatgtgg attacggggt cgaggtcggc cgcgacacgc tcatccgcgc
     3481 caacacgcac ctgctggggc gcaccaccgt cggcgcggcc agtgaggtcg gcccggggag
     3541 cttcctgcgc gacgccttcg tcggcgaccg cgcgcgggtc gtcgcctcgc acctctacga
     3601 gtgcgtcatc agctctggct gcagcgtggg accgttcgcg tacatccggc ccaacacggt
     3661 gctcgccgag ggcgccaagg ccggcacctt cgtcgagatc aagaacagcc gcatcggcga
     3721 gcgcagcaag gtaccgcacc tcagctacgt cggcgacgcc atcgtcggcg tggacagcaa
     3781 catcgccgcc ggcaacatca ccgccaacta cgacgggtac gacaagcatc ccacgtacgt
     3841 cggcgaccgc gtgcgcaccg gcagcgacac gacgatcgtg gcgccggtca ccatcggcga
     3901 cgacgcgttc acggcggccg gctcggtcat cacgcgcgac gtgccgccgg gagcgctcgg
     3961 cgtcgcgcgc gagcagcaga agaacatcga gggctacgcc gggcgccgcg ccgctcgccg
     4021 gctgcgcgag caggagacct ccggataggg accatcacgg ccggcgcgct cgcgctttgg
     4081 tacaatcgtg gactggtccg gcggcagaaa cggaagagag gcgaacggtg gcggacagca
     4141 cctgctgcgt gacctcgccg cgcaagcggc tcatgatctt ctcgggacgc agcaacccgg
     4201 tcctcgcggc gaagatcgcc gacaagctgg acgtcgcccc cggcagcgtc gagctcaaga
     4261 ccttctccaa cggggagatc tacgcccgtt tcgaggagaa cgtccgcggc tccgacatgt
     4321 tcatcgtgca gtcgccgtgc gacgacatca acggcgagct catggaactg ctcatcatga
     4381 tccaggcggc gcagctggcc tcggccaagc gcatcaccgc cgtcatgccg tactacccgt
     4441 actcgcggca ggacaagaag agcgcggccc gcgagcccat caccgcgcgc ctggtcgcca
     4501 cgctgctcga ggcctccaag gtggaccgcg tcctgtccat ggacctgcac cagggccaga
     4561 tacaagggtt cttcgagatc ccggtggacc acatgacggc ggtgcccatg ctggcggact
     4621 acttccgcta caaggacttc ggcgacaccc cgctcgtcgc cgtctctgcc gacgccggcg
     4681 gggtcaagct cgccaagcgg ttcatcaacc gcgtcccggg gtgcgacctg gccgtgctca
     4741 cgaagatgcg ccccgagcac aacgtcgccg agactctgca cttcatcggc gacgtgcacg
     4801 acaagatcgc catcatcgtg gacgacatca tcgacaccgc ggggtcggtc acgagcgcga
     4861 tcgacacgct gtacgacagg ggcgccaagg aggtctacgt caccgcgacc cacggcatct
     4921 tcagcgggcc ggcctgcgag cgcatccggc agtccccggt caaagaggtc gtggtgaccg
     4981 acaccgtgca catcaagctg ggcctcgacg accccaagat caaggtgctg tcggtggccg
     5041 acatcctcgc ccgcaccatc aggaacgtgt tcgaagacga gtcggtgagc gagatcttcg
     5101 ccggcgacaa tcagctgttc taggagacca cacatggaca acgtcagcat cgtcgtgcac
     5161 aagcgcgacg tcaccggttc acgcgccgcc cggcgcatgc gcaagtccgg gctcatcccg
     5221 ggcgtcatct acgggcacgg caaggacgcc gtcctcatcg ccgtcgagcc gcacgcgctg
     5281 cgggaggcgc tcaccacgag cgccggcacc cacgccgtcc tcgacgtcac cttcgagggc
     5341 cagaagcgcg gccacaaggc catcgtcaag gagctcacgc tcgaccgggt caagagcaac
     5401 gtcgtccacg tcgacctcca ggagatccgc ctggacgaga ccatcgagac cacggtcgcc
     5461 atccgcttcg agggcgaggc caagggcgtc aaggccggcg gcatgctcga cgaatcgacc
     5521 cgcgaggtca ccgtcaaggg cgtcgtcacg gccatccccg agcacctcgt ggtggacgtc
     5581 agcgagctcg acatgaacga gacgctccag gtcgccgacc tgcaggtgcc cgagggcatc
     5641 gaggtgctgg ccgaccctga ggacgtcctc tgctcggtgc tgccgccgcg caaggtcgag
     5701 gccgaagaag gcgccgaggg cgaggaggcc gcagtggccg aggccgctgc cgccgagccc
     5761 gagctcgtgg gcaagaagga agacgaggcc gagggctgat cgccctgcgg cgggccgcct
     5821 gaccgcgagg catcgtggcc gtgttccgcc cgtcagcgag cgtcgagcgc ctcgtcgtcg
     5881 gcctgggcaa tcccgggccg gcgtacgagg gcacgccgca caacatcggc taccgcgtga
     5941 tcgaggagct cgcgcgccgg cacggcatcc cgcgtgccag gcgggggttc gacgggcgcg
     6001 tggccgaagg catggtccgc ggccgcacgg tgctgctgct gcagccgacc accttcatga
     6061 acgactcggg ccgcagcgtg gccgccgcgc tgcgcgcggt caagctgcgg ctcgaggacg
     6121 tgctcgtggt gcacgaccac atcgacctgc ccttcggccg cctgcggctc atggagggcg
     6181 gtggcagcgg gggtcacaac ggcctcaaga gcatcaccgg gctgctcggc gccggctacg
     6241 cgcgcttgcg cgtcggcgtc gaccggccgc cgagcacgga ccccgacgtg gtcgccgagt
     6301 acgtgctcag cccgttccgg gagagccgcg cggaggtcga cagggtgacg gcggcggcgg
     6361 ccgacgcggt cgagctatgg ctggacgccg ggcttgaggc cgccgccaac gccgtgaacc
     6421 gtctcgactg ctgagacgga gtgctggcgg agcaccggcg tcaccgccag cagcggctcc
     6481 ccgggcagcg tcgacctggc gagggccacc acgatggcgt cctgcgccct ggtggcccgc
     6541 tcccagtcca gcgacccgcc gttcatgagg atcgagaacg cgaccaggtg gtcgttggcg
     6601 ctcgtgacgt acccggagag gcagatggcg acgttgagcg tgcccgtctt gacccgcgcg
     6661 ttgccggcgg ccgcggtgcc gcgcatgcgg tcgtggatcg tgccgtcctc gccggcgatc
     6721 gccagcgagt cgtagaagtc gtcgaagtcg tcgcgctggc gcaaggcgcc gagcaccttg
     6781 accacgccct cggcggtcat ccggttgccg tagctgagcc cggagccgtc ctggatcacg
     6841 taggtgccgg tctttacccc gagcgcgtgc agggcggcct tgctcgcccg ggtgccggcc
     6901 gccgtgctgc cgtcgccgtg gaactccttg ccgagcccct tgagcagcat ctcggcgaag
     6961 gcgttgtcgc tctccttgtt catgtgcttg aggatgccgc ggagcgccgc cgagtactgc
     7021 cggtcgacga ccttcgcggt gtccggtacc ttggccgtcc ccgacgggcc cgtgacccgg
     7081 acgccgacgt tcgcgagcgc ctgcttcatc agcttggctg cgtaggtcgc cggagcgctc
     7141 acccggttgc cgtcgcggag cccctggttg ccggagaggg cggagagcgg gccgcactcg
     7201 agctgcaggc cgtccttcca cgtcggcacg gtcctgagct tgtcgaacca ggaggcgtcg
     7261 ccgaggaccc tgccccggac cttcctgacg ccctgacgtt tgagggtcct ggcgaaggtc
     7321 tcgaacgacg cggtcgtcat gccgaacacg tcctgctggt aggcccgagt cgagaggctc
     7381 gggtcgccga gcccccggag gtagaggtct ccctgcagca ccccgccggc ggcgaccggc
     7441 acgtcctcgg cgcagagctc ggtgacgaag cggtggtcga cgccccagtc caggatcgcg
     7501 gccgcgctgg tcacgatctt gaggttggac gccggcgtca ggctggtgcc ggcgttgagc
     7561 tcgtagaccg ggagcgcggc gtcgaggtcc cagacgtaga ctccggtgcc cgcgccgtgc
     7621 agcccggctt gccgcaggga cgcggcgatg gcgccgtcga cgccggcgac ggcgggagcc
     7681 gggcagaggg ctcccgcgca ggcggtggcg aggaccagcg cgaggagcag gaggaaggcg
     7741 tgcgtgcgcc ggatccgtgt gccccgggcg ccggcccgcg ccgggagcgc atctgcgatg
     7801 actgtccctc ccatcctggg cctcatatta cgttgcgccg cgccgccggg gaacccggct
     7861 tgactcacct cgccgtcccg ggggataaga gaggcatgag cctcctcgtg cactcaggcg
     7921 cctcgtgatg cggcgcgcga gaacctgcct tctgctggtc gtcgcgctgg cctcgctggc
     7981 cgccctgtcc ggcggcctcc cggagggcac cgccggcgcc gccacggcgc cggccgcgtt
     8041 cgccggggcc gcgttcgcgg cctcgtcgcc gggcgcgcct ccctgggaga ccgccggtcc
     8101 gtacccggcg gcgttcgacc tgcgcgcgga aggccgcctg acccccgtgc gctcacagga
     8161 cagctacagc acctgctgga tcatggcggc catgggctcg ctcgagtcgg tcgtgctccg
     8221 caaggagggg ctgcccctcg acttctccga gaacaatctg gccaatcaca tgtcctcgcg
     8281 gctcgacttc gaaggcatgg cgcccagcga gctggcgctc gcctactacg cccgctggga
     8341 gggcccggtg ggggagtcgt cggacccgta tccgcgcccc ggcaagtcgc cgccgtacct
     8401 gcgcgccgtg cgccacctgc aggaggcgct cttcctgccc gagcggcagg gagcgctgga
     8461 caacgacgcc gtcaagtggg cggtcatgac ccacggcggc gtggacgcgg ccgtcgactt
     8521 ccgcgtgcag gacgagttcg agtcgtggaa cgcgtcgacc ttcgcctact acaacgcctc
     8581 gcgcagcacg ccgaaccacc acatcctcgt agtgggctgg gacgacgcct acccggcctc
     8641 caagttcctc gcggggtcga ggcctcccgg cgacggcgcg ttcctcatca agaacagctg
     8701 gggcgcagac ttcgcggacc atggctacct gtgggtctcc taccacgact caaccatcgg
     8761 ggacgcgctg gtggtgttct cgggcgtcga gcccgcgggc aactacgacg ccgtctacca
     8821 gtacgacgcg ctcggccgca gccgctggat cacggccggc ggcggggagc aggcgtggtt
     8881 cgccaaccgc ttcaccggcg ccggcgccgg tcgcgtggcg gcggtctcgt tctacgcgcc
     8941 cgtgcccggc acgacgtacc aggtgcgcgt cgcgcgcgcc ctgcagggtg tcgctgccgc
     9001 cccggtcgcc gccgccgggg tcctctccgt ggcggggtac cacacggtgc gcctggagga
     9061 gccggccgcg gtgacggcgg gggagaagtt cgtggtcgcc gtccgcgtga cgacgccggg
     9121 ctgggcggag cccgtgcccg tggaggcgcc gtccggcctc atctctccgc gggcgcgcgc
     9181 ggggcagtcg tacgtgagcg ccgacggtgc ggcgtggact gacctgacga gccacacggg
     9241 gctgtcgcgc gccaacgtgt gcctcaaggc gttcgtggac gccgccggcg tcgccgacag
     9301 gcgggccccg cgagtggaga tccgcggcga cgtggtgcgg cgcggcgcgc aggcggtgat
     9361 cccctggcgg ctcacggatc cggccttctc cagcgcgagc gccatcgtcg agctcagcct
     9421 gcgcgacgcc gccggcaggc tgatcgccca gcggcgcatc cccgccgtgg cggtgggcga
     9481 gcgtggggtg tgggcgctcc gggcgagctg gccgcgcggc cggtacagcg tgcgcggccg
     9541 cgcctacgac gtggcgggcg gccgccaggc gacggcgagc cgcaccgcgg tggtcgtcgg
     9601 cggcgtagcg tcggccgcac cggcggtccc ccggcgctga cgccggcatc cggccggcgc
     9661 ggctccggcg ggcgctcctg ccggcgcgcc gtcggctaga atgggcgagg ccccgccgcc
     9721 gtgaggcgcg cggggcgttc ggcgtgcccg gaggaggtca agtgtttctc gactacctgc
     9781 tgaacgacga gaagttcgag gacgccgcgc cgcggctgcg gcgcggcgcg ggtgcgtacg
     9841 cgccgagctt ctacgcgccg tacgtcctcg ccgccgccct ggcgggcggc ggcggccacg
     9901 tcgtcgtcgc acccgacggc gaggcggcga cgcggctcgc cgccgatctg tccgtctacc
     9961 tcgacgtcgc cgtgccggtg ctgccggcgc gcggcgtgct gtacggcgcc gacgtggcgc
    10021 cctcgccgca cgtggtcggc gagcgccagc aggcgcgcac gacgctcgcc gccggcggcg
    10081 tggtcgtcgc cgaggccgtg gcgctgctcg agcggttcct gccgctccag ctccagccgc
    10141 ggcccttgac cctgtctccc ggcgaggaga tcgacttcga cggcgcggtc gcgcgtctcg
    10201 ccgcgctcgg ctaccagcgc atcgagcagg tccgcgcccg cggcgggttc gccgtgcgcg
    10261 gcggcctcat cgacgtctac cccgcgctcg gcgaccccct gcgcgtggag ttctggggcg
    10321 acgaggtcga gcgcctgcgg accttctcca tctactcgca gcgcacgacc ggcgagatcc
    10381 gggcggcgac cgtgttcgcc gcgttcgagg ccgacacctc ccagccggag tacgtcaacg
    10441 ggctgcacca ggccgtggcc gagtgggagc gggagggccg cgaggagacg ccggaggagc
    10501 tcgagcgccg cgcggccgtg cgcgcgctcg cggcgcttgc cggccgtttc accacgctcg
    10561 gcgagcaggc cgctgcggcg ggcgccggct gggccgtctt caatcccgac gagacctacc
    10621 gcgcgctcgc cgacttcgac ggcgaggtgg cgacggcggt gccgggcgcg gctctgcgcg
    10681 agcggctcta cgcgccgctc gccgacgccc gcgccctgct cgccaaggcg ctccgcgtcg
    10741 acgtcgtgca gcgcgaccag ccgctccagt tcgccgcctc gcggccgcag ttcgcggcgc
    10801 gcgacctcgc cagcgccgag cgcgacctca tccgcctggt caacgacgac taccgcgtct
    10861 tcgtcgtctt ccgccacgag ggcgaggccg agcgcgccac gttccgtctg cgcaacctct
    10921 ctgccgaggt cgtgacgccg gagcagctgg cccggctggg agaagcgggg ccgggcctct
    10981 acttcgtcgc cgcgcccctg cgcgacggct tcgtggcgga ggacctcaag ctcgccgtgg
    11041 tcaacgagcg cacgctcatc cgttcggcgc cgcgtgagcg ccgcttcgcg gccggcacgc
    11101 ggctcaccac cttcttcgac gtgcgcccgg gcgaccacgt ggtccacgag gaccacggca
    11161 tcgcccgctt cgccggcatc gagacgcgca ccgtcgccgg cgtcacccgc gactacctgc
    11221 tgctcgagtt ccgcggcgac gaccgcgtct tcgtcccgca cgaccagatc ggcaaggtca
    11281 gccgctacgt gggcgcgtcg ggcgccacgc cgtcgctcga caagctcggc ggcacgcact
    11341 ggcagactgt caagacgcgg gcacgcacgg ccgtggtcga gatggcgggc gagctgctgc
    11401 agctctacgc cgcccgccag gccatccccg gcttcgcgtt cccgcccgac ggcgagctca
    11461 tgcgccgcct ggaggaggcc ttcccctacg aggagaccga cgaccaggcg gaggccatcg
    11521 acgaggtcaa gaacgacctg gaggcgccgc acccgatgga ccgcctcatc tgcggcgacg
    11581 tcggctacgg caagacggag gtcgcgctgc gcgcggcgtt caaggcggcc gaggccggca
    11641 agcagacgct cgtcctcgtc cccacgacca tcctcgccga gcagcactgg atgacgttcg
    11701 aggagcgctt cgccgacctg ccggtcacgg tgggcatggt ctcccggttc cgcagcggcg
    11761 ccgagcagcg caagaccctc gccgccttca gccggggcga ggtcgacgtg ctcgtcggca
    11821 cgcaccgcct gttgagcacg gacgtgcggc ccaaggagct gggcctggtc atcgtcgacg
    11881 aggagcagcg cttcggcgtg cgccagaagg aactcctccg caacctcaag ctccaggtgg
    11941 acgtgatgag cctctcggcc acgcccatcc cgcgcaccct gcagatgagc ctgagcggca
    12001 tccgcgacat ctcggtgatc gagaccgccc cgcgcggccg gcacgagatc cgcacttaca
    12061 tcggcgagta caaggacgac ctggtgcgcg tcgccatcga gaaggagctg gctcgcgagg
    12121 gccaggcctt ctacctgcac aaccgcgtgg agacgatcga ccaggcggcg gagcacgtgc
    12181 gcgcgctcgt gccccgggcg cgcgtcctgg tggcgcacgg gcagatggcg gagcgcacgc
    12241 tcgagaaggt catgctcgcg ttcctcgccg gagaggcgga cgtgctggtg acgacgtcga
    12301 tcatcgagag cgggatcgac atcccgaccg ccaacacgct cgtcgtggag cgcgccgaca
    12361 tgctcggact ggcgcagctg taccagatcc gcgggcgcat cgggcgcagc gacgcgcacg
    12421 cgttcgccta cctgctctac ccgagcgacg acctgctgac gagcgaggcg gccgcgcgtc
    12481 tgaccacgct cagcgaccac accgacctcg gcgccgggtt caagatcgcc atggccgacc
    12541 tcgagatccg cggcgccggc aacctgctcg gcgacgagca gagcgg
//
LOCUS       DASUMX010000274         3477 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_101042, whole genome shotgun sequence.
ACCESSION   DASUMX010000274 DASUMX010000000
VERSION     DASUMX010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3477
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101042"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..945
                     /locus_tag="VFZ86_13075"
     CDS             121..945
                     /locus_tag="VFZ86_13075"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX5643250.1"
                     /translation="MSTPRTIVISDADGYPELIRKALTHSGFRAGEDVLVYAGDFVHR
                     GPDAAECVATVEECGPLADSPLDPQPGEVVVLWGNHDIAVLRGTFAYPNSPPADGLRP
                     LFRRRFDDGAWRLAACVGDVLITHAGVSTEYAADWQACGYDPKRLAARLETEFRNCVA
                     YLLQPGAARANPPLLGNLGPLGYRPSLEPRDKLLPGLVQVAGHTPGGGVTARSLRAAG
                     MFMVDPDVLRGLRPEDRGRYRHAVIEEGVVRIEEGDLNTTASASVSSAVRPDRRLS"
     gene            complement(913..1680)
                     /locus_tag="VFZ86_13080"
     CDS             complement(913..1680)
                     /locus_tag="VFZ86_13080"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEX5643251.1"
                     /translation="MSDGRQRIQQVLDGARAEGRAALLVCLPSSQPTAWLVAAAKACT
                     AANADLLEFQVHQPRHPAEVVEAIAVVAREVSPPCLLWTDFPVVRDFALTEAQPWRLV
                     PECVRAGIAGVAAPVGSRYTSAFAAACGEDLARIPFVSPDMPPDDLERVCGHDPAFVY
                     AIGVSTSPPTDAGVFEGVAEYLERVRQIAGAPVFLGAGVGTPAQAALAATFADGVAVA
                     KAAFQTLGRASAEGRDERAALAALVRELRQASVRAHG"
     gene            1906..2211
                     /locus_tag="VFZ86_13085"
     CDS             1906..2211
                     /locus_tag="VFZ86_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017841698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX5643252.1"
                     /translation="MKKLRKTESFARWLDDLRDLRARARIQVRIERLAAGNPGDVKAV
                     GKGVSELRIDYGPGYRVYFTRREGGVIVLLAGGIKSTQTADIENAQRLADGLREGHR"
     gene            2208..2549
                     /locus_tag="VFZ86_13090"
     CDS             2208..2549
                     /locus_tag="VFZ86_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013032508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antidote protein"
                     /protein_id="HEX5643253.1"
                     /translation="MRRTVTTSYDVADHLRTPEEMAAYLEASLEEAQGDAAFVAKALG
                     DIARAKGMTQVARDTGLSRESLYKALSGARSPDFDTILRVVGALGLQLHAGAARRCEP
                     IGSSRRTRSTC"
     gene            2782..3273
                     /locus_tag="VFZ86_13095"
     CDS             2782..3273
                     /locus_tag="VFZ86_13095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX5643254.1"
                     /translation="MLIGVLSDTHGRLDPAIEEVFAGVDLIVHAGDVGDEAILARLSA
                     IAPLTAVRGNCDDAPWGLALPCVASEVLDGVRVAVVHDREMLDVYASPALREAMSGGV
                     GVLIHGHTHCAEVTREQDTLGLNPGSASLPHYELPRSVALLRIEDGDCSVEIRGLGHD
                     LLL"
     gene            3284..>3477
                     /locus_tag="VFZ86_13100"
     CDS             3284..>3477
                     /locus_tag="VFZ86_13100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643255.1"
                     /translation="MKLLLCSGCHDVVALQMDSRRTCKCGSSSGQYRADGYDADVWGP
                     RAVMLGLRNQYLPALVLGQGA"
BASE COUNT          528 a         1214 c         1226 g          509 t
ORIGIN      
        1 gtcctcggcc acaaccggct cgactgcagc aatgcgcggg aagtggtgaa ccgtgccgca
       61 caggagtacg caacggtcta gacgggctcg ccgccgcgac gcgcctcaag gtccccactt
      121 gtgagcaccc cgcgcaccat cgtcatcagc gatgccgacg ggtaccctga gctgatccgc
      181 aaggccctca cgcacagcgg gttccgcgcc ggcgaggacg tgctggtcta cgccggcgac
      241 ttcgtccatc gcggcccgga cgcggcggag tgtgtggcga ccgtcgagga gtgcggaccg
      301 ctcgcggact cgcccctcga tcctcagccc ggcgaggtgg tcgtcctctg gggcaaccac
      361 gacatcgccg tgctgcgcgg gacctttgcc tatcccaaca gcccgcccgc cgacggactg
      421 cgcccgttgt tccgtcggcg gttcgacgat ggcgcctgga ggcttgccgc ctgcgtcggc
      481 gacgtcctca tcacgcacgc cggcgtctcg accgagtacg ctgccgactg gcaggcctgc
      541 ggctacgatc cgaagcggct ggcggcgcgt ctcgagacgg agttccgcaa ctgcgtcgcc
      601 tacctgctcc agcccggcgc cgcacgcgcg aacccacccc ttctcggcaa cctcggcccg
      661 ctcggctacc gcccctcact cgagccccgg gacaagctcc tgccgggact cgtccaggtc
      721 gccgggcaca cgccgggcgg gggcgtcacc gcccgcagcc tgcgcgccgc cggcatgttc
      781 atggtggacc cagacgtgct cagaggcctt cggcccgagg accggggccg gtaccgccac
      841 gcggtgatcg aagaaggagt cgtgcggatt gaagagggcg atctgaacac gacggctagt
      901 gcgtcggtct cgtcagccgt gcgcccggac cgacgcctgt cgtagctcgc gcaccagcgc
      961 agccagcgcc gcgcgctcgt cgcgaccttc cgcggatgcg cgcccgagcg tctggaacgc
     1021 ggctttggcg accgcgacgc cgtcggcgaa cgtggccgcc agggccgcct gcgccggcgt
     1081 gccgacgccg gccccgagga agacgggcgc accggcgatc tgacgcactc gctcgaggta
     1141 ctccgcgacc ccctcgaaga cgccggcatc cgtgggtggg ctcgtgctca cgccgattgc
     1201 gtagacgaac gccgggtcgt ggccgcagac ccgctcgagg tcgtctggag gcatgtccgg
     1261 cgagacgaac gggatgcggg ccaggtcctc gccgcaggcg gccgcgaatg cggatgtgta
     1321 ccggctgccg accggcgccg ccacgccggc gatgccggcc cggacgcact ccggcaccag
     1381 ccgccacggc tgggcttcgg tgagggcgaa gtcgcgcacg acggggaagt cggtccacag
     1441 aaggcagggc ggcgacacct ctcgggccac gacggcgatg gcctcgacga cctccgcggg
     1501 gtggcgcggc tggtgcacct ggaactcgag caggtcggcg ttcgccgcgg tgcaggcctt
     1561 ggcggcggcg acgagccacg ccgtcggctg gctcgacggc aggcacacga gcagcgcggc
     1621 gcgcccctcc gctcgggcgc cgtcgaggac ctgctggatg cgttgtcggc cgtcggacac
     1681 gacgggaagc ctacgcgccg gaggtgacgg aacggggtag cggtctgcaa cgtccaccac
     1741 gcgagtcgct cgtcactctc gttggcggat cgtcggccgt ctacgctcgc agccatgaac
     1801 gagcgtctgc cgattgctca ccttgccccc ggcgtcaccg tactgcccag tcggcgccgg
     1861 ccgctgccgt cttgacgaag tgtagcctag tggctacagt tgccattgaa gaagctccgt
     1921 aagaccgagt cgttcgcgcg ctggctcgac gacctgcgcg accttcgagc aagggcccgt
     1981 atccaggtca ggatcgagcg cctggctgcc gggaaccctg gcgacgtgaa ggcagtgggg
     2041 aagggagtct ccgaattgcg catcgactac ggtccagggt acagggtgta cttcacgaga
     2101 cgggagggtg gtgtgatcgt tcttctggcc ggtggcatca agagcaccca aacggcagat
     2161 atcgagaacg cccagcgtct ggcagacggc ctgcgggagg gtcatcgatg agaaggaccg
     2221 tcaccaccag ctacgacgtc gccgaccacc ttcgcacgcc ggaggaaatg gccgcctatc
     2281 tggaagcgag cctcgaggag gcccagggcg atgccgcgtt cgtcgccaag gctctgggcg
     2341 atatcgcccg cgccaagggg atgacccagg tggcgcgcga caccggcctc tcgcgcgaga
     2401 gtctctacaa ggcgctctcc ggcgcgcgca gtcccgactt cgacacgatt ctgcgcgtgg
     2461 tcggcgcctt ggggctccaa ctgcacgcgg gagccgcgcg ccggtgcgag cccatcggct
     2521 catccaggcg tacgcgcagc acttgctaag tcgccggctc gtgctcccgg tgtcaggggc
     2581 gacgggcgac ctacacgatg acaggtcgcc cgttctttgc gctcagcgag gtcgtgctcg
     2641 caagcccgtg ggacccctca tccctcttca cgttcgccgc gcgcgtgccg ataggcagta
     2701 ccgtgaagaa aacggcgccc ttgagggcgt ggcaagagga gatcgcggct tggtgacaga
     2761 cgtatcgaag gactcgcgcg catgctgatc ggcgtcctca gcgacacgca cggtcgtctc
     2821 gacccggcca tcgaggaggt cttcgccggc gtggatctca tcgtccacgc gggagacgtg
     2881 ggcgacgagg ccatactcgc ccggctcagc gccatcgcgc cgctcacggc ggtgcgcggg
     2941 aactgcgacg acgcgccgtg gggcttggcg ctgccctgcg ttgcgagcga ggtgctcgat
     3001 ggcgtcaggg tggccgtcgt ccacgaccgg gagatgctcg acgtctacgc gtccccggcg
     3061 ctgcgcgagg cgatgagcgg tggcgtcggc gtgctgatcc acgggcacac gcactgcgcc
     3121 gaggtcaccc gcgagcagga caccctcggt ctcaaccccg gctccgccag cctgccgcac
     3181 tacgagctgc cgcgctcggt cgcactgctg cgcatcgagg acggtgactg cagcgtcgag
     3241 atccgcggac tcggacacga cctgctgctg tgagcatgat cgcgtgaagc tcctgttgtg
     3301 cagcggctgt cacgacgtcg tcgcgctgca gatggacagc aggcgtacct gcaagtgcgg
     3361 gtcgtcgagc ggccagtacc gcgccgacgg ctatgacgcc gacgtctggg ggccgcgggc
     3421 ggtcatgctg gggctgcgca accagtacct cccggcgctg gtgctgggcc agggagc
//
LOCUS       DASUMX010000275         6233 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1250164, whole genome shotgun sequence.
ACCESSION   DASUMX010000275 DASUMX010000000
VERSION     DASUMX010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 6233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6233
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1250164"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1514)
                     /locus_tag="VFZ86_13105"
     CDS             complement(<1..1514)
                     /locus_tag="VFZ86_13105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014248330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEX5643256.1"
                     /translation="MGTETGAPQPGAAEGWRFWIDRGGTFTDVVARPPGGGLRTLKLL
                     SEDPARYEDAAVEGVRRLLGLEAGEPVPAARIADVRMGTTVATNALLERAGSPTLYVT
                     TRGLGDALRIGYQDRPDIFALDIRLPEPAYARVLEVDERLGADGTVVRALDEDGARRG
                     LEEARRAGLDAVAIALIHGYAHPEHERRVAELARVAGFSQVSVSHETSPLMKLVGRGE
                     TTVVDAYLSPILRRYVDGVAAQLGDVRPRFMQSHGGLTDARLFRGKDAILSGPAGGVV
                     GAVAVSRRAGFDRVIGCDMGGTSTDVSHYAGELERSYESVVGGVRLRAPMLRVHTVAA
                     GGGSICAFEAGRYRVGPRSAGADPGPACYGKGGPLTVTDCNLVTGKLRPAFFPRVFGP
                     EGAAPLDEEAAREALAAVAASMRAAGVEPPPPEQLADDFVRVACEAMARAIRRISTQR
                     GHDVTGHALCCFGGAAGQHACLVADALGMTQALLHPLAGVLSAYGMGLADVRALR"
     gene            1574..2080
                     /locus_tag="VFZ86_13110"
     CDS             1574..2080
                     /locus_tag="VFZ86_13110"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5643257.1"
                     /translation="MDLQEDTERHMAISIRRLGPGDEGLLESLAGEADDFDLPGASAP
                     EPPLPPDEATAYLSDPSVLHWVAEEEGVLVGELLCHVLRLPSGEGRELLLYAVGVRSA
                     HRRRGVGAALVGEMLRWALEAGIRVVWVLADNPGAEAFYAACGFARGGEGEQGVLMLR
                     QVAGEEGR"
     gene            complement(2118..2441)
                     /locus_tag="VFZ86_13115"
     CDS             complement(2118..2441)
                     /locus_tag="VFZ86_13115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643258.1"
                     /translation="MIVMKGILVLSGSGPLLLLSSYPEIDDPLLIAKLRAKGLAKFLA
                     WEVPVDHLRDLYGYTFRDIVEELEARDDMRVLDFDGHRIFLNLSLLDLGQHILFEDQH
                     VLAER"
     gene            2731..3408
                     /gene="nth"
                     /locus_tag="VFZ86_13120"
     CDS             2731..3408
                     /gene="nth"
                     /locus_tag="VFZ86_13120"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEX5643259.1"
                     /translation="MGASSPAERLKQAADILDDVYGRPVLSPRYPPVDELVFTVLSQN
                     TADVNTERTFAALKARFPEWTAARDASAEEIEEAIALGGLAHTKAPRIKRILEAISER
                     TGAPDLSELDGMSDLEAQDYLVALPGVGPKTAACVLLFALQRPVMPVDTHVHRVARRL
                     GIIGEQVTADQAHPLLTELAGPGDAEQVYAVHVDLVRHGRRVCHARRPACAECPLAPM
                     CPSAGLV"
     gene            complement(3422..6145)
                     /locus_tag="VFZ86_13125"
     CDS             complement(3422..6145)
                     /locus_tag="VFZ86_13125"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX5643260.1"
                     /translation="MATSRDDLRATPSGLSTAEADRRRAAGQGNDVEIKAGRTYGRIV
                     RDNVFNFINNLFYFLGILLLILGKPLDAFAVVFVIGANTVISLFQEIRAKRVMDKMAI
                     LLRPKADVLRDGRLVEIDPSQVVLGDVLRLEPGDQVVVDGPMIGQGRMEVDESLLTGE
                     SDLVTKHPGDELMSGSFCMTGGGYYEAARVGMDSFANQLTAKAKVFKRELTPLQRQVV
                     QIVRILLIVVIVFEALVWIRNVAGGVPFVESVRMSTVILALIPNGLVLSIALTYALGA
                     VRLLGKDVLVQQFNAVESLSNVDVLCTDKTGTLTSGVVTFEELAGLGADDARAARLLG
                     AFAASTTDANRTIDALRTALPAERVPAAHEAFFSSERKWSGLAFADGEARGTYVLGAP
                     EVLSPALGEGAGDAWRAQTDAWAARGLRVVLFAGRQDPVAFSDDPDAPPVLPPGLEGI
                     ALICFSDELRPGVRGTLEEFSDAGIDVKIISGDNPKTVVALATQAGVQSLRRRVGLDS
                     YCDMVTRRVEEETTGAEPTAPEAAPADTPPRDLVAVSGAELAELSPEDFAEKADEAGI
                     FGRVTPEQKQDLVEALRDRGRYVAMIGDGVNDVIALKQANVAVAMQGGSQAARSVGDL
                     ILMKDTFAPLPFAFREGQRIINGMNDILRIFMVRIFFKAAMIAIVMGIGGFPFAPRQS
                     ALLSFFGATVPAIAFALWAQPGPTPKVGLFKLLARFVLPTVVFMTAIGTGVYLLWAVP
                     AKRAYLADHPGAGAKELIENAYPQAMTALTIFACFAAIFLILLIVPPNRWWSGGAAVV
                     RNDWRMVGVVGFLFACMAVVLAVPLAHTLFEITPLPWWQYFVLLGFAFGWSQLCLLVW
                     KGHLLDRWLGTAEDPGNVCAKAAAEAAAAGAAAEDAAATDAAASAEAAARR"
BASE COUNT          873 a         2229 c         2282 g          849 t
ORIGIN      
        1 cgcagcgcgc gcacgtcggc gaggcccatg ccgtaggcgc tgagcactcc ggccagcggg
       61 tgcaggagcg cctgcgtcat gccgagcgcg tcggcgacca ggcaggcgtg ctgaccggcg
      121 gcgccgccga agcagcagag cgcgtggccg gtcacatcgt gcccgcgctg cgtcgagatc
      181 cgcctgatgg cgcgcgccat cgcctcacag gccacgcgca cgaagtcgtc ggcgagctgt
      241 tccggcggcg gcggctcgac gccggcggcg cgcatgctcg cggccacggc cgcgagcgcc
      301 tcgcgcgccg cctcctcgtc gagcggcgcg gcgccctccg gcccgaacac tcgcgggaag
      361 aacgccggcc gcagcttgcc ggtcacgagg ttgcagtcgg tgaccgtgag cggcccgccc
      421 ttgccgtagc acgccggccc ggggtcggcg ccggcgctcc tcggaccgac ccggtaacgc
      481 ccggcctcga aggcgcagat gctcccgcct ccggcggcga cggtgtgcac gcgcagcatc
      541 ggcgcgcgca gacgcacgcc gcccacgacg ctctcgtagc tgcgctcgag ctcaccggcg
      601 tagtggctga cgtcggtcga tgtgccaccc atgtcgcagc cgatcaccct gtcgaacccg
      661 gcgcgccggc tcacggcgac cgcgcccacg acgccgccgg cggggccgga gaggatcgcg
      721 tccttgccgc ggaacagccg cgcgtcggtg aggccgccgt gcgactgcat gaagcgcggg
      781 cgcacgtcgc ccagctgcgc cgccacgccg tcaacgtagc ggcgcaggat gggggagagg
      841 tacgcgtcga ccacggtcgt ctcgccgcgg ccgacgagct tcatgagtgg gctcgtctcg
      901 tgcgagaccg acacctggga gaagccggcg acgcgcgcca gctcggccac gcgccgctcg
      961 tgctccggat gggcgtagcc gtggatcagg gcgatggcga cggcgtcgag gccggcgcgg
     1021 cgggcctcct ccagaccgcg ccgggcgccg tcctcgtcga gcgcgcgcac gaccgtgccg
     1081 tcggcgccga ggcgctcgtc cacctcgagc acgcgggcgt aggccggctc cggcagccgg
     1141 atgtcgaggg cgaagatgtc cggccgatcc tggtagccga tgcgcagcgc gtcgccgagg
     1201 ccgcgcgtgg tcacgtagag cgtgggcgat cctgcgcgct cgaggagcgc gttggtcgcc
     1261 accgtcgtgc ccatgcgtac gtcggcgatg cgtgcggccg gcaccggctc gcccgcctcc
     1321 agcccgagca gccgccgcac cccttcgacg gcggcgtcct cgtaccgggc cgggtcctcc
     1381 gagagcagct tgagcgtccg caggccgccg cccggcggcc gcgccaccac gtcggtgaac
     1441 gtcccgccgc ggtcgatcca gaaccgccag ccctcggcgg cgccgggctg cggtgctccg
     1501 gtctcggttc ccatcccggc gaggctacca cgccgggatg cgagctcgtg ctcgcccggc
     1561 cgcgactccg ggtatggacc tccaggaaga cacggagcgg cacatggcca tcagcatcag
     1621 acggctcggc cccggcgacg agggcctgct cgagagcctc gccggcgagg ccgacgactt
     1681 cgacctgccg ggtgcgtccg cgcccgagcc gccgctgccg ccggacgagg cgacggccta
     1741 cctcagcgac ccgtccgtgc tccactgggt cgcggaagag gagggcgtcc tcgtcggcga
     1801 gctcctctgt cacgtcctgc ggctgccgtc gggggagggc cgggagctcc tgctgtacgc
     1861 cgtcggcgtg cgctcggcgc accgccggcg cggcgtcggc gccgcgctcg tcggcgagat
     1921 gctccgctgg gctctcgagg cgggcatccg cgtggtctgg gtgctcgccg acaacccggg
     1981 agcggaagcg ttctacgccg cctgcggctt cgcccgcggc ggcgaaggcg agcagggcgt
     2041 cctgatgctg cggcaggtgg ccggcgagga gggccggtag gcccacacgc cggccacgcc
     2101 gccgcagcag ccgccgctca gcgctccgcc agcacgtgct ggtcctcgaa gaggatgtgc
     2161 tgcccgaggt cgagcagcga caggttgagg aagatccgat gcccgtcgaa gtcgagcacg
     2221 cgcatgtcgt cgcgcgcttc gagctcctcg acgatgtcgc ggaaggtgta gccgtagagg
     2281 tctcgcaggt ggtccaccgg gacctcccag gcgaggaact tggcgagtcc cttggcgcgc
     2341 agcttggcga tcagcagcgg atcgtcgatc tccggatacg agctcaggag caggagtggt
     2401 cccgagccgc tgagcacgag gatgcccttc atcacgatca ccccctcatg ggtcctcctg
     2461 atgaggattc ccgcggctgg ggcggtcgaa atgcgttcgg ccgggcggcc cggagtgcgg
     2521 cggccggtcg tgacgcgggg cacgggcgtg cacggcgtcc tctcagaacg tatccacatt
     2581 gtagatacgt tttggctggg acccgtccgt tgcaccccgt ttgtagagac attgtatcta
     2641 cgcgatcaga ggccccggcg agagcagtcc ggtccgcggg cggtcgccgg tgccggggga
     2701 cgacgatccg cggcggtata ctttcggccg atgggagcat cgtcacccgc agagagactc
     2761 aagcaggctg ccgacatcct cgacgacgtc tacggccgcc ccgtgctctc gccgcgctac
     2821 ccgccggtgg acgagctcgt cttcacggtg ctcagccaga acacggccga cgtcaacacg
     2881 gagcgcacgt tcgccgcgct caaggcgcgg ttcccggagt ggaccgcggc ccgcgacgcg
     2941 tccgccgagg agatcgagga ggccatcgcc ctcggcgggc tcgcgcacac caaggcgccg
     3001 cgcatcaagc gcatcctcga ggcgatcagc gagcggacgg gcgcccccga tctgagcgag
     3061 ctcgacggga tgagcgacct tgaggcgcag gactacctgg tcgcgctgcc gggggtcggg
     3121 cccaagacgg ccgcctgcgt gctgctcttc gcgcttcagc ggccggtcat gcccgtggac
     3181 acgcacgtgc accgcgtggc gcggcggctc ggcatcatcg gcgagcaggt gaccgcggac
     3241 caggcgcacc cgctgctcac cgagctcgcg ggaccgggcg acgcggagca ggtgtacgcc
     3301 gtgcacgtgg acctcgtgcg gcacggccgc cgcgtctgtc acgcccggcg gccggcctgc
     3361 gctgagtgcc cgctcgcgcc gatgtgcccg tcggccgggc tcgtctgacc ccggcgcgcc
     3421 gtcaccggcg ggcggcggcc tccgcgctcg ccgcggcgtc ggtcgccgcc gcgtcttccg
     3481 ccgcggcgcc cgccgccgca gcctctgccg ccgccttggc gcacacgttc cccgggtctt
     3541 ccgcggtccc cagccagcgg tccagcaggt ggcccttcca caccagcagg cagagctggc
     3601 tccagccgaa cgcgaacccg aggagcacga agtactgcca ccacggcagg ggtgtgatct
     3661 cgaacagcgt gtgcgcgagc ggcacggcga ggacgacggc catgcacgcg aacaggaagc
     3721 cgacgacgcc gaccatgcgc cagtcgttgc gcaccacggc cgcgccgccg ctccaccacc
     3781 tgttgggcgg cacgatcagc aggatgagga agatcgccgc gaagcaggcg aagatggtga
     3841 gcgcggtcat cgcctgcggg taggcgttct cgatgagctc cttcgcgccg gcgccggggt
     3901 ggtccgccag ataggcgcgt ttcgcgggga cggcccagag caggtacacc cccgtcccga
     3961 tcgccgtcat gaagaccacg gtgggcagca cgaagcgcgc caggagcttg aacagcccca
     4021 ccttgggcgt cggcccgggc tgcgcccaga gcgcgaaggc gatcgccggc accgtggcgc
     4081 cgaagaacga gagcagtgcc gactgccgcg gcgcgaaggg gaaccctccg atccccatga
     4141 cgatggcgat catcgccgcc ttgaagaaga tgcgcaccat gaagatgcgc aggatgtcgt
     4201 tcatgccgtt gatgatccgc tgcccctcgc ggaaggcgaa ggggagcggc gcgaaggtgt
     4261 ccttcatcag gatgagatcg ccgacgctgc gcgcggcctg gctgccgccc tgcatggcga
     4321 cggcgacgtt ggcctgcttg agcgcgatga cgtcgttgac gccgtcgccg atcatcgcga
     4381 cgtagcgccc gcggtcgcgc agggcctcca cgaggtcctg cttctgctcg ggcgtcacgc
     4441 ggccgaagat accggcctcg tcggccttct cggcgaagtc ctccggcgag agctcggcga
     4501 gctcggcccc ggagacggcg acgagatcgc gcgggggcgt gtccgctggg gcggcctctg
     4561 gggccgtggg ctcggctccg gtcgtctcct cctcgacccg cctcgtgacc atgtcgcagt
     4621 aggagtcgag acccacacgc cgtcgcagcg actggactcc cgcttgcgtg gcgagcgcga
     4681 ccaccgtctt cgggttgtct cccgagatga tcttgacgtc gatgccggcg tccgagaact
     4741 cctcgagggt gccgcggacg ccgggccgca gctcatcgct gaagcagatg agggcgatcc
     4801 cctcgaggcc ggggggcagg acgggcgggg cgtcggggtc gtcgctgaac gcgacggggt
     4861 cctgccgccc ggcgaagagc acgacgcgca ggccgcgcgc ggcccacgcg tcggtctgtg
     4921 cgcgccaggc gtcgccggcg ccctcgccga gcgccgggga cagcacctcg ggtgcgccga
     4981 gcacgtaggt cccgcgcgcc tccccgtcgg cgaaggcgag gccgctccac ttgcgctcgg
     5041 acgagaagaa ggcctcgtgc gcggccggca cgcgctccgc cgggagcgcg gtgcggagcg
     5101 cgtcgatggt ccggttggcg tcggtcgtgc tggcggcgaa ggcgccgagc aggcgcgcgg
     5161 cgcgcgcgtc gtcggcgccc agtcccgcca gttcctcgaa cgtcaccacg cccgacgtca
     5221 gcgtccccgt cttgtcggtg cagagcacgt cgacgttgct gaggctctcg acggcgttga
     5281 actgctgcac gagcacgtcc ttgccgagca gccgcaccgc cccgagcgcg taggtgaggg
     5341 cgatgctgag caccagcccg ttggggatga gcgccaggat gacggtggac atgcgcacgc
     5401 tctccacgaa cggcacgccg ccggcgacgt tgcggatcca caccagcgcc tcgaagacga
     5461 tgaccacgat gagcaggatg cgcacgatct gcaccacctg ccgctgcagc ggcgtgagct
     5521 cccgcttgaa gaccttcgcc ttggcggtga gctggttggc gaagctgtcc atgccgacgc
     5581 gggcggcctc gtagtagccg ccgcccgtca tgcagaagct gcccgacatg agctcgtcgc
     5641 cggggtgctt ggtgacgagg tccgactcac cggtgagcag gctctcgtcg acctccatgc
     5701 ggccctggcc gatcatcggc ccgtccacga ccacctggtc gccgggctcg agccgcagga
     5761 cgtccccgag caccacctgc gacgggtcga tctccacgag ccgcccgtcg cgcagcacgt
     5821 ccgccttggg ccgcaggagg atcgccatct tgtccatgac gcgtttggcg cggatctcct
     5881 ggaagaggct gatgacggtg ttggcgccga tcacgaacac caccgcgaac gcgtccagcg
     5941 gcttgccgag gatcaggagc aggatgccga ggaagtagaa gaggttgttg atgaagttga
     6001 agacgttgtc gcggacgatg cggccgtagg tgcggccggc cttgatctcg acgtcgttgc
     6061 cctggccggc cgcgcgccgg cggtcggcct cagcggtgct gaggccggag ggtgtcgcgc
     6121 gcaggtcgtc gcgggaggtc gccatggtca ccgcacgcta tcagcgcggc cgcgggcggt
     6181 ccaacggagc ggggagttgc gccgctgcgc gaccgtctct attgtggtgt gaa
//
LOCUS       DASUMX010000276         7489 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_390313, whole genome shotgun sequence.
ACCESSION   DASUMX010000276 DASUMX010000000
VERSION     DASUMX010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 7489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7489
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390313"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            283..1614
                     /gene="mtbB"
                     /locus_tag="VFZ86_13130"
                     /pseudo
     CDS             283..1614
                     /gene="mtbB"
                     /locus_tag="VFZ86_13130"
                     /inference="COORDINATES: protein motif:HMM:NF021053.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="[dimethylamine--corrinoid protein]
                     Co-methyltransferase"
     gene            complement(1697..2623)
                     /locus_tag="VFZ86_13135"
     CDS             complement(1697..2623)
                     /locus_tag="VFZ86_13135"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016483027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HEX5643261.1"
                     /translation="MADRVKEILSWYRSENPGTLTNLYRMLDHGTLAGTGKMVILPVD
                     QGFEHGPARSFAPNPPGYDPRYHFQLALDAGTNAYAAPLGFLEAGAAEYAGRVPLILK
                     ANNSDSLYGGSDPIPAVTASVQDALRLGAVAIGFTIYPASAARNDMYEELRELTLEAK
                     EAGLVVVVWSYPRGSGISKQGETAVDVCAYAAQIAAQLGAHIIKVKPPTEHIELDAAR
                     KVYEAQGIRVGTLAERVEHVIQSAFDGRRIVIFSGGEAKGVDAVLEENRQTALGGGFG
                     TIMGRNSFQRPHDEAVKLLRDVMEIHRTTWPS"
     gene            2691..3524
                     /locus_tag="VFZ86_13140"
     CDS             2691..3524
                     /locus_tag="VFZ86_13140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010469459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5643262.1"
                     /translation="MSARTATPTLSVTFAGHATVLLEMDGVRLLTDPLLRKRIGVLIR
                     RSPVPPPAVRRDLDAVLISHAHLDHLDVPSLRLVDRRTPVVAPRGVGPLLRRLGFVPL
                     EMEAGDEVEVGPAGAGGSPGRARVRVRAVPADHAGTRYPLAADSPALGYVVEGGLSAY
                     FAGDTGLYDGMRDLAPIDVAVLPVGGWGPRLPSDHLNPLSAAKALELLRPAACVPVHW
                     GTIYPPWLPPEFNAKWSEWPHAFARYAAHLAPEVDVRVLQPGETTVFVRAARGGEAAR
                     E"
     gene            3521..4399
                     /locus_tag="VFZ86_13145"
     CDS             3521..4399
                     /locus_tag="VFZ86_13145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007918775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEX5643263.1"
                     /translation="MSAAELRIALLQLAGSGYDREASLERGLVACRRAAAAGADVALF
                     PELWSTGYAFCDGDLERWRAQAIAGDDPWLVAHADLAAESRMAIGVTYLERWAEGPRN
                     TVTLFDASGREALRYAKVHICSFDLPEQALAPGTGFPVARLETRAGAVAVGALICFDR
                     EFPEAARVLALGGAELILVPNACELEANRLGQVRARAFENSVAVAVANYAAPQENGHS
                     TVVDPIAFDAEGRSRDTRVFEAGEAEEVCVVRLDLGALRAWRRREVWGSECRRPETYG
                     ALVEDDGGLPGGSAEL"
     gene            complement(4439..5404)
                     /locus_tag="VFZ86_13150"
     CDS             complement(4439..5404)
                     /locus_tag="VFZ86_13150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643264.1"
                     /translation="MLLGAALLPSAAGAAEGLEWQHALWTAAQLRAERPSDPVVVLLG
                     GSAAREAVVNDVSWAADVQRLGGGPVATYNLSSRLRTFEQDVALVKALPEIPLIVFIG
                     INAGRFASPFTTTTETTPPAAKPSWVRHHYRSAKIWTPERKQERVRFWLDHRQEVFDE
                     RYSDHLADLDRLIGTSVRRGYTTVVLALPRNLVAMDHALDESIDRYLRDGRRLARKHD
                     AGFVDFVPELRLADDDFYDLDHLVGSGRETYQARLALETVRLLGSVPAEAAPSPTASP
                     AGAADTGLTTRVGGAKTLLPLASAFVLLGVVLATLRRRAVVRRRR"
     gene            complement(5510..6790)
                     /locus_tag="VFZ86_13155"
     CDS             complement(5510..6790)
                     /locus_tag="VFZ86_13155"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HEX5643265.1"
                     /translation="MAYAEDFERALQVGRPPNITSLFPNSKALIVSGKVVDRAMRAKG
                     KAIAMAANGRNSFVIRGALRAAQRANAALIIEIAKSEGGQKAYCAVNYWNMARVVDAL
                     CNEMGITIPVAVHADHYGMKNEKDLAQALVEIPTMFEAGITSIAIDASHQPNDQNLLA
                     NLAVNSKIPAWAGLETEIGEIKGSTGLSTPAEALFLIQGLNAHGIAADWIALNNGTTH
                     GIEASDAGIQVGLTAEVHKVLEPYNVSGAQHGTSGNSSDRLREIAAQTHTTKANVATA
                     LQMISWGLEVNDYGNAQLDADGGFIKVEGEGVTEEMWAEVVAYADERGWKKGDYKNLN
                     LPFESKFLAQPAEIRERMARRVEDFAYKMMTEVLGGGDTAPLAVEAILAAGSYDLGPK
                     AQRIEDPADWTDEKIVERAKSLDSDKGAAGDFDD"
     gene            complement(6952..7245)
                     /locus_tag="VFZ86_13160"
     CDS             complement(6952..7245)
                     /locus_tag="VFZ86_13160"
                     /inference="COORDINATES: protein motif:HMM:NF020317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HEX5643266.1"
                     /translation="MPQYVILSNLTAKGLADLKGGPARYQSVVKAIEAAGGKVLSFHA
                     TMGAYDYVMVAEGPSDEFAALMSMSTAAQGYVTTQTLRAFTPEEFAGLVGKLP"
BASE COUNT         1004 a         2576 c         2771 g         1138 t
ORIGIN      
        1 cgtcgggatg cgctgcgcca tagatcctcc cgtcgtcccc cgaccgcggg gcgatcctcg
       61 ttagttagtc gtataactaa ccgaaagctc tctggacgat atcggcgcgg tcggccgggt
      121 gtcaagacgg cgctccgggc gtgtggtggc gctaggtgcg ccccgcgagt gggaccaccc
      181 attgcgccgg gggacgggcg tgcgtagcat ctgggcaagg gcaggacgag gcggggagag
      241 ggagcgccgg cgacggcagg gggcggtgtg gaatctccga tcgtgacgcg tcagggggac
      301 ggctcagccg tcgcgctgac gcgcagtgag ctcagggccg agctggagga gggcacgcgc
      361 gcggccgcct cacgggcgcg cgctccggag ctgcgcgcgg acgagctcga tcacctcctg
      421 gacatcttca ccagccgcgc gcgcttctcg gccgtcgaca tcggcgacga ggtcgtgctc
      481 agcttcgacg gcagcggcac gccgcagcag gggagccgga tcgacgcgct ggtgcagtac
      541 gagcagtgtc tcggagcgga cacctgcgag ctctaccaca tcgactactc ctacaaggcg
      601 gtcaagacga tcgccggcgc cgagcagcag gcgatgaagg aggcgcagga gcgcgtcacc
      661 atcccggtgc actacggcgc ccagccggac ctcggcaggt actcgcggcc ggacggcccg
      721 tgcggcaact ggtcggagct cctgccggcg ctgcgcatcg acgaggcgcg ggcggcgcag
      781 gaggaggcgg tcgagctcgc cgtggaggac atggtccacg tcgccggcgc gatggccgag
      841 gccggcgccg acggaatcaa cttcgacacc gccggcgccg ccggcgacgc cgacctcctg
      901 gcgaccctgc tcgccgtgga acgcctgcgc gcgaagtacc cagccatggg catcatggtc
      961 ggcatggccg gggaggtcgt gctcggcacg cacggcgagc tcgagtatcg cggcacgcgg
     1021 ctggccgggc tcaagccgga ggggcagtgc cgcgtcgtcc aggaggccgg cgccacgatc
     1081 ttcgggccgg ccgtcaacgt gaacaccagc cgcaccgtgg cctggaacgt ggcccgtgcg
     1141 ctcacgttca tgaagccgtg catggacgcc gcccgcatcc cggtccacgc caacgtgggc
     1201 atgggcgtcg gcggcgtgcc catgttcgcg ttcagccccg tcgacgcggt ctcgcggtgc
     1261 tcgcgcgcct gcgtcgacat cctcaaggtc gacggcttgt aggtggggtc gggagatacc
     1321 ttcggcatgc cggtcgcgca cgccgtcgcc tcggggatga acgggttgcg cgccgccgga
     1381 gacctggtgg cgcgcctcga gatggcgaag ggcatgcgac tcaaggaagc caagcggacc
     1441 gtcgccgacg cgctcggcgt cggcgtcgcc gacctcgccg acccgctggc gatgcacgag
     1501 gtccgcggcg ggctgcgact cggccgcctc ttcgaggccg agaccacgta cccgcacgac
     1561 ccgagcccgc aggaggccaa ggcgaacatc cgccgcgtgc tgggcctcgg ctgactcgcc
     1621 ggtcgccccg gtctccgcgc ggcggcctct caggctctcg gactcgcggt ctctccggcc
     1681 ccggcctcgc ggcctctcag ctcggccaag tggtgcggtg gatctccatg acgtcccgga
     1741 gcagcttcac ggcctcgtcg tgggggcgct ggaacgagtt gcggcccatg atggtgccga
     1801 acccgccgcc gagcgccgtc tgccggttct cctccaggac ggcgtcgacg cccttggcct
     1861 caccgccgga gaagatgacg atgcggcggc cgtcgaacgc gctctggatc acgtgctcga
     1921 cgcgctcggc cagcgtgccg acccggatgc cctgcgcctc gtagaccttg cgcgcggcgt
     1981 cgagctcgat gtgctccgtc ggcggcttga ccttgatgat gtgggcgccg agctgggcgg
     2041 cgatctgcgc ggcgtaggcg cacacatcga cggcggtctc gccctgcttg ctgatgccgg
     2101 acccgcgggg gtagctccac acgaccacca cgaggccggc ctccttggcc tcgagcgtga
     2161 gctcgcgcag ctcctcgtac atgtcgttgc gggcggccga ggccgggtag atggtgaagc
     2221 cgatggccac cgcgccgagg cgcagggcgt cctgtaccga ggccgtgacc gccgggatgg
     2281 ggtcggaccc gccgtagagc gaatccgagt tgttggcctt gaggatcagc ggcacgcggc
     2341 cggcgtactc ggccgcgccc gcctcgagga agccgagggg cgctgcatag gcgttcgtgc
     2401 cggcgtcgag cgcgagctgg aagtgatagc gggggtcgta gccgggggga ttcggggcga
     2461 agctgcgcgc cggcccgtgc tcgaagccct ggtcgacggg gaggatgacc atcttgcccg
     2521 tgccggcgag cgtcccgtgg tccagcatgc ggtagaggtt ggtgagcgtg ccggggttct
     2581 cggacctgta ccagctgagg atctccttga ctctgtctgc catgcttctc gcctccgtcg
     2641 gtggtcgcgt gcgtagatgg tcagtacccc gtctcgggta gagtgaggcc atgagcgcgc
     2701 ggaccgcgac ccccacactc tcggtgacgt tcgcggggca cgccaccgtg ctcctcgaga
     2761 tggacggagt ccggctgctc acggacccgt tgctgcgcaa gcgcatcggc gtcctcatcc
     2821 ggcgcagccc cgtgccgccg ccggcggtgc gtcgtgacct ggacgccgtg ctcatctcgc
     2881 acgcgcacct cgaccacctg gacgtgccgt cgctccggct cgtcgaccgg aggacgccgg
     2941 tggtcgcgcc gcgcggcgtc ggcccgctgc tgcggcggct cggcttcgtg ccgctggaga
     3001 tggaggcggg ggacgaggtg gaggtcggcc cggccggcgc cggcgggtcg ccgggccgtg
     3061 cgcgcgtacg cgtgagggcc gtccccgccg accacgccgg cacgcgttat ccgctggccg
     3121 cggattcgcc cgccctgggg tacgtcgtcg agggcgggct cagcgcctat ttcgccggcg
     3181 acaccggcct ctacgacggc atgcgcgacc tggcgcccat cgacgtcgcc gtgctgcccg
     3241 tgggcgggtg gggcccgcgc ctgcccagcg accacctcaa cccgctctcg gcggccaagg
     3301 cgctcgagtt gctgcgcccg gccgcctgcg tcccggtgca ctggggcacg atctacccgc
     3361 cgtggctccc gccggagttc aacgccaagt ggagcgagtg gccgcacgcg ttcgctcgct
     3421 acgcggcgca cctcgcgccg gaggtcgacg tgcgcgtcct gcagccgggc gagacgacgg
     3481 tgttcgtccg cgcggcgcgc gggggggagg cggcgcggga gtgagcgcgg ccgagctccg
     3541 catcgcgctg ctgcagctcg ccggctcggg ctacgaccgc gaggcgagcc tcgagcgtgg
     3601 tctcgtcgcc tgccgccgcg ccgccgcggc cggcgccgac gtcgcgctgt tcccggagct
     3661 gtggagcacc ggctacgcct tctgcgacgg cgacctggag cgctggcggg cgcaggcgat
     3721 cgccggcgac gacccttggc tcgtcgccca cgcggacctc gccgcggagt cgcgcatggc
     3781 gatcggcgtg acgtacctcg agcggtgggc tgaaggaccg cgcaacaccg tcacgctgtt
     3841 cgacgcgagc ggccgcgagg cgctcaggta cgccaaggtg cacatctgca gcttcgacct
     3901 gccggagcag gcgctggcgc cgggcacggg attcccggtc gcccggctgg agacgcgcgc
     3961 cggcgccgtg gcggtgggcg cgctgatctg cttcgaccgc gagttcccgg aggcggcgcg
     4021 cgtgctcgcc ctgggcggcg ccgagctcat cctcgtgccc aacgcctgcg agctcgaggc
     4081 gaaccgcctc ggccaggtgc gcgcgcgggc gttcgagaac tctgtcgcgg tcgccgtggc
     4141 caactacgcg gcgccgcagg agaacggcca ctcgacggtg gtcgacccca tcgccttcga
     4201 cgctgagggg cgcagccgcg acacgcgcgt cttcgaggcg ggggaggcgg aggaggtctg
     4261 cgtcgtccgc ctcgacctcg gagcgctgcg cgcctggcgc cgccgggagg tgtggggcag
     4321 cgagtgccgg cggccggaga cctacggcgc gctggtcgag gacgacggcg ggctccccgg
     4381 cgggtcagcg gagctctgag cccgtcgggg tgcgtcatgc gggccgcgcc ggcctccgtc
     4441 acctcctgcg ccggaccacg gcgcggcgac gcaacgtcgc gagcaccacg ccgagcagga
     4501 cgaacgcgga ggccagcggc agcagggtct tcgcgccgcc gacccgggtc gtcaagcctg
     4561 tgtccgcggc cccggcgggt gacgccgtgg gggagggcgc ggcctccgcc gggacggacc
     4621 cgagcaggcg cacggtctcg agcgcgagcc gcgcctgata cgtctcccgt ccggagccca
     4681 cgaggtggtc cagatcgtag aagtcgtcgt cggccaggcg cagctccggc acgaagtcga
     4741 cgaaaccggc gtcgtgcttc ctcgcgaggc gccggccgtc cctcaggtaa cggtcgatgg
     4801 attcgtcgag agcgtggtcc atggcgacga ggttgcgcgg cagagcaagg accacggtcg
     4861 tgtatccgcg cctcacggac gtgccgatga gccggtccag gtcggcgagg tgatcggagt
     4921 agcgctcgtc gaacacctcc tggcgatggt cgagccagaa cctgacgcgc tcctgcttgc
     4981 gctccggcgt ccagatcttc gcgctccggt agtggtggcg gacccacgag ggcttggctg
     5041 ccggcggagt ggtctcggtg gtggtggtga acggactggc gaaccggccg gcgttgatcc
     5101 cgatgaagac gatgagaggg atctccggca gcgccttgac cagggcgaca tcctgctcga
     5161 acgtccgcag gcgggagctg agattgtagg tcgccaccgg cccgcccccg agcctctgca
     5221 cgtcggcggc ccagctgaca tcgttgacca cggcctcgcg ggcggcggag ccacccagca
     5281 ggacgaccac gggatccgat ggccgttcgg cccgcagctg cgcggcggtc cacagcgcgt
     5341 gctgccactc gagtccctcg gctgcacctg ccgcggaggg gaggagggcg gcgccgagca
     5401 gcacaccggc cagacacgtg acgagggccg cgagccgaag gccacgcgcg ccgcggcgcg
     5461 cgtggcctcg gccctcggct cgccgaagct tgaccccgag gacggtcact cagtcgtcga
     5521 agtcgcccgc ggcgcccttg tcgctgtcca gcgacttcgc cctctcgacg atcttctcgt
     5581 cggtccagtc cgccgggtcc tcgatccgct gggccttggg gccgaggtcg taggagccgg
     5641 cggcgaggat cgcctcgacg gcgagcggcg cggtgtcgcc gccgcccagc acctcggtca
     5701 tcatcttgta ggcgaagtcc tccacgcgcc tggccatccg ctcgcggatc tccgccggct
     5761 gcgccaggaa cttgctctcg aacggcaggt tgaggttctt gtagtccccc ttcttccagc
     5821 cgcgctcgtc ggcgtaggcg accacctcgg cccacatctc ctcggtcacg ccctcgccct
     5881 cgaccttgat gaagccaccg tcggcgtcga gttgcgcgtt gccgtagtcg ttgacctcga
     5941 ggccccagct gatcatctgc agggcggtgg ccacgttggc cttggtggtg tgcgtctggg
     6001 ccgcgatctc gcgcaggcgg tccgagctgt tgcccgacgt gccgtgctgg gcgccgctca
     6061 cgttgtacgg ctcgaggacc ttgtggacct cagcagtgag cccgacctgg atgccggcat
     6121 ccgacgcctc gatgccgtgg gtggtgccgt tgttcagggc gatccagtcg gcggcgatgc
     6181 cgtgcgcgtt gagcccctgg atgaggaaca gcgcctcggc tggggtggag aggcccgtgg
     6241 agcccttgat ctcgccgatc tcggtctcga gccccgccca cgccgggatc ttcgagttga
     6301 cggcgaggtt ggccagcagg ttctggtcgt tgggctggtg ggaggcgtcg atggcgatgg
     6361 acgtgatgcc cgcctcgaac atcgtgggga tctcgaccag ggcctgggcg aggtccttct
     6421 cgttcttcat cccgtagtgg tcggcgtgca ccgcgaccgg gatggtgatg cccatctcgt
     6481 tgcagagggc gtcgacgacg cgcgccatgt tccagtagtt cacggcgcag taggccttct
     6541 gcccgccctc ggacttggcg atctcgatga tgagcgcggc gttggcccgc tgcgcggccc
     6601 gcagcgcgcc gcggatgacg aaggagttgc gcccgttggc ggccatggcg atggccttgc
     6661 ccttggcgcg catggcgcgg tcgacgacct tgccgctgac gatcagcgcc ttggagttcg
     6721 ggaacaagga cgtgatgttg ggaggacggc ccacctggag ggctctctcg aaatcctctg
     6781 cgtacgccat ggaaggtctc cttcaggggg ttgtgggcgc tggacgcgcc gtcgtttcgt
     6841 cacggattct accgaatctg cacaccatta cacacatggc gacgcgagga gagggcgggc
     6901 ccgcaggccc gccgactcct cgcgcgtctg cgccccgcac gcacccggcc gtcagggcag
     6961 cttgccgacg aggccggcga actcctcggg cgtgaaggcg cggagtgtct gcgtggtgac
     7021 gtaaccctga gcggcggtcg acatcgacat gagggccgcg aactcatcgc tcgggccctc
     7081 ggccaccatc acgtagtcgt aggcgcccat cgtggcatgg aagctgagca ccttgccgcc
     7141 ggcggcctcg atggccttga cgacagactg gtaccgtgcc ggcccgccct tgaggtcggc
     7201 tagtcctttg gcggtcaggt tggagagtat gacgtactgt ggcatggcgc acctcccgtg
     7261 ttccggcgcc gcattctcgt ctgcggcatc cgaacatggt tgtaacccct cagccgccca
     7321 atagtcaccg ttgggcggtg cgcagccgac gacgagggtc ccagggatgg caacggggat
     7381 gccaccacct ctgggtctct gcgggaatgt gtgtaccagg ccgaatcaca gaggaggtgc
     7441 ttccatggga ggcacggcag acgagagcaa gggtcgtctc aagaaggcc
//
LOCUS       DASUMX010000277         6417 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_390415, whole genome shotgun sequence.
ACCESSION   DASUMX010000277 DASUMX010000000
VERSION     DASUMX010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 6417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6417
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390415"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1895)
                     /locus_tag="VFZ86_13165"
     CDS             complement(<1..1895)
                     /locus_tag="VFZ86_13165"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEX5643267.1"
                     /translation="MTKRLLVVVAVAVAVLVLAVPAMAFNGFREEYTTSDVCAGCHSG
                     TAGEWAETKHGENEAYEEVKDRAVNGSVCQGCHTSNFDPAKAIPTPTATTTAVAPAPA
                     ATNVAWGVDQSEVGMPQATGEAPWSENMIGCSSCHYNASTAHSVPLGEMANSDICGAC
                     HSRYSYTVDTFSVTPIPYVRIDGAGSPIPNPSPTSLIQPQMALGYPMLGSPAPAPATG
                     WDPAAPLSDYLNIPAAGWTPMPDPSATSAGYGRLSTFWNVDGVEQKWQQVTHELASGQ
                     YPEWANEGHADALTGLTSQPFWGFLPEETKQECLECHSTDYVLLEEAGEDPTSADAKY
                     GVTCVGCHAPHEAGTIQGAWSEHANAQLRDDASLEGEGSDLCTKCHNGEIPEGTTASP
                     GTEIHHPMKEMIDGYGAIDVSSFPSVHKGKCIECHMPPTYWQRGSVQNGANHTFDIIE
                     PKDAVEASPVPVTTAVATATATPVPGGTPVLTVTSRVTWDSMPYSACSTCHNGNTKPS
                     PRAIATATATPNPTASPLRVNVTIDQMADGAPIYASSTNEWSPGGDKALWLQETIDQR
                     QAWTKAKIDAIWAVLDAVAVDNGYADAGAARDALVEQPSNTWTTTVRAFLSSFTNVEF
                     VESEGSFG"
     gene            2454..4355
                     /gene="dnaK"
                     /locus_tag="VFZ86_13170"
     CDS             2454..4355
                     /gene="dnaK"
                     /locus_tag="VFZ86_13170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEX5643268.1"
                     /translation="MAKVIGIDLGTTNSAMAVLEGGEPTVIPNSEGGRTTPSVVAFSK
                     SGERLVGSVARRQAVTNPENTVFSIKRFMGRKYGDVSEEMKIVPYHVVKASNGDASVD
                     IGGKTYSPPEISAMILQKLKRDAEEYLGEKVTEAVVTVPAYFNDSQRQATKDAGKIAG
                     LDVKRIINEPTAAALAYGLDKERDQTILVFDLGGGTFDVSILDLGEGVFEVRSTNGDT
                     HLGGDNFDKAVVDWMVAEFKQSQGIDLSVDKMALQRLYEAAEKAKIELSTTMSSAVNL
                     PFITADENGPKHLDLTLTRAKFDELTHDLVERVVAPVNAAIKDAGLSKGEIHDVILVG
                     GMTRVPAVQEKVKELIGKDPHKGVNPDEVVAVGAAIQGGVLAGEVKDVLLLDVTPLSL
                     GIETKGGVFTRLIDRNTTIPTKKSEIFSTADDNQTSVEIHVLQGERDMAAYNKTLGKF
                     QLVGIPLAPRGMPQIEVTFDIDANGIVHVAAKDLGTGNEQKITITASSGLSEQDIEQM
                     MKDADAHADEDKKAREMADVKNNAENLLYSTEKSLRDMGDRVDASTKAEIESAAGELK
                     TALEGDDMDAIQAKTDALMQASHKLAEAVYQQAQQEQAASSDGSGPAAEDENVEEADY
                     EVIDEDESK"
     gene            4434..4901
                     /locus_tag="VFZ86_13175"
     CDS             4434..4901
                     /locus_tag="VFZ86_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX5643269.1"
                     /translation="MAIIRWDPFREMTQVQNQFNRLVDQVWGGRQESWLPAVDVFDRK
                     DAVVLKAELAGMDPDDIEIEVEDNVLTIKGERKFEEEVEDERYYRVERRFGSFQRSLA
                     LPQGVKANEIQAGYDDGILTVTVPKAEEEQPQRIEVKAKKTVEASKSEGGATS"
     gene            5009..5599
                     /locus_tag="VFZ86_13180"
     CDS             5009..5599
                     /locus_tag="VFZ86_13180"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEX5643270.1"
                     /translation="MTDRKHTTKDQKRAKAAEAEAKPAEAPDETEATAAAEEPAPADE
                     AAESAELAAVARERDEYLDHLRRLKAEFDNYRKRVQRDNEELRLRAAETVVESLLPVM
                     DNLSRALEAGDKHGEGQLLAGLGLVGDQLRGTLAGHGLEEIAVEPGTSFDPEYHEAIM
                     AQASAEHPEGTVTQVLERGYLLHGKLLRPAKVIVAS"
     gene            5609..>6417
                     /locus_tag="VFZ86_13185"
     CDS             5609..>6417
                     /locus_tag="VFZ86_13185"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEX5643271.1"
                     /translation="MSDHYQTLGVSKNASQDEIKKAFRKLARQHHPDKNPGNTAAEEK
                     FKEVSTAYETLSDPEKRKQYDELSRLGAFGAGPGGGGFRPGAGGYQGFDPRTFNQGNV
                     QFDVGDLGDILGNLFGGAAGGAGRGRRRAAERGDDLQANVTVSFEDALNGVTVRVPVD
                     KADRCETCHGSGAKPGTTPKVCPECQGRGMVARNQGPFAMSEPCPRCHGQGTIVESPC
                     ATCHGTGVQNKTRRYNVKIPAGVKDGSKIRIKGRGEAGLRGGPAGDLYVRVR"
BASE COUNT         1164 a         2104 c         2244 g          905 t
ORIGIN      
        1 ccgaagctgc cctcgctctc cacgaactcg acgttcgtga agctgctgag gaaggcgcgc
       61 acggtcgtgg tccaggtgtt cgaaggctgt tcgacgagag catcgcgggc agcccccgca
      121 tcggcgtagc cgttgtcgac ggctactgcg tcgagcaccg cccagatcgc gtcgatcttg
      181 gccttggtcc aggcctgacg ctggtcgatg gtctcctgca gccagagagc cttgtcgccg
      241 ccaggcgacc actcattggt gctggaggcg tagatcggcg cgccgtcagc catctggtcg
      301 atcgtgacgt tgaccctcaa cgggctggcc gtcggattcg gggtcgcggt cgccgttgcg
      361 atggccctcg gcgacggttt ggtgttcccg ttgtggcacg tgctgcaggc cgagtacggc
      421 atgctgtccc acgtcaccct gctggtgacg gtgaggacgg gagtgccgcc cgggaccggt
      481 gttgcggtcg cggtggccac ggccgtggtg accggcaccg gcgaagcttc gacagcatcc
      541 ttgggctcga tgatgtcgaa cgtgtggttg gcgccgttct gcacgctgcc gcgctgccag
      601 tacgtcggcg gcatgtggca ctcgatgcac ttgcccttgt ggacgctcgg gaacgaagac
      661 acatcgatgg cgccgtagcc gtcgatcatc tccttcatcg gatgatggat ctcggtaccc
      721 ggcgacgccg tcgtgccctc ggggatctcc ccgttgtggc acttggtgca caggtcgctg
      781 ccctcgccct cgagcgacgc gtcgtcgcgg agctgggcat tggcgtgctc gctccaggcg
      841 ccctggatcg tgccggcttc atgcggtgca tggcagccca cgcaggtgac gccgtacttg
      901 gcatcggcgc tggtggggtc ctcgccggcc tcctcgagga ggacgtagtc ggtcgagtgg
      961 cactcgaggc actcctgctt cgtctcttcg ggcaggaagc cccagaaggg ctggctggtg
     1021 aggcccgtga gggcatcggc gtggccttcg ttggcccact ccggatactg accggaggcc
     1081 agctcgtgcg tcacctgctg ccacttctgc tcgacaccgt cgacgttcca gaacgtcgag
     1141 agcctgccgt aacccgcgga ggtagctgac gggtcaggca tcggggtcca gcccgcggcc
     1201 gggatgttga ggtagtcgct cagcggcgcg gcgggatccc agccggtcgc cggagcagga
     1261 gccggtgagc cgagcatcgg gtaccccaga gccatctgcg gctggatcag ggaggtcggc
     1321 gacgggttgg ggataggact ccccgcgccg tcgatcctca cgtacgggat cggcgtcacc
     1381 gagaaggtgt cgaccgtgta cgagtagcgc gagtggcagg cgccgcagat gtcggaattg
     1441 gccatctctc cgagcgggac actgtgtgcc gtgctcgcgt tgtagtgaca cgacgagcag
     1501 ccgatcatgt tctccgacca gggggcttcg cccgtggcct gcggcatccc gacctccgac
     1561 tggtccacgc cccaggccac attggtggcc gccggagcgg gagcaacggc tgtcgtggtc
     1621 gcggtaggcg tggggatggc ctttgccgga tcgaagttgg aggtgtggca gccctgacag
     1681 accgagccgt tgaccgcgcg gtccttgacc tcttcgtagg cctcgttctc gccgtgcttg
     1741 gtctcggccc actcgccggc cgtgccggaa tggcagcccg cgcacacatc gctggtcgtg
     1801 tattcctcgc ggaagccgtt gaaggccatc gcgggcaccg ccagcacgag aactgccacg
     1861 gcgacggcta caacgacaag tagtcgcttt gtcattgaca cctccttact gggtttgacg
     1921 ggatgggaaa gcctgtccgc tctcattgat cacctcccct cccgaagcgg atcatcgatc
     1981 gaacacgcct cgcgaagccc gggcgacgcg aggcgacatt cgtgcgttga ggcgagatgg
     2041 ggaagagggc gagggcgggc gtgcgcatgt gctgcgttgc acatggtcgt ccgcgggatg
     2101 tcggtgctcc aggtccccat cgtcggctcg cagaccggcg cacccggggg ggtatgccgg
     2161 tctacccgtg gaggtataca ggatttggtg ggtgttagca aacatttagc aatgttgcgc
     2221 cgagcgcctg ccgagcctgt gcagacgctg ccgggcaccc tcgtctccac ggtcctcccc
     2281 tcccggtcca gatgacctgc ccacacacga gggaaggaaa accgttggcg gtgcggaggg
     2341 gagcatcgcc ccagtcgagg gttttgacgc ccgtcggcga gggtacaaat ttgacaacgt
     2401 atgactcaga ttttatgctt cggtccagag tagtccccca aggaggaagt tccatggcca
     2461 aagtcatcgg catcgacctc ggcacgacca acagcgcgat ggccgtgctc gaaggcggcg
     2521 agccgaccgt catcccgaac agtgagggcg gtcgcaccac gccgtccgtg gtcgcgttct
     2581 cgaagagcgg cgagcgcctg gtaggcagcg tcgcccgtcg tcaggcggtc accaaccccg
     2641 agaacaccgt gttctcgatc aagcgcttca tgggccgcaa gtacggcgac gtctccgaag
     2701 agatgaagat cgtgccgtat cacgtggtca aggcctccaa cggcgacgcc tccgtggaca
     2761 tcggcggcaa gacctactcg ccgccggaga tctccgcgat gatcctgcag aagctcaagc
     2821 gggacgccga ggagtacctg ggcgagaagg tcaccgaggc ggtcgtcacc gtgccggcct
     2881 acttcaacga ctcgcagcgc caggcgacca aggacgccgg caagatcgcc gggctcgacg
     2941 tcaagcgcat catcaacgag cccaccgcgg ccgcgctcgc ctacgggctc gacaaggagc
     3001 gcgaccagac gatcctcgtg ttcgacctgg gcggcggcac cttcgacgtc tccatcctcg
     3061 acctcgggga gggagtgttc gaggtgcgct ccaccaacgg cgacacgcac ctcggcggcg
     3121 acaacttcga caaagcggtc gtcgactgga tggtcgcgga gttcaagcag agccagggca
     3181 tcgacctgag cgtcgacaag atggcgctgc agcggctgta cgaggccgcc gagaaggcca
     3241 agatcgagct ctcgacgacg atgagctcgg ccgtcaacct gccgttcatc acggccgacg
     3301 agaacgggcc caagcacctc gacctcacgc tgacgcgggc gaagttcgac gagctcacgc
     3361 acgacctggt cgagcgcgtg gtggcgccgg tcaacgccgc catcaaggac gccggcctca
     3421 gcaagggcga gatccacgac gtgatcctcg tcggcggcat gacccgtgtg ccggcggtgc
     3481 aggagaaggt caaggagctc atcggcaagg atccgcacaa gggcgtcaac ccggacgagg
     3541 tcgtcgccgt cggcgctgcc atccagggcg gcgtgctggc cggcgaggtc aaggacgtgc
     3601 tgctgctcga cgtcacgccg ctcagcctcg gcatcgagac caagggcggc gtgttcaccc
     3661 ggctcatcga ccgcaacacc acgatcccga ccaagaagag cgagatcttc agcaccgcgg
     3721 acgacaacca gacgagcgtc gagatccacg tgctccaggg cgagcgcgac atggcggcct
     3781 acaacaagac gctgggcaag ttccagctcg tgggcatccc gctggcgccg cgcggcatgc
     3841 cgcagatcga ggtcaccttc gacatcgacg ccaacggcat cgtgcacgtg gccgccaaag
     3901 acctcggcac cggcaacgag cagaagatca ccatcaccgc ctccagcggc ctcagcgagc
     3961 aggacatcga gcagatgatg aaggacgccg acgcgcacgc cgatgaggac aagaaggcac
     4021 gcgagatggc cgacgtcaag aacaacgccg agaacctgct gtacagcacc gagaagtcgc
     4081 tgcgcgacat gggcgacaga gtcgacgcct cgaccaaggc cgagatcgag tcggcggcgg
     4141 gcgagctcaa gaccgcgctc gagggcgacg acatggacgc catccaggcg aagacggacg
     4201 cgctgatgca ggcgtcgcac aagctcgccg aggccgtcta tcagcaggcc cagcaggagc
     4261 aggcggcgag ctccgacggc tccggcccgg cggcggagga cgagaacgtc gaagaggccg
     4321 actacgaggt catcgacgag gacgagagca agtaaggacg caaggaacgg cgggcggccg
     4381 ctgacgccgg cgccgcccgc ctcaaccccc gacatccaga ggaggtgtcg accatggcga
     4441 tcatcagatg ggacccgttt cgcgagatga cccaggtcca gaaccagttc aaccggctgg
     4501 tggaccaggt ctggggcggc cgccaggaga gctggctgcc ggcggtggac gtcttcgaca
     4561 ggaaggacgc cgtcgtcctc aaggccgagc tcgccggcat ggaccccgac gacatcgaga
     4621 tcgaggtcga ggacaacgtg ctcaccatca agggcgagcg caagttcgag gaggaggtcg
     4681 aggacgagcg ctactaccgc gtcgagcgcc gcttcggcag cttccagcgg agcctcgcgc
     4741 tcccgcaggg cgtcaaggcc aacgagatcc aggccggcta cgacgacggc atcctcacgg
     4801 tgaccgtacc gaaggccgag gaggagcagc cgcagcgcat cgaggtcaag gccaagaaga
     4861 cggtcgaggc ctccaagtcg gagggcgggg ccacgagctg acctgcgcgg accggcgggg
     4921 cgggggcgca gcgccgccgc cccgccggga ctctcctgcg agatcgagcg accccagggt
     4981 cgcaccagcg acgagacaga gaacggacat gacggacagg aagcacacga cgaaggacca
     5041 gaagcgcgcc aaggccgccg aggccgaggc caagccggcc gaggctccgg acgagacaga
     5101 ggccaccgcc gccgcggaag agcctgcgcc ggcggatgag gccgccgagt ccgccgagct
     5161 cgccgcggtc gcgcgcgagc gcgacgagta cctcgaccac ctgcggcggc tcaaggccga
     5221 gttcgacaac taccgcaagc gcgtgcagcg cgacaacgaa gagctgcgcc tgcgcgccgc
     5281 cgagacggtc gtcgagtcct tgctgccggt catggacaac ctcagccgcg cgctcgaggc
     5341 cggggacaag cacggggagg gccagctcct ggccggcctc ggcctggtcg gcgaccagct
     5401 gcgcggcacg ctggccggtc acggcctcga agagatagcg gtcgaaccgg gcacgtcgtt
     5461 cgacccggag taccacgaag cgatcatggc ccaggcgtcg gccgagcatc ccgagggcac
     5521 cgtgacccag gtgctggagc gcggctacct gctgcacggc aagctgctgc ggccggccaa
     5581 ggtcatcgtc gcgagctagc aggcggagat gtcggatcac taccagacgc tgggcgtctc
     5641 caagaacgcc tcgcaggacg agatcaagaa ggcgttccgc aagctcgcgc gccagcatca
     5701 cccggacaag aacccgggca acacggcggc cgaggagaag ttcaaggagg tcagcaccgc
     5761 gtacgagacg ctgtccgatc ccgagaagcg caagcagtac gacgagctca gccggctcgg
     5821 cgcgttcgga gcggggccgg gcggcggggg cttccgtccc ggcgccggcg gttaccaggg
     5881 cttcgacccg cggacgttca accagggcaa cgtccagttc gacgtgggcg acctcggcga
     5941 catcctcggc aatctcttcg ggggcgcggc gggcggcgcc ggccgcggcc ggcgccgggc
     6001 ggccgagcgg ggcgacgacc tgcaggccaa cgtcaccgtg tcgttcgagg acgcgctgaa
     6061 cggcgtgacc gtgcgcgtcc cggtcgacaa ggccgaccgg tgcgagacct gtcacggcag
     6121 cggcgccaag ccgggcacga cgcccaaggt ctgcccggag tgccagggcc gcggcatggt
     6181 ggcgcgcaac caggggccgt tcgccatgtc cgagccgtgc ccccgctgtc acgggcaggg
     6241 cacgatcgtc gagagcccgt gcgccacgtg ccacggcacg ggcgtgcaga acaagacccg
     6301 ccggtacaac gtcaagatcc cggcgggcgt caaggacggc agcaagatcc gcatcaaggg
     6361 caggggagag gccggccttc gcggcggccc ggccggcgac ctctacgtgc gcgtgcg
//
LOCUS       DASUMX010000278        17104 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1828254, whole genome shotgun sequence.
ACCESSION   DASUMX010000278 DASUMX010000000
VERSION     DASUMX010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 17104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17104
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1828254"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            239..1609
                     /locus_tag="VFZ86_13190"
     CDS             239..1609
                     /locus_tag="VFZ86_13190"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HEX5643272.1"
                     /translation="MSEPIYAQRYRTKRRLPAGRAAAAYAGEDAQGRPVVVTIVRPPD
                     PDVFLRTVGVVRSVRHLDLAAVVDAGREGPDCYVVAEDPGGTDAATLAARGPLPVPGA
                     ALAAASAAAGLAALHERGAVHGGVDPTSVVRAADGTIKLTGAGLAGAYPPPDLRPGVA
                     PEVARYLSPEEVTGRAPSPASDVYRLGLVAYLLLTGRHAFEGADASVVAQEQLDGVVR
                     PPQLLNPEVPPALAQVVMRALDKDPGARGTAAQFQADVERVLRSAQVQETVVERRPSR
                     AWLWILLVVIAALAALAIAWALGAFGGGDDVTVPDVTGMTSQQAGATLADAGLTSGRI
                     TRVQSEEDAEGTVVAQSPAAGAEVEEGSAVDLDVAGAPAPTATPVAVPNVVGSSQAGA
                     EAQLTNAGFIVVVSQAESATVPAGTVVSQDPQAGVVAAPGSSVGIVVSTGAPASTATP
                     SASPSP"
     gene            complement(1689..3644)
                     /gene="acs"
                     /locus_tag="VFZ86_13195"
     CDS             complement(1689..3644)
                     /gene="acs"
                     /locus_tag="VFZ86_13195"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEX5643273.1"
                     /translation="MADEPKLFPPSPEFQKNAYFKSMEEYEAAYKRSVEDPEGYWAER
                     AKDALVWTKDWDTVLDWSFDPKPYIKWFEGGELNAAYNCVDRHVEAGKGDKAAIIFEG
                     DPGDSYTYTYAQLKDEVSKFANVLKKYGVKKGDRVALYLPMIAELPIVMLACARIGAI
                     HMMVFGGFSAEALKARIDNSGAKILICADKGHRGAKSTPSKTNADVAIEGETTVEKVI
                     VIKRVDGDVPMTEGRDVWYHDEMAAPDITTECECVPVDAEHPLFILYTSGSTGTPKGV
                     IHTTGGYLLFAHDTSKYVFNLHDDDVHFCTADIGWITGHSYIVYGPLALGATSLLYEG
                     LPTYPEPDRYWQVVEKHKATTIYTAPTVIRSLIGQGDEWPMKHPMESLRLMASVGEPI
                     DPNSWMWYYEKVGKGRIPLSDTWWQTETGGHMIAPLAGAMTLKPGSAGRPFFGVQTAV
                     LTDKGEPADVGEDGHLVITAPWPGMIRGTWGDEDGKRFKDVYFSMFPGYFTTGDGCAI
                     DADGDHWLKGRIDDVLNVSGHRIGTSDVEGALVSAPQVAEAAVVGYPHPIKGTGIYAY
                     VVLNDGVEPNDDLKKSLVVHVRKVVGPIATPDKMQFAATGLPKTRSGKIMRRILRKIA
                     EGEFDALGDTSTLADPAVVDDLKANKQ"
     gene            complement(4009..4083)
                     /locus_tag="VFZ86_13200"
     tRNA            complement(4009..4083)
                     /locus_tag="VFZ86_13200"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4049..4051),aa:Val,seq:cac)
     gene            4159..4611
                     /locus_tag="VFZ86_13205"
     CDS             4159..4611
                     /locus_tag="VFZ86_13205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643274.1"
                     /translation="MDVLERIKRGYRAIGPGASRDVLAMFHQEQADPPEWVVDDSTRV
                     IPSRDVVAMDLFAGALPSHWEVIGVDLRQWDYYSGKCRLVVSGRFRTRPRGTWEVVPL
                     PFIHIWNLGADDQVRGVFDYLAGIEVKRRSDVRPRRGWRVWRRPQAAG"
     gene            complement(4627..5067)
                     /locus_tag="VFZ86_13210"
     CDS             complement(4627..5067)
                     /locus_tag="VFZ86_13210"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX5643275.1"
                     /translation="MQTLHASADIDAPAEQVWRVVADFARYPEWNPFIVRAAGEQRVG
                     GRLVLTIKAPDVRAVTFRPRVLDWQPGRLIRWKGAFLVPGLFDGRHALAVESLGDGVS
                     RFTTHEEVSGILVPFLGKVMRASQKGFEQMAEAVRHRVEAQAGD"
     gene            complement(5150..5869)
                     /locus_tag="VFZ86_13215"
     CDS             complement(5150..5869)
                     /locus_tag="VFZ86_13215"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HEX5643276.1"
                     /translation="MTLLRAPIDTLIVDDLRFAVRWSARRRTVGLTVRRDGGLVVAAP
                     RRTSARRLEEVVRAKLPWVRRKLAEFEALGPPPEPRHVVAGERFPYLGHDYVLTLADD
                     PPAPVTLAGAFLEVDRALDGDARGAVTAWYEARARDYIGAAVARFAPLVGATPAKVVV
                     RDLGKRRWGVCDHRTRTVSFHWQLITQPPELVDYVVVHELAHLHEPNHGPGFWRRVAE
                     VLPDWKERRKRLSQRGDRVVF"
     gene            complement(5996..6199)
                     /locus_tag="VFZ86_13220"
     CDS             complement(5996..6199)
                     /locus_tag="VFZ86_13220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006303086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX5643277.1"
                     /translation="MAEGTVKWFNAAKGYGFISREDGDDLFVHFREIQADGFRTLDEG
                     DKVTFDVTEGQNGKMQASNVRKA"
     gene            complement(6443..7738)
                     /locus_tag="VFZ86_13225"
     CDS             complement(6443..7738)
                     /locus_tag="VFZ86_13225"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX5643278.1"
                     /translation="MSGPGTGMSRAATRVLRRHLVGPRQRWAFAPPPAGWTPPPVERT
                     SLYLHVPFCRDRCPYCPYTTVPYREGLVQGWERAALAETDLWAAAVGPAEVTSIYIGG
                     GTPTLALESVARVLERMRARFRLTGDVCIETSPSAVDEALPERLVAAGVTMVSLGVES
                     FQARHLRAVGRRYTPDQAEEALARLAGAGFAGLNADLMIALPEQTAADVVSDLDRAAQ
                     LGAGQITVYPLFTFPYTAVGAYRRRAGVGMPRLAARHEHYRAVTAWAARRGYRRVSVW
                     SFRRGDEPRYSSVTRDGYLGVGPGAGSHLPDGFVLNTFDLAAWEAALAGGAPPVALRM
                     PFSGHMAGWWWLYWRFYDTRIPLAELNTVLGGDAAKARRWLRAIESAGLAHRTDGELV
                     LTDAGCFWLHLAQNHFALAYVDALWTAGRERAWPGTVAL"
     gene            complement(7735..8616)
                     /locus_tag="VFZ86_13230"
     CDS             complement(7735..8616)
                     /locus_tag="VFZ86_13230"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX5643279.1"
                     /translation="MDGRLAWYARYAWRAAARGRVDPLFYGVALTDRCNLRCAGCRIP
                     DLGRPDISWPALDSLLRRAHARGFRELYFSGGEPMLWRDGGHTVEDGIRLARRLGFYH
                     VHLYTNGTLGLATSADLVWVSVDGLPETYRRRRGEHFATVEAAIRSRGHPPVAVLYVV
                     DSSTRDGVRPFLRWVRHSALPVLGVMFYFQTPYYGYDELFLDAAERAPVIDELLACRR
                     EGLPVLNSRAGLLALRSGRWTRRSPAAAVADVDGESVCCRAPDWCCEDCGYAACTEIV
                     EALRLRPSAVRAMGRYR"
     gene            complement(8740..10521)
                     /locus_tag="VFZ86_13235"
     CDS             complement(8740..10521)
                     /locus_tag="VFZ86_13235"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX5643280.1"
                     /translation="MSDPAEISPFDLERMDIVAVGHVDHGKSTVIGRLMADTGSLPDG
                     KLEQVKQMCKVNARPFEYAFLLDALKNEQAQGITIDTARCFFNTPQRHYIIHDAPGHV
                     EFLKNMITGASRAQAALLVIDAHEGVRENSKRHGYILSMLGIRQISVLVNKMDLLGWD
                     QAKFEAIVAEYADFLGRLGVHPTSFIPISARDGDNIVSRATGAPWYDGPTVLDQVEDF
                     KRLDDDLDRPFRMPLQDVYKFTEAQDDRRIFVGTIETGRVRPGDDVVFLPSRKHSTVQ
                     TIEALSGPKPDEAHAGQATGLTLETQVYAKPGELMVRADQAEPLVATRLRANIFWMNT
                     APLVRGRRYVLRVGAARVAAELESVLSVLDEQELESVTGAGQVERHDVAEVVLRAVRP
                     VAFDAADTLEPTSRFVIVHGHDIAGCGIALEALEDQGRGDVTGRRFRRGDVTEQERAK
                     QYGHAGKAIVFVAESGESAHDIAARVERSIFARGLHSFCLSATDLGDPKDVLDREAHL
                     GRVGEIAWAMTDAGIVLVAALGDVDRFDLDRLRRLAAPHEVFVVGVDAGAEVGADVEL
                     SAAATADDAAEEALAALTGAGVLPAKA"
     gene            complement(10518..11399)
                     /gene="cysD"
                     /locus_tag="VFZ86_13240"
     CDS             complement(10518..11399)
                     /gene="cysD"
                     /locus_tag="VFZ86_13240"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009336 - sulfate adenylyltransferase
                     complex (ATP) [Evidence IEA];
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA];
                     GO_process: GO:0070814 - hydrogen sulfide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEX5643281.1"
                     /translation="MNQLDTLEAKSVHILREAYRALGNVCMLWSIGKDSTVLLHLARK
                     AFFGHVPIPLVHIDTAYKMPEMIEYRDRLALEWRLNLVVGQNRKALEEKATFPDGNCD
                     RIQCCRNLKTMALKHTLDGEWPRLRMDHTLGRLVEDQDLSPYTGVIVGVRADEEGSRS
                     KERYFSPRDVENDWNVDDQPPELWRYFKTDFEPGTHVRVHPLLDWTELNVWEYIEREN
                     IPVTSLYFDDGSGTRCRSLGCYPCTTPVQSTATNVAEIIEELRTGKFSNIAERSGRAQ
                     DKDDGGGLESLRRGGYM"
     gene            complement(11410..12171)
                     /locus_tag="VFZ86_13245"
     CDS             complement(11410..12171)
                     /locus_tag="VFZ86_13245"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF002537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEX5643282.1"
                     /translation="MTPMPESETVPSLIVELADATAEEVLAAVAERFSGHVAFASSLG
                     AEDQVLTHMIAAAGLDIPIFTLDTGRLFPETYDLLDRTAKHYDLCIKTYFPAAAEVEK
                     MVDRDGVNLFRDSIEARHRCCEVRKILPLRRAQLELTAWICGLRSGQGATRQKVEIAE
                     WDQLADMVKINPLASWDEARVWEFIRANDVPYNPLHDQGFPSIGCAPCTRAVAEGDDW
                     RAGRWWWESAEHRECGLHDRAARIPGSAADAAAGA"
     gene            complement(12289..12924)
                     /locus_tag="VFZ86_13250"
     CDS             complement(12289..12924)
                     /locus_tag="VFZ86_13250"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF002537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEX5643283.1"
                     /translation="MTVLEPVADILERLRDLPAEAILADAAGRYAGRLAFATSLGIDD
                     QVVTHMIAEAGLGIPIFTVDTGRLFPETHDLIERTSERYGVSIAVYCPDAAELEQMVA
                     RDGINLFRHSAFKRDLCCETRRVRPLRRAQIGLDVWICARHGGAGEHETLEPAAWDAS
                     AGLVTINPLAAWDEAAVWDYARAHDVPYNPLHAQGYATIDCAPCSSPVREA"
     gene            complement(13039..14163)
                     /locus_tag="VFZ86_13255"
     CDS             complement(13039..14163)
                     /locus_tag="VFZ86_13255"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015095396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEX5643284.1"
                     /translation="MDATSQRAHRRRPAGPLLPREEMLTALRERDRSYDGLFYAAVTT
                     TGVFCRPSCPARDPKPEHVEFYATTREALVAGFRPCKRCRPLDAEAGAPEWASRLIAR
                     VEDSPSARISDADLRAEGLDPVAVRRWFRRTHGMTFQAYCRARRLGDAFAALRSGGVI
                     DEVVFDHGWGSHSGFRTAFGKLAGAPPGEAAAAPDGSLIRLTWLQTPVGPLVAGATDD
                     AVCLLEYPDRRMMESQLATLRRRFGRPLAPGDSPLFAPLRAQLDEYFAGRRTAFDLPL
                     EYPGSPFQRRVWDALRRIPYGETRSYAQLAREIGAPGSARAVGTANGANRIAIVIPCH
                     RVIAADGGLGGYGGGLWRKLRLLELEGGAASAAPAAPAER"
     gene            complement(14242..14994)
                     /locus_tag="VFZ86_13260"
     CDS             complement(14242..14994)
                     /locus_tag="VFZ86_13260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643285.1"
                     /translation="MRLRRWQVEIAATLIVAAIVVYALRWILYPSESFHDEMLRYLVD
                     DIAFLFIQVLLVSMLIDGLMQRRQREAMLDKLNMIVGAFFSECGTDVLGRIARLDREL
                     DKVREDLLARGTWTAKDYERAKQAFRAHEPVIELSGGDLAALRERLDKEKTYLLSLLG
                     NQALMEHEAFTDLLWAVTHLAEELQARVDFDHLPQADRAHLVADVKRAYTLLGVLWID
                     YLQHLQTQYPFLFSLAVRTNPLDPDANVTVAD"
     gene            complement(15234..15716)
                     /locus_tag="VFZ86_13265"
     CDS             complement(15234..15716)
                     /locus_tag="VFZ86_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643286.1"
                     /translation="MTKKIVYVDMDNVLVDFRSALPHLKKKVRKQYADHLDDVPGIFS
                     LMDPMPGALCAFTELALLFDTYILSTSPWDNPSAWSDKLCWVKTHLGEPAYKRLILTH
                     HKELHRGDFLIDDRTKNGADGFEGKHLHFGMEKFPDWPAVMAYLRPRVLRKKPWHAAP
                     "
     gene            15878..16096
                     /locus_tag="VFZ86_13270"
     CDS             15878..16096
                     /locus_tag="VFZ86_13270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008338927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEX5643287.1"
                     /translation="MARVAVEPVAIEKNGRPVAVLLSSEEYELLQRSDDFFWGQAARA
                     AEAEGFLSAEDSLRYLQDGASAEDRAAS"
     gene            16179..16343
                     /locus_tag="VFZ86_13275"
     CDS             16179..16343
                     /locus_tag="VFZ86_13275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643288.1"
                     /translation="MADPRPADAERLKGYEYSRVTVGEYRIIFDVRDGLLRVLAIGKR
                     NDDEVYRRLS"
     gene            complement(16363..16454)
                     /locus_tag="VFZ86_13280"
     tRNA            complement(16363..16454)
                     /locus_tag="VFZ86_13280"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16418..16420),aa:Ser,seq:cga)
     gene            complement(16578..>17104)
                     /locus_tag="VFZ86_13285"
     CDS             complement(16578..>17104)
                     /locus_tag="VFZ86_13285"
                     /inference="COORDINATES: protein motif:HMM:NF024585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX5643289.1"
                     /translation="DADACIDCGRCDKVCMMNVEVATADAVTSSECISCNECVNACPV
                     PGALKVTAPGGARATALVATGAVVALMVAIVGVTTLTGAFDWQMEGRAEARGAGHSGG
                     GEGQGQGGSDSPIKGWMSFADIAAMTGIPLQEFSAEWGVPAEALGLPMKDVKDQYGFS
                     PEDVRAWVEDRLAE"
BASE COUNT         2344 a         5863 c         6339 g         2558 t
ORIGIN      
        1 gccgcgtcgt gcgtccacga cgagccgccg gcccgcgacc cgcggggtga gtccgagcgc
       61 gactgcgggg cctcggcggt gagctgctcc gcgcgttggg tgtccggctc gagcttcttg
      121 tgaagtttaa cgcttgcggg cgcgcggttc accctctccg gctgcacggg ccgacgtttc
      181 ctgccctccg actcgggtaa gaccgggccg aacagaactc cggtcccaag gaggcggcat
      241 gtccgagccc atctacgcgc agcgctacag gaccaagagg cgtctgcctg cgggccgcgc
      301 cgccgcggcg tacgcgggcg aggacgcgca ggggcggccg gtggtcgtca ccatcgtccg
      361 gccgcccgac cctgacgtct tcctgcgcac cgtgggcgtg gtgagatcgg tgcgccacct
      421 cgacctcgcc gccgtcgtcg acgccggccg cgagggcccg gactgctacg tcgtcgcgga
      481 ggatcccggg gggaccgacg ccgcgaccct cgccgctcgg gggccgctcc cggtgccggg
      541 cgcggcgctc gcggcggcct ccgccgccgc cggcctcgcc gcgctccacg agcgcggcgc
      601 ggtgcacggt ggcgtcgacc ccacgtcggt cgtgcgcgcg gccgacggca ccatcaagct
      661 caccggcgcc ggcctggcgg gggcgtaccc accgccggac ctccggccgg gcgtcgcgcc
      721 cgaggtggcg cgctacctca gcccggagga agtgaccggg agggcgccgt caccggcctc
      781 ggacgtgtac cggctcggcc tcgtggccta cctgctgctc accggccgcc acgcgttcga
      841 gggcgccgac gccagtgtcg tcgcgcagga gcagctggac ggcgtggtgc ggccgccgca
      901 gctcctcaac cccgaggtgc cgccggcgct cgcgcaggtc gtcatgcgcg cgctcgacaa
      961 agacccgggc gcgcgcggca cggcagcgca gttccaggcc gacgtcgagc gcgtgctgcg
     1021 ttcggctcag gtgcaggaga ccgtggtcga gcgtcgcccg agccgggcct ggctctggat
     1081 cctgctcgtg gtcatcgccg cgctggccgc gctcgcgatc gcctgggcgc tcggcgcgtt
     1141 cgggggcggc gacgacgtca cggtcccgga cgtgacgggc atgacctcgc agcaggccgg
     1201 ggccacgctc gccgacgccg gcctcaccag cggcaggatc acccgcgtcc agagcgagga
     1261 ggacgcggag ggcacggtcg tcgcgcagtc gccggcggcg ggcgctgagg tcgaggaggg
     1321 ctcggccgtg gatctcgacg tcgccggcgc cccggcgccg acggcgacgc cggtggcggt
     1381 gccgaacgtc gtcgggagct cgcaggccgg cgccgaggcg cagctgacca acgccggctt
     1441 catagtcgtg gtcagccagg cggagagcgc gaccgtgccg gcgggcaccg tggtctcgca
     1501 ggacccacag gccggggtgg tcgccgcgcc gggcagcagc gtcggcatcg tggtctccac
     1561 gggcgccccc gcgtcgacgg cgacgccgtc ggcgtcgccc agcccctgag cgcagcgccc
     1621 gacgcgaaga gggcgggcgg cctgccggcc gcccgccctc ccgaggcgct ggtctctgga
     1681 gcgcgagact actgcttgtt ggccttgagg tcgtcgacga cggccggatc ggccagcgtc
     1741 gacgtgtcgc ccagagcgtc gaactcgccc tcggcgatct tgcgcaggat gcggcgcatg
     1801 atcttgcccg agcgggtctt cggcaggccg gtcgcggcga actgcatctt gtcgggcgtg
     1861 gcgatggggc ccacgacctt gcgcacgtgc acgacgaggg acttcttgag gtcgtcgttc
     1921 ggctcgacgc cgtcgttgag cacgacgtag gcgtaaatgc cggtgccctt gatcgggtgc
     1981 gggtagccca ccactgcggc ctcggcgacc tggggagcgc tcacgagcgc gccctcgaca
     2041 tccgaggtgc cgatgcggtg gccggagacg ttgagcacgt cgtcgatgcg gcccttgagc
     2101 cagtgatcgc cgtcggcgtc gatggcgcag ccgtcaccgg tggtgaagta gcccgggaac
     2161 atcgagaagt agacgtcctt gaagcgcttg ccgtcctcgt cgccccaggt gccgcggatc
     2221 atgcccggcc acggagcggt gatgacgagg tgcccgtcct caccgacgtc ggccggctca
     2281 cccttgtcgg tcagcacggc ggtctgcacg ccgaagaagg gacgaccggc ggagcccggc
     2341 ttgagggtca tggcgccggc cagcggggcg atcatgtggc cgccggtctc ggtctgccac
     2401 cacgtgtcgc tcagcgggat gcggcccttg cccaccttct cgtagtacca catccagctg
     2461 ttgggatcga tgggctcgcc caccgaggcc atcaggcgca gggactccat ggggtgcttc
     2521 atcggccact cgtcgccctg gccgatgagg ctgcggatga cggtcggcgc ggtgtagatc
     2581 gtggtggcct tgtgcttctc gacgacctgc cagtagcggt ccggctcggg ataggtcggc
     2641 aggccctcgt agaggaggct ggtggcgccg agggcgaggg ggccgtacac gatgtagctg
     2701 tggcccgtga tccagccgat gtcggcggtg cagaagtgga cgtcgtcgtc gtgcaggttg
     2761 aagacgtact tgctggtgtc gtgcgcgaag agcaggtagc cgccggtggt gtggatgacg
     2821 cccttggggg tgcccgtcga gccggaggtg tacaggatga agagcgggtg ctcggcgtcg
     2881 accggcacgc actcgcactc ggtcgtgatg tccggagcgg ccatctcgtc gtggtaccac
     2941 acgtcgcggc cctcggtcat cggcacgtcg ccgtcgacgc gcttgatgac gatgaccttc
     3001 tccaccgtcg tctcgccctc gatggcgacg tcggcgttgg tcttggacgg cgtgctcttg
     3061 gcgccgcggt gacccttgtc ggcgcagatg aggatcttgg cgccgctgtt gtcgatgcgc
     3121 gccttgagcg cctcggcgct gaagccgccg aacaccatca tgtggatggc gccgatgcgc
     3181 gcgcaggcga gcatgacgat cggcagctcg gcgatcatcg gcagatagag ggcgacgcgg
     3241 tcacccttct tgacgccgta cttcttgagc acgttggcga acttgctgac ctcgtctttg
     3301 agctgggcgt acgtgtacgt gtacgagtcg ccggggtcgc cctcgaagat gatggcagcc
     3361 ttgtcgccct tgccggcctc gacgtggcgg tcgacgcagt tgtaggccgc gttgagctcg
     3421 ccgccctcga accacttgat gtacggcttg gggtcgaagc tccagtcgag gaccgtgtcc
     3481 cagtccttgg tccacacgag cgcatccttg gcgcgctcgg cccagtagcc ctcagggtct
     3541 tcgaccgacc gcttgtacgc ggcctcgtac tcctccatcg acttgaagta cgcgttcttc
     3601 tggaactcgg gcgacggtgg gaaaagcttg ggttcgtcag ccaccctgtt cacctcccat
     3661 ggacctttca ctgatccgtt agctggatgg ttccttccgc gcagtgccca gggggggtcg
     3721 cgcggggggc tcgtccgtgc cggcgggcat accaggcttc cgtgcggcgg gaccagccag
     3781 cggggcgacc acagtcgtcg ctccagtgcc gcccgctggt cctggaccgt gcgggctgga
     3841 cgacggcaat gggcgccccg tgccttgggc taggccaatg ggcaacgcta ctgatgggca
     3901 gggggaaaag caagcggccg ggggccggtt catcgggttg gatgaacggc gtcgccgaag
     3961 gtggcacttg cggcggggtc ggtccaccgt gcggtcggtg tgtccgtatg gtgggcgatg
     4021 ttggatttga accaacgacc tcccgcgtgt gaggcgggcg ctctcccgct gagctaatcg
     4081 cccacggatc cgggcgcgcc tggcggcccg taggggagca gaggatacca cagcccatgc
     4141 ggcggggaaa acgagaccat ggacgtgctg gagcgcatca agcgcggcta ccgcgccatc
     4201 ggtcccgggg cgtcgcggga cgtgctcgcc atgttccacc aggagcaggc cgaccccccc
     4261 gagtgggtgg tcgacgacag cacccgcgtg atccccagcc gcgacgtcgt cgcgatggac
     4321 ctgttcgccg gcgccctgcc ctcgcactgg gaggtgatcg gggtcgatct tcgccagtgg
     4381 gactactaca gcggcaagtg ccggctcgtg gtgtcgggcc ggttccgcac gaggccgcgg
     4441 gggacgtggg aggtggtccc gctgccgttc atccacatct ggaaccttgg cgcggacgac
     4501 caggtgcgcg gagtgttcga ctacctcgcc ggcatcgagg tgaagcgcag gtccgacgtg
     4561 cggccgcgtc gcggctggcg cgtctggcgg cggccgcagg cggcggggta gcgacggtgc
     4621 cctgagtcag tcgccggctt gcgcctcgac acggtggcgc accgcttccg ccatctgctc
     4681 gaatcccttc tgggaggcgc gcatcacctt gccgaggaac ggcacgagga tgccgctgac
     4741 ctcctcgtgc gtcgtgaacc ggctgacgcc gtcgcccaga gactcgacgg cgagcgcgtg
     4801 acggccgtcg aacaggccgg ggaccaggaa cgcccccttc cagcggatga gccgtccggg
     4861 ctgccagtcc agcacgcggg gccggaacgt gacggcgcgg acgtccggcg ccttgatggt
     4921 cagcaccagg cgcccgccga cgcgctgctc gccggcggcg cggacgatga acgggttcca
     4981 ctccgggtag cgggcgaagt cggcgacgac gcgccagacc tgctcggcgg gcgcgtcgat
     5041 gtcggcggat gcgtgcagtg tctgcatggc ggcctccaga ggattctctg ccgcgagcct
     5101 acccggggag gcggctcgga gaacgccctc gaccgccggc cgcgcgccgt cagaacacga
     5161 cccggtcgcc gcgctgcgag agccgcttgc ggcgctcctt ccagtcgggc agcacctcgg
     5221 cgacacggcg ccagaacccg ggtccgtggt tcggctcgtg caggtgcgcc agctcgtgga
     5281 ccacgacgta gtccacgagc tccgggggct gcgtgatgag ctgccagtgg aagctgacgg
     5341 tacgggtccg gtggtcgcag acgccccagc ggcgcttgcc gaggtcgcgg accacgacct
     5401 tagcgggggt cgcgccgacg aggggcgcga agcgcgccac ggccgcgccg atgtagtccc
     5461 gcgcccgcgc ctcgtaccag gcggtcaccg cgccccgagc gtcgccgtcc agggcgcggt
     5521 ccacctcgag aaacgcgccg gcgagggtga cgggtgcggg cgggtcgtcc gcgagcgtga
     5581 gcacgtagtc gtgcccgaga taggggaacc gctcgccggc gacgacgtgc cggggttcgg
     5641 ggggcgggcc cagagcttcg aactcggcga gcttgcgccg cacccacggc agtttggcgc
     5701 gcacgacctc ctcgagcctg cgcgccgagg tccgtcgcgg cgccgcgacg accagcccgc
     5761 cgtcgcgcct gacggtgagg ccgacggtgc gcctgcgcgc gctccagcgc accgcgaacc
     5821 gcaggtcgtc gacgatgagc gtgtcgatgg gggcgcggag caaggtcatc ccgacggcct
     5881 ggcgcgctca ggcggggaga cccgccggcg cgctcgcgcg ctcggcgccg tacgcgacga
     5941 gggcggcccc ggaaagggac cgccctcgtc gcggcttcgg ctgttccgcg cggcgtcagg
     6001 ccttacggac gttgctcgcc tgcatcttgc cgttctggcc ttcggtgacg tcgaaggtga
     6061 ccttgtcacc ctcgtcgagg gtacggaagc catcagcctg gatctcacgg aagtggacga
     6121 acaagtcgtc gccatcttca cgggagatga agccgtaccc cttggccgcg ttgaaccact
     6181 tgacggtgcc ttctgccatt tgacgcatcc ttttccgccc ccgaagggct taaacgaagc
     6241 gacgcgaccg agtgcctgat ccgctgtgca cgccggcggg aggaacatgg tcacgccgcc
     6301 aactctaaga gcccctttgg cccgtgcgcg tcatactacc gcaccttgtc aagaattgcc
     6361 cgtcgcaggc gtggacggga cggatgacga tggccggcgg ccggggttga cgaaggcccg
     6421 cggcccgcag gtcgggccgc cgtcagagcg cgaccgtccc gggccaggca cgctcccggc
     6481 ccgcggtcca cagggcgtcg acgtaggcga gcgcgaagtg gttctgggcg aggtggagcc
     6541 agaagcatcc ggcgtcggtc aggacgagct ccccgtcggt gcggtgagcc agaccggcgg
     6601 actctatcgc ccgcagccag cggcgcgcct tggccgcgtc gccgccgagc acggtgttca
     6661 gctccgcgag agggatgcgc gtgtcgtaga accgccagta gagccaccac cagcccgcca
     6721 tgtggcccga gaacggcatg cgcagggcga cgggcggcgc gcctcccgcg agggccgcct
     6781 cccaggccgc gaggtcgaac gtgttgagga cgaacccgtc ggggaggtgc gacccggccc
     6841 cgggccccac tccgaggtag ccgtcccggg tgacggacga gtagcgcggc tcgtcgccgc
     6901 ggcggaagct ccagaccgag acgcgccggt agccgcgacg ggccgcccac gcggtgaccg
     6961 cccggtagtg ctcgtgccgc gccgccaggc ggggcatccc gacgccggcg cggcgccggt
     7021 acgcgccgac cgccgtgtac gggaacgtga acagcggata cacggtgatc tggccggcgc
     7081 cgagctgcgc cgcgcggtcg aggtcggaga ccacgtccgc ggccgtctgc tcgggcagcg
     7141 cgatcatcag gtcggcgttc aggccggcga agcccgcgcc ggcgagccgc gccagcgcct
     7201 cctcggcttg gtccggcgtg tagcggcggc ccaccgcgcg caggtggcgc gcctggaacg
     7261 actccacgcc gagcgagacc atcgtgacgc cggcggcgac cagccgctcc gggagcgcct
     7321 cgtcgacggc cgacgggctg gtctcgatgc acacgtcccc cgtgaggcgg aaccgcgcgc
     7381 gcatccgctc cagtacgcgc gccacgctct cgagcgccag cgtcggcgtt ccgccgccga
     7441 tgtagatgct cgtcacctcc gccgggccca ccgcggccgc ccagaggtcg gtctcggcga
     7501 gggcggcgcg ttcccagcct tgcaccaggc cctcccggta cggcaccgtg gtgtacgggc
     7561 agtaggggca ccggtcgcgg cagaacggca cgtgcaggta cagggaggtg cgctccaccg
     7621 gcggcggcgt ccacccggcg ggcggcggag cgaacgccca gcgctggcgc gggcccacga
     7681 ggtgccgccg gagcacgcgg gtcgcggcgc gcgacatgcc cgtgcccggg ccgctcatcg
     7741 gtagcgcccc atggcgcgga cggcgctggg ccgcaggcgc agcgcctcga cgatctcggt
     7801 gcaggcggcg tagccgcagt cctcgcagca ccagtcgggc gcgcggcagc acacggattc
     7861 gccgtcaacg tcggcgaccg cggcggccgg ggagcggcgt gtccagcggc cggagcgcag
     7921 ggcgagcagt cccgcgcgtg agttgaggac ggggagtccc tcgcgccggc aggcgagcag
     7981 ctcgtcgatc accggcgccc gttctgcggc gtccaggaac agttcgtcgt agccgtagta
     8041 gggcgtctgg aagtagaaca tgacgcccag caccggcagg gcggagtggc gcacccagcg
     8101 caggaacggc cgcacgccgt cgcgggtcga gctgtcgacg acgtagagca cggcgaccgg
     8161 cgggtgcccg cgcgagcgga tcgccgcctc gaccgtggcg aagtgctcgc cgcgccgccg
     8221 ccggtacgtc tccggcaggc cgtccacgct cacccagacg aggtcggcgg acgtggcaag
     8281 gccgagcgtc ccgttggtgt acaggtggac gtggtagaag ccgagacgcc gggcgaggcg
     8341 gatgccgtcc tcaacggtgt ggccgccgtc ccgccagagc atcggctcgc cgccgctgaa
     8401 gtagagctcg cggaagccgc gggcgtgagc gcgccgcagt agggagtcga gcgccggcca
     8461 gctgatgtcc ggccgcccga ggtcgggtat gcggcagccg gcgcagcgca ggttgcagcg
     8521 gtcggtgagg gcgacgccgt agaagagagg gtcgacccgg ccgcgcgcgg ccgcgcgcca
     8581 agcgtagcgc gcgtaccagg cgagcctgcc gtccaccgtc cccgccccct tccgggccct
     8641 tcaggacgtc ctcgatgaga gtattccctc cggcgaacgt ccggggagcg gcggcgggac
     8701 gccggcggca ggcggatcgc gccggatccc cgccgcggct caggccttgg cgggcagcac
     8761 gccggcgccg gtgagggcgg cgagagcctc ctccgcggcg tcgtcggcgg tcgccgccgc
     8821 tgagagctcg acgtcggctc cgacctccgc tccggcgtcg accccgacca cgaacacctc
     8881 gtgcggcgcc gccaggcgcc gcaggcggtc gaggtcgaag cggtccacgt ccccgagcgc
     8941 tgcgaccagc acgatcccgg cgtcggtcat ggcccaggcg atctctccga cgcggccgag
     9001 gtgcgcctcg cggtcgagca cgtccttggg gtcgccgagg tccgtggcgc tcagacagaa
     9061 ggagtgcagg ccgcgcgcga agatgctgcg ctccacgcgc gccgcgatgt cgtgtgcgga
     9121 ctcgcccgac tcggcgacga agacgatcgc cttgccggcg tggccgtact gcttcgcgcg
     9181 ctcctgctcg gtgacgtcac cgcggcggaa gcggcggccg gtgacgtcac cgcgcccctg
     9241 gtcctccaga gcttccagcg cgatgccgca cccggcgatg tcgtgcccgt gcacgatgac
     9301 gaaccgtgag gtgggctcga gcgtgtcggc ggcgtcgaac gccaccgggc gcacggcgcg
     9361 gaggacgact tctgccacgt cgtgccgctc cacctggccg gctcccgtga ctgactcgag
     9421 ttcctgttcg tcgagcacgc tgagcacgga ctcgagctcg gcggcgacgc gggctgctcc
     9481 cacgcgcagc acgtagcgcc gcccgcgcac gagcggcgcc gtgttcatcc agaagatgtt
     9541 cgcgcgcagg cgcgtcgcga cgagcggctc cgcctggtcg gcgcggacca tgagctcacc
     9601 cggcttggcg tacacctgcg tctcgagcgt gaggccggtg gcctgcccgg cgtgcgcctc
     9661 gtccggcttg gggcccgaca gcgcctcgat ggtctggacc gtcgagtgct tgcgcgacgg
     9721 caggaagacg acgtcgtcgc cggggcgcac gcgcccggtc tcgatggtgc ccacgaagat
     9781 gcgccggtcg tcctgcgcct ccgtgaactt gtagacgtcc tggaggggca tgcggaacgg
     9841 gcggtcgagg tcgtcgtcga gccgtttgaa gtcctcgacc tggtcgagca cggtcggccc
     9901 gtcgtaccag ggagccccgg tggcgcgcga gacgatgttg tcgccgtcgc gcgcgctgat
     9961 cgggatgaag ctcgtggggt gcacgccgag cctgccgagg aagtcggcgt actcggcgac
    10021 gatcgcctcg aacttggcct ggtcccaacc cagcaggtcc atcttgttga ccagcacgct
    10081 gatctggcgg atgccgagca tcgagaggat gtagccgtgc cgcttggagt tctcgcgcac
    10141 gccctcgtgg gcgtcgatga cgagcagcgc ggcctgggcg cgcgacgccc cggtgatcat
    10201 gttcttgagg aactccacgt ggcccggggc gtcgtggatg atgtagtggc gctgaggcgt
    10261 gttgaagaag cagcgcgccg tgtcgatggt gatgccctgc gcctgctcgt tcttcagggc
    10321 gtccagcagg aacgcgtact cgaaggggcg cgcgttcacc ttgcacatct gcttgacctg
    10381 ctcgagcttg ccgtcgggca gagagccggt gtcggccatg aggcggccga tgacggtgct
    10441 cttgccgtgg tccacgtggc cgacggccac gatgtccatg cgctccaggt cgaacgggga
    10501 gatctcggcc gggtcgctca catgtacccc ccgcggcgga gcgactcgag gccgccgccg
    10561 tcatccttgt cctgggcgcg gccgctgcgt tccgcgatgt tgctgaactt gccggtgcgc
    10621 agctcttcga tgatctcggc gacgttggtg gccgtggact gcaccggcgt cgtgcacggg
    10681 tagcagccca gcgaacgaca gcgcgttccg ctgccgtcgt cgaagtagag cgacgtgacc
    10741 gggatgttct cgcgctcgat gtactcccac acgttgagct cggtccagtc gagcagcggg
    10801 tgcacgcgca cgtgcgtgcc gggctcgaag tcggtcttga agtagcgcca cagctccggc
    10861 ggctggtcgt cgacgttcca gtcgttctcg acgtcacgcg gcgagaagta gcgctccttg
    10921 gagcgcgagc cctcttcgtc ggcgcgcacg ccgacgatca cacccgtgta gggagagagg
    10981 tcctggtcct ccacaagcct gcccagggtg tggtccatgc gcagccgcgg ccattcaccg
    11041 tcgagggtgt gcttgagcgc catcgtcttg aggttgcggc agcactggat gcggtcgcag
    11101 ttgccgtcgg ggaaggtagc cttctcctcg agcgccttgc ggttctggcc gacgaccagg
    11161 ttcaggcgcc actcgagcgc cagccggtcg cggtactcga tcatctccgg catcttgtag
    11221 gccgtgtcga tgtgcaccag cgggatcggc acgtggccga agaacgcctt gcgcgccagg
    11281 tgcagcagca ccgtgctgtc cttgccgatc gaccagagca tgcagacgtt gcccagcgcg
    11341 cggtaggcct cgcgcaggat gtggacgctc ttggcctcga gggtgtcgag ttggttcacc
    11401 gggcgcctct caggcgccgg cggcggcgtc ggcggcgctt ccggggatcc gggccgcgcg
    11461 gtcgtgcagg ccgcactcgc ggtgctcggc gctctcccac caccagcggc cggcgcgcca
    11521 gtcgtcgccc tcggccacgg cgcgcgtgca gggggcgcag ccgatgctgg ggaagccctg
    11581 gtcgtgcagc gggttgtacg gcacgtcgtt ggcgcggatg aactcccaca cgcgggcctc
    11641 gtcccaggac gccagcgggt tgatcttgac catgtcggcg agctggtccc actcggcgat
    11701 ctcgaccttc tgccgcgtgg cgccctggcc gctgcgcagc ccgcagatcc aggcggtcag
    11761 ctcgagctgc gcgcgccgca gcgggaggat cttgcgcacc tcgcagcagc ggtgccgcgc
    11821 ctcgatgctg tcgcggaaga ggttgacccc gtcgcggtcg accatcttct cgacctcggc
    11881 cgctgccggg aagtacgtct tgatgcacag gtcgtagtgc ttggccgtcc ggtcgagcag
    11941 gtcgtaggtc tcggggaaca gccggcccgt gtcgagcgtg aagatgggga tgtcgaggcc
    12001 ggcggcggcg atcatgtgcg tcaggacctg gtcctccgcg ccgagggacg aggcgaacgc
    12061 gacgtggccc gagaagcgct cggccacggc ggccagcacc tcctccgcgg tggcgtcggc
    12121 gagctcgacg atcaggctgg gcacggtctc cgactcgggc ataggggtca cctcagtcaa
    12181 ttgcaattct tggaattgaa gaataacgcg attgcatggt acgacggacc ggccgccgcc
    12241 cgcaactccg tgggggcggc ggcctcagct cggggccgcc gccggccgtc acgcctcgcg
    12301 taccgggctc gagcagggcg cgcagtcgat ggtggcgtag ccctgggcgt gcagcgggtt
    12361 gtagggcacg tcgtgcgcgc gagcgtagtc ccagacggcg gcttcgtccc acgccgccag
    12421 cgggttgatc gtgaccagcc cggcgctcgc gtcccacgcg gccggctcga gcgtctcgtg
    12481 ctcgccggcg ccgccgtggc gcgcgcagat ccagacgtcc aggccgatct gcgcgcggcg
    12541 gagcggcctc accctgcggg tctcgcagca gaggtccctc ttgaacgcgc tgtggcggaa
    12601 gaggttgatg ccgtcgcgcg cgaccatctg ttcgagctcc gccgcgtccg ggcagtagac
    12661 ggcgatgctc acgccgtagc gctcgctcgt gcgttcgatg aggtcgtggg tctcggggaa
    12721 gaggcgaccg gtgtcgaccg tgaagatcgg gatgcccagc ccggcctcgg cgatcatgtg
    12781 cgtgacgacc tgatcgtcga tcccgagtga cgtggcgaac gccaagcggc ccgcatagcg
    12841 gccggcggcg tcggcgagga tcgcctcggc ggggaggtcc cgcagccgct ccaggatgtc
    12901 ggccacaggc tcaagaacgg tcattccgtg cctcccgtcg tgagtcgccg gcgcctgcgc
    12961 acgcgtccgg ccatgcgcga cgaggacgca tgatagggcc cccgcgcggc gagggcaaac
    13021 cgcggaggag cgcgccggtc agcgctcggc cggcgcggcc ggcgccgcgg acgccgcgcc
    13081 gccctcgagc tcgagcagcc gcagcttgcg ccagaggccg ccgccgtagc cgccgaggcc
    13141 gccgtcggcg gcgatcacgc ggtggcaggg gatgacgatg gcgatccggt tggcgccgtt
    13201 ggcggtgccc acggcgcgcg ccgagcccgg cgcgccgatc tcccgcgcga gctgcgcgta
    13261 ggagcgcgtc tcgccgtagg ggatgcgccg cagggcgtcc cagacgcggc gctggaaggg
    13321 gcttccgggg tactcgagcg ggaggtcgaa ggccgtgcgc ctgccggcga agtactcgtc
    13381 cagctgcgcg cgcaggggcg cgaagagagg gctgtctccc ggggcgaggg gccggccgaa
    13441 ccgccgccgg agggtggcga gctggctctc catcatgcgc cggtcggggt actcgagcag
    13501 gcagacggcg tcgtcggtcg cgccggcgac cagggggccg accggggtct gcagccaggt
    13561 gaggcggatg agggagccgt ccggcgccgc cgccgcttcg ccgggcggcg cccccgccag
    13621 cttgccgaag gccgtgcgaa accctgagtg cgagccccag ccgtggtcga agacgacctc
    13681 gtcgatgacg ccgccggagc ggagcgcggc gaaggcgtcg ccgagccgcc gcgcgcggca
    13741 gtaggcctgg aaggtcatgc cgtgcgtcct gcggaaccag cggcgcacgg cgacggggtc
    13801 caggccctcg gcgcgcaggt ccgcgtcgct gatgcgcgca gagggggagt cctcgacgcg
    13861 cgcgatcagc cgcgaggccc actcgggggc gccggcctcg gcgtcgagcg gccggcaccg
    13921 cttgcacggc cggaagccgg cgacgagcgc ctcacgcgtg gtcgcgtaga actccacgtg
    13981 ctccggcttc gggtctcgcg cggggcacga cgggcggcag aacacgccgg tggtggtcac
    14041 ggcggcgtag aagaggccgt cgtaggagcg gtcgcgctcg cgcagcgcgg tgagcatctc
    14101 ctcgcgcggg agcagcgggc ccgcgggccg ccgccggtgc gctctctgtg acgtcgcgtc
    14161 catgtctgag agagtagtgc ccgttgcgcg cggcggccac cgaattctgg acgccgaatc
    14221 cgttctgcgc gcccggcgcg ctcagtccgc gacggtcacg ttcgcgtcgg gatcaagcgg
    14281 gttggtgcgc acggccaggg agaacaggaa cgggtactgc gtctgcaggt gctgcaggta
    14341 gtcgatccac agcaccccca gcagcgtgta ggcgcgcttg acgtccgcga cgaggtgggc
    14401 gcggtccgcc tgcggcaggt ggtcgaagtc gacgcgcgcc tgcagttcct ccgcgaggtg
    14461 cgtcaccgcc cagagcaggt cggtgaacgc ctcgtgctcc atgagcgcct ggttgccgag
    14521 gaggctgagc aggtacgtct tctccttgtc gagccgctcg cgcagcgcgg ccaggtcccc
    14581 gccgctcagc tcgatgaccg gctcgtgcgc ccggaacgcc tgcttggctc tctcgtagtc
    14641 cttcgccgtc cacgtcccgc gcgccagcag gtcctcgcgc accttgtcca gctcccggtc
    14701 cagcctggcg atgcgaccca ggacgtccgt gccgcactcg ctgaagaacg ccccgacgat
    14761 catgttgagc ttgtcgagca tggcctcccg ctgccggcgc tgcatgaggc cgtcgatcag
    14821 catggagacg agcaggacct ggatgaacag gaacgcgatg tcgtcgacca gatagcgcag
    14881 catctcgtcg tggaagctct cggaggggta gaggatccac cgcagggcgt acacgacgat
    14941 ggcggcgacg atgagcgtgg cggcgatctc gacttgccag cggcgcaggc gcatgcggtc
    15001 tccggggtgg ggcaggggtg cgggctagga taccggaccg tacgcgcgtg tgcagcaggt
    15061 cgtttcctcc gatgggatcg ccttgtactt caccaggcgt tctcgtcgaa tgacccttgc
    15121 ggatcgctca ggtcggcgcg gctgatcggg cgtcgcttcc ctgtagctac gtgcctgctg
    15181 cacagaaaga agtattcccc gccttctggt gcgtcacgcc gttgcgaccg cgctcacggc
    15241 gcggcgtgcc agggcttctt gcgcagcacc ctcggccgca ggtaggccat cacggccggc
    15301 cagtcgggga acttctccat cccgaagtgg aggtgcttgc cctcgaagcc gtccgctccg
    15361 ttcttggtgc ggtcgtcgat gaggaagtca ccgcggtgca gctccttgtg atgcgtgagg
    15421 atgaggcgct tgtacgccgg ttcgccgagg tgtgtcttga cccagcagag cttgtccgac
    15481 caggccgacg ggttgtccca cggcgacgtc gagaggatgt aggtgtcgaa gagcagcgcc
    15541 agttcggtga acgcgcagag cgcgcccggc atggggtcca tgagggagaa gatgccgggc
    15601 acgtcgtcca agtggtccgc atactgcttg cggaccttct tcttcaggtg cggaagcgcc
    15661 gagcggaagt cgacgagcac gttgtccatg tccacgtaga cgatcttctt cgtcatggcg
    15721 ccctcccggg gtggcttgct tcgactctat cccgccggca gggcggcggg ccaggctcgg
    15781 cggtcgtcgc cctgtcgcac gcatcgtcat cttgtggctc gcggcgaggc cggtcagtct
    15841 gcggcgaacg agttggggcg tcgatacttg atggtatttg gcgcgcgtcg cagtggagcc
    15901 cgtggccatc gagaagaacg ggcgcccggt ggccgtgctg ctctcctccg aagagtacga
    15961 actgctgcaa cgctccgacg acttcttctg gggccaagcc gcgcgtgcgg cggaggccga
    16021 gggcttcctc agtgccgagg actctctcag gtacctgcag gacggggcgt ccgccgaaga
    16081 ccgtgctgcc tcttagtctg tcgaaagcgt cggtccgctt tctcgaaacg ctgccgccca
    16141 aacagtaccg gcaggtcgcg cgcaagatca tcatgtcgat ggccgatccg cggcccgcgg
    16201 atgccgagcg tctgaagggc tacgagtact cgcgcgtcac ggtgggcgag taccgcatca
    16261 tcttcgacgt ccgcgacggg ctgctgcgcg ttctcgcgat cggcaagcgc aacgacgacg
    16321 aggtgtaccg gcggctgagc tgaggccgcg tctgggatca cgtggcggag agggggggat
    16381 tcgaaccccc gaaccgagag gacccggtta acggttttcg agaccgccgc attcaaccgc
    16441 tctgccacct ctccgcgagg cggcggccgc cctcagagtc aggaggccgc gggagcgtag
    16501 tctagcatgc gcccgcggct cggctcacgc gccgcggatg cgccgggcaa acgcgctggt
    16561 gcgcgcggac gcgccggtca ctcggcgagg cggtcctcca cccacgcgcg gacgtcctcc
    16621 ggcgagaagc cgtactggtc tttgacatcc ttcatcggca ggccgagggc ctcggcgggc
    16681 acgccccact cggcgctgaa ctcctgcagg gggatgcccg tcatcgcggc gatgtccgcg
    16741 aaggacatcc agcccttgat cggcgagtcg ctgccgccct ggccctgtcc ctcgcctccg
    16801 cctgagtgcc cggcgccgcg ggcctcggcc cggccctcca tctgccagtc gaacgcgccg
    16861 gtgagcgtgg tcacaccgac gatggccacc atcagcgcca ccaccgcgcc ggtcgcgacg
    16921 agcgccgtgg cgcgggcgcc gccgggggcc gtcaccttca aggcgccggg cacggggcag
    16981 gcgttgacgc actcgttgca ggagatgcac tcggagctgg tgaccgcgtc ggcggtcgcc
    17041 acctcgacgt tcatcatgca gaccttgtcg cagcggccgc agtcgatgca ggcgtccgcg
    17101 tccc
//
LOCUS       DASUMX010000279         2033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1108039, whole genome shotgun sequence.
ACCESSION   DASUMX010000279 DASUMX010000000
VERSION     DASUMX010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1108039"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            209..1501
                     /locus_tag="VFZ86_13290"
     CDS             209..1501
                     /locus_tag="VFZ86_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HEX5643290.1"
                     /translation="MRERAVRRMAGVLAGRLPELGLGEVPDPRARAGRWKLPQILGAT
                     LLGLMAGCKSLAESEALTETLAPAMRRRLGIPRRLADTTARDALCRVPLDGLRAVLRR
                     AVRAAWRRKALGPVRLPMSVVAMDGKATSLPTLNHPLVQNHIEGQEVPFGMVRTVTCT
                     LVSAAGRPCLDAVPIPGGTNEVGHFQVAFRRLVKHFGKLFDFVTYDAGGFSRANADAV
                     IAAGKDYLFALKDEHRTMCRLADEHLQAEAVIDTTEDVLDNGTTVVRSLKLLRADPSW
                     AYGDGKSPGESVWQHAKAFLSVEYVRVKGRVIVERDERMFVSSYDPKRLTPQQWLLLV
                     RSHWGVESNHHTLDTAFAEDDRPWIEADPNGMLAVLLLRRVAYTLLALYRAVTLRSDE
                     GRTLSWKALLQSLRDVLIAATEDQLAGLRLHEVAAATR"
     gene            1791..>2033
                     /locus_tag="VFZ86_13295"
     CDS             1791..>2033
                     /locus_tag="VFZ86_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643291.1"
                     /translation="MAVKVMVAPAIVKGQVARAPVSASALHPHVKEAPKPKRRKSAVY
                     DADGSEVFITLMCLKCHKMKPLSQFGLRKMADGAIRN"
BASE COUNT          300 a          679 c          778 g          276 t
ORIGIN      
        1 cggccgacgc tccccgatag gtgacggggg gttggctcgg cggggccggg cggcggggcg
       61 cgtgcacaaa ttcgcagccc catctccact cgcggtacga agaggtgcgg cgggcgatcc
      121 gtgaggggtc gcccgccgcg gttcggcttg tcgtcgggta ggagcccggc ggatgccaac
      181 gaccccttga tcgtactgga ggagatccgt gagggaaagg gctgtgagga ggatggcggg
      241 ggtcctggcg gggaggctgc cggagctggg actcggggag gtgcccgacc cgcgggcgcg
      301 ggcgggacgc tggaagctcc cccagatcct gggggcgacg ctgctggggc tgatggcggg
      361 atgcaagagc ctcgcggaga gcgaggcgct caccgagacc ctggcgccgg cgatgcggcg
      421 gcggctgggc atcccccggc ggctggcgga cacgacggcg cgggacgcgc tctgccgggt
      481 cccgctcgac gggctgcggg cggtgcttcg gcgtgccgtc cgcgcggcct ggcggcgaaa
      541 ggcgctcggg ccggtgaggc tgccgatgag cgtcgtcgcg atggacggga aggcgacgtc
      601 gctgccgacg ctcaaccacc cgctggtgca gaaccacatc gaggggcagg aggtgccgtt
      661 cggcatggtg cgcaccgtca cgtgcacgct cgtctcggcg gcggggcggc cgtgcctcga
      721 cgccgtgccg atcccgggag ggacgaacga ggtgggccac ttccaggtgg cgttccggcg
      781 gctcgtgaag cacttcggga agctcttcga cttcgtcacc tacgacgccg gaggcttctc
      841 ccgggcgaac gccgacgcgg tgatcgccgc gggcaaggac tacctcttcg cgctgaagga
      901 cgagcaccgg acgatgtgcc ggctcgccga cgagcatctc caggctgagg ccgtcatcga
      961 cacgaccgag gacgtgctcg acaacgggac caccgtcgtc cgctcgctca agctgctccg
     1021 ggccgacccg agctgggcct acggcgacgg gaagtcgccg ggggagtcgg tctggcagca
     1081 tgccaaggcc ttcctctcgg tcgagtacgt gagggtgaag ggcagggtca tcgtcgagcg
     1141 ggacgagcgg atgttcgtct cgagctacga cccgaagcgg ctgacgccgc agcagtggct
     1201 gctcctcgtg aggagccact ggggcgtcga gagcaaccac cacacgctcg acaccgcctt
     1261 cgccgaggac gaccggccgt ggatcgaagc cgacccgaac ggcatgctcg ccgtgctgct
     1321 gctccggcgc gtcgcgtaca ccctgctcgc gctctaccgc gccgtgacgc tccgctcgga
     1381 cgagggccgc accctcagct ggaaggcgct gctccagtcg ctgcgcgacg tgctcatcgc
     1441 cgccaccgag gaccagctcg caggcctgcg cctgcacgag gttgccgccg cgacacgctg
     1501 aaatcgccgg gctcctaccc gcggcgggtg acattgaagg gacctgaggg agcggccgac
     1561 gcgacgggcg ccggctcgcc gtggctgccg cttccctcga cgcccggccg accgacctcc
     1621 gtcgcccccc ggcggagggg gcgaagcctg cccccgtgcc ggccgatcac acgtggtggc
     1681 gcgcggcggg tcgcgggcgg atgtcagagg ggggggctag cctcaggctg ccgggcatcc
     1741 tttccgtcgt gcccaggcga ggttgctccg caaagcagga ggtagaagcg atggccgtca
     1801 aggtcatggt cgcccccgcg atcgtgaaag ggcaggtcgc ccgcgcgccg gtgagcgcgt
     1861 cggcgttgca tccgcacgtg aaggaggccc cgaagccgaa gcgtcggaag tcggccgtct
     1921 acgacgccga cgggagcgag gtcttcatca cgctgatgtg cctcaagtgc cacaagatga
     1981 agcccctctc ccagttcggc ctgcggaaga tggccgacgg cgcgatccgg aac
//
LOCUS       DASUMX010000280         3532 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_966295, whole genome shotgun sequence.
ACCESSION   DASUMX010000280 DASUMX010000000
VERSION     DASUMX010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3532
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_966295"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            274..762
                     /locus_tag="VFZ86_13300"
     CDS             274..762
                     /locus_tag="VFZ86_13300"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX5643292.1"
                     /translation="MSSARQSVDVRVDVKTAYAQWARFESYPQWMEGVASIDELGDGR
                     LHWVAKVKDEVATAENEVREWDARITEQVPDECISWESVPGRPEEKPNAGRVTFEPLG
                     TSACRVTFGIDWEPEAGLEASEAYVLDAVNQMLAADLARFKNLIEARAAAPRRSGGEL
                     AA"
     gene            complement(780..1316)
                     /locus_tag="VFZ86_13305"
     CDS             complement(780..1316)
                     /locus_tag="VFZ86_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HEX5643293.1"
                     /translation="MYAVGDNVVYPCHGAGKIVKIEQRAVLGRRREYLTIELHHNRMT
                     ALIPVENAERAGLRKVVAADAVDEVLQVLRDAPTAMPEKWHARSKRIQEKLGTGDILE
                     VAQVVRSLAVRQEGKALPTGEKQLFSRAQHILASELMYARGLTAEEAESLLEGVLEGA
                     RADAPAALAAVREPEPVA"
     gene            1569..2660
                     /locus_tag="VFZ86_13310"
     CDS             1569..2660
                     /locus_tag="VFZ86_13310"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX5643294.1"
                     /translation="MRSFRAGLRQRSPLVLVVAAGLAALTLLGLQQAFATGAWSEVPL
                     EYWMRSDNVDTGKARVAWLLARLDREPAAERTLYLLGGSTLREATVRDGASLAAKVED
                     LGGPHLEGWNLATSLQTFAQSLAIVDNLPASGSATVVIGVSPGRFIGDRAGAEEQLEG
                     RGFPLGSETLGGCLDAEPSAGDPRGTLLPGILSMLVEKGRSDVVRLAQGEVPSWDYVQ
                     HDFDDEPLMDEAEKRGVFEAWWRVRLPQFERNVDFNLTLLEALVERSRRRGFGVVVVE
                     LPRNEELIGARWEAVDSRYQPRARSIAAEHGVPYLDFNGSLGLHTADFSDHAHLRHSG
                     RIVWETQLAQELALLYQRGALPDRSVAGN"
     gene            complement(2764..2913)
                     /locus_tag="VFZ86_13315"
     CDS             complement(2764..2913)
                     /locus_tag="VFZ86_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015540068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5679 domain-containing protein"
                     /protein_id="HEX5643295.1"
                     /translation="MATEAYCVKCRTKRTMKDEKAVTMKNGKPATQGVCPECGTKMFK
                     IGKSK"
     gene            complement(3022..>3532)
                     /locus_tag="VFZ86_13320"
     CDS             complement(3022..>3532)
                     /locus_tag="VFZ86_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008053551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase subunit alpha"
                     /protein_id="HEX5643296.1"
                     /translation="AEPRLGGPDNVIAGNTCLYGATGGRFFAAGRAGERFAVRNSAAT
                     AVVEGAGDHCCEYMTGGVVAVLGGVGRNACAGMTGGAAYFLADAAPAWLAGHPTVAAA
                     DMDEASVAELTALLEAHAARTGSEVAASLLADPETLAGRFVRVRPARAPARLAGPPRP
                     SGTASVARA"
BASE COUNT          477 a         1269 c         1255 g          531 t
ORIGIN      
        1 gcgcacaggc cggcgatgcc gccgccgacg atcgccaggg acttgcccgt ggtagctgct
       61 ccgttgactg gcatgaggac ctccggcggt cggcgggtcg aggccgcgag cgcgtggccg
      121 gctcgctgcg gcgactctgc tttgtgtcac agggcgccgt caagccattt cgcgcccgtc
      181 acgctcgcag gcgacggcac cgcgcggtgg gttcgtggga cgcaggaccg ggaacgctaa
      241 gatatccccg aatatagtag gagttgatcc tccatgtctt ctgcacgaca gtccgtcgac
      301 gtccgcgtcg acgtgaagac cgcctacgcc cagtgggccc ggttcgagag ctatccccag
      361 tggatggagg gagtcgcctc gatcgacgag ctcggcgacg gccgtctcca ctgggtcgcc
      421 aaggtcaagg acgaggtcgc taccgctgag aacgaagtcc gtgagtggga cgcccggatc
      481 accgagcagg tccccgatga gtgcatctcc tgggagagcg tccccgggcg cccggaggag
      541 aagccgaacg ccgggcgcgt caccttcgaa ccgctcggca cgtcggcgtg ccgcgtcacg
      601 ttcggcatcg actgggagcc cgaggcgggc ctcgaggcca gcgaggccta cgtgctcgac
      661 gcggtgaacc agatgctggc ggcggatctc gcgcgattca agaacctcat cgaggcgcgc
      721 gcggcggcgc cacgacgctc aggcggcgag ctcgccgcat aaggcgtcgt ccgcgcgtct
      781 caggcgacgg gttccggctc tctgaccgcc gcgagtgccg ccggtgcgtc ggcccgggcg
      841 ccttcgagca cgccctccag gaggctctcg gcctcctccg cggtgaggcc gcgcgcgtac
      901 atcagctcgc tggcgaggat gtgctgggcg cgcgagaaca gctgcttctc gccggtgggc
      961 agagccttgc cctcctgccg cacggccagg ctgcggacca cctgggcgac ctcgaggatg
     1021 tcccccgtgc cgagcttctc ctggatgcgt ttggagcgtg cgtgccactt ctcgggcatc
     1081 gccgtaggag cgtcgcgcag cacctggagc acctcgtcga cggcgtcggc cgcgacgacc
     1141 ttgcgcagac ccgcgcgctc cgcgttctcc acggggatca gcgcggtcat gcggttgtga
     1201 tgcaactcga tggtgaggta ctcgcgccgc ctgccgagca ccgcccgctg ctcgatcttg
     1261 acgatcttgc cggcgccgtg acacgggtac acgacgttgt ctccgactgc atacatctca
     1321 gctctccctg cctctgtgct gggcgccctt gcggccggct cgcgtggcac gccctcggca
     1381 tggagggagg cgacaacccg ctcacccgaa ggtgcggcgg tctgacttct gcctgctatt
     1441 gtacaggaaa cgaccccgtc tttcactttg gcgccgccgg cgatagcctt gtgccgaccg
     1501 gttctcagac cagaagacga ggctgacggg tgaaggcctg cgggacagag cagaggggcg
     1561 cgggcttcat gcgaagcttc cgcgcgggcc tgcggcagcg cagcccgctg gtgctcgtcg
     1621 tcgccgccgg cctggcggcg ctcacgctgc tggggctcca gcaggccttc gcgaccggcg
     1681 cctggtcgga ggtgccgctc gagtactgga tgcgctcgga caacgtggac acgggcaagg
     1741 cgcgcgtggc ctggttgctg gcgcggctgg accgggagcc ggccgccgag aggacgctct
     1801 acctgctcgg cggctccacc ctgcgcgagg cgaccgtccg cgacggcgcc tcactcgccg
     1861 ccaaggtcga agacctcggc gggccgcacc tcgagggctg gaacctggcg acctccctcc
     1921 agaccttcgc ccagagcctc gccatcgtcg acaacctgcc cgcgtccggg tcggcgacgg
     1981 tcgtcatcgg cgtcagcccc ggccgcttca tcggcgaccg cgccggcgcc gaggagcaac
     2041 tggaggggcg cggcttcccc ctcggaagcg agacgctcgg cggctgcctg gacgcggagc
     2101 cctccgccgg ggatccacgc gggacgctcc tccccggcat cctcagcatg ctggtcgaga
     2161 agggccgctc cgacgtcgtc cggctggcgc agggcgaggt cccgtcctgg gactacgtgc
     2221 agcacgactt cgacgacgag ccgctgatgg acgaggccga gaagcgcggc gtgttcgagg
     2281 cgtggtggcg cgtgcgcctg ccgcagttcg agcgcaacgt ggacttcaac ctcaccctgc
     2341 tcgaggcgct cgtcgagcgc agccggcggc gcggcttcgg cgtcgtcgtc gtggaactgc
     2401 cccgcaacga ggagctgatc ggcgcacggt gggaggccgt cgactctcgc taccagccgc
     2461 gggcgcggag catcgccgcg gagcacggcg tgccctacct cgacttcaac ggcagcctcg
     2521 ggctgcacac cgccgacttc tccgaccacg cccacctgcg ccactcgggc cgcatcgtgt
     2581 gggagaccca gttggcacag gaactggcgt tgctctacca gcggggcgcg ctgcccgacc
     2641 ggagcgtggc ggggaactga cggagcatcc tggtcgcgta cgcggctggg gccggggcgc
     2701 tctcacgccc cggccccagc cgcttggctc tcgaacggcc ctctcagccg ggccggatca
     2761 ctcttacttg ctcttgccga tcttgaacat cttggtgccg cattccgggc acacgccctg
     2821 cgtcgccggc ttgccgttct tcatcgtgac ggccttctca tccttcatgg tgcgcttggt
     2881 gcggcatttg acgcagtaag cctcggtcgc catgcggtct cctttcggga cacgtcgcgg
     2941 gccccacgcc cgcccccctg atttcgccgg gagcatactc cctcgcctga acgtatgcaa
     3001 cgagcgtgcg ttgagcctgc gtcacgctcg cgccacgctc gccgtgcccg agggccgcgg
     3061 cggtcccgcg aggcgggccg gagcgcgcgc cggacggacg cggacgaaac ggccggcgag
     3121 ggtctccggg tcggccagca gcgaggcggc gacctcgctg ccggtgcggg ccgcgtgcgc
     3181 ctcgagaagc gcggtgagct cggccacgga ggcctcgtcc atgtccgcgg cggcgaccgt
     3241 cgggtggccg gcgagccacg cgggcgcggc gtcggcgagg aagtacgcgg cgcccccggt
     3301 catcccggcg caggcgttac ggcccacgcc gccgagcacc gcgaccacgc cgccagtcat
     3361 gtactcgcag cagtggtcgc cggcgccctc caccacggcc gtggccgccg agttgcgcac
     3421 cgcgaagcgc tcgccggcgc ggccggccgc gaagaagcgg ccgcccgtcg cgccgtacag
     3481 gcaggtgttg ccggcgatga cgttgtccgg gccgccgagg cgcggctccg cg
//
LOCUS       DASUMX010000281         7710 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_534637, whole genome shotgun sequence.
ACCESSION   DASUMX010000281 DASUMX010000000
VERSION     DASUMX010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 7710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7710
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534637"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..973
                     /locus_tag="VFZ86_13325"
     CDS             <1..973
                     /locus_tag="VFZ86_13325"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643297.1"
                     /translation="SRRPVLFPTHDQYIWPISRNAARLEKWYLVPFSRWETMQRLHDK
                     RAQMEAAWRAGVDTPKTVFVDSADDLERGVEEVGFPCIFKPVESLAFKTRFRRHVLEI
                     GSKDELLRTYDAVRDCGTLMLQDIVPGGDEELYTLGSYLDAQSRPLAQFTGHKLRQHP
                     PRFGHVSMAVSTWVPELAEAGLRLLHELGYHGVSQVEFKRDPRDGRFRLMEVNARHWM
                     WHSLATACGVNLSLSAYRDAIGEPYISPRQVDGLKWVVSLTDARDAFARWRRGEEKLW
                     PWLRSYRGVRVDGLYSLRDPLPGALLTARQAKTIVTRGGRPAEGESP"
     gene            970..2184
                     /locus_tag="VFZ86_13330"
     CDS             970..2184
                     /locus_tag="VFZ86_13330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643298.1"
                     /translation="MRMGRFQAAAAVRPPAAVFQVGYACGLDILRDLGRHGVPLLAFD
                     PDPGAIGLRSRYAAARVCPDPLADEEAFVTWLETVGPSLPQRAVAFPSHDEFIWPLSR
                     HAARLEPWFIVPFSRWEVMSRVHDKRAQMEAAWRAGVDTPKTVFVGSGAELEAAAGEV
                     RFPAVLKPVESLAFKLRFHRHILDVETPAELRRIYDKVDDLGVLMLQERIPGGEEELW
                     TVGSYLDAESRPLAVFTGHKLRQYPHAGGSALAGVSRWDGELADAGLRLLRELRFHGV
                     SQVEFKRDPRDGRFRLMEVNARHWKWHGLAARCGVNLSYVAYRDAIGDPYLARRQQDG
                     VKWIVVNKDVPLALMEIARRRRSAPAYVRSLRGTRMDGLHALDDPLPGLLNAGSAVKQ
                     VLTRAPSSREDV"
     gene            2282..3751
                     /locus_tag="VFZ86_13335"
     CDS             2282..3751
                     /locus_tag="VFZ86_13335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX5643299.1"
                     /translation="MTLPELQVYVGAPDAFAPRAEWILRTLLAPLGRGATFTRDPARA
                     GGSVLAYAAGPVAGVPTIPCDAEAMALIAAERPLPAGAFAARASGDGAAVAAWPAGPE
                     AGFAVPFDLVASAFVLLACWDERTTGERDKYGRLPFAASVFAANPALTIEEPAVDRYA
                     ELVRAALAPRLAELGLEPLQAAGSVWGARGRCAIALTHDLDNLWRWTRRGFAAAGYRT
                     ARAARRLQGRAVARELGDGADWLVRHLPRRTDPFWTFPQILSGEDARGVSSTFYVIAR
                     HTHKQDGNQPATYAKRIPEALRLVTEAGREVGLHGNDADRLALAEIVRDRDDLSARAG
                     RAVSGIRYHYLRALYHETLPLLEQAGLTYDTTLAFAEREGFRCGCSFPFQPYSLDDER
                     PLDLVELPLAVMDGTLQEPHYRGLAAADAERAAASVLARALRSGGAVSLLWHNNRFDK
                     RVSRGYDRVYWDLVDQALARGAWCTSAADVVARWKEATA"
     gene            3748..4881
                     /locus_tag="VFZ86_13340"
     CDS             3748..4881
                     /locus_tag="VFZ86_13340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5643300.1"
                     /translation="MTRVLHLSVVHKPDDPRIHERECRTLAQAGYEVMYMVPGAVPGR
                     DDAGVILAPLPERGRSTRFLSSVEITRALRLLKPDVLHVHDPELLTLFPAARAFVPRL
                     VYDMHEYVPEAVAGKPYLPARIRPAASQTTAVAQRSLAALGSGVVVVTDDQLAALGGT
                     PKLRLVLPNYPRVERFEGAAPVAELAADPRLKLIYVGSLSRARGCTMMLDVMEQVEPD
                     EAVLYLGGTFNDPALELEVGERLALGLADRVKLLGRVPPPELPRYLAAADVVWVPSQP
                     DRQYSHPTIPTKLLEGMTMGLAALVSDMPGRGELVRLEKCGLAVEPGAEGHLRGLRRL
                     LMDRRALPEMGARGRRAVERRYSWEAVQGDLVDFYGSLCAGLP"
     gene            complement(4903..5733)
                     /locus_tag="VFZ86_13345"
     CDS             complement(4903..5733)
                     /locus_tag="VFZ86_13345"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5643301.1"
                     /translation="MDVVDFRVRLRTPHMLGPWNPDDPAPHFSQYIELYNMRPRLTPL
                     GMAEFVAMMRSEGVSRGVVCGGSIADNDHIMEVRDSLGDDDPFHYIAGVHPSNGVRRN
                     LEELERCRQAGFLGVNVSPFIWGIPANASVLYPIYAYCESNNLVAIVHGSLHYNRFQS
                     MWLGDPQYMDEIAMNFPSLKLVISHAGNGFGVLALAVAQKHPNIFLEFSALWPKYLPE
                     ATIQAVNSYLKDRCLFGTDYPLVEFGRAVEAWTEAVKPAVRELFFAKNAERCLFGEPR
                     "
     gene            complement(5760..6899)
                     /locus_tag="VFZ86_13350"
     CDS             complement(5760..6899)
                     /locus_tag="VFZ86_13350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643302.1"
                     /translation="MRTVSRTYQPGDEAQINRLYLSLTGRDRTPEEFAWEWLETWDGQ
                     GSMNLVFDLDREEGDQLVAQYSLIPTPLSVWGRPALAGKTENCMSHPDHRGTGLYSAH
                     ERECFEREKERYSFFFTTAGTVSAGAVGRVRSKLGYVAFDDWANYTLWLKTSVLREDI
                     GSILRAKGAKRLAPVLSSLLAGALQVYSHARRVRSCRFGVAVHDAADAPLEAIADLWE
                     RNRRRYGITVDRSAAYLRWRVNDDPHVEHKYLTMHGDDGLLGYAIAFVQEGDLHVVDV
                     LVDGARADLFDHLFGALTRWGKELRLSRVKCLALRRSALLPRRLSRAGFVDSSLFTRG
                     GLFKRPAPRQFYLYIPEELRGEPAVTGNADWYITSLVLEGLPRSN"
     gene            7025..>7710
                     /locus_tag="VFZ86_13355"
     CDS             7025..>7710
                     /locus_tag="VFZ86_13355"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5643303.1"
                     /translation="MDGAQDRADARAGMVEGRLLKVLVVSHLYPWPEAERHLFVHQQV
                     LALKEIGVESRVLSPTPWAPRALWRSERQRRRGRKPREAVRDGVVAEYPRVLQPPRHL
                     LFDRLGDLAYRTVRRLPSVRSAGYDLVHAHQALPDGALAQRLAADLGVPVAITVHGAD
                     VYQHLRMGGAVERRVRQVLGGADAVMANSSVVARLLRDVVAPERLSVVLNGTTGLEEQ
                     VEPASDYLPG"
BASE COUNT         1035 a         2863 c         2713 g         1099 t
ORIGIN      
        1 ctcccgccgc ccggtgctgt tccccacaca cgaccagtac atctggccga tatccaggaa
       61 cgccgcgcgc ctcgagaagt ggtatctcgt ccccttctcg cgctgggaga cgatgcagcg
      121 gctgcacgac aagcgcgccc agatggaggc ggcctggcgc gcgggcgtcg acacgccgaa
      181 gacggtgttc gtcgactccg ccgacgacct cgagcgcggc gtggaggagg tcgggttccc
      241 ctgcatcttc aagccggtgg agtccctcgc cttcaagacg cgctttcgcc gccacgtgct
      301 ggagatcggc tcgaaagacg agctgctgcg cacgtacgac gcggtccgcg attgcggcac
      361 gctgatgctg caggacatcg tgcccggcgg cgacgaggag ctctacacgc tcggctcgta
      421 cctcgacgcg cagagccgac cgctggcgca gttcacggga cacaaactgc gccagcaccc
      481 cccgcggttc ggccacgtga gcatggccgt gagcacgtgg gtccccgagc tcgcggaggc
      541 gggactccgc ctgctccacg agctcggata ccacggcgtc agccaggtcg agttcaagcg
      601 cgatccgcgc gacggccgct tccgcctcat ggaggtcaac gcgcgccact ggatgtggca
      661 ctcgctcgcc accgcctgcg gcgtcaatct ctctctctcc gcctaccgcg acgccatcgg
      721 cgagccgtac atctcgcctc gtcaggtcga cggcctcaag tgggtcgtct cgctcaccga
      781 cgcgcgcgac gcgttcgccc gctggcgcag gggcgaggag aagctctggc cgtggctcag
      841 gagctaccgc ggcgtgcgcg tggacgggct gtactcgctg cgcgacccgc tccccggcgc
      901 gctgctcacc gcgcgtcagg cgaagaccat cgtgacgcgt ggcggcaggc cggcggaggg
      961 ggaatcgccg tgaggatggg gcggttccag gcggcggccg cagtccgccc gccggccgcc
     1021 gtcttccagg tcggctacgc ctgcggcctc gacatcctcc gcgacctcgg ccgccacggc
     1081 gtccccctcc tcgccttcga ccccgacccg ggcgcgatcg gcctgcgctc gcgctacgcc
     1141 gccgctcgcg tctgccccga cccgctcgcc gacgaggagg ccttcgtcac ctggctcgag
     1201 accgtcgggc ccagcctgcc acagcgcgcc gtcgccttcc ccagtcacga cgagttcatc
     1261 tggccgctct cgcgtcacgc cgcacggctc gagccgtggt tcatcgtgcc cttctcgcgc
     1321 tgggaggtga tgtcgcgggt ccacgacaag cgcgcccaga tggaggccgc ctggcgcgcc
     1381 ggcgtcgaca cgccgaagac ggtgttcgtc ggctccggcg cggagctcga ggcggccgcc
     1441 ggcgaggtcc gtttcccggc cgtgctcaag ccggtcgaat cgctggcgtt caagctccgc
     1501 ttccaccgcc acatcctcga cgtcgagacc ccggccgagc tgcggcgcat ctacgacaag
     1561 gtcgacgacc tcggcgtgct catgcttcag gagcgcatcc ccggcggcga ggaagagctc
     1621 tggaccgtgg gctcctacct ggacgcagag tcgcggccgc tggccgtctt caccgggcac
     1681 aagctgcggc agtacccgca cgccgggggc agcgcgctcg ccggcgtgag ccgctgggac
     1741 ggggagctcg ccgacgcagg cttgcgcctg ctgcgggagc tgcgcttcca cggcgtcagc
     1801 caggtcgagt tcaagcgcga cccgcgtgac ggccgcttcc gcctcatgga ggtcaacgcg
     1861 cggcactgga agtggcacgg gctggccgcg cgctgcggcg tcaacctctc ctacgtcgcg
     1921 taccgcgacg cgatcggcga cccgtacctc gcccgccgcc agcaggacgg ggtgaagtgg
     1981 atcgtggtca acaaggacgt gccgctggcg ctcatggaga tcgcccggcg ccggcggagc
     2041 gctcccgcgt acgtgcgctc gctgcgcggc acgcgcatgg acggcctgca cgcactcgac
     2101 gacccgctgc cgggcctgct caacgccggc agcgcggtca agcaggtgct gacgcgcgcg
     2161 ccgagctcga gggaggacgt gtgaatagtc ggcgtagcga agctatcgac cggcgcgacg
     2221 tagtggtgcc ggtccgcggc cgcgcgccgc ggcacgggcg gccccgcggg ggagcgcgcc
     2281 cgtgaccctc cccgagctgc aggtgtacgt cggtgcgccc gatgcgttcg cgccccgcgc
     2341 cgagtggatc ctccgcaccc tgctggcgcc gctgggccgc ggtgcgacgt tcacccgcga
     2401 cccggcgcgg gcgggcggct ccgtgctggc gtacgcggcc ggcccggtgg ccggcgtccc
     2461 gacgatcccc tgcgacgccg aggcgatggc gctgatcgcg gcggagcgcc cgctgccggc
     2521 cggcgccttc gcggcgcgag cgagcggcga cggcgccgcc gtcgccgcct ggcccgccgg
     2581 ccccgaggcc ggcttcgccg tccccttcga cctcgtggcc tccgcgttcg tcctgctcgc
     2641 ctgctgggac gagcgtacga ccggcgagcg cgacaagtac ggccggttgc cgttcgcggc
     2701 gagcgtcttc gccgccaatc cggccctgac gatcgaggag ccggcggtgg accgctacgc
     2761 cgagctggtg cgcgccgccc tggcgccgcg cctcgccgag ctcggcctcg agccgctcca
     2821 ggcggcaggg agcgtctggg gagcgcgcgg ccgctgcgcg atcgcgctca cccacgacct
     2881 cgacaacctg tggcggtgga cgcggcgcgg cttcgccgcc gccggctacc gcaccgcccg
     2941 cgcggcccga cgcctccagg gccgcgccgt cgcccgcgag ctgggcgacg gcgccgactg
     3001 gctggtccgt cacctgcccc gccgtaccga ccccttctgg accttcccgc agatcctctc
     3061 cggcgaggac gcgcgcggcg tctcttcgac cttctacgtg atcgcccgtc acacgcacaa
     3121 gcaggacggc aaccagcccg caacgtacgc caagcggatc cccgaggcgc tgcgcctggt
     3181 cacggaggcc gggcgcgagg tgggcctcca cggcaacgac gccgaccggc tggcgctcgc
     3241 cgagatcgtg cgcgaccggg acgatctgtc cgcgcgcgcg ggtcgcgccg tcagcggcat
     3301 ccgctaccac tacctcaggg ccctctacca cgagacgctg ccgctgctgg agcaggcggg
     3361 actcacctac gacaccaccc tggcgttcgc cgagcgcgag ggcttccgtt gcggctgctc
     3421 gttcccgttc cagccgtatt cactcgacga cgagcggccc ctcgacctcg tcgagctgcc
     3481 gctcgcggtg atggacggca ccctgcagga gccgcactac cgcgggctgg cggcggccga
     3541 cgccgagcgc gccgccgcct ccgtgctcgc ccgcgccctg cgcagcggcg gcgcggtcag
     3601 cctgctgtgg cacaacaacc gcttcgacaa gcgcgtctcg cgcggctacg acagggtcta
     3661 ctgggacctc gtcgaccagg cgctggcgcg cggcgcctgg tgcacgagcg cggccgacgt
     3721 cgtggcccgc tggaaggagg cgacggcgtg acccgggtcc tccacctgag cgtcgtccac
     3781 aagcccgacg acccgcgcat ccacgagcgc gagtgccgca cgctggccca ggccggctac
     3841 gaggtgatgt acatggtccc cggagcggtg ccgggccgcg acgacgccgg cgtcatcctg
     3901 gcgccgctgc cggagcgcgg ccgcagcaca cggttcctga gctccgtgga gatcacccgg
     3961 gcgctgcgcc tgctcaagcc ggacgtgctg cacgtgcacg acccggaact gctcacgctg
     4021 ttccccgcgg ccagggcgtt cgtgccgcgg ctcgtctacg acatgcacga gtacgtgccc
     4081 gaggccgtcg ccggcaagcc gtatctgccg gcgaggatcc gtccggccgc ctcgcagacc
     4141 accgcggtcg cccagaggag cctggcggcg ctgggctccg gcgtcgtggt cgtcaccgac
     4201 gaccagctgg ccgcgctggg cggcacgccg aagctccggc tcgtcctgcc caactacccg
     4261 agggtcgagc gcttcgaggg ggccgcgccg gtggccgagc tcgccgccga cccgcgcctc
     4321 aagctcatct acgtcggcag cctctcgcgc gcgcgaggct gcacgatgat gctcgacgtc
     4381 atggagcagg tcgagccgga cgaggccgtc ctgtacctcg gcggcacctt caacgacccg
     4441 gcgctggagc tggaggtcgg cgagcggctg gccctgggcc tcgccgaccg cgtcaagctg
     4501 ctcggccgcg tcccgccgcc ggagctgccg cgctacctgg cggcggccga cgtcgtctgg
     4561 gtgccgtcgc agcccgaccg ccagtacagc caccccacca tccccaccaa gctgctcgaa
     4621 ggcatgacga tggggctcgc cgcgctggtc agcgacatgc cgggtcgcgg cgaactcgtg
     4681 cgcctcgaga agtgcgggct cgccgtcgag ccgggcgccg agggccacct gcgggggctt
     4741 cggcgcctcc tcatggaccg ccgggcgctg ccggagatgg gcgctcgcgg ccgccgcgcg
     4801 gtcgagcgcc gctacagctg ggaggcggtg cagggcgacc tggtcgactt ctacggctcg
     4861 ctctgcgccg ggctcccctg acggccgccc ggcgccgaga ccctacctcg gctcgccgaa
     4921 caggcacctc tcggcgttct tggcgaagaa cagctccctc accgccggct tgaccgcctc
     4981 ggtccacgcc tccacggcgc gtccgaactc caccagcggg tagtcggtgc cgaacaggca
     5041 gcggtccttg aggtaggagt tgaccgcctg gatggtcgcc tcgggcaggt acttcggcca
     5101 gagagcgctg aactcgagga agatgttcgg gtgcttctgc gccacggcca gggcgaggac
     5161 cccgaagccg ttgccggcgt gcgagatcac gagcttgagg ctcgggaagt tcatggcgat
     5221 ctcgtccatg tactgggggt cgcccagcca catgctctgg aagcggttgt agtgcaggct
     5281 gccgtggacg atcgccacca ggttgttgct ctcgcagtac gcgtagatgg ggtagagcac
     5341 gctggcgttg gccgggatgc cccagatgaa cgggctcacg ttgaccccga ggaagccggc
     5401 ctggcggcac cgctccagct cctccaggtt gcggcgcacg ccgttggatg gatggacgcc
     5461 ggcgatgtag tggaaggggt cgtcgtcgcc caaggagtcg cggacctcca tgatgtggtc
     5521 gttgtccgcg atggacccgc cgcacacgac gccgcgcgac acgccctcac tgcgcatcat
     5581 ggccacgaac tccgccatcc cgagcggcgt gagcctgggc cgcatgttgt acagctcgat
     5641 gtactgggag aagtgcggcg ccggatcgtc ggggttccag ggaccgagca tgtgcggtgt
     5701 gcgcaggcgc acgcggaagt cgacgacgtc catagtggat cctttcgtct ctcgggcggc
     5761 tagttcgatc gtggcagtcc ttccaggacg agcgaggtga tgtaccagtc agcgttgccc
     5821 gtgaccgcgg gctctccccg cagctcctcc gggatgtaga ggtagaactg gcgcggggcc
     5881 ggcctcttga acagcccgcc ccgcgtgaac agcgaggagt cgacgaaccc ggcgcggctc
     5941 aggcggcgcg gcaggagggc ggagcgccgc agcgccagac acttgacgcg agacagccgg
     6001 agctccttgc cccatcgggt cagcgccccg aagaggtggt cgaacaggtc ggcgcgggcg
     6061 ccgtccacga ggacgtcgac cacgtgcagg tcgccctcct ggacgaaggc gatggcgtac
     6121 ccgaggagcc cgtcgtcacc gtgcatggtc aggtacttgt gctccacgtg gggatcgtcg
     6181 ttcacccgcc accgcaggta cgcggcgctg cggtcgacgg tgatgccgta ccggcggcgg
     6241 ttccgctccc agaggtccgc gatcgcctcc agcggggcat ccgccgcgtc gtgcacggcc
     6301 accccgaacc ggcacgagcg cacgcgacgc gcgtggctgt acacctggag ggcaccggcc
     6361 agcagggagc tgagcacggg cgccagccgc ttcgcgccct tggccctcag gatggaaccg
     6421 atgtcctcgc ggagcacgct cgtcttcagc cagagcgtgt agttggccca gtcgtcgaac
     6481 gcgacgtagc cgagcttgct gcggacccgg ccgacggcgc ccgccgacac cgtgcccgcg
     6541 gtggtgaaga agaagctgta gcgctccttc tcccgctcga agcactctcg ctcgtgcgcg
     6601 gagtacaggc ccgtgccccg gtggtcgggg tggctcatgc agttctcggt cttgcccgcg
     6661 agcgccggac ggccccagac gctgagcggc gtggggatga ggctgtactg ggcgacgagt
     6721 tggtcgcctt cctcacggtc gaggtcgaag accaggttca tgctcccctg gccgtcccag
     6781 gtctcgagcc actcccaggc gaactcctcg ggcgtccggt ccctgcccgt gagtgacagg
     6841 tagagccggt tgatctgagc ctcgtcgccc ggctgatagg tacggctgac tgtacgcact
     6901 cggggagccc tgcctcgccc ggactgctcg tcgagcaggc cgtcgccacg ggcagtatcc
     6961 tccagcatcg cccgagcgca caaccgtcga cgggccggcc tcccgagcgt gtagtatgtg
     7021 gcgcatggac ggagctcagg atcgcgccga cgcgcgcgcc ggcatggtcg agggacggct
     7081 cctcaaggtc ctcgtcgtgt cgcacctcta tccgtggccc gaggccgaac gccacctctt
     7141 cgtccaccag caggtcctgg cgctcaagga gatcggcgtc gagtcgcgcg tgctgtcccc
     7201 gacgccgtgg gccccgcgtg ccctctggcg cagcgagcgc cagcggcggc gcggccgcaa
     7261 gccgcgcgaa gcggtccgcg acggcgtcgt cgccgagtac ccacgcgtcc tgcagccgcc
     7321 gcggcacctc ctcttcgatc gcctcggcga cctcgcctac cgcaccgtcc gccgcctgcc
     7381 ctcggtgcgg tcggccggct acgatctcgt ccacgcgcac caggcgctgc cggacggcgc
     7441 gctcgcgcag cgccttgccg ccgacctcgg cgtgccggtg gcgatcaccg tgcacggcgc
     7501 ggacgtctac cagcacctgc gcatgggcgg cgccgtcgag cggcgcgtgc ggcaggtgct
     7561 cggcggcgcc gacgcggtga tggcgaactc cagcgtcgtc gcccgcctgc tccgcgacgt
     7621 ggtcgcaccg gagaggctgt cggtcgtgct caacggcacc accggtctcg aagagcaggt
     7681 cgagccggcg tccgactacc tccccggcga
//
LOCUS       DASUMX010000282        26944 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_535097, whole genome shotgun sequence.
ACCESSION   DASUMX010000282 DASUMX010000000
VERSION     DASUMX010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 26944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26944
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535097"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            281..1591
                     /locus_tag="VFZ86_13360"
     CDS             281..1591
                     /locus_tag="VFZ86_13360"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HEX5643304.1"
                     /translation="MYFDPCAETMESEDLQELQLGRLKTVVTHAYSCSPAYRALCEEA
                     GVEPADIRSLDDIRRLPFIDKHTLRDNYPDKLRTCGLGGIIEVHSTSGTTGKPTPIWA
                     SRKDMDLWFERNARTMWMIGLRPGDLLQNMFGYGLPTSIGLQYGAQHAGIGVVPAGIG
                     RQELLIDLMVDLGVTAICTTPSYGLYLAEKALERGIDLSKDSRLRIGLFGAEPWPESG
                     RDRLAAALGVDVFNEYGMGEFLGPGMAAECPVKQGMHVWSDHIYVECIDPVTLEPVAD
                     GEPGELVWTSLTSDSTAMIRYRSHDISSLTYEPCACGRSHPRIGRITGRSDDAISVGG
                     LVVFPSQIEEVLLRFDEFGSNFCMVIDNVSNLDRLTVQVEVCGLEQMSDEAQAGLAKG
                     IVTGVKTSVGVTPKVELHAPYALPRDTTGQGKTACHRVDDRRTC"
     gene            1795..3471
                     /gene="hutU"
                     /locus_tag="VFZ86_13365"
     CDS             1795..3471
                     /gene="hutU"
                     /locus_tag="VFZ86_13365"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003987221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEX5643305.1"
                     /translation="MNTQTTAGPRPVRAPRGTEISCRGWQQEAALRMLMNNLDPEVAE
                     DPDQLIVYGGAGKAARNWESFDAIVRTLRRLKDDETLLVQSGKPVGVFETHDMAPRVL
                     IANANLVGDWANWDEFRRLDALGLTMYGQMTAGSWIYIGTQGILQGTYQTFAAAGQKH
                     YGADLAGRLVLTAGLGGMGGAQPLAVTMAGGVALCVEVDPHRIARRLETAYVDVSTDS
                     LDEALRLADEAMTARKPLSIALRGNAADVYPELVRRGVTPDLVTDQTSAHDTLNGYVP
                     NGMTFEEALRLRDDDPHEYVRRAVASIVVHVEAMVALQRAGAHTFDYGNNIRGEAKAA
                     GYADAFAFPGFVPAYIRPLFARGNGPFRWAALSGDPQDIAVTDAALLEAFPDNEMVTR
                     WIRMAGEKVHFQGLPCRICWLEYGERAKAGEIFNELVRTGKVKAPIVIGRDHLDSGSV
                     ASPYRETESMMDGSDAIADWPILNALLNTAAGASWVSFHHGGGVGIGKSLHAGMVVVA
                     DGTEMMGERLRRVLTTDPGTGVMRHADAGYPEAIEKAEESGIDLPMLGTD"
     gene            3488..3793
                     /locus_tag="VFZ86_13370"
     CDS             3488..3793
                     /locus_tag="VFZ86_13370"
                     /inference="COORDINATES: protein motif:HMM:NF024585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HEX5643306.1"
                     /translation="MNGSPVQLDLELCKACGVCIELCPDKVFDRDKLGYPMIARPEEC
                     SQCLLCELHCPDFAIEVRRRERKDTASAAAPTRDDLIAASSGAHAAGDGCTHDGEEA"
     gene            3795..5000
                     /locus_tag="VFZ86_13375"
     CDS             3795..5000
                     /locus_tag="VFZ86_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HEX5643307.1"
                     /translation="MTTTAAAAPASDAALAGRAVLLQGNEAVAEGAIAAGARFFAGYP
                     ITPSTEIAEVLSRRLPEVGGTFIQMEDEIASIAAVCGASLAGAKALTATSGPGFSLMQ
                     EMIGFATMAQIPCVVVDVMRGGPSTGLPTEPAQGDVMQARWGTHGEHSVIALVPNSVE
                     ECYTLTVQAFNLAEQYRTPVIILSDALIGHMREAVVLPEPGSLRIAERGMPDCEPDEY
                     DTIASTLDNILPRPPLGSPFKVHVTGLVYNRRSGTPDTSDPAAAADLGRYLREKIYHH
                     RDAIVRVEEVEMDGAEVAIFAYGSVARSALAVVREARAQGARVGMVRPITLWPFPTQA
                     VERMAEQVRTVIVPEMNIKQMSWEVQAAVNGRAKVVDHGRVDGKLITPEEILELVRPE
                     FPYHRWEDA"
     gene            4997..5890
                     /locus_tag="VFZ86_13380"
     CDS             4997..5890
                     /locus_tag="VFZ86_13380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008539286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX5643308.1"
                     /translation="MSALVDEQRLEASPTRDPKLPRIKPHTTSEIKAHLRSAKTLWCP
                     GCSNGTITRALVDAILGLQLDPEKVVVVAGIGCSGRVSAYLDYSTVHTAHGRALAVAT
                     GVKAADPSLHVIVAMGDGDSSAIGGNHFIHAARRNIDLTALVFNNSIYGMTGGQMSPT
                     THLGDKSTTSVYGNVEPAFDLCELAHAAGATYVARGTSWGYQQLASVIAGGIAHKGFA
                     MVEAMAICPVYYGRFNLTADPAQFLKSQKEHAVPADKFAEGETRSHEHYPVGLLHHSE
                     REEFITALGRIKELAREHGDG"
     gene            5883..6494
                     /locus_tag="VFZ86_13385"
     CDS             5883..6494
                     /locus_tag="VFZ86_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HEX5643309.1"
                     /translation="MADAARVDSAGGGRVEIRCSGSGGQGILLAAAIIAEAAAALGKR
                     VVQTQSYGPEARGGASKAEVIVSDEPIDYPEVRRPDVSLVLSQAAYEKYAVDTRPGGL
                     LIYDSGLVEVDPDDLSLVRCGLPFTQVATDELGKTVVTNIIAVGALARISEVLPAVAV
                     RNAVLNRVPAKFKELNGRAYDLGLRLGEGAVAMLADSSATGND"
     gene            6555..8153
                     /gene="hutH"
                     /locus_tag="VFZ86_13390"
     CDS             6555..8153
                     /gene="hutH"
                     /locus_tag="VFZ86_13390"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011553498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HEX5643310.1"
                     /translation="MTQLVIGDAPLTIDEVIAVARGGAGVSLGAAARGRIQAAYDVVA
                     DVIANEQVAYGVTTGFGLLATTHIPVDKVRELQENLVRSHAVGLGAPLSREVVRAAMT
                     IRLNTMARGHSGVRLSVAEHLAACINADIVPWVPSRGSLGASGDLAPSAHLVLAMMGE
                     GELLTEDGRREPSGPALRAAGLAPVSLEAKEGLSLLNGTQFMAAIGCMLVADGEALLD
                     SGDLIGAMSLEGLRASVGPFQERIQALRPIPGQLVTAENVRRATAGSAIMLSHLNCDK
                     VQDAYSLRCIPQVHGACRDALAWLRRVVGVEVDAVTDNPLIFPDSGEIISAGNFHGEP
                     LALALDLAAMSLAEIASISERRTFRMLTSSLSELPPFLTTDSGLNNGYMIAQYTAAAL
                     VSENKVLCHPASVDSIPSSGDQEDHVSMGMTAALKALDVLANAQRVLGIEAVCAAQAI
                     DLLAPLEPGSGTGAGRAVIREFAPTLARDRYLAPELEAAAEAVVAGRFAAIVRAAPAS
                     DVAAGLNDTSASHGSSEPSEGERT"
     gene            8150..9397
                     /gene="hutI"
                     /locus_tag="VFZ86_13395"
     CDS             8150..9397
                     /gene="hutI"
                     /locus_tag="VFZ86_13395"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HEX5643311.1"
                     /translation="MSVRCDVLVRGASEVVTMGPAGVIRRGAVAARDGRVVWTGPEAE
                     IASALEVPGDAVVVDVEGAAVIPGFVDAHAHLTWAGDRAAEYGARLGGATYLEIQAAG
                     GGINSTVRATRAATEEQLAALAARRLDSFLRHGTTTLEAKTGYGLTLEDERKQLAAAK
                     VPHPVRRVHTVLAAHLTPEEYAGRDDEYIALVCDEILPALVGQAEFVDVFCDEGAFTV
                     AQARCVLEAGRSLGYRLKIHAEELAHTGGTALAASLGAVSADHLIHITGEDIDALREA
                     GTVAVVLPGTSYTLRTVYAPARKLLAAGVTVALATDFNPGSCYSENLQMAISLACQED
                     RITPDEALRAATLGGAAALGLQHEVGSLERGKYCDLLVLDAATHQELPYHWGVNLVTG
                     VIAGGRVVVRERQVVHTADGGPA"
     gene            9394..10296
                     /gene="ftcD"
                     /locus_tag="VFZ86_13400"
     CDS             9394..10296
                     /gene="ftcD"
                     /locus_tag="VFZ86_13400"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HEX5643312.1"
                     /translation="MTLIECIPNFSEGRRQDVIDEIAAAVVGVDGVHLLDVQSDATHN
                     RCVVTFVGHQGPIAEAAFRAIETAAALIDLNRHEGAHPRFGAADVVPFVPLRAEDTPV
                     CIDTAQHLARRVATDLEIPAFLYEDAARIPEHKNLAVIRGKGFEEMREVIGSEPAKTP
                     DEGEARVHATAGAVAIGARFPLIAFNVNLDSGDLDLARRIAVEIRERDGGMKCVKAMG
                     LQLDSGTVQVSMNLTDYRVTSMADVVAAIGERASAAGVAIRESEVVGLLPQDALVRLA
                     QAALSAHGFTRAQVLEAQILDTLL"
     gene            10350..11510
                     /locus_tag="VFZ86_13405"
     CDS             10350..11510
                     /locus_tag="VFZ86_13405"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEX5643313.1"
                     /translation="MTAADTAGAGRAGRILITGLRLTPLFLTFKEPYHWAGRVDHGSP
                     VVLIEVETDAGIVGIGESVSSLAAEGTVAALRCVEPLFVGQPIHDVARLAHEARHLGS
                     FNHLPWYADFVLAGVEMALWDAIGKAAGQPVYRLLGGAVREEVDYFGFVQGDTTDELV
                     EHARALAAAGYGVIYLKVGRGDQADLANTAAVREVIGGRRLRVDPNCAWSVPDAIRMI
                     HALEPFGIDWVEQPTTLLSVTAMRQVREAVHVPIAADQAVLTPADVYEFVRQRAADAI
                     VLSPHEAGGLLAFKKAAAIAEAGGVPVCLHGQSVSGITDAAQHHLGLSTPNLTEGNQI
                     MHQLLVEDLVASPDLTPKQGKIGLIDAPGLGVDLDRDAVARAAECYQKGDLS"
     gene            11507..13141
                     /locus_tag="VFZ86_13410"
     CDS             11507..13141
                     /locus_tag="VFZ86_13410"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF015284.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEX5643314.1"
                     /translation="MTVAPYPEFFNDVFGPVMQPGSSSHTAGPCRLGYMAGCLLGEPV
                     ARMRIELDRGGSFAGTFGIMAEDRAMVAGVLGYLPDDARLFRSFELAEEAGVDIEFAF
                     TEITESRHPNAVKFVLTGQSGLMVTLVGDSTGGGMVETVTVDGFPYRTLGDAFVLFVQ
                     DPQEALSGEKLARLKAEVPVVIDAQTVRVDGRGVLYAFALPSEPDLAPLRAALGDVPV
                     LRLALLRPILSVVSRPDRKPQLFDTMTRWRELAADREAPLWEVAVHYEMDASGWTRTQ
                     VVDRMRELSLVMRRQTRAVYEEDVVAPDTEFKPDFAGRWIRHAETPARVSDGVTALTI
                     KYAYGAGAGIPGVENVPGPMGGGAGYIYAALSAVQEARGLSEDDLLRGLFVAAGIGAI
                     AYTRTEPTGEVLGCTGECGVCGAMAAAAIAEMVGGSPEAVEAAASLALQAFMGLPCDP
                     MPGGLSQPCRSRIMAATCTAHVFADLALAGHEAVLPLHEAIDVADRIGRALPPELLCT
                     SEGGCCLAPAAQARRAAYRRWFDESRPEDRPPGNLI"
     gene            13174..15447
                     /locus_tag="VFZ86_13415"
     CDS             13174..15447
                     /locus_tag="VFZ86_13415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014184604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX5643315.1"
                     /translation="MTEDLQYVGGNYPVHDVAQKVTGELVYGSDFSVPGMLHAKLILS
                     RIPHGMVKRIDASRAEALPGVVKVFSHENSPMDRFCRARIHPGQDLAQHDETLFSEHV
                     RFVGDRVAAVVATSRAAAEAAAALVDIEYEELPALLTVDEALASTDVLVHSTGNVAYE
                     HEIDRGERPDLEGAVIVSTSATTPRVHHAAIEPHVCIAQGHSTGGMTVWTTSQGVYAA
                     RTVIAELLRMEYHRVRVIKVPMGGSFGGKTEYIIEPVAAYLARETRRPVKLLLDREEC
                     MAATMVRPATQTTIRTACTPDGTLLDFEAASTLDSGAYASSTPDYSIHMCKKVTKVYR
                     VPHYRHQGRAVLTNTPVGGACRGWGAPEMAAATEIHMDRVAAVLRLDPVDLRLKNMVE
                     PFDTDLVTGLPLGDARVRECLERGAVAFDWTARRARPAGRGRLRRGVGVAAGAHKNGM
                     FGAFPEASNMSLKMNEDGTFELNASLHEVGCGIVTTMRVIVAEVLGVHPDLIAAGEAD
                     TATTPFDYGTYGSRVTYVVGACAREVAEKVKQRLLAAAAELLKEPVVHLFTREGAVHV
                     RHDADRAIAYRDIARLSHMRHVPSIAASTSYFAVSNPASYSVQFAEVEVDCATGLVAV
                     TDFLAVADVGRAINRGMVEAQYQGAVQMGIGYALYEHLDVDEKGRSGVDGFKNYHIVN
                     APDMPDVQVLLVEHEGDDGPFGAKSVGEIATVPTAPAIVNAVNNALGTELSDLPLIPE
                     RIIAALRGEEVAACSSC"
     gene            15432..16067
                     /locus_tag="VFZ86_13420"
     CDS             15432..16067
                     /locus_tag="VFZ86_13420"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX5643316.1"
                     /translation="MQLLLTVNGMVHEVEARPTARLLDVLRDQLGLTGTKEGCAEGEC
                     GACTVIVDGKAVNSCVMLAVQARGKNILTVEGLAPDGELDLLQQKFVEYGAVQCGYCT
                     PGMLMSAKALLMGNPVPSEQDIRIALAGNLCRCTGYSAIVAAVKAASGQEAVPLMELP
                     GDAEELAAEVTTDPETLAAACEVAQADAGGPLGAPCDSDGPPPASEPEGGE"
     gene            16069..16956
                     /locus_tag="VFZ86_13425"
     CDS             16069..16956
                     /locus_tag="VFZ86_13425"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEX5643317.1"
                     /translation="MASTQVLTPTSVEELVTALGQATPRTRLLAGGTDLVRAIRRPGG
                     EPDLLVDLTGVTELASVRLQGGSLRVGALATFTKLQWDPLVREHALCLSLAAAQVGSA
                     QIRNAGTIGGNIANASPCADGATALTALGAEVTTVDGAGATQTRPIGDVLLGPNRTGL
                     RHDEAIIEFTFPALGAAHRSAFAKIGSRTAVSVARLSAALVVRFDAPSGAITDVRAAL
                     GAVGAVAFRAPELERALEGRLADEKSARIFAEGCVAAVRKSIPGRYSLPYKQHAAVGI
                     AYDAWNMLALSAPFEPAWD"
     gene            complement(17133..18788)
                     /locus_tag="VFZ86_13430"
     CDS             complement(17133..18788)
                     /locus_tag="VFZ86_13430"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5643318.1"
                     /translation="MQDDRHAKEYADHLIQEVRTGRMTRRQLLVRASVFGFSLTAAGS
                     LLAACGGDSTTTSEGSPSASAAPQKGGSIKVTALTPLSALDPVTMYQTGDISTVEQVC
                     EYLIWINNDLTLRPVLAESWSPDPTGAVWTFKLRQGVSFNDGSPLAADDVVATFDRLV
                     DPDSGSGALSALKGILSQGGTQKIDDYTVQFNLDAAFADFPYLVSSGNYNSVILHRSF
                     AGDFTKKPVGTGPFMLTDYQDKQSATLKRNPNYWQKGLPYLDEVGFVYIDDAQAQALQ
                     LQSGAVDLQAQTAFQGSQALFANPDLNVITTHSTAIREVAMRVDKEPYSDKNVRQAVA
                     YCLDRQAIMASLYDGKGTQGNDQIFSPLYPTSPSFADRAQDYDKAKQLLSDAGYAGGV
                     KLTLTTASSYLDLAQYAQIIAEQCKPAGIEVKLNTVSATEFYGGDSSTTPWLNADMTI
                     VEWSPRPVAAQYIQAMLTKASVWNSSHWANSEFDTLFKQHSATVDEASRSEIATKMGT
                     IQQDETPVILAFWMDSLRATTKRVHNVQGLEAYLDLTNAYVTA"
     gene            complement(18926..20320)
                     /locus_tag="VFZ86_13435"
     CDS             complement(18926..20320)
                     /locus_tag="VFZ86_13435"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HEX5643319.1"
                     /translation="MGKPAIRVWDTEECELVHEATLRVLSECGVEVMWEPALRRFAEA
                     GAKVDGSRVRLSSELVSRALASAPRTWSVRSRGRDEVLELKDGPTYFGSGSDCLYVTD
                     PDTGQRRRAVVDDIEALAGLCEKLDNIDFVMSMGFPENVPPETEVLHQQVALLKGTRK
                     PLLIVPRDGHTLATMKAMAEACGGGDSLIVYGMPSPPLMHDEFALSKIETCAELMIPL
                     VYAPAPTAGSTAPASIAAVVVTGNAEVLSGLVLHQLVNPGAPFVYGAGCDTLDMRTMV
                     NPYVSPEWSMGCQAGCDLARFYSLPSFSYGGYSDAKMLDEQWSAETAMTAVMGALSRG
                     TLLHDVGYLEMGMQTSFESIVLGDELAGFAKACLRDIAVDDEALAVDEIISVGPGGNH
                     LPRRYTRTHYREFWTPTLLDKAPHDRWQNNGATSLKQRVQQRVAELRAEPPRFALDDQ
                     ALGEIERLLRRAAG"
     gene            20581..21447
                     /locus_tag="VFZ86_13440"
     CDS             20581..21447
                     /locus_tag="VFZ86_13440"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HEX5643320.1"
                     /translation="MRGEDESVPGVKPVLDLGPLRPRSSSMLVADSLRRLIALGALSP
                     GERLPSERELAETLRVGRDSIRQAIRTLNDEGLVETRLGRSGGTVVLDRPVQPKRLSE
                     EILASHREIRASYEFRLAIEPAAASMAARRATTEDVARITAIADEQAWSFRSWRSIDS
                     RFHAAVAEASGNGLLLDAVQRTRTAFFSWYDAIYSRLPWDSLPIQDRDFAYLHRPIAE
                     AIADGDPERAGLLMKEALEWSEQDLEDLLDGVVAHASARRENVPRSMEPDPAGQTAPG
                     AADARRAADRDG"
     gene            complement(21490..22497)
                     /locus_tag="VFZ86_13445"
     CDS             complement(21490..22497)
                     /locus_tag="VFZ86_13445"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEX5643321.1"
                     /translation="MSVLIADQDLVTELLPMEDAIGVMRRALTLLAEGEVMMPLRSML
                     HLPKGGAVMGLMPSYLGGLEAVGVKVIAEFPANFGSEYDTHQGVVLFFDTQRGLLRAM
                     VDATAVTAIRTAAVSGLVTDLLANPDAGDLAIIGAGTQAHTHLQAMAAVRPVHRVRVY
                     SVPAESAAMFADRESRRTGIEIEVAASAEQAVEGADLICTVTTSHEPVVHGDWVSPGA
                     HINAVGAYNPATRELDSALVAKSRLYADRRESLLNEAGEFLIPKGEGLFGDEHIVGEI
                     GEVLTGKAPVRTSPQEITLFKSLGIAIEDLAAAHYVYEAAKARGLGQWVEIGGRHFGA
                     G"
     gene            complement(22494..23612)
                     /locus_tag="VFZ86_13450"
     CDS             complement(22494..23612)
                     /locus_tag="VFZ86_13450"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEX5643322.1"
                     /translation="MKITDISTTPLWLPFKEPYHWAGRIDYGAAVVLVEVFTDEGVVG
                     VGESTAGIPAHSTVAALKSVAHLLVGEPAFDVERLVTKARYLGSFNHTPRAANLALAG
                     FEMALWDVLGKAAGWPVFRLLGGSVRDDVDYFGFVQGDTTEELVEDARDLAAAGYSVI
                     YLKVGRGAEADLRNTAAVREAIGARRLRLDANGAWGVTEAIHMIGRLAPFEPEWIEQP
                     TPPTSVAAMRQVKDGVTVPIAADQAAFTPADVYEICRQRAADAIVLSAHEAGGLHAFA
                     KAAAIAEAAGLPVCLHGQNVSSISDAAQHHLGLSTSNLSEGNQIMHQLIAEDLVVSPD
                     LTPICGKIGPLETPGLGVELDRDAIARAAERHAKEKWL"
     gene            complement(23892..26063)
                     /locus_tag="VFZ86_13455"
     CDS             complement(23892..26063)
                     /locus_tag="VFZ86_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008877841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5643323.1"
                     /translation="MSSVENQQPAMTDAAALDVSDLEVTFHRRGRRLPVLKGVTLKIE
                     RGEAYGLVGESGCGKTTLAMAAMRYLAANGTLDNGRVLVDGQDVSELSEEALRKWRGG
                     KVSMVYQDPTQALSPAMRIGDQLAEVFHYHEGLDKKAALEKARESLRRVAIPDPDGTL
                     KRYPFELSGGQQQRVVIAMALAVNPQLLILDEPTTGLDATVEAEILDLIEELRGRINA
                     AILLITHNLGLVVRLCERVGVLYAGRLVEEGPAKEVFTDPRHPYTMGLLRCVPRFGMN
                     KTDAALQTIPGTLPALGDPLEGCVYSARCPMAKPVCTRREPDFFAWPSDAPERGRSEA
                     EAMPPDPAAYSPHHSEPLGDSDRHARCYFCADTPGMPHTTPVLATSLEERGGESDVLA
                     IDRVVRTFKDGRNQLTAVADVSLSLKKGETFGLVGESGSGKTTLAKCVTGLLPPDSGT
                     ITFEGGVLKAKAGRRSQDALRAIQMVFQNPDSTLNPAWSTRQILTRAVRKLGGAKEGS
                     VRAKVDKLSADVRVEPRFLFQRPTELSGGQKQRVAIARAFAGDPHLVICDEPASALDV
                     SVQASILNLLVELQTKERTSYVFISHDLAVVRYISDRIGVMYLADLIEVGAAADVFSA
                     PHHPYTEALLSSIPRLDFEKQQDRIPLRGSMPSLTDPPSGCRFHTRCHRFLGEICVEQ
                     EPAAMDAGNGHTYKCHIPPDELRALQMHDRPAPQPEPAPAD"
     gene            complement(26060..26866)
                     /locus_tag="VFZ86_13460"
     CDS             complement(26060..26866)
                     /locus_tag="VFZ86_13460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5643324.1"
                     /translation="MVKALLRSPTFMIGLVIVLFWVFMALFSTSITQSFTAQDAASSL
                     QAPSAAHWFGTDDLGRDVFARTMAGARSVLIIAPLATLLALLWGGIIGLISGFYRGIT
                     DEIIMRAIDVLLALPIIITSILILSLFGKGTAIIIIAIGALFTPVVSRTVRAAVIGER
                     EREYVMAARLRGERSVFVMSREILPNITQPIIVEGTIRLGYAVFTAATLSFLGFGLVP
                     PSPDWGLTIANERIFLQIAPWTVMFPAAALASLVVAVNLITDGLGRTFSK"
BASE COUNT         4172 a         9386 c         9395 g         3991 t
ORIGIN      
        1 aggtcggtga cctcaaggtg gccggcatcg tgctggtcgg cgcgctcagc atgcacctgc
       61 cgttcgacgt cgagacgtgg caggcgagca tcacgcgcaa cgttccgccg aagtggaggg
      121 acctgaacta cgcggcgttc gcggccggac gggaggcggg ggccggatga acagccgccg
      181 taggcatgct gtcgagcggg gcgccgcagg cacaccgctc tgcgccacgt gcccgggcgg
      241 cgcggccgca ggctcgcacg ccgcccacga cgaggaccgc gtgtatttcg acccgtgcgc
      301 cgagacgatg gagtcggagg acctgcagga gctacagctc ggccggctca agaccgtggt
      361 cacgcacgca tactcctgca gcccggccta cagggcgctc tgcgaggagg ccggggtcga
      421 gccggcggac atccggtccc tcgacgacat ccgccggctc cccttcatcg acaagcacac
      481 cctgcgagac aactacccgg acaaactccg cacctgcggc ctcggcggca tcatcgaggt
      541 ccacagcacc tccggcacca ccggcaagcc cacgcccatc tgggcctccc gcaaggacat
      601 ggacctctgg ttcgagcgca acgcgcggac catgtggatg atcggcctgc gccccggcga
      661 ccttctgcag aacatgttcg gctacggcct gccgaccagc atcggcctgc agtacggcgc
      721 ccagcacgcc ggcatcgggg tcgtgcccgc cggcatcggt cgtcaggagt tgctcatcga
      781 cctgatggtc gacctcggcg tgaccgccat ctgcaccacg ccgtcctacg gcctgtacct
      841 cgccgagaag gcgctcgagc gcggcatcga cctgagcaaa gacagcaggc tgcgtatcgg
      901 cctcttcggc gccgagccgt ggccggagag cggccgcgac cgcctcgccg ctgccttggg
      961 cgtcgacgtc ttcaacgagt acggcatggg cgagttcctg ggaccgggca tggcggcgga
     1021 gtgcccggtc aagcagggca tgcacgtgtg gtcggaccac atctacgtgg agtgcatcga
     1081 cccggtcacg cttgagccgg tcgccgacgg cgagcccggc gagctcgtct ggacgtcgct
     1141 caccagcgac tcgacggcca tgatccgcta ccgcagccac gacatcagct cgctcacgta
     1201 cgagccgtgc gcgtgcggcc gcagccaccc gcgcatcggc cgcatcaccg ggcggagcga
     1261 cgacgccatc tccgtcggag gcctcgtcgt cttccccagc cagatagagg aggtactgct
     1321 gcggttcgac gagttcggca gcaacttctg catggtcatc gacaacgtga gcaacctgga
     1381 ccggctgacg gtgcaggtcg aggtctgcgg cctggagcag atgagcgacg aggcccaggc
     1441 cggtctcgcc aagggcatcg tgacgggcgt caagacgagc gtgggcgtga cccccaaggt
     1501 cgaattgcac gccccctacg cgctgcctcg cgacacgacc ggccagggca agaccgcctg
     1561 ccaccgcgtc gacgaccggc gtacctgctg aggtcgccgc cggccagacg ccttcaacac
     1621 ctctcctcga ttccagcatc gcggcccatt cccgtattcg ggaacgaaat cgcgtccatg
     1681 tttctggaca agacgcgcga ctgcttgaag cgatccctgc ttcggcgcag ggtggagcca
     1741 gaacgcgcgg gagacggcgc gccctgacaa ccacccacgt agcgaggcgg catcgtgaac
     1801 acacagacca cggcgggacc gcggccggta cgcgctccgc gcggcaccga gatcagctgc
     1861 agaggctggc agcaggaggc cgccctccgc atgctgatga acaacctcga tccggaggtc
     1921 gccgaggacc cggatcagct catcgtgtac ggcggggccg gcaaggcggc gcgcaactgg
     1981 gagagcttcg acgccatcgt ccgcaccctg cgccgcctca aggacgacga gaccctgctc
     2041 gtgcagagcg gcaagcctgt cggcgtgttc gagacccacg acatggcgcc gcgcgtgctc
     2101 atcgccaacg ccaacctggt cggcgactgg gccaactggg acgagttccg ccggctggac
     2161 gcgctggggc tcaccatgta cgggcagatg accgccggct cgtggatcta catcgggacg
     2221 cagggcatcc tgcagggcac ctaccagacc ttcgctgccg cggggcagaa gcactacggc
     2281 gccgacctgg ccggcaggct ggtgctgacg gccggcctcg gcggcatggg cggggcgcag
     2341 ccgctcgccg tcaccatggc cggtggcgtc gcgctgtgcg tggaggtcga tccgcaccgc
     2401 atcgcgcggc gcctcgagac cgcctacgtg gacgtgtcga cggactcgct ggacgaggcg
     2461 ctcaggctgg cggacgaggc gatgacggcg cgcaagccgc tctccatcgc ccttcgcggc
     2521 aatgccgccg acgtctaccc cgagctcgtc cgccgcggcg tcacccccga tctggtcacc
     2581 gaccagacct cggcgcacga cacgctcaac ggctacgtgc ccaacggcat gacgttcgag
     2641 gaggccttgc gcctccgcga cgacgacccg cacgagtacg tgcgccgcgc cgtcgcgagc
     2701 atcgtcgtcc acgtcgaggc gatggtcgct ctgcagaggg ccggcgccca caccttcgac
     2761 tacggcaaca acatccgcgg cgaggcgaag gcggccggct acgccgacgc cttcgccttc
     2821 cccggcttcg tgcccgccta catacggccg ctcttcgccc gcggcaacgg gccgttccgc
     2881 tgggcggcgc tcagcggcga cccgcaggac atcgccgtca ccgacgccgc cctgctcgag
     2941 gccttcccgg acaacgagat ggtcacgagg tggatccgca tggcaggcga gaaggtgcac
     3001 ttccaggggt tgccttgccg catctgctgg ctcgagtacg gcgagcgcgc caaggccggc
     3061 gagatcttca acgagctggt gcgcaccggc aaggtcaagg cgcccatcgt catcggccgc
     3121 gaccacctcg actccggctc ggtcgccagc ccgtaccgcg agaccgagag catgatggac
     3181 ggcagcgacg ccatcgccga ctggcccatc ctcaacgcgc tgctcaacac cgcggccggc
     3241 gccagctggg tgagcttcca ccacggcggc ggcgtcggca tcggcaagag cctgcacgcc
     3301 ggcatggtcg tggtcgccga cggcaccgag atgatgggcg agcgcctgcg ccgcgtgctg
     3361 accaccgacc ccggcaccgg cgtgatgcgt cacgccgacg cagggtatcc cgaggccatc
     3421 gagaaggccg aggaatctgg catcgacctt ccgatgctgg ggacggactg aggggctggc
     3481 ggaagtcatg aacggttcac cggtccagct ggatctcgag ctctgcaagg cctgcggcgt
     3541 ctgcatcgag ctctgcccgg acaaggtctt cgaccgcgac aagctcggct acccaatgat
     3601 cgcgcggccg gaagagtgca gccagtgcct gctctgcgag ctccactgcc ccgacttcgc
     3661 catcgaggtc cggcggcgcg agcgcaagga caccgcgagc gccgccgcgc cgacgcgcga
     3721 cgacctcatc gcggcctcga gcggcgcgca tgcggccggc gacgggtgca cccacgacgg
     3781 cgaggaggcc tgagatgacc accactgcgg cggcagctcc cgcctcggac gcggcgcttg
     3841 cgggccgcgc cgtcctgctg cagggcaacg aggcggtcgc cgagggcgct atcgccgccg
     3901 gcgcccgctt cttcgccggc tacccgatca ccccgtccac ggagatcgcc gaggtgctct
     3961 cccgccggct cccggaggtg ggcggcacgt tcatccagat ggaggacgag attgccagca
     4021 tcgccgccgt ctgcggcgcg tcgctggccg gcgccaaggc gctcaccgcc acctccggac
     4081 ccggcttcag cctgatgcag gagatgatcg gcttcgccac catggcgcag atcccctgcg
     4141 tggtggtgga cgtcatgcgc ggcgggccga gcaccggcct gccgaccgag ccggcgcagg
     4201 gcgatgtgat gcaggcccgt tggggcacgc acggcgagca ctctgtgatc gcgctggtcc
     4261 ccaacagcgt cgaggagtgc tacacgctca ccgtccaggc gttcaacctc gccgagcagt
     4321 accgcacccc ggtcatcatc ctcagcgacg cgctgatcgg ccacatgcgt gaggccgtcg
     4381 tcttgccgga gcccggcagc ctgcgcatcg cagagcgcgg catgccggac tgcgagccgg
     4441 acgagtacga cacgatcgcc agcacgctgg acaacatcct gccgcgaccg cctctcggct
     4501 cgccattcaa ggtccatgtc accggcctcg tctacaaccg ccgcagcggc acgccggaca
     4561 cgagcgaccc tgccgccgcc gccgacctcg ggcggtacct gcgcgagaag atctaccacc
     4621 accgcgacgc gatcgtccgt gtggaggagg tcgagatgga cggcgccgag gtcgcgatct
     4681 tcgcctacgg ctccgtcgcc cgctcggccc tggcggtggt ccgcgaggcc cgcgcccagg
     4741 gcgctcgcgt cggcatggtg cggcccatca ccctgtggcc gttcccgacg caggcagtgg
     4801 agcgcatggc cgagcaggtg cgcacggtca tcgtcccgga gatgaacatc aagcagatga
     4861 gctgggaggt ccaggccgcg gtcaacgggc gcgccaaggt ggtggaccac gggcgtgtgg
     4921 acggcaagct catcacgccc gaggagatcc tcgagctcgt ccgacccgag ttcccctacc
     4981 accgctggga ggacgcatga gcgccctggt cgacgagcaa cggctcgagg cgtctccgac
     5041 ccgcgacccc aaactgccgc ggatcaagcc gcacaccacg tcggagatca aggcccacct
     5101 gcgctcggcc aagacgctgt ggtgcccggg ctgctccaac ggcaccatca cccgggcgct
     5161 cgtcgacgcc atcctcggcc tccagctgga cccggagaag gtcgtcgtgg tcgccggcat
     5221 cggctgcagc ggccgcgtca gcgcctacct cgactactcg acggtgcata cggcacacgg
     5281 ccgggcgctc gccgtggcga cgggcgtcaa ggccgcggac ccgtcgctgc atgtgatcgt
     5341 cgccatgggc gacggcgaca gctccgccat cggcggcaac cacttcatcc acgcggcgcg
     5401 gcgcaacatc gacctcacgg cgctggtctt caacaactcc atctacggca tgaccggcgg
     5461 gcagatgagc cccaccacgc acctcggcga caagtcgacg acgagcgtgt acggcaacgt
     5521 cgagccggcg ttcgacctct gcgagctcgc ccatgccgcg ggagccacct acgtcgcccg
     5581 cggcacctct tggggctacc agcagctggc gagcgtcatc gccggcggca tcgcccacaa
     5641 gggcttcgcc atggtcgagg ccatggcgat ctgcccggtc tactacggcc gcttcaatct
     5701 caccgccgac cccgctcagt ttctcaagag ccagaaggag cacgcagtgc cggccgacaa
     5761 gttcgccgag ggcgagacgc gcagccacga gcactacccg gtcgggctgc tccaccacag
     5821 cgagcgcgag gagttcatca ccgcgctggg tcgcatcaag gagctggctc gggagcacgg
     5881 cgatggctga cgcagcacgc gtcgacagcg ccggcggggg ccgcgtcgag atccgctgct
     5941 cggggtccgg tggccagggc atcctgctgg ccgcggccat catcgcggag gcggccgcgg
     6001 ccctcggcaa gcgggtcgtg cagacacaga gctacggacc cgaggcccgc ggcggagcct
     6061 ccaaggcaga ggtcatcgtc tccgacgagc cgatcgacta ccccgaggtg cggcgtccgg
     6121 atgtcagcct cgtcctctcg caggcagcgt acgagaagta tgcggtcgac acccggcccg
     6181 gaggtctgct gatctacgac tccgggctcg tcgaagtgga ccccgacgat ctgtccctgg
     6241 tccgctgcgg cctgccgttc acgcaggtcg ccaccgacga gctgggcaag accgtcgtca
     6301 ccaacatcat cgcggtcggc gcgctggcac ggatcagcga ggtgctgccg gcggtagcgg
     6361 tgcgcaacgc cgtcctgaac cgcgtcccgg cgaagttcaa ggagctgaac gggcgggcgt
     6421 acgatctcgg gctgcggctg ggcgaagggg cggtcgcgat gctggcggac tcgtccgcga
     6481 ccgggaacga ctagactgac tcgcggcgag ctcgcccgcc tgcccggaga ggccatcacg
     6541 aggagaagga ccacatgacg cagctcgtca tcggcgacgc gccgctgacc atcgacgagg
     6601 tcatcgccgt cgcgcgcggc ggggctgggg tgagcctcgg cgcggctgcg cgcgggcgca
     6661 tccaggcggc atacgacgtc gtggccgacg tcatcgcgaa cgaacaggtc gcgtacggcg
     6721 tcaccaccgg cttcggcttg ctcgcgacca cgcacatccc cgtggacaag gtccgcgagc
     6781 tgcaggagaa cctcgtgcgc agccacgcgg tcggcctcgg cgcgccgctc tcgcgcgagg
     6841 tcgtgcgcgc ggccatgacc atccgcctca acacgatggc gcgcggtcac tccggcgtca
     6901 ggctctcagt ggccgagcac ctggccgcgt gcatcaacgc cgacatcgtc ccgtgggtgc
     6961 cgtcgcgggg gtcactcggc gcctccggcg acctcgcccc gtccgcgcac ctcgtcctgg
     7021 cgatgatggg cgagggcgag ttgctcacgg aggacgggag gcgcgagccg agcgggcccg
     7081 ccctgcgggc cgccgggctc gcgcccgtct ctctcgaagc aaaggagggc ctcagcctcc
     7141 tcaacggcac tcagttcatg gccgccatcg gctgcatgct ggtcgccgac ggggaggcgc
     7201 tccttgattc gggcgatctg atcggtgcga tgagcctcga gggcctgcgc gcctccgtcg
     7261 gcccgttcca ggagcgcatc caggcgctgc gccccatccc cggccagctg gtcaccgcgg
     7321 agaacgtccg ccgcgcgacc gccgggagcg cgatcatgct cagccacctg aactgcgaca
     7381 aggtgcagga cgcctactca ctgcgctgca tcccgcaggt gcacggcgcc tgccgcgatg
     7441 cgctcgcctg gttgcggcgc gtcgtcggtg tcgaggtcga cgctgtcacg gacaacccgc
     7501 tcatcttccc ggactcgggc gagatcatct cggcgggcaa cttccacggc gagccgctcg
     7561 cgctggctct cgacctcgcg gcgatgagcc tcgccgagat cgcctccatc agcgagcgcc
     7621 ggaccttccg catgctcacc agctcgctca gcgagcttcc gcccttcctc acgaccgaca
     7681 gcgggctcaa caacggctac atgatcgccc agtacaccgc ggccgcgctg gtgagcgaga
     7741 acaaggtgct ctgccacccg gccagcgtgg actccatccc cagctcgggg gatcaggagg
     7801 accacgtgag catggggatg acggcggcac tcaaggcgct ggacgtgctt gccaacgcgc
     7861 agagggtgct cggcatcgag gccgtctgcg ccgcgcaggc gatcgacctg ctggcgcctc
     7921 tggagcccgg ctccggtact ggggccgggc gggccgtgat ccgcgagttc gcgccgacgc
     7981 tggcgcgcga ccgctatctg gcgccggagc tcgaggcggc ggccgaggcg gtcgtggccg
     8041 gacggttcgc ggccatcgtt cgggcggccc cggcctccga tgtcgcagcc gggttgaacg
     8101 atacgtccgc ttcgcacgga tcatccgaac cgagtgaagg agaacgcacg tgagtgtgcg
     8161 ctgtgatgtg ctcgtccgcg gcgcgagcga ggtcgtgacc atgggtccgg ccggcgtgat
     8221 acggcgcggc gccgtcgccg ctcgcgacgg ccgcgtcgtc tggaccggcc cggaggcgga
     8281 gatcgcgagc gctctcgagg tgcccggcga tgcggtcgtc gtggacgtcg agggcgccgc
     8341 cgtcatcccc ggcttcgtcg acgcgcacgc gcacctcacg tgggcgggcg accgggcggc
     8401 cgagtacggc gcccggctcg gtggcgccac ctacctggag atccaggcgg ccggtggcgg
     8461 catcaacagc acggtgcgcg cgacgcgggc ggcgacggag gagcagctcg ccgctctcgc
     8521 cgcgcgccgc ctcgactcct tcctgcggca cggcaccacg accctcgagg ccaagaccgg
     8581 ctacgggctc accctcgagg acgagcgcaa gcagctcgcc gcggccaagg tgccccaccc
     8641 ggtgcgccgc gtgcacaccg tcctcgcggc gcacctgacg cctgaggagt acgccggccg
     8701 cgacgacgag tacatcgccc tggtctgcga cgagatcctg ccggcgctcg tgggccaggc
     8761 ggagttcgtc gacgtcttct gcgacgaagg cgcgttcacc gtcgcgcagg cgcgatgcgt
     8821 gctcgaggcg gggcgctctc tcgggtaccg gctcaagatc cacgccgagg agctggcgca
     8881 caccggtggg acggcgctgg ccgcttcgct gggcgccgtc tccgcggacc acctcatcca
     8941 catcaccggc gaggacatcg acgcgctcag ggaggcaggc accgtggccg tcgtgcttcc
     9001 gggcacgagt tacacgctgc gcacggtgta cgcgccggcg cggaagctgc tggccgccgg
     9061 tgtcaccgtg gcgctcgcca ccgacttcaa ccccggcagc tgctacagcg agaacctcca
     9121 gatggccatc tcgctggcct gccaggagga ccggatcacc ccggacgagg ccctccgcgc
     9181 cgccacgctc ggcggcgccg ctgcgctcgg cctgcagcac gaggtcggca gcctcgagcg
     9241 gggcaagtac tgcgaccttc tcgtcctgga cgcagcgacg caccaggaac tgccctacca
     9301 ctggggcgtc aacctcgtga ccggcgtgat cgccggcggc cgggtcgtcg ttcgcgaacg
     9361 gcaggtggtt catacggcag acggaggtcc cgcgtgaccc tcatcgaatg catccccaat
     9421 ttcagcgagg gtcgccgtca ggacgtgatc gacgagatcg cggcggcggt ggtcggcgtc
     9481 gacggcgtcc atctgctcga cgtgcagagc gacgcgacgc acaaccgctg cgtcgtcacc
     9541 ttcgtcggcc accagggccc catcgcggag gcggcgttcc gcgccatcga gaccgccgcc
     9601 gccctcatcg acctcaaccg tcacgagggc gcccatccgc gtttcggagc ggccgacgtc
     9661 gtgccgttcg tcccgcttcg cgcggaggac accccggtct gcatcgatac tgcgcagcac
     9721 cttgcccgaa gagtcgcgac cgacctcgag atccccgcgt tcctctacga ggacgcggct
     9781 cgcatccccg agcacaagaa cctcgccgtc atccgcggca agggcttcga ggagatgcgc
     9841 gaggtgatcg gcagtgagcc cgccaagacg ccggacgagg gagaggcgcg cgtgcacgcg
     9901 accgccgggg cggtcgcgat cggcgcgcgc ttcccgctca tcgccttcaa cgtgaacctc
     9961 gactccggcg atctggatct tgcgaggcgc atcgccgtcg agatccgcga gcgcgacggc
    10021 ggcatgaagt gcgtcaaggc catggggctg cagctcgaca gcggcaccgt gcaggtctcg
    10081 atgaacctca ccgactaccg ggtgacgtcg atggccgacg tggtcgccgc catcggcgag
    10141 cgcgcctccg ccgccggcgt cgcgatccgc gagagcgaag tcgtcggact tctgccgcag
    10201 gacgcgctcg tgaggctggc gcaggcggcg ctctccgccc acggcttcac gcgcgcccag
    10261 gtgctcgagg cccagatcct ggacacgctg ctgtgagtcc acgcagacaa ggtaccgagc
    10321 cgggcgctga agggggaccg ctctccaccg tgaccgccgc ggacaccgcc ggcgcaggtc
    10381 gcgccgggcg catcctcatc acgggcctcc gcctcacgcc gctcttcctg acgttcaagg
    10441 agccgtacca ctgggccggc cgcgtggacc acggctcccc cgtggtgctc atcgaggtgg
    10501 agaccgacgc cggtatcgtc gggatcgggg agtccgtctc gtcgctcgcc gccgagggga
    10561 ccgtcgcggc gctccgttgc gtcgagccgc tgttcgtggg tcagccgatc cacgacgtgg
    10621 cgcggctggc gcacgaggcg cggcacctcg gcagtttcaa tcacctgccc tggtacgccg
    10681 acttcgtcct ggccggagtc gagatggcgc tgtgggacgc gatcggcaag gccgccggcc
    10741 agccggtgta ccggcttctc ggcggcgccg tgcgcgagga ggtcgactac ttcggcttcg
    10801 tgcagggcga cacgacggac gagctggtgg agcacgcccg tgcacttgcg gccgcgggct
    10861 acggcgtcat ctacctcaag gtaggccgcg gcgaccaggc cgacctcgcg aacacggcgg
    10921 cagtgcgcga ggtcatcggc ggccgccgtc tgcgcgtgga ccccaactgt gcctggagcg
    10981 tccccgacgc catccgcatg atccatgcgc tggagccctt cggcatcgac tgggtcgagc
    11041 agccgacgac gctgctcagc gtgactgcca tgcgccaggt ccgcgaggcc gtgcacgttc
    11101 ccatcgccgc cgaccaggcc gtgctcaccc cggcggacgt ctacgagttc gtgcgccagc
    11161 gtgcggcgga cgcgatcgtc ctctcgccgc acgaggccgg cggcctgctc gctttcaaga
    11221 aggccgccgc gatcgccgag gccggcggcg tccccgtctg cctgcacggc cagagcgtct
    11281 ccggcatcac cgacgccgcc cagcaccacc tcggcctcag cacacccaac ctcaccgagg
    11341 gcaaccagat catgcaccag ctgctcgtcg aggaccttgt ggcgtcacct gatctcaccc
    11401 cgaagcaggg caagatcggc ctcatcgacg cgccgggact cggcgtcgat ctggaccgcg
    11461 acgcggtcgc ccgcgccgcc gagtgctacc agaagggaga cctctcgtga cggtcgcacc
    11521 gtatcccgag ttcttcaacg acgtcttcgg gccggtcatg cagcccggct ccagttcgca
    11581 caccgccggg ccgtgccggc tcgggtacat ggcaggctgc ctgctcggcg agccggtcgc
    11641 gaggatgcgc atcgagctcg acaggggtgg ctcgttcgcc gggaccttcg ggatcatggc
    11701 cgaggaccgg gcgatggtcg ccggcgtcct cggctacctg cccgacgacg cccggctgtt
    11761 ccgcagcttc gagctcgccg aagaggcggg cgtcgacatc gagttcgcct tcacggagat
    11821 caccgagagc aggcatccca acgccgtcaa gttcgtcctg acgggccaga gcggcctcat
    11881 ggtgacgctg gtcggcgact cgacgggcgg cggcatggtc gagacggtca cagtcgacgg
    11941 cttcccgtac cggacgctcg gcgacgcctt cgtgctgttc gtgcaggacc cgcaggaggc
    12001 cctgagcggc gagaagctcg cacggctcaa ggccgaggtc cccgtcgtga tcgatgcgca
    12061 gaccgtgcgc gtcgacggcc gcggagtcct ttacgccttc gcgcttccct ccgagccgga
    12121 cctcgcgccg ttgcgcgcag cgctcggcga cgtgcccgtc ctccggctgg cgctgctgcg
    12181 gccgatcctg tccgtcgtca gccggccgga ccgcaagccg cagctcttcg acaccatgac
    12241 gcgctggcgc gagctggcgg ccgaccgcga ggcgccgctc tgggaggtcg ccgtccacta
    12301 cgagatggac gcgtccggct ggactcgcac ccaggtcgtg gaccgcatgc gcgagctctc
    12361 ccttgtgatg cgtcgccaga cgcgggccgt gtacgaggag gacgtcgtcg cgccggacac
    12421 cgagttcaag ccggacttcg ccggccgctg gatcaggcac gcggagacgc cggcgcgtgt
    12481 gagcgacggc gtcacggcgc tgacgatcaa gtacgcgtac ggcgccggcg ccggcatccc
    12541 cggggtcgag aacgtgccgg ggccgatggg cggcggggcg ggctacatct acgccgccct
    12601 cagcgccgtc caggaggccc gcggcctgag cgaggacgat ctgctgcgcg ggctgttcgt
    12661 ggctgccggc atcggcgcca tcgcctacac ccgcacagag cccaccggcg aagtgctagg
    12721 ctgtacgggc gagtgcggtg tctgcggcgc gatggccgcc gcggcgatcg cggagatggt
    12781 cggcgggtcc cccgaggctg ttgaggcggc ggcgtcgctc gccctgcagg cgttcatggg
    12841 cctgccgtgc gacccgatgc ccggtggcct cagccagcct tgccgtagcc gaatcatggc
    12901 ggccacgtgc acggcacacg tgttcgccga cctggcgctg gccggccacg aggccgtgct
    12961 gccgctgcac gaggcgatcg acgtggccga ccgcatcggc cgcgcgctgc cgcccgagtt
    13021 gctgtgcacc tcggagggcg ggtgctgcct ggcgccggcg gcacaggccc gcagggccgc
    13081 gtaccggcgc tggttcgacg agagccgccc cgaggacagg cctcccggca atctgatctg
    13141 accggagccg acgaccgaga ggagaacgac agcttgaccg aggatctgca gtacgtcggg
    13201 ggcaactacc ccgtccacga tgtggcccag aaggtgaccg gggagctcgt ctacggcagc
    13261 gacttcagcg tgccgggcat gctccacgcc aagctgatcc tcagccggat accgcacggc
    13321 atggtcaaac gcatcgacgc ctctcgcgcg gaggcgctgc cgggcgtcgt caaggtcttc
    13381 tcgcacgaga actcgccgat ggaccgcttc tgccgcgcgc ggatccaccc cggtcaggat
    13441 ctggcgcagc acgacgagac gctgttcagt gagcacgtgc gcttcgtcgg cgaccgcgtc
    13501 gcggcggtcg tggcgacgag ccgggcggcc gccgaggcgg ccgcggcgct cgtcgacatc
    13561 gagtacgagg agctgccggc gctgctcacc gtcgacgagg cgctggccag cacggacgtc
    13621 ctcgtccatt ccaccggtaa cgtggcctac gagcacgaga tcgacagggg cgaacgcccg
    13681 gacctcgagg gcgcggtgat cgtctcgacg tcggccacca cgccgcgcgt ccaccacgcg
    13741 gccatcgagc cccacgtctg catcgcgcag ggccacagca ccggcggcat gaccgtctgg
    13801 accacgagcc agggcgtcta cgccgcgcgc accgtgatcg cggagctcct tcgcatggag
    13861 taccaccgcg tgcgcgtcat caaggtcccg atgggcggct cgttcggcgg aaagaccgag
    13921 tacatcatcg agccggtggc cgcgtacctg gcgcgggaga cgcgccgtcc ggtcaagctc
    13981 ctcctcgacc gcgaggaatg catggccgcg accatggtcc ggccggccac gcagaccacc
    14041 atccgcacgg cctgcacccc ggacggcacg ctgctcgact tcgaggcggc gtccacgctg
    14101 gactccggag cgtacgcctc gagcacgccc gactactcca tccacatgtg caagaaggtc
    14161 accaaggtct accgcgtgcc gcactaccgg caccagggtc gcgccgtgct caccaacacg
    14221 ccggtgggcg gcgcttgccg cggatggggc gccccggaga tggcggcggc gacggagatc
    14281 cacatggacc gggtggcggc cgtgctgcgg ctcgacccgg tcgacctgcg cctgaagaac
    14341 atggtcgagc ccttcgacac cgacctcgtg accggcctgc cgctcggcga cgcgcgcgta
    14401 cgcgagtgcc tcgagcgcgg cgccgtcgcc tttgactgga cggcacgccg cgcccgcccc
    14461 gccgggcgtg gccgcctgcg gcgcggcgtg ggcgtggccg ccggggcgca caagaacggc
    14521 atgttcggcg cctttcccga ggccagcaac atgtcgctca agatgaacga ggacggcacc
    14581 ttcgagctca acgccagcct gcacgaggtc ggctgcggca tcgtgacgac gatgagggtg
    14641 atcgtcgccg aggtgctcgg cgtccacccc gacctgatcg ccgcgggcga agccgacacg
    14701 gcgaccactc cgttcgacta cggcacctac ggcagtcgcg tcacctacgt ggtcggagcg
    14761 tgcgcccgcg aggtcgcgga gaaggtcaag cagcgcctcc tcgccgccgc cgccgagctg
    14821 ctcaaggagc ccgtcgtgca cctgttcacg cgcgagggcg cagtgcacgt acgccatgac
    14881 gcggaccgcg cgatcgccta ccgcgacatc gcgcgcctca gccacatgcg ccacgtcccg
    14941 tccatcgccg cgtcgacgtc gtacttcgcg gtctccaacc ccgcctcgta ttcggtccag
    15001 ttcgcggagg tcgaggtgga ctgcgccacg ggcctcgtcg ccgtcaccga cttcctggcc
    15061 gtcgccgacg tcggccgcgc catcaaccgc ggcatggtcg aggcgcagta ccagggcgcg
    15121 gtgcagatgg gcatcggcta cgcgctgtac gagcacctcg acgtcgacga gaaggggcgg
    15181 agcggcgtcg acggcttcaa gaactaccac atcgtcaacg cgcccgacat gcccgacgtc
    15241 caggtgctgc tggtagagca cgagggtgat gacggcccct tcggcgccaa gagcgtcggc
    15301 gagatcgcga cggtgcccac ggcgcccgcc atcgtgaacg ccgtcaacaa cgccctcggc
    15361 accgagctct ccgacctgcc gctcatcccg gaacgcatca tcgcggcgct ccgcggggag
    15421 gaggtggcgg catgcagctc ctgctgaccg tcaacggcat ggtccacgaa gtcgaggccc
    15481 ggcccacggc gcggctgctc gacgtcctcc gcgaccagct cggtctcacc ggcaccaagg
    15541 agggctgcgc cgagggagag tgcggagcgt gcacggtcat cgtcgacggc aaggccgtca
    15601 actcgtgcgt gatgctcgcc gtgcaggccc gcggcaagaa catcctcacg gtcgagggcc
    15661 tcgcccccga cggcgagctc gacctcctgc agcagaagtt cgtcgagtac ggcgccgtgc
    15721 agtgcggcta ctgcacgccg ggtatgctga tgtcggccaa ggcgctgctc atgggcaacc
    15781 cggtcccgtc cgaacaggac atccgcatcg cgctggccgg gaacctctgt cgctgcaccg
    15841 gctactccgc gatcgttgca gccgtcaagg cggcgagcgg ccaggaggcg gtgcctctga
    15901 tggagctgcc cggcgacgcc gaggagctcg ccgctgaggt gaccaccgac cccgagacgc
    15961 tcgctgcggc ctgcgaggtc gcgcaggcgg acgccggcgg gccgctcggg gccccgtgcg
    16021 actcggacgg tccccctccg gcgtccgagc cggaaggggg cgagtgacat ggcgagcacg
    16081 caggtgctca cccccacaag cgtcgaggag ctggtgacgg cgctggggca ggccacaccg
    16141 cggacgcggc tgctcgccgg cggcaccgac ctggtgcgcg ccatccgccg ccccggaggc
    16201 gagccggatc tgctcgtcga tctcaccggg gtcaccgagc tcgcttccgt ccggctccag
    16261 ggcggctcgc tccgcgtcgg cgccctggct acgttcacga agttgcagtg ggacccgctg
    16321 gtccgcgagc acgcgctgtg tctcagcctc gcggcggcgc aggtcggctc ggcgcagatc
    16381 cgcaacgccg gcaccatcgg cggcaacatc gccaacgcgt cgccgtgcgc cgacggggcc
    16441 acggcgctca cggcgctggg cgccgaggtg accacggtcg acggcgccgg cgcgacgcag
    16501 actcgcccca tcggcgacgt gctgctcggc ccgaaccgca cgggtctgcg acacgacgag
    16561 gcgatcatcg agttcacgtt ccctgctctc ggggccgcgc atcgctcggc gttcgccaag
    16621 atcggctcgc ggacggcggt gagcgtcgcc aggctgagtg cggcgctcgt ggtgcgcttc
    16681 gacgcgccct cgggagccat cacggacgtg cgtgcggcgc tgggcgcggt cggcgcagtg
    16741 gcgttccgcg cccccgagct cgagcgggcg ctggagggac gtctggcgga cgagaagtca
    16801 gcgcgcatct tcgcggaagg ctgcgtggcg gccgtgcgga agtccattcc cggccgctac
    16861 tccctgccct acaagcagca cgccgccgtg gggatcgcct atgacgcctg gaacatgctg
    16921 gcgctcagtg cgcccttcga gcctgcctgg gactgatgca cggaagcgct attgagcggc
    16981 cgtcgacccg aagggccaca cggccggact aggcggaatc cgccaagcga gaagatcggc
    17041 ccaggcccgg ccaagaacgt cggctgggcc cgggccgatc cccggatcgc gagtcagggc
    17101 ttctcgccca ctggcccagc cggcagtgcc cgtcaggcgg tgacgtaggc gttcgtcaga
    17161 tcgaggtacg cttcgaggcc ctgcacgttg tgcaccctct tcgtcgtggc tcgcaggcta
    17221 tccatccaga aggcgaggat gaccggcgtc tcgtcctgct ggatcgtgcc catcttcgtg
    17281 gcgatctcgc tcctgctggc ctcgtctaca gtcgccgagt gctgcttgaa cagcgtatcg
    17341 aactcggaat tggcccagtg ggacgagttc cacacggacg ccttcgtcag catggcctgg
    17401 atgtactgcg ctgccacggg gcgcggcgac cactccacga tggtcatgtc ggcgttgagc
    17461 cagggggtgg tgctggagtc cccaccgtag aactcggtgg cggacacggt gttcagcttc
    17521 acctcgatgc ccgcaggctt gcactgctcc gcgatgatct gggcgtactg cgcgaggtcg
    17581 aggtaggagg aggcggtggt gagagtgagc ttgacgccgc cggcgtagcc ggcatcgctc
    17641 aggagctgct tggccttgtc gtagtcctgc gcacgatccg cgaacgatgg cgacgtcggg
    17701 tacagaggcg agaagatctg gtcgttgcct tgggtgccct tgccatcgta gaggctcgcc
    17761 atgatcgcct gccggtcgag gcagtacgcc acggcctggc ggacgttctt gtcgctgtac
    17821 ggctccttgt ccacccgcat cgccacttca cggatggcgg tcgagtgcgt cgtgatcacg
    17881 ttcaggtcgg ggttggcgaa cagcgcctgc gacccctgga aggcggtttg ggcctgaaga
    17941 tcgacggcgc ccgactggag ttgcagcgct tgggcctgtg cgtcatcgat gtagacgaaa
    18001 ccgacctcat cgaggtaggg cagtcccttc tgccagtaat tcggattgcg cttgagcgtc
    18061 gcgctctgct tgtcctggta atcggtcagc atgaatgggc cggtgccgac gggcttcttg
    18121 gtgaagtcgc cggcgaagga ccggtggagg atgacggagt tgtagttgcc cgacgagacc
    18181 aggtagggga agtcggcgaa tgcagcgtcg aggttgaact gcaccgtgta gtcgtcgatc
    18241 ttctgcgtgc cgccctgtga gaggatcccc ttgagagccg acagggcgcc ggatccgctg
    18301 tcggggtcca caaggcgatc gaacgtggcc accacatcat cggcggccag gggcgacccg
    18361 tcgttgaagc tgacgccctg gcgcagcttg aacgtccaga ccgcgccggt gggatccggt
    18421 gaccagctct cggcaaggac cggacgcagg gtgaggtcgt tgttgatcca gatcaggtac
    18481 tcgcagacct gctccaccgt cgagatgtcg ccggtctggt acatggtgac cggatcgagc
    18541 gcgctgaggg gagtgagggc ggtcaccttg atggacccgc ccttctgtgg cgccgccgaa
    18601 gcggaaggcg acccttcgga cgtcgtcgtg gagtcgccgc cgcatgccgc gagaaggctc
    18661 ccggcggcgg tcagcgagaa gccgaagact gacgcccgga cgagcagctg gcgacgcgtc
    18721 atccgccccg tccggacctc ctggatcagg tggtctgcgt actccttggc atgccggtcg
    18781 tcttgcatgg agcaacctcc cccgtacggt cagcgcctca aggccgccga aggcgagcct
    18841 cccctgtgga cgctatttgg gcgcactata ggcccaaatc cagcggtgcg caagaatcgg
    18901 caaggctgag cgtgctggcg ccggttcacc cagcggcgcg tcgcagcagg cgttcgatct
    18961 ccccgagggc ctgatcgtcg agcgcgaagc gtggcggctc cgcccgcagc tcggcgacgc
    19021 gctgctggac gcgctgtttc agcgaagtcg cgccgttgtt ctgccagcga tcgtgcgggg
    19081 ccttgtcgag cagcgtgggc gtccagaatt cgcgatagtg cgtgcgggtg taccgacgag
    19141 gcagatggtt gccgccggga ccgactgaga tgatctcgtc gacggcgaga gcctcgtcgt
    19201 cgacggcgat gtcgcgcagg cacgccttgg cgaagccggc gagctcgtcg ccgagcacga
    19261 tggactcgaa ggacgtctgc atgcccatct cgagatagcc gacgtcgtgc aggagcgtac
    19321 ctcgggagag ggcgcccatg acggcggtca tcgcggtctc ggccgaccac tgttcgtcca
    19381 gcatcttggc atccgaatac ccgccgtagg agaagctcgg tagcgagtag aagcgggcca
    19441 ggtcgcaccc cgcctggcag cccatggacc attccggcga gacgtaggga ttgaccatcg
    19501 tgcgcatgtc cagcgtgtcg cacccggctc cgtagacgaa cggagctccg gggttgacga
    19561 gctgatgaag gacgagaccg ctcaggacct ccgcgttccc ggtgaccacc acggcggcga
    19621 tggaggcagg ggcggtggag ccggctgtcg gcgccggcgc gtagacgagc gggatcatca
    19681 gttcggcgca cgtctcgatc ttggagaggg cgaactcgtc gtgcatcagg ggcggcgacg
    19741 gcatgccgta gacgatgagg ctgtcgccac cgccgcaggc ctcggccatc gccttcatcg
    19801 tcgccagggt gtggccgtcc cggggcacga tgagaagcgg tttgcgcgtg cctttgagca
    19861 gggcgacctg ctggtgaagc acctcggtct cgggcggcac gttctcgggg aatcccatcg
    19921 acatgacgaa gtcgatgttg tcgagcttct cgcacaagcc cgccagggcc tcgatgtcgt
    19981 ccacgacggc tctgcgccgt tggcctgtgt ccgggtcggt cacgtacaga cagtcggatc
    20041 ccgagccgaa gtaggtgggg ccgtccttga gctcgagcac ttcgtcgcgg cccctcgatc
    20101 ttacggacca cgtgcgaggg gccgacgcaa gggcccggct cacgagctcc gagctcagcc
    20161 tgacccggct gccgtcgact ttagcgcccg cctctgcgaa gcgtcggagc gccggttccc
    20221 acatgacctc gaccccgcac tccgagagga cacgcaaggt ggcctcgtgg acgagctcgc
    20281 actcctccgt gtcccacacc cggatggccg gcttacccac cgtctacctc cacctgtcgt
    20341 catcgtcgca ggaaaggtgc gcccgcgagg gggttcagag gactgctctc gggtcacgtc
    20401 gcgcaccctg tgctaaatgg acctagaccg agtcctatca ggagtctcgc ataggggaca
    20461 aagaccgtca agactttgcg cacggcggtt gtccgggagg cacaggtgtt tactccgggc
    20521 cagaagactg cgaagctggc gcggatggct gcaccgaaag ctcgaggacc aggagtgcgg
    20581 atgaggggtg aagatgagag cgtccccggc gtgaagccgg tactcgatct tgggccgctg
    20641 cggcctcgct cctcgtcgat gctcgtggcg gactcgctgc gcaggctcat cgcactgggg
    20701 gccctctcgc ccggggagcg tcttccctca gagcgggagc tggccgagac gttgcgtgtt
    20761 ggccgcgact cgatccgtca ggcgatacgg accctgaacg atgaagggct cgtggagacg
    20821 cgactcgggc gcagcggcgg gaccgtcgtc ctggacaggc cggtccagcc gaagcgcctg
    20881 tcggaggaga tcctggcaag ccatcgcgaa atcagggcaa gttatgagtt ccgccttgcg
    20941 atcgagcccg ccgcggcatc catggccgcc cggcgggcga ccaccgagga tgtcgcgagg
    21001 atcaccgcca tcgcggacga gcaggcttgg agcttccgct cctggcgcag catcgattcg
    21061 cggtttcacg ccgcggtggc cgaagcgtcg ggcaacgggc tcctgctcga cgccgtccag
    21121 cgcacccgga cggccttctt ctcctggtac gacgcgatct actctcgctt gccgtgggac
    21181 tccctgccga tccaggaccg ggatttcgcg tacttgcatc ggcccatagc cgaggcgatc
    21241 gcagacggcg atcccgagcg ggccggcctg ctcatgaaag aagcgctcga gtggtcggag
    21301 caggatctcg aggatctgct ggacggcgtc gtcgctcacg cgagcgcacg ccgggagaac
    21361 gtgccgcgga gcatggagcc cgaccccgcg ggacagacgg caccgggagc tgcagacgct
    21421 cgacgcgctg cagaccggga cggctgaggc cacgcctgtg gcgcgaggac ggtcgcgacg
    21481 ctgccgcaat caccccgccc cgaagtgcct gcccccgatc tcgacccact ggccgaggcc
    21541 ccgggccttg gccgcctcgt acacgtagtg ggcggccgcg aggtcctcga tggcgatacc
    21601 gagcgacttg aacagggtga tctcctgcgg tgacgtgcgc acgggggcct tgccggtgag
    21661 gacctcgccg atctcgccga cgatgtgctc gtcgccgaag agcccctcgc ccttggggat
    21721 gaggaattca ccggcttcgt tgagcagcga ctcgcgccgg tcggcgtaaa ggcgcgactt
    21781 cgccacgagt gccgaatcca gctcgcgcgt cgccgggttg taggcgccga cggcgttgat
    21841 gtgagcgccc gggctgaccc agtcgccgtg gacgacgggt tcgtgggagg tcgtgaccgt
    21901 gcagatgagg tcagcgccct cgactgcctg ctcggcactg gccgcgacct cgatctcgat
    21961 tcccgtgcgc cgggactcgc ggtccgcgaa catcgccgcg ctctccgccg gcacggagta
    22021 cacgcgcacc ctgtggacgg gacgcaccgc cgccatggcc tgcagatgcg tgtgcgcctg
    22081 cgtgcctgcg ccgatgatcg ccagatcccc ggcgtcggga ttcgcgagca gatcggtgac
    22141 cagaccgctc acggccgccg tgcggatggc ggtgacggcc gtcgcgtcca ccatggcacg
    22201 cagcaatccc ctctgggtat cgaaaaagag cacgacgccc tggtgggtgt cgtactcgct
    22261 gccgaagttg gccgggaact cggcgatgac cttgacgccg acggcttcca agccgccgag
    22321 gtacgacggc atcaagccca tgacggcgcc gcccttcggc aggtggagca tcgaacgcag
    22381 aggcatcatc acctcgccct ctgcgagcag ggtcaaggcg cgcctcatca cgccgatggc
    22441 gtcttccatg ggcagaagtt cggtgacgag gtcctggtca gcgatgagca cactcatagc
    22501 cacttctcct tcgcatgtct ttcagcggcc cgggcgatag cgtcgcggtc cagctccacg
    22561 cccaagccgg gagtctcaag cgggcctatc ttcccgcaga tcggagtcag atcgggggag
    22621 accaccaggt cctcggcgat gagctggtgc atgatctggt ttccttcgct gaggtttgag
    22681 gtgctcaggc cgaggtggtg ctgggccgca tcgctgatgc tcgagacgtt ctgaccgtgc
    22741 aggcagaccg gcaagcctgc ggcctccgcg atagccgcag ctttggcgaa ggcgtgcaga
    22801 ccgcccgcct cgtgcgccga gagcacgata gcgtcggcgg cgcgctggcg gcagatctcg
    22861 tagacgtcgg caggagtgaa cgctgcctgg tcggcggcga tggggacggt cacgccatcc
    22921 ttcacctgac gcatcgcggc gacgctggtg ggcggcgtcg gctgctcgat ccattcgggc
    22981 tcgaatggcg cgaggcgccc gatcatgtgg atcgcctccg tcacgcccca tgccccgttg
    23041 gcgtccagac ggaggcggcg agcgccgatg gcctcgcgca ccgcggccgt gttgcgcagg
    23101 tcggcctccg cgccgcggcc gaccttgagg tagatcacgg agtatcccgc cgccgccaag
    23161 tcacgggcgt cttccaccaa ttcctctgtg gtgtcgccct ggacgaaacc gaagtagtcg
    23221 acgtcgtcgc gtaccgagcc gcccagtagc cggaacaccg gccagccggc agccttgccg
    23281 agtacgtccc agagcgccat ctcgaatccg gcaagggcaa ggttggccgc tcgaggcgtg
    23341 tggttgaagc tccccaggta gcgcgccttg gtcaccagcc gctcgacgtc aaaagcgggc
    23401 tcaccgacca gcagatgggc cacgctcttc agcgcggcta ccgtgctgtg cgcggggatg
    23461 ccggctgtcg actccccgac tccgaccacg ccctcgtcgg tgaagacttc gacgagtacg
    23521 accgcagcgc cgtagtcgat ccgtccggcc cagtggtacg gctccttgaa cggtagccac
    23581 agaggagtcg tggagatgtc ggtgatcttc aaggtcgtcc tcctcgatcc cgggtgtgag
    23641 ccgggcgttg ctggcctcgc gccggtacga accggaatcc atcgctgcgc cggccgcccg
    23701 gtcaatcgcc gtcgcttggg tgcgtaaatg gacctccaag aaggccgttt aagagatatc
    23761 acgggcaccg aatcgcggtc aatggcctgc ggcgcggtgg aagaaggtga accccgatgc
    23821 tcgctggcgc gcgcggccgc agcagcagct cgcgcctcat tcaggccgtc tcgcgcggcg
    23881 ctccgcgcgc gtcagtcggc cggcgccggc tcgggctgag gcgccggccg gtcgtgcatc
    23941 tgcagcgcgc gtaactcgtc cggcgggatg tggcacttgt acgtgtgccc gttgcccgcg
    24001 tccatcgccg ctggctcctg ctccacgcag atctcgccga ggaaccggtg acaacgcgtg
    24061 tggaagcggc agcctgacgg cgggtcggtg aggctcggca tgctgccgcg cagcgggatg
    24121 cggtcctgct gcttctcgaa gtcgaggcgc gggatggagc tgaggagggc ctcggtgtac
    24181 gggtggtgcg gtgcgctgaa cacatccgcc gccgccccca cttcgatgag gtcggcgagg
    24241 tacatcacgc cgatgcggtc cgagatgtag cgcaccacgg cgagatcgtg gctgatgaac
    24301 acgtaggacg tccgctcctt ggtctgcagc tcgacgagca ggttgaggat gctcgcctgc
    24361 acggagacgt cgagcgccga agccggctcg tcgcagatga ccaggtgggg gtcgccggcg
    24421 aacgcacggg cgatcgcgac gcgctgcttc tggccgccgg agagctcggt gggccgctgg
    24481 aacaggaacc gcggctcgac gcgcacgtct gcggagagct tgtcgacctt ggcgcgcacg
    24541 gagccttcct tggcgccacc cagcttgcgt acggcgcgcg tgaggatctg ccgcgtgctc
    24601 caggccgggt tcagcgtgga gtccgggttc tggaagacca tctggatggc gcgcagggcg
    24661 tcctgggagc ggcgtccggc cttggccttg agcacgccgc cctcgaacgt gatcgtgccg
    24721 gagtcgggag gaagcagacc cgtgacgcac ttggccagcg tcgtcttgcc gctgcccgac
    24781 tcgccgacca ggccgaaggt ctcgcctttc ttgaggctga gagagacgtc ggcaacggcg
    24841 gtgagttggt tgcgcccgtc cttgaacgtg cgcacgaccc tgtcgatggc gaggacgtcg
    24901 ctctcgccgc cgcgctcctc caggctggtg gccaggacgg gggtcgtgtg cggcatgccc
    24961 ggcgtgtccg cgcagaagta gcagcgcgcg tgccggtcgg agtcgcccag cggctcggag
    25021 tggtgtggcg agtaggccgc cgggtcggga ggcatggcct cggcctccga gcgacccctc
    25081 tcgggtgcat cactgggcca ggcgaagaag tcgggttcgc ggcgcgtgca gacgggcttg
    25141 gccatgggac agcgcgccga gtacacgcag ccctcgagag gatcgccgag cgccggcagg
    25201 gtcccgggga tggtctgcag ggcggcgtcc gtcttgttca tcccgaaccg cggcacgcag
    25261 cgcagcaggc ccatggtgta cgggtgccgc gggtcggtga agacctcctt ggccggcccc
    25321 tcttcgacga gccggccggc gtacaggacg ccgacacgct cgcagagacg cacgaccagt
    25381 ccgaggttgt gcgtgatcag caggatggcg gcgttgatgc gcccgcgcag ctcctcgatg
    25441 aggtcgagga tctccgcctc cacggtggcg tcgaggccgg tggtcggctc gtcgaggatg
    25501 agcagctgcg ggttcaccgc gagcgccatg gcgatgacca cacgctgctg ctgtccgccg
    25561 gagagttcga agggatagcg cttgagcgtc ccgtccgggt cggggatggc gacgcggcgg
    25621 aggctctctc gcgccttctc gagcgccgcc ttcttgtcca ggccctcgtg atagtggaag
    25681 acctcggcga gctgatcgcc gatgcgcatc gcgggactca gcgcctgggt cgggtcctgg
    25741 tagaccatgg agaccttgcc gccgcgccac ttgcgcagcg cctcttcgct gagctcgctc
    25801 acgtcctgcc cgtcgaccag cacccgtccg ttgtcgagcg taccgttggc ggcgaggtag
    25861 cgcatcgcgg ccatggccag cgtggtcttg ccgcagcccg actcgccgac cagcccgtag
    25921 gcctcgccac gctcgatctt gagcgtgacg cccttgagca ccggcagcct gcggccgcgg
    25981 cgatggaacg tgacctcgag gtcgctcacg tcgagggcgg cggcgtcggt catggccggc
    26041 tgctgattct ccacgctgct catttggaga acgtcctccc cagtccgtcg gtgatgaggt
    26101 tcacggccac gacgagagaa gccagtgcgg ccgcggggaa catcacggtc cacggggcga
    26161 tctgcaggaa gatgcgttcg ttggcgatgg tgagccccca gtccggcgac ggaggcacca
    26221 agccgaatcc caggaacgac agcgtggcgg cggtgaacac agcgtagccg agccggatgg
    26281 tgccctcgac gatgatcggc tgcgtgatgt tgggcaggat ctcgcggctc atgacgaaga
    26341 ccgagcgctc gccgcgcaga cgcgccgcca tgacgtactc gcgctcgcgc tcgccgatga
    26401 ccgccgccct gacggtgcgg gagaccacgg gcgtgaagag cgcgccgatg gcgatgatga
    26461 tgatcgccgt ccccttgccg aacagcgaga ggatgaggat cgaggtgatg atgatcggca
    26521 gcgcgagcag cacgtcgatg gcgcgcatga tgatctcgtc cgtgatgccc cggtagaagc
    26581 cggagatgag gccgatgatg ccgccccaga gcagggcgag cagcgtggcc agcggcgcga
    26641 tgatgagcac cgaccgcgcg ccggccatcg tgcgcgcgaa cacgtcgcgt ccgaggtcgt
    26701 cggtgccgaa ccagtgggcc gcgctcggcg cctgaaggga gctcgcggcg tcctgtgcgg
    26761 tgaagctctg cgtgatggac gtggagaaca gcgccatgaa cacccagaac agcacgatga
    26821 cgaggccgat catgaacgtc gggctgcgca ggagcgcctt gaccagatag cggcgctgcc
    26881 gcttctgctc cgccgcggcc gcggcgtcgg cctggagctc gccgtccttg gacggtccgt
    26941 cctc
//
LOCUS       DASUMX010000283         4233 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_105864, whole genome shotgun sequence.
ACCESSION   DASUMX010000283 DASUMX010000000
VERSION     DASUMX010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4233
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105864"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /locus_tag="VFZ86_13465"
     CDS             complement(<1..645)
                     /locus_tag="VFZ86_13465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643325.1"
                     /translation="MSIAERDYRVLTVGLALCAGLAVAYAALFPGPVPVSSAVIRDAL
                     VFAVLAIIADEMSVEISERVTLAAFNLPILLAIMFTGRLPAIGVAIAVGLWGAWRENS
                     RSVVIYNTANFVVAVFLASLAFDALLGPLEVSLSDVTVGLLAAGAVAAVCFEATNLAL
                     LSLGMRVKYRRAFRAFWRQEMPPFLRSLGVLLLLGLAIAALYAAAGIIAVALLFV"
     gene            1207..2433
                     /locus_tag="VFZ86_13470"
     CDS             1207..2433
                     /locus_tag="VFZ86_13470"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5643326.1"
                     /translation="MEANAVAAETQFRPLYRNASFLLLWAGQFVSQIGDRLAALAFPW
                     LVYQRTGSALGTGAVFAVYTLPYVLFGAFAGVAADRFDKRRLMIAADLVRAALVLAVP
                     FVAARSLPAVFVLSFAIASAGVFFDPAKLAMLPEIVQPGRLLRANSLLSTGENLTEIL
                     GWAFAGLLLASVSTSVAFGLDAVTFAVSAAALALMRYKPTMGAAAERTARAFWGELKD
                     GFRFLGRDRGLRANTVMIIVCVAGLGATYPLTFLFAVQVLEGGAGAFGALEAAVGVGY
                     LAGSLALVALATRVHKGRVMIAGLALMGACLALVPTTDSVWTAAVPFAVFGLANAAVL
                     IAVDTYLQEVVPHGMRGRVLGTRFTITQGMYALSVLIGGALAAVVDVRVLFIVAGAIV
                     ATSALVGLLTREVRDS"
     gene            2545..3873
                     /locus_tag="VFZ86_13475"
     CDS             2545..3873
                     /locus_tag="VFZ86_13475"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5643327.1"
                     /translation="MTSPNDTQKTGDAGAPRTSWMQLGVVSAAEFVVWTGFGAIMPYL
                     PIFLKDEAHSSLFMIGLIAAAFYLGTLLFSSPFGWLSDTIGRKPVMLFGVVLYALAMF
                     LFTRTSDPWWFVLFRLLEGIGTAAVAPAAMAFVADITTERNRSKAYGILTSAQFGGLI
                     AGPALAPPLYHYFGGGRDGFYAIFYFGAILTAFTAVAVLAFIREPTHVTARREARRGR
                     QAERPPYRAILSPAIIAFLVVGFTSHLSMGAFEVVWSIWLDHLGASMTYISLTWIAFS
                     VPMLLAFAGGMLADRYSRFALMFAGYAISGVAWILYGTTTNLVLFLAVNVVEGLAIAF
                     SYPAKQAFLVQVSPSKWLGTVTGLETTSMQLAGMIGTFLAPVVYGWISGYVMAVGGVL
                     SLAGLAAAAPVLRREWRRVSAAGPAPPGDELAGRDGAQPTAAAQPPSGIE"
     gene            complement(3920..>4233)
                     /locus_tag="VFZ86_13480"
     CDS             complement(3920..>4233)
                     /locus_tag="VFZ86_13480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dehypoxanthine futalosine cyclase"
                     /protein_id="HEX5643328.1"
                     /translation="TALSEGHVPATGSDYRRLLAVSRLYLDNVPNVQASWVTQGLRMG
                     QVALAFGANDMGSTMIEENVVAAAGVRNQCDVDEMVRLIRAAGRTPVQRDTLYREVRR
                     Y"
BASE COUNT          641 a         1465 c         1404 g          723 t
ORIGIN      
        1 cacgaagagc agggcgaccg cgatgatgcc ggcggccgcg tacagcgcag cgatcgccag
       61 tccaagcaga agcagcacgc cgagggagcg aaggaacggc ggcatctcct gccgccagaa
      121 tgcgcggaac gcgcgacggt atttcacacg cataccgaga ctgaggagcg ccaagttggt
      181 ggcttcaaag cacaccgcag ccacggctcc tgcggccagc agcccaaccg tgacgtcact
      241 gaggctgact tcaagagggc cgagcagcgc gtcaaaggca agagacgcca agaacacggc
      301 gacgacgaag ttggctgtgt tgtagatgac caccgagcgc gagttctctc gccaggcacc
      361 ccagaggcca acggctatgg cgactccaat agccggaagt ctgccagtga acataattgc
      421 gagcagaatt ggaaggttga atgccgccag ggttacgcgt tcggagatct ccacagacat
      481 ctcgtcggca ataatggcca agacggcaaa gacaagcgcg tcacgaataa ccgcagatga
      541 aacgggaacg ggtccaggaa agagagcagc gtacgccaca gcaagcccag cgcagagtgc
      601 caggcccaca gttaggacgc ggtagtcacg ctcggctatc gacacggtac cttctcgggg
      661 tctgcagtgc cccgtttatc ggcaccgatg aaacggagct tgagggctca gtgcatcttg
      721 ctgccgccgc cgctcgtcac gatggcgaac agcagggagg cgagcgtctt ctcgatcttc
      781 ttgctcatga tgttacctca gtcgatcgat cgctcgctgt cagtgcatct tcccgccgca
      841 acggatgtgc atcttgccgc cgaacctgat gtgcatcttg ccgccgagcc gcattgtccc
      901 ctccgagctt cgctaaaggt tccgcaacca catgccgata gcttgtggtt gacgatcagt
      961 gcgctgactc tatggaggcc agtctggcct cgcatacccc actgactagg acataatggg
     1021 caacgttcga ccccagagtt gccaatgtgg acaacacatt gaggaagggt cgcggcgggc
     1081 gcaggcgcac cgcgacatcg tcaacgtgtt gatgtacgct tcgaggacgc ggccgctcgc
     1141 acacggccgc gttcgcacgc gtcagagcct catcacagga cacgacgtgc ttcaggggga
     1201 agaccgatag aagccaacgc cgtcgccgcg gagactcagt tcaggccgct ctaccgcaac
     1261 gccagcttcc tgctgctgtg ggcgggacag ttcgtctccc agatcggcga ccggctggcc
     1321 gcactcgcct tcccctggct cgtctaccag aggacgggct ccgccttggg caccggcgcc
     1381 gtcttcgccg tctacacgct cccctacgtc ctcttcggcg cgttcgccgg cgtggccgcc
     1441 gaccggttcg acaagcgccg gctcatgatc gccgcggacc tggtgcgagc cgccctggtg
     1501 ctcgcggtgc ccttcgtggc cgcccgctcc ctgcccgccg tgttcgtgct gagcttcgcc
     1561 atcgcctcag ccggcgtctt cttcgacccg gccaagctgg cgatgctgcc ggagatcgtc
     1621 cagccgggcc ggctgctgcg cgccaattcg ctgctgtcga ccggcgagaa cctgaccgag
     1681 atcctcggtt gggcgttcgc cggcctgctg ctcgcgtcgg tcagcacctc ggtcgcgttc
     1741 gggctcgacg ccgtcacctt cgccgtgtcg gcggcggcgc ttgcgctcat gcgctacaag
     1801 ccgacgatgg gcgcggcggc ggagcggacc gcgcgcgcgt tctggggaga gctgaaggat
     1861 ggttttcgct ttctggggcg cgaccgcggc ctccgcgcca acaccgtgat gatcatcgtc
     1921 tgcgtcgcgg gactcggcgc cacctacccc ctcaccttcc tgttcgccgt ccaggtgctg
     1981 gagggcggag ccggagcatt cggagccctc gaggcggcgg taggcgtcgg ctatctcgcc
     2041 ggctccttgg cgctggtggc cctggcgacg cgcgtgcaca agggcagggt catgatcgcc
     2101 gggcttgccc tcatgggcgc ctgtctcgcc ctggtgccca cgaccgactc ggtctggacg
     2161 gcggccgttc cgttcgccgt cttcgggctc gccaacgcgg ccgtgctgat cgccgtcgac
     2221 acgtacctcc aggaggtcgt gccgcacggg atgcggggca gggtgctggg gacgcggttc
     2281 accatcaccc agggcatgta cgccctctcg gtgctcatcg gcggcgcgct ggccgccgtc
     2341 gtcgacgtgc gcgtgctgtt catcgtcgcc ggcgccatcg tcgcgacctc cgcgctcgtc
     2401 ggcctcctca cccgcgaggt ccgggactcg tagcacacgg aggctgcgcc gtcacctgac
     2461 ccggtgatag ccttgcggcg gatcccgcag cccgacggac cggggcaggc ctgccgtcag
     2521 accttgcggg aacgcagccg atccatgacc tcccccaacg acacgcagaa gaccggcgac
     2581 gccggcgcac cgcgtacgag ctggatgcag ctcggcgtgg tcagcgccgc cgagttcgtg
     2641 gtgtggacgg gattcggggc gatcatgccc tacctgccga tcttcctgaa ggacgaggcg
     2701 cacagttccc tgttcatgat cgggctgatc gccgccgcct tctacctggg cacgctgctc
     2761 ttctcttcac cgttcggctg gctcagcgac acgatcgggc gcaagccggt gatgctgttc
     2821 ggcgtcgtgc tctacgcgct ggcgatgttc ctcttcacac ggacgtccga cccctggtgg
     2881 ttcgtcctct tccggctgct cgagggcatc ggtaccgccg cggtcgcccc ggcggccatg
     2941 gctttcgtcg ccgacatcac cacggagcgg aaccgcagca aggcctacgg catcctcacc
     3001 tcggcgcagt tcggcgggct catcgcgggg ccggcgctgg cgccgccgct ctaccactac
     3061 ttcggcggcg ggcgtgacgg cttctacgcc atcttctact tcggcgcgat cctcacggcg
     3121 ttcaccgccg tcgccgtcct cgccttcatc cgcgagccga cgcacgtgac tgcgcggcgg
     3181 gaggccagac gcggccgcca ggccgagcgg ccgccgtacc gcgccatcct ctcgccggcg
     3241 atcatcgcct tcctcgtcgt cggcttcacg agccacctct ccatgggagc cttcgaggtc
     3301 gtctggagca tctggctcga ccatctgggc gcctcgatga cgtacatctc gctgacctgg
     3361 atcgccttca gcgtaccgat gctgctcgcg ttcgcggggg ggatgctcgc cgaccgctac
     3421 agccgcttcg cgctcatgtt cgccggatac gccatctcgg gcgtcgcctg gatcctctac
     3481 gggacgacga cgaacctcgt cctcttcctc gccgtgaacg tggtcgaggg gcttgcgatc
     3541 gcattctcgt acccggccaa gcaggccttc ctcgtccagg tgagtccgtc gaagtggctg
     3601 ggcacggtga cggggttgga gacgacgtcg atgcagctcg ccggcatgat cggcacgttc
     3661 ctggcgcccg tggtctacgg ctggatctcc gggtatgtga tggcggtcgg aggcgtcctc
     3721 agcctcgccg ggctcgccgc cgccgccccc gtgctgcgcc gggagtggcg gcgcgtcagc
     3781 gccgcggggc cggcgccgcc cggcgacgag ctcgccgggc gcgacggagc gcagccgacg
     3841 gcggccgcac agccgccctc cggcatcgag tgagaccgag gtcgacgagg gccgcattcg
     3901 cggcggagcc ggcgcggcgt cagtagcggc gcacctcgcg gtagagcgtg tcgcgctgca
     3961 ccggcgtgcg gccggcagcg cggatgagcc gcaccatctc gtcgacgtcg cactggttgc
     4021 gcacgccggc ggcggcgact acgttctcct cgatcatcgt cgagcccatg tcgttggcgc
     4081 cgaaggcgag cgcgacctgc cccatgcgca ggccctgcgt gacccaggag gcctggacgt
     4141 tgggcacgtt gtcgaggtag aggcggctga cggcgagcag acgccggtag tcgctgccgg
     4201 tggccggtac gtggccctcg gagagggccg tgt
//
LOCUS       DASUMX010000284        19733 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1831335, whole genome shotgun sequence.
ACCESSION   DASUMX010000284 DASUMX010000000
VERSION     DASUMX010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 19733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19733
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1831335"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..553
                     /locus_tag="VFZ86_13485"
     CDS             80..553
                     /locus_tag="VFZ86_13485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX5643329.1"
                     /translation="MADRQLEARVICGKDDEDLLDHCDVHVVDPERVTAVRERLVSPA
                     VGEQMANVFKVLADQSRCRLMAAIIEAGELCVCDLAATVDMSESNVSHHLRVLRANGL
                     VRARRAGKMVFYSPDDAHIRLLLDITREHVEHGPEDLSGEAVVSGRREPATAPGR"
     gene            558..3161
                     /locus_tag="VFZ86_13490"
     CDS             558..3161
                     /locus_tag="VFZ86_13490"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEX5643330.1"
                     /translation="MTARTIELSLLLPRAAECGGCVTEVGRELLRLDGIHAVEPDVPR
                     GLLRLTYDEAAVTGDDVETFARRAGAQAHCADHCPLAVHEHGPLDLSQALAVEDHGER
                     RILHVTGMDCADCAVKLQGALRRERGVSSADVNFGAATLAIAIDPSQTALPDVFRAVR
                     RLGYDTVERRGAEEDPGARRGAAATAGRGFWLSEPRAIATLMSGALTVAGFVATAAAP
                     EAAPWLFGAAMLSGGLYVARAAVFSLRGRQVDMNVLMTLAMIGAAAIGEWSEAALVAF
                     LFSLGTVLQVATLERTRRAISGLMKLAPPTAAVLRPAETGAALREAKVDVADIVVGDV
                     VLIRPGERAAVDGVVVEGAAAADQAPVTGESAPVAKGPGDQVFAGSIVEGGTLRVRAT
                     AAASDTTIAKIVHMVEEAQAQRAPAESIVDRFAGRYTPVVVALAAAVAFVPPLFGASF
                     DTWFYRGLALLIISCPCALVISTPVSILAALARATKNGVLIKGGAYLEQMAGLKAVAF
                     DKTGTLTLGRPQVTDVVPLHGTSPDRLLTVAAALERYSEHPLARAITERAAQDGSAQA
                     VAAAAPPAEVAAAACSCSEPGCCTAGADAAQVADGDAARGHAPHDAADAHLHRGPDVE
                     RFRAIPGRGVRAELDGRLHFAGRPDLLGAHAFDPALTAAVSRLEREGKTAVVVGDEEG
                     PLGVVAVSDPLRPGAHEAVAALRDLGVAEIAMLTGDNPETARAVAAQAGVTDVRAGLL
                     PAQKVDAVVELRDRAGAVAMIGDGVNDAPALAAASLGVAMGAAGTDAALETADIALMG
                     DDLSAVPETIRLARRTTRVIWQNIVFSIAVKAVFLVLAPLGLVSLWLAVFADMGTSLL
                     VTANGLRLYRR"
     gene            3240..3452
                     /locus_tag="VFZ86_13495"
     CDS             3240..3452
                     /locus_tag="VFZ86_13495"
                     /inference="COORDINATES: protein motif:HMM:NF041212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxx-star family glutaredoxin-like
                     (seleno)protein"
                     /protein_id="HEX5643331.1"
                     /translation="MTTIIYTKPGCPYCAAAKQDLDARGITYDEIDVYTTPGARDQLA
                     ELTGGMNVVPVVIEEDGEVRVAAGGG"
     gene            complement(3490..3849)
                     /locus_tag="VFZ86_13500"
     CDS             complement(3490..3849)
                     /locus_tag="VFZ86_13500"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX5643332.1"
                     /translation="MWPFKKRPEGAATAPVAVPTIDVRQAFARSKRGARLIDVRSARE
                     YAHSGHPKGAISVPPQLISKDRTGLGRDDDVLVICLSGHRSPRQARKLAEMGFTRVTN
                     VHGGLMAWKKAGLPVKK"
     gene            complement(3863..4231)
                     /locus_tag="VFZ86_13505"
     CDS             complement(3863..4231)
                     /locus_tag="VFZ86_13505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643333.1"
                     /translation="MLIGQTMFSVRHGTEHRAERALQELAGLLQGTAGHTSHRVLRSF
                     GMSPLGSALRDEGREATLGDVHFVFETEWDSLQAHDDFYQGAQVQLVYGTLQSILTSG
                     PFEVLYDAVVEEPQHDGAAV"
     gene            complement(4301..4753)
                     /locus_tag="VFZ86_13510"
     CDS             complement(4301..4753)
                     /locus_tag="VFZ86_13510"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX5643334.1"
                     /translation="MGYDFGVGREAPTFTLSAVDGSEIKLNQYRGDWFPVLVFIPTRS
                     PGASGLLEQLSETADAVWGLRGQIVGICDAGRDECKTLAAGVPGLAFPLLPDDGSVAR
                     QYGALRADGTARPMAFIIDRAGKIVWTGDGAAALTTAALLAAFRQVVR"
     gene            complement(4851..5219)
                     /locus_tag="VFZ86_13515"
     CDS             complement(4851..5219)
                     /locus_tag="VFZ86_13515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643335.1"
                     /translation="MRGAAVVFAVAAPVTLLVVTAAAADGPRVGPPPPGARVAPLAAV
                     QASATRKEVAGAVATSHRGTRLRAHDAATPPSPETAPAMSAGLPLAAAPGGPREAAIT
                     LPGARAPPAAGRAVAQFWTA"
     gene            5460..6188
                     /locus_tag="VFZ86_13520"
     CDS             5460..6188
                     /locus_tag="VFZ86_13520"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEX5643336.1"
                     /translation="MRRVTTVLIAALALLGVAAASLTVAACGGESVPSNAVATVGDVT
                     VTKADFQDLLTQAQVQMKAQGMNVPKEGSAAYDHYVAQVVNYLVQEQVVEQSAEELGV
                     SITDKEVGEQVAELVQAYGGEQKVVDLLAEQGMTMELLKRSIRSQTLSDRAAEIVTEE
                     ASVSDAEIQMYWEAHKAELSKKKKTATLAKARTTIEQILVSAKKLQLWNAWVAERAEA
                     LGVEYASGYDPTLLTPSASASPAD"
     gene            complement(6312..6857)
                     /locus_tag="VFZ86_13525"
     CDS             complement(6312..6857)
                     /locus_tag="VFZ86_13525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HEX5643337.1"
                     /translation="MDEAMEKRLPKAEELSAMTKESLAAAFAGESQAAEKYLVWADQA
                     DEEGYPNVAKLFRAISFAETRHARNHLRVMGGIGSTADNLAAAFGGESFEVDEMYPAY
                     HGIAKLQGNNQAVRSINYALKSEVDHKGQYTDAREQVLAGKDISDQPVSVCLVCGHTV
                     IGDVPEKCPYCAAGKELYKSF"
     gene            complement(6857..7405)
                     /locus_tag="VFZ86_13530"
     CDS             complement(6857..7405)
                     /locus_tag="VFZ86_13530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015312314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HEX5643338.1"
                     /translation="MAEPFKNTPPAPEAKGDMTAANIAAALAGESQASQKYLAFADVA
                     DAEGYPNVARLFRAVAYAESTHARNHLSVLGGIGTTAENLKAAWGGETFEIDEMYPAY
                     DAVAKLQENESAQVSIRYALKAEMDHQGIYDTTLKGVEGEKDLDTTPVFVCPVCGHTV
                     VGDKAPAYCTQCGTTSEFFKEF"
     gene            complement(7664..8092)
                     /locus_tag="VFZ86_13535"
     CDS             complement(7664..8092)
                     /locus_tag="VFZ86_13535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643339.1"
                     /translation="MARPIPAAFGEKYRRVQKAVTVARGADRRHFDFTGEVELGVRLV
                     LSEAGGTPLAFSLWSLPQDIAELCSDASVPATAALLAIDSEQAREARAAGLTVDLSQF
                     TRSQSRPDVYYTLFDRASPSRVHTALHRLVPALEPHAAAA"
     gene            complement(8250..10169)
                     /locus_tag="VFZ86_13540"
     CDS             complement(8250..10169)
                     /locus_tag="VFZ86_13540"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEX5643340.1"
                     /translation="MNGFLGRGERRRLAARVRRVLPALGVLLLVAAGAAGLAAGPAAA
                     KDFSITSVSVDATVKRNGDVRITDTRTLDFSGDFHFVYWDLSARGSEGIDVLGASGPA
                     WGDPGTTVPYEPSQFPSVGAWTGETGTYGIRDAGGVVTVQLNFNVTDATAAFTIEYVA
                     RGAARRWSDTAELYWQFIGQDVAVESRDVSVTVRLPQGVTDEQVRAWAHGPLWGTVTI
                     QPDASVVMKVDPLPANTFVEGRILFPAAALSAASASSGPRLAAVLAEEQRLADEANRS
                     RLWARLQAGLWGVLGVGVPLVALGLVMVLYFRYGREPRTQFDAQYLRDVPQPQLPPAL
                     AAFIWRMGSVGSDDVTATLLDLVNRKVIDLERVTIHEDRLFGPDETSTYKLTLRDERL
                     SDLLEHERQLCTFLFHEIAGGDELVLSELKDLAKTQRTAFAQGFQTWKATVQEEGERR
                     GYLDPHADRMAFIASAVAIVAIVAAGVAAVFSGFWWFLAGIPVGIVLIWVARAVKRRS
                     QEAAELHAQYAALERYLKDFGRLDEKPPDAIVLWEQFLVFAVIFGIADEVAKAMTIKV
                     PEVVSDPAFRTPYILWWGMPGEGGGLSAFSEMHQSFSQAVSVATSSSSSGSGGGGGFS
                     GGGGGGGGGGGFGAG"
     gene            complement(10241..10840)
                     /locus_tag="VFZ86_13545"
     CDS             complement(10241..10840)
                     /locus_tag="VFZ86_13545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643341.1"
                     /translation="MRAGLQLLSARSVKWLDAGVALWVVVWVILGVLIWSDIGAQAQL
                     STDVIKIGAAVRSTGEALGVVGGLPLVGGGIGDFADRIETMGGEVEASGQESRGGIER
                     TAAVAGLFVGIMPAALVLLLYLPARIRWRRYVGAIAAALAGGAGDPAFEQYLARRAVD
                     ALPWDQLRAITPDPWGDIRRGACRALADAELERLGLRRP"
     gene            10967..11983
                     /locus_tag="VFZ86_13550"
     CDS             10967..11983
                     /locus_tag="VFZ86_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HEX5643342.1"
                     /translation="MTTPKEAGFRMPPEWTPHAGCWMAWPCRPENWDDIEAARATYVE
                     VARVISRFEPVTMTANTEDAGAARRALTGVLGADVVVVPSDDSWARDTAPTFVTDGRG
                     ALGGVDWRFNAYGGIYEDYGRTQRMARRILDLVGARRFAAPLVLEGGAAHVDGEGTVL
                     TTTEVVLDPRRNPGLERADAERLLCEYLGAEKVLWLTAALDHDNTGGHVDNLACFAAP
                     GVVLALGCADRDDPQHAAIQENLVRLRAATDARGRPLEVVELPLPARPESRGRRLSPS
                     YVNFYVANGGVVMPAFGDPADAAASAVLARLFPRRAVVPVSTLELAKADGNVHCITQQ
                     QPVA"
     gene            complement(12020..12622)
                     /locus_tag="VFZ86_13555"
     CDS             complement(12020..12622)
                     /locus_tag="VFZ86_13555"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5643343.1"
                     /translation="MSGAAPAATSVPEPGRAEAQAARFFTNLLLSPLYRHYVEQMGLR
                     GDEHVLDYGSGSGAAARHLARLLETGGGRLTCMDVSARWQAVLRKVLRAYPDVEYLLG
                     DVRAMSLPAARFDVVLVHWMLHDVPPRDRPAIVAELARLLRPGGRLFLREPTGKKHGM
                     PAARARELSAAAGLIETRTREGRIFLLGEYYRAVWTKPAG"
     gene            complement(12624..12770)
                     /locus_tag="VFZ86_13560"
     CDS             complement(12624..12770)
                     /locus_tag="VFZ86_13560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643344.1"
                     /translation="MAIVVAVCNWLNAPTWVMWGLPIVICGGTAVGWAVRAVKWAGRR
                     PGTT"
     gene            12894..13550
                     /locus_tag="VFZ86_13565"
     CDS             12894..13550
                     /locus_tag="VFZ86_13565"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX5643345.1"
                     /translation="MLIAFLGDSLTEGWPGAAYFPLLDRRLWQHRLLNRGRAGDTVAD
                     LLTRMRYQGLERVDCAFIWVGANDAVMAAWDSSELGSGWSWPERLMRLAGDYEELLEW
                     TEARAPCIVVVRPLVLESEGSIWEERAAEVAEAIARIAAARGSCRVVDLRPAFAAAAG
                     KDDGPFTTDGVHFTDAGARVVADVFAAVVTELEAEGAPSGARDDGPPEYAAGDPGDGA
                     "
     gene            13550..14230
                     /locus_tag="VFZ86_13570"
     CDS             13550..14230
                     /locus_tag="VFZ86_13570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643346.1"
                     /translation="MKRLHKVALAAVALEALGAAYSFILLPRLRRWGATGDEVSRELP
                     GDALVAAPLYTTTHAITVRAAAEKVWPWLVQIGQNRGGFYTYDALENLAGLKVRSADR
                     VHPEWQDLRAGEDYLTLDPDETMKMTIAVLEPPRAFVVRSGAPGEPPQGAGSLFRGEM
                     AWTWGFYLEPAGPAESRLIVRSRASWRRTVPAAFAQAFGLEPAHFIMEEGMLRGIRDR
                     AQRAAAGA"
     gene            14243..15142
                     /locus_tag="VFZ86_13575"
     CDS             14243..15142
                     /locus_tag="VFZ86_13575"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5643347.1"
                     /translation="MPDFIWEGHRLHYREAGVAGAPLLIVLPGSTASSASHAGELAYF
                     GRTHRVAALDYLGTGASERLSPWPLDWWENGAAQATALAARLGAERFSLLGCSGGGTA
                     ALLTAADHPHRVTAVVADSCPEKLTPEELRGSVAGRTGVPAVGQEPSGDAGASGPPAG
                     AGISEQPFPGSIVEMLWREGPRLLRKLVTPSQRAFWKLAHGDDWAEVVAADAALLLEL
                     ADRGGWDPLAGRLGDVRCPVLLTSSLGDQELPSVVAQHQHLAMELPDCRLWVTPRGGH
                     PAMWTDKRGFRREAARFLAGVKL"
     gene            15394..18129
                     /gene="fdhF"
                     /locus_tag="VFZ86_13580"
     CDS             15394..18129
                     /gene="fdhF"
                     /locus_tag="VFZ86_13580"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015758916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:16471..16473,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HEX5643348.1"
                     /translation="MTGPAGAETATIQATIDGREVTVPAGTTILAAARDLGIDIPTLC
                     HHETLSPAASCRLCVVEVEGRANLLPACATELTDGMVLQTESADVVEARKLILDLLLS
                     DHPMGCITCEQSGSCTLQDYCYRYGIEHSTFVGDKRELPIESDNPMIERDMNKCILCG
                     KCVAVCGEVQVTGAIDFTGRGFDSQIAASFDKPLDTDFCRFCGQCADICPVGAISNKQ
                     LKGVRTWEREKVRTTCPFCGVGCNFDLNVAGGKIVGVTAAYDAPANQGSLCVKGRFHT
                     DMIYSPDRITTPMIKKDGVWENATWDEALDLVAERLLEIRGEHGSLSVGVLSSARCTN
                     EENYLLQRLTRAGLNTNSIDHCARTUHAPTVAGLATTLGSGAMTNSIGDIVGNDVLFI
                     IGSNATEAHPIIGNKMKQAHLRGAKLIVVDPRRTELAEHAHLWLRLKPGTDNALVNGI
                     LHTIISNGWHAKDYIDARTEGFDDLWATVKDYPAERAAEITGVPAGTIYAAAELYAKT
                     PKAGIFYTLGITEHTVGTANVMNLANLGMVTGHIGFPNAGVNPMRGQNNVQGSCDMGA
                     LPNSFPGYPSVGDEAAKARFAEAYGVPMPDQMGLRIPEMIELAANGTLKAMFIMGEDP
                     ALTDADANHVRKALGNLEFLVAQNIFMTETAKYADVFLPAALYAEKDGTFTNTERRVQ
                     RVRKAVEPPGDTRPDWQIIQDLGRRMGLDWHYDSPEDVFEEIRTLVPQYAGMTYERLG
                     TQGLQWPCPTEDHPGTPVLHVGTFTRGKGLLQGIPFQPPAELTDDEYPMLLTTGRMLY
                     HYNIMTRLSQNLEWLRPYELAELNPADAERLGVVEDELVRVSSRRGSITTRVTLTERV
                     PPGTMFMTFHFKESPVNELTNAAGDPVTMTAEFKVCAVRMDKEPAAAESHGAAAR"
     gene            complement(18231..18944)
                     /locus_tag="VFZ86_13585"
     CDS             complement(18231..18944)
                     /locus_tag="VFZ86_13585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643349.1"
                     /translation="MTTASPDAATSGRALGRCLIATAAAYLFVVLVMTAVTALGAPQG
                     DTVVAQVADLSRDDTVYRWGFFFASLLPAAMVPFMAVVALGVVAPAEGEGITEARLCS
                     WAGIALVAAYAPLSAAAYASQYTVFDWLLRRDLAAAAPWYFNNVHGGPYTFDLLAYAI
                     WGLGAIIIAWPLLRRPGALHWMSWTLVASGLLSMAAFGLHALESDLASAATLTSAVLT
                     VPLALAAIVHGRGRLRRPA"
     gene            19263..>19733
                     /locus_tag="VFZ86_13590"
     CDS             19263..>19733
                     /locus_tag="VFZ86_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5643350.1"
                     /translation="MRTTTISEREGRDQASETLARLEREFTGESLFTERGPGRAVVTA
                     RGLTRVYGKGDAQVRALAGVDIDITEGRLTAIMGPSGSGKSTLMHILAGLDQPTGGRV
                     SIDGVEITRLKEKKLTQLRRDKIGFIFQSFNLLATLTADENIVLPLSLARRRADE"
BASE COUNT         2854 a         7263 c         6945 g         2671 t
ORIGIN      
        1 cagacaacgg acgggcttgt cgggaggtcg cgctccgggt aacatatgaa cacctgttca
       61 tatgttcaga ggagagacga tggccgatcg tcagctcgaa gcgcgggtga tctgcggtaa
      121 ggacgacgag gacctgctcg accactgcga cgtgcacgtc gtcgacccgg agcgggtcac
      181 cgcggtgcgc gagcggctgg tctcgcccgc ggtgggcgaa cagatggcca acgtcttcaa
      241 ggtactcgcc gaccagagcc gctgccggct catggccgcc atcatcgaag ccggcgagct
      301 ctgcgtctgc gacctcgccg ccaccgtgga catgagcgag agcaacgtga gccaccacct
      361 ccgcgtcttg cgtgccaacg ggctcgtccg cgcgcgccgc gccggcaaga tggtcttcta
      421 ctccccggac gacgcgcaca tccgcctgct tctcgacatc acgcgcgagc acgtggaaca
      481 cggccccgag gacctctccg gcgaggcggt cgtcagcggg cggcgcgagc ccgctacggc
      541 gccgggacgg tgacgcgatg accgcgcgca ccatcgagct ctcgctgctg ctgccgcggg
      601 cggcggagtg cggcggttgc gtgaccgagg tcggccgcga gctgctgcgg ctcgacggca
      661 tccacgccgt cgagccggac gtccctcgcg gcctcctgcg cctcacctat gacgaagcgg
      721 ccgtgaccgg cgacgacgtg gagaccttcg cccgccgcgc cggagcgcag gcccactgtg
      781 cggaccactg cccgctcgcc gtgcacgagc acggccccct cgacctctcg caggcgctgg
      841 ccgtggagga tcacggcgaa cgccgcatcc tccacgtcac cggcatggac tgcgccgact
      901 gcgcggtgaa gctgcagggc gcgctgcgcc gggagcgcgg cgtgagcagc gcggacgtca
      961 acttcggcgc ggccaccctg gcgatcgcca tcgacccctc gcagacggcc ctgccggacg
     1021 tcttccgcgc cgtccgccgg ctcgggtacg acaccgtgga gcggcgcggc gcggaagagg
     1081 atccaggggc gcgccggggc gcagccgcga cggccgggcg cggcttctgg ctctcggagc
     1141 cgcgcgccat cgccacgctc atgtccggcg cgctcaccgt cgccggcttc gtcgccacgg
     1201 cggccgcgcc ggaggcggcg ccgtggctct tcggcgccgc catgctcagc ggtggcctgt
     1261 acgtcgcccg cgccgccgtg ttcagcctgc gcggccggca ggtcgacatg aacgtgctca
     1321 tgacgctggc catgatcgga gccgcggcca tcggcgagtg gtccgaggcg gccctggtcg
     1381 ccttcctctt cagcctcggc acggtgctgc aggtggccac gctggagcgc acccgccgcg
     1441 ccatcagcgg cctcatgaag ctggcgcctc cgaccgccgc cgtcctgcgg ccggcggaga
     1501 cgggcgccgc cctgcgggag gcgaaggtcg acgtcgccga catcgtcgtc ggcgacgtcg
     1561 tcctgatccg ccccggtgag cgtgccgccg tcgacggcgt cgtcgtcgag ggcgcggccg
     1621 ccgcggacca ggcgccggtg accggcgagt cggcccccgt cgccaaggga cccggcgacc
     1681 aggtcttcgc aggctcgatc gtcgagggcg gaacgctgcg cgtgcgcgcc acggcggcgg
     1741 cctccgacac gaccatcgcc aagatcgtcc acatggtgga ggaggcccag gcgcagcggg
     1801 cgccggcgga gagcatcgtc gaccgcttcg ccggccggta cacgccggtc gtcgtggcgc
     1861 tggcggcggc ggtcgccttc gtgccgccgc tcttcggcgc ctcgttcgac acctggttct
     1921 accgcggcct cgcgctgctc atcatcagct gcccctgcgc gcttgtcatc tccaccccgg
     1981 tcagcatcct ggcggcgctg gcgcgtgcga cgaagaacgg tgtgctgatc aagggcggcg
     2041 cctacctgga gcagatggcg ggcctcaagg cggtggcgtt cgacaagacc gggacgctca
     2101 cgctcgggcg cccgcaggtc acggacgtgg tgccgctcca cggcacgtcg ccggaccggc
     2161 tgctgacggt tgccgccgcg ctggagcggt acagcgagca ccccctggcg cgggcgatca
     2221 cagagcgggc cgcgcaggac ggatccgccc aggcggtcgc cgcggcggcg cccccggcgg
     2281 aagtcgccgc ggctgcctgc tcctgctccg agcccggctg ctgcacggcg ggcgcggatg
     2341 ccgcccaggt cgcggacggc gacgccgctc gcggccacgc accgcatgac gcggcggacg
     2401 cgcacctcca ccggggacca gacgtggagc ggttccgcgc gatccccggg cgcggggtgc
     2461 gcgccgagct ggacggccgg ctgcacttcg ccggccggcc ggacctcctc ggcgcgcacg
     2521 ccttcgaccc ggcgttgacg gccgcggtct cgcgactcga gcgtgagggc aagaccgcgg
     2581 tcgtcgtcgg cgacgaggag ggcccgctcg gcgtcgtcgc cgtcagcgac ccgctgcgtc
     2641 ccggggccca cgaggcggtc gcagcgcttc gcgacctggg ggtcgccgag atcgcgatgc
     2701 tcacgggaga caacccggag acggctcgtg cggtggccgc gcaggccggc gtcacggacg
     2761 tgcgcgccgg cctgctgccg gcgcagaagg tcgacgccgt cgtggagctc cgcgaccgcg
     2821 ccggcgcggt cgcgatgatc ggcgacggcg tcaacgacgc gccggcactg gcggcggcgt
     2881 ccctcggcgt cgccatgggc gccgccggca ccgacgccgc cctggagacc gccgacatcg
     2941 ccctgatggg cgacgacctc tcggcggtcc cggagaccat ccgcctggcg cgccggacca
     3001 cccgcgtcat ctggcagaac atcgtcttct ccatcgccgt caaggcggtc ttcctggtcc
     3061 tcgcgccact gggcctggtc agcctgtggc tggccgtctt cgccgacatg ggcacgtcgc
     3121 ttctcgtcac cgccaacggg ctgcgcctct accgccgctg acggggcaga cacgccgccg
     3181 gcggagggct agaatcagcg gcaggagccg accgccggag cccggctcga ggaggcgcga
     3241 tgaccaccat catctacacc aagcccggct gcccgtactg cgccgcggcc aagcaggacc
     3301 tcgatgcccg gggcatcacc tacgacgaga tcgacgtcta cacgaccccg ggcgcccgcg
     3361 accagctcgc cgagctcacg ggcggcatga acgtcgtgcc ggtcgtgatc gaggaggacg
     3421 gcgaggtgcg cgtcgccgcc ggaggtggct gaggcatctg acgggcgcgc gtgacgcgcc
     3481 cctcgccgcc tacttcttga ccggcaggcc ggccttcttc caggccatca ggccgccgtg
     3541 cacgttggtc accctggtga agcccatctc ggcgagcttc ctcgcctgcc gcggactgcg
     3601 gtgcccgctc aggcagatga cgaggacgtc gtcgtcacgc cccagccccg tcctgtcctt
     3661 gctgatgagc tggggcggga cgctgatcgc gcccttgggg tgaccggagt gcgcgtactc
     3721 acgcgcggag cggacgtcga tcagtctggc gccacgcttg gagcgcgcga acgcctgcct
     3781 cacgtcgatc gtcggcaccg ccacgggcgc cgtcgcggcg ccctcgggcc tcttcttgaa
     3841 cggccacatc ctcgggcggc gttcagaccg cggcgccgtc gtgctggggc tcctccacga
     3901 cggcgtcgta gaggacctcg aacggcccgc tggtcaggat cgactgcagc gtgccgtaca
     3961 cgagctgcac ctgcgcgccc tggtagaagt cgtcgtgagc ctgcagagag tcccactccg
     4021 tctcgaagac gaagtggacg tcgccgaggg tcgcctcgcg accttcatcg cgcagcgcgc
     4081 tgccgagcgg cgacatgccg aacgaccgca ggacccgatg gctggtgtgt cccgccgtcc
     4141 cctggaggag tccggcgagc tcctgaagcg cgcgctcggc acggtgctcg gtgccgtggc
     4201 ggacgctgaa catggtctga ccgatcaaca tcgcactcac tccctgtcgg ggacttgcat
     4261 agcttgtgta cccgtctggg cggcgatgga acgatgcagg tcagcgcacc acctggcgga
     4321 aggccgcgag cagggccgcc gtggtgagcg ccgcggcgcc gtcgccggtc cagacgatct
     4381 tgccggcgcg gtcgatgatg aatgccatgg ggcgcgccgt gccgtccgcg cgcagcgcgc
     4441 cgtactgccg ggcgacgctg ccgtcgtcgg gcagcagcgg gaacgccagg ccggggacgc
     4501 cggcggcgag agtcttgcac tcgtcgcggc cggcgtcgca gatgccgacg atctggccgc
     4561 ggagccccca caccgcatcc gccgtctcgc tcagctgctc gagcagcccg gaggcgccgg
     4621 gcgaccgcgt cgggatgaac accagcaccg ggaaccagtc gccgcggtac tggttgagct
     4681 tgatctcgct tccgtcgacg gcgctgagag tgaacgtcgg cgcttcacgg ccgacaccga
     4741 agtcgtaccc cacgtgcgga cctcctgacg gtgccgcggg cggcgcccgc gcgggtcaag
     4801 tcgcgagtgt agcaaagatg ccggcgtgag gcccctgcac gacaggggcc tcacgccgtc
     4861 cagaactgcg cgaccgctcg cccggcggcc ggtggagccc gggcgccggg caaggtgatc
     4921 gcagcctcgc gggggccgcc gggcgcggcg gcgaggggca ggcccgcgga catcgccggc
     4981 gccgtctccg gggacggcgg cgtggcggcg tcgtgcgccc gcagcctcgt gccgcggtgc
     5041 gacgtcgcga ctgcgccggc gacctccttc ctggtcgcgc tcgcctggac ggcggcgagc
     5101 ggcgcgaccc gggcgccggg gggaggcggc cctacgcgcg ggccgtcggc ggcggcggcc
     5161 gtcacgacga gcagggtgac cggggcggcg acggcgaaga cgacggctgc tccgcgcacc
     5221 tctcgcacct cctctgcttg ccgacgggat cttcgccgtg agtctgccac ccgctccttg
     5281 gtcccggctg agggtggtct gagcccggac tgtgagttcg ggcgcggtgc gggcggcgct
     5341 caggttgctc tcagacggac ctcaggccac actcaggggg tccggctact ctgatgcgcg
     5401 cggtcccgtg cgctcgtcgc cggctccgca cccgagcgca cgacgagaga ggatcacgaa
     5461 tgagacgcgt caccaccgtc ctgatcgccg ccctggcgct gctcggcgtc gccgccgcca
     5521 gcctgaccgt cgcggcgtgc ggcggcgaaa gcgtcccaag caacgccgtc gccaccgtcg
     5581 gcgatgtcac cgtgaccaag gccgacttcc aggacctgct cacccaggca caggtacaga
     5641 tgaaggcgca aggcatgaac gtgcccaagg agggcagcgc cgcctacgac cactacgtcg
     5701 ctcaggtcgt caactacctg gtgcaggagc aggtcgtcga gcagagcgcc gaggaactcg
     5761 gcgtctcgat caccgacaag gaggtcggcg agcaggtcgc ggagctcgtg caggcctacg
     5821 gcggcgagca gaaggtcgtg gacctcctcg cggagcaggg catgaccatg gaactgctca
     5881 agcgctccat ccgcagccag accctgtccg acagggctgc cgagatcgtc accgaggagg
     5941 cgtcggtgag cgacgccgag atccagatgt actgggaggc gcacaaggcc gagctcagca
     6001 agaagaagaa gacggcgacg ctggccaagg ccaggaccac catcgagcag atcctggtct
     6061 cggccaagaa gctgcagctc tggaacgcat gggtcgccga gcgcgccgag gcactggggg
     6121 tcgagtacgc gtccgggtac gaccccactc tgctgacgcc gtcggcgtcc gcctcgccgg
     6181 cggactgaac gccgcgccgc gcctcagcgc ggcgggcggc gggcggcggc tgcagctacc
     6241 gtacgcggac ggggccggcc cgccgtagcg gcggccggcc ccgtccgggt cctgcggatc
     6301 tcgcgtccgg ctcagaagct cttgtagagc tccttgccgg cggcgcagta ggggcacttt
     6361 tccggcacgt cgccgatcac tgtgtgaccg cagacgaggc agacgctcac cggctggtcg
     6421 gagatgtcct tgcccgcgag cacctgctcc cgggcgtcgg tgtactgacc cttgtggtcc
     6481 acctcggact tgagcgcgta gttgatgctg cgcacggcct ggttgttgcc ctgcagcttg
     6541 gcgatcccgt gataggcggg gtacatctcg tcgacctcga agctctcgcc tccgaacgcc
     6601 gccgccaggt tgtcggcggt gctcccgatg ccgcccatca cccgcaggtg gttgcgcgcg
     6661 tggcgcgtct cggcgaagga gatggcgcgg aacagcttgg cgacgttcgg atagccctct
     6721 tcatcggcct ggtcggccca cacgaggtac ttctcggccg cctggctctc gccggcaaag
     6781 gcggccgcga ggctctcctt cgtcatcgcg ctgagctctt cggctttggg aaggcgcttc
     6841 tccatggctt cgtccatcag aactccttga agaactcgct ggtggtgccg cactgcgtgc
     6901 agtaggccgg ggccttgtca ccgaccaccg tgtgcccaca gaccggacag acgaacacgg
     6961 gtgtcgtgtc gaggtccttc tcgccctcga cacccttgag cgtggtgtcg tagatgccct
     7021 ggtggtccat ctcggccttc agggcgtagc ggatcgagac ctgggcgctc tcgttctcct
     7081 gcagcttcgc caccgcgtcg tacgccgggt acatctcgtc gatctcgaag gtctcgccac
     7141 cccaggccgc cttgaggttc tcggcggtgg tgccgatgcc gccgagtacg ctcaggtggt
     7201 tgcgggcatg cgtgctctcc gcgtacgcca cggcgcggaa caggcgtgcg acgttcgggt
     7261 agccctcggc gtcggcgacg tcggcgaagg cgaggtactt ctgcgaggcc tggctctcac
     7321 cggccagcgc ggcggcgatg ttggccgccg tcatgtcgcc cttggcttcc ggggcgggcg
     7381 gggtgttctt gaagggttcg gccatcgtgt cgtgctcctc cttgtcggcg tagggcgcag
     7441 aacggcgaga ggccgcgcgc ccggttccat gggtcggtag cggaccccta tgctaacagc
     7501 gtcgtcggcg gtcgccgttt ggtggcggga aggctcggac cggcagtgcg cgaggcgcgt
     7561 gacggccgcg cgcccgctct cgcggggcgc ggccgccggg ctgccggaag gcaggtcgtg
     7621 gactgtagcg tcgaatcgcg gcgcactgtg aatggctcgc cgctcaggcg gcggccgcgt
     7681 gcggctccag ggcggggacg agccggtgca gtgctgtatg gacgcggctc gggctcgcgc
     7741 ggtcgaacaa cgtgtagtag acgtccgggc gtgactggct gcgcgtgaac tgggacagat
     7801 ccacggtgag gcctgcagcc cgcgcctcac gggcctgctc gctgtcgatc gcgaggagcg
     7861 cggccgtcgc cggcacgctc gcgtcgctgc agagttcggc gatgtcctgc ggcaggctcc
     7921 agagcgagaa ggcgagcggt gtgccgccgg cctcgctgag cacgagtcgc accccgagct
     7981 ccacctctcc ggtgaagtcg aagtgacggc ggtcggcgcc gcgcgcgacc gtgaccgcct
     8041 tctgcacgcg ccggtatttc tcgccgaacg cggcggggat tggacgtgcc atggtgaccc
     8101 tcctggtgca ctccccgtaa gccttccgga gccctggggg atgtcgctct ctttcgcgca
     8161 gcatcttccc ggcagaagtc gccgataaac accctgcaga gagtgaaaac ggtgccgcgc
     8221 cgagcagcca gcgcgcaggg gccggcgggt cagccggcgc cgaagccgcc gcccccgccg
     8281 ccgcccccgc cgccgccgga gaacccgccg ccgccgccgg agccggacga ggacgacgac
     8341 gtcgccaccg acaccgcctg gctgaagctc tggtgcatct cgctgaacgc cgagagcccg
     8401 ccgccttcac ccggcatccc ccaccagagg atgtacggcg tgcggaaggc cgggtcactc
     8461 acgacctcgg gcaccttgat ggtcattgcc ttcgccacct cgtcggcgat gccgaagatc
     8521 acggcgaaga cgaggaactg ctcccagaga acgatcgcat ccggcggctt ctcgtcgagc
     8581 cttccgaagt ccttgaggta ccgctcgagg gccgcgtact gcgcgtgcag ctccgcggcc
     8641 tcctgcgagc ggcgcttgac cgccctcgcc acccagatca ggacgatgcc cacggggatg
     8701 cctgccagga accaccagaa cccgctgaag acggccgcca cacccgcggc gacgatggcc
     8761 acgatggcaa cggccgacgc gatgaaggcc atgcggtcgg catgcgggtc gaggtagccg
     8821 cgtctctcgc cctcctcctg caccgtcgcc ttccaggtct ggaagccctg ggcgaacgcc
     8881 gtgcgctgcg tcttggcgag gtccttgagc tcgctcagca ccagttcgtc gccgccggcg
     8941 atctcgtgga agaggaaggt gcagagctgc cgctcgtgct cgagcaggtc cgagaggcgc
     9001 tcgtcgcgca acgtcagctt gtacgtggac gtctcgtcgg ggccgaagag cctgtcctca
     9061 tgaatcgtca cccgctcgag gtcgatcacc ttgcggttca ccaggtcgag aagagtcgcg
     9121 gtgacgtcgt cgctgcccac gctgcccatg cgccagatga acgccgcgag ggcaggcggg
     9181 agctgcggct ggggcacgtc gcgcaggtac tgcgcgtcga actgcgtcct gggctcgcgt
     9241 ccgtagcgga agtacaggac catgacgagg ccgagcgcga ccaacggcac gcccacgccc
     9301 aggacgcccc agagccctgc ctggaggcgt gcccagaggc gcgagcggtt ggcttcgtcg
     9361 gccagccgct gctcctcggc gagcaccgcg gccagccttg ggccactgct cgcggaggct
     9421 gcgctgaggg ccgcggccgg gaacaggatg cggccctcga cgaacgtgtt ggccgggagc
     9481 gggtcgacct tcatgaccac gctcgcgtcc ggctggatcg tgacggtgcc ccacagcggc
     9541 ccgtgtgccc aggcgcgaac ctgttcgtcg gtcacaccct gcggcaggcg gacggtgacg
     9601 ctgacgtccc gcgactccac ggccacgtcc tggccgatga actgccagta gagctccgcg
     9661 gtgtcgctcc agcgcctggc cgccccccgg gccacgtact cgatcgtgaa cgcggcggtc
     9721 gcgtcagtga cgttgaagtt gagctggacc gtcaccaccc cgcccgcgtc tcggatgccg
     9781 tacgtgccgg tctcccccgt ccacgcgccc acggacggga actgcgaggg ctcgtagggg
     9841 accgtcgtcc cggggtcgcc ccatgcgggc cccgaggctc cgagcacgtc gatgccctcg
     9901 gagcctctgg cggagaggtc ccagtacacg aagtggaagt cgccggagaa gtccagcgtc
     9961 cgcgtgtcgg tgatgcgcac gtcgccgttg cgcttgacgg tcgcgtcgac cgacacgctg
    10021 gtgatggaga agtccttggc cgccgcgggt ccggccgcca ggccggcagc tccggcggcc
    10081 accagcagca gcacgccgag cgccggcagg acacggcgga cgcgggcggc aagccgccgg
    10141 cgctcccccc tgccgaggaa tccgttcatg acggcgactt taccatccgt cgtcgccgag
    10201 aggccgggcc ggtcgccccc gcggcgggcc ggcgacgccc tcatgggcgg cgcagaccga
    10261 gccgctccag ctccgcgtcg gccagcgcgc ggcatgcgcc acggcggatg tcgccccagg
    10321 gatccggcgt gatggcgcgc agctggtccc aaggcagcgc gtcgacggcc cggcgggcca
    10381 ggtactgttc gaacgccggg tcgccggcgc ctcccgccag cgccgccgcg atcgcgccca
    10441 cgtaccgcct ccagcggatc cgcgccggca ggtagagcag gagcacgagc gccgccggca
    10501 tgatgccgac gaacaggccg gcgaccgccg ccgtgcgctc gatgccgccg cgactctcct
    10561 gcccgctcgc ctctacctcg ccgcccatgg tctcgatgcg atcggcgaag tccccgatgc
    10621 cgccgcccac caggggcagg ccgccgacga ccccgagcgc ctcgccggtg gacctgaccg
    10681 ccgcgccgat cttgatcacg tccgtgctca gctgcgcctg cgcgccgatg tcgctccaga
    10741 tgagcacgcc gaggatgacc cacaccacga cccagagggc gacgcccgcg tcgagccact
    10801 tcactgagcg ggcgctgagg agctgcaatc cggcgcgcat ggtccctccc ggtcgacccg
    10861 cctactctac cccgcggcga gagaccggct gctcgcacgg cggcgcgccg gcgctgcggt
    10921 atgctcgccg cgacccggag gtgaccgtgg cgaccgagcg ccccagatga cgactccaaa
    10981 ggaagccggc tttcgcatgc cgcccgagtg gacgcctcac gccggctgct ggatggcgtg
    11041 gccgtgccgg ccggagaact gggacgacat cgaggcagcg cgcgcgacgt acgtcgaggt
    11101 cgcgcgcgtg atcagccggt tcgagcccgt cacgatgacc gccaacacgg aggacgccgg
    11161 ggccgcgcgg cgcgcgctca ccggcgtcct cggcgcggac gtcgtcgtcg tccccagcga
    11221 cgactcgtgg gcccgcgaca ccgcgcctac cttcgtcacc gacggccgcg gcgcgctcgg
    11281 cggcgtggac tggcgcttca acgcctacgg cggcatctac gaggactacg gccgcaccca
    11341 aaggatggcg cggcgcatcc tcgacctggt gggggcgcgg cgcttcgccg cgcccctcgt
    11401 cctcgagggc ggcgcagcac acgtcgacgg cgagggcacg gtgctgacga cgaccgaggt
    11461 cgtgctggat ccacgccgca accccggcct ggagcgcgcc gacgccgagc ggctgctctg
    11521 cgagtacctc ggcgccgaga aggtgctgtg gctcaccgcg gcgctcgacc acgacaacac
    11581 cgggggccac gtggacaacc tcgcctgctt cgccgcgccc ggggtcgtcc tggcgctcgg
    11641 ctgcgccgac cgcgacgacc cgcagcacgc ggcgatccag gagaacctcg tgcggctgcg
    11701 ggcggcgacc gacgcccgcg gccgcccgct cgaggtggtg gagttgccgc tccccgcgcg
    11761 gccggagtcc cgtgggcggc ggctctcgcc cagctacgtg aacttctacg tcgccaacgg
    11821 cggcgtcgtc atgcccgcct tcggcgaccc ggcggacgcc gcggcgagcg ccgtcctggc
    11881 ccggctcttc ccccggcgcg cggtggtccc ggtctcgacg ctcgaactgg ccaaggccga
    11941 cggcaacgtc cactgcatca cgcagcagca acccgtcgcc tgacgcagtc gaagccgccg
    12001 gtccgcagcc gtctcggggt cagcctgccg gtttggtcca gaccgcgcgg tagtactcgc
    12061 cgagcaggaa gatcctgccc tcgcgcgtcc gcgtctcgat gaggccggcg gcggcggaca
    12121 gctcgcgcgc ccgcgccgcc ggcatcccgt gcttcttgcc cgtaggctcg cgcaggaaca
    12181 ggcgcccgcc cgggcgcagc agtcgcgcga gctcggcgac gatcgccggc ctgtcccgcg
    12241 gcgggacgtc gtgcagcatc cagtggacga gcacgacgtc gaagcgcgcg gccggcaggc
    12301 tcatcgcgcg cacgtcgccg aggaggtact cgacatccgg gtacgcgcgc agcaccttgc
    12361 ggaggaccgc ctgccagcgc gcggagacgt ccatgcaggt gaggcgcccg ccgcccgtct
    12421 cgagcagcct cgcgaggtgc cgggcggcgg ccccgctccc gctcccgtag tcgagcacgt
    12481 gctcgtcgcc gcgcaggccc atctgctcca cgtaatgacg atacagcggg ctcagcagca
    12541 ggttggtgaa gaaccgggcc gcctgcgctt cggcgcggcc cggctcgggg acgctcgtcg
    12601 ccgccggcgc ggcgccactc acctcacgtc gtccctgggc gcctgccggc ccacttgacg
    12661 gcgcgcaccg cccagccgac cgcggtgccg ccgcagatga cgatcggcag tccccacatg
    12721 acccacgtcg gagcgttcag ccagttgcac acggcaacca cgatcgccac tcagacgacg
    12781 aggccgacga tgtccaggat cgccttcttc atcactctcc cctccgtggt cggaccactc
    12841 cttccccttt cggcagcgta ccccaggagc ccgcgagcgc atagactggt cgcatgctga
    12901 tcgccttcct cggagacagc ctgacggagg ggtggccggg cgccgcctac ttcccgctgc
    12961 tcgaccggcg cctttggcag caccggctgc tcaaccgggg gcgggcgggc gacacggtcg
    13021 cggatcttct cacccggatg aggtaccagg ggctcgagcg cgtcgactgc gccttcatct
    13081 gggtcggcgc caacgacgcg gtcatggcgg cgtgggactc gtccgagctc gggagcggct
    13141 ggagctggcc cgaacgcctc atgcgcctcg ccggcgacta cgaggagctg ctcgagtgga
    13201 cggaggcgcg ggcgccgtgc atcgtcgtgg tgcgcccgct cgtcctcgaa tcggagggct
    13261 ccatctggga ggagcgcgcg gccgaggtcg ccgaggcgat cgcacgcatc gcggcagcac
    13321 gcggctcctg ccgagtcgtc gacctgcggc cggcgttcgc ggcggcggcc gggaaggacg
    13381 acggcccctt caccacggac ggcgtgcact tcacggacgc cggagcgagg gtggtggccg
    13441 acgtgttcgc cgccgtcgtc accgagctcg aggccgaggg tgcgccatcc ggcgcgaggg
    13501 acgacggacc gccggagtac gcagccggcg atcccggcga tggggcctga tgaaacgcct
    13561 gcacaaggtc gccttggccg ccgtcgccct cgaggcgctc ggcgcggcct actccttcat
    13621 cctgctgccg cgcctgcggc gctggggcgc gaccggcgac gaggtgtcgc gcgaactgcc
    13681 cggcgacgcg ctggtcgcgg cgccgctgta cacgaccacc cacgccatca cggtccgcgc
    13741 cgcggcggag aaggtctggc cttggctcgt gcagatcggc cagaaccgcg gcggcttcta
    13801 cacctacgac gccctcgaga acctggccgg gctcaaggtg cggagcgccg accgcgtcca
    13861 tcccgagtgg caggacctcc gtgccggcga ggactatctg acgctcgacc ccgacgagac
    13921 catgaagatg accatcgccg tgctcgagcc gccgcgggcg ttcgtcgtgc gcagtggcgc
    13981 cccgggagag ccaccgcagg gcgccggcag cctgttccgc ggcgagatgg cctggacctg
    14041 gggcttctat ctggagccgg ccggcccggc ggagagccgg ctcatcgtcc gctcgcgcgc
    14101 ctcctggagg cgcaccgtgc cggcggcgtt cgcgcaggcg ttcggcctcg agccggcgca
    14161 cttcatcatg gaagagggca tgctccgcgg catccgcgac cgcgcgcagc gcgccgccgc
    14221 gggcgcctga gggcgaggac cgatgccgga cttcatctgg gaggggcatc gcctgcacta
    14281 ccgcgaggcg ggcgtcgccg gcgcgccgct cctcatcgtc ctccccggca gcaccgcttc
    14341 gtcggcgagt cacgccggtg agctcgcgta cttcggccgc acccatcgcg tcgccgccct
    14401 cgactatctc ggcacgggcg cgtccgaacg cctctcgccg tggccgctcg actggtggga
    14461 gaacggcgcc gcgcaggcga cggcgctcgc cgcgcgactc ggcgccgagc gcttctccct
    14521 cctcgggtgc agcggcggcg gcacggcggc gctgctgacc gccgcggacc atccgcaccg
    14581 ggtgaccgcc gtcgtcgcgg acagctgccc ggagaagctc acgccggagg aactgcgcgg
    14641 cagcgtcgcc gggcgcacgg gtgtgccggc ggtggggcag gagccctccg gcgacgccgg
    14701 cgcctccggg ccgccggccg gcgccggcat cagcgagcag ccgttccccg gctcgatcgt
    14761 cgagatgctc tggagggagg ggccgcggct gctgcgcaag ctcgtcaccc cctcccagcg
    14821 cgccttctgg aagctcgccc acggcgacga ttgggcggag gtcgtcgccg ccgacgccgc
    14881 cctgctcctc gagctggccg accgcggcgg ctgggacccg ctcgccggcc gcctcggcga
    14941 cgtgcgctgc ccggtgctgc tcacctcgag tctgggcgac caagagcttc cctccgtcgt
    15001 cgcccagcac cagcacctgg ccatggagct cccggactgc cgcctgtggg tgacgccgcg
    15061 cggcggccac cccgcgatgt ggaccgacaa gcgcggcttc cgccgcgagg ccgcgcgctt
    15121 ccttgccggc gtcaaactgt agctgggagc tacatccagc cggcgcccgc cgccgatcgc
    15181 gcgtcggcgc tcgccgcacg cctcgcgcgg gcgcgcccgg ctttcgcgcc cgcgccgccg
    15241 cgttgtacac tcgggctccc tccccgcgcc cggggaggac cgtccgtaaa ggcggcggcg
    15301 cgctcccctg acgcgcgcgg cgccgcagtg gaggctcggg ccgcgatggg agcggcccac
    15361 gtagctgcag tggagcaagc gaacggaggc ggcatgaccg gtcctgcagg cgcagagacg
    15421 gcgacgatcc aggccaccat cgacggccgc gaggtcacgg tgccggccgg caccacgatc
    15481 ctcgccgccg cccgggacct cggcatcgac atcccgaccc tgtgtcacca cgagacgctc
    15541 agcccggcgg cttcctgccg gctctgcgtg gtcgaggtgg agggccgggc caaccttctg
    15601 ccggcgtgcg ccaccgagct caccgacggc atggtgctcc agaccgagtc cgcggacgtg
    15661 gtcgaggcgc gcaagctgat cctcgacctc ctgctctcgg accaccccat gggctgcatc
    15721 acctgcgagc agtcgggctc ctgcaccctg caggactact gctaccgcta cggtatcgag
    15781 cacagcacgt tcgtgggcga caagcgcgag ctccccatcg agagcgacaa cccgatgatc
    15841 gagcgcgaca tgaacaagtg catcctctgc ggcaagtgcg ttgccgtgtg cggagaggta
    15901 caggtcaccg gcgcgatcga cttcaccggc cgcggcttcg actcgcagat cgcggcctcg
    15961 ttcgacaagc cgctggacac cgacttctgc cgcttctgcg gccagtgcgc cgacatctgc
    16021 cccgtcgggg cgatctccaa caagcagctc aagggcgtgc gcacctggga gcgcgagaag
    16081 gtgcgcacca cctgcccctt ctgcggcgtc ggctgcaact tcgatctcaa cgtcgccggc
    16141 ggcaagatcg tcggcgtcac cgccgcctac gacgcgccgg ccaaccaggg ctcgctgtgc
    16201 gtcaagggcc gcttccacac cgacatgatc tacagccctg accgcatcac caccccgatg
    16261 atcaagaagg acggggtgtg ggagaacgcg acctgggacg aggcactcga cttggtcgcc
    16321 gagcggctgc tcgagatccg cggcgagcac ggcagcctct ccgtcggcgt gctcagctcg
    16381 gcgcgctgca ccaacgagga gaactacctg ctgcagcgcc tcacgcgggc gggcctcaac
    16441 acgaactcca tagaccactg cgcccgtacc tgacacgctc ctacggtcgc cggtctggcg
    16501 accacgctgg gttcaggcgc gatgaccaac tcgatcggcg acatcgtcgg caacgacgtg
    16561 ctgttcatca tcgggtccaa cgccaccgag gcgcacccga tcatcggcaa caagatgaag
    16621 caggcgcacc tgcgcggcgc caagctcatc gtcgtcgacc cgcggcgcac cgagctcgcc
    16681 gagcacgccc acctctggct gcggctcaag ccgggcacgg acaacgcgct cgtcaacggc
    16741 atcctgcaca ccatcatcag caacggctgg cacgccaagg actacatcga cgcccgcacc
    16801 gagggcttcg acgacctctg ggccaccgtc aaggactacc cggcggagcg cgccgccgag
    16861 atcaccggcg tgccggccgg gaccatttat gcggcggcgg agctctacgc gaagactccc
    16921 aaggccggca tcttctacac cctcggcatc accgagcaca cggtcggcac cgccaacgtc
    16981 atgaacctcg ccaacctcgg catggtcaca ggccacatcg gcttccccaa cgccggcgtg
    17041 aaccccatgc gcgggcagaa caacgtgcag ggctcgtgcg acatgggcgc gctgcccaac
    17101 tccttcccgg gctaccccag cgtcggcgac gaggccgcca aggccaggtt cgccgaggcc
    17161 tacggcgtac cgatgccgga ccagatgggt ctgcgcatcc ccgagatgat cgagctcgcc
    17221 gcgaacggca cgctcaaggc gatgttcatc atgggcgagg atccggcgct caccgacgcc
    17281 gacgccaacc acgtgcgcaa ggcgctgggc aacctcgagt tcctcgtcgc acagaacatc
    17341 ttcatgaccg agacggccaa gtacgccgac gtgttcctcc ccgcagcgct ctacgcggag
    17401 aaggacggca ccttcaccaa caccgagcgc cgcgtgcagc gcgtgcgcaa ggccgtggag
    17461 ccaccgggcg acacgcggcc cgactggcag atcatccagg acctcggcag gcgcatgggt
    17521 ctcgactggc actacgactc tcccgaggac gtgttcgagg agatccgcac gctggtgccg
    17581 cagtacgcag gcatgaccta cgagcggctg ggcacgcagg gcctgcagtg gccgtgcccg
    17641 accgaggacc acccgggcac gccggtcctg cacgtgggaa ccttcacccg cggcaagggg
    17701 ctgctgcagg gcatcccgtt ccagcctccc gccgagctca ccgacgacga gtacccgatg
    17761 ctcctcacca ccgggcgcat gctctaccac tacaacatca tgacgcgcct ctcgcagaac
    17821 ctggagtggc tgcggcccta cgagctcgcc gaactgaacc cggccgacgc cgaacggctg
    17881 ggcgtcgtcg aggacgagct ggtgcgcgtg agctcacgcc gcggttcgat caccacgcgg
    17941 gtcacgctca ccgagcgcgt gcccccgggg acgatgttca tgacgttcca cttcaaggag
    18001 tccccggtca acgagctcac caacgccgcc ggcgacccgg tgacgatgac ggcggagttc
    18061 aaggtgtgcg ccgtcaggat ggacaaggag cccgccgcgg ccgagtcgca cggggccgcg
    18121 gcgcggtaag aagatcggcg ctgcgacgcg gcgcgatacg agacacccga gaggccgggg
    18181 cgcgccgcgc cccggcctct cgcacgtacg tcgcgcccgt gtccgagcgg tcaggccggg
    18241 cggcgcagcc ggccccggcc gtggacgatc gccgccagcg cgagggggac ggtgaggacg
    18301 gcgcttgtca gcgtcgccgc cgaggcgagg tcgctctcca gcgcgtgcag gccgaacgcg
    18361 gccatggaga gcaggccgct ggcgacgagg gtccagctca tccagtgcag cgcgccgggc
    18421 ctgcgcagca gcggccacgc gatgatgatc gccccaaggc cccagatggc gtacgccagc
    18481 aggtcgaacg tgtacggccc gccatgcacg ttgttgaagt accagggcgc ggccgccgcg
    18541 aggtcacgcc gcagcagcca gtcgaagaca gtgtactgcg acgcgtacgc ggccgccgag
    18601 agcggcgcat acgccgccac cagcgcgatg ccggcccaac tgcagagccg cgcctccgtg
    18661 atcccctctc cctccgccgg ggcgacgacg ccgagtgcga ccacggccat gaaggggacc
    18721 atggcggccg ggagcagcga ggcgaagaag aagccccagc ggtagacggt gtcgtcgcgc
    18781 gagaggtccg ccacctgcgc gacgacggtg tctccctgcg gcgcgccgag cgcggtcacc
    18841 gcagtcatca cgaggacgac aaagaggtat gccgcggcgg tggcgatgag acagcgcccg
    18901 agggctcgtc cggaggtcgc ggcgtctggc gaggcggtcg tcatgggagc tccttggagt
    18961 ccggctgcga gccggcgaag cgtcgccggc cggtggtccg tcggacgcta ccacaggcgg
    19021 ggtacgcggg ggcggcgggc accgaggcca cagcgcgcac cgccgcccct accccgccga
    19081 ccggcctacc ccccgcggga gaccgcctct ccgacggggt agccgccggc tccccccgcg
    19141 gtggccctcg aggcctttcc atatccctcc cccacgctca tgcattcacc accgtcgggc
    19201 gattcgccgc tcacggcacg gccgtagcct ctctccaggc aagtcaccca ggaggagaga
    19261 tcatgcggac caccaccatt tcggagcgcg aaggacggga ccaggcgagt gagacgctgg
    19321 cccgcctcga gcgcgagttc accggcgagt ccctgttcac cgagcggggt cccggccgcg
    19381 ccgtcgtgac ggcgcgcggc ctcacccgcg tctacggcaa gggcgacgcc caggtgcgcg
    19441 ccctggccgg cgtcgacatc gacatcaccg aaggccggct cacggcgatc atgggcccct
    19501 cgggatctgg caagagcacg ctcatgcaca tcctggccgg cctcgaccag cccaccggcg
    19561 gccgggtcag catcgacggc gtcgagatca cccggctcaa ggagaagaag ctcacgcagc
    19621 tgcgccgcga caagatcggc ttcatcttcc agagcttcaa cctgctcgcg accctgaccg
    19681 cggacgagaa catcgtcctg ccgctctcgc tcgcccgccg cagggcggac gag
//
LOCUS       DASUMX010000285         2688 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_682575, whole genome shotgun sequence.
ACCESSION   DASUMX010000285 DASUMX010000000
VERSION     DASUMX010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_682575"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1137)
                     /gene="gpmI"
                     /locus_tag="VFZ86_13595"
     CDS             complement(<1..1137)
                     /gene="gpmI"
                     /locus_tag="VFZ86_13595"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HEX5643351.1"
                     /translation="MTHPGAGGVTYRPVVLVVMDGWGVAPPGPDNAISIARTPVFDRL
                     VAEYPHGVLDASGPAVGLPPGQMGNSEVGHLNIGAGRVVYQDLTRISLAIEDGSFFEN
                     RVLRQACAKAAGRRSRLHLLGLVSGGGVHSDMGHLVACLDLAGREKVRDVVVHAFLDG
                     RDTPPRSAKGYLATIQEAMDRLGVGRFGVISGRYYAMDRDTRWDRVKLAYDALVHAQG
                     FFAAGAQAAVDAAYARGENDEFVRPTVVAPEHDARVRDGDVCIFFNFRPDRARELTRA
                     LTEPGFAEFDRGARPPSTDFVTMTQYKKEFPLPAAFPPDHPEHVLADVLAEHGLKQLH
                     IAETEKYAHVTFFFNGGAEAPKTGEERVLVPSPKVATYDLQPEMS"
     gene            complement(1134..>2688)
                     /gene="tpiA"
                     /locus_tag="VFZ86_13600"
     CDS             complement(1134..>2688)
                     /gene="tpiA"
                     /locus_tag="VFZ86_13600"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEX5643352.1"
                     /translation="SLGEVYVNDAFGTAHRAHASTEGVTHYLPSVAGLLMTKEIEVLG
                     RLLRDPSRPFVVVLGGLKVSDKISLIRHMLTIADTVLIGGAMANAFLAAKGYEVGASK
                     GAGDEVTVAGEILAEAGGTHGRLVIPTDVVVAREAAAGAPSRVALADGIAADEMALDV
                     GPQTTAEFERQLRGAGTIYWNGPMGLFEIAEFAAGTRAVGEAIVAAAAVTVAGGGDTV
                     SAVRAFGLEGRLTHVSTGGGASMEFLEGRALPGVEALMDRTAAATTAGRRPLMAGNWK
                     MYKTPAQTKEFFTAFRALVAGVDDRDVLICPPTVDLPTALACVVDTRIAIGAQTMHYA
                     AEGAYTGETAPGMLAELGVPYVILGHSERRQYYNENDADLAKKVRAALDAGLLPILCC
                     GETLEEREGGRTESKVGGQLDAGLAEVTPAELAKVAIAYEPIWAIGTGVTATPEQAQE
                     TVAFCRRKMRERFSDAADGVRILYGGSVKPDNIDVLMAQPDIDGVLVGGASLDPQGFS
                     RIVRFAEPV"
BASE COUNT          351 a          951 c          999 g          387 t
ORIGIN      
        1 gctcatctcc ggctgaagat cgtaggtcgc gaccttcggg cttggaacga gaacgcgctc
       61 ctcgcccgtt ttcggcgcct cggcaccccc gttgaagaag aacgtcacgt gcgcgtactt
      121 ctccgtctcg gcgatgtgca gctgcttcag gccgtgctcg gcgagcacgt ccgccagcac
      181 gtgctccggg tggtccggcg ggaacgccgc aggcagcggg aactccttct tgtactgcgt
      241 catcgtcacg aagtcggtgc tgggcgggcg ggcgccccgg tcgaactcgg cgaagccggg
      301 ttcggtgagg gcgcgcgtga gctcccgcgc cctgtccggg cggaagttga agaagatgca
      361 cacgtcgccg tcgcgcacgc gcgcgtcgtg ctccggcgcg acgaccgtcg ggcgcacgaa
      421 ctcgtcgttc tcgccgcggg cgtacgccgc gtccaccgcg gcctgcgcgc cggcggcgaa
      481 gaagccctga gcatgcacga gggcgtcgta ggcgagcttg acgcggtccc agcgcgtgtc
      541 gcggtccatg gcgtagtagc ggccgctgat gacgccgaag cggccgacgc cgagccggtc
      601 catggcctcc tggatcgtcg ccaggtagcc cttggcgctc cgtggcggcg tgtcgcggcc
      661 gtcgaggaac gcgtggacga ccacgtcacg gaccttctca cgccctgcca ggtcgaggca
      721 ggcgacgagg tgccccatgt cgctgtggac gccgcccccg gagacgagcc ccaggaggtg
      781 gaggcggctc cggcgccccg ccgccttggc acaggcctgc cggagcacgc ggttctcgaa
      841 gaagctgccg tcctcgatgg cgaggctgat ccgcgtgagg tcctggtaga cgacgcggcc
      901 ggcgccgatg ttgaggtggc cgacctcgga gttgcccatc tggcccggcg gcagccccac
      961 ggccgggccg gacgcgtcga ggacgccgtg cgggtactcc gccacgagcc ggtcgaagac
     1021 cggcgtgcgg gcgatgctga tggcgttgtc cggtcccggc ggggcgacgc cccagccgtc
     1081 catgacgacg aggacgaccg ggcggtacgt caccccaccg gcgccgggat gggtcacacg
     1141 ggctccgcga atcggacgat gcggctgaac ccctgcgggt cgaggctcgc cccgccgacc
     1201 agcacgccgt cgatgtccgg ctgcgccatg agcacgtcga tgttgtccgg cttgacgctg
     1261 ccgccgtaga ggatgcgcac gccgtcggcg gcgtcgctga agcgctcgcg catcttgcgg
     1321 cggcagaacg ccaccgtctc ctgcgcctgc tcgggcgtgg cggtgacgcc ggtgccgatg
     1381 gcccagatgg gctcgtaggc gatggctacc ttggccagct cggcgggcgt gacctcggcg
     1441 aggccggcgt cgagctggcc gcccaccttg ctctccgtcc ggccgccctc ccgctcctcg
     1501 agcgtctcgc cacagcagag gatcggcagc aggccggcgt cgagcgccgc ccggaccttc
     1561 ttcgcgaggt cggcgtcgtt ctcgttgtag tactgccgac gctccgagtg gccgaggatg
     1621 acgtagggca cgccgagctc ggcgagcatg cccggcgccg tctcgccggt gtaggcgccc
     1681 tccgcggcgt agtgcatggt ctgggcgccg atggcgatgc gcgtgtcgac cacgcaggcg
     1741 agggccgtcg gcagatcgac ggtcggcggg cagatgagga cgtcgcggtc gtcgacgccg
     1801 gcgacgagag cacggaaggc ggtgaagaac tccttcgtct gcgccggcgt cttgtacatc
     1861 ttccagttgc ccgccatgag cgggcggcgt cccgccgtcg tcgcggccgc ggtgcggtcc
     1921 atgagcgcct ccacgccggg cagcgcccgg ccctccagga attccatgga ggcgccgccg
     1981 ccggtggaca cgtgggtgag gcggccctcc aggccgaacg cgcggacggc cgacacggtg
     2041 tcgccgccgc cggccaccgt cacggcggcg gcggccacga tggcctcgcc gacggcgcgc
     2101 gtgccggcgg cgaactccgc gatctcgaac aggcccatgg ggccgttcca gtagatggtc
     2161 ccggcgccgc gcagctggcg ttcgaactcc gccgtggtct gcgggccgac gtcgagcgcc
     2221 atctcgtcgg cggcgatgcc gtcggcgagc gcgacgcgcg agggcgcgcc ggcggccgcc
     2281 tcgcgcgcca cgaccacgtc ggtggggatc acgagccggc cgtgcgtgcc tccggcctcg
     2341 gccaggatct cccccgccac cgtgacctcg tccccggcgc ccttggaggc gccgacctcg
     2401 tagcccttgg cggcgaggaa cgcgttggcc atggcgccgc cgatcagcac cgtgtcggcg
     2461 atggtgagca tgtggcggat gaggctgatc ttgtcggata ccttgaggcc gcccaggacc
     2521 accacgaacg gccgcgacgg gtcgcggagc aggcgcccga ggacctcgat ctccttggtc
     2581 atgagcaggc cggcgaccga cggcaggtag tgcgtgacgc cctcggtgga ggcgtgcgcc
     2641 cggtgcgccg tgccgaacgc gtcgttgacg tagacctcac cgagagac
//
LOCUS       DASUMX010000286         5609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1545278, whole genome shotgun sequence.
ACCESSION   DASUMX010000286 DASUMX010000000
VERSION     DASUMX010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 5609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5609
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1545278"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..121)
                     /locus_tag="VFZ86_13605"
     CDS             complement(<1..121)
                     /locus_tag="VFZ86_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013412389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HEX5643353.1"
                     /translation="MDTSKKTIILFSGDFDRVMAALIIANGAAAMGDDVTIFAT"
     gene            complement(121..396)
                     /locus_tag="VFZ86_13610"
     CDS             complement(121..396)
                     /locus_tag="VFZ86_13610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006520998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX5643354.1"
                     /translation="MRSTPGNEQLEPRLRPVREVIRDEHSMKAPILKALGDGPLTIPQ
                     IAAAVGRPTREVTFWVMGLRKYGWIAEIKEVDDEGYFPYALVPREES"
     gene            complement(393..2135)
                     /locus_tag="VFZ86_13615"
     CDS             complement(393..2135)
                     /locus_tag="VFZ86_13615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HEX5643355.1"
                     /translation="MSAGKIGVYVCQCGGNIGDHVDVDEVVAAIEKEQGVSVAKTAMF
                     TCSDATQSEIIEDAREQGLDGLVIASCSPKLHTFTFRDVAKRAGMNPFQYTQVNVREQ
                     CSWTHTDDHEGATEKAVRLVRAGINRTRLTDPLEPIVVETTPKSLVVGGGIAGLRAAL
                     GLAEIGLHVFLVEREAKLGGWVAGFREMFPHGRNGRELIGKLEEKVRSHPEITVFTSA
                     ELVAKSGSFGNYQATVAVSNGDRLEQIQVDVGSIVIATGFDTYQPEEGELGYGIDGVI
                     TIADFKKLLDETDGPLTYNGKPVRTIAYIYCVGSRQDESVEGANTYCSRYCCTAAVHA
                     ATQVAARPQRVHQYHFYRDMRTYGKYELLYAEALEQGSAFVKYPDDEAPAVEKDAKSG
                     GVTVTSKDLLSAGDEVALTADLVVLVTGMVARENEGLTKTLKLPVGQSGFYNEIHPKL
                     RPVETVVDGAYICGACQSPRTSSEAVASGLAAVTQSAAILKRGYAELDPLVATVDTDA
                     CTWCGACEFTCPYDAIAKVEEGGKELAAISRTACKGCGGCVPVCPSDAIDLQGYTDAQ
                     IKAMIDGMLEVVCQ"
     gene            complement(2132..4918)
                     /locus_tag="VFZ86_13620"
     CDS             complement(2132..4918)
                     /locus_tag="VFZ86_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006520996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5643356.1"
                     /translation="MSDPRKYDALVVGSGIGGMESALKLGDMGYKVLVVEKEPSVGGK
                     MILLSKVFPTLDCASCISTPKMGATIHHPNIDVMTYAEVEAIRGNGGNGNGNGPARYH
                     ARIKQKAKFVDEAACTGCRQCEMACNVAVPDEYNADMVSRRAAFIAFPQAVPKKAVIS
                     REGVSPCTYECPAGIKAHGYVARVRSGEYDQAFDLVLETTPLVGSLGRACYAPCEGEC
                     TRGELEGPLPIRRLKRFIADERYGQAEPKAIEKPEQNGKKVAVVGSGPAGLTAAWQLA
                     RKGYGVKIYEAAERAGGFLALGIPAYRLPNDVVDDDISNVTAIGVEIETGVRVEDPAK
                     LKDDGFDAVLVATGTHKAVQLRVPGEEKDGVMSALTFLADVKLGKNVDLDGRKVLVVG
                     GGNVAIDAARTARRLGAAEVHQASLECCEDMPAHDFEVEEAKAEGITLHDGWGVNHFT
                     GDGKVEGAVLKVCTCVFDPSGKFAPEYDEAQQQTVACDVVIVAAGMGADTEAFGLETN
                     PSRTLKAESGTLQTSLPHVFAAGDVVTGPSMITTAVGQGRRAAFMIDRFLQGEALDPD
                     AFDEALPVADKKSVLARQDVYDRREPFESHAAMSAAPTDFSETEAPMTEAEAFLGAAR
                     CLDCGVCSECHACVDVCPADCIDFSMREEEIEVEVDTVVLATGFKLFPADAKPEYGYG
                     RYANVITGMQMDRLLAPTRPFNAVVRPGDGKVPDRIAFILCTGSRDAQVGNPLCSRIC
                     CMYSIKHNQLIMGALPLADVTVHYMDVRAVGKGYDEFYKQAVDMGSMFIKGRVAKITE
                     KDNGDLIVRYEDIEGDGGIVEAEYDMVVLAVGVQPNTQVAGLFEDGALGLDEYFYVGE
                     ADDDMDPGRTNLPGVFVAGSASGVKDIPDSILHAGAAVAQAAAHIEKARGVEAAVNVP
                     EAAVNVPVKAEVTA"
     gene            complement(4915..5352)
                     /locus_tag="VFZ86_13625"
     CDS             complement(4915..5352)
                     /locus_tag="VFZ86_13625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006520995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HEX5643357.1"
                     /translation="MADVEASEGQAYDPRILVFSTNNISDPGIDLAGSSHMHYSPKVM
                     VINLPCTSAVKPSWIVYAMEQGFDGVFIAADGEECAYLPDCAERTSGIMQTAQDMLVE
                     KGLEPQRVRMAAVCSVCAEPFTNYMHEYAAALSGLGPARRAAS"
     gene            complement(5355..>5609)
                     /locus_tag="VFZ86_13630"
     CDS             complement(5355..>5609)
                     /locus_tag="VFZ86_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020489419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEX5643358.1"
                     /translation="TMSEDLTTIQAEKIVDARGTSCPGPILAAKKGIPGVSVGGVMEV
                     QATDSGTTKDLPAWCKKMGHDYLGVVEEPGYMRLFVKKMK"
BASE COUNT          771 a         1884 c         1962 g          992 t
ORIGIN      
        1 aggtggcgaa gatggtgacg tcgtcgccca tggcggcggc gccgttggcg atgatcagcg
       61 ccgccatgac gcggtcgaag tcaccgctga acaggatgat cgtcttcttg ctggtgtcca
      121 tcaggactcc tctcgcggga ccagggcgta cgggaagtag ccctcgtcgt cgacttcctt
      181 gatctctgcg atccagccgt acttgcgcag gcccatgacc cagaaggtga cctcgcgcgt
      241 gggtcttccg acggccgcgg cgatctgagg gatcgtcagc gggccgtcac cgagcgcctt
      301 gaggatcggg gccttcatgg agtgctcgtc ccggatcacc tcgcgcacgg ggcgcaggcg
      361 cggctcgagc tgctcgttgc ccggagtgct cctcattggc acaccacctc cagcatgccg
      421 tcgatcatcg ccttgatctg agcgtcggtg taaccctgca ggtcgatggc gtcggacggg
      481 cagacgggga cgcagccgcc gcagcccttg caggccgtcc tggagatcgc ggcgagctcc
      541 ttgccgccct cctcgacctt ggcgatggcg tcgtagggac aggtgaactc gcaggcgccg
      601 caccaggtgc aggcgtcggt gtccacggtg gcgaccagcg ggtcgagctc ggcgtacccg
      661 cgcttgagga tcgccgcgct ttgagtgacg gcggcgagcc ccgaggccac cgcctcggac
      721 gaggtcctgg ggctctggca ggcgccgcag atgtaggcgc cgtccaccac cgtctcgacc
      781 ggccgcagct tggggtggat ctcgttgtag aagccgctct ggcccacggg gagcttgagc
      841 gttttggtga gaccctcgtt ctcccgggcc accatgcccg tgaccaggac gacgaggtcg
      901 gcggtgaggg cgacctcgtc gcccgcggac agcaggtcct tggacgtcac cgtgacgccg
      961 ccggacttcg catccttctc cacggccggc gcctcatcgt ccgggtactt gacgaacgcc
     1021 gagccctgct ccagcgcctc ggcgtagagc agctcgtact tcccgtaggt gcgcatgtcg
     1081 cggtagaagt ggtactggtg cacgcgctgc gggcgcgccg cgacctgggt cgcggcgtgc
     1141 acggcggccg tgcagcagta gcgcgagcag taggtgttgg cgccctcgac gctttcgtcc
     1201 tggcggctgc ccacgcagta gatgtaggcg atcgtcctga ccggtttgcc gttgtaggtc
     1261 agcggtccgt cggtctcgtc gaggagcttc ttgaagtcgg cgatggtgat gacgccgtcg
     1321 atgccgtagc cgagctcgcc ctcttcgggc tggtaggtgt cgaaaccggt cgcgatcacg
     1381 atcgagccga cgtccacctg gatctgctcc aggcgatcgc cattgctgac ggcgaccgta
     1441 gcctggtaat tgccgaagct gccgctcttg gcgacgagct ccgcgctggt gaagaccgtg
     1501 atctccgggt gggagcggac cttctcctcg agcttcccga tgagctcccg gccgttgcgg
     1561 ccgtggggga acatctcgcg gaagccggcc acccagccgc cgagcttggc ctccctctcc
     1621 acgaggaaca cgtggaggcc gatctcggcg aggccgaggg cggcgcgcag tcccgcgatc
     1681 ccgccgccga ccacgaggct cttgggcgtc gtctcgacga cgatcggctc cagcgggtcg
     1741 gtgaggcgcg tccggttgat gccggcgcgg acgagccgca ccgccttctc ggtcgcgccc
     1801 tcgtggtcgt cggtgtgcgt ccacgagcac tgctcgcgca cgttcacctg cgtgtactgg
     1861 aaggggttca tgcccgcgcg cttggcgacg tcgcggaacg tgaacgtgtg cagcttgggc
     1921 gagcaggagg cgatgacgag cccgtcgagg ccctgctccc gcgcgtcctc gatgatctcg
     1981 ctctgcgtcg cgtcagagca ggtgaacatc gccgtcttgg cgacactgac gccctgctcc
     2041 ttctcgatgg cggcgacgac ctcgtcgacg tcgacgtgat cgccgatgtt gccgccgcac
     2101 tggcagacat agacgcctat cttgccggcg ctcatgccgt cacctccgcc ttgaccggca
     2161 cgttgacggc cgcttccggc acgttgaccg ccgcttccac gccccgcgcc ttctcgatgt
     2221 gtgcggcggc ctgggcgacc gcggcgccgg cgtggaggat ggagtccggg atgtccttga
     2281 cgcccgaggc cgagcccgcg acgaacacgc ccggcagatt ggtgcggccg gggtccatgt
     2341 cgtcgtcggc ctcgccgacg tagaagtact cgtcgaggcc cagggcgccg tcctcgaaga
     2401 gcccggcgac ctgcgtgttg ggctgcacgc cgacggcgag gacgaccatg tcgtactcgg
     2461 cctcgacgat gccgccgtcg ccctcgatgt cctcgtagcg gacgatgagg tcgccgttgt
     2521 ccttctcggt gatcttggcg acgcggccct tgatgaacat ggagcccatg tccacggcct
     2581 gcttgtagaa ctcgtcgtag cccttgccca cggcgcgcac gtccatgtag tggaccgtga
     2641 cgtcggcgag cggcagggcg cccatgatca gctggttgtg cttgatggag tacatgcagc
     2701 agatgcgcga gcagaggggg ttgcccacct gcgcgtcgcg cgagcccgtg cacaggatga
     2761 aggcgatgcg gtccggcacc ttgccgtcgc cggggcgcac caccgcgttg aacgggcgcg
     2821 tcggtgcgag caggcggtcc atctgcatgc cggtgatgac gttggcgtac ctgccgtagc
     2881 cgtactcagg cttggcgtcg gcagggaaga gcttgaagcc ggtcgcgaga acgaccgtgt
     2941 ccacctcgac ctcgatctcc tcctcgcgca tgctgaagtc gatgcagtcg gcagggcaga
     3001 cgtcgacgca ggcgtggcac tcggagcaca cgccgcagtc cagacagcgg gcggcgccga
     3061 ggaaggcttc tgcttcggtc atcggcgcct cggtctcgct gaagtcggtc ggcgccgcgc
     3121 tcatcgcggc gtgcgactcg aacggctcgc gccggtcgta gacgtcctgc cgcgcgagca
     3181 cgctcttctt gtcggcgacc gggagcgctt cgtcgaacgc gtccggatcc agggcctcgc
     3241 cctgcaggaa gcggtcgatc atgaaggccg cgcgccggcc ctggccgacg gcggtggtga
     3301 tcatgctggg accggtgacg acgtcgccgg cggcgaagac gtgcggcagc gacgtctgca
     3361 gcgtgccgga ctccgccttg agcgtccggc tggggttggt ctcgaggccg aacgcctcgg
     3421 tgtccgcgcc catgccggcg gcgacgatga cgacgtcgca cgccaccgtc tgctgctggg
     3481 cctcgtcgta ctccggcgcg aacttgccgg aggggtcgaa gacgcaggtg cagaccttga
     3541 gcacggcgcc ttcgaccttg ccgtcaccgg tgaagtggtt gacgccccag ccgtcgtgca
     3601 gcgtgatgcc ctccgccttg gcctcctcga cctcgaagtc gtgggccggc atgtcctcgc
     3661 agcactcgag gctggcctgg tggacctccg cggcgccgag gcggcgcgcc gtgcgcgccg
     3721 cgtcgatggc cacgttgccg ccgccgacca cgaggacctt gcggccgtcg aggtcgacgt
     3781 tcttgccgag cttgacgtcg gcgaggaagg tgagggcgct catgacgccg tctttctcct
     3841 cgccgggcac gcgcaactgg accgccttat gcgtgcccgt cgccacgagg acggcgtcga
     3901 acccgtcgtc cttgagcttc gcgggatcct cgacccggac gccggtctcg atctcgacgc
     3961 cgatggcggt gacgttgctg atgtcgtcgt cgacgacgtc gttggggagg cgataggcgg
     4021 ggatgccgag cgccaggaag ccgccggcgc gctcggccgc ctcgtagatc ttcacgccgt
     4081 agcccttgcg cgcgagctgc caggcggcgg tgaggccggc cgggccggag cccacgacgg
     4141 cgaccttctt gccgttctgc tccggcttct cgatcgcctt cggctccgcc tggccgtacc
     4201 gctcgtcggc gatgaagcgc ttgaggcggc ggatcggcag cgggccctcg agctcgccgc
     4261 gtgtgcactc gccctcgcac ggcgcgtaac aggcgcgacc gagcgagccg acgagcgggg
     4321 tcgtctcgag gaccaggtcg aacgcctggt cgtactcacc gctgcgcacg cgcgcgacgt
     4381 agccgtgcgc cttgatgccc gcggggcact cgtaggtgca cggcgagacg ccctcgcggc
     4441 tgatgaccgc cttcttcggc accgcctgcg ggaaggcgat gaacgccgcc cgccgcgaca
     4501 ccatgtcggc gttgtactcg tccggcacgg cgacgttgca ggccatctcg cactgacgac
     4561 agccggtgca ggcggcctcg tcgacgaact tggccttctg cttgatcctg gcgtggtagc
     4621 gggcggggcc gttgccgtta ccgttgccgc cgttgccgcg aatggcctcc acctcggcgt
     4681 aggtcatcac gtcgatgttg gggtggtgga tggtggcgcc catcttgggc gtcgagatgc
     4741 agctggcgca gtcgagcgta gggaagacct tgctgagcag gatcatcttg ccgccgacgc
     4801 tcggctcctt ctcgacgacc aggaccttgt agcccatgtc gccgagcttg agcgccgact
     4861 ccatgccgcc gatgccgctg ccgacgacga gcgcgtcgta ctttctggga tcactcatga
     4921 ggccgccctt ctggcagggc cgaggcccga caaggcggcc gcgtactcgt gcatgtagtt
     4981 cgtgaagggc tccgcgcaga cggagcagac cgcggccatg cgtacgcgct ggggctccag
     5041 gcccttctcg accagcatgt cctgtgcggt ctgcatgatg ccggaggtgc gctccgcgca
     5101 gtccggcagg tacgcgcact cctccccgtc cgcggcgatg aagacgccgt cgaagccctg
     5161 ctccatcgcg tacacgatcc agctcggctt gaccgcgctg gtgcacggca ggttgatgac
     5221 catgaccttg ggcgagtagt gcatgtgcga gctgcccgcc aggtcgatgc ccgggtcgga
     5281 gatgttgttc gtgctgaaga ccaggatgcg cgggtcgtac gcctggccct cggatgcctc
     5341 tacgtccgcc atcgtcactt catcttcttg acgaagaggc gcatgtagcc gggctcctcg
     5401 acgacgccga ggtagtcgtg acccatcttc ttgcaccacg cggggaggtc cttggtggtg
     5461 ccgctgtcgg tcgcctggac ttccatgacc ccgccgaccg aaacgccggg gatgcccttc
     5521 ttggcggcga ggatcgggcc ggggcagctg gtgccgcgcg cgtcgacgat cttctcggcc
     5581 tggatggtgg tgaggtcctc tgacatggt
//
LOCUS       DASUMX010000287         3482 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1833637, whole genome shotgun sequence.
ACCESSION   DASUMX010000287 DASUMX010000000
VERSION     DASUMX010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3482
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1833637"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="VFZ86_13635"
     CDS             <1..197
                     /locus_tag="VFZ86_13635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HEX5643359.1"
                     /translation="DARAGKGETYGGIDYYGHTKKEFEQRAKRLGITGYSRMRKAELV
                     KKIEQRERSESARQRGRKAA"
     gene            complement(417..2222)
                     /locus_tag="VFZ86_13640"
     CDS             complement(417..2222)
                     /locus_tag="VFZ86_13640"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEX5643360.1"
                     /translation="MRVSQLLLPTVKEDPAGAEAVSHKLMVRAGLVRQLAAGIYVYLP
                     AGWRVMQKIEQIIREEMDAIGCQEMLMPVMQPAEIWQESGRWDAIGGEMFRLKDRKGG
                     DMALAMTHEEVVTWLAAREVHSYKQLPQMWYHFQTKERDEARPKSGILRTREFIMKDS
                     YSLDVSEEALQESYRLHIGAYDRIYRRCGLEFMMVEGDSGMMGGAISHEYMAYADAGE
                     DEIVFCRECGYASNVETAVAGADPEPPASELAPRGAADAPFARATGAPVELTAEAELD
                     TPDCKTIDQIAAYLGLPARAFIKALVVVPEGGGEAGTGGAAAASGPTPAGPVMVLVRG
                     DHELGELKLRKALGADFRMATADEVLAAQGVEPGFVGPVPTPLPVFADHALRRGHYVA
                     GANKAGFHRSGVTLAQVPQATFTDLRDARPGDPCAQCSGDLEGARVIEVGNIFQLGLK
                     YSVPMGATFLDEDGKERPIVMGSYGIGLARIAAAAVEQHHDDHGICWPASIAPFRVHL
                     ILVRASDDVQRSLADDLYTQMGQAGADVVYDDRDLSPGIKFKDADLLGCPAQIVVGKR
                     AGEGFVELKKRDGGERRDVPVADLAATLEELLAGA"
     gene            2329..>3482
                     /locus_tag="VFZ86_13645"
     CDS             2329..>3482
                     /locus_tag="VFZ86_13645"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX5643361.1"
                     /translation="MRILFVLPELPHPPFTGAHTRPLTLLRAAARSHEVAVVGAAPKD
                     ADLSLVRELCADVRVAPAGAAAPARRRGMTAGRRLVTPVPLVSAGRSRSLAGLVDEMV
                     EEWQPEAMLAETLYAAHYRVPDLPTIVDLPEVPSGLCESAAAARPLRYLAANLQAATS
                     RRYERHLLEGTLTMTINDGDRDRLATLGVEAYAVPLAIALPPEDAIYERSSGARHDVA
                     VRLLFIGSFKHGSNRDAARWLVRRLAPELRTERVPFAMVIAGLHAPSWLRKAGGAGVT
                     VISDAPDLDPLYRSADIVLAPLPHGGGTKTKTLEAMAWGLPVIGTPQAFTGIPQLDGE
                     AFVVDPLDPLRVAMRIAALRDDPELRLHLGWAARDYVAAAHAPELAETRAA"
BASE COUNT          452 a         1286 c         1264 g          480 t
ORIGIN      
        1 aggacgcccg cgccggcaag ggcgagacct acggcggcat cgactactac ggtcacacca
       61 agaaggagtt cgagcagcgc gccaagcggc tcggcatcac cggctacagt cgcatgcgca
      121 aggcggaact ggtgaagaag atcgagcagc gcgaacgctc cgagtcggcc aggcagcggg
      181 gtcgcaaggc ggcgtgagcc gaagctgcgg cgcgaaccgc gacctgggcg tgagccgcgc
      241 ccggcttggc cctggtctca gcagccgcgg ggcgggaggt agtgcgaaac gctctggcac
      301 caccggacgt acgccccaag gcacaaccgt acacctgcgc gtacgtccgg acgtgcgagt
      361 ccgcgcagcg ccatgccacc ccccggcggc cgcgagcgag tccgcgcccg gcggcctcag
      421 gccccggcga gcagctcctc cagggtggcg gcgaggtccg cgacgggtac gtcgcgccgc
      481 tcgccgccgt cacgcttctt gagctccacg aagccctcgc cggcgcgctt gcccaccacg
      541 atctgcgccg ggcagccgag gaggtccgcg tccttgaact tgatgccggg gctcaggtcg
      601 cggtcgtcgt acacgacgtc ggcgccggcc tggcccatct gtgtgtacag gtcgtcggcc
      661 aggctgcgct gcacgtcgtc ggacgcgcgc acgaggatga ggtgcacgcg gaacggggcg
      721 atgctggccg gccagcagat gccgtggtcg tcgtggtgct gctcgacggc cgcggcggcg
      781 atgcgcgcga ggccgatgcc gtagctgccc atgacgatgg gacgctcctt gccgtcctcg
      841 tccaggaacg tcgcccccat gggcaccgag tacttgagcc cgagctggaa gatgttgccg
      901 acctcgatga cgcgcgcgcc ctcgaggtcg cccgagcact gcgcgcacgg gtcgccgggg
      961 cgcgcgtcgc gcaggtcggt gaacgtggcc tgcggcacct gcgccagcgt gacgccgctt
     1021 cggtggaagc cggccttgtt ggcgccggcg acgtagtggc cccggcgcag ggcgtggtcg
     1081 gcgaagaccg gcagcggcgt cggcacgggg ccgacgaagc cgggctcgac gccctgggcc
     1141 gcgagcacct cgtcggcggt ggccatgcgg aagtcggcgc cgagcgcctt gcgcagcttc
     1201 agctcgccga gctcgtggtc gccgcggacg aggaccatga cggggccggc gggcgtaggg
     1261 ccggaggcgg cggctgcgcc gcccgtgccg gcctctcccc cgccctcggg caccaccacg
     1321 agcgccttga tgaaggcgcg cgccggcagg ccgaggtagg ccgcgatctg gtcgatcgtc
     1381 ttgcagtccg gtgtgtccag ctcggcctcg gcggtcaatt ccaccggcgc gccggtcgca
     1441 cgggcgaacg gcgcgtccgc ggcgccgcgc ggcgcgagct cgctcgccgg cggctcgggg
     1501 tcggcgccgg cgacggcggt ctcgacgttg gaggcgtagc cgcactcgcg gcagaagacg
     1561 atctcgtcct cgccggcgtc ggcgtaggcc atgtactcgt ggctgatcgc gccgcccatc
     1621 atgcccgagt cgccctcgac catcatgaac tcgaggccgc agcgccggta gatgcggtcg
     1681 taggcgccga tgtgcaggcg gtagctctcc tgcagggcct cctcgctcac gtcgaggctg
     1741 tacgagtcct tcatgatgaa ctcgcgcgtg cgcaggatgc cgctcttggg ccgcgcctcg
     1801 tcgcgctcct tggtctggaa gtggtaccac atctgcggga gctgcttgta gctgtggacc
     1861 tcccgcgccg ccagccaggt gacgacctct tcgtgcgtca tggcgagggc catgtcgccg
     1921 cccttgcggt ccttgagccg gaacatctcg ccgccgatcg cgtcccagcg cccgctctcc
     1981 tgccagatct ccgccggctg catgaccggc atgagcatct cctggcagcc gatggcgtcc
     2041 atctcctcgc ggatgatctg ctcgatcttc tgcatgacgc gccagccggc cggcaggtag
     2101 acgtagatgc ccgcggcgag ctggcgcacg aggccggcgc ggaccatgag cttgtggctc
     2161 acggcctccg cgccggccgg atcctctttg acggtgggca gaagcagctg gctgacgcgc
     2221 atggggcctc gcgatcgtgg ggcttgcgga cgaggcccta tgctaccaaa cgcggccggc
     2281 ccggcggtcg aacggcgggc ggagcgcacg agtggtacca tccgcccgat gcgcatcttg
     2341 ttcgtcctgc ccgagctgcc ccacccgccg ttcaccggcg cgcacacgcg gccgctgacg
     2401 ctgctgcgtg cggccgcgcg ctcgcacgag gtcgcggtgg tcggcgcggc gcccaaggac
     2461 gccgacctga gcctcgtccg cgagctgtgc gcggacgtcc gggtcgcgcc ggcgggcgca
     2521 gccgccccgg cgcgccggcg cggcatgacc gccggcaggc ggctggtcac gccggtccct
     2581 ctggtcagcg ccgggcgctc gcgcagcctc gccggcctcg tggacgagat ggtcgaggag
     2641 tggcagcccg aggcgatgct ggccgagacc ctgtacgcgg cgcactaccg cgtccccgac
     2701 ctcccgacga tcgtcgacct gcccgaggtc cccagcggcc tgtgcgagtc ggccgcggcg
     2761 gcgcgaccgc tgcgctacct cgccgccaac ctccaggcgg cgaccagccg gcggtacgag
     2821 cgtcacctgc tcgagggcac cctgacgatg acgatcaacg acggcgaccg cgaccgtctc
     2881 gccaccttgg gcgtcgaggc ctacgccgtg ccgctggcga tcgcgctgcc gcccgaggac
     2941 gcgatctacg agcgcagctc gggcgcgcgc cacgacgtcg ccgtgcgcct gctgttcatc
     3001 ggctcgttca agcacgggtc caaccgtgac gcggcgcgct ggctggtgcg ccgcctggcc
     3061 cccgagctgc gcacggagcg cgtgcccttc gcgatggtca tcgcggggct gcacgcgccc
     3121 tcgtggctgc gcaaggccgg cggcgccggc gtcaccgtca tctccgacgc ccccgacctc
     3181 gaccccctgt accgcagcgc cgacatcgtc ctcgcgccgc tcccgcacgg cggcggcacc
     3241 aagaccaaga ccctggaggc gatggcgtgg ggactccccg tcatcggcac gccgcaggcg
     3301 ttcaccggga tcccccagct ggacggtgag gcgttcgtcg tcgacccgct cgacccgctg
     3361 cgtgtggcca tgcgcatcgc cgcgctgcgc gacgacccgg agctgcggct gcacctcggc
     3421 tgggccgcgc gggactacgt cgccgcggcc cacgcgccgg agcttgccga gacgcgcgcg
     3481 gc
//
LOCUS       DASUMX010000288        16576 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1401969, whole genome shotgun sequence.
ACCESSION   DASUMX010000288 DASUMX010000000
VERSION     DASUMX010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 16576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16576
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1401969"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            351..2084
                     /locus_tag="VFZ86_13650"
     CDS             351..2084
                     /locus_tag="VFZ86_13650"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEX5643362.1"
                     /translation="MSLLKGRRYFDRRSVGRDAAGGLTLGIESIPDAMASGVLAGVNP
                     IYALYAVMLATPVGALFSSSVFMSVQTTSAMSLLVADVPQVHGGADAAGALFMLTLMT
                     GVIMLAMGLLKLGSYLRFVPNAVLTGFINGVAVLIILGQLGELTGTSPEGGNKVEQAG
                     DLLRNWQDIDPRAFAVGLTAIVLIVALQNTRLKSWGMVVALVMASLLVPLFNWGDVAL
                     VTDVASIPDSLPLPTLPDFSALPALIVPAIALAFVGLVQGSGISQSFTNPDGRYPSAS
                     GDFVGQGAANVAASLFQGMPVGGSLSATALVHNAGVRSRAANIIAGVTIAVVLLLFSG
                     LVGKLAMPALAGLLIVVGFQTLKPHQIDLVVRTGWLQAVVVVTTFVLTLLVPLQYAVL
                     IGVAVSILLHWVKQSNQMVLKEWVFPPGQALPREQDPPDRLPGDHVTVITPYGRMFFA
                     TARLMEEQLPEVTTDSRRAVLILNLREHDELGSTFLGMFDRYAESLREHGAKLMLAEV
                     GPHLYGQLHNTGRLRALKPRNVLRRSDTVDASIVEAIDTADEWIEAAAREVAEAGAEN
                     GTGPEHPAETT"
     gene            2221..2595
                     /locus_tag="VFZ86_13655"
     CDS             2221..2595
                     /locus_tag="VFZ86_13655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643363.1"
                     /translation="MNSKWVGWAATGGILMVIVGGFKAISGLIGLFNDEWLVRGFDAY
                     YFVDISALAWWYLLVGLFLLFAGLAVLNGRAWGRWVGVFAAGVAIISELLWLPIYPVW
                     SILLITMYVFMMIGLIAVGSED"
     gene            2731..4236
                     /locus_tag="VFZ86_13660"
     CDS             2731..4236
                     /locus_tag="VFZ86_13660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018772616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 domain-containing protein"
                     /protein_id="HEX5643364.1"
                     /translation="MAEPKKIAHPSVEERTAHGKAARKAVSPSSHAGWEPAADRPDPV
                     ALLQEQNARREPDLVPVRHGRMMVSPFTFYRGAARIMAADLKETPRAGLTVQLCGDAH
                     LSNFGIFASPERQLVFDLNDFDETLPGPFEYDVARLAASFTIAARNNGFGKADEQSAT
                     RAVVRAYREAMAGFARMRVMDLWYARITEEDLMAALRSMAPEPAPFTKKATARAGKQG
                     KKAKRQAREADARRATEKAGELRIQKAADKARTRDSLQALSKLAEYVDGGYRIVSQPP
                     IVVPARDLLETYGISPDEVHHVIHQEYRAYRATLQSDRRHLLEKFELVDVARKVVGVG
                     SVGTRAFIVLLLGRDEQDPLFLQVKEATYSVLEDHLPKSRHQPGSRVVHGQRMMQAAS
                     DIFLGWTKGVQEGRFLYWRQLRDMKGSADIESLQPFGLEYYARECGWTLARAHARSGD
                     PVAISAYLGKSDKFDTAMTDFSARYADQNERDYEAFLEAIRSGRLEAIEGL"
     gene            4385..5608
                     /locus_tag="VFZ86_13665"
     CDS             4385..5608
                     /locus_tag="VFZ86_13665"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX5643365.1"
                     /translation="MTSEQGAPTTAPAATGQDAHKAAAAGATSRWAVAAWASDIGKWS
                     GIIILVILLVVVVFFLLAATRVLLLAALFAVLFAGTFLPMVDWLQKHHVKRGLGAVIV
                     SALLILLGIAVGALIVYSVIKQLPTVEARLDDAWTEIQKALTSTSVSQEQIDSLKSNV
                     QNLAKDAASGLAGTVVDLFGGVASLIFGAFISLNILVWSLIQGRKLARWASKRMGPVP
                     PAVGYEIFANSARFFRGYLFGSTLVGLFNGAVMGIGALIIGVPLAGTIFVVGWFTNYI
                     PFFGAIVSGAFAVLIALGAGGPAMAIPMLIIVIVSNGYLQTIVSQFALGSALKLHPLA
                     VLFATTAGSMLFGAVGGVFAAPFLKIFLDARLKMKAAGLFSSSPTAGSAAAGAPPSDQ
                     GAGPPGTAESLPAEA"
     gene            complement(5630..7363)
                     /locus_tag="VFZ86_13670"
     CDS             complement(5630..7363)
                     /locus_tag="VFZ86_13670"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HEX5643366.1"
                     /translation="MRRSSLDKLREDLRLQQVYNTLLRYGYDTVLDRTAFLGGFRHAM
                     QSWVWRMPRGWEEPPAAVKLRLMLEELGPTYVKIGQIVSSQASVLPVDMATELSRLQD
                     AVPPFPSDQVREILIEELDGPPEELYASFAPEPFAAASTAQVHRATLHDGTLVAVKVQ
                     RPDIQIKIRADLGILLNAARVASTRSETVRSLDISGMLEQFSSGVLEELDYRGEAYNA
                     MRLAENMQGLPGIHVPRIYADLSTSRVITQEFVVGVKVSDVAAIEAAGLDRDLIARNA
                     LRALIKQLPVDGFFHADPHPGNLLLNLDTGVLTFIDLGMMGELDLQKRLRLGQLMIVA
                     QQGSVEGMADALRGMSTPFKGDVDEKAFRRDFARRMGRYMSRGRVASFGEVANEGFEL
                     LRIHGLRLDADLTLAVKALVQTESVARALSTDSGLLSEGVPLLRDMALEAVSGEKIVD
                     EAKKQLMGVAGQVVERIPTLSEATTKWLDQYQKGRFEVTVDTSQLSKEVTRLTRLGRE
                     IVIAVMLVGMLVGSAVASYGIATVELEGTIWNLLERIAPVGFVLSLILSFLIVLRLTW
                     RWLRGASADED"
     gene            complement(7360..7926)
                     /locus_tag="VFZ86_13675"
     CDS             complement(7360..7926)
                     /locus_tag="VFZ86_13675"
                     /inference="COORDINATES: protein motif:HMM:NF015953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEX5643367.1"
                     /translation="MAVTTPKRHVSLHFRVRPLLLRWLVSALCLALTVLIVPHVFMTG
                     EYRVLSWLIISAVFGLLMTFVKPLLQLLLLPLIFVSYGLVIVLINTIVVWILSWIFPE
                     RFQVEHILWALVAGLVSGLLIGLFENLFGLTPPIVQGGPDALREEMARAKPGHLEREL
                     LDVAVKGQEKMKEMTAKSDDEAPEEEPS"
     gene            complement(8059..9048)
                     /locus_tag="VFZ86_13680"
     CDS             complement(8059..9048)
                     /locus_tag="VFZ86_13680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643368.1"
                     /translation="MRHGGIRRGMWVVAGAVALAPAVLAAALALAGCGAPREVAAATY
                     GAAASPLYDRGYKCDLVERLPEPPELVIFGGSRAQRFEPTLAEELTGLPAFNFAVQNS
                     RPEDVYAMARHLFWRAPGVKLRCFWALQATTLSDAPFHPGLLAEPRLSRFLPEDLLAK
                     QRGISVSREGRELSPDNRFTARGSLVYNSYDARLERGIPFEQTLAGYLSALVPRAGAP
                     AAYDSARARRYFERTLQLFNLHDVEPVLVIMPYHPTALAAFRAVGWGAKEQALKTYLK
                     SLRGTYRFHLLDYTDIASFHGSRDAFYDGAHVKSVNARRILRQAVMDAPAAFR"
     gene            9153..10262
                     /gene="purM"
                     /locus_tag="VFZ86_13685"
     CDS             9153..10262
                     /gene="purM"
                     /locus_tag="VFZ86_13685"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006472437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEX5643369.1"
                     /translation="MADEHGFTYKKAGVDVEAGNRAVELMKRRLRSTQHPLVLGGLGG
                     FGGAMRLPRLKDPVLVSGTDGVGTKIEIARRMGRLDTIGIDLVAMSVNDVSACGAVPL
                     FFLDYLVVGKVVPEQVAEIVAGVDEGCVRAQCALLGGETAEHPGQFPDGDFDMAGFAV
                     GIAERDELWGPHLVREGDVLVGIDSTGLHSNGFSLIRHLLREHTIDLASNFIGAGGGV
                     GLCITPGEGFGPCYDPRSTVGDVLLTPTAIYSPVLHDLGHAGGVHAAAHITGGGFPDN
                     VGRAVPDDLAAVIDLGAWTPSPLFGWLHGLGVHRPELLRTFNCGLGMVLAVDPAHAEK
                     NVAVIEKGNFAARVVGTVVGRDGGDAVRYVGELRV"
     gene            10259..11260
                     /locus_tag="VFZ86_13690"
     CDS             10259..11260
                     /locus_tag="VFZ86_13690"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HEX5643370.1"
                     /translation="MRPVDLIGCGALNLDLIYRLPRSFPLWDELGPPGTEQMMDARVF
                     KAVAEALANVDPVRAGGGQAANTVNALARLGYAAAMVGRVGADEDGRFLLAELAPGDS
                     RLVHREGETGRVYVLLDEDGERRNLVWPAANDAFSPADLPKRLPRTRFALFSSFAGDG
                     PLEAQLALLERLPAGVDVAFDPGEIYARKGVKRFLPILQRCAYLFSTETELEMLCGLS
                     LPESLDFLLNAGVGLVIVKMGGRGARLVGRRVDLYVPPLPAEVVDVTGAGDLFAAGFL
                     AAMIEQAGLERAGRLAAWAASRGISGLGRSAYPDAAAWRERLAEERGEPVVRPGSGW"
     gene            11257..12066
                     /locus_tag="VFZ86_13695"
     CDS             11257..12066
                     /locus_tag="VFZ86_13695"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HEX5643371.1"
                     /translation="MRIGWFSSGRDQAASNLLADVVARAQQDELPLDIGAVFSDRERG
                     EAPESDRFLDLVQRLGLPVVTLSSQASWAEAQKLGVSRADWRREYHRGVAELLVPYNL
                     GVLVMAGYMLIASPSLCRRFAILNLHPALPGGPKGTWQQVIWELLEDEAGETGAMIHL
                     ATAQLDRGPVVSTFRFSLRGPDWDPLWAQFRAKRATMTVQEIAAAEGEAEPLFAEIRR
                     RGEIREIPLLYRTLRLFAEGRLNTANGCVFAESTRLPIDLTEEVDAEVAGR"
     gene            12063..13157
                     /locus_tag="VFZ86_13700"
     CDS             12063..13157
                     /locus_tag="VFZ86_13700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643372.1"
                     /translation="MSAGPAAPAGPGPAGAEAGRIYVTDCEGPLTRNDNAQEIAERFV
                     PDGAEFFARLSKYDDFLADVVRKPGYNAGDTLRLLPPFLRAHYVTDADVEEFSAEQVL
                     LVPQALKTLGAVSALMPAYIISTSYTPYLRALCELAGFPFEHVRCTELSLDAWHMPET
                     EAAWLREVAARVLARPVIEIPDGARSADDLAPEHRETVAELDDLFWTRMEDKVSGAML
                     AAVHPVGGGQKLAALEEIVAAEGVAGGGVMYVGDSITDAPPLEAVKAWGGVSLSFNGN
                     GYAIAAAEFAAASPDAEVQAQLAMAFAAGGRETVEAAVRAWPKGKQGAPARGRARATV
                     GIVAEARDALARASAAARRSVRGERVARLG"
     gene            13167..14021
                     /locus_tag="VFZ86_13705"
     CDS             13167..14021
                     /locus_tag="VFZ86_13705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5643373.1"
                     /translation="MTTPPDPVAPGAAAAATPALQLAGVTVWEWDARAGRRQPILTDV
                     AWTVAPGEQWAVLGPNGAGKSTLLDVAAGMRHPSRGEVAILGHTVGRVDLRWLRERIG
                     HVDVKTEEAFSPRRTALDVVLTGATGTIQVLEDRLDTEARRRAAKLLEQFGCAAVARH
                     AFAACSQGERRRVLLARALMRRPPLLLLDEPADGLDLPGREALLAALALLAGEQPAPA
                     VVLVTHHLEELPPTITHALLLKSGRVVACGAAPAVLADAPLSACFGVPVTVSRAGGRW
                     SARATPSW"
     gene            14731..15384
                     /locus_tag="VFZ86_13710"
     CDS             14731..15384
                     /locus_tag="VFZ86_13710"
                     /inference="COORDINATES: protein motif:HMM:NF014906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5643374.1"
                     /translation="MPVAEEAAPRRAALTQERIVALALEIIDEDGLEAMNMRRLAADA
                     GVKPMSLYHHFPNKQAILDAVGEKIAAAALGASSPHPRWQGRVRQLFLGLHALTEAHP
                     RALPLISTAVVRTPSGRRWMEELMRTLLEAGFSEDRAATVYHVLGSYTLGLGFARMLG
                     DEVQTTDIVAQLVGHWADYPSMMRVGMRLAVWDRPGEFETGLDVLLAHFADRPGGRV"
     gene            15399..>16576
                     /locus_tag="VFZ86_13715"
     CDS             15399..>16576
                     /locus_tag="VFZ86_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012595446.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HEX5643375.1"
                     /translation="MRVKMISPAKTAADSRYWRSIKYSLFPPLGLATLAGYLDPDDEV
                     DLQDEHVEDLRLDDEPDLVVIEAYITSAGRAYRYADHYRARGAYVCLGGLHPTSLPEE
                     AARHADSVFCGPGEDTWPAFLRDFRAGRPQPLYRSTERTLAGLPPARRDLIKRERYLV
                     PNSLVVSRGCPHLCDFCYKEAFFRGGRSFYVQAVEDALAEIDRLPGRHLYFLDDNLFG
                     SPPFAAALFGGLRGMGRVWQAAGTVEAVLRPGLLEKAVESGLRSLFIGFETLDEANLL
                     GAGKTQNLAGAGRRGPASRGESGARPAVGLPQYDAAVRRLHDLGVMVNGAFVFGMDHD
                     DASVFARTVEWAVSRGIETATFHIMTPYPGTRLARRLAAEGRVTSHDWDLYDTRHAVF
                     T"
BASE COUNT         2424 a         5941 c         5777 g         2434 t
ORIGIN      
        1 acggtcaaga aggtgacgtt cgacctccag ccggcgcacc acgaggccga gaaggcgctg
       61 cacgagcacg cgatgacgca agctctgggc gccggagcgg ccggctgatc ccggaggtcc
      121 aagcaccacc gtgcaggtga cggtcgcccg gacgaggccc ttcgcctcgt ccgggcgacc
      181 gctctggaat cggggtcgat gggacggatg aacgcccaaa gagcgtcgcc gaaagagaag
      241 ggcagagcgt ctgttcccgc ggcaggaagc gtggcgacgc cggtaggcga agccgctgcc
      301 ataccgcacc ccggtcggtt caagactgac acagcggagg atggagcggc atgagccttc
      361 tcaagggcag acggtatttc gaccggcgga gcgtcgggcg cgatgccgct gggggcctca
      421 cgctgggcat cgagagcatc cccgacgcca tggcctccgg cgtgctggcc ggggtcaacc
      481 cgatctacgc cctctatgcc gtcatgctgg ccacgcccgt cggcgcgctg ttctcgagct
      541 ctgtgttcat gagcgtgcag acgaccagcg ccatgtcgct gctcgtcgcc gacgtccccc
      601 aggtccatgg aggcgccgat gcggccggcg ccctgttcat gctcacgctc atgaccggcg
      661 tgatcatgct cgccatgggc ctgctcaagc tcggcagcta cctgcgcttc gtcccgaatg
      721 cggtgctcac cgggttcatc aacggcgtag ccgtcctcat catcctgggc cagctcggcg
      781 aattgaccgg cacctccccg gagggcggca acaaggtgga acaggccggg gacctgctgc
      841 gcaactggca ggacatcgac ccgcgggcgt tcgcggtagg cctgaccgcg atcgtcctca
      901 tcgtcgccct gcagaacacg cgtctgaaga gctggggcat ggtcgtcgcc ctggtgatgg
      961 catcgctcct cgtgcccctc ttcaattggg gggacgtggc cctggtcacc gacgtcgcca
     1021 gcatccccga ctcgctgccg ctgccgacgc ttcccgactt ctcggctctc ccggctctga
     1081 tcgtcccggc gatcgcgctg gccttcgtgg gtctcgtcca aggatcgggc atcagccaga
     1141 gcttcacgaa ccccgacgga cgatacccat cggcatcggg cgacttcgtc ggccaggggg
     1201 ccgccaatgt cgcggcgtcg ctcttccagg gcatgccggt cggcggctcg ctctcggcta
     1261 cggcactggt ccacaacgcg ggggtccgct cgcgtgcggc caacatcatc gccggcgtca
     1321 ccatcgcggt cgtgctcctg ttgttcagtg gcctcgtggg gaagctggcc atgccggcgc
     1381 tggccggcct gctcatcgtc gtgggcttcc agacactgaa gccgcatcag atcgacctgg
     1441 tcgtgaggac gggctggctg caggcggtcg tcgtggtgac cacgttcgtc ctgaccctgc
     1501 tcgttcccct gcagtacgcc gttctgatcg gggtcgccgt ctccatcctc ctgcactggg
     1561 tcaagcagtc gaatcagatg gtgctcaagg aatgggtctt cccgccgggt caggcgctgc
     1621 cgcgggagca ggatcctccg gatcggctcc ccggagatca cgtcaccgtc atcacgccgt
     1681 acggcaggat gttcttcgcc actgcgcggc tcatggagga gcagctgccc gaggtcacca
     1741 cagacagccg ccgagccgtg ctcatcctca atctgcgcga gcacgacgaa ctggggagca
     1801 cgttcctcgg gatgttcgat cgctacgcgg aaagcctgcg cgagcacggc gccaagctca
     1861 tgctggccga ggtgggaccc cacctctacg gccagctcca caacaccggg cgattgcgcg
     1921 cgctgaagcc ccgcaacgtg ctcaggcgct cggacacggt ggacgcttcg atcgtcgagg
     1981 cgatcgacac cgctgacgag tggatcgaag cggcggcccg cgaggtggcg gaggcgggcg
     2041 ccgagaatgg caccggcccg gagcatccgg cggaaacgac gtgatccgcg tccggcactg
     2101 caggcgcaac ggcgccggca gcgcgacagc ctgccggcaa atgcgttttg catagttacc
     2161 aaacggtcac gtagagtggc gagtgcccgg aagcgccggg aaacccgtag gaggagagca
     2221 atgaacagca agtgggttgg ttgggccgcg accggcggca tcctgatggt gatcgtcggc
     2281 ggcttcaagg cgatctcggg cctcatcggc ctgttcaacg acgagtggct ggtgcgcggc
     2341 ttcgacgcct actacttcgt cgacatcagc gcgctcgcct ggtggtacct gctcgtgggg
     2401 ttgttcctgc tcttcgccgg cctcgccgtg ctgaacggac gggcgtgggg acgctgggtc
     2461 ggcgtgttcg ccgccggcgt cgcgatcatc tcggagctcc tgtggctacc gatctatccc
     2521 gtctggtcga tcctcctgat caccatgtac gtgttcatga tgatcggcct catcgcggtg
     2581 gggtcggaag actagccgta ccgttcgcgt cgtcagtcgc tctggcgtgg tgcggcgcgg
     2641 gagggggaaa gagagagccc ctcacccgcg ccgcaccact gctcgtagac gctgtgagcg
     2701 cggcactgct ccgaagacag aaaggcagcc atggccgaac cgaagaagat cgcgcacccc
     2761 agcgtcgagg agcgcacggc gcacggcaag gcggcgcgca aggcggtctc cccctccagc
     2821 cacgccggct gggagccggc ggcggaccgt cccgaccccg tcgcgctcct ccaggagcag
     2881 aacgcccggc gtgaacccga cctcgtcccg gtgcggcacg ggcgcatgat ggtctctccg
     2941 ttcaccttct accgtggcgc cgcgcggatc atggccgcgg acctcaagga aacgccacgg
     3001 gcgggcctca cggtgcaact ctgcggcgac gcgcacctct ccaacttcgg catcttcgcg
     3061 tcgccggagc ggcagctcgt cttcgacctc aacgacttcg acgagacgct gccgggcccg
     3121 ttcgagtacg acgtcgcgcg cctggcggcc agcttcacga tcgccgcgcg caacaacggc
     3181 ttcggcaagg cggacgaaca gagcgcgaca cgtgcggtcg tgcgcgccta ccgtgaggcc
     3241 atggccgggt tcgcgcggat gcgcgtcatg gacctctggt acgcacggat caccgaggaa
     3301 gacctgatgg cggccctgcg ctccatggcc cctgagcccg cgccgttcac gaagaaggcg
     3361 acggcgcggg cgggcaagca ggggaagaag gccaagcggc aggcgaggga ggcggacgcg
     3421 aggcgggcga cggagaaggc gggcgagctg cgcatccaga aggccgccga caaagcccgc
     3481 acgcgagaca gcctgcaggc tctctccaag ctcgccgagt acgtcgacgg cggctaccgg
     3541 atcgtcagcc agccgcctat cgtcgtcccg gcgcgcgacc tgctcgagac ctacggcatc
     3601 tcccccgacg aggtccacca cgtcatccac caggagtacc gggcctaccg cgcgacgctg
     3661 cagtcagacc gccggcacct gctcgagaag ttcgagctgg tggacgtggc gcgcaaggtc
     3721 gtcggggtcg gcagcgtcgg gacgcgcgcg ttcatcgtct tgctcctggg ccgcgacgag
     3781 caggatccgc tcttcctgca ggtcaaggag gcgacgtact cggtgctgga ggaccacctg
     3841 ccgaagagcc gtcaccagcc cggctcgcgc gtggtgcacg gccagcggat gatgcaggcc
     3901 gcgagcgaca tcttcctggg ctggaccaag ggcgtgcagg agggccgctt cctgtactgg
     3961 cggcaactgc gcgacatgaa gggctcggcc gacatcgagt cactccagcc gttcggcctc
     4021 gagtactacg cccgcgagtg tggatggaca ctcgcgcggg cgcatgcacg ctcgggagac
     4081 ccggtcgcga tctccgccta cctcgggaag agcgacaagt tcgatacggc gatgaccgac
     4141 ttctccgcac gctacgcgga ccagaacgag cgcgactacg aggcgttcct ggaggcgatc
     4201 cgctccggac ggctcgaggc catcgagggc ctctgacgca cggcgagacc tcttgaccgg
     4261 gtcgccacct gtgtcccggg gtcgctgagt accgctccgg gcggcggctg ctgtcgcatc
     4321 tcccttaccg ctgggttacc atgcgccgac ggcaatcgct ccacgaccca cgatgggaga
     4381 tggcatgacg agcgagcagg gagctccgac cacggccccg gccgcgacgg ggcaggacgc
     4441 gcacaaggcc gccgcggcgg gcgcgaccag ccgctgggcc gtggccgcct gggcgagcga
     4501 catcggcaag tggagcggga tcatcatcct cgtcatcctc ctggtcgtcg tcgtgttctt
     4561 ccttctcgcg gcgaccaggg tgctgctcct cgcggcgctg ttcgccgtcc tcttcgcggg
     4621 cacgttcctc cccatggtgg actggctcca gaagcaccac gtcaagcggg ggctgggggc
     4681 ggtcatcgtg tctgccctcc tcatcctgct cggcatcgcc gtcggcgcgc tcatcgtcta
     4741 cagcgtcatc aagcagctgc cgaccgtcga ggcccggctc gacgacgcgt ggacggagat
     4801 ccagaaggcg ctcacgtcca cgagtgtctc gcaggagcag atcgactcgc tcaagtcgaa
     4861 cgtccagaac ctggccaagg acgcggcgag cgggctcgcc ggcacggtcg tcgacctctt
     4921 cggcggcgtc gccagcctca tcttcggcgc gttcatcagc ctcaacatcc tcgtctggtc
     4981 gctcatccag gggcgcaagc tggcccggtg ggcgtccaag cggatggggc cggtgccgcc
     5041 ggcggtgggc tacgagatct tcgccaacag cgcgcggttc ttccgcggct acctcttcgg
     5101 aagcaccctc gtcggcctct tcaacggcgc cgtgatgggc atcggggcgc tgatcatcgg
     5161 ggtgccgctc gcggggacca tcttcgtggt cggctggttc accaattaca tcccgttctt
     5221 cggcgcgatc gtcagcggcg cgttcgccgt cctgatcgcg ctcggggcgg gcgggccggc
     5281 catggccatc ccgatgctca tcatcgtgat cgtctcgaac ggctatctgc agacgatcgt
     5341 ctcgcagttc gcgctcgggt cggcgctcaa gctgcacccg ctggcggtgc tcttcgcgac
     5401 gaccgccggc agcatgctgt tcggggcggt gggcggcgtg ttcgcggcgc cgttcctcaa
     5461 gatcttcctc gacgcgcgcc tgaagatgaa ggcggccggg ctgttctcga gctcgccgac
     5521 cgccggctcg gcggcggcgg gagcgccgcc gagcgaccag ggtgcggggc cgccggggac
     5581 tgcggagtcg ctgccggccg aggcctgagc cggtcgatcc gccgcttggt cagtcctcgt
     5641 cggccgaggc gccgcgcagc cagcgccacg tgagccgcag cacgatgagg aagctcagga
     5701 tcagcgagag gacgaagccg acgggggcga tgcgctcgag gaggttccag atcgtgcctt
     5761 cgagctccac cgtcgcgatg ccgtaggagg cgacggccga cccgaccagc atgccgacga
     5821 gcatgacggc gatgacgatc tcgcgcccga ggcgcgtgag ccgggtgacc tccttggaga
     5881 gctgcgacgt gtcgacagtg acctcgaacc ggcccttctg gtactggtcg agccacttcg
     5941 tcgtcgcctc gctgagcgtc gggatgcgct ccaccacctg gccggccacg cccatcagct
     6001 gcttcttggc ctcgtcgacg atcttctccc cggagacggc ctcgagcgcc atgtccctta
     6061 gcaggggcac gccctccgag agcaggccgc tgtcggtgga gagcgctcgc gcgacgctct
     6121 ccgtctgcac gagcgccttg accgccaggg tgaggtccgc gtccagccgc aggccgtgga
     6181 tgcgcagcag ctcgaagccc tcgttcgcca cttctccgaa cgaggcgacg cggccacgac
     6241 tcatgtagcg gcccatgcgg cgcgcgaagt cgcgcctgaa ggccttctcg tcgacgtctc
     6301 ccttgaacgg cgtgctcatg ccgcggagcg cgtcggccat gccctcgacg ctgccctgct
     6361 gcgcgacgat catgagctgg ccgagccgca gccgcttctg caggtcgagc tcgcccatca
     6421 tcccgagatc gatgaacgtg aggacgccgg tgtcgagatt gagcagcagg ttgccggggt
     6481 gcgggtcggc gtggaagaaa ccgtccaccg ggagctgctt gatgagcgcc cggagcgcgt
     6541 tgcgggcgat gaggtcccgg tcgagcccgg ccgcctcgat ggccgcgacg tcgctcacct
     6601 tgacgccgac gacgaactcc tgggtgatca ccctggaggt ggagaggtcg gcgtagatgc
     6661 gcggcacgtg gatgccgggg agcccctgca tgttctcggc gaggcgcatg gcgttgtagg
     6721 cctcgccgcg gtagtcgagc tcctcgagca cgcccgacga gaactgctcg agcatgccgg
     6781 agatgtcgag cgagcggacc gtctccgacc tcgtcgaggc gacccgcgcc gcgttcagca
     6841 ggatgccgag gtcggcgcgg atcttgatct ggatgtccgg gcgctgcacc ttgaccgcga
     6901 ccagcgtgcc gtcgtgcagc gtggcgcggt ggacctgcgc ggtggaggcg gccgcgaacg
     6961 gctcgggcgc gaacgacgca tacagctcct caggcgggcc gtcgagctcc tcgatgagga
     7021 tctcgcgcac ctggtccgac gggaacggcg gcacggcgtc ctgcagccga gacagctccg
     7081 tcgccatgtc gaccgggagc acggacgcct ggctcgagac gatctggccg atcttcacgt
     7141 aggtcggccc gagctcctcg agcatgagcc gcagcttgac ggcggccggc ggctcctccc
     7201 agcccctggg catgcgccac acccagctct gcatcgcgtg ccggaacccg cccaggaagg
     7261 cggtccggtc caggacggtg tcgtagccgt aacggagcag cgtgttgtag acctgctgga
     7321 ggcgcaggtc ttcgcggagc ttgtccagcg acgaccgcct catgacggct cctcctcggg
     7381 cgcttcatcg tcggacttcg ccgtcatctc cttcatcttc tcctggccct tgacggcgac
     7441 gtccagcagc tcgcgctcga ggtgtccggg cttggcgcgc gccatctcct cgcgcagcgc
     7501 gtccgggcca ccctggacga tgggcggggt gagaccgaac aggttctcga agaggccgat
     7561 caggaggccg ctcaccagcc cggcgacgag cgcccacagg atgtgctcca cctggaagcg
     7621 ctcggggaag atccaggaga gtatccacac gacgatcgtg ttgatgagga cgatgaccag
     7681 cccgtaggag acgaagatca gggggagcag cagcagctga agcaacggct tgacgaacgt
     7741 catgaggagc ccgaagaccg cgctgatgat gagccacgac agcacccggt actcgccggt
     7801 catgaacacg tgaggcacga tgaggacggt gagcgccagg cagagcgcgc tcaccagcca
     7861 gcgcagcagc agggggcgca cgcggaagtg caggctcacg tgtcgcttcg gggtcgtgac
     7921 ggccataggt tccttctcag ggtgtggtcg tccatttcaa gcagtgcgga cggacacctg
     7981 catgggaagg accgccggcc gggcaaggga cggtgcggcg acgcccggct gcactacacg
     8041 ccgcggcctc gcagcctctc agcgaaaggc cgcgggcgcg tccatgaccg cctgcctcag
     8101 gatccggcga gcgttgacgg acttgacgtg cgcgccgtcg tagaacgcgt ccctgctgcc
     8161 gtggaagctc gcgatgtcgg tgtagtcgag caggtggaag cggtacgtgc cccgcaggct
     8221 cttcaggtac gtcttgagcg cctgctcctt ggcgccccac ccgaccgcgc ggaacgccgc
     8281 cagcgcggtc gggtggtacg gcatgatcac cagcaccggc tccacgtcgt ggagattgaa
     8341 cagctggagg gtgcgctcga aataccgcct cgcgcgagcg ctgtcgtagg cggccggcgc
     8401 cccggcgcga gggacgagtg cggagagata gccggcgagc gtctgctcga atgggatccc
     8461 acgctcgagc cgcgcgtcgt aagagttgta gacgaggctg ccgcgagcgg tgaaccggtt
     8521 gtccgggctc aactcgcggc cctcccggct caccgagatc ccgcgctgct tcgccagcag
     8581 gtcctccggc aggaaccggc tgaggcgcgg ttccgcgagc aggcccgggt ggaacggcgc
     8641 gtccgacagc gtggtggcct gcagcgccca gaagcagcgc agcttgacgc cgggcgcgcg
     8701 ccagaagaga tgccgggcca tggcgtagac atcctcggga cggctgttct gcacggcgaa
     8761 gttgaacgcc ggcaggccgg tgagctcctc ggcgagggtg ggctcgaacc gctgcgcgcg
     8821 cgagcccccg aagatcacga gctccggcgg ctccggaagc cgttccacga ggtcgcactt
     8881 gtagccgcgg tcgtagagcg gcgaggccgc cgcgccgtag gtggcggcgg ccacctcccg
     8941 cggcgcgccg caaccggcca gcgccagcgc ggcggccaag acggccggcg ccagcgcgac
     9001 cgcgccggcg accacccaca tgccccttct gatccctccg tgccgcatag ccggatggta
     9061 cgtcgccggg ggcgtccacg aaaccttcgc ggcgcactgg atgccgggcg gaggcaggac
     9121 gtagactgtg cggaaccgga cggggagcgg tggtggcgga cgagcacggg ttcacgtaca
     9181 agaaagccgg cgtcgacgtg gaggcgggca accgcgccgt cgagctcatg aagcggcgcc
     9241 tcaggagcac gcagcacccg ctcgtcctcg gcggcctcgg cgggttcggc ggggcgatgc
     9301 gcctgccgcg tctcaaggac ccggtgctgg tgtccggcac cgacggcgtg ggcaccaaga
     9361 tcgagatcgc tcggcgcatg ggccgcctcg acaccatcgg catcgacctc gtcgcgatga
     9421 gcgtcaacga cgtctccgct tgcggggcgg taccgctctt cttcctcgac tacctggtgg
     9481 tcggcaaggt cgtgcccgag caggtcgccg agatcgtcgc cggagtggac gaaggctgcg
     9541 tgcgcgccca gtgcgcgctg ctcggcggcg agaccgccga gcaccccggc cagttccccg
     9601 acggcgactt cgacatggcc ggcttcgccg tcggcatcgc cgagcgcgac gagctctggg
     9661 ggcctcatct ggtccgggag ggcgacgtcc tcgtcggcat cgactccacc gggctccaca
     9721 gcaacggctt cagcctcatc cgccacctgc tccgggaaca caccatcgac ctcgccagca
     9781 acttcatcgg cgcgggcggg ggcgtgggcc tctgcatcac gcccggcgag gggttcggcc
     9841 cctgctacga tcccaggtcc accgtcggcg acgtgctgct cacgccgacg gcgatctaca
     9901 gccccgtgct gcacgacctc ggtcacgccg gcggcgtgca cgcggcggcg cacatcaccg
     9961 gcggcggctt ccccgacaac gtcgggcgcg ccgtgccgga cgacctcgcc gccgtcatcg
    10021 acctcggcgc ctggacaccg agcccgctgt tcggctggct ccacgggctc ggcgtgcacc
    10081 gtcccgagct gctcaggacc ttcaactgcg ggctcggcat ggtcctggcc gtggaccccg
    10141 cgcacgcgga gaagaacgtc gccgtcatcg agaagggcaa cttcgccgcg cgcgtcgtcg
    10201 gaacggtcgt cggccgcgac ggcggagacg cggtccgcta cgtcggggag ctccgcgtat
    10261 gaggccggtc gacctgatcg gctgcggcgc cctcaacctc gatctgatct accggctccc
    10321 ccgcagcttc ccgctctggg acgagttggg gccgccgggc accgagcaga tgatggacgc
    10381 ccgcgtgttc aaggcggtgg ccgaagccct cgccaacgtc gaccccgtgc gcgccggcgg
    10441 cggccaggcg gccaacacgg tcaacgcgct cgcgcgcctc gggtacgcgg cggcgatggt
    10501 cggccgggtg ggcgccgacg aggacggccg cttccttctt gccgagctgg ccccgggcga
    10561 ctcccggctg gtgcaccgcg agggagagac cggccgcgtc tacgtgctgc tggacgagga
    10621 cggcgagcgg cgcaacctcg tgtggccggc ggccaacgac gccttctcgc cggccgacct
    10681 gcccaagaga ctgccgcgca cccgcttcgc tctcttctcg agcttcgccg gcgacgggcc
    10741 gctcgaggcc cagctggcgc tgctcgagcg gctgcccgcc ggcgtcgacg tcgccttcga
    10801 tcccggcgag atctacgcgc gcaagggcgt caagcgcttt ctgccgatcc tgcagcgctg
    10861 cgcctacctc ttctcgacgg agacggagct cgagatgctc tgcggcctct cgctgccgga
    10921 gtccctcgac ttcctgctca acgccggcgt cgggctcgtc atcgtcaaga tgggcggccg
    10981 cggggcgcgc ctcgtcggcc gccgcgtcga cctgtacgtg ccgccgctgc cggcggaggt
    11041 ggtggacgtc accggcgccg gcgacctctt cgcggcgggg ttcctcgccg ccatgatcga
    11101 gcaggccggg ctcgagcggg ccggccggct cgccgcctgg gcggcgagcc gcggcatctc
    11161 cggcctcggc cgcagcgcct acccggacgc cgccgcctgg cgcgagcgtc tcgccgaaga
    11221 gcgcggcgag cccgtcgtcc ggccggggag cggctggtga ggataggctg gttctccagt
    11281 ggccgcgacc aggcggcctc caacctgctg gcggacgtcg tcgcccgcgc gcagcaggac
    11341 gagctgcccc tcgacatcgg cgccgtcttc agcgaccgcg agcgcggcga ggcgcccgag
    11401 agcgaccgct tcctcgacct ggtgcagcgc ctcggcctgc ccgtcgtcac cctgtccagc
    11461 caggccagct gggccgaggc gcagaaactc ggcgtctcgc gcgccgactg gcgcagggag
    11521 taccaccgcg gcgtcgccga gctgctcgtg ccgtacaacc tcggcgtgct ggtgatggcc
    11581 gggtacatgc tcatcgccag cccgtccctc tgccgccggt tcgccatcct caaccttcat
    11641 cccgcgctcc cgggagggcc aaagggcacg tggcagcagg tcatctggga actgctcgaa
    11701 gacgaggccg gcgagacggg cgcgatgatc cacctcgcca ccgcccagct ggaccggggt
    11761 cccgtggtca gcaccttccg cttctccctg cgggggccgg actgggaccc gctctgggcg
    11821 cagttccgcg ccaagcgcgc gacgatgacc gtgcaggaga tcgccgccgc ggagggcgag
    11881 gccgagccgc tgttcgccga gatccgccgc cgcggcgaga tccgcgagat cccgctgctc
    11941 taccgcaccc tgcggctgtt cgcggagggc cggctgaaca cggccaacgg ctgcgtgttc
    12001 gccgagtcca cccggctgcc gatcgacctc acggaggagg tcgacgcgga ggtggccggc
    12061 cgatgagcgc cggcccggcc gcgccggccg gcccgggccc cgcaggggcc gaggccggcc
    12121 gcatctacgt caccgactgc gagggcccgc tcacccgcaa cgacaacgcc caggagatcg
    12181 cggagcgctt cgtcccggac ggcgccgagt tcttcgcccg gctcagcaag tacgacgact
    12241 tcctcgccga cgtggtgcgc aagcccggct acaacgccgg cgacacgctg cgcctgctcc
    12301 cgccgttcct gcgcgcccac tacgtcaccg acgcggacgt cgaggagttc agcgccgagc
    12361 aggtgctgct cgtgccgcag gcgctcaaga cgctcggcgc cgtgagcgca ctcatgcccg
    12421 cctacatcat ctcgacctcg tacaccccgt acctgcgcgc gctctgcgag ctcgccgggt
    12481 tccccttcga gcacgtgcgc tgcacggagc tgagcctcga cgcctggcac atgcccgaga
    12541 cggaggcggc gtggctgcgc gaggtggccg cgcgggtgct ggcgcgcccc gtcatcgaga
    12601 tcccggacgg ggcgcgttcc gcggacgacc tcgctcccga gcaccgggag acggtcgccg
    12661 agctcgacga tctcttctgg acgcgcatgg aggacaaggt gagcggcgcg atgctggccg
    12721 ccgtgcaccc cgtcggcggc ggccagaagc tcgccgctct cgaggagatc gtcgccgccg
    12781 agggcgtggc gggcggcggc gtcatgtacg tcggggactc gatcacggac gcgccgcccc
    12841 tcgaggccgt caaggcctgg ggcggcgtct cgctgagctt caacggcaac ggctacgcca
    12901 tcgccgcggc ggagttcgcc gccgcgagcc cggacgccga ggtgcaggca cagctggcga
    12961 tggcgttcgc ggccggcggc cgggagacgg tagaggccgc cgtccgcgcc tggcccaagg
    13021 gcaagcaggg cgccccggcc cgcggcaggg cgcgcgccac cgtgggcatc gtcgccgagg
    13081 cgcgcgatgc gctcgctcga gcctccgccg ccgcgcggcg cagcgtccgc ggcgagcggg
    13141 tcgcgcgcct cggctgacgc tccctgatga ccaccccgcc cgatccggtc gcccccggcg
    13201 ccgccgccgc cgccaccccc gccctgcagc tcgccggcgt caccgtgtgg gagtgggacg
    13261 cgcgcgcagg ccggcgccag ccgatcctca cggacgtcgc ctggaccgtc gcccccggcg
    13321 agcagtgggc cgtgctcggt cccaacggcg ccggcaagtc gaccctgctg gacgtcgccg
    13381 cgggcatgcg ccaccccagc cgcggcgagg tcgcgatcct cggccacacc gtgggacgcg
    13441 tggacctgcg ctggctgcgc gagcgcatcg gtcacgtgga cgtgaagacc gaggaggcct
    13501 tctcgccgcg ccgcacggcc ctcgacgtcg tcctcaccgg cgcgaccggg acgatccagg
    13561 tgctggagga tcgcctcgac accgaggcac ggaggcgggc ggcgaagctt ctggagcagt
    13621 tcggctgcgc cgccgtcgcg cggcacgcct tcgcggcgtg ttcgcagggc gagcggcgac
    13681 gcgtcctgct ggcgcgcgcc ctgatgcgac gccccccgct cctcctgctc gacgagccgg
    13741 ccgacgggct cgacctgccg ggccgggagg cgctgctcgc ggcgctcgcc ctgctcgccg
    13801 gcgagcagcc cgccccggcc gtcgtcctcg tgacgcatca cctcgaggag ttgcccccga
    13861 cgatcacgca cgccctgctg ctcaagagcg ggcgcgtggt cgcctgcggc gccgcgcccg
    13921 ccgtcctcgc cgatgcgccg ctcagcgcct gcttcggcgt gccggtgacc gtatcgcgcg
    13981 cgggcggccg ttggtccgcg cgcgcgaccc cgagctggta gctcccgctc gcggcctccc
    14041 gcgcgtggcc gccaaggcgc cgccgctccc gtcgtcggtt tcccccgccg gctcctctgt
    14101 tcacgtatct acaatgtcga gacacagggg gctgtcccgc ccatcgccgc tctgcacgtc
    14161 tctccattgt agaaacgccc agagaggacc ccgaccagga caccccccat gccccatcct
    14221 ctgccgggca ccccgggtcc gcgtcccagc ggatggcctc aacgtcaacg ccacccgccc
    14281 gtcgacgccc tgcggctggg ccgagcctcc cctaccatcg atccacagtc cgtcctcaag
    14341 gagacccagt gactcgggag ttcagcgccg tcgtgcagcg cgaaggcgac atcttcgtcg
    14401 tccagtgtct cgacgtggac gtcgcgagtc agggcgcctc ggaggaagag gcactggcaa
    14461 acctccagga ggctctggag ttgtacgtcc acgccgcagg ccagcgatgc tccgcgtgtg
    14521 agcaaggtcc gcgtcgacat cgatgccgct taggccactc cccgacaggt cgagcgtcgt
    14581 ctcaaggcag ccggcttcgt ggcggtcgcc agtcacggca gtcacccgct gaaccatacg
    14641 cgcgtacgtt gaccagcgcc gggcgctccc cgtaccgtac tttgtaagcc ttacgcagta
    14701 tggttcgggt accctgacct ggaggacgcc atgccagtcg ccgaggaggc cgcgccgaga
    14761 cgcgccgcgc tcactcagga gcgcatcgtc gcactcgccc tcgagatcat cgacgaggac
    14821 ggcctggagg ccatgaacat gcgccggctc gcggcggacg ccggcgtcaa gcccatgagt
    14881 ctctaccacc atttccccaa caagcaggcg atcctggacg cggtgggcga gaagatcgcc
    14941 gcggcggcgc tcggagcgtc ctcgccgcac ccccgctggc agggccgtgt gcgccagctc
    15001 tttctggggc tgcacgcgct caccgaggcg cacccgcgcg ccctgccgct catctcgacg
    15061 gccgtggtgc gcaccccgag cggccgccgc tggatggaag agctgatgcg cacgctgctc
    15121 gaggccggct tcagcgagga ccgggccgcc accgtgtacc acgtgctcgg ctcgtacacg
    15181 ctgggcctgg gcttcgcgcg catgctgggc gacgaggtgc agaccaccga catcgtcgcc
    15241 cagctcgtcg gccactgggc ggattacccg agcatgatgc gcgtcggcat gcggctcgcg
    15301 gtgtgggacc ggccgggcga gttcgagacc ggcctggacg tgctgctcgc gcacttcgcc
    15361 gaccgtccgg gagggcgcgt gtgaggcgcg atggccccct gcgggtcaag atgatcagcc
    15421 ccgccaagac ggcggccgac agccggtact ggcgctcgat caagtactcc ctgttcccgc
    15481 cactcggcct cgccaccctg gcgggctacc tcgaccccga cgacgaggtc gacctgcagg
    15541 acgagcacgt cgaggatctg cgactcgacg acgagcccga cctcgtcgtc atcgaggcct
    15601 acatcacctc ggccggccgc gcctaccggt acgccgacca ctaccgcgcc aggggcgcct
    15661 acgtctgcct cggcggcctg cacccgacga gcctccccga ggaggcggcc cggcacgccg
    15721 acagcgtgtt ctgcgggccg ggcgaagaca cgtggccggc gttcctgcgc gacttccgcg
    15781 ccggccggcc ccagccgctg taccgctcga ccgagcggac cctcgccggc ctgccgcccg
    15841 cgcggcgcga cctcatcaag cgcgagcggt acctggtgcc gaactcgctg gtggtaagcc
    15901 gcggctgccc gcacctctgc gacttctgct acaaggaggc gttcttccgc ggcggcaggt
    15961 cgttctacgt gcaggcggtc gaggatgctc tcgccgagat cgaccgcctc cccggccgcc
    16021 acctctactt cctcgacgac aacctcttcg gcagcccgcc gttcgccgcg gcgctgttcg
    16081 gcggcctaag ggggatgggc cgcgtctggc aggcggcggg cacggtcgag gccgtcctac
    16141 ggcccggcct cctcgagaag gcggtcgaga gcgggctgcg cagcctcttc atcggcttcg
    16201 agacgctgga cgaggcgaac ctgctgggcg ccggcaagac gcagaacctg gccggcgccg
    16261 ggcggcgcgg gcctgcgagc cgcggagaga gcggagctcg gcccgcggtc ggactcccgc
    16321 agtacgacgc cgccgtccgt cgcctgcacg acctcggcgt catggtcaac ggcgccttcg
    16381 tcttcggcat ggaccacgac gacgcgtcgg tctttgcccg gaccgtggag tgggcggtca
    16441 gccgcggcat cgagacggcg acgttccaca tcatgacccc gtacccgggc acgcgactcg
    16501 cacggcggct cgccgccgag ggccgcgtca cgagccatga ctgggacctg tacgacacgc
    16561 gccacgccgt gttcac
//
LOCUS       DASUMX010000289         9487 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1546095, whole genome shotgun sequence.
ACCESSION   DASUMX010000289 DASUMX010000000
VERSION     DASUMX010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 9487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9487
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1546095"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="VFZ86_13720"
     CDS             complement(<1..285)
                     /locus_tag="VFZ86_13720"
                     /inference="COORDINATES: protein motif:HMM:NF013396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HEX5643376.1"
                     /translation="MKLSELVQGVSNASVRGDPATEITGLAYHTRDVTDGTLFFCVPG
                     LSFDGHAFAAAAVEAGAAALVCERDTGVGAPQVVVPSVRRAMALAAARWYG"
     gene            complement(333..2063)
                     /locus_tag="VFZ86_13725"
     CDS             complement(333..2063)
                     /locus_tag="VFZ86_13725"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX5643377.1"
                     /translation="MAAPSTGRRRPSGRRGGLLGSPKRAGNGCDGRIRLLRFLFIVFL
                     VLVGGKAVALASSSQDLTQLAQEQQLQEVVLPAPRGSILDRNGNELAVGKPQQTVYAT
                     PYLLDDPQAAAEELCDALQINRRRDRRDVEEALSDGESGFAYVARKVDPELARAALKL
                     DLPGVGSYAEEERTYPLKGTAAQVVGYAGMDNIGLFGIELAYEEELSGVAGSETVVRD
                     PAGRPLKTVAQVAPQSGENVYLTLDREIQYYAEDVLEKTVRHAAAKAATAIVMDPRTG
                     EVLVMANVFRQGFHGFGKEDEARKNRAVEDAPEPGSIFKLVTISGALADGTVKPSTTF
                     SVPWKVPVADRTIQDSHVHATEEYSVREIMQYSSNVGAYKIADEMGKAGLYKWVKAFG
                     FGEQTGIEFPVESTGLVRPVEEWWDSSIGNIPMGQGVGATAIQMASAFSTVAYNGWQV
                     KPRLVAQVGTEVHDSVEKHRVIPARVARQVRDMLRLAVEEGTGGEARIPGYDVAGKTG
                     TAEIPLKDRSGYAKDIYTSSFIGMVPAGRPRLVVLVTVESTPGFGGVVAAPAFKEITS
                     FALQHLEIAP"
     gene            complement(2063..2488)
                     /locus_tag="VFZ86_13730"
     CDS             complement(2063..2488)
                     /locus_tag="VFZ86_13730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643378.1"
                     /translation="MAAAARHAHATAPAPRREAEARPEAPAYAYRAPHTRRKAAQRSR
                     LTRTFVIFVACLTVLAVGRVALSFAVVQKSLQTGAVVREERRISEQNGELREEVARLS
                     STSRIRQIAERELGLVEATHFAHPDALLPAGAFEVAATR"
     gene            complement(2547..3518)
                     /gene="rsmH"
                     /locus_tag="VFZ86_13735"
     CDS             complement(2547..3518)
                     /gene="rsmH"
                     /locus_tag="VFZ86_13735"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEX5643379.1"
                     /translation="MLPQTSMQLREYEMALAHTPVLLDELLELLRPARGETAVDCTFG
                     AGGHAEAVAAALGARGRLIAIDRDPSVAEYFRDVALAAPCACELYHGNFAEVLAEREP
                     ASADVVYMDLGVSSMQLDRRERGFSYAYDAPLDMRMDPELEVTAADLVNTLPEAELTG
                     IFRRYGEERYARNIARGVVQARKKAPLATTSELVEIVKRTIPTPARFGAGNPARRVFQ
                     ALRIVVNDELGSLARGLEEAFRVLRPGGRLAAISFHSLEDRMVKEYFKGHAAGCICPP
                     GLPQCVCGHEPTMRVLTRKPVTPRAAEAESNPRSTSAKLRVAVKLED"
     gene            complement(3487..3948)
                     /locus_tag="VFZ86_13740"
     CDS             complement(3487..3948)
                     /locus_tag="VFZ86_13740"
                     /inference="COORDINATES: protein motif:HMM:NF014439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643380.1"
                     /translation="MQRPLLVGEFEVTLDAKNRIAVPARLRAAFDEGIYVTQENERCL
                     GGYSPEEFQRRLEEDVDVTRAGSVERRDVKRRLAATAVYFPELDRQGRLTLPAKHLAY
                     ADVAKDAAAPRAVTIIGVADHVEIWDRAAWTDYRAHLEEEADASADEHATS"
     gene            complement(4195..4497)
                     /locus_tag="VFZ86_13745"
     CDS             complement(4195..4497)
                     /locus_tag="VFZ86_13745"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HEX5643381.1"
                     /translation="MAARIAAPVVFLIAVIALVGIVVNSGVMDEPAPTPTVEATKTKD
                     GSVTVTKRYVVQEGDSLSSIAVRFNTTTAELKALNPDLSGSTVVIGQRIVVPRPAD"
     gene            complement(4764..5678)
                     /gene="pyrF"
                     /locus_tag="VFZ86_13750"
     CDS             complement(4764..5678)
                     /gene="pyrF"
                     /locus_tag="VFZ86_13750"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR02127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEX5643382.1"
                     /translation="MQHFSDRLATAIRTRRSVVCVGLDPVLERMPPELVEKYRPQAAE
                     LGEDAAVAACFQEFCSGVIDAVADVAACVKPQAAFFEQYGAAGWRALGAVIRCAHDHE
                     LPVILDVKRGDIASTGAAYGHAAFGGAAGFAGPATGIGADAVTASPYLGDDSLQPLVD
                     HCATGHGVFVLTRTSNPGASLLQEVEVDGRPLFLRVADLVRELGSAHIGEAGYSDVGA
                     VAGATAPAQLRAVREVLPNAFLLVPGYGAQGAGAGALAGVAAGDAAGFVVNASRSIIF
                     AWQAAGGDYRRAAAAAAESMRSDLQDTL"
     gene            complement(5750..6268)
                     /locus_tag="VFZ86_13755"
     CDS             complement(5750..6268)
                     /locus_tag="VFZ86_13755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643383.1"
                     /translation="MLLGLIEDSYLHTGTFTQFAAEVLQVIAYAVTVPVAFTEGFLKS
                     YLSIDRVVEDDEGRISIIPRAGASIEDMIRDVDEITAQVDPGAVFNPVVRARLDAVHQ
                     TSSRAMKWDLDTLSGMDDAQLMQVLREMQAESEAAPEPGADEATDYVRAKIATIRRNV
                     GRFQPRHEGDVA"
     gene            6498..7319
                     /gene="otsB"
                     /locus_tag="VFZ86_13760"
     CDS             6498..7319
                     /gene="otsB"
                     /locus_tag="VFZ86_13760"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HEX5643384.1"
                     /translation="MSGADALDGFMNAAGGAPDAAALVCDIDGTISPIAPTPADALVP
                     AAFRELLAALTQRLGLVVFATGRALEDGHRMIPLQGAAYVGTHGLETMTPDGSVSVDP
                     QAERYVPLIRAVAEGAARDLDCEALGVVLEDKRTVLAVHYRLARDAAATRHEILKRVI
                     EPARERGLAIATGHFAFEVRPPLPFTKGSAVRRLLAAGDYLSAIGCGDDLTDITVFTA
                     VRDWGERDARRRTLAVAAVTDETPRSVLEAADVLVRATPGVHEVLARLARAVGAV"
     gene            complement(7383..8156)
                     /locus_tag="VFZ86_13765"
     CDS             complement(7383..8156)
                     /locus_tag="VFZ86_13765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX5643385.1"
                     /translation="MAVEVVREGAVAIVTVNRPEALNALNTETNEALLAAVRELAPDD
                     GVRSVVLTGGGDKSFVAGADIAEMSVLTAEEARRFGALGQAVMSGIEAAPQPWIAAVN
                     GFALGGGCELALACDIRLAAENAKFGQPEINLGITPGFGGTQRLPRNVGEGWAKYLVL
                     SGRFIRADEALRIGLVQAVVPKDELMTQAMKLAEELAGKSPLAMRYCKAAVNAAADTD
                     IATGQGIERDLFALAFATEDQTEGMAAFLEKRAPEFGGR"
     gene            8341..8880
                     /locus_tag="VFZ86_13770"
     CDS             8341..8880
                     /locus_tag="VFZ86_13770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643386.1"
                     /translation="MSEHSRRGHVRPARVPSAARSVPFLTALICAALLAACGGSSGGA
                     TDAPDASAGPPTPVPSPRITAGKPPAAAVDVVREFWTLVGTGRLEEAQRSLVAPGSPI
                     RQWNGEDIASARFVRVVPGSVSRGAVDEATVEFAAIVWIEPSPGVSPWGEPGEHQLFE
                     HVVRMSDGTWRMWDSGTGP"
     gene            complement(8890..>9487)
                     /locus_tag="VFZ86_13775"
     CDS             complement(8890..>9487)
                     /locus_tag="VFZ86_13775"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEX5643387.1"
                     /translation="PVVFISGMEEGVFPHQRSLDEQNVEEERRLAYVGITRAMDRLYL
                     VHARARTLWGAAQYNIPSRFLDEIPTELTERQTVGGRPTSWDSGGGWGGESRGRGAGG
                     GWDGGGWSGRRPRAEDEADHETLGTGRRITGLEKKQLDEEVVEQFFAPGDRVLHATLG
                     EGTVLAMQSGGIVLIRFENDGSERRLMASVAPLKKLRS"
BASE COUNT         1267 a         3401 c         3430 g         1389 t
ORIGIN      
        1 gccgtaccag cgcgccgccg ccagcgccat cgcgcggcgc accgacggga cgacgacctg
       61 cggcgcgccg accccagtgt cgcgctcgca cacgagcgcc gcggcgccgg cctccacggc
      121 cgcggcggcg aacgcgtgac catcgaacga aagcccggga acgcaaaaga aaagcgtgcc
      181 gtcggtgacg tcacgcgtgt ggtaggcgag acccgtgatc tcggtggcgg ggtcgccgcg
      241 caccgacgcg ttcgagaccc cttggaccag ctcgctcagc ttcatcggcg cggattctag
      301 cagacccgcc gccccctcgc ggcgacccgt gttcacggcg cgatctcgag gtgctggagg
      361 gcgaagctgg tgatctcctt gaacgccgga gccgccacga cgccgccaaa ccccggtgtc
      421 gactccaccg tgacgaggac gacgaggcgc ggtcggccgg ccggcaccat cccgatgaac
      481 gaggacgtat agatgtcctt ggcgtagccg ctgcggtcct tcagcgggat ctcagccgtg
      541 cccgtcttgc cggcgacatc gtagcctggg atgcgggctt ccccgcccgt cccctcttca
      601 acggccaggc gcagcatgtc gcgcacctga cgcgcgaccc tggcggggat gacgcgatgc
      661 ttctcgaccg agtcgtgcac ctccgtcccc acctgggcga cgagcctcgg cttcacctgc
      721 cagccgttgt aggcgacggt cgagaacgcc gaggccatct ggatcgcggt cgcgcccacg
      781 ccctgcccca tcgggatgtt gccgatagag gagtcccacc actcctccac cgggcggacg
      841 agccctgtgg actcgacggg gaattcgatg ccggtctgct cgccgaagcc gaaggccttg
      901 acccacttgt agagaccggc cttacccatc tcatcggcga tcttgtaggc gcccacgttg
      961 ctggagtact gcatgatctc gcggacggag tactcctcgg tggcgtgaac gtgggagtcc
     1021 tggatcgtgc ggtcggccac agggaccttc cagggcacgc tgaacgtcgt gctcggcttc
     1081 acggtgccgt cggcgagcgc cccggagatg gtcaccagct tgaagatgga gccgggctcc
     1141 ggggcgtcct cgacggcacg gttcttccgg gcttcgtctt ccttgccgaa gccgtggaat
     1201 ccctgccgaa acacgttggc catgacgagc acttcgccgg tgcgcgggtc catcacgatc
     1261 gcggtggccg ccttggccgc ggcgtggcgc acggtcttct cgaggacgtc ctcggcgtag
     1321 tactggatct cgcggtcgag cgtgagatag acattctcgc cggattgcgg cgcgacctgg
     1381 gcgacggtct tgagagggcg tcccgcgggg tcacgcacga cggtctcgct gccggccacg
     1441 ccggacagtt cctcctcgta cgccagttcg atgccgaaca gcccgatgtt gtccatgccg
     1501 gcgtagccga cgacctgggc cgccgtgccc ttgagcgggt acgtccgctc ctcttcggcg
     1561 tagctgccca cgcctggcag gtcgagcttg agcgccgccc tggcgagctc ggggtcgacc
     1621 ttgcgcgcca cgtaggcgaa gccgctctca ccgtccgaga gcgcctcttc cacgtcgcgg
     1681 cggtcgcgcc ggcggttgat ctgcagcgcg tcgcagagct cctcggccgc ggcttgggga
     1741 tcgtccagaa gatagggcgt ggcgtacacc gtctgctgcg gcttgcccac ggccagttcg
     1801 ttgccgttcc tgtcgaggat ggaaccgcga ggggccggaa gcacgacctc ctgcagctgc
     1861 tgctcctggg cgagctgggt gaggtcctgc gacgaggagg cgagcgcgac cgccttgccg
     1921 ccgacgagca ccaggaagac gatgaagaga aagcggagga gacggatgcg cccgtcgcag
     1981 ccgttgccgg cgcgcttcgg gctcccgagg aggccgccgc gccggccgga cgggcgacgg
     2041 cgtcctgtcg agggagccgc catcagcggg tcgccgcgac ctcgaaggcg cccgcgggca
     2101 gaagtgcgtc aggatgcgcg aagtgggtcg cctccacgag ccccagctca cgctcggcga
     2161 tctggcggat gcgcgacgtc gaggaaagcc gcgccacctc ctcgcggagc tcgccgttct
     2221 gctcgctgat ccggcgttcc tcgcgcacga ccgcgccggt ctgcaggctc ttctgcacca
     2281 cggcgaagct gagcgcgacg cgcccgacgg cgagcacggt gaggcaggcg acgaagatca
     2341 cgaacgtccg cgtcaggcgt gagcgctgcg ccgccttgcg ccgcgtgtgc ggcgcgcggt
     2401 aggcgtaggc cggggcctcc ggccgcgcct cggcttccct gcgtggggct ggggccgtag
     2461 cgtgagcgtg tcgagcggca gcggccatca gtccgccccg cgcttggccc tcacgggccg
     2521 cgagtcgctc aacagcacta cgtccatcaa tcttcgagct tcacggcgac gcgaagcttg
     2581 gccgaggtgc tgcgcgggtt ggactcggct tcggccgcgc gtggcgtcac gggcttgcgc
     2641 gtgagcacgc gcatggtcgg ctcgtgaccg cacacgcact gcggcaggcc cggcgggcag
     2701 atgcagcccg ccgcgtggcc cttgaagtac tctttgacca tccggtcctc gagcgagtgg
     2761 aagctgatgg ccgcgaggcg gccgcccgga cgcagcaccc tgaaggcctc ctcgagaccg
     2821 cgggcgagcg agcccagctc gtcgttgacg acgatgcgca gcgcctggaa gacgcggcgg
     2881 gccgggttgc cggcgccgaa gcgggcgggc gtcgggatcg tgcgcttgac gatctcgacc
     2941 agctcgctcg tcgtcgccag cggagccttc ttacgcgcct gcacgacgcc gcgggcgatg
     3001 ttgcgcgcgt agcgctcctc gccgtagcgg cggaagatgc cggtgagttc tgcctccggc
     3061 agcgtgttca ccaggtccgc cgccgtgacc tcgagctcgg ggtccatgcg catgtccagc
     3121 ggcgcgtcgt aggcgtagct gaagccgcgc tcgcgccggt cgagctgcat cgacgagacg
     3181 ccgaggtcca tgtagacgac gtcggccgag gccggctccc gctcggcgag cacctcggcg
     3241 aagttgccgt ggtacagctc gcaggcgcat ggggcggcga gggcgacgtc gcggaagtac
     3301 tcggcgacgc tcgggtcgcg gtcgatggcg atcaggcgtc ccctggcgcc gagcgcggcg
     3361 gccacggcct cggcgtggcc ccccgcgccg aaggtacagt cgacggccgt ctctcccctg
     3421 gccgggcgca gcagctcgag cagttcgtcg agaaggaccg gggtgtgagc gagggccatc
     3481 tcgtactcac gaagttgcat gctcgtctgc ggaagcatcg gcctcctcct caaggtgggc
     3541 ccggtagtct gtccaggcgg cgcggtccca gatctccacg tggtcggcga cgccgatgat
     3601 ggtcaccgcc cgcggggcgg ccgcatcttt cgcgacgtcg gcgtaggcga ggtgcttggc
     3661 gggcagcgtc agccggccct gcctgtcgag ctcggggaag tacaccgccg tcgcagccag
     3721 ccggcgcttg acgtcgcggc gctcgaccga ccccgccctg gtcacgtcca cgtcctcctc
     3781 gagccggcgc tggaactcct cggggctgta gccgccgagg cagcgctcgt tctcctgggt
     3841 gacgtagatg ccttcgtcga aggcggcgcg caggcgggcg gggacggcga tgcggttctt
     3901 cgcatcgagc gtgacttcga actcccccac cagtagcggc cgctgcatgg gcgccactct
     3961 aacccaccaa tccccattac gcaacacttt tctccctcaa ttccccactt cgtggggatc
     4021 agccctcagc aacgccctcc gaccggtcct ggggacggat gaggcgaggg agccgcgagg
     4081 gagcggcgag gctcggagca acatgcgcag cgggcgcggg caggcgccgg ccgagggtgg
     4141 tggcgtgtgg ggagaccgcg ggtggcgccc gggcggcggg cggcgccggc gccctcagtc
     4201 cgctgggcgg gggaccacga tcctctgccc gatcacgacg gtgctgccgc tgaggtcggg
     4261 gttcagcgcc ttgagctccg ccgtggtggt attgaagcgg acggcgatgc tcgagagcga
     4321 gtcgccttcc tgcacaacgt acctcttggt gaccgtcaca ctgccatcct tggtcttggt
     4381 cgcctcgacg gtcggtgtgg gcgccggctc gtccatgacg ccggagttca cgacgatgcc
     4441 gacgagggcg atcacggcga tgaggaacac caccggcgcc gcgatacgcg ccgccatccg
     4501 ggaggagcgc gagccggcgg cggaccgcgg cggacgcgcc cgcggcgcac gcttggcgcc
     4561 gctcacaggg cgcggcgggc ggctggcggg cggctccacg aacgggtcga cgaccggatc
     4621 ccggggcagc ggagccgggc ctgcctgcgc gggcggcgtg cccgcagtcc ccagggattc
     4681 caggcgccgg agcgcggcgt cgggatcgtc gggatcaagc ggaggctgac cggccgcagc
     4741 cctctcctct ggtgtctcgt cggtcacagg gtgtcctgga ggtcgctgcg catagattcg
     4801 gcggccgccg ccgcagccct tcgatagtcg ccgccggcgg cctgccaggc gaagatgatg
     4861 gaacgggacg cgttgaccac gaagccggcg gcgtccccgg cggcgactcc cgcgagagcg
     4921 ccggcgccgg cgccctgggc gccgtagccg ggcaccagca ggaacgcgtt gggcagcacc
     4981 tcgcgcaccg cgcggagctg ggccggcgcg gtcgcgccgg cgaccgcgcc cacgtcgctg
     5041 tagccggcct cgccgatgtg ggcggagccg agctcgcgca ccagatcggc gacgcgcagg
     5101 aacagcggcc gtccgtccac ctccacctcc tgcagcagcg aggcgcccgg gttgctcgtg
     5161 cgcgtgagga cgaagacgcc gtggccggtg gcgcagtggt cgaccagcgg ctgcagtgag
     5221 tcgtcgccca gataggggct ggcggtgacg gcgtcggcgc cgatgccggt ggccgggccg
     5281 gcgaagccgg cggcgccgcc gaacgcggcg tgcccgtagg ccgcgccggt ggacgcgatg
     5341 tcgccgcgct tgacgtccag gatcaccggc aactcgtggt cgtgcgcaca tctgatgacg
     5401 gcgccgagcg cgcgccagcc ggcggcgccg tactgctcga agaaggccgc ctgcggcttg
     5461 acgcacgcgg cgacgtcggc gacggcgtcg atgacgccgc tgcagaactc ctggaagcag
     5521 gcggcgacgg cggcgtcctc gcccagctcc gccgcctgcg gccggtactt ctcgaccagc
     5581 tccggcggca tgcgttcgag cacagggtcg agcccgacgc agacgacgct gcgacgcgtg
     5641 cggatggccg tggccagtct gtcggagaag tgctgcacgg aaggcccttc ggtcgtgcgc
     5701 gcaatggtat cgcacggggc ccggcgccga cagcgccggg cggccgggct caggccacgt
     5761 cgccttcgtg acgcggctgg aaccggccga cgttgcggcg gatcgtggcg atcttggcgc
     5821 gcacgtagtc ggtggcctcg tccgcccccg gctcgggcgc cgcctcggac tcggcctgca
     5881 tctcgcgcag cacctgcatg agctgcgcgt cgtccatgcc cgacagggtg tcgaggtccc
     5941 acttcatcgc cctgctcgag gtctggtgca cggcgtccag cctggcgcgc acgaccgggt
     6001 tgaacacggc gccggggtcc acctgagcgg tgatctcgtc cacgtcgcgg atcatgtcct
     6061 cgatgctggc tccggccctg gggatgatgc tgatgcgacc ctcgtcgtcc tccaccacgc
     6121 gatcgatgga caggtacgac ttgaggaacc cctcggtgaa cgccaccggc acggtcaccg
     6181 cgtaggcgat cacctggagc acctcggccg cgaactgcgt gaacgtcccc gtgtgcaggt
     6241 agctgtcctc gatcaagccc aggagcatgg acttgagata ggggtccgcg cgcgtgtcgc
     6301 gagccctgcc gccccggagg gggcgacgtg cgctgtcggc cgggtcactc atctctctcg
     6361 gcccgggtat cggcacgccg ccgggggaac ttgacgaggg acgtaccacg atgagactcg
     6421 ttgtcgaccg gcctcgggcg ggggtagggt gagaggcaag cgaggcggga ggaagcgtgg
     6481 agactgggtc gccgatcatg agcggggccg atgccctgga cggtttcatg aacgccgccg
     6541 ggggagcgcc ggacgcggcg gcgctcgtct gcgacatcga cgggaccatc tcccccatcg
     6601 cccccactcc cgccgacgcg ctcgtgccgg ccgcctttcg ggagcttctc gccgctctga
     6661 cgcagcgcct cgggctggtc gtcttcgcca ccggccgcgc gctcgaggac ggccaccgca
     6721 tgatcccgct gcaaggcgct gcctacgtcg gcacccacgg gctcgagacg atgacccccg
     6781 acggctccgt cagcgtggat ccgcaggcgg agcgctacgt ccccctgatc cgcgccgtcg
     6841 ccgagggcgc cgcgagagac ctcgactgcg aggccctggg ggtcgtgctc gaggacaagc
     6901 ggaccgtcct cgccgtccac taccgcctgg cgcgggacgc cgccgccacg cggcacgaga
     6961 tcctgaagcg ggtgatcgag cccgcgcgcg agcgcgggct cgccatcgcc accggccact
     7021 tcgccttcga ggtgcgaccg ccgctcccgt tcaccaaagg gtccgccgtc cggcggcttc
     7081 tcgcggccgg cgactacctc tcggcgatcg gctgcggcga cgacctcacc gacatcacgg
     7141 tgttcaccgc cgtgcgcgac tggggcgagc gggacgcacg gcggcgcacg ctggccgtcg
     7201 cggcggtcac cgacgagacg ccgcgatccg tgctggaggc tgccgatgtg ctggtgcgcg
     7261 ccacgcccgg cgtccacgag gtgctggcgc ggctggccag ggccgtcggc gccgtctgac
     7321 gagcccgggc cgcggcgccg gccgggagcg acggcggagc ggcgggcggc gccggcggcg
     7381 cttcagcgcc ccccgaactc cggcgcccgc ttctcgagga acgcggccat gccctccgtc
     7441 tggtcctcgg tcgcgaacgc cagcgcgaag aggtccctct cgatcccctg cccggtcgcg
     7501 atgtcggtgt cggcggcggc gttgacggcg gccttgcagt agcgcatcgc cagcgggctc
     7561 ttgccggcga gctcctccgc cagcttcatc gcctgggtca tgagctcgtc cttggggacc
     7621 acggcctgga cgagcccgat gcgcagggcc tcgtcggcgc ggatgaagcg cccgctgagc
     7681 accaggtact tggcccagcc ctcgcccacg ttgcgcggca gtcgctgcgt gccgccgaag
     7741 cccggcgtga tgcccaggtt gatctcgggc tggccgaact tggcgttctc cgcggccagc
     7801 ctgatgtcgc aggcgagggc cagctcgcaa ccgccgccga gggcgaagcc gttgacggcg
     7861 gcgatccacg gctggggcgc cgcctcgatg ccgctcatga cggcctgacc cagcgcgccg
     7921 aagcggcgcg cctcttcggc cgtcagcacg ctcatctcgg cgatgtcggc gccggcgacg
     7981 aacgacttgt cgcctccgcc ggtgagcacg acggagcgca cgccgtcgtc gggcgcgagc
     8041 tcgcgcacgg cagcgagcaa cgcctcgttg gtctccgtgt tcagcgcgtt gagcgcctcc
     8101 gggcggttga cggtgacgat ggcgacggcg ccttcgcgga cgacctcgac ggccatggcg
     8161 accctcctcg gaccatggga ctggactggc tgcgccccac tctaccgcag gggcgtccgc
     8221 ggccggtcgg ccccgacccg cccgagagtc gtcaacctcg tctgcgcatc gtcaacctgt
     8281 acacccggcc actcccgtga tgaagtgcgt gcgggcgcgt cacgacacga ggaggcagcc
     8341 ttgagcgagc acagtcgccg cggtcacgtc cgtccggcac gcgtcccgag cgcggcgcgc
     8401 tccgtgccgt tcctcacggc gctgatctgc gccgccctgc tcgccgcctg cggcggctcc
     8461 tccggcggcg cgacggacgc cccggacgca tccgccggcc cgccgacacc cgtcccttct
     8521 ccgcggatca ccgccggcaa gccgcccgcc gcggcggtgg acgtcgtgag ggagttctgg
     8581 accctcgtcg gcacggggcg cctggaggag gcgcagcggt cccttgtcgc ccccggctcc
     8641 ccgatccgac agtggaacgg agaggacatc gcctctgcgc gtttcgtgcg tgtcgtgccg
     8701 gggtcggtct cccgtggggc cgtcgacgag gcgaccgtcg agttcgcggc gatcgtgtgg
     8761 atcgagccgt cgcccggagt gtcgccctgg ggcgagccgg gcgagcatca gctgttcgag
     8821 cacgtcgtcc gcatgtccga cggcacgtgg cgcatgtggg acagcggcac cggaccgtga
     8881 cggctccgct caggagcgca gcttcttcag cggcgccacg ctggccatga gccgccgctc
     8941 cgagccgtcg ttctcgaagc ggatgagcac gatcccgccg ctctgcatgg ccaggacggt
     9001 gccctcgccg agggtcgcgt ggagcacgcg gtcgccgggg gcgaagaact gctccacgac
     9061 ctcctcgtcg agctgcttct tctcgagccc ggtgattcga cggccggtcc cgagcgtctc
     9121 gtggtcggcc tcgtcctctg cacggggccg ccggccgctc cagccgccgc cgtcccagcc
     9181 gccgccggcg ccgcggcccc gcgactcgcc gccccagccg ccgccggagt cccagctggt
     9241 cggcctgccg ccgacggtct ggcgctctgt gagttccgtg gggatctcgt cgaggaagcg
     9301 tgacgggatg ttgtactggg cggctcccca gagcgtcctc gcgcgcgcgt gcaccaggta
     9361 cagccggtcc atcgcgcgcg tgatgcccac gtacgcgagc cgccgctcct cttccacgtt
     9421 ctgctcgtcc agcgaccgct ggtgcgggaa cacgccctcc tccatgccgg agatgaacac
     9481 caccggg
//
LOCUS       DASUMX010000290         6267 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1547726, whole genome shotgun sequence.
ACCESSION   DASUMX010000290 DASUMX010000000
VERSION     DASUMX010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 6267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6267
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1547726"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1392)
                     /locus_tag="VFZ86_13780"
     CDS             complement(<1..1392)
                     /locus_tag="VFZ86_13780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5643388.1"
                     /translation="MSHYGPSRELVVTVCRTLLDGGYLKATEGNVSVRVSGEDAYAIT
                     PSSYDYGRMEPGDICVLGLDGRRILGAKKASIEASMHAAVYERRPDVSVIIHTHQPYA
                     SALALMERPIPALFDEQVRYLGRSVELVPYAPSGTSFLKKNVAKKLASGANAYILENH
                     GVLVLGGDAEQAVHNMALLEKVALDYLLALLTDGRAEKVPLPIREIAFAKLRKDEKRL
                     AAQVEEARAAREAAEAGVEKAAGATARAAAGGPATAAAAVPAGPGVPSGAQLAGYAIS
                     RYPDVAEVYRRLDELVSRPLRPIRRDAMDGYLDWFDAECRRSRELTDQAAELIPGGVQ
                     HNLAFNYPFPLAIEKAEGARLWDVDGNEYIDFLQAGGPTVLGSNYEPVRRQVAEVVEQ
                     SGPVTGLFHKYELKLAELVNRLMPACEMFRMLGSGTESVMAAVRAARAFTGKKWVVKV
                     GGAYHGWSDQMVYG"
     gene            complement(1397..1768)
                     /locus_tag="VFZ86_13785"
     CDS             complement(1397..1768)
                     /locus_tag="VFZ86_13785"
                     /inference="COORDINATES: protein motif:HMM:NF014687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HEX5643389.1"
                     /translation="MGEPVRPGDDLRDSAPRRTWLAAERTYLAWLRTGLGALGLALAV
                     GRLLPALIDASHLAFGVLGGGFAVLGVFLLVLAAYRAQRTRTALKAGIPLPTDFWAVW
                     VLTVASCVLAVGTIVLVIAEV"
     gene            1910..2335
                     /locus_tag="VFZ86_13790"
     CDS             1910..2335
                     /locus_tag="VFZ86_13790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643390.1"
                     /translation="MNTFSAQTPTTRRGGSPAWTLRHPVTSTVAVLVCAVLIGALIGV
                     LISAPLTGTETATLHRAGGHALATVAQAQHATVATVAAVTGGEIASPSHIRGTRSPAR
                     GGFAVGRASGAGSGFIVIAPGTRAPVRGGFAGRSIGAGG"
     gene            complement(2470..3597)
                     /locus_tag="VFZ86_13795"
     CDS             complement(2470..3597)
                     /locus_tag="VFZ86_13795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HEX5643391.1"
                     /translation="MHIAPFAVEQWMNAHETTATWNLGETCVHSLTVDELLALTGDAD
                     DVLRRLRDTRLTYGHIIGSHELRAQIAALYGGRIGADDVLVTNGAIGANFLALFALTE
                     PGAGVVCVKPTYQQLYSVPQALGADLRLLPLREKNGYLPDVDELRSLVDDATRLIIVN
                     NPNNPTGALMDEALLREIVAVARERDAWVLCDEVYRMLEHDPGTRPPSIVDLYEKGVG
                     TGSMSKSYSLAGLRTGWVAGPPEALARCLEVRDYTTISCGVLDDILATVTLEHRDAVL
                     ERNLEIVRGNLAIVDEWVEREERLRYARPRAGTTTLIRYDYRVPSLEFCQGMFDLNGA
                     FVVPGAAFDEEYAFRLGYACARDVLEGGLAAVSAYLRTLEA"
     gene            3758..5179
                     /locus_tag="VFZ86_13800"
     CDS             3758..5179
                     /locus_tag="VFZ86_13800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013014000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEX5643392.1"
                     /translation="MDTNEFRQLGHELIEWIAAYREHIADYPVMSPSQPGEVAALLPA
                     EPSLRGGDLGGIVADLDRVVMPGITHWNHPGFLHYFPSNSDLSSVLADLVSSGLGVQG
                     MSWQTSPAATEVEDVVMQWLREMVGLPDTFTGVIEDTASTSTFTALLCARERTSDFSQ
                     NRGGLQAEAAPLVVYASDQAHSSVPKAALLAGFGAEHLRLIGTDHEHALRLDHLEHAI
                     DEDLAAGRRPCAVVAAVGTTATTALDPVAGTATLCERHGMWLHVDAALAGTAMVCPEY
                     RWMWEGVERADSVVFNPHKWMGTGFDLSAYYVRDPEHLVRVMSTDPSYLRTAQDGLVR
                     NLRDWGIPLGRRFRALKLWFLLRSEGVEGLQRRIRRDVGNAAWLAEQVVAAPEWALMA
                     PAPLQTVCLRHVPPALAGDEEALAAHNAAIVRRVTAAGDYYFGTSLLKGRLIIRISVG
                     AQATEREHVAGAWAALQEAATTV"
     gene            complement(5274..>6267)
                     /locus_tag="VFZ86_13805"
     CDS             complement(5274..>6267)
                     /locus_tag="VFZ86_13805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEX5643393.1"
                     /translation="GNTMVVNGKTWPYLNVEQRRYRLRLLNGSQSRFLILKMNNGMPF
                     TQIGSEGGYLPKPVRLRQLLIAPAERADVIVDFSKVRRGSRIILRNVGPDEPFGGGEV
                     GEEFEAADVGTTGQVMQFRVRKATSRDTSARPGRLDLPEIKRLPAASVVRNVSLNELD
                     SATVKASEDPDGNVVLDPDGQPFGPREAALGTWTSTGGPEPLFWMDGVTENPALGAVE
                     SWNLRNFTMDAHPIHIHQVQFQVVKRVVTDPMMSPHGMRGQGAVLKPEPWETGWKDTV
                     IVYPGELTRVKARFTIAGLFVWHCHILEHEDNEMMRPFRVGPAPPTAALTTQPQ"
BASE COUNT          870 a         2294 c         2212 g          891 t
ORIGIN      
        1 cccgtacacc atctggtcgc tccagccgtg gtacgcgccc ccgaccttga ccacccactt
       61 cttgccggtg aaggcgcggg cggcgcggac ggccgccatg acgctctccg tgccggagcc
      121 gagcatgcgg aacatctcgc aggcgggcat cagccggttc acgagctcgg ccagcttgag
      181 ctcgtacttg tggaagaggc cggtgacggg gcccgactgc tcgacgacct cggcgacctg
      241 ccgccggacc ggctcgtagt tgctgccgag cacggtcggg ccgcccgcct ggaggaagtc
      301 gatgtactcg ttgccgtcga cgtcccagag gcgggccccc tcggccttct cgatcgccag
      361 cgggaacggg tagttgaagg cgaggttgtg ctgcacgccg ccggggatga gctccgccgc
      421 ctggtcggtc agctccctgc tgcggcggca ctccgcgtcg aaccagtcga ggtagccgtc
      481 catcgcgtcg cggcggatgg ggcggagcgg ccgggagaca agctcgtcga ggcgccggta
      541 gacctcggcg acgtccgggt agcgactgat ggcgtagccg gcaagctggg cgccggaggg
      601 gacgccgggg ccggccggca cggcggcggc ggccgtcgcg gggccgcccg cggccgcccg
      661 cgccgtcgct ccggcagcct tctccacgcc cgcctcggcc gcctcgcgcg cggctcgcgc
      721 ctcctccacc tgcgcggcca gccgcttctc gtccttgcgc agcttcgcga acgcgatctc
      781 gcggatcggc agcgggacct tctcggcgcg cccgtcggtg agcagcgcca gcaggtagtc
      841 gagcgcgacc ttctcgagga gggccatgtt gtgcaccgcc tgctcggcgt cgccgccgag
      901 cacgagcacg ccgtggttct cgaggatgta ggcgttggcg ccgctcgcga gcttcttcgc
      961 cacgttcttc ttgagaaagc tcgtccccga cggcgcgtac gggacgagct cgaccgagcg
     1021 gccgaggtag cgcacctgtt cgtcgaacag ggcggggatc ggccgctcca tcagcgccag
     1081 cgcgctcgcg tacggctggt gcgtgtggat gatcacgctc acgtccgggc gcctctcgta
     1141 cacggcggcg tgcatcgacg cctcgatcga ggccttcttc gccccaagga tgcgccgccc
     1201 gtcgagcccg agcacgcaga tgtcgccggg ctccatcctg ccgtagtcgt agctggacgg
     1261 cgtgatcgcg taggcgtcct caccggacac gcggaccgag acgttgccct cggtcgcctt
     1321 gaggtagccc ccgtccagca atgtgcggca gacggtgacg acgagttctc gcgaggggcc
     1381 gtagtgactc actggttcac acctccgcga tgacgaggac gatcgtgccg accgccagca
     1441 cgcagctggc gaccgtgagc acccagacgg cccagaagtc cgtcggcagc ggaatcccgg
     1501 ccttgagagc ggtgcgggta cgttgggccc ggtacgccgc cagcacgagc aggaagacgc
     1561 cgagcacggc gaagcctccg ccgagcacgc cgaaggccag gtgcgaggcg tcgatcagcg
     1621 ccggcagcag cctccccacg gccaaagcca gccccagtgc gcccagcccg gtgcgcagcc
     1681 aggcgaggta ggtgcgctcg gcggccagcc acgtgcggcg cggcgctgaa tcgcgcaggt
     1741 cgtcccctgg tctcaccggc tcacccatct gccgtcattc tatgccgcct ccccgtgccg
     1801 ggcgattcac cccacttggg tgaggcggcc actcatcgcc cttccatagc ctgagcacgc
     1861 agcgccgctg ctcgaaggaa cgcgagacag gcgacacgag gaggaaccca tgaacacgtt
     1921 cagcgcccag acgccaacca cacgacgcgg tgggtcgccc gcgtggacgc tgaggcaccc
     1981 cgtgacgtcg acggtcgcgg tccttgtgtg cgcggtcctg atcggggcgc tcatcggcgt
     2041 cctcatcagc gcaccgctca ccggcacgga gacggcgacg ctgcaccgcg ccggcggtca
     2101 tgccctcgcc accgtcgcgc aggcgcagca cgccaccgtg gcgaccgtcg ccgccgtcac
     2161 cggcggcgag atcgcgtcgc cgtcgcacat ccgtggcacg cgcagtccgg cgcggggcgg
     2221 cttcgccgtc ggccgggcaa gcggagccgg ctccgggttc atcgtcatcg caccgggaac
     2281 gcgcgcaccc gtcaggggcg gcttcgccgg acgctcgatc ggcgccggcg gctgacagga
     2341 tggcccgcgg cgctcacgcc gcgggcaggg accgccgccg caagaacggg atggcagggg
     2401 ccgccgtccc gatggtgggg cggcaggggc cggggcgccg cacggcggcc cggcctcgcc
     2461 gcgcagggct caggcctcga gcgtgcgcag gtaggccgac acggcggcga gcccgccctc
     2521 gaggacgtcg cgggcgcacg cgtagccgag gcggaaggcg tactcctcgt cgaaggccgc
     2581 gccgggcacg acgaaggcgc cgttgaggtc gaacatcccc tggcagaact ccagcgaggg
     2641 gacccgatag tcgtagcgga tgagggtggt ggtgccggcg cgcggccgcg cgtaccgcag
     2701 ccgctcctcc cgctccaccc actcgtccac gatcgccagg ttgccgcgca cgatctcgag
     2761 attgcgctcg agcaccgcgt cgcggtgctc gagcgtgacc gtggcgagga tgtcgtcgag
     2821 cacgccgcac gagatggtcg tgtagtcgcg cacctcgaga cagcgggcca gcgcctccgg
     2881 cgggcccgcc acccagcccg tgcgcagccc cgccagcgag tagctcttgc tcatgctgcc
     2941 cgtgccgacc cccttctcgt agaggtcgac gatcgacggc ggccgcgtcc ccggatcgtg
     3001 ctcgagcatg cggtagacct cgtcgcagag cacccacgcg tcgcgctccc gggccacggc
     3061 gacgatctct cgcagcaacg cctcgtccat cagggcgccg gtggggttgt tggggttgtt
     3121 gacgatgatg agccgggtcg cgtcgtcgac cagcgagcgg agctcgtcca cgtccggcag
     3181 gtagccgttc ttctcgcgca gcggcagaag ccgcaggtcc gcgcccagcg cctgcggcac
     3241 cgagtagagc tgctggtagg tgggcttgac gcacaccacg ccggcgccgg gctcggtgag
     3301 cgcgaacagc gcgaggaagt tggcgccgat cgcgccgttg gtcacgagga cgtcgtcggc
     3361 gccgatgcgc ccgccgtaca gggcggcgat ctgggcgcgg agctcgtgcg agccgatgat
     3421 gtggccgtag gtgaggcggg tgtcgcggag ccggcgcaag acgtcgtccg cgtcgcccgt
     3481 cagcgcgagc agctcgtcga ccgtgagcga gtgcacgcag gtctcgccga ggttccaggt
     3541 ggccgtcgtc tcgtgggcgt tcatccactg ctcgacggcg aacggcgcga tgtgcatgct
     3601 ggcctccagg gtcctgcgag cggcaacccc gcccgcactc gccagcatcc tcccccgaga
     3661 gcggcatccc cggcaaccgg cgccgccccg gcgccggtcg ttcgatagaa tcgcggcgcc
     3721 ggccaccccc cgccgccgca acgacccggg agcgcccatg gacaccaacg agttccggca
     3781 gctcggccac gagctgatcg agtggatcgc cgcctaccgt gagcacatcg ccgactaccc
     3841 cgtcatgagc ccctcgcaac cgggcgaggt ggcggcgctg ctgcccgccg agccttcgct
     3901 gcgcggcggc gacctcggcg gcatcgtcgc cgacctcgac cgggtcgtca tgccgggcat
     3961 cacgcactgg aaccaccccg gcttcctgca ctacttcccc agcaacagcg acctctcgtc
     4021 ggtgctcgcg gatctcgtct cctccgggct cggcgtgcag ggcatgagct ggcagacgag
     4081 ccccgcggcg acggaggtcg aggacgtcgt catgcagtgg ctgcgcgaga tggtgggtct
     4141 gccggacacg ttcaccggcg tgatcgaaga cacggccagc acctcgacct tcaccgcgct
     4201 gctctgcgcc cgcgagcgca cctcggactt ctcgcagaac cgcggcggtc tgcaggcaga
     4261 ggccgcgcct ctcgtcgtct acgcgagcga ccaggcgcac agctcggtgc ccaaggcggc
     4321 gctcctcgcc ggcttcggcg ccgagcacct gcgcctcatc gggaccgacc acgagcacgc
     4381 gctccggctg gaccatctgg agcacgcgat cgacgaagat ctggcggccg gtcgccggcc
     4441 ctgcgccgtg gtcgccgcgg tcggcaccac ggcgaccacg gcgctcgacc cggtggccgg
     4501 caccgcgacc ctctgcgaac gccacggtat gtggctgcac gtcgacgcgg cgctcgcggg
     4561 gacggcgatg gtctgccccg agtaccgctg gatgtgggag ggcgtggagc gcgccgactc
     4621 cgtcgtcttc aacccgcaca agtggatggg caccggcttc gacctctccg cctactacgt
     4681 gcgcgacccc gagcacctgg tgcgcgtgat gagcacggac ccgagctacc tgcgcaccgc
     4741 gcaggacggc ctcgtgcgca acctgcgcga ctggggcatc ccgctgggcc gccgcttccg
     4801 cgcgctcaag ctctggttcc ttctacgctc cgagggcgtc gagggactgc agaggcgcat
     4861 ccgccgcgac gtcggcaacg ccgcctggct cgccgagcag gtggtcgcgg cgccggagtg
     4921 ggcgctcatg gcgccggcgc cgctccagac cgtgtgcctg cggcacgtgc cgccggcgct
     4981 cgccggcgac gaagaagcgc tcgcggcgca caacgccgcg atcgtgcggc gcgtcaccgc
     5041 cgccggcgac tactacttcg gcacctcgct gctcaaaggc cggctcatca tccgcatctc
     5101 cgtgggcgcg caggccaccg agcgtgagca cgtcgccggc gcgtgggcgg cgctccagga
     5161 ggcggcgacc accgtgtgac ccgccggcgg gcccggagca cgcggcagcg tacgcgcgag
     5221 ggccgcgccc gccgaggcgg gcgcggccct cgcccttcgt cactgcagta cggtcactgc
     5281 ggctgcgtcg tgagcgcggc cgtcggcggg gccgggccca cgcggaacgg ccgcatcatc
     5341 tcgttgtcct cgtgctcgag gatgtggcag tgccacacga agaggccggc gatggtgaac
     5401 ctcgccttca cccgggtcag ctcgcccggg tagacgatga cggtgtcctt ccacccggtc
     5461 tcccaaggct cgggcttcag gaccgcgccc tgccccctca tgccgtgcgg cgacatcatc
     5521 gggtcggtga cgacgcgctt gaccacctgg aactgcacct ggtggatgtg gatcgggtgc
     5581 gcgtccatgg tgaagttgcg caggttccag ctctcgacgg ctcccagggc cggattctcg
     5641 gtcacgccgt ccatccagaa cagcggctcg gggccgccgg tgctggtcca ggtgccgagc
     5701 gcggcctccc ttggcccgaa cggctggccg tcggggtcga ggacgacgtt gccatccggg
     5761 tcctcggacg ccttgaccgt cgccgagtcg agctcgttga ggctcacgtt gcggaccacg
     5821 ctcgccgccg gcagccgctt gatctcgggc aggtccagtc tgccgggccg cgcgctcgtg
     5881 tcgcgcgatg tcgccttgcg cacgcggaac tgcatcacct gacccgtggt accgacgtcc
     5941 gccgcctcga actcctcccc gacctcgccg ccgccgaagg gctcgtcggg accgacgttg
     6001 cgcaggatga tgcgcgaccc gcgccggacc ttggagaagt ccacgatcac gtcggcgcgc
     6061 tccgccggcg cgatcagcag ctgcctgagc ctgaccggtt tgggcagata cccgccctcg
     6121 ctgccgatct gggtgaaggg catgccgttg ttcatcttga ggatgaggaa ccgtgactgc
     6181 gagccgttca gtaggcggag gcgataccgc cgctgctcca cgttcaggta cggccaggtc
     6241 ttgccgttca cgaccatcgt gttgccg
//
LOCUS       DASUMX010000291         4246 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_254743, whole genome shotgun sequence.
ACCESSION   DASUMX010000291 DASUMX010000000
VERSION     DASUMX010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4246
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254743"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="VFZ86_13810"
     CDS             <1..613
                     /locus_tag="VFZ86_13810"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HEX5643394.1"
                     /translation="LFPLPVHADPRTAALTTAPLNHRGLFLPGELAHHFGLPAMTGYA
                     GTDDDLPGSWLAAAETAHTVMLAGIVGSELMTTIGLTNRYQLFTPEHMLLDDDLYRRA
                     ANAFRDLELDEEQLALDVIDAVGPGGHFLGQAHTRRHMRETVERSIGQEIGPDGAHLR
                     DPVEVARERGLDILEHYVPEPLDADKAAELARICAAADAELGG"
     gene            complement(636..1316)
                     /locus_tag="VFZ86_13815"
     CDS             complement(636..1316)
                     /locus_tag="VFZ86_13815"
                     /inference="COORDINATES: protein motif:HMM:NF037009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5643395.1"
                     /translation="MTRYELPFSDPTPDLAPTAARILEAAKRVLSREGFRGLTFETIA
                     REAGENPALIRYHFGSKAGLLAALVDSVLYQEAVELIEQLTAVPAGEERRSQLLELHE
                     GLPEDTDAYRNFFELIPHCLRDDELQPHLRGLFDWYRKLDAWALKDAGDKEPPAHIAP
                     MALLTVAIADGIALQKLADPDLDFEPAFALWRRLVLGYVEAGGEPGAAPGEPGGGAES
                     AEAGGKRA"
     gene            1496..2893
                     /gene="mtbB"
                     /locus_tag="VFZ86_13820"
                     /pseudo
     CDS             1496..2893
                     /gene="mtbB"
                     /locus_tag="VFZ86_13820"
                     /inference="COORDINATES: protein motif:HMM:NF021053.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="[dimethylamine--corrinoid protein]
                     Co-methyltransferase"
     gene            2979..4246
                     /locus_tag="VFZ86_13825"
                     /pseudo
     CDS             2979..4246
                     /locus_tag="VFZ86_13825"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
BASE COUNT          666 a         1521 c         1484 g          575 t
ORIGIN      
        1 cctcttccct ctgccggtgc acgccgaccc acggacggcg gcgctgacca cggcgccgct
       61 caaccaccgc ggcctcttcc tccccggcga gctggcgcac cacttcgggc tgccggcgat
      121 gacggggtac gccggcaccg acgacgacct gccgggcagc tggctggccg cggcggagac
      181 ggcgcacacg gtgatgctcg ccggcatcgt cggctccgag ctcatgacga cgatcgggct
      241 caccaaccgc taccagctgt tcacgcccga gcacatgctg ctggacgacg acctctaccg
      301 gcgcgccgcg aacgcgttcc gcgacctcga gctcgacgag gagcagctgg cgctcgacgt
      361 gatcgacgcg gtgggccccg gcgggcactt cctggggcag gcccacaccc gccggcacat
      421 gagggagacc gtggagcgct ccatcggcca ggagatcggc cccgatggag cgcacctgcg
      481 cgaccccgtc gaggtcgcgc gcgagcgcgg gctcgatatc ctcgagcact acgtgccgga
      541 gccgctggac gcggacaagg cggcggagct ggcgaggatc tgcgccgccg ccgacgcgga
      601 gctcggcggc tgaggaggcc gcgggctgcg gcgggtcagg ctctcttgcc gcccgcctcg
      661 gcgctctcgg cgccgccgcc gggctcgccg ggcgcggccc ccggctcgcc tccagcctcg
      721 acgtacccga gcacgagccg gcgccagaga gcgaacgccg gctcgaagtc gaggtcgggg
      781 tcggcgagct tctgcagggc gatgccgtcg gcgatcgcca ccgtcagcag cgccatgggc
      841 gcgatgtggg ccggcggctc cttgtcgccg gcgtccttga gcgcccaggc gtccagcttg
      901 cggtaccagt cgaacaggcc gcgcaggtgc ggctgcagct cgtcgtcacg caggcagtgc
      961 gggatgagct cgaagaagtt gcggtaggcg tccgtgtcct cgggcaggcc ctcgtgcagc
     1021 tcgagcagct gcgagcgccg ctcctcgccg gccgggacgg cggtgagctg ctcgatgagc
     1081 tccaccgcct cctgatacag caccgagtcc acgagcgccg ccaggagccc ggccttgctg
     1141 ccgaagtggt agcggatgag ggccgggttc tccccggcct cccgggcgat ggtctcgaag
     1201 gtgagcccac ggaagccctc gcgcgagagg acgcgcttcg cggcctcgag gatgcgggcg
     1261 gcggtcgggg cgaggtcggg cgtggggtcg ctgaacggca gctcgtacct cgtcaacgcc
     1321 gtcaccccct tcgccgtggt gcggtttccg ctgggcaccc tatccgattc ggccccccgc
     1381 acgccagacc cgacgcgcgt gggcgccttg aacgccccga ggccgtctgg tagtgtaccg
     1441 caaggttagt taggtaacta accaatccca caggacggcc ctcgggggtg ctggagtgga
     1501 gagacctgta cgcacgcggc tcggcgacgg cgggctcgtc gagatgacgc cgtcggagat
     1561 ccgcgaggac ctcctcgcgg gcacggagat aggcgcccgc cgcgccaagg cccctcccct
     1621 ctccgaggcg gagatcgacc acctgctcga catcttcgcc agcgagtcca agttcagcgc
     1681 cgtcgacatg ggcgacgagg tctgcctcag cttcgacggc tcgggcaacc tcgactcggg
     1741 cacgcgcgtc gacgagctct acagctacca gagccaccgc ggcgccgaca tgctcgagct
     1801 gttcgccatc gactactcgt acaaggcgat caagaccatc ctcagctacg agacgcaggc
     1861 gatgaagaac gcccagctca acgtcatcgc gcccctgcag tacggggcga tgccggacct
     1921 cggccgctac tccatgccgg acgggcccat ccccaactgg tccgagctca tgccgcaggg
     1981 ccgcatcgcc gaggcgcggg acgcgcaggt ggcggccatc gaccacgcgg tgagcgacat
     2041 ggtcttcgtc gccgagggca tgtgggaggc cggcacggac ggcatggact tcgacaccgc
     2101 ggccgccgcc ggcgacggcg acttcctcgc caccctcacg gcggtcgaga ccatcaaggg
     2161 tcgccacccg gacatcggca tcgagctggg catggccagc gaatgcgtgc tcggcatgca
     2221 cggcgagctc acctacaagg gcaggcggct cgccgggatg tggccgaagg cgcagctcga
     2281 ggtggcccag gaggccggcg ccaccatctt cgggccggcc gtcaacgtca acaccaccag
     2341 gtcgatcgcc tggaacgtcg cccgggcgtg cacgatcatc aagccctgca tggacgtcgc
     2401 cgagatcccc gtgcacccca acgtgggcat gggtgtcggc ggcgtgccga tgagcccgta
     2461 ccctcccgcg gacgccgtct cgcgcgtctc ccgcgcgctg gtcgacatcc tggggtgcga
     2521 cgggttgtag gtgggcgtgg gtgaccctct gggcatggcg tgcacgcacg cgctggcctc
     2581 gggcatgggt ggcatgcgga ccgccggaga tctggtgggc cgcatgcaga tgacccgggg
     2641 catgcgcctc aagaacgcca agacctacgt ggcggagaag ctcggttgcg ccgtcgccga
     2701 cctcgccgac cccatcgtca tgcacgacgt gcgccgcgac ctcggcctcg ggcggatccc
     2761 ggtgcaggag ctcacgtacg ccacgcacgc cggcgccatg gaggcgaagt tccacatcgc
     2821 cgaggtgctc gacgtgccga tcaacagcgt cacgcgcttc caggagtgcg cagggctgcg
     2881 cgcccgcgcg tgaagattcg ccaacccgat caaggaggag cggcgtgaag cgaaacctct
     2941 tcgccggcgg cacgcgcagc cgcgggctca gcgtcaacgt gttcacggag tccgagctcg
     3001 acgacatcca cctcggcaca ctcgaggtgc tcgagcgcac cggcgtcttc gtcgaggcgg
     3061 acgacgcgcg cgacatcttc aaggacggcg gctgcgtcgt cgacggcgag acgcgcatgg
     3121 tgcgcatccc gccgcacgtc gtcgaggacg cgatcgcggc cgcacccagc aagtacgtcc
     3181 tgtgcggccg cgacccgaag aacgacttcc tcatggagcc gggccgcgtg gcgttcacca
     3241 acttcagcga gggcatcgcc gtcatcgaca tggacaccgg caaggcgcgg gagtccacgc
     3301 tgcaggacgt cgccgacatc gcccggatca acgactacct cgacgagatg gacacgcacg
     3361 aggtctccgt cggggcccgg gacgtcccgc cggagacggc cgcggtccac aacaccgagg
     3421 cgcagctgct caacacgacc aagcccatcg gcatcggccc gctctcgggg gtcgagtgcg
     3481 acgccatctt ccgcatgtgc gccgaggtcg tgggcggcgc ggacgagctg cgcgcgaggc
     3541 ccatcgtgta cggcggcgtc tgccccgtga gcccgctgaa gctcccgcgg gacgccaccg
     3601 aggtgatcat cgagtgctcg cgctggggca tccccgacaa catcctgagc atggcgatgg
     3661 ccggcggctc cagccccgtc acgctcgccg gcaccctggt cacgcacaac gccgaggtgc
     3721 tcgccggcat cacgctcgcg cagctcgtgg agcgcggcag cccggtcatg tacggcagtt
     3781 ccaccacggc gatggacctc aagctcgcgg cggcctccgt cggctcgccc gagatcgcgc
     3841 tgatcagcgc cgcggtggcg cagatggcgc ggcgctacat gcttccgagc ttcgtcgccg
     3901 ggttgtaggc ggactccaag ctcggtgacg cccaggtcgg gcacgagaag accctcaccg
     3961 gactcacggc agcgttggca ggggccaacc tgatctacgg tgcggggatg atcgagtcgg
     4021 gcgtcacctt cgacagcgcg cagttcgtca tggacaacga gatcgcgcgc atgatcaagc
     4081 acatcgtcgg cggcatccgc gtcgacgacg agagcctcgc ggtggacgac atcgccgccg
     4141 tcggggcgtt cggcgacttc ctcagcctcg acgcgaccat gcgccacatg cgcgagctga
     4201 gccagccgga ggtcctcgac cgccgcgtgc gcgaggactg ggaggc
//
LOCUS       DASUMX010000292         6189 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_112027, whole genome shotgun sequence.
ACCESSION   DASUMX010000292 DASUMX010000000
VERSION     DASUMX010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 6189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6189
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112027"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..895
                     /locus_tag="VFZ86_13830"
     CDS             59..895
                     /locus_tag="VFZ86_13830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007779870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX5643396.1"
                     /translation="MSQSFTYTGTSDYIAAPDLMQTVNVAVQLSRPLLIKGEPGTGKT
                     MLAQSIARGLDMPLLVWNVKSTTKAQDGLYVYDTVQRLYDGQFGDRDVSDIARYIKPG
                     KLGEAFASEERVVLLIDEIDKADLEFPNDLLWELDQMEFHIPETHATVKAVHRPIVLI
                     TSNAEKELPDAFLRRCVFHYIEFPDEEMMERIVRVHVPDLDKRLLDLAIQAFYWLRDV
                     SGLQKKPSTSELIDWVQALARGGVDPGEIAARLPFMGVLLKKDHDVDVAQRALAGVRG
                     RR"
     gene            1048..2256
                     /locus_tag="VFZ86_13835"
     CDS             1048..2256
                     /locus_tag="VFZ86_13835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX5643397.1"
                     /translation="MFTDFFFLLRFYGVPVTVTEWLALMRGLAMGLVPAGLDEFYAVA
                     RSILVKSEAYFDQYDQAFAAMFKGIETPVQIEDEVWDWLSDPLTMPGLSHEERRLLAA
                     ALEDLDLEALQKLFEERLREQTEAHHGGSHWVGTGGTSPFGHSGYHPGGIRVGGESRN
                     MSAVKVAGERRYRGYRTDQEVGVRQFELALRRLRRLSSRNEGVPDELDLDETVEETAS
                     NAGRLSLVWRKSRKNAVKVLLLMDVGGSMHPYIRLCSQLFSAVDRSTHFKALRTYYFH
                     NCVYDWLYTSTRMTSSEAISTRRVLQELPSDYKLIVVGDAAMAPSELMARDGIIWWGF
                     GNDEPGIEWLRRLRRHFDHSVWLNTIPEADWDRAYGSVTIRKVREVFPMFELTVDGLT
                     AATRKLMVRY"
     gene            complement(2240..3949)
                     /locus_tag="VFZ86_13840"
     CDS             complement(2240..3949)
                     /locus_tag="VFZ86_13840"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX5643398.1"
                     /translation="MLYRRAIETSAGQDRGPQRRRSAERRRRLRRRRGVALLLLVVVL
                     ALVVWRAAACGGCGCGSGAEDGEQVAQSGPVPAPPAPELAGDGVKVGTFLGNYGRRYY
                     GQGPAPKRLDVLWKVRLGSGWSSGKFDTDPPSKWAGSGWTGQPSVVVDGGRAYVIASS
                     YAYKLRKIDAETGEVLWAYRYDDIIKGSPSVFRNPSPTGPDDKYIVVAGSRRGYPYEL
                     DDPRVAPVRAVTFGSGKELWRLPVPQTRCYSRDCDGSGFYYDGAYYVGVESGYFYALD
                     PLESQPWAEGDKPVIAAERLLLGDERAKNHGRNLVMESSVSALGENLYVASGAGHVYG
                     LRRSDLEVVWDYFIGSDLDGTPVTTSDGLLLQAVEKEYIKGKGGMLCLDPSRPAAEAT
                     VWYFPTGNRKVGDWAGGVVGSASVNDAYNPGGKYPALCAFSAIDGYLYVVARDVMSEE
                     TVAGPNREKGLKTPVEVARIWNGGAISTPILVGDALVAGSYDQRVHLWSVRYDPAEQG
                     DAGALPSASGDGTYWTVTIEERDQFYAEGAFESTPALWDGRIYIGCRDGWFYCLGDAS
                     TAP"
     gene            4185..5255
                     /locus_tag="VFZ86_13845"
     CDS             4185..5255
                     /locus_tag="VFZ86_13845"
                     /inference="COORDINATES: protein motif:HMM:NF017181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX5643399.1"
                     /translation="MTTDLDLLRDLCAAPAPTGFEAPVQDLVRRRLSALSAPEGDPLG
                     NVWATVDGKGAPHVVVTAHADQIGLIVTYVDEHGFVSFDKIGGVDPQLLPGRNLIVHG
                     AKGLVNGVVGRKPSHKMSKDERAKAPDLSDQWLDIGCSTRDEALELVQIGDPITFPPN
                     FLELAGARYASPAFDNRAGVYVCLRALEHYAEAPAAADLTALLTVHEETTFMGARAMA
                     IRWQPDVVIVVDVDFASDDPGLDAKKLGGEVKLGAGPVIHRGAAGNLALTDLAIQVAR
                     DEGIPVQVKAMPGGTSTDAEELMAAGAAATLSFSLPVRNMHSAFEVMQPDDAEAGALL
                     AAAVTRRIAADGWDAGRFVPRV"
     gene            complement(5252..>6189)
                     /locus_tag="VFZ86_13850"
     CDS             complement(5252..>6189)
                     /locus_tag="VFZ86_13850"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEX5643400.1"
                     /translation="GGAPQDPVSGPEAGMTRRQLLVGGGVVVAGLAVAGVVGWRSWTV
                     RDSWYRLTGAYGEPGTPPPAYEVTYERGTLPSTHLSEPAAYDIAYPAGVAGGSNQGGA
                     PYPVLICLPGRGRPPEALLEGGLRFGDFVADGIERRGVPPFAVAALQAGDTYWHARAS
                     GDDAMAVLFDEFIPFLRDQRGLTGPLAIMGWSMGGYGALRAAELHPQDFAAVCGVSAA
                     LWRSYEDGVGDAFDSAADYARNDVYADAGKLRGLPVRVDCGRQDPFYDADVAFTQALP
                     ESPAGGFEPGGHNDDYWSRVAPAEIDWIGAQFARR"
BASE COUNT          908 a         2325 c         2114 g          842 t
ORIGIN      
        1 ccaccgggtg ccgcctcggc gctcccacgc tcacgtcaac gaccgaaagc gcgaggccat
       61 gagccagtcc ttcacctaca cgggcaccag cgactacatc gccgcgcccg acctcatgca
      121 gacggtcaac gtcgccgtgc aactcagccg cccgctgctc atcaagggcg agcccggcac
      181 cggcaagacg atgctcgcgc agagcatcgc gcgcggcctg gacatgccgc tcctggtctg
      241 gaacgtcaag tcgacgacca aggcgcagga cggcctctac gtctacgaca ccgtgcagcg
      301 cctgtacgac ggccagttcg gcgaccgcga cgtgagcgac atcgcccggt acatcaagcc
      361 cggcaagctg ggcgaggcct tcgccagcga ggagcgcgtg gtgctcctca tcgacgagat
      421 cgacaaggcc gacctcgagt tccccaacga cctgctgtgg gagctcgacc agatggagtt
      481 ccacatcccc gagacgcacg cgaccgtcaa ggccgtgcac cggcccatcg tgctcatcac
      541 ctccaacgcg gagaaggaac tgcccgacgc gttcctgcgc cgctgcgtgt tccactacat
      601 cgagttcccg gacgaggaga tgatggagcg catcgtgcgc gtccacgtcc ccgacctgga
      661 caagcgcctg ctcgacctgg cgatccaggc cttctactgg ctgcgcgacg tgtccggcct
      721 gcagaagaag cccagcacca gcgagctcat cgactgggtg caggcgctgg cgcgcggcgg
      781 ggtcgacccc ggggagatcg ctgcgcgcct ccccttcatg ggcgtgctgc tcaagaagga
      841 ccacgacgtc gacgtcgcgc agcgggcgct cgcgggcgtc cgcggccggc gctagccatc
      901 cgggccgcgg cccacacgag cgaggagccc ccatgagcag cgtcggcggc atcgaactct
      961 tcatcgtcat cgtcatcgcg atcgtggtgg ccgggctggt gctgcgccag atcatgtcca
     1021 agcgctgagg cgcgccgaga gccgcgcgtg ttcaccgact tcttcttcct cctgcgcttc
     1081 tacggggtgc cggtcacggt cacggagtgg ctcgcgctca tgcgcgggct cgccatgggc
     1141 ctcgtgccgg ccggcctcga cgagttctac gccgtggcgc gcagcatcct cgtcaagagc
     1201 gaggcctact tcgaccagta cgaccaggcg ttcgcggcca tgttcaaggg catcgagacg
     1261 ccggtacaga tcgaggacga ggtctgggac tggctctccg acccgctcac gatgcccggg
     1321 ctcagccatg aggagcggcg cctgctggcc gccgcgctcg aggacctcga cctggaggcg
     1381 ctccagaagc tcttcgagga gcgcctcagg gagcagaccg aggcccacca cggcggcagc
     1441 cactgggtgg gcaccggcgg caccagcccg ttcggccact ccggctacca ccccggcggc
     1501 atccgcgtcg gcggcgagag ccgcaacatg agcgccgtca aggtggccgg agagcgccgc
     1561 taccgcggct accgcaccga ccaggaggtc ggcgtgcggc agttcgagct cgccctgcgg
     1621 cgcctgcgcc ggctgagctc gcgcaacgag ggtgtgccgg acgagctcga cctcgacgag
     1681 acggtcgagg agacggcgag caacgccggg cgcctcagcc tcgtctggcg caagagccgc
     1741 aagaacgccg tcaaggtgct gctgctcatg gatgtcggcg gctccatgca cccctacatc
     1801 cgcctctgca gccagctctt cagcgccgtg gatcgcagca cgcacttcaa ggccctgcgc
     1861 acctactact tccacaactg cgtctacgac tggctctaca cgagcacgcg catgacgtcg
     1921 tcggaggcga tctccacgcg gcgcgtgctg caggagctgc ccagcgacta caagctcatc
     1981 gtcgtcggcg acgcggccat ggcccccagc gagctcatgg cccgcgacgg catcatctgg
     2041 tggggcttcg gcaacgacga gcccggcatc gagtggctgc ggcgcctgcg ccgccacttc
     2101 gaccactccg tgtggctcaa caccatcccc gaggccgact gggaccgcgc ctacggcagc
     2161 gtgaccatcc gcaaggtccg ggaggtcttc cccatgttcg agctcaccgt ggacgggctc
     2221 accgccgcca cgcgcaagct catggtgcgg tactagcgtc ccccaggcag tagaaccacc
     2281 cgtcccggca gccgatgtag atgcgcccgt cccagagcgc cggcgtcgac tcgaacgcac
     2341 cctccgcgta gaactggtcg cgctcctcga tggtcaccgt ccagtaggtg ccgtcgccgc
     2401 tggcgctggg cagcgcgccg gcgtcgccct gctccgccgg gtcgtagcgc acgctccaga
     2461 ggtggacgcg ctggtcgtag cttccggcca ccagcgcgtc cccgaccagg atcggcgtcg
     2521 agatggcccc gccgttccag atcctggcca cctccaccgg agtcttcagt cccttctccc
     2581 ggttcggtcc cgccacggtc tcctcgctca tcacgtcccg tgcgaccacg tagaggtagc
     2641 cgtcgatcgc gctgaacgcg cagagcgccg ggtacttgcc tccggggttg taggcgtcgt
     2701 tgacgctcgc cgagccgacg acgccgccgg cccagtcacc gaccttgcgg ttacccgtgg
     2761 ggaagtacca caccgtcgcc tcggccgccg gccgcgacgg gtccaggcac agcatgccgc
     2821 ccttgccctt gatgtactcc ttctccacgg cctggagcag caggccgtcg ctggtggtca
     2881 cgggcgtccc gtccaggtcg gagccgatga agtagtccca gacgacctcg aggtcggagc
     2941 gccggaggcc gtacacgtgg ccggcgccgg acgcgacgta caggttctcg ccgagcgccg
     3001 agacggacga ctccatcacg aggttgcgcc cgtggttctt cgcgcgctcg tcgcccagca
     3061 gcagcctctc cgcggcgatc accggcttgt cgccctcggc ccacggctgg ctctcgaggg
     3121 ggtcgagggc gtagaagtac ccgctctcga ccccgacgta gtaggcgccg tcgtagtaga
     3181 acccgctgcc gtcgcagtcg cggctgtaac agcgggtctg cggcaccggc aggcgccaga
     3241 gctccttgcc gctcccgaac gtgacggcgc ggacgggcgc gacgcgcggg tcgtccagct
     3301 cgtaggggta cccgcgcctc gacccggcga cgacgatgta cttgtcgtcg gggcccgtcg
     3361 gcgaggggtt ccggaacacc gacgggctgc ccttgatgat gtcgtcgtac cggtaggccc
     3421 agaggacctc gccggtctct gcgtcgatct tgcgcagctt gtacgcgtag gagctggcga
     3481 tgacgtaggc cctgccgccg tccacgacga cgctgggctg cccggtccag ccgctgccgg
     3541 cccacttgct cggagggtcg gtgtcgaact tgccggagct ccagccgctg ccgagccgca
     3601 ccttccagag cacgtcgagg cgcttgggcg ccggtccctg cccgtaatac cggcgcccgt
     3661 agttgccgag gaaggtgccg accttgacgc cgtcgccggc gagctcgggc gccggcggcg
     3721 cgggtaccgg cccggactgc gcgacctgct ccccgtcctc cgcgccggag ccgcaaccgc
     3781 acccgccaca ggccgcggcg cgccagacga cgagcgcgag cacgacgacg agcaggagga
     3841 gggcgacgcc gcgccggcgc cgcagccggc gccggcgctc ggcggagcgg cggcgctgcg
     3901 gcccccggtc ttgtccggcc gacgtctcga tggcgcgacg atacaacaac cggccccgcc
     3961 gcgcccgacg cgcgccgcgg ggtcgcccgt gggggcgcgg cgtgtgcggt cgtggtgacg
     4021 tcaccggtgg cgtcgtcgtt ggcgtcactg gtgtcgcgtc gtgcgtgacg ccgcgcgcag
     4081 gcgcgctacc ttccagcggc gcgccgtacc gggtaggatg cctgaggccg gccggcgcag
     4141 ccgcgccggc cctcacgccg ccgaacgacc cccggagggt tcacatgacg accgatctcg
     4201 acctgctccg cgacctctgc gccgccccag ccccgaccgg cttcgaggcc cccgtccagg
     4261 acctcgtccg gcgccgcctc tccgcgctct cggcgcccga gggcgatccc ctgggcaacg
     4321 tgtgggccac ggtcgacggc aagggagcgc cgcacgtcgt cgtcaccgcg cacgccgacc
     4381 agatcgggct catcgtcacc tacgtggacg agcacgggtt cgtctcgttc gacaagatcg
     4441 gcggcgtcga cccgcagctc ctccccggcc gcaacctcat cgtccacggc gccaagggcc
     4501 tggtgaacgg cgtggtcggc cgcaagccct cgcacaagat gagcaaggac gagcgcgcca
     4561 aggccccgga cctgagcgac cagtggctcg acatcggctg ctccacccgc gacgaggccc
     4621 tcgagctggt gcagatcggc gaccccatca cgttcccgcc gaacttcctc gagctggccg
     4681 gcgcccgcta cgcctcgccg gccttcgaca accgcgccgg cgtctacgtc tgcctgcgcg
     4741 cgctcgagca ctacgcggag gcgccggcgg cggccgacct cacggcgctg ctcacggtgc
     4801 acgaggagac gaccttcatg ggcgcccggg cgatggccat ccgctggcag ccggacgtcg
     4861 tcatcgtcgt cgacgtggac ttcgccagcg acgaccccgg cctcgacgcc aagaagctcg
     4921 gcggcgaggt caagctcggc gccggcccgg tgatccaccg cggcgccgcc ggcaacctcg
     4981 cgctcaccga cctggccatc caggtcgcca gggacgaggg catcccggtg caggtcaagg
     5041 ccatgccagg cggcacgtcc acggacgccg aggaattgat ggcagccggc gccgcggcga
     5101 cgctgtcgtt cagtctcccg gtgcgcaaca tgcactcggc gttcgaggtc atgcagcccg
     5161 acgacgccga ggccggcgcg ctgctggcgg cggcggtgac gcggcgcatc gccgccgacg
     5221 gctgggacgc cggccgcttc gtgccgcgcg tctagcgcct cgcgaactgc gcgccgatcc
     5281 agtcgatctc ggcgggcgcg acgcggctcc agtagtcgtc gttgtggccg ccgggctcga
     5341 acccgcccgc cggcgactcc ggcaacgcct gcgtgaaggc cacgtcggcg tcgtagaacg
     5401 ggtcctgcct gccgcagtcc acccgcaccg gcaggccgcg cagcttgccg gcgtccgcgt
     5461 acacgtcgtt gcgagcgtag tcggcggcgc tgtcgaacgc gtcgccgacg ccgtcctcgt
     5521 aggagcgcca cagggcggcg ctcacgccgc agaccgcggc gaagtcctgc gggtgcagct
     5581 ccgccgcccg cagcgccccg tacccgccca tggaccagcc catgatcgcc agcgggccag
     5641 tcaggccccg ctggtcgcgc aggaacggga tgaactcgtc gaagagcacc gccatggcgt
     5701 cgtcgcctga cgcgcgcgcg tgccagtacg tgtcgccggc ctgcagcgcg gcgacggcga
     5761 acggcgggac gccgcgcctc tcgatgccgt cggccacgaa gtcgccgaag cgcaggccgc
     5821 cctcgagcag cgcctcgggc ggccggcccc ggccgggcag acagatcagg accgggtagg
     5881 gcgccccgcc ctggttcgac ccgccggcca caccggccgg gtaggcgatg tcgtaggcgg
     5941 ccggctcgga gaggtgcgtc gacggcagcg tgcccctctc gtaggtgacc tcgtaggccg
     6001 gcggcggcgt ccccggctcg ccgtacgcgc cggtgagacg gtaccaggag tcgcgcacgg
     6061 tccaggagcg ccagccgacg acgccggcca cggccaagcc ggcgaccacg acgccaccgc
     6121 cgacgaggag ctggcgacgg gtcatcccgg cctccgggcc ggagacaggg tcctgcggcg
     6181 cgcctccgg
//
LOCUS       DASUMX010000293         4865 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_112145, whole genome shotgun sequence.
ACCESSION   DASUMX010000293 DASUMX010000000
VERSION     DASUMX010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4865
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112145"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="VFZ86_13855"
     CDS             <1..255
                     /locus_tag="VFZ86_13855"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEX5643401.1"
                     /translation="PVLLGPAYDDLRRMETSVRQSELDWTIVRVSGALTDGAPTGKYR
                     AEPGYSLPGGRRLARADVAEYMLDQLTLKDDIGHAVAVAY"
     gene            complement(262..1629)
                     /locus_tag="VFZ86_13860"
     CDS             complement(262..1629)
                     /locus_tag="VFZ86_13860"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HEX5643402.1"
                     /translation="MPIFEWRGAMPASADEVFAYHARPGAFRRLAPPWQKLVVLEESG
                     DVTAGRVAFDVWFGPLKRRWLAEMGSAVPGRQFVDRQLEGPFASWEHVHRFVPIDERH
                     SEIIDHIEYSLPAGMLTDDVAEGPAGKTLSRLFRFRHERTRLDLGRHAEWADRPRFTV
                     AIAGASGLIGSNLADYLTTAGHRVIRLVRNSGGGPDEIPWDPSTGALYHGALEGVDAI
                     VNLSGANLAGIWTPAHKKAILESRVRTTQTLAEAITRMEHPPAVFVSGSAVGAYGSRG
                     AEVITEQTERGEGFLADVCRAWEEAAAPVADAGVRLVTPRFGLVVAAAGGAFAAMLPA
                     FKAGLGTRLGDGRQYWSWVALDDLLAALEWAIHDDEMEGVVNVTAPEPMTNAEVTQTL
                     GKVLRRPAALKAPAFLVRRATLGMGEQMLLASQRAMPAKLQEHGFRFAFPALEGALRH
                     ELGRS"
     gene            1789..2799
                     /locus_tag="VFZ86_13865"
     CDS             1789..2799
                     /locus_tag="VFZ86_13865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5643403.1"
                     /translation="MSRPEEADRSITAEALRRDFGELHAVDGVDLEIPGGQIFGFLGP
                     NGAGKSTLVKILTTILNATSGRATVAGFDVARQGGRVREAIGVALQDVGLDPLMTARE
                     LLMLQAELFGTSRDEARKTAERLLVTVGLDDVDPKKRAGAYSGGMKRRLDLALALVHD
                     PRVLFLDEPTTGLDPASRAAIWEEVRRLNDDLGMTIFLTTQYLEEADRLADQVAIINR
                     GKIVAQGTPEELKRQVGDEVVELQFDSSADAERAHDALAPVAPNRQRSNRQLRLYFGR
                     AAENVPELVRTLDGAGIRLQGLTIEQPSLDDVFLRVTGEALEHEIGEGAEDVEADAEV
                     RS"
     gene            2796..3557
                     /locus_tag="VFZ86_13870"
     CDS             2796..3557
                     /locus_tag="VFZ86_13870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014642977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5643404.1"
                     /translation="MNDTLLLIGRSLRNTMREPLTVLPNIAISVFFLFVYNSGLSSVA
                     ELPGFQGTYLGFILPVAIVSAAVGGAGLAGQTLIKDIDNGYFTKLLLTPTRRLSVIWG
                     PMIASAIVLTAQVVLVMLIALALGLKVAAGPAGFALVVVLAFLWGMAFAGYAAFMALK
                     TKNAAAAQAATFAFFPLIFLSYTFVPKEYVTAGWLKVAATINPTTYVFESMRSLLNTG
                     WDGTALAVGFAVTFGFATLTGIMALWAARGATKLS"
     gene            3568..>4865
                     /locus_tag="VFZ86_13875"
     CDS             3568..>4865
                     /locus_tag="VFZ86_13875"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5643405.1"
                     /translation="MSPPRLIPARLREGAGLLRSRDFRNVFIAQSVSVFGDGITPVVL
                     TFAVLDLTGSGTDLGIVLACQSIPLVLLALVGGVWADRLPRGAIMVASDLVRAAVQLT
                     AAALLLTGMAQIWQLAVLAACHGAAEAFFRPAAGAILPQIVPVRSLQQANALMGMSDN
                     FGWMVGPAVAGTLVALIGAGGAIAVDGATFLVSAAFLATLRVPAIVKSEAARGFVAEL
                     RKGWQEVRSRTWLWVMLLRVCLVLFIVIAPFQILGPLGLRDQGYGAAAWGWLQAVFSA
                     GMIVGMLIAMRYRPKRPMITVSLAGSTAITAPLAMALFGDPWILGVVYSLRGVSVGIL
                     VAVWNTTLQTQIPRESLGRVTAWDWMSSLALWPLGLAVAGPLAERFGVTTMCWVTAGL
                     GVVASFWVLFVKDVWRLRPAAVLAGSSGPSPPPAAAPPKD"
BASE COUNT          677 a         1758 c         1698 g          732 t
ORIGIN      
        1 ccggtcctgc tcggtcccgc ctacgacgac ctgcggcgca tggagaccag cgtgcgccag
       61 agcgagctcg actggacgat cgtgcgcgtc tcgggcgcgc tcaccgacgg cgcgccgacg
      121 ggcaagtacc gcgccgagcc cggctattca ctccccggcg gccggcgcct cgcgcgggcc
      181 gacgtggccg aatacatgct cgaccagctc acgctgaagg acgacatcgg ccacgccgtc
      241 gcggtggcgt actgagcccg gtcaggaccg cccgagctcg tgacgcagtg cgccctcgag
      301 cgccgggaac gcgaagcgga acccgtgctc ctgcagcttc gccggcatcg cccgctggct
      361 ggcgaggagc atctgctcgc ccatgcccag ggtcgcgcga cggacgagga aggcgggcgc
      421 cttcagcgcg gccggccggc gcaggacctt gccgagcgtc tgcgtgacct ccgcgttcgt
      481 catcggctcc ggcgcggtca cgttgacgac gccctccatc tcgtcgtcgt ggatcgccca
      541 ctccagcgcc gcgagcagat cgtcgagggc gacccaggac cagtactggc ggccgtcgcc
      601 cagccgggtg ccgagaccgg ccttgaacgc cggcagcatc gccgcgaagg cgccgcccgc
      661 cgcggcgacc acgaggccga agcgcggcgt gacgaggcgg accccggcat ccgcgaccgg
      721 tgcggccgcc tcctcccagg cacggcagac gtcggcgagg aagccctcac cccgctccgt
      781 ctgctctgtg atcacctcgg cgccgcgcga accgtaggcg cccaccgcgg acccgctcac
      841 gaagaccgcc ggggggtgct ccatgcgcgt gatcgcctcg gccagcgtct gcgtcgtccg
      901 cacgcggctc tcgaggatcg ccttcttgtg ggcgggcgtc cagatgccgg cgagattcgc
      961 gccggacagg ttgacgatcg cgtcgacgcc ttcgagcgct ccgtggtaca gagcgccggt
     1021 ggacgggtcc caggggatct cgtccggccc gcccccggag ttgcggacca gcctgatcac
     1081 gcggtgcccg gcagtggtca ggtagtcggc gagattggag ccgatcaacc cgctcgcgcc
     1141 ggcgatcgcc acggtgaacc gcggacggtc ggcccactcc gcgtgccgcc cgaggtccag
     1201 ccgcgtgcgc tcgtggcgga agcggaagag acgcgagagg gtcttgccgg caggcccctc
     1261 cgcgacgtcg tcggtgagca tgccggccgg caggctgtac tcgatgtggt cgatgatctc
     1321 gctgtgccgc tcatcgatag gcacgaaccg gtgcacgtgc tcccacgacg cgaatgggcc
     1381 ctccagctgg cggtccacga actgccgtcc cggcacggcg ctgcccatct cggccagcca
     1441 gcgccgcttg agggggccga accagacgtc gaaggcgacg cggccggcgg tgacgtcccc
     1501 gctctcctcc agcaccacga gcttctgcca cggcggcgcc agccgccgga aggcgccggg
     1561 tcgagcgtgg tacgcgaaga cctcatcggc ggacgccggc atggcgccac gccactcgaa
     1621 gatcggcatc gaacccctcc ttggtcgctg agccggtctt acccgggctg ggccgccgta
     1681 agcccgccga gaccccggtc cacgtggcct cgctccgagc cgcgattagc aggtggacgg
     1741 ctcgggtagt cccgaccgga gcacacgtgc ggcgacgggg aggagccagt gtccagacca
     1801 gaggaagcag accgcagcat cacggcggag gcgctgcgac gcgacttcgg tgagctccac
     1861 gccgtggacg gcgtcgacct ggagatcccg ggcggccaga tcttcggctt cctcggcccc
     1921 aacggcgccg gcaagtcgac gctggtgaag atcctgacca ccatcctgaa cgcgacgagc
     1981 ggtcgcgcga cggtcgccgg cttcgacgtc gcgcggcagg gcggcagggt gcgcgaggcg
     2041 atcggcgtcg ccctccagga cgtcggcctg gacccgctga tgacggcccg ggagctgctc
     2101 atgctgcagg ccgagctctt cggcacttcc cgagacgagg cacgcaagac cgcggaacgc
     2161 ctgctcgtga cggtcggcct cgacgacgtc gacccgaaga agcgcgccgg cgcgtactcc
     2221 ggcggcatga agcgccggct ggacctggcc ctcgcgctgg tgcacgaccc acgggtcctc
     2281 ttcctcgacg aaccgacgac cggcctcgat ccggccagcc gggcggcgat ctgggaggag
     2341 gtgcggcgcc tcaacgacga cctcggcatg acgatcttcc tgacgacgca gtatctcgag
     2401 gaggccgacc gcctggccga tcaggtcgcc atcatcaacc ggggcaagat cgtcgcgcag
     2461 ggcaccccag aggagctgaa gcgccaggtg ggggacgagg tcgtcgagct gcagttcgac
     2521 tcctccgccg acgccgagcg cgctcacgac gcactggcgc cggtggcgcc caatcgccaa
     2581 cgatccaacc gacagctgcg gctctacttc gggcgcgccg ccgagaacgt gcccgagctg
     2641 gtacgcaccc tcgacggcgc cggcatccgg ctgcaggggc tgacgatcga gcagccgagc
     2701 ctcgacgacg tcttcttgcg cgtcaccggc gaggccctgg aacacgagat cggcgaaggc
     2761 gccgaggacg tcgaggccga cgcggaggtg cgatcatgaa cgacaccctg ctcctcatcg
     2821 gccgctcgct gcgcaacacc atgcgtgaac cgttgacggt ccttcccaac atcgccatca
     2881 gtgtcttctt cctcttcgtc tacaacagtg gcctgtcgtc ggtcgccgaa ctaccggggt
     2941 tccagggcac gtatctgggc ttcatcctcc cggtcgccat cgtctcggcg gcggtcggcg
     3001 gcgccggcct cgccgggcag acgctgatca aggacatcga caacggctac ttcaccaagc
     3061 tgctcctgac gcccacccgg aggctctcgg tgatctgggg accgatgatc gcgagcgcga
     3121 tcgtgctcac ggcccaggtc gtgctcgtga tgctcatcgc cctggcgctg gggctcaagg
     3181 tcgcggccgg acccgcaggg ttcgcgctcg ttgtggtgct cgccttcctc tgggggatgg
     3241 cgttcgccgg gtacgcggcc ttcatggcgc tgaagaccaa gaacgcggca gcggcccaag
     3301 ccgcgacctt cgccttcttc ccgctcatct tcctgagcta caccttcgtg cccaaggagt
     3361 acgtcaccgc cggctggctc aaggtcgcgg cgacgatcaa cccgacgacc tatgtgttcg
     3421 agtcgatgcg ctcgctgctg aacaccggct gggacggcac ggcccttgcc gtcggcttcg
     3481 ccgtgacctt cgggttcgcg acgctgacgg ggatcatggc gctctgggcg gcacggggag
     3541 ccaccaagct gtcgtagact cgaggggatg tcccctccgc gtctcatccc tgcgcgcctg
     3601 cgcgagggcg caggcctgct gcgctcgcgc gacttccgca acgtgttcat cgcccagagc
     3661 gtgtccgtgt tcggcgacgg catcaccccg gtggtgctga cgttcgccgt gctcgacctg
     3721 acaggctcgg gcacggacct cggcatcgtg ctcgcctgcc agagcatccc gctcgtcctg
     3781 ctggcgctgg tcggcggcgt gtgggcggac aggctgcctc ggggcgcgat catggtcgcg
     3841 agcgacctcg tgcgcgccgc ggtacagctc acggccgccg ccctgctcct caccggcatg
     3901 gcgcagatct ggcagctcgc cgtgctggcc gcctgtcacg gcgccgccga ggcgttcttc
     3961 agaccggccg ccggcgccat cctgccgcag atcgtgccgg tccgcagtct ccagcaggcc
     4021 aacgcgctga tgggcatgag cgacaacttc ggctggatgg tcgggccggc ggtcgccggc
     4081 acgctcgtgg cgctcatcgg cgccggcggc gccatcgccg tggacggcgc gacattcctc
     4141 gtcagcgccg ccttcctcgc cacgctgcgg gtgccggcga tcgtcaagag cgaagccgcg
     4201 cgcggcttcg tcgccgagct gcgcaaaggc tggcaggagg tcaggtcgcg tacctggctc
     4261 tgggtgatgc tgctgcgcgt gtgcctcgtc ctcttcatcg tcatcgcgcc ctttcagatc
     4321 ctcgggccgc tcgggctgcg ggaccagggc tacggcgcgg ctgcctgggg ctggctgcag
     4381 gccgtgttca gcgccgggat gatcgtcggc atgctcatcg cgatgcggta ccggcccaag
     4441 cggcccatga tcaccgtgtc actcgccggg agcaccgcca tcacggcgcc gctcgcgatg
     4501 gccctgttcg gcgacccgtg gatcctgggt gtggtctaca gcctgcgcgg cgtcagcgtc
     4561 ggcatcctcg tggccgtgtg gaacacgacg ctgcagacgc agatccccag ggagtcgctc
     4621 ggccgggtga cggcgtggga ctggatgtcg tcgctcgcgc tctggccgct cggcctggcc
     4681 gtcgccgggc cgctcgccga gcggttcggc gtgaccacca tgtgctgggt cacggcgggg
     4741 ctgggcgtgg tcgcgtcctt ctgggtgctc tttgtgaagg acgtgtggcg gctgcggccg
     4801 gcggcggtgc tggccggctc cagcggccct tcgccgccgc cggccgcagc cccacccaag
     4861 gactg
//
LOCUS       DASUMX010000294        24282 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1981865, whole genome shotgun sequence.
ACCESSION   DASUMX010000294 DASUMX010000000
VERSION     DASUMX010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 24282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24282
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1981865"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..650
                     /locus_tag="VFZ86_13880"
     CDS             <1..650
                     /locus_tag="VFZ86_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5643406.1"
                     /translation="GAGKTTLFNAITGVYPPTSGAIRFKGRDIAGLLPSKVTKLGIAR
                     TFQNIRLFNEMTALENVMVGQHCRSHTNMLRVIARTPGAKRTEKAVRDRSLELVDYVG
                     LSGHGGELAKNLPYGSQRRLEIARALATDPTLLFLDEPAAGMNPQETAALMGLIRRIR
                     DEGMTIILIEHDMKVVMGSSDRVVVLDHGEKIAEGPPEQVRNDPKVIEAYLGKGA"
     gene            650..1396
                     /locus_tag="VFZ86_13885"
     CDS             650..1396
                     /locus_tag="VFZ86_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019433781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5643407.1"
                     /translation="MGAAAATAEKDGGALLAVRDIHVFYGNIEALKGVSLEINEGEIV
                     TLIGANGAGKTTTLNAISGLLRPRSGSIRYGGRDLAGVPAYEVVTQGLSQSPEGRKIF
                     PRMSVRENLEMGAFSRRGQDLKADFERVFDLFPVLRERESQPGGTLSGGEQQMLAMGR
                     ALMAGPKLLMLDEPSMGLAPMLVEKIFDIVKEINAQGTTILLVEQNAHVALEISSRGY
                     VLETGRVTLSDDASALLKNPHVREAYLGAD"
     gene            complement(1451..2449)
                     /locus_tag="VFZ86_13890"
     CDS             complement(1451..2449)
                     /locus_tag="VFZ86_13890"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HEX5643408.1"
                     /translation="MGQKSRRDRGRMSRIAVAALVVAAAIVILAAVTPREALAQLAYI
                     HGGIAVEDCETCHVNAHTWWTPTNEHCLGCHPGFQVPDTDLTCWTCHAPGQDMSASRV
                     DAACTAACHLRDGSTVTHQAHAGASDACTSCHPVSASPSEAAGSAHHTVPPPVVAAMT
                     PAFGLPGAQVTLAGSRFTGTTAVSFNGVLAVFAVDSDTRITAVVPPGATSGPVSVISR
                     GGKGAGPGAFTVTVVSSLTLRAGPAIQTLGRRVRLGGTLQPLGRAGTTLKVFVQQRRG
                     GAWRTVRAVSRDTSTAGVFAWSYAPPRRGAYRAAASLAASPASTAARSRWVTFRIR"
     gene            complement(2573..4807)
                     /locus_tag="VFZ86_13895"
     CDS             complement(2573..4807)
                     /locus_tag="VFZ86_13895"
                     /inference="COORDINATES: protein motif:HMM:NF014398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HEX5643409.1"
                     /translation="MGKKVSILTAVAIACMLLLPALASAQLVNEYGMHYAGQTKCLTC
                     HGGIMGPSELALHGRFAQSGILPDVPEGWTAFQGPGAVEPVAGEGQSMYHAGGSYSIT
                     DLDWITLGDSSEVGNSATEYLFFRGTEDPTVMPWNIVEGLSWEPAHGGEWMVAAGDPG
                     TGLFDESYACQRCHMMGSTVPGTGTVPNPAATIAPSPGTARQWARDESTTVQDFMDYA
                     SVSTPGLGIQCEQCHGTGEASATGHFTSGVVVSAGIDVLGKSQVCGQCHGSYTSVDGT
                     MGIYGYTTNLQLRDFVDINGVSGGQSYTKIPTEEEFAASPAAYWMFPNGSNAKGNHYY
                     YNEWAASGHSYRSALTKDDPDAMAFQAAGNGHYSNNFDPNLTGGCYKCHTGEGYLQSK
                     GSSLAADLVLTKDNVGQMGQECSTCHGGHPSGVGAADVVRDPDKAGERSAAGLSVDNA
                     SICEDCHNWQVEVTGQKPSPAPMADLAAHGSPSHPQRETLHGRSAMYDTAAGTEFMPG
                     AACEDCHMPKTNKAATRISHGMKPMLPGDAERWNGMAGAAYQGEDSCTGCHPSQSRSA
                     LQAEIDEWQEAAEAKAAKVAAAIEKAKTKSEFSATNSAKPGYVLVGRATWNYKVFEND
                     ASEGVHNPPYIMAGLVKAEQMAKSVGGRFMHAGAKSPVDKGANAHVVGRIVNGDNSAA
                     AGAKVQLMRGSKLLRTSLSDASGNFAFTFKVSKTYKNYRVRWIRSSASSTYKYSAYMT
                     IRIR"
     gene            complement(5279..6598)
                     /locus_tag="VFZ86_13900"
     CDS             complement(5279..6598)
                     /locus_tag="VFZ86_13900"
                     /inference="COORDINATES: protein motif:HMM:TIGR01910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HEX5643410.1"
                     /translation="MSQTLRSAFEERVVAAVREGEPELVALASDLVAFDTTARNLGDP
                     PRQEAELQEHLRRRLAALGAEVEVWEPEPTGTGNRVVPDGLDFRGRPQLAARLRGAGG
                     GRSLLLNGHIDAVSAEPLDRWTSHPHRPEVRDGQLYGRGSCDMKGGIAGMLFALETVT
                     RLGARLAGDVVFCTDTDEESSGAGAYACAAHGVRADAGLCAEPTAFDVWVACRGGVNP
                     VVRTVGRTGHAEMHHPHWRDGGPVNAIEKMEVVLASLHELRADWARRPEHQHKYLHVP
                     DVLPTVIRGGEWMVTYPSSCEAVLSVQYMPGRVDGDRGGQDVFDEVEAWVKAAADRDD
                     WLAEHPPEWEWPCDIVPAEVPDDAPIVTGTLAAAADVGRPGRISGLDSWHDAAVFTRV
                     AGTPTVSFGPGDLARAHTIDESVPVADLVDHAAAVALMLLRWCGSAG"
     gene            complement(6711..8300)
                     /locus_tag="VFZ86_13905"
     CDS             complement(6711..8300)
                     /locus_tag="VFZ86_13905"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX5643411.1"
                     /translation="MAEDLVFARKASGLVRGLDMWDAFGAGLMTCQPIATLWLMVGIA
                     LSLFPTGNLLLAIALGAVTAGIGAPLVWGILSGSMPRAGGEYVYNSRILHPAIALGAV
                     FANILAIFYWNWFIATWLGVPALQLFGQYMGWTGFADWAASKTGLFVLGLIAVVIGYL
                     SVAFGMKIYSRIQKILLIPAIGGPIVLIIALFLSSKAEFIGNWNAIAAEFGSLDYEAF
                     IQAAGPLPTTWDWHGTLGALSGVYALFVYNYAIAYLSGEVKRPDKSLMGGNILAVWVP
                     IVLGVLTVLGLYRVAAFNFISATAQASFGAGVEGYTAPYSPDYLTLSWIASGQSGWVA
                     WAILLGFIAVIWLEITIAILIIGRAFLAWGLDRMGPKWFTDVSPQYAQPIKNLTFALV
                     VFAVGVAVYVLWLQSDLSGLIGGGMQFISVFMVTGISALLFPYRKNVRGIWEASPYRT
                     WKIAGIPLVSIGAVLYLIFIFAFLYFSFIDPTSRDVTGKNLFVFAAAWVAGLAWYFFW
                     RARSKKQGIDVSITFGELPPE"
     gene            complement(8472..8987)
                     /locus_tag="VFZ86_13910"
     CDS             complement(8472..8987)
                     /locus_tag="VFZ86_13910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEX5643412.1"
                     /translation="MNAIVVYESLWGNTAEVARAVAEGLGPGARALRTDEASAADIAG
                     ADLVVAGAPVFGFKLSSQKMRDGIRRSPGKGPAPDLSCPLLRAWLAELPHGSAGCAAF
                     DTQVRGPFGKGAPEILELLEARGYARVAEPQGFVVKGSQGPLRKGEAERARAWGAELA
                     RSSGAKRSLTP"
     gene            9133..11631
                     /locus_tag="VFZ86_13915"
     CDS             9133..11631
                     /locus_tag="VFZ86_13915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5643413.1"
                     /translation="MAAEERLRDATVTATEFDLRAAEAPPARARAVVVGGGIIGASIA
                     YHLARLGWTDTVVLERARVSSGTTWHAAGLMTRTRGTHVQTELASYSRDFYEGLEALS
                     GVDVGYYPNGSLSVARTPERLTELRYALSLARHHGLPARELTAEGIAGVSPLLVVGDL
                     VGGVLFEEDATVNPGWAAYATAKAAFDLGARLVEDVQVTGFRLRGGRVVAVETDRGAV
                     ECEIAVIAAGLWSRDLGLLAGVRLALHPSEHYWAQTEPVDGATRDLPIIRDLDGSIYV
                     RHYRGGLLVGAFEPDGRPRTTASIPPDFAFGEFEPDLEHFARPLAQATVRVPALGQAR
                     FAHYLCAPESFTPDGNFLLGETAEVAGLYVAAGMNSQGIILGPGAGRALAEWIEAGAP
                     TVDVADLDVRRFSPAQGNASYLFSRTRESLGRLYAMHWPFLQPGTARGLRRVPLYDRL
                     AAAGACFGETAGWERPNWYAPPGERPEYVYSYGRQNWFGAVAGEHRAAREGVALFDLS
                     SFAKIRVEGPEALPTVQRVFTADLDRPPGSVVYTAMLNARGGVELDATVTRLAGDAFL
                     VVTSAAAQHKAFQWLRRNAGAGAVVTDVTSGLGVLSVTGPRARDLLAGLTEADLSPAA
                     FPFARAREIDVGWATVLALRVSFTGELGWELYAPVESLPVLYDQIAGAGAEHGLRHAG
                     YHALDGLRAEKGFVHWGADAGPADTPPETGLEAVVAPDKPGGFIGREALQAAGPPRRR
                     LVPILLADPEPLLYHGESVLQGGRVVGRVTSGAYGHTLGAAVGLAAIAGEPGQIDAIV
                     AGGAVEVEIAGERAPARLSARAFYDPDGLRLRGS"
     gene            11680..12849
                     /locus_tag="VFZ86_13920"
     CDS             11680..12849
                     /locus_tag="VFZ86_13920"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX5643414.1"
                     /translation="MLPQKWATTRAGLAPALGIAGALCLLVAGVIGIASAQGGPAVRA
                     SSSPSPSPSASASPGVSETLTTAVVRAVTVHRGSVAGVRYRADDTAGGTVTLDLLITT
                     RAGAVVRRLATGRVTEVGVERVWRGRLRLRRGRYLLVAHAVDASGRSEAHARTAELRV
                     LAPLPPLVPTASARRAAFAWAARRAGDVAVAVVDSRGRAYGYREQRSFITASVVKAML
                     LVAYLRRHDTVDPVMRATLTRMITVSDNAAADAVYRSVGREGLARFAGRAGMRSFRAS
                     GAWILCRVAAADMARFFRDMERYIPSRHRSFANGLLAGVVSYQRWGIPAAAGPLGYRV
                     YFKPGWLGAWVLASQAARLERRTVRIGLAVFTDRNPSSTYGKETVAGVTARLLRR"
     gene            complement(12859..13899)
                     /locus_tag="VFZ86_13925"
     CDS             complement(12859..13899)
                     /locus_tag="VFZ86_13925"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006104208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008720 - D-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0070404 - NADH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HEX5643415.1"
                     /translation="MRLSVFSSKPHDRQFLARVNDEEGFGHELVFFDARLEPATARLA
                     HGSSAVCAFVNDDLGEQVLADLVAHGTRLLALRSAGFNNVDLGAAHDLGLTVMHVPDY
                     SPYAVAEFTIALVLALNRHLSRAYTHVREGNFSLQGLLGFDLHGKTAGIVGTGKIGAI
                     VAETLLRGFGCRVLAHDLSPSDRLRRMGVEYLGLDALLSASDLVTLHCPLTAETHHLI
                     DRRSIAGMKPGVMLINTSRGGLIDTGAVIEGLKSGRIGHLGLDVYEEEALYFYEDYSS
                     RLIDDDALSRLLTFPNVIVTSHQGFFTREALEQIARTTLANVRDFEEGGESPNEVRSR
                     RDERNGRRGMAA"
     gene            14047..14307
                     /locus_tag="VFZ86_13930"
     CDS             14047..14307
                     /locus_tag="VFZ86_13930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643416.1"
                     /translation="MSAKRLAYSRYQILCAERDAWLEAETGDTDAYYYDGISFWRVAG
                     DGRKRLVPSWRAPAHGWRHDEDCTCDLCTAYRPARLRPASVA"
     gene            complement(14320..15243)
                     /locus_tag="VFZ86_13935"
     CDS             complement(14320..15243)
                     /locus_tag="VFZ86_13935"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020178659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEX5643417.1"
                     /translation="MRIVVHGQQAFGKAVLEALLGRGDEVVAVYASPAGKREDPLAEA
                     ALAAGLPLVRPASFRDPAVTEEFRAFAPDLQVMAFVTLIVPAEFLAVPAYGTIQYHPS
                     LLPRHRGRSAINWPIIKGETETGLTVFCPDGGIDTGEILLQKHTPIAAGDTLGSVYFE
                     RLFPMGVEALLEAVDLVNAGTAPRLPQDESLASYEAPCERENARLDWGKPCRQLHDLV
                     RGCDPAPGAWTTSGGQALQVVAATPRPARDVAGVGGAMGEVVDVGEDGFSVVCADGRL
                     RVTRVRPEGGVKTGAGEWAAASGLRPGARFV"
     gene            complement(15507..16598)
                     /locus_tag="VFZ86_13940"
     CDS             complement(15507..16598)
                     /locus_tag="VFZ86_13940"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HEX5643418.1"
                     /translation="MRKATFLGLVSLLTLLVFATTAVAGPIKDKKARLAAVQAQLSKV
                     YEQSDMAVERYNEAASRLGDVQDRITRNQHLLKVAEYKLGVANDHLTSRARQVYKADD
                     AGILDVVFASRTFEDLLTQIDMMQRMTESDARTARAARAYRQEISDRRVALEEDRKSA
                     VRLVAQREESKDQVLALQAQLESTAGGLKDEIAELQAKAAAAAKKAAERAAALAASQA
                     SSSAASSSSGSSSGGSSSSGSGGGTVIDPGGSGRSAVVAIAQRYLGVPYVWGGASPSG
                     FDCSGLTMYCYAQIGIGMSHGATDQQRQSKPVSLSALQPGDLVFFGSASYSHHVGIYA
                     GGGSMIHAPHTGAVVSYGSISGAWIGGRF"
     gene            complement(16766..17164)
                     /locus_tag="VFZ86_13945"
     CDS             complement(16766..17164)
                     /locus_tag="VFZ86_13945"
                     /inference="COORDINATES: protein motif:HMM:NF016363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEX5643419.1"
                     /translation="MASARDPHDHWHDDHDTGDDAGTGWDRWPKPRPLELVSHPQPAF
                     ELVKPESFEAAQTIADSVRAGIPVLVDFHDCDPGLAGRLTDFCSGLAYAVEGALQHVG
                     RDVLLIAAHSVDVHGDVASAVRSPGFYNRS"
     gene            17372..18859
                     /locus_tag="VFZ86_13950"
     CDS             17372..18859
                     /locus_tag="VFZ86_13950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HEX5643420.1"
                     /translation="MPDLDLVLVLACALVLLSILVSKASLRTGIPALLVFLAIGMLAG
                     SEGLLGIYFDDAWLARAVGVVALALILYAGGLDTRWESIRPVLGRGVLLATFGTLLTA
                     GLVGMLVLWLTDLPVMQALLLGAVVSSTDAAAVFSVLRARQVRLPGDVRSILELESGT
                     NDPMAVFLTVSLIALITTGGGSPLDLAVSFVLQMGIGLLFGYALGRAMAWALDRINLE
                     HDGLYPVMSLALVLLAFGATQYVGGSGFVAVYVAGLVMSGRVYLHKRSLARFHDGLAW
                     LAQITMFLTLGLLVFPSRILPVLGVAVLVTAFLTLIARPLAVYVSLIRSRFTMRERTL
                     IGWVGLRGAAPIVLATFPLVAGISGGDTLFNIVFVVVLASTLIQGTSIPMVARWLGIE
                     GPAFESEPAWAGEPVRRELVEFRVPETSPLVGRRVAQAGLPAGADAVLIHRYDSYLVV
                     RGSTRLRRGDVVLLLADEPALRELEARRELERRPTLAACETDLTA"
     gene            complement(18915..20033)
                     /locus_tag="VFZ86_13955"
     CDS             complement(18915..20033)
                     /locus_tag="VFZ86_13955"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX5643421.1"
                     /translation="MTTVEGPRKVYVEPTNACNLDCRTCVRHSWDEPEGFMEWATFAA
                     VVDGLAPAPASEAGGVAFMGLGEPLLHPRFLDMVRKVKESGLRAEVTTNALLLDDEMA
                     AGLLQAGLDQLVVSIDGASAEAFGRVRSGASLERVVENVRRLHDAKGPNYGPSLRIGV
                     EFVAMRSNVEELPGLGRLAAQLGATFIIVSNVLAYTEDLLAETLYDHHASSLSGAETT
                     AAPRWQLPAFDWDRQLGAALGEALTRSGPVSFFGAETEATRSHCPFVEADACAVAWHG
                     GVSPCPPLLHTYTCFVRGREKRMLRWEAGRLPDETLAGIWEKPEYVAFRDRVRRFAFP
                     PCTDCGCELAEANEEDCFGNPHPACGDCLWARGVIRCA"
     gene            complement(20044..20274)
                     /locus_tag="VFZ86_13960"
     CDS             complement(20044..20274)
                     /locus_tag="VFZ86_13960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HEX5643422.1"
                     /translation="MTAFVWGPTLDEAICTDCRVCLEFCRQGVYAMVDGHVRVVAKAS
                     CMEGCSHCAGMCEAGALTFPTLDALRAAKRKA"
     gene            complement(20288..20908)
                     /locus_tag="VFZ86_13965"
     CDS             complement(20288..20908)
                     /locus_tag="VFZ86_13965"
                     /inference="COORDINATES: protein motif:HMM:NF022321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2703 domain-containing protein"
                     /protein_id="HEX5643423.1"
                     /translation="MSDPQTTARPTSSCCGDQSAASPCCGGAPTAQAAAGPPADATPP
                     RRLNIELLVIDLESCARCVPTGEQLRNAIELVAPAAEAQGIGLTYRETVVQTAEEAKA
                     RALLSSPTIRINGRDIDQDIRESECESCGDLTDNDVSVDCREWHYRGQVYSAAPLPLL
                     VEKLMDAMLKLDESPPITPEPLAELPENLQRYFANKRPAAGSGCCC"
     gene            complement(20969..22180)
                     /locus_tag="VFZ86_13970"
     CDS             complement(20969..22180)
                     /locus_tag="VFZ86_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020881611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEX5643424.1"
                     /translation="MTAEALEEPVAAAAGQAVVEGPDPDDAPARVVPACECAAPPATA
                     SEPPDADAVAGRRAPFRAGARALGRLPWWARVAGGVALWALVYHYWLPFIVWLFHDVL
                     SLPDTRWASALEFFVYDSVKVMLLLTIVVFGVGIVRSFFSPQRTRSLLRGRRETTGNV
                     MAAGLGIVTPFCSCSAVPLFIGFVEAGIPLGVTFSFLIAAPMVNEIALVLLYGLFGWE
                     VAALYLVTGLLIAIVAGWVIGRLRMDRYVEDWVREIRMGDTAGAQELDWPGRVSYAWQ
                     AVRDIVGKVWPYVLAGIAVGAVIHGYVPEDFMASIMGADAWWSVPLAVLFGIPMYSNA
                     AGIIPIVEALLEKGAALGTVLAFMMAVIALSLPETIILKKVLKWPLIFAFLGVVGTGI
                     MFVGFLFNAVL"
     gene            complement(22177..22926)
                     /locus_tag="VFZ86_13975"
     CDS             complement(22177..22926)
                     /locus_tag="VFZ86_13975"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX5643425.1"
                     /translation="MRTLRIATPFAVFSDVHGNLPGLEAILADVDRRKVSATISLGDL
                     VGYGPSPNEVALLVRERGIPSLMGNYDQGIAFETGDCGCVYKTDQQRAEGAASLAWTQ
                     QAVTEEVKAYLRMLDDRFELDTPAGTLLAVHGSPRRINEYLFEDRPEKAMARMADEFP
                     YPAILFGHTHVPYARPVGETVFVNVGSGGRPKDGDWRVCYAIVDPTKLGSEGFVEFVR
                     VPYDHERLRLELAATPLITSFAGPERGGVET"
     gene            23002..24129
                     /gene="arsB"
                     /locus_tag="VFZ86_13980"
     CDS             23002..24129
                     /gene="arsB"
                     /locus_tag="VFZ86_13980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HEX5643426.1"
                     /translation="MAQSTVKRLSFLDRYLTLWIFLAMFIGVGIGWAFVSVPEFIDRW
                     TVGTTNIPIAIGLIVMMYPPLAKVRYEELGDVFRDYRILGLSLVQNWLIGPFVMFFLA
                     IIFLHDYPEYMVGLIMMGLARCIAMVIVWNQLAKGDTEYAAGLVAFNSVFQVLFYSVF
                     AWVFVTKLPPLFGLEGVAVDITIGQIAKSVFIYLGIPFLAGMLTRFVALKTKGREWYE
                     QKFIPRISPLTLIALLFTIFVMFSLKGDLIVELPLDVLLIAIPLSLYFVIMFFVSFFM
                     GRKIGADYSKTATLSFTAASNNFELAIAVSIAVFGLNSGQAFASVIGPLVEVPVLISL
                     VSVALWLGRKYWGMTDEPVPAAVAAGCDEVGCDPVRGRETS"
BASE COUNT         3403 a         8616 c         8682 g         3581 t
ORIGIN      
        1 acggagccgg caagaccacg ctcttcaacg ccatcaccgg cgtctacccg cccacgtccg
       61 gagccatccg cttcaagggt cgggacatcg ccggcctgct cccctccaag gtcaccaaac
      121 tcggcatcgc gcgcacgttc cagaacatcc gcctgttcaa cgagatgacg gcgctcgaga
      181 acgtcatggt gggtcagcac tgccgctcgc acaccaacat gctgcgcgtc atcgcgcgca
      241 cccccggagc gaagcgcacg gagaaggcgg tccgcgacag gagcctggag ctggtcgact
      301 acgtgggcct ctccggacac ggcggggagc tcgccaagaa cctgccctac ggctcgcagc
      361 ggcgcctcga gatcgcccgc gccctggcga cggaccccac gctgctcttc ctcgacgagc
      421 cggcggcggg catgaacccg caggagaccg ccgccctcat gggcctcatc cgcaggatcc
      481 gcgacgaggg catgacgatc atcctcatcg agcacgacat gaaggtcgtc atgggcagct
      541 ccgaccgcgt cgtggtgctc gaccacggcg agaagatcgc cgaaggcccg ccggagcagg
      601 tccgcaacga ccccaaggtg atcgaggcct acctcgggaa gggagcctga tgggcgccgc
      661 ggccgcgacg gcggagaagg acggcggcgc gctcctcgcc gtgcgcgaca tccacgtgtt
      721 ctacggcaac atcgaggcgc tcaagggcgt ctcgctcgag atcaacgagg gtgagatcgt
      781 cacgctgatc ggcgccaacg gggccggcaa gacgacgacg ctcaacgcca tctcgggcct
      841 gctgcgcccg cggtccggct cgatccgcta cggggggcgc gacctcgcgg gggtgccggc
      901 ctacgaggtc gtcacgcagg gtctctcgca gtcgccggaa ggccggaaga tcttcccgcg
      961 catgtcggtg cgcgagaacc tcgagatggg ggcgttctcc cgccgcggac aggacctcaa
     1021 ggccgacttc gagcgggtct tcgacctctt cccggtgctc agggagcgcg agtcgcaacc
     1081 gggaggcacg ctctccggcg gcgagcagca gatgctcgcc atgggtcggg cgctgatggc
     1141 gggccccaag ctgctgatgc tcgacgagcc gtccatgggt ctcgcgccca tgctcgtcga
     1201 gaagatcttc gacatcgtca aggagatcaa cgcccagggc acgaccatcc tcctggtcga
     1261 gcagaacgcc cacgtggcgc tcgagatctc cagccggggc tacgtgctcg agaccggccg
     1321 ggtcaccctc tccgacgacg cctcggcgct gctgaagaac ccgcacgtgc gcgaagcgta
     1381 cctcggcgcc gactgacggt ctgcccgcga cggcaggccc cacgagggcg accggccgtg
     1441 cggcgccgcg tcacctgatc ctgaaggtca cccaccggct gcgcgccgcc gtgctcgccg
     1501 gcgaagccgc cagggaggcg gcggcgcgat aggcgccgcg gcgcggcgga gcgtacgacc
     1561 aggcgaacac gcccgccgtg gaggtgtcgc gagagaccgc cctgaccgtc ctccaggcgc
     1621 cgccgcgtcg ctgctggacg aagaccttca gcgtcgtgcc ggcacgaccg aggggctgca
     1681 gcgtcccgcc gagacggacc cggcgcccga gcgtctggat cgcagggccg gcgcgcagcg
     1741 tcagggacga cacgaccgtc acggtgaagg cgccggggcc ggcgcccttg ccgccgcgac
     1801 tgatgaccga cacggggccc gacgtcgcgc cgggcggcac cacggcggtg atccgcgtgt
     1861 cggagtcgac ggcgaacacc gccaggacgc cgttgaagct gacggcggtc gtgccggtga
     1921 agcggctgcc ggccagcgtg acctgggcgc ccggcagccc gaacgccggc gtcatcgccg
     1981 cgaccaccgg cggcggcacc gtgtggtgcg cgctgccggc cgcttcggag ggcgacgccg
     2041 agacggggtg acacgaggtg caggcgtccg acgcgccggc gtgcgcctgg tgtgtgacgg
     2101 tcgagccgtc gcgcaggtgg cacgccgcgg tgcaggccgc atccacgcgg gacgcgctca
     2161 tgtcctggcc cggggcgtgg caggtccagc aggtgaggtc cgtgtccggc acctggaagc
     2221 cggggtggca gccgagacag tgctcgttgg tcggcgtcca ccaggtgtgc gcgttgacgt
     2281 ggcacgtctc gcagtcctcg acggcgatgc cgccgtggat gtaggcgagc tgggcgaggg
     2341 cctcccgtgg agtcacggcg gccaggatca cgatggccgc cgcgaccacg agggcggcca
     2401 cggcgatgcg ggacatccga ccgcggtccc gccggctctt ctgacccatc gatcccctcc
     2461 cgtcagctcg tatgccgaag gtgcgggggc cgcggcggac tgacgtccgc cgcggccccg
     2521 tcttgtcacc cggcttcggc gccgggcgat gcgacgatgg cggtgccggt gactaccgga
     2581 tcctgatcgt catgtaggcg ctgtacttgt aggtggacga ggcgctcgag cggatccacc
     2641 tgaccctgta gttcttgtag gtcttggaga ccttgaacgt gaacgcgaag ttgccgctcg
     2701 cgtccgacag gctcgtgcgg aggagcttgg agcctctcat gagctggacc ttggcaccgg
     2761 ccgcagccga gttgtcgccg ttgacgatac ggccgacgac gtgggcgttg gcgcccttgt
     2821 cgacgggcga cttggcgccg gcgtgcatga acctgccgcc caccgacttc gccatctgct
     2881 cggccttgac caggccggcc atgatgtacg gcgggttgtg gacgccctcg gaggcatcgt
     2941 tctcgaacac cttgtagttc cacgtggccc ggccgacgag cacgtagccg ggcttggcgc
     3001 tgttggtggc ggagaactcg ctcttggtct tggccttctc gatcgccgcg gcgaccttgg
     3061 cggccttggc ctctgcagcc tcctgccact cgtcgatctc agcctgcagc gcgctgcggg
     3121 actggctcgg gtgacagccg gtgcaggagt cctcgccctg gtacgcggcg ccggccatgc
     3181 cgttccacct ctcggcgtcg cccgggagca tcggcttcat gccgtgcgag atacgggtgg
     3241 cggccttgtt ggtcttgggc atgtggcagt cttcgcacgc ggcgcccggc atgaactcgg
     3301 tgccggccgc ggtgtcgtac atcgcgctgc gaccgtggag ggtctcacgc tgcgggtggc
     3361 tcgggctgcc gtgggcggcg aggtcggcca tgggggccgg gctcggcttc tggccggtga
     3421 cctcgacctg ccagttgtgg cagtcctcgc agatgctggc gttgtccacg ctcaggcccg
     3481 cggcgctgcg ctctccggcc ttgtccgggt cgcgcacgac gtcggccgcg ccgacgcccg
     3541 acggatgccc gccgtggcag gtcgagcact cctggcccat ctggcccacg ttgtccttgg
     3601 tcaggacgag gtcagcggcg agcgaggagc ccttcgactg caggtacccc tcaccggtgt
     3661 ggcacttgta gcagccgccg gtgaggttgg ggtcgaagtt gttcgagtag tggccgttgc
     3721 cggcggcctg gaaggccatc gcgtccgggt cgtccttggt gagagcggaa cgatacgaat
     3781 gcccggaggc ggcccactcg ttgtagtagt agtggttgcc cttggcgttg ctcccgttgg
     3841 ggaacatcca gtacgccgcc gggctggcgg cgaactcttc ctcggtcggg atcttggtgt
     3901 acgactggcc gccactgacg ccgttgatgt cgacgaagtc gcgcagttgc aggttggtgg
     3961 tgtagccgta gatgcccatc gtgccgtcga cgctggtgta gctgccgtgg cactggccgc
     4021 acacctgcga cttgccgagg acatcgatgc cggcggagac gacgacgccg ctggtgaagt
     4081 gcccggtggc cgacgcctcg ccggtgccgt ggcactgctc gcactggatg ccgaggcccg
     4141 gcgtggagac gctggcgtag tccatgaagt cctggaccgt cgtgctctcg tcacgggccc
     4201 actgacgcgc ggtgccgggg ctcggcgcga tggtcgcggc ggggttcggg acggtgccgg
     4261 tgcccggcac ggtgctgccc atcatgtggc agcgctggca ggcgtagctc tcatcgaaga
     4321 gaccggtgcc gggatctcca gcggcgacca tccactcgcc gccgtgcgcg ggctcccacg
     4381 agaggccctc gacgatgttc cacggcatca cggtcgggtc ctcggtaccc ctgaagaaga
     4441 ggtactcggt cgccgagttg ccgacctcgc tggagtcacc gagggtgatc cagtcgagat
     4501 cggtgatgct gtagctgccg ccggcgtggt acatgctctg gccttctccg gccaccggct
     4561 cgacggcgcc ggggccctgg aaggccgtcc agccttcggg aacgtccggc aggatgcccg
     4621 actgcgcgaa gcggccgtgg agggccagtt cgctcgggcc catgatgcca ccgtggcagg
     4681 tcagacactt ggtctgaccg gcgtagtgca tcccgtattc gttcacaagt tgcgcggacg
     4741 ccagggcggg cagcagaagc atgcacgcga tggcgaccgc ggtcagaatc gagactttct
     4801 tccccatagc tcctccgtac tggtgacaca gcagagtggt tcggcctcca cacaccccct
     4861 tgccctggtt gccggcaggg cgtcggcggg acacgggaca cgggacacgc tcgcggaatg
     4921 tgggcgcacg gacgacggac cgcgcgacgg cggccccggg cttctgccgc tcggagtgct
     4981 cccttcagcg agcgtggccg atccccaaga accccggcga cactcgcacg gaagtctagg
     5041 gtgaaacacc tagacataca agcgaaaaaa ctgcagtcac ctctacaggt ttgttcgtag
     5101 caggcgctgc agcggacagg tggtgaggcg gcagaggtgt cccccaggcg tctgacgcca
     5161 tccacgatgc cacgcacgac gtcaccggtg acgccaccca tgacgccact cgtcctcgcg
     5221 gtaccccgcg cgtggccggc gtggcgcgag cgggcgtggc gatgcagccg ggccggcgtc
     5281 agccggccga gccgcaccag cgcagaagca tgagcgccac cgcggcggcg tggtcgacga
     5341 ggtcggcgac cggtaccgac tcgtcgatgg tgtgcgccct ggcgaggtcg ccgggaccga
     5401 acgagaccgt cggggtcccg gcgacccggg tgaacacggc cgcgtcgtgc cacgagtcga
     5461 ggccgctgat gcggccgggc cgcccgacgt cggcggcggc ggccagcgtg ccggtcacga
     5521 tcggggcgtc gtccggcacc tcggcgggga cgatgtcgca cggccactcc cactcgggcg
     5581 ggtgctcggc cagccagtcg tcccggtcgg cggcggcctt gacccaggcc tccacctcgt
     5641 cgaagacgtc ctggccgccg cggtcgccgt ccacgcggcc cggcatgtac tgcaccgaga
     5701 gcaccgcctc gcacgaggac gggtacgtga ccatccactc cccgcccctg atcacggtgg
     5761 gcagcacgtc gggcacgtgc aggtacttgt gctggtgctc ggggcggcgc gcccagtccg
     5821 cgcgcagctc gtgcaggctc gcgagcacga cctccatctt ctcgatcgcg ttgaccggcc
     5881 cgccgtcgcg ccagtgcggg tggtgcatct cggcgtgccc ggtgcggccg acggtgcgca
     5941 ccaccgggtt gacgccgccg cggcaggcga cccagacgtc gaaggcggtg ggctcggcgc
     6001 acaggccggc gtcggcgcgc acgccgtggg cggcgcacgc gtaggcgccg gcgccgctcg
     6061 actcctcgtc ggtgtccgtg cagaagacga cgtcgccggc gagtcgcgcg cccagccggg
     6121 tcacggtctc gagcgcgaac agcatgccgg cgatgccgcc cttcatgtcg cagctgccgc
     6181 ggccgtagag ctggccgtcg cggacctccg gccggtgcgg gtggctcgtc caccggtcca
     6241 gcggctcggc cgagacggcg tcgatgtggc cgttgagcag gagtgagcgg ccgccgcccg
     6301 cgccgcgcag gcgcgcggcg agctgcggcc gcccgcggaa gtccagcccg tccggcacca
     6361 cgcgattgcc cgtgccggtc ggctccggct cccacacctc gacctcggcg ccgagcgcgg
     6421 cgaggcggcg gcgcaggtgc tcctgcagct ccgcctcctg ccgcggcggg tcgccgaggt
     6481 tgcgcgccgt ggtgtcgaac gccacgaggt cgctcgcgag cgccacgagc tccggttcgc
     6541 cctcgcggac ggcggccacc acgcgttcct cgaacgccga gcgaagggtc tggctcatgg
     6601 ctcctcctcg agagctggat gtggtcgtac gcgccgccgg tcgtacggcc gggcgggccc
     6661 gagagcccgc ccggccggtg tgtcagaggg cgcccggcgt caggtcggag tcactccggc
     6721 ggcagctcgc cgaaggtgat gctgacgtcg atgccctgct tcttgctgcg ggcccgccag
     6781 aagaagtacc aggccaggcc cgcgacccac gccgcggcga agacgaacag gttcttgccg
     6841 gtcacgtcac gcgaggtggg gtcgatgaac gagaagtaga ggaacgcgaa gatgaagatc
     6901 aggtagagga ccgctccgat gctcacgagc gggatgccgg cgatcttcca cgtcctgtac
     6961 ggcgacgcct cccagatgcc gcggacgttc ttgcggtacg ggaagagcag cgccgagatg
     7021 ccggtgacca tgaacacgga gatgaactgc atgccgccgc cgatgaggcc gctgaggtcg
     7081 ctctgcagcc agagcacgta gacggcgacg cccaccgcga acacgacgag ggcgaacgtg
     7141 aggttcttga tcggctgcgc gtactggggg ctcacgtcgg tgaaccactt gggccccatg
     7201 cggtccagac cccaggccag gaacgcacgc ccgatgatga ggatcgcgat cgtgatctcc
     7261 agccagatca cggcgatgaa cccgagaagg atcgcccacg ccacccagcc gctctggccg
     7321 ctggcgatcc acgagagcgt gaggtagtcg ggagagtagg gggccgtgta gccctcgacc
     7381 ccggccccga agctcgcctg cgccgtcgcg gagatgaagt tgaacgccgc cacgcggtac
     7441 agcccgagca ccgtgaggac gccgaggacg atgggcaccc agacggcgag gatgttgccg
     7501 cccatgaggc tcttgtccgg gcgcttgacc tcgccgctca ggtacgcgat ggcgtagttg
     7561 tagacgaaca gcgcgtagac gccgctgagc gcgccgaggg tgccgtgcca gtcccaggtg
     7621 gtgggcagcg ggccggccgc ctggatgaac gcctcgtagt cgagtgagcc gaactccgcg
     7681 gcgatggcgt tccagttgcc gatgaactcc gccttgctcg agaggaacag agcgatgatc
     7741 agcacgatcg ggccaccgat ggccgggatg agcaggatct tctggatccg cgagtagatc
     7801 ttcatgccga acgcgaccga gaggtagccg atgacgacgg cgatgaggcc gagcacgaag
     7861 aggccggtct tggaggccgc ccagtcggcg aagccagtcc agcccatgta ctgcccgaag
     7921 agctgcagag ccggcacgcc gagccaggtg gcgatgaacc agttccagta gaagatggcg
     7981 aggatgttcg cgaacaccgc cccgagcgcg atggccgggt gcaggatgcg cgagttgtag
     8041 acgtactcgc cgccggctcg cggcatggag ccgctcagga tgccccagac gagaggggcg
     8101 ccgatcccgg cggtgacggc gccgagcgcg atggcgagga gcaggttccc cgtggggaag
     8161 agcgagagcg cgatacccac catgagccac agcgtggcga tgggctgaca ggtcatgagg
     8221 ccggctccga acgcgtccca catgtcgaga ccgcgcacga ggccgctggc tttgcgtgcg
     8281 aagacaagat cttcggccat acgtccccct ggaatgacgt aggtagcgaa cgtcacgagc
     8341 cggcgcggcg gcggcccctc cccgcgccgt cccactgcta cgccatcaca ccacctcccg
     8401 tcgtgcagcc gcccgtggcc gcgggctgca taagatgcgg aagaccttca tccgaatgct
     8461 acgggcaaga ctcagggtgt caaggaccgt ttcgctccag aagagcgcgc gagctcggcg
     8521 ccccacgcgc gcgcccgctc cgcctctccc ttcctgagcg ggccctggga gcccttgacg
     8581 acgaagccct gcggctccgc gacgcgggcg tatcctcgcg cctcgagcag ctcgaggatc
     8641 tccggcgctc ccttgccgaa cgggccgcgg acctgggtgt cgaacgcggc gcagcccgcg
     8701 ctgccgtgcg gcagctcggc cagccaggcg cgcagcaagg ggcaggagag gtccggagcg
     8761 ggtcccttgc ccgggctccg gcggatgccg tcgcgcatct tctgcgagga gagcttgaag
     8821 ccgaacacgg gggcgccggc gacgacgagg tccgcgcccg cgatgtcggc cgcggaggcc
     8881 tcgtcggtgc gcagggcccg ggcgcccggg ccgagaccct cggccaccgc ccgagccacc
     8941 tccgccgtgt tgccccacaa ggactcgtag accacgatcg cattcatctc ccgccctttc
     9001 tcccggacgc cgcctccggc gcccggctgc gccgctcccg acgcggcgat ggtacgacgc
     9061 cggcctcgct tcgccgccgc ctccgggggg tagtacgact cccgagacac tgattgcgga
     9121 ggaggggcga gcatggctgc ggaggaacga ctgcgggacg cgaccgtgac ggcgacggag
     9181 ttcgacctgc gggccgccga ggcgccgccc gcgcgggcgc gcgcggtggt ggtcggcggc
     9241 ggcatcatcg gcgcctccat cgcgtaccac ctcgcccggc tgggctggac ggacaccgtc
     9301 gtcctcgagc gcgcccgggt ctcgagcggc accacctggc acgcggcagg gctgatgacc
     9361 cgcacccgcg gcacccacgt ccagaccgag ctcgcgagct acagccgcga tttctacgag
     9421 ggactcgaag ctctctccgg cgtcgacgtc ggctattacc ccaacggctc actgtccgtc
     9481 gcccgcaccc ccgagcgcct cacggagctc cgttacgcgc tgtcgctggc gcggcaccac
     9541 ggtctgccgg cgcgcgagct cacggcggaa gggatcgccg gcgtctcgcc gctgctcgtc
     9601 gtcggcgacc tcgtgggcgg ggtgctcttc gaggaggacg cgacggtcaa tccgggctgg
     9661 gccgcctacg cgacggccaa ggcggccttc gacctcggcg cacgtctcgt cgaggatgtg
     9721 caggtcaccg ggttccgcct ccgcggcggc cgcgtcgtcg ccgtggagac cgaccgcggc
     9781 gccgtggagt gcgagatcgc cgtgatcgcc gcgggcctct ggtcccgcga cctcggcctg
     9841 ctcgccggcg tccggctggc gctgcacccc tccgagcact actgggcgca gacggagccg
     9901 gtggacggag ccacacgcga tctgcccatc atccgcgacc tcgacggctc catctacgtg
     9961 cggcactacc ggggcggcct gctcgtcggc gccttcgagc ccgacgggcg accgcgcacg
    10021 accgcctcca tcccgcccga cttcgcgttc ggcgagttcg agccggacct cgagcacttc
    10081 gcgcgccctc tcgcccaggc gacggtgcgc gtgccggcgc tgggacaggc gcgcttcgcg
    10141 cactacctgt gcgcgccgga gagcttcacc cccgacggca acttcctgct cggcgagacc
    10201 gccgaggtcg ccgggctcta cgtcgccgcg ggaatgaact cgcagggcat catcctgggt
    10261 ccgggcgccg gacgcgcgct cgcggagtgg atcgaggccg gcgccccgac ggtggacgtc
    10321 gccgacctcg acgtgcgccg cttctcgccg gcacagggca acgcgagcta tctgttctcg
    10381 cgcacgcgcg agtcgctggg caggctgtac gcgatgcact ggccgttcct gcagcccggt
    10441 acggcccgcg ggctgcggcg cgtgcctctg tacgaccgcc tcgccgcggc cggcgcctgc
    10501 ttcggcgaga cggccggctg ggagcggccg aactggtacg cgccgccggg cgaacgcccg
    10561 gagtacgtct actcctacgg ccgccagaac tggttcggcg cggtcgccgg cgagcaccgc
    10621 gccgcgcgtg agggcgtcgc gctgttcgat ctctcctcgt tcgccaagat cagggtggaa
    10681 gggccggagg cgctgcccac cgtgcagcgg gtcttcaccg ccgacctgga ccggccgccg
    10741 ggcagcgtcg tctacacggc gatgctcaac gcgcgcggcg gggtcgagct cgacgcgacc
    10801 gtcacgcggc tcgccggcga cgcgttcctc gtcgtcacct ccgcggcggc gcagcacaag
    10861 gcgttccagt ggctgcgccg caacgccggc gccggagccg tcgtcaccga cgtcacctcc
    10921 ggtctcggcg tgctctcggt gacgggaccg cgtgcgcggg acctgctcgc cggcctcacc
    10981 gaggccgatc tgagccccgc ggccttcccc ttcgcccgcg cccgtgagat cgacgtgggc
    11041 tgggcgaccg tgctcgccct gcgcgtctcg ttcacgggcg agctcggctg ggagctgtac
    11101 gcgccggtgg agtccctgcc ggtcctgtac gaccagatcg ccggcgccgg cgcggagcac
    11161 ggcctgcggc acgccggcta ccacgcgctg gacgggctgc gcgccgagaa gggcttcgtg
    11221 cactggggcg cggacgcggg gccggccgac acgccgcccg agaccggcct cgaggccgtc
    11281 gtcgccccgg acaagcccgg cggcttcatc ggccgggagg cgctgcaggc cgccggccct
    11341 ccgcgccgcc ggctcgtgcc catcctgctc gccgacccgg agccgctgct ctaccacggc
    11401 gagtccgtgc tgcagggcgg ccgcgtcgtc ggccgcgtca cctcgggagc ctacggccac
    11461 accctcggcg cggcggtagg cctcgcggcc atcgccggcg agcccggtca gatcgacgcc
    11521 atcgtcgccg gcggcgccgt ggaggtggag atcgcgggcg agcgcgcgcc ggcgcggctg
    11581 agcgcgcgcg ccttctacga cccggacggg ctgcggctgc gcggttcctg acgaccggcc
    11641 ctctctcggg cggaggcgta ggatatcggc gtgagtcgca tgctcccgca gaagtgggcc
    11701 acgacccgcg ccggcctggc cccggcgctg ggcatcgccg gcgccctgtg cctgctcgtc
    11761 gccggcgtca tcggcatcgc gtcggcgcag ggcggtccgg cggtccgcgc ctcctcctcg
    11821 ccctcgccgt cgccctcggc ctcggcctcc cccggcgtca gcgagaccct gaccacggcg
    11881 gtcgtgcgcg ccgtcacggt acaccgcggc tcggtcgccg gcgtgcgcta ccgcgcggac
    11941 gacacggccg gcggcacggt gaccctcgac ctgctcatca cgacccgcgc gggcgcggtg
    12001 gtccggcgcc tggcgaccgg gcgcgtgacc gaggtcggcg tggaacgcgt gtggcgcggt
    12061 cgcctgcggc tgcgccgcgg ccgctacctg ctcgtcgcgc acgccgtgga cgcgagcggc
    12121 cgctccgagg cgcacgcccg cacggcggag ctccgcgtgc tcgcgccgct ccccccgctc
    12181 gtgcccacgg cgagcgcccg ccgcgccgcc ttcgcctggg cggcgcggcg cgccggagac
    12241 gtggccgtcg ccgtcgtcga cagccgcggc cgtgcgtacg gctaccgcga gcagcgctcg
    12301 ttcatcaccg ccagcgtcgt caaggcgatg ctgctggtcg cgtacctgcg ccggcacgac
    12361 acggtcgacc cggtgatgcg cgcgacgctc acgcgcatga tcaccgtctc ggacaacgcc
    12421 gcggccgacg cggtctaccg gagcgtcggc cgcgagggcc tcgcgcggtt cgccggccgc
    12481 gccggcatgc gctcgttccg cgcgagcggc gcctggatcc tctgccgtgt cgccgccgcg
    12541 gacatggcgc gcttcttccg cgacatggag cggtacatcc cgagccggca ccgcagcttc
    12601 gccaacggcc tgctggccgg cgtcgtctcg taccagcgct ggggcatccc cgcggccgcg
    12661 ggaccgctcg gctaccgcgt ctacttcaag ccgggctggc tcggcgcctg ggtcctggcc
    12721 agccaggctg cccgcctcga gcggcgcacg gtccgcatcg gcctcgccgt cttcacggac
    12781 cgcaacccgt cgtccacgta cggcaaggag acggtggccg gcgtcacggc gcggctgctc
    12841 cggcgctgac gcccccgatc acgccgccat cccgcgccgt ccgttccgct cgtcgcgccg
    12901 cgagcgcacc tcgttgggcg actccccgcc ctcctcgaag tcgcgcacgt tggcgagcgt
    12961 ggtgcgcgcg atctgctcca gcgcctcgcg cgtgaagaag ccctgatggc tggtcacgat
    13021 gacgttgggg aaggtgagca gccgtgagag cgcgtcgtcg tcgatgaggc ggctcgagta
    13081 gtcctcgtag aagtacagcg cctcctcctc gtagacgtcg agccccaggt ggccgatgcg
    13141 tcccgacttg agcccctcga tgaccgcgcc ggtgtcgatg agcccgccgc ggctggtgtt
    13201 gatgagcatc acgccgggct tcatgccggc gatgctccgc cggtcgatga ggtggtgcgt
    13261 ctccgccgtg agcgggcagt ggagggtgac caggtccgat gcgctcagca gcgcgtcgag
    13321 gcccaggtac tcgaccccca tccgccgcag gcggtcgctg ggggacagat catgcgccag
    13381 cacgcggcag ccgaaccccc gcagcagcgt ctcggcgacg atcgcgccga tcttgcccgt
    13441 gcccacgatg ccggcggtct tgccgtgcag gtcgaacccg aggagtccct ggagcgagaa
    13501 gttgccctcg cgaacgtgcg tgtacgcgcg ggagagatgt cggttgagcg ccaggaccag
    13561 ggcgatggtg aactccgcga ccgcgtacgg cgaatagtcg ggcacgtgca tcaccgtgag
    13621 gccgaggtcg tgcgcagcgc ccagatcgac gttgttgaac ccggcggagc gcagggcgag
    13681 gagccgggtc ccgtgcgcca cgaggtcggc gagcacctgc tccccgaggt cgtcgttgac
    13741 gaaggcacag accgccgacg acccgtgcgc cagccgcgcg gtcgccggct ccagccgcgc
    13801 gtcgaagaac accagctcgt ggccgaagcc ctcctcgtcg ttgacgcgcg cgaggaactg
    13861 gcggtcgtgg ggtttgctgc tgaaaacgga gagtctcact ggagaggacg ttccccggcg
    13921 cggagcaagg cgaacccgac tacgacgttc atcacacccg acgaatgttc tggcgtctcg
    13981 ggccggcgtc cggccgatac cggaagtgaa gccggcgcga gaggcgccgt ccgttcggga
    14041 gcagtgatga gcgccaagcg tctggcctac agccgatatc agatcctctg cgccgagcgc
    14101 gacgcctggc tcgaggccga gacgggggac acggacgcgt actactacga cggcatcagc
    14161 ttctggcgcg tcgccggcga cggcaggaag cgcctcgtcc cgtcctggcg ggctcccgcg
    14221 cacggctggc ggcacgacga ggactgcacc tgcgacctct gcaccgcgta ccggcctgcg
    14281 aggctgcgcc cggcctccgt cgcctgagcc cgagggaggc tacacgaatc gggcgccggg
    14341 gcggaggccg ctcgcggctg cccactcgcc ggcgcccgtc ttgacgccgc cctccgggcg
    14401 cacccgggtc acgcgcaggc ggccgtcggc gcacacgacg ctgaacccgt cctcgccgac
    14461 gtcgacgacc tctcccatcg cgccgccgac gcccgcgacg tcgcgggcgg ggcgcggcgt
    14521 cgccgcgacc acctgcaggg cctggccgcc gctggtggtc caggcccccg gtgccgggtc
    14581 acagccgcgg accaggtcgt gcagctgccg gcacggcttg ccccagtcga ggcgcgcgtt
    14641 ctcccgctcg cagggcgcct cgtagctggc cagcgactcg tcctggggca ggcgcggcgc
    14701 ggtccccgcg ttcaccaggt cgaccgcctc gagcagcgcc tcgacgccca tcgggaacag
    14761 gcgctcgaag tagaccgagc cgagggtgtc gccggcggcg atgggggtgt gcttctgcag
    14821 caggatctcg ccggtgtcga tgccgccgtc cgggcagaag accgtgaggc ccgtctcggt
    14881 ctcccccttg atgatcggcc agttgatggc gctgcgcccg cggtgtcgcg gcagcagcga
    14941 ggggtgatac tggatggtcc cgtacgcggg gacggcgagg aactcggccg gcacgatcag
    15001 cgtgacgaaa gccatcacct ggaggtcggg cgcgaacgcg cggaactcct ccgtcaccgc
    15061 ggggtcgcgg aacgacgccg ggcggacgag cggcaggccg gcggcaaggg ccgcctccgc
    15121 gagcgggtcc tccctcttgc ccgccggcga ggcgtagacc gcgacgacct cgtcaccgcg
    15181 cccgagcagc gcctcgagca cggccttgcc gaaggcctgc tggccgtgca ccacgatccg
    15241 catcggtccc tcctcgtcgc ctgcgccgcc ggcggccgcg gagggaggcc gcgactgcgg
    15301 ggcactgcgg atgctacctg aagggcgttc gccggccgag cgggtcacga ggccatgacc
    15361 gcgcggccag gctcctccgg gcgagcccgg acctgctgct tcgcggtggc ctcgccatcc
    15421 ggggcaagcg gccggcatcg tcggcggagc cgccatgcgc ggcgcgcgga acatggcgcc
    15481 cgccccggcg cgcagcgacg ccgggtctag aagcggccgc cgatccaggc gccgctgatg
    15541 gagccgtagc tcaccacggc gcccgtgtgc ggggcgtgga tcatgctgcc gccgcccgcg
    15601 tagatgccca cgtggtggct gtagctggcg ctgccgaaga acacgaggtc ccccggctgg
    15661 agggcgctca gcgagaccgg cttgctctgg cgctgctggt cggtcgcgcc gtgcgacatg
    15721 ccgatgccga tctgcgcgta gcagtacatg gtgaggccgc tgcagtcgaa gccgctcggg
    15781 ctggccccgc cccacacgta gggcacgccc agatagcgct gcgcgatggc gacgacggcc
    15841 gagcgccccg agccgccggg gtcgatgacc gtgccgccgc cggagcccga tgaggacgat
    15901 ccgccggaag aggagccgga ggagctgctc gcggccgagg acgacgcctg cgaggccgcc
    15961 agcgcggcgg ccctctcggc ggccttcttg gcggccgcgg cggccttggc ctggagctcg
    16021 gcgatctcgt ccttgaggcc gccggccgtg ctctcgagct gggcctgcag cgccagcacc
    16081 tggtccttgc tctcctcgcg ctgggccacc agcctgaccg ccgacttgcg gtcctcctcg
    16141 agggccacgc gccggtcgga gatctcctgg cggtacgccc tggcggcgcg cgccgtccgc
    16201 gcgtcgctct ccgtcatgcg ctgcatcatg tcgatctgcg tgaggaggtc ctcgaacgtc
    16261 cgtgacgcga acacgacgtc gaggatgccg gcgtcgtccg ccttgtagac ctggcgggcc
    16321 cgggacgtga ggtggtcgtt ggccacgccg agcttgtact cggcgacctt gagcaggtgc
    16381 tggttgcggg tgatgcggtc ctgcacgtcc ccgagcctgg aggccgcctc gttgtaccgc
    16441 tctaccgcca tgtctgactg ctcgtagacc ttgctgagct gcgcctggac ggccgcgagc
    16501 ctggccttct tgtccttgat ggggccggcc accgcggtgg tggcgaagac gagcagggtg
    16561 aggagcgaga cgagaccgag gaaggtcgcc ttgcgcatgg gggtcaagcg gatcgcctcc
    16621 ttcgcgccgg tggactcatg gtccctggcc cggcgccggg cttgagcttg gcacggtagc
    16681 acccggcgcc gcccggggca aaccgggcga cgttctgacg cgggcggatc gggtcgtgcg
    16741 gcaggcgagc cggcatctcc gcgcgtcagc tccggttgta gaagccgggg gagcgcacgg
    16801 cagaggccac gtcgccgtgc acgtccacgc tgtgcgccgc gatcagcagc acgtccctgc
    16861 cgacgtgctg gagcgcgccc tcgacggcgt aggcgaggcc gctgcagaag tcggtgaggc
    16921 ggccggcaag gccggggtcg cagtcgtgga agtcgacgag gacggggata ccggcacgga
    16981 cgctgtcggc gatggtctgc gccgcctcga agctctccgg cttgaccagc tcgaacgccg
    17041 gctgcgggtg gctcaccagc tccaggggac gcggcttcgg ccagcggtcc cacccggtcc
    17101 cggcgtcgtc gccggtatcg tggtcgtcgt gccagtggtc gtgagggtcg cgcgcagaag
    17161 ccatgcttcc tccggtgggt cggatctgca tccaccttcc ttcggcgcgg cgcatcggtt
    17221 cgcctgcctc acgcccgctt ccgccggcgc gcggtagccc ttcagaggct ccgacctcgg
    17281 cgctgcgtca ccgttttcgc gcactgagac cagggtaggg gataactcct ctccgaggct
    17341 ctgacagccg atcgacgccg aaggaggccg gatgccggac ctggatctcg tgctggttct
    17401 cgcctgcgcc ctcgtgctcc tgagcatcct ggtcagcaag gcgtcgctgc gcaccgggat
    17461 ccccgccctc ctcgtgttcc tcgccatcgg catgctcgcg ggatccgagg gactcctggg
    17521 catctacttc gacgacgcct ggctggcgcg cgccgtaggc gtcgtggcgc tcgcgctgat
    17581 cctgtacgcc ggcggcctgg acacgcgctg ggagagcatc cggcccgtcc tcggccgcgg
    17641 cgtcctcctc gccaccttcg gcacgttgct gacggccggg ctcgtcggca tgctcgtgct
    17701 gtggctcacc gacctgccgg tgatgcaggc gctgctgctc ggagccgtgg tgtcgtcgac
    17761 cgacgcggcg gcggtgttct ccgtgctgcg cgcgcggcag gtgcgtctgc ccggcgacgt
    17821 ccgctccatc ctcgagctcg aatcgggcac caacgacccg atggcggtgt tcctcaccgt
    17881 cagcctgatc gcgctcatca ccacgggagg cggatcaccg ctcgacctcg ccgtctcctt
    17941 cgtgctccag atgggcatcg gcctgctgtt cggctacgcg ctcggccggg ccatggcctg
    18001 ggcgctcgac cgcatcaacc tcgagcacga cggcctgtac ccggtgatga gcctggctct
    18061 cgtgctcctc gccttcgggg ccacgcagta cgtgggcggc agcgggttcg tcgccgtcta
    18121 cgtcgcggga ctcgtcatga gcgggcgcgt ctacctgcac aagcgcagcc tggcgcggtt
    18181 ccacgacggc ctcgcctggc tggcgcagat caccatgttc ctgacgctcg gcctcctcgt
    18241 gttcccgagc aggatcctcc ccgtgctcgg cgtcgcagtc ctggtgaccg cgttcctgac
    18301 gctgatcgcg cgaccgctgg cggtgtacgt ctcgctcatc cgctcgcggt tcacgatgcg
    18361 cgagcggacg ctgatcgggt gggtgggcct gcgcggcgcg gcgccgatcg tgctcgctac
    18421 gttcccactg gtggcgggca tctccggcgg cgacacgctg ttcaacatcg tcttcgtcgt
    18481 ggtgctggcg tcgaccctga tccaggggac ctcgatcccc atggtcgcgc gatggctggg
    18541 gatcgagggg ccggcattcg agtcggagcc ggcctgggcc ggcgagccgg tgcggcgcga
    18601 actggtcgag ttccgggtgc cggagacctc gccgctggtc ggccggcgcg tcgcgcaggc
    18661 cggcctgccg gccggcgcgg acgccgtgct catccatcgc tacgacagct acctggtcgt
    18721 gcgcggcagc acacggctgc gacgcggcga tgtcgtcctc ctgctcgccg atgagcccgc
    18781 gctccgcgag ctcgaggccc gccgcgagct cgagcgccgc cccacgctcg ccgcctgcga
    18841 gaccgacctc accgcctgag ccggcgcgcg ggcgggcggc gcggcgtcaa gcgcagggtg
    18901 gcgccggcgc ggcgtcaggc gcagcggatc acgccccgcg cccacaggca gtcgccgcac
    18961 gcgggatgcg ggttgccgaa gcagtcctcc tcgttggcct cggccagttc acacccgcag
    19021 tccgtgcacg gcgggaaggc gaagcgccgg acccggtcgc ggaacgcgac gtactccggc
    19081 ttctcccaga tgcccgccag cgtctcgtcc ggaaggcggc ctgcctccca gcgcagcatg
    19141 cgcttctcgc gcccgcgcac gaagcaggtg taggtgtgca gcagtggcgg gcacgggctc
    19201 acgcccccgt gccaggccac ggcgcaggcg tccgcctcga cgaaggggca gtggctgcgg
    19261 gtcgcctcgg tctcggcgcc gaagaaggag accggcccgg agcgggtgag cgcctcgccg
    19321 agcgctgcac cgagctgccg gtcccagtcg aacgccggca gctgccagcg cggcgccgcc
    19381 gtcgtctccg cgccgctgag cgagctcgcg tggtggtcgt agagcgtctc ggcgagcagg
    19441 tcttctgtgt acgcgagaac attgctgacg atgatgaacg tcgcgccgag ctgagcggcg
    19501 agccgaccca ggcccgggag ctcctcgacg ttcgagcgca tggcgacgaa ctcgacgccg
    19561 atgcgcagcg aagggccgta gttcggcccc ttggcgtcgt gcagccgacg cacgttctcg
    19621 acgacgcgtt cgagcgaagc tccggagcgc acgcggccga aggcttccgc ggaggcgccg
    19681 tcgatgctga ccacgagctg gtcgagaccg gcctgcagaa gtccggccgc catctcgtcg
    19741 tcgagcagca gcgcgttggt ggtgacctcc gcgcgcagtc cgctctcctt gaccttccgc
    19801 accatgtcga ggaaccgcgg atgcagcaga ggctcgccga ggcccatgaa ggccacgccc
    19861 ccggcctctg aggcaggcgc cggcgcgaga ccgtccacga ccgccgcgaa cgtggcccac
    19921 tccatgaagc cctcgggctc gtcccaggaa tggcgcacgc acgtcctgca gtcgaggttg
    19981 caggcgttcg tcggctcgac gtagaccttg cgggggccct cgaccgtcgt catgtccgcc
    20041 ttctcaggcc ttccgcttcg ccgcccgcag cgcgtcgagc gtcgggaaag tcagggctcc
    20101 tgcctcgcac atgccggcgc agtgcgagca gccctccatg caggatgcct tcgccacgac
    20161 gcgcacgtga ccgtcgacca tcgcgtagac gccctgccgg cagaactcga ggcagaccct
    20221 gcagtcggtg cagatcgcct cgtcgagggt cggtccccag acgaatgcag tcacgctggc
    20281 ctccctctca gcagcagcag ccggagccgg ccgcgggcct cttgttggcg aagtagcgct
    20341 gcaggttctc gggcagctcg gcgagcggct cgggagtgat gggcggcgac tcgtccagct
    20401 tgagcatcgc gtccatgagc ttctcgacga ggagcggcag cggggcggcg ctgtagacct
    20461 gcccgcggta gtgccactcc cggcagtcga cgctgacgtc gttgtcggtc aggtcgccgc
    20521 aggattcgca ctccgactcg cggatgtcct ggtcgatgtc gcgtccgttg atgcggatgg
    20581 tcggtgagga gagaagcgcg cgggccttcg cctcctccgc cgtctgcacg acggtctcgc
    20641 gataggtgag cccgatgccc tgggcctcgg ccgcgggggc gacgagctcg atggcgttgc
    20701 gcagctgctc tcccgtcggc acgcagcgcg cgcagctctc gaggtcgatg acgagcagct
    20761 cgatgttcag ccggcgtggc ggcgtcgcat ccgcgggcgg cccggccgcg gcctgggcgg
    20821 tgggggcgcc gccgcagcac ggagaggcgg cggactgatc gccgcagcag gacgaggtgg
    20881 gccgggcggt ggtctgtggg tcgctcatgc tggttcagaa ctccttcatc gaggtggtcc
    20941 ttcggatgtg tcgatcggga atggcgggtc acaagaccgc gttgaacagg aagccgacga
    21001 acatgatgcc cgtaccgacg acccccagga aggcgaagat cagcggccac ttcagcacct
    21061 tcttgaggat gatcgtctcc ggcagcgaga gggcgatcac ggccatcatg aaggcgagca
    21121 cggtacccag cgcggcgccc ttctcgagca gcgcctcgac gatggggatg atgcccgcag
    21181 cattcgagta catggggatg ccgaacagga ccgccagcgg cacgctccac caggcgtcgg
    21241 cgcccatgat cgacgccatg aagtcctcgg ggacgtagcc gtggatgacg gcgcccacgg
    21301 cgatgccggc gagcacgtac ggccacacct tgccgacgat gtccctgacc gcctgccagg
    21361 cgtaggagac gcggccgggc cagtcgagct cctgtgcgcc ggcggtgtcg cccatgcgga
    21421 tctcgcgcac ccagtcctcg acgtagcggt ccatgcgcag gcgcccgatc acccagccgg
    21481 cgacgatggc gatgagcagg ccggtgacga ggtagagggc ggcgacctcc cagccgaaga
    21541 gcccgtacag cagtacgagg gcgatctcgt tgaccatcgg cgccgcgatg aggaacgaga
    21601 aggtgacccc gagcgggatg ccggcctcga cgaagccgat gaacagcggc accgccgagc
    21661 agctgcagaa gggagtcacg atgccgaggc cggcggccat cacgttcccc gtggtctccc
    21721 ggcgcccgcg caacagcgac cgggtgcgct gcgggctgaa gaacgagcgc acgatgccga
    21781 cgccgaagac gacgatggtg agcaggagca tcaccttgac gctgtcgtag acgaagaact
    21841 cgagcgccga ggcccagcgg gtgtccggga ggctcagcac gtcgtggaag agccagacga
    21901 tgaacggcag ccagtagtga tagacgagcg cccagagcgc gacgccgccg gctactcgcg
    21961 cccaccacgg caggcggccg agcgcgcgag cgccggccct gaacggggcg cgccggccgg
    22021 cgaccgcgtc cgcgtccggc ggctcgctcg cggtggccgg cggggctgcg cactcgcagg
    22081 ccggcacgac ccgggccggc gcgtcgtccg ggtccggacc ctcgaccacg gcctgtcccg
    22141 ccgccgctgc cacaggctcc tcgagcgcct cggccgtcac gtctcgacgc ctccccgctc
    22201 cggtcccgcg aagctcgtga tcagcggcgt cgcggccagc tcaagccgca ggcgctcgtg
    22261 gtcgtagggg acgcgcacga actcgacgaa gccttcggag ccgagcttgg tcgggtcgac
    22321 gatcgcgtag cagacgcgcc agtcgccgtc cttgggccgg ccgccgctgc cgacgttgac
    22381 gaagaccgtc tcgccgaccg gccgtgcgta ggggacgtgc gtgtggccga agaggatggc
    22441 cggatagggg aactcgtcgg ccatgcgcgc catggccttc tccggccggt cctcgaagag
    22501 gtactcgttg atgcgccggg ggctgccgtg cacggcgagc agcgtaccgg ccggcgtgtc
    22561 gagctcgaag cggtcgtcga gcatgcgcag gtacgccttg acctcctcgg tcaccgcctg
    22621 ctgcgtccag gcgagcgacg ccgcgccttc ggcgcgctgc tggtccgtct tgtagacgca
    22681 gccgcagtcg ccggtctcga acgcgatgcc ctggtcgtag ttgcccatga gcgaggggat
    22741 accgcgctcg cggaccagca gggcgacctc gttgggggag ggtccgtagc cgacgaggtc
    22801 gccgagggag atcgtcgccg acaccttgcg ccgatcgacg tcggcgagga tggcctcgag
    22861 gcccggcagg ttgccatgca cgtcgctgaa gacggcgaag ggcgtggcga tgcgcagtgt
    22921 cctcaagggg aagtccttta tcgaccgacg tttatatcaa gctaccttga ccataaacgg
    22981 atgtcaatgt aacctgtcgc catggcacag agcaccgtca agcgcctctc gttcctcgac
    23041 cgctacctga cgctgtggat cttcctcgcc atgttcatcg gcgtcggcat cggctgggcg
    23101 ttcgtctccg tgcctgagtt catcgaccgc tggacggtgg gcaccaccaa catccccatc
    23161 gccatcggcc tcatcgtgat gatgtatccg ccgctcgcga aggtgcgcta cgaggagctc
    23221 ggcgacgtct tccgcgacta ccgtatcctc ggcctctcgc tggtgcagaa ctggctcatc
    23281 gggccgttcg tgatgttctt cctcgcgatc atcttcctgc acgactaccc cgagtacatg
    23341 gtcgggctca tcatgatggg ccttgcgcgc tgcatcgcca tggtcatcgt ctggaaccag
    23401 ctggccaagg gcgacaccga gtacgccgcc ggcctggtcg ccttcaacag cgtcttccag
    23461 gtgctcttct acagcgtgtt cgcctgggtc ttcgtcacca agctgccgcc gctcttcggc
    23521 ctcgagggcg tggccgtcga catcaccatc gggcagatcg ccaagagcgt gttcatctac
    23581 ctcggcatcc cgttcctcgc cggcatgctc acccggttcg tcgccctgaa gacgaagggc
    23641 cgcgagtggt acgagcagaa gttcatcccg cgcatcagcc cgctcacgct gatcgccctg
    23701 ctcttcacca tcttcgtgat gttcagcctc aagggcgacc tcatcgtgga gctgccgctg
    23761 gacgtgctgc tcatcgccat cccgctgtcc ctgtacttcg tgatcatgtt cttcgtgagc
    23821 ttcttcatgg ggcgcaagat cggcgccgac tactcaaaga cggcgaccct cagcttcacc
    23881 gcggcgagca acaacttcga gcttgccatc gccgtgagca tcgccgtctt cggcctcaac
    23941 tcgggccagg cgttcgcgag cgtcatcggg ccgctcgtgg aggtcccggt gctcatcagc
    24001 ctcgtgagcg tggcgctctg gctggggcgc aagtactggg gcatgaccga cgaacccgtg
    24061 cctgcggccg tggccgccgg atgtgacgag gtcggctgcg accccgtccg gggaagggag
    24121 acgagctgat gaccgttgag ccggtgcacg gaggcacgat caccgactac gtgccgctgt
    24181 tcaaggcgct ctgcaactcg acccgcgcgc agatcatcga gttcctgctc ggcggcgagc
    24241 gctgcgtgtg cgagatgacc gggccgctcg acgccagcca gc
//
LOCUS       DASUMX010000295         6587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_113739, whole genome shotgun sequence.
ACCESSION   DASUMX010000295 DASUMX010000000
VERSION     DASUMX010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 6587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6587
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113739"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1347)
                     /locus_tag="VFZ86_13985"
     CDS             complement(<1..1347)
                     /locus_tag="VFZ86_13985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX5643427.1"
                     /translation="MTQPESQDRFQVVFEASPFALVVTRMQDGVTVGVNAALERLLGC
                     SRATVIGKASLDLMPGDEAGRALVAAAMRERRSLHGVEFVHRLRTGEERILSVAVTPI
                     RVGGADHVLTTIDDVTEQRRAQDALKDSEARYRSIVETTAEGVIIGRPDGVILYANQQ
                     MADMLGCSVDELVGRSGLDFVFPDWEMKVIENRAALDSGSVLRGEIKLRRKDGSALWT
                     WFSSSAMVDQSGTHVANLTMHTDTTKRRRAEEALRDSEIAKAAQQERNHLARELHDSV
                     TQALFAAALRMEALQRSGEPLSPGVTAALEDVERVNRGALAQMRTMLLELRGEAIEDV
                     PLKLLLRHLAEATQSRARVSVELSTAGEASLPAHVHVATFRIAQEALNNVVRHAHAAT
                     AQVKLELASRGLRLIVADDGCGFDPGCAVDPTHLGLVSMRERATEVGGRLGVTSSPG"
     gene            complement(1370..3658)
                     /locus_tag="VFZ86_13990"
     CDS             complement(1370..3658)
                     /locus_tag="VFZ86_13990"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX5643428.1"
                     /translation="MQEAADLIGVSASTLRRWAAQGRVPCRRTPSGQRRFLVEDLERC
                     LTPEAPSDPGPGSPDTPEEQRYRLLYETSRDLTSSLDLREVLQAAAWRLNTILAIPDC
                     DIYRLVEGERLVCIASTTRGVLDDDWVGREFSLAGWHGTRLAVETHLPIPVGSLDDPR
                     LGAQQREQMLPYGQQSCLTVPLVSRGRAIGVVDLLDYVEREFTAEEIATAEAIGQQVA
                     QALDNAQLYEEVRRLHLGNLRALSSALSAKDYYTLGHASRVAAYTALLGRELGWPEQR
                     LEAVENAAFLHDIGKIGVSDRVLLKAGPLTSEEWELMRQHPGISAEIVRPLFDEELVA
                     GVRHHHERFDGLGYPDGLGGDDIPLTARALCVVDCYDAMSCQRPYRQALSYEQCVAEL
                     ERCSGTQFDPAMVESFRRVLVRLQRRRALVEELAVEAARGIDPAAHALLRTHADQARP
                     EYARMVGALRELRDAHPPVRFISSFSLRGDQCVSVLDTGETEDDLSRCGDPWLPEDGL
                     ARVLAGESLLANVLNADDYGVWVSGIAPVLGAEGEVVAAVTVDAPAVESRGWSAEGDR
                     SQTLAAMLRAATIRFSRAEVEAITDGLTGLYNHRYLHERLEEELERSRRCGGELSVLF
                     CDCDHFKEYNDAHGHKTGDAALGRIARIFEANSRRTDLAARYGGEEFVLALVDTGAAG
                     ARVVAETLRREIEEASGRRGRPLTVSIGVATFPDDAGSRDELLDKADWAMYAAKRAGR
                     NQVLAFSDGLVRNETWLSRRGR"
     gene            3991..5514
                     /locus_tag="VFZ86_13995"
     CDS             3991..5514
                     /locus_tag="VFZ86_13995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013892001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEX5643429.1"
                     /translation="MTHEETPTVDSQVGEESLDPQNWAELRTLAHRAVDDAVDYMETL
                     RERPAWEHAPEHVKAHFDGPPPAGPQPAEEIYAEWLEYVRPYQLGNSHPRFWGWVLGS
                     GTPMGVIAEMLAAATNSVSGIYSYVSNNYVELQVLDWCKQLLGYPLAAGGLLTSGCSA
                     SNLIALAVARNAKAGFDVRGEGLAAAPGVPGEAGASRRLTVYCSAEAHSSLTKAVELL
                     GMGTRSLRRVPTDDLLRIDLEALSEMVARDRAAGLRPICVVGVAGTTSTGSIDELPAL
                     ADFCEREGLWFHVDGAFGAWAAIAPASRHLVAGMERADSLAFDLHKWMNLTYPVGCVL
                     VRDAEAQLQTFSLTPSYLAHGEGERGLTGVDVPWLSDYGYELSRGFNALKAWMALKEH
                     GADKFGRVIQQNIDQARYLAGLVAATPELEQALPVALNIVCFRYVRPGLDDAALDQLN
                     KEIELELQERGIAVPSVSTIHGRKYLHVAVANHRSRREDFDLLVREVVRLGGELATG"
     gene            complement(5634..5957)
                     /locus_tag="VFZ86_14000"
     CDS             complement(5634..5957)
                     /locus_tag="VFZ86_14000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643430.1"
                     /translation="MHHLFLDAWDLLRLNGTRYVRPEVLRRRIAAFDFVTKTTGGGGL
                     EIADLVVTPLGRWVAGMAPRPDLDVVRAKLRRSPKGDWEGAGLVVLPKEDGRGPLRNT
                     RPRPV"
     gene            6079..>6587
                     /locus_tag="VFZ86_14005"
     CDS             6079..>6587
                     /locus_tag="VFZ86_14005"
                     /inference="COORDINATES: protein motif:HMM:NF018021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HEX5643431.1"
                     /translation="MSFESDFHAAARLLAEGDARAAAERLRAVVSAGGLAPGFEAEVR
                     YLLGRALAAGGDRDGMTREWIRVLELDALAVRRPPLLTAEEFESVAQAALDELPQALL
                     DELNGVAIMIEDRPSPELVADGIDPRILGLYHGVPLNRRSASFGAPYADTIHLFRANL
                     ERAFTTRVAL"
BASE COUNT          884 a         2374 c         2352 g          977 t
ORIGIN      
        1 gcccggactg ctggtcacgc cgagccggcc gccgacctcc gtcgcgcgct cacgcatgga
       61 cacgaggccg aggtgggtcg ggtcgacggc gcagccgggg tcgaagccgc agccgtcgtc
      121 ggcgacgatc agccgcaggc cccgggacgc cagctcgagc ttcacctgcg cggtggccgc
      181 gtgggcgtgg cgcaccacgt tgttcagcgc ttcttgcgcg atgcggaagg tcgcgacgtg
      241 cacgtgcgcc ggcaggctcg cctctcccgc cgtcgagagc tctacgctca cccgggcccg
      301 gctctgggtc gcctcggcca ggtggcgcag cagcagcttc aggggtacgt cctcgatcgc
      361 ctctccgcgc agctccagca gcatcgtccg catctgtgcc agggcgccgc ggttgacacg
      421 ctccacgtcc tcgagcgccg cggtcacccc gggagagagt ggttcgccgg agcgctgcag
      481 cgcttccatc ctcagggccg cggcgaagag agcctgcgtg acggagtcgt ggagctcccg
      541 cgccagatgg ttcctctcct gttgcgcggc cttcgcgatc tcgctgtcgc gcagcgcctc
      601 ctcggcacgc ctgcgcttgg tggtgtccgt gtgcatggtc agattggcca cgtgggtgcc
      661 ggactggtcg accatcgccg aggagctgaa ccacgtccac agggcgctgc cgtccttgcg
      721 ccgcagcttg atctccccgc gcagcacgct gccggagtcc agcgcggctc tgttctcgat
      781 caccttcatc tcccagtccg ggaagacgaa gtcgaggccg cttctgccga ccagctcgtc
      841 gaccgagcag ccgagcatgt cggccatctg ctggttcgcg tacaggatga cgccgtccgg
      901 ccggccgatg atgacgccct ccgccgtggt ctcgacgatg ctccggtacc tggcctcgct
      961 gtccttcagc gcgtcctgtg cccgtcgctg ctcggtgacg tcgtcgatgg tcgtcagcac
     1021 gtggtcggcg ccgccgactc ggatcggcgt gacggcgacg gacaggatgc gctcctcacc
     1081 ggtccgcaac ctgtgcacga actcgacgcc gtgcaggctg cgccgctctc tcatcgcggc
     1141 cgcgacgagc gcccggccgg cttcatcgcc cggcatgaga tccaggctgg ccttgccgat
     1201 caccgtcgcg cgggaacatc cgagcagccg ttcgagggcc gcgttcacac cgaccgtgac
     1261 cccgtcctgc atcctcgtca cgacgagggc gaacggcgac gcctcgaaca ccacctggaa
     1321 tctgtcctgg ctctccggct gcgtcatgga cgcattctag ggcagcgcgc tatctcccgc
     1381 gccgtgagag ccacgtctcg ttgcgcacga ggccgtcgga gaaggccagc acctggttgc
     1441 ggccggcgcg cttggccgcg tacatcgccc agtcggcctt gtcgaggagc tcgtcccggg
     1501 agccggcgtc gtcggggaag gtggcgaccc cgatgctgac cgtgaggggg cgcccgcggc
     1561 gcccgctggc ctcctcgatc tcgcggcgga gggtctcggc gacgacgcgc gcgcccgccg
     1621 cgccggtgtc cacgagcgcg agcacgaact cctcgccccc atagcgcgcc gccaggtcgg
     1681 tgcggcggct gttggcctcg aagatgcgcg cgatgcgccc cagggccgcg tcgccggtct
     1741 tgtggccgtg ggcgtcgttg tactccttga agtggtcgca gtcgcagaag agcaccgaga
     1801 gctcgccccc gcagcgacgg gagcgctcga gctcctcctc gaggcgctcg tgcaggtagc
     1861 ggtggttgta gagcccggtg agaccgtcgg tgatcgcctc gacctcggcg cggctgaagc
     1921 ggatcgtcgc cgcgcgcagc atcgccgcca gcgtctgcga gcggtcgccc tcggccgacc
     1981 agccgcgcga ctcgaccgcc ggcgcgtcga cggtgaccgc cgccacgacc tcgccctcgg
     2041 cgcccagcac cggggcgatg cccgagaccc agacgccgta gtcgtcggcg ttgagcacgt
     2101 tggcgaggag gctctcgccg gccagcacgc gcgcgagccc gtcctcgggc agccaggggt
     2161 cgccgcagcg cgagaggtcg tcctcggtct cgccggtgtc cagcaccgag acgcactgat
     2221 cgccgcgcag cgagaagctg ctgatgaagc gcaccggcgg gtgagcgtcg cgcagctcgc
     2281 ggagcgcgcc caccatgcgc gcgtactccg gccgcgcctg gtcggcgtgc gtgcgcagca
     2341 gggcgtgagc cgcgggatcg atgccccgcg cggcctcgac ggcgagctcc tccacgagcg
     2401 ctcgccgccg ctgcaggcgg acgaggacgc ggcggaagct ctcgaccatc gccgggtcga
     2461 actgcgtgcc ggagcagcgc tcgagctcgg cgacgcactg ctcgtagctc agggcctgcc
     2521 ggtagggtcg ctggcaggac atggcgtcgt agcagtcgac gacgcacagg gcgcgggccg
     2581 tcaaggggat gtcgtcgccg ccgaggccgt cggggtagcc gaggccgtcg aagcgctcgt
     2641 ggtggtgccg cacgcccgcc acgagctcct cgtcgaagag cgggcgcacg atctcggcgc
     2701 tgatgccggg gtgctggcgc atgagctccc actcctcgct ggtcagcggg cccgccttga
     2761 gcaggacgcg gtcggagacg ccgatcttgc cgatgtcgtg caggaaggcg gcgttctcca
     2821 ccgcctcgag gcgctgctcg ggccagccca gctcgcgtcc gagcagcgcc gtgtaggcgg
     2881 cgacgcggct ggcgtgaccg agcgtgtagt agtccttggc gctgagggcg gagctgaggg
     2941 cgcgcaggtt gccgaggtgg aggcgccgga cctcctcgta gagctgcgcg ttgtcgagcg
     3001 cctgcgccac ctgctggccg atcgcctcgg cggtggcgat ctcctccgcc gtgaactccc
     3061 gctcgacgta gtcgagcagg tcgaccacgc cgatcgcccg gccgcggctc accagcggca
     3121 ccgtcaggca gctctgctgg ccgtagggca gcatctgctc gcgctgctgc gcgccgagcc
     3181 gcgggtcgtc gaggctcccc accgggatcg ggaggtgcgt ctccacggcg agccgggtac
     3241 cgtgccagcc cgcgagcgag aactccctgc cgacccagtc gtcgtccagg acccctcgtg
     3301 tggtcgaggc gatgcagacc aggcgttcgc cctcgaccag ccggtagatg tcgcagtccg
     3361 ggatcgccag gatggtgttc agacgccacg ccgccgcctg cagcacttcg cgcaggtcga
     3421 ggctggaggt gagatcgcgg ctcgtctcgt agagcagccg gtagcgctgc tcctcggggg
     3481 tgtccgggga tccgggaccg gggtctgacg gcgcctccgg cgtgaggcag cgctcgaggt
     3541 cctcgacgag gaagcggcgc tgcccgctcg gcgtgcgccg gcagggcacc ctgccctggg
     3601 ccgcccagcg acgcagcgtg ctggcgctga ccccgatcag gtcggcagcc tcttgcacgc
     3661 gtacgcagcg cggtctcgtg gtatcggcgc ccacaggtga cgctatcgga ccgccgtgct
     3721 cggaccttga gcacctctgt catgagatcg cgcccagttc accagcgggc agtgcggcga
     3781 cgcggggagg gtatggcgcg cagggtcctc tgaagacgta gagacaatgt agagacattc
     3841 cgccggcttg tgacgcgatc ccgccgagat gtagagccaa tgtctctacg ccaacagagc
     3901 gtccggcgag tgacctcctc ggccgccggc aggcgcgcgg cgccggcgcg atagaatccc
     3961 cgggcccgcc gcgaccgcac gaggaccgcc atgacgcacg aagagacccc gaccgtcgac
     4021 tcccaagttg gggaggagtc cctcgacccc cagaactggg cggagctgcg cacgctcgcg
     4081 caccgcgccg tggacgacgc cgtggactac atggagacgc tgcgcgagcg ccccgcgtgg
     4141 gagcacgcgc cggagcacgt gaaggcgcac ttcgacgggc cgccgccggc ggggccgcag
     4201 cccgcggagg agatctacgc cgagtggctc gagtacgtgc ggccctacca gctgggcaac
     4261 agccaccccc ggttctgggg ctgggtgctg ggcagcggca cgccgatggg ggtgatcgcg
     4321 gagatgctgg ccgcggcgac gaactcggtg agcggcatct actcctacgt gagcaacaac
     4381 tacgtcgagc tgcaggtgct ggactggtgc aaacagctgc tcgggtaccc gctcgcggcc
     4441 ggggggctgc tcacgagcgg gtgttccgcc tcgaacctga tcgcgctggc cgtggcgcgg
     4501 aacgccaaag cggggttcga cgtgcgcggc gaggggctgg cggccgcgcc gggcgtgccg
     4561 ggcgaggccg gcgcgtcgcg acgcctgacg gtgtactgct cggccgaggc gcactcgtcg
     4621 ctgaccaagg ccgtcgagct gctgggcatg ggcacgcgct cgctgcggcg cgtgcccacc
     4681 gacgacctcc tgcgtatcga cctcgaggcc ctctcagaga tggtcgcgcg cgaccgcgcc
     4741 gcagggctgc gccccatctg cgtcgtcggg gtcgccggta cgaccagcac gggctcgatc
     4801 gacgagctgc cggccctcgc cgacttctgc gagcgcgagg gcctctggtt ccacgtggac
     4861 ggcgccttcg gcgcctgggc cgccatcgct cccgcgtcgc ggcacctcgt cgccggcatg
     4921 gagcgcgccg attcgctggc cttcgacctg cacaagtgga tgaacctgac ctaccccgtc
     4981 ggctgcgtgc tggtccgcga cgccgaggcg cagctgcaga ccttctcgct gaccccgtcc
     5041 tacctcgccc acggcgaggg cgagcgcggg ctcaccgggg tggacgtgcc ctggctctcc
     5101 gactacgggt acgagctctc gcgcggcttc aacgcgctca aggcgtggat ggcgctcaag
     5161 gagcacggcg ccgacaagtt cgggcgcgtc atccagcaga acatcgacca ggcgaggtac
     5221 ctcgccggcc ttgtcgcggc cacgcccgag ctcgagcagg cgctgcccgt ggcgctcaac
     5281 atcgtctgct tccgctacgt gcgccccggc ctggacgacg ccgcgctgga ccagctcaac
     5341 aaggagatcg agctggagct gcaggagcgc ggcatcgccg tcccctccgt ctcgaccatc
     5401 catggccgca agtacctcca cgtggccgtg gccaaccacc gcagccgccg cgaggacttc
     5461 gacctgctgg tgcgcgaggt cgtgcgcctg ggcggggagc tggcgaccgg ctgacctcag
     5521 cggtcgaccg cgtggcgccg tctctgcgct gcgctgactc tcgcccgaac atgctgggga
     5581 ctgccgccgc gcgtgcttgg tggcccggcc tttgacagcc cgctgcgcca tctctacact
     5641 ggccttggtc gggtattgcg tagcggtccc cggccatctt ctttgggcag gacgaccagt
     5701 ccggccccct cccaatctcc cttcggactg cggcgcagct tggcgcgcac cacgtcgaga
     5761 tccggtctcg gcgccatgcc cgcgacccag cggccaaggg gtgtgacgac gaggtcggcg
     5821 atctcgagac cgccgccgcc ggtggttttg gtgacgaaat cgaaagccgc gatgcggcgc
     5881 cgtaggacct cgggcctcac gtatcgcgtg ccgttcaggc ggaggagatc ccaagcgtcg
     5941 aggaacaggt ggtgcatgcc gtcatggtag gcggcgccct cgagcccgca cagagtgaca
     6001 ttagcggtcg aagtggctgg acgtgccgcg accgggcctc gcgccgcgag cacggccgcg
     6061 ccggtctatg atgccgtcat gtcctttgag tccgacttcc acgccgccgc gcgcctgctc
     6121 gccgaaggcg acgcgcgcgc ggccgccgag cgcctgcgcg ccgtcgtctc ggcgggcggg
     6181 ctcgcgcccg ggttcgaggc ggaggtgcgc tacctgctcg ggcgggctct cgcggccggc
     6241 ggcgaccgcg acggcatgac gcgcgagtgg atccgggtgc tggagctcga cgccctcgcc
     6301 gtgcggcggc caccgctcct gacggcggag gagttcgagt ccgtcgcaca ggccgccctg
     6361 gacgagctcc cccaggcgtt gctcgacgag ctgaacggcg tcgccatcat gatcgaggac
     6421 cgtccctctc cggagttggt tgccgacggc atcgacccgc gcatcctcgg cctctaccac
     6481 ggcgtgccgc tgaaccggcg ctccgccagc ttcggcgccc cctacgcgga cacgatccac
     6541 ctgttccgcg ccaacctgga gcgcgcgttc acgacgcgcg tcgcgct
//
LOCUS       DASUMX010000296         5114 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_114913, whole genome shotgun sequence.
ACCESSION   DASUMX010000296 DASUMX010000000
VERSION     DASUMX010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 5114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5114
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114913"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="VFZ86_14010"
     CDS             <1..436
                     /locus_tag="VFZ86_14010"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA dimethylallyltransferase"
                     /protein_id="HEX5643432.1"
                     /translation="SLDREGAGRVQTDELWRAGGRFAHRLLALDPGDDREALKERIER
                     RVDEMLAAGALEEVAAARAAGPFSRTAAQAIGVRELCAVLDGELALPEAAARMKARTR
                     ALARRQLTWMRKLPAAARVPASGRPPAAVARHVLGLVRGPSW"
     gene            499..1368
                     /gene="dapF"
                     /locus_tag="VFZ86_14015"
     CDS             499..1368
                     /gene="dapF"
                     /locus_tag="VFZ86_14015"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEX5643433.1"
                     /translation="MQPQWQGLGKVQFSKWQGLGNNYIVLHAEQLPFELTPERVRLLC
                     DRNLGIGSDGILLIGEKTAEARYPLRIFNPDGSEAEMCGNGVRMVARKLKMEGSITGD
                     TVVLDTGAGEIVPKLGDGYEVTVDMGIARFGGEKLEGFDGDCVEQTLNAAGRSFTFTF
                     VDVGNPHAVIQSPWPLELVPLHEIGPVIEEHKYFPRRANVEFVKVLGEHDAKVRVWER
                     GVGETRACGTGATATAVALVRTGQCSSPVTVELPGGKLVVDVRPDWRVFMTGPAEEIY
                     HGDLSLEFLAHLE"
     gene            1402..2565
                     /locus_tag="VFZ86_14020"
     CDS             1402..2565
                     /locus_tag="VFZ86_14020"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HEX5643434.1"
                     /translation="MRIAERVRTLPPYLFAAIERQIAERKAAGIDIISLGIGDPDLPT
                     PPHVVAALAEGAADPATHQYPSNQGELGFREAVAAFYASRFGVTLDPDGQIVPLLGAK
                     EGIAHICQVLLDPGDVALAADPGYPVYVTGPALADGAAVHLPLLPELGFQPDLEAIPA
                     ETARRAKMIFVSYPNNPTGAVIEDDFFARLVAFAQANDVVVVHDNAYADITYDGYVAP
                     SFLATPGAAEVGVEMFSLSKSYNMTGWRAGAVVGDREVVDAYWRLKTNLDSGMFGAVQ
                     RAAAAALGGSQECVHEMCRVYRRRRDLLVEALRTVGMHVEPPKGTIYLWVPVPEGYTS
                     AGFTQQVLEQADVVVTPGAAYGPSGEGYVRLSLTVPDERLEEAVRRIEERVRL"
     gene            2597..3958
                     /gene="hflX"
                     /locus_tag="VFZ86_14025"
     CDS             2597..3958
                     /gene="hflX"
                     /locus_tag="VFZ86_14025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEX5643435.1"
                     /translation="MSGENVVDTGATEARPRSVVFAVVPGGHGDPGEDEALAEIKELL
                     RSADMDWVADVIQHRDTPDRRTYLGKGKMADLKATIAETGATVAVCEDDLSPAQVAAV
                     LDAVDADVLDRTELILTVFSKHAHSLEGTMQVHLAQLEYELTRMRGKGLVMSRLGAGV
                     DMRGPGETKMEVDRRVVRKRIQTLRRRIEQMARTRRTQRARRLRAGVPLIALAGYTNA
                     GKSTLLNALTDAEVSVRDRLFETLDPTSRSYRYRDRDYVLTDTVGFIRKLPHQLVDAF
                     ASTLEETTLADVVLVVADASLEAPEIAVREQTVADVLDRIGSTAPRIVVFNKIDRVDE
                     PKLLRLRALYPEAEFIAAGKGVGLEALQERLGRFFDRALRPVRLLFPYAAAGEMHRLR
                     GIASDVREEHTPEGVVFAARLPVAEAGRYARFRIEPEAPEADDAALDASPEDRSEEAG
                     PDA"
     gene            3951..4406
                     /gene="dut"
                     /locus_tag="VFZ86_14030"
     CDS             3951..4406
                     /gene="dut"
                     /locus_tag="VFZ86_14030"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012299005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEX5643436.1"
                     /translation="MPDAGLPVPVRLLTPAARLPERAYEHDAAWDLYAAEAATLPPLA
                     RAVVGTGIALGLPAHLAALTLPRSGLAARHGVSIVNAPGLIDPGYRGEVRIILLNTDT
                     AATFTVAPGDRIAQLLFLPLTDVTLVPSDVLEETHRGERGFGSSGTGER"
     gene            4409..4870
                     /locus_tag="VFZ86_14035"
     CDS             4409..4870
                     /locus_tag="VFZ86_14035"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX5643437.1"
                     /translation="MDDAAIEGDKPTSRVAGLLVHEGSILMVEQGRGDERYWLLPGGG
                     VQFGETLSDALRRELQEELGLRVGVQRLIAIVESISPEPDYAKHVVHLIFEISAPAEA
                     LPEPQDVKVLNARFLNEFELQSADVRPPVAEFLSSCVREMPSSPQYLGRRW"
BASE COUNT          745 a         1900 c         1768 g          701 t
ORIGIN      
        1 gtcgctcgac cgcgagggcg ccggccgggt ccagacggac gagttgtggc gcgccggcgg
       61 ccgcttcgcg caccggctgc tggccctcga ccccggcgac gaccgagagg cgctcaagga
      121 gcgcatcgag cggcgcgtcg acgagatgct cgccgcgggc gcgctcgagg aggtcgcggc
      181 cgcgcgcgcc gccgggccgt tctcacgcac cgcggcgcag gcgatcggcg tgcgcgagct
      241 ctgcgccgtc ctcgacggcg agctcgcgct gcccgaggcg gcggcgcgca tgaaggcgcg
      301 cacgcgcgcc ctggcgcgcc gccagctcac gtggatgcgc aagctcccgg ccgcggccag
      361 ggtgccggcc tccggccggc cgccggccgc ggtcgcgcgc catgtcctcg gtctggtccg
      421 cggcccgtcg tggtaggatg cgggttccgg cgcggcggct cccgccgcgc gctgtacgct
      481 ctcagggggt catgatccgt gcagcctcag tggcaaggcc tcggcaaggt ccagttctcc
      541 aagtggcagg gtctcggcaa caactacatc gtgctccacg cggagcagct cccgttcgag
      601 ctcacacctg agcgcgtgcg cctcctctgc gaccgcaacc tcggcatcgg ctcggacggc
      661 atcctcctca tcggcgagaa gaccgccgag gcacgttatc cgctgcgcat cttcaacccc
      721 gacggcagcg aggccgagat gtgcggcaac ggcgtgcgca tggtcgcgcg caagctcaag
      781 atggagggct ccatcaccgg cgacacggtc gtcctggaca ccggcgccgg cgagatcgtc
      841 cccaagctcg gagacggcta cgaggtcacc gtggacatgg gcatcgcccg cttcggcggc
      901 gagaagctcg agggcttcga cggcgactgc gtcgagcaga cgctcaacgc cgccggccgc
      961 agcttcacct tcaccttcgt cgacgtcggc aacccgcacg ccgtcatcca gtcgccgtgg
     1021 ccgctcgagc tggtcccgct gcacgagatc gggcccgtga tcgaggagca caagtacttc
     1081 ccgcgcaggg cgaacgtgga gttcgtcaag gtgctcgggg agcacgacgc caaggtccgc
     1141 gtgtgggagc ggggcgtggg ggagacccgc gcctgcggca ccggcgcgac cgccacggcc
     1201 gtcgcgctcg tccggaccgg ccagtgcagc agcccggtca ccgtggagct cccgggcggc
     1261 aagctcgtcg tggacgtgag gccggactgg cgcgtgttca tgaccggtcc cgccgaggag
     1321 atctaccacg gcgacctctc gctcgagttc ctggcacacc tcgagtagga ccgccgccac
     1381 gacccgtact cccggaggac catgaggatc gcagaacgcg tgcgcacgct gccgccgtac
     1441 ctgttcgcgg cgatcgagcg ccagatcgcc gagcgcaagg cggcaggcat cgacatcatc
     1501 agcctcggca tcggcgaccc cgacctgccg acgccgccgc acgtcgtcgc cgcgctcgcc
     1561 gagggcgcgg ccgacccggc gacccaccag tacccgagca atcagggcga gctcggcttc
     1621 cgcgaggccg tggccgcgtt ctacgcgtcg cgcttcggcg tcacgctcga cccggacggc
     1681 cagatcgtcc ccttgctcgg cgccaaggag ggcatcgccc acatctgcca ggtgctgctg
     1741 gacccgggcg acgtcgccct ggccgcggat cccggctacc cggtctacgt caccggccct
     1801 gcgctggcgg acggcgccgc cgtccacctg ccgctgctgc cggagctggg cttccagccc
     1861 gacctggagg cgatcccggc cgagaccgca cggcgcgcca agatgatctt cgtgagctac
     1921 ccgaacaacc cgaccggcgc cgtcatcgag gacgacttct tcgcccgcct cgtggccttc
     1981 gcccaggcga acgacgtcgt cgtcgtccac gacaacgcct acgccgacat cacctacgac
     2041 ggctacgtcg cgccgagctt cctggcgacg ccgggagccg ccgaggtcgg cgtcgagatg
     2101 ttcagcctct ccaagagcta caacatgacc ggctggcgcg ccggcgccgt cgtcggcgac
     2161 cgtgaggtgg tcgacgccta ctggcgcctc aagaccaacc tcgactccgg catgttcggc
     2221 gccgtgcagc gcgccgccgc ggcggcgctc ggcggctccc aggagtgcgt gcacgagatg
     2281 tgccgcgtat accggcggcg gcgcgacctc ctcgtcgagg cgctgcgcac ggtgggcatg
     2341 cacgtagagc cgccgaaggg aacgatctat ctatgggtgc ccgttcccga aggctacacg
     2401 tccgccggct tcacgcagca ggtgctggag caggccgacg tcgtcgtgac gccgggggcg
     2461 gcctacggcc cgtccggcga gggctacgtg cgcctgtccc tgaccgtgcc ggacgagcgc
     2521 ctagaagagg ccgtccggcg gatcgaggag cgcgtacgcc tctgatcgcg accagcggcg
     2581 ccgccgcgga gacgccgtga gcggcgagaa cgtcgtcgac accggcgcga ccgaggctcg
     2641 cccgcgcagc gtcgtcttcg ccgtcgtccc cggcggccac ggcgatcccg gcgaggacga
     2701 ggccctggcc gagatcaagg aactgctgcg ctccgccgac atggactggg tcgccgacgt
     2761 gatccagcac cgcgacaccc ccgaccggcg cacctacctc ggcaagggca agatggccga
     2821 cctcaaggcg accatcgccg agacgggggc cacggtggcc gtctgcgagg acgacctgtc
     2881 gccggcgcag gtggcggccg tgctcgacgc cgtcgatgcc gacgtgctcg accgcaccga
     2941 gctcatcctc acggtgttca gcaagcacgc gcactcgctc gagggcacca tgcaggtgca
     3001 cctcgcccag ctcgagtacg agctcacgcg catgcgcggc aagggcctcg tcatgagccg
     3061 cctgggcgcc ggcgtcgaca tgcgcggtcc gggcgagacc aagatggagg tcgaccgccg
     3121 cgtcgtgcgc aagcgcatcc agacgctgcg ccggcgtatc gagcagatgg cgcgcacccg
     3181 ccgcacgcag cgcgccaggc ggctccgcgc cggcgtgccg ctcatcgccc tggccgggta
     3241 caccaacgcc ggcaaatcga cgctcctgaa cgcgctcacc gacgccgagg tctccgtgcg
     3301 tgaccgcctg ttcgagacgc tcgatccgac ctcacggagt taccgctacc gcgaccgtga
     3361 ctacgtgctc accgacacgg tcggcttcat ccgcaagctg ccgcaccagc tcgtcgacgc
     3421 gttcgccagc acgctcgagg agaccacgct cgccgacgtc gtgctcgtgg tcgccgatgc
     3481 cagcctggag gcccccgaga tcgccgtgcg cgagcagacc gtggccgacg tgctggacag
     3541 gatcggcagc acggcccccc ggatcgtcgt gttcaacaag atcgaccgcg tcgacgagcc
     3601 caagctgctg cgcctgcgcg cgctctaccc ggaggcggag ttcatcgccg ccggcaaggg
     3661 cgtcggcctc gaggcgctcc aggagcgtct cggccgcttc ttcgaccgcg cgctgcggcc
     3721 ggtgcgcctg ctcttcccgt acgccgcggc cggcgagatg caccgtctcc gcggcatcgc
     3781 cagcgacgtg cgcgaggagc acacgcccga gggtgtggtc ttcgccgccc ggctgcccgt
     3841 ggctgaagcc gggcgctacg cgcgcttccg catcgagccc gaggcgcctg aggcggacga
     3901 cgccgccctg gatgcgtcgc ccgaagaccg gagcgaggag gccggccccg atgcctgacg
     3961 ccggccttcc cgtgcccgtg cgcctgctga cgccggcggc ccgcctgccc gagcgtgcct
     4021 acgagcacga cgccgcctgg gacctctacg cggcggaggc ggctacgctc ccgccgctcg
     4081 cgcgcgccgt cgtcggcacg ggcatcgccc tcggcctgcc ggcccatctc gcggcactca
     4141 cgctaccgcg ctccggcctg gccgcgcggc acggcgtttc gatcgtcaac gctcctgggc
     4201 tcatcgaccc gggctatcgc ggcgaggtgc gcatcatcct cctgaacacc gacaccgcgg
     4261 cgaccttcac cgtcgcaccc ggcgatcgca tcgcccagct cctgttcctg ccgctcacgg
     4321 acgttacact ggtgccgtcc gacgttcttg aggagacgca ccgcggcgaa cgcggcttcg
     4381 gctccagcgg gacgggggag aggtgaccat ggacgacgct gcgatcgagg gcgacaaacc
     4441 gactagtcgg gtcgcagggc tgctcgtcca cgagggtagt atcttgatgg tagagcaggg
     4501 gagaggcgac gaacgctact ggctgctccc cggcggcggc gtacagttcg gcgagacgct
     4561 gtccgacgcg ctgcgccgcg agctccagga ggagctcggc ctgcgcgtgg gggtccagcg
     4621 cctgatcgcg atcgtcgagt cgatctcgcc ggagcccgac tatgccaagc acgtcgtgca
     4681 cctgatcttc gagatctcgg cgccggcgga agcgctcccg gagccccagg acgtcaaggt
     4741 gctcaacgcc cgtttcctga atgagttcga gctgcagtcc gccgacgtgc gcccgcccgt
     4801 ggccgagttc ctgagctcgt gcgtgcggga gatgccctcg tcgccgcagt acctcggccg
     4861 tcgctggtag ctcgcgtctg agtcggatgc acggaccggc gcgggcaagc gcgtcgcgcg
     4921 ggccggcgta tctgcgctgg ctctgacgcc acgggtgacg ccacccatga cgccagggcg
     4981 ccaagatatc cccgaagacg cccgagaacg gttgcctcct gcgcagtagt aacacgcgat
     5041 aatcgccgtt atgtaaagct aggtgtggag acgcggcggg cgacctctgg atcaccccgt
     5101 cctcccgtcc gggc
//
LOCUS       DASUMX010000297         4380 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1552787, whole genome shotgun sequence.
ACCESSION   DASUMX010000297 DASUMX010000000
VERSION     DASUMX010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4380
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1552787"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VFZ86_14040"
     CDS             complement(<1..294)
                     /locus_tag="VFZ86_14040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX5643438.1"
                     /translation="MKAVTHSEYGSPDVLELKDVDKPAIKDDEVLVRVRAAAVNPPDW
                     AGVHGVPYIVRLSFGLRRPRLGIRGTDMAGIVEAVGKNVTQLRVGDEVFGRGEG"
     gene            complement(291..728)
                     /locus_tag="VFZ86_14045"
     CDS             complement(291..728)
                     /locus_tag="VFZ86_14045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013683338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6326 family protein"
                     /protein_id="HEX5643439.1"
                     /translation="MNDLGTRISTLWIVVMFNMVFADILTFIKPGALQELWAGQAGVQ
                     ITTGVLLGFAILIEIPIAMIFVSRVLKPRANRWANTVAAVITTAFVVGGGSLTAHYVF
                     FASVEVACMALIVWSVWATRGAEAVAPRATLDRRAGARTEEAS"
     gene            complement(963..1343)
                     /locus_tag="VFZ86_14050"
     CDS             complement(963..1343)
                     /locus_tag="VFZ86_14050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019583465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX5643440.1"
                     /translation="MNVVDSSGWLEYFAGGPNADFFAAAVEAVDELVVPTVSVYEVFK
                     RVLQQRGEGDALQAVALMQQGSVVELTSPIALDAARTSAELGLPMADSIILATARAFQ
                     ATLWTQDSDFEGVHGVRYIASELK"
     gene            complement(1340..1546)
                     /locus_tag="VFZ86_14055"
     CDS             complement(1340..1546)
                     /locus_tag="VFZ86_14055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018878730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEX5643441.1"
                     /translation="MDTVTISPKFQVVIPKRIREKLGLHAGQKVQAIQYGDRVELVPV
                     KPLKQMRGFLRGIDTDVPRETDRL"
     gene            complement(1654..3024)
                     /locus_tag="VFZ86_14060"
     CDS             complement(1654..3024)
                     /locus_tag="VFZ86_14060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HEX5643442.1"
                     /translation="MPAPDQARALEEQIAHWRGYLLRRQAIHSVDVAELEDHLREQVA
                     GLVDAGLTTDEAFLVAVKRMGDLDALSREFAREHSERLWKQLVVVPSDAGEPRTRART
                     DTVVAFGLAVAAAVAIKLPALFGVGLDDEGGFYARNLSLFVLPLLAGYFAWKRRLSTR
                     TILWLALAFAVAAVFANVYPFAPGGYTETLTALHLPLALWLVVGVAYAGSRWRQVAGR
                     MDFIRFSGELLIYYVLIALGGGVLMAFIVLIFQAIAIDVEPFFESWLLPCGATAAVIV
                     AAWLVEAKQSVIENMAPVLTRLFTPFFAAALLVFLGTLLWTGRGIDVERDVLIAFDLL
                     LVVVLGLLLYSVSARDPQAPPNGFDVLQVVLVVSALLADAVALWAIGARITELGFTPN
                     RVAALGENVILLVNLAWSAVLYISFLRGRSSFARLEKWQTDYLPVYAVWAATVVIAFP
                     PLFAYI"
     gene            complement(3028..3411)
                     /locus_tag="VFZ86_14065"
     CDS             complement(3028..3411)
                     /locus_tag="VFZ86_14065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX5643443.1"
                     /translation="MEINKELTAASSTPIVLAILAEGDSYGYAILQRVRELSGGSLQW
                     TDGMLYPVLHRLERLEHVEARWEVSESGRRRKYYRITPRGRAHLAEERRQWQTVDATL
                     RGLWRASPRVPAGHPAPAALLPEGA"
     gene            complement(3492..3953)
                     /locus_tag="VFZ86_14070"
     CDS             complement(3492..3953)
                     /locus_tag="VFZ86_14070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013159022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX5643444.1"
                     /translation="MRTPHRAVRLAAVVLLLVAAVAAAGCSSDETASPAATGAPAETA
                     GYTTVDVQTAHDAVSADQSAQLLDVREPEEWAETGVPDGAVLIPLAEVQSRAPSELDA
                     GSPVYVICRSGNRSRTASETLVQLGFGQVYNVDGGVTAWMDAGLPVQAYQP"
     gene            complement(4021..>4380)
                     /locus_tag="VFZ86_14075"
     CDS             complement(4021..>4380)
                     /locus_tag="VFZ86_14075"
                     /inference="COORDINATES: protein motif:HMM:NF037009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEX5643445.1"
                     /translation="GATLDERIDLIVGSAEQLRQRVERFAAFYELLPEASRDTELRER
                     LMTYYEGWYAWAREVLVPAGADGERRARRAATIGQFASILLDGIFMQMVVAAPGFDLE
                     AALRDARRTLSYLLDRG"
BASE COUNT          704 a         1527 c         1517 g          632 t
ORIGIN      
        1 gccctcgccg cggccgaaga cctcatctcc gacccggagt tgcgtcacgt tcttgccgac
       61 ggcctcgacg atcccggcca tgtccgtgcc gcggatgccg agcctcggcc ggcgcagccc
      121 gaaggacagg cggacgatgt acggcacgcc gtggacgccg gcccagtccg gggggttgac
      181 ggcggcggcg cgcacgcgca cgagcacctc gtcatccttg atggccggct tgtcgacgtc
      241 cttgagctcc aggacgtccg gtgagccgta ctcgctgtgc gtgactgcct tcatgatgct
      301 tcctcggtgc gtgcgccggc acgtcggtcc agcgtggcgc gcggagccac ggcttctgcg
      361 ccgcgcgtcg cccagaccga ccagacgatc agcgccatgc acgcgacctc cacggacgcg
      421 aagaacacgt agtgagcggt cagcgacccg ccgccgacca cgaacgcggt ggtgatcacc
      481 gctgcaacgg tgttcgccca gcggttggcc ctcggcttca ggacgcggga cacgaagatc
      541 atcgcgatcg ggatctctat gagtatcgcg aaccccagca ggacccccgt ggtgatctgc
      601 acgcccgcct gcccggccca cagttcctgc agggctccgg gcttgatgaa ggtcaggatg
      661 tccgcgaaga ccatgttgaa catgaccacg atccagagcg tcgagatcct cgtccccagg
      721 tcgttcatga catcgattcg ccttgagctc ggtatcgtgt cccccatcgt gagtcccacc
      781 ttccccttga gttgagcgcc ttgacccgag gagcctacgg ctgcgccttc cgtcggtcgt
      841 ctcccggcgg gcggagtctg gggtcaactg tgggaggaac ttcggcgcgg gcgccatgcc
      901 cctgcggggg gacgccgccg gcgccggagg ggaggggccc gggcacgcgc tccaacgccc
      961 tgctacttca actcactagc gatgtacctc acgccatgga caccttcgaa gtcggagtct
     1021 tgcgtccaca gcgtggcctg gaaggcgcgc gccgtggcga ggatgatgct gtcggccatc
     1081 gggagaccga gctcggcgct cgtccgtgcg gcgtcaagag cgatgggcga cgtgagctcg
     1141 acgaccgagc cttgctgcat cagcgcgacg gcctgcagcg catcgccctc tccgcgctgc
     1201 tggaggaccc gcttgaagac ctcgtacacg ctgaccgtcg gcacgacgag ttcgtccacc
     1261 gcctcgactg cagcggcgaa gaagtcggcg ttgggccctc cggcgaagta ctcgagccaa
     1321 ccggacgagt cgacgacgtt cacagccggt cggtctcgcg cggcacatcg gtgtcgatgc
     1381 cgcgcagaaa gccgcgcatc tgcttgagcg gcttgacggg aacgagctcg acgcgatcgc
     1441 cgtactggat cgcctggacc ttctggcccg cgtgcaggcc gagcttctca cggatgcgct
     1501 tcgggatgac gacctggaac ttgggcgaga tggtgacggt atccatcaca gtctcctcat
     1561 cgatactgaa aacgtataac gacaagagta acgatgaaga ctacgtaagg caagggtgac
     1621 ggtctggtcg gccgctccgc ccgcgtctct ccgtcagatg taggcgaaca gcggcgggaa
     1681 ggcgatcacg acggtcgccg cccacacggc gtagaccggc aggtagtcgg tctgccactt
     1741 ctcgagcctc gcgaaggagc tgcgccctct caagaagctg atgtagagca cggcggacca
     1801 cgccagattg accaggagga tcacgttctc cccgagggcg gccacgcggt tcggcgtgaa
     1861 gccgagctcg gtgatgcgcg cgccgatcgc ccacagcgcc accgcgtcgg ccaggagcgc
     1921 gctgaccacc agcacgacct gcagcacgtc gaagccgttg ggcggcgcct gggggtcgcg
     1981 ggcggagact gagtagagca gcaggccgag caccaccacc aggagcaggt cgaaggcgat
     2041 gaggacgtcc cgctcgacgt cgatgccgcg tccggtccac agcagcgtcc ccaggaacac
     2101 gagcagcgcg gcggcgaaga agggcgtgaa gaggcgcgtc agcaccggcg ccatgttctc
     2161 gatgacgctc tgcttggcct ccaccagcca ggccgcgacg atgaccgccg cggtcgctcc
     2221 gcacggcaac agccacgact cgaagaacgg ctcgacgtcg atcgcgatcg cctggaagat
     2281 cagcacgatg aaggccatga ggacgccgcc gccgagcgcg atcaggacgt agtagatgag
     2341 cagctccccc gagaagcgga tgaagtccat gcggccggcg acctgtctcc agcggctgcc
     2401 cgcgtaggcg acgccgacca ccagccacag cgccagcggc aggtgcagcg ccgtgagcgt
     2461 ctcggtgtag ccacccgggg cgaacggata gacgttggcg aagacggccg ccacggcgaa
     2521 cgccagggcc agccagagga tggtgcgggt ggagagccgc cgcttccagg cgaagtaacc
     2581 ggccagcagg ggcagcacga agaggctcag gttgcgggcg tagaagccgc cctcgtcgtc
     2641 caggccgacc ccgaagagcg ccggcagctt gatggcgacg gccgccgcca ccgcgaggcc
     2701 gaaggcgacg acggtgtccg tgcgcgcccg tgtccgcggc tccccggcgt ccgaagggac
     2761 cacgacgagc tgcttccaga ggcgctccga gtgctcgcgc gcgaactcgc gcgagagcgc
     2821 gtcgaggtcg cccatgcgct tcaccgccac caggaacgcc tcgtccgtgg tgagccccgc
     2881 gtccacaagg ccagcgacct gctcgcgcag gtggtcctcc agctccgcca cgtcgacgga
     2941 gtggatcgcc tgccggcgga ggaggtagcc ccgccagtgg gcgatctgct cctccagtgc
     3001 tcgcgcctga tccggggccg gcatcgctca ggccccctcc ggcaggagcg ctgccggcgc
     3061 cgggtggccg gccgggacgc gcggggacgc gcgccagagt ccccgcagcg tcgcgtccac
     3121 cgtctgccac tggcggcgtt cctcggcgag gtgggcccgg cctcggggcg tgatccggta
     3181 gtacttgcgc cggcggccgc tctccgagac ctcccaccgc gcctcgacgt gctcgagccg
     3241 ctcgaggcgg tgcaggacgg ggtagagcat cccgtccgtc cactgcaggc ttcctccgga
     3301 gagctcacgg acccgctgga ggatggcgta gccgtagctg tccccctcgg ccaggatcgc
     3361 caggacgatg ggggtggagg acgccgccgt gagttccttg ttgatctcca tagccagcgg
     3421 cagtatgcat agcagcgcta ggtatgtcaa agccgcggct gggagtcgcg cctccggccg
     3481 cggcgcgggg tctacggctg gtaggcctgc acgggcaatc cggcgtccat ccaggcggtg
     3541 acgccgccgt ccacgttgta gacctgcccg aagcccagct ggaccagcgt ctccgaggcg
     3601 gtgcggctgc ggttgccgct ccggcagatc acgtagacgg gactgccggc gtccagctcg
     3661 gacggcgcgc gcgactggac ttcggcgagc gggatgagca ccgccccgtc ggggacgccc
     3721 gtctcggccc actcctccgg ttcgcgcacg tcaagcagct gggcgctctg atctgcgctc
     3781 accgcgtcgt gggcggtctg gacgtccacc gtcgtgtagc cggcggtctc cgcgggcgcg
     3841 ccggtcgcgg ccggagacgc cgtctcatcg ctcgaacagc cggctgcggc caccgccgcc
     3901 accagcagca gcacgacggc cgccagtctt actgcacgat gtggtgttcg catggtcggg
     3961 actcctcggt cgcgcgtggg cgtgcatcgg caactgtacc cgcaagcgcg gcatccggag
     4021 tcatccacgg tcgagcaggt agctcaacgt ccggcgcgcg tcccgcagcg ccgcctcgag
     4081 gtcgaagccc ggcgcggcga cgaccatctg catgaagatc ccgtcgagca ggatcgaggc
     4141 gaactgcccg atcgtcgccg ccctccgggc gcgccgctca ccgtccgcgc cggcgggcac
     4201 gagcacttcg cgggcccagg cgtaccagcc ctcgtagtag gtcatgaggc gctcgcgcag
     4261 ctccgtgtcg cgggaggcct cgggcaggag ctcgtagaag gcggcgaagc gctccacccg
     4321 ctgcctcagc tgctccgcgc tgcccacgat gaggtcgatg cgctcgtcga gcgtggcgcc
//
LOCUS       DASUMX010000298         4780 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_977816, whole genome shotgun sequence.
ACCESSION   DASUMX010000298 DASUMX010000000
VERSION     DASUMX010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4780
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_977816"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..172)
                     /locus_tag="VFZ86_14080"
     CDS             complement(<1..172)
                     /locus_tag="VFZ86_14080"
                     /inference="COORDINATES: protein motif:HMM:NF026171.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEX5643446.1"
                     /translation="MIRYLDTRGAIDRPRTFTEAILEGIAPGGGLFVPERLPRFTVEQ
                     IVALADVPYHARA"
     gene            318..980
                     /locus_tag="VFZ86_14085"
     CDS             318..980
                     /locus_tag="VFZ86_14085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011027749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX5643447.1"
                     /translation="MYGKQYAGGGEGRPQTQEGRGRHGHGGGRGSDCGPARFRSGGGE
                     GGRGSGRGQGGRSGGHGHGHGFGRGPWSRARRGDVRAAILLALVDEPMHGYQIMQRLE
                     ERSGGAWRPSPGSVYPTLQLLEDQGLIKGEEAEGRRVFSLTEAGAAEAAVVKERLGDA
                     PFGAEGAEQDPRYALRQAVFQLGAAVKQVGMAGSAADVQKALEILREARKRMYALLAD
                     AE"
     gene            complement(1125..2987)
                     /locus_tag="VFZ86_14090"
     CDS             complement(1125..2987)
                     /locus_tag="VFZ86_14090"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5643448.1"
                     /translation="MRPEFRADLHVHSRYSDTSGTAGVRALRSAESFTDPVELYHAPR
                     ARGMDFVTVTDHNTLAGSLAIAHLPGTFLSCEFDSWFPEDQVRVHVVALGIDERAFAA
                     ANAARGNVHELVACLREADVTHYLAHPLFDMSGKLTPESVERMLLLFNVLEGRNGART
                     SRCNGLLRAIAEALTREQIEAMAERQGIEPYGETPWRKALTGGSDDHSGLFSASAHTV
                     AGGDGTAEGFLRAVAVGDCRHAGADGDARLLAHSIYAASFWKIREILRLDDRQEQRRA
                     LGLLRKGFGRIGRDVPVLEKTLRGVRSIAPGLYRDGDPRGPAWEALLEEEIGSLIAHP
                     DGINAVDAKELNRRLFVVAQRLADEVMGMHLRALLDRGSRMSLKQRLQSIYAVGMVAF
                     LEIPYYFAWSFQSRDRELQEQLRAYFLGERRHTRREKVAVLCAAPPRAARPGHDGAPE
                     ERHGAGAPGAPPRHDVDVTLLTCSVATDVAPAGAVDFRALAWRPSPNGEAPGWVVPPL
                     VEIIDYLEEEEFSALHTDSAAAQGLVTLAAARLLHLPVTGAVDPGGLRAPDGPGDLAG
                     RLRLRYRSWFYGRLDEAFVPTPASARALVAAGVEPSRVTLLPVAPGDDVTADRG"
     gene            3099..3632
                     /locus_tag="VFZ86_14095"
     CDS             3099..3632
                     /locus_tag="VFZ86_14095"
                     /inference="COORDINATES: protein motif:HMM:NF025116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX5643449.1"
                     /translation="METTHHAVLTAVGDDRPGLVDEVTRFITERGGNLEDSRMVNLRG
                     QFAMMMLVAGSESAMSRLQDGLGSLRRGSHVHAELRPADAGAAERPPALPFRLNTWAM
                     DHPGLMQSVSHLLGQLGVNIESVDSSLRPAPYTNAPLFEMELVISVPASTHVAGLREA
                     LGELCDEMNIDWQLTAL"
     gene            complement(3670..>4780)
                     /locus_tag="VFZ86_14100"
     CDS             complement(3670..>4780)
                     /locus_tag="VFZ86_14100"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HEX5643450.1"
                     /translation="AAAPEAADCDFVVGRLRELEEVVRLGLPLPPGKLPNDLMAGFLA
                     GAAGDDPAVVVPPRVGEDVAALDLAGAELLVAHGDPITLGGEDVGRAAVLVNVNDIAA
                     AGAEPRWLLATVLLPVGTTPSQALAQLRGLEAAVAAAEGTLVGGHTEVTDAVARPVVS
                     LTALGTVPRAGLKDKRSARAGDRILLTKELAMEGAALLAGELGERLRALGMSAAELDA
                     CRALQERASILPEARIARGFTGVHAMHDVTEGGLAAALRELASATGRGVLVHRDRIPV
                     SAGTRRICDLLGADPLGLIASGSLLVCCDPEEAPALLAALAGAGIAAAEIGELTEDGA
                     AVVALERDLPAPWPDFATDEAARLLASRAGGTGEG"
BASE COUNT          633 a         1751 c         1793 g          603 t
ORIGIN      
        1 ccgcgcgcgc gtggtacggc acatccgcca gcgccacgat ctgctccacc gtgaagcgtg
       61 gcagccgttc ggggacgaac aggccgccgc ccggggcgat gccctcgagg atcgcctccg
      121 tgaacgtgcg cggccggtcg atggcgccgc gtgtgtcgag gtagcggatc atggccacgg
      181 gattctacag gaagcgagag gcctgaaggc cgccgccggg gtcgccgcga cgtccgtggt
      241 ttgcgggctt gacttctggg aacgatgcat tacgatatat cgcgttagag cgataccgag
      301 cgtccaagga ggacgacatg tacggaaagc agtatgcggg gggcggcgag ggccgccctc
      361 agacacaaga aggtcgcggc cgtcacggcc acggcggcgg acgcggaagc gactgcgggc
      421 cggcgcgctt ccgctccggc ggcggcgagg gagggcgcgg cagcggccgc ggtcagggcg
      481 gtcgctccgg cggccacggc cacggccacg gtttcggccg cggtccctgg tcgcgcgcca
      541 ggcgcggcga cgtgcgcgcg gcgatcctgc tcgcgctcgt cgacgagccg atgcacggct
      601 accagatcat gcagcggctc gaggagcgca gcggcggcgc ctggcggccc agccccggct
      661 cggtctaccc gacgctccag ttgctcgaag accagggcct catcaagggc gaggaggccg
      721 agggcaggcg cgtcttctcc ctgaccgagg ccggcgcggc ggaggccgcc gtcgtcaagg
      781 agcgcctggg cgacgcgccc ttcggcgccg agggcgccga gcaggacccg cgctacgcgc
      841 tgcgccaggc ggtgttccag ctcggcgcgg ccgtcaagca ggtcggcatg gccggctcgg
      901 cggccgacgt gcagaaggcg ctggagatcc tgcgcgaggc ccgcaagcgc atgtacgcgc
      961 tcctcgcgga cgcggaatga ggaaggaggc gccatgaagt ccaccgaggc ggacgaccag
     1021 atcacccggg acgaccaccg tccgtggccc gaaggccaca cggccggact actgcacgga
     1081 agcgctagtg agcggccgtc ccccgaaggg ccacacggcc ggactcagcc ccggtcggcc
     1141 gtgacgtcgt ctcccggagc caccggcagc agcgtgacgc gggacggctc cacgcccgcc
     1201 gcgacgagcg cgcgagcgga ggcgggcgtc ggcacgaacg cctcgtccag gcggccgtag
     1261 aaccaggagc ggtagcggag ccggagccgc ccggcgaggt cgccgggccc gtccggcgcc
     1321 ctgagcccgc ccgggtccac ggcgccggtg acgggcaggt gcagcagccg ggccgcggcg
     1381 agggtcacga ggccttgcgc cgccgccgag tcggtgtgca gggcgctgaa ctcctcctcc
     1441 tcgaggtagt cgatgatctc cacgagcgga ggcaccaccc agcccggcgc ttcgccgttc
     1501 ggcgacggcc tccaggccag cgcgcggaag tccacggcgc cggccggcgc gacgtcggtg
     1561 gcgaccgaac aggtgagcag cgtgacgtcc acgtcgtgcc gcggcggggc gccgggcgcc
     1621 cccgcgccgt gtctctcctc cggggcgccg tcgtggccgg gccgcgccgc ccgcggcggc
     1681 gccgcgcaga gtaccgcgac cttctcccgg cgcgtgtgcc ggcgctcacc caggaagtag
     1741 gcgcgcagct gctcctgcag ctcccggtcg cggctctgga agctccaggc gaagtagtag
     1801 gggatctcga ggaaggccac catgcccacc gcgtagatgc tctggagccg ctgcttgagg
     1861 ctcatccggg agccccggtc gaggagcgct cgcaggtgca tgcccatgac ctcgtcggcg
     1921 aggcgctggg cgaccacgaa gagccgccgg ttgagctcct tggcgtcgac ggcgttgatg
     1981 ccgtcggggt gggcgatgag gctgccgatc tcctcctcga ggagcgcttc ccaggctggg
     2041 cctcgggggt cgccgtcccg gtacagcccc ggggcgatgc tgcgcacgcc gcggagcgtc
     2101 ttctcgagca ccgggacgtc gcggccgatg cgcccgaagc ccttgcgcag caggccgagg
     2161 gcgcgcctct gctcctgccg gtcgtccagg cgcaggatct cccggatctt ccagaaggag
     2221 gctgcgtaga tgctgtgcgc cagcaggcgc gcgtcgccgt cggcgcccgc gtggcggcag
     2281 tcgccgacgg cgacggcgcg cagaaagccc tccgctgtgc cgtcgccccc cgctacggtg
     2341 tgcgcgctcg ccgagaagag gccgctgtgg tcgtcggaac cgcccgtcag cgccttgcgc
     2401 cagggcgtct cgccgtaggg ctcgatgccc tgacgctccg ccatcgcctc gatctgctcg
     2461 cgcgtgagcg cctctgctat tgcgcgcagg aggccgttgc agcggctcgt gcgcgccccg
     2521 ttgcggcctt cgagcacgtt gaacagcagg agcatgcgct cgaccgattc cggcgtgagc
     2581 ttgccgctca tgtcgaagag cgggtgcgcg aggtagtgcg tgacgtccgc ctcgcgcagg
     2641 cacgcgacca gctcgtgcac attgccgcgt gccgcgttcg ccgccgcgaa ggcccgctcg
     2701 tcgatgccga gcgccaccac gtgcacgcgg acctggtcct cggggaacca gctgtcgaac
     2761 tcgcagctca ggaacgtgcc cggcaggtgc gcgatcgcca gcgagcccgc gagcgtgttg
     2821 tggtcggtga cggtgacgaa gtccatgccc cgcgcgcgcg gggcgtggta gagctcgacg
     2881 gggtcggtga acgactcggc gctgcgcagc gcccggacgc ccgcggtgcc cgaggtgtcg
     2941 gaatacctgg agtggacgtg gagatcggct ctgaactctg gtctcacgcc ttcactctac
     3001 cgcaaggcgc gcggcctaga cgggtgaccg cccccccggg cgctccacca cgggccgggg
     3061 ctttgtcttt ctgccgcgat gccgctactg tctctggcat ggagactact caccacgccg
     3121 tgctcaccgc cgtcggggac gaccgccccg gcctcgtcga cgaggtcacc cgcttcatca
     3181 ccgagcgcgg cggcaatctc gaggacagcc gcatggtcaa tctgcgcggc cagttcgcga
     3241 tgatgatgct ggtcgccggc agcgagagcg ccatgagccg gctccaggac ggcctcgggt
     3301 cgctgcggcg cggcagccac gtccacgccg agctgcggcc ggcggacgcc ggcgcggcgg
     3361 agcggccccc ggcgctgccc ttccggctca acacctgggc catggaccac cccggcctca
     3421 tgcagtcggt gtcgcacctc ctcgggcagc tgggcgtgaa catcgagagc gtggactcct
     3481 cgctgcgccc ggcgccgtac acgaacgcgc cgctgttcga gatggagctc gtgatctcgg
     3541 tgccggcgag cacgcacgtc gccggcctgc gcgaggcgct cggggagctc tgcgacgaga
     3601 tgaacatcga ctggcagctc acggccctct gagggccggc cccttcccgt ctcggccgcc
     3661 cgcgcggcct cagccctctc ctgtccctcc ggccctgctc gccagcaggc gcgcggcctc
     3721 gtcggtcgcg aagtccggcc acggcgccgg caggtcgcgc tccagcgcca ccaccgcggc
     3781 gccgtcctcg gtgagctcgc cgatctctgc ggcggcgatg ccggcgccgg ccagcgcggc
     3841 gagcagagcg ggcgcctcct ccggatcgca gcagacgagc aggctgccgg aggcgatgag
     3901 gcccagcggg tcggcgccca gcaggtcgca gatgcgtcgc gtcccggccg agacggggat
     3961 gcggtcgcgg tgcacgagga cgcccctccc ggtcgccgag gccagctcgc gcagcgccgc
     4021 ggcgagccca ccctccgtga cgtcgtgcat cgcgtggacg ccggtgaagc cgcgggcgat
     4081 gcgcgcctcc ggcaggatgc tcgcccgctc ctgaagggcg cggcaggcgt ccagctccgc
     4141 cgcgctcatg cccagggcgc ggaggcgctc gccgagctcc ccggcgagca gcgccgcgcc
     4201 ctccatcgcc agctctttgg tgagcaggat gcggtcgccg gcgcgcgcgg agcgcttgtc
     4261 cttgagcccg gcgcgaggca cggtgccgag cgccgtgagc gacacgaccg gcctggcgac
     4321 ggcgtccgtg acctccgtgt ggccgccgac cagcgtcccc tccgcggcgg ccaccgccgc
     4381 ctcgagtccg cgcagctgcg ccagcgcctg cgacggcgtg gtgcccacgg gcagcagcac
     4441 cgtcgccagc agccagcgcg gctcggcgcc ggccgccgcg atgtcgttga cgttgacgag
     4501 gacggcggcg cggcccacgt cctcgccgcc gagcgtgatg ggatcgccgt gggccacgag
     4561 cagctcggcg ccggccaggt cgagcgcggc gacgtcctcc ccgacgcggg gaggcacgac
     4621 gaccgcgggg tcgtcgccgg cggcgccggc gaggaacccg gccatgaggt cgttgggcag
     4681 cttgccgggc ggcagcggca ggccgaggcg cacgacctcc tcgagctccc ggaggcggcc
     4741 gacgacgaag tcgcagtcgg ccgcctcggg cgcggcggcc
//
LOCUS       DASUMX010000299         6669 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1985508, whole genome shotgun sequence.
ACCESSION   DASUMX010000299 DASUMX010000000
VERSION     DASUMX010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 6669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6669
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1985508"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            526..729
                     /locus_tag="VFZ86_14105"
     CDS             526..729
                     /locus_tag="VFZ86_14105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2180 family protein"
                     /protein_id="HEX5643451.1"
                     /translation="MQCYVCAQAGKQTEAVGICIVCGMGLCMDHALREDVDFWEGGYP
                     FPNKKSRKKLPRILCAECKEVVG"
     gene            790..2322
                     /locus_tag="VFZ86_14110"
     CDS             790..2322
                     /locus_tag="VFZ86_14110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2193 domain-containing protein"
                     /protein_id="HEX5643452.1"
                     /translation="MSLYEKMINEAVGATKAVFGVNKANRGGTFKVTDAKPYVDAVNQ
                     MTVYEGQSKEVIDLHVKSVNAHFDVMTSLTETVRPEDDPFVEHYQTPPILEVMYEEDP
                     EFKASMWKFIDAIAANKALIGRESVRRYGGMYGPTCVVDFAMSVGSVPNVVNQILAPM
                     DIPVAHKQTILACKSWGMNTSYGLAGAFRGAVESGKTMAEAEQAEVEMLQFVYREPSA
                     AQAQLMDTHNLGGHGPHTSFDTRKYMAGYKEKMRPTIVAAMKAGVHYANITAVPAYCV
                     GDVAHHIAQSAYNMFNDDMVFAIYEAVTGVLENTLDRALKADAFKGPYDVLAVATGST
                     ACATAYILWKDSFTVPMVIDLLNKRFHNYAAMHPDRGEADELHNVDFMDILKRGEAIL
                     DITPLGKGGKIKGVEVDLSTVDSNEVISNPQRYTYPACAITQRFAALMTLSDFPCFLT
                     PECTTATLMTNAIAVAPGTPGAPVRGCKQCATCDLIKRNVPMATGIGSGAKGYCQWHL
                     TV"
     gene            2441..3175
                     /locus_tag="VFZ86_14115"
     CDS             2441..3175
                     /locus_tag="VFZ86_14115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014406754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HEX5643453.1"
                     /translation="MPQPTLAKRALAELIGTWVLVFLGTGSVMAMAVNFSEAGGVNQA
                     ALLLIGISFGVAVLVMIYAFGHISGTHINPAVSIALWLTGRFPTKDMVTYVIVQLVGA
                     FLATATVYACWGSRAVDAGLGITLPGAEVSYIQAILVEIVLTFFLGLAIWGAAVDGRA
                     PAGFAGLAIGLVVAADIWVGGPLTGASMNPARSFGPFLGDTLFGGSAADWAKFPIYLI
                     GPIVGLALAAFVYDYISDLKKKGAAE"
     gene            3318..3956
                     /locus_tag="VFZ86_14120"
     CDS             3318..3956
                     /locus_tag="VFZ86_14120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013037958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HEX5643454.1"
                     /translation="MNDKGITLLGISGSSRLKATHFAVNEALRYAHERYGVTVDSFSA
                     HRKTIGFCVHCDACVRKKQGCAFKDDMEDLYPRLVRADAWILGTPVYQGQVSAQLKAM
                     MDRTRAVVAGDPQVFRGKVGAAIAVGGDRSGGQEPTLLAIAGFYLINEMIPVGGGAFG
                     ANLGAAVWSRDKGADGARDDEEGMRAVRHVVDRLIDVTRTMRAGEDARRSRG"
     gene            3953..4519
                     /gene="afpA"
                     /locus_tag="VFZ86_14125"
     CDS             3953..4519
                     /gene="afpA"
                     /locus_tag="VFZ86_14125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archaeoflavoprotein AfpA"
                     /protein_id="HEX5643455.1"
                     /translation="MTLKVVWGITGSGDKMPETVAAMEDLRGRLDVEITAAVSVAGEV
                     VLRWYKLRETVDELAKSVKIEKHANSPFITGPLQIGHYDCLLVAPATANSVAKIVHGI
                     EDTMLTNAVGQTAKSTTPIYIMPVDLHPGTTVTMRPGGERLELKIREIDVANSNRLRT
                     MEGITVFESPSEIEGILRACAERRDAAP"
     gene            4516..5484
                     /locus_tag="VFZ86_14130"
     CDS             4516..5484
                     /locus_tag="VFZ86_14130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ribofuranosylaminobenzene 5'-phosphate
                     synthase family protein"
                     /protein_id="HEX5643456.1"
                     /translation="MRVSVETGCRLHLGFTNLSEDIGRCFGSIGVAIDRPGTSIVFDE
                     HDELAIAGEDREPVRAAVRRFCDTYGVAPRVSITVKEHIPEHVGLGSGTQLALAVGVG
                     LAAVCGIDADVREIASALQRGRRSGIGIAAFRAGGVVIDAGSRRQGADRAGVPTVVWR
                     HDFPADWCFVIAVPAGREGLSSHGEEGVFASLTPSVHISEEVCRITQLLLMPALVERD
                     IEAFGRALTAIDRKTGAYFSDVQGGLYSHEATNETVAAMLKEGAFGAGQSSWGPAVYG
                     LVRAEQAARLESRMREHLRQSGLGDLVTIAHGRNTPAKVEVRSDAL"
     gene            complement(5479..6066)
                     /locus_tag="VFZ86_14135"
     CDS             complement(5479..6066)
                     /locus_tag="VFZ86_14135"
                     /inference="COORDINATES: protein motif:HMM:NF016199.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF447 domain-containing protein"
                     /protein_id="HEX5643457.1"
                     /translation="MSPLRELALTPGWIYEAVVCTDLEGSPHASPAGVWTTDLRSLNM
                     ELYDTSRSLPALLAAGEFCVDLPADAGAFFLALYDPSALSFGRASVVSAPCLTDATAT
                     VELAVAEATPHDGRTLVTGAVRHVTTRGGVRLINRAEGLLVESLVAATRAGLRSREST
                     LALVTENARVAAKVAPGSRFATTLARLRDGLAQES"
     gene            6188..>6669
                     /locus_tag="VFZ86_14140"
     CDS             6188..>6669
                     /locus_tag="VFZ86_14140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent
                     methylenetetrahydromethanopterin dehydrogenase"
                     /protein_id="HEX5643458.1"
                     /translation="MKKILIQFDSDRVAAVFDAVTAYDAGIDALLQYGGIEPGEVRDL
                     VYGAMFTRGPADLKNTAVFIGGIDVAKGEAILREAVGSFFDPLRVSVMLDSNGCNTTA
                     AAAVVKVISTVPVDGQKVVVLAGTGPVGQRAAGLLAAEGAQVTLTSRTLERAQTACAA
                     V"
BASE COUNT         1104 a         2285 c         2266 g         1014 t
ORIGIN      
        1 agccgctctg cggccccggc gaggccatcg ccctggtcac cgacgccggc ctcgagcacg
       61 cgcaccgctt cggcctcgag gacgccgccg ccctggcgcg cttcctcgcc gtgcgcctgg
      121 acgcgctgcg ggtgtactga gcggacaaga ccctctccgg cgattgcccg attcatccat
      181 acctattgcc cttgacagcc gactaacccg ttcatagagt gagccggcgc cacctgagag
      241 gctacccccg cggaagggat ggtcccgctg tcatcgtcgc cgcgcgcacg acagccgggc
      301 tgccgagaac gttcggcagc ccggctgtcg tgcgttcggt cagggatcca gacagagcag
      361 cgcaaaggag gtgaggcgta ctcccagggc cttccgcacg cgtcgtctca gtcccgtcgt
      421 cgtcaccccg gggcactgcg gaaaactaac ggcgacttct cgtcgctctc atgctagaca
      481 gtaatcctca ccacgtcgtt ctcaccaacg cgaaggaggt caggtatgca gtgttatgtg
      541 tgcgcgcagg ccggcaaaca gaccgaagcg gtcggcatct gcatcgtgtg cggcatgggg
      601 ctctgcatgg atcacgcgct cagggaggat gtcgacttct gggagggcgg ctacccgttc
      661 cccaacaaga agtcccggaa gaagctgccg cgcatcctct gtgcggagtg caaggaagtg
      721 gtcggctgag gtagaccggc gacggtcgat ccccagaagg taaacggtca aacgagcacg
      781 gaggtagaga tgtcgctgta cgagaagatg atcaacgagg ccgtgggcgc cacgaaggct
      841 gtcttcggcg tcaacaaggc caaccgcggc ggtaccttca aggtcacgga cgccaagccg
      901 tacgtcgacg ccgtcaacca gatgacggtg tatgaggggc agtccaagga ggtcatcgac
      961 ctccacgtga agtccgtcaa cgcgcacttc gacgtgatga cctcgctcac cgagacggtg
     1021 cgtcccgagg acgacccgtt cgtcgagcac taccagacgc cgccgatcct cgaggtcatg
     1081 tacgaggagg atcccgagtt caaggcgtcc atgtggaagt tcatcgacgc catcgccgcc
     1141 aacaaggccc tcatcggccg tgagtccgtg cgccgctacg gcggcatgta cggccccacc
     1201 tgcgtcgtcg acttcgcgat gtcggtcggg tcggtgccga acgtggtcaa ccagatcctg
     1261 gcgccgatgg acatcccggt cgcccacaag cagaccatcc tcgcctgcaa gtcgtggggc
     1321 atgaacacct cgtacggcct cgccggcgcg ttccgcggcg ccgtcgagtc gggcaagacc
     1381 atggccgagg ccgagcaggc cgaggtcgag atgctgcagt tcgtgtaccg cgagccctcg
     1441 gccgcccagg cgcagctcat ggacacgcac aacctcggcg gccacggccc gcacacctcc
     1501 ttcgacacgc gcaagtacat ggcgggctac aaggagaaga tgcgcccgac gatcgtcgcc
     1561 gccatgaagg ccggcgtgca ctacgccaac atcacggccg tgcccgccta ctgcgtcggc
     1621 gacgtcgccc accacatcgc gcagtcggcc tacaacatgt tcaacgacga catggtgttc
     1681 gcgatctacg aggcggtcac cggcgtactc gagaacacgc tggaccgcgc cctgaaggcc
     1741 gacgccttca agggccccta cgacgtcctg gcggtggcaa cgggctccac tgcgtgcgcg
     1801 acggcctaca tcctgtggaa ggacagcttc accgtcccga tggttatcga cctgctcaac
     1861 aagcgcttcc acaactacgc cgccatgcat ccggatcgcg gcgaggccga cgagctgcac
     1921 aacgtcgact tcatggacat cctcaagcgc ggtgaggcga tcctcgacat caccccgctc
     1981 ggcaagggtg gcaagatcaa gggcgtcgag gtcgacttga gcacggtcga cagcaacgag
     2041 gtcatcagca acccgcagcg ctacacctac ccggcctgcg ccatcacgca gcgcttcgcc
     2101 gccctgatga cgttgtccga tttcccgtgc ttcctcacgc ctgagtgcac caccgctacc
     2161 ctcatgacga acgccatcgc cgtggccccc ggcacgccgg gcgctccagt gcgtggctgc
     2221 aagcagtgcg ccacctgcga cctgatcaag cggaacgtgc cgatggcgac cggtatcggc
     2281 tccggcgcca agggctactg ccagtggcac ctgaccgtct gagcgggtcg atcgccggcc
     2341 cgatctgacg tcgcctttcg aggcggacgg ggaccggggt gctcgcaccc cggtccccgt
     2401 caccagacct gttcagcaac tcgatcagtg gaggtgaacc gtgccgcaac ccaccctcgc
     2461 gaagcgtgcg cttgcggagc tgatcggcac ctgggtgctc gtcttcctcg gtaccggctc
     2521 cgtcatggcc atggccgtga acttctccga agcgggaggc gtcaaccagg ccgctctcct
     2581 gctcatcggc atatcgttcg gtgtggcggt cctcgtcatg atctacgcct tcgggcatat
     2641 ctcggggacc cacatcaacc cggcggtcag catcgcgctg tggctcaccg ggcgcttccc
     2701 gaccaaggac atggtgacgt acgtcatcgt gcagttggtc ggcgcgttcc tcgctacggc
     2761 gacggtctac gcatgctggg ggtcgcgcgc cgtcgacgcc ggactcggca tcacgctgcc
     2821 tggagccgag gtgagctaca tccaggcgat cctcgtggag atcgtcctga cgttcttcct
     2881 ggggcttgcc atctggggtg cggcggtcga cggccgggcg cccgcgggct tcgcggggct
     2941 cgccatcggc ctcgtggtcg ccgccgacat ctgggtcgga ggcccgctca ccggtgcgtc
     3001 gatgaacccg gcgcgttcct tcggcccgtt cctcggcgac acgttgttcg gcggttccgc
     3061 cgccgattgg gccaagttcc cgatctacct catcgggccg atcgtcggtc tggccctggc
     3121 agccttcgtg tacgactaca tctcagacct gaagaagaag ggcgcggccg agtaagcggc
     3181 agtccggccg ccaggccggg aggcccgaac gcccgggtcg tgtgcggatg aggcgggggt
     3241 gcctgccgga ggcgccggca cccccgccct catcttccct gcggcggcat cccttgagcg
     3301 agaagtgcgg ggtacgtatg aacgacaagg gcatcacgct tctcggcatc tcgggaagct
     3361 ctcgcctcaa ggccacccac ttcgccgtga acgaggcgct ccggtacgcc cacgagcggt
     3421 acggcgtgac cgtcgactcc ttcagcgccc atcgcaagac catcgggttc tgcgtccact
     3481 gcgacgcctg cgtgcgcaag aagcaggggt gcgcgttcaa ggacgacatg gaggacctgt
     3541 atcccaggct cgtgcgcgcg gacgcgtgga tcctcggcac ccccgtgtat caggggcagg
     3601 tcagcgcgca gctcaaggcg atgatggacc gtacgcgcgc ggtcgtcgcc ggcgatcccc
     3661 aggtcttccg cggcaaggta ggagctgcga tcgccgtcgg cggcgaccgc agcggcgggc
     3721 aggagccgac gctcctggcg atcgccgggt tctatctcat caacgagatg atccccgtgg
     3781 gcggcggcgc gttcggcgcc aacctcggcg cggccgtctg gtcccgcgac aagggcgccg
     3841 atggcgcccg cgacgatgaa gagggcatgc gcgcggtacg ccacgtggtg gacaggctga
     3901 tcgacgtcac gcgcacgatg cgcgcgggcg aggacgcgag gaggtcacga ggatgacgct
     3961 gaaagtcgtg tggggcatca ccggctcggg cgacaagatg cccgagaccg ttgcggccat
     4021 ggaggacctt cgagggcggc tcgacgtgga gatcacggcg gccgtctcgg tggccggcga
     4081 ggtggtgctg cgctggtaca agctccgcga gacggtcgac gagctcgcga agtcggtgaa
     4141 gatcgagaag cacgccaact cgccgttcat caccggccct ctccagatcg gccactacga
     4201 ctgcctgctg gtggcgcccg ccacggcgaa ctcggtggcc aagatcgtcc acggcatcga
     4261 ggacacgatg ctgacgaacg ccgtgggcca gacggcgaag tcgacgacgc ccatctacat
     4321 catgcccgtc gaccttcacc ccgggaccac ggtgacgatg cgtccgggcg gggagcgcct
     4381 ggagctcaag atccgcgaga tcgacgtggc gaactcgaac cggctgcgca ccatggaagg
     4441 catcaccgtg ttcgaatcgc cgtccgagat cgaagggatc ctgcgcgcct gcgcagagcg
     4501 gcgggacgcc gccccatgag ggtctcggtc gagaccggct gtcgcctgca cctcgggttc
     4561 accaatctga gcgaggacat cggccgctgc ttcggcagca tcggagtcgc gatcgaccgg
     4621 cccggcacat ccatcgtgtt cgatgagcac gatgagctgg cgatcgccgg cgaggacagg
     4681 gagcccgtgc gcgccgcggt gcgcaggttc tgcgacacct acggcgtcgc gccgcgggtg
     4741 tcgatcacgg tcaaggagca catcccggag cacgtgggac tcggctccgg cacgcagctc
     4801 gcgctggccg tcggtgtcgg cctcgccgcc gtctgcggca tcgacgccga cgtgcgcgag
     4861 atcgcctcgg ccctgcagag ggggcggcgg tccggtatcg ggatcgccgc cttccgtgcg
     4921 ggaggggtcg tcatcgacgc cggcagcagg aggcagggcg ccgatcgcgc cggcgtgccc
     4981 accgtcgtgt ggcgccacga cttccccgcg gactggtgct tcgtgatcgc cgtcccagcc
     5041 gggcgggagg ggctgagcag ccacggcgaa gagggtgtgt tcgcgtcgct gacgccgtcg
     5101 gtgcacatct cggaggaggt gtgccgcatc acccagctgc tgctgatgcc ggcgctggtc
     5161 gaacgcgaca tcgaggcgtt cgggcgcgcc ctgaccgcga tcgaccgcaa gacgggcgcg
     5221 tatttctcgg atgtccaggg tgggctgtac tcccacgagg ccaccaacga gaccgtcgcc
     5281 gcgatgctga aggaaggggc gttcggggcc ggccagagct cctgggggcc ggccgtctac
     5341 gggctcgtgc gcgcggagca agccgcgcgt ctcgagtcgc gcatgcgcga gcacctgcgg
     5401 cagagcgggc tcggagatct cgtgaccatc gcccacgggc gcaacacccc ggccaaggtc
     5461 gaggtccgca gtgacgccct atgactcctg cgccagcccg tcccgcagcc tggccagcgt
     5521 cgtcgcgaag cgcgagccgg gagcgacctt cgccgcgacg cgggcgttct cggtgaccag
     5581 cgcgagcgtg ctctcccgcg agcgcaggcc ggcccgcgtg gccgcgacga ggctctcgac
     5641 cagcagccct tcggcgcggt tgatgaggcg caccccgccg cgtgtcgtca cgtggcgcac
     5701 ggcgccggtg acgagcgtcc tgccgtcgtg cggcgtcgcc tccgcgaccg cgagctcgac
     5761 ggtcgcggtc gcgtcggtca ggcagggcgc ggagaccacc gacgcgcgcc cgaagctgag
     5821 cgcggacgga tcgtacagcg ccaggaagaa cgcccccgcg tctgccggca ggtccacgca
     5881 gaactcgccg gccgcgagca gggccggcag cgagcgcgac gtgtcgtaca gctccatgtt
     5941 cagagagcgc agatcggtgg tccacacacc cgccggggat gcgtgaggtg agccctcgag
     6001 gtctgtgcat acgacggcct cgtagatcca cccgggcgtc agggcgagct ctcggagcgg
     6061 actcatgcga caaggcctcc ggcgggcgta acgcgttgac gaggtcgaag aacgccggac
     6121 gagtatacct tccgtccttg cgccggccct cgagcgacga ctgaacggaa tcaggaggga
     6181 gaggcccatg aagaagatcc tcatccagtt cgacagcgac cgcgtcgccg ccgtgttcga
     6241 cgcggtgacc gcctacgacg ccggcatcga cgcgcttctg cagtacggcg ggatcgaacc
     6301 cggcgaggtg cgcgacctcg tctacggcgc catgttcacg cgcggccccg ccgatctgaa
     6361 gaacaccgcc gtgttcatcg gcggcatcga cgtcgccaag ggcgaagcga tcctgcgtga
     6421 ggcggtcggc tcgttcttcg accccctgcg ggtctcggtg atgctcgact ccaacggctg
     6481 caacacgacc gcggcggccg ccgtcgtgaa ggtcatctcc acggtgcccg tcgacgggca
     6541 gaaggtggtc gtgctggcgg ggaccggccc cgtgggccag cgagccgcgg ggctcctggc
     6601 cgccgagggg gcacaggtga ccctgacctc gcgcaccctc gagcgcgccc agaccgcctg
     6661 cgccgccgt
//
LOCUS       DASUMX010000300         3584 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_404638, whole genome shotgun sequence.
ACCESSION   DASUMX010000300 DASUMX010000000
VERSION     DASUMX010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3584
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404638"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1239)
                     /locus_tag="VFZ86_14145"
     CDS             complement(<1..1239)
                     /locus_tag="VFZ86_14145"
                     /inference="COORDINATES: protein motif:HMM:NF024839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HEX5643459.1"
                     /translation="MRGPVLLLAAALLTFGLLAPPASADTYTPEECLACHTSFQPGLV
                     KQYHQGDMSGAGVTCVSCHGADHAAIAAAQGSVPASTCAGCHPDQYAEFAYKDAAGDY
                     VNKHAIGWTRMTAAARYKVMPEEQRYEMCERCHNVGYVSPDGSVGKCDSCHTRHTFSA
                     EEAMEPPACGTCHMGPDHEQIDMWEKSKHGVVYGTEKERAGGDPSRAPTCVTCHMPEL
                     EKGKGQSLTHNVSTNLTIGTVAQGARLPGVALPVPMRTISNWDFDVRRNKMVAVCDDC
                     HAPSFVRRNLEGADEIKADVDTLLWDPVMRIRGLWWDGLLDPMPANRAPNPNFGQALV
                     LGGQQLYGGTSAIEQLFFYTYKYDHVNTFKGAYHINPDYSHWFGWSEVNQDLDLIKGE
                     ESKMRRLAAQYDPGFVVTTEK"
     gene            complement(1362..>3584)
                     /gene="rlmKL"
                     /locus_tag="VFZ86_14150"
     CDS             complement(1362..>3584)
                     /gene="rlmKL"
                     /locus_tag="VFZ86_14150"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: protein motif:HMM:NF008748.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HEX5643460.1"
                     /translation="LPVASGTAADGDDLYKTAQSVDWSRHLAVDDTLAVDFTGVNDRI
                     RDTRFGAVRIKDAIVDQFRASSGGLRPSVNARAPDVRVNAHLASTRVAISIDLSGQSL
                     HRRGYRADKVQVEAPLKENLAAGLLLYAAWPSEAAAGASLLDPLCGSGTLPIEAALMA
                     ADVAPGLLRAEGETKPGAAPAFGFLRWRGHDAVLWDALIAEARERRGAGMKRLAGARP
                     GLVIRGSDRDQRAVKVARDFVKRAGLHGLVTIEHAQLETVAAPTERGLLATNAPYGER
                     LDVADTEVVYQQLGERLREAFPGWSAVVLAGDRRQLKAVGLAPKRETDLRNGPLECVL
                     AYYEAGAAPTGAAAPRTARAEARSSRKAAAPDATGAPAAAAERPAVSAPAPETEAPSG
                     PRRALGGGAEYLANRLRKNQRRLARRLQREGITCYRLYDADLPEYNLVVDVYGEWVHV
                     QEYAAPPEIDPGKAKRRLAEAVEVIGAVLETPPQRVVLKERRRQRGAAQYERRGDAGK
                     HLEVSEDELTYLVDLNTYLDTGFFIDQRLTRRLVRRLAGGRRFLNLFSYTGTMTVNAL
                     AGGSPASTTVDLSATYLTWAERNLAANGFATATEVWPAPSGKPDESSVTSAGAAAAGS
                     GPHTLVQADCLRWVAEAGGAYDLIWLDPPTFSNSKKMGRATFDVQRDHADLIRMTARR
                     LLAPGGILLFATNLRNFRLDHAELRGLGVKDLSRATLAFDCERGANRHHVFQIEPGAR
                     "
BASE COUNT          446 a         1223 c         1353 g          562 t
ORIGIN      
        1 cttctccgtg gtcacgacga agccagggtc gtactgggcg gccagccggc gcatcttgga
       61 ctcctcgccc ttgattaggt cgaggtcctg gttgacctcg gaccagccga accagtggct
      121 gtagtcggga ttgatgtggt aggcgccctt gaacgtgttc acgtggtcgt acttgtaggt
      181 gtagaagaag agctgctcga tcgccgacgt gccgccgtag agctgctgtc cgcccagcac
      241 cagcgcctgc ccgaagttgg ggttcggggc gcggttcgcg ggcatcgggt cgagcaggcc
      301 gtcccaccac aggccgcgga tgcgcatcac ggggtcccag agcagggtgt ccacgtcggc
      361 cttgatctcg tcggctccct cgaggttccg gcggacgaag gagggcgcgt ggcagtcgtc
      421 gcagacggcg accatcttgt tgcgcctcac gtcgaagtcc cagttggaga tcgtgcgcat
      481 cggcaccggg agcgccacgc ccggcaggcg ggcgccctgc gcgacggtgc cgatcgtgag
      541 gttggtcgag acgttgtggg tcagggactg gcccttaccc ttctccagct ccggcatatg
      601 gcaggtgacg cacgtcggcg cgcggctcgg gtcgccgccc gcgcgctcct tctcggtccc
      661 gtagacgacg ccgtgcttgc tcttctccca catgtcgatc tgctcgtggt ccgggcccat
      721 gtggcaggtg ccgcatgcag gcggctccat ggcctcctcg gccgagaagg tgtggcgcgt
      781 gtggcagctg tcgcacttgc ccaccgagcc gtccgggctg acgtagccga cgttgtggca
      841 gcgctcgcac atctcgtagc gctgctcctc gggcatgacc ttgtagcgcg cggccgcggt
      901 catgcgcgtc catccgatgg cgtgcttgtt gacgtagtcg ccggcggcgt ccttgtacgc
      961 gaactcggcg tactggtcgg ggtggcagcc ggcgcaggtg ctcgccggca cgctgccttg
     1021 ggcggcggcg atggcggcgt ggtcggcgcc gtggcacgag acgcaggtga cgccggctcc
     1081 cgacatgtct ccctggtggt actgcttcac caagcccggc tggaacgagg tgtggcaggc
     1141 gaggcactcc tccggcgtgt aggtgtcggc agaggcaggc ggcgccagca agccgaacgt
     1201 gagcagcgct gccgccagca gcaggaccgg tcccctcatg gtgcaccccc accgtacgta
     1261 gttggtcacc cccaagtatg gacccgtgtc aacggccgcc gaattgacct tcgtcggatg
     1321 ctgagggtgg cggcggccgg cgcgcggcgc gcgcgaggag gtcagcgggc gccgggttcg
     1381 atctggaaca cgtggtgccg gttggctccg cgttcgcagt cgaaagcgag ggtcgcgcgc
     1441 gagaggtcct tgacgccgag gccacggagt tcggcgtggt cgaggcggaa gttccgcagg
     1501 ttggtggcga agagcaggat gccgccgggg gcgagcaggc gccgggcggt catccggatg
     1561 aggtcggcgt ggtcgcgctg gacgtcgaag gtggcgcgac ccatcttctt ggagttgctg
     1621 aacgtcggcg ggtccagcca gatgaggtcg taggcgccgc ctgcctcggc cacccagcgc
     1681 aggcagtcgg cctggacgag ggtgtgcggg ccggatcccg ccgcggccgc tccggccgac
     1741 gtcacggagg actcgtccgg cttgccgctc ggcgccggcc agacctcggt cgcggtcgcg
     1801 aagccattgg cggcgaggtt tctctccgcc caggtcagat aggtcgcgga gaggtcgacg
     1861 gtggtggagg ccggtgatcc gccggccagg gcgttcaccg tcatggtgcc ggtgtacgag
     1921 aagaggttga ggaaccggcg gccgccggcc agccgccgga ccaggcggcg cgtgagccgc
     1981 tggtcgatga agaagccggt gtccaggtac gtgttcaggt cgacgaggta cgtgagctcg
     2041 tcctcgctca cctcgaggtg cttgccggcg tcgccgcgcc gctcgtactg cgccgcaccg
     2101 cgctgccggc ggcgctcctt gagcaccaca cgctgcggcg gcgtctcgag cacggcgccg
     2161 atgacctcga ccgcctcggc aaggcgccgc ttggccttgc cggggtcgat ctcgggcggc
     2221 gccgcgtact cctggacgtg cacccactcg ccgtacacat ccacgacgag gttgtactcg
     2281 ggcaggtcgg cgtcgtagag ccggtagcag gtgatgccct cgcgttgcag gcgcctggcc
     2341 agccggcgct ggttcttgcg caaccggttg gccaggtact cggcgccgcc gccgagcgct
     2401 ctgcgcggac cggagggagc ctccgtctcg ggcgccggtg cgctcactgc cggcctctcg
     2461 gcggccgcgg ccggcgcgcc cgttgcgtcc ggcgccgccg ccttgcgtga ggaccgggcc
     2521 tcggctcgcg cggtgcgcgg cgcggccgca ccggtgggcg ccgcgccggc ctcgtagtag
     2581 gcgagcacgc actcgagcgg gccgttgcga aggtccgtct cccgcttggg cgcgaggccc
     2641 acggccttga gttgccggcg gtcgccggcg aggaccacgg cgctccaccc cgggaaggcc
     2701 tcccggaggc gctcgccgag ctgctggtag acgacctcgg tgtcggcgac gtcgagccgc
     2761 tcgccgtagg gggcgttggt ggcgaggagg ccgcgctcgg tgggcgcggc gacggtctcc
     2821 agctgggcgt gctcgatcgt caccaggccg tgcagcccgg ctcgcttgac gaagtcgcga
     2881 gccaccttca cggcgcgctg gtctctgtcc gacccgcgga tgacgaggcc cggccgcgct
     2941 cccgcgagcc gcttcatacc ggcgccccgg cgctcgcgcg cctcggcgat gagcgcgtcc
     3001 cagagcacgg cgtcgtggcc gcgccagcgc aggaagccga aggccggcgc ggcgcccggc
     3061 ttggtctcac cctccgcgcg cagcaggccg ggcgcgacgt cggcggccat cagcgccgcc
     3121 tcgatcggca gcgtccccga cccgcacagc gggtcgagca ggctggcgcc ggcggcggcc
     3181 tcgctcggcc aggccgcgta gagcagcagg ccggcggcga ggttctcctt gagcggcgcc
     3241 tccacctgga ccttgtccgc acggtagccg cgccggtgca ggctctgacc gctcaggtcg
     3301 atcgagatgg ccacgcgcgt gcttgcgagg tgcgcgttga cgcgcacgtc cggcgcgcgc
     3361 gcgttcaccg acggccgcag gccgccggag gaggcgcgga actggtcgac gatcgcgtcc
     3421 ttgatgcgca ccgcgccgaa gcgcgtgtcc cggatcctgt cgttgacgcc ggtgaagtcg
     3481 accgccagcg tgtcgtccac cgcgaggtgg cgcgaccagt cgacggactg cgcggtcttg
     3541 tagaggtcgt cgccgtcggc ggccgtcccg ctcgcgaccg gcag
//
LOCUS       DASUMX010000301         6558 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1986355, whole genome shotgun sequence.
ACCESSION   DASUMX010000301 DASUMX010000000
VERSION     DASUMX010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 6558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6558
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1986355"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(266..889)
                     /locus_tag="VFZ86_14155"
     CDS             complement(266..889)
                     /locus_tag="VFZ86_14155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011813878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5643461.1"
                     /translation="MDARFSTPPGGHAVVYDALMWPLERTALGAWRRRLAAGARGRVL
                     EIGTGTGAQLAWYAPGTRVTALEPDAGMLARARRRATRAAASVTVVEGRAEELPFADA
                     GFDAAVSAFALCTVADPAAALAEVRRVLVPGGTLQLLEHVHLPWEPGRALQSRAAPAW
                     ASVAGGCRLDRDTLRSAREAGFVVVSARTHVAGWVVQASLRAPSTGA"
     gene            973..1398
                     /locus_tag="VFZ86_14160"
     CDS             973..1398
                     /locus_tag="VFZ86_14160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643462.1"
                     /translation="MSGLFHFMQYDKHAPGAAIDDETRQELGRVANLFLVSAITMIAL
                     FAELFAQALEAAFIPQAVHTVLGLVFLVGWAATVVYGVYVTFTARRWLWLALCVIPPT
                     SPPAALVYAWIRRREIEREVLGDEPSPPARRSRGGSTKR"
     gene            1547..2575
                     /locus_tag="VFZ86_14165"
     CDS             1547..2575
                     /locus_tag="VFZ86_14165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HEX5643463.1"
                     /translation="MVLALYLLLPRLVDTQQTLDYISNASYVLLGLAVALEIAALGGY
                     ANLFRYILRVLDIRMRLREVWAITLSGLAVSHILSAGGVGGWVVTYNALMKHKVPHGI
                     IFVAIAAQQFFNYVVLWFFFALAIVYLILVRGSDSIFGYLVGIALIALILWLTLYGVY
                     LYNRPTRLRVRATQIAHLVNRVWRREVVKERHIDGWIDNLLIGMRRMVRYRGSFRTTA
                     VLACTFWLFDMLCLWCTFQAFGYTIGLGHLLVAYVVAYSIGTLAPTPGGLGAVEGLLI
                     ALFVSFGVPSAVAVAVVLVYRIINFWLPIPPGFIAYAVVRPGRTPVMEGEVEAAAGEA
                     PRELDARR"
     gene            complement(2600..4234)
                     /locus_tag="VFZ86_14170"
     CDS             complement(2600..4234)
                     /locus_tag="VFZ86_14170"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX5643464.1"
                     /translation="MSAGPAGRRRSVSLAGVLLVAILVLALALRLKGITWGLPYSFVN
                     ADESTVVPKAAAAARGHLNPQFFFYPSFYFYLAGAVYVLAAPVWWLLGNGNLLSLTSL
                     VVDPGPYFLLARLLSVAMGTASVYLVYRLGRVAFGRPVGLLAALFLAVAPLHVAYSHM
                     AVTDVTAVALALLALVLLQRAAERSAARWLVLGAVAAGLATSTKYNLGMLVLPATVAA
                     VLTCRAEVARRAAAGGRAALVWLRMLALRVYVPMLLAFVVASPFVVLDAPHFVRDFRR
                     QTQIMDRGWLGFEHVGNGFWYNVTPNLTGAIGVVLVVLGAAGLGWALWRRTRLDLMIA
                     PYVIVYFAYIGTWKELADRYLLVIAPLLILLGVRLVVDLALLRPRLRWIAVPAAAVVL
                     AFGFVPPLLDSIAYDRTLSGTDTREVAGRWIESGLPAGALIAVENYGPPLVREGQLHH
                     YRAAGLDPVAFRLVRLKLPLPGMPERTRDLDRLREQGVEYVVTSSRVRDRVLAAAADY
                     PAPAEFYRRLDAEAELVREFRPGPDERGPVLKLYRL"
     gene            complement(4285..4596)
                     /gene="hisI"
                     /locus_tag="VFZ86_14175"
                     /pseudo
     CDS             complement(4285..4596)
                     /gene="hisI"
                     /locus_tag="VFZ86_14175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013146378.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
     gene            complement(4596..5297)
                     /gene="deoC"
                     /locus_tag="VFZ86_14180"
     CDS             complement(4596..5297)
                     /gene="deoC"
                     /locus_tag="VFZ86_14180"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEX5643465.1"
                     /translation="MQAEEVAKTIDHTLLRADGTSADVDALCREAATWHFAAVCLFPH
                     FVPLASGLLKGTDVKTCTVISFPFGADTPRVKILAADEAVRRGADEVDVVINVPGLLS
                     GEFGLVRDELSGLVDAVRVRGDNLGRTVIVKAIIETCYLDDKLKRLACKICEQAGVDF
                     VKTSTGLGPHGATVADVELLRDCLDAHVGVKASGGIRTAADLERMVSAGAVRVGTSAG
                     VAIMRESLAAGRAEA"
     gene            complement(5377..6261)
                     /gene="era"
                     /locus_tag="VFZ86_14185"
     CDS             complement(5377..6261)
                     /gene="era"
                     /locus_tag="VFZ86_14185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEX5643466.1"
                     /translation="MTEFRSGFVAVFGRPNVGKSTLVNALVGRKVSIVSDHPQTTRRR
                     ITGVVTLDDCQLVLLDLPGFQKPFDSLTRRMQTTVDDALEEVDAALVLLNATETFGPG
                     DRYIAQAVAAAGVPAVVVLNKIDLVDGPRLGVRLEEARSLSDAREVLPVSALRGDGLA
                     DVLARLAAAMPPGPVYFPEGQQTDQPLELLVAELVREQALRLTRDEVPHAIAAEVTSI
                     TERSDRPLVEIEASVIVETESQKAIVVGKGGRVVKAIGSGARHEIEAILGVHVFLDLR
                     VKVRKRWRRDDRYVDHLL"
     gene            complement(6254..>6558)
                     /locus_tag="VFZ86_14190"
     CDS             complement(6254..>6558)
                     /locus_tag="VFZ86_14190"
                     /inference="COORDINATES: protein motif:HMM:NF012601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEX5643467.1"
                     /translation="SHHGANVESAASPAGLCAERAALAALVVHGERELRYVAVAALDG
                     SDCLPCGLCLQTLAEFGDPEIVARTGGELRVVRLRELLAAPFALPEASAPQGGGDD"
BASE COUNT          938 a         2405 c         2341 g          874 t
ORIGIN      
        1 gcggcgcgtc ggacgcctcg ttgttcgttc gggaccgaac tcaaccaaat gcaacggcgt
       61 tctgcaaccc aaagcgcctg ccaaagaccg cgccgagaag gcgcgccggc gctcccggcg
      121 agcctcctca tgcgctccac gcgcgaccct gcaggctcgg ggatcgggcg acactcatgt
      181 tctggtcgaa ggtctgctca ggcagtgcac gaggcgcgtc cacccagtgc atgagacgac
      241 gctcagcgac tgcttgaggg tcgtctcaag cgccggtcga gggagcgcgg agactggcct
      301 ggaccaccca gccggcgacg tgcgtacgcg cgctcacgac gacgaacccc gcctcgcgtg
      361 cggagcgcag cgtgtcccgg tcgagacggc agccgccggc gaccgacgcc caggccgggg
      421 ccgccctgct ctggagggcc cgccccggct cccacggcag gtggacgtgc tcgagcagct
      481 ggagcgtccc acccgggacg aggacccggc ggacctcggc gagcgccgcc gccgggtccg
      541 ccaccgtgca cagcgcgaac gcgctcacgg cggcgtcgaa ccccgcatcg gcgaacggca
      601 gctcctcggc gcggccctcc acgacggtga cgctcgcggc ggcccgcgtc gcccgccggc
      661 gcgcccgcgc gagcatgccg gcgtccggct cgagtgcggt gaccctcgtg cccggcgcat
      721 accaggcgag ctgcgcaccg gtgccggtgc cgatctcgag cacgcgcccg cgcgcgcccg
      781 cagcgaggcg tcgccgccag gcgccgagcg ccgtgcgctc gagcggccac atgagggcgt
      841 cgtagacgac ggcgtgtccg ccgggagggg ttgagaagcg tgcgtccatc ctgaagactg
      901 acctggtcct ttctgcgtcc gtccggtact tgccccgccg gccggcgcca cacgcatcgg
      961 ggcggcggcg ggatgtccgg cctcttccac ttcatgcagt acgacaagca cgcgcccggc
     1021 gcggccatcg atgacgagac gcgacaggag ctcggccgcg tcgccaacct cttcctcgtc
     1081 tcggcgatca cgatgatcgc cctcttcgcc gagctcttcg cgcaggcgct cgaggcagcc
     1141 ttcatcccgc aggccgtcca caccgtcctc gggctcgtct tcctggtcgg ctgggcggcg
     1201 accgtggtgt acggcgtcta cgtgaccttc acggcgcggc gctggctctg gctcgcgctc
     1261 tgcgtcatcc cgcccaccag cccgcccgcg gcgctcgtct acgcctggat ccgccgccgg
     1321 gagatcgagc gcgaggtcct cggcgacgag ccctcgccgc ccgcgcgccg gagccgcggc
     1381 ggcagcacga agcgctgagc ggcccacagg cgtgctcccc ggccgcccga gcgtgcctgc
     1441 ggcccctctg tggtaacgtc ttggacttgg cgggagggcc cgccgcgtcc gtccttgggg
     1501 agacgccatc aagaagacgc tccgccacat catcgtcttc gccgtcatcg tgctggcgct
     1561 gtacctgctg ctgccgaggc tggtcgacac gcagcagacg ctcgactaca tctcgaacgc
     1621 gagctacgtg ctgctcgggc tcgcggtcgc gctggagatc gcggcgctgg gcggctacgc
     1681 caacctgttc cgctacatcc tgcgcgtgct cgacatccgc atgcgcctgc gcgaggtgtg
     1741 ggcgatcacc ctgagcggcc tcgccgtcag ccacatcctc tcagcgggcg gcgtgggcgg
     1801 ctgggtggtc acctacaacg cgctgatgaa gcacaaggtg ccgcacggca tcatcttcgt
     1861 cgccatcgcc gcgcagcagt tcttcaacta cgtggtcctc tggttcttct tcgccctggc
     1921 catcgtgtac ctgatcctgg tgcgcggcag cgactccatc ttcggctacc tggtcgggat
     1981 cgccctgatc gcgctcatcc tctggctgac gctgtacggc gtgtacttgt acaaccggcc
     2041 tacgcgcctg cgagttcgcg ccacgcagat cgcccatctg gtgaaccggg tctggcgccg
     2101 cgaggtggtc aaggagcggc acatcgacgg ctggatcgac aacctgctga tcggcatgcg
     2161 ccgcatggtc cggtaccgcg ggtcgttccg caccacggcg gtgctcgcct gcacgttctg
     2221 gctcttcgac atgctctgcc tctggtgcac cttccaggcc ttcggctaca ccatcggcct
     2281 gggtcacctg ctcgtcgcct acgtcgtcgc ctactccatc gggacgctgg cgcccacgcc
     2341 gggtgggctg ggcgccgtcg aaggcctcct gatcgcgctg ttcgtgagct tcggcgtgcc
     2401 ctcggccgtg gccgtcgccg tcgtgctcgt ctatcgcatc atcaacttct ggctgccgat
     2461 cccgccgggg ttcatcgcct acgcagtcgt ccgtcccggg cgcacgcccg tcatggaggg
     2521 cgaggtggag gcggcggccg gcgaggcgcc gcgcgagctc gacgcccggc gctgacccgg
     2581 ccggcgggcg cgcgggacgc tagaggcggt agagcttgag gaccggcccg cgctcgtcgg
     2641 gtccgggccg gaactccctg acgagctccg cctcggcgtc caggcgccgg tagaactccg
     2701 ccggcgccgg gtagtccgcg gcggccgcca gcaccctgtc gcgaacgcgc gagctcgtca
     2761 ccacgtactc cacgccctgc tcccgcaggc ggtccaggtc gcgcgtgcgc tcgggcatcc
     2821 cgggcagggg cagcttgagc cgcacgaggc gaaaggcgac cgggtccagg cccgccgcgc
     2881 ggtagtggtg caactggccc tcgcggacca gcggcgggcc gtagttctcg acggcgatga
     2941 gcgcgccggc cggcaggccc gactcgatcc agcggcccgc gacctcgcgc gtgtccgtgc
     3001 cgctcagggt gcggtcgtag gcgatcgaat ccagcagcgg cggcacgaac ccgaacgcca
     3061 gcaccaccgc ggccgccggg acggcgatcc agcgcagtcg tggtcgcagc agggcgagat
     3121 cgacgaccag gcgcaccccg agcaggatga gcagcggcgc gatcacgagg aggtagcggt
     3181 cggccagctc cttccaggtc ccgatgtacg cgaagtacac gatgacgtac ggcgcgatca
     3241 tgaggtcgag gcgggtgcgc cgccacaggg cccagcccag gccggcggcg ccgagcacga
     3301 cgagcacgac gccgatcgcg cccgtcaggt tgggcgtcac gttgtaccag aacccgttgc
     3361 cgacgtgctc gaacccaagc cagccgcggt ccatgatctg cgtctggcgc cggaagtcgc
     3421 gcacgaagtg cggcgcgtcg aggacgacga acggcgacgc cacgacgaac gccaggagca
     3481 tcggcacgta gacgcgcagc gcgagcatcc gcagccagac gagcgcggcg cggccacccg
     3541 cggccgcgcg ccgcgccacc tccgcacggc aggtgaggac ggcggccacg gtcgcgggca
     3601 gcacgagcat cccgaggttg tacttggtgg acgtcgccag gccggcggcg accgcaccga
     3661 gcacgagcca gcgggcggca ctgcgctcgg cggcccgctg cagcagcacc agggcgagca
     3721 gcgccagcgc gaccgcggtc acgtcggtca ccgccatgtg cgagtaggcc acgtggagcg
     3781 gcgccaccgc gaggaacagc gccgccagga ggcccacggg gcggccgaag gcgacccggc
     3841 cgagccggta cacgaggtac acggacgccg tgcccatcgc cacggagagc aggcgggcca
     3901 ggaggaagta cggtcccggg tcgacgacca gggaggtcag cgacagcaga ttcccgttgc
     3961 cgagcagcca ccacaccggc gccgccagca cgtacaccgc tcccgccagg tagaagtaga
     4021 acgacggata gaagaagaac tgcgggttca ggtgcccgcg ggcggcggcg gcggccttgg
     4081 gcacgaccgt gctctcgtcc gcgttgacga agctgtaggg caggccccag gtgatgccct
     4141 tgagccgcag cgccagggcg aggacgagga tggcgacgag gaggacgccg gcgaggctga
     4201 cgctccggcg ccggcccgca gggccggcgc tcacccgccg ccggcggcgg gccccgtcgc
     4261 cgcgccgccg ctggcctcgt cgcggaagca gctcaccgcg ccggtgtggc aggccgggcc
     4321 ggcgggctcg acggacagga ggacggtgtc gtggtcgcag tccagccgcg cggacaccac
     4381 gcgctgcacg ttgccgctgg tgccgccctt gtgccagagc tcccgccggc tgcggctcca
     4441 gaagtgcgcc tcgccggagc tcagcgtggc gcgcagcgcg gcttcgttcg cgtacgcgac
     4501 catgagcacc tcgaggctgg ccgcggcctg gaccacgcag ggcaccaggc cgtcggcatt
     4561 gtagcgcacg agcccgagga acgtggcgtg gtccatcacg cctcagccct ccccgccgcc
     4621 aggctctccc gcatgatcgc gacgccggcg ctggtgccca ccctgacggc gccggcgctc
     4681 accatgcgct ccaggtccgc cgccgtgcgg atgccgcccg aagccttgac gccgacgtgc
     4741 gcgtccaggc agtcacgcag cagttccacg tccgcgacgg tcgcgccgtg cggcccgagc
     4801 ccggtggagg tcttcacgaa gtccacgccc gcctgctcgc agatcttgca ggccaggcgc
     4861 ttcagcttgt cgtccaggta gcaggtctcg atgatcgcct tgacgatcac ggtgcggccc
     4921 aggttgtcgc cgcgcacgcg caccgcgtcc accaggccgg agagctcgtc gcgtacgagc
     4981 ccgaactcgc cggagagaag acccggcacg ttgatgacca cgtccacctc gtccgcgccg
     5041 cgccgcaccg cctcgtcggc ggcgaggatc ttgacgcgcg gcgtgtccgc gccgaacggg
     5101 aacgagatga cggtgcaggt cttgacgtcc gtgcccttga gcagccccga cgcgagcggc
     5161 acgaagtgcg ggaacaggca gacggcggcg aagtgccagg tagccgcctc ccggcacagg
     5221 gcgtccacgt ccgcgctcgt gccgtcggcg cgcagcagcg tgtgatcgat ggtcttggcg
     5281 acttcctcgg cctgcacgcc tcgacctccc atggccggca tcggacgggc tggaacgcgc
     5341 agagtatagc aacaggccgg gcggggccgc gaagcgctac agcaggtggt cgacgtagcg
     5401 gtcgtcgcgg cgccagcgct tgcgcacctt gaccctcagg tcgaggaaca cgtgcacgcc
     5461 gaggatcgcc tcgatctcgt gacgcgcacc gctgccgatg gctttgacca cacggccgcc
     5521 cttgccgacg acgatcgcct tctgcgactc cgtctcgacg atgacgctgg cctcgatctc
     5581 gaccagcggg cggtcgctgc gctcggtgat cgaggtgacc tccgcggcga tggcgtgggg
     5641 cacctcgtcg cgggtcagcc gcagggcctg ctcgcgcacg agctccgcca cgagcagctc
     5701 cagcggctgg tcggtctgct gcccctcggg gaagtacacg gggcccgggg gcatggccgc
     5761 ggccagccgg gcgagcacgt cggcgaggcc gtcgccgcgc agcgccgaga cgggcaggac
     5821 ctcgcgggcg tcggagagcg agcgcgcctc ctcgaggcgg acgccgagcc gcggaccgtc
     5881 cacgaggtcg atcttgttga gcacgaccac ggccgggacg ccggcggcgg cgaccgcctg
     5941 cgcgatgtag cggtcgccgg ggccgaacgt ctcggtcgcg ttcagcagca ccagcgccgc
     6001 atccacctcc tccagcgcgt cgtccacggt ggtctgcatg cgccgggtga gcgagtcgaa
     6061 cggcttctgg aacccgggaa ggtccaggag caccagctgg cagtcgtcca gcgtgacgac
     6121 cccggtgatg cgccgccgcg tggtctgggg atggtcgctg acgatgctga ccttgcgccc
     6181 cacgagcgcg ttgaccagcg tcgacttgcc gacgttgggg cgcccgaaga cggcgacgaa
     6241 gccgctgcgg aactcagtca tcgccgcctc cctgcggggc gctcgcctcg ggcagcgcga
     6301 acggcgccgc gagcagttcg cgcaggcgca cgacgcgcag ctcgccgccg gtgcgggcga
     6361 cgatctccgg gtcgccgaac tccgccagcg tctgcaggca gaggccgcag ggaaggcagt
     6421 cggacccgtc cagcgcggcg acggcgacgt agcgcagctc gcgctcgccg tggacgacga
     6481 gcgcggcgag cgcggcgcgc tcggcgcaca ggccggcagg ggaggcggcg ctctcgacgt
     6541 tggcgccgtg atggctgc
//
LOCUS       DASUMX010000302        13196 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1410247, whole genome shotgun sequence.
ACCESSION   DASUMX010000302 DASUMX010000000
VERSION     DASUMX010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 13196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13196
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1410247"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..923)
                     /locus_tag="VFZ86_14195"
     CDS             complement(<1..923)
                     /locus_tag="VFZ86_14195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015154950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX5643468.1"
                     /translation="MAPASAHRTRDLGIIALLVLGPIWGYGWVATKIALEYSDALTFA
                     ALRVTLSAACLFLVMLVLRKPLRPPPLGWTMLIGLLQTTLFVGFVITALHDTGAGRVS
                     VITYTMPFWLLLLAWVFLGERLRGVQWLAVALAFAGLVLVVRPWELAGAASGILTLLG
                     GLSWAASALAVKLMQRRHRVDVLSLTTWQMAFGSVPLIVAALLTYSGGPEWTLSFWWG
                     LIYTVVLANAVAWFLWLYALHALPAGAAGLGTLSIPVVGVAAAWIQLGEVPTLVEGIG
                     MVLIIAALAVLAAHGLLAGRRGTAAAGEPDVR"
     gene            complement(1001..1942)
                     /locus_tag="VFZ86_14200"
     CDS             complement(1001..1942)
                     /locus_tag="VFZ86_14200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015154950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX5643469.1"
                     /translation="MPATSSKRTRDLGILALAVIAPIWGYSWVVSKVALEYSEPFTFA
                     ALISSLGALCLFVVLVVTRRPLRPPPLGWTALIGLLQTALFNGMATAALTVGGAGKVS
                     VLVYTMPFWLLTLAWLFLGERLRGLQWPAVGLAFTGLVLVVRPWDIGGALSGVLACGA
                     GLAWAGSALLVKLLQRRTRCDTLSLTAWQLALGSVPLIAVAVLVEGTAPEWTGVFVAC
                     LAYSILLANAACWALWIFALRALPAGAAGMGTLAVPVVGVVTAWLQLGEAPAVVEGVG
                     MALIIAALAVLAATGVKAGRGGGPAGGEEPALLPIID"
     gene            complement(2048..2524)
                     /locus_tag="VFZ86_14205"
     CDS             complement(2048..2524)
                     /locus_tag="VFZ86_14205"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX5643470.1"
                     /translation="MAHLTNDFHVGVPVEVFDGVVKDPRRWPTFWVGMDASPRVFGDG
                     SPGTKAEFFQHMMGVKVRMVDRTVEERHNPDGSSDWRWQIDGAMSGTISCHHEPRADG
                     TDVTTTFDYDVPRRFGGRAADRLLFEKRMRRDFEDSMENLRLLAETSDVPAAIATA"
     gene            2783..3325
                     /locus_tag="VFZ86_14210"
     CDS             2783..3325
                     /locus_tag="VFZ86_14210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014447284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HEX5643471.1"
                     /translation="MDVLSGLTLAAPAGLNAYIPLLAVALAERLGWVHLREPFDGIGS
                     WWVIGVIAVLLVVEVVADKVPAVDHVNDVIQTVVRPAAGGLLAVSASTQGTVEPLLLL
                     IAGVLIAGGVHAVKATARPAVNVTTAGVGAPVVSTGEDILAVIGTVLALLAPVLAAVL
                     IVAAVLLAVVLLRRRRQRAA"
     gene            3350..4708
                     /locus_tag="VFZ86_14215"
     CDS             3350..4708
                     /locus_tag="VFZ86_14215"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5643472.1"
                     /translation="MSSARPTRSFGWYVVGASFVVLFLNAGARLMIGVLFKPVIADFG
                     WSRSAVSAAVLVNLAVFAVAIVVAGRLFDRYGPRWVILVSSVLFSGGLMLTAVMTDLW
                     QFFLFYGVISAAGLGGTSAPIFGAIVSRWFERGRGTAVSLAMAGTSLGQFALVPLFTL
                     FLMDQGWRATVFWVGVLCLVVNVVLVFAVLSGDHGDPGKARDRHEASARAAATPAAPA
                     EAGVAVPRSLTLREAMRTASFWYFTVAMFICGAGDFLLTTHLVPMATDHGVATSTAVA
                     MLAWFGLLSLGGILLAGPASDLIGDRVPIAIAFGLRVALFLLVFRYQNAASFWALALG
                     FGFTFLVTAPLTTTLMGRLYGFAHIGLLGGFITTVHHVGGGIWAYLGGAVYDATGGYT
                     PAMAISAVASAIALVLTLLIKEVRHVAPEEPHGQDSAPAGEPRFAGAATGARVVTRTR
                     GM"
     gene            4753..5013
                     /locus_tag="VFZ86_14220"
     CDS             4753..5013
                     /locus_tag="VFZ86_14220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643473.1"
                     /translation="MSDIDDHVIPYEEAVRRRRAREAQKERSAAAGLKISDAMSHLLE
                     QAGTQDDEGHIPAHLRDIVEREFADGEPPPDRPSAGRPGGDA"
     gene            5085..5645
                     /locus_tag="VFZ86_14225"
     CDS             5085..5645
                     /locus_tag="VFZ86_14225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643474.1"
                     /translation="MRLIKVLVLVGVVLLVGSSVALAGDRLAQRTQDRDRTPVAAKTC
                     TPAPDATCAAVPARDRDRDRDGSAGEQTATGDQVRDRDRTGDCDGTGDGQQAQTRQRD
                     GQQTQTQTQTQTQSQKQTQTQTQKQTGDQARVGQPAGAGTGNGSCDGSGSGSSNGNGA
                     GDGTGPVAGSGECDGSGAGAGNGAGH"
     gene            complement(5782..8082)
                     /locus_tag="VFZ86_14230"
     CDS             complement(5782..8082)
                     /locus_tag="VFZ86_14230"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminaseTgpA domain-containing protein"
                     /protein_id="HEX5643475.1"
                     /translation="MNGRRALFYVVGFAGLAAVSAVVAGRVTDPSIAPLLLATAAVAT
                     LAGAPGLVRRRAWALALLLLPLGAYLLMRAQVPLPPQASGVPAHLAFYADQLGAGGIA
                     YAYDVFPLDVSSQAGLRLLLSLVVYVTVGVAAFLALSLRRPLPAVVMLLALAGFGFTT
                     DSSARDPWAAIAFVLFAGWMLSLSRSLGQQRRHATDALAGGVTAVLAALLALSIIGTT
                     AVEAGRPLHDWRSWDITGPRTAKFRFDRMQNYPRLLEPANDVVVMRVRSPIASYWRAS
                     VLADFTGTTWRGGAPDGRELRPQARGGDWVYEAPQIRPAPQGRLVTQRFEIEGAYTDR
                     LFVGGWAVEVRSALPLDLQMTSAAAVAVEPPRGPSLDYSVAAVVPDLEPTDLVGRGRY
                     YPGDVTRSFTALPFPAGPAAGGPSSESAWRAEAAAVPAGREWLGLYALNERVVGGETD
                     PFRVALAVEQYLRSNHDYSLRPPDAGFDSPYAEFLFATRIGYCQHFAGAMAAILRFNG
                     IPARVAVGFTAGEEERNGIWVVTRNDAHAWVEAYFPGVGWAQFDPTPGREIPSTSTAP
                     ASGPDAAAAAGLDGAGASPAPEAAAVDGRARVADPSRQVDETVVAEEPGGRAPWLVVS
                     LLAAAVAWPAGRALLRGRGLRRGSREDRLRASVALLYADLRDHGAEAKPSQTLDETAR
                     YLSDHLGVDAGDLPDRVQAVEFGSRRVSDADLKDLGALRRRVRRRLRERSGRLTALLA
                     LYGVRPRRRRGSRLPVARRARPATRS"
     gene            complement(8079..9350)
                     /locus_tag="VFZ86_14235"
     CDS             complement(8079..9350)
                     /locus_tag="VFZ86_14235"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX5643476.1"
                     /translation="MTRPTARGVALLAVAAATYVAARVVGTWELYLIALALAAMAVVA
                     WLLVHSGSRRLLVDRRVAPEGPVAGDPLKFFFHVRSGRRLTGLHVTLEGATGALGSHG
                     PVVVDEPGTHGERTVTAGPWPARRGVHRLPPFTAVVEDQLGLVRVRRPAGEPLRLTVA
                     PRLDELTSCAPCRAAGGVRNRGGRRRLPTRDAWELHGVRPHVPGEPLNRVDWKSTAKT
                     GSLMLREMEAASEDDLTVLLSGAAACDAGEAPDSAFEAAVRAAGSMAAFTLRTGHGVT
                     LLLPEHDWRPIRLTPDAASRRRMLRVLAEAEPQGPSRLGPSLQAIVGGRTQVRRGVLA
                     LVVLCLDGGLVDALRRLRTQGVPVSVVHVTGEVTGGDASDGRSAPGAATDAEMRQALV
                     AAGVRYVRLHRDDDLGSALSAGAPERLARAR"
     gene            complement(9347..10345)
                     /locus_tag="VFZ86_14240"
     CDS             complement(9347..10345)
                     /locus_tag="VFZ86_14240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012949307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX5643477.1"
                     /translation="MPPITDTSTPSAARRDQELAETARRAQRVADNIASVILGKEDVI
                     ESCVVALLAGGHVIVEDYPGVGKTLLAKALARSVGGRFSRIQFTPDLLPSDVTGVNVF
                     DQQTARFEFHPGPVFANVVLADEINRASPKTQSSLLECMEEGQATIDNVAHRIVLPFM
                     IVATQNPIEYEGTYPLPEAQLDRFMMRLSLGYPSAAAEEAILESQTSGDPFALLEPVV
                     EATEVLAMQEAVLRVQVAPALRRYVVDLVAATRESREVYLGASPRAGIALLRAGKAHA
                     VLRGRDYVVPQDVKDLAVRVLGHRIILSAEAGAHAQAEEAVIARILEAVPVPGPGR"
     gene            complement(10469..11575)
                     /locus_tag="VFZ86_14245"
     CDS             complement(10469..11575)
                     /locus_tag="VFZ86_14245"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HEX5643478.1"
                     /translation="MKRWTDLMLVACAAGVALAQWFAPVAVEAVLGGLLFCLVVLTVV
                     SRRAAVGQTLESLTAAHREQLESERRYRALFDACSDVILVYRFEDDGSPGQIVEVNEA
                     ACLALGYARAQLLAMTADDVLAPEARKQVRDRAQALADAGTLAYETVHITSDRQRTPV
                     EVTARIVDIGGRRLCLTVSHSIAAHKELEEFLRGLTDVDELTGLLNRRGFFVKVDDMV
                     RRARRGDRQVLLMYFDLDGLKQVNDEQGHAEGDRLLVAAADVLRAAFRERDVVARIGG
                     DEFVAMALLGRRHDEMLDRQAIEARLDEAMRAKRAELGDCYDFSMSSGAMVANHVQLG
                     GIDELLARADQEMYTAKRARRRDERPAKVSQQRS"
     gene            complement(11746..12387)
                     /locus_tag="VFZ86_14250"
     CDS             complement(11746..12387)
                     /locus_tag="VFZ86_14250"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX5643479.1"
                     /translation="MSLAYRVLLPPAGSRRRRPGDAAAAGVGAAGLATTALVAAAGQT
                     PALEREVFAAVNRLPGAAAPGVWVVMQAGSLPAVFVAGGLALAARRPRLALGLVAGGT
                     TAWALAKVVKQAVGRGRPEALLHDVIVHGTAATGLGFPSGHAAVAAALVTIAAPYVTR
                     PARIAGWAVVGLVAASRVYVGAHLPLDAAGGIFLGWVVGSGVGLVLGVPARRA"
     gene            complement(12384..>13196)
                     /locus_tag="VFZ86_14255"
     CDS             complement(12384..>13196)
                     /locus_tag="VFZ86_14255"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEX5643480.1"
                     /translation="WALWAVVASAATYVAAAIGLMGAVPGHVPFFGTVGAQLAGSFVN
                     RITPARVGGIATNVRFLQKQGIDLAVASSSIGLQQLSGLLVHVSLSFLFLLWAGRSGA
                     GAIDFLPSGQTVLVGLTAVLALSGLLFLLPWGRKILSKRVLPILRRSGHGIADVARRP
                     LKLVELFGGSAVLTLAYIAAFVLSVEAFGGGVGIASIGVVYLVGSAVSSVAPTPGGIG
                     AVEAALIAGLTAVGLEREIAVPAVFLFRIATFWLPIVPGWIMFFVLQRRGDL"
BASE COUNT         1850 a         4785 c         4943 g         1618 t
ORIGIN      
        1 cgcacgtcgg gctcgccggc cgcggccgta ccgcggcggc cggcgagcag cccgtgcgcg
       61 gcgagcaccg cgagcgcggc gatgatgagc accatgccga tgccctcgac gagcgtcggc
      121 acctctccca gctggatcca cgcggcggcc acgccgacga ccgggatgga cagcgtcccc
      181 agtccggcgg cgccggccgg cagtgcatgc agcgcgtaca gccagaggaa ccaggcgacg
      241 gcgttggcga gcacgaccgt gtagatgagc ccccaccaga agctgagcgt ccactcgggc
      301 ccgccgctgt aggtgagcag ggcggcgacg atcagcggca cggagccgaa ggccatctgc
      361 caggtggtga gcgagaggac gtcgacgcgg tggcggcgct gcatgagctt gaccgcgagt
      421 gcgctcgcgg cccacgagag cccgccgagc agggtcagga tgccgctggc ggcgcccgcg
      481 agctcccatg ggcgcacgac caggacgagc ccggcgaagg cgagcgcgac ggcgagccac
      541 tgcacgccgc gcaggcgctc gccgaggaag acccaggcga gcagcagcag ccagaacggc
      601 atcgtgtagg tgatcacgga gacccggccc gcgccggtgt cgtggagcgc ggtgatgacg
      661 aagccgacga acagcgtggt ctgcaggagg ccgatgagca tcgtccagcc gagcggcggc
      721 gggcgcagcg gcttgcgcag cacgagcatc acgaggaaga ggcaggcggc gctcagcgtc
      781 acgcgcagcg ccgcgaacgt cagcgcgtcc gagtactcga gggcgatctt ggtggccacc
      841 cagccgtagc cccagatcgg gccgaggacg aggagcgcga tgatgccgag atcgcgggtg
      901 cggtgtgcgg aggcgggggc catcagcctc atgctagcgg acgcgggacc cgtcgcgtcg
      961 cggtggccgg ccggtcgcgc gacccgtcac acgggtcgtg tcagtcgatg atcggaagga
     1021 gggccggctc ctcgccgccg gccggcccgc cgccgcgtcc cgccttgacg cccgtcgccg
     1081 ccagcactgc cagcgcggcg atgatgagcg ccatcccgac cccctcgacg accgccggcg
     1141 cctctccgag ctgcagccag gcggtgacca cgccgacgac cgggaccgcg agcgtgccca
     1201 tcccggccgc gccggccggg agggcgcgca gcgcgaagat ccagagcgcc cagcaggcgg
     1261 cgttggcgag caggatggag tacgcgaggc acgcgacgaa gacgcccgtc cactcgggcg
     1321 ccgtgccttc gaccagcacg gcgacggcga tgagcggcac ggagccgagc gccagctgcc
     1381 aggcggtcag cgagagcgtg tcgcagcgcg tgcgccgctg caacagcttg accaggagcg
     1441 ccgacccggc ccacgcgagc cccgccccgc acgcgaggac gccgctgagc gccccgccga
     1501 tatcccacgg gcgcacgacg aggacgaggc cggtgaaggc gagcccgacg gccggccact
     1561 ggaggccgcg caggcgctcg ccgaggaaca gccaggcgag cgtcagcagc cagaacggca
     1621 tcgtgtagac gagcaccgac accttgccgg cgccgcccac ggtcagcgcg gcggtggcca
     1681 tgccgttgaa cagcgccgtc tgcaggaggc cgatgagcgc cgtccagccg agcggcggcg
     1741 gccgcagcgg ccgccgcgtg acgacgagga cgacgaacag gcacagggcg cccagggagg
     1801 agatgagcgc cgcgaacgtg aacggctcgg agtactcgag cgcgaccttg ctgacgaccc
     1861 agctgtagcc ccagatcggg gcgatgaccg cgagcgcgag gatgccgagg tcgcgggtgc
     1921 gcttggagga ggtcgcaggc atgcggccat gctaacggac gacgccgggg gccgcagagg
     1981 accgggtccg gcgcaggaag tcggccgcgg cgcgcgaggc aggaggcccg ggcccgcgcc
     2041 gcgggagtca ggccgtcgcg atcgccgccg ggacgtcgct ggtctcggcg agcagcctga
     2101 ggttttccat gctgtcttcg aagtcgcggc gcatgcgctt ctcgaacagg agccggtcgg
     2161 ccgcccggcc cccgaaacgc cgcgggacgt cgtagtcgaa ggtggtcgtc acgtcggtgc
     2221 cgtcggcgcg gggctcgtgg tggcacgaga tcgtgcccga catcgccccg tcgatctgcc
     2281 agcgccagtc gctgctgccg tccgggttgt gccgctcctc gacggtgcgg tccaccatgc
     2341 ggaccttcac ccccatcatg tgctggaaga actccgcttt ggtgcccgga ctgccgtcgc
     2401 cgaacaccct cggcgacgcg tccatgccca cccagaacgt gggccaccgg cgcgggtcct
     2461 tgacgacccc gtcgaagacc tcgacgggca cgccgacgtg gaagtcgttc gtcagatgtg
     2521 ccatagcgcc tccccctcgt ggctgagctc cgcgcacccg cgcggatccg ggcctcctca
     2581 atcgacggtt cccgtggcat ggtgagccca acaccgttga cgacgatcca tgcgagacgg
     2641 cgatggccgg ggcggcgcat cgttccgcac tccgagtggg tacaaagcct gcgtcggcca
     2701 cgccggggcg gtgctgggag cgacaggagg aagaggacat gatcgacgcg atcaccctcg
     2761 gggcggggcg gcaggagacc gggtggacgt cctctccggc ctgacactgg ccgcgccggc
     2821 cggtctcaac gcgtacatcc ccctgctcgc ggtggcgctc gccgagcgcc tcggttgggt
     2881 gcaccttcga gagcccttcg acgggatcgg ctcgtggtgg gtcatcgggg tgatcgccgt
     2941 gctgctcgtg gtggaggtgg tggccgacaa ggtgccggcg gtggaccacg tgaacgacgt
     3001 gatccagacg gtggtccggc cggccgccgg cggcctgctg gcggtctcgg cctcgacgca
     3061 gggcacggtc gagccgctcc tgctcctcat cgccggggtg ctcatcgccg gcggagtgca
     3121 cgccgtgaag gccaccgccc gcccggccgt gaacgtgacg acggccggcg tcggcgcccc
     3181 ggtggtctcg accggcgagg acatcctcgc cgtgatcggc accgtgctcg ccttgctggc
     3241 gccggtgctc gcggcggtgc tgatcgtcgc cgcggtactc ctcgcggtgg tgctcctgcg
     3301 ccgccgccgt cagcgcgccg cgtagcgccg gcggtcggtt ccggcaccca tgagcagcgc
     3361 caggcccacc agatccttcg gctggtacgt ggtgggcgcc tccttcgtcg tcctgttcct
     3421 gaacgccggt gcgcggctga tgatcggcgt gctgttcaag cccgtcatcg cggacttcgg
     3481 ctggagccga tccgcggtgt cggcggcggt cctcgtgaac ctggccgtgt tcgccgtggc
     3541 gatcgtcgtc gccggacggc tgttcgaccg gtacggcccc aggtgggtca tcctcgtctc
     3601 cagcgtcctc ttctcgggtg gactgatgct gacggccgtg atgacggacc tgtggcagtt
     3661 cttcctgttc tacggcgtca tctcggcggc aggcctcggt ggcacctccg cgccgatctt
     3721 cggagccatc gtcagccgct ggttcgagcg cggccggggc accgccgtca gcctggccat
     3781 ggcggggacg tcgctcggcc agttcgcgct ggtgccgctg ttcaccctct tcctcatgga
     3841 ccagggctgg cgggccacgg tgttctgggt gggcgtgctg tgcctcgtgg tgaacgtggt
     3901 cctcgtgttc gcggtgctga gcggcgacca cggtgatccc ggcaaggctc gcgaccgaca
     3961 cgaggcctcc gcgcgcgccg ccgccacgcc ggcggccccg gcggaggcgg gagtcgccgt
     4021 cccccgctcg ctgaccctgc gagaggccat gcgcacggcg tcgttctggt acttcaccgt
     4081 cgccatgttc atctgcggcg ccggcgactt cctcctcacc acgcacctcg tgccgatggc
     4141 gacggaccac ggcgtggcca cctccaccgc cgtggccatg ctggcctggt tcggcctgct
     4201 cagcctgggc gggatcctgc tcgcgggtcc cgcctccgac ctcatcggcg accgcgtccc
     4261 catcgccatc gcgttcggcc tgcgcgtcgc cctcttcctg ctggtgttcc gctatcagaa
     4321 cgcggcctcg ttctgggcgc tggcgctggg cttcgggttc accttcctgg tcacggcgcc
     4381 gctgacgacc accctcatgg ggcgcctcta cggcttcgcc cacataggcc tcctcggcgg
     4441 cttcatcacc accgtccacc acgtcggcgg cggcatctgg gcgtacctcg gcggcgccgt
     4501 gtacgacgcg accggcggct acacgccggc gatggcgatc tcggccgtcg cgtcggccat
     4561 cgcgctggtg ctgacgctgc tgatcaagga ggtgcggcac gtcgcgccgg aggagccgca
     4621 cgggcaggat tccgcgccgg ccggggaacc ccggttcgcc ggcgcggcca cgggcgcccg
     4681 cgtagtcacg cgcacgcggg gtatgtgagg agcacaaggt ccagcgcaac gctacggcgg
     4741 gagcggcagt ccatgagcga catcgacgac cacgtcattc cctacgagga agccgtccgg
     4801 cggcggcgcg cccgcgaggc gcagaaggag agaagcgccg ccgctggact gaagatcagc
     4861 gacgccatga gccacctgct cgagcaggcg ggcacgcagg acgacgaggg gcacatcccc
     4921 gcccacctgc gcgacatcgt cgagcgcgag ttcgcggacg gggagccgcc gccggaccga
     4981 ccgtccgccg ggcgtcctgg aggcgatgcc taagttttcg cgctcctgag cgtcttgatg
     5041 ggtgaagagg cgatcacgcc atcgttgcga ctcaggaggt cgatgtgagg ctcatcaaag
     5101 tgctcgtgct cgtgggggtg gtcctgctgg tggggagcag cgtcgcgctg gccggcgacc
     5161 ggctcgcgca gcggacgcag gaccgtgacc ggacgccggt cgcggccaag acctgcacgc
     5221 ctgcgccgga cgcgacctgc gcggccgtcc cggctcggga ccgcgaccgt gaccgtgacg
     5281 ggtcggccgg cgagcagacc gccacgggcg accaggtgcg cgaccgcgac cgcaccggag
     5341 actgcgacgg gaccggcgac ggtcagcagg cgcagacgcg gcagcgcgac ggccagcaga
     5401 cccagacgca gacgcagacc cagacgcagt cgcagaagca gacccagacc cagacgcaga
     5461 agcagaccgg cgaccaggcc agagtcggtc agcccgcggg cgcgggtacg gggaacggct
     5521 cctgcgacgg gtccggcagc ggctccagca acggcaacgg cgccggcgat ggtaccggcc
     5581 ccgtcgccgg ctccggcgag tgcgacggct caggcgccgg cgcggggaac ggcgccggtc
     5641 actgacgggc gcctgacgga gggcgccggt caccgaccgg tcgcccgacg gatcacgagg
     5701 cggcgggctg ccggcggcgg cccgccgcct cgtcctcgtc ttcagggcgc cgcgatcgtc
     5761 ggcccgcggc gcgtctccgt ctcaggaacg cgtggcgggc cgggcgcgcc gggcgacggg
     5821 caggcgcgac ccgcggcgtc gccgggggcg cacgccgtag agcgcgagca gggccgtgag
     5881 ccggccgctc cgctcgcgga gccgtctcct cacgcggcgg cgcagggcgc cgaggtcctt
     5941 caggtccgca tcgctcaccc ggcggctgcc gaactccacc gcctggacgc ggtccggcag
     6001 gtccccggcg tccaccccga ggtggtcgct gaggtagcgc gccgtctcgt cgagggtctg
     6061 cgacggcttc gcctcggcgc cgtggtcacg gaggtccgcg tacaggaggg cgacggacgc
     6121 gcggagccgg tcctcccggg agccgcggcg caggccgcgc ccgcgcagca gcgcgcggcc
     6181 cgccggccag gcgacggccg cggccagcag cgacaccacc agccagggcg cccggccgcc
     6241 gggctcctcg gcgacgacgg tctcgtccac ctgcctgctc ggatcggcca cgcgcgcccg
     6301 gccgtccacc gcggccgcct ccggggccgg cgacgcgccc gcgccgtcca gcccggccgc
     6361 agccgcggca tcggggccgc tggcgggggc cgtgctcgtg ctcgggatct cccggccggg
     6421 cgtcgggtcg aactgcgccc accccacgcc ggggaagtac gcctccaccc acgcgtgggc
     6481 gtcgttgcgc gtgaccaccc agatgccgtt gcgctcctcc tctccggcgg tgaagccgac
     6541 cgccaccctc gcggggatgc cgttgaagcg caggatcgcg gccatcgccc cggcgaagtg
     6601 ctggcagtac ccgatacgcg tggcgaagag gaactcggcg tacggcgagt cgaagcccgc
     6661 atccggcggc cgcagtgagt agtcatggtt ggagcgcagg tactgctcca cggcgagcgc
     6721 tacgcggaac gggtctgtct cgcctcccac gaccctctcg ttgagcgcgt agagccctag
     6781 ccactcgcgg ccggcgggga cggcggccgc ctcggcccgc cacgcgctct cagaggacgg
     6841 gccgccggcc gccggccccg ccgggaacgg cagcgccgtg aagctgcgcg tgacgtctcc
     6901 ggggtagtac cgcccgcgcc cgacgaggtc cgtcggctcg aggtccggga ccaccgcggc
     6961 gacgctgtag tccagggagg ggccgcgggg cggctcgacg gcgaccgccg ccgcgctcgt
     7021 catctgcagg tcgagcggca gcgcggagcg cacctcgacc gcccagcctc ccacgaacag
     7081 gcggtcggtg taggcgccct cgatctcgaa gcgctgcgtc acgagccttc cctgcggcgc
     7141 ggggcggatc tgcggggcct cgtagaccca gtccccgccg cgcgcctggg gccgcagctc
     7201 gcggccgtcc ggcgcaccgc cgcgccaggt cgtcccggtg aagtccgcga ggacgctcgc
     7261 ccgccagtag gacgcgatgg gcgagcgcac gcgcatgacc acgacgtcgt tggcgggttc
     7321 gaggagtctg gggtagttct gcatgcggtc aaagcggaac ttggccgtgc gcgggcccgt
     7381 gatgtcccac gagcgccagt cgtgcagggg gcggcccgcc tccaccgccg tcgtaccgat
     7441 gatggacagc gcgaggagtg ccgcgagcac ggcggtcacc ccgccggcga gcgcgtcggt
     7501 ggcgtgccgg cgctgctgcc cgagcgagcg cgaaagggag agcatccagc cggcgaagag
     7561 gacgaaggcg atggcggccc agggatcgcg tgccgacgag tcggtcgtga acccgaagcc
     7621 cgcgagggcg aggagcatga cgacggcggg gagcggccgg cgcaggctca aggcaaggaa
     7681 cgccgcgacc ccgaccgtga cgtagacgac cagcgacagc agcaggcgca gtcctgcctg
     7741 cgacgagacg tcgagcggga acacgtcgta cgcgtacgcg atgccgccgg cgcccagctg
     7801 gtccgcgtag aaggcgaggt gagccgggac cccgctcgcc tgcggcggca gcggcacctg
     7861 cgcccgcatg aggaggtagg cgccgagcgg cagcaggaga agggcgagcg cccaggcgcg
     7921 ccggcgcacc agtccgggcg cgccggcgag cgtcgcgacc gccgccgtcg ccagcagcag
     7981 cggcgcgatc gacgggtcgg tgacgcggcc ggcgacgacc gcgctgacgg cggcgaggcc
     8041 ggcgaagccc acgacgtaga agagcgcccg ccgtccgttc atcgcgccct cgccagtcgc
     8101 tccggggcgc cggcgctgag ggccgagccc aggtcgtcgt cgcgatgcag ccgcacgtag
     8161 cgcactccgg ctgcgacgag ggcctgccgc atctccgcgt ccgtcgccgc gccgggcgcg
     8221 gagcggccat cgctcgcgtc gccgcccgtc acctctccgg tgacgtgcac cacggagacc
     8281 gggacgccct gtgtgcgcag ccggcgcagg gcgtccacca ggccgccgtc caggcacagg
     8341 acgacgagtg cgaggacgcc gcggcgcacc tgcgtccggc cgccgacgat ggcctggagc
     8401 gacgggccga gccgggaagg cccctgaggc tcggcctccg ccagcacgcg cagcatccgc
     8461 cgccggctgg cggcgtccgg cgtgaggcgg atgggcctcc agtcgtgctc gggaagcagc
     8521 agggtgaccc cgtgtccggt gcggagggtg aacgccgcca tcgagccggc ggcgcggacc
     8581 gccgcctcga aggcgctgtc cggcgcctct ccggcgtcgc acgccgccgc gccgctcagc
     8641 agcaccgtca ggtcgtcttc cgaggcagcc tccatctcgc gcagcatgag gctgcccgtc
     8701 ttggcggtgg acttccagtc cacgcggttc agcggctcgc cggggacgtg gggccggacg
     8761 ccgtggagct cccatgcgtc ccgcgtgggc agccgccgcc ggccgccgcg gttccgcacg
     8821 ccgccggcgg ccctgcaggg agcgcacgag gtcagttcgt cgagccgggg cgccaccgtg
     8881 aggcgcagag gctcgccggc cgggcgtcgc acgcggacca ggccgagctg gtcctccacg
     8941 acggcggtga agggcgggag ccggtggacg ccccgccggg ccggccacgg tccggcggtc
     9001 accgtccgct caccgtgcgt tcccggctca tcgaccacga ccggcccgtg gctcccgagc
     9061 gcgcccgtgg cgccctcgag cgtgacgtgg aggccggtca gccggcggcc gctgcgcacg
     9121 tggaagaaga acttcaaggg gtcgccggcg acgggtccct ccggcgcgac gcgtctgtcg
     9181 accaggagcc ggcgcgaacc gctgtgcacg aggagccagg cgacgacggc catcgcggcg
     9241 agcgcgagcg cgatcaggta cagctcccag gtgccgacca cgcgcgccgc gacgtaggtg
     9301 gcggcggcga ccgcgagcag ggcgactccc cgtgccgtcg gccgcgtcat cgcccgggcc
     9361 ccggcacggg gactgcctcg aggatgcggg cgatcaccgc ctcctcggcc tgtgcgtgcg
     9421 cgcccgcctc ggccgacagg atgatgcgat ggccgagcac gcgcaccgcg aggtccttga
     9481 cgtcctgcgg caccacgtag tccctgccgc gcaggaccgc gtgcgccttg ccggcgcgca
     9541 ggagggcgat gccggcgcgg gggctcgcgc cgaggtagac ctccctgctc tcgcgcgtcg
     9601 ccgccacgag gtcgaccacg tagcggcgca acgccggtgc gacctgcacg cgcagcacgg
     9661 cctcctgcat ggcgagcacc tcggtggcct ccaccacggg ctcgaggagc gcgaacgggt
     9721 cgccggacgt ctgcgactcc aggatcgcct cctcggccgc ggccgaaggg tatccgaggc
     9781 tgaggcgcat catgaagcgg tcgagctgcg cctcgggcag cgggtacgtg ccctcgtact
     9841 cgatcgggtt ctgcgtggcg acgatcatga agggcagcac gatccggtgg gcgacgttgt
     9901 cgatggtcgc ctgtccctcc tccatgcact cgagcaggct tgactgcgtc ttgggtgagg
     9961 cccggttgat ctcgtccgcc agcacgacgt tggcgaacac cggaccgggg tggaactcga
    10021 accgcgcggt ctgctggtcg aacacgttga cgccggtgac gtcgctgggc agcaggtcgg
    10081 gcgtgaactg gatgcgcgag aagcgcccgc ccaccgagcg cgccagcgcc ttggcgagca
    10141 gggtcttgcc gacgccggga tagtcctcga cgatcacgtg cccgccggcg agcagcgcga
    10201 cgacgcacga ttcgatgacg tcctccttgc cgaggatgac ggaggcgatg ttgtcggcga
    10261 cacgctgtgc ccgccgggca gtctccgcga gctcctggtc ccgtcgcgcc gcgctggggg
    10321 tgctggtgtc tgtgatgggt ggcatggtcc ctcgctggtc ggtcacagct acgtcattcc
    10381 ccatttgggc ccggggacgt cgcgggatca cgggggaggg cagtgcggat ggcgagggaa
    10441 cgccgtggga cgccggtccc ggccgcgctc aggaacgctg ctgcgacacc ttcgcggggc
    10501 gctcgtcgcg gcggcgcgcg cgcttggcgg tgtacatctc ctggtcggcg cgggccagca
    10561 gttcgtcgat gccccccagc tgcacgtggt tggccaccat ggcgccggaa ctcatcgaga
    10621 agtcgtagca gtcgccgagc tccgcgcgct tggcgcgcat cgcctcgtcc aggcgcgcct
    10681 cgatcgcctg gcggtccagc atctcgtcgt gccgccggcc gagcagggcc atcgccacga
    10741 actcgtcgcc gccgatgcgg gccaccacgt cgcgctcgcg gaaggcggcg cggagcacgt
    10801 cggcggccgc cacgagcagc cggtcgccct ccgcgtgtcc ctgctcgtcg ttgacctgct
    10861 tgaggccgtc gaggtcgaag tacatgagca gcacctgacg gtcgccgcgt ctcgcccggc
    10921 gcaccatgtc gtccactttg acgaagaagc cgcgccggtt gaggaggccg gtgagctcgt
    10981 cgacgtccgt gaggccgcgc aggaactcct ccagctcctt gtgcgcggcg atgctgtggc
    11041 tcaccgtgag gcagagccgg cggccgccga tgtccacgat ccgcgcggtg acctcgaccg
    11101 gcgtgcgctg ccggtcactc gtgatgtgca cggtctcgta ggcgagcgtg ccggcgtccg
    11161 ccagggcctg tgcgcggtcc cggacctgct tgcgcgcctc gggagcgagg acatcatccg
    11221 ccgtcatcgc caggagctgg gcgcgcgcgt agcccagcgc caggcaggcc gcctcgttga
    11281 cctcgacgat ctgccccgga ctcccgtcgt cctcgaagcg gtacacgagg atgacgtcgc
    11341 tgcaggcgtc gaagagggcc ctgtagcgcc gctcgctctc gagctgctcc ctgtgcgcgg
    11401 cggtgagcga ctcgagggtc tggccgaccg cggcccggcg cgagacgacg gtgaggacga
    11461 cgaggcagaa gagcaggccg ccgagcacgg cctcgacggc gacgggcgcg aaccactgcg
    11521 cgagggcgac gcccgccgcg caggcgacga gcatgaggtc cgtccaccgc ttcatcggcc
    11581 ggctctccgc tgcatcgcct gtccgggctg ctgcccattc cgcacgctca cccggtgctc
    11641 cctgtcgcgg tggcccgtgt ccgggctctc agcacagggc atcggcggcg cgcggcgagt
    11701 cgacaccccc ctctagacac atgtacttgc cggtcggcgg tgcgctcagg cgcggcgcgc
    11761 cggcacgccg agcacgagac cgacgccgct gccgacgacc cagccgagga agatgccccc
    11821 ggcggcgtcg agcggcaggt gtgcgccgac gtagacgcgc gaggcggcga ccagcccgac
    11881 gaccgcccag ccggcgatcc gcgccggcct ggtcacgtag ggagcggcga tggtcacgag
    11941 cgccgctgcg acggcggcgt ggcccgacgg gaaccccaag cctgtggcgg cggtgccgtg
    12001 gacgatgacg tcgtggagca gcgcctccgg ccggccgcgc ccgacagcct gcttgacgac
    12061 cttggccagc gcccaggcgg tcgtgccgcc cgccacgagc ccgagagcca gacgagggcg
    12121 tctggcagcc agagcgaggc ctccggcgac gaagacggcg gggaggctgc cggcctgcat
    12181 gaccacccag acgcccggcg ccgccgcgcc cggcaggcgg ttgacggccg cgaagacctc
    12241 ccgctccagt gcgggcgtct gcccggccgc cgcgaccagg gcggtcgtcg cgagcccggc
    12301 ggccccgacg ccggcggccg cggcgtcgcc cggcctgcgc cgccggctgc cggccggcgg
    12361 caggagcacc cgatatgcga ggctcacagg tcgcctcgtc gctgcaggac gaagaacatg
    12421 atccagccgg ggacgatcgg cagccagaag gtggcgatgc ggaagaggaa cacggccggg
    12481 acggcgatct cgcgttccag gccgaccgcc gtcaggccgg cgatgagcgc cgcctcgacg
    12541 gccccgatgc cgcccggcgt cggggcgacc gacgagacgg cggagccgac gagatagacc
    12601 acgccgatgc tcgcgatgcc gacgccgccg ccgaaggcct ccaccgagag gacgaacgcg
    12661 gcgatgtacg ccagcgtcag gacggccgat ccgccgaaca gctcgaccag cttgagcggc
    12721 cgccgggcca catcggcgat gccgtgtcct gagcgccgca ggatcggcag cacacgcttg
    12781 ctcaggatct tgcgtcccca cggcagcagg aacagcaggc cggagagcgc gagcacggcc
    12841 gtcagcccga cgagcacggt ctggccggag gggaggaagt cgatcgctcc ggccccgctg
    12901 cggccggccc acaacaggaa gaggaacgac agcgagacgt gcacgagcag gcccgagagc
    12961 tgctggaggc cgatgctcga cgaggcgacc gcgaggtcga tgccctgctt ctggaggaag
    13021 cggacgttgg tggcgatccc gcccacgcgc gccggcgtga tgcggttcac gaacgagccc
    13081 gcgagctggg cgccgaccgt gccgaagaag ggcacgtgcc ccggcacggc gcccatgagc
    13141 ccgatcgccg cggcgacgta cgtggcggcc gacgcgacca ctgcccacag cgccca
//
LOCUS       DASUMX010000303        15203 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_546946, whole genome shotgun sequence.
ACCESSION   DASUMX010000303 DASUMX010000000
VERSION     DASUMX010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 15203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15203
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546946"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..1593
                     /locus_tag="VFZ86_14260"
     CDS             157..1593
                     /locus_tag="VFZ86_14260"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HEX5643481.1"
                     /translation="MSFGAELPEAASVAAVHESALRVLEMTGVLVQDDEAVELLRARG
                     ARCDGRRVWLRADLVQQALATAPSSFVLAGRRPELGLPLGGADGRVFGSGSGAAYMVE
                     SQPAVPDAAGPAMRPGTLADLVATAKLGHQLPALDFNSDCMEPLDLPEDVRTRRGTHA
                     RLTLSDKAIEWVASVDEDLDDAERLNEILFGASWHHRPRALIVLNTSTPLQLSGETAR
                     ILLRWARLGQPTCVTACVMGGTTGPATLAGVLVVQHAEVLAALVLSQAAQEGSPFVYG
                     GVSAMASLRTGAALFGTPEFARLARATVGLAHRCGLPVRAGAAVTDAHVVDARAMAES
                     AAGLSAAAFAGGHFLFQATGMLSSFNALSLEKYVLDADVAVSLRAALHDVPVGDDELA
                     EATIDAVGPAGGYLGEAHTRRHARDCERPEPAAPPAPEPFERWAAAGGRDALATAARR
                     VKELLAAHEPPDDLDAVTRRQLDEYCLA"
     gene            1846..3441
                     /locus_tag="VFZ86_14265"
     CDS             1846..3441
                     /locus_tag="VFZ86_14265"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5643482.1"
                     /translation="MSEKSTYRSRRGAMGTMLVLAIVLALAFAIVGCGGTESDEGESP
                     SAAGAPTTGGTLNVGMQPGNGQYDPVMMAGSVGDILLVCQVQENLVGLAQDFSVKPWL
                     AKSWDSPDGKSWTFMLQEGVTFSNGEPFTSEDVIYSFERLRSKESPMAGVYANIEDVV
                     ADSPTQVTFKLKAVDSEFPSSVTDYRAKMLCKSVADPMTELVGTGPFMLESFAAEDRA
                     VLVKNPTYWQKDESGQQLPYLDGVEFIYSPDTAGQIEGLQGGALNWVGGLTAEQKQTV
                     EANPSLKVITTNTNYMNALQIRVDQGAGKDLKVRQALWAGTDRQAIIDLVAPGVGVAG
                     NGTFVGPAYEAYFTDEQPAYDPEAAKQLLADAGYADGLEIKLVAQTADPIPAVATAWQ
                     AQMKEIGVDVKIQQVPTDVFYADKGTDNWYQADFGIVDWGTRASPITYFKLALTSDAP
                     WNYSRWKNPEFDKIVEEIPLALDDAERADLYKQAQAILQDQVPVMNFIILTAVAGESA
                     NVDGIELDPDWAHTTFTHAYFTE"
     gene            3687..4910
                     /locus_tag="VFZ86_14270"
     CDS             3687..4910
                     /locus_tag="VFZ86_14270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643483.1"
                     /translation="MIVADERGGRRAAARRRVPIGPVEFALDPAAVEGARELTRRAWR
                     YREVERVPVIVDLGPECDESVHDVLLEDEAWFNSGVRRIERSLRLLQDDYIPVFEPPW
                     AGYFSTPAMLGAELWWEEDPDSWPAVKSPPIRDLSALRGLEPADVASSPHFSRILTRL
                     GIARDCLPPAVAIGGVDMMSPLGDLQGIMDQTLMFLSMKQNPDALRRACDVITSTQEA
                     VQDATLEAVGGEGRLAGLTNWPIWRPEGAKVLVTDDVAGLLSPAVYEAFDKPYGDRLL
                     RRYGGGLRHVCGPHPALSLYMSEDPLVHGLNCAYRFSREHLPALREEMGPRAREACGR
                     RGHLEVMFERDMPLPGIVEAFRCLADALAPDVVAIPYCQVASDGSVSDDEIAAFGVEM
                     RRVAEEYAARIHWDG"
     gene            4928..6220
                     /locus_tag="VFZ86_14275"
     CDS             4928..6220
                     /locus_tag="VFZ86_14275"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5643484.1"
                     /translation="MHVVIIGNSAAGLSALDSLRRRDRAATVTVVSSEGARPYSRVLL
                     PYYLRGRIPHAGLFIRPEDHYERLDARTALGEVVERVDAEEHRVVLASGRSIGYDRLL
                     VATGSRPGAPPIEGLAGPGVQGFWTLEDAERLDRDLRPGARVMVLGAGFVALQAAWAA
                     HRRGASVTVVELEGRILPRVLDEEAARLLRAHIERTGVRIATSVCISGVERACGELCC
                     TAAGTDTYEADIVIVATGVLPNDGLLPEAVSAQGPGLEVATTMETVVADVFAAGDVAR
                     APMWGGAVGVLALWPVAIAQGRVAGANLAGAGLEYEGGLSANVTEMFGVTVASAGRFE
                     EVPGEEVVVLRDLPGVEYLKLVLTGGVPVGAVYLGGQQAVRLLGRLGPYIRHGRPLGD
                     PRAFFEERRSTGMVAVTPWFAHRAPDLGSAERTVTCAS"
     gene            6208..6693
                     /locus_tag="VFZ86_14280"
     CDS             6208..6693
                     /locus_tag="VFZ86_14280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008717822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX5643485.1"
                     /translation="MRIVVGDPEYCVACRDCAYACAFAHGGGFDRESSRIAVSFSPED
                     RVCLPLTCMHCAEAWCMEVCPADAISRDLETGAVVIHEERCAGCKMCLLACPLGAVGF
                     DAAAGVARKCDLCDGDPACVRSCTVDVLQYCEPGEMADSRRESLEARLRAVYRKGEGG
                     S"
     gene            6693..8741
                     /locus_tag="VFZ86_14285"
     CDS             6693..8741
                     /locus_tag="VFZ86_14285"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX5643486.1"
                     /translation="MEAPPQTVQTLCRMCDDRCGIDVRLEDGRVVDITGNPAHPWNLG
                     RLCVKARAAVDTVNHPDRLLHPLKRHGDDWEEIPLEQALDEIAERLRAIIEKDGARSV
                     SVWKGEAIGFAQQEDLARRFIHAIGSPNYLSNDSMCWVGRFTGYKLVLGAWPNVDIEN
                     ARCVVMWGANPPHSRPNLTQRITKARRRGATLVVVDPRLSAVARRADLHVAPLPGTDG
                     ALALGLFHELVESGSYDRSLVERTTVGFDDLAGYAREFTPDVVERETGIPPATLSEMA
                     RLLRAADGRVALYPGNGLEHHENGVDNIRAILSLNALLGSLGKVGGSLFAPPLPLRDL
                     TLYDERPLTELRPLGADRFPVLYEVRRECHTMTALNGILTGAPYPLRAMLLAGANPAL
                     TNPNSHRVVEALTKLDLLVVRDLFMTETAALADYVLPAASFLERTEVHTHHEIGVVTL
                     TAGVGRHPEVQTEYEFWRDMAHRLGAAEYFPWEDDRELNRWLLEPTAISLEELEAHPE
                     GVTYSSPPADAEIERFPTPSGKVEFVSKHLAELGYDALPKYRRPAYIETPDPAYPFVL
                     MTGARSLLFGHSRSHNISRFAEEAPAPGLEMHPDDASVLGLADGDTARVTTRIGSVEA
                     PVRVVATNELPRHCLQLTHGWAGVNANALTHDDRFDRISGFPLMKSVEARVEPVGRTG
                     "
     gene            8862..9800
                     /locus_tag="VFZ86_14290"
     CDS             8862..9800
                     /locus_tag="VFZ86_14290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015906200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5643487.1"
                     /translation="MVEPNVKDELFDTAIMDEEAVKGFSDPAQQERPRTWRLYWRRLL
                     KRPAALVGTVIFCFFLFLAVFGPWIAPYDYEKQSAELRLATPTLSHPFGADQFGRDIL
                     SRIIVGTRNIFLLGGFGTAIAVVIGTAIGLFSGYVGGSIDEVVMRLLDVLLSFPSLLL
                     ALVLLSTVGPSNINIVFVVAILYIPMVARVVRSMVLDLKNKEFVEAARVRGETRSYVL
                     FREILPNSLPPLLVEASMRFSYSIFLVASLGFLGLGVQPPSPDWGLQINQARAFFAIA
                     PWVLLFPACTIAVLVIATNLMSDGLRQVMLPGGGRN"
     gene            9800..11860
                     /locus_tag="VFZ86_14295"
     CDS             9800..11860
                     /locus_tag="VFZ86_14295"
                     /inference="COORDINATES: protein motif:HMM:NF008453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5643488.1"
                     /translation="MAAPASRPVFEIEGLCVDYTTELGVLHAVRDVSLAVHEHESFGL
                     VGESGSGKSTLAMGAIRYLAQNGRVTDGSVRLNGVELLDLPDRALRALWGAKIGVVYQ
                     SPLSALNPSIVIGKQLAEVARQHLGMDAAAARDRVHEMLTKVAMPDPEAVTKRYPHQL
                     SGGMLQRCVIAMALMTDPSLLIMDEPTTALDVTTQAVVLDLVAELKREFDSAILYITH
                     DLGVITKICDRVGVMYAGEFMEQAELKELFKRPLHPYTLDLLGCVPHFDPTPQKRSLV
                     TIPGSIPRVDQLPPGCIFAPRCGFVEEACTQARPPLVAVQGAHLSACRRWQAVPPPAE
                     YLRAATEAVREVQTECVAREVLVAADDTQVHFKAPKGTVRAVDGVDLEVCVGQTLGVV
                     GESGSGKTTVARAIIGLTPRTSGDIRLRGDSLALSTAKRPRSTLREIQMVFQNPDASL
                     NPTRSVGDAIMRPLALLGGLDRRQAKDKALELLASVSLPASYFDRLPHELSGGEKQRV
                     AIARAFAAEPGIILCDEPISSLDVSVQGSLMNLLMKLQVEKQTSYLFISHDLSAVQHL
                     SDVIAVVYLGHVMEVGDATTVLTPPFHPYTEALLSAVPLADPDVKQKPIRLSGSVPSA
                     MNVPSGCRFHPRCPRCLGDVCREQEPPWRVGEGDHRIYCHIPLDELARLQAETLVLDA
                     EESA"
     gene            11860..12804
                     /locus_tag="VFZ86_14300"
     CDS             11860..12804
                     /locus_tag="VFZ86_14300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015940512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5643489.1"
                     /translation="MARFILRRLGFMVMTVVLASIIIFWATTVLPGDVASQILGRFAT
                     EEAKSNLRTELGLDRPVVVQYGSWLGNYVQGDWGDSPSMNTLVRPIVFERLGNSLMLA
                     VVAFVMYVPLGILLGLLAALRKNTWVDQTISIGSLAFIGLPEFVSGLILIAIFAIQLQ
                     WFPSQSAIPPDTGFIEALPYLILPAITVALTSLAYVVRMTRSSTVDVLQMDYVRTANL
                     KGLPPRKVLFTHVLRNSLLPTVTVVAIGIGWLIGGLIVTESVYGYPGIGRLLLFAIQR
                     RDLVLIQSTALLIVLIYVVANLVADILYAVLNPRIRYG"
     gene            12982..14085
                     /locus_tag="VFZ86_14305"
     CDS             12982..14085
                     /locus_tag="VFZ86_14305"
                     /inference="COORDINATES: protein motif:HMM:NF014381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HEX5643490.1"
                     /translation="MGTSLRLTVCGAGGAGLAIAADNALKGIDVTLFELPSLAEKLEG
                     PIARGGVEVTAGSETTAGKTGFAPLTATSDAAKAVAGADVVMITVPAMYHDVFMDAVA
                     PHLSDGQIVLFNTGYWGSLRQAVRWAGRLPDVSLAESNIMPYICQPKGDAIHIGRFKR
                     HFCVAAFPGERSGSVYDVVRRIYEQYDPAGTVIDTNIAAAGNPPIHATLTIPVAGYYF
                     DRYMGGKFYQDTTVPGARLVEAFDAERQLLATRLGSQLFEDQLSFDRRSYLYEGAGLV
                     EALRSSPHADWYATADYLEQVCSEDIIYAFVPMVRIGEALGIDLPVTRSMIEVMGVML
                     QRDYWTEGLDLAALGLDGLDLDGVRRFVMTGER"
     gene            14094..>15203
                     /locus_tag="VFZ86_14310"
     CDS             14094..>15203
                     /locus_tag="VFZ86_14310"
                     /inference="COORDINATES: protein motif:HMM:NF012447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HEX5643491.1"
                     /translation="MAVVLTGRSLTLDEVVSAARGGEQVVLAEEALTAMAAASALAAG
                     IAERGAPVYGLTTGLGVQKRTRQQRDDSGFSWRQIAESRAGVGPLAPADVVRAAMLVF
                     ANQMAGATTCIRPVLAERLVRALNDGERPEVRARGSIGASDLAQMSDLAAGVFAGMDL
                     VQGEGLALINSSAFGTGSAALALADAARLLEAADVAAALSLEGFAANLSVLHPAIERA
                     RPDPVLAHTLARLRELLHGSYLWDEGAARNLQDPLNFRSAAPVQAAARRALEYAGSVV
                     AIELNAAQGNPLVSVEEGTIRSACLYEVVGVSAALDFVRIAFASMLLAASERSVKLMD
                     TPWSGLPVGLQEPGSPDLGLSILAITSEALGAEVAT"
BASE COUNT         2253 a         5352 c         5220 g         2378 t
ORIGIN      
        1 cgtgcgctgg caggaggagg gcgggctcga cctcaaggag aaagcccgcc gcaaggcgct
       61 caagctgctc gagacgcacg agccggcgcc gctcccacag ggactggcgg cgcgcatcga
      121 cgcgctggtc gacgggttca ctccggaggg atgacgatga gcttcggcgc cgagcttccg
      181 gaagcggctt cggtggccgc cgtccacgag agcgcgctcc gtgtactcga gatgaccggc
      241 gtcctggtcc aggacgatga ggccgtcgag ctgctccgcg ctcgcggggc tcgctgcgac
      301 ggccggcgcg tgtggttgcg cgccgacctc gtgcagcagg cgctggcgac cgcgccgtcg
      361 tccttcgtgc tcgccggtcg ccgtcccgag ctcggcctgc cgctcggcgg cgccgacggc
      421 cgcgtgttcg gctcgggctc cggcgcggct tacatggtcg agtcccagcc ggccgtaccc
      481 gacgcggccg gccccgccat gcgccccggg acactcgccg acctggtcgc caccgccaag
      541 ctcggtcacc agctgccggc cctcgacttc aacagcgact gtatggagcc gctcgacttg
      601 cccgaggacg tgcgcacgcg acgcggcaca cacgcgcggc tgacgctcag cgacaaagcg
      661 atcgagtggg tcgccagtgt ggatgaggat ctggacgacg ccgagcgcct caacgagatc
      721 ctgttcggcg cgagctggca ccatcgtccg cgcgccctca tcgtgctgaa caccagcacg
      781 cctctccagc tgtccggcga gacggcgcgt atcctcctgc gctgggcacg gctggggcag
      841 ccgacctgcg tgacggcgtg tgtgatgggc ggcaccaccg ggccggcgac gctcgccgga
      901 gtgctcgtcg tacagcatgc ggaggtgctg gctgcgctcg tgctctcgca ggcggcgcag
      961 gagggctccc cgttcgtgta cggcggcgtg tcggccatgg cgtcgctgcg caccggagcg
     1021 gcgctcttcg gcacaccgga gttcgcgcgc ctcgcgcgcg cgacggtcgg tctcgcccat
     1081 cgctgcggct tgccggtccg cgccggtgcg gcggtcacgg acgcgcacgt cgtcgacgct
     1141 cgagcgatgg cggaatccgc ggcgggcctc tcggcggccg cgttcgccgg cggccacttc
     1201 ctcttccagg ccaccggcat gctcagctcg ttcaatgcgc tgtccctgga gaagtacgtc
     1261 ctcgacgcgg acgtggcggt ctcgctgcgc gccgcgctgc acgacgtgcc ggtgggcgac
     1321 gacgagctgg ccgaggccac catcgacgcc gtcggtccgg ccggcggata cctgggcgaa
     1381 gcccacacgc ggcggcacgc acgggattgc gagcggcctg agccggcggc gccgccggcg
     1441 cccgagccct tcgagcgttg ggcggcggcg ggcggccgag acgccctcgc caccgccgcc
     1501 cgccgcgtca aggaactcct cgccgcgcac gagccgccgg acgacctcga cgcggtcacc
     1561 cggcgacagc tcgacgagta ctgcctcgcc tgagcaggat cgcccgccgg ttcagagtcg
     1621 cggcgcatcc tcaagagccc ggatccggag cggaagagtc cggagagccg gacagtccgg
     1681 agccgcgctt gacagacgac cggcagaggc tactgtgcct gatacaccga gctaaggcag
     1741 gtggcggtaa gcatgcggcg ctcgtggtcc accaaagacg accgcggcgc aggggggcgt
     1801 gtcaccctgc cacaggacca gcggatcaca agggggagca acgctatgag tgagaagtcc
     1861 acgtacagaa gccgccgcgg cgcgatgggg acgatgcttg tccttgccat cgtcctcgcg
     1921 cttgccttcg ccatcgtggg ttgcgggggc accgagagcg acgaaggcga gagcccgagt
     1981 gctgccggcg cgccgaccac gggcggcacc ctcaacgtag gcatgcagcc gggcaacggc
     2041 cagtacgacc cggtcatgat ggccggctcc gtgggggaca tcctcctcgt ctgccaggtc
     2101 caggagaacc tcgtcggcct ggcgcaggac ttcagcgtca agccgtggct ggccaagagc
     2161 tgggactctc ccgacggcaa gtcgtggacc ttcatgctgc aggaaggcgt caccttcagc
     2221 aacggcgagc ccttcacgtc agaggacgtc atctactcct tcgagcggct ccgcagcaag
     2281 gagtcgccca tggccggcgt gtacgccaat atcgaggacg tcgtggccga cagccccacc
     2341 caggtcacgt tcaagctcaa ggcggtggac tccgagttcc catcgtcggt gacggactat
     2401 cgtgccaaga tgctctgcaa gagcgtcgcg gacccgatga ccgagctcgt gggcacgggc
     2461 cccttcatgc tcgagtcgtt cgcggctgag gaccgcgcgg tcctcgtgaa gaacccgaca
     2521 tactggcaga aggacgagag cgggcagcag ctgccgtacc tcgacggcgt cgagttcatc
     2581 tactcgccgg acaccgccgg ccagatcgag ggcctgcagg gcggcgcgct caactgggtc
     2641 ggcggcctca ccgccgagca gaagcagact gtcgaggcca atccgagcct caaggtcatc
     2701 acgaccaaca cgaactacat gaacgcactg cagatccgcg tcgaccaggg cgccggcaag
     2761 gatctcaagg tccgccaggc cctgtgggcg ggtaccgacc gccaggcgat catcgatctc
     2821 gtggctccgg gcgtcggcgt cgccggcaac ggcacgttcg tcggcccggc ctatgaggcc
     2881 tacttcaccg acgagcagcc cgcgtacgat ccggaggcgg caaagcaact gctcgccgat
     2941 gcgggatacg ccgacggtct cgagatcaag ctggtggccc agacggccga cccgatcccg
     3001 gctgtcgcca ccgcgtggca ggcgcagatg aaggagatcg gcgtcgacgt caagatccag
     3061 caggtcccga ccgacgtgtt ctacgcggac aagggcaccg acaactggta tcaggccgac
     3121 ttcggcatcg tcgactgggg cacgcgcgcc tctccgatca cgtacttcaa gctggcgctc
     3181 accagcgatg caccgtggaa ctattcgcgc tggaagaacc cggagttcga caagatcgtc
     3241 gaagagatcc cgctggccct cgacgacgct gagcgtgccg acctctacaa gcaggcgcag
     3301 gcgatcctgc aggaccaggt cccggtgatg aacttcatca tcctcaccgc cgtggctggg
     3361 gagtccgcga acgtggacgg catcgaactc gatcccgact gggcgcacac gacgttcact
     3421 cacgcgtatt tcaccgagtg acgtcacgct gaatgggccg agaggacgac ctcggccggg
     3481 gtgctgagac gcgaaagggg ccgcaagagc ggcccctttc gcgtccggtg ggtggtgtga
     3541 caagtccggg acgatatggc aactcacagt ggggacagga cgttctctct ccagcattcc
     3601 agacagggcg cggatgaccg ttgaccgctc aagacgaggg cttcatggtg gccgggtgac
     3661 gctgactccc gcagagaagg cacggcgtga tcgtggctga tgagcgtggc ggacgtcgtg
     3721 ccgccgcccg gcggcgcgtg ccgatcggac cggtcgagtt cgcgctggac ccggcggccg
     3781 tcgagggcgc ccgggagctg acgcgccgcg cgtggcggta tcgggaggtc gagcgagtgc
     3841 cggtcatcgt cgacctgggt ccagagtgcg acgaatcggt gcacgacgtc ctgctggagg
     3901 acgaggcatg gttcaactcg ggcgtgaggc gcatcgagcg ttctctccgt ctcctgcagg
     3961 acgactacat cccggtcttc gagccgccct gggccggcta cttctccacc ccggcgatgc
     4021 tcggtgcaga gctgtggtgg gaggaggacc cggactcttg gccggcggtg aagtcgcctc
     4081 ccatccggga cctgagcgct ctccgcgggc ttgaaccagc ggatgtggca tcctcaccgc
     4141 acttctcacg catcctcacg cgactgggta tcgcccgcga ctgcctgcct cccgcggtcg
     4201 ccatcggcgg agtggacatg atgtcgccgc tcggcgacct ccaggggatc atggaccaga
     4261 cgctcatgtt cctctccatg aagcagaacc cggatgctct ccgtcgggcc tgcgacgtga
     4321 tcacgtccac gcaggaggcc gtgcaggacg cgaccctcga ggccgtcggc ggcgaaggtc
     4381 gcctggcggg cctcaccaac tggccgatct ggcggcccga aggcgccaag gtcctggtca
     4441 ccgacgacgt cgcgggcctg ctcagtcctg cagtctacga ggccttcgac aagccctacg
     4501 gtgaccggct gctccgccgg tacggcggcg gactgcgcca cgtgtgcggg ccgcatccgg
     4561 ccctctcgct ctacatgtcc gaggatccgc tggtccacgg tctcaactgc gcctaccggt
     4621 tcagtcgcga gcatctgccg gccttgaggg aggagatggg gccgagggcg cgggaggcgt
     4681 gcggccgtcg cggccacctc gaggtcatgt tcgagcggga catgccgctg cccgggatcg
     4741 tcgaggcgtt ccggtgcctg gccgacgcgc tggctccgga cgtcgtggcg atcccgtact
     4801 gccaggtggc gtccgacggc tccgtgagcg acgatgagat cgcggccttc ggtgtcgaga
     4861 tgcgccgcgt tgccgaggag tacgcggcgc ggatccactg ggacggctga ggcgaggaga
     4921 accgacagtg cacgtcgtca tcatcgggaa cagcgccgcc gggctcagcg cgctcgacag
     4981 cctcaggcgt cgcgaccgcg cggccacggt cacggtcgtc tcgagcgagg gcgcgagacc
     5041 gtactcacgc gtgctgcttc cgtactacct ccgaggtcgt atcccgcacg cgggcctgtt
     5101 catccggccg gaggatcact acgaacggct ggacgcccgg accgcgctcg gtgaggtcgt
     5161 cgagcgggtg gacgcggagg agcacagggt cgtgctcgcc agcggccgct ccatcggcta
     5221 cgaccgcctt ctcgtggcga ccggatcgcg gcccggcgct ccccccatcg aaggcctggc
     5281 ggggcccggc gtccaggggt tctggacgct ggaggacgcg gagcggctcg accgtgacct
     5341 gcggcccggc gcccgggtca tggtcctcgg cgccgggttc gtcgcgctgc aggccgcgtg
     5401 ggcggcccac cgacggggcg cctccgtcac ggtcgtggaa ctcgagggcc gcatcctgcc
     5461 gcgggtgctc gacgaggagg cggcccgtct tcttcgcgcg cacatcgagc ggacgggcgt
     5521 gcgtatcgcg acgagcgtgt gcatctccgg cgtcgagcgc gcctgcggcg agctctgttg
     5581 caccgctgcc ggcaccgaca cctacgaggc cgacatcgtg atcgtcgcca ccggcgttct
     5641 accgaacgac ggtcttctgc cggaggcggt ctcggcgcag ggtcccggcc tcgaagtggc
     5701 gacgacaatg gagacggtcg tcgcagacgt cttcgccgcc ggcgacgtcg cccgggctcc
     5761 gatgtggggt ggcgctgtgg gcgtgctcgc cctctggccg gtcgcgatcg cccagggtcg
     5821 cgtggccggc gccaacttgg ccggcgccgg actcgagtac gagggtggct tgagcgcgaa
     5881 cgtcactgag atgttcggtg tgacggtggc ttctgcaggt cgcttcgagg aggtgccggg
     5941 cgaggaggta gtagtcttgc gcgatctgcc cggggtcgag tacctcaagc ttgtgctgac
     6001 cggaggcgtc cccgtcggcg ccgtctacct cggcggccag caggcggtcc ggctgctcgg
     6061 ccggctgggt ccgtacatcc ggcacggccg gccgctcggc gacccgcgcg ccttcttcga
     6121 agagcggcgc tccacgggga tggtcgccgt gaccccatgg tttgcccacc gcgcccccga
     6181 tcttggctcg gcagaaagga ccgtcacatg cgcatcgtag tcggcgaccc cgagtactgc
     6241 gttgcctgtc gcgattgtgc gtatgcgtgc gccttcgctc acggcggcgg gttcgaccgc
     6301 gagtcgtcgc ggatcgccgt gagcttctcc ccggaggacc gtgtctgcct gccgctcacc
     6361 tgcatgcact gtgcggaggc ctggtgcatg gaggtgtgcc ccgcggacgc gatctcgcgc
     6421 gacctcgaga cgggggcagt cgtcatccac gaggagcgtt gtgccggctg caagatgtgc
     6481 ctgctcgcgt gccccctcgg cgcggtcggt ttcgatgctg ccgccggggt cgcacgcaag
     6541 tgcgacctgt gcgacggcga tcccgcctgc gtgcgcagct gtacggtgga cgtcctccag
     6601 tactgcgagc cgggagagat ggcggactcg cggagggagt cgcttgaagc caggctccgc
     6661 gcggtgtacc gcaaaggtga aggcgggtcg tgatggaggc accgccacag accgtccaga
     6721 cgctgtgtcg catgtgcgac gaccgctgcg gcatcgacgt ccgtctcgaa gatgggcgtg
     6781 tcgtggacat caccggcaac ccggcacatc cgtggaacct tgggcgcctg tgtgtcaagg
     6841 cgagggcggc agtcgacacg gtcaaccatc ctgatcgtct gctgcaccca ctcaagcgcc
     6901 acggcgacga ttgggaggag atccccctcg agcaggcgct cgacgagatc gccgagcggc
     6961 tgcgggcgat catcgagaag gacggcgctc gcagcgtgag cgtctggaag ggtgaggcca
     7021 tcgggttcgc gcagcaggag gacctggcgc gccgcttcat ccacgcgatc ggcagtccca
     7081 actacttgtc caacgactcg atgtgctggg tgggccggtt cacggggtac aagctggtcc
     7141 tcggggcctg gccgaacgtc gacatcgaga acgcgcgctg cgtggtgatg tggggcgcga
     7201 acccgcccca ctcgcgcccg aacctgacgc agcgcatcac caaggcccgc cgccggggcg
     7261 ccacgctcgt cgtcgtcgac ccgcgcctgt ccgcggtcgc gcggcgtgcc gacctgcacg
     7321 tggcgccgct ccccggtact gacggcgccc tcgcactcgg gctcttccac gagctcgtcg
     7381 agagtggatc ctacgaccgc tcactcgtcg agcggacgac ggtcggcttc gacgacctcg
     7441 ccggctacgc ccgggagttc acccctgacg tcgtcgagcg agagaccggg atcccgccgg
     7501 ccacgctctc ggagatggcg cgtctgctcc gcgccgccga cgggcgagtg gcgctctacc
     7561 cggggaacgg cctggagcac cacgagaacg gcgtggacaa catccgcgcc atcctctcgc
     7621 tgaacgcgct gctgggctcc ttgggcaagg tcggcggctc tctcttcgcg ccgccgctcc
     7681 cgctccggga cctgacgttg tacgacgagc gaccgctcac ggagctccgg cccctcggcg
     7741 ccgaccgctt ccccgtgctc tacgaggtgc gtcgcgagtg ccacacgatg acggcgctga
     7801 acggtatcct caccggcgcg ccgtacccgc tgcgcgccat gctgctcgcg ggcgcgaacc
     7861 cggcgctcac gaatcccaac tcccacagag tcgtggaggc gctcacgaag ctcgacctgc
     7921 tcgtcgtgcg cgacctcttc atgaccgaga cggccgcgct cgccgactac gtcctcccgg
     7981 ccgcctcctt cctcgagcgg accgaggtcc acacccatca cgagatcgga gtcgtgacgc
     8041 tcacggccgg cgtcggccgg catccggagg tccagaccga gtacgagttc tggcgcgaca
     8101 tggcgcaccg tctcggcgcg gcggagtatt tcccgtggga ggacgaccgg gagctgaacc
     8161 gctggctgct cgagccgacc gcgatctcgc tcgaggagct cgaggcgcat cccgaaggcg
     8221 tgacgtacag ctcgccgccc gcagacgccg agatcgagcg gttcccgacg ccctccggca
     8281 aggtcgagtt cgtgtcgaag catctcgccg aactcggcta cgacgccctg cccaagtacc
     8341 ggcgcccggc gtacatcgag acgccggatc ccgcatatcc ctttgttctc atgaccggcg
     8401 cccggtcact cctgttcggg cacagccgct cgcacaacat ctcgcggttc gccgaggagg
     8461 ctccggcgcc ggggctggag atgcaccccg acgacgcgtc cgttctcggc ctcgccgacg
     8521 gcgacacggc gcgggtgacg acgcggatcg gatcggtcga ggcgccggtg cgcgtggtcg
     8581 ccaccaacga gttgccccgg cactgtctgc agctgacgca cggctgggca ggcgtgaacg
     8641 ccaacgccct cactcacgac gaccgcttcg accgcatcag cggattcccc ctgatgaagt
     8701 cggtcgaggc gcgcgtcgag ccggtgggcc ggaccggatg acgagcgtgc ggcgtcgccg
     8761 gccattgccg tcccagccga tggacggccg actggggctc gttgacatcc cgtcgtgggc
     8821 cgatactgtc ctcctgacag cgagcgagtg aggaaggatc tttggtcgag ccgaacgtca
     8881 aggacgagct cttcgacacg gcgatcatgg acgaggaggc cgtcaagggc ttctccgacc
     8941 cggcccagca ggagcggccg aggacgtgga ggctctattg gcgccgcctc ctcaagcgtc
     9001 cggcggcgct cgtcggcacc gtcatcttct gcttcttcct cttcttggcc gtcttcggcc
     9061 cctggatagc cccgtatgac tacgagaagc agagcgccga gctgcgcctc gcgacgccca
     9121 cgctgagcca tcccttcggc gccgaccagt tcgggcgcga catcctcagc cgcatcatcg
     9181 tcggcacgcg caacatcttc ctgctcggcg ggttcgggac cgcgatcgcc gtggtcatag
     9241 gcaccgccat cggcctgttc tcgggctacg tcggcgggag catcgacgag gtggtgatgc
     9301 ggttactcga cgtgctcttg tcgttcccct cgctgctgct cgcgctcgtc ctgctgagca
     9361 ccgtagggcc ctccaacatc aacatcgtgt tcgtggtcgc gatcctctac atccccatgg
     9421 tcgcgcgtgt ggtgcgcagc atggtgctcg acctcaagaa caaggaattc gtcgaggccg
     9481 cgcgggtgcg tggcgagacg cgcagctacg tgctcttccg cgagatcctc cccaactcgc
     9541 tgccgccgct gctggtggag gcgtcgatgc gcttcagcta ctcgatcttc ctcgtcgcct
     9601 cgctggggtt cctcggcctc ggcgtgcagc cgccgtcacc cgactggggg ctgcagatca
     9661 accaagcgcg cgccttcttc gccatcgcgc cgtgggtact gctcttcccc gcctgcacca
     9721 tcgccgtgct cgtgatcgcc accaacctga tgtcggacgg gctgcgacag gtcatgctcc
     9781 ccggaggcgg gaggaactga tggctgcccc cgcctcccgc cccgtcttcg agatcgaggg
     9841 cctctgcgtc gactacacca ccgagctcgg cgtcctgcac gcggtacgcg acgtctcgct
     9901 cgccgtccac gagcacgaga gcttcgggct cgtgggcgag tccggctccg gcaagtccac
     9961 gctcgccatg ggcgccatcc gctatctggc gcagaacggc cgcgtcacgg acggcagcgt
    10021 ccgcctcaac ggcgtcgagc tgctcgacct gccggacagg gcgctccggg ccctgtgggg
    10081 cgcgaagatc ggcgtcgtct atcagagccc gctgagcgcg ctcaacccgt cgatcgtgat
    10141 cggcaagcag ctcgccgagg tggcgcgcca gcacctcggg atggacgcgg ccgcagcccg
    10201 cgaccgcgtc cacgagatgc tcaccaaggt cgccatgccc gaccccgagg cggtgacgaa
    10261 gcgctacccg caccagctct ccggcggcat gctgcagcgc tgcgtcatcg ccatggcgct
    10321 catgaccgac ccatcgctgc tcatcatgga cgagccgacc acggccctcg acgtcaccac
    10381 ccaggccgtc gtcctcgacc tcgtcgccga gctcaagcgt gagttcgact cggcgatcct
    10441 gtacatcacc cacgacctcg gcgtgatcac caagatctgc gaccgggtgg gcgtcatgta
    10501 cgccggcgag ttcatggagc aggccgagct gaaggagctc ttcaagcggc cgctgcaccc
    10561 gtacacgctc gacctgctcg gctgcgtgcc gcacttcgac cccacgccgc agaagcgctc
    10621 cctggtgacg atccccgggt cgatcccccg cgtcgaccag ctgcccccgg gttgcatctt
    10681 cgcgccccgc tgcggcttcg tcgaggaggc ctgcacgcaa gcgcgtccac cgctcgtggc
    10741 cgtccagggc gcgcacctct ccgcgtgccg gcgctggcag gccgtgccgc cgccggccga
    10801 gtacctgcgc gccgcgaccg aggccgtgcg cgaggtccag actgagtgcg tcgctcgcga
    10861 ggtcctcgtc gcggccgacg acacccaggt gcacttcaag gcgcccaagg gcacggtgcg
    10921 cgccgtcgac ggggtggacc tcgaggtgtg cgtcggtcag acgctcggag tcgtcggcga
    10981 gagcggcagc ggcaagacga ccgtcgcgcg cgccatcatc ggcctgacac cccggacgag
    11041 cggggacatc aggctgcggg gtgactccct ggcgctgtcg acggcgaagc gaccgcgctc
    11101 cacactgcgc gagatccaga tggtgttcca gaacccggac gcctcgctca acccgacgcg
    11161 cagcgtcggc gatgcgatca tgcgcccgct cgccctgctg ggcggtctgg accgccgcca
    11221 ggccaaggac aaggcgctcg aactgctcgc atcggtgagt ctgccggcca gctacttcga
    11281 ccgcctgccg cacgagctct ccggcggcga gaagcagcgc gtggcgatag cgcgcgcctt
    11341 cgccgccgag cccggcatca tcctctgcga cgagcccatc agttcgctcg acgtctccgt
    11401 gcagggttcg ctcatgaacc tgctcatgaa gctgcaggtc gagaagcaga cgtcgtacct
    11461 gttcatcagc cacgacctct ctgcggtgca gcacctttcc gacgtcatcg ccgtggtgta
    11521 cctcggccac gtcatggagg tgggtgacgc gaccacggtg cttacgccgc cgttccaccc
    11581 gtacacggag gcgctgctct cagccgtgcc gctcgccgat ccggacgtca aacagaagcc
    11641 gatccgcctg agcgggtcgg tgccgagcgc gatgaacgtc ccgtcgggct gccgcttcca
    11701 cccgcggtgc ccgcgctgcc tcggcgacgt ctgccgcgag caggagcctc cgtggcgcgt
    11761 gggcgaaggc gaccaccgca tctactgtca catcccgctg gacgagctgg cgcggctgca
    11821 ggccgagacg ctcgtcctcg acgccgaaga gagcgcctga tggccaggtt catcctcagg
    11881 cgcctcgggt tcatggtgat gaccgtggtg ctcgcctcga tcatcatctt ctgggcgacg
    11941 accgtcctgc ccggtgacgt ggccagccag atcctcgggc gcttcgccac ggaggaggcc
    12001 aagagcaatc tgcgcaccga gctcggtctg gacaggcccg tcgtcgtcca gtacggctcg
    12061 tggctgggca actacgtgca gggcgactgg ggcgactcgc cgagcatgaa cacgctcgtc
    12121 cgccccatcg tcttcgagcg cctgggcaat tcgctcatgc tcgccgtcgt cgccttcgtc
    12181 atgtacgtgc cgttagggat cctgctcggg ctcttggcgg cgttgcgcaa gaacacctgg
    12241 gtggaccaga cgatctccat cggctcgctc gcgttcatcg gtctgcccga gttcgtcagc
    12301 ggcctcatcc tcatcgcgat cttcgccatt cagctccagt ggttcccgtc gcagtcggcg
    12361 atcccgccgg acacaggctt catcgaggcg ctgccgtacc tcatcctgcc ggcgatcacg
    12421 gtggcgctga ccagcctggc ctacgtcgtg cgcatgacac ggtcgagcac ggtcgacgtg
    12481 ctgcagatgg actacgtgcg gaccgccaac ctcaagggcc tgccgccgag gaaggtcctc
    12541 ttcacccacg tgctgcgcaa ctcgctgctg ccgacggtga cggtggtggc catcggcatc
    12601 ggctggctca tcggcggcct gatcgtgacc gagtcggtct acgggtaccc ggggatcggc
    12661 cgcctgctgc tcttcgcgat ccagcgccgc gacctcgttc tcatccaatc gacggcgctc
    12721 ctcatcgtgc tcatctacgt ggtcgcgaac ctggtcgccg acatcctcta cgcggtcctc
    12781 aacccgcgca tccggtacgg ctgatcggcg gacgccgcgg ctgcgctcca gaggcgggga
    12841 ggcgggtcac gccggaaaac gctcctgtat tcgagactgc gagcacgcgc tcttgcccgt
    12901 gaagtagccc ttcgtcgata gtccctcgtg cggactccgg gccgccgacc cggtccgaac
    12961 cacggtcaca ggagtgtgca catggggact tcactgcgcc tgacggtgtg cggcgccggc
    13021 ggcgccgggc tggccatcgc cgcggacaac gcgctcaagg gcatcgacgt gactctgttc
    13081 gagctcccgt cgctggcgga gaagctcgaa ggcccgatag cccgcggcgg cgttgaggtg
    13141 acggccggct ccgagacgac ggccggcaag accggctttg cgccgctgac ggcgacgagc
    13201 gacgccgcca aagcggtggc cggcgcggac gtggtcatga tcaccgtccc cgcgatgtac
    13261 cacgacgtct tcatggacgc cgtggctcct cacttgagcg acggtcagat cgtgctgttc
    13321 aacaccggct actggggctc gctgcggcag gccgtgcgct gggcgggaag actgccggac
    13381 gtgtccctcg ccgagtccaa catcatgccg tacatctgcc agcccaaggg cgacgccatc
    13441 cacatcgggc gcttcaagcg gcacttctgc gtcgccgcct tccccggcga gcggagcggc
    13501 tccgtgtacg acgtcgtgcg ccggatctac gagcagtacg atccggcggg cacggtgatc
    13561 gacaccaaca tcgcggccgc cggaaacccc ccgatccacg cgacgctcac catccccgtg
    13621 gccggctatt acttcgaccg ctacatgggc ggcaagttct atcaggacac cacagtgccc
    13681 ggcgcccggc tcgtggaggc attcgacgcg gagcgccagc tccttgcgac gcggctcggc
    13741 tcgcagctgt tcgaggacca actgagcttc gaccgccgct cgtacctcta tgagggcgcc
    13801 ggcctcgtgg aggcgctgcg ctcctcccca cacgccgact ggtacgcgac cgccgactac
    13861 ctggaacagg tgtgcagcga ggacatcatc tatgcgttcg tccccatggt gcggatcggc
    13921 gaggcgctgg gcatagacct gcctgtgacc cgttccatga tcgaggtgat gggcgtgatg
    13981 ctgcagcgtg actactggac cgagggcctg gacctcgccg ctctcggact cgacgggctc
    14041 gatcttgacg gcgtgcgacg gttcgtcatg accggggagc gctgagagca ggcgtggccg
    14101 tcgtcctgac gggcaggagc ctgacgctcg acgaggtcgt ctccgcggcg cgcggcggcg
    14161 agcaggtcgt gctggcggag gaggcgctga cggcgatggc tgccgcgagc gccctcgccg
    14221 cgggtatcgc cgagcgaggc gcgcccgtct acgggctcac caccgggctc ggcgtgcaga
    14281 agcggacgcg gcagcagcgc gacgactccg gcttcagctg gcggcagatc gccgagagcc
    14341 gcgccggggt cgggccgctt gcgcccgccg acgtggtccg cgcggcgatg ctcgtgttcg
    14401 ccaaccagat ggccggagcc acgacgtgca tccggccggt cctcgccgag cgcctcgtcc
    14461 gggcgctgaa cgacggcgag cgcccggagg tccgcgcgcg cggctccatc ggcgcctcgg
    14521 atctggcgca gatgtccgac ctcgccgccg gggtcttcgc cggcatggac ctcgtccagg
    14581 gcgagggcct tgccctgatc aactcgagcg ccttcgggac ggggtcggcg gcgctggcgc
    14641 tggccgacgc cgcccgcctg ctggaggccg ccgacgtggc ggcggcgctc agcctcgagg
    14701 gcttcgccgc caacctctcg gtgctgcacc cggccatcga gcgcgcgcgg cccgaccccg
    14761 tgctcgcgca cacactggcc cggttgcgcg agctgctcca cggcagctac ctgtgggacg
    14821 agggcgcggc gcggaacctt caggacccgc tcaacttccg gagcgcggcg ccggtccagg
    14881 cggccgcgcg ccgcgccctt gagtacgcgg ggtccgtcgt ggcgatcgag ctcaatgcgg
    14941 cgcagggcaa ccccctcgtc tcggtggagg aagggacgat ccgctcggcg tgcctgtatg
    15001 aggtcgtggg cgtctccgcc gcgctcgact tcgtcaggat cgcgttcgcc tccatgctgc
    15061 tggcggcgtc cgagcgcagc gtcaagctga tggacacgcc gtggtccggg ctgccggtcg
    15121 ggctccagga gcccggcagc ccggacctcg gcctcagcat cctcgccatc acgtcggaag
    15181 cgctgggagc agaggtcgcg acg
//
LOCUS       DASUMX010000304         3715 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_693516, whole genome shotgun sequence.
ACCESSION   DASUMX010000304 DASUMX010000000
VERSION     DASUMX010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3715
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693516"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VFZ86_14315"
     CDS             <1..366
                     /locus_tag="VFZ86_14315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297865.1"
                     /note="protein from Staphylococcus aureus has
                     phosphodiesterase activity against 2'-3'-cAMP and
                     2'-3'-cGMP; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEX5643492.1"
                     /translation="CHAIQAHHQDVEPQTVEAVLVQAADAISAARPGARRESLENYIK
                     RLESLEHIAEAHKGVEKCFAMQAGREVRVMVKPAEIDDDATALLAREIAREIEDQLDY
                     PGQIRVTVIRESRAVDIAK"
     gene            461..1786
                     /gene="miaB"
                     /locus_tag="VFZ86_14320"
     CDS             461..1786
                     /gene="miaB"
                     /locus_tag="VFZ86_14320"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEX5643493.1"
                     /translation="MTGRYYLRSFGCQMNEHDAERIRALLEAQGLERADEPDGADVLV
                     YNTCTVRKSADDRLAGHLGTAARLKREDPSRVVLVTGCLPQAEQAGFFARFPFVDGAL
                     GPQNLHRLPELLRAAVEPAEGAPAGYFEDGPHFSGSLGARRERPFQAWLQVMSGCTNF
                     CSYCIVPHVRGPERSRSIEDLVAEAEALVADGVREVTLLGQNVNAYGRDLWRRGEAAA
                     PTFAALLARLDAVPGLERIRFMTSHPRDLGDDLVAAVASLPSVCEHVHLPAQAGSDRV
                     LAAMRRGYTVARYLERVRALRAAVPDVSITTDLIAGFPGETEDDFAQTLSLVREAAFD
                     AAFTFVFSPRRGTAAAELPDQVPPDVRRERVERLIALTQQQALASRQAWVGRRAEVLV
                     EGPSRDGVRHRGRTRQNVTVNLSGAVEAGAIVLVEVTGATSTTLRGTTV"
     gene            1810..2604
                     /locus_tag="VFZ86_14325"
     CDS             1810..2604
                     /locus_tag="VFZ86_14325"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol dioxygenase subunit B-like
                     domain-containing protein"
                     /protein_id="HEX5643494.1"
                     /translation="MQGCICPHPPLLIPEVGGTARNKIATTVMAMERLAASVGDPDTI
                     VVISPHADDFGAAHAVKTAPRLRGDFGRFRCPEASFSYDNDVQFAELLLALAGDNRKI
                     RLVPDDGDVLDWGVLVPLSFMRPNRIVSLSVVGPYAEHRTLGQLVRRCAEELKRDTLF
                     LASGDLSHALIHGAPAPYDPRGKLFDDEIVSLLGVGDFPALTRIEPVLLDGAAECGLR
                     SFVALGGFLGDDAMVDPEILSYEGPYGVGYLVARFGPRPVSADAEG"
     gene            2601..3173
                     /gene="amrA"
                     /locus_tag="VFZ86_14330"
     CDS             2601..3173
                     /gene="amrA"
                     /locus_tag="VFZ86_14330"
                     /inference="COORDINATES: protein motif:HMM:NF013988.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HEX5643495.1"
                     /translation="MSDPGEAAPGPADYALSCVRSYAAGGRAPEPPAGAFYERRAACF
                     VTLKKRGDLRGCIGTLEPAEASLGWEIARNAQSSALRDPRFPPVTPDECDALTCSVDV
                     LSPSEACTVDDLDPRCWGVIVEAGPRRGVLLPALEGIDTVDAQVGIALQKAGIAPGQA
                     YDVRRFLVTRYRQGDPPVVSSAQAGDPTDD"
     gene            3166..>3715
                     /locus_tag="VFZ86_14335"
     CDS             3166..>3715
                     /locus_tag="VFZ86_14335"
                     /inference="COORDINATES: protein motif:HMM:NF013845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl transferase family protein"
                     /protein_id="HEX5643496.1"
                     /translation="MTDAPPVVALFGATALGKSDVALELAALLDADIVVADSMQIYAG
                     LPVVTNQPGAEARRRAAYHLVGWASPQDEFSVAEYAAMAHETVDRLLEAGRPVIVEGG
                     SGLYLRAALGDLAFGGAPDAARRRALEERWERAPAALVAELRRRAPDVAARVDLRNAR
                     RVIRALESLDREGAGQVQTDELW"
BASE COUNT          495 a         1417 c         1320 g          483 t
ORIGIN      
        1 tgccacgcga tccaggcgca ccaccaggac gtggaaccgc agaccgtgga ggccgtgctc
       61 gtgcaggcgg ccgacgccat cagcgccgcc cgtccgggcg cccgccgcga gtcgctcgag
      121 aactacatca agcgcctgga gtcgctggag cacatcgccg aggcccacaa gggggtcgag
      181 aagtgcttcg cgatgcaggc agggcgcgag gtgcgcgtca tggtcaagcc ggccgagatc
      241 gacgacgacg ccacggcgct cctggcgcgc gagatcgcgc gcgagatcga ggaccagctg
      301 gactatcccg gccagatccg cgtgaccgtc atccgagaga gccgcgccgt cgacatcgcc
      361 aaatagccgg tgtgcctgcg gcgctccgcc agttgagccc gctacccagc tcaatagcgg
      421 gcgccgtgaa ggccgccccc gcgagcgcga cgataccgcc atgaccggcc gctactacct
      481 gcgctcgttc ggctgccaga tgaacgagca cgacgccgag cgcatccgcg cgctgctcga
      541 ggcccagggc ctggagcgcg ccgacgagcc cgacggcgcc gacgtgctcg tctacaacac
      601 ctgcacggtg cgcaagagcg cggacgaccg cctcgccggc cacctcggca cggcggcgag
      661 gctcaagcgc gaggacccct cgcgcgtggt cctcgtgacc ggctgcctgc cgcaggccga
      721 gcaggcgggc ttcttcgcgc gcttcccgtt cgtcgacggc gccctcggcc cgcagaacct
      781 gcaccggctg ccggagctgc tgcgggccgc agtggagccg gcggaagggg cccccgccgg
      841 ctacttcgag gacggtccgc acttcagcgg cagcctcgga gcccggcgcg agcgcccgtt
      901 ccaggcgtgg ctgcaggtca tgagcggctg caccaacttc tgctcctact gcatcgtccc
      961 gcacgtccgt ggccccgagc gcagccggtc gatagaggac ctggtcgcgg aggccgaggc
     1021 gctggtcgcc gacggcgtgc gcgaggtgac cctgctcggc cagaacgtca acgcctacgg
     1081 gcgcgacctc tggcgccggg gggaggcggc cgcgcccacg ttcgccgcgc tcctcgcgcg
     1141 cctcgacgcc gtgcccgggc tcgagcgcat ccgcttcatg acgtcgcacc ccagggacct
     1201 cggagacgac ctcgtcgccg cggtcgcgag cctgcccagc gtgtgcgagc acgtgcacct
     1261 gcctgcccag gccggctcgg accgcgtgct cgccgccatg cgccgcgggt acaccgtggc
     1321 gcgatatctc gagcgcgtcc gcgcgctgcg tgccgccgtg ccggacgtca gcatcaccac
     1381 cgacctcatc gccggcttcc ccggcgagac ggaggacgac ttcgcgcaga cgctgtcgct
     1441 ggtgcgggag gcggccttcg acgcggcctt cacgttcgtg ttctcgccgc gtcgcggcac
     1501 ggccgcggcc gagctgccgg accaggtccc gccggacgtc cgccgggagc gtgtcgagcg
     1561 cctgatcgcg ctgacgcagc agcaggcgct cgcctctcgc caggcctggg tcggacgccg
     1621 cgccgaggtg ctcgtggagg gccccagcag ggacggcgta cgccaccgcg gccgcacccg
     1681 ccagaacgtc acggtgaacc tctccggcgc cgtggaggcc ggtgctatag tgctggtaga
     1741 agtgaccgga gccacctcca cgacgctacg cgggacgacg gtctgagccg gcgaccagga
     1801 ggcatcgtca tgcaaggctg tatctgccct catcctccac tgctcatccc cgaggtgggg
     1861 gggaccgcgc gcaacaagat cgccaccacc gtgatggcga tggagaggct cgccgcgtcc
     1921 gtcggcgacc ccgacaccat cgtcgtcatc tccccgcacg ccgacgactt cggcgccgcg
     1981 cacgccgtca agacggcgcc gcgcctgcgc ggcgacttcg gccgcttccg ctgcccggag
     2041 gcctccttca gctacgacaa cgacgtgcag ttcgccgagc tgctgctcgc cctcgccggc
     2101 gacaaccgca agatccgcct cgtgcccgac gacggcgacg tcctggactg gggcgtcctg
     2161 gtgccgctca gcttcatgcg gcccaaccgc atcgtgagcc tctccgtggt gggaccctac
     2221 gccgagcacc gcacgctggg gcagctggtg cgccgctgcg ccgaggagct caagcgggac
     2281 accctgttcc tcgcctcggg cgacctctcg cacgcgctca tccacggcgc tccggcgccg
     2341 tacgaccccc gcggcaagct cttcgacgac gagatcgtca gcctcctcgg ggtcggcgac
     2401 ttcccggcgc tcacgcgcat cgagcccgtc ctgctggacg gcgccgccga gtgcggcctg
     2461 cgcagcttcg tggcgctggg cggcttcctc ggcgacgacg ccatggtcga ccccgagatc
     2521 ctgagctacg aaggccccta cggggtcggc tacctcgtcg cgcggttcgg gccccgcccc
     2581 gtctccgcgg acgccgaggg atgagcgacc cgggcgaggc ggcgccgggt ccggccgact
     2641 acgcgctgtc ctgcgtccgc tcctacgcgg ccggagggcg ggcgcccgaa ccgcctgccg
     2701 gcgcgttcta cgagcgtcgc gcagcctgct tcgtgaccct gaagaagcgc ggcgatctgc
     2761 gcgggtgcat cggcacgctg gagccggccg aggcgtcgct cgggtgggag atcgcgcgca
     2821 acgcgcagtc gtccgccctg cgcgacccgc gcttcccgcc cgtcaccccc gacgagtgcg
     2881 atgcgctcac ctgctcggtg gacgtgctca gcccgagcga ggcgtgcacc gtcgacgacc
     2941 tcgatccccg gtgctggggc gtgatcgtgg aggccgggcc tcggcggggc gtgctgctgc
     3001 cggcgctcga ggggatcgac accgtcgacg cgcaggtcgg catcgccctg cagaaggccg
     3061 gcatcgcgcc cggccaggcc tacgacgtac gccgcttcct cgtcacgcgg taccgccagg
     3121 gcgatccgcc ggtcgtctcc agcgcacagg ccggcgaccc cacggatgac tgacgcgcca
     3181 ccggtggtcg cgctgttcgg cgccaccgcg ctcggcaaga gcgacgtggc gctcgagctc
     3241 gccgcactcc tggacgccga catcgtggtg gccgactcca tgcagatcta cgcgggactg
     3301 cccgtcgtca ccaaccagcc cggcgcggag gcgcgccggc gcgccgccta ccacctcgtc
     3361 ggctgggcct cgccgcagga cgagttcagc gtcgccgagt acgccgcgat ggcgcacgag
     3421 acggtggatc ggctgctcga ggccggcagg ccggtgatcg tggagggcgg ctcgggcctg
     3481 tacctgcgcg ccgcgctcgg cgacctcgcc ttcggcggcg cgccggacgc ggcgcgcagg
     3541 cgcgcgctgg aggagcgctg ggagcgcgcg ccggcggccc tcgtcgcgga gctccgccgg
     3601 cgcgcgccgg acgtggcggc gcgggtggac ctgcgcaatg cgcgccgcgt gatccgcgcg
     3661 ctcgagtccc tcgaccgcga gggcgccggc caggtccaga cggacgagct gtggc
//
LOCUS       DASUMX010000305        15757 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_693858, whole genome shotgun sequence.
ACCESSION   DASUMX010000305 DASUMX010000000
VERSION     DASUMX010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 15757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15757
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693858"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(248..988)
                     /locus_tag="VFZ86_14340"
     CDS             complement(248..988)
                     /locus_tag="VFZ86_14340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643497.1"
                     /translation="MGSYRSDDEALIGLARQLTDAYAEARTREDQDYACDSIAEYLSE
                     FRDTAPSEALLAVLDLPLTVETYPLVDEAQVALAARGPGVVELLLEAVLGDVYDPDGP
                     APERAAETLDAMDPRAAATGLCDVLCGRGDDQIKGAALDALVALGGAAEPDLTVTRDD
                     PVGGEWARAALEQLAFEREHPGAEVIASDEAAAEDEGPTAAEDGESDAPEQAPPQAES
                     APDQGVVDDAYDDFLRRYGQEQGEEPLP"
     gene            complement(1041..1469)
                     /gene="msrB"
                     /locus_tag="VFZ86_14345"
                     /pseudo
     CDS             complement(1041..1469)
                     /gene="msrB"
                     /locus_tag="VFZ86_14345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013515395.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
     gene            complement(1606..1992)
                     /locus_tag="VFZ86_14350"
     CDS             complement(1606..1992)
                     /locus_tag="VFZ86_14350"
                     /inference="COORDINATES: protein motif:HMM:NF014620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HEX5643498.1"
                     /translation="MNQHDQHILRLAIPSDLPGGLDAPRSGHFRRSPCFTIVDLSDDV
                     VVDVRVVTNPKRHGDHGLTPILTLGENLVDIVIVAGIGRKALLHCIQAGIRVLAGDDR
                     SDVRSVVQAFIDAELVPVGGDATAAH"
     gene            2126..3106
                     /locus_tag="VFZ86_14355"
     CDS             2126..3106
                     /locus_tag="VFZ86_14355"
                     /inference="COORDINATES: protein motif:HMM:NF021617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HEX5643499.1"
                     /translation="MPLGGYCNVCQRWVWLTPYGECQNGHPALAVKDVQQLKPQSEPD
                     VLVGEVVDESKPRARFRWWWRHSVWILWMFTLGFMNWLGFVYVGVRARHTPWTLYGFA
                     YLVPLLLTITAVATALPVWPFFLLQLLVSVLTVAHALSIRPYYRAIMFGDASPKSLPA
                     APQPPVLAAGSRPELPRNIDERAADVLQPARRQVDKILGTASSIGKPAVRDEVVGLCT
                     TADQIIAELASQPRKLDAARGFLTYYLDAAQRIVEGYVNLARRGGTAPEISQTLARAE
                     ASLKVVQEAFDRQLANVLEDRAMDLDSEIELLERTVRSETMYTQTSGELR"
     gene            3156..4400
                     /locus_tag="VFZ86_14360"
     CDS             3156..4400
                     /locus_tag="VFZ86_14360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010662062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HEX5643500.1"
                     /translation="MSDDIQKQDEPTSWGTGSDVEDQAAPDVAGVPAVRDAAGADPAA
                     VAGDQPAAGPLSPADLQRVAEISRQVDVGDTQAVLSYGLPAQSRIANFADSLLGDVRN
                     KDAGTGGEALQDLLKKVREIDVDALSAGTGKAKIPILGRFANTFERFSARYQKVATSI
                     EKIIDALERSRMGLLKDMTVLDKMYELNLDYLKQLDVYIAAGEQALQELHAVKIPALE
                     AEVAASDDVMAAQRLADLQQAATRFERRIHDLKLTRMIAIQTAPQIRLIQSNDQNLVE
                     KIQSSILTTIPLWKNQIVIAISLYRQQKAMELQKQVTDTTNELLAKNAELLRDGSAKV
                     AREVERGVVDVETLRKVNADLIATLEETIRIQEEGRAKRVEAEGEIGRLQVELRQKLI
                     ELRSQAPQIGTGPGEPRRLTDY"
     gene            complement(4408..4869)
                     /locus_tag="VFZ86_14365"
     CDS             complement(4408..4869)
                     /locus_tag="VFZ86_14365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018397833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HEX5643501.1"
                     /translation="MECVWDYPRPPRVEASHKLVRVEFAGEVIAETTRAYRVLETSHP
                     PVYYIPPEDVRSDFLRPSRRRTWCEFKGEASYYDLVAGGREVRDVAWYYPEPTARYTA
                     LRDHVAFYPGRVDAAYVDGEQVAAQAGDFYGGWITADIEGPFKGGPGTAGW"
     gene            complement(4878..5324)
                     /locus_tag="VFZ86_14370"
     CDS             complement(4878..5324)
                     /locus_tag="VFZ86_14370"
                     /inference="COORDINATES: protein motif:HMM:NF024988.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX5643502.1"
                     /translation="MSEEQEYSAKDVALAVRRLDLMMSQMHLEMSERLGLSAAELLAL
                     AHLSVAGSLGPTELTRRLHMTTGAMTAMLDRLAEREFIVREPHPSDRRRIVVRLTGEG
                     RDRIFTQVHGMADEVVAISDRLDPGARRGVIRYIEELCGAIGAPEA"
     gene            5461..5874
                     /locus_tag="VFZ86_14375"
     CDS             5461..5874
                     /locus_tag="VFZ86_14375"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HEX5643503.1"
                     /translation="MTDSDRAAYTVAHIDDMPFEEGSSPGTEWKPVRRIFGIGSFGTA
                     LARATAAGDTLTHDHDEVAARHEELFLIVSGHATYRVDGEEIDAPAGTFLYVPDPATV
                     RGAVAVEAGTTMFVVGAEPGASFTPSDWDTVPLPE"
     gene            complement(5921..6439)
                     /locus_tag="VFZ86_14380"
     CDS             complement(5921..6439)
                     /locus_tag="VFZ86_14380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008554350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5643504.1"
                     /translation="MATRTEPLKLDPPTLDAGTGIGHVHLKVSDLDRALAFYQGVLGF
                     ELTGRLGDQAAFVSAGGYHHHIGLNTWESRGGSPPPRSATGLYHVAIRYPTRAALARA
                     LRALIERRYPIEGASDHAVSEAIYLHDPDGNGIELYWDRPRDGWPVNPDGSYAMVVEP
                     LDLDGLLAEADD"
     gene            complement(6488..6958)
                     /locus_tag="VFZ86_14385"
     CDS             complement(6488..6958)
                     /locus_tag="VFZ86_14385"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX5643505.1"
                     /translation="MKQTPTDAGRERAAMELWTELTSFTGAVRARLEHRLDAELGVLP
                     EEAELLARLDEAPEQRLRMADVSRSLRLSKSGVTRLVDRLADRGLVVRAACPKDRRVV
                     YAGLTDEGRRAAASAAPAVAAGVAEQLAGGLSAEQLDSLTASLRALRGPGEAGA"
     gene            7068..7709
                     /locus_tag="VFZ86_14390"
     CDS             7068..7709
                     /locus_tag="VFZ86_14390"
                     /inference="COORDINATES: protein motif:HMM:NF019603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HEX5643506.1"
                     /translation="MKIAIIGAGNVGTALAGAATRAGNAVTVSSTSGESARAAADQTG
                     ARAAASNRTALEDADLVILAVPYAAVDDVLASVGDAISGKVVVDATNPIKPDYSGLAT
                     GERSGAEEIQAKAPGARVVKAFNTAFAARQADPRVAGGLRVDGYVAGDDEAAKTAVLG
                     LAEAIGFNPVDAGGLTMSRHLEGMAWLNISLQMQHGWAWQAGWKLVGPDGDAA"
     gene            complement(7805..9478)
                     /locus_tag="VFZ86_14395"
     CDS             complement(7805..9478)
                     /locus_tag="VFZ86_14395"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5643507.1"
                     /translation="MSEHLSPRPQDAVADIDPQSDAATTDRATHVPRRLGLALVVIAA
                     AQLMIVLDATIVNVALPHIQQALGFSGTGLEWIITAYSLAFGSLLLLGGRLGDIYGRR
                     RIFMTGVLLFALASLVGGFATTGWWLLASRAVQGAGAALAAPTALALIATTFPMGAPR
                     NRALGVWAGMAGAGGAIGLLLGGILTSYVSWRWVFFVNAPIGLVVAALAPMALAGGGR
                     LRRRLDVPGVVTSTAGLALLVYGLTHAAAGPDGASRWGDRVTLASLAGAVALLVGFVF
                     IERYVREPELDLNLLRSRRRSGAYIMMLLLGTAMFAVFFFLTIYVQTVWGYSPVRAGL
                     AWVPFPIALIAINVFVARVLVAKVGVRPLLMAGPLFAGAGFMLLSRLSPTGSYWTDLL
                     APMLVLALGMGLMFVPLTLMVVSHVRHDEAGAASSLLNIGQQVGGSIGLAAIGTIAWT
                     SVSNTVRDSMAAAAASGAAQGAGGSVAGAGEGAAGVPQAILFHGLTVGFSTGLVIAGV
                     VALSGFVVAVVTTWTPGRFRLRSARHDREPSCDEVLGTCETPALSAADA"
     gene            complement(9599..11290)
                     /locus_tag="VFZ86_14400"
     CDS             complement(9599..11290)
                     /locus_tag="VFZ86_14400"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5643508.1"
                     /translation="MSLQPVILVVDDDMTSLTITEQELRKRYGRDYRVLARQSAAAAL
                     DELRRLKIEGQPVALILADQVMPEMSGPDFLGEARVIDPVAKRVMLVSWGDRSAPQAI
                     LTGCAVGQIEFFITKPWHTATDERFHSEVAGFLYEWARLQRPVYEAVRIVGERWAPRS
                     HELRDLLGRNGVAYGFYDADSGEGREILRDAGLDAPPKLPVAVVFGGRVLVDPSNREV
                     SESLGVRSRPSADECDLLVVGAGPAGLAAAVYGSSEGLSTVVLEREALGGQAGQSTMI
                     HNYLGFPAGISGDELTFRAYEQAWQFGAEFLFSNEATSLRPDGDAHMIELADGASLRA
                     GAVILATGVTYRRLAVPRLDPFRGSGVFYGSVSSEARSLVNMDVYIVGGGNSAGQAAI
                     HLAKYARHVTLVMRGDGLSDTMSHYLVSFLSNVDRISLRPRTLIVDGDGDTRLRRLTL
                     EQIDSGEREVVPADALFVMIGADPHTEWLPPEIARDAHGFVLTGPDILGAGLPEPWPL
                     ERLPLGLETSLPGVFAAGDVRADSVKRVASAVGEGAVAVRLVQQHLAARQLNRVA"
     gene            complement(11380..12900)
                     /locus_tag="VFZ86_14405"
     CDS             complement(11380..12900)
                     /locus_tag="VFZ86_14405"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HEX5643509.1"
                     /translation="MTPRYFSQSISGLEEAHPAETLAVATGGSFELKASPVRKRVGEA
                     DVRLLAYNGSVPGPTIRVRQGSEVVCRFVNGLDLDTTVHWHGLRHDYLYDGVPRGGRH
                     GGMQDPVRPGRDFTYRLRFPDPGVYWYHPHMREDYTQEMGLYGNIIVEPQDPDYWPAA
                     HRDLPLIVDDILIQDGAVAPFSVQGSSHTAMGRFGDVMLVNGETSPRFVARRGEVVRL
                     YLTNTANTRVFRLCIPGARLKLVGSDLGRIEEERMVERLILAPSERWVVDAFFPDEGE
                     YVLEHVSEGRTYRLAAFVVEAEEALPDLSGEFGRLRRNPEFAEERARWTEELEREPDR
                     VLVLQGDMPGMAMDHGRMARAGTEHSGSTPAAHSLIEWEDDMPEHNASSSPESMVWRI
                     HDEALGADNEGIHWVFAQGDRVKVRIINDPDSDHPMHHPVHFHGQRFYELRWDGGPAP
                     SIMWKDTVLVPTGKTVDILLECSNPGSWMVHCHIAEHLEGGMMFTFEVRASLAAAS"
     gene            complement(13001..13573)
                     /locus_tag="VFZ86_14410"
     CDS             complement(13001..13573)
                     /locus_tag="VFZ86_14410"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX5643510.1"
                     /translation="MPDSPTRQLADEHEYVLLVVGAMEAEASRIERSGEVDVVRVAQM
                     VDFTRNFTDGDHHTKEESLLFPLLEERSAAAGGTISVLLSEHEAARECIRAIDRALKD
                     VSVPDGERAAAARAVIVENLRLYAFLLPLHIGKEDTVLFALTDELLSVQEQEILAEDF
                     ERLASSPGAAAQTARYQRMAHDLAEAPAKG"
     gene            complement(13650..14990)
                     /locus_tag="VFZ86_14415"
     CDS             complement(13650..14990)
                     /locus_tag="VFZ86_14415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX5643511.1"
                     /translation="MEDLLHHTAARAIRYLRDLPERRVAPSPDAITGLERFDEPLPEH
                     PDDAERVIALLDEAGSPATMASAGPRFFGFVIGGSHPVAVAASWLAAAWDQNAGASTA
                     SPTAAALEQVAQRWLVELFGLPSTTVAGFVTGGTMANMTALAAARHAVLARAGWDVDA
                     KGLGGAPPVTVIAGDEVHPSLLKGVGLLGLGREQVRRVEVDGQGRMRADRLPPISGPT
                     IVCTQAGNVNTGAFDPVEEIAARAHEAGAWVHVDGAFGLWAAVAPARAHLVRGVGAAD
                     SWATDAHKWLNTPYDSGLAFVRDPEPLRAAMGLTAAYLPDSMMREPAAHAPELSRRAR
                     GVEVWAVLRALGRSGLADLVERDCRHAASFARGLADAGYEILNDVVLNQVLVSFGDED
                     KTRRVVAAIQEDGTCWAGITVWQGRTAMRISVSGWSTTEEDVERSLAAMLRAAA"
     gene            complement(15080..>15757)
                     /locus_tag="VFZ86_14420"
     CDS             complement(15080..>15757)
                     /locus_tag="VFZ86_14420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018385557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5643512.1"
                     /translation="AFSAANVTSLLMSFGMFGSIFLLAQFFQVVQGYSPLEAGLRTLP
                     WTAMPIFVAPIAGLFSDRIGARPLLVAGMALMAGGLAWIAVVTSPTVAYAQLVPAFVM
                     AGVGMSLYFAPVANLVLSTVRRKEEGKASGVNNTIREVGGVFGVAVLASVFSAVGGYV
                     SPQAFVDGMTAAVWVGALAVALAGVAAIAIPPREDVVPDSVSLLGGGDRESSAATELA
                     CEPDAAA"
BASE COUNT         2406 a         5629 c         5542 g         2180 t
ORIGIN      
        1 catccctgaa gaaggggatg ggccgcggcg agcgccttgc gctcgccgcg gcccagaggg
       61 gccaacggca tcccgggcgc caggaggaag gcgctgccgg gctggcgggt cacggctcgg
      121 tgggggcgca ccgtacagca gccggaccct tacgacggtc gggggggcaa ccgtctcagc
      181 cgtttgtggc ttgagcacac tttgacaagt gaatatgaag cctgtgttca gacgaggttg
      241 agcgaggtta gggcagcggc tcctcgccct gctcctgccc gtacctgcgg aggaagtcgt
      301 cataggcgtc gtcgacgacc ccttggtcgg gtgcggactc ggcctgcggc ggagcctgct
      361 ccggggcgtc gctctcgccg tcttccgcgg ccgtcggtcc ttcgtcctcg gccgccgcct
      421 cgtcgctcgc gatcacctcg gcgccgggat gctcgcgctc gaacgccaac tgctcgagcg
      481 ccgcccgcgc ccattcgccg ccgaccgggt cgtcgcgcgt gaccgtgagg tccggctccg
      541 ccgccccacc gagcgcgacc agcgcatcca gggcggcgcc cttgatctgg tcgtcgccgc
      601 ggccacacaa cacatcacac aggcctgtcg cggccgcgcg ggggtccatc gcgtcgagcg
      661 tctccgccgc gcgctccggc gcggggccgt ccggatcgta gacgtcgccg agcaccgcct
      721 cgagcagcag ttccacgacg cccggaccgc gggcagcgag cgccacctgc gcctcgtcga
      781 ccagcggata cgtctcgacc gtcagcggca ggtcgagcac ggcgagcagc gcctcgctcg
      841 gcgccgtgtc gcggaactcg gacaggtact ccgcgatgga atcgcaggcg tagtcctggt
      901 cctcgcgcgt gcgcgcctcg gcgtaggcgt cggtgagctg gcgcgcgaga ccgatgagtg
      961 cttcgtcgtc gctgcggtag ctgcccatgg cagcatggta cgcgccgccg ggcctgtggg
     1021 gaacacccgg cccggcgacg tcactccccg tcgtcgaaca gatcctcgta ccgggcgtag
     1081 ccctcggccg cgaggtcctc cctcggcacg aaccgcaggg cggcggagtt catgcagtac
     1141 cgctgccccg tcggcttcgg cccgtcgggg aacacgtggc ccaggtgcga gtcgctcgcg
     1201 gcgctgcgca cctccgtgcg cgcgctgaac agccggcggt cctctcgggt cgacacagcg
     1261 tcctcgcgga gcggccgggt gaagctcggc cagccagtgc ccgagtcgta tttgtccttg
     1321 ctgctgaaga gcgggtcacc gctcaccacg tcgacgtaga tgccgtcgcg gtgctcatcc
     1381 cagtactcat tggcgaaggg cgcctcggtg ccctcgcgct gcgtgacctg gtactgcagc
     1441 ggagtcagct tcgcgcggag ctcagcgtcc ttctgcttct cgtcgctcac aggggcctcc
     1501 cccggatcac ttcccatacc gcgggcgccg cgaggagccc gccctgcgcc gtgtattccc
     1561 ggcagggcgg agagtccacg cgtcggcgtg cgcggacggg gtggctcagt gcgccgcggt
     1621 ggcgtcgccg cccacgggta cgagctcggc gtcgatgaag gcctggacca ccgagcgcac
     1681 atcagaccgg tcgtcgccgg caaggacgcg tatgcctgcc tggatgcagt gcaggagggc
     1741 cttccgcccg atgccggcga cgatgacgat gtcgacgagg ttctcaccga gcgtgaggat
     1801 cggcgtgagg ccgtggtcgc catgacgctt cggattcgtc acgacgcgta cgtcgacgac
     1861 gacgtcgtcg ctgaggtcga cgatcgtgaa gcaggggctg cgacgaaagt gcccggagcg
     1921 cggggcgtcg agcccgccgg gcaggtccga cgggatcgcg agccggagga tgtgctgatc
     1981 gtgctggttc atcgcctcga gtggttcgtg gctgcggaga cgcaagccta caccgcccca
     2041 tggacgcgtg caggtgcctt tctcggctcg gcgggctatc ctgaaatcga cgcgtcactt
     2101 gtccacgagc tcaaggggtc cgccaatgcc gctcggcggc tactgcaacg tctgtcagcg
     2161 ctgggtctgg ctgacgccct acggcgagtg ccagaacggg catccggccc tcgccgtcaa
     2221 ggacgtccag cagctcaaac cgcagtccga gccggacgtg ctcgtcggcg aggtcgtcga
     2281 cgagagcaag ccgcgcgccc gctttcgctg gtggtggcgg cactcggtgt ggatcctctg
     2341 gatgttcacg ctcgggttca tgaactggct cggcttcgtg tacgtgggcg tgcgcgccag
     2401 gcacacgccg tggacgctgt acgggttcgc ctacctcgtg cccttgctgc tgacgatcac
     2461 ggccgtcgcc acggcgctcc cggtgtggcc cttcttcctg ctgcagctgc tcgtctcggt
     2521 actgacggtg gcgcacgcgc tgtccatacg tccctactac cgggcgatca tgttcggtga
     2581 cgcatcgccg aagtcgttgc ccgcggcgcc gcagccgccc gtcctcgcgg ccggctcgcg
     2641 gccggagctg cccaggaaca tcgacgagcg cgccgccgac gtgctgcagc cggcgcgccg
     2701 ccaggtggac aagatcctcg gcacggcgag ctccatcggc aagccggccg tgcgcgacga
     2761 ggtggtcggc ctctgcacca ccgccgacca gatcatcgcc gagctggcct cccagccgcg
     2821 caagctcgac gccgcccgcg ggttcctcac ctactacctg gatgccgcgc agcgcatcgt
     2881 cgagggctac gtgaacctcg cccggcgcgg cggcacggcg ccggagatca gccagacgct
     2941 ggcgcgcgcc gaggcctccc tcaaggtcgt gcaggaggcc ttcgaccgcc agctggcgaa
     3001 cgtcctcgag gaccgcgcca tggacctcga cagtgagatc gagctgctgg agcgcaccgt
     3061 ccgcagcgag accatgtaca cccagacgag cggggagctg cgatgagcgg tctgcctgcg
     3121 gtggcccgct cgtgtacgcc ggaagaggag gcgtcatgag cgacgacatc cagaagcagg
     3181 acgagccgac gagctgggga accggctcgg atgtcgagga ccaggcggcg cccgacgtgg
     3241 ccggcgtgcc ggccgtgcgc gacgcagccg gcgcggaccc ggcggccgtc gccggcgacc
     3301 agccggccgc cggcccactc tcaccggccg acctccagcg agtcgccgag atcagccggc
     3361 aggtcgacgt gggggacacg caggccgtgc tctcctacgg cctcccggcc cagagccgga
     3421 tcgccaactt cgccgactcc ctgctcggcg acgtgcgcaa caaggacgcc ggcaccggcg
     3481 gcgaggccct gcaggacctg ctcaagaagg tgcgcgagat cgacgtcgac gccctcagcg
     3541 ccggcaccgg caaagcgaag atcccgatcc tgggccgctt cgccaacacc ttcgagcgct
     3601 tctcggcgcg ctaccagaag gtggcgacca gcatcgagaa gatcatcgac gccctcgagc
     3661 gctcacgcat ggggctgctc aaggacatga ccgtgctcga caagatgtac gagctcaacc
     3721 tcgactacct caagcagctc gacgtgtaca tcgccgccgg cgagcaggcg ctgcaggagc
     3781 tgcacgccgt caagatcccg gcgctcgagg ccgaggtcgc ggccagcgac gacgtcatgg
     3841 cggcgcagcg gctggccgac ctgcagcagg cggcgaccag gttcgagcgc cgcatccacg
     3901 acctcaagct cacgcgcatg atcgccatcc agacggctcc ccagatccgc ctcatccaga
     3961 gcaacgacca gaacctcgtg gagaagatcc agagctcgat cctgacgacg atcccgctgt
     4021 ggaagaacca gatcgtcatc gccatcagcc tgtaccgcca gcagaaggca atggaactgc
     4081 agaagcaggt gacggacacg accaacgagc tgctggccaa gaacgccgag ctcctccgcg
     4141 acggctcggc gaaggtggcg cgcgaggtcg agcgcggcgt ggtcgacgtc gagacgctgc
     4201 ggaaggtgaa cgccgacctg atcgccacgc tcgaagagac gatccgcatc caggaggagg
     4261 ggcgcgccaa gcgcgtcgag gccgagggtg agatcgggcg cctgcaggtg gagctacggc
     4321 agaagctgat cgagctccgt tcccaggcgc ctcagatcgg caccggcccc ggcgagccga
     4381 ggcggctgac cgactactga gtcccggtca ccagccggcc gtccccgggc cgcccttgaa
     4441 ggggccctcg atgtccgcgg tgatccagcc gccgtagaaa tcgcccgcct gcgccgcgac
     4501 ctgctcgccg tcgacgtagg cggcgtcgac ccggccgggg tagaaggcga cgtggtcgcg
     4561 cagcgccgtg tagcgcgcgg tgggctcggg gtagtaccag gccacgtcgc ggacctcccg
     4621 gccgccggcg acgaggtcgt agtaactcgc ctcgcccttg aactcgcacc aggtgcgccg
     4681 ccggctgggg cgcaggaagt cactgcgcac gtcctccggc gggatgtagt agacgggcgg
     4741 gtggctcgtc tcgagcaccc ggtacgcgcg cgtggtctcg gcgatcacct cgccggcgaa
     4801 ctcgacacgc accagcttgt ggctcgcctc cacgcgcggg ggtcgagggt agtcccagac
     4861 gcattccatg gccggcctca tgcctcgggc gcgccgatgg cgccgcagag ctcctcgatg
     4921 taccggatga ccccgcgtcg cgcgccggga tccagccggt cggagatcgc gaccacctcg
     4981 tcggccatcc cgtgcacctg cgtgaagatg cggtcgcgac cctctccggt gagccgaacc
     5041 acgatccgcc gccggtcgct cgggtgaggc tcgcggacga tgaactcccg ctccgcgagc
     5101 ctgtccagca tggccgtcat cgcgcccgtg gtcatgtgca ggcgacgggt aagctccgtc
     5161 gggccgagag acccggccac agagaggtgc gcgagcgcca gcagttccgc cgccgagagc
     5221 cccagccgct cgctcatctc gaggtgcatc tgcgacatca tgaggtcgag gcgccgcacc
     5281 gcgagcgcga cgtccttcgc cgaatactcc tgctcctcgc tcacggcagc tcctcgttca
     5341 cagagtcact ttatgcataa gcaactttat cagggccgtc aaggcgtcat gccccgcatg
     5401 tgcaggctgt ccgcactgct agactcgtag cgaccacgtc gcccctggga ggatgagcac
     5461 atgactgact cggaccgcgc cgcctacacg gtcgcgcaca tcgatgacat gccgttcgaa
     5521 gagggctcgt cgccgggtac ggagtggaag cccgtccgcc gcatcttcgg catcggctca
     5581 ttcggcaccg cgctcgcgcg tgccacggcg gcgggcgaca cgctgacgca cgatcacgac
     5641 gaggtagctg cgcgtcacga ggagctcttt ctgatcgtca gcggccacgc cacgtaccgg
     5701 gtcgacggcg aggagatcga cgctccggcc gggaccttcc tctacgtccc ggatccggcg
     5761 accgtgcgcg gcgccgtggc ggtcgaggcc gggaccacga tgttcgtcgt cggcgccgag
     5821 cccggcgcgt cgttcacacc ctcggactgg gacaccgtgc cgctgccgga gtgaaggacg
     5881 caggcggccg tagcctgagc cggcgccgtg tcgccggacc tcaatcgtcc gcctctgcca
     5941 agaggccgtc caggtccagc ggctcgacga ccatcgcata gctgccgtcc gggttgaccg
     6001 gccacccgtc gcggggccgg tcccagtaca gctcgatgcc gttgccgtcg gggtcgtgaa
     6061 ggtagatggc ctcgctcacg gcgtggtctg acgcaccctc gatggggtag cgtcgctcga
     6121 tcagtgcgcg cagcgcgcga gccagcgccg cccgcgtcgg gtagcggatg gccacgtggt
     6181 agaggccggt cgccgagcgc ggcggcggag agccgccgcg cgactcccag gtgttgagac
     6241 cgatgtggtg gtggtaaccg ccggccgaga cgaacgccgc ctggtcgccg agacggccgg
     6301 tgagctcgaa gcccagcacg ccctggtaga acgcgagtgc gcggtcgagg tccgagacct
     6361 tgaggtggac gtggccgatg ccggttccgg cgtcgagcgt cggggggtcg agcttgagag
     6421 gttccgttct ggtcgccatg aggtgctccg agtgatccgg gggtgatgga aggacggcgc
     6481 ttcgccgtca ggctccggcc tcgccgggtc cgcgcagcgc gcgtaacgac gcggtgagcg
     6541 agtcgagctg ctccgcagag agcccgccgg cgagttgctc cgccactccc gccgctacgg
     6601 cgggcgcagc cgacgcggcc gcccggcggc cctcgtcggt gaggcccgcg tacacgacgc
     6661 gccggtcttt ggggcaggcg gcgcggacga cgagcccacg gtccgccaga cggtcgacga
     6721 gccgcgtgac gccgctcttg ctcagccgca gcgaccggct gacgtccgcc atgcgcaggc
     6781 gctgctcggg cgcctcatcg agccgcgcca gcagctcggc ctcctcgggc agtacgccca
     6841 gttcggcgtc gagccggtgc tccaaccgcg cgcgcacggc gcccgtgaag gatgtgagct
     6901 ctgtccacag ctccatggcg gcgcgctcgc ggcctgcatc tgtcggcgtc tgcttcatgc
     6961 tttgggtatg cccatgtttc agctggaata gtcccggaca gaactattgt cgatgcaaat
     7021 atctcactgg acggcgccgc acaccggagc cgggaccgga ggacaccatg aagatcgcca
     7081 tcatcggagc aggcaacgta ggcacagccc tcgcaggggc ggccaccaga gcggggaacg
     7141 ccgtgaccgt gagctcgacg tccggcgagt ccgcccgggc tgcggcggac cagaccggcg
     7201 cccgggcggc ggcctccaac aggacggcgt tggaggatgc cgatctcgtc atcctggccg
     7261 taccctacgc ggccgtggac gacgtgctcg ccagcgtcgg agacgcgatc tccggcaagg
     7321 tcgtcgtgga cgcgaccaat ccgatcaagc ccgactactc gggcctggcg accggcgaga
     7381 ggtcgggtgc cgaggagatc caggccaagg cgccgggcgc ccgcgtcgtg aaggcgttca
     7441 acaccgcgtt cgcggcccgg caggccgacc ccagagtggc cggcggtctg cgcgtcgacg
     7501 gctacgtcgc cggcgatgac gaggccgcca agacggccgt gctcggtctc gcggaggcga
     7561 tcggcttcaa ccccgtcgac gccggcgggc tgacgatgtc ccgccacctc gagggcatgg
     7621 cctggctgaa catcagcctg cagatgcagc acggctgggc atggcaggcc ggctggaagc
     7681 tcgtcggccc cgacggcgac gcggcgtagg ccacgccccg cgacgcaccg tcgaccggca
     7741 taacggtccg tacggcacga gggcggggcg gtcgcacgac ctccccgccc tcctcgtccg
     7801 tccctcaggc gtcggccgcc gagagcgccg gcgtttcaca cgtgccgagc acctcgtcgc
     7861 acgacggctc gcggtcgtga cgcgccgagc gcagacggaa gcgcccgggc gtccacgtgg
     7921 tgacgaccgc caccacgaaa ccactcaggg cgacgacgcc ggcgatcacg agccccgtcg
     7981 agaagcccac tgtgagcccg tggaagagga tcgcctgcgg tacgccagcg gcgccttccc
     8041 cggcgccggc gacagacccg ccggcgccct gcgcggcgcc gctcgccgcc gccgcggcca
     8101 tcgagtcgcg gaccgtgttc gaaacggagg tccaggcgat ggtcccgatg gctgcgaggc
     8161 cgatcgagcc gccgacctgc tgcccgatgt tcagcaggct ggaggcggcg ccggcctcgt
     8221 cgtgccgcac gtgtgagacc accatcagcg tcagcggcac gaacatcagc cccatgccga
     8281 gcgcgaggac gagcatgggc gccaggaggt cggtccagta gctcccggtc gggctcagcc
     8341 gcgacagcaa catgaacccg gctccggcga acaaggggcc ggccatcagc aacgggcgca
     8401 cgccgacctt ggccaccagc acgcgcgcga cgaagacgtt gatcgcgatc aaggcgatcg
     8461 ggaagggcac ccaggcgagg ccggccctca cagggctgta gccccagacc gtctgcacgt
     8521 agatggtcag gaagaagaac acggcgaaca tcgcggtgcc gagcagcaac atcatgatgt
     8581 aggcgccgga gcggcggcgg gagcggagca ggttcaggtc gagctcgggc tccctgacgt
     8641 acctctcgat gaagacgaag ccgacgagca gagcgaccgc cccggcgagt gacgcgagcg
     8701 tcacccggtc gccccagcgc gacgcgccgt cgggtcccgc ggcggcgtgc gtgaggccgt
     8761 agaccagcaa cgccaggccg gccgtgctgg tcaccacgcc ggggacgtcg aggcggcggc
     8821 gcaaccggcc gccgcccgcc agcgccatcg gggccagcgc ggcgacgacg agcccgatcg
     8881 gtgcattgac gaagaagacc cagcgccacg acacgtacga ggtcaggatc ccgccgagca
     8941 gcagaccgat ggcgccgccg gcgccggcca tgcccgccca gacgccgagc gcccggttgc
     9001 gcggtgcgcc catcggaaac gtcgtggcga tcagagcgag ggccgtgggg gctgcgagcg
     9061 cggccccggc gccctgcacg gcgcgcgacg ccagcagcca ccagcccgtg gtggcgaagc
     9121 cgccgaccag ggaggccagg gcgaacagca ggacgccggt catgaagatg cgccggcgac
     9181 cgtagatgtc gcccagccgg cccccgagca gcagcagact gccgaaggcg agcgagtaag
     9241 cggtgatgat ccactcgagc ccggtcccgc tgaagccgag cgcctgctgg atgtgcggca
     9301 gcgcgacgtt cacgatcgtc gcgtcgagga cgatcatcag ttgggccgcg gcgatcacga
     9361 cgagcgccag gccgaggcgg cgcgggacgt gcgtcgcgcg gtcggtggtg gccgcgtctg
     9421 actgcggatc gatgtcggcc acagcgtcct gcggccgggg agagaggtgt tcgctcatgg
     9481 gacggctccc agggacggag ggaatgcgga cggtttcagt ccacctaccg cgcgagcgga
     9541 tcgtcaaaca tcgaggctgg cgggccagcc agacgcgacg aggcaccctg agtggtcgtc
     9601 aggccacccg gttcagctgt cgcgcggcaa ggtgctgctg cacgagccgg acggcgacgg
     9661 cgccctcgcc gaccgccgac gccacgcgct tgacggagtc ggcgcgcacg tcgccggcgg
     9721 cgaacacgcc ggggaggctc gtctcgagcc ccagcggcag ccgctcgagc ggccacggct
     9781 caggcagccc tgcgccgagg atgtccggac cggtgagcac gaagccgtgg gcgtcgcgcg
     9841 ctatctcggg cggcagccac tcggtgtgcg gatcggcgcc gatcatgacg aagagggcgt
     9901 cggccgggac gacctcccgc tccccgctgt cgatctgctc cagcgtgagt cgccgcaacc
     9961 gggtgtcgcc gtctccgtcg acgatgaggg tcctcgggcg cagagagatc cggtcgacgt
    10021 tactcaggaa ggacacgagg tagtgcgaca tcgtgtccga gagcccgtcg ccgcgcatga
    10081 cgagggtgac gtgacgagcg tacttggcga ggtggatggc cgcctggccg gccgagttgc
    10141 cgccgccgac gatatacacg tccatgttga ccagcgatct cgcctcggac gagaccgacc
    10201 cgtagaacac gcccgagccg cggaaaggat ccagccgggg caccgccagg cgccggtagg
    10261 tgacgcccgt ggcgaggatg accgcgccgg cacgcagcga cgcgccgtcc gcgagctcga
    10321 tcatgtgcgc gtcgccgtcg gggcgcagcg aggtggcctc gttggagaag aggaactcgg
    10381 ccccgaactg ccacgcctgc tcgtaggcgc ggaacgtcaa ttcgtcgccg gagatgccgg
    10441 cggggaagcc gaggtagttg tggatcatgg tgctctgccc ggcctgcccg ccgagcgcct
    10501 cgcgctcgag caccaccgtg ctgaggcctt ccgaagagcc gtagacggcg gcggcgaggc
    10561 cggccggacc agccccgacg acgagcaggt cgcactcgtc ggcggacggc cggctgcgca
    10621 cgccgaggga ctccgagacc tcgcggttgc tcgggtcgac aaggacgcgg ccaccgaaga
    10681 cgacggccac cggcagcttc ggcggcgcgt ccaggccggc gtcccgcagg atctcccgac
    10741 cctccccgga gtcggcgtcg tagaagccgt aggcgacgcc gttacgcccg agcagatcgc
    10801 gcagctcgtg cgaacgcggc gcccagcgct ccccgacgat gcgcacggcc tcgtacaccg
    10861 gtcgctgcag gcgggcccac tcgtagagga agcccgccac ctcactgtgg aaacgctcgt
    10921 ccgtcgccgt gtgccacggc ttggtgatga agaactcgat ctggccgacg gcgcaccccg
    10981 tcaggatcgc ctgcggcgcg ctgcggtctc cccacgagac gagcatgacg cgcttggcga
    11041 ccgggtcgat cacccgcgcc tccccgagga agtcggggcc ggacatctcc ggcatcacct
    11101 ggtcggcgag gatcagggcg accggctggc cctcgatctt gaggcggcgg agctcgtcga
    11161 gcgccgccgc cgccgactgc cgggcgagca cccgatagtc cctgccgtac cgcttgcgca
    11221 actcctgctc cgtgatcgtc aggctcgtca tgtcgtcgtc cacgacgagg atgaccggct
    11281 gtaggctcat cgtctcctcc cgggctgccc catatcgtct ggttgcccag ttgtcgccat
    11341 ctgatgcccg ccggcgttcg aacccccgac tgcggagcgt cagctcgccg ctgcgagcga
    11401 cgccctcacc tcgaaggtga acatcatgcc gccttcgagg tgctcggcga tgtggcagtg
    11461 caccatccag gagccggggt tgctgcactc cagcaggatg tccaccgtct ttccggtcgg
    11521 gaccagcacg gtgtccttcc acatgatgct gggagccggc ccgccgtccc agcggagctc
    11581 gtagaaccgt tgcccgtgga agtgcacggg gtggtgcatg gggtggtcgg agtcggggtc
    11641 gttgatgatg cggaccttga cgcggtcgcc ctgcgcgaag acccagtgga tgccctcgtt
    11701 gtccgcaccc agggcctcgt cgtggatgcg ccacaccatg ctctcgggag aggaggacgc
    11761 gttgtgctcg ggcatgtcgt cctcccactc gatgagcgag tgagccgcgg gcgtggagcc
    11821 ggagtgctcc gtgccggcgc gcgccatacg gccatggtcc atcgccatcc ccggcatgtc
    11881 gccctgcagg acgaggaccc tgtcgggctc gcgctcgagc tcttcggtcc agcgagcccg
    11941 ttcctccgcg aactccgggt tgcggcgcag tcgcccgaac tccccggaga gatcgggcag
    12001 ggcctcctcc gcctccacga cgaacgccgc cagccgatac gtcctgccct cactcacgtg
    12061 ctcgagtacg tactccccct cgtccgggaa gaacgcgtcg accacccagc gctcggacgg
    12121 cgcgaggatc aggcgctcga ccatccgctc ctcctcgatg cgcccgaggt ccgagccgac
    12181 gagcttgagg cgcgcgcccg ggatgcacaa acggaacacg cgcgtgttgg cggtgttggt
    12241 gaggtacaac cgcacgacct cgccccgccg cgcgacgaac cgcggcgagg tctccccgtt
    12301 gaccagcatc acgtcgccga accggcccat ggccgtgtgc gacgacccct gcacgctgaa
    12361 gggcgcgacc gcgccgtcct ggatcaggat gtcgtccacg atgaggggga ggtcgcggtg
    12421 ggccgcgggc cagtagtccg ggtcctgcgg ctcaacgatg atgttgccgt acaggcccat
    12481 ctcctgggtg tagtcctcgc gcatgtgcgg gtggtaccag tacaccccgg gatcggggaa
    12541 gcgcagccgg taggtgaagt cccgacccgg ccgcaccggg tcctgcatgc cgccgtggcg
    12601 ccctccacgg ggcacgccgt cgtagaggta gtcgtgcctc aggccgtgcc agtgcacggt
    12661 ggtgtcgagg tcaagcccgt tcacgaaccg gcagacgacc tccgagccct gccgcaccct
    12721 gatcgtcggg cccgggaccg agccgttgta cgcgagcaga cggacatcgg cctcgccgac
    12781 gcgcttgcgc acgggcgagg ccttcagctc gaacgagccg ccggtcgcga ccgccagggt
    12841 ctctgccgga tgcgcttcct caagcccgct gatgctctgc gagaagtacc gtggtgtcat
    12901 cggagtacct tcctggcgct catggaggtc tcttcccggc cagccgcaac cgacgacacc
    12961 ccgaccgtca ccgcccaccc gcggcagatc acagccggac tcagcccttc gccggcgcct
    13021 ccgccaggtc gtgcgccatg cgctggtagc gcgcggtctg ggccgcggcg ccgggcgagg
    13081 aggccagccg ctcgaagtcc tcggccagga tctcctgttc ctggacgctg agcagctcgt
    13141 ctgtcagcgc gaacaggacg gtgtcctcct tgccgatgtg cagcggcagg aggaaggcgt
    13201 agagccgcag gttctcgacg atgacggcgc gcgccgcggc ggcgcgctcg ccgtccggca
    13261 cgctcacgtc cttgagcgcg cggtcgatcg cccggatgca ttcgcgcgcc gcctcgtgct
    13321 cgctgagcag cacgctgatc gtcccgccgg cggcggcgct ccgctcttcg agcagcggga
    13381 agagcaggct ctcctctttg gtgtggtgat cgccgtcggt gaagttccgc gtgaagtcca
    13441 ccatctgggc gacacgcacc acatcgacct cgccgctccg ttcgatgcgg gaggcctccg
    13501 cctccatggc gccgacgacg agcagcacgt attcgtgctc gtcggcgagc tgtcgcgtcg
    13561 ggctgtcggg catggcgtct ccttccggtg tctctaccgg attgcccgcg atgcggcgac
    13621 ccctaacgcc gccggcagtg tccggccgcc tatgcggccg cccgaagcat cgccgcgagg
    13681 ctccgctcca cgtcctcctc cgtcgtcgac cagccggaga cgctgatgcg catggcggtc
    13741 ctcccctgcc ataccgtgat acctgcccag caggtaccgt cctcctggat ggccgcgacg
    13801 acgcggcggg tcttgtcctc gtcgccgaac gagacgagca cctggttcag cacgacgtcg
    13861 ttgaggatct cgtacccggc gtccgccagc ccccgggcga agctcgccgc gtgccggcaa
    13921 tcccgctcga ccaggtcggc caggcccgag cggccgagcg ccctcagcac cgcccagacc
    13981 tccacgccgc gggcgcggcg cgacagctca ggcgcgtgcg ccgccggctc gcgcatcatg
    14041 ctgtccggca ggtacgcggc ggtgaggccc atggccgcgc gcagcggctc ggggtcgcgc
    14101 acgaacgcga gcccgctgtc gtagggggtg ttgagccact tgtgcgcgtc ggtggcccac
    14161 gagtcggccg cccccacgcc gcgcaccagg tgcgcgcggg caggcgccac ggccgcccac
    14221 agcccgaatg cgccgtccac gtgcacccag gccccggcct cgtgggcccg ggcggcgatc
    14281 tcctcgaccg ggtcgaaggc gccggtgttc acgttgccgg cctgcgtaca cacgatcgtc
    14341 ggacccgaga tcggcggcag cctgtccgcg cgcatgcgtc cctggccgtc gacctcgacg
    14401 cggcgcacct gctctctgcc gagcccgagg agcccgaccc ctttgagcag cgacgggtgc
    14461 acctcgtcgc cggcgatcac ggtgaccggc ggcgcgccgc cgaggccctt ggcgtccacg
    14521 tcccagccgg cgcgggcgag gacggcgtgg cgcgcggcgg ccagcgcggt catgttggcc
    14581 atggtgccgc cggtgacgaa gcccgcgacg gtggtggacg gcaggccgaa gagctcgacg
    14641 agccaccgct gcgccacctg ctccagagca gccgccgtcg gggacgccgt ggaggcgccg
    14701 gcgttctggt cccaagcggc cgcgagccag ctggcggcca cggcgaccgg gtgcgagccg
    14761 ccgatgacga agccgaagaa cctggggccg gctgacgcca tggtcgccgg cgagccggcc
    14821 tcgtcgagca gggcgatgac ccgctctgcg tcgtcagggt gctcgggcag cggctcgtcg
    14881 aagcgctcga ggccggtgat cgcgtccggc gacggcgcga cgcgacgctc gggcaggtcg
    14941 cgcaggtagc ggatcgcccg agcggcggtg tggtgcagca ggtcttccat gggtgtggcc
    15001 cccggcgtcg tgaggtcaga ctgatgcgca gttccctccc gattccccgg ggcgaagcgg
    15061 tgaaacgctc agaccggcgt caagccgccg cgtccggctc gcacgccagc tcagtcgcgg
    15121 cactgctctc gcggtcgcca cccccgagca acgacacgga gtcgggcacg acgtcctcgc
    15181 gcggcgggat ggcgatcgcc gccacgccgg ccagcgcgac ggcaagagcg ccgacccaga
    15241 ccgccgcagt catgccgtcc acgaacgcct gcgggctcac atagcctccc acggcgctga
    15301 acacgcttgc cagcaccgcc acgccgaaca cgccgccgac ctcgcggatg gtgttgttca
    15361 caccgctcgc cttgccctcc tccttgcgcc gcacggtgct cagcaccaga ttggcgaccg
    15421 gggcgaagta caggctcatg ccgacgccgg ccatcacgaa cgcggggacg agctgcgcgt
    15481 aggcgaccgt cgggctggtg acgacggcga tccacgccag ccctcccgcc atcagcgcca
    15541 tgccggcgac cagcaacggc cgggcgccga tgcggtcgct gaacaggccc gcgatgggcg
    15601 cgacgaagat cggcatcgcc gtccacggca gggtgcgcag ccccgcctcg agcggcgagt
    15661 acccctgcac cacctggaag aactgcgcca gcaggaagat cgagccgaac atgccgaagc
    15721 tcatcagcag agaggtgacg ttggccgccg agaaggc
//
LOCUS       DASUMX010000306         2064 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1842930, whole genome shotgun sequence.
ACCESSION   DASUMX010000306 DASUMX010000000
VERSION     DASUMX010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1842930"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..909)
                     /locus_tag="VFZ86_14425"
     CDS             complement(<1..909)
                     /locus_tag="VFZ86_14425"
                     /inference="COORDINATES: protein motif:HMM:NF012800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEX5643513.1"
                     /translation="MTHDDTALRAFGADVQRLIEGQDLTRERTYELFRQVLLGEQPDL
                     QQGALLAALVAKGETPEEIAGAWQAIVEFDTVPAAIASVAGAAQQGPLVENSGTGMDT
                     LKTFNVSSAAAIVAAARGARLARHGARALTSRCGAVDILEAVGVDVDCDVTAVERSIG
                     LAGIGLFNGMSPAIHPGGLGRILSQIRFGSTLNIAASLANPVRPTHGVRGVYAEGLLG
                     PVGSVMMEIGYEHALVVHGYDDRREGGMDELSVLGATVVSELRADGRRDSYTVEPEDF
                     GLRRAAHGEIAPLGDLRSEAVRFLQVL"
     gene            complement(906..1967)
                     /locus_tag="VFZ86_14430"
     CDS             complement(906..1967)
                     /locus_tag="VFZ86_14430"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX5643514.1"
                     /translation="MQAVVIREVGAFEVTDLPAPEPGTGEALIRVSVTGLCRTDLKIV
                     RHGHRDLTLPRVPGEEVVGEIVRLGPHDTSAPQGSEPFAPGDRVYVYPGVWCGECPSC
                     RRGAENLCRQMRIMGFHRDGGFAEYVAAPLQSLIRVPAGLGDEEAVFAEPLSCCLNAL
                     ELGGVGEGDVLGVWGAGPAGTLVSRAGALRGAAVAVVEPDVRRREALTSAPGSPPEVT
                     AHRCAPGLVDVAVVAVGDRRAYEAALAALAPRGRLVVFSGLLPADDAALRVSLSAIHY
                     HEHTLVGAYGCSFRHGVEALDLLASRRLRVDDLISHRLRLAELDEGLGLVARRAGMKV
                     HLYPGPLPSRPRPSREEPR"
BASE COUNT          273 a          762 c          766 g          263 t
ORIGIN      
        1 cagcacctgc aggaagcgca cggcctcgga gcgcaggtca cccagcggcg cgatctcgcc
       61 gtgcgccgcg cgccgcagcc cgaagtcctc gggctcgacc gtgtacgagt cccggcggcc
      121 gtcggcgcgc agctcgctga cgacggtcgc gccgagcacg gacagctcgt ccatccctcc
      181 ctcgcggcgg tcgtcgtagc cgtgcacgac cagcgcgtgc tcgtacccga tctccatcat
      241 cacgctcccg acgggtccca gcagcccttc agcgtagacg ccacgtacgc cgtgcgtcgg
      301 ccgcaccggg ttggcgagcg acgcggcgat gttcagcgtc gagccgaagc ggatctggct
      361 gaggatgcgt cccaggccgc cggggtggat ggccgggctc atcccgttga agaggccgat
      421 gcccgcgagg ccgatgctgc gctccaccgc ggtgacgtcg cagtccacgt cgaccccgac
      481 ggcctcgagg atgtccaccg cgccgcatct ggacgtgagg gcgcgcgcgc cgtgccgcgc
      541 cagacgcgcg ccccgagccg cggcgacgat cgccgcggcc gaactcacgt tgaacgtctt
      601 gagggtgtcc attccggtgc cggagttctc gaccagcggg ccttgttgag cagcgccggc
      661 gaccgacgcg atcgccgccg gcaccgtgtc gaactcgacg atcgcctgcc aggcgccggc
      721 gatctcctcg ggggtctcgc ccttggcgac gagcgcggcg agcagagcgc cctgctgcag
      781 gtccggctgt tcgccgagca ggacctggcg gaagagctcg tacgtccgct cgcgggtcag
      841 gtcctggccc tcgatgaggc gctggacgtc ggcgccgaaa gcgcggaggg cggtgtcgtc
      901 gtgggtcatc tgggttcctc tctcgatggg cgcggccgtg agggcagcgg tcccggatac
      961 aggtggacct tcatgccggc gcggcgggcg acgaggccga ggccttcgtc gagctccgcc
     1021 agccgcaggc gatggctgat gaggtcgtcg acgcggagcc ggcgggaggc caggaggtcg
     1081 agcgcctcga cgccgtgacg gaagctgcag ccgtaggcgc cgaccagggt gtgctcgtgg
     1141 tagtggatcg cgctgaggct gacgcggaga gcggcgtcgt ccgccggcag cagcccggag
     1201 aagacgacca ggcggccgcg cggcgcgagc gccgcgagcg ccgcctcgta cgcccggcgg
     1261 tcgccgacgg cgacgacggc gacgtccacg agcccgggcg cgcaccggtg ggcggtgacc
     1321 tccggcggag accccggcgc gcttgtcagg gcttcgcggc ggcgcacgtc cggctccacg
     1381 acggccacgg ccgcgccgcg gagggcgccg gcccggctga cgagcgtgcc ggccgggccg
     1441 gcgccccaga cgccgaggac gtcgccctcg cccaccccgc cgagctcgag cgcgttcaga
     1501 cagcaactga gcggctcggc gaagacggcc tcttcgtcgc cgagcccggc cggcacgcgg
     1561 atcaggctct gcagcggcgc cgcgacgtac tcggcgaacc cgccgtcgcg gtggaagccc
     1621 atgatgcgca tctgccggca caggttctcg gcgccgcgcc ggcagctggg gcactcgccg
     1681 caccagacgc ccgggtagac gtagacgcgg tcccccggcg cgaacggctc ggagccttga
     1741 ggagcggacg tgtcgtgcgg cccgaggcgc acgatctcgc cgacgacctc ctcgcccggc
     1801 acccgcggca gcgtgaggtc gcggtgcccg tgtcggacga tcttgaggtc ggtgcggcag
     1861 aggcccgtga cgctcacccg gatgagggcc tcgcctgttc cgggctcggg cgccggcaga
     1921 tcggtcacct cgaacgcgcc gacctcgcgg atcaccactg cctgcatcgc gccgcctttc
     1981 ggtctccaaa cgcacagaag cccgggccgt cgcactcgtc tggcccgggc cccccgtttt
     2041 cgaggccgca gcgcgcgtcg ccgc
//
LOCUS       DASUMX010000307        15494 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_407384, whole genome shotgun sequence.
ACCESSION   DASUMX010000307 DASUMX010000000
VERSION     DASUMX010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 15494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15494
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407384"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            112..318
                     /locus_tag="VFZ86_14435"
     CDS             112..318
                     /locus_tag="VFZ86_14435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643515.1"
                     /translation="MANAGSRNRRLGPPYPTESARACNSCVGTTDGHADIHGANERVL
                     QDQSEKATPAEADFFGRLATGGAR"
     gene            407..1270
                     /locus_tag="VFZ86_14440"
     CDS             407..1270
                     /locus_tag="VFZ86_14440"
                     /inference="COORDINATES: protein motif:HMM:NF015669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HEX5643516.1"
                     /translation="MKSRRSIAAGRRRRRPARGRRTRLVVFIAVAGLAAAVPFAASAC
                     GSDDSTDSSVQDAAALGGRIWKAVEIAGVTTAPADGADATAAFEGGVVSGSGGVNRYT
                     AAYTTEEPDGITIAQAAATLMAGPEEAMAQEQAYFAALAQAATFSVDEGSLTLMDDQG
                     ATLVRFEVAAPRALTGTTWQALAYNNGRGALQSPAIDSSITAVFDEDGNLGGNASVNQ
                     YSTAYSTTGETMSIDAQIITTKMAGPPELMEQEAAYLAALPQTATYTVEGDELWLRDA
                     EGAALAHYVAE"
     gene            1329..2600
                     /locus_tag="VFZ86_14445"
     CDS             1329..2600
                     /locus_tag="VFZ86_14445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HEX5643517.1"
                     /translation="MSETPVPAFHVMAKPTGARCNLRCDYCFFLEKEELYPGSDFRMR
                     DEVMEAYIAQTAQAQRVPAVTLAWQGGEPTLMGLDFFRRARAAEEGRVPAGMAVERTL
                     QTNGVLLDDEWCAFLAENDYLVGLSIDGPRELHDAYRHDAGGRPVFDRVVAAARRLQK
                     HGAEFNVLCTINAANAGHPLEVYRFFRDELGARYVQLIPIVEVETPPAGGRPGTVSAR
                     SVQPGDYGRFLNAIFDEWVRRDVGEMFVQFFDGVLAAYVRGVSSLCILQPTCGEGVAL
                     EHNGDLYSCDHFVDPPYLLGNIMETPVGDLVRSEQQRAFGRAKQETLPAFCRACAYLF
                     ACNGECPKNRILVTPEGEPGLSWLCAGLKAFFAHTDRRMRLMADILKRGGEAPEVMEL
                     LAAEARGTGRNDPCPCGSGKKYKRCCGIAAR"
     gene            2674..4161
                     /locus_tag="VFZ86_14450"
     CDS             2674..4161
                     /locus_tag="VFZ86_14450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017300996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HEX5643518.1"
                     /translation="MPNGKPNILVLWGDDIGFWNLSCNNRGMMGYQTPNIDRLAHEGV
                     GFTDFYGQQSCTAGRAAFITGQNPLRTGLTKVGLPGSDFGIRPEDPTIAELLKPLGYA
                     TAQFGKNHVGDLDEHLPTNHGFDEFFGNLYHLNAEEEPEHEDYPKDPGFKERFGPRGV
                     IHSWAQPDGTQKIEDTGPLTRKRMETIDDEVTARTLEYLDEVTAADKPFFLWWNATRM
                     HFRTHVKEENRGVSGQGAYNDGMVEHDRHVGMILDKLDELGIADDTIVMYSTDNGPHY
                     NSWPDAAITPFRSEKNSNWEGAYRVPALLRWPGKWQAGVTLNGIVAHQDMLTTFLAAA
                     GEPAIAEKCRAGYDAGGTTFKVVLDGKDMNSYFAGETDESPRDRFFYVNDDAELVALR
                     VGDWKFIFKEQRARQLAIWLDPFVELRAPKIVNLRRDPFERALDDSNTYYDWMMDRIY
                     MVVPAQAAVAEAVAELREFPPRQKPSSFNLDRVLEKLQEAGGSQS"
     gene            4226..5377
                     /locus_tag="VFZ86_14455"
     CDS             4226..5377
                     /locus_tag="VFZ86_14455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5643519.1"
                     /translation="MIPRTELRVRIDAALGRSPIVVLTGPRQCGKTTLARELLSADSE
                     NYFDLEDPASLARLDEPMTALRPLSGLVVIDEVQRLPDLFPVLRVLADRSPSPARFLI
                     LGSASGALLRQTSESLAGRAERVLMGGFSVDEVGGAEADDLWRRGGLPRSFLAATEAD
                     SVSWRSQFIQALMERDLPQWGVRVPAVALRRFWTILAHYHGQIWNATDPAASMGVGTK
                     AVRHYLDLLTDAYMIRQLQPWHANLRKRQVKAPKVYVRDTGLLHQLLGITTAAELYTH
                     PKSGASWEGFAIEQVLAGQPHDDAFFWATHQGAEIDLVLRRGERLLGVECKRVDAPRL
                     TASIRIALEDLGLERVVVVYPGERRYLISDNVEALPLHELAGAPSLFGA"
     gene            complement(5432..6064)
                     /locus_tag="VFZ86_14460"
     CDS             complement(5432..6064)
                     /locus_tag="VFZ86_14460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX5643520.1"
                     /translation="MADTADNQSLPADDRSPQAVARGVFSELVALDTAVYQAIEATPT
                     PLIDAPLRRLSNTANHAKLYMGIAALLFAAGGKKGRRAAVTGMAAVGLNSFVVNVPMK
                     FAGHRPRPERGVVRFEEARYVKMPTSTSFPSGHSASGFAFAAGVAEAIPALGTPLRGL
                     ATVVAYSRVHSGVHYPGDVIVGSLVGMAVGEGTALASRAVRKRLPGRSAG"
     gene            6113..7900
                     /gene="sulP"
                     /locus_tag="VFZ86_14465"
     CDS             6113..7900
                     /gene="sulP"
                     /locus_tag="VFZ86_14465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015661991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate permease"
                     /protein_id="HEX5643521.1"
                     /translation="MATKAEQTEAAPVKRSRLDAVVPGVGMLRAYKPAWLRFDLVAGI
                     VLAAILVPQGLAYAELAGVPPVTGLYTTVVCLIVYALFGPSRVLVLGPDSSVSPLIFA
                     AIVPLMVADDPASAIALAGMLALMVGLIEIGLGLGKLGFIADLLSKEVQVGYMNGLAI
                     TIIVGQLPKLFGFSTDADSFVDDVTAFFTNLDQTNTTTLLVGLGTLALLFGLPRISKK
                     LPAILVAVVVATVITAALGLAEKGVDTVGTLPQGLPVPSFPWTGWSDLATLAAAAVGI
                     VLVSLTDTIATSSSFAARRGDEVDADREMIAVGASNVGAGLFQGFAISVSSSRTAVAD
                     RSGAKSQVAGLVGAGLVALMLLAVPGLLKNLPQTALAAVVIMAAVSLADIAVLRRFAH
                     VRKTALLLSLVATAGVVFFGVLEGILIAAILSILLFFRRSWWPPGAVLGYVAELKGYH
                     DVERYPTAEQEPDIVIFRWEAPLFFANAGIFRQRVRKLVVERKPLWIVLQCEAITDID
                     VTAADVLRQLDEELNAKGIHFAFVELRERLQDRLRLYGLWQTLNREHVFNKMKPAIRA
                     LSEHGPYGEDGPHGPAGLRHLEPPDEPES"
     gene            complement(7963..9330)
                     /locus_tag="VFZ86_14470"
     CDS             complement(7963..9330)
                     /locus_tag="VFZ86_14470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020420113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX5643522.1"
                     /translation="MTEHTASQGPAPGRRLAAAAAIALFLGVFVATVLVVLDRPLEVV
                     VQVVLLAVVLVAAWNALTRKGAVRGTWIVVAVVAAVAVVVIQIAGSEKHLLSLLVRLA
                     LLGVALLLSRYALTRDVRSLKQTETPGKPAPAARRGVLIMNLKSGGGKAEKFHLVDEC
                     RARGIEPVVLQRGDDLLQLAHDAIDGGADVIGMAGGDGSQALVASIAAERGVPMVVIP
                     AGTRNHFALDIGLDREDVVGALDAYDEARERVIDLADVNGRIFVNNVSLGLYATIVQS
                     PEYRDAKRETTLAALPEMLGPGSKPFDLRFSTPDGREHDGAHVIQVSNGPYGTTTGGI
                     TSRPHLDTGTLGVFSLVVPDAVSATRAIAALTTAHPALYDGYLTWATPTFEVDSGSPI
                     AVGLDGEALDMEPPLVFRSRPGVLRLRLPLRAIGYSPAARATGVRGLLGLWRVVLGRP
                     VGIDG"
     gene            complement(9342..10889)
                     /locus_tag="VFZ86_14475"
     CDS             complement(9342..10889)
                     /locus_tag="VFZ86_14475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HEX5643523.1"
                     /translation="MTEDLFLGLDAGSSSTKAVLVDAGGGVVADARTEHEILRPHPGW
                     AEQDAERDWWGSVLDVSRRVLKGRGACVRAVGLSGLGPCVLPADARGRPLRPAILYGI
                     DSRAVAQAERLTAELGSDAVLARCGSRLSSQSVGPKLRWLAEREPEVWSATRRVYGAS
                     SYLVARLTGEYVLDHHSASHWAPLYDVHENDWIDEWVPAAAPGPALPRLVWPHEVCGG
                     VSPEAAAAIGIAAGVPVAAGSIDSWCEVAASGLRGPGQGLLVYGTSMFLIEVDSPARP
                     DPRLWSTVGFAPRSLNIAAGVASSGALTAWLRDIAGGPAYERLYAEAAAAGPGAGGLL
                     ALPYFAGERTPLFDPDLRGAIVGLTAAHGRGHLFRALLEAAAFAVRQNLETMEAAGAT
                     IVALRSAGGGVAGALWPRIVSDATGLAQDVRQGPSQAGVGAALLAAVSVGAATLQTTW
                     PQATVRVEPDPGHRALYDELYGWFREIAVATRPHAHALAAWQRERSRGTMSPAADGEQ
                     SGPAGHE"
     gene            complement(11107..11481)
                     /locus_tag="VFZ86_14480"
     CDS             complement(11107..11481)
                     /locus_tag="VFZ86_14480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643524.1"
                     /translation="MDGKWQGWAAAGAILMVFVGGFKAISGFIGLFNDEWLVRGFNAY
                     YFIDISALAWWYLIVGVILLLAGLAVMNGKAWGRWVGVVAAGVAIISELLWLPIYPVW
                     SILLITMYVFIMIGLVAVGSEE"
     gene            complement(11620..11994)
                     /locus_tag="VFZ86_14485"
     CDS             complement(11620..11994)
                     /locus_tag="VFZ86_14485"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5643525.1"
                     /translation="MPEKHLSCVLLADGHQALCEGVRGLLESAFGTVVMVADETSLLE
                     GARRLQPEVAVVDLSLARHSGLDWLRALRARCPEVKVIVLSVHDEQSVRQAALDAGAH
                     AVVLKRAIATDLLPAIARLRAG"
     gene            12147..14144
                     /locus_tag="VFZ86_14490"
     CDS             12147..14144
                     /locus_tag="VFZ86_14490"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX5643526.1"
                     /translation="MADVDATERSPEDEGGRLEFETLLFDLSSSFINLPPEQVDREIE
                     DVQRRVCEVLAVDLSALWEVTAAGGAPLKLTHFYSSQEDLLPPMRGMSAQEYFPWLHR
                     EMLAGRSVAVSSLEELPDEAAVDRDNLRLFGVKSNLTVPLLVGGTSNIGALGLNTTRV
                     ERDWPDALVKRLQLVAQVFANALARKHADEALRESEERLSLAADAAEAGLWAFDYSTG
                     VFWATGRARAIFGFSPDEVITPERLQASVHPDDWDLVREVIERPARADDPTHVEYRIW
                     PAEGGVRWISSTGRPRFTPAGDLDRLMGVVVDITERKRAEEALLASEARLASGAELAG
                     LAFYEVDFGAGAVYIDDRFRDLCGLPPDRERGLQPLEFWMDHLHPDDRRRVLDAREEL
                     HDGRLEQLFLEYRFVLPSQEQKWIQHIGRVAARDVDGHTVKSYGVLRDVTERKRAEQE
                     LHDLSRRLIRAQEEERALLARELHDDVSQRLAVLAIDVGRSELGAADRTQAATMQAIR
                     EGLMRLSEDVHSLAYQLHPSVLEELGLGEALRAECERRGRRSQLELSLDLGPLPGDLT
                     KDAALCLFRVAQEALNNVARHAQASLATVALRQMDGGLLLAVSDDGVGFDAEHPGKGM
                     RLGLASMRERVQLVNGTLDIETAPGRGATIVAWVPVQRGSE"
     gene            14141..14791
                     /locus_tag="VFZ86_14495"
     CDS             14141..14791
                     /locus_tag="VFZ86_14495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5643527.1"
                     /translation="MSASRRPRVLLADDHLMVAEALTSLLSPEFELVGVVEDGRQLVE
                     AAARLRPDVIVADITMPHLNGIDALTRLRADGDQVPVVFLTMHRDATFARRALEAGAS
                     GFVLKHSASVELIAALHAALEGKTYLTPQLAGEVLEAMRLGPEHAADPAASLTLRQRE
                     VLQLLAEGRSGKEIATSLSISIRTVEFHKYQMMETLGLHTNAELIHFAIKNGLVEF"
BASE COUNT         2400 a         5531 c         5339 g         2224 t
ORIGIN      
        1 gcgaagccgt gttgactggg ccgcgccccc tgggtcacga tcctcgcctc ccctcgtggc
       61 cgagcgcccg aacacgcccg gcgtcctgac gtcccccgca cagtctacgc cgtggcgaac
      121 gcgggctcac gcaatcgtcg actcggcccg ccgtatccga cagagtccgc ccgcgcgtgc
      181 aactcctgcg tcgggacgac cgacggccac gccgacatcc acggcgccaa cgagcgcgtg
      241 ctgcaggacc agtccgagaa ggccacgccg gccgaggccg acttcttcgg caggctggcg
      301 acgggtggcg cgcgatgagc gccgcggcct gatgcgccca ccggcaccgg ggaggtacac
      361 tcgcccgggt acgtccgtcg cgtcagcgtc tcacccagga ggaccgatga agtcccgccg
      421 ttccatcgcc gccggtcgcc ggcgccggcg ccctgcgcgc gggcgtcgca ctcgtctcgt
      481 ggtcttcatc gccgtggcgg gactcgcggc tgcggttccg ttcgcggcgt cggcctgcgg
      541 ctcggacgac tcgacagact cctcagtgca ggacgccgcc gcgctcggcg gccggatctg
      601 gaaggccgtc gagatcgcgg gcgtcacgac ggcgccggca gacggagccg acgccaccgc
      661 cgcgttcgag ggcggcgtcg tcagcggctc gggaggcgtc aatcgctaca ccgccgccta
      721 cacgacggag gagccggacg ggatcaccat cgcgcaggcg gccgcgacgc tcatggccgg
      781 cccggaggaa gcgatggcgc aggagcaagc gtacttcgcg gccctggccc aggcggcgac
      841 gttcagcgtc gacgaggggt ccctcacgct gatggacgac cagggcgcga cgctcgtgcg
      901 cttcgaggtc gcggcgccga gggcgctcac aggaacgacg tggcaggccc tggcgtacaa
      961 caacggcagg ggggctctgc aatcaccggc catcgacagc agcatcacgg ccgtgttcga
     1021 cgaggatggg aacctcggcg gcaacgcatc cgtcaaccag tactcgaccg cgtactcgac
     1081 gacgggcgag acgatgtcca tcgacgcgca gatcatcact accaagatgg cggggccgcc
     1141 ggagctgatg gagcaggagg cggcctacct ggcggcgctg ccacagaccg cgacctacac
     1201 ggtcgagggt gacgagctct ggctgcggga cgcggaaggc gcggcgctgg cccactacgt
     1261 agccgagtag agcgccgcat gtggcctggc ttgacgcggc cgcggcggcg tcagagtacc
     1321 atgaggccat gagcgagacg cccgtccccg ccttccacgt gatggccaag cccaccggcg
     1381 cgcgctgcaa cctgcgctgc gactactgct tcttcctcga gaaggaggag ctgtatccgg
     1441 gcagcgactt ccgcatgcgc gacgaggtca tggaggcgta catcgcgcag acggcgcagg
     1501 cgcagcgggt gccggccgtg acgctcgcct ggcagggcgg cgagcccacg ttgatggggc
     1561 tcgacttctt ccgccgggca cgcgcggccg aagagggccg cgtgcccgcc ggcatggccg
     1621 tcgagcgcac gctgcagacc aacggcgtcc tgctcgacga cgagtggtgc gccttcctcg
     1681 ccgagaacga ctacctcgtc gggctcagca tcgacgggcc gcgcgagctg cacgacgcct
     1741 accggcacga cgccggcggg cggccggtct tcgaccgcgt cgtcgcggcc gcccgccgcc
     1801 tgcagaagca cggcgccgag ttcaacgtgc tgtgcacgat caatgcggcc aacgccggcc
     1861 acccgctcga ggtctatcgc ttcttccgcg acgagctcgg cgcccgctac gtccagctca
     1921 tcccgatcgt cgaggtcgag acgccgccgg ccggcggccg gccgggcacg gtcagcgcgc
     1981 gcagcgtaca gcccggcgac tacggccgct tccttaacgc gatcttcgac gagtgggtgc
     2041 gccgcgacgt cggcgagatg ttcgtgcagt tcttcgacgg cgtgctcgcg gcctacgtgc
     2101 gcggcgtctc cagcctctgc atcctgcagc ccacctgcgg cgagggcgtg gcgctggagc
     2161 acaacggcga cctgtactcg tgcgaccact tcgtcgatcc gccgtacctg ctcggcaaca
     2221 tcatggagac gcccgtcggc gacctcgtgc gctcggagca gcagcgggcg ttcgggcggg
     2281 ccaagcagga gacgctgccc gccttctgcc gcgcctgcgc gtacctcttc gcctgcaacg
     2341 gcgagtgccc caagaaccgc atcctcgtga cgcccgaggg cgagcccggc ctcagctggc
     2401 tctgcgccgg gctcaaggcg ttcttcgccc acacggaccg gcgcatgcgc ctgatggcgg
     2461 acatcctcaa gcgcggcggc gaggctccgg aggtcatgga actcctcgcc gccgaggccc
     2521 gcggcaccgg tcgcaacgac ccctgcccct gcggcagcgg caagaagtac aaacgctgct
     2581 gcggcattgc cgcccgctga gcgcatccgc tagtctgctt tccgagcggc cgcccgccgc
     2641 gactcacgag cgccgaccga agaggagtgc gccatgccga acggcaagcc caacatcctc
     2701 gtcctctggg gcgacgacat cggcttctgg aacctcagct gcaacaaccg cggcatgatg
     2761 ggctaccaga cgcccaacat cgaccgcctg gctcacgagg gcgtcggctt caccgacttc
     2821 tacggccagc agagctgcac cgccggtcgc gccgcgttca tcaccgggca gaaccctctg
     2881 cgcaccgggc tcaccaaggt cggcctgccc ggctccgact tcggcatccg gccggaggat
     2941 ccgacgatcg ccgagctgct caaaccgctc ggctacgcca cggcgcagtt cggtaagaac
     3001 cacgtcggcg acctcgacga gcacctgccc accaaccacg gcttcgacga gttcttcggc
     3061 aatctctacc acctgaacgc cgaggaagag cccgagcacg aggactaccc caaggatccc
     3121 ggcttcaagg aacgcttcgg accgcgcggc gtcatccaca gctgggcgca gcccgacggc
     3181 acgcagaaga tcgaggacac cggaccgctc acgcgcaagc gcatggagac catcgacgac
     3241 gaggtcaccg cgcgcacgct ggagtacctg gacgaggtca cggccgcgga caagccgttc
     3301 ttcctctggt ggaacgcgac gcgcatgcac tttcgcacgc acgtcaagga ggagaaccgc
     3361 ggcgtcagcg gccaaggtgc gtacaacgac ggcatggtcg agcacgaccg ccacgtcggc
     3421 atgatcctcg acaagctcga cgagctcggc atcgccgacg acaccatcgt catgtactcc
     3481 accgacaacg gcccgcacta caattcctgg ccggacgcgg ccatcacgcc gttccgctcc
     3541 gagaagaaca gcaactggga gggcgcctac cgcgtgccgg cgttgctccg ctggccgggc
     3601 aagtggcagg ccggggtgac gctcaacggc atcgtcgccc accaggacat gctcaccacc
     3661 ttcctggccg ccgccgggga acccgcgatc gcggagaagt gccgggccgg ctacgacgcc
     3721 ggaggcacga ccttcaaggt cgtcctcgac ggcaaggaca tgaactccta cttcgcaggc
     3781 gagaccgacg agagcccgcg cgaccgcttc ttctacgtca acgacgacgc cgagctcgtc
     3841 gccctgaggg tcggcgactg gaagttcatc ttcaaggagc agcgcgcccg gcagctcgcc
     3901 atctggctgg acccgttcgt cgagctgcgc gcgcccaaga tcgtcaacct gcgccgcgac
     3961 cccttcgagc gcgcgctcga cgactccaac acctactacg actggatgat ggaccgcatc
     4021 tacatggtcg tccctgcgca ggcggccgtg gctgaggccg tcgccgagct cagggagttc
     4081 ccgccgcgcc agaagccctc gtcgttcaat ctcgaccggg tgctcgagaa gcttcaggag
     4141 gccgggggca gccagagctg atccgaatgc cgtacaccat gaaaatcggt ctgctgcgga
     4201 ctgattttca tgatagaatg ccgccatgat accccgcacg gaactgcgcg tgcgcatcga
     4261 cgccgcgctt gggcgcagtc ccatcgtcgt cctcaccggc cctcgtcagt gcggcaagac
     4321 gacgctggcg cgagagctgc tctcagccga ttccgagaac tacttcgacc ttgaggatcc
     4381 ggcgagcctc gctcgcctgg acgagcccat gacggcgctg cgcccactgt ctggtctggt
     4441 agtgatcgac gaggtccagc ggcttcctga cctctttccc gtgctgagag tactcgcgga
     4501 cagatcacca tcgccggcac ggttcctcat tctgggcagc gcctcgggtg cgcttcttcg
     4561 ccagacgtcg gagagcctgg ccggacgggc cgagcgggtc ctcatgggcg gattctctgt
     4621 ggacgaggtg ggcggcgcgg aggcggacga cctttggcgc aggggaggcc tcccgaggtc
     4681 ctttctcgcc gcgaccgagg ccgacagcgt ctcgtggcgc agccagttca tccaggctct
     4741 catggagcgt gacctcccgc agtggggcgt gcgcgtcccc gccgtcgcgc tgcgccgctt
     4801 ctggacgatc ctcgctcact accacggcca gatctggaat gcgacggatc cggctgcgtc
     4861 gatgggggtg gggacaaagg ccgtgcgcca ctacctggac ctgctcacgg atgcctacat
     4921 gatccgccag ctgcagccgt ggcatgccaa cctccgcaag cggcaggtca aggcgcccaa
     4981 ggtctacgtg cgcgacaccg gccttctgca ccagctcctc ggcatcacga ccgcggctga
     5041 gctctacacg catcccaaga gcggagcctc atgggagggg ttcgcgatcg agcaggtgct
     5101 ggccgggcag ccgcacgacg acgccttctt ctgggccacg caccagggag ccgagatcga
     5161 tctggtcctc cgccgcggcg agcgactgct gggcgtggag tgcaagcggg tcgacgcccc
     5221 ccgcctgacg gcgtcgatca ggatcgctct cgaggatctg ggactcgagc gcgtggtcgt
     5281 cgtgtatccg ggggagcgcc ggtatctcat cagcgacaac gtcgaggcgc tgcccctgca
     5341 cgaactcgcc ggcgcgccct cgttgttcgg cgcctgacac cggccgcgcg aggcggacgg
     5401 ccgcgacgcc tgcgacacgc gccgaggcgc gtcagccggc cgagcggccc gggagccgct
     5461 tgcgcacggc gcgagaggcg agcgccgtgc cttcgccgac ggccatgccg acgagcgacc
     5521 ccacgatgac gtcccccggg tagtgcaccc cggagtggac gcgcgagtac gcgaccacgg
     5581 tcgcgagccc gcgcaggggg gtgccgaggg ccgggatcgc ctcggcgacc ccggcggcga
     5641 acgcgaaccc ggacgccgag tggccggatg ggaacgaggt cgacgtgggc atcttcacgt
     5701 accgggcctc ctcgaaccgt acgacaccgc gctccggccg cgggcggtgg ccggcgaact
     5761 tcatcggcac gttgacgacg aacgagttga gccccacggc agccatcccg gtgacggcgg
     5821 cgcggcggcc cttcttgccg ccggcggcga acagcagcgc cgcgatgccc atgtacagct
     5881 tggcgtggtt ggcggtgttc gagagacgcc gcagcggcgc atcgatgagt ggcgtgggcg
     5941 tggcctcgat ggcctgatac acggcggtgt cgagcgccac gagctcgctg aacactcccc
     6001 gcgcgacggc ttgggggctc cgatcgtccg ccgggagact ctggttgtcc gctgtgtcgg
     6061 ccacgtgcag atgctaccat tcgtgaccga ctggtaaagc aggggggtcg gggtggcgac
     6121 gaaagccgag cagaccgagg cggcgcccgt caagaggagc cggctggacg cggtggtccc
     6181 gggcgtcggg atgctgcgcg cgtacaagcc ggcgtggctg cgcttcgacc tcgtcgccgg
     6241 catcgtcctc gccgcgatcc tcgtgccgca ggggctggcg tacgccgagc tggccggcgt
     6301 ccctcccgtc accggcctct acacgacggt cgtgtgcctc atcgtgtacg cgctcttcgg
     6361 gccgtcccgg gtgcttgtgc tcggcccgga ctcgtctgtc tccccgctca tcttcgccgc
     6421 catcgtcccg ctcatggtcg ccgacgatcc cgcgtcggcg atcgccctcg ccggcatgct
     6481 ggcgctcatg gtgggcctca tcgagatcgg ccttggactc ggcaagctgg ggttcatcgc
     6541 cgacctgctc tccaaggagg tccaggtcgg ctacatgaac gggctggcca tcacgatcat
     6601 cgtcggccag ctgcccaagc tcttcggctt ctccaccgac gcggactcct tcgtcgacga
     6661 cgtcacggcc ttcttcacga acctcgacca gaccaacacg acgacgctcc tcgtcggcct
     6721 cggcacgctc gccctcctgt tcggccttcc ccgcatctcg aagaagctcc cggcgatcct
     6781 cgtcgccgtg gtcgtagcca cggtgatcac ggccgcgctc ggcctcgcgg agaagggcgt
     6841 cgacaccgtg gggacgctgc cgcagggctt gccggtcccc tcgttcccgt ggaccgggtg
     6901 gagcgacctc gcgaccctgg ccgccgccgc ggtcggcatc gtgctggtct cgctgacgga
     6961 caccatcgcc acctcgtcga gcttcgctgc ccggcgcggc gacgaggtgg acgccgaccg
     7021 cgagatgatc gcagtgggcg cctccaacgt cggcgccggc ctgttccagg gcttcgcgat
     7081 ctcggtgagc agctcgcgca cggccgtggc cgaccgctcc ggcgccaagt cgcaggtcgc
     7141 cggcctggtc ggggccggcc tcgtggcgct catgctcctc gccgtccccg gtctgctcaa
     7201 gaacctcccc cagacggcgc tggccgccgt cgtgatcatg gcagccgtct ctctggcgga
     7261 tatcgccgtc ctgcgccgct tcgcgcacgt gcgcaagacg gcactgcttc tctccctggt
     7321 ggccacagcc ggcgtggtct tcttcggcgt gctggagggc atcctcatcg ccgccatcct
     7381 ctcgatactc ctgttcttcc gccggagctg gtggccgccg ggcgccgtcc tcgggtacgt
     7441 cgccgagctc aagggctacc acgacgtcga gcggtacccc acggccgagc aggagccgga
     7501 catcgtgatc ttccgctggg aggcgccgct gttcttcgcc aatgccggca tcttccggca
     7561 gcgcgtgcgc aagctggtgg tggagcgcaa gccgctctgg atcgtgctcc agtgcgaggc
     7621 gatcaccgac atcgacgtca cggccgccga cgtgctgagg cagctcgatg aggagctcaa
     7681 cgcgaaaggc atccacttcg cgttcgtcga gttgcgcgag cggctgcagg accggctcag
     7741 gctgtatggg ctctggcaga cgctgaaccg ggagcacgtg ttcaacaaga tgaagccggc
     7801 gatccgcgcg ctctcggagc acggacccta cggcgaggac ggcccccacg ggcccgcggg
     7861 cttgcggcac cttgagcccc cggacgagcc cgagagctga gccggcggcc gatcagcgag
     7921 cagcgcgtcg cggcacaggg cccgccggcc cacccgctcc ggtcatccgt cgatgccgac
     7981 cggccgcccg agcaccacgc gccacaggcc cagcagaccc ctgacccccg tcgccctggc
     8041 ggccggcgag tagccgatcg ccctgagcgg cagccggagg cgcagcacgc ccggccgcga
     8101 ccggaacacg agcggcggct ccatgtcgag cgcctcgccg tcaagaccga cggcgatcgg
     8161 cgagcccgag tccacttcga aggtgggcgt cgcccaggtg agatacccat cgtagagcgc
     8221 cggatgggcc gtcgtcagcg ccgcgatggc gcgcgtcgcc gagacggcgt cggggacgac
     8281 cagcgagaac acaccgaggg tacccgtgtc gaggtgcggc cgcgaggtga tgcctccggt
     8341 ggtggtcccg tacgggccgt tggagacctg gatgacgtgc gccccgtcgt gctcccggcc
     8401 gtccggggtg ctgaagcgca ggtcgaacgg cttggacccg gggcccagca tctcaggcag
     8461 cgccgccagg gtcgtctccc gcttggcgtc gcggtactcc ggagactgca cgatggtcgc
     8521 gtacagaccg agcgacacgt tgttgacgaa gatgcgcccg ttgacgtcgg cgaggtcgat
     8581 gacgcgctcg cgcgcctcgt cgtaggcatc gagcgcgccg accacgtcct cgcggtccag
     8641 gccgatgtcg agcgcgaagt ggttgcgcgt gccggccggg atgacgacca tcggcacgcc
     8701 gcgctcggcg gcgatgctgg cgaccagcgc ctgcgagccg tcgccgccgg ccatgccgat
     8761 cacgtcggcg ccgccgtcga tggcgtcgtg cgcgagctgg agcaggtcgt cgccgcgctg
     8821 cagcacgacc ggctcgatcc ctcgcgcgcg acactcgtcc acgaggtgga acttctcggc
     8881 cttcccgccc ccggacttga ggttcatgat gaggacgccg cgccgcgcgg ccggcgccgg
     8941 cttccccggc gtctccgtct gcttgagtga gcgcacgtcg cgcgtcagcg cgtagcgcga
     9001 cagcagcagg gcgacaccca ggagcgccag ccgcaccagc agggagagca ggtgcttctc
     9061 gctgccggcg atctggatga ccaccacggc gacggccgcc acgacggcga cgacgatcca
     9121 ggtcccgcgt accgcgcctt ttctcgtgag cgcgttccac gccgcgacga gcacgacggc
     9181 cagcaggacg acctgcacca ccacctcgag cggccggtcg aggacgacga gcaccgtggc
     9241 gacgaagact ccaaggaaca gcgcgatcgc agctgcggcg gcgagccgtc gccctggcgc
     9301 aggtccctgt gacgcggtgt gttcggtcac gacgcctccc ctcactcgtg tccggcaggt
     9361 ccgctctgct ccccgtctgc cgccggcgac atcgtgccac gagaacgttc ccgctgccag
     9421 gccgcgagcg cgtgggcgtg cgggcgggtg gcgacagcga tctcgcggaa ccacccgtag
     9481 agctcgtcgt agagggcccg atgtcccgga tccggctcca cacggaccgt cgcctgtggc
     9541 cacgtcgtct gcagggtcgc cgcaccgacc gacaccgccg cgagcagcgc cgcgccgaca
     9601 cccgcctggc tcggaccctg gcgcacgtcc tgcgccaggc cggtggcgtc actgacgatc
     9661 ctcggccaca gggcgccggc gacgccgccg ccggcgctgc gcaacgcgac gatggtggcg
     9721 ccggccgcct ccatcgtctc gaggttctga cgcacggcga acgcggcagc ctcgaggagc
     9781 gcacggaaga ggtggcctcg tccgtgcgcg gcggtcaggc cgacgatggc gccgcgcagg
     9841 tcggggtcga acagcggcgt gcgctcgccg gcgaagtagg gcagcgcgag gaggccgccc
     9901 gccccgggcc cggcggcggc ggcctcagcg tacagccgct cgtaggccgg gccgccggca
     9961 atgtccctga gccaggccgt gagcgcgccg ctcgaggcca cgcccgcggc gatgttgagc
    10021 gacctcggcg cgaacccgac ggtgctccag agccgggggt cggggcgggc cgggctgtcc
    10081 acctcgatga ggaacatgct ggtcccgtag acgagtagcc cctggccggg gccgcgcagc
    10141 ccggacgcag ccacctcgca ccaggagtcg atgcttccgg cggccaccgg gacacccgcg
    10201 gcgatcccga tcgcggcggc ggcctccggg ctgacgccgc cgcacacctc gtgcggccac
    10261 acgagccggg gcagcgccgg cccaggagcc gcggcgggga cccactcgtc gatccagtcg
    10321 ttctcatgga cgtcgtagag cggcgcccag tggctggcgc tgtggtggtc gaggacgtac
    10381 tcgccggtga gacgcgcgac gaggtaggaa ctcgcgccgt acacgcgccg tgtcgccgac
    10441 catacctccg gctcccgctc agcgagccag cggagcttcg gccccaccga ctggctcgag
    10501 aggcgggagc cgcagcgagc caggacggcg tcggagccga gttctgcggt caaccgctcg
    10561 gcctgggcga cggcccgcga gtcgataccg tacaggatcg cgggccgcag cggccgcccc
    10621 cgtgcatccg ccggcaagac gcagggaccg aggccgctca ggccgacggc gcgcacgcac
    10681 gcgccacggc ctttcagcac gcggcgggac acgtcgagta cgctgcccca ccagtcgcgc
    10741 tcggcgtcct gctcggccca gcccgggtgt ggtcgcaaga tctcgtgctc ggtcctggcg
    10801 tcggccacga ccccgccgcc ggcatcgacg agcaccgcct tcgtgctgga cgatccggcg
    10861 tcgaggccga gaaagaggtc ctcagtcaac ctgtccgcct gttcaagtgg ttccttcttg
    10921 tcgcgctgtc gagtgaacct actccacagc cgaaggcacg ccaatgccgc ctgctcggag
    10981 tcgtcgtggc gccggacgac ccggtgagct gagaaggccg ccttcgccga gtgacgcgcg
    11041 acgccggcga gggcggcctt cgtggtgagc agcgacgcac cggcaggggg gcgccggccg
    11101 gcgctgctac tcctccgacc ccaccgcgac gaggccgatc atgatgaaca cgtacatggt
    11161 gatcaggagg atcgaccaga cggggtagat cggcaaccaa aggagttcgg agatgatggc
    11221 gacgcccgcg gcgaccacgc cgacccagcg tccccaggcc ttcccgttca tcacggccag
    11281 accggcaagg aggaggatca cgccgacgat gaggtaccac caggcgagtg cgctgatgtc
    11341 gatgaagtag taggcgttga agccgcgcac cagccactcg tcgttgaaca gaccgatgaa
    11401 gccggagatc gccttgaagc cgccgacgaa caccatcaat atcgcgccgg ccgccgccca
    11461 cccctgccac ttgccgtcca tacgccctcc tgtccgccgc ccgcgcgcac gcctctgcgc
    11521 gcgggcacag tcccaacatg tcgcgctgag actagtgctg atccggaggg ctgtgtactc
    11581 gggaaactac gggcctggtc gcgggaaact acgggccggc tagcctgccc tgaggcgagc
    11641 tatggcgggc agaaggtcgg tcgcgatcgc gcgcttgagc acgacggcat gggccccggc
    11701 gtccagggcg gcttgccgca cgctctgctc gtcgtggacg ctgagcacga tcaccttgac
    11761 ctcgggacag cgtgcgcgga gcgcccgcaa ccagtccagg ccgctgtgcc gagccagaga
    11821 gaggtccacg accgccacct cgggctgcag ccttcgcgcc ccttcgagca gcgacgtctc
    11881 gtcggcgacc atgaccaccg ttccgaacgc cgactcgagc agcccgcgca cgccctcgca
    11941 gagcgcctga tgaccgtccg cgagcaggac gcagctgaga tgcttctcgg gcatggggcg
    12001 atccctctcc tcggccgggg tatggcgcgc atcgcgcggg tcccggtgtc ggggtgaaga
    12061 ctactgggga cacgcctcgc tgtgacctcg ggaaactacg gggcgcgtcg ctgggggatc
    12121 gcgggccgat gatgtacctt ggtcgcatgg ccgacgtcga tgcgactgag cgctccccgg
    12181 aggacgaagg cggccgtctc gagttcgaga cgctgctctt cgacctttcc tcgagtttca
    12241 tcaacttgcc ccctgagcaa gtggaccggg agatcgagga cgtccagcgg cgcgtgtgcg
    12301 aggtcctggc cgtcgacctc tcggcgctgt gggaggtcac ggctgcaggc ggtgccccac
    12361 tcaagctcac ccacttctac agctcgcagg aggacctcct gcctcctatg cgagggatga
    12421 gcgctcagga gtacttcccc tggctccacc gggagatgct ggccgggcgc agcgtcgccg
    12481 tctcctcgct cgaggagctt cccgacgagg ccgcagtcga ccgcgacaac ctccgcctgt
    12541 tcggggtcaa gtcgaatctg accgttccgc tgctggtggg gggcacgtcg aacatcgggg
    12601 cactgggact caacaccact cgggtggagc gcgactggcc ggacgcgctg gtgaagcggc
    12661 tgcagctggt cgcgcaggtc ttcgccaatg cgctggcacg aaagcacgcc gacgaagccc
    12721 tgcgagagag cgaggaacgc ctgagcctgg cggcggacgc cgcggaagcg ggcctgtggg
    12781 ccttcgacta ctcgacaggg gtcttctggg ccacagggag ggcccgtgcc atcttcgggt
    12841 tctcgccgga cgaggtcatc acccctgaac gtctgcaggc ttcggtccat cctgatgact
    12901 gggacctcgt gcgcgaagtc atcgagcggc cggcgcgcgc ggacgatcca acgcacgtgg
    12961 agtaccgcat ctggcccgct gagggcggcg tgcgctggat ctcatccaca ggacgcccgc
    13021 ggttcacccc cgccggcgac ctggatcggc tgatgggcgt ggtcgtcgac atcaccgagc
    13081 gcaagcgggc cgaggaggcg ctcctggcca gtgaggcgcg gctggcctcc ggagccgagc
    13141 tcgccggcct cgccttctac gaggtggact tcggcgccgg cgccgtctac atcgacgacc
    13201 ggttccgtga tctctgcggc ctgccccccg accgtgagcg ggggctccag cctctggagt
    13261 tctggatgga ccacctgcat cctgacgacc gccggcgggt gctggacgca cgcgaggaac
    13321 tgcacgacgg gcgattggag cagctgttcc tcgagtaccg cttcgtcctc ccgagccagg
    13381 agcagaagtg gatccagcac atcggccgcg tcgccgcgcg cgacgtcgac gggcacacgg
    13441 tcaagtcgta cggcgtgctc cgcgacgtca cggagcgcaa gcgagccgag caggaactgc
    13501 acgacctgag ccgccggctg atccgggcgc aggaggagga gcgcgcgctg ctcgcccgcg
    13561 agctgcacga cgacgtgagc cagcggctcg ccgtgctggc gatcgatgtc ggccgcagcg
    13621 agcttggggc cgcggacagg acgcaggcgg cgacgatgca ggcgatccgc gaggggctca
    13681 tgcgcctcag cgaggacgtc cactccctgg cctatcagct gcacccatcg gtgctggagg
    13741 agctcggcct gggcgaggcg ctgcgggcgg agtgcgagcg gcgtggtcgg cggagccagc
    13801 tggaactctc cttggacctc ggtccgctgc ccggcgactt gacgaaggac gcggcgctgt
    13861 gcctgttccg tgtggcccag gaagcgctga acaacgtcgc tcgccacgcg caggcgagcc
    13921 tcgcgaccgt cgcgcttcgc cagatggacg gcggcctgct cctcgccgtg tctgacgacg
    13981 gcgtcggatt cgatgcggag catcccggaa aggggatgcg gctcggattg gcgagcatgc
    14041 gcgaacgggt gcagttggtc aacgggacgc tcgacatcga gaccgccccg ggccgcggcg
    14101 cgacgatcgt cgcgtgggtg cccgtgcagc gaggctccga atgagcgcat cacgccgtcc
    14161 gcgcgtgctt ctcgccgacg accacctgat ggtcgccgag gcgctgacga gcctgctcag
    14221 cccggagttc gagctcgtgg gtgtggtcga agacggccgc cagctggtcg aggcggcggc
    14281 gcggctgcga ccggacgtga tcgtcgccga catcaccatg cctcacctca acggcatcga
    14341 cgccctgacc cggctgaggg ccgacggcga tcaggtgccc gtcgtgttcc tcacgatgca
    14401 ccgggacgcg accttcgcgc ggcgcgcgct cgaggccggc gcttccgggt tcgtcctgaa
    14461 gcactcggcc tccgtcgagt tgatcgccgc cctccatgcc gcactcgagg gcaagaccta
    14521 tctcaccccc cagctcgcgg gcgaggtcct ggaggccatg aggctggggc ctgagcacgc
    14581 cgcagacccg gccgcctccc tgacgctccg gcagcgcgag gtccttcagc tcctggcaga
    14641 gggccgctcg gggaaggaga tcgcgaccag cctgtcgatc tcgatacgga ccgtcgagtt
    14701 ccacaagtac cagatgatgg agacgctggg cctgcacacc aacgccgagt tgatccactt
    14761 cgcgatcaag aacggcctcg tcgagttctg acgttcgccg gcggccccgt agttcccgag
    14821 cggcacaccc cgtagtttcc cgaggtcaaa gtcctccgca tcgcgagtag cctctcctcg
    14881 acgaccttgc cggtgggcga cctggaccgt cgatcaccag agcgggagac gacatcatga
    14941 ccactcatgc cccatcagcc tcccctcccg gccaacggat gctcgaggac gagcggatat
    15001 cccctcgcga agcgcgccgc agcgaagccg aggtattgcg gcgacgctgg gggtggcgcg
    15061 cctgcgaccg atgcggcgac atcatgatgc tcggcgaagc gatgctccac ctgcgcttcg
    15121 gcgagcgcgc cgaagaggtg tgcctgggtt gcgccggcgc cccgagcgac tcgtctgggg
    15181 gtccgtccgc acccgggtcc gctcgatccg atgtgatgta gcgagcagtg ccgcgtcctg
    15241 ccacgaccgt ttggcagctc tgcatccatc ccccttcaag ccggcaacga ccgcgacgat
    15301 ctgggaccac gggtctccat gctcgtgacc cgtccgtgga ggtacagctg atcgaggcct
    15361 gcagccgggg cgcggcacgc gaagacgagc ggcgttgacg aaaggtgcgc gcggcgtgcc
    15421 acagccagga ccaagcgtat cgccggtgga gacactcggc gtgaaggagg caggatgaag
    15481 gtcatcatcg tggg
//
LOCUS       DASUMX010000308         6872 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_549345, whole genome shotgun sequence.
ACCESSION   DASUMX010000308 DASUMX010000000
VERSION     DASUMX010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 6872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6872
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549345"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="VFZ86_14500"
     CDS             <1..308
                     /locus_tag="VFZ86_14500"
                     /inference="COORDINATES: protein motif:HMM:NF036707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEX5643528.1"
                     /translation="NVGTFVGGFGWSVNEHQPPEIPAAPAIMVHFIREIDGGVEWRTR
                     FWMGYTILNGHPVPLLPPGIRVPEPVVFGLANHNIHEYTRLKSLLPLVYEEFGGTFA"
     gene            complement(373..1323)
                     /locus_tag="VFZ86_14505"
     CDS             complement(373..1323)
                     /locus_tag="VFZ86_14505"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX5643529.1"
                     /translation="MSAPPSATARGLTISQLEARSGVRRSVVYYYVHLGLLPPAQKAS
                     PTRALYTDRHLTLLAAIAELKHEGLSLEQIRERLAERIDTNIAGEVDLVADEERRRRD
                     VIVEEAARQFAAKGYRLTRIADICEALGIAPRTLYDHFPTKQALFVACYHVYADWLAA
                     DVEPHAEKERDPAARLIWRMRASAAIQAFGPDLQALAQAASVHDDGELQEMTQETYRV
                     ITERAEADITALRDPETAAPCSEELAAMGLQGAFERMLMRVAWDDEYALRDAMRTQLV
                     LYLALEAVLDGRLDVSGRLAQVEPLLDEVSRRPLPAPPPA"
     gene            1495..2277
                     /locus_tag="VFZ86_14510"
     CDS             1495..2277
                     /locus_tag="VFZ86_14510"
                     /inference="COORDINATES: protein motif:HMM:NF037296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643530.1"
                     /translation="MLKSIVMMDLPIDDIAEMERWYYEAHSSEISRRYGPFLQRHESY
                     VALHAPEDARELGFYNWRVTETFWREMPEPGAKGAFCFTPAPVWNPVATCTVKAQPDN
                     DFLGWDGFSTQGASIRWYILFRYPLGVRLSEGEDWFLNVHAPEVCKQPGLTRFFSYKC
                     EQAAIPLPGMWHPDATPPQDMMMVQWDRVVELWYPSFSAWRRAVIEAPPEYTAPEWAT
                     DDFFKDGYAAYPFFKPGVDFASTFLLERPADEFLRDTRYYRP"
     gene            2303..3136
                     /locus_tag="VFZ86_14515"
     CDS             2303..3136
                     /locus_tag="VFZ86_14515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005035957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEX5643531.1"
                     /translation="MAVRMLTPEEEAKPYAKYYHRSLTPPDPDLIAQILPDVQIDPAL
                     ALPPERISDLLDPGYHEVETGWCVMPNGSGYLAVHNRMPGVTVEMLDWWFWWHSMADM
                     RYGLWYPPGHYGISISKKNRAKLSDPSVPAKEKIYGRTDHVVEDIGTGAEDIYISFCA
                     PEQMGFEMDRFHAPNVAAVYGGFGFDQAQGAKPSDIRAPAIMCHFIREIDGGIEFRSR
                     FWMGYKLIDGKPVLAIPPGESVPAEVPYGLAEHNVREYCNLKALLPEIWAEFKDADPA
                     L"
     gene            3165..3959
                     /locus_tag="VFZ86_14520"
     CDS             3165..3959
                     /locus_tag="VFZ86_14520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005031891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EthD domain-containing protein"
                     /protein_id="HEX5643532.1"
                     /translation="MPIKLMAASRRRPGLTRAEYFRYIEHYHGTVARLERFKIEKYIQ
                     NHVIDGAFGVLSDADGHKNKVADRDAVVELHFDSFRDMLATLEPEGVTTSRANQDGKF
                     FADEPTNIIVMAEETELPVAKPKPDFNPGLGEPGNGALKVMQFIMRRPDVFPQDFHRL
                     WRQAHDEALAASPYANDMFRKIVVSKRSRVNDNDAAARAHFKMVDPPVYDLVVSFILD
                     SMEQVGAFRQYFDAVTASALEFADWSESFFLYVRQVRIIDDAPPKE"
     gene            3965..4873
                     /locus_tag="VFZ86_14525"
     CDS             3965..4873
                     /locus_tag="VFZ86_14525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005031893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEX5643533.1"
                     /translation="MPDMTKPEWAAQKTYDENRAFSKTKATRPSLTPDEEKRPFAKYY
                     YDEIPQPDPVHYATMDEPMDPADAFYPEDINRLLDLPALEAGVEIGWCNLPNGAGYIA
                     NRIHYPEFTADMVDWWFAWHPLEDLRYRIWYPPQHAGIMLSPIDRARILDDSVPDREK
                     NWGVVHNVTENCDNGYENIDIAFRSPADMGFDLEKFGEVAATMAGGMGWSVSVDKDDE
                     SITAPAIMCHMLYDAPEGGLIHRTRFWMGYRFNEEAKPELCLPPGVSVPVEAVQGLAR
                     HNVKEYTRWREFLPRIYAELGPGMYC"
     gene            complement(4951..5892)
                     /locus_tag="VFZ86_14530"
     CDS             complement(4951..5892)
                     /locus_tag="VFZ86_14530"
                     /inference="COORDINATES: protein motif:HMM:NF018184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX5643534.1"
                     /translation="MDPLQARDKTPDTGAQRLMPIGIFSRMTGLSHRALRLYADRGLL
                     PPAHVDESTGYRYYDVHSIRAAEMIRLLRRLGVPLGEMRLYIDAAASDRLEETLTQQK
                     QRLEQERSRLDAALRLLGRIDELDGMFREAPAVELLDLPAERCLRWSGAMARAEFHES
                     YIELAGALAARAADLGLRPAGREVVLLRDPTDKGLAGGDETVLNYDLCLPVDGDAGED
                     GRSDLMELDGGRFARSVFTGLYEDGYRFAYARQLEWLADSGRKLRGLLRMRFIRDERD
                     TADPADFATELVWPVTEPVSEGARERRPDLTHGWLRP"
     gene            6005..>6872
                     /locus_tag="VFZ86_14535"
     CDS             6005..>6872
                     /locus_tag="VFZ86_14535"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX5643535.1"
                     /translation="MNEIIDGLAALVGRDNVAADPASLEEYAQDESFVPRLTPWAVVR
                     PGSAEEVQQVVAWANQTATPLVPVSSGGPHFHGDTVPSVPGAVMLDLRRMDKILRVDR
                     RNRMAVIEPGVTYPQLQAALAEQGMRVTPPLCPRANKSVVASLLERQPTLIPRYNFHL
                     PEPLRSCGVVWGNGDVMFTGEAGTGPLSLEAQWQGGLVQADPKGPGQTDFFRLLTGAQ
                     GTMAVVTWAAVKCELVPTAHEYLFAAAAELDELVDFVYRLDRIRLGDEVLILNDAYLA
                     RLLKAAGAEVAPE"
BASE COUNT         1119 a         2406 c         2306 g         1041 t
ORIGIN      
        1 ccaacgtcgg caccttcgtg ggcggtttcg ggtggagcgt caacgagcac cagcctccgg
       61 agatcccggc cgcgccggcg atcatggtcc acttcatccg ggagatcgac ggcggcgtgg
      121 agtggcgcac gcggttctgg atgggctaca cgatcctgaa cggccacccc gtccccctcc
      181 tgccccccgg catccgcgtg ccggagccgg tcgtgttcgg gctcgccaac cacaacatcc
      241 acgagtacac gcggctcaag tcgctgttgc cgctggtgta cgaggagttc ggcggcacgt
      301 tcgcctgacg accgactgag ctggagacgc gcgggcggcg gggatcccca gggaccccgc
      361 cgtccgtgcg tctcaggccg gcggcggggc gggcagcggc cgccggctca cctcgtccag
      421 cagcggctcg acctgcgcca gccggccgct gacgtccagc cggccgtcga gcaccgcctc
      481 gagggcgagg tacagcacga gctgcgtccg catcgcgtcg cgcagagcgt actcgtcgtc
      541 ccacgcgacg cgcatgagca tgcgctcgaa cgcgccctgc aggcccatgg ccgcgagctc
      601 ttccgagcag ggcgccgccg tctccgggtc gcggagggcg gtgatgtcgg cctcggcgcg
      661 ctcggtgatg acgcggtacg tctcctgggt catctcctgg agctccccgt cgtcgtgcac
      721 ggaggcggcc tgcgccagcg cctgaaggtc cgggccgaag gcctggatgg ccgcgctggc
      781 gcgcatgcgc cagatcagcc gcgccgccgg gtcgcgttcc ttctccgcat gcggctccac
      841 gtccgcagcc agccagtccg catagacgtg gtagcacgcg acgaagagtg cctgcttggt
      901 cgggaagtgg tcgtacagcg tgcgaggagc gatgccgagc gcctcgcaga tgtctgcgat
      961 gcgcgtgagc cggtagccct tggcggcgaa ctgccgcgcc gcctcctcca cgatcacgtc
     1021 ccggcggcgg cgctcctcat ccgcgaccag gtcgacctcg ccggcgatgt tcgtgtcgat
     1081 gcgctcggcg agccgctccc ggatctgctc caggctgagt ccctcgtgct tgagttcggc
     1141 gatagccgcg agaagcgtca ggtggcgatc cgtatagagc gcccgtgtgg ggctcgcctt
     1201 ctgcgccggc ggcagcaggc cgaggtgtac gtagtagtag acgacgcttc tccgcacacc
     1261 gctgcgcgcc tcgagctgcg agatcgtcag gccgcgcgcc gtcgcggatg gtggcgcgct
     1321 caagtgcgaa gttccagcgt tgtcgaaggc tctgggatga cgattgctct cacgatgtga
     1381 ccctacgtca ttgacacctg acggctgcaa ctctagtgta cgtaatcaga ttgtcaacta
     1441 ctctagtaga cggcactcgc ggtgtcggtc gacgcacacg gcagaggggg atccatgctg
     1501 aagagcatcg tgatgatgga cctgcccatc gatgacatcg ccgagatgga gcgctggtac
     1561 tacgaggccc actcttcgga gatctcgcgg cggtacggtc ccttcctgca gcgccacgag
     1621 agctacgtgg cgctccacgc gcccgaggac gcgcgcgagc tcggcttcta caactggcgc
     1681 gtgacggaga ccttctggag ggagatgccg gagcccggag ccaagggggc cttctgcttc
     1741 actcctgcgc cggtctggaa cccggtcgcc acctgcacgg tcaaggccca gccggacaac
     1801 gacttcctcg gctgggacgg cttctccacc cagggcgcca gcatccgctg gtacatcctg
     1861 ttccgatatc cgctcggggt ccggctcagc gagggggagg actggttcct caacgtgcat
     1921 gccccggaag tgtgcaagca gccggggctg acgcgctttt tcagctacaa gtgcgagcag
     1981 gcggccatcc cgttgcccgg catgtggcac cccgacgcca cgccgccaca agacatgatg
     2041 atggtccagt gggaccgcgt ggtcgagctc tggtacccga gcttcagcgc ctggcgcagg
     2101 gctgtgatcg aggcgcctcc ggagtacacg gcgcccgagt gggctaccga cgacttcttc
     2161 aaggacggct acgccgccta tccattcttc aagcccggcg tggacttcgc gagcaccttc
     2221 ctgctcgagc gtcccgccga cgagttcctg cgtgacacgc gctattaccg cccgtgaacg
     2281 ctctgccgag gaggacagcc tgatggcagt acgcatgctc acgcccgagg aagaggcgaa
     2341 gccctacgcc aagtactacc accgctcgct cacgccgcct gaccccgact tgatcgcgca
     2401 gatcctgccc gacgtccaga tcgatccggc cctggcgctg ccgccggagc ggatcagcga
     2461 cctgctcgac ccgggctacc acgaggtgga gaccggctgg tgcgtcatgc ccaacggcag
     2521 cgggtacctc gccgtgcaca accggatgcc gggcgtgacc gtcgagatgc tcgactggtg
     2581 gttctggtgg cactcgatgg ccgatatgcg gtacggcttg tggtatccac cgggccatta
     2641 cggcatctcc atcagcaaga agaaccgagc gaagctgagc gatccgagcg tgcctgccaa
     2701 ggagaagatc tacgggcgca ccgaccacgt ggtcgaggac atcggcacgg gcgcggagga
     2761 catctacatc agcttctgcg caccggagca gatgggcttt gagatggacc gcttccacgc
     2821 cccgaacgtc gcggccgtgt acggcggctt cggcttcgac caggcgcagg gcgccaagcc
     2881 cagcgacatc cgggcgcccg ccatcatgtg ccacttcatc cgcgagatcg acggcggcat
     2941 cgagttccgc tcgcgcttct ggatgggcta caagctcatc gacggcaagc cggtgctcgc
     3001 tatcccgccg ggagagtctg tgccggccga ggtgccctac ggcctcgccg agcacaacgt
     3061 gcgcgagtac tgcaatctca aggcgctcct gcccgagatc tgggctgagt tcaaggatgc
     3121 cgacccggcg ctctgagcgc cgcaccggcc acggaggcaa gtccatgccc atcaagctca
     3181 tggcggcgtc acgccgccgg cccggactca ccagggccga gtacttccgc tacatcgagc
     3241 actaccacgg cacggtggcg cgcctcgagc gcttcaagat cgagaagtac atccagaacc
     3301 acgtgatcga cggtgcgttc ggcgtgctct cggacgccga cggccataag aacaaggtcg
     3361 ccgacaggga cgccgtcgtc gagctgcact tcgacagctt ccgcgacatg ctggcgacgc
     3421 tcgagcccga gggtgtcacc acttcacgcg ccaaccagga cggcaagttc ttcgccgacg
     3481 agccgaccaa catcatcgtc atggccgagg agaccgagct ccccgtcgcc aagcccaagc
     3541 cggacttcaa cccggggctc ggcgagccgg gcaacggcgc gctcaaggtg atgcagttca
     3601 tcatgcgccg cccggacgtc ttcccccagg acttccaccg gctgtggcgc caggcccacg
     3661 atgaggccct ggcggcgagc ccgtacgcca acgacatgtt ccgcaagatc gtcgtgagca
     3721 agcgctcgcg cgtgaacgac aacgacgcgg ccgcgcgcgc ccacttcaag atggtcgacc
     3781 cgccggtgta cgacctcgtg gtctcgttca tcctcgacag catggagcag gtcggcgcct
     3841 tcaggcagta cttcgacgcc gtcacggcga gcgccctgga gttcgccgac tggtcggagt
     3901 cgttcttcct ctacgtgagg caagtgcgaa tcatcgacga cgcgcccccg aaggagtgac
     3961 caccatgccg gacatgacca agcccgagtg ggctgcgcag aagacctacg acgagaaccg
     4021 ggcgttcagc aagaccaagg ccacccgtcc ctcgctcacg cctgacgagg agaagcgccc
     4081 gttcgccaag tactactacg acgagatccc gcagccggat cccgtgcact acgcgaccat
     4141 ggacgagccg atggacccgg ccgacgcctt ctacccggag gacatcaacc gtctgctcga
     4201 cctacccgct ctcgaggccg gcgtggagat cggctggtgc aacctgccca acggggccgg
     4261 ctacatcgcc aaccgcatcc attacccgga gttcacggcc gacatggtcg actggtggtt
     4321 cgcgtggcac ccgctcgagg acctgcgcta ccgcatctgg tacccgccgc agcacgccgg
     4381 gatcatgctc agcccgattg accgggcgcg catcctcgac gactccgtcc ccgatcgcga
     4441 gaagaactgg ggcgtcgtcc acaacgtgac ggagaactgc gacaacggct acgagaacat
     4501 cgatatcgcc ttccgttcgc cggccgacat gggtttcgac ctggagaagt tcggcgaggt
     4561 ggccgcgaca atggcgggag gcatgggctg gtccgtctcg gtcgacaaag acgatgagtc
     4621 gatcaccgcg ccggcgatca tgtgccacat gctctacgac gctccggaag gcggcctcat
     4681 ccaccgcacg cgcttctgga tgggctaccg cttcaacgaa gaggccaagc ccgagctgtg
     4741 cctgccgccg ggcgtgagcg tgccggtgga ggccgtccag ggcctggcgc gccacaacgt
     4801 caaggagtac acgcgctggc gcgagttcct gccgcggatc tatgcggagc tcggcccggg
     4861 catgtactgc tgagggccgg cgccctccga cacgactgag ctgacgccag acgggcgccg
     4921 ggaggacgag gatcaccccg gcgcccggcc ttacggccgc agccagccgt gcgtgaggtc
     4981 cggccggcgc tcccgcgcgc cttcggacac cggctcggtc accggccaga cgagctcggt
     5041 ggcgaagtcg gccggatccg ccgtgtctcg ctcgtcacgg atgaaacgca tgcgcagcag
     5101 gccgcgcagc ttgcggccgc tgtccgccag ccattcgagc tgtcgcgcgt aggcgaagcg
     5161 gtagccgtcc tcgtagaggc cggtgaacac gctgcgcgcg aagcggccgc cgtcgagctc
     5221 catcaggtcg gatcgcccat cctcgcctgc atccccgtcc acgggcagac acaggtcgta
     5281 gttcaggacc gtctcgtcgc cgccggcgag gcccttgtcc gtcgggtcgc gcagcagcac
     5341 gacctcgcgc ccggcgggcc tcagccccag atcggcggcc cgcgctgcga gcgcgcccgc
     5401 gagctcgatg taggactcgt ggaactcggc gcgggccatg gcccccgacc agcgcaggca
     5461 gcgctccgca gggaggtcga ggagctcgac ggcgggcgcc tcccggaaca tgccgtcgag
     5521 ctcgtcgatc cggccgagga gccggagcgc cgcgtcgagg cgcgaccgct cctgctcgag
     5581 gcgctgcttc tgctgggtga gcgtctcctc gagccggtcg ctggccgccg cgtcgatgta
     5641 caggcgcatc tcgccgagag gcacgccgag ccgacggagc aggcggatca tctcggccgc
     5701 gcggatggaa tggacgtcgt agtagcggta gccggtcgac tcgtcgacgt gagcgggcgg
     5761 caggaggccg cggtcggcat agaggcgcag cgcgcgatga ctcaggcccg tcatgcggga
     5821 gaagatgccg atcggcatca ggcgctgcgc gccggtatct ggggtcttgt cccgcgcctg
     5881 caagggatcc atacgcacac tctagcgttg actctcaccc gcgtcagtgt tccagactgc
     5941 gcccaccagt gccgcacggc ggcgctgacg acgcgtcagc tacggtcccg ggaggtccag
     6001 caccgtgaac gagatcatcg acgggctcgc cgcgctcgtc ggccgcgaca acgtcgcggc
     6061 cgacccggcg agcctagagg agtacgcgca ggacgagagc ttcgtcccgc gtctgacgcc
     6121 gtgggcggtc gtccgccccg gctcggccga ggaggtccag caggtcgtcg cctgggccaa
     6181 ccagacggcg acgccgctcg tgccggtcag ctccggcggc ccgcacttcc acggcgacac
     6241 ggtgccgagc gtgcccggcg cggtgatgct cgacctgcgc cggatggaca agatcctgcg
     6301 cgtcgaccgg cgcaaccgga tggccgtcat cgagccgggc gtcacgtacc cgcagctcca
     6361 ggccgcgctg gccgagcagg gcatgcgcgt cacgccgccg ctgtgcccgc gagcgaacaa
     6421 gtccgtggtc gcgagcctgc tcgagcgcca gccgacgctg atcccgcgct acaacttcca
     6481 cctgcccgag ccgctgcgca gctgcggcgt ggtgtggggc aacggcgacg tgatgttcac
     6541 cggcgaggcc ggcacgggcc cgctgtcgct cgaggcgcag tggcagggcg gcctcgtgca
     6601 ggccgacccc aaggggcccg gccagaccga cttcttccgc ttgctgaccg gggcgcaggg
     6661 cacgatggcc gtggtcacgt gggcggcggt caagtgcgag ctcgtcccca ccgcccacga
     6721 gtacctcttc gccgccgccg ccgagctcga cgagctggtc gactttgtct accgacttga
     6781 ccgcatccgg ctcggcgacg aggtcctcat cctcaacgac gcttacctgg cgcggctgct
     6841 caaggctgct ggggcggagg tcgcgccgga gc
//
LOCUS       DASUMX010000309         8887 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1413450, whole genome shotgun sequence.
ACCESSION   DASUMX010000309 DASUMX010000000
VERSION     DASUMX010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 8887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8887
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1413450"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="VFZ86_14540"
     CDS             <1..466
                     /locus_tag="VFZ86_14540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013125171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HEX5643536.1"
                     /translation="TGVQNKTRRYNVKIPAGVKDGSKIRIKGRGEAGLRGGPAGDLYV
                     RVRVETDDLFERRGDDLVVEVPVTVSEAALGATVKVPTPGGGRVSLKVPAGSQDGRTL
                     RLRGKGMPKLKGGHGSLLVKLRVQIPGKLTKDQKQLFEKLAATFPDPRSTAE"
     gene            487..900
                     /locus_tag="VFZ86_14545"
     CDS             487..900
                     /locus_tag="VFZ86_14545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX5643537.1"
                     /translation="MRRHHDDDRPLFMISVAAELAGMHPQTLRLYERRGLVRPQRTAG
                     KTRRYSERDVERLQRIQELTELGLNLAGVERVLAMEEQLETMRSQMHLLQQRLDEAAA
                     AMRLEVERVERSHRMELVPVSRSAVVHIGRRRRHA"
     gene            1005..3437
                     /locus_tag="VFZ86_14550"
     CDS             1005..3437
                     /locus_tag="VFZ86_14550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013756143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5643538.1"
                     /translation="MDVNKFSERAREALETAQGVVRRGPRNVLGTEHLLIGVLSLPGG
                     VCEQLLNRMGVDKGALMTRADRMAQNQPVGTSGPSGPDEMLYMTPRAKAALDLAVQEA
                     QKMGDEFVGTEHLLLGIFLEGEGPGSAILRDVGITEERLRDAMSQVRATGTDYEANAT
                     GESMLKKYTRDLTAMAREGKLDPVIGRDEEIKRVIQVLSRRTKNNPVLIGEPGVGKTA
                     IADGLAQKIVNADVPEILRGRRVLALDLGAMIAGSKYRGEFEERLKGALDEIQRSRDV
                     VLFIDELHTVVGAGAAEGAIDASNMMKPMLARGELQCVGATTLDEYRRHIEKDAALER
                     RFQPILVGEPTVEETVSILEGLRDKYEAHHKVKITDGAVREAAHLSHRYISDRFLPDK
                     AIDLLDEAASKLRIEATMLPPDLRDMELKLQELTREGAAAVQAQDYERAAQLKEQSDK
                     IQETFVGAKDAWLADKGIADATVDADDIAALVSEWTGIPVQRMLQEEADKLLEMESNI
                     HGRVIGQDRAVEAVSEAVRRARAGLKDPKRPVGSFLFLGPTGVGKTELARALAEFLFD
                     DEEALVRLDMSEYMEKHTVSRLTGAPPGYVGYDEGGQLTEAVRRRPYSVVLFDEIEKA
                     HPDVFNALLQILDDGRLTDGKGRTVDFKNTVVIMTSNLGSSVIGRNPLGFAAEAGGQT
                     REQTHEDMEKRLLDELRKSFRPEFLNRIDEVIVFDALTREQLTEVVDLLLTHLRKLVE
                     GQGMSLEVSAEAKEYVAEQGYDAEYGARPMKRAIQRLVENPLSSALLRGQFVEGDTIR
                     VGLADGGLVFTK"
     gene            3461..3724
                     /locus_tag="VFZ86_14555"
     CDS             3461..3724
                     /locus_tag="VFZ86_14555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643539.1"
                     /translation="MGKVRSVVFGGLIGAAAGVVLAPRHGESRREALGRLRLATRPGR
                     GAVRAFAGTPCSVEDRAAVEGQGGAPRAAGAAPAATGGGDDGG"
     gene            3717..4037
                     /locus_tag="VFZ86_14560"
     CDS             3717..4037
                     /locus_tag="VFZ86_14560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643540.1"
                     /translation="MDDARGGFDAARADSGFKELEVVAYIGEFKIIGIAHFGVGQRAS
                     SRRPSDYIRAFSDSRLTLSKVRIYGKGSQDLLETAPFIILNLDKVDFLYARDEPDAGD
                     LDGV"
     gene            4048..4737
                     /locus_tag="VFZ86_14565"
     CDS             4048..4737
                     /locus_tag="VFZ86_14565"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5643541.1"
                     /translation="MAYQLDLLVPTARFVFSVEDDAVEVLPEHRSPEAFVAYRDLRER
                     RPVVGMTTFPNTVLLRGPRTILVDPGLPLQNDPVLRALEARGLSAADVDLVVLTHAHL
                     DHAGGCAAVTAPVVLHELEVSSPYWPLVGGLLELFPLERLSGAGGELAPGLEWALTPG
                     HCAGHISLAADTARGRVVLCGDTIGPGRGEFDAMAPTGPDAEALLASWRRIRAWDPAL
                     IVAGHLPPFAP"
     gene            4813..5142
                     /locus_tag="VFZ86_14570"
     CDS             4813..5142
                     /locus_tag="VFZ86_14570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014142947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HEX5643542.1"
                     /translation="MPIYEYQCDECGNSFEVMQRMSDDPLTECEKCGGSLRKVLHPVA
                     IHFKGSGFYTTDYGKGSGKRPAAKESGADTSASDAGGGSSDGGDGSKTEKKAAGEGKS
                     KETQKAT"
     gene            5163..6470
                     /locus_tag="VFZ86_14575"
     CDS             5163..6470
                     /locus_tag="VFZ86_14575"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX5643543.1"
                     /translation="MERPTDEALRKAQTTLRGLVSDGPTHLPSLNLVADSIRLELEKS
                     GQVALLYVNLARYGRLEPVFGWRIVSEILDAVAANLESMVGNTLRRLDVVSDFTLTDD
                     AFIVLLSPPRSAAVIAEDDLAAVTRRVYERLQSLLLNDLAPGVYDRVHPSVGSAVLAA
                     DAGLTFEQTLQHGLALAMQAADEQAALYDQDLQATLADCVSRGDLEPLFEPVVDPAEG
                     EVIGYRSSVRGPFYSPLRLPDVLLDIARRSEVLASYGIVAREATVSAATGLEPDDLLF
                     LGCAAGELPNAAVVAVSEFYSLNPGLVPQHVVFDIDAGDLRHGTASTLRTLADVRDLG
                     FLLCISGLGAQFTALELIAEARPDFLCLDPALVAGLEHDLTAIEIVQLLVRFADRTGA
                     QLIATGVRDREQQHVLARNGVELFCGEAFARADTRPPRVTFPE"
     gene            complement(6507..7409)
                     /locus_tag="VFZ86_14580"
     CDS             complement(6507..7409)
                     /locus_tag="VFZ86_14580"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX5643544.1"
                     /translation="MTQRTHVSSVSDARRLTLRRGAVLLGSLAAVSALATLAGPGYLW
                     PLFLFPLVLGAVFFFELGSLAVTVWLGNFFVLYYSFAAPATPELVRQALLGTALFFLA
                     GLFLGRVQRRHRDTQRELSASTLTDRLTSLYNYGTFVDYLHNEVTKIDRYGGELCLLM
                     LDIDLFKQFNDRHGHEAGNELLRRLGATIGTLVRDADVAARYGGEEFAVLIRGDESQG
                     YELAERLRRAVETVTVEVRGGEHVRTTISAGVATYPAGAADEAGLIESADAALYESKR
                     RGRNRVTIFTGVSGERALPASLSA"
     gene            7565..8347
                     /gene="surE"
                     /locus_tag="VFZ86_14585"
     CDS             7565..8347
                     /gene="surE"
                     /locus_tag="VFZ86_14585"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEX5643545.1"
                     /translation="MTLQILLTNDDGIDAPGIAVLRRALDGLGEVTTIAPDRNTSAVA
                     RGITIDRALSLEPRAFGGGYDGLACDGTPSDCVRVALLGLRCPVPDLVVSGVNAGGNM
                     GADVTYSGTVGAAFEAALRGHPALAFSVEERAPGWLDEAEPVLRAMVGHVVDRGLPRH
                     SILNINLPDRPLAAITGIRPARLGGASRHDYVVLNGAGGRREHYLPCEHPASGEWVDT
                     DFDVVASGAVAVTPLRYDLLDPNLLSELAGWDLDLERLRAGL"
     gene            8334..>8887
                     /locus_tag="VFZ86_14590"
     CDS             8334..>8887
                     /locus_tag="VFZ86_14590"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX5643546.1"
                     /translation="MPDSDAAGFDPVVFDLDGTVVDTVELIVESFRYATRAVLREELP
                     DEVILHGVGQPLMAQMRRLSAEHAQELYDVYREYNHRRHDELIRGYEGVAEVLDGLRA
                     ASRRLGIVTSKSADTTQMAFRAVGLREHFEVVVTASDTAEHKPSPVPLHLCLERFGAS
                     AQGALYVGDSPVDIEAGRAAGMATA"
BASE COUNT         1335 a         3222 c         3157 g         1173 t
ORIGIN      
        1 cacgggcgtg cagaacaaga cccgccggta caacgtcaag atcccggcgg gcgtcaagga
       61 cggcagcaag atccgcatca agggcagggg agaggccggc cttcgcggcg gcccggccgg
      121 cgacctctac gtgcgcgtgc gcgtcgagac cgatgacctg ttcgagcgcc gcggcgacga
      181 tctcgtcgtc gaggtgccgg tgacggtgtc cgaggcggcg ctgggcgcga ccgtcaaggt
      241 gccgaccccc ggaggcggcc gcgtgtcgct caaggtgccg gccggcagcc aggacgggcg
      301 cacgctgcgc ctgcgcggca agggcatgcc caagctcaag ggcggtcacg gcagcctcct
      361 ggtcaagctg cgcgtgcaga tccccggaaa gctgaccaaa gaccagaagc agctgttcga
      421 gaagcttgcg gcgacgttcc ccgacccgcg ctcgacggct gagtgacacc cggcggagac
      481 ccggtcatga gacggcacca cgacgacgac aggccactct tcatgatctc cgtggcggcg
      541 gagctcgcgg gcatgcaccc gcagacgctc cgcctgtacg agcggcgcgg gctggtgcgc
      601 ccgcagcgca cggccggcaa gacgcgccgc tactcggagc gcgacgtcga gcggctgcag
      661 cgcatccagg agctcacgga actggggctc aacctggccg gcgtggagcg cgtgctggcc
      721 atggaggagc agctcgagac catgcgcagc cagatgcacc tgctccagca gcgcctggac
      781 gaggctgccg cggccatgcg ccttgaggtg gagcgcgtcg agcgctccca ccgcatggag
      841 ctggtgccgg tcagccgcag cgccgtcgtc cacatcggcc ggcgccgccg gcacgcctga
      901 gcgcgccggc tcgccggcag cggcggtcgg gtggtcccgg cagcgacgcc gccctgcagt
      961 cccgacccct tcgaagaacc cgacagcgag acaaggatcg acagatggat gtgaacaagt
     1021 tctccgagcg agcgcgcgag gccctcgaga cggcgcaggg cgtcgtccgc cggggcccgc
     1081 gcaacgtgct cggcaccgag cacctcctca tcggcgtgct cagcctgccc ggcggcgtct
     1141 gcgagcagct cctcaaccgc atgggcgtcg acaagggcgc cctgatgacg cgcgccgacc
     1201 gcatggcgca gaaccagccg gtcggcacga gcggtccctc agggccggac gagatgctct
     1261 acatgacgcc gcgcgccaag gccgccctgg acctcgccgt ccaggaggcg cagaagatgg
     1321 gcgacgagtt cgtcggcacc gagcacctgc tgctcggcat cttcctcgag ggcgagggtc
     1381 ccggcagcgc catcctcagg gacgttggca tcaccgagga gaggctgcgc gacgccatgt
     1441 cccaggtgcg cgccaccggc accgactacg aggccaacgc caccggcgag agcatgctca
     1501 agaagtacac gcgcgacctc acggcgatgg cccgcgaggg caagctcgac ccggtgatcg
     1561 gccgcgacga agagatcaag cgcgtcatcc aggtgctcag ccgcaggacc aagaacaacc
     1621 cggtcctgat cggcgagccc ggcgtgggca agacggccat cgccgacggg ctggcgcaga
     1681 agatcgtgaa cgccgacgtg ccggagatcc tgcgcgggcg gcgcgtgctc gcgctcgacc
     1741 tcggcgcgat gatcgccggc tccaagtacc gcggcgagtt cgaggagcgc ctcaagggcg
     1801 cgctcgacga gatccagcgc tccagggacg tcgtgctgtt catcgacgaa ctgcacaccg
     1861 tcgtgggcgc cggcgccgcg gagggcgcca tcgacgcctc caacatgatg aaaccgatgc
     1921 tggcccgcgg cgagctccag tgcgtgggcg ccaccacgct ggacgagtat cgccggcaca
     1981 tcgagaagga cgcggccctg gagcgccgct tccagccgat cctcgtcggg gagccgacgg
     2041 tcgaggagac cgtctccatc ctcgagggcc tgcgcgacaa gtacgaggcc caccacaagg
     2101 tcaagatcac cgacggcgcc gtgcgcgagg cggcgcacct gagccaccgc tacatcagcg
     2161 acaggttcct gccggacaag gccatcgacc tgctcgacga ggcggcgagc aagctgcgca
     2221 tcgaggcgac gatgctgccg ccggacctgc gcgacatgga gctcaagctc caggagctca
     2281 cgcgtgaggg cgcggcggcg gtccaggcgc aggactacga gcgcgccgcc cagctcaagg
     2341 agcagagcga caagatccag gagacgttcg tcggtgccaa ggacgcctgg ctggccgaca
     2401 agggcatcgc cgacgccacg gtggacgccg acgacatcgc cgcgctggtc agcgagtgga
     2461 ccggcatccc ggtgcagcgc atgctgcagg aggaggcgga caagctcctc gagatggaga
     2521 gcaacatcca cgggcgcgtg atcggtcagg accgcgccgt cgaggcggtg agcgaggccg
     2581 tccgcagggc gcgcgcgggt ctcaaggacc ccaagcgccc ggtcggctcg ttcctgttcc
     2641 tcggccccac cggtgtcggc aagaccgagc tcgcccgtgc tctcgccgag ttcctcttcg
     2701 acgacgagga ggcgctcgtg cgcctcgata tgagcgagta catggagaag cacaccgtgt
     2761 cccgtctcac cggcgcgccg cccggctacg tcggctacga cgagggcggg cagctcaccg
     2821 aggcggtgcg gcgtcgtccc tacagcgtgg tgctcttcga cgagatcgag aaggcgcacc
     2881 cggacgtctt caacgcgctg ctgcagatcc tcgacgacgg gcggctcacg gacggcaagg
     2941 gccgcaccgt ggacttcaag aacaccgtcg tcatcatgac cagcaacctg ggcagcagcg
     3001 tcatcgggag gaacccgctc gggttcgccg ccgaggccgg cggacagacc cgcgaacaga
     3061 cgcacgagga catggagaag cgcctgctcg acgagctgcg caagtcgttc cggcccgagt
     3121 tcctcaaccg gatcgacgag gtcatcgtgt tcgacgcgct cacgagagag cagctgaccg
     3181 aggtcgtcga cctcctgctc acccatctgc ggaaactcgt ggaggggcag ggcatgtcgc
     3241 tcgaggtgag cgccgaggcc aaggagtacg tggccgagca gggctacgac gccgagtacg
     3301 gcgcccggcc gatgaagcgc gccatccagc gcctggtgga gaacccgctc tccagcgcgc
     3361 tgctgcgcgg gcagttcgtc gagggtgaca cgatcagggt cggcctcgcc gacggcggcc
     3421 tggtgttcac caagtagccg cgggcggcta tagtccccgc atggggaagg tgcgcagcgt
     3481 ggtcttcggc ggcctgatcg gcgccgcggc cggtgtggtg ctcgcgccgc ggcacggaga
     3541 gtcgcggcgc gaggcgctcg gccgcctgcg cctcgccacg cgtcccggcc ggggcgcggt
     3601 ccgcgccttc gccgggactc cctgctcggt cgaggaccgc gccgcggtcg agggtcaggg
     3661 cggcgcgccc cgcgccgccg gagcggctcc ggccgcgact ggaggaggag acgatggtgg
     3721 atgacgcgcg cggcgggttc gacgcggccc gcgccgacag cgggttcaag gagctcgagg
     3781 tcgtggcgta catcggcgag ttcaagatca tcggcatcgc ccacttcggc gtcggccagc
     3841 gggcgagctc gcgacgtccc tccgactaca tccgcgcgtt cagcgacagc cgcctcaccc
     3901 tgtccaaggt ccgcatctac ggcaagggca gccaggacct gctcgagacg gcgccgttca
     3961 tcatcctgaa cctggacaag gtcgacttcc tctacgcgcg cgacgagccc gacgccggcg
     4021 atctcgacgg tgtctgaggc cgcgcccgtg gcgtaccagc tcgacctcct cgtcccgacg
     4081 gcgcggttcg tgttcagcgt cgaggacgac gccgtcgagg tgctccccga gcaccgctcc
     4141 ccggaggcgt tcgtcgcgta ccgcgacctg cgcgaacgcc ggccggtggt cggcatgacg
     4201 accttcccca acaccgtcct gctgcgcggg ccgcgcacca tcctggtcga tcccgggctg
     4261 ccgctccaga acgatcccgt gctccgcgcg ctcgaggccc gcggcctctc cgccgccgat
     4321 gtcgacctgg tggtgctcac gcacgcgcac ctcgaccacg ccggcggctg cgccgcggtg
     4381 acggcgcccg tcgtcctgca cgagctggag gtgtcctcgc cgtactggcc gctcgtcggc
     4441 gggcttctcg agctgttccc gctggagcgc ctcagcggcg ccggcggcga gctggcgccg
     4501 gggctcgagt gggcgctcac gcccggccac tgcgcggggc acatcagcct cgccgccgac
     4561 acggcgcgcg ggcgcgtcgt gctctgcggc gacaccatcg gtcccggccg gggcgagttc
     4621 gacgccatgg ccccgaccgg ccccgacgcg gaggcgctgc tcgcctcctg gcggcgcatc
     4681 cgcgcctggg acccggcgct catcgtcgcc ggccacctgc cgccgttcgc gccgtgaggc
     4741 cggccggccc gcgagcggcg gccggcctgt gctacactca ggcccctgcg accacgcccg
     4801 ggagacttct gcgtgcctat ctatgagtac cagtgcgacg aatgcgggaa ctcgttcgag
     4861 gtcatgcaga ggatgagtga cgacccgctc accgagtgcg agaagtgcgg tggctcactg
     4921 cgcaaggtgc tgcaccccgt ggccatccac ttcaaggggt ccgggttcta caccaccgac
     4981 tacggcaagg ggtcgggcaa gcgccccgcc gccaaggagt cgggcgcgga cacgagcgca
     5041 tcggatgcgg gcggaggttc gtccgacggc ggcgacggct ccaagaccga gaagaaggcc
     5101 gcgggcgagg gcaagtccaa ggagacgcag aaggcgacct gactcatcgg ggggtgagcc
     5161 gcgtggagcg accgaccgac gaggccctgc gcaaggcgca gaccaccctg cgagggctgg
     5221 tcagcgacgg ccccacgcac ctgccgtccc tcaacctcgt cgccgactcg atccggctgg
     5281 agctcgagaa gtcgggccag gtcgccctcc tgtacgtgaa cctggcccgg tacggccgcc
     5341 tcgagccggt gttcggctgg cgcatcgtct ccgagatcct cgacgcggtg gcggccaatc
     5401 tcgagtccat ggtcggcaac acgctgcgcc ggctggacgt cgtcagcgac ttcacgctca
     5461 ccgacgacgc gttcatcgtc ctgctctccc cgccgcggtc cgcggccgtg atcgccgagg
     5521 acgacctcgc ggcggtgacg cgccgcgtct acgagcggct gcagtccctg ctgctcaacg
     5581 acctcgcgcc gggcgtgtac gaccgcgtgc acccgtccgt gggctccgcc gtgctcgcgg
     5641 ccgacgccgg cctcacgttc gagcagaccc tgcagcacgg cctcgctctg gcgatgcagg
     5701 cggcggacga acaggccgcg ctctacgatc aggacctcca ggcgacgctg gccgactgcg
     5761 tctcgagagg ggatctcgag ccgctgttcg agcctgtcgt ggaccccgcc gagggcgagg
     5821 tgatcggcta ccgcagcagc gtgcgtggcc cgttctactc gccgctcagg cttcccgacg
     5881 tgctcctcga catcgcccgc cgctcggagg tgctggcctc gtacggcatc gtcgcgcgcg
     5941 aggctacggt gagcgcggcg acgggcctcg agccggacga cctgctgttc ctcggctgcg
     6001 ccgccggcga gctgcccaac gcggcggtcg tggccgtcag cgagttctac tccctcaacc
     6061 cggggctggt gccgcagcac gtggtgttcg acatcgacgc cggcgacctc aggcacggca
     6121 ccgcgtccac gttgcgcacg ctcgcggacg tgcgcgacct cgggttcctg ctctgcatct
     6181 ccgggctggg cgcgcagttc acggcgctcg agctcatcgc cgaggcacgc cccgacttcc
     6241 tgtgcctcga tcccgcgctg gtggccggtc tggagcacga cctcacggcg atcgagatcg
     6301 tgcagctgct cgtgcgcttc gccgaccgca ccggcgccca gctcatcgcc acgggcgtgc
     6361 gcgaccgcga gcagcagcac gtgctcgccc gcaacggcgt ggagctgttc tgcggagagg
     6421 cgttcgcccg cgccgacacg cggccgccgc gggtgacgtt cccggagtga gagggctgcg
     6481 cgtcgccgcg ccggcccggc tggtcgtcag gcgctcaggc tcgccggcag cgcgcgctcc
     6541 ccggagaccc ctgtgaagat ggtgacgcgg ttgcggccgc gccgcttgct ctcgtagagc
     6601 gcggcgtccg ccgactcgat cagtcccgcc tcgtcggccg cccccgccgg gtaggtcgcg
     6661 acgccggccg agatggtggt gcgcacgtgc tcccctccgc ggacctccac cgtcacggtc
     6721 tccacggcgc ggcgcaatct ctccgccagc tcgtacccct gcgactcgtc gccgcggatg
     6781 agcaccgcga actcctcgcc gccgtagcgc gccgccacgt cggcgtcgcg caccagcgtg
     6841 ccgatggtgg cgcccaggcg gcgcagcagc tcgttgccgg cctcgtgccc gtggcggtcg
     6901 ttgaactgct tgaagaggtc gatgtcgagc atcaggaggc acagctcgcc gccgtagcgg
     6961 tcgatcttgg tgacctcgtt gtgaaggtag tccacgaagg tgccgtagtt gtagaggctc
     7021 gtcagccggt ccgtgagggt ggacgcggag agctcccgct gggtgtcgcg gtgccggcgc
     7081 tggacgcgcc ccaggaacag cccggcgagg aagaagagcg ccgtccccag gagagcctgg
     7141 cggacgagct ccggagtggc cggagccgcg aagctgtagt acaggacgaa gaagttgccg
     7201 agccacacgg tcacggccag gctgccgagc tcgaagaaga agacggcgcc caggacgagg
     7261 gggaagagga acagcggcca gagatacccc gggccggcca gcgtggccag ggccgagacc
     7321 gcggccaggg atcccaggag cacggcgccg cgccgaagcg tgagccgcct ggcgtcgctc
     7381 acgctcgaga cgtgcgtccg ttgtgtcacg cggctccttc ccggcctcgc ctcggctctc
     7441 ctacgacggg cgccgtgcca atcctgccgt ccgggccgcg gaggcccgtc gccgcgacag
     7501 gctacgagga ggcgccgcgg cgtggctcgg gccacgtgag accggcttcc gctagaatcg
     7561 gtgcgtgacg ctgcagatcc tcctcaccaa cgacgacggc atcgacgccc cgggcatcgc
     7621 cgtactgcgc cgcgcgctcg acggcctcgg cgaggtgacg acgatcgccc ccgacaggaa
     7681 cacgagcgcg gtcgcccgag gcatcaccat cgaccgcgcg ctctcgctgg agccgcgggc
     7741 cttcggcggc ggctacgacg gcctcgcctg cgacggcacc ccgtcggact gcgtgcgcgt
     7801 cgccctgctg ggcctgcgct gcccggtccc cgacctcgtc gtctcggggg tcaacgccgg
     7861 cggcaacatg ggcgccgacg tcacctactc gggcaccgtc ggcgccgcct tcgaggccgc
     7921 cctgcgcggc caccccgcgc tcgcgttctc cgtcgaagag cgcgcgcccg gctggctgga
     7981 cgaggccgag cccgtgctgc gcgcgatggt cgggcacgtc gtcgaccgcg gcctgccgcg
     8041 gcacagcatc ctcaacatca acctgccgga ccgcccgctc gcggccatca ccggcatccg
     8101 cccggcgcgc ctgggcggcg ccagccggca cgactacgtc gtcctgaacg gcgcgggcgg
     8161 ccggcgcgag cactacctgc cgtgcgagca ccccgcgtcc ggtgagtggg tcgacaccga
     8221 cttcgacgtg gtcgcctccg gcgcggtggc cgtgacgccg ctcaggtacg atctgctcga
     8281 cccgaatctg ctgtccgagc tcgccggctg ggacctcgat ctggagcggc tccgtgccgg
     8341 actctgacgc cgccgggttc gaccccgtgg tgttcgacct cgacggcacc gtcgtcgaca
     8401 cggtcgagct catcgtcgag tcgttccgct acgccacgcg ggccgtgctg cgcgaggagc
     8461 tgcccgacga ggtcatcctc cacggggtcg ggcagccgct catggcgcag atgcgccggc
     8521 tctccgcgga gcacgcgcag gagctgtacg acgtctaccg cgagtacaac caccgccgcc
     8581 acgacgagct catccgtggc tacgagggcg tcgccgaggt cctggacggc ctgcgcgccg
     8641 cgagccggcg gctgggcatc gtgaccagca agagcgcgga caccacgcag atggccttcc
     8701 gcgcggtcgg cctgcgcgag cacttcgagg tcgtggtcac ggcgagcgac accgcggagc
     8761 acaagccctc gccggtgccg ctccacctct gcctggagcg cttcggggcg tccgcccaag
     8821 gcgcgctcta cgtgggggac agccccgtgg acatcgaggc cgggcgcgcc gccggcatgg
     8881 ccacggc
//
LOCUS       DASUMX010000310         4505 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1845397, whole genome shotgun sequence.
ACCESSION   DASUMX010000310 DASUMX010000000
VERSION     DASUMX010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4505
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1845397"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..381)
                     /locus_tag="VFZ86_14595"
     CDS             complement(154..381)
                     /locus_tag="VFZ86_14595"
                     /inference="COORDINATES: protein motif:HMM:NF025903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HEX5643547.1"
                     /translation="MREVGGKRAEGRSDLMPFIEIKLWEGRTREQKAEVARRVTEVMV
                     ESAGCTPESVTIAFEDFARSDWAERGELCDR"
     gene            689..865
                     /locus_tag="VFZ86_14600"
     CDS             689..865
                     /locus_tag="VFZ86_14600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643548.1"
                     /translation="MAVQLFPADGVTWIVSLPTGLAGDTIWPDMEWIEALAPVPGEEE
                     ASETTETLEEVRAA"
     gene            862..1797
                     /locus_tag="VFZ86_14605"
     CDS             862..1797
                     /locus_tag="VFZ86_14605"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEX5643549.1"
                     /translation="MTFEIRPYPEFSWSISRQRKLDQCPRGYFYTYYLGWNGWLQDAP
                     TEARVAYRLGKLTSLDALLGQQLDVRARELEAAARAGEELPTADALEARTREALRRLW
                     TRAREGRAAFEARPNKVTMLRSLYMGQDTQPETDRLNEKAGPSMRGLLATSHWERLGA
                     CGPDGCVVVPDFAHFVWDGVKVFAAPDLAYVQGGVLHVIDWKTGREDDSQALQVLLQM
                     WWALETLPELARAAADGSLEIRGYLEYVTAGRTQPVAAAPGFREQAAETVRAGVARMR
                     ALLADPERNIPLEMAAFERRESGLCRNCNFAPICA"
     gene            complement(1839..2648)
                     /locus_tag="VFZ86_14610"
     CDS             complement(1839..2648)
                     /locus_tag="VFZ86_14610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643550.1"
                     /translation="MTHGMDRGRDPGTKPARPPKDFQDYSDTRFGFRVWMPRRFEILP
                     DTIDPLARMIRGLDDMPEEERAKLQPRLPIGFLDPEVVGESGDGARQPLRVIEYEALR
                     GGEEPFGEERAEEMRAEIKVALPEILASAQMPGYKFLGTRETALGPLPALAFEYRWDG
                     VRPGFYGGDRARIVWALGPAAVFHVYHHCSGEVWGARRSELDAILASFEVLEPGEDDG
                     DAAARAAAVAAFQAAKTAGDSTAAAFQAGQAAYEAAQAGDSTVEGAGSTTE"
     gene            complement(2645..3313)
                     /locus_tag="VFZ86_14615"
     CDS             complement(2645..3313)
                     /locus_tag="VFZ86_14615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643551.1"
                     /translation="MSDARSGGRPEGLRRHEEPVYGYAVTVPAVFVALLNTVDPLARL
                     LRRLDDKELDEESRLTGSWPVGFADPEVVDDIGEGHHEPLRLLEFDVLTRPDPMSGAD
                     IAAMRAVVRDALPEALASRGLAGFDLLEVRDARLGPLDALAFEYQWAGPREAVDLTDR
                     ALVVWAPTPTAVYQVYYHCPAEAWDAWLPELEAILASFELVERRPESEGRPAPAPGEQ
                     GGAG"
     gene            complement(3335..3949)
                     /locus_tag="VFZ86_14620"
     CDS             complement(3335..3949)
                     /locus_tag="VFZ86_14620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643552.1"
                     /translation="MEYQTGAAAPLPEGFRRHEETLFGYSIAVPERFEPLGTTCDPVA
                     RSLRALDDATLEQEAERLASLPQGFWDPQAALTGGDGRPRPLRTLEMDCFMSERPLRQ
                     DKAAQMWFRMRRILPETLAEMGLPGYALVDQYDTALGPVAALGFEFRWDGTKAHAQAG
                     DHALVVWGLTAIQVFQLYYHCPAEEWDARLPELLEILKTFEPLG"
     gene            complement(4053..4382)
                     /locus_tag="VFZ86_14625"
     CDS             complement(4053..4382)
                     /locus_tag="VFZ86_14625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019551585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEX5643553.1"
                     /translation="MKYWRPWTPACGDIVRVRDATQRRRPALVLSPAAYSARVGLALV
                     CPIVSEPKGYPFEVALPPGLAVRGVVVADRVASIDWQACGARLAGAVGDEVVTAVRER
                     ILPLLAE"
BASE COUNT          603 a         1715 c         1559 g          628 t
ORIGIN      
        1 acgatgtcgc cggtgtcctt gtcgatctcc atgaccacct ggacgatctt ggagatctga
       61 ccggtgaccg agttcgacgg ggcacgcgcg tgccccacgt agaccgccga cctcatccgc
      121 gacctgccgg cgcctggcgc accggagcgc cgctcaccgg tcgcagagct cgccgcgctc
      181 cgcccagtcg ctcctcgcga agtcctcgaa cgcgatcgtc acggattcgg gcgtgcagcc
      241 ggccgactcg accatcacct cggtgacgcg ccgcgccacc tccgccttct gctcgcgcgt
      301 ccgtccctcc cagagcttga tctctatgaa cggcatgagg tcgctcctcc cctcggctcg
      361 cttgccccct acctctctca gttccggggg cgcgtgtcaa tgtccctgcg ggcgcctgcg
      421 aggacagcgc ggcgacggag tgctcgccgc aggggccagt cgctctcccg gtgggagtag
      481 tgcgggccac agcattcccg gccgcgcccg gccgcgagat tcccaagatc gtcgaggagg
      541 agccggccgc gtggcggcgc atcgtggcac ttctttcgtc cgacctccca tctatacttg
      601 cgcatcccac tgagatgcgg ctcgtgcgcg agggggcgga ggcgcgagtc ggcatccggc
      661 gtcctgtcgt ctaccatcgg aggtgccttt ggcggtacag ctgttccctg cagacggggt
      721 gacgtggatc gtctccctgc cgaccggcct ggcaggcgac accatctggc cggacatgga
      781 gtggatcgag gcgctggccc cggtccccgg cgaggaggag gcgagcgaga cgacggagac
      841 actggaggag gtgcgcgcgg catgacgttc gagatcaggc cctatccgga gttcagctgg
      901 tcgatcagcc ggcagcgcaa gctcgaccag tgcccgcgcg gctacttcta cacgtactac
      961 ctcgggtgga acggctggct gcaggacgcc cccacggagg cgcgcgtggc gtaccggctc
     1021 ggcaagctga cgtccctgga cgcgctgctg ggccagcagc tcgacgtgcg cgctcgcgaa
     1081 ctcgaggcgg ccgcgcgcgc cggcgaggaa ctgcccacgg ccgacgcgct ggaggcccgc
     1141 acacgcgagg cgctgcggcg actctggacg cgggcgcggg agggccgggc cgccttcgag
     1201 gcccggccga acaaggtcac catgctgcgc tcgctctaca tgggccagga cacgcagccc
     1261 gagaccgacc gcctcaacga gaaggccggc cccagcatgc gcggcctgct ggcgacgtcg
     1321 cactgggagc gcctgggggc ctgcggcccg gacggctgcg tcgtggtgcc cgacttcgcc
     1381 cacttcgtgt gggacggcgt caaggtcttc gccgcccccg acctcgccta cgtgcagggc
     1441 ggcgtgctgc acgtcatcga ctggaagacc ggccgcgagg acgactcgca ggcgctgcag
     1501 gtgctgctgc agatgtggtg ggccctcgag accctgccgg agctggcgcg ggcggccgcg
     1561 gacggcagcc tcgagatccg cggctacctg gagtacgtca ccgccggccg cacgcagccg
     1621 gtggcggcgg cccccggctt ccgcgagcag gccgccgaga ccgtgcgcgc cggcgtcgcc
     1681 cggatgcgcg ccctgctggc cgacccggag cggaacatcc cgctcgagat ggcggcgttc
     1741 gagcggcgcg agagcgggct ctgccgcaac tgcaacttcg cgccgatctg cgcgtaggac
     1801 gacacccaag ccctgcccgt gcttgagccg ccgtcgggct actcggtcgt cgagccggcg
     1861 ccctccacgg tcgagtcgcc cgcctgcgcc gcttcgtagg cggcctgccc cgcctggaac
     1921 gcggccgccg tcgagtcgcc cgccgtcttc gccgcctgga acgccgcgac cgccgcagcg
     1981 cgcgcggcag cgtccccgtc gtcttcgccg ggctccagca cctcgaacga cgcgaggatc
     2041 gcgtcgagct ccgaccggcg cgcaccccac acctcgccgg agcagtggtg gtagacgtgg
     2101 aacacggccg ccgggcccag cgcccacacg atgcgggcgc ggtcgccacc gtagaagccc
     2161 ggccgcacgc cgtcccagcg atactcgaag gccagcgccg gcagcgggcc gagcgcggtc
     2221 tcgcgggtcc cgaggaactt gtagcccggc atctgcgcgc tcgccaggat ctcgggcagg
     2281 gccaccttga tctccgcccg catctcttcg gctcgctcct cgccgaacgg ctcctcgcct
     2341 ccgcggagcg cctcgtactc gatcacgcgc agcggctgcc tggccccgtc cccggattcg
     2401 ccgacgacct cggggtccag gaaaccgatg ggcagccggg gctgcagctt cgcccgttcc
     2461 tcctccggca tgtcgtcgag gccgcggatc atgcgcgcca gcggatcgat cgtgtccggg
     2521 aggatctcga agcgcctggg catccacacc ctgaagccga agcgcgtgtc ggagtagtcc
     2581 tggaagtctt tcggcggccg cgccggcttc gtgccggggt cccggccgcg gtccatcccg
     2641 tgggtcatcc tgcacctccc tgctcgccgg gcgccggtgc cggccttccc tcggactccg
     2701 gccgccgctc gacgagctcg aacgacgcga ggatggcttc gagctccggc agccacgcgt
     2761 cccacgcctc ggccgggcag tggtagtaga cctggtagac cgcggtaggc gtcggcgccc
     2821 agaccacgag cgcgcggtcg gtcagatcca cggcctcccg aggcccggcc cactggtact
     2881 cgaacgccag ggcgtcgagc gggccgagcc tcgcgtcgcg cacctcgagg aggtcgaagc
     2941 cggccaggcc gcgcgacgcg agcgcctccg gcagcgcgtc gcgcaccacc gcgcgcatcg
     3001 ccgcgatgtc ggcgccactc atcgggtcgg ggcgcgtgag cacgtcgaac tcgagcaggc
     3061 gcagcggctc gtgatggccc tcgccgatgt cgtccaccac ctcgggatcg gcgaacccga
     3121 cgggccagct gccggtgagc ctgctctcct cgtccagctc cttgtcgtcg agccggcgca
     3181 ggagccgcgc cagcgggtcc acggtgttga ggagggcgac gaacaccgcc ggcaccgtca
     3241 ccgcgtatcc gtagaccggc tcctcgtgcc ggcgcagccc ctccggccgg cccccgcttc
     3301 gcgcgtcgct catgctcact ccatctcgcc ggggtcagcc cagcggctcg aacgtcttga
     3361 ggatctccag cagctcgggc agccgggcgt cccactcctc cgcggggcag tggtagtaca
     3421 gctggaacac ctggatcgcc gtcagccccc acacgacgag cgcgtggtcc ccggcctgcg
     3481 cgtgcgcctt cgtcccatcc cagcggaact cgaaccccag cgcggcgacg ggaccgagcg
     3541 ccgtgtcgta ctggtcgacg agagcgtagc cgggcaggcc catctcggcg agcgtctcgg
     3601 ggaggatgcg gcgcatgcgg aaccacatct gcgccgcctt gtcctgccgc agcggacgtt
     3661 cgctcatgaa gcagtccatc tcgagggtcc gcagcgggcg cggccggccg tcgccgccgg
     3721 tgagcgcagc ctgcggatcc cagaagccct gcggcaggct cgccagccgc tcggcctcct
     3781 gctccagcgt cgcgtcgtcg agcgcgcgca gggagcgcgc gaccgggtcg caggtcgtcc
     3841 cgagcggctc gaaccgctcc ggcacggcga tgctgtagcc gaacagcgtc tcctcgtgcc
     3901 ggcggaagcc ctcgggcagc ggcgccgcag cgccggtctg gtactccacg ccggcctcct
     3961 ccctcgacga cgacccgccg ccggcggcag ctgcacgccg gcggaccctg aaggggcgag
     4021 tatacgcggc cggcgtgcct ggtccggccc cctcactccg cgagcagcgg caggatccgc
     4081 tcacgcaccg ccgtgaccac ctcgtcgccg accgcgccgg ccagcctggc gccgcatgcc
     4141 tgccagtcga tgctcgcgac ccggtccgcg acgacgactc cgcgcacggc aagtccaggc
     4201 ggcaacgcga cctcgaaggg gtagcccttg ggctcgctga cgatcggaca cacgagggcg
     4261 agccccaccc tggcgctgta ggcggccggc gacagcacga gggccggccg ccgccgttgc
     4321 gtcgcgtccc ggacccttac gatgtcgccg cacgccggcg tccagggccg ccagtacttc
     4381 atctctcgcc ggtctccagc ccgcccgcgg ggcgccagtt gaggtccgtg tgcttgttct
     4441 gctgcgtcac ttccgcgagc agttcctcca gggtcggggc cgaacgcagc ggctcgatca
     4501 cgatc
//
LOCUS       DASUMX010000311         6959 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_982848, whole genome shotgun sequence.
ACCESSION   DASUMX010000311 DASUMX010000000
VERSION     DASUMX010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 6959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6959
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_982848"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="VFZ86_14630"
     CDS             <1..383
                     /locus_tag="VFZ86_14630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010270972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEX5643554.1"
                     /translation="AGLTVDMADIPDTFMTLAAIAPLATSPVTVTGIGNVRLKESDRI
                     AAMEDNLRRTGVRTESGADWLRVFPCTPRGASVDPRGDHRIAMAFSVLGLRVPGIVIE
                     DPSCVAKTCPTFFDLWAALEQGTT"
     gene            450..1499
                     /locus_tag="VFZ86_14635"
     CDS             450..1499
                     /locus_tag="VFZ86_14635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643555.1"
                     /translation="MDDETTGRLPDEDDELTRVTPPDDATRVMPAAGEGETQVMPRAD
                     AGADTGATRVMPAAGQAPPPPPSQPTLLMTHGRPSSDRGVPWWVWLLVVLAVVAVAAA
                     LWYFYLRPTDATTAGEEFIGHWSPQSGNGGGLVIKQSGEQFQITQYDDQLEKVGTTTA
                     DLVDDQLQLSVQASAIGLQGVTGAVQGTLTHESAQDLLRLQFAAGDVTIEPVYFVRVD
                     TLLPGVPSPTPSPTASPTPTASPSPTITATPSPSATGSPTADQEAVANIARLQVGIVA
                     WAADNNNLYPPPQDVVEGGGLSQYVTPWPANPFTGEPMAPGTAPGDYVYEQLSGGQAY
                     KLTGYLSNGLTYAVP"
     gene            1496..2440
                     /locus_tag="VFZ86_14640"
     CDS             1496..2440
                     /locus_tag="VFZ86_14640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone reductase"
                     /protein_id="HEX5643556.1"
                     /translation="MTAVRAVRVHEFGGPDVLVLDEVPQPVPVEGEVLVRIQAAGVNP
                     FETYARAGSYSNLPALPYTPGCDGAGVRVDTGERVYVTDSLSGTYAEYALCPAEAVRP
                     LPDDLSYAQGAAVGVPYFTAYRALLQRAAAKDGERVLVHGASGGVGIATVQLAVAAGL
                     EVTGTAGSEAGRELVAAQGVAQVLDHGDPAHVDAALALTGGAGFDVIIEFLANVNLGS
                     DLKLLAKRGRVVVVGSRGTVEVDARDLMNAEGAVLGMLTPNARRDEVAAALGAVDAGL
                     RSGVLKPVVGREVPLAEAARAHRLIMERPALGKMVLVP"
     gene            2504..3880
                     /locus_tag="VFZ86_14645"
     CDS             2504..3880
                     /locus_tag="VFZ86_14645"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013474084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX5643557.1"
                     /translation="MTDYRARSMWLDGIPGELTPRPPLPGPADVDVAVVGAGFTGLWT
                     AYYLKKADPHLRIAVLEREVAGFGASGRNGGWCHTTFPGSRELAARTHGRQAVIDQQR
                     ALHRTVYEIARVVQDEGIDARFHLGGQLDLATTPVQLLRLREAVEYERSWSFGEDDYV
                     ALGAAEMRERIKVAGCLGAGYTPHGAAVDPARLARGLAGVVEGLGVPIYERTPVTRLE
                     PHVATTAYGDVRADVVVRATEAFTPELPGHERTLVPIYSLMIGTEPLPDSFWDEIGWA
                     GHEVFGDFRFLIYYAMHTQDGRIAIGGRGAPYHFGSRLSERFERDPAVRDHLRALLGE
                     LFPAIGDFTVTHHWGGAIAAARDWYTSVGLDRATGMAWAGAYVGDGVSTTNLAGRTLR
                     DLILGQESELTGLPWVDHRSRLWEPEPLRWLGVNAALRAMGSADDAEARTGRPSRRAT
                     CVKKLIGA"
     gene            complement(3903..4286)
                     /locus_tag="VFZ86_14650"
     CDS             complement(3903..4286)
                     /locus_tag="VFZ86_14650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643558.1"
                     /translation="MKRLTSILLVALLAFGAAGALAGCGLREEAAEKTTGQIDVARDA
                     TLKAQLMMIKTGIQAFAVTNGAAPPDASQATLGGVVSPWPTNPFSNAPMKPGEGVGDY
                     VYAPGAGASYTLTVNLSDGSTYTAP"
     gene            4395..4931
                     /locus_tag="VFZ86_14655"
     CDS             4395..4931
                     /locus_tag="VFZ86_14655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643559.1"
                     /translation="MGRPSRLRQPRLLRRDWVGDGILTLLAGAVLVIAVFLPWANEDL
                     LGDVNYSLSSSGDINGVLQTQWGAPALALALTAVALGAAVTLTRPRRLSWLLGLAVAA
                     CGAGVFAVAQDAASQIAFFDPGLGLYLTTLVGVLLVPIGAAAAMVAWFVARADAAPLA
                     AADEAPTAPPAPENAPPS"
     gene            complement(4891..6252)
                     /gene="mtbB"
                     /locus_tag="VFZ86_14660"
                     /pseudo
     CDS             complement(4891..6252)
                     /gene="mtbB"
                     /locus_tag="VFZ86_14660"
                     /inference="COORDINATES: protein motif:HMM:NF021053.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="[dimethylamine--corrinoid protein]
                     Co-methyltransferase"
     gene            6420..>6959
                     /locus_tag="VFZ86_14665"
     CDS             6420..>6959
                     /locus_tag="VFZ86_14665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643560.1"
                     /translation="MTTTHMFRRTLILGATLLATVCLGAGAAAPARAASPDAQMRQSL
                     LVLRGLIDREGAARYFAYPAKSTVRYGHLGGSAWPFDPWTGARLSAGTGRGHYQYWIS
                     GTRRRYRLTGYLGDGRTVVLTGGMPRSIKLAYDHRGEEGINLIRQYVEDYAAEHSGRY
                     PLPAEVDGDGGAGSGHYWPS"
BASE COUNT          936 a         2605 c         2427 g          991 t
ORIGIN      
        1 tggccggcct caccgtcgac atggccgaca tcccggacac cttcatgacg ctggccgcca
       61 tcgccccgct ggccacgtcg ccggtcacgg tcaccggcat cggcaacgtg cgcctcaagg
      121 agtccgaccg catcgccgcc atggaggaca acctgcgccg gaccggggtg aggacggagt
      181 ccggcgccga ctggctgcgg gtcttcccct gcacgccccg gggcgcgtcc gtggacccgc
      241 gcggagacca ccgcatcgcc atggccttct cggtgctggg gctgcgcgtc cccgggatcg
      301 tgatcgagga tccgtcgtgc gtggccaaga cctgccccac cttcttcgac ctctgggcgg
      361 cgctggaaca gggaaccacc tgaagagggc aaagtagtag agtcgaacca ccgaaggccg
      421 acgagcgtca cccggcgggg agggcgagca tggacgacga gaccacaggc agactgcccg
      481 acgaggacga tgaactgacc cgggtgacgc ctcctgacga cgccacccgg gtcatgccgg
      541 cggcgggcga gggcgagacg caggtcatgc cccgcgccga cgcgggcgcc gacacgggcg
      601 ccacccgggt catgccggcg gcggggcagg ctccacctcc gccgccgagc cagcccaccc
      661 tgctcatgac gcacgggcgg ccatcctccg accggggcgt gccctggtgg gtctggctcc
      721 tggtggtgct cgcggtcgtc gcggtcgcag ccgccctctg gtacttctac ctgcggccca
      781 ccgacgcgac caccgccggc gaggagttca tcggccactg gtcgccgcag tccggcaacg
      841 ggggcggcct cgtcatcaag cagtccggcg agcagttcca gatcacccag tacgacgatc
      901 agctcgagaa ggtcggcacc accacggccg acctcgtgga cgaccagctg caactgagcg
      961 tgcaggcgtc cgccatcggc ctgcagggcg tgaccggcgc cgtgcagggg acgctcacgc
     1021 acgagagcgc ccaggacctt ctgcggctgc agttcgcggc cggcgacgtg accatcgagc
     1081 cggtgtactt cgtgcgcgtc gacacactgc tccccggggt cccatcgccg actccgtcac
     1141 ccaccgcctc gccgaccccg accgcgtcgc cgtcgcccac catcaccgcc acgccgagcc
     1201 cgtcggccac cggctcgccc accgcggacc aggaggcggt cgccaacatc gccaggctgc
     1261 aggtgggcat cgtcgcctgg gcggcggaca acaacaacct gtatccgccg ccgcaggacg
     1321 tcgtcgaggg cggcggcctc tcgcaatacg tgactccctg gcccgccaat ccgttcaccg
     1381 gcgagcccat ggcgcccggg acggcgccgg gtgattacgt gtacgagcaa ctcagcggcg
     1441 gtcaggccta caagctcacc ggctacctca gcaacgggct gacgtacgcg gtgccgtgac
     1501 ggcagtgcgc gccgtccgcg tccacgagtt cggcggtccc gacgtcctcg tcctcgacga
     1561 ggtgccccag cccgtgcccg tggagggcga ggtcctcgtc cgcatccagg cggcgggggt
     1621 caaccccttc gagacctacg cgcgcgcggg cagctacagc aacctgcccg cgctgcccta
     1681 cacgcccggc tgcgacgggg cgggcgtccg cgtcgacacg ggcgagcgcg tctacgtgac
     1741 cgactcgctc agcgggacgt atgcggaata cgcgctgtgc ccggccgagg ccgtccgtcc
     1801 gctgccggac gacctctctt acgcacaggg ggcggcggtc ggcgtcccgt acttcaccgc
     1861 ctaccgcgcc ctgctccagc gggcggccgc aaaggacggc gagcgggtcc tggtccacgg
     1921 cgccagcggc ggagtcggca tcgccaccgt ccagctggcg gtcgccgccg gcctcgaggt
     1981 caccgggact gccggcagcg aggccgggcg cgaactggtc gccgcccagg gcgtcgcgca
     2041 ggtgctcgac cacggcgacc cggcacacgt cgatgcggcg ctggcgctca ccgggggcgc
     2101 cggattcgac gtcatcatcg agttcctcgc caacgtgaac ctcggcagcg acctgaagct
     2161 gctggccaag cgcggtcgcg tggtggtggt gggcagcaga ggcaccgtgg aggtcgatgc
     2221 ccgcgacctc atgaacgccg agggcgcggt gctcggcatg ctcacgccca acgcacggcg
     2281 cgacgaggtg gcggccgcgc tcggcgccgt ggacgccggc ctgcgcagcg gcgtgctcaa
     2341 gcccgtggtc ggccgcgagg tgccgctggc cgaggccgcg cgagcccatc gcctgatcat
     2401 ggaacgcccg gcgctcggca agatggtgct cgtcccgtag gcctcactgc gccgccgccc
     2461 gccgggtttg gtagcatcgc gacaccctcc aaggagcccg aacgtgaccg actaccgcgc
     2521 ccgcagcatg tggctcgacg ggatccccgg cgagctcacc ccgcgccccc ccctgcccgg
     2581 tcccgccgat gtcgacgtcg ccgtcgtcgg cgccgggttc accggcctgt ggaccgccta
     2641 ctacctgaag aaggcggacc cgcacctgcg catcgcggta ctggagcgcg aggtcgccgg
     2701 cttcggcgcc tccgggcgca acggcggctg gtgccacacg accttccccg gctctcgcga
     2761 gctcgcggcg cggacgcacg gccgtcaggc cgtgatcgac cagcagcgcg cgctgcaccg
     2821 gacggtgtac gagatcgcgc gcgtcgtgca ggacgagggc atcgacgcgc gcttccacct
     2881 cggcggccag ctggacctcg cgaccacgcc ggtccagctg ctccgcctgc gcgaagcggt
     2941 cgagtacgag cgctcctgga gcttcggcga ggacgactac gtcgcgctcg gcgcggccga
     3001 gatgcgcgag cgcatcaagg tggccgggtg cctcggcgcc ggctacacgc cgcacggcgc
     3061 cgccgtcgac ccggcccgtc tcgcgcgcgg gctggccggc gtggtggaag ggctcggggt
     3121 gcccatctac gagcgcaccc ccgtcacccg tctcgagccg cacgtcgcga ccaccgcgta
     3181 cggcgacgtg cgcgccgacg tcgtggtgcg cgccaccgag gcgttcacgc ccgagcttcc
     3241 cggccacgag cggaccctgg tgccgatcta ctcgctcatg atcggcaccg agccgctgcc
     3301 cgacagcttc tgggacgaga tcgggtgggc cggccatgag gtgttcggcg acttccgctt
     3361 cctcatctac tacgccatgc acacccagga cggccgcatc gccatcgggg ggcgcggcgc
     3421 cccgtatcac ttcggctcgc ggctgagcga gcggttcgag cgcgaccctg ccgtccgcga
     3481 ccacctgcgc gcgctgctcg gcgagctctt cccggcgatc ggcgacttca ccgtcaccca
     3541 ccactggggc ggcgcgatcg cggcggcgcg cgactggtac acctcggtcg gcctggaccg
     3601 cgccaccggc atggcctggg cgggcgccta cgtcggggac ggcgtctcga cgaccaacct
     3661 cgccggccgt accctgcgcg acctgatcct cgggcaggag tccgagctca cggggctgcc
     3721 gtgggtcgac caccgctcca ggctctggga gcccgagccg ctgcgctggc tgggagtcaa
     3781 cgcggcgcta agggcgatgg ggagcgccga cgacgcggag gcgaggaccg gccggccgag
     3841 caggcgggcc acctgcgtca agaagctcat cggcgcctga gcctgcggcc ggcgcgctca
     3901 cgtcacggcg ccgtgtacgt gctcccgtcg gagagattga cggtgagggt gtacgaggcg
     3961 ccggctcccg gcgcgtagac gtagtcgccc acgccttccc ccggcttcat cggcgcgttg
     4021 ctgaaggggt tggtgggcca cgggctgacc acgccgccga gcgtcgcctg ggaggcgtcg
     4081 ggcggcgctg cgccgttggt gacggcgaac gcctggatgc ccgtcttgat catcatcagc
     4141 tgcgccttga gggtcgcgtc cctggcgacg tcgatctgcc cggtcgtctt ctccgcggct
     4201 tcctcccgca ggccgcagcc ggcgagcgct ccggcggcgc cgaacgccag cagcgcgacg
     4261 agcaggatgc tcgtgagtcg cttcatcggt gaggtcctcc ctggtcgatg gcttccctat
     4321 cggcacgctt cgccgcaccc tcgagcgctc ctcggcacgt tcaggaagag ggtcgggcgg
     4381 gcgatactgg ggacatggga cgcccgagcc gcctacgaca gcctcgactg ctgcgccgcg
     4441 actgggtcgg cgacgggatc ctgacccttc tcgccggcgc cgtcctggtc atcgcggtgt
     4501 tcctgccttg ggccaatgag gacctgctgg gcgacgtgaa ctacagcctg tcgtcgtccg
     4561 gggacatcaa cggcgtgctg cagacgcagt ggggggcgcc ggccctcgcg ctcgcgctga
     4621 ccgccgtggc gctcggcgcc gcggtgacgc tgacgcggcc gcggcgcctc tcctggcttc
     4681 tcggcctggc agttgcggca tgtggcgccg gcgtcttcgc cgtggcccag gacgccgcgt
     4741 cgcagatcgc cttcttcgac cccggcctcg ggctctacct caccaccctc gtcggggtcc
     4801 tgctggtccc catcggagcg gccgccgcga tggtcgcctg gttcgtcgcc cgcgccgacg
     4861 cagcgcccct cgcggccgcc gacgaggctc ctacagcccc gccagctccc gaaaacgctc
     4921 caccgagctg atcggcacgc ccagcagctc ggcgatcctg aacttcgccg tcatcgcgtt
     4981 gggctcgccc gggtgcaggt tggagtggtc gaggatccgc cccaggccct tctcgccgcg
     5041 gatctgctcc atcgcgtagc tgtcgctgag atcgcggacc gtgcagccca gcttgtccgc
     5101 gacgtaggct ttcgcgtccg ggaggcgcat cccgcgggcc atctgcatgc gcgcgaccag
     5161 gtctccggcc gtgcgcatcc cgcccatgcc cgcggcgagc gcgtgggtga cgtccatgcc
     5221 tctggagtca cccgagccca cctacaaccc gtcgcaacga aggatgtcga cgtgcgcctt
     5281 ggccgcccgc gacgccgcgt cctcgggcgg gtagccgttc accggcacgc cgccgacgcc
     5341 ctcgcccacg ttggggtgca cggggatgcg ggcgacgtcc atgcacggct tcgtcaccgt
     5401 gatggagtac gccgcgttcc aggcgcagct cttgccggtg cggatgttga ccgccggccc
     5461 gaagatggtg gcgccggcgt cctggcacac ctcgagctgg tccctgggcc agaggccggc
     5521 gagccgccgg ccctcccact cgagctcgcc gtggacgccg atgacgaact cgccggccat
     5581 gccgacctcg atgccggcgt cgggatactt gccccgcaga tgtcgtaccg ctcgcagggt
     5641 gctgaagaag tcggcgtcgc cggcgccgcc ggtggtgtcg aagtccatgc cgtcggcgcc
     5701 ggcgtcgagc atcccctcgc ccacgtagat gaagtccttg gtggcgtggt ccatggcctc
     5761 gtcgatggcc gcgcgggcgc cggcgatgtc cccgcgcggc agcagctcga accagttgtc
     5821 cgccggcccg tcgggcttgc tgtagaaggc gaggttgggc tgcgccccgt actgcaccgg
     5881 cgccgtgcac atcagctgca cgttctgcat cacctgctgc tggaacggaa ggatcgtctt
     5941 gatcgccttg tagctgtact cgatgtgcca gagctcgagg atgtcctggc ccaggcgctg
     6001 ctcgctctcg agcaggtcga ggaggtcggt tcccgtcatc ttgctgccgc cgccgtcgca
     6061 gctgaggacg acctggtcgc cgatgtcgac ggcggtgaag gcggcacgag aggcgtagat
     6121 gtcgaggagg tggtcgagct cgtcgggggc gagcggcggc accttggcct tgcgcgccgc
     6181 ctcctgggtg gctgtctcga tgtccgcgcg cagctcgctg cgcgtcagct cgaccatgaa
     6241 gccatcgccc atgcgggtcg ggaccttcgc ttccaccggc agctccttgt gcgacgagtc
     6301 gcgccgcacc ggccggaaag gccccgcggc gcccgccgcg aatcgtatca gcagaccgcc
     6361 ccatgaaggt ggcgcgcttg accggtcccg tgcccgcggc cacactggag tcgaaagggg
     6421 tgaccacgac gcacatgttc cgacgcacgc tcatcctcgg cgcgacgctg ctcgcgacgg
     6481 tctgtctcgg cgcgggcgct gccgcgccgg cccgcgccgc ctcccccgac gcccagatgc
     6541 gccagtctct cctcgtgctc cgcggcctga tcgaccgcga gggcgcggcg cgctacttcg
     6601 cgtacccggc gaagtccacg gtccgctacg gccacctcgg cggctccgcc tggccgttcg
     6661 atccctggac gggcgcccgg ctcagcgcgg gaaccgggcg cggacactac cagtattgga
     6721 tcagcggcac gcggcggcgc taccgactga ccggctacct cggcgacggt cgcacggtcg
     6781 tcctgaccgg tggcatgccg cggtccatca agctcgcgta cgaccaccgc ggcgaagagg
     6841 gcatcaacct catccgccag tacgtcgagg actacgccgc cgagcacagc ggccggtacc
     6901 ccctccccgc cgaggtcgac ggcgacggcg gcgccggcag cggccactac tggcccagc
//
LOCUS       DASUMX010000312         6817 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1126793, whole genome shotgun sequence.
ACCESSION   DASUMX010000312 DASUMX010000000
VERSION     DASUMX010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 6817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6817
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1126793"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2917)
                     /locus_tag="VFZ86_14670"
     CDS             complement(<1..2917)
                     /locus_tag="VFZ86_14670"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5643561.1"
                     /translation="MYLKALRLKGFKSFPKQTELLFEPGVGVIIGPNGSGKSNLADAV
                     IWALGEQSPTTVRGSSMQDVIFAGSDGRRASGAAEVELTFDNADGALPLPTAEVSVMR
                     RVARDGSSQYSINQSACRLTDVVELLAQVGLGKELHSIIGQGKVESFLAGKPEDRRSQ
                     IEEAAGLGAYKRRRERAELKLREVRRNLERALLLEREVGSQLAPLRRQATAAEQLRTV
                     ESEIAEARGRLLTGDLLALDADLQARRDDLAAIDAERATHEQGLARVAAARAREEEAF
                     ARKLAERERSARRLLRARVLDGRLESVRRLAEQRRHLLDEVERAATAERERLVAELAG
                     RPEERDDDAWPQEERRLAGELEAAETEYAGAAAELEAARAASTERRVALDRLAVERES
                     ALSTAARLERRGEALGGEQERLAAQHEAMSAEVTGKAGEEREAAEAEAAAREQLRTTE
                     AAAAAAAAAVADAARDVTTAEDAHRATLMERRALEAEADHLRAALLDMEDVGGEVLEV
                     AGEFPGTVSLAGSVSCEPGYERALAAALAQLSGALAVPAGVDQWSLLDALKRAGIGLV
                     RLVVPARRRPSTAFPGAAPLADKVSFGDQGLEGALADVVIVDDLRAVPAEFTGLAVTR
                     AGEYYRPADGQIGLAGGVPAALLLERRASLERLGEKLAAVSAREAREEAAVQLAVSRL
                     TAAREAGDATAAADREARIAAETAERALAQVRSRRRDLEDHLQRDRRAVEGIAAELAE
                     AASSKEAADATAANALLQSEQLRPAVEEAEAALHRSEAAHSAALALVTRRRVELEERR
                     AAAARVAEQREAARRRAAADRERLEELERRLAGIPDVRAACATVEARTGALRAHSTRL
                     IAQLDVGDEEGAGLERGELRKLADDEAQLRRGLENAGERRTTIQVALARLEDRRTELA
                     ASLEEVSERIDQAGFAPPADAAEETGLRERVERLSRRRERIGPVNPLAEAECAELSER
                     "
     gene            complement(2919..3779)
                     /gene="rnc"
                     /locus_tag="VFZ86_14675"
     CDS             complement(2919..3779)
                     /gene="rnc"
                     /locus_tag="VFZ86_14675"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEX5643562.1"
                     /translation="MTQRLLTLAQQLDQATLRQVFTHTSWAPDRVHSYERLEFLGDSV
                     LSLCITTELYRRFPEFAEGHLARLRAYIVSRATCARVAAKLGLGKLLRQYAGQGGEGG
                     DAAQLQTNQNVLADLTESLIGAVYLTFGYEAVRPAVIEVFEEHIVFAESSYIDHKTEL
                     QEFLARGGQSVAYKVLGFSGPPHNREFEIEAVVDGDSLGRGFGTSKKRAEQEAAAEAL
                     HELQTHARKTARKARLRGSRSRRPQASETGAADIPEAPPVAEAAPVGEAAPHEADASA
                     AGPAGATEVG"
     gene            complement(3865..4110)
                     /gene="acpP"
                     /locus_tag="VFZ86_14680"
     CDS             complement(3865..4110)
                     /gene="acpP"
                     /locus_tag="VFZ86_14680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015123555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX5643563.1"
                     /translation="MTREEVFEHVKGILVETLSVDEEKVTMDARFQEDLETDSLDLVE
                     LVMTMEEKFGIKITDEEAADIKTVGDAVDFVMTRVDA"
     gene            complement(4142..5218)
                     /gene="plsX"
                     /locus_tag="VFZ86_14685"
     CDS             complement(4142..5218)
                     /gene="plsX"
                     /locus_tag="VFZ86_14685"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEX5643564.1"
                     /translation="MRGSRNRPYTSPHWVTDAWSAVTDDRVVRVVVDAAGGDNAPGEI
                     VKGALDACGPQLNVILVGDEPRVRALLPEGVQHVSVVHAPDVIGYHEEPATAARARTG
                     SSIVVGLKMVHNGEAEAFVSAGNTGAVVAASVLYVRRIPGVKRPAICTIMPAMPAPVA
                     FLDVGANAEVRAEFLRQFAIMGQAFAREVMGIPEPTIGLLSIGEEPTKGTPDVIEAHQ
                     LIAGDHRISHFYGNIEGRDIMNRLVDVIVTDGFTGNVGLKTIEGSARAILGALKATVM
                     VSAKTKLGGLLLQKDLQVLKDALNPEEFGGALLVGMNAPVVIAHGNSKARGIAQAVRM
                     GRRGAVSDLLPTIARELGTQTAAV"
     gene            complement(5215..6279)
                     /locus_tag="VFZ86_14690"
     CDS             complement(5215..6279)
                     /locus_tag="VFZ86_14690"
                     /inference="COORDINATES: protein motif:HMM:NF024891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransacetylase family protein"
                     /protein_id="HEX5643565.1"
                     /translation="MTRSLYIASTQGSGGKTTIAVGLCMALRERGLDAGYFKPVGTLA
                     AHADGVLLDEDAEFVAEVLELEDDLTDICPVVLDEDALHDVLSGTEVDAMSRVKAAYE
                     RIRAGRDIVVCEGLGEIWQGRFLRTSGAEVVAELDLGTLLVAKFAGARLLDDIIYVKD
                     ALKQHLLGVLFNMVPESRLALVRDEYTRFLAKNSVESYGALVSSAMLSAVSVAEIVEA
                     LDGTYVCGERYSQRLVETYMIGAMSSEHALRYFQLTPNKVVIVGGDRAEIVLAALDTP
                     TVAVVLTGNYVPSPAVIERAEERGVPLVSVETDTVSAADGMRRLFGRLRVRERSKIEL
                     IARLIDESVDVDRLVRDLQA"
     gene            complement(6378..6560)
                     /gene="rpmF"
                     /locus_tag="VFZ86_14695"
     CDS             complement(6378..6560)
                     /gene="rpmF"
                     /locus_tag="VFZ86_14695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEX5643566.1"
                     /translation="MPVPKKKTSQRRRDQRRSQQKLSVETASVCPQCKSPKLPHRACP
                     KCGTYKGREVVVLEAD"
     gene            complement(6614..>6817)
                     /locus_tag="VFZ86_14700"
     CDS             complement(6614..>6817)
                     /locus_tag="VFZ86_14700"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEX5643567.1"
                     /translation="GESGATGEDEDEEAYVVAHQVDLERWVRDVIAEELPVKILCADD
                     CRGLCPVCGADLNADPGHVHEEA"
BASE COUNT          838 a         2509 c         2446 g         1024 t
ORIGIN      
        1 cgcgctccga gagctccgcg cactcggcct cggcgagcgg gttgaccggt ccgatgcgct
       61 cgcggcgacg cgacaggcgc tcgacgcgtt cccgtaggcc ggtctcctcg gcggcgtccg
      121 ccggcggcgc gaaccccgcc tggtcgatcc gctcgctcac ctcttcgagg ctggcggcga
      181 gctcggtgcg gcggtcctcg aggcgggcga gcgcgacctg gatggtcgtg cgccgctcgc
      241 cggcgttctc cagcccgcgg cgcagctgcg cctcgtcgtc ggccagcttg cgaagctccc
      301 cgcgctccag gccggcgccc tcctcatcgc ccacgtcgag ctgggcgatc aggcgcgtgg
      361 agtgcgcccg gagtgcgccg gtgcgcgcct ccaccgtcgc gcaggcggcg cgcacgtctg
      421 ggatgccggc gaggcgccgc tcgagctctt ccaggcgctc gcggtcggcg gcggcgcggc
      481 ggcgggcggc ctcgcgctgc tcggccacgc gcgcggcggc ggcgcggcgc tcctcgagct
      541 ccacgcgacg ccgggtcacg agggcgagtg cggccgaatg cgcggcctcg gaccggtgca
      601 gagccgcctc ggcctcttcc acggccgggc gcagctgctc ggactgcagc agcgcgttcg
      661 ccgccgtcgc gtccgcggcc tccttgctgg aggccgcctc ggccagctcg gcggcgatgc
      721 cctccacggc gcgccggtca cgctgcaggt ggtcctcgag gtcgcggcgg cgggagcgta
      781 cctgggccag cgcgcgctcc gccgtctccg ccgcgatgcg cgcctcgcgg tcggccgccg
      841 ccgtcgcgtc cccggcctcg cgcgccgcag tcaggcggct cacggccagc tgcacggcgg
      901 cctcctcgcg ggcctcccgc gccgacaccg cggcgagctt ctcgcccagc cgctccagtg
      961 aggcgcgacg ctcgagcaga agcgcggcgg ggactcctcc ggcaagcccg atctggccgt
     1021 cggccgggcg gtagtactcg cccgcccggg tgaccgccag acctgtgaac tcggccggca
     1081 ccgcacgcag gtcgtcgacg atgacgacgt cggccagagc gccctcgagg ccctggtcgc
     1141 cgaagctcac cttgtcggcg agcggggcgg cgccggggaa ggccgtggac ggcctcctgc
     1201 gcgccggcac gacgagcctg acgagaccga tgccggcgcg cttgagcgcg tcgagcagcg
     1261 accactggtc gacccccgcc ggcacggcca gcgcaccgga gagctgcgcg agcgctgcgg
     1321 ccagagcacg ctcgtacccc ggctcgcacg agacgctgcc ggcgagcgag accgtgcccg
     1381 ggaactcgcc ggccacctca aggacctcgc cgccgacgtc ctccatgtcg agcagcgcgg
     1441 cgcgcaggtg gtcggcctcg gcctccagcg cgcgccgctc catgagcgtc gcgcggtgcg
     1501 cgtcctccgc ggtcgtgacg tcgcgggccg cgtcggccac ggcggccgcc gccgcggccg
     1561 cggccgcctc ggtggtacgc agctgctcgc gggccgccgc ctccgcctcc gcggcctcgc
     1621 gctcctcacc ggccttgccg gtgacttccg cgctcatcgc ctcgtgctgc gccgcgaggc
     1681 gctcctgttc gccgccgagc gcctcaccgc ggcgctcgag ccgcgccgcc gtggacagcg
     1741 cgctctcgcg ttcgacggcg agccggtcga gcgcgacccg ccgctcggtc gacgcggccc
     1801 gcgcggcctc gagctcggcc gccgcgcccg cgtactcggt ctcggccgcc tcgagctcgc
     1861 ccgccaggcg gcgctcctcc tgcggccacg cgtcgtcgtc gcgctcctcc ggtcgcccgg
     1921 cgagctcggc gaccaggcgc tcgcgctccg ccgtcgccgc ccgctccacc tcgtccagca
     1981 ggtgccggcg ctgctcggcg aggcgccgca cgctctcgag ccgtccgtcg agcacgcgcg
     2041 cgcgcagcag acgacgagcg ctgcgctcgc gctccgccag cttgcgcgcg aacgcctcct
     2101 cctcgcgcgc ccgggcggcg gcgacgcggg cgagcccctg ctcgtgcgtc gcccgctccg
     2161 cgtcgatggc ggcgaggtcg tcgcgccgtg cctgcagatc cgcgtcgagc gcgagcaggt
     2221 cgccggtgag cagacgcccc ctggcctcgg cgatctcact ctccacggtg cgcagctgct
     2281 cggccgccgt cgcctgacgg cgcagcggcg ccagctgcga tcccacctcg cgttcgagga
     2341 gcagcgcccg ttcgagattg cggcgtacct cgcggagctt gagctccgcg cgctcacggc
     2401 gccgcttgta ggcgccaagg ccggcggcct cttcgatctg gctgcgccgg tcctccggct
     2461 tgccggccag gaacgactcc actttgccct ggccgatgat ggagtgcagt tccttgccga
     2521 gaccgacctg ggcgagcagt tcgacgacgt cggtgagacg gcacgcggac tggttgatgg
     2581 agtactgcga cgagccgtcg cgcgccacgc ggcgcatgac gctcacctcg gccgtgggca
     2641 gcggcagggc gccgtcggcg ttgtcgaagg tgagctccac ctcggcggcg ccgctcgcgc
     2701 ggcggccgtc gctgccggcg aagatgacgt cctgcatgga cgagccgcgc accgtggtgg
     2761 ggctctgctc gccgagcgcc cagatgaccg cgtcggcgag attgctcttg ccgctcccgt
     2821 tgggtccgat gatgacgccc acgccgggct cgaagaggag ctcggtctgt ttggggaacg
     2881 acttgaatcc cttgaggcgc agcgccttga ggtacacctc agccgacctc ggtggccccg
     2941 gcgggacccg ccgccgaggc gtcggcctcg tgcggcgcgg cctccccgac cggggcggcc
     3001 tccgcgaccg gaggggcctc ggggatgtct gcggcaccgg tctccgacgc ctgcgggcgt
     3061 cgcgagcggc tcccgcggag gcgagccttg cgcgcggtct tgcgggcgtg cgtctgcagc
     3121 tcgtgcagcg cctcggcggc ggcctcctgc tcggcgcgct tcttgctcgt cccgaacccg
     3181 cgccccaggg agtcgccgtc gacgaccgcc tcgatctcga actcccggtt gtgcgggggc
     3241 ccgctgaagc cgagcacctt gtaggcgacc gactgcccgc cgcgcgcgag aaactcctgc
     3301 agctccgtct tgtggtcgat gtagctgctc tccgcgaaga cgatgtgctc ctcgaacacc
     3361 tcgatgaccg ccggccgcac ggcctcgtag ccgaaggtga ggtagacggc gccgatgagc
     3421 gactccgtga ggtcggcgag cacgttctgg ttggtctgca gctgggcggc gtcgccgccc
     3481 tccccgccct ggccggcata ctggcgcagc agcttgccga ggccgagctt ggcggccacg
     3541 cgcgcgcagg tggcgcggct gacgatgtag gcccgcagcc gcgccaggtg cccctcggcg
     3601 aactccggga agcgccggta cagctcggtg gtgatgcaga gactcagcac gctgtcgccg
     3661 aggaactcga gccgctcgta cgagtgcacc cggtccggcg cccacgaggt gtgcgtgaac
     3721 acctggcgca gggtcgcctg gtccagctgc tgggcgagcg tgaggagtcg ttgcgtcaag
     3781 gtaggtgctc caacgacgac gaggggacgc ctcctgcctg caggagacac cccctcgtcg
     3841 gtccccctgg ggggatcggc gcggctaggc gtccacccgc gtcatgacga agtcgaccgc
     3901 gtcgccgacg gtcttgatgt cggcggcctc ttcgtcggtg atcttgatac cgaacttctc
     3961 ttccatggtc atgacgagct cgaccaggtc gagggaatcg gtctcgaggt cttcctggaa
     4021 acgggcatcc atggtgacct tttcttcgtc gacgctcagg gtctcgacga ggatcccctt
     4081 cacgtgctcg aacacctctt cacgggtcat gcagtgaacc tccgggcgag tttgggagag
     4141 tctacaccgc ggcggtctgc gtcccaagct cccgggcgat cgtgggcagc aggtcgctga
     4201 ccgcgccgcg ccgccccatg cggaccgcct gggcgatgcc tcgggccttg gagttgccgt
     4261 gggcgatcac caccggcgcg ttcatgccga cgagcagcgc ccctccgaac tcctcggggt
     4321 tcagcgcgtc cttcaggacc tggaggtcct tctgcagcag caggccgccc agcttggtct
     4381 tggcgctgac catgacggtg gccttgaggg cgccgaggat ggccctggcc gacccctcga
     4441 tcgtcttgag acccacattg ccggtgaagc cgtcggtgac gatcacgtcg acgagccggt
     4501 tcatgatgtc ccggccctcg atgttgccgt agaagtgcga gatgcggtgg tcgccggcga
     4561 tgagctggtg cgcctcgatc acgtcggggg tacccttggt cggctcctcg ccgatgctca
     4621 gcaggccgat ggtcggctcg gggatgccca tgacctcgcg cgcgaacgcc tggcccatga
     4681 tcgcgaactg gcggaggaac tccgcgcgga cctcggcgtt ggcgccgacg tccagaaacg
     4741 ccaccggcgc gggcatggcc ggcatgatgg tgcagattgc cggccgcttc acgccgggga
     4801 tgcggcgcac gtacagcacg ctcgccgcga ccaccgcgcc ggtgttgccg gcgctcacga
     4861 aagcttcggc ctcgccgttg tggaccatct tgaggccgac gacgatgctg gagccggtcc
     4921 tggcgcgggc cgcggtcgcc ggctcctcgt ggtagccgat cacgtccggg gcgtgcacga
     4981 cgctgacgtg ctgcacgccc tccggcagca gcgcgcgcac gcgcggctcg tcgccgacga
     5041 ggatgacgtt cagctggggg ccgcaggcgt cgagcgcgcc cttgacgatc tcgcccggcg
     5101 cgttgtcccc gccggccgcg tcgacgacca cgcggacgac acggtcgtcg gtgacggcgc
     5161 tccacgcgtc ggtcacccag tgcggcgacg tgtacggacg gttccttgag cccctcatgc
     5221 ctgcaggtcg cggaccagcc ggtcgacgtc gaccgactcg tcgatgaggc gggcgatgag
     5281 ctcgatcttg gagcgctccc tgacgcgcag gcggccgaac aggcggcgca tgccgtccgc
     5341 ggcggagacc gtgtcggtct cgaccgacac cagcggtacg ccgcgctcct cagcccgctc
     5401 gatcaccgcg gggctgggga cgtagttgcc ggtgagcacc acggcgacgg tgggcgtgtc
     5461 gagggcggcg agcacgatct cggctcggtc gccgccgacg atgaccactt tgttcggcgt
     5521 cagctggaag tagcggagcg cgtgctcgga gctcatcgcg ccgatcatgt aggtctcgac
     5581 caggcgctgg ctgtagcgct cgccacacac gtaggtgccg tccagcgcct cgacgatctc
     5641 ggccacggac acggcggaga gcatggcgct ggagaccagc gccccgtagc tctcgacgct
     5701 gttcttggcg aggaagcgcg tgtactcgtc gcgcaccagc gcgagacgag actcgggcac
     5761 catgttgaag aggacgccca ggaggtgctg cttgagggcg tccttgacgt agatgatgtc
     5821 gtcgagcagg cgcgcgccgg cgaacttggc cacgagcagg gtgccgaggt cgagctcggc
     5881 cacgacctcg gcgccgctgg tacgcagaaa gcgcccctgc cagatctcgc cgaggccctc
     5941 gcagacgacg atgtccctgc cggcacggat gcgctcgtag gcggccttga cgcggctcat
     6001 cgcgtcgacc tcggtgccgc tcagcacgtc gtgcagagcg tcctcgtcga ggacgaccgg
     6061 gcagatgtcg gtgaggtcgt cctcgagctc gagcacctct gcgacgaact cggcgtcctc
     6121 gtcgagcagg actccgtcgg cgtgcgccgc cagcgtgccc accggcttga agtacccggc
     6181 gtcgaggccg cgctcgcgca gggccatgca gaggccgacg gcgatggtgg tcttgccgcc
     6241 gctaccttgc gtagaagcga tgtagagcga tcgagtcacc gctcagcccc gccgggtcgg
     6301 tcagtccggc cgccgcgggg cccttcgggc cggcggccgc tcaatagggc tccgtgcact
     6361 gcgtggccct acgggcatta gtcagcttcg aggacgacga cctcgcggcc cttgtacgtg
     6421 ccgcacttgg ggcaggcgcg atgcgggagc ttggggctct tgcactgcgg gcagacgctc
     6481 gcggtctcca cgctgagctt ctgctgcgac cggcgctggt cgcggcgccg ctgggaggtc
     6541 ttcttcttgg gtacgggcat gacttcttct cctcgggtga atcagacaag ggactgtacc
     6601 atacggccgc gggtcacgct tcctcgtgca catgcccggg atccgcgttc aggtcggccc
     6661 cgcagacggg gcacagaccg cggcagtcgt cggcgcagag gatcttgacc ggcagctcct
     6721 cggcgatcac gtcacgcacc cagcgctcga ggtcgacctg gtgggcgacg acgtaggcct
     6781 cctcgtcctc gtcctcaccg gtggccccgg actcccc
//
LOCUS       DASUMX010000313         5321 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_123420, whole genome shotgun sequence.
ACCESSION   DASUMX010000313 DASUMX010000000
VERSION     DASUMX010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 5321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5321
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123420"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1146
                     /locus_tag="VFZ86_14705"
     CDS             <1..1146
                     /locus_tag="VFZ86_14705"
                     /inference="COORDINATES: protein motif:HMM:NF015922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HEX5643568.1"
                     /translation="EHAGLLARIKELRDLLGDESAVLGVIKGELLEIRRLYNDDRRTE
                     IVPDEGEIDLEDLIAEEQMAITITKTGYVKRLPVATYRQQKRGGIGVAGMDLKEEDEV
                     EHLFITSTHNFLLFFTSVGKVYRLKVHELPLAGRNAKGKHIVNLLPLRQDEKICAVIN
                     TRNFDESEGKYLILGTRRGIVKKTLFASYNTKLKADGIIAIKIREDDELLAVRHTSGD
                     DDVMMISRLGQAIRFHESDVRPTGRSASGVHGMRLRKGDEVISMDIARDDSDLFVITE
                     NGFGKRTPISEYRITGRGGLGVRTVALTDRKGYLVGVKVVRENHEIMLQSRDGVMIRV
                     RADGISRHGRATQGVRVMNMREGDVVSAIARMVVSESGATDEEIDAD"
     gene            1439..2125
                     /locus_tag="VFZ86_14710"
     CDS             1439..2125
                     /locus_tag="VFZ86_14710"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RibD family protein"
                     /protein_id="HEX5643569.1"
                     /translation="MLPHVVIHNEMSLDARMDGLDVDMGRFYTLAGTWHEDCTLVGSE
                     TLLAGMPELGGAAEPFALQPGEAGHSGGGASPDAPLLAAVDSRGRLPGLSQLRDQPYW
                     RDVIALCAGRTPAAHLDLLQADGVAAIVTGDERVDLRRALETLADEHDVKTVRVDAGG
                     ALIGALVRRGLVNEVSVVVEPRLVGGESHHWLMRAPDAGPGDVVALKLRELERLDDDA
                     LWLRYDVVPR"
     gene            complement(2162..2899)
                     /locus_tag="VFZ86_14715"
     CDS             complement(2162..2899)
                     /locus_tag="VFZ86_14715"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5643570.1"
                     /translation="MDNATGKRILALIREGDYAHPGEEAANELLFAGLRPDPRRRVLD
                     AGCGGAGTAAWVQERGLGAATGIEIDAATARLARERHPEVTVVRGDLQQAGAALSGPF
                     DLIYAMTAVYAAPDQAAVFRELGALAAPGAELRLLEYADPNGLFAAAAGRSASRGWWH
                     PLAPRHVPEILTAAGWTSVSVRDLHPEFVHWYHDLCLRIAAKRDEIARVFGDEWYAFV
                     AREYAGILGMIRAGELGGVLVRARRKP"
     gene            complement(2998..>5321)
                     /locus_tag="VFZ86_14720"
     CDS             complement(2998..>5321)
                     /locus_tag="VFZ86_14720"
                     /inference="COORDINATES: protein motif:HMM:NF012677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine cleavage system protein T"
                     /protein_id="HEX5643571.1"
                     /translation="RKDWGHFHEHLSHFPDVEMTDETGEIAMLALQGKTSRDILGGLI
                     ESGALPEPLRNRLSVATLRLGDDHPVEARIARTGYTGEPVAFELFVPAHDGPALWDTL
                     VGKGAVPVGLGARDTLRLEAGLPLYGHELGADPQGREIPIFSCPLAAFAVSFSPLKGD
                     FIGRQALERQQKAYAHILQRDYSHLEDLPRLTRPVAVTGRGIAREGAPVHADAADRFG
                     GESFLGWVTSGTAVPYWGVGGEGLCSLQTEEHELRSIALAYLDSRALEDDEVEVDVRG
                     RRVPGLVVPHHLRSDAPPCARAIIWDHVPQLTDLEGGEAGEKALRLLAKAIGNHEWRQ
                     HECIDLIPSEMTASPAARLLSISDPSGRYAEHKKAAAFYDADVFYYQGTSFIHEVERL
                     LVGEFRAYLGCPEVETRTVSGQMANTAVFSALVDYLNRADRKAEPRRIAKVLNNHIGK
                     GGHLSAQPMGALRDYVAHDPRTELPAVVDIPVLRDNPYKADVAALLDVIEQERPELII
                     LGKSMVLHPEPVYEVRQFLDSVGIPAVLMYDMAHVLGLAGPHFQEPFVEGADIVTGST
                     HKTFFGTQRGVVAARWVEADEKYDLWEAIERRTFPGSVSNHHLGTLLGLLMAAFEMNH
                     FKDAWQHAVIANAKAFALALHDAGLDVAGDPAVQYTETHQVVVRVGYGQGPEVAGRLE
                     DSGIIVNYQAAPDEEGFTAAGALRLGVAEMTRFGMGPAEFAEVARLMAAVVLGGRGVA
                     AEVRALREPFTEMGYCFKGPEFEPLLRRLHDLI"
BASE COUNT          823 a         1810 c         1916 g          772 t
ORIGIN      
        1 gagcacgccg gcctcctggc gcgcatcaag gaactgcgcg atctgctcgg cgacgagagc
       61 gccgtcctcg gcgtcatcaa gggcgagttg ctcgagatcc ggcgcctcta caacgacgac
      121 cggcgcaccg agatcgtccc cgacgagggc gagatcgacc tcgaggacct catcgccgaa
      181 gagcagatgg cgatcaccat caccaagacc ggctacgtca agcggctgcc cgtcgccacg
      241 taccgccagc agaagcgcgg cggcatcggt gtcgccggta tggacctcaa ggaagaggac
      301 gaggtcgagc acctgttcat cacgagcacc cacaacttcc tgctcttctt cacgagtgtg
      361 ggcaaggtgt accggctcaa agtgcacgag cttccgctgg ccgggcgcaa cgccaagggc
      421 aagcacatcg tcaacctgct gccgctgcgc caggacgaga agatctgcgc cgtcatcaac
      481 acgcgcaact tcgacgagag cgagggcaag tacctcatcc tcggcacgcg caggggcata
      541 gtgaagaaga cgctgttcgc ctcctacaac accaagctca aggccgacgg catcatcgcc
      601 atcaagatcc gcgaggacga cgagctgctg gccgtgcgcc acacctcggg tgacgacgac
      661 gtcatgatga tcagccggct cggccaggcc atccgcttcc acgagagcga cgtccgcccc
      721 accggccgca gcgcctcggg agtccacggc atgcgtctgc gcaagggcga cgaagtgatc
      781 agcatggaca tcgcccgcga cgacagcgac ctcttcgtga tcacggagaa cggattcggc
      841 aaacgaacgc cgatcagcga gtatcgcatc accggccgcg gcggcctcgg cgtgcggacg
      901 gtcgcgctga ccgaccgcaa gggctacctc gtcggcgtca aagtcgtgcg cgagaaccac
      961 gagatcatgc tgcagagccg cgacggcgtc atgatccgcg tgcgcgccga cggcatcagc
     1021 cggcacggac gggccacgca gggcgtcagg gtgatgaaca tgcgcgaggg tgacgtcgtg
     1081 agcgccatcg cccgtatggt cgtcagcgag agcggcgcga ccgacgagga gatcgacgcc
     1141 gattgaccga cgaagcgggg tcaacgtggg gtgcggcgta gcggcacctc acactagcgc
     1201 ccgcaggcgg gctaacgtgc ggggcgccgt gaggcacacc gcacgatgac cgacgaagcg
     1261 gggtcaacgt tcgagggagc cgggagaggg ccggggaggc gatgcctccc cggccctctc
     1321 tctgtctctg ggcgccttcc tcatgcaccg aacggcaccc cggtgtgcgc ccttcccgcg
     1381 tccggtcggt gggatagagt ccggaccact cgacatctca cgcgcaagga gcgacagcat
     1441 gctgccacac gtcgtcatcc acaacgagat gagtctggat gcccgcatgg acggcctcga
     1501 tgtcgacatg ggcaggttct acacactggc ggggacgtgg cacgaggact gcaccctcgt
     1561 gggcagcgag acgttgctgg ccggcatgcc ggagttgggc ggcgcggccg agcctttcgc
     1621 actgcaaccc ggtgaagccg ggcacagcgg tggaggagca tcgccggacg cacccctgct
     1681 ggccgcggtc gactcgcgcg gccggctgcc cgggctgagc cagctgcgcg accagcccta
     1741 ctggagggat gtgatcgcgc tctgcgcggg gcggacgccg gctgcgcatc tcgacctgct
     1801 gcaggccgac ggcgttgccg cgatcgtcac tggggacgag cgcgtcgacc tgcgccgcgc
     1861 tctcgagacg ctcgcggacg agcacgacgt caagacggtg cgcgtggacg ccggcggggc
     1921 gctcatcggg gcgttggtgc ggcgcgggct cgtcaacgag gtcagcgtgg tcgtcgagcc
     1981 ccgactcgtc gggggcgaga gccaccactg gctcatgagg gctcccgacg ccggtcccgg
     2041 cgacgtggtg gctctcaagc ttcgcgagct tgagcggctg gacgacgacg cgctctggct
     2101 gcggtacgac gtcgtgccgc ggtagcctcg ccggccggcc ggagagctac ggagtcgggc
     2161 gtcagggctt gcgccgcgcg cggacgagca cgccgccgag ctcgccggca cggatcatcc
     2221 caaggatgcc ggcgtactcg cgggcgacga acgcgtacca ctcgtcgcca aagacccgcg
     2281 cgatctcgtc gcgcttcgcg gcgatgcgca ggcacaggtc gtggtaccag tgcacgaact
     2341 ccggatggag gtcgcgcacc gagaccgagg tccagccggc ggccgtgagg atctccggca
     2401 cgtggcgcgg ggcgagcgga tgccaccagc cacggctggc gctcctgccg gcggcagcgg
     2461 cgaagaggcc gttggggtcg gcgtactcca gcaggcgcag ctcggccccg ggtgcagcca
     2521 gcgcgccgag ctcgcggaac acggccgcct ggtcgggcgc tgcgtagacc gcggtcatgg
     2581 cgtagatgag gtcgaagggg ccgctcagcg ccgcgccggc ttgctgcagg tcgccccgga
     2641 ctaccgtcac ctccggatgt cgctcgcgcg cgaggcgggc ggtcgcggcg tcgatctcga
     2701 tgccggtcgc cgccccaagc ccacgctcct gcacccaggc ggccgtgccg gcgccgccgc
     2761 agccggcgtc caggacgcgg cggcgcgggt cgggtcgcaa cccggcgaag agcagctcgt
     2821 tggcggcctc ttcgccgggg tgcgcgtaat cgccctcccg gatcagcgcc aggatgcgct
     2881 tgcccgtggc gttgtccatc gccgtcggac ggtcgggcgc gccgtcctgc tcgccgggtg
     2941 gccgtcctga cggccgatct ggggccacgc ctgggttcct catccgggct ccgtcgctca
     3001 gatcagatcg tgcagtcgcc gcagcagcgg ttcgaactcc ggacccttga agcagtagcc
     3061 catctccgtg aagggctcgc gcagggcgcg gacctcggcg gcgacgccgc ggccccccag
     3121 caccaccgct gccatcaggc gggcgacctc ggcgaactcc gccggaccca tgccgaagcg
     3181 cgtcatctcg gccacgccga ggcgcagggc gcccgcggcg gtgaatccct cctcgtcggg
     3241 cgctgcctga tagttgacga tgatgccgct gtcctcgagc cggccggcga cctcgggacc
     3301 ttgcccgtac ccgacccgca ccacgacctg gtgcgtctcg gtgtactgca cggcggggtc
     3361 gccggcgacg tcgagaccgg cgtcgtgcag cgccagggcg aacgccttgg cgttggcgat
     3421 cacggcatgc tgccaggcgt ccttgaagtg gttcatctcg aacgcggcca tgagcaggcc
     3481 gagcagggtc ccgaggtggt ggttgctgac cgagccgggg aacgtgcgcc gctcgatcgc
     3541 ctcccagagg tcgtacttct cgtcggcctc gacccagcgc gccgcgacga cgccgcgctg
     3601 cgtgccgaag aacgtcttgt gcgtcgagcc ggtgacgatg tcggcgccct ccacgaacgg
     3661 ctcctggaag tgagggccgg cgaggccgag cacgtgggcc atgtcgtaca tcagcacggc
     3721 cgggatgccg accgagtcca ggaactggcg cacctcgtac acgggctcgg ggtgcagcac
     3781 catgctcttg ccgaggatga tgagttcggg gcgctcctgt tcgatcacgt cgaggagcgc
     3841 cgctacgtcc gccttgtagg ggttgtcgcg caggacgggg atatcgacga ccgccggcag
     3901 ctcggtgcgc gggtcgtggg ccacgtagtc gcgcagcgcc cccatgggtt gcgcgctcag
     3961 gtggccgcct ttgccgatgt ggttgttgag gaccttggcg atgcgtcgcg gctcggcctt
     4021 gcggtcggcg cggttgaggt agtcgaccag ggcactgaag accgccgtgt tggccatctg
     4081 gccgctgacc gtgcgtgtct cgacctccgg acagccgagg taggcgcgga actcacccac
     4141 cagcagccgc tccacctcgt ggatgaagct cgtgccttga tagtagaaga cgtcggcgtc
     4201 gtagaaggcc gcggctttct tgtgctccgc gtagcggccc gacggatcgg agatgctgag
     4261 caggcgtgcc gccggtgagg cggtcatctc gctggggatg aggtcgatgc actcgtgctg
     4321 acgccactcg tgattgccga tcgccttggc gaggaggcgc agcgccttct cgccggcctc
     4381 cccgccctcg aggtcggtga gctgcgggac gtggtcccag atgatcgcgc gggcgcacgg
     4441 cggtgcgtcg ctgcgcaggt ggtgaggcac cacgagcccg gggacgcgac ggccgcgcac
     4501 gtcgacctcg acctcgtcgt cctcgagcgc gcggctgtcg aggtaggcga gagcgatgga
     4561 gcgcagttcg tgctcttctg tctgcagcga gcagaggcct tcgccgccga cgccccagta
     4621 ggggacggcg gtgccgctgg tgacccagcc gaggaagctc tcgccgccga agcggtcggc
     4681 ggcgtcggcg tggacggggg cgccctcgcg ggcgatgccg cggccggtca cggcgaccgg
     4741 ccgcgtgagc cgcggcagat cctccaggtg cgagtagtcg cgctgcagga tgtgcgcgta
     4801 ggccttctgc tggcgctcga gcgcttgccg gccgatgaag tcgcccttca atggcgagaa
     4861 gctgacggcg aacgctgcca gcggacagct gaagatgggg atctcacggc cttgtgggtc
     4921 cgcgcccagc tcgtggccgt agagaggcag gccggcctca agccgcaggg tgtcgcgggc
     4981 gccgagcccg acgggcacgg cgcccttccc gaccagcgtg tcccagaggg ccggcccgtc
     5041 gtgcgccggc acgaagagct cgaaggcgac cggttcgccg gtgtagccag tgcgggcgat
     5101 gcgcgcttcc acggggtggt cgtcacccag acgcagtgtc gccacgctca gcctgttgcg
     5161 caacggctcc ggcagcgccc cactctcgat caggccaccc aggatgtccc gggacgtctt
     5221 gccctgcagg gccagcatcg cgatctcgcc ggtctcgtcg gtcatctcga cgtcggggaa
     5281 atgcgagaga tgctcgtgga agtgccccca gtccttgcgt c
//
LOCUS       DASUMX010000314         7443 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1416880, whole genome shotgun sequence.
ACCESSION   DASUMX010000314 DASUMX010000000
VERSION     DASUMX010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 7443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7443
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1416880"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="VFZ86_14725"
     CDS             <1..309
                     /locus_tag="VFZ86_14725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013628376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl--tRNA ligase-related protein"
                     /protein_id="HEX5643572.1"
                     /translation="NIAAGDLGAPAAQKFDCEAWLPGQQRYRELTSCSNCTDYQARRL
                     NTRFRTEKGPRFVHTLNGTGVAVGRTLIAIMENYQQADGSITVPEVLRAALGKDVIGA
                     "
     gene            complement(334..1236)
                     /locus_tag="VFZ86_14730"
     CDS             complement(334..1236)
                     /locus_tag="VFZ86_14730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX5643573.1"
                     /translation="MATVAGHAATPLIEPVDRREGVGHSIVRALSHAPVTILLVVIGV
                     AWLVPTLGLFLTSLLPAQLISSEGWWNVLVTPSEATLENYRDIFADEAITSALLTTVW
                     ISVGSTILPIVVAALAAYAFAWMDFPGRDWLFVGVIAMIVVPIQMALIPIFSLYDRVH
                     LFDTIPGLILFHTAFGLPLAIFLLRNFFVGIPAELLEAARIDGASELRIFTRLILPLS
                     WPAIASLAIFQFLWTWNDLIVALSLARSTQPITVAIFTQLRQFGANIDVIAPAAFLSL
                     AIPLVVFFAFQRYFVQGLLAGSVK"
     gene            complement(1236..2591)
                     /locus_tag="VFZ86_14735"
     CDS             complement(1236..2591)
                     /locus_tag="VFZ86_14735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017547027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX5643574.1"
                     /translation="MATGSITAEPPERGAPPAAADGGADPGRGRRYLVAFAFLAPALA
                     FLGVWVVYPTINTFVRSFYDRSGDSFIGFDNYVTLFTTDVLLTAIRNNAIWVLVVPAT
                     VTAIGLVFAVLTERIRWSVAFKTAVFMPMAISLFAAGVIWHIMYEKDPSQGTVNAAIS
                     VVSDAVNPPGVLAGARPSGDDLQGSAARGYTLQGALQPGQVAQLGLTGIRASDVADGV
                     QAVAPAPAEGGITGVVWRDFKPGGGGTAGEVEEGEVGVPGVTLRLTDDGGAEVATATS
                     GADGVFAFEGLSQGGYTVGISSQTFRQPFAGVPWLGPRLITPAIIIAYIWVWAGFAMV
                     VIAAGLAAIPRDVLEAARTDGATEWQVFRRVTVPLLAPVLSVVFITMIINVLKVFDII
                     LSVAPASVQDDVNVIALAMWRTSFGGVNDFGLGSAIAAFLFLLVIPVLLLNVRRFRRE
                     A"
     gene            complement(2598..3935)
                     /locus_tag="VFZ86_14740"
     CDS             complement(2598..3935)
                     /locus_tag="VFZ86_14740"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX5643575.1"
                     /translation="MSRLTWIIIAVVVAIAVIVGVVVVANDGGKITESGAGEPSETPA
                     ADVSGTVSVMAVWSGDEQASFQAVIDGFTELYPNVSVEYTGAGDQLPTVLGTAVQGGN
                     PPSVAAVAQPGLMRDFVSQGALKPLDFASDVINENFSSSTIEVGTVDGTLYGLLFKGA
                     NKSTVWYNVPAFEDAGVTPPATWDELLQSAETLNASGVTAYSIAGADGWTLTDLFENI
                     YLRTAGPDLYDQLTAHEIPWTDASVTTALEAMAQVFGDTGNIAGGTSGALQTDFPKSV
                     TQVYTDPPAAAMVFEGDFVQGEILNSTQAEPETGFNQFAFPAVDDSPPSVVGGGDMII
                     AFDDDPATQAFVAYLATPEASEIWASRGGFSSPNVNVDTSVYPDAIAQATAAALADAE
                     VFRFDMSDLAPSAFGGTVGQGMWGILQDFLQDPGDVQGTQQRLENAAARAYGQ"
     gene            complement(3961..5226)
                     /gene="ugpC"
                     /locus_tag="VFZ86_14745"
     CDS             complement(3961..5226)
                     /gene="ugpC"
                     /locus_tag="VFZ86_14745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020553318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEX5643576.1"
                     /translation="MAVVAFRGVSKVYAGGARAVSHMDLEIGDGEFVVLVGPSGCGKT
                     TALRMLAGLETITEGAITIGDRVVNHVAPKDRDIAMVFQNYALYPHLTVYDNIAFGLR
                     LRKTPKDEIDERVRHAAEVLGLEDFLERKPRALSGGQRQRVAMGRAIVREPQAFLMDE
                     PLSNLDAKLRVQMRAEISSLQDELGVTTVYVTHDQIEAMTMGDRVAVMRKGELQQVAP
                     PQELYDRPVNLFVAGFIGSPTMNMMEAVVESDDGGGLALTIGEQRLRLAPETRRAHPA
                     LERYIGRGVVLGVRPEDLEDAALAGDAPEDRRLRGRLMLREALGSEVVAHFEIDAPPV
                     LTEDTRELAEDIGTGHHYEAHAKRRTTTSKLVGRFSARTAVREGETLEIAVDTRALHF
                     FDVESGLGIYGGEEARTRPVPEQPRPVAA"
     gene            complement(5339..6409)
                     /locus_tag="VFZ86_14750"
     CDS             complement(5339..6409)
                     /locus_tag="VFZ86_14750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643577.1"
                     /translation="MTTTPPPDGGVHDGLPSSGVRWGRFVAGALLAAALLVGAVAAFN
                     LLTDPWGVFGVGIFPPRVNQDRSTKADLLTGLKRPPELLIYGSSRAWTVEAARVEQAT
                     GLRTFNAAVTAGRPADAYVFTAVVHDTWPRAEPDFLWLLDVEAFQRGPLPPSLLAESR
                     FSRYLPWRAKAAAQLDELGWLASWSGLQASWEVWKKRPTWEKVRASWLKRIAPDGTVK
                     TQPSSEAGAGPKALARWSEDEVARYRSFARLDADAETYVEKTLQLFASWGGSGVVVLT
                     PTQPQVLAAVRDAGGRRRHAEVLRLLGRLQEQYDFAVVDLTGVESFGGDPSGFFDATH
                     MTTENQRRMIDAALARADERFD"
     gene            complement(6447..6857)
                     /locus_tag="VFZ86_14755"
     CDS             complement(6447..6857)
                     /locus_tag="VFZ86_14755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643578.1"
                     /translation="MNALIIGCSDGRVAAQLDALLVQLGAPDANRLIVPGGVLPLTRP
                     GSERRVALDCVREIVEVHDVRTIFLVAHQECAAYERALGGLGFDQQELLERDLRRVKT
                     LLETTLPGVEVRCYVIPWRENGEGAAYGPAALVD"
     gene            6944..7033
                     /locus_tag="VFZ86_14760"
     tRNA            6944..7033
                     /locus_tag="VFZ86_14760"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6978..6980,aa:Ser,seq:tga)
     gene            complement(7234..>7443)
                     /locus_tag="VFZ86_14765"
     CDS             complement(7234..>7443)
                     /locus_tag="VFZ86_14765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012945931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 family protein"
                     /protein_id="HEX5643579.1"
                     /translation="RAFPDMTGARDDQTVWFSFIVFESKEHRDEVNAKVMKEMGEKAE
                     EYKDMVMPFDMRQMAYGGFQVEVEG"
BASE COUNT         1172 a         2600 c         2581 g         1090 t
ORIGIN      
        1 aacatcgccg ccggcgacct cggcgccccg gcggcgcaga agttcgactg cgaggcgtgg
       61 ctcccgggcc agcagcgcta ccgggagctc accagctgca gcaactgcac cgactaccag
      121 gcgcggcggc tcaacacccg cttccgcacg gagaaggggc cgcgcttcgt gcacacgctc
      181 aacggcaccg gcgtggccgt cggccgcacc ctcatcgcca tcatggagaa ctaccagcag
      241 gccgacggca gcatcacggt gccggaggtg ctgcgggcgg cgctgggcaa ggacgtcatc
      301 ggcgcctgag taccttctgg tccctcgcgc gggctacttg accgacccgg cgagcagccc
      361 ctgcacgaag taccgctgga aggcgaagaa caccaccagc gggatcgcca gcgagaggaa
      421 cgcagccgga gcgatcacgt cgatgttggc gccgaactgg cggagctgcg tgaagatcgc
      481 cacggtgatg ggctgcgtgc tgcgcgccag cgacagggcg acgatgaggt cgttccacgt
      541 ccacaggaac tggaagatgg cgagcgaggc gatcgccggc cagctcaggg gcaggatcag
      601 ccgggtgaag atccgcagct ccgaggcgcc gtcgatgcgc gccgcctcca gcagctccgc
      661 cggaatgccc acgaagaagt tccggaggag gaagatcgcc agcggcaggc cgaacgccgt
      721 gtggaacagg atgaggcccg ggatcgtgtc gaacaggtgg acgcggtcgt acagcgagaa
      781 gatcgggatg agcgccatct ggatgggcac cacgatcatc gcgatcacac cgacgaacag
      841 ccagtccctg ccggggaagt ccatccaggc gaacgcgtag gcggccaggg ccgcgacgac
      901 gatgggcagg atcgtcgagc ccacggagat ccacaccgtg gtgagcagcg cactcgtgat
      961 cgcctcgtcg gcgaagatgt cacggtagtt ctccagcgtg gcctcgctcg gcgtgacgag
     1021 cacgttccac cagccctcgg acgagatgag ctgagcgggc agcagcgacg tcaggaacag
     1081 gcccagcgtc ggcaccagcc acgcgacgcc gatgacgacg agcaggatcg tgaccggcgc
     1141 gtggctcagg gcacgcacga tgctgtgccc gacgccttca cggcggtcga cgggctcgat
     1201 cagcggcgtt gccgcatgac ctgcgacggt ggccatcatg cctcccttct gaagcggcgc
     1261 acgttgagca gcagcaccgg gatgacgagc aggaacagga acgcggcgat cgcggagccc
     1321 agcccgaagt cgttgacgcc gccgaacgac gtgcgccaca tcgcaagggc gatgacgttg
     1381 acgtcgtcct gcaccgacgc cggggcgacg gacaggatga tgtcgaacac cttgagcacg
     1441 ttgatgatca tggtgatgaa caccacgctg agcaccggcg ccagcagcgg caccgtcacg
     1501 cgccggaaga cctgccactc ggtggctccg tccgtcctcg ccgcctccag cacgtcgcgg
     1561 gggatggcgg cgagccccgc ggcgatcacg accatcgcga agcccgccca cacccagatg
     1621 taggcgatga tgatggcggg tgtgatgaga cgcggcccga gccagggcac gccggcgaag
     1681 ggctgccgga aggtctggct cgagatgccg acggtgtagc cgccctggga gaggccctcg
     1741 aacgcaaaga cgccgtcggc tccgctcgtg gcagtcgcga cctccgctcc accgtcgtcc
     1801 gtcagtcgca gggtgacccc gggaacgccc acctcgccct cctcgacctc gccggctgtg
     1861 cctcctccgc ccggcttgaa gtctcgccac accacgccgg tgatgccgcc ctccgccggc
     1921 gcgggagcca ccgcctgcac accgtcggcc acgtcgctcg cccggatgcc ggtgagcccg
     1981 agctgggcca cctgcccggg ctggagcgcg ccctgcagcg tgtagcctcg cgccgcggac
     2041 ccttgcaggt cgtcgcccga cggcctcgct ccggcgagca cgccgggcgg gttcaccgcg
     2101 tccgacacca ccgagatggc cgcgttgacg gtgccctggc tgggatcctt ctcgtacatg
     2161 atgtgccaga tgacgccggc cgcgaacagg gagatcgcca tcggcatgaa gaccgcggtc
     2221 ttgaacgcca ccgaccagcg gatgcgctcg gtgagcaccg cgaacaccag gccgatggcg
     2281 gtcaccgtcg ccggcaccac gaggacccag atcgcgttgt tgcggatcgc cgtgagcagg
     2341 acgtcggtcg tgaacagcgt gacgtagttg tcgaacccga tgaacgagtc accggaccgg
     2401 tcgtagaagc tgcggacgaa cgtgttgatc gtcgggtaca cgacccagac gcccaggaac
     2461 gcgagcgccg gggcgagaaa ggcgaacgct acgaggtacc ggcgcccgcg gcccggatcg
     2521 gctccgccgt cagcggcggc gggcggagcc ccccgctcgg ggggctccgc cgtgatgctg
     2581 ccggtcgcca tggccgatca ctgtccgtag gccctcgccg cggcgttctc gagccgctgc
     2641 tgtgtgccct ggacgtcgcc gggatcctgg aggaagtcct gcaggatccc ccacatgccc
     2701 tgaccgaccg tgccgccgaa cgccgacggc gccaggtcgg acatgtcgaa gcggaagacc
     2761 tcggcgtccg ccagtgcagc cgccgtcgcc tgggcgatcg cgtccggata gacgctcgtg
     2821 tcgacgttga cgttgggcga cgagaacccg ccgcgggacg cccagatctc cgacgcctcg
     2881 ggcgtggcca ggtacgcgac gaacgcctgc gtcgccgggt cgtcgtcgaa ggcgatgatc
     2941 atgtcgcccc cgcccacgac gctgggcggc gagtcgtcga ccgccgggaa ggcgaactgg
     3001 ttgaagcccg tctcgggctc cgcctgggtc gagttcagga tctcgccctg cacgaagtca
     3061 ccctcgaaca ccatggccgc cgcgggcggg tcggtgtaga cctgcgtcac cgacttgggg
     3121 aagtcggtct gcagggcgcc ggacgtgccg ccggcgatgt tgccggtgtc gccgaagacc
     3181 tgcgccatcg cctccagggc ggtggtcacc gaggcatcgg tccaggggat ctcgtgcgca
     3241 gtcagctggt cgtacaggtc ggggccggcc gtgcgcaggt agatgttctc gaacaggtcg
     3301 gtgagcgtcc agccgtccgc gccggcgatg gagtaggccg tcacgccgga ggcgttcagg
     3361 gtctcggcgg actgcagcag ttcgtcccac gtcgccggcg gggtcacgcc ggcgtcctcg
     3421 aacgccggca cgttgtacca gaccgtggac ttgttggcgc ccttgaacag caggccgtac
     3481 agcgtgccgt ccacggtccc gacctcgatg gtgctgctcg agaagttctc attgatcacg
     3541 tccgacgcga agtccagagg cttgagggcc ccctgactca cgaagtcgcg catgagcccg
     3601 ggctgtgcga ccgcagcgac gctcggcggg tttccgccct gcacggcggt gccgagcacc
     3661 gtcgggagct ggtcgccggc ccccgtgtac tcgacgctca cgttgggata gagctcggtg
     3721 aagccgtcga tcacggcctg gaacgaggcc tgctcgtcgc cggaccagac ggccatcacg
     3781 gagacggtcc cgctcacgtc ggcggcgggc gtctcgctcg gctcacccgc gccggactcg
     3841 gtgatcttgc cgccgtcgtt cgcgacgacg acgacgccta cgatgacagc gatcgctact
     3901 acgaccgcga tgatgatcca tgtcagtctg ctcatctgtg acaactcctt cagtactccg
     3961 tcaggcggcg accgggcgcg gctgctccgg aacgggccgg gtccgcgcct cctcgccgcc
     4021 gtagatgccg aggccggact ccacgtcgaa gaagtggagg gcacgggtgt cgacggcgat
     4081 ctccaaggtc tcgccctcgc gcaccgcggt gcgcgcgctg aaccggccga cgagcttgga
     4141 ggtcgtcgtc cggcgtttgg cgtgcgcctc gtagtgatga cccgtgccga tgtcctcggc
     4201 gagctccctg gtgtcctcgg tgaggacggg gggagcgtcg atctcgaagt gcgcgacgac
     4261 ctcggagccg agcgcctcgc gcagcatcaa ccgcccgcgg aggcgccggt cctcgggcgc
     4321 gtccccggcg agcgccgcgt cctccaggtc ctccggtcgc acgccgagca cgacgccgcg
     4381 accgatgtac cgctcgagtg cggggtgcgc gcggcgcgtc tccggcgcca gtctcaggcg
     4441 ctgctcgccg atcgtcaggg cgagccctcc gccgtcgtcc gactcgacga ccgcctccat
     4501 catgttcatg gtggggctgc cgatgaaccc ggcgacgaac aggttgaccg ggcggtcgta
     4561 cagctcctgc ggcggggcga cctgctggag ctcgcccttt ctcatgaccg cgacgcggtc
     4621 gcccatggtc atcgcctcga tctggtcgtg cgtcacgtag accgtggtca cgcccagttc
     4681 gtcctgcagg ctgctgatct ccgcgcgcat ctgcacgcgg agcttggcgt ccaggttgga
     4741 gagcggctcg tccatgagga acgcctgcgg ctcgcggacg atcgcgcgcc ccatggcgac
     4801 ccgctggcgc tggccgccgg agagggcgcg cggcttgcgc tcgagaaagt cctcgagccc
     4861 gagcacctcc gcggcatgcc gtactcgttc gtcgatctcg tccttcggtg tcttgcgcag
     4921 gcgcaagccg aaggcgatgt tgtcgtacac ggtgaggtgc gggtacagcg cgtagttctg
     4981 gaacaccatg gcgatgtccc ggtctttggg ggccacgtgg ttcaccacac ggtcgccgat
     5041 ggtgatggcg ccttcggtga tggtctcgag gccggcgagc atgcgaaggg ccgtcgtctt
     5101 gccacagccc gacggcccga ccaggacgac gaactcgccg tcaccgatct cgaggtccat
     5161 gtgcgatacg gcgcgggctc cgcccgcgta gaccttgctg accccccgga aagctacgac
     5221 agccatcggt gcctcccgga tcgaccgaaa ccccccgggg gtattcggcc cttgtgccgg
     5281 tacggttccc cggccgcgac ggacctaacg gggtaccgta gccggagcgc cgcggcggtc
     5341 agtcgaagcg ctcgtcggcg cgcgcgagcg cggcgtcgat catcctgcgc tggttctccg
     5401 tggtcatgtg ggtggcgtcg aagaacccgg agggatcgcc tccgaacgac tcgacgccgg
     5461 tcaggtcgac gacggcgaag tcgtactgct cctgcagcct gccgaggagg cgcaggacct
     5521 cggcgtggcg cctccgcccg cccgcgtccc gcaccgccgc cagcacctgc ggctgcgtcg
     5581 gcgtgagcac gacgacccca ctcccgcccc aggaggcgaa cagctgcagc gtcttctcga
     5641 cgtaggtctc ggcgtcggcg tcgagacgtg cgaaggaccg gtaccgcgcg acttcgtcct
     5701 cgctccagcg ggcgagcgcc ttcggcccgg cgccggcctc actcgagggc tgcgtcttga
     5761 ccgtgccgtc gggcgcgatc cgcttgagcc agctcgcccg caccttctcc cacgtcgggc
     5821 gcttcttcca cacctcccag gacgcctgca gaccgctcca cgacgcgagc cagcccagct
     5881 cgtcgagctg ggccgccgcc ttggcccgcc aaggaaggta gcggctgaac cgcgattccg
     5941 cgagcagcga cggcggcagc gggccgcgct ggaacgcctc cacgtcgagc agccagagga
     6001 agtccggctc cgcccgcggc caggtgtcgt gcacgaccgc cgtgaacacg taggcgtccg
     6061 ccggccggcc ggcggtgacg gcggcgttga aggtccgcag gccggtggcc tgctccaccc
     6121 gcgccgcctc caccgtccac gcgcgcgagg agccgtagat gagcagctcc ggcggacgct
     6181 tcagccccgt gagcaggtcc gccttcgtgg agcggtcctg gttgacgcgc ggcgggaaga
     6241 tgccgacgcc gaacacgccc cacgggtcgg tgagcaggtt gaaggcggcg acagcgccca
     6301 ccagcagcgc ggccgcgagc agcgccccgg ccacgaagcg gccccagcgg acgccggacg
     6361 acggcagacc gtcgtgcacg ccgccgtctg gaggaggggt cgtcgtcatc gcgagagggt
     6421 cgctgggccc gccccgggga cgaaggtcag tcgaccaggg cggcgggccc gtacgcggcg
     6481 ccctcgccgt tctcgcgcca ggggatcacg tagcagcgca cctcgacgcc ggggagcgtg
     6541 gtctcgagca gcgtcttgac ccgccgcagg tcgcgctcga gcagttcctg ctggtcgaaa
     6601 ccgagaccgc cgagcgcccg ctcgtaggcg gcgcactcct ggtgcgcgac caggaagatg
     6661 gtgcgcacgt cgtgcacctc gacgatctcg cgcacgcagt cgagcgcgac gcgtcgctcc
     6721 gagcccggac gcgtgagcgg cagcacccct ccgggcacga tgagccggtt ggcgtccggg
     6781 gcgccgagct ggacgagcag ggcgtcgagc tgggcggcga cgcggccgtc cgagcagccg
     6841 atgatgagcg cgttcatggg cgcgagtatc tcagacgcgg ccgcgggcgg ccagccgcgc
     6901 gatgccgagc ggccggccaa gcacttggta cacttgcctt accggagggg tggccgagag
     6961 gtcgaaggca gcggtcttga aaaccgccaa cggggcgact cgttcgtggg ttcgaatccc
     7021 accccctccg ccaatttgca ggaaagagcg tccgcggcga accctctccc ccaccttccc
     7081 gcccggttcg tgcgccatcg tgcgccaaga tcacgggcaa taagtgctga ccgtggtcgt
     7141 caagactcgt tcggaggtgg cgccgacagc gcccgcggcc gcgggtctcg gacctgaatg
     7201 gtcgctttcc ggctcttcct tcgttttcga tgcctatcct tcgacttcga cctggaagcc
     7261 tccataggcc atttgcctca tgtcgaaggg catgaccata tccttgtatt cttccgcctt
     7321 ctcccccatc tccttcatca ccttggcatt cacctcatcg cgatgctctt tggattcgaa
     7381 gacgatgaag gagaaccata cggtctggtc gtcccgggct ccggtcatat caggaaatgc
     7441 gcg
//
LOCUS       DASUMX010000315         4881 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1993089, whole genome shotgun sequence.
ACCESSION   DASUMX010000315 DASUMX010000000
VERSION     DASUMX010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4881
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1993089"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="VFZ86_14770"
     CDS             <1..180
                     /locus_tag="VFZ86_14770"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012489100.1"
                     /note="catalyzes the phosphorylation of
                     N-acetyl-L-glutamate to form N-acetyl-L-glutamate
                     5-phosphate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEX5643580.1"
                     /translation="LGALQAAGRIGGGMVPKAAAVRKALEAGVGSAHIIDGRVEHAVL
                     LEIMTDAGCGTKVVA"
     gene            270..728
                     /locus_tag="VFZ86_14775"
     CDS             270..728
                     /locus_tag="VFZ86_14775"
                     /inference="COORDINATES: protein motif:HMM:NF013483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEX5643581.1"
                     /translation="MSKTERQRLIQTVVQQREIATQRELVSALSALGCEVTQATVSRD
                     IRELDLQKVRTHLGRAKYVLSSRERPKDPEQAARHVLRDFARSTAVSQQLLVVRCEIG
                     TASTVARQIDNLNHTDIVGTLAGDDTFVVILDTSDKAREMKRYVDRLREA"
     gene            840..2117
                     /locus_tag="VFZ86_14780"
     CDS             840..2117
                     /locus_tag="VFZ86_14780"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010899330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEX5643582.1"
                     /translation="MTTPSSGNGSAPNGTVVLAYSGGLDTSALVPWLKDKYGYDVVCC
                     LVDIGRVKDVPGIMARAEAAGAIAAVAIDAKEEFAEHHCLPALMANALYEDTYPLSTA
                     IGRPLIAKKLVETAHKYGATAVAHGCTGKGNDQVRIDIGVRALDPTLEIIAPARAADW
                     PSREELIDYVTERGIPVNLTKKSPYSIDENMWGRSNECGVLEDPWVAPPEDAFQFVKS
                     VSDAPDEAQTVVVSFEKGKPVALDGVPMGMVELIEKIDAIGNAHGFGRVDMIENRLVG
                     IKSREVYEVAGSLALITAHQALEDLVLTRDLLHFKKTIESKLADMIYEGQWFDPLTEA
                     CKAFLFQSQERVTGDVRLKFHKGSCVAEGRQSDFSLYSYKLATYTTEDEFSHTAAAGF
                     IELWGLPIEVWARVGKEHHMMKELEMDEEVETA"
     gene            2325..3710
                     /gene="argH"
                     /locus_tag="VFZ86_14785"
     CDS             2325..3710
                     /gene="argH"
                     /locus_tag="VFZ86_14785"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEX5643583.1"
                     /translation="MTVARSGRFAGERAPAFVALNSSLALDWRLWPEDIEGSVAHARA
                     LLGAGILSAAEFEAIQNGLAAVGREIESGAFVPADADEDVHMAVERRLTELIGDAGAR
                     LHTGRSRNDQVVTDVRLHLRRVAERQDAALAALQGLLVARAADHTETVLPAYTHLQRA
                     QVTSLAQHLLAYFWMLERDRSRFEAARRACLELPLGAGAAVGLDFELDREAEAADLGF
                     ERVAENSLDAVADRDFVVDYLAAAAQLGAHISRLGAELVLWASAEFGFVRLPDAYAGG
                     SSIMPQKKNPDAAELMRAAAPRLTADLGGLLGVLHGLPLAYNTDLREDKRYLFDAVDC
                     LDRLLPVVHGLLDEVTFDEARMAAACDPFLAATDVADYLVERGVPFREAHHLTGGLVR
                     RCLERGEGLAQVPLEDLQALSPAFDEGYYALHEPAAQLARKRSRGGSAPARVREQLER
                     ARAALAARPCC"
     gene            3949..4554
                     /locus_tag="VFZ86_14790"
     CDS             3949..4554
                     /locus_tag="VFZ86_14790"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEX5643584.1"
                     /translation="MTGPQPLPQVFFARSALEVARDLIGCTFLFGGVGGRIVETEAYR
                     QDDPCCHGYRGKTDRNAVLFGPPGHLYVYFTYGMHFCANVASEEEGVGAGVLLRALEP
                     EHGVEDMVARRGVLEPRLLASGPARLAQALGIGRAQNGLPVWEPPLEVLPREADAPGP
                     DVVTTARIGVRGGDQKPWRFVDAGSRFLSRPLSRTPILPSG"
     gene            4591..>4881
                     /locus_tag="VFZ86_14795"
     CDS             4591..>4881
                     /locus_tag="VFZ86_14795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NGG1p interacting factor NIF3"
                     /protein_id="HEX5643585.1"
                     /translation="MKLREIYETAVRMGIEADPRGKEGVDEFLARTKKRYDALPDHLK
                     ELADPEELTNPFNDTRIYVGDPEAEVKTLLGGIDMNTGEVLLADRLREKGVPV"
BASE COUNT          736 a         1835 c         1663 g          647 t
ORIGIN      
        1 ctgggcgcgc tgcaggccgc cggccgcatc ggcggcggca tggtgcccaa ggcggccgcc
       61 gtgcgcaagg cgctcgaggc cggcgtgggg agcgcccaca tcatcgacgg ccgggtcgag
      121 cacgccgtcc tgctcgagat catgaccgac gccggctgcg gcaccaaggt ggtcgcgtga
      181 gccccgcagc cgtcgtcgca gagcagacgg tttgcatatt cccggcgcag gcggtgtatt
      241 ctatgcagcg tatgaaccag cgcgcgccca tgagcaagac cgaacgccag cggctgatcc
      301 agacggtcgt ccagcagcgg gagatcgcca cgcagcggga gctggtgagc gcgctcagcg
      361 ccctgggctg tgaggtcacc caggcgacgg tctcgcgcga catccgcgag ctcgacctgc
      421 agaaggttcg cacgcacctc ggtcgcgcca agtacgtcct cagctcgcgc gagcggccca
      481 aggaccccga gcaggcggcg cgccacgtgc tgcgcgactt cgcccggtcc accgccgtct
      541 cgcagcagct gctggtggtg cgctgcgaga tcgggaccgc gtcgacggtc gcgcggcaga
      601 tcgacaacct caaccacacc gacatcgtcg gcaccctggc cggcgacgac accttcgtgg
      661 tcatcctgga cacgagcgac aaggcgcgcg agatgaagcg ctacgtcgac cgtctccgag
      721 aggcctagcg cgcaggcacg actccgcgcg cgccccatcc tcgtcctcgg gcagccggcg
      781 agcggcttcg ccgcgccggc cccggccccg gcccaccctg actttcaagg agcatcccag
      841 tgaccacacc cagctcaggc aacggcagcg cccccaacgg cacggtcgtc ctcgcgtact
      901 cgggcggcct cgacacctcc gcgctcgtgc cgtggctcaa ggacaagtac ggctatgacg
      961 tggtctgctg cctcgtcgac atcggccgcg tcaaggacgt gcccggcatc atggcgcggg
     1021 ccgaggcggc cggcgccatc gccgccgtcg cgatcgacgc caaggaggag ttcgccgagc
     1081 accactgcct gccggcgctc atggccaacg cgctctacga ggacacgtac ccgctgagca
     1141 cggccatcgg ccggccgctc atcgccaaga agctcgtcga gacggcgcac aagtacggcg
     1201 ccaccgccgt cgcccacggc tgcaccggca agggcaacga ccaggtgcgc atcgacatcg
     1261 gcgtgcgcgc gctcgatccg acgctcgaga tcatcgcacc tgcccgcgcc gcggactggc
     1321 cgagccgcga ggagctcatc gactacgtca ccgagcgcgg catcccggtc aacctgacca
     1381 agaagagccc gtactccatc gacgagaaca tgtggggccg cagcaacgag tgcggcgtgc
     1441 tcgaggaccc ctgggtcgcg ccgcccgagg acgcgttcca gttcgtcaag tccgtgagcg
     1501 acgcgccgga cgaggcgcag accgtcgtcg tcagcttcga gaagggcaag ccggtcgccc
     1561 tcgacggcgt gcccatgggc atggtcgagc tcatcgagaa gatcgacgcc atcggcaacg
     1621 cccacggctt cggccgcgtc gacatgatcg agaaccggct cgtcggcatc aagagccgcg
     1681 aggtctacga ggtcgccggc tcgctggcgc tcatcaccgc gcaccaggcg ctcgaggacc
     1741 tggtgctcac gcgcgacctg ctccacttca agaagaccat cgagagcaag ctggccgaca
     1801 tgatctacga ggggcagtgg ttcgacccgc tcaccgaggc ctgcaaggcg ttcctcttcc
     1861 agagccagga gcgggtcacc ggcgacgtgc gcctcaagtt ccacaagggg agctgcgtgg
     1921 ccgaggggcg ccagagcgac ttctccctgt acagctacaa actcgcgacc tacacgacgg
     1981 aggacgagtt ctcgcacacg gccgccgccg gcttcatcga gctgtggggc ctgccgatcg
     2041 aggtctgggc gcgcgtgggc aaggagcacc acatgatgaa ggagctcgag atggacgagg
     2101 aggtcgagac cgcctgaccg cgggctcgac ccgccgccac ggggaggagc gggcggccgc
     2161 cgcacgggcc gccgcccgcc ggcgctcacg gctgcaccgc cgtccgccgg gggaggtcgt
     2221 cacatggcgg ccgcccgctc cgcacccgtt cgaagacata ggatgcccac aatgacgagc
     2281 actgccacca tcatccgcgc cgcgcggctc gccgggagtc ccgcgtgacc gtcgcccgct
     2341 cgggccgctt cgccggcgag cgcgcccccg ccttcgtggc gctgaacagc tcgctggcgc
     2401 tcgactggcg cctgtggcct gaggacatcg agggctccgt ggcgcacgcc cgggcgctgc
     2461 tgggcgccgg catcctgagc gcggccgagt tcgaggccat ccagaacggc ctcgccgccg
     2521 tcggccgcga gatcgagtcc ggcgccttcg tccccgccga cgccgacgaa gacgtgcaca
     2581 tggccgtcga gcgccggctg acggagctca tcggagacgc cggcgcccgc ctgcacaccg
     2641 gccgcagccg caacgaccag gtcgtcacgg acgtgcgcct gcacctgcgc cgcgtcgccg
     2701 agcgccagga cgcggcgctg gccgccctcc agggcctgct cgtcgcgcgg gcggccgatc
     2761 acaccgagac cgtcctcccg gcgtacacgc acctgcagcg cgcgcaggtc acgtcgctgg
     2821 cgcagcacct gctcgcgtac ttctggatgc tcgagcgcga ccgctcgcgg ttcgaggccg
     2881 ctcgccgggc gtgtctcgag ctgccgctcg gggccggcgc cgccgtcggc ctcgacttcg
     2941 agctggaccg cgaggccgaa gcggccgatc tgggcttcga gcgcgtcgcc gagaactcgc
     3001 tcgacgccgt ggccgatcgc gacttcgtcg tcgactacct cgccgccgcc gcccagctgg
     3061 gcgcccacat ctcgcggctc ggcgcggagc tcgtcctctg ggcgagcgcc gagttcggct
     3121 tcgtgcgcct tccggacgcc tacgccggcg gctcgagcat catgccgcag aagaagaacc
     3181 cggacgccgc cgagctgatg cgggccgccg ctccgcggct gaccgccgac ctcggcggcc
     3241 tcctcggcgt gctccacggc ctgccgctcg cctacaacac cgacctgcgc gaggacaagc
     3301 gctacctctt cgacgccgtc gattgcctcg accggctgct gcccgtcgtg cacgggctgc
     3361 tcgacgaagt gaccttcgac gaggcgcgca tggcggcggc ctgcgatccc ttcctggccg
     3421 ccaccgacgt cgccgactac ctcgtggaga ggggcgtgcc gttccgggag gcgcaccacc
     3481 tcaccggcgg cctggtgcgc cgctgcctcg agaggggaga gggcctcgcc caggtcccgc
     3541 tcgaggacct ccaagccctc tcgccggcct tcgacgaggg ctactacgcg ctccacgagc
     3601 ccgccgcgca gctggcgcgc aagcgctccc gcggcgggtc cgcgcccgcg cgcgtgcgcg
     3661 agcagctcga gcgggcgcgc gccgcactcg cggcgcgacc ctgctgctga cggccgccgc
     3721 tgatcgtgcg ccgtgccgcc cggcgagccc gggcatcagg tagcctccag gcacaaggta
     3781 gtcctctcgg gactggcacg ttccgaggta cgctcaggtg tgacgtcgta cgcttggtag
     3841 tacatgcgag ggtgcgagtc cggagagcac atggtcctcc tgggaagcct tcgggcggcc
     3901 gcgtcggctc gtgccgaacg ccgcccgccg gtccgctaca ctctggtcgt gaccggtccc
     3961 cagccactgc cgcaggtgtt cttcgcgcgc tccgcgctcg aggtggcgcg cgacctcatc
     4021 ggctgcacct tcctcttcgg gggcgttggt gggcgcatcg tcgagacgga ggcctaccgg
     4081 caggacgacc cctgctgtca cggctaccgc ggcaagacgg accgcaacgc cgtgctcttc
     4141 ggcccgcccg gccacctcta cgtctacttc acgtacggca tgcacttctg cgccaacgtg
     4201 gcgtccgagg aggagggcgt gggagcgggc gtgctgctcc gggccctcga gcctgagcac
     4261 ggcgtcgagg acatggtcgc ccgccgcgga gtgctggagc cgcgcctgct cgcctccggc
     4321 ccggcgcggc tcgcccaggc cctcggcatc ggccgcgccc agaacggcct gccggtctgg
     4381 gagccgccgc tggaggtcct gccgcgggag gcggacgcgc ccggtcccga cgtggtcacc
     4441 acggcgcgca tcggcgtccg cggcggcgac cagaagccgt ggcgcttcgt cgacgccggc
     4501 agccgcttcc tctcgcgccc gctctcacgc acgcccatcc tgccctctgg gtaagatagg
     4561 agggattcca gttcaccaga aaggaccacc gtgaagctcc gcgagattta cgagaccgcc
     4621 gtgcgcatgg gcatcgaggc cgacccgcgc ggcaaggagg gcgtcgacga gttcctggcg
     4681 cgcaccaaga agcggtacga cgcgcttccc gaccacctca aggagctcgc cgacccggaa
     4741 gagctcacca accccttcaa cgacacgcgc atctacgtcg gcgatcccga ggccgaggtc
     4801 aagaccctgc tcggcggcat cgacatgaac accggcgagg tgctcctcgc agaccgcctg
     4861 cgcgagaagg gcgtccccgt c
//
LOCUS       DASUMX010000316        14995 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_124372, whole genome shotgun sequence.
ACCESSION   DASUMX010000316 DASUMX010000000
VERSION     DASUMX010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 14995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14995
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124372"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..178)
                     /locus_tag="VFZ86_14800"
     CDS             complement(<1..178)
                     /locus_tag="VFZ86_14800"
                     /inference="COORDINATES: protein motif:HMM:NF018893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 family protein"
                     /protein_id="HEX5643586.1"
                     /translation="MAKYVDGFVLVIPKDKGEEYKKMAESGRDSWMKHGALAYYECRG
                     DDLTPQEMGSEKTRA"
     gene            536..982
                     /locus_tag="VFZ86_14805"
     CDS             536..982
                     /locus_tag="VFZ86_14805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003978625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5643587.1"
                     /translation="MDGRRIRRFDHLGITVADLDAVTAFFVGLGLEVDGRTFVEGEFI
                     DTVTGIPNARSEIVMLRPPGGGTAIELSSFVRPPHEPGSPEAMANELGLRSVAFEVDD
                     LQATVDQLAADGYGLIGGIGQYERIWSMAYVRGPEGIIVALAQRIE"
     gene            complement(1355..2071)
                     /locus_tag="VFZ86_14810"
     CDS             complement(1355..2071)
                     /locus_tag="VFZ86_14810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643588.1"
                     /translation="MRVRIVITVVVAALVLLTLPGAALAWSNGPNGANGFGTHDWVLK
                     EANRLAATQNAGWVRLNVALPKTDDPDTVFHDFYYHVYDVWGSRYGDAPRKVALFYGK
                     ALAARKAGDWRAASKWVGIMAHYYADICNPLHTDQSRAEERMHSAYESAAQRSTDAPG
                     ENRSWVAFNGYRARSDVAGFTKDTAATSHKYYAALVRGYNSGGMNATVLSITRKRVNG
                     AVNGLADLIIAIKRGVKGTR"
     gene            complement(2228..2404)
                     /locus_tag="VFZ86_14815"
     CDS             complement(2228..2404)
                     /locus_tag="VFZ86_14815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643589.1"
                     /translation="MKKLIFLMLLILVGVGAYFAWKMLKGDGGCCGWGGDNTDPWSSY
                     TPPAEDAPAETPAL"
     gene            2549..2977
                     /locus_tag="VFZ86_14820"
     CDS             2549..2977
                     /locus_tag="VFZ86_14820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643590.1"
                     /translation="MWVTTHVLAGLGIAAALGGPWWLILVLVVLAHVLMDLVPHWDYT
                     VSRHPILYGWCDFLASFAAFWIAWLLLGYPWWIAFMGLVSGAPDWDVLIWVARGEKGR
                     KVFPSHWDRFPHGRSGKAWGISVQVAIMAASVAVVLALAP"
     gene            3066..3524
                     /locus_tag="VFZ86_14825"
     CDS             3066..3524
                     /locus_tag="VFZ86_14825"
                     /inference="COORDINATES: protein motif:HMM:NF018184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HEX5643591.1"
                     /translation="MDYEIAEKDIIPQLALTYRQPVTMETIADRIGGAFRALVEHAAK
                     TGASWAGPPFILYPEDCSDEFVITVCMPVVPGATGGEQVALEEVPGGTVASTVHVGPY
                     NGIGKAYVALQKWMTDNGRRPAGMVREIYLNDPDAVPVEELLTEIDWPVA"
     gene            complement(3567..4934)
                     /locus_tag="VFZ86_14830"
     CDS             complement(3567..4934)
                     /locus_tag="VFZ86_14830"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX5643592.1"
                     /translation="MEDIAGIDPGVVAEVERETSELLSALIRIDTSNPPGDETRVAEF
                     MAAWFRERGLEGEIAGEPADRASFVLRVDSGRPGPSLLLLAHEDVVPANAPDWRVPPF
                     SGLIADGYVWGRGAVDIKNLVAAHAVAAARIAAAGGPAAGALVYACTADEEEGSVGGA
                     RWLVHNRPDLVKTDYVINEGGGHFLERAGRRVYLLETGEKGTAQFRVIVKGEAGHASV
                     PLRHGNAVVAAARVVDALVSHRMPVVIDGSSEELVRLLVDSPALRKRLRDPETARAAL
                     IDLARRDVQLADMIEPLYGFAFSPTILHSNSVAVNVYPTRVELTVDCRMLAGHQEHEV
                     LTEVRTALDGVEADWDLEWAGPVVRGNSSPYPSPLAEAIRTTLERHVPGAELANSHSV
                     GFTDSNWFRAAYPDTVAYNFAPHLVERYDEVTPRYHNVDERILVRDLAFQALFAERVA
                     LELLR"
     gene            5053..5146
                     /locus_tag="VFZ86_14835"
     tRNA            5053..5146
                     /locus_tag="VFZ86_14835"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5087..5089,aa:Ser,seq:gct)
     gene            5171..5620
                     /locus_tag="VFZ86_14840"
     CDS             5171..5620
                     /locus_tag="VFZ86_14840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018819362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5643593.1"
                     /translation="MTAIQLTEVTADNWRACADLEVAPDQQAFVAPVTRYLCLCHYGG
                     VWRPLAVTAGDEVVGFVMWAVDPDDGSGWIGGLVIGREYQRRGYGRAAVLALVERLRR
                     DEGCPSAALSHAADNAAARALYASLGFAETGEREDDELVARLRFREP"
     gene            5665..5742
                     /locus_tag="VFZ86_14845"
     tRNA            5665..5742
                     /locus_tag="VFZ86_14845"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5700..5702,aa:Arg,seq:acg)
     gene            5919..6539
                     /locus_tag="VFZ86_14850"
     CDS             5919..6539
                     /locus_tag="VFZ86_14850"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX5643594.1"
                     /translation="MRKLIVNEFVSMDGVAQAPGGQEEDPSGGFAHGGWHMRYTQDER
                     ARAWVMEYIADMGALVLGRRTYEIFAGYWPNASEEEAFIAEPLNRVPKHVASTTLTEP
                     LAWSNSSLLRPDVATALRALKQKDGRDLHVIGSAQLVRGLLADDLVDELRLMIDPLML
                     GGGKRIFPEDGELRRFRLAEHEFTNDGAVLATYSRQREEEAARDAA"
     gene            complement(6690..7136)
                     /locus_tag="VFZ86_14855"
     CDS             complement(6690..7136)
                     /locus_tag="VFZ86_14855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643595.1"
                     /translation="MDSTGIAEGKALFTDHTYDNLGIPANPTVWALTGSDAVDLGLGG
                     FLKNAGYGEAVWMAEAGKFKVPTLRNVARNRPYGHNGSFATLYDIVHFYNTRDVPGAG
                     WAAPEVPETVNTDELGDLGLTKREELALVAFMKTLSDRAFMPQPAH"
     gene            complement(7415..7591)
                     /locus_tag="VFZ86_14860"
     CDS             complement(7415..7591)
                     /locus_tag="VFZ86_14860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643596.1"
                     /translation="MRKVGVVMVALGLSGAAGGYYVAYTADPWADTAGLGIGGALAAV
                     CLAIVGMLLIQFKS"
     gene            7740..9086
                     /locus_tag="VFZ86_14865"
     CDS             7740..9086
                     /locus_tag="VFZ86_14865"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5643597.1"
                     /translation="MKRRRTRGRTSTFARAAVTAALVVPLLAVTAACGDPAPAARATP
                     LPVRIGAIYNLTGDQSALDSPSLDGARLAVERINARGGLLGRRVELLERDGQTSAANV
                     RRAAASLVASGVTAIVGLSDTDQVLAAAPIAARAGVPFVTSGATSPRLTEQVPDWLFL
                     ACFGDNTQAAASAQYASDRLGARTAAVLYDKDLDYTRLLAKYFTRSLRAQGGRVLVSA
                     DFENASHAIQRLDDGGSSSEGAAGEEGEEASGGSDASSGGESAGGEQSSESSGKATDT
                     ANQAATADVLFVAAGPEDAPRIVRDLRAAGYRQPIMGGDSFDSAALISAAEKSGGKVY
                     YTTHSAVGMNTASNAVRRFDASFRAAYGRLPQSAFAGLGYDAVGLVASAVRRADSAEP
                     SKVRDAIQATRHYPGVTGTLSFDGEDRVPRKKVTVVCVGRRPVVVAQFTPGFVADP"
     gene            complement(9120..9797)
                     /locus_tag="VFZ86_14870"
     CDS             complement(9120..9797)
                     /locus_tag="VFZ86_14870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011702711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HEX5643598.1"
                     /translation="MDWTLYIICFGIPLILGLAVQGWLRSTFAKNSRVPVASGMTGAD
                     VARRILDNNGLQSVPVQPSKAGPLSDHYDPRTRGVFLSEAVYAGRSVAATAVAAHEVG
                     HAVQHATAYAPMRLRSAIWPVAAFGSQAWFFILIAGVIFNAFGLVQLALIVFALVIAF
                     QIVTLPVEFNASRRALAQIRDLRLVNDTEAVGARQTLTAAAMTYVAGALASIAQLLYF
                     LAIFGRD"
     gene            complement(9875..10459)
                     /locus_tag="VFZ86_14875"
     CDS             complement(9875..10459)
                     /locus_tag="VFZ86_14875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018347411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX5643599.1"
                     /translation="MATVAETTTRRYEGVEFPVAGTWALDPTHTRVDFEARHLMVAKV
                     KGHFGGAEGALVIGADPTQSTAQVTIATATIDTGVEQRDQHLKSPDFLEVEAHPEIVF
                     TSGSFEHVQDGDWRLHGDLTLHGVTRPVTLKTEYNGVAIDPWGGTRAFFSATTKFDRE
                     EWGLSWNVALETGGWLVGKEIKVAIEVEAVLQQD"
     gene            10588..11052
                     /gene="tadA"
                     /locus_tag="VFZ86_14880"
     CDS             10588..11052
                     /gene="tadA"
                     /locus_tag="VFZ86_14880"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HEX5643600.1"
                     /translation="MGDVHFSRDDYYMRLALREAERAPRHGDVPIGAVVVLDGEVIAA
                     AGNERELRRSPVAHAEMIAIEQAAAHMGSWRLLNTVLYVTLEPCPMCAGAVVQARIPR
                     LVYGATDEKAGAAGTLYNVCQDDRLNHCLDITSGVLADESVALLRQFFQARR"
     gene            11056..12000
                     /locus_tag="VFZ86_14885"
     CDS             11056..12000
                     /locus_tag="VFZ86_14885"
                     /inference="COORDINATES: protein motif:HMM:NF016013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF389 domain-containing protein"
                     /protein_id="HEX5643601.1"
                     /translation="MFHLRAYVPPEQVDDVSRALRARDGARHIVLSGVTADSGAALIT
                     AELDDEVADASVADLTALGVRAADLSISRVQVVRPMTDEGYALETEAQHDTLVWSEVT
                     DEAVDNVGLRPSYLAYMAVAGVIAAFGVLEHSAILIVGAMAVSPDLLPLTAAAVALVG
                     RRPRLLGRALGTLLPGLLTAVVAAWLVTLALRATGVVAAGADIQSGVAYSLAHVSVAT
                     AGVALAAGVAGMLSFETRASFAVGVAISVTTIPAVAYIGVAAGLGEWSGALGAVTVLG
                     VNLAMLLVAGTATLGIQTAISRRDGGGRGTRPHDRQTY"
     gene            12018..13358
                     /locus_tag="VFZ86_14890"
     CDS             12018..13358
                     /locus_tag="VFZ86_14890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016694408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEX5643602.1"
                     /translation="MPTDLRQTIEDLMPRLTEELKQLVRLPSVSFPGFPTAPVEQAGA
                     AVARLFTEAGLPGVRMLDVPRAPQAVYGERPAQPGAPTVLLYAHYDVQPAGPEDAWTS
                     LPFEPVERGGRLYGRGAADDKSGIVTHAGALLALGEDCPVGVKVLIEGQEEADGGGIE
                     EFVRENAALLQADVVVVSDLGNYALGVPTLTTSLRGMAACDVEVETLEGAVHSGMFGG
                     PAPDALVALSRMIATLHDDKGDVAVTGLSTIAYEGAPYDEAAYRADAGVLDGVGLIGS
                     GTIAERLYGKPSINVIGIDAPRVDGAANALVPRARARVSVRLAPGQKPAAAQEAVRAH
                     LEAAAPWNVRVRVTAGGVGEGFVARTDGPAYAAATGAMAAAYGKDVVRYGEGGSIPLV
                     AAFQAALPHAEMILWGTEEPRCKIHAPDESVDLGELERCTVAEALFISALAART"
     gene            13494..13587
                     /locus_tag="VFZ86_14895"
     tRNA            13494..13587
                     /locus_tag="VFZ86_14895"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13529..13531,aa:Ser,seq:gga)
     gene            13719..14921
                     /locus_tag="VFZ86_14900"
     CDS             13719..14921
                     /locus_tag="VFZ86_14900"
                     /inference="COORDINATES: protein motif:HMM:NF024211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX5643603.1"
                     /translation="MTDGRADQVSRKRSRWARWIVLGVVLVGMTAIVYSHQKAQGGGR
                     WAGVDFLCPFGGLETLYSLLTGDGFLRRTAASSVILLVGMLAMAVAYRRSFCGNLCPL
                     GALQGLFGAAGRRLLRRRLTVPRAVDRVARYLKYAVLVFFALWTWQAAELVLRPYDPW
                     VAWAHLTSPELLTSYGIGLAVLIVSLAGSLVYERFFCKYLCPAGALLGLLSRVSFLNI
                     RRDADACIDCGRCDKACMMNVEVATAGAVTSSECISCNECVNACPVPGALKVTAPGGA
                     RATALVATGAVVALMVAIVGVTTLTGAFDWRMEGRAEARGAGHSGGGEGLGQGEGHGG
                     GGSDSPIKGWMSLADIAAMTGIPLEEFAAEWGVPAEALGLPMKDVKDQYGFSPDDVRA
                     WVEDRLAE"
BASE COUNT         2152 a         5354 c         5259 g         2230 t
ORIGIN      
        1 atgcgcgggt cttctcgctg cccatttcct ggggggtcag gtcgtcgcct cggcattcgt
       61 aatacgccag cgccccgtgt ttcatccagg aatcacgtcc gctttctgcc atcttcttgt
      121 actcttcacc cttgtccttg gggatcacaa gcacgaatcc gtccacatac ttcgccatgt
      181 cgtagtaccc cttctcgatt gagcaatcgt cactacgata cccagcgtgg gctgacgtct
      241 actgatagga actgtcatcc cagcgtgggc tgacgttcgc ctgcctgctg atcgaggcgg
      301 gcgtcgacct ctacaccgtc tcccgccggc tcgggcacgc gacgacggcg gtcaccgagc
      361 tgcactacgt cgacccttca gagaaggccg acgaggcagc cgccgacgcg ctcggcgacc
      421 tgcgcctgcc ggcgccggag gccagggcgc ccgccggccg cttcccccag gcgaaggggt
      481 aggcagcgta cgctcttttg cgctgcggtc ctccgccggc gggcagagtg cgagcatgga
      541 cgggagacgc atccggcgct tcgaccacct cggcatcacg gtcgccgacc tcgacgctgt
      601 gacggcgttc ttcgtcgggc tgggtctcga ggtcgacgga aggacgttcg tagaaggcga
      661 gttcatcgac accgtcaccg gcataccgaa cgcccgcagc gagatcgtca tgctcaggcc
      721 gccgggcgga gggaccgcga tcgaactctc gagcttcgtc cggccccctc acgagccagg
      781 gtcgcccgag gcgatggcca atgaactggg gctgcgtagc gtcgccttcg aggtggatga
      841 cctccaggcg acagtcgacc agctggctgc cgacggctac ggcctgatcg gcggcatcgg
      901 tcagtacgag cggatctgga gcatggcgta cgtgcgcgga ccggagggga tcatcgtggc
      961 cctggctcag cggatcgagt gatccgcgcg cgcccgaggt cgccgggcaa gagcgccgtc
     1021 cctgcctctc cactcggctc tgcgacgccg aacctccttc tggggagggc ccgacaaggg
     1081 cgaccccgag gccctcttcg gcgcgcgcca ttctgcgcca tgtgccaaaa gacgccacgt
     1141 cgagaggccc cggtcggcga ccaagaacga cggtgagcga ccgtgaccgc actccgctcc
     1201 cgaatagctc cgtttgcagg tactttgtac agggggaagg cgacttgaaa accgccaacg
     1261 gggcgactcg ttcgtgggtt cgaatcccac cccctccgcc aagaccgaag gatcgacgcg
     1321 ctcatcgtgg acgaagcgaa ggctgagtct cgaactagcg cgtgcccttg acgcccctct
     1381 tgatcgcgat gatgaggtcc gcaagaccgt tgacggcccc gttcacgcgc tttcgcgtga
     1441 tggagaggac ggtggcgttc atgccgccgc tgttgtagcc cctgaccagg gccgcgtagt
     1501 acttgtgact cgtcgccgcc gtgtccttgg tgaagcccgc gacatcgctc ctcgcccggt
     1561 agccgttgaa ggcgacccag gaccggttct cgccgggggc gtcggtggag cgctgagcgg
     1621 cggattcgta ggcggagtgc atgcgctcct cggccctgct ctggtcggtg tgcagcggat
     1681 tgcagatgtc ggcgtagtag tgggccatga tgccgaccca cttgctggcg gcgcgccagt
     1741 cgccggcctt gcgcgccgcc agggccttgc cgtagaagag ggcgaccttc cggggcgcgt
     1801 ccccgtacct gctgccccac acgtcgtaga cgtggtagta gaagtcgtgg aagacggtgt
     1861 cggggtcgtc ggtcttcggc agggcgacgt tgaggcgcac ccagccggcg ttctgcgtgg
     1921 ccgccagccg gttcgcctcc ttcagcaccc agtcgtgcgt gccgaagccg ttggcgccgt
     1981 tggggccgtt gctccaggcg agggcggcgc cgggcagggt gagcaggacg agcgccgcga
     2041 cgacgacggt gatgacgatg cggacgcgca cgatccctcc ctgcggttgc gaaccggttg
     2101 acgatcaccc tacacgacac cgcggacgga ggccgagccg tcgggttccg ccgggcgtac
     2161 gcgtgacggc gccgggaggc gacgaggcaa aggcctcgcc tcccggcgcc gtcacgacag
     2221 agtcagatca gagcgcgggc gtctccgcgg gcgcgtcctc cgccggcggg gtgtagctgc
     2281 tccacggatc cgtgttgtcg ccgccccagc cgcagcagcc gccgtcaccc ttcagcatct
     2341 tccaggcgaa gtacgcgccc acgccgacga ggatgagcag catcaggaaa atcagcttct
     2401 tcaagggtcc ctcctccggt tcgctgtgcc catagtaccc tatgccgccc gccgctaccc
     2461 cgcatccgta cgatgaccgg gcgtcgacgc gtcggcgccc gcactcgccc tctggcgcgc
     2521 cgcgggcgcg atgccgtagt ctgccgccat gtgggtcacg acacacgtcc tcgccggcct
     2581 cggcatcgcc gccgccctcg gaggtccctg gtggctcatc ctcgtcctgg tggtgctcgc
     2641 gcacgtgctc atggacctgg tcccgcactg ggactacacc gtctcgcgcc accccatcct
     2701 ctacggctgg tgcgacttcc tcgcctcgtt cgccgccttc tggatcgcgt ggctgctgct
     2761 cggctacccc tggtggatcg cattcatggg gctcgtcagc ggcgcgccgg actgggacgt
     2821 gctcatctgg gtcgcgcgcg gtgagaaggg ccgcaaggtg ttccccagcc actgggaccg
     2881 cttcccgcac ggccggtccg gcaaggcctg gggcatctcg gtgcaggtcg cgatcatggc
     2941 cgcgagcgtc gccgtcgtgc tcgccctggc gccctgacgg ccgacagcgc cgccgttccc
     3001 cgcaccggag ccgcacggta tcctcaaggc cggggaccgg ccgcgccacg aagggagacg
     3061 agagcatgga ctacgagatc gccgagaagg acatcatccc gcaactggca ctcacgtacc
     3121 gccagccggt gacgatggag acgatcgcgg accgcatagg cggcgcgttc cgcgcgctcg
     3181 tggagcacgc ggccaagacc ggcgccagtt gggccggtcc gccgttcatc ctgtaccccg
     3241 aggactgcag cgacgagttc gtgatcaccg tctgcatgcc ggtcgtgccc ggcgcgaccg
     3301 gcggcgagca ggtcgcgctc gaggaggtgc cgggcgggac ggtcgcgtcc acggtgcacg
     3361 tcggtccgta caacgggatc ggcaaggcct acgtcgcgct gcagaagtgg atgacggaca
     3421 acggccggcg gccggcgggg atggtgcgag agatctacct caacgatccg gacgcggtgc
     3481 ccgtggagga gctcctgacc gagatcgact ggccggtggc gtaggggcgg gccggggagc
     3541 gatccccagc ggagcagctc cagccgtcag cgaagcaact ccagcgcgac ccgctccgcg
     3601 aacagggcct ggaaggccag gtcgcggacg aggatgcgct cgtcgacgtt gtgatagcgc
     3661 ggcgtgacct cgtcgtagcg ctccacgagg tgaggggcga agttgtaggc gacggtgtcg
     3721 ggatacgcgg cgcggaacca gttggagtcg gtgaagccga cggagtggct gttggcgagc
     3781 tcggcgccgg gcacgtgccg ctcgagcgtc gtgcggatcg cctcggcgag gggcgaaggg
     3841 tacggcgacg aattgccccg caccaccggg ccggcccact cgaggtccca gtctgcctcc
     3901 accccgtcca gcgcggtgcg gacctcggtg agcacctcgt gctcctggtg cccggcgagc
     3961 atccggcagt ccacggtcag ctcgacgcgg gtggggtaga cgttcacggc gacgctgttg
     4021 ctgtggagga tcgtcggcga gaaggcgaag ccgtacagcg gctcgatcat gtcggcgagc
     4081 tggacgtcgc ggcgcgcgag gtcgatcagg gccgcgcgcg ccgtctccgg gtcgcgcagg
     4141 cgcttgcgca gggcgggcga gtcgacgagc agccgcacca gctcctcgga cgacccgtcg
     4201 atcaccacgg gcatccggtg cgagaccagc gcgtcgacga cgcgcgccgc ggccacgacg
     4261 gcgttgccgt ggcgcagcgg gaccgacgcg tggcccgcct cgcccttgac gatcacgcgg
     4321 aactgcgccg tgcccttctc accggtctcg aggagataga cgcggcgccc ggcccgctcg
     4381 aggaagtgcc ccccgccctc gttgatcacg tagtcggtct tcacgaggtc ggggcgattg
     4441 tggacgagcc agcgggcgcc gccgacggac ccctcctcct cgtcggcggt gcaggcgtag
     4501 acgagcgcgc cggcggccgg gccgccggcg gcggcgatcc gcgcggccgc cacggcgtgc
     4561 gcggcgacca ggttcttgat gtcgaccgcg ccgcggcccc agacgtagcc gtccgcgatc
     4621 aggcccgaga acggcggcac gcgccagtcg ggcgcgttgg ccggcaccac gtcctcgtgc
     4681 gcgagcagca gcagcgaggg gccgggcctg ccggagtcca cgcgcaggac gaagctcgcg
     4741 cggtcggcgg gctcgccggc gatctcgccc tcgagccctc gctcacggaa ccaggcggcc
     4801 atgaactccg ccaccctggt ctcgtccccg ggcgggttcg acgtgtcgat ccggatgagc
     4861 gcgctcagca gctcgctggt ctcgcgctcc acctcggcga cgacgccggg atcgatgccg
     4921 gcgatgtcct ccactcggac cccccagtac tccctgtgac ggtgcggtaa gcgtcatcat
     4981 atccgccgcg tgcccggacc accgcccagc tttcgagccc gtggcgggtg cggtatagtg
     5041 tgtctcccgc gcggagaagt ggccgagtgg ctgaaggcaa ccgcctgcta agcggttaag
     5101 ggggctttaa cctccttccc gggttcgaat cccggcttct ccgccagtct ccccgacctc
     5161 cgggagcgac gtgaccgcga tccagctcac cgaggtgacc gccgacaact ggcgcgcctg
     5221 cgccgacctc gaggtcgctc ccgaccagca ggccttcgtc gctcccgtca cccgctacct
     5281 gtgcctctgc cactacggcg gcgtgtggcg cccgctcgcg gtgacggccg gtgacgaggt
     5341 cgtcggcttc gtgatgtggg ccgtcgaccc cgatgacggc agcggctgga tcggagggct
     5401 cgtgatcggc cgcgagtacc agcggcgagg gtacgggaga gccgccgtgc tcgccctcgt
     5461 cgagcggctg cgccgcgacg agggctgccc ctcggcggcc ctcagccacg ccgcggacaa
     5521 cgcggccgcc cgcgcgctct acgcatcgct cggcttcgcc gagaccggcg agcgtgagga
     5581 cgacgagctc gtcgcgcggc tccgcttccg cgagccctga cgttttcccc tccccgcgct
     5641 ttcgggtata ctctcggtcc gcgtgcgcac gtagctcagt tggatcagag cgtcggtcta
     5701 cggaaccgaa ggccgcaggt tcgagtcctg ccgtgcgcac cagctcttcc cctcgcagac
     5761 ccctctcccc tcgtcacccc ctctttccgc cggcggctcc gcatggccgg atcggtcggt
     5821 gaggccgttt ctcaccgtcc gggccgggaa ccgtcctctc cagtgggatc gcacccggcg
     5881 gtcctctcag gcaggcgatc ccgtcgggga ggaacacgat gcgcaagctg atcgtcaacg
     5941 aattcgtgag catggacggc gtggcccagg cgcccggcgg acaggaggag gacccctcgg
     6001 gcggcttcgc gcacggcggc tggcacatgc gctacacgca ggacgagcgg gcgcgggcct
     6061 gggtgatgga gtacatcgcc gacatgggcg ccctcgtcct tgggcggcgc acctacgaga
     6121 tcttcgccgg ctactggccg aacgcgtccg aggaggaggc cttcatcgcc gagccgctca
     6181 acagggtgcc gaagcacgtg gcctcgacca cgctgacgga gcctctggcg tggagcaact
     6241 cgagtctgct ccgccccgat gtcgccaccg ccctgcgggc gctcaagcag aaggacggcc
     6301 gggacctgca cgtgatcggc agcgcccagt tggtgcgcgg cctgctcgcc gacgacctgg
     6361 tcgacgagct gcgcctgatg atcgacccgc tcatgctggg tggcggcaag cgcatcttcc
     6421 ccgaggacgg cgaactgcgc cggttccgac tggcggagca cgagttcacg aacgacggcg
     6481 ccgtgctggc gacctactcg cgacagcgag aagaagaggc ggcgcgagat gctgcctgaa
     6541 gaacggcgag tccgcctccg cagggacggc ggccgccgcc aagtcctatg aacgacgagg
     6601 gggggcggcg cccatccggc gccgcccccc ccttgtgctt tgtccgacga aacgacggtc
     6661 actgcccgtc aggtcgccct ccccgcagct cagtgagcag gctgcggcat gaacgcgcgg
     6721 tcgctcaggg tcttcatgaa agcgacgagc gccagctcct cgcgcttcgt caggccgaga
     6781 tcgccgagct cgtccgtgtt gaccgtctcg ggcacctcgg gcgccgccca gcctgcaccg
     6841 gggacgtcgc gcgtgttgta gaagtgcacg atgtcgtaga gcgtggcgaa ggagccgttg
     6901 tggccgtagg gccggttcct ggcgacgttg cgcagcgtgg gcaccttgaa cttgccggcc
     6961 tcggccatcc agacggcctc gccataccct gcattcttga ggaacccgcc tagaccaagg
     7021 tcgacagcgt cgctgccggt cagcgcccag acggtggggt tcgcagggat gcccaggttg
     7081 tcgtaggtgt ggtcggtgaa cagcgccttg ccttcggcga tgcccgtcga gtccatggtc
     7141 ggctcgctca ggtgccacaa cgcttgtggt aaccgccgaa ggcgcacagt atgaaggtgc
     7201 ccgtcgcacg cggcgtagag cgaggcgtgg cacgacaggg ttcacgacct tggcgtgaca
     7261 cgctcgagag cgtcaaggca ggcacgtctt ggacggctgc cttgtctggg ttctcccgag
     7321 ttgcgacggg ctttctgccg ccgtgctact tgtccaggcg tggtacccca tcgcgagacc
     7381 aagaaggcgc gactaccacc tggcgacgta gacgtcagga cttgaactgg atgagcaaca
     7441 tcccgacgat ggcaaggcag actgctgcga gcgccccacc gatgccgagg cccgcggtgt
     7501 ccgcccaagg atccgcggtg tacgcgacgt agtaaccgcc cgcagcgccg gagagcccga
     7561 gggcaaccat gaccacgccg accttgcgca caattccctc cgaacacccg cctgtcacca
     7621 ccactgacgc tacctcctgg ccatagcggt gctgtgaagt ggctgtgaag gattgctaag
     7681 ggtcgcacgg gccccgggat gaagccctcg ctccgctgct ctaccatggg tcgcgaccca
     7741 tgaagcgccg gaggacacgc ggcaggacct cgacgtttgc gcgcgcggcc gtcacggcgg
     7801 cgctcgtcgt gccgctgctc gccgtcacgg ccgcgtgcgg ggaccccgcg cccgcggcca
     7861 gggcgacgcc gcttcccgtg cgcatcggcg ccatctacaa cctcaccggc gaccagtccg
     7921 cgctcgactc gccgtcgctg gacggcgccc gtctcgccgt ggagcgcatc aacgcgcgcg
     7981 gcgggttgct cggccggcgc gtcgagctcc tggagcgcga cggccagacc agcgcggcga
     8041 acgtgcggcg cgccgccgcc agcctcgtcg cctccggcgt caccgccatc gtcggcctct
     8101 ccgacaccga ccaggtgctc gccgccgcgc ccatcgccgc gcgtgccggc gtgcccttcg
     8161 tcaccagcgg cgcgacctcg ccgcgcctca ccgagcaggt cccggactgg ctgttcctcg
     8221 cctgcttcgg cgacaacacc caggcggccg ccagcgccca gtacgccagc gaccggctcg
     8281 gagcgcgcac cgccgccgtg ctctacgaca aggacctgga ctacacccgt ctgctggcca
     8341 agtacttcac gaggtccctc cgcgcgcagg gtggccgcgt cctggtctcg gccgacttcg
     8401 agaacgcctc ccacgccata cagcgcctgg acgatggtgg atcgtcgagt gagggcgctg
     8461 cgggtgagga aggcgaggag gcaagtggcg gctccgacgc gtcgagcggc ggcgagtcgg
     8521 cgggcggaga gcagagcagc gagtcgagcg ggaaggcgac cgacacggcg aaccaggcgg
     8581 cgaccgccga cgtgctcttc gtcgccgccg gccccgagga tgcgccgcgc atcgtccgcg
     8641 acctgcgcgc ggccgggtac cggcagccca tcatgggcgg cgacagcttc gactccgccg
     8701 ccctcatcag cgccgcggag aagtccggcg gcaaggtcta ctacaccacg cactccgccg
     8761 tcggcatgaa cactgcgagc aacgcggtgc gccgcttcga cgcgtcgttc cgggccgcat
     8821 acggccgcct gccccagagc gcgttcgcgg gcctcggcta cgacgcggtc ggcctcgtcg
     8881 cctcggcggt gaggcgggcg gactcggccg agccgtcgaa ggtccgtgac gccatccagg
     8941 cgacgcgcca ctaccccggg gtcacgggca ccctgagctt cgacggcgag gacagggtgc
     9001 cgcgcaagaa ggtgaccgtg gtgtgcgtgg ggcgacgacc ggtggtcgtc gcccagttca
     9061 cgccggggtt cgtcgccgac ccgtgagcgc cgggccggcg ggacgcccgt cccgccggat
     9121 cagtcccgcc cgaagatcgc caggaagtac agcaactggg cgatcgacgc cagcgcgccg
     9181 gcgacgtagg tcatggcggc ggccgtcagg gtctgccggg cgccgacggc ctccgtgtcg
     9241 ttcaccagcc ggaggtccct gatctgcgcc agcgcccgcc gcgaggcgtt gaactccacc
     9301 ggcagcgtca cgatctggaa ggcgatgacc agggcgaaca cgatcagcgc cagctggacg
     9361 agcccgaacg cgttgaagat gacgccggcg atgaggatga agaaccacgc ctgcgagccg
     9421 aacgccgcga ccggccagat ggcggagcgg agccgcatcg gcgcgtacgc cgtggcgtgc
     9481 tggacggcgt gcccgacctc gtgcgcggcg acggccgtgg ccgcgaccga acgcccggcg
     9541 tacacggcct cggagaggaa gacaccgcgc gtgcgcgggt cgtagtggtc gctcagcggg
     9601 ccggccttcg acggctgcac cgggacgctc tgcagcccgt tgttgtcgag gatccggcgg
     9661 gcgacgtcgg ctccggtcat gccgctggcc accggcacgc ggctgttctt ggcgaaggtg
     9721 ctgcggagcc agccctgcac cgcgagccca aggatgagcg ggatgccgaa gcagatgatg
     9781 tagagagtcc agtccatgga gccggttcac ctcacgggag tagtgccgtt cgcctcgcta
     9841 ctatacccgc cgatgggggt cgaccatgac ccggtcagtc ctgctgaagg acggcctcca
     9901 cctcgatggc gaccttgatc tccttgccga cgagccagcc gcccgtctcg agcgccacgt
     9961 tccagctgag gccccattcc tcccggtcga acttcgtcgt cgcggagaag aacgccctgg
    10021 tgccgcccca cgggtcgatg gccacgccgt tgtactccgt cttgagggtg acgggcctcg
    10081 tcactccatg gagcgtcagg tcgccgtgga gcctccagtc cccgtcctgg acgtgctcga
    10141 acgagccgga ggtgaagacg atctccggat gtgcctcgac ctcgaggaag tccggcgact
    10201 tgaggtgctg gtcgcgctgc tcgacgccgg tgtcgatggt cgccgtcgcg atcgtgacct
    10261 gggccgtcga ctgcgtcggg tccgcgccga tgacgagcgc accctcggcg ccgccgaagt
    10321 ggcccttgac cttggccacc atgagatgcc gggcctcgaa gtccacccgg gtgtgcgtcg
    10381 ggtccagagc ccaggtcccg gccacgggga actcgacgcc ttcgtacctg cgggtggtcg
    10441 tctctgctac cgtcgccatg tcagtgcctc ctgtaggtga gccggaaccc ctcggttccg
    10501 gctaactatt tgcgtatgca aatatgtctc cgtttgtgcc cgcgcctgat ggttctcaca
    10561 cacggccgat tcgggcatga tgccgccatg ggtgacgtgc acttctcacg ggacgactac
    10621 tacatgcggc tggcgctgcg cgaggcggag cgcgcgccgc ggcacggcga cgtgcccatc
    10681 ggcgcggtcg tcgtgctcga cggcgaggtg atcgccgcgg cgggcaacga gcgcgagctg
    10741 cgccgcagcc ccgtggcgca cgcggagatg atcgcgatcg agcaggcggc ggcgcacatg
    10801 ggcagctggc ggctcctcaa caccgtgctc tacgtgaccc tggagccgtg cccgatgtgc
    10861 gccggcgccg tcgtccaggc gcgcatcccc cggctggtgt acggcgccac ggacgagaag
    10921 gccggcgccg ccggcaccct ctacaacgtc tgccaggacg accgcctcaa ccactgtctg
    10981 gacatcacca gcggcgtgct ggccgacgag tccgtggccc tcctgcggca gttcttccag
    11041 gcgcggcgct aggtcgtgtt ccacctccgc gcctacgtcc cgcccgagca ggtcgacgac
    11101 gtctcgcgcg cgctgcgcgc ccgggacggc gcgcgccaca tcgtcctctc cggcgtcacc
    11161 gccgacagcg gcgcggcgct gatcacggcg gagctcgacg acgaggtcgc cgacgcctcc
    11221 gtggccgacc tcaccgcgct cggcgtgcgc gccgccgacc tctccatctc gcgcgtccag
    11281 gtcgtgcggc cgatgacgga cgaaggctac gccctggaga cggaggcgca gcacgacacg
    11341 ctcgtctgga gcgaggtcac cgacgaggcg gtcgacaacg tcggcctccg cccgagctac
    11401 ctcgcctaca tggcggtcgc cggcgtgatc gcggcgttcg gcgtgctcga gcacagcgcc
    11461 atcctgatcg tgggcgccat ggcggtgagc ccggacctgc tgccgctcac ggccgccgcc
    11521 gtcgccctcg tgggccgccg gccgcgcctg ctgggccggg cgctcggcac gctcctgccc
    11581 ggcctgctga ccgccgtcgt cgccgcgtgg ctggtcaccc tggcgctgcg agcgaccggc
    11641 gtggtcgccg caggcgccga catccaatcc ggcgtcgcct acagcctcgc ccacgtgtcc
    11701 gtcgccaccg cgggcgtcgc cctcgccgcc ggcgtcgccg gcatgctcag cttcgagacc
    11761 cgcgccagct tcgccgtcgg cgtcgccatc tccgtcacca cgatcccggc ggtcgcctac
    11821 atcggcgtcg ccgccgggct cggcgagtgg agcggggcac tgggcgccgt cacggtgctc
    11881 ggcgtcaacc tcgccatgct cctggtcgcc gggacggcca cgctcggcat ccagacggcg
    11941 atctcgcgac gcgacggcgg cggacggggc acgcggcccc acgaccggca gacgtactga
    12001 acgacaagga gaacgcgatg cccacggacc tgcggcagac gatcgaagac ctcatgcctc
    12061 gcttgaccga ggagctcaag cagctcgtgc gtctgccgtc ggtctcgttc ccgggcttcc
    12121 ccacggcgcc ggtcgagcag gcgggcgcgg cggtggcgcg gctcttcacc gaggcgggcc
    12181 tgccgggcgt gcgcatgctg gacgtgccgc gcgcgccgca ggcggtatac ggcgagcgcc
    12241 cggcgcagcc gggcgccccg accgtcttgc tctacgcgca ctacgacgtg caacccgccg
    12301 gccccgagga cgcgtggacg agcctgccgt tcgagccggt cgagcgcggc ggccggctct
    12361 acggtcgcgg cgccgccgac gacaaaagcg gcatcgtgac gcacgccggc gcgctcctcg
    12421 ccctggggga ggactgcccg gtgggcgtca aggtcctcat cgaaggccag gaggaggcgg
    12481 acggcggcgg catcgaggag ttcgtcaggg agaacgccgc gctgctccag gccgacgtcg
    12541 tcgtcgtcag cgatctgggc aactacgcgc tcggcgtgcc cacactcacg acgtcgctcc
    12601 gcggcatggc cgcctgcgac gtcgaggtcg agactcttga gggcgccgtg cacagcggca
    12661 tgttcggcgg cccggcgccc gacgcgctcg tggcgctgag ccgcatgatc gccacgctgc
    12721 acgacgacaa gggcgacgtg gccgtcacgg gcctctcgac gatcgcctac gagggcgccc
    12781 cctacgacga ggccgcgtat cgcgcggatg ccggcgtgct ggacggtgtg gggctcatcg
    12841 gcagcgggac catcgccgaa cgcctctacg gcaagccgtc gatcaacgtg atcggcatcg
    12901 acgcgccgcg ggtggacggc gccgccaacg cgctcgtccc cagagcccgg gcgcgcgtca
    12961 gcgtgcgcct cgcgcccggt cagaagcccg cggccgcgca ggaggccgtc agggcgcatc
    13021 tcgaggcggc cgcgccctgg aacgtcaggg tgagggtgac ggccggcggc gtcggcgagg
    13081 gcttcgtcgc caggaccgac ggaccggcct acgcggcggc gaccggagcc atggccgccg
    13141 cctacggcaa ggacgtggtg cgctacggcg aaggcggctc gatccccctg gtcgcggcgt
    13201 tccaggcggc gctgccccac gccgagatga tcctctgggg cacggaagag ccgcggtgca
    13261 agatccacgc gcccgacgag agcgtcgacc tgggtgagct cgagcgctgc accgtcgccg
    13321 aggccctgtt catctcggcg ctggccgccc ggacctgagg aagagatccg gtcgtagcga
    13381 cgcgcccgag cggacccgcc cgcccaggcg gaccggccgc ccggccggta cccccgcccg
    13441 acggccctgt cgtatactgt tcgtccgctc gccgggcgcg cccggcagcg cgcggagagg
    13501 tgtccgagct ggcttaagga gcgcgactgg aaatcgcgtg ggcggggtca acctgcctcg
    13561 agggttcgaa tccctccctc tccgccactt cccttcttca caacctctcc agaaccgctt
    13621 cagaagctgt tcacaactcg cctttaggct gtctgcggcc acagacgcac ggccaagagc
    13681 gacggcggtc agaacacatg gcacgggagg acgagggcat gacggacgga cgagcggacc
    13741 aggtgtcacg gaagagatcg cgttgggcgc gctggatcgt gctgggcgtg gtcctggtcg
    13801 gcatgacggc gatcgtctac tcacatcaga aggcgcaagg cggcggacgc tgggccggcg
    13861 tcgacttcct ctgccccttc ggcggcctcg agaccctgta ctcgctcctc accggggacg
    13921 ggtttctgcg gcgcaccgcc gcgagctccg tgatcctgct cgtcggcatg ctcgcgatgg
    13981 ccgtagccta ccggcgctcg ttctgcggca atctgtgccc gctcggcgcg ctgcaagggc
    14041 tgttcggcgc cgccggccgc aggctcctgc ggcggcgcct caccgtcccg cgcgccgtcg
    14101 accgcgtggc ccgctacctc aagtacgcgg tcctcgtctt cttcgctctc tggacctggc
    14161 aggccgccga gctggtgctg cgcccctacg acccctgggt ggcctgggcg cacctcacct
    14221 cgccggagct gctgaccagc tacggcatcg gcctcgccgt gctgatcgtc tcgctggccg
    14281 gctcgctcgt gtacgaacgc ttcttctgca agtacctctg ccccgccggc gccctgctcg
    14341 ggctcctgtc gagggtgagc ttcctgaaca tccgccggga tgcggacgcc tgcatcgact
    14401 gcggccgctg cgacaaggcc tgcatgatga acgtcgaggt ggcgaccgcc ggcgcggtca
    14461 ccagctccga gtgcatctcc tgcaacgagt gcgtcaacgc ctgccccgtg cccggcgccc
    14521 tgaaggtgac ggcccccggc ggcgcccgcg ccacggcgct cgtcgcgacc ggcgcggtgg
    14581 tggcgctgat ggtggccatc gtcggtgtga ccacgctcac cggcgcgttc gactggcgga
    14641 tggagggccg ggccgaggcc cgcggcgccg gccactccgg tggaggcgag ggcctgggtc
    14701 agggcgaggg tcacggcgga ggcggcagcg actcgccgat caagggctgg atgtcactcg
    14761 ccgacatcgc cgcgatgacg ggcatccccc tggaggagtt cgccgcggag tggggcgtgc
    14821 ccgccgaggc cctgggcctg ccgatgaagg acgtcaagga ccagtacggc ttctcgccgg
    14881 acgacgtccg cgcgtgggtg gaggaccgcc tcgccgagtg accggcgcgt ccgcgcgcac
    14941 cagcccgttt gcccggcgca tccgcggcgc gtgagccggg ccgcgggcgc atgct
//
LOCUS       DASUMX010000317        29903 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_413767, whole genome shotgun sequence.
ACCESSION   DASUMX010000317 DASUMX010000000
VERSION     DASUMX010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 29903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29903
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413767"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="VFZ86_14905"
     CDS             <1..475
                     /locus_tag="VFZ86_14905"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEX5643604.1"
                     /translation="RWPAKHDVIFWYARDPARYVFRYDDIDRVPYLAPGLVGPEKAAR
                     GMTPTDVWWQTIVSPTGAEKTGYPTQKPVKILERIVRVHSDPGDLVVDPFAGSGTAGE
                     AAARLGRGYLLVDENPEAVRVMRERLAWDGPETVSGTGPAGGSASSPAPPAEPGP"
     gene            complement(539..709)
                     /locus_tag="VFZ86_14910"
     CDS             complement(539..709)
                     /locus_tag="VFZ86_14910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010266056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX5643605.1"
                     /translation="MAYKITDECLACGACIDECPNEAISEGDDIYVIDPEKCDDCATC
                     VESCPSDAIVPE"
     gene            complement(820..1833)
                     /locus_tag="VFZ86_14915"
     CDS             complement(820..1833)
                     /locus_tag="VFZ86_14915"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX5643606.1"
                     /translation="MAPERIVVGVDIGGTKVAAALVRGRAPAPGARSRSGVETPEILD
                     RFTTLTDVSSAEACLRGIDACLADLEHGSGRVEGIGVGVASMVDFAGGRVVESVNLPL
                     TDVPLRDHLERRFGVPVVVDNDATAAAIGEHAYGAGAGTSDMLMLTLGTGVGGGIISG
                     GRPLRGASGAAAEIGHMIIDVDGPKCPANCPNHGCLEAYVAGPAMGAAARAAAGRAPD
                     SALGRALTEGRTVDSRLLTQLAREGDDGAIAVLERAGEYLGAGLVTLSNIFDPELIVI
                     GGGAAAAGELLLAPARRVLAARALPPARDRVRVVPAALGPDAGFIGAAALALTELFPG
                     DAG"
     gene            1980..3161
                     /locus_tag="VFZ86_14920"
     CDS             1980..3161
                     /locus_tag="VFZ86_14920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEX5643607.1"
                     /translation="MARQDEDREGAKPYKTYKAGRGRRSQLDDELAGARPARTRPDGD
                     ADRGPAYPRQSDKAYHTYGPAPADGRAAGTNKSRGAGAGPRRRRRFRWWYVPVGVFAL
                     LVIAGVAATVLAWPGYQKFDRAVDKANKRVDGQTRAQLAADDGWLWRNGTTVALFGLD
                     EAGLPAHSDTILLMHFDAKSHTINQLSIPRDTLVNVEGYGPQKITQAMWYGGPSLALK
                     TLKEYTGIPINHIMVVSFQGFPRLVNSVGGIDMYVPQTVQTEAGSNRRLVTFKKGMHH
                     FDGKNAMLYVRIRKAYAEGDFTRAKRQQAFVDALQKKIVKPSNITRLPDIGRHFMSGV
                     ATDLTTNQILELAYLKWRAGGGKKQVLKGELGWSDDGQAVVLPPSDAARQKAVARFLG
                     E"
     gene            complement(3214..4011)
                     /gene="truA"
                     /locus_tag="VFZ86_14925"
     CDS             complement(3214..4011)
                     /gene="truA"
                     /locus_tag="VFZ86_14925"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEX5643608.1"
                     /translation="MDGSQETSGGTTWRLDLAYEGTRYRGWARQPGQTTVESLLGDAL
                     ETVLRERVRLSVAGRTDAGVHAWAQVASFTCARSDLSPRRLRLSLNALLPPDVAVLKA
                     APAAAGFVARDAASRSYRYDLWATTVKPVRERAYVWVLRGGVDEAALRAAAELLPGRR
                     DFAALTPSAHLYHHCVREVTAAAWTDDGPTPDGEGRRWRFEVSAGSFLHNMVRVAVGS
                     MVDVAQGRMSLEEFASALASGERRRMGQTAPARGLALVAVRYAGDGG"
     gene            complement(4022..4804)
                     /locus_tag="VFZ86_14930"
     CDS             complement(4022..4804)
                     /locus_tag="VFZ86_14930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEX5643609.1"
                     /translation="MRPAIAQYYPGDSVIHRLDARAKFVAVTALAVALFVRDSFAGLA
                     VFAAAGVIAYLLSGVPVAWFWRAFRPLLWLIALTFLAQVLFAPGEPFFSFWVFDFSWA
                     GLEFAAFLSLRLVVLVLIGSVLTLTTAPLALTDGLAWLGRPLRRLRVPTDELALMVTI
                     ALRFIPTLLVELDQIMRAQRARGADFSTGGPIRRARALVPVLTPLFVLSFRRADELAL
                     AMEARCYRPGIVRTRLRPLKAGAGDAVLLAVTAAVIAAGLLV"
     gene            complement(4801..5664)
                     /locus_tag="VFZ86_14935"
     CDS             complement(4801..5664)
                     /locus_tag="VFZ86_14935"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5643610.1"
                     /translation="MRDAAPAVDATSAGRGMALSCRDVAFSYEEARAPIPALRGVGFE
                     LAAGRSLALLGASGSGKSTLLQVVKGLDAPEAGAVLLDGTATGAPAYTGLQREVGLVF
                     QTPELQLFAVSAREDVAFGPRRLGWSEAEVAAAVDEAMELVGLPPADFGGRHPYALSG
                     GEQRRLALAGVLAMRPRLLLLDEPFVSLDPATRRELASILRRLRRDGVTLVLATHDVD
                     LAWALCDELLVLDAGRVAACGPWDLGESGRELLAASRLKEPFLVQLWRRLGRDPAGAP
                     RTVAEAAEALR"
     gene            complement(5661..6494)
                     /locus_tag="VFZ86_14940"
     CDS             complement(5661..6494)
                     /locus_tag="VFZ86_14940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5643611.1"
                     /translation="MPEALLQIRDLRYRYPGASVDALAGLDLTVSAGEFVAVVGANGS
                     GKSTLARILGGLEKPASAAVAVACGHDLLTEDGRMAARREVGVLFQNPENQLVAECVE
                     DDVAFGLENLAWPPADIRLRVDEMLARFWLTELRTREPHLLSGGQKQRTALAGVLAVP
                     RRVLVLDEPTAMLDPAGRAEVLDAVRGLRAEGLAVVYVTQEMDEVVGADRAVALDHGA
                     VVYSGAVRGLFGEGDLVRRLGLGLPAAGDLALELAARGRRLEPLPLTLDELVRDLGWT
                     P"
     gene            complement(6685..7035)
                     /gene="rplQ"
                     /locus_tag="VFZ86_14945"
     CDS             complement(6685..7035)
                     /gene="rplQ"
                     /locus_tag="VFZ86_14945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEX5643612.1"
                     /translation="MRHQNSGRKFNMAPSQRRSLLSGLACQVLEHEKIKTGVMRAKEV
                     RPMVEEAITLGKRGDIHARRQAIAMLRNKKIVYKLFNDVAPRYADRNGGYTRIIKLGP
                     RPGDGSEQAYIELV"
     gene            complement(7037..7984)
                     /locus_tag="VFZ86_14950"
     CDS             complement(7037..7984)
                     /locus_tag="VFZ86_14950"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003567511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEX5643613.1"
                     /translation="MLQFQMPNITREQLAGNRARFEVEPLDRGFGHTFGNALRRVLLS
                     SLEGAAVSSVKIEGVAHEFTTIKGVKEDVTDIILNLKELVCRLHGELDEVQVRLYKDK
                     PGKVVAADIDIPAELEILNPDHHIATLSDGGAIEMMLTIRRGTGYVAADGNKTPETVI
                     GVIPIDSIFSPIKRVSYHVESARVGQRTDYDKLILDITTNGALMPEEALREAAEILVD
                     RMSIFTDPDRTRSNEEFPIEGLTSAPAGGGMDDVLIEELELGVRSYNCLKREGIQTVG
                     DLVSKTEAELLNIPNFGKKSIDEVKDKLTERGLSLRGDE"
     gene            complement(8031..8651)
                     /gene="rpsD"
                     /locus_tag="VFZ86_14955"
     CDS             complement(8031..8651)
                     /gene="rpsD"
                     /locus_tag="VFZ86_14955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEX5643614.1"
                     /translation="MAKDTSPQCKQCRRESEKLFLKGERCLTDKCAVERRAYPPGEHG
                     RGRIKQSEYLLQLREKQKARRYYQVLEKQFHHYYVTASRKDGITGENLLRLLEMRLDN
                     VVYRLGFGASRRQARQLVRHAHFTVNGKKVDIPSYQVRPDDVVAIRASSKASETVRAA
                     TDLTSSVSPWLLVDHDNLTGKVLRAPERDEIDAPVQEQLIVELYSK"
     gene            complement(8662..9060)
                     /gene="rpsK"
                     /locus_tag="VFZ86_14960"
     CDS             complement(8662..9060)
                     /gene="rpsK"
                     /locus_tag="VFZ86_14960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEX5643615.1"
                     /translation="MAKPQRAKGRQRRKVRKNIAVGQAHIKTSFNNTQVTLTDKEGNV
                     IAWESAGSVGFKGSRKSTPFAAQVTAESCAKKGMEHGLQKVEVFVRGPGSGRETAVRS
                     LQAAGLEIISVKDVTPVPHNGCRPRKRRRV"
     gene            complement(9088..9459)
                     /gene="rpsM"
                     /locus_tag="VFZ86_14965"
     CDS             complement(9088..9459)
                     /gene="rpsM"
                     /locus_tag="VFZ86_14965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEX5643616.1"
                     /translation="MARIAGVNIPPQKRVEIGLTYIYGIGRSTSNKVLAIVGIDPNTK
                     VRDLTEEEIIKLRDAIDREVTVEGDLRRERSQNIKRLMDIGCYRGIRHRRGMPVNGQR
                     TRTNARSRKGPKRTISGKRKK"
     gene            complement(9466..9579)
                     /gene="rpmJ"
                     /locus_tag="VFZ86_14970"
     CDS             complement(9466..9579)
                     /gene="rpmJ"
                     /locus_tag="VFZ86_14970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006042225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEX5643617.1"
                     /translation="MKVRASVKQMCEKCKIIRRHGTVQVICSNPRHRQRQG"
     gene            complement(9656..9877)
                     /gene="infA"
                     /locus_tag="VFZ86_14975"
     CDS             complement(9656..9877)
                     /gene="infA"
                     /locus_tag="VFZ86_14975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEX5643618.1"
                     /translation="MANKEEAIEIEGEVVEALPNTMFRVHLDNNHEVLAHISGKMRMN
                     YIRILPGDRVKVELSPYDLSRGRITYRFK"
     gene            complement(9993..10766)
                     /gene="map"
                     /locus_tag="VFZ86_14980"
     CDS             complement(9993..10766)
                     /gene="map"
                     /locus_tag="VFZ86_14980"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015942767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEX5643619.1"
                     /translation="MIVRKSAGEIAKIAAAGAILADCIDALTEAAAPGVTTAELDRLA
                     EKFIRRHRGVPTFLGYRGFPASICASPNDMVVHGIPGKYRAAEGDLLSIDVGVTLEGY
                     VADSAVTLPMGAVEPEAQRLLDVTRESLEAGIAECREGRRLGDISHAVQEVVEAAGFS
                     VVRSLVGHGVGKEMHEDPQIPNYGDPGRGPRLEEGMVFAIEPMVDAGAYEVVVADDGW
                     AIHTADGSLSAHFEHTVAVGKKGPQVLTRRRSERAGAGV"
     gene            complement(10763..11422)
                     /locus_tag="VFZ86_14985"
     CDS             complement(10763..11422)
                     /locus_tag="VFZ86_14985"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014969163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEX5643620.1"
                     /translation="MTQRNVILLGGPGAGKGTQAERIVADFALPHISTGEMLRAAVAK
                     ATGMGREAQTYMERGALVPDEVVIGVVRDRLAEPDAAEGFLLDGFPRTVPQAEALDAL
                     LAEGGRAITHVVAIDVPADELVQRIAGRRSCAVCGKIYNVVFDPPRADGVCDLDGGEL
                     VQRADDNEETVRKRIGVYERQTAPLVGYYTDKGVLQSAFGGGKRPDEVYEQVQRILAG
                     A"
     gene            complement(11431..12696)
                     /gene="secY"
                     /locus_tag="VFZ86_14990"
     CDS             complement(11431..12696)
                     /gene="secY"
                     /locus_tag="VFZ86_14990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEX5643621.1"
                     /translation="MNNKLWQSLANAWRIPELRTKLLFTAGMVAIYRFGSFVPVPGID
                     LEALKQFFEEGGAGVVGFLDLFSGGALSRVAIFALGIMPYITASIIMQLLTVVIPKLE
                     ELAKEGEQGQKQITKYTRWLTVGLALVQSLGYIVLFRQYNALPDLNWWRGALIVTSLV
                     AGAVVVMWLGELITARGIGNGMSIMIFISIVSSIPGGAAQLFTMSLVAIVGFMVIALA
                     VVVGVIWITTGERRIPVQYAKRVVGRRMMGGQSTFIPLKVNMAGVIPVIFASSVMMII
                     PTFAQFLGTDGIGGWFQRVLGPSTFLFITFEAILIVIFTYFYTAVTFNPIDQADNLKK
                     YGGFIPGVRPGRPTAEYLDRILTRLTFPGALFLAALAVLPWLLSRPPVNVPFYFGGTS
                     LLIVVGVALDTMRQMEAQLLMRHYEGFLK"
     gene            complement(12716..13183)
                     /gene="rplO"
                     /locus_tag="VFZ86_14995"
     CDS             complement(12716..13183)
                     /gene="rplO"
                     /locus_tag="VFZ86_14995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEX5643622.1"
                     /translation="MGLHNLRPNPGSTKATKRLGRGKGSGQGKTAGKGHKGYNARSGG
                     GVRPGYEGGQMPLYMRLGKLRGPHMKKSMPIGPFRTYAVPVNVGRLANAFAAGDEVTP
                     EALVAKGIVKNTRTPIKVLGGGELTVALTVRVHLYSRSAVEKIEAAGGKAELL"
     gene            complement(13197..13391)
                     /gene="rpmD"
                     /locus_tag="VFZ86_15000"
     CDS             complement(13197..13391)
                     /gene="rpmD"
                     /locus_tag="VFZ86_15000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEX5643623.1"
                     /translation="MADYIEITQTKSAIGRLPEHRGTLRALGLKRIRHTVVHEDTPVI
                     RGMVNHVAFLVEVRPAAKKS"
     gene            complement(13397..13954)
                     /gene="rpsE"
                     /locus_tag="VFZ86_15005"
     CDS             complement(13397..13954)
                     /gene="rpsE"
                     /locus_tag="VFZ86_15005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEX5643624.1"
                     /translation="MARGSFSEGNELKERVVDINRVAKVVKGGRRFSFTALVVVGDEN
                     GHVGAGYGKAREVPLAIQKAIEDAKKNLFTVPRYNGTTTTHQVIGRYGAARVLLKPAA
                     PGTGVIAAGGVRAVLELAGVRDILTKSLGTTNPINLVRATVAGLQSLKTPEGQARLRG
                     ISVAQVLGLDQRKPEAAEAADVKEA"
     gene            complement(13973..14326)
                     /gene="rplR"
                     /locus_tag="VFZ86_15010"
     CDS             complement(13973..14326)
                     /gene="rplR"
                     /locus_tag="VFZ86_15010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEX5643625.1"
                     /translation="MSATSIREARARRKRRIRGRISGTTERPRVSVFRSNKAIYAQLV
                     DDAAAATIVAARSSEVDGSGLKKAEVAKKVGELLAQRAKDKGIERVVFDRSGYLYHGR
                     VKALAEGAREGGLVF"
     gene            complement(14358..14891)
                     /gene="rplF"
                     /locus_tag="VFZ86_15015"
     CDS             complement(14358..14891)
                     /gene="rplF"
                     /locus_tag="VFZ86_15015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEX5643626.1"
                     /translation="MSRIGKAPIPLPGSVEFKYDNAVVTVKGPKGELTQRVPREMMVE
                     VGDGVIQVKRPTDSGQHRSLHGLTRTLIANMVTGVTDGFEKRLEIVGVGYRASIKGDA
                     LELLVGYSHPVVIEPPEHVTFETPQQTVVVVRGIDKQVVGEVAAKVRSIRPPEPYKGK
                     GIRYAGEYVQRKVGKRA"
     gene            complement(14901..15299)
                     /gene="rpsH"
                     /locus_tag="VFZ86_15020"
     CDS             complement(14901..15299)
                     /gene="rpsH"
                     /locus_tag="VFZ86_15020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEX5643627.1"
                     /translation="MSMTDPIADMLTRIRNGSTARHSTVEIPASRMKAEIARLMTEQG
                     YIAGYDTKQGEHGQVIVVELKYGKNRERVISGIKRVSKPGRRVYAKQETLPKVLGGLG
                     TAIISTSRGLVTSAEARKLGLGGEVICFIW"
     gene            complement(15313..15498)
                     /locus_tag="VFZ86_15025"
     CDS             complement(15313..15498)
                     /locus_tag="VFZ86_15025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003948630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEX5643628.1"
                     /translation="MAKTSLVVKAGRKQRYQTRQYNRCQRCGRPRAYYRKFGLCRICL
                     REVAHQGVIPGLTKSSW"
     gene            complement(15512..16054)
                     /gene="rplE"
                     /locus_tag="VFZ86_15030"
     CDS             complement(15512..16054)
                     /gene="rplE"
                     /locus_tag="VFZ86_15030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEX5643629.1"
                     /translation="MARLKDRYFKEIVPELKDEFGYRNVMEVPRVTKVTLNMGVGEAK
                     TNAKLLDDAVEELALITGQRPAITKARKSIAGFKLREGMSIGCRVTLRGERMYEMIDR
                     LVSIALPRIRDFRGIDPGQFDGRGNFAMGVREQTIFPEIDYDSVETFRGLNVVITTTA
                     KTDEEGRALLAKLGMPFRSN"
     gene            complement(16059..16385)
                     /gene="rplX"
                     /locus_tag="VFZ86_15035"
     CDS             complement(16059..16385)
                     /gene="rplX"
                     /locus_tag="VFZ86_15035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008195011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEX5643630.1"
                     /translation="MKIKKGDQVRVISGKEAGKTAKVSRVDTEKRRVLLERLNLVKKH
                     TKPNPKTNPQGGVIEREAPVDASNVMLVCPSCGQPTRVGYRLTEGGGKVRTCRRASCG
                     KDIDKA"
     gene            complement(16394..16762)
                     /gene="rplN"
                     /locus_tag="VFZ86_15040"
     CDS             complement(16394..16762)
                     /gene="rplN"
                     /locus_tag="VFZ86_15040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEX5643631.1"
                     /translation="MIQVETRLRVADNTGAREILCIRIRGGSKRRYGTVGDIIVGTVK
                     VATPGGSVKKGDVVRAVIVRTKKEYGRKDGTYIAFDENAAVLIDNQNNPRGTRIFGPV
                     ARELREKNFMKIVSLAPEVL"
     gene            complement(16784..17239)
                     /gene="rpsQ"
                     /locus_tag="VFZ86_15045"
     CDS             complement(16784..17239)
                     /gene="rpsQ"
                     /locus_tag="VFZ86_15045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014000372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEX5643632.1"
                     /translation="MATTKKTDSGKPAAKQSAARKPAAKKAAAKKAAPKKTTAKKAVA
                     PEAKPAKKVSAARSKAKAERAAPEKVPGRKERRGIVVSDAMDKTIVVRIDVAQKHERY
                     GKVVRRSSKLHAHDEQNAAGVGDLVRVVETRPLSATKRWRLLEVLEKAK"
     gene            complement(17239..17451)
                     /gene="rpmC"
                     /locus_tag="VFZ86_15050"
     CDS             complement(17239..17451)
                     /gene="rpmC"
                     /locus_tag="VFZ86_15050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEX5643633.1"
                     /translation="MKASKLRELSDDELIVRLKDTRKELFNLRFQHATGQLDNPMKLN
                     ATRREIARLLTMQAEREHERTAGGEA"
     gene            complement(17441..17866)
                     /gene="rplP"
                     /locus_tag="VFZ86_15055"
     CDS             complement(17441..17866)
                     /gene="rplP"
                     /locus_tag="VFZ86_15055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007505920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEX5643634.1"
                     /translation="MLLPKRVKHRKQQRGRMAGEAKGGTTVEFGQYGLKAIERGWITN
                     RQIEAARIAMTRYIKRGGKVWINVFPHKPVTRKAAETRMGGGKGSPESWVAVVKPGKV
                     MFELAGVSEPVAREAMRLAAHKLPIKCKFVTREGSLFEG"
     gene            complement(17885..18835)
                     /gene="rpsC"
                     /locus_tag="VFZ86_15060"
     CDS             complement(17885..18835)
                     /gene="rpsC"
                     /locus_tag="VFZ86_15060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEX5643635.1"
                     /translation="MGQKVHPGGFRVGVIHDWKSHWYTEKEFKQFLAEDERIRLHILR
                     KLSHAGLSRIIIRKDQNNITIDIHTARPGIVIGKSGSEVDALRKEIHAMTRKNVQVNI
                     IEVKRPELDAVLVAQSIAEQLENRVSFRRAMKRALTSAMRSGAAGMRVRCGGRLGGAE
                     MARNEHYSEGRVPLHTLRADIDYGFREARTTFGRIGVKVWINKGEILPEGYPMDEAKA
                     RMERDEQRRDDRGGRDRRPRRDGAPGGRGPGGPGRGPGGPGRGPGGPGRGGQNRGPGG
                     QRPSVGPKAPMGRQAPNRSAKPADAPKPETKPDAPKTEQS"
     gene            complement(18835..19179)
                     /gene="rplV"
                     /locus_tag="VFZ86_15065"
     CDS             complement(18835..19179)
                     /gene="rplV"
                     /locus_tag="VFZ86_15065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEX5643636.1"
                     /translation="MAVRAQAKYVRVAPRKAREAADLIRGKSVAEARSVLFFANRNSA
                     RLVAAVLDSAVANAEHNNALSADDLYVKEARVDEGPTLKRWQFRAMGRVNRIRKRSSH
                     ITVAVEQREGRS"
     gene            complement(19186..19464)
                     /gene="rpsS"
                     /locus_tag="VFZ86_15070"
     CDS             complement(19186..19464)
                     /gene="rpsS"
                     /locus_tag="VFZ86_15070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEX5643637.1"
                     /translation="MSRSAKKGPFVDERLMKRIEQMNAAGQKKMIKTWSRASTVFPDM
                     VGHTIAVHDGRKHVPIFVSESMVGHKLGEFALTRTFRGHIKGDRSARR"
     gene            complement(19482..20321)
                     /gene="rplB"
                     /locus_tag="VFZ86_15075"
     CDS             complement(19482..20321)
                     /gene="rplB"
                     /locus_tag="VFZ86_15075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEX5643638.1"
                     /translation="MGIKRFKPTSPGRRFMTVDTFEDITRAEPERSLVAPLKKNGGRN
                     SYGRITTRHKGGGHKRRYRIIDFKRDKDGVPAKVAHIEYDPNRSARIALLHYADGEKR
                     YILAPKALVVGDTVESGPGADIKAGNALPLENIPSGTTIHNIELQPGRGGQLVRSAGT
                     SAQLQAKEGGHGVVRLPSGELRRIRLTCRATVGELGNSDHSNLSGGKAGRTRWRGVRP
                     TVRGTAMNPVDHPHGGGEGKTTAGRHPVTPWGVPTLGYRTRAKHKASDKLIIRGRKRG
                     KRG"
     gene            complement(20321..20608)
                     /gene="rplW"
                     /locus_tag="VFZ86_15080"
     CDS             complement(20321..20608)
                     /gene="rplW"
                     /locus_tag="VFZ86_15080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEX5643639.1"
                     /translation="MVEAEKIIIRPIISEKSYAQIDHSRYTFEVHPDAHKTMIAQAIQ
                     QLFNVTVVGVSTANVRPKPKRRGLSKGRTSAWKKAVVQLAPGDKIEFFEGR"
     gene            complement(20612..21640)
                     /gene="rplD"
                     /locus_tag="VFZ86_15085"
     CDS             complement(20612..21640)
                     /gene="rplD"
                     /locus_tag="VFZ86_15085"
                     /inference="COORDINATES: protein motif:HMM:TIGR03953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEX5643640.1"
                     /translation="MSADEIKKTPDVEAAAEPEKTEAEAAGKTEAEAPKKKPAARKPA
                     AKKPAADKADDATADEKAPARKPAAKKPAAKAEAADEKAADEKAAKPAAKKAAAKKPA
                     AKKPAAKAAEKPAPKAERPPKTAPAAKAAAEARKGAAGVATFVDAAGKEVETRKLTDV
                     RFAVAADVNTLHLVVRAEQAARRSGTASTKTRGEVTGSTAKLYRQKGTGRARAGSVKS
                     PIRTGGGVVFGPRPRSFDIKVNKKVARKALAMALSDRAANGRVFVAAGLEMSEPSTRT
                     LEEFLVNLDCAAPVLVVTHDEPVVTRSVRNLPYAEASEVAALSTEQVLRARSLVLTEK
                     AFAALNEA"
     gene            complement(21643..22257)
                     /gene="rplC"
                     /locus_tag="VFZ86_15090"
     CDS             complement(21643..22257)
                     /gene="rplC"
                     /locus_tag="VFZ86_15090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEX5643641.1"
                     /translation="MTGIIGKKMGMTQLFDEDGNVIAVTVVQAGPCPVTQVRTLDNDG
                     YEAVQLAFEECKDKHITKPELGHLKKAGLKHGMRHLVEFDGVGDLKVGDTVTVEAFED
                     GQAVTATGVSKGKGFQGTIKRHNFGRGPETHGSHNVRQPGSIGASAYPSRVFKGMRMS
                     GHMGAQRVTQQGLKIVRRDVENNLLLIKGSVPGAKNSVVVIKGR"
     gene            complement(22262..22570)
                     /gene="rpsJ"
                     /locus_tag="VFZ86_15095"
     CDS             complement(22262..22570)
                     /gene="rpsJ"
                     /locus_tag="VFZ86_15095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012084982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEX5643642.1"
                     /translation="MAQQKIRIRLKSYDHELIERSAQSIVETAQRSGATISGPVPLPT
                     EKNVYCVIKSPFKDKDSREHFEIRTHKRLIDIHQPTPKTVDQLMRLDLPAGVHIEIKL
                     "
     gene            complement(22661..23866)
                     /gene="tuf"
                     /locus_tag="VFZ86_15100"
     CDS             complement(22661..23866)
                     /gene="tuf"
                     /locus_tag="VFZ86_15100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEX5643643.1"
                     /translation="MAKQKFDRSKPHMNVGTIGHVDHGKTTLTAAITLVLHNRGGNTE
                     FTAFDMIDKAPEERERGITIATAHVEYETEKRHYAHVDCPGHADYIKNMITGAAQMDA
                     AILVVGADDGPMPQTREHILLAHQVNVPNIVVYMNKTDLVDDPELIELVEMEVRELLS
                     EYEFPGDDLPVIKGSALKALECGCGKDDCEWCGSIIELTNALDEYLPEPERDIDKPFL
                     MPVEDVFTITGRGTVATGRVERGIVKVGEEVEIVGMRDKPMKTTVTGVEMFRKLLDEG
                     RAGDNIGLLLRGVKREEVERGMVCSKPGSITGHTHFLTQVYVLSKDEGGRHTPFFNGY
                     RPQFYFRTTDVTGVITLEEGVEMVMPGDNTVMEVKLITPIGMEEGLRFAIREGGRTVG
                     AGAITKIIA"
     gene            complement(24000..26072)
                     /gene="fusA"
                     /locus_tag="VFZ86_15105"
     CDS             complement(24000..26072)
                     /gene="fusA"
                     /locus_tag="VFZ86_15105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX5643644.1"
                     /translation="MRQYSLDNTRNIGIMAHIDAGKTTTTERILYYTGKTHKIGEVHE
                     GAAVMDWMAQEQERGITITSAATTVSWRDTQINIIDTPGHVDFTMEVERSLRVLDGAI
                     ALFCSVGGVEPQSETVWRQADKYGIPRIAFVNKMDRIGADFYRGLEMMVDRLGANPVA
                     LQIPIGREAEFRGIIDLIEMNATIYLDELGTDFEVTDIPDDHRELADEYRLKLVESIA
                     DHDDHLMEAYLEGEEITPGHLREVIRQATLDISITPVMCGSAFKNKGVQPLMDAIVDF
                     LPSPLDVPAVDGTKPNGEVVTRQTADDAPFSALAFKVAVDPYVGKLTYFRVYSGTLKA
                     GSYVLNSSRDKRERISRILQMHANHREDRDVIYAGELAAAVGLKVTGTGDTLCDPSKP
                     VILESMEFPEPVIQVAIEPKTKADEEKLGNSLQRLAEEDPTFRVHTDEETGQTIIAGM
                     GELHLEIIVDRLVREFGVDANVGRPQVAYRETIRNKVEKVEGRFVRQSGGRGQYGHVR
                     IDMAPNEPGGGYEFESKIMGGVVPRDYIPSVDDGIQEAMSTGVLAGYPMVDIKVTLVD
                     GSYHEVDSSDMAFKIAGSLAFKNAAQKAKPVLLEPMMAVEVVTPADFMGDVMGDINSR
                     RGHIDGMEPRGNAQVINATVPLSTMFGYATDLRSSTQGRATYTMQFKHYAEVPASIAT
                     EIVAKVRG"
     gene            complement(26142..26612)
                     /gene="rpsG"
                     /locus_tag="VFZ86_15110"
     CDS             complement(26142..26612)
                     /gene="rpsG"
                     /locus_tag="VFZ86_15110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEX5643645.1"
                     /translation="MPRRGTITRREVLPDPVYNSKLVTQVINKVLLDGKKSTAEQIVY
                     GALEIVREKTGRDPVEVLQDAIQALTPHLEVRSRRVGGATYQVPVEVPGRRARTLAIR
                     WLVSFARDRREKVMAERLAAEMLDALAGQGGAFKKKDDIFRMAQANKAFAHYRW"
     gene            complement(26620..26991)
                     /gene="rpsL"
                     /locus_tag="VFZ86_15115"
     CDS             complement(26620..26991)
                     /gene="rpsL"
                     /locus_tag="VFZ86_15115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007514810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEX5643646.1"
                     /translation="MTTISQLVRKGRESKSKRSKTPALKGSPQKRGVCTRVYTVTPKK
                     PNSALRKVARVRLVNGMEVTTYIPGIGHNLQEHSVVLVRGGRVKDLPGVRYKVIRGSL
                     DASGVADRKKARSRYGSKAKG"
     gene            complement(27123..>29903)
                     /locus_tag="VFZ86_15120"
     CDS             complement(27123..>29903)
                     /locus_tag="VFZ86_15120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011435033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643647.1"
                     /translation="PKMMALELFKPFIMSRLVGRKMVQNIKAAKKMVESMVPEVWDIL
                     EEVITEHPVMLNRAPTLHRLGIQAFEPLLVEGKAIQIHPLVCAAFNADFDGDQMAVHL
                     PLSWEAQAEARVLMLSTNNILSPAHGKPIAVPSQDMVIGVYYLTYHAEDDLSEAQPDH
                     FGEQLHAYSGYEEVQRAWEQRILDQREVPTKRSAGIAEFTRSPILLRLTTGERVVTTV
                     GRALFNMEVEEGLREVLGEEYAGDYTFINYTMTKKGLAAFINKLVALYGANKVARILD
                     VFKEVGFHFATRAAVTVSKNDVVVPAKEKAEILGTHEAMVDQLRDEYNNGFLSEEERR
                     GKVQEIWREADVQITAATRANFNPLNPIYMMATSGARGDIKQIRQLAGWRGQMANPKG
                     EIVEQPVKSSFIEGLSVLEFFISTHGARKGLADTALRTADSGYLTRRLVDVCQDVTVS
                     DIDCGTKEWVPMSPWSRRREENPGLDGAVLAEDVRRPRSDRIVLKKGEKIGPAQIGLL
                     REVYSMLPNAKVKVKKGKAAAAMMAVWTHEPNENLLGRHLGDAIVEEESGEVVFDRDT
                     HIDVATRDQILDILARSSQTDPMVPVRSVLKCDAEIGVCQKCYGYALANNAPAEIGDA
                     VGHVAAQSIGEPGTQLTMRTFHTGGIAGADITHGLPRVVELFEARKPKGVARIAEVTG
                     RVSIEDTERGCKVTITPDEGAAKEYTIAARRTHVMVEDDDWVEEGTQITPGSLYPADL
                     LKHAGATATEKYLVNEVQEVYRSQGVEINDKHIELIVRRMMRKVRIVTAGDTTFLPGQ
                     LVDRYQFVAENERMSAEGAEAAEAEEIILGITKASLATESFLSAASFQETTKVLTDAA
                     IEGKVDNLAGLKENIIIGKLIPAGTGLRRYRGVEIHAAGRAEALAEADRLMTGDPAWS
                     LGDGDGLDGEIE"
BASE COUNT         4112 a        10176 c        10436 g         5179 t
ORIGIN      
        1 ccgctggccg gccaagcacg acgtcatctt ctggtacgcc cgcgacccgg cccggtacgt
       61 gttccgctat gacgacatcg accgggtgcc gtacctggcg cccggcctcg tgggtccgga
      121 gaaggccgct cgcggtatga cgccgacgga cgtgtggtgg cagaccatcg tcagccccac
      181 gggagcggag aagaccggct acccgacgca gaagccggtc aagatcctcg agcgcatcgt
      241 gcgcgtgcac tccgacccgg gcgacctggt cgtggacccg ttcgccggca gcggcacggc
      301 cggagaggcg gcggcgcggc tcgggcgcgg ctacctgctg gtggacgaga acccggaggc
      361 ggtgcgcgtg atgcgcgagc gcctggcgtg ggacgggccg gagaccgtct cgggcaccgg
      421 gccggccggc ggatccgcct cgtcgccggc gccgccggcc gagcctggcc cctgagacgc
      481 gacggggccg gctcctcgcg gagccggccc cggtgacgtc acaagcgccg gagtgggatc
      541 actcaggcac gatcgcgtcg ctcgggcagg actcgacgca ggtcgcgcag tcgtcgcact
      601 tctcgggatc gatgacgtag atgtcgtcgc cctcggagat ggcctcgttg gggcactcgt
      661 cgatgcaggc gccgcaagca aggcattcat cggtgatctt gtaggccatg tggtggtgtc
      721 acctcctgta ggcgcgccgc gaacacggta cgcgcgggat tccagggtcc atttacgggc
      781 atggcggagg cgaagtcaag aacgcctcca gcgcccgcgt cagcccgcgt cgccggggaa
      841 cagctccgtg agcgccaggg ccgcggcgcc gatgaatccg gcgtccgggc ccagcgccgc
      901 cggcacgacg cgtacgcggt cgcgcgccgg cggcagagcc cgcgcggcga ggacgcggcg
      961 ggcgggagcg aggagcagct cgcccgccgc cgcggcgccg cccccgatga cgatgagctc
     1021 ggggtcgaag atgttggaga gcgtgaccag gccggcgccg aggtactcgc cggcgcgctc
     1081 cagcacggcg atcgcgccgt cgtcgccctc gcgcgccagc tgcgtcagga gacggctgtc
     1141 cacggtgcgg ccctcggtga gggccctgcc cagggccgag tcgggcgcgc gcccggcggc
     1201 cgcccgggcg gccgcgccca tggcgggtcc ggccacgtac gcctcgaggc aaccgtgatt
     1261 ggggcagttg gcggggcact tcggcccgtc gacgtcgatg atcatgtggc cgatctccgc
     1321 ggccgcgccg ctggcgccgc gcaggggacg gccgccgctg atgatgccgc cgcccacacc
     1381 ggtgccgagg gtgagcatga gcatgtcgct ggtgccggcg ccggcgccgt acgcgtgctc
     1441 gccgatggcg gcggcggtgg cgtcgttgtc cacgacgacc gggacgccga agcgccgctc
     1501 gaggtggtcg cgcagcggga cgtcggtgag cggcaggttg accgattcga cgacgcgccc
     1561 gccggcgaag tcgaccatgg aggcgactcc gacgccgatg ccctccaccc ggccggagcc
     1621 gtgctccagg tccgcgaggc aggcgtcgat gccgcgcagg caggcctcgg cggagctcac
     1681 atcggtgagc gtggtgaacc ggtcgaggat ctccggcgtc tccaccccgg agcgtgagcg
     1741 cgctccgggc gcgggggccc gccctcgcac gagcgcggcg gccactttgg tgccgccgat
     1801 gtccacgccg acgacgatgc gctcgggagc catgacgtcc cgagcttacc atggtgtaga
     1861 ctcgggacta gaggaaacgc ccgctcacgg gcgtagtctg caacggcacc cggcttcccc
     1921 gccccccgag accacgcgta tcgatgtcgc ctgcgtccgc gggccggagg cgccaccaca
     1981 tggcgagaca ggacgaggac agagaagggg caaagcccta caagacgtac aaggccggcc
     2041 gcggcaggcg cagccagctc gacgacgaac tcgcaggcgc acggccggcg cgcacgcgac
     2101 ccgatggcga cgccgaccgc ggccccgcct accctcgcca gtccgacaag gcgtaccaca
     2161 cgtacgggcc cgcgccggcc gacggacgcg cggccggcac gaacaagagc agaggcgccg
     2221 gagccggtcc ccgccgccgg cgccgcttcc gctggtggta cgtccccgtc ggcgtcttcg
     2281 ccctgctggt catcgccggc gtcgccgcta ccgtcctcgc gtggcccggg taccagaagt
     2341 tcgaccgggc cgtcgacaag gccaacaagc gcgtggacgg gcagactcgc gcccagctcg
     2401 ccgcggacga cggctggctc tggcgcaacg gcaccacggt ggcgctgttc ggcctggacg
     2461 aggccggcct gccggcgcac tcggacacca tcctgctcat gcacttcgac gcgaagtcac
     2521 acaccatcaa ccagctctcg atcccgcgcg acacgctggt gaacgtcgag gggtacggcc
     2581 cgcagaagat cacgcaggcg atgtggtacg gcgggccgtc cctggcgctc aagacactca
     2641 aggagtacac cggcatcccc atcaaccaca tcatggtcgt gagcttccag ggcttccccc
     2701 ggctcgtgaa ctccgtcggc ggcatcgaca tgtacgtacc gcagacggtc cagaccgagg
     2761 cgggctcgaa ccgccgcctg gtcaccttca agaagggcat gcaccacttc gacggcaaga
     2821 acgcgatgct gtacgtgcgc atccgcaagg cgtacgccga gggcgacttc acgcgcgcca
     2881 agcgccagca ggcgttcgtc gacgcgctcc agaagaagat cgtcaagccg tccaacatca
     2941 ccaggctgcc cgatatcggc cgtcacttca tgagtggcgt ggccaccgac ctcaccacca
     3001 accagatcct ggaactggcc tacctcaagt ggcgcgccgg cggcggcaag aagcaggtgc
     3061 tgaagggaga gctcggctgg agcgacgacg gccaggcagt ggtgctgccg cccagcgacg
     3121 ccgcccggca gaaggccgtc gccaggttcc tcggggagtg acgccgccgc ggccctcgcc
     3181 ggccgcgccg gcgccggacg cgtctccccc cgctcagccc ccgtcgcccg cgtagcgcac
     3241 ggcgaccagc gcgagccccc gagcgggcgc ggtctgaccc atgcggcggc gctcaccgga
     3301 ggccagcgcc gacgcgaact cctccaggct catgcgtccc tgggccacgt cgaccatgga
     3361 gccgacggcc acgcggacca tgttgtggag aaagctgccg gcgctgacct cgaagcgcca
     3421 gcgccggccc tccccgtccg gcgtcgggcc gtcgtcggtc cacgccgcgg ccgtcacctc
     3481 gcgcacgcag tggtgataga ggtgcgcgga gggcgtgagc gcggcgaagt cgcggcggcc
     3541 cgggagcagc tccgccgccg cccgcaacgc cgcctcatcc acgccgccgc gcagcaccca
     3601 cacgtaggcg cgctcgcgca cgggcttgac cgtggtcgcc cagaggtcgt accggtagct
     3661 tcgggaggcc gcatcgcgag ccacgaaccc ggccgcggcc ggcgccgcct tgagcacggc
     3721 gacgtcaggc ggcaggagcg cgttcaggga gaggcgcaga cgccggggcg agaggtccga
     3781 gcgggcgcag gtgaacgacg ccacctgcgc ccaggcgtgc acgcccgcgt ccgtgcgccc
     3841 ggccacgctg aggcggacgc gctcgcgcag caccgtctcg agcgcgtccc cgagcaggct
     3901 ctctaccgtc gtctgacccg gctgcctggc ccagccgcga tacctcgtgc cctcgtaggc
     3961 gaggtcgagc cgccaggtcg tcccgccgga ggtctcctga ctgccgtcca cgcccggcat
     4021 cctacacgag cagcccggct gcgatgacgg ccgcggtgac ggccagcaga acggcgtcgc
     4081 cggcccccgc cttgagcggc cgcaggcgcg tgcgcacgat gccgggccgg tagcagcgcg
     4141 cctccatggc gagcgccagc tcgtcggcgc gccggaagct cagcacgaac agcggcgtga
     4201 gcaccgggac cagcgctctc gcccgccgga tgggaccgcc ggtggagaag tcggcccccc
     4261 tggctcgctg ggcgcgcatg atctggtcca gctcgaccag cagcgtcggg atgaagcgca
     4321 gcgcgatggt caccatgagc gcgagctcgt cggtcggcac gcgcagccgg cggagcggcc
     4381 gccccagcca cgcgagcccg tcggtcagcg ccagcggagc cgtggtcagc gtgagcaccg
     4441 acccgatgag caccagcacc acgagccgca ggctgaggaa ggcggcgaac tccagccccg
     4501 cccaggagaa gtcgaagacc cagaagctga agaacggctc gccgggagcg aacagcacct
     4561 gggcgaggaa cgtgagcgcg atcagccaga ggagcggccg gaacgcgcgc cagaaccagg
     4621 cgacgggcac gccgctcagc aggtacgcga tcacgccggc cgcggcgaac acggccagac
     4681 cggcgaacga gtcgcgcacg aacagcgcga ccgcgagcgc cgtcacggcc acgaacttgg
     4741 cgcgcgcgtc gagccggtgg atgacggaat cgcccgggta gtactgcgcg atcgccggcc
     4801 tcatcgcagc gcctccgcgg cctcggcgac ggtgcgcggc gccccggccg ggtcgcgacc
     4861 gaggcggcgc cagagctgca cgaggaaggg ttccttcagc cggctggcgg cgagcaactc
     4921 gcggccgctt tcgccgaggt cccaggggcc gcacgccgcg acgcgcccgg cgtcgagcac
     4981 cagcaactcg tcgcagagcg cccaggcgag gtcgacgtcg tgggtggcga gcacgagcgt
     5041 cacgccgtcc cggcgcaggc gccgcaggat gctcgcgagc tcgcggcgcg tcgccggatc
     5101 caggctcacg aacggctcgt cgagcaggag aaggcgcggc cgcatcgcga gcacgccggc
     5161 gagcgcgagg cggcgctgct caccgccgga gagggcgtag gggtgccgcc cgccgaagtc
     5221 tgccggcggc agcccgacca gctccatcgc ctcgtcgacg gcggccgcga cctccgcctc
     5281 ggaccagccg aggcggcgcg ggccgaacgc cacgtcctcc cgcgccgaca ccgcgaacag
     5341 ctgcagctcc ggcgtctgga acacgaggcc gacctcgcgc tggagacccg tgtaggcggg
     5401 cgccccggtg gccgtgccgt ccagcagcac ggcgcccgcc tccggcgcgt cgaggccctt
     5461 caccacctgc aggagcgtgg acttgcccga cccggacgcg ccgaggagcg ccagggaccg
     5521 gccggcggcg agctcgaagc cgacgccgcg cagggcgggg atcggcgcgc gcgcctcctc
     5581 gtagctgaag gcgacgtcac ggcaggagag cgccatgccg cgccccgcgc tcgtcgcgtc
     5641 cactgcaggt gcggcgtccc tcacggggtc caccccaggt cgcgcacgag ctcgtccagg
     5701 gtcaggggca gcggctcgag ccggcgcccg cgggctgcga gctcgagcgc caggtcgccg
     5761 gccgcgggca gccccagccc cagacgccgg accaggtcgc cctcgccgaa caggccgcgc
     5821 acggcgccgg agtacacgac ggcgccgtgg tcgagcgcga cggcccggtc ggcacccacg
     5881 acctcgtcca tctcctgggt cacgtagacc accgcgaggc cctcggcgcg caggccgcgg
     5941 accgcgtcca ggacctcggc gcggccggcg ggatccagca tggccgtggg ttcgtcgagc
     6001 acgaggacgc gccgcggcac ggccaggacg ccggcgagcg cggtccgctg cttctgcccc
     6061 ccggagagca ggtgcggctc ccgcgtgcgc agctccgtga gccagaaccg cgccagcatc
     6121 tcgtccacgc gcagccggat gtcggcgggc ggccacgcga ggttctcgag gccgaaggcg
     6181 acgtcgtcct cgacgcactc cgccaccagc tggttctcgg ggttctggaa cagcacgccg
     6241 acctcgcggc gcgcggccat gcggccgtcc tcggtcagga ggtcgtggcc gcaggcgacg
     6301 gcgaccgcgg cggaggccgg cttctccagg ccgccgagga tgcgcgcgag ggtggacttg
     6361 ccggagccgt tggcgccgac gacggcgacg aactcgcccg cggacaccgt gaggtcgagc
     6421 cccgccagcg cgtcgacgga cgcgcccggg taccggtagc gcaggtcgcg gatctggagg
     6481 agggcttcag gcacgtcgcg ggcaggagcg gcagagggcg tcatgaccgc cgaagcttac
     6541 cgcatcgccg caggcgtccc cgggagctgc gtccgacgcc cgcgccgggc tgccgccgga
     6601 cgccgtcgtc cggctccgga aacgacgagg ggcgggaggc ccttcggccg cccgcccctc
     6661 gtccaccact tctgccgccg gccgtcagac cagctcgatg tacgcctgct cgctgccgtc
     6721 gccggggcgc ggcccgagct tgatgatgcg cgtgtagccg ccgttgcggt cggcgtagcg
     6781 cggcgcgaca tcgttgaaca gcttgtagac gatcttcttg ttgcgcagca tggcgatcgc
     6841 ctgccggcgc gcgtggatgt cgccgcgctt gccgagcgtg atggcttctt cgaccatcgg
     6901 acgcacctct ttggcgcgca tcacgccggt cttgatcttc tcgtgctcga gcacctgaca
     6961 ggccaggccc gagagcagcg agcggcgctg gctcggggcc atgttgaact tacggcctga
     7021 gttctgatgg cgcatgttac tcgtcacccc gcagagagag cccgcgctcg gtgagcttgt
     7081 ccttgacctc gtcgatgctc ttcttgccga agttggggat gttgagcagc tcggcctctg
     7141 tcttggagac caggtcgccg acggtctgga taccctcgcg cttgaggcag ttgtacgaac
     7201 ggacgccgag ctcgagctct tcgatgagca cgtcgtccat gccgcccccg gccggcgcgg
     7261 aggtgagtcc ctcgatgggg aactcctcgt tgctgcgcgt gcggtcgggg tcggtgaaga
     7321 tcgacatccg gtcgacgagg atctcggcgg cctcgcggag cgcttcctcg ggcatgaggg
     7381 cgccgttcgt cgtgatgtcg aggatgagct tgtcgtagtc ggtgcgctgg cccacgcggg
     7441 cgctctcgac gtgataggag acgcgcttga tggggctgaa gatggagtcg atcgggatga
     7501 cgccgatgac cgtctccggg gtcttgttgc cgtcggcggc cacgtagccc gtgccccgcc
     7561 ggatggtgag catcatctcg atggcgccgc cgtcggacag cgtcgcgatg tggtggtccg
     7621 ggttgaggat ctccagctcg gccgggatgt cgatatcggc cgcgacgacc ttaccgggct
     7681 tgtccttgta cagccgcacc tggacctcgt cgagctcgcc gtgcaggcgg cagaccagtt
     7741 ccttgaggtt gaggatgatg tcggtgacgt cttccttcac gcccttgatg gtcgtgaact
     7801 cgtgcgccac cccctcgatc ttgacggagc tgacagctgc gccctcgagc gacgagagca
     7861 ggacccggcg cagcgcgttg ccgaacgtat gcccgaagcc gcggtcgagc ggttcgacct
     7921 cgaagcgcgc ccggttgccc gcgagctgct cgcgcgtgat gttgggcatc tgaaactgta
     7981 gcaactggcc tcctgtagag tcttcgccgc gggtacgttg ctgcagcgga ttacttggag
     8041 tacagctcga cgatgagctg ctcctgcacc ggggcgtcga tctcgtcgcg ctcgggagcg
     8101 cgcaggacct tgccggtgag attgtcgtgg tcgaccagga gccagggcga caccgacgac
     8161 gtgaggtctg tcgctgcgcg caccgtctcg gaggccttgc tgctggcacg gatcgccacc
     8221 acgtcgtccg ggcggacctg gtacgagggg atgtcgacct tcttgccgtt cacggtgaag
     8281 tgcgcgtgcc gcacgagctg acgggcctga cgccgcgagg cgccgaagcc gagccggtac
     8341 acgacgttgt cgaggcgcat ctcgagcagc cggagcaggt tctcaccggt gatgccgtcc
     8401 ttgcggctgg ccgtcacgta gtagtggtgg aactgcttct cgagcacctg atagtagcgg
     8461 cgcgccttct gcttctcgcg cagctggagg aggtactcgc tctgcttgat gcggccgcgg
     8521 ccgtgctcgc ccggcgggta cgcacgccgt tctacggcgc acttgtcggt gaggcagcgc
     8581 tcgcccttga gaaagagctt ctccgactca cggcgacact gcttgcactg cggtgatgta
     8641 tctttcgcca cgtggacctt cctagacccg gcgacgcttg cgcgggcggc agccgttgtg
     8701 cggcaccggc gtcacgtctt tgacactgat gatctcgagg ccggccgcct gcagcgaacg
     8761 caccgcggtc tcgcggcccg agcccggccc gcgcacgaag acctcgacct tctgcaggcc
     8821 gtgctccatg cccttcttcg cgcatgactc tgcggtcact tgcgcggcga acggggtgct
     8881 cttgcgcgat cccttgaacc cgaccgaccc ggcgctttcc cacgcgatga cgttgccctc
     8941 tttgtcggtg agcgtcacct gggtgttgtt gaacgacgtc ttgatgtgcg cctgcccgac
     9001 ggcgatgttc ttgcggacct tgcggcgctg acgacctttg gctctctggg gcttagccac
     9061 tctgctctac tcctccgctc tctgcggtca cttcttgcgc ttgccgctga tcgtccgctt
     9121 cggacccttg cgggagcgcg cgttggtacg cgtgcgctgc ccgttcaccg gcatgccgcg
     9181 acggtggcgg atgccccggt agcagccgat gtccatgagg cgcttgatgt tctgcgagcg
     9241 ctcccggcgc aggtcgccct cgacggtgac ttcacggtcg atggcgtccc gcagtttgat
     9301 gatctcttct tccgtcagat cgcgcacctt ggtgttgggg tcgatgccca cgatcgcgag
     9361 taccttgttg gaggtcgagc ggccgatgcc gtagatgtac gtgagcccga tctccacccg
     9421 cttctgcggc gggatgttga cgcctgcaat acgtgccatg cggacttacc cctgcctctg
     9481 acgatgacgc gggttcgagc agatcacttg caccgtgccg tgacggcgga tgatcttgca
     9541 cttctcgcac atctgcttga ctgatgctct taccttcatc ggtgatcctc gctggacggg
     9601 cgcggcgggc cttgcggccg tggcacaccg cctccgggcg actcctggcg agccgctact
     9661 tgaaccggta cgtgatcctg cctctgctga ggtcgtaggg cgacagttcg accttgaccc
     9721 tgtcgccggg caggatgcgg atgtagttca tgcgcatctt gcccgagatg tgggcgagca
     9781 cctcgtggtt gttgtcgagg tgaacacgga acatcgtgtt cggcagcgct tcgacaacct
     9841 cgccttcgat ctcaatggct tcttctttgt tcgccatact ccccactcgt cgacagacag
     9901 cggcctgaca tactaccaca aacggcgtgc ctcacaaacc acgccgtccg tgggggcgcg
     9961 ccggccgatg cccgcgccct ggtcttctct gctcacactc ccgcgccggc cctctccgag
    10021 cggcggcgcg tgagcacctg tggccccttc ttgcccaccg cgacggtgtg ctcgaagtgc
    10081 gccgaaagtg acccgtcggc cgtgtggatc gcccatccgt cgtcggcgac gacgacctcg
    10141 taggcgccgg cgtccaccat cggctcgatg gcgaacacca ttccttcttc cagccgcgga
    10201 ccgcggccgg ggtcaccgta gttcgggatc tgcgggtctt cgtgcatctc cttgccgaca
    10261 ccgtggccca cgagcgagcg cacgacgctg aacccggccg cctcgacgac ctcctgcacg
    10321 gcgtgtgaga tgtcgcccag gcgccggcct tcacggcact cggcgatccc cgcctcgagc
    10381 gactcgcgcg tgacgtcgag caggcgctgg gcctcgggct cgacggcgcc catcggcagg
    10441 gtgacggccg agtcggcgac gtagccctcg agggtgacgc cgacgtcgat gctgagcagg
    10501 tcgccctcgg ccgcccggta cttgccgggg atgccgtgga ccaccatgtc gttcggcgat
    10561 gcgcagatcg acgcgggaaa gccgcggtag ccgaggaacg tcggcacgcc gcggtgccgg
    10621 cggatgaact tctccgcgag gcggtcgagc tcggcggtgg tcacgccggg cgccgccgcc
    10681 tccgtgagcg cgtcgatgca gtccgcgagg atcgcgccgg cggcggcgat cttggcgatc
    10741 tctcctgccg acttacgtac gatcacgcgc ccgcgaggat ccgctggacc tgctcgtaga
    10801 cctcgtccgg ccgcttgccg ccgccgaacg cgctctgcag cacgcccttg tccgtgtagt
    10861 agccgacgag cggagccgtc tgccgctcgt acacgccgat gcgcttgcgc accgtctcct
    10921 cgttgtcgtc ggcgcgctgc accagctcgc cgccgtcgag gtcgcagacg ccgtcggccc
    10981 tgggcgggtc gaagacgacg ttgtagatct tgccgcagac cgcgcacgag cggcggccgg
    11041 cgatgcgctg aacgagctcg tcggccggga cgtcgatggc caccacgtgc gtgatggccc
    11101 tgccgccctc ggcgagcagg gcgtcgagcg cctccgcctg cgggaccgtc cgcgggaacc
    11161 cgtcgagaag gaaaccctcg gcggcgtcgg gctcggcgag ccggtcgcgg acgacgccga
    11221 tcacgacctc gtccggcacc agggcgccgc gttccatgta cgtctgcgcc tcacggccca
    11281 tcccggtggc tttggccacg gccgcgcgca gcatctcacc cgtggagatg tgcggcagcg
    11341 cgaagtcagc cacgatgcgt tccgcctggg tgcccttgcc cgcaccgggg ccgccgagaa
    11401 ggatcacgtt gcgctgcgtc atctcgtcac ctatttgagg aagccctcgt agtgccgcat
    11461 gaggagctgc gcttccatct gccgcatcgt gtcgagcgcg acgcccacga cgatgagcag
    11521 cgaggtgccg ccgaagtaga acgggacgtt gaccggcggc cgcgacagca gccacggcag
    11581 cacggccagc gccgccagga acagggcgcc ggggaacgtg agccgcgtca ggatgcggtc
    11641 gaggtactca gccgtcggcc ggccgggccg cacgccggga atgaacccgc cgtacttctt
    11701 gaggttgtcg gcctggtcga tggggttgaa cgtcaccgcc gtgtagaagt acgtgaagat
    11761 cacgatgagg atggcctcga aggtgatgaa gaggaacgtg ctcggcccga ggacccgctg
    11821 gaaccagccg ccgatgccgt cggtcccgag gaactgcgcg aaggtcggga tgatcatcat
    11881 caccgacgag gcgaagatga ccgggatcac gcccgccatg ttgaccttga gcgggatgaa
    11941 cgtgctctgg ccgcccatca tgcgccgccc gaccacgcgt ttggcgtact gcaccgggat
    12001 gcgccgctca ccggtggtga tccagatcac gccgaccacg acggccaggg cgatcaccat
    12061 gaaccccacg atcgccacca ggctcatggt gaagagctgc gccgctcccc cggggatgct
    12121 cgagacgatg ctgatgaaga tcatgatcga catgccgttg ccgatgccgc gcgcggtgat
    12181 gagctcgccg agccacatca cgaccacggc gccggccacg aggctggtca cgatcagcgc
    12241 gccgcgccac cagttgaggt ccggcagcgc gttgtactgg cggaacagga cgatgtagcc
    12301 cagcgactgc accagcgcga ggccgacggt gagccagcgg gtgtacttgg tgatctgctt
    12361 ctgaccctgc tctccctcct tggccagctc ctccagcttg gggatgacca cggtgagcag
    12421 ctgcatgatg atcgacgcgg tgatgtacgg catgatgccg agggcgaaga tcgcgacccg
    12481 gctgagcgct ccgcccgaga acaggtcgag gaagccgacg acgccggccc cgccctcttc
    12541 gaagaactgc ttcagggcct cgaggtcgat gccgggcacg ggcacgaagc tcccgaaccg
    12601 gtagatggcg accatcccgg ccgtgaagag gagcttcgtg cgaagctccg ggatcctcca
    12661 ggcgttggcg agcgactgcc agagcttgtt gttcacagcg acccccgggt gccggtcaga
    12721 ggagctcggc cttgccgccg gccgcttcga tcttctcgac ggcgctgcgg gagtacaggt
    12781 gcacgcgcac ggtgagcgcc accgtgagct cgccgccgcc gaggaccttg atgggcgtgc
    12841 gggtgttctt gacgatgccc ttggcgacga gcgcctcggg ggtcacctcg tcgccggccg
    12901 cgaacgcgtt cgcgaggcgg ccgacgttga ccggcaccgc gtacgtgcgg aacggcccga
    12961 tgggcatgct cttcttcatg tgcgggccgc gcagcttgcc gaggcgcatg tagagcggca
    13021 tctgcccgcc ctcgtagccc ggacgcacgc cgccgccgga gcgggcgttg tagcccttgt
    13081 ggcccttgcc ggccgtcttg ccctggccgg agcccttgcc gcggccgaga cgcttggtgg
    13141 ccttggtcga gcccgggttg gggcgcaggt tgtggagtcc cagttcctct gtcatgtcac
    13201 gacttcttcg ctgcagggcg gacctcgacg aggaacgcca cgtggttgac catgccgcgg
    13261 atcacggggg tgtcctcgtg caccacggtg tgccggatgc gcttgaggcc gagcgcgcgg
    13321 agcgtgccgc gatgctcggg caggcggccg atggcgctct tggtctgcgt gatctcgatg
    13381 tagtcggcca tctgcctcag gcctccttga cgtccgcagc ctcggcggcc tcaggcttgc
    13441 gctggtcgag acccaggacc tgggcgaccg agatgccgcg caggcgcgcc tggccctcgg
    13501 gcgtcttgag ggactgcagg cccgcgacgg tcgcgcgcac caggttgatg gggttggtgg
    13561 tgcccagcga cttggtgagg atgtcgcgga cgccggccag ctcgagcacg gcacggacgc
    13621 cgccggcggc gatgacgccg gtaccgggcg ccgccggctt gagcagcaca cgggcggcgc
    13681 cgtagcggcc gatcacctgg tgcgtggtgg tcgtgccgtt gtagcgcggc acggtgaaca
    13741 ggttcttctt cgcgtcttcg atcgccttct ggatcgccag cggcacctcg cgcgccttgc
    13801 cgtagccggc gcccacatgc ccgttctcgt caccgacgac gaccagggcc gtgaaggaga
    13861 accgccggcc gcccttgacg accttggcga cgcggttgat atcgacgacg cgctctttga
    13921 gctcgttgcc ttcgctgaat gatcctctag ccacggggtc ctattctcct tgtcagaaaa
    13981 ccaggccgcc ctcgcgggcg ccctcggcga gagccttgac tcggccgtga tacagatagc
    14041 ccgagcggtc gaacacgacg cgctcgatgc ccttgtcctt ggcgcgctgc gcaagcagct
    14101 cgccgacctt cttcgcgacc tcggccttct tgagaccgga gccgtcgacc tcgctggagc
    14161 gcgcggcgac gatcgtcgcc gcggcggcgt cgtcgacgag ctgcgcgtag atcgccttgt
    14221 tcgagcggaa caccgacacg cgcggccgct cggtggtgcc gctgatgcgg ccgcggatgc
    14281 gacgcttgcg cctcgcccgg gcctccctga tgctggttgc gctcatcttc tggtggttcc
    14341 tcttctctca ggcagcgcta ggcgcgcttg ccgaccttgc gctggacgta ctcgccggcg
    14401 taccggatgc ccttgccctt gtacggctcg ggcggccgga tggagcgtac cttggcggcc
    14461 acctcaccga cgacctgctt gtcgatgcct ctgacgacga ccacggtctg ctgcggcgtc
    14521 tcgaaggtga cgtgctccgg cggctcgatg accaccgggt ggctgtaccc gaccagcagc
    14581 tcgagagcgt cgcccttgat actggcgcgg tagccgacgc cgacgatctc gagacgcttc
    14641 tcgaacccgt ccgtgacgcc ggtcaccatg ttggcgatga gcgtccgggt gagcccgtgc
    14701 agcgagcggt gctggccgct gtccgtgggc cgcttgacct ggatgacgcc gtcgccgacc
    14761 tccaccatca tctcgcgggg gacccgctgg gtgagctcac ccttgggacc cttcacggtc
    14821 acgacggcgt tgtcgtactt gaactccacg ctgccgggca gcgggattgg ggcttttccg
    14881 atcctcgaca tgtcccgtcc ttaccagatg aagcagatga cctcgccgcc gaggccgagc
    14941 ttccgcgctt cagcgctggt cacgaggcct cgcgacgtgg agatgatcgc ggtaccgaga
    15001 ccgccgagca ccttgggcag ggtctcctgc ttggcgtaca cacgacgccc gggcttggag
    15061 acccgcttga tgccggagat gacgcgctcc cggttcttgc cgtacttgag ctcgaccacg
    15121 atgacctggc cgtgctcgcc ctgcttcgtg tcgtagccgg cgatgtagcc ctgctccgtc
    15181 atgaggcggg cgatctccgc cttcatcctg gaggccggga tctcgaccgt gctgtggcgc
    15241 gcggtgctgc cgttacggat gcgcgtgagc atgtcggcga tggggtccgt cattgacatg
    15301 gtcctcgcct cctcaccagc tcgacttggt cagaccgggg attacgccct ggtgcgccac
    15361 ttcgcgcaga cagatgcggc agagcccgaa cttgcggtag taggcgcgcg gacgcccaca
    15421 gcgctggcag cggttgtact gccgcgtctg gtagcgctgc ttgcgtccgg ctttcaccac
    15481 gagggatgtc ttggccatgc ttcagatgct cctagttgga acggaacggc atgcccagct
    15541 tggccagcag ggcgcgccct tcctcgtccg tcttggcggt cgtcgtgatg acgacgttga
    15601 gaccgcggaa cgtctccacc gagtcgtagt cgatctcggg aaagatggtc tgctcgcgca
    15661 cgcccatggc gaagttgccg cgcccgtcga actggccggg gtcgatgccg cggaagtcgc
    15721 ggatacgcgg gagggcgatg gacacgagcc ggtcgatcat ctcgtacatg cgctcgccgc
    15781 gaagcgtgac gcggcagccg atggacatgc cctcgcgcag cttgaagccg gcgatggact
    15841 tgcgcgcctt ggtgatcgcc ggccgctggc cggtgatcag cgccagctct tcgacggcgt
    15901 cgtcgagcag cttcgcgttc gtcttcgcct cgccgacgcc catgttgagc gtgaccttgg
    15961 tgacgcgcgg gacttccatc acgttgcggt agccgaactc gtccttgagc tcgggcacga
    16021 tctccttgaa atacctgtcc ttgagcctgg ccatctggtt acgccttgtc gatgtctttg
    16081 ccgcaactgg cgcgacggca cgtgcggacc ttgccgccgc cctcggtcag ccggtacccg
    16141 acacgggtgg gctggccgca ggaagggcag acgagcatca cgttggatgc gtcgaccggg
    16201 gcttcgcgct cgatcacgcc gccctgcggg ttggtcttgg ggttcggctt ggtgtgcttc
    16261 ttcaccaggt tgagccgctc gagcaggacg cggcgcttct cggtgtcgac gcggctcacc
    16321 ttggcggtct tgcccgcctc cttgccgctg atgacgcgca cctggtcgcc cttcttgatc
    16381 ttcatgttgg ccatcacagc acctcgggag cgagcgacac gatcttcatg aagttcttct
    16441 cgcgcagctc gcgggcgacg ggaccgaaga tgcgggtccc tcgcgggttg ttctgattgt
    16501 cgatgagcac ggcggcgttc tcgtcgaacg cgatgtacgt gccgtccttg cgaccgtatt
    16561 ccttcttcgt acgcacgatg accgcgcgca cgacgtcgcc cttcttcacg ctgccgccgg
    16621 gcgtggcgac cttgacggta ccgacgatga tgtcgccgac cgttccgtag cgccgcttgg
    16681 agccgccgcg gatccggata cagaggatct cgcgcgcgcc cgtgttgtcg gcgactcgta
    16741 gtctggtctc aacctggatc atgtgcttcc tctgccggcg ttcttacttg gctttctcga
    16801 ggacctcgag gaggcgccac cgcttggtcg cgctcagcgg gcgcgtctcg acgacccgca
    16861 cgaggtcgcc gacaccggcc gcgttctgct cgtcgtgggc gtgcagcttg ctggagcggc
    16921 gcacgacctt gccgtaccgc tcatgcttct gcgccacgtc gatgcgcacc acgatggtct
    16981 tgtccatggc gtcgctcacg acgatgccac gacgctcctt gcggcccggc accttctcgg
    17041 gcgccgcgcg ctcggccttg gccttgctgc gcgcggccga gaccttcttg gccggcttcg
    17101 cctcgggggc cacggccttc ttggcggtcg tcttcttggg cgcggccttc ttggccgccg
    17161 ccttcttcgc cgccggcttg cgggccgcgg actgcttggc agcgggcttg ccgctgtctg
    17221 tcttcttcgt cgtcgccatc aggcttcacc accggcagtg cgctcgtgct cccgctcggc
    17281 ctgcatggtg agcaggcggg cgatctcgcg gcgcgtcgca ttcagtttca tcgggttgtc
    17341 gagctgccca gtggcgtgct ggaagcgcag gttgaagagc tccttgcgcg tgtccttgag
    17401 gcgcacgatg agctcgtcgt cggagagctc gcggagcttg ctagccttca aagagactgc
    17461 cctccctcgt cacgaacttg cacttgatgg gcagcttgtg cgccgccagg cgcatggcct
    17521 cgcgggccac aggctcactg acgccggcca gttcgaacat gaccttgccc ggcttgacca
    17581 cggccaccca cgactcgggc gagcccttgc cgccgcccat gcgcgtctcg gcggccttcc
    17641 gcgtgaccgg cttgtgcggg aacacgttga tccacacctt gccgccacgc ttgatgtagc
    17701 gcgtcatggc gatacgggcg gcctcgatct ggcggttggt gatccagccg cgctcgatcg
    17761 ccttcaggcc gtactggccg aactcgacgg tcgtgccgcc cttggcctcg ccggccatgc
    17821 ggccgcgctg ttgtttcctg tgcttgactc tcttcggcag gagcatcgtc tacgtcctct
    17881 cccgtcagct ctgctcggtc ttgggcgcgt ctggcttggt ctcaggcttg ggcgcgtcgg
    17941 cgggcttggc cgagcggttc ggagcctgac gacccatggg cgccttgggg cccacggagg
    18001 gccgctggcc gccgggacca cggttctgac caccgcgacc cgggccgccg gggccacgcc
    18061 ccggaccacc gggaccgcga ccggggccgc cggggccacg gccgccgggg gcgccgtcgc
    18121 ggcgggggcg gcggtcgcga ccgccgcggt cgtcacggcg ttgctcgtcg cgctccatgc
    18181 gcgccttggc ctcgtccatc gggtagccct cgggcaggat ctcgcccttg ttgatccaca
    18241 ccttgacgcc gatgcggccg aaggtggtgc gggcctcgcg gaagccgtag tcgatgtccg
    18301 cgcgcagcgt gtgcagcggg acgcggcctt cgctgtagtg ctcgttgcgg gccatctcgg
    18361 cgccgcccag acggccgccg cagcgcacgc gcatgccggc ggcgcccgaa cgcatggcgc
    18421 tggtcagggc acgcttcatg gcgcggcgga agctcacgcg gttctcgagc tgctcggcga
    18481 tggactgggc caccagcacg gcgtcgagct ccggacgctt gacctcgatg atgttgacct
    18541 ggacgttctt ccgggtcatc gcgtggatct ctttgcgcag cgcgtcgacc tcggacccgc
    18601 tcttgccgat cacgatgccg gggcgcgcgg tgtggatgtc gatggtgatg ttgttctggt
    18661 ccttgcggat gatgatgcgc gacagaccgg cgtgcgagag cttgcgcagg atgtgcaggc
    18721 ggatgcgctc gtcctcggcc aggaactgct tgaactcctt ctccgtgtac cagtgcgact
    18781 tccagtcgtg gatgacgccg acgcggaagc cgccgggatg cactttctgg cccattagga
    18841 acggccctcc ctttgctcga ccgcgacggt gatgtggctg ctgcgcttgc ggatgcggtt
    18901 cacgcggccc atggcccgga actgccaccg cttgagggta ggaccctcgt cgacgcgcgc
    18961 ttccttgaca tacaggtcgt cggcggagag ggcgttgttg tgctcggcgt tggccacggc
    19021 ggagtcgagg accgcggcga cgagacgagc cgagttacgg ttggcgaaga acagcacgct
    19081 gcgcgcctca gccacggact tgccgcggat gaggtcggcc gcctcgcgcg ccttgcgcgg
    19141 tgcgacgcga acgtatttcg cctgtgctct gactgccatc gatgattacc ttctcgccga
    19201 gcggtcaccc ttgatgtgac cgcggaacgt acgggtgagt gcgaactcgc ccagcttgtg
    19261 accgaccatg gactcgctga cgaagatggg cacgtgcttg cgcccgtcgt ggacagcgat
    19321 ggtgtgaccg accatgtcgg ggaagaccgt ggaggcccgc gaccaggtct tgatcatctt
    19381 cttctggccg gcggcgttca tctgctcgat gcgcttcatg aggcgctcgt cgacgaacgg
    19441 acccttcttt gctgacctgc tcaaaaggtg tgcctcctgc cttacccgcg cttgccgcgc
    19501 ttgcggccgc ggatgatcaa cttgtccgac gccttgtgct tggcgcgcgt gcgatatccc
    19561 agcgtgggca cgccccacgg cgtcaccgga tgacgtccgg ccgtcgtctt gccctcgccg
    19621 ccaccgtgcg ggtggtcgac cgggttcatg gccgtgccgc gcacggtcgg gcgcacgccg
    19681 cgccagcggg tgcggccggc cttgccgccg gagaggttcg agtggtccga gttgccgagc
    19741 tctcccaccg tggcgcggca ggtgagacgg atgcgccgca actcgccgga ggggagacgg
    19801 acgacgccgt ggcccccctc tttggcctgg agctgcgcgc tcgtgccggc gctgcggacc
    19861 agctggccgc cgcgccccgg ctgaagctcg atgttgtgga tcgtcgtgcc cgacgggatg
    19921 ttctcgagcg gaagcgcgtt gccggccttg atgtcggcgc cgggaccgga ctcgacggtg
    19981 tcgccgacca cgagcgcctt cggcgccagg atgtagcgct tctcaccgtc cgcgtagtgc
    20041 agcagggcga tgcgcgccga gcggttcggg tcgtactcga tgtgcgcgac cttggccggc
    20101 acgccgtcct tgtcccgctt gaagtcgatg atgcggtagc gccgcttgtg gccgccgccc
    20161 ttgtggcgcg tggtgatgcg cccgtagctg ttgcggccgc cgttcttctt gagcggcgcg
    20221 accagcgagc gctcgggctc ggcgcgcgtg atgtcctcga acgtgtcgac cgtcatgaag
    20281 cgacggccgg gcgaggtcgg cttgaatcgc ttgatgccca ttacctgccc tcgaagaatt
    20341 cgatcttgtc gccgggcgcg agctgcacga cggccttctt ccacgccgag gtgcggccct
    20401 tgctcagacc gcggcgcttc ggcttgggac ggacgttcgc ggtgctgacg ccgacgacgg
    20461 tcacattgaa cagctgctgg atcgcctggg cgatcatggt cttgtgcgcg tcggggtgca
    20521 cctcgaaggt gtaccggctg tggtcgatct gcgcgtagct cttctcgctg atgatcggcc
    20581 ggatgatgat cttctctgct tcaaccatgg gtcacgcctc gttcagggcg gcgaacgcct
    20641 tctcggtgag caccagcgag cgggcgcgca gcacctgctc ggtggacagg gcggccacct
    20701 ccgacgcctc ggcgtacggg aggttgcgga ccgacctcgt cacgaccggt tcgtcgtgcg
    20761 tgacgaccag cacgggcgcg gcgcagtcga ggttcacgag gaactcctcg agcgtgcgcg
    20821 tgctcggctc ggacatctcg aggccggcgg cgacgaagac acggccgttg gcggcccggt
    20881 cgctcagcgc catcgcgagc gccttgcggg ccaccttctt gttgaccttg atgtcgaagc
    20941 tgcgcggcct ggggccgaac acgacgcccc cgccggtgcg gatcggcgac ttgacactgc
    21001 cggcgcgggc gcggcccgtg cccttctggc ggtacagctt ggcggtggag ccggtgacct
    21061 cgccgcgcgt cttggtggag gcagtgcccg agcggcgcgc agcctgctcg gcgcgcacca
    21121 ccaggtgcag cgtgttcacg tcggcggcga cggcgaagcg gacgtcggtc agcttgcgcg
    21181 tctcgacctc cttgccggcg gcgtcgacga aggtcgctac gccggcggcg cccttgcgcg
    21241 cctcggccgc ggccttggcg gccggagccg tcttcggcgg gcgctccgcc ttgggcgcgg
    21301 gcttctccgc ggccttggcg gccggcttct tcgcggccgg cttcttcgcg gcggccttct
    21361 tggcagccgg cttggcggcc ttctcgtcgg cggccttctc gtcggcggcc tcggccttgg
    21421 cggccggctt cttcgccgcc ggcttcctgg ccggagcctt ctcgtccgcc gtcgcgtcgt
    21481 ccgccttgtc ggcggccggc ttcttggccg cgggcttcct ggcggcaggc ttcttcttgg
    21541 gggcctcggc ctcggtcttg ccggccgcct cagcctcggt cttctcgggc tcggcggcgg
    21601 cctcgacgtc gggggtcttc ttgatctcgt cggcgctcac tgctacctac ccttgatgac
    21661 gacgacggag ttcttggcgc ccggcacaga gcccttgatg agcagcaggt tgttctcgac
    21721 gtcgcgacgg acgatcttga ggccctgctg ggtgacgcgc tgcgccccca tgtgcccgct
    21781 catgcgcatg cccttgaaca cccggctggg atacgcgctg gcgccgatgg aacccggctg
    21841 tctgacgttg tgcgaaccgt gggtctccgg gccgcggccg aagttgtgcc gcttgatggt
    21901 gccctggaac cccttgccct tgctcacgcc ggtcgcggtg acggcctggc cgtcctcgaa
    21961 cgcctcgacg gtgacggtgt cgccgacctt gaggtcgccc acaccgtcga actccacgag
    22021 gtggcgcatg ccgtgcttga ggccggcctt cttgaggtga ccgagctcgg gcttggtgat
    22081 gtgcttgtcc ttgcactcct cgaacgcgag ctgcacggcc tcatagccgt cgttgtcgag
    22141 ggtgcgtacc tgagtgacgg gacaggggcc agcctgcacg acggtgacgg cgatcacgtt
    22201 gccgtcctcg tcgaagagct gggtcatgcc catcttcttg ccgatgatgc cagtcatcgc
    22261 tctacagctt gatctcgatg tggacaccgg ccggcagatc gagacgcatc agctggtcga
    22321 ccgtcttggg ggtcggctga tggatgtcga tgagcctctt gtgggtacgg atctcgaagt
    22381 gctcgcgtga gtccttgtcc ttgaacgggc tcttgatcac gcaatacacg ttcttctccg
    22441 tggggagcgg cacggggccg gagatggtcg cgccactgcg ctgcgcggtt tccacgatgg
    22501 actgcgcgct tcgctcgatc agctcgtgat cgtaagactt caaccttatg cggatcttct
    22561 gctgtgccac tctcgccttc tctgtgtcct gttcacgcgg ccgggcgtgt cgcgcacgcg
    22621 agcgtgcggg atgcacgccc ggcccgatct ggccttcgtc ttacgcgatg atcttggtga
    22681 tggccccggc gcccacggtg cggccgccct cgcggatggc gaagcggagg ccctcttcca
    22741 tcccgatcgg ggtgatgagc ttgacttcca tcaccgtgtt gtcgcccggc atgaccatct
    22801 cgacgccctc ttcgagggtg atgacgccgg tcacgtcggt ggtgcggaag tagaactgcg
    22861 ggcgataccc gttgaagaac ggcgtatggc ggccgccttc gtccttggag agcacgtaga
    22921 cctgcgtgag gaagtgcgtg tgcccggtga tgctgcccgg cttggagcag accatgccgc
    22981 gctcgacctc ttcgcgcttg acaccgcgaa ggagcaggcc gatgttgtcg ccggcgcgac
    23041 cttcgtccag cagcttgcgg aacatctcga cgccggtcac ggtggtcttc atcggcttgt
    23101 cgcgcatgcc gacgatctcg acctcctcgc cgaccttgac gatgccgcgc tccacacggc
    23161 cggtggcgac ggtgccgcgg ccggtgatgg tgaacacgtc ctcgacgggc atgaggaacg
    23221 gcttgtcgat atcacgctcg ggctcgggca ggtactcgtc gagcgcgttg gtgagctcga
    23281 tgatgctgcc gcaccactcg cagtcgtcct tgccgcagcc acactcgagc gccttcagag
    23341 ccgagccctt gatgacgggc aggtcgtcgc ccgggaactc gtactcgctg agcaactcgc
    23401 ggacctccat ctcgaccagc tcgatgagct cggggtcgtc gacgaggtcc gtcttgttca
    23461 tgtacacgac gatgttcgga acgttcacct ggtgcgccag gaggatgtgc tcgcgggtct
    23521 gcggcatcgg gccgtcgtcg gcgccgacca ccaggatcgc ggcgtccatc tgtgcggcgc
    23581 cggtgatcat gttcttgatg tagtcggcat ggcccgggca gtcgacgtgc gcgtagtgac
    23641 gcttctcggt ctcgtactcc acgtgcgcgg tggcgatggt gatgccgcgc tcgcgctctt
    23701 ccggcgcctt gtcgatcatg tcgaacgcgg tgaactcggt gttgccaccg cggttgtgca
    23761 gcacgagcgt gatcgcagcg gtgagggtgg tcttgccatg gtcgacgtga ccgatggtgc
    23821 ccacgttcat gtgcggcttg ctgcggtcaa acttctgctt ggccatctct gctactcctt
    23881 ttcccgaaac ccggaggttg aggtcacaca aaaagcggcg cggcgacctg ccgcaccaaa
    23941 ggtcggcagt atacacgacg ccggcctgct tgggcaaaca gtgtgggcgt tcatcccggt
    24001 catccgcgca ccttggcgac gatctcggtg gcgatggacg cgggcacctc ggcgtagtgc
    24061 ttgaactgca tcgtgtaggt ggcacgcccc tgggtggacg agcgcaggtc ggtggcgtac
    24121 ccgaacatgg tggagagcgg caccgtggcg ttgatcacct gcgcgttgcc gcgcggctcc
    24181 atgccgtcga tgtggccgcg gcgcgagttg atgtcgccca tcacgtcgcc catgaaatcg
    24241 gccggggtga cgacctccac ggccatcatc ggctcgagca ggaccggctt ggctttctgc
    24301 gcggcgttct tgaacgccag cgagccggcg atcttgaacg ccatgtccga cgagtcgacc
    24361 tcgtggtagc tgccgtcgac gagcgtcacc ttgatgtcga ccatcggata gccggcgagc
    24421 acaccggtgc tcatggcctc ctggatgccg tcgtcgaccg acgggatgta gtcccggggg
    24481 acgactccgc ccatgatctt gctctcgaac tcgtaaccgc cgcccggctc gttgggcgcc
    24541 atgtcgatgc gcacgtggcc gtactggccg cggccgccgc tctggcgcac gaaccggcct
    24601 tcgaccttct cgaccttgtt gcggatggtc tcgcggtacg cgacctgcgg ccggccgacg
    24661 ttggcgtcga ccccgaactc gcgcaccagg cggtcgacga tgatctcgag gtggagctcg
    24721 cccatgccgg cgatgatcgt ctggccggtc tcctcgtcgg tgtggacgcg gaaggtcggg
    24781 tcctcctcgg cgaggcgctg cagcgagttg ccgagcttct cctcgtcggc cttggtcttg
    24841 ggctcgatgg ccacctggat gaccggctcg gggaactcca tggactcgag gatgacgggc
    24901 ttggacgggt cgcacagcgt gtcgccggtc cccgtgacct tgaggccgac ggcggcggcg
    24961 agctcgcccg cgtagatcac gtcccggtcc tcgcggtggt tggcgtgcat ctgcaggatg
    25021 cggctgatgc gctcgcgctt gtcacggctc gagttgagga cgtacgagcc ggccttgagc
    25081 gtgcccgagt agacgcggaa gtaggtgagc ttgccgacgt aggggtcgac ggcgaccttg
    25141 aaggccaggg ccgagaacgg cgcgtcgtcc gccgtctgcc gcgtgaccac ctcgccgtta
    25201 ggcttggtcc cgtcgacggc cggcacgtcg agcggcgacg gcaggaagtc gacgatcgcg
    25261 tccatgagcg gctgcacgcc cttgttcttg aaggcggagc cgcacatcac cggggtgatg
    25321 ctgatgtcga gggtggcctg ccggatgacc tcgcgcaggt gcccgggggt gatctcctcg
    25381 ccctcgagat acgcctccat caggtggtcg tcatggtcgg cgatggactc gacgagcttg
    25441 agacggtact cgtccgccag ttcgcggtga tcgtccggga tgtcggtgac ctcgaagtcg
    25501 gtgcccagtt cgtcgaggta gatggtggcg ttcatctcga tgaggtcgat gatgccgcgg
    25561 aactcggcct cgcggccgat ggggatctgc agcgccacgg ggttggcccc gaggcggtcg
    25621 accatcatct cgaggccgcg atagaagtcc gcgccgatgc ggtccatctt gttgacgaac
    25681 gcgatgcgcg ggatgccgta cttgtcggcc tggcgccaca cggtctcgga ctggggctcc
    25741 acgcccccga cggagcagaa cagggcgatg gcgccgtcga gcacgcgcag gctgcgctcg
    25801 acttccatgg tgaagtccac gtgcccgggc gtgtcgatga tgttgatctg cgtgtcgcgc
    25861 caggagacgg tcgtcgcggc cgacgtgatc gtgatgccgc gctcctgctc ctgcgccatc
    25921 cagtccatga ccgcggcgcc ctcgtggacc tcgccgatct tgtgcgtctt ccccgtgtag
    25981 tagaggatgc gctcggtcgt cgtggtcttg ccggcgtcga tgtgcgccat gatcccgatg
    26041 ttgcgggtgt tgtccagcga atactgtcgc atgctgctcg tcgcgttcct tccatgcgaa
    26101 agcccctcac cgacgaaggt cagtgagggg ccctcggcgg attaccagcg atagtgcgcg
    26161 aatgccttgt tggcctgcgc catcctgaag atgtcgtcct tcttcttgaa cgcgccgccc
    26221 tgcccggcga gagcgtcgag catctcggcg gccagccgct cggccatgac cttctcgcga
    26281 cggtcgcggg cgaagctcac gagccagcgg atggcgagcg tgcgggcgcg gcggccgggc
    26341 acctcgacgg gcacctggta ggtggcgccg ccgacgcgcc ggctgcggac ctcgaggtgc
    26401 ggcgtgagcg cctggatggc gtcctgcagc acctcgaccg ggtcgcggcc ggtcttctcg
    26461 cgcacgatct cgagcgcgcc gtagacgatc tgctcggccg tgctcttctt gccgtcgagc
    26521 aggaccttgt tgatgacctg cgtgacgagc ttgctgttgt agacggggtc cgggagtact
    26581 tcccggcgcg tgatggtgcc tctgcgcggc atgtctacct caccccttcg ccttggatcc
    26641 gtagcgcgag cgcgccttct tgcggtcggc tacgccggag gcgtcgagag acccgcggat
    26701 gaccttgtag cgcacgcccg gcaggtcctt gacgcggccg ccgcgcacga gcaccaccga
    26761 gtgctcctgc aggttgtggc cgatgccggg gatgtacgtg gtgacctcca tgccgttgac
    26821 cagccgcaca cgggcgacct tgcgcagggc ggagttcggc ttcttgggcg tcaccgtgta
    26881 gacgcgcgtg catacaccgc gcttctgggg tgaacccttg agcgccgggg tcttcgaacg
    26941 cttggacttg ctctcacgcc ccttgcggac gagctggctg atggttgtca tctgacctct
    27001 ctggtctttc tcatgtgagc cgctgtggac aacggccatg tagtctagcg aaacggggct
    27061 gcggccgcaa catgcgcctt tcggcggccg cagccccgcg tgtggtcggt ccgctccccg
    27121 gcctactcga tctcgccgtc gaggccgtca ccgtcgccga ggctccaggc gggatcgccg
    27181 gtcatgagac ggtcggcctc ggccagcgct tccgcccggc cggcggcgtg gatctcgacg
    27241 ccgcggtagc ggcgcagacc ggtcccggcc gggatgagct tgccgatgat gatgttctcc
    27301 ttgaggccgg cgaggttgtc gaccttgccc tcgatggccg cgtcggtgag caccttggtg
    27361 gtctcctgga aggaggcggc gctcaggaag ctctcggtgg cgagcgaggc cttggtgatg
    27421 ccgaggatga tctcctcggc ctcggcggcc tcggcgccct cggcggacat gcgctcgttc
    27481 tccgccacga actggtagcg gtcgacgagc tggccgggca ggaaggtggt gtccccggcg
    27541 gtcacgatgc gcaccttgcg catcatgcgc cgcacgatga gctcgatgtg cttgtcgttg
    27601 atctccacgc cctgcgagcg gtagacctcc tgcacctcgt tgacgaggta cttctcggtg
    27661 gccgtggcgc cggcgtgctt gagcaggtcg gccggataga gcgagccggg cgtgatctgc
    27721 gtgccctcct cgacccagtc gtcgtcctcc accatcacgt gcgtgcggcg ggcggcgatc
    27781 gtgtactcct tggcggcgcc ctcgtccggc gtgatcgtga ccttgcagcc gcgctccgtg
    27841 tcctcgatgg acacgcgccc ggtcacctcg gcgatgcgcg cgacgccctt cggcttgcgt
    27901 gcctcgaaga gctccacgac gcgcgggagg ccgtgcgtga tgtcggcgcc ggcgatgccg
    27961 ccggtgtgga acgtgcgcat ggtgagctgc gtgcccggct cgccgatgga ctgcgccgcg
    28021 acgtggccga cggcgtcgcc gatctcggcg ggcgcgttgt tggcgagcgc gtacccgtag
    28081 cacttctggc acacgccgat ctcggcgtcg cacttgagca cgctgcggac cggcaccatc
    28141 gggtcggtct gcgagctgcg cgcgaggatg tcgaggatct ggtcgcgggt cgcgacgtcg
    28201 atgtgcgtgt cgcggtcgaa gacgacctcg cccgactctt cctcgacgat ggcgtcgccg
    28261 aggtgcctgc cgagcaggtt ctcgttgggc tcgtgcgtcc acacggccat catcgccgca
    28321 gcggccttgc ccttcttcac cttgaccttg gcgttgggca gcatggagta gacctcacgc
    28381 agcaggccga tctgggccgg gccgatcttc tcgcccttct tgagcacgat gcggtcgctg
    28441 cgcggccgcc tgacgtcctc ggcgagcacg gcgccgtcga gtcccgggtt ctcctcgcga
    28501 cgccgcgacc acggcgacat gggcacccat tccttggtgc cgcagtcgat gtcgctgacg
    28561 gtgacgtcct ggcacacgtc gaccagacgc cgggtgaggt acccggagtc ggcggtgcgc
    28621 agggcggtgt cggcgagacc cttgcgggcg ccgtgggtgg agatgaagaa ctcgagcacg
    28681 gagaggcctt cgatgaagct cgacttcacc ggctgctcga cgatctcgcc cttcggattg
    28741 gccatctgac cgcgccagcc ggcgagctgg cggatctgct tgatgtcgcc gcgggcgccg
    28801 gaggtggcca tcatgtagat cgggttgagc gggttgaagt tggccctggt ggcggcggtg
    28861 atctgcacgt cggcttcacg ccagatctcc tgcaccttgc cgcgccgctc ctcctccgag
    28921 aggaagccgt tgttgtactc gtcccggagc tggtcgacca tcgcctcgtg ggtgccgagg
    28981 atctcggcct tctccttggc ggggacgacg acgtcgttct tggacacggt gacggcggcg
    29041 cgggtcgcga agtggaagcc gacctccttg aacacgtcga ggatgcgggc caccttgttg
    29101 gcgccgtaca gggccaccag cttgttgatg aaggcggcca ggcccttctt ggtcatcgtg
    29161 tagttgatga aggtgtagtc gccggcgtac tcctcgccga gcacctcgcg gaggccctct
    29221 tcgacctcca tgttgaagag cgcgcggccg acggtggtga cgacgcgctc gcccgtcgtc
    29281 agacggagca ggatggggct gcgcgtgaac tcggcgatgc cggccgagcg cttggtgggc
    29341 acctcacgct ggtcgaggat gcgctgctcc cacgcgcgct ggacctcctc gtagcccgag
    29401 taggcgtgca gctgctcgcc gaagtggtcc ggctgagcct cgctcaggtc gtcctcggcg
    29461 tggtaggtga ggtagtacac gccgatgacc atgtcctggc tgggcacggc gatgggcttg
    29521 ccgtgcgccg gggagaggat gttgttggtg ctgagcatga ggacgcgggc ctcggcctgc
    29581 gcctcccagc tcaggggcag gtggacggcc atctggtcgc cgtcgaagtc ggcgttgaac
    29641 gccgcgcaga cgagcgggtg gatctggatg gccttgccct cgacgagcag cggctcgaac
    29701 gcctggatgc cgagccggtg cagcgtcggc gcgcggttca gcatcaccgg gtgctcggtg
    29761 atgacctcct cgaggatgtc ccagacctcg ggcaccatcg actcgaccat cttcttggcc
    29821 gccttgatgt tctggaccat cttgcggccc acgaggcggc tcatgatgaa cggcttgaag
    29881 agctcgagcg ccatcatctt ggg
//
LOCUS       DASUMX010000318         5020 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1995797, whole genome shotgun sequence.
ACCESSION   DASUMX010000318 DASUMX010000000
VERSION     DASUMX010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 5020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5020
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1995797"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            204..767
                     /locus_tag="VFZ86_15125"
     CDS             204..767
                     /locus_tag="VFZ86_15125"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5643648.1"
                     /translation="MDAVIRPLRETDRPAVARLLDDAVGAGFWRVADGADATSFVAVS
                     GTGEGAGVVLACLEPAGDPDAQTALNGSSAAGAAAGALVLHVRQLAVAPAARRSGLAS
                     RLLARTEAEALARGATAAYAFGWLPAGRPEPDAVPFYSAAGYEARPDIPGFYAESSLA
                     TGARCPYCGEPPCRCAARPFVKALGPG"
     gene            complement(795..1736)
                     /locus_tag="VFZ86_15130"
     CDS             complement(795..1736)
                     /locus_tag="VFZ86_15130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012633010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEX5643649.1"
                     /translation="MTEQARTVERVITPQTVMEGAGVRLKRSIATPTVDYVDPFLLFD
                     HFGSDRPADYLAGFPWHPHRGIETVTYMLAGEVDHRDSIGNAGRIGAGDVQWMTAGGG
                     IMHEEMPQPGEDERMGGFQLWVNLPASLKMSHPRYQDLSAAQVPEVRRDDGTVIRVVA
                     GEVDGVAGAVAEIYAEPEYLDVALPAGAKFAQPVPRGHAALAYAFEGEGRFGVGCDLG
                     YRGDLDGVQLTAPQMAVFGDGDEVRVEADDAPVRFLLISGKPLGEPIARYGPFVMNTR
                     EEISLALRDLQNDTFVWRDDPEWAAKRRTWSPETWER"
     gene            complement(1840..>5020)
                     /locus_tag="VFZ86_15135"
     CDS             complement(1840..>5020)
                     /locus_tag="VFZ86_15135"
                     /inference="COORDINATES: protein motif:HMM:NF021504.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HEX5643650.1"
                     /translation="GGRSLSIVEIGGQDAKFINVENGRVVDADMNRVCSAGTGSFLEE
                     QAVAHGVQDIAEFGRLAAGSDGPPDLGQTCTVFVADVAAEALGDGYTRADIFAGLQYS
                     VVRNYRSRVMGQRRLLGRVFFQGKPATDPALARTLAAVLEREVIVPADPGAMGAVGIA
                     LLTARTLAAGEEPATVDLGRVLDARVAGRREFQCGDRDCRNSCHLELAEIVVGGAVEK
                     IVSGGQCPKYDAVSAAGEKLPKAAPNPYRERDDLLEALLDEEKGRAGDAGDVAAGGAT
                     GDPLVGLPYAHYLIDTLPFFHTLLSRLGYRVDVLRPGHDTLADGDRTCAAPGACAPVK
                     LLHGLAGADVDVFFAPIFVHLPLPNAGAGTYTCPMAQGAPDMVGRALQAGGARSRVLR
                     PVLFEKEGDGFEAPRVRRSVSRAMRALAEAAGRQALTPRAFEEAYVAALDAQRRFQDG
                     LGAIGERGLAWAREHGYPVVLIVGETHVIHDAVLDAGVHELVAANGALPLPVDCYPTP
                     EDAPGLRRVHWASAGQALRATAAGAIAGDVYPLLLGAYGCGPNSMIEHLFTDLTEEWP
                     HAVLESDGHGGKAGYVTRVQAFLHSVRRWRETAADGAGAVGEPADGPDSGAGGAAAAG
                     RVAAHDAAGAGMQARLARLDRRLPHSLDAGYDRFIFGHVGGGLGRHLAAAMRGAGLDA
                     SYVGEPDAAALRAAGAACSGKECLPYQLIWGSFARFLAEEEPRLNGGKALFLSAGNGF
                     QACRANLFPLTEQISLERLGLGDRVEVADFSLVTGNVRMMPIVWIALVAVDLLNMLRF
                     HSLADEAEPGASDALFRDSSDRLEALLERPRPDTGLAGDVAGAFRFCAEVEGLLGEVS
                     RAIAALPRDAARTAGLRDVYLCGDLYLRVDEWGNDGLQRKLAGHGLRVIFEPYGAFFE
                     LLQLRQIQDGLPLTKVAEKEATLATMRYVIGRLVGAVRAEHPWVFWNEVRDVDRESRR
                     LVAPYPFGETIPTVGGALLSWRTQPIDGVVSVAPRGCGPALIAEAQLRRETRLPALFV
                     YNDGDPIDEARLAGFAWRLRSRPARRAAD"
BASE COUNT          643 a         1916 c         1814 g          647 t
ORIGIN      
        1 catcgactac gtgctcgtcg accgtcgctg ggacgtcctg gccgcgcgca tcgaccacgc
       61 gcggccgggc gggcgtctgc cctcggacca ctggccggtg gtggcgacga tcgcgctgcg
      121 gccgtccggc gcgacctgac cggcgcgcac tgcgcggacg ccagcggcga ccccgtgacg
      181 ccggtggcgg tacactcgcc cccatggacg ccgtgatccg ccccctgcgc gagaccgatc
      241 gccctgccgt cgcccgcctg ctcgacgacg ccgtcggcgc cggcttctgg cgcgtcgccg
      301 acggcgcgga cgcgacgtcg ttcgtcgcgg tgtccggcac cggagagggg gccggcgtgg
      361 tcctcgcctg cctggagccg gccggcgacc ccgacgcgca gacggccctg aacggttctt
      421 ccgcggcggg cgccgcggcc ggtgcgctgg tcctgcacgt gcgccagctc gccgtcgcgc
      481 ccgcagcgcg acgcagcggc ctcgcctcgc gtctgctggc ccggacggag gccgaggcgc
      541 tcgcccgcgg cgccacagcg gcctacgcgt tcgggtggct gcctgccggc cggccggagc
      601 cggacgccgt gcccttctat tcggcggccg gctacgaagc ccggccagac atcccgggct
      661 tctacgcgga gagcagcctg gcgaccggtg cccggtgccc gtactgcggc gagccgccgt
      721 gccgctgcgc ggcgcggccg ttcgtcaagg cgctggggcc gggctgagcc cggcgggcgc
      781 cgccggcggc gccgtcaccg ctcccaggtc tccggcgacc acgtgcgccg cttcgccgcc
      841 cactccgggt cgtcgcgcca cacgaaggtg tcgttctgca ggtcgcgcag cgcgagcgag
      901 atctcctcgc gcgtgttcat cacgaacggc ccgtaccggg cgatgggctc gcccagcggt
      961 ttgccgctga tcagcaggaa ccgcaccggg gcgtcgtccg cctcgacgcg gacctcgtcg
     1021 ccgtcgccga agaccgccat ctgcggcgcc gtgagctgga cgccgtcgag gtcgccgcgg
     1081 tagccgaggt cgcagccgac gccgaaccgg ccctcgccct cgaacgcgta cgcgagggcc
     1141 gcgtgcccgc gcggcaccgg ctgcgcgaac ttcgcgccgg cgggcagcgc gacgtccagg
     1201 tactccggct ccgcgtagat ctccgccacc gcgccggcca cgccgtccac ctcgccggcg
     1261 acgacgcgga tcaccgtccc gtcgtcgcgc cgtacctcag ggacttgcgc ggcgctgagg
     1321 tcctgatagc gcggatgtga catcttgagc gacgccggca ggttcaccca gagctggaac
     1381 ccgcccatcc gctcgtcctc cccgggctgc ggcatctcct cgtgcatgat gccgccgccg
     1441 gccgtcatcc actggacgtc gccggcaccg atcctccccg cgttaccgat gctgtcgcgg
     1501 tggtcgacct ctcccgcgag catgtaggtc accgtctcga tgccgcggtg cgggtgccag
     1561 gggaaccccg ccaggtagtc ggccggccgg tccgacccga agtggtcgaa caggaggaag
     1621 gggtccacgt agtcgacggt cggggtggcg atgctgcgct tgaggcgcac gccggctcct
     1681 tccatcaccg tctgaggagt gatgacgcgc tccacggtgc gggcctgctc agtcatagct
     1741 gcgctcctcg ggaggtgtgg tcgtcggcga aaaccttccc tctcggggag gacgaaaacg
     1801 tgaggagtga gacttcgcct cgcgcctctt ccggcgcgtt cagtccgcgg cgcggcgtgc
     1861 cggccggctg cgaagcctcc aggcgaagcc cgcgagccgg gcctcgtcga tggggtcgcc
     1921 gtcgttgtag acgaagagcg ccggaagacg ggtctcgcgg cgcagctggg cctcggcgat
     1981 cagcgcgggc ccgcatccgc ggggagccac cgacaccacc ccgtcgatgg gctgcgtgcg
     2041 ccagctcagc agcgccccac cgaccgtcgg gatcgtctcg ccgaagggat acggcgcgac
     2101 cagcctccgg ctctcacggt cgacgtcgcg cacctcgttc cagaacaccc acgggtgctc
     2161 cgcacgcacc gcccccacca gccgtccgat cacgtagcgc atcgtcgcca gcgtggcctc
     2221 cttctcggcc accttcgtca acggcaggcc gtcctggatc tggcgcaatt gcaggagctc
     2281 gaagaacgcg ccgtacggct cgaagatgac gcgcaggccg tggccggcca gcttgcgctg
     2341 caggccgtcg ttcccccact cgtcgacgcg caggtagagg tcgccgcaga ggtacacgtc
     2401 gcgcaggcca gcagtcctcg ccgcgtcgcg cggcagcgcg gcgatggcgc gggagacctc
     2461 gccgagcagg ccctcgacct cggcgcagaa acggaaggcc ccggccacat cgccggcgag
     2521 gccggtgtcc ggccggggcc tctcgagcag cgcctccagc cggtccgagg agtcccggaa
     2581 cagcgcgtcg ctcgcgccgg gctcagcttc gtcggcgagg ctgtggaagc ggagcatgtt
     2641 gagcaggtcc acggccacca gcgcgatcca cacgatgggc atcatgcgga cgttcccggt
     2701 cacgaggctg aagtcggcga cctccacgcg gtcgccgagg ccgagccgct ccagcgagat
     2761 ctgctcggtg agcgggaaga ggttggcgcg acacgcctgg aagccgttgc cggcgctcag
     2821 gaagagagcc ttccccccgt tcaggcgcgg ctcctcctcg gccaggaagc gggcgaagct
     2881 gccccagatg agctgatagg gcaggcactc cttgcccgag caggccgcgc cggcggcgcg
     2941 gagagcggcc gcgtcgggct cgccgacgta ggacgcgtcg agaccggcgc cgcgcatcgc
     3001 cgccgcgagg tggcggccga ggccgccgcc gacgtggccg aagatgaacc ggtcgtagcc
     3061 ggcgtccaga ctgtgcggga gacgccggtc gaggcgcgcc aggcgcgcct gcatgccggc
     3121 gccggccgcg tcgtgcgcgg ccacgcgacc ggccgccgcg gcgccgcccg cgccgctgtc
     3181 ggggccatcc gcgggctcgc cgaccgcgcc cgcgccgtcg gcggccgtct cgcgccagcg
     3241 ccgcacgctg tggaggaacg cctggacgcg cgtcacgtag ccggccttgc cgccgtgacc
     3301 gtcgctctcg agcacggcgt gcggccactc ttcggtgagg tcggtgaaga ggtgctcgat
     3361 catcgagttc gggccgcagc cgtacgcgcc gagcagcaac gggtagacgt cgccggcgat
     3421 ggcgccggcc gccgtggcgc gcagcgcctg gccggcgctg gcccagtgca cgcgccggag
     3481 ccccggcgcg tcctccggcg tcgggtagca gtccaccggc agcggcagcg cgccgttggc
     3541 cgcgaccagc tcgtgcacgc cggcgtcgag cacggcgtcg tggatcacgt gcgtctcgcc
     3601 gacgatgagg acgaccggat agccgtgctc gcgcgcccac gcgagcccgc gctccccgat
     3661 cgcccccagg ccgtcctgga agcggcgctg cgcgtcgagc gcggccacat acgcctcctc
     3721 gaacgcccgc ggcgtcagcg cctggcggcc ggcggcctcc gcgagggcgc gcatcgcgcg
     3781 ggagacggac cgccgcaccc gcggcgcctc gaacccgtcg ccctccttct cgaacagcac
     3841 cgggcgcagc acgcgcgagc gcgcaccccc ggcctgcagc gcccggccca ccatgtcggg
     3901 cgcgccctgg gccatggggc acgtgtacgt cccggcgcct gcgttgggca gcggcaggtg
     3961 cacgaagatc ggcgcgaaga agacgtcgac gtccgccccg gccagcccat gcagcaattt
     4021 gaccggcgca caggcgcccg gggcggcgca ggtgcgatcg ccgtccgcca gcgtgtcgtg
     4081 ccccggccgg agcacgtcca cgcggtagcc gagacggctc agcagcgtgt ggaagaacgg
     4141 cagcgtgtcg atcaggtagt gcgcgtacgg gagcccgacg agggggtcgc ccgtggcgcc
     4201 gccggccgcc acgtcgccgg cgtcgcccgc cctccccttc tcctcgtcca ggagcgcctc
     4261 gagcagatcg tcgcgctcgc gatacgggtt gggcgcagcc ttggggagct tctcgcccgc
     4321 cgccgagacc gcgtcgtact tggggcactg gccgccgctc acgatcttct cgaccgcacc
     4381 gccgacgacg atctccgcaa gctccagatg acagctgttg cggcagtccc ggtcgccgca
     4441 ctggaactcc cgccgtcccg cgaccctggc gtcgagcact cgcccgaggt cgacggtcgc
     4501 cggctcctct ccggcggcaa gcgtccgcgc ggtcagcagc gcgatgccga cggcgcccat
     4561 cgcgccgggg tcggccggca cgatcacctc gcgctcgagc acggctgcga gcgtgcgcgc
     4621 gagcgccggg tccgtggccg gcttgccctg gaagaacacg cggcccagca gccggcgctg
     4681 gcccatgacc cggctgcggt agttgcgcac cacggagtac tgcagcccgg cgaagatgtc
     4741 ggcgcgggtg tagccgtcgc cgagcgcctc cgcggccacg tccgcgacga acacggtgca
     4801 ggtctgaccg aggtcgggag gcccgtcgct gcccgcggcc agccgcccga actcggcgat
     4861 gtcctgcacg ccgtgggcga ccgcctgctc ctcgaggaac gaaccggtgc cggcgctgca
     4921 gacgcggttc atgtcggcgt cgacgacgcg gccgttctcg acgttgatga acttggcgtc
     4981 ctggccgccg atctcgacga tcgagaggct gcggcccccg
//
LOCUS       DASUMX010000319        12198 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1275420, whole genome shotgun sequence.
ACCESSION   DASUMX010000319 DASUMX010000000
VERSION     DASUMX010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 12198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12198
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1275420"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..657
                     /locus_tag="VFZ86_15140"
     CDS             172..657
                     /locus_tag="VFZ86_15140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643651.1"
                     /translation="MRAETKRQRVFLTFAAGDLAQARPVTARLVAGGGLALDQALTSE
                     PFATVRGDIIRASLLTRLRHCASALCLYGARTVDDDWVWWALETAAELKLPLLGAALP
                     GEDAKDAERLLTRLGAEIVPLNGEAIVARARAASSFPAARAIDAAAIAETLHLMRHPL
                     R"
     gene            669..1493
                     /locus_tag="VFZ86_15145"
     CDS             669..1493
                     /locus_tag="VFZ86_15145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HEX5643652.1"
                     /translation="MLQPPVVITAALTGSRITREQTPAIPVTPEEIAAEGIAAWRAGA
                     AIVHVHVRDAAGRGAQDMDAFRRVVSVLRAETDVVLCLTTSGIPGRNLPTAERIAPLA
                     LRPEMASFDAGTIRMDAGMFVNEPEFLDALAREAREQGVRLELECFDLGMVETALRYH
                     AAGKIPAPLHFQFVTGTRYGMPATAQALVAAVDLLPTDATWSVIGVGRNQTPMAMLAL
                     AMGGHVRVGLEDNIHYRRGELATGNAQLVERAVRLAREIERPVATPDQARGILGLR"
     gene            complement(1504..3144)
                     /locus_tag="VFZ86_15150"
     CDS             complement(1504..3144)
                     /locus_tag="VFZ86_15150"
                     /inference="COORDINATES: protein motif:HMM:NF004718.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5643653.1"
                     /translation="MQVQLRLGQAADEPGDGRVVAGEDPGVGGGGHAASFWASVDPLY
                     PRAWVVARGAPPARVRSTPRCGDALQAAARPDRVDSVEREHVIYSWSAQGGIDPVRVV
                     GGEGCWFRDSQGRRYLDFASQVVNLNLGHGHPRLVEALRRQAEALYGLAPSFASEPRS
                     ELARLLAEVTPGDLGMTLFTTGGADANENAVKLARWVTGRQKVVARYRSYHGATAGAA
                     ALTGDPRRWPAEPAAPGVVRMFDPYPYRCSAGHSYDALGAHAFDPTAGHVDDCAIAAG
                     GPHLEEILQFEGPGSVAAVILETVTGANGVLVPPPGYLASIREVCDRHGILLILDEVM
                     TGFGRTGRWFACEHWGVTPDIMTLAKGISGGAAPLGAVVVSEKLRPWLEEHEFASGLT
                     ACGHPLACAAGVATVETLREERLVENAAVMGEILAAKLLELADDHTSVGEVRGLGLLW
                     GLELVRDRETREPLVPYAATGEAAQPMAALRRAAMDRGLHLYVHGNLLLIAPPLVVGS
                     DELDTGLAILDEVLAVADGFAGRGSVAGSDPARGSGST"
     gene            3049..4101
                     /locus_tag="VFZ86_15155"
     CDS             3049..4101
                     /locus_tag="VFZ86_15155"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEX5643654.1"
                     /translation="MSATTDPGILTGDDTAVAGFIRSLPKAELHLHIEGTLEPELLLE
                     LGRRNGIALPCSTAEECRAQYRFHDLQDFLDIYYAGVTVLVSEQDFYDLTAAYVRRVI
                     ADGARHVEVSFDPQSHVPRGVPFADVVGGITRALRDGDAGHGLSWRLIMCFLRDRPAS
                     EAMEMLELALPHRDVIAGVGIDSAEAGHPPGEFAEVYARARAAGLVAVAHAGEEGPPA
                     YISEALDLLAVRRIDHGVAAIQDAELQRRLAAEQIPLTMCPLSNLELQVTPDLSRHPL
                     KRLLDAGLVVTINSDDPAYFGGYLLDNYVAAQKALGLTRADLAQLARNSITASLLPEE
                     RKAELVAEIDRVAAGG"
     gene            complement(4145..4924)
                     /locus_tag="VFZ86_15160"
     CDS             complement(4145..4924)
                     /locus_tag="VFZ86_15160"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5643655.1"
                     /translation="MRGLTYKVALITGGSSGLGEAIVHRFAEEGVSVAVGGRDAARTQ
                     AVAAHAAELARANGHASEHAVVLGDVSVVADCDRLVAETLERFGHLDILVNSAGIWIE
                     KPTTEMSEDEYDRLMGVNLKGAYFMCRAALRHMTARRSGVIVNIASDSGIHGEPNAAA
                     YSAAKAGIVMMTRAMAIDHGPDGVRVCSVSPAIFDTPMLARAVAEAPDPEAYFSVQDE
                     GYPLGRIGRPPELAAAVAFLASDEASFLTGRTLVVDGGVTA"
     gene            complement(4933..5733)
                     /locus_tag="VFZ86_15165"
     CDS             complement(4933..5733)
                     /locus_tag="VFZ86_15165"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006466914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX5643656.1"
                     /translation="MSDAGGADGRCRRFEGRVALVTGGASGIGLATAERLLAEGAAVV
                     IADVDGAAGEAAAAALRGRGLELASAARCDVTRGAEVKALVEGVVARHGRLDVLFNNA
                     GIFEPGEVHEVDEEAWDRQLGVNLRGVYLVAHHVVPVMLARGGGAIVNNASVAALVGD
                     RASAAYCASKGGVALLTRAMALDYAQRGIRVNAICCGEIDTPLFERESYQIGMTPDSY
                     RDLLNEEHPIGRIGLPSEAAAAVAFLASDDASFVTGVLLPVDGGYAAQ"
     gene            6031..6807
                     /locus_tag="VFZ86_15170"
     CDS             6031..6807
                     /locus_tag="VFZ86_15170"
                     /inference="COORDINATES: protein motif:HMM:NF039825.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5684 domain-containing protein"
                     /protein_id="HEX5643657.1"
                     /translation="MDYNYDYDVNDGALGALFAVYLIVMIAIYVVTVVGLWKMYVKAG
                     KPGWAAIIPIYNWWVWVEMIGRPRWWFWALVASVLLSWIPVVGVILGILMFVLYLMGC
                     LDMAKAFGRGAGTGIGLWLVPFVFAPILGFGDAQYQGPVASPAGGGFGAAPPPPPPTG
                     GYGLPPTPPAPPSAPVTPAQTGIPAAPVTPPVTPAAAVPEPPAVPAEAPATADVGPAA
                     PDPGTAATDVGPAAPEDEPDAPEAPQAPPASSPPPPPPLV"
     gene            6994..9270
                     /locus_tag="VFZ86_15175"
     CDS             6994..9270
                     /locus_tag="VFZ86_15175"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX5643658.1"
                     /translation="MRLSTTSLARASASRPWLTLALWGAALAAAVAAIVLVLPGSLTA
                     QYSFIGDPDSQRGRDLLQERLDMPQKANEVVIVRSDTATATDPEFRAAVLAIQRDITA
                     LGPDAVDGAVSAFRGGDETLISADGRTAIIPVVMAGGLTEAEDNIDKVHAVVHAADGR
                     GGFDALVTGAASINSDFSQTAESDLRKGEGIGVPIALVILLIVFGAVVAAGLPILLSL
                     LAITMAIGLTALVGQTFDVSVFAINMVSMMGLATGIDYSLFIISRFREERARGRDKLD
                     AIAVTGGTASRAVLFSGLTVVLALLGLLIVPTNIFASLAIGAILVVSMSVIAALTLLP
                     AVLSLLGDRVNSLKLPYLGRRILDNRSAGRTSWIARVAYRAMKRPALALTVGVGVLLL
                     LAYPALDMKTGVSGVNTFPNDFESKQGFAVLEQQFSAGGVNPVEIVVDGQVDDPAVQA
                     AMTRLTQDLGDDPAFGPVQTQTAKSGNLALLSVPVNGDAVGDLALDKVRELRGTLIPR
                     AFAGSGASVYVSGQTAGNLDYIDIVNRYFPWVVALVLSLSFLLLLVAFRSLVIPLKAI
                     LMNLLSVGAAYGLVTLVSLKGWGASILGFQQVPVVEQWVPLLLFAVLFGLSMDYQVFL
                     LSRIKEAWDRTGDNTLAVTEGIGATAGIITGAALIMVAVFAGFAAGDLVMFQQMGFGL
                     AVAVLLDATLIRTILVPSAMKLLGRWNWYLPSWLEWLPHLSIEGAPAAPAAAPAPADP
                     GTPGGPGLEPVPAAERAA"
     gene            complement(9342..10820)
                     /locus_tag="VFZ86_15180"
     CDS             complement(9342..10820)
                     /locus_tag="VFZ86_15180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5643659.1"
                     /translation="MALIKRITAEDAHRRRWQLLALTSVGAFMWPLDGSIVSVALPVM
                     GPDLHLSFTAAVWVSAAFLLTTAVLLIPAGRIADQRGRVRFYLLGIAVFTAASLLCAL
                     STTGTWLIVSRIIQGGGAALVGATSAAIVTAVFPPRERGRALGINVMAVYIGLTVGPP
                     LGGFIADSVGWRWIFLINIPIGVVVLLWGWFLLPRSEKAPGPRLDVLGSVLLGAFLIC
                     LLVPLTFSVEWGWGSPATIGLLLVSAAALVAFVVWERRVASPILDLDLVLKNRLFAAA
                     NTAALLNYMALYGVSLLTAIFLQLVQEESATVTGWLLLSMPLLMAVLSPFSGRLSDRI
                     GSRVLATGGMLAIAAGMVLLALTPEPAPLWRVAASLAVVGVGMAAFSAPNTSAVMGSV
                     RRDQLSQAGAFLGTMRTAGQALSVALLGGIAASQLGRVGGRLLLTHGHGGGELAARAV
                     DAYAAGYRYAMLTGAALALAGAAVSLTRGAHDPASTPAAAHD"
     gene            complement(10842..11510)
                     /locus_tag="VFZ86_15185"
     CDS             complement(10842..11510)
                     /locus_tag="VFZ86_15185"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HEX5643660.1"
                     /translation="MEFESVVRGRRSIRAYTDQPVPADLVREILDEARWAPSSRNTQA
                     WSVWVMTGEALERFKAAFKAALAREEADAPDLPGTATEDWPQTCSARAVELMKTRAET
                     LEAAGEVAGPAASMARMAECFGAPVLLVFGFEGCLSPAYAAYDTASLVQNVCLAAHDK
                     GLGTCQSTTLVRYPGLLRQLLPGAEEQRLVVAVTLGYPDLTAPANTFARSRAPLDEVV
                     TWVS"
     gene            11763..>12198
                     /locus_tag="VFZ86_15190"
     CDS             11763..>12198
                     /locus_tag="VFZ86_15190"
                     /inference="COORDINATES: protein motif:HMM:NF014646.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HEX5643661.1"
                     /translation="MNETLTSALVEMREDDVLAAVRAAKEAGAALADIIAALQAGMKI
                     IGDKYESEEFFLSELIMSAEIFKNALAELGLDKSAADTGTRGTVVLGTVHTDIHDIGK
                     NIVVSVLSANGFKVVDLGVDVPAQKFVEAVQESGARVVGLSCL"
BASE COUNT         1723 a         4541 c         4303 g         1631 t
ORIGIN      
        1 ggtactcatc ttcgtcgtct cgctgctgcg gccggtgcga cggttctacg gcgtcggcct
       61 gccgggcgaa gccgacgcct gagcccctgc caccgcggac aacacaggtt cctgcaggcc
      121 cgcggcgaac atgccgatgc atgacgggta gcgaagccta caggagcttc agtgcgggca
      181 gagacgaaac gacagcgcgt gttcctcacg ttcgccgcgg gcgatctcgc ccaggcgcgt
      241 ccggtgaccg cccgcctcgt cgccggcggg ggcctcgccc tcgatcaggc gctgaccagt
      301 gagcccttcg cgacggtccg cggcgacatc atccgcgcga gcctgctgac ccgcctgcgc
      361 cactgcgcca gcgcgctctg cctgtacggc gcccgcaccg tggatgacga ctgggtgtgg
      421 tgggcgctcg agacggccgc cgagctcaag ctccccctgc tcggcgcggc tctgcccggc
      481 gaggacgcga aggatgccga gcgcctgctc acgcgcctcg gagcggagat cgtgccgctg
      541 aacggcgagg ccatcgtggc ccgggcgcgc gccgcgtcct cgttcccggc ggcgcgcgcc
      601 atcgacgccg cggcgatcgc ggagacgctc cacctcatgc gtcacccgct gcgctagcgc
      661 ggccacccgt gctccagccg ccggtcgtca tcactgccgc cctgaccggc tcccgcatca
      721 ccagggagca gacgccggcc atccccgtca ccccggagga gatcgccgcc gagggcatcg
      781 ccgcctggcg cgccggggcg gcgatcgtcc acgtgcacgt gcgcgacgca gccggacgcg
      841 gcgcccagga catggacgcc ttccggcgcg tggtgagcgt cctccgggca gagaccgacg
      901 tcgtcctgtg cctcacgacc agcggcatcc ccgggaggaa cctcccgacc gccgagcgca
      961 tcgcgccgct cgcgctgcgg ccggagatgg cgagcttcga cgccggcacc atccgcatgg
     1021 acgccggcat gttcgtcaac gagcccgagt tcctcgacgc gctcgcccgg gaggcgcggg
     1081 agcagggcgt cagactcgaa ctcgagtgct tcgacctcgg catggtggag acggcgctgc
     1141 gctaccacgc tgcgggcaag ataccggcgc cgctgcactt ccagttcgtc accgggacgc
     1201 gctacggcat gccggcgacg gcgcaggcgc tggtcgccgc cgtggacctg ctgcccacgg
     1261 acgccacctg gtcggtgatc ggggtcggcc gcaaccagac gcccatggcg atgctggcgc
     1321 tggccatggg cggacacgtg cgcgtggggc tggaggacaa catccactac cgccgcggcg
     1381 agctcgcgac cggcaacgcg cagctcgtgg agcgtgcggt gcggctggcg agggagatcg
     1441 agcggccggt ggctacgccc gaccaggcgc gcggcatcct cggcctcagg tagagtctga
     1501 tcctcaggta gagcctgagc ccctggcggg gtccgagccc gcgacggagc ctcggcccgc
     1561 gaacccatcg gcgacggcga gcacctcgtc gaggatggcg aggccggtgt cgagctcgtc
     1621 gctccccacc accagcggcg gcgcgatcag cagcaggttc ccgtgcacgt acaagtggag
     1681 gccgcggtcc atggcggccc tccggagggc cgccatcggc tgcgcggcct cacccgtcgc
     1741 ggcgtagggc acgagcggct cgcgggtctc gcggtcgcgc accagctcga gaccccacag
     1801 cagaccgagc ccacggacct cgccgaccga ggtgtgatcg tcggcgagct ccagcagctt
     1861 ggcggccagg atctcgccca tcaccgccgc gttctcgact agccgctcct cgcgcagcgt
     1921 ctccacggtc gcgaccccgg cggcgcatgc gagcgggtgg ccgcaggcgg tgagcccgct
     1981 cgcgaactcg tgctcctcca gccacgggcg cagcttctcg ctcacgacga cggcgccgag
     2041 cggcgcggcg ccgccgctga tgcccttggc cagcgtcatg atgtccggcg tcacgcccca
     2101 gtgctcgcag gcgaaccagc gcccggtccg gccgaagccc gtcatgacct cgtcgaggat
     2161 gagcaggatg ccgtggcggt cgcacacctc ccggatggag gcgaggtagc cgggcggcgg
     2221 cacgaggacg ccgttggcgc cggtcacggt ctcgaggatc acggcggcga ccgaccccgg
     2281 gccctcgaac tgcaggatct cctcgaggtg cgggccaccc gcagcgatgg cacagtcgtc
     2341 gacgtggccg gccgtggggt cgaacgcgtg tgctccgagc gcatcgtagg aatgcccggc
     2401 ggagcagcgg tacgggtagg gatcgaacat gcgcaccacg cccggggcgg cgggttccgc
     2461 cggccagcgc cgcgggtcgc cggtgagggc ggcggcgccg gccgtggcgc cgtggtagct
     2521 gcggtagcgg gcgacgacct tctgacggcc ggtcacccag cgcgccagct tcacggcgtt
     2581 ctcgttggcg tcggcgccgc cggtggtgaa cagggtcatg ccgaggtcgc ccggcgtgac
     2641 ctcggcgagg agccgggcga gctccgatcg cggctcgctg gcgaacgacg gcgccaggcc
     2701 gtacagggcc tccgcctggc gccgcagcgc ctcgacgagg cgcgggtgcc cgtggccgag
     2761 gttcaggttc acgacctgcg acgcgaagtc gaggtagcgc cgtccctggc tgtcccgaaa
     2821 ccagcagccc tccccgccga cgacgcggac ggggtcgatc ccaccctggg cactccacga
     2881 gtagatgacg tgctcgcgtt ccacagagtc gactctatcg ggtcgcgccg ccgcctgcag
     2941 cgcgtccccg catcgtggcg tcgacctgac ccgggccggc ggagcccccc gtgccacaac
     3001 ccacgcccgc gggtacaagg ggtcaaccga cgcccagaag gaggccgcgt gtccgccacc
     3061 accgaccccg ggatcctcac cggcgacgac acggccgtcg ccgggttcat ccgcagcctg
     3121 cccaaggcgg agctgcacct gcacatcgag ggcacactcg agcccgagct cctgctcgag
     3181 ctcggccgcc gcaacggcat cgccctgccc tgctccaccg ccgaggagtg ccgggcgcag
     3241 taccgcttcc acgacctcca ggacttcctg gacatctact acgccggggt gacggtcctc
     3301 gtctccgagc aggacttcta cgacctcacc gccgcctacg tgcgccgcgt catcgccgat
     3361 ggcgcgcgcc acgtggaggt gtccttcgac ccgcagagcc acgtgccacg cggggtcccg
     3421 ttcgccgacg tggtcggcgg catcacgcgc gcgctgcgtg acggcgacgc cggccacggc
     3481 ctcagctggc gcctgatcat gtgcttcctg cgcgaccggc cggcgagcga ggccatggag
     3541 atgctcgagc tggccctccc ccaccgggac gtcatcgccg gggtggggat cgactcggcc
     3601 gaggccggcc acccgccggg cgagttcgcc gaggtgtacg ccagggcgcg ggcggcgggc
     3661 ctcgtcgccg tcgcacacgc cggcgaggag ggcccgcccg cgtacatctc cgaggcgctg
     3721 gacctgctcg cggtgcggcg catcgaccac ggggtcgccg ccatccaaga cgccgagctg
     3781 cagcggcgcc tcgccgcgga acagatccct ctcacgatgt gccccctgag caacctcgag
     3841 ttgcaggtga ccccggacct ctcgcggcac ccgctcaagc gtctgctcga cgccgggctc
     3901 gtcgtcacga tcaactcgga cgacccggcg tacttcggcg gctatcttct ggacaactac
     3961 gtcgccgcgc agaaggcgct gggcctgacc cgcgccgacc tggcccagct ggcgcgcaac
     4021 tcgatcaccg cttcgctgct tccggaggag cgcaaggcgg agctcgtcgc cgagatcgat
     4081 cgcgtggcgg ccgggggctg aggcgcggga cggctgcgcc cgccgcgccg gcgcccgcga
     4141 cgcgtcaggc ggtcaccccg ccgtccacga ccagcgtgcg cccggtcagg aagctggcct
     4201 cgtcgctcgc gaggaacgcg acggcggcgg ccagctccgg gggccgcccg atgcgcccga
     4261 gcgggtagcc ctcgtcctgg acgctgaagt aggcctcggg gtccggcgcc tcggcgaccg
     4321 ctctggcgag catcggcgtg tcgaagatcg ccggactgac ggagcacacg cgcacgccgt
     4381 cgggcccgtg gtcgatggcc atggcccggg tcatcatcac gatcccggcc ttggccgccg
     4441 agtacgcggc ggcgttgggc tcgccgtgga tgcccgagtc gctggcgatg ttgacgatca
     4501 cgccgctcct gcgcgccgtc atgtggcgca gcgccgcccg gcacatgaag taggcgcctt
     4561 tgaggttcac gcccatgagg cggtcgtact cgtcctcgct catctccgtg gtcggcttct
     4621 cgatccagat gccggcggag ttcacgagga tgtcgaggtg gccgaagcgc tcgagggtct
     4681 cggcgacgag gcggtcgcaa tcggcgacga cggacacgtc gcccagcacg accgcgtgct
     4741 cgctcgcgtg cccgttcgcg cgcgccagct cggccgcatg cgcggcgacg gcctgcgtcc
     4801 tcgccgcgtc ccggccgccc acggcgacgc tgacgccctc ttccgcgaag cggtggacga
     4861 tcgcctcgcc gaggcccgag gagccgccgg tgatcaaggc gaccttgtag gtcagtccgc
     4921 gcatctgtcc tcctactgtg ccgcgtagcc gccgtccacc ggcagcagca ccccggtgac
     4981 gaagctcgca tcgtcgctgg ccaggaacgc gaccgccgcg gccgcctcgg agggcaggcc
     5041 gatgcggccg atgggatgct cctcgttcag gaggtcccgg tacgagtcgg gcgtcatgcc
     5101 gatctggtac gactcgcgct cgaagagcgg cgtgtcgatc tcgccgcagc agatggcgtt
     5161 gacgcggatg ccgcgctggg cgtagtcgag cgccatcgcc ctggtgagca gggccacgcc
     5221 gcccttgctc gcgcagtacg cggcggaggc gcggtcgccg acgagggcgg cgacggaggc
     5281 gttgttgacg atcgcgccgc cgccacgcgc cagcatcacc ggtaccacgt ggtgcgccac
     5341 gaggtagacc ccccgcaggt tgacgccgag ctggcggtcc cacgcctcct cgtcgacctc
     5401 gtggacctcg ccgggctcga agatgccggc gttgttgaag agcacgtcga ggcgcccgtg
     5461 gcgggcgacc acgccctcca cgagcgcctt gacctcggcg ccacgcgtca cgtcgcagcg
     5521 cgcggcgctc gcgagctcga ggccgcggcc gcgcagcgcg gccgccgcgg cctcgcccgc
     5581 cgcgccgtcc acgtcggcga tgacgacggc ggctccctcc gcgagcagcc gctccgccgt
     5641 cgccagcccg atgcccgagg cgccgccggt gaccagggcg accctgccct cgaaccggcg
     5701 gcagcgcccg tccgcgccgc ccgcatcgct cacgccgtcc tccccgcgtc cacctcgaac
     5761 accatctcct tcactgccgc cccccacgat cgccgaccgc cgccgggtcg agcgccgacc
     5821 ccggcctcct cgcttcacca tacgtcgccg gaggcgggcg tcaacgatcg attcttgggc
     5881 aaacccagcg gcccgggatc gttcgcggat gccgcagacg tgcaggttcg gccgcggtag
     5941 ccgatacacc gtgcaggcag gaggcgcgcg ccgcgcgctg aactcccacg ggagcgcccc
     6001 gaaaggcgca cggcatcgag gaggaggtcc atggactaca actacgacta cgacgtgaac
     6061 gacggggcgc tcggcgccct cttcgcggtc tacctgatcg tcatgatcgc gatctacgtg
     6121 gtcaccgtcg tcggtctgtg gaagatgtac gtcaaggccg gtaagcccgg ctgggcggcc
     6181 atcatcccca tctacaactg gtgggtctgg gtcgagatga tcggccgtcc gcgctggtgg
     6241 ttctgggcgc tcgtggcgtc ggtgctgctc tcgtggatcc ccgtcgtcgg cgtcatcctg
     6301 ggcatcctga tgttcgtgct ctacctgatg ggctgcctcg acatggccaa ggccttcggc
     6361 agaggcgcgg gcaccggcat cggcctgtgg ctcgtgccgt tcgtcttcgc gccgatcctc
     6421 ggcttcggtg acgcacagta ccagggaccg gtcgcgagtc cggccggcgg cggcttcggc
     6481 gccgccccgc cgccgccacc gcccacgggc ggctacggcc tgccaccgac gccgccggcg
     6541 ccgccgagcg cgccggtcac gcccgcgcag accggcatcc ccgcggcgcc tgtgacgccg
     6601 ccggtcacgc cggccgccgc cgtgcccgag cccccggccg tacctgccga agcgccggcg
     6661 accgcggacg tcggcccggc ggccccagac cccggcaccg cagcgacgga tgtcggcccg
     6721 gcagccccgg aagacgagcc cgacgccccg gaagcgcccc aggcgccgcc ggcgagctcg
     6781 ccgccgccac cgccgccgct ggtgtagaag ccggacacac accgaccgac ccggacgggc
     6841 cggggctgcg ccgcagcccc ggcccgtccc acgtacggca cccccttctg accttcgccg
     6901 cgttttccgg cacgtgctcg cgggcaggca gtccctaacc agacgaaatc actccggctt
     6961 tgctcccggc ggagccgtca aggaagggac atcatgcgac tctcgaccac atcgctcgcg
     7021 cgcgcgtccg cgagccgccc gtggctgacg ctggcgctct ggggcgccgc cctcgccgcc
     7081 gccgtcgccg ccatcgtgct ggtgctgccg gggagcctga cggcgcagta cagcttcatc
     7141 ggcgacccgg actcccagcg cggcagggac ctcctgcagg agcgcctcga catgccgcag
     7201 aaggcgaacg aggtcgtgat cgtgcgctcc gacacggcga cggcgaccga cccggagttc
     7261 cgggcggcgg tcctcgcgat ccagcgcgac atcaccgccc tcggcccgga cgccgtcgac
     7321 ggcgccgtca gcgccttccg cggcggcgac gagaccttga tctccgccga cgggcgcacg
     7381 gcgatcatcc cggtggtgat ggccggcggc ctgacggagg ccgaggacaa catcgacaag
     7441 gtccacgccg tcgtccacgc ggccgacgga aggggcgggt tcgacgctct ggtcacggga
     7501 gccgcgagca tcaacagcga cttcagccag accgccgagt ccgacctgcg caagggcgag
     7561 gggatcggcg tcccgatcgc gctcgtcatc ctgctgatcg tcttcggcgc cgtggtcgcc
     7621 gccggcctgc ccatcctcct cagcctgctc gccatcacca tggccatcgg cctcaccgcg
     7681 ctggtcggcc agacgttcga cgtctcggtg ttcgcgatca acatggtgag catgatgggt
     7741 ctggccacgg gcatcgacta ctcgctgttc atcatctcgc gcttccgcga ggagcgggcg
     7801 cgcgggcgcg acaagctcga cgccatcgcc gtcaccggcg gcaccgccag cagggcggtg
     7861 ctgttcagcg gcctgacggt ggtgctggcg ctgctcggcc tcctgatcgt gccgaccaac
     7921 atcttcgcga gcctggcgat cggcgccatc ctggtggtga gcatgtcggt gatcgccgcg
     7981 ctgacgctgc tgccggccgt gctgagcctc ctcggcgacc gcgtcaacag cctcaagctc
     8041 ccgtatctcg gccgtcgcat cctcgacaac cgcagcgcgg gccgcacctc ctggatcgcg
     8101 cgcgtggcgt accgggcgat gaagcgtccc gccctcgcct tgaccgtcgg cgtcggcgtc
     8161 ctgctgctgc tcgcctaccc cgccctcgac atgaagaccg gggtctcggg cgtcaacacg
     8221 ttccccaacg acttcgagag caagcagggc ttcgccgtgc tcgagcagca gttctcggcc
     8281 ggcggcgtga accccgtgga gatcgtcgtc gacgggcagg tggacgatcc cgccgtccag
     8341 gcggccatga cgcggctcac gcaggacctg ggggacgacc cggcgttcgg ccccgtgcag
     8401 acccagacgg cgaagtccgg gaacctcgcg ctgctctccg tgcccgtgaa cggcgacgcc
     8461 gtcggcgacc tcgcgctgga caaggtgcgc gagctgcgcg gcacgctcat cccgcgggcg
     8521 ttcgccgggt cgggagcgtc cgtctacgtc agcggccaga ccgccggcaa cctcgactac
     8581 atcgacatcg tgaaccgcta cttcccgtgg gtcgtggcgc tcgtcctcag cctgagcttc
     8641 ctgctgctgc tggtcgcgtt ccgctccctg gtcatcccgc tcaaggcgat cctgatgaac
     8701 ctgctcagcg tgggcgcggc ctacggcctg gtcacgctgg tctcgctgaa gggctggggc
     8761 gcgagcatcc tcggcttcca gcaggtgccg gtggtggaac agtgggtgcc gctgctgctg
     8821 ttcgccgtgc tgttcgggct ctccatggac taccaggtct tcctcctcag ccgcatcaag
     8881 gaggcgtggg accggaccgg agacaacacg ctggccgtga ccgaaggcat cggcgccacc
     8941 gccggcatca tcaccggcgc ggcgctgatc atggtcgccg tgttcgcggg cttcgccgcc
     9001 ggcgacctgg tgatgttcca gcagatgggc ttcggcctcg ccgtggccgt gctgctggac
     9061 gccacgctca tccgcacgat cctggtgccg tcggcgatga agctgctcgg ccgctggaac
     9121 tggtacctgc cgagctggct ggagtggctg ccgcacctga gcatcgaggg cgcgccggcc
     9181 gcgccggcgg cggcgccggc gcccgccgat ccgggaacgc cgggcggccc cggcctggag
     9241 cccgtcccgg cggcggagcg cgcggcttga cggcagccgg gcctgcgcgt gccagcagcc
     9301 gggcttaggc gcgcacggat cgcgcccgac gcgcggcgtc gtcagtcgtg cgcggccgcg
     9361 ggcgtggacg ccgggtcgtg ggcgccgcgc gtcagtgaga cggcggcgcc ggccagcgcg
     9421 agcgcggcgc cggtcagcat cgcgtagcgg tagccggcgg cgtaggcgtc gacggcgcgc
     9481 gcggccagct ccccgccgcc gtgcccgtgc gtgagcagca gacggccgcc cacgcggccg
     9541 agctggctgg cggcgatgcc gccgagcagc gcgacgctga gcgcctggcc ggcggtgcgc
     9601 atggtgccga ggaaggcgcc ggcctggctg agctggtccc ggcgcacgct tcccatcacc
     9661 gcggaggtgt tgggcgcgct gaacgccgcc atgcccaccc cgacgacggc gaggctggcg
     9721 gccacccgcc acagcggcgc cggctccggc gtgagcgcca gcaggaccat gccggcggcg
     9781 atggccagca tgccgccggt cgcgagcacc cgcgagccga tccggtcgct caggcggccg
     9841 ctgaacgggc tcagcacggc catcagcagc ggcatgctca gcagcagcca gccggtgacc
     9901 gtcgccgact cctcctggac gagctgcagg aagatggcgg tcagcaggct gacgccgtag
     9961 agcgccatgt agttgagcag cgccgccgtg ttcgcggcgg cgaacagacg gttcttgagc
    10021 acgaggtcga ggtcgaggat gggcgacgcg acgcgccgct cccagaccac gaacgcgacg
    10081 agggcggccg ccgagaccag cagcaggccg atggtggccg gtgagcccca gccccactcg
    10141 accgagaagg tgagcggcac cagcaggcag atgaggaacg ctcccagcag caccgagccg
    10201 aggacgtcca ggcgcggccc cggcgccttc tcgctgcgcg gcagcaagaa ccagccccag
    10261 agcagcacga cgacaccgat agggatgttg atgaggaaga tccagcgcca gccgacggag
    10321 tcggcgatga agccgccgag aggcggcccc accgtcaagc cgatgtagac ggccatgacg
    10381 ttgatgccca gggcgcggcc gcgctcgcgc ggcgggaaga ccgcggtgac gatcgcggcc
    10441 gacgtcgccc cgaccagcgc ggcgccgccg ccctggatga tgcgtgagac gatgagccag
    10501 gtaccggtcg tgctgagcgc gcacagcagc gaggcggcgg tgaagacggc gatgccgagc
    10561 aggtagaagc gcacgcggcc gcgctggtcg gcgatgcgcc ccgccgggat gagcaggacc
    10621 gccgtcgtca ggaggaacgc cgccgagacc cacaccgcgg cggtgaagct caggtggaga
    10681 tccggcccca tgaccggaag ggcgacgctg acgatcgacc cgtccagcgg ccacatgaag
    10741 gcgccgaccg acgtcagcgc caggagctgc cagcggcggc gatgcgcgtc ttcggcggtg
    10801 atgcgcttga tgagcgccaa agggtcaggc ggcgccccgg gtcacgagac ccaggtcacg
    10861 acctcgtcaa ggggcgcgcg cgagcgggcg aacgtgttgg ccggagccgt gaggtccggg
    10921 tagcccagcg tcacggcgac gaccaggcgc tgctcttccg ccccgggcag caactggcgc
    10981 agcaggcccg gatagcggac gagcgtggtg gactggcagg tgccgagccc cttgtcgtgg
    11041 gcggcgaggc acacgttctg gaccagcgac gccgtgtcgt aggccgcgta ggccggcgac
    11101 aggcagccct cgaacccgaa gacgagcagg acgggcgcgc cgaagcactc ggccatgcgc
    11161 gccatggagg cggccgggcc ggcgacctcg ccggccgcct caagcgtctc cgcgcgcgtc
    11221 ttcatgagct cgaccgcgcg cgccgagcac gtctgcggcc agtcctcggt cgccgtcccc
    11281 ggcaaatcgg gcgcgtccgc ctcctcgcgt gccagggcgg ccttgaaggc ggccttgaag
    11341 cgctccagcg cctcgccggt catcacccac acgctccagg cttgcgtgtt gcgcgacgac
    11401 ggcgcccagc gcgcctcgtc gaggatctcg cgcaccaggt cggcggggac cggctgatcg
    11461 gtgtaggcgc ggatgctgcg gcgcccgcgg acgacgctct cgaactccat ggtggcttct
    11521 ccttcgcttg ccggggaccg ctccagccta caacggcgcg tgcagccgtg cccagtcgcc
    11581 ggggggcgta accttgacat gcgcacgacc accgatctac tctttcacat aatgtctaga
    11641 taaataccga cgcgtgcctt cccctcccgc ctccggtaca cggcagaacc cctttcgtcc
    11701 tctgccccag gcgtaccgac cgcgccgact gcaacccgcc cctcaccagc aaggaggccc
    11761 acttgaacga gaccctgacc agcgcgctcg tcgagatgcg cgaggacgac gtcctcgccg
    11821 ccgtgcgcgc cgccaaggag gccggcgcgg cgctggccga catcatcgcc gccctccagg
    11881 cgggcatgaa gatcatcggc gacaagtacg agagcgagga gttcttcctc tccgaactca
    11941 tcatgtcggc ggagatcttc aagaacgcgc tcgccgagct cggcctcgac aagtccgcgg
    12001 ctgacaccgg cacccgcggc accgtcgtgc tgggcaccgt gcacaccgac atccacgaca
    12061 tcggcaagaa catcgtcgtc agcgtcctct cggccaacgg cttcaaggtc gtggacctcg
    12121 gcgtcgacgt gccggcgcag aagttcgtcg aggcggtgca ggagagcggc gcccgggtgg
    12181 tcggcctctc gtgcctgc
//
LOCUS       DASUMX010000320         2252 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_2139569, whole genome shotgun sequence.
ACCESSION   DASUMX010000320 DASUMX010000000
VERSION     DASUMX010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2139569"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="VFZ86_15195"
     CDS             <1..300
                     /locus_tag="VFZ86_15195"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX5643662.1"
                     /translation="CSRHGGEEFAVLLPNTPGDSAYHVAERVRKTLSGTRYTGLGLPP
                     DVNITISVGVATCPRDATVLDELMELADKALYRAKAEGRDTVVLWGAGERERIRA"
     gene            385..921
                     /locus_tag="VFZ86_15200"
     CDS             385..921
                     /locus_tag="VFZ86_15200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX5643663.1"
                     /translation="MATQRHTGDRLTAAELSAWRGFLRVQGSIFRELDAELVAEHDLP
                     LRSYEVLLLLEDAPHRRLRMSDLSRSALVSPSGVTRLVDRLEREGLVCRERCQEDGRG
                     YYAVLTEPGDSRLREARKTHLAGVRRLFLDRLAGDDLRRLAAYWERLVPGAAGTDAEW
                     RRTAPGTAAASACGDGDA"
     gene            914..2170
                     /locus_tag="VFZ86_15205"
     CDS             914..2170
                     /locus_tag="VFZ86_15205"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002622924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX5643664.1"
                     /translation="MPDVVVVGAGLSGLCCARELRAAGLDVLVVERSDAPGGRVRTDV
                     VDGFQLDRGFQVLLTAYPEARRVLDYERLGLRAFESGALIRRDGGFTRLADPRRHPFK
                     ALEGLGAPGSLLDKLRVVRLRRRLSRHSLNELLTAPQVTTAEALRREGFSGDFVDAFF
                     RPVLGGIFLDASLETSSRLFAFVFKLFSEGEAALPAAGMQAIPRQLADGLPEGAVRYG
                     ATVESAAPGEVVLAGGERLTARSVVVAADGPEAARLTGALEAPAPRAVTTLHYAAGRS
                     PVGEPLLVLNGEGHGPVNDLCVPSDVAPSYAPPGAALVSATVLGIPALTDGQLDAAVR
                     EQLRGWFGTQVQGWRLLRALRIPFALPAQPPAAVSPVERPVRLQDGLFVCGDHRGTAS
                     IQGAMVSGRRAAAAVRGLDVAPSCAP"
BASE COUNT          262 a          847 c          853 g          290 t
ORIGIN      
        1 tgctcgcgtc acggcggcga ggagttcgcc gtgctgctgc ccaacacgcc gggcgacagc
       61 gcgtaccacg tggcggagcg cgtgcgcaag accctcagcg gcacgcgcta caccgggctc
      121 gggctcccgc ccgacgtgaa catcaccatc agcgtcggcg tggccacctg cccgcgcgac
      181 gccaccgtcc tcgacgagct catggagctc gccgacaagg cgctgtaccg cgccaaggcg
      241 gaaggccgtg acacggtcgt gctgtggggc gccggggagc gcgagcggat cagggcgtga
      301 cccgcgccgg cccgccggcg agcgcatctt cagcgtggac cgcccgggta tactgccatc
      361 gaccacccca gcggatggag gtccgtggcc acgcagcgac acaccggtga ccgcctcacc
      421 gccgccgagt tgtcggcgtg gcgcggcttc ctgcgcgtcc agggctcgat cttccgcgag
      481 ctggacgccg aactggtggc ggagcacgat ctccccctgc ggtcctacga ggtgctgttg
      541 ctgctcgagg acgcgccgca ccggcggctg cgcatgagcg acctctcccg ctcggcgctg
      601 gtcagcccca gcggcgtgac gcgcctggtg gaccggctgg agcgcgaggg cctggtgtgc
      661 cgcgagcgct gccaggagga cgggcgcggg tactacgcgg tgctcaccga gccgggcgac
      721 agccggctgc gtgaggcgcg gaagacccat ctggccggag tacgacggct gttcctcgac
      781 cggctcgccg gcgacgacct gcggcggctg gcggcgtact gggagcgcct ggtgccgggg
      841 gccgccggca cggacgccga gtggcggcgc acggcgccgg gcacggccgc cgcatccgcc
      901 tgcggcgacg gcgatgcctg acgtcgtcgt cgtcggagcg ggcctctccg gcctctgctg
      961 cgcgcgcgag ctgcgcgccg ccgggctgga cgtcctggtg gtggagcggt cggacgcgcc
     1021 gggcgggcgc gtgcgcaccg atgtggtgga cggcttccag ctcgaccgcg gcttccaggt
     1081 gctgctcacc gcgtaccccg aggcgcgccg ggtgctcgac tacgagcggc tcgggctgcg
     1141 ggcgttcgag agcggcgcgc tgatccgccg ggacggcggc ttcacgcgcc tcgccgaccc
     1201 gcggcggcac cccttcaagg cgctcgaggg tctgggcgcg cccggcagcc tcctcgacaa
     1261 gctcagggtc gtcaggctgc ggaggcgcct ctcgcgccac tcgctcaacg agctgctcac
     1321 ggcgccgcag gtcacgaccg ccgaggccct gcggcgcgag ggattctccg gcgacttcgt
     1381 cgacgccttc ttccggccgg tcctcggcgg catcttcctc gacgcgtcgc tggagacgtc
     1441 gagccgcctg ttcgccttcg tcttcaagct cttctccgag ggcgaggcgg cgctgccggc
     1501 cgccgggatg caggccatcc cgcggcagct ggccgacggc ctgcccgaag gagccgtccg
     1561 ctacggcgcg acggtcgagt cggcggcccc gggcgaggtc gtcctcgccg gaggtgagcg
     1621 cctgaccgcc cggtccgtgg tcgtcgccgc ggacgggccg gaggcggccc ggctcaccgg
     1681 cgccctcgag gcgccggcgc cacgggccgt gaccacgctc cactacgccg ccgggcgctc
     1741 gcccgtcggc gagccgctgc tggtcctgaa cggcgaaggc cacggccccg tcaacgacct
     1801 gtgcgtaccg agcgatgtgg cgccgagcta cgcgccgccg ggggcggcgc tggtctcggc
     1861 cacggtgctc ggcatcccgg cgctcacgga cggccagctc gacgccgccg tccgcgagca
     1921 gctccgcggc tggttcggga cgcaggtaca gggctggcgg ctcctgcgcg ccctccgcat
     1981 ccccttcgcc ctgccggcgc agccgccggc ggccgtctcc cccgtggaac ggccggtgcg
     2041 gctgcaggac ggcctgttcg tctgcggcga ccaccggggg actgcttcga tccagggcgc
     2101 catggtgtcc ggaaggcggg cggccgcggc ggtcagggga ctcgacgtgg cgccctcctg
     2161 cgccccgtag ccgggccggc gcgtcacacc cggccgagca ggacgccgag gcacatcacc
     2221 gcgaggacga ccgccgtcac gaggacgtag cg
//
LOCUS       DASUMX010000321        28808 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_414862, whole genome shotgun sequence.
ACCESSION   DASUMX010000321 DASUMX010000000
VERSION     DASUMX010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 28808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28808
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414862"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            183..1547
                     /gene="mtbB"
                     /locus_tag="VFZ86_15210"
                     /pseudo
     CDS             183..1547
                     /gene="mtbB"
                     /locus_tag="VFZ86_15210"
                     /inference="COORDINATES: protein motif:HMM:NF021053.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="[dimethylamine--corrinoid protein]
                     Co-methyltransferase"
     gene            1752..2774
                     /locus_tag="VFZ86_15215"
     CDS             1752..2774
                     /locus_tag="VFZ86_15215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5643665.1"
                     /translation="MRLTSRLGALAAVLVLAVVLGVAVAGCGDSDDTSDAGSPSAAAD
                     ATSQAYTIGITQIVSHPALDAAVAGFKEALAEKGFTNVTYDDQNAQGDMATASSIAQK
                     FAGAGLDLVLGVATPTTQAVVKADTTTPIVFTAVTDPVGAGLVTDPDAPSANVTGVSD
                     MLPVEPHLELIKQIVPDVKTVGVIYNAGESNSVFLIDAEKAAAQAMGIEIVEATASNS
                     SEVQAAAESLVGRVDAISVLTDNTAVSALESIIKIAEQNKIPLISGDTDSVKRGAVAA
                     YAFDYKDLGMQAGYQAAAILSGTPIADIPVEYAENLQLSVNAAAATAMGVTIPDELSA
                     EAQNQF"
     gene            2894..3796
                     /locus_tag="VFZ86_15220"
     CDS             2894..3796
                     /locus_tag="VFZ86_15220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014488032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5643666.1"
                     /translation="MQVIIQSALLQGLVYAFMVLGVFITFRVLDFPDLTVDGSLPLGA
                     AVTAALIAGGHSPLTATAAGVLAGLGAGVVTGFLHLLLKSGDSDATNYGPKLLAGILT
                     MTALYTVNLRIMGRSNTPLLGVQVIFDKIGALLSISMVAWNLIIGLAVLAVAVKYLLD
                     WLLHTEFGLCLQATGDNESMIRAMGTNTNITRIVGLGIANALVALSGSLVAQQQGFAD
                     VGMGIGTIVAGLAGVIMGEVLFGVRSISWVLFSVIGGSIVYRLLIAVSLRLGIAPTDL
                     KLLTAALVVIALSVPTVRKKVMHS"
     gene            3829..4608
                     /locus_tag="VFZ86_15225"
     CDS             3829..4608
                     /locus_tag="VFZ86_15225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005380886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5643667.1"
                     /translation="MRKVFFPGTPNEVVALDGIDLSVPAGQFVSIIGSNGSGKSTLLN
                     GVAGTFPLSSGKVLLGGRDVTRQPEYVRSRSVGRVFQDPRAGTAPDMTIEENLAISLL
                     RAKRSGLRMGVTNKRRAVMREQLTRLGMGLEDRLKSGVSKLSGGQRQAMSLLMATIAE
                     PSLLLLDEHTAALDPKVAATIMELTGEIVAERHLTTLMVTHNMELALRFGDRLIMMHA
                     GRIVLDLSSEQKATLKVSDLIEKFHEVAGEAFAVDRLLLDA"
     gene            4637..5545
                     /locus_tag="VFZ86_15230"
     CDS             4637..5545
                     /locus_tag="VFZ86_15230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643668.1"
                     /translation="MSRLAALVLFTVLLTMVLVPVPAAQASGGSSGVDPLVERDGIVP
                     TTLLGRGMVGDWDHVVQVVSWLRYRPPRRRVVYLLGGSATRESVVSESAWAAQLTRMT
                     GRPATTFVCASSCQTFEEDARIVKALPRYRGTVLISVGLSRFNMLHPPALLPRYSIRR
                     TAPVPWYQHHYDLKASFSYAQKRRAVREWMEDRYAVFLARYQARLADLEKVIVACEAR
                     GLRVALLEMPLNLPVVGDDFDEPLATYRSACLELAGKHGIKYIDFLSAIGLQNRDFHD
                     LQHLLPSGRSRWQARLSRELLRKGLI"
     gene            complement(5621..7084)
                     /locus_tag="VFZ86_15235"
     CDS             complement(5621..7084)
                     /locus_tag="VFZ86_15235"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HEX5643669.1"
                     /translation="MAAGAPTRQPLAPLRSDFTLRFHDDAGLEPLHEATLRLLETTGM
                     RFESPQALAILKKAGAKVDEGSDLVRLPRRLVEEALSLAPREFVLGARDPSCDLQLGS
                     GFTYGTTDGCGVEVVDWHTGERRSSTKADLEAITRMQDCLGSISYWWPTVSAGDCGET
                     AQLHEIEAGWNNTSKHLMGMVQGEPLARAAVEMATAVAGGAAELRRRPVMSDLIGTVS
                     PLLHDKDGIEAGLVFAAAGVPVCYVTMPNLGTTAPATKAGAFVVGAAEIVAAAVLHEL
                     AAPGAPVIGSIMQIYADPRTALTLTSPLDDRCRFLATELLHSFGLPALGAFGGTDAQG
                     PGTWLAGAETMLQLVQAPLDGCELTTGIGLTNTYQLFTPENLILDDDLYHRARHAFLD
                     IPMDEESLALDVIEAVGPGGHFLAHTHTRAHMRDAVLRSVSQEIGPDGQHFRDPVEVA
                     RERALDILEHYRPEPLAGDVSGELRRLVAAADAAVRR"
     gene            complement(7084..7710)
                     /locus_tag="VFZ86_15240"
     CDS             complement(7084..7710)
                     /locus_tag="VFZ86_15240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HEX5643670.1"
                     /translation="MNDQLKQAIIDGNEAVALAMTQALVATGATPREILDEALLPGMD
                     VVGARMREGECFIPEVLLSARVMQACLDILRPLMAAGDEAGMGTVVIGTVEGDLHDIG
                     KNLVGMLLQGAGFTVVNLGTGVTASDFVAAVKEHQPRIIGMSALLTTTLPHMSETIAL
                     LKDEGLRDAVKVMIGGAPVTAAYAAEIGADGYGANAGMAVERAKELVG"
     gene            complement(7707..9203)
                     /locus_tag="VFZ86_15245"
                     /pseudo
     CDS             complement(7707..9203)
                     /locus_tag="VFZ86_15245"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
     gene            9428..10090
                     /locus_tag="VFZ86_15250"
     CDS             9428..10090
                     /locus_tag="VFZ86_15250"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX5643671.1"
                     /translation="MPRFDTLTSIHKAIRAMVYEEGGALQTTDFADRQEAARAAAHLE
                     TVLFLLHDHHTTEERFVFPKLRPFEEQLVDAMLAQHREVVRLLGATEEARMLVAAEDA
                     GDRVAAGGELNRRFNEMIAFYFDHLAREELEVLPATWRHFDDVELMAIQAAIVSEAEP
                     DVAFQWLAWMFKGLNRPELVGLLSGARLAMPPEALEAVRGLGAASLEPAAWETVREQS
                     GL"
     gene            complement(10107..11324)
                     /locus_tag="VFZ86_15255"
     CDS             complement(10107..11324)
                     /locus_tag="VFZ86_15255"
                     /inference="COORDINATES: protein motif:HMM:NF016328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HEX5643672.1"
                     /translation="MDVQRVYDDGLCMQCGTCAGICPTGAVRLDWDLRAGHRLRVDRA
                     ACNDCGACVEACPGPGVDFRATAWWREHAQDAPCRDFLGPWRRLWFGWAADPAVRHAG
                     SSGGVATALLAGALEEDAADAVLAVGLDAENPLRAVGVVCRTPHEVAACRGSKYNVVA
                     VNRLLRHVLDEPGRYVLVGLPCHIQGLRLAQRRSRRLRERVVFALGIFCGLTNEPRAT
                     EVAALQAGIAPDELAGVSYRGPGWPGGMRLQARDGRVRRRAYPDYYDRHLAALVPPRC
                     RLCPDALAEMADVSVGDAWLERYEGSDGVSDVIVRTPAGERLLDDLAGTLELLPASPD
                     EMVASQTETYRVKRDVLRGRLWLHSLAGRPLPDYPGLDLTASPRDRWLGVAGAAQERL
                     SRYLADRCYPPTR"
     gene            11414..12883
                     /locus_tag="VFZ86_15260"
     CDS             11414..12883
                     /locus_tag="VFZ86_15260"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HEX5643673.1"
                     /translation="MQHAPAVREPATTSEGRVIMNAARLRIWDESACRRVHEATLALL
                     AETGIEVKYAPAAEVYARAGAQVDGTRVRIPPELVASALKSAPRDWTLKPRGGDTLPI
                     VLDAHHSYCGTGSDVLYVCDPDSRERRRVRRADVEGMAALCEKLPNIDFVMSMGLPED
                     APQAVDDLVQVDAMLRGTRKPLIVAPRDGHILAKMREMAALAGEAGSFGIYAMPVPPL
                     QLDEDGASKVIACAELSIPMIWAPAPNAGTTAPASISAVIVVANAEVLAGLVLSQAVR
                     SGAPFVWGVGVGAMNMRTMNEVYSSADIFRGHQAQTDLARWYDLPSFAYAAHTDSKLL
                     DEQWSAEAALTAILGELSRATLLHDVGYLECGLQSSYESIVFGDHLLGWARAFMAPLP
                     VDDEALALGEIEAVGPGGNHLARPYTRRHFRQIWQSDLFDTTRHDAWEAEGSKTLLDR
                     LKERVAELRSEPRAFELPDAAKSGLDRILADVGAHRPGS"
     gene            complement(12939..13616)
                     /locus_tag="VFZ86_15265"
     CDS             complement(12939..13616)
                     /locus_tag="VFZ86_15265"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEX5643674.1"
                     /translation="MDRAHSTERPGKSPQSQQNRRQLSAAASKDRLIEATILCMANYG
                     PAGVSIERITDAAGVSRGLVRHHFGSKRQLLLEAFQRLADGERRAFSAGPAEDDDPVA
                     TLRAIVATEFREELAAPERAHAWFGFWQAALGDPDLKEINELVYAEERERYSELFREA
                     ARRQGLDIDPRRAGIGLVALADGAWIELLMAATDFSVDEALAVCDEYIDMILERGRSG
                     RGRAAGD"
     gene            13680..15194
                     /locus_tag="VFZ86_15270"
                     /pseudo
     CDS             13680..15194
                     /locus_tag="VFZ86_15270"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
     gene            15206..16618
                     /locus_tag="VFZ86_15275"
     CDS             15206..16618
                     /locus_tag="VFZ86_15275"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HEX5643675.1"
                     /translation="MSAAEKDALHQRVLHVLEHVGIGVGSTVALDLLADAGACVDRER
                     RAATLPPELVESCLQKAPASVRLAARDPAHDLLVGVGSPLACCTDGMATMVFDDVTTK
                     VRDATRDDLVYYYGLYDALEELDFIWTSLTLGDRDPLRVGLETDLIALESTSKHLQSV
                     VAHSPEQVPPLLEMLEAIAGAPLAERPIYSSLHCPVSPLRFEGEKLDASIELARHGVP
                     ILIHPLPLLGTTAPMSVLGTTVITLAEILAAVTIFQLAAPGCALMVVAIGGCAHLRTG
                     GFLCGTPEVALVDMAGLAMTRQYGLPSMSGGMLSDAHAADFQAGSEGALLAATSVLCG
                     ADLLTGAGLVNSDMAVSTAKAVLDCDTIGSVKRLLEMPDAVDDASTLIEDIAAVGPGG
                     QFLARRSSRENSRRGEIWCPSVFQRDGLSAHAGRSLVSDALERAAALLAAHAPTPIPD
                     DACRQARAAFERYVRSAGSR"
     gene            16636..18024
                     /gene="mtbB"
                     /locus_tag="VFZ86_15280"
                     /pseudo
     CDS             16636..18024
                     /gene="mtbB"
                     /locus_tag="VFZ86_15280"
                     /inference="COORDINATES: protein motif:HMM:NF021053.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="[dimethylamine--corrinoid protein]
                     Co-methyltransferase"
     gene            18255..19610
                     /locus_tag="VFZ86_15285"
     CDS             18255..19610
                     /locus_tag="VFZ86_15285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013012456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX5643676.1"
                     /translation="MTATMDRERLSALMAREDEDFIARHPKSRELFERAQAHLLDGVP
                     MNWMTRWSSPFPPYVAEAHGARFTCVDGHDYIDFCLGDTGAMTGHSPEGSVAAVAAQA
                     ARGITTMLPTEDSLWVAEELSRRFGLRYWQLALTATDANRFAVRLARHVTGRPKILVY
                     NYCYHGSVDETIITINEGVPGPKPGNAGPPVDPTVTSKVIEWNDLEALARALEPRDVA
                     CVLAEPALTNIGIVLPDEGYHAELRRLTRETETLLVIDETHTICAGPGGCTRAWGLEP
                     DVVTLGKPIGSGVPAAVYGFDQTVADLIHADWDYHAADECGIGGTLAGNMLSVAAVRA
                     TLGEVLTQEAYDRMIPLAGRWADGVQSVIDEFALPWVVKRLGCRAEYWPRPVPPRNGG
                     EAAAAEDEQLDRYLHLFMLNRGILMTPFHNMALMSPQTTAADVDRHTEVLREAARTIT
                     G"
     gene            complement(19620..21095)
                     /locus_tag="VFZ86_15290"
     CDS             complement(19620..21095)
                     /locus_tag="VFZ86_15290"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HEX5643677.1"
                     /translation="MRIDPIRPRLRWRVLGEADVDRLEQAILWTLAEVGVRFPLERAL
                     DALQRGGCPVDRASRIARLPQDVVRAALDAAPRAPLLAARDPRCDIVLDGSACHLSND
                     GCGVRVIDPETGRMRPSTRQDVADSARFVDALPCVSFYWGPVVTAEDVPLSTRPLHEA
                     EAVFSNTSKHFQAVDVVGEDIARRVVEMARAVAGGGEELRRRPIMSLIACPIDPLGNE
                     AVSLEAALVCAEAGVPVGFLSLTLACASAPATMAGNLVVNLAAVLAGIVLLQLAHPGA
                     PVFLAGAPSVMDLKTGGYTGGSPEDYLLAAAATQMAHHFGLAMNMGTLASGAKEPGWQ
                     AAVDDALSTLASVSAGAEMMSGCGLLDGSKTLSYAHLLMEAEVYGIVKRMGGGIEVSD
                     ETLALDVIHRVGPNGTYLAEKHTRAHMGEIWRPGVWDRTSYDAWLGAGKRGALENAEE
                     KARGILRTHAPEPLADDVRAGLRRLVAAADADLVGRGSERG"
     gene            complement(21540..22334)
                     /locus_tag="VFZ86_15295"
                     /pseudo
     CDS             complement(21540..22334)
                     /locus_tag="VFZ86_15295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008810699.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
     gene            complement(22336..23619)
                     /locus_tag="VFZ86_15300"
     CDS             complement(22336..23619)
                     /locus_tag="VFZ86_15300"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX5643678.1"
                     /translation="MHRRAGFVSVGLLAALLALFFTALTPVALATDGASPSPGEEAAA
                     APEGTLIVLGVIPDPSGTRTKWSGDQININLDLNGTDLDAAASVLTVDGEEIVLSEPA
                     SMIELSPGNPTLQMKWTEPTFAQGDFQFQVTLFDTAGTQLQVYDERTDETSDVYAWEF
                     ASEGGASGGSLINFEVMQEWFWYIAAGAIVTVELTIISILLACVFALFGSLGRLSKKM
                     TFKQAWDRYQSREYMFRMVIGRIPYWLATFYTSLFRGTPLLLQIIVIYQGVPEFIRAF
                     GLPDTYNPPAFWSGVAALSLNYGAYLTEVFRAGIQAVPKGQNEAAWAIGLSGWQTQRR
                     IVLPQAFKIVIPAVGNDFIALIKDTSLVSVITVEELLRRAQLAGASSLNNFSTLLVAA
                     AFYWALTIFFSFWQAKLETRMARDKAREKEKGKAK"
     gene            complement(23730..24629)
                     /locus_tag="VFZ86_15305"
     CDS             complement(23730..24629)
                     /locus_tag="VFZ86_15305"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEX5643679.1"
                     /translation="MRKGKTYILVIALLVAVIALAGVLAACGEDEPATEETATATEAP
                     AIEGSAMERATAILGKEPTGLALDIVTKGEVVVANDSNYAPQSYLDPDTNEIIGFDVD
                     VAKGMAEILGLTIKWEHPAWETIPGGLNNGRYDVSIGSMTITPERQETLDFSDPYYYT
                     SGQVFVKKGGTQIAGVEDLAGMKVGVGAQTTYYDFLKKESEADVKTYTTDVDAFPDLL
                     NGNLDFVMTAGPTGQQAILEGKAMEFSGKPLYYEDLAMAVKKGEADWVALLTYSVQQM
                     HENGSLTEMSKQWYNGIDLTVKE"
     gene            complement(24855..26234)
                     /locus_tag="VFZ86_15310"
     CDS             complement(24855..26234)
                     /locus_tag="VFZ86_15310"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HEX5643680.1"
                     /translation="MTENLMHTIGSAGRLAILSQDDLERLDAAALAVLAQTGVAIPSE
                     RARAALVAQGATADGTRITFPPELVRRLVDVAPAHMTLGARAGAALITGERSLVTTDG
                     CCVEIYDLETGEKRGTTADDVATISRVVDALPEIDFCWPAVSAQDRPVETRGLHELYL
                     AVANTGKHVQTVTVVEPELAGVAVEMALTVSGSEERLRAEPPISALLGTVTPLGNDEG
                     TLEAGLVFAEAGVPIGFVTMPMGGSTTPITMAGSLVVGVAEALSSVCAIQAVCPGAPV
                     FICFIPSVMDLKSGDFTGGAPEDTIMAAAVGDVGHFYGLPTQCGVNSSGAKEPGWQSA
                     LDDTTTTYLSLAAGVDMLTGVGMVSGGRIFSYEEMALGVDTLTHARTIAAGVDLTAAG
                     GSAGGQAPPAAGPSLAVDKTHRHWTAGGRETAIDRAHERVAAAVAEHRPPALDPAVDE
                     ELRRLAGIG"
     gene            complement(26237..26710)
                     /locus_tag="VFZ86_15315"
     CDS             complement(26237..26710)
                     /locus_tag="VFZ86_15315"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEX5643681.1"
                     /translation="MRSGDDVHGELAAKNIPHEILHLPSSSKTAQLAAEALGVDVGDV
                     VKSLLFMLDERPVLALVTGDATVDADGLARESGAAQVRLARGQEVREHTGYAPGAVSP
                     CALSTQVPVIADPGVFVPEVVYCGGGTTSTMLKIRSADLAALLRPRTLSIAKWRT"
     gene            26785..27558
                     /locus_tag="VFZ86_15320"
     CDS             26785..27558
                     /locus_tag="VFZ86_15320"
                     /inference="COORDINATES: protein motif:HMM:TIGR01076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class E sortase"
                     /protein_id="HEX5643682.1"
                     /translation="MDQSHRDEVPAPSDADTPPRELPARSVRSRRLRLLGDLLLIAGG
                     LVLAYPFWSAAYAQVQQSRLDTTYRAQTAAFVEDIHANVDTKPRGVTDEELVRRLAVR
                     YRRGLKAGDPIGRLRIPRIDLDRLVQQGVGGRLALDPDGDRSLLRSGPVHYAVTPLPG
                     LGEPFAVAGHRTTYGAPFFKLDRLRKGDRIFVDTPYGKFRYAVAKTTTVDPSDIGVLR
                     DRGYGLVLTTCTPPYSAEHRLIVWAKLERARPPRAARDR"
     gene            complement(27587..>28808)
                     /gene="glp"
                     /locus_tag="VFZ86_15325"
     CDS             complement(27587..>28808)
                     /gene="glp"
                     /locus_tag="VFZ86_15325"
                     /inference="COORDINATES: protein motif:HMM:NF045515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEX5643683.1"
                     /translation="LDAVLAAVRPLPSEEVPLLQALGRAAAERIVSPEQVPTFANSAM
                     DGFAVSGAELEAGRREFQVVVDIPAGRYVAEPVAPGNAARIMTGAPLPPGADTVVQVE
                     HTSVDDDLVTVETAPPSGANVRREGEDVSAGDVLFERGSRLGAAEIGLLAAVGIERVR
                     VARRPRVAILATGSELVGAGRQLAPGQIRNSNSFTAYGQAVAASADPILLGIARDDPD
                     ETRRLLAAALENDVVVTSGGVSVGDYDFVKQVQDELGVERRFWGVATKPGKPLAFGTR
                     NGTLVFGVPGNPVAAMVSFEMYVRPAILALQGRPDIYRPWFYAASAEPVQRTKSRTEA
                     RRCRLTHEGGALRFTTTGPQGSGILSSMAGAEGLFFVPPQYPGGEVGEELLVMLLSGS
                     ASERPAYPVAPAVR"
BASE COUNT         4353 a        10051 c        10102 g         4302 t
ORIGIN      
        1 agcatcggtg gcgcaccccg aacgccgggc ggtagactgg cgcgcggagg cccgccgtac
       61 agtggtcgca tcgcttcgct ctcatcgtgg tagctggcgg gcctcgcacg ctccacggcg
      121 caggcggtcg atccggacga accacggagg tgatgctgtt ggagcccaag atccccacgc
      181 gcatgggtga cgggtcgctg gtcgagctga cccgctccga gatcaaggcc gatctcgagg
      241 aaggcacgca gatcgcggcc aagaaggcca aggttcccga actcacgccg gacgagctcg
      301 atcacctgct cgacatctac gcctcgccgg cgcgcttcac cgccgtcgac atcggcgccg
      361 aggtcgtgct cagcaacgac ggcaccggga ccaagcacat cggcaaccgc gtgcaggacc
      421 tgcagagcta cgagaccctg ctcggcgtcg acatccccga gctgtggcag gccgactact
      481 cctacaaggc ggtcaagacg aacgtggccc acgagggcca gtcgatgaag atcgcccagg
      541 cgctgctcac caacccggtg cagtacggcg ccatgcccga cctggggagg tactcgaagc
      601 ccgacggccc catccccaac tggtccgagc tgctgcccat gggcaagatc gacgaggcgc
      661 gcagcgccca ggtcgaggcc tgcgagatgc tggtcgagga catcaacttc gtcgccgacg
      721 ccatgatcga ggccggcgcc gacggcatcg acatggacac gacgggggcg gcgggcgacg
      781 ccgacttcct cgccgccctc accgcctgcc gcatgctgcg cgagcggtac ccgtggctgg
      841 gcatcgagat cggcatggcc agcgagttcg tgctcggcat gcacggccag ctcgagttcg
      901 acggcgagcg cctcgccggc atgtggccca ggaagcagct cgagatggtg accgcggccg
      961 gcgcttccat ctacgggccg gccgtcaaca tcaacaccgg caagtcgacc gcctggaaca
     1021 tcgcccgggc gatcaccttc atcaagccgg ccgccgaggt cgccacgatc ccggtgcacg
     1081 tgaacgtggg catgggcgtc ggcggcgtgc cgacctgcct gttcccgccc gccgacgcca
     1141 ccagccgggc gagcaaggct ttcgtcgaga tcctcaagat cgacggctac taggtgggca
     1201 cgggcgaccc cctcggcatg gagggagccc acacgcaggc gtccggcatg ggcggtatgc
     1261 gcgcggcggg agacctcgtc gcgcggatgc agatgaccag gcgcatgcgg ctcaaggaag
     1321 ccaaggagta cgtggccggc aagctcggct gcagcacgcg cgacctgtcc gacccggtcg
     1381 cgatggagac catccgccag gaccacggcc tcggcctgct caccatgacc tcctccacgc
     1441 acccggcgct gccgggcatg atggaggcca agttcaacat cagcaagctg ctcgacatcc
     1501 cgatcaactc ggtcgagcgc ttcaaggagc ggacctcggg cttctgagcg cctgagcggc
     1561 gcggggaggg gcgggaggcc cgcccccacg acgcgccgcc ggccgcctgc ggccggtcct
     1621 gcgagtggcc cgaaggggcg gcgcgccgag gggcgcgccg ccccttctgc ctgcatactc
     1681 atgagccggc ttgttagcgg gtggtggtga accatacaat gcgccaatca gtcaggttcg
     1741 aaaggagaat catgcgactc accagcaggc tcggcgcgct cgccgctgtg ctcgtcctcg
     1801 cggtggtgct cggcgtcgcc gtcgccggct gcggcgactc ggacgacacg agcgacgccg
     1861 gcagtccgag cgcggccgcc gacgcgacca gccaggcgta caccatcggc atcacgcaga
     1921 tcgtcagcca cccggcgctc gacgccgccg tcgccgggtt caaggaggcg ctcgccgaga
     1981 agggcttcac caacgtcacc tacgacgacc agaacgcgca gggcgacatg gcgaccgcgt
     2041 cgagcatcgc gcagaagttc gccggcgcgg gtctcgatct ggtcctcggt gtggcgacgc
     2101 cgacgaccca ggcggtcgtc aaggccgaca ccaccacgcc gatcgtcttc accgccgtca
     2161 ccgacccggt cggcgccggc ctcgtcaccg acccggacgc tccttccgcg aacgtcacgg
     2221 gcgtcagcga catgctgccg gtggagccgc acctcgagct catcaagcag atcgtgcccg
     2281 acgtcaagac cgtcggcgtc atctacaacg ccggcgagtc caactcggtg ttcctgatcg
     2341 acgccgagaa ggccgccgcg caggcgatgg gcatcgagat cgtcgaggcc accgccagca
     2401 actcgtccga ggtgcaggcc gcggcggagt cgctcgtcgg ccgcgtggac gccatctccg
     2461 tgctcaccga caacaccgcc gtctcggcgc tggagagcat catcaagatc gccgagcaga
     2521 acaagatccc gctcatctcg ggcgacaccg acagcgtcaa gcgcggcgcg gtcgcggcct
     2581 acgcgttcga ctacaaggac ctgggcatgc aggccggcta ccaggccgcc gccatcctca
     2641 gcggcacgcc gatcgccgac atcccggtcg aatacgccga gaacctgcag ctgtcggtca
     2701 acgcggcggc cgcgacggcg atgggcgtca ccatcccgga cgagctctcg gccgaggccc
     2761 agaaccagtt ctgagccgcc gctagtccac gtcctcgcga ccccggcccg cctgcgggcg
     2821 ggccggggtc ggggttgcca agagggggcc ggagcagcga agctactccg gcccccgaca
     2881 cggagtcgcg ccagtgcagg tcatcatcca gagcgccctg ctgcagggtc tcgtctacgc
     2941 gttcatggtc ctcggcgtgt tcatcacgtt ccgggtgctc gacttccccg acctcaccgt
     3001 ggacggcagc ctgccgctgg gagccgccgt cacggcggcg ctcatcgccg gcggccactc
     3061 gcccctcacg gccacggcgg ccggcgtcct cgcgggcctc ggcgccggcg tcgtcacggg
     3121 gttcctgcac ctgctgctga agtcgggcga cagcgacgcg accaactacg gccccaagct
     3181 gctcgccggc atcctcacga tgacggcgct gtacacggtc aacctgcgca tcatgggcag
     3241 gagcaacacg ccgctcctgg gcgtgcaggt gatcttcgac aagatcggcg cgctgctgtc
     3301 catctcgatg gtcgcctgga acctcatcat cggactcgcg gtgctcgcgg tggccgtgaa
     3361 gtacctgctc gactggctcc tgcacaccga gttcggcctc tgcctgcagg ccaccggcga
     3421 caacgagagc atgatccgcg cgatggggac caacaccaac atcacgcgca tcgtggggct
     3481 gggcatcgcc aacgcgctgg tcgcgctgag cggctcgctg gtggcgcagc agcaggggtt
     3541 cgccgacgtc ggcatgggca tcggcaccat cgtcgccggg ctcgccggcg tcatcatggg
     3601 cgaggtgctg ttcggcgtgc gcagcatcag ctgggtgctc ttcagtgtca tcggcggctc
     3661 gatcgtctac cggctgctga tcgccgtgag cctgcggctc ggcatcgccc cgaccgacct
     3721 gaagctcctg accgccgcgc tcgtggtcat cgccctctcc gtgccgaccg tgcgcaagaa
     3781 ggtgatgcac tcgtgaatca cgcgccggaa cccctcgaga tcagcggcat gcgcaaagtc
     3841 ttcttcccgg gaacgccgaa cgaggtcgtg gcgctggacg ggatcgacct gtcggtcccg
     3901 gccggccagt tcgtgagcat catcgggtcg aacgggtcgg gtaagtcgac gctgctcaac
     3961 ggggtggcgg ggaccttccc cctcagctcg ggcaaggtgc tgctcggggg acgcgacgtc
     4021 accaggcagc ccgagtacgt gcgcagccgg tccgtgggcc gggtgttcca ggacccgcgc
     4081 gccggcacgg cgcccgatat gacgatcgag gagaacctgg cgatctcgct gctgcgggcc
     4141 aagcgcagcg gcctgcgcat gggggtgacc aacaagcggc gcgcggtgat gcgcgagcag
     4201 ctcacgcgcc tgggcatggg tctggaggac aggctcaagt cgggcgtgag caagctctcc
     4261 ggtgggcagc ggcaggcgat gtcgctgctc atggccacca tcgccgagcc gagcctcctg
     4321 ctgctcgacg agcacacggc ggcgctcgat cccaaggtcg cggcgacgat catggagctc
     4381 acgggcgaga tcgtcgccga gcgtcacctg acgacgctga tggtgacgca caacatggaa
     4441 ctggccctgc gcttcggcga ccggctcatc atgatgcacg cggggcgtat cgtcctcgac
     4501 ctgagcagcg agcagaaggc cacgctcaag gtgagcgacc tcatcgagaa gttccacgag
     4561 gtcgccggcg aggcgttcgc cgtcgaccgg ctcctccttg acgcctgatc ccgcggcggg
     4621 tggcaagggc tccgtcgtga gcaggctcgc agccctcgtc ctcttcacgg tcctcctgac
     4681 catggtgctc gttccggtcc ctgccgcgca ggcgtccggc ggctcctccg gcgtcgaccc
     4741 cttggtggag agggacggga tcgtgccgac gacgctcctg ggcaggggca tggtcggcga
     4801 ctgggaccac gtcgtgcagg tcgtcagctg gctccgctac cgcccgccgc ggcggcgggt
     4861 cgtctacctc ctgggcggct ccgcgacgcg tgagtcggtc gtgagcgaga gcgcctgggc
     4921 ggctcagctg accaggatga cgggcaggcc ggccacgacc ttcgtctgcg cgagcagctg
     4981 ccagacgttc gaggaggacg cgcgcatcgt gaaggcgctc cccaggtatc gcggcaccgt
     5041 gctcatcagc gtgggcctct cccggttcaa catgctgcac ccgccggctc ttctgccccg
     5101 gtactcgatc aggcggacgg cgccggtccc ctggtatcag catcactacg atctgaaggc
     5161 gtcgttctcc tacgcgcaga agcgtcgcgc cgtgcgtgaa tggatggagg acaggtacgc
     5221 ggtgttcctc gcccgctatc aggcacgcct cgccgacctc gagaaggtga tcgtggcctg
     5281 cgaggctcgc gggttgcgcg tcgcgctgct cgagatgccc ctgaacctgc cggtcgtcgg
     5341 cgacgacttc gacgagccgc tcgcgaccta ccgcagcgcg tgcctcgagc tcgccggcaa
     5401 gcacggcatc aagtacatcg acttcctctc cgcgatcggc ctgcagaaca gggacttcca
     5461 cgacctccag cacctgctgc cgtcgggacg gtccaggtgg caggcgcggc tgtcgcgaga
     5521 gctcctgcgc aagggcctca tctgacgacg cgcggcgggg cgcctcaggt acggcgcccc
     5581 gccgcgtcgc gtcgtcgcgt cctcggctcc gaggcgcagg ctagcgtctg accgccgcat
     5641 ccgccgcagc gacgaggcga cggagctcgc cgctcacgtc gccggcgagc ggctccggac
     5701 ggtagtgctc gaggatgtcg agcgcgcgct cgcgcgccac ctcgacgggg tcgcggaagt
     5761 gctgcccatc tggcccgatc tcctggctca ccgagcgcag caccgcgtcg cgcatgtgcg
     5821 cgcgcgtgtg cgtgtgggcg aggaagtggc cgcccgggcc tacggcctcg atcacgtcca
     5881 gcgccagtga ctcctcgtcc atcgggatgt cgaggaacgc gtgccgggcg cggtggtaga
     5941 ggtcgtcgtc gaggatcagg ttctcgggcg tgaacagctg gtacgtgttc gtcagcccga
     6001 tgccggtggt gagctcgcac ccgtcgagcg gcgcctggac gagctgcagc atcgtctcgg
     6061 cgccggcgag ccaggtgccc ggaccctgcg cgtcggtgcc gccgaacgcc cccagcgcgg
     6121 ggagaccgaa gctgtgcagc agctccgtgg cgaggaaccg gcagcggtcg tccagcggcg
     6181 aggtcagggt gagcgccgtc cgcgggtcgg cgtagatctg catgatcgac ccgatgaccg
     6241 gcgctcccgg cgcggcgagc tcgtggagga ccgccgccgc gacgatctcg gcggcgccga
     6301 cgacgaacgc gccggccttg gtggcgggag ccgtcgtgcc gaggttcggc atggtgacgt
     6361 agcagacggg cacgccggcc gcggcgaaga ccaggccggc ctcgatgccg tccttgtcgt
     6421 gcaggagcgg cgacaccgtg ccgatgaggt cgctcatcac gggccggcgg cggagctccg
     6481 cggctccgcc ggccaccgcg gtcgccatct cgacggccgc ccgggcgagc ggctcgccct
     6541 gcaccatgcc catcaggtgc ttgctcgtgt tgttccagcc ggcctcgatc tcgtgcagtt
     6601 gggcggtctc gccgcagtcg ccggcgctga ccgtcggcca ccagtagctg atggagccga
     6661 ggcagtcctg catgcgcgtg atcgcctcga ggtcggcctt ggtggacgag cgccgctcgc
     6721 cggtgtgcca gtcgacgact tccacaccgc acccgtcggt ggtcccgtag gtgaagccgg
     6781 agccgagctg caggtcgcag gaggggtccc gcgcgcccag gacgaactcg cgcggggcga
     6841 gcgagagggc ctcctcgacc agccggcggg gcaggcggac gaggtccgag ccctcgtcca
     6901 ccttcgcccc ggccttcttc aggatcgcga gcgcctgggg cgactcgaag cgcatgcccg
     6961 tggtctcaag gagccgcagt gtcgcttcgt gcagcggctc cagcccggcg tcgtcgtgga
     7021 agcggagcgt gaagtcggag cgcaggggcg caaggggctg gcgcgtcggc gccccggcgg
     7081 ccatcacccc accagctcct tcgcccgctc caccgccatg ccggcgttgg cgccgtagcc
     7141 gtcggcgccg atctccgcgg cgtaggcggc ggtgaccggg gcgccgccga tcatcacctt
     7201 gaccgcgtca cgcaggcctt cgtccttgag cagcgcgatg gtctcgctca tgtgcggcag
     7261 ggtggtggtc agcagggcgc tcatgccgat gatcctcggc tggtgctcct tgaccgcggc
     7321 gacgaagtcg ctcgcggtca ccccagtgcc gaggttgacg acggtgaagc cggcgccctg
     7381 gagcagcatg ccgaccaggt tcttgccgat gtcgtgcagg tcgccctcga cggtgccgat
     7441 cacgacggtg cccatgccgg cctcatcgcc ggccgccatc agcggacgca ggatgtcgag
     7501 gcaggcttgc atgacgcggg cgctgagcag tacctcgggg atgaagcact cgccctcgcg
     7561 catgcgcgcc ccgaccacgt ccatgccggg aaggagcgcc tcgtcgagga tctcacgggg
     7621 cgtggcgccg gtggcgacga gcgcctgggt catcgccagg gcgacggcct cgttgccgtc
     7681 gatgatggct tgcttgagct ggtcgttcat gcggcgcctt tctcacggtc ggcagctttg
     7741 atgatgcggc gcacctgctc ggcgacgtcc cggtcgatcg gcaggggccg gtgagactct
     7801 acaagttcca gcgcccgggc gcgcgtccgc gtggcgaggt cggtcgcgcc gcgggcctcc
     7861 cagtcctcgc gcacgcggcg gtcgaggacc tccggctggc tgagctcgcg catgtggcgc
     7921 atggtggcgt cgaggctgag gaagtcgccg aaagccccga cggcggcgat gtcgtccacc
     7981 gcgaggctct cgtcgtcgac gcggatgccg ccgacgacgt gcttgatcat gcgcgcgatc
     8041 tcgttgtcca tgaccagctg gccgaggtcg aaggtgacgc ccgactcgat catccccgca
     8101 ccgtagatct ggttggcccc tgccaacgca ggaaggagtc cggtgagggt cttctcgtga
     8161 ccggtctgcg cgtcaccgag ctttgaatcc gcctacaagc ccgccacgaa gctcggaagc
     8221 aggtagcgac gggcgatctg ggcgcaggcg gcggagaaga gggcgagctc cggggagccc
     8281 acgcaggccg ccgccagctt gaggtccatc gccgtggtgg acgacccata ggtgaccggg
     8341 cagccgcgct cgaccaattg cgcgaggacg atgcctccga gcacctcggc gttgtggagc
     8401 acgagcgtgc cggcgagcgt gaccggggag gatcctccgg acatggccat gctgaggatc
     8461 gtgtccgggc accaggcgcg cgccgactcg atgatcacct cggtggcgtc ccgcgggagc
     8521 ttcagcgggc tgaccggaca gacgccgaag ccgatgagcg ggcggcggcg gaactcgtct
     8581 tcgccgccgg cgaccaccgt ggccatctcg atcagctttc tgcagtcgta ggcgctcgtc
     8641 gcctcggtcc cgaccggctt gccggtgttg aggatcgccg cctcccactt gtggaggcag
     8701 gcagtctcct cgggcacgtc gccggggtgc actgccgact cataggtgtc gatgtcgctg
     8761 agggcgtcca ccaggcgcgc cgcctcgcga acgtcctgga gcaccggatg ccggtactga
     8821 ccggtgcgct ggtcgacggt catgatgcct tcgtcgaagt tggagaagta gacgcgacca
     8881 gcctcgagga cgatgtcctg gtccgggtcg cggccgcaga ggtagctctt tggcggggcg
     8941 gagcggatgg cgtcctggac gagatgcgcc gggatgcgga ccatccgagt ctcgcggtcg
     9001 atgaagcagc cgccgtcgct gaagatgtcg agcgcttcgt ccgcctccac ccagacgccg
     9061 gtgcgctcaa ggacctcgag ggagccgagg tggatatcgt cgagctcggc ttccgtgaac
     9121 acggacaggc tcagcccccc tgatctgggc ctcccggagt gcaggttccg cttcactgga
     9181 gacacctccg tgcttggcga catcgctgcg agcgtatggt cgggacgcgc gcgagtaaag
     9241 cgtcaccgcc gcgcgcgcca agttcgccga gcaatccgac gcgtccctcc cgtggcaagg
     9301 cgccaggttc gcagaagttc cactcacccg gcctcggccc ctcggagtca acgccgcggc
     9361 gaccggcaac gcatggctgc ggaagtatcc ttcgggaagc gggaggactc gacgcggagg
     9421 tgggacgatg ccccggttcg acacgctgac atccatccac aaggcgatac gggcgatggt
     9481 ctacgaggag ggcggcgccc tgcagaccac cgacttcgcc gacaggcagg aggccgcccg
     9541 agcggcggcg catctcgaga ccgtcctgtt cctgctgcac gaccaccaca cgaccgaaga
     9601 gaggttcgtc ttcccgaagc tcaggccgtt cgaggagcag ctcgtcgatg cgatgctggc
     9661 ccagcatcga gaggtcgtcc ggctgctggg cgccaccgaa gaggcccgga tgctggtcgc
     9721 agcggaggac gcaggggacc gcgtggcggc aggcggcgag ctgaaccggc gcttcaacga
     9781 gatgatcgcc ttctatttcg accatctcgc ccgcgaagag ctcgaggtgc tgcccgctac
     9841 ctggcgacac ttcgacgacg ttgagctcat ggcgatccag gcggccatcg tcagcgaggc
     9901 tgagcccgac gtggcgttcc agtggctggc gtggatgttc aagggcctca accggccgga
     9961 gctggtcgga ctgctcagcg gagcaaggtt ggcgatgccg ccggaagctc tcgaggcagt
    10021 gcgaggtctc ggcgcggcga gcctggagcc ggcggcctgg gagaccgtgc gcgaacagtc
    10081 cggcctgtag acagccgggc gccccgtcaa cgggtcgggg ggtagcacct gtccgccaag
    10141 taccgggaca ggcgctcctg ggccgcgccg gcgacgccga gccagcggtc tcgaggagaa
    10201 gcagtcagat ccagaccggg atagtcgggg agcggccgcc cggccagcga gtgcagccag
    10261 agccggccgc gcaggacgtc gcgcttgacc cggtaggtct cggtctggga ggcgaccatc
    10321 tcgtcggggc tcgccggtag cagctcgagg gtgccggcga gatcatccag caggcgctca
    10381 ccggccggcg tcctgacgat cacgtcgctc actccgtccg agccctcgta ccgctcgagc
    10441 cacgcgtcac ccacggagac gtcggccatc tccgccaggg cgtcggggca gagccggcag
    10501 cgaggcggca cgagcgccgc gaggtgccgg tcgtagtagt ctggataggc acgccggcgg
    10561 acgcggccgt ccctggcctg cagacgcatc ccgccgggcc agccagggcc gcgataggag
    10621 acgcccgcca gctcatcggg agcgatgcct gcctgcagtg cggccacctc ggtagcgcgc
    10681 ggctcattgg tcagcccgca gaagatgccc agagcgaaga cgacacgctc gcgcagccgg
    10741 cgggagcggc gctgggcgag ccgcagtccc tggatatgac agggcagccc gacgaggacg
    10801 tagcgccccg gctcgtcgag cacgtggcgc aggagcctgt tcacggcgac gacgttgtac
    10861 ttggagccgc ggcacgcggc gacctcgtgt ggcgttcggc agacgacgcc gaccgctcgc
    10921 agggggttct ccgcgtcgag gccgacggcc agcaccgcgt ctgccgcatc ctcctccagc
    10981 gcgccggcga gcagggcggt ggccacaccg ccggacgagc cggcgtggcg gaccgcgggg
    11041 tcggcagccc agccgaacca gagccgccgc catggtccca ggaagtcgcg acagggggcg
    11101 tcctgagcgt gctctcgcca ccaggccgtc gcccggaagt cgacgcccgg gcccgggcac
    11161 gcctcgacgc aggcgccaca gtcgttgcat gcggcccggt cgacccgcag acggtgaccg
    11221 gcgcgcagat cccagtcgag gcggacggcg ccggtcgggc agatgccggc gcaggtgccg
    11281 cactgcatgc agagaccgtc gtcgtacacg cgctggacgt ccataggggg tagcatcccc
    11341 gtctgggagg gactctgtcc agagcgggcg ccgacgagcg gctggcctgt ggcgtcaagg
    11401 gagtgcggcg gcgatgcagc atgcgccggc cgttcgggag ccggccacca cgagcgaagg
    11461 gagggtgatc atgaacgctg ctcgcctgag gatctgggac gagagcgctt gccggcgcgt
    11521 gcatgaagcg acgctggcgc tgctcgccga gaccggcatc gaggtcaagt acgcgccggc
    11581 cgcagaggtc tacgcccggg ccggcgcgca ggtcgacggc acgcgcgtac gcatcccacc
    11641 cgagctggtc gcgtctgcgc tgaagagcgc gccgcgcgac tggacgctca agccgcgcgg
    11701 cggcgacacg ctccccatcg tcctcgacgc ccaccactca tactgcggca ccgggtcgga
    11761 cgtcctctac gtctgcgacc ctgattcccg cgagcgccgt cgcgtccgtc gcgccgacgt
    11821 cgagggcatg gcggcgctct gcgagaagct gcccaacatc gatttcgtga tgagcatggg
    11881 gctgccggag gacgcgccac aggccgtcga tgacctcgtg caggtggacg cgatgctgcg
    11941 cggcacgcgc aagccgctga tcgtcgcgcc gcgtgacggc cacatcctcg cgaagatgag
    12001 ggagatggct gcgctcgcgg gcgaggccgg cagcttcggc atctacgcga tgcccgtgcc
    12061 gccactccag ctcgacgagg acggggccag caaggtgatc gcctgcgccg agctgagcat
    12121 ccccatgatc tgggcgccgg cgccgaacgc cggcaccacg gcgccggcca gcatcagcgc
    12181 cgtcatcgtc gtcgccaacg ccgaggtgct cgccggcctg gtcctcagcc aggcggtcag
    12241 gtccggcgcg ccgttcgtct ggggcgtcgg cgtcggcgcg atgaacatgc ggacgatgaa
    12301 cgaggtctac tcgtccgcgg acatcttccg cggccatcag gcgcagacgg acctggcgcg
    12361 ctggtacgac ctgccgagct tcgcctacgc ggcgcacacc gacagcaagc tgctcgacga
    12421 gcagtggagc gccgaggcgg cactcaccgc catcctcggc gagctctcgc gcgccacgct
    12481 gctccacgac gtcggctacc tcgagtgcgg cctgcagagc agctacgagt cgatcgtctt
    12541 cggcgaccac ctgctcggct gggccagggc gttcatggcg ccgttgccgg tggatgacga
    12601 ggcgctggcg ctcggcgaga tcgaggcggt gggaccaggc ggcaaccacc tggcgcgccc
    12661 ctacacgcgc aggcacttcc ggcagatctg gcagagcgac ctgttcgaca cgacccgcca
    12721 cgacgcgtgg gaggccgagg ggtcgaagac gctgctcgac cgcctcaagg agcgggtcgc
    12781 ggagctgcgc tccgagccgc gcgccttcga gctgccggac gcggcgaagt caggcctcga
    12841 ccggatcctt gcggacgtcg gggcccaccg cccggggagc tgaccggcca ggcaggaccc
    12901 gccgaggacg catactcagg gagccgcacc gtgacagctc agtcgccggc ggctctcccg
    12961 cgaccggagc gacctcgctc caggatcatg tcgatgtact catcgcagac cgcgagcgcc
    13021 tcgtcgacac tgaagtccgt cgccgccatg agcaactcga tccaggctcc atcggccagg
    13081 gccacgagcc cgatcccggc ccggcgaggg tcgatgtcca ggccctgtct cctcgccgcc
    13141 tcgcggaaga gctcgctgta ccgctcgcgc tcctcggcgt agacgagctc gttgatctcc
    13201 ttcaggtccg ggtcgccgag ggctgcctgc cagaatccga accaggcgtg agcccgctcg
    13261 ggtgccgcca gctcctctcg gaactccgtg gcgacgatgg cgcgcagcgt cgccaccggg
    13321 tcgtcgtcct cggccgggcc ggcgctgaac gccctccgct ccccgtccgc cagtcgctgg
    13381 aacgcctcga ggaggagctg ccgcttgctg ccgaagtggt gccgcaccag gccgcgggac
    13441 accccggcgg cgtcggtgat cctctcgatg gagacgcccg cggggccgta attggccatg
    13501 cagaggatcg tcgcttcgat gagcctgtcc tttgaggcgg ccgcgctgag ctgacgcctg
    13561 ttctgttgcg actgcgggct ctttccgggc cgctctgtcg agtgcgctcg gtccatcgcg
    13621 tcggagcgta ccacgccggt cttgacgcgg accagactgg cttgctatac gtacagatag
    13681 tgaaaccact ggggggaggt ggctcattga aacgacgcat ccacgcgggc cgcgcgcgca
    13741 gtggaggctt cagcctgaat gccttcaccg acgacgagtt cagggatctc cacctcgcga
    13801 cactggaagt cctcgagcag acgggcgtct tcaccgacga ccccgaggcg gtggacatct
    13861 tcgagtccgg tggcgcgagt gtcgatcgtg cgaccggggt ggtgaggatc ccgccgcggc
    13921 tcgtcgaggc ggcgatcgac tcgtcgccgg agcgcatcct ggccgccggt cgcagtccca
    13981 aggacgacat catgctcgac gccgggcgcg tcggcttcac gacgttcggc gagggcatca
    14041 gcatcgtcga cccccagacc ggcgagatgc gcgagccgac caagcaggac gtcgccgaca
    14101 ccgcgcgagt cgccgactac ctggacgaac tggatgcgta cgagatcgcc gtcggagccc
    14161 acgacatgcc ggccgcgacg gcgaccatcc acagctacga ggcggcgctg ctcaatacga
    14221 ccaagcatgt ctgcgccggc ccgctctccc gatgggaggc tgaagcgatc ctcgacatcg
    14281 ccgccgccgc cgtgggtggc cgggaggctc tgcggcggcg tccgatcgtg atgatcggag
    14341 tatgtccagt gagtccgctg aagctcccca aggagtgctc ggaggcgatc gtctcggccg
    14401 cgcgtgcagg cgtcccctgc ctggtgctct cgatggctat ggccgggggc tcggcgcccg
    14461 tcaatctcgc cggtacgctc gtcgtccaca acgccgaggt gctcgccggg atcacgctcg
    14521 cgcagctcac gagcccaggc acgccgatcc tctacggcag ctcgacgacg gccatggacc
    14581 tgcgtctcgc ggcggcctcc gtcggctcgc cggagtgtgg cgtcatcagc gccgccgtcg
    14641 ccgccatcgc gcgacagtat cgactgccca gctacgtggc cgggttgtag ggcgactcca
    14701 agctcttcga cgctcagacc ggtcacgaga agacgttgac cgcgctgctg ccggcgttgg
    14761 caggtgccaa cctgatctac ggcgcgggga tgatcgagtc gggtgtcgcg ttcgatttcg
    14821 gccagctcgt catggacaac gagttcgccc ggatgatcag gcacgcggtc atgggcgtcg
    14881 cggtggacga cgagcggctc ggcgtggacg acatcgccca ggtgggcccc tttggggact
    14941 tcctcagcct ggactccacg ctcaagttca tgcgcgacca gtcccagccg aagttgatcg
    15001 accgcagagt gcgggaggac tgggccgccg gcggcggcaa ggacctgcac caacgcgcgc
    15061 tggaggaggc acgccgcatc ctcgcgacgc acgtgccgga gccgctgccg gacgaggtgg
    15121 tgcgcgagat ccgcgggatc gtggacgcgg cggacgccgc cgcggcggca caagggagtc
    15181 cgcgatccac ctgaagttcg actgcctgtc ggccgccgag aaggacgcgc tgcatcagcg
    15241 cgtcctgcac gtgctcgagc acgtcgggat cggtgtaggc tcgaccgtcg ccctggacct
    15301 tctcgccgac gccggcgcgt gcgtcgacag ggagcgccgc gcagcgaccc tgcccccgga
    15361 gctggtcgag agctgtctgc aaaaggctcc cgcgagcgtg cggctcgccg cgcgcgatcc
    15421 ggcgcacgac ctgctggtcg gcgtcggctc gccgctggcc tgctgcacgg acggtatggc
    15481 gacgatggtc ttcgacgacg tgactactaa ggtgcgtgac gccacgcgag atgacctcgt
    15541 ctactactac ggtctctacg acgccctcga ggagctcgac ttcatctgga cgtcgctgac
    15601 cctgggtgat cgcgatcctc tgcgggtggg tctcgagacc gacctcatcg ccctggagtc
    15661 cacgtccaag cacctgcaga gcgtcgtcgc gcactcgccc gagcaggtgc cgccgctcct
    15721 ggagatgctg gaggcgatcg cgggggcgcc gctcgccgaa cggcccatct actcctccct
    15781 gcactgcccc gtgtcgccgt tgcggttcga gggcgagaag ctcgatgcct cgatcgagct
    15841 cgcccgtcac ggtgtgccga tcctcatcca ccccctgccg ctgctgggga ccacggcccc
    15901 catgagcgtg ctgggcacga ccgtcatcac gctcgcggag atccttgctg ccgtcaccat
    15961 cttccagctg gctgcgccgg gctgtgctct catggtggtc gccatcggcg ggtgcgcaca
    16021 cctgcggacc ggcggattcc tctgcgggac gcccgaggtg gcgctggtcg acatggccgg
    16081 actcgccatg acgaggcagt acggcctgcc gagcatgagc ggcggcatgc tctcggatgc
    16141 tcatgccgcc gacttccagg ccggctcgga gggcgccctt ctggccgcga cctccgtgct
    16201 gtgcggcgcg gacctcctca ccggcgccgg gctggtcaac agcgacatgg ccgtgtccac
    16261 agccaaggcc gtcctcgact gcgacaccat cgggtcggtc aagcggctcc tcgagatgcc
    16321 cgacgccgtg gatgacgcat cgacgctgat cgaggacatc gcagcagtcg gccccggcgg
    16381 tcagttcctc gcgcggcgca gctctcgcga gaactcgcgc cgcggcgaga tctggtgtcc
    16441 gtccgtgttc cagcgcgacg gtctctcagc gcatgcgggg cgttccctgg tgagcgacgc
    16501 gctggaacgc gccgcggcgt tgctggccgc gcatgcgccg acacccattc ccgacgacgc
    16561 ctgccgccag gcccgcgccg cgttcgagcg gtacgtacgc agcgcgggca gccgctgaac
    16621 aaggagcacc ggaagatgga gacgcagtac ccgacccgga tgggtgacgg ctcgctgacc
    16681 cgcttgacgc gcagcgagct gcgcgctgag ctcgaagagg gcaccctggc ggcgtcgagc
    16741 cgggccaagg tgccgccgct gtctgcgggc gagctcgagc acctgctcga catctgggcg
    16801 tcgccggcca agttctccgc cgtcgacatc ggcgacgaag tcgtgctcac ctacgacggc
    16861 tcggggtcca agacggtcgg cacgcgcacc caggacctgc tcatctacga gcagatgttc
    16921 ggagccgact cggtcgagct cgggcacgtc gactactcct tcaaggccat ccggaccatt
    16981 ctgcccttcg aagcccagag catgaaagac gcacaggcgg tcctcacggt cccggtgatg
    17041 tacggggcga tgcccaacct gggcctgtac tcgaagcccg acgggccggc gcccaactgg
    17101 tcggagctgc tgccgctggc acgggtggac gaggcgcggg cggctcagga ggaagcggtc
    17161 ggatacgccg tcgaagatat ggtgaagacc gccgacgcgc tgtgggatgc aggcgcggac
    17221 ggcatcgact tcgacaccgc cggcgcggcc ggcgacggcg acttcctggc caccctcctc
    17281 gcgatcgagc gcatccgtgc ggcccatccc gacatgggca tccaggtcgg catggccggg
    17341 gagttcgtgc tcgggatgca cggtcagctc gagtacgacg gcgtgcgcct ggccggcctc
    17401 tggccgcgcg ggcagatgga gcttgtgcgc cgggcgggcg cgacgatcta cggcccggcg
    17461 atcaccgtca acacgcgccg gacgggggcg tggaacatcg ctcgcgcgct gacgctcgtc
    17521 aagccgtgca tggaggtcgc cgagatcccc gtgcacatga acatcggcat gggagtcggc
    17581 gccgtcccca ccaccagcta cctgccgctg gacgccgtgg cgcgcgcctc tcgggcaacc
    17641 gtcgacatcc tgcggctgga cggcttgtag gtcggtggcg gcgaccctct cgggatgccg
    17701 tgtgcccacg cgaccgcctc ggggatgtgc gggatgcgcg ctgctggaga tctggtggcg
    17761 cgcatgcaga tcactcgggg gatgcggatc ggcgacgcca aggcgtacgt cgcagcgcga
    17821 ctcggggtca gccccgcgga cctctccgac cccatcgcca tggaggacgc gcgaagggag
    17881 tggcagctcg ggcgcatcac caccttcgag atcagctacc ctacccagtc gagcgccatg
    17941 gaggccaagt tcaggatcgc tgaactgctg gacctgccga tcaactgcgt gcgacgcttc
    18001 aggcggcacg cagggcttgc gtgagcccga gcggctggga gggcgtgacg cgcgaatgtg
    18061 gctaccgcgc ctgcgaccac cactttggca catgagcggg agcatcgccg ccctcagggc
    18121 cgatggcctc aggggctcgg tcgagcgcgc aaaggagctg atggcatgac tcgcagagga
    18181 gaagggatct acgcagaagc agccgagagg gccgagatcc gcggccctgc gtcgcgcggc
    18241 cctggaggag ccccatgacg gcgacgatgg accgcgagag actctcggcg ctcatggcgc
    18301 gcgaagacga ggacttcatc gcgcgccacc ccaagtcccg cgagctcttc gaacgcgccc
    18361 aggcgcacct gctcgacggg gtccccatga actggatgac gcgctggtcg agcccgtttc
    18421 cgccgtacgt cgcggaagcg cacggcgccc gcttcacctg cgtcgatggg cacgactaca
    18481 tcgacttctg cctcggggac acgggcgcga tgaccgggca ctccccggag ggatccgtgg
    18541 ccgccgtggc cgctcaggcc gcacgcggca tcacgaccat gctgccgacc gaggactcgc
    18601 tctgggtggc cgaggagctg tcgcgccggt tcggccttcg gtactggcag cttgcgctga
    18661 cggccacaga cgccaaccgc ttcgccgtcc gcctggcccg ccacgtcacg gggcgcccga
    18721 agatcctcgt ctacaactac tgctaccacg gctccgtgga cgagacgatc atcaccatca
    18781 acgaaggcgt gccggggccc aagcccggta acgccggccc gccggtggac ccgaccgtca
    18841 cgagcaaggt catcgagtgg aacgacctcg aggcgctcgc acgggcgctc gagccacggg
    18901 acgtggcctg cgtgctcgcc gagcccgccc tcaccaacat cggcatcgtc ctgccggacg
    18961 agggctatca cgctgagctc cgccggctga cgcgcgagac ggagacgctg ctcgtcatcg
    19021 acgagacgca caccatctgc gccggcccgg gcggctgcac ccgggcctgg gggctcgaac
    19081 cggacgtggt cacgctgggc aaacccatcg gcagcggcgt ccccgccgcc gtctacgggt
    19141 tcgaccagac ggtcgccgac ctcatccacg ccgactggga ctaccacgcc gccgacgagt
    19201 gcggcatcgg cggcaccctg gccggcaaca tgctgagcgt cgcggccgtg cgagcgacgt
    19261 tgggtgaggt gctgacgcag gaggcctacg accgcatgat cccgctcgcc ggccgctggg
    19321 ccgacggcgt gcagagcgtc atcgacgagt tcgccctgcc gtgggtcgtc aagcggctgg
    19381 gatgccgggc ggagtactgg ccgcgacccg tgccgccgcg gaacggcggc gaggcggccg
    19441 cggccgagga cgaacagctc gaccgctacc tgcacctctt catgctcaac cgcggcatcc
    19501 tcatgacgcc gttccacaac atggcgctga tgtcgccgca gaccacggcg gccgatgtgg
    19561 accgtcacac cgaggtcctg cgcgaggctg cccgcacgat caccggttga gagcgtgggc
    19621 tatccccgct cgctcccgcg gcccacgaga tcggcgtcgg cggcggcaac gagccgccgg
    19681 agtccagcgc gcacatcgtc ggcgagcggc tcgggcgcgt gcgtgcgcag gatcccgcgc
    19741 gccttctcct ccgcgttctc cagagcgccc ctcttgccgg cgccgagcca ggcatcgtac
    19801 gacgtccggt cccacacgcc cgggcgccag atctcgccca tgtgcgcccg ggtgtgcttc
    19861 tccgccaggt aggtgccgtt aggcccgacc cggtggatca cgtccagcgc aagcgtctcg
    19921 tcactgacct cgatcccgcc gcccatcctc ttcacgatgc cgtagacttc ggcctccatg
    19981 agcaggtggg cgtagctcag ggtcttggag ccgtcgagca gaccgcagcc gctcatcatc
    20041 tcggcgccgg ccgagacgct ggccaaggtg gagagcgcgt cgtccaccgc ggcttgccag
    20101 cccggctcct tcgcgccgct ggccaaggtg cccatgttca tcgcgaggcc gaagtgatgg
    20161 gccatctgcg tggccgcggc ggccagcagg tagtcctcgg gactcccccc cgtgtagccg
    20221 cccgtcttga ggtccatcac ggacggcgca ccggccagga agacgggagc gccggggtgc
    20281 gccagttgca ggagaacgat tccggcgagc acggccgcga ggttgacgac caggttgccg
    20341 gccatggtcg cgggagcgga tgcacaagcg agcgtcagcg agaggaagcc gacgggcacg
    20401 cccgcctccg cgcacaccag cgccgcctcg aggctgacag cctcattgcc cagcggatcg
    20461 atcgggcagg cgatgaggct catgatcggc cggcggcgga gctcctcgcc accgccggcc
    20521 actgcgcgcg ccatctcgac gacgcggcgc gcgatgtcct cgccgaccac gtcgaccgcc
    20581 tggaagtgct tgctcgtgtt ggagaagacc gcctcggcct cgtgaagcgg gcgcgtcgag
    20641 agcgggacgt cctcggccgt gacgaccggc ccccagtaga agctcacgca agggagggcg
    20701 tcgacgaagc gcgcggagtc ggcgacgtcc tgtctggtgc tcggcctcat ccgaccggtc
    20761 tccgggtcga tcacgcgcac gccacaaccg tcgttgctga gatggcaggc ggagccgtcg
    20821 aggacgatgt cgcagcgagg gtcgcgagcg gcgaggagcg gcgcccgcgg cgccgcatcc
    20881 agcgccgctc gcaccacgtc ctgcggcaga cgcgcgatcc gggatgcgcg gtcgacgggg
    20941 cagccgcccc gctgcagcgc gtcgagtgcg cgctcgagcg ggaaccgcac cccgacctcg
    21001 gccagcgtcc agaggatggc ctgctccagc cgatcgacgt cggcctcgcc gagcacccgc
    21061 cagcgcaggc gcggccggat ggggtcgatc cgcaccgtca ctcctgtccc gaaccttcct
    21121 ggtcgccgcc ggagatgcta ccggccgggt ggcggccgcg tcatcccgcc ggtcggcctc
    21181 cgccgggcct cgccgagcca gctgccggac cgtcgcctgc cggccgccag cccggggcga
    21241 ggcaaccggg ctccgcacgt agatggggcg gggaggcgcc tcacacctcc ccgccccctc
    21301 ggtcgtagct accgcccggc gatgccctag agcttctcgt cgcccggagg cagaggcgtc
    21361 tctccgctag gacccggcgc gatgggaatg tcgggaatcg ccgtgtccct gacgttgtcc
    21421 tcgtcgagcg ccgcacccgc tgcgccctgc ggcggaccct cgacggcgtc cggggagggc
    21481 agctcctggt cgacctcgag gatgcccagc tccttggcgt gctgcggctc cttctccggc
    21541 aggatgtgcc gcaggaagcg cttggtgcgg tcgtcctggg ggtggaagaa gacctcgctc
    21601 ggaggaccct gctcgatgaa gtagccgtcg tccatgtaca cgacgcggtc ggccacgtcg
    21661 cgactgaagc ccatctcgtg gctgaccacg atcatggtca tgccgccctc ggcgagttcc
    21721 ctcatgacct tgagcacctc gccgatcagc tcggggtcga gggcgctggt cggctcgtcg
    21781 aagagcatga gcaccgggtc catcgccatg gcacgggcga tggcgacgcg ttgcttctgc
    21841 cctccggaga gttggctcgg gaagtagtcc atgcgctcgc cgagccccac cttggtgagc
    21901 agctcgtggg ccttcctgac ggcttcctcc ttcttcacgc ccttcacgag gatcgggccc
    21961 tcggtgacgt tctcggtggc gtccttgtgc gggaagaggt tgaactcctg gaagaccatg
    22021 ccgcacttca tgcggatctc gtgtacgtcc ttcttgaagc cgctgccgtg gctgccgcag
    22081 gtgacgtgca cgtccccgac cctgatctcg ccgctcgtgg ggtcctcgag gaagttcagg
    22141 cagcgcagga aggtgctctt gccggagccg gacggcccga tgagcacgac cacctcgccc
    22201 ttgtagacct cgaggtcgat ccccttgagc acgtggttct tgccgaagta cttctgcagg
    22261 ccctcgacct tgacgacgag ctcgcgtccc ttgtcgtcgg tgatcgtctc atgggtgtat
    22321 gtctttgttc ccatgctact tcgccttccc cttctctttc tcgcgggcct tgtcgcgcgc
    22381 catgcgcgtc tcgagcttgg cctgccagaa gctgaagaag atcgtcagcg cccagtagaa
    22441 ggccgccgcc acgagcaggg tggagaagtt gttgagcgag gaggcgccgg ccagctgggc
    22501 gcggcgcagc agttcctcga cggtgatgac cgagaccagc gacgtgtcct tgatgagggc
    22561 gatgaagtcg ttgcccacgg ctgggatgac gatcttgaac gcctgcggca ggacgatgcg
    22621 ccgctgtgtc tgccagccgc tcaggccgat ggcccaagcg gcttcgttct ggcccttggg
    22681 caccgcctgg atgccggcgc ggaacacctc ggtgaggtag gcgccgtagt tgagcgagag
    22741 ggcggcgaca cccgaccaga acgccggcgg attgtaggtg tcaggcagcc cgaacgccct
    22801 gatgaactcg gggacgccct ggtagatcac gatgatctgg agcagcagtg gcgtgccgcg
    22861 gaagagagag gtgtaaaagg tggcgagcca gtacgggatg cggccgatga ccatcctgaa
    22921 catgtactcg cggctctggt agcggtccca ggcctgcttg aaggtcatct tcttggagag
    22981 ccggccgagc gagccgaaga gggcgaagac gcaggcgagc aggatggaga tgatcgtcag
    23041 ctcgacggtg acgatggcgc ctgcggcgat gtaccagaac cactcctgca tgacctcgaa
    23101 gttgatcagg ctgccgcccg aggcgccgcc ctcggacgcg aactcccagg cgtagacgtc
    23161 ggaggtctcg tcggtcctct cgtcgtacac ctggagttgg gtgccggcgg tgtcgaacag
    23221 cgtgacctgg aactggaagt cgccctgcgc gaacgtaggc tccgtccact tcatctgcag
    23281 cgtcgggttg cccggcgaca gctcgatcat gctcgccggc tcggagagca cgatctcctc
    23341 gccatcgacg gtcaggaccg acgccgcggc gtcgagatcg gtgccgttga ggtccaggtt
    23401 gatgttgatc tggtctccgg accacttcgt ccgagtgccg ctcgggtcgg ggatgacccc
    23461 gagtacgatg agcgtcccct ctggcgccgc ggccgcctcc tccccgggcg acgggctggc
    23521 cccgtccgtc gccagggcca ctggcgtgag cgcggtgaag aagagcgcga gcagggcggc
    23581 gaggaggccg acgctcacga agcctgcgcg tctgtgcatg tctggcgaac ctttcctcga
    23641 aatgacagcg gcgggggcgc cgaacaagcc gcccccgccg cgtcgaatca ggcggcgggt
    23701 cccttaggac tcaccgtcag gccgtcaccc tattccttca ccgtcaggtc gatgccgttg
    23761 taccactgct tcgacatctc ggtgagcgag ccgttctcgt gcatctgctg aacggagtag
    23821 gtcagcaggg cgacccagtc ggcctcgccc ttcttgaccg ccatcgcgag gtcctcgtag
    23881 tagagcggct tgcccgagaa ctccatggcc ttgccctcga ggatggcctg ctggccggtg
    23941 gggccggcgg tcatcacgaa gtcgaggttg ccgttgagga ggtccgggaa ggcgtccacg
    24001 tcggtggtgt acgtcttgac gtccgcttcc gactccttct tgaggaagtc gtaataggtg
    24061 gtctgggcgc cgacgccgac cttcataccg gcgaggtcct cgacgcctgc gatctgcgtg
    24121 ccgcccttct tcacgaagac ctgacccgag gtgtagtagt acgggtcgct gaagtccagg
    24181 gtctcctgac gctcgggcgt gatcgtcatc gagccgatgg agacatcgta gcgaccgttg
    24241 ttcaggccgc ccgggatggt ctcccatgcg ggatgctccc acttgatggt gaggccgagg
    24301 atctcggcca tgcccttggc gacgtcgacg tcgaagccga tgatctcgtt ggtgtcgggg
    24361 tcgaggtagc tctgcggtgc gtagttggag tcgttggcga ccacgacctc gcccttggtg
    24421 acgatgtcca aggccagccc ggtcggctct ttgccgagga tggccgtagc ccgctccatg
    24481 gccgagcctt cgatcgccgg cgcttcggtc gcggtcgcgg tctcctcggt cgctggttcg
    24541 tcttctccgc acgcggcgag tacgcccgcg agcgcgatga ccgcgacgag cagcgcgatc
    24601 accaggatgt aggtcttgcc ctttctcatg cctccgtcct ttccggctga cttttcccga
    24661 cgccgccgac ccacgggtgt ggatgcgcgg atggcggccc ccaccgcctg tgtcaggacg
    24721 ttgcgcgcca cactccccca gcgcgacgtg tcgagttgga tttatacagc accgagccca
    24781 aaagacaacc ctcgcgaggc tgacggcgta ccgtcttgac ccgagttgtg agtatctgtg
    24841 cgctgggcgg cgcgtcagcc tatgccggcc agtcgcctga gttcctcgtc gactgcggga
    24901 tcgagggcgg ggggacggtg ctcggcgacc gccgccgcga cgcgctcgtg cgcgcgatcg
    24961 atggcggtct cccgcccgcc ggcggtccag tgccggtggg tcttgtccac ggcgagggac
    25021 gggccggcgg cgggcggagc ctgtccgccg gccgagccgc cggccgccgt caggtccact
    25081 ccggcggcga tcgtgcgtgc gtgcgtgagg gtgtcgaccc cgagcgccat ctcctcgtag
    25141 ctgaagatcc ggccgccgga gaccatgccg accccggtga gcatgtcgac gccggcggcc
    25201 agcgacagat acgtcgtcgt ggtgtcgtcc agcgccgact gccagccggg ctccttggcg
    25261 ccgctcgagt tcacgccgca ttgagtgggc agcccgtaga agtggccaac gtcgccgacg
    25321 gccgccgcca tgatggtgtc ctccggcgcg ccgccggtga agtcgccgct cttgaggtcc
    25381 atcacgctcg ggatgaagca gatgaacacc ggcgcgccgg gacagacggc ctggatcgcg
    25441 cacaccgagc tgagcgcctc ggcgacgccc accacgagcg agccggccat ggtgatcggc
    25501 gtcgtcgaac cccccatcgg catcgtcacg aagccgatgg ggacaccggc ctcggcgaag
    25561 accaggccgg cctcgagggt gccctcgtcg ttacccaggg gcgtgacggt gccgagcagg
    25621 gcgctgatcg gcggctcggc gcggagtctc tcctccgagc cactcaccgt cagcgccatc
    25681 tcgaccgcca ccccggcgag ctccggctcg acgacggtga cggtctgcac gtgcttgccg
    25741 gtgttggcga cggcgaggta cagctcgtgc aggcctcgcg tctccacggg ccggtcctgg
    25801 gcgctgacgg ccggccagca gaagtcgatc tcgggcagcg cgtcgacgac acggctgatc
    25861 gtcgccacgt cgtcggccgt agtgcctcgc ttctcgcctg tctcgaggtc gtagatctcc
    25921 acgcaacagc cgtctgtggt gacgagagac cgttcgccgg tgatgagggc tgcgccggcg
    25981 cgggcgccga gggtcatgtg cgccggcgcc acgtccacca gccggcgcac aagctccggg
    26041 gggaaggtga tgcgcgtgcc gtcggccgtg gccccctgcg ccacgagggc agcacgcgcc
    26101 cgctccgaag ggatcgccac gccggtctga gcgagcacgg cgagggcggc ggcgtccagc
    26161 ctttccaggt cgtcctggga caggatggcc agacgaccgg ccgatccgat ggtgtgcatg
    26221 aggttctccg tcatcgtcac gttctccact tcgcgatcga cagcgtccgc ggccggagca
    26281 gggcggcaag gtcagcgctg cggatcttga gcatcgtgct cgtcgtgccg ccgccgcagt
    26341 acacgacctc cggcacgaag acgccgggat cggcgatcac cggtacctgc gtggacaggg
    26401 cgcagggcga gaccgctccc ggggcgtagc ccgtgtgctc ccgcacctct tgacctcggg
    26461 ccagacgtac ctgggcggcc ccggactcgc gtgccagccc gtccgcgtcc accgtcgcgt
    26521 cgccggtcac cagagccagc accggccgtt cgtcgagcat gaagagcaag gacttgacca
    26581 cgtccccgac gtcgacgccg agcgcctcgg cggcgagctg ggccgtcttc gaggacgacg
    26641 gcaggtggag gatctcgtgg gggatgttct tcgcggccag ctcgccgtgg acgtcgtcgc
    26701 cggatcgcac gcggcgtgcc tcctcgtcgg gggcgccagt ctagcacgtt gtgacgccgc
    26761 tccagacgcg ggtaccatcg gtcaatggac cagtcccatc gcgatgaggt gcccgcgccg
    26821 tctgacgccg atacgccccc gcgggagctg ccggctcgca gcgtgcggtc ccgccggctg
    26881 cgccttctcg gcgacctgct gctgatcgcc ggaggcctcg tgctggccta cccgttctgg
    26941 tccgcggcct acgcccaggt gcagcagtcg cggctcgaca ccacgtaccg cgcccagacg
    27001 gcggcgttcg tcgaggacat ccacgccaac gtcgacacga agccacgggg agtcacggac
    27061 gaggagctgg tccgccggct cgccgtgcgc taccggcgcg ggctcaaggc cggcgacccg
    27121 atcggccggc tgcgcatccc gcgcatcgac ctcgaccgcc tcgtgcagca aggcgtcggc
    27181 ggccggctgg cgcttgaccc ggacggtgac cgctcgctgc tgcgcagcgg tccggtccac
    27241 tacgcagtca ctccgcttcc cggcctcggc gaaccgttcg cggtcgcggg ccatcggacg
    27301 acctacggcg cgccgttctt caagctcgac aggctacgca agggcgatcg catcttcgtg
    27361 gacacgccct acgggaagtt ccgctacgcg gtagccaaga cgacgaccgt ggacccctcg
    27421 gacatcggtg tgctccgcga ccgcggctac ggactcgtgc tcaccacctg cacgccgccg
    27481 tacagcgccg agcaccgcct catcgtgtgg gcgaagctgg agagggcgcg gccgccgcgc
    27541 gcggcgcgcg accgctgacg gcgcttggtc tcgatgtccg cgcggatcag cggacggcgg
    27601 gagcgaccgg gtacgccggg cgctccgagg cactgccgga gagcagcatc accagcagct
    27661 cttcccccac ctcgccgccc ggatactggg gcggcacgaa gaagagccct tcggcgccgg
    27721 ccatggagct caggatgccc gacccctgcg gacctgtggt cgtgaagcgc agggcgccgc
    27781 cctcgtgggt gaggcggcag cggcgcgcct cggtgcggct cttggtccgc tgcaccggct
    27841 ccgccgacgc ggcgtagaac cagggccggt agatgtccgg ccggccctgc agcgccagga
    27901 tggccggccg cacgtacatc tcgaagctga ccatggcggc gacggggttc ccggggacgc
    27961 cgaacacaag ggtgccgttc ctcgttccga aggcgagagg ctttcccggc ttggtggcga
    28021 cgccccagaa ccggcgctcg accccgagtt cgtcctgcac ctgcttgacg aagtcgtaat
    28081 ctccgaccga gaccccgccc gacgtgacga ccacgtcgtt ctccagcgcg gccgcgagca
    28141 ggcggcgcgt ctcgtcgggg tcatcgcggg cgatgccgag gaggatgggg tccgcgctcg
    28201 cggccacggc ctggccgtag gcggtgaagg agttggagtt gcgtatctgg cccggggcca
    28261 gctgccggcc ggcacccacg agctcactgc ccgtggcgag gatggcgacg cgcggacgac
    28321 gggccacgcg tacacgctcg atgccaaccg cagcgagcag cccgatctcg gccgcgccca
    28381 ggcgcgagcc gcgctcgaag aggacgtccc cggcgctgac gtcctcgccc tcgcggcgca
    28441 cgttcgcccc cgagggaggg gcggtctcga cggtgacgag gtcgtcgtcc accgaagtgt
    28501 gctcgacctg cacgaccgtg tcggcgcccg gcggcagcgg ggcccctgtc atgatgcgcg
    28561 cggcgttgcc gggcgcgacc ggctccgcca cgtagcgtcc ggcggggata tcgacgacca
    28621 cctggaactc gcgccggcca gcctcgagct ccgccccgga cacggcgaag ccgtccatgg
    28681 cggagttggc gaaggtcggg acctgctcgg gcgagacgat gcgctcagcg gcggcgcgcc
    28741 cgagcgcctg cagcagaggg acctcctcgg acggcagcgg gcggactgcg gccagcacgg
    28801 cgtcgagg
//
LOCUS       DASUMX010000322        12332 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_557736, whole genome shotgun sequence.
ACCESSION   DASUMX010000322 DASUMX010000000
VERSION     DASUMX010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 12332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12332
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557736"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..570)
                     /locus_tag="VFZ86_15330"
     CDS             complement(58..570)
                     /locus_tag="VFZ86_15330"
                     /inference="COORDINATES: protein motif:HMM:NF018590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HEX5643684.1"
                     /translation="MSEMMVLGFENEMEADRFGLKLAELQKDMIVQLGDAAEVVRDPD
                     GKPHVKHGDHIVGAGAMGGAFWGMLFGLLFFMPFLGLAIGAGMGALFGKMGKTGIQQK
                     VLEQMGDAIPPGKAGWFLIIEQMTEDKFLAAVEGTNAKLVRSNLTAEQEEQLKHAFGA
                     KHHKKDEATA"
     gene            complement(638..1387)
                     /locus_tag="VFZ86_15335"
     CDS             complement(638..1387)
                     /locus_tag="VFZ86_15335"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5643685.1"
                     /translation="MADEDAQHPRRGRDATTAAILDAADVLFRERGYEDVTVRDIAEQ
                     AGVSHALVHQYVGSKEDLFRAVLSRNDGYMTQAAPADIGLLESARLILRSRLEPQARD
                     YGRLLMRTALSGVPYDQTTGRFAAVEQLIELAERQAASASASERAEKDLAPRLVGACI
                     GSLYLGWVSGESWLRPAWGLEDMEDDEVASGLERVILSILKDHVPGVASEAASAADVR
                     ELKIELAEMHAEITALRELLKARQQSAEESA"
     gene            1504..3165
                     /locus_tag="VFZ86_15340"
     CDS             1504..3165
                     /locus_tag="VFZ86_15340"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEX5643686.1"
                     /translation="MKSPISLPRGRDIKNDLIAGLTTGLVQIPDAMASAILAAVKPVN
                     GLNTLVIGTPIGALFAGSVFMTVTTTGAIALAVADAVGDAGADERTGVLIMLTLMVGV
                     IQLALGLFKLGWITKFVSNSVMVGFITGVCILIILGQLGDLTGYSSEYSNKVVKTVDL
                     VLNFDEVSWTTLGVGLATVGLILAFGRTPARKFAMVLAFAVVTVGVVVLGLGVQLVSD
                     VAAIPSGLPEFQVPDFSKAAELIVPAIAIAAIGLIQGAGVSKSVPNPGGEYGDDSRDF
                     AAQGAANAAVSFFQGMPIGGSVSASALNVSSGARSRWANVYSGVIVAVVLVLFGSAIE
                     KVPMATVAALLIVAAVGSLKPAAILEVWRTNWAARAIMLTTLALTLLLPIQYAVLLGV
                     AVSTIQYISSASLDVKVRRLVREGEELFREEDPPERVPDREVTILDIYGSIFYAAADV
                     IAGKLPDVRDAKGAVLVLRLRGRGQVGSSAIGLFRRYATDLQQGGGALLLAGVGHDMA
                     DQLKRTGIEDLLGQHSVFPARSLVYRSTEAAYEEGRRRLASETPA"
     gene            complement(3180..3255)
                     /locus_tag="VFZ86_15345"
     tRNA            complement(3180..3255)
                     /locus_tag="VFZ86_15345"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3219..3221),aa:Glu,seq:ctc)
     gene            3384..4352
                     /locus_tag="VFZ86_15350"
     CDS             3384..4352
                     /locus_tag="VFZ86_15350"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5643687.1"
                     /translation="MSEAGPTAEVTFRRAEEADLPRVFAVFRAALNAYLVPAGQEGIP
                     EDEVQSPVYRHFLEHDGERFWVAEAVVEDLTGGGSGTLGAGGRQIVAWGSGLLRGDWW
                     FLSSLFVLPEAQSRGVGGRLYELTATGAPPGAVRATVTDSLQPISNTLYARRGLLPRE
                     VLVGFGGQPREGLAAPRLGTLMPEPLTAAAIPELREIDAAAGGLDRSVDHAFYLSRGG
                     RCGFLFRRRGRPMAYAMLRQDGWVGPVASVRAGDVENVTAYAIAVMAGRDGIDKIRAG
                     VTGRCEGAQRAFWQAGLVFDGTPGLLLSSRPFGRLDRYVVASYGMF"
     gene            4449..4760
                     /locus_tag="VFZ86_15355"
     CDS             4449..4760
                     /locus_tag="VFZ86_15355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643688.1"
                     /translation="MSHADEVITDVRRRRVMARAHEDSFTIAWDYPGRTLLEVRILRS
                     SARPAASADDGPGQGQELVYQGTTGSYRDAGPTPDQSCFYTVFARHPGEEWVRWEEYA
                     R"
     gene            4757..5794
                     /locus_tag="VFZ86_15360"
     CDS             4757..5794
                     /locus_tag="VFZ86_15360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643689.1"
                     /translation="MSRRLGLALCLFLALAGLIALPVAAGASGDGDKSGGDKASALES
                     GAAAAAAAAQADPSIAAVLAGTDAAIEQTLPWGGTEGEPAGYRIEYRWPAAQAAAVDQ
                     EWPLLRSDASAPQPPYDSTMYRVRASEVTGLQVDVRAEGSQVIQVMPQNGETEYVLEE
                     QTWAPFSWVPWFTADPWVIAPVFIALAILLMVRAWLRSRAWNRRLPSMTRHDRQFIAR
                     VAVLLFLLAGFAWQFYEGWFAATGPSLGSSSAGSLAALPLLLIPPGLFVAGLILELSH
                     APHRGSWALLAVLCGAASVYFLATAMTGSTTNLNLTYYILLAVLGLISIPRAFSSGKM
                     GWSRMAAPRYG"
     gene            complement(5823..6197)
                     /locus_tag="VFZ86_15365"
     CDS             complement(5823..6197)
                     /locus_tag="VFZ86_15365"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5643690.1"
                     /translation="MKFVHVATRTRDLEGAIKFYEQLGLHESRRSELTKGKATLVFME
                     PEEGNFAIELVYNWGKDDAYPGGERFGHFAFETDDIDAVLPAIERCGGRVTRQPYLLE
                     GDGPRLAFIEDPDGNAVELIQR"
     gene            6235..6786
                     /locus_tag="VFZ86_15370"
     CDS             6235..6786
                     /locus_tag="VFZ86_15370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019071023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEX5643691.1"
                     /translation="MSISAFGDKVPQFGPGVFVHVDATVIGDVVLEEGVTVWPGAVLR
                     GDVEQIVVGAHTSFQDGAIAHSDPGFPVRIGAACIIGHGAIVHGATIATGCLIGMHAT
                     LLNGCEIGEQSIVAAQALVPEGRSCPPRSLFIGAPARVIRKLSDEDLAPLTGVHERYS
                     ARGRAYTEQGLGADLSAFRGPAD"
     gene            6816..7400
                     /locus_tag="VFZ86_15375"
     CDS             6816..7400
                     /locus_tag="VFZ86_15375"
                     /inference="COORDINATES: protein motif:HMM:NF024769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HEX5643692.1"
                     /translation="MAVIAEADRFFAVDRAAWREWLAAHHEERTQVWLILHKKHVEEP
                     SVRYEEAVEEALCWGWIDGLTNSWDERSYAVRFTPRKPGSVWSESNVERVTRMIAEGR
                     MTPAGQALVDEAKRRGTWDEAASGRLDVTPPDLEAALAGVPAAAARWNEWPATPRRMY
                     VYWVLDAKRPETRARRIADVVRRAAAGLRPGEKG"
     gene            complement(7415..8161)
                     /locus_tag="VFZ86_15380"
     CDS             complement(7415..8161)
                     /locus_tag="VFZ86_15380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012350229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HEX5643693.1"
                     /translation="MQTAARALARSARLAVFTGAGVSKESGIPTFREPETGLWARYDP
                     MQLATPEAYRRDPAFVWSWYEHRFGVAAEAEPNPGHRAIAELEGLLPKVVVITQNIDG
                     LHQRAGSSHVIELHGTMHSFRCIDGRHRGYRREDFADQGEKPPRCPECGDYLRPEVVW
                     FGEGLPPGAISAAQRLGAACDVMLIIGTSGVVYPAAAIPMIAKEAGATLIDVNPEPDA
                     LTRTSDVYLRGPGGEVLPELVRAVREHLGA"
     gene            8253..8807
                     /locus_tag="VFZ86_15385"
     CDS             8253..8807
                     /locus_tag="VFZ86_15385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643694.1"
                     /translation="MSEEPQRTEGPGPAPGPEAEEPAGGAGPAVGAQGGGGAHAGLRL
                     LAAAVMLPWLLAYGIFGVSAVTRSAQAYGAGLERIDPGYTRFVSPGELAYVGALLLAA
                     FAVLLACSLLLLFRHRTATGWLPVLLVAAGLTAGSVWAAVQGGLHPILWVLFFFGLAC
                     CTAIALARVLQVTRSGRRGRIASP"
     gene            8804..9157
                     /locus_tag="VFZ86_15390"
     CDS             8804..9157
                     /locus_tag="VFZ86_15390"
                     /inference="COORDINATES: protein motif:HMM:NF024388.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3870 domain-containing protein"
                     /protein_id="HEX5643695.1"
                     /translation="MSTSIFVGHAKPPSNTVSGQMYTILSVVCEVDMETGVIVAAEFT
                     VATELAKDYLNRLLAGRNLTTDEDAIVAELEGCYFSGTQKALIQCFRDMAKRYRAQAG
                     PSRPDAPAEPAPRAG"
     gene            9254..10396
                     /locus_tag="VFZ86_15395"
     CDS             9254..10396
                     /locus_tag="VFZ86_15395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008618811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HEX5643696.1"
                     /translation="MVAVDGITSFEPPRRVLMGPGPSDVHQRVLDAMARPTIGHLDPE
                     FVRMMDELKDLLRYAFQTGNRVTFPVSGPGSVGMETCFVNLVEPGDKVLVCRNGAFGG
                     RQLENVERTGGVPVVVDDDWGTPVSLDKLEWAFDAHPDVRVVSFVHAETSTGVRSDAQ
                     AIAAIAHAHGALVIADTVTSLGGIPVLVDEWGLDAVYSGSQKCLSCTPGLSPVTFGEA
                     ALEKVKARETKCQSWFMDLNLVLPYWEGGARTYHHTAPINPLYGLHESLLMLREEGLE
                     AAWRRHAELHAALVEGLEGLGLSMFVEAPYRLPQLNLVSVPEGVDDAWVRAEALRRFG
                     LELGNGLGVLAGKVWRVGLMGTSCTQANVDLCLDALGTLIPEAAAK"
     gene            10546..12204
                     /locus_tag="VFZ86_15400"
     CDS             10546..12204
                     /locus_tag="VFZ86_15400"
                     /inference="COORDINATES: protein motif:HMM:NF015882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HEX5643697.1"
                     /translation="MTDETSKGPEPTVEELKAKAYQPMEDAAVLHPFYRGKIETTLKC
                     CIRDFQDFGIWYTPGVSKPCLDIKDDPEKVYEFTNKWNTVAVVSDGTRVLGMGDIGPK
                     AGMPVMEGKSLLYKYLGGVDGFPLMVDTKDPDELIKFVKLVQPGLGGVNLEDIAQPKC
                     FRILDTLRAECEIPVWHDDQQGTACVTLAGLINALKVVGRRIDDVEIAFIGSGAANVA
                     ISRLIFAYGADPAKCRIVDSKGILHKGRADIEAVKDEWVDKWKYCQITNAEQRQGTIA
                     DALRGAHVCIALSKQGPDTIDKEWIRGMAHDPVVFLCANPIPEMWPWDAKEAGAAVVA
                     TGRSDFPNQVNNSVGFPGIFRGTLDVRARTITDEMCIAAAEELAKVAEDKGLSADYIM
                     PTMDDWEVFPREAAAVAMKAIEQGIALRTDLTFEQEVAEATAIIARARGMVQDQMAIG
                     YIPMPEGSRAPAPTKAAARAVARGAGDYAGEMADKAVDRIGPAADKIAAATAAAIDKA
                     TDAIKRAMKKDDGVTDAEVVSEEPPEWKPPAAEPEPPADAEKTE"
BASE COUNT         1768 a         4380 c         4318 g         1866 t
ORIGIN      
        1 ggcgggcggc cctgtcggcc tcccgcccgc gatcacgcgt tcagcaggcg acgccgctca
       61 ggcggtcgcc tcgtccttct tgtggtgctt ggcgccgaag gcgtgcttga gctgctcctc
      121 ctgctcggcc gtgaggttgg agcgcaccag cttggcgttg gtgccctcga cggcggccag
      181 gaacttgtcc tcggtcatct gctcgatgat gaggaaccag ccggccttgc ccggcgggat
      241 ggcgtcgccc atctgctcca gcaccttctg ctggatgccg gtcttgccca tcttgccgaa
      301 gagggcgccc atgccggcgc cgatggccag gccgaggaag ggcatgaaga agagcaggcc
      361 gaagagcatg ccccagaagg cgccgcccat ggcgccggcg ccgacgatgt ggtcgccgtg
      421 cttgacgtgc ggcttgccgt cggggtcgcg caccacctcg gcggcgtcgc cgagctgcac
      481 gatcatgtcc ttctgcagct ccgcgagctt gaggccgaag cggtcggcct ccatctcgtt
      541 ctcgaaaccg agcaccatca tctcgctcat agaccctcct gcagtcgtag cgcgctgatg
      601 cagcgaactt accaacggtc agagcgcgat acaagcatca tgccgactcc tccgcgctct
      661 gctggcgggc cttgaggagc tcgcgcagag cggtgatctc tgcatgcatc tccgccagtt
      721 cgatcttgag ctcgcggacg tctgcggccg atgctgcttc cgaggcgacc ccgggcacat
      781 ggtccttcag gatgctgagg atcacgcgct cgagtccgga cgccacctcg tcatcctcca
      841 tgtcctcgag accccaggcg ggccgcagcc aggactcacc cgagacccag ccgaggtaga
      901 ggctcccgat acatgctccg acgaggcgcg gggcaaggtc cttctcggcg cgttcggagg
      961 cagacgcgga cgccgcctgc cgctccgcga gctcgatcag ctgctcgacc gccgcgaatc
     1021 ggccagtagt ctggtcgtag ggaacgccgc tgagcgccgt tcgcatgagg aggcgcccgt
     1081 agtcgcgggc ctgcggctcg agacggcttc tcaggatgag cctcgcactc tcgagcagac
     1141 cgatgtcggc gggcgccgcc tgcgtcatgt agccgtcatt gcgcgacagc acggcgcgga
     1201 agaggtcctc cttgctgccc acatactgat gcaccagcgc gtgcgagacg ccggcctgct
     1261 ccgcgatgtc gcgcacggtg acgtcctcgt agccgcgctc gcggaagagc acatccgcgg
     1321 cgtccaggat ggccgccgtc gtcgcgtcgc gcccgcgtcg ggggtgttga gcgtcctcgt
     1381 ctgccactac ggcctcccac atctgctgtc gggatggacc cgtgcgatgc ccctgcgcgc
     1441 cgtgtcccac cgtatcttac ctctggtaat atgcgccgta tacgagtcat cgggagccac
     1501 cacgtgaagt cgcccatcag cctgccgagg ggcagggaca tcaagaacga tctcatcgcc
     1561 ggcctgacca cgggcctcgt gcagatcccc gacgccatgg cctcagcgat cctcgccgcc
     1621 gtcaagcccg tcaacggact caacacgctg gtcatcggca cgcccatcgg cgctttgttc
     1681 gccggctcgg tcttcatgac cgtcaccacg acgggcgcca tcgccctcgc cgtggcggac
     1741 gccgtgggtg acgcgggagc ggacgagcga acgggcgtgc tcatcatgct caccctcatg
     1801 gtcggagtca tccagctggc gctcggcctc ttcaagcttg gctggatcac caagttcgtc
     1861 tccaacagcg tgatggtcgg gttcatcacc ggcgtctgca tcctcatcat cctgggccag
     1921 ctcggcgacc tgaccggcta cagcagcgag tactccaaca aggtggtcaa gacggtggac
     1981 ctcgttctga acttcgacga ggtctcgtgg acgacgctgg gcgtgggcct ggcgaccgtg
     2041 gggttgatcc tggcgttcgg gcggacgccg gcgcgcaagt tcgccatggt gctggcgttc
     2101 gccgtcgtga ccgtcggagt agtcgtgctc gggctcggcg ttcagctggt cagcgacgtc
     2161 gccgccatcc cgtcggggct gccggagttc caggtccccg acttctccaa agccgcggag
     2221 ctcatcgtcc cggcgatcgc gatcgccgcg atcggcctga tccagggcgc cggggtcagc
     2281 aagagcgtgc ccaaccccgg cggcgagtac ggtgacgact cacgcgactt cgccgctcag
     2341 ggcgccgcca acgccgccgt cagcttcttc cagggcatgc ccatcggggg gtccgtctcc
     2401 gcctcggctc tgaacgtcag ctccggcgcc cgctcccgat gggcgaacgt gtactcgggc
     2461 gtgatcgtcg ccgtcgtcct ggtgctcttc ggcagcgcca tcgagaaggt gccgatggcg
     2521 accgtggcgg cgctgctcat cgttgccgcg gtggggtcgc tcaagccggc cgccatcctt
     2581 gaggtgtggc ggacgaactg ggccgcgcgg gcgatcatgc tgaccaccct ggcgctcaca
     2641 ctcctgctgc ccatccagta cgcggtgctg ctgggcgtcg ctgtctccac catccagtac
     2701 atctcgagcg cgtcgctcga cgtcaaggtc cgccggctgg tccgcgaagg ggaagagctt
     2761 ttccgcgagg aggatccgcc cgagcgtgta ccagaccgcg aggtcaccat cctcgacatc
     2821 tacgggagca tcttctacgc ggcggccgat gtgatcgccg gcaagctgcc ggacgtgcgc
     2881 gatgccaagg gggcggtcct ggtgctgcgt ctgcgaggcc gcggccaggt cgggagcagc
     2941 gcgatcggcc tcttccggcg ctacgcgacc gacctgcagc agggcggcgg agcgctcctc
     3001 ctcgccggcg tcggccacga catggccgac cagctgaagc gcacgggcat cgaggacctg
     3061 ctggggcagc acagcgtctt cccggctcgc agcctggtgt accggtcgac cgaggcggcc
     3121 tacgaggagg gccgccgccg gcttgcttcg gagaccccgg cctgaggaag ggcggctggt
     3181 ggtagcgcat acgggattcg aacccgtgtt accgccgtga gagggcggcg tcctaggccc
     3241 ctagacgaat gcgccacgca agaacggggc tgctgcggcg gcgggcgcga gcccacagcg
     3301 gcagcggcgg ataagggtag cacacgccgc gcgaacggaa aagccgtcga ctgccgcgcg
     3361 gtcccccgag taggctagcg cccgtgagcg aggccgggcc gacagccgag gtgacgttcc
     3421 gccgggcgga agaggcggac ctgccgcgcg tgttcgcggt gttccgcgcg gcgctcaacg
     3481 cctacctggt cccggccggc caggagggca tccccgagga cgaggtacag tcgccggtct
     3541 accggcactt cctggagcac gacggcgagc gcttctgggt ggccgaggca gtggtcgagg
     3601 acctgaccgg cggcgggagc ggcacgctcg gggccggcgg ccgccagatc gtcgcctggg
     3661 ggagcgggct gctgaggggg gactggtggt tcctcagcag cctcttcgtc ctgccggagg
     3721 cgcagagccg cggtgtcggt ggccgcctct acgagctgac ggccacgggc gcgccgccgg
     3781 gagcggtgcg cgccaccgtc actgactcgc tgcagcccat ctcgaacacg ctgtacgcgc
     3841 ggcgcggcct gctgccccgc gaggtgctcg tcgggttcgg cgggcagccg agggaggggc
     3901 tcgccgcccc gcggctgggc acgctgatgc cggagcctct caccgccgcc gcgatccccg
     3961 aactgcgtga gatcgacgcg gcggccggcg ggctcgaccg gagcgtcgac cacgcgttct
     4021 acctgagccg cggcggccgg tgcggctttc tgttccgccg ccgcggccgc ccgatggcct
     4081 acgccatgct gcggcaggac ggctgggtcg ggccggtcgc gtccgtgcgc gccggcgacg
     4141 tcgagaacgt gacggcctac gccatcgccg tcatggccgg acgcgacggc atcgacaaga
     4201 tccgcgccgg cgtcaccggc cgctgcgagg gcgcgcagcg cgccttctgg caagccgggc
     4261 tggtcttcga cggcacaccc ggcctgcttc tctcctcgcg cccgttcggg cgcctcgacc
     4321 gctacgtcgt cgccagctac gggatgttct gagcgggacg ccgcgcccgc ctccggcagc
     4381 cctcgctccc gtcatccgga cgccccgtga tacggttcgc tcacctgagg agccacttcg
     4441 ggaggcacgt gagtcacgcc gacgaggtca tcaccgacgt ccgcaggcgc cgcgtgatgg
     4501 cgcgcgccca cgaggacagc ttcacgatcg cctgggacta ccccggacgc acgctgctcg
     4561 aggtccgcat cctgcgctcg tccgcgcgcc cggccgcgtc ggccgacgac ggtcccggtc
     4621 agggccagga gctcgtctac cagggcacga ccgggtcgta ccgcgatgcc ggcccgacgc
     4681 cggaccagtc gtgcttctac accgtcttcg cgcgtcatcc cggcgaggag tgggtgcggt
     4741 gggaggagta cgcacgatga gccgtcggct cggtctcgcc ctctgcctct tcctggcact
     4801 cgccggcctg atcgccctgc ccgtcgccgc gggcgcctca ggcgacggcg acaagtccgg
     4861 cggcgacaag gcctccgcgc tcgaatcagg cgctgcggcc gccgctgccg ccgcgcaggc
     4921 ggacccgagc atcgccgccg ttctcgcggg tacggacgcg gcgatcgagc agacgcttcc
     4981 gtggggcggc acggagggag agccggccgg ctaccggatc gagtaccgct ggcccgccgc
     5041 ccaggcggca gcggtcgacc aggaatggcc gctgctccgc tcggacgcgt ccgctccgca
     5101 gcctccgtac gacagcacga tgtatcgtgt ccgggcgagt gaggtgaccg gcctgcaggt
     5161 cgatgtccgc gccgaagggt cccaggtcat ccaggtcatg ccgcagaacg gcgagaccga
     5221 gtacgtgctc gaggagcaga cctgggcgcc cttcagctgg gtcccgtggt tcaccgccga
     5281 tccgtgggtc atcgccccgg tcttcatcgc gctggcgatc ctgctgatgg tgcgcgcctg
     5341 gttgcgctcc cgtgcctgga accggcgcct cccgtccatg acccggcacg accgtcagtt
     5401 catcgcccgc gtcgccgtgc tgctcttcct actggccggc ttcgcctggc agttctacga
     5461 gggctggttc gcggcgacgg gcccgtcgct cggctcgtcc tccgccggct cgctcgccgc
     5521 actgccgctg ttgctgatcc ccccggggct cttcgtcgcg ggcctcatcc tcgagctcag
     5581 ccacgcgcct caccgcggct cctgggcgct gctggccgtg ctctgcgggg cggccagcgt
     5641 ctacttcctc gccaccgcca tgaccggctc caccaccaac ctcaacctca cctattacat
     5701 cctgctcgcg gtactcggcc tcatctccat cccgcgcgcg ttctcctccg gcaagatggg
     5761 ctggagccgc atggccgcgc cgcgctacgg ctgagccacg ccggtcggcc ctactcagcg
     5821 catcagcgct ggatcagctc tacggcgttg ccgtccgggt cctcgatgaa cgccagccgc
     5881 ggcccatcgc cctcgagcag gtacggctgg cgcgtgacgc gcccgccgca ccgctcgatg
     5941 gccggcagca cagcatcgat gtcgtcggtc tcgaaggcga agtgcccgaa gcgctcaccg
     6001 ccggggtacg cgtcgtcctt gccccagttg tagacgagct cgatggcgaa gttgccctcc
     6061 tcgggctcca tgaacacgag cgtggccttg cctttggtca gctcgctgcg gcgtgactcg
     6121 tgcaggccga gctgttcgta gaacttgatc gctccctcga ggtccctggt cctggtcgcg
     6181 acgtgcacga acttcatgtg gtcctcctcc ggcgcgctcc agtatgctgc gcgcatgtcc
     6241 atcagtgcgt tcggcgacaa ggtaccccag ttcggccccg gtgtcttcgt gcacgtcgac
     6301 gcgacggtca tcggcgacgt cgtcctggag gagggcgtca ccgtgtggcc cggcgccgtg
     6361 ctgcgtggag atgtggagca gatcgtcgtc ggcgctcaca cgagcttcca ggacggggcc
     6421 atcgcccaca gcgaccccgg atttccggtg cggatcggcg ccgcatgcat catcggccac
     6481 ggcgccatcg tccacggtgc gaccatcgct accggctgcc tcatcggcat gcacgcgacg
     6541 ctgctcaacg gctgcgagat cggcgagcag agcatcgtcg ccgcgcaggc gctcgttccg
     6601 gaggggcgct cgtgcccgcc gcgatcgctc ttcatcgggg cgccggcgag ggtgatccgc
     6661 aagctcagtg acgaggacct cgcgccgctc acgggcgtcc acgagcggta ctccgctcgc
     6721 ggccgcgcgt acacggagca ggggctgggg gcggacctct ccgcgttccg tggtccggcg
     6781 gactgaccat ctgtgccccc gggtagcttg gcggcatggc cgtcatcgcc gaggcagaca
     6841 ggttcttcgc cgtcgaccgc gccgcgtggc gtgagtggtt ggcggctcac cacgaggagc
     6901 gcacgcaggt ctggctgatc ctgcacaaga agcatgtgga ggagcccagc gtgcgctacg
     6961 aagaggccgt cgaggaggcc ctgtgctggg gctggatcga cggcctcacc aacagctggg
     7021 acgagcgcag ctacgcggtg cgcttcacgc cgcgcaagcc cggcagcgtc tggagcgaga
     7081 gcaacgtcga gcgggtcacc cgcatgatcg ccgaggggcg tatgacgccc gccgggcagg
     7141 cgctcgtcga cgaagccaaa cggcgcggca cgtgggacga ggcggcgtcc ggcaggctcg
     7201 acgtgacgcc gcccgacctg gaggccgcgc tggccggcgt gcccgcggct gcggcccgct
     7261 ggaacgaatg gccggccaca ccgcggcgga tgtacgtcta ctgggtgctg gacgccaaac
     7321 ggccggagac ccgcgcgcgg cgcatcgccg acgtcgtgcg gcgcgccgcc gccgggctga
     7381 ggccgggaga gaagggctga cggcgcctgg tgggctacgc gcccaggtgc tcccggaccg
     7441 cgcggaccag ctccggcagc acctcaccgc cggggccgcg caggtagacg tcgctcgtcc
     7501 gcgtgagggc gtcgggctcc gggttgacgt cgatcagcgt cgccccggct tccttggcga
     7561 tcatcggtat ggcggcggca ggatagacga cccccgacgt gccgatgatg agcatcacgt
     7621 cgcaggccgc gccgagccgc tgggcggcgc tgatcgcacc cggggggagt ccctcgccga
     7681 accagacgac ctcgggacgc aggtagtcgc cgcactcggg gcagcgcggc ggcttctcgc
     7741 cctggtctgc gaagtcctcc cggcggtatc cgcggtgccg gccgtcgatg cagcggaagc
     7801 tgtgcatcgt gccgtgcagc tcgatcacgt gcgacgagcc ggcgcgctgg tggagcccgt
     7861 cgatgttctg ggtgatgacg acgaccttgg ggagcagtcc ctccagctcc gcgatcgcgc
     7921 ggtggccggg gttcggctct gcctcggccg cgacgccgaa gcggtgctcg taccagctcc
     7981 agacgaacgc cggatctctg cgatacgcct cgggggtggc cagctgcatc gggtcgtagc
     8041 gcgcccacag ccctgtctcg ggctcgcgga aggtggggat gccggactcc ttgctgacac
     8101 cggcgccggt gaacaccgcg agccgggccg accgggccag ggcgcgcgcc gccgtttgca
     8161 gggcgtcgtc gtcgggtcgc ggagaacggt cgctcacggg agcctcctgg tgcgagtgct
     8221 cggggccggc tgagattgta cgctagcgcg acatgagcga ggaaccacag aggacggaag
     8281 ggccggggcc ggcgccgggt ccggaggccg aggagccggc gggcggagcc gggccggccg
     8341 tgggggcgca aggcggcggc ggcgcgcacg ccggcctgcg gctgctcgcg gccgcggtca
     8401 tgctgccgtg gctcctcgcg tacgggatct tcggcgtctc ggcggtcacc cgatcggccc
     8461 aggcgtacgg cgccgggctc gagcgcatcg accccggcta cacccgcttc gtctcgcctg
     8521 gggagctcgc ctacgtcggg gcgctgctgc tggccgcgtt cgcggtgctc ctcgcctgct
     8581 ccctgctcct gctcttccgt caccgcaccg cgacggggtg gctgccggtg ctgctcgtcg
     8641 ccgccgggct cactgccggg tcggtgtggg cagccgtcca gggcgggctg caccccatcc
     8701 tctgggtcct gttcttcttc ggcctcgcct gctgcaccgc gatcgccctg gcgcgcgtgc
     8761 ttcaggtgac gcggtcgggg cggcgtggga gaatcgcctc accatgagca ccagcatctt
     8821 cgtcgggcac gccaagccac cgtccaacac cgtcagcggc cagatgtaca cgatcctcag
     8881 cgtcgtgtgc gaggtcgaca tggagaccgg cgtcatcgtc gcggccgagt tcaccgtggc
     8941 caccgagctt gccaaggact acctgaaccg cctgctggcc gggcgcaacc tcaccaccga
     9001 cgaggacgcc atcgtcgccg agctcgaggg ctgctacttc tcgggcacgc agaaggcgct
     9061 catccagtgc ttccgcgaca tggccaagcg ctaccgcgcg caggccgggc cgagccggcc
     9121 ggacgctccg gcggagccgg ccccccgggc cggctgaagc ctcctcacag atccgcccgg
     9181 cgcggcgaca cggcgcgctg ttccttggta tagtccagcg gtccgcacga gaacgcgagg
     9241 agggacccgg atcatggtcg cagtcgatgg aatcacgtca ttcgagccgc cgcgccgcgt
     9301 gctcatgggt cccggcccgt ccgacgtcca ccagcgcgtg ctcgacgcca tggcgcgtcc
     9361 caccatcggc cacctcgacc ccgagttcgt gcgcatgatg gacgagctca aggacctgct
     9421 gcgctacgcg ttccagaccg ggaaccgcgt cacgttcccc gtctccgggc ccggctcggt
     9481 cggcatggag acgtgcttcg tgaacctggt cgagccgggc gacaaggtgc tcgtctgccg
     9541 caacggcgcc ttcggcgggc ggcagctcga gaacgtggag cgcaccggcg gcgtgccggt
     9601 ggtcgtcgac gacgactggg gcacgcccgt ctcgctcgac aagctcgagt gggcgttcga
     9661 cgcccatccg gacgtccgcg tcgtctcctt cgtccacgcc gagacctcga ccggtgtgcg
     9721 cagcgacgcc caagccatcg cggcgatcgc gcacgcgcac ggcgccctgg tgatcgccga
     9781 caccgtcacc tcgctcggcg gcatccccgt gctggtcgac gagtgggggc tcgacgccgt
     9841 ctactcgggc agccagaagt gcctgagctg cacgcccggc ctctcgccgg tcaccttcgg
     9901 cgaggccgcg ctcgagaagg tcaaggcgcg cgagacgaag tgccagagct ggttcatgga
     9961 cctgaacctc gtgctgccct actgggaggg cggcgcgcgg acctaccacc acaccgcgcc
    10021 gatcaacccg ctctacggcc tgcacgagtc gctgctgatg ctgagggaag aagggctgga
    10081 ggcggcgtgg cggcggcacg ccgagctcca cgccgccctg gtcgaagggc tcgaaggcct
    10141 cggcctgagc atgttcgtcg aggccccgta ccgcctcccg cagctcaacc tcgtgagcgt
    10201 ccccgagggc gtcgacgacg cctgggtccg tgccgaggcg ctccggcgct tcggcctcga
    10261 gctgggcaac ggcctcggcg tgctggccgg caaggtctgg cgcgtcggcc tcatgggcac
    10321 ctcgtgcacg caggccaacg tcgacctctg tctggacgcg ctggggacgc tgatcccgga
    10381 ggccgcggcg aagtaggccg cggccacggc ggcaggcgct gccgtctctg cacgttgtcc
    10441 gccacacgag tgggtgctag cgttcatcta ttcaggactc actcgtggcc ggggggatcc
    10501 gcgtctgatc cggcggcctg catcggccgg cacttaggag ggcacatgac cgacgagacc
    10561 agcaagggcc ccgaaccgac cgtcgaggag ctcaaggcca aggcgtacca gcccatggag
    10621 gacgccgccg tcctccaccc cttctaccgc ggcaagatcg agaccacgct caagtgctgc
    10681 atccgcgact tccaggactt cggcatctgg tacaccccgg gcgtgagcaa gccgtgtctc
    10741 gacatcaagg acgaccccga gaaggtctac gagttcacca acaagtggaa cacggtcgcc
    10801 gtcgtcagcg acggcacgcg ggtgctgggc atgggcgaca tcggccccaa ggccggcatg
    10861 ccggtcatgg agggcaagag cctcctctac aagtacctgg gcggcgtcga cggcttcccg
    10921 ctcatggtcg acaccaagga ccccgacgag ctcatcaagt tcgtcaagct cgtgcagccg
    10981 gggctgggtg gggtgaacct ggaggacatc gcccagccga agtgcttccg catcctcgac
    11041 acgctgcgcg cggagtgtga gatccccgtg tggcacgacg accagcaggg caccgcgtgc
    11101 gtcacgctcg ccggcctcat caacgcgctc aaggtggtcg ggcggcgcat cgacgacgtc
    11161 gagatcgcct tcatcggctc gggcgccgcc aacgtcgcca tcagccgcct gatcttcgcc
    11221 tacggcgccg atccggccaa gtgccgcatc gtcgacagca agggcatcct gcacaagggt
    11281 cgcgcggaca tcgaggctgt caaagacgag tgggtcgaca agtggaagta ctgccagatc
    11341 acgaacgccg agcagcgcca gggcacgatc gcggacgcgc tcaggggcgc gcacgtgtgc
    11401 atcgcgctga gcaagcaggg ccccgacacc atcgacaagg agtggatccg ggggatggcc
    11461 cacgacccgg tggtcttcct ctgcgccaac ccgatccccg agatgtggcc gtgggacgcc
    11521 aaggaggccg gcgccgccgt ggtggccacc ggccgcagcg acttccccaa ccaggtcaac
    11581 aactcggtcg gcttccccgg catcttccgc ggcaccctgg acgtgcgcgc caggacgatc
    11641 accgacgaga tgtgcatcgc cgcggccgag gagctcgcca aggtggccga ggacaagggc
    11701 ctctccgccg actacatcat gccgaccatg gacgactggg aggtgttccc gcgcgaggcc
    11761 gcggcggtgg cgatgaaggc catcgagcag ggcatcgcgc tgcgcaccga cctcaccttc
    11821 gagcaggagg tcgcggaggc gacggcgatc atcgcccgcg cccgcggcat ggtgcaggac
    11881 cagatggcga tcggctacat cccgatgccc gagggcagtc gcgccccggc gccgaccaag
    11941 gccgcggctc gggccgtggc gcgcggcgcc ggcgactacg ccggcgagat ggccgacaag
    12001 gccgtcgaca ggatcgggcc ggcggccgac aagatcgccg cggcgacggc ggccgccatc
    12061 gacaaggcga cggacgccat caagagggcg atgaagaagg acgacggcgt caccgacgcc
    12121 gaggttgtct cggaggagcc tccggagtgg aagccgccgg ccgccgagcc tgagcctccg
    12181 gcggacgcgg agaagaccga gtagcaggac gggcggggcg gctcttccgg ccgccccgcc
    12241 ccatccgtcc gcatggggaa ggggctgtcg tgaagttctg tccgagctgc ggcgcgggtg
    12301 tggagcccgg cgccgagtcc tgcaagtcct gc
//
LOCUS       DASUMX010000323         3617 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_415893, whole genome shotgun sequence.
ACCESSION   DASUMX010000323 DASUMX010000000
VERSION     DASUMX010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3617
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415893"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(225..455)
                     /locus_tag="VFZ86_15405"
     CDS             complement(225..455)
                     /locus_tag="VFZ86_15405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643698.1"
                     /translation="MSESGPARTDPPKAGRNTADAQSSLVGGGWGDPRISASKFADAN
                     EGSRNVRRPWVWALGWVGGFVAVIAALILILR"
     gene            complement(597..1121)
                     /locus_tag="VFZ86_15410"
     CDS             complement(597..1121)
                     /locus_tag="VFZ86_15410"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX5643699.1"
                     /translation="MRRIVAYLAASVDGYIAGPGDDMGWLPEFDGDATGFHEFFSDVG
                     AIAMGRRTYEWIGEHASAWPYGDTQAYVLSGTLQAGREEHVTVTPGPVRDLAATLRDG
                     EGTAWLVGGGGLFAAFAAAGLIDLWIVTLIPVLLGGGVPLLPAGGEGYQRLKLVESRT
                     LPGGCVQLHYGPQR"
     gene            complement(1163..1912)
                     /gene="pgl"
                     /locus_tag="VFZ86_15415"
     CDS             complement(1163..1912)
                     /gene="pgl"
                     /locus_tag="VFZ86_15415"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEX5643700.1"
                     /translation="MSVEILPDGDALALRTADLFALAAQEAAAARGRFAVALSGGETP
                     RALFKLLARQQFSQKIPWRRVQLYWGDERCVPPDDAQSNYGAARDAFIRHVPITDANV
                     HRMRGEDPPEEAAQAYDAELRGLAAQERPRAEVPVFDLVLLGLGTDGHTASLFPHSDA
                     LAVEDRFCVATTAPDGSPRLTVTAPVINAARRVWFLVGGAKKAGMVAEVLEGLRVPNA
                     VPAQGVAPVHGALTWFLDEAAAAELSPDARG"
     gene            complement(1909..3579)
                     /gene="zwf"
                     /locus_tag="VFZ86_15420"
     CDS             complement(1909..3579)
                     /gene="zwf"
                     /locus_tag="VFZ86_15420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEX5643701.1"
                     /translation="MTVDQDPAGHLSYDELDDGDEGGIRVTGRSRASGAPSPFGGERH
                     PDPCSLVMFGVTGDLAHRKLIPALYDLGCHGVLPLGTTLVGYGRQEMTDDQFRDLMRR
                     AIDDHYGEEVVDGSACDRLLMEPRYVHGQFDDPEGFKRLAAVLDELDGKGARGNRMFY
                     LATPPSQFEVLVRQLGDAGLARRGAYGGEDAPLAGGRPGWTRIVIEKPFGRDLATARE
                     LDRVIAQVFDERQVYRIDHYLAKETVQNLLVLRFANVIFEPVWNRRYVDFVEITAAET
                     LGVEHRGPYYEEAGALRDMITPHLIQLFSLVAMEPPAAFDADGVRDEKMKVLRAVRPI
                     PPHSVGDWAVRAQYVQGRVDGEDVPAYRSEERVAPDSYTETYAALKLVIDNWRWQGVP
                     FYLRTGKRMPRRVTEIAIHFKRPPVLLFKDAGAGGGVQPNVLVLRVQPDEGFSLSIES
                     KQPGHALALQPVAMDYSYGVTLHELPFSAYETVLVDAMEGDMTLFTRGDQAEEAWKIV
                     GPVLEAWAERPQRDISIYEAGSWGPEAADALIAHDGNSWRRPTKGDET"
BASE COUNT          477 a         1298 c         1313 g          529 t
ORIGIN      
        1 acccctcgaa aggggccgct ggcggtctgc tctctgttgc gctttccctc gggtttcccc
       61 ggctggccgt tagccagcgc cctgctctgt ggtgtccgga ctttcctcga gcggtgcctc
      121 gcgggctccg aagagcggcg cgacgcggtc cgcccgcggc cacctggctt gctccagaag
      181 tgtaccaccg gggcgctcag ctcagaccga tgcctgaacc gcccttagcg caggatgagg
      241 atgagcgccg cgatgacggc gacgaagccg ccgacccagc cgagcgccca gacccacggt
      301 cgccgcacgt tccgcgaccc ctcgttggcg tcggcgaact tcgaggcgga gattctcggg
      361 tcgccccacc caccgccgac cagggaggac tgggcgtctg ctgtgttcct tccggccttg
      421 ggagggtcgg tgcgtgccgg accggactcg ctcatggcgg atccctctcg tcggccgtgc
      481 gcgccccacg gcgcgtcgcg gcggggcttc gccccgtggg gagatagtac cctgacttcg
      541 tcgccatcca ccgtgtggcc ggagacgacc ggcggcggac tcgaccttcg cccggttcag
      601 cgctgtgggc cgtagtgcag ctgaacgcag ccgccaggga gcgtgcggct ctcgacgagc
      661 ttcaggcgct ggtagccctc gccgccggcc ggcagcagcg gcacgccgcc gccgagcagc
      721 accgggatca gcgtgacgat ccagaggtcg atgaggccgg cggcggcgaa ggcggcgaac
      781 aggccgccgc cgcccaccag ccacgccgtg ccctcgccgt cgcggagcgt cgcggcgagg
      841 tcgcggaccg ggccgggagt gacggtgacg tgctcctccc ggcccgcctg caacgtaccc
      901 gagagcacgt aggcctgggt gtcgccgtag ggccaggcac tcgcgtgctc gccgatccac
      961 tcgtaggtgc gccggcccat ggcgatggcg ccgacgtcac tgaagaactc gtggaagccg
     1021 gtcgcgtcgc cgtcgaactc cggcagccag cccatgtcgt cgccggggcc ggcgatgtag
     1081 ccgtccacgc tggcggcgag ataggcgacg atccggcgca tggcggctcg attcccgcgg
     1141 cgacgcggac gctaacgccg cctcagccgc gtgcgtccgg gctgagctcc gccgcggcgg
     1201 cttcgtccag gaaccacgtg agcgcgccat gcacgggcgc gacgccctgc gcgggcacgg
     1261 cgttgggcac gcgcaggccc tcgagcacct cggcgaccat gccggccttc ttggcgccgc
     1321 cgacgaggaa ccagacgcgg cgcgcggcat tgatcacggg cgcggtcacg gtgagccggg
     1381 gtgaaccgtc gggcgccgtg gtcgcgacgc agaagcggtc ctcgaccgcg agcgcgtcgc
     1441 tgtgcgggaa gagcgaggcc gtgtggccgt cggtgccgag gccgagcagc accaggtcga
     1501 acacggggac ctcggcgcgc ggccgttcct gcgcggccag gccgcgcagc tcggcgtcgt
     1561 acgcctgcgc ggcctcctcc ggcgggtcct cgccgcgcat ccggtgcacg ttggcgtccg
     1621 tgatcggcac gtgcctgatg aacgcatcgc gcgctgcgcc gtagttgctc tgcgcgtcgt
     1681 ccggaggcac gcagcgctcg tctccccagt agagctgcac gcggcgccag gggatcttct
     1741 ggctgaactg ctggcgagcc agcagcttga agagggcgcg cggcgtctcg ccgccgctga
     1801 gcgcgacggc gaagcggccg cgcgccgccg ccgcctcctg ggcggcgagg gcgaagaggt
     1861 cggcggtgcg cagcgcgagc gcatcgccgt cgggcaggat ctcgacgctc atgtctcgtc
     1921 ccctttcgtg ggccggcgcc aggagttgcc gtcgtgggcg atgagcgcgt ccgccgcctc
     1981 gggcccccag ctgccggcct cgtagatcga gatgtcccgc tgcggccgct cggcccacgc
     2041 ttcgagcacg gggccgacga tcttccaggc ctcctcggcc tggtcgccgc gcgtgaacag
     2101 ggtcatgtcg ccctccatgg cgtccacgag cacggtctcg taggcgctga agggcaattc
     2161 gtgcagggtg acaccgtagg agtagtccat ggccacgggc tgcagggcga gcgcgtggcc
     2221 cggctgcttc gactcgatgc tgagcgagaa gccctcgtcc ggctgcacgc gcaggacgag
     2281 gacgttcggc tgcacgccgc cgcccgcgcc cgcgtccttg aagagcagca cgggcggccg
     2341 cttgaagtgg atggcgatct cggtcacgcg ccgcggcatg cgcttgccgg tgcgcaggta
     2401 gaagggcacg ccctgccagc gccagttgtc gatcaccagc ttgagcgccg cgtacgtctc
     2461 cgtgtaagag tccggcgcga cccgctcctc ggagcggtag gccgggacgt cttctccgtc
     2521 gacccggccc tggacgtact gcgcgcgcac cgcccagtcc cccaccgagt gaggcgggat
     2581 gggccgcacc gcgcgcagca ccttcatctt ctcgtcgcgc acgccgtcgg cgtcgaaggc
     2641 ggccggtggc tccatcgcga ccagggagaa cagctggatg aggtgcggcg tgatcatgtc
     2701 gcggagcgcg ccggcctcct cgtagtaggg gccgcggtgc tcgacaccga gcgtctccgc
     2761 ggccgtgatc tccacgaagt cgacgtagcg ccggttccac accggctcga agatgacgtt
     2821 ggcgaagcgc aggaccagaa ggttctggac cgtctccttt gcgaggtagt ggtcgatgcg
     2881 atagacctgc cgctcgtcga acacctgggc gatcacgcgg tcgagctcgc gcgcggtcgc
     2941 gaggtcgcgg ccgaacggct tctcgatgac gatccgcgtc catcccggcc gcccgccggc
     3001 gagcggcgcg tcctccccgc cgtacgcgcc gcggcgcgcg agcccggcgt cgccgagctg
     3061 tctgacgagc acctcgaact ggctcggcgg cgtggccagg tagaacatgc ggttgccgcg
     3121 cgcgcccttg ccgtccagct cgtcgagcac ggcggcgagg cgcttgaacc cctcgggatc
     3181 gtcgaactgc ccgtgcacgt acctcggctc catgagcagc cggtcgcacg ccgagccgtc
     3241 gacgacctcc tcgccgtaat ggtcgtcgat ggcccggcgc atgaggtcgc ggaactggtc
     3301 gtcagtcatc tcctggcggc cgtacccgac cagggtggtg cccaacggca gcacgccgtg
     3361 gcagccgagg tcgtagagcg ccgggatgag cttgcggtgc gccaggtcgc cggtgacgcc
     3421 gaacatgacc aggctgcagg ggtccgggtg ccgctcgccg ccgaacggcg agggcgctcc
     3481 ggacgcccgg gagcggccgg tgacgcggat gccgccctcg tcgccgtcgt cgagctcgtc
     3541 gtagctcagg tgcccggcgg gatcctggtc caccgtcact ccttctcctt gacggcgtgg
     3601 ccgccgaact cgttgcg
//
LOCUS       DASUMX010000324         6422 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_990276, whole genome shotgun sequence.
ACCESSION   DASUMX010000324 DASUMX010000000
VERSION     DASUMX010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 6422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6422
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_990276"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1399
                     /locus_tag="VFZ86_15425"
     CDS             <1..1399
                     /locus_tag="VFZ86_15425"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEX5643702.1"
                     /translation="DTAVALIRDLLPHLRETDLLTLAVGACGIAGMVVLERLLPKVPA
                     ALLVLVGSIAASVALGLQTRGVAVVGELPAGLAGPSLPGVGLEAVPQLMVGALAIALV
                     AFAEAIGPAEEFAREHGARVDADRELIALGAANAGAGLFGGFPIGSSLSKSAANDRAG
                     ARTPASLITAAAATALVALFLTPLFEPLPEATLGAIVVVAVAGMMKVGKMKRLWRLRR
                     AEFWLAVIALAGVLVIPTLPALALAVVVSLGMLVWRASQPRMTLLGRARGGLEPVDLR
                     TAPDAAIPGLLIVRPDQMLFFANVAPVRSGIVQAVADTDPRPTVVLLDLGLTPEIDVP
                     VVEALEQLHQRLGADGIELWLCHLLPAARDLLDRAGVLAAVGPGRIHPRVLDGIVAFA
                     LRSPAGKDRIAALADVRALIRERAAQPGLSADAAELLTVLEERLSLELSAATGGSERV
                     ILPTTSDSPSGRGPT"
     gene            1396..2424
                     /locus_tag="VFZ86_15430"
     CDS             1396..2424
                     /locus_tag="VFZ86_15430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006542799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HEX5643703.1"
                     /translation="MTDLRPKRIAEFIEPLRVTPGRRVTLAKHFDPSYKAGFLDKDEG
                     EELLARGVELLTEYQERLAAQDTHGVIIVLQALDAAGKDGTIRHVMSGVNPQGVSVHS
                     FKAPSSLELDHDYLWRFACNLPRRGEIGIFNRSYYEEVLVVRVHPEILLNQQLPARAR
                     GRDVWKKRYRDINAWEQHLTDNGFTIVKLFLNLSKEEQRTRFLRRIDLPDHNWKFSAA
                     DVKERSYWDDYQKAFSEMLSATSTEWAPWYVLPADHKWFARICASAIIADALIKIDPK
                     FPKVTPEAHDALMETKGLLEAEAPDGAEADPFAVEELEDAKKAAKADRQDGKKGKKKR
                     GKKAGKAK"
     gene            complement(2441..3694)
                     /locus_tag="VFZ86_15435"
     CDS             complement(2441..3694)
                     /locus_tag="VFZ86_15435"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HEX5643704.1"
                     /translation="MSRTARKWVLILCLALVVIGAVAAVVVLMGGVTVPDVTGKTQAD
                     ATRELEDAGLDVGEVTQASDALAAEGTVLVQDPAAGTEVDEGSAVALTLSSGPGTAAV
                     PDVVGMTRAAAEAALSAAGFIAASALQYDVTAPVGEVVDQLPAGGEPAAPGSQVGLLV
                     SRGRPEVAVSVPDVTGMAQDEATAALTDAGLAAVAVEAYVADVPAGAVAEQEPAAGDR
                     VAPLSEVLITVSLGEGTTSVQVPDVTGKRQSDASSELEAAGLVVTAAEAWSSDVAEGR
                     VIAQAPEAGTMVEEGGDAGILVSLGPLPTPSPSPSPTESGGASPSASPGATPPPDGAD
                     PPEQPPQLMVTVPDLVGMDLEQAEEELTGLGLKPVPLEVPSATTAPGVVYAQLPEAGT
                     EVPKTYPVLLLVSIGVQVDTLPAGE"
     gene            complement(3722..5281)
                     /locus_tag="VFZ86_15440"
     CDS             complement(3722..5281)
                     /locus_tag="VFZ86_15440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012691361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5643705.1"
                     /translation="MALDSSVMNVSIATVAEDVGTTVTGIQTAITLYTLVMACLMIPG
                     GKIGSIIGRRRAFSIGCVIYAAGSMTTALAPSLPVLILGWSFLEGIGAALIMPAIVAL
                     VAGNFEVAARPKAYGLIAAAAAMAIAVGPLIGGLVTTYASWRYVFVGEVIVIVVLLFF
                     ARRVKDAPVEKRPRLDVVGAVLCAIGLALIVLGVLRSSEWGWIVPKEGGTAWLGLSPT
                     IWLILAGGLVLWLFLRWLSRLDRAGKEPLVDPALFRVHQLTGGLVMFFLQFLLQMGIF
                     FIIPLFLSVVLGMTASQTGVRLLPLSAGLLLAAVGVPKRWPKASPRRVCRVGLFLILA
                     GIVLLLGGIDLDASAAVVLVPMLLIGLGIGALASQLGAVTVSAVPDEKASEVGGLQNT
                     SSQLGSSIGTALAGSIMIAVLTASFLTGIQNNPNVPEEVKSQATVELAPGIPFISDAD
                     LEQAMKDAGQSEEVTQAAVDANREARIDGLDAALAILAVIAVVALFLTSGVPKIPVAG
                     AGAQPAQAQGP"
     gene            complement(5459..6118)
                     /locus_tag="VFZ86_15445"
     CDS             complement(5459..6118)
                     /locus_tag="VFZ86_15445"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HEX5643706.1"
                     /translation="MVEPPKPEKIKHRKNRLEAFSDGVFAIAITLLVLEIAVPAISDG
                     VGSGEVIRGIGEEWPTYLAYFISFMTIGAVWIEHSALVDALDHIDGVFMRINLVLLLF
                     CAFLPFPTRMMSEYHGEIDAERVAVVFYGAVLLAMVLMLIVLGRHAEREGLFGDDLVD
                     ERKEEARVKYQLMPSLIAYIVAAFLGFFVPRLGISIYLLVAIWLAIPLRDVRKLFGRS
                     A"
BASE COUNT          988 a         2309 c         2239 g          886 t
ORIGIN      
        1 cgacacggcg gtcgcgctca tccgcgacct tctccctcat ctgcgggaga ccgacctgct
       61 gaccctcgcg gtcggcgcct gcggcatcgc cgggatggtc gtgctggagc gcctgctgcc
      121 gaaggtgccg gccgcgctgc tcgtgctcgt cggctccatc gcggcgtccg tcgccctcgg
      181 cctccagacg cggggcgtcg cggtcgtcgg cgagctgccc gcgggcctcg ccggcccctc
      241 gctgccggga gtggggctgg aggccgtgcc tcagctgatg gtcggcgccc tcgccatcgc
      301 cctggtcgcc ttcgcagagg cgatcggacc ggccgaggag ttcgcgcgcg agcacggcgc
      361 cagggtcgac gccgaccggg agctcatcgc gctcggcgcg gccaacgcag gagccggcct
      421 cttcgggggg ttccccatcg gctcgtcgct gtccaagtcg gccgccaacg accgcgccgg
      481 cgccaggacg ccggcctcgc tcatcacggc ggccgcggcg accgccctgg tggcgctgtt
      541 cctgacgccc ctgttcgaac cgctgcccga ggcgacgctc ggggccatcg tcgtcgtggc
      601 ggtggccggg atgatgaagg tgggcaagat gaagcgactc tggcgactgc gccgcgcgga
      661 attctggctc gccgtgatcg ccctcgcagg tgtgctggtc atcccgacgc tgccggcgct
      721 cgcgctcgcc gtcgtcgtct cgctcggcat gctggtctgg cgcgccagcc agccgcgcat
      781 gacgctgctc gggcgggccc gcggcggcct ggagccggtg gacctgcgga cggcgccgga
      841 cgcggccatc ccgggactgc tcatcgtgcg tcccgaccag atgctcttct tcgccaatgt
      901 ggccccggtg cgcagcggca tcgtccaggc tgtcgccgac accgacccac gtcccacggt
      961 cgtcctgctc gacctgggac tcacgcccga gatcgacgtc cccgtcgtgg aggcgctcga
     1021 gcagctgcat cagcgcctcg gcgccgacgg catcgagctc tggctgtgcc acctcctccc
     1081 cgcggcgcgc gacctcctgg accgcgccgg ggtgctcgcc gccgtcgggc ccggacggat
     1141 ccacccccgg gtgctggacg ggatcgtcgc gttcgcactc cgctcgccgg ccggcaagga
     1201 ccgcatcgcc gccctcgccg acgtgcgcgc cctcatccgc gagcgcgcgg cgcaaccggg
     1261 cctgagcgca gacgccgccg agctcctgac cgtgctggag gagcggctct ctctcgagct
     1321 ctcggccgcg accgggggaa gcgagcgtgt catactcccg accacgtcag actctccaag
     1381 cggaaggggc ccgacatgac agacctgagg ccgaagcgca tcgcggagtt catcgagccg
     1441 ctgcgcgtca ccccgggccg gcgggtgacg ctggccaagc acttcgatcc ctcctacaag
     1501 gcggggttcc tggacaagga cgagggcgaa gagctcctgg cgcgcggcgt cgagctgctg
     1561 accgaatacc aggagcgcct ggcggcgcag gacacccacg gcgtcatcat cgtgctgcag
     1621 gcgctggacg ccgccggcaa ggacggcacc atccgccacg tcatgagcgg cgtgaacccg
     1681 cagggcgtgt cggtgcactc gttcaaggcg ccctcctccc tcgagctcga tcacgactac
     1741 ctgtggcgct tcgcctgcaa cctcccccgg cgcggcgaga tcggcatctt caaccgctcc
     1801 tactacgaag aggtcctggt ggtgcgcgtg cacccggaga tcctcctgaa ccagcagttg
     1861 ccggcgcggg cgcgcgggcg ggacgtctgg aagaagcgct accgcgacat caacgcctgg
     1921 gagcagcacc tcaccgacaa cggcttcacc atcgtcaagc tcttcctcaa cctctccaag
     1981 gaggagcagc gcacgcgctt cctgcggcgc atcgacctgc cggaccacaa ctggaagttc
     2041 tccgccgccg acgtcaagga gcgcagctac tgggacgact accagaaggc cttctccgag
     2101 atgctctcgg cgaccagcac cgagtgggcg ccctggtacg tgctcccggc cgaccacaag
     2161 tggttcgcgc gcatctgcgc ctcggcgatc atcgccgacg cgctcatcaa gatcgacccg
     2221 aagttcccca aggtgacgcc cgaggcgcac gacgcgctca tggagaccaa ggggctgctg
     2281 gaggccgagg cccccgacgg cgccgaggcc gacccgttcg cggtcgagga gctcgaggac
     2341 gccaagaagg cggcgaaggc cgaccgccag gacggcaaga agggcaagaa gaagcgcggc
     2401 aagaaggccg ggaaggcgaa gtagccgcgc cggcccgccg tcactcgccg gcgggcagcg
     2461 tgtcgacctg cacgccgatg gacacgagca gcagcacggg gtaggtcttg gggacctccg
     2521 tgcccgcctc gggcagctgc gcgtagacca cgccgggcgc cgtggtggcg ctgggcacct
     2581 ccagcggcac gggcttcagg ccgaggcccg tgagctcttc ctcggcctgt tcgaggtcca
     2641 tgccgacaag gtcgggcacc gtcaccatca actgcggcgg ctgctcgggc gggtcggcgc
     2701 catcgggcgg aggcgtcgcg ccgggagacg cggacggcga cgccccaccg gactcggtcg
     2761 gtgacggcga cggagaaggg gtgggcagcg gcccgagcga gacgaggatg ccggcgtcgc
     2821 caccctcctc gaccatcgtc ccggcctccg gcgcctgggc gattacccgg ccctcggcga
     2881 cgtctgagct ccaggcttcg gccgccgtca cgacgagccc ggcggcctcc agctcgctgc
     2941 tcgcgtcgga ctgcctcttc cccgtcacat cggggacttg cacgctcgtc gttccctcgc
     3001 cgagagacac ggtgatcagc acctcggaga gtggcgccac gcggtcgccg gccgcaggct
     3061 cctgctcggc cacagctcct gcgggcacat cagccacgta ggcctcgacg gcgacggcgg
     3121 cgagtcccgc gtcggtgagc gccgcggtcg cctcgtcctg ggccatgccg gtcacgtccg
     3181 gcacgctcac ggcgacctcc ggccggcccc tggacacgag caggccgacc tgggacccgg
     3241 gcgcggccgg ctcgccgccg gccggcaact ggtccacgac ctcgccgacc ggcgccgtca
     3301 cgtcgtactg cagggcgctg gccgcgatga agccggccgc ggacagagcc gcctcggcag
     3361 cggcccgggt catgccgacg acgtccggca ccgcggcggt cccaggcccg gacgagagcg
     3421 tcagcgcaac agcgctgccc tcgtccacct cggtgcctgc ggccgggtcc tgcacaagca
     3481 ccgtaccctc cgccgccaag gcgtcgctcg cctgtgtgac ctcgccgacg tcgagcccgg
     3541 cgtcttcgag ctccctggtc gcgtcggcct gcgtcttgcc cgtcacgtcg ggcacggtga
     3601 cgccgcccat gagcacgacg acggccgcca ccgcaccgat caccacgagc gcgagacaca
     3661 ggatgaggac ccacttgcgc gctgttctgc tcatgccgct ccttccgcgg gtcgcttccg
     3721 gtcagggacc ctgggcctga gcgggttggg cgcctgcacc ggcgactggg atcttcggca
     3781 cccccgaagt caagaacagc gcgacgacgg cgatcacggc aaggatcgca agcgcggcgt
     3841 caagcccgtc gatccgcgcc tccctgttgg cgtcgacggc cgcctgtgtg acctcttcgg
     3901 actgtcccgc gtccttcatc gcctgctcga ggtcggcgtc ggagatgaac gggatccccg
     3961 gggccagttc gacggtcgcc tgggatttca cttcctccgg cacgtttggg ttgttctgga
     4021 tgccggtcag gaacgaggcg gtcagcacgg cgatcatgat cgacccggcc agcgccgtac
     4081 cgatcgacga gccgagctgg gacgaggtgt tctgcaggcc gccgacctcg gaggccttct
     4141 cgtcgggcac cgcggacacg gtcacggccc ccaactggga ggcgagcgcg ccgatgccga
     4201 ggccgatgag cagcatcggg acgaggacca cggcggcgct ggcgtcgagg tcgatcccgc
     4261 ccagcagcag gacgatgccg gccaggatga ggaatagccc cacgcggcac acgcgccggg
     4321 gagacgcctt gggccaccgc ttgggcacgc ccacggcggc gaggagcagg ccggcggaca
     4381 ggggcagcag gcgcacaccc gtctgtgagg cggtcatccc gagcaccacc gagaggaaca
     4441 gcgggatgat gaagaagatc cccatctgca gcaggaactg caggaagaac atgacgaggc
     4501 cgccggtcag ctgatggacg cggaagagcg cggggtcgac cagcggctcc ttgcccgcgc
     4561 ggtcgaggcg cgacagccag cggaggaaga gccagaggac gaggccgccg gcgaggatga
     4621 gccagatcgt cggcgacagg cccagccacg cggtcccacc ctccttgggc acgatccagc
     4681 cccactcgga ggagcgcagc acgccgagca cgatgagcgc cagaccgatg gcgcagagca
     4741 cggcgccgac gacgtcgaga cgcgggcgct tctccaccgg ggcgtccttc acgcggcgcg
     4801 cgaagaagag gagcacgacg atgacgatga cctcgccgac gaacacgtag cgccaggagg
     4861 cgtacgtcgt gacgaggccg ccgatgagcg gcccgacggc gatcgccatc gccgccgccg
     4921 cggcgatgag gccgtaggcc ttcggccggg ccgcgacctc gaagttcccg gccaccagcg
     4981 cgacgatcgc cggcatgatc agcgcggcgc cgatgccctc gaggaacgac cagccgagga
     5041 tgaggaccgg gaggctcggc gccagcgccg tggtcatcga gccggccgcg tagatgacgc
     5101 agccgatgga aaaggcgcgg cggcggccga tgatgctgcc gatcttgccg ccggggatca
     5161 tcaggcaggc catcaccagc gtgtagaggg tgatggcggt ctggataccg gtgaccgtgg
     5221 tgcccacgtc ctcggccacg gtcgcgatcg agacgttcat gaccgagctg tcgagagcca
     5281 tgatgaactg gccggtcgcc agcacgaaga ggacgaagcc cgcggacgct gcggcgaccg
     5341 ccttggcgcc gtgcttgcct gccgccgccc ctccgcccgt tgccccggtc gtcgtgttct
     5401 cggccatcag ttcccccctc ttcagtctgt cgccgtaagg ctcagtgtca cccagcggtc
     5461 aggcggagcg gccgaacaac ttgcgcacgt cccggagcgg gatcgccagc cagatggcga
     5521 ccagcaggta gatcgagatg cccagccgcg ggacgaagaa ccccaggaag gcggccacga
     5581 tgtaggcgat gaggctcggc atgagctgat acttgacccg agcctcctcc ttgcgctcgt
     5641 cgacgaggtc gtcgccgaag aggccttcgc gctcggcgtg acggccgagc acgatcagca
     5701 tcagcaccat ggcgaggagg actgcgccgt agaacacgac ggcgacgcgc tcggcgtcga
     5761 tctctccgtg gtattcgctc atcatgcggg tcgggaacgg caggaacgcg cagaacagca
     5821 gcagcaccag gttgatgcgc atgaacacgc cgtcgatgtg gtcgagtgca tcaaccaggg
     5881 cgctgtgctc gatccaaacg gcgccgatgg tcatgaacga gatgaagtag gcgaggtagg
     5941 tgggccattc ctcgccgata ccgcggatga cctcgccgct cccgacgccg tcgctgatcg
     6001 ccggcacggc gatctcgagc acgagcagcg tgatggcgat ggcgaagacg ccgtcgctga
     6061 acgcctcgag gcggttcttc ctgtgcttga tcttctcggg cttgggcggc tcgaccatct
     6121 cgactccccc atccgcgccg gccgactccc ctcggggccg gcgctgatcc tgcagagaac
     6181 tgacggcggg cgggcggccc ttccggcctc ccgcccgccg ccactcaccc ggacgcgact
     6241 ccgctcaggc ggtggcttcc ttcttgtggt gcttggcgcc aaaggcgtgc ttgagctgct
     6301 cttcctgctc ggcgctgagg ttggaacgca cgagtgtggc gttcgtgccc tcgatagcgg
     6361 cgaggaactt gtcctcggtc agctcctgga tgacgaggaa ccagccggcc ttgcccggcg
     6421 gc
//
LOCUS       DASUMX010000325        12737 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1423097, whole genome shotgun sequence.
ACCESSION   DASUMX010000325 DASUMX010000000
VERSION     DASUMX010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 12737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12737
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1423097"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VFZ86_15450"
     CDS             <1..318
                     /locus_tag="VFZ86_15450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydromethanopterin dehydrogenase"
                     /protein_id="HEX5643707.1"
                     /translation="AVLEGACAVLCTGVEGVQLVPESLWREHPTLRVLADVNAVPPLG
                     VEGTKATWNGKDIGDKLLFGALGIGDLKMKVHKRSVARLFERNDLVLDAEGIMESAKE
                     LAG"
     gene            318..1511
                     /locus_tag="VFZ86_15455"
     CDS             318..1511
                     /locus_tag="VFZ86_15455"
                     /inference="COORDINATES: protein motif:HMM:NF014686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX5643708.1"
                     /translation="MRTLVLGVSTRAMVESAVRGGHDVVAVDFFGDRDQALVAESYAL
                     ERDLGLPPTAEGLGEAARRVAAEAVVYGANLENHAAVVARLAGEHEVLGNPPEVLREV
                     RDWALLRRACEESGIAHPVTLLPGEEAGAVTGRWLRKRVRSGGGHGVGRWDGRPLDED
                     HVLQSEVDGVPASVAFVADGRDCRILGLSEQLIGLAALGSSGFGWCGNILPLGLSPAE
                     SAAANAEVRRAASTLTRRFGLRGVNGMDVVIGRDRRGAVRPHVVEVNPRYSGSMELLE
                     DAGGVNVFSAHVDACAGRLPDAGAARSAAPEPVGKAIVYARRRVTTPDTGAWLARGVR
                     DVPRSRETIAAGHPICTVAAAGRDRRECLAGLLARAAEVYAESEARKEERRERPTHLD
                     HRTYA"
     gene            1471..1824
                     /locus_tag="VFZ86_15460"
     CDS             1471..1824
                     /locus_tag="VFZ86_15460"
                     /inference="COORDINATES: protein motif:HMM:NF013717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HEX5643709.1"
                     /translation="MSDRLTLITGRTRKQAIGMHKGKASAEYLEATALVEMNPDDMTR
                     LDVSEGGHVLLRTDDGEVELTARAGTVPAGLVFVPMGTSANKLIGTETLGTGMPCFKG
                     LQVDVTPLAGEEVSP"
     gene            1821..3089
                     /locus_tag="VFZ86_15465"
     CDS             1821..3089
                     /locus_tag="VFZ86_15465"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit B"
                     /protein_id="HEX5643710.1"
                     /translation="MSTTVENVACPFCGCLCDDIIVETEGTKILKVKLACSSGQGIFM
                     DYDPRPRRPLVHGSEVEWSEAVAEAARILSAADSPLIYGLSSSASEAQRKAVALADKI
                     GAVIDSTSSVCHGPTGLAMQAVGEPTCTLGEVRDRADLVVFWGCNPDASHLRHFPRFS
                     LTAKGTLTPNGRSDRTMYVIDVRPTGSSKKADHFLQIEVGSDYEVLTTLRLLVRGGEP
                     SSDVVGGVPVAELKELAERMKSCRYGVAFMGMGLTMAPARQLNVAELFTLVAELNDHA
                     KFSVIPMRGHGNVTGADQVLSWTTGYPFAVSFARGYPQFMPGEFTAVDMLARKAADAA
                     VIIASDPGAHFPAAAAAHLERIPTIVFDHGPSVVAAKADVLFPTAAYGVDAGGTAYRM
                     DNVPIPLTQVIETDRPTDEEILDRIIEAVD"
     gene            3089..4720
                     /locus_tag="VFZ86_15470"
     CDS             3089..4720
                     /locus_tag="VFZ86_15470"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit A"
                     /protein_id="HEX5643711.1"
                     /translation="MLGIIGGRVYDPQNDKAGEIGDIWVKDGRIVPAAEVDREKAEII
                     DAAGLVVMAGGVDIHAHIVGGEVNAGRKLRPEDHRGKERAHSGSLRAGAGGTVPSTYA
                     TGQLYSELGYTTVMQASTAPLMARHTHEELEDTPNVDNGAFMVMGNNHIVMQSIADKQ
                     FDRARDYVAWLLQATRGYAIKLVNPGGVENWKWGRNVAELDDEVIGYGVTPRQIMETL
                     ATIADDLGLPHAAHIHGINLGRHTSARTSIETIKALDGHRAHLCHLQFLSYGSGKGHV
                     HKSEAVAVAEAVNASPKLTVDVGQVVFGRATTMTCDGPLQHTLHNTAGGRWMNDDVEG
                     ESGGGIVPIEYKPGNPINATMWLTGLELFLLIDDPWKVFLTTDHPNAGTFSNYPQIIR
                     LLMDRGYREEMIATLHPRARKGALLAELDREYSLGDIAVITRSGPARALGLKDKGHLG
                     VGADADIAVYEERDDKEAMFARPRYVFKAGELVARDGAVLKQVAGRTYSVAPDYDPSI
                     EDDLRAHFKAAYTVSYDNYAMLSGDGARIETVACG"
     gene            4732..5709
                     /gene="mptA"
                     /locus_tag="VFZ86_15475"
     CDS             4732..5709
                     /gene="mptA"
                     /locus_tag="VFZ86_15475"
                     /EC_number="3.5.4.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase MptA"
                     /protein_id="HEX5643712.1"
                     /translation="MDADRACRPRRDTQQARPAVAVALSRAGVTNLRRIIAMTDGGKA
                     ELFYAVLDLYADLSPDQAGVHMSRFSEVVEDLAEGLTRKPFADIESLARRMAEDVVET
                     QGALRAEVHIRAQSPITKVTPVTKRPTEQLYTLVGIAAATRERTRLLVGVEVNGLTVC
                     PCAQDMVREHSVETLVEEGYAEQEAERIVGLLPMASHNQRGRGTLLMGSDQRLRAEDL
                     LRIAESSMSSEIYELLKRPDELAVVEKAHRNPRFVEDVVRQMLASVCKEYEHLDDDTF
                     VLARQENYEGIHEHNAFAERFGTLAEIRPEIAGRAFARQHTSQSEWLYS"
     gene            5729..7081
                     /locus_tag="VFZ86_15480"
     CDS             5729..7081
                     /locus_tag="VFZ86_15480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6513 domain-containing protein"
                     /protein_id="HEX5643713.1"
                     /translation="MVDTLFVTGRLAADALRATLARLELDFDYEVAELQGSVAALMRT
                     EWIARRLPADCGCARVMIPGLCRGDLGLIEERVGVPVVRGPKDLKDLPQHFGQERVLE
                     GYGEYHVRIIAEIVEAWRLPLPEIIAQAEYFRASGADVIDLGCPPMEEFPGVGEVVLA
                     LRERGLAVSIDTFHTATIREADEAGVDLLLSVNGQNLDIVPDLRCKVVVIPDFGEGLD
                     SLERNVDRVAELGAAYVIDPILDPISFGFTSSIERYCEVRRRHPEAEVLMGLGNLTEL
                     TDADSVGVNAVMAGVIEELHIDYVLTTEVISWARGAVRELDHARKLMHYAVGNRTLPK
                     NLDDALVALKDPPFESYSEAELRDMQHQVRDRNFRIFADGEAITVFNRALFLRGTDPR
                     ELFAALGVEEASHAFYLGRELERASLAVRLGKKYVQESPLRWGYLGADDDADPAEEPA
                     "
     gene            7078..8208
                     /locus_tag="VFZ86_15485"
     CDS             7078..8208
                     /locus_tag="VFZ86_15485"
                     /inference="COORDINATES: protein motif:HMM:NF018968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1464 family protein"
                     /protein_id="HEX5643714.1"
                     /translation="MTRVVGIDPGTVSLDLCGRDGDAVFLDESFPTATIGADPGMLVE
                     LLQGARPLDLVVGPSGYGLPWTDVRDVGAGELALLFLADAADGPRGTILGGMGRLVEA
                     LRVSGLPVCFSPSVAHLPTVPAHRKVNRIDMGTADKLCAVALGVWDQARRLGLPCSAT
                     SFVFVEAGGAFTAVIGVDGGAVVDGAGGTSGAPGFRAMGAMDGELAYLLRDFSKDVLA
                     SGGAAWVCGDPDCTPGDLVRAAAAGGPAAVALDALLEGIEKQVAAQVTVLGTPREILL
                     SGRLSRVPEIRRLLAERLSRFAPVCDVGGFAAVASEAAQGAALLGQGLAGQAGLRDLV
                     EALQVRSAAGSVLDHLYVSETESLRRRHPSAGRAPAPFWERS"
     gene            8215..9102
                     /gene="fhcD"
                     /locus_tag="VFZ86_15490"
     CDS             8215..9102
                     /gene="fhcD"
                     /locus_tag="VFZ86_15490"
                     /EC_number="2.3.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018174249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran--tetrahydromethanopterin
                     N-formyltransferase"
                     /protein_id="HEX5643715.1"
                     /translation="MHLNGVEIEDTFAEAFGMWGSRLIVTAETLEWARTAAAAATGFA
                     TSIIGCGCEAGVNELGPDEETPDGRPGIAVLVFATSRSNMESVLLARVGQAIMTSATS
                     ACYDGLGGDERLKVGGKLRYFGDGFQRSKLLDGRRLWRVPVMDGEFVVEESFGVAKGV
                     GGGNFFMMGEDLEAALTAAEAAVAAMRSVPGAIMPFPGGVVRSGSKVSSRYKGQNVSS
                     NTPYCPTLRAQEQTALREGENAVLEIVIDGLDEQAVREAMRVGITAACLPGVTRISAG
                     NYGGSLGQFHFALRELVTA"
     gene            9149..9997
                     /locus_tag="VFZ86_15495"
     CDS             9149..9997
                     /locus_tag="VFZ86_15495"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR03122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018493 - formylmethanofuran dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit C"
                     /protein_id="HEX5643716.1"
                     /translation="MGCIACAALVLEGEGITLERKESSTIPVEADSICPDRFVGLGQT
                     QIEALPVFYGRRQVRLGDLFTVEGAGADTITVRGDLKAVKKIGYGMSFGRITVMGDAG
                     PHVGAFMKGGEIVVEGSAGDWAGAHMTGGLLVVEGDAGHFTGAAYSGEPKGMRGGTIV
                     VRGSCGREVGGRMRRGLIVVLGDSGEFAGAGMLAGSVFVRGRLGGRPGAGMKRGTIVA
                     LGEAPPMLPTFHYACTYKPVFLRTYLRALRDSGALDEEALAADTFRRYVGDMNTGGRG
                     EVLVRA"
     gene            9990..10955
                     /gene="mch"
                     /locus_tag="VFZ86_15500"
     CDS             9990..10955
                     /gene="mch"
                     /locus_tag="VFZ86_15500"
                     /EC_number="3.5.4.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018759 - methenyltetrahydromethanopterin
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methenyltetrahydromethanopterin cyclohydrolase"
                     /protein_id="HEX5643717.1"
                     /translation="MLSVNERALAISGRLVDAAEAYGVAVQHYENGVTVVDCGIETWG
                     SLEAGRLFSEVCLGGLGSVQLRELHLDGLVTPGVEVGVSHPVVACMASQYAGWAVKAP
                     KTEDTKSYFAMGSGPARALYRGDPIFSSIDYRDPADVAELTLESRTLPPPAVTELIAG
                     KCGVSPDKLHLLMAPTACTVGSVQVAARIVETGIHKMHEVGFDIATVRSGFGSCPLAP
                     VAADDLTAIGRTNDGVLYGGRAWYTVQTDDDQLESVIEQLPSSSSKDYGTTFGELFRR
                     YDHDFYKIDPLLFSPAEVTVTNLTSGRTFRAGGVNPEVLRRSFFA"
     gene            10952..11824
                     /locus_tag="VFZ86_15505"
     CDS             10952..11824
                     /locus_tag="VFZ86_15505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010049338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family alpha-L-glutamate ligase"
                     /protein_id="HEX5643718.1"
                     /translation="MRIAILGQKGGWHTESLLSALARRDVEADCYPVTQLTARVSRRP
                     RLTAGGVPLDACDVVFVRAIPTGSLEQVIFRMDALRMLEHAGVRVVNSAMAIEHGVDK
                     YYTSARLEDAGLPTPRTLVAERFEDAMAAFEELSGDVVVKPLFGSEGRGIVRVTDADT
                     AYRAFRALELGRYVNCIQEFVPHGHEDIRVFVVGERVVGAMLRRGDGWKTNAAQGAKG
                     VPFTPDERVVELSLRATRVVGAEHAGVDILPGDDGEYSVIEVNTIPGWRALRAATGVD
                     AAQCLVDHVIEGRA"
     gene            11821..12714
                     /locus_tag="VFZ86_15510"
     CDS             11821..12714
                     /locus_tag="VFZ86_15510"
                     /inference="COORDINATES: protein motif:HMM:NF013991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase"
                     /protein_id="HEX5643719.1"
                     /translation="MKRLTGDEIAWAAQLACLYEASIEKPGNVSPGVSFSDMRYEDFL
                     TSAVAIGPALRDAGRATVGETIGRAVTDTRRLVAVNTNLGLILLLAPLATAAARVPVG
                     GGDLRAELATVLRALTVQDAVLAYEAIRAAGPAGMGASERHDVREREVTVTLLEAMGE
                     ARRRDAIAREYVTGFRLSFSLGAATILRCWRDGARFSEAVQMAFLTLLAETPDTLIAR
                     KNGVTVAEDVSRHAAEVLAAGGALNDKGRAGIRALTRELRDDTHALNPGTTADLVAAS
                     LFIALTEGGLLGAVTALTDRW"
BASE COUNT         1896 a         4486 c         4585 g         1770 t
ORIGIN      
        1 gccgtgctcg agggcgcgtg cgccgtcctg tgcaccggcg tcgagggcgt ccagctggtc
       61 cccgagtctc tctggcggga gcacccgacc ctccgcgtgc tcgcggacgt caacgcggtg
      121 ccgccgctcg gggtcgaggg cacgaaggcc acctggaacg gcaaggatat cggcgacaag
      181 ctgctcttcg gcgcgctcgg catcggcgac ctcaagatga aggtgcacaa gcgctccgtc
      241 gcccggctct tcgagcgcaa cgacctcgtc ctcgacgccg agggcatcat ggagagcgcc
      301 aaggagctgg ccgggtagtg cgcaccctcg tccttggggt cagcacgcgg gcgatggtcg
      361 agtccgcggt ccgcggcggc cacgacgtcg tcgccgtcga tttcttcggg gaccgcgatc
      421 aggccctcgt cgcggagagc tacgcgctcg aacgcgacct cggcctgccg ccgacggccg
      481 aagggctggg cgaggcggcg cggcgggtcg ctgccgaggc cgtcgtctac ggcgccaacc
      541 tcgagaacca cgccgcggtc gtggcgcggc tggcgggcga gcacgaggtg ctcggcaacc
      601 cgccggaggt gctgcgcgag gtcagggact gggcgctcct gcgccgcgcc tgcgaggagt
      661 ccggcatcgc ccaccccgtc accctgctgc ccggtgagga ggccggcgcg gtcaccggcc
      721 gctggctgcg caagcgtgtc cgcagcggcg gagggcacgg cgtgggtcgc tgggacggga
      781 ggccgctcga cgaggaccac gtgctacaat ccgaggtgga cggcgtgccc gcgtcggtgg
      841 cgttcgtcgc cgacggtcgt gactgccgca tcctcggtct gagcgaacag ctcatcgggc
      901 ttgccgcctt ggggagctcg gggttcgggt ggtgcggcaa catcctgccc ctgggtctct
      961 cccccgcgga gtcggcggcc gcgaacgccg aggtccgccg cgcggcgagc acgctcacga
     1021 ggcgctttgg actgagaggc gtcaacggta tggacgtggt gatcggcagg gaccgcagag
     1081 gcgccgtgcg cccgcacgtc gtcgaggtca atcctcgcta cagcggctcg atggaactgc
     1141 tcgaggacgc cggcggggtc aacgtcttca gcgcccacgt ggacgcctgc gccgggaggc
     1201 tgccggacgc cggcgccgcc cgctccgccg cccccgagcc cgtcggcaag gcgatcgtct
     1261 acgcgcgccg ccgcgtgacg acacccgaca ccggcgcctg gctggcccgc ggcgtgagag
     1321 atgtgccgcg cagccgggag acgatcgccg cgggccaccc gatctgtacc gtcgcggcag
     1381 cgggccgcga ccggcgggag tgcctcgccg gcctcctcgc gcgcgcggcg gaagtgtatg
     1441 cggagagcga agcgagaaag gaggagcgcc gtgagcgacc gactcacctt gatcaccgga
     1501 cgtacgcgta agcaagccat cggcatgcac aagggcaagg cctcggctga gtatctcgag
     1561 gccaccgcgc tcgtggagat gaacccggac gacatgaccc gcctcgacgt gagcgaaggc
     1621 gggcacgtcc tgctgcgcac ggatgatggt gaggtggagc tgacggcccg cgccggcacc
     1681 gtgccggccg gcctcgtctt cgtcccgatg gggacctcgg ccaacaagct gatcggcacc
     1741 gagaccctgg gcacgggcat gccgtgtttc aaggggctcc aggtcgacgt gacgcccctc
     1801 gccggtgagg aggtgtcgcc gtgagcacga cggtcgagaa cgtcgcctgc ccgttctgcg
     1861 ggtgcctgtg cgacgacatc atcgtggaga cggagggcac caagatcctc aaggtcaagc
     1921 tcgcctgctc gagtggtcag gggatcttca tggactacga cccgcggccg cgccggccgc
     1981 tggtccacgg cagcgaggtg gagtggagtg aagccgtcgc cgaggcggcg cgcatcctct
     2041 cggcggccga cagcccgctc atctacgggc tcagctcgtc ggcgagcgag gcgcagcgca
     2101 aggccgtcgc tctcgccgac aagatcggcg ccgtcatcga ctcgacgtcg tccgtctgtc
     2161 acggtcccac ggggctcgcg atgcaggcgg tcggcgagcc cacctgcacg ctgggcgagg
     2221 tacgcgaccg cgccgacctc gtcgtgttct ggggctgcaa cccggacgcc tcgcacctgc
     2281 gtcacttccc gcgcttctcg ctgaccgcga aggggacgct cacgcccaac gggcgcagcg
     2341 accgcacgat gtacgtgatc gacgtgcggc ccacgggcag ctccaagaag gccgaccact
     2401 tcctgcagat cgaggtcggc agcgactacg aggtgctcac caccctgcgc ctgctcgtgc
     2461 gcggcggcga gccgtcgtcc gacgtggtcg gcggcgtgcc ggtggcggag ctcaaggagc
     2521 tggccgagcg catgaagtcg tgccgctacg gcgtcgcctt catgggcatg ggcctcacca
     2581 tggcgccggc gcgacagctc aacgtcgcgg agctgttcac cctggtggcc gagctgaacg
     2641 accacgccaa gttctcggtc atccccatgc gcgggcacgg caacgtcacc ggcgccgacc
     2701 aggtgctctc gtggaccacg ggctacccgt tcgccgtcag tttcgcgcgc ggctatcccc
     2761 agttcatgcc cggggagttc accgccgtcg acatgctcgc gcgcaaggcg gccgacgccg
     2821 cggtgatcat cgcctccgac cccggagcgc acttcccggc cgctgccgca gcgcacctgg
     2881 agcgtatccc cacgatcgtg ttcgaccacg gaccgagtgt ggtcgcggcg aaggccgacg
     2941 ttctcttccc cacggcggcc tacggcgtcg acgccggcgg cacggcgtac cgcatggaca
     3001 acgtgccgat ccctctgacg caggtcatcg agacggatcg tccgaccgac gaggaaatcc
     3061 tcgaccggat catcgaggcg gtggactgat gctgggcatc atcggtggac gggtctacga
     3121 cccgcagaac gacaaggccg gagagatcgg cgacatctgg gtgaaggacg gccgcatcgt
     3181 cccggccgcg gaggtcgacc gcgaaaaggc ggagatcatc gacgccgccg ggctggtggt
     3241 catggccggc ggcgtggaca tccacgcgca catcgtcggc ggcgaggtca acgccggccg
     3301 caagctgcgg ccggaggacc atcgcggcaa ggagcgcgcc cactccggat cgctgcgcgc
     3361 gggcgcgggc ggcacggtgc cgtccaccta cgccaccggg cagctgtact ccgagctcgg
     3421 ctacaccacc gtgatgcagg cgtcgaccgc gccgctgatg gcgcgccaca cgcacgagga
     3481 gctggaggac acgccgaacg tcgacaacgg cgccttcatg gtcatgggca acaaccacat
     3541 cgtgatgcag agcatcgccg acaagcagtt cgaccgcgcc agggactacg tcgcctggct
     3601 gctccaggcg accagaggct acgcgatcaa gctcgtgaac ccgggcgggg tcgagaactg
     3661 gaagtggggt cgtaacgtcg ccgagctcga cgacgaggtg atcggctacg gcgtcacccc
     3721 gcgccagatc atggagacgc tggcgacgat cgccgacgac ctcgggctgc cgcacgccgc
     3781 ccacatccac ggcatcaacc tcgggcggca cacgagcgca cgcaccagca tcgagacgat
     3841 caaggcgctg gacggccacc gggcacacct ctgccacctg cagttcctga gctacggcag
     3901 cggcaagggc cacgtccaca agtcggaggc cgtggccgtc gcggaggccg tcaacgccag
     3961 ccccaagctc accgtcgacg tcggccaggt cgtcttcggg cgggcgacca ccatgacctg
     4021 cgacggcccg ctgcagcaca cgctgcacaa cacggcgggc ggccgctgga tgaacgacga
     4081 cgtcgagggc gagtccggcg gcggcatcgt gccgatcgag tacaagccgg gcaaccccat
     4141 caacgcgacg atgtggctca ccggcctgga gctcttcctt ctcatcgacg acccgtggaa
     4201 ggtgttcctg accaccgacc accccaacgc cggcaccttc agcaactacc cgcagatcat
     4261 ccggctgctg atggaccggg gctatcgcga ggagatgatc gccacgctgc acccgcgcgc
     4321 ccgcaagggg gcgctgctcg ccgagctgga ccgcgagtac tcgctcggcg acatcgccgt
     4381 catcacccgc tccgggcccg cgagggcgct gggcctcaag gacaaggggc acctcggggt
     4441 cggcgccgac gccgacatcg ccgtgtacga ggagcgggac gacaaggagg ccatgttcgc
     4501 ccggccgcgg tacgtgttca aggcgggcga gctcgtggcc cgcgacggcg ccgtcctcaa
     4561 gcaggtggcg ggtcgcacct actcggtagc gcccgactac gaccctagta tagaagacga
     4621 cctgcgcgcc cacttcaagg cggcctacac ggtctcctac gacaactacg ccatgctgtc
     4681 cggcgacggc gcccgcatcg agaccgtcgc ctgcggctga gaggagacgg gatggatgcg
     4741 gaccgcgcct gccgcccgag gcgggacacg caacaggcgc gtcccgccgt cgccgtcgcg
     4801 ctgagccgcg ccggggtgac caacctgcgc cgcatcatcg ccatgacgga cggcggcaag
     4861 gccgagctgt tctacgccgt gctcgacctg tacgccgacc tcagtcccga ccaggccggc
     4921 gtgcacatgt cgcggttcag cgaggtcgtc gaggacctcg ccgagggact cacgcgcaag
     4981 ccgttcgccg acatcgaatc gctggcccgg cgcatggcgg aggacgtcgt cgagacccag
     5041 ggtgcgctgc gcgcggaggt gcacatccgg gcgcagtcgc cgatcaccaa ggtcacgccg
     5101 gtcaccaagc ggcccaccga gcagctgtac acactcgtgg gcatcgccgc cgcgacacgc
     5161 gagcgcacgc gcctgctcgt cggcgtcgag gtcaacggcc tgaccgtgtg cccgtgcgcc
     5221 caggacatgg tccgcgagca ctcggtcgag acgctggtgg aggaggggta cgcggagcag
     5281 gaggccgaac gcatcgtggg gctcctgccg atggcgtcgc acaaccagcg cggcaggggc
     5341 acgctgctga tgggctcgga tcagcgactg cgcgccgagg atctcctgcg catcgcggag
     5401 tcgtccatga gctccgagat ctacgagctg ctcaagcgcc ccgacgagct cgcggtcgtc
     5461 gagaaggcgc accgcaaccc gcgcttcgtc gaggacgtgg tgcgccagat gctcgccagc
     5521 gtctgcaagg agtacgagca cctcgacgac gacacgttcg tgctggcgcg gcaggagaac
     5581 tacgagggca tccacgagca caacgcgttc gcggaacgtt tcggcacact ggcggagatc
     5641 cgcccggaga tcgcggggcg cgccttcgcc cggcaacaca catcgcagag tgagtggctc
     5701 tattcctgag acggcgagga cgcctgacgt ggtcgacacg ctgttcgtca ccggccgact
     5761 ggcggccgat gccctgcgcg cgacgctggc gcggctcgag ctcgacttcg actacgaggt
     5821 ggccgagctg caggggtcgg tggccgcgct catgcgcacg gagtggatcg cccgtcgcct
     5881 gcccgcggac tgcggctgcg ccagggtgat gatccccggc ctgtgccgcg gcgacctcgg
     5941 cctgatcgag gagcgcgtgg gagtgcccgt cgtgcgcggc cccaaggacc tcaaggacct
     6001 gccgcagcac ttcggccagg agcgtgtcct cgagggctac ggcgagtacc acgtgcgcat
     6061 catcgccgag atcgtcgagg cgtggcgtct gcccctcccc gagatcatcg cccaggcgga
     6121 gtacttccgc gcgagcggcg ccgacgtcat cgacctcggc tgcccgccca tggaggagtt
     6181 cccaggcgtc ggcgaggtgg tcctcgccct ccgcgagcgc ggcctcgcgg tgagcatcga
     6241 cacgttccac accgcgacca tccgcgaggc cgacgaggcc ggcgtcgacc tgctgctgag
     6301 cgtgaacggg cagaacctgg acatcgtgcc cgacctccgc tgcaaggtgg tcgtgatccc
     6361 cgacttcggc gaagggctgg actcgctcga gcgcaacgtg gaccgcgtcg ccgagctcgg
     6421 cgcggcgtac gtcatcgacc cgatcctcga ccccatcagc ttcggattca cctcctcgat
     6481 cgagcgctac tgcgaggtcc gccgccgcca ccccgaggcg gaggtgctca tgggcctcgg
     6541 caacctgacc gagctcaccg acgccgacag cgtgggtgtc aacgcggtga tggcgggggt
     6601 catcgaggag ctccacatcg actacgtgct caccacggag gtgatcagct gggcgcgcgg
     6661 cgccgtgcgc gagctcgatc acgcgcgcaa gctgatgcac tacgccgtcg gcaaccgcac
     6721 gctgcccaag aatctcgacg acgcactggt agcgctcaag gatcccccct tcgagagcta
     6781 ctccgaggcg gagttgcgcg acatgcagca tcaggtgcgc gaccgcaact tccgcatctt
     6841 cgccgacggc gaggcgatca ccgtcttcaa ccgggcgctc ttcctgcgag gcaccgaccc
     6901 tcgcgagctg ttcgcggcgc tgggtgtgga ggaggccagc cacgccttct atctcggccg
     6961 ggagctggag cgtgcgtccc tcgccgtgcg tctcggcaag aagtacgtgc aggagtcgcc
     7021 gctgcgctgg ggctacctgg gcgcggacga cgacgccgat ccggcggagg agccggcgtg
     7081 acgcgcgtcg tcggcatcga cccggggacc gtgagcctgg acctctgcgg gcgcgacggc
     7141 gacgccgtct tcctcgacga gagcttcccg accgccacga tcggcgccga cccggggatg
     7201 ctcgtcgagc tcctgcaggg cgcccggccg ctggacctcg tcgtcggccc gtccggctac
     7261 ggcctgccct ggaccgacgt ccgcgacgtc ggcgccgggg agctcgcgct gctcttcctc
     7321 gccgatgccg ccgacggccc gcgcgggacc atcctgggcg gcatgggacg cctcgtggag
     7381 gcgctccggg tgagcggcct gccggtgtgc ttctcaccct cggtcgctca cctgcccacc
     7441 gtccccgctc accgcaaggt caaccgcatc gacatgggga ccgccgacaa gctctgcgcg
     7501 gtggcgctcg gcgtgtggga ccaggcgcgc cggctcggcc tgccctgctc cgccacctcg
     7561 ttcgtcttcg tcgaggccgg cggcgccttc acggcggtca tcggggtcga cgggggcgcg
     7621 gtggtggacg gcgccggcgg caccagcggc gctcccggct ttcgcgccat gggcgccatg
     7681 gacggtgagc tcgcctacct gctgcgcgac ttctccaagg acgtcctggc cagcggcggc
     7741 gccgcctggg tctgcggcga tccggactgc acgccgggcg acctcgtcag ggccgccgcg
     7801 gcgggcggac cggcggccgt cgccctcgac gccctgctcg agggcatcga gaagcaggtg
     7861 gcggcgcagg tgaccgtcct ggggacgccg cgtgagatcc tcctctcggg gcgcctcagc
     7921 cgcgtccccg agatccggcg gctcctggcc gaacggctct cccggttcgc ccccgtctgc
     7981 gacgtcggcg gcttcgccgc cgtcgccagc gaggcggcac agggcgccgc gctgctcggg
     8041 cagggactgg ccgggcaggc cggcctgcgg gacctcgtcg aggcgctgca ggtgcgctcc
     8101 gccgccggct ccgtgctgga ccacctctac gtcagcgaga ccgagtctct ccggcgccgc
     8161 cacccgtcgg ccgggcgggc gccggcaccc ttctgggaaa ggagctagcc tctcatgcat
     8221 ctcaacggag tggagataga ggacacgttc gcggaagcct tcggcatgtg gggctcacgc
     8281 ctcatcgtga ccgcggagac gctggagtgg gcgcgcacgg cggccgcggc ggcgacgggg
     8341 ttcgccacgt cgatcatcgg ctgcggctgc gaggcgggcg tcaacgagct cggcccggac
     8401 gaggagaccc cggacggccg gcccggcatc gcagtgctcg tgttcgcgac ctcgcgctcg
     8461 aacatggagt cggtgctgct ggccagggtg ggacaggcca tcatgacgtc ggcgacctcg
     8521 gcctgctacg acgggctggg cggcgacgag aggctcaagg tgggcggcaa gctgcgctac
     8581 ttcggcgacg gcttccagcg cagcaagctg ctcgacggtc gccgcctgtg gcgcgtcccg
     8641 gtcatggacg gggagttcgt cgtcgaggag agcttcggcg tggccaaggg cgtcggcggc
     8701 ggcaacttct tcatgatggg ggaggacctg gaggccgcgc tcaccgccgc ggaagcggcg
     8761 gtcgcggcga tgcgctccgt gcccggtgcg atcatgccgt ttcccggagg agtggtgcgc
     8821 tccggcagca aggtctcgtc ccgctacaag ggccagaacg tgtcgagcaa cacgccgtac
     8881 tgccccacgc tgcgcgccca ggagcagacg gcgctgcgcg agggcgagaa cgccgtcctc
     8941 gagatcgtga tcgacgggct ggacgagcag gcggtgcgcg aggccatgcg ggtgggcatc
     9001 acggcggcct gcctccccgg cgtgacgcgc atctcggccg gcaactacgg cggctccctg
     9061 gggcagttcc acttcgccct gcgggagctg gtgaccgcgt gatcgccgcg tggagggacg
     9121 ctcccgcgat gaggaggagg cagcagccat gggatgcata gcttgtgcgg cgctcgtgct
     9181 cgagggagag ggcatcacgc tggagcgcaa ggagtccagc acgatccccg tcgaggccga
     9241 cagcatctgc ccggatcgct tcgtcgggct cgggcagacg cagatcgagg cgttgccggt
     9301 gttctacggg cgccggcagg tgagactcgg cgacctcttc acggtcgaag gcgccggcgc
     9361 cgacaccatc accgtgcgcg gcgacctcaa ggcggtcaag aagatcggct acggcatgag
     9421 cttcggccgc atcaccgtga tgggcgacgc cggcccccac gtcggtgcgt tcatgaaggg
     9481 cggcgagatc gtcgtggagg gcagcgccgg cgactgggcc ggcgcccaca tgacgggcgg
     9541 tctcctggtc gtcgagggag acgccggcca cttcaccggc gccgcctact cgggcgagcc
     9601 caagggcatg cgcggcggga ccatcgtcgt gcgcggctcg tgcggccgtg aggtcggcgg
     9661 ccgcatgcgc cgcgggctca tcgtcgtcct cggcgactcc ggcgagttcg ccggcgccgg
     9721 catgctggcc ggctcggtct tcgtcagagg ccgcctcggc ggccgcccgg gagccgggat
     9781 gaagcgcggg accatcgtcg ccctcggcga ggcgccgccg atgctgccca cgttccacta
     9841 cgcctgcacc tacaagcccg tgttcctgcg gacgtacctg cgcgccctgc gggactccgg
     9901 cgcgctggat gaagaggcgc tcgcagccga caccttccgg cgctacgtcg gcgacatgaa
     9961 cacgggcggc cgaggggagg tcctggtccg tgcttagcgt gaatgagcgg gcgctcgcca
    10021 tctccgggcg actcgtggac gccgccgaag cgtacggcgt ggccgtccag cactacgaga
    10081 acggcgtcac cgtcgtcgac tgcggcatcg agacgtgggg ctcgctcgag gccgggcgcc
    10141 tcttctccga agtctgtctg ggcggcctcg gcagcgtcca gctgcgagag cttcacctgg
    10201 acggcctcgt gacgcccggc gtcgaggtgg gcgtgagcca cccggtcgtg gcctgcatgg
    10261 cctcccagta cgcagggtgg gcggtgaagg cgcccaagac cgaggacacg aagtcgtact
    10321 tcgcgatggg ctcgggaccg gcgcgggcgc tgtaccgggg cgatcccatc ttctccagca
    10381 tcgactaccg cgacccggcg gacgtggccg agctcacgct ggagtcgagg acgctgccgc
    10441 cgccggccgt gaccgagctg atcgccggga agtgcggcgt gagcccggac aagctccacc
    10501 tgctgatggc gcccaccgcc tgcaccgtcg gctcggtgca ggtggcggcg cgcatcgtcg
    10561 agaccgggat ccacaagatg cacgaggtcg gcttcgacat cgccacggtg cgttcggggt
    10621 tcggctcctg cccgctggcg ccggtcgccg ccgacgacct caccgccatc ggtcgcacga
    10681 acgacggcgt gctctacggc gggcgcgcct ggtacacggt gcagaccgac gacgaccagc
    10741 tggagagcgt catcgagcag cttccgtcga gctcgtcgaa ggactacggc acgaccttcg
    10801 gcgaactgtt ccgccgctac gaccacgact tctacaagat cgacccgctc ctgttcagtc
    10861 cggcggaggt gacggtcacc aacctgacca gcgggcgcac gttccgcgcc ggcggggtga
    10921 acccggaggt cctccggcga tcgttcttcg cgtgaggatc gccatcctgg ggcagaaggg
    10981 ggggtggcac acggagagcc tcctctcggc gctggcgcgc cgggacgtcg aggcggactg
    11041 ctacccggtc acgcagctga ccgcgagggt cagccgccgc ccacgcctca cggccggcgg
    11101 cgtcccgctc gatgcgtgcg acgtcgtgtt cgtgcgcgcc atcccgaccg gctccctgga
    11161 gcaggtgatc ttccgcatgg acgccctgcg catgctcgag cacgccggcg tgagggtggt
    11221 caactcggcg atggcgatcg agcacggcgt ggacaagtac tacacctcgg cgcggctcga
    11281 ggacgccggg ctgccgacgc cgcgcacgct ggtcgccgaa cgcttcgagg atgccatggc
    11341 cgccttcgag gagctgagcg gcgacgtcgt cgtcaagccc ctgttcggct ccgagggccg
    11401 cggcatcgtc cgggtcacgg acgccgacac cgcgtatcgc gccttccgcg ctctcgagct
    11461 gggccgctat gtcaactgta tccaagagtt cgtgcctcac ggccacgagg acatccgcgt
    11521 cttcgtcgtg ggggagcgcg tcgtcggggc gatgctgcgg cgcggcgacg gatggaagac
    11581 caacgccgcg cagggcgcca agggcgtgcc gttcacgccg gacgagcggg tggtcgagct
    11641 gagcctgcgc gcgacgcgcg tggtgggcgc agagcacgcc ggcgtcgaca tcctgccggg
    11701 cgacgacggc gagtacagcg tcatcgaggt gaacaccatc ccggggtggc gcgcgctgcg
    11761 cgcggccacg ggcgtcgacg ccgcgcagtg tctcgtcgac cacgtgatcg agggccgggc
    11821 gtgaagcgcc tgacgggcga tgagatcgcc tgggcggcgc agctggcctg cctgtacgag
    11881 gcgagcatcg agaagccggg caacgtatcg ccgggcgtct ccttctcgga catgcgctac
    11941 gaggacttcc tgacgagcgc ggtggccatc ggtcccgcgc tccgcgacgc ggggcgcgcc
    12001 acggtaggcg agacgatcgg cagagccgtc acggacacgc gccgtctcgt cgccgtcaac
    12061 accaatctgg ggctgatcct gctgctggcc cccttggcga cggccgcggc gcgcgtgccc
    12121 gtcggcggcg gcgacctgcg tgcggagctt gccacggtgc tgcgcgccct gacggtccag
    12181 gatgccgtcc tggcctacga ggccatccgc gccgccggcc ccgcggggat gggcgcgagc
    12241 gagcgccacg acgtccgcga gcgcgaggtc accgtgacgc tgctcgaggc gatgggcgag
    12301 gcacgccggc gcgacgccat cgcccgcgag tacgtcaccg gcttccgcct ctccttctcg
    12361 ctcggtgcgg cgacgatcct ccgctgctgg cgcgacggcg cccggttctc cgaggcggtc
    12421 cagatggcct tcctcaccct ccttgcggag accccggaca cgctcatcgc gcgcaagaac
    12481 ggcgtcaccg ttgcggagga cgtgtcacga cacgcggccg aggtgctggc cgccggcggt
    12541 gcgctgaacg acaagggacg cgccgggatc cgggccctga cgcgcgagct ccgggacgat
    12601 actcacgcgc tcaaccccgg cacgaccgcc gacctcgtcg cagcgtcgct gttcatcgcc
    12661 ctgacggagg gcggcctgct cggcgcggtg accgcgctca cggaccgctg gtgaacgggt
    12721 ggccgtccga gcgcaca
//
LOCUS       DASUMX010000326         2131 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_2142744, whole genome shotgun sequence.
ACCESSION   DASUMX010000326 DASUMX010000000
VERSION     DASUMX010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2142744"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2028
                     /gene="pheT"
                     /locus_tag="VFZ86_15515"
     CDS             <1..2028
                     /gene="pheT"
                     /locus_tag="VFZ86_15515"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX5643720.1"
                     /translation="LELTSNRPDCFSIYGIAREVAAAARRELMPTPIAAPAAFGGAPA
                     SAAIQVEIADPDLCPRYGATVIRGVRIGESPAWLKARLTHAGMRPINNVVDVTNYVML
                     GWGQPLHAFDAGKIRGGTLIARRAQAGEKIVTLDGVERTLDDQMLVIADVERALVIAG
                     VFGSQDAEIDEHTTDLVLEAANFAGPSILRTEMHTGIRSEASNRFEKGIDANLVPGGL
                     DFADRLFAELCGGTVAPGMVDAGGVLPAPEPLRYRPARADGLLGYAVPAGEQAGILRR
                     LECAVDEAGVASPGPGSPASEPGAGETWTVTPPTFRPDLIREVDLIEEVGRIAGYGMA
                     PETLPRHTTAGGLTKPQQVRRAVRRALAGCGLDEVITYSFIAPDALAPLGLPDGDVRL
                     DPVRLSNPMSVEQSVMRTMLLPGLLKTVRDNVDRLNDPPNLFEIGKVYLWDEPVPAPE
                     HAAEPGAVLPHEPEALGIVLSGPLQAENWTGAGRATDFYALKGAVDAALAAVGLRGEY
                     GPLGEAAAHFPFLHPGKAALVSVPGAGGVGALGQLRPDVAAACGVDDLAVYYASLTMD
                     RISPAALATPAFADLGAYPPASQDLAVVVGREVPAAAVVEQARRAGGKLVRSVRVFDV
                     YEGDQVAADKRSLALRVVMRAPDRTLNEKDIAGVRAKILKALEREFQAELR"
BASE COUNT          273 a          828 c          759 g          271 t
ORIGIN      
        1 ctcgagctga cgtcgaacag gcccgattgc ttcagcatct acggcatcgc ccgcgaggtc
       61 gcggcggcgg cccggcgcga gctgatgccg acgcccatcg ctgccccggc ggcgttcgga
      121 ggcgccccgg cgtccgccgc catccaggtc gagatcgcgg accccgatct gtgcccgcgg
      181 tacggcgcca ccgtcatccg cggcgtgcgc atcggcgagt cgccggcgtg gctcaaggcg
      241 cgtctcacgc acgcgggcat gcgccccatc aacaacgtgg tcgacgtgac caactacgtg
      301 atgctcggct ggggccagcc gctgcacgcg ttcgacgccg gcaagatccg cggcggcacg
      361 ctcatcgcgc ggcgggcgca ggccggcgag aagatcgtca ccctggacgg cgtcgagcgg
      421 acgctggacg accagatgct ggtcatcgcc gacgtcgagc gcgccctggt catcgccggc
      481 gtgttcggca gccaggacgc cgagatcgac gagcacacca ccgacctcgt tctcgaggcg
      541 gccaacttcg ccggcccgag catcctgcgc accgagatgc acaccggcat ccgcagcgag
      601 gccagcaacc gcttcgagaa gggcatcgac gccaacctgg tgcccggcgg cctcgacttc
      661 gccgacaggc tgttcgccga gctctgcggc ggcacggtcg cgccgggcat ggtcgacgcc
      721 ggcggcgtgc tgccggcgcc cgagccgctg cgctaccgcc cggccagggc cgacgggctg
      781 ctcggctacg cggtcccggc gggcgagcag gccggcatcc tgcgccggct ggagtgcgcg
      841 gtggacgagg ccggcgtggc gtcgcccggc cccggctcgc ctgcgtccga gcccggcgcc
      901 ggtgagacct ggaccgtcac cccgccgacg ttccggcccg acctcatccg cgaggtggac
      961 ctcatcgagg aggtcgggcg catcgccggc tacggcatgg cgcccgagac cctgccgcgg
     1021 cacaccaccg ccggcgggct gaccaagccg cagcaggtgc ggcgcgccgt tcggcgcgcc
     1081 ctggccggct gcggcctcga cgaggtcatc acctacagct tcatcgcccc cgacgccctc
     1141 gcgccgctgg gcctcccgga cggcgacgtg cgcctcgatc ccgtgcggct ctccaacccg
     1201 atgagcgtgg agcagtcggt gatgcgcacc atgctgctgc ccggcctgct caagaccgtg
     1261 cgcgacaacg tcgaccggct caacgacccg ccgaacctgt tcgagatcgg caaggtctac
     1321 ctctgggacg agccggtccc ggcgccggag cacgccgccg agcccggcgc cgtcctgccg
     1381 cacgagccgg aggcgctcgg catcgtcctc tccggtccgc tacaggctga gaactggacc
     1441 ggcgccggcc gcgccactga cttctacgcg ctcaagggcg ccgtggacgc cgcgctggcc
     1501 gccgtcggcc tgcgcggcga gtacgggccg ctcggcgagg ccgcggcgca cttccccttc
     1561 ctgcatccgg gcaaagccgc gctcgtgagc gtgcccggcg cgggcggggt cggggcgctc
     1621 ggccagctgc ggcccgacgt ggccgccgcc tgcggcgtcg acgacctggc cgtgtactac
     1681 gcctcgctga ccatggaccg gatctcgccg gccgcgctcg cgacacccgc cttcgcggac
     1741 ctcggcgcct acccgccggc ctcgcaggac ctcgccgtgg tcgtcggccg cgaggtcccg
     1801 gcggcggccg tcgtcgagca ggcccgccgc gccggcggca agctcgtgcg cagcgtccgc
     1861 gtcttcgacg tctacgaggg agaccaggtg gccgcggaca agcgctcgct ggctctgcgc
     1921 gtcgtcatgc gcgcaccgga ccgcacgctg aacgagaagg acatcgccgg cgtgcgcgcc
     1981 aagatcctca aggcgctcga gcgggagttc caggccgagc tgcgctgaca cggcgccgcc
     2041 cgctcgtttg ccgggcgcgt cacggagtcg gtgggccggc gtcggagctg ccggacgacc
     2101 tcgttcgccg aaccctgccg ccggttccgt c
//
LOCUS       DASUMX010000327         5317 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_130666, whole genome shotgun sequence.
ACCESSION   DASUMX010000327 DASUMX010000000
VERSION     DASUMX010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 5317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5317
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130666"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..635)
                     /locus_tag="VFZ86_15520"
     CDS             complement(3..635)
                     /locus_tag="VFZ86_15520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HEX5643721.1"
                     /translation="MADIYAEAAESIVSADRAAAEEVAQRALDSGIAPADIMQNGFVK
                     GIQEVGDLFESGEVFLPELMMSADAMAGATEVTNAALEGEAAEAKGVMVIGTVQGDVH
                     DIGKAIVIAYLKANGYEVTDLGRDVSADSFIDRAKELGADVIGMSALLTTTMEEQKKV
                     IKRVKDEGLPFKTVVGGAPCTQRWADQIGADAYAEDANDGVKKIDALLGK"
     gene            930..1808
                     /locus_tag="VFZ86_15525"
     CDS             930..1808
                     /locus_tag="VFZ86_15525"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX5643722.1"
                     /translation="MRYHGAVIRPPSEADSLIVQVTYGCSNNTCDFCGTYLEKPFAVR
                     PFDEVVEDVTGLPVSVRQRVRRVFLADGDAMALSTRKLDAILDLLHRALPNLERVSSY
                     ANARNLLGKSVEELAALRERGIELLYLGLESGDEQTLADIHKGMTVAEQIEGCRRASD
                     AGVQLSVTAILGLAGRERSLVHARATGEALSAIDPEYIGILTLMLPPGTAMERKVAGG
                     QVVLPDSLGILRELREIVAHTDVTGSLFRCNHASNYLPIGGRLPEDKAAILATLDKVL
                     DAPAATQLRPESWRLL"
     gene            complement(1819..2148)
                     /gene="pylSn"
                     /locus_tag="VFZ86_15530"
     CDS             complement(1819..2148)
                     /gene="pylSn"
                     /locus_tag="VFZ86_15530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014184090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043767 - pyrrolysyl-tRNA synthetase
                     activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrrolysine--tRNA(Pyl) ligase small subunit"
                     /protein_id="HEX5643723.1"
                     /translation="MPTDPTKARYYRKQVPLFRLVEKMKLWPSRRGILHGIKQFEERG
                     SYAVVVTHCNKHMVVHDSKTSRAARWLRNKWYAEACPQCRIPEWKLQKYASTHFKRGY
                     GSHLHDL"
     gene            2255..3184
                     /locus_tag="VFZ86_15535"
     CDS             2255..3184
                     /locus_tag="VFZ86_15535"
                     /inference="COORDINATES: protein motif:HMM:NF022114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HEX5643724.1"
                     /translation="MTRRAVAGFARLLPLLLIAAAVVIVVGAALLGACGDSSGGASPE
                     PAATVTVTASPSGAPSAAASPSPSTAPAQTTVSLYFLRDEKLGVAERRVKHTTMPATA
                     AMKALLAGPAATEQDAGLGTAIPAGTRLLGLSIAEGTARVDLSSEFAAGGGSLSMTAR
                     VAQVVYTLTRFPTVRAVEVLVDGEPVEALGGEGIVLDGAQRRADWRGLEPNIFVESPG
                     VGAVLSSPFLLSGTAMVFEGSFQARLGDSSGRRIVNVIVQASAGAPGRGRFSQEVPFS
                     TSAGRGTLVVFDQSMEDGSRQDEVRIPVTFATD"
     gene            complement(3219..3677)
                     /locus_tag="VFZ86_15540"
     CDS             complement(3219..3677)
                     /locus_tag="VFZ86_15540"
                     /inference="COORDINATES: protein motif:HMM:NF039409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6328 family protein"
                     /protein_id="HEX5643725.1"
                     /translation="MAEDRKERVDRELIELLNEIRVALPGAQVLFAFLLTLPFTQRFG
                     SLGSLDRDAYFAAFICTALGTSMLMAPTMHHRLRFREHDKERMLLGFNKLVLVGSVFL
                     AAAIALSVYVVTGMLFEATWASVTGAAIAAWLVVLWYVLPLLQRRGRELD"
     gene            complement(3939..4745)
                     /gene="pylD"
                     /locus_tag="VFZ86_15545"
     CDS             complement(3939..4745)
                     /gene="pylD"
                     /locus_tag="VFZ86_15545"
                     /inference="COORDINATES: protein motif:HMM:TIGR03911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methylornithyl-N6-L-lysine dehydrogenase PylD"
                     /protein_id="HEX5643726.1"
                     /translation="MTRLTEADVTTLTRDLEAFEARLLEATGLDLRDLALRTAVDGDR
                     CVRLHGARIAAVPMSSGDGVIPGFSACVVATLLHLGCDAWMTTQPDVRGIQAAAAAGA
                     SVLFIADDHRFIALSLANGSSIDDDPATADGYVTALEAAAGGLDGREVLVLGLGPVGR
                     AAVRRLGKRGANVLVAEPDGERIVVARDVGLVFEAVELAAGLDRCALVFDASPAAGII
                     DAAAVSPETIAAVPGMPSAFTPAAQEALGVRHIHEPLAIGVAVMAVRALL"
     gene            complement(4742..>5317)
                     /locus_tag="VFZ86_15550"
     CDS             complement(4742..>5317)
                     /locus_tag="VFZ86_15550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643727.1"
                     /translation="PGGACDWEAAVAPGVLAALDDAGVRIAEGLGLNGLMDVEVMVSG
                     TRPKLLEIDARLPSQTPTAVYWSSGLNIVELLARTVLEGAPPVVDRRAVRACVYQHVR
                     AARGMLEVLGEHVMGSARPLRLVPGFFGADEALTDYAPGRAEWAATLIVAAESARAAR
                     AQAAAVVAELARYEGLRAAPDDDPLGEGGPR"
BASE COUNT          747 a         1944 c         1854 g          772 t
ORIGIN      
        1 gcttacttcc ccagcagcgc gtcgatcttc ttgacgccgt cgttggcgtc ctcggcgtag
       61 gcgtcggcgc cgatctggtc ggcccagcgc tgcgtgcagg gggcgccgcc gacgacggtc
      121 ttgaagggca ggccctcgtc cttgacgcgc ttgatcacct tcttctgctc ctccatggtg
      181 gtggtgagca gggcgctcat gccgatgacg tcggcgccga gttccttggc gcggtcgatg
      241 aagctgtcgg cggagacgtc gcggccgagg tcggtgacct cgtagccgtt ggccttcagg
      301 taggcgatca cgatcgcctt gccgatgtcg tgcacgtcgc cctggacggt gccgatgacc
      361 atgacgccct tggcctcggc ggcctcgccc tcgagggcgg cgttggtgac ctcggtggcg
      421 ccggccatgg cgtccgcgct catcatcagc tcgggcagga agacctcgcc tgactcgaac
      481 aggtcgccga cctcctggat gcccttgacg aagccgttct gcatgatgtc ggccggggcg
      541 atgccggagt cgagggcgcg ctgcgcgacc tcctcggctg ccgccctgtc ggcgctgacg
      601 atgctctcgg ccgcttctgc gtagatgtct gccatgaccc tggactcctc ccgactttgc
      661 tcacgactgt ttccgcgcaa ctaggtatgg cgggtgccga gatcgggcga tgacaccgca
      721 agccgggaag cgatcccgcc ggacccgatc cggcttgaca ctccccgacg ccctccctcg
      781 acacgtgcgg gcagtatatc agccgttggg aagcacggcc acacactctc actcacaaac
      841 gcacaaaaac gacgagcgat cgccgcgcgg ccggcggcgg gaaggcgccg gcgggcgcgg
      901 ccccgtggag ccgtgctagc atcggccgcg tgaggtacca cggcgccgtc atccgccctc
      961 ccagcgaagc agacagcctg atcgtccagg tcacctacgg ctgctccaac aacacctgcg
     1021 acttctgcgg cacctacctg gagaagccgt tcgcggtgcg gccgttcgac gaggtcgtgg
     1081 aggacgtcac cggcctgccg gtgagcgtgc gccagagggt gcggcgcgtg ttcctcgccg
     1141 acggcgacgc gatggccctg agcacgcgca agctcgacgc gatcctcgac ctgctgcatc
     1201 gcgccctgcc caacctggag cgcgtgagca gctacgccaa cgcgcgcaac ctgctgggca
     1261 aatcggtgga ggaactcgct gcgctgcgcg agcgtggcat cgagctcctc tatctcggcc
     1321 tcgagtccgg cgacgagcag acgctggcgg acatccacaa gggcatgacc gtggcggagc
     1381 agatcgaggg gtgccggcgc gcctcggacg ccggcgtgca gctctcggtg accgccatcc
     1441 tggggctcgc cgggcgcgag cggtcgctcg tgcacgcccg ggccaccggc gaggcgctct
     1501 ccgccatcga ccccgagtac atcggcatcc tcacgctcat gctgcccccc ggtaccgcga
     1561 tggagcgcaa ggtcgccggt ggccaggtcg tgctgcccga ctccctcggg atcctgcgcg
     1621 aactgcgcga gatcgtcgcc cacaccgacg tcaccggctc gctcttccgc tgcaaccacg
     1681 cctccaacta cctgccgatc gggggccgcc tgccagagga caaggcggcc atcctggcca
     1741 ccctggacaa ggtgctggac gcgccggccg cgacgcaact gcggccggag agctggcggc
     1801 tgctctagtc gcgctcgctc agaggtcgtg caggtgcgac ccgtacccgc gcttgaagtg
     1861 cgtggacgcg tacttctgca gcttccactc ggggatgcgg cactgcgggc acgcctcggc
     1921 gtaccacttg ttgcgcagcc agcgtgcggc cctgctcgtc ttggagtcgt ggacgaccat
     1981 gtgcttgttg cagtgcgtca ccacgactgc gtaggagccg cgctcctcga actgcttgat
     2041 cccgtgcagg atgccgcgcc gcgagggcca cagcttcatc ttctcgacga gacggaagag
     2101 cgggacctgc ttgcggtagt aacgcgcttt ggtcgggtcg gtcggcatcg ggtggccagt
     2161 ctagcacctc gacgcggaac ggtcggctgg ggcaggagcg gagagccgcg gcgtcgaagg
     2221 ttcggccccg atccaccgcg acgcgaggtg ccccatgacc cgccgtgccg ttgccggctt
     2281 cgcccgtctc ctgccactgc tgctgatcgc cgcggcggtg gtgatcgtcg tgggcgccgc
     2341 tctgctcggc gcctgcggcg actcctccgg cggcgcgtcc ccggaaccgg cggccacggt
     2401 cacggtcacg gccagcccgt ccggcgcgcc ttccgcagcc gcgtcgccgt ccccgtcgac
     2461 agcccccgcc cagaccacgg tcagcctgta cttcctgcgt gacgagaagc tcggcgtggc
     2521 cgagcgccgc gtgaagcaca ccacgatgcc ggccacggcg gcgatgaagg ccctgctcgc
     2581 cggccctgcc gcgaccgaac aggacgccgg gctcgggacc gccatccccg caggcacgcg
     2641 cctgctcggc ctgtcgatcg ccgaggggac ggcgcgcgtc gacctctcga gcgagttcgc
     2701 cgccggcggc ggctcgctct cgatgacggc gcgggtcgcg caggtcgtct acacgctgac
     2761 gcggttcccg accgtgcgcg cggtcgaggt cctcgtcgac ggcgagcccg tcgaggccct
     2821 gggcggcgag ggcatcgtcc tcgacggcgc ccagcgccgc gccgactggc gcggcctcga
     2881 gcccaacatc ttcgtcgagt cgccgggcgt cggcgcagtc ctctccagcc ccttcctgct
     2941 ttcgggtacc gccatggtgt tcgagggatc gttccaggcg cggctcggcg actcgtccgg
     3001 ccgccgcatc gtcaacgtca tcgtgcaggc cagcgcgggc gcgcccgggc gcggacgatt
     3061 ctcccaggaa gtccccttct cgacctccgc cgggcgcggc accctcgtcg tgttcgacca
     3121 gtccatggag gacggctcgc gccaggacga ggtcaggatc cccgtcacct tcgcgaccga
     3181 ctgacgcgtt ccgctcggtc ccgccagcgc tacgtcgatc agtcgagttc gcgcccccgc
     3241 cgctgcagca gcgggaggac gtaccacagc accaccagcc acgccgcgat cgccgcgccg
     3301 gtcaccgagg cccaggtggc ctcgaagagc atgccggtca ccacgtagac cgacagggcg
     3361 atggcggcgg cgaggaacac cgaccccacg aggaccagct tgttgaagcc gaggagcatg
     3421 cgttccttgt cgtgctcgcg gaagcgcagc cggtggtgca tcgtgggcgc catcagcatc
     3481 gacgtgccca gggccgtaca gatgaacgcc gcgaagtacg cgtcccggtc cagtgagcca
     3541 aggctcccga accgctgcgt gaacggcagt gtgagcagga aggcgaagag gacctgcgcc
     3601 ccgggcaggg cgacccggat ctcgttgagg agctcgatga gctcccggtc gactctctcc
     3661 ttgcgatcct cggccacggc gcgctccggc ggatgacgac gacaagcgtc atgttccccg
     3721 gatcgatcga tgcgcgcgtc gcgccgtaag aggtgcggag aggacgcgcc gtgccgcgga
     3781 gagagaggct ggcggggagt gcgggagtca cacccgccta cgtgggttta gagcccacgc
     3841 tgatcctttc gatcgacccc ccgcgccctg cgattgtaca ccggcgcgac cccagctcgc
     3901 gtcgccggcc cctgggccgc gccaggacgg cgcgatcctc agagcagagc gcgcaccgcc
     3961 atgaccgcga cgccgatggc cagcggctcg tggatgtggc gcaccccgag ggcctcctgc
     4021 gccgccgggg tgaacgcgga gggcatgccg ggcaccgcgg cgatggtctc ggggctgacg
     4081 gcggcggcgt cgatgatccc ggccgccggc gaggcgtcga agaccagggc gcagcggtcc
     4141 aggcccgccg caagctccac ggcctcgaag acgaggccga cgtcgcgggc cacgacgatg
     4201 cgctcgccgt cgggctcggc caccaggacg ttcgcgccgc gcttgccgag gcggcggacg
     4261 gcggcgcgcc cgacggggcc caggccgagg accaggacct cgcggccgtc gaggcctccc
     4321 gcagccgcct cgagcgccgt gacgtacccg tcggcggtgg cgggatcgtc gtcgatgctg
     4381 ctgccgttgg ccaggctgag ggcgatgaag cggtggtcgt cggcgatgaa gaggacgctc
     4441 gcccccgccg cggcggccgc ctggatgcct ctcacgtcgg gctgcgtggt catccacgcg
     4501 tcgcagccca ggtgcagcag cgtcgccacc acgcaggcgg agaagccggg gatcaccccg
     4561 tccccggaag acatggggac ggcggcgatg cgcgccccgt gcagccgcac gcagcggtcg
     4621 ccgtcgacgg cggtccgcag cgcgaggtcg cgcaggtcga gcccggtggc ctcgaggaga
     4681 cgcgcctcga aggcctcgag gtctctcgtc agtgtcgtca cgtcggcttc ggtcagtcgt
     4741 gtcatcgcgg gcctccctcg cccagaggat cgtcgtcagg tgccgcccgc aggccctcgt
     4801 accgcgccag ctccgcgacc acggcggcgg cctgcgcgcg cgctgcgcgc gcgctctccg
     4861 cggccacgat cagcgtcgcg gcccactcgg cgcggccagg cgcatagtcc gtgagcgcct
     4921 cgtccgcgcc gaagaacccg ggcacgagac gcagcggacg ggcactcccc atcacgtgct
     4981 cgccgaggac ttccagcata cctcgcgcgg cgcgcacgtg ctggtagacg caggcgcgca
     5041 cagccctgcg gtccaccacc ggcggagccc cctcgaggac cgtccgcgcg agcagttcca
     5101 cgatgttcag cccgcttgac cagtagaccg ccgtcggcgt ctggctgggc aggcgggcgt
     5161 cgatctccag cagcttcggt cgcgtgccgc tcaccatgac ctcgacgtcc atgagcccgt
     5221 tcagcccgag gccctcggcg atgcgcacgc cggcgtcgtc gagagcggcg agcacgcccg
     5281 gcgcgacggc ggcctcccag tcacaggcgc cgcctgg
//
LOCUS       DASUMX010000328         7758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1855105, whole genome shotgun sequence.
ACCESSION   DASUMX010000328 DASUMX010000000
VERSION     DASUMX010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 7758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7758
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1855105"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="VFZ86_15555"
     CDS             <1..299
                     /locus_tag="VFZ86_15555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX5643728.1"
                     /translation="FTRNELTRFLEANRVETRNLFSGNLLRHPAFEDIERRVVGDLAA
                     TDAVMERTFFVGVYPGIDEPRLQYMIDVFGRFMDGERAVGVGADGVPTPGPEDR"
     gene            309..1001
                     /locus_tag="VFZ86_15560"
     CDS             309..1001
                     /locus_tag="VFZ86_15560"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5643729.1"
                     /translation="MAVVRKIACRVAGVTGHGERVYTVELDPVAPAPRFRPGQFLHLA
                     LDDYEPGGFWPESRVFSIASAPRARDRLAITYAVKGRFTARMEDELAEGREVWVKLPY
                     GEFVIDQSREAVLFAGGTGVTAFTAFLQSLDAGRAPRVLLFYGARTPDLFVYGSLVEA
                     CAREVPSLSSILVAETSGGVLSVARAWPAIKELDEPIFYLSGPPAMLTALTEQLLERG
                     VCAATIRTDAWE"
     gene            1013..2851
                     /locus_tag="VFZ86_15565"
     CDS             1013..2851
                     /locus_tag="VFZ86_15565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643730.1"
                     /translation="MVEAPPEGPGALPASGGRNGRTNGVGLAGAAIAAVVLAGALGLT
                     WWRLFRGADLVHEAFSVVIPWRWALGDRPFVDEQNLVQTAGLLSYPFFKLYAVLGGND
                     VTGLVLYGRHVYLAVSVLAALAVFVLARRSLPSALAALVGAPFVTVLLFETPQLTANT
                     LCALSLTAGAALGAVTVLGGRRRCALAAGVAFGLACVAYPTVLLLAPFVAVFLAFSVG
                     DRSVIMLARGAPLRESEPTGRRAWSALSAWALGVAGVVIPVCALVFALAGRANLLRCW
                     DYTIDLARRLDQLGGTAKAAEIAGGFMALVAGQWYIVAAATVSYLVFRRRPGVGRWLL
                     LLTPPALWITATTSSLHVAGAVIVYALAAPYLYLFVPADRRPDGARLLVWVWAPALLV
                     GAMTAYTSGDGLVHAAVGLFPGIVTSGLFLAWGLAPLTPRRAGGRPWPALAALAAVVL
                     VTLAFQAQFQYGGVAGGERSVRMSAGPWKGIAVTPAQRARLHGFAADLARVGRSGDRL
                     LVYPWGAAYYLYWPGEVAANTYQLTVPGPGAPLPKATVSYFRRHREAPTLVGHLLDTG
                     GKTRAGLQAACGGLSYPPVVVAPGYALHRKPPLETTAEVLARLPRL"
     gene            2877..4148
                     /gene="dxr"
                     /locus_tag="VFZ86_15570"
     CDS             2877..4148
                     /gene="dxr"
                     /locus_tag="VFZ86_15570"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEX5643731.1"
                     /translation="MTERAPRHLLILGSTGSIGRQALEVLPAVPGLRLVGLAAGRDAA
                     AVAEQALAHGVRDVCLHDTGAAAEAAALAPELAVHAGEAGIRELITGAAERAAAAGAP
                     LTVLNGVVGAAGLRATMATLEAGATLALANKESMVAGGPFVLEAARRGGSLILPVDSE
                     HSALFQCIAAGGGEAGGDVVAAGPPRALEELLLTGSGGPFRGRAAADLSAVTAEQALA
                     HPNWSMGPKITIDSATLMNKGLELIEAHYLFGVPYERITVVLDPKSTVHSMARFSDGA
                     ILAHLGVPDMRTPIGYALAYPQRPPLPQVRRLDLFATAIAFERPDTGTFRCLALAEEA
                     GARAMVAEREAAAAGAGPRTVAAPVVLNAANEVAVAAFLAGRLSFLGIAEVVEASLER
                     LGAAPLASLDDVYAADADARRVAEDAIAARR"
     gene            4210..5454
                     /locus_tag="VFZ86_15575"
     CDS             4210..5454
                     /locus_tag="VFZ86_15575"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HEX5643732.1"
                     /translation="MTDDSRPTERSPEERDDRTRPPDADSTSPDDGAASGRVAAAPAP
                     RAVSPWSLLLGGALLVAIVFGQWVWLVAILGLALLITVHEFGHFIAAKAFGMRVEKFY
                     VGFPPAALRRTWGETEYGIGIIPLGGFCKISGMLPEEDVPEDTGDRVYYKKAIWKRNL
                     TIAAGPLMNFAAAAVILVLFVGIQGVATATLTLDEVVGDAPAAKAGLVAGDTLVGADG
                     QTWSTWDEASAYFRANPGKTISLTYEPKAGGLEKTVQLTLVENPEVPGSGYLGVRAGV
                     SRERPGPVEAVKLGLTGFGDVVVGTFTGFWWLLSGKISATGPDGAVGPVGIIDVSRQA
                     VEQNWYPILLAFLSFNLGLINLLPILPFDGGHIAMNVVEKIRGRRLDTRVFERLVAFG
                     TVLLVMLFIFLTFNDVKRLFGG"
     gene            5503..6501
                     /locus_tag="VFZ86_15580"
     CDS             5503..6501
                     /locus_tag="VFZ86_15580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015475380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX5643733.1"
                     /translation="MDGLTLVTGGAGFIGSHVVDALLASGSDVRVLDDFSTGKEENLD
                     DAWALAAQGGAGLQVMEGDVRDEVRMRDAMDGCTAVAHLAAIVSVPRSVEDPEGCGAV
                     THGGTVNALRQAVEAGAERFVLASSCAVYGDAAELPIAEVTAPRPLSPYAGAKLASER
                     ACVDAADAGQIVAACLRFFNVYGPRQDPGSAYSGVISRFISAAGGGAPVTIYGDGGQT
                     RDFVYVADVVEAVLAALGRPLSGASVVNIGTGRQTDLLQILGVVERLAGRRLERVYEP
                     AREGEVRDSRADAGRARWVLGWQARTGLDQGLAETWAWFAGAGGSAAPDARGGGRV"
     gene            6515..7594
                     /gene="ispG"
                     /locus_tag="VFZ86_15585"
                     /gene_synonym="gcpE"
     CDS             6515..7594
                     /gene="ispG"
                     /locus_tag="VFZ86_15585"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006722695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEX5643734.1"
                     /translation="MVGAVPVGGGAPVAVQSMTCTRTGDAEATLGQVRALAAAGCEIV
                     RVSLPTAEEAPAFTRVVREADVPVIADIHYDWRLALAAIAAGAAGVRINPGTMGEKHV
                     REIVHAAAEARVPAAPGRDAAPVAIRIGVNAGSLPRDLRGRAAGDVPGALVEAALRWA
                     TRFEEWGFTAYKLSVKSSSVPDTIAAYRLLAAQTDAPLHVGVTEAGTVWSGTIKSAVG
                     IGALLADGVGDTVRVSLTGDPVEEVRVAWQILAALGLRRRGPEVISCPTCGRTEVDIV
                     GLAEEVERRLGADPELAGRVLSVAVMGCRVNGPEEARHADYGIAAGAGEGVLFDHGER
                     VETLPEGQLVDALLTLVRAGSAGRS"
BASE COUNT          920 a         2781 c         2902 g         1155 t
ORIGIN      
        1 gcttcacacg caacgagctc acgaggttcc tcgaggcgaa tcgtgtggag acgcgcaacc
       61 tcttcagcgg caacctgctg cggcatcccg cgttcgagga catcgagcgc agggtcgtcg
      121 gcgaccttgc ggcgaccgac gccgtcatgg agcgcacgtt cttcgtcggc gtgtacccgg
      181 gcatcgacga gccgcgcctg cagtacatga tcgacgtctt cggtcgcttc atggacggcg
      241 aacgcgcggt gggagtcggc gccgacggcg tgccgactcc cggtcctgag gaccgctgag
      301 ccgcggggat ggccgtcgtc cgcaagatcg cctgcagggt cgccggcgtc accggccacg
      361 gcgagcgcgt ctacaccgtg gagctcgacc ccgtggcgcc ggcgccgcgc ttcaggcccg
      421 ggcagttcct gcacctcgcg ctcgacgact acgagccggg cggcttctgg ccggagtcgc
      481 gggtcttctc catcgcgagc gctccgcgcg cccgggaccg gctcgccatc acctacgcgg
      541 tgaagggccg cttcaccgcg cgcatggaag acgagctggc ggaggggcgg gaggtctggg
      601 tcaagcttcc ctacggggag ttcgtcatcg accagtcacg cgaggccgtc ctcttcgcgg
      661 gcgggacggg cgtgaccgcg ttcaccgcgt tcctgcagtc gctcgatgcc ggacgggcgc
      721 cgcgggtgct gctcttctac ggcgcccgga ccccggatct gttcgtgtac ggctccctgg
      781 tcgaggcctg tgcgcgtgag gtgccgtcac tctcgagcat actcgtcgcc gagacctccg
      841 gcggcgtcct gagcgtggcc cgggcgtggc cggcaatcaa ggagctggac gagcccatct
      901 tctacctcag cggcccgccc gcgatgctga cggcgctcac ggagcagctg ctcgagcgag
      961 gtgtctgcgc tgcgaccatc cgtaccgatg cgtgggagtg agcgcacgca ggatggttga
     1021 ggctccgccg gagggtcccg gcgcgctccc ggcgtcgggt gggcggaacg gccggacgaa
     1081 tggcgtcggc ctcgcgggtg ccgcgatcgc cgcggtcgtc ctcgcggggg cgttggggct
     1141 cacctggtgg cggctcttcc gcggcgccga cctggtccac gaggcgttct cggtggtgat
     1201 cccttggcgc tgggcgctcg gcgaccggcc gttcgtcgac gagcagaacc tggtgcagac
     1261 cgccggcctg ctctcctacc cgttcttcaa gctctacgcc gtgctcggcg ggaacgacgt
     1321 gaccggcctg gtcctgtacg ggcgccatgt ctatctggcg gtctccgtgc tggcggcgct
     1381 ggccgtgttc gtcctggccc ggcgttcgtt gccgtcagcg ctcgctgcgc tcgtcggcgc
     1441 gccgttcgtc accgtcctcc tgttcgagac gccgcagctc accgcgaaca cgctctgcgc
     1501 cctgtcgctg acggcggggg cggcgctcgg cgccgtcacc gtcctcggcg ggcgccgccg
     1561 ctgcgccctc gccgcagggg tggcgttcgg cctcgcctgc gtcgcgtatc ccacggtcct
     1621 gctcctggcg ccgttcgtcg cggtgttcct ggccttctcg gtcggcgatc gtagtgtgat
     1681 catgctcgcc cggggtgcgc cgctccgtga gagcgagccg acggggcggc gcgcctggag
     1741 cgcgctgagc gcctgggctc tgggcgtggc cggcgtggtc atccccgtgt gtgccctggt
     1801 gttcgccctg gccgggcgcg cgaacctcct gcgctgctgg gactacacga tcgacctggc
     1861 gcggcgcctg gaccagctcg ggggcacggc caaggcggcg gagatcgccg gaggcttcat
     1921 ggccctcgtg gccggtcagt ggtacatcgt cgccgcggcg acggtctcct acctcgtgtt
     1981 ccgccgccga ccgggcgtcg gacgctggct cctcctgctc acgccgccgg ctctctggat
     2041 cacggcgacc acgtcgtccc tgcacgtcgc cggcgcggtg atcgtctatg cgctggccgc
     2101 cccgtatctc tacctcttcg tgccggcgga cagaaggccg gacggcgccc gcctcctggt
     2161 ctgggtgtgg gctccggcgc tgctcgtcgg cgcgatgacc gcgtacacga gcggcgacgg
     2221 cctcgtgcac gccgccgtgg gcctgttccc ggggatcgtg accagcgggc tgttcctcgc
     2281 ctggggactg gcgccgctga cgccgcggcg cgccgggggt cgcccgtggc cggcgctggc
     2341 cgctctcgcg gcagtggtcc tcgtgacgct ggcgttccag gcgcagttcc agtacggcgg
     2401 cgtcgctggg ggggaacgct ccgtgcggat gagcgcgggg ccgtggaagg gcatcgccgt
     2461 gaccccggcg cagcgcgcgc gcctccacgg cttcgccgcg gatctggcgc gtgtaggtcg
     2521 gtcgggcgac cggctgctcg tctacccttg gggcgccgcg tactacctct attggccggg
     2581 cgaggtcgcc gccaacacct accagctgac cgttcccggc cccggagcgc cgctgcccaa
     2641 ggccaccgtc agctacttcc gtcgccaccg tgaggcgccg acgctcgtcg ggcacctcct
     2701 cgacaccggc gggaagaccc gtgccggact gcaggcggcg tgcggcgggt tgtcgtatcc
     2761 gcccgtggtg gtcgcgcccg ggtacgcgct ccaccgcaag ccgccgctcg agaccaccgc
     2821 cgaggtcctt gcgcgcctgc cgcgtctgtg agacgcgccg tggtaccctt gcgcccgtga
     2881 cggagcgtgc ccccagacat ctgctcatcc tcggctcgac cggctcgatc gggcgccagg
     2941 cgctcgaggt gctgcccgcc gtcccgggcc tgcgcctggt cgggcttgcg gcgggacggg
     3001 acgcggccgc tgtcgcggaa caggcgctcg cacatggggt gcgtgacgtt tgcctgcacg
     3061 acaccggggc cgccgcggag gccgcggcgc tggcgccaga gctcgcggtg cacgccggcg
     3121 aagccgggat ccgcgagctc atcaccggcg ccgccgagcg cgccgccgcc gcgggcgcgc
     3181 cgctgacggt gctgaacggc gtcgtcgggg cggccgggct gcgcgccacg atggcgacgc
     3241 tcgaggccgg cgccaccctg gcgctggcca acaaggagag catggtcgcg ggcgggccgt
     3301 tcgtgctcga ggcggcccgt cgaggcggct cgctgatcct gcccgtggac agcgagcaca
     3361 gcgcgctctt ccagtgcatc gccgccggcg ggggagaggc cggcggggac gtcgtggccg
     3421 ccggcccacc gcgcgcgttg gaggagctcc tgctgaccgg gtcgggcggc cccttccgcg
     3481 gccgcgcggc cgcggacctg tccgccgtca ccgccgagca ggcgctcgcg caccccaact
     3541 ggtcgatggg tcccaagatc accatcgact ccgcgacgct catgaacaag gggctcgagc
     3601 tcatcgaggc gcactacctc ttcggcgtgc cgtacgagcg catcaccgtc gtcctcgatc
     3661 cgaagagcac ggtgcacagc atggcgcgct tctccgacgg ggccatcctc gcgcacctgg
     3721 gagtgcccga catgcgcacg cccatcggct acgcgctcgc gtatccgcag cggccgccgc
     3781 tgccgcaggt acggcggctg gacctgttcg cgacggcgat cgcctttgag cggccggaca
     3841 cggggacctt ccgctgcctg gcgctcgccg aggaggcagg cgccagggcc atggtcgccg
     3901 agcgggaggc cgccgccgcg ggcgcgggtc cgcgcacggt cgccgcaccc gtcgtcctga
     3961 acgccgccaa cgaggtcgcg gtcgcggcgt ttctggccgg gcgtctgtcg ttcttgggga
     4021 tcgccgaggt cgtcgaggcg agcctcgagc ggctcggcgc cgcgccgctg gcgtcgctcg
     4081 acgacgtcta cgcggccgac gctgatgcgc gacgcgtggc ggaagacgcc attgccgcgc
     4141 ggcgctgaac ctcccgtccg tcggcggcgt taatgtggaa gtgacctcga ttgggtatcg
     4201 tgtgactaca tgaccgacga ctcgagaccg accgagcgct cccccgagga gcgcgacgac
     4261 cgtacgcgac cgcccgacgc cgactccacc tcgccagatg atggcgcggc gagcggccgc
     4321 gtcgctgcgg cgcccgcgcc gcgggccgtc agcccgtggt cgctgctgct cggaggcgcg
     4381 ctgctcgtgg cgatcgtctt cggccagtgg gtgtggctgg tggccatcct cgggctcgcc
     4441 ctgctgatca ccgtgcacga gttcggccac ttcatcgccg ccaaggcgtt cggcatgcgc
     4501 gtcgagaagt tctacgtcgg cttcccgccg gcggccctgc gccgcacctg gggcgagacc
     4561 gagtacggca tcggcatcat cccgctgggc ggcttctgca agatcagcgg catgctgccc
     4621 gaagaggacg tccccgagga caccggcgac cgggtctact acaagaaagc gatctggaag
     4681 cgtaatctca cgatcgccgc cggtccgctg atgaacttcg ccgcggccgc cgtcatcctc
     4741 gtcctcttcg tcggcatcca gggcgtggcc acggcgaccc tgacgctgga cgaagtggtc
     4801 ggagacgcgc cggcggccaa ggccggcctc gtggccggcg acaccctcgt cggggccgac
     4861 gggcagacgt ggtcgacgtg ggacgaggcg tccgcgtact tccgcgccaa ccccggcaag
     4921 accatctccc tgacctacga gcccaaggcg ggcgggctcg agaagaccgt gcagctgacg
     4981 ctggtcgaga acccggaggt gcccgggagc ggctacctcg gggtgcgcgc gggcgtctcg
     5041 cgcgagcggc cgggcccggt cgaggcggtc aagctcggcc tcaccggctt cggcgacgtg
     5101 gtcgtcggca cgttcaccgg cttctggtgg ctgctgagcg gcaagatcag cgcgacgggg
     5161 ccggacggcg ccgtaggccc ggtcggcatc atcgacgtca gccgccaggc ggtggagcag
     5221 aactggtatc cgatcctgct ggcgttcctc agcttcaacc tcggcctcat caacctgctg
     5281 ccgatcctgc cgttcgacgg cgggcacatc gccatgaacg tcgtggagaa gatccgcgga
     5341 cggcgtctcg acacgcgggt gttcgagcgg ctggtcgcct tcggcaccgt gctgctcgtc
     5401 atgctgttca tcttcctgac gttcaacgac gtcaagcggc tcttcggcgg ctgaggacgg
     5461 cgcgtcgcgg cgcggcgccc gcccgtgccg taggattggt gcatggacgg actcaccctg
     5521 gtcaccggcg gcgccgggtt catcgggtcg cacgtggtcg acgccctcct cgcgagcggc
     5581 tccgatgtgc gcgtgctcga cgacttcagc accggcaagg aggagaacct cgacgacgcc
     5641 tgggcgcttg cggcgcaggg cggcgccggc ctgcaggtca tggagggcga cgtgcgcgac
     5701 gaggtgcgca tgcgcgacgc catggacggc tgtacggccg ttgcgcatct ggcggcgatc
     5761 gtctcggtgc cgcgatcggt cgaggacccc gagggctgcg gcgcagtcac tcatggcggt
     5821 accgtgaacg cgctgcgcca ggccgtggag gccggcgccg agcggttcgt gctggcctcg
     5881 agctgcgccg tgtacggcga cgcggccgag ctgcccatcg ccgaggtgac cgcgccccgg
     5941 ccgctgagtc cctacgccgg cgccaaactg gcctccgagc gggcctgcgt ggacgccgcc
     6001 gacgccgggc agatcgtcgc cgcctgcctg cgcttcttca acgtgtacgg cccgcggcag
     6061 gatcccggct cggcctactc cggggtcatc agccgcttca tctccgcggc cgggggcggc
     6121 gcaccggtga ccatctacgg tgacggcggg cagacgcgcg acttcgtgta cgtcgccgac
     6181 gtcgttgagg ccgtgctcgc ggcgctgggg cggccgctca gcggcgcgtc ggtggtcaac
     6241 atcggcaccg gccggcagac cgacctgctg cagatcctcg gcgtcgtcga gagactcgcc
     6301 gggcggcggc tggagcgggt gtacgagccc gcccgtgagg gcgaggtgcg cgactcgcgg
     6361 gccgacgcgg gccgcgctcg ctgggtgctc ggctggcagg cgcggaccgg cctcgaccag
     6421 ggtctcgccg agacctgggc gtggttcgcc ggcgccgggg gaagtgcggc gccggacgcg
     6481 cgcggaggcg gccgtgtcta gcgcgcgccg cgtcatggtc ggcgccgtcc ccgtcggcgg
     6541 cggcgcccca gtggcggtgc agtcgatgac gtgcacgcgg accggcgacg cggaggcgac
     6601 gctgggacag gtgcgcgctc tggccgccgc cggctgcgag atcgtgcgcg tgagcctgcc
     6661 gacggctgag gaggctccgg cgttcacgcg cgtcgtgcgc gaggcggacg tgccggtcat
     6721 cgccgacatc cactacgact ggcggctggc cctggcggcg atcgccgccg gggcggcggg
     6781 cgtgcgcatc aaccccggga cgatggggga gaagcacgtc cgtgagatcg tgcacgcggc
     6841 ggcggaggcc cgggtgcctg cggcgccggg ccgggacgcc gcgcccgtcg ccatccgcat
     6901 aggggtgaac gccgggtcct tgccgcgcga cctgcggggg cgcgccgccg gcgacgtgcc
     6961 cggcgcgctc gtcgaggcgg cgctgcgttg ggccacgcgg ttcgaggagt ggggcttcac
     7021 cgcctacaag ctcagcgtga agtcgtcatc cgtccccgac accatcgccg cgtaccggtt
     7081 gctggcggcg cagacggacg cgccgctcca cgtcggcgtg acggaggcgg gcaccgtgtg
     7141 gagcgggacg atcaagagcg ccgtcggcat cggcgcattg ctcgccgacg gcgtcggcga
     7201 cacggtccgg gtgagcctga ccggcgatcc ggtcgaggag gtcagggtcg cctggcagat
     7261 cctcgccgcg ctggggctgc ggcggcgcgg ccccgaggtc atctcctgcc cgacctgcgg
     7321 ccgcaccgag gtcgacatcg tcgggctcgc cgaagaggtc gagcgccggc tcggcgccga
     7381 cccggagctc gccggccgcg tcctcagcgt cgccgtgatg ggctgccgcg tgaacggccc
     7441 cgaggaggcg cggcacgccg actacggcat cgccgccggc gccggcgagg gcgtgctctt
     7501 cgaccacggc gagcgcgtcg agacgctgcc cgaggggcag ctggtcgacg cgctcctcac
     7561 gctggtccgc gccggctcgg ccggacgcag ctgagcgcga cgcggccttt ctcccgaccg
     7621 cgccgccaag gcggtccccc cactccagac ggatgtacca gcgagcacgg cgccattctt
     7681 gccgacatcc ttctgcagat gcgttccagg acgcggggac aggaggctgg gcatgacggc
     7741 gacgggggac gggaagcg
//
LOCUS       DASUMX010000329         3267 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_561852, whole genome shotgun sequence.
ACCESSION   DASUMX010000329 DASUMX010000000
VERSION     DASUMX010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3267
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561852"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="VFZ86_15590"
     CDS             <1..313
                     /locus_tag="VFZ86_15590"
                     /inference="COORDINATES: protein motif:HMM:NF014679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEX5643735.1"
                     /translation="TLDRLHLAPIIVFDEHGKPGKGGAALGFDRALNAIVKRAVSFAD
                     GAPARAMVVHSGDRTGAGFVAARLEERLGGEVPVIRAGAVLTTHVGLGSVTVAVRRLG
                     G"
     gene            444..1601
                     /locus_tag="VFZ86_15595"
     CDS             444..1601
                     /locus_tag="VFZ86_15595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012981665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HEX5643736.1"
                     /translation="MAVRIGVPRETATGETRVALVPAVAEKFAALGAEVLVERGAGTL
                     SHIRDEDFTAATLVDGPAEVLAADIVLKVDPPTPAEVEQMREGAVVLGLLQPYKADDA
                     IKALRDHGMTSFALELLPRITRAQSMDALTSQASIAGYKAVLMSACMAGRYYPMLMTP
                     AGTLRPARIVVLGVGVAGLQAIATARRLGAVVEAYDVRAVTREQVQSLGGRFIDTGID
                     AEVEGGYARELTDEEKAKAKEIVDSHIIEADAVITTAAIPGRPSPKLITADVVEKMKS
                     GAVIIDMAAEGGGNCELTRPGEQYNHPNGVFIYGPLNIPGMLPVHASDQYARNLMHFL
                     TPFIKDGEFTLDWDDEVLAGSVLTRDGAIVNQGVRERIEGPDAPDDEGGAS"
     gene            1598..1936
                     /locus_tag="VFZ86_15600"
     CDS             1598..1936
                     /locus_tag="VFZ86_15600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018860840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HEX5643737.1"
                     /translation="MITGFAAVYIFILAAFVGYEVISRVPVILHTPLMSGSNFVHGIV
                     VVGAMVALGAAETTPERIVGFLAVLLGAANAVGGYAVTERMLDMFKSSGDQKKGAASG
                     AGAAKGGGDA"
     gene            1929..>3267
                     /locus_tag="VFZ86_15605"
     CDS             1929..>3267
                     /locus_tag="VFZ86_15605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020395521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEX5643738.1"
                     /translation="MRENLIQIAYIAAAVLFILGLKSMSSPRTARRGIIWAGVGMLVA
                     TVITFATPELGNFVWMTLALVIGGGVAYVAARRVAMTDMPQMVAIYNGMGGGAAALIA
                     AVEFMHPGELPMSFAILGIAGALIGSVSFSGSMIAFAKLQGIIKKSFLFPGQNILNLL
                     VLAAAVVLAVIAVLQHTGNMGGNESLVLVGFFALSLLYGVLMTLPIGGADMPVVISLY
                     NALTGLAVGFEGYVLGNGALIIAGIVVGAAGTLLTQLMAKAMNRSIGNVLFAGFGAKG
                     GQELEAIEGSMKESSPDDAAVTLAFAQKVIIVPGYGMAVAQAQHKVWELAKLLGDRGI
                     QVKFAIHPVAGRMPGHMNVLLAEAGVPYDMIYDIDEINGEFPTADAAIVIGANDVVNP
                     AARDDPGSPIHGMPILDVDKAVQAFVIKRGRGAGFAGIENKLFYADNTRMLYGD"
BASE COUNT          499 a         1157 c         1134 g          477 t
ORIGIN      
        1 gacgctcgac cggcttcacc tcgcgccgat catcgtcttc gacgagcacg gcaagccggg
       61 caaagggggc gcggcgctcg ggttcgaccg cgccctgaac gcgatcgtga agcgcgccgt
      121 gagcttcgcg gacggcgcgc cggcccgcgc catggtcgtc cacagcggcg accgcacggg
      181 cgcgggcttc gtggccgcgc gcctcgagga gcgcctgggc ggcgaggtgc ccgtcatccg
      241 cgccggcgcg gtgctcacca cgcacgtcgg cctcgggtcc gtgacggtcg ccgtgcgccg
      301 cctcggcggc tgacacgcgc gcggccggcg gcctgcccgg cctcgcctcc ccactttgca
      361 ctgcgcgcag aggtttttcg ccgccgaccc ttgggaagtg acttgacggg cccggattgg
      421 gcccgcccca cgaggagggt tcgatggcag tccgcattgg cgtgccccgg gagacggcga
      481 cgggggagac gcgcgtggcc ctggtcccgg cggtggcgga gaagttcgcc gcgctgggcg
      541 ccgaggtgct ggtcgagcgc ggcgccggca cgctcagtca catccgcgac gaggacttca
      601 cggcggcgac cctggtcgac ggtccggcag aggtcctcgc cgccgacatc gtcctgaagg
      661 tggacccgcc cacgccggct gaggtcgagc agatgcgcga gggcgccgtc gtgctcggcc
      721 tgctgcaacc ctacaaagcg gacgacgcca tcaaggcgct ccgtgaccac gggatgacga
      781 gcttcgcgct cgagctcctg ccgcgcatca cgcgggcgca gtccatggac gcgctgacga
      841 gccaggcctc gatcgccggc tacaaggccg tgctgatgag cgcctgcatg gcgggccggt
      901 actacccgat gctgatgacg cccgcgggca cgctgcggcc cgccaggatc gtcgttctcg
      961 gagtgggcgt ggccgggctg caggcgatcg ccaccgcgcg ccgcctcggc gccgtggtcg
     1021 aggcgtacga cgtgcgcgcc gtgacgcgcg aacaggtgca gtcactgggc ggccgcttca
     1081 tcgacaccgg catagacgcc gaggtggagg gcggctacgc tcgcgagctc accgacgagg
     1141 agaaagccaa ggcgaaggag atcgtcgaca gccacatcat cgaggccgac gccgtgatca
     1201 cgacggccgc catcccgggc aggccgtctc cgaagctcat cacggccgac gtggtggaga
     1261 agatgaagtc cggcgcggtg atcatcgaca tggccgccga gggcggcggc aactgcgagc
     1321 tcacccggcc gggcgaacag tacaaccacc cgaacggcgt gttcatctac gggccgctca
     1381 acatccccgg catgctgccg gtgcacgcga gcgaccagta cgcgcgcaac ctgatgcact
     1441 tcctcacgcc gttcatcaag gacggtgagt tcacgctcga ctgggacgac gaggtcctgg
     1501 ccggcagcgt gctgacccgc gacggtgcga tcgtgaacca aggcgtccgc gagcggatag
     1561 agggaccgga cgcgcccgac gacgagggag gcgcgtcatg atcaccggct tcgccgcggt
     1621 ctacatcttc atcctcgccg ccttcgtcgg ctacgaggtc atctcacgcg tgccggtgat
     1681 cctgcacacg ccgctgatga gcggctccaa cttcgtgcac ggcatcgtcg tcgtcggcgc
     1741 catggtggcg ctcggggcgg cggagacgac ccccgagcgc atcgtcggct tcctcgccgt
     1801 gctgctcggc gcggccaacg cggtcggcgg ctacgccgtg accgaacgga tgctcgacat
     1861 gttcaagagc tccggcgacc agaagaaggg tgcggcgtcc ggcgccggcg cggcgaaggg
     1921 tggcggcgat gcgtgagaac ctgatccaga tcgcctacat cgccgccgcc gtcctcttca
     1981 tcctcggcct caagagcatg agctcgccgc gcaccgcgcg gcgcggcatc atctgggccg
     2041 gcgtcggcat gctcgtcgcc accgtcatca ccttcgcgac gccggagctc ggcaacttcg
     2101 tctggatgac gctcgccctg gtcatcggcg gcggcgtcgc ctacgtggcg gcgcggcgcg
     2161 tcgccatgac cgacatgccg cagatggtcg cgatctacaa cggcatgggc ggcggcgccg
     2221 cggctctgat cgccgccgtc gagttcatgc acccgggcga gctgccgatg agcttcgcca
     2281 tcctcggcat cgccggcgcc ctcatcggct ccgtgagctt cagcggcagc atgatcgcgt
     2341 tcgccaagct gcaggggatc atcaagaagt cgttcctgtt cccgggccag aacatcctga
     2401 acctgctggt gctggccgcc gccgtggtcc tcgccgtcat cgccgtgctg cagcacacgg
     2461 gcaacatggg cggcaacgag agcctcgtcc tcgtcggctt cttcgccctc tcgctgctct
     2521 atggcgtgct catgacgctg cccatcggcg gcgccgacat gcccgtcgtc atcagcctgt
     2581 acaacgctct caccggcctg gcggtcggct tcgagggcta cgtgctcggc aacggcgccc
     2641 tgatcatcgc cggcatcgtc gtcggcgcgg ccggcaccct gctcacgcag ctgatggcca
     2701 aggccatgaa ccgctccatc ggcaacgtcc tgttcgcggg gttcggcgcc aagggagggc
     2761 aggagctgga ggcgatcgag ggttcgatga aggagtcgag cccggacgac gccgcggtca
     2821 cgctcgcgtt cgcgcagaag gtcatcatcg tgcccggcta cggcatggcc gtcgcccagg
     2881 cgcagcacaa ggtctgggag ctggccaagc tgctcggcga ccgcggcatc caggtgaagt
     2941 tcgccatcca cccggtcgcc ggccgcatgc cggggcacat gaacgtcctg ctcgccgagg
     3001 ccggcgtgcc ctacgacatg atctacgaca tcgacgagat caacggcgag ttcccgaccg
     3061 ccgacgccgc catcgtgatc ggcgccaacg acgtcgtgaa cccggcggcg cgcgacgacc
     3121 ccggcagccc gatccacggc atgccgatcc tcgacgtcga caaggcggtc caggcgttcg
     3181 tgatcaagcg cgggcgcggc gccggcttcg ccggcatcga gaacaagctg ttctacgcgg
     3241 acaacacgcg catgctgtac ggcgacg
//
LOCUS       DASUMX010000330         6663 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_851204, whole genome shotgun sequence.
ACCESSION   DASUMX010000330 DASUMX010000000
VERSION     DASUMX010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 6663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6663
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_851204"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..948
                     /gene="gpmI"
                     /locus_tag="VFZ86_15610"
     CDS             <1..948
                     /gene="gpmI"
                     /locus_tag="VFZ86_15610"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HEX5643739.1"
                     /translation="DRVKLAYDALVHAQGFFAAGAQAAVDAAYARGENDEFVRPTIVA
                     PEHDARVRDGDVCIFFNFRPDRARELTRALTEPGFAEFDRGARPPSTDFVTMTQYKKE
                     FPLPVAFPQEHPEHVLADVLAEHGLKQLHIAETEKYAHVTFFFNGGVEKAVEGEERVL
                     VPSPREVPTYDHKPEMSAIGVTDELVARLETDVFDFVVVNYANADMVGHTGVIPAAVR
                     AVETVDACLGRVVEATRRLGGVCLVTADHGNSDHMLEPDGSPNTAHSTNLVPFVATVP
                     GARVREGGRLCDLAPTILARLGVEQPPEMTGRDLLEPAG"
     gene            1078..1299
                     /gene="secG"
                     /locus_tag="VFZ86_15615"
     CDS             1078..1299
                     /gene="secG"
                     /locus_tag="VFZ86_15615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEX5643740.1"
                     /translation="MLNAILVAIQLGLAILVIVLILLHSGKDAGMASVTGFSFASSAS
                     VMERNLTRYTIIVGVLFFLNTFVLIWRLG"
     gene            1418..3088
                     /locus_tag="VFZ86_15620"
     CDS             1418..3088
                     /locus_tag="VFZ86_15620"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5643741.1"
                     /translation="MPPRPSRIAAFLLAAVLLLVAAASLAGCTDDSSDAGPAQNDEGT
                     PRPGGTFVFPLPGDPVSIEPLNAQDASGLQVAHQVFEGLTRWVRDDDGVLVTEPGIAA
                     TWESADAQTWVFHLKQGVTFQAPVSRPVTAQDFVDSWARVTDPANQSYVAYILAPIEG
                     CDDRGYQTDPAQGLTGVTALDDQTLQVVLRYPFADFPATLGHPVAAVTPVEYIDQVGA
                     KAYAKKPVGTGPFSVRSWRHGKELRLDRNATYWDAEQPAWLDAVELPIYEDVADMWAD
                     FEAGELDCSQAPSDQVATVEASTEVADGTWTAGTWPAAALYFVGVNMTDPTLGTSLDL
                     RKAISQSADAQSVVEEVSAGVAEVAAGYVPPGIPGHMGAQNPYPYDVESAASLVTTMG
                     GAPTLAYWYDVNEDHQAIADVLVTGWAQAGLSVKASAYEWGTFLEKLSRGDRGSGSQL
                     FRVAWIADYPAMDAFLYPLFQSDQSPAGSYTFYSNQGVDDLLQKARATVDAQQRHNLY
                     AQAEKLILTDMPAAPLYFYRYFRVVGPRLHGFSVDPMGTTDMRAVWLE"
     gene            3280..4977
                     /locus_tag="VFZ86_15625"
     CDS             3280..4977
                     /locus_tag="VFZ86_15625"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX5643742.1"
                     /translation="MKKPVRRWRGLLILAAVLIVAAMLLAACGSDEPSTDASPTAGGE
                     PKAGGEYNFPLGAEPISIEPLNTQESEGWHVEHQIFQGLYILQLQDDGTTKAVPDLAE
                     TTEVNDDATVFTFKIREGVMFQPPVSREVTAQDFVDAWSYNAQAENESATTYIIAPIE
                     GIDPETGYARGPTLDGVKALDKYTLEVTLQYPFADFAATLVHPITQVFPVDYVEEIGR
                     KAFFDKPVGTGPYMVEEWKHNQYITLAQNPEYWNPDGASPNSAGPGYFETINMPIYAD
                     QNTEWLAFQKGDIDYSTIPTGNFQASQNDPKVADGTWTVKAYPSTSVYFISVAMNKPT
                     LGGDENLPIRAALNMAADREAVSNIVNEGISIPSDSIVPVTIPGYTPGNNPYPYDPAN
                     AQAKLDEFTGTLPSEIPYWFNSGSGHDKIAEALVAGWQKAMPSLKFKLSGVETNSYWT
                     QAGENKFDGLVRMGWIADYPSIDNFIYLFTTEGGEYGSYSWYSNPEVDKLFQEARSTT
                     DEAQRLALYNEAEKLILADAPVVPVYTYRDARATNNRIGGFTYNAFAQINMWEMWVK"
     gene            5133..6089
                     /locus_tag="VFZ86_15630"
     CDS             5133..6089
                     /locus_tag="VFZ86_15630"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5643743.1"
                     /translation="MIAYIIRRLLQLVVVLFGVTLITFGMLKLVPGDPAVALAGKGAS
                     PEKIAFVRHERGLDRPFYVQYYKYVERLLHGDLGESLYTSRPVAEMIKDAAPVTIQLA
                     VAAVLIELLGIPLGVYSALRQYSFWDTALTTGALILWGIPVFVLGYFAMYIFGLKLDW
                     LPIGGVGPTVLGIIPSTWESWQTLILPALTLGLIEVAYISYMQRASMLEVSRSDFIRT
                     ARAKGLSERKVIWGHGFKNAVIPVMTIAGIDLGALIGGAIITETVFNRPGIGMMIYQA
                     INQRDTAIIAGGVLFATFFFVFANLLVDLGYAWVDPRVRLED"
     gene            6092..>6663
                     /locus_tag="VFZ86_15635"
     CDS             6092..>6663
                     /locus_tag="VFZ86_15635"
                     /inference="COORDINATES: protein motif:HMM:NF024313.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643744.1"
                     /translation="MPDWDERKQEGRETYGSAWVTEADSVDARRTEEVVEEVIEEQAK
                     PLGLWADTWRRLRRNHLALVGLGIIIVFVSIGTVEAVFYQMHWHVRASDEATASDAPG
                     YLAPYDPNMVNYDLSPSGLGSPPSWSHPFGTDYLGRDVLSRTLVATRVAMLVGIIAVA
                     IALTLGLILGPVSGYYGGVVDSIIMRAADI"
BASE COUNT         1098 a         2431 c         2132 g         1002 t
ORIGIN      
        1 gaccgcgtca agctcgccta cgacgccctc gtgcacgctc agggcttctt cgccgccggc
       61 gcgcaggccg cggtggacgc ggcgtacgcc cgcggcgaga acgacgagtt cgtgcgcccg
      121 acgatcgtcg cgccggagca cgacgcgcgc gtgcgcgacg gcgacgtgtg catcttcttc
      181 aacttccgtc cggacagggc gcgtgagctc acgcgcgccc tcaccgaacc cggcttcgcc
      241 gagttcgacc ggggcgcccg cccgcccagc accgacttcg tgacgatgac gcagtacaag
      301 aaggagttcc ctctgccggt ggcgttcccg caggagcacc cggagcacgt gctggcggac
      361 gtgctcgccg agcacggcct gaagcagctg cacatcgccg agacggagaa gtacgcgcac
      421 gtgacgttct tcttcaacgg cggggtcgag aaggcggtcg agggggagga gcgcgtcctc
      481 gtgcccagtc cgcgcgaggt gccgacctac gaccacaagc ccgagatgag cgctatcggc
      541 gtcaccgacg agctcgtcgc cagattggag accgacgtct tcgacttcgt ggtcgtcaac
      601 tacgcgaacg ccgacatggt cgggcacacc ggggtcatcc ccgccgccgt gcgcgccgtg
      661 gagaccgtgg acgcgtgtct cggccgcgtc gtggaggcca cgcggcgcct cgggggcgtg
      721 tgcctggtga ccgccgacca cggcaactcg gaccacatgc tcgagccgga cggcagcccc
      781 aacacggcgc acagcaccaa cctcgtgccc ttcgtcgcca ccgtccccgg cgcgcgcgtg
      841 cgcgaggggg ggcgcctctg cgacctcgcg ccgacgatcc tcgcccggct gggggtggag
      901 cagccgccgg agatgaccgg ccgcgacctg ctcgagccgg ccggctgacc gctcgcacgc
      961 ggcgcccgcc gccggccctt caccggccgt catcgcggcg cggtttggag ggcgcctgcc
     1021 gaggtgcaac aatgatatgc tcgcacacgt ccaagcccgc cttcaggggg agaagacttg
     1081 ctcaacgcca tcctcgtcgc catccagctc ggcctcgcga tcctcgtgat cgtgctcatc
     1141 ctgctgcact ccggcaagga cgccggcatg gccagcgtca ccgggttctc gttcgcctcg
     1201 tccgcgagcg tcatggagcg caacctcacg cgctacacga tcatcgtggg cgtgctgttc
     1261 ttcctgaaca cgttcgtcct catctggagg ctgggctgac gccgtccgcg tgcagcgccg
     1321 cccgggcgct cccgcgagcg tcgtccgccc gccccatgag cggccgggcg cgccacttcg
     1381 gccgggccgg cggaggtgtg tccgcgggca tccactgatg ccgccgcgcc cgtccagaat
     1441 cgccgcgttc ctgctggccg ccgtcctgct gctcgtggcg gcggcctcgc tcgccggctg
     1501 caccgacgac tcctcggacg ccgggccggc ccagaacgac gaggggacgc ccagaccggg
     1561 cggcaccttc gtgttcccgc tgccgggcga tccggtgtcc atcgagccgc tgaacgccca
     1621 ggacgcctcc ggcctgcagg tggcgcacca ggtgttcgag ggcctcacca ggtgggtgcg
     1681 cgacgacgac ggcgtgctcg tcaccgaacc cggcatcgcc gccacctggg agtccgcgga
     1741 cgcgcagacg tgggtgttcc acctcaagca aggggtgacg ttccaggcgc cggtgagccg
     1801 gccggtgacg gcgcaggact tcgtcgactc gtgggcgcgc gtcaccgacc ccgcgaacca
     1861 gtcgtacgtg gcctacatcc tcgcgcccat cgagggctgc gacgaccgcg gctaccagac
     1921 cgatcctgcg caggggctca ccggggtcac ggccctcgac gaccagacgc tgcaggtcgt
     1981 cctgcgctac cccttcgccg acttcccggc gacgctcggg catccggtcg ccgcggtgac
     2041 cccggtggag tacatcgacc aggtcggcgc caaggcttac gccaagaagc ccgtgggcac
     2101 cggtccgttc agcgtgcgct cgtggcggca cggcaaagag ctgcgcctcg acaggaacgc
     2161 gacctactgg gacgccgagc agccggcgtg gctcgacgcc gtcgaactgc ccatctacga
     2221 ggacgtcgcg gacatgtggg cggacttcga ggccggcgag ctcgactgct cccaggcccc
     2281 gagcgaccag gtcgccaccg tcgaggcgag cacggaggtc gccgacggca cctggacggc
     2341 cggcacctgg ccggcggccg ccctctactt cgtcggtgtc aacatgacgg accccacgct
     2401 cggaacaagc ctcgacctgc gcaaggcgat cagccagtcc gcggacgccc agtcggtggt
     2461 cgaggaggtc agcgcgggcg tcgccgaggt ggccgccggc tacgtgccgc ccggcatccc
     2521 ggggcacatg ggcgcgcaga acccgtatcc ctacgacgtg gagagcgccg cctccctggt
     2581 gacgacgatg ggcggcgccc ccactctcgc gtactggtac gacgtcaacg aggatcacca
     2641 ggcgatcgcc gacgtgctgg tgacgggatg ggcgcaggcg ggtctcagcg tgaaggccag
     2701 cgcgtacgag tgggggacct tcctggagaa gctctcacgc ggcgacagag gcagcgggag
     2761 ccagctcttc cgtgtcgcct ggatcgccga ctacccggcg atggacgcgt tcctgtaccc
     2821 gctgttccag tcggaccagt cacctgccgg cagctacacg ttctactcca accagggcgt
     2881 ggacgacctc ctgcagaagg cgcgcgccac cgtggacgcc cagcagcgcc acaacctgta
     2941 cgcacaggcc gagaagctca tcctcaccga catgccggcg gcgccgctgt acttctaccg
     3001 ctacttccgg gtcgtcggcc cacgcctgca cggcttcagc gtcgacccca tgggtaccac
     3061 cgacatgcgg gccgtctggc tcgagtagca cctgcttcgg caggggtttg catatgggag
     3121 taaagttgac aagtcggcgc tgagagcgcc acagtgccca cgaaggtagg gggaggtgcg
     3181 tcaaagaggt cgtaatggcc gcggttccgt gagggcggcg cggccgtaag aggctccggg
     3241 gggagtcttc tgtctcgttc agtcaccaag gaggtcaccg tgaagaaacc cgtgaggaga
     3301 tggcgcggcc tgttgatcct ggccgccgtc ctcatcgtcg ccgcgatgct gctggccgca
     3361 tgcggcagcg acgaaccgtc caccgacgcc agtccgacgg ccggcggcga gcccaaggcc
     3421 ggcggcgagt acaacttccc gctcggcgcc gagccgatca gcatcgagcc cctcaacacg
     3481 caggagtcgg agggatggca cgtcgagcac cagatcttcc aggggctcta catcctgcag
     3541 ctccaggacg acggcacgac caaggcagtg ccggacctcg ccgagacgac cgaggtcaac
     3601 gatgacgcga cggtcttcac gttcaagatc cgtgagggcg tcatgttcca gccgcccgtc
     3661 agccgtgaag tgacggcgca ggacttcgtc gacgcctgga gctacaacgc ccaggccgag
     3721 aacgagtcgg ccacgaccta catcatcgcc ccgatcgagg gcatcgaccc cgagaccggc
     3781 tacgcgcgcg gcccgacgct ggacggcgtc aaggccctcg acaagtacac cctcgaggtc
     3841 acgctccagt acccgttcgc cgacttcgcg gcgaccctgg tgcaccccat cacgcaggtg
     3901 ttccccgtcg actacgtcga ggagatcggc cgcaaggcgt tcttcgacaa gcccgtcggc
     3961 acgggcccgt acatggtcga ggagtggaag cacaaccagt acatcacgct ggcccagaac
     4021 cccgagtact ggaacccgga cggcgcctca cccaactcgg ccggtcccgg ctacttcgag
     4081 accatcaaca tgccgatcta cgccgaccag aacaccgagt ggctcgcctt ccagaagggc
     4141 gacatcgact actccaccat cccgacgggc aacttccagg cctcccagaa cgaccccaag
     4201 gtcgccgacg gcacctggac cgtcaaggcc tacccgagca cgtccgtcta cttcatcagc
     4261 gtcgcgatga acaagcccac gctcggcggc gacgagaacc tgccgatccg cgccgccctc
     4321 aacatggcgg ccgaccgcga agccgtcagc aacatcgtca acgagggcat ctcgatcccc
     4381 tcggactcca tcgtgccggt cacgatcccg ggctacacgc cgggcaacaa cccgtatccc
     4441 tacgacccgg cgaacgcgca ggccaagctt gacgagttca cgggcacgct cccgagcgag
     4501 atcccctact ggttcaactc gggctccggc cacgacaaga tcgccgaggc cctggtcgcc
     4561 ggctggcaga aggccatgcc gtcgctcaag ttcaagctca gcggcgttga gaccaacagc
     4621 tactggactc aggcaggcga gaacaagttc gacggcctgg tgcgcatggg ctggatcgcg
     4681 gactacccgt cgatcgacaa cttcatctac ctgttcacca ccgagggcgg cgagtacggc
     4741 agttacagct ggtacagcaa ccccgaggtg gacaagctct tccaggaggc gcgcagcacc
     4801 accgacgagg cgcagcgcct cgccctctac aacgaggccg agaagctcat cctcgccgac
     4861 gctcccgtcg tcccggtcta cacgtaccgt gacgcccgtg ccaccaacaa ccgcatcggc
     4921 ggcttcacct acaacgcatt cgcgcagatc aacatgtggg agatgtgggt gaagtaggcc
     4981 tggctctggt tgctccccgc gagcggcctg agtagactcc acgcagtgat acatgccaga
     5041 cggcccgcgg cgaacgccgc gggccgtctg gcatcaccga acagccgtgt ccgtcccgga
     5101 cgcgaggggt ctgcccgagt gagaacgctc tcttgatcgc gtatatcatc cgacgcctgc
     5161 tgcagcttgt cgtcgtgctg ttcggggtca cgctcatcac cttcggcatg ctgaagctgg
     5221 tacccggcga ccccgccgtc gctctcgccg gcaagggcgc ctcacccgag aagatcgcct
     5281 tcgtgcgtca cgagcgcggc ctcgaccggc cgttctacgt gcagtactac aagtacgtcg
     5341 agcgactcct gcacggcgac ctcggcgagt ccctgtacac gtcccggccg gtcgccgaga
     5401 tgatcaagga cgccgcgccg gtcaccatcc agctcgccgt cgcggcggtg ctcatcgagc
     5461 tcctcgggat cccgctcggc gtctactccg cgttacgcca gtactcgttc tgggacacgg
     5521 cgctcaccac cggggcgctc atcctctggg gcatcccggt gttcgtgctc ggctacttcg
     5581 ccatgtacat cttcgggctc aagctcgact ggctccccat cggcggcgtc gggcccactg
     5641 tgctcggcat catcccgtcg acctgggaga gctggcagac gctcatcctc ccggcgctca
     5701 ccctcggcct catcgaggtc gcctacatct cctacatgca gcgcgcctcg atgctcgagg
     5761 tgagccgctc ggacttcatc cgcaccgctc gcgccaaggg cctcagcgag cgcaaggtca
     5821 tctggggcca cggcttcaag aacgccgtga tccccgtcat gaccatcgcc ggcatcgacc
     5881 tcggcgccct catcggcggc gccatcatca ccgagacggt gttcaaccgc cccggcatcg
     5941 gcatgatgat ctaccaggcc atcaaccagc gcgacacggc catcatcgcc ggcggcgtgc
     6001 tcttcgcgac gttcttcttc gtgttcgcca acctgctcgt cgacctcggc tacgcgtggg
     6061 tggacccgcg cgtccgcctc gaggattagc cgtgcccgac tgggacgagc gcaagcagga
     6121 gggcagggag acctacggct cggcctgggt caccgaggcc gactccgtgg acgcgcgacg
     6181 caccgaggag gtcgtcgagg aggtcatcga ggagcaggcc aagccgctcg gcctgtgggc
     6241 cgacacgtgg cggcggctcc ggcgcaacca cctcgccctc gtcggcctcg gcatcatcat
     6301 cgtcttcgtc agcatcggga cggtcgaggc cgtcttctat cagatgcact ggcacgtccg
     6361 cgccagcgat gaggccacgg cctccgacgc gccggggtac ctcgccccgt acgacccgaa
     6421 catggtcaac tacgacctct cgccctccgg gctcggctca ccgccgtcct ggtcacaccc
     6481 gttcggcacc gactacctcg gccgcgacgt gctctcgcgc acgctggtcg ctacccgcgt
     6541 cgccatgctc gtcggtatca tcgccgtcgc catcgcgctc acgctggggc tgatcctcgg
     6601 gccggtctcg gggtactacg gcggcgtcgt cgactcgatc atcatgcgcg ccgccgacat
     6661 ctt
//
LOCUS       DASUMX010000331         6229 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_562279, whole genome shotgun sequence.
ACCESSION   DASUMX010000331 DASUMX010000000
VERSION     DASUMX010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 6229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6229
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562279"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="VFZ86_15640"
     CDS             complement(<1..430)
                     /locus_tag="VFZ86_15640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643745.1"
                     /translation="MHDVLSSRVARDRVRARARLTARMAVSRARGRPHTLSHLVTARC
                     NGSCATCLWRAGAGGSAAVGATAAPAGERAAGDGRAAAGELGTAAVRRLYAEAGRAGM
                     AQLVVWGGEPLLRRDLPELLLAARRSGLFVTLISNGWFAAE"
     gene            541..1104
                     /locus_tag="VFZ86_15645"
     CDS             541..1104
                     /locus_tag="VFZ86_15645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEX5643746.1"
                     /translation="MSAELTGKRIAILATDGVEGVELTSPREALEQAGATVELLAPED
                     GSIQLWDHSEKGGTLPVDRAVSEADPQTYDGLVLPGGVRNADHLRMDAASVRFVEALA
                     RRGTPIGVICHGAWILIEAQAVDGRRLTSYPSLRTDLQNAGAQWVDQEVVNDDGLVSS
                     RRPDDLPAFNAKIVEEFAEGAHGRRAA"
     gene            1209..1790
                     /locus_tag="VFZ86_15650"
     CDS             1209..1790
                     /locus_tag="VFZ86_15650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643747.1"
                     /translation="MTEQEVFILADQALNAVVDQIRDDQWDLVVPDDMTRTPGITLRQ
                     TIDYHAYDDAWVPHVLAGETMEDVGDRYDGDLLGDHPKLNFASIAETAVLAVRDFNDP
                     DRIVHLSYGDWPAREYLKHITSFRGLRAYDIAKFIGADTTLPPDLVRGLWDEIAPSAE
                     EWRKLGVYGPAVPVPDDAPLQQKLLGLTGRQPG"
     gene            1935..3119
                     /locus_tag="VFZ86_15655"
     CDS             1935..3119
                     /locus_tag="VFZ86_15655"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5643748.1"
                     /translation="MDKTSRRLALALIAVLVLTFAAVGLAACGGDEAADDGAGETAAP
                     FKIGVAGPMTGQYATYGASHKAGAEIAMEELNADGGVNGGEVSIALGDDLGDPKEAVL
                     VAQKFIDDTELVFVNGHQFSGATIAAGGKYQAAGLTMITPSATQPDITDLGDFIWRIC
                     MTDAVQGKGLADYSVGTLGLQKVAIMYDNSDYGRGLADAYDEGVKAAGGSVVAKEQYA
                     TGDTDFKAQLTKIKGTGPELIFLSGYYPEGSKIAQQAKELGMDVQLLGSDGYASDELP
                     KLGGAAVEGMLVSTFFDYTKDDPAVQKFVDAYKAKYEGANPDWFVANSYDVIMLAAAA
                     AEKAGSNDRTAINDALAEIGTYEGISGPITFDENGDVIKPLSIVIVQDGSLVTAPQQP
                     AL"
     gene            3208..4146
                     /locus_tag="VFZ86_15660"
     CDS             3208..4146
                     /locus_tag="VFZ86_15660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020506023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX5643749.1"
                     /translation="MNEFAQQLVNGLMIGCLYALIAVGYTMVYGIIELINFAFGELFM
                     FGAFITIMMMSESISLFGATVGMPGLNFFVAILIAMAVVAVMGVVIERLAYRPLRNAP
                     RLAALISAIGVSILLQNVAQSIFGSQQVKFPDIPMFTTAAGAPKAFTVGNVEITYFMV
                     FVVVVTVITMLLLNVYVKRTRMGRAMRASAQDKNTSALMGVNVNKVIALTFLIGSAMG
                     ALAGGLYGGVYHAAWPLMGFNPGLKAFIAAVLGGIGNIPGAFLGGLLLGVIEALGSAY
                     LPMGSQYRDAFAFVILILILSFRPSGLLGSTVSQKA"
     gene            4148..5284
                     /locus_tag="VFZ86_15665"
     CDS             4148..5284
                     /locus_tag="VFZ86_15665"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX5643750.1"
                     /translation="MSDPTRNDDAAGPERPQTRLNPIASALTGDGTPRWQRALLLGLL
                     ALAIVLPFLVGSNRWITLMTVVVIYIVLASGLNIVVGFTGLLDLGYVAFYAIGAYYTA
                     IIFNQVLKVRFGVDIDDYWWLTWVNFLVGGLMAAVAGVLIGYPTLRVRGDYLAIMTLG
                     FGEIIRIVANNWVGLTRGPSGITGIPKPALFGYEFSTQLSLYFLGLAIALVSLWAISR
                     IVRSRIGRAWTAIREDEIAAEAMGIHTSRYKLLSYATGAFFAGAMGVFFGHYLNYISP
                     SSFMLMESFLILSLVVLGGMGNIAGPVLGATIWIGLQEWLRDFTFVQTHPETRGIAMG
                     LVLVLLMVFRPQGLLGSLRVKLEMRPGSKSEAEHERQVWSDAGH"
     gene            5271..6089
                     /locus_tag="VFZ86_15670"
     CDS             5271..6089
                     /locus_tag="VFZ86_15670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018657193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5643751.1"
                     /translation="MPATSDAAAVAAGAAAPSPRRVLETTQLVKAFGGLVAVNKVDFH
                     ASENEIVGLIGPNGAGKTTLFNAITGVYPPTSGAIRFEGRDIAGLLPSKVTKLGIART
                     FQNIRLFNEMTALENVMVGRHCRSHTNMLRVIARTPGAKRTEKAVRDASLELIDYVGL
                     SGHGGELSKNLPYGSQRRLEIARALATEPTLLFLDEPAAGMNPQETAALMGLIRRIRD
                     EGMTIILIEHDMKVVMGISDRVVVLDHGEKIAEGSPDEVRRDPRVIEAYLGKSA"
     gene            6086..>6229
                     /locus_tag="VFZ86_15675"
     CDS             6086..>6229
                     /locus_tag="VFZ86_15675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005149043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5643752.1"
                     /translation="MTADEAKGAPPGAAEVVLDVEDIRVFYGNIEALKGVSLQIRAGE
                     IVTL"
BASE COUNT          988 a         2248 c         2058 g          935 t
ORIGIN      
        1 gctccgcggc gaaccagccg ttgctgatga gcgtgacgaa gagcccggag cgtcgggcgg
       61 cgaggaggag ctccggcagg tcccgccgga gcaggggctc gccgccccac accaccagct
      121 gcgccatgcc ggcgcgccct gcctccgcgt acagccggcg cacggccgcg gtgccgagct
      181 cgcctgcagc cgcccgtccg tctccagcgg cccgctcacc ggcaggcgcg gcggtggcgc
      241 cgaccgccgc cgagccgccg gcgccggcac gccacaggca cgtggcgcag ctgccgttgc
      301 agcgggcggt gacgaggtgg ctgagcgtgt gcgggcgccc gcgggcgcgg gagacggcca
      361 tcctggccgt gagccgcgcc cgtgctctca ccctgtcgcg ggcgacgcgg ctgctgagta
      421 cgtcgtgcat gcacgagtgg taccacatcc gtgagcgcgc catgcccccc ggtttgcacc
      481 cggtgagccg gggaacgcct tccgtgtgac atcgactcac cgaaaggagg ctgacggcca
      541 atgtcagctg aactgaccgg caagcgcatc gcgatcctcg ccaccgacgg cgtggagggc
      601 gtcgagctca ccagcccgcg ggaggccctg gaacaggcgg gagccaccgt cgagctcctc
      661 gcgccggagg acggctccat ccagctgtgg gaccactccg agaagggcgg cacgctgccc
      721 gtcgacaggg cggtctcgga ggcggacccg cagacgtacg acggcctcgt gctgccgggg
      781 ggcgtacgca acgccgacca cctgcgcatg gacgccgcat ccgtgcgctt cgtagaagcc
      841 ctggctcgcc gagggacccc gatcggggtc atctgccacg gcgcctggat cctcatcgag
      901 gcgcaggccg tggacggccg caggctcacg tcgtacccct cgctgcggac cgacctgcag
      961 aacgccggcg cgcagtgggt cgaccaggaa gtcgtcaacg acgacggcct ggtctccagc
     1021 cggcgcccgg acgacctccc ggcgttcaac gccaagatcg tcgaggagtt cgccgagggc
     1081 gcgcacgggc gcagggcggc ctgacgccgc tgccgccgcg ggccgtgcgg cgaaagagat
     1141 cggcggcggg cggcgggcgc acgacgcccg ccgcccgcct tcactcgcgg agcactcggg
     1201 aggcccccat gaccgaacaa gaggtgttca tcctcgccga ccaggcgctc aacgccgtgg
     1261 tcgaccagat ccgtgacgac cagtgggacc tcgtggtccc cgacgacatg acgcgcactc
     1321 cggggatcac gctgcgccag accatcgact atcacgccta cgacgacgcc tgggtgccgc
     1381 acgtgctcgc gggggagacg atggaggacg tcggcgacag gtacgacggc gacctcctcg
     1441 gcgatcaccc gaagctgaac ttcgcgagca tcgcggagac ggcggtgctc gcggtccgcg
     1501 acttcaacga cccggaccgt atcgtccacc tcagctacgg cgactggccg gcgcgcgagt
     1561 acctcaagca catcaccagc ttccgaggcc tgcgcgcgta cgacatcgcg aagttcatcg
     1621 gtgcggacac gaccctgccg ccggacctgg tccggggcct gtgggacgag atcgcgccca
     1681 gcgcagagga gtggcgcaag ctcggcgtct acggtccggc ggtgccggtg ccggacgacg
     1741 ccccgctgca gcagaagctg ctcggactaa cgggccggca gcccggctga gccctcgggt
     1801 cgcggcagcc ggggcgacct tttccttgac tggtcgttca ccacccctct agactgcggc
     1861 gctagcgact gtcggcctgc tcgcaatgcg cggccgcagt tctcgcgctc gtacatccac
     1921 ccgggggagg aaatgtggac aagacctcaa gaaggctcgc tctcgcgctc atagcggtcc
     1981 tcgtgctgac gttcgcggcc gtggggctcg ctgcctgcgg cggcgacgag gccgcggacg
     2041 acggcgccgg cgagactgcg gcgccgttca agatcggcgt cgccggcccc atgacgggcc
     2101 agtacgccac ctacggcgcg tcccacaagg ccggcgccga gatcgccatg gaagagctca
     2161 acgccgacgg cggcgtcaac ggcggcgagg tctccatcgc cctcggcgac gacctgggcg
     2221 accccaagga ggccgtgctc gtcgcgcaga agttcatcga cgacaccgag ctcgtcttcg
     2281 tcaacggcca ccagttctcc ggcgcgacga tcgccgccgg cggcaagtac caggccgccg
     2341 ggctgacgat gatcacgccg tcggcgaccc agccggacat caccgacctg ggcgacttca
     2401 tctggcgcat ctgcatgacc gacgccgtcc agggcaaggg cctcgccgac tacagcgtcg
     2461 gcacgctcgg cctgcagaag gtcgccatca tgtacgacaa ctcggactac ggccgcggcc
     2521 tcgccgacgc ctacgacgag ggcgtcaaag ccgccggcgg ctccgtggtg gcgaaggagc
     2581 agtacgccac gggcgacacc gacttcaagg cccagctcac caagatcaag gggaccggcc
     2641 ccgagctgat cttcctctcc ggctactatc ccgagggctc gaagatcgcc cagcaggcca
     2701 aggaactcgg catggacgtg cagctcctcg gctccgacgg ctacgcctcg gacgagctgc
     2761 cgaagctcgg cggcgcggcg gtcgagggca tgctcgtgtc gaccttcttc gactacacca
     2821 aggacgaccc ggcggtgcag aagttcgtcg acgcatacaa ggccaagtac gagggcgcga
     2881 accccgactg gttcgtggcc aactcgtacg acgtgatcat gctggcggcc gcggcggccg
     2941 agaaggccgg ctcgaacgat cgcaccgcca tcaacgacgc cctcgccgag atcggcacct
     3001 acgagggcat ctccggcccg atcaccttcg acgagaacgg cgacgtcatc aagccgctca
     3061 gcatcgtcat cgtgcaggac ggcagcctcg tgacggctcc acagcagccg gcgctgtaag
     3121 cagaaacgca ggggggaggg cgggggacga ccccctcgcc ctccccgccc atccaccgac
     3181 cgcgacgcga gcgatgccga ggaccctgtg aacgaattcg cccagcaact ggtgaacggc
     3241 ctgatgatcg gctgcctgta cgccctcatc gccgtcggct acacgatggt gtacggcatc
     3301 atcgagctca tcaacttcgc cttcggcgag ctcttcatgt tcggcgcctt catcaccatc
     3361 atgatgatgt ccgaaagcat ctccctgttc ggcgccaccg tgggtatgcc ggggctcaac
     3421 ttcttcgtgg ccatcctcat cgccatggcg gtggtggcgg tcatgggcgt cgtcatagaa
     3481 cgcctcgcct accggcctct ccgcaacgcg ccccgcctgg cggcgctcat ctcggccatc
     3541 ggggtctcga tcctgctgca gaacgtcgcc cagtccatct tcggctccca gcaggtgaag
     3601 ttcccggaca tccccatgtt caccaccgct gcaggtgcgc ccaaggcgtt caccgtcggc
     3661 aacgtggaga tcacctactt catggtcttc gtggtggtcg tcaccgtgat cacgatgctg
     3721 ctgctcaacg tgtatgtgaa gcgcacgcgg atgggtcgcg ccatgcgcgc ctcggcgcag
     3781 gacaagaaca cgtccgcact catgggcgtc aacgtcaaca aggtcatcgc cctcaccttc
     3841 ctcatcggct cggcgatggg cgcgctggcc ggggggctgt acggcggcgt ctaccacgcc
     3901 gcgtggccgc tcatgggctt caaccccggc ctcaaggcgt tcatcgccgc ggtgctcggc
     3961 ggcatcggta acatccccgg cgcgttcctc ggcgggctgc tgctcggcgt catcgaggcg
     4021 ctcggctccg cctacctgcc gatgggctcg cagtaccgcg acgccttcgc cttcgtcatc
     4081 ctgatcctga tcctgtcgtt ccggccgtcc gggctgctcg gctccaccgt ctcgcagaag
     4141 gcgtgacatg agcgacccga cgcgcaacga cgatgcggca ggaccggagc gcccgcagac
     4201 acgcctgaac cccatcgcct cggccctgac cggcgacggc acgccgcgct ggcagcgcgc
     4261 gctgctcctg ggtctcctcg cgctggcgat cgtcctgccg ttcctcgtcg gctccaaccg
     4321 ctggatcacg ctgatgacgg tcgtcgtgat ctacatcgtg ctggcgtcgg gcctcaacat
     4381 cgtcgtcggc ttcacgggcc tgctcgacct cggctacgtc gccttctacg cgatcggcgc
     4441 gtactacacc gcgatcatct tcaaccaggt gctcaaggtg aggttcggcg tcgacatcga
     4501 cgactactgg tggctcacct gggtcaactt cctggtcggc ggcctgatgg cggcggtcgc
     4561 cggcgtgctg atcggctacc cgacgctgcg cgtccgcggc gactacctcg ccatcatgac
     4621 gctgggcttc ggcgagatca tccgcatcgt cgccaacaac tgggtcggcc tgacgcgcgg
     4681 cccctccggc atcaccggga tccccaagcc ggcgctcttc ggctacgagt tcagcacgca
     4741 gctcagcctc tacttcctcg ggctcgcgat cgccctggtc tcgctgtggg cgatctcgcg
     4801 gatcgtgcgc tcgcgcatcg gccgcgcctg gacggcgatc cgcgaggacg agatcgccgc
     4861 ggaggcgatg ggcatccaca cgtcgcgcta caagctcctc tcgtacgcga cgggcgcctt
     4921 cttcgccggc gccatgggcg tgttcttcgg ccactacctc aactacatct cgccgagcag
     4981 cttcatgctc atggagtcgt tcctcatcct gagcctcgtg gtcctcggcg gcatgggcaa
     5041 catcgccggg ccggtgctcg gggccaccat ctggatcggt ctgcaggagt ggctgcgcga
     5101 cttcaccttc gtccagacgc atcccgagac ccgcggtatc gccatgggtc tcgtcctggt
     5161 gctcctcatg gtcttccggc cgcagggcct gctcggctcg ctgcgcgtca agctggagat
     5221 gcggccgggg agcaagagcg aggccgagca cgagcggcag gtgtggtccg atgccggcca
     5281 ctagtgacgc ggcggcggtc gccgccgggg ccgccgctcc ctcccccagg cgcgtgctcg
     5341 agacgacgca gctcgtcaag gcgttcggcg gcctcgtcgc cgtcaacaag gtcgacttcc
     5401 acgcgagcga gaacgagatc gtcggcctca tcggccccaa cggagccggc aagacgacgc
     5461 tcttcaacgc catcaccggc gtctatccgc ccacgtccgg agccatccgc ttcgagggcc
     5521 gggacatcgc cggcctgctg ccgtccaagg tcaccaagct cggtatcgcg cgcaccttcc
     5581 agaacatccg cctgttcaac gagatgacgg cgctcgagaa cgtgatggtc ggccggcact
     5641 gccgatcgca caccaacatg ctccgcgtca tcgcgcgtac ccccggggcg aagcgcacgg
     5701 agaaggcggt ccgcgacgcg agcctggagc tgatcgacta cgtcggcctc tccgggcacg
     5761 gcggcgagct ctccaagaac ctgccctacg gctcgcagcg gcgtctcgag atcgcccgcg
     5821 cgctcgcgac ggagcccacg ctgctcttcc tcgacgagcc ggcggcgggg atgaacccgc
     5881 aggagaccgc cgccctcatg gggctgatcc ggcgcatccg tgacgagggc atgaccatca
     5941 tcctgatcga gcacgacatg aaggtggtca tgggcatctc cgaccgcgtc gtcgtgctcg
     6001 accacggcga gaagatcgcc gaagggtcgc cggacgaagt gcgtcgcgac ccgcgggtca
     6061 tcgaggcgta cctggggaag agcgcgtgac ggccgacgag gcgaagggcg cgccgcctgg
     6121 cgccgccgag gtcgttctcg acgtcgagga catccgcgtc ttctacggca acatcgaggc
     6181 gctcaagggc gtctcgctgc agatccgcgc cggcgagatc gtcacgctg
//
LOCUS       DASUMX010000332         4743 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_708461, whole genome shotgun sequence.
ACCESSION   DASUMX010000332 DASUMX010000000
VERSION     DASUMX010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 4743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4743
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708461"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="VFZ86_15680"
     CDS             complement(<1..525)
                     /locus_tag="VFZ86_15680"
                     /inference="COORDINATES: protein motif:HMM:NF014662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEX5643753.1"
                     /translation="MYEILDETLEQLEAGRAFALVTLVADQGSTPRAAGAEMLVRDDG
                     SIAGSIGGGLIEHTMMTAAATALAERRSRRERADLGGEDLASGEKMVCGGAADVLITF
                     VPPRDAELLAVCRALKDARDAGRRSWYVTVPPAGDDAGSFARPVEHCLLAESGELAGA
                     AVCEAATLRRLTAAA"
     gene            688..1986
                     /locus_tag="VFZ86_15685"
     CDS             688..1986
                     /locus_tag="VFZ86_15685"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013130441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEX5643754.1"
                     /translation="MPGTVVVGAQWGDEGKGKITDLLAEKADVVARFQGGNNAGHTIV
                     RDGEEFKFHLIPSGILYEAKTCVIGNGVVIDPHILFGELEALRGRGISGDNLRVSGDA
                     HLIMPYHLLLDGANEQQLGKYRIGTTGRGIGPCYVDKYTRIGIRVQDLLDEKILRRKV
                     KTALQEKNFLLQRYEIGVLDPDQVTDELLGYSERFEPFITDASLLVNTALDENKHVLF
                     EGAQGALLDIDFGSYPFVTSSNTIAGAACTGAGVGPTRIDHVIGVAKAYTTRVGEGPF
                     PTELFDEVGTAMRDIGHEYGTTTGRERRCGWLDLVALKYAVRLSGITHLAITKLDVLT
                     GMDTLKVCTRYKAGRRVLDEFPLLQTHFHHAKPLYEELPGWTEDLRDCETWQDLPQAA
                     RDYVQYIAEVTKTRVKFIAVGPGRDETIILPRSIGRGGAV"
     gene            2123..3124
                     /locus_tag="VFZ86_15690"
     CDS             2123..3124
                     /locus_tag="VFZ86_15690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643755.1"
                     /translation="MTGRLHSHKATAAAMLLALLAVLALLGVLALAPPVRAATGGAAG
                     PQQSVGNIVEVDQDVTVPSGTTADNVFVVGGSLTIAGTVRNVVAGIGADVRLLPSARV
                     GTAGGAGDTSLVVVGGTLTSAPGAAVVGDTTYEPLSAVRDVFTSGFWEPITTPFAGLS
                     LIGWAGSTVLLVLLGLLAAAVLPRQTRASEERIAGHFWSSLGWGALTALVIVPLITLA
                     LVVTIIGILVAIPWLFAAVALFLFGYLAFGAFFGRSLLRLVGYRGDSLMLAVTVGVIA
                     SQLVRLIPYVGAPIVFVMWTIGGGGAIAAFFVWRRSRRAGAVPTQGAEEEQKLRVAA"
     gene            complement(3181..4335)
                     /locus_tag="VFZ86_15695"
     CDS             complement(3181..4335)
                     /locus_tag="VFZ86_15695"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX5643756.1"
                     /translation="MTEDKTDSAVLVDEVGVELDELRRAGDQLDEAVALQFGLNRTDL
                     RCLGILYRRGRVTAGELAEESGLTPGAITTVLDRLERGGYANRVPDPADRRRVLVVST
                     VATREIGARIQGEVELASRRLLEGLETAELTCIRDFLRGMRSVYEAQIAALTAEGAPG
                     APSRESGVKVALAPEIGGGTDRPAQSSAPLGSASAGRLQFTKGAAKVSLKGDPALSDL
                     YQASFVGPVPEITVGGGTVVVQQRRRFRPFDWRAQSADFALSTAVPWDISLRGGMWKL
                     SADLTALRVTSLEVTGGASDIEIRLPAPTGTVPVRVSGGASKVTLLRPRGTEARAEVS
                     GGASSLAFDDQKLGAVGGRTVLASGGFADATDRYEIRFNGGASQVTVGTI"
     gene            4540..>4743
                     /locus_tag="VFZ86_15700"
     CDS             4540..>4743
                     /locus_tag="VFZ86_15700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015884027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5643757.1"
                     /translation="MDTAIEARGLVKTYRGDVRALDGVSLAAEPGSVFGLLGPNGAGK
                     STAVKILTTLSRPDAGEAFVAGHD"
BASE COUNT          665 a         1743 c         1668 g          667 t
ORIGIN      
        1 cgcggcggcc gtgagacggc gcagcgtcgc cgcctcgcag accgcggcgc cggcgagctc
       61 gccgctctcc gccagcaggc agtgctcgac ggggcgggcg aacgagccgg cgtcgtcgcc
      121 ggcgggcggc acggtcacgt accaggagcg gcgccccgcg tcgcgagcat ccttcagggc
      181 gcggcacacc gcgagaagct ccgcgtcccg cggcggaacg aaggtgatca gcacgtccgc
      241 cgcgccgccg cagaccatct tctcgccgct cgcgaggtcc tcgccgccga ggtccgcgcg
      301 ctcgcgccgc gaacgccgct cggcgagcgc cgtcgcggcg gcggtcatca tcgtgtgctc
      361 gatcagcccg ccgccgatgc tgccggcgat ggagccgtcg tcgcgcacca gcatctccgc
      421 gccggcggcg cgcggcgtgg agccctggtc ggcgaccagg gtgaccaggg cgaacgcgcg
      481 cccggcctcc agctgctcca gcgtctcgtc gaggatctcg tacatcgcac ctccgccacg
      541 attgtacagg gggcgtcccg ccgctatgat ttcgacaagg gacctgcctt cttttttcac
      601 cacccctcgg ccgccgctga gcctcgcacg tcgtgtcgcg agcccctccg gcggccgaag
      661 cagtcaagcg agacggaggg agacaccgtg cccggcaccg tcgttgtcgg cgcccagtgg
      721 ggcgacgagg gcaaaggcaa gatcaccgac ctgctggccg agaaggccga tgtggtcgcg
      781 cgcttccagg gcggcaacaa cgccggccac accatcgtcc gggacggcga ggagttcaag
      841 ttccacctca tcccctccgg catcctgtac gaggccaaga cctgcgtcat cggcaacggc
      901 gtcgtcatcg acccgcacat cctcttcggc gagctcgagg cgctgcgcgg acgcggcatc
      961 agcggcgaca acctgcgcgt gagcggcgac gcccacctca tcatgccgta ccacctgctg
     1021 ctggacggcg ccaacgagca gcagctcggc aagtaccgga tcggcaccac cggccgcggc
     1081 atcggcccat gctacgtgga caagtacacg cgcatcggca tccgcgtgca ggacctcctc
     1141 gacgagaaga tcctgcggcg caaggtcaag acggcgctgc aggagaagaa cttcctgctg
     1201 cagcgctacg agatcggcgt gctcgatccc gaccaggtga cggacgagct gctcggctac
     1261 agcgagcgct tcgagccgtt catcaccgac gccagcctgc tcgtcaacac ggcgctcgac
     1321 gagaacaagc acgtcctctt cgagggcgcc cagggcgcgc tgctcgacat cgacttcggc
     1381 agctacccgt tcgtcaccag cagcaacacg atcgccggcg cggcgtgcac cggcgccggc
     1441 gtcggcccca cgcgcatcga ccacgtcatc ggcgtcgcca aggcgtacac gacgcgcgtc
     1501 ggcgaggggc cgttccctac cgagctcttc gacgaggtgg ggaccgccat gcgcgacatc
     1561 ggccacgagt acggcacgac gacggggcgc gagcgccgct gcgggtggct cgacctcgtg
     1621 gcgctcaagt acgccgtgcg cctcagcggc atcacgcacc tcgcgatcac caaactcgac
     1681 gtcctcaccg gcatggacac gctcaaggtg tgcacccggt acaaggccgg gcgccgggtg
     1741 ctggacgagt tcccgctgct gcagacgcac ttccaccacg ccaagcccct ctacgaggag
     1801 ctccccggct ggaccgagga cctccgcgac tgcgagacct ggcaggacct cccgcaggcc
     1861 gcccgcgact acgtccagta catcgcggag gtcaccaaga cgcgggtgaa gttcatcgcc
     1921 gtcggccccg gccgcgacga gacgatcatc ctgccgcgca gcatcggccg cggcggcgcc
     1981 gtgtagcggc tcgcggcggg cccacgcctg cgccgcgggc aacggttacg ccttcgcggc
     2041 ggggggaagc cggactgcat ctgggcgggg atcgcgccca cgggcggggt ccgccgccga
     2101 atctccggca aggaggtgcg gcatgaccgg ccgcctccac tcacacaagg caaccgcggc
     2161 ggcgatgctc ctggcgctgc tggccgtcct cgcgctcctc ggtgtcctcg ccctcgcgcc
     2221 gcccgtacgg gccgcgaccg gcggcgccgc cggccctcag cagtcggtgg gcaacatcgt
     2281 ggaggtcgac caggacgtca cggtcccgag cggcaccacg gcggacaacg tcttcgtcgt
     2341 cggcggcagc ctgaccatcg ccggcaccgt gcgcaacgtc gtcgccggca tcggcgcgga
     2401 cgtgaggctg ctgccgtcgg cgcgcgtcgg tacggccggc ggcgccggcg acacctcact
     2461 cgtcgtcgtc ggcggcacgc tgacgtccgc accgggcgcc gccgtcgtcg gcgacaccac
     2521 ctacgagccg ctgtcggcgg tgcgggatgt gttcacctcc ggcttctggg agccgatcac
     2581 caccccgttc gcgggcctgt ccttgatcgg ctgggcgggg tccacggtgc tcctcgtcct
     2641 gctggggctg ctcgccgccg ccgtcctgcc gcggcagacg cgcgcgtccg aggagcggat
     2701 cgcgggtcac ttctggtcgt cgctcggctg gggggcgctc accgccctgg tgatcgtccc
     2761 gctgatcacg ctggcgctgg tcgtgacgat catcggcatc ctcgtggcga tcccctggct
     2821 gttcgccgcg gtggcgctgt tcctcttcgg gtacctcgcc ttcggcgcgt tcttcggcag
     2881 gtctctgctg cgcctcgtcg gctaccgcgg agacagcctg atgctcgcgg tcaccgtcgg
     2941 ggtcatcgcc tcgcagctgg tccggttgat cccgtacgta ggggcgccca tcgtcttcgt
     3001 gatgtggacg atcggcggcg gcggcgcgat cgcggcgttc ttcgtctgga ggcggagccg
     3061 gcgcgccggc gccgtcccga cgcagggcgc ggaggaagag cagaagctgc gcgtcgcggc
     3121 ctgacgggtg acgcgagcgc ggtgcggcgg ccggggcggg ccctccgccc cggccgccgc
     3181 tcagatggtc ccgaccgtca cctggctcgc gccgccgttg aagcggatct cgtagcggtc
     3241 ggtcgcgtcg gcgaacccgc cggaggccag gaccgtgcgg ccgccgaccg cgcccagctt
     3301 ctggtcgtcg aaggcgagtg agctcgcccc gccgctgacc tcggcccgag cctccgtgcc
     3361 tcgggggcgg agcagcgtga ccttgctggc gccgccactc acgcgcaccg gcaccgtgcc
     3421 cgtgggcgcg ggcagccgga tctcgatgtc gctggcgccc ccggtcacct ccaggctcgt
     3481 gacacgcagc gccgtgaggt cggcggagag cttccacatg ccgccccgca gagagatgtc
     3541 ccacggcacg gcggtcgaga gcgcgaagtc ggcggactgg gcgcgccagt cgaacggccg
     3601 gaagcggcgg cgctgctgca cgacgaccgt gccgccgccg acggtgatct cgggcacggg
     3661 tccgacgaag ctggcctgat acaggtcgct cagggccggg tcgcccttga ggctgacctt
     3721 cgcggcgccc ttggtgaact ggagccgtcc ggcggaggcg ctgcccagag gggcggagct
     3781 ctgcgccggt ctgtccgtgc cgccgccgat ctcgggcgcg agcgcgacct tgacgccgct
     3841 ctcgcgagaa ggcgcaccgg gggccccctc agctgtgagg gcggcgatct gggcctcgta
     3901 cacgctgcgc atccctcgca ggaagtcccg gatgcacgtg agctctgccg tctcaagacc
     3961 ctccagaagc cgccgcgacg cgagctccac ctcgccctgg attcgcgccc cgatctcgcg
     4021 cgtcgcgacg gtggagacca ccagcacgcg acgcctgtcc gccgggtcgg gcacccggtt
     4081 ggcgtacccg ccccgctcca gacggtcgag gaccgtggtg atcgcccccg gcgtcagacc
     4141 gctctcttcg gcgagctcgc cggcggtgac gcggccgcgc cggtacagga taccgaggca
     4201 gcggaggtcg gtgcggttga ggccgaactg gagcgccacg gcctcgtcca gctggtcgcc
     4261 ggcgcgccgc agttcgtcga gctcgacacc gacctcgtcg acgaggacgg cgctgtctgt
     4321 cttgtcttcg gtcatcacta cctcccggca ggacggggac acgcttgggg caggccgcaa
     4381 cggaggcctg cgggcggcga gcttggttcg ctgcggtgat gataccataa cctaatcatt
     4441 tgacaatcta attatcagcg ctacaatagc tccgcttgaa cgccagtcga ccggcgctcc
     4501 cgacacgggc ggcgccgagc gggaggaagg acgaggatga tggacactgc gatcgaggcg
     4561 cgcgggctgg tgaagaccta ccgcggcgac gtgcgcgccc tggacggcgt gagcctggcc
     4621 gccgagccgg gctccgtgtt cggcctgctg gggccgaacg gcgccggcaa gtcgacggcc
     4681 gtcaagatcc tgaccaccct gtcccggccg gacgccggtg aggcgttcgt ggccggtcac
     4741 gac
//
LOCUS       DASUMX010000333         3583 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1281447, whole genome shotgun sequence.
ACCESSION   DASUMX010000333 DASUMX010000000
VERSION     DASUMX010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3583
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1281447"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="VFZ86_15705"
     CDS             complement(<1..315)
                     /locus_tag="VFZ86_15705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643758.1"
                     /translation="MTSVVSQAAEVGPARDAAAAPRALPRSGFAWALLSGVFWGADGV
                     VLGVALAMTPFIGAAALVAPLAMAALHDGLSTGWMLAFNAATGRLRQLPRSLRSRHGL
                     VLV"
     gene            complement(312..1238)
                     /locus_tag="VFZ86_15710"
     CDS             complement(312..1238)
                     /locus_tag="VFZ86_15710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HEX5643759.1"
                     /translation="MEQPGVSGRVFDVQRFSLHDGPGIRTVVFLKGCPLRCAWCANPE
                     SQRAAPEIAWFENLCALCGRCVEACPHGVVCIDEGRVRTDRRLCAACGACAAACSRGA
                     RRLLGRESTVDEVMAEVRRDAPFFRRSGGGVTFSGGEPLSQPEFLVECLRRCRRWGYH
                     TAVETCGQARWEDVAAVAESADLFLYDLKQVDTARHEELTGAGNELILANLERLLGLG
                     AAVTVRVPVIPGANDDRESLAALADFVACHPGVRSVELLPYHPLGAHKYDALDLAWAA
                     FAKPEPAQLEEAAAYVAGLARGVDCVVTQGLG"
     gene            complement(1302..>3583)
                     /locus_tag="VFZ86_15715"
     CDS             complement(1302..>3583)
                     /locus_tag="VFZ86_15715"
                     /inference="COORDINATES: protein motif:HMM:TIGR01774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HEX5643760.1"
                     /translation="VFQETEGEPVVIRRAKGLKRTCETKTILIQDDELIVGNAGRRPR
                     TGVLGPEMSNFWFDKEMDTLSDRAQDPYQVDEDQKVLFREKVYPYWKGKTVIEHWLKR
                     VPEDTRALGYETGVIDVEIKTESGPGEIALGYGDILLPRGWGGVRRWAEERLAALDPA
                     DPASEEKGYFLQAVVISCEAMGILARRHAESARELATTSTPERAAELGRIADVCERIA
                     TEPPRTFHEALQLIWFAQMSLMMELNAPSYSPNRMDQYLYPYYEADVLAGDVSHDEAQ
                     ELLECLWVKLAEQCWILNTNCSQYFAGYTCFQNVTAGGVKGDGEDGTNAVSYMMLQAS
                     MDVRLNQPSLAVRLHRNCPEEFLLKVCELSRIGTGFPAIHNDEVGIKALLRKGVTTEE
                     ARDWCLVGCVEPNLPGKLHQWSDYGHYNFGSAVEFALFNGEHLLSGRKLGLETGDPND
                     FETFEEFEAAVMAQLAEQLRHIAIQGHVTEALHRELLPCALASALMLDCIDNGADLLR
                     GGARYKRGPGTLGIGLSDGANSLAAVRKLVYDEKRLTMDELREALRADFEGYEHVRDL
                     CLASPKYGNDDDDADRYVRHFAHLVVAEHDKYDTLTGGKMMPSWYPVSSNVPQGMTVA
                     ALPSGRRAGRPLADGVSPNQGTDRLGPTAVLRSVGKFQHEDLDGGTLLNVKFEPQVVA
                     GEDGLQRFAAYLRGFLDLPIYHVQFNVVKEETLRCAQAQPDEYRSLLVRVAGYSAFFV
                     ELHEDIQNDIISRTANAAF"
BASE COUNT          521 a         1252 c         1272 g          538 t
ORIGIN      
        1 gaccagcacg aggccgtgcc ggcttcgcag gctgcgtgga agctgcctca ggcgtccggt
       61 ggcggcgttg aaggccagca tccagccggt ggacaggccg tcgtgcagcg cggccatggc
      121 gagcggggcg acgagcgccg ccgcgccgat gaagggcgtc atcgccaggg ccacgccgag
      181 gacgacgccg tcggcgcccc agaagacccc ggacagcagc gcccacgcga agccggaccg
      241 cggcagcgcc cggggcgcgg ccgcggcgtc tcgtgcgggc ccgacctccg cggcctggga
      301 gaccacggac gtcatcccag cccctgcgtg accacgcagt ccacgccgcg cgcgagcccg
      361 gccacgtagg cggctgcttc ctcgagctga gccggctcgg gctttgcgaa cgccgcccac
      421 gccaggtcca gggcgtcgta cttgtgcgcc ccgagaggat ggtacggcag caactcgacg
      481 ctccgaacac ccgggtggca ggcgacgaag tccgcgagcg cggccaggct ctcgcggtcg
      541 tcgttggcgc cggggatcac cggcacgcgg acggtcacgg cggcgcccag gccgagcagc
      601 cgctccaggt tcgcgaggat cagctcgttg cccgcaccgg tgagctcttc gtgcctggcc
      661 gtatccacct gcttgaggtc gtagaggaac aggtccgcgg actcggccac ggcggccacg
      721 tcctcccagc gcgcctgccc gcaggtctcg acggcggtgt ggtagcccca gcgacgacag
      781 cgccgcaggc attcgaccag gaactccggc tgcgaaagcg gctcgccacc ggagaaggtg
      841 acgcccccgc ccgagcggcg gaagaagggg gcgtcccggc gcacctcggc catcacctcg
      901 tcgacggtcg actcacggcc caggaggcgc cgcgcccctc gcgagcacgc ggcggcgcac
      961 gcgccgcagg ccgcgcagag acgcctgtcc gtgcgcacgc ggccttcgtc gatacataca
     1021 acgccgtgcg gacaggcttc gacgcagcgg ccgcagagcg cgcagaggtt ctcgaaccag
     1081 gcgatctccg gggccgcccg ctgcgactcc gggttggcgc accaggcgca gcgcagcggg
     1141 cagcccttga ggaagaccac ggtgcggatg ccggggccgt cgtgcagcga gaagcgctgc
     1201 acgtcgaaca cccggccgct cacgcccggc tgctccacgc ggcctcccgt cgattcccgc
     1261 cgcaggctcc gaacggagcg gtgactcgaa aggctcaccg ctcagaaggc ggcgttggcc
     1321 gtgcgcgaga tgatgtcgtt ctggatgtcc tcgtgcagct ccacgaagaa ggcggagtag
     1381 ccggcgacgc gcaccagcag cgagcggtac tcgtccggct gggcctgcgc gcagcgcagc
     1441 gtctcctctt tcaccacgtt gaactggacg tgatagatgg gcaggtcgag gaagccgcgc
     1501 aagtaggcgg cgaagcgctg cagcccgtcc tccccggcca cgacctgcgg ctcgaacttg
     1561 acgttgagca gcgtgccgcc gtcgaggtcc tcgtgctgga acttgccgac ggagcgcagc
     1621 acggcggtcg gcccgagccg gtcggtcccc tggttgggcg agacgccgtc ggccagaggt
     1681 ctgccggccc tgcgcccact cggcagcgcc gccaccgtca tgccctgggg gacgttggag
     1741 gagaccgggt accaggacgg catcatcttg ccgccggtga gcgtgtcgta cttgtcgtgc
     1801 tcggcgacca cgaggtgggc gaagtggcgc acgtagcggt ccgcgtcgtc gtcgtcgttg
     1861 ccgtacttgg gcgacgcgag gcagaggtcg cgcacgtgct cgtagccctc gaagtcggcg
     1921 cgcagcgcct cgcggagctc gtccatcgtc aggcgcttct cgtcgtagac gagcttcctg
     1981 acggcggcca gggagttggc gccgtcggag agcccgatgc cgagcgtgcc ggggccgcgc
     2041 ttgtaccggg cgccgccgcg cagcaggtcg gcgccgttgt cgatgcagtc gagcatcagc
     2101 gccgacgcca gcgcgcacgg cagaagctcg cggtgcagcg cctcggtcac gtgcccctgg
     2161 atggcgatgt ggcggagttg ctcggcgagc tgggccatca ccgccgcctc gaactcctcg
     2221 aaggtctcga agtcgttcgg gtcgccggtc tcgagaccca gcttgcggcc gctcaggagg
     2281 tgctcgccgt tgaacagcgc gaactcgacc gcggagccga agttgtagtg gccgtagtcg
     2341 ctccactggt ggagcttgcc cggcaggttg ggctcgacgc agccgacgag acaccagtcg
     2401 cgcgcctcct cggtggtcac gcccttgcgc aggagcgcct tgatcccgac ctcgtcgttg
     2461 tggatcgcgg ggaagccggt gccgatgcgt gagagctcgc agaccttgag caggaactcc
     2521 tccgggcagt tgcggtgcag gcgcacggcc agcgagggct ggttgaggcg cacgtccatc
     2581 gacgcctgca gcatcatgta gctgacggcg ttggtcccgt cctcgccgtc gcccttgacc
     2641 ccgccggcgg tgacgttctg gaagcaggtg tagccggcga agtactgcga gcagttggtg
     2701 ttgaggatcc agcactgctc ggcgagcttg acccacaggc actcgagcag ttcctgcgct
     2761 tcgtcgtgcg acacgtcgcc ggcgaggacg tcggcctcgt agtaggggta gaggtactgg
     2821 tccatgcggt tgggcgagta cgagggcgcg ttcagctcca tcatcaggct catctgcgcg
     2881 aaccagatga gctgcagcgc ctcgtggaag gtgcgtggcg gctcggtggc gatccgctcg
     2941 cagacgtccg cgatgcgccc gagctcggcg gcgcgctcgg gcgtcgaggt cgtcgccagc
     3001 tcccgcgccg actccgcgtg ccggcgggcc aggatgccca tggcctcaca gctgatgacc
     3061 accgcctgca ggaagtagcc tttctcctcg ctcgcggggt cggccgggtc gagcgccgcg
     3121 agccgctcct ccgcccagcg gcgcacgccg ccccagcccc tgggcagcag gatgtcaccg
     3181 tagccgaggg cgatctcgcc cgggcccgac tcggtcttga tctcgacgtc gatcacgccc
     3241 gtctcgtacc ccaacgcgcg agtgtcttcg ggcacgcgct tcagccagtg ctcgatgacc
     3301 gtcttgccct tccaataggg gtagaccttc tcgcggaaga ggaccttctg gtcttcgtcc
     3361 acctggtacg ggtcctgcgc gcgatcggag agcgtgtcca tctccttgtc gaaccagaag
     3421 ttcgacatct ccggcccgag gacgcccgtg cgcggcctgc gcccggcgtt gcccacgatc
     3481 agctcgtcgt cctggatgag gatcgtcttg gtctcgcagg tgcgcttgag acctttggcc
     3541 ctgcggatga cgaccggctc tccctccgtc tcctggaaga cct
//
LOCUS       DASUMX010000334         3750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1426661, whole genome shotgun sequence.
ACCESSION   DASUMX010000334 DASUMX010000000
VERSION     DASUMX010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3750
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1426661"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="VFZ86_15720"
     CDS             complement(<1..366)
                     /locus_tag="VFZ86_15720"
                     /inference="COORDINATES: protein motif:HMM:NF013213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX5643761.1"
                     /translation="MSLVDAHCHLEMIADTAGAVAEARASGVGQIVTIGIDLETSRGA
                     AELSESLPGVYATIGLHPHDAHRLDDATLAAFEELAARPRVVAVGEVGLDFYRDLSPR
                     DAQRAAFRAQIALARRVGLP"
     gene            complement(414..1943)
                     /gene="metG"
                     /locus_tag="VFZ86_15725"
     CDS             complement(414..1943)
                     /gene="metG"
                     /locus_tag="VFZ86_15725"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF008900.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEX5643762.1"
                     /translation="MSTYITTPIYYVNSEPHHGHAYTTIAADVLARHHRQRGEDVFFL
                     TGVDEHGTKIAQAAEERGLTPQQHVDVMAPKYRALAAAVGASNDFFIRTTDPEHEAFV
                     QGFVARLQAAGDIEKRTYGGLYCTACEGFWYERDLTAEGMCPQHGIKPVWLEEENYYF
                     LLSRYQDRLAEFFRANPGFVTPRSRYNEALSFIEQGLEDISISRSSITWGIPVPWDQD
                     QVIYVWVDALINYLSALTYARPGEDLTARYWPAFHFLAKDILKFHAVIWPALLMSAGY
                     DLPTGELIHGYLLVGGEKMSKTRGNTLDPFAVIEEIGAEPLRYYLMREVTLGQDGDVS
                     LEGLRARYNNELANELGNLLSRTASMVVKYRGGDIPQAGPEGEALAAVAAEGEAMVGK
                     SAEQYAELNVTAALDTVWEYVRRLNRLVEEEAPWKLAKDETLAGRLDAVLNGLAAGLR
                     LVALAVYPVIPLTSVEVLRRLGQPCGDADLLLEKASWSALTPAAVVAGPPLFPRLETA
                     G"
     gene            complement(1954..2523)
                     /locus_tag="VFZ86_15730"
     CDS             complement(1954..2523)
                     /locus_tag="VFZ86_15730"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEX5643763.1"
                     /translation="MKIERKPSTALLPTPVVLLSVAGHGKEKPNIITLAWAGTVCSAP
                     PMLSVAIRPSRHSHGLVNAAREFVVNVPRAAQLEQVDLAGVWSGAEHDKFEELGLTAT
                     PASRVAAPLIEECPVNIECVVKHQLELGAHDLYIAEIVAVQYDEDVLDARGRVRTKKL
                     TPIAYVEGQYWSLGEQIGSYGAAAKASGR"
     gene            2633..3106
                     /locus_tag="VFZ86_15735"
     CDS             2633..3106
                     /locus_tag="VFZ86_15735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009082329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX5643764.1"
                     /translation="MRRQSLDKTDIAIVGHLQSDGRRPFTAIAKDLGISEASVRQRVA
                     RLLRSNVIQIVAVSSPLDLGLMWAQVHLRVRGDRIEAVAEAVAELAQVDYVSICAGDV
                     DIIVGVVARDRDELHDVLVNHIRTIPGIEQADFLLHLKILKDNYEWSPGEPPATV"
     gene            3120..>3750
                     /locus_tag="VFZ86_15740"
     CDS             3120..>3750
                     /locus_tag="VFZ86_15740"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5643765.1"
                     /translation="MGDQTRPHVHTPPPGPNSQALMKRGEDIMLGVSLIGAWPPSHFV
                     SRYRDGWFLEDVDGNRYIDWLAGWASSPLGGNHPELVEAAHGALKEYGTECLSSLTTW
                     HQYQLAEKLVEIAPKPLCKVVYDTTGSEVVENAVRIMREAAGPGRPFVITFLGSFHGG
                     NYGTGAMGPHNAHYNHGIEPFMSGWINVPFPTCYRCPYHLDYPACDIACL"
BASE COUNT          568 a         1317 c         1294 g          571 t
ORIGIN      
        1 cggcagcccc acgcgccgcg cgagcgcgat ctgggcgcgg aacgcggcgc gctgcgcgtc
       61 gcgcggagag aggtcgcggt agaagtccag gccgacctca ccgaccgcga ccaccctggg
      121 ccgcgccgcg agctcctcga acgccgccag cgtggcgtcg tccagccggt gggcgtcgtg
      181 ggggtgcagg ccgatggtgg cgtacacgcc cggcagagac tcggacagct ccgccgcccc
      241 gcgcgacgtc tcgaggtcga tgccgatggt gacgatctga cccacgccgg aggcccgcgc
      301 ctcggcgacc gcgccggcgg tgtcggcgat catctcgagg tgacagtgcg cgtcgacgag
      361 gctcatgggc gagtgtccgg cgcgggggcg gaccgtgcgg tcacgccggc gcctcagccg
      421 gcggtctcga gccgcgggaa cagcggcgga ccggcgacca cggccgcggg cgtcagcgcg
      481 ctccaggagg ccttctcgag caggaggtcg gcgtcaccgc acggctgccc gagccgcctg
      541 agcacctcga ccgacgtcag cgggatgacg ggatacacgg ccagcgcgac caggcgcagc
      601 cctgccgcca gcccgttgag cacggcgtcg aggcggcctg cgagggtctc gtccttggcg
      661 agcttccacg gcgcctcttc ttccacgagc cggttgaggc gccgcacgta ctcccagacg
      721 gtgtcgagcg cggcggtgac gttgagctcc gcgtactgct ccgcgctctt ccctaccatg
      781 gcctcaccct cagccgccac cgccgccaaa gcctcaccct caggtccagc ctgaggtatg
      841 tccccgcccc ggtacttgac gaccatgctc gccgtgcggc tgagcaggtt ccccaactcg
      901 ttggcgagct cgttgttgta gcgcgcccgc aggccctcga ggctcacgtc gccgtcctgt
      961 ccgagcgtga cctcgcgcat gaggtagtag cgcagcggct cggcgccgat ctcctcgatg
     1021 acggcgaacg ggtccagcgt gttgcctcgg gtcttgctca tcttctcgcc gccaaccagg
     1081 aggtagccgt ggatgagctc cccggtcggc aggtcgtagc cggcgctcat gagcagggcc
     1141 ggccagatga cggcgtggaa cttgaggatg tccttggcga ggaagtggaa cgccggccag
     1201 taacgcgccg tgaggtcctc cccggggcgc gcgtacgtca gcgcgctcag gtagttgatg
     1261 agcgcgtcga cccagacgta gatgacctgg tcctggtccc acgggacggg gatgccccag
     1321 gtgatgctgg agcggctgat gctgatgtcc tcgaggccct gctcgatgaa cgacagggcc
     1381 tcgttgtagc gcgaccgcgg cgtgacgaag cccgggttgg cgcggaagaa ctcggccagc
     1441 cggtcctgat accggctgag caggaagtag tagttctcct cctccagcca gaccggcttg
     1501 atgccgtgct gcgggcacat gccctcggcg gtgaggtcgc gctcgtacca aaagccctcg
     1561 caggcggtgc agtagaggcc gccgtacgtg cgtttctcga tgtccccggc ggcctggagc
     1621 ctcgccacga acccctgcac gaacgcctcg tgctcggggt cggtggtgcg gatgaagaag
     1681 tcgttggagg cgcccaccgc cgccgcgagc gcgcggtact tgggcgccat gacgtccacg
     1741 tgctgctgcg gcgtgaggcc gcgctcctcg gccgcctggg cgatcttggt gccgtgctcg
     1801 tcgacgccgg tgaggaagaa gacgtcctct ccgcgctggc ggtggtgccg cgccagcacg
     1861 tcggcggcga tggtggtgta ggcgtgcccg tggtgaggct cgctgttgac gtagtagatg
     1921 ggagtggtga tgtaggtgct cacggaggcc agtctacctt cccgaggcct tggcagccgc
     1981 cccgtaggag ccgatctgct cccccagcga ccagtactgc ccctccacgt aggcgatggg
     2041 cgtgagcttc ttcgtgcgca cgcgcccgcg cgcgtcgagg acgtcctcgt cgtactgcac
     2101 ggcgacgatc tcggcgatgt acaggtcgtg cgcgccgagc tcgagctggt gcttgacgac
     2161 gcactcgatg ttgacggggc actcctcgat gagcggcgcc gcgacgcgac tggccggcgt
     2221 ggcggtgagg ccgagctcct cgaacttgtc gtgctcggcc cccgaccaca cgccggccag
     2281 gtcgacctgc tcgagctgag cagcgcgcgg cacgttgacg acgaactcgc gcgccgcgtt
     2341 caccaggccg tgcgagtgcc gcgacgggcg gatggccacg ctcagcatgg gcggcgcgga
     2401 gcacaccgtg cccgcccacg cgagcgtgat gatgttgggc ttctccttgc cgtggccggc
     2461 gacgctcaac agcacgaccg gcgtcgggag cagcgcggtc gacggtttgc gctcgatctt
     2521 catcacaagc ctcccttggg tccggtcgta atggtacccc aagcgtggaa tcgcaacgtc
     2581 tctggcgccc aaatcacgac attcgtacaa tggggtggct gaaaggagtc tcatgcgcag
     2641 acagtccctg gacaagaccg acatcgccat cgtcgggcac ctgcagagcg acgggcggcg
     2701 gccgttcacc gccatcgcca aggacctcgg catctccgag gcgagcgtcc gccagcgggt
     2761 ggcccggctg ctgcgcagca acgtcatcca gatcgtcgcc gtcagcagcc cgctggacct
     2821 gggcctcatg tgggcgcagg tccacctgcg cgtgcggggc gaccgcatcg aggccgtggc
     2881 cgaggcggtc gccgagctcg cgcaggtgga ctacgtcagc atctgcgccg gcgacgtcga
     2941 catcatcgtc ggcgtggtcg cacgcgaccg cgacgagctg cacgacgtgc tcgtcaacca
     3001 catccgcacc atccccggca tcgagcaggc cgacttcctg ctgcacctca agatcctcaa
     3061 ggacaactac gagtggtcgc cgggcgagcc cccggcgacg gtatagggag gcgagagcca
     3121 tgggcgatca gacgcgcccg cacgtgcaca ccccaccccc aggcccgaac agccaggcgc
     3181 tcatgaagcg gggtgaggac atcatgctcg gcgtgtcgct catcggagcc tggccgccga
     3241 gccacttcgt ctcgcgctac cgggacggct ggttcctcga ggacgtggac ggcaaccgct
     3301 acatcgactg gctggccggg tgggcgtcgt ctcccctggg cggcaaccac cccgagctcg
     3361 tcgaggcggc ccacggggcg ctcaaggagt acggcaccga gtgcctctcg tcgctgacca
     3421 cctggcacca gtaccagctc gccgagaagc tggtggagat cgcgcccaag ccgctctgca
     3481 aggtggtgta cgacaccacc ggcagcgagg tcgtggagaa cgcggtgcgc atcatgcgcg
     3541 aggccgccgg gcccggccgc cccttcgtca tcaccttcct cggcagcttc cacggcggca
     3601 actacggcac cggcgccatg ggcccgcaca acgcccacta caaccacggc atcgagccgt
     3661 tcatgagcgg ctggatcaac gtccccttcc ccacctgcta ccgctgcccg taccacctcg
     3721 actatcccgc ctgcgacatc gcctgcctcg
//
LOCUS       DASUMX010000335         2317 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1571323, whole genome shotgun sequence.
ACCESSION   DASUMX010000335 DASUMX010000000
VERSION     DASUMX010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1571323"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            16..1032
                     /locus_tag="VFZ86_15745"
     CDS             16..1032
                     /locus_tag="VFZ86_15745"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX5643766.1"
                     /translation="MPVRLEGIHFLVTYRCTYACDHCFVWGSPDADGTMTLAQLTSVI
                     DQAADVGVTDVYFEGGEPTLAYPIVLAAAQRARERDLDVGIVSNCFWATSVADARVWL
                     APFADLGIADLSLSSYAYFVEDANEEQLRNAVLGAQELGIPVSVLEVGAPADIGVPGA
                     CSGDVGGVMHKGRAAVALAPARASRPPETLVTCPFEDFLDPGRAHIGPDGELQVCQGV
                     SAGNVFAGGDGPPAPEDLAAPRPDGLRAALDAYDPRARPVIRDILTGGPWALAQAAGH
                     TPARALYADECHLCYEVRAALRAAGRHPEVVAPGQCYAENEPAAGADAPAATPRPQEG
                     HHAV"
     gene            1022..1462
                     /locus_tag="VFZ86_15750"
     CDS             1022..1462
                     /locus_tag="VFZ86_15750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007195221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEX5643767.1"
                     /translation="MPSECERFWEAGSYAVVGHQASKKPYPKITYGALKERGKTVYPM
                     DPDGGTVEGDAAYSGFDALPGPVEAAVLELPKEETAAWVARAADAGVERIWIHQMTET
                     PEALAEAEKRGLKVITGHCAVMYNKPGMSMHAPHRWIWKLVGKY"
     gene            1589..>2317
                     /locus_tag="VFZ86_15755"
     CDS             1589..>2317
                     /locus_tag="VFZ86_15755"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HEX5643768.1"
                     /translation="MKRLVVVLLALSVLLIAAAPAAATPLSKAMSVDRPHASAVLYAP
                     RQAIPGERATKPYPSYTYYSDLGAILTRIDKRSARVSVKQFATSAGGHPLWLVTIQKK
                     WASPAAKQRWLKFIRLQTEDPTAALAMLRKGGDLRIPVFINNSIHGGETTGVDAGLML
                     IRKLAFSNDAVTRRILNNSVVMINACQNPDGRITDLRQNANGFDLNRDWIVQTQPEVR
                     AVAQQIVKWHPTLFLDTHGYYNPMI"
BASE COUNT          370 a          853 c          783 g          311 t
ORIGIN      
        1 gcgtacactg gcgccatgcc cgtccgtctc gaaggcatcc acttcctggt cacgtaccgg
       61 tgcacctacg cgtgcgacca ctgctttgtg tgggggtcgc cggacgcgga cgggacgatg
      121 acgctggccc agctcacgag cgtcatcgac caggcggcgg acgtgggcgt caccgacgtg
      181 tacttcgagg gcggcgagcc cacgctggcg tacccgatcg tgctcgcggc ggcgcagcgc
      241 gcccgcgagc gcgatctgga cgtcggcatc gtcagcaact gcttctgggc gacctcagtc
      301 gccgacgcca gggtgtggct ggcgccgttc gccgatctcg gcatcgccga tctctcgctc
      361 tcgtcctacg cctacttcgt cgaggacgcg aacgaggagc agttgcgcaa cgccgtgctc
      421 ggcgcgcagg agctcggcat cccggtgagc gtgctcgagg tcggggcgcc ggcggacatc
      481 ggcgtgcccg gcgcctgctc gggcgacgtc ggcggggtga tgcacaaggg ccgggcggcc
      541 gtcgcgctcg cgcccgcgcg agcgtcccgg ccgcccgaga cgctcgtcac ctgccccttc
      601 gaggacttcc tcgaccccgg ccgcgcgcac atcggccccg acggcgagct gcaggtgtgc
      661 cagggcgtca gcgccggcaa cgtgttcgcc ggaggcgacg ggcctccggc gcccgaagac
      721 cttgctgctc cgcgccccga cggcctgcgc gcggcgctcg acgcctacga cccgcgcgcg
      781 cgccccgtca tccgcgatat cctcaccggc ggcccgtggg ctctcgccca agccgccggc
      841 cacaccccgg cgcgcgcgct ctacgccgac gagtgccacc tctgctacga ggtgcgcgcc
      901 gcgctccgcg ccgccggacg ccacccggag gtcgtcgctc cgggtcagtg ctacgccgag
      961 aacgagccgg cagcaggcgc ggacgcgccc gctgccacgc cacgaccaca ggagggacac
     1021 catgccgtct gagtgcgaac gcttctggga ggccggcagc tacgccgtcg tcggccacca
     1081 ggccagcaag aagccgtacc ccaagatcac ctacggcgcc ctcaaggagc ggggcaagac
     1141 cgtgtacccg atggacccgg acggcggcac cgtcgagggc gacgccgcct acagcggctt
     1201 cgacgcgctg cccggcccgg tcgaggcggc cgtcctggag ctccccaagg aggagacggc
     1261 cgcctgggta gccagagccg cggacgccgg cgtcgagcgg atctggatcc accagatgac
     1321 cgagacgccg gaggcgctcg ccgaggccga gaagcgcggg ctcaaggtga tcaccggtca
     1381 ctgcgcggtg atgtacaaca agcccggcat gagcatgcac gccccgcacc gctggatctg
     1441 gaagctcgtc ggcaagtact gagccgggaa cccgggtcac gacgcgggcg ggctgcatac
     1501 tctgtatacc gcggggagta ttgtaggcgc cgcggctccc tgcgagactc ctgaggcaag
     1561 gtcggtcatc caactccgga ggggatccat gaagagactg gtcgtcgtcc tgctcgccct
     1621 gagcgtcctc ctcatcgccg cggccccggc ggcagccacg ccgctgtcca aggcgatgag
     1681 cgtcgatcgc ccgcatgcct cggccgtcct gtacgcgccg cgccaggcga ttcccggcga
     1741 gcgggccacc aagccgtatc cgagctacac ctattactcg gatctcgggg cgatcctgac
     1801 ccggatcgac aagcggagcg cccgggtcag cgtcaagcag ttcgccacct cggccggcgg
     1861 gcatccgctg tggctggtga ccatccagaa gaagtgggcc tcgcccgccg ccaagcagcg
     1921 ctggctcaag ttcatccgcc tccagacgga ggacccgacg gccgccctcg cgatgctgag
     1981 aaagggcggc gacctgcgca ttcccgtgtt catcaacaac agcatccacg gcggcgagac
     2041 caccggcgtc gacgccggcc tcatgctgat caggaagctg gcgttctcca acgacgccgt
     2101 gacgcggcgg atcctcaaca acagcgtcgt catgatcaac gcctgccaga acccggatgg
     2161 gcgcatcacc gacctgcggc agaacgccaa cggcttcgac ctcaaccgcg actggatcgt
     2221 gcagacgcag ccggaggtcc gggccgtggc gcagcagatc gtcaagtggc acccgacgct
     2281 gttcctcgac acgcacgggt actacaaccc gatgatc
//
LOCUS       DASUMX010000336         3846 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_710566, whole genome shotgun sequence.
ACCESSION   DASUMX010000336 DASUMX010000000
VERSION     DASUMX010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3846
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_710566"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1221
                     /locus_tag="VFZ86_15760"
     CDS             <1..1221
                     /locus_tag="VFZ86_15760"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX5643769.1"
                     /translation="AARAQALRAHAAFYRGDIFAARELIRDGHNQEGTIAQRAASLNT
                     HGMIDMALGDYDSALRHLSEAATAADGFGDTLTSHIEDNMAFLQASMGRTEAALHRLE
                     RLREHSDALDPTMLCSCLMHQGTVLRRLRDFEGALGPSQFATEIVGAERDPYLAYNAR
                     ANLALARGILEGGQRATLQELSALAAAAGVGFVELKCLLFGAVLAGSEDDPTASVSLL
                     EECLPRQLRFGHINLIAQELGPRPELAALILRRHRSNGLGPALVEALSHYWLFPETAE
                     TLRQLCPSQVGTWIDHMSAGNGRTGPRGTDLSHAPWPASPSHSSAGSSALNELTPRER
                     EVLELMAADRTNDEIAADLFIAIPTVKTHINHIRRKLDQTTRVGAVLEYQRLKGLVAE
                     GHEEQTRLHLHPPK"
     gene            1307..2326
                     /locus_tag="VFZ86_15765"
     CDS             1307..2326
                     /locus_tag="VFZ86_15765"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEX5643770.1"
                     /translation="MESSVSVTYVESAAMSQRKKTVIDILRDSNDFVSGEAISDVLGI
                     SRNAVHKHVKSLRKRGYRILGVSRRGYRLEDEPARLSMGHVTQRTENTMFGHSFRYHD
                     EIESTNLEAKALALKGAPEGTVVVAEAQTAGRGRLGRRWTSPAGKGLLFSVILRPALP
                     MSEAHLLTIVAATAAAEAVEKHVPARVAIKWPNDLFIADRKVGGILMEVSGERDEVDW
                     VVLGVGLNVNTEFSELPVALRRTATSLKIAGGEMVDRSDVLATLLLSLEAHYHAALRS
                     GFERALTAFRERDYLLARTISVETRQGPVIGAAAGIDDRGALLVELPHRRVRSFHAGD
                     VTLQP"
     gene            2412..2963
                     /locus_tag="VFZ86_15770"
     CDS             2412..2963
                     /locus_tag="VFZ86_15770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014406973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEX5643771.1"
                     /translation="MPRLTARELATAALFTAILAASAFVAIPVGSVPFTLQVYVVLLA
                     GMVLGPRLGVLSAVAYLVLGLVAPVYAGGTSGLGVLLGPTGGYLIGFILAALVSGLIA
                     GNRERPLGRLLIAGLGGLVPIYVLGPVWLAWQLDLTKDAAVLTGVLPFVWLDVMKAVA
                     AALTARVLVSLPLGLPVATRRDR"
     gene            complement(2917..>3846)
                     /locus_tag="VFZ86_15775"
     CDS             complement(2917..>3846)
                     /locus_tag="VFZ86_15775"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HEX5643772.1"
                     /translation="YRRAFRAFWRQEMPPFLRSLGVLLLLGLAIAALYAAAGIIAVAL
                     LFVPLFASQYMFKLLVREREHVTRQTELSDQYLEMNIGLAAAMVVLLDSKDEYTAQHS
                     AAVAMYCRDMANALDLPEEEAEALHLAGLLHDLGKVGVPDAVLRKANTLDEGEWEFIR
                     QHPEKGAEVLSHLAAYQEVADIVRFHHERLDGSGYPNGIHGDAIPELSKVLAVADSYH
                     AMTSDRPYRGARSSFEALKELRLMSGTTLDATYVEVLAKVLRDKDLAYRDGSSTDFMG
                     EYERGRINLRLRGHALADVSGLSGSRQEDPAAG"
BASE COUNT          649 a         1306 c         1243 g          648 t
ORIGIN      
        1 gccgcccgag cgcaagccct gcgcgcccat gccgctttct accgtggcga catctttgcc
       61 gcgcgcgaac tcatacgaga cggccacaac caagagggaa caatcgctca gcgcgctgca
      121 tcgctcaata cgcacggcat gatcgacatg gcgctcggcg actacgattc ggcgcttcgt
      181 cacctcagtg aggcggccac cgcagcagat ggtttcggcg acacgctgac gtcgcacatc
      241 gaggacaaca tggcattcct gcaggcgtcg atgggaagga cggaggccgc gctgcatcga
      301 ctagaacgac ttcgtgagca ttcagacgcc ctcgatccaa cgatgctctg ttcttgcctg
      361 atgcatcagg ggaccgtcct gcgcaggttg agggacttcg aaggagcgtt gggcccgtca
      421 cagttcgcga cagagatcgt gggcgcggaa cgcgacccct acctcgccta caatgcacga
      481 gccaatcttg ctttggcgag aggcattctc gagggagggc agcgcgccac cctccaagag
      541 ttgtcagcgc ttgcggcggc cgctggtgtg ggattcgttg agctgaaatg cctcctgttc
      601 ggagcggttc ttgcgggctc cgaggatgac cccactgctt ctgtcagcct gctggaggag
      661 tgcctaccaa ggcagctccg ctttggccac atcaacctaa tcgcgcagga gctcggtcct
      721 cgcccggagc tggccgcctt gatcctgcgc cggcaccgta gcaacggcct tggaccggcg
      781 ctcgtcgagg cgctgagtca ctactggctc tttcccgaga ctgcagagac attgcgccag
      841 ctctgcccat cacaggtggg aacgtggatc gaccacatga gcgccggcaa cgggaggact
      901 ggaccgcgcg gaactgacct ctcacacgcg ccttggccgg catctccaag ccactcatcc
      961 gccggctcct cagccctaaa cgagctcacc cccagggaac gagaagtgct cgaactgatg
     1021 gcggcggacc gcaccaacga cgagatcgcc gccgacctct tcatcgccat cccgaccgtg
     1081 aaaacgcaca tcaaccatat ccgtcgcaag ctcgatcaga ccacgcgcgt gggtgcagtc
     1141 ctcgagtacc agcgcctgaa gggtctggtc gccgaaggtc atgaggagca aactcgcctc
     1201 catctccacc cgccgaagtg aggaaatcaa ccctgagtat gattcccccc cgattccgca
     1261 cgactagcct cttcgcaccg tgcaccagca agcgaaagga agtcgaatgg agtcttcagt
     1321 ctccgtcacc tacgtcgagt ccgccgccat gagccagcgc aagaagaccg tcatcgacat
     1381 cctcagggac agcaacgact tcgtctcggg agaggccatc agcgacgtgc tcgggatctc
     1441 gcgcaacgct gtgcacaagc acgtcaagtc gctgcgcaag cgcggctacc gcatcctcgg
     1501 cgtgtcgcgg cgcggctacc ggttggagga cgagccggcg cgcctctcga tggggcacgt
     1561 cacgcaacgg accgagaaca cgatgttcgg gcactccttc cgctatcacg acgagatcga
     1621 gtccacgaac ctcgaggcca aagcgctcgc tctgaagggt gcgccggagg ggacggtcgt
     1681 agtggccgag gcacagacgg ccggccgcgg ccgcctgggc cggcgctgga cctcgccggc
     1741 cggcaagggg ctgctcttct ccgtgatcct gcgcccggcg ctgccgatga gcgaggcgca
     1801 ccttctcacc atcgtggcgg cgaccgcggc agccgaggcg gtcgagaagc acgtcccggc
     1861 ccgggtcgcg atcaagtggc cgaacgacct cttcatcgcg gaccgcaagg tgggaggcat
     1921 cctcatggag gtctcgggcg agcgggacga ggtggactgg gtggtgctcg gcgtcggact
     1981 caacgtgaac accgagttca gcgagctgcc ggtggccctc cggcgcaccg ccacctcgct
     2041 caagatcgcc ggcggcgaga tggtcgatcg cagcgatgtc ctcgccaccc tcctcctgag
     2101 cctcgaggcg cactaccacg ccgccctccg ctccgggttc gagagagcgc tcacggcctt
     2161 tcgcgagcgc gactacctcc tggcgcgcac catcagcgtg gagacccgcc aaggaccggt
     2221 gatcggggcg gcggcgggca tcgacgaccg tggagcgttg ctcgtggaac tgccgcaccg
     2281 acgcgtgcgc agcttccacg cgggtgacgt cacgcttcag ccctgacgcg tcaccggcct
     2341 cccgggccgc ttgacgaccg ctcgggaggc cggtacattg tcacctcttt ggtgctcaga
     2401 gaggttgacg attgccccga ctcaccgccc gcgagctcgc gacggccgcg ctcttcacgg
     2461 cgatcctcgc agcctccgcg ttcgttgcga tcccggtcgg ctccgttccc ttcacgctcc
     2521 aggtctacgt cgtgctgctc gcaggcatgg tgctcgggcc tcggctgggc gtgctcagcg
     2581 cggtggcgta cctggtcctc gggctggtcg cgccggtcta cgccggaggt acgtcgggct
     2641 tgggggtcct gctcggcccc acgggcgggt acctcatcgg cttcatcctt gcagccctcg
     2701 tctccggcct catcgccggc aaccgcgagc ggcctctggg acgactctta atcgctgggc
     2761 ttggtggcct cgtcccgatc tacgtgctgg gcccggtctg gctcgcgtgg cagctggatc
     2821 tgaccaaaga tgccgcggtc ctgacgggcg tcctgccgtt cgtgtggctg gacgtgatga
     2881 aggcggtggc agcagcgctc accgccaggg tactcgtcag cctgccgctg ggtcttcctg
     2941 tcgcgacccg gagagaccgc tgacgtcggc gagcgcgtgg ccgcgcagcc ggaggttgat
     3001 gcggccgcgc tcgtactcgc ccatgaagtc ggtggagctg ccgtcgcgat aggcgaggtc
     3061 cttgtccctg aggaccttgg cgagcacttc cacgtaggtc gcgtcgagcg tcgttcccga
     3121 catgagccgg agctctttga gcgcctcgaa ggagctgcgc gcgccgcgat acggccggtc
     3181 ggaggtcatc gcatggtagc tgtccgccac ggccaggacc ttgctcagct ccgggatagc
     3241 gtcgccgtgg atcccgttgg gatacccgct gccgtccaag cgctcgtgat ggaaacggac
     3301 gatgtccgcc acctcctggt acgctgcgag gtgggagagc acttcagcgc ccttctcggg
     3361 atgctggcgg atgaactccc actccccctc gtccagcgtg ttggccttgc gcagcaccgc
     3421 atccggcacg ccgaccttgc cgaggtcgtg gagaagcccc gccaggtgaa gcgcctcggc
     3481 ctcctcctcc gggagatcca gggcgttcgc catatcgcga cagtacatcg cgacggccgc
     3541 tgaatgctgt gccgtgtact cgtccttgct gtcgagaagc acgaccattg cggcggccaa
     3601 gccgatgttc atctcgaggt actgatcgct cagctcggtc tgccgagtga cgtgctcgcg
     3661 ctcgcgcacg aggagcttga acatgtactg gctggcgaac agcggcacga agagcagggc
     3721 gaccgcgatg atgccggcgg ccgcgtacag cgcagcgatc gccagtccaa gcagaagcag
     3781 cacgccgagg gagcgaagga acggcggcat ctcctgccgc cagaatgcgc ggaacgcgcg
     3841 acggta
//
LOCUS       DASUMX010000337         2112 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_2148491, whole genome shotgun sequence.
ACCESSION   DASUMX010000337 DASUMX010000000
VERSION     DASUMX010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2148491"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1500
                     /locus_tag="VFZ86_15780"
     CDS             <1..1500
                     /locus_tag="VFZ86_15780"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5643773.1"
                     /translation="VDFLGELEVTGRVHVGERVAVIGGGFTAMDACRTSLRKGAKEVT
                     CLYRRSRKEMPAHVTEVDEAEEEGVKLELLIAPVRVVTDERNKVTGIEMQRMELGEPD
                     ASGRRRPVPIEGSEFVIECDQVIAAVGQYPKLDGTSEEQGVKRTKWRTIAVDDWTFQT
                     EDPRVFAGGDAVLGAQTVIQAIAQGKKAAWSMDAYLRGEDMGAVAQSLAELRTTPFIT
                     ALNDRNDLDPRITRMAEIPPVFIDMTTDVTHPSPPAQMPKLGPEERKTNFRQIELGFA
                     EDEAVRGAELCLQCYCPANGKCDLQLHSIEYEVFKNRFHGGDAHDYPADFRHDFIMRE
                     PNRCINCGRCVRVCRMEVGSSCYDNMGRGFDTIVGTADNLPLQLVGCVSCGKCAETCP
                     TGSIETNPRILGSYDLDESRCIFCGECVEVCPYDALEQTDFFELAGYSRTTMSRESLY
                     VREARPVATLRETVKDLVPHVLDAERGQGWVWQPMKDDPIPLDDPEEQA"
     gene            1500..2012
                     /locus_tag="VFZ86_15785"
     CDS             1500..2012
                     /locus_tag="VFZ86_15785"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEX5643774.1"
                     /translation="MPGGDFHPWFFVIVAAWILICGLGVVLFRRIMYSAVSMVFCFLG
                     VAFAYALLNAPLVAIIQILVYVGAISIVIIFAIMLTEVQTGGMELFFNRQTAVAAVVA
                     LVGAAVLAVVALSADIGRIGDTSLQPGLRQLSRLIFDRYVFPFELVSLVLVAAMIGAI
                     VLATREKERP"
BASE COUNT          320 a          766 c          693 g          333 t
ORIGIN      
        1 gtcgacttcc tcggcgagct cgaggtcacc ggccgggtcc acgtcggtga gagggtcgcc
       61 gtcatcggcg gcggcttcac cgccatggac gcctgccgca ccagcctgcg caagggcgcc
      121 aaagaggtca cgtgcctcta ccgccgctcg cgcaaggaga tgccggcgca cgtcaccgag
      181 gtcgacgagg ccgaggagga gggcgtcaag ctggagctgc tcatcgctcc cgtgcgcgtg
      241 gtcaccgacg agcggaacaa ggtcaccggc atcgagatgc agcgcatgga gctcggcgag
      301 cccgacgcct ccggccgccg ccggccggtg cccatcgagg gctccgagtt cgtcatcgag
      361 tgcgaccagg tcatcgccgc cgtcggccag taccccaagc tcgacggcac cagcgaggag
      421 cagggcgtca agcgcaccaa gtggcgcacc atcgcggtcg acgactggac cttccagacg
      481 gaggacccgc gcgtgttcgc cggcggcgac gccgtgctcg gcgcgcagac cgtgatccag
      541 gcgatcgccc agggcaagaa ggccgcctgg agcatggacg cgtacctgcg cggcgaggac
      601 atgggcgcgg tcgcccagtc gctcgccgag ctcaggacga cgccgttcat cacggcgctc
      661 aacgaccgca acgacctcga cccgcgcatc acgcgcatgg ccgagatccc gccggtgttc
      721 atcgacatga ccaccgacgt cacgcacccg agcccgccgg cccagatgcc caagctcggt
      781 ccggaggagc gcaagacgaa cttccggcag atcgagctcg gcttcgccga ggacgaggcc
      841 gtccgcggcg ccgagctctg cctgcagtgc tactgcccgg ccaacggcaa gtgcgacctg
      901 cagctccaca gcatcgagta cgaggtgttc aagaaccgct tccacggcgg cgacgcccac
      961 gactacccgg ccgacttccg ccacgacttc atcatgcgcg agcccaaccg ctgcatcaat
     1021 tgcggccgct gcgtgcgcgt ctgccgtatg gaggtcgggt cgagctgcta cgacaacatg
     1081 ggccgcggct tcgacaccat cgtcggcacg gccgacaacc tgccgctgca gcttgtcggc
     1141 tgcgtcagct gcggcaagtg cgccgagacc tgcccgaccg gctccatcga gaccaacccg
     1201 cgcatcctcg gcagctacga cctcgacgag agccgctgca tcttctgcgg cgagtgcgtc
     1261 gaggtctgcc cctacgacgc gctcgagcag accgacttct tcgagctcgc cggctacagc
     1321 cgcaccacca tgtcgcgcga gtcgctctac gtgcgcgagg cgcgcccggt cgcgaccctg
     1381 cgcgagacgg tcaaggacct cgtgccgcac gtcctcgacg ccgagcgcgg gcagggctgg
     1441 gtctggcagc cgatgaagga cgatccgatc ccgctcgacg acccggagga gcaggcctga
     1501 tgcccggcgg cgacttccac ccctggttct tcgtcatcgt ggccgcgtgg atcctcatct
     1561 gcggcctcgg cgtggtgctg ttccgcagga tcatgtactc cgccgtctcg atggtgttct
     1621 gcttcctcgg ggtggcgttc gcctacgcgc tcctcaacgc gccgctggtc gcgatcatcc
     1681 agatcctggt gtacgtcggc gccatctcca tcgtcatcat cttcgcgatc atgctcaccg
     1741 aggtgcagac gggcggcatg gagctgttct tcaaccgcca gacggccgtc gccgcggtgg
     1801 tcgcgctggt cggcgccgcg gtcctggccg tcgtcgccct gtcggccgac atcggccgca
     1861 tcggcgacac gtcgctgcag ccggggctgc gccagctctc ccggctcatc ttcgaccgct
     1921 acgtgttccc gttcgagctc gtctcgctgg tgctcgtggc cgcgatgatc ggcgcgatcg
     1981 tgctcgcgac gcgcgagaag gagcgcccgt gaacgccctg tactggtggc tgctgctcgc
     2041 gttcctgctg ttctgcatcg gcgcgttcgg cgtgctcgcg cgccgcaacg tcatcctcgt
     2101 ggtgatgagc ct
//
LOCUS       DASUMX010000338         9428 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_565856, whole genome shotgun sequence.
ACCESSION   DASUMX010000338 DASUMX010000000
VERSION     DASUMX010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 9428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9428
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565856"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..372)
                     /locus_tag="VFZ86_15790"
                     /pseudo
     CDS             complement(85..372)
                     /locus_tag="VFZ86_15790"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(385..1338)
                     /locus_tag="VFZ86_15795"
     CDS             complement(385..1338)
                     /locus_tag="VFZ86_15795"
                     /inference="COORDINATES: protein motif:HMM:NF045333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 1"
                     /protein_id="HEX5643775.1"
                     /translation="MHEVNVGHKTLADYRSIVPKDLMTEIDELGERLKGRQVLHVNAT
                     AFGGGVAEILYTLVPLVSDVGLEAHWQILTAPPEFFNVTKSFHNGLQGHAVDFPPASR
                     ALYEDVCRANAAELTRPYDFVVIHDPQPLMIRDFVSLSQDRSTWWIWRCHIDTSTPDQ
                     ALYDYLVPSINRYDAAVYTLADYVPPGLTVPVREIAPTIDPLAPKNMQLSAEDATYIV
                     RQFGIDVERPLLLQVSRFDPWKDPLGVVDVYRAVKAKHPDVQLALIGSMASDDPEGWD
                     YLERVIAYVGGDPDVFILSNLDNVGSVEINAFQSHADVIMQ"
     gene            1405..2163
                     /locus_tag="VFZ86_15800"
     CDS             1405..2163
                     /locus_tag="VFZ86_15800"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5643776.1"
                     /translation="MAPSPSATPPPPEPVAFASDGLTLAGRLFRPAGPPQGALLVCHG
                     AGSRKENHELMGEQAAAAGLAALTFDFRGHGESDGAMGPDGWRDAVAAGERLLAWSGA
                     PWLAARGASMGGCMLLLAARARPGLFRSLVLLCPADGASLLAGLDELEDDPGAAPGGP
                     QGREYSGRFDAVALRPFLESLDLVAAARGLPRVLLAHARDDDAVPFAHSERLAAVLAP
                     PTRFIRLDEGGHHGPGRSADVARATIDWVLANGA"
     gene            complement(2182..4173)
                     /gene="feoB"
                     /locus_tag="VFZ86_15805"
     CDS             complement(2182..4173)
                     /gene="feoB"
                     /locus_tag="VFZ86_15805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HEX5643777.1"
                     /translation="MATAYHPRRHEVVDAHGADRPVVVCLAGNPNVGKSSLFNALTGE
                     SHETAHCAGVTVEACSVCARWDDRRIEVVDLPGSYALDGLGEDQLAARRAVLDWRPDV
                     VIAVADATNLARSLYLPLQLLDLGYRVVVALNLSDEARRRGRMPDAEVLSRDLGVPVV
                     ATVAPRGEGLADLEAAVLRVVAGGGLGETAGTPLPPDVEQRVEEVALHIAAQSDDGLH
                     APGGLGARAAALAVLEGDEDVIRAVGLTEGGFLTDADEGLALEISKARHAEARALAAA
                     AQDTRAAADRWWRLTTAPRTGVPILLGVIAAVFATLFIVGDLLARALTALWTAWISPL
                     VTDVVTAIFGDGTLGRVVLWGVDGGILATLAVGIPYILTFYFILALVEDTGYMNAAAF
                     LSDRVMHRIGLHGRAIIPLIAAGGCNVPAIIATRSLGNKRERTIAAVLATLTPCSART
                     AVIIGAVAGVAGWQYALLTYAVLIVVIILAGLGLNRLMPGDKGVLVMEMFPFRRPVLR
                     QVARKTWRRFREFVWDAAPIILLGSMALGLLYETGWVWELGTILEPIVEGWLLLPMVA
                     GLTLVFAVLRKELALQLLLVFAAVQAGGAVVSISSFMTTSQIVTYAIVNALYIPCVAT
                     IAVLGRELGWRRTALICTGTVVLALLVGGIVARVLPLFA"
     gene            complement(4179..4838)
                     /locus_tag="VFZ86_15810"
     CDS             complement(4179..4838)
                     /locus_tag="VFZ86_15810"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEX5643778.1"
                     /translation="MATRTQSVEEYLEAIFKLQRGRDPVTVKRLASELAVAPPSVSEM
                     IGRLRAAGLVEEPGEERGIALTEAGSAEGASLVRRHRLSERFLVDYLDMPWDTVHEEA
                     CKFEHVLSPEVEARLAEQLGNPLTCPHGHAIPGEDGSLADEELRPLSGLEPGEAAVIG
                     CIAEEKSDLLRYLASLGLLPDTPVSVESVAPFNGPLLVRVGGAQYALGREVTDKIMVR
                     G"
     gene            complement(4996..5071)
                     /locus_tag="VFZ86_15815"
     tRNA            complement(4996..5071)
                     /locus_tag="VFZ86_15815"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5036..5038),aa:Gly,seq:ccc)
     gene            complement(5137..5952)
                     /locus_tag="VFZ86_15820"
     CDS             complement(5137..5952)
                     /locus_tag="VFZ86_15820"
                     /inference="COORDINATES: protein motif:HMM:NF016816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HEX5643779.1"
                     /translation="MKLFIAIDLEGISGVVSEADTAREGAAARRARDHMRGDLDAVLE
                     GCAAAGASEVVVCDAHDDGRNLDPAGLPDHVTLIGGSPAPGSMLQGLAPDCDGALFVG
                     YHARAGTPAAVLEHTWSYKVFSASVGELEVGEFGLAALLAGHLGVPALYLCGDDKAAA
                     EALALVPGIATTVVKTGVRREAAEFVTPEAARARMRADVEAALRSSAWPPPLVWSGDP
                     LRLTFTRVTFCDLAEACPGARRLDGRTLEVGGSSFADVYAGFLACLRLSEAEG"
     gene            complement(5949..7031)
                     /locus_tag="VFZ86_15825"
     CDS             complement(5949..7031)
                     /locus_tag="VFZ86_15825"
                     /inference="COORDINATES: protein motif:HMM:NF017181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX5643780.1"
                     /translation="MTKHIDLLRDLCLAPGPTGFEAAAQEVFRRRLAPLGKASGDPLG
                     NVWLSVGPDDGPLVAVVAHADQIGLIVTYVDEHGYLSVERIGGVAPLLVPGRHFVVHG
                     RGGPVHAVGGRKPSHIIPEEERRTAPELHEQFLDIGAADRETALARVAVGDPVTFTPD
                     FLELSPGVVATQALDDRAGMYAVARALETYAAEPGSARLAGFATVHEETTFMGARALA
                     LRLRPDMLIVLDGDFCSDTPAADAKQLAGDVRLGRGPVLGRGAGSSEPLVALAREVAA
                     AEGIPVQVKAFPGETETDADELMAAGVCATLSVGLPLRYMHSPFEVACVDDLEAAARL
                     VAALARRAGEEGSAEDTAVTTPEVGA"
     gene            7191..7610
                     /locus_tag="VFZ86_15830"
     CDS             7191..7610
                     /locus_tag="VFZ86_15830"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HEX5643781.1"
                     /translation="MEEARPKWLRSTKGAPFTGIGSLEADILAVVWEKQVTTVRAVYE
                     TLRERRTIAYTTVMTVMNNLVKKHLLEQDKSAIAYVYKPAIPGREVAQTVLESVVHKL
                     IADQTGTAVSMLLGLDEDLTPAQLDELRAWVRQQYGG"
     gene            complement(7653..>9428)
                     /gene="argS"
                     /locus_tag="VFZ86_15835"
     CDS             complement(7653..>9428)
                     /gene="argS"
                     /locus_tag="VFZ86_15835"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEX5643782.1"
                     /translation="GGRTTRMTVTMSDDLYDLNGLAASLQAALEHELAAAGTSEIVAP
                     AVELSLPARPEHGDVTTNAALVTAKRAGRAPRELAEAVGARWKAGAGAGVCDRFEVAG
                     PGFLNLFLRDAWYRGALDRLLAEGGDYGRGALPADRRQKMNVEFVSVNPTGPLHVGHA
                     RYASYGDALCRILAFVGHDVTREFYVNDHGTQMTRFGQSLAARYGQRLGLDTPVPEEG
                     YQGEYLLVLADELIAAVGDRYREALAAVAPDVEALPAEVVGELRLWGRDAILAQFRVT
                     LERLRVPFDVWTPESSLYAGDGDHRGFGGEVGKSLADLDGESLLYDEGGACWLATTRF
                     GDDKDRVLIRQTGDPTYFLSDIAYHRDKMDRGFEHMIDIWGADHHGYVPRMKAAFTAL
                     PPHDPDKLELIIGQLVNLLESGESRRMSKRRGDIVTVDDLIDAIGVDAARFLLVGRSH
                     DTTLDLDIDLAVQQTNQNPVYYVQYAHARICSILRNLPAGGERPAGEAVPAVTVGPHE
                     RALLQALARFPDVLLSAADTRSPHRVHGYLGELAAEFHVFYRHCRVLSDDAAVAAFRA
                     GLCTAVRTTLAAGLGLLGVGAPESM"
BASE COUNT         1343 a         3341 c         3488 g         1256 t
ORIGIN      
        1 gacaccgggc cgcggtttgc ggccacgacg aggcggcgtt ccgcgagatc gacacgctcc
       61 acgccggccc tcctccgtcg ctggtcaggc tcctatctta ccttccatga ggtcggcgaa
      121 gaggcgcagg tcgtcgcgca gcaggcgcgg cgtgaggaac tcgcggcgca ccttctcctt
      181 gccgagcagc gccatctgcc ggtgcttctc cggctctgcc atgatctgca ggcagcgctc
      241 ggcgcactcg gcggccgagc tcaccaggta cccggtcacc ccgtcctcga tctggagcgg
      301 tatgccgccg acgtcgccgc cgacgaccgg ccggcccttc cacaggccct cggtgacggt
      361 gagcccgaag ccctcgcgcg tggactactg catgatcacg tcggcgtgac tctggaacgc
      421 gttgatctcc acggagccga cgttgtcgag gttggagagg atgaagacgt ccgggtcgcc
      481 gcccacgtag gcgatgacgc gctcgaggta gtcccagccc tccgggtcgt cgctggccat
      541 ggagccgatg agcgcgagct gcacgtccgg gtgcttcgcc ttgacggcgc ggtagacgtc
      601 gaccaccccc agcgggtcct tccaggggtc gaagcgggag acctgcagca ggagcgggcg
      661 ctccacgtcg atgccgaact ggcgcacgat gtaggtggcg tcctcggccg agagctgcat
      721 gttcttcggc gcgagcgggt cgatggtggg ggcgatctcg cgcacgggga cggtgaggcc
      781 cgggggcacg tagtcggcca gcgtgtagac ggccgcgtcg tagcggttga tcgacggcac
      841 caggtagtcg tagagcgcct ggtcgggcgt ggacgtgtcg atgtggcagc gccagatcca
      901 ccaggtggag cggtcctggc tgagcgagac gaagtcgcgg atcatgagcg gctgcgggtc
      961 gtggatgacg acgaagtcgt agggccgcgt gagctcggcg gcgttggcgc ggcagacgtc
     1021 ctcgtagagg gcgcgcgagg ccggcgggaa gtcgaccgcg tgcccctgca gcccgttgtg
     1081 gaagctcttg gtgacgttga agaactcggg gggcgccgtc aggatctgcc agtgcgcctc
     1141 cagcccgacg tcgctgacca gcggcaccag ggtgtacagg atctcggcca cgccgccgcc
     1201 gaaggcggtg gcgttgacgt gcaggacctg gcggcccttg agccgctcgc cgagctcgtc
     1261 gatctcggtc atcaggtcct tggggacgat gctccggtag tcggccagcg tcttgtggcc
     1321 gacgttcact tcgtgcatga gacctccgtc aaagggtggg agggtagtac atcacaccgc
     1381 tcccgttcgc taaactcggc cgcgatggcg ccatcccctt cagccacacc gccgccgccg
     1441 gaacccgtgg cgttcgcgtc ggatggcctc acgctggccg gtcgtctctt ccggcccgcc
     1501 ggcccgccgc agggcgcact gctcgtgtgc cacggcgccg gcagccgcaa ggagaaccac
     1561 gagctcatgg gtgagcaggc ggcagcggcg ggcctggccg cgctcacgtt cgacttccgc
     1621 ggccacgggg agagcgacgg cgccatgggg ccggacggct ggcgggacgc cgtcgccgca
     1681 ggcgaacggc tgctcgcgtg gtccggcgcg ccgtggctgg cggcgcgcgg ggccagcatg
     1741 gggggctgca tgctgctgct ggcggcgcgc gcccgtcccg gtctcttccg ctcgctggtg
     1801 ctgctctgcc cggcggacgg cgcatcgctc ctcgccgggc tggacgagct cgaggacgac
     1861 ccgggcgccg cgccgggcgg cccgcagggc cgcgagtact cgggccgctt cgacgccgtc
     1921 gccctgcggc cgttcctcga gagtctcgac ctggtcgccg cggcgcgcgg gctgccgcgc
     1981 gtgctcctgg cgcacgcgcg cgacgacgac gccgtgccgt tcgcccacag cgagcggctc
     2041 gccgccgtcc tggcgccgcc cacgcggttc atccggctgg acgagggcgg ccaccacggg
     2101 cccgggcgct cggcggacgt ggcgcgggcg acgatcgact gggtgctcgc gaacggcgcc
     2161 tgacggcgac tgagggcgcc gtcaggcgaa cagcggcagg acgcgggcga cgatgccgcc
     2221 cacgagcagc gcgagcacga cggtgccggt gcagatgagc gccgtgcgcc gccaccccag
     2281 ctcgcgcccc agcaccgcga tcgtcgccac gcacgggatg tagagggcgt tgacgatcgc
     2341 gtaggtgacg atctgcgagg tggtcatgaa cgaagagatg ctgaccaccg cgccgccggc
     2401 ctgcacggcg gcgaacacca gcagcagctg cagggcgagc tccttgcgca gcaccgcgaa
     2461 caccagcgtc agcccggcga ccatcggcag caagagccag ccctccacga tgggctcgag
     2521 gatggtgccc agctcccaca cccagccggt ctcgtagagc agcccgagcg ccatgctgcc
     2581 caggaggatg atgggggctg cgtcccagac gaactcgcgg aagcgccgcc acgtcttgcg
     2641 cgccacctga cggagcaccg ggcggcggaa ggggaacatc tccatgacca gcacgccctt
     2701 gtcgccgggc atcagccggt tcaagccgag gccggcaagg atgatgacga cgatgaggac
     2761 ggcgtaggtg agcagggcgt actgccagcc ggcgacgccg gcgacggcgc cgatgatgac
     2821 ggcggtgcgc gcgctgcagg gcgtgagcgt ggcgagcacg gcggcgatgg tgcgctcgcg
     2881 cttgttgccc agcgagcggg tcgcgatgat cgccggcacg ttgcagccgc cggcggcgat
     2941 gagcgggatg atcgcccgac cgtggagccc gatgcggtgc atgacgcggt cgctgaggaa
     3001 ggcggcggcg ttcatgtacc cggtgtcctc caccaatgcg aggatgaagt agaaggtgag
     3061 gatgtagggg atgccgacgg cgagcgtcgc caggatgccc ccgtccacgc cccagagcac
     3121 gaccctgccg agcgtgccgt cgccgaagat ggcggtcacg acgtcggtga cgagcgggga
     3181 gatccacgcc gtccagagcg ccgtgagcgc ccgcgcgagc aggtccccga cgatgaacag
     3241 cgtggcgaac acggcggcga tgacgccgag caggatgggg acgccggtgc gcggcgcggt
     3301 ggtgagccgc caccagcggt cggcggcggc gcgggtgtcc tgggccgccg ccgccagcgc
     3361 ccgcgcctcc gcgtgccgcg ccttggagat ctcgagcgcg aggccctcgt cggcgtcggt
     3421 gaggaagccg ccctcggtga ggccgacggc gcggatcacg tcctcgtcgc cctcgaggac
     3481 ggcgagggcg gccgcgcgcg cgccgagacc gccgggggcg tgcagcccat cgtcggactg
     3541 cgcggcgatg tgcagcgcca cctcctcgac gcgctgctcg acgtcgggcg gcagcggcgt
     3601 gccggcggtc tcgccgaggc cgccgccggc gacgacccgc agcacggcgg cctcgaggtc
     3661 ggcgaggcct tcgccgcgcg gcgccacggt ggccacaacg gggacgccga ggtcccgcga
     3721 gagcacctcg gcgtccggca tccgcccgcg ccgccgcgcc tcgtcggaga ggttgagcgc
     3781 caccacgacc cggtacccca ggtcgagcag ttgcagaggc aggtagaggc tgcgcgccag
     3841 gttggtcgcg tcggccaccg cgatgacgac gtccgggcgc cagtcgagca ccgcccgccg
     3901 cgccgccagc tggtcctcac ccagcccgtc gagcgcgtag ctccccggca ggtcgacgac
     3961 ctcgatgcgc cggtcgtccc agcgcgcgca gacggagcag gcctcgacgg tcacgccggc
     4021 gcagtgcgcg gtctcgtgcg actcgccggt gagcgcgttg aacagggagg atttgccgac
     4081 gttgggattg ccggccaggc agacgaccac gggccggtcc gcgccgtgcg cgtcgaccac
     4141 ctcgtggcgc ctggggtggt acgcggtggc cacggacgtc agccgcggac catgatcttg
     4201 tcggtcacct cgcggccgag ggcgtactga gcgccgccga cgcgcacgag cagcggcccg
     4261 ttgaacggcg cgacgctctc gacgctcacg ggcgtgtccg ggagcagccc gagcgacgcg
     4321 agatagcgca gcagatcgct cttctcctcc gcgatgcagc cgatgaccgc cgcctccccc
     4381 ggctcgagtc ccgagagcgg tcgcagttcc tcgtcggcga gcgagccgtc ctcgccgggg
     4441 atggcgtgcc catgggggca ggtgagcggg ttgccgagct gctcggcgag acgtgcctcc
     4501 acctcggggc tgagcacgtg ctcgaacttg caggcctcct cgtgcaccgt gtcccacggc
     4561 atgtcgagat agtcgaccag gaagcgctcg ctgaggcggt gccgccgcac gagcgacgcg
     4621 ccctcggccg acccggcctc ggtgagcgcg atgccgcgct cctcgccggg ctcctccacg
     4681 aggcctgcgg cccgcagacg gccgatcatc tcggagacgg acggcggggc cacggcgagc
     4741 tcggacgcga gtcgcttgac ggtgaccgga tcgcgcccgc gctgcagctt gaagatggcc
     4801 tcgaggtact cctcaacgct ctgcgtgcgt gtggccacgg tgcgactcca gtctgtgacg
     4861 gtcggcgggg cggatcctct gcgcaggcac gccccaggct cgtcattagg ataccctatg
     4921 cagcggcgtc agggcatcct gactttggtc aagaggacgt ccgacggacg gcgaccgggg
     4981 cacggaggat gggcttggag cggatgacgg ggctcgaacc cgcgatcttc agcttgggaa
     5041 gctgacgtgc taccagctgc accacatccg ctcgagggca ctatctgacc atagcggcgg
     5101 cgggcccgca agccgcgccc gcgccgcttc cggcgcctat ccctccgcct ccgagagccg
     5161 cagacaggcg aggaacccgg cgtagacatc ggcgaacgac gagccgccga cctcgagcgt
     5221 gcgcccgtcc agccgcctgg cgcccgggca cgcctcggcg aggtcgcaga acgtgacccg
     5281 cgtgaaggtc agccgcagcg ggtcaccgct ccagacgagc ggcggcggcc aggcgctcga
     5341 gcgcagcgcc gcctccacat cggcgcgcat gcgggcgcgc gccgcctccg gcgtgacgaa
     5401 ctccgccgcc tcgcggcgca cgcccgtctt gaccaccgtg gtggcgatcc ccggtacgag
     5461 cgccaaggcc tcggcggcgg ccttgtcgtc tccgcagagg tacagcgccg gcacgccgag
     5521 gtggccggcg agcagggcgg cgaggccgaa ctcgcccacc tcgagctcgc ccaccgaggc
     5581 cgagaacacc ttgtagctcc aggtgtgctc gagcacggcc gcgggcgtcc ccgccctggc
     5641 gtggtagccg acgaagaggg cgccgtcgca gtcgggagcc agcccctgga gcatgctgcc
     5701 gggcgccggc gacccgccga tgagcgtcac gtggtccggg agcccggccg ggtcgaggtt
     5761 gcggccgtcg tcgtgcgcgt cgcagacgac gacctcggac gcgccggccg cggcgcagcc
     5821 ctcgaggacg gcgtcgaggt cgccgcgcat gtggtcgcgg gcgcgccgcg ccgccgcgcc
     5881 ctcccgtgcc gtgtccgcct cggagacgac gccggagatg ccctcgagat cgatcgcgat
     5941 gaacagcttc atgcgcccac ctccggggtc gtcaccgccg tgtcttccgc agagccctct
     6001 tctccggccc ggcgcgccag cgcggccacc aggcgcgccg ccgcctccag gtcgtcgacg
     6061 cacgcgacct cgaacggcga gtgcatgtag cgcaggggca ggccgacgct gagcgtggca
     6121 cacacgccgg cggccatcag ctcgtcggca tcggtctcgg tctctccggg aaacgccttg
     6181 acctgcaccg ggatgccctc ggcggccgcg acctcgcggg cgagcgccac caggggctcg
     6241 ctgctgccgg cgccacgacc gagcaccggc ccgcgaccga ggcggacgtc accggcgagc
     6301 tgcttcgcgt ccgccgccgg ggtgtcgctg cagaagtccc cgtcgagcac gatgagcatg
     6361 tccggccgca gccgcagggc gagcgccctg gcgcccatga acgtggtctc ctcgtggacg
     6421 gtggcgaagc ccgcgagccg cgcggagccg ggctccgccg cgtacgtctc gagcgcgcgg
     6481 gcgacggcgt acatgccggc gcggtcgtcg agcgcctgcg tggcgaccac gccgggggac
     6541 agctcgagga agtccggcgt gaaggtcacg gggtcgccga ccgccacgcg cgcgagcgcg
     6601 gtctcccggt cggcggcgcc gatgtcgagg aactgctcgt gcagctccgg cgccgttcgg
     6661 cgctcctcct ccgggatgat gtggctcggc ttgcgcccgc ccaccgcgtg gacgggtccg
     6721 ccgcgaccgt ggaccacgaa gtgccgcccg ggcaccagca gcggcgccac gccgccgatc
     6781 cgctccacag acaggtaccc gtgctcgtcc acatacgtga cgatcaggcc gatctggtcg
     6841 gcgtgcgcga cgacggcgac cagcgggccg tcgtcgggac caacgctcag ccagacgttg
     6901 ccgagcgggt cgcccgaggc cttgccgagc ggcgcgaggc ggcggcggaa gacctcctgg
     6961 gccgccgcct cgaaccccgt ggggcccgga gcgagacaga gatctcgcag caaatcgatg
     7021 tgtttcgtca ctgcccctcc accccgacga tcgtcaccgc tgtcgggagg acatcagtct
     7081 acgccacggg cgtgagtttg tgctgaatgg gaatacgtac cttgtcatac gtactgcaca
     7141 gcgcggcggg cggcgcggcg tacgcacacg cgcacggcaa gggagtcgtg atggaggagg
     7201 caagaccgaa gtggctgcgc agcaccaaag gcgcaccctt caccggcatc ggttctctcg
     7261 aggccgacat cctggcggtc gtctgggaga agcaggtgac gacggtgcgc gccgtctacg
     7321 agacgctgcg cgagcggcgg acgatcgcct acacgaccgt catgacggtc atgaacaacc
     7381 tcgtgaagaa gcacctgctc gagcaggaca agtccgccat cgcctacgtc tacaagcccg
     7441 ccatccccgg ccgtgaggtg gcgcagacgg tgctcgagag cgtggtgcac aagctgatcg
     7501 ccgatcagac gggcaccgcg gtctccatgc tgctcgggct ggacgaggac ctcacgcccg
     7561 cgcagctcga cgagctgcgc gcctgggtgc ggcaacagta cggcggctga gcgcggaggg
     7621 acggccgcgc gcggccgtcc ccgccctcgg gctcacatcg attccggcgc gccgacgccg
     7681 agcaggccca gccccgcggc gagcgtggtc cgcaccgcgg tgcacagccc ggcgcggaac
     7741 gcagccacgg ccgcgtcgtc gctgagcacg cggcagtggc ggtagaacac gtggaactcc
     7801 gccgcgagct cccccagata cccgtgcacc cggtgcggcg agcgggtgtc cgcggcgctc
     7861 agcagcacgt ccgggaagcg cgcgagagcc tgcagcagcg cacgctcgtg cggccctacc
     7921 gtcacggccg ggacggcttc gcccgccggc cgctcccccc cggcgggcag gttgcgcagg
     7981 atgctgcaga tgcgcgcgtg cgcgtactgc acgtagtaca cggggttctg gttggtctgc
     8041 tgcacggcga ggtcgatgtc cagatccagc gtcgtgtcgt gagagcgccc gacgagcagg
     8101 aaccgcgccg cgtccacgcc gatggcgtcg atgaggtcgt ccaccgtgac gatgtcgccg
     8161 cggcgcttgc tcatccgccg cgactcaccg ctctcgagca gattgaccag ctgcccgatg
     8221 atgagctcca gcttgtccgg gtcgtgcggc ggcagcgccg tgaaggcggc cttcatgcgc
     8281 ggcacgtacc cgtggtggtc ggcgccccag atgtcgatca tgtgctcgaa cccgcggtcc
     8341 atcttgtcgc ggtggtaggc gatgtcgctg aggaagtagg tcgggtcgcc ggtctggcgg
     8401 atgaggaccc ggtccttgtc gtcgccgaac cgggtcgtgg cgagccagca ggcgcctccc
     8461 tcgtcgtaga ggagactctc tccgtcgagg tctgcgagtg acttgcccac ctcgccgccg
     8521 aacccgcgat ggtcgccgtc gccggcgtag aggctgctct ccggcgtcca cacgtcgaag
     8581 gggacgcgaa gacgctcgag ggtgacgcgg aactgggcga ggatggcgtc gcggccccag
     8641 agcctgagct cgccgaccac ctccgccggc agcgcctcga cgtccggggc gaccgccgcg
     8701 agcgcctcgc gatagcggtc gccgacggcg gcgatgagct cgtcggcgag gaccaggagg
     8761 tactcgccct ggtagccctc ttcgggcacc ggcgtgtcca ggccgagccg ctgcccgtag
     8821 cgggctgcga gcgactggcc gaagcgggtc atctgcgtgc cgtggtcgtt gacgtagaac
     8881 tcgcgggtga cgtcgtggcc gacgaaggcg aggatgcggc agagcgcatc cccgtacgac
     8941 gcgtagcgcg cgtggccgac gtgcagcggc ccggtcgggt tgacgctgac gaactcgacg
     9001 ttcatcttct ggcgccggtc cgccggcaac gcgccccggc cgtagtcgcc gccctcggcg
     9061 aggagccggt cgagagcgcc gcgataccag gcgtcgcgca ggaacaggtt gagaaagccg
     9121 ggcccggcga cctcgaagcg gtcgcagacg ccggcgccgg cgccggcctt ccagcgggcg
     9181 cccacggcct cggcgagctc gcgcggcgcg cgcccggcgc gtttggccgt gaccagcgcc
     9241 gcgttggtcg tgacgtcgcc gtgctcgggc cgcgccggca gcgagagctc cacggccgga
     9301 gcgacgatct cgctggtccc ggcggccgcc agttcgtgct cgagcgcggc ctgcagggag
     9361 gccgcgagtc cgttcaggtc gtagaggtcg tcgctcatgg tcaccgtcat ccgtgtcgtg
     9421 cgcccgcc
//
LOCUS       DASUMX010000339        11880 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_566047, whole genome shotgun sequence.
ACCESSION   DASUMX010000339 DASUMX010000000
VERSION     DASUMX010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 11880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11880
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566047"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..742
                     /locus_tag="VFZ86_15840"
     CDS             <1..742
                     /locus_tag="VFZ86_15840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005998767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5643783.1"
                     /translation="CPARARTHDTDVTVARENVLVVDDERDILELVKYNLDKEGYRVT
                     VVATGEDALAATRAKTPDLIVLDLMLPGVDGLEVCRRLKSDAKTRGIPIVMLTAKGSE
                     ADVVAGLELGASDYVTKPFSPRVLTARIRAVLRRGDETADDEATVHIKELTIHPGRHK
                     VLVGEKPVELTATEFRILLYLAKRPGWVFTRQQIVDAAQGDDSFVNDRFVTDRSVDVH
                     IVSLRRKLGCCGAYIETVRGVGYRLQDQ"
     gene            746..2542
                     /locus_tag="VFZ86_15845"
     CDS             746..2542
                     /locus_tag="VFZ86_15845"
                     /inference="COORDINATES: protein motif:HMM:NF013179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5643784.1"
                     /translation="MRRPRLFWRVFATYLVIVVLCTAAVGFYAVRSVRDFYVDHTERE
                     LQARAALVREQILPAILADTPEELEALVRRLGDSSGTRITLIAVDRPGYGRGEVLADS
                     DSDPVEMESHATRPEVLAALQGRPGRAIRYSRTLWQDMMYVAIPVTEDGRLTTVVRTA
                     VPLTRVNNALASLYGSIAVSAIVVAVIAAIIGLTVSRRISGQMRAIKVGAERLAAGDF
                     AHKLSVPRVEEFASVAESINRMAEQLDDKLRRLTHERNEREAVLASMVEGVLAVDIDE
                     RVIAVNAAAARLLDTDPASAEGKAIQEVVRNPDLQHVVAQTLDGHRPVEADIVIRVGA
                     EERNLQANGTLLHGDDDGDDVGAVVVLNDVTRLKRLEAVRRDFVANVSHELKTPVTSI
                     KGFAETLEDGALDDPAAARRFVRIIAGQADRLNSIIEDLLALSTLEQSGDSPLLQLEE
                     ADLCDVVAVALEVCGPKAEAKSIELREDCPGRLLARVSPPLLEQAVVNLIDNAVKYSS
                     EGSTVVVTLEERGDEVVVSVTDEGQGVAREHLPRLFERFYRVDKARSRDLGGTGLGLA
                     IVKHVAQIHGGRVSVDSLIGRGSTFRVHLPRG"
     gene            2623..3099
                     /locus_tag="VFZ86_15850"
     CDS             2623..3099
                     /locus_tag="VFZ86_15850"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF012470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HEX5643785.1"
                     /translation="MTKSALNVLDANRKGFFLMIEGGAVDWAGHANQPGRAIEEQIDF
                     NKAVEAVVRWVQRNSDWDETLVIVTGDHETGCLWGPGSGVPGGNGTWTPLVNNGEDVT
                     PGMQSNSGDHTNSLIPFFARGEGAADLRAAAMKADPIRGKYLDNTDIAKVILADMR"
     gene            3186..4598
                     /gene="pyk"
                     /locus_tag="VFZ86_15855"
     CDS             3186..4598
                     /gene="pyk"
                     /locus_tag="VFZ86_15855"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEX5643786.1"
                     /translation="MTDPQRRTKIVATLGPASRDEPVLARLLESGMDVARINLSHGTT
                     DEHAATIALLRRVAAERGVSLAILIDLPGPKLRTGDLPRPLTVRAGEVVTLGPSAAAQ
                     LPVNFPEMLPHLTEGELILVDDGAVALRVAAVSPTVVTLEALNDGAIGSRKGVNLPDT
                     QLPIAAFTGTDSDLLAWGLQQDIDYVALSFVRDAADILDLKRRIAAAGGDQLVVAKIE
                     RKEALGAYREIVAAADAVMVARGDLGVEVDAATVPVWQKRIIHAAVTAGRPVIIATQM
                     LQSMIASPRATRAEASDVANAIYDAASAVMLSGETAIGGYPVRAVETMREIALVVEAD
                     IEREGRAPRPWTEGRTGVSDAISHGACEVAERIGAAAIVTATFSGATARAVARSLPDQ
                     PIVAISPNQRVVNQLALCWGVHALLGGHRASFEEIVREANDLVLAGGFGLPGDSVVIT
                     AGLQTHQSGKTNLIKAHILA"
     gene            4936..5916
                     /locus_tag="VFZ86_15860"
     CDS             4936..5916
                     /locus_tag="VFZ86_15860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006129078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX5643787.1"
                     /translation="MRRIRWFAMVAAVMAIALVGVACGGDEPSDGASGDGTAALSGDI
                     RIDGSSTVGPLTEAAAEFFQEENPDVRVTVGISGTGGGFEKFCAGETDANDASRQIEA
                     DEEELCATAGVEWVEIQVANDGLAVVVNPENDWADSLTVEQLKMIWDEGSTIDNWNQV
                     DPSFPDVPLELFGAGTDSGTFDYFTEAVNGEEGVSRTDYQATEDDNVSVQGVAGSKGG
                     MAYFGLSYYEQNADQLKLVAIDNGNGPVTPSLETVQSGEYTPLSRPLFIYPSVESLQR
                     PEMAAFMQFYLDNAQSIAEQALFVPITAEQTAAAQAVLDGIVGGASPAAE"
     gene            5924..6871
                     /gene="pstC"
                     /locus_tag="VFZ86_15865"
     CDS             5924..6871
                     /gene="pstC"
                     /locus_tag="VFZ86_15865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006129080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEX5643788.1"
                     /translation="MAAVATERPASRPLEGLEAGSRRYREKVIVGSLFLCAALSVLVT
                     VGIVLALVIPSVEFFGTVSPVEFFTGTEWAPLFKNSKFGVLPLLSGTLVVTVIAALIC
                     LPLGLLAAIYLSEYAARRTRSFVKPILEILAGIPTVVYGFFALTFVTPLLRENWLFET
                     PPQVFNALSAGLVMGIMILPTVASISEDAMYAVPQSLRAGAAALGATKYEISTRVVVP
                     AGLSGIIAAFVLGISRAVGETMIVLIAAGGTPNLTLNPVEAMQTMTAFIGAAGLGDQS
                     TGSIGYKTIFAVGMTLFVMTLIMNIISIRLVRKYREVYD"
     gene            6868..7782
                     /gene="pstA"
                     /locus_tag="VFZ86_15870"
     CDS             6868..7782
                     /gene="pstA"
                     /locus_tag="VFZ86_15870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006456590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEX5643789.1"
                     /translation="MSTSTPQKSASSSAPISLSSRRGWRHRARDGAFRGAVTGSVVVG
                     LVVLVALILQSLIKGWPRLNLDLITSFPSSFPDRAGIQSAIFGTFWMVGIVLVVAIPI
                     GVGAAVYMEEFADSEAWYNRFIDLIIQNLAAVPSIVYGILGLAFLSRGLLNLGPVVIT
                     AGLTLSFLVLPVVIIASREAIRAVPSSILDGALALGATRWQGVSRQVLPASIPGIATG
                     SILAVSRAIGEAAPLLLLGALQFITFNPTGLDSSFTALPIQIFNWISRPQEGFHIAAA
                     AAIIVLLCITLLMNAVAIWLRNRFQQTW"
     gene            7994..8758
                     /gene="pstB"
                     /locus_tag="VFZ86_15875"
     CDS             7994..8758
                     /gene="pstB"
                     /locus_tag="VFZ86_15875"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017609105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEX5643790.1"
                     /translation="MRDVSVTYAGSYAVQGVDMSIRKNEITALIGPSGCGKTTVLRCL
                     NRTNDLIPRAKVEGKVLYHGIDLYGPKVEPVEVRRRIGMVFQKPNPFPKSIYDNVAFG
                     PRVGGFKGDMDELVERSLRRAALWDEVKRDLKASAMALSGGQQQRLCIARCIAVEPDV
                     ILMDEPCSALDPVSTLRIEELMYELVSEYTIVIVTHNMQQAARVSDRTGFFTVAESQD
                     EKDHRIGMLVEFDLTPTVFTNPSDSRTEAYVTGRFG"
     gene            complement(8852..9328)
                     /locus_tag="VFZ86_15880"
     CDS             complement(8852..9328)
                     /locus_tag="VFZ86_15880"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5643791.1"
                     /translation="MQLRHVRTSDCDRVVAVIDEWWGGPAAGSYLPHVFFSHLTSTSF
                     AFEASDGVLVGFLLGFLSQAHPDEACVHMIGVHPEFRRLGFGRRLCERFFVAAQMNGR
                     RYVRAVSPPSTQSVAFHLAMGFTQETTGTCGHRIVFRRGVVHDTQGVPGPCEALTA"
     gene            complement(9821..10525)
                     /gene="phoU"
                     /locus_tag="VFZ86_15885"
     CDS             complement(9821..10525)
                     /gene="phoU"
                     /locus_tag="VFZ86_15885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEX5643792.1"
                     /translation="MERETRTAFITQLEELERRALAGFDEVGEQLVSAIRSVRRGDAR
                     LAATVVAADRPINHRYLDIHHGAITLLASQAPVAGDLRLVAALLHVVWCLERMGDQCV
                     NIAKLVPLSGHESPRDEQMLAAIEDMGRLVGRQIMQAKETFVSRNVKLAEDLALQDTE
                     VDQLNRQVFRRAVDIGDDVELREWAMFMVLMARALERIGDNTVDVAEQTVFLVTGELR
                     ELVDVRHFSAEGRTPD"
     gene            complement(10746..10979)
                     /locus_tag="VFZ86_15890"
     CDS             complement(10746..10979)
                     /locus_tag="VFZ86_15890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643793.1"
                     /translation="MITRRMKIRRQLRSVRASALSEEERRQELREAEANGTHGHVRDE
                     PAGSAASPRAAGDDGAPRPPEAGAGRAGVSEED"
     gene            11224..>11880
                     /gene="phoU"
                     /locus_tag="VFZ86_15895"
     CDS             11224..>11880
                     /gene="phoU"
                     /locus_tag="VFZ86_15895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014812780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEX5643794.1"
                     /translation="MRHLQREIDRLKRKVLALGALVEENLRLAFQAIDQRDAAKARRV
                     IATDVLIDQNEVDVEEECLKILALYQPVAGDLRFVAAVIKINSELERIGDLSANIAER
                     ALQLVDEHPVAVPHNVAVMADRTWTILEKSLDALVRQDAVMAREVLVADDEVDALYAE
                     LIEELKAVIRADLEHLDAIVLLFSVARYLERLADHATNIAEDVLYMVEGEIQRHNQSA
                     V"
BASE COUNT         1949 a         4075 c         4025 g         1831 t
ORIGIN      
        1 ctgccctgcc cgcgcccgca ctcacgacac ggatgtgacg gtggccagag agaacgtgct
       61 cgtcgtcgac gacgagcggg acatcctcga gctcgtcaag tacaacctcg acaaggaagg
      121 ctaccgagtg acggtggtgg ccaccggtga ggacgccttg gcggcgacgc gcgccaagac
      181 accggacctc atcgtgctcg acctgatgct gcccggcgtc gacggcctcg aggtgtgccg
      241 gcggctgaag tccgacgcca agacccgcgg catccccatc gtcatgctga cggccaaggg
      301 cagcgaggcg gacgtggtgg ccggcctgga gctcggcgct tccgactacg tgaccaagcc
      361 gttcagcccg cgggtgctca cggcccgcat cagggccgtg ctccgccgcg gcgacgagac
      421 cgccgacgac gaagcgacgg tccacatcaa ggagctcacc atccatcccg gcaggcacaa
      481 ggtgctcgtg ggggagaagc cggtggagct caccgccacc gagttccgca tcctcctgta
      541 tctggccaag cgtcccggct gggtgttcac taggcagcag atcgtcgatg ccgcccaggg
      601 ggacgactca ttcgtcaacg accgcttcgt gaccgaccgc tcggtcgatg tgcacatcgt
      661 ctcgctgcgc cgcaagctcg gttgctgcgg cgcctacatc gagacggtcc gcggcgtggg
      721 gtatcgcctg caggaccagt gagccatgcg tagaccgcgg ctgttctggc gtgtcttcgc
      781 cacctacctc gtcatcgtcg tgctgtgcac cgccgcggtc ggcttctacg cggtgcgctc
      841 ggtgcgcgac ttctacgtgg accacaccga gcgcgagttg caggcgcggg cggcgctcgt
      901 gcgcgagcag atcctcccgg ccattctcgc cgacacgccg gaggagctcg aggcgctcgt
      961 gcgtcggctc ggcgattcgt cgggaacgcg catcacgctc atcgccgtgg accggccggg
     1021 gtatggcaga ggcgaagtcc tggccgactc ggactccgac ccggtggaga tggagagcca
     1081 cgccacccgt cccgaggtcc tcgcggccct ccagggacgg cctggccgcg ctatccggta
     1141 cagcaggacg ctgtggcagg acatgatgta cgtcgccatc cccgtcactg aagacggccg
     1201 tctcacgacc gtcgtgcgta cggccgtccc cctcacccgc gtgaacaacg cgctcgcctc
     1261 cctctacggc agcatcgccg tgagcgcgat cgtcgtcgcg gtgatcgccg cgatcatcgg
     1321 cctcaccgtg tcccggcgca tcagcggaca gatgcgcgcg atcaaggtcg gcgccgagcg
     1381 ccttgcggca ggggacttcg cgcacaagct gtccgtgccg cgcgtcgagg agttcgcgtc
     1441 cgtggccgag agcatcaacc ggatggctga acagctggac gacaagctgc gccggctcac
     1501 ccatgagcgc aacgagcgcg aggcggtgct cgccagcatg gtcgagggcg tgctcgccgt
     1561 cgacatcgac gagcgggtga tcgccgtgaa cgccgccgcc gcgcgcctcc tggacaccga
     1621 ccccgcgtcg gccgagggca aggcgatcca ggaggtagtc cggaaccccg acctccagca
     1681 cgtggtggcg cagacgctgg acggccaccg accggtcgag gccgacatcg tgatacgcgt
     1741 cggtgcggag gagcgcaacc tccaggccaa cggcacgttg ctgcacgggg acgacgacgg
     1801 agacgacgtg ggcgccgtcg tcgtgctcaa cgacgtgacg cgcctcaaga ggctcgaggc
     1861 ggtgcggcgc gacttcgtcg ccaacgtgtc ccacgagctc aagacgccgg tcacctccat
     1921 caagggcttc gccgagaccc tcgaggacgg cgcgctggac gatcctgcgg cggctcgccg
     1981 cttcgtgcgc atcatcgcgg ggcaggcgga ccgcctgaac tccatcatcg aggacctcct
     2041 ggcgctctcg accctggagc agtcgggtga cagcccgctc ctgcagctcg aggaagccga
     2101 cctctgcgac gtggtcgccg tcgcgctgga ggtctgcggc cccaaggcgg aggccaagag
     2161 catcgagctg cgcgaggact gccccgggcg tctcctggcg cgcgtgagcc cacccctgct
     2221 ggagcaggcc gtggtcaacc tcatcgacaa cgcggtgaag tacagcagcg aaggcagcac
     2281 cgtcgtcgtc acgctcgagg agaggggcga cgaggtcgtc gtgagcgtca ccgacgaggg
     2341 ccaaggcgtc gcgcgcgagc atctcccgcg gctcttcgag cgcttctacc gcgtcgacaa
     2401 ggcgcgcagt cgcgacctcg gcggtaccgg cctgggtctc gcgatcgtca aacacgtcgc
     2461 ccagatccac ggcggccgcg tctcggtcga cagcctgatc ggccgcggca gcaccttccg
     2521 cgtccacctg cctcgcggct gagcagaccg gactggggcg gcgcgccccc gctgcagccg
     2581 gatcgtcgag cgacgagcgt gggccttcgt gacctgccca ccatgaccaa gagtgcgctc
     2641 aacgtgcttg acgccaaccg caagggcttc ttcctgatga tcgagggtgg cgccgtcgac
     2701 tgggccggcc acgccaatca gcccggccgc gccatcgaag agcagatcga cttcaacaag
     2761 gccgtggagg cggtggtacg ctgggtgcag cgcaacagcg actgggacga gacgctcgtc
     2821 atcgtgaccg gcgaccacga gaccggctgt ctctggggtc ccggcagcgg cgtcccgggt
     2881 ggcaacggca cgtggacgcc actcgtcaac aacggtgagg acgtcacccc cggcatgcag
     2941 tccaacagcg gtgaccacac caactcgctg atcccgttct tcgccagggg cgagggcgca
     3001 gccgatctcc gcgccgccgc catgaaggcg gacccgatcc gcggcaagta cctcgacaac
     3061 acggacatcg ccaaggtgat cctcgccgac atgcggtaga ggacctgcac acccagcggc
     3121 tgaaacgggg cgggggcggc cgcagcggtc gctcccgccc cgtccgatga cacagaagga
     3181 gaagcatgac cgatcctcaa cgacgcacga agatcgtggc gacactgggc ccggcgtcgc
     3241 gcgatgagcc tgtgctggcg cgtctcctcg agagcggcat ggatgtggcg cgcatcaacc
     3301 tttcgcatgg gacaacggac gagcacgccg cgaccatcgc gcttctgcgc cgggtcgcgg
     3361 ccgaacgcgg cgtgtcgctc gcgatcctca tcgacctccc cggcccgaag ctgcgtacgg
     3421 gggacctgcc gcggccgctg accgtcaggg cgggggaagt cgtgaccctg gggccctcag
     3481 cggccgcgca gctgccggtc aacttcccgg agatgcttcc tcacctcacc gagggtgagc
     3541 tcatcctcgt ggacgacggc gccgtggccc ttcgggtcgc ggccgtctcg ccgacggtcg
     3601 tgaccctgga ggcgttgaac gacggcgcca tcgggtcgag gaagggcgtg aacctgcccg
     3661 atactcagct accgatcgct gcgttcaccg ggaccgacag cgatctgctg gcgtggggcc
     3721 tgcagcagga catcgactac gtggcgctct ccttcgtgcg cgacgccgcc gacatcctcg
     3781 acctcaagag gcgcatcgcc gcggcgggcg gtgaccagct cgtggtcgcc aagatcgaga
     3841 ggaaggaggc gctcggcgcg tacagggaga tcgtcgccgc cgccgatgcg gtcatggtcg
     3901 ctcgtggcga cctgggcgtg gaggtcgacg cggccaccgt gcctgtctgg cagaagcgca
     3961 tcatccatgc ggccgtcacg gccggcaggc cggtgatcat cgccacgcag atgctgcagt
     4021 cgatgatcgc atcgcctcgc gccacgcgcg ccgaggcgag tgacgtcgcg aacgcgatct
     4081 acgatgccgc gagcgccgtc atgctctccg gggagacggc catcggcggc tatccggtgc
     4141 gggcggtcga gacgatgcgc gagatcgcgc tcgtcgtcga agccgacatc gagcgcgagg
     4201 gacgcgcccc gaggccgtgg accgaggggc gcaccggcgt ctccgacgcg atcagccacg
     4261 gcgcctgcga ggtggccgaa cgcatcggcg ccgcggccat cgtcacggcc accttctcgg
     4321 gcgccaccgc ccgcgccgtg gctcgcagtc tgcccgacca gccgatcgtc gccatcagcc
     4381 cgaaccagcg ggtggtgaac cagctggcgt tgtgctgggg cgtgcacgcc ctgctcggtg
     4441 ggcaccgggc cagtttcgag gagatcgtgc gcgaagccaa cgacctcgtc ctcgccggag
     4501 gctttggcct gcctggtgac tcggtggtca taaccgccgg gctgcagacc caccagtccg
     4561 gcaagaccaa cctcatcaag gcgcacatcc tcgcttgagc aaggtggggc caggccggca
     4621 cgggagaccg cctgccacgg ctcagatgcg ccgctcacgc gaccaagaca tcctcctcat
     4681 caatcgccaa tgaagagtct ccagacttgc ctccggaagg cccagctcga cgtggctgcc
     4741 cgatggggcc gggagttcgc tccctgacct cgcggcaacc acatcgtccg ctaacagtcc
     4801 gctaacactg gctgggaaac atgggccgtg ccctgcgggg caacggccag ggaggcgacg
     4861 cgagcacccg cgctcggcgc gagtctcgac aggccgccca aaggaccgct accacggaga
     4921 ggcacaggag gagtaatgag gcgtatacgt tggttcgcga tggtcgcagc tgtgatggcc
     4981 atcgcgctgg tgggagtggc gtgcgggggc gatgagcctt ccgacggcgc cagcggtgac
     5041 ggaacggcag ccctctcggg tgacatccgt atcgacggct cgagcacggt gggtccgctc
     5101 accgaggccg ccgcggagtt cttccaggag gagaacccgg acgtgcgcgt caccgtcggc
     5161 atctccggca cgggcggcgg cttcgagaag ttctgcgccg gggagaccga cgccaacgac
     5221 gcctcgcgcc agatcgaagc ggacgaggaa gagctttgcg ccacagcggg agtcgagtgg
     5281 gtcgagatcc aggtcgcgaa cgacggtctc gccgtggtcg tcaacccgga gaacgactgg
     5341 gcggacagcc tcacggtcga gcagttgaag atgatctggg acgagggcag cacgatcgac
     5401 aactggaacc aggtcgatcc gagcttcccc gacgttcctc tggagctctt cggcgccggc
     5461 acggattcag gcacgttcga ctacttcacc gaggcggtca acggcgagga gggcgtcagc
     5521 cggaccgact accaggccac cgaagacgac aacgtctcgg tccagggcgt ggccggctca
     5581 aagggcggca tggcctactt cggcctctcc tactacgagc agaacgccga ccagttgaag
     5641 ctcgtggcga tcgacaacgg caacggcccc gtgaccccgt ctcttgagac cgtgcagtcg
     5701 ggcgagtaca cgccgctcag caggccgctc ttcatctacc cgtcggtcga gtcgcttcag
     5761 cgcccggaga tggcggcgtt catgcagttc tacctggaca acgcgcagag catcgccgag
     5821 caggccctgt tcgtgccgat cacggcggag cagaccgcgg ccgctcaggc agtgctcgac
     5881 gggatcgtcg gcggggcttc gccggccgcc gagtaaggaa gacatggcgg ctgtagccac
     5941 agaacggccc gcctccaggc ccctcgaggg cctggaggcg ggcagccgcc gttaccgcga
     6001 gaaggtcatc gtcggcagtc tgttcctgtg cgcggcgctc tcggtcctcg tgaccgtggg
     6061 gatcgtgctc gcgctggtga tcccgagcgt cgagttcttc ggcacggtca gcccggtcga
     6121 gttcttcacg gggacggagt gggcgccgct cttcaagaac tcgaagttcg gcgtcctgcc
     6181 gctgctctcg ggtacgctgg tcgtcaccgt catcgccgcg ttgatctgcc tgccgctcgg
     6241 cctgctggcg gcgatctacc tgagcgagta cgcggcgcgg aggacgagga gcttcgtcaa
     6301 gcccatcctt gagatcctcg ccggtatccc caccgtggtc tacgggttct tcgccctgac
     6361 gttcgtcacg ccgctgcttc gggagaactg gctgttcgag acgccacccc aggtgttcaa
     6421 cgcgctctca gcgggtctcg tgatgggcat catgatcctc ccgaccgtcg cctccatctc
     6481 cgaggacgcc atgtacgccg tgccgcaatc cctgcgtgcc ggtgcggccg ccctcggcgc
     6541 cacgaagtat gagatctcca cacgcgtcgt cgtgccggcg ggcctctcgg gcatcatcgc
     6601 tgcgttcgtc ctcggcatca gccgggcggt cggcgagacg atgatcgtgc tgatcgccgc
     6661 aggcggcacc cccaatctca cgctcaatcc ggtggaagcc atgcagacca tgacggcgtt
     6721 catcggcgct gcggggctcg gcgaccagtc gaccggcagc atcggctaca agaccatctt
     6781 cgcggtcggc atgacgctct tcgtcatgac cctgatcatg aacatcatca gcatccgcct
     6841 ggtgaggaag tatcgcgagg tctacgattg agcacgtcga ccccccagaa gagcgcatca
     6901 tcctccgccc ccatctcgct cagcagccgg cgtgggtggc ggcatcgcgc gcgggacggg
     6961 gccttccggg gcgccgtgac ggggagcgtc gtcgtcgggc tcgtcgtcct cgtcgcgctg
     7021 atcctgcaga gcctcatcaa gggctggccc agactcaatc tggacctgat cacgagcttc
     7081 ccctcgagct tccccgaccg cgccggcatc cagtcggcga tcttcgggac cttctggatg
     7141 gtgggcatcg tgctcgtcgt cgccatcccc atcggggtcg gagcggccgt ctacatggag
     7201 gagttcgcgg attccgaggc gtggtacaac cggttcatcg acctcatcat ccagaatctg
     7261 gccgctgttc cctccatcgt gtacggcatc ctggggctgg cgtttctgtc tcggggcctc
     7321 ctgaacctgg gtccggtggt gatcaccgcg ggcctgacgc tgtcgttcct ggtcctgccg
     7381 gtggtcatca tcgcgtcgcg cgaggcgata cgtgccgtac cgagctccat cctggacggc
     7441 gcgctcgctc tcggggccac acgctggcag ggggtgagca ggcaggtcct gccggcctcc
     7501 atcccgggca tcgccacggg atcgatcctc gcggtgtcgc gcgcgatcgg tgaggcggcc
     7561 cccctgctgc tgctcggggc tctgcagttc atcaccttca accccaccgg gctcgacagc
     7621 tcgttcaccg cgctgcccat ccagatcttc aactggatct caagacctca ggaggggttc
     7681 cacatcgccg ccgcggccgc catcatcgtc ctcctgtgca tcaccctgct gatgaacgcc
     7741 gtggccatct ggctccgcaa ccgcttccag cagacgtggt gaggcggaga tgaacgaggg
     7801 gtccatgagc gagaacagag gtgcagtcgt gatggatgag tcggagcaga gacccgagtc
     7861 gctgcctgaa gtcggcgtcc tccccgagac ggaggcggtc gacgtcctcc aggagagcgc
     7921 gaagctggcc gagatcaccc gggagcggcg ccgccaggcg aacgacggcg ccgacgctga
     7981 gaccgtgttc gagatgcggg acgtgtcggt gacctacgcc ggcagctacg ccgtccaggg
     8041 cgtcgacatg tccatccgca agaacgagat cacggcgttg atcggcccct caggatgcgg
     8101 taagacgacc gtcctgcgct gcctcaaccg cacgaacgac ctgatcccgc gcgccaaggt
     8161 cgagggcaag gtgctctacc acgggatcga cctctacggc cccaaggtcg aaccggtgga
     8221 ggtccgcagg cggatcggca tggtcttcca gaagccgaac ccgttcccca agtcgatcta
     8281 cgacaacgtc gccttcggcc ctcgggtcgg cgggttcaag ggcgacatgg acgagctggt
     8341 ggaacggtcg ctgcggcgcg cggcgctctg ggacgaggtg aagcgtgacc tcaaagcctc
     8401 cgccatggcg ctctcgggcg gccagcagca gcgcctctgc atcgcccgct gcatagcggt
     8461 cgagcccgac gtcatcctga tggacgagcc ctgctccgca ctcgatccgg tctcgacgct
     8521 caggatcgag gagctcatgt atgaactggt gtccgagtac acgatcgtca tcgtgaccca
     8581 caacatgcag caggcagccc gcgtcagcga ccgcaccggc ttcttcaccg tggctgagag
     8641 ccaggacgag aaggaccacc gcatcggcat gctcgtcgag ttcgacctga cgcccacggt
     8701 gttcaccaac ccgagcgatt cccggactga ggcctacgtg accgggcggt tcggctgagg
     8761 cgagccttcg ttccgaggcg cggacggcac gccggccagg ccgccgcctc ggcgctgctc
     8821 tcgcctgcac gccgtgcggg cgcagccggt ctcacgccgt cagtgcttca cacggtccag
     8881 ggacgccctg cgtgtcgtgg acgaccccgc gccggaagac gatgcggtgg ccacaggtcc
     8941 cggtggtctc ttgcgtgaag cccatggcca gatggaaggc gaccgactgg gtggagggcg
     9001 gggagacagc gcgcacgtag cgtcgcccgt tcatctgggc ggcaacgaag aagcgttcac
     9061 agagccgccg cccgaaacca aggcggcgga actccgggtg gacgccgatc atgtgcacgc
     9121 atgcctcgtc cgggtgtgcc tgggagagga atccgaggag gaagccgaca agcaccccgt
     9181 cactcgcctc gaacgcgaag ctggtcgacg tgaggtgaga gaagaagaca tgcggcaggt
     9241 acgagcccgc ggccgggccg ccccaccact cgtcgatgac ggcgaccacg cggtcgcagt
     9301 ccgacgttcg cacgtggcgc aactgcatgg tcaagacgcc cgccttgccg gccggggctc
     9361 cgtccgctcc aggaaccatc cgggacggat ggagaagagc atgcggcagc gctccgaaca
     9421 gaagaggtag gtgtcgccct tcacctgggt gctcagcagc gcctgttcct cggtcagctc
     9481 acggccgcat accgggtcgc atggaagccg cttgggcctt tcgctcattg aaggcacctc
     9541 cattcgcgct catctggctg gagcgccatc cgcggtgccc gtgctgcatt ctggtgcgcc
     9601 catgttcagg cgttgcgtgc ttcgcgtgag gcacgtgcat gcgcgcacgc tcgaacgggc
     9661 gccggcctgc gccggcgctc gacgggcatc ctgacgtcag gtgggggcaa cgccggccac
     9721 acgaggatcc cgaggtctgt tctgccacga cccggcacgg ccgttctcgc gaggcagaag
     9781 gcgctccggc cctgccgagt cggctcgaag gaacgtgcga tcagtccggc gttcgtccct
     9841 cggcgctgaa gtgccggaca tcgacgagct cgcgcaactc ccccgtgacg aggaacacgg
     9901 tctgctcggc cacatccacc gtgttgtcgc cgatgcgctc gagtgcgcgc gccatgagca
     9961 ccatgaacat cgcccactcc cggagctcca cgtcgtcccc gatgtcgacg gcgcgacgga
    10021 agacctggcg attgagctgg tcgacttcgg tatcctggag ggcgagatcc tcggcgagct
    10081 tgacgttgcg cgagacgaac gtctccttgg cctgcatgat ctgccgcccc acgagccgtc
    10141 ccatgtcttc gatggccgcg agcatctgct catcacgcgg cgactcgtgg ccggagagcg
    10201 gcaccagctt ggcgatgttc acacactggt cgcccatccg ctccaggcac cagacgacat
    10261 gcagcagggc ggcgaccagg cgaaggtcgc cggccactgg ggcctggctc gccagcagcg
    10321 tgatcgcccc atggtggatg tcgaggtaac ggtggttgat cgggcggtcg gcggcaacga
    10381 cggtcgcggc gaggcgagcg tcacctctcc tcacggagcg tatggcgctc acaagctgct
    10441 cgccgacctc atcgaagccg gccagagccc gccgctcgag ctcctcgagt tgcgttatga
    10501 acgccgtgcg tgtctcgcgc tccatgggct aggccttacc ctttccagcc gagtgccacg
    10561 ccagagcgcc ggcgccggtt cgcggcgcga ggaggaggta gctgctcccg cacgaggggc
    10621 agtcggcagt tccgtagcgc cctgccccgc gagggaacgc ggcgaggaac tcctcgccgc
    10681 acgcctcaca ggtgaggtgt gcggtcagtg ccgagtgcgc cggggcggcc gcgggcgggc
    10741 cggcgtcagt cttcttcgct gacaccggcc cgcccggctc cggcttcggg cggcctcggc
    10801 gcaccgtcat cgcccgcggc gcggggggac gctgcggagc cggcaggctc gtcacggacg
    10861 tggccgtgcg tgccgttcgc ctcggcctca cgaagctcct gccggcgctc ttcctccgac
    10921 agcgcggagg cacggacgct gcggagctga cgccggatct tcatgcgccg ggtgatcatg
    10981 gtcacagcct gccggcggcg tgttaaggag gcgtgagcat ggcatctcga cgccgtgaat
    11041 ccggcgagcg cagaatgctc cgtccttaca ggcttcaacg cgaggctcgt gtctctctga
    11101 cgccgcgcta accgtccgct aacacccggg aggtagcttc catcggcatc ggcgcgcacg
    11161 cagggcgagc cggctggcga cgacagctcg tgggtcgaga gacccgggct gaggtagggg
    11221 acgatgagac atctgcaacg tgagatagac cgcctcaagc ggaaggtcct ggctctcggc
    11281 gcgctggtgg aggagaacct ccgcctcgcc ttccaggcca tcgaccagcg cgacgcggcg
    11341 aaggcgcggc gcgtgatcgc gaccgacgtg ttgatcgacc agaacgaggt cgatgtcgag
    11401 gaggagtgcc tcaagatcct cgcgctctac cagcccgtgg ccggcgacct gcgcttcgtc
    11461 gccgcggtca tcaagatcaa cagcgagctg gagcgcatcg gcgacctctc cgccaacatc
    11521 gccgagcgcg cactccagct ggtcgacgag cacccggtgg ccgtgcctca caacgtcgcg
    11581 gtgatggcag atcgtacgtg gaccatcctc gagaagtcgc tcgacgccct cgttcgccag
    11641 gatgcggtga tggcgcggga ggtcctcgtc gccgacgacg aggtcgacgc gctctatgcc
    11701 gagctcatcg aggagctcaa ggcggtgatc cgcgccgacc tcgagcatct cgacgcgatc
    11761 gtgctgctgt tcagcgtcgc gcgctacctc gagcggctgg cggaccacgc caccaacatc
    11821 gccgaggacg tcctctacat ggtcgaaggc gagatccagc ggcacaacca gtcggcagtg
//
LOCUS       DASUMX010000340         2270 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_280664, whole genome shotgun sequence.
ACCESSION   DASUMX010000340 DASUMX010000000
VERSION     DASUMX010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280664"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..1176)
                     /locus_tag="VFZ86_15900"
     CDS             complement(79..1176)
                     /locus_tag="VFZ86_15900"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX5643795.1"
                     /translation="MNRRSIAAACTLSAVLALAAGCGDRDAAESKAMVRKTPVTTAEA
                     VRQRVDAVETTVGRIEATASPALAAETSGRVVRLHVDGGSVVRKGQVLAELDGESQRL
                     SVASARAGVERLEALLANQQRTVKRYQELRSKAVSESMLEEAIAQESARQAELNDARA
                     RLAEAEYRLDRTRIKSPVDAIVERRLISVGDYVSPGTPIVTIVGRDGIRAVLPFPERL
                     SGQLRPGLAVTLEQPARPGEPVTGTVTEVRPMVGTNNRAVEALVDLPGASDWPAGGSV
                     TAQLVLATREGVVVPASSVVRRPAGDVVYVLTGEKVAERKVTVGLRDVERAEVLTGVE
                     PGETVILGGAGFLTDGALVAVREPGKADATP"
     gene            complement(1173..1835)
                     /locus_tag="VFZ86_15905"
     CDS             complement(1173..1835)
                     /locus_tag="VFZ86_15905"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5643796.1"
                     /translation="MNRAASRIRAPRAPSPGARRIEAAARRLFANRGYAGTSMAEIAA
                     AARVSKATVFHHFRSKRTLYESLVGDAMAGFREQLVPLLDAEGDLQGSLREFASAHVE
                     RLTRMQGTMRLIAREMLSGTSASSDLLAGSGMSQNFSLLVDALRRGQAQGTVRAEVDP
                     GLAVFLLLSANWFLFQTGTLTRRNPDLAVTISTETYAAELARLLYFGLAPTAGPGHQE
                     SP"
     gene            1954..>2270
                     /locus_tag="VFZ86_15910"
     CDS             1954..>2270
                     /locus_tag="VFZ86_15910"
                     /inference="COORDINATES: protein motif:HMM:NF037928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host attachment protein"
                     /protein_id="HEX5643797.1"
                     /translation="MSRIRIVVADQAEAIFYDAPSLDARPKEVGRISDPAAHLHDRDL
                     ASDRPGRSYESVGHARHAIERENDPRWQEAVRFARRISCRLDDALQKGEFEELIVVAG
                     PPFL"
BASE COUNT          317 a          813 c          832 g          308 t
ORIGIN      
        1 ccacgagccc gaacagcacg aacacggcgc tcgccatgaa ggcgagcacg tgccggcgga
       61 ccgagagttc cgcgagcttc atggcgtggc atccgccttg cccggctcgc gcacggcgac
      121 gagcgcgccg tcggtgagga acccggcgcc gccgaggatc acggtctcgc ccggctcgac
      181 gccggtcagc acctcggcgc gctcgacgtc gcgcaggccc accgtgacct tgcgctcggc
      241 caccttctcg ccggtgagca cgtacacgac gtcgccggcc gggcggcgca ccacgctcga
      301 ggccggcacc accacgcctt cgcgcgtggc gagcaccagc tgcgccgtga cgctgccgcc
      361 ggcgggccag tccgaggcgc ccggcaggtc caccagcgcc tcgaccgcgc ggttgttggt
      421 gcccaccatc gggcgcacct cggtgaccgt accggtcacg ggctcgcccg gccgggccgg
      481 ctgctcgagc gtcacggcca ggcccggccg gagctggccg gacagccgct cggggaaggg
      541 cagcacggca cggatgccgt cccggcccac gatcgtgacg atgggcgtgc cgggcgacac
      601 gtagtcgccg accgagatga gacgccgctc gacgatcgcg tccacggggc tcttgatccg
      661 cgtgcggtcg aggcggtact cggcctcggc gaggcgggcc cgtgcgtcgt tgagctccgc
      721 ctggcgggcg ctctcctggg cgatcgcctc ctcgagcatg ctctcggaga cggccttgga
      781 gcgcagttcc tggtagcgct tcacggtgcg ctgctggttc gcgagcagcg cctcgaggcg
      841 ctccaccccg gcgcgcgccg aggcgaccga aagccgttgc gactcgccgt cgagctccgc
      901 cagcacctgc cccttgcgga cgacgctgcc gccgtccacg tgcaggcgca cgacgcgccc
      961 cgaggtctcg gcggcgagtg ccggcgacgc cgtggcctcg atgcggccga cggtggtctc
     1021 cacggcgtcc acccgctggc gcacggcctc ggcggtcgtg accggcgtct tgcggaccat
     1081 ggccttcgat tccgcggcgt cgcggtcgcc gcagcccgcg gcgagggcga gtacggcgga
     1141 caaggtgcag gcagcggcga tggatcgacg gttcatggcg attcctggtg gccgggcccg
     1201 gccgtcggcg cgagcccgaa gtacaagagc cgcgcgagct cggcggcata cgtttcggtc
     1261 gagatggtga cggcgaggtc cgggttacga cgcgtgagcg tcccggtctg gaacaggaac
     1321 cagttggcac tcaacagcag gaatacggcg aggccgggat cgacctcggc acgcaccgtg
     1381 ccctgggcct ggccgcgacg cagcgcgtcc accagtaatg agaagttctg cgacatcccg
     1441 ctgccggcga gcaggtcgct cgacgcggac gtaccgctca gcatctcgcg ggcaatgagc
     1501 cgcatcgtgc cctgcatgcg ggtgaggcgt tcgacgtgtg cggaggcgaa ctcgcgcagg
     1561 ctgccctgca ggtcgccttc ggcgtcgagc agcgggacga gctgctcgcg gaagcccgcc
     1621 atggcgtccc cgaccagtga ctcgtagagc gtgcgcttgc tgcggaagtg gtggaaaacc
     1681 gtggccttgc tgacgcgcgc ggctgcggcg atctcggcca tcgaggtacc ggcgtagccc
     1741 cggttggcga acagtcgccg cgcggccgcc tcgatgcggc gcgcgcccgg ggaaggcgcc
     1801 cggggggcac ggatgcggct tgcagcacgg ttcatcgcgg catgctagca ctgaccgatc
     1861 ggtcggtcag tcgtggcctg ccgctttcac ggattgcggc gcccgcggcg ccggcgcagc
     1921 ctgtaaccag gccccaacca gggggaggtc accatgagcc ggatccggat cgtcgtcgcc
     1981 gaccaggcgg aagctatttt ctacgatgcg ccctcgctgg acgcacggcc gaaggaggtc
     2041 gggcggatct ccgatccggc cgcacacctg cacgatcgcg acctcgcgag cgaccgcccg
     2101 ggccgcagct acgagagcgt cggccatgcg cgccacgcga tcgagcgcga gaacgacccg
     2161 cgctggcagg aggcggtgcg cttcgcccgg cgcatctcgt gccgcctcga cgacgccctg
     2221 cagaagggtg agttcgagga gctgatcgtg gtcgccgggc cgccgttcct
//
LOCUS       DASUMX010000341         6586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_856364, whole genome shotgun sequence.
ACCESSION   DASUMX010000341 DASUMX010000000
VERSION     DASUMX010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 6586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6586
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_856364"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="VFZ86_15915"
     CDS             complement(<1..239)
                     /locus_tag="VFZ86_15915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643798.1"
                     /translation="MAIVTYSNPGFGFGATYDDALLTHTDDDADPRLRATWAARIRTA
                     VAAAVVFVPWTATADDIARGVAPSLLLTTDARRLGP"
     gene            338..2116
                     /locus_tag="VFZ86_15920"
     CDS             338..2116
                     /locus_tag="VFZ86_15920"
                     /inference="COORDINATES: protein motif:HMM:NF021375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="HEX5643799.1"
                     /translation="MRLIPPHIDPDTPSDGEKWVFAQFATAGRPVAGASDAGGGSGLD
                     TSGWTVLHSQDLAHHRRQMEGEIDFLVVAPGLGVLVIEVKGCHKLRRERGLWHYGADR
                     AGDPRGPFKQASEAMHSLRERLGRQRAHLHGVLFQSVVCFPFIDFSGVSEEWHPWQVI
                     DHAKLRQRPIAEWVAAALRQARERTEELRKSWFDPHAGEPTADQCGEIVKVLRHDFEF
                     YESPKARAQRIDEEIRHYTESQFEALDHMRRMPRVVFDGPAGTGKTLLAIEAARRARS
                     EGRRALLLCFNRPLAEWLREQTAGLCDATTVSDHMVRAAGIPAGSPRFEEHGFWDRLP
                     LQAGEALIEKPPDYDELILDEAQDVLRHQFLDVLDFSIAGGLKEGFWRVFGDFRHQAI
                     YDDSVDLEAFCADDGRTCAVYALDENCRNAPAVAALACAGAGISPGGAGADGYARCLR
                     PDDGDPPEVRFYRDAAQQRDLLCAALQDLRDAGITGPQVAVLSTRSDAACAASGVTEG
                     PWKDRLAPLVRDTPRGPVTDFKSGTTHFASIHRFKGLEAHAVVLTDIERLDSARERDL
                     FYVGATRATQRLIVLAHESLRARLPG"
     gene            2131..2898
                     /locus_tag="VFZ86_15925"
     CDS             2131..2898
                     /locus_tag="VFZ86_15925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007514545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEX5643800.1"
                     /translation="MKIALFITCVADTLFPETGIAVVRVLERLGHEVVFPAGQTCCGQ
                     MHVNSGYRDDALRLARRFVDVLGRDDGVDAVVSPSSSCVGWVRELLPGVAREAGDERL
                     AADAEALGGRLFELSELLVRRLGVEDVGAAYPRRVTLHPTCHSQRVARIGDAPQRLLA
                     KVKGLELLELPEATECCGFGGTFSIKNAAVSLAMLEDKCRNILATGADVVTAVDPSCL
                     LHIGGGLSRLAPAAPGGPGPAVRAVHLAQILAAEAGA"
     gene            2895..4352
                     /locus_tag="VFZ86_15930"
     CDS             2895..4352
                     /locus_tag="VFZ86_15930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018383014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein B"
                     /protein_id="HEX5643801.1"
                     /translation="MSRAHDFPAAARAALADAQLRANLRGATHTIRARRAAAVAELPD
                     FEGLREQARRVKDDALDHLDELLARFEAAATAAGAQVHHAADAGAANAFVAAIARDHG
                     VTELVKTKSLATDEIGLDAALAATGVEAVETDLAELIVQLAGDTASHILVPAVHYSRG
                     QVRDLFLRTIARGRELSDDPRELAEVSRVYLRERFLRAGMGVTGANAGVAETGTVCVV
                     ENEGNGRMCTTLPSVLVTVMGIEKVVATLDDLALLLRLLPRSATGERMSSYVSLLTGI
                     TPGDGPREYHIVLLDNGRRDALADPVGRQALRCIRCSACLNVCPVYTRVGGHAYGSIY
                     PGPIGAILTPQLWGMERHAELPFASSLCGACAEVCPVGIDIPEVLLHLRARAVREQAP
                     AAEKVVYGAAAWAFGAPRRFAAAQKAVRAAERPLARRGLVRRLPGPLAGWTEGRDLRP
                     VARETFREWWARERGSEERPGRCREKPPVREEKAP"
     gene            4349..4894
                     /locus_tag="VFZ86_15935"
     CDS             4349..4894
                     /locus_tag="VFZ86_15935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014686679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HEX5643802.1"
                     /translation="MNSRRGEAVVAGFAERLALQHVEVRRVAADAVAAAVAGVCAELG
                     VRRLGVPPALPEPWRPAGVDVVEDHGLAPAELDTLDAALTGCTLAIAETGSLVLAAGP
                     DDGRRALSLVPDVYICVVREDQIEPDLPAAMPRIAGVVRDSGHPVVIVSGPSATSDIE
                     LHRVEGVHGPRRLVVLLVGPG"
     gene            5013..5177
                     /locus_tag="VFZ86_15940"
     CDS             5013..5177
                     /locus_tag="VFZ86_15940"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HEX5643803.1"
                     /translation="MATYAFVCTKCKTSFEVTCHMDEREAQAVCPKCGSHKVGQVYTA
                     AFSSPPPDKY"
     gene            5289..5804
                     /locus_tag="VFZ86_15945"
     CDS             5289..5804
                     /locus_tag="VFZ86_15945"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX5643804.1"
                     /translation="MAVQVGQPAPDFTLKGVVDGEFVDVKLSDYRGKWVVLFFYPLDF
                     TFVCPTEIRGFAGHNEEFEKLGAQVLGASVDSEHSHKAWIERDFGSLPFPLLSDIRRE
                     ATEAYGVLLPDGVALRGTFIVDPEGTLKYAVVHDNNIGRNTDETLRVLQALQTGELCP
                     LDWRPGQATLS"
     gene            5924..6226
                     /locus_tag="VFZ86_15950"
     CDS             5924..6226
                     /locus_tag="VFZ86_15950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643805.1"
                     /translation="MATIDEWFDQRTLMELVPIDVYVRGASAVEAGAVRVVQYDETRL
                     LTRVEAGDRVVDAEWSLDGEQPVFACSCGEATERPCEHLIASALSTWPGEAPPEDE"
     gene            6282..6509
                     /locus_tag="VFZ86_15955"
     CDS             6282..6509
                     /locus_tag="VFZ86_15955"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX5643806.1"
                     /translation="MPIYEFQCAKCGERFEIVSSHAEREEKAVCPSCGGRDVSQVFGG
                     FRVGISRTRLNPGVFERKKGQAPRYKPPAGE"
BASE COUNT          882 a         2377 c         2442 g          885 t
ORIGIN      
        1 ggtcccagcc gcctggcgtc tgtggtcagc agcaggctcg gggcgacgcc gcgggcgatg
       61 tcgtctgccg tcgcggtcca ggggacgaag acgacggctg cggcgacggc cgtccggatg
      121 cgcgctgccc aggtggcgcg cagccgcggg tcggcgtcgt cgtcggtgtg cgtgagcagg
      181 gcgtcgtcgt aggtggcgcc gaacccgaag ccggggttgc tgtacgtgac gatggccatg
      241 gcgtgatccg atcgtggtgg gtcctcagca tatgtgagac ggacagcggg tgttcccggt
      301 tcgaggagta ggcgaacgtc ccggcgaaca aggcgccatg cgcctgatcc ctccgcacat
      361 cgaccccgac acgcccagcg acggcgagaa gtgggtgttc gcccagttcg cgaccgccgg
      421 gaggccggtg gccggcgcgt ccgacgcggg aggcggctcc ggtctcgaca cctcgggctg
      481 gaccgtcctg cactcgcagg acctggccca ccaccgccgc cagatggagg gcgagatcga
      541 cttcctcgtg gtcgcgcccg ggctcggcgt cctcgtcatc gaggtgaagg gctgccacaa
      601 gctgcggcgc gagcgcgggc tgtggcacta cggtgcggac cgcgccggcg atccgcgtgg
      661 tccgttcaag caggcctccg aggccatgca cagcctgcgc gaacggctcg gccgtcagag
      721 agcgcacctg catggggtcc tcttccaatc cgtcgtctgc ttcccgttca tcgacttctc
      781 cggcgtgagc gaggagtggc acccctggca ggtcatcgac cacgcgaagc tgcggcagcg
      841 gcccatcgcg gagtgggtcg ccgccgccct gcggcaagcg cgcgaacgca ccgaggagct
      901 gcgcaagtcg tggttcgacc cgcacgcggg cgagcctacg gccgaccagt gcggcgagat
      961 cgtcaaggtg ctccgccacg acttcgagtt ctacgagagc cccaaggcgc gggcgcagcg
     1021 catcgacgag gagatccgcc actacaccga gagccagttc gaggcgctgg accacatgcg
     1081 gcgcatgccg cgcgtggtct tcgacggccc cgcgggcacg gggaagacgc tgctggcgat
     1141 cgaggcggcg cggcgcgccc gctccgaggg ccgccgcgcc ctcctgctct gtttcaaccg
     1201 cccgctcgcc gagtggctgc gcgagcagac ggccgggctg tgcgacgcga ccaccgtctc
     1261 cgaccacatg gtccgcgccg ccggcatccc ggccggctcg ccacggttcg aggagcacgg
     1321 cttctgggac cggctgcccc tgcaggcagg cgaggcgctg atcgagaagc ctcccgacta
     1381 cgacgagctc atcctggacg aggcgcagga tgtgctgcgg caccagttcc tggacgtgct
     1441 cgacttcagc atcgccggcg gtctcaagga gggcttctgg cgcgtcttcg gcgacttccg
     1501 ccaccaggcg atctacgacg actccgtgga cctcgaggcc ttctgcgcag acgacggacg
     1561 cacgtgcgcg gtctacgctc tggacgagaa ctgccgcaac gcgccggcgg tggcggcgct
     1621 cgcatgcgcc ggcgccggca tcagcccggg cggcgccggc gcggacggct acgcccgctg
     1681 cctgcggccc gacgacggcg acccgccgga ggtcaggttc taccgggacg ccgcgcagca
     1741 gcgtgatctg ctctgcgcgg cgttgcagga cctccgcgac gccggcatca ccggaccgca
     1801 ggtcgcggtg ctcagcacgc ggagcgacgc ggcctgcgcg gcgtccggcg tcaccgaggg
     1861 gccctggaag gatcgtctcg cgccgctcgt ccgcgacacg ccgcgcggcc cggtcaccga
     1921 cttcaagtcg ggcacgacgc acttcgcgtc gatccaccgc ttcaaggggc tcgaggcgca
     1981 cgccgtcgtg ctgaccgaca tcgagcgcct cgacagcgcg cgggagcgcg acctcttcta
     2041 cgtcggcgcc acgcgcgcca cacagcggct catcgtgctc gcccacgagt cgctgcgcgc
     2101 ccggctgccg ggctgaccgc ccgcgcgtgc atgaagatcg ccctgttcat cacctgcgtc
     2161 gccgacacgc tgttcccgga gacagggatc gccgtcgtgc gcgtgctcga gcggctgggc
     2221 cacgaggtcg tgttcccggc cgggcagacc tgctgcgggc agatgcacgt caacagcggc
     2281 taccgggacg acgcgctgcg cctggcgcgg cgcttcgtgg acgtgctcgg gcgggacgac
     2341 ggcgtggacg cggtggtctc gccgtcgtcc tcgtgcgtgg gctgggtgcg cgagctgctg
     2401 cccggcgtcg cgcgtgaggc cggtgacgag cgcctggccg ccgacgccga ggcgctcggc
     2461 ggcaggctct tcgagctctc cgagctcctc gtccgccgcc tcggcgtgga ggacgtcggc
     2521 gcggcgtacc cgcggcgcgt cacgctgcac cccacctgcc actcgcagcg cgtggcgcgc
     2581 atcggcgacg cgccgcagcg gctgctcgcc aaggtgaagg gcctcgagct gctcgagctg
     2641 ccggaggcca cggagtgctg cgggttcggc ggcaccttct ccatcaagaa cgccgccgtg
     2701 tccctggcga tgctcgagga caagtgccgg aacatcctgg cgaccggcgc cgacgtggtg
     2761 accgccgtcg acccgtcgtg cctgttgcac atcggcggcg ggctcagccg gctggcgccg
     2821 gcggcgcccg gagggccggg cccggcggtg cgcgccgtcc acctggcgca gatcctggcg
     2881 gcggaggcgg gcgcgtgagc cgcgcccacg acttcccggc cgcggcgcgc gcggcgctcg
     2941 ccgacgcgca gctgcgcgcc aacctgcgcg gcgcgacgca cacgatccgc gcgcgccgtg
     3001 ccgcggccgt cgcggagctg cccgacttcg aggggctgcg cgagcaggcg cggcgcgtca
     3061 aggacgacgc cctggatcac ctcgacgagc tgctcgcgcg gttcgaggcg gcggccacgg
     3121 cggcgggtgc gcaggtccat cacgcggccg acgcgggcgc ggccaatgcc ttcgtcgccg
     3181 cgatcgcccg cgaccacggc gtcaccgagc tggtcaagac gaaatccctg gccaccgacg
     3241 agatcggcct cgacgcggcg ctcgccgcga ccggcgtcga ggccgtcgag accgacctcg
     3301 ccgagcttat cgtccagctc gccggggaca ccgcctcgca catcctggtg ccggccgtcc
     3361 actacagtcg gggccaggtc cgcgacctct tcctgcgcac gatcgcgcgg ggcagggagc
     3421 tctccgacga cccgcgcgag ctcgccgagg tatcgcgcgt gtacctgcgt gagcgcttcc
     3481 tgcgtgcggg aatgggagtg accggcgcca acgccggcgt cgccgagacg ggcaccgtct
     3541 gcgtggtgga gaacgagggc aacggccgca tgtgcacgac gctgccgtcg gtgctcgtca
     3601 ccgtcatggg catcgagaag gtcgtcgcca cgctggacga cctcgcgctg ctcctgcgcc
     3661 tgctgccgcg ttcggcgacc ggggagcgga tgagctcgta cgtgtcgctc ctgaccggga
     3721 tcacgccggg tgacggcccg cgggagtacc acattgtgct gctcgacaac gggcgccggg
     3781 acgcgctggc cgacccggtg ggcaggcagg cgctgcgctg catccgctgc agcgcctgcc
     3841 tgaacgtctg ccccgtgtac acgcgcgtcg gcgggcacgc ctacggctcg atctaccccg
     3901 ggccgatcgg cgcgatcctc acgccgcagc tgtgggggat ggagcggcac gccgagctgc
     3961 cgttcgcctc gtcgctgtgc ggcgcctgcg cggaggtgtg ccccgtgggc atcgacatcc
     4021 cggaggtgct gctgcacctg cgggcgcggg cggtgcgcga gcaggcgccc gcggccgaga
     4081 aggtcgtgta cggcgccgcg gcgtgggcct tcggcgcgcc gcggcgcttc gccgccgcgc
     4141 agaaggccgt cagggccgcg gagcggccgc ttgcccgccg gggcctggtg cgccgtctcc
     4201 ccggcccgct cgccggctgg accgagggtc gcgacctccg gccggtcgcg cgcgagacgt
     4261 tccgcgagtg gtgggcgcga gagcgcggtt cagaagagcg gccggggcgc tgcagagaga
     4321 agccgcccgt gcgcgaggag aaggcgccgt gaacagccgc cgtggagagg ccgtcgtcgc
     4381 cggcttcgcc gagcggctgg cgctccagca cgtggaggtc cgccgcgtgg ccgcggatgc
     4441 cgtcgcggcg gcggtcgccg gcgtctgcgc cgaactcggc gtgcggcggc tcggcgtccc
     4501 gcccgccctg ccggagccct ggcgcccggc cggcgtcgac gtggtcgagg accacggcct
     4561 cgcgccggcc gagctcgaca ccctggacgc cgcgctcacc ggctgcacgc tcgcgatcgc
     4621 cgagacgggg tcgctggtgc tcgccgccgg ccctgacgat gggcggcgag ccctctcact
     4681 cgtccccgac gtctacatct gcgtcgtccg cgaggaccag atcgagccgg acctgccggc
     4741 ggcgatgcca cgcatcgccg gcgtcgtccg cgactccggc caccccgtgg tcatcgtctc
     4801 cggcccgtcc gcgacctccg acatcgaact ccaccgcgtg gagggggtac acgggccccg
     4861 gcggctcgtg gtgctgctgg tcgggcccgg ctgagcgcct ccgcccgcgg caccgggcgg
     4921 cgatcgccgt ctcgcgggtg aactgtccca acgccggacg agccgggaag acagagggtg
     4981 agaactgtcg ttccggaccg ggggtgaggg ccatggcgac ctacgcgttc gtctgcacga
     5041 agtgcaagac ctcgttcgag gtgacctgtc acatggacga gcgggaggct caggcggtct
     5101 gcccgaagtg cggcagccac aaggtcggac aggtgtacac cgccgctttc tcctcgccgc
     5161 cgccggacaa gtactgagcc tatgagcacc gtttcacgcc ggtatggccc gggtaccctc
     5221 gcatccagca gcgcaagacc agggtcggcg gcagagacgt ccccggccct tcacgacaga
     5281 ggagcgacat ggcagtacag gtaggtcagc ccgcgcccga cttcacgctc aagggcgtcg
     5341 tcgacggcga gttcgtggac gtcaagctca gcgactaccg cggcaagtgg gtggtcctgt
     5401 tcttctaccc cctcgacttc acgttcgtct gcccgaccga gatccggggc ttcgccggtc
     5461 acaacgagga gttcgagaag ctcggcgcgc aggtgctggg cgcgagcgtc gacagcgagc
     5521 acagccacaa ggcgtggatc gagcgggact tcggctcgct gccgttcccg ctgctcagcg
     5581 acatcaggcg cgaggccacc gaagcgtacg gcgtcctcct gcccgacggc gtcgcgctgc
     5641 gcggcacctt catcgtggac ccggagggca cgctcaagta cgccgtggtg cacgacaaca
     5701 acatcggccg caacaccgac gagacgctcc gcgtcctgca ggcgctccag accggcgagc
     5761 tctgcccgct ggactggcgg ccgggccagg cgacgttgag ctagaccgag atcgcgaaga
     5821 cggctgcggc gccgcgaggc ggccgcgccc ggcgcggggc gccgcgacgc cggagacggc
     5881 cgcgcggcgc ccctgccacg tccatcacgg gagagggaga gacatggcga ccatcgacga
     5941 gtggttcgac cagcgcacgc tgatggagct cgtgcccatc gacgtctacg tccgcggcgc
     6001 ctcggccgtg gaggcgggcg cggtgcgcgt cgtgcagtac gacgagaccc ggcttctcac
     6061 gcgggtcgag gccggcgacc gcgtcgtgga cgccgagtgg agcctggacg gcgagcagcc
     6121 ggtgttcgcc tgctcgtgcg gcgaggcgac ggaacgcccg tgcgagcacc tgatcgccag
     6181 cgcgctctcg acctggccgg gcgaggcgcc tcccgaggac gagtgacggc ggcgggcggc
     6241 gggcccccgc cgcgacgcgc gttctgccgt acaatcgctg catgcccatc tacgagttcc
     6301 agtgcgcgaa gtgcggcgag cgcttcgaga tcgtctccag ccacgccgag cgcgaggaga
     6361 aggccgtgtg cccctcgtgc ggcggtcgcg acgtctccca ggtcttcggc gggttccggg
     6421 tcgggatctc gcggacgaga ctcaaccccg gcgtgttcga gcgcaagaag ggtcaggcgc
     6481 cgcggtacaa gccgccggcc ggcgagtgag gcgggcggtc gcgtagcggc cgccgacggc
     6541 cgcattcctc gcggcgcatg ctgccccgta cggcgacgcc gcggtg
//
LOCUS       DASUMX010000342         6972 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_2150076, whole genome shotgun sequence.
ACCESSION   DASUMX010000342 DASUMX010000000
VERSION     DASUMX010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 6972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6972
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2150076"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..909
                     /locus_tag="VFZ86_15960"
     CDS             <1..909
                     /locus_tag="VFZ86_15960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014181065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5643807.1"
                     /translation="DGVSLAAEPGSVFGLLGPNGAGKSTAVKILTTLSRPDAGEAFVA
                     GHDVLRHPARVRLAIGCVAQKSGLDLQATGRENLVLQGRVYGMRGRELRVRAQELIER
                     LGLADAADRTVKTYSGGMQRKLDVAVGLMHRPQVLFLDEPTTGLDPEARVDMWSEIAR
                     LAGDEGLTILMTTHYLEEADGLARRLAIVDRGKVVAEGTPDELKGELRGDAIVVELEE
                     QPLDGEVRRALDKVPDLYDVAVEARALRARADNGARAVPTVLAALEAEGVKVASVTVA
                     RPSLDDVYLRYAGRAFSEADEEAKKP"
     gene            906..1682
                     /locus_tag="VFZ86_15965"
     CDS             906..1682
                     /locus_tag="VFZ86_15965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010988112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5643808.1"
                     /translation="MTHVISDTLFMTQRHFRNLLRQPAWILISLVQPVVWLLLYGALF
                     RRIVEIPGFGAGSYIDFLTPGIVVMTCLFSAGWSGMDLIEDIDRGVVDRFLVSPASRS
                     SLIAGRLVQGAIVGVIQSAIIVVLGFIVGAEYPGGIAGIAALTLGAVLLGTGVGALSN
                     AVGVLSRRVETMIAVSNFVLLPLTFLSSVFMARALMPGWMQSVATYNPVEWAVGAGRE
                     ALAASPDWSVVLARLGWLALFTFVCGWLATAAFRVYQKSI"
     gene            complement(1692..3275)
                     /locus_tag="VFZ86_15970"
     CDS             complement(1692..3275)
                     /locus_tag="VFZ86_15970"
                     /inference="COORDINATES: protein motif:HMM:NF012956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEX5643809.1"
                     /translation="MPTRLAGTAAPAVAALCALALAIGALLIAAAPAAAVPDDFSGRV
                     RATLKRADARLAAMDRRAGPRRFAYYTRGDGWHYSGPSGWAAGFHPGSLWSMYQWTGA
                     GRWRDRALQRQSWLGAEDIGPESQNLGGLFFPSYVRGYKLTGRDGLRRRSLQVSRSMA
                     ARFDPLVGAMRSRDGEEFNVIIDSLTKPRLLWWAVKAGGPARYDAIARSHALTIARDH
                     VRPDGSTVHAAFYDPATGALKRVDKGAAYADGSTWARGQAWAVLGFPAAYRETRDPRF
                     LEVARLVSDWWLRHVPDDMVPYWDFGAPGIPAAPRDTSAAAIAASGLIDLALLDPDEE
                     RRAAYAGAARETLASLMSPAYSSATWTPSVLLHGTYSWWMDITDRGLAYGDAFFLEAL
                     LRLRRLSPEKPPLPVVTARSATGVPSAAIDGSLSTSWAARGPQSIDLRLQGVREVGAV
                     RVALARGDDRAAPLRILVSGDGRHWRPVKRSLTSGEWAGFETLDFAPRTARWVRVSCD
                     GTTRGSLCRIAEIEVYPAL"
     gene            3473..4789
                     /gene="purD"
                     /locus_tag="VFZ86_15975"
     CDS             3473..4789
                     /gene="purD"
                     /locus_tag="VFZ86_15975"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEX5643810.1"
                     /translation="MRVLVVGGGGREHALVHSFARSPLADRIFCAPGNAGTAAEADNI
                     PVAADDLPALLDFASREEVDLTVIGPEAPLVLGLTDLFEEHGLAVFGPNREAAQLEGS
                     KAFAKEIMSDGKVPTGAYRRHERLEPALLDVEGRDDYPLVVKADGLAAGKGVLICKDV
                     DEAREAVHACFVAREFGAAGDVLITEEYLTGSEVSLLVLCDGSTAVPLAPAQDYKRIF
                     AGDQGPNTGGMGSYCPVPGFEQKAIDAAMEHVVEPVVAALRRRGVTYRGVLYAGLIVT
                     DAGIKVLEFNCRFGDPETQAVLPRLSSDLLEHCAAAANGDLESTEVLWTPQDCVTVVL
                     ASAGYPASSHKGDVIGGLAEAAALEGVTVYHAGTALRDGEVVTAGGRVLAVSALGDGF
                     SAARARAYEGVARIGFEGMQVRPDIAERAVKARAGELDLFPAGFAG"
     gene            4840..5289
                     /gene="purE"
                     /locus_tag="VFZ86_15980"
     CDS             4840..5289
                     /gene="purE"
                     /locus_tag="VFZ86_15980"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013709604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEX5643811.1"
                     /translation="MEETQTEVETILQGIQMEPVLVGIVMGSESDREVMDKAAAELDE
                     RKIRYEISVLSAHRDPEKVAHYAKTAQSRGLKVLIGGAGKAAALPGVLAAYTNLPVIG
                     VPIKTSDLGGLDSLLSIVQMPPGVPVACVAINGARNAAILAAKILDL"
     gene            5365..5745
                     /locus_tag="VFZ86_15985"
     CDS             5365..5745
                     /locus_tag="VFZ86_15985"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX5643812.1"
                     /translation="MGRHAIRSAVAPPPLGAASSGISSGHFVFVSAQLPLDAAGEIAG
                     ATAAEQADRALQNLAHQLRSTGLSLEDVVSLEIHLVDQADRASVDAALEARFLPPYPA
                     VTLSGVPWLPGGARLQVAAIAQRY"
     gene            5901..6281
                     /locus_tag="VFZ86_15990"
     CDS             5901..6281
                     /locus_tag="VFZ86_15990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007130436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX5643813.1"
                     /translation="MEKQVFASPTGAPAVGPYSPAVGAGDFVFVSGQIPLDPDGRIVG
                     YTPKDQARKALENLRATLQAAGLGLEHVVKTTIFLRDMDEFAAVNEVYGEFFQEPYPA
                     RSTVEVARLPKELHVEIEAVAYRG"
     gene            6274..6942
                     /locus_tag="VFZ86_15995"
     CDS             6274..6942
                     /locus_tag="VFZ86_15995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643814.1"
                     /translation="MGDAHRRTPRRAARALAVLLTALAALGAGALAGCGSPAENDPFV
                     GYWVGGGMQGQMTLVQIGKDEDGKYVVLANPDQPAGEAVREGDSLVVDTHAVVMRFTP
                     APDDRLSLEFSGDMFEEPAATTLKRTDATQYSDAAVGYGLAQIERGLAMWKGGGGKKY
                     PPPKEVTPSGLLGQMIRWPNNPFTGQPMQPGQSEGDYVYKQVAGGKKYSLKGYLSDGS
                     TIGE"
BASE COUNT          993 a         2554 c         2486 g          939 t
ORIGIN      
        1 gacggcgtga gcctggccgc cgagccgggc tccgtgttcg gcctgctggg gccgaacggc
       61 gccggcaagt cgacggccgt caagatcctg accaccctgt cccggccgga cgccggtgag
      121 gcgttcgtgg ccggtcacga cgtgctccgg cacccggcca gggtgcgcct ggcgatcggc
      181 tgcgtcgccc agaagtccgg gctcgacctg caggcgaccg gacgcgagaa cctggtcctg
      241 cagggccggg tctacgggat gcgcggcagg gagcttcgtg tgcgcgcgca ggagctcatc
      301 gagcggctcg ggctcgcgga cgcggccgac cgcaccgtca agacctactc cggcggcatg
      361 cagcgcaagc tcgacgtcgc cgtcgggctc atgcaccggc cccaggtcct cttcctggac
      421 gagccgacga cggggctcga ccccgaggcg cgcgtggaca tgtggtccga gatcgcgcgc
      481 ctcgccggcg acgagggact gacgatcctg atgacgacac actacctcga ggaggccgac
      541 ggcctggcgc ggcgcctcgc catcgtcgac cgcggcaagg tggtcgccga gggcacccca
      601 gacgagctca agggcgagct gcgcggcgat gccatcgtgg tcgagctcga ggaacagcca
      661 ctcgacggcg aggtgcgccg ggcgctggac aaggtgcccg acctgtacga cgtcgccgtg
      721 gaggcccgcg cgctgcgcgc gcgggccgac aacggcgcgc gcgccgtccc gaccgtcctc
      781 gccgccctcg aggccgaggg cgtcaaggtg gcctcggtga ccgtggcccg gccctcgctc
      841 gacgatgtgt acctgcgcta cgccggccgt gcgttcagcg aggccgacga ggaggcgaag
      901 aagccatgac gcacgtgatc agcgacaccc tcttcatgac ccagcggcac ttccgcaacc
      961 tcctccggca gcccgcctgg atcctcatct cgctggtgca gcccgtcgtg tggctgctgc
     1021 tgtacggcgc cctgttccgg cggatcgtgg agatccccgg gttcggcgcc ggcagctaca
     1081 tcgacttcct caccccgggc atcgtcgtca tgacctgcct gttcagcgcc ggctggagcg
     1141 gcatggacct gatcgaggac atcgaccgcg gtgtggtgga ccggttcctg gtcagcccgg
     1201 cgagtcgcag ctcgctcatc gccgggcggc tcgtccaggg agccatcgtc ggcgtcatcc
     1261 agtcggccat catcgtcgtg ctcggcttca tcgtgggcgc cgagtacccc ggcggcatcg
     1321 ccggcatcgc cgcgctcacg ctcggcgccg tgctcctcgg gaccggcgtc ggcgccctca
     1381 gcaatgccgt gggcgtcctc tcccgccgcg tcgagacgat gatcgccgtg agcaacttcg
     1441 tgctgctccc gctgacgttc ctgagctcgg tgttcatggc gcgcgcgctc atgcccgggt
     1501 ggatgcagag cgtcgcgacc tacaacccgg tggagtgggc ggtcggcgcc gggcgggagg
     1561 cgctcgccgc gagccccgac tggtccgtcg tgctggcgcg gctgggctgg ctggccctgt
     1621 tcacgttcgt ctgcggctgg ctggccacgg cggcgttccg cgtctaccag aagtcgatct
     1681 gaggacgcgc gtcagagcgc cgggtagacc tcgatctccg cgatcctgca gagggatccc
     1741 cgcgtggtgc cgtcgcacga gacgcgcacc cagcgcgccg tgcgcggagc gaagtcgagc
     1801 gtctcgaaac ccgcccactc cccgctggtc agcgaccgct tcacgggccg ccagtgcctc
     1861 ccgtctccgg acaccaggat gcgcagcggg gcggcccggt cgtcgccgcg ggcgagggcg
     1921 acgcggaccg cgccgacctc gcggacgcct tgcagccgca ggtcgatgga ctggggaccg
     1981 cgcgccgccc agctcgttga gaggctgcca tcgatcgccg ccgaggggac gccggtggcg
     2041 gacctcgccg tgaccactgg gagcggcggc ttctcggggc tcaggcgccg caggcgcagc
     2101 agcgcctcga ggaagaaggc gtcgccgtac gcgagccctc ggtcggtgat gtccatccac
     2161 caggagtacg tgccgtgcag caggacggag ggcgtccacg tcgcggagct gtacgccggt
     2221 gacatcaagg aggcgagcgt ctcccgggcg gcgccggcgt acgccgcgcg ccgctcctcg
     2281 tcggggtcca gcagcgccag gtcgatcaga cccgatgccg cgatggccgc cgcggacgtg
     2341 tcccgcggcg ccgccgggat gccgggagcg ccgaagtccc agtacggcac catgtcgtcg
     2401 gggacgtggc gcagccacca gtcggagacc aggcgcgcga cctccaggaa cctcgggtcg
     2461 cgcgtctcac ggtacgcggc cgggaagccc agcaccgccc atgcctggcc gcgggcccag
     2521 gtggagccgt ccgcgtaggc ggcgcccttg tcgacccgct tcagcgcgcc ggtcgccggg
     2581 tcgtagaacg cggcgtggac cgtgctgccg tcgggccgga cgtggtcgcg cgcgatcgtc
     2641 agggcgtgcg agcgggcgat cgcgtcgtaa cgcgccggcc cgccggcctt gaccgcccac
     2701 cacaggagac gcggcttggt gaggctgtcg atgatgacgt tgaactcttc tccgtcccgg
     2761 gagcgcatcg cacccaccag cggatcgaag cgagccgcca tcgacctcga gacctgcaga
     2821 gatcgccggc gcaggccgtc cctgccggtc agcttgtagc cacgcacgta gctggggaag
     2881 aagagcccgc cgaggttctg tgactccggt ccgatgtcct cggcgccgag ccacgactgc
     2941 ctctgcagcg ccctgtcgcg ccatcggccc gcgcccgtcc actggtacat cgaccagagc
     3001 gatccggggt ggaagccggc cgcccagcct gagggaccgc tgtagtgcca gccgtcgccc
     3061 ctggtgtagt aggcgaaccg gcgcggcccg gcgcggcgat ccatcgccgc gaggcgagcg
     3121 tcggcgcgct tcagcgtcgc gcgcaccctg ccggagaagt cgtccgggac cgcggcggcg
     3181 ggtgcggcgg cgatcaggag ggcgccgatc gcgagcgcca gggcgcacag cgcggcgacc
     3241 gccggtgcgg ccgtccccgc cagtctcgtc ggcatgtggt ccatcctgcg gcccctccgc
     3301 gcctgaggaa gatcgtgctc cgcggcagac tctcgcgcac ttccccgcgg ccggtcaagc
     3361 cgaaggaccg cgggccttcc ccggaatgcc ccacccgggt agacttgcgg gcgtcgggcc
     3421 gggccggcgg cagcgccgcc cggcggcagc gccaacacca gccggaggcc tcatgcgcgt
     3481 gctcgtggtg ggcggcggcg gcagagagca cgccctcgtc cattcgttcg cccgatcccc
     3541 gctcgcggac cgcatcttct gcgccccggg caacgccggc acggccgcgg aggccgacaa
     3601 catccccgtc gccgccgacg acctccccgc cctgctcgac ttcgccagcc gcgaggaggt
     3661 cgacctcacg gtgatcggcc ccgaggcccc gctcgtcctg ggcctcaccg acctcttcga
     3721 agagcacggc ctcgcggtct tcggcccgaa cagggaggcc gcgcaactgg agggcagcaa
     3781 ggccttcgcc aaggagatca tgtccgacgg caaggtgccc accggcgcct accggcggca
     3841 cgagcggctg gagccggccc tgctcgacgt cgagggccgc gacgactacc cgctggtggt
     3901 caaggccgac ggcctcgccg ccggcaaggg cgtgctcatc tgcaaggacg tggacgaggc
     3961 ccgcgaggcc gtccatgcct gcttcgtcgc gcgcgagttc ggcgcggccg gcgacgtgct
     4021 catcaccgag gagtacctca ccggctccga ggtctcgctg ctggtgctct gcgacgggtc
     4081 gacggcggtg ccgctcgccc cggcgcagga ctacaagcgc atcttcgccg gggaccaggg
     4141 gcccaacacc ggaggcatgg gcagctactg ccccgtccca gggttcgagc agaaggccat
     4201 cgacgccgcg atggagcacg tggtcgagcc ggtcgtcgcg gcgctgcgac gccgcggcgt
     4261 cacctaccgc ggggtcctgt acgccggcct catcgtcacc gacgccggca tcaaggtgct
     4321 cgagttcaac tgtcgcttcg gcgaccccga gacgcaggcc gtgctgccgc ggctgagcag
     4381 cgatctgctc gagcattgcg ccgcggcggc gaacggcgac ctcgagagca ccgaggtcct
     4441 gtggacgccg caggactgcg tcaccgtcgt gctcgcctcc gccggctacc cggcgagcag
     4501 ccacaagggc gacgtcatcg gcggcctggc cgaggcggcc gccctcgaag gcgtgaccgt
     4561 ctaccacgcc ggcacggcgc tgcgggacgg cgaagtggtc acggccggcg gccgcgtgct
     4621 cgcggtgagc gcgctcggag acggcttctc cgccgcccga gcgcgcgctt atgagggcgt
     4681 cgccaggatc ggcttcgagg gcatgcaggt acgtcccgac atcgccgagc gcgccgtcaa
     4741 ggcgcgtgcc ggcgagctcg atctgttccc ggccgggttc gccggctgac cgaccatctg
     4801 ggcggcgcga gtctctccgc cgccggtgaa ggagacgacg tggaagagac gcagaccgag
     4861 gtcgagacga tccttcaggg catccagatg gagcccgtcc tggtcggcat cgtcatgggc
     4921 agcgagagcg accgcgaggt gatggacaag gccgcggccg agctcgacga gcgcaagatc
     4981 aggtacgaga tcagcgtcct ctcggcgcac cgcgatccgg agaaggtagc ccactacgcc
     5041 aagaccgcgc agagccgcgg gctcaaggtg ctcatcggcg gcgccggcaa ggcggcggcc
     5101 ctgccggggg tgctggccgc ctacaccaat cttcccgtga tcggcgtgcc catcaagacg
     5161 agcgacctgg gcggcctcga ctcgctgctc agcatcgtgc agatgccgcc cggcgtgccg
     5221 gtggcgtgcg tcgccatcaa cggcgcccgc aacgcggcca tcctcgccgc gaagatcctc
     5281 gacctctgac cgcggcaggc gggcgggcgg ttcccgtccg caccgcctgg tgcctccccg
     5341 ccgcgcacgg ggaacactca tgccatgggc aggcacgcga tcaggtccgc cgtggcgccg
     5401 ccgccgctcg gcgccgcctc ttccggcatc tcgtcgggac acttcgtctt cgtctccgcg
     5461 cagctcccgc tcgacgccgc cggcgagatc gcgggcgcga cggcggccga gcaggccgac
     5521 cgcgcgctcc agaacctggc ccaccagttg cggtccaccg gcctttccct ggaggacgtc
     5581 gtcagcctgg agatccacct cgtcgaccag gcggacaggg cgtccgtcga cgccgcactc
     5641 gaggctcggt tcctgccgcc gtatcccgcc gtcacgctct ccggtgtacc atggttgccc
     5701 ggcggtgcgc ggctgcaggt cgccgccatc gcacaacgct actgagagtc gcagggtgcc
     5761 gaggaacgac aagccgccca gcccgaggac catctcgggc ttcctctact tcgcggtgct
     5821 cttcatcgcc gtcgcgctgg cctacgtggc cgtgcgcgcg ctgctcgcct gacgccaggc
     5881 ccacacgaaa ggacaccccc atggagaagc aggtcttcgc gtcccccacc ggcgcccccg
     5941 ccgtcggccc gtactcgccc gccgtgggcg ccggcgactt cgtcttcgtc tccggccaga
     6001 tcccgctgga ccccgacggc aggatcgtcg gctacacgcc caaggaccag gcgcgcaagg
     6061 ccctcgagaa cctgcgcgcc accctccagg ccgcaggact gggcctcgag cacgtggtca
     6121 agacgaccat cttcctcagg gacatggacg agttcgccgc cgtgaacgag gtgtacggcg
     6181 agttcttcca ggagccgtac ccggcgcgct ccacagtcga agtggcccgc ctccccaagg
     6241 agctccacgt cgagatcgag gccgtggcgt accgtgggtg acgcgcaccg ccgcactccg
     6301 cgacgcgcgg cgcgcgcact cgccgtcctc ctgacagcgc tcgccgcgct gggcgcggga
     6361 gcgctcgccg gctgcgggag cccggccgag aacgacccct tcgtcggcta ctgggtcggc
     6421 ggcggcatgc aaggccagat gacgctggtg cagatcggca aggacgagga cggcaagtac
     6481 gtcgtgctgg ccaacccgga ccagccggcc ggcgaggccg tgcgggaggg agacagcctc
     6541 gtcgtcgaca ctcacgccgt ggtgatgcgt ttcacgccgg cgcccgacga caggctgagc
     6601 ctggagttct ccggtgacat gttcgaggag ccggcggcga ccacgctgaa gcgcaccgat
     6661 gcgacgcagt acagcgacgc cgcggtgggg tacgggctcg cccagatcga gcgcggcctg
     6721 gcgatgtgga aggggggcgg cggcaagaag tacccgcctc ccaaggaagt gacgccttcg
     6781 gggctgctcg gccagatgat ccgctggccc aacaacccgt tcaccggcca gcccatgcag
     6841 cccgggcaga gcgagggcga ctacgtgtac aagcaggtcg ccggcggcaa gaagtactcg
     6901 ctcaaggggt acctctcgga cggcagcacc atcggggagt gacggccggc cggcgaagcg
     6961 tcgccggccg tc
//
LOCUS       DASUMX010000343         7992 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_856646, whole genome shotgun sequence.
ACCESSION   DASUMX010000343 DASUMX010000000
VERSION     DASUMX010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 7992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7992
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_856646"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1566)
                     /locus_tag="VFZ86_16000"
     CDS             complement(<1..1566)
                     /locus_tag="VFZ86_16000"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX5643815.1"
                     /translation="MAEELAFARKASGLVRGLSMWDAFGVGFMNQGLTPSIWVMTSLG
                     VGVYLGGNLIWATIISMILAGIGFPIVWGVLGGSMPRSGGEYIYNSRIIHPIVGIGES
                     FGNAFVWIMWIYVLAPWTADPGMVMMSQFMNWTWLYNPETEQFFGSLSYSWGAFVIAT
                     VANIIAFLFVVFGIKWFARIQKVVMIFGIGGCTVLLAAISYYALKIGQDGFIESWNAT
                     AATYGSIDYAGFLAAAGEEAGGAMPTTWNWFDTFGVMVAGSWLFAYSYCITFIAGEVK
                     RPDKTIILSNLFAIIVPGVFMLWAAIALYGLVKFDFFSATQFVDNAGSDLGGAYTVPW
                     STNFLGILGMVTTNKLIMFISVLSFLAFNLWWIALSYLAFPRIIFAWGMDRMGPKWFT
                     DINPRFASPVKNHVLCFVLGQIMIAVYIFWQNEAMQGLAVTALEIFSVFGVTAIAALL
                     FPYVKRARGIWDASPYKTWKIAGIPVVTIGAIVDLIYLGILAYFFFFLPDPAKRLEGF
                     TANTAILIGVTWAA"
     gene            complement(1653..2144)
                     /locus_tag="VFZ86_16005"
     CDS             complement(1653..2144)
                     /locus_tag="VFZ86_16005"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX5643816.1"
                     /translation="MPFTLDAIDRQIVSLLIEDGRASSAEITRRIGHVSERAVRYRIE
                     RLIRTGVIRVSAIVMPQAVGFPVIGDVLVDVAPGKLQDVAAQLVEFPTVSYVAGSVGE
                     GDLSVQVYARDTEELLRFVNEVIGKVPGVTRTKLAMVPWKLKDVYQWHVPSEAAEEGR
                     AIG"
     gene            complement(2479..4047)
                     /locus_tag="VFZ86_16010"
     CDS             complement(2479..4047)
                     /locus_tag="VFZ86_16010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643817.1"
                     /translation="MTDAVAVGMMNQGITPSIWTMVTWGLGTYLTGNLLWASVISLVL
                     VGFGFPLVWGILSGSMPRSGGEYIYNSRIIHPIVAIAESFGNAFVMIFWVYILAPWVA
                     DPGLVIFAEYQNWTWLYDSATGAFFGGLSYDWGVFVVATITNIIAFFTVVFGMKFFAR
                     IQKVIMVLALGGAAVVTASIMITSKTAFAEKWDALATQYDSLKYSEVVPAVSAAIGEA
                     VPTSSTFYGTLGVMVAGSWLFAYAYFCVFVAGEVKRPDKTLITANFIAILIPALFMIW
                     TALSMYRAMPFDFISSTAWIDNNGGIEGYNLPYLPSYMSLAYVANSNWFVALAASGSY
                     ILFCFWYVALSYLAFPRIIFAWGMDRMGPKWFTSINPRWASPVKNYILCFILCQGAIA
                     LYTFWLGDYMQGLTVTGMEIVSVWGVTTIAALIFPYVKRARGIWDSSPYKTWKFLGIP
                     LVTWGAAVSLVYLGILAYFLLFTEEMKDSNGQSWILYGVVWILGIVWYFFWAARSKSV
                     GVDVGMTYGELPPE"
     gene            complement(4349..6238)
                     /locus_tag="VFZ86_16015"
     CDS             complement(4349..6238)
                     /locus_tag="VFZ86_16015"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEX5643818.1"
                     /translation="MPEATVPAEGVRVGVAAGADGRRRVSVCAGTACVFAGSMKIHEA
                     FAAEIAAAGLQDQVEVGIIGCHGLCSQGPLAVVSDGDTYYPRLKLKDVKTVVEEHLLG
                     GQVVDKLLYVDPATGERIACARDIPFYKAQTRIALRDVGVIDPESLDEYLARGGYEAA
                     RKALTTMTPEQIREEILDSGLRGRGGAGFPTGMKWKFAAASPGDVKYIICNGDEGDPG
                     AFMDASIMDGDPHAVLEGMLIGSYAIGAHEGYIYVRAEYPLAVKRLRLAIAAAEERGL
                     LGDDALGSGWSFHLKIKEGAGAFVCGEETALIASIEGKRGMPRTRPPFPAASGLWGKP
                     TNINNVETWANVPWIVANGAAAYAAYGNDTCKGTKAFSLAGKIVNGGLAEVPMGSTLR
                     HVIFDVGGGIKDGKKFKAVQLGGPSGGCVPASLLDTPVDYESLAATGAIVGSGGMVVA
                     DDTTCMVDLARYFLQFTQNESCGKCVPCRLGTKRMLEILERITAGEGEEGDVERLESM
                     ALNIKRTSLCGLGQTAPNPVLTTIRYFRDEYLAHIDDRRCPALSCEALIAYTIDPDAC
                     TGCTLCAKKCPVEAATGEKKQPHTIDQGLCIRCDACRVACKFDAVKIVSGPEEIAAAV
                     RAQHT"
     gene            complement(6231..6782)
                     /locus_tag="VFZ86_16020"
     CDS             complement(6231..6782)
                     /locus_tag="VFZ86_16020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014012578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEX5643819.1"
                     /translation="MPRREATPAPADAAGAAPEPDVDLSRVAELVAEIDPRSNLIALL
                     QRAQDAYGYLPEPVVDEIARLSGIPASRLYGIITFYAQFTTVPTGRHKCFVCHGTACH
                     VAGATRITEALEQELLVADGETRADREVGLDSVACMGACSQAPVMRIDDETYGNLSAD
                     ETRKIARKLLAQIAAEQGGGSDA"
     gene            complement(6894..7748)
                     /locus_tag="VFZ86_16025"
     CDS             complement(6894..7748)
                     /locus_tag="VFZ86_16025"
                     /inference="COORDINATES: protein motif:HMM:NF014881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HEX5643820.1"
                     /translation="MAAQIIDGKAIAEQVREEIKAEVARLATCGVKPGVATILVGEDG
                     GAQFYRGQIEKNTATVGFEYFNHMLPAGTGEAEILDLVAKLNADPAVHGILALMPMDA
                     QIDQNTVFNAIAPEKDLDCVNPANIGHVLLGDQLFAPATPSACIEILDREGIELQGLD
                     VTIVNHSNIVGKPVALLCLNRNASVHVVHVFSKDVPAACRQADVLISAAPVQNLIKAD
                     MVKPGAVVVDVTTIRVDGKQVGSLEFEAVCEVAGRVTPVPGGVGPVTNVILLKNALVA
                     CKAQTGVK"
     gene            complement(7783..>7992)
                     /locus_tag="VFZ86_16030"
     CDS             complement(7783..>7992)
                     /locus_tag="VFZ86_16030"
                     /inference="COORDINATES: protein motif:HMM:NF016825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HEX5643821.1"
                     /translation="GNPNAVTDAGVGVLLAEAAVVGAALNVKINLGSIDDAEFVAATA
                     AQVETLQAEAAALRAEVLAITLSKL"
BASE COUNT         1329 a         2586 c         2794 g         1283 t
ORIGIN      
        1 cgcggcccac gtcacgccga tgaggatcgc cgtgttcgcg gtgaagccct cgaggcgctt
       61 ggccgggtcg ggaaggaaga agaagaagta cgccaggatg cccaggtaga tcaggtcgac
      121 gatcgcgccg atcgtgacca cggggatgcc ggcgatcttc caggtcttgt acggcgacgc
      181 gtcccagatc cccctggcgc gcttcacgta ggggaacagc agggccgcga tggccgtgac
      241 gccgaacacc gagaagatct ccagcgccgt gaccgccagc ccctgcatgg cctcgttctg
      301 ccagaagatg tacaccgcga tcatgatctg gccgaggacg aagcacagga cgtggttctt
      361 gaccgggctc gcgaagcgcg ggttgatgtc cgtgaaccac ttggggccca tgcggtccat
      421 gccccaggcg aagatgatgc gcgggaacgc caggtagctg agggcgatcc accagaggtt
      481 gaacgccagg aacgagagta ccgagatgaa catgatgagc ttgttcgtcg tgaccatgcc
      541 gaggattccg aggaagttcg tgctccacgg cacggtgtac gccccgccca ggtcgctgcc
      601 ggcgttgtcg acgaactgcg tcgccgagaa gaagtcgaac ttgacgaggc cgtacagcgc
      661 gatggcggcc cagagcatga acacgccggg gacgatgatc gcgaacaggt tggagaggat
      721 gatggtcttg tccgggcgct tgacctcgcc ggcgatgaac gtgatgcagt agctgtaggc
      781 gaagagccac gagccggcga ccatgacgcc gaaggtgtcg aaccagttcc acgtggtcgg
      841 catggcgccg ccggcctctt cgcctgccgc cgcaaggaag ccggcgtagt cgatggagcc
      901 gtaggtggcg gcggtcgcgt tccagctctc gatgaacccg tcctgaccga tcttcagcgc
      961 gtagtagctg atcgccgcga gaaggaccgt acagccgccg atcccgaaga tcatgacgac
     1021 cttctggatg cgcgcgaacc acttgatgcc gaacaccacg aacaggaagg cgatgatgtt
     1081 ggccaccgtg gcgatgacga aggcgcccca cgagtagctc agggacccga agaactgctc
     1141 ggtctccggg ttgtagagcc acgtccagtt catgaactgg gacatcatca ccatgcccgg
     1201 gtcggccgtc cacggcgcca gcacgtagat ccacatgatc cacacgaagg cgttgccgaa
     1261 cgactcgccg atacccacga tggggtggat gatgcgcgag ttgtagatgt actcgccgcc
     1321 cgacctgggc atggagccgc cgagcacgcc ccagacgatc gggaagccga tgccggcgag
     1381 gatcatggag atgatcgtcg cccagatgag gttgccgccc aggtagacgc cgacgccgag
     1441 gctggtcatg acccagatcg acggcgtgag cccctggttc atgaagccga cgccgaaggc
     1501 gtcccacatg gagaggccgc ggacgaggcc gctggctttg cgggcaaagg ctagttcttc
     1561 agccaaagtt ccccccttgc tacagtgtct cgccggtcat ggcccccggc cggcgaaccc
     1621 gtgattgacg gtgcaacgcg acgcgcggat cctcagccga tcgccctccc ttcttcagcg
     1681 gcctcggacg gcacgtgcca ctgatagaca tccttgagct tccacggcac catcgcgagc
     1741 ttggtgcgcg tcactccagg gaccttgccg atgacttcat tgacgaagcg gaggagctcc
     1801 tcggtgtcgc gggcgtagac ctggacactg aggtcgcctt caccgaccga ccccgccacg
     1861 tagctcacgg tgggaaactc gaccagttgt gcggcgacgt cctgcagctt gccgggggcg
     1921 acgtcgacga ggacgtcgcc gatgacggga aagccgacgg cctggggcat gacgatggcg
     1981 ctgacgcgga tgacgcccgt gcggatgagg cgctcgatcc ggtagcggac ggcgcgctcc
     2041 gacacgtgcc cgatgcgccg ggtgatctcg gccgacgagg cgcggccgtc ctcgatgaga
     2101 agggacacga tctgcctgtc gatcgcgtcg agggtgaatg gcaactggtc cccccctggt
     2161 ccggaccatt cgtagcgcta tttcgatacc tcgcgtcgga aaatgtcaag ggccgcaacg
     2221 gacgcgtagg attcggcagc cggcggaatg cgggtctttc cgaatctcac agttctgcac
     2281 gattcgcacc agacgcccat cgaatccgct tcgaccagcc tgtttcggac gttcaaccga
     2341 ggtcgtaagc gcctgaacgc gcggccggcg cggtccgagc cgggatcggc gcaaggaacg
     2401 tccggggcgg cgcgccgatc ggcgcgccgc cccggacgag tcacgtcgcc ggcagggcga
     2461 cgaagctgcc tgagcggatc actccggcgg cagctccccg taggtcatgc cgacgtccac
     2521 gccgacgctc ttgctgcgcg cggcccagaa gaagtaccag acgatgccga ggatccagac
     2581 gacgccgtag aggatccacg actggccgtt cgagtccttc atctcctcgg tgaagagcag
     2641 gaagtaggcg aggatgccga ggtacacgag ggagaccgcg gcgccccagg tcacgagcgg
     2701 gatgccgagg aacttccacg tcttgtacgg cgacgaatcc cagatgcccc tcgcgcgctt
     2761 cacgtacggg aagatcagcg cggcgatggt ggtgacgccc cacaccgaga cgatctccat
     2821 gccggtgacc gtgaggccct gcatgtagtc gccgagccag aaggtgtaga gggcgatggc
     2881 tccctggcag aggatgaagc acaggatgta gttcttgacc gggctcgccc agcgcgggtt
     2941 gatcgacgtg aaccacttgg gccccatgcg gtccatgccc caggcgaaga tgatgcgcgg
     3001 gaaggcgagg tagctcagcg ccacgtacca gaagcagaac aggatgtacg agccgctcgc
     3061 cgccagggcg acgaaccagt tcgagttggc cacgtaggcc aggctcatgt agctgggcag
     3121 gtacggcagg ttgtagccct cgatgccgcc gttgttgtcg atccacgccg ttgacgagat
     3181 gaagtcgaac ggcatggcgc ggtacatcga gagcgcggtc cagatcatga acagcgccgg
     3241 gatcaggatg gcgatgaagt tggccgtgat gagcgtcttg tccgggcgct tgacctcgcc
     3301 ggcgacgaag acgcagaagt aggcgtaggc gaacagccac gagccggcca ccatcacgcc
     3361 gagggtcccg tagaacgtcg aggacgtggg gaccgcctcg ccgatggccg ccgacaccgc
     3421 cggcaccacc tcggagtact tgagcgagtc gtactgggtg gcgagggcgt cccacttctc
     3481 agcgaacgcc gtcttgctgg tgatcatgat cgatgcggtc acgacggccg cgccgccgag
     3541 ggcgagcacc atgatcacct tctggatcct ggcgaagaac ttcatgccga acacgacggt
     3601 gaagaaggcg atgatgttgg tgatcgtggc aacgacgaac acgccccagt cgtaggagag
     3661 gccgccgaag aaggccccgg tggcgctgtc gtacaaccac gtccagttct ggtactcggc
     3721 gaagatcacg agcccggggt cggcgaccca cggcgccagg atgtagaccc agaagatcat
     3781 cacgaaggcg ttgccgaagc tctccgcgat ggccacgatc gggtggatga tgcgcgagtt
     3841 gtagatgtac tcgccgcccg agcgcggcat gctgccggag aggatgcccc acacgagcgg
     3901 gaagccgaat ccgacgagca ccagcgagat gacgctggcc cagagcaggt tgccggtcag
     3961 gtaggtgccg agcccccagg tgaccatcgt ccagatgctc ggcgtgatgc cctggttcat
     4021 catgccgacg gcgacggcgt cggtcatcga cagaccacgt accaggccgc tggctttgcg
     4081 tgcgaatgcg agttcttctg ccacactacc cccctagtct gcggacgccc ggccggcccc
     4141 tggcggcgcg cccctgtccg tagagcccaa agatcgcccc tgtgccatcc accccctcgt
     4201 ggtcggtcga cagactgact tggttagtca cacaacgaac gatacgcgca tcacgcggcc
     4261 cggggcaagg ggagaggtaa aagagagagg ggccggcgac gccagtcgcg gcatcgccgg
     4321 cccctctcgc tgtgcatctt cgtgcgcgtc aggtgtgctg cgcgcgcacc gcggcggcga
     4381 tctcctcggg tccgctcacg atcttgaccg cgtcgaactt gcaggcgacg cggcaggcgt
     4441 cgcagcggat gcagagcccc tggtcgatgg tgtgcggctg cttcttctcg ccggtcgcgg
     4501 cctcgaccgg gcacttcttg gcgcacagcg tgcagccggt gcaggcgtcc gggtcgatgg
     4561 tgtaggcgat gagcgcctcg cacgacagcg cggggcagcg gcggtcgtcg atgtgcgcca
     4621 ggtactcgtc gcggaagtag cggatcgtgg tcagcaccgg gttgggagcg gtctggccga
     4681 gcccgcagag gctcgtcctc ttgatgttca gcgccatgct ctcgaggcgc tccacatcgc
     4741 cctcctcgcc ctcgccggcg gtgatgcgct cgaggatctc cagcatgcgc ttggtgccca
     4801 ggcggcaggg cacgcacttg ccgcagctct cgttctgcgt gaactggagg aagtacctgg
     4861 cgaggtcgac catgcaggtg gtgtcgtcgg cgaccaccat gccgccggag ccgacgatgg
     4921 cgccggtcgc ggcgaggctc tcgtagtcga ccggcgtgtc gaggagcgag gcgggcacgc
     4981 agccgcccga cggtccgccg agctgcacgg ccttgaactt cttgccgtcc ttgatgccgc
     5041 ccccgacgtc gaagatgacg tggcggaggg tcgagcccat gggcacctcg gcgaggcccc
     5101 cgttgacgat cttgccggcg aggctgaacg ccttggtgcc cttgcacgtg tcgttgccgt
     5161 aggcggcgta ggcagccgcc ccgttggcga cgatccaggg cacgttggcc cacgtctcga
     5221 cgttgttgat gttggtgggc ttgccccaca ggcccgacgc cgccgggaac ggcggccgcg
     5281 tgcgcggcat gccgcgctta ccctcgatgg aggcgatgag cgcggtctcc tcgccgcaga
     5341 cgaaggcgcc ggcgccttcc ttgatcttga ggtggaagct ccagccgctg ccgagcgcgt
     5401 cgtcgcccag caggccgcgc tcctcggcgg ccgcgatcgc cagacgcagg cgcttgacgg
     5461 cgagcgggta ctcggcgcgc acgtagatgt agccctcgtg cgcgccgatc gcgtagctgc
     5521 cgatgagcat gccctcgagc acggcgtgcg ggtcgccgtc catgatggag gcgtccatga
     5581 acgcgccggg gtcgccctcg tcgccgttgc agatgatgta cttcacgtca cccggcgaag
     5641 cggcggcgaa cttccacttc atgcccgtcg ggaagccggc gccgccgcgg ccgcgcagcc
     5701 ccgagtcgag gatctcctcg cggatctgct ccggcgtcat cgtcgtgagc gccttccgcg
     5761 ccgcctcgta tccgccacgg gcgaggtact cgtcgaggct ctccgggtcg atgacgccca
     5821 cgtcgcgcag cgcgatgcgc gtctgcgcct tgtagaacgg gatgtcgcgg gcgcaggcga
     5881 tgcgctcgcc ggtggcgggg tccacgtaca ggagcttgtc gacgacctgg ccgccgagga
     5941 ggtgctcctc gacgacggtc ttgacgtcct tgagcttgag tcgcgggtag tacgtgtcgc
     6001 cgtccgacac gacggcgagc ggaccctggg agcacaggcc gtgacagccg atgatgccga
     6061 cctcgacctg gtcctggagg ccggcggcgg cgatctcggc cgcgaacgcc tcgtggatct
     6121 tcatggaccc ggcgaagacg caggcggtgc cggcacagac ggagacgcgc cggcggccgt
     6181 cggcgccggc ggcgacgccg accctgacgc cctcggcggg cacggtggcc tcaggcatcg
     6241 gagcctcctc cctgctccgc cgcgatctgc gccaggagct tgcgcgcgat cttgcgcgtc
     6301 tcgtcggcgc tgaggttgcc gtaggtctcg tcgtcgatgc gcatgacggg cgcctgcgag
     6361 caggccccca tgcaggcgac ggagtccaga ccgacctcgc ggtcggcacg cgtctcgccg
     6421 tcggccacga ggagctcctg ctccagcgcc tcggtgatcc gcgtcgcgcc ggccacgtgg
     6481 caggcggtgc cgtggcagac gaagcacttg tggcgcccgg tgggcaccgt ggtgaactgc
     6541 gcgtagaagg tgatgatccc gtagaggcgc gaggcgggga tgccgctgag cctggcgatc
     6601 tcgtcgacga ccggctccgg gaggtagccg taggcgtcct gagcgcgctg cagcagggct
     6661 atcaggttgc tgcgcgggtc gatctccgcg acgagctcgg cgaccctgga gaggtcgacg
     6721 tctggctctg gggctgcgcc ggccgcgtcg gccggcgccg gggtggcttc tcgcctgggc
     6781 atcgcatcaa ctccggttct cgtgcctgag ctcctagtga accacacttg gcgggcggca
     6841 ggtaccgcgg ccccgaggcc gcagcgcccg ccgcccgccg gcgtccggat cggctacttg
     6901 acgcccgtct gcgccttgca ggcgacgagg gcgttcttga gcaggatgac gttggtgacc
     6961 gggccgacac cgccgggcac cggcgtgacg cggccggcga cctcgcacac ggcctcgaac
     7021 tccagcgagc cgacctgctt gccgtccacg cggatcgtgg tcacgtcgac gaccaccgct
     7081 cccggcttga ccatgtcggc cttgatcagg ttctgcacgg gagccgcgct gatgagcacg
     7141 tcggcctggc ggcaggcggc gggcacgtcc ttgctgaaca cgtgcacgac gtggaccgag
     7201 gcgttgcggt tgaggcagag cagcgcgacg ggcttcccga cgatgttcga gtggttgacg
     7261 atcgtgacgt cgaggccctg cagctcgatg ccctcgcggt cgaggatctc gatgcaggcg
     7321 ctcggcgtgg ccggcgcgaa cagctggtcg ccgagcagca cgtggccgat gttggccggg
     7381 ttcacgcagt cgaggtcctt ctcgggagcg atcgcgttga acacggtgtt ctggtcgatc
     7441 tgcgcgtcca tgggcatgag cgcgaggatg ccgtgcacgg ccggatcggc gttgagcttg
     7501 gcgacgaggt cgaggatctc ggcctcgccg gtgccggccg ggagcatgtg gttgaagtac
     7561 tcgaagccca ccgtggcggt gttcttctcg atctggccgc ggtagaactg cgcgccgccg
     7621 tcctcgccca ccaggatggt ggcgacgccg ggtttgacgc cgcaggtcgc gagcctggcg
     7681 acctcggcct tgatctcttc gcggacctgc tcggcgatgg ccttgccgtc gatgatctga
     7741 gctgccatgg cttccctctc gtgtgtggtc agggggtgat gatcagagct tggagagcgt
     7801 gatcgcgagg acctcggcgc gcagcgccgc ggcctcggcc tgcagcgtct ccacttgcgc
     7861 tgcggtcgcg gcgacgaact cggcgtcgtc gatcgaccca aggttgatct tgacgttcag
     7921 cgccgcgccg acgacggccg cctcggccag cagcacgccg acgccggcat ccgtcacggc
     7981 gttcggattg cc
//
LOCUS       DASUMX010000344         2011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_568337, whole genome shotgun sequence.
ACCESSION   DASUMX010000344 DASUMX010000000
VERSION     DASUMX010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568337"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..943
                     /locus_tag="VFZ86_16035"
     CDS             <1..943
                     /locus_tag="VFZ86_16035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014190603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5643822.1"
                     /translation="RLIGRRLLLMIPTLILASMLIFALAEVLPGDVGRSILGPYATEE
                     QVQLLNEKLGADRPLVVRYVAWAGGFVTGDWGESALLEVPVRPLVLDAFWNSLLLAGF
                     ALILIVPTSVALGVFAGLRRDSVLDRTITVSSLSMTVIPEFVSGVVLLYIFAVWLKWL
                     PVSAMPPEGSPFYERLYYLILPAIPLMFLELGYIARMARVGTVQVLSMPYIRTAVLKG
                     LPRRRVIFGHVLRNAMVPTVTVIGSQVGWLIGGLVVIEVLFVYPGIGKLMVDAALSHD
                     VPLLEASVLMVAIVYMLANLIADIVVALLNPRIRMGG"
     gene            945..1964
                     /locus_tag="VFZ86_16040"
     CDS             945..1964
                     /locus_tag="VFZ86_16040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007745287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5643823.1"
                     /translation="MNDERETPFGPDPDLRAETMELGADPEMIELRAEAAAARREAGP
                     AGGSPAQDGELRADEAAAAEQRRQRRYLIKALLRSPTFMIGLIIVIFWVLMALFSNAL
                     FQSFTAQDAANSLQAPSSAHWFGTDDLGRDVFARTMAGARSVLIIAPLATLLALLWGG
                     IVGLISGYYRGITDEIIMRAIDVLLALPIIITSILILSLFGKGTAIIIIAIGALFTPV
                     VSRTVRAAVIGEREREYVMAARLRGERSAFVMGREILPNITQPIIVEGTIRLGYAVFT
                     AATLSFLGFGLVPPSPDWGLTIANERIFLQIAPWTVMFPAAALASLVVAVNLITDGLG
                     RTFSK"
BASE COUNT          278 a          735 c          627 g          371 t
ORIGIN      
        1 gcgactcatc ggacgccggc tcctcctcat gatcccgacg ctgatcctgg cgtcgatgct
       61 gatcttcgcg cttgcggagg tgctgcccgg cgacgtggga cgctccatcc tggggcccta
      121 cgcgactgaa gaacaggtcc aactgctcaa cgagaagctc ggcgccgacc ggccgctggt
      181 cgtgcgctac gtggcgtggg caggcggctt cgtcaccggc gattggggcg aatcagccct
      241 gctggaggtg ccggtgcggc cactggtcct cgacgccttc tggaactccc tcctcctcgc
      301 cggcttcgcg ctcatcctta tcgtgcccac gtcggtcgcg ctcggcgtgt tcgccggtct
      361 ccgaagagac agcgtcctgg accgcacgat cacggtctcc tcgctgtcga tgacggtcat
      421 tcccgagttc gtcagcgggg tcgtgctcct ctacatcttc gcggtgtggc tcaagtggct
      481 gcccgtctcg gcgatgccac cggagggctc gccgttctac gaacgcctgt actacctcat
      541 cctgccggcc atcccgctca tgttcctgga gctggggtac atcgcgcgca tggcgcgcgt
      601 cggcaccgtc caggtgctgt ccatgccgta catccgcacg gccgtcctca aaggcctgcc
      661 gcgtcgccgc gtcattttcg ggcacgtgct gcgtaacgcc atggtcccca ccgtcaccgt
      721 catcggcagc caggtcggct ggctgatcgg cggcctggtc gtcatcgagg tcttgttcgt
      781 ctaccccggc atcggcaagc tcatggtcga cgccgctctc tctcacgacg tcccgctgct
      841 cgaggcctcg gtcctcatgg tggccatcgt ctacatgctg gccaacctga tcgccgacat
      901 cgtggtcgcg ctcctcaacc cacgcatccg gatgggtggc tgacgtgaac gacgaacgcg
      961 agactccgtt cggtcccgat ccggacctgc gtgcggagac catggaactc ggcgcggatc
     1021 ccgagatgat cgagctccgc gcggaggcgg ctgcggcccg cagagaagcc gggccggccg
     1081 gagggagccc ggcccaggac ggcgagcttc gggccgacga ggccgccgca gccgagcagc
     1141 ggcgccagcg ccggtacctc atcaaggcgc tgctgcgcag cccgacgttc atgatcggcc
     1201 tcatcatcgt gatcttctgg gtgctcatgg cgctcttctc caacgccctc ttccagagct
     1261 tcacggccca ggacgccgcg aactccctgc aggcgccgag ctcggcccac tggttcggca
     1321 ccgacgacct cggacgcgac gtgttcgcgc gcacgatggc cggtgcgcgc tcggtgctca
     1381 tcatcgcgcc gctggccacg ctgctcgccc tgctctgggg cggcatcgtc ggcctcatct
     1441 cgggctacta ccggggcatc accgacgaga tcatcatgcg cgccatcgac gtgctcctcg
     1501 cgctgccgat catcatcacc tcgatcctga tcctctcgct gttcggcaag gggacggcga
     1561 tcatcatcat cgccatcggc gcactcttca ccccggtcgt gtcccgcacc gtcagggcgg
     1621 cggtcatcgg cgagcgcgag cgcgagtacg tgatggcggc gcgcctccgc ggtgagcgct
     1681 ccgccttcgt catgggccgc gagatcctgc ccaacatcac gcagcccatc atcgtcgagg
     1741 gcaccatccg gctcggctac gcggtgttca ccgccgccac gttgtcgttc ctggggttcg
     1801 gcctggtgcc gccgtcgccc gactggggtc tcaccatcgc caacgagcgc atcttcctgc
     1861 agatcgcccc gtggaccgtg atgttccccg cggccgcact ggcctctctc gtcgtggccg
     1921 tgaacctcat caccgacgga ctggggagga cgttctccaa atgagcagcg tggagaatca
     1981 gcagccggcc atgaccgacg ccgccgccct c
//
LOCUS       DASUMX010000345         2855 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_139280, whole genome shotgun sequence.
ACCESSION   DASUMX010000345 DASUMX010000000
VERSION     DASUMX010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139280"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="VFZ86_16045"
     CDS             complement(<1..252)
                     /locus_tag="VFZ86_16045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643824.1"
                     /translation="MRVSRKTLTLLLLAVLLVTFAVIAPAAPAKTLAPNAFGAKAYTF
                     VDQLTRITNADGTYTGLDRAAGTESEVVAAEKVAGWFGDA"
     gene            complement(313..738)
                     /locus_tag="VFZ86_16050"
     CDS             complement(313..738)
                     /locus_tag="VFZ86_16050"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX5643825.1"
                     /translation="MSIEDNKAVAAAVVERVMNQGDVAAVEEYYAAGYVEHDVLPPAI
                     PPGLEGIRQLFGMLRAAFPDLRYDVDDVVAERDKVVQRVTGSGTMHGSLFGLPATGRT
                     ASWSEIHISRFDGGKVVEHWSVTDQLGMLQQLGLTAYAR"
     gene            complement(790..2466)
                     /locus_tag="VFZ86_16055"
     CDS             complement(790..2466)
                     /locus_tag="VFZ86_16055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX5643826.1"
                     /translation="MRRFLTPLAVALVLVALVLATQATIAVGSPGVVSKVLVRGAPIH
                     GANGMAINAQGRIYVGSVFGREIVIVNSRTGRIVDRLGPDDGFNGADDVTFGPDGSLY
                     WTDILAGKVGRLESNGTMTMQDVAPFVNPITFSADGRLFVAQAFFGAGLYELDPELAD
                     PPVLRMPGSAVPPFLDQFNGFDFGPDGKLYAPHPFLGTIVRIDVDTWTEEIVADGLPF
                     PVALKFNAKGKLYAALQNSGDVVRVFRATGKTRVVARLQPGLDNLAFDARDRMFVSHA
                     GNGRIWRILPSGQPRRLIRGGLVAPGGIAVMRYQEPAVHDRLFVADGWEIKKYDGRNG
                     RAEGVIRQSFTGPSIIAALTASSDGRYLILTSWLDAAVQVWDPVTSTEVGLWTDILGP
                     LNAIRFGEDLVIADLATSSVILQTPAGLRTTLAGPLVVPTGLAAKGGDLWAADWAAGV
                     VYQLVDDGVNLPAPVAVATGLNQPEGLAVDRDGTLLVVEAGAGRLTRIDPATGAKTLV
                     RGRLALGAPGAPGMPPTWFFNGVAVGGRGDIYVTGDKTDVVYRIKVVPTR"
BASE COUNT          426 a          984 c         1015 g          430 t
ORIGIN      
        1 ggcgtcgccg aaccagccgg cgaccttctc ggcggcgacc acttcactct ccgtgcccgc
       61 cgccctgtcg aggcccgtgt aggtcccgtc ggcattggtg atacgcgtga gctggtcgac
      121 gaaggtgtag gccttggccc cgaacgcgtt gggcgcgagc gtcttggccg gcgccgccgg
      181 ggcgatcacg gcgaacgtga ccagcaacac cgcgagcagc aacagggtca gggtcttccg
      241 ggatacgcgc atactgaagc acctccagcg taggtgacat gactcctcta ctatgccacc
      301 ggtgaacggg cgtcaacgcg cataggccgt cagcccgagt tgctgcagca tccccagctg
      361 gtccgtcacc gaccagtgct cgacgacctt gccgccgtcg aaccggctga tgtggatctc
      421 gctccacgac gccgtcctgc cggtggccgg caagccgaag agcgagccgt gcatcgtgcc
      481 gctgccggtc acccgctgga ccaccttgtc cctctccgcc accacgtcgt cgacgtcgta
      541 gcggaggtcg gggaaggcgg cgcggagcat gccgaacagc tgcctgatcc cctccagccc
      601 cggagggatc gcagggggaa ggacgtcgtg ctcgacgtac ccggcggcgt agtactcctc
      661 gacggcggcg acgtcgccct ggttcatgac gcgctcgacg acagcggcgg cgacggcctt
      721 gttgtcctcg atcgacatcg cccggcctcc ttcccctacg tcttgtctcc ttctgtccag
      781 tcctcccgcc taccgcgtcg gcacgacctt gatgcgatag acgacgtcgg tcttgtcgcc
      841 ggtcacgtag atgtcgccgc ggccgcccac cgcgacaccg ttgaagaacc aggtgggcgg
      901 catgccgggc gccccggggg cgccgagggc gagacggccg cggaccaggg tcttggcgcc
      961 ggtggccggg tcgatgcgcg tcaggcgccc ggccccggcc tccaccacga gcagcgtgcc
     1021 gtcgcggtcc acggccagcc cctcgggctg gttcaggccg gtcgccaccg cgacgggcgc
     1081 cggcaggttc acgccgtcgt cgacgagctg gtacaccacg ccggcggccc agtcggcggc
     1141 ccagaggtcg ccgcccttgg ccgcgagccc ggtgggcacg acgagcgggc cggcgagcgt
     1201 ggtgcgcagc cccgccggcg tctggaggat cacgctcgac gtcgcgaggt cggcgatgac
     1261 gaggtcctcc ccgaagcgga tggcgttcag cggtccgagg atgtcggtcc acaggccgac
     1321 ctcggtggac gtcacgggat cccagacctg cacggcggca tcaagccacg aggtcaggat
     1381 cagataccgg ccgtccgacg aggccgtcag cgccgcgatg atgctcggcc cggtgaacga
     1441 ctggcggatg acgccctcgg cgcgtccgtt gcggccgtcg tacttcttga tctcccagcc
     1501 gtcggccacg aacagcctgt cgtgcaccgc cggctcctgg tagcgcatca cggcgatgcc
     1561 gccgggggcg acgagcccgc cgcggatcag gcgccgcggc tgcccgctgg gcaggatccg
     1621 ccagatgcgc ccgttgccgg cgtgcgagac gaacatgcgg tcgcgcgcgt cgaaggcgag
     1681 gttgtcgaga ccgggctgga gtcgcgccac gacccgtgtc ttccccgtgg cgcggaagac
     1741 gcgcacgaca tcgcccgagt tctgcagcgc ggcgtacagc ttccccttcg cgttgaactt
     1801 gagagcgacc gggaagggca gcccgtcggc cacgatctcc tccgtccacg tgtcgacgtc
     1861 gatgcgcacg atggtgccga ggaacgggtg cggcgcgtag agcttgccgt cggggccgaa
     1921 gtcgaagccg ttgaactggt cgaggaacgg cgggaccgcc gacccgggca tgcggagcac
     1981 cggtgggtcg gcgagctcgg ggtcgagctc gtagaggccg gcgccgaaga aggcctgggc
     2041 gacgaacagg cgtccgtcgg cggagaaggt gatcgggttc acgaacggcg cgacgtcctg
     2101 catggtcatc gtgccgttgg attcgagccg gcctaccttg ccggcgagga tgtcggtcca
     2161 gtagagcgag ccgtcgggac cgaacgtgac gtcgtcggcg ccgttgaagc cgtcgtccgg
     2221 ccccaggcgg tcgacgatcc ggccggtgcg cgagttgacg atcacgatct cgcgcccgaa
     2281 cacggagccg acgtagatgc gcccctgggc gttgatggcc atgccgttgg cgccgtggat
     2341 cggcgcgccg cggacgagca ccttgctgac gacgccgggt gagcccacgg cgatcgtcgc
     2401 ctgcgtcgcg agaacgagtg cgaccaggac cagcgcgacc gcgagcggcg tgaggaagcg
     2461 tctcatgaag catccctcca gtgcagctga gaatgaggat cagtctggat caggcgcctg
     2521 gatcaggcgg gtgaggagtc tgcgtccatc cgaccacccc ctggcgggct ggtgtcgtcg
     2581 gggccttggc cacaccgcgg ctcatcgccc cgaggccgcg cgacttcggc gcgcggcctc
     2641 gctcatgcat ttcgccttcc ccctgcccga ggggacaaac ccgtttgcat gcaccgtccg
     2701 cgatcaggcg tcgaccgcct gtgctggtga gtggcacgcc ccgcgtgcgt acgcctctgt
     2761 tcttgcgatt cgtggtcccg gcgccaaggt caccgtggtt caaccgaccc ggggagagac
     2821 ggttgcgaga cggtcgagag cgcagagtgg agtca
//
LOCUS       DASUMX010000346         6026 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_427025, whole genome shotgun sequence.
ACCESSION   DASUMX010000346 DASUMX010000000
VERSION     DASUMX010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 6026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6026
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427025"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="VFZ86_16060"
     CDS             <1..397
                     /locus_tag="VFZ86_16060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013388686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HEX5643827.1"
                     /translation="GPSADASDRPAADVAVTAEAGVRAQARQIVGQPAGPGLAVGPAR
                     VVRTREDLAAFADGEILVCDAIGPEMTFIVPLAAGIVERRGGMLIHGAIIAREYGLPC
                     VTGVPRAAELIRTGDRVTVDGYLGIVIVG"
     gene            complement(412..1494)
                     /locus_tag="VFZ86_16065"
     CDS             complement(412..1494)
                     /locus_tag="VFZ86_16065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643828.1"
                     /translation="MKKRPGFLSATGIVAAVVVFLGAGAVTHLAGPELFSPGGLNAQT
                     SAGAPDTGRAGPAGGVASHAELDGDCGACHPAPWSGRTMADGCLDCHTGVAAELEAGD
                     GLHGGLAGDGQTPACGPCHPEHNGPHGALTEIDERAFRDAHAATGFSLQSHAETESGG
                     SFACADCHPAGYQGFDEAVCADCHAGIDADFMREHEDAFGKECLPCHDGSGAVTVDHD
                     RFAFKLTGEHAGVACDACHEGARSLRDYRQAPRGCYGCHADDDEHDGAYGRGCGDCHS
                     ARGWDDVTFDHAVFPLDHGSEEREPTCETCHPGGTATYTCYGCHEHTTGNVRGRHEGR
                     SLAELKDCVRCHEGGESGEGSEGGGD"
     gene            complement(1510..2100)
                     /locus_tag="VFZ86_16070"
     CDS             complement(1510..2100)
                     /locus_tag="VFZ86_16070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643829.1"
                     /translation="MSSARTGVTGQAEAGRGAGAGGAAARARGRELLWALAAIVAATA
                     LYAAAASQAGRLPAASGLVGHGFGIAGSVLMLMTATLYSLRKLRSDARWGSTAAWLRF
                     HMVTGLVGPYLVLLHTAGRFSGLAGLTMLLTVVVVASGLTGRYFYTRVPRAPEAAARR
                     RALATWHLVHLPLTWALFAAAIIHVIAALYYATLQR"
     gene            complement(2093..3682)
                     /locus_tag="VFZ86_16075"
     CDS             complement(2093..3682)
                     /locus_tag="VFZ86_16075"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5643830.1"
                     /translation="MGFFTADREKYRTLAGAWVVPELRRCVQCGVCAFNCPMGLDVRR
                     HAWRGEPVGDSHCLTCGECAKRCPRGLLHFEPLGATGDGIAPGRRAGRPGTPRRHVLI
                     GGGPATIAAAEAIRGADAEAQIVVVGADPHGFYSRPGLAYYLTDELPERRLFPFSAAE
                     LRALGITWIGERAEAIEPGAHQVVLASGRRLEYDRLLLATGSTAIPAGVPGAGLDGVV
                     KLDNMDDARDIIRRSREANAAVVVGGGITALEIVEGMRARGARVHYFMRKDRYWSNVL
                     SASESTIVERRLRREGVEIHPFTDLAGIAGRGGKVSGVQTQDGATIPCDLVAVAVGVR
                     PKVELARAAGLPCARGVLVDEYLRAGDPDIYAAGDIAEIADPESGRGVLNVLWNVAVA
                     KGTVAGLNMATEPRQVYAEDRPLNITRLAGLHTTIIGTVGNGGDADLQGLSRGDSQVW
                     SELTESDVVEVERGEAHVRVALGERTIDGAVVMGDQALSYALQELVEARVDLSTAERV
                     IATPLAEVICNARADWGACRV"
     gene            3833..4303
                     /locus_tag="VFZ86_16080"
     CDS             3833..4303
                     /locus_tag="VFZ86_16080"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX5643831.1"
                     /translation="MTRIERSVHIEEKPEVVFRILTDLKLIPRWAVIAQDTYVRPESM
                     LTEAQQTFRWTIRIAGIDLETDWTVTEFDPPRAVTYAVESPHDGRLEMKQRVLDAGDG
                     GSRVELEVDYDLPYGFLGEALDKVVVEHRNQAVVERSLRNLKDLVEAIRDYAAA"
     gene            complement(4306..5271)
                     /locus_tag="VFZ86_16085"
     CDS             complement(4306..5271)
                     /locus_tag="VFZ86_16085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011204986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEX5643832.1"
                     /translation="MRDAANAPRGPVAVVGAGLTGASWAGLYAAAGLEVRLQDVDGER
                     LEAAVERAAAAAGFLAGSGLADMQAAERGVELLTATEDLAGAVAGVTHVQECVREDLA
                     LKREVFAAVGAAAPPDALICSSSSGLSISDIQTAAARPERCLAAHPYNPPHLVPLVEL
                     APGALTSPEAMERAAAFYAAVGKEPVKLTRDLPGYLANRLSAALWREAVDLVLRGVAT
                     VEDVDKAVSYGPGLRWAAMGPHLLYDLGGGEEGIVGHVEHLAAVKEGMLRDLATWTTF
                     PPETGEALDAGLAEEKGARTYDELVAERDALLVAYLRARRERLGQ"
     gene            complement(5421..5978)
                     /locus_tag="VFZ86_16090"
     CDS             complement(5421..5978)
                     /locus_tag="VFZ86_16090"
                     /inference="COORDINATES: protein motif:HMM:NF015996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HEX5643833.1"
                     /translation="MRNPYDMIGRLGLLLAIAGAVNWLLVGLFEWNFVQWLFTQSGTQ
                     TVGSLGERVVYIVVGVGGVLALPMLAATLARSRSRDVSSEERGRSTAGPSEDDTDFYY
                     GAPKNERELAREPQRVRRIERVTVEEPMPERSAATSAAETSAEATASAVGSEPRSPEE
                     RLRYGTVEETDEGLDEGYDERRRAA"
BASE COUNT          744 a         2251 c         2224 g          807 t
ORIGIN      
        1 cggcccgtcc gcggacgctt cggaccggcc ggccgcggac gtcgcggtca cggccgaggc
       61 cggcgtccgt gcccaggccc ggcagatcgt ggggcagccg gccgggccgg ggctcgcggt
      121 gggcccggcc cgggtggtgc gcacgcgtga ggatctcgcg gccttcgccg acggcgagat
      181 cctcgtctgc gacgcgatcg ggccggagat gaccttcatc gtgccgctgg ccgccggcat
      241 cgtcgagcgc cgcggcggca tgctgatcca cggcgcgatc atcgcccgcg agtacgggct
      301 gccgtgcgtc acgggcgtgc cgcgcgccgc ggagctcatc cgtaccggcg accgcgtcac
      361 cgtggacggc tacctcggta tcgtgatcgt cggctgaatc gctcgccggc gtcagtcgcc
      421 gccgccctcg ctgccctctc cgctctcgcc gccctcgtgg cagcgcacgc agtccttgag
      481 ctcggccaga gaccggccct cgtgccggcc gcgcacgttg ccggtggtgt gctcgtggca
      541 gccgtagcag gtgtaggtgg cggtgccgcc cgggtggcag gtctcgcagg tgggctcgcg
      601 ctcctcgctg ccgtggtcga gggggaagac cgcgtggtcg aaggtcacgt cgtcccagcc
      661 cctcgcggag tggcagtcgc cgcacccgcg gccgtaggcg ccgtcgtgct cgtcgtcgtc
      721 ggcgtggcag ccgtagcagc cccgaggcgc ctgccggtag tcccgcagcg agcgggcgcc
      781 ctcgtggcag gcgtcgcagg ccactcccgc gtgctcgccg gtcagcttga aggcgaacct
      841 gtcgtggtcg acggtgacgg cgccgctgcc gtcgtggcag ggcaggcact ccttgccgaa
      901 ggcgtcctcg tgctcccgca tgaagtcggc gtcgatgccg gcgtggcagt cggcgcagac
      961 ggcctcgtcg aacccctggt acccggcggg gtggcagtcg gcgcaggcga agctgccgcc
     1021 ggactcggtc tcggcgtggc tctgcaacga gaacccggtc gccgcgtgcg cgtcgcggaa
     1081 ggcgcgctcg tcgatctcgg tgagcgcccc gtgcggcccg ttgtgctcgg ggtgacacgg
     1141 gccgcacgcc ggcgtctgac catcgccggc caggccgccg tgcaggccgt ccccggcctc
     1201 gagctcggcg gccaccccgg tgtggcagtc gaggcagccg tcggccatgg tgcgcccgct
     1261 ccagggagcc gggtggcagg cgccgcagtc gccgtcgagc tcggcgtggc tggcgacgcc
     1321 gccggcgggt ccggcccggc ccgtgtccgg cgctccggcg ctggtctgag cgttcagccc
     1381 gcccgggctg aacagctccg gtccggcgag gtgcgtcacc gccccggcgc ccaggaagac
     1441 gacgacggcc gccacgatac cggtggccga caggaatccg gggcgcttct tcatgccgtc
     1501 accgcccggt caccgctgca gcgtcgcgta gtagagagcc gcgatgacgt ggatgatcgc
     1561 ggccgcgaag agtgcccacg tcagcggcag gtggacgagg tgccaggtgg cgagggcgcg
     1621 ccgccgcgcc gccgcctcag gcgcgcgcgg cacccgcgtg tagaagtacc gtcccgtcag
     1681 accactggcg accacgacga cggtgagcag catggtcagc ccggcgagcc cgctgaaccg
     1741 gccggccgtg tgcagcagca ccaggtacgg tcccacgagg ccggtgacca tgtggaacct
     1801 gagccaagcc gccgtgctcc cccagcgggc gtcgctgcgc agcttgcgca gcgagtacag
     1861 cgtcgcggtc atcagcatca gcacggaccc ggcgatgccg aacccgtggc cgacgagccc
     1921 ggaggcggcg gggagccgcc ccgcctgcga cgcggcggcc gcgtacagcg ccgtggccgc
     1981 gacgatcgcg gcgagcgccc acaggagctc gcggccgcgg gcccgggcgg ccgcgccgcc
     2041 ggccccggct ccgcggccgg cctctgcctg gccggtgacc ccggtccgcg cgctagacac
     2101 gacacgcccc ccagtcggcg cgcgcgttgc agatgacctc ggccagcggc gtcgcgatca
     2161 cgcgctcggc cgtgctcagg tcgacgcgcg cctcgaccag ctcctgcagc gcgtaggaga
     2221 gggcctggtc gcccatcacc acggcgccgt cgatggtgcg ctctcccagt gcgacacgca
     2281 cgtgcgcctc tccgcgctcc acctccacca cgtccgactc ggtgagctcc gaccacacct
     2341 ggctgtctcc gcgagagagc ccctgcaggt cggcgtcgcc gccgttgccc accgtgccga
     2401 tgatggtggt gtgcaggccg gccagccgcg tgatgttgag cggcctgtcc tcggcgtaga
     2461 cctgccgggg ctccgtcgcc atgttcaggc cggccaccgt ccccttggcg acggccacgt
     2521 tccagagcac gttgagcacg ccgcggccgc tctccgggtc ggcgatctcg gcgatgtcgc
     2581 cggcggcgta gatgtccggg tcgccggcgc gcaggtactc gtcgacgagc acgccgcgcg
     2641 cgcacggcag cccggccgcg cgcgccagct ccaccttggg gcgcacgccg acggccacgg
     2701 cgaccaggtc gcaggggatg gtggcgccgt cctgggtctg gaccccgctc accttgccgc
     2761 cgcggccggc gatgccggcg aggtctgtga acgggtggat ctcgaccccc tcgcggcgca
     2821 gccgccgctc gacgatggtc gactctgagg cggagagcac gttgctccag taacggtcct
     2881 tgcgcatgaa gtagtggacg cgggcgccgc gggcgcgcat cccctccacg atctccagcg
     2941 cggtgatgcc gccaccgacc acgaccgccg cgttcgcctc gcggctgcgg cggatgatgt
     3001 cgcgggcgtc gtccatgttg tcaagtttga caacgccgtc caggccggcg ccggggacgc
     3061 ccgccgggat cgccgtggag cccgtggcga ggagcaggcg gtcgtactcg agccgccggc
     3121 cgctcgccag caccacctgg tgcgcgcccg gctcgatggc ctcggcgcgc tcgccgatcc
     3181 acgtgatccc cagcgcgcgc agctcggccg cgctgaaggg gaacagccgc cgctcgggca
     3241 gctcgtcggt gaggtagtag gccaggccgg ggcgggagta gaagccgtgc gggtcggcgc
     3301 cgacgacgac gatctgagcc tccgcgtcgg cgccgcggat ggcctccgcg gccgcgatgg
     3361 tcgccggccc tccgccgatc agcacgtggc ggcggggcgt ccccggccgg ccggcccggc
     3421 gccccggcgc gatcccgtcg cctgtcgcgc ccagcggctc gaagtgcagc agcccccgcg
     3481 ggcagcgttt ggcgcactcg ccgcaggtca ggcagtggct gtcgccgacc ggctcgccgc
     3541 gccaggcgtg gcggcgcacg tcgagcccca tcgggcagtt gaaggcacac acgccgcact
     3601 gcacgcaccg gcgcagctcg ggcacgaccc acgcgcccgc cagcgtccgg tacttctctc
     3661 tgtcggcggt gaagaacccc acgtccgcat ggtagcgcct gtctcgccca cgtgccgcgc
     3721 aggaagggtt gcaacgcttg tcgccgcccg acctgccggg ccgcggggga aaccactctc
     3781 aggggcccgc gcgagcagcg ctgagggccc atcgacgagg agggaggtcg ccatgacccg
     3841 catcgagcgg agcgtgcaca tcgaggagaa gccggaggtc gtcttccgca tcctcacgga
     3901 cctgaagctc atcccacgct gggcggtgat cgcgcaggac acctacgtgc gaccggagag
     3961 catgctcacg gaggcgcagc agacgttccg ctggaccatc cgcatcgccg gcatcgacct
     4021 ggagaccgac tggaccgtga ccgagttcga cccgccgcgg gcggtgacgt acgcggtcga
     4081 gtcgccgcac gacgggcgtc tggagatgaa gcagcgcgtg ctcgacgcgg gcgacggcgg
     4141 cagccgcgtc gagctcgagg tggactacga cctgccctac ggcttcctcg gtgaggcgct
     4201 cgacaaggtg gtggtggagc accgcaacca ggccgtggtc gagcgctcgc tgcggaatct
     4261 gaaagacctc gtggaggcga tcagggacta cgccgcggcg tgacgtcact gcccgagtcg
     4321 ctcgcggcgg gcgcgcaggt aggcgacgag cagggcgtcg cgttccgcga ccagctcgtc
     4381 gtaggtgcgc gcccccttct cctcggcgag gccggcatcg agggcttcgc cggtctcggg
     4441 cgggaaggtg gtccacgtgg cgaggtcgcg cagcatgccc tccttgacgg cggccaggtg
     4501 ctccacgtgg ccgacgatgc cctcctcgcc gccgccgaga tcgtagagca ggtgcgggcc
     4561 catggcggcc cagcgcaggc cgggcccgta gctcacggcc ttgtccacgt cctcgacggt
     4621 ggccacgccg cgcagcacga ggtccacggc ctcgcgccag agcgcggcgg agagcctgtt
     4681 ggcgaggtag ccgggcaggt cgcgggtgag cttgaccggc tccttgccga ccgcggcgta
     4741 gaaggcggcg gcgcgctcca tcgcctccgg cgaggtcagc gcgccgggcg ccagctccac
     4801 gagcgggacc aggtgcggcg ggttgtacgg atgggcggcg aggcagcgct cggggcgcgc
     4861 cgccgccgtc tggatgtcgc tgatcgagag tcccgacgag ctgctgcaga tcagcgcgtc
     4921 gggaggcgcg gccgcgccga ccgccgcgaa cacctcgcgc ttgagcgcca ggtcctcgcg
     4981 cacgcactcc tgcacgtgag tgacgccggc gaccgcgccc gcgaggtcct cggtcgcggt
     5041 caggagttcc acaccgcgct cggccgcctg catgtcggcg aggccgctcc cggcgaggaa
     5101 ccccgccgcc gcggcggcgc gctccacggc ggcctccagc cgctcgccgt ccacatcctg
     5161 caggcgcact tcgagccccg cggccgcgta gaggccggcc cagcttgctc cggtcaggcc
     5221 ggccccgacc accgccaccg gcccgcgcgg cgcgttcgct gcatccctca tcgcgtccgt
     5281 ccttcctctc gttggcgcga ttctatgcgc cggcgccggg acgggcgcga cgaaggggcg
     5341 gcggggagga cgctgcgtcc gccccgccgc ccctggtcac ccgcggcgcc gggaaggatc
     5401 acccgccggc gccgcgctcc tcacgccgcg cggcgtcgct cgtcgtagcc ttcgtcgaga
     5461 ccttcgtcgg tctcctcgac ggtgccgtag cggaggcgct cctccgggct ccggggctcg
     5521 ctgccgacgg ccgaggcggt cgcctcggcg gacgtctccg cggccgacgt cgcggcggac
     5581 ctctccggca tcggctcctc gacggtcacc cgctcgatcc tgcgcacgcg ctgtggttcg
     5641 cgggcgagct cgcgttcgtt cttgggcgcg ccgtagtaga agtcggtgtc gtcctcgctc
     5701 gggccggcgg tggacctgcc gcgctcctcg gagctcacgt cccggctgcg ggagcgggcc
     5761 agggtggcgg ccagcatcgg gagcgccagc acgccgccga cgccgacgac gatgtacacc
     5821 acgcgctcgc ccaggctgcc gaccgtctgc gtcccggatt gcgtgaacag ccactggacg
     5881 aagttccact cgaacagacc taccagcagc cagttgacgg cgccggcgat ggccagcagc
     5941 aggccgagcc ggccgatcat gtcgtacggg ttcctcatgg tactcaaccc tctcgggtgc
     6001 tcgtggcggc ggtcgttctc cagcgc
//
LOCUS       DASUMX010000347        16364 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1433554, whole genome shotgun sequence.
ACCESSION   DASUMX010000347 DASUMX010000000
VERSION     DASUMX010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 16364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16364
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1433554"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VFZ86_16095"
     CDS             <1..273
                     /locus_tag="VFZ86_16095"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013223801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX5643834.1"
                     /translation="SAAVDIFVGTAAQGRGVGPDAMRTLAAWLVDARGHHRLTVDPAL
                     ENARAIHVYEKLGFRRVGVLRQYERVEDGVWRDALLMELLAGEGGR"
     gene            270..1268
                     /gene="add"
                     /locus_tag="VFZ86_16100"
     CDS             270..1268
                     /gene="add"
                     /locus_tag="VFZ86_16100"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEX5643835.1"
                     /translation="MSATTYPKIELHVHLEATIRPERMLELARRNHVKLPARSVAGLE
                     RFCRFRGFDEFIAVWVKTTGVLCHGRDFRQIVVDYAAELAAQECLYAEPLFSPSEPVL
                     RGVAWQEVFDGYCDGAQEARERYGVELRFTPDITRNFPPEVGEHLAAWAVRYRDRGVA
                     GISLGGSESRFPPAPFARAFAIAREGGLKAAPHAGETAGPASIRSALDDLHADRLRHG
                     VRAVEDAALLAELAERGVVCDVTPTSNLRTGVVRSLDEHPLPVMLAAGVKCSIGSDDP
                     VLMQTSLSADAAVAVRLGHTPRAMYEHGVAGAFCDEPTRARLRAAGEAYDWGLATP"
     gene            1405..2625
                     /locus_tag="VFZ86_16105"
     CDS             1405..2625
                     /locus_tag="VFZ86_16105"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HEX5643836.1"
                     /translation="MVAAVALLFGVAALLLPESSVEVLGVPLVVAGACLGGLRGGLLT
                     ALWAILVITVSFFVLHDVQPDHYAVSVAAYASVGIGLGWAVERVVTQRRQLQDTIAEL
                     QATQRQLWASQKRYRLLFEASNDAVYLHGLDAHGEPSAFAAVNNAACRLFGYTRDELL
                     RLTPRAVDAPATPGQLRRVMERLLHEDAIVYESARKTRDGRQVPVEISSSMTDVDGDL
                     MVLSISRDIAARKKKELRLEQLSLRDELTGLHNRRGFYVLLPEQGKRAKRSGARVVVL
                     YGDIDRFKAINDRLGHRRGDQVLRAVADALRVAFRETDLIARLGGDEFCVVAESPSDP
                     EILVRRLDEAIVAAGGDLGLHIGLSYGTVVTDWRGLDDPDELLTRADMRMYEAKRARK
                     REGETPPGEMAAGA"
     gene            2927..4672
                     /locus_tag="VFZ86_16110"
     CDS             2927..4672
                     /locus_tag="VFZ86_16110"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX5643837.1"
                     /translation="MTPSTTDTTISATPERAPSTGAAPAEGGQKPRRKRGGRRRHGSG
                     GADGAAQTGGAQTQTTGRDTPAAGYAAEAAQAAPAVVPERVERSGDGGESRSRRDERG
                     RDDRGRRDDRGGKGRGDRDRDRGRGRDRQGRGGQGVDESYTERKHIEQPAGPAPEVEI
                     PAFAALGLEGKLLAGVAAMGYSEPTPIQEHAIPRVLAGRDVVGCAQTGTGKTAAFVLP
                     ILQNMSKPMVVDGKKAHPTALVVTPTRELCGQIEEVGETLATYSGRRVVAVFGGVPYD
                     PQAKRVRKGVDLLVATPGRLLDMLRQGDVLLDKVDTLVLDEADRMLDMGFWPDVKRII
                     DAIPSTRQNLFFSATMSKTVLSIIADTLHDPIFIELGGTAQPVDNVEQRVLPVNHEQK
                     VDLLVEYFRHHDPDRTLVFTRTRRRAERLARTLNKAGITCEAIHGDRTQGQRQQALDG
                     FKNGKIAVLVATDVVARGIDVDNITHVINFDIPTNPEDYVHRIGRTARAGASGIAVSL
                     LTAEDVHDLVAIERLIGATLERHDLPDFEYDKRVILESSQVRPRPGKLLWNGGAHRAW
                     NKTGVKRRVPGKRSR"
     gene            4755..4830
                     /locus_tag="VFZ86_16115"
     tRNA            4755..4830
                     /locus_tag="VFZ86_16115"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4788..4790,aa:Val,seq:gac)
     gene            complement(4877..5149)
                     /locus_tag="VFZ86_16120"
     CDS             complement(4877..5149)
                     /locus_tag="VFZ86_16120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643838.1"
                     /translation="MRIRLPRLQRDKRFRLFIADADDAPLDRWFGSLKAALRAFDQLD
                     ERWKRHAWIIEYQEEPRFGGIPVTRDVVHVRHGELTGDAGPDSPGD"
     gene            complement(5169..6119)
                     /gene="trpA"
                     /locus_tag="VFZ86_16125"
     CDS             complement(5169..6119)
                     /gene="trpA"
                     /locus_tag="VFZ86_16125"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEX5643839.1"
                     /translation="MSVRKLPYRPEDHGDHAQPAPDEHGPGPADDAGEPRALPRDLAP
                     RLARAFSAPRAGRAALIPYLTGGHPDTETSERLVATLIDAGADVVELGVPFSDPIADG
                     PVVQQSTHAALMAGVTPDDVLRLAAAHGARAPFVLLTYLNSVLAYGPERFFARAAGAG
                     VEALVIPDLPLDEAAGRSVSLAREAGGLAALAAGAGVTLVPMATPTSTGERLDLVAAA
                     ATSFVYCVAVTGVTGARAEVGAELPALLARLRERTDAPLAVGFGISTPEQAGSVATLA
                     DGVIIGSALIDAVSRAATPDDACAAARTLVSAASTAIAGA"
     gene            complement(6116..7378)
                     /gene="trpB"
                     /locus_tag="VFZ86_16130"
     CDS             complement(6116..7378)
                     /gene="trpB"
                     /locus_tag="VFZ86_16130"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017329990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEX5643840.1"
                     /translation="MTIETRFGPYGGRFVPETLIPALDELTAAYEEARDDAAFQAELA
                     RLLDDYAGRPTPLYHAERLSARCGATVLLKREDLCHTGAHKINNVLGQALLAKRMGKG
                     RIIAETGAGQHGVATATACALLDLECAVYMGAEDVRRQELNVVRMRLLGASVVPVESG
                     SRTLKDAMNEALRDWVTNVRGTFYCIGSVAGPAPYPAMVRDFQAVIGDEAIAQVRERF
                     GHLPDEVVACVGGGSNAMGIFHAFRDLASVRLTGVEAAGEGLDGRHGAPLAKGSPGVL
                     HGSYSYLLQDAWGQVAEAHSISAGLDYPGVGPEHSWLKDSGRAAYAAATDEEALAAFR
                     TLAELEGIIPALESAHAVAYVLRGPSGVEPTAPSPSATGRFTVPDETASFDPAGTRFA
                     EGDLVVVNLSGRGDKDVYEAGAQLGVLP"
     gene            complement(7375..8100)
                     /locus_tag="VFZ86_16135"
     CDS             complement(7375..8100)
                     /locus_tag="VFZ86_16135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEX5643841.1"
                     /translation="MAGVTRVKVCGLTRAEDVRAAADLGAWALGFVLTESPRRVDVAR
                     AGQLTKVARDAGASAARTPARDASSAHVPPLTVGVVTTESPAWIAAALEASRLDAVQL
                     SAGADGPAVAEVLAAARREPRPLVIAAADTPDASLADFTLLDARAPGAYGGTGLTLDW
                     RALDDELGTPRERLVLAGGLTPANAADAVRALRPAAVDVSSGVESVPGVKDAELLHAF
                     FAAVEHADKDAAEPATATRRTSA"
     gene            complement(8088..8966)
                     /locus_tag="VFZ86_16140"
     CDS             complement(8088..8966)
                     /locus_tag="VFZ86_16140"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEX5643842.1"
                     /translation="MSAERQPLFPGARPATAYEQPAPGGFLAEMIAGSRRRVAVARIA
                     GRLQRPATAGEPGRLRDALSRPSWTPGAPSPQLGVIAEVKRRSPSKGDIAPDLDAVAQ
                     ARAYAAAGADAISVLTEPTRFGGSLDDLRAVAAAVDLPVLRKDFIVDRGQIWEAADAG
                     AAAVLLIVGILSDDRLHELLIEALDCGLDPLVEVHDLEETRRACRAGCTLVGINNRDL
                     VTLEVDLETTEYLAPALGPCMFPVSESGISTPVDACRAALSGARALLVGETLVRTPAE
                     DLPGLIASLKDPGWPA"
     gene            complement(8963..10009)
                     /gene="trpD"
                     /locus_tag="VFZ86_16145"
     CDS             complement(8963..10009)
                     /gene="trpD"
                     /locus_tag="VFZ86_16145"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEX5643843.1"
                     /translation="MPNDVLSAALAELTAGRDLTEESARGVLLEIMGGRAGEAQTAAF
                     LSALRVKGETAAEIVGMARAMSELAEKVAVDADVILDTCGTGGDAAGTFNISTAAALV
                     AAAAGVTVAKHGNRSATSKCGSADVLEALGVAIDLPPDRVSRCIAACGIGFMFAPRHH
                     LAMKHVALVRRELGMATTFNLIGPLTNPAGARHQLIGVADARYVDRIAQAVRMMGNAR
                     NLIVHSDDGLDEISTTCATAIVEVFAGQGYDERYDVTPEQFGMERARPEDLAGGDAAA
                     NAALLRAVVDGEPGPRLDIVVLNAGAALYIAEAAGSIAEGVATARAAVASGAARAKLD
                     ALVATSRRLARESS"
     gene            complement(9999..10673)
                     /locus_tag="VFZ86_16150"
     CDS             complement(9999..10673)
                     /locus_tag="VFZ86_16150"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010795966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEX5643844.1"
                     /translation="MTTTAPRPGDGARVFMIDNYDSFTYNLVQYLGELGADITVKRND
                     QVTVAEIAAAAPTHLVVSPGPCTPNEAGISMAAIAHFGAARLPVLGVCLGHQAIGQVY
                     GGVVRRAGAPVHGKTDQIHHEGAGVFAGLPDPFTATRYHSLVVDEALPDCLALTAWNA
                     EGIVMGVGHRELPVHGVQFHPESVLTEVGLDLLATFLRTTGRESGAAAAGATASAAAE
                     EARDAE"
     gene            complement(10673..12229)
                     /gene="trpE"
                     /locus_tag="VFZ86_16155"
     CDS             complement(10673..12229)
                     /gene="trpE"
                     /locus_tag="VFZ86_16155"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEX5643845.1"
                     /translation="MAKDIRVTPELVEARRLALAHNVLPVTHSFVSDLETPVSAFLKL
                     GAPRRAFLLESAEQGQHLGRFSFLGVGARAVVTYAGGRLTVRENGDRLSVEHDDPFTA
                     VADLMRSYRPPASGAAASLPPLAGGLVGYFGYDLVRRLEPLPDAPPDDLGLPEMAFLL
                     ADTVLVFDHLKHAITIVANAFVDGRDVDGPYARACERIADVRARLAAPLPAGAAGNLV
                     DAAAVDADAGAAGRAAAGGPVTSSMTRDQYEAGVERIRDYIYAGDAFQVVLSQRFSTP
                     VDVSPFSIYRGIRAVNPSPYLYYIAFDDFALCGSSPEPLVTVQGERVETRPIAGTRRR
                     GADPAEDRRLIEDLRADEKERAEHVMLVDLGRNDLGRVCVPGTVTVDEFMEMELYSHV
                     IHMVSSVRGTLAAGSDPTDALKAAFPAGTVSGAPKVRAMQIVDELEAVRRGPYAGAIG
                     YLSYGGDLDTCICIRTVIVKDGVAHVQAGAGIVADSVPEKEYAETQNKAAALLRAIDL
                     AHEQFGDGGT"
     gene            complement(12372..13034)
                     /locus_tag="VFZ86_16160"
     CDS             complement(12372..13034)
                     /locus_tag="VFZ86_16160"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HEX5643846.1"
                     /translation="MTEFEGLDPSSLAARIAALDEQGALDAVQGRIAAGDDPLAIIEE
                     CQVGMRWVGEHYQSGKYFISGLIMAGEIFREAMVALGPLLPPETADAGQGSVLIATVR
                     GDIHDLGKNIVTMMLRSYGIVVHDLGVDVSPDEVVRKAVELQPDIVGLSGLLTVATDG
                     MKATVEAVRAEPRLGRRLPVIIGGGIVDEQMRAWTGADLWASDASRGVRLIREAIAAA
                     RD"
     gene            complement(13034..13849)
                     /locus_tag="VFZ86_16165"
     CDS             complement(13034..13849)
                     /locus_tag="VFZ86_16165"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX5643847.1"
                     /translation="MTQSAEGSRRMATIPPGRGIDRSSYEPAYIQLVNILRGAIASGQ
                     YQAGDQLPTESELCAAYDVSPMTVRRAIGMLLDQGAVSTTRGRGTFVQPLRLATATFD
                     LSEFHDILAEPGVTAKVVEARVLPAGPRAAAHLGVADGTHVVSIRRILQRGDEPLIYH
                     RESLIYDPTRPTIESELDVTALRDMFEGGVGSGPKRGELVLHASALSEDEASRLALAP
                     GVAAWVLEHIFYGYDEKPISWGCFICRGDLFSFRASVGAPAPGGDTGARGNRR"
     gene            13966..14601
                     /locus_tag="VFZ86_16170"
     CDS             13966..14601
                     /locus_tag="VFZ86_16170"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HEX5643848.1"
                     /translation="MSTELTDAIVGMREDEAMRLVAAMLDAGADPADVLEEAKAAMTV
                     LGDKFEREEVFIPELIMGGEIMKGIADELKPRIKGEAAAEHRGTVVLGTVAGDIHDIG
                     KDVVVLMLDVNGYDVHDLGIDVPIEKFVAAVRELKPQVVGLSGLLTLAFDAMKATVAG
                     IADAGLRDEVRIMIGGSPVDEQICAYTGADGWGRDAAAALRMAAEWTGGAA"
     gene            14598..15854
                     /locus_tag="VFZ86_16175"
     CDS             14598..15854
                     /locus_tag="VFZ86_16175"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HEX5643849.1"
                     /translation="MTPTPEELYAQREQRFNDAVALKKTDRVPVMPLYVHNFPTLIKG
                     ISNKDAGYDHETRWQSTKEATLRFGWNFAPTNDVLASDFFAAYGNKQIMWAGDEGGLA
                     DDAPFQWVEDEYVKADELAEFIADPNGFTFNKILPRMGSKLEGLGQIPLPPLYWFSNS
                     YYLQVLGNMLGTPPLKAALQAMLDMAEAQEKTNAANGKYLQEMKDLGYPPTWGAALMP
                     AFDVVSDTFRSLRGSTLDMYRQPDTLLEAIAVMQEATLGLLHAAFAVTGIPRQFIPMH
                     KGAGGFMSNEQFAKFYWPTFRALIDECIAAGITPMPLFEGDYTARLEFLAELPPGKVA
                     AHFDRMDRKKFKEACGDVLCFWGNVPSSLLVTGTPQQVKDDVKELIDLFGETGLILDG
                     PGGIPDQAKPENVEALAEAVEEYGTL"
     gene            16015..>16364
                     /locus_tag="VFZ86_16180"
     CDS             16015..>16364
                     /locus_tag="VFZ86_16180"
                     /inference="COORDINATES: protein motif:HMM:NF014646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing protein"
                     /protein_id="HEX5643850.1"
                     /translation="MTQELTQAIVDLREDDALAMVDDLLASGADAVGILADCKAAMDV
                     IGEKFAGGEAFIPELIMAGEIMTGVSAKLKPHLVGSAPEEKLGVIVIGTVQGDIHDIG
                     KDIVATMLDIAGFDV"
BASE COUNT         2312 a         5814 c         6051 g         2187 t
ORIGIN      
        1 tcggcggccg tggacatctt cgtcggcacg gccgcccagg gccgcggggt cggtccggac
       61 gccatgcgca cgctcgcggc ttggctcgtg gacgcgcgcg ggcaccaccg cctcacggtc
      121 gacccggcgc tcgagaacgc gcgcgcgatc cacgtctacg agaagctggg cttccggcgc
      181 gtgggcgtgc tgcggcagta cgagcgcgtc gaggacgggg tctggcgcga cgcgctcctc
      241 atggagctgc tggcggggga ggggggccgt tgagcgccac gacgtacccc aagatcgagc
      301 tgcacgtgca cctcgaggcg accatccggc ccgagcgcat gctggagctg gcgcggcgca
      361 accacgtcaa gctcccggcg cggtcggtgg cgggactgga gcgcttctgc cgcttccgcg
      421 gcttcgacga gttcatcgcc gtgtgggtca agaccaccgg tgtgctgtgt cacggccggg
      481 acttccgcca gatcgtcgtc gactacgccg ccgagctggc ggcgcaggag tgcctgtacg
      541 ccgagccgct gttctcgccg tccgagccgg tgctgcgcgg ggtcgcgtgg caggaggtgt
      601 tcgacggcta ctgcgacggg gcccaggagg cgcgtgagcg ctacggcgtg gagctccgct
      661 tcacgcccga catcacgcgc aacttcccgc cggaggtcgg cgagcacctc gccgcgtggg
      721 cggtgcgcta ccgcgaccgg ggcgtggccg gcatcagcct cggcgggagc gagagccggt
      781 tcccgccggc gccgttcgcg cgcgccttcg cgatcgcccg cgagggcggg ctcaaggcgg
      841 cgccgcacgc cggcgagacg gccggcccgg cgtccatccg ctccgccctg gacgacctgc
      901 acgccgaccg cctgcggcac ggcgtgcgcg ccgtggagga cgccgcgctc ctggcggagc
      961 tggcggagcg cggcgtcgtc tgcgacgtca cgcccacgag caacctgcgc acgggcgtgg
     1021 tgcgctcgct ggacgagcac ccgctgccgg tgatgctcgc cgccggcgtc aagtgctcca
     1081 tcgggagcga cgatcccgtc ctcatgcaga cgtcgctctc ggcggacgcc gccgtcgccg
     1141 tccgcctcgg gcacacgccg cgggcgatgt acgagcacgg cgtggcgggc gcgttctgcg
     1201 acgagccgac gcgcgcccgc ctcagggcgg ccggcgaggc ctacgactgg ggcctcgcca
     1261 ccccgtagcc gcgcgccgcg atctgcgccg ccctcaacgc tcaaggcgcc cgtcttgctg
     1321 ccgacaggga ggacgaaggg cacgacgacc ttgtcgcccc cgccgacgaa ggaggccccc
     1381 ctgaacgccg tacgcgcgaa ccgcgtggtc gccgccgtgg ccttgctgtt cggcgtcgcc
     1441 gcgctgctcc ttcccgagtc ctccgtggag gtcctcggcg tgcctctcgt cgtggcgggc
     1501 gcgtgtctcg gcgggctgcg cggcgggctg ctgacggcgc tctgggcgat cctggtgatc
     1561 accgtctcct tcttcgtgct ccacgacgta cagccggacc actacgcggt gagcgtggcc
     1621 gcctacgcgt cggtgggcat cggcctgggc tgggcggtcg agcgcgtcgt cacgcagcgg
     1681 cggcagttgc aggacaccat cgccgagctg caggccaccc agcgccagct ctgggccagc
     1741 cagaagcgct accgcctcct cttcgaagcg agcaacgacg ccgtctacct gcacggcctg
     1801 gacgcgcacg gcgagccgtc ggccttcgca gccgtcaaca acgcggcctg caggttgttc
     1861 gggtacaccc gagacgaact gctgagactg acgccgcgcg ccgtcgacgc gccggccacc
     1921 ccggggcagc tccggcgggt gatggagcgg ctcctgcacg aggacgccat cgtgtacgag
     1981 agcgcgcgca agacgcgcga cggccggcag gtgcccgtgg agatcagctc cagcatgacc
     2041 gacgtcgacg gcgatctcat ggtcctgtcg atctcgcgcg acatcgccgc ccggaagaag
     2101 aaggagctcc gcctggagca gctctcgctt cgcgacgagc tcaccggtct gcacaaccgc
     2161 cgcggcttct acgtgctgct gcccgaacag ggcaagcgcg cgaagcgctc cggcgcgcgc
     2221 gtcgtggtgc tgtacgggga catcgaccgc ttcaaggcca tcaacgaccg gctggggcac
     2281 agacgcgggg accaggtgtt gcgggcggtc gccgacgcgc tgcgtgtcgc cttccgcgag
     2341 acggacctga tcgcccgcct cggcggcgac gagttctgcg tggtcgccga gtcgccctcc
     2401 gatccggaga tcctcgtccg ccgcctcgac gaggcgatcg tcgcggccgg aggagacctc
     2461 ggcctgcaca tcgggctcag ctacggcacg gtggtcaccg actggcgcgg gctggacgat
     2521 cccgacgagc tgctcacccg cgccgacatg cggatgtacg aggccaagcg cgccaggaag
     2581 cgcgaggggg agacgccccc gggggagatg gcggccggcg cgtgagcgtt gccgcttgag
     2641 ggtacaatcg agacctgata aggttggatt cagaagacgg gagccggttt cgtcccaggc
     2701 gccacgcgcc gggccttcct cccgcagcag acccccagac attgtgacca ccgggcagtg
     2761 ttctgaaagg aaaggaacac cagtccgtac cgagtctggc cgccccgagg gcagcccccc
     2821 acaagacgaa ctcccgcgca cgccgctgat tcccggggag cattcacctg ctcccgtgga
     2881 gagcgaggcg tcgccgcgta cggagccgtc gaggaagaga gcatctgtga cccccagcac
     2941 caccgacacg accatctccg ccacccccga gcgcgccccg tccacgggcg ccgcgccggc
     3001 cgagggcgga cagaagcccc gccgcaagcg cggcggccgg cgccgccacg ggagcggcgg
     3061 cgccgacggc gccgcccaga ccggcggcgc gcagacgcag accaccggcc gggacacacc
     3121 ggccgccggc tacgcggcgg aggcggctca ggccgcgccg gccgtcgtgc ccgagcgcgt
     3181 cgagcgctcc ggcgacggcg gcgagagccg cagccgccgt gacgaacgcg gccgtgacga
     3241 ccgcggccgg cgagacgacc gtggcggcaa gggccgcggc gaccgcgacc gtgaccgcgg
     3301 ccgcggcaga gaccgccagg gccgcggcgg ccagggcgtc gacgagagct acaccgagcg
     3361 caagcacatc gaacagccgg ccggacctgc ccctgaggtc gagatccccg cgttcgcggc
     3421 gctcgggctc gagggcaagc tgctggccgg cgtcgccgcc atgggctact cggagccgac
     3481 gccgatccag gagcacgcca tcccgcgcgt gctcgccggc cgcgacgtcg tgggctgcgc
     3541 ccagaccggc accggcaaga cggccgcctt cgtgctgccc atccttcaga acatgagcaa
     3601 gccgatggtc gtcgacggca agaaggcgca tcccaccgcc ctcgtcgtca ccccgacccg
     3661 cgagctctgc ggccagatcg aggaggtcgg cgagacgctg gccacctaca gcggccgtcg
     3721 cgtcgtcgcc gtcttcggcg gcgtgccgta cgaccctcag gccaagcggg tccgcaaggg
     3781 cgtcgacctc ctggtcgcga cccccggccg cctgctcgac atgctccgcc agggtgacgt
     3841 cctgctcgac aaggtcgaca cgctcgtcct cgacgaggcc gaccgcatgc tcgacatggg
     3901 cttctggccg gacgtcaagc gcatcatcga cgccatccct tccacgcgcc agaacctctt
     3961 cttctcggcg accatgagca agaccgtgct cagcatcatc gccgacacgc tgcacgaccc
     4021 catcttcatc gagctcggcg gcaccgctca gccggtcgac aacgtcgagc agagggtgct
     4081 gccggtcaac cacgagcaga aggtcgacct cctcgtggag tacttccgtc accacgatcc
     4141 cgatcgcacc ctggtgttca cgcgcacgcg ccgtcgggcg gagcgcctcg cccgcaccct
     4201 gaacaaggcg ggcatcacct gcgaggccat ccacggcgac cgtacccagg gacagcgcca
     4261 gcaggcgctc gacggcttca agaacggcaa gatcgccgtg ctcgtcgcga ccgacgtggt
     4321 cgcccgcggt atcgacgtcg acaacatcac gcacgtgatc aacttcgaca tcccgaccaa
     4381 ccccgaggac tacgtgcatc gcatcggccg cacggcccgg gcgggcgcca gcggcatcgc
     4441 cgtgagcctg ctcaccgccg aggacgtgca cgacctcgtc gccatcgagc gactcatcgg
     4501 ggcgacgctc gagcgccacg acctgccgga cttcgagtac gacaagcgcg tcatcctcga
     4561 gagcagccag gtgcggccgc gtcccggcaa gcttctctgg aacggtggcg cgcaccgcgc
     4621 ctggaacaag accggcgtca agcgccgcgt gccgggcaag cgctcgcgct gacgcttctt
     4681 cagaggtcgc ggcgcggcgt cacagcctcc ggctgacacc gcgccgcgac cttgctatac
     4741 tcccgttcct gcgtgggcga ctagctcagt tgggagagcg cctgctcgac aagcaggagg
     4801 tcgttggttc aattccaacg tcgcccacca tctcctcact tctccgcctt gcggccgcgc
     4861 cgcgccggga atccgttcag tcgcccgggg aatcgggtcc cgcgtcgccg gtgagttcgc
     4921 cgtgccgcac gtggaccacg tcgcgggtca cggggatgcc gccgaacctc ggctcctcct
     4981 ggtactcgat gatccaggcg tggcgcttcc agcgctcgtc cagctggtcg aaggcgcgca
     5041 gagccgcctt cagcgagccg aaccagcggt cgagaggggc gtcgtctgcg tcggcgatga
     5101 acagacggaa gcgcttgtct ctctgcagac gcggaaggcg gatcctcatg cagcgccccg
     5161 gggtgtggtc aggcgccggc gatcgccgtg ctcgccgcgc tcacgagcgt gcgtgcggcc
     5221 gcgcaggcgt cgtcgggtgt ggccgcgcgc gagacggcgt cgatgagcgc gctgccgatg
     5281 atcacgccgt cggccagcgt cgccacgctg ccggcctgct ccggcgtgga gatgccgaac
     5341 cccaccgcga gcggcgcgtc cgtgcgctcg cgcaggcgcg ccaggagcgc gggcagctcg
     5401 gcgccgacct cggcgcgggc gccggtcacg ccggtcacgg cgacgcagta gacgaacgag
     5461 gtggccgcag cggcgacgag gtcaaggcgc tcgccggtgc tcgtcggcgt cgccatcggc
     5521 acgagcgtga cgccggcgcc ggcggcgagc gccgccagcc cgccggcctc ccttgcgagc
     5581 gagacgctcc ggccggcggc ctcgtcgagc ggcaggtcgg ggatcaccag cgcctccacg
     5641 ccggcgccgg ccgcgcgcgc gaagaaccgc tccgggccgt aggccagcac cgagttgagg
     5701 taggtgagca gcacgaaggg cgcccgggcg ccgtgcgcgg cggcgagcct cagcacgtcg
     5761 tcgggcgtca cgccggccat gagcgccgcg tgcgtggact gctgcacgac gggtccgtcg
     5821 gcgatcgggt ccgagaacgg gacgccgagc tcgacgacgt cggcgcccgc gtcgatcagg
     5881 gtggcgacga ggcgctcgct cgtctccgtg tccgggtggc cgccggtgag gtaggggatg
     5941 agcgccgcgc gccctgcgcg gggcgcggag aacgcgcgcg ccagacgcgg cgcgaggtct
     6001 cgcggcagcg cccgcggctc gcccgcgtcg tcggcagggc cgggcccgtg ctcatcgggc
     6061 gcgggctgcg cgtggtctcc gtggtcctcc ggccggtacg gcagcttgcg cacgctcatg
     6121 gcagcacccc cagctgggct ccggcctcgt agacgtcctt gtcgccccgc cccgagaggt
     6181 tgaccacgac gaggtctccc tcggcgaagc gcgtgcctgc cgggtcgaag ctggcggtct
     6241 cgtcggggac ggtgaagcgc cccgtggcgg agggagaggg ggcggtcggt tcgacgcccg
     6301 acggcccgcg cagcacgtag gccacggcgt gggcgctctc gagcgccggg atgatgccct
     6361 cgagctcggc gagcgtgcgg aaggcggcga gcgcctcctc gtcggtcgcc gccgcgtatg
     6421 cggcgcggcc cgagtccttg agccaggagt gctcgggccc gacgccgggg tagtccaggc
     6481 cggcggagat cgagtgcgcc tccgcgacct gaccccaggc gtcctgcagc aggtagctgt
     6541 aggagccgtg caggacgccc ggcgagccct tggccagcgg ggcgccgtgg cggccgtcaa
     6601 ggccctcgcc ggccgcctcg acgccggtca ggcgcaccga ggcgaggtcg cggaaggcgt
     6661 ggaagatgcc catggcgttg gagcctccgc ccacgcaggc gaccacctcg tcggggaggt
     6721 ggccgaagcg ctcgcgcacc tgcgcgatgg cctcgtcgcc gatgaccgcc tggaagtcgc
     6781 ggaccatggc cgggtagggc gccgggccgg cgacggagcc gatgcagtag aaggtgccgc
     6841 gcacgttggt cacccagtcg cgcagcgcct cgttcatggc gtccttgagc gtgcgcgacc
     6901 cgctctcgac ggggacgacg gaggcgccga gcaggcgcat gcgcaccacg ttgagctcct
     6961 ggcggcgcac gtcctcggcg cccatgtaga cggcgcactc gaggtcgagc agggcgcagg
     7021 cggtcgccgt ggcgacgccg tgctgcccgg ccccggtctc ggcgatgatg cggcccttgc
     7081 ccatgcgctt ggcgaggagc gcctggccga gcacgttgtt gatcttgtgc gcgccggtgt
     7141 ggcagaggtc ctcgcgcttg agcaggacgg tcgccccgca gcgcgcgctc agccgctccg
     7201 cgtggtagag cggcgtagga cgccccgcgt agtcgtcgag gaggcgcgcg agctcggcct
     7261 ggaaggccgc gtcgtcgcgc gcctcctcgt aggcagccgt cagctcgtcg agcgccggga
     7321 tgagcgtctc cgggacgaac cgtcccccgt aggggccgaa gcgcgtctcg atggtcatgc
     7381 gctggtcctc ctcgtggccg tggccggctc ggccgcgtcc ttgtcggcat gttccaccgc
     7441 ggcgaagaac gcatgcagaa gctccgcgtc tttgaccccg gggacggact cgacgccgct
     7501 ggagacgtcc acggccgccg gccgcagcgc gcgcaccgcg tcggccgcgt tggccggggt
     7561 gaggccgccg gccaggacga ggcgttcgcg cggcgtcccg agctcgtcgt cgagcgcgcg
     7621 ccaatccagg gtgagcccgg taccgccgta ggcgccgggg gcgcgcgcgt cgagcagcgt
     7681 gaagtcggcg aggctcgcgt ccggggtgtc ggcggcggcg atgacgagcg ggcgcggctc
     7741 cctgcgcgcc gccgccagga cctcggccac cgccgggccg tcggcgcctg ccgacagctg
     7801 cacggcgtcc agccgcgacg cttcgagggc ggcggcgatc cacgccggcg actccgtggt
     7861 gacgacgccc acggtgagcg gcggcacgtg cgcggaggag gcgtcccgcg caggggtccg
     7921 ggcggcggac gctccggcgt ctcgcgccac cttcgtcagc tgccccgccc gggcgacgtc
     7981 cacccgccga gggctctccg tgagcacgaa cccgagcgcc cacgcgccga ggtccgccgc
     8041 cgcgcggacg tcctcggcgc gcgtgaggcc gcagaccttg acccgggtca cgccggccat
     8101 cccgggtcct tgaggctggc gatgagcccc ggcaggtctt ctgcgggcgt gcgcaccagg
     8161 gtctcgccga cgaggagcgc cctcgcgccg gagagcgccg cgcggcaggc gtccaccggc
     8221 gtggagatgc cgctctcgct caccgggaac atgcaggggc cgagcgcggg cgcgaggtac
     8281 tcggtggtct ccaggtcgac ctcgagggtg acgaggtcgc ggttgttgat gccgaccagc
     8341 gtgcagccgg cgcggcaggc gcgccgcgtc tcctccaggt cgtggacctc caccaaggga
     8401 tcgaggccgc agtccagagc ctcgatgagc agctcgtgca ggcggtcgtc gctgaggatg
     8461 ccgacgatga gcaggaccgc ggccgccccg gcgtcggccg cctcccagat ctgtccacgg
     8521 tcgacgatga agtccttgcg cagcaccggc aggtcgaccg cggccgccac ggcgcgcagg
     8581 tcgtcgagcg agccgccgaa gcgcgtgggc tcggtgagca cggagatggc gtcggcgccg
     8641 gccgccgcgt aggcgcgcgc ctgggcgacc gcgtcgaggt cgggggcgat gtcgcctttg
     8701 ctcggcgagc gtcgcttgac ctcggcgatc acgccgagct gaggactggg cgctcccggg
     8761 gtccagctcg gccgggacag cgcgtcgcgc agccggccgg gctcgccggc cgtcgccggc
     8821 cgctggagac ggcccgcgat gcgcgccacg gcgacgcgcc ggcgcgagcc ggcgatcatc
     8881 tcggcgagga acccgccggg ggcgggctgc tcgtaggcgg tcgccgggcg ggcgccgggg
     8941 aacaggggct ggcgctctgc gctcacgagc tctcccgggc cagccggcga ctggtcgcga
     9001 cgagggcgtc cagcttggcg cgggcggcgc cggaggcgac ggccgcccgc gcggtcgcca
     9061 cgccctcggc gatgctgccg gcggcctcgg cgatgtagag cgccgcgccg gcgttgagca
     9121 cgacgatgtc gaggcgcggc cccggctcgc cgtccacgac ggcgcgcagc agggccgcgt
     9181 tcgccgcggc gtcgccgccg gcgaggtcct ccgggcgcgc gcgctccatg ccgaactgct
     9241 ccggcgtcac gtcgtaccgc tcgtcgtagc cctggccggc gaagacctcg acgatcgcgg
     9301 tggcgcaggt ggtggagatc tcgtccagcc cgtcgtcgct gtggacgatg aggttgcgcg
     9361 cgttgcccat catgcgcacc gcctgcgcga tgcggtcgac gtagcgggcg tcggccacgc
     9421 cgatgagctg gtggcgggcg ccggcggggt tggtcagcgg cccgatgagg ttgaacgtgg
     9481 tggccatgcc gagctcgcgg cgcacgagcg cgacgtgctt catcgccagg tgatgacggg
     9541 gcgcgaacat gaaaccgatg ccacaggcgg cgatgcagcg gctcacccga tcgggaggca
     9601 ggtcgatggc gacgccgagc gcctcgagca cgtcggcgct cccgcacttg ctcgtggccg
     9661 agcggttgcc gtgcttggcc acggtgacgc cggcggccgc ggccacgagc gccgccgccg
     9721 tcgagatgtt gaaggtgccg gcggcgtcgc cgccggtgcc gcaggtgtcg aggatgacgt
     9781 ccgcgtccac cgcgaccttc tccgcgagct cgctcatggc ccgggccatg ccgacgatct
     9841 cggcggcggt ctcgcccttg acgcgcaggg cgctgaggaa ggcggccgtc tgggcctcgc
     9901 cggcccggcc gcccatgatc tcgagcagca cgccgcgggc gctctcttcc gtgaggtcgc
     9961 ggccggcggt gagctcggcg agcgccgcgc tcagcacgtc attcggcatc gcgggcctcc
    10021 tcggccgcgg cgctcgcggt cgcccccgcg gccgcggccc cgctctcacg gccggtcgtg
    10081 cgcaggaacg tcgccagcag gtccagccct acctcagtga ggacgctctc ggggtggaac
    10141 tgcacgccgt gcacgggcag ctcccggtgc ccgacgccca tcacgatgcc ctccgcgttc
    10201 cacgccgtga gcgccaggca gtccggcagc gcctcgtcca cgaccagtga gtggtagcgc
    10261 gtcgcggtga agggatcggg caggccggcg aacacgccgg cgccctcgtg gtggatctgg
    10321 tccgtcttgc cgtgtacggg cgcgcccgcg cgccgcacca cgccgccgta cacctggccg
    10381 atggcctggt ggccgaggca gacgccgagc accggcaggc gcgcggcgcc gaagtgggcg
    10441 atggccgcca tgctgatccc cgcctcgttg ggcgtgcagg gaccagggga gacgacgagg
    10501 tgcgtcggcg ccgcggcggc gatctccgcg acggtcacct ggtcgttgcg cttgacggtg
    10561 atgtcggcgc cgagctcacc gaggtactgc accaggttgt aggtgaacga gtcgtagttg
    10621 tcgatcatga agaccctggc gccgtcgccg ggccgggggg cggtggtcgt catcacgtgc
    10681 ctccgtcgcc gaactgctcg tgggcgaggt cgatggcgcg cagcagcgct gcggccttgt
    10741 tctgcgtctc agcgtactcc ttctcgggca cggagtcggc gacgatgccg gcgccggcct
    10801 gcacgtgcgc cacgccgtcc ttgacgatca cggtgcggat gcagatgcag gtgtcgaggt
    10861 cgccgccgta gctcagatag ccgatggcgc ccgcgtacgg gccacgccgc acggcctcga
    10921 gctcgtcgac gatctgcatg gcgcggacct tgggcgcgcc gctgaccgtg ccggcgggga
    10981 aggcggcctt gagcgcgtcc gtcgggtcgc tgccggcggc cagcgtcccg cgtaccgacg
    11041 agaccatgtg gatgacgtgg gagtagagct ccatctccat gaactcgtcg accgtgacgg
    11101 tcccggggac gcagacgcgg ccgaggtcgt tgcggccgag gtcgacgagc atgacgtgct
    11161 cggcgcgctc cttctcgtcg gcgcgcagat cctcgatgag gcgccggtcc tcggcggggt
    11221 ccgcgccccg ccggcgagtg ccggcgatcg gccgcgtctc gacgcgctcg ccctgcacgg
    11281 tgaccagcgg ctcggggctg gagccgcaga gggcgaagtc gtcgaaggcg atgtagtaga
    11341 ggtacgggct cgggttgacc gcccggatgc cgcggtagat ggagaacggc gagacgtcca
    11401 cgggcgtgct gaagcgctgg ctgagcacga cctggaaggc gtcgccggcg tagatgtagt
    11461 cgcggatgcg ctccacgccg gcctcgtact ggtcgcgtgt catggacgag gtgacggggc
    11521 cgcccgcggc cgcccgccct gccgcgccgg cgtccgcgtc cacggcggcg gcgtcgacga
    11581 ggttgcccgc cgcgccggcg gggagcggcg ccgcgagcct ggcgcgcacg tcggcgatgc
    11641 gctcgcaggc acgcgcgtag gggccgtcga cgtcgcggcc gtcgacgaag gcgttggcga
    11701 cgatcgtgat ggcgtgcttg aggtggtcga acacgagcac cgtgtcggcc agcaggaacg
    11761 ccatctcggg gaggccgagg tcgtccggcg gcgcgtccgg cagcggctcc aggcgccgca
    11821 cgaggtcgta gccgaagtag ccgacgaggc cgccggcgag cggcggcagg gacgccgccg
    11881 cgccgctcgc cggcggccgg tagctgcgca tgaggtcggc gaccgcggtg aacgggtcgt
    11941 cgtgctcgac gctcaggcgg tcgccgttct cccgcacggt cagccgcccg ccggcatagg
    12001 tcacaacggc gcgggcgccg acgcccagga acgagaaccg ccccaggtgc tgaccctgct
    12061 cggcgctctc gagaaggaag gcgcggcggg gcgcgccgag cttgaggaac gccgagacgg
    12121 gggtctcgag gtcggagacg aacgagtggg tgacgggcag gacgttgtgg gccagtgcga
    12181 ggcggcgcgc ctcgaccagc tcaggtgtta ctcgtatgtc cttcgccacg ggccaatcat
    12241 gcgacacctc cggtgagcac cgggcccttc atcgcgtgcc tgccgtcccg gcaggcggtc
    12301 tgggaaccgt agggcaggag acacggcact gtcaagccgc cgcggcgccg ggcggcggcg
    12361 ccggcggacg gtcagtcccg ggctgccgcg atcgcctcgc ggatgagccg caccccgcgc
    12421 gacgcgtcgc tggcccagag gtcggcgccg gtccaggcgc gcatctgctc gtcgacgatg
    12481 ccgccgccga tgatgaccgg caggcggcgg ccgagccggg gctcggcgcg caccgcctcg
    12541 accgtggcct tcatgccgtc ggtggcgacg gtgagcaggc cggagagacc cacgatgtcc
    12601 ggctgcaact cgaccgcctt gcgcacgacc tcgtcgggcg agacgtcgac gccgagatcg
    12661 tggaccacga tcccgtagga acgcagcatc atggtgacga tgttcttgcc caggtcgtgg
    12721 atgtcgccgc ggaccgtggc gatgagcacg ctgccctggc cggcgtccgc ggtctccggc
    12781 ggcagcagcg ggccgagggc gaccatggcc tcgcggaaga tctcgccggc catgatgagg
    12841 ccggagatga agtacttgcc cgactggtag tgctccccga cccagcgcat gccgacctgg
    12901 cactcctcga tgatcgccag agggtcgtcg ccggcggcga tgcgcccctg cacggcgtcg
    12961 agcgcgccct gctcgtccag cgccgcgatg cgggcggcga gcgatgacgg gtcgaggccc
    13021 tcgaactcgg tcatcatcgg cggttccccc tcgcgccggt gtcgccgccg ggcgccggcg
    13081 cgccaacgct cgcgcggaac gagaagaggt cgccgcggca gatgaagcac ccccagctga
    13141 tcggcttctc gtcgtagccg tagaagatgt gctcgagcac ccaggccgcg acccccgggg
    13201 ccagcgcgag ccggcttgcc tcgtcctcgg agagcgcgga ggcgtgcagg acgagctccc
    13261 cgcgcttggg gccgctgccc acgccgccct cgaacatgtc gcgcagggcg gtgacgtcga
    13321 gctcggactc gatcgtcggc cgggtggggt cgtagatcag cgactcgcgg tggtagatca
    13381 gcggctcgtc gccgcgctgc aggatgcggc ggatggagac gacgtgcgtg ccgtccgcca
    13441 caccgaggtg ggcggcggcg cgcggaccgg cgggcagcac gcgcgcctcg accaccttcg
    13501 cggtcacgcc cggctcggcc aggatgtcgt ggaactcgct gaggtcgaac gttgccgtgg
    13561 cgaggcggag cggctggacg aaggtgccgc ggccgcgcgt cgtgctgacg gcgccctggt
    13621 ccagcagcat cccgatggca cggcgcacgg tcatggggct gacgtcgtag gcggcgcaca
    13681 gctcggactc ggtggggagc tggtcgcccg cctggtactg gccgctggcg atggcgccgc
    13741 gcaggatgtt cacgagctgg atataggccg gctcgtacga ggaacgatcg atgccgcggc
    13801 cgggtgggat cgtggccatg cgcctgctgc cctctgccga ctgtgtcatg cgccgcatca
    13861 tatcagcgcc gtgcgccaac gcttgccatt gtcgaaatat ctatacatta tgttcttgtg
    13921 catcgacaag gcgccgtgcg ggcgccggct accagggagg caggcatgtc cacagagctc
    13981 accgacgcca tcgtcggcat gcgcgaagac gaggcgatgc gcctggtcgc agccatgctg
    14041 gacgccggcg ccgaccccgc tgatgtgctc gaggaggcca aggcggccat gacggtcctc
    14101 ggcgacaagt tcgagcgcga agaggtcttc atccccgagc tcatcatggg cggcgagatc
    14161 atgaagggca ttgccgacga gttgaagccg cggatcaagg gcgaggcggc ggcggagcac
    14221 cggggcacgg tcgtgctcgg caccgtcgcc ggcgacatcc acgacatcgg caaggacgtg
    14281 gtcgtgctca tgctcgacgt caacggctac gacgtccacg acctcggcat cgacgtgccg
    14341 atcgagaagt tcgtcgcggc cgtcagggag ctcaagccgc aggtggtcgg cctgtccggc
    14401 ctgctcacgc tcgctttcga cgcgatgaag gccacggtcg ccggcatcgc cgacgcgggg
    14461 ctccgtgacg aggtcaggat catgatcggg ggctcgccgg tcgacgagca gatctgcgcc
    14521 tacaccggcg ccgacggctg gggccgcgac gcggccgccg cgctgcgcat ggccgccgag
    14581 tggaccgggg gtgcggcatg accccgacgc ccgaggagct ctacgcccag cgcgagcagc
    14641 gcttcaacga cgcggtcgcg ctcaagaaga ccgaccgcgt gcccgtcatg ccgctctacg
    14701 tgcacaactt cccgacgctg atcaaaggca tctccaacaa ggacgccggc tacgaccacg
    14761 agacccgctg gcagtccacc aaagaggcca cgctccgctt cggctggaac ttcgcgccca
    14821 ccaacgacgt gctcgcctcc gacttcttcg ccgcctacgg caacaagcag atcatgtggg
    14881 ccggggatga gggcggctta gccgacgacg caccgttcca gtgggtcgag gacgagtacg
    14941 tcaaggccga cgagctggcc gagttcatcg ccgatcccaa cgggttcacc ttcaacaaga
    15001 tcctgccgcg catggggtct aagctcgagg gcctcggcca gatccccctg ccgccgctgt
    15061 actggttctc caacagctac tacctgcagg tgctcggcaa catgctcggc accccgccgc
    15121 tcaaagccgc cctgcaggcg atgctcgaca tggctgaggc gcaggagaag accaacgccg
    15181 ccaacggcaa gtatctccag gagatgaagg acctcggcta cccgccgacc tggggcgcgg
    15241 cgctcatgcc ggcgttcgac gtcgtctcgg acacgttccg cagcctgcgt ggcagcacgc
    15301 tcgacatgta ccgtcagccg gacacgctgc tggaggcgat cgccgtgatg caggaggcga
    15361 ccctgggcct gttgcacgcc gccttcgccg tcaccggcat cccccgccag ttcatcccca
    15421 tgcacaaggg cgccggcggg ttcatgagca acgagcagtt cgcgaagttc tactggccga
    15481 ccttccgggc gctcatcgac gagtgcatcg ccgcgggcat cacgccgatg ccgctgttcg
    15541 agggcgacta caccgcgcgc ctcgagttcc tcgccgagtt gccgccgggc aaggtcgcgg
    15601 cgcacttcga tcgcatggac cgcaagaagt tcaaggaggc ctgcggcgac gtgctgtgct
    15661 tctggggcaa cgtgcccagc tcgctgctcg tcaccggcac tccgcagcag gtcaaggacg
    15721 acgtcaagga gctcatcgac ctgttcggtg agaccgggct gatcctcgac ggcccgggcg
    15781 ggatccccga ccaggcgaag ccggagaacg tcgaggcgct ggccgaggcc gtggaggagt
    15841 acgggacgct ttgaccgctg cgaacgccgg ccgcgccctc gcgttggaga gaggtacgat
    15901 cgccctgggt gggactccgg gcgaaccgcg cggagaccct cgcctcaagg cgctgtgcag
    15961 gggcgaggcg caactggagg agccgggctt ggctgggacc caaagggagg aaccgtgaca
    16021 caggagctga cacaggccat cgtcgatctg cgggaggacg atgccctggc gatggtcgac
    16081 gacctgctgg cgagcggcgc cgacgcggtc ggcatcctcg ccgactgcaa agcggccatg
    16141 gacgtgatcg gggagaagtt cgcgggcggc gaggccttca tccccgagct catcatggcc
    16201 ggcgagatca tgacgggggt ctccgcgaag ctgaagccgc atctggtcgg ctcggcgccg
    16261 gaggagaagc tcggcgtcat cgtgatcggg accgtccagg gcgacatcca cgacatcggc
    16321 aaggacatcg tcgcgacgat gctcgacatc gccgggttcg acgt
//
LOCUS       DASUMX010000348         2646 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_2010910, whole genome shotgun sequence.
ACCESSION   DASUMX010000348 DASUMX010000000
VERSION     DASUMX010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2010910"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2181
                     /locus_tag="VFZ86_16185"
     CDS             <1..2181
                     /locus_tag="VFZ86_16185"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX5643851.1"
                     /translation="PAKFRPPTMRVELVERRSLITTLSGTAEPLVLLCAPAGTGKTTT
                     LALWAAADERPVVWLQLEEADDDPVVLLLQLVRALGAVMAIDPAVEASLELPVPPVHE
                     RVLPLLDEALAEATPFLLVLDDAHLLAGAKCWDVVAFVLRGLPPGAQLALGTRAEPPL
                     ALARMRTAGEVAEFRAADLAFDRGEAEHLLRAHACGPIDDATLDSLFEVTEGWAAGLR
                     LACRASCDAEQGQWLPRVRGDRREIATYLTAEVIDCQPEHMQEFLLRVSVLRELTPAL
                     CRSVSGRDDAGVLLEEAARDELFIVPSGAAGNRYRFHHLFAEVLGGELERRRPGEAAD
                     LHRKAAAWYARHEDPDATIHHLLLAGDVAAAGDVVAASWLQVWTRGQVETVRRWLASF
                     SDREILAHRALTLTAGWVYTALDAGELGARWGRAACSAPMTDEPSPDGAASLRSSQAL
                     LRATIALDGVRRMREDAELAAGLETTADSSWYADAQVALGVARWLSGSGRRARHPLTL
                     GVGAGSAHNPAAELAALGYLALIAIGDAEWEAAEEHEARARARLAELGFGTNRRCLPM
                     LLARAELLARDPQADLDDAEADVQRILEHMVPHPWMALLAHVVLGEVTLERGDRRKAE
                     AHAAAADAVLRRYPDAGILRRRAGRLRSALDAARLAEPLTGAERRVLELLPTHLTEAR
                     MAEQLFVTRNTVKTHVKSVYRKLGVASRAEAVRRAREIGLLPPD"
     gene            2461..>2646
                     /locus_tag="VFZ86_16190"
     CDS             2461..>2646
                     /locus_tag="VFZ86_16190"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5643852.1"
                     /translation="MDFRYTTEQEMYRQAIREWLAKNLEPRARAIDEAEDGIPDDITA
                     GMAELGMFGTTIPEEYGG"
BASE COUNT          338 a          959 c          971 g          378 t
ORIGIN      
        1 ccggcgaagt tccgtccgcc cacgatgcgc gtggagctcg tcgagcgccg ctccctcatc
       61 acgacgctct ctggtaccgc cgagcccttg gtcctcctct gcgcccccgc ggggaccggc
      121 aagacgacaa cgctcgcgtt gtgggcggcg gccgacgagc gtcccgtcgt ctggctgcag
      181 ctggaggagg ccgacgacga cccggtcgtg cttctcctcc agctggtacg ggctctcggc
      241 gccgtcatgg ccatcgatcc ggccgtggag gcatcgctgg agctgccggt ccctccggtg
      301 cacgagcgcg tgctgccgct gctggatgaa gctctcgccg aggcgacgcc cttcctgctt
      361 gtgctggacg acgcccacct gctcgccggc gccaagtgct gggacgtcgt cgccttcgtg
      421 ctccgcgggc tgccgccggg cgcgcagctg gcgttgggta cgcgcgcgga gccgccgctc
      481 gcgctcgccc gcatgaggac ggcgggcgag gtcgcagagt tccgcgccgc cgatctcgcc
      541 ttcgaccgcg gagaggccga acatctcctg cgggcgcacg cgtgcggacc gatcgatgac
      601 gcgacgctcg actccctgtt cgaggtgacc gaggggtggg cggccggtct gcgcctggcg
      661 tgccgcgcgt cgtgcgacgc ggagcaaggg cagtggctgc cgcgggtgcg gggagacagg
      721 cgcgagatcg ccacctacct caccgcggag gtcatcgact gtcagcctga gcacatgcag
      781 gagttcctgt tgcgcgtctc ggtgctgcgt gagctcactc ccgcgctgtg ccggtccgtc
      841 tcgggacgag acgacgcggg ggtgctcctc gaggaggcgg ctcgcgacga gttgttcatc
      901 gtcccttccg gcgcagccgg aaaccgctac cgcttccatc acctcttcgc ggaggtgctg
      961 ggcggggaac tcgagcgtcg ccgtccgggc gaggccgcag acctgcatcg caaggccgcc
     1021 gcctggtacg cccggcacga ggatccggat gccaccatcc accatctgct cctggcgggc
     1081 gacgtcgccg ccgccggcga tgtcgtcgcg gcgtcctggc tgcaggtgtg gacccgcggc
     1141 caggtcgaga ccgtgcggcg ctggctcgcg tcgttcagcg accgagagat cctcgcgcac
     1201 cgagcgctca cgctcacggc cgggtgggtg tacacggcgc tcgacgccgg ggagctcgga
     1261 gcgcggtggg gcagggccgc ctgcagcgcg ccgatgaccg atgagccgtc acccgacggc
     1321 gccgcctcgc tgcgctcgtc gcaggcgctc ctgcgcgcca ccatcgccct cgacggcgtg
     1381 cgtcgcatgc gcgaggacgc cgagcttgcg gcggggctcg agacgaccgc ggactccagc
     1441 tggtacgccg acgcgcaggt ggcgctcggc gtggcgcgct ggctctcggg ctcgggccgg
     1501 cgcgcacggc acccgctcac cctcggcgtc ggcgcgggtt cggcgcacaa cccggccgcg
     1561 gagctggctg ctctcggcta cctcgcgctg atcgccatcg gggatgcgga atgggaggcg
     1621 gccgaggagc acgaggcgcg cgccagggcg cgcctcgcgg agctcggctt cggcaccaac
     1681 cgccggtgcc tgccgatgtt gctggcgcgc gccgaactgc tggcgcgcga tccgcaggcc
     1741 gacctcgacg acgccgaggc cgacgtgcag cggatcctcg agcacatggt gccgcacccc
     1801 tggatggcac tgctcgccca cgtggtgctc ggggaggtga ctctcgagcg cggcgaccgg
     1861 cgaaaggccg aggcgcacgc cgcggccgct gatgcggtgc tccggcggta tcccgacgcg
     1921 gggatcctgc ggcgccgcgc cgggcgcctg cgcagcgcgc tcgacgcggc gcgcctggcc
     1981 gagccgctga ccggcgcgga gcgccgggtg ctggagctgc tcccgactca cctgaccgag
     2041 gcacggatgg ccgagcagct cttcgtgacg cgcaacacgg tcaagacgca cgtcaagagc
     2101 gtctaccgca agctgggggt cgcgtcgcgc gccgaagccg tgcggcgcgc gcgagagatc
     2161 ggtctgctgc ctccggactg agggtgaggg ctgctgatgc gtcacagtca ggggcgcggc
     2221 cggcgcacgg ccccgtagtc gtacagccgg ccggatcttc ctccaccttg tcgagacggc
     2281 gggcttgcgc ggccacgccc gcccggccta tgatgccgat gctgcacaca tgaccgcaga
     2341 cgggcgccgt ctccgcccgc tgctccacag gtcatggacc cccagcgtgc gcttcggcgt
     2401 ccctgggggt cttcgtcgtt tccgggggcg ttctacaccg gtccctgagg agggaactgc
     2461 atggacttcc gttacaccac cgagcaggag atgtaccgcc aggccatccg cgagtggctg
     2521 gccaagaacc tcgagccgcg cgcccgcgcc atcgacgagg ccgaggacgg catcccggac
     2581 gacatcaccg ccggcatggc cgagctgggc atgttcggca ccaccatccc cgaggagtac
     2641 ggcggc
//
LOCUS       DASUMX010000349         3672 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1290566, whole genome shotgun sequence.
ACCESSION   DASUMX010000349 DASUMX010000000
VERSION     DASUMX010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3672
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1290566"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(292..1044)
                     /locus_tag="VFZ86_16195"
     CDS             complement(292..1044)
                     /locus_tag="VFZ86_16195"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HEX5643853.1"
                     /translation="MDPSAPHAAPTVLLLGGTGRTGGRVLQQLLGRGVHVRALVRSAE
                     RLPAGVAGDPLLTVVETDLLSLSDELVKEQVAGCDVVISCLGHTISARGVFGKPRDLV
                     TRSVERVCRAARDLRPAQPVRLILMSTVSVNRPGKADTRRGWLERAALWLLRGLVPPA
                     RDNQRAADFLHDVVDAADRTAEWVVVRPDTLRDGDVTNYVLHEGLVTSLSHPGETNRA
                     NVAYFMCELATDAEAWAAWRGKLPVIVNATPE"
     gene            complement(1044..1265)
                     /locus_tag="VFZ86_16200"
     CDS             complement(1044..1265)
                     /locus_tag="VFZ86_16200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020630544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX5643854.1"
                     /translation="MGKPTRVTNRIRALRFAAGEMTQAELADRVGVTRQTVIAIEQGK
                     YSPSLEMAFQIARVFGVPLDDVFQYPKEK"
     gene            complement(1268..1717)
                     /locus_tag="VFZ86_16205"
     CDS             complement(1268..1717)
                     /locus_tag="VFZ86_16205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643855.1"
                     /translation="MPYEEKVTWVSLVVTGVVPVAYFAIMLGRLQDVSAADIAYQWPL
                     LIAIGTSIVLTVVGSILAGIGTGISAELHGRDAAGEFGNDERDKEISRRGDRIGFSVS
                     SVGAVGVLALTMLEYEYFWIASALYLSFVVGTLVASVVKIVSYHRGF"
     gene            complement(1809..2528)
                     /locus_tag="VFZ86_16210"
     CDS             complement(1809..2528)
                     /locus_tag="VFZ86_16210"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX5643856.1"
                     /translation="MTPALPLPTPRVVVFDLDYTLLRPTDQFEAPGYVRTGAGFGLAL
                     DPARWPQAERAAYKAVAARREETGLVHDDQLLPLIAHAIIRGLGGGPHGAVELAARAI
                     ISAWSDAENFGLYEDVLPCLDALRAAGVRMALLSNALGHGVEEIVAHFALDEYMDAAV
                     SSLETGAVKPAPRMFSTLFGLLGVSARDAVMVGDSVEDDVEGAVACGCPAILLDRSGR
                     RRDLPVPRIESLAELPAALGL"
     gene            complement(2525..3535)
                     /gene="lipA"
                     /locus_tag="VFZ86_16215"
     CDS             complement(2525..3535)
                     /gene="lipA"
                     /locus_tag="VFZ86_16215"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015109443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEX5643857.1"
                     /translation="MAAGRDNPGAGGGPAAEPGGPPPSPGRKPAWLKHKAPEPRQYRA
                     TGALLDELDLHTVCREARCPNKGECYSSGTATFLILGDVCTRACAFCAVEGEGKPAPR
                     RRAAAAAPSPSGVCALDEDEPRRVAEAARRLGLRHVVITSVTRDDLPDGGAAQFAAAV
                     AAVRAAVPAATVEVLVPDLGGDEAALRAVLAVRPDVLNHNLETVPRLYPRVRPQARYD
                     RSLVLLARAAAWARSGSAAEPAGAPARPLVKTGLMVGLGESDDEVAAVLADAAAAGVD
                     AVTVGQYLQPRAGCLPVARYASPEEFAAYRRRGEAFGLTVVAAPFVRSSYKAGELLEG
                     GA"
     gene            complement(3538..>3672)
                     /locus_tag="VFZ86_16220"
     CDS             complement(3538..>3672)
                     /locus_tag="VFZ86_16220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006187174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643858.1"
                     /translation="ELALAAHSGLTLADVAATIHAHPTLSEAVGEAALGGLGRGVHSI
                     "
BASE COUNT          522 a         1332 c         1342 g          476 t
ORIGIN      
        1 agggcgccgg cgcgcgccag gcgttccgtg aggccggagc ccgctgcggg gcctgctccg
       61 gcgcctcgcc tgttccgctt gttctcgctc tgagtcactg tcatcttctc ctgctgccgg
      121 gtggtctcgc gccggcggcc gtgacgggcc aaagcggcgc atggcaagcc cgggctcgtc
      181 gttcaagggg tcggccgtgc accacacacg acctcgatgc gagcacggct gcgtcaggcg
      241 tcggccatgc cggtggagct gcgcgcggcg gcggtcaccg ccgcggtggg gtcactcagg
      301 cgttgcgttc acgatcacgg ggagcttgcc gcgccacgcc gcccacgcct cggcgtccgt
      361 cgccagctcg cacatgaagt aggcgacgtt ggctctgttg gtctcgccgg ggtgagacag
      421 gctggtcacg aggccttcgt gcagaacgta gttcgtgacg tcaccgtccc tcagtgtgtc
      481 gggccggacg accacccact ccgccgtccg atcggccgca tcgacgacgt cgtggaggaa
      541 gtcggcggcg cgctggttgt cccgcgccgg cgggacgagg ccgcgcagca accagagggc
      601 cgcacgctcg agccaccccc gccgcgtgtc ggcctttccg ggccggttca cagacacggt
      661 gctcatgagg atgagcctca cgggctgcgc gggccgcagg tcccgggccg cgcggcagac
      721 ccgctccacg gagcgggtga ccaggtcgcg cggcttgccg aacacgcccc gcgcgctgat
      781 cgtgtggccc agacaggaga tcacgacgtc gcagccggcg acctgctcct tgaccagctc
      841 gtcgctcagg gagagcaggt cggtctcgac gaccgtgagc agcgggtcgc ccgcgacgcc
      901 ggccgggagc cgctcggccg accggaccag ggcgcgcacg tggacgccgc ggcccagaag
      961 ctgctgcagg acgcggccgc cggtccgccc ggtgccgccg agcagcagca ctgtcggcgc
     1021 ggcatgaggt gcagaggggt ccatcacttc tccttcggat actggaacac gtcatcgaga
     1081 gggacgccga acaccctcgc gatctggaag gccatctcga gcgacggcga gtacttgccc
     1141 tgctcgatgg cgatcaccgt ctggcgcgtc acgcccacgc ggtcggcgag ctcggcttgc
     1201 gtcatctcgc cggccgcgaa ccggagcgcg cggatgcggt tggtgacccg tgtgggcttg
     1261 cccatggtca gaagccccgg tggtacgaga cgatcttgac cacggacgcg accaaggtcc
     1321 cgacgacgaa cgacaggtac agcgcgctgg cgatccagaa gtactcgtac tccagcatgg
     1381 tcagcgcgag cacgccgacc gcgccgacgg aggagacgga gaagccgatc cggtcgccgc
     1441 gacggctgat ctccttgtcc cgctcgtcgt taccgaactc gccggcggcg tctctgccgt
     1501 gcagctcggc ggagatgccg gtgccgatgc ccgcgaggat cgacccgacg acggtcagga
     1561 cgatcgacgt cccgatggcg atgagcagcg gccactggta ggcgatgtcg gcggcggaca
     1621 cgtcctgcag ccgtccgagc atgatcgcga agtacgccac ggggacgacc cccgtgacga
     1681 ccaggctgac ccacgtgact ttctcttcgt acggcacggc tgcctcctcg ctgtgactga
     1741 cgcgatgtca gcaatgtttg acatcgacta ctgtaaagcg caggagacat agtgtcaagt
     1801 attttttact agaggccgag cgccgcaggc aactcggcga gcgactcgat gcgcgggacc
     1861 gggaggtccc ggcgcctgcc ggagcggtcc agcaggatgg cggggcagcc gcaggcgacg
     1921 gcgccctcca cgtcgtcctc cacgctgtcg ccgaccatga cggcgtcgcg cgcggagacg
     1981 ccgagcagcc cgaacagcgt ggagaacatc cgcggcgcgg gcttcaccgc gccggtctcg
     2041 aggctggata ccgcggcgtc catgtactcg tcgagcgcga agtgggcgac gatctcctcc
     2101 accccgtgcc cgagcgcgtt gctgagcagc gccatacgca cgccggccgc gcgcagcgcg
     2161 tccagacagg gcagcacgtc ctcgtacagc ccgaagttct ccgcgtcgct ccaggccgag
     2221 atgatggccc gcgcagcgag ctcgacggcg ccatgcggac caccgccgag cccccggatg
     2281 atcgcgtgcg cgatcagcgg cagcagctgg tcgtcgtgga cgaggccggt ctcctcacgg
     2341 cgcgccgcga ccgccttgta ggcggcgcgc tccgcctgcg gccagcgagc cgggtccagc
     2401 gcgagcccga agccggcgcc ggtgcgcacg tagcccggcg cctcgaactg gtcggtcggg
     2461 cgcagcagcg tgtagtcgag gtcgaagacc acgacccgcg gagtcggcag cggcagcgca
     2521 ggagtcacgc gccgccctcc agcagctcgc cggccttgta ggacgaacgc acgaagggcg
     2581 ccgcgacgac ggtcagtccg aaggcctcgc cgcggcgccg gtacgcagcg aactcctccg
     2641 gcgaggcgta gcgcgccacc ggcaggcagc cggcgcgcgg ctgcaggtac tggccgacgg
     2701 tcacggcgtc cacgccggcc gccgccgcgt cggccagcac ggcggccacc tcgtcgtcgc
     2761 tctcgccgag gccgaccatg aggcccgtct tgacgagcgg gcgggcggga gcgccggccg
     2821 gctctgccgc gctccccgag cgcgcccacg cggcggcgcg cgcgagcagc acgagcgagc
     2881 ggtcgtaccg cgcctgcggc cgcacccgcg ggtacagacg cgggaccgtc tcgagattgt
     2941 ggttgagcac atcggggcga acggcgagca cggcgcgcag cgccgcctca tcgccgccca
     3001 ggtccgggac gaggacctcg acggtagcgg ccggcacggc cgcgcggacg gcggcgacgg
     3061 ccgcagcgaa ctgcgccgcc ccgccgtcgg gcaggtcgtc gcgcgtgacg ctcgtgatca
     3121 cgacgtgccg caacccaagc cgccgcgccg cctccgcgac ccggcgcggc tcgtcctcgt
     3181 cgagcgcgca cacgccgctc gggctcgggg ctgcagccgc cgcgcggcgg cgcggagccg
     3241 gcttgccctc gccctcgacg gcgcagaacg cacacgcccg cgtgcagacg tcaccgagga
     3301 tgaggaacgt cgcggtgccg gacgagtagc actctccctt gttggggcag cgcgcctccc
     3361 ggcacacggt gtgcaggtcg agctcgtcga gcagggcgcc ggtggcgcgg tactgccgcg
     3421 gctccggcgc cttgtgcttg agccacgccg gcttgcggcc agggctcggc ggtgggccgc
     3481 cgggctctgc cgcagggccg ccgcccgcac ccgggttgtc gcggccggcc gccaccctca
     3541 gatgctgtgc acgccgcgcc cgaggccgcc gagcgcggcc tcgccgaccg cctcggacag
     3601 ggtggggtgg gcgtggatgg tggcggcgac gtcggcgagc gtgaggccgc tgtgggccgc
     3661 cagcgcgagc tc
//
LOCUS       DASUMX010000350         5128 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1004957, whole genome shotgun sequence.
ACCESSION   DASUMX010000350 DASUMX010000000
VERSION     DASUMX010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 5128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5128
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1004957"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            103..675
                     /locus_tag="VFZ86_16225"
     CDS             103..675
                     /locus_tag="VFZ86_16225"
                     /inference="COORDINATES: protein motif:HMM:NF020387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HEX5643859.1"
                     /translation="MTTPSARPSRRPTPDGAALLTIRRGGRYLRVADPDWDDPLSGEY
                     ARSRGGRWNPPGAFPVVYLNRDEQVARANVLHRFAGLPYGPEDLDPETAPVLVGADVP
                     RAHYADLVTDEGCAAAGLPTSYPRDESGETVPHQRCQPVGQAVWDAGLPGIACRSAAP
                     AAPAGGEELAWFPRGRRLAVTGVKAFADWF"
     gene            complement(708..2567)
                     /locus_tag="VFZ86_16230"
     CDS             complement(708..2567)
                     /locus_tag="VFZ86_16230"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HEX5643860.1"
                     /translation="MTTLTIAVMARPLARGAVKTRLATALGDGGALAVYERLLRGTLD
                     AAEQVPAVSLVLAEAPAESRSATDPGSPAAVDILAGRSRWKRLGQRGEGLGERLAGVF
                     GALFAGGADAVVIVGSDSPALPPEYLERAFAELAHHDLVLGPAADGGYYLIGAGRQTW
                     SAGAGALARLLAASPMSSPRLLDHTTREARAIGWRMAQLPLWVDVDGPADLGVLERLE
                     GRSALRGEPLGGLREVYLHVTHRCGRACRHCYDGAASADEELTTAEWRDAIDQCVALG
                     ARSFVLIGGDPLARKDFVQLVDHITGAREAKARFFFNSLVDERLAAELSRAGRGLLTP
                     LVSIDGPRAINDVLRGPGSHDDVMASVRNLLTVGLEPVANTVLVRPALPGLTQLAHEL
                     RAAGIGRLHLILPHQRGMTWGSPADGTRGGCVPAEGLIPDGAELVAAVGELLSVAAEI
                     GLTVDNLTGWRRRVGRRNDLCAAGCRDLSIDPAGRVHACVITAGDPAFVAGSLREQPL
                     EEIWRTSSSLRLLRAASARDRAECLACPVADACGGECWVQAHYAARARGEAAGYAAPF
                     PYCDLVRPLLEELSAEAARAGAAVPAGDACAAAGACGGQSSAGADDFALFDCI"
     gene            complement(2609..3904)
                     /locus_tag="VFZ86_16235"
     CDS             complement(2609..3904)
                     /locus_tag="VFZ86_16235"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HEX5643861.1"
                     /translation="MKLLKPPRSRAYLGLAVLAAGLVGLALTGDLLVHATWFTAFYVL
                     AGAGFALLATAATSLPLRGAIVAAVVLRLIFLPGMPSLSDDYHRYVWDGRVQLAGINP
                     YRYRPYEQALDRVRYAGRDRINHDELRTIYPPLTQAVFLGVAAASQALDLETPATPRA
                     EVVLLKLVFGAFDLATAAAIWFLAARRLRLQATVLYLLCPAVIVQTWASAHAETAAML
                     LCVLAAALLVRRRDGWAGIALGLAVAFKVTPLGLLAPALLDGRASPVRFLAGFAPAFL
                     LPYVPYLATGGAFGSLFQSGTGWTGGSLVFTLLARLTTPELARWLALALFIGGAVWIA
                     GSFRGRERTAEAFAWTSTLLILCLPVVHAWYWLTPLALGLAAGLWLPLLIAMAAPLVE
                     SLPMAADLARLRAGLGAVVVRRPGLGRHARRASSKAFRR"
     gene            3883..4617
                     /locus_tag="VFZ86_16240"
     CDS             3883..4617
                     /locus_tag="VFZ86_16240"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5643862.1"
                     /translation="MAASAASSAPDYYCIVVPVLDEAEALPALLAELRGLGLLGRTLF
                     VDNGSCDGGPDLILAAGGRLLREPRRGYGYPCLTGARAAAAAGARAVVFLEADGTDDP
                     SQVKHLVGPVLSGVADLVIGSRRRAVRGAAAGHMPLHQRLGNDWLALNLRLLFGLRLS
                     DDGPFRAVRTDLLERLRLEERAYAFPTEMAVKARLSGARIAVRDTLYRQRAGSSKIAG
                     TWRGSALAVRDISWCLLRLRLFGFRA"
     gene            complement(4655..>5128)
                     /locus_tag="VFZ86_16245"
     CDS             complement(4655..>5128)
                     /locus_tag="VFZ86_16245"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HEX5643863.1"
                     /translation="TRETRSELMLAALRVLMGVIFLAVWADNLIKGYYSPSGWADFVQ
                     EYADTTKVGLYADLLNAVMIPNAAVFAYGQFAIEFVVFGAFLVLGLFTPVSALLGAGF
                     QLNLLLATSGTGEWPGTYIIMCLLLVAVGLAQSGRTLGLDALLARKAPHPRLPVY"
BASE COUNT          725 a         1904 c         1908 g          591 t
ORIGIN      
        1 ggctgctcgc ctcggtccgc gagtcgttcg actacgcgac gacggcgtga ccgcgactgg
       61 acgaccggga cgcagcgtgc gccaggtcgt gaaggcgccc tcgtgacgac gccctctgca
      121 cgaccgtctc gacgccccac cccggacggc gccgcccttc tcaccatccg ccggggcggg
      181 cgctacctgc gcgtcgccga cccggactgg gacgacccgc tgagcggcga gtacgcccgg
      241 tcgcgcggcg ggcgctggaa cccccccggc gcgttcccgg tcgtgtatct caaccgagat
      301 gaacaagtcg cccgcgcgaa cgtgctgcac aggttcgcgg gcctgccgta cggccccgaa
      361 gacctcgacc cggagacggc tcccgtcctc gtcggcgccg acgtcccccg cgcccactat
      421 gctgacctcg tcaccgacga gggctgcgcg gccgccggtc tgccgacgag ctacccgcgc
      481 gacgaatcag gcgagactgt cccgcaccag agatgccagc cggtgggaca ggccgtctgg
      541 gacgcgggct tgcccggcat cgcctgccgc agcgccgcgc cggccgcccc ggccggcggc
      601 gaggagctgg cgtggttccc ccgcggccgc cggctcgcgg tgaccggcgt caaggcgttc
      661 gccgactggt tctaggtgcg cgggtcacgc ccgcgccgtc agtatcctca gatgcagtcg
      721 aagagggcga agtcgtcggc gccggccgag gattggccgc cgcaggcgcc ggcggcggcg
      781 caggcgtcgc cggccgggac ggcggctccg gctctggccg cctcggccga gagctcctcg
      841 agcagcgggc gcacgaggtc gcagtacggg aacggcgcgg cgtagcccgc ggcctcgccg
      901 cgggcccggg ccgcgtagtg cgcctgcacc cagcactcac cgccgcaggc gtcggcgacc
      961 gggcaggcga ggcactcggc ccggtcacgg gcgctcgcgg cgcgcagtag ccgcaggctg
     1021 ctcgacgtgc gccagatctc ctcgagcggc tgctcgcgca gcgagccggc gacgaacgcc
     1081 gggtcgccgg ccgtgatgac gcaggcgtgc acgcggccgg ccggatcgat cgagaggtcg
     1141 cggcagcccg cggcgcagag atcgttgcgc ctgccgacgc gccggcgcca gcccgtcagg
     1201 ttgtccacgg tcagcccgat ctccgcggcg accgacagca gctcgccgac agccgcgacc
     1261 agctcggctc cgtctgggat gagcccctcc gccggcacgc atccgcctcg ggtgccgtcg
     1321 gccggcgatc cccatgtcat gccgcgctgg tgcggcagga tgaggtgcag ccgtccgatc
     1381 ccggcggcgc gcagctcgtg cgccagctgt gtgaggccgg gcagcgccgg tcgcaccagc
     1441 acggtgttgg ccaccggctc caggccgacc gtgagcaggt tgcggacgga ggccatgacg
     1501 tcgtcgtggc ttcccgggcc gcgcaggacg tcgttgatcg cgcgcggccc gtcgatgctc
     1561 accagaggcg tgaggaggcc gcgcccggcg cgactcagct cggccgccag gcgttcgtcg
     1621 accaggctgt tgaagaagaa ccgcgccttc gcctcgcgcg cgccggtgat gtggtccacg
     1681 agctgcacga agtccttgcg cgccagcggg tcgccgccga tgagcacgaa gcttcgcgcg
     1741 cccagggcga cgcactgatc gatcgcgtcg cgccactcgg ccgtcgtcag ttcctcgtcg
     1801 gccgaggccg cgccgtcgta gcagtgccgg caggcgcggc cacagcggtg cgtcacgtgc
     1861 aggtacacct cgcgcaggcc gccgaggggt tcgccgcgca gagctgaccg gccctcgagc
     1921 cgctccagaa cgccgaggtc cgccggtccg tcgacgtcga cccacagcgg aagctgcgcc
     1981 atgcgccagc cgatggcacg cgcctccctc gtcgtgtggt ccaggagcct cgggctgctc
     2041 atcggagacg ccgcgagcag gcgagcgagc gccccggcgc cggcagacca ggtctgccgg
     2101 ccggcgccga tcaggtagta cccgccgtcc gcggccggcc ccagcacgag gtcgtggtgc
     2161 gccagctcgg cgaacgcccg ctcgaggtac tccggtggga gcgccgggct gtcgctgccg
     2221 acgatcacga cagcgtccgc gccgcctgcg aagagcgcgc cgaagacgcc ggccagccgc
     2281 tcgccgagcc cctcgccgcg ctgcccgagc cgcttccagc gcgagcggcc cgcgaggatg
     2341 tccaccgcgg cgggagaccc agggtccgtg gcggaccgcg actccgccgg cgcctcggcc
     2401 agcaccagcg agaccgccgg gacctgctcg gcggcgtcca gcgtgccgcg cagcaggcgc
     2461 tcgtacactg cgagggcgcc gccatccccg agcgccgtcg ccaggcgcgt cttgacggcg
     2521 ccgcgcgcca gcgggcgcgc catcactgcg atggtcaggg tggtcatcgc ctcagtatgg
     2581 cgacggctcc gcccggcgac aacagggctc accggcggaa ggctttggag gatgcccggc
     2641 gggcatgacg cccgagcccg ggacgccgga cgaccacggc cccaagtccg gcccggagac
     2701 gcgccaggtc ggccgccatc ggcagcgact cgacgagcgg cgccgccatc gcgatgagga
     2761 gcggcagcca cagacccgcg gcgagcccca gcgcgagcgg cgtgagccag taccacgcgt
     2821 ggaccaccgg cagacacagg atgagcaagg tggaggtcca ggcgaaggcc tccgcggtcc
     2881 gctcgcgacc gcggaagctc ccggcgatcc acaccgcccc gccgatgaag agcgcgagcg
     2941 ccagccagcg ggccagctcg ggcgtggtga gccgggccag cagcgtgaag acgagggaac
     3001 cacccgtcca gccggtaccg ctctggaaca acgagccgaa ggccccgccg gtggcgaggt
     3061 acggcacgta ggggagcagg aacgccgggg cgaagccggc gaggaagcgg acgggcgacg
     3121 cccgcccgtc cagcaacgcc ggcgccagca ggccgagcgg cgtgaccttg aacgcgacgg
     3181 cgaggccgag cgcgataccg gcccagccgt cgcgccggcg cacgagcagg gcggcggcga
     3241 gcacgcagag cagcatcgcc gccgtctccg cgtgcgcaga cgcccacgtc tgcacgatca
     3301 ccgcggggca gaggaggtac agcaccgtgg cctgcagacg cagcctcctg gcggcgagga
     3361 accagatcgc cgcggcggtc gcgaggtcga acgcgccgaa cacgagcttg aggagcacga
     3421 cctcggcgcg cggcgtcgcc ggcgtctcga gatccagagc ctgggacgcc gccgccacgc
     3481 cgaggaagac cgcctgcgtg aggggcgggt agatggtcct gagctcgtcg tggttgatgc
     3541 ggtcgcggcc ggcgtagcgg acgcggtcga gcgcctgctc gtacggccgg tagcggtagg
     3601 gattgatgcc ggccagctgc acgcgcccgt cccagacgta gcggtggtag tcgtcgctca
     3661 ggctcggcat gcccggcagg aagatgaggc gcaggacgac ggcggcgacg atggcgccgc
     3721 gcagcggcag gctcgtcgcc gcggtcgcca gcagcgcgaa cccggcgccg gcgagtacgt
     3781 agaatgcggt gaaccaggtc gcgtggacga gcaggtcgcc ggtgagggcg agcccgacga
     3841 gtccggcggc gagaacggcg agcccgaggt atgcgcgaga ccgtggcggc ttcagcagct
     3901 tcatccgcgc cagactacta ctgcatcgtg gtccccgtgc tcgacgaggc cgaggccctg
     3961 cccgcgctcc tggcggagct ccgcggcctc ggcctgctgg gacgcaccct gttcgtggac
     4021 aacggctcct gcgacggcgg accggacctg atcctcgccg ccggcgggcg gctgctgcgc
     4081 gagccgcgcc ggggctacgg ctacccctgc ctgacgggcg cccgtgcggc cgcggccgcg
     4141 ggcgcccgcg ccgtcgtctt cctcgaggcc gacggcaccg acgacccgtc ccaggtcaag
     4201 catctcgtcg ggccggtcct gagcggcgtc gccgatctcg tcatcgggtc gcggcggcgc
     4261 gccgtgcgcg gcgccgcggc cggccacatg ccgctccacc agcggctggg caacgattgg
     4321 ctggcgctca acctgcggct gctcttcggc ctgcggctca gcgacgacgg cccgttccgc
     4381 gccgtccgca ccgacctgct ggagcggctg cggctggagg agcgggcgta cgccttcccg
     4441 acggagatgg cggtgaaggc gcgcctctcc ggcgcgcgca tcgccgtccg cgacaccctc
     4501 taccggcagc gggcggggtc gtccaagatc gccggcacct ggcgcggctc ggcgctcgcc
     4561 gttcgggaca tctcctggtg cctgctgcgc ctgcggctgt tcgggttccg ggcctgaccg
     4621 gggagccggc ggcgcctgga ccgagcgccg cgcgtcagta gaccggcagc cgcgggtgcg
     4681 gggcctttcg cgccagcagc gcatcgagcc ccagcgtccg ccccgactgc gcgaggccga
     4741 cggcgaccag cagcaggcac atgatgatgt aggtgcccgg ccactcgccg gtgccggacg
     4801 tcgcgagcag caggttcagc tggaagccgg cgccgagcag cgccgacacc ggcgtgaaca
     4861 ggccgagcac gaggaacgcc ccgaagacga cgaactcgat ggcgaactgc ccgtaggcga
     4921 acacggccgc attggggatc atcaccgcgt tcagcaggtc cgcgtagagg ccgaccttcg
     4981 tggtgtcggc gtactcctgg acgaagtccg cccagccgct gggcgagtag taccccttga
     5041 tgaggttgtc cgcccagacg gcgagaaaga tgacgcccat caggacgcgc agcgcggcca
     5101 gcatcaactc gctgcgcgtc tcgcgcgt
//
LOCUS       DASUMX010000351         3081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_862098, whole genome shotgun sequence.
ACCESSION   DASUMX010000351 DASUMX010000000
VERSION     DASUMX010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_862098"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(171..2030)
                     /locus_tag="VFZ86_16250"
     CDS             complement(171..2030)
                     /locus_tag="VFZ86_16250"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5643864.1"
                     /translation="MHQQRRLRPFLVLLLVAAVAVLGLAWAMEAAGSESASPAADAGK
                     VIYKVGWNGEPDNINPLIGYTSPAFEIWYLTYDSLVGYDPATLAPMKGEESTGLATDW
                     SVSDDGLTWTFTIRSGVTWHDGQPLTARDVAFTYNYIIQNEMENWTAYTNLIETATAT
                     DDTTVVFECSTPKPDMIRHYVPILPEHVWSKIDPKKAVRGYSNEPPYVGSGPFQAVEW
                     KKDSHVKLVANPDYFGGAPKIDELYWTYFTNVDTMLQDLKSGNIDGCVDLLAGQLQQL
                     ENEPGIEASAIAMDGFNELGFNCYKDGPSKGHPALKDWKFRQALNWAVDLQKAVDVVW
                     LGYTTPGTTIIPPDYYTDPDWHWDPPADVKYTYDPEKAGAALDAAGYPDTDGDGQREY
                     EGEPIELGLIARSESPESQQTAKLIAGWFQDVGIKVNVEVMDEATLTDNHLLNYEGDT
                     FTPDYDMFLWGWYLDFDPGSMLSYFTKDQIENWSDCAWWDPEYEELYVAQGQELDPVK
                     RKEYIDRMQQILYEQTPYIVTDYGPDFEAYNTEKWEGYIAIPDPNGNRLMPPFGNAGN
                     ANFLSIGPKTAVATEESSGGGNTTLWIVIAVAAVAVIVVVLLLVRRRPRAMEE"
     gene            2244..3017
                     /locus_tag="VFZ86_16255"
     CDS             2244..3017
                     /locus_tag="VFZ86_16255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014258725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase family hydrolase"
                     /protein_id="HEX5643865.1"
                     /translation="MRTSIPKSRYDAVIFDLDGVVTQTAAVHAAAWKRLFDAYLAERA
                     ARAGTGPGGAPDAALRPFDRDADYARYVDGKPRYDGVRDFLASRGIELPMGDPSDPPE
                     QETVCGLGNGKNAFFNDEVKEHGVKTFPSTIELVHQLRDAGVKVGLMSSSKNTAMVLD
                     ATGTTDLFEVRVDGVVAAEVGLPGKPDPAMYLETARRLGVEAARSVVVEDALSGVEAG
                     RRGGFGLVIGVDRLGPAAALAEAGADVVVDDLAEVSVTA"
BASE COUNT          434 a         1034 c         1096 g          517 t
ORIGIN      
        1 cgtccgccgc cgcccgcctg ctcgccgccg tccggctggc cgtctccggc gcgctcgagg
       61 acgccggccc ggcgccgaag gcgcacgccg gcgtcgtcgc tccctgacgc aagaaaaggg
      121 cggcgggcgg gcccgtcacc aggcccgccc gccgccccgt cacgctccgg tcattcctcc
      181 atcgcccgcg gccgccggcg caccagcagc aggacgacga cgatcacggc gacggccgcg
      241 acggcgatca cgatccacag ggtcgtgttg ccgccgccgc tgctctcttc ggtggcgacg
      301 gccgtcttgg gcccgatgct gaggaagttg gcgttgccgg cgttgccgaa cggcggcatg
      361 agccggttgc cgttcgggtc cgggatggcg atgtagccct cccacttctc ggtgttgtac
      421 gcctcgaagt ccgggccgta gtcggtgacg atgtacggcg tctgctcgta gaggatctgc
      481 tgcatccggt cgatgtactc cttgcgcttg accgggtcga gctcctgacc ctgcgcgacg
      541 tagagctcct cgtactccgg gtcccaccag gcgcagtcgc tccagttctc gatctggtcc
      601 ttggtgaagt agctcagcat ggagcccggg tcgaagtcga ggtaccagcc ccagaggaac
      661 atgtcgtagt cgggggtgaa ggtgtcgccc tcgtagttga gcaggtggtt gtcggtgagc
      721 gtggcctcgt ccatgacctc gacgttgacc ttgatgccca cgtcctggaa ccagccggcg
      781 atgagcttgg cggtctgctg gctctccggc gactccgagc gcgcgatcag gccgagctcg
      841 atcggctcac cctcgtactc ccgctggccg tcgccgtccg tgtcggggta ccccgccgcg
      901 tcgagcgccg cgccggcctt ctccggatcg tacgtgtact tgacgtccgc cggcgggtcc
      961 cagtgccagt cggggtcggt gtagtagtcg ggcgggatga tcgtcgttcc cggcgtggtg
     1021 tagccgagcc agaccacgtc gaccgccttc tgcaggtcga ccgcccagtt gagcgcctga
     1081 cggaacttcc agtccttgag cgcggggtgg cccttgctgg gcccgtcctt gtagcagttg
     1141 aagcccagct cgttgaagcc gtccatggcg atggcgctgg cctcgatgcc gggctcgttc
     1201 tcgagctgct gcagctggcc ggcgagcaga tccacgcaac cgtcgatgtt gcccgacttg
     1261 aggtcctgca gcatcgtgtc gacgttggtg aagtacgtcc agtagagctc gtcgatcttg
     1321 ggcgcgccgc cgaagtagtc ggggttggcg acgagcttga cgtgcgagtc cttcttccac
     1381 tccacggcct ggaaggggcc ggagcccacg tagggcggct cgttcgagta gccgcgcacc
     1441 gccttcttgg ggtcgatctt ggaccagacg tgctcgggca ggatgggcac gtagtgccgg
     1501 atcatgtccg gtttgggcgt ggagcactcg aacaccacgg tggtgtcgtc ggtcgccgtc
     1561 gccgtctcga tgaggttggt gtaggcggtc cagttctcca tctcgttctg gatgatgtag
     1621 ttgtaggtga aggcgacgtc cctggcggtg agcggctgcc cgtcgtgcca ggtgacgccg
     1681 ctgcggatgg tgaacgtcca ggtgagcccg tcgtcgctca ccgaccagtc ggtggcgaga
     1741 cccgtcgact cttcgccctt catgggcgcc agcgtggccg ggtcgtagcc gacgagcgag
     1801 tcgtaggtga ggtaccagat ctcgaacgcc ggcgacgtgt agccgatgag cgggttgatg
     1861 ttgtccggtt cgccgttcca gccgaccttg tagatgacct tgccggcgtc ggccgccggc
     1921 gaggcgctct ccgagccggc cgcctccatg gcccacgcga gtccgagcac ggccacggcc
     1981 gcgaccaaca gcaggacaag gaacggacga agacgtcgtt gctggtgcac gatctccccc
     2041 tttgatcgct cgatccaatg cgccgtgccc cgttcccccc gcggcgcggc gacgctcact
     2101 cttcatggtc tcacatcggc gccgcctatc caagggctag tacgagcgtg acgagcgcgg
     2161 gtggccggga acgtctagca tgagacaagg ccggcgcggc cggcggacga cgcggaagca
     2221 cgcgaaaggg acgggtgaga ccgatgcgga cgagcatccc gaagagccgg tacgacgccg
     2281 tgatcttcga cctggacggc gtcgtcaccc agacggctgc ggtccacgcg gcggcctgga
     2341 agcggctctt cgatgcgtac ctcgcggagc gcgccgcccg cgcgggcaca ggcccgggcg
     2401 gcgcgccgga cgcggcgctc aggccgttcg accgcgacgc cgactacgcg cggtacgtcg
     2461 acggcaagcc gcgctacgac ggcgtccgcg acttcctcgc ctcgcgcggg atcgagctgc
     2521 cgatgggcga tccgtctgac ccgccggagc aggagaccgt gtgcggcctc ggcaacggca
     2581 agaacgcctt tttcaatgac gaggtcaagg aacacggcgt caagacgttc ccgtccacga
     2641 tagagctcgt ccaccagctg cgcgacgccg gggtcaaggt cgggctcatg tcgtcgagca
     2701 agaacacggc catggtgctg gacgcgaccg gcacgacgga cctgttcgag gtgcgcgtgg
     2761 acggcgtggt ggcggcggaa gtcgggctgc cgggcaagcc ggacccggcg atgtacctgg
     2821 agacggcgcg ccggctcggc gtcgaggcgg cgcgctccgt ggtcgtcgaa gacgccctct
     2881 ccggcgtcga ggccggacgc cgcggcgggt tcggcctcgt gatcggcgtc gaccgtctcg
     2941 gcccggccgc ggcgctggcc gaggccggcg cggacgtggt cgtcgacgac ctcgccgagg
     3001 tgagcgtcac cgcctgacgc gaacgccggc gcctgaggcg ccgcggccgc gcctcagctc
     3061 gtgccgagcc cgaagaaccg c
//
LOCUS       DASUMX010000352        11471 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilia bacterium isolate SMAG_U1584
            k141_1437493, whole genome shotgun sequence.
ACCESSION   DASUMX010000352 DASUMX010000000
VERSION     DASUMX010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961724
            Sequence Read Archive: SRR25158526
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilia bacterium (soil metagenome)
  ORGANISM  Thermoleophilia bacterium
            Bacteria; Actinomycetota; Thermoleophilia.
REFERENCE   1  (bases 1 to 11471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 10:09:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,260
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,171
            CDSs (with protein)               :: 3,171
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 2 of 41
            Pseudo Genes (incomplete)         :: 24 of 41
            Pseudo Genes (internal stop)      :: 17 of 41
            Pseudo Genes (multiple problems)  :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11471
                     /organism="Thermoleophilia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1437493"
                     /isolate="SMAG_U1584"
                     /isolation_source="soil"
                     /db_xref="taxon:2026888"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.65305556 N 112.7463889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..2365
                     /gene="ppk1"
                     /locus_tag="VFZ86_16260"
     CDS             137..2365
                     /gene="ppk1"
                     /locus_tag="VFZ86_16260"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003921.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEX5643866.1"
                     /translation="MRGPQDRTRADEYHGREVVKQSTRAAQEEFTGELEAKAELADAD
                     LRDPSLYINRELSMLDFFERVLDEARDTHNPLLERVKFVGIVGSILGEFFMVRVAGLR
                     QQVEAGVTDASADGYTPQQLLPLAQERALGIMKAARQCFSDLGPELEAAGIHIVDYGA
                     LDAGQRAALDKYYEDQVFPVLTPLAFDPGRPFPHISNMSHNLGVLVRDGNGDDHFARV
                     KVPDSLPRLVPVAAPAGAEAGADGVTHMWFTWLEQVIAANLATLFPGMDVLESHAFRV
                     TRDAELAIQELEADDLLETIEQFVRRRRFGSVIRVTIDEAMPEPVRDILAENLELGGS
                     DIYAVRSPLGLSSLWGAGSIDKPDLKYAPLVQSVPPSLAGLEGSELFAAIRHRDVLLH
                     HPYDSFDPVVEFLRAAAEDPDVLGIKQTLYRVGRNAPAVEQLMEAAANGKQVAVLVEL
                     KARFDEESNIGWAKALEREGAHVVYGLIGLKTHCKALQVVRREGDRIRRYTHLGTGNY
                     NSVTTKQYTDLGYLTADDDIGADANLVFNHLTGGGQATTYRKLLVAPVNLRQGIAERI
                     DREIAHAEAGREAHLIFKMNSLVDKPMIRKLYRASQAGVKVELNVRGMCCLRPGVPGV
                     SENITEQSVVGRFLEHSRIYWFRNGGDDEVLLGSADLMPRNLNRRVEIIFPVQDKGIV
                     RRLRDEILPTYLGDNMKTRVLSADGSWTHVWPKDGEEPLNAQEWFVARAREQALDESE
                     SA"
     gene            2365..2847
                     /gene="sixA"
                     /locus_tag="VFZ86_16265"
     CDS             2365..2847
                     /gene="sixA"
                     /locus_tag="VFZ86_16265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HEX5643867.1"
                     /translation="MRLYFLRHGKAESRAEWRGDDADRPLTAEGEAAMRREARELRAM
                     DLTLDAIVTSPYARARRTAEIVAGELNMRGRVVEDDRLEPGFDVPRLEQLLAALGPVE
                     NVMVVGHEPDFSETVAELVGGGDVVMKKGGLARVDAAAAAAGGGRLVWLLTPSLLGSR
                     "
     gene            2850..4679
                     /locus_tag="VFZ86_16270"
     CDS             2850..4679
                     /locus_tag="VFZ86_16270"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HEX5643868.1"
                     /translation="MEVEAKFLVPDEETLERLVETASLAGYDVEPGESRRDVDTFLDT
                     PHRGLLAAGYYFRRRETGDGVRLTLKQLVAPSDGVLRREEIEALVAADVPVAEWPAGA
                     LRERVEQLIGDEPLEAMLTLSQVRVARVVRRGRKEIAELSLDRVTAGTPDGDLDWLEA
                     EVEARGRGGDDDVAALAAALRDQWGLVPEGRAKFTRALELAGDEDDGALMSAAERAVH
                     EEHASGEGARARRAQALLALDRGLSQVEAGRVAGLSDRRVRYWLARYRVEGAGIYDDE
                     AVDPGTGATAPKKRPDILPSDTMTEAAAKTLKFHLAKMLEHEQGTRLGEDPEELHDMR
                     VSTRRMRMALRVFADYLDPDVLRPVLKGLRRTGRTLGAVRDLDVFNEKTQVYLDGLPD
                     ERAGDLDGLLEAWKAERDKQREHLVTYLDGKRYRRFVERTQELLDGPAEALAPRNIVT
                     PRPQRVAQVLPGVLYKDMAVVWAFEGQLGGLETPLPRFHALRKACKGLRYTLEFFEDV
                     LGPGARPLIKRVKGLQDHLGDLQDAVVTCGVLRDYITWGTWRHEGHELPGPIEIIVAA
                     GAARYLTARQEEMEHLVLTFPEAWPSVAGNEFSRELAAVIAEI"
     gene            complement(4676..6292)
                     /locus_tag="VFZ86_16275"
     CDS             complement(4676..6292)
                     /locus_tag="VFZ86_16275"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HEX5643869.1"
                     /translation="MTAEAPTRSAPATGAPITAETAAYIDIGTNSARLMVVRFARDHS
                     WTVLSMQKETVRLGEGEFGEVRLLQPEAMERAVTVCARFADLARAHGATRFVTVATAA
                     TREARNAPEFVRLLRETAGLEVHVVSGREEARLIYLGLLSRVHVDGRALVIDIGGGST
                     EVAVGDASGADVVESISLGAIRLAAEGPRPDADGRYKAADYETLKHRVRLTAQHTLRD
                     LGRPDAAAHGTSGTIVNLAAVAARTLHGRTPGRDEPLARKDLRVVAKRLRAMTTAERR
                     ALPGLTPARADIIVAGAAILDALLEDLGIETIVALDDCGLREGLLLDDLGRAGQHEVR
                     HGAGVRERSVLQLARATSFDEPHARQVARLALELFDSARASGLHRLGAEERELLGYAA
                     LLHDIGTFLSYARHHQHTYYLIRHADLVGFDQEELAVMAATAYFHRKALPAPRFEAFS
                     GLDRRSRKVVRRLGAFLRLAEYLDRGHTGTIAHARLRREADGLVLELETAGDWSLEKW
                     AVERRRDSLEKAFGRPLDVVAVPPPGGGRA"
     gene            complement(6294..6521)
                     /locus_tag="VFZ86_16280"
     CDS             complement(6294..6521)
                     /locus_tag="VFZ86_16280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5643870.1"
                     /translation="MTKLKNEFSRKEWKDCCGKGCKKCEIAQAYIAEYGDRKVGLAKL
                     NADRDAVKKAGKKGGKAKGSKKGKAKKKVKA"
     gene            complement(6650..7318)
                     /locus_tag="VFZ86_16285"
     CDS             complement(6650..7318)
                     /locus_tag="VFZ86_16285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005946895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HEX5643871.1"
                     /translation="MASDAKLIIAARAGRELKDGQIINLGIGLPTLVANYVPEGVDVV
                     IQTENGAIGVGPAPQPGCEDRDRANAGNQPISLIPGGSYFDSATSFAMIRGGHIDATF
                     LGTLQVDEKGNIANWIVPGKMLAGMGGAMDLITGAKIVYIVSEHCSKDGSSKLMKQCT
                     FPLTGAEEADVIITERALFRRSAETGWTLEEVAVGYTLDDIAACTDMAYTVADDVRLD
                     AYGA"
     gene            complement(7308..8006)
                     /locus_tag="VFZ86_16290"
     CDS             complement(7308..8006)
                     /locus_tag="VFZ86_16290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010294830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HEX5643872.1"
                     /translation="MPKNKVVPIEEAMSHITSGMSIAVGGFIGQGDPLTLIDYLKTMD
                     VGDLTIYENDAGYRDRGVVELVKQGKVKKIVASHIGTTPEIGKAMNEGRLEVEPTPQG
                     TLAEMMRCGGAGLGGFLTPTGIGTVAEEGKQIVELGGRRYILVPSLKVDVAIVRAHRG
                     DTWGNLIYRGTSRNFNVPAAMCAEYVIAEVENLYTIGELDPEGIHTSGIFVDAVVRAN
                     TEYCVLREEKFNGQ"
     gene            8225..9094
                     /gene="mtnP"
                     /locus_tag="VFZ86_16295"
     CDS             8225..9094
                     /gene="mtnP"
                     /locus_tag="VFZ86_16295"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015053336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HEX5643873.1"
                     /translation="MTAVTESASVGVIGGSGFYELLENAREVVIDTPFGPPSDAYFLG
                     EIDGVRVAFLPRHARGHRILPGEVNYRANIWGMKALGVHYVLSASAVGSLREELAPLD
                     VVVPDQLFDRTKARPSTFFGEGVVVHMGFADPFCPYTSKLIVEAGREAGATVHEGGTY
                     VCIEGPQFSTRAESNVYRQLGFDIIGMTNLQEAKLAREAELCYATMAMVTDYDVWYEG
                     EEDVTLETVLENVRRNVATAQAIVRRAVVALDQQRDCSCRHAVEFAINTPAELIPPAT
                     KQKLDLLIGRYLG"
     gene            9155..11311
                     /locus_tag="VFZ86_16300"
     CDS             9155..11311
                     /locus_tag="VFZ86_16300"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HEX5643874.1"
                     /translation="MSSFVSNNAILVAVVCGLIAVIYGVLLIRWLLKLPAGDEVMQRV
                     AGAVQEGAKAYLGRQYRTVAMVGVVVFVILLVALGLGKDWTYGWHAAVGFVIGAVLSA
                     AAGYIGMMVSVRVNVRTAEAARHGLGRALTVAFRGGAVTGLLVVGLALLGVSIYFWIT
                     GDVAALVGLGFGGSLISVFARVGGGIFTKAADVGADLVGKVEAGIPEDDPRNPAVIAD
                     NVGDNVGDCAGMAADLFETYAVTAVAAMLLAFLTFRGNVNVTGIAKGLLINVDAEVVY
                     KPAVLYPLVIGGVSIIASVIGTFFVRLGKSNNIMNALYKGLAVAAVVAIALFYPITEW
                     MLGGAAATAFGVPSVTRLFLAAVTGIVVTALIVVITEYYTASRYNPVKSIAKASVTGH
                     ATNIIQGLAVGMQSTALPVLVIAAGILVSYEIAGGGAAGLYGIAVAVMAMLSMTGIIV
                     AIDAYGPITDNAGGIAEMANMPELVRQTTDPLDAVGNTTKAITKGYAIGSAAFAALIL
                     FVSYTLDLQEHFLTEYDFEGVLSFGIDNPWVLAGLFIGGMLPYLFASLCMKAVGKAGG
                     QVVEEVRRQFREIKGIMDGSGKPDYATAVDIVTKSALREMLLPAAIPVLTPVIIALVF
                     GSKAYLVLGGVLMGTIITGIFLAISMTSGGGAWDNAKKLIEDGEYGGKGSDAHAAAVT
                     GDTVGDPYKDTAGPAINPMIKVINIVALLVIPFLVK"
BASE COUNT         1649 a         4071 c         4063 g         1688 t
ORIGIN      
        1 agaggtcacc cgccggccgc cccgcgtttc cggcctcatc tcgcgcggac gcgcgcgttc
       61 tgggcatata ctgtcagacc cggacgagaa gagccggagc ggcgccgggc gccctccgga
      121 gtcgaggagg tacgcagtga gggggccaca agaccggaca cgcgccgacg agtaccacgg
      181 cagagaggtc gtcaagcagt cgaccagggc cgcgcaggag gagttcaccg gggagctgga
      241 ggccaaggcc gagctggccg acgccgacct gcgtgatccc tcgctctaca tcaaccgcga
      301 gctcagcatg ctcgacttct tcgagcgcgt gctggacgag gcccgggaca cccacaaccc
      361 gctgctcgag cgggtcaagt tcgtcggcat cgtcgggtcg atcctcggcg agttcttcat
      421 ggtgcgcgtc gccggcctgc ggcagcaggt cgaggccggc gtcacggatg cgtcggccga
      481 cgggtacacg ccgcagcagc tgctgcccct cgcgcaggag cgcgccctgg gcatcatgaa
      541 ggcggcccgc cagtgcttca gcgacctcgg ccccgagctc gaggcggccg gcatccacat
      601 cgtcgactac ggcgcgctcg acgccggcca gcgcgccgcc ctggacaagt actacgagga
      661 ccaggtgttc ccggtgctca cgccgctggc cttcgacccc ggccggccct tcccgcacat
      721 ctccaacatg agccacaatc tcggcgtgct cgtgcgcgac ggcaacggcg acgaccactt
      781 cgcccgcgtc aaggtgccgg acagcctgcc gaggctcgtc ccggtggcgg cgcccgcggg
      841 cgccgaggcg ggcgccgacg gcgtgaccca catgtggttc acctggctgg agcaggtcat
      901 cgccgccaac ctcgccacgc tgttcccggg catggacgtg ctcgagtcgc acgcgttccg
      961 cgtcacccgt gacgccgagc tggcgatcca ggagctggag gcggacgacc tcctcgagac
     1021 gatcgagcag ttcgtgcgcc gccgccgttt cggctcggtc atccgcgtca ccatcgacga
     1081 ggccatgccg gagccggtgc gcgacatcct cgccgagaac ctcgagctgg gcggctccga
     1141 catctacgcg gtccgctcgc cgctgggcct gagcagcctc tggggggccg gctccatcga
     1201 caagccggac ctcaagtacg cgccgctggt gcagtcggtg ccgccgtccc tggcggggct
     1261 ggagggcagc gagctgttcg cggccatccg gcaccgtgac gtgctgctgc accaccccta
     1321 cgactcgttc gatccggtgg tcgagttcct gcgcgccgcc gccgaggacc ccgacgtcct
     1381 cggcatcaag cagacgctgt accgcgtggg ccgcaacgcc cccgccgtcg agcagctcat
     1441 ggaggcggcg gccaacggca agcaggtggc cgtgctggtc gagctcaagg cgcggttcga
     1501 cgaagagagc aacatcggct gggccaaggc gctggagcgc gagggcgcgc acgtggtgta
     1561 cggcctcatc ggcctcaaga cgcactgcaa ggcgctgcag gtcgtgcgcc gcgagggcga
     1621 ccggatccgg cgctacacgc acctcggcac cggcaactac aacagcgtga cgaccaagca
     1681 gtacaccgac ctcggctacc tcaccgccga cgacgacatc ggcgccgacg ccaacctcgt
     1741 cttcaaccac ctcacggggg gcgggcaggc gacgacgtac cgcaagctgc tcgtggcgcc
     1801 cgtcaacctg cgccagggca tcgccgagcg catcgaccgc gagatcgccc acgccgaggc
     1861 gggccgcgag gcgcacctca tcttcaagat gaactccctg gtggacaagc cgatgatccg
     1921 caagctgtac cgggcctcgc aggcaggggt caaggtcgag ctgaacgtgc gcggcatgtg
     1981 ctgtctgcgg ccgggcgtcc ccggggtcag cgagaacatc accgagcaga gcgtggtcgg
     2041 ccgcttcctc gagcacagcc ggatctactg gttccgcaac ggcggcgacg acgaggtgct
     2101 gctgggcagc gccgacctca tgccgcgcaa cctgaaccgc cgcgtcgaga tcatcttccc
     2161 ggtgcaggac aagggcatcg tgcggcgtct gcgggacgag atcctgccga cctacctggg
     2221 cgacaacatg aagacgcgcg tgctgagcgc ggacgggtcc tggacgcacg tgtggcccaa
     2281 ggacggggag gagcccctga acgcgcagga gtggttcgtc gcgcgggcgc gcgagcaggc
     2341 gctcgacgag agcgagagcg cctgatgcgc ctgtacttcc tgcgtcacgg caaggccgag
     2401 agccgggcgg agtggcgcgg cgacgacgcc gaccggccgc tcacggccga gggcgaggcg
     2461 gcgatgcggc gcgaggcgag ggagctgcgc gccatggatc taacgctcga cgcgatcgtg
     2521 acgagcccgt acgcgcgcgc acgccggacc gctgagatcg tcgccggcga gctcaacatg
     2581 cgcggacgcg tcgtcgagga cgaccggctg gagccggggt tcgacgtgcc gcgcctggag
     2641 cagctgctgg cggcgctcgg gccggtggag aacgtgatgg tcgtcgggca cgagcccgac
     2701 ttcagcgaga cggtcgccga gctcgtcgga ggcggcgacg tggtgatgaa aaagggcggg
     2761 ctcgcccgcg tggacgcggc cgcggcggcg gccggcggcg ggcggctcgt gtggctgctc
     2821 acgccgtctc ttctggggag cagatgacga tggaggtcga agccaagttc ctggtaccgg
     2881 acgaggagac gctggagcgc ctcgtcgaga ccgcgtcgct ggcggggtac gacgtcgagc
     2941 cgggcgagtc ccgccgcgac gtcgacacct tcctcgacac cccgcaccgc ggtctgctgg
     3001 cggcgggcta ctacttccgt cgccgcgaga ccggcgacgg cgtgcggctc acgctcaagc
     3061 agctggtcgc gccgtccgac ggcgtgctgc gccgagagga gatcgaggcg ctcgtggccg
     3121 ccgacgtacc cgtggccgag tggccggccg gggccctgcg cgagcgcgtg gagcagctca
     3181 tcggcgacga gccgctggag gcgatgctca cgctctcgca ggtgcgcgtg gcgcgggtgg
     3241 tgcggcgcgg acgcaaggag atcgccgagc tcagcctcga ccgcgtcacc gccggcaccc
     3301 cggacggtga cctcgactgg ctcgaggcgg aggtcgaggc gcgcggccgc ggaggcgacg
     3361 acgacgtcgc cgcgctggcg gccgcgctgc gcgaccagtg gggcctcgtg cccgagggcc
     3421 gcgccaagtt cacgcgcgcg ctcgagctcg ccggcgacga ggacgacggc gcgctcatga
     3481 gcgcggccga gcgcgccgtg cacgaggagc acgcctcggg tgagggcgcg agggcgcgcc
     3541 gcgcgcaggc gctgctcgcg ctcgaccgcg gcctctcgca ggtcgaggcg ggccgcgtgg
     3601 ccggcctctc cgaccggcgc gtccgctact ggctcgcgcg gtaccgggtc gagggcgccg
     3661 gcatctacga cgacgaggcc gtcgacccgg gcacgggcgc gacggccccg aagaagcggc
     3721 ccgacatcct gccctcggac acgatgacgg aggcggcggc caagacgctc aagttccacc
     3781 tcgccaagat gctcgagcac gagcagggca cgcgcctggg tgaggacccc gaggaactgc
     3841 acgacatgcg cgtctccacg cgccgcatgc gcatggcgct gcgcgtgttc gccgactacc
     3901 tggacccgga cgtgctgcgg ccggtgctca agggcctgcg gcgcaccggc cgcaccctcg
     3961 gcgccgtccg cgacctcgac gtcttcaacg agaagacgca ggtctacctc gacggcctgc
     4021 ccgacgagcg cgccggcgac ctggacgggc tgctcgaggc ctggaaggcc gagcgcgaca
     4081 agcagcgcga gcatctcgtc acctacctcg acggcaagcg ctaccggcgc ttcgtcgagc
     4141 ggacgcagga gctgctggac ggccccgccg aggcgctggc gccgcgcaac atcgtcaccc
     4201 cgcggccgca gcgcgtggcc caggtgctgc ccggcgtcct ctacaaggac atggccgtgg
     4261 tctgggcctt cgagggccag ctcggcggcc tcgagacgcc gctgccgcgc ttccacgccc
     4321 tgcgcaaggc ctgcaagggc ctgcgctaca cgctcgagtt cttcgaggac gtgctcggcc
     4381 ccggcgcgcg cccgctgatc aagcgcgtca agggcctgca ggaccacctc ggcgatctgc
     4441 aggacgcggt cgtgacctgc ggcgtgctgc gcgactacat cacctggggc acctggcgcc
     4501 acgaggggca cgagctgccc gggcccatcg agatcatcgt cgctgctggc gccgcccgct
     4561 acctcacggc gcgccaggag gagatggagc acctggtgct gacgttcccc gaggcgtggc
     4621 cgtcggtcgc cggcaacgag ttcagccgcg agctcgcggc ggtgatcgcc gagatctaag
     4681 cgcggccgcc gccgggcggc ggcacggcga ccacgtcgag cgggcggccg aacgccttct
     4741 cgaggctgtc gcgccgccgc tcgacggccc acttctcgag gctccagtcg ccggcggtct
     4801 cgagctccag cacgaggccg tcggcctcgc ggcgcaggcg tgcgtgcgcg atcgtcccgg
     4861 tgtgcccccg gtccaggtac tccgccagcc ggaggaacgc gccgagccgg cggacgacct
     4921 tgcgcgagcg ccggtccagt cccgagaacg cctcgaaccg gggcgcgggg agggccttgc
     4981 ggtggaagta cgcggtggcc gccatcacgg cgagctcctc ctggtcgaac cccacgaggt
     5041 cggcgtgccg gatgaggtag taggtgtgct ggtggtggcg cgcgtagctc aggaaggtgc
     5101 cgatgtcgtg gaggagtgcg gcgtacccga ggagctcgcg ctcctccgcg ccgagccggt
     5161 gcagcccgct cgcgcgggcg gagtcgaaga gctcgagcgc gagccgggcg acctggcggg
     5221 cgtgcggctc gtcgaacgag gtcgcgcgcg cgagctggag cacgctccgc tcgcgcaccc
     5281 cggcgccatg gcgcacctcg tgctggccgg cgcgcccgag gtcgtcgagc agcaggccct
     5341 cgcgcaggcc gcagtcgtcc agcgcgacga tcgtctcgat gcccaggtcc tcgagcagcg
     5401 cgtcgaggat cgcggcgccg gcgacgatga tgtcggcgcg cgcgggcgtc aggcccggca
     5461 gcgcgcgccg ttccgcggtg gtcatcgcgc ggaggcgctt ggccacgacg cgcaggtcct
     5521 tgcgcgcgag gggctcgtcg cgccccggcg tgcgcccgtg cagcgtgcgc gcggcgacgg
     5581 cggcgagatt cacgatggtg ccggacgtgc cgtgggcggc ggcgtccggc cggccgaggt
     5641 cacgcagcgt gtgctgcgcc gtgaggcgca cgcggtgctt gagcgtctcg tagtccgcgg
     5701 ccttgtaacg tccgtcggcg tcgggccgcg gaccctccgc ggccagccgg atggcgccga
     5761 gggagatgga ctcgacgacg tcggcgccgg aggcgtcgcc cacggcgacc tccgtgcttc
     5821 cgccgccgat gtcgatcacc agcgcgcgcc cgtcgacgtg cacgcggctg aggaggccga
     5881 ggtagatcag ccgggcctcc tcgcggcccg agaccacgtg cacctcgagc cccgccgtct
     5941 cgcgcaggag gcgcacgaac tcgggcgcgt tgcgcgcctc ccgcgtggcc gccgtcgcga
     6001 ccgtcacgaa gcgggtcgcg ccgtgggcgc gcgcgaggtc ggcgaagcgc gcgcacacgg
     6061 tgaccgcccg ctccatcgcc tccggctgga gcaggcgcac ctcgccgaac tcgccctcgc
     6121 ccagccggac ggtctccttc tgcatgctga ggaccgtcca ggagtggtcg cgcgcgaagc
     6181 gcacgaccat caggcgcgcc gagttggtgc cgatgtcgat gtacgccgcc gtctcggcgg
     6241 tgatgggcgc gcccgtcgcc ggcgcgctcc gcgtcggcgc ctccgcggtc acgtcaggcc
     6301 ttgaccttct tcttggcctt gcccttcttg gagcccttcg ccttgccgcc cttcttgccc
     6361 gccttcttga cggcatcgcg gtcggcgttc agcttcgcca ggcccacttt gcgatcaccg
     6421 tactcggcga tgtaggcctg ggcgatctcg cacttcttgc agcccttgcc gcagcagtcc
     6481 ttccactcct tgcggctgaa ctcgttcttg agcttggtca ctgtgcgctc ccttcacgct
     6541 ggccggtggt gcatggatac cgcgggggag aggtgcgaaa acgcgggcgg ccggcgggcc
     6601 cgcctccggg accgccggcc cacccggccg tgcatctgtc cgcgcgacgt caggcgccgt
     6661 aggcgtcgag cctgacgtcg tcggccaccg tgtacgccat gtcggtgcac gcggcgatgt
     6721 cgtcgagcgt gtagcccacc gcgacctcct cgagcgtcca gcccgtctcc gcggagcggc
     6781 ggaacagcgc ccgttcggtg atgatcacgt ccgcctcttc ggcgccggtg agcgggaagg
     6841 tgcactgctt catgagcttc gaagagccgt ccttggagca gtgctcgctc acgatgtaga
     6901 cgatcttggc gccggtgatg aggtccatgg cgccgcccat gccggcgagc atcttgccgg
     6961 ggacgatcca gttcgcgatg ttgcccttct cgtccacctg cagggtgccc aggaaggtcg
     7021 cgtcgatgtg gccgccgcgg atcatggcga acgaggtggc cgagtcgaag tagctgccgc
     7081 cggggatcag cgagatcggc tggttgccgg cgttggcgcg gtcgcggtcc tcgcagccgg
     7141 gctgcggcgc ggggccgacg ccgatggcgc cgttctccgt ctggatgacg acgtcgacgc
     7201 cctcggggac gtagttcgcg accagcgtgg gcaggccgat accgaggttg atgatctgcc
     7261 cgtccttgag ctcccggccg gcgcgggcgg cgatgatgag tttcgcgtca ctggccattg
     7321 aacttctcct cgcgcagcac gcagtactcc gtgttggcgc gcaccaccgc gtccacgaag
     7381 atgccgctgg tgtggatacc ttcggggtcg agctcgccga tcgtgtagag gttctcgacc
     7441 tcggcgatga cgtactcggc gcacatggcg gccggcacgt tgaagttgcg gctcgtgcca
     7501 cggtagatga ggttgcccca ggtgtcgccg cggtgggcgc ggacgatggc gacgtcgacc
     7561 ttgagcgacg gcacgaggat gtaccggcgg ccgccgagct cgacgatctg cttgccctcc
     7621 tcggcgacgg tgccgatgcc ggtgggcgtg aggaagccgc cgaggccggc gccgccgcag
     7681 cgcatcatct cggcgagcgt gccctgcggc gtcggctcca cctcgaggcg gccctcgttc
     7741 atcgccttgc cgatctcggg cgtggtgccg atgtggctgg cgacgatctt cttgaccttg
     7801 ccctgcttga ccagctcgac gacgccgcgg tcgcggtagc cggcgtcgtt ctcgtagatg
     7861 gtgaggtcgc cgacgtccat cgtcttcagg tagtcgatca gcgtgagcgg atcgccctgg
     7921 ccgatgaagc cgccgacggc gatgctcatc ccactcgtga tgtgggacat cgcctcctcg
     7981 atggggacca ccttgttctt cggcaccgtt cgctccttag gcagctgcgc gtccgtgaga
     8041 agactatcac cccggcgcgg cgcccccggg cggcggacgg tggacgtcgt ggcacaccgc
     8101 gctgcactgc ccttgccgtt tcgctatgcg gtcaacgcgt ccaccatacg gatgacatcc
     8161 gctgtttgtc cgctcttccc ggcactgtat acttcgcgcg gcgcaacgga aaaggagggc
     8221 ctttgtgacc gcagtcaccg agtctgcaag cgtcggcgtc atcggcgggt ccgggttcta
     8281 cgagctcctc gagaacgccc gcgaggtggt catcgacacc ccgttcggac ctccgtcgga
     8341 cgcctacttc ctcggcgaga tcgacggcgt ccgcgtcgcg ttcctgccgc gtcacgcgcg
     8401 ggggcaccgc atcctgccgg gcgaggtcaa ctaccgcgcc aacatctggg gcatgaaggc
     8461 gctgggggtg cactacgtgc tctccgccag cgccgtcggc agcctgcggg aggagctcgc
     8521 gccgctcgac gtcgtggtgc ccgaccagct cttcgaccgc accaaggcgc gcccgagcac
     8581 gttcttcggc gagggcgtcg tcgtgcacat gggcttcgcc gacccgttct gtccctacac
     8641 cagcaagctc atcgtcgagg cggggcgcga ggcgggcgcc acggtgcacg agggcggcac
     8701 ctacgtctgc atcgagggtc cccagttcag cacccgcgcg gagagcaacg tctaccggca
     8761 gctgggcttc gacatcatcg gcatgaccaa cctgcaggag gccaagctgg cgcgtgaggc
     8821 ggagctctgt tacgccacca tggcgatggt caccgactac gacgtgtggt acgaaggcga
     8881 agaagacgtg acgctggaga ccgtgctgga gaacgtccgg cgcaacgtcg ccacggcgca
     8941 ggcgatcgtc cggcgcgccg tggtcgctct cgaccagcag cgtgactgct cgtgccggca
     9001 cgcggtcgag ttcgcgatca acacgccggc ggagctcatc ccgcccgcga ccaagcagaa
     9061 gctcgacctg ctcatcggca ggtacctggg gtaagggcaa cgacgggcgt gcctcccgcg
     9121 cccgcgtagg aggggttcag aaaggagcga cactgtgtct tcgttcgtat ccaacaacgc
     9181 catactcgtg gcggtcgtct gcgggctgat cgccgtgatc tacggcgtcc tgctcatcag
     9241 gtggctgctc aagctgccgg ccggcgacga ggtcatgcag cgcgtcgccg gcgccgtgca
     9301 ggagggcgcg aaggcctacc tggggcgcca gtaccgcacc gtcgccatgg tgggcgtggt
     9361 cgtcttcgtg atcctgctcg tcgcgctcgg cctgggcaag gactggacct acggctggca
     9421 cgccgccgtc ggcttcgtca tcggcgccgt cctctcggct gcggccggct acatcggcat
     9481 gatggtcagc gtgcgcgtca acgtgcgcac cgccgaggcc gcgcgccacg gcctcggcag
     9541 ggcgctcacc gtggccttcc gcggcggcgc cgtgaccggc ctgctcgtcg tcggcctcgc
     9601 gctgctcggc gtgagcatct acttctggat caccggggac gtcgccgcgc tcgtcgggct
     9661 cggcttcggc ggcagcctca tcagcgtgtt cgcgcgcgtc ggcggcggca tcttcaccaa
     9721 ggccgccgac gtgggcgccg acctcgtcgg caaggtcgag gcgggcatcc ccgaggacga
     9781 cccgcgcaac ccggccgtca tcgccgacaa cgtgggcgac aacgtcggcg actgcgccgg
     9841 catggccgcc gacctgttcg agacgtacgc ggtgaccgcc gtggccgcga tgctcctcgc
     9901 gttcctcacc ttccgcggca acgtcaacgt caccggcatc gccaagggcc tgctcatcaa
     9961 cgtcgacgcc gaagtggtct acaagccggc cgtgctctac ccgctcgtca tcggcggcgt
    10021 gagcatcatc gccagcgtga tcggcacgtt cttcgtgcgg ctcggcaaga gcaacaacat
    10081 catgaacgcc ctctacaagg gcctcgccgt tgccgccgtc gtcgccatcg cgctcttcta
    10141 cccgatcacc gagtggatgc tgggcggcgc ggccgccacg gcgttcggcg tccccagcgt
    10201 cacgcgcctg ttcctggccg ccgtcaccgg catcgtcgtc accgcgctca tcgtcgtgat
    10261 caccgagtac tacacggcgt cgcgctacaa cccggtcaag agcatcgcca aggccagcgt
    10321 gaccggccac gccaccaaca tcatccaggg gctcgccgtc ggcatgcagt ccaccgccct
    10381 gccggtgctc gtcatcgccg ccggcatcct ggtctcgtac gagatcgcgg gtggcggcgc
    10441 ggccggcctc tacggcatcg ccgtcgcggt catggccatg ctctccatga ccggcatcat
    10501 cgtcgccatc gacgcctacg ggcccatcac cgacaacgcc ggcggcatcg ccgagatggc
    10561 caacatgccc gagctggtgc gccagaccac cgacccgctg gacgcggtcg gcaacaccac
    10621 caaggccatc accaagggct acgccatcgg gtcggcggcc ttcgcggcgc tcatcctgtt
    10681 cgtgtcctac acgctcgacc tgcaggagca cttcctcacg gagtacgact tcgagggcgt
    10741 gctctcgttc ggcatcgaca acccctgggt gctcgccggc ctgttcatcg gcggcatgct
    10801 gccgtacctg ttcgcctccc tgtgcatgaa ggccgtgggc aaggccggcg gccaggtcgt
    10861 ggaggaggtc cggcgccagt tccgcgagat caagggcatc atggacggct ccggcaagcc
    10921 ggactacgcc acggccgtcg acatcgtcac caagtcggcg ctgcgcgaga tgctgctgcc
    10981 ggcggcgatc cccgtgctca cgccggtcat catcgcgctc gtgttcggca gcaaggccta
    11041 cctcgtgctc ggcggcgtgc tcatgggcac catcatcacc ggcatcttcc tggcgatcag
    11101 catgacctcg ggcggcggcg cctgggacaa cgccaagaag ctcatcgagg acggcgagta
    11161 cggcggcaag ggctccgacg cccacgcggc cgccgtcacc ggcgacaccg tcggcgatcc
    11221 gtacaaggac accgccggcc ccgccatcaa cccgatgatc aaggtcatca acatcgtggc
    11281 cctgctcgtc atcccgttcc tcgtgaagta agagagggcg gtagagaagg cggcgggcgg
    11341 cctcctgggg ccgcccgccg gcgttccggg ccggagcagc agggtcaacc ggacggcgga
    11401 ccacttgctc aggcgtccgt cggatgccgc tccggtgtta agaacccatc aaggcggttg
    11461 cccgcggttc c
//
